LOCUS       DAEEKQ010000001        81557 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.001, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000001 DAEEKQ010000000
VERSION     DAEEKQ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 81557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 81557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..81557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.001"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..150
                     /locus_tag="KLK57_000001"
     CDS             <1..150
                     /locus_tag="KLK57_000001"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890497.1"
                     /translation="LIDSLLKEVGLSGEGLTALTRPLVMPAPEKVPDVSEPVTAPDNS
                     EPQQV"
     gene            156..473
                     /locus_tag="KLK57_000002"
     CDS             156..473
                     /locus_tag="KLK57_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001330559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilI type IV pilus biogenesis protein"
                     /protein_id="HBE0890498.1"
                     /translation="MPEFFRHLFHKECPHGIFWFKRGLKMSAEKFRVVIYTNTCEKKV
                     SYIPATEDINIAIMPFQTADNGLIIVCQNSRIIRRKERIPGSSCEWKDVTLAPKSRNR
                     VQL"
     gene            1151..2221
                     /locus_tag="KLK57_000003"
     CDS             1151..2221
                     /locus_tag="KLK57_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcpQ domain-containing protein"
                     /protein_id="HBE0890499.1"
                     /translation="MKKKSLTLLLPSLLSGCTAFNATPVPDTTPPALVFVDGQIRESA
                     AIIAHTQKRLLPPPVTRPVTPPRPTTTATPTTAVSSQSTALPPRPLLQATQLPALVMT
                     GKPGQSSVVYLREAHQRSVSDWIRQLLPPGWQFRQQNAAIPALNKRLPVWSVNDQWHR
                     ALNALLAGQQLYGHMDWNRKILTVTTSATPPVDVTAPQGSQKAADTPRNPFRGSAATP
                     AGPTPVTPAVSVSRAQTTTGTSGTPESRTTTPAPVATKPVVPPQQKVIPPRQWKMEKG
                     TTLKDGLARWTSSEKCPHGTWTLRWETPVNYSIDAPLVFTGSFRQALEQLFALYTPAE
                     KPLYARTSTAQCLLRVTDRPQE"
     gene            2225..2662
                     /gene="pilM"
                     /locus_tag="KLK57_000004"
     CDS             2225..2662
                     /gene="pilM"
                     /locus_tag="KLK57_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilM"
                     /protein_id="HBE0890500.1"
                     /translation="MGWVTGFIMVIMAIGNFWFHSHLSRTVHHQQTAEITQQAADFIR
                     YMNAINDYLYQHPERRAAGGQLTSAQLGLPATKNVSHLISQQRVFVWAKEKPGLMGAL
                     LEQSGDSALLARVENGRLLDTRGRRISITLPAVIPDQVIIWMN"
     gene            2694..4313
                     /locus_tag="KLK57_000005"
     CDS             2694..4313
                     /locus_tag="KLK57_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN family type IVB pilus formation outer
                     membrane protein"
                     /protein_id="HBE0890501.1"
                     /translation="MNKKLLAIMIAATLLQGCTSFRQAENTRKDAVAEQQRAREMIAE
                     LRNRTPVVKESPRQWINPRPVTDKNPGNAVPGCPIIINTRQSITLQQVAQRISQNCHI
                     PVRITPDVQAYLSVTTSGSTQQIQGTVPPPDTGGMVPLAAIGSSDTTLPRLQGNTASL
                     PELKASGLTSLLNAVASRLGISWRYDGSHITFYYLDTRTFPVTYMDSQVAYNATVVSG
                     TMSSNGSSGGTASDAISGDASNTQTTTVDMKSALYQDVKNAVSSMLTPGIGRMFMSTG
                     FITVTDTPQVLETVRTFIEKRNEEMKRQVVLNVEILSIRKTRNEQAGIDWNAVFSDRT
                     LGLSLGSTFTSAASDTVTGGVSIVNGKLTGSKAFLKALSSQGDVSVVTRNSAVTKNLT
                     PVPMQIANQQSYIESVTTDTTANVGSSTSLNAATITTGFNMTLLPFILPDSQTLQLLY
                     SMSLSDKPVIENYESGGSKAQLPNVDLKTINQTVDLKSGQTVIISGFQQSGRRSGKQG
                     VGTPGFFGLGGGINSENDDTILVVLITPNII"
     gene            4334..5629
                     /gene="pilO2"
                     /locus_tag="KLK57_000006"
     CDS             4334..5629
                     /gene="pilO2"
                     /locus_tag="KLK57_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 4b pilus protein PilO2"
                     /protein_id="HBE0890502.1"
                     /translation="MTDISPTERQVFPLTAGRNVFLAGMDWKTLPASHKNPRTFARSL
                     GAVRYISCEYLSTEDTDRHIMVAAVSQNTIPKGSRRYFSLAMLILPLLEPGGYAIVEL
                     SQANDTELYGFVSAVDGILVSDLVGTREEIREAREIFLTINSAPEHGWTCYEPPSFNG
                     PDGRGPLPLETLTGAGKYPAEARLHPVSRGPQLIPVLLILTLLGTAWYGWQYYERLKA
                     EKAALAEAAQKAAEERITPPWLSMPETGDFIRGCSDVWSRLPVSVAGWRYSLAECAAT
                     GNSGNLRASYTNIAGTTVTAFSQRVREIFSIRPFMVMPDGNSGGFALPVTFSMPETPV
                     AVEALPENTLLQERLTTLARSMQLKMDWQEVSNSFTDEDGNTIQPPWKEYDLQILTTL
                     PAHQVAEHFSEPSVRFISVTRQLEEGRFRYQFTGKYYVQ"
     gene            5619..6077
                     /gene="pilP"
                     /locus_tag="KLK57_000007"
     CDS             5619..6077
                     /gene="pilP"
                     /locus_tag="KLK57_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis protein PilP"
                     /protein_id="HBE0890503.1"
                     /translation="MSSKTKALLLSVLILPTTVAASTISNMQQNNRQLQVEQIQEETA
                     LYEAMLAREKVRHELRTLEQGEPAEPVHSQESSSAVTTDTPAPITGIPRLTEISGVHP
                     QITARIQLPGGQTADVKEGQHIPGTTFRVEKITTDSVVLEQNGIRTTLQP"
     gene            6181..7689
                     /gene="tadA"
                     /locus_tag="KLK57_000008"
     CDS             6181..7689
                     /gene="tadA"
                     /locus_tag="KLK57_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HBE0890504.1"
                     /translation="MLAPADQPPDDILDYVVVDTDKTGQIHVRVVEGKKHLRAVQDYL
                     TRLCSRYPGKVSPFEFTTLDVIARLRQETVISGVENGINPVQQKMLGYISHAATLSAS
                     DLHITPGRDSTDFTYLEARVHGELELLDIVRKDEGLELLGATYSGMTDVIKGTQFDPG
                     VPQDARLAERFLKLAGLFGARYSHYPCVGGLYAVLRLIKDDSQHIPTFSMLGYHPEQE
                     RAVRRMLQRPEGIVILSGPTGSGKSTTLRTASAAYLEQYGFNDTGGILLPRRRLFTIE
                     SPPEGRIPGAIQTAVMDTAQGWVDSVKSALRLDPDAILNGEIRDHASAITAIKAAMTG
                     HLMLTTLHANDPINILERLEMEGVQARMIADPQLFIGLLSQRLVQVICPHCRLPWHEV
                     ESSRTDEERRLVENFCQPDAVYLRNHNGCPHCWRGVNGRTVIAEVISPDAKFFQIYRE
                     KGRIEAKTYWHRELGGMTRNQHLLGKINSGQVDPLAAHYISPVDEDSYTLLH"
     gene            7691..8785
                     /locus_tag="KLK57_000009"
     CDS             7691..8785
                     /locus_tag="KLK57_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HBE0890505.1"
                     /translation="MKILERLNNTELELSGLNRWLGKKTFRRTTFYESLAGMIRDGTP
                     VMRALEFICDVETDFGKKKGQSGLYFLATDCIASIRSSGQLSPALKDWVPKDEIALIR
                     NGEERGDIAEAMFQVVKTAKGRQEMISSLVSVCLYPLILLTLCVVNMYNVHTRVIPMI
                     SAFAPEERWSVQMVILAKSSGLVIGYGLWLLAGLLLLAILIRMSFSLYTGPRRRYLDR
                     IPPWSLYQTFHGVNYLFNISSMLQINIPLSHALTRIEKHAEHNRWLKSRISAIRKNVL
                     SGQSLALAMKNCGYDFPSKSCVNNLLLNSEREDSVASMALYADRWLEEAKKNVRKSGI
                     IITAISGLLVFLFIINMVSAIYGISDMLKQ"
     gene            8847..9383
                     /locus_tag="KLK57_000010"
     CDS             8847..9383
                     /locus_tag="KLK57_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003809694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBE0890506.1"
                     /translation="MSVITSSYSEHNIRHLQNDDKGMTLLEVLGVLVVAAIVIGAVMG
                     LMSDTLSSSDNQKELKNLQTIATKMKAQKFQGQYTGTDYVKILTESGGLPADMIAGGN
                     KAKNAWGGAVIIKVSSDKYSYVIESSNVPKKNCIDLVTSLRSSSMFTKINGNVTNKVD
                     PSTVCNADKTTIKLETNS"
     gene            9428..9913
                     /locus_tag="KLK57_000011"
     CDS             9428..9913
                     /locus_tag="KLK57_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HBE0890507.1"
                     /translation="MNRLFILTVIFSLLCSLPSAQASPQDKQYDRCFITAGKRYSIDP
                     GLLKIIARRESSLNPRAINHNRNDRGKIISTDYGIMQINSTHIPGLKKMGIIRSEKDL
                     LNNVCLNIQTGAWILARHFQRCGVNWECLGSYNAGFSKSNTHRRMKYARQIYSAYMQG
                     R"
     gene            9929..10555
                     /locus_tag="KLK57_000012"
     CDS             9929..10555
                     /locus_tag="KLK57_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HBE0890508.1"
                     /translation="MLQIIPALLVAITVVIQCLAVQMSRYRLSRMNARPPHHGLALMS
                     GMLAAAAIILRLYQNDSAVGVLFMTIMTGCMTLMTLTDAASCRLPRPFTIMYLISGSA
                     FRLWQGDWLTGLCSAGFWFLLLLLFRQYTSYRRKTETIGLGDVFLIAGIAIWSPPGDI
                     PWIVATAAGGALLYLWCNRQRRITRELPFGPFLCASQYAFTFYPGGLW"
     gene            10573..11934
                     /gene="pilV"
                     /locus_tag="KLK57_000013"
     CDS             10573..11934
                     /gene="pilV"
                     /locus_tag="KLK57_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shufflon system plasmid conjugative transfer
                     pilus tip adhesin PilV"
                     /protein_id="HBE0890509.1"
                     /translation="MQKDNDKGFALLEILGGLVVISLLMPLFWSYIEDYLNEMRNQSA
                     AFHADAYNTAARTYIADNNARLHSGTLPATFTADELIRKGYLKGLNRSPFGQSYTTGI
                     RRNTSTGRLEALTCSTGGENIKDDALRSIASLLPGLGGFIGKNGTATGVFGGWTDKPG
                     DYGLSCNGGHIAIVMMGDDLQESDRLYRFQVPGRPELNQMNTAINMGGNNLNNAGNVN
                     GQSATLKGDVTSENGWLITKNDKGWKNITYGGGFTMTDSQWIRAVGGKGIITTGEIKG
                     GKVSGGTVRSDGRLSTGEYLQLDKTAVANTKCSPDGLVGRDSKGAILSCQSGVWVGFE
                     SYPVGSPIPWPSATPPQGYLLMNGQSFSCSRYPQLARAYPSCKLPDLRGVFIRGWDNG
                     KGLDGDRNRQLLSYQADQSGVYHERGGWLKGHHSGMPYWAQGSTTEMRPKNIAFNYIV
                     KAS"
     gene            12407..13228
                     /locus_tag="KLK57_000014"
     CDS             12407..13228
                     /locus_tag="KLK57_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HBE0890510.1"
                     /translation="MKTDSTGIAARMMLSLDRERICECLLSHRQLQSTPLQVRYPQGV
                     RDALGIMSEQLSLSVSDLTRILVEDALSEMFLPADNIVRRLLSRMEHIMQAHDISATT
                     MAALLAPWNIRPAVFREPDRLTDYLTGEILAALADWFYLSPEWLNGRVHYPLYRPGDW
                     PATQEIFCRIISARENMDIILWHGFPFAGTHSGEYCGVLLRQKKEINNTIIYPVLSLY
                     PARMDIEKEGWFQMARKISPDIPVRAVTLTPAQAEYLITGKILPTALFRVPLSPW"
     gene            13330..14532
                     /locus_tag="KLK57_000015"
     CDS             13330..14532
                     /locus_tag="KLK57_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
                     /protein_id="HBE0890511.1"
                     /translation="MMMKKHITRTLIASAVLFSFNSTAATSYFEARNDAMGGTGVASS
                     HYGVAPLANPALLTKSGAKDDFSLLLPSVGAQLSDPGNIADNADKISDDWKAFDRAVD
                     SHSGVPQTAARLKERLQDFRNTHASAQAGVSAVAALPGDTLAAALMLKTLGTVSVDGK
                     VSDADLNYLESIADSGSQDVDKNRLTSQAFARAALITDVGVALATELETAGQKWSLGF
                     TPKFQRVDLFNYNTLIKNYDSSAFKGDRYHNTQNGINADIGASMDLDDNWTLGLVAQN
                     LIPRSIETKEVHGVTETFRIRPQVTTGVSWHNDMFTTALDVDLTPASGFTSDSKRQFA
                     AVGAEFNAWKWAQLRTGYRQNLASNNGSAFTAGIGLSPFDVIHIDVAGLVGTDNNYGA
                     IAQLQFTF"
     gene            14636..15094
                     /locus_tag="KLK57_000016"
     CDS             14636..15094
                     /locus_tag="KLK57_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238171.1"
                     /note="Members of this family include DotD of type IVB
                     secretion systems and TraH of plasmid conjugative plasmid
                     systems, both lipoproteins; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotD/TraH family lipoprotein"
                     /protein_id="HBE0890512.1"
                     /translation="MQKKRITLAGGVAMLCLSGCHLPPDNTAPARKYGAASEVAVTRH
                     AQYQLWEQGTYSAALPSRQPGGSLQAHSGRVSFDWEGDALELLSELARLRGQQFSYSG
                     VRLPLPVSLHVRDMTFADALRLTEAQTAWRAVLSQSAGRLHLSYMPTEKK"
     gene            15091..15927
                     /locus_tag="KLK57_000017"
     CDS             15091..15927
                     /locus_tag="KLK57_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000745049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HBE0890513.1"
                     /translation="MKKQVCLLLSGLLLAGCAGQHHRPPAGMAGNLPPPDLRAWLTPT
                     DNQDDGISDTRRQMLTDAGRTLGFRGGKAQRSWELIQALNARESTLNALYDFRPLISR
                     EGWLPPVIDEAQDVAHIQPDQIRTASRVWTILRPERFVSNPPGWRDWLLRGLSTTATP
                     GTEGRVVPEDRAQRRLWENALRQGWQEGRDNADLTLEANQKRLTRDYRGMMLYALLWR
                     QGMITRPDVTEQRQTVTGNGRKLITGDHVRRLKTHAEFTLQKSRWRPVVSTEGAPDEI
                     TR"
     gene            15911..17059
                     /gene="traJ"
                     /locus_tag="KLK57_000018"
     CDS             15911..17059
                     /gene="traJ"
                     /locus_tag="KLK57_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid transfer ATPase TraJ"
                     /protein_id="HBE0890514.1"
                     /translation="MKLPDEFGTFPFARFTAEEFRYFFAWCARHRVSDIDLTGGSPVS
                     VSRFGRRVRSSAHPLPSSIMMALSDELFGREVLPRVLSGHPVDRTIQVSGDASGRYGL
                     QRGQRVRLRCHLIQGTSATEEKVISVTMRVIPSDIPDILTMNIEPDLLDAMLRKTGLG
                     FICGETGSGKSTLAAALYRYIQTHFPDRKTVTYEDPVEYILGGENDLLPPHQAEIGRD
                     VESFAAGLRSAVRRNPDIIGIGEIRDYETADAAVQAGNTGHFCIGTMHTKSPGETFAR
                     LLGLFPPEIRDSMAAATLSLVQFILVQVLVRTNDGGRQAVREYIVITDELRDTLSRQS
                     HATWGHYIDEIIRKEKRRIRDQVLTMYQTGSIEASEAALFIPAGEFPK"
     gene            17056..17346
                     /gene="icmT"
                     /locus_tag="KLK57_000019"
     CDS             17056..17346
                     /gene="icmT"
                     /locus_tag="KLK57_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IcmT/TraK family protein"
                     /protein_id="HBE0890515.1"
                     /translation="MKREGFWRDVTPPLNIYGVPLPFTMIYLLLFPYPSKTTFWVCSG
                     ILLFFVLLGYSGWTVRTLCRRLYSLVQGKQASGRPWWYRHRTELPREWRGTE"
     gene            17410..21471
                     /locus_tag="KLK57_000020"
     CDS             17410..21471
                     /locus_tag="KLK57_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000842452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBE0890516.1"
                     /translation="MKSRYRTWLAVPPEETEAVKNAVPPLNGRKAVAWDPEKKLWYAR
                     AGTELSLLERWLPRPQELSMDAGDPVTEFAQVLENAGLVIQGLPQMDGAIHRVTTRDD
                     KKGAKSGAYRAYLDGRPAGWYRDYRSADDSPTNWVFSGGEQHDPLARLHLRAFAQQQR
                     DDNARKLQQQYNKQAGYARSYINRLPQATAHEYLTRKGIRAAPGVRLNNKNELVIPFS
                     NGRGEIRSYQRIPVTGGKDARILKDSEKTGNWFTFGTPENGRPLLFAEGYATAASLHE
                     ATGLPVLMTVDASNMIAVAENARQIWTDSPFVFCADNDHQREINKGVFSATKAAEVTN
                     GEVIIPAFTEAEKAQGLTDFNDLDASRGRDNFQNAMNAQLKHIGIPTPNSDTADHREA
                     VVIDNLIFTPVKDEKPQMSPENRQSTAPETELATQDTPYDTHSDDTENQAYEDDYAAY
                     ASLQSVTAETLSPDKPLENIASAADTGTENTSVPTAGDQEDLNTDATGTDTAHNSDTA
                     ETGTLPEPAGMQSVQQATTEPTAEDKPHENVVSAADTREENASVATADTGEENASATT
                     ADNQSTQAEEHPRSKDTPETSLSDAAGTQHTTTTQSEEQVQVSMASADDWSAFAEDLN
                     ADTPGAPTVHNTDNAETNTVSEPAGMRPVQQVTTELTAEDKAQEKSVASAAETGAEHS
                     SDITDDNRNAPAEELNADATVAVHNTGTTETASPSEPAETQPVRQATAEPTAEDKLQE
                     ERLVSATETSTANEAARPETPSLFSRLRRFLTGVKSRTSEPSVIQEKKPTEAETATEE
                     VAPPSPPETSPEDTSSAEPDTIIFAPRRPDSPQRHNLDEILKNLTHKEFPDRTALYSV
                     DGEPAFLDRLYCLEMVDGASADDKKVLAALAVATNFYGGVIELTGSDAFKQKAMQLIV
                     EYDIKVRMKLPAQRAALEKIRKEKEMAHDAIVTHRPTPELNRQSPEETTMAQPAPPVS
                     PKAEAEQTAGSTAETPHPEAPVTAKAEAPRADRPEQPAAPSSAPEKAPVSAQPQQPDT
                     PEEEPPGKLSPGESVTAVLTNYGEKEYAPGKGRCFFVELTNRSGKREYWGNGLEELVK
                     NHQKGDPVTLTLQARETFRLSGETRDRIRNIWAITPVSNGITVNNDQPHAGQHTLEFP
                     MATFSQVMLQMQQHWPQIMQEVRSPKNPPNNLYIGEDRQPASAPQDQSMRLPLAGNAP
                     NELIPAIASADKDNRQLNLLLVHSADDHLQGVVRLNGTLYPALATPSADNRQLVINAL
                     TDNGLQFAGYGEAVNHDENNHQRPSPQIMQFHLKQQDSPLFAAIHKPEEQPDKLFRSL
                     GFEQTWKEWSDSQKAEDRQEKTLQQAQSHSPGR"
     gene            21488..21838
                     /locus_tag="KLK57_000021"
     CDS             21488..21838
                     /locus_tag="KLK57_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HBE0890517.1"
                     /translation="MNMKKTLLVILTGITLASPVAGAATCRAGAAAEAGSRAAWERAR
                     TASQAWSQRENDVASSLQSCLSRIKDISITLPQFPSLDKLLRDLENQVCDAVVDRVND
                     HLPGNIDPWKDYNL"
     gene            21850..22545
                     /locus_tag="KLK57_000022"
     CDS             21850..22545
                     /locus_tag="KLK57_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotI/IcmL family type IV secretion protein"
                     /protein_id="HBE0890518.1"
                     /translation="MSEAQPTSPVSPEADNSGDIYNPVLRTIAQSEQRAALVPGLVRA
                     LLYSGAALCISLVANGIQYWHATGVTREYFATDNGRLVRLAPTSQPAWSQSDVMNFGS
                     MTLSEAFNLDFVHYRKQITTQAPRFSEAGFAGYVNALQASNILDTIKKEKFNLTTTIG
                     AGTLVNQGQMENGVWFWKFQYPVRMRLVGQTSTRPEQAFTFEITVRRVDPRIKPGGME
                     ISQMVSRNAAAGI"
     gene            22556..23530
                     /locus_tag="KLK57_000023"
     CDS             22556..23530
                     /locus_tag="KLK57_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001191879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraN"
                     /protein_id="HBE0890519.1"
                     /translation="MHIHHLIGALLIVCGTVTAADNPGWKNARSPQTGGKTAQATGNT
                     PAGGPPPAAQLPGNLPPPGAALVQEGASLDSPLNASEIRELRGIMADNERAISAPLTS
                     VVPRISSLTVNLSPGASLPLVRTALHNVSVVTFTDVNGSPWPQSDPPYNAAPGHFDVQ
                     FNGNMVSVTPLRAWAAGNVTVYLKGLSVPVILNITSGETDTTTLSQEIDSRLDLRIPR
                     RGPASPVVSIPSEKIALHDATLQAFLDGVPPDDPAIRRLKFSGNVPDTTIWQHGDDLL
                     VRSRAMLRDEFEQTLASADGTRLWKLPVTPLLTFSVNGQSVHVTPELE"
     gene            23534..24871
                     /gene="traO"
                     /locus_tag="KLK57_000024"
     CDS             23534..24871
                     /gene="traO"
                     /locus_tag="KLK57_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001272008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraO"
                     /protein_id="HBE0890520.1"
                     /translation="MSAEQEQDTAAKSGKKLSALLVMGGVIIIGGGYLLLTWLSPENA
                     ASLSSVNINSTTSGGGRNVMETQHYRDLLRADNERGAAEALRNNETFIASLPRGLDTD
                     TGNDQKRDNPPDDQSGTSGQNASPPQEDQTAREKRREALQKLLARINTQHQDARPPVV
                     AAAMWSTTGTPGTAPHASPSGTVTSQHPRLQETSLATPATRQGIQLIPALTRVPAYID
                     TEVDSDNAVSRVVASVPAGPWAGAVLYSQDARLAGQGMDIHFDRMMWRGMFLKVNAYA
                     LNERTGMSTVASDVNHRWFKHIVLPSVLGGVGNVGSLYKDANTQILQGNYGSITGRVG
                     MPDGEALAGVIAGGMAERGSQLLTRQAESEPYRQVTVSRGEVVSILFVEPVMSNDIST
                     PSSGTAPSESPSSGRPLTQAEAEHQASDRIRAAVLRKQNEMQQRYGIQQESYP"
     gene            24868..25581
                     /gene="traP"
                     /locus_tag="KLK57_000025"
     CDS             24868..25581
                     /gene="traP"
                     /locus_tag="KLK57_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HBE0890521.1"
                     /translation="MKNEHEDPGSFGETTAPASGKSFLKRQYMGYSLPVWAGCALIIL
                     VAIWYKFLRTPSEPPADAGFAVTPPAAESWAEPMTMTESGIGNNMPAPPVTPQADMQQ
                     LARDLKAELDRRDEEIRRTLTMLKDSITTLSNAIQKDEAWAQETRQLLEETRKQVHTL
                     QMQGALPAPTGNNRQPAKTLPSPLQGMKIMSVEKGMAWIRWQGSAWAVREGDSLGNIT
                     ITRIDPSSRAVITSAGTLR"
     gene            25581..26108
                     /gene="traQ"
                     /locus_tag="KLK57_000026"
     CDS             25581..26108
                     /gene="traQ"
                     /locus_tag="KLK57_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraQ"
                     /protein_id="HBE0890522.1"
                     /translation="MSIDALTAIENFASGIFSAGMEFLFTWGELLGIIGLIGHLMRAR
                     AEGRHSMGPGKFIAGIFICGMLVSLPSMINAGGTQMGFRADSFAPIAYVQPGSFGAAA
                     GAVNAILSLAKLAGVGFVMNGISIWRKSLLDGHTALSASESISKGNVKFVAGVLLVFN
                     DRVIDATLASLGIAF"
     gene            26155..26553
                     /locus_tag="KLK57_000027"
     CDS             26155..26553
                     /locus_tag="KLK57_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001605438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HBE0890523.1"
                     /translation="MSRLRAMAARLRAASVYVQLCLMACRDRLVRLLLLLPVMLLPHT
                     AMANTLFDMIKTVGDGARGSSSSFVAIGQVIGLVLFIGGLLGLKQIGKKPGAGLACLL
                     SILVGVCLAVVPELMGSGQKTLGLSPVSIS"
     gene            26610..26861
                     /locus_tag="KLK57_000028"
     CDS             26610..26861
                     /locus_tag="KLK57_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003665769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraS"
                     /protein_id="HBE0890524.1"
                     /translation="MSCELPPFTLLRTEHGNVIARDNRDNQEFVFAECYSTAIAALIL
                     AAVTLYTERKAYEKDPATHRPGPGTMAFPAPATGHGIQR"
     gene            26776..27594
                     /locus_tag="KLK57_000029"
     CDS             26776..27594
                     /locus_tag="KLK57_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraT"
                     /protein_id="HBE0890525.1"
                     /translation="MKKTPPLIAPVRERWLFPLPQPDTVFSGEALLPPPVLSTEGRCP
                     CCRRTVTHRFILEDSWPLQQMADTCRDTIVLLEKNLTRVMRLKKRPVPENADEKKKHT
                     RTLQDAERSLAQARLSARRLALRHVEKSQIVTTDALSENESDLLQPEGPPFHLCAFCH
                     AWHCLNGYAAAQGVMVWLPDLHPASVVALNARALQEIFSDNRQRVRQGRAVLNALVQN
                     RLAVEEKFRTWRPADFADALRRWPPAQRKTLREKMDGVALILLPDSFPDKKYVM"
     gene            27889..30933
                     /locus_tag="KLK57_000030"
     CDS             27889..30933
                     /locus_tag="KLK57_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE0890526.1"
                     /translation="MNINRIIFAIEDCISTLSRYSVSAFFPDYCDLHTVIGLDQQDRE
                     RRPWLKAPYIATTKQGYYLSVFEISGALKEMDENPDQTAPGTLESLIAALAERMNTAW
                     KNSGHKISFVFERDPERGRDEIEAMLLPQRKSIARTGILLQDVLDEKVTTLTPWLVRE
                     RCWLTVWSSEELLSRTDRKNHQTRVRKLAEHAPPARFAQDPWRWTLSALKIRHDALLD
                     TLEQALTHDSDGLLIRLMDIHEVGREIRRQLERNSTPAVWQPHLPEDARPAGWRQGGD
                     TSVFHAPSLNLQLFTTQPETHGSLIQAGELWHGMVAITLPPQNLRTFNQLVRDVPRAI
                     PWRIRMDLMPDGMKALGVKKTLLTYSSFIPPLRPMYDSVMMLNDINKHDPVCIMTIVA
                     TTWGNSREVCTRNQALLKSALEGWGVCGTTTTFGDPRRAWVNTVLAASHSSGPIPLYP
                     PLSHALSLFPLNRAGSVWRGQGNLMMHTEDGSAWEVALASSQQNKHTELTPGAPGLGK
                     SVLINALSEIQIASAQKNLPFIAYIDKGFSAQGLVQLIRDSLPEQRKDEAVGIILSND
                     PDHTRNLFDVMYGARKPVTPEKNFMVSVLCALCVDTGTGQPCNPGDTRQIISSLVDLA
                     FREYGENNPRLYRAGTEPLVDLALEESGIAEQHDAGWWNAATWFEIRDMLHIAGNIPA
                     AQRAHYQAMPLLAEMSALLGQPSIRDVFGTVQRDNSEERLLDYIRRALDQGHSDYPMM
                     SGCTRFMLSPDTRVVAVDLNNVAGDKTPAGRLRTGIMYLLAGQIAGGDFVLPQYQEEI
                     RKNLDPRYHEVIFRRIEQLDQEVKTKVYDELHNAKGINFIWEALDTQELEQRKFGIRT
                     VLSTQLLQHYPPAVLKSANTLWLLRYRPDEIPFLRDNFGVPEVTLRRFLKMPEGAAPD
                     GSGVPVLAVFRVKNGTLARILKFTLGPLELWALNSSPKDSALRRALTQEVGSLRARQI
                     LAEHFPRGSATSLIEHRARTHDSENVIHELAAELIRKQGYNL"
     gene            30933..31553
                     /locus_tag="KLK57_000031"
     CDS             30933..31553
                     /locus_tag="KLK57_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraV"
                     /protein_id="HBE0890527.1"
                     /translation="MYSIHITPRCRRPARGQVFLKTLRVDGDSFSFTLPSACFTLRPS
                     PNPRVIFASDRRADGEMSGIRLALPGETGTLITTATWQVNVPERPPFDIQHVIHWLLP
                     APLTALMSDNTWLWPDVPGTPALTAGDWPVMESDTPTLKRVKRRTFTLIDNRNRQGWI
                     TRRFQCFPLAPLPESLSHRVPDILYPPKTEKKECKDNLQPHCLHSL"
     gene            31511..32716
                     /gene="traW"
                     /locus_tag="KLK57_000032"
     CDS             31511..32716
                     /gene="traW"
                     /locus_tag="KLK57_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001387490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraW"
                     /protein_id="HBE0890528.1"
                     /translation="MQRQSATTLLALLVGCAVIPATHAYSVNVVSSQPVTRQIMPELA
                     TIQGIMEEMVRMQTATGTAITQNSEKLATVIAQDGQATRQQMIFSNETHRLEEARKSF
                     SVPDSICSESASGIAAESRRAAASAAARLSQGGGVSSKPIRERLSRAADSPVREAYDS
                     AGIHAGYCTEAEYARFGGTDVCPAVGDLPGGDSQVRSLYQGAGTADTPAALTWDQKQI
                     DAATAYMKNTARPSAGRAPGKGEVGTQTGRTYVGLQNEYNGIIDAASHPQLSLIADST
                     PNEATRGALTEALQSPSAAAYFDRTASSEARTRGHMSQREFEAFEAGRRYANTDWQQD
                     LQGMEGDNLLRELLRTTALLNWQMNDLKEQIRQGNVIAGQQLALAARQYYGQRLGELS
                     QAMGQGSVR"
     gene            32713..33282
                     /gene="traX"
                     /locus_tag="KLK57_000033"
     CDS             32713..33282
                     /gene="traX"
                     /locus_tag="KLK57_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000650756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraX"
                     /protein_id="HBE0890529.1"
                     /translation="MKDDNSIDEKKPAPRLRRIGDIGINLFAPLAETRWMLRSCRHML
                     DQHKTRLKRLMPDRQPESPVCMTWEEAVRASGLTVDELDRRLRRRRMFWRTGFWLMMI
                     PVLLLCGMALAATALPVITLIRILVCLLLMSTIAALCASKALIASYRLWQLNERRVSE
                     DEQGTFPDYLQARHGWKQATWLAITHRQY"
     gene            33357..35522
                     /locus_tag="KLK57_000034"
     CDS             33357..35522
                     /locus_tag="KLK57_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DotA/TraY family protein"
                     /protein_id="HBE0890530.1"
                     /translation="MKIIPRALSAGLALAAVPAQAFVTYQDIVSAATNPDDLSRQALV
                     TIFGDVVTNPLTASSNTIIGNLFMVFNAIIATMGIVWFLFIGIRHAVRTGHQGSVFNN
                     GRDVVGILSVVSGFLMIVPTASGWSLAQLVMLWGASIMGIGSANIMVQTAAENIAEGY
                     SMTVQPVHVSTRTAARGILEMELCKHAMNTGLNDFNRTARSSTPPMSESSRTENGRYT
                     VTVSNGSATCGSVSLSVKGNGSNKQSALAHFFNPFIETEYQGVLTAQRSAMDTMLRDL
                     DSAAQEFVSAYRTRRDTGNGNLPDIESRIQKAANRYEETVQKALPTDNAETERREQLK
                     SYLTTYGWVALGAWYQTFATANQRLAELADRAPTVSGVSSLGEAGDTEFLKEVKGAYQ
                     AQLQNSAYTPALGTTTSASEYHASNTQNPQSALLEFIGPGLTRFSNKVATIHSGTGTT
                     SQQANPLLKMKDIGDYTMVTTETIWAGYTTVRVLSTAGSNSVFGRLVNAVSGLSDAAK
                     AFLEASAPPLYFLLFLLFCAGFSLSIYLPFIPFIFWMTGIGNWIVSVLIGCAAGPLWA
                     ATHLGTSQDRGSRAAYGYIYLIDSMIRPLLMVFGFFFASVAIVAGGTILNALFAAALM
                     NVQINSVTGVFSMAGFLMIYARACTTLVAAIFALQAYLPDHVINFLGGRDGVNMLGNM
                     VSSVKDIIAGGNTNMRRTPGVREDRLKNINRDDKDGIKQ"
     gene            35611..36261
                     /gene="excA"
                     /locus_tag="KLK57_000035"
     CDS             35611..36261
                     /gene="excA"
                     /locus_tag="KLK57_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid IncI1-type surface exclusion protein
                     ExcA"
                     /protein_id="HBE0890531.1"
                     /translation="MNKTKSRYPSWWAYYHLARNAYFLLGIPCLLLYSAAYTSDLLDD
                     NLRHSISLFILAYGCYFLLFIPPLWLYLRTRAKKKKVQQVVQQIKESSPFAPTSDYEQ
                     LSFSKSCYFGIDIKNGTMLYVRIYPNNVMDVIGLDIHNFTRTVAEDGKLEIHTTYVSL
                     PMIPLEISGISARTLANTMHTMAARGYEYKERFPQMIRYRVKEWEKVAGVPVAEVF"
     gene            36535..38121
                     /locus_tag="KLK57_000036"
     CDS             36535..38121
                     /locus_tag="KLK57_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001506542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TnsA endonuclease N-terminal domain-containing
                     protein"
                     /protein_id="HBE0890532.1"
                     /translation="MSESRSNFPARHGTPWNTEELQRLLVSIAEGVSLKKLTQTHQRS
                     SGGIRGAIKRLLPSEFLEFNDINTVNDLSNYFNKIPRQEHNSIINNLISLSTAKTIAH
                     DKVEIQKNSFKQNTPLPLCQPNTDFKDYIADEHDVIMLIHSAIESLSNKRHQYILLMR
                     LGTDDSPHSLADIAVKLGISRERVRQLQERALGLLAAKSRYEGTPGACLKALINSIGN
                     SPYDIAVWIYRIVHRDFVTSSELAAKFIIFAAGIPKARRDEIKNSLSLISQLQKQKLR
                     ERRAELAHAHAQERARIKKERVEFIFSKWLDNTHWPKYIAPPPPVEQLCSQRAVNDTD
                     ISGFFYSQKLGRTVQYESGLEFKIMNLLECCDQVLFYQEQPFSIPYCFKNKSKKYYPD
                     LLIATVDGRCLLMEVKPTDRMTLSLTRIKSNAGRKWAHTRGWGWLVISDRFSLRQLEE
                     HAISTNTWKLIEAELKTSRILAWREMMELRTRYEIHRLDLIAYIIQSGAEVDNFYRIT
                     PKISNNTSNQRQPNESDCHN"
     gene            complement(38259..38411)
                     /locus_tag="KLK57_000037"
     CDS             complement(38259..38411)
                     /locus_tag="KLK57_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001618166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hok/Gef family protein"
                     /protein_id="HBE0890533.1"
                     /translation="MPQRTFLMMLIVVCVTILCFVWMVRDSLCGFRIEQGNTVLVATL
                     AYEVKR"
     gene            38741..38983
                     /locus_tag="KLK57_000038"
     CDS             38741..38983
                     /locus_tag="KLK57_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890534.1"
                     /translation="MKDEIHSTSINPDVVEQFLALMNTHCKSSEEALAMLSTLCYNLM
                     MTCGIHSMDSQMLNGHGISMQLQGIDIAERPGKNIH"
     gene            39282..39650
                     /locus_tag="KLK57_000039"
     CDS             39282..39650
                     /locus_tag="KLK57_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001456887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890535.1"
                     /translation="MKDNNTTATPEAWKMRFLANGTSGTWFYTDDIAVTDAWLRAHPD
                     DVEVIPLCRIQPRQHSHEQHIEELRAALARMLHMHHMMLKTTHYRENITDARCIREMH
                     EAPLQALNILSRYPHKKDEV"
     gene            39654..39869
                     /locus_tag="KLK57_000040"
     CDS             39654..39869
                     /locus_tag="KLK57_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890536.1"
                     /translation="MWLLIFWLSSPFNVSSGDARQPTLAVHSQAFHTERACRDALRAI
                     KKENVGEFSLRGVCVSDGNTKEEAESS"
     gene            complement(40067..40690)
                     /locus_tag="KLK57_000041"
     CDS             complement(40067..40690)
                     /locus_tag="KLK57_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001466373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE0890537.1"
                     /translation="MQEHIDYQIEKYQFRARNESPRLMRQWEDVMRECRDTGAGARER
                     LRIALLNVDYVTSFELPFRLLLTRTPQMIDSVRREWQLSQKKVVFNERQRGCVYSLQN
                     DLSGVPDSFSYSLATRIRRVDSYGMTTRCFTDIAGQVKAPTARLKLALESGLIVTALD
                     GLFWLGIQRIAADVQRLRQSGMTIITRDVEVSDSLTGTTRGVSGYSL"
     gene            40849..41316
                     /locus_tag="KLK57_000042"
     CDS             40849..41316
                     /locus_tag="KLK57_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000598960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBE0890538.1"
                     /translation="MIKYIFSLTIIFSASLCAAQIQGHVIRVLDGDTLEILQDKKPVR
                     VRLANIDAPEKKQPFGRWSATQLKDLVAGKPVTVQYAQTDRYGRILGRVYTADGTEAN
                     RWQVRNGAAWVYPDYNNDYTLPPLQRDARALKRGLWADKNPVPPWTWRKQNKK"
     gene            41468..41644
                     /locus_tag="KLK57_000043"
     CDS             41468..41644
                     /locus_tag="KLK57_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001054900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890539.1"
                     /translation="MNLVDAFVTKVISGPYEEYGKWWVDVEYISWGVPGKSRLMFDSR
                     EQAQEVQEGFRFLT"
     gene            complement(41707..41958)
                     /locus_tag="KLK57_000044"
     CDS             complement(41707..41958)
                     /locus_tag="KLK57_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890540.1"
                     /translation="MNRLQVLAKTSGGAGFGALAGEELSITDTIENVATGNVRANHGV
                     LSPLMEKRMINLLIIVLRAVVAVANAVIALLELIRELVD"
     gene            42570..43592
                     /gene="istA"
                     /locus_tag="KLK57_000045"
                     /pseudo
     CDS             42570..43592
                     /gene="istA"
                     /locus_tag="KLK57_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
     gene            43589..44371
                     /gene="istB"
                     /locus_tag="KLK57_000046"
     CDS             43589..44371
                     /gene="istB"
                     /locus_tag="KLK57_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HBE0890541.1"
                     /translation="MMMELQHQRLMVLAGQLQLESLISAAPALSQQAVDQEWSYMDFL
                     EHLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIER
                     NENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQ
                     RGVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGD
                     AALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
     gene            45003..46310
                     /gene="trbA"
                     /locus_tag="KLK57_000047"
     CDS             45003..46310
                     /gene="trbA"
                     /locus_tag="KLK57_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001390885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IncI1-type conjugal transfer protein TrbA"
                     /protein_id="HBE0890542.1"
                     /translation="MSYQQTSAAEDPMAIWYIVGAICLLFAIIIWRFLPEIVFASCLI
                     LHTLWGMIDWGPFHNFAAPRYNLLAITANNAATITFSQWLDVMSRTVGILWLILLPMT
                     FGFLWMWFHHPAQPRFTRRPLNIHTLPHLFSSLSPAIAPVLADGDSNRLFHGQKRPER
                     RVALTPEAFVEQNNLIRNMQLDVAATRQCFMAQLGQPLTTWKDMAPHEKALFAVFGLQ
                     FFLGDRKSAVSLMNNLNLSCRLKSKRDQGRFSTPVYSLARNAFIRVIKTEGAQQWLRQ
                     HRYVRSGLVWLYAHDLRLTPPNWLWLKGVDRTLFYALHRANTTKGFIEGAGVVAVARA
                     ENEASRLGLPCPEPCVEEAIEGLRRDMLGLGLIWDEPQPDRDRKRQIRTRWSLTDDVI
                     PRRHDNDEDTDTGETTETTETTETTETTETTEKQHPADKEKEQ"
     gene            46307..47431
                     /locus_tag="KLK57_000048"
     CDS             46307..47431
                     /locus_tag="KLK57_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004031782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HBE0890543.1"
                     /translation="MTIHYTATLNNKHLSLIALYPDGNREVIAILPVTELGALINRNN
                     RLMHLSFSSPFREITETVLVDTVDAERPGHHIGRMLSAIDACLDAEYAGKGKHPSRKN
                     GFWRTLMCGFIAGIALPATVAGYHWMKAEPPSTLAYPTSQEWRAEPVTDTETPRPHTE
                     HHVNSIPARPAPTQQEPSSAPAPTAVTEEQHVQAAEARRNLATVLKRNADRGMFTINL
                     STGHERTLYAFLDPACINCRLLEPALKQLAREFSVVIYPVSVIGGESSTKRTAPLLCE
                     PDSQKRATGWHQLYSADSGMAPTGDAPAAQDGQCLTAARAAIDVNNQAFRQFGFGGTP
                     WVLSDTGWHLPSGILKDATSVRLFLKATDSEEYHNEPAPR"
     gene            47412..49715
                     /locus_tag="KLK57_000049"
     CDS             47412..49715
                     /locus_tag="KLK57_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HBE0890544.1"
                     /translation="MSQHHVNPDLIHRTAWGNPLWNALHNLNITGLCLAGSIITALIW
                     PLALPVCLLFTLVTSVIFMLQRWRCPLRMPMTLSLDDPSQDRKVRRSLFSFWPTLFQY
                     EADETFPARGIFYVGYRRINDIGRELWLSMDDLTRHVMFFATTGGGKTETTFAWLLNP
                     LCWGRGFTFVDGKAQNDTTRTIWYLSRRFGREDDIEVINFMNGGKSRSEIIQSGEKSR
                     PQSNTWNPFAFSTEAFTAETMQSMLPQNVQGGEWQSRAIAMNKALVFGTKFWCVREGK
                     TMSLQMLREHMTLEGMAKLYCRGLDDQWPEEAIAPLRNYLQDVPGFDMSLVRTPSAWT
                     EEPRKQHAYLSGQFSETFTTFAETFGDVFAADAGDIDIRDSIHSDRILIVMIPALDTS
                     AHTTSALGRMFVTQQSMLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYT
                     DRIAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTA
                     RTVKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFT
                     VFQGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPE
                     HVSSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANE
                     LWKKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPD
                     LTASPPHKKNVAQTPSGGKQESRKKRF"
     gene            50579..51394
                     /gene="sul2"
                     /locus_tag="KLK57_000050"
     CDS             50579..51394
                     /gene="sul2"
                     /locus_tag="KLK57_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBE0890545.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            51455..52258
                     /locus_tag="KLK57_000051"
                     /note="aph(3'')-Ib"
     CDS             51455..52258
                     /locus_tag="KLK57_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBE0890546.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            52258..53094
                     /locus_tag="KLK57_000052"
     CDS             52258..53094
                     /locus_tag="KLK57_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBE0890547.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(53066..53206)
                     /locus_tag="KLK57_000053"
                     /pseudo
     CDS             complement(53066..53206)
                     /locus_tag="KLK57_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(53201..53470)
                     /locus_tag="KLK57_000054"
                     /pseudo
     CDS             complement(53201..53470)
                     /locus_tag="KLK57_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(53700..54131)
                     /locus_tag="KLK57_000055"
     CDS             complement(53700..54131)
                     /locus_tag="KLK57_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HBE0890548.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(54199..56910)
                     /locus_tag="KLK57_000056"
     CDS             complement(54199..56910)
                     /locus_tag="KLK57_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBE0890549.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVELGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(56922..57254)
                     /locus_tag="KLK57_000057"
     CDS             complement(56922..57254)
                     /locus_tag="KLK57_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBE0890550.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            57487..57822
                     /locus_tag="KLK57_000058"
     CDS             57487..57822
                     /locus_tag="KLK57_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HBE0890551.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLTDVRDTLKNAGYEAGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(57908..58756)
                     /locus_tag="KLK57_000059"
     CDS             complement(57908..58756)
                     /locus_tag="KLK57_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBE0890552.1"
                     /translation="MNQTLPTADLNTAGTTDVIPSVAIDRIIAQRNEGIALFMQAMEC
                     LATARKILLDASGDIFLYGFEDCVTDSVRRMDKTEEAKRNITRLADRKIWDRLMTDTG
                     MYTFMSSCQRDEWNSQLMSDTCPEITLDNVLATFRHLNASKMQTFEQGLIDVYRKLSW
                     DYRTNNPCRLGKKIIIENLLYRWSNGRVTLDCSGREALDDLVRPFYLLEGRNVPDFRN
                     SIGAQYGEFLGNGDNVGKLLEGEYFTVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPG
                     ALPPRV"
     gene            59336..59587
                     /locus_tag="KLK57_000060"
     CDS             59336..59587
                     /locus_tag="KLK57_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003980153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0890553.1"
                     /translation="MTGWELRIWRKSMLWSREKAAREFGVTQRTWHAWENAEQVDVTV
                     WRTTQALSVRDLLPHMQGMRKADIIRRLENELGETAGNV"
     gene            complement(59618..59863)
                     /locus_tag="KLK57_000061"
     CDS             complement(59618..59863)
                     /locus_tag="KLK57_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014358130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890554.1"
                     /translation="MRTTKPGKMPGFFVSRSRANSMTIAERLIQKGFDEGFKQGALEV
                     AREIACRLRDIGWPLERIQEVTGLSGEELKKLFPDEQ"
     gene            complement(59863..60789)
                     /locus_tag="KLK57_000062"
     CDS             complement(59863..60789)
                     /locus_tag="KLK57_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBE0890555.1"
                     /translation="MSKKKNTTTPTPHDAAFRSFLANPDVARDFLELHLPAEYRQLCD
                     LSTLKLEPATFVEPDLHQYASDILWSVKTTGGEDGYVYTLIEHQSTENLYMPFRMLRY
                     SVAAMQRHLEQHKTLPLVIPVLFYHGERSPYPYSMNWLDCFENPALAAKIYTKPFPLV
                     DITVVDDNEIMNHRRMAALTLLMKHIRHRDMMELLDKLPQVMVEISDEQVRVLIHYIV
                     NAGDSVSPEFMRALAERLPQHEDKLMTIAERLEQKGRQEGRMEGRMEGRMEGALEKAL
                     AIACQLQKMGMTPEQIKQATGLSDDELKKITH"
     gene            complement(60786..61175)
                     /locus_tag="KLK57_000063"
     CDS             complement(60786..61175)
                     /locus_tag="KLK57_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CbiX"
                     /protein_id="HBE0890556.1"
                     /translation="MKSCFDTDKAFHMSFDEVTFNADRIRSKLELLSEELMPRCGLVY
                     ELYQRRLSAAIDNFVATLPTEQHLLVLELAREEFDYLSAEEIAEEIRRDSERGYCCHG
                     FERNCCPLGCGDLGDDCCDAADLMEEP"
     gene            complement(61353..61619)
                     /locus_tag="KLK57_000064"
     CDS             complement(61353..61619)
                     /locus_tag="KLK57_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890557.1"
                     /translation="MRPSIIFATAEYVKRLREECLRENKPLHRHTRFRRQELAPDEIN
                     PDVLAMGGHIARRCSEQKRVRIPAMKVSEWGHLLRALEIERVCH"
     gene            complement(61712..62146)
                     /locus_tag="KLK57_000065"
     CDS             complement(61712..62146)
                     /locus_tag="KLK57_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="post-segregation killing protein PndC"
                     /protein_id="HBE0890558.1"
                     /translation="MTVSSTISVFCRDGVFRTVYCHLHGEPTWNGRILHTHYATGQQA
                     EALVEHGDIRCLGPRCDKPAGHTLQNPVDGVTAYYGRDSGFRMDSEAREYRSFREAIA
                     TESTEEVRFHYVFIDGYWKVMYRTPEGWKMKALALALRRCPE"
     gene            complement(62879..63379)
                     /locus_tag="KLK57_000066"
     CDS             complement(62879..63379)
                     /locus_tag="KLK57_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HBE0890559.1"
                     /translation="MSVVAPAVYVGTWHKYNCGSIAGRWFDLTTFDDERDFFAACRAL
                     HQDETDPELMFQDYEGFPGNMASECHINWAWVEGFRQARDEGCEEAYRLWVEDTGETD
                     FDTFRDAWWGEADSEEAFAVEFASDTGLLADVPETVALYFDYEAYARDLFLDSFTFID
                     GHVFRR"
     gene            complement(63841..64437)
                     /locus_tag="KLK57_000067"
     CDS             complement(63841..64437)
                     /locus_tag="KLK57_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000978013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890560.1"
                     /translation="MMKSDEKYQVPAWMRPLLPLLCNTGGNDPEELLNDTETTASANV
                     VRYVLIVAVRSQVDLLQLLYRKGLLRTEIPGGFSPEEAQALLDNLVRSHISKALSGER
                     MAARDRNADLAWIRQQLVDAAWFVRATLEAHGMSVGNESPPAPPETMPDIQTRELVML
                     IKRLASSLKAVKPDSCVVREAQDWLRDRKLVDITDILR"
     gene            complement(64434..65153)
                     /locus_tag="KLK57_000068"
     CDS             complement(64434..65153)
                     /locus_tag="KLK57_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBE0890561.1"
                     /translation="MSARSRALIPLSAEQQAAMQAVAVTEQRRRQGRTLSAWPYATAF
                     FRCLNGSRRISLTDLRFFAPALTKEEFHGNRLLWLAAVDKLIESFGEVCVLPLPSDAG
                     HRLFPSVPFREGERRRQKTTLTEQKYSRQREREAERRELEYQTCFAQAQIDLAFHTPA
                     TVGSWLSRWSGVVEEHDLETIFWGWCGRFPSLSSFDRFFWQEEPLWRLIFEAGEAGRG
                     APVQVRALEQWMIPNKLENVI"
     gene            complement(65150..65584)
                     /gene="psiB"
                     /locus_tag="KLK57_000069"
     CDS             complement(65150..65584)
                     /gene="psiB"
                     /locus_tag="KLK57_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBE0890562.1"
                     /translation="MNTELTLNALQSMNAQEYEEIRAAGSDMRRNLTHEVMREVDAPA
                     NWMMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQLWMLVLVNGGGQPFAVV
                     QVQHIFTPVAISHTLALAATLDAQGYSVNDIIHILMAEGGQA"
     gene            complement(65639..67597)
                     /locus_tag="KLK57_000070"
     CDS             complement(65639..67597)
                     /locus_tag="KLK57_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000117326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBE0890563.1"
                     /translation="MPVTKCEPETTRKASRKSVKTQETALSALLAQTEEVSVPLDSLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRYGVAAGGRRLAALNMLA
                     ERNILPADWPVRVKVIPQELATAASMTENGHRRDMHPAEQIAGFRAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDRITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPDVRVIRNLITESEVAVAENSKFRFVGADAFSPDELRTDLFSDDGDGYVDRV
                     ALDAALLEKLQAVAEHLREAEGWEWCAGRMEPVGECREDAGTYRCLPEPEAVLTEAEE
                     ERLNELMTRYDALENQCEESDLLEAEMKLMRCMAKVRAWTPEMRTGSGVVVSWRYGNV
                     CVQRGVQLRSEDDAADDADRTEQVQEKASVEEISLPLLTKMSSERTLAVQAALMQQPD
                     KSLALLAWTLCLNVFDSGAYSKPAQISLECKHYSLTSDAPSGKEGAAFLALMAEKARL
                     AALLPEGWSRDMTTFLSLSQEVLLSLLSFCTACSLNGVQTRECGRTSRSPLDSLESAI
                     GFHMRDWWQPTKANFFGHLKKPQIIAALNDAGLSGAARDAEKMKKGDAAEHAEFHMKD
                     NRWVPGWMCSPRPQTDATERADNLADAA"
     gene            complement(67656..67889)
                     /locus_tag="KLK57_000071"
     CDS             complement(67656..67889)
                     /locus_tag="KLK57_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 domain-containing protein"
                     /protein_id="HBE0890564.1"
                     /translation="MSEYFRILQGLPDGSFTREQAEAVAAQYRNVFIEDDQGTHFRLV
                     VRQDGTLIWRSWNFEDGAGYWMNQYIRDFGILK"
     gene            complement(67947..68474)
                     /locus_tag="KLK57_000072"
     CDS             complement(67947..68474)
                     /locus_tag="KLK57_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE0890565.1"
                     /translation="MSARGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSESWRDK
                     QTGEMREQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTVQMLGRAPQQNAQAQPKPQQNGQPQSADATKKGGAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            complement(68858..69172)
                     /locus_tag="KLK57_000073"
     CDS             complement(68858..69172)
                     /locus_tag="KLK57_000073"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890566.1"
                     /translation="MRGAAPAALPARLSGQKVVYGCMQGTGVIFRDTVTVSGCLLLRP
                     LRLPAAWRHPVVRPDSDRQLLAHPPVSAEPAGAVSGCRYGPMRENPACSDRSGRAAPV
                     AP"
     gene            complement(69243..69434)
                     /locus_tag="KLK57_000074"
     CDS             complement(69243..69434)
                     /locus_tag="KLK57_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890567.1"
                     /translation="MNISTETREILRNYRAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSRNR"
     gene            complement(69431..69853)
                     /locus_tag="KLK57_000075"
     CDS             complement(69431..69853)
                     /locus_tag="KLK57_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBE0890568.1"
                     /translation="MYCTVKEIIREVLDTDVPDSECVFAVVLTRGDVRHIARDWSLSD
                     DELETVMQRLDDAFVYGACDRVVSDIVNELMEEKRASRHVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREELEAMDVVLQALDGETMS"
     gene            complement(69900..70325)
                     /locus_tag="KLK57_000076"
     CDS             complement(69900..70325)
                     /locus_tag="KLK57_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBE0890569.1"
                     /translation="MQYAKPVTLNVEECDRLFFLPYLFGQDFLYVEASVCALAKKMMP
                     EYEGGFWHFIRLPDGGGYMMPDGDRFHMVNGENWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTHLYRMRDAQLWSHIEFHPECNAIYAALD"
     gene            complement(70740..71426)
                     /locus_tag="KLK57_000077"
     CDS             complement(70740..71426)
                     /locus_tag="KLK57_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890570.1"
                     /translation="MNETLNALICRHARNLLDAPRLATLLINRHGGVLPPLLASAIQK
                     LTGTGAELVLSGSQWQSLPVLPADGTQVSFPYAGEWLTEDEIRAVLDAVHDAVRSVSC
                     RVAEDARRIRAALTTTGQTLLTRQTRRFRLVVKESDHPCWLDEDDENLPVVLDAILNR
                     GARFSSVEMYLVSECVEHILSSGLACDVLRIPDEPSRRWFDRDILREVVLEARTEIRS
                     MADALAKIRK"
     gene            complement(71471..71905)
                     /locus_tag="KLK57_000078"
     CDS             complement(71471..71905)
                     /locus_tag="KLK57_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBE0890571.1"
                     /translation="MYGTCETLCRALAAKYPGDTPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGAAMEIINNVRENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDNGLAVPECVTRRQAVVNQARALLKNNTREND"
     gene            complement(71919..72140)
                     /locus_tag="KLK57_000079"
     CDS             complement(71919..72140)
                     /locus_tag="KLK57_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890572.1"
                     /translation="MNYAGHEKLRADVAEVANTMCDLRARLNDMEHRCRFDSDVLVER
                     LTRQTLYRANRLFMEAYTEILELDSCFKD"
     gene            complement(72141..72824)
                     /locus_tag="KLK57_000080"
     CDS             complement(72141..72824)
                     /locus_tag="KLK57_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBE0890573.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLTAVQRAMQQGAAN
                     DNWFEPEAA"
     gene            complement(72900..73211)
                     /locus_tag="KLK57_000081"
     CDS             complement(72900..73211)
                     /locus_tag="KLK57_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890574.1"
                     /translation="MKYDGDGRATARFFSGKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RWARDGRFYRSRLAPVTVYASLSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            complement(73208..74134)
                     /locus_tag="KLK57_000082"
     CDS             complement(73208..74134)
                     /locus_tag="KLK57_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HBE0890575.1"
                     /translation="MPNWCSNRMYFSGEPAQIAEIKRLASGAVTPLYRRATNEGIQLF
                     LAGSAGLLQIAENIRSEQCPGVTAAGRGAVSPENIAFTRWLTHLQNGVLLDEQNCLML
                     HELWLQSGTGQRRWEGLPDDVRDTITALFTAKRGDWCGFWSNEDVSVWWNRLCDNVLP
                     EKTMPFDLLTVLPTRLDIEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQG
                     ENFIQVDFDTPWCQPESDVVAELSRLFGCTLEHWYAEQGCDFCGWQLYERGELVDVLW
                     GELEWSSPTDDDELPEVTGPAWIVDNVAHYGG"
     gene            74529..74777
                     /locus_tag="KLK57_000083"
     CDS             74529..74777
                     /locus_tag="KLK57_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBE0890576.1"
                     /translation="MIRIEILFDRQSTKNLKSGTLQALQNEIEQRLKPHYPEIWLRID
                     QGSAPSVSVTGARNDKDKERILSLLEEIWQDDSWLPAA"
     gene            74774..75211
                     /gene="umuD"
                     /locus_tag="KLK57_000084"
     CDS             74774..75211
                     /gene="umuD"
                     /locus_tag="KLK57_000084"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBE0890577.1"
                     /translation="MSTVYHRPAAPSGDDSYVRPLFADRCQAGFPSPATDYAEQELDL
                     NSYCISRPAATFFLRASGESMNQAGVQNGDLLVVDRAEKPQHGDIVIAEIDGEFTVKR
                     LLLRPRPALEPVSDSPEFRTLYPENICIFGVVTHVIHRTRELR"
     gene            75211..76482
                     /locus_tag="KLK57_000085"
     CDS             75211..76482
                     /locus_tag="KLK57_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001744069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HBE0890578.1"
                     /translation="MFALADINSFYASCEKVFRPDLRNEPVIVLSNNDGCVIARSPEA
                     KALGIRMGQPWFQVRQMRLEKKIHVFSSNYALYHSMSQRVMAVLESLSPAVEPYSIDE
                     MFIDLRGINHCISPEIFGHQLREQVKSWTGLTMGVGIAPTKTLAKSAQWATKQWPQFS
                     GVVALTAENRNRILKLLGLQPVGEVWGVGRRLTEKLNALGINTALQLAQANTAFIRKN
                     FSVILERTVRELNGESCISLEEAPPAKQQIVCSRSFGERITDKDAMHQAVVQYAERAA
                     EKLRGERQYCRQVTTFVRTSPFAVKEPCYSNAAVEKLSLPTQDSRDIIAAACRALNHV
                     WREGYRYMKAGVMLADFTPSGIAQPGLFDEIQPRKNSEKLMKTLDELNQSGKGKVWFA
                     GRGTAPEWQMKREMLSPAYTTRWTDLPVAQL"
     gene            76686..77312
                     /locus_tag="KLK57_000086"
     CDS             76686..77312
                     /locus_tag="KLK57_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family plasmid-partitioning AAA ATPase"
                     /protein_id="HBE0890579.1"
                     /translation="MIIVLGSQKGGVGKSTLAVSIAAYLMSLGNRVIIVDADDQKSVL
                     TWYNNRPEDLPHIPVTGATGNIKAMLKEHEKSYDFVIADCAGRDSAEMRSGLMAADVF
                     ISPLRPSQMDLDVVPHTCSVFTAAKDFNEDVRGYLVLNMTPTNMFVNEANEAAEVLKD
                     YPEMHLANSRVCDRKAHRDAWAESMTIFETQNDKAQQEIEALVKEVIL"
     gene            77309..77611
                     /locus_tag="KLK57_000087"
     CDS             77309..77611
                     /locus_tag="KLK57_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890580.1"
                     /translation="MRNRANTSPRDEAAFINGGTAGLDNTKKSSDKSSVVSKAKPVSI
                     SFFEENLKDIDALITAEMVSGKGRVNRSDVVRAAVEALKTLSKTEVSRLIAEVKQR"
     gene            78129..78401
                     /locus_tag="KLK57_000088"
     CDS             78129..78401
                     /locus_tag="KLK57_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890581.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            78382..79590
                     /locus_tag="KLK57_000089"
     CDS             78382..79590
                     /locus_tag="KLK57_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBE0890582.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGQEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGCLNKHGQWKKLSFLKDAMRSRWMWNMRQRLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQV
                     YRALGMDKPEPVAKVCYVQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVEGLKKNAR
                     DISLLRYMPA"
     gene            80037..80630
                     /locus_tag="KLK57_000090"
     CDS             80037..80630
                     /locus_tag="KLK57_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proQ/FINO family protein"
                     /protein_id="HBE0890583.1"
                     /translation="MEKNQEERKTPVIVVKKRRTFSLPSLSEKTDIIAPVFTEQTAES
                     APAGINSSAVETHIPEAPARKKKKKRHRFPRPSHWTREYTHECVEKIKALFPHLRAEG
                     GGFIPLKIGINNDISAFLAESPDTELSMDEWFCAVSCITSRRVYLQRTSVAGVPRYGL
                     DGHPDGQVSETEAQSAVQRLAIIEQRWLRMKERQTEQ"
     gene            complement(80912..81499)
                     /locus_tag="KLK57_000091"
     CDS             complement(80912..81499)
                     /locus_tag="KLK57_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine acetyltransferase"
                     /protein_id="HBE0890584.1"
                     /translation="MLIKNPISYFHIGTFLFSGKKRHLLEYWKAEVIMRDKFVFHRLI
                     RNGMQRQRGFLLWWRLANEMYILGNKKQRKCAIKIKNTLMAKYGCDIGLGAQIGKGLV
                     LPHHSGVVIHGNVKIGENVIIRQNTTIGEKESDSRENYIVIGDNVDIGAHTCIIGLNV
                     KIGSNVKIGAMSFIMEEVPDNCTYVTRKESRVIMR"
BASE COUNT        20033 a        22812 c        20738 g        17974 t
ORIGIN      
        1 ctcatcgact ccctgctgaa agaggtgggt ttatccggtg aaggacttac agccctgaca
       61 cgtccactgg tcatgcctgc cccggaaaag gttccggatg tcagcgaacc ggtaaccgct
      121 ccggataact cagaaccaca acaggtttaa tccccgtgcc ggaatttttc cggcatcttt
      181 ttcacaaaga atgccctcac gggatattct ggtttaaaag aggattgaaa atgtccgcag
      241 aaaaattcag agttgtcata tacaccaata catgtgaaaa gaaagtcagt tatattcctg
      301 caacagaaga tataaacatc gctataatgc catttcagac ggcagacaat ggcttaatta
      361 ttgtgtgtca gaactcccgt ataatacgac gcaaagagcg tattcccgga agcagctgtg
      421 aatggaaaga tgtgacatta gcaccaaaat ccagaaaccg cgttcagtta tagcctgaca
      481 gcctgctttt catcccccat aaaagggggt aaaaattaaa tatttcacaa gcgcgatatt
      541 tttcaggaat gcagattatt ttctgttatg accaattgag aaacaggcat gagaacaatc
      601 acaacacgtg aacaattatt aattaatggg agagtccacg aaagaatcgc cacacatata
      661 gttacaggag ctcatgggta tgaaacgctt tgcacaaatg gctataacgt cagatacaac
      721 aaggcgcagc aactggttga aagctgcgaa aaaatagctg aaggtaaact acctgttacc
      781 tgtcctgtct gtttcaccat atggcaggat gtacacagat tcacccacgt tgactttgac
      841 acacaatcgg ggaaaagtga ttttattgat acctgccatt cggagatcat aaccggacat
      901 gtttcaataa caggcaagaa ataaaataat gttccagttc tgaaaaacaa tatcacttgt
      961 tttctggaaa ctatcatttg cagcaggacg tttttctttt tatatttccg gaaccggaat
     1021 gttttagttt tcataatcac cccgaacatg aatgttcatc atcaatatcc tgatgaaatt
     1081 atatttatct gaagacctgt taccagccac ctgtcttata ccgtaacccg atacataccg
     1141 gatttattct atgaaaaaga aatcacttac actccttctg ccgtcattac tcagcggctg
     1201 tacggcattt aatgccacgc ctgttcctga caccacgcct ccggctctgg tgttcgtcga
     1261 cggacaaatt cgcgaaagcg ctgccattat cgcccatacg caaaaacgtc tgcttccgcc
     1321 accggtgacc cgccctgtga cgccgccccg cccgacaaca acagcgacgc ccacaaccgc
     1381 agtcagcagc cagagtaccg cactgcctcc ccgtccgctc cttcaggcca cacagctgcc
     1441 cgcactggtt atgaccggta aacccggtca gtcatccgtc gtttatttac gtgaggcaca
     1501 tcagcgctcc gtcagtgact ggatacgcca gttgctacca cccggctggc agttccggca
     1561 acagaacgct gccataccgg cactgaacaa acgcctcccc gtctggtccg ttaatgacca
     1621 gtggcaccgg gcactgaatg cgctgctggc cggccagcag ctttacggac atatggactg
     1681 gaacaggaaa atactgactg tcacgacctc tgcaacaccg ccggtcgacg tcacggcacc
     1741 gcagggttca cagaaagctg ctgatacacc gcgtaatccg ttcaggggca gcgccgcaac
     1801 gccggcaggc ccgacaccag tgacacctgc cgtcagcgtt tcccgggctc agaccaccac
     1861 cgggacatcc ggaactccag agagcagaac aaccacgcca gccccggtgg ccacgaaacc
     1921 ggtagtgcca ccacagcaga aggtcatacc gccacggcag tggaaaatgg aaaaggggac
     1981 caccctgaaa gacggactgg cccgctggac atcatccgaa aaatgcccgc acggcacatg
     2041 gacactgcgc tgggaaacac ctgtcaacta cagcatcgat gcccctctgg ttttcaccgg
     2101 cagtttccgc caggcgctgg agcagctctt tgcactctat accccggctg aaaaaccgct
     2161 gtatgcccgg accagcactg cacaatgcct gctccgtgtg acagaccgcc cgcaggaata
     2221 acctatggga tgggttacgg gtttcattat ggtcattatg gccattggta atttctggtt
     2281 ccacagtcac ctcagccgta ctgtgcacca tcagcagacc gctgaaatca cacagcaggc
     2341 tgcagacttt attcgttata tgaatgccat caacgattac ctgtatcagc accctgaacg
     2401 acgtgctgcc ggtggtcagc tcacgtcagc acagctcggt ctgcccgcca cgaaaaacgt
     2461 cagccacctc atcagtcagc agcgcgtttt tgtctgggca aaagaaaaac ccggccttat
     2521 gggtgccctc ctggaacaaa gtggtgattc ggccctgctt gccagagtgg aaaacggtcg
     2581 cctcctggac acccgtgggc ggcgtatttc catcacgctc cctgctgtga tccccgatca
     2641 ggtcatcatc tggatgaatt aggccggact ctgaataact cacggattta tctatgaaca
     2701 aaaaacttct tgctattatg attgcagcca ctctgctgca gggctgtacc agtttccgac
     2761 aggcagagaa caccagaaaa gacgccgttg ctgaacaaca acgggcccgg gaaatgatcg
     2821 ctgagctgcg aaaccggaca ccggttgtaa aggaatcccc acgtcagtgg atcaatcccc
     2881 gtccggtcac ggataaaaac cccggtaatg cggtgcccgg ctgtccgatt atcattaaca
     2941 cccggcagag catcaccctt cagcaggtgg cccagcggat ctcgcaaaac tgtcatattc
     3001 ctgtaaggat cacccctgat gtacaggcgt atctgagcgt gaccacctcc ggcagtacgc
     3061 agcaaatcca gggcacagta ccaccgccgg ataccggcgg tatggttcca ctggccgcca
     3121 taggcagttc tgacacgaca cttccccgcc tgcagggaaa tacggcgtca cttcctgaac
     3181 tgaaggcttc aggactgaca tcactgctga atgcggtcgc ttcccgtctc gggatttcct
     3241 ggcgttatga tggttcacat atcacgttct attatcttga tacccggacc ttcccggtga
     3301 cctatatgga cagccaggtt gcctataatg ccaccgtggt cagcggtacc atgtcatcaa
     3361 atggctcatc aggtgggaca gcatcggatg ccatttccgg tgatgccagt aatacccaga
     3421 ccaccaccgt ggatatgaaa tctgcgctgt atcaggatgt gaaaaatgct gtctcatcaa
     3481 tgctgacgcc gggtattggt cgcatgttca tgtcgacggg ctttatcacc gtcacggaca
     3541 ccccacaggt actggaaacg gtccggacgt tcattgaaaa acgcaatgaa gaaatgaagc
     3601 gccaggtggt gctgaatgtg gaaattctga gtatccggaa aacccgtaat gagcaggcag
     3661 gtatcgactg gaacgcagtc ttcagcgaca gaacgctggg actgagcctc ggcagcacat
     3721 tcaccagtgc agccagcgat accgtcactg gtggtgtttc cattgtgaac gggaaactca
     3781 ccggttcaaa agccttcctg aaagcactct cttcccaggg agatgtcagt gtggtaaccc
     3841 ggaattccgc cgtgaccaaa aacctgaccc cggtaccgat gcagattgcc aatcagcaga
     3901 gctacattga gagtgtgacg acggatacca ccgccaatgt cggctcatcc acctccctga
     3961 atgcggccac catcaccacc ggttttaata tgacccttct gccgttcatt ctcccggact
     4021 cacagacact gcagctgctc tattccatga gtctctccga caaaccggtt attgagaatt
     4081 acgagagtgg tggaagcaaa gcccagctgc cgaatgtgga tctgaaaacc atcaaccaga
     4141 cagtggacct gaaatccggg cagacagtca ttatcagtgg cttccagcag tcagggcgtc
     4201 gcagtggaaa acagggtgtc ggcacaccgg gattcttcgg tcttggcggt gggatcaaca
     4261 gtgaaaacga cgacaccatt ctcgtggtgc tgatcacgcc caatatcatc tgatttgcag
     4321 aaggaggact atcgtgacag atatttcacc gacagagcga caggtgtttc cgctgacggc
     4381 aggacggaat gtatttcttg ccggaatgga ctggaaaaca ctgcccgcaa gccataaaaa
     4441 cccccggacg tttgcccgca gtctgggggc agtacgctat atttcctgtg agtatctcag
     4501 tacggaagat accgatcgtc acatcatggt tgcagccgtc agccagaata caatccccaa
     4561 aggcagccgc aggtatttct ctctcgccat gcttatcctg cctctgctcg agccaggagg
     4621 atacgctatc gttgaactgt cacaggcaaa cgatacagaa ctttatggtt ttgtttctgc
     4681 tgttgacggc atcctggtca gtgacctggt gggaacccgg gaggaaatca gggaggcccg
     4741 ggaaatattc ctcaccatta acagcgcgcc tgaacacggc tggacatgtt atgaaccgcc
     4801 ctcgtttaac gggcctgacg gcagaggtcc cctgccactg gaaaccctga caggtgcagg
     4861 gaaatatccg gcagaagcac gccttcaccc cgtatcccgt gggccacagc ttattccggt
     4921 attactcata ctcacccttc tgggtactgc atggtatggc tggcagtact atgaacgcct
     4981 gaaggcagaa aaagcagcac ttgctgaagc ggcacaaaaa gccgcagagg aacggatcac
     5041 accaccgtgg ctgagtatgc cggaaaccgg agattttatc cggggatgca gtgacgtatg
     5101 gtcacgcctg ccggtgtcgg tcgccggctg gcgatattca ctcgccgagt gtgctgccac
     5161 cgggaacagc ggcaatctgc gtgcatcata tacaaacatc gccgggacga cagtgaccgc
     5221 attcagccag cgggtcaggg aaattttcag catccggccg tttatggtta tgccggacgg
     5281 gaatagcggt ggttttgccc tgccggtcac cttcagtatg cctgaaactc ccgttgccgt
     5341 tgaggcatta ccggaaaaca ccctcctgca ggaacggctg accacactcg cccggtcgat
     5401 gcagctgaaa atggactggc aggaagtcag caacagcttc accgatgaag acggaaatac
     5461 catccagccc ccctggaagg agtatgacct gcagatactg acgacacttc cggcgcacca
     5521 ggtggcagag catttcagtg aaccctccgt ccgttttatc tccgtaacaa ggcaactgga
     5581 agagggtcgt ttccgttatc aattcacagg taaatattat gtccagtaaa acaaaagctc
     5641 ttctgctttc cgtcctgatc ctgccgacaa cggtcgcggc cagcaccatc agcaacatgc
     5701 aacagaataa ccggcaactc caggtggaac agattcagga agaaactgca ctgtatgaag
     5761 ccatgctggc aagagaaaag gtgcgacatg aactcagaac cctggagcag ggagaaccag
     5821 ctgagcctgt gcattcacag gaaagcagtt cagccgtcac gacagatacc cctgccccca
     5881 ttaccggtat tccccggctg acagagatat ccggagttca cccccagata accgccagga
     5941 tacaactgcc gggcggacaa actgctgatg taaaagaagg gcaacacatt cccggcacca
     6001 cattccgcgt ggaaaagatc accactgaca gtgttgtcct tgaacaaaat ggtatcagaa
     6061 caacactaca accctgactc agtgccgcca tttacagccg gctgtcggaa tagattattc
     6121 ccccggccct taatcacaaa aaaaatcgtt tccgggtacc gctgcccgta aggagatttt
     6181 atgctggcac ctgcagacca gccacctgat gacattctgg attacgtggt tgttgataca
     6241 gataaaacag gacaaattca tgtcagagtg gtggaaggaa aaaaacacct tcgtgccgtc
     6301 caggattatc tgacccgcct gtgctcccgc tatccgggta aagtatcccc gtttgagttc
     6361 accacacttg atgtgatcgc ccgactcagg caggagacgg tcatttccgg ggtggaaaac
     6421 ggaattaacc ccgtacagca aaaaatgctc gggtatatca gccatgcggc caccctcagc
     6481 gcctcagatc ttcacatcac gccggggcgt gacagtacgg atttcacgta tctggaggcc
     6541 cgtgtccacg gagagctgga gctgctcgat atcgtcagaa aagatgaggg gctggaatta
     6601 ctgggggcca cctattccgg aatgactgac gtcataaagg gaacacagtt tgatcccggt
     6661 gtcccccagg atgcccgtct ggctgaaaga ttcctgaaac tggccggact gtttggtgcg
     6721 cgctactccc actatccgtg tgtgggcgga ctctatgccg tactgcgtct gatcaaggat
     6781 gacagtcagc atataccgac attcagtatg ctggggtatc accctgagca ggagcgcgct
     6841 gtccgaagga tgctgcagcg accggagggc atcgtgatcc tctctggccc caccggttcc
     6901 ggaaaatcca ccacgctgag gacggcttct gccgcctatc tggaacagta tggcttcaac
     6961 gataccggag ggatcctgct cccccggcga cgtctgttca caatagaatc accaccagag
     7021 gggcgtatcc ccggtgcaat tcagacggcc gttatggata ccgctcaggg ctgggtcgat
     7081 tctgttaagt ccgccctgcg ccttgatcct gacgctatcc tgaacgggga gatacgtgac
     7141 catgcctccg ccatcacggc gataaaagcc gccatgaccg gtcacctgat gctgaccacc
     7201 cttcatgcta acgacccgat aaatattctt gagcgtctgg aaatggaggg cgttcaggca
     7261 aggatgattg cagatccgca gttatttatc ggcctgctca gccagcgtct ggttcaggtg
     7321 atatgtcccc actgccgcct cccctggcat gaggtggaga gctcccggac ggatgaagaa
     7381 cgacgcctgg tggaaaactt ctgccagccc gatgccgtgt atcttcgtaa ccataacggc
     7441 tgcccacact gctggcgtgg agtgaacggc cggaccgtca ttgcagaagt gatttccccg
     7501 gatgcgaagt ttttccagat ttaccgggaa aaaggccgga ttgaggcaaa aacctactgg
     7561 caccgggagc tcggcggtat gacacgaaat cagcacctgc tgggcaaaat caacagtggt
     7621 caggtcgatc cgctggctgc gcattacata tcccctgtag acgaggacag ctatacgctg
     7681 ctgcactgat atgaaaattc tggaaagact caacaatacg gaactggaac tgtccggcct
     7741 taaccgctgg ctgggcaaaa agacgttcag gcggactacg ttttacgagt cccttgcagg
     7801 catgatccgg gacggcacac cggttatgcg ggcactggag ttcatctgcg atgtggaaac
     7861 ggattttggt aaaaagaaag ggcagtccgg cctgtatttt ctggcgacag actgtatcgc
     7921 cagcatacgc agcagtggtc agctctctcc ggccctgaag gactgggtgc ccaaagatga
     7981 aattgccctg atccgtaacg gtgaagaacg aggtgatatc gctgaagcca tgtttcaggt
     8041 cgtcaaaacg gcaaaaggac gacaggagat gattagcagt ctggtgagtg tctgcctcta
     8101 tccgctcatc ctgctgacac tctgtgtggt gaatatgtat aacgttcata cccgggttat
     8161 tccgatgatc agcgcctttg caccggaaga acgctggtca gtacagatgg tcattctggc
     8221 caaatcctcc ggactggtta tcggttatgg tttgtggctg ctggcaggac tgctgctgct
     8281 ggcaattctc atcaggatgt cattcagtct gtataccggt ccccgacgac ggtatcttga
     8341 tcgcatcccg ccatggtccc tgtatcagac tttccatggc gtgaattacc tttttaatat
     8401 ttcatccatg ctgcagataa atattccttt atctcatgcc cttaccagaa tagaaaaaca
     8461 tgcagaacat aaccgatggt taaaatcacg aataagtgct atcagaaaaa atgtcttatc
     8521 cggtcagtca ctggcactgg ccatgaaaaa ctgtggttat gactttccgt caaaatcctg
     8581 tgtgaataat cttcttttaa attcagaaag ggaggacagt gttgccagta tggcgcttta
     8641 tgcagaccgc tggctggaag aagcaaaaaa gaatgtcaga aaaagcggca ttattattac
     8701 tgctatatca ggtctgctgg tatttctctt catcatcaat atggtcagtg ccatttacgg
     8761 catcagtgac atgctgaagc agtaaacaca gcatccttta tattcccctc gatccaaatt
     8821 ctgttttacc aatctcagga gctaatatga gcgtcataac ctcttcgtat tcagaacata
     8881 atattcgtca ccttcagaac gatgataaag gcatgaccct gctggaagtt ctcggtgttc
     8941 tggtcgtcgc tgccattgtt attggtgccg tgatgggact gatgtccgat accctgtcct
     9001 cttcagacaa ccagaaggaa ctcaaaaacc tgcagacaat cgccaccaaa atgaaagccc
     9061 aaaaattcca ggggcagtat acaggtactg actacgtcaa aatcctgacc gaaagtggtg
     9121 gcctgcctgc cgatatgatc gccggtggta ataaggcgaa aaatgcctgg ggtggtgcgg
     9181 taatcatcaa agtcagttca gataaatact cctacgttat tgaatccagc aatgtgccga
     9241 agaaaaactg tattgatctg gtcaccagcc tgcgcagttc atccatgttt acaaaaatca
     9301 acggcaatgt gacgaataaa gttgacccgt ctacagtatg taacgcagat aaaactacta
     9361 tcaaactgga aaccaacagt taatcattca tcagcgcccg cccgggcgct ttatttaagg
     9421 tcactgtatg aacaggttat ttatcctgac agtgattttt tcactgttat gctcacttcc
     9481 ttccgcacag gcatccccac aggacaaaca atatgatcgt tgttttatta ccgccggaaa
     9541 acgttattcc attgatccag gattactcaa aattattgcc cgcagggaaa gcagtctgaa
     9601 tccccgggcc attaatcata acaggaatga cagaggaaaa attatcagta ccgattacgg
     9661 gataatgcag attaactcca cacatattcc cgggctgaaa aaaatgggca ttatccggag
     9721 tgagaaagat ctgttgaata atgtctgcct gaatattcag accggagcat ggatcctcgc
     9781 ccggcatttt cagcgatgcg gcgtaaactg ggaatgcctc ggcagttata acgccggctt
     9841 cagtaaaagt aacactcacc gccggatgaa atacgcccgg cagatttaca gcgcttacat
     9901 gcagggacgt tagtccggga gaactgccat gctccagata atcccggcac tgctggtcgc
     9961 cataaccgtg gtaatacagt gtctggccgt tcagatgagt cgctatcgac tctcccggat
    10021 gaatgcccgc ccccctcacc atgggctggc gctgatgagc gggatgcttg cagctgccgc
    10081 cattatcctg cgtctttatc agaatgacag cgctgtcggg gtgctgttta tgacgataat
    10141 gaccggatgt atgacactga tgaccctgac ggatgctgca tcctgccgtc ttccccggcc
    10201 cttcaccatc atgtatctga taagcggcag cgcatttcgt ctctggcagg gagactggct
    10261 gacaggcctg tgcagtgcag ggttctggtt cctgctgttg cttctgttca ggcaatacac
    10321 ctcttaccgc aggaaaacag aaaccatagg cctgggcgat gtttttctga ttgccggtat
    10381 tgccatctgg tccccgccgg gagacatccc ctggattgtc gcaacggcag caggcggagc
    10441 cttactttat ctgtggtgta acagacaacg acgcattacc agagaacttc cctttgggcc
    10501 gtttctctgc gcgtcacagt acgcatttac tttttatccc ggtggccttt ggtaataccg
    10561 gagggaacga ctatgcaaaa agacaacgat aaaggttttg cacttcttga aattctggga
    10621 ggactggttg tcatcagcct gctcatgccg ctgttctggt cttacattga agactacctg
    10681 aatgaaatgc gcaaccagag cgccgcattt catgcagatg cctacaatac tgcagccagg
    10741 acctacatcg ccgataacaa tgcccgcctg catagcggaa cgcttcctgc caccttcacg
    10801 gcggatgagt taatccggaa aggatatctg aagggtctta accgctcgcc ctttgggcag
    10861 agttatacca ccggcatccg tcgtaacacg agtaccggtc gtctggaggc cctgacctgc
    10921 tccaccggag gggagaatat caaagacgat gccctgcgca gtatcgccag cctgctcccc
    10981 ggtctgggcg gatttatagg aaaaaatggc acagcaaccg gtgtttttgg tggatggaca
    11041 gataaacccg gcgactatgg actcagctgc aatggcgggc acatcgccat cgtaatgatg
    11101 ggtgatgacc tgcaggaaag cgatcgcctg tatcgctttc aggtaccggg acgaccggag
    11161 ctgaaccaga tgaacacggc catcaatatg ggagggaaca acctgaacaa tgcaggaaac
    11221 gtcaatggac aaagtgcaac actgaaaggt gatgttacaa gcgaaaacgg ctggttaatc
    11281 accaaaaatg ataaaggctg gaagaacatc acctatggtg gcggttttac gatgacagac
    11341 agccagtgga tacgcgcagt cggtggtaaa ggaattataa ccacagggga aatcaaagga
    11401 ggaaaagtca gtggggggac agtccggtct gacggtcgcc tttccacagg tgagtattta
    11461 cagctggata aaacagccgt agcaaataca aagtgttcac cagatggact tgtaggccgc
    11521 gacagcaaag gggcaatact ttcgtgccaa tccggtgtgt gggttggctt tgaatcctac
    11581 cctgtgggaa gccctatccc gtggccatct gccactcctc cacaaggcta tctgttgatg
    11641 aacggccagt cgttttcctg ttcccggtat cctcagcttg cccgtgctta tcctagctgt
    11701 aagttacccg atctgcgcgg tgtttttatc cgtggctggg ataacgggaa gggacttgat
    11761 ggtgatcgga acagacagct cctgagttat caggccgatc aatcaggcgt ctatcacgaa
    11821 cgtggcggat ggctgaaagg gcatcatagt ggtatgccat actgggcaca ggggagtacc
    11881 acagaaatgc gtcccaaaaa cattgcattc aactacattg ttaaagccag ctagggactc
    11941 acatctgata ggtatacaca gagaaaacac cgttaccaca ggcgtaattt tgtgttggtc
    12001 tggagatgat ttgatatgac gctcccactg gcacggcaaa tgaaataaat ctccgggcat
    12061 atcagctggt cagcaaactc gatgccaggc gtaaacagac cagcaaaatt tccccttatt
    12121 ttgttcccta ccctgcaact gtcgggtgca gaaacgggct gtttgtcgtg acactgcacg
    12181 atttcgacct tgaaacccgt gcagaaaccc ggggactggc aatatctcat gccagcatgc
    12241 tcagtcacct cctgtccgaa tccttttcat tcgcccttaa aacagagttg caaacactct
    12301 tgaaacacac tcgatcataa cgatcgaaat aactcgcttc cgtttaaaaa acactctctt
    12361 tagactcaat ttatgaacaa aacacgctga cccgcatgga ggtgaaatga aaactgacag
    12421 cacaggtatt gccgcccgta tgatgctctc cctggacagg gagcgaattt gcgaatgcct
    12481 gctcagtcat cgccagctgc agtccacacc cctgcaggtt cgttatccgc agggagtcag
    12541 ggatgccctt ggtatcatga gcgaacagct ttctctttcc gtctctgacc tgacgcgtat
    12601 cctggtcgaa gacgcgttaa gtgaaatgtt tcttcctgca gataacatcg tccgccgtct
    12661 tctcagcagg atggagcaca tcatgcaggc acatgatatc agcgcaacaa cgatggctgc
    12721 attactggcc ccctggaata tccgccctgc cgtcttccgg gagccggaca gactcactga
    12781 ctatctgacc ggggagattc ttgcagcact ggctgactgg ttttatctca gtccggaatg
    12841 gctgaacggc agggtgcatt atccactgta tcgtccggga gactggccag ccacacagga
    12901 gatattctgc aggattattt cagcccgcga gaatatggac attattctct ggcatggttt
    12961 tccgtttgcc ggaacgcatt ccggagaata ttgcggcgtt ctgctcaggc agaaaaaaga
    13021 aattaataac accattattt atccggtact ttcgttatat ccggccagaa tggatataga
    13081 aaaagaaggc tggtttcaga tggcccgaaa aatatctccg gatatccctg ttcgcgccgt
    13141 aacactcact ccggcacagg cagaatatct gataacagga aaaatattac ccactgcgtt
    13201 attcagggtt ccgttatccc cctggtaata atactgagca ataaaccgga ataatacacc
    13261 aactgttttt aatggcacag ccgtgccctc tgttgttata cccaaaatac ctcaatacag
    13321 gaaactgaaa tgatgatgaa aaagcatatt acccgtactc tcatcgcgtc tgccgtttta
    13381 ttttccttta attcgactgc agcaacgtca tattttgaag cccgcaatga tgcgatggga
    13441 ggaacggggg ttgcctcctc tcactatggt gtggccccac tggcaaaccc ggcactgctg
    13501 acaaaatccg gtgcaaaaga tgacttcagt cttctgcttc cgtccgtggg tgcgcagctg
    13561 tccgatccgg gtaatatcgc cgacaacgcc gacaaaatca gcgatgactg gaaagccttc
    13621 gaccgtgccg tcgacagcca ctccggggta ccgcagacag cggcccgcct gaaagagaga
    13681 ctgcaggact tccggaacac ccatgccagc gcacaggccg gggtatctgc tgtggcggcc
    13741 cttcccggtg ataccctcgc tgctgcactg atgctgaaaa cgctcgggac tgtcagcgtc
    13801 gacggtaaag tcagcgatgc cgacctgaac tatctggaaa gcattgcaga cagtggcagc
    13861 caggatgtgg ataaaaacag actgacctca caggcttttg cccgtgccgc actgatcacc
    13921 gatgtgggcg ttgcgctggc cactgaactg gaaacggccg gacaaaaatg gtcactgggc
    13981 ttcacaccaa aattccagcg tgttgacctg tttaactaca atacgctgat caaaaactat
    14041 gacagcagcg cattcaaagg tgaccgttac cacaacaccc aaaacggtat taacgctgat
    14101 atcggtgcca gcatggacct ggatgacaac tggacgctgg gtctggtcgc acagaacctg
    14161 atcccacgca gcattgagac caaagaggta catggtgtca ctgaaacctt caggatccgt
    14221 ccgcaagtaa caaccggcgt atcatggcac aacgacatgt tcaccacggc actggatgtg
    14281 gatctgaccc cggccagcgg cttcacatca gacagcaaac gtcagtttgc cgccgtaggt
    14341 gctgagttta atgcctggaa atgggcacag ttacgtaccg gttaccgcca gaatctggcc
    14401 agcaacaacg gcagtgcatt cactgccggg attgggctct caccgtttga tgtgattcac
    14461 attgatgtcg ccggcctcgt gggcaccgac aacaactacg gtgccattgc tcagttacag
    14521 ttcaccttct gattatcacg ccccgggacg cccggggctt ctgacctgaa tctgctcgtt
    14581 tccgctcttc tggccactca ccgccagtcc tgttcattat tttccggata aaaccatgca
    14641 aaaaaaacgt atcacccttg cagggggagt tgccatgctc tgtctgagcg gctgtcatct
    14701 tcccccggat aataccgcac cggccaggaa gtacggtgca gcttctgaag tcgctgtcac
    14761 acgacatgca cagtatcagt tgtgggagca gggcacgtat tctgcagccc tgccttcccg
    14821 ccagcccgga ggcagcctgc aggctcacag cgggcgggtt tcctttgact gggaaggcga
    14881 tgctctggag ctcctgagcg agctggcccg tctgcgcgga caacagttca gttacagcgg
    14941 cgtgcgtctt cccctgcccg tctcactcca tgtacgcgat atgaccttcg ctgatgccct
    15001 tcgtctcaca gaggcccaga ctgcctggcg ggcagtcctc tcacagtcag ccgggcgttt
    15061 acatctttcc tatatgccaa cggagaaaaa atgaaaaaac aggtctgtct cctgctgtcc
    15121 gggctcctgc tggcaggctg tgccggacag caccatcgcc cccctgccgg tatggcaggc
    15181 aatttaccac cgccggatct ccgggcatgg ctgacgccca ctgacaacca ggatgacggc
    15241 atttcagaca cccgtcgcca gatgctcacg gacgccggac gaacgctggg attccgtggc
    15301 ggaaaagcac agcgctcatg ggaactgatt caggcactga atgcccggga aagtacactg
    15361 aatgccctgt atgacttccg tccccttatc agcagggaag gctggctacc ccctgttatt
    15421 gatgaagcgc aggatgttgc tcacattcag cctgaccaga taagaaccgc gtcccgtgtc
    15481 tggaccatcc tgcgccccga acgttttgtc agtaacccgc cgggctggcg tgactggctt
    15541 ctgcgggggc tttccactac cgccaccccg ggaactgagg gccgtgttgt accggaagac
    15601 cgcgcacagc gcagactctg ggaaaacgca ctccgccagg gctggcagga aggccgtgac
    15661 aacgctgacc tcaccctgga agccaaccag aaacgcctga cccgggatta ccggggaatg
    15721 atgctttatg ccctgctctg gcgtcagggc atgatcacac gcccggatgt gactgaacag
    15781 cggcagaccg tcaccggtaa cggcaggaaa ctcatcaccg gagaccacgt ccgccgtctg
    15841 aagacacacg cggaattcac cctgcagaaa tcacgctggc gtccggtcgt cagcacggaa
    15901 ggagcccccg atgaaattac ccgatgagtt cggcacattc cctttcgccc gtttcacggc
    15961 cgaagagttt cgctattttt ttgcctggtg tgcccgacac cgcgtcagtg atatcgatct
    16021 gacaggcggc tcaccggtat ctgtcagtcg ctttggacgg cgggtacgtt cgtccgcaca
    16081 tcctctgccg tcctccatca tgatggcgct gagtgatgaa ttgtttggac gggaagtcct
    16141 tccccgggtg ctgtccggtc atcccgtgga ccgtaccatt caggtcagcg gtgatgcctc
    16201 cggtcgctac ggtctgcaac gggggcagcg tgttcgcctg cgctgtcatc tgattcaggg
    16261 aacatcagca acagaagaaa aagtaatctc cgtcaccatg cgggtgatcc cgtccgatat
    16321 tccggacatc ctcaccatga atattgaacc ggacctgctg gatgccatgc tccggaaaac
    16381 cggtctgggt ttcatctgcg gagagaccgg ctccggtaaa tccacactgg cagcggcact
    16441 gtaccgctac attcagacgc atttccccga ccgcaaaacg gtgacctatg aagatccggt
    16501 ggaatacatt ctggggggag agaatgacct tctgccaccc catcaggccg aaatcgggcg
    16561 tgatgtggaa agttttgcgg caggacttcg ctctgccgtt cgccgtaacc cggacatcat
    16621 cggtatcggt gaaatcaggg attatgaaac ggcagatgca gccgtccagg caggtaacac
    16681 cggtcacttc tgcatcggca ccatgcacac caaatcaccc ggtgaaacct ttgcccggct
    16741 gctggggtta tttccaccgg aaatcaggga ttcaatggca gccgccaccc tttccctggt
    16801 gcagttcatt ctggtgcagg tcctggtcag aacaaatgac ggtggcagac aggccgtccg
    16861 ggaatacatc gtcataactg acgaactgcg tgatacgctc tcccggcagt cccatgcgac
    16921 atgggggcat tacattgatg aaatcattcg caaggaaaaa cggcggatac gggatcaggt
    16981 tctgaccatg tatcagacgg gcagcattga ggcatcagaa gctgcgctct tcatcccggc
    17041 cggagaattc cccaaatgaa acgcgaaggc ttctggcggg atgtcactcc gccactgaat
    17101 atttatggtg tcccgttgcc gtttaccatg atttacctgc tgctgtttcc gtatccgtca
    17161 aaaacaacat tctgggtgtg cagcggcatt ttgctgtttt ttgtgctcct ggggtactca
    17221 ggatggactg tccggacgct ctgccggcgt ctgtacagcc tggtgcaggg taaacaggcg
    17281 tcaggccgtc cctggtggta ccggcatcgc acagaattac cccgtgaatg gcgcggaacg
    17341 gaataaccct cttttgttca tcatcccggc gtatcacaac gccctcatct ttaatccccg
    17401 gagaacgaca tgaagtccag atatcgaacc tggcttgctg tcccgcctga ggagacagaa
    17461 gccgtaaaaa atgcagttcc cccgttaaat ggccgcaaag ctgtcgcatg ggatccagaa
    17521 aaaaagctct ggtatgcccg ggcgggtacc gaactttcac tgctggagcg ctggttaccc
    17581 cggccacagg aactgtcgat ggatgccggc gatccggtca ctgaatttgc tcaggtcctg
    17641 gaaaacgccg ggctggttat tcagggattg ccacagatgg acggagccat acacagagtg
    17701 accacccggg atgacaaaaa aggggccaaa agtggcgcat acagggccta tcttgatggt
    17761 cgtcccgccg gctggtaccg cgactaccgg agcgccgatg acagtcccac aaactgggta
    17821 ttttccggcg gagaacaaca cgatcctctg gccagactgc atctgcgggc attcgctcag
    17881 caacagcgcg atgataacgc tcgtaaactg cagcaacagt ataacaaaca ggccggctat
    17941 gcccgctcct atattaacag gttgccacag gcaacagcac atgaatacct gacgcgcaag
    18001 ggcatccggg ccgcgcctgg cgtgcggctg aacaataaaa atgagctggt tattcccttc
    18061 agcaacggcc ggggagaaat acgttcgtac cagcgtattc cggtcaccgg cgggaaggat
    18121 gcccgcattc tgaaagactc cgagaaaacc ggcaactggt tcaccttcgg aaccccggaa
    18181 aatggccgcc ccctgttatt tgcggaaggt tacgctactg ccgcctccct gcacgaagca
    18241 accggccttc cggttctgat gacagttgat gccagcaata tgattgccgt ggcagaaaat
    18301 gcccgccaaa tctggactga cagccccttt gtcttctgtg ccgacaacga tcatcaacgg
    18361 gaaattaaca aaggggtttt tagtgcaaca aaggccgcag aagtcacgaa tggcgaagtc
    18421 attattccgg cctttacgga ggcggaaaaa gcacagggac tgaccgattt caatgacctg
    18481 gacgccagcc ggggacggga caactttcag aatgccatga atgcacagtt aaaacatatc
    18541 ggtatcccca cacccaacag tgacactgcc gaccatcgtg aggctgtcgt gatcgacaac
    18601 ctgatattca caccggtaaa agatgagaag ccacaaatgt cccctgaaaa cagacagagc
    18661 acagccccgg aaacggagct ggcgacacag gatacgcctt atgacaccca ctccgacgat
    18721 acagaaaacc aggcatatga agatgactat gccgcctatg catccctgca atcagtaact
    18781 gcggagacgt tatccccgga taaaccactg gagaatattg cgtctgccgc tgataccggc
    18841 acagagaata cctctgtgcc taccgctggc gaccaggaag acctcaatac agacgccaca
    18901 ggtacagaca ccgctcacaa cagcgacaca gcagaaacag gaacgcttcc tgaacctgcc
    18961 gggatgcagt ccgtgcagca ggcaaccact gaaccgaccg cagaagataa accacacgag
    19021 aacgttgttt ccgctgccga taccagagaa gaaaatgcct ctgtggctac cgctgatacc
    19081 ggtgaagaaa atgcctctgc caccaccgct gacaaccaga gcacgcaggc agaagaacat
    19141 ccccggagta aagacacacc ggaaacctcc ctctccgacg cggccgggac ccagcacacc
    19201 accacaacgc agtctgaaga acaagtgcag gtttccatgg ccagcgctga cgactggagc
    19261 gcatttgcgg aagatctcaa cgctgacact ccgggggccc ccactgttca caacaccgac
    19321 aacgcggaga caaacacagt ttctgaaccg gccgggatgc ggccagttca gcaggtgacc
    19381 actgaactga cggcagaaga taaagcacag gaaaagagcg ttgcgtctgc cgctgagacc
    19441 ggcgcagagc actcctctga catcaccgat gacaacagga acgcacccgc tgaggagctc
    19501 aatgccgatg ccacggttgc tgttcacaac accggcacaa cagaaacagc ctcaccttct
    19561 gaacctgcag aaactcagcc agtccggcag gccaccgctg aaccgacggc tgaagataaa
    19621 ctgcaggaag agcgtcttgt ttccgccact gagaccagca cagcgaatga agcagcccgc
    19681 cctgaaacgc catcgctttt cagccgtctg cgccgttttc tgaccggtgt gaaatcacgc
    19741 acctcagaac cttccgttat tcaggagaaa aagcccacgg aggctgaaac agcaacagag
    19801 gaggtcgcac caccatcacc accggaaacc agcccggaag acacatcgtc agcggaaccg
    19861 gacacgatta tctttgcacc ccgccgtccc gacagtccgc agcggcacaa cctggacgag
    19921 atccttaaaa atcttacaca caaagagttt cccgacagga ctgcgctcta cagcgttgac
    19981 ggtgaacccg ccttcctgga cagactctac tgtctcgaaa tggtggatgg cgccagcgcg
    20041 gatgacaaaa aggtcctggc tgcccttgct gtggccacca acttctatgg cggcgttatc
    20101 gagctgaccg gcagtgatgc ctttaagcaa aaagccatgc agctgattgt cgaatacgac
    20161 atcaaggttc gcatgaaact cccggcccag cgggcagcac tggaaaaaat ccgcaaggaa
    20221 aaagagatgg cgcatgatgc cattgtcacc cacagaccca cgccggaact caaccgccag
    20281 tcaccggaag agacaacaat ggcccaaccg gctccccctg tgtcaccgaa ggcagaggct
    20341 gaacaaacag caggcagtac ggctgaaaca ccgcatccgg aagcgccggt cacggcaaaa
    20401 gctgaagccc cccgggccga cagaccggaa caacccgccg ccccgtcgtc tgcgccagaa
    20461 aaagcccctg tcagtgcaca gccgcagcaa cccgacacac cggaggaaga gccgcccggc
    20521 aagctcagcc ccggtgaaag tgtgactgcc gtcctgacaa actacggaga aaaagagtat
    20581 gcgcccggaa aagggcgctg cttttttgtg gaactcacca accgcagtgg caaacgggaa
    20641 tactggggaa acggcctgga agaactggtc aaaaaccatc agaagggtga ccccgtcaca
    20701 ctgacactgc aggcgagaga aacattccgg ctgtccggtg aaacgcggga tcgtatccgt
    20761 aatatctggg ccataacccc ggtcagtaac ggcattaccg ttaataatga ccaacctcat
    20821 gcgggtcagc ataccctgga gtttccgatg gcaacattca gccaggtgat gttacagatg
    20881 caacaacact ggccacagat aatgcaggaa gttcgctccc ccaaaaatcc tcccaataat
    20941 ctgtacattg gtgaagacag gcaacctgca tcagcacctc aggatcaatc aatgcgactc
    21001 cccctcgctg gtaatgcccc caatgaactg ataccagcca tcgcctctgc cgataaggac
    21061 aatcgtcagt tgaatcttct gctggtccac agcgcagatg atcatctgca gggcgtggtc
    21121 cgcctgaacg gcacccttta cccggcactg gccaccccgt cagctgacaa ccgtcagctg
    21181 gttatcaatg ccctgacgga taacggtctg cagtttgccg gttacggtga agctgttaac
    21241 catgatgaga ataatcatca gcgtccgtcc ccacaaataa tgcagtttca cctgaagcaa
    21301 caggactccc cgttgtttgc cgccatccac aaaccggaag agcaaccgga caagcttttc
    21361 cgctctctgg gttttgagca gacctggaaa gagtggtctg acagccaaaa ggcggaggac
    21421 aggcaggaaa aaactctgca acaggcacaa tcccacagcc ccggccgatg agccggtaag
    21481 gacgaccatg aacatgaaaa aaacgctgct ggtcatactg accggcatca ccctggcatc
    21541 accggtggca ggtgccgcca cctgccgggc gggggccgct gccgaagccg gcagcagagc
    21601 cgcctgggag cgggcgcgca cagccagcca ggcatggtca cagcgggaga atgacgtcgc
    21661 ctcctccctg cagtcctgcc tgtcccgcat caaggacatc agtatcactc ttccgcagtt
    21721 ccccagcctg gacaagctcc tccgcgacct ggaaaaccag gtctgtgatg ccgtcgtgga
    21781 tcgggtgaat gaccatcttc cggggaatat cgacccctgg aaagactaca acctctgaac
    21841 aggagcacca tgagcgaagc acaacccacc tcccctgtct caccggaagc ggataacagc
    21901 ggagatattt ataaccccgt gctcagaacc atcgcacagt cagaacaacg ggccgcgctg
    21961 gtacccggtc tggtcagggc gttgctgtac agcggcgcag cactctgcat cagcctggtg
    22021 gctaatggca tacagtactg gcatgccacc ggcgtgacgc gcgaatactt tgccactgac
    22081 aatggccgac tggtccgtct ggcaccaacc agccagccgg cctggagtca gagcgacgtc
    22141 atgaactttg gcagcatgac gctgagcgaa gccttcaatc tcgattttgt ccactaccga
    22201 aaacaaatca cgacacaggc cccccgcttt tccgaagccg gctttgccgg ctatgtgaat
    22261 gcccttcagg cctcgaacat tctcgacact attaaaaagg agaaatttaa cctcacaacc
    22321 actatcgggg caggtaccct ggtcaaccag ggacagatgg agaacggcgt ctggttctgg
    22381 aaattccagt atccggtcag gatgcgcctg gtcggtcaga ccagcacccg ccccgaacag
    22441 gccttcacct ttgaaatcac tgtccggcgc gttgaccccc gcatcaaacc cggcggcatg
    22501 gaaatcagcc agatggtttc ccgtaacgcc gcagcaggaa tctgagactc atatcatgca
    22561 catacatcat cttattggcg cactgcttat tgtgtgcggc actgtcacag ctgcagataa
    22621 cccgggctgg aaaaatgcac gaagcccgca gaccggcgga aagaccgcac aggcaaccgg
    22681 gaacaccccg gcaggcggtc ccccgccagc cgcacaactc ccgggaaacc tgccgcctcc
    22741 cggcgctgct cttgtacagg aaggcgcatc gctggattct cccctgaatg ccagtgaaat
    22801 tcgtgaactc cgtgggataa tggccgataa cgaacgggcc atcagtgccc cgctcaccag
    22861 tgtggtgccc cgtatcagtt ccctcacggt gaatctgtct ccgggggcca gcctgcccct
    22921 tgtccgtacc gctctgcata acgtcagcgt ggtgaccttc acggacgtca acggcagtcc
    22981 gtggccacag tcggatccgc cctataacgc tgcgcccggt cactttgacg tgcagttcaa
    23041 cggcaatatg gtgtccgtca cccccctgcg cgcctgggct gccgggaatg tgacggtcta
    23101 tctcaaagga ttgtctgtac cggtcatcct gaatatcacc agtggtgaaa cggacaccac
    23161 taccctgagt caggaaatcg acagtcgtct ggatctgcgc attccccggc ggggacctgc
    23221 cagcccggtg gtgtccatac cgtcagaaaa aattgccctg catgacgcca ctctgcaggc
    23281 ctttctggac ggtgtgccac cggatgatcc cgccatccgc cgcctgaaat tctccggtaa
    23341 cgtcccggac accaccatct ggcaacacgg cgatgatctt ctcgtgcgct cacgggccat
    23401 gctgcgggat gaatttgaac agacgctggc atcggctgat ggtacgcggc tgtggaaact
    23461 gccggtgacc cccttactca ccttctccgt gaatggtcag tccgttcacg tcaccccgga
    23521 actggagtaa atcatgagcg cagaacaaga acaggacaca gccgcaaaat ccggcaaaaa
    23581 gctgtcagcc ctgctggtta tggggggcgt gattatcatc gggggcggat acctgctgct
    23641 cacctggctg tcccccgaaa acgctgcatc gctctcgtca gtcaatatca acagcacaac
    23701 gtccggcggc ggacggaacg tgatggagac acagcactac cgggatttac tgcgggctga
    23761 caatgaacgt ggtgctgcag aagcgttacg taataacgag acgtttatcg ccagtctgcc
    23821 ccggggactg gataccgata ccggaaacga tcagaaaagg gataatcccc ccgatgatca
    23881 gtcaggcact tccgggcaaa acgcatcccc ccctcaggaa gatcagactg cccgggaaaa
    23941 acggcgtgag gcattacaga aactgcttgc ccgtatcaat acacagcatc aggatgcccg
    24001 gccaccggta gtggcagcag ccatgtggag caccaccggc actcccggga cagccccaca
    24061 tgcgtcaccg tccggtaccg ttacatcaca gcatccccgt ctgcaggaga cgagtctggc
    24121 aacaccggct acacgtcagg ggatccagct gataccggca ctgacccgcg taccggctta
    24181 catcgacacc gaagtggact ctgacaatgc ggtctcccgt gtggtggcct ccgtacctgc
    24241 cggcccatgg gccggggccg tcctctacag ccaggacgcc agactcgcag gtcagggaat
    24301 ggatatccac tttgaccgga tgatgtggcg gggcatgttt ctgaaggtga atgcatatgc
    24361 cctgaatgaa cgaacaggaa tgtcaaccgt ggccagcgac gtgaaccacc gctggtttaa
    24421 gcacatcgtt ctgccgtccg tacttggcgg ggtgggcaat gtcggaagtc tttacaaaga
    24481 cgccaacaca cagatccttc agggaaacta cggcagtatt accggcaggg tgggaatgcc
    24541 tgatggagag gcgctggccg gcgtcattgc aggtggcatg gccgaacggg gcagccagct
    24601 gctgacccgc caggcagaga gcgaacctta ccgtcaggtg acagtatcac ggggcgaggt
    24661 cgtctccatt ctgtttgttg agccggtcat gagcaacgat atcagtacgc cgtcctccgg
    24721 cactgcccct tctgagtccc cctcctccgg acgtccgctc acccaggcgg aggcagagca
    24781 ccaggcctct gacaggatcc gggcggcggt cctccgtaaa cagaatgaaa tgcagcaacg
    24841 ctacggcata caacaggaaa gttatccatg aaaaacgaac atgaagatcc cggcagcttt
    24901 ggcgaaacga cggcaccggc atccggcaaa tccttcctga aacgccagta tatgggatac
    24961 agcctgccgg tctgggccgg ctgtgccctc atcatcctgg tcgccatctg gtacaagttt
    25021 ctgcggacac cctcagagcc ccctgcagat gcaggctttg ctgtcacacc gcccgctgct
    25081 gagagctggg cagagcccat gaccatgacg gagtccggga tcggaaacaa catgcccgca
    25141 ccgccggtta ccccacaggc agacatgcag caacttgccc gtgatctgaa agcagagctg
    25201 gacagacggg atgaggaaat ccggcgcacc ctgaccatgc tgaaggacag tatcaccacc
    25261 ctcagtaacg ccattcagaa ggatgaggcc tgggcacagg aaacccgtca gttgcttgag
    25321 gagacccgta agcaggtgca taccctgcag atgcagggag cactgccagc cccgaccggg
    25381 aataacagac agcctgcgaa aaccctgcct tcacctctgc agggcatgaa aatcatgtcg
    25441 gtggagaaag gcatggcctg gatccgctgg cagggcagtg cctgggcagt acgggaaggc
    25501 gacagtctgg gaaacatcac catcacccgg attgacccgt ccagccgggc agtcatcacc
    25561 tctgccggta cattacgatg atgagcattg atgccctgac agccatagag aactttgcca
    25621 gcggtatttt cagtgcaggc atggagtttc tctttacctg gggagagctg ctggggatta
    25681 tcggccttat cggtcatctg atgcgcgccc gggcagaagg acgtcacagt atgggaccgg
    25741 gaaaatttat tgccggtatc ttcatctgcg gcatgctggt cagtctgccc tcaatgatca
    25801 atgccggtgg tacccagatg ggcttcaggg cagattcttt cgcccccatc gcatacgtgc
    25861 agccgggctc ctttggtgcc gctgccggtg ccgtcaatgc cattctctca ctggcaaaac
    25921 tggccggcgt cggttttgtc atgaacggca tatccatctg gcgcaaatca ctgctggacg
    25981 ggcacaccgc cctgagcgcc agtgaatcca tcagtaaagg gaacgtcaag tttgtggccg
    26041 gcgttctgct ggttttcaac gaccgggtta tcgatgccac tctggcaagc ctcggtatcg
    26101 ccttctgacc cctgtatttt ttcagtaaat caatctgtta ataaaggaga cactatgtca
    26161 cgacttcgtg ccatggcggc gcgtctgcgc gctgcatctg tttatgttca gttatgcctg
    26221 atggcctgcc gtgaccggct ggtacgcctg ttactgctgc ttcccgtgat gctgctgccc
    26281 cacaccgcaa tggcaaatac cctcttcgat atgattaaaa ccgttggcga tggtgccaga
    26341 ggctccagca gttcgtttgt ggctatcggt caggttattg gcctggtgct ctttatcggc
    26401 ggactgcttg gcctcaaaca aatcggtaaa aaacccggtg cagggctggc ctgcctgctg
    26461 tcaattctgg tcggcgtctg ccttgccgtc gtaccggaac tgatgggcag cggacagaaa
    26521 acgctcggtc tttctccggt ttcaatcagt taatccgcaa aggcaccggg aaaccggtgc
    26581 cgtcttcaca caatgaggag gaccagacca tgtcctgtga gctaccccct ttcaccctgc
    26641 tcagaacaga gcacggcaac gtgattgccc gcgacaaccg ggataatcag gagtttgtgt
    26701 ttgcagagtg ttactccacc gctatagcag cactgattct ggctgccgtc accctgtata
    26761 cagagagaaa agcgtatgaa aaagaccccg ccactcatcg ccccggtccg ggaacgatgg
    26821 cttttcccgc tcccgcaacc ggacacggta ttcagcggtg aagccctgtt accaccgcca
    26881 gtgctctcaa cagagggacg ctgcccctgc tgtcgcagga ccgtcaccca ccgcttcatt
    26941 ctggaagatt catggccatt acagcagatg gctgacacct gccgggatac gatcgtcctg
    27001 ctggaaaaaa acctcacccg ggtgatgcgc ctgaaaaaac gtccggtacc ggaaaacgct
    27061 gacgaaaaaa agaaacacac ccgaaccctg caggatgcgg agcggtccct ggcacaggcc
    27121 cgcctgtcgg ccagacgact ggccctgcgt catgtggaaa agtcgcaaat cgtcacaacg
    27181 gatgcactca gcgaaaatga aagtgacctg ctgcagccgg aaggcccgcc ctttcatctc
    27241 tgtgccttct gccacgcctg gcactgtctg aacggctacg ctgccgcaca gggagtgatg
    27301 gtctggctcc ccgatttgca tccggcctcg gtggttgccc tcaatgcgcg ggcactgcag
    27361 gagattttca gcgataaccg tcagcgggtc aggcagggac gggcagtcct gaatgccctg
    27421 gtacagaacc gtctggccgt tgaggagaaa ttccgcacct ggcgtccggc ggattttgca
    27481 gatgccctgc gccgctggcc gccggcacag cgtaaaacgt tgagggagaa aatggacggg
    27541 gtggcgctca ttctgctgcc ggattcgttt cctgacaaaa agtacgtcat gtgacacaca
    27601 ccggtgccgg tgacgttttg ccggatatca gttctccgca gatgcattcc ggcactttat
    27661 tatccggtcg ctgactgacg atattccgcc tgacggagat gaaaaagaga ttgcccggtt
    27721 cattcgccgc cagcagttta tttatccggg tgtaatcaca cagaaatacg cccctgcccc
    27781 tcaacatatt atttatcttc tctgctgttt tttcatgcca catcccctgc tgtggcatca
    27841 aaaaatataa gaccttgatg attcatcctt accggagtgc cccatgccat gaatatcaac
    27901 cgcattattt ttgccattga agactgtatc agtaccctgt cacggtattc cgtttccgca
    27961 ttcttccccg attactgtga tctgcacacc gtcatcggtc tggaccagca ggaccgcgaa
    28021 cgccgcccct ggctgaaagc cccctacatt gccaccacaa aacaggggta ttacctttcc
    28081 gtctttgaaa tcagcggtgc cctgaaagaa atggatgaaa acccggacca gacggccccc
    28141 gggacactgg agtcactgat tgcagcactg gctgagcgaa tgaacacggc ctggaaaaat
    28201 tccggccaca aaatcagttt tgttttcgaa cgcgatccgg agcgtggaag ggatgaaatc
    28261 gaagccatgc tcttacccca gcgaaaatcc atcgcacgca cggggatcct gcttcaggac
    28321 gtcctggatg aaaaagtcac cacactcacc ccctggcttg tccgggagcg ctgctggctg
    28381 accgtctgga gctctgaaga actgctgagc cggaccgatc gcaagaacca tcagacccgt
    28441 gtcaggaaac tcgctgaaca cgctccgcca gcccgttttg cgcaggatcc ctggcgctgg
    28501 acactttccg ccctcaaaat acggcatgac gccttactgg acacgctgga gcaggctctg
    28561 acacatgatt ctgatggcct gctgattcgc ctgatggata tccatgaagt gggacgggaa
    28621 atccgccgcc aactggagcg caacagtaca ccggcagtat ggcagcctca tctgcccgaa
    28681 gatgcccgtc ctgccggctg gcgacagggc ggggatacct ctgtattcca cgcaccgtcg
    28741 ctgaatctgc agttattcac cacacagcct gaaacacacg gcagcctgat tcaggccggt
    28801 gagctctggc acggtatggt cgccattacc ctgccccctc agaatctgcg gacattcaac
    28861 cagctggtca gggatgtgcc ccgtgcaatc ccctggcgta tccggatgga tctgatgccc
    28921 gacggcatga aggcgctggg ggtcaagaaa accctgctga cctacagcag ctttattcct
    28981 cctctccggc ccatgtatga ctcggtaatg atgctgaatg acatcaacaa gcatgatccg
    29041 gtctgtatca tgaccattgt tgccaccaca tggggaaaca gccgggaagt ctgtacccgt
    29101 aaccaggctc tcctgaaatc tgcgctggaa ggctgggggg tatgcggcac caccacaacc
    29161 ttcggtgatc cgagacgtgc ctgggtgaac acggttctgg cagcctccca cagctccggc
    29221 ccgatccccc tttacccgcc cctttcacac gccctttccc tgtttcccct gaacagggca
    29281 ggctctgtct ggcggggaca gggaaatctg atgatgcaca ccgaagacgg ctctgcatgg
    29341 gaggtggcgc tggcgtcatc acaacagaac aagcacacgg agctgacacc cggcgcaccc
    29401 ggtctgggca aatcggtact catcaacgca ctgtcggaga tccagatagc ctcagcccag
    29461 aagaatctcc ctttcattgc ctatatcgac aagggattca gtgcacaggg actggtacag
    29521 cttatccgtg acagcctgcc tgagcagaga aaggacgaag ccgtggggat cattctgagt
    29581 aatgacccgg accacacccg caacctgttt gatgtgatgt acggggccag aaaacccgtc
    29641 acaccggaaa agaacttcat ggtctccgtc ctctgtgcat tgtgtgtgga taccggcacc
    29701 ggtcagccct gtaatcccgg cgacacccgg caaatcatca gcagcctggt ggacctggcc
    29761 ttccgggaat atggcgagaa taaccccaga ctttaccgcg caggtacgga acccctggtg
    29821 gatctcgccc tggaagagag cggcatcgct gaacaacatg atgccggatg gtggaatgcc
    29881 gccacctggt ttgaaatcag ggacatgctg catattgcag ggaatatccc cgccgcccag
    29941 cgggcacact accaggccat gcccctgctg gctgaaatgt ccgccctgct gggacagccg
    30001 tccatccggg atgtgttcgg cacggttcag cgtgataact cggaagagcg gttgctggac
    30061 tacatccgcc gtgccctcga tcagggacac agtgactacc cgatgatgtc cggctgtacc
    30121 cggtttatgc tcagccccga cacccgcgtg gtcgccgttg atctgaacaa tgtggccggg
    30181 gacaagacac ctgccggacg tctgcgcacc ggtatcatgt atctgctggc cgggcagatc
    30241 gccggcgggg attttgtcct gccgcagtat caggaggaaa tacggaaaaa tctcgaccca
    30301 cgctaccatg aggtgatttt ccgccgcatt gaacagcttg atcaggaggt caagaccaaa
    30361 gtctatgacg aactgcacaa tgcaaaaggg atcaacttca tctgggaagc cctggacacc
    30421 caggagctgg agcagcgtaa gttcgggata cgcacggtgc tgagcaccca gctgctgcag
    30481 cattatcctc cggccgtgct caaatccgcc aacaccctgt ggctgctgcg ttaccgccct
    30541 gacgaaattc cgttcctgcg ggataacttt ggcgtcccgg aagtcacact gcggcgcttc
    30601 ctgaaaatgc cggaaggggc cgcaccggac ggcagcggtg tcccggtact ggctgtgttc
    30661 cgtgtaaaaa acggcacgct ggcccggatc ctgaaattca ccctcggtcc actggagctg
    30721 tgggcactga actcctctcc taaagacagc gccctgcgac gtgcgctgac gcaggaggtg
    30781 gggtcactgc gggcacgcca gatcctggcc gaacactttc cccggggttc agccacctca
    30841 ctgatagaac accgcgcccg cacacatgac agtgaaaacg tcatccatga gctggcagca
    30901 gaactcatcc gtaagcaggg atataacctc tgatgtacag cattcatatc accccccgct
    30961 gccggcgtcc tgcccgggga caggtcttcc tgaaaacact gcgcgtggac ggtgacagtt
    31021 tcagcttcac cctgccgtcc gcctgcttca cactgcgacc gtcccccaat ccccgggtca
    31081 tttttgccag tgacagacgg gctgacgggg agatgagtgg tattcgcctg gccctgcccg
    31141 gagagacagg cacgctgata acaacggcca cctggcaggt taacgtgccg gagcgaccgc
    31201 cttttgatat acagcatgtc attcactggc ttctgcccgc accactcact gcgctgatgt
    31261 ctgacaacac ctggctgtgg ccggatgtcc ccggcacacc ggcactcaca gccggagact
    31321 ggccggtcat ggaaagcgac accccgacac tgaaacgcgt gaagcgtcgt accttcacgc
    31381 tcatcgataa ccgtaaccgt cagggatgga tcacgcgccg cttccagtgt ttcccgctgg
    31441 ctccgctgcc ggaatcactc agtcatcgcg taccagacat cctgtatccc ccgaaaacgg
    31501 agaaaaaaga atgcaaagac aatctgcaac cacactgctt gcactccttg taggctgtgc
    31561 ggttatccct gccacccatg cctactctgt gaatgttgtg tccagtcagc cggtcacccg
    31621 acagatcatg ccggagctgg ccaccataca gggcatcatg gaggaaatgg tccgtatgca
    31681 gacggccacc ggcacagcca tcacccagaa ctcagaaaaa ctggccaccg tcattgccca
    31741 ggacgggcag gccacccgtc agcagatgat attcagtaat gaaacacacc gcctcgagga
    31801 ggccaggaaa agcttcagcg tgccggactc catctgcagt gaatccgcgt ccggcattgc
    31861 agcagaaagc cgccgggcag cagcgtctgc agcggcacgt ctgagtcagg ggggaggggt
    31921 cagcagtaaa cccatccggg aacgtctctc ccgggctgcg gactccccgg tccgcgaggc
    31981 gtatgatagt gccggcatcc acgccggtta ctgcacggaa gcggagtatg cccgctttgg
    32041 cggaactgat gtctgccctg ccgtgggcga tctgccgggc ggagacagcc aggtacgatc
    32101 gctctatcag ggcgcaggca cagcagatac cccggccgct ctcacctggg atcagaaaca
    32161 gattgatgcc gcgacggctt atatgaaaaa caccgccaga ccgtcagcgg gtcgtgcccc
    32221 ggggaaagga gaagtgggca cacagaccgg gcgcacgtat gtgggtctgc agaatgaata
    32281 caacggcatc atcgatgccg cctctcatcc gcagctgtca ctgattgctg acagtacccc
    32341 taatgaagcc acccgtgggg cactgactga agccctgcag tcaccgtcag ctgcggcata
    32401 ttttgaccgg accgcatcat cagaagcccg cacccgtggc cacatgagcc agcgggagtt
    32461 tgaggccttt gaagccggca gacggtatgc caacaccgac tggcagcagg atctgcaggg
    32521 catggaaggc gataaccttc tccgtgagct gctgcgcacc accgccctgc tgaactggca
    32581 gatgaacgat ctgaaagagc agattcgtca gggtaacgtt atcgccgggc agcaacttgc
    32641 gctggccgca cggcagtatt acggacagcg ccttggcgaa ctgtcacagg ccatgggcca
    32701 ggggagcgta agatgaaaga cgacaacagc atcgacgaaa aaaaaccggc cccccgtctc
    32761 cgccgcatcg gtgatatagg gataaatctg ttcgcaccac tggccgaaac ccgctggatg
    32821 ctgcgcagct gccgccatat gctggaccag cataaaacgc gcctgaagcg cctcatgccc
    32881 gaccggcaac cggagagtcc ggtctgtatg acctgggagg aagcggtcag ggccagcgga
    32941 ctgacggtcg acgaactgga ccggcgtctg cgacgtcgca ggatgttctg gcggacagga
    33001 ttctggctga tgatgatacc ggtacttctg ctgtgtggta tggcccttgc ggccaccgca
    33061 ctgccggtca tcaccctgat acgtatcctg gtctgcctgt tactgatgag taccattgca
    33121 gcactgtgtg cctcaaaagc cctgattgcg tcataccgcc tctggcagct gaatgaacgt
    33181 cgggtcagcg aggacgaaca gggcacattc cccgattacc tgcaggcccg gcacggctgg
    33241 aaacaggcca catggctggc cattacccac cggcagtact gactgtccgg tctgcaattt
    33301 cttttcctca ttccgggggc tgatgcccgg gcatcccctt cggttccgga gttaccgtga
    33361 aaatcatacc cagagcactg agtgcaggtc tggcgctggc tgccgtacct gcacaggctt
    33421 tcgtcaccta tcaggacatt gtgagtgccg ccaccaatcc ggatgacctg tcgcgccagg
    33481 cactggtcac catttttggt gatgtggtca caaatcccct gacagccagc agcaacacca
    33541 tcattggcaa cctgttcatg gtgtttaacg caattatcgc cacgatgggg atagtctggt
    33601 ttctgtttat tggtatccgt catgctgtcc gcacgggaca ccagggcagt gtctttaaca
    33661 acggacggga cgtcgtcggc atactcagtg tcgtgagtgg atttttgatg attgtgccca
    33721 ccgcttccgg ctggagtctg gcgcaactgg tcatgctgtg gggcgcatcc atcatgggca
    33781 tcggttccgc caatatcatg gttcagacag cggcagaaaa cattgctgaa ggctattcaa
    33841 tgaccgtaca gccagttcat gtctccaccc gcacggcagc acggggaatt ctggagatgg
    33901 aactgtgcaa acacgccatg aatacgggcc tcaatgactt taaccgtacc gcacgttcat
    33961 ccacgccgcc catgagcgaa tcctcacgga cagagaatgg cagatatacc gtcaccgtct
    34021 caaatggcag cgccacctgc gggagtgtga gtctctccgt gaaaggaaac ggcagtaaca
    34081 agcagtccgc gctggcccat tttttcaatc ccttcattga aacggaatat cagggggttc
    34141 tgacggcaca gcgctccgcc atggatacca tgctccgtga cctggacagc gcggcgcagg
    34201 aatttgtgtc ggcataccgg acccggcgcg acacgggtaa cggcaacctg ccggatattg
    34261 aaagccgtat tcagaaggca gccaaccggt atgaagaaac ggtgcagaag gcgttgccaa
    34321 cagataacgc agaaactgag cgcagggagc agctgaaatc ctacctgacc acctacggct
    34381 gggttgccct gggcgcctgg taccagacct ttgccaccgc caaccagcga ctggctgaac
    34441 tggccgaccg tgctcctaca gtgagtggcg tctcgtcact gggcgaagcc ggggatacgg
    34501 aattcctgaa ggaagtcaag ggggcttacc aggcacagct acagaactca gcgtatactc
    34561 ctgcactggg aacaaccacc tctgccagtg aataccacgc atctaatact caaaatcctc
    34621 aatcagccct gttagagttc atagggccag ggttaactcg attttcaaat aaagtagcaa
    34681 ccatacattc aggaacagga acaacgagtc agcaggcaaa tcctctgctg aagatgaagg
    34741 acattggtga ttacaccatg gtcacaaccg aaaccatctg ggcgggttat accaccgtca
    34801 gggtgctttc tactgccggc agcaactccg tttttggccg actggtcaat gccgtcagcg
    34861 gcttatctga tgcagcaaag gcttttctgg aagcctccgc accaccactc tacttcctgc
    34921 tgtttttgct gttctgtgcc ggtttttcgt tatctattta cctgccgttt atcccgttta
    34981 tcttctggat gaccggcatc ggcaactgga tagtcagcgt actgattgga tgtgctgctg
    35041 gccccctgtg ggccgccacc catctgggaa caagtcagga caggggaagc cgggccgctt
    35101 acggttacat ctacctgata gacagcatga tcagaccgct gctgatggta ttcgggttct
    35161 tttttgcatc ggtcgccatc gtggccggcg gcacaatact gaatgcgctg tttgctgctg
    35221 cgctgatgaa tgtccagata aactctgtga cgggcgtgtt cagcatggcg ggatttttga
    35281 tgatttatgc ccgagcctgt accacgctgg ttgcggccat ttttgcactt caggcttatc
    35341 tgcctgacca tgtaattaat tttctgggcg ggcgtgacgg tgttaatatg ctgggaaata
    35401 tggtttcttc agtcaaagat attattgccg gaggtaatac caatatgcgc agaacacctg
    35461 gcgttcggga agatagactg aaaaatatta atagagatga taaagacgga ataaaacaat
    35521 aaaatgaaaa catagaaaaa ccaataaaac agcattgcca tattcacctg aagagtttaa
    35581 aatatattca ggtgaatatg gaggtttatt atgaacaaaa caaaaagcag atatccttcc
    35641 tggtgggcat attaccactt ggcccgcaac gcttatttct tattaggaat accgtgttta
    35701 cttttatact cagccgctta tacatcagat ttattagatg ataatctaag acattcaata
    35761 agtcttttta ttctggcata tggatgttat tttcttcttt tcattcctcc gctctggtta
    35821 tatctgagaa ccagagcgaa gaaaaagaaa gtccagcagg ttgttcagca gataaaagaa
    35881 agcagtccct ttgcgccgac cagcgattac gagcaactgt cattctcaaa gagttgttat
    35941 ttcgggatcg acattaaaaa cggcaccatg ctgtatgtgc gcatttatcc gaacaatgtg
    36001 atggatgtga ttggtctgga tattcacaac tttacccgca cggttgctga ggacggcaag
    36061 ctggaaatcc acaccaccta tgtgagtctg ccgatgattc ctcttgagat ctccggcatc
    36121 tcagcaagga cgctggccaa caccatgcac accatggctg cacgtggata tgagtacaag
    36181 gaacgtttcc cacagatgat ccggtatcgg gttaaagagt gggaaaaagt tgccggtgtt
    36241 cctgttgcag aagtattttg aggtagctat caactgttag acgataatcc gtcagccggt
    36301 tttctctgaa ccggcttttt cttttcaggc agaatgcctg tctgccgtct atctcaggac
    36361 gccgcatgga gtctctatgt tttccccaca aattcttagc actgtcttgc ccactgtaga
    36421 caaaatcaat acaattctgc atatcagtta tctataaaag ataacaatat cattataaat
    36481 aattatcata tccacacact caaggcagta cctagaaaac ataaagataa aatcatgtct
    36541 gaatcccgca gcaattttcc agcacgacat ggtactccat ggaatactga agagttacag
    36601 cgtcttctcg tatccatagc ggagggcgtc tcgctaaaaa aacttactca aacacatcaa
    36661 cgatcgtccg ggggaatacg tggagcaatc aaacgcctgc ttccttctga gtttcttgag
    36721 ttcaacgata taaacacggt gaatgatctc agcaattact tcaacaagat tcctcgccaa
    36781 gaacacaata gtatcatcaa taatttaata tcattgagca ctgcaaaaac aattgctcat
    36841 gataaagttg aaatacaaaa gaatagtttt aaacaaaata ccccactccc cctctgccaa
    36901 cccaatacag attttaaaga ttatatagcc gatgaacatg atgtcattat gctaattcat
    36961 tctgctatcg aaagtttatc taataaacga catcaatata ttcttctaat gcgactcggc
    37021 acagatgatt cacctcattc tctggcagat attgccgtca aattaggaat ttcccgtgaa
    37081 agggtaagac aacttcagga acgagccctt ggcttactag ctgctaaatc tcgctatgaa
    37141 gggacacctg gagcttgcct taaagccctt attaactcaa taggtaattc accatatgat
    37201 atagcagtat ggatttatcg tattgttcat cgggatttcg ttacatcctc tgagttagcc
    37261 gccaaattca tcatttttgc cgcaggcatt cctaaagcaa gacgagatga aataaaaaat
    37321 tcgctctcat taatatcaca attacaaaag cagaagttac gtgaacgaag agcagaatta
    37381 gcacacgctc acgctcagga aagagcccga atcaagaaag aaagagttga gttcatattc
    37441 agtaaatggc tagacaatac acattggcca aaatatatcg cacctcctcc ccccgtagaa
    37501 cagctatgtt cacagagagc ggttaatgat actgacatat ctggattctt ttactctcaa
    37561 aaattaggtc gtacagtcca gtacgaatca gggcttgagt ttaaaataat gaatctatta
    37621 gaatgttgcg atcaggttct tttctatcaa gaacaaccat tttctattcc atattgtttt
    37681 aaaaacaaat caaaaaaata ctatccagat ttactcattg cgacagttga tggtcggtgc
    37741 ctacttatgg aagtgaagcc aacagaccga atgaccttga gtctcacgag aataaaaagt
    37801 aatgcaggtc gcaaatgggc acatactcga ggatggggat ggttggtcat tagtgatagg
    37861 ttctcgttac gtcagctgga agaacatgcc atctcaacaa acacatggaa acttatagag
    37921 gctgaactga aaacatcaag gattttagca tggcgtgaaa tgatggagtt acgtacacga
    37981 tacgagattc atcgtttaga tcttattgca tatatcattc agtctggtgc tgaagtggat
    38041 aacttttatc gaatcacccc gaaaatatct aataatacat ctaaccagag acaaccaaat
    38101 gagagcgact gtcataattg atgacgcaca ggacggaaaa aataatagct gccggacagc
    38161 agcaaccagc agtcgtccgg taaaaaagac aaacaaggcg cattgaggtc agccttcgca
    38221 acaatccggc ggcagatgtc ctgccgccgt gttgcccgtt aacgtttaac ttcgtaggct
    38281 aacgttgcca caagcaccgt atttccctgc tcgatacgaa atccgcaaag cgaatccctc
    38341 accatccaga caaaacacag tatcgtcaca cagacgacga ttaacatcat taaaaacgtt
    38401 cgttgtggca tcgcagcccc tccattgcct ttcggccgtc atcgggctac tataatgttg
    38461 ttgaggcatt acagtgggcc ctgggttgat tgataaaaaa atcgcctggg gctttctgct
    38521 acatgccgtc cacaaaacgc tttcggcata cagcctcaag cgcccgccgc tattctaatc
    38581 gttttcctgt ccgccacaat ccttttcatg ccaccacgta ttttgtgtca gggacagaaa
    38641 taaagccgga aagtgactac gtgtgcacac atctgttttc cgggctgcag gcatcgtgtt
    38701 attttctgaa atgcaaaata ataaaaaaca aggacacatc atgaaggatg aaatacacag
    38761 taccagcatt aacccggatg ttgttgagca gtttctggcc cttatgaaca cacattgtaa
    38821 atcctcagaa gaggcactcg ccatgctgag tacgctttgt tacaacctga tgatgacatg
    38881 cggtatccat tccatggaca gccagatgct caatgggcat gggatatcca tgcaacttca
    38941 ggggattgat attgccgaaa ggccaggcaa gaatattcac taacctgaac agggtctgaa
    39001 aacgctgatt tttcaggtct ctgcaggcta aaactgatga tgtaatgaca acaaaatttc
    39061 tgcgtttgta ccccaccgat gaaaaagaca aaaactcaca ccgggcttct catcattaaa
    39121 gataaaaccc gacgtgtcag cctctacgaa acgccaacgg catggtgtat cagggggcag
    39181 gagtgctaca gcaaaagcac tggacgccgc tgtggcagcc acgacagtct gagcagactg
    39241 cgcctggaca gcattaagcc tgttgaatga gtggtggcac catgaaagat aacaacacaa
    39301 ccgccacccc agaagcctgg aaaatgcgtt ttctggccaa cgggacatcc ggcacatggt
    39361 tttacactga cgacattgca gtcactgacg cgtggcttcg cgcgcacccg gatgatgtgg
    39421 aggttatccc tctctgccgg atacagcccc gccagcattc ccatgaacaa catattgaag
    39481 aactccgcgc agccctggcc cggatgctcc atatgcatca tatgatgctg aaaaccacac
    39541 attaccggga aaatatcacc gatgcccgtt gtatccgtga aatgcatgaa gcgccattac
    39601 aggcgctcaa tattctgtcc agatacccgc ataaaaagga cgaggtgtga cgcatgtggc
    39661 tactgatttt ctggctttca tcgcccttta atgtctcttc cggtgatgcc cgtcagccca
    39721 cactggcggt gcacagccag gcctttcata ccgaacgggc ctgccgcgat gcattgcgcg
    39781 ccattaaaaa ggagaatgtt ggcgaatttt ctctgcgggg cgtttgtgtt tctgacggaa
    39841 atacgaagga ggaggctgag agctcatgac ggatattact gacagacata cacttcgccg
    39901 tatgtcctgg tcggaactca tcacggcagg acaggaagca gaatttcagc gtgattacga
    39961 gcgtgccaga atagtgtggt cattcgcctt acatgttgca acaaccacga ttaacaaaaa
    40021 tctggccata gctcatattc gacgctgcga tactttgttg cataaatcaa aggctgtacc
    40081 cggaaacacc ccgtgtggtt ccggtcagcg agtctgagac ctcaacatcc cgggtgatga
    40141 ttgtcattcc ggactgacgc agccgctgca catctgcggc aatacgttga ataccaagcc
    40201 agaacagacc atccagcgcg gtgacaatta acccgctttc cagcgccagt ttcagccgtg
    40261 ctgtcggggc tttaacctgc ccggctatat cagtgaaaca acgcgtcgtc atgccatagc
    40321 tgtccacccg ccgtattcgc gtcgcaagac tgtaactgaa gctatccggc acgccggata
    40381 agtcattctg cagactgtat acacatcccc gctgccgctc attgaatacg acttttttct
    40441 ggctgagctg ccattcccgc cgcacgctgt caatcatctg cggtgtccgg gttaacagca
    40501 gacgaaacgg cagttcaaaa ctggtcacat aatcaacatt aagtaaggca atccgcagcc
    40561 gctccctggc tcctgctccg gtatcacggc attcacgcat gacatcttcc cactggcgca
    40621 tcagtcgcgg tgactcattc ctggccctga actgatattt ctcaatctga taatcaatat
    40681 gttcctgcat accgtcaccg gatatctgtt gccgtatcat cacggatatc agcgtactgg
    40741 aattcatgcc ctgcgtgaag tatcacactg ccaaaaaaac acacctgaac gttattattt
    40801 cgcaatatta attgcacctg ttttcataaa taaaaaggag acgatgaaat gataaaatat
    40861 atcttttcac tcaccattat tttttccgct tctctctgtg ccgcacagat tcaggggcac
    40921 gtcatccgcg ttcttgatgg tgatacgctg gaaatattac aggacaaaaa gcccgtcagg
    40981 gtccgtctgg ctaatattga tgcaccggaa aagaaacagc cctttggacg ctggtcagcc
    41041 actcagctga aggacctggt ggccggaaag cccgtaaccg tgcaatacgc gcagacagac
    41101 aggtatggcc gtattctcgg ccgggtctat accgctgacg gcacagaagc aaatcgctgg
    41161 caggtccgta acggcgctgc ctgggtgtac ccggactaca ataatgacta tactctcccg
    41221 ccgctccagc gtgatgcccg cgccctgaag cggggattat gggcagataa aaatccggta
    41281 ccaccatgga cctggagaaa acagaataag aaataattat ttgctgatat gaagaaaaaa
    41341 accatttcaa agaaaataaa tggcaaaaat acagcatgga aaaaaaattg ccatttttac
    41401 cgccgcgtta tttttaatat ttgctgtctg ctggtcgtaa taagcagaat aacagaaagg
    41461 tgtaaaaatg aacctggttg atgcatttgt cactaaagtg atatccggac cgtatgaaga
    41521 atacggaaaa tggtgggtgg atgtagaata tatttcctgg ggagttccgg gtaaaagccg
    41581 gctcatgttt gactcacggg aacaggcaca ggaagtgcag gaaggttttc gatttttaac
    41641 ctgagcatat tttgttaaag ggcagaaggc ggtgaccctc tgcccttaag tacgtttccg
    41701 ggattatcag tcaaccagtt cccggatcag ttccaggaga gctatcactg cgttagccac
    41761 agcaacaacc gccctgagaa cgatgattaa caggttaatc atccgcttct ccatcaatgg
    41821 agaaagaaca ccgtggtttg cccttacatt gcctgtcgcc acgttttcta tagtgtcagt
    41881 tatagacagt tcttctccgg ccagagcacc aaaaccagca ccacctgatg ttttggccag
    41941 gacttgcaac ctgttcatcc cggtgaacga tatgccggaa agaacgtgcc tcaaagctga
    42001 cacaccgagt atatacttat actgtgcttg cagaaatatt atttcagccg tattatttca
    42061 tagtcagtta taggcagtta tcttccggtc agggctacca acttcgatcc ggaatcgcaa
    42121 aaaaaccggc ctcaccagtc ggtttttttg cgtctgtacc tcctccccgg acagcagttc
    42181 tctcactccc gttgaatatt tcctgttgaa ttttacgaaa atacggcaaa tgggagatat
    42241 cgaatatatt tacttgggtg tagtcggtaa aaaccagctc atgtttgact cacagaaaca
    42301 ggctctggaa gtacagaaag taatatgtcc gtagattatt aaagggcaga aggcggtgac
    42361 cctctgccct taagtacgtt tccgggatta tcagtcaacc agttcccgga tcagttccag
    42421 gattgctatc actgcgttag ccacagcaac aaccgccctg agaacgatga ttaacaggtt
    42481 aattgtcacg aacggtgcaa tagtgatcca cacccaacgc ctgaaatcag atccaggggg
    42541 taatctgctc tcctgattca ggagagctta tggtcacttt tgagacagtt atggaaatta
    42601 aaatcctgca caagcaggga atgagtagcc gggcgattgc cagagaactg gggatctccc
    42661 gcaataccgt taaacgttat ttgcaggcaa aatctgagcc gccaaaatat acgccgcgac
    42721 ctgctgttgc ttcactcctg gatgaatacc gggattatat tcgtcaacgc atcgccgatg
    42781 ctcatcctta caaaatcccg gcaacggtaa tcgctcgcga gatcagagac cagggatatc
    42841 gtggcggaat gaccattctc agggcattca ttcgttctct ctcggttcct caggagcagg
    42901 agcctgccgt tcggttcgaa actgaacccg gacgacagat gcaggttgac tggggcacta
    42961 tgcgtaatgg tcgctcaccg cttcacgtgt tcgttgctgt tctcggatac agccgaatgc
    43021 tgtacatcga attcactgac aatatgcgtt atgacacgct ggagacctgc catcgtaatg
    43081 cgttccgctt ctttggtggt gtgccgcgcg aagtgttgta tgacaatatg aaaactgtgg
    43141 ttctgcaacg tgacgcatat cagaccggtc agcaccggtt ccatccttcg ctgtggcagt
    43201 tcggcaagga gatgggcttc tctccccgac tgtgtcgccc cttcagggca cagactaaag
    43261 gtaaggtgga acggatggtg cagtacaccc gtaacagttt ttacatccca ctaatgactc
    43321 gcctgcgccc gatggggatc actgtcgatg ttgaaacagc caaccgccac ggtctgcgct
    43381 agctgcacga tgtcgctaac caacgaaagc atgaaacaat ccaggcacgt ccctgcgatc
    43441 gctggctcga agagcagcag tccatgctgg cactgcctcc ggagaaaaaa gagtatgacg
    43501 tgcatcttga tgaaaatctg gtgaacttcg acaaacaccc cctgcatcat ccactctcca
    43561 tctacgactc attctgcaga ggagtggcgt gatgatggaa ctgcaacatc aacgactgat
    43621 ggtgctcgcc gggcagttgc aactggaaag ccttataagc gcagcgcctg cgctgtcaca
    43681 acaggcagta gaccaggaat ggagttatat ggacttcctg gagcatctgc ttcatgaaga
    43741 aaaactggca cgtcatcaac gtaaacaggc gatgtatacc cgaatggcag ccttcccggc
    43801 ggtgaaaacg ttcgaagagt atgacttcac attcgccacc ggagcaccgc agaagcaact
    43861 ccagtcgtta cgctcactca gcttcataga acgtaatgaa aatatcgtat tactggggcc
    43921 atcaggtgtg gggaaaaccc atctggcaat agcgatgggc tatgaagcag tccgtgcagg
    43981 tatcaaagtt cgcttcacaa cagcagcaga tctgttactt cagttatcta cggcacaacg
    44041 tcagggccgt tataaaacga cgcttcagcg tggagtaatg gccccccgcc tgctcatcat
    44101 tgatgaaata ggctatctgc cgttcagtca ggaagaagca aagctgttct tccaggtcat
    44161 cgctaaacgt tacgaaaaga gcgcaatgat cctgacatcc aatctgccgt tcgggcagtg
    44221 ggatcaaacg ttcgccggtg atgcagcact gacctcagcg atgctggacc gtatcttaca
    44281 ccactcacat gtcgttcaaa tcaaaggaga aagctatcga ctcagacaga aacgaaaggc
    44341 cggggttata gcagaagcta atcctgagta aaacggtgga tcaatattgg gccgttggtg
    44401 gagatataag tggatcactt ttcatccgtc gttgacagtt aatcatccgt ttctccatca
    44461 atggaggaag tcaccgttgc ttgctcatac atcgcctgtc acaacggctc tctgacattg
    44521 ttggatgtag agtacattcc cccctgccag agcactgaac acagcaccac ctgtttgttc
    44581 agccaggact ttaattcatc catacacaga tagaacaggt tttagaagca agggaggcac
    44641 actgtacagc gccaacacaa agattataca gatgcacaca taaaattcat ttaaagaaat
    44701 gaatattgac aggcaatctg aagtcaaact agacaacaga tgtctggtgc cataatatta
    44761 tggatgttgg atgtagagta tcttcctccg gtcagagcac caactccgct ccggaatcac
    44821 aaaaaaccgg cctcaccagt cggttttttt gtacctgtct cccaccacct tctgccgttc
    44881 gctcttctct gtgaaaatgt cctgttgaac tcattcctgt ccggttatta ccggcgctga
    44941 ttcgcattca tgccacccga cttaatcccg aaatttatcc attcatctat ggaggaatcc
    45001 ccgtgtctta ccaacagacg tctgccgccg aagatccaat ggctatatgg tatatcgtcg
    45061 gtgccatatg cctgctcttt gcgattatca tctggcgctt tttacctgaa atcgtcttcg
    45121 cctcctgcct gatcctgcac acactgtggg gcatgattga ctgggggccc tttcacaact
    45181 ttgccgcccc ccgttacaac ctgctggcca tcactgctaa caacgcggca accatcactt
    45241 tcagccagtg gctggatgtt atgtcccgga cagtggggat cctctggttg attctgcttc
    45301 ccatgacatt cggctttctg tggatgtggt ttcaccatcc ggcccagccc cgttttaccc
    45361 gcagaccact gaatatccac acgctgccac atctgttttc atcactgtca ccggcgatcg
    45421 cccccgttct ggctgacgga gacagcaaca gactcttcca cggacaaaaa cgtcccgagc
    45481 gccgtgtcgc tctaaccccc gaagcctttg tggagcagaa caaccttatc cgcaacatgc
    45541 agctcgatgt tgcggccacg cgccagtgct ttatggcgca actgggccag cctctgacca
    45601 cctggaaaga catggctcca catgaaaaag cactctttgc cgtctttggc ctgcagttct
    45661 tccttgggga ccggaaatcg gctgtttccc tgatgaataa cctcaacctc tcctgcagac
    45721 tcaaaagcaa gcgggaccag gggcgtttca gcacgccagt gtattcactg gccagaaacg
    45781 cctttatccg ggtcatcaaa acagaaggcg cacaacagtg gctcaggcag caccgttacg
    45841 ttcgcagcgg tctggtctgg ctttacgcac atgatttgcg cctgacgcct ccgaactggc
    45901 tgtggctcaa gggcgttgac cggacgctct tctatgccct tcaccgggca aacaccacaa
    45961 aaggcttcat cgaaggggcc ggcgtggtgg ccgttgcccg ggcagaaaat gaagccagcc
    46021 gtctcggcct cccctgcccg gaaccctgtg tggaagaggc cattgaaggg cttcgacggg
    46081 acatgctggg gctgggcctg atatgggacg aacctcagcc agatcgcgat cgtaaacgtc
    46141 agatcaggac ccgctggtca ctcacggatg acgtgatacc ccgccgccat gataacgacg
    46201 aagacactga cactggtgaa accaccgaaa ccaccgaaac caccgaaacc accgaaacca
    46261 ccgaaaccac cgaaaaacag catcccgccg acaaggaaaa agaacaatga ccatccacta
    46321 cactgcaaca ctgaataata aacacctgtc cctgatcgcc ctgtaccctg acggtaatcg
    46381 ggaggtgatt gccatcctgc cagtcacaga actgggcgca ctgattaacc gtaataacag
    46441 gctgatgcac ctgtccttca gttccccttt tcgcgaaata accgaaacgg tgcttgttga
    46501 taccgttgat gcagaacgtc ccggacatca catcggcaga atgctctcag cgattgatgc
    46561 ctgcctggat gccgaatatg ccggaaaagg aaaacatccc tcccggaaaa atggcttctg
    46621 gcggacactg atgtgtggct ttatcgccgg aattgctctg ccggcgacag ttgcaggtta
    46681 ccactggatg aaggcagagc ccccttccac gctggcttac cctacatctc aggaatggag
    46741 agctgaacca gtaaccgaca cagagacacc acgcccacat acggaacatc atgttaacag
    46801 catcccggcc cgcccggctc ccacacagca ggaaccttct tctgcccccg ccccgacggc
    46861 agtgacggaa gaacaacacg ttcaggccgc tgaggcccgc cgtaatctgg ccacagtact
    46921 taaacgcaac gcagaccggg ggatgttcac cattaacctg tccactggtc atgaacgcac
    46981 gctgtacgcc ttccttgacc ccgcatgcat caactgccgt ctgctggaac ccgcgcttaa
    47041 gcaactggcc cgggaattca gcgtggtgat ttatccggtc tccgtgattg gcggggagtc
    47101 cagtacaaag cggactgctc cgctgctgtg tgaaccggac tcacaaaaac gggcgaccgg
    47161 atggcatcag ctgtattccg ctgacagtgg tatggccccc accggcgatg caccggccgc
    47221 acaggatgga cagtgtctga ccgccgcacg tgctgccatc gatgtcaata accaggcttt
    47281 ccgccagttc ggttttggcg gaaccccctg ggtgttatca gataccggct ggcacctgcc
    47341 gtccggcatc ctgaaagacg ccacttccgt tcgcctcttc ctgaaagcaa cagatagcga
    47401 ggagtatcac aatgagccag caccacgtta atcctgacct catacaccgc acggcctggg
    47461 gaaacccgtt gtggaatgca ctgcataacc tgaacatcac tggtctgtgt ctggccggca
    47521 gtatcattac ggcgctgata tggccactgg cccttcctgt ctgcctgctg ttcaccctgg
    47581 tcacctccgt gatattcatg ctgcaacgct ggcgatgccc cctgcgtatg cccatgacac
    47641 tcagccttga tgacccctcc caggacagaa aggtcaggag aagcctcttc agcttctggc
    47701 ccaccctgtt tcagtatgaa gcggatgaaa cattccccgc ccgggggatt ttttatgtcg
    47761 gttaccgccg tatcaacgat atcggtcgtg agctctggct gagcatggat gacctcaccc
    47821 ggcatgtcat gttttttgcc accaccggtg gcggtaaaac agaaaccacc tttgcctggc
    47881 tgctcaaccc gctctgctgg ggaagaggct ttacctttgt tgacggaaag gcacagaacg
    47941 ataccacgag gacaatctgg tatctctccc gacgttttgg cagggaggat gatatcgaag
    48001 tcatcaactt catgaacggc ggaaaatccc gcagtgaaat cattcagagc ggcgaaaaat
    48061 cccgcccaca gtcaaacacc tggaacccgt tcgcattcag caccgaagcc ttcaccgccg
    48121 aaaccatgca gtccatgctg ccccagaacg ttcagggcgg tgaatggcag tcacgggcga
    48181 tagccatgaa caaggccctg gtctttggca caaaattctg gtgcgtccgg gaaggtaaaa
    48241 ccatgtccct gcagatgtta cgggaacata tgacgctgga agggatggca aaactctatt
    48301 gccgtggcct tgatgaccag tggccggaag aagctatcgc accactgcga aactacctgc
    48361 aggatgtccc gggattcgat atgtcactgg tgagaacgcc gtcagcctgg actgaggagc
    48421 cccgtaaaca gcatgcttat ctctccggtc agttctcgga aacgttcaca acctttgccg
    48481 aaaccttcgg ggacgtcttt gcggcagatg ccggcgacat cgatattcgc gacagtattc
    48541 acagcgaccg cattctgatt gtgatgatcc ctgcactgga cacatcggct cataccacgt
    48601 cggcactcgg tcgtatgttc gtcacccagc agagcatgct cctcgcccgt gacctgggct
    48661 accggctgga aggcacagat gcacagaccc tggaagtcaa aaaatacaaa ggcagcttcc
    48721 cctatatctg tatccttgac gaagtcggtg cctactacac agaccgcatt gctgtggagg
    48781 ccacccaggt acgttcactg gagttttccc tgataatgac ggcgcaggat caggagcgaa
    48841 ttgaagggca gacctccgcc accaacaccg ccacactgat gcagaacgcc ggcaccaaat
    48901 tcgccggacg cattgtcagt gacgacaaaa cggcaagaac ggtgaaaaat gccgccggtg
    48961 aagaggcacg ggccagaatg ggcagcctgc agcgccacga tggtgtgatg ggtgagtcct
    49021 gggtggacgg taaccagatc accatccaga tggaaagcaa aatcgacgtg caggatttga
    49081 tcagactgaa tgctggtgag ttctttaccg ttttccaggg ggatgtggtg ccgtcagcct
    49141 ctgtgtatat tccggacagc gagaaaagtt gcgacagtga tccggtggtc atcaaccgct
    49201 atatttccgt tgaagccccc cggctggagc gtctccgccg tctcgtcccc aggacagtgc
    49261 agcgcaggct ccccacaccg gagcatgtca gcagcatcat cggtgtactg acagcaaaac
    49321 catcccgtaa acgtcgtaaa aaccgtacag agccttaccg tatcattgat acattccagc
    49381 gccagttggc aacagcgcag acatcactgg atctgcttcc gcaatatgat acagatatcg
    49441 aatcccgggc aaacgaactg tggaaaaagg ctgtgcacac tatcaataac acaaccaggg
    49501 aagaacgccg ggtatgttat atcacactga atcgtccgga agaggaacac tccggtcctg
    49561 aagatatacc ttcagtgaag gcgattctga atactctgct ccccctggag atgctgttac
    49621 ctgtaccgga tctaactgca tccccgcccc ataaaaaaaa tgtcgctcag acaccatcag
    49681 gtggaaaaca agagagtcgt aaaaaaagat tctgataaca cctgtatggc tgattttaat
    49741 aacaaccttt acgcgattat taatctcctc cgctcaccat agtaagcgga ggattcagga
    49801 tttcaatcca tcaccagagg tacaataaat gtgtattagt agcccggaaa caaactcatg
    49861 gtctgttatt tatcgtaaaa attccggaga agatatcaat atcacttccc tgacgtttaa
    49921 aaatagtctt ctggcagccc ggacactgat ggtgcccgaa aactatatga tatgcatact
    49981 cagaaacggg gagagggtca gacgatggga cagagaaata cttgcaggct caaacagatg
    50041 gtacaagtgt tctcccgata atttcgaaat actgggtaag ctccccataa taaataaagt
    50101 cacaaaactg ataaaatcat aatctacgga ttttaatcag ttaagctaaa tcaggatgaa
    50161 cgctttgatt atgaattata catgaaaaca aatatgggca gcacaaaaaa tggtgccagg
    50221 gttccccacg ttcgtaatag attcacttct atggatattg gtatgaattc ccagatccca
    50281 ctctattgta aacaagacat ttttatcttt tatattcaat ggcttatttt cctgctaatt
    50341 ggtaatacca tgaaaaatac catgctcaga aaaggcttaa caatattttg aaaaattgcc
    50401 tactgagcgc tgccgcacag ctccataggc cgctttcctg gctttgcttc cagatgtatg
    50461 ctcttctgct cctgcagcta atggatcacc gcaaacaggt tactcgcctg gggattccct
    50521 ttcgacccga gcatccgtat gatactcatg ctcgattatt attattatag aagcccccat
    50581 gaataaatcg ctcatcattt tcggcatcgt caacataacc tcggacagtt tctccgatgg
    50641 aggccggtat ctggcgccag acgcagccat tgcgcaggcg cgtaagctga tggccgaggg
    50701 ggcagatgtg atcgacctcg gtccggcatc cagcaatccc gacgccgcgc ctgtttcgtc
    50761 cgacacagaa atcgcgcgta tcgcgccggt gctggacgcg ctcaaggcag atggcattcc
    50821 cgtctcgctc gacagttatc aacccgcgac gcaagcctat gccttgtcgc gtggtgtggc
    50881 ctatctcaat gatattcgcg gttttccaga cgctgcgttc tatccgcaat tggcgaaatc
    50941 atctgccaaa ctcgtcgtta tgcattcggt gcaagacggg caggcagatc ggcgcgaggc
    51001 acccgctggc gacatcatgg atcacattgc ggcgttcttt gacgcgcgca tcgcggcgct
    51061 gacgggtgcc ggtatcaaac gcaaccgcct tgtccttgat cccggcatgg ggttttttct
    51121 gggggctgct cccgaaacct cgctctcggt gctggcgcgg ttcgatgaat tgcggctgcg
    51181 cttcgatttg ccggtgcttc tgtctgtttc gcgcaaatcc tttctgcgcg cgctcacagg
    51241 ccgtggtccg ggggatgtcg gggccgcgac actcgctgca gagcttgccg ccgccgcagg
    51301 tggagctgac ttcatccgca cacacgagcc gcgccccttg cgcgacgggc tggcggtatt
    51361 ggcggcgctg aaagaaaccg caagaattcg ttaactgcac attcgggata tttctctata
    51421 ttcgcgcttc atcagaaaac tgaaggaacc tccattgaat cgaactaata ttttttttgg
    51481 tgaatcgcat tctgactggt tgcctgtcag aggcggagaa tctggtgatt ttgtttttcg
    51541 acgtggtgac gggcatgcct tcgcgaaaat cgcacctgct tcccgccgcg gtgagctcgc
    51601 tggagagcgt gaccgcctca tttggctcaa aggtcgaggt gtggcttgcc ccgaggtcat
    51661 caactggcag gaggaacagg agggtgcatg cttggtgata acggcaattc cgggagtacc
    51721 ggcggctgat ctgtctggag cggatttgct caaagcgtgg ccgtcaatgg ggcagcaact
    51781 tggcgctgtt cacagcctat cggttgatca atgtccgttt gagcgcaggc tgtcgcgaat
    51841 gttcggacgc gccgttgatg tggtgtcccg caatgccgtc aatcccgact tcttaccgga
    51901 cgaggacaag agtacgccgc agctcgatct tttggctcgt gtcgaacgag agctaccggt
    51961 gcggctcgac caagagcgca ccgatatggt tgtttgccat ggtgatccct gcatgccgaa
    52021 cttcatggtg gaccctaaaa ctcttcaatg cacgggtctg atcgaccttg ggcggctcgg
    52081 aacagcagat cgctatgccg atttggcact catgattgct aacgccgaag agaactgggc
    52141 agcgccagat gaagcagagc gcgccttcgc tgtcctattc aatgtattgg ggatcgaagc
    52201 ccccgaccgc gaacgccttg ccttctatct gcgattggac cctctgactt ggggttgatg
    52261 ttcatgccgc ctgtttttcc tgctcattgg cacgtttcgc aacctgttct cattgcggac
    52321 accttttcca gcctcgtttg gaaagtttca ttgccagacg ggactcctgc aatcgtcaag
    52381 ggattgaaac ctatagaaga cattgctgat gaactgcgcg gggccgacta tctggtatgg
    52441 cgcaatggga ggggagcagt ccggttgctc ggtcgtgaga acaatctgat gttgctcgaa
    52501 tatgccgggg agcgaatgct ctctcacatc gttgccgagc acggcgacta ccaggcgacc
    52561 gaaattgcag cggaactaat ggcgaagctg tatgccgcat ctgaggaacc cctgccttct
    52621 gcccttctcc cgatccggga tcgctttgca gctttgtttc agcgggcgcg cgatgatcaa
    52681 aacgcaggtt gtcaaactga ctacgtccac gcggcgatta tagccgatca aatgatgagc
    52741 aatgcctcgg aactgcgtgg gctacatggc gatctgcatc atgaaaacat catgttctcc
    52801 agtcgcggct ggctggtgat agatcccgtc ggtctggtcg gtgaagtggg ctttggcgcc
    52861 gccaatatgt tctacgatcc ggctgacaga gacgaccttt gtctcgatcc tagacgcatt
    52921 gcacagatgg cggacgcatt ctctcgtgcg ctggacgtcg atccgcgtcg cctgctcgac
    52981 caggcgtacg cttatgggtg cctttccgca gcttggaacg cggatggaga agaggagcaa
    53041 cgcgatctag ctatcgcggc cgcgatcaag caggtgcgac agacgtcata ctagatatca
    53101 agcgacttct cctatcccct gggaacacat caatctcacc ggagaatatc gctggccaaa
    53161 gccttagcgt aggattccgc cccttcccgc aaacgacccc aaacaggaaa cgcagctgaa
    53221 acgggaagct caacacccac tgacgcatgg gttgttcagg cagtacttca tcaaccagca
    53281 aggcggcact ttcggccatc cgccgcgccc cacagctcgg gcagaaaccg cgacgcttac
    53341 agctgaaagc gaccaggtgc tcggcgtggc aagactcgca gcgaacccgt agaaagccat
    53401 gctccagccg cccgcattgg agaaattctt caaattcccg ttgcacatag cccggcaatt
    53461 ccttttcctg ccagacatgc tgattaattt tgcccttatt aacatcacag acagaaagaa
    53521 cgagggtacc aataccattg atggtaactg gcaggccgat gaaggacgcc gttaccggga
    53581 taatgtaaga atctattttt gagtatgtca acataacctg aggcaaagta cctcaggttc
    53641 cagtgtagta atcagccaac ctgacagtct tctgttagcc ctacagatat gtcgtggagc
    53701 taaccgcgca tgtcaaagcc ggatttttga aaatctctat aaacttccgg attgttgaag
    53761 gccgggaatt tagctttatg ggctgctgac tttatcgctt cgcaataggc tttatcgcca
    53821 ttactggttg atatttttaa cgccgtgcca tcccgggaga attccatatg caacctgcat
    53881 tttttccttt tccatttttg cggatcatca agtttagcat taattgcatc cctgattccc
    53941 cgtgcttgct ccccccattc atcctgatca tcccaacgtc ctgaactgca actacccagg
    54001 gctgtagttt tatggcaatt tgaaggatgt aatggcgcac atcccgccac aaagctgact
    54061 aaaatagcca gcataacgat tttctttacc cccatatatt gctcctcaat ccatatagat
    54121 ctcagtacca tagctctgtc agagcaaaga aaatcttaac taatacaagt taagattttt
    54181 aatatccact cctcatccct acattccagg tgatggtggc gtccactcat tatcctgaac
    54241 tggagcataa gatgcgccat gccccccgga tatgcttttc tgttgcctga tatattcgtg
    54301 gtagtactgt tcactacgct gacgatgtaa atccacatcc gggcggctga ttacaaagtg
    54361 tccacttccg gttattccag ctgcattatg ccaggccacc atttccgcaa atacatcatt
    54421 aaactgttga tatgagcctt ccggttcaaa acccattctt ggctcacgat caaacaatac
    54481 aggagcaatc agattcagct tatccaccag ttcatttctg tcctgatgat ggtagaacac
    54541 cacacggtcc ccataatcca cacagactaa ctcgccattc ctccgatcac gaaaacgcac
    54601 actgccatct ttctgcagac gggcttcgag aacgccagtg tcttcataaa gtggagttcc
    54661 tcccggttca cagagaatca cgcagcggtc ggcattcgtt gttcgtcgct tgtctttgcg
    54721 acgatcacga taatcccagc cacgtaactg tgataatgca gctctgtctc cctgtaccgc
    54781 ctgttgctcc acccactgcc ggtaggacaa aggataccac ttaccctccg caatcagtga
    54841 caaccgctct tccttcacac tctccttcag cctgatcagc gcctgcatac gctgcacttc
    54901 tgcaatatgg taatgcagct tacgcaattg tggatcccgg aactgcacac ggatatacgc
    54961 cttgcgccga cggcaggcag catgaatttc ccgtaaccgt tctccgtaac gtaagtcggg
    55021 tttacgccag tgctccttcc atgcaagata acgcgcgcgc aaatcttccc gcgcagctgc
    55081 acgggcttca cggcgttcac gacgcagtac aggatcgcgc cggaagccga gctccacttc
    55141 atcgctggca gcaagttccg gattgtagcg acattcaggc ttcacccgtt caaatatatc
    55201 tgcacctgca atctcaaatg gccctgcctg cggctcagcc ctggataacg tcaaatccgg
    55261 gtggatactg ctggccttca ctggtgtgag ctcatgatta aacgcatcca caatcacaag
    55321 accatgatgc tgtttctgca acataagccc tttacgcgca aacatctcat ggcacgcctg
    55381 ccagctgaaa ttcccgcccg cgatcatatc ttcaatcaac gactcacact gaacagtcgt
    55441 gtacccccac agactttcct tatcggaaaa cagttcgcgc tttattgctc cctgtggacc
    55501 tgtccccatc cgaatacgat accattcgta atcactgaca gcctgagtac caagattacg
    55561 tccccggaat cgttctccgg gtataacctg agtaaaaata tccttcggta caggctgcca
    55621 tccaccatcc agatcctgca tataatccag cgtcattaac ggccacacac tgtctgcacg
    55681 tactggtgtc tgttgtttat caaatccatc atgcagtacc agatgcccgt gctggatccc
    55741 catccataat ccagcctttg ccagagttct gtgcacatcc aggcaacttt ccagctcacc
    55801 atttctcgcc atttccggta agcgttcggc aaaatgacgg caggcgtact gtttcagact
    55861 ctccgtttct gccacttttt ccggccggat atcaacgttt atggcttccg gatcataacg
    55921 accgggtgtc cccacctgac tgaaaatatc tgtcggtaca ggctgatatt cccccagttt
    55981 ccgccccagt ttttcagctg tccatgacgg tccgaacgaa ctgagtgcaa caccttcccg
    56041 cgctctgtcc cagccatcct tcaccaccag ttccccttcc tgcatcgtga tatacagacc
    56101 atcactggcc agccgtttat gcaaagacaa ccagtcttgt gcgggctctt cacgcagccc
    56161 ggcaatcgac atctgcgcaa tatattccct gaacgtctgc tttttcccgc gtcgccaggc
    56221 attacggcgc tcacgtacca gcgctgtttt gcgcacaata cggttgccgg gtgcatgcac
    56281 aaagcagcca ttatcaggcg cgaatccgtg cttaagttca agttcacgac atgcacggct
    56341 gagtttttcc tggctccagg aaagccagtt aatataacca gtttcaggat gtacacgatt
    56401 cacggcaaca tgcacatgca gattatccgt gtccgtatga acggcagcca cgtactgatg
    56461 ttttgagagt tcaagagatt tcagtgtgtg acgcacacag tcaaaaagct gctcaggacg
    56521 cgggctttca tgcgcaggcc aggaaagaat gtagtggaaa acggggtctg tatcattacg
    56581 ggaaaaaaca gctttgtccg ccttgtactg catctcttca gcagctgttt ccagtgaatt
    56641 gcagttatgg aaacaggtta cgccatagaa atttacccac tgactgccgt ccttcatcac
    56701 atcaatcagg gagacaaatt tttcgttacg caggcgtgtt gcataatcaa caagccgtct
    56761 gaaacggttg cggtgaggga catcactctt gatttcgctg tcttctgtga gctcattttc
    56821 ctgcacatca tcccgcacag acgtgtacgc aatcaggtct tcaaaggaag acctgccgtc
    56881 acgtcttttg tgcggaataa tggcattcat cagcagttat ctcagcgtcc cattaagtct
    56941 cttcgcagag cattgacata ttcagtaatg gcaacaagaa cctctgcata ctccttatca
    57001 ccagtgcgtt ttccctccac aaacagatgc ttctgaaggc ggccaagacg cataatctca
    57061 tcaatcatac ggtcgtcagt acggcagtta accaccttat ccagtgaaga acttcgtata
    57121 aatgttgaaa ccgtctgtcc acttgactcc gctttttctc ttacgatttc agcctcatcc
    57181 tccgtgaatc tcgccgtgaa ttttacaatt ctcttcctgt tctcactgcc cgttcgggtt
    57241 ttcttctcac tcatggtctg ctccttaccg gggtgtcggg gcaaagccct gaccaggtgg
    57301 ggaatgtctg agtgcgcgtg cgcggtccga cattcccaca tcctgtcccg tttttcagtc
    57361 cattatagcc tgttattttt gtgcatcata gtcttatgca gacggcagag gcagggcgca
    57421 gccctgccag gagtcagttt ttgatgataa aatcaggctt caacattaaa cggagagaaa
    57481 gtgccaatgg cgtctaaaaa attttactca gatgatgaca tccagctggc aaaagctgct
    57541 ttatcggaat tgccagacct caccgctcaa cggaaaacat tgcgtgattt tcttgatgcc
    57601 attcgtgatg acatcattat cctggtaagg acaaagggtt atacactgac agatgttcgt
    57661 gacacgctca aaaatgcagg atatgaagca ggagagaaag cacttcgtga cattattcgg
    57721 gaagcagaat cgaaaaaacc atcccgccgt tcttcctcta aaacagcacc taaaaagaca
    57781 gactcaggca ggaaagatgg catcgatatg aacaacaatt gatgtgacaa tgtcacattg
    57841 tgttctgaga ttatgatgat tacagaaaaa aaagccccgg cataaatacc tgggcttttc
    57901 tgtttaatca gactcgtggc ggcaatgcac caggataatg ccgtgcaata atatcattca
    57961 gtttttcaac aaggtcagaa cgcttaaaga caatgtgtac ggtccctttc tggtagccac
    58021 gcaccgtaaa atattcccct tctaacagct taccgacatt gtcaccgttc ccgagaaatt
    58081 caccatactg cgccccgatg ctgttcctga agtcaggaac gttgcgcccc tccagcagat
    58141 aaaacggacg tactaggtca tccagtgcct cccgtccgct gcagtccagc gtcacacgcc
    58201 cgttactcca gcggtacagt aggttttcaa taattatttt cttacccaga cggcagggat
    58261 tattggttct gtaatcccat gacaatttcc ggtagacatc aatcagcccc tgttcaaacg
    58321 tctgcatctt gctggcatta agatggcgga atgttgccag tacattgtcc agggtgattt
    58381 caggacaggt gtcgctcatc agctggctgt tccactcatc acgctggcat gaactcatga
    58441 aggtgtacat gcccgtatct gtcatcaggc ggtcccagat tttccggtcg gcaagacggg
    58501 tgatattcct tttcgcttct tccgtcttat ccatgcgacg aacggaatca gtcacgcagt
    58561 cttcaaaccc gtaaagaaaa atatcacctg acgcatcgag cagaatcttg cgcgctgtcg
    58621 ccaggcattc catcgcctgc atgaacagtg caataccttc gttacgctgc gcgatgatgc
    58681 ggtcgatagc tacagacgga ataacatctg tcgtgccggc agtattcagg tcagcagtgg
    58741 gtaaagtctg attcatcaca ttgtctcctc atcgtcacga ctgccgggtc agccaggtca
    58801 tttcccggag gagcgaagct ccttctgccg tccgggtctt cggggatttc cccgcagtgg
    58861 acctttccga cgggggacgc tcaccacgtc acggggcagt tgtccggggg cagacgcaaa
    58921 gtttttgacg cctgcggcaa aaagttttgt ggctggtaca tctctcccct ggacgactga
    58981 aacgggatgt ggtcatgtat gtacccccgg aaagtccgtc tgcggagaac aggccgcagt
    59041 gcggcggcag acaggcagag acagctgcgc catcaggcgc agtggcctta cacaccgtaa
    59101 ggtgtgcagg gagcgccgca tacggcgcga ctgaagaaaa acgacggcac aacaatggca
    59161 tgtggtggtc actggcacac cactcccgca caggtgaaaa cagagaaaac cgcacgacat
    59221 ttacaaccaa aaaaaagtga aatttcttca ctttttcacg cttatttttc gcgttgattt
    59281 ggtgattatg atgataaaat aaagtgaaat tttttcactt tttcaaaggt gttttatgac
    59341 cggctgggaa ctgcgaatct ggcgaaaaag catgctctgg tcacgcgaaa aagcggccag
    59401 agaatttggc gtgacgcaga gaacatggca cgcctgggaa aatgcggaac aggtggacgt
    59461 caccgtctgg aggaccaccc aggcactttc agtccgtgac ctgctgcctc acatgcaggg
    59521 gatgcgtaag gcagatatca tcaggcgact ggaaaatgaa ctgggggaaa cggcagggaa
    59581 tgtgtgagcg tgattcacag cctggctgaa tgccaggcta ctgctcatca ggaaacagct
    59641 ttttcagttc ttcaccggaa agtcccgtga cctcctgaat ccgttccagc ggccagccta
    59701 tatcccgaag ccggcaggct atttcccgcg ccacttcaag agccccttgt ttaaaaccct
    59761 cgtcgaaacc tttctgtata agacgttctg caatcgtcat gctgtttgct ctgctcctgc
    59821 tgacaaaaaa gcccggcatt ttgccgggct ttgtggttct catcagtgag tgattttctt
    59881 cagttcgtca tcggaaagtc cggtagcctg cttaatctgc tccggcgtca tccccatttt
    59941 ctgaagctgg cacgcaatag ccagggcttt ttcaagcgct ccttccatgc gtccttccat
    60001 gcgtccttcc atcctgcctt cctggcgacc tttttgctca agacgttctg cgatagtcat
    60061 cagtttatcc tcatgctgcg gcagacgctc agccagcgcc cgcataaatt ccggtgatac
    60121 agagtcccct gcgttaacga tgtaatgaat cagaacacgc acctgctcgt ctgaaatttc
    60181 caccatgacc tgcgggagtt tgtccagcag ctccatcatg tcacgatggc ggatatgctt
    60241 catcagcagc gtcagtgcgg ccatccggcg atggttcatg atttcattgt catcaacgac
    60301 agtgatatca accagcggaa acggctttgt gtatatttta gccgcaagtg ccggattctc
    60361 aaaacagtcc agccagttca tgctgtacgg gtacgggctg cgctcaccgt gatagaacag
    60421 caccggaatc accagtggca acgttttgtg ctgctccaga tgtctctgca tcgccgccac
    60481 actgtagcgt aacatgcgaa aaggcatgta cagattttct gtgctctggt gctcgatgag
    60541 cgtataaaca taaccatctt cacccccggt ggttttcacg ctccagagga tatcgctggc
    60601 gtactgatgc aggtccggct caacaaaggt ggcgggttcc agcttcagcg tggacaggtc
    60661 gcacaactgc cggtactccg caggaagatg cagttccaga aaatccctgg caacgtcggg
    60721 attcgccagg aacgaccgga acgcggcatc atgcggcgtg ggcgttgtgg tgtttttctt
    60781 cttactcacg gttcctccat caaatcagca gcatcgcagc aatcatcgcc caaatcccca
    60841 caaccgagag gacagcaatt gcgttcaaag ccatgacaac aatagccgcg ttctgagtca
    60901 cggcgtattt cttcagcaat ctcttctgcg gaaaggtaat caaactcctc acgggccagt
    60961 tccaggacca gtagatgttg ttctgtcggc aatgtggcca caaaattatc aatagcagca
    61021 ctcaaacgtc gctgatatag ctcataaaca agaccacaac ggggcattag ttcttcggat
    61081 aatagttcca gttttgagcg aattctgtca gcgttgaaag tgacttcatc aaaagacata
    61141 tgaaaagctt tgtcagtatc aaaacaagac ttcattgtta tctccagccc tcccgggccc
    61201 tgaatgaaag agaatctgtt tcccttttct gtttttttgt ggagacacca tgcaacacag
    61261 acgtcgtgat gtttttcatc ttgctcacgg ttcctcctgg taaagtacct gaacacatcc
    61321 tgccacctgc acaggcagac ggcaggggtt aatcagtgac agacccgttc aatttcaagc
    61381 gcgcggagca ggtggcccca ttcgctgact ttcatagcgg ggatacgtac ccgcttctgc
    61441 tcactgcagc gtctggcgat atgaccgccc atcgccagga cgtccgggtt aatctcatcc
    61501 ggtgccagtt cctggcgtct gaagcgggta tggcggtgca ggggtttgtt ctcccgcagg
    61561 cactcttcac gcagacgctt tacatactcg gcggttgcga agataattga tggtctcatg
    61621 gcataatctc catagttagt gaatgagatg ccggacttcc gtacggggaa gcgccagcgg
    61681 agaaaggcag ggtttatcgc cctgcttttt ttcattccgg acagcgacgc agtgccagcg
    61741 cgagcgcttt catcttccag ccttccggcg tgcggtacat caccttccag tagccgtcga
    61801 tgaacacata atggaagcgc acctcttcag tgctttcagt ggcaatagcc tccctgaaag
    61861 aacggtactc acgcgcctca ctgtccatcc ggaaaccact gtcacgtccg taataagccg
    61921 tcacaccgtc caccgggttc tgaagcgtat gcccggcggg tttgtcgcaa cggggaccga
    61981 ggcaacggat atcaccgtgt tcaaccaggg cttcggcctg ctgaccggtg gcatagtggg
    62041 tatgcaggat gcgaccgttc caggtcggct caccgtgcag gtggcagtaa acggtacgga
    62101 acaccccgtc gcggcaaaaa acggaaatcg tgctgctgac tgtcatgaaa acgtctcctt
    62161 ctgctgaatc aggtcgccgg gccgaccggt tgctcccccg gaggggcgaa agccccttct
    62221 gccgtccact tcttcggggt ttctccccgc agtggacctt tccgatgggg cagcgacagc
    62281 cacggcacgg ggcagttgtc cgggggcaga cgcaaaagtt tttgacgctt gcggcaaaaa
    62341 gttttgtggc tggtacatct cccccctgga cggctgacac gtgttgtggc cgcttcagcc
    62401 cccggaaagt ccacagcggg aaacaggccg caggggtggc ggcggaaggg cagacaactg
    62461 cgccatcagg cgcagtttcc ttacacaccg tcaggtgtgc agggagcgca ggtcacggcg
    62521 cgacagaggc ggcgcgaccg gaacggtcgc tgcaagcctg gtttttcaga catcggaccg
    62581 tagcggcagc ccgaaaccgc gccagcgggt tcggcggaga ctggcgggtg cgccagcagc
    62641 tgcctgtcgg agtcgggcct gacgaccgga tgccgccagg cagccgggag ccgaagggtg
    62701 tccccgcccc cgggagtcag aacaacagaa acgacaaaac agaaaaatgc ctcacactat
    62761 gacaggcagg aagggcagcg ccgcaggcgc ggccctcctg tatttcaggc aggcgtaagc
    62821 ctgccagggt tttccggggg cctggcaggc catacccccg ccggagcggg ggagtaaatc
    62881 accgacggaa cacatgaccg tcaataaagg tgaaggagtc caggaataaa tcccgcgcat
    62941 acgcctcata gtcaaaatag agcgccaccg tctccggcac gtcagccagc aggccggtat
    63001 cactggcgaa ctcaaccgca aaagcctcct cactgtcagc ctcgccccac caggcatcgc
    63061 ggaaggtgtc aaaatccgtc tcaccggtat cctccaccca gagacgataa gcctcttcgc
    63121 agccttcatc ccgcgcctgg cggaagcctt caacccaggc ccagttgata tggcattcag
    63181 aggccatatt ccccgggaat ccctcataat cctgaaacat cagttccggg tcggtttcat
    63241 cctggtgaag ggcacggcag gcggcgaaaa agtcgcgttc atcatcaaac gttgtcaggt
    63301 caaaccagcg gccagcgatg cttccacagt tgtatttgtg ccaggttcca acgtatacag
    63361 caggtgcaac aacagacatt cccgaatcct ccatagtcac atgttctgcg acaccgggcg
    63421 gtgccgtgcc tccatctttg ccgggcctgc agggttgcaa gggcgcaagg gaagccgcag
    63481 cctgacgcac acggctggcg ggagttggag ggtgagaccg cgaaatctga gcgggtgaac
    63541 gaccggaaaa tcacggcaga cgggaaggag ggaccgggtg accgcagcgg ttcaccttac
    63601 cttgcaaccc gcccgcagaa gcccggctac gatggaggca cggcacagcc cgcacacgat
    63661 gctgaggcca cataactgaa aacccacaag gccatgcccc gcatggccgg gtaaaagcgg
    63721 agccggcggc gacgtcctta ttccggcgag agcagggagc acaccgcatg atctccagac
    63781 cacccctgga acgaaacgat catgaccggc atgtccctgc gtaaaatata catgcctggt
    63841 tcaccggaga atatctgtga tatccacaag ttttctgtcg cgcagccagt cctgcgcttc
    63901 acgcaccaca caactgtccg gtttcaccgc tttcagcgat gatgccagtc gcttgatcaa
    63961 catcaccagt tcccgtgtct gtatgtccgg cattgtctcc ggcggagcag ggggactctc
    64021 atttccgacg ctcatcccat gcgcttccag tgtggcacgg acaaaccagg cggcatcgac
    64081 cagttgctgt ctaatccagg ccagatcggc atttctgtca cgggctgcca ttcgctcgcc
    64141 cgacagcgcc ttgctgatat ggctgcgcac cagattatcc aggagtgcct gcgcttcttc
    64201 cggtgaaaag ccacctggta tctctgtgcg caacagccct ttcctgtaca gaagctgcag
    64261 cagatcaacc tgcgaccgca cagcaactat cagtacataa cggacaacat tcgcactggc
    64321 agtggtttct gtatcattca gcagttcttc cggatcgttc cccccggtgt tgcagagcaa
    64381 cggcaacaga ggtcgcatcc acgcgggaac ctggtatttt tcgtctgatt tcatcatatt
    64441 acgttctcca gcttgttcgg gatcatccac tgctcaagtg cacgtacctg taccggtgca
    64501 ccacgaccgg cctcaccggc ttcaaaaatc agccgccaga gtggttcctc ctgccagaaa
    64561 aaccggtcaa atgatgacag tgatggaaaa cgcccgcacc acccccagaa aatcgtttcc
    64621 agatcatgct cctcaacaac accagaccag cgggacaacc agcttccgac cgtggcggga
    64681 gtatgaaacg caaggtcaat ctgcgcctga gcaaaacatg tctggtattc cagttcccgt
    64741 cgttctgcct cacgttcccg ctggcggctg tatttctgct ctgtcagcgt ggttttctga
    64801 cgacgacgct caccttcacg aaaaggcacg gacgggaaca gacggtgccc cgcatcggat
    64861 ggcaggggaa gaacacagac ttccccaaaa ctttcaatca gtttatccac ggcagccagc
    64921 cacaggagac ggttgccatg aaattcctcc ttcgtcagcg caggggcaaa aaagcggaga
    64981 tcggtcagcg aaatccggcg actgccattc aggcagcgaa agaaagcggt ggcataaggc
    65041 catgctgaaa gtgtgcgtcc ctgacgacga cgttgttctg tgacagccac cgcctgcatg
    65101 gcggcctgtt gttctgcgct gagggggatc agtgcccgtg aacgtgcgct catgcctgac
    65161 ctccttctgc catcaggata tggatgatgt cgttaacact atatccctgc gcatccagtg
    65221 tcgcggcaag cgccagcgtg tgactgatcg cgacaggcgt gaagatatgt tgcacctgaa
    65281 cgacggcgaa aggctgtcca ccaccattca ccagaaccag catccagagc tgagagacgt
    65341 ctcccggcga acataatgcc aggtggaaac gttcgtgggc tggcgtgaaa cgaacctgga
    65401 cggggaaaaa gcccccgaac tcgctgccat actctccgtt catcatccag ttagccggtg
    65461 cgtccacttc acgcatcacc tcgtgagtga gattacggcg catatcgctt cccgcagcac
    65521 ggatttcttc atattcctgg gcattcatgg actgcagggc attcagtgtc agttcggtat
    65581 tcatcatcgt atttctccct ctgccgcccc gtctccggcg gggcggtgtg gttgttcatc
    65641 aggcggcatc agccaggtta tcggcgcgtt cagtggcatc cgtctgtgga cgtggtgaac
    65701 acatccagcc aggcacccag cggttgtctt tcatatggaa ctctgcatgt tcagcggcat
    65761 cgcctttctt catcttctct gcgtcccgtg cggcaccgga cagtcctgca tcattcaggg
    65821 ctgcgataat ctgcggcttt ttcaggtgtc cgaagaagtt tgctttcgtc ggctgccacc
    65881 agtcgcgcat gtgaaaaccg atggcgctct ccagtgagtc aagcggactg cgtgacgtgc
    65941 gaccacactc gcgggtctgg acaccgttaa ggctgcatgc ggtgcagaaa ctgagcaggg
    66001 ataacagcac ctcctgactg agtgacagga acgtcgtcat gtcccgtgac catccctccg
    66061 gtaacagggc agcaagacgg gctttttctg ccatcagcgc caggaatgcg gcaccttcct
    66121 tccctgacgg cgcatcgctg gtcagcgaat aatgtttaca ttccaggctg atttgtgctg
    66181 gtttactgta cgctccgctg tcaaacacat tcaggcagag cgtccatgcc agcagtgcca
    66241 gggatttgtc cggttgctgc atgagtgccg cctggactgc cagcgtgcgt tcggaagaca
    66301 ttttcgtcag cagtggcaga ctgatttcct ccactgatgc tttctcctgc acctgttccg
    66361 tgcggtcagc gtcgtcagcc gcgtcatcct cgctgcgcaa ctgcacacca cgctggacac
    66421 acacattacc gtaacgccag gacaccacca caccgcttcc ggtacgcatc tccggcgtcc
    66481 acgctctgac cttcgccatg cagcgcatca gcttcatttc tgcttccagc aggtcggatt
    66541 cctcacactg gttttccagc gcgtcgtaac gcgtcatcag ttcgttcagg cgttcttctt
    66601 ccgcctccgt cagcaccgct tccggctccg gcagacagcg gtatgttccg gcatcctcac
    66661 ggcactcacc gacaggctcc atgcgtccgg cgcaccattc ccagccttcg gcttcccgaa
    66721 ggtgttcagc gacagcctgg agtttttcca gcagggcagc atcgagcgcc acgcggtcga
    66781 cataaccgtc cccgtcatcg ctgaacaaat cagtgcgcag ttcgtctggc gagaaagcat
    66841 cagcccccac gaagcggaat ttactgttct ccgccaccgc cacttcactt tccgtaatca
    66901 gattgcggat aacccgcaca tccggttttc cgccccatcc cgactgacag gcggcttcaa
    66961 acacctgtac ctgacgcgcg gtgtcgttct ccagcgccag cgcctgacag tgctcggtgg
    67021 tgatgcggtc ttctgccagc gcatcgagga tgacaggcgc aaggtctgcc agtttcagca
    67081 ttcgctgaac gtggcggggc gaatagccca gcaaatcacc gatttgtgca ggcgttttgc
    67141 cttcctgcgc catcgcacgg aatccggcaa tctgttcggc agggtgcata tcccgacgat
    67201 gaccgttttc agtcattgat gcggcagtcg ccagctcctg cggaatgacc ttcacgcgta
    67261 caggccagtc agccggaagg atgttgcgct ctgccagcat gttgagtgct gccagtcggc
    67321 gaccgcctgc ggcgacaccg taacggtcac ctggcagggc atgaacaacc agattctgca
    67381 gcaggccgac tcccttaatg gaatcagcca gttcactgac ggactccgca gaatacggca
    67441 ccgtgcgcac attcagcggt gatttaatca gtgaatccag cggcacgctc acttcctccg
    67501 tctgcgccag cagggcagac agtgcagttt cctgcgtttt tacagattta cggcttgctt
    67561 tgcgggtggt ttctggttca cacttggtta caggcataat catcccctta taattgctta
    67621 tgtcctgccg gacgttcgcg cgtccggcac ctctcttact taagaatccc gaaatccctg
    67681 atgtactggt tcatccagta cccggcaccg tcctcaaaat tccaggagcg ccagatcaac
    67741 gtgccatcct gacggacaac caggcgaaaa tgcgttccct gatcatcctc gataaagacg
    67801 ttccggtact gtgcggcaac ggcttccgcc tgttcgcggg taaaggagcc gtccggcagt
    67861 ccctgaagta ttctgaaata ttcgctcatc ctcatatctc cggtgatgcc ccgcacaggg
    67921 cggggcggtt gtcacagtca gcccgatcag aacgggatgt cgtctgaaaa cccgtaatcc
    67981 tccccctccg gcggttgcgg ctgaggctct ggctgcgcgg ccttacgtcc acggcctttc
    68041 gttttcgcgc cacctttttt cgtcgcgtca gcactctgtg gctgcccatt ctgctgaggc
    68101 ttcggttgcg cctgagcgtt ctgctgtggt gcacgtccca gcatctgcac ggtgcccgtg
    68161 gtcttaacaa gaatttcagt gacgtaacgg gtgataccgt tatcttccca gctacgggtg
    68221 cgaagctgac cttcgatgta gacctgcgca cccttgcgca gatattcacc tgccacttcc
    68281 gcgagcttgc cgaacaacac cacgcgatgc cattctgtct gctcccgcat ctcccccgtc
    68341 tgtttgtcac gccagctttc tgacgtggcc acctgcaggt ttgccactgc gcccccgttt
    68401 gggatgtaac ggacttccgg atcttttccc agacgcccga cgaggatgac cttgttgata
    68461 ccacgtgcac tcataagaac ctcctgactg aaagatgtga gataaaccgg ataccggcga
    68521 cgggacgttc atgtccccct tcagaggagc gaaagctcct cctgccgtcc acttcacagg
    68581 gttttctccc cgcagtggac ctttccgatg ggggcccgtt taccgcctca cgggacagct
    68641 gtccgggggc ggacgcaaaa ttttttgccg ggttcatggc taaaaatttt gtggccggtg
    68701 catctcaccc tggacagatg gaacgggagg gggttacgtc tgccccccgg aaagtccaca
    68761 gcgggaaaca ggccgcagag gtggcggcag gagggaagac tgctgcgcca tcaggcgcag
    68821 tttcctcaca caccgtcagg tgtgcaggga gcgcaggtca cggcgcgaca ggggcggcgc
    68881 gaccggaacg gtcgctgcaa gccgggtttt cacgcatcgg accgtagcgg cagcccgaaa
    68941 ccgcgccagc gggttcggcg gagactggcg ggtgcgccag cagctgcctg tcggagtcgg
    69001 gcctgacgac cggatgccgc caggcagccg ggagccgaag tgggcgcaat aaaagacagc
    69061 cagaaacggt gacagtgtca cggaatatca cacctgttcc ctgcatacag ccatacacta
    69121 ccttctgacc ggaaagccgg gccggcagcg ccgcaggcgc tgcaccccgc atttccggca
    69181 ggcgccagcc tgccaggggt tttcgcgcgg gctgacaggc ggcctccacc gcctgccctg
    69241 tatcacctgt ttctgctgcc ctgtaagatg tagtgaccgc ccaggaaaac cgcctgctga
    69301 ttcgccacaa aatcgcagat ttcatcaaca atctgcgcgg tggtgagcgg tttctgcccc
    69361 atttcacgac gccgcgcatt aatcacggcc ctgtaattac gcagaatttc gcgggtttct
    69421 gtgctgatat tcatgacatc gtctccccgt ccagcgcctg caacacaacg tccattgctt
    69481 cgagttcctc cctgacgaat gcgtcaccgt cgcccccgtt ctcactcccg acggcataca
    69541 ggcgcttcat ttcactacct gccagcgcca tcactttttc cagcatcacc gccgggactg
    69601 tcacatgacg gctggcgcgc ttttcttcca tcagttcatt gacaatatcg gaaacaactc
    69661 tgtcacatgc gccatataca aaagcatcgt ccagccgctg catgacggtt tccagctcat
    69721 cgtctgacag gctccagtcc cgggctatgt gacgcacatc cccacgggtc agcactacgg
    69781 caaaaacgca ttcactgtcc ggcacgtctg tatccagcac ttcgcggata atttctttaa
    69841 cagtacagta catgttctcg tctcctttac aggggcggca ttgccgcccc gtccgttaat
    69901 cagtccagcg ccgcgtaaat cgcgttgcat tcagggtgaa attcgatgtg gctccacaac
    69961 tgcgcatcac gcatcctgta caggtgtgtc aaccctgcat ccccgctgtc gtggtacagc
    70021 cacaactggc ggttaatcac aagggaggta aggatgatgc ctgcggcatc agcactgacc
    70081 gtgcggtcaa accagttttc accgttcacc atgtggaaac ggtcgccatc cggcatcatg
    70141 taaccgccac cgtccggcag gcggatgaag tgccagaatc cgccttcata ttccggcatc
    70201 atttttttcg ccagcgcgca cacagacgct tcgacataca gaaaatcctg gccaaacagg
    70261 taaggcagaa aaaacagacg gtcgcactct tcaacattca gagtgacagg tttcgcatac
    70321 tgcatgggat ttcctccgtt gttcacatat tccgcgacac cgggcggtgc cgtgcctcca
    70381 tctttgccgg gcctgcaggg ttgcaagggc gcaagggaag ccgcagcctg acgcacacgg
    70441 ctggcgggag ttggagggtg agaccgcgaa atctgagcgg gtgaacgacc ggaaaatcac
    70501 ggcagacggg aaggagggac cgggtgaccg cagcggttca ccttaccttg caacccgccc
    70561 gcagaagccc ggctacgatg gaggcacggc acagcccgca cacgacgctg aggccacata
    70621 actgaaaacc cacaaggcca tgccccgcat ggccgggtaa agcggagcct gcggcgacat
    70681 ccttttgctc tttccgggga ccggctggaa aaggcggaga tttttccccg ccagcgtcat
    70741 catttccgga ttttcgccag tgcatccgcc atgctgcgga tttcagtccg ggcttccagg
    70801 accacctccc gcagaatatc ccggtcaaac cagcgacggg acggttcatc aggtatacgc
    70861 agcacatcgc aggccagccc actggacaga atatgctcca cacactcact gaccagatac
    70921 atttccaccg acgaaaaacg agcgccccgg ttcaggatgg catcgagcac cacgggcagg
    70981 ttttcgtcgt cttcatcgag ccagcagggg tgatcgcttt ccttcacaac caggcgaaag
    71041 cggcgcgtct ggcgggtcag caacgtctga ccggttgtgg tcagcgccgc cctgatacgc
    71101 cgcgcatctt ctgccacccg gcaactgaca ctgcgtaccg catcgtgcac cgcatcaaga
    71161 actgccctga tttcgtcttc ggtcagccat tctccggcat acgggaaaga cacctgcgtt
    71221 ccgtctgccg gaagtaccgg cagcgactgc cactgactgc cggacagaac aagttccgcc
    71281 ccggttccgg tcagtttctg aatggcagag gccaggagcg gcggcagtac gccgccgtga
    71341 cgattgataa gtaacgtcgc cagccggggc gcatccagca ggttacgggc gtggcgacaa
    71401 atcagtgcat tcagcgtttc gttcatgatt catggtctcc ctgaatcgcc gcccgcaggc
    71461 ggcgataaca tcagtcgttt tcgcgtgtgt tgtttttcag cagggcgcgg gcctgattaa
    71521 ccaccgcctg tcggcgggtg acgcattccg ggacggcgag gccgttatca cgggcggccc
    71581 actcacgttt ccacagtgcc tgttcggcgg tctgaatcag ggacgccagc agttcagcag
    71641 ggacggtcac ctggcggttt tctctcacgt tattgatgat ctccatcgcc gcaccggaag
    71701 aaataccgga ttcagtccgc tggtcttccg ggatgtcctc caggcgcgcc aggacagtcc
    71761 tgatttcatg atcggacagg gaaatatcca ttccgtcagc gagggcctga atttcttccg
    71821 gggaccagat aaccagcatc agcggcgtat ctcccggata ctttgctgcc agcgcccggc
    71881 agagcgtttc gcatgttccg tacattctcg tctccttctc aatctttgaa gcaggaatcc
    71941 agttccagaa tttcggtata cgcttccatg aacaggcgat tggcgcgata cagcgtctgg
    72001 cgtgtcaggc gttcaacaag cacatcggaa tcaaagcgac agcgatgctc catatcgttc
    72061 agcctggccc gaaggtcaca catggtgttg gccacctccg ccacgtcggc gcgaagtttt
    72121 tcgtgtccgg catagttcat ttacgcagcc tccggttcaa accagttatc attcgcggcc
    72181 ccctgctgca tggcccgttg cacggcggtc agtcgttgct gtccggcacg gtgatactgt
    72241 tcaagcagct cgataccgat ataccggcgt ccggactgaa gagcggccac gcaggttgat
    72301 ccgctgcctg caaacgggtc cagcacaatc gcgttcggat gtgtgaagct ctcaatcagt
    72361 ggttgcaggc tggtaacggg cttttccgtc gggtgatggc gattgcccga atatttccag
    72421 cccagcacgt ccggcagcgg gttttgtggc agacgtggac ggccttttgc caggatgtag
    72481 gcacattcgt ggcgatagcc cacatatgcg gccttcgatg tgtagttttt ggtgaagacc
    72541 aggtgaccaa caacgctgaa tcccgcattt ttccaggcag acataaaacg atcgacgcga
    72601 ttccagccat agaagctcac cattaacgcg tcttttttga gtacgcggta catctcatta
    72661 caggccggtt gcagccattc atcggtttta tcgccggcaa tggtacgccc ctgacggtca
    72721 cgaaaaccga cgaggtacgg cgggtcggtg aggatgaaat caatggcgtt atcagggata
    72781 cgggccataa catcgatgca gttaccgagg acaaaacggg acataccaaa ctccttctct
    72841 tacagagggc accgggaggg cacccctgtg gtgcccgttc cccgctgcct gtgggctgtt
    72901 caccgcgtcc ggtaacgggt caggccgacc gtggaatacc gcagccgtcg tctttttgac
    72961 ggcaaggcta tgccgcgaaa gcggcttgaa gggcgttcat cagaaaaggg cgacaggctg
    73021 gcgtacactg tcaccggtgc cagccgggag cggtaaaagc gcccgtcccg tgcccaccgg
    73081 acgcgggaca ctgaccacag gcagcgttta tgccgcgccg cgtcagccgg cgcagtaaaa
    73141 agaggagcac ggcgacatcc tttaccggaa aagaaacggg cggttgcccg cccgtctccg
    73201 tcatacttca tccgccataa tgcgccacat tgtcgactat ccacgcaggt ccggtgactt
    73261 ccggcagctc atcgtcatct gtcggggaag accattccag ttccccccac agcacatcaa
    73321 caagctctcc gcgctcatac aactgccagc cacagaaatc acagccctgt tcggcgtacc
    73381 agtgctccag cgtgcagccg aaaaggcggc ttaattctgc aacaacgtcg ctttccggct
    73441 gacaccacgg cgtgtcgaaa tctacctgaa tgaagttttc cccctgactg ctgatattca
    73501 ggtcataccc acaaggccac ttcacgccat accgttctgt ataccagtga tatgcagaag
    73561 gaacaccgtt cagaacaccg ccgttaaagc cattcacttc aatatccagg cgggtcggca
    73621 aaaccgtcag caggtcaaac ggcatggttt tttccggcag tacgttgtca cacagacggt
    73681 tccaccacac cgatacatcc tcgttactcc agaagccaca ccagtcacct ctttttgcgg
    73741 tgaaaagtgc ggtgatggta tccctgacat cgtccggtaa tccttcccag cgacgctgtc
    73801 cagtaccgct ctgcagccag agttcatgca gcatcaggca gttttgctca tccagcagta
    73861 caccgttctg caggtgtgtc agccagcggg tgaacgcgat attttccggt gatacagcac
    73921 cacgtccggc agccgtcact ccggggcact gctcagagcg gatattttca gcgatctgca
    73981 gaagtccggc acttccggcc agaaacagct gaataccttc atttgtggcg cggcgataaa
    74041 gcggtgtaac tgcaccgctg gccagtcgtt taatctcagc gatctgtgcc ggttcaccag
    74101 aaaaatacat acgattcgag caccagtttg gcatgggaat tctcctctca catgcggcct
    74161 ccatcaggcc gtgccgcacc tgttaccggg cgaccgcata cgaatgcagg tgcggccccg
    74221 aagccgcagc gcgtggagga ggaacgacga aacgtgaccg ggtgaggagg ccggcgacag
    74281 cgacacactt gcatcggatg cagcccggtt aacgtgccgg cacggcctgg gtaaccaggt
    74341 attttgtcca cagaaccgtg cacaaaatgc tgtggataag ctggacacag cagcccacag
    74401 caggcataca gccacacact cagagaattg agcgtttttt agtcacaaca atgacatttg
    74461 actgtgtgcg gattcagtta ttagaatact gtataaacat acagcataaa gaaaagggag
    74521 atgagaacat gatccgcatt gaaattcttt tcgaccgcca gagcacaaaa aacctcaaat
    74581 caggcacact tcaggcgcta cagaatgaaa tcgaacaacg tctgaaacca cactacccgg
    74641 aaatctggct gcgtatagac cagggcagcg ccccgtctgt atctgtcaca ggagcccgca
    74701 acgacaagga taaagaacgt atcctgtctc tgctggaaga aatctggcag gacgacagct
    74761 ggctgcctgc agcatgagta ccgtttatca ccgtccggct gccccttcag gcgatgacag
    74821 ttacgtgcga ccgttgtttg ccgatcgttg ccaggccggt tttccttcgc ctgccactga
    74881 ttatgctgag caggaactgg atctgaacag ctattgcatc agcagacctg cagccacctt
    74941 ctttctgcgc gccagcggtg aatcgatgaa ccaggctggc gtgcagaatg gtgatctgct
    75001 ggtagtggac agggccgaaa aaccacaaca cggggacatc gttatcgctg agatcgacgg
    75061 tgagttcacc gtcaaacgac tgctgttgcg cccacgcccg gcactggagc cggtttcaga
    75121 cagcccggag ttccgcacac tgtatccgga aaacatctgt atttttggtg ttgtcactca
    75181 cgtgatacac aggacgcggg agttacgctg atgtttgcac tggctgatat caacagtttc
    75241 tacgcctcat gtgaaaaagt tttccgcccg gaccttcgca acgaaccggt catcgtactc
    75301 agcaataacg atggctgtgt gatcgcgcgc agcccggagg caaaagccct tggcatcaga
    75361 atggggcagc cctggtttca ggtgagacaa atgcgcctgg agaagaaaat acatgtattt
    75421 tccagcaatt atgcgctgta ccacagcatg agccaacggg ttatggctgt tctggagtcg
    75481 ctttctcccg cagttgagcc ctactcaatt gatgaaatgt tcattgattt gcgggggata
    75541 aatcattgca tctctccgga gatttttggt catcagctca gggaacaggt aaagagctgg
    75601 acaggactca ccatgggggt gggcattgcg cctacaaaaa cgctggctaa aagtgcccag
    75661 tgggcaacaa agcaatggcc acagttttcc ggtgtggtcg cgctgacggc agaaaaccgt
    75721 aatcggatct tgaagctact ggggttgcag ccagttggtg aggtctgggg agtaggacgc
    75781 agactgacgg aaaagctgaa tgcgctgggt attaacacag cactgcagct ggcgcaggct
    75841 aacacggcat tcatccggaa aaacttcagc gtcattcttg agcgtacggt acgcgaactc
    75901 aacggcgagt cctgcatatc tctggaagaa gcaccgccgg caaaacagca gattgtctgt
    75961 agtcgcagtt ttggtgaacg aatcacagac aaagatgcca tgcaccaggc tgttgttcag
    76021 tatgcagagc gggccgcaga gaaactacgt ggggaacgtc agtattgccg gcaggtgacg
    76081 acatttgtac ggacatcccc ctttgcagta aaagaaccct gttacagcaa tgccgctgtg
    76141 gaaaagcttt cattgcccac acaggacagc cgggacatta ttgccgccgc atgcagggcc
    76201 ttaaaccatg tctggcgtga agggtaccgc tatatgaagg caggagtcat gctggctgat
    76261 ttcacaccat cgggtatagc acagccggga ttatttgatg aaatccagcc ccgtaaaaac
    76321 agtgaaaagt taatgaaaac actcgatgaa ctgaaccagt cgggaaaagg gaaagtgtgg
    76381 tttgcggggc gaggaaccgc ccctgagtgg caaatgaaac gggaaatgct ttctcctgcg
    76441 tataccacca gatggacaga tttgccagta gcccagttat gaatatgtaa tccacccata
    76501 atattgttat cttgttatct tgttatcttg ttatcttgtt atcttgttat cttgttatct
    76561 taattaatct ataaatcaaa tacttaacaa cttattaagt tgttaatata acaaaaataa
    76621 ttgcatgatt cgcgttcaga tggcaatatt aagttgttaa cttaacaact taatatgggg
    76681 ctataatgat aatagtatta ggtagtcaaa agggtggggt aggtaaaagt acactggcgg
    76741 tatctatagc tgcgtatctg atgtcactag gcaatagagt gattattgta gacgcagatg
    76801 accagaaatc agtattaacc tggtacaaca atcgccctga agatctccct catattccgg
    76861 ttacaggcgc gacaggtaac atcaaagcca tgttaaagga acacgaaaaa tcctatgatt
    76921 ttgttattgc agactgtgct ggccgtgata gcgcagaaat gcgtagtgga ttgatggccg
    76981 cagatgtatt catttctcct ttgcgcccat ctcagatgga cctggatgtt gtgcctcata
    77041 catgctccgt ttttacagca gcaaaagatt ttaatgagga tgttcgggga tatctcgttt
    77101 taaacatgac gcccacaaat atgtttgtta atgaagccaa tgaggcagct gaagtactta
    77161 aggattaccc tgaaatgcat ctggcaaact cccgggtatg tgatcgtaaa gcacatcgag
    77221 atgcttgggc cgaatcaatg acaatttttg aaacgcaaaa tgataaagcc cagcaagaaa
    77281 tcgaagcgtt agtgaaagag gttattctgt gagaaataga gcaaacactt cacctcgtga
    77341 cgaggcagca tttattaacg gtggcacggc tggccttgat aataccaaaa agtcatcaga
    77401 taaatcatcg gttgtaagca aagcaaaacc agtgagtatt tctttttttg aagaaaacct
    77461 aaaagacatt gatgccctta taacggctga gatggtttca ggtaaaggca gagtgaatcg
    77521 ttctgatgtt gtcagggcgg cggtagaagc acttaaaaca ctatcaaaaa cagaggtatc
    77581 acgattgata gcagaagtaa aacaaagata acaccttgtt atcttgttat cttgttatct
    77641 tgttatcttg ttatcttcaa tgtattttag gatgacctat gttgaataga acctaaaaca
    77701 aaacaaatat taataggggt ccgcttggaa ttcggaaatt ttcccacgct aagagaattg
    77761 agttgaacga gtggttacat ccaggccagc taactcactg aacgtaaagg ctgtacgaaa
    77821 tgttccggcc gtccgataac cgtttggctt acttccgtac attcccttgc gtagatgtac
    77881 agaattgtac gatttcggac accaaaaccg ctgttgtccc gccattctgg cgattcctga
    77941 aagtcctttg actaaaaaat agtcttaacg tggtttattt tccgaattcc aagcggaccc
    78001 cagtaagtaa aatatacaaa gaacacccca tgaaacaaag gcagagatga accacggcag
    78061 cctggcggct gcaaatcggg gtgtggcaga atatgagctt tcagagtaac catccagaat
    78121 gagttcccat gtcacacaac ctggagcacc agaaagtcca caccagaatg gtgaaagagg
    78181 ttctgaaagc cgtcgccaga gccaataatc acccttatca gtccgtattt gcagacttta
    78241 tcaccggtca tccctcctgc acggtctgtt tctgggaaac cttccacaaa atgtacccgg
    78301 attcaccgta cgaatacgtc acgttctgcc atacctgccg tcgttttgat ttatacgaaa
    78361 cagaagcgga gatgaaggcg gatgacccga agtggtggtg attttcagcc ccgaccgctg
    78421 aaacgcctgt tcacagccaa ccagtgctgg acgtccttcc tggatgcggg cggtctgcgc
    78481 gatatcgagg ttgaggctgt cacaaaaatg ctggcctgcg gcacgcggat actgggagtg
    78541 aaggaataca actgcgataa acctgagtgc ccccacgtca gatacgtcac taactcatgc
    78601 ggcagccgtg cctgcccgtc ctgcggaaaa aaggccacag acctgtggat agcgacacag
    78661 ctgaatcgtc ttcctgactg cgactgggta catctggtct tcaccctgcc ggacacgctg
    78721 tggccggtgt tcgaaagcaa ccggtggctg ctgaatgacg tgtgccgtct ggcggtggag
    78781 aatctgctgt atgccgcccg gaaacggggg caggaacccg gtatcttctg cgccatccac
    78841 acgtatggcc gtcgtctcaa ctggcatccg catgtacatg tgtctgtaac ctgtggatgt
    78901 ctgaataagc atggtcagtg gaaaaagctg agcttcctga aagacgcgat gcgttcgcgg
    78961 tggatgtgga atatgcggca acggcttctg aaagcgtggt cagaggggct ggcgatgccg
    79021 gagtcgttgt cacatatcac gacggaatca cagtggagaa gcctggtgct gaaagccgga
    79081 ggaaaatact ggcatgtgta catgtcgaaa aaaacggccg gcgggaggaa tacggcgcgc
    79141 tacctgggtc gttatctgaa gaagccgccg atagcggcct cccggctggc acattacaac
    79201 ggaggggcga gcctgagctt ccgttacctg gaccacaaaa cgggagaaac ggcgacggaa
    79261 acgctgacac agcgtgagct ggtcgcgagg ctgaaacagc acatcccgga gaagtttttt
    79321 aagatggtga ggtacttcgg gttccttgcc aaccgggtgt gtggagagaa gctgccgcag
    79381 gtgtaccgtg ctctggggat ggataaaccg gaaccagtgg cgaaagtgtg ctatgtacaa
    79441 atggtgaaac agttcctgag tcgtgacccg ttcgaatgcg tgctgtgtgg cggccggatg
    79501 gtataccgcc gggccattgc ggggctgaat gtggaggggc tgaagaaaaa cgcgcgggat
    79561 atcagtctgc tgaggtatat gccggcctga cacaggctaa gtgtgcccgg gaatcggtgc
    79621 aagtataaaa aacagcctgt tgcggtataa gaatcgacca tgcagagaga gaaaacaccg
    79681 gagtggtggg gaaaaacaga aaggctcacg tggcataagg cgcggacagg ggtatcgctt
    79741 agtttttcaa tttcctatac gtgaactgtg cgccccgatt tcttcttctg atatcctttc
    79801 aaatgaattg tttttattta tatcatgaca acataaaagt gagagaatat atcaaccatg
    79861 tttctgtcag ggttcagtag aatatcaatt cgactgtcac gccataactg ttactacctc
    79921 gagtataaat attcagagaa cggacttatt agccatgaaa aatcacctga tggaaaattt
    79981 actaatttat gaattgtgat attattaaag agtaaaacca gccagccgag gtatttatgg
    80041 aaaaaaatca ggaagaaagg aaaactcccg tcattgtggt gaaaaaacgt cgtacttttt
    80101 cgctaccgtc cttatccgaa aaaacagata ttatcgcacc tgtatttact gaacagacgg
    80161 cagaatcagc ccccgccgga ataaattcat ctgccgtcga aacacatatc cccgaagcgc
    80221 cagccaggaa gaagaaaaag aaaagacacc gttttccacg tccctcccac tggacccgtg
    80281 agtatactca cgagtgcgtg gaaaaaataa aggccctgtt tcctcatctt cgtgctgaag
    80341 gtggtggatt tattcccctg aaaattggca taaacaatga tatttcggca tttctggcag
    80401 aaagcccgga tacagaactg tccatggacg aatggttctg tgcagtgtcc tgcatcacct
    80461 cccgccgcgt atatctgcag cggacctctg tcgccggcgt ccctcgttat gggctggatg
    80521 gtcatcccga tgggcaggtc tcagaaactg aagcacagag tgctgttcaa agactggcga
    80581 taatagaaca acggtggctc aggatgaagg aacggcagac tgagcaataa acatttcaga
    80641 taacatcatg acattgtgta tcagagtctg cttacagact catggatgcc accaaaggct
    80701 catcagatgt aaggcagaag cgacgtaact gccttttgaa aggaaaaaag cgaccgcaag
    80761 gatgcaatgg atatctgccg catactgaaa taaagtaaca aaaaaatcac cacaatcacg
    80821 ggggcttcct tcctgcactc aggccatacc ccccgcacgt ataaatttct caatcaacaa
    80881 tacaataaaa ctgtgaataa tatcatcatg attaacgcat aattacccgt gattctttcc
    80941 tggtaacgta agtacagtta tccggaacct cctccataat aaaagacatg gctccaattt
    81001 ttacattact gcctattttt acgttaagcc cgataataca cgtatgggca ccgatatcga
    81061 cattatcacc aataacgata taattctctc tgctgtcaga ttccttttcg ccaatggtcg
    81121 tattctgccg tataatcaca ttctcaccga tttttacatt tccgtgaatg acgacgcctg
    81181 aatggtgggg cagcacgagg cctttaccaa tctgcgcccc cagaccaata tcacatccat
    81241 actttgccat aagcgtattt tttatcttga tcgcacattt tcgttgtttt ttattcccca
    81301 atatatacat ttcattagcc aggcgccacc acagaagaaa acctctctgc ctctgcatac
    81361 cgttcctgat aagccggtga aaaacaaact tatcccgcat gataacttct gctttccagt
    81421 attccagaag atgacgtttt tttcctgaaa ataaaaacgt gccaatatgg aaataagaaa
    81481 tcggattttt tatcaacata cgcaaatttt tagaaaacaa tgaatacaga tgtattttat
    81541 atacacagat aaaaccg
//
LOCUS       DAEEKQ010000002        80167 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.002, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000002 DAEEKQ010000000
VERSION     DAEEKQ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 80167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 80167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..80167
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.002"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            75..359
                     /gene="pduA"
                     /locus_tag="KLK57_000092"
     CDS             75..359
                     /gene="pduA"
                     /locus_tag="KLK57_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduA"
                     /protein_id="HBE0890585.1"
                     /translation="MQQEALGMVETKGLTAAIEAADAMVKSANVLLVGYEKIGSGLVT
                     VIVRGDVGAVKAATDAGAAAAHNVGEVKAVHVIPRPHTDVEKILPKGINP"
     gene            356..1168
                     /gene="pduB"
                     /locus_tag="KLK57_000093"
     CDS             356..1168
                     /gene="pduB"
                     /locus_tag="KLK57_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduB"
                     /protein_id="HBE0890586.1"
                     /translation="MSSNELVEQIMAQVIARVATPEHEVLPKTTHQIRETAMAEKSCT
                     LTEFVGTAIGDTVGLVIANVDSALLDAMKLEKRYRSIGILGARIGAGPHIMAADEAVK
                     ATNTEVISIELPRDTKGGAGHGSLILLGGNDVSDVKRGIEVALKELDRTFGDVYANEA
                     GHLELQYTARASYALEKAFGAPLGRACGVIVGAPAAVGVLMADTALKSANVDVVAYSS
                     PAQDTSYSNEVILIISGDSGAVRQAVISAREIGKTVLSTLGDTPKNDHPSYI"
     gene            1187..2851
                     /gene="pduC"
                     /locus_tag="KLK57_000094"
     CDS             1187..2851
                     /gene="pduC"
                     /locus_tag="KLK57_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase large subunit PduC"
                     /protein_id="HBE0890587.1"
                     /translation="MRSKRFEALAKRPVNQDGFVKEWIEEGFIAMESPNDPKPSIKIV
                     NGVVTELDGKPASQFDLIDHFIARYGINLARAEEVIAMDSVKLANMLCDPNVKRSDIV
                     PLTTAMTPAKIVEVVSQMNVVEMMMAMQKMRARRTPSQQAHVTNVKDNPVQIAADAAE
                     GAWRGFDEQETTVAVARYAPFNAIALLVGSQVGRPGVLTQCSLEEATELKLGMLGHTC
                     YAETISVYGTEPVFTNGDDTPWSKGFLASSYASRGLKMRFTSGSGSEVQMGYAEGKSM
                     LYLEARCIYITKAAGVQGLQNGSVSCIGVPSAVPSGIRAVLAENLICSSLDLECASSN
                     DQTFTHSDMRRTARFLMQFLPGTDFISSGFSAVPNYDNMFAGSNEDAEDFDDYNVIQR
                     DLKVDGGLRPVREEDVIAIRNKAARALQAVFAGMGLPPITDEEVEAATYAHGSKDMPE
                     RNIVEDIKFAQEIINKNRNSLEVVKALAQGSFTDVAQDMLNMQKAKLTGDYLHTSAII
                     VDDGQVLSAVNDVNDYAGPATGYRLQGERWEEIKNIPGALDPNEID"
     gene            2861..3529
                     /locus_tag="KLK57_000095"
     CDS             2861..3529
                     /locus_tag="KLK57_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol/glycerol family dehydratase medium
                     subunit"
                     /protein_id="HBE0890588.1"
                     /translation="MEINEKLLRQIIEDVLAEMQPSDKSVSFRAPVSATVPSAPDTGN
                     FLTEIGEAQQGTQQDEVIIAVGPAFGLAQTVNIIGIPHKNILREVIAGIEEEGIKARV
                     IRCFKSSDVAFVAVEGDRLSGSGIAIGIQSKGTTVIHQQGLPPLSNLELFPQAPLLTL
                     DTYRQIGKNAARYAKRESPQPVPTLNDQMARPKYQAKSAILHIKETKYVVTGKKPQEL
                     RVTF"
     gene            3544..4062
                     /gene="pduE"
                     /locus_tag="KLK57_000096"
     CDS             3544..4062
                     /gene="pduE"
                     /locus_tag="KLK57_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol dehydratase small subunit PduE"
                     /protein_id="HBE0890589.1"
                     /translation="MNTDAIESMVRDVLNRMNSLQDAAPVSAVPNASILSAKVTDYPL
                     ANKHPEWVKTATNKTLDDFTLENVLSDNVTALDMRITPETLRIQAAIARDAGRDRLAM
                     NFERAAELTSVPDDRILEIYNALRPYRSTKQELIAIADDLEQRYQAKICAAFVREAAE
                     LYVERKKLKGDD"
     gene            4072..5904
                     /locus_tag="KLK57_000097"
     CDS             4072..5904
                     /locus_tag="KLK57_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diol dehydratase reactivase subunit alpha"
                     /protein_id="HBE0890590.1"
                     /translation="MRYIAGIDIGNSSTEVALATLSATGELSFVSSALEETTGIKGTL
                     RNVHGIQEALAQATKKVGINVSDISLIRINEATPVIGDVAMETITETIITESTMIGHN
                     PKTPGGVGLGVGFTITPQELLTRPADTPYILVVSSAFDFADIATMINASVRAGYQLTG
                     VILQRDDGVLVNNRLEIPLPIVDEVLYIDRIPLGMLAAIEVAVPGKVIETLSNPYGIA
                     TVFALNAEETKNIVPVARALIGNRSAVVVKTPSGDVKARSIPAGNIELLSAGRTTRVD
                     VAAGADAIMKAVGECPKLENVTGEPGTNIGGMLEHVRQTMAELTNKPSNEIFIQDLLA
                     IDTSVPVSVTGGLAGEFSLEQAVGIASMVKSDRLQMAMIASEIKQKLHVDVQVGGAEA
                     EAAIQGALTTPGTTRPLAILDLGAGSTDASIINQSGEIVATHLAGAGDMVTMIIAREL
                     GLNDRYLAEEIKKYPLAKVESLFHLRHEDGSVQFFPTPLSPHVFARVCVVKPDELVPI
                     PGDLTLEKVRAVRRSAKERVFVTNALRALRQVSPAGNIRDIPFVVLVGGSSLDFEVPQ
                     LVTDALAHYRLVAGRGNIRGSEGPRNAVATGLLIAWHKESIHGK"
     gene            5894..6244
                     /locus_tag="KLK57_000098"
     CDS             5894..6244
                     /locus_tag="KLK57_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydratase reactivase beta/small
                     subunit family protein"
                     /protein_id="HBE0890591.1"
                     /translation="MESKERAPAIVVMEIGDCITTWDEVLLGIEEEGIPFRIQHIPSG
                     EVIDSAWLAARQSPLLVGIACDREELIVHYKNLPASAPLFTLTYQQDNHTRRSIGTNA
                     ARLVKGIPFREFHS"
     gene            6264..6539
                     /gene="pduJ"
                     /locus_tag="KLK57_000099"
     CDS             6264..6539
                     /gene="pduJ"
                     /locus_tag="KLK57_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduJ"
                     /protein_id="HBE0890592.1"
                     /translation="MNNALGLVETKGLVGAIEAADAMVKSANVQLVGYEKIGSGLITV
                     MVRGDVGAVKAAVDAGSAAASAVGEVKSCHVIPRPHSDVEAILPKSA"
     gene            6563..6985
                     /locus_tag="KLK57_000100"
     CDS             6563..6985
                     /locus_tag="KLK57_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMC domain-containing protein"
                     /protein_id="HBE0890593.1"
                     /translation="MKQSLGLLEASGLALAISCADAMAKAASITLVGLEKTNGSGWTL
                     IKITGDVASVNAAIATGASFAGQRNGLVAHKVIARPGDGILPHTTEALTVTVETSKTP
                     ATTVTSEIISCNLCLDPACQRQKGEPRSLCLYANKRGE"
     gene            6988..7620
                     /locus_tag="KLK57_000101"
     CDS             6988..7620
                     /locus_tag="KLK57_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate propanoyltransferase"
                     /protein_id="HBE0890594.1"
                     /translation="MDKQTLQETVSQILDEMRERPIPLGVSSRHIHLSSADYLRLFPE
                     QPISEKKPLMQPGQYAAEQVVTLEGPKGKLHNVRLLGPLRNASQVEISRTDARTLGIT
                     APLRMSGDLSGTPGIRLISPFAELEITSGVIVAQRHIHMSPLDALIYGVTHGDKVAVA
                     IEGDERRLIFENVAVRVSPDMRLEMHIDTDEANAAGADSPDVFATLVSVP"
     gene            7617..8108
                     /gene="pduM"
                     /locus_tag="KLK57_000102"
     CDS             7617..8108
                     /gene="pduM"
                     /locus_tag="KLK57_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcompartment protein PduM"
                     /protein_id="HBE0890595.1"
                     /translation="MNNMLMQRIVDEVVFRLKQRAGKTLVLTVFQLRDASVQESVHQY
                     ASLQIRYVDLPLLRQLAENETSDRASIQIHEALAWGMHIQLSLQRHFLNAIELKTLAR
                     LPLSWCDEQGQPIYLHHNRLLSYADIAQLSSGILVLQRKCCVTALAREAAITRNIQLI
                     RQE"
     gene            8112..8387
                     /gene="pduN"
                     /locus_tag="KLK57_000103"
     CDS             8112..8387
                     /gene="pduN"
                     /locus_tag="KLK57_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduN"
                     /protein_id="HBE0890596.1"
                     /translation="MHLARVTGAVVSTQKSPSLIGKKLLLVRRVSADGELPLTPLTGD
                     EVAVDSVGAGVGELVLLSSGSSARHVFSGPNEAIDLAVVGIVDTLSR"
     gene            8399..9406
                     /gene="pduO"
                     /locus_tag="KLK57_000104"
     CDS             8399..9406
                     /gene="pduO"
                     /locus_tag="KLK57_000104"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-domain cob(I)yrinic acid a,c-diamide
                     adenosyltransferase PduO"
                     /protein_id="HBE0890597.1"
                     /translation="MAIYTRTGDSGSTSLFTGQRVSKTHLRVETYGTLDELNATLSLC
                     YCATAIESHRILLEAIQQQIFWFSAELASESEQPSAQQRYIGTEEIAALENAIDSAMN
                     AVPPVHSFILPGRCEAASRMHFARTVARRAERRLVELTTETTVRNVLLHYINRLSDCL
                     YALARVEDNVAHQNLMIQEITKRYHEANHTPALKERTMPLTFQDLHQLIRSAAMRADE
                     LHIPVVISIVDANGTESVTWRMPDALLVSSELAPKKAWTAVAMKTATHKLADTVQPDA
                     PLYGLESHMQGKVVTFGGGFPLWRDGKLLGGLGISGGSVEQDMDIAQSAMAAINVGVN
                     Q"
     gene            9403..10788
                     /gene="pduP"
                     /locus_tag="KLK57_000105"
                     /pseudo
     CDS             9403..10788
                     /gene="pduP"
                     /locus_tag="KLK57_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460996.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating propionaldehyde dehydrogenase
                     PduP"
     gene            10799..11911
                     /locus_tag="KLK57_000106"
     CDS             10799..11911
                     /locus_tag="KLK57_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001091448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HBE0890598.1"
                     /translation="MNTFSLQTRLYSGPGSLKVLSRFSHRHIWIICDAFLARSPLIDT
                     LHQALPDSNRLSIFSEITPAPTIQTVVKGIAQMQTLRPDVVIGFGGGSALDAAKAIVW
                     FGRQCGIEIETCVAIPTTSGIGSEVTSACVISDPEKGIKYPLFDNALYPDIAILDPSL
                     VVSVPPAITANTGMDVLTHALEAYVSPRASDFTDALVEKAVQIVFQYLPTAVKKGDCL
                     ATRGKMHNASTLAGMAFSQAGLGLNHAIAHQLGGQFHLPHGLANALLLTAVIRFNAGD
                     PRTAKRYARLAKTCHLCPDNANDTASLNALIQHIEQLNTTCTLPTLANALKEKKAEWS
                     IRIPDMVQAALADATLRTNPRAADASAIAELLKGLL"
     gene            11908..13253
                     /locus_tag="KLK57_000107"
                     /pseudo
     CDS             11908..13253
                     /locus_tag="KLK57_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585845.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SLBB domain-containing protein"
     gene            13256..13810
                     /gene="pduT"
                     /locus_tag="KLK57_000108"
     CDS             13256..13810
                     /gene="pduT"
                     /locus_tag="KLK57_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduT"
                     /protein_id="HBE0890599.1"
                     /translation="MSKAVGILELSSIAKGMETGDAMLKSANVELLVSKTLCPGKFLL
                     MLGGDVGAVQQAINSGASLAGEMLVDSLVLPNIHPSLLSAISGLNHVEQHRAVGVVET
                     WSVAACIDAADCAVKAANVILVRVHMAFGIGGKCYMVVAGDISDVNNAVSAASVRAGE
                     KGLLVYRSVIARPHEAMWRQMVEG"
     gene            13810..14160
                     /gene="pduU"
                     /locus_tag="KLK57_000109"
     CDS             13810..14160
                     /gene="pduU"
                     /locus_tag="KLK57_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000441103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol utilization microcompartment protein
                     PduU"
                     /protein_id="HBE0890600.1"
                     /translation="MTTQHTTERMIQEYVPGKQVTLAHVIANPGKDLFKKLGLPDTIS
                     AIGILTITPSEASIIACDIATKSGAVEIGFLDRFTGAVVLTGDVSAIEYALSQVTRTL
                     GEMMRFTACPITRT"
     gene            14165..14608
                     /locus_tag="KLK57_000110"
     CDS             14165..14608
                     /locus_tag="KLK57_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutP/PduV family microcompartment system
                     protein"
                     /protein_id="HBE0890601.1"
                     /translation="MQRMMLIGPSQCGKTSLTQCLRGENIHYQKTQAIVWSPTTIDTP
                     GEYLENRSLYSALLVSACEADIIALVLNADAPWSPFSPGFTSSMNRPVIGVVTKADLA
                     NMEQISLVKCWLREAGAHNVLVTSAVNNHGVAELLALLHTEEGCR"
     gene            complement(14709..15038)
                     /locus_tag="KLK57_000111"
     CDS             complement(14709..15038)
                     /locus_tag="KLK57_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HBE0890602.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(15210..16268)
                     /locus_tag="KLK57_000112"
     CDS             complement(15210..16268)
                     /locus_tag="KLK57_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBE0890603.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSFVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(16466..16939)
                     /gene="sbmC"
                     /locus_tag="KLK57_000113"
     CDS             complement(16466..16939)
                     /gene="sbmC"
                     /locus_tag="KLK57_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HBE0890604.1"
                     /translation="MNYEITQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDNKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPNNFTLPENSEGVILTEISGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(17058..18224)
                     /gene="dacD"
                     /locus_tag="KLK57_000114"
     CDS             complement(17058..18224)
                     /gene="dacD"
                     /locus_tag="KLK57_000114"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HBE0890605.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALADYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVFSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIDLGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVAHWPLVTLESVGEGGMFSRLSDYFHHKA"
     gene            18433..19860
                     /gene="sbcB"
                     /locus_tag="KLK57_000115"
     CDS             18433..19860
                     /gene="sbcB"
                     /locus_tag="KLK57_000115"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HBE0890606.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDNEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVCATIAMAKLVKTRQPRLFDYLFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMVDLAGDISPLLELDS
                     DTLRERLYTAKADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(19903..20130)
                     /locus_tag="KLK57_000116"
     CDS             complement(19903..20130)
                     /locus_tag="KLK57_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HBE0890607.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(20144..21202)
                     /gene="yeeE"
                     /locus_tag="KLK57_000117"
     CDS             complement(20144..21202)
                     /gene="yeeE"
                     /locus_tag="KLK57_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HBE0890608.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAEAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYSTEHNSIAETFNLSVWPLVAVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCFIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(21381..22739)
                     /gene="plaP"
                     /locus_tag="KLK57_000118"
     CDS             complement(21381..22739)
                     /gene="plaP"
                     /locus_tag="KLK57_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HBE0890609.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(22729..22791)
                     /gene="yoeI"
                     /locus_tag="KLK57_000119"
     CDS             complement(22729..22791)
                     /gene="yoeI"
                     /locus_tag="KLK57_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HBE0890610.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(23006..23935)
                     /locus_tag="KLK57_000120"
     CDS             complement(23006..23935)
                     /locus_tag="KLK57_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0890611.1"
                     /translation="MKQLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLVQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(23981..24805)
                     /locus_tag="KLK57_000121"
     CDS             complement(23981..24805)
                     /locus_tag="KLK57_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0890612.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(24888..25142)
                     /gene="yoeB"
                     /locus_tag="KLK57_000122"
     CDS             complement(24888..25142)
                     /gene="yoeB"
                     /locus_tag="KLK57_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HBE0890613.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(25139..25390)
                     /gene="yefM"
                     /locus_tag="KLK57_000123"
     CDS             complement(25139..25390)
                     /gene="yefM"
                     /locus_tag="KLK57_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HBE0890614.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            25673..25723
                     /gene="hisL"
                     /locus_tag="KLK57_000124"
     CDS             25673..25723
                     /gene="hisL"
                     /locus_tag="KLK57_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HBE0890615.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    25699..25822
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            25869..26768
                     /gene="hisG"
                     /locus_tag="KLK57_000125"
     CDS             25869..26768
                     /gene="hisG"
                     /locus_tag="KLK57_000125"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HBE0890616.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            26774..28078
                     /gene="hisD"
                     /locus_tag="KLK57_000126"
     CDS             26774..28078
                     /gene="hisD"
                     /locus_tag="KLK57_000126"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HBE0890617.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     ARGDDALREYSAKFDKTTVNALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCGVKDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MAHQVAEAVERQLAELPRAETARQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARDLVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNALKEQA"
     gene            28075..29145
                     /gene="hisC"
                     /locus_tag="KLK57_000127"
     CDS             28075..29145
                     /gene="hisC"
                     /locus_tag="KLK57_000127"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HBE0890618.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTQDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLTEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVTQIIAER
                     EYLIAALKEIPCVEQVFDSATNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            29145..30212
                     /gene="hisB"
                     /locus_tag="KLK57_000128"
     CDS             29145..30212
                     /gene="hisB"
                     /locus_tag="KLK57_000128"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HBE0890619.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDREILNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKMALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            30212..30802
                     /gene="hisH"
                     /locus_tag="KLK57_000129"
     CDS             30212..30802
                     /gene="hisH"
                     /locus_tag="KLK57_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HBE0890620.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            30802..31539
                     /gene="hisA"
                     /locus_tag="KLK57_000130"
     CDS             30802..31539
                     /gene="hisA"
                     /locus_tag="KLK57_000130"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HBE0890621.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPERVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            31521..32297
                     /gene="hisF"
                     /locus_tag="KLK57_000131"
     CDS             31521..32297
                     /gene="hisF"
                     /locus_tag="KLK57_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HBE0890622.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDGETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            32291..32902
                     /locus_tag="KLK57_000132"
     CDS             32291..32902
                     /locus_tag="KLK57_000132"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HBE0890623.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVNIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGDTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLRKRHQ"
     gene            complement(32999..33976)
                     /gene="wzzB"
                     /locus_tag="KLK57_000133"
     CDS             complement(32999..33976)
                     /gene="wzzB"
                     /locus_tag="KLK57_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HBE0890624.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVAKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIENLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(34122..35288)
                     /gene="ugd"
                     /locus_tag="KLK57_000134"
     CDS             complement(34122..35288)
                     /gene="ugd"
                     /locus_tag="KLK57_000134"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HBE0890625.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRDADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVPVGFTEAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLAIFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            complement(35538..36944)
                     /gene="gndA"
                     /locus_tag="KLK57_000135"
     CDS             complement(35538..36944)
                     /gene="gndA"
                     /locus_tag="KLK57_000135"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HBE0890626.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(37108..38478)
                     /gene="cpsG"
                     /locus_tag="KLK57_000136"
                     /gene_synonym="manB"
     CDS             complement(37108..38478)
                     /gene="cpsG"
                     /locus_tag="KLK57_000136"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HBE0890627.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALARGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPFEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEEKTKLI
                     LELLNK"
     gene            complement(38583..40019)
                     /gene="cpsB"
                     /locus_tag="KLK57_000137"
     CDS             complement(38583..40019)
                     /gene="cpsB"
                     /locus_tag="KLK57_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HBE0890628.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGAMHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(40022..41245)
                     /gene="wcaI"
                     /locus_tag="KLK57_000138"
     CDS             complement(40022..41245)
                     /gene="wcaI"
                     /locus_tag="KLK57_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HBE0890629.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGSKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLDLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(41242..41721)
                     /gene="gmm"
                     /locus_tag="KLK57_000139"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(41242..41721)
                     /gene="gmm"
                     /locus_tag="KLK57_000139"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HBE0890630.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELILPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(41724..42689)
                     /gene="fcl"
                     /locus_tag="KLK57_000140"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(41724..42689)
                     /gene="fcl"
                     /locus_tag="KLK57_000140"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HBE0890631.1"
                     /translation="MSKQRIFIAGHRGMVGSAIRRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRS
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            complement(42692..43813)
                     /gene="gmd"
                     /locus_tag="KLK57_000141"
     CDS             complement(42692..43813)
                     /gene="gmd"
                     /locus_tag="KLK57_000141"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HBE0890632.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(43839..44387)
                     /gene="wcaF"
                     /locus_tag="KLK57_000142"
     CDS             complement(43839..44387)
                     /gene="wcaF"
                     /locus_tag="KLK57_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HBE0890633.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKS
                     LPANVVCRGNPAVVIRERVETE"
     gene            complement(44403..45149)
                     /gene="wcaE"
                     /locus_tag="KLK57_000143"
     CDS             complement(44403..45149)
                     /gene="wcaE"
                     /locus_tag="KLK57_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HBE0890634.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLAQAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            complement(45160..46377)
                     /gene="wcaD"
                     /locus_tag="KLK57_000144"
     CDS             complement(45160..46377)
                     /gene="wcaD"
                     /locus_tag="KLK57_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HBE0890635.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVIGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFRVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            complement(46352..47569)
                     /gene="wcaC"
                     /locus_tag="KLK57_000145"
     CDS             complement(46352..47569)
                     /gene="wcaC"
                     /locus_tag="KLK57_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HBE0890636.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLKSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVDEEDVLQLVQLSKPEIAQAIFGTTLAEFSQRSRATYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(47566..48054)
                     /gene="wcaB"
                     /locus_tag="KLK57_000146"
     CDS             complement(47566..48054)
                     /gene="wcaB"
                     /locus_tag="KLK57_000146"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HBE0890637.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(48057..48896)
                     /gene="wcaA"
                     /locus_tag="KLK57_000147"
     CDS             complement(48057..48896)
                     /gene="wcaA"
                     /locus_tag="KLK57_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HBE0890638.1"
                     /translation="MKENPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            complement(48989..51151)
                     /gene="wzc"
                     /locus_tag="KLK57_000148"
     CDS             complement(48989..51151)
                     /gene="wzc"
                     /locus_tag="KLK57_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HBE0890639.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWERLMGRQNETVKVTTFNRPKEMAGQVFTLNVLDNK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVSWASKNYDLVLIDTPPILAVTDAAIVGRHAGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(51154..51597)
                     /gene="wzb"
                     /locus_tag="KLK57_000149"
     CDS             complement(51154..51597)
                     /gene="wzb"
                     /locus_tag="KLK57_000149"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HBE0890640.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(51603..52742)
                     /gene="wza"
                     /locus_tag="KLK57_000150"
     CDS             complement(51603..52742)
                     /gene="wza"
                     /locus_tag="KLK57_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HBE0890641.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            53051..53200
                     /locus_tag="KLK57_000151"
     CDS             53051..53200
                     /locus_tag="KLK57_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890642.1"
                     /translation="MVIEYWLSNNAGGSVTANGIAGKVTIRQLAIFKNYIKCHSIWFL
                     GVSLG"
     gene            53401..54984
                     /locus_tag="KLK57_000152"
     CDS             53401..54984
                     /locus_tag="KLK57_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBE0890643.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPRQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIVGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLHKDGELEYEV"
     gene            complement(55258..57111)
                     /gene="asmA"
                     /locus_tag="KLK57_000153"
     CDS             complement(55258..57111)
                     /gene="asmA"
                     /locus_tag="KLK57_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HBE0890644.1"
                     /translation="MRRFLTTLMILLVVLVAGLSVLVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQAQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(57133..57714)
                     /gene="dcd"
                     /locus_tag="KLK57_000154"
     CDS             complement(57133..57714)
                     /gene="dcd"
                     /locus_tag="KLK57_000154"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HBE0890645.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(57806..58447)
                     /gene="udk"
                     /locus_tag="KLK57_000155"
     CDS             complement(57806..58447)
                     /gene="udk"
                     /locus_tag="KLK57_000155"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HBE0890646.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            58765..62082
                     /locus_tag="KLK57_000156"
     CDS             58765..62082
                     /locus_tag="KLK57_000156"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBE0890647.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLALGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWERELKPN
                     VFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRILLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMIAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQTFRRSFS
                     DPALSHALDRRVFHEFFQQAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPAEAILDHAESVQKLQLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(62192..63040)
                     /gene="alkA"
                     /locus_tag="KLK57_000157"
     CDS             complement(62192..63040)
                     /gene="alkA"
                     /locus_tag="KLK57_000157"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HBE0890648.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADNYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLLDLQCNPQIVNGALGKLGAGR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKRRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDEA"
     gene            63174..64526
                     /gene="yegD"
                     /locus_tag="KLK57_000158"
     CDS             63174..64526
                     /gene="yegD"
                     /locus_tag="KLK57_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBE0890649.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQEILERAAKRAGFRDVVFQYEPIAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISNELATLISQRGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPQIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(64539..66479)
                     /gene="yegI"
                     /locus_tag="KLK57_000159"
     CDS             complement(64539..66479)
                     /gene="yegI"
                     /locus_tag="KLK57_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HBE0890650.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYS
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTENHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVSALDSLRQQLKKCTVSAMHVYPGHLADCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ANCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESALTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(66476..67237)
                     /gene="pphC"
                     /locus_tag="KLK57_000160"
     CDS             complement(66476..67237)
                     /gene="pphC"
                     /locus_tag="KLK57_000160"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HBE0890651.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVVWLNDQQPLLVMFL
                     ADGAGSVSQGGEGATLAVNEAMAFMVQKVQDGELGLNDVLATNMVLTIRQRLFAEAEA
                     KALAVRDFACTFLGLISSPDGTLIMQIGDGGVVVDLGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTGRAHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(67234..67893)
                     /locus_tag="KLK57_000161"
     CDS             complement(67234..67893)
                     /locus_tag="KLK57_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBE0890652.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VTFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(68114..68173)
                     /gene="ibsA"
                     /locus_tag="KLK57_000162"
     CDS             complement(68114..68173)
                     /gene="ibsA"
                     /locus_tag="KLK57_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HBE0890653.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(68446..68502)
                     /locus_tag="KLK57_000163"
     CDS             complement(68446..68502)
                     /locus_tag="KLK57_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBE0890654.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(68773..68829)
                     /locus_tag="KLK57_000164"
     CDS             complement(68773..68829)
                     /locus_tag="KLK57_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBE0890655.1"
                     /translation="MMKLLIIVVLLVISWPAY"
     gene            69108..70349
                     /gene="mdtA"
                     /locus_tag="KLK57_000165"
     CDS             69108..70349
                     /gene="mdtA"
                     /locus_tag="KLK57_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HBE0890656.1"
                     /translation="MKGSYKSRWVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQQS
                     PAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTIIAANTVTVRSRVDGQLMALHFQ
                     EGQQVKAGDLLAEIDPSQFKVALAQTQGQLAKDKATLANARRDLARYQQLAKTNLVSR
                     QELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQISSG
                     DTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKTLVVEAWDRTNSKKLSEGTLLS
                     LDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNEGH
                     FVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVEAQ
                     SATTPEEKATSREYAKKGARS"
     gene            70349..73471
                     /gene="mdtB"
                     /locus_tag="KLK57_000166"
     CDS             70349..73471
                     /gene="mdtB"
                     /locus_tag="KLK57_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HBE0890657.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGRGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFTNMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDQGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTEITPGLAALDTIRLTSSDGGVVPLSSIAKV
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQEIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            73472..76549
                     /gene="mdtC"
                     /locus_tag="KLK57_000167"
     CDS             73472..76549
                     /gene="mdtC"
                     /locus_tag="KLK57_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HBE0890658.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRHLLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            76550..77965
                     /locus_tag="KLK57_000168"
     CDS             76550..77965
                     /locus_tag="KLK57_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0890659.1"
                     /translation="MTELPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLLLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLITLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            77962..79365
                     /gene="baeS"
                     /locus_tag="KLK57_000169"
     CDS             77962..79365
                     /gene="baeS"
                     /locus_tag="KLK57_000169"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HBE0890660.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDEQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            79362..80084
                     /gene="baeR"
                     /locus_tag="KLK57_000170"
     CDS             79362..80084
                     /gene="baeR"
                     /locus_tag="KLK57_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HBE0890661.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
BASE COUNT        19330 a        21223 c        20480 g        19134 t
ORIGIN      
        1 cgattaagtc agccattctt tttcctcgct tttttttata gtcatatcgt caaaaaaatc
       61 caggcgaggt ctttatgcaa caagaagcgt taggaatggt agaaaccaaa ggcttaactg
      121 ccgccataga ggccgcagac gccatggtga agtcggcaaa tgttttgctg gtcggctacg
      181 aaaaaatagg ttccgggctg gtgaccgtca tcgttcgtgg tgatgtcggg gcggtaaaag
      241 cggcaacaga cgcgggtgcc gcagctgcgc ataacgtcgg cgaagtaaaa gccgtacacg
      301 ttattccccg cccacatacc gatgtcgaaa aaatcttacc aaagggaatt aacccatgag
      361 cagcaatgaa ctggtcgaac agatcatggc gcaggtcatc gctcgcgtag cgacgccaga
      421 gcatgaggtt ctcccgaaaa ccactcatca aatacgagag acggctatgg cagaaaaaag
      481 ctgcacgcta acagaatttg ttggcaccgc tattggcgac actgtaggtc tggtcattgc
      541 gaacgttgat agcgccctgt tggacgcgat gaaacttgaa aagcgctatc gttccattgg
      601 cattctgggt gcgcgtattg gcgcagggcc acacattatg gccgctgatg aagcggtaaa
      661 agccaccaac actgaagtta ttagcattga acttcctcgc gatactaaag gcggtgccgg
      721 acacggctca ctgatcctct taggtggcaa cgatgtttcc gacgtgaaac gcggtattga
      781 agtcgccctg aaagaactcg atcggacctt tggcgatgtc tatgccaacg aagccggaca
      841 tcttgaactg caatataccg cccgcgccag ctatgcactg gaaaaagcgt ttggtgcacc
      901 actgggacgg gcttgcggcg ttattgtcgg cgctccggct gctgtgggtg ttctgatggc
      961 cgacaccgcg ctgaagtccg ccaatgtgga tgttgtggca tacagctccc cagctcaaga
     1021 caccagctac agcaatgaag tcattctgat tatctcgggt gactccggcg cagtccgcca
     1081 ggcggtcatc tccgcacgcg aaatcggtaa aaccgtgctt tcgactctcg gcgatacacc
     1141 gaaaaacgat cacccttcct acatctgaat tctctgaggc agatttatga gatcgaaaag
     1201 atttgaagca ctggcaaaac gcccggtcaa ccaggacggt ttcgttaaag agtggattga
     1261 agaaggcttt attgcgatgg aaagcccgaa cgatcctaaa ccgtcaatca aaattgttaa
     1321 cggcgtcgtt accgaactgg atggaaaacc cgccagccag tttgacctga tcgaccactt
     1381 tatcgcccgc tacggcatca atctcgcgcg cgccgaagaa gtcatcgcga tggattcggt
     1441 aaaacttgcc aatatgttgt gcgatccaaa tgtcaaacgt agcgatatcg ttccccttac
     1501 cacagcaatg acgcctgcaa aaatcgtcga agtggtttcg cagatgaacg tggtagagat
     1561 gatgatggcg atgcagaaaa tgcgcgcccg tcgaacacca tcacaacagg ctcacgtcac
     1621 caacgtcaaa gataacccag tacaaattgc cgccgacgca gctgaaggcg catggcgcgg
     1681 gtttgatgaa caggaaacca ccgttgccgt tgctcgctat gcaccgttca acgccatcgc
     1741 cctgctggtc ggctcacagg tgggacgtcc gggcgtcttg acccagtgtt cactggaaga
     1801 agccaccgaa ctgaaactcg gtatgttagg ccacacctgt tacgccgaaa caatttctgt
     1861 ctatggtaca gaacctgttt tcaccaatgg tgatgatacc ccgtggtcaa aagggtttct
     1921 cgcgtcatcc tatgcttctc gtggcttaaa aatgcgcttt acttccggct ccggctctga
     1981 agtacagatg ggttacgcgg aaggcaaatc aatgctttac ctcgaggctc gttgcattta
     2041 catcaccaaa gccgcaggcg ttcagggtct gcagaatggt tcggtaagct gcatcggtgt
     2101 tccatcagct gtgccatcag gcatccgcgc agtattagcg gaaaacttaa tttgctcatc
     2161 gctggatctg gaatgcgcct ccagtaatga ccagaccttt acccactcgg atatgcgccg
     2221 taccgcacgt ttcctgatgc aatttctgcc aggcaccgac tttatctctt ccggtttttc
     2281 cgctgtgccg aactacgaca acatgtttgc tggttcaaac gaagatgctg aagacttcga
     2341 tgattacaac gtcattcagc gtgacctgaa ggttgatggt gggctgcgac cagtacgcga
     2401 agaagatgtt atcgctatcc gcaataaagc cgctcgcgct ttacaggctg tctttgccgg
     2461 tatgggacta ccgcctatta ccgatgaaga agtcgaagcg gcaacctatg cccacggttc
     2521 aaaagatatg cctgagcgca atattgtgga agacatcaag ttcgcccagg agattatcaa
     2581 taaaaaccgg aacagcctgg aagtggtgaa agccttggcg caaggcagtt ttaccgatgt
     2641 cgcccaggac atgctcaaca tgcaaaaagc aaaactgacc ggcgattatc tccatacctc
     2701 agccatcatc gtcgatgacg gacaagtgct ctctgcggtc aatgacgtca atgattacgc
     2761 cggaccggct acaggttacc gcctgcaagg ggaacgctgg gaagagatca aaaatattcc
     2821 cggtgcactt gatcccaacg aaattgacta agggtgagaa atggaaatta acgaaaaatt
     2881 actgcgtcag attatcgaag acgtgctggc agaaatgcaa cccagcgaca agtccgtttc
     2941 atttcgcgca cctgtctctg caactgtccc ttccgcgcca gataccggaa attttctgac
     3001 cgaaattggc gaagcccaac aaggcactca gcaagatgaa gtcattattg ctgtaggccc
     3061 ggcgttcggc ctggcgcaaa ccgtcaatat catcggtatt ccgcataaaa acatcttgcg
     3121 cgaagtgatt gccggtatcg aagaagaagg cattaaagcc cgtgtaattc gctgctttaa
     3181 atcgtccgat gtcgcattcg tagcagtcga aggcgatcgt cttagtggtt caggtattgc
     3241 tatcggtatt cagtcgaaag gcacaacggt tatccaccag cagggacttc ctcccttgtc
     3301 taacctggaa cttttcccac aagcgccttt gttgacgctg gatacttatc gtcagattgg
     3361 caaaaacgcc gcgcgttatg cgaagcgcga gtctccacaa ccggtgccga ccctcaacga
     3421 tcaaatggcg cggccgaaat accaggccaa atctgccatt ttgcatatta aagagacgaa
     3481 gtacgtggtc actggcaaaa aaccgcaaga actgcgcgtg acgttttaac aaaggaaacc
     3541 ttcatgaata ccgatgcaat tgagtcgatg gtcagggacg tgttgaaccg catgaacagt
     3601 ttacaggatg cagctcctgt ttctgcggtc cccaacgcat caatactgag cgccaaagtg
     3661 acggattacc cgctggcaaa taaacatccg gaatgggtta aaacagcgac caataaaacg
     3721 ctcgacgatt tcactctgga aaatgtgcta agcgataacg tcaccgccct ggatatgcgc
     3781 attacccctg aaacattgcg tattcaggct gcgattgcca gagatgctgg tcgcgatcgt
     3841 ctggcgatga attttgaacg cgccgctgag cttacctcag taccagatga tcgcatcctc
     3901 gaaatctaca atgcgttacg cccttatcgt tcaactaagc aagaacttat tgccattgct
     3961 gacgatcttg aacagcgtta tcaggccaaa atttgtgctg catttgtccg tgaagctgcg
     4021 gaactttatg tcgagcgcaa aaaactgaaa ggcgacgatt aaggacgaac tatgcgatat
     4081 atagcaggca ttgatatcgg caactcatca acagaggttg cgctggcaac gctcagtgca
     4141 actggagagt tgtcgttcgt gagtagcgcc ctggaggaaa ccaccggaat caaaggtacg
     4201 ttgcgtaacg tccatggtat tcaggaggcg ctcgcacagg caacgaaaaa agtcggcatc
     4261 aatgttagcg atatttcgct tattcgtatc aacgaagcaa ctccggtcat tggtgatgtg
     4321 gcgatggaaa ccatcactga aactatcatc accgaatcaa ccatgatcgg tcacaacccg
     4381 aaaactccgg gcggagtggg gctgggcgta gggttcacca tcactccgca agagttatta
     4441 acacgcccgg cggacacacc ttacatcctg gtggtgtcat cggcatttga ttttgccgat
     4501 atcgcgacga tgataaacgc ctccgtacgc gccggatatc agctgactgg cgttattctg
     4561 caacgcgacg atggcgtact ggtcaataac cgcctggaaa ttcctttacc tattgtggat
     4621 gaagtcctct acatcgatcg tattccgctc ggcatgctgg cagcaattga agttgccgtt
     4681 cccgggaaag ttatcgaaac cctgtctaac ccgtatggga ttgccaccgt attcgccctc
     4741 aatgctgaag agactaaaaa tattgttcct gtcgcgcggg cgctaatcgg caaccgttca
     4801 gcagtggtag tgaaaacgcc ctctggcgac gttaaagccc gttcgatacc cgccggaaat
     4861 atcgaactgc tttctgccgg gcgcacaacg cgcgttgatg tagctgctgg tgccgatgcc
     4921 attatgaaag cggtcggtga gtgcccaaaa ctggaaaacg tgacgggaga accaggcacc
     4981 aacatcggcg gaatgctgga acatgttcgc cagaccatgg cggaactgac caacaaaccc
     5041 agcaacgaaa tttttattca ggatttgctg gcgatagata cctctgtccc cgtcagcgta
     5101 accggtggtc tggcaggtga attttcactg gaacaggctg tgggtattgc ctctatggtg
     5161 aaatccgacc gtctgcaaat ggcgatgatc gccagtgaaa ttaagcaaaa actgcatgtt
     5221 gatgttcagg tcggaggcgc agaagcagaa gccgccattc agggggcctt gactacccct
     5281 ggaaccacac gtccactggc aattctggac ttaggtgcag gctctaccga tgcctccatc
     5341 atcaatcaga gtggggaaat cgttgccact caccttgcgg gagcgggcga catggtaacg
     5401 atgattatcg ctcgtgagct gggcttaaat gatcgttatc tggcagaaga aatcaaaaag
     5461 tacccgttag caaaagtaga aagcctgttc cacttacgcc atgaagacgg tagcgttcag
     5521 ttcttcccta ctcccctatc cccccatgta tttgcccgcg tctgtgtagt gaaacccgac
     5581 gaactggtgc ctattcccgg tgatttgacg ctggaaaaag tacgcgctgt tcgtcgcagt
     5641 gcgaaagagc gtgtcttcgt aaccaatgcc ctgcgcgcgc tacgtcaggt aagccctgcc
     5701 ggaaatattc gcgacattcc gttcgtcgtt ctggtgggtg gttcttcgct ggatttcgaa
     5761 gtaccgcaat tggtcaccga cgcgctggca cattatcgac tggtcgccgg gcgggggaat
     5821 atccgtggta gcgaaggtcc gcgcaacgcg gtagctactg ggctactcat cgcctggcac
     5881 aaggagtcga ttcatggaaa gtaaagagcg tgcaccagcc atcgttgtca tggagatcgg
     5941 tgactgtatc actacatggg atgaggtgct tcttggcatt gaagaggaag gtattccctt
     6001 tcgcattcaa cacattccgt caggtgaagt tatcgatagc gcctggctgg ctgctcgtca
     6061 atctcccctg ttggttggca ttgcctgcga ccgggaagaa ctgattgtgc attacaaaaa
     6121 cttaccggca tcagcgccac tgtttacgct gacgtatcaa caagataacc atacccggcg
     6181 cagcatcggc actaacgctg cccgcctggt aaaaggaatt cccttccggg aattccactc
     6241 ataatccaca ggagaaacgc tgtatgaaca acgcactagg actggttgaa acaaaagggt
     6301 tagttggcgc aattgaagct gcagatgcca tggttaaatc cgccaacgtg cagctggttg
     6361 gctatgaaaa gattggttcg ggtctgatta cggtgatggt ccgcggtgat gttggcgcag
     6421 taaaggccgc cgttgatgcg gggagcgctg cggcaagcgc cgttggcgaa gtgaaatcct
     6481 gccatgtcat cccgcgccca cacagtgatg ttgaggctat tttacctaaa tcggcataac
     6541 cccatttgct aaggagcaca gcgtgaagca atcactggga ttacttgaag ccagtggtct
     6601 ggctctcgcc atcagttgcg cagacgctat ggcaaaagcc gcgtccatca cgctggttgg
     6661 ccttgagaag accaatggct caggctggac gctcatcaaa atcaccggtg acgtcgcctc
     6721 ggttaatgcg gcgatcgcga cgggagccag tttcgccgga cagcgaaatg gccttgtcgc
     6781 acacaaagtg attgcccgcc cgggagacgg cattctgccc cacacaactg aagcattgac
     6841 cgtgacggtt gaaacaagca aaacaccagc aacaaccgtc acctcagaga ttatcagttg
     6901 taatttgtgt cttgacccgg catgtcagcg gcaaaaaggt gagccccgct cactttgcct
     6961 gtacgccaat aagcgagggg agtagcagtg gataaacaaa ccttgcagga aacagtgagc
     7021 cagattctgg atgagatgcg cgagcgccct atcccactgg gcgtttccag tcgccatata
     7081 catctttcat cagcggatta tttgcgtctt tttcctgaac aaccgattag cgagaaaaag
     7141 ccgttaatgc aacctggcca atacgctgct gaacaggttg ttacgctgga gggaccaaaa
     7201 ggcaaattgc acaacgttcg cttgttgggg ccgctacgca atgcaagcca ggtggaaatt
     7261 tcccggacgg acgccagaac gttagggatc accgccccgc tacgcatgtc aggcgatctc
     7321 agcggcactc cggggattcg tttgataagc ccatttgcgg aactggagat cacatctggc
     7381 gtcattgtcg cgcaacggca tattcatatg tctccgctgg atgccctgat ttatggcgtt
     7441 acgcatggcg ataaagtggc ggttgccatt gaaggtgatg agcgacgact gatattcgaa
     7501 aacgtcgctg ttcgcgtctc tccggatatg cgtcttgaaa tgcatattga taccgatgag
     7561 gccaacgccg caggcgctga tagccctgac gtttttgcca cactggtgtc agtgccatga
     7621 ataacatgct gatgcaacgt attgttgacg aagtggtttt tcgccttaaa caacgtgcgg
     7681 gaaaaacact cgtgctgact gtttttcaat tgcgagacgc cagcgttcaa gagagcgttc
     7741 atcagtatgc gtcattacaa atccgatatg tcgatctgcc gctgctgcga caacttgcgg
     7801 agaacgaaac gtcagatcga gcatctatcc agatacacga agcgttagcc tggggaatgc
     7861 acattcaatt gtcgttgcag cgtcatttcc tgaacgccat cgaattgaag actctcgccc
     7921 gtctaccgct aagctggtgc gacgaacaag ggcagccgat ctaccttcat cataatcgac
     7981 ttttaagtta tgccgatatc gcacagttaa gctcgggaat actggtgtta cagcggaaat
     8041 gttgtgtgac cgcactggct cgtgaagcgg caattacccg aaatattcag ttaatcaggc
     8101 aggagtaacc tatgcatctg gctcgagtca ctggcgcggt agtatccacg caaaaatcac
     8161 cttctctgat cgggaaaaaa ctgctgctgg ttcgccgggt cagtgctgac ggcgaactgc
     8221 ctctcacgcc attaaccggc gacgaagttg ccgttgattc agtcggcgca ggcgttggtg
     8281 agttggtact cctcagcagt ggttccagcg ccagacatgt gttttcaggc cctaacgagg
     8341 ccatcgacct ggcagtggtc ggcattgtcg atacgctttc gcgctaagga gctaaaagat
     8401 ggcaatttat acccgaacag gtgattcagg aagtacgtct ttatttaccg gacagcgcgt
     8461 cagcaaaact catctacgtg tcgaaaccta tggcacgctg gatgaactga acgcgacact
     8521 cagtctgtgt tattgcgcca ccgccatcga aagccatcgc atcttactgg aagccattca
     8581 acaacagata ttttggttta gcgccgaact cgccagtgaa agcgaacagc cgtcagcgca
     8641 acagcgttac atcggtacgg aagaaattgc cgcgttggaa aacgcgatcg atagtgcaat
     8701 gaacgccgtc cccccagttc acagcttcat tctgcctggt cgatgtgaag ccgcaagccg
     8761 catgcatttc gcccgcacgg tggctcgacg ggctgaaaga cggctggttg aactgacaac
     8821 ggaaaccacc gtccggaatg ttttgctgca ctatatcaac cgactttccg attgcctgta
     8881 tgccctcgcc cgcgtggaag acaacgttgc tcatcaaaac ctgatgattc aggaaatcac
     8941 aaaacgttat cacgaagcca accacacacc agcattgaag gaacgcacaa tgccgctcac
     9001 ttttcaggat cttcaccagc tcattcgtag tgcggcaatg cgcgcggatg aactgcatat
     9061 tcccgttgtc atcagcattg ttgatgccaa cggaacagaa agtgttacct ggcggatgcc
     9121 tgatgccctg ttggtcagca gtgaactggc accgaaaaaa gcctggaccg ccgtggcgat
     9181 gaaaacggca acccacaagc ttgctgatac cgtgcaaccc gacgcgccgc tttacgggct
     9241 cgaaagccat atgcaaggaa aagtcgtcac ttttggtggc ggcttccccc tctggcgtga
     9301 cggaaaattg cttggcgggc ttggcatcag cggcggtagc gttgaacaag acatggatat
     9361 tgcccaaagc gcaatggcgg caattaacgt gggagtaaac caatgaataa ctctgaatta
     9421 gaaacactga tccgcaatat tctgcgtgag caacttgtcc ccgctacacc acgaacgcaa
     9481 agaaacgcta ttttccagac tgtcgatgaa gcggtctgcg cagcacacta ggccttttta
     9541 cgctttcaac agtgcccgct taaaacccgt agcgccatta tcagcgccat tcgcgaagaa
     9601 ttaatgcccc gccttaccga actggcagaa gaaagcgcca aagaaacggg gatgggaaat
     9661 aaagaagata agtacctaaa aaacaaagcg gcactcgata acacgccagg aattgaagat
     9721 ttaaccacca cagcactaac cggtgatggt ggcatggttt tgtttgagta ctctccgttt
     9781 ggggtgattg gctcggtcac gccaagcacc aaccccacgg aaaccatcat taacaacagc
     9841 atcagcatgc tggcagcagg taatagcgtc tacttcagtc cacacccggg tgccaaaaac
     9901 gtttcgttaa aactgattac cacaatcgaa gacatcgcgt ttcgctgtag tggcattcat
     9961 aacttgatcg ttaccgtatc tgaaccgacc tttgctgcca ccgaacagat gatggcgcac
    10021 cctaaaatca gcgttctggc gattaccggc ggtccgggga tcgtcgccat gggaatgaaa
    10081 agtggcaaga aagtgattgg tgccggagcg ggtaaccctc cgtgcatcgt cgatgaaacg
    10141 gcggatattg tgaaagccgc cgaggacatc atcaacggcg cttcatttga ttacaacctg
    10201 ccgtgtattg ctgaaaaaag cctgattgtc gtggcatcag tggcagatta tttaattcag
    10261 cagatgcaaa gttttggcgc acttcttctt aactatgagc aaactgagaa attgcgtgca
    10321 atttgcctgc cagacggtag cgccaacaaa aaactggttg ggaaaagccc atcagccttg
    10381 ctggaagcgg caggcctccc tttgcccgca aaagcgccgc gcctactgat tgctgttgtc
    10441 gacgccgatg atagttgggt tacttgtgag caactaatgc caatgctgcc cattgtgaag
    10501 gtcaaggatt ttgacagcgc tctaaccctg gcgctgaaag tggaagacgg cttgcaccac
    10561 acggcaatta tgcactctca gaacgtctca cgactcaacc ttgccgcgcg cgttatgcaa
    10621 acctcaatat ttgtcaaaaa cggcccctcc tgggcgggta ttggcgtcgg cggcgaaggc
    10681 tttacgacct ttaccattgc aacaccgact ggagagggta caacgtcagc acggactttt
    10741 gcccgctcac gacgctgcgt gttaaccagc ggcttttcaa ttcgctagag aaacggaaat
    10801 gaatactttt tcactgcaga cgcgtttata cagcggacca ggaagtctca aggtacttag
    10861 ccgtttctct catagacaca tctggattat ttgcgatgcc tttctggcac gctcaccact
    10921 tattgacacg ttgcatcagg cgctgcccga cagtaatcgt ctcagtattt ttagcgaaat
    10981 aacgcccgcc ccaactatcc agaccgtggt gaaaggcatt gcgcaaatgc agactttacg
    11041 cccggatgta gttatcggtt ttggcggcgg ttctgccctg gacgccgcaa aagcaattgt
    11101 ctggttcggt cgccagtgtg gcattgaaat tgaaacctgc gtagccattc ctaccaccag
    11161 cggtatcggt tccgaagtga ccagtgcttg tgtcatcagc gatcctgaga aagggattaa
    11221 atatcccctt ttcgataatg cactttatcc ggatattgcg atccttgatc catctttggt
    11281 cgtcagcgtg ccaccggcga taacggccaa taccgggatg gatgttttaa cccatgcgct
    11341 tgaagcttat gtttctcctc gcgccagcga cttcacggat gcactggtcg aaaaagccgt
    11401 gcaaattgtt tttcaatacc tgcccactgc agtgaaaaaa ggcgattgtc ttgccacccg
    11461 tggcaaaatg cataatgcat cgacgcttgc aggaatggcc tttagccagg ccggactcgg
    11521 tttaaaccat gcaattgctc atcaactcgg cggacagttt catctgccgc atggactggc
    11581 gaatgcgctc ctgctcacgg cagttatccg ctttaatgct ggcgatccgc gcacagccaa
    11641 acgctatgcc cggctggcga aaacgtgcca tttatgcccg gacaacgcca atgacaccgc
    11701 cagcctgaat gcacttattc aacatattga gcagcttaat acaacctgta cgctgccgac
    11761 tcttgccaat gcgctgaaag aaaaaaaagc agaatggtca atacgcatac cagatatggt
    11821 tcaggcggcg ctggcggatg caacgttgcg taccaacccg cgtgcagctg atgcctccgc
    11881 gattgcagaa ctgctcaagg ggttgttatg aacgtcggat taatctcctg cgacgaccag
    11941 accatccgcg agcgagtaca agcagcaggt gtcgtgggtg ctggcggcgc aggttttcca
    12001 acccatatta agctacaagc gcgtgctgac actttcctgg tgaatgcggc ggaatgcgaa
    12061 cccatgttga aagtagatca acagttaatg gcgcagcaag ccagccgcct gcttcgcggt
    12121 gttcactatg cgatgaaagc caccggggcc agtagcggaa tcatcgcgct gaaagaaaaa
    12181 tatcaacggg caataaacgc actcacacca ctgttaccac cggatatccg cctgcatatt
    12241 ttgcccgacg tttaccccgc aggagacgaa gtgctgacca tctggatggc aactggtcgc
    12301 cgcgttccac ccgctgcatt acccgttagc gtcggtgtgg tggtcaataa tgttcaaacc
    12361 gtacttaata ttgccagagc cgtcgagcaa cagtatccag tgacgcgccg cacactcacc
    12421 gttaatggtg cagtggcgaa gccaataacg gtaaccgtcc ccatcggcat gtctttacgt
    12481 gaagtcctgg cgctggcggg cggcgcaaca gtggatgctc ctggttttat caatggcggg
    12541 ccgatgatgg gaagtttgat cacatcgctc gacacaccag tcagcaaaac cactggcggc
    12601 ttgctggttt tacccaactc tcattcactg atccaacgtc gaatgcagaa tgaccgcagc
    12661 gtacttgccg tcgcgaaaac tgtttgtgag caatgccgct tgtgtaccga tctctgccca
    12721 cgtcatttaa tcggtcacga actggcaccc catttattag tacgtgcgcg gttaactacc
    12781 aacagctcgc cacgccgcaa ctgttactga cagcgctcac ctgttcagag tgcaacgtct
    12841 gcgccagtgt cgcctgtccg gtaggaattt caccaatgcg cataaaccgc ctgcttaagc
    12901 aggaattacg cgcacaaaat ctccgctacg aaggtgcctt aaatcctgca gatcccatgg
    12961 caaattatcg actgattccc gtaaaacggc tggtaaccag acttggctta accccgtggt
    13021 atcaggatgc gccattgtct gaacaggtgc ctcagcccga aaaagtgacg ttattgttac
    13081 gccagcacat cggtgccagc gcaattgcgt gtgtacaaaa aggcgatcgc gtcgttcatg
    13141 ggcaatgtgt aggtcaaatt ccacacggca cattaggtgc ccccattcat gccagtattg
    13201 atggcatggt cagcgatgtg acagaaaacg cgattacgct tgtgagaggt taaccatgtc
    13261 gaaagcggta ggtattttag aacttagcag tatcgcgaaa gggatggaaa ccggcgacgc
    13321 catgctgaaa agcgccaacg ttgagttact ggtcagcaaa acgctatgcc ccggaaaatt
    13381 tctgctgatg ctcggtggcg atgtgggcgc ggtgcaacag gccatcaaca gcggagcgtc
    13441 ccttgctggt gaaatgctgg tcgacagtct tgtcctgccg aatattcatc ccagcctcct
    13501 ttctgcaatc agcggactta accatgttga acagcaccgg gctgtcggcg tagtcgaaac
    13561 ctggagtgtc gccgcctgca ttgacgctgc cgattgtgct gtcaaagctg caaatgtcat
    13621 tcttgttcgc gtacatatgg cttttggcat tggcggtaaa tgctacatgg tggttgctgg
    13681 cgatatttca gatgttaata acgcggtctc tgccgccagc gtgcgcgcgg gggaaaaagg
    13741 tttgttggtc tatcggtcgg tgattgcccg tccacatgaa gccatgtggc gtcagatggt
    13801 ggagggataa tgacaactca gcacacaact gaacggatga ttcaggaata tgtcccaggt
    13861 aagcaggtca cgctggcaca tgtcatcgcc aatccaggga aggatctgtt taagaaattg
    13921 ggtttaccag acaccatttc cgctattggt attctgacaa ttacccccag cgaagcatct
    13981 atcatcgcat gcgatatcgc cacaaaatca ggcgctgtag agatcggttt tcttgatcgt
    14041 tttaccggtg ccgtagtgct gactggtgat gtttctgcga tcgaatatgc acttagccag
    14101 gttacccgta cgctgggtga aatgatgcgc tttaccgcct gccccattac gcggacctga
    14161 cgttatgcaa cgtatgatgc tcatcggccc cagccagtgc ggaaaaacgt cactcacgca
    14221 atgtctgcgt ggcgaaaata ttcattatca gaaaacgcag gcaatcgtct ggtccccgac
    14281 caccattgat acaccaggcg aatacctcga aaaccgcagc ctctacagtg cactgttggt
    14341 cagtgcctgc gaagcggaca ttatcgcgct ggtgcttaat gcggacgcac cgtggtcacc
    14401 tttttcgcca ggctttacct cgtcaatgaa tcgcccggtc attggagtag taaccaaagc
    14461 agatttagcc aacatggaac aaatatcttt ggtgaaatgt tggttacggg aagctggagc
    14521 gcacaacgtg ttagtaacca gtgcggttaa taaccatgga gttgcagaac ttttagcctt
    14581 gctgcataca gaagaaggct gtcgctaatt tcgacaggtt atcagatggg aatgatgacg
    14641 gtgatacaca gaaaagaatc aatgagcaat gagggttgcc gggcaaccct cattgaataa
    14701 aacaggaatt acttcgcttc gccgtttttc atttcgccca tagctttcag ctttttggag
    14761 atatcgcggc gttctttgga gagctcggca tttttgatga tgtaatcgtc aacgcgatct
    14821 tcatagtcac ctttcatgct ggcgatgatg ccctggattg cttcaacgct catcccaggc
    14881 ttgatgtaat cgctcaggtt gtccagcagc aggacgcgct tctggttgtc acggatcttt
    14941 ttctcaacgt cctgaatttc acgttgcagt ttgttcttac gacggaacag acgaacaaat
    15001 tccagtacgt cctggaatga aggcttggta gtttccattt ttatacccct gataatgtga
    15061 gagtcggatt cgtttaagca accgctattc gttagggcca acattactca tagctgccgc
    15121 agatgacaat gcttttatcc cttttcacta tcataccctt tatccttgct gaatcgaagc
    15181 agcagcaaga tgattctgaa gttcaggaat tatttgcgca aggcccggca aatcagattc
    15241 gatagcaact caagctgatg cgccgtttcc atgttcagcc acacataacc atagattggt
    15301 gtttccacta ccttcaggtc atggtggtta ttgagcaact gacgcagctc ttccacagca
    15361 tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat taccttcgta cagcgcatga
    15421 acaaagctca gcagtatttg ctgcatcatg tgctgggtat cacgaagttt ttgcgcgttc
    15481 aataacacga aatggctggg gcgcgtggcc cagtacgcat tgatttgcaa ctccagcata
    15541 caaaccagat tgcggttaat ggtctggata ccttcatata tcgattttgg aatacgggtt
    15601 tctttgctgg cgggcgcaat cagcccacgc attttcacgg catcggtcag gagtttttgt
    15661 agatggcttt ccagacgtgg gcgttcgagt aagttcggtg agaatgcaga ttgatagacc
    15721 cgattatact cggtcagact tttcgccagt tgaatgcgcc agtggatgaa cgcccgttgt
    15781 ggccagatac cggtaaacaa cattgccagt aaagagccga ggatcacatc gccgcttcgc
    15841 cataacgccg tatcaatttc acctgtcggg gaaccaacaa caattgccag cgtcacccca
    15901 atcaataatc cttgatacgg tttcttgccc agcgccagcc aaccgcaaag aaacatcgcc
    15961 gccgcgcacc agactaacat cagcggtaac gaaattaact ccagttgcag agcgataaga
    16021 cctaaaatcg aacccaacac cgtaccgcca atacgttcga aggcacgagg gacaacgtta
    16081 ccccagaacg agattggccc cataatcacc accatggtga ccagcggcca ggtgctttcc
    16141 gggatagtaa acaggcggat aatgagaaaa gtgagcagga atgccagcgc gacccgagta
    16201 ccatgcacta tgcggtaatg gcggtatacc cggatttcaa acgggcttaa tgacttatcg
    16261 gcacgcacgc agaaactccg taatgaagat taaaagcgct cataactcat ctgtcggcca
    16321 gaaggcgcgc ctctgaatta tccgacggac aaaccgggaa cagtgtaatt tcccggcagg
    16381 agcgaaaaaa ttgaaaggcg catcttactc ttttcgcttt caaaaaaaga gtggtcatcg
    16441 cgttaacaca ccgccctgag atgaattagt gatgttttgg ctgcaccgca acatacattt
    16501 cgatatccca gtacccatct tccgcgccat tgttcaaata aacctcgaag cagggctttg
    16561 gtaacatttc ataagcactg tcctgcaaga ggctattaaa gaactgatac cagggtttag
    16621 caaaatcatc accgactaca cgagccaccg ccaccgcata ctgaccacct gaaatttctg
    16681 tcagaatgac gccctcactg ttttcgggaa gcgtaaagtt attcggtacc gtcacaacgg
    16741 tgtcgcagcg taatttttcg gcgggtgttt catctggatt gtcgtaatag acagcaaccc
    16801 actccttcgg cacaatattt ttgttatcta cccacatcat caactgctca aagcctttct
    16861 ttaccgtctg ttcccacggg ccaacgagat ggaaacctgc aacggtacgt ttctcttcct
    16921 gcgtaatctc gtagttcatg acgcctccat taacactgtt tttatataca gcatagttgc
    16981 aatattaaaa ccacaaggaa tgagtgttga ttatgcgagc agactcgcac tcctgccagt
    17041 ctgctgcaaa agaaaggtca ggccttatgg tggaaataat cactcaggcg ggaaaacatg
    17101 ccgccttccc cgacagattc cagggtaacc agcggccagt gcgccacctg tttatcacgg
    17161 tcgtaaagtt caatttcccc tacccgctga tgggcgctaa ttggcgcggt gagctcttta
    17221 ccatcaaggg tatatttggc tttgatatgt ggaatttcgg ctttcggtag caccatccag
    17281 aactcttgtt ccgttcctag gtcgatattt tctttatcgc cataccagat gcgttccgtt
    17341 ccgacctttt tcccacggtg caaaatttgc accgtagtaa agttttgttg cccccaacgc
    17401 agtaattttc ttgcctcttc ctcacgacct tttgcactgt cagcccccat taccactgca
    17461 atgagacgac gctgcccatc tacagccgaa gcaatgagat taaacccggc accagaagta
    17521 tgacccgttt tcaggccgtc aacattcatg gttttatccc acaacaaccc gttacggttt
    17581 tgctgggtga taccgttcca ggtgagactt ttctcactgt acatatgata aaactcgggc
    17641 tcgccgtgga tgatagcgcg agaaaacaca gctaaatcat aagccgagct atgctggcca
    17701 ggtgcatcca gaccatgcac tgtttcaaaa tgcgtatcct tgagatgcag cttctcggca
    17761 tagttgttca tcatttcaac aaactgccgt tgcccaccgg caatatagtc agccagagca
    17821 acacaagcgt catttccgga atccacaatt aaaccacggc ttaaatcacg taccgatacg
    17881 cgatcgccct ctttcaaaaa catcagtgaa gaaccgacaa acaccggatt atctttcgcc
    17941 cacgcatcgc gccccacggt gacaatatcg tctggcgtaa tgcgatgact atcgatagcg
    18001 cgatccacga cataacccgt catcagcttt gtcaggctgg cgggattgcg ctgttgatgc
    18061 tcattacccg cggtgaggat ctgaccggtg gtgtaatcca tcaataccca ggacccggca
    18121 tgaatctctg gaggctgagg tgaaaaagga atgttttccg ccgcaaaacc agacgataag
    18181 ttaaaaacga acaaagaagc agcaataata agacggcgtt tcaacagcaa accctcagga
    18241 gtttcaaata gctgttcttt ttacggaaat acttatgaac tggctggaat aaagtgcaag
    18301 aaaatgtgac taccctctca tttttatctg acatgatctg ttgccactcg ctgccaaatt
    18361 gtggcgctaa agctgattag cacggtgata tttgatactc tggcagacag cagaaataac
    18421 ggatttaacc taatgatgaa tgacggtaag caacaatcta cctttttgtt tcacgattac
    18481 gaaacctttg gcacgcaccc cgcgttagat cgccctgcac agttcgcagc cattcgcacc
    18541 gataacgaat tcaatgtcat cggcgaaccc gaagtctttt actgcaagcc cgctgatgac
    18601 tatttacccc agccaggagc cgtattaatt accggtatta ccccgcagga agcacgggcg
    18661 aaaggagaaa acgaagccgc gtttgccgcc cgtattcact cgctttttac cgtaccgaag
    18721 acctgtattc tgggctacaa caatgtgcgt ttcgacgacg aagtcacacg caacgttttt
    18781 tatcgtaatt tctacgatcc ttacgcctgg agctggcagc atgataactc gcgctgggat
    18841 ttgctggatg ttatgcgtgc ctgttatgcc ctgcgcccgg aaggaataaa ctggcctgaa
    18901 aatgatgacg gtctaccgag ctttcgcctt gagcatttaa ccaaagcgaa tggaattgaa
    18961 catagcaacg cccacgatgc gatggctgat gtgtgcgcca ctattgcgat ggcgaaactg
    19021 gtaaaaacgc gtcagccacg tctgtttgat tatctcttta cccatcgtaa taaacacaaa
    19081 ctgatggcgt tgattgatgt tccgcagatg aaacccctgg ttcacgtttc cggaatgttt
    19141 ggggcatggc gcggcaatac cagctgggtg gcaccgctgg cgtggcatcc agaaaatcgc
    19201 aatgccgtaa ttatggtgga tttggcagga gacatttcgc cattactgga actggatagc
    19261 gacacattgc gcgagcgttt atatactgca aaagccgatc ttggcgataa cgccgccgtt
    19321 ccggttaagc tggtgcatat caataaatgt ccggtgctgg cccaggcgaa tacgctacgc
    19381 ccggaagatg ccgaccgact gggaattaat cgtcagcatt gcctcgataa cctgaaaatt
    19441 ctgcgtgaaa atccgcaagt gcgcgaaaaa gtggtggcga tattcgcgga agccgaaccg
    19501 tttacgcctt cagataacgt ggatgcacag ctttataacg gctttttcag tgacgcagat
    19561 cgtgcagcaa tgaaaattgt gctggaaacc gagccgcgta atttaccggc actggatatc
    19621 acttttgtcg ataaacggat tgaaaagctg ttgttcaatt atcgggcacg caacttcccg
    19681 gggacgctgg attatgccga gcagcaacgc tggctggagc accgccgcca ggtcttcacg
    19741 cccgagtttt tacaaggtta tgcagatgaa ttgcagatgc tggcacaaca atatgccgat
    19801 aacaaagaga aagtggcgct gttaaaagcg ctttggcagt acgcggaaga gatcgtctaa
    19861 tttgaagcct ccgccgctgg tacggcggag gtcaatgttg agtcaggctt tttgaacggt
    19921 gatactccac gctgcatcgc caatctgctg ataatcggtg atggcatgcc cttcttctgc
    19981 cgcccactgt gggattgctt cagttgcctg ggtgcaatca aactcaatta ccaactcatc
    20041 gccgctggcc atttctgcca gtgctgcttt tgcttctatg agtggaaacg gacagacctg
    20101 cgtcaccaca tccagctttt taatcgccat ctttacacct tacttaattt gccgcagcag
    20161 ttgccagtcg cgccttacgc tgcggtcgaa catacacaag ccaggacgca gtccagactc
    20221 cgagaatcat aaatacaagg ccaatccagc cctgccaggt catcattgca gtcataacca
    20281 gtccattacc gataaagcaa ccacctgcaa tactggcacc gaagcccatc agtacaccac
    20341 ctaacccgct acgtaatgtt gtttgtgcat cagctgcgcg aacgcggaac tcacggctcg
    20401 ctttggctgc aataaatgac cccacgaaga tccctaacac caggaaaacg ccccagttaa
    20461 tgtatttcac gtcacccgcg accagaaatt gcagaatatt ggccgttggg gaagtgattc
    20521 ctaacccaaa catgcgtccg gttgcttcac tcaggggcca ggctaaaagc gcaatcaaac
    20581 cgatgagtac agcagtgaca aagggatgcc agcgtttttc aaacagaata tgagcgatcc
    20641 cagttcggcg cggcggtaag gtcgcgactt tgagttttgg cttcttcaac tctttcatca
    20701 ccacccagag cgttatcacc agcaaaacgg caaccaacgg ccaaacagac aaattaaaag
    20761 tctcagcaat agagttatgt tcagtactgt agtgctgcaa ggtttgattt aaaccactgg
    20821 catgtggaga acgcatcacc gcactcatca ccatataagt gaaaagcgcg atccaactgc
    20881 cgatcaatcc ctcacccgcg cgataccagg tcccggtggc acatccaccc gccagaacaa
    20941 ttcccagccc gaagatatac ccacctataa cagtaccaag ccacgggaac gcctcggctt
    21001 cgtaagtcag taggcccgcc tgaattaacg caaaaacccc cacgctttgt accgaaattg
    21061 caatcagcaa ggcgtaaaac atgcgattat ttttcgcgat atacatatcg cgaaaaccac
    21121 ctgtcaggca aaaacgccca cgctgcatga caaatcccag cagagcacca caaattagcc
    21181 cgcttaatat cattgaaaac ataaattaat taaccagatg aatgttaatg aggaaattat
    21241 tcatgactgg tggaatgcag accaataacc aaattctcta aattagaaca aatggttatt
    21301 aatgaggatt tcgataaaaa aaaataaaaa aaccggagcc aaatattcat tcgactccgg
    21361 ttgattatta gaaaagatga ttatgcaacg tcttcgtact gcggaactgg gttacggaag
    21421 ctcttggtga cgcaagccag gtaaatcaga ccgatagcag cccaaatcag acccagaacc
    21481 attgagcttt cttccaggtt aacccacagc gcaccaacgg tcagcgcacc acacatcggc
    21541 aggaacagat actggaagtg atctttcagc gtcttattac gcttctcacg gatccagaac
    21601 tgcgagatga ccgacaggtt aacgaaggtg aacgccacca acgcaccaaa gttaatcagc
    21661 gctgtagcca ttaccaggtc gaagttgatt gccagcaggg caatcgcgcc aaccaggatg
    21721 atgttcatcg ccggagtacg ccatttcggg tgtacataac cgaagaagct tttcgggaat
    21781 acgccgtcac gacccattac gtacatcaga cgcgctacgc ctgcatgcgc cgccataccg
    21841 gacgccagta cggtaatggt ggagaagatc agcgcgccaa cctggaacgc tttacccgca
    21901 acgtacagca tgatttcagg ctgtgacgca tccggatctt taaagcgaga gatatccggg
    21961 aagtacagct gcaggaagta agttgcaaag atgaagatca tgccgccaat cagcgcggtc
    22021 aggaaaatcg cacgcgggat cacgcgctct gcatctttgg tttcttccga caggttgctg
    22081 atgccgtcaa agccggtaaa ggaaaagcac aggattgtcg ccccggtaat catcggaata
    22141 acatgcgcat cgccagacca gaacggacga gtgctcgcca gcgtaccagc gccttcacct
    22201 tcaaatacgc cataaacaac catgcccaga atcaccgcga tcagcactac ctgcaacacg
    22261 acgattacag tgttgaagtt cgctacggat ttcagactac gcaggttaaa ggcggtcata
    22321 aaggccacca gcgccaccac aaacatccac gatgggatgg aaggcaccag agcttcaaaa
    22381 tagattttcg ccagcagaat gttgatcatc ggcgcgaaca gatagtcgag cagagaagac
    22441 caacccacca taaagccgac agtcgggcta atggatttct gggcgtaagt atatgcagag
    22501 ccagcagaag gatagcggcg aaccagcttc ccgtagctca gagccgtaaa caggatcgca
    22561 atcaatgcga acgcataggc tgtcggcaca tgaccatccg tgaggcctga aacgatacca
    22621 aaagtatcaa acagcgtcat cggctgcata taggcaagac ccatcattac aaccggaact
    22681 aacgtaagcg ttttacgcaa ttccacgcga gaggtgtttg gagtaacgtt atgcgacatg
    22741 gtcattctcc tttacggtga taaccgtcgc gtaagcaaaa aattgcccca tttttttgga
    22801 ttcctcagcg acaacaactg tcgattttta gtaaatatct atccggtacg aagcccggcc
    22861 tcttggtatg aatgattggt ttgaagcaaa aaataaccga cgctgatgaa acgtcggttt
    22921 ttagtcattt tttgacagcg gcgcattgtg cctaaatggg ggggaaatga caagagaatg
    22981 agaagcttgt cataataatt tttctttaaa tggctgattt tccgtcatca gattttgcat
    23041 aaacaccggc gatcgcacta tttgctaaaa tttcatcccg ccaccagccg ctggcaagcc
    23101 ctgcggtctg ttcgttccag ccaatccaga cgggttcaaa gagagtttgg gcgaccactt
    23161 tcttctcgat taacgcgcca ctatcgagaa aacgttgcac cagataacgg ggtaaatagc
    23221 cgcagccgag gccgctaatt tgcagctcca gcttggtttt aaaatcgaaa acggtaatgg
    23281 cttcttgttc gtcgagaagt tgtgaggccg tagaagcgcc tgcctgcgca gtgtccccca
    23341 ccacaatcgc ccgatatcgc ttaatgatac gacggtttaa cggctcttct tcctgggcta
    23401 acggatgatg aggcgcgacc gcaaagactt gttccagatc gcccaaccgc gagaaaccaa
    23461 actcactgga agatggtggc tcatgcatcg cgccaacaat aatatccgct cgcccctgag
    23521 tcagggcatc ccaggaacca ccgagtacgc cattgataaa cttcaggcgc gtcacgctat
    23581 gatgttgata aaaagcttca atcagtggcg caagcagaga aaaaggaaac gtgtcgtcca
    23641 cgccaatcac cagctcgttt tcccagcctt catgcagttt gatcgcctgc ttttccagtt
    23701 ctcgcacggt atgcagaact tcccgcccct tctctaataa catttttccg gtacgggtga
    23761 atttagcgcg gtgaccgcta cgatcaagta attgaatatt aaggtcgctt tccagcttgt
    23821 gaacggtgta actcagagcg gacggcgtct tgtagagttt agccgacgcc gccgcaaaac
    23881 tgccttcttt ttctaaggca tcgaggatca tcaacacatc cagcagttgt ttcatacacg
    23941 cccccttgca gtgcatgcgg cgtcctgctg gcgtgatcgt ttactccagc ggcattacca
    24001 gcggatcggg atactggtat tcaaatccca gttcattaca aatccgactg ccatcaataa
    24061 tcttaccttt gccgctgtcc agactatttc tgaactgcgg aggttccagc cccagtaaac
    24121 gggccatctg cggatagaaa acattacgcg cagggtgagc tggcgcacat atattataga
    24181 tgtgtccgcc tttaggtgcc tgtaacaaca gagtgatagc gccaataaca tcttctaaat
    24241 ggactaaatt aacaccatgt tcaccatcag gcgcggtttt tccggcaaag aagcgtccgg
    24301 gatgacgtcc cggtccgacc agccccgcaa gacgcagaat atcgaccgaa gtaccgggta
    24361 aattgtgcag ccagtcttcg agttcttcta atactcgtcc actgttggtc accggattac
    24421 gcggggtggt ttctttcacc gtgccttgcg cgtcgccata gacagacgtt gagctggtaa
    24481 aaataatgcg aggaatacga tgggccagcg cgctatccac taactcttgt accgcttgta
    24541 aatagaactc atcgccgggg ccgctacgac gtgccggaag cgtaatgacc agcgcatcgg
    24601 catccatcag ggcatccaga tcgtcagaat cgcaaactaa ctcaggctcc atgcgaagca
    24661 gatagctatc aatgccactc attcgggccg cttcgacacc atcttgtgtg gttttactcc
    24721 cggtgacttg ccagcctcgc gctgaaagtg acatcgccag cggcatgcct aaccacccta
    24781 acccgacaat tgcgaccttt ttcatccgtt atctcctgat acaccttaga tctataaggc
    24841 tacgctagcg tatcaaaact gacaattcat tctatgaatg aatctgttca ataatgataa
    24901 cgacatgctg caatgagcag tgaatcgtcg gtaaccgcgt ataccagacg gtgctcctct
    24961 gtaatgcgtc gggaccagaa acctgacaaa ttatgtttca ggggttctgg cttcccctta
    25021 ccttcaaatg gcgttctgcg ggtatctttg ataagttcat tgatcttttt aacaattcgc
    25081 ttatctgttt cctgccagta cagataatca tcccatgatt cctcagacca gattagtttc
    25141 actcaataat gtccttttct gttcctttgc ctgatttcag gctatcgatt gagtccatca
    25201 atctccgggc gttagcgggg gagcgcagta gataagccgt ctcttccagc gagttgtatt
    25261 cttcgagtga catcagaaca caagcctctc cattctgacg agtaataagg atcggggcat
    25321 gatcttcaac ggctttcatc attgttgccg acaaattctg acgcgcttcg ctgtagctaa
    25381 ttgtacgcat gtcaatctcc tcttttgtac agtttattgt acaatgatgg gcgttaatta
    25441 actatttatt aattagtttg tagatcaagg tattgtcagt gagacgaaaa tccaggcttc
    25501 gctatttttg gtgccgtcag cttagaggac agtcctctta gccccctcct ttccccgctc
    25561 attcattaaa caaatccatt gccataaaat atataaaaaa gcccttgctt tctaacgtga
    25621 aagtggttta ggttaaaaga catcagttga ataaacattc acagagactt ttatgacacg
    25681 cgttcaattt aaacaccacc atcatcacca tcatcctgac tagtctttca ggcgatgtgt
    25741 gctggaagac attcagatct tccagtggtg catgaacgca tgagaaagcc cccggaagat
    25801 caccttccgg gggctttttt attgcgcggt tgataacggt tcagacaggt ttaaagagga
    25861 ataacaaaat gacagacaac actcgtttac gcatagctat gcagaaatcc ggccgtttaa
    25921 gtgatgactc acgcgaatta ctggcgcgct gtggcattaa aattaatctt cacacccagc
    25981 gcctgatcgc gatggcagaa aacatgccga ttgatattct gcgcgtgcgt gacgacgaca
    26041 ttcccggtct ggtaatggat ggcgtggtag accttgggat tatcggcgaa aacgtgctgg
    26101 aagaagagct gcttaaccgc cgcgcccagg gtgaagatcc gcgctacttt accttgcgtc
    26161 gtctggattt cggcggctgc cgcctttcgc tggcaacgcc ggttgatgaa gcctgggacg
    26221 gcccgctctc cttaaacggt aaacgtatcg ccacctctta tcctcacctg ctcaagcgtt
    26281 atctcgacca gaaaggcatc tcttttaaat cctgcttact gaacggttct gttgaagtcg
    26341 ccccgcgtgc cggactggcg gatgcgattt gcgatctggt ttccaccggt gccacgctgg
    26401 aagcaaacgg cctgcgcgaa gtcgaagtta tctaccgctc gaaagcctgc cttatccagc
    26461 gcgatggtga aatggaagaa tccaaacagc agctgatcga caaactgctg acccgtattc
    26521 agggtgtgat ccaggcgcgc gaatcaaaat acatcatgat gcacgcaccg accgaacgtc
    26581 tggatgaagt catcgccctg ctgccaggtg ccgaacgccc aaccattctg ccgctggcgg
    26641 gtgaccaaca acgcgtagcg atgcacatgg tcagtagcga aaccctgttc tgggaaacga
    26701 tggaaaaact gaaagcgctg ggtgccagtt caattctggt cctgccgatt gagaagatga
    26761 tggagtgatc gccatgagct ttaacacaat cattgactgg aatagctgta ctgcggagca
    26821 acaacgccag ctgttaatgc gcccggcgat ctccgcttct gaaagcatta cccgcactgt
    26881 taacgatatt ctcgataacg tgaaagcacg cggtgatgac gccctgcggg aatacagcgc
    26941 gaagtttgat aaaaccacgg ttaacgcgct gaaggtgtct gctgaggaga ttgccgccgc
    27001 tagcgaacgc ctgagcgacg agctaaaaca ggcgatggcg gtggcagtaa aaaatattga
    27061 aaccttccac actgcgcaaa aactgccgcc ggtagatgta gaaacgcagc caggcgtgcg
    27121 ttgccagcag gtcacgcgcc cggtcgcttc agttgggttg tatattcctg gcggctccgc
    27181 cccgctcttc tcaacggtat taatgctggc gactccggcg cgtattgcgg gctgtaaaaa
    27241 agtggtgctg tgctcaccgc cgccgattgc cgatgagatc ctttatgcgg cgcagctgtg
    27301 cggtgtgaag gacgtgttta acgtcggcgg cgcacaggcc attgccgcgc tggcgtttgg
    27361 tacggaatct gtgccgaaag tggacaaaat cttcgggccg ggtaacgcct ttgtcaccga
    27421 agcgaaacgt caggtgagcc agcgtctgga cggtgcggct atcgatatgc ccgcaggccc
    27481 gtcggaagtg ttggtcattg ccgacagcgg cgcaacgccg gatttcgtgg cttctgattt
    27541 gctttctcag gctgaacacg gcccggactc acaggtgatt ttactgacgc ctgacgctga
    27601 tatggcgcat caagttgccg aagccgtcga acgccagtta gcagaactgc cgcgtgccga
    27661 aaccgcccgc caggcactga acgccagccg cctgatcgtg actaaagatt tagcgcagtg
    27721 cgtagagatc tccaaccagt acggcccgga gcacctgatc atccagaccc gcaacgcccg
    27781 cgatctggtc gatggcatca ccagcgccgg ttcggtattt cttggtgact ggtcaccgga
    27841 atcggcaggt gattacgcct ccggcaccaa ccacgttctg ccgacttacg gttacaccgc
    27901 cacctgttcc agcctcgggc tggcagattt ccagaagcgt atgaccgtgc aggaactgtc
    27961 gaaagaaggt ttctccgcgc tggcttcgac cattgaaaca ttggccgccg ccgagcgcct
    28021 gaccgcccat aaaaatgccg ttactttgcg tgttaacgcc cttaaggagc aagcatgagc
    28081 accgtgacta ttaccgattt agcgcgtgaa aacgtccgca acctgacgcc gtatcagtcg
    28141 gcgcgtcgtc tgggcggtaa tggcgacgtc tggctgaacg ccaacgaata ccccactgcc
    28201 gtggagtttc agcttactca gcaaacgctc aaccgctacc cggaatgtca gccgaaagcg
    28261 gtgattgaaa attacgcgca atatgcaggc gtaaaaccgg aacaggtgct ggtcagtcgt
    28321 ggcgcggacg aaggtattga actgctgatt cgcgcttttt gcgaaccggg taaagacgcc
    28381 atcctctact gcccgccaac gtacggcatg tacagcgtca gcgccgaaac gattggcgtc
    28441 gagtgccgca cagtgccgac gcaggacaac tggcaactgg acttacaggg catttccgac
    28501 aagctggacg gcgtaaaagt ggtctatgtt tgcagcccca acaacccgac agggcaactg
    28561 atcaatccgc aggattttcg cactctgctg gagctaaccc gcggaaaagc cattgtggtt
    28621 gccgatgaag cctatatcga gttttgcccg caggcatcgc tggctggctg gctgacggaa
    28681 tatccgcacc tggctatttt gcgcacactg tcgaaagctt ttgctctggc gggccttcgt
    28741 tgcggattta cgctggcaaa cgaagaagtc atcaacctgc tgatgaaagt gatcgccccc
    28801 tacccgctct cgacgccggt tgccgacatt gcggcccagg cgttaagccc gcagggaatc
    28861 gtcgctatgc gcgaacgagt gacgcaaatt attgcagaac gcgaatacct gattgccgca
    28921 ctgaaagaga tcccctgcgt agagcaggtt ttcgactccg caaccaacta cattctggcg
    28981 cgctttaaag cctccagcgc agtgtttaaa tctttgtggg atcagggcat tatcttacgt
    29041 gatcagaata aacaaccctc tttaagcggc tgcctgcgaa ttaccgtcgg aacccgtgaa
    29101 gaaagccagc gcgtcattga cgccttacgt gcggagcaag tttgatgagt cagaagtatc
    29161 tttttatcga tcgcgatgga accctgatta gcgaaccgcc gagtgatttt caggtggacc
    29221 gttttgacaa actcgccttt gaaccaggcg tgatcccgga actgctgaag ctgcaaaaag
    29281 cgggctacaa actggtaatg atcaccaatc aggatggtct gggaacacaa agtttcccgc
    29341 aggcggattt cgatggcccg cacaacctga tgatgcagat cttcacctcg caaggcgtac
    29401 agtttgatga agtgctgatt tgtccgcacc tgcccgccga tgagtgcgac tgccgtaagc
    29461 cgaaagtaaa actggtggaa cgttatctgg ctgagcaagc gatggatcgc gctaacagtt
    29521 atgtgattgg cgatcgcgcg accgacattc aactggcgga aaacatgggc attaatggtt
    29581 tacgctacga ccgcgaaatc ctgaactggc caatgattgg cgagcagctc actaaacgag
    29641 accgttacgc tcacgtagtg cgcaacacca aagagacaca gattgacgtt caggtgtggc
    29701 tggatcgcga aggtggcagc aagattaaca ccggtgttgg cttctttgat cacatgctgg
    29761 atcagatcgc cacccacggt ggtttccgta tggaaatcaa cgtcaaaggc gacctctata
    29821 tcgacgatca ccacaccgtc gaagataccg gcctggcgct gggcgaagcg ttaaaaatgg
    29881 ctcttggcga caaacgcggt atttgccgct ttggttttgt gctaccgatg gacgaatgcc
    29941 ttgcccgctg cgcgctggat atctctggtc gcccgcacct ggaatacaaa gccgagttta
    30001 cctaccagcg cgtgggcgat ctcagcaccg agatgatcga gcacttcttc cgttcgctct
    30061 catacaccat gggcgtgacg ctacacctga aaaccaaagg taaaaacgat caccaccgtg
    30121 tagagagcct gttcaaagcc tttggtcgca ccctgcgcca agccatccgc gtggaaggcg
    30181 acaccctgcc ctcgtcgaaa ggagtgctgt aatgaacgtg gtgatccttg ataccggctg
    30241 cgccaatctg aactcggtga agtcagccat tgcgcgccac ggttatgaac caaaagtcag
    30301 ccgtgacccg gacgtggtgt tgctggccga taaactattt ttacccggcg ttggcactgc
    30361 gcaagcagcg atggatcagg tgcgtgagcg cgagctgttt gatctcatca aagcctgtac
    30421 ccaaccggta ctgggcatct gcttagggat gcaactgctg ggtcggcgca gcgaagagag
    30481 caacggcgtc gacttgctgg gcatcattga cgaagacgtg ccgaaaatga ccgactttgg
    30541 tctgccgctg ccgcatatgg gctggaatcg cgtttacccg caggcaggca accgcctgtt
    30601 tcaggggatt gaagacggcg cgtactttta ctttgttcac agttacgcaa tgccggtcaa
    30661 tccgtggacc attgcccaat gtaattacgg cgaaccgttc accgcggcgg tacaaaaaga
    30721 taacttctac ggcgtgcagt tccacccgga gcgttctggt gccgctggcg ctaagttgct
    30781 gaaaaacttc ctggagatgt gatgattatt ccggcattag atttaatcga cggcaccgtg
    30841 gtgcgtctcc atcagggcga ttacggcaaa caacgcgatt acggtaacga cccgctgccg
    30901 cgcttacagg attacgccgc gcagggtgcc gaagtgctgc atctggtgga tctgaccggg
    30961 gcaaaagatc cggcaaaacg tcaaatcccg ctgattaaaa ccctggtcgc gggcgttaac
    31021 gttccggtgc aggttggcgg cggtgtgcgt agcgaagaag acgtggcggc gttactggaa
    31081 gcgggcgttg cgcgcgtggt ggttggctcc accgcggtga aatcaccaga aagggtgaaa
    31141 ggctggtttg aacgtttcgg tgccgatgcc ttagtgctgg cgctggatgt ccgtattgac
    31201 gagcaaggca acaagcaggt ggcggtcagc ggctggcaag agaactcggg cgtttcactg
    31261 gaacaactgg tggaaaccta tctgcctgtc ggcctgaaac atgtgctgtg taccgatatc
    31321 tcgcgcgacg gcacgctggc aggctctaac gtctctttat atgaagaagt gtgcgccaga
    31381 tacccgcagg tggcatttca gtcctccggc ggtattggcg acattaacga tgttgctgcc
    31441 ctgcgaggca ctggcgtgcg cggcgtaata gttggtcgtg cattactgga aggtaaattc
    31501 accgtgaagg aggccatcgc atgctggcaa aacgcataat cccatgtctc gacgttcgtg
    31561 atggtcaggt ggtgaaaggc gtacagtttc gcaaccatga aattattggc gatattgtac
    31621 cgctggcaaa acgctacgcc gaagaaggtg cagacgaact ggtgttctac gatatcaccg
    31681 cttccagcga tggccgtgtg gtagataaaa gctgggtatc tcgcgtggcg gaagtgatcg
    31741 acattccgtt ctgcgtggcg ggtgggatta agtctctgga agatgccgcg aaaattcttt
    31801 cctttggcgc ggataaaatt tccatcaact cccctgcgct ggcagaccca accttaatta
    31861 cccgcctggc ggatcgcttt ggcgtgcaat gcattgtggt cggcattgat acctggtacg
    31921 acggcgaaac cggtaaatat catgtgaatc aatataccgg cgatgaaagc cgcacccgcg
    31981 tcactcagtg ggaaacactc gactgggtac aggaagtgca aaaacgtgga gccggagaaa
    32041 tcgtcctcaa catgatgaat caggacggcg tgcgtaacgg ttacgatctc gaacaactga
    32101 aaaaagtgcg tgaagtttgc cacgtcccgc taattgcctc cggtggcgcg ggcaccatgg
    32161 aacacttcct cgaagccttc cgcgatgccg acgttgacgg cgcgctggct gcttccgtat
    32221 tccacaaaca aataatcaat attggtgaat taaaagcgta cctggcaaca cagggcgtgg
    32281 agatcaggat atgttaacag aacaacaacg ccgcgaactg gactgggaaa aaaccgacgg
    32341 actaatgccg gtaattgtgc aacacgcggt atccggcgaa gtgttaatgc tgggctatat
    32401 gaacccggaa gccttagaca aaaccatcga aagcggcaaa gtcaccttct tctcgcgcac
    32461 taaacagcga ctgtggacca aaggcgaaac gtcgggcaat ttcctcaacg tagtaaatat
    32521 tgcgccggac tgcgacaacg acacgctact ggtactggcg aatcctatcg gaccaacctg
    32581 ccacaaaggc accagcagct gcttcggcga caccgctcac caatggctgt tcctgtatca
    32641 actggaacaa ctgctcgccg aacgcaaatc tgccgacccg gaaacctcct ataccgccaa
    32701 actgtatgcc agcggcacca aacgcattgc gcagaaagtg ggcgaagaag gcgtagaaac
    32761 cgcactggca gcgacggtac atgaccgctt tgagctgacc aacgaggcat cggacttgat
    32821 gtatcacctg ctggtgctgt tgcaggatca ggatctggat ttaacgacgg taattgagaa
    32881 cctgcgtaaa cggcatcagt gagttgcagg gtaagcggat gcgatgttgt tgtcgcatcc
    32941 ggcaaaaaaa aagggcaagg tgtcaccacc ctgcccgttt tctttaaagc tgcaaagatt
    33001 acttcgcgtt ataattacgc agagcgttac gccccagcac aatccccgcg ccaaccatgc
    33061 cgcccagcag caccgccaga atcaaggtaa ttgccttttt cgggctatcg cgacgaatag
    33121 gtaacgtcgg tttcatcaca taacggtaag catgaatatc aagatcatca acctttaagt
    33181 tttcaatatc caacaggttt tgacgtgtct gatagtagtt tggtgagaac accaacggac
    33241 gggtcgcctc atgcttaatc atcgactcca gcgcttcgct ccccaaaagg aacaacgtat
    33301 cttgtgtcac atcttcagtc tgctgaacct gtggctttgc cacctgcgcc tgattcgcat
    33361 actgcaacgc ttcctgaatc tgacggatac gcagatcttt ctgctcctgc gcgactactt
    33421 cctgcgttct taaagagtcc tgcaagtttt tccgtcccag agcaatgttg tctttgagat
    33481 ccttttctag ctcttgattc actttatcat caacttgctg aatgtattgg gccaacttca
    33541 tttgtgctcc ctctgcagtt tgcccaacat aagaaacagt caatggtaat tgctggttct
    33601 taacagaagg ttcgatggta agtttttctg gttcttcctg attatccagc gtttctgcta
    33661 atgcagagaa ggcagaactg aagcgaccaa ttaacgtctc ctgtaaatcc gatactttcg
    33721 gtgcagcctg accatagata acattcatgg cattgttata gccagcaatt tgccccacgt
    33781 cgggctgagt gataattgct gttgacgtcc atttctcctt cgctactgcc aaatatccaa
    33841 tagccagggc aatagccaca atgacggaaa tgatgattgt catcttgcca cgccacaact
    33901 gcactagtaa atcaatcaaa tcaatctgtt ccgggtcatg gttttgccca gaaacattat
    33961 tattttctac tctcatcatt accctaactg acggaaattt ccaagtccgc atagtttagc
    34021 gataatccta gtgaatgcta taggataagt gataaaaata atgaattatt ggggaggtaa
    34081 caaaagacaa tcagggcgta aatagccctg ataacaagat gttagtcgct gccaaagaga
    34141 tcgcgggtgt ataccttatc tgccacatct ttaagctctt ctgccattcg gttagagata
    34201 atgacgtcgg cttgttgttt gaagatggcg agatcacgtt ccagccgaga gttgaagaat
    34261 gagtcttctt tcatcactgg ctcgtagatg atcacttcaa cgcctttcgc cttgatacgc
    34321 ttcataatcc cctgaataga agacgcacgg aagttatctg aaccgctctt cataatcaga
    34381 cgataaatac ccaccacttg cggtttgcgt gacaaaatgg catcggcaat aaaatcctta
    34441 cgcgtgcggt ttgcatcgac aattgccgag atcaggttat tcggcacaga ctgatagttc
    34501 gccagtaact gcttggtatc tttcggcaag caataaccac cataaccaaa cgacgggttg
    34561 ttgtaatggt tgccaatacg cggatcgaga caaacgcctt cgataatctg gcgagtattc
    34621 agacctaaac tttctgcgta gctatccagt tcgttaaagt atgccacgcg catcgctagg
    34681 taggtgtttg caaaaagttt aatcgcttct gcttcggtcg aatcggtgaa cagggtcggg
    34741 atattttgct taatcgcgcc ttcctgtaac aacgcagcaa aacgttctgc gcgttctgaa
    34801 cgctcaccga tgacaatacg tgaaggatgg agattatcgt aaagggcttt accttcacgg
    34861 agaaattccg gagagaaaat aatattttca gtacgatatt tcttatgcat cgcttcggta
    34921 aaaccaacgg gaaccgttga tttgatgacc ataaccgcat aaggatttat ctcaactacg
    34981 tctttaatta ctgattctac actggatgta ttgaaataat tagttttagg atcatagtcg
    35041 gttggagtgg cgatgatgac ataatcagca tcccggtaag cttcattttt atctaatgtg
    35101 gcattaaagt gtattttatc tgattgcaaa aactgctgaa tttccttatc aacaatagga
    35161 gatatccgat cattcagcat agcaacgcgt gacggtaaaa tatctaatgc cacaacctca
    35221 tgattttgtg cgattagaag cccgtttgac aagcctacat agccagtacc ggaaatggtg
    35281 attttcattt catgctctca gaattaactt aactgtgaat catgatgttt ttagcatcct
    35341 gataagagct aaatgtttta acgctacgtt attagctggt caacgctacg aaaaacaatt
    35401 cagattttct ttataagaat agctgatatt tattaagtta atattaagca gtgaaattta
    35461 gtctataaga taaatggcaa aaaaaaagcc cggtgcaata tacgccgggc ctcaatttta
    35521 ctgttggtta aatcagatta atccagccat tcagtatgga acacaccttc tttatcaatg
    35581 cgcttataag tatgtgcacc gaaatagtca cgctgtgcct ggatcaggtt cgcaggcaga
    35641 acagcggcac ggtagctatc gtaataggca accgcagcgg cgaaggtcgg aaccgggata
    35701 ccgttctgta ctgcataagc aacgacatca cgcagcgcct gctggtagtc atcggcaatt
    35761 tgcttgaagt acggagccag cagcaggtta gcgatctgcg gattttcggc atatgcatcg
    35821 gtgattttct gcaggaactg cgcacggatg atgcagccag cacggaaaat cttcgcgatt
    35881 tcgccgtagt tcagatccca gttgtattct tcagacgcag cacgcagctg agagaagccc
    35941 tgagcgtaag aaacgatttt gcccagatac agcgcacggc gaactttttc gatgaactca
    36001 gccttgtcgc ctgctgactg agcttgcgga ccagagagaa ctttagatgc ggcaacgcgc
    36061 tgatctttca gagaagagat ataacgtgca aacacagact cggtaatcag cgacagcggt
    36121 tctccgagat ccagcgcgct ctggctggtc catttaccgg tacctttgtt agccgcttca
    36181 tccaggatca catcaaccag gtagttaccg tcttcatctt ttttagtgaa gatatctttg
    36241 gtgatgtcga tcaggtagct gctcagttca ccgttattcc actcggtaaa ggtctgtgcc
    36301 agttcttcgt tagagagatt caggccgcct ttcaggagag aataggcttc agcaatcagt
    36361 tgcatatcac cgtattcaat accgttgtga accatcttca cataatggcc tgcgccatca
    36421 gcaccgatat aggtaacgca cggttcgcca tcttcagcaa cggcggcgat tttggtcagg
    36481 atcggcgcaa ccagttcata ggcttctttc tggccaccag gcatgatgga cggccctttc
    36541 agcgccccct cttctccgcc ggaaacaccg gtaccgatga agttaaagcc ttcggcagaa
    36601 agctcacggt tacgacgaat ggtgtcctgg aagaaggtgt taccaccatc gatgatgatg
    36661 tcgcctttat cgagatatgg tttgagcgaa tcaatagcag catccgtgcc tgcacctgct
    36721 ttcaccatta acaggatgcg acgaggcgtt tccagagatt caacaaactc tttcaccgta
    36781 tagtaaggaa ccagtttctt gcctggattt tcggcaatca cttcttccgt cttttcacgg
    36841 gaacggttga aaatagagac ggtataacca cggctttcga tgttgagcgc aaggttgcgc
    36901 cccatcactg ccataccgac tacgccgatc tgttgctttg acattgttta ctcctgtcag
    36961 ggggtggtca ccgccgagtg tgcggcttaa atgtggtata gatgttaact cactcataga
    37021 gatggtgaat agcattacct ttgctatacc acactgaata tctcaccttt tccgtctttt
    37081 aaaacttatt tatatgaaat tactgaatta cttgttcagt aactcaagaa taagttttgt
    37141 cttttcttcc atcagcggca catcaccgcg agattccaca ttcaaccgca ccaccggttc
    37201 ggtgttggag gtgcgcaggt taaagcgcca gtcggcaaag gtcatgctga tgccatcggt
    37261 gcgatccacc gccagcgcct cgcggctaaa atgctgttcc acgcggttaa tcgcctcaaa
    37321 gggttgcgcc agtttgctgt tgatctcacc gcttgccgga aacgctgcca tccggtcgcg
    37381 caccagttcg cccagcgttt ttcctttcag gcacaccagt tcggcgacca gcagccacgg
    37441 gatcatgccg ctgtcgcagt aagcgaaatc acggaagtaa tggtgggcgc tcatttcacc
    37501 accgtagatg gcgtcttcct tgcgcatccg ttctttaata aaggcgtgtc cggttttcga
    37561 catcaccggc gtgccgcctg cggcagtcac cacatcaacg gtgttccagg agagacgcgg
    37621 atcgtggatg atcttcgcgc cgggattttt ttcgaggaat gcttctgcca gcaggccgac
    37681 aatgtagtag ccctcaataa actgcccttt ttcgtcaaac aggaagcagc ggtcaaaatc
    37741 gccatcaaaa gcaatgccca tatccgcacc gtgtttgatg accgcattgc gggtgtcgtc
    37801 gcggcattcc ggcagtagcg ggttaggaat accgttgggg aaattgccgt ccggcgtgtt
    37861 gtgcactttg attaattcca cgggtgcgcc aagggcttta aagcgggctt cgatggcgtc
    37921 caccaccgga cccgctgcgc cattcccgga gttaatcacc agcttgagcg gcgtgaggtt
    37981 tttgacattg atataaccga acaggtgatc aacgtaagcg tcacgcagat tgatttgctg
    38041 atagcgaccg cgtttggttt catcaacggg aggaaagtcg ttggcttctg ccagacgctg
    38101 gacatcgcgc agtccggtat caccgctgat cgggcgagcc ccttcgcgca ccagtttcat
    38161 gccgttgtaa tccatcgggt tatggctggc ggtaacttca atgccgccat ccacgccgag
    38221 atggaacgtg gcgaaataga tctcttcggt gccggacatg ccaatatcca gcacatcgac
    38281 gcccgcatcc tgtaaacctc tcgccagcgc cagttttaag gtttcgctgg tgaggcggac
    38341 atcaccgcct aacacaatgg ttttcggttt gagaaattcg ccataggcgc gaccaatgcg
    38401 ccaggcgata tcttcattca gttcttcgcc taatttcccg cgaatatcat aggctttaaa
    38461 gcaggttaat tttttcatgt cgttaccctt tttcaggcaa tagttggccc tgaccgaaac
    38521 ggccaatttt gtttttgtcg ttatttacat ctcggtttcg cattcattgc ctgatgcgac
    38581 gtttacaccc gtccgtagcg atccgcgaaa cgcaccacat catcctcttc gagataagag
    38641 ccggagcgca cttcaattaa atcgagcgga attttccccg ggttttccag gcaatgcatc
    38701 gcccccagcg gaatataaat ggactcgttt tcaccaagca gtttgatatc accatcaatg
    38761 gtgacttttg ccgttcccgc gacaaccacc cagtgttccg cgcggtgatg gtgcatctgt
    38821 accgacaagc cctcgcccgg tttcacggtg atgcgtttca cctggtagcg gtcgcccgcg
    38881 tcgatagagt catatttgcc ccacgggcga tagacttcgc gatgcacccg atgctcatgg
    38941 cgaccatcgg ctttgatctg ctcgaccact tttttcacat cctgcaccgc gttacggtcg
    39001 gcaatcagca ccgcatcttt ggtctgcacc actaccagat ctttcacccc gacggtggtt
    39061 acgaggccag attcggcgta cacatagctg ttttcagttt tgtgattaat cacatcgccg
    39121 tggcaaacgt tgccctcggc ggtgtgggcg ctaatttccc ataatgagga ccaggagccg
    39181 acatcgctcc agcccgcatc catcggcacc acaacggcat ctgccgtacg ttccatgacc
    39241 gcgtaatcca ccgactcttc cgggcaggcg agaaacgctt cttcatccac acgaataaaa
    39301 tcgagatccg gatcgacggc gctcatcgct ttttcacagg catcgagaat atccggacga
    39361 tattttttca gttcttcgag atagcgtccg gcgcggaaca ggaacatgcc gctgttccag
    39421 taatattcgc cgcttgccac ataggcctgg gcggtttcca gattcggttt ttcgacaaac
    39481 tgcgccactt caaaggccac cgtatcctgc tcacccgcag acacttcacc gcgacgaata
    39541 tagccataac cggtttctgg tagatccggc acaatgccga aggtcaccag cttgcccgct
    39601 tcggcatatg gcatggcatt acgcacggcg gcacggaacg cgtcttcatc ggcaatcaca
    39661 tgatccgccg ccaataccag cattaacggg tcgctctccg ggctatgacg ttttgccgcc
    39721 agcgccgcca gcgcaatggc aggtgccgtg tttcgccctg ccggttcgag aataatgttc
    39781 tcggtaagtt tgttcagttg acgcagctgt tccgcgacaa taaagcggtg ctgctcattg
    39841 caaatcacca ccgggctttc gcactccacg ccgttcaggc ggcagatggt ggtttgcagc
    39901 atggtgagat cgcctttcag gcataaaaac tgcttggggt aaagtacgcg ggaaagcggc
    39961 cataagcggc taccggagcc acctgccatc acaactggat agagtttcga ctgcgccata
    40021 attatccccg aatatcattt ataaattgac gtaacacgtt ctctttatcg agtgtgcgtt
    40081 cggcatattc acgtgccacc gtgttgtgtt tgggcagcag gagcgcctga cggatccccg
    40141 ccaccagcgc ctcgaccgat tccggttcta cgcaaaccgc aatgcccgga aaggtttcgc
    40201 aaagctgccc cagttctgtg tgggcctctg cggtaatcac cgcgttaccg cctaccgcca
    40261 gaatattggt cagtttcgac ggcaatacgg catctgccgc gccgcgtttt tgcaccacca
    40321 gatggcaatc gcccatcttc agcagtgcgg gtaaagcgtc atacgattgc agcgggaaaa
    40381 attgcatgtt gcgcagtcca cgctgctgcg ccattttttc cagccgcgct ttgccgccgc
    40441 cctgcccgac aatggcaaaa atcagcggtt catcgcgcag acgatcggca gcttcaataa
    40501 cgttttccag cccctgcttt tcaccaatat tgccggagta aagaatgatt tttttgttat
    40561 ccggcaggtc aagctggtta cgaagggcgt caacatcggc atctgcaaca tgctgaaaac
    40621 gggcaatttc cgaccagttg gggaagaaga tgacgttttc cgccgccacg cctttttcga
    40681 tggctttatt catcatcgaa cgcgaaatcg tggagacgtt atcgacgtta tgcagtccgc
    40741 tacgttcgaa cgccgttgcc agctgtgcca ctttgctgcc tttgcctttt ccggcaaggc
    40801 ccagccccag catggcgtcc acttcgtaat cctgaatatg cagcacggta cgcgcaccag
    40861 aaagtttcgc cagcaggcgc attcccggcg tgcaaaacag cgttggcacc acgccgataa
    40921 tgcgatccgg cttccagcga cgttgcgcca tcagaggaaa gaaactgctg acggcaaaac
    40981 tgccgagatg taacaggcgt ttcagggtgc tcggctgttt tggcacgtac agcgggcagc
    41041 gccacaccgt ggcggccccc tcttctcgtt tgtaccgcca ggcggaatag ttctcgccca
    41101 cctgccactg cgggtagtaa ggcggtgcgg taataacccg cacctcatga ccttgtgccg
    41161 ccagccattc caccatctcg ccggtgtatt tgccgatacc ggttaactcc ggcgagtagt
    41221 taatgccgta gaccagtatt ttcataatcc gggtactccg gcacgcttct cagcgagaaa
    41281 ataggcgcgg ctgttagcgt gaacatcgtt actggcgagc agcgcgtccg gcgtcagcca
    41341 gcggtaatcg tcatgctgct catccggcag tatcagctct tcttcagcga ctctgaagcg
    41401 aaaaccgagc accacatagt gagtggtgaa atccgtgccg gagaagttat cgtcataaaa
    41461 gtgctgccag acaccgtaaa actggcctgc tgttatcggc agacgcagcc ccagttccgc
    41521 catcgtcagc cgctcgaatg cggcttccag cgtttcgtct ttctgcacgc gccctcccgg
    41581 cacaaaccag taaccctgcg ccgggcggtt ggttcttttg ccaagcagaa actcaccgcg
    41641 actgttctcg acaataaagt cgagagagac aagcggggtg gagcgcacta ccgtggcaaa
    41701 gtcttcctga cgtaaaaaca tcattacccc cgaaagcggt cttgattctc aaggaaccac
    41761 tggtaagtgc tggcaagccc cgcttccagt gagatttcgt gataccagcc aagctgatgc
    41821 aggcgcgtca catccagcag tttgcgcggc gtaccatccg gtttgctggc atcaaaaacc
    41881 acccgacctt tgtaacccac cactttggcg atggtttgcg ccagctcgcg gatagtgcag
    41941 tcaacgcccg tgccgacgtt aatgtgcgac agcatcggct gggtgttctc cagccagact
    42001 tcgtgcgcca gctccatgac atgaatgctc gccgccgcca tatcatcgac gtgcaggaat
    42061 tcacgcatcg gtgtaccgct gccccatacc accacgtccg gtgcattctg tgccgtcgcc
    42121 tcgtggaagc gacgcagcaa tgcagggatc acatgcgaat tactcgggtg gaagttgtca
    42181 tgcgggccat acaggttggt cggcatgact gagcggtaat cgcgtccgta ctggcggttg
    42241 tatgattcgc acagtttgat cccggcgatt ttggcaatgg cgtaaggctc gttggtcggc
    42301 tccagcgtgc cctgcaataa ctcgctttct gccatcggct gttttgccag tttcgggtag
    42361 atacaggacg atccaagaaa cagcagtttg ttcacgtcgt tctgatgcgc ggcgtgaatg
    42421 atgttgctct caatcatcat gttctggtag atgaaatccg ccggataggt gttgttggca
    42481 acaatgccgc ccactttcgc cgccgccaga tagacctggt caatgcgttc gctggcaaag
    42541 aaatcatgca ccgcgcggct gtccagcagg ttcagctcgt cgcgggtgcg taataccagt
    42601 tccacatcac cgcgctgttc gagctgccgc ctgatggcag aaccgaccat cccacgatgg
    42661 ccagcgataa aaatgcgttg tttactcatg cttatgactc cagcgcgatc gccacgtcgt
    42721 agccgtgaga tttcagcaga gagtgttttt tcgccgcttc gaggtcatta gccaccattt
    42781 cagacaccat ctctctgagg gtgatttccg gtttccagcc cagtttttcg tgcgctttgg
    42841 tcgggtcgcc gagcagcgtt tcaacttcag ccggacggaa gtaacgcgga tcaacggcga
    42901 taatcacatc acccggttta acgcccggcg cgtcatgccc ggtgacggaa accacaatgc
    42961 ccttctcttc aacacccgtg ccttcaaagc gcagtttgat gcccagctgt gccgctgcca
    43021 tttccacgaa ctgacgcacg gagtactgaa cgccggtcgc gataacgaaa tcttccggct
    43081 gttcctgttg cagcatcatc cactgcattt ttacgtagtc tttggcgtgg ccccagtcac
    43141 gcagggaatc catattgccg aggtacaggc acgactccag cccctgggcg atgttggcga
    43201 ttgcgcgggt gattttgcgg gtaacgaagg tttcgccgcg gcgcggggat tcatggttga
    43261 agagaattcc gttacaagcg tacatgccgt aggattcgcg gtagttaacg gtgatccagt
    43321 aggcgtacag tttggcgacc gcatacggag atcgcgggta gaacggcgtg gtttctttct
    43381 gcggaatttc ctgcaccaga ccatacagtt cagaggtgga agcctgatag aaacgagttt
    43441 tcttttccag accgaggaag cggatcgcct ccagcaggcg cagcgtaccc atcgcgtcga
    43501 cgtcagcggt atattctggt gactcaaaag agaccgcaac gtggctcatt gcgcccaggt
    43561 tgtacacttc atccggctgt acttcacgca aaatgcgcgt caggttagag gtatcactca
    43621 ggtcgccata atgcagatgg aatttcgggt tgcaggtgtg cggatcctga taaatgtgat
    43681 ccacgcgctc ggtgttgaat gacgatgcgc gacgcttaat accatgcacc tcgtaacctt
    43741 tttccagcag aaactctgcc aggtaagaac cgtcttgtcc ggttacaccg gtgatgagag
    43801 cgacttttga catgtcttat tcctctgtat ttttgaattt attcagtttc aacgcgttcg
    43861 cgtatcacca ctgcgggatt cccccggcaa accacatttg ccggaagcga tttaaaaaca
    43921 ctgcttcgtg cacccacgac ggtgccgtcg ccgattgtga cgcctggggc aacaaagaca
    43981 tcggttgcca gccagcattt ctcgccaatc acaataggcg tggcgttaat ggtgaaatgt
    44041 tgacttgcat ggtcgtggct accggtgcat aaataacttt tttgcgatat caccgaatgt
    44101 gcgccaatgg ttatttcacc gagggtatat aaattgacgt catcgccgac ccacgcgtaa
    44161 tcacctaagg ttaatttcca cggataggta atttttactg acggacgaat aactacgttt
    44221 tttcctattt ttgctccgaa taaacgtaat aaaaaagccc gccagcgata caatacttgt
    44281 ggcgaccagg caaatattgt tgcctgtact gcccaccata attgcacttt aatagcgttg
    44341 ccgccccgga accctttcgg caccgagaaa ccgcttaaat cttgcattgg ttttccttat
    44401 attcagactt tgttatataa ggctttcgtc tttgaggtag tacgttggcg taaatgccag
    44461 gataattcag cccaaaagcc aggcacatgt aatatttgtc gttggacttt tttcgcgtca
    44521 gcacacaatt ccatattatt ggtggtagat accccaccca tggaaaattc agacaccagg
    44581 ccattgagtt ttttaaatgc ataaccggct ttatacattt tggctgccag cgcgtagtcg
    44641 gaagaaactt tatattccag gtcataacgc cattttttca agccggatac agggaaaaat
    44701 atcgcctgat gactggcggg caggctgtga taaatatacc agcccggttt ggcgctacgt
    44761 ttaattttat gcccgtcgcc aaaatccagc agcgcatcgc cggtgatcat catgttatct
    44821 ttttgcattt ttaacttacg gacaaaattt gcggcatcct gatgaaaaat atcgcccgaa
    44881 ttgagaaaca acgcgaactt gccttgtgcc atcgtaatgc ctttgttcat ggcgtcgtag
    44941 ataccgttat ctggctcgct gacaaagcgt aggttataga taccattgag attttccaga
    45001 tactcacggg tgccgtcatt ggaaccgcca tcgacaacaa tccattcgaa gctgatatct
    45061 tccgcctgcg ccagatgcgc cagcgaggca tgtgttttga ctatcccttc gaggttacga
    45121 aacgcgacag tgattatgct aagcaacatg ttcttattct tacctcgtaa tatttaacgc
    45181 ttttcgcaaa ataaacggac agacgattaa aaatgcatat tccgggctaa atatcgaacc
    45241 ggtaaaaaac agcgataccg gagtaaaaag atataattgc acgcgaaaat tgcggttatc
    45301 accaaaagcg ttgatcatca tttttatcac tttccccatg taccacagcg ataaaaacac
    45361 tgcgaaccag gaaaaataaa taatcagcag atacaaacca ttgtctatgg tttttccgac
    45421 atccgcaccg ttaaatattc cgaatgatgc gacgtattca taaagtgagc caaatctgac
    45481 tacaccgtca atatgggtca aagaataacc gaccatcacc agcggaccga caatacgata
    45541 atacgatgac gatccttccg tacctaaatc gcccagacgg gtggaaatat aaggaaacgc
    45601 gattaccaca ccaacccgga atacagccag agaaatcaat gccaacggta actttttctt
    45661 aatcgcctct ttattcagat attgaaatgc ccactccagc aaataaaaca ggataaaagt
    45721 cataacccct gaaaacgatc cggataatat tatccctgcg agaatcatag catcggtttt
    45781 aggcgttttg ataccaaact gtttgatgct gagccaaatt gagattaatg ccagagcgaa
    45841 aaatgccggt tcgaaataaa gtgctgtcgt gcgcttgccg ccgaatttaa tgaaattcag
    45901 tacatagctg ttgctgtaaa tcagatattt cgaaatcgac tccataatac tgctgccacc
    45961 ggtgaggata atttgcgcca tctccaccgc cgccagcgcc accaccagcc cgatcaccag
    46021 ataaaagaaa cgtaatatct tgcgatggtt gtgtggcgaa atcgttttaa agcgaatact
    46081 ccacaccatg ccaataatga tcacaatata gacaaacagc atggttgaag tgacgtattt
    46141 actggcatcc agcgactgac caaacagata gttaaacgcc gtgagtcccg caccaatccc
    46201 taaggcaatc atcaattttt taacgctgat gcgttctaaa aacagcagta acaagacagg
    46261 taaaaaagtg acgatggtga tggggaaact ttcgccaagc tgggcgattt tgacgttaac
    46321 cagcaggtag atcagcggca gcagcaggta gctacagatt ctgatagaag ttgacatact
    46381 cctccagcat ctgttgtcca ctgtaggtgg cgcggctgcg ttggctgaac tcagccagcg
    46441 tggtaccaaa tatcgcctgc gcgatttccg gtttgcttaa ctgcaccagt tgcagcacgt
    46501 cttcttcgtc gacggtttta ccgccggatt tttgcaacac ttcccgtgcc gcgtcgctat
    46561 gggtggcaat caccggcacg ccaattgata acgcctcaca caaaatcagc gggtagttat
    46621 cgacgcgaga actgaatacc agcgcatcca tctgattgag cgcgctcatc aacttgcgct
    46681 tgtcagtttc aaagccgtga ttaaccacgt tgccagcggt gaacggcgag aacttaccaa
    46741 aggtatgcag ttcgattttg tcgcccagcg ccatcatctc acgcaccagt tgctggttag
    46801 tcttgccgtc gtaacgcaag tcatgcgcca ccaccgcgat tttcggcttg ccctgggttt
    46861 cgcgcaccgg aggcaagtcc gccagaatcg cttcggttgc catatcaata ccattattga
    46921 taatccggca acgccctgga ccgtacaggc tattgaaagc gtcagccaca tgctggctgg
    46981 gggaaataaa ctgacagccc agcgccagca tctcacggaa taactggcgt ttgcccgcca
    47041 ccagctggtg tgcgcgatca atcttcaccg gcggatagtt atttaaggtc gggcatttct
    47101 ggcagcccgt tttccagcct tcgcaaccgt cggtaaaggc gcagcgtccg gtaacactcc
    47161 agtggtcgtg cagcgtccag accagagtga cgtccggttt atggtttttc actttttcgc
    47221 aaaagcgcac cacgctctta agatttagcc agtagctgtg cagcacatga aaatgcagga
    47281 ccaccggacc cggtgtgcga gtaatggtgc gatataactc attgaaattg ccaaacagat
    47341 cgcgattaaa cagacgaaac agggcaatat tcgccatcgc ggtcatccgc ggcgtatgtt
    47401 tgatgacctg cggatagttc tgatggctga cgctctcttt gccgcctttg ccataaccgt
    47461 agacaaaatg tgacgccagc ccctgttgca gcgcacgctg gtggagatct aacgccacac
    47521 ctgctgcccc gccttccgcc agtcgcacat taaattgcag aatattcatt taattacctt
    47581 cactcgcgct ttttctccca ccaccagcgc gttgtccggg acagagtcga gcaccacgct
    47641 gcccgcaccc acggtgacgt tgttaccaag cgtgatatca ccaagaataa tgacgttggc
    47701 accgagttcg acgccgttgc caatgtgtgg acatgccatg ttatcggcac cacgattgcc
    47761 gatagtgacg ccgtggcgaa tggtgaaatc atcccccgct accacgtttt tattgatcac
    47821 tacggcgtaa ccgtgatgga tagtaaagcg gcggccaatg gtcgcggcgg cctggatttc
    47881 ataaccgaaa aagcattcgg tgataatgcg atacagcacc agcagcgggg ccgcccacag
    47941 attgttgagg acgttctttt tgcgccacac cgaacaaaaa tgagcaacac gataggcaag
    48001 aaccatgcag cacgggcgta aactccagct gttggcacgc agatcttcca gcatctttag
    48061 cgcccccgaa taccatcagc cagacgtttg ccgttacgca ccgacagcag cgtcagcaaa
    48121 gtgcgccagg tcatacgttt attgcggatc tggtagaggg taaacagctg atattttttg
    48181 cttgcacggt caaatttatc tttgtgcttt cggtaaaagt ggaagtaccc ggagaatttt
    48241 ttcggtgacg aggtgatctg catttcaccg tgattgatat gcaggatctg cgtcgcttct
    48301 tccactttcc acggttcgcc gtactccacc accatccgca ggaagatgtc gtaatcctgt
    48361 gctgctttca gttcggtatc gaacaaacac tctttgaaac gccatgccca agtaaagacc
    48421 tgattgccaa tgatattgcg tttatagaac aggcggcgtg agtacggcga tttgggatac
    48481 agtggtaagc ttgccggttg ggaataaact tctccctggc atacgtagtc gttagcgtac
    48541 aaaaaggcgt gagtgaccag ttgctgttta tgggcgagga agacgctcag acggttgggt
    48601 gtccattcgt catcgtcatc gatcccggta atgtattccc cttgcgccag cataatcgcc
    48661 tggttacgta ccgcgcacgc cccgctgtta atgtcgttgt gaatgtaggt gatacgcgga
    48721 tcgttgaggg cagtgacgta ctgttgcagt tgttcccagg aggtggagca atcatccacg
    48781 atgatcatct cccagttgct gtagtcctgg cgcaggaccg attttatcgc ccgaatcgcc
    48841 agttgttgac ggttccaggt cggcatatag attgaaatca gcggattctc tttcataggt
    48901 tgttctcccg gcttatgagc tggtttgctt gccggatgcg gcgtgagcgc cttatccggc
    48961 ctacggggcg gtgcgaatgc aggcgtcgtt atttcgcatc cgacttatat tcgtattcgt
    49021 aatagccata atcctgatac gcgctggcgc ggcggaagat ggagttcaga atcacccctt
    49081 tcaccggaat accgttttgc tcaaagcggc tcagactggt ttccacttct ttcaatgtgt
    49141 tgaccgcata gcgcgccacc attaacgtgg ttccagcgtg acgcccgaca attgctgcat
    49201 cggtcactgc cagaatcggc ggcgtatcaa tcaacaccag gtcgtagttt ttactcgccc
    49261 agctcaccaa ttcggcaaag cgttcgctca tcaacagttc agaaggattt ggcggcacct
    49321 gaccgcgcgg gatcaggtca aatttggcaa tagaggtcgg tttagctgcg gtagtaatat
    49381 cgccctggcc aatcagaatt tccgacaggc cattgacgtt attggtaccc aacagctcgt
    49441 gggtgtagcc tttgcgcata tcgcagtcga tcaacaacac ccgtttattg gtctgactga
    49501 tcaccgccgc caggttggcg cagacaaagg ttttaccgat tgacgggctc acccctgtca
    49561 tcatcaacac attgttctgc gcctgcatca tcgcgaagtg cagactggta cgcagactgc
    49621 ggatggcttc aatcgccaga tcggttggat tccccaccgc cagtagctgg ctctgtttat
    49681 agcgtttaat ccctttgatg gttttgacgc tatcgcgcgc tttctgccat tccgacagcg
    49741 ggatgctggc atagacgctg ataccatgtt cttccagcac ctgcgggctt tcgatgccgc
    49801 gattaaacaa cgagcgcagc agcacaccca cgatagagag catcaggccg aggataattg
    49861 cgccgaggat aatcagtcct ttcttcggtt tcagcacgcc aggctgggtg attgccgggt
    49921 caacaatgcg cacatcgccg acggtgctgg cttcggtgat tttcagctcc tgctctttat
    49981 tcagcagttg catatagacc tgctgaccag actcgacatc gcgggtcagg cgcacaatct
    50041 cctgctgggt tttcggcatc gccgttacgc gaccgttaag tttggctttc tcttcttcca
    50101 gcgcctgacg tttctccagc agcgtgcggt acgccgggtg aactttggtg tacagcttgg
    50161 agatttccgc ctctttaaag gtcagttcgt tcaactgcgc gtcgatgttc accatcgaat
    50221 cgagcaccgc tttcgcttcc agcggcagat caacagaatc tttatcctga cggaaggcat
    50281 tcagtttgtt ttcggcaaca tcgaggcggc tacgtacttc cggtaactgt tgcgcgagga
    50341 aagcgaggct tttcgacgct tccgccgatt tgcgctcaat attttgttcc tgatagttac
    50401 gggcgatgct gttaagaatg tcgcggatct gttcgcgatc ttcaccggta taagtcaggc
    50461 tcagtacgcc tgcgtctttg ccgttctccg ttaccgtcag gctgttttgc agttggttga
    50521 tcatccccaa cgtggagtat ttggtgacgg taaactcact gcccgggcgg gcgtgaatgg
    50581 cttcaaccat cagcgtgacg ccttcttttt tcagcatctg gcccgcttgc ccacgggcgc
    50641 taaagccgcc atcactgctc agggtgtagt ttttattgtc cagcacatta agcgtaaaca
    50701 cctggcccgc catctctttc gggcggttaa aggtagtcac tttcaccgtc tcgttctggc
    50761 gtcccatcag tcgctcccag cccgccccga aaatcgggaa cgtgtttttg ctcactgcaa
    50821 tatcgaggtc gagatcgtcc accgttttac caagcaccag acgcgaacga atcaactgga
    50881 tctcggcgtc cgatgcaggc ggtttgttgg ctaatgccga accaatgtcc tgcactaacg
    50941 aattgccgct gttttgctcg atttgtacca gtgcgtcggc actataaatc ggcgttgcga
    51001 agaaggtgta aaccacggca cagagggcaa atacagcggt gatgccaatc acccaccagc
    51061 gcgcttcaat gacggtgccg accaggcgac caatatcgat ttcatcactg cccgttaccg
    51121 gagcggcatg ttgttttact ttttctgtca ttcttatacc tgctctgcgt tcaatgcctg
    51181 cgcccactgg cgggcagacc gttcaagtaa tgtgtacacc gctgcaaacg tttcccggct
    51241 tttgcgatac ggatcgggga tttcacattc gttatcccag tgaccaaaca gcatcacttt
    51301 gccgcgcatc tcaggtgcca tctcgcataa gcgttcgata tggcgctttt ccatggtcaa
    51361 aatcaggtca tagttgcgac acaggcggcg gctgatttgt cgtgcacagt ggccttccag
    51421 agacagttga tgttctgcgg cgacgctgat agcggtagga tcagcgccct taccgaccag
    51481 cgcgccgagt ccagcggact ccactttcag ttccgggtga taacgttgca gtaagcgttc
    51541 cgccgtcggg gaacggcaaa tattgccgac acagacaact aatatgttgt taaacatgac
    51601 gattaccagt tatgaatgtc gctggctgta tccgtcatgt aacggacacc gctaatagtt
    51661 ggcaacaact gattgatcag acggttccag cgggaaaccg gggcggtggt gacatacacc
    51721 acgtcataag gttgcaggcg gaattctgtc gccatcacca gcgacgtggc atcggacata
    51781 tccagctggt agatattggc aatcttgcca ttacgcccgc cctcgccttt cagcggacga
    51841 atgacaaaga tgccgctggc gttggaggtg gtcatgtcga taccttcagc attacccagg
    51901 gcttcagtca gggtcatgcc gctaaagtcc attttcaggg tgctctgttt cttcacttca
    51961 cccatcacaa atactttcag atcatcatta cgcggcacgt agagaatatc gccgggataa
    52021 agcagtcggt tctgattgag gtcgccgttt tgcatcagcg cctgcaaaga aatgcgttct
    52081 tcacgaccat tgtgtgttag cactacgttg cgccagtcag cggtgtcggt caggccacct
    52141 gcggcgttga tggcgtcgag aatagtcagt ggcacgttgg tgatcgcctg ttgaccggat
    52201 ttattcacct gaccggagat ataggccttt tgtgagcgga aggcggcgat attaacgtcc
    52261 acctgcgggt cagcgatgta cgtcgctaag cgcccggtaa tatcactgcg gatttcagcg
    52321 agcgttttcc cgactacgtg aaccttgccg atatacgggt aaaacatagt gccgtcaggc
    52381 tgtacccagt tgccggtgtc gctggagctg cggtactgac cggctggcgt ggtgagttcc
    52441 gggtgatccc agacggtgac attcagaacg tcgcccggcc cgacgcgata ctggtaattc
    52501 gcgatctcgc tttccagcgt catattgggg cgcgctacat tcgggcgtgg gcgtaattgg
    52561 tcaatcaggc gcggggtcag cggataaaca ttcaccattt tgtcgagatc gaaatcagcg
    52621 tcctgctgtt tgatgacgtc tttgcccatc gtcgacatat tgctgcccgg aagtactgtg
    52681 caaccgctta tcaaggttac tgacaccaat aatggcatca atttcatttt ggatttcatc
    52741 attgtttatt tatcactttg gcagagtaat tatcctgtgc actattaata gcaatatcgc
    52801 catgcacatt taccttgcag ttaattgaat aaaaatttaa ctggcatcag tcctaaaaaa
    52861 attgatttca tccgcaggct attgacagaa taattcagac tggtctttca ggcatccaga
    52921 cacgctaccg cccctggctt tttagccacc aacacactga tttagcttaa tttttcaaac
    52981 cctctctgca tacaatcgtt gatgagaaag ggttattgcg gaaattaact tccgaatata
    53041 aggtgacatt atggtaattg aatattggct ctccaataat gcaggaggaa gtgttacagc
    53101 taacggaata gcaggcaagg taacaattcg gcaattggct atttttaaga attatattaa
    53161 gtgtcattca atatggtttt taggagtttc tttaggttga caatatttaa tattgcgcct
    53221 ccacatgcga tatttcttaa ataatgtttt attattacca cttttaattc agggataatg
    53281 tgaggttatt accctcaaat aatatgagat aaatagtgct gcaacattgc atttttgccc
    53341 cgatttatcc atgatcgaat tgtgacattt gtcatacaac gaataggttt tgtacttact
    53401 atggaatgga ttgccgatcc gtctatctgg gccgggttaa tcacgctgat tgtgatcgaa
    53461 ctggtcctcg gcattgataa cctggtcttt attgccatcc tcgccgaaaa actaccgccg
    53521 aggcagcgcg accgcgcacg ggttaccggg ctgctgctgg cgatgttaat gcgcctgtta
    53581 ctgctggcgt caatctcctg gctggtcacc ctgactcaac cgctgttcag cttccgctcg
    53641 tttaccttta gcgcccgcga cttaatcatg ctgtttggtg gtttcttcct gttgttcaaa
    53701 gccacgatgg agctgaacga acggctggaa gggaaagaca gcaataatcc cacacaacgc
    53761 aaaggtgcga agttctgggg cgtggtgacg caaattgtgg tactggacgc cattttctca
    53821 ctcgactcag tgattaccgc cgtcgggatg gtcgaccatt tactggtcat gatggccgcc
    53881 gtggttatcg ctatcagcct gatgttgatg gccagcaagc cgttaacgca atttgttaac
    53941 agtcacccga cgatcgttat tctctgctta agcttcctgt tgatgattgg ctttagcctg
    54001 gtggcagaag gtttcggctt cgtcattccg aaaggctacc tgtacgctgc cattggtttc
    54061 tcggtgatga tcgaggcgct caatcagttg gctatcttta accggcgacg ttttctttcc
    54121 gctaaccaga cgctgcgcca gcggactacc gaagcggtaa tgcgcctgct tagcgggcaa
    54181 aaagaagatg cggaactgga cgccgaaacc gcgtccatgc tggtggatca tggtaaccag
    54241 cagatcttta atccgcagga acggcggatg attgagcggg tacttaatct taaccagcgt
    54301 accgtcagca gcattatgac gtcgcgccac gatattgagc atatcgatct caacgcgccg
    54361 gaagaagaaa tccgccagtt gctggagcga aatcagcata cgcggctggt ggttaccgac
    54421 ggtgatgacg cagaagattt gctcggtgtt gttcacgtta tcgacctgct acaacagtct
    54481 ctgcgcggcg aaccgctcaa cctgcgggtg ttgattcgcc agccgctggt gttcccggaa
    54541 accttgccgt tgttacctgc cctggagcag ttccgtaatg cccgcacgca ctttgctttt
    54601 gtggtggatg agtttggctc ggtggaaggg attgtgacat taagtgacgt cactgaaacc
    54661 attgtcggta acttaccgaa cgaagtggaa gagatcgacg cccgccatga tattcagaag
    54721 aatgcggacg gttcctggac ggcgaatggt catatgccgc tggaagatct ggtgcaatat
    54781 gtgccgctgc cgctggatga aaaacgtgaa tatcacacca ttgccgggct gttgatggaa
    54841 tatttacagc gtattccaaa acctggcgaa gaagttcagg tgggggatta tttgcttaaa
    54901 acgttgcagg tggaaagcca tcgcgtgcag aaggtgcaga ttattccgct gcataaggat
    54961 ggcgagttgg agtacgaggt gtgatggtat cggtcatttt acccaatagc cgaaaggaat
    55021 aagatgaggg aaccgcgcca gcggcgattt accgccggga gcccgggttg ccagggtggt
    55081 ggcgattgag ccaccctggc acgttcaccg gcttgagcgt tccatagtag cgaggaacat
    55141 caagtgaacg gaacagcctt cgatcttaca tattcagaat ataatgatgc cgccacgaac
    55201 gcctgatgcg acgctttacg cgtcttatca ggcctacagg actggtcacc tggtaaccta
    55261 catcttctcc agcaacttct tcacatcctt gccattgcgg gaatctttat tccgctcggc
    55321 ccagtcattc aggcgacgtt tcgcttcgtc ctgtagatgt ttgcgcagta actgatccac
    55381 ttgcaggctg taattgagtt gctgccagtt gccataaacc cgcagcggta ctggcgtttc
    55441 tttcaggaaa tcaatcagtt tactttcccc gttccagcca cccacgaccc gaatatcaaa
    55501 ctgggtatcg caggtttgat ctgccagatt caacatgcct tcccctgtca gcgccagcac
    55561 aggcgattga ccttgcatgt cgtttaacgt cacgacgcca tccttcaacg tcaaatcggt
    55621 ggtaaagcgg tcaagccgcg tcacgttatc aaagttttca gcggccttca catcaccgcc
    55681 attacgctct accgcttgct gaatcatctg ctggaagttc atcccttcca tgcgcgtgtc
    55741 ggtcatttcg acatgtgcct gtccttgcca gttgtggcgg aatgcgtcgg catctatgtc
    55801 agcaccggag aaatcaccag ccagtgacat ttttccggtc aacgaaatcg gatagttaaa
    55861 cgccttcaga atggtaccaa tctcaacgtt ttccagccgt ggctggaagt ttatccgcgg
    55921 atttattgat gtcgcgtcca gcgtgcccgg cagtgaaacc tgtccaccgt taagtttgcc
    55981 ctgcagttga gtaatttcca gcaaacccga cttgttggtc atttgcgtgg caacatctgt
    56041 aaaattcatt ccgcgccagc gcacgttact ggcctgcaac aaaatatcag ccgtaaagcc
    56101 ttgcagtccc tgataggccg gttcatcaat acgcgaagaa atgaccgggc gcggcaacgt
    56161 tgattggctc tgcccctgct gcgcggcacc gttttcacca ttcgcggttt cattaagcgg
    56221 gatgaggttg tcaagattca gttgcgggaa ttgcagtctc agctgccatt ccggtttctc
    56281 tgtcagcgtg acctgtgctt gcccgctcag cgtactgtca ttggcggtca agctgatttg
    56341 gttaaatgaa aggcgtttat gtgactcctg ccactgggcc tggaaactcc cctgcccctg
    56401 aataccttgt tttggtaaat cggcaccctg caactgccag ttaatttgtt caatagccgc
    56461 cgttaaatca tgcggataat cagaagcatc caccgtaccg ttaagggata ttgtgagatc
    56521 gcgctgatcg cgattaaccc gcccggagaa ctcaaatgag ccacgatgtt ggggatcttg
    56581 ttccatttgc aggcggatat tgcggattgt cacctgctcg tcatcttcat gctggaacac
    56641 cagcacgctg tccgccacct taagactgga tatatcaaac gaccatccgc gatcgtctga
    56701 cagatccggc aaggtattgt cgcgcggtgc aaccggagcg tcttcactgc gcaccgcttc
    56761 cgtctgcggc gtcagttgga tcactgcccc ttttagcatc acctgcttaa cgctcagttg
    56821 atgactcagt agtggtaaaa gcgccacgtc cagacgcatg ttgtcggcgc gaaccagtgg
    56881 ctggcttgcg ccctgggcgg tgagagacat tcgcccggag aggatactaa gctgcggcca
    56941 gacgtgccaa cgcagtggcc cgtcgagctg caattgataa ccgctacgtg cagcaacttg
    57001 cttgaccata tagtcgcgga aatcattcgg attcaccagc aacactaaca cagataaccc
    57061 ggccaccagc acgaccagga gtatcatcag cgtcgtcaga aatcgtctca tggtatcctc
    57121 aatgggcctg aattagtctt tatcgattcg gctggctacc gcgccctgct ggttgcgata
    57181 tttcgcatct tcacggcggt tgtaaggtcg cgccgccggg ccggaaagcg gctcaaagct
    57241 cagcgcacca attaacatgc ccggacgcag cgccagcggc agcttaccgg agttgtagaa
    57301 ctccagcaca atgcaaccag accagcccgg atcgatacgg tgcgcggtga cgtgcaccat
    57361 cagccccaga cgcgccaaag aggaacgccc gtccagccag cccaccagat cggctggcag
    57421 cgtcaccgac tccagcgtca ccgccagcgc cagctctcct gggtgaagat agaacgcctc
    57481 gccctcgtcg agaacgatct catcgctcat cacgcggtca agcgcggcgc tcacttcatc
    57541 tttgggaccg ctcagatcga taaacgctgc cgtgtgacca cggaaggtac gaaatttatt
    57601 gcccaggcgt acatccaccg tcgcgccgtt aatacgctcc actggcggac gtgggttgat
    57661 cgacaaacgg ccttcatcaa gccaggcttc aatatctcgg tcacacagac gcatggcatt
    57721 ttctcctttc gcgcatcact cccttaacgc caattacgtc aagggcatac caggttatca
    57781 ctgaacggta cacaatttat caagcttatt caaagaactg acttattttc gctttcaata
    57841 tatcgatcgc gatgcggttt ttcccgccgc gcggcacgat aatgtccgca tattgtttag
    57901 aaggctcaat gaattgcagg aacatcgggc gcacggtttt ttgatattgc gccatcacgg
    57961 aatccattga acgcccacgc tcgttaacgt cacgcttgat gcggcgcatc aggcagatat
    58021 ccagcggggt atcaacgaaa atggagaagt taagttcgtc acgcaaacgc gcatccgtca
    58081 gcaacaaaat gccttcgaga atgatgacct tcttcggctc aaccgtcacc gtttctttca
    58141 tacgcgtatg ttcaacatag ctgtaaaccg gcaggtcaat tgccgagccg cgtttcaacg
    58201 cttgtaaatg ctcaagcagc aggctgtgat ccatcgcgct gggatggtcg tagttggtct
    58261 taacgcgttc ttccatcgac agatggcttt gatctttgta atagcagtct tcgggaatta
    58321 cgccgatgtg ttcatcaccg acttgctcac gtaattcacg ataaagggta ctggcaataa
    58381 gactcttgcc ggaagccgat gcgccagcga taccgataat gacgcactga tgagactgat
    58441 cagtcatata tttagcgacc tgattaacct ggatgttagg aagggggcga cgaagcgcca
    58501 aacgcggcaa ttatagggat ttcatccgtc tgataccagt cgaatagcgt tgccgcgcgc
    58561 tcagagttaa ttgttgacaa agaattcccg gtggcaaatt acgttgatca gttttatgta
    58621 aggtaaaaaa tgttatacgc agttgcgcaa attatccgcc tttacgtcac tttatgagca
    58681 attcgcatat aaaatgtaaa acttttgtac tagcataaac acagaaacga atactggcga
    58741 cctggtcttg cggataaagc ggtaatgagc aaacaatcac agcatgtatt aattgccctg
    58801 ccccacccgc tgcttcacct ggtcagttta ggtttagtct cgtttatctt tacccttttc
    58861 tcgcttgagc tttcgcagtt tggcacccaa ctcgccccac tgtggttccc gacgtccatc
    58921 atgatggtgg cgttttatcg ccatgccggg cgcatgtggc cgggaattgc gctgagctgt
    58981 tcgctgggaa atatcgccgc atccatcctg cttttttcca ccagctcgct gaacatgacc
    59041 tggacgacca tcaatattgt tgaagccgtg gtcggggcag tgctgctacg taaattgctg
    59101 ccgtggtata accccttgca aaatctggct gactggctgc gtctggcact cggcagcgcc
    59161 attgttccgc ctctgttggg gggtgttctg gttgtcctgc tgacgcccgg agacgatcct
    59221 ctcagggcat ttttgatatg ggtactgtca gaatccatcg gcgctctggc actggtgccg
    59281 ctgggattgt tatttaaacc acactatctg ctgcgccatc gcaacccacg gttgcttttt
    59341 gagtcgctgc tcacgttagc catcacactg acgttaagct ggctttcgat gctgtatctg
    59401 ccgtggcctt ttactttcat tattgtgctg ttgatgtgga gcgccgtgcg cctgccacga
    59461 atggaagcct ttttgatctt ccttaccacg gtgatgatgg tgtcgctgat gatggccgcg
    59521 gatccctccc tgcttgctac gccgcgtacg tacctgatga gccatatgcc gtggctaccg
    59581 tttttgctga tcctgctgcc cgccaacatc atgaccatgg tgatgtatgc ctttcgtgcg
    59641 gaacgcaaac acatttccga aagcgaaacc cgctttcgga acgcgatgga atattccgct
    59701 atcggtatgg cgttagtggg caccgaggga caatggctgc aaaccaacaa agcgctctgc
    59761 cagtttctcg ggtacagtca ggaagagctg cgcggactca cctttcagca actgacctgg
    59821 ccggaggatc tcaataaaga tctccaacag gttgaaaagc tgataagcgg tgaaataaac
    59881 acctattcaa tggaaaaacg ctactacaac cgcaatggcg atgttgtctg ggcgttgctt
    59941 gccgtctcac tggtgcgcca cacggatggc acgccgctct attttatcgc tcagattgaa
    60001 gacattaacg agctaaaacg caccgaacag gtcaatcagc aactgatgga gcgcatcacg
    60061 ctggctaacg aagcgggcgg gattggcatc tgggagaggg agttgaagcc gaatgttttt
    60121 agctgggata agcggatgtt cgagctgtat gaaattcctc cacatatcaa accgaactgg
    60181 caggtgtggt acgagtgcgt gctgccggaa gatcgccagc atgccgaaaa agtgattcgt
    60241 gattcgttgc aatcacgctc gccctttaaa ctggaatttc gcattaccgt aaaagacggt
    60301 attcgccata tccgcgccct cgccaaccgg gtactgaata aagaaggcga agtcgaacgt
    60361 ctcctcggca tcaatatgga tatgaccgaa gtgaaacagc ttaacgaggc attgtttcag
    60421 gaaaaagagc gcctgcacat tacgcttgat tccatcggtg aagccgtggt ctgtattgat
    60481 atggcgatga aaattacctt tatgaatcca gtggcggaga agatgagcgg ctggacgcag
    60541 gaagaagcgt taggtgttcc gctcctgacg gtgttgcata ttacttttgg cgacaacgga
    60601 ccattaatgg agaacattta cagtgccgac acctcacgtt ccgcaatcga acaagatgtg
    60661 gtgttgcact gtcggagcgg cggcagttac gacgtgcatt acagtattac gccgttaagt
    60721 actctggacg gcagcaatat tggttcggtt ctggtgattc aggacgtcac cgaatcacgc
    60781 aaaatgctgc gccagctgag ctacagcgcc tcccatgatg cactgacgca tctcgccaac
    60841 cgcgccagtt ttgagaaaca actgcgtatc ctgctgcaaa cggtaaacag tacgcatcag
    60901 cgacatgccc tggtgtttat cgatcttgat cgctttaaag cggtgaatga cagcgccggg
    60961 catgcggcgg gcgacgcttt gctgcgcgaa ctggcgtcgt tgatgctgag tatgctgcgc
    61021 tccagtgacg tgctggcgcg gctcggcggt gatgaatttg gtctgctgct gccagactgc
    61081 aatgttgaaa gcgctcgttt tatcgctaca cgtattatca gtgctgtgaa tgactatcac
    61141 tttatatggg aaggccgggt acatcgggta ggtgccagcg ccgggattac cttgatcgat
    61201 gacaacaatc atcaggcggc agaagtgatg tcgcaggctg atatcgcctg ttatgcctcc
    61261 aaaaatggtg gacggggccg ggtgacggtt tacgaaccac agcaagctgc cgcacatagc
    61321 gaacgggcgg cgatgtcgct tgatgaacag tggcggatga ttaaagagaa tcagttgatg
    61381 atgatcgccc acggtgtcgc ttcgccgcgg atcccggaag cgcgtaattt gtggctgatt
    61441 tcgcttaagc tctggagttg cgaaggcgaa attattgatg aacaaacatt tcgtcgtagc
    61501 ttcagcgatc cggcacttag ccatgctctt gaccgacggg tattccacga atttttccag
    61561 caggccgcca aagcggttgc cagtaaaggc ataagcatcg ccctccccct ttccgttgcc
    61621 ggtttgagta gcgccacgct ggtgaatgat ctgcttgagc agctggaaaa tagccctcta
    61681 ccaccacggt tattacatct gattattccg gctgaagcga ttttagatca cgcagaaagc
    61741 gtgcaaaaac tgcagctggc gggatgtcgg atagtgctca gccaggtggg ccgcgatctg
    61801 caaatcttca actcgctgaa agcgaatatg gcagattacc tgctacttga tggtgagtta
    61861 tgcgccaacg tgcagggtaa tttgatggat gagatgctga ttacgattat tcaggggcac
    61921 gctcagcgac tcgggatgaa aaccatcgcc gggccagtcg ttttaccctt agtgatggat
    61981 acgctttctg gcatcggcgt cgatctgatt tatggtgatg tgattgccga tgcccaaccg
    62041 ctggatttgc tggtgaatag cagttatttc gcgattaact gagagagcgt ttgcccattg
    62101 cctgatgcga cgctaacgcg tcttatcatg cctacaaatc gctcattccc cagaccggat
    62161 aaggcgctcg caccgcatcc ggcgaccaac gtcatgcttc gtctggttgc cagccttccg
    62221 tataccagat atgcaacagc gcataagaac gccagggctt ccagcgctcg gcataacggc
    62281 ggatttgtgc cggtgtcatt cccggaaatc gccgtttaat cagataatca tccggcagaa
    62341 aaacatcttt cgcctgccag ccacgcaaag caaaataatt cgccgtccag cgcccgatac
    62401 ccggaaaagt ttgcagcgtt ttcatcgcct gctccacatc gcccggaatt gtcattggta
    62461 agctgccctc cagcgccgca tttgccagat gaatcagtgc ctctgcccgt ttcaacggca
    62521 tgcctaacgc ttttaatgcc tgcgggtcgg ctgctgccag ccgctgaggc gtggggaagc
    62581 agatatactc cggaaaatca tccagccgtt cgccataaag ctgtaccact ttggcagtca
    62641 attttgccgc catcgccacg ctcaccagtt ggcctaaaat cgcccgcaca ccctgctcaa
    62701 aagcatcaat acagccgggt aaacgcaatc cgggccgccc cgcgcctaac tttcccaacg
    62761 caccgttaac aatctgcggg ttacattgca gatcaagcag gcggctcatt ttcgccagac
    62821 actctgcggc aacaggttct aaacctgcac ttaaatttat gtgcagagta tggcgggcta
    62881 tatccggaat agcagtcact acgccgcgat attcgcccac cgccagacta cgggcataat
    62941 aattgtccgc gaccgtttcc acaccgctca ccgcacgggc ggcgagaaat cccaacatcc
    63001 acgaccagtc atacggcggc tgccagttca gggtatacat cgcatctcct tgttaatccg
    63061 ctttcagcat aaacgttatt cagacgttac gctttgcttt catattccgg ttgtcgcgac
    63121 agcaacattt cgctaaagtc acgccccttc ttcaccggca tggggattaa ttcgtgttta
    63181 ttggttttga ttacggtaca gcaaactgtt cagtggcggt catgcgtgac ggtaaaccgc
    63241 aattactgaa aatggaaaac gacagcacgc tgctgccttc aatgctttgc gcgccaacgc
    63301 gtgaatcggt aagcgaatgg ctgtaccgcc atcatgatgt tccggcagac gacgatgaaa
    63361 cgcaggcgct gttacgtcgg gcgattcgtt ataaccgcga agaagatatc gatgttacgg
    63421 cgaaaagcgt gcagttcggt ctttcctcac tggcacagta cattgatgat ccagaagaag
    63481 tgtggtttgt gaaatcacca aaatcgttcc tcggtgccag cggcttaaaa ccgcagcagg
    63541 tagcgctgtt tgaggatctg gtctgcgcaa tgatgttgca cattcgccag caggcgcagg
    63601 cgcagctgcc agaagcgatt actcaggcgg tgattggtcg cccgatcaac ttccaggggc
    63661 tgggcggcga tgaagcaaac gcccaggcgc aagagattct ggaacgcgcg gcgaagcgtg
    63721 ccggattcag ggacgtggta ttccagtacg agccgatcgc ggctgggctg gattacgaag
    63781 ccaccttgca ggaagaaaaa cgggtgctgg tggtggatat cggcggtggt acgactgact
    63841 gttcattgct gctgatgggg ccgcagtggc gttcgcgtct cgatcgtgaa gccagtctgc
    63901 tgggtcacag tggttgtcgt attggcggta acgatctgga tatcgccctg gcgtttaaaa
    63961 acctgatgcc actgctgggt atgggtggcg aaaccgaaaa aggcatcgcc ctgccgatcc
    64021 tgccgtggtg gaatgcggtt gccatcaacg acgtacctgc gcagagtgat ttctacagta
    64081 gtgccaacgg tcgtctgctt aacgatctgg tacgcgatgc ccgcgaaccg gaaaaagtgg
    64141 ccctgttaca gaaagtctgg cgtcagcgtt taagctatcg cctggtgcgt agcgcagaag
    64201 agtgcaaaat tgctctttca agcgtagcgg aaacccgcgc ctcactgccg tttatcagca
    64261 atgaactggc tacgctgatt agccagcgag ggctggaaag cgccctcagc cagccactgg
    64321 cgcggattct ggaacaggtg caactggccc tggataacgc ccaggaaaaa ccggacgtta
    64381 tctacctgac cggcggtagc gcccgttctc cgcagattaa aaaagcgctg gcagaacagt
    64441 tgccgggcat tccgattgca ggcggcgatg actttggctc cgtcaccgcc gggctggcac
    64501 gctgggcgga agtggtgttt cgttaattga aaaatggctc agcagcggct taaatctgcc
    64561 tgcgcctgag ccagtttttc tgctgcctga cgtaacggtt ccatcaacgg catggtccgt
    64621 gctggagcat gaaggcggaa tcgctgcaac tctgtcgcgc cgactgtcaa cgccgattcc
    64681 agccgatgtc gtttggcggt cattttcgcc attaccgctt gtctgtctgc tggcgttatc
    64741 gcttcattcg gcctgaacac aaaacggcgt tcacagttcg ctttccagtc gataaccgcc
    64801 tgggtcagat ggtcgccaaa ccctttcact tgcttaacgc cacgacgggt aacatctgcg
    64861 gctgtttcaa taccaaaaga ccgtaacgcc gctttacgcg cagggccaac gccgggaata
    64921 gaggcaacat caataaaaaa tccctccaga aacttctgct tctgccgttc ccttgcagtg
    64981 tcatgaagtg ctgccagagc gcgtttctct tcttccggta acccgagaat ttcgtccttc
    65041 attttttcga gcatcgtccg tttggcgata aaaccttcca ggccgcctac ctgctggatc
    65101 tggcggacta aatggtcata gtccattttc gcacgattaa atgcctcacg gcgttgctga
    65161 acttctgctt tgtacgcttt gcttgtcaaa ctgccaataa tccagatagc cgccagcaca
    65221 ggaaccaaaa taatataacg cggttctgcc tgaaggccgc aaagcagcag cgataatgct
    65281 gacagtgcga tctcaagcag aatgatgtat tcgcgccgta acaggcctaa aggaagcggc
    65341 ctgccagtcg gttggaaatg atcgggtaat ggcagttgca atgctggcgg tgctactgac
    65401 gccatcacca tcgcccagac ttttgccagc acaaaatccc cgccggtggt aatgacctct
    65461 tcgccgagat caataaaata gataacacct tgattatcca gcgcacacca tgggcagtca
    65521 gccaaatgac cgggataaac atgcattgcc gaaacggtac atttctttaa ctgttggcgt
    65581 agagaatcca gtgctgatac ccacgcttta gccgtcggac gcccggttgc cacgccactt
    65641 tccgtaaacg cctgctgaaa catggcttca acatcactcg gtaacatcga tagcggaatc
    65701 gatcgtggcg gcggttttaa accacgtcgc tgattatctg acgcgtaggc ataacggaaa
    65761 tgggtaatat ccgtctccag cgcattaccc gcatcagaga taagcggcac accagaataa
    65821 ggatgccggc caccaaacaa gacgtgaaaa atcagcaacg caaggccaaa attatcgtga
    65881 ttttctgtgc gttcaaagcc gacaaatgac gacaaggttt gtagctctgg cggcgtaaaa
    65941 tgcgacacgc cgacttcgca taaatgcagt gtgccattgg cgttaatctg aaaggagtcg
    66001 ctatcaatca acaccacttt gctgtcgcga cctaccataa aactgttctg gtttacgtca
    66061 cccacgacgt gcccgtgctc gtgaaccgta gcaaaagatg aagcaatatt gcgcgcaaca
    66121 tagagtagaa aatcccacgc acaatgaggg taacgttgac gacgatgtgc cgggctatag
    66181 atcatatgaa tcggttcttt accagaaact tttggcatca taaaaccgat aacttttccg
    66241 cctcgtccac cgtgaagcgt tgcctgcggc caggcgacat aattcaacaa ttgcgcgtca
    66301 gctgtcgcag ccataaaggc aagtttgtcc tgttttaagg cgggtggcgg cgtgtgataa
    66361 atcttggcga cgctatcgac aaactcattg atatcataaa ccgcgccttc accgcctttg
    66421 cccagttcac ggcctggcgt aacgcactca ccagtagcag tatataaagt gggtttcatg
    66481 gtaaccacag cgccagtgcc aacgttttat catcatccgt acgctcgttt accgccgggc
    66541 tggacaaaaa ctgctttaac aattcaggta ataaatcgag ttgttcctgc gttgctgacg
    66601 ccagtccatt gaaaaacggg gtaaagaaag gcacatgagg agaattatcc agcatattta
    66661 acgccagccg ctgaatacca tccgtaaatg ctgcaacttt atgcgcacgc ccagtgctgg
    66721 tgaaagtttc cagtctggaa acggcatctt cgtcggtaat gaagtgcgtc atgttggcat
    66781 attcacccac catcggcgtg agaggcaatt gcagaccgtg acccaaatct accaccacgc
    66841 cgccatcgcc aatctgcata atcaaggtgc cgtcaggcga tgaaatcaat cccagaaacg
    66901 tgcaggcaaa atcgcgaact gctaatgctt tggcttctgc ctcggcaaaa agtcgttgac
    66961 gaatagtcag taccatattt gtggcgagga catcattaag ccccaattca ccgtcctgta
    67021 ctttttgcac cataaacgcc atcgcttcat tgacggcgag cgtcgcgcct tctcctccct
    67081 gcgaaacgct acctgcgcca tcagcaagga acatcaccag taatggttgc tggtcgttta
    67141 accagacaac ctgcatctgg caggcatcct gacaaggcaa atcagcacta atgtgcgatg
    67201 ttccaacagc ggatgcataa accagacgcc agctcacact gacgtccagc cttttggcgc
    67261 ttccagcaca acttccgttc ccggcgtgga gcgggaaacc gaacgcagcg aactggataa
    67321 ccagctaaac agttcacgga attgtaatcc ctgcagtggc aaaggctgac gaacgctgat
    67381 ttgtgccaga gtcttcatat ccgccccctg aacaccgatg gaaaagaagg caaacttctt
    67441 atcttcttcc ccctgaaaca ctttgttggc ggcagcctgc cactcatcgg ttggagcgcc
    67501 atcggtgatc aggaaaatcc acgggcgata atacgaaata ccatttgcgc ggtactcacg
    67561 tttcctctct tcaaccatat ccagggcttt ggtaatggcg gcccccatag gcgtatcccc
    67621 ctgggcaaac aggatgggcg ggaaaaagtt agcggcgctg gtaaagggtt gttccacatg
    67681 caccgggccg aacgtcacaa tgccgagttc cactcttttt aaagccagcg gatcagcaag
    67741 cagttcatca cgaaaggtaa ccaatccggc gttaagttca ttaataggtc tgccgctcat
    67801 cgagccagaa acatccagca gcaaaatgca ggggcaacgt ggttccgggt tactggcaaa
    67861 atcgctggtg gcaaacgtga tttgttcgct cataagaaat cctttcttag agaattatcg
    67921 aggtccggta tcataccgat tgcaataatg aaataagagc aaacgaagaa actttcagaa
    67981 ttaggcccgt cggcgcagaa aattttgcta ttcgcctcgc aagaacggag agccgtcgat
    68041 tgacactaag ggcggagtga cataatttca ggagtgaggg ttagggagag gtttccccct
    68101 ccccctggtg ttcttagtaa gcctggaagc taatcactaa gagtatcacc agtatgatga
    68161 cgtgcttcat cataaccctt tccttattaa aagccctctt ctccgggaga gggtttcccg
    68221 tttcagcgtc ccgctgaaat cgtcggctta cctcctttcg ccctgaatgc agtctatcgc
    68281 taacgcgtga acaaaatagt ttcatctgtg attattgcgt aacgactcgc aaagaatatt
    68341 tgctggcagg atcgcagact acaaagcctg cggattgaca atcttatcgt gaaggcataa
    68401 tttcaggagt gagggtagag cggggtttcc cccgccctgg tagtcttagt aagcggggaa
    68461 gcttatgact aagagcacca cgatgatgag tagcttcatc atgacccttt ccttattaag
    68521 gccccttcct cgggaggggc tttcccgttt cagcgtcccg ctgaaatcgt cggcttacct
    68581 cctttcgccc tgtatgcagt ctatcgctga cttgcggata aaatagtttc cagtgtgatt
    68641 attgcgtaac gtctcgcaaa gaatatttgc tggcaggatc gcagactgca aagcctgcag
    68701 attgacaatc ttatcgtgaa ggcatacttt caggagtgag ggtagagcgg ggtttccccc
    68761 gccctggtag ccttagtaag ctggccagct aatgactaag agcaccacga tgatgagtag
    68821 cttcatcatg accctttcct tatttatggc cccttcctcg ggaggggctt tcccgtttca
    68881 gcgtcccgct gaaatcggcg gcttacctcc tttcgccatg caagcagtct atcgctaacg
    68941 cgtagataaa atagtttcct gtgttattac tggatgcgtg ctcgcaaatg tgcccgtcat
    69001 tcagacgatt cccgacagtg tttcataatt cctccatttt tctcccttat tggctggcta
    69061 cactagtatc attccgcgaa acgtttcagg aagagaaact cttaacgatg aaaggcagtt
    69121 ataaatcccg ttgggtaatc gtggtggtta tcgccgccat cgccgcattc tggttctggc
    69181 aaggccgcaa tgactcccag agtgcagccc caggggcgac gaaacaagcg cagcaatcgc
    69241 cagcgggtgg tcgccgtggt atgcgttccg gcccattagc cccggttcag gcggcgaccg
    69301 ccgtagaaca ggcagttccg cgttacctca ccgggcttgg caccattatc gccgctaata
    69361 ctgttacggt gcgcagccgc gtggacggtc aactgatggc gttacatttc caggaaggcc
    69421 agcaggtcaa agcaggcgat ttactggcag aaattgaccc cagccagttc aaagttgcat
    69481 tagcacaaac ccagggccaa ctggcaaaag ataaagccac gcttgctaac gcccgccgtg
    69541 atctggcgcg ttatcagcaa ctggcaaaaa ccaatctcgt ctcccgtcag gagctggatg
    69601 cccaacaggc gctggtcagt gaaaccgaag gcaccattaa ggctgatgaa gcaagcgtcg
    69661 ccagcgcgca gttgcaactc gactggagcc gcatcaccgc accagtcgat ggtcgcgttg
    69721 gtctcaagca ggttgatgtt ggtaaccaaa tctccagtgg tgataccacc ggaattgtgg
    69781 tgatcaccca gacgcatcct atcgatttag tctttaccct gccggaaagc gatatcgcta
    69841 ctgtagtgca ggcgcaaaaa gccggaaaaa cgctggtggt agaagcctgg gatcgcacca
    69901 attcgaagaa attaagtgaa ggcacgctgt taagcctcga taaccaaatc gatgccacaa
    69961 ccggtacgat taaagtgaaa gcacgcttta ataatcagga tgatgcgctg tttcccaatc
    70021 agtttgttaa cgcgcgcatg ttagtcgaca ccgaacaaaa cgccgtagtg atcccaacag
    70081 ccgccctgca aatgggcaat gaaggccatt ttgtctgggt gctgaatagc gaaaacaagg
    70141 tcagcaaaca tctggtgacg ccgggcattc aggacagtca gaaagtggtg atccgtgcag
    70201 gtatttctgc gggcgatcgc gtggtgacag acggcattga tcgcctgacc gaaggggcga
    70261 aagtggaagt ggtggaagcc cagagcgcca ccactccgga agagaaagcc accagccgcg
    70321 aatacgcgaa aaaaggagca cgctcctgat gcaggtgtta cccccgagca gcacaggcgg
    70381 cccgtcgcgc ctgtttatta tgcgtcctgt cgccaccacg ctgctgatgg tggcgatctt
    70441 actcgccggg attatcggtt atcgcgccct gcccgtttcg gcgctgccgg aagtggacta
    70501 tccgaccatt caggtggtca cgctctaccc aggtgccagc ccggatgtca tgacctctgc
    70561 cgttaccgcg ccgctcgaac gccagttcgg gcagatgtct ggcctgaaac agatgtcgtc
    70621 gcaaagttcc ggcggtgcgt cagttatcac tttgcagttc cagctaacat taccgctcga
    70681 tgtcgccgag caggaagtgc aggccgcgat taacgctgcg accaacttgt tgccgagcga
    70741 tctgcctaac ccgccggttt acagcaaagt gaacccggca gatccgccga tcatgacgct
    70801 cgccgtcacc tcaaccgcca tgccgatgac gcaagtggaa gatatggtgg aaacccgcgt
    70861 cgcgcagaaa atctcgcaga tttccggcgt cggcctggtg acgctttccg gcggtcagcg
    70921 tccggctgtt cgcgtcaaac ttaacgctca ggcgattgcc gccctcggcc tgaccagcga
    70981 aaccgtgcgc accgccatta ccggcgctaa cgttaactcg gcaaaaggta gcctcgacgg
    71041 cccttcccgt gcggtcacgc tttccgcgaa cgaccagatg caatccgccg aagagtatcg
    71101 ccagctaatc atcgcctacc agaacggcgc gccaattcgt ctgggcgatg tcgcaactgt
    71161 agagcaaggt gcagaaaaca gctggctcgg cgcgtgggcg aacaaagaac aggccattgt
    71221 gatgaatgtt cagcgccagc ccggtgctaa cattatctcc accgccgaca gcattcggca
    71281 gatgctgcca cagctcactg agagtctgcc gaaatcggtg aaggtgacag tgctttccga
    71341 tcgcaccacc aatatccgcg catccgtcga tgatactcag tttgaattga tgatggctat
    71401 cgcgctggta gtcatgatta tctacctgtt tttgcgcaat attccggcga ccatcattcc
    71461 cggtgttgct gtaccgctgt cgttaatcgg cactttcgcg gttatggtgt ttctcgattt
    71521 ttcaatcaat aacctgacac tgatggcgtt aactatcgcc accggattcg tggtcgatga
    71581 cgccatcgtg gtgatcgaaa acatttcccg ctatatcgaa aaaggcgaaa aaccgttggc
    71641 ggcggcgctc aagggcgcag gtgaaatcgg ctttaccatt atctcgctga ccttctcact
    71701 gattgcggtg ttgatcccac tgctgtttat gggcgatatc gtcgggcgac tgttccgcga
    71761 atttgctatt accctggcgg tagcgatttt gatctcagcg gtggtgtcgc tgaccctgac
    71821 accgatgatg tgcgcgcgga tgctcagcca ggagtcgttg cgtaaacaga accgcttctc
    71881 ccgtgcctcg gaaaaaatgt tcgacaggat aatcgccgcc tatggtcgtg gactggcgaa
    71941 agtgctgaat catccgtggc tgaccttaag cgtggcactc agcacgctgc tgcttagcgt
    72001 gctgctgtgg gtgttcattc cgaaaggttt cttcccggta caggacaatg gcattattca
    72061 gggcactttg caggcaccgc aatccagctc ctttaccaat atggcccagc gacaacgcca
    72121 ggtcgcggac gtgattttgc aggatccggc agtgcaaagc ctgacctcat ttgttggcgt
    72181 tgatggcact aacccgtcgc tgaatagtgc acgtttacaa atcaacctca aaccgttgga
    72241 tgaacgtgat gatcgggtgc aaaaagtcat cgcccgtctg caaacggcgg tagataaagt
    72301 gccgggcgtc gatctcttcc tgcaaccaac gcaggatctg actattgata ctcaggtcag
    72361 ccgcacccag taccagttta ccttgcaggc cacctcactg gatgcgctca gtacctgggt
    72421 gccacagttg atggaaaaac tccagcaact gccacagctt tctgatgtct ccagcgactg
    72481 gcaggaccaa gggctggtgg cgtatgtcaa tgttgatcgc gacagcgcca gccgtctggg
    72541 gatcagcatg gcggatgtcg ataacgccct gtacaacgcg tttggtcagc gactgatttc
    72601 cactatttat actcaggcta accagtatcg cgtagtgctg gagcacaaca ccgaaattac
    72661 cccgggcctc gcggcgctgg ataccattcg cctgaccagt agcgacggcg gcgtggtgcc
    72721 gttaagctca attgccaaag ttgaacagcg ttttgcgccg ctttccatca accatctgga
    72781 tcagttcccg gtaacgacca tctcctttaa cgtgccggat aactattcgc tgggcgatgc
    72841 ggtgcaggag attatggaca ccgaaaagac gctgaatctg ccggtggata tcaccacgca
    72901 attccagggc agcaccctcg ccttccagtc ggcgctgggc agcactgtct ggctgattgt
    72961 cgcggcggtg gtggcgatgt atatcgtgct cggcattctg tacgagagct ttattcaccc
    73021 gatcaccatt ctctcgacgc tacccaccgc aggggtcggc gcgctgctgg cattaatgat
    73081 tgccggtagc gaactggatg tgattgcgat tatcggcatt attttgctga tcggtattgt
    73141 gaagaagaac gccatcatga tgatcgactt cgcgctggct gctgagcgcg agcaaggcat
    73201 gtcgccgcgc gatgcaatct accaggcttg tctgttgcgt tttcgtccga tcctgatgac
    73261 caccctggcg gctctgcttg gcgcgctgcc gctgatgttg agtaccgggg tcggcgcgga
    73321 actgcgtcgt ccgttaggta tcggcatggt cggcggtctg attgtcagcc aggtgctgac
    73381 gctgttcacc acgccggtga tttatctgct gttcgaccgc ctggcattgt ggaccaaaag
    73441 tcgctttgct cgccatgaag aggaggcgta agtgaagttt tttgccctct tcatttaccg
    73501 cccggtggcg acgattttac tgtcggtcgc cattaccctg tgcggcatac tgggcttccg
    73561 tatgctgccg gtcgccccgc tgccgcaggt cgattttccg gtgattatgg tcagcgcctc
    73621 gctgcccggt gcgtcaccag aaacaatggc gtcttccgtt gccacgccgc tggagcgctc
    73681 gcttgggcgc attgccgggg tcagcgaaat gacctccagc agttcgctcg gcagcacacg
    73741 tattatttta cagtttgatt ttgaccggga catcaacggc gcagcgcgtg atgtgcaggc
    73801 ggcgatcaac gctgcacaaa gtttgctgcc cagtgggatg cccagccgtc cgacctatcg
    73861 caaagcgaac ccgtcggatg cgccaattat gatcctcacg ctgacatccg atacttattc
    73921 gcagggtgaa ctgtacgatt tcgcctcgac gcagctggct ccgacgattt cgcaaatcga
    73981 cggtgttggt gatgtcgatg tcggaggcag ctcactgccc gccgtacgcg tcgggctgaa
    74041 tccgcaggcg ctgtttaatc agggcgtgtc gctggacgac gtacgcaccg ccatcagcaa
    74101 tgccaacgtg cgtaaaccgc agggcgcgct ggaagatggc actcaccgct ggcagatcca
    74161 gaccaatgat gagctaaaaa ccgccgctga atatcagccg ttgattattc actacaacaa
    74221 cggcggcgcg gttcgtctgg gcgatgtggc gacggtgacc gactcagtgc aggatgtgcg
    74281 caacgccggg atgaccaacg ccaaaccggc tattttactg atgatccgca aactgccgga
    74341 agccaatatt atccagacgg ttgacagcat ccgggcaaaa ttaccggagt tgcaggaaac
    74401 cattccggcg gcgattgatc tgcaaattgc ccaggatcgc tcccccacca ttcgcgcctc
    74461 gctggaagaa gtcgagcaaa cgctgattat ctcggtggcg ctggtgattc tggtggtgtt
    74521 tttattcctg cgctcgggtc gcgccactat tattcccgcc gtttcggtgc cggtttcgct
    74581 gattggtacg tttgcggcga tgtacctgtg cggattcagt ctcaataacc tttcgttaat
    74641 ggcgctcacc atcgctactg gtttcgtggt ggatgacgcc atcgtggtgc tggaaaacat
    74701 tgcacgtcat ctggaagcgg gaatgaaacc gttgcaagcc gcactgcaag gtactcgcga
    74761 agtcggtttt acggtgctgt cgatgagtct gtcactggtg gcggtgttcc tgccgctgct
    74821 gttgatgggc ggattgccgg gccgactgtt acgcgagttt gccgtgacgc tttctgtcgc
    74881 cattggtatt tcgttgctgg tttctctgac attaacgcca atgatgtgtg gctggatgct
    74941 gaaagccagc aagccgcgcg agcaaaagcg actgcgtggt tttggtcgca tgttggtagc
    75001 cctgcaacaa ggctacggca agtcactgaa atgggtgctc aatcataccc gtctggtggg
    75061 cgtggtgctg cttggcacca ttgcgctgaa tatctggctg tatatctcga tcccgaaaac
    75121 cttcttcccg gagcaggaca ctggcgtgtt gatgggcggg attcaggcgg atcagagtat
    75181 ttcgtttcag gcgatgcgcg gtaagttgca ggatttcatg aaaattatcc gtgacgatcc
    75241 ggcagtggat aatgtcaccg gctttacagg cggttcgcga gtgaacagcg ggatgatgtt
    75301 tatcaccctc aagccacgcg acgaacgcag cgaaacggcg cagcaaatta tcgaccgtct
    75361 gcgcgtaaaa ctggcgaaag aaccgggggc gaatctgttc ctgatggcgg tacaggatat
    75421 tcgcgttggt gggcgtcagt cgaacgccag ctaccagtac acgttgttat ccgacgacct
    75481 ggcggcactg cgcgaatggg agccgaaaat ccgcaaaaaa ctggcgacgt tgccggaact
    75541 ggcggacgtg aactccgatc agcaggataa cggcgcggag atgaatctgg tttacgaccg
    75601 cgacaccatg gcacggctgg gaatcgacgt gcaggccgcc aacagcctgt taaataacgc
    75661 cttcggtcag cggcaaatct cgaccattta ccagccgatg aaccagtata aagtggtgat
    75721 ggaagtggat ccgcgctata cccaggacat cagtgcgctg gaaaaaatgt tcgttatcaa
    75781 taacgaaggc aaagcgatcc cgctgtcgta tttcgctaaa tggcaaccgg cgaacgctcc
    75841 gctgtcggtg aatcatcagg gattatcggc ggcctcgacc atctcgttca acctgccgac
    75901 cggaaaatcg ctctcggacg ccagtgcggc gatcgatcgc gcaatgaccc agcttggtgt
    75961 gccttcgacg gtgcgcggca gttttgccgg aacagcgcag gtgttccagg agacgatgaa
    76021 ctcacaggtg atcctgatta tcgccgccat cgccacggtg tatatcgtgc tgggtatcct
    76081 ttacgagagt tacgtacacc cactgacgat tctctccacc ctgccctcgg cgggcgttgg
    76141 tgcgctgctg gcgctggagc tgttcaatgc cccgttcagc ctaatagccc tgatagggat
    76201 catgctatta atcggcatcg taaagaaaaa cgccattatg atggtcgatt ttgcgctcga
    76261 agcccaacgg cacggtaacc tgacgccgca ggaagctatt ttccaggcct gtctgctgcg
    76321 ttttcgcccg attatgatga ctaccctggc ggcgctgttt ggcgcgctgc cgctggtgtt
    76381 gtcgggcggt gacggctcgg agctgcggca cctcctgggg atcaccattg tcggtggact
    76441 ggtaatgagc cagcttctga cgctgtatac cacgccggtg gtgtatctct ttttcgaccg
    76501 tctgcggctg cgtttttcgc gtaaacctaa acaaacggta accgagtaaa tgacagaact
    76561 tcccgacagc acccgctggc aattgtggat tgtggctttc ggcttcttta tgcagtcgct
    76621 ggacaccacc atcgttaaca ccgcccttcc ctcaatggcg caaagcctcg gggaaagtcc
    76681 gttgcatatg cacatggtca ttgtctctta tgtgctgacc gtggcggtga tgctgcccgc
    76741 cagcggctgg ctggcggaca aagtcggcgt gcgcaatatt ttctttaccg ccatcgtgct
    76801 gtttactctc ggttcactgt tttgcgcgct ttccggcacg ctgaacgaac tgttgctggc
    76861 acgcgcgtta cagggcgttg gcggtgcgat gatggtgccg gtcggcaggt tgacggtgat
    76921 gaaaatcgta ccgcgcgagc aatatatggc ggcgatgacc tttgtcacgt tacccggtca
    76981 ggtcggtccg ctgctcggtc cggcgctcgg cggtctgctg gtggagtacg cgtcgtggca
    77041 ctggatcttt ttgatcaaca ttccggtggg gattatcggt gcgatcgcca cattgctgtt
    77101 aatgccgaac tacaccatgc agacgcggcg ctttgatctt tccggctttt tattgctggc
    77161 ggttggcatg gctgtattaa ccctggcgct ggacggcagt aaaggtacag gtttatcgcc
    77221 gctggcgatt gctggcctgg tcgcagttgg cgtggtggca ctggtgcttt atctgctgca
    77281 cgccagaaat aacaaccgcg ccctgttcag tctgaaactg ttccgcactc gtaccttttc
    77341 gctgggcctg gcggggagct ttgccggacg tattggcagt ggcatgttgc cctttatgac
    77401 accggttttc ctgcaaattg gcctcggttt ctcgccgttt catgccggac tgatgatgat
    77461 cccgatggta cttggcagca tgggaatgaa gcgaattgtg gttcaggtgg tgaatcgctt
    77521 tggttatcgt cgggtactgg tagcgaccac gctgggtctg tcgctgatca ccctgttgtt
    77581 tatgactacc gctctgttgg gctggtacta cgttttgccg ttcgtcctgt ttttacaagg
    77641 gatggtcaac tcgacccgtt tctcctccat gaacaccctg acgctgaaag atctcccgga
    77701 caatctggcg agcagcggca acagtctgct gtcgatgatt atgcaattgt cgatgagtat
    77761 cggcgtcacc atcgccgggc tgttgctggg actttttggt tcgcagcatg tcagcgtcga
    77821 cagcggcacc acacaaaccg tctttatgta cacctggctt agcatggcgt ttatcatcgc
    77881 ccttccggcg ttcatctttg ccagagtgcc gaacgatacg catcaaaatg tagctatttc
    77941 gcggcgaaaa aggagcgcgc aatgaagttc tggcgacctg gtattaccgg caaactgttt
    78001 ctggcgattt tcgccacctg cattgttttg ctgatcagta tgcactgggc ggtgcgtatc
    78061 agttttgagc gtggctttat tgattacatc aagcatggta atgaacagcg attacaactg
    78121 ttaagtgatg cgcttggcga gcagtatgcg cagcatggca actggcgctt cctgcgcaac
    78181 aatgatcgct ttgtctttca gatcctgcgt tcatttgaac acgataattc ggaagataaa
    78241 cccggcccgg gtatgccacc gcacggctgg cgtacccagt tctgggtggt tgatcaaaac
    78301 aacaaagtgc tggttggtcc gcgagcgccg attccacctg acggtacacg gcgacccatt
    78361 ctggtcaacg gtgcggaagt tggcgcggtg atcgcctccc ccgttgagcg gttaacgcgc
    78421 aatactgata tcaatttcga taaacaacag cggcaaacca gctggttgat tgtcgccctg
    78481 gcaacgttac tcgcggcact tgccactttt ctgctggcgc gcggtttact ggcaccggta
    78541 aaacgacttg tcgatggcac gcacaaactg gcggcgggcg atttcactac ccgcgtaacg
    78601 cccaccagtg aagatgaact gggcaaactg gcgcaagact tcaaccagct tgccagcaca
    78661 ctggagaaaa accagcaaat gcggcgcgat tttatggccg atatttctca cgaactgcgt
    78721 acgccattag cggtgctgcg cggtgaactg gaagctattc aggatggcgt gcgcaaattc
    78781 acgccggaga cggtggcgtc tttacaggcg gaggtcggta cactgaccaa actggttgac
    78841 gatctccatc agttgtcgat gtctgatgaa ggcgccctcg cctatcaaaa agcaccggta
    78901 gatttgatcc cactgctgga agtggcgggc ggcgcatttc gcgaacgatt cgccagccgt
    78961 ggcctgaaac tgcaattttc cctgccagac agtattaccg tatttggcga tcgcgaccgt
    79021 ttaatgcagt tattcaataa tttacttgaa aacagcctgc gctacactga cagcggcggc
    79081 agcctgaaaa tctctgccga gcagcacgac aaaacggtgc gcctgacctt tgccgacagt
    79141 gcgccaggtg tcagtgacga gcagctacaa aaattatttg aacgttttta tcgcaccgaa
    79201 ggttcccgca accgcgccag cggtggttcc gggctggggc tggcgatttg cctgaacatt
    79261 gttgaagcac ataatggccg cattattgct gcccattcgc cttttggcgg ggtaagcatt
    79321 acagtagagt taccgctgga acgggattta cagagagaag tatgaccgag ttaccaatcg
    79381 acgaaaacac accgcgtatt ttgatcgtgg aagatgaacc gaagctgggg cagttgctca
    79441 ttgattatct gcgtgctgcg agctatgcgc cgacgcttat cagccacggc gatcaggtac
    79501 tgccgtatgt gcgccagaca ccaccggatc tgatcctgtt ggatctgatg ctccctggca
    79561 ccgatggctt gacgctgtgc cgggaaattc gtcgtttttc tgacattcct atcgtgatgg
    79621 tgacggcaaa aatcgaagag atcgaccgcc tgctcggact ggagattggc gcagatgact
    79681 acatctgcaa gccgtacagc ccacgggaag tggtagcgcg cgtcaaaacc attttgcgcc
    79741 gttgcaaacc gcagcgcgaa ttgcagcaac aggatgccga aagcccgttg attatcgacg
    79801 aaggtcgttt tcaggcttca tggcgcggta aaatgcttga cctgacgcct gcggaatttc
    79861 gtctgttgaa aacgctctct catgaaccag gaaaagtgtt ctcccgcgag caattgctca
    79921 atcatcttta tgacgactac cgcgtagtaa ccgaccgcac cattgacagc cacattaaaa
    79981 acctgcgccg caagctggaa tctctcgacg ccgaacagtc atttatccgc gccgtttacg
    80041 gcgttggtta ccgctgggaa gccgacgcct gccgcatcgt ttagttttag cgacatgact
    80101 ttgttagccg gagatggcgt tcagaccaga tccggcaaca ttatcccacg catggtcagc
    80161 aaactga
//
LOCUS       DAEEKQ010000003        75780 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.003, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000003 DAEEKQ010000000
VERSION     DAEEKQ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.003"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(81..1331)
                     /gene="icd"
                     /locus_tag="KLK57_000171"
     CDS             complement(81..1331)
                     /gene="icd"
                     /locus_tag="KLK57_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HBE0890662.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGEAIIENM"
     gene            1503..2156
                     /gene="rluE"
                     /locus_tag="KLK57_000172"
     CDS             1503..2156
                     /gene="rluE"
                     /locus_tag="KLK57_000172"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309634.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HBE0890663.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDTEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYTLDNLANGEWREATE"
     gene            2166..2627
                     /gene="nudJ"
                     /locus_tag="KLK57_000173"
     CDS             2166..2627
                     /gene="nudJ"
                     /locus_tag="KLK57_000173"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HBE0890664.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2681..3787
                     /gene="mnmA"
                     /locus_tag="KLK57_000174"
     CDS             2681..3787
                     /gene="mnmA"
                     /locus_tag="KLK57_000174"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HBE0890665.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPGQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3823..4464
                     /gene="hflD"
                     /locus_tag="KLK57_000175"
     CDS             3823..4464
                     /gene="hflD"
                     /locus_tag="KLK57_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HBE0890666.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4468..5838
                     /gene="purB"
                     /locus_tag="KLK57_000176"
     CDS             4468..5838
                     /gene="purB"
                     /locus_tag="KLK57_000176"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HBE0890667.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6006..6677
                     /gene="phoP"
                     /locus_tag="KLK57_000177"
     CDS             6006..6677
                     /gene="phoP"
                     /locus_tag="KLK57_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HBE0890668.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6677..8137
                     /gene="phoQ"
                     /locus_tag="KLK57_000178"
     CDS             6677..8137
                     /gene="phoQ"
                     /locus_tag="KLK57_000178"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HBE0890669.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8213..9334
                     /gene="roxA"
                     /locus_tag="KLK57_000179"
                     /gene_synonym="ycfD"
     CDS             8213..9334
                     /gene="roxA"
                     /locus_tag="KLK57_000179"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HBE0890670.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9383..10609)
                     /gene="pepT"
                     /locus_tag="KLK57_000180"
     CDS             complement(9383..10609)
                     /gene="pepT"
                     /locus_tag="KLK57_000180"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HBE0890671.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10859..11995
                     /gene="potA"
                     /locus_tag="KLK57_000181"
     CDS             10859..11995
                     /gene="potA"
                     /locus_tag="KLK57_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HBE0890672.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11979..12836
                     /gene="potB"
                     /locus_tag="KLK57_000182"
     CDS             11979..12836
                     /gene="potB"
                     /locus_tag="KLK57_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HBE0890673.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12833..13627
                     /gene="potC"
                     /locus_tag="KLK57_000183"
     CDS             12833..13627
                     /gene="potC"
                     /locus_tag="KLK57_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HBE0890674.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13624..14670
                     /gene="potD"
                     /locus_tag="KLK57_000184"
     CDS             13624..14670
                     /gene="potD"
                     /locus_tag="KLK57_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HBE0890675.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKAIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(14826..15647)
                     /gene="cobB"
                     /locus_tag="KLK57_000185"
     CDS             complement(14826..15647)
                     /gene="cobB"
                     /locus_tag="KLK57_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HBE0890676.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(15663..16574)
                     /gene="nagK"
                     /locus_tag="KLK57_000186"
     CDS             complement(15663..16574)
                     /gene="nagK"
                     /locus_tag="KLK57_000186"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HBE0890677.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQIGCIENYLSGRGFAWLWQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDSDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(16603..17847)
                     /gene="lolE"
                     /locus_tag="KLK57_000187"
     CDS             complement(16603..17847)
                     /gene="lolE"
                     /locus_tag="KLK57_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HBE0890678.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGTNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIERIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(17847..18548)
                     /gene="lolD"
                     /locus_tag="KLK57_000188"
     CDS             complement(17847..18548)
                     /gene="lolD"
                     /locus_tag="KLK57_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HBE0890679.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(18541..19740)
                     /gene="lolC"
                     /locus_tag="KLK57_000189"
     CDS             complement(18541..19740)
                     /gene="lolC"
                     /locus_tag="KLK57_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HBE0890680.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGNFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            20002..21075
                     /locus_tag="KLK57_000190"
     CDS             20002..21075
                     /locus_tag="KLK57_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HBE0890681.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            21203..24649
                     /gene="mfd"
                     /locus_tag="KLK57_000191"
     CDS             21203..24649
                     /gene="mfd"
                     /locus_tag="KLK57_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HBE0890682.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            24793..25749
                     /gene="ldtC"
                     /locus_tag="KLK57_000192"
                     /pseudo
     CDS             24793..25749
                     /gene="ldtC"
                     /locus_tag="KLK57_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
     gene            complement(25832..26089)
                     /gene="bhsA"
                     /locus_tag="KLK57_000193"
     CDS             complement(25832..26089)
                     /gene="bhsA"
                     /locus_tag="KLK57_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HBE0890683.1"
                     /translation="MKNVKTLIAAAILSSMSFANFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            26330..26962
                     /gene="comR"
                     /locus_tag="KLK57_000194"
     CDS             26330..26962
                     /gene="comR"
                     /locus_tag="KLK57_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HBE0890684.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(27024..27563)
                     /locus_tag="KLK57_000195"
     CDS             complement(27024..27563)
                     /locus_tag="KLK57_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBE0890685.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(27790..29094)
                     /gene="ndh"
                     /locus_tag="KLK57_000196"
     CDS             complement(27790..29094)
                     /gene="ndh"
                     /locus_tag="KLK57_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HBE0890686.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELYNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMKNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(29502..30044)
                     /locus_tag="KLK57_000197"
     CDS             complement(29502..30044)
                     /locus_tag="KLK57_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0890687.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(30067..31092)
                     /gene="nagZ"
                     /locus_tag="KLK57_000198"
     CDS             complement(30067..31092)
                     /gene="nagZ"
                     /locus_tag="KLK57_000198"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HBE0890688.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSSYWLKTVLRQELCFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(31103..31927)
                     /gene="thiK"
                     /locus_tag="KLK57_000199"
     CDS             complement(31103..31927)
                     /gene="thiK"
                     /locus_tag="KLK57_000199"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HBE0890689.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQIGDQQFIRLADDTWRQLLIKQ"
     gene            complement(31908..32549)
                     /gene="lpoB"
                     /locus_tag="KLK57_000200"
     CDS             complement(31908..32549)
                     /gene="lpoB"
                     /locus_tag="KLK57_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HBE0890690.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(32563..32940)
                     /locus_tag="KLK57_000201"
     CDS             complement(32563..32940)
                     /locus_tag="KLK57_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HBE0890691.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVSIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(32943..33302)
                     /gene="hinT"
                     /locus_tag="KLK57_000202"
     CDS             complement(32943..33302)
                     /gene="hinT"
                     /locus_tag="KLK57_000202"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HBE0890692.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            33636..35825
                     /gene="fhuE"
                     /locus_tag="KLK57_000203"
     CDS             33636..35825
                     /gene="fhuE"
                     /locus_tag="KLK57_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HBE0890693.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQLDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTLGISKSQADSDRALYYSRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWANIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNMEGSIVYGAPRNFSITGTYQF"
     gene            complement(35885..37318)
                     /gene="ptsG"
                     /locus_tag="KLK57_000204"
     CDS             complement(35885..37318)
                     /gene="ptsG"
                     /locus_tag="KLK57_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HBE0890694.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(37613..38410)
                     /locus_tag="KLK57_000205"
     CDS             complement(37613..38410)
                     /locus_tag="KLK57_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE0890695.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(38421..39425)
                     /gene="holB"
                     /locus_tag="KLK57_000206"
     CDS             complement(38421..39425)
                     /gene="holB"
                     /locus_tag="KLK57_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HBE0890696.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVVE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(39422..40063)
                     /locus_tag="KLK57_000207"
     CDS             complement(39422..40063)
                     /locus_tag="KLK57_000207"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HBE0890697.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(40053..41075)
                     /gene="yceG"
                     /locus_tag="KLK57_000208"
     CDS             complement(40053..41075)
                     /gene="yceG"
                     /locus_tag="KLK57_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HBE0890698.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(41078..41887)
                     /gene="pabC"
                     /locus_tag="KLK57_000209"
     CDS             complement(41078..41887)
                     /gene="pabC"
                     /locus_tag="KLK57_000209"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HBE0890699.1"
                     /translation="MFLINGHKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(42007..43248)
                     /gene="fabF"
                     /locus_tag="KLK57_000210"
     CDS             complement(42007..43248)
                     /gene="fabF"
                     /locus_tag="KLK57_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HBE0890700.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLDITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(43336..43572)
                     /gene="acpP"
                     /locus_tag="KLK57_000211"
     CDS             complement(43336..43572)
                     /gene="acpP"
                     /locus_tag="KLK57_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HBE0890701.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(43783..44517)
                     /gene="fabG"
                     /locus_tag="KLK57_000212"
     CDS             complement(43783..44517)
                     /gene="fabG"
                     /locus_tag="KLK57_000212"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HBE0890702.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(44530..45459)
                     /gene="fabD"
                     /locus_tag="KLK57_000213"
     CDS             complement(44530..45459)
                     /gene="fabD"
                     /locus_tag="KLK57_000213"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HBE0890703.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKLMQEAVPEGTGAMAAIIGLDDASIAKACEEAAAGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(45475..46428)
                     /gene="fabH"
                     /locus_tag="KLK57_000214"
     CDS             complement(45475..46428)
                     /gene="fabH"
                     /locus_tag="KLK57_000214"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HBE0890704.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(46496..47566)
                     /gene="plsX"
                     /locus_tag="KLK57_000215"
     CDS             complement(46496..47566)
                     /gene="plsX"
                     /locus_tag="KLK57_000215"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HBE0890705.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRVALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(47647..47820)
                     /gene="rpmF"
                     /locus_tag="KLK57_000216"
     CDS             complement(47647..47820)
                     /gene="rpmF"
                     /locus_tag="KLK57_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HBE0890706.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(47872..48393)
                     /gene="yceD"
                     /locus_tag="KLK57_000217"
     CDS             complement(47872..48393)
                     /gene="yceD"
                     /locus_tag="KLK57_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HBE0890707.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            48592..49176
                     /gene="yceF"
                     /locus_tag="KLK57_000218"
     CDS             48592..49176
                     /gene="yceF"
                     /locus_tag="KLK57_000218"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HBE0890708.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(49288..50247)
                     /gene="rluC"
                     /locus_tag="KLK57_000219"
     CDS             complement(49288..50247)
                     /gene="rluC"
                     /locus_tag="KLK57_000219"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HBE0890709.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLIAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            50820..54005
                     /gene="rne"
                     /locus_tag="KLK57_000220"
     CDS             50820..54005
                     /gene="rne"
                     /locus_tag="KLK57_000220"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HBE0890710.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTGVPVAAAVEPVVSAPVVEEVAEVVEAPVQIAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEVTVEHNHATVPMTRAPAPEYVPDAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(54200..55153)
                     /gene="flgL"
                     /locus_tag="KLK57_000221"
     CDS             complement(54200..55153)
                     /gene="flgL"
                     /locus_tag="KLK57_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HBE0890711.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEANGDYVGGTESI
                     KQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKETAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(55165..56808)
                     /gene="flgK"
                     /locus_tag="KLK57_000222"
     CDS             complement(55165..56808)
                     /gene="flgK"
                     /locus_tag="KLK57_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HBE0890712.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVYVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITVANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQELDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFTIGKPA
                     VLQNTKNKGDVAIGATVTDASVVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEKDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(56874..57815)
                     /gene="flgJ"
                     /locus_tag="KLK57_000223"
     CDS             complement(56874..57815)
                     /gene="flgJ"
                     /locus_tag="KLK57_000223"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HBE0890713.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(57815..58912)
                     /gene="flgI"
                     /locus_tag="KLK57_000224"
     CDS             complement(57815..58912)
                     /gene="flgI"
                     /locus_tag="KLK57_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HBE0890714.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTESLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(58924..59622)
                     /gene="flgH"
                     /locus_tag="KLK57_000225"
     CDS             complement(58924..59622)
                     /gene="flgH"
                     /locus_tag="KLK57_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HBE0890715.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(59675..60447)
                     /gene="flgG"
                     /locus_tag="KLK57_000226"
                     /pseudo
     CDS             complement(59675..60447)
                     /gene="flgG"
                     /locus_tag="KLK57_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
     gene            complement(60619..61374)
                     /gene="flgF"
                     /locus_tag="KLK57_000227"
     CDS             complement(60619..61374)
                     /gene="flgF"
                     /locus_tag="KLK57_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HBE0890716.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQTADGSEGYTRN
                     GSIQVDPTGQLTIQGHQVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAETQATRGPVLQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(61394..62599)
                     /gene="flgE"
                     /locus_tag="KLK57_000228"
     CDS             complement(61394..62599)
                     /gene="flgE"
                     /locus_tag="KLK57_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HBE0890717.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(62624..63319)
                     /gene="flgD"
                     /locus_tag="KLK57_000229"
     CDS             complement(62624..63319)
                     /gene="flgD"
                     /locus_tag="KLK57_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HBE0890718.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVLRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(63331..63735)
                     /gene="flgC"
                     /locus_tag="KLK57_000230"
     CDS             complement(63331..63735)
                     /gene="flgC"
                     /locus_tag="KLK57_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HBE0890719.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(63739..64155)
                     /gene="flgB"
                     /locus_tag="KLK57_000231"
     CDS             complement(63739..64155)
                     /gene="flgB"
                     /locus_tag="KLK57_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HBE0890720.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            64310..64969
                     /gene="flgA"
                     /locus_tag="KLK57_000232"
     CDS             64310..64969
                     /gene="flgA"
                     /locus_tag="KLK57_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HBE0890721.1"
                     /translation="MLAIKRSVAIISILFTPLSAASNLTSQLQTFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRQDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVTQNARVRMVSGLVVSGVVDADGNILIN
                     Q"
     gene            65045..65338
                     /gene="flgM"
                     /locus_tag="KLK57_000233"
     CDS             65045..65338
                     /gene="flgM"
                     /locus_tag="KLK57_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HBE0890722.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDASVTNTRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALLNEAQQDLQSN"
     gene            65343..65759
                     /gene="flgN"
                     /locus_tag="KLK57_000234"
     CDS             65343..65759
                     /gene="flgN"
                     /locus_tag="KLK57_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HBE0890723.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRRKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(65799..67334)
                     /gene="murJ"
                     /locus_tag="KLK57_000235"
     CDS             complement(65799..67334)
                     /gene="murJ"
                     /locus_tag="KLK57_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HBE0890724.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(67444..68367)
                     /locus_tag="KLK57_000236"
     CDS             complement(67444..68367)
                     /locus_tag="KLK57_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE0890725.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSADPLQITTCMHRRAG
                     SQRETVQAVTDDALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVLQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(68369..69016)
                     /locus_tag="KLK57_000237"
     CDS             complement(68369..69016)
                     /locus_tag="KLK57_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HBE0890726.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(69027..69611)
                     /gene="rimJ"
                     /locus_tag="KLK57_000238"
     CDS             complement(69027..69611)
                     /gene="rimJ"
                     /locus_tag="KLK57_000238"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HBE0890727.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            69847..71055
                     /gene="mdtH"
                     /locus_tag="KLK57_000239"
     CDS             69847..71055
                     /gene="mdtH"
                     /locus_tag="KLK57_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HBE0890728.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRCRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSALLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     ATRRLLERDA"
     gene            71119..71766
                     /gene="grxB"
                     /locus_tag="KLK57_000240"
     CDS             71119..71766
                     /gene="grxB"
                     /locus_tag="KLK57_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HBE0890729.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            71900..72460
                     /locus_tag="KLK57_000241"
     CDS             71900..72460
                     /locus_tag="KLK57_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0890730.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLISQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            72566..73612
                     /gene="pyrC"
                     /locus_tag="KLK57_000242"
     CDS             72566..73612
                     /gene="pyrC"
                     /locus_tag="KLK57_000242"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HBE0890731.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            73686..73931
                     /gene="dinI"
                     /locus_tag="KLK57_000243"
     CDS             73686..73931
                     /gene="dinI"
                     /locus_tag="KLK57_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HBE0890732.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            74221..74475
                     /gene="bssS"
                     /locus_tag="KLK57_000244"
     CDS             74221..74475
                     /gene="bssS"
                     /locus_tag="KLK57_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415578.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HBE0890733.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFQVNEYRRH"
     gene            74590..75708
                     /gene="solA"
                     /locus_tag="KLK57_000245"
     CDS             74590..75708
                     /gene="solA"
                     /locus_tag="KLK57_000245"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HBE0890734.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
BASE COUNT        18416 a        20165 c        18954 g        18245 t
ORIGIN      
        1 gatatgctat taataatatc cacataaata aaacaacggg cgtgttatac gcccgtttca
       61 atatttaact catggagaga ttacatgttt tcgatgatcg cttcaccaaa ctctgaacat
      121 ttcagcagct tagcgccttc catcagacgt tcaaagtcat aagttacggt tttcgcgttg
      181 atagcacctt ccataccttt aacaattaaa tcagccgctt cagtccaccc catatggcgc
      241 agcatcatct cagcggagag gatgatagag cctggattta ctttgtcctg accggcatat
      301 ttcggcgcag taccgtgggt ggcttcaaac agggcgcatt cgtcaccgat gtttgcgcca
      361 ggggcgatac cgataccgcc aacctgcgct gccagggcgt cagaaatgta gtcaccgttc
      421 aggttcatac aggcgataac atcatattca gccggacgca gcaggatctg ttgcaggaat
      481 gcatcagcaa tcacgtcttt aatgacgatc tctttgccgg tgttcgggtt tttaactttc
      541 agccacgggc cgccgtcgat cagttcaccg ccaaactctt cacgcgccag ctggtagccc
      601 cagtctttaa acgcgccttc ggtgaacttc atgatgttgc ctttgtgcac cagagtcaca
      661 gagtcacgat cgttagcaat tgcgtattcg atcgctgcac gaaccagacg tttggtgcct
      721 tcttccgaac acggcttaat accgatacca caatgttccg ggaagcgaat tttcttcacc
      781 cccatctctt cacgcaggaa tttaatcact ttctcggcgt cggcagagtc tgctttccat
      841 tcgatacccg cataaatgtc ttccgagttt tcacggaaga taaccatatc ggtcagttca
      901 gggtgtttaa ccgggcttgg agtgccctga tagtaacgta ccggacgcag gcagatgtag
      961 agatccagtt cctggcgcag ggcaacgttc agagagcgaa taccgccacc aacaggagtg
     1021 gtcagcggac ctttaatggc aacgcgatat tcacgaatca gatcaagggt ttcagcaggc
     1081 agccagacat cctgaccata aacctgtgtg gatttttcac cggtgtaaat ttccatccag
     1141 gagattttac gctcgccttt ataggctttc tcgactgcag cgtcgaccac tttcagcatg
     1201 gctggggtta catctacacc gattccatca ccttcaatgt aagggataat cggattttca
     1261 ggaacgttga gtttgccgtt ttgcagggtg atcttcttgc cttgtgccgg aacaactact
     1321 ttactttcca ttcacctctc cttcgagcgc tactggtttg ctcgtctgcc accacgttgc
     1381 atatgcgttt gcgtcctgcg atacggatgc tttagagcaa ttttttgtta atgatttgta
     1441 attggcttgt caatgctacc ctattgtttg ccgtcatgaa agatgcgcgt tattaggcta
     1501 taatgcggca attcataatc tctgaaaata ccatgcaaaa aacttctttt agaaatcacc
     1561 aggttaagcg attcagctcg caacgttcta ctaggcgtaa acctgaaaac cagcccacgc
     1621 gtgtgatcct gttcaataaa ccctacgatg ttcttccgca gttcaccgat gaagccggac
     1681 gcaaaacatt aaaagaattc atcccggttc agggcgttta tgcagcaggt cgtcttgacc
     1741 gcgataccga agggttactg gtgctgacca ataacggcgc gttgcaggcg cgtttaaccc
     1801 agccgggcaa acgcaccggg aaaatctatt atgtgcaggt ggaaggtatt cccacacaag
     1861 acgcacttga agccttgcgc aatggcgtaa ccttaaatga tggccctacc ctgcccgccg
     1921 gcgcggagct ggttgacgaa ccagcgtggt tatggccgcg gaatccacca attcgtgaac
     1981 gcaaaagtat tcccaccagc tggctgaaga tcaccttata tgaaggacgt aatcgccagg
     2041 tgcgccgcat gaccgcccat gttggtttcc ccacgctgcg actgattcgt tatgcgatgg
     2101 gtgattacac tttggataac cttgccaatg gcgaatggcg agaagcaaca gaataaggat
     2161 gcacaatgtt taaaccgcac gttaccgttg cttgcgtagt gcacgcagag ggtaaatttt
     2221 tagtcgttga agagacgatt aatggtaaag cgttatggaa ccaacctgcc gggcatctgg
     2281 aagccgatga aaccttagtg gaagctgccg cccgtgagct atgggaagaa accggcatca
     2341 gcgcgcaacc gcaacacttt attcgtatgc atcagtggat tgcgccagat aaaacgccgt
     2401 ttttgcgttt cctctttgcc attgagcttg agcaaatatg cccgacccag cctcatgaca
     2461 gcgatatcga ctgctgtcgt tgggtcagcg ccgaagaaat tttaaaggcg tcaaatcttc
     2521 gttcgccgct ggtggcggaa agtatccgtt gttatcaaag tgggcaacgc tatccgctgg
     2581 agatgattgg cgattttaac tggcctttta caaagggtgt catctaaaag gctgcgtgat
     2641 agaatacgcc gccttgaagt tcaatgtcgt gagtgatcca atgtctgaaa ccgcaaaaaa
     2701 agtaatcgtc ggcatgtccg gcggtgtcga ttcctccgtt tctgcctggc tgttgcaaca
     2761 acagggatat caggttgaag gcctgtttat gaagaactgg gaagaagacg acggtgagga
     2821 atattgcaca gcggcagcgg atctggctga tgcccaggct gtctgcgaca agctcggcat
     2881 tgaactgcac accgttaact ttgctgccga gtactgggac aatgtcttcg aactgttcct
     2941 tgccgaatat aaagccggtc gcacgccgaa tccggatatt ctgtgcaaca aagagatcaa
     3001 atttaaagct ttcctcgaat ttgccgccga agatttaggt gccgattata tcgctaccgg
     3061 tcattacgta cgtcgggccg atgtcgatgg caagagccgc ctgctgcgtg gtctggacag
     3121 caataaagac cagagctact tcctttatac gctcagccat gagcagattg cgcaaagcct
     3181 gttcccggtc ggcgaactgg aaaaaccgca ggtgcgtaag attgctgaag atcttggtct
     3241 ggtcaccgcg aagaaaaaag actctaccgg catttgcttc attggcgaac gtaaattccg
     3301 cgagttcctg ggccgttatc tcccggggca accgggcaaa atcattaccg tcgatggcga
     3361 tgaaattggt gagcaccagg ggctgatgta tcacactctc ggtcagcgta aaggtctggg
     3421 tatcggtggc accaaagaag gcaccgaaga accttggtat gtggtggaca aagacgtcga
     3481 aaacaacatt ctggttgtcg ctcagggcca tgaacacccg cggctgatgt ctgtcgggtt
     3541 gattgcccag cagttgcact gggtcgatcg cgaaccattc accggcacta tgcgttgcac
     3601 ggtaaaaacc cgctatcgcc agaccgatat cccttgcacc gtcaaggcgc tggacgatga
     3661 tcgcattgaa gtgattttcg atgaaccggt tgccgccgtg acgccgggcc agtctgccgt
     3721 cttctataac ggtgaagtgt gcctcggtgg cggtattatt gagcagcgtc tgccgctgcc
     3781 ggtctgatta ttatctttac ttaaaacaag gaagcagtga acgtggcaaa gaattactat
     3841 gacatcaccc tcgccctggc cggtatttgt cagtcggcac gcctggtgca acaactcgct
     3901 caccaggggc attgtgatgc cgatgcgcta cacgtctcac tcaacagtat tattgatatg
     3961 aaccccagct cgacgctggc ggtttttggc ggtagcgaag ccaacctgcg cgtcgggctg
     4021 gaaaccctgc tcggcgtgct caatgccagc agtcgccagg gcttaaacgc cgaattaacc
     4081 cgctacacac tcagcttgat ggtgcttgag cgcaaactct cctcagcgaa aggcgcgctc
     4141 gacactctgg gcaaccggat caacggcctg caacgccagc tcgaacactt cgatttacag
     4201 tccgaaacgc tgatgagcgc gatggctgct atctatgttg atgtgatcag cccgcttggc
     4261 ccacgcattc aggtcaccgg ttcccctgct gtactgcaaa gcccacaagt gcaggcgaaa
     4321 gttcgcgcaa ccctgctggc aggcattcgc gccgccgtgc tctggcacca ggtcggcggc
     4381 ggacgtctgc aactgatgtt ttctcgtaat cgcctgacca ctcaggcaaa acaaattctt
     4441 gctcatttaa ccccggagtt gtgatctatg gaattatcct cactgaccgc cgtttcccct
     4501 gtcgatggac gctacggcga taaagtcagc gcgctgcgcg ggattttcag cgaatatggt
     4561 ttgctgaaat tccgtgtaca agttgaagta cgttggctgc aaaaactggc cgcgcacgca
     4621 gcgatcaagg aagttcctgc ttttgctgcc gacgcaatcg gttaccttga tgcaattgtc
     4681 gccaatttca gcgaagaaga tgccgcacgc atcaaaacca tcgagcgtac cactaaccac
     4741 gacgttaaag cggttgagta tttcctgaaa gaaaaagtgg cggagatccc ggaactgcac
     4801 gcggtttctg aattcatcca ctttgcctgt acttcggaag atatcaataa cctctcccac
     4861 gcattaatgc tgaaaaccgc gcgtgatgaa gtgatcctgc cgtactggcg tcaactgatt
     4921 gatggcatta aagatctcgc cgttcagtac cgcgatatcc cgctgctgtc tcgtacccac
     4981 ggtcagccag ccacgccgtc aaccatcggt aaagagatgg caaacgtcgc ctaccgtatg
     5041 gagcgccagt accgccagct taaccaggtg gagatcctcg gcaaaatcaa cggcgcggtc
     5101 ggtaactata acgcccacat cgccgcttac ccggaagttg actggcatca gttcagcgaa
     5161 gagttcgtca cctcgctggg tattcaatgg aacccgtaca ctacccagat tgaaccgcac
     5221 gactacatcg ccgagctgtt tgattgcgtt gcacgcttca acaccattct gatcgacttt
     5281 gatcgtgacg tctggggtta tatcgccctt aaccacttca aacagaaaac cattgctggt
     5341 gagattggtt cttccaccat gccgcataaa gttaacccga tcgacttcga gaactccgaa
     5401 gggaatctgg gcctttccaa cgcggtatta cagcatctgg caagcaaact gccggtttcc
     5461 cgctggcagc gtgacctgac cgactccacc gtgctgcgta acctcggcgt gggtatcggt
     5521 tatgcgctga ttgcgtatca atccaccctg aaaggcgtga gcaaactgga agtgaaccgt
     5581 gaccatctgc tggatgagct ggatcacaac tgggaagtgc tggctgagcc aatccagaca
     5641 gttatgcgtc gctatggcat cgaaaaaccg tacgagaagc tgaaagagct gactcgtggt
     5701 aagcgcgttg acgccgaagg catgaagcag tttatcgacg gtctggcgtt gccagaagaa
     5761 gagaaagccc gcctgaaagc gatgacgccg gcaaactaca ttggtcgcgc catcaccatg
     5821 gttgatgagc tgaaataaac tcgtatcagt gccggatggc gatgctgtcc ggcctgctta
     5881 ttaagattat ccgcttttta ttttttcact ttacctcccc tccccgctgg tttatttaat
     5941 gtttaccccc ataaccacat aatcgcgtta cactatttta ataattaaga cagggagaaa
     6001 taaaaatgcg cgtactggtt gttgaagaca atgcgttgtt acgtcaccac cttaaagttc
     6061 agattcagga tgctggtcat caggtcgatg atgcagaaga tgccaaagaa gccgattatt
     6121 atctcaatga acatttaccg gatattgcga ttgtcgatct cggattgcca gacgaggacg
     6181 gtctgtcact gattcgccgc tggcgtagca atgatgtttc actgccgatt ctggtattaa
     6241 ccgcccgtga aagctggcag gacaaagtcg aagtattaag tgctggtgct gatgattacg
     6301 tgactaaacc atttcatatt gaagaggtga tggcgcgaat gcaggcatta atgcggcgta
     6361 atagcggtct ggcttcacag gtcatttcgc taccaccgtt tcaggttgat ctctctcgcc
     6421 gtgaattatc tattaatgac gaagtgatca aactgaccgc gttcgaatac accattatgg
     6481 aaacgttgat acgcaataat ggcaaagtgg tgagcaaaga ttcgttaatg ctccaactct
     6541 atccggatgc ggagctgcgg gaaagccata ccattgatgt actgatggga cgtctgcgca
     6601 aaaaaattca ggcacaatat ccgcaagaag tgattactac cgttcgcggc cagggctatt
     6661 tgttcgaatt gcgctgatga aaaaattact gcgtcttttt ttcccgctct cgctgcgggt
     6721 acgttttctg ttggcaacgg ctgcggtagt actggtgctt tcgcttgcct acggaatggt
     6781 cgcgctgatc ggttatagcg tcagtttcga taaaactacg tttcggctgt tacgtggcga
     6841 gagcaatctg ttctataccc ttgcgaagtg ggaaaacaat aagttgcatg tcgagttacc
     6901 cgaaaatatc gacaagcaaa gccccaccat gacgctaatt tatgatgaga acgggcagct
     6961 tttatgggcg caacgtgacg tgccctggct gatgaagatg atccagcctg actggctgaa
     7021 atcgaatggt tttcatgaaa ttgaagcgga tgttaacgat accagcctct tgctgagtgg
     7081 agatcattcg atacagcaac agttgcagga agtgcgggaa gatgatgacg acgcggagat
     7141 gacccactcg gtggcagtaa acgtctaccc ggcaacatcg cggatgccaa aattaaccat
     7201 tgtggtggtg gataccattc cggtggagct aaaaagttcc tatatggtct ggagctggtt
     7261 tatctatgtg ctctcagcca atctgctgtt agtgatcccg ctgctgtggg tcgccgcctg
     7321 gtggagttta cgccccatcg aagccctggc aaaagaagtc cgcgaactgg aagaacataa
     7381 ccgcgaattg ctcaatccag ccacaacgcg agaactgacc agtctggtac gaaacctgaa
     7441 ccgattgtta aaaagtgaac gcgaacgtta cgacaaatac cgtacgacgc tcaccgacct
     7501 gacccatagt ctgaaaacgc cactggcggt gctgcaaagt acgctgcgtt ctctgcgtag
     7561 tgaaaagatg agcgtcagtg atgctgagcc ggtaatgctg gagcaaatca gccgcatttc
     7621 acagcaaatt ggctactacc tgcatcgtgc cagtatgcgc ggcgggacat tgctcagccg
     7681 cgagctgcat ccggtcgccc cactgctgga caatctcacc tcagcgctga acaaagtgta
     7741 tcaacgcaaa ggggtcaata tctctctcga tatttcgcca gagatcagct ttgtcggtga
     7801 gcagaacgat tttgtcgagg tgatgggcaa cgtgctggat aatgcctgta aatattgcct
     7861 cgagtttgtc gaaatttctg caaggcaaac cgacgagcat ctctatattg tggtcgagga
     7921 tgatggcccc ggtattccat taagcaagcg agaggtcatt ttcgaccgtg gtcaacgggt
     7981 tgatacttta cgccctgggc aaggtgtagg gctggcggta gcccgcgaaa tcaccgagca
     8041 atatgagggt aaaatcgtcg ccggagagag catgctgggc ggtgcgcgga tggaggtgat
     8101 ttttggtcgc cagcattctg cgccgaaaga tgaataaata tgtccatact tcacgcatta
     8161 cgttaagcat ccgttataat cggttgcaga taccagcctg tggatgctta acatggaata
     8221 ccaactcact cttaactggc ccgattttct tgaacgtcac tggcagaaac gcccggtggt
     8281 gttaaaacgc ggctttaata attttattga cccgatctct ccagacgagt tggcgggtct
     8341 ggcgatggaa agcgaagttg acagtcgact ggtcagtcac caggatggca aatggcaggt
     8401 cagccacggc ccgttcgaaa gctacgatca tctcggtgaa accaactggt cattactggt
     8461 acaggcagtg aaccactggc atgagccgac cgccgcgctg atgcgaccgt tccgtgaact
     8521 accggactgg cgtattgatg atctgatgat ttctttttct gtacctggcg gcggcgtcgg
     8581 cccgcatctc gatcagtacg acgtgtttat cattcagggt accggacgtc gtcgctggcg
     8641 agtgggcgaa aagctgcaaa tgaaacagca ctgcccacat ccggatctgt tacaggtcga
     8701 tccgttcgaa gccatcatcg atgaagagct ggagcctggt gatattcttt atattccgcc
     8761 aggattcccg catgaaggct acgcgctgga aaatgcgatg aactattccg tgggctttcg
     8821 cgcgccaaat acgcgggaac tgattagtgg atttgccgat tatgtgctgc aacgtgaact
     8881 gggcggcaac tactacagcg atcctgatgt tccacctcgc gctcatcctg cggatgttct
     8941 gcctcaagag atggataaac tgcgtgagat gatgctcgaa ttgatcaacc agccggaaca
     9001 ctttaagcaa tggtttggcg agtttatatc ccagtcacgt catgaactgg atatcgcgcc
     9061 gccagaaccg ccttatcagc cagatgaaat ctacgatgcg ctgaaacaag gtgatgtgct
     9121 ggtgcgcctg ggtggtctgc gcgtattgcg cattggcgac gacgtgtatg ccaatggtga
     9181 gaagatcgat tccccgcacc gtccggcact ggatgcactc gccagcaaca ttgcgctgac
     9241 tgcggagaat tttggcgatg cgctggaaga tccgtcattc ctcgcgatgc tcgcggcgct
     9301 ggtcaatagc gggtattggt tcttcgaagg gtaagtttgg atttaagccg gatgcggcat
     9361 cacatgccgc atcccttttc gcttacttcc gttgcgccgt taactcggca atacggacga
     9421 tcacctgcac cgctttttcc ataccttcca gagtcacaaa ctcatgctta ccatgatagt
     9481 tgtaaccgcc agtgaacaag ttcgggcacg gtaatcccat aaacgacaac tgcgcgccgt
     9541 cggtaccacc gcggatcggt ttcagttccg gttcaatatc gcagtcacgc atcgcctgct
     9601 gggcgatatc gagaatatgc ggatgctcaa ccactttctc tcgcatattg tagtaactgt
     9661 cttcaatcac cagttcaatg tagcaatcag gatgtaaccc tttgcccact tttttggcga
     9721 tctccatcat tttacgttta cgcgcttcaa actgtttacg gtcgaaatca cggatgatgt
     9781 agtgcatatc ggcccgttca acggtgcctt tcatgctcgc caggtgatag aaaccttcat
     9841 agccttctgt catttccggg ctttcatccg ccggaacttc cgcatgaata cgtgccgcca
     9901 gcgacagcgc atttaccatc actcctttcg ccgtgcccgg atgaacattg ttaccgacaa
     9961 ttttgatatt gaccgatgcg gcgttgaagt tttcaaactc cagttcgcct acgccaccac
    10021 cgtcaacagt gtaagcccaa cgggcatcga aggcatcaac atcaaaatgt ttcgcccctt
    10081 tgcccacttc ttcatccggg gtaaaggcga cgcgaatatc accatgcgga atgtttttct
    10141 gttgcaatac cgccagcgcg gtcatgattt ctgcaatacc tgctttgtca tcggcaccta
    10201 acaaggtttt accatcggtg gtaatcaacg tctgacccag tagctgatgc agcaccggga
    10261 acataaccgg tgataaaact tcatcgccga tacccagcgc aatatcgcca ccgcgatagt
    10321 tttcaacaat ttgcggatta acatttttgc cgctgcaatc cggtgaggta tccacatgag
    10381 aaataaagcc aatcgccggg atatcgccag ggacgttagc cggtaacgtc gccatcaaag
    10441 tgcccttctc acttaaggtc acattgataa gccccatttc ttcgagctgc tctttcagca
    10501 gatgcaataa cttccattgg ccttccgtgc tgggaacctg tctcacccct gcttttgatt
    10561 gggtatccag agatacgtag ttcaaaaatc gctcaagtag tttatccatg tagtcaccct
    10621 cactttttgt gacaacatta ttaagaagca agaaaagaca aatattgcgt caggtcactt
    10681 ttacccctgc aagcgggaat atttatcagc attaccttaa tgaatataaa gctaagccag
    10741 taattcacaa caaagattgg cgattcaagc ggtttgccgt agaattaccg ccctcattaa
    10801 gagtcaccaa ggtggttaac cacaaacccc gcatcggtaa gccatccgtt gcgtttacat
    10861 gggacagagt aaaaaattga ataaacaacc gagttcgctt tcaccgctgg tgcaattggc
    10921 gggaattcgc aaatgctttg atggtaaaga ggtcattccc cagctggatc tgactatcaa
    10981 caatggcgag ttcctcacgc tgcttggccc ttctggctgc ggtaaaacaa ccgttcttcg
    11041 cctgattgca ggtctggaaa ctgttgattc cggacgcatc atgctggata acgaggacat
    11101 cacccacgtt ccggcggaaa accgctatgt gaacactgtt ttccaaagct acgcactttt
    11161 cccccacatg accgtgttcg aaaatgtggc ctttgggttg cgcatgcaaa aaacccccgc
    11221 tgctgaaatt acgccccgcg tgatggaagc cctgcggatg gtgcaactgg aaaccttcgc
    11281 tcaacgtaaa ccgcatcagc tctctggtgg tcaacagcaa cgcgtcgcca ttgctcgcgc
    11341 ggtggttaac aagcctcgtc tgttgttgct ggatgagtcg ctctcagcgc tggattacaa
    11401 actgcgtaag caaatgcaga acgagctgaa agcgttacag cgtaagcttg gtattacctt
    11461 cgtctttgtc actcacgatc aggaagaagc gctcaccatg tcagacagga ttgtggtgat
    11521 gcgcgagggt cgtattgagc aagatggtac accgcgcgaa atttacgaag agccgaaaaa
    11581 cctgtttgtt gccggcttca ttggcgaaat caatatgttt aacgccactg tcatcgaacg
    11641 tctcgacgag cagcgcgtac gcgccaacgt tgaaggccgc gaatgtaata tctacgttaa
    11701 cttcgccgtt gaaccggggc aaaaactgca tgttctgctg cgcccggaag acttacgtgt
    11761 tgaagagatt aacgacgaca accacgctga agggctgatt ggttacgttc gtgagcgtaa
    11821 ctacaaaggc atgacgctgg agtcggttgt tgaactggaa aatggcaaga tggtgatggt
    11881 cagcgaattc ttcaatgaag acgaccctga ctttgaccac tctctcgacc aaaaaatggc
    11941 cattaattgg gtagaaagct gggaggtcgt actggctgat gaagaacaca agtaagttcc
    12001 agaatgtagt gattgtcact attgtcggtt ggcttgtgtt gtttgtcttt ctgcccaacc
    12061 tgatgatcat tggcaccagc tttttgaccc gcgacgacgc cagtttcgtc aaaatggtct
    12121 ttacgctgga taactacacg cgtctgctcg atccgctcta ttttgaagtg ctattgcact
    12181 cgctgaatat ggcgctgatc gccaccctcg cctgcctggt gctgggctac ccgtttgcct
    12241 ggtttctggc gaagctgcca cacaaggtgc gtccgctgct gctgtttctg ctgattgttc
    12301 ccttctggac caactcactg attcgcatct acgggctgaa aattttcctc agcaccaaag
    12361 gctatctcaa cgagtttttg ctctggctgg gcgttatcga cacaccaatt cgtatcatgt
    12421 tcacgcccag tgcggtgatt atcggtctgg tttacattct gctaccgttt atggtgatgc
    12481 cgctgtactc cagtatcgaa aaactggata agccgttgct tgaggcggcg cgcgatctcg
    12541 gtgccagcaa gttacagact tttatccgta tcattattcc actgacgatg ccgggaatta
    12601 ttgccggatg tctgctggtg atgctgccag cgatgggcct gttctatgta tccgacctga
    12661 tgggcggtgc gaaaaacctg ctgatcggta acgtcatcaa ggtccagttc ctcaatattc
    12721 gtgactggcc gtttggtgca gccaccagca ttacgctgac tatcgtaatg ggcctgatgt
    12781 tgctggttta ctggcgcgct tctcgtctgc tgaataaaaa ggtggaactc gaatgatcgg
    12841 tcgactgctt cgcggcggtt ttatgaccgc tatctacgcg tacctgtata tcccaatcat
    12901 tattttgatt gtgaactcct ttaacagctc gcgctttggc atcaactggc agggttttac
    12961 caccaaatgg tatagcctgc tgatgaacaa cgacagcctg ttacaggcag cgcagcattc
    13021 actaacaatg gcggtgtttt cggcgacatt tgctacgctt atcggttcac tgacggcagt
    13081 tgcgctgtac cgttatcgct ttcgtggtaa gccgttcgtt agcggaatgc tgtttgtggt
    13141 gatgatgtca ccagatatcg tgatggcgat ttctctgctg gtgctgttta tgctgctggg
    13201 tattcagctt ggcttctggt cgctgctgtt ctcgcatatc accttctgcc tgccgtttgt
    13261 ggtggtgacg gtgtattcgc gcctgaaagg ttttgacgtg cggatgctgg aagcggcgaa
    13321 agatctcggt gccagtgaat ttaccattct gcgaaaaatc attctgccac tggcaatgcc
    13381 agcggtggcg gcgggctggg tgttaagctt taccctgtcg atggacgatg tggtagtttc
    13441 ttcgttcgtc accggaccaa gttatgaaat tctgccgtta aaaatttatt cgatggtcaa
    13501 agtcggcgta tcgccggaag ttaacgcgct ggcaaccata ttactggtgc tgtcgctggt
    13561 gatggttatt gccagccagc ttattgctcg tgataaaacg aaaggtaaca caggggacgt
    13621 taaatgaaaa aatggtcacg ccacctgctc gcggcgggtg ctctggcact gggcatgagc
    13681 gccgctcacg ccgatgacaa caacacgctg tatttctaca actggaccga gtacgtgccg
    13741 ccaggactgc ttgaacagtt caccaaagaa accggtatta aggctatcta ttcgacttac
    13801 gagtcgaacg aaaccatgta cgccaagctg aaaacgtaca aagacggtgc ctatgacctg
    13861 gtggttcctt caacctatta cgtcgataaa atgcgtaaag aagggatgat ccagaagatc
    13921 gacaagtcga agttaacgaa cttcagcaat ctcgatccag acatgctcaa caagccgttt
    13981 gacccgaata acgactactc cattccgtat atctggggtg cgacggcgat tggcgttaac
    14041 ggtgatgcgg tggatcctaa atctgtcacc agttgggcag atctgtggaa gccagagtac
    14101 aaaggtagcc tgctgctgac cgacgatgcc cgtgaagtgt tccagatggc gctgcgcaaa
    14161 ctgggctact ccggtaacac caccgatccg aaagagattg aagctgcata taacgagctg
    14221 aaaaaactga tgccaaacgt cgcagcgttt aactccgata acccggctaa cccgtacatg
    14281 gaaggcgaag ttaacctcgg catgatctgg aacggctctg ctttcgttgc acgccaggcg
    14341 ggtacgccaa ttgacgtggt gtggccgaaa gaaggcggca ttttctggat ggacagcctg
    14401 gcgatcccgg caaatgccaa aaacaaagaa ggcgcgctga aattgatcaa cttcctgctg
    14461 cgcccggatg tggcaaaaca ggttgccgaa actatcggtt atccaacgcc aaaccttgcg
    14521 gcgcgtaagc tgttaagccc ggaagtggcg aacgataaaa cgctctaccc ggatgctgaa
    14581 accattaaaa atggtgaatg gcagaatgac gttggcgcag ccagcagcat ttatgaagag
    14641 tattatcaga agctgaaagc aggacgttaa ttcagcaaac gggcggtggt attaccgccc
    14701 gttttttagt tagcaaggat gtggctaatg agtcaggaca gtaaagtttt atcccgctct
    14761 ttcgtcggta ttgtggtccc cttgtaggcc tgataagcgt agcgcatcag gcaatgcttc
    14821 ctgctttata atcccttcag caacttttca acaaactcag gcaccacctg gcttgccggg
    14881 ccgtaatatt tctcggcaaa ttcattaccg acctgacttg gttcgagatt cagttccacg
    14941 gtgtgcgcgc catgcagttt cgcttcgtga acaaacccag ccgccggata aacatgcccg
    15001 gaagtaccaa tggcaatgaa aatatcagcc atcgacaacg ccatataaat ttcatccatg
    15061 ccgagtggca tttcgccaaa ccaaacgacg tgcgggcgca agggtgccgg gaactggcaa
    15121 caatggcatt tatcttctgg cgtaacgtct ccggtccagt cgagaacctg accactttgc
    15181 gaacaacgaa ctttcagcaa ttccccatgc atatgaatga cattggtatt acctgcgcgt
    15241 tcatgcaggt tgtctatatt ctgcgttacc agcaaaaagc gatcgccgag ggcgtcttgc
    15301 agtttagcca gcgcaagatg cgcggcgtta ggctgaattt ctggctgctg cagctgtcga
    15361 cgacgggcgt tataaaacgc ttgcaccagt tcaggatcgc gatcgaaacc ttccggcgtt
    15421 gccacatctt caacccgatg ttcttcccac aggccatctg cggcgcgaaa ggtacgaata
    15481 cctgattccg cagaaattcc tgcccctgtc agtacgagta ctcttggttt ttccattgct
    15541 tccggcacca ctttatctct gaaaaaaata cgctgacgca aacgctcgcg caggcggcgt
    15601 ttatttttac gaaaacgact taaccgatga ccccgacgcg acagcatagc aacctctgtt
    15661 gtttaatcgg ttagatgtag gaaggccgca ccacgcattc ctcccgcatc accgtggcgc
    15721 gcgcgttcaa tgcgcggaac acgagctaca ggtaagagat gacgaggcag cctgtccgcc
    15781 agttgcgttg tgattgccgg gaaattcgat aagccaccac caatgacgac caggtcagag
    15841 tcaacgatgg tcaggatatt tcccagacaa accgctaata aatccagata acgctcaacg
    15901 tgcgcccttg cctgctcatc gccttgatca taaagcgcaa taatttcggg agcctgcaac
    15961 ggttgatgat aatagtgttg ccagagccac gcaaagcccc gacctgatag ataattttcg
    16021 atacaaccaa tttgaccaca gccgcagcgg cgtaacggga aatccagccc catcatggtt
    16081 aacgcatcaa ccggcagacg catatggcca aactcgccgg taatgtagct tttaccggta
    16141 atcggtttac cgttgaaaat cagcccgccg ccaacgccgg tgccgagaat taaccccatc
    16201 accagtggat attgcgtaaa ttcatcatcc caggcttctg aaagggcaaa acagttggca
    16261 tcgttatcaa ggcgtacatc gcgatcaaga cgtgcgctca ggtcggcacg cagcggttta
    16321 ccgctggcag cggggacatt ggcggcatac agcgtaccat cttctgtttc cggcattccc
    16381 ggaataccga tgccaacaga acctttacag ccaaaacgtt ggtcagcttc ggctaccagc
    16441 tcgcatactg catctaaaaa tgcgtcatag ctgtcacgcg gtgtcggcac ccgcttttcc
    16501 cactgcaact gccgaccgct atcaaacacg ccaagcgcaa tttttgttcc accaatatca
    16561 aacccgtaat acattgccgc tccttgtttt aatgtactgc ctttactggc cgctaaggac
    16621 tcgcgcaggg tcaatattac tggcgcgccg cgccggatac caacttgcca aaagactcag
    16681 caacaatgct gtgaccagta cgtagaagac gtccagccag tgcaattccg atggcaggaa
    16741 gtcaataaaa tagatatcgc tggagaggaa ctgatgaccg atcagctttt caatccgctc
    16801 aataatcggg gtaagttgca gtgaaacaac tacgccgata atcacaccac acaggctacc
    16861 gaacagccct gccagcaatc cataccagac aaagatggcg cgaattaaac catctttcgc
    16921 ccccagcgtt cttaatactg cgatatcgcc actcttgtct ttcaccgcca tcactaaggt
    16981 ggagacgatg ttgaaacagg ccacgccaat caccagtacc atcgccagat acataatggc
    17041 gcggatcatc tggatatcgc gatacatata gccgtaagta ccaatccaac ttttaatata
    17101 aacatagctg ttggtcactt cacccgcatc gcgtaccagc ttattggcgt tgaaaacatc
    17161 cgtcatttta agggcaatac ctgacacgct ggaacccata tcaagatatt gttgggcatc
    17221 cgccagcggg atcatggcaa aactgtgatc gagttgacca ctcaactgca aaataccggc
    17281 aatgtgcaaa cgcacacgtt ttggctgcat cagtttatgc tcgggattcg agttgggaat
    17341 catgatcgac acccaatcgc cctgcttcac tttcagcgca tccgccacgc ctttgccgat
    17401 gataatttgc tgttcgcccg ctttaaaatt gcgccaggca tccccctgaa caaacgaggg
    17461 taatgcgctc agacgctgtt cctgttgcgg gttaacgccc ttcacctgaa ttgcgcgcag
    17521 attggtacca ctttctacca gtccggtgaa attgatatac ggcgcagcgg cggcaatacc
    17581 cggcactttt tgcacgttat ccagtgcttc ctgccagtta gtccatggct gatttaccgc
    17641 ctcaatttca ccgtgcggta ccacagcaag aatgcggtta ttcagttcgc gttcaaagcc
    17701 gttcatcgcg cttaagccga cgatcaatac cgccacgccg agggcaatgc caatggtaga
    17761 aatgacagag attagcgaca ccatgccgcc gcgtcgccgt ccgcgactaa aacgcaggcc
    17821 aatcaataac gataaaggca tcgccattac tccgccccca tcaggctcag ttccgccgtc
    17881 agacgtccat cacgcatttc cagttggcgg ctcatacgtt tcgccagttg caggtcgtga
    17941 gtaaccacca ggaaggcggt gccctgcaag cgattcaatt ccccaagcaa ctggaagatg
    18001 ctgtcggcgt tacgcgcatc gaggttaccg gtaggctcat ccgcaagtac cagacgcgga
    18061 ttattgacca gcgcacgggc aatcgccaca cgctggcgtt cgccgccaga aagttcagat
    18121 gggcggtgat tcgcacgatg ctccagcccc accgctttta acatctccag cgcacggctg
    18181 ttgatttcag cgggcttttt cttgccaatc agcagtggca tagccacgtt ttccagggca
    18241 gtaaaatccg gcagcaggtg gtgaaactga taaataaagc ccagcttctg gttgcgcagt
    18301 tctgctttcg ccgccgaaga cagtttgctc atcggttgac cgttaaagat cacatcgccg
    18361 gaggttggtg tatccagccc gcccagcagg tgcagcaagg tacttttacc ggaaccagag
    18421 ctaccgacga tcgccatcat ttcaccttcg ccgacgctga aactgacatt gtgcagtaca
    18481 tcggtttgca cactgccttc ctgatagcgt ttgcacaggt tgtcgcattg caacaggatc
    18541 ttattcataa cgtaaagcct cagcgggttg agtggcggca gcgcgccatg aagggtaaag
    18601 cgtagacagc agcgcgatag ccatcgccac cagcgcaata acaatgacct gtaaaggttc
    18661 gatagcgacc ggcagcgccg cgccatcaag caggacgccg attatcggca tcagattatt
    18721 taactggctg gcaagcagtg cgccaagcgc cgctccgagg atcgcaccga taatcccggc
    18781 gctggcccct tgcaccataa agaccatcat gatttgtcgc ggagttaagc cttgcgtttg
    18841 caggatcgcg acttcgccct gcttctccat taccatcagc cctagtgagg taataatgtt
    18901 aaacgccgca acggcgacaa tcaggctcag cagcaagccc atcatatttt tttccatgcg
    18961 tacggcctgg aacagctcgc ctttacggtc acgccagtcc tgccatttgc tgccttcagg
    19021 cagtttttgc tgacttaaag agtcaacttt cagcggctca tccagccaca aacgccagcc
    19081 ggtaatattg cctgccggat aacgcatcag acgcgaggca tcctcaatat tcaccagcat
    19141 ttcatagcca tcgacttcac tgttggcggc gaaattgcca atcacattga acaggcgctg
    19201 gcttggaata cgccccatcg gcgtgaactg gctggcagat ggtaccatca cgcggatttg
    19261 atcaccgcga ttaacgccta gctgtgaggc aagttgttcg ccgaggatga cattatattt
    19321 ccccggctcg aggtcagttt gtttcacatt gaccagatac ggcgtaagtg gatctttttg
    19381 cgccgggtcg ataccgagca tcaccccgac cgccacgctg cgcgcgcttt gcagtaccac
    19441 atcaccggta gtaataggtg cgacgcgatt aacgccgtcc agtttgactg ccgtttccgg
    19501 gagttgctgc gggttaagag agccatgctc agaagagaga attgcctgtg gcatcaggcc
    19561 aaggatgttg ttttgcagct cgcgctcaaa gccgttcatc actgacaata ctgtgaccag
    19621 cgccatcacc ccgagggtaa tgccgatggt agaaagccag gagacgaaac gaccgaagcg
    19681 atccgctgca cgcccacgca tgtaacgcag gccaataaat agagcgacag gttggtacat
    19741 gaaatccgtc tggttgctgt agcaaagaca cgagtatata agcgaaagca agatgggtaa
    19801 acgtctgaac tgtaagtgat cgtcagggct aatctgaaaa gcccccgtaa atcaatcggc
    19861 taaattgcca tgttttatca tcaatcttgc cgcctgatct taatctgaaa aatcatccaa
    19921 tacagacaga tacccattac acctcggctc ataacccgca ggatcaaccg catgtaaatt
    19981 aaaaaagagc gtgttgtcct gatgaaacaa aaagagctat ggattaacca gatcaaaggg
    20041 ttatgtattt gtctggtggt gatttatcac tcggtcatta ccttttatcc gcatatgacc
    20101 acttttcagc atccgttatc ggaagtcctg agtaaatgct ggatctattt caatctttac
    20161 cttgcaccct ttcgtatgcc ggtttttttc tttatatccg gctatttgat tcgccgctat
    20221 atcgacagcg taccgtgggg aaattgtctc gataaacgga tctggaacat cttctgggtg
    20281 ctggcgcttt ggggcgtggt gcagtggctg gcgttaagtg cgctgaacca gtggctggca
    20341 cctgagcgcg atttaagcaa tgcctccaat gccgcttatg ccgattccac cggtgagttc
    20401 ctgcacggga tgatcaccgc cagcaccagc ttgtggtatc tgtatgcgtt aattgtctat
    20461 ttcgtggtat gtaaaatttt tagccgtctg gcgctgccac tattcgcctt gtttgtactg
    20521 ctgagtgtgg cggttaattt cgttcccacg ccgtggtggg gaatgaacag tgtgatccgc
    20581 aatttgcctt attacagcct tggcgcatgg tttggcgcaa caataatgac ctgtgttaaa
    20641 gaggtgccgt tgcgccgcca tctgctgatg gcttctttgc ttaccgttct ggcggtcggt
    20701 gcctggttgt ttactatctc gctgctgttg tcgctggtat cgattgtggt gatcatgaag
    20761 ctgttttatc agtacgagca acgattcggt atgcgctcca ccagcctgct gaatgtgatt
    20821 ggctccaaca ccattgctat ctacaccacc catcgcattc tggttgaaat attcagctta
    20881 actctgcttg cgcaaatgaa cgcagcacgc tggtcgccgc aagtcgaact gacactcctg
    20941 ctggtttatc cctttgtcag tttgttcatc tgtactgttg cgggcttgct ggtaagaaaa
    21001 ctttcacagc gtgcattcag cgatctgttg ttctccccgc cttcactgcc cgctgccgtc
    21061 agttactctc gttaaaccat cgccccagcc ggggcgattc aatttgctaa accatgtcga
    21121 ttacggataa tgaacagcat tgcttatcag agatatgccc ccatatgttg aggcatatcc
    21181 taacgagaat ctgacaaccg ttatgcctga acaatatcgt tatacactgc ccgtcaaagc
    21241 gggtgagcag cgtctgctgg gcgagttaac cggcgcagcc tgtgcaacgc tggtagcgga
    21301 aattgccgaa cgtcacgccg gtccggtggt actcattgca ccagatatgc aaaatgctct
    21361 gcgtttgcat gatgaaatca gccagttcac cgatcagatg gtgatgaatc tggcggactg
    21421 ggaaactctt ccctacgaca gtttttcgcc tcatcaggac attatctcct cgcgcctttc
    21481 caccctttac cagctaccga cgatgcagcg tggcgtactg attgttccgg tgaatacgct
    21541 tatgcagcgc gtttgcccac acagttttct ccacggtcat gcgctggtga tgaaaaaagg
    21601 tcagcgcctg tcacgagatg cattacgaac ccaactggac agcgccggtt atcgccatgt
    21661 tgaccaggtg atggagcacg gcgaatacgc cacgcgcggc gcgttgctgg atctcttccc
    21721 gatggggagt gagctgcctt atcgtcttga tttctttgat gatgaaatcg acagcctgcg
    21781 ggtgtttgac gtcgacagcc agcgcacgct ggaggaagta gaagcgatca atctgctgcc
    21841 cgcgcacgaa tttccgaccg ataaagcggc aattgaactg ttccgcagcc agtggcgcga
    21901 taccttcgaa gtgaagcgcg atccggaaca tatttaccag caagtgagta aaggcacatt
    21961 acctgccggg atcgagtact ggcagccatt gttcttcagc gaaccactgc cgccgctgtt
    22021 cagttatttc cctgccaata ccttgctcgt gaatactggc gatctggaaa ccagtgccga
    22081 acgtttccag gctgacacgc tggcgcgttt tgagaatcgc ggcgtcgatc cgatgcgccc
    22141 gctgttgcca ccacaatcgc tctggctgcg ggtggacgag ctcttctcag agctgaaaaa
    22201 ctggccccgg gtgcagctaa aaactgaaca tttaccgaca aaagccgcga atgccaattt
    22261 aggtttccag aaactgccag acctggccgt tcaggcacaa caaaaagcgc cgctggatgc
    22321 gctgcgtaag ttcctcgaga ctttcgacgg tccggtggtg ttctcggtag aaagtgaagg
    22381 tcgccgtgaa gcgctgggtg aactgctcgc acgaattaaa attgctccgc aacgcattat
    22441 gcgtcttgat gaagccagcg accgtgggcg ttatctgatg attggcgctg ccgaacatgg
    22501 ttttgtcgat aaggtgcgta atctggcgct gatctgcgaa agcgatctgc tcggtgaacg
    22561 tgttgcccgc cgtcgtcagg attctcgccg caccatcaac cccgatacac tgatccgtaa
    22621 ccttgcggag ctgcatattg gtcagccggt ggtccatctg gagcacggtg tcgggcgcta
    22681 tgccggaatg accacgctcg aagcgggcgg cattactggc gagtatttga tgctcaccta
    22741 tgccaacgac gccaaactgt atgttccggt gtcgtcactg catctgatta gccgttacgc
    22801 aggtggcgcg gaagaaaacg ccccgctgca taaacttggc ggcgatgcgt ggtcacgcgc
    22861 gcggcagaaa gcggcggaaa aagtgcgtga tgtggcggcg gaattgctgg atatctacgc
    22921 gcaacgcgcc gccaaagagg gcttcgcgtt taaacacgat cgtgagcagt atcagttgtt
    22981 ctgcgacagc ttcccgtttg aaaccacgcc ggatcaggcg caggccatta atgcggtact
    23041 tagcgacatg tgtcagccgc tggcaatgga tcgtctggtg tgcggcgatg ttggctttgg
    23101 taaaacagaa gtggcgatgc gcgccgcttt cctggcagta gataaccaca agcaggtggc
    23161 ggtgttggtg cctaccaccc ttctcgcgca gcagcattac gacaacttcc gcgaccgttt
    23221 cgccaactgg ccggtacgta tcgaaatgat ctcccgtttc cgcagcgcca aagagcagac
    23281 gcaaatcctt gcggaagtgg cggaagggaa aatcgatatt ctgatcggta cgcacaaact
    23341 gctgcaaagt gacgtcaagt ttaaagattt aggcctgctg attgtcgatg aagaacaccg
    23401 cttcggggtg cgtcataaag agcgcattaa agcgatgcgc gcgaacgtgg atattctgac
    23461 gcttactgca acgccgatcc cacgcacgct gaatatggca atgagcggaa tgcgtgacct
    23521 gtcgattatc gccacgccgc ccgcccgtcg tctggcagtt aaaacctttg tccgtgagta
    23581 tgacagcctg gtggtccggg aggcgatcct gcgtgaaatt ttgcgcgggg ggcaggttta
    23641 ttatctctac aatgatgtgg aaaacatcca gaaagctgcc gaacggctgg cagaactggt
    23701 gccagaagcg cggatcgcca tcggtcacgg gcagatgcgc gagcgcgaac tggaacgggt
    23761 gatgaatgat ttccatcatc aacgtttcaa cgtgctggtt tgtacaacca ttatcgaaac
    23821 cgggatcgac atcccgacag ccaacaccat tatcattgaa cgcgcggatc acttcggtct
    23881 ggcgcagctg caccagttac gcggtcgcgt cggacgttcg catcatcagg catatgcatg
    23941 gttgctgaca ccgcatccaa aagcgatgac taccgatgca caaaaacgtc ttgaagcgat
    24001 tgcctcgctg gaagatctcg gggcaggttt tgcgctggca acgcacgatc tggagattcg
    24061 cggcgcgggt gaactgcttg gcgaagaaca aagcggctca atggaaacca tcggtttctc
    24121 gctgtatatg gagttgctgg aaaacgccgt cgatgcactg aaagccggac gcgagccgtc
    24181 gctggaagat ctcaccagcc agcaaacaga agtcgagctg cggatgccgt cgctattgcc
    24241 agatgatttc attcctgacg tgaatacgcg tttgtcgttc tataaacgta ttgccagcgc
    24301 caaaacggaa aacgaactgg aagagatcaa agtcgagctt atcgatcgct tcggcctgct
    24361 gccagatccg gcgcgtaccc tgctggatat tgcccgtctg cgccagcaag cgcagaaact
    24421 ggggatcagg aagctggaag gtaatgagaa aggcggggtg atcgaatttg ccgagaagaa
    24481 tcacgttaat ccggcctggt tgattggttt gctgcaaaaa cagccgcagc attaccgcct
    24541 tgatggtccg acgcgcctga aatttattca ggatttgagt gagcggaaaa cgcgtatcga
    24601 atgggtacgc cagtttatgc gtgaactgga agagaacgcg atcgcttaat ggtaaatcgg
    24661 gggcgtttct gcgcccccat ctgcaacatt tacaaattct ttgcacttcc ctgcactatc
    24721 cgacactgtc accatccata attcaggctt atctgtttat tacaataacc ttatatttat
    24781 tattgatttt tggtgatgat caaaacgcgt ttttctcgct ggctaacgtt ttttacgttc
    24841 gccgctgccg tggcgctacc ggcaaaagcc aacacctagc cgctgccgcc agcgagcagt
    24901 cgtctggttg gcgaaaacaa atttcatgtg gtggaaaatg acggtggttc tctggaagcc
    24961 atcgccaaaa aatataacgt cggctttctc gctctgttac aggctaaccc cggcgttgat
    25021 ccttacgtac cgcgcgcggg cagcgtgtta acgatcccgt tgcaaaccct acttccagac
    25081 gcgccgcgcg aaggcattgt gatcaacatt gcggagctgc gtctctatta ctacccgccg
    25141 ggtaaaaatt cggtaaccgt gtatccaata ggtattggtc agttaggtgg tgacacgctg
    25201 acaccgacaa tggtgaccac cgtttcagac aaacgtgcaa acccaacctg gacgccaacg
    25261 gcaaacatcc gcgcccgtta taaagcacag ggaattgagt tgcctacggt agtgccggct
    25321 ggaccggata acccaatggg ccatcatgcg attcgtctgg cggcctatgg cggcgtttat
    25381 ttgcttcacg gtacgaacgc cgatttcggc attggcatgc gggtaagttc tggctgtatt
    25441 cgtctgcggg atgacgatat caaaacactc tttagccagg tcaccccagg caccaaagtg
    25501 aatatcatca acactccgat aaaagtctct gccgaaccaa acggtgcgcg tctggttgaa
    25561 gtacatcagc cgctgtcaga gaagattgat gacgatccgc agctgctgcc aattacgctg
    25621 aatagcgcaa tgcaatcatt taaagatgca gcacaaactg acgctgaagt gatgcaacat
    25681 gtgatggatg tccgttccgg gatgccggtg gatgtccgcc gtcatcaagt gagcccacaa
    25741 actctgtaaa tcctggataa aaaaaatccc cgcgacacgg catgtcgcgg ggaaaaaatc
    25801 agcagtagca ggcattaatg agggttaatg cttatttata aattactgct gttccatgga
    25861 gggtattcgg accggttaca gaagtaatac ggaaagattt tgcgcccatc tcatccgctt
    25921 tttgcgccag ctgctcttcc agcgatccca gatttgtccc cgcgttagca ctgatagtac
    25981 cgactttttg ttggccttct ggcgttgact gaacttcgac agccgcaaag ttggcaaatg
    26041 acatggagct taaaatcgcc gcagcgatga gggtttttac gtttttcata atagtggcct
    26101 tatgcagatg aatgacgtcg caagaaatta cttaacgatc gataactaaa tgatagatgt
    26161 gatctggatc acatacaaga tattttttat aacaatcatt aattaattta aaaatcttta
    26221 aatttcagtt aaatagaaag caattatttt tatgggatct ggactggtga acggaggtga
    26281 ttatttttat aataaccttt cattcacagt caccaggttc aacggcatca tggcaactga
    26341 ctcaacacaa tgtgtaaaaa aaagccgtgg ccgcccaaaa gtgttcgaca gggatgccgc
    26401 gcttgataag gccatgaaat tgttctggca acacggttat gaagcgactt ctcttgcgga
    26461 cctcgtcgaa gcgaccggag ccaaagcgcc cacgctatac gcggaattta ccaacaaaga
    26521 ggggttattt cgtgccgttc tcgaccgcta tatcgatcgt tttgccgcta agcatgaagc
    26581 acagctgttt tgtgaagaga aaagcgtgga gtctgcgctg gctgactatt ttgctgccat
    26641 cgccaactgc tttaccagca aagacacccc ggcaggctgc ttcatgatca acaactgcac
    26701 caccctctcc ccagattcag gagatatcgc caatacgttg aaatcacgcc atgcgatgca
    26761 agagcgcact ttgcagcagt ttttatgtca acgacaagcg cgcggggaaa tcccgaccca
    26821 ctgtgacgtg acacatctgg cagaattcct taattgtatt attcagggga tgtcgatcag
    26881 cgcacgcgaa ggtgcatcgc tggaaaaact gatgcagatt gccggaacga ctttgcgttt
    26941 atggcccgaa ctggtgaaat aagtcgcaaa aaaagcccct cagcctgagc gaatgagggg
    27001 ccaaattgca gagtacagcc ttgttatact ttgttattca aaatcagttg cccgttacta
    27061 tctagcggga tctgcgtacc cggatcgcgg tccatgcgga ttttgccctg ctgatcgcca
    27121 atcttatagg tcacatcata accgagcatt ttttctgact tgtcatacac cgttttacaa
    27181 cgctgttgcg tagtcgtgta agtatcgctt tcctggagag agccctggat ctggttaccg
    27241 gcatatccac cacccagcgc ccccacaaca gtggcgacat ctttaccacg accaccacca
    27301 aactgatgcc ctatcacgcc gccagcaaca gcgccgagca ccgacccggt aatgcgattt
    27361 tcatcctgca ccggtcgacg atgggtcact gtgacgttgc gacactcctg acgcggtgtt
    27421 ttaaccgttt ccttgattgg ggttgcagaa acaacctgag cgtattgcgg gccccgttca
    27481 aacacgttca gactggccac tgccgctacg cccagcgcag ctgcgacacc aatcccgata
    27541 cccgccaaca ttgatttatt cacgggaaca cctccttctt tgcagttatg ccaatcatgc
    27601 aagcgaagga gaaaagcagc tatcagacag aagatgaaaa gcgcgggaat ggctggaaat
    27661 acttaaggat tcggagaagt ctgaaggaat gcctgatgcg acgcttaacg cgtcttatca
    27721 ggcctaccgc tcgtaccatt tgtccagggc ggatgaggcg tttatgccac atccgccagt
    27781 gtacgtcgat taatgcaact tcaaacgcgg acggataacg cggttaatac tccccaccag
    27841 catcattaat ccggttttaa agtaaccatg cagcgcaatc tgatgcattc ggtatagcga
    27901 gatatataca aagcgcgcaa ttcgtccttc aatcatcatt gagccgcgcg tcaggttacc
    27961 catcaggcta ccgacagtgg agaagttcga cagcgatacc agcgaaccat gatctttata
    28021 ctgataattt ttcagcggtt taccgttcat ctgcgccaga atatttttca ttgcgcaggt
    28081 cgccatctgg tgtgcagcct gagcacgcgg cggaacaaag cccccttccg gacgcgggca
    28141 tgacgcgcag tcgccaatag cgtaaatgtc tggatcgcgg gtggtttgca gcgtcggttc
    28201 caccaccagc tggttgatac ggttggtttc aagaccaccg atatctttca ggaagtctgg
    28261 cgctttgatc ccggctgccc acaccatcag atcagcctca atatattcac catctttagt
    28321 gtgcaggccg ccttcatcag cactggtgac catggtttgc gtcagcacgc gaacgccaag
    28381 tttcgttagc tcgttgtggg ccgcagcaga gatacgtggc ggtaatgcag gcaaaatacg
    28441 ttctcccgct tctaccagcg ttacgttcag ggcttcgttg gtcaggcctt tgtaaccgta
    28501 gctgtgcagt tgcttgaccg cattgtacaa ttcagcggag agttctacac ccgtcgcgcc
    28561 gccgccgaca atcgcaatgt tcactttacc attcgcgccc aggttggcgg agtatttcag
    28621 gaacagattc agcatctcct ggtggaagcg acgcgcctgg tgcgggttat cgaggaaaat
    28681 gcagttctct ttgacacctg gcgtattgaa atcgttagag gtgctaccca gcgccattac
    28741 cagggtgtca taggcgattt tacgttccgg aaccagcagt tcacctttct catcgcgcag
    28801 ttctgcaata gtgattgttt tcgcttcacg atcaatatca atgacggaac ccagctggaa
    28861 ctggaaacca tgattgcgcg catgggccag atagctcaac gcatcgacgc cttcatcaag
    28921 cgagccagtc gccacttcgt gcagcagcgg tttccacaga tggctgtggt tacgatcgac
    28981 cagcgtaatt ttggcttttt tcttgcgtcc cagcttatgc cccagctgtg ttgccatttc
    29041 cagcccacca gcaccgccgc cgacaatcac aatttttttc aatggcgtag tcaacgtgac
    29101 ccccttaaat ttattaacca attgttaatt aatggttata aacatagctc ttaattaaca
    29161 acaggttaca gcaatgaaaa tgttcttcgc tattgagaat aacatgaatg gtgcattggt
    29221 cataccaaaa ttgatgttaa tcaagttttg ttgctgaaaa gataggcgac tttaccgggt
    29281 gagatcaaaa aacagagttt attgtttatt atttgagcaa gtccggaagg gtgatatagg
    29341 gtgacgcgta tatcgagggc gtttggtcgt cctgataaga tgcgcagcat cgcatcaggc
    29401 attgatgctc gactgtcgga tgcggcgtaa acgccttatc cgacttacag aattcggagc
    29461 gcatcaggta ccggcaaggt tacctgatgc gacgagggca tttacccgag ggttttgaac
    29521 gccttaatgc gctgcaaatg cggggagata ttcttgaatt tgtgcgtctg ctcttcgtcc
    29581 cagacaatct cgtaataatg atgcaactct tcagatgtcc gctggctgtt aagcgcttca
    29641 tcattacgcg acaaaatcac caggcaacga tcgcgattct tctcgcggaa gttggtcaca
    29701 cacttagtgg caatatcggc atactcttcc gggcgatcaa tcttgccttc catgttctca
    29761 taagggaaca aattagggtt gaagatcacc tggcggatat cgcaaagaaa accaatccgt
    29821 tccgcccagt atccgcccag accaacgccg caaatcagcg gacgctcgtc gacgttcagt
    29881 tgcaacattt tgtccacttc tttgagcaga tgctgcatat catgtttcgg atgccgcgta
    29941 ctgtagctta tcaagcgtac atccgggtca ataaactgca attgtaagac tttctcgtgg
    30001 ttacccggac tgttagagtc aaaaccgtgt aaatagataa tcatcgcttc ctcacataag
    30061 ccagggttag tgacctgctt tctcttcctg ccagcgttca tgtaactgat tcagacgggt
    30121 gctgatcgcc ttccagcgag ccgagtccat cagttcctgt cgcgaaaatg aacctttatg
    30181 atacaaacgt gtaacacgtt ctgccttgat cggtgacaga ttatctaaca cgctgacggc
    30241 ccctttacga ttattgcaga ccaggatcat atcgcaacct gcgtccagtg atgcctgccc
    30301 gcgttcggca taactgccca taatcgcggc accttccatc gataaatcgt cagagaaaat
    30361 tacaccgtca aaacacagtt cctgacgcaa aacggttttc agccagtagg aagaaccgct
    30421 cgccggacgc ggatcaacat cactgtagat cacatgcgca ggcataatgg cgtcgagttt
    30481 attttcgcgg attaaggaac tgaagaccga catatcttta gcgcgaatct ccgcttgtgg
    30541 acgtggatcg cacggtgttt ctttgtgtga gtctgccgtt actgcaccgt gtcctgggaa
    30601 gtgtttcccg gtcgttttca ttccggcttc atgcatacca tcaataaacc ggctggcaat
    30661 tgccagggct ttttgtggat cggcatgata agaacgctcg ccaatcgccg cgctgatatg
    30721 cccgacatcc agcactggcg caaagctgat atcaatatcc atagcgatca tttcgctggc
    30781 catcagccaa cccgcctctt gcgccagttt gccgccctct tccattccca acagcgcagc
    30841 aaaggattgt gctgccggta agcgggtaaa accttcgcga aaacgctgca cgcgtccacc
    30901 ttcctgatct accgccacca ccagatgatt gcgcgatgct gcgcggatct ggcgcaccag
    30961 ttcacgtaac tgggcaggat catgatagtt acgcgtaaag agaatcagcc ctcccaccag
    31021 cggatgcgcc agtatttcac gctcttccgc gtccagttcg taaccttcga catccaacat
    31081 tactggaccc acactgctct ccttattgtt ttattaatag ctgccgccag gtgtcatcgg
    31141 ccagcctgat aaattgttga tcgccgattt gtcgccagcg gtactcaaac caccctgctt
    31201 tgagcatcag cagccaggga aaccatcgcc tgacctgacg ccataattgc gccggataaa
    31261 tcttcgcgcg agtggcatag tcattgacca attgccggtg ctgttcagta ttttccaccc
    31321 acaccgccgc cagttccagc gcgatatcac catctccggc atactcccag tcgatgagtt
    31381 ttaaccctga cgcgctatgc actaaatttc cggcgtggac atccatatgc aatggactta
    31441 agcgtaaagg ccgtggttcc cgcgctttgc gcagacgttt taacattcgc agccaaccca
    31501 cagtccgccg cgccggatcg ctttgctgcc agtacagttc cagtaagggc aacagcgtta
    31561 ttcgccagcc aaaacgtggt tgttgatgaa gataatacag caagcctgcc agttcgttgg
    31621 tatccggcaa atacgttttt accgcgccgg gcagatagtc gactaccatc cagtcacgga
    31681 gatataaatg cggcttcggt gcaatgcttg cgggtagttg tgataaagcc cgatactggc
    31741 gcaagaacgc ggactgcggc gcatcaggat cgtgcggctg acgcacaaca aaacgctggc
    31801 cctgatgttc aatgagaaaa ctaccgccac taagcccact attaaacgtc gtgacgggat
    31861 gaaactgcgg gaaaaagcgc gacagcaatt cgtcgcgcgt gatgggatta ttgctgcgaa
    31921 acggcacctt tacctgacca gataatttcg cccgtctgca ccagcatcag ctgcatttgt
    31981 agggtcggag cgttaacgtt gccagaggcg ctggagtaca gcacgtaatg agcgccgaca
    32041 ttgcgggcaa tgcctatggc tttactacgg gtacctaaac tgtcctgcgg cgacaaacct
    32101 aactgttgct tagccatcga cagctgctgg gcggaaacca gggtaaattt cccgttatta
    32161 gccagcgcat ttcgcagcgt ttcggtcgct tctgcggcat tcagcgaacc gttagtacgg
    32221 ttgttaacgc tatcaaccag caggacgcta cccgcagtca ccccgtcagc cccaagcatc
    32281 ttactgacca tcggctgcat tgcgccattc cagtcataat ggcgaatatg cggcgcaggc
    32341 ggtgccgttt gatcttcgtg ctcaattggg cctggctgct gcgggatcgt cggcaccgag
    32401 ggcactgtgg ggacaggctg ttgtggctcg gctggttgtt ccggcgctgg tttcacttct
    32461 tctaccggtg caggttcacg ttgccccaca cacccggcga gaaacatcgc cagcgcggta
    32521 atcaaggcgt agcgactcat ttttgtcatc aagattcacc ccttacaaat atagataaag
    32581 tctgactttg tgcgccccca gaaaattggc gctgccgtac agcgttaccg ccgaatgtgc
    32641 gggaatgcta acgctgcgtg gcctttccag aggatgcatc tccagccctc tggcgtcata
    32701 ccagtaaaaa cgataatgaa cggtaacggg ttcttgcctt tcgttataaa gcgttgagga
    32761 tgctgaaggt tgaatatcag acgtcgaaag gacgggcttt tctgcactga tgcccgcagc
    32821 caataaagat gactccatca ccagcgattg ctcatcattc accggaattt ccggatgtga
    32881 acgacagccc accagcagta acaacgccag agacaccagc ccaaagcatc cttttctcat
    32941 cgttacagac ctttatgcgc cagcattggt cccagcggac ggccacccaa caagtgcata
    33001 tggatgtggt aaacctcttg tccgccatgg cggttggtat tcatgatcag acgatagcca
    33061 tcttcggcaa taccttcttg ctcagcaatt tttgccgcta cggtgatcat gcgtcccagc
    33121 gcctgctcat gctcagctga cacgtcgttc acagtcggaa tgaggatatt cggaatgatc
    33181 agaatatgcg ttggcgcttg cggcgaaata tcgcgaaacg ccgttaccag atcatcctgg
    33241 tagacgatat cggaggggat ctcacgacga ataattttgc tgaatatagt ttcttctgcc
    33301 acgacatttt cctttttcaa atagccatgc gttgcgccat gctacgccgc gccacactgc
    33361 gagtatagag tatggtcgag ttaaacctgc tctttcaacc ttaactcacg atttcttaag
    33421 caaaaaatcg ttttgctgcc gtatatatcg ccattattcc catttctgcc tgcgaatgcg
    33481 ctgaaaagac attctctgtt tggtttacac gacaaatatg aatgcgcata tttctcattt
    33541 gcatttacaa acaaaattat tcgcacacta aatataaccg ctcatcaccc tcctccggca
    33601 tgagcctgac gacaacataa accaagagat ttcaaatgct ttcaacacaa tttaacaggg
    33661 ataatcaata tcaagccatc accaaaccgt cactacttgc cggttgcata gcactggcac
    33721 tattaccttc tgccgctttt gctgcaccag ccactgaaga aacggtgatt gttgagggtt
    33781 cagccacagc tccagatgat ggcgaaaatg attacagcgt aacgtctacc tctgcgggta
    33841 ccaaaatgca gatgactcaa cttgatattc ctcagtcggt cactattgtt agccagcagc
    33901 ggatggaaga tcagcagtta caaacgctgg gcgaagtgat ggaaaacacg ctggggatca
    33961 gcaaaagtca ggcggattcc gatcgtgctc tttattattc ccgtggattc cagatcgata
    34021 actatatggt tgatggtatc cccacctatt ttgaatcgcg ctggaatctg ggcgacgcac
    34081 tttctgatat ggcactcttt gaacgcgtag aagtagtgcg tggcgcgaca ggactcatga
    34141 ccgggacggg taatccatct gcggcaatta atatggttcg aaaacacgcg accagtcgtg
    34201 aatttaaagg cgatgtctcg gcggaatacg gtagctggaa caaagaacgg tatgtggcgg
    34261 atttacaaag cccactcacc gaagacggta aaatccgcgc gcgaattgtc ggcggctacc
    34321 agaataacga ctcatggctg gaccgctaca acagtgaaaa gaccttcttc tcgggcattg
    34381 tcgatgctga tttaggcgat cttacgacgc tttcagccgg ttacgaatat cagcgcattg
    34441 atgttaatag cccaacctgg ggcggtttac cgcgctggaa tactgatggc agcagcaaca
    34501 gttacgatcg tgcacgcagt accgcacctg actgggcgta caacgataaa gagatcaaca
    34561 aggtctttat gaccctgaag cagcggtttg ctgatacctg gcaagcgaca ctgaatgcca
    34621 cccactctga agtcgaattt gacagcaaaa tgatgtatgt cgatgcctat gtaaacaaag
    34681 cggatggtat gctggttggg ccatacagta attatggacc tggctttgat tatgtcggcg
    34741 gcaccggttg gaacagtggc aaacgtaaag ttgatgcgct ggatttgttc gctgacggta
    34801 gttatgaatt gtttggtcgt cagcacaatc taatgtttgg tggcagttac agcaaacaaa
    34861 acaatcgtta cttcagttca tgggccaaca tcttcccgga tgaaattggc agtttctaca
    34921 actttaatgg caatttccca caaaccgact ggtcaccaca gagcctggca caggacgata
    34981 ccacacatat gaaatcgtta tatgctgcta ctcgagtcac ccttgccgat ccgctgcatc
    35041 tgatcctcgg cgcacgttat accaactggc gggttgatac gctgacttac agcatggaga
    35101 aaaaccacac cacgccttac gctggtctgg tgtttgacat caatgacaac tggtcgacct
    35161 acgccagcta tacctctatt ttccagccgc aaaatgatcg tgacagttca ggcaaatatc
    35221 tggctccaat caccggtaac aactacgagc tgggtctgaa atcggactgg atgaatagcc
    35281 gtctgaccac cacgttagcc atcttccgta ttgagcagga taatgtcgct cagtccaccg
    35341 gtacacctat ccccggcagc aacggcgaaa ccgcctataa agcggtggat gggacagtca
    35401 gtaaaggggt ggaatttgaa ctcaacggcg caattaccga caactggcag ctgacatttg
    35461 gcgcaacgcg ctatattgca gaggataacg aaggaaacgc cgttaatcct aatctgccac
    35521 gcaccacggt taaaatgttc accagctatc ggttgcctgt catgccagag ttgacagtcg
    35581 gcggtggtgt taactggcaa aatcgcgtgt ataccgacac cgtgacaccg tatggcacct
    35641 tccgcgccga gcaaggtagc tacgcgctgg tggatctctt cacccgctac caggtgacga
    35701 aaaacttctc gttacagggg aacgtcaata acctgttcga caaaacctac gataccaaca
    35761 tggaaggttc tatcgtctac ggcgcaccgc gtaatttcag cattaccggc acgtatcaat
    35821 tctgaataac acctgtaaaa aaggcagcca tctggcggcc ttagtctccc caacgtctta
    35881 cggattagtg gttacggatg tactcatcca tctcggtttt caggttatcg gatttagtac
    35941 cgaaaatcgc ctgaacacca gaaccagcaa ccactacgcc cgctgcgccc agtttcttca
    36001 ggccagcctg atccacttta gacacatcag caacgctgac gcgcagacgg gtaatacatg
    36061 cgtcgaggtt agtaatgttt tctttaccac caaatgcagc aaccagagcc ggtgccattt
    36121 cgctggtacc tgttgctttt gcatcttcag tcgcgtcttc acgacccggc gttttcagat
    36181 ccagtgcttt aatcagcacg cggaagatgg tgtagtaaac aatcgcataa ccgataccga
    36241 cgatcgggaa cagccacagt ttgctgctgt taccagacag aacgataaag tcgatcagac
    36301 cgtgcgagaa cgacgtaccg tcacgcatcc ccagaagaat acagattggg aatgccaggc
    36361 ctgccagaat cgcgtggatg atgtacagga tcggcgcaac gaacatgaag gagaactcga
    36421 tcggctcggt gataccggtc aggaacgagg tcagcgccgc ggagatcata ataccgccca
    36481 ctttcgcgcg gttttctggt ttagcagagt gccagatagc aattgcggca gctggcagac
    36541 cgtacatttt gaacaggaag ccaccagaca gtttacccgc agtcgggtca cccgccatat
    36601 aacgcggaat gtcgccgtgg aaaacctgac ctgctgcgtt ggtgtattca ccaatctgca
    36661 tctggaaagg tacgttccag atgtggtgca gaccaaacgg caccaggcaa cgttcgatga
    36721 aaccgtaaat gccaaacgca actaccgggt tctggtaagc agcccactga gagaaggtct
    36781 ggattgcaga accaatcggc ggccaaatga aggacagcac aacgccagta aagatggcag
    36841 ccaggccaga aatgatcggc acaaagcgtt taccggcaaa gaagccaaga tactcaggca
    36901 gcttaatacg gtagaaacgg ttaaacatgt acgctgcgat cgcaccggag ataatccctc
    36961 cgagtacgcc agtatccgcc aggtgtttag aggcgatttc ttcagcaggt aaatgcagta
    37021 ccagtggcgc aaccacggcc atggttttaa ccatgatgcc ataggcaaca actgcggcca
    37081 gcgcggatac gccatcgtta ttggtaaagc cgagggcgac accgatcgca aaaatcagtg
    37141 gcatgtttgc aaagacggaa ccgcctgctt ctgccataac atgcgataca acggcgggca
    37201 gccagctgaa attcgcggaa ccgacgccca gcagaatacc tgcgataggc agtacggata
    37261 ccggcagcat cagcgattta ccgacctttt gcaggttagc aaatgcattc ttaaacataa
    37321 ttgagagtgc tcctgagtat gggtgctttt tttacgttct cacgcgtggc aaggggggag
    37381 agcctcgccg tgtacagggc atctaagcgc cctttattta ttacacagag taaaataatt
    37441 cagtgccgat ttgtttgacg gctatcacat ttcaacttca gatcattgga gaagttgcac
    37501 attttgacaa aacatatgtc acaatatttc aaacgtggtt attcaccgcc caattttcgg
    37561 cggtgaactt tactcgtttt agccattaat tacgagcttt aaaaaaactc attcaacgga
    37621 tggattgaag gcgggaagcg tcgatgtgaa acagacgggc gaagttatcg gtggttacct
    37681 gcgccagttc ttcaacggca acacctttca acacagccat gtattctgca acgtcacgaa
    37741 ccatcgcagg ttgattctct tttcctcgat gcggtaccgg cgcaaggtaa ggtgagtcag
    37801 tttccaccag taaccgatcc agggggacat aacgcgcagc atctcgaagt tgctccgcat
    37861 tacggaaggt cacaatgccg gaaaaggaga tgtaaaatcc gagatccagt aatttacccg
    37921 ccgtttctct gtcctctgta aaacagtgta gtacgccacc gcaatccgtc actttttctt
    37981 cgcgcagaat tgccagcgta tcggcgcggg cgtcacgggt atggacgata accggcttgt
    38041 tcagttcacg accaatctgg atatgatgga tgaaggactc ttgctgacga actttagttt
    38101 ccggtgtgta ataataatcc agcccggttt cacccagcgc tacaacaccc tcttctgccg
    38161 ccagacggcg taaatcttct acatcgtagg gatcattctg gttgagtgga tgcacgccac
    38221 aagaaaatac gacgttgtca cgttcgccta ccagatcccg catatgtaaa taacccggta
    38281 atgttgtggc gactgccaga caaaatttca catcgcgtgc ggcggctttc gccagaacgt
    38341 catccacgtc cttatgcaaa gattcataat ccagaccatc gagatggcag tgtgagtcga
    38401 ctaaaaacat gatgtctctc ttaaagatga ggaaccggta gcacaacgcc cggttgcagg
    38461 taatgctcaa tacgcagtaa aagatcggtg atgagaagct cgcggttgat gcctgtgaca
    38521 gacattaact gttcacgaat gtggcaaaca tcccccagta tagcctgcag gcgcgaggga
    38581 gaaagatggt ttgccagttc gacgacaagg cccggcacat caacattggt cacctgcgca
    38641 gcaccatgat ggcgttttag cgcatccatc agcaacgttg ccagccagtg taaacgcgcc
    38701 ggagcttgtt catgattaag ggccgccagc agcgaatacc agtcgcccga tggcacgcta
    38761 tatgccaatg cctgacacaa tgtttcacga gcctgccagt tatctccctg aaacaacgcc
    38821 agtgccgcgc caggcgaacc ggcgcttaag cgcaatgcgg caagtaatgc atcctgtgac
    38881 attgtcactt cgcgtgaaag ccaggtcacg gcgtactgtt ccggcggcgg cgcaaggtaa
    38941 tgtaaccgac aacgactacg taatgttgcc agtaaacgtt caggctcgcg ggtagccagg
    39001 aaaaaccagg tttctgctgg tggctcttca agcgttttca gcaatgcgtt agccgcggcg
    39061 tcggtcagta aggcagcatc ggttacccag acaactttcg caccacctag gcgtgcgtgc
    39121 tcattcagct tttcggtgac ctcacgtact gcatcaatgc ccagcgtatt tttccctttt
    39181 tcgggagcca gggtgtagta atcgggatgc gtgccagcct gcatcaactg acatccacga
    39241 cagtgaccgc aacttttgtg gccctgcggt tgttggcaga gtaaataacg gctcagggcg
    39301 tagattaaag catcatcgcc catgcccggt aacgcctgaa tgagtagcgc atggtgacct
    39361 cttccggcct gatagctggc taccagtttt tcgaaatcag gtcgtaacca tggataccat
    39421 ctcatgcgtc caactccttc acccagtggg tcacggtagt gcggattgca tccatcacgg
    39481 cctccagcgg ctgggtggca tcaatggtat gaatactttt atcttgtgca gccagttcca
    39541 gatagcgggc gcgggtgcga ttaaagaaat cgaaagactc ttgctcaata cgatccagct
    39601 cgccgcgcgc acgcgcgcgt tttaagccaa cttccggggt aacatcgaga tagagcgtta
    39661 agtcggggcg aaaatccccg agaacagcat cacgcagtgt tgccagcata tgttggtcaa
    39721 taccacgtcc tccgccctga tacgcctgag tggagagatc gtggcgatcg ccaatcaccc
    39781 aggtgccgtt agccagagct ggtttgatga ccgtttctac cagttgaacg cgcgcggcat
    39841 aaaacatcag aacttcggct ttatcggtaa tgacttcatc gcctaccgat ttgatatcca
    39901 gcaccaggct tcttaacttt tcggcaagtt gcgtaccgcc aggttcccga gtgaaaacca
    39961 tgtcgcggat acccagttgc tcaagcgtct caaccaccac attacgcgcg gtagttttgc
    40021 ctgcgccttc cagcccctca atgacgatat acttactgcg catttttttc cttaagcact
    40081 ttcagataat cctgcacaga cttgttatgg ctggcaagat tggtattaaa cgtgtgacca
    40141 cctttaccat cggccacaaa atagagatac ggcgtttttg ccggatgcgc agcagccttc
    40201 agcgaatccg cccccggcgt agctatcgca cccggcggca aaccggtaat ggtataggtg
    40261 ttatacgctg tcggcgtttc caggtctgca cgagaaagtt tgccattata acgctctccc
    40321 atcccgtaaa tcacggttgg gtcggtctgc aggcgcatac caatgcgtaa acggttgata
    40381 aataccgagg caacctgatc gcgttcactg gcaacggcgg tttctttttc gataattgat
    40441 gccatcgtca ccagctgatt tttatcttta taaggcagac cgtccgcacg cccttcccag
    40501 gcgctatcga ccgctttcac cattttcttg tgcgctcgct tgagtaacgc gacatcggtg
    40561 gtattggcgg tatacatcca ggtgtctggc cagaaccaac cttcaatcca ctccgggttt
    40621 tccagttcaa gtgcctgcgc tacggtggcg tacttatcgt cactcagcgt gtgcttgata
    40681 tacggagcct cacgcaattg cttgaggtag tcgctcagac gcatcccttc taccagtcgc
    40741 agcgggaact gtgcttcttt accgctttcc agcaatttca gcatctcgcg cacggtcatc
    40801 tgcggtgtaa agcggtaagt cccggcttta aagtgagaaa gatccggttc gatacgcagc
    40861 aaccattgaa aaacccgtgg gcgattgatg atcttatcgg cataaagctg ttcaccaagc
    40921 gccagacgtc cggtccctgg cttcagggta aatatcgtct cttctttgat aagcaatttg
    40981 ctgtcggcaa gatggcgaac cttccagacg cccacaccag cggcgatacc cagtaccacc
    41041 agcaataaca agataatcaa taacactttt ttcatgacta attcgggcgc tcacaaagtg
    41101 gggctaaata ttcatataac gttgctgacg aaaaggagac atcgccacag gcacgtacgg
    41161 gcatcactgg cattaacgca ttacaaataa ccatctcatc tgcctgcaac gcctcttcca
    41221 gagaggcttg cacttcgaca agctgataag aggattgtgc cagcaaacgg atacagaatt
    41281 gtcgcataat gccgttaaca cctgcctgat ccagacgcgg cgtataaact acattgccct
    41341 tccgccagaa caaattagcc gcacagcatt ccgtaaccca cccttcgctg tcaaggacca
    41401 gcgcctcatc agcgtttgtc tgctcaagat gagagcggat caatacttgc tcaagccgat
    41461 taagatgttt aatacctgca agatgaggat tgcgccccag ccgcaccggg cttagcgcca
    41521 acgtcatccc ttcgttacgc aaacggtcgt aatgtgcagg ataaaccgta acggagagaa
    41581 tccgcgttgc cggtccactg ttcaatgtgc tgtaccctcg cccgccacta ccgcgactga
    41641 tcacgacttt cagcacacca ttttgctgtt ctgctgccag cgttttcatc tcttgttcaa
    41701 gctgaggcca gaagtcacag aaaatcatca accgctgaca agcatcctgt agtcgctgga
    41761 tatgcgccga taacaaactg actttaccgt cgataactct ggcggtggta aaacaaccat
    41821 caccaaactg cgttgcccga tcgcttactg ccagcgattc ctgcttatga ccgttaatta
    41881 agaacatacc ggctccttat cagagaattg ttagtgtggc agcatgttca ctacggaaca
    41941 agtcggaata aaagctaaga aaaaaggccc gcaagcggac cttttatatg ggtgggaaat
    42001 gacaacttag atctttttaa agatcaaaga accattagtg ccaccgaagc cgaaggagtt
    42061 acacagcgtg tattccattc cgctgacctg acgtgcttca tgcggtacga aatccagatc
    42121 gcaaccttca tccgggttat ccaggttgat ggttggcgga acagcctgat cgcgcagcgc
    42181 caggatggag tagatagatt ctactgcacc cgccgcacct aacaggtgac cggtcataga
    42241 tttcgtggag cttaccaaca cacggcttgc agcttcaccg aagatggttt tcaccgcctg
    42301 cgcttcagct ttatcgccag ccggcgtaga agtaccgtgc gcgttaacgt agccaatctg
    42361 actcgcttca atgcctgcat cacgcagagc atttgccatc gccagagctg cgcctgcgcc
    42421 attttctggc ggtgacgtca tatgataagc atcgctgctc ataccaaagc cgacgagttc
    42481 agcgtaaatt ttcgcaccgc gttttttcgc gtgttcgtac tcttcaagta ccagcatacc
    42541 ggcaccatcg cccagtacga agccatcacg ctctttatcc cacgggcggc tcgccgcttg
    42601 cgggttatca ttgcgggtag ataatgcacg tgccgcgcca aaaccaccaa cacccagcgg
    42661 cgtactggct ttctctgcgc cacctgcaac catcacgtca gcatcgccat acgcgataat
    42721 acgcgcagca tggccaatgt tgtgcacgcc ggaagtacag gcggtcgcga tagagatgct
    42781 tgggccacgc aggccataca tgatagtcag atgacctgcc accatgttca caatcgttga
    42841 cggaacgaag aatgggctga tcttacgtgg accaccgttc atcagagatg tgtggttttc
    42901 ttcgatcagt ccgaggccgc caatcccgga gccaattgcg gcaccaatgc gggttgcgtt
    42961 ctcttccgtt atatcaaggc cagaatcctg catggcctga acgccagcga caattccata
    43021 ttgaatgaag gcatccatct tgcgctgttc tttgcgcgag ataatgtcct cacagttaaa
    43081 atcctttact aagccagcaa atttcgttgc ataggcgcta gtatcgaaat ggtcgattag
    43141 gctgatgcca ctctgaccgg caagcagagc tttccaggta gactctacgg tattgccgac
    43201 aggagacaac atgcccagtc cggtcacaac tacacgacgc ttagacacgt ttgtcctcca
    43261 gggagggaaa aaatgattct agtgggacaa aaagataaaa ctcaggcggt cgaacgaccg
    43321 cctggagatg ttcacttacg cctggtggcc gttgatgtaa tcaatggcag cctgaacggt
    43381 ggtgattttc tcagcttctt cgtccggaat ctcagtatca aactcttctt ccagagccat
    43441 taccagctca acggtgtcaa gagaatccgc gcccaggtct tcaacgaaag aagcattgtt
    43501 ggtaacttct tcctgcttaa cgcccagctg ttcgccgata attttcttaa cgcgttcttc
    43561 gatagtgctc atactcttaa atttcctatc aaaactcgct ttcgcgatgg ttttcgtagt
    43621 gtataaaatg ttgaaaaatt tgcaactaaa tcccggcagg tcttaccacg attttacgtt
    43681 attttgaggc cttacccccc aataacgcaa atcattttgc actaattatt actcataacc
    43741 acgcagactg cgcgcaacat gagcattttg tgcaaatcgc ggtcagacca tgtacatccc
    43801 gccgttcaca tgcaaagttt cacccgtgat gtaagctgct tcgtcggatg ccaggaatgc
    43861 aaccgcgttg gcgatttcct gtgcgccgcc gaggcgaccc gcaggaacct gcgccaggat
    43921 acccgcacgc tggtcatcgc tcagcgcacg tgtcatgtcc gtttcaataa agcccggagc
    43981 aacaacgttt acagtaatac cgcgtgacgc aacttcgcgc gccagtgatt tactgaagcc
    44041 gatcaagccc gctttcgccg cagcgtagtt ggcctgaccg ccatttccca tggtaccaac
    44101 cacagaaccg atagtgataa tacgaccatg acgctttttc atcatagcgc gcattaccgc
    44161 ttttgacaga cggaaaacag atgaaaggtt ggtttcgata atatcgttcc actcttcatc
    44221 tttcattcgc attaacaggt tatcacgagt gataccggca ttattgacca ggatatccac
    44281 ttcaccaaat tctgcgcgaa ttttttccag aacagattcg atagatgccg ggtcggtcac
    44341 attcaacatc agacctttgc cgttggcacc taaataatcg ctgatcgcct gagcgccatt
    44401 ttcactggtc gcagtgccaa taactttcgc gccacgggct gcgagcgttt cagcaattgc
    44461 gcggccaatt ccgcggcttg caccggttac cagtgcgatt tttccttcaa aattcatgat
    44521 tttcctcttt taaagctcaa gcgccgctgc catcgctgaa ggttcgttca gcgccgaggc
    44581 ggtcagggtg tcgacaatgc gtttcgtcag gccagtaagc accttgcccg ggccgacttc
    44641 atagagatgt tctacgcctt gcgctgccat gtactcaaca gacttcgtcc actgaaccgg
    44701 gttatacaac tgacgtacca gtgcgtcacg gatggcatca ccattggttt cgcatttcac
    44761 atcaacgtta ttcacaacag aaactgttgg tgcgttaaag gtgattttcg ctaattctac
    44821 tgccagtttg tcggctgctg gtttcatcag cgcacagtga gacggtacgc tcactggtaa
    44881 cggcagagca cgttttgcgc ccgcagcttt acaggcagcg ccagcacgct caaccgcttc
    44941 tttatgaccg gcaataacca cctgtcccgg agagttaaag tttaccggag aaacgacctg
    45001 acctgctgca gcttcttcac acgctttcgc aatagacgca tcatccagac cgatgattgc
    45061 cgccatagcg cccgtacctt ccggtacggc ttcttgcatg agcttgccgc gcatctcaac
    45121 cagacgcacc gcatcagcga aatcaatcac accagcgcaa accagcgcgg aatattcccc
    45181 caggctgtga ccggccatca ttgccggtgc tttaccgccc tgctgctgcc atacgcggta
    45241 cagcgcaaca gaggcagtca acagcgctgg ctgggtttgc caggttttat tcagttcttc
    45301 agctggcccc tgctgggtca gcgcccacag gtcgtagccc agcgccgcag aagcttcagc
    45361 aaacgtttct tcgacaattg gatagctcgc cgccatatca gccagcattc caacggtttg
    45421 agaaccctgt ccagggaaca caaatgcaaa ttgcgtcatg ttttaatcct tatcctagaa
    45481 acgaaccagc gcggagcccc aggtgaatcc accgccaaag gcttcaagca gaaccaactg
    45541 ccccggctta atgcgcccgt cgcgtacagc ttcatccagc gcgcacggga cagaggccgc
    45601 agaggtatta ccgtggcgat ccagcgtcac cacgacattg tccatagaca taccgagttt
    45661 tttcgccgtt gcactgataa tacgcaggtt agcctgatgc ggaaccagcc agtccagttg
    45721 agaacggtca agattatttg ccgccagcgt ctcatcaacg atgtgcgcca gttccgttac
    45781 cgcaaccttg aagacttcgt tgcccgccat cgtcagatga attgaattct ctggattcac
    45841 acggtcagca ttaggcagcg tcagcaactc accatagcta ccgtcggcat gcagatgggt
    45901 ggagatgatt cccggctctt cagaggcagc cagcaccgca gcgcccgcgc catcgccaaa
    45961 aataataata gtcccacgat cggttggatc gcaggtgcgc gccagtacat cggaaccgac
    46021 gaccagagca tacttcaccg ccccagattt cacgtattga tcggctacgc ttaatgcata
    46081 ggtgaaacct gcgcaggctg ctgcaacgtc aaatgccggg caacctttaa tgcccagcat
    46141 gctttgaatc tgacaagctg cgctcgggaa agcgtgcgta gcagaagtcg ttgccacaac
    46201 gatcaggcca atctggtctt tctcaatgcc cgccatctca attgcgcgtg tcgccgcttc
    46261 aaagcccatg gttgaaacgg tttcgtttgg cgcggcaatg tggcgttcgc ggataccggt
    46321 acgagtgaca atccactcgt cagaggtgtc caccattttt tccaaatcgg cgtttgtccg
    46381 cacttgttcg ggcagatagc tgccagtacc aataatcttc gtatacatgt acgctcagtc
    46441 acttttcggt tatataccgt cacttgcaaa ctgcgagttc gctggcagcg tcctgctacc
    46501 gcagagttcc gcttttgcca ccgtccagca gctcaaaacc agctgggtat acagattcca
    46561 ggcgagcggc aattcgctga ggaacttgtc gctgcaccgc ctgcactgcc tgttcaatcg
    46621 cgaccgcaaa agctcgctga ttggctgcac catgactttt tatcaccgtg ccgcgcaatc
    46681 ctaacagaca ggcgccgtta tactggtcgg ggttgaggtg actgaatcgc ctcgtcaggc
    46741 tcttttgtag ccaacgcttt aataacagta gccaccacga ccgttttttc ccttcaccct
    46801 gagatttcag cagagaaagg aacatcctga caacaccttc catcgtcttt aatgtgacat
    46861 ttcctgtaaa gccgtcacaa accagcacat ctgtcttgcc agttaacaac tcattggctt
    46921 caagatagcc gatataattg atagaaggga ttgttttaag caccgctgag gcatcccgaa
    46981 tactgtcgag accctttact tcttcttcac caatattgag caacgccacg cgaggattgg
    47041 gaatttccac cacctcttca gccagaactg agcccataat ggcaaattgc accaacattg
    47101 tactgtcaca atcgacgttg gcccctaagt caaggaccac cgttttgccc ttttgctgat
    47161 gtggtaatac cgtcaccagc gccggacgct caatcccctc caggggcttg agtaataatt
    47221 ttgccagccc catcagtgcc ccggtattac cggcactgac acaggcttgc gctcgacctt
    47281 ctttcaccag ctccagggcc acgcgcattg aactcccacg actggcgcgg atagcttgcg
    47341 aaggccgggc atcactggcg ataactgact gcgcaggaat aatctgcaga cgcgaacgtt
    47401 gttcaaagtc agctttagca agtaatggcg tgatggcgtc gggattgccg actaaaagaa
    47461 gagtgagttg cgaattagaa ttcagtgcct gcaatgctgc aggcactgtc acggaagggc
    47521 caaaatcccc tcccatgaca tctaacgcca gggttagacg tgtcaaggta tcgtcgccgc
    47581 ccggtttggt ctttccccag ttgcctgggg aaaatcctca ctaagcttca tcacgcagac
    47641 gcgtgattac ttagcgatga ccttgcggcc gcggtagtaa ccgtcggcag tgatgtggtg
    47701 acgcaggtgt ttttcaccag aagttttgtc tacagacagg ctggtgactg cggtcagcgc
    47761 gtcatgggaa cgacgcatgc cacgtttgga acgggttggt ttattctgtt gtacggccat
    47821 ggaccttact cctcaattac ggtttatccc cgatccaacg gggagcacca attacttacg
    47881 ctttaagctg gctaatacgg caaatgggtt tggcttttgc acttcttcag gcagttcacc
    47941 aaagaccatg tccgcttcgg acacttcaca gtgttcagaa tcatgcaccg gaactaccgg
    48001 caaggcgagg atgatttcat cttcaaccat tgcaagcaga tcgatttcac cgaattcgtt
    48061 aacctcaatc ggttcatacg cttccggcag tgcttcagcc tgttcgtctg aacgcacagg
    48121 actaaaacaa tacgttgtgt agacctgatg agtaaacggc ttcccgcaac gctgacactc
    48181 gagcgttacc gtcaccttcg catcgccgtt taacactgcg agacgttggt tatcgatagc
    48241 gaacgacatg gagcattcca catcactgtc cacactgact acggattcgg cgacgcgctc
    48301 aacctgatca ggggtataga taccctggta atcaaggcgt ttttgagccg tacgaaccgg
    48361 atcgagagtc aggggtaatt ttaccttttg catagggcgc gcatattaac tttgtaacgt
    48421 catagagtca aagaaaaagg cagcctgtgg ttgccttttg ccaataattc gcacacattg
    48481 cgggttactg ctttattttc gttcagtgct gataacgaac atcaacctgc aaaacgaagc
    48541 gcatagttta aaatggctat catcaatacg ctatatctga tggaaaaaaa tatgcctaaa
    48601 cttattttag cctccacctc gccatggcgt cgcgctcttc tggaaaaact gcaaatctct
    48661 tttgaatgtg cagcaccaga ggtcgacgaa accccacgca gcgacgaatc accgcgacag
    48721 ttggtgcttc gactggcaca agaaaaagcg caatctctgg cgtcacgtta tccggatcat
    48781 ttaattattg gttcagacca ggtatgtgtt cttgatggcg aaatcaccgg taaaccgtta
    48841 acggaagaaa atgcccgtct gcaattacgc aaagccagcg gcaatatcgt caccttctat
    48901 actggactgg cgttgtttaa ttcggcgaat gggcatctac aaacagaagt ggagcctttt
    48961 gacgtccatt tccgtcacct gagcgaggcg gagattgata attacgtgcg taaggagcat
    49021 cccctgcact gcgcgggtag ctttaagagt gaaggatttg gcattacgct gtttgagcgt
    49081 ttagagggac gtgaccctaa cacgctggtt ggtttaccgc taatcgcgct gtgtcagatg
    49141 ttacgtcggg aagggaaaaa cccgctgatg ggatagttgt ttgcgctgga tgcggttacg
    49201 tcgcatccgg caatcggtgc aggcggcctg atgcggcgta aacgccttat caggccaacc
    49261 gcccattatc aggcttatat gcttgtatta gcgcgcgtta cgcagctttt gcaaacaacg
    49321 cttcaaacct tcatccatcg gcgcttcgat acgcatcacc tcaccggtcc ccggatgggt
    49381 aaacttcaac gctgcggcgt gcaggaacag acgatttaat cccgtgcctg cttcagtgag
    49441 ctgtctgtca aattcacggt caccgtagcg atcgtcaaag gcaatcggat gacccgcata
    49501 ctgtgtatgc acacggatct gatgagtacg tccggttacc gggctacaac gcaccagggt
    49561 ggcaaatgca tagcgttctt ccactttaaa gcgtgtttcc gacggtttgc cttcctgact
    49621 gacacgcacg atacgttcgc cgctttgcag aatatttttc aataacggcg cttgaacgct
    49681 cttcacatgc gactgccact gaccgcgcac cagcgccagg taatcttttt gcattccttt
    49741 ttcacgtaat tgctcatgca gagaacgcaa cgccgagcgt tttttcgcta tcagcaacac
    49801 acctgaagtg tcccggtcga gacgatgaac cagttcaagg aaccgcgctt ccgggcgcaa
    49861 cgcccgcaaa ccttcgataa cgccgaagct taaaccactg ccgccatgta ccgccgtacc
    49921 ggaaggttta ttcagcacca ggatatgatc atcttcatat aagatgacgt ccgccagcgc
    49981 cgccaccttt tgcagatgtg gcgaaaccgc ctcttcttcc cgctcagcaa cgcgaaccgg
    50041 tgggatacgc acctcatcac ccgcttcgag tttatattca ggcttaatac gttttttgtt
    50101 cacccgcact tcgcctttac gcaaaatacg gtaaatcata ctttttggta cgcctttcaa
    50161 ttgggtacgc aaaaagttat cgatacgttg ccccgcttcg tcagcggtga tagcaacaat
    50221 ttttacggat ggagtctctg ttttcatggt gggcgattct aattagccaa caggattcgc
    50281 gccactcatt tttctatgct tatatttagt tttgcacctt attacttcac tacgtgatca
    50341 ctttattgat ggttattaaa ccaatcacca gcaagaagtg aaaaaactgt gagtaagcgg
    50401 gtgataaatg gtaaaagtca tcttgctata acaaggcttg cagtggaata atgaggccgt
    50461 ttccgtgtcc atccttgtta aaacaagaaa ttttacggaa taacccattt tgcccgaccg
    50521 atcatccacg cagcaatggc gtaagacgta ttgatctttc aggcagttag cgggctgcgg
    50581 gttgcagtcc ttaccggtag atggaaatat ttctggagag taatacccag tctgtttctt
    50641 tgataattgc gctgtttttc cgcatgaaaa acgggcaacc gacactctgc gcctctttga
    50701 gctgacgata accgtgaggt tggcgacgcg actagacacg aggccatcgg ttcacacccg
    50761 gaaaggcgtt actttgcccg cagcttagtc gtcaatgtaa gaataatgag taagttacga
    50821 tgaaaagaat gttaatcaac gcaactcagc aggaagagtt gcgcgttgcc cttgtagatg
    50881 ggcagcgtct ttatgacctg gatatcgaaa gtccagggca cgagcagaaa aaggcaaaca
    50941 tctacaaagg taaaatcacc cgcattgaac cgagtctgga agctgctttt gttgattacg
    51001 gcgctgaacg tcacggtttc ctcccactaa aagaaattgc ccgcgaatat ttccctgcta
    51061 actacagtgc tcatggtcgt cccaacatta aagatgtgtt gcgtgaaggt caggaagtca
    51121 ttgttcagat cgataaagaa gagcgcggca acaaaggcgc ggcattaacc acctttatca
    51181 gtctggcggg tagctatctg gttctgatgc caaataaccc gcgcgcaggt ggcatttctc
    51241 gccgtatcga aggcgatgac cgtaccgaat taaaagaagc actggcaagc cttgaactgc
    51301 cggaaggcat ggggcttatc gtgcgcaccg ctggcgtagg caaatctgct gaggcgctgc
    51361 aatgggattt aagcttccgc ctgaaacact gggaagccat caaaaaagcc gctgaaagcc
    51421 gcccggctcc gttcctgatt catcaggaga gcaacgtaat cgttcgcgca ttccgcgatt
    51481 acttacgtca ggacatcggc gaaatcctta tcgataaccc gaaagtgctc gaactggcac
    51541 gtcagcatat cgctgcatta ggtcgcccgg atttcagcag caaaatcaaa ctgtataccg
    51601 gcgagatccc gctgttcagc cactaccaga ttgagtcgca gatcgagtcc gccttccagc
    51661 gtgaagtccg tctgccgtcc ggtggttcga ttgttatcga cagcaccgaa gcgttaacgg
    51721 ccatcgacat caactccgca cgcgcgaccc gcggcggcga tatcgaagaa accgcgttta
    51781 acaccaacct cgaagctgcc gatgagattg ctcgtcagct gcgcctgcgt gacctcggcg
    51841 gcctgattgt tatcgacttc atcgacatga cgcctgtacg ccaccagcgt gcggtagaaa
    51901 accgtctgcg tgaagcggtg cgtcaggacc gtgcgcgtat tcaaatcagc catatttctc
    51961 gctttggcct gctggaaatg tcccgccagc gcctgagccc atcactgggt gaatccagcc
    52021 atcacgtctg cccgcgctgc tccggtaccg gtaccgtgcg tgacaacgaa tcgctgtcgc
    52081 tctctattct gcgtttgatc gaagaagaag cgctgaaaga gaacacccag gaagttcacg
    52141 ccattgttcc tgtgccaatc gcttcttatc tgctgaatga aaaacgttct gcggtgaatg
    52201 cgattgaaac gcgtcaggac ggtgttcgct gcgtgattgt gccaaacgat cagatggaaa
    52261 ccccgcacta ccacgtgttg cgcgtgcgca aaggggaaga aacgccgacc ttaagctaca
    52321 tgctgccgaa gctgcatgaa gaagcgatgg cgctgccgtc tgaagaagag ttcgctgaac
    52381 gtaagcgtcc tgaacaacct gcactggcaa cctttgccat gccggatgtg ccgccagcgc
    52441 caaccccagc tgaacctgcc gcgactgtcg tagccccagc accgaaagct gcaacggcaa
    52501 caccagcagc tcctgcacaa cctgggctgt taagccgctt cttcggcgca ctgaaagcgc
    52561 tgttcagcgg tggtgaagaa gccaaaccga ccgagcaacc aacaccgaaa gcagaagcga
    52621 aaccggaacg tcaacaggat cgtcgcaaac ctcgtcagag caaccgccgt gaccgtaatg
    52681 agcgccgcga cacccgtagt gaacgtactg aaggcagcga taatcgcgaa gaaaaccgtc
    52741 gtaatcgtcg ccaggcacag cagcagactg ccgagacgcg tgagagccgt cagcaggctg
    52801 aggtaacgga aaaagcacgt accaccgacg agcagcaagc gccgcgtcgt gaacgtagcc
    52861 gccgccgtaa cgatgataaa cgtcaggcgc aacaagaagc gaaggcgctg aatgttgaag
    52921 agcaatctgt tcaggaaacc gaacaggaag aacgtgtacg tccggttcag ccgcgtcgta
    52981 aacagcgtca gctcaatcag aaagtgcgtt acgagcaaag cgtagccgaa gaagcggtag
    53041 tcgcaccggt ggttgaagaa actgccgctg ccgaaccaat tgttcaggaa gctccagctc
    53101 cacgcacaga actggtgaaa gtcccgctgc cagtcgtagc gcaaactgca ccagaacagc
    53161 aagaagagaa caatgctgat aaccgtgaca acggtggcat gccgcgtcgt tctcgccgct
    53221 cgcctcgtca cctgcgcgtg agtggtcagc gtcgtcgtcg ctatcgtgac gagcgttatc
    53281 caacccagtc gccaatgccg ttgaccgtag cgtgcgcgtc tccggaactg gcctctggca
    53341 aagtctggat ccgctatcca attgtacgtc cgcaagatgt acaagttgaa gagcagcgcg
    53401 aacaggaaga agtacaagtg cagccgatgg tgactggggt ccctgtcgcc gccgctgtcg
    53461 aacctgttgt tagcgcaccg gttgttgaag aagtggctga agttgtagaa gcccccgttc
    53521 agattgccga accgcaaccg gaagtggttg aaacgacgca tcctgaagtg attgccgccg
    53581 cagtaactga acagccgcag gtgattaccg agtctgatgt tgccgtagcc caggaagttg
    53641 cagaacacgc agaaccggtg gttgaaccgc aggaagagac ggcagatatt gaagaagttg
    53701 ccgaaactgc tgaggttgtg gttgctgaac ctgaagttgt tgctcaacct gccgcgccag
    53761 tcgtcgctga agtcgcagca gaagttgaaa cggtagctgc ggttgaacct gaggtcaccg
    53821 ttgagcataa ccacgctacg gtgccgatga cgcgcgctcc ggcaccggaa tatgttccgg
    53881 atgcaccgcg tcacagtgac tggcagcgcc ctacttttgc cttcgaaggt aaaggtgccg
    53941 caggtggtca tacggcaaca catcatgcct ctgccgctcc tgcgcgtccg caacctgttg
    54001 agtaataatt agctcaagta atcaagccct ggtaactgcc agggcttttt tatttcatct
    54061 ttgaatcaac atattaaaat tcatcgaatg gcatccttgc taaccaacaa tgcaaaatag
    54121 gcaaaaaaat gcccgccccg gcgatcccag agcaggcaga caaaacatac ccagtttcat
    54181 gatatgtttt aaagcgaaat tatttgttga gctggaagag cgacaatccc tgcatatcgg
    54241 taaatgcttt atacgatgcc tgcaatgccg tttgctgcat gatgtaagat gaaatagttg
    54301 cattccagtc cacatcaacc agatcgctca tctgctgcgt ttgccctaaa gcgcgatcgc
    54361 tacctaatga atccagcgac tccagttcgt tcagctgcgt gcctaattcc gcgcgaacgg
    54421 tcagcacatt gttcagcgag tttttcagtc cgcggttggt tttatctaac gccgcagcgg
    54481 cggtttcttt atccgcttcg ctatccgcga ccggcgtttt cagggctgcg atggcgctat
    54541 ccagcatggc aaaaagattg gtttcagaag cgctaccgtc tggttccgct accgcgttac
    54601 tggtaatact gtcgaaaatt ttgtcgcccg tatgtcctat caccatcgaa cgcgaagcat
    54661 cgacctgttg cttaatactt tctgttccgc cgacataatc accgttagcc tcactaaacg
    54721 gcgcagtctc tgttttataa ccggcaaaaa tgtagcgccc attaccgtca gtggtgttcg
    54781 ccagattcag caactggtca cgaagcccct gaatatccgt agccagcgag gcccggtcat
    54841 cgtcactcaa ggtgccattg ctggcgtaga caattttttc ctgagcattc tggatagcag
    54901 tggtgacctg gctaagtaca ctctcttcca gtgacacttt ttgagtggcg aaagtacgcg
    54961 ccagcgtgta ctggctgttt tgcgcctgtg cctgggagag aacaaccgct tgtgatgcag
    55021 caatgggatc atctgaaggg ttaacgactc gcttacccgt cgacatctgt tcgccgtact
    55081 tcatccattc tgcctgagaa ttggtgatac cacgcatgtt ttgctggtac atcatctggg
    55141 tactgaaacg cattttatct ccccttagcg aatgttaatc agcgcatcaa aaatcgcgtt
    55201 tgccgtctgc agaacctgcg cattcgccag gtaatactgc tgaaaacgtt gcagatttcc
    55261 gtactcctca tcgagattga caccggaaat cgactgctgc tgattggaaa gctgcgtcac
    55321 cacattacct tgcgtggtgc tactggtttt caacgtcgcg gttttattac cgatatcact
    55381 cactaacgag gcataagcgt cgttaaagga tttcgcaccg cccaccgttt tactattgct
    55441 ttgcagatcc agcagggcct gaccgttgcg gttatcgcta tcacccgcat ctttttcgct
    55501 cgccatcgct attttcgctt cgtcggtgat taatacatcc atgttgacga tggcgtcact
    55561 tactggtttc agcgtgaagc tgtcgttaac ggcaggcgtt cctgtaaacg tcaactccag
    55621 accatcaaat gccactttac cgttggcatc cggcgtcacc gtaaaagtgg tattgctggc
    55681 aaggcgggtg acctgccact gattattatc gaacgagatt ttgtaatctg tcgccagtac
    55741 cacggaggca tcagttaccg tggcaccgat cgcaacgtca cctttgtttt tcgtgttttg
    55801 cagaaccgcg ggcttaccga tagtaaagaa atcttcacca gcatcgccgt tagcatcaaa
    55861 tccggctttg tgttgggtgt tgaaagcctc ggcaaatgcc agcgccagtt gtccaagcgt
    55921 attacgcgtc tggtccagct cctgagaacg gaatgtcaga atgccgccca gcgacccggt
    55981 attcagtaat ttctccggga tctcaatatt gcctgccgtc ccatcaacat aagcgacagt
    56041 cgtacgagaa gggtcagcgc tggaaggaac tgccgccagt tgccgcgccg tacttccctg
    56101 aaccagtgag taaccattgg ccaccgtgat gttataagtg ccgccatcct gaacgctgac
    56161 ttctacacca acaatctggt ttaattcgct caccagttga tcgcgttgat ccagcagatt
    56221 gttaggtgac gcccctgccc ccacgcctgt caggcgcgag atttgatcgt tcaggctggc
    56281 aatttgttta gcgtaattgt tgatctgatc aacgctggca cctatcgcga tattgacctg
    56341 tttgtcctgg tcgcgcagat attgatcggt ggttttaaac tgattcacca acccttctga
    56401 tttcccaatc agcgcctggc gcgctgccgg gtcttccgcg ttactcacca gcgtttgcag
    56461 gctggtgaag aaatcctgca tctgtgttgc cagcgaagag gtactggtgg agagcatatt
    56521 gtcgattttc gacatctgct catagcgggc ggtcaggccg ctactttgcg tctgcgcagc
    56581 acgtaactgg ttggtaataa acgcatcata ctcacgctgc acaccagaaa cgtagacgcc
    56641 attgccaacc cagccgccag cgcccaacgt gctattggcc tgcgccataa tagtggtttg
    56701 gcgggtatat ccggcaacgt tatagctgga gatattatta cttgccgtat ttaacgccgc
    56761 ctgggccgcg ttcagtccgc tcatggcgtt attgatcaag ctggacatgg aggttcctta
    56821 taagccttca attacagagt attatcggca gcgacccgcc ggacttgagt tattcagaac
    56881 agattatcaa tgttcatgct gtaggttttg ctcaccttgt cgcttatcga tttcatctgc
    56941 tggatcatgt tggtgagttt acgggcatag tgaggatcgg tagcataccc cgcgtcctgt
    57001 agggcctgcg ccccctgctc cgcactcgcg gctgtcgtca cggcggcgta gcgcgggtta
    57061 cgtgttaaca gcccaacgta atccgacaat gcttccagat acgagctgta gacccgaaat
    57121 ttcgctttta ctttcttcgc ttcgccattt tcatattcag tcgtggtgat ttcagtaact
    57181 ggacctttcc agttgccaga ggctttgacg ccaaacaggt tatagctcgg ctcgccgttt
    57241 tcgcggcgga tttgccgttg cccccaacca gactccagcg ccgcctgagc gaggatcaaa
    57301 tgatgtggca caccgctttg ctggcttgcc agttgggcgg gcagcgagag ttgcgcgagg
    57361 aatgctttac tgtcacccgg cagcgaatca tcgtagttac gtggcacggc cttttgcacc
    57421 agctgcgaaa gcgcctgatt ttgataacgc accacagttt cgagcgggaa tttcatcggt
    57481 gctgctggcg tggactcctc tggcaatggt tgttctggcg tcatctgttt aaccatcatc
    57541 tctgcaagcc ccagaccttt gcccgccgtc atctgttggg caatctgctg gtcatacata
    57601 ctggtataca ggcgagtgtg ctcgctgctg aacaggccat cttttggtaa agcgtcgcgc
    57661 atgcttttca acatcatctg cacgaacatc ccttccacct gacgggccac cggacggata
    57721 tttgccgccg gatcttcgcc cgctttcgcc tttagttcgt tgagtgattg cgcatcccag
    57781 gccgcacttg ccagtagttt gctgtcgctg atcatcagat gatttccagt tttgcccgca
    57841 gacatcccgc actttgcatt gattgcagta tggacatcag atccatcggc gtagcgccca
    57901 gcgcattgag cgcgcgcacc acgttattga ggctggcgct ggaacgtacg ctttgcagcg
    57961 aaccgccgct ctggcgtaaa tcgatctgcg tttgtggagt aaccacagtc tgtccaccac
    58021 caaacggtgt atctggctgg ctgacattgg cctgacgatt gactgttact gagagatttc
    58081 cctgcgctac cgcgcagctg tcgagggtca cttcgcgatt catcaccacc gaaccggtgc
    58141 gcgagttaat cactacttta gcgtcctgcg gggtgacatt aacctgcata ttctggatat
    58201 ctgcaaggaa acggacctgg gaactgttgc cacttggtac gcgcacctga atagtccgcg
    58261 catctaacgc ggtggcgctg ccatatccac gcacgcggtt gatggtgtca gcgatttgct
    58321 gcgccatgct gaaatcttcg tcgttaagtt gcaaattaag ggtattcccg acgccaaact
    58381 ggctgggcaa ttcacgttca ataaccgcac cattggtgat ccgtccaccg ttcagttggt
    58441 tcacctgaac actgctaccg ccagcggagg ctcctgcgcc gccaaccaga atattgccct
    58501 gcgccagcgc atacacctga ctgtcaacgc ccttaagcgg cgtcatcaac aacgtacctc
    58561 cacgcaagct tttggcattt cccatggaag aaaccaccac atcgatggtt tgcccctgac
    58621 gtccaaacgg aggaagtgac tctgtcacca ttaccgcagc gacgtttttt agctgcatat
    58681 tggtgcccgt cggaacggta attcccagct gtgagagcat gttattaagc gtttgtgtgg
    58741 taaacggcgt ctgggttgtc tggtcaccgg tgccatccag ccccaccacc agaccatagc
    58801 caatcagtga gttttgcctt accccctgaa cactggtgag atcgcgaata cgctcagcct
    58861 gagccgccgt cgtaaccagt agaagaatta atgcagagag aaatttaatc accacagcct
    58921 cacttacatt ggcgacaggt taaggaagaa acgctgcaac cagcccatat tttgcgcttc
    58981 gttaatgtag ccattgccta cgtattcaat gcgcgcatcc gccacctgag tagacggtac
    59041 ggtattgctg ccgctgatag tgcgtggatt aaccacgcca gagaagcgaa taaattcggt
    59101 accctgatta atggcaatct gtttttcacc caccacatgc aggttgccgt tgaccagtac
    59161 ctggtcaacc gtcaccgtca acgtgccgct aaaggtattg ctggcattgg ccccgccctt
    59221 tccgttgaac gtgttaccac cggaggcttc gacatcggca cgagcgttac caaacagccc
    59281 ctgcaaatag cgcggcacag tatcaaagcc aaaattagtt ttaccgtcac ggctggcatt
    59341 cgcagaggag cttttgctgg cgctgacgtt ctcctgcaac acgatggtca gcgtatcgcc
    59401 aatattgcgt ggtcgacgat cttcaaacag cggttgatag ccatagttaa tcggctgagc
    59461 agactggaaa atagaaccgt tggcgacggg cgtcggaccg ggaaccggtt gtgcactggt
    59521 cgccccctgc accagcggcg tggagggtat ccaggcgcag ccggttagtg aaagcaccaa
    59581 caagctggaa atggcataag tatgcgcagc gtttttttgc atggctatca tcttcaaaaa
    59641 tcagtaaacc ggtgaacctg ccaccggtta agccttagag ttgcgtcagt ttttgcagca
    59701 tctgatcggt ggtggacacc gctttactgt tgatttcgta agcgcgttgc acctgaatca
    59761 tattgaccag ttcttccgcc acgttgacgt tagacgtttc aacataccct tgatacagca
    59821 gtcccgcgcc gttcaggccc ggcgtgcttt cgttcggtac accagaggat tgcgtttcgg
    59881 tgtagaggtt ttcgccaatg ctctccagcc cggtgtcatt cataaaggtg gtgagattga
    59941 gctgcccaac ctgaaccgga gctgcctggc cttgttgggt tacgctgacc acgccatcac
    60001 gaccgatggt gatacttaac gcattcgccg gaatggtgat cgctggctgc acctgaaaac
    60061 caccagctgc ccgttctgat ccacctggaa agagccgtca cgggtatagg ctgatgaacc
    60121 atctggcaac atcacctgga aaaagccctg ccctttaatc gcgacatctt tgctgttgtt
    60181 ggtctgcgac aggtttccct ggctgtgtaa gcgttcagtg gcgaccgggc gtacccccgt
    60241 gccgatttgt aatccggagg gtaaggtggt ttgttcggaa gactgtgccc ccggctggcg
    60301 aatggtttga taaagcagat cttcaaacac cgcgcgctga cgcttaaaac cgttagtact
    60361 gacgtttgcc aggttgttgg caatgacgtc catattggtt tgctgggcgt caaggcccgt
    60421 tttggcgatc cataatgaac tgatcataaa atgtcctgta gtggattagt gacaaagtgc
    60481 atatgtcttc ttgccggatg cggcgtaaac gccttatccg gcctacaagt gctcgcaaaa
    60541 tcgataaatt gcgattgccc ccatagaccc gataaacttg cgcatcgggc aacttatact
    60601 tgtcatgtat cctttcaatt aactcatcga cagcagttgg ttggcacggc ctgcgttatc
    60661 atcgacgctg ctgatcacct tcatctgcat ttcaaaacgc cgcgcgctgg caatcatgtc
    60721 gctcattgcc gcaacggcat tgacgttact gccttccaga acccccgaca tcacacgcaa
    60781 ggttggatct gcctgcagta ccggccctcg cgtggcctgg gtttctgcgc ttaaacgaaa
    60841 aatgccgtcg tcaccgcgct gcacttcgct gcccgtggct ttcaccagtt tcagacgccc
    60901 tactggcgca actgtatttg ccggatcgcc cggattgagc gccgagattg tgccatcggc
    60961 agcaatagtg atttccgctc cttccggcac agcaattggc cccgcctcgc ctatcacctg
    61021 atgcccctga attgtcagtt gcccggtggg atcaacctga atgctgccat tacgcgtata
    61081 cccttcgctg ccgtcagcgg tctgcacggc cagccagcca tcctgctgca acgcgacgtc
    61141 cagcgggcgc gaggtgtaat ccattttgcc gggcgtcata tctgcgcccg gcgttgacgc
    61201 cgtgaccaac gtgcgcgtgg gcagagaaag cccttccact ggcaccgcgc gtaaagcatt
    61261 caactgcgcg cgaaaaccgg gcgttgaggc attggccaga ttactggcgg ttaccgcctg
    61321 ttgattcagt gtctggctgg ctgctcccat cgcggtataa attgcgtgat ccattgagct
    61381 atcccgtcag cgattagcgt aagttaacca gcgtgttgag gatctggtcc tgagttttga
    61441 tggtctgggc gttagactga tagttacgct gggcaacgat catattgacc agttctttac
    61501 tgagatcgac attggacgct tccaacgcac cgttggtcag ggtgccaaag ttgcccgtcc
    61561 cggctgtccc caacagcgcc acgccagaag attgcgtcgc agaccagacg ttgtcgcctt
    61621 cggatgccag accttcgttg ttggcaaagt tcgccagtac aatctgcccc agcagttgtg
    61681 tttgttcgtt ggaatagttg ccgacaaccg taccgtcatc attgatttga taactcacca
    61741 gatcgcccgg tttgtagcca ttctgggtgg ttgccacaat attgttagcg ccggtatttt
    61801 gctgcatgga gttgaggaag ctcaggctaa acgtggcggg atctgcgccg ttaattgcgc
    61861 cagtggtaat attctctgtt ggatttgagg tcagaacgcc attggcatta aacaccagct
    61921 tcattgcagg ctcggctgta cctgttggat cactgctatc ctgggtgtag acctgccagt
    61981 tattatcccc ggtcttcaca aagtagacgc tcatgtcatg agcattaccc tgactgtcga
    62041 aaacagtcac cgaacctttt ttgttatagc tatccgcatt gctggcgctg aatggcgtaa
    62101 cagtaggaag cggatcactg gaattcaggt tgatctgcat cgacgccgtg gtggtagttt
    62161 tcgctgccat cagggtattc gggatcgaaa tattggtcgg attcgcccct tgctgaatag
    62221 tcggcggcgt accggttgcc gggtaacccg tcagctgtaa accttgcata ttcaccaggt
    62281 tacggttttc atccagctta aattgtccgt tacggctgta gaacaccgaa ccgttgctgt
    62341 ctaccagacg gaaaaaaccg ttctggctga tagcaacgtc cagacctcgc ccggtgttgg
    62401 tggtcgtgcc atcggtaaag tcctgagtga taccggcaac ttttaccccc agtcccactt
    62461 tcgaaccggc aaacatatcg gcaaaagagg ccgtgcctga tttaaagccg taggtggcgg
    62521 agttggcgat attgttgcca ataacatcga ggttggtggc ggcagcgttt aatccgctaa
    62581 ccgcttgaga aaaggccatg actgactcct gaaatgtgaa ggcttaaatt atctgccgta
    62641 cttcgtcgag ggtggtggtg ccgtaagtgc cgagatccag cgtattaccg ctgttgccgc
    62701 ggagcacacc ctgcaccaga gcaaactgca gcggctgggc aaccagttgt gtaccaccgt
    62761 tactggcgct aatcgctaca ttgtaagaac cgttcggcgc agtgctgcca tcagtcaacg
    62821 taccgtccca ggtgaaactg tggactccgg cggtcagttc accaatatca atggtgcgca
    62881 caaccgcgcc atttttatcg gtgatggtgg ccgtaacttt gtctgccgcc tgttgcagct
    62941 caacaccaaa cggcgtggtc gtggtcacag ccccttcttc actgccggtt cccgcaagaa
    63001 cagtggtgcc ggggatcatc acgccgtgac cgatcaggtt actggcctgt aacgactggc
    63061 tgttatcaat ctgtccggaa atagatccga gcgtggtatt gagtttttca atcccgctga
    63121 ccgtgctgat ttgtgccaat tgcgacgtca gctcgttgtt ttccattgga ttggtcgggt
    63181 cctggttttt cagctgcgcc accaacaaag tcagaaaact gctttgtaag tctgcggcgt
    63241 tgctgcccgt gagcgaacta ctgctggtgg tactgacgcc ggtatttgtc ggatcggtgg
    63301 tggttaccgc aatggacata gctttctcct ttattgaccg agcgtaaggg ttttcagcat
    63361 catgcttttc accgtgttga gcacttcaac attggcctga tagctgcgtg acgccgacat
    63421 ggtgttaacc atctccccga cgacatcaac gttcggcatt tttacgtagc cctttgcatc
    63481 cgccagcgga ttacccggtt cataaaccag tttgtccggg gcctgacttt ctataacatc
    63541 ggcaaccttt acgccgcctg tcgcagcacc tggtgcagcg ttaacctgga ataccacctg
    63601 ttttgcccga tatggctgtc catcgggacc ggtcacgcta tcagcattcg ccagattact
    63661 ggccgccacg ttcaagcgct gggactgggc agttaacgcc gacccggcga tatcaaaaat
    63721 attcagcagt gccatccgtt aatttccgct ctgtaaaacg ttcatcatgc ctttgatttg
    63781 cccgctcaac gcgctaaggc tcatctggta ttgcaggctg ttatcggcaa actgggtgcg
    63841 ttcgcgatcc atatcgacgg tattaccgtc aagcgaaggc tggtccggaa tacggtattg
    63901 cagttctgcg gtaggaggcg tcagcgcctg cgccggaatg tgttgcgttg aggtcatcgt
    63961 cagtgcaacc acactggttg catcccgtcc acgttgcatg acttttttaa gttcactggc
    64021 aaaatcgata tcgcgcgcct gataaccagg ggtatcggca ttggcgatgt ttgctgccag
    64081 cacttcctga cgctgggcgc gcagattgag cgcctcttgt tgaaaacgta aggcggcgtc
    64141 gagcttatcg agcatatctc ctccgcaggt atcaaaattc tgccatcagc ttaaatgcct
    64201 ttacgcgcgc gttatcggcg gaataaacgc aaaatgggtc gctatttatg ccgttgatga
    64261 ccattgcgga caggtacaat tcacgttgta gaaatggctg ggggcgaaaa tgctggcaat
    64321 aaaacgtagc gtggcgatca tctcgatact attcactccg ttaagtgcgg cgagtaatct
    64381 cacatcgcaa ttgcaaacct tttttagcgc ccagctcgcg ggggtaagtg atgaggttcg
    64441 tgtttctatt cgtacagcgc ccaatctact accgccgtgc gagcagccat tgctttcgat
    64501 gagcaataat tcccgcctgt ggggcaatgt gaatgtgttg gcacgctgcg gtaacgacaa
    64561 acgatattta caggttaatg tacaggccac aggaaattat gtggttgccg cgatgcccat
    64621 tgtgcgggga ggaaagctgg aagctggcaa tgtcaaacta aaacgcggac ggcaggatac
    64681 cctgccaccg cgtacggtgc tggatatcaa tcaacttgtt gatgccgtta gcctgcgcga
    64741 tctatcaccc gatcaaccta tccagttaac ccagtttcgc caggcatggc gggtaaaagc
    64801 gggacaacgc gtcaacgtga tcgccagcgg tgacgggttt agcgccaacg cagaaggtca
    64861 ggcgctgaac aatgcagccg tcacacagaa tgcgcgggtg cgcatggtat cgggactggt
    64921 ggtcagcggc gttgttgatg cagatgggaa tattcttata aaccagtaag ctgttaaaga
    64981 ttacccgtcc cttgccgata aataagcaac acatgataaa agcgccctca atgaggaata
    65041 aaccatgagt attgatcgca cttcgcctct gaagcccgta agcaccgttc aaccacgcga
    65101 aaccactgac gcgtcggtaa cgaacacccg ggcggcaaaa acaacagcct ccaccagcac
    65161 cagtgtgacg ttaagcgacg cgcaagcaaa actgatgcaa cccggcagca gtgatatcaa
    65221 tcttgaacgt gtcgaagcgt taaaactggc gattcgtaac ggtgaactaa aaatggacac
    65281 cggcaaaatt gccgatgcgc tgctcaacga agcgcagcaa gacttgcaga gtaactgatc
    65341 gtatgacacg tcttgcagag atcctcgacc agatgtccgc tgtgcttaac gatctcaaaa
    65401 cggtaatgga tcaagaacag caacatctct ctatggggca gatcaacggc agccagttgc
    65461 aatggattac agaacaaaaa agctcactgc tggcgacgct ggattacctc gaacagttac
    65521 gcaggaaaga acccaataca gcaaatagcg ttgatattag tcaacgctgg caggaaatta
    65581 ctgtgaaaac gcagcaacta cgccaaatga atcaacataa cggctggtta ctggaaggac
    65641 agattgagcg caatcaacag gcgctggaaa tgttgaaacc gcatcaggaa ccgacgctat
    65701 atggggcgaa cggtcagacc tcaacaaccc atcgcggcgg taaaaagatt tcgatctgaa
    65761 tattatctcc ggcctgcact gcaggccgga atgcattgtt acaccgtccg gcgggcaaat
    65821 tctttaactt tgaagcccag taccgccagt gcagcgaagt acgcggcaat ccccgccagc
    65881 acgaccgcca ttaaacgcag taagcgccag ggcatggtac ccaatgacca ctccggcatg
    65941 atatgtaaca tacctaaaag cacgccagac atcaccagta ccgccaccac cagacgcaac
    66001 agaaacgcca tccagccggg ttgcggagta aagattttct gcttacgcaa ctgccagtaa
    66061 agcagcgaag cattcagaca cgccgccaga ccaatagaaa gtgacagccc agcatgtttc
    66121 aacggaccaa taaacgccag gttcatcaat tgcgttaaaa tcagcgtaac gatggcaatt
    66181 ttcactggcg ttttaatgtc ctggcgggaa taaaagccag gagccaacac tttcactaca
    66241 atcaggccaa tcaaacccac cgagtaggca attaacgccc gctgggtcat cagcgcatca
    66301 aacgcggtaa atttaccgta ctggaacagc gaaacggtta acggacccga aagaatgccc
    66361 aacgcaaccg cactcggcag cgccaacagg aaacaaagac gcaaccccca gtccatcaaa
    66421 cggttgtatt catcatgatt gccactggca aaacttttcg acagcgacgg cagcaaaatg
    66481 gtgccaagcg ccacgcccag cacaccggag ggaaactcca ttaagcggtc ggcgtaatac
    66541 atccaagaca ccgaaccgga agcaagaaac gaggcaaaaa tggtgttgat gattaaggag
    66601 atctggctca cagagacgcc aaggatcgcc ggtcccatct gtttcaccac gcgcattgct
    66661 ccggcatcgt ggaagttaat gcgcggcagg accagcatgc cgatcttctt caggtgcggt
    66721 agctgataca ccagctgcag gatgccgccg accgtaacag cccacgccag cgccagcacc
    66781 ggtgggttaa agtacggtgc ggcaaacagc gcgaaaccaa tcatgctgat gttaagcagt
    66841 gttggcgcaa acgccggaat cgagaagcgg ttccacgtat tcagaatcgc tcccaccagc
    66901 gacgccaggg agatcagcaa gatataggga aaggtaatct ttagtagctg gctggtcagg
    66961 gcaaatttgt cagctgtgtc agcgaagcct ggcgcggtca ccatgatcac ccacggtgcg
    67021 gcgagcatgc cagcgaccgt cacaaccgcc agcgcaagtg tcagcaggcc agaaacataa
    67081 gagacaaaga cccgcgtggc gtcttcaccc tgcttacttt tatattccgc cagaatcggt
    67141 acaaatgcct gggaaaatgc cccttcggca aagatacggc gtaacaagtt aggaagttta
    67201 aaagcgacga aaaaggcgtc ggttgccatc cctgcgccaa agattctggc gacaattgcg
    67261 tctcgtgcga agccaagcac acgcgaaaac atggtcatcg agctgacggc ggccagcgat
    67321 tttaataaat tcatcggtgt tctaatccag acccacgggc aaaacgcctg caaggcaggc
    67381 gctgaaagag gcgaatagtc tacctggatt atggtgaatt gctaccgcca gatgttacag
    67441 ggtttattca ctcatcgcat cgcgccagat cttgtcaacg atacgttgcg ccagcactgc
    67501 ctgttcgccg gcggtttgca gaactgtctg attttgcaca cattcaatga agtgccgcgc
    67561 acagccgaca aatccacgtt gctcaagagt gctctgccaa ccaggaatcg gtttatgcac
    67621 tacgccctgc ccgcgctcct cacgccattc gcgcatatcc gtaatgtcga tgagcgcatc
    67681 gtcagtcacg gcctgcacgg tttcacgctg acttccggca cggcgatgca tacaggtggt
    67741 gatctgcaaa ggatcggccg aaaaatggtg ctcggcaaac agcatttcgc cagcgtcgtt
    67801 agtcagtagc gtaccgccat ccagagaggc tttgccgccc gacaaccaca gcgcggtatc
    67861 caccacatgc agataatcat ccagcaacgt gaaataaaga tcgtgtggcc cgacactatt
    67921 gctacgatgt ttatccattc ttagcgaagc tgcggtagcg agttgcgttt ttaactcacc
    67981 gtagagtggt gcgaaacgac ggttaaaacc gaccatcaac gtcagttttt tccgcgccgc
    68041 cagttccacc agccgttcag catcgcgcag attttctgcc agcggtttat cgacacagac
    68101 atgtacaccc gcattgagta acgtactgac cacgtcaaag tggctggcgg tgctggaatg
    68161 cacaaaaacc gcatcgcaac tggcggcaag gctggataac gaatcggcat aaggaatgcg
    68221 ccagctttca caaattggca gggctttcgc gcgcgtaggc gaccaggctc cttgtaacat
    68281 ccagtcagac gctgccgcca gcaccggtaa ccacgctttt tgcgcaatgc cgcctaatcc
    68341 cacaacgccg atacgtaatt ttttcacttt aatctcccag atgggccagc aacgaatcaa
    68401 gacgctgttt cagttctgcc acttcgattt ccagggcttc gacgcgggcc tgtaaatcac
    68461 catcaaccgc gttcgacata tccgttaccg ccggctgatc ctctacctca ccactgaaaa
    68521 gatgcatgta gcggctttcg cgtttacccg gttcgcgggc cagacgcacc acaaaaggac
    68581 catcttcgcg atttgccagt tgttccagcg tcgactccac ttccgccata tcgctgaatt
    68641 catacattcg cgcggcgcgg ctgcgcagtt cacccggcgt ctgggcacca cgcaataaca
    68701 acgtggtgat taacgccact tccgctgcgc tcagtttcag atcgccaaat tctgaattac
    68761 aaaaacgttg ttcatatttg gtgacccgat taccaaagcc actcaccgtg cgcagataat
    68821 ggcgtttgac cagattatcc agttgctcct gcacttcaga ttcactcaga ttcatgaccg
    68881 gttcacggtt cgttttctga ttacaggccg tgactacacc attgactgag agcgggtatt
    68941 gctccggcgt ggtcacctgc ttttccagca agcaaccaat cacgcgagct tcaagggcag
    69001 taagttgata tttcatgaga ttctccttag cggccgggcg tccagtctgg ggtagttaat
    69061 gccgtcagta cgtgatcgcg ccattgtcca tcaatcaaca gatagtcttt cgcatagcct
    69121 tctttttcaa aacccagtcg cgccagtaaa tcaccgctgc gtttattgtg cggcatataa
    69181 ttagccataa tgcgatgaat atgttgggtg cgctgcatat aacgaatggc tgcggtcagg
    69241 gcttcaaaca tgagtccttt gccctgccat ttttgcccaa tcgaataacc gagatagcag
    69301 gcatgaaaag agccacgaac aacattggaa aaattggcaa cgccaataat ctctttttcg
    69361 tccgggtcga ataagccaaa gtagaaagct gaaccttgtt tatgaaattc gttaatcatc
    69421 cccagcctgg cctgccagcc tgatggataa cagtggcttt cgtcgcgcac tggctcccag
    69481 ggcttgagga aatggcgatt ctctgcgtaa taatccgcaa gacgccaggc atcacgatca
    69541 tgcaccagac gcacgaccag tcggtctgtg gttaagcgca cttttggcac gttactgcga
    69601 tagccaaaca ttctatacct actccttccc gtaacgtctt taatacgctg taagtattac
    69661 tatacctgcg caaccagacc ctgtgaaaag tccaaaatgc cattttctgc attattcaca
    69721 tatttgataa aaactctcta tcaaagcgat gttttgataa aaaaatattg tccccgaagt
    69781 gatgggaaaa gtcttagcgg caatgcagaa tagaaagtgc tcatctttta tttcccggga
    69841 ggggaaatgt cccgcgtatc gcaggcgagg aacctgggta aatatttcct gctcatcgat
    69901 aatatgctgg tcgtgctggg gttctttgtt gtcttcccgc tgatctctat ccgcttcgtt
    69961 gatcaaatgg gctgggccgc cgtcatggtc ggtattgctc tcggtctacg ccagtttatt
    70021 cagcaaggtc tgggtatttt cggcggcgca attgccgacc gctttggtgc caaaccgatg
    70081 attgttaccg gtatgctgat gcgcgccgcc ggattcgcca caatgggtat cgcccacgaa
    70141 ccgtggctat tgtggttttc atgcctgctc tcgggactcg gcggcacgtt gtttgatccg
    70201 ccgcgttcgg cgctggtggt gaaactaatc cgtccacaac aacgttgtcg ttttttctcg
    70261 ctgttgatga tgcaggacag tgccggtgcg gtcattggcg cattgttggg gagctggctg
    70321 ttgcaatacg actttcgcct ggtctgcgcc acaggggccg ttttgtttgt gctgtgtgcg
    70381 gcgttcaatg cgtggttgtt accggcatgg aaactctcca ccgtacgcac gcccgttcgc
    70441 gaaggcatga cccgcgtgat gcgtgacaag cgttttgtca cctatgtcct gacgctggcg
    70501 ggttactaca tgctggctgt acaagtgatg ctgatgctgc caattatggt caacgacgtg
    70561 gctggcgcgc cctctgccgt taaatggatg tatgccattg aagcgtgtct gtcgttaacg
    70621 ttgctctacc ctatcgcccg ctggagtgaa aagcattttc gtctggaaca ccggttgatg
    70681 gctgggctgt tgataatgtc attaagcatg atgccggtgg gcatggtcag cggtctgcaa
    70741 caacttttca ccctgatttg tctgttttat atcgggtcga tcattgccga gcctgcgcgt
    70801 gaaaccttaa gtgctttgct ggcggacgca agagctcgcg gcagctatat ggggtttagc
    70861 cgtctgggtc tggcgattgg cggcgctatt ggttatatcg gtggtggctg gctgtttgac
    70921 ctgggcaaat cggcgcacca gccagagctt ccgtggatga tgctgggaat tattggcatc
    70981 ttcactttcc ttgcgctggg ttggcagttt agtcagaaac gcgcaacgcg tcgtttgctg
    71041 gaacgcgacg cctgatttga tactcccccc tgactcctcc atactgaaag tgaactgaca
    71101 gccaaatgga ggagtcacgt gaagctatac atttacgatc actgccctta ctgcctcaaa
    71161 gcccgcatga ttttcggcct gaaaaatatc cccgtcgaat tacatgttct gctcaacgac
    71221 gacgcagaaa cacccacccg gatggtcggt caaaaacagg ttcccattct gcaaaaagat
    71281 gacagccgct atatgccaga aagcatggat atcgttcact atgtcgataa actcgacggc
    71341 aaaccgttac tgaccggcaa acgttcccct gccattgaag agtggctgcg caaggtcaat
    71401 ggctacgcca acaaactgct gttgccgcgt tttgccaaat cggcatttga tgagttttct
    71461 actcccgccg cgcgcaaata tttcgtcgac aagaaagagg ccagcgcggg taattttgcc
    71521 gacctgctgg cccactctga cggtctgatt aagaatatca gcgatgattt gcgtgcgctg
    71581 gacaaactga tcgtcaaacc gaacgccgtg aatggcgaac tttcggaaga tgatattcag
    71641 ctatttccgc tactgcgtaa tctgacgctg gtagccggaa tcaactggcc aagccgcgtt
    71701 gctgattacc gcgataatat ggcgaaacag acacaaatca atttgttatc atcaatggcg
    71761 atttaatcct gacccgccgc ggcagcctgt attcgcgggt tgccgccgtt ttcagttttt
    71821 tctgctggcg agtttatcgc tggtgacaca tgctgcgttg tgtcaggata ttgacgcaga
    71881 tttcatgtcg aggactacca tgaacaagtt tttatttgcc gctgcattga tcgtcagtgg
    71941 cctgctcgtt ggctgtaatc aactcaccca atacaccatc accgaacaag aaattaacca
    72001 gtcgcttgcg aaacataata atttctcaaa agatatcggt ttacccggcg tggctgacgc
    72061 ccatattgtt ctgacaaact tgatcagcca aattggtcgc gaagagccga ataaggttac
    72121 cctaaccgga gacgccaatc tggacatgaa ctccctgttc ggtagtcaga aagcgaccat
    72181 gaaactgaag ctgaaagcgc tgcccgtgtt tgataaagaa aaaggtgcga tcttcctgaa
    72241 agagatggaa gtggtcgatg cgacggtaca accggaaaaa atgcaaacgg tgatgcaaac
    72301 gttgcttccc tatttgaacc aggcattacg caattacttt aaccagcaac ctgcttacgt
    72361 cctgcgcgaa gatggcagtc agggcgaagc aatggcgaaa aaactggcga aaggcattga
    72421 agtgaagcca ggcgaaattg tcattccgtt tacggattaa tgacgagggc gcattcgcgc
    72481 cctttatttt tcgtgcaaag gaaaacgttt ccgcttatcc tttgtgtccg gcaaaaacat
    72541 tccttcagcc ggagcataga gattaatgac tgcaccatcc caggtattaa agatccgccg
    72601 cccagacgac tggcaccttc acctccgcga tggcgacatg ttaaaaactg tcgtgccgta
    72661 taccagcgaa atttatggac gggctatcgt aatgcccaat ctggctccgc ccgtgaccac
    72721 agttgaggct gccgtggcgt atcgccaacg tattcttgac gccgtacctg ccgggcacga
    72781 tttcacccca ttgatgacct gttatttaac agattcgctg gatcctaatg agctggagcg
    72841 cggatttaat gaaggcgtgt tcaccgctgc aaaactttac ccggcaaacg caaccactaa
    72901 ctccagccac ggcgtgacgt caattgacgc aatcatgccg gtacttgagc gcatggaaaa
    72961 aatcggtatg ccgctactgg tgcatggtga agtgacacat gcagatatcg acatttttga
    73021 tcgtgaagcg cgctttatag aaagcgtgat ggaacctctg cgccagcgcc tgactgcgct
    73081 gaaagtcgtt tttgagcaca tcaccaccaa agatgctgcc gactatgtcc gtgacggaaa
    73141 tgaacggctg gctgccacca tcactccgca gcatctgatg tttaaccgca accatatgct
    73201 ggttggtggc gtgcgtccgc acctgtattg tttacccatc ctcaaacgca atattcacca
    73261 acaggcattg cgtgaactgg tcgccagcgg ttttaatcga gtattcctcg gtacggattc
    73321 tgcgccacat gcacgtcatc gcaaagagag cagttgcggc tgcgcgggct gcttcaacgc
    73381 cccaaccgcg ctgggcagtt acgctaccgt ctttgaagag atgaatgctc tgcagcactt
    73441 tgaagcattc tgttctgtaa acggcccgca gttctatggg ttgccggtca acgacacatt
    73501 catcgaactg gtacgtgaag agcaacaggt tgctgaaagc atcgcactga ctgatgacac
    73561 cctggtgcca ttcctcgccg gggaaacggt acgctggtcc gttaaacaat aaaaatcgta
    73621 gcttcctgtt gtcattaggt tatttaacct gtataaataa ccagtatatt caacaggggg
    73681 ctattatgcg aattgaagtc accatagcga aaacttctcc attgccagct ggggctattg
    73741 acgccctggc tggcgaactt tcccgccgta ttcagtatgc gtttcctgat aatgaaggcc
    73801 acgtatcggt acgttatgcc gcagcgaata atttatcggt tattggcgca acaaaagaag
    73861 ataaacagcg cattagcgaa attctccagg aaacgtggga aagcgccgat gactggtttg
    73921 tcagcgaata atatgcagtg atttttgttt ttgccgggtc gccccggctt ttttatttta
    73981 cttacgaatt ttgagtacaa aatttctaca cttcctcaaa ataattgcac aatatgctaa
    74041 aaaaatatga atgacatgct gttttattgt tcatataaat cttaaaagca gaaatttatc
    74101 tgttcccccc tttaaaaatt cggtgaatac ccttacttat tggtatactg aagttgcttc
    74161 agataaaaac gcattgaacc tcgaataacg ttgtctagta acacgaatta gggggccatg
    74221 atggaaaaaa ataatgaagt cattcagact catccgctcg tagggtggga catcagcacc
    74281 gttgatagct atgatgcgct gatgttgcgt ttgcactacc agaccccaaa taagtccgag
    74341 caggaaggga ctgaagttgg tcaaacgctc tggttaacca ctgatgttgc cagacaattt
    74401 atttcgatat tagaagcagg aatcgccaaa attgaatccg gtgatttcca ggtaaacgag
    74461 tatcggcgtc attaacatga catgaccatc cacccaaaag gcacctcacc ggtgccttta
    74521 ccatttctgg gttgtatatc tgcattcagt taattactct gcttaaacca tgagagcaga
    74581 gagaaaaaaa tgaaatacga tctcatcatt attggcagcg gttccgtagg cgctgccgcc
    74641 gggtattatg caacccgcgc cggtttaaac gtgctaatga ccgacgccca tatgccaccg
    74701 catcaacacg gcagccacca cggcgatacg cgattaattc gccatgctta tggtgaaggc
    74761 gaaaagtatg tcccgctggt cctccgcgcg caaacgctgt gggatgaact ttcccgccac
    74821 aacgaagatg atcccatttt tgtacgctct ggtgtcatta acctcggccc ggctgactcc
    74881 gcatttctcg ccaacgtcgc ccacagcgcc gaacagtggc aactcaacgt tgaaaagctc
    74941 gatgcgcaag ggattatggc ccgctggcca gaaatacgcg tcccggacaa ctacatcggc
    75001 ttatttgaga ctgattccgg ttttttgcgc agcgaactgg cgattaaaac ctggatccaa
    75061 ctggcgaagg aagcgggctg tgcgcaactg ttcaactgcc cggtcaccgc aattcgtcat
    75121 gacgatgatg gcgtaactat tgaaacggtt gacggtgagt atcaggcgaa aaaagcgatt
    75181 gtctgcgcgg gaacatgggt aaaagacctg ctcccggagc tgcctgtcca gcccgtacgt
    75241 aaagtatttg cctggtatca ggccgatggc cgctatagcg tgaagaataa attcccggcg
    75301 tttaccggtg aactgcccaa tggcgatcaa tattatggtt ttccggcaga aaacgacgcg
    75361 ttgaagattg gcaaacataa cggaggccag gttatccatt cagcggatga acgtgttccg
    75421 tttgcggaag tggtcagcga tggttcggaa gccttcccgt tcttgcgcaa tgtattgccg
    75481 ggtatcggtt gctgcctgta cggcgctgcc tgcacctatg ataattcgcc tgacgaagat
    75541 tttattatcg ataccctacc cggccacgat aatacactgc tcattaccgg cctgagtggg
    75601 cacggtttta aatttgcgtc agttttaggg gaaatagctg ccgattttgc gcaagacaaa
    75661 aaaagcgatt ttgatttgac gccattcagg ctttcccgct tccaataatc atcattacgg
    75721 cctccagaat gaggccgttt ttacaggagt atttaatgcg tcggctgttg cactatctca
//
LOCUS       DAEEKQ010000004        74293 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.004, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000004 DAEEKQ010000000
VERSION     DAEEKQ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 74293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 74293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..74293
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.004"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..941
                     /locus_tag="KLK57_000246"
     CDS             <1..941
                     /locus_tag="KLK57_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE0890735.1"
                     /translation="MLGEKRLVSSVTKEDLLFVRRDLLTGYQKLSNGKISSIKGRSVV
                     TVNYYMTTIAGMFQFATDNGYTSGNPFNGLTPLKKSKIEPDPLTRDEFIRFIEACRHQ
                     QTKNLWIIAVYTGIRHGELVSLAWEDIDLKARTITIRRNYTKLGEFTPPKTDAGTGRT
                     IHLVQPAIDALKSQAEMTMLGKQHSVEVKQREYGRSTVHKCTFVFSPQVIKQRQFSGP
                     HYKVDSIRESWTSILKRAGLRHRKSYQSRHTYACWSLAAGANPSFIASQMGHTNAQMV
                     FNVYGAWMKDNNHEQIELLNKRLSESVPCMPHKKVG"
     gene            977..1663
                     /gene="mlrA"
                     /locus_tag="KLK57_000247"
     CDS             977..1663
                     /gene="mlrA"
                     /locus_tag="KLK57_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HBE0890736.1"
                     /translation="MPVTLRAWQRRYGLLKPQRTDGGHRLFNDADIDRIREIKRWIDN
                     GVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRTWIKERGQDYPAQTLT
                     THLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKKQGKDALVVGWNIQDT
                     TRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGENRTSAQQQQLTSWQE
                     QGHDIFPLGI"
     gene            1723..1830
                     /locus_tag="KLK57_000248"
     CDS             1723..1830
                     /locus_tag="KLK57_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HBE0890737.1"
                     /translation="MRIAKIGVIALFLFMALGGIGGVMLAGYTFILRAG"
     gene            complement(1811..2542)
                     /gene="yehW"
                     /locus_tag="KLK57_000249"
     CDS             complement(1811..2542)
                     /gene="yehW"
                     /locus_tag="KLK57_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HBE0890738.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(2547..3473)
                     /gene="yehX"
                     /locus_tag="KLK57_000250"
     CDS             complement(2547..3473)
                     /gene="yehX"
                     /locus_tag="KLK57_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HBE0890739.1"
                     /translation="MIEFGHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSSSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLEEA"
     gene            complement(3466..4623)
                     /gene="yehY"
                     /locus_tag="KLK57_000251"
     CDS             complement(3466..4623)
                     /gene="yehY"
                     /locus_tag="KLK57_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HBE0890740.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGTLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            complement(4630..5547)
                     /gene="osmF"
                     /locus_tag="KLK57_000252"
     CDS             complement(4630..5547)
                     /gene="osmF"
                     /locus_tag="KLK57_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HBE0890741.1"
                     /translation="MPLSKVWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDAEQNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLKEGGTF
                     KLAASAEFIERADALPAFEKAYDFKLDQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPKGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            complement(5799..8096)
                     /gene="bglX"
                     /locus_tag="KLK57_000253"
     CDS             complement(5799..8096)
                     /gene="bglX"
                     /locus_tag="KLK57_000253"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HBE0890742.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLITALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            8292..10007
                     /gene="dld"
                     /locus_tag="KLK57_000254"
     CDS             8292..10007
                     /gene="dld"
                     /locus_tag="KLK57_000254"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBE0890743.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYIHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            complement(10046..10978)
                     /gene="pbpG"
                     /locus_tag="KLK57_000255"
     CDS             complement(10046..10978)
                     /gene="pbpG"
                     /locus_tag="KLK57_000255"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HBE0890744.1"
                     /translation="MPKFRVSLFSLALMLAVPLAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(11152..11739)
                     /locus_tag="KLK57_000256"
     CDS             complement(11152..11739)
                     /locus_tag="KLK57_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HBE0890745.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            11909..12487
                     /locus_tag="KLK57_000257"
     CDS             11909..12487
                     /locus_tag="KLK57_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0890746.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            complement(12617..13378)
                     /locus_tag="KLK57_000258"
     CDS             complement(12617..13378)
                     /locus_tag="KLK57_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0890747.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLELGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(13431..14867)
                     /gene="mdtQ"
                     /locus_tag="KLK57_000259"
     CDS             complement(13431..14867)
                     /gene="mdtQ"
                     /locus_tag="KLK57_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HBE0890748.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAVATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLNIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIVGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            15091..15174
                     /gene="yohP"
                     /locus_tag="KLK57_000260"
     CDS             15091..15174
                     /gene="yohP"
                     /locus_tag="KLK57_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HBE0890749.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            complement(15547..16494)
                     /gene="dusC"
                     /locus_tag="KLK57_000261"
     CDS             complement(15547..16494)
                     /gene="dusC"
                     /locus_tag="KLK57_000261"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HBE0890750.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            16733..17131
                     /locus_tag="KLK57_000262"
     CDS             16733..17131
                     /locus_tag="KLK57_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HBE0890751.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            17128..17823
                     /locus_tag="KLK57_000263"
     CDS             17128..17823
                     /locus_tag="KLK57_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HBE0890752.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            17953..18837
                     /gene="cdd"
                     /locus_tag="KLK57_000264"
     CDS             17953..18837
                     /gene="cdd"
                     /locus_tag="KLK57_000264"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HBE0890753.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            18987..19706
                     /gene="sanA"
                     /locus_tag="KLK57_000265"
     CDS             18987..19706
                     /gene="sanA"
                     /locus_tag="KLK57_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HBE0890754.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFRREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            19709..19948
                     /locus_tag="KLK57_000266"
     CDS             19709..19948
                     /locus_tag="KLK57_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HBE0890755.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            20142..21380
                     /gene="preT"
                     /locus_tag="KLK57_000267"
                     /pseudo
     CDS             20142..21380
                     /gene="preT"
                     /locus_tag="KLK57_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
     gene            21374..22609
                     /gene="preA"
                     /locus_tag="KLK57_000268"
     CDS             21374..22609
                     /gene="preA"
                     /locus_tag="KLK57_000268"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HBE0890756.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRRLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELRDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(22736..23746)
                     /gene="mglC"
                     /locus_tag="KLK57_000269"
     CDS             complement(22736..23746)
                     /gene="mglC"
                     /locus_tag="KLK57_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HBE0890757.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(23762..25282)
                     /gene="mglA"
                     /locus_tag="KLK57_000270"
     CDS             complement(23762..25282)
                     /gene="mglA"
                     /locus_tag="KLK57_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HBE0890758.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(25343..26341)
                     /gene="mglB"
                     /locus_tag="KLK57_000271"
     CDS             complement(25343..26341)
                     /gene="mglB"
                     /locus_tag="KLK57_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HBE0890759.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(26621..27661)
                     /gene="galS"
                     /locus_tag="KLK57_000272"
                     /pseudo
     CDS             complement(26621..27661)
                     /gene="galS"
                     /locus_tag="KLK57_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
     gene            complement(27803..28960)
                     /locus_tag="KLK57_000273"
     CDS             complement(27803..28960)
                     /locus_tag="KLK57_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HBE0890760.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRT
                     WTPDASAILYEKYWKLHGGVEAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTMLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(28977..29645)
                     /gene="folE"
                     /locus_tag="KLK57_000274"
     CDS             complement(28977..29645)
                     /gene="folE"
                     /locus_tag="KLK57_000274"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HBE0890761.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            29903..30739
                     /gene="yeiG"
                     /locus_tag="KLK57_000275"
     CDS             29903..30739
                     /gene="yeiG"
                     /locus_tag="KLK57_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBE0890762.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSTYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(30771..32762)
                     /gene="cirA"
                     /locus_tag="KLK57_000276"
     CDS             complement(30771..32762)
                     /gene="cirA"
                     /locus_tag="KLK57_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
                     /protein_id="HBE0890763.1"
                     /translation="MFRLNPVVRVGLCLSAISCVWPVLAVDDDGETMVVTASSVEQNL
                     KDAPASISVITQEDLQRKPVQNLKDVLKEVPGVQLTNEGDNRKGVSIRGLDSSYTLIL
                     VDGKRVNSRNAVFRHNDFDLNWIPVDSIERIEVVRGPMSSLYGSDALGGVVNIITKKI
                     GQKWSGTVTVDTTIQEHRDRGDTYNGQFFTSGPLIDGVLGMKAYGSLAKREKDDPQNS
                     TTTDTGETPRIEGFSSRDGNVEFAWTPNQNHDFTAGYGFDRQDRDSDSLDKNRLERQN
                     YSVSHNGRWDYGTSELKYYGEKVENKNPGNSSPITSESNTVDGKYTLPLTAINQFLTV
                     GGEWRHDKLSDAVNLTGGTSSKTSASQYALFVEDEWRIFEPLALTTGVRMDDHETYGE
                     HWSPRAYLVYNATDTVTVKGGWATAFKAPSLLQLSPDWTSNSCRGACKIVGSPDLKPE
                     TSESWELGLYYMGEEGWLEGVESSVTVFRNDVKDRISISRTSDVNAAPGYQNFVGFET
                     GANGRRIPVFSYYNVNKARIQGVETELKIPFNDEWKLSINYTYNDGRDVSNGENKPLS
                     DLPFHTANGTLDWKPLALEDWSFYVSGHYTGQKRADSATAKTPGGYTIWNTGAAWQVT
                     KDVKLRAGVLNLGDKDLSRDDYSYNEDGRRYFMAVDYRF"
     gene            complement(33054..34523)
                     /gene="lysP"
                     /locus_tag="KLK57_000277"
     CDS             complement(33054..34523)
                     /gene="lysP"
                     /locus_tag="KLK57_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HBE0890764.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(34728..35609)
                     /locus_tag="KLK57_000278"
     CDS             complement(34728..35609)
                     /locus_tag="KLK57_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0890765.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            35708..36757
                     /locus_tag="KLK57_000279"
     CDS             35708..36757
                     /locus_tag="KLK57_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HBE0890766.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATESVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            36831..37688
                     /gene="nfo"
                     /locus_tag="KLK57_000280"
     CDS             36831..37688
                     /gene="nfo"
                     /locus_tag="KLK57_000280"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HBE0890767.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAVEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKFRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            37692..38780
                     /locus_tag="KLK57_000281"
     CDS             37692..38780
                     /locus_tag="KLK57_000281"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBE0890768.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(38836..40086)
                     /locus_tag="KLK57_000282"
     CDS             complement(38836..40086)
                     /locus_tag="KLK57_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBE0890769.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(40186..41127)
                     /gene="rihB"
                     /locus_tag="KLK57_000283"
     CDS             complement(40186..41127)
                     /gene="rihB"
                     /locus_tag="KLK57_000283"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HBE0890770.1"
                     /translation="MEKRKIILDCDPGHDDAIAIMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGEMFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            41257..41955
                     /gene="yeiL"
                     /locus_tag="KLK57_000284"
     CDS             41257..41955
                     /gene="yeiL"
                     /locus_tag="KLK57_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HBE0890771.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPLLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(42022..43272)
                     /locus_tag="KLK57_000285"
     CDS             complement(42022..43272)
                     /locus_tag="KLK57_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HBE0890772.1"
                     /translation="MDIMRSVAGMVVLLAIAFVLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSIIEAAANGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFANVSLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTSGTLDVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(43366..44304)
                     /gene="psuG"
                     /locus_tag="KLK57_000286"
     CDS             complement(43366..44304)
                     /gene="psuG"
                     /locus_tag="KLK57_000286"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HBE0890773.1"
                     /translation="MSELTLSPELLQISAEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSPFEVSIRLDSAREIARA
                     MAVKWQTGLNGGLVVANPIPEQFAMPEETINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLVG"
     gene            complement(44292..45233)
                     /locus_tag="KLK57_000287"
     CDS             complement(44292..45233)
                     /locus_tag="KLK57_000287"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HBE0890774.1"
                     /translation="MREKDYIVIIGSANIDVAGYSHHPLNYADSNPGKIKFTPGGVGR
                     NIAHNLALLGKNAWLLSAVGGDFYGQSLLAQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMSISDCISAEFLAQHQEFIRGAKVIVADCNLSEEVLVWVLENSGETP
                     VFIDPVSAWKCVKIRDHLSKIHTLKPNRLEAETLSGIALSGREDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDINGESGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFIDSV
                     RFAQGCSSMALACEYTNNPELSIANVRSLVENTECLN"
     gene            complement(45691..47382)
                     /gene="fruA"
                     /locus_tag="KLK57_000288"
     CDS             complement(45691..47382)
                     /gene="fruA"
                     /locus_tag="KLK57_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HBE0890775.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVAATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILDGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPETQIVEKNA"
     gene            complement(47399..48337)
                     /gene="fruK"
                     /locus_tag="KLK57_000289"
     CDS             complement(47399..48337)
                     /gene="fruK"
                     /locus_tag="KLK57_000289"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HBE0890776.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTLADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(48337..49467)
                     /gene="fruB"
                     /locus_tag="KLK57_000290"
     CDS             complement(48337..49467)
                     /gene="fruB"
                     /locus_tag="KLK57_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HBE0890777.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEVGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            49835..51016
                     /gene="setB"
                     /locus_tag="KLK57_000291"
     CDS             49835..51016
                     /gene="setB"
                     /locus_tag="KLK57_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HBE0890778.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLADVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(51013..51267)
                     /locus_tag="KLK57_000292"
     CDS             complement(51013..51267)
                     /locus_tag="KLK57_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HBE0890779.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            51422..51994
                     /gene="yeiP"
                     /locus_tag="KLK57_000293"
     CDS             51422..51994
                     /gene="yeiP"
                     /locus_tag="KLK57_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HBE0890780.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            52217..53683
                     /locus_tag="KLK57_000294"
     CDS             52217..53683
                     /locus_tag="KLK57_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBE0890781.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKCSPELAGWIKKHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFVHISDCMQDRVFRHAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            53801..54787
                     /gene="yeiR"
                     /locus_tag="KLK57_000295"
     CDS             53801..54787
                     /gene="yeiR"
                     /locus_tag="KLK57_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HBE0890782.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYELWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            54826..55539
                     /gene="lpxT"
                     /locus_tag="KLK57_000296"
     CDS             54826..55539
                     /gene="lpxT"
                     /locus_tag="KLK57_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HBE0890783.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            55951..56517
                     /gene="mepS"
                     /locus_tag="KLK57_000297"
     CDS             55951..56517
                     /gene="mepS"
                     /locus_tag="KLK57_000297"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HBE0890784.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            56698..58254
                     /locus_tag="KLK57_000298"
     CDS             56698..58254
                     /locus_tag="KLK57_000298"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HBE0890785.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALNINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            58336..60150
                     /locus_tag="KLK57_000299"
     CDS             58336..60150
                     /locus_tag="KLK57_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE0890786.1"
                     /translation="MIVRILLLFIALFTFGVQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPSKEDMLSLFSLPIFPEKYWKDHKLSDPLATPPLASGPYRVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNETGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            60151..61245
                     /locus_tag="KLK57_000300"
     CDS             60151..61245
                     /locus_tag="KLK57_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HBE0890787.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            61245..62270
                     /locus_tag="KLK57_000301"
     CDS             61245..62270
                     /locus_tag="KLK57_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0890788.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            62272..63861
                     /gene="yejF"
                     /locus_tag="KLK57_000302"
     CDS             62272..63861
                     /gene="yejF"
                     /locus_tag="KLK57_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HBE0890789.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFAAPQQEYTRQLLALS"
     gene            complement(63865..64209)
                     /locus_tag="KLK57_000303"
     CDS             complement(63865..64209)
                     /locus_tag="KLK57_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890790.1"
                     /translation="MTSLQLSIVHRLPQNFRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(64542..65662)
                     /locus_tag="KLK57_000304"
                     /pseudo
     CDS             complement(64542..65662)
                     /locus_tag="KLK57_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311101.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
     gene            complement(65690..66385)
                     /gene="rsuA"
                     /locus_tag="KLK57_000305"
     CDS             complement(65690..66385)
                     /gene="rsuA"
                     /locus_tag="KLK57_000305"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HBE0890791.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLVSPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            66535..68295
                     /locus_tag="KLK57_000306"
     CDS             66535..68295
                     /locus_tag="KLK57_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HBE0890792.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NIDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRSDEKALFRDCIYELPLRYMIKHSYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIENFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRPPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            68420..68704
                     /gene="rplY"
                     /locus_tag="KLK57_000307"
     CDS             68420..68704
                     /gene="rplY"
                     /locus_tag="KLK57_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HBE0890793.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(68843..69850)
                     /gene="yejK"
                     /locus_tag="KLK57_000308"
     CDS             complement(68843..69850)
                     /gene="yejK"
                     /locus_tag="KLK57_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HBE0890794.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNTKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWEPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            70032..70259
                     /locus_tag="KLK57_000309"
     CDS             70032..70259
                     /locus_tag="KLK57_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HBE0890795.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMATNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            70279..72039
                     /gene="pbgA"
                     /locus_tag="KLK57_000310"
     CDS             70279..72039
                     /gene="pbgA"
                     /locus_tag="KLK57_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HBE0890796.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGHYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            72114..72190
                     /locus_tag="KLK57_000311"
     tRNA            72114..72190
                     /locus_tag="KLK57_000311"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72148..72150,aa:Pro,seq:ggg)
     gene            complement(72293..74293)
                     /gene="yejO"
                     /locus_tag="KLK57_000312"
                     /pseudo
     CDS             complement(72293..74293)
                     /gene="yejO"
                     /locus_tag="KLK57_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter YejO"
BASE COUNT        18239 a        19577 c        18244 g        18233 t
ORIGIN      
        1 caatgttagg tgaaaaaaga ctggtttcat ctgtaacaaa agaggatttg cttttcgtaa
       61 ggagagatct gttgaccggt tatcaaaagc tttctaacgg aaaaatttct tccataaaag
      121 gacgctcagt ggtcacagta aactactata tgacaaccat agctggaatg tttcaatttg
      181 caacagataa tggttataca tcaggaaatc catttaacgg tctgacacca ttaaaaaagt
      241 ccaagataga accagatccc ctcacccgtg acgaatttat tcgttttatt gaggcttgcc
      301 gtcatcaaca aacaaaaaac ctgtggatta tcgctgtata cacgggtatt cgtcacgggg
      361 agctggtatc actggcctgg gaagatatag accttaaagc gaggactata accatccgcc
      421 ggaattatac aaaactaggc gaattcactc caccaaaaac tgatgctggt accggaagaa
      481 cgattcatct agttcaacca gctattgatg ctcttaaaag ccaagcggaa atgaccatgc
      541 ttggaaaaca gcattctgta gaggtaaagc agagggaata tgggagaagt actgtgcata
      601 aatgcacttt tgtttttagt cctcaggtaa taaaacagcg gcagttttcc ggaccgcact
      661 ataaggttga ctccatcagg gagtcatgga caagtatctt aaaacgcgca ggtctgagac
      721 acagaaaatc gtaccaatcc aggcatactt atgcatgctg gtcacttgcc gctggagcta
      781 atcctagttt tatcgcaagc cagatgggcc acacaaacgc acaaatggta ttcaatgttt
      841 acggagcatg gatgaaagac aacaatcacg aacagataga actccttaac aaaagactat
      901 ctgaaagtgt cccatgtatg ccccataaga aagtggggta aaataaaaac ttgtaaaatc
      961 agttagttta cccttaatcc ctgtcacgtt acgcgcgtgg cagaggcgtt acgggttgct
     1021 gaaaccgcaa cggacagacg gcggtcatcg actgttcaac gatgccgata ttgaccggat
     1081 ccgcgagatc aaacgctgga tcgacaacgg cgtgcaggtc agcaaagtta aaatgctgct
     1141 cagtaatgaa aatgttgatg tgcagaacgg ctggcgcgat cagcaagaaa cattactgac
     1201 ctacctgcaa agcggcaatc tgcatagcct gcgaacgtgg atcaaagagc gcggtcagga
     1261 ttaccccgcc cagacactca ccacacatct gtttattcct ctgcgccgac ggcttcagtg
     1321 ccaacaaccg actctccagg cgctgctggc gatcctcgac ggcgtactga tcaactacat
     1381 cgccatttgt ctggcttcgg cacgtaaaaa acagggtaaa gatgcgctgg tggttggctg
     1441 gaatattcag gataccaccc gtctgtggct ggagggctgg attgccagtc aacaaggatg
     1501 gcgcattgat gtcctcgccc actcgctcaa tcaactacgc cctgaactat tcgaaggccg
     1561 tacattgctg gtgtggtgcg gtgaaaatcg aacctccgcc caacagcagc aactcaccag
     1621 ttggcaagaa caaggccatg atattttccc actcggcatt taatgattcg ttaacaaatg
     1681 cgctttactg tacaatcctt tcgttaacat aaggagtgca atatgcgcat agctaaaatt
     1741 ggggtcatcg ccctgttcct gtttatggcg ttaggcggaa ttggtggcgt catgctcgca
     1801 ggttatacct ttattttgcg tgctggctaa gcgcctgcac cagcctttca aacaggcggt
     1861 ctgcgatgat cgccgccagt gccaccagta acgccccctg gatcacatac gcggtattaa
     1921 atccgctaag cccgatgatg atgggcgtac ccagcgtgct ggcccctacc gttgaggcga
     1981 tcgtcgccgt accaatgttg ataatcaccg aagttcgcac gcccgccaga atcaccggag
     2041 ccgccagcgg tagctcgacc ttacgcagtc gctgaccacg actcattccc atacctttcg
     2101 caacttctgt cacgctggca tcaatcgctc ccagcccggc aagtgtcgcc tgcaggacgg
     2161 gcagcacacc gtaaaggatc aaggcgataa tcgctggttg cagaccaaag ccgatcaccg
     2221 gaacggcaat cgccagcact gcgacaggcg gaaaagtctg tccaacggcg gcaatagttt
     2281 ccaccagtgg gcgaaattcc gcgccccacg ggcgagtgac agcaattccg gcaccagtgc
     2341 caatgatcac cgcaaacaaa ctcgaaattc ccaccagcca gaaatgagcc agtgccagag
     2401 ctgcaaaact ttcttgctga taaacgggtc gtggcagttg tgggaacaag gcagcaaaca
     2461 gcggctggct gtaaggcagc cagaaaatca gtgccacaaa caaagcaatg agccagaaca
     2521 gcggatcgcg caacatcttc atacgcttac gcctcctcca gcagatcctg aaaatgcagc
     2581 gtgccgcaag gctggccctg cgtgttcacc accggcagca cctcgcatcc ccgcgcgaca
     2641 aacagggaga gcgcatcgcg tagcgtcatc tcttctgcca gtgcctcacc ttctgcccgt
     2701 tcttcgcgac gcacgtaatc cgccacacta cgtaacgaaa gcaggcgcac acccagttcg
     2761 ctgcgcccaa aaaactggcg gacaaaatcg tttgccggac gagtcagcat cgtcagcgga
     2821 ttgccctgct gcaccacttc accgtgatcc atcaatacca gatgttctgc cagccgtagc
     2881 gcctcatcaa tatcatgagt gaccagcaca atggtacgcc ccagcaaacg gtgaatgcgc
     2941 gtcatctctt gttgcaacgc gccgcgcgtt accgggtcca gtgcgccaaa aggttcatcc
     3001 atcagtaaga cttgcggatc ggcagccagt gcgcgcgcca ctcccacacg ttgctgctga
     3061 ccaccggaaa gctgatgcgg ataacgctca cgtaaatttg actccagccc cagtagcgcc
     3121 attaattcgt cgatacgatc gtcaatcctc gcccgcgacc atttttgtaa ttgcggcacg
     3181 gtagcgatgt tttgcgccac gctccagtgg gggaacaggc caatagattg aatggcatag
     3241 cccatccggc ggcgcaactc cagtactggc agcgagcgaa tttcttctcc ggcaaagcgg
     3301 atcactccgc tgtcatgctc caccaggcgg ttaatcattt tcagggtggt ggatttgccg
     3361 gagctagatg tgccaatcag caccgaaaaa ctcccttcct gaaaattgag attgagatcg
     3421 ttaacggctt tttgtgcgcc gaacagtttg ctgacatggc caaattcaat cattacgttt
     3481 caccttcagc agtgcgataa gcaaatcgaa cagcgcgtcg gtaagcaccg ccagaacaat
     3541 taccgggatc acccccagca acactaaatc aatggcgctg cttagcagcc cctggaaaac
     3601 cagcgcacca aaaccgcctg cgccgattaa cgccgcaatc accgccatac ctacagtttg
     3661 caccatcacc acccgcaggc tgcgcagaaa taccggtaac gccagtggta actgaacatg
     3721 caggaatcgc tgcgccccgc tcatgcccat cgctctggcg ctctccagca catcgcgcgg
     3781 gatctggttc aaaccgacta ccacgccgcg caccagcggc agcaaggcat agagcaccag
     3841 cgcaatcagt gcgggtgtca ttccggttcc tgctatgccg agcgtcccca gccacggaaa
     3901 ggccgtaacc agcgcggcaa gcggcgcaat caacaggcca aagagcgcca ccgaaggcac
     3961 ggtctgaatg acattgagca gagaaaaaat tgccccctgc cgagcagtgg aaaagtagca
     4021 ccagatgccc aacggcacac caatcactaa cgcaggcagc accgcaccaa acagcaacgt
     4081 cagatgttgt gccagcgcgt cgtcaaacac atcctgacgg ttggcgtatt ctttcattag
     4141 tgagagatcg ttaagcgtgc cggagtacag caaccacagc ggaataatgg caatctgcat
     4201 atgcaacaac cagcgccaca gcggatgcgt ggagattcgg cggatggcat cactacaggc
     4261 cagcaatgcc agcgccgcgg ccagccagaa accactgccg aggctggtac gcgccagcgc
     4321 actgccattt tgcgccagtt gggtcgccgc ctttcctgct ccccacacca gcaatacgaa
     4381 gacgaattgc gccagaatga gtgcaaaaat gctgcctttt ttagcgggaa taaaacaggc
     4441 tgtcagccag gcgcaaccaa cgcccaccag catccagatc gtttgcggcc acagttgcca
     4501 gagatgacgc ccttcacccg agactaaacg attaggcgcg taactgataa acggcagcgc
     4561 cgctgcgatt gccgtcagca acagcagcag cgccagaaca ggattaatac gtagataagt
     4621 cacgggaaat tacttcgtcc accctttttg tttcaggtag tcggcagcca cttttttggc
     4681 atccagtcct tccactgcaa tgctggcatt cagttgctgc aatgtttttg catcgaggct
     4741 ggcgaagact ggctgtagcc actgtgccat ttgcggatac tctttcagca ccgactcacg
     4801 caccactggt gcaggcgcgt agataggttg cacgcctttc ggatcgctta aggtttgcag
     4861 ccccagcgcc gcgaccggac cgtcagtgcc gtaagccatt gcggcattaa cgccagaagt
     4921 ttgctgggca gcggctttaa tcgttaccgc cgtgtcgccg ccagccagtg acagtaactg
     4981 atcctgatcg agtttaaagt cgtaggcttt ttcaaacgcg ggtaacgcat cggcgcgttc
     5041 gataaactct gccgaggctg ccagtttgaa ggtgccgccc tctttcagat aacgactcag
     5101 gtcggcaagc gaagtgagtt tgtttttctc tgccacatcc tgacgcacgg cgatggtcca
     5161 ggtgttattc gctggtgcgg gcgtcagcca gattaacttg ttttgctctg catcgagttt
     5221 tttgactttc tcgtaacctt gctgcgcgtt tttccatgcc gcatcgtttt catctttaaa
     5281 gaagaaagcg ccattaccgg tatattccgg atagatatcc agttcaccgg aagtaatcgc
     5341 cccgcgcacc acaggagtcg ttccgagttg cactttattc accgtcggta cgccgtggct
     5401 ttccagcacc tgcaagatga tgttgccgag tagcgcgcct tcggtatcga tttttgaacc
     5461 gactttaacg ggggaagccg cctgtagcgg caggctcacg gctgccaaca taaccagtga
     5521 acctgcccag acctttgaga gtggcatgat gctttcctca ttcttttact gttgttttca
     5581 gcgaattaag agaaaagcat agttgataat gacagagtta gcctgagcgg aaggattcag
     5641 ttgcagaatc agataaatac ttaaagggca tattttgccc ggcgtttctt gccggatgcg
     5701 gcgtaaacgc cttatccggc ctacaaaggt cgaaaattca gcaagttaag atatgaacat
     5761 caggcaaagg gcgcaaacgt cgcgccctta ctaaagcatt acagcaactc aaactcgcct
     5821 ttcttaacgc gtgcggaatc agtgccgata aagacattga acttgccagg ttcggcgtca
     5881 tatttcatct gttgattcca gaacttcagc gcctcgatat caatcgggaa gctgacagtc
     5941 tgagtttcgc ccggtttcag ggtgattttc tcaaagcctt tcagctgctt cactggacgg
     6001 ctcatggaag ccgtcacatc ctgcaagtac atctgtacta ccgtggcccc ttcgcgttta
     6061 ccggtgttcg tcacctgcac gctggcggtc actttgccgt cacgcttcat ggtcggcgca
     6121 gaaagtttca catcagagac ggtgaaagtg gtatagctca gaccatagcc gaacggataa
     6181 agcgccccgt tagcttcatc aaaataacgc gaagtgtatt tgttcggctt gtcggcatta
     6241 tacggacgac cggtattcag atggctgtag tacaccggga tctgcccgac agaacgcggg
     6301 aaggacattg gcagcttgcc ggacgggttg taatcgccaa acaatacatc ggcaattgca
     6361 ttaccgcctt cagtccccgc aaaccaggtt tccagaatcg catcagcctg ctgatcttct
     6421 ttcaccagcg ccagcggacg cccgttcatc agcaccagca ccagcggttt accggtggct
     6481 ttcagcgccg taatcaagtc acgttggctt tgcggaatag tgatatcggt acggctggag
     6541 gcctcgtgcg ccatcccctg tgcttcaccg accacagcca ccaccacatc agattgtttc
     6601 gccgtctgca ccgcttcatc aatcatctct tgcggcgagc gcggatcgac tttgaccgct
     6661 tcttcatact gattcaggaa atcgataatg cctttgtcac tggtaacgtt cgcccctttg
     6721 gcatacagca ctttaccgtt ttcaccgacg gcatttttaa tcccggtcag tacggtcacg
     6781 gattgatcgg caacaccggc tgcggaccag ctgcccatca cgtcacgttt actgtccgcc
     6841 agtggcccaa ccaccgcaat ggtggccgat tttttcagcg gtaacgtttc gagacggttt
     6901 ttcagcaaca ccaggctttc gcgcgccact tcacgcgctt ctttacggtg caggcggctt
     6961 tcggcattgg tatccaccgg gtcagactct ttcggcccca aatggctgta tgggtcgtta
     7021 aacaatccca tatcatattt aacgttcagt acatggcggg cagcatcgtc cagctcttcc
     7081 atcgtcactt tgccggattt gatcaaccca ggcagatact tcgagtaata ctcgtcgctc
     7141 atactcatgt tgattccgga tttcagcgcc acgcgcaccg catcttccgg gtctgccgcc
     7201 gtgccatgtt taatcagctc tttgattgca ccgtgatcgg aaacggtgat gcctttaaag
     7261 ccccactggt cgcgcagaac atctttcagc agccaggaat cggaggtggc tggcgtgccg
     7321 ttcagcgagt tcagcgccac catcaccgcg ccgctgcctg cgtccagccc cgctttgtac
     7381 ggcggcatat aatcattaaa caggcgctgc ggactcatat cgacggtgtt gtactcttta
     7441 ccgccttcta ccgcgccgta tgcggcaaag tgtttgacgc tggtcatcac cgagtagcga
     7501 tctgccgggc ttttaccctg catcgcttcc accatggttt tacccattgt tgaggtgaga
     7561 tacgtatctt cgccaaaacc ttcggaagca cgtccccagc gcggatcgcg cgagacatcg
     7621 accatcggtg cccaggtcat attcaggcca tcatctgccg cttcataagc agagacacgt
     7681 ccgaccgttt tcactgcatc gaggttaaaa gacgaggcca gaccgaggct aatcgggaac
     7741 accgtgcgct gaccgtgcag cacgtcgtaa gcaaagaaaa gaggaatttt caggcggctt
     7801 aattccatca cctgatcctg catggcgcgg atatcctgac gggttacggt gttgaaaatc
     7861 gccccaacct gaccgtcttt gatcatctcg cggatcgcct ctttcgggtt atccggaccg
     7921 acgctgatta agcgcagctg accaattttc tcatcaactg tcattttctt aagcagttcg
     7981 gtgacgaacg catcccgcgc ttcgggcgtt aatggatggt tgccgaataa atcatccgcc
     8041 agtgctggct gcagggccag actcaccgcg attcctactg aacatagcca tttcatatgg
     8101 atttttcttc ctctgttgcc cgacgaggca gcgaaaatgg tgcaaaaacc gtagtttgcc
     8161 ataagcatga tggagagaga aaaagaatgc tcagtttatt gtctgaattt tcaaaatatt
     8221 cactcgctga attgtcatac aaggcgctat tctagtttgt gatatttttt cgccaccaca
     8281 aggagtggaa aatgtcttcc atgacaacaa ctgataataa agcctttttg aatgaacttg
     8341 cccgtctggt cggtcattca cacctgctca ccgatcccgc aaaaacggcc cgctatcgca
     8401 agggcttccg ttctggtcag ggcgacgcgc tggctgtcgt tttccctggc tcactactgg
     8461 aattgtggcg ggtgctgaaa gcctgtgtca ccgccgacaa aattattctg atgcaggctg
     8521 ccaatacagg cctgaccgaa ggatcgacgc caaacggtaa cgactatgat cgcgatatcg
     8581 tgatcatcag caccctgcgt ctcgacaagc tgcacgttct tggcaagggc gaacaggtgc
     8641 tggcctatcc gggcaccacg ctctattcgc tggaaaaagc cctcaaaccg ctgggacgcg
     8701 aaccgcactc agtgattgga tcatcgtgta taggcgcatc ggtcatcggc ggtatttgta
     8761 acaactccgg cggctcgctg gtgcaacgtg gcccggcgta taccgaaatg tcgttattcg
     8821 cgcgtataaa tgaagacggc aaactgacgc tggtgaacca tctggggatt gatctgggcg
     8881 aaacgccgga gcagatcctt agcaagctgg atgatgatcg catcaaagat gacgatgtgc
     8941 gtcacgatgg tcgtcacgcc cacgattatg actatgtcca ccgcgttcgt gatattgaag
     9001 ccgacacgcc cgcacgttat aacgccgatc ctgatcggtt atttgaatct tctggttgcg
     9061 ccgggaagct ggcggtcttt gctgtacgtc tagatacctt cgaagcggaa aaaaatcagc
     9121 aggtgtttta tatcggcacc aaccagccgg aagtgctgac cgaaatccgc cgtcatattc
     9181 tggctaactt cgaaaatctg ccggttgccg gggaatatat acaccgggat atctacgata
     9241 ttgcggaaaa atacggcaaa gacaccttcc tgatgattga taagttaggc accgacaaga
     9301 tgccgttctt ctttaatctc aagggacgca ccgatgcgat gctggagaaa gtgaaattct
     9361 tccgtccgca ttttactgac cgtgcgatgc aaaaattcgg tcacctgttc cccagccatt
     9421 taccgccgcg catgaaaaac tggcgcgata aatacgagca tcatctgctg ttaaaaatgg
     9481 cgggcgatgg cgtgggcgaa gccaaatcgt ggctggtgga ttatttcaaa caggccgaag
     9541 gcgatttctt tgtctgtacg ccggaggaag gcagcaaagc gtttttacac cgtttcgccg
     9601 ctgcgggcgc agcaattcgt tatcaggcgg tgcattccga tgaagtcgaa gacattctgg
     9661 cgttggatat cgctctgcgg cgtaacgaca ccgagtggta tgagcattta ccgccggaga
     9721 tcgacagcca gctggtgcac aagctctatt acggccattt tatgtgctat gtcttccatc
     9781 aggattacat agtgaaaaaa ggcgtggatg tgcatgcgtt gaaagaacag atgctggaac
     9841 tgctacagca gcgtggcgcg caataccctg ccgagcataa cgtcggtcat ttgtataaag
     9901 caccggagac gttgcagaag ttttatcgcg agaacgatcc gaccaacagt atgaatccgg
     9961 ggatcggtaa aaccagtaag cggaaaaact ggcaggaagt ggagtaaaaa ttacggatgg
    10021 catagtatcg ccatccgtca ttcacttaat cgttctgtgc cgtctgcccc gccgccgcca
    10081 tttgggcggc tttttgtttt ttatagctca acgctgctgc tggaacaggc atcactttac
    10141 cagtttcaat ccaggtacgc aggcggctgg catcggcaaa atgggtatat ttgccaaacg
    10201 catccatcac taccagcgct accggtttat tattgataac cgtacgcatc accagacaat
    10261 ggcccgccgc attggtaaaa ccggttttgg ttaactgaat attccagtta tcgcgataca
    10321 ccagatggtt agtattgcgg aacggcagcg tatacgtcgg gttagagaag gttgccatat
    10381 cttcccgggt agtacttaac tgcccgatca acggatattg tttgctggca atgagcagtt
    10441 tggttaagtc acgggcagtt gaaacgttat gcaccgacaa tccggtaggt tcaacaaagc
    10501 gcgtgttgtt cattccgagc gatttcgctt tcgcattcat tgccttaata aaggctttgt
    10561 aaccaccggg ataatggtgt gcaaggcttg ccgccgcgcg gttttctgaa gacatcagcg
    10621 ccagcaacag catatcttta cggctgattt cgctattcag tcgtacgcgc gaatagaccc
    10681 ctttcatctc cggcgtctgg ctgatatcca cttttagttt ttcatccagc ggcagtcgtg
    10741 catccagcac aaccatcgcg gtcattaatt tgctgataga cgcaatcgga cgcaccagat
    10801 ccgggtggtt cgagtagatc actttgttgg tattcagatc aacaatcatc gcgctaccgg
    10861 aggcaatttc aggttgtgaa gcggtggtag cggctgccgt tttcgcaacg gcctgcggtg
    10921 ccaaaggcac agccagcatc agggccaggc taaataaaga aactcgaaat ttcggcatga
    10981 tgagcattca gatagtggtt cacgcgcaca ggttgcgcac cgccggagta aggatttact
    11041 gaggctagcg acgccatcat aacgagcaaa aagtgcgatc gtcaaaggag aatcgtgagg
    11101 aaatgctgca ttgctgacat ttacgccagc aatgcaacgt caaagaactt tctagaacaa
    11161 acgataaccg tagccccata gtataacggt tagggcgagc agcacttcca gtaccagcac
    11221 gccaatcgcc agcgtcgaac tagagaagct aaggccttcc tctttgttga tattcaggaa
    11281 gctcggaata ccaaggtaaa gcaggtagcc ggtgtaaaac agcgccaccg tgccgaccag
    11341 cgcacacaac cagaccagtg gataaagcgc caccagaccg cttaaaaaca gcggagttgc
    11401 aacgtagccc gcgaagacca tgcagtgcgc aagtgacgga cgctgcggat aattacgcgc
    11461 catccaccag atgacccgcc ccattaccgc aaccccagcc agcataacgc cataaaacag
    11521 gacagccagc gccagtccgg taaaccagga taacttcagg atagtgccat cgccaaaatt
    11581 ccagccaatc tgtgtagtgc caatgaaggc gcaaatcacc ggaatcgccg ccatcagcaa
    11641 aacgtggtgg gtgtaatggt gagaaatcgt ttcgttttcg cgattaatca cctgcatttc
    11701 acgatcggga tgggaaaaca gtccccagac atggctcata ccgccccctt gttgtgagtt
    11761 catgaacctg acagttcaag tataagtcag cttgtgatta ttttttgttc gctcatgtaa
    11821 tttgcaccgt ctgatgtacg tcctttttca gcgtgaatga tttccagggt gtatgcttac
    11881 aggcaaccaa agggagacag actggcctat ggatctcaat acactcatct cacaatatgg
    11941 ttatgccgcg ctggtgatcg gtagcctggc ggaaggtgaa accgtgactt tgctgggagg
    12001 cgttgcggca catcaggggc tattaaagtt cccgctggtg gtactttctg tggcgcttgg
    12061 cggcatgatt ggcgaccagg tgctttatct gtgcgggcgg cggtttggcg gcaagctgtt
    12121 acgccgtttc tcgaaacatc aggataaaat tgagcgggcg cagaaactta tccaacgcca
    12181 tccgtatctg tttgtcattg gtacgcgctt tatgtatggc tttcgggtga ttggcccgac
    12241 gctgattggt gccagccagc tgccgccgaa aatctttctg ccgctgaata ttctcggcgc
    12301 atttgcctgg gcgttgattt ttaccactat tggttacgct ggtggtcagg tgattgcgcc
    12361 gtggttgcac aatctcgacc agcatttgaa gcactgggtc tggttgattc tggtcgtggt
    12421 tctggtggtg ggcgtgcgct ggtggctgaa acggcgcggg aagaaaaagc cggatcatca
    12481 ggcgtaaaac cattgccctg ggtaaggcgt tcgcgccgca tccgacatcc ggtgcaccat
    12541 gcctgatgcg acgcttgtgc gtcttatcat gcctaccaaa attttaccca ttgaaataac
    12601 cacgcacctc actgcgctat tctgggttga actgcggatt cgccaacata aagccgccat
    12661 ccactatcaa cgactgcccg gtggtgtaat ttgcgccctc tgagcacagc cacgctacca
    12721 ggctggcaat ctcatgcgtt gcgccaaaac gccgcagagg aatcgaaggc tccgcgtcgg
    12781 gcttcacgtc gctgtcatcc atgccattca ttggcgtggc gatcgcccca ggcgcgactg
    12841 cgttcaccaa aatcttatgc ctgaccagct ccagcgccat cgttttggtt aacccgccga
    12901 gcgcatgttt agcggctgtg taagcgctgg catccggcag cggcgtatgt tcatgtaccg
    12961 acgtaatgtt gatgatacga ccgccctgcc cttgtttcac catctggcgg gccgcaattt
    13021 gtgagcataa aaatgcacca tcgacatcaa cggtaaaaat cttgcgccac tcatcgaaag
    13081 ccatatcaag aaacggcgct ttggtcattg cgcccgcatt attcaccagc acatcaatac
    13141 gccccagccg ttgaatgagt ttctccagcg cctgcgcacc ttctggcagt ttaccgagct
    13201 ccagctgcac gatctccgca cgtacgccgt ggctaactac ctcacgcgcg gtatcttttg
    13261 ccccttcttc atctgagtgc caggtaatgc caatatcaaa cccctgctgc gccagtaata
    13321 acgcgcactc tttgccgatc cccgaatcgg aggcggtaat aatcgcaacc tgtgccatca
    13381 agttctccac ttaacgctga ataaacgtta agtatagaag gcgcatatca tcagcgtttg
    13441 taacccccgc ccaacgcacc agtgagttga atagaagcat ccagccactg cccttgcaat
    13501 aacaggccat tggcccgctc acgcagcgcg gggattctgg cttcgctgac gcgggaacca
    13561 acaatgatgc ccgcgttaaa gcgcgcctgc gcaagaccta ccacacgcaa ggcatcgcgc
    13621 tcaatttgcg cctgatgctg gtttttctcc gcaagtgtct gaacctgact ggctgcccgc
    13681 gccacgtcat tcaccgcttc aaccaccgct ttgttgtagc tggcgataga caagttgctt
    13741 tcggcttttg cgatattgag attggcgtta agacgaccac tatcgaaaat gggtagcgtc
    13801 aggcctgccg taacgcccat ttgctgcgcg gaatgacgga acagatcgct taagtgcaac
    13861 gcatcctgtt gcaggaaggc catcaggttg atgtcaggat aaaatgccgc ttttgccgca
    13921 tcaatggtgc ttagcgatga ctcaacgtac cagtgcgccg cctgcaaatc tgcccgccgg
    13981 gccagtaagg agtaccccag ttcatcagga agctggcttg ccactttcgg caacgcgacc
    14041 ggtttaagct tcaatgactt tgtctggtta tttgtaagtg cgcttaaccg tgcctcaata
    14101 attttcattt tccccgcgac atcgttgagc tgctgccggg ttttgctggc attaatatcg
    14161 gtttccacac cttcaactga agaagtaatc ccgttctgat atagctggcg atcggtcgcg
    14221 ataatggtgt tctgctcttt ttctatttgc tgcaagaccg tgtttaacgc cgcctgggtt
    14281 tgccactccc agtacaggcg ggctacgctg ccagccagca attggcgggt ttgctcgcgt
    14341 tccgccgccc gtgctttaac cgtacccagg cgggcagtaa cctccgcccg attctttccc
    14401 cagatatcga gatgccagcc cgccgttaag ccaaaagtac cgttggtgta ccacgggccg
    14461 gtcgtacctg cggccggatc gttcagagca aacggcccca ttaagccttc tgccgacatt
    14521 ttttgccgct ccatatccgc cgaaaagtcg atctgcggac catcctgagt ggcaactgcc
    14581 ttcgcctggg cttcagctag ctgaatgcgc tgttcagcca cctgcatatc cggtgcgttc
    14641 tgtagtgcat tgttaattaa tgaagtgagc tgattatcgt gatactccag ccaccattgg
    14701 ctgtctggcc aaccattttt cagcgccgtg ggtaatgcgg tgtcaacttg tgcagcgggc
    14761 gtttgctggc ttaacgcctg gcgggtttca tgcataggcg cacacccggc cagcatcagt
    14821 aacagcggaa aacaggcgat ggctggataa aaggaatcac gattcatggg ggaataatca
    14881 ggtaagaaac ggtgcgcgga gattaccgtg tgttgcgata tattttttag tttcgcgtgg
    14941 caatacatca gtagcaataa aacgacatac ccagaaaaat atagactaag taaatgatat
    15001 cttccgattt atcttaatcg tttatggata acggcaaagg gcttcgtttt ttcctatact
    15061 tattcagcac tcacaaataa aggaacgcca atgaaaatta tactctgggc tgtattgatt
    15121 attttcctga ttgggctact ggtggtgact ggcgtattta agatgatatt ttaaaattaa
    15181 ttaatgtcat caggtccgaa aataacgaga atatttcagt ctctcatcct gttgcgctcc
    15241 tgtcatgtgc attgcttcat ataatcactg gcgcaaggag cgcgcagggg gcggccaatc
    15301 gccgccgccc cctgcacccc cgggctctgg cgaacaaaat cgccgctgcg cggtgccctc
    15361 ggcttatccc ttacggctac cgggtcgggc gcgaggtaac atccctgtaa aacgcgccct
    15421 cagcccacct ccatgtgggc tgccccggcc ttcagggaac gcctcggcaa ttttgacgcc
    15481 accaaacaac cgtgcggcct attgataaag agctaacaca ttgtcaaaaa acatcactat
    15541 ggttttttag agtttctcga tatcaattgc ctgaatagcc cttgcaatat caggggaatt
    15601 attcaacacc cgaacatgct gaaataattc cgttgcttca tcgtattctt tacgcaaata
    15661 actcaaccac tgtttaatcc gcgcaacgtg atataacccg gtatcgccct gcttttccag
    15721 acgggtatat ttttgcagca aagcaaccac ctccggccac ggcattcgcg gttcgttata
    15781 ttttactacc cggctcaggt tgggaatatt gagcgccccg cgaccaatca tcactgcgtc
    15841 gcagccgctg atcgcaatgc attgttgcgc gctctgccag tcccagattt cgccgttggc
    15901 aatcaccgga atattcagcc gctggcgaat ctcgccaatc gcctgccagt caatatgctc
    15961 cgcgcggtaa ccctgctctt ttgtccgtcc atgcaccacc agctccgtag caccagcctg
    16021 ttgaaccgca tcggcgattt caaatttctt ctcaccgctg tcccagccca gacgcacttt
    16081 cacgctgacg ggcaaatgcg ccggtacagc ttcacgcatc gcttttgcac cctggtagat
    16141 gagttcagga tctttgagta acgtcgcccc gccgccgctg ccgttaaccg ttttcgacgg
    16201 gcagccgcaa ttgagatcca cgccccagga acctaattcc accgcacggg cggcgttctc
    16261 tgccagccat tgtgggaact gacctaacaa ctgaacgcgc accagcgtac cggatggtgt
    16321 ccggctggcg ttttgtagct cagggcaaat gcgatgaaag acttttaccg gcagcagttg
    16381 atccaccacg cggacaaact cggtgatgca cagatcgtag tcgttaactt cggtcagcaa
    16441 ttctcgcacc agagagtcaa gcacaccctc catcggagcc agtaacacac gcatatcttc
    16501 acccggaaaa aaatgaggcg ctatgttagc gcctatggtc aacggattaa aggtctgcaa
    16561 agagggattg ctgcaaactg taatccctct ctatgcttga tgtatgcctg agttcgttgc
    16621 gcattgcgta atgccgggga gcgaaaaaat aagcgctgat ttcttaatgt gatcggtagc
    16681 acgttttacg aattaattgt atgatgaatc catctcatct ggggtgttga ttatgagtaa
    16741 gacactgaac attatctggc aatatttacg cgctttcgtc ctgatttatg cctgcctgta
    16801 tgcaggcatt ttcattgctt ccctgctacc ggtaaccatt ccgggcagca tcatcgggat
    16861 gctgatcctg tttgtcctgc tggccttgca aattcttccg gcaaaatggg tcaatccggg
    16921 gtgctacgta ctgattcgct atatggcgct attgtttgtg ccgattggcg taggcgtcat
    16981 gcaatatttt gatttgctcc gcgcacagtt tggcccggta gtggtttcct gtgcagtcag
    17041 tacgctggtg gtttttctgg tggtgagctg gagttcacaa ctggtacacg gtgaacgtaa
    17101 agtcgtaggt cagaaaggat cagaagaatg atggcgaata tctggtggtc attaccgctg
    17161 acgttgattg tcttttttgc cgcccgcaaa ctggcggcac ggtataagtt tcccctgctt
    17221 aacccgttgc tggtagcaat ggtggtgatc attccttttt taatgctgac cggcatctct
    17281 tacgacagct actttaaagg tagcgaagtg ctaaacgatc tgctgcaacc ggcggtcgtc
    17341 gcgctggcct atcctttata tgagcagcta caccagatcc gcgcgcgctg gaaatcgatc
    17401 atcaccatct gttttatcgg cagcgtggtt gcgatggtga cggggacttc cgtggcattg
    17461 ttgatgggcg cttcaccgga aatcgccgcg tcaatcctgc cgaaatcagt caccacgcct
    17521 attgcaatgg cggttggcgg cagtattggc ggtattccgg caatcagcgc agtttgcgtg
    17581 attttcgtcg gcatcctcgg cgcggtattt ggccatacat tgcttaatgc gatgcgtatt
    17641 cgtaccaaag ctgcgcgcgg tctggcaatg gggacagcct cgcacgccct cggtacggcg
    17701 cgctgcgccg agctggatta tcaggaaggt gcatttagtt cgctggcgct ggtgttatgc
    17761 gggataatta cttcgctgat cgcaccgttc cttttcccga ttattctggc agtaatgggc
    17821 taaaatttgc gatgcgtcgc gcatttttga tgtaagtttc acgcgttgca taattaatga
    17881 gattcagatc acatataaag ccataacggg ttcgtaaact gttatcccat tacatgatta
    17941 tgaggcaacg ccatgcatcc acgttttcaa accgcttttg cccaacttgc ggataacttg
    18001 caatctgcac tggaacctat tctggcagac aagtacttcc ccgctttgtt gaccggggag
    18061 caagtctcat cgctgaagag cgcaacgggg ctggacgaag acgcgctggc attcgcacta
    18121 cttccactgg cggcggcctg tgcgcgtacg ccattgtcga attttaatgt tggcgcaatt
    18181 gcgcgcggtg tgagcggaac ctggtatttc ggtgccaata tggaatttat tggtgcaaca
    18241 atgcagcaaa ccgttcatgc cgaacaaagc gcgatcagcc acgcctggtt gagtggtgaa
    18301 aaagcgcttg cagccatcac cgttaactat acgccttgtg gtcactgccg tcagtttatg
    18361 aatgaactga acagcggtct ggatctgcgt attcatctgc cgggccgcga ggcacacgcg
    18421 ctgcgtgact atctgccgga tgcctttggg ccgaaagatc tggagattaa aacgctgctg
    18481 atggacgaac aggatcacgg ctatgcgctg acgggtgatg cgctttctca ggcagcgatt
    18541 gcggcggcaa accgttcgca catgccttac agtaagtcgc caagcggtgt cgcgctggaa
    18601 tgtaaagacg gtcgtatttt cagcggcagc tacgctgaaa acgccgcatt caacccgact
    18661 ctgccaccgt tgcagggggc gttaattctg ttgaatctca agggttatga ttacccggat
    18721 atccagcgcg cggttctggc agaaaaagcc gatgcgccgt tgattcagtg ggatgccacc
    18781 tccgcaacgc tgaaagctct cggctgtcac agtatcgacc gcgtgcttct cgcttaagcc
    18841 tggtgccgga tgcggcgtga acgccttgtc cggcttgcca gccctctcct ggtgtcgaaa
    18901 ttcccggcaa acagtttgcc gtttcttgcg caaaaccagc gggtaaagta gcctgatgga
    18961 aattttcctt agatcgagtc tcctgcatgt taaagcgcgt gttcctcagc ctgttagtcc
    19021 tgatcggctt gctgctgttg actgtgctcg gcctcgatcg ctggatgagc tggaaaaccg
    19081 cgccttatat ctacgacgaa ttgcaggatc tcccctaccg ccaggtcggc gtggtgctcg
    19141 gcacagcaaa atattatcgt actggcgtaa ttaatcagta ttatcgctac cgcattcaag
    19201 gagcgattaa tgcctataac agcggtaagg taaattatct attactgagc ggcgataacg
    19261 cattgcaaag ttataatgag ccgatgacca tgcgcaaaga tttaatcgct gctggtgtcg
    19321 acccatcaga tattgttctc gattacgcag gctttcgtac gctggattcc atcgtgcgta
    19381 cacgcaaagt gttcgatact aatgatttca ttattatcac ccaacgtttc cactgtgagc
    19441 gggcattatt tattgcgctg catatgggga ttcaggctca gtgttatgcc gtaccgtcac
    19501 cgaaagatat gctgtcagta cgtattcgcg aatttgccgc ccgtttcggt gcgctggctg
    19561 acctttatat ttttagacgt gaaccgcgtt ttttagggcc gctggtccct attccggcta
    19621 tgcaccaggt gccggaagat gcgcaggggt atcccgccgt cacacccgaa cagttacttg
    19681 aattacaaaa gaaacaagga aagtagttat ggatgtacag cagttttttg tcgttgccgt
    19741 ttttttcctt atcccgatat tttgtttccg cgaagcatgg aaaggctggc gcgcaggcgc
    19801 gattgataaa cgggttaaaa atgcaccgga accggtgtat gtctggcgag caaaaaatcc
    19861 cggactcttt ttcgcttata tggtggcata tatcggcttc ggaattttat ctatcggcat
    19921 gattgtttat cttattttct atcgttaata ttttctcttt tctgttaatt attgagaata
    19981 attattactt cactcaataa gctgcggata taacattctg aaccgcagct atttgtgaat
    20041 cctttcacag tttaaatttc cccacacgct tagccttaat atcagcacgc aatatttatt
    20101 tagcgttcgc cttaattact catagcatta aggaagatca catgccgcaa caaaattatc
    20161 tggatgaact cactccggct tttacgcctt tactggcgat taaagaagcc tctcgctgtt
    20221 tattatgtca tgacgctccc tgtagtcagg cttgcccggc acagaccgat ccggggaaat
    20281 ttattcgctc aatctacttt cgtaatttta aaggcgctgc cgagacaatt cgcgaaaata
    20341 atgccctcgg tgccgtttgt gccagagtgt gcccgacgga gaaattatgc caaagcggtt
    20401 gtacccgtgc cggtgttgat gctcccattg atatcggccg cttacagcgt tttgttactg
    20461 attttgaaca acaaaccggg atggaaattt atcagcccgg tactaaaacg ctcggcaaag
    20521 tcgcgattat tggcgcaggt cctgccggat tgcaggccag tgtgacactg acaaaccagg
    20581 gttatgacgt cacgatctat gagaaagaag cacaacccgg tggctgattg cgtaacggca
    20641 ttccgcaatt ccggttaccg caatcagtac tggatgcaga gatcgcccgt attgaaaaaa
    20701 tgggcgtgac cattaagtgc aacaacgaag tcggtaacac actcaccctt gagcagctga
    20761 aagcagaaaa ccgcgcggta ctggtcaccg tggggttatc aagcggttcc gggctaccgc
    20821 tgtttgagca tagtgacgtt gagattgccg tcgacttctt gcaacgtgca cgacaggcgc
    20881 aaggcgatat cagcattcca caaagcgcat taattatcgg cggcggtgat gtcgcgatgg
    20941 acgtagccag cacgctgaaa gttctcggct gtcaggcggt aacttgcgta gcgcgtgaag
    21001 agttagatga gtttccggca agcgaaaaag agtttaccag cgcccgggag ctgggcgttt
    21061 cgatcatcga tggattcacg ccagtagccg tggaaggcaa taaagtcacg tttaagcatg
    21121 tacggttatc gggcgaactg acgatggcgg cagataaaat tattctcgcc gtcggtcagc
    21181 atgccagact agatgccttt gcggagttag agccgcagcg taacaccatc aaaacacaaa
    21241 attaccagac ccgcgacccg caagtctttg ctgctggcga tattgttgag ggagacaaaa
    21301 ccgtggtcta tgccgtgaaa accgggaaag aagccgccga ggcgattcat cactatttag
    21361 agggagcttg ctcatgttaa cgaaagatct ttcgattact ttttgcggcg tgaagtttcc
    21421 caacccgttc tgcctctctt cttcgccggt aggcaactgc tatgagatgt gtgccaaagc
    21481 ctacgacaca ggttggggcg gtgtggtgtt taaaacgatc ggctttttta tcgccaacga
    21541 agtctcgccg cgttttgatc atctggtgaa agaagatacc ggttttatcg gcttcaaaaa
    21601 tatggagcag attgccgagc atccgctgga ggagaatctg gccgccctgc gtcggctgaa
    21661 agaagattac ccggacaaag tattgatcgc ttcgatcatg ggggaaaatg agcagcaatg
    21721 ggaggagctg gcgcgcctgg tgcaagaagc tggcgcggat atgatcgagt gtaacttctc
    21781 ctgtccgcaa atgacttctc atgcgatggg tagcgatgtc gggcaaagcc cggagctggt
    21841 agaaaaatat tgtcgggcag tgaaacgggg ttccacgctg ccaatgctgg cgaagatgac
    21901 gccgaatatc ggtgatatgt gcgaagtggc gctggcggcg aagcgcggcg gcgcagatgg
    21961 cattgcggcg attaacaccg ttaaatccat caccaatatc gatcttaatc agaaaatcgg
    22021 tatgccgatc gttaacggaa aatcaagtat ttccggatat tccggtaaag cggtaaaacc
    22081 gatcgccctg cgcttcattc agcaaatgcg cacccatcca gaactgcgcg atttcccaat
    22141 cagcggtatc ggcggcattg aaacctggga ggatgcggcg gagtttttat tgctcggcgc
    22201 agcaacgtta caggtcacca ccggcattat gcagtacggc tatcgcatcg tagaagatat
    22261 ggcgagcggg ttatcgcatt atctcgccga tcagggattt gattcgttgc aggagatggt
    22321 aggtctggcg aataacaata ttgtcccggc ggaagattta gaccgcagtt atattgtcta
    22381 tccccgtatc aatcttgaca aatgtgttgg ctgtggacgc tgttatattt cctgttacga
    22441 cggcggtcac caggcgatgg aatggagcga gaaaacccgc acaccgcatt gtaataccga
    22501 gaaatgtgtg ggttgtctgc tttgtggtca cgtctgcccg gtgggttgta ttgagctcgg
    22561 ggaagtgaag tttaagaaag gcgagaaaga acacccggta acgttgtaga acccgcgtca
    22621 catccggcat ttggttcctg ttgccggatg cggcgtgaac gccttatccg gcctacaaaa
    22681 ccacacagat catgatgtta tgtgcgttta tgaccgaatg cggaccacat tcacatcatt
    22741 tcttacgcgc gtatttcagt gaatccagcg ctacggcgaa gataataatc gcccctttga
    22801 tgatgtactg ccagtatggg tttacgccga tatacgtcag gccatagttg atgacggtaa
    22861 aaataatcac cccggtcacc acgccaatca ccgtccccac accgccgctg aacgatacac
    22921 cgcctaccac gcacgccgcg atagcatcca gctcatacat aaagccgagg ttgttggtgg
    22981 cagagccgat acgtccggct tctaacatcc cgccaaaggc atagaacacg ccagacaacg
    23041 cgtagatcat cagcaggttc aggccgacgt tgacaccaga tacttttgcc gcttccgggt
    23101 taccgccaat ggcaaaaatg ttcttaccga agcgggtttt gttccacaac acccagacga
    23161 acgccaccgc aatcaacgcg tagaaggtga tgtaagagag acggaaactc cccagcgcga
    23221 caaagccctg agcaaaggta gagaagccac tgtcaaaacc agaaattggc gacgccccga
    23281 caaagtcgta atagagcgag ttgatgccat agacgatgat catcgtgccg agcgtggtaa
    23341 tgaacggcgt cacgttgaga taagcgataa tcagaccgtt gatcaaaccg atcaccgcac
    23401 caatggcaca gacaatcaga ataaccagcg caatcggcat cgtcgccatt tccgggaaca
    23461 ctttgttggc gttatccatg gactgcaata atgtcgcagc caccactgcc gccagcccta
    23521 cctgacgacc agcagaaaga tcggtcccct gggtgacaat taaccctgcc acaccgagcg
    23581 cgataataat acgcaccgat gactgggtga gaatattact taagttcaac agacttaaaa
    23641 atgttgggtc ctggaaaata ataatcgcca gcaaaactaa aagaacgacg taaataccgc
    23701 cctctttcag gtaagtaaga aaacttttct tatttaacgc actcatggga agcccctaat
    23761 cttaaaggtg caaagacgca agacgcagaa tttcgttttg cgttgttgtt tttgtatcga
    23821 caattccgga aacgagacca ttgctcatga ccagaatacg gtctgttatc cctaacaact
    23881 caggcatttc agaggagata ataataatcc ccttgccttt cttcgccagt tcggcaatta
    23941 actgataaat ttcaaacttc gccccgacat caataccgcg cgtcggttca tcgagcatta
    24001 atatttctgg ttgcgttagt agccagcgac caataatcac cttttgctga ttaccaccgg
    24061 agagcgaacc aatttgcgtc cgatgacccg gcgtttttac ccgcatcgaa tcaatcaccc
    24121 actgggtatc gcttttcatc cgcgagttat ccagtaaacc aactttattt ttgtagttgc
    24181 gaatattgga aattaacgag ttaaaaccaa tatccagata ggcataaatt cccgttgagc
    24241 ggcgctcctc agttaccagt gcaaatccgt ggtttatggc ttcgttggca ttatggttat
    24301 tgatctgttt gccgtgcaac gtaatggtgc cagccgattt ctcgcgaata ccaaataacg
    24361 tctcaacaat atcggtacgt ttcgccccca ccagcccggc aataccgagg atctcccctt
    24421 tatgcagatc aaacgagaca tcgcgaatcg acggctggcg cagtgacgtt aggttacgca
    24481 cctcgaggat gacttcgccc ggcttgtttt ctttgtcagg aaaacgctga ttaagagaac
    24541 gcccaaccat catggcgatg atcttgtcca tcgtcagtcc tgccagcggt tcggtggcga
    24601 tccactgacc gtcgcgcaat acggtaactt catcacataa ctggaagatt tcttccattt
    24661 tatgcgagat ataaacaata ccgcagccgc gctcttttaa tttacgaata atagtgaaca
    24721 gatgattgac ctctttttcg gttaacgaag aagtcggttc atccataatc acaattttcg
    24781 cgttatagga aaacgctttg gcgatttcga tcatctgcat ttgcgaaacg gataatgtgc
    24841 cgacgcgcgc acgcggatcg atatcaatat ccagttcatc aaaaattgct ttggtttcgc
    24901 ggtacatttt gtcctgatcg acaaacatgc ctttggtggg atatcgcccc agccacatgt
    24961 tatccatcac cgaacgttgt aataccaggt ttaactcctg gtgtaccatc gaaataccat
    25021 tttccagtgc ctctttggcg gaatggaaat cgatctcttt accctggaat aaaatggtgc
    25081 cggagtcttt ttgataaata ccaaacaggc attttaataa tgtcgatttt cctgcaccgt
    25141 tttcccccat taatgcatgg atagaatgtg gccggacttt taaattaacg ttatcaagtg
    25201 ccttaacacc aggaaaggac ttgttgatac cgctcatttc caacaagtat tccccggaag
    25261 acggagtcgt tgagctgacc atataatttt accttgttgg ccatacaata agggcgcagt
    25321 aaacgactgc gcccaatcag tcttatttct tgctgaattc agccaggttg tctttatcta
    25381 cgccaacata aggtacgcgg accactttgt tgtcgatttt ccagttggtg ccatcagccg
    25441 cacctttgcc atcggccagg tttttcgcca gatcaaaggt cgctttcgcc tggttgttag
    25501 catcgttcag tactgtgccc gccagtgcac cggatttcac cagcgccagc gcttctggca
    25561 gcgcatcgac gccaaacacc ggaatgctgg acttgttgtg tgctttcagt gcttctaccg
    25621 cgcccattgc catcgcatcg ttgttggcga taaccacttc gattttgttg gcgttcgggc
    25681 cagacagcca ggcgtccatc ttatctttcg cctgagcggt gtcccacatt gcggtatcta
    25741 actgtaactg ttcagttttg atgcctttat cgttcaattc tttaatcacg taagtggtac
    25801 gtgcttctgc atccggatgg cccggttcac ctttcagcag tacgaactga atctgaccgt
    25861 ctttgttcag atcccaaccc tgattcgccg cccagtgttt agcaatcaaa tcgccctgaa
    25921 taatgccgga ctctttggag tcagtgccaa cgtagtaggc tttgtcgtag ctatccagcg
    25981 ccttacgaga cggttctttg ttgaagaaaa ccaccggcac gttttgccca cgcgctttct
    26041 caatcaccgt acccgcagct gccgggtcaa ccaggttgat tgccagtgcc ttcacccctt
    26101 tcgccagcaa tacgtcgatc tgatcgttct gcttggactg gtcattctga gaatcattca
    26161 tcagcagctg aacatctggc gcggctttcg catcttgctc aatagccttg cgcactacag
    26221 acataaagtt atcgtcgtac ttatagattg ttacaccaat gcgagtatca gcagcgtgtg
    26281 cagcggcacc gaataacatg ctggccatca cagcagacag ggttaacacc ttcttattca
    26341 tggtatctcc ggtttttctt atgcagggta gtgcttgaga taaatgctcg gcggggcagt
    26401 agagttaatg aagtgttact gaacgccgaa gctcactttt ttaaattcgt tcttccatgc
    26461 ccggtaacgc tccagaaaac ggctttaatt gttgttatga cgctattacc tcggtgaaag
    26521 tgattattca ctgttacata cgggttacaa cgttaaaacg gtgcaatcat agctatcaca
    26581 ttgttaagat actgtgaaat cactcacaga ttgaaagcgg ttacatcgcc tgatttgttg
    26641 agttagtgat cgccgccgca ttctggcgcg ttgcgacaga atggcgacgc actaacgtcg
    26701 gcataaaaca gtggctggca cgaggatcaa tattgcctgc tgccccctgc aaggccagtt
    26761 cggtggctaa tttagccatt gaagcaatgg gataacgcac ggtcgttaat tgcgggtcgg
    26821 tgtaacgggc gatgggaata tcatcgaaac cgatgattga gagatgtaac ggaatcgcaa
    26881 tgccattatc ttttaatgct gtcagtgcgc cagcggccat attgtcgtta taagcaaata
    26941 tagcggtaag ttgtagattg cgccccagca gttcaaccat cgccgcctca ccgcccggca
    27001 tgtccggcgt accagtgcca atccagcttt ccggcggaat aatatcctgc tctttcaacg
    27061 cactcatcca gcctgcttta cgcatggcgt catcttcaat gccgtgactg gaagaaagat
    27121 aaccaatacg ttgatgaccg ttattcagca acatgcgcgt cgccattcgg gcaccgctga
    27181 gattatccag gcaaacgcaa cgatgggcgt accccggcac aacgcggttg attaacacca
    27241 taccgggaat gttatccata aattgcgcca gttcatcgtc actcaatgct tttgagtgaa
    27301 caatcaacgc attacaacgc tggcgaatta acacctcaat ggcgtgacgc tctttttccg
    27361 cttcatgata gctattgccg attagcacgt atttctgatg ctgctgagcg actagatcca
    27421 ccgcttttac cagcgcgccg aaaaacgcat cagaaacgtc catcaccacc acgccaatgg
    27481 tgtcgctaac ctgagttgcc agcgcctggg cattggcgtt tggccgataa tccagctcac
    27541 tcacggcttt cattactgct tcacgcgtgt cggcactgac cagcgtgctg ttattgagca
    27601 cccgggaaac cgttgccaca gagacgccag cctaacgcgc tacatcacga atggtgatca
    27661 tattcactac ctgttatgag attgcagtaa atgactgctt gctggcggct attttgtcag
    27721 cactgaatac aggacttcgt gaatcgagtc acagcaatgg aaacggttac agccgttttg
    27781 ttaacgaatg tgatccagat cgttatcttg atgttttaga tattgccgtt ccggcagcac
    27841 gcaaagttaa ctgacgccag agccattcca ccggcccctg gcggaagtaa cgcagccaga
    27901 taacagagaa gaggatattc gccagccata ccggaataac aaacgccagc agctccaggc
    27961 ggtcaaaatg cataaacaaa ccgaggtggt aaaaaagcat ggtacaaatc agcgtttgca
    28021 atagatagtt ggtcagcgcc atccgtccga cgcaggcgat cgcaagcacc agcttaaagc
    28081 ggctcaattg cggccagaag ccataaaaca gcgacgcata gccaatcgcc tgaaacggcg
    28141 cactcagttc ccgcggcatt tgaagtaaga aggcacacca gcgatacgcc cagtccagct
    28201 gccattgcag ggcgatggca ggaaggttaa tggtcacccc aatcgccacc agcacaaaac
    28261 cagtacgacg atagtgacgt aagctgaact gccctttcag ccagccgctg cgcatcagcg
    28321 ccgcaccaat gagcatcatc cctgccagtt gccagccata ctgtgcgccc agtgccagta
    28381 aactgttgcc aacaccatcg gcacgattac tgatcgcttc aacgccgccg tgaagcttcc
    28441 agtatttttc atacaaaata gccgatgcat ccggcgtcca ggtgcggctg gtctggctgt
    28501 cggaaatcag ccccaataac agcaaaacgc caagccccac cagataaagc atgacgcctg
    28561 tattaaacag gctttttacc gatggcgcat cgcgcaccag ccgccagcag attaagccca
    28621 ccagcccgta agccagcaga atatcgccgt cccagaacaa taaaccgtga ataaaaccca
    28681 gcaagactaa cagcgttaac cgcgactgga tccagcgtct gccacggggc agcaacattt
    28741 gcaggcccgc accaaacagc agcgcaaaaa gcgtgaggaa tttcacctgg ccgatgagat
    28801 cgagaaatgc ccaggtccat gcatcctgcg gcgtaatagc gccgtaccag gcgggattga
    28861 gataagccgc ctttggtagc ccaaaggcgc tgatgtttaa tagcaggatc cccagaatgg
    28921 cgacgccgcg aacaaaatcg agcgttacgt tgcgctccat ggttcctgcc ttttaatcag
    28981 ttgtgatgac gcacagcgcg cagaaactcg tggcgcgtat tctgactgga tttgaacaat
    29041 ccaccaagag aggtcgttgt tgtggcactg gttgcatcgc ggatgccacg cgctttcacg
    29101 cagtaatgca ccgcatcaat ggagacagcc acgttattgg tgcccagcag cgtttgtagc
    29161 gcaataagaa tttgctgcgt cagacgttcc tgcacctgcg gacgctgggc aaagaactgc
    29221 acgatacggt taatttttga cagaccgatc accgaatctt tcgggatata agccaccgtc
    29281 gctttgccat cgatggtaac aaaatggtgt tcacaggtac tggtcagagt gatatcgcgc
    29341 acggtgacca tttcatcgac cttcattttg ttttcaatga gggtgatttt cgggaagttg
    29401 gcgtaatcca gaccggagaa aatttcatcg acatacattt tagcgatgcg atgcggcgtt
    29461 tccatcaaac tgtcatcagc caggtcgaga ttcagcagct gcatgatttc ggtcatatga
    29521 ccagcaataa ggcttttgcg cgtttcgtta tccatttcat gcacgggcgg gcgcagcggt
    29581 gtttccagtc ctcgcgcaac taacgcttca tgaaccaggg ccgcttcttt actgagtgat
    29641 ggcatttatg atttctcctg caggtgtgac gcctccgccc tgcgtggggg caaagttatt
    29701 aagctgattt acagcctgat tattgtgcgt gaggcggcgc acataatcca gtattcacag
    29761 cgataattat tgtaattgcc gctgactttc atcagcagat gttaaaacat cgttatgcaa
    29821 atacggaagt gaaagttact cacagcacat tgaataaacg gtatgatgaa gaaattgcaa
    29881 acaacacaac aaggagccac gcatggaaat gctcgaagag caccgctgtt ttgaaggctg
    29941 gcagcaacgc tggcgacacg actccagtac cttaaactgc ccgatgacgt tcagtatctt
    30001 tctcccaccc cctcgtgatc acaccccgcc accagtgctg tactggcttt ccggattaac
    30061 ctgcaatgac gagaacttca ccaccaaggc gggtgcccag cgcgtggcgg cagaactggg
    30121 gattgtactg gtgatgccag acaccagccc gcgcggcgaa aaggttgcca acgacgatgg
    30181 ctacgattta ggccagggcg caggctttta tcttaatgcc acgcaaccgc cgtgggcgac
    30241 gcattaccgg atgtatgatt atctgcgcga tgaattaccg gcgctggttc agtcgcaatt
    30301 taatgtcagc gaccgctgcg ccattagcgg tcactcaatg ggtggtcacg gtgcgctgat
    30361 tatggcgctg aaaaatccgg gtaaatacac cagcgtttcg gcctttgcgc caattgtgaa
    30421 tccgtgcagc gtcccgtggg gaatcaaagc gtttagcacc tatttaggtg aggacaaaaa
    30481 tgcatggctg gaatgggata gttgcgcgct gatgtatgcc agtaacgcgc aggatgcgat
    30541 cccgacgctt atcgatcagg gcgataatga tcagtttctt gccgaccagt tgcaacctgc
    30601 ggtattggca gaagccgcgc gccagaaagc gtggccgatg acgctgcgta ttcagccggg
    30661 atatgatcac agttactact tcatcgcctc ttttatagag gatcacctgc gcttccatgc
    30721 gcagtattta ctgaagtgaa agtccgcccg gttcgccggg catcttctca tcagaagcga
    30781 taatccactg ccataaagta acgacgtccg tcttcgttat agctgtagtc gtcacgactg
    30841 agatccttgt cgccaaggtt cagcacgcct gcgcgcagtt tgacgtcttt agtcacctgc
    30901 caggccgcgc cggtattcca gatggtataa ccgcccggtg ttttagccgt cgcgctgtcg
    30961 gcgcgtttct gcccggtata gtgaccagaa acatagaatg accagtcttc cagcgccagc
    31021 ggtttccagt ccagcgtacc gttagcagta tggaacggca gatcggatag cggtttgttt
    31081 tcgccgttgc tgacatcacg accatcgttg taggtgtagt tgatcgacag tttccattca
    31141 tcgttgaacg gaattttcag ttcggtttcc acgccctgaa tacgagcttt gttaacgttg
    31201 tagtagctaa ataccggtat gcgccgtccg ttagcgcccg tctcaaaacc aacaaagttt
    31261 tggtagcccg gtgcagcgtt gacgtcagac gtacggctga tgctgatacg atctttcaca
    31321 tcgttacgga aaacggtaac gctggattca accccttcca gccagccttc ttcacccatg
    31381 tagtaaagcc ccagctccca actttcgctg gtttctggtt tcagatccgg gctacccaca
    31441 atcttacatg cgccacggca ggaattgctc gtccagtcag ggctaagttg caacagagaa
    31501 ggtgctttaa atgccgtcgc ccagccccct ttcaccgtta cggtgtcggt ggcgttataa
    31561 accaggtagg cacgcggact ccagtgttca ccgtaggttt cgtgatcgtc catacgcacg
    31621 ccggtcgtca gcgccagcgg ctcgaagatc cgccattcat cttccacaaa cagcgcgtac
    31681 tggctggcag acgttttgga gctggttccc ccggtcaggt tcaccgcatc gctaagtttg
    31741 tcgtgacgcc attcaccgcc aaccgtgaga aactgattaa tcgccgtcag cggcaacgtg
    31801 tatttgccgt cgaccgtatt gctttcggaa gttatcgggc tgctgttgcc agggtttttg
    31861 ttctcgactt tctcaccgta gtatttcagt tcgctggtgc cgtaatccca acgcccatta
    31921 tggctgacgg agtagttctg gcgttccagg cggtttttgt ccagcgagtc ggaatcacga
    31981 tcctgacggt cgaaaccgta tccggcagta aaatcgtgat tttgattcgg tgtccaggca
    32041 aattcgacat tgccgtcgcg gctggagaat ccttcaatac gcggcgtttc tccggtatcg
    32101 gtggtcgttg agttttgcgg gtcatccttt tcacgttttg ccaggctgcc gtaagctttc
    32161 attcccagca caccatcaat taatggtcca ctggtaaaga actgaccgtt ataggtatca
    32221 ccgcgatcgc gatgttcctg aatggtggta tcgacggtaa cggtacccga ccatttctga
    32281 ccgatttttt tggtgatgat attcactaca ccgccgagcg catcggaacc gtacagcgac
    32341 gacatcgggc cacggaccac ttcaatacgt tcgatggaat cgaccgggat ccagttcaga
    32401 tcgaaatcat tgtggcggaa gacggcattg cgggagttca cgcgtttacc gtcgacgaga
    32461 atcagggtat agctgctgtc cagaccacga atactaacgc ccttacggtt atccccttcg
    32521 ttcgtcagtt gtacgccagg cacttctttg aggacatcct tcagattctg taccggtttt
    32581 cgctgcagat cttcctgggt aatgacgctg atactggcgg gtgcatcttt aaggttttgt
    32641 tcaacggaag atgcagtgac aaccatcgtt tcgccatcgt catcgaccgc taacacaggc
    32701 catacacaag aaatagcgga caaacacagc ccgacccgta cgacagggtt caacctaaac
    32761 attccatatc tccatgaggt aactacgaaa ataaaatggg tatcgctcac atcttcttta
    32821 cgtccccttg cgtacggcag catcgcggtg gacttatttg tatgcaggtg atcatcccag
    32881 aaagccggac cagcctctga tttgtcagcc ttttttgatt gtagcgtaac gataatgcaa
    32941 acgataacaa ttatcaatcc aaatgttaaa ttttatatcc gcatgatttg tcagggaaat
    33001 ggaattgaaa aagccctctc ggttgagagg gcttagcaag gaagggagga aacttatttc
    33061 ttatcgttct gcgggaactt catttcgctg tagcgtacga agtgagttcc tttaatcagc
    33121 ttgtagccga accaaataat caggaacagc gggataccaa tatacgttgc cgctacgccg
    33181 ccccagtcaa tggtatcttt caggaacgct tcgtagttct ggcccaaagt gataatcaga
    33241 cacagaatga atgcgaagat cggccccagt gggaagaaac ctgaacggta cggcagatcg
    33301 ttaatgtcgt gtccctgcaa tacgtaaccg cgacggaagc gatagtggct aatggcaatc
    33361 cccagccagg cgataaaacc cgtcatcccg gaggtgttca gcagccacag gtataccgtc
    33421 tggttgccaa acatggaggt caggaagcac aaaccggcaa tcaccgtcgt cgcatacagc
    33481 gcattacgcg gcacgccacc acgcgacaat ttagcgaaaa tgcgcggcgc tttaccgtca
    33541 cacgccaggg tgtacagcat acgagtagac gcatacatac cggagttacc tgctgacagc
    33601 accgccgtca gaataactgc gttcatcacc gccgccgcag agagcagacc cgcgtgctgg
    33661 aacaccaagg tgaacggact gacgctgatg tctttaacat cgttacgcag caggctcgga
    33721 tcggtatacg gaataatcag gctgataatc aggatcgcga acacatagaa caacaggatt
    33781 cgccagaata cctgacgtac cgcgcgtgga atgtttttcg ccggatcttc ggactcgcct
    33841 gcagcaatac cgatcagctc ggttccctgg aaagagaagc cgacaatcat agctacgccg
    33901 atcatcgccg caaaaccacc agcaaacggc gcttcgccga ttgtccagtt gctccagccc
    33961 gcaggctgcg cgcctttgaa gataccgata atcatcagca cgccaacgat gataaagaca
    34021 ataactgtcg tgactttgat cagtgaaaac cagtattccg cttcaccaaa gccacgaact
    34081 gagatgtagt tcagcaggaa gataacgccg aggaacaacg cactccagat ccagcccggt
    34141 gtatccggga accaccagct catgaccagc tgtgctgcaa ccaagtcaac ggcaatagtc
    34201 accgcccagt tgtaccagta gttccagccc agcgcgaagc caaagccttc ttcaacatag
    34261 ttctgaccgt aagtggcaaa cgaaccggaa accggcatat atgcagccaa ttcaccgaga
    34321 ctggtcatca ggaagtaaac catcaggcca atcagcatat acgagagcaa tgccccgccc
    34381 ggacctgcct gagaaatcgt tgcgccagag gcaacaaaaa gacctgtacc gatggaaccg
    34441 ccaatggcaa tcatcgtcag gtgacgcgcc tttaattcac ggcgtaagcc cggcgcttct
    34501 gtagttttag tttcggaacc catacaaaaa tgctatccat cttaaaaatg aggcgcgatt
    34561 gtagcagacg attggcaatc cttccggcag aaatacacgg ttataagaca ccttcatgat
    34621 cgcccaggga ttataagtaa agcagccaat ctcttttctg gcgaatgcat gctgagggcg
    34681 ctgcgtcacg aaatcgacac agcaccagca tgttcttgta cagcacctta acgcggtaca
    34741 ttcgcgggat cgcaatagtc cagaaagcgc cgtagcgcgt tggaaaggtg tttttgccga
    34801 tgatgtatac gccacaacgt acgcatcagg cgcgggagag ggaccgcaac ttcacttaat
    34861 gtgcctgcct gcaattgatc ttcaatcaca cgtcgcgaca ggcaactaat tcccaaccca
    34921 tgacgcaccg catgtttgat tgcctcggag ttacctaatt ccatcgccat ctcaaacttc
    34981 ggtaaatgtg acagcaacag ataatcgaca atctcccgcg tgccggaacc gcgttcacgc
    35041 aggatccacg gcgcagcggc cagctgttct aaggtgaccg gaccacgagc caacggcgaa
    35101 gtcggcgcgg cgaaaaccac cagctcgtct tccagccacg gttcagaaat gatttcagtg
    35161 ctgtggcacg gtccttcaat aaagccaata tcaacgcgga aatccagcac cgcttgcatc
    35221 acgtcctggc tgttcccaac gctaagttca atcggcaact gcggataatc atggcgataa
    35281 cgggcgataa ctgcaggcag aatgtagtta ccgatggtac tactggcata gatacgaatc
    35341 gcgccgttgt cttcgcgaaa cagttgttcg atttcaaccg cctgttcaag caatgccaac
    35401 gcacgcggat agagcagccg cccgtgttca ttaacaacca gtcttttccc cacgcgatca
    35461 aacagttgca cgccaagctg cccttccagg tcggtcaagg ctgcgctcac tgctgattgc
    35521 gacaacgcca gcatcaccga cgcctgggtg gttgatccac ttttcaatac ttctgcaaaa
    35581 acttccaact gccggagggt gatgtgcata gtcgcttacc acttataaag attaattata
    35641 aatatataat caattttatt tttaaaccag ttagtcgtaa ccttatgccc ggttaaagga
    35701 gagggttatg acgaatatca ccttacagaa acaacatcgt acactgtggc attttattcc
    35761 ggggttagcc ctgagtgcag ttatcaccgg ggtcgccctg tggggtggtt ccattcccgc
    35821 ggttgcgggt gccgggttta gtgccctcac cctcgcaatc ttgttgggga tggttttagg
    35881 caacaccatc tatccgcaca tctggaaaag ctgtgacggt ggtgtgctgt ttgccaagca
    35941 atatttatta cgtctgggta tcattcttta tggcttccgt ctgacgttct cgcaaattgc
    36001 cgatgtcggt atcagtggga tcatcattga cgtgttgacg ctgtccagta ccttcctgct
    36061 ggcttgcttc ctggggcaga aagtgtttgg tctggataag cacaccagct ggttgatcgg
    36121 tgccggtagc agtatctgtg gtgctgccgc ggtactggcg actgagtcgg tagtgaaagc
    36181 ggaagccagt aaagtaaccg tggctgttgc aaccgttgtt atcttcggga ccgtcgcgat
    36241 tttcctctac ccggcgatat atccgctgat gtctcaatgg tttagtccgg aaactttcgg
    36301 tatctacatt ggttctactg tgcacgaagt ggcgcaggtg gtggcggcag gtcatgccat
    36361 cagcccggat gcggaaaacg cagcagttat ttccaaaatg ctgcgcgtga tgatgctggc
    36421 tccgttcctc atcctgctgg cggcgcgtgt taaacagctg tctggggcga acagcggcga
    36481 gaaaagcaaa atcactattc cgtggtttgc catcttgttc atcgtcgttg ccatctttaa
    36541 ctcgttccac ctgttaccgc agagcgtggt gaacatgctg gtaacgctgg ataccttcct
    36601 gctggcaatg gcgatggcgg cgctgggtct gaccactcac gtcagcgcgc tgaaaaaagc
    36661 tggggcgaaa cctctgctga tggcactggt actgtttgcc tggctgattg ttggtggtgg
    36721 tgcgattaac tatgtgattc aaagcgtcat cgcataaacc actacatctt gctcctgtta
    36781 acccgctatc attaccgttt tcctccagcg ggtttaacag gagtcctcgc atgaaataca
    36841 ttggagcgca cgttagtgct gctggcggtc tggcaaatgc cgcaattcgc gccgtcgaaa
    36901 tcgacgcaac cgcgtttgcc ttgttcacca aaaaccaacg tcagtggcgt gccgcaccgc
    36961 tcacgacgca aaccatcgat gaattcaaag ccgcctgtga aaaatatcac tacacatcgg
    37021 cgcaaattct tccccacgac agttatctga ttaacctcgg acatccggtc actgaagctc
    37081 tggaaaaatc gcgcgatgcc tttatagatg aaatgcagcg ttgcgaacaa ctggggcttt
    37141 ctttgctcaa cttccaccct ggcagccatc tgatgcagat ttcagaagag gattgccttg
    37201 cgcgtattgc cgaatccatc aacattgcgc tggataaaac tcaaggtgta acagcggtga
    37261 ttgaaaacac cgccggtcag ggcagtaact tagggtttaa attcgaacat ctcgcggcga
    37321 ttatcgacgg cgtggaagat aaattccgcg tcggcgtctg cattgatacc tgccatgctt
    37381 tcgctgccgg gtatgatttg cgtactccag ccgaatgcga aaaaacgttc gctgattttg
    37441 cccgtattgt cggctttaag tatctgcgcg ggatgcacct taacgatgcg aaaagtacct
    37501 ttggcagccg cgttgaccgc catcatagcc tcggtgaagg caatatcggt catgatgcgt
    37561 tccgctggat catgcaagac gaccgtttcg acggcattcc gctgatcctt gaaaccatca
    37621 acccggatat ctgggcagaa gagatcgcct ggctgaaagc gcaacaaact gaaaaagcgg
    37681 tggcctgatc gatgaacgac agggaaaagc agattcttaa aattttacgg cgtaacccgc
    37741 tgattcaaca gaatgaaatc gccgatattc tgcaaatcag ccgctcacgt gttgccgctc
    37801 atattatgga tttgatgcgc aagggtttga ttaaaggcaa aggatatatc cttaccgagc
    37861 aggattactg tgtggttgtg ggtgcaatca atatggatat tcgcggaatg gcggatatcc
    37921 gttacccgca agcagcctct catccaggtt ctgttcactg ttccgcaggc ggcgtcggac
    37981 gtaatatcgc gcataacctg gctctgcttg gtcgggatgt gcatttaata tcagctattg
    38041 gcaacgattt ttacggcgag acattactgg aagaaacgcg ccgcgcgggc gtgaacgttt
    38101 caaactgcat tcgtctgcat ggacacagca ctgcaactta tctcgctatc gctaataagc
    38161 aggaagaaac tatcctggca atcaatgaca cacatattct gcaacagcta acgcctcaac
    38221 tgctcaatac ttcacgggac ttgattcgcc atgctggtgt ggtgctggct gattgtaatc
    38281 tcacacctga agccctggag tgggtcttta ccattgctga tgaaattccg atgtttgtcg
    38341 ataccgtttc cgaatttaaa gcgaacaaag taaaaaactg gtacagccgg atccacactc
    38401 tgaaaccaac acaaaatgaa ctggaaattt tgtgggggca accgattaaa gacgacaacg
    38461 atcgcatcag agctgtaaat tcgctgcatc aacaaggtgt taaacgcatt tttgtttatc
    38521 tgaaagatga atctgtattt tgcagcgata aggatggaga acaatttttg ctgaccgcgc
    38581 cagcgcatac gacagtagac agttttggtg ctgacgatgg ttttatggcg ggcctggtat
    38641 atagctttct tgaaggatac agtttccgcg acagcgcccg ttttgcggta gcctgcgcgg
    38701 caatttcgcg cgccagcggc agcttaaaca accctaccct gtctgccgat aacgcgcttt
    38761 cattagtgcc aatggtgtaa caatgttgcc ggattcgacg cttgaccgca tcttatccgg
    38821 ctcataaatt gcgcactacg ctaaaccaat aaagaatcct gcaatagtag cactcatcag
    38881 gttagaaagt gtcgccgccg ccagcgcgcg taaaccaagt tgggcgattt ccggcgcacg
    38941 gtgtggcgca accgcagaaa acgcccccac caccaccccg atagaaccaa agttagcgaa
    39001 accgcacaac gcgaaagaaa taatcgccac ggttttagca tccagagtgc cacccgtttg
    39061 cagatatggc gagaaattga gataagcgac aaattcattg atcgccagct tctgcccaat
    39121 caaactcccg gcaagatttg catcactcca gtcaaccccc atcacccacg ccaatggggc
    39181 caacaggtaa cctaaaatcg actccagcga agcatgttca aaaccaaacc agccgccaac
    39241 accgccgata ataccgttaa tcaacgcaat tattgcaaca aatgccataa caactgtcgc
    39301 tacaccggcg gcgattttca gcccggtcat tgcccctgtc gcagcggctt caataatgct
    39361 ttttggcggt gtttcggtga aagagaggtt attaaaagaa acctgcgaag attccgtagc
    39421 cgggcttaac aggcgggcaa acaagatccc gccagggatc gccattaacg atgccgccag
    39481 caaatattcc acaggtacgc ccagtgcggc gtaaccaatc attgtcgaac cagcaatcga
    39541 ggccatgcca ctacaaatcg ctgtaaataa ttcattgcga ttcagacgat cgataaaggg
    39601 tttgacgatc gccggaattt cgttttgccc gaggaaaatg gtggtgaccg cgacgaatga
    39661 ctcgatcttg ctgatattta aagctttctg gaagataccg ccgagaatgc gaattaagat
    39721 ccccatcaca ccgatgtagt agagaatact caccagtgca gtgacgaaga taattgccgg
    39781 taataccctg aaaccaaaga taaatcctgc gccatcaaat aaggtatcca ttttcggtcc
    39841 gaccagagaa ccgaagataa atgcgctacc tgcgtcgctg tacgccatca ctttgtggac
    39901 gccaaaagcg actttttctg cgacccaacg ccctggcggt aaccaaagca taatcccgcc
    39961 aatcacgacc tgtaacacta acgccgcgcc aacggtacgc aggctgatct tcttcttgtt
    40021 tactgacagt aaaaacgcaa ttgccagcag taccaccatt cccagaacac ttctcatgac
    40081 atccatagtg attatctctt catgtcattt tgggcgacgg ctttctgccc atcagaaata
    40141 gccgggcctg catcttgcga tactgtcccg gcttgttatc aacacttaat gggtttttat
    40201 gtagccgcgt acgcactctt cgactaatcc ccagaaccag tcggtatcaa tagtgatgcc
    40261 gactttagta ttggcgggct tgccaagaac gcccagctcg tcgcagacgg tacgcccata
    40321 gcaagggcca ctgttgacgt ccacttcgac gtacatctcc tgggttttaa tgccatcagg
    40381 gttaatcaga taaccgatgc aggtggcgtc gtgcaccggg ccgccagcca ggccgtagtt
    40441 ttcgaactgc gttttgagag tgaagttcat gatgtcgctg aacatctctc cggcagggcc
    40501 gcctgccctt tccattcgtg caattacgtc cggggtgcaa acggtctggt tggtaagatc
    40561 gaggcccatc atcactaatg gaacgccgga ggtgaacacc acgcgtgcgg cttccgggtc
    40621 ggcaaagata ttgaactcgg cagatggggt gaagttgcct gtaccgtaag cgccgcccat
    40681 tagcacaatt tcacggattt tgggcaggat tgcgggttgc atacgcattg ccaccgcgat
    40741 atttgaaagc ggaccaaccg gcaccagagt gatatcgcca tcgcttgcca taagggtatc
    40801 aatgatgtat ttcaccgcgt gggtactttc tgcctggcgg gtcagcggct cgaataccgg
    40861 gccatccagt ccggtttcac cgtgaatatt atcggcaacg atttgttgac gcataatggg
    40921 ttgcggcatc cccgcataaa ccggaacatt aatctccagt ttctggcaaa cattcaggcc
    40981 attaattaat gttttatcaa gcgtctgatt acccgcaaca atggtgatac ctaataaatc
    41041 aatcgccgga tgtttcgccg ccatcattat agcaatagca tcatcatgac ccggatcaca
    41101 atccagaata atttttctct tttccattgt ttatttcctc tgtttccagt tgcgttattt
    41161 tttctacagc aacgaaattt cgatcgggcc gatgattgaa tcttaacaac agcgtacgaa
    41221 tgctaaatat gagaaatctc atagcggata aacatcgtga aagaaatcca caataatgat
    41281 cttaagcagc aattgatgag tgaatccgcg tttaaggatt gctttttaac ggatgtttca
    41341 gccgatacgc ggctgtttca ttttttagcg cgtgactaca ttgtgcagga agggcaacag
    41401 ccgtcctggc tgttttacct gacgcgaggc cgcgccaggc tttacgccac gctagctaat
    41461 ggtcgcgtgt cgctgatcga tttctttgcc gccccctgtt ttattggcga gattgagtta
    41521 atcgacaaag accatgaacc gcgtgcggtg caggctattg aagagtgttg gtgccttgcg
    41581 ctccctatga aacattaccg cccgctgtta ttaaacgaca cgctattttt acgaaaactc
    41641 tgcgtcacct taagtcataa aaattatcgt aatattgttt ctttaactca gaatcaatca
    41701 tttccgttag tgaatcgcct ggcagcattt atattactct cgcaggaagg tgatctttat
    41761 cacgaaaagc atacgcaagc ggcagagtat ttaggcgttt cttatcgaca tcttttatat
    41821 gttctcgcgc aattcattca cgacggttta ttaattaaaa gcaagaaagg gtatctaatt
    41881 aaaaacagaa agcagttgtc aggactggcg ctggagatgg acccggagaa taaattctcc
    41941 gggatgatgc agtaaaaatt attggcaata gcacgattgc cggatgcaac gcttaataag
    42001 gcgtacatcc ggcaatgaaa ctcaagctaa accaataaag aacccggcaa tggtcgcgct
    42061 catcaggttg gaaagcgtcg ccgccgccag cgcccgtaaa ccaagctggg cgatttccgg
    42121 cgcgcgtttt ggcgaaatag ccgaaaacgc tccaacgaca acaccgatag aaccaaagtt
    42181 tgcaaaccca cagagggcga acgagataat ggcaatggtc ttgacatcca gcgtgccgct
    42241 cgtttgcaga tagggcgaga aattgagata agcaacaaac tcattaatcg ccagtttctg
    42301 cccaatcaaa cttccggcaa gatttgcatc actccagtcc acccccataa tccatgccaa
    42361 cggggccagc acatagccga aaatgctttc cagagagaca ttggcaaaac caaaccagcc
    42421 gccaactcca ccaataatgc cgttgatcag cgcaatgatc gctacaaatg ccattaccac
    42481 cgtcgccaca ccggcggcga ttttcagccc ggtcattgca ccgttcgccg cagcttcaat
    42541 aatgcttttt ggcggtgttt cgctaaacga cagattttca aacgtaactt gcgaaggttc
    42601 cgtcgccgga ctaagaatac gtgcaaacag gataccgcca ggaatagcca tcagcgaagc
    42661 ggctaacaaa tagtcaatcg gcacccccat tccggcatag ccaatcatca tcgaacccgc
    42721 aatagacgcc atgccgctac aaatagccgt aaatagctcg ttgcgattca tgcgatcaat
    42781 aaaaggttta acgatcgccg ggatctcatt ttgcccgagg aaaatagtgg tgactgcgac
    42841 aaaagattcg attttgctga tgttgagggc tttctgaaaa atactgccaa gaatacgaat
    42901 cagcagcccc attacgccaa tgtagtacag caagctgata agcgcggtaa cgaaaataat
    42961 cgccgggagt acgcgaaagg cgaagataaa gcccgcaccg tcaaacagaa catccatttt
    43021 gggaccgacc agcgaaccaa aaataaaggc acttcctgcg tcactgtagg acatcacttt
    43081 atggacgcct aatgccgcct gctctactgc ccattttccc gggggaaaat agagcatgat
    43141 gccaccaatg gcaatttgca gcagcagcgc agcgccaacc gtgcgcaagc tgatgctctt
    43201 tttatttacc gacaacacaa aggcaattgc cagcaatacc accatccccg caacacttct
    43261 cattatatcc ataatgattt tcccttcatg ccagtaaacc cggcgtcagc gccaggtttt
    43321 ggtatgtttg atgagtacgg gcgacggctt tctgcccgcc agaaattaac ccacgagacg
    43381 ctgatattct ttggcaattt cgctcgccag aatggcgtta ttaaagacca gttgaatatt
    43441 ggatttcagg ctatcaccgc cggttagctc cgcaacacgc gccagcagga acggtgtact
    43501 ctctttccca ataacaccct gttcttcggc ttcagcaacg gcttgatcaa ttgctgcatt
    43561 gatggtttct tctggcatcg caaactgttc cgggatcggg ttcgctacca ccaggccgcc
    43621 gttcaaaccg gtttgccact tcactgccat cgcgcgagca atttcgcggg cactgtcaag
    43681 acgaatgctg acttcaaatg ggctggttcg gcagaaaaac gcaggcagcg cttttgtctg
    43741 ataaccaatt aacggtacac cgaaggtttc taaatattct gttgttaatc ctaaatcgag
    43801 aatagatttt gcccccgcac aaacaacagt gacattggta tttgccagtt cctgtaaatc
    43861 ggcagaaata tcgaaggtat gttccgctcc gcgatgcaca ccaccaatac ctccggttgc
    43921 aaaaacttta atcccagcca gtgcagcaat aatcatggtt gatgcaacgg tagtcgcgcc
    43981 atttttccct gcagcaacga caaaaggtaa atcacgacga ctaactttcg tcacgttatg
    44041 tccttcacgg cccagcaatt cgatttcttc tttacttaag cccactttca tcacaccgcc
    44101 aataatggcg atagtggctg gtactgcgcc ctgtttacga attgtttctt ctacttcaat
    44161 tgcggtttgg gcattttgtg ggaatggcat tccgtgagaa attatggtcg attccagcgc
    44221 aacaaccggt tttttatttt ttaaagcatc ctgtacttca gcggatattt gtaataattc
    44281 aggggacaga gttaattcag acattctgtg ttctccacta acgatctaac attggcaatt
    44341 gataattcag ggttgttggt atattcacag gcaagtgcca ttgacgaaca tccctgcgca
    44401 aatcgaacag aatcgataaa cggcataccg tctacccaac aggaggccag ccccgccatc
    44461 atggcgtcgc ctgccccagt aacattgatg acattcgttt taataggggc cgaccaccca
    44521 ctttcaccgt tgatatcact gtagtaaacg ccatcccctc ccatgctcag caccaagcgg
    44581 ttcaggccgt gttgatgaaa ccaggcggcg acctttgcga catcttcacg tccggaaagt
    44641 gcaattccgc tcaacgtttc ggcttcaaga cggttaggtt ttaacgtatg gattttgctc
    44701 agatgatcgc ggattttgac gcatttccat gccgataccg gatcaataaa tacaggcgtt
    44761 tccccgctat tttccagaac ccagaccagc acctcttcac taaggttgca atccgcgaca
    44821 ataacctttg caccgcgaat aaactcctga tgctgggcaa gaaattctgc agaaatacag
    44881 tcactaatac tcatatcatt tatcgcgaca agcatttctc ctgtattatc gagcagtgat
    44941 aaatagctgg atgtattctc acccggaaca attaagcatt tatcgacata aacaccggat
    45001 tgattagttt gcgccagtaa tgattgccca taaaaatcac cacctactgc acttaaaagc
    45061 caggcatttt tccccagtaa cgccagatta tgagcgatat tccttcccac gccacctggg
    45121 gtaaatttaa tttttccggg attcgaatcc gcataattca atggatgatg tgaataccca
    45181 gccacatcaa tattcgccga accaataatt actatgtagt ccttttcgcg catagcacgc
    45241 cccctggcaa taaattgcca acgaagaaat tgggtaaaat agccattaca gtcaataaga
    45301 ctattagagc acgtgttcag atttaaacac atgttcataa taagcatatt taacgtaatg
    45361 taaatcgatc agaaaaaacg aaatcggcca gatatgaagg cggtcacaaa gtttgtctat
    45421 ttatgcagca acaaaagaga aagttgtgat gtgtaacaca caatatggca ggcaagaaaa
    45481 agggcagaga tgtttctgcc ctccagattg ttgacaacgc gcgctttgtt catcctggat
    45541 gtggcgtaaa cgccttatcc gacctacaaa agcgcaaaaa attcaatata ttgcagggtt
    45601 catgtaggtc tgataagcat agcgcatcag gcaattttgc gtttgtcatc atcggggcag
    45661 agatgtctca tccccgataa tgaagataaa ttatgcgttt ttttctacta tttgtgtttc
    45721 cgggcgtttc aggaaggcat aggccaaacc cgccaccagc gtaccggcaa taattgctac
    45781 caggtaaccc aataccggcg taatagcgcc agggatcagc agaacaaaca gaccaccgtg
    45841 tggtgccatc agtttcgcac caatcgccat tgagattgcg ccagtcagcg ccccaccaac
    45901 gatacagcac ggtagcacac gcatcggatc acgagcagca aacggaattg caccttccga
    45961 aataaagcac agaccaagta ccagagcggc tttgccacct tcctgctgcg ctttgtcgaa
    46021 tttgcgacgc gccaccattg ttgccagacc cattgccagc ggtggcacca tacctgccgc
    46081 cataatcgcc gccatcgggc cataggtttg agtactcagc agacccacac cgaatgcgta
    46141 cgctgctttg tttaccggac cgcccatgtc agtacacatc atgccaccga ggatcgcccc
    46201 cagcagaacc gcattcgcag tccccatggt ctgcagccag tgagtcaacc catcgagaat
    46261 gccagcaacc ggtttaccga ttaggtagat catcgccaga ccgaccacca gactggaaat
    46321 tagcggaatg atcaggatcg gtttcagcgc ctccatactc tgtggcagtt tcaattgcgt
    46381 actgattaac ttcgcaatgt aaccagccag gaagccagca attataccgc caataaagcc
    46441 agaaccggtg ctgaccgcca gcataccgcc aatcagaccc ggtgtgaggc ccggacgatc
    46501 ggcaatggag aaggcaatat aacctgccag taccggcacc atcagcgcaa aggctgaacc
    46561 accaccaatc tgcatcagcg ccgctgccaa cgtacccggc tctttaaacg cttcgatacc
    46621 aaaagcaaaa gaaagcgcga tacacagacc acctgcaaca accatcggca gcatgtaaga
    46681 gacgcccgtc agcaagtgac ggtaagcgcc tgcactctct ttcttacctt cagtggtcgc
    46741 tgtttgagct ttgcccgctg gttcatacgg cgttgcttca gcaaccgctt tatccagttc
    46801 ctgcgcagtt ttcttcagcg ccagaccggt agaggtgcga tacatcggtt taccagcaaa
    46861 tttcgccaga tccacttcga tatctgccgc cacaatcacc agatccgctg ctgcgacttc
    46921 ttcaggggtg attgcattgc ccgcgccaac agaaccacgg gtttcaactt tcacccacca
    46981 gccacgtttt ttcgcttcgg tttcaatggc ttcagccgcc ataaaggtgt gtgctacgcc
    47041 agtcgggcaa gcagtcaccg caactacgcg tttcggaccg ctggcagcaa ccggtgctgt
    47101 cgcagcgacc ggcgcagtgt aaggtttcgc atggcctttg gcttcactca ggaacagctc
    47161 agggtgcgca actgcccggg aaatatcacc cagccagaca tttttaccgt tcagcgcgct
    47221 gtcattcggg atggaatcac cgagaacaat cgccatttct gcgtcgttcg gattgtcgat
    47281 gatttccagt tttgcttttc gcgccgccgc gcccagcagg gtcttcgcca tataggcgcg
    47341 tgcctgaccg agattagcgt caataatcag cagcgttttc attatgcctc tcctgctgtc
    47401 agttaaaagg ttgtaagtcg acgcgcgcca tcattgcggc caactgcgga cgatcggtaa
    47461 tacccacatt gctttgactt accgccaggg ctgcaacagc tgtcgccaga cgcagtgtgt
    47521 gttcactgga ttcacgcatc agcaagccat aaatcaggcc accaaccata gaatcccctg
    47581 cgccaacggt gcttacgaca tcgactgacg gtggtttggc gatccattcg ccggaggcat
    47641 taacccaaag cgcgccttcg gcacccagtg aaataacaac atgcgcaata ccttgttcac
    47701 gtagcgcatg tgcagcttca atcacatctt tcatttcagg cagtttacgg cctgcccaga
    47761 tttccaactc gcggcggtta ggtttcacca gccacggtgc cgctttcaaa cctgctacta
    47821 acgcttcacg gctactatca aagataatgc aaggacactg actacgcagg cgagtcatcc
    47881 agtcggtgaa cgcttccggg ctgacgcctg acggtaagct tccgctgaca cagaccatat
    47941 cgaactgacc gagccagctc agagaatcag tcacaaagcg ttcccagtcg gcgagggtga
    48001 cttcaaaacc cgagaagttg aagtcggtca cttcgccgtc tttttccgtc agcttaacgt
    48061 taattcgggt gcgcccctgt acaacctgga aacggttggc aatgcccagc tcgctgaaca
    48121 gttgctgaaa accatcctga ttgtctttac ccaggaagcc gccaacggtg acatcaattc
    48181 ccaggtcttt taatactttg gccacgttga tgcctttacc cgccgcatgc agaccggtgg
    48241 ttttcaccag gttcacttcg ccgcgttcaa tttccgggca gaaaccaaca aggtcataag
    48301 ccggattaag ggtgatcgta gcaacacgtc tgctcattat gcgccctccc caagaccagc
    48361 agcgatagcg tcgccaatgg ctttcagcgc ctgttcagca tctgcaccct gggcggtaaa
    48421 gcgtaggcga tgacctttct taacgccaag tgccacaact ttcatcagac tacgtccgtt
    48481 tgccggtttg ccggtgccat caaggtttgt cacggtaata tcactgttaa attgtttaat
    48541 ggtattgacc agcatggtac ctggacgagc atgcaggccg tgttcattgc gcaccacaaa
    48601 ctccgcgctt aacacgtcgt cggtcggcgc atcatcgctg gtcagcagcg ccagcaacgt
    48661 tgccgcatcc gctttcagca agcggtcagc tttattgtcg agcaataaat cagcgagacg
    48721 cttaagaacc gcgatgggct gatcgtcatt catcgccaca ctcaccagca tggctgccgt
    48781 ttcgccgtcc acatcaaaag catttgccgc acggcttacc gcaatcgcgc tacgcagatt
    48841 gccttcggcg ctatcgctca gccagatacc ctgtccgaga ttcagcggtt gttcattgat
    48901 ggctttggtg acgaaagtgg cgtcaactgc ccccacctct ttcagacgcg cagcgttcag
    48961 cgcctgaaga gtcagcagat cgctggcgac gatatccagc gtcagcattt cgttgtcgag
    49021 cttcagctgc tcactctgct tttcgcccat cagtaatgcg cgaagttctt ctgctgttgt
    49081 tgctgacttc agttgttcag caacggaatc atcgctcagt acgtgggtca gctggcgtag
    49141 caggcccaga tgttcatccg agctggcagc aataccgatt gccacgtacg ctacctgacc
    49201 gtcaccccag gtgacgcctt ccgggaactg aaatacctga acgccggttt tcagcacctg
    49261 atcgcgggtg tcggtagtgc cgtgtggaat agcaatacca ttgccgagga acgttgaggt
    49321 ttgctgttcg cgcgccagca tgccattgac gtagccttct gctacattac cggcctgcac
    49381 cagcgccgca gcgacctggc gaatcgcctc ttctttgtct ccggcctttt cgcccggatg
    49441 gatgtcctgt acggataact ggaacatagt tctcctctct tgctgaattg aaacgattca
    49501 gcctctatga gaaaaaaagc gccaacctgg cttagggtta aagacaagat cgcgctgaaa
    49561 cgtttcaaga aagcataata cttctgtttc agcacgcaag gaaagccgta aagttatgag
    49621 caaaagtttg atgtgctgca caattttttg tcatttttcc taattgctga tgggaaaatc
    49681 cgttatcagc aatttcattt cagcagcgtc agcagccaat cagcaacagt gaaatggcat
    49741 tttgattatt cgtggcgctt ttgatttcgc tgtctgttta ttttctgaca agcagcgtaa
    49801 actccgcgtc ttcctcttcc agtgatcgac ctgcatgcat aactcccccg cagtctccag
    49861 cgcgaaatcg tttgacctga cctcgacggc gtttttaatc gttgcctttc tcaccggtat
    49921 tgcgggtgct ctgcaaaccc cgacactcag tattttcctt accgatgaag tacatgcccg
    49981 tccggcgatg gtgggattct tctttaccgg cagcgctgtc attgggattc tggtaagtca
    50041 gtttctcgct gggcgctctg ataagcgcgg cgatcgcaaa tcgctgattg tcttttgctg
    50101 cctgttaggc gtgctggcct gcaccctttt tgcctggaat cgcaactact ttgttttgct
    50161 attcgttggc gtctttctta gcagctttgg ctcgaccgct aacccgcaaa tgtttgccct
    50221 tgcccgtgaa catgccgaca aaaccggacg tgaggcggtg atgttcagct cttttttacg
    50281 cgctcaggtt tcactggcat gggtcattgg cccaccgctg gcttatgcgt tagcgatggg
    50341 tttcagcttt acggtaatgt atctgagcgc agcggtagcg tttattgttt gcggcgtgat
    50401 ggtgtggctg tttttaccgt cgatgcaaaa agagcttccg ctggcgaccg gcacggttga
    50461 agcgccgcgc cgtaaccgtc gcgatacgct actgctgttt gtcatttgta cattgatgtg
    50521 gggctcgaac agcctgtaca tcatcaacat gccgctattt attatcaacg aactgcatct
    50581 tcccgagaaa ctggccgatg tgatgatggg gaccgccgcc gggctggaaa tcccgacgat
    50641 gttgattgcc ggatatttcg ccaaacgtct gggtaagcgt ttcttaatgc gcgttgctgc
    50701 cgtgggtggc gtctgttttt acgcaggaat gctgatggcg cattcacctg tcattctgtt
    50761 gggcttgcag ctgctaaatg ctatttttat tggcattctg ggcggcatcg ggatgctcta
    50821 ttttcaggat ctgatgcccg gtcaggcggg ttcagccacc acgctctata ccaacacttc
    50881 gcgcgtgggc tggatcatcg caggatcagt ggcgggcatc gtcgccgaaa tctggaatta
    50941 tcacgctgtg ttctggtttg cgatggtgat gattatcgcc actctgtttt gcttactgcg
    51001 gattaaagat gtttaaggcg cggtcagcat ttccagatcg ataagccagg tcatggcctg
    51061 ctgacgcgag ttaccgcaca tttcagcgct ggcttgtaag cctgcacaga ctttcggtcg
    51121 cagtggcgag gtgaaaattt tacagcgttg ctgttcatca agctgaatgc agggcgtatt
    51181 ggcgggcttg ccatctggca taccgggaat agggctggaa attgaagggg cagtgcaaca
    51241 cgccccgcaa cccggacggc attccatacg ttgttctctt aaggttaacc accggattga
    51301 tgagcgcgca cagtaccacc ttttttgcac cagcaaaagt gcgaatacca cttgcccgaa
    51361 aggcccgtcg cgagtacttt gtcgcgatat ttttgacatt ttcgactaca ggaatttttc
    51421 gatgccaaga gcgaacgaaa ttaaaaaagg tatggtactg aattacaacg gcaaactgct
    51481 gctggttaag gatattgata ttcagtcgcc cactgcccgc ggtgccgcta cgctgtacaa
    51541 aatgcgtttt tctgatgtcc gtaccgggct gaaagtagaa gagcgtttca aaggtgatga
    51601 tatcgttgac accgtgacgc tgacccgccg ttacgttgac ttctcctatg tcgatggcaa
    51661 cgaatatgtc tttatggata aagaagacta taccccgtat accttcacca aagatcagat
    51721 tgaagaagag ttgctgttta tgccagaggg cggcatgccg gacatgcagg tgctgacctg
    51781 ggacggtcaa ctgctggcgc ttgagctgcc gcagaccgtt gatctggaaa tcgttgaaac
    51841 cgcaccaggc atcaaagggg catctgccag cgcccgtaac aaaccggcga cattgagcac
    51901 tggtctggtg attcaggtac cagaatactt aagcccgggc gaaaaaattc gtatccatat
    51961 cgaagaacgc cgttatatgg ggcgtgctga ctaacttcag ccgcatgcag aaaagggata
    52021 gctcaggctg tccctttttt aatttattat accaatcttc tattttgcgc ttcctgaaca
    52081 ccttacgccc tcaattttca ctcgttgatt gatcgccctc acacttcatc gcattaacaa
    52141 tccagaccaa tttcaattgc tgtcatataa ctttacactg tcattgttaa tttatcgtta
    52201 ctaagacgtg actcctatga agacaattgc ctccgttacg ctcccgcatc atgtacacgc
    52261 tccacgctac gatcgccagc agttgcaatc acgtatcgtt cattttggct ttggcgcctt
    52321 tcaccgcgct catcaggcgt tactgaccga tcgtgtgctg aatgcccagg gcggcgactg
    52381 ggggatctgt gaaatcagct tgttcagcgg tgatcaactg atgagccagc tccgcgcaca
    52441 gaaccattta tataccgtgc tggagaaagg tgcggacggc aatcaggtga taattgtcgg
    52501 tgccgttcac gaatgcctta atgcaaaact ggattcctta gcggcaatta ttgagaaatt
    52561 ttgcgagcca caggtggcaa ttgtttccct gacgattacc gaaaaaggct attgtattga
    52621 cccggccacc ggtgcactcg acaccagtaa tccgcggatt attcacgatc tacaaacccc
    52681 tgaagaacct cactccgcac cgggtattct cgtcgaagca ctgaaacgcc gccgtgagcg
    52741 cggccttaca ccgtttaccg tgctctcctg cgacaatatt cccgacaatg gccatgtggt
    52801 gaaaaacgcg gtgctgggaa tggcagaaaa atgttcgcca gaactcgccg ggtggataaa
    52861 aaaacacgtc agttttccgg gaaccatggt cgaccgcatt gttccggctg caaccgacga
    52921 atcactggca gaaatcagcc agcatctggg ggtaaatgat ccctgcgcga ttagctgcga
    52981 accgtttatc cagtgggtgg ttgaagataa cttcatcgct gggcgtcctg cctgggaagt
    53041 cgcaggtgta caaatggtga atgatgtcct gccatgggaa gagatgaaac tgcgtatgct
    53101 taatggcagc cactcttttc tcgcttatct gggttacctc tcaggattcg tccatatcag
    53161 tgattgcatg caggatcgcg tatttcgcca tgctgccaga acattaatgc tggatgagca
    53221 agcgccgaca ctgcaaatta aagatgtcga tttaacacaa tatgcggata agttaattgc
    53281 acgttttgct aatccgtcgc tgaaacataa gacctggcaa atcgcgatgg atggcagcca
    53341 gaaattaccg caacgtatgc tggcaggtat tcgcatacat ctggggcgtg aaacggactg
    53401 gtcgttgctg gcattaggcg ttgctggctg gatgcgttac gtcagcggcg ttgatgatgc
    53461 cggaaatgcc attgatgttc gcgatccgct tagcgataaa attcgcgaac ttgttgcggg
    53521 cagcagcagt gaacaacgcg taaccgccct gctttccctg cgtgaagttt tcggtgatga
    53581 tctgccagat aacccgcatt ttgtgcaggc catcgaacaa gcctggcaac aaatcgtaca
    53641 attcggcgca catcaggcgc tattaaacac cctcaaaatt taacgatttc tgcggttaaa
    53701 gcggatgaag ctcaccttcg tccgctctcc ccttctcttt tctgcctttt ttagccagga
    53761 ttaacgctca gttaacttac cagagtattg cggagccgtt gtgaccagga ccaacctcat
    53821 caccggtttt ctcggcagcg ggaaaaccac gtcgattctt catctgttag cccataaaga
    53881 tcccaacgaa aaatgggcgg tactggttaa tgaatttggg gaagtcggaa ttgatggtgc
    53941 tttgctcgcc gatagcggcg cattgctgaa agagatcccc ggcggctgca tgtgctgcgt
    54001 taatggttta cccatgcagg tagggttgaa taccttactg cgtcagggaa aaccagaccg
    54061 cttgttgata gagccgaccg ggctgggcca tccgaaacag atcctcgatc tgttaaccgc
    54121 accagtctat gaactgtgga tagatctgcg cgccaccttg tgcattctcg atccacgcct
    54181 gctgctggac gaaaaaagcg ccagcaatga aaacttccgt gaccagctgg ctgccgcaga
    54241 catcattgtc gccaataaat ccgaccgtgc gacgcccgaa agtgagcaag cgctacagcg
    54301 ttggtggcag caaaatggtg gcgatcgaca attaattcac agtgagcatg ggaaagttga
    54361 cggtcatctt ctggatttgc cgcgtcgcaa tttagccgag ttgcccgcca gcgccgcgca
    54421 ttctcatcag catgtcgtga aaaaagggtt agcagcgtta agcctgccag agcatcaacg
    54481 ctggcgtcgc agtctgaaca gcgggcaagg atatcaggcc tgcggctgga tattcgacgc
    54541 tgatacggta ttcgacacca ttggcattct ggaatgggcg cgacttgcac cggtggaacg
    54601 cgtcaaaggc gtgctgcgta ttcccgaagg gctggtacga atcaaccgtc agggcgatga
    54661 cctgcacatt gaaacgcaaa acgttgcgcc accggacagc cgtattgagc tgatttccag
    54721 cagcgaagct gactggaatg ctttgcagag cgcgctgttg aagcttcgtt tagcgactac
    54781 cgcgtaaggt tgcctgcgtt tttcagtaag ataattagag aaaatatgat taaaaatttg
    54841 ccgcaaatag tgttgttgaa tattgtcggc ctcgcgctgt ttctttcctg gtatattccc
    54901 gttaatcatg gattctggtt gccgattgat gcggatattt tttatttctt taatcagaaa
    54961 ctggtcgaaa gtaaggcctt tttgtggctg gttgcattga ccaacaatcg cgctttcgac
    55021 ggttgttctc tgctggcgat gggaatgttg atgctgagtt tctggctgaa agaaaacgcc
    55081 cctggcagac gacgtatcgt gattatgggt ctggtcatgc tattaactgc agtggtatta
    55141 aaccagctgg gtcaggcatt aattcctgta aaacgggcca gcccaacatt gacttttacc
    55201 gatattaacc gcgtcagcga actgctctct gttcccacga aagatgcctc acgagatagc
    55261 tttcccggcg atcacggcat gatgctgctt attttttcgg cattcatgtg gcgttatttc
    55321 ggcaaagttg caggccttat cgcccttatt atttttgtgg tttttgcatt tcccagagta
    55381 atgattggcg cacactggtt tactgacatc attgtcggtt cgatgaccgt gatattgatc
    55441 ggtttgccct gggtgttgct gacgccatta agtgatcgat taatcacctt ttttgacaaa
    55501 tcactaccgg gaaaaaacaa acatttccaa aacaaataac tcacagtaat taacatcatc
    55561 aggcttattt ttatagtgag gataatcctg atgatgcgca ccgtgctttc atccatcgcg
    55621 cgcaaaaatc attctctaag taaatgaatg gattgcatgc gtttcactca atcgtacttt
    55681 aattgaccaa tcccgcttat taactttctg tatcactttt tcttataaaa aatcatgtaa
    55741 aaccgctcgc caagaccgca ccaatcgggt aatctagaac tcgttttgcc tcggcggtag
    55801 attatcctca cagcatataa ttttgtgcgt tagtccacag atttggcctt aaggaattgt
    55861 ttcaacatgc ccaggtaatt agtctcgtgt cgcttggcat ttttttataa cgatatttgt
    55921 cgttaaggac ttcaagggaa aacaaacaac atggtcaaat ctcaaccgat tttgagatat
    55981 atcttgcgcg ggatccccgc gattgcagta gcggttctgc tttctgcatg tagtgcaaat
    56041 aacaccgcaa agaatatgca tcctgagaca cgtgcagtgg gtagtgaaac atcatcactg
    56101 caagcttctc aggatgaatt tgaaaacctg gttcgtaatg tcgacgtaaa atcgcgaatt
    56161 atggatcagt atgctgactg gaaaggcgta cgttatcgtc tgggcggcag cactaaaaaa
    56221 ggtatcgatt gttctggttt tgtacaacgt acattccgtg agcaatttgg cttagaactt
    56281 ccgcgttcga cttacgaaca gcaggaaatg ggtaaatctg tttcccgcag taatttgcgt
    56341 acgggtgatt tagttctgtt ccgtgccggt tcaacgggac gccatgtcgg tatttatatc
    56401 ggcaacaatc agtttgtcca tgcttccacc agcagtggtg ttattatttc cagcatgaat
    56461 gaaccgtact ggaagaagcg ttacaacgaa gcacgccggg ttctcagccg cagctaataa
    56521 accgtttgga tgcaatccct tggctatcct gacgagttaa ctgaaagcac tgcttaggca
    56581 gtgctttttt gttttcattc atcagagaaa atgatgtttc cgcgtcttga tccaggctat
    56641 agtccggtca ttgttatctt ttaaatgttg tcgtaatttc aggaaattaa cggaatcatg
    56701 ttcatacgcg ctcccaattc tggacgtaag ctcctgctta cctgcattgt agcaggcgtg
    56761 atgattgcga tactggttag ctgccttcag tttttagtgg cctggcataa gcacgaagtc
    56821 aaatacgaca cactgattat cgacgtacaa aagtatctcg atacctattt tgccgacctg
    56881 aaatccacta ctgaccggct tcagccgctg accttagata cctgccagca ggctaacccc
    56941 gaactgaccg ctcgcgcggc gtttagcatg aatgtccgta cgtttgtgct ggtgaaagat
    57001 aaaaaaacat tctgttcatc tgcgactggt gagatggaca ttccactcaa tgaattgatt
    57061 ccggcgctca acattaataa aaacgtcgat atggcgatct tacccggcac gccgatggtg
    57121 ccgaacaaac ccgcaatcgt catctggtat cgcaaccctt tgctgaaaaa tagcggtgtg
    57181 tttgccgctc tgaatctcaa cctgacgcct tctctctttt atagttcacg gcaggaagat
    57241 tacgatggcg tcgccctcat tattggcaat actgcgctat ctaccttttc ttcacgtttg
    57301 atgaacgtta atgaattaac cgacatgccg gtccgtgaaa ctaaaattgc gggcattcct
    57361 ctgaccgttc ggctttatgc ggatgactgg acatggaacg atgtgtggta cgcattttta
    57421 ctgggcggca tgagtggaac tgtcgttggc ctgctctgct attacctgat gagcgtacgt
    57481 atgcgccccg gcagagaaat catgaccgcc atcaagcgcg aacaatttta cgtggcgtat
    57541 caaccggtgg tggatacaca agctttgcga gtaacgggcc tggaagtact gctacgctgg
    57601 cggcatcctg tcgcgggaga aattcccccg gatgccttca ttaactttgc cgaatcgcaa
    57661 aagatgattg tgccgctgac tcagcacctg tttgagttaa ttgcccgcga tgccgcagaa
    57721 ttagaaaaag tgctgccagt aggcgtcaaa tttggtatta acattgcgcc ggaccatctg
    57781 cacagcgaaa gctttaaagc agatatccag aaactgctca cttccctgcc cgcacaccat
    57841 ttccagattg tgctggaaat taccgagcgc gatatgctga aagagcaaga agccacacaa
    57901 ctcttcgcct ggctgcactc ggtcggcgta gaaattgcta ttgatgactt cggcaccggg
    57961 cacagcgcgc ttatctatct tgagcgtttt acgctcgatt atctgaaaat tgaccgtgga
    58021 tttatcaacg ccatcggtac ggaaacgatc acttcacccg tacttgacgc ggtgctgacg
    58081 ctggcgaaac gcctcaatat gctgacggtt gctgaagggg tcgaaacgcc ggaacaggcg
    58141 cgatggctaa gcgaacgcgg cgttaatttc atgcaaggct actggattag tcgcccttta
    58201 ccgctggacg attttgtccg ctggctgaag aaaccgtata cgccgcagtg gtaaggtgtg
    58261 cttacgtccc ttattattca tagtgaaagc atgccggatt gcggctaatg atgagtaaaa
    58321 ggaaatccat tgcagatgat tgtgcgcata ctgctgctgt ttatcgctct gttcaccttt
    58381 ggtgtgcagg cgcaggctat caaggaaagc tatgcctttg ccgtgctggg cgaaccccgg
    58441 tacgcgttta atttcaacca ttttgattat gtgaaccccg ccgcgccaaa aggtgggcag
    58501 ataacgttgt cagccctcgg caccttcgat aatttcaacc gctatgcact gcgcggcaac
    58561 ccgggcgcac gcaccgagca gctgtacgac acgctattta cgacttccga tgacgaacca
    58621 ggcagttatt acccgctgat tgctgaaagc gcacgctatg ctgacgatta ttcctgggtg
    58681 gaggtcgcta ttaatccgcg cgcccgtttt catgatggtt cgcccattac tgcccgcgat
    58741 gtagagttta cttttcaaaa atttatgacc gaaggcgtgc cgcaatttcg tctggtctac
    58801 aaaggcacca ccgtcaaagc cattgcaccg ttaaccgtgc gcattgagtt agctaaaccc
    58861 agcaaagaag atatgctgag tctgttttcg ctgccgatat ttccagaaaa gtactggaag
    58921 gatcacaaac ttagcgaccc gctcgccacg cctccgcttg ccagtggtcc gtaccgcgtt
    58981 acgtcctgga aaatggggca aaatattgtc tattcccgcg tgaaagatta ctgggcagca
    59041 aacttaccgg taaaccgtgg acgctggaat ttcgacacca ttcgctacga ttattacctc
    59101 gatgataatg tcgcctttga agcgtttaaa gcaggtgcct ttgatttgcg tatggaaaac
    59161 gacgctaaaa actgggccac gcgttatacc ggtaaaaatt tcgataaaaa atacatcatc
    59221 aaagatgagc aaaagaacga atcagcccag gatacgcgct ggctggcgtt taatatccaa
    59281 cgtccggtat tcagcgatcg ccgggtccgg gaagcaatca ctctcgcctt tgactttgaa
    59341 tggatgaaca aggcgttgtt ttacaacgcc tggagtcgca cgaacagtta ttttcagaat
    59401 accgaatacg cggccagaaa ttaccccgac gccgcggagc tggtgcttct ggcaccaatg
    59461 aaaaaagatc taccgccaga agtcttcaca caaatctacc agccgccggt atctaaaggc
    59521 gatggctacg atcgtgacaa cctgttaaaa gccgacaaac ttctcaacga aacgggctgg
    59581 gtgctgaagg gtcagcaacg cgttaatgcc acaacgggtc agccgctcag ctttgaatta
    59641 ttgcttcccg caagcagcaa tagtcagtgg gtattgccgt tccagcacag cctgcaacgg
    59701 ctgggtatca atatggacat tcgcaaggtg gataactcgc aaatcactaa ccgcatgcgc
    59761 agtcgcgact atgacatgat gccgcgccta tggcgggcga tgccgtggcc cagttccgat
    59821 ttacagattt cctggtcatc ggaatatatc aattccactt ataatgcccc cggcgtgcaa
    59881 agcccggtta tcgactcgct gatcaaccaa attattgccg cgcagggaaa taaagaaaaa
    59941 ttactgccgt tggggcgagc actggatcgc gtattaacgt ggaattatta catgctgcca
    60001 atgtggtaca tggcggaaga ccgtctcgcc tggtgggata aattctccca acccgctgta
    60061 cgccctgttt acagcctggg tatcgatacc tggtggtatg acgttaataa agcggccaaa
    60121 ctgccgtcag ccagacaaca gggagagtag atgggcgctt acctgattcg ccgtctgttg
    60181 ctggtgatcc caacgttatg ggctattatc actatcaact ttttcatcgt gcaaattgcg
    60241 cctggcggtc cggtcgatca ggccatcgcc gccattgagt ttggtaatgc cggagtatta
    60301 cccggcgcag gcggtgaagg tgttcgtgcc agccatgcgc aaacgggtgt cggcaatatc
    60361 agcgacagta attaccgtgg cggacgcgga ttagacccag aagtgatcgc tgagatcact
    60421 catcgctacg gtttcgataa gccgatccac gaacgttact tcaaaatgct ctgggactac
    60481 atccgctttg attttggtga tagcctgttt cgcagcgcct cggtgctgac gctgattaaa
    60541 gacagtctgc cggtttccat caccctcgga ttgtggagca cgctgattat ctatctggtg
    60601 tcgattccgt taggcattcg caaagctgtt tataatggga gtcgctttga cgtctggagt
    60661 agcgcattta tcatcatcgg ctacgccatt ccggcctttt tgtttgccat cctgctgatt
    60721 gtcttcttcg cgggcggcag ctatttcgac ctgttccctc tacgcggcct ggtttccgct
    60781 aactttgatt cgctgccgtg gtatcagaaa atcaccgatt atctgtggca tatcacgcta
    60841 ccggtgctgg cgacagtgat tggtggcttt gcggcgctga ccatgctgac caaaaactca
    60901 ttccttgatg aagtgcgcaa gcaatacgtg gtgaccgcgc gtgcgaaagg ggtaagtgag
    60961 aaaaacattc tctggaaaca tgtgttccgc aacgccatgc tgctggtgat tgccggtttt
    61021 ccggcgacgt ttatcagcat gttttttacc ggctcgctgc tgattgaggt gatgttttca
    61081 ctcaatggtc ttggcttact gggctacgaa gcgaccgtct cgcgcgatta tcctgtaatg
    61141 tttggtacct tgtatatttt caccctgatt ggcctgctgc tgaatattgt cagtgatatc
    61201 agctatacgc tggttgatcc gcgtatagat tttgagggac gttaatgtcg cgactcagcc
    61261 ccgtcaatca ggcccgttgg gcgcgttttc gtcataaccg tcgcggctac tggtcgttat
    61321 ggattttcct cgttttgttt ggtttgagtt tgtgttctga acttatcgct aacgataaac
    61381 cgttgctggt gcgttatgac ggcagttggt atttcccgtt gttgaaaaac tacagcgaaa
    61441 gcgattttgg cggcccgctg gcaagtcagg ctgattatca ggacccgtgg ctgaaacaac
    61501 ggctggaaaa taacggctgg gtactgtggg caccgattcg ctttggtgct accagtatca
    61561 actttgctac cgataagccc ttcccttctc caccctcccg gcaaaactgg ctgggaacgg
    61621 atgccaacgg cggcgatgtg ctggcacgca ttctctatgg cacgcggatc tcggttctgt
    61681 ttggcctgat gctgactctc tgttccagcg tgatgggcgt gctggcgggg gcgctacaag
    61741 gctattacgg cggtaaagtc gatctctggg gacaacgctt tattgaagta tggtcgggga
    61801 tgccgacgct gtttttgatt attttacttt ccagcgtcgt acagcctaac ttctggtggc
    61861 tgctggcaat tactgtcttg tttggctgga tgagtctggt cggcgtggtg cgggcggagt
    61921 ttttacgtac tcgtaatttc gactacattc gtgcggcaca ggcgcttggc gtcagcgatc
    61981 gcagtatcat cctgcgtcat atgttgccta atgccatggt cgcgaccctc acctttttac
    62041 cgtttatttt atgtagttcg ataaccaccc tgacctcgct cgatttcctc ggcttcggtc
    62101 taccgctcgg ttcaccgtca ctcggtgaac tgctgttaca agggaaaaat aaccttcagg
    62161 ccccgtggct tgggatcacc gccttcttgt cggtggcgat attattgtct ttgctgatct
    62221 ttattggtga agccgtccgc gacgcatttg atcctaataa ggcggtgtag catgacgcaa
    62281 actctgttag cgattgaaaa tttgtcggtg ggttttcgcc atcagcaaac cgtacgtaca
    62341 gtagtcaatg atgtttcact acagattgag gctggcgaaa cgctggcgct ggtgggtgag
    62401 tcaggttcag gcaaaagcgt taccgcgctg tcaattttac gcctgctccc ttccccgccg
    62461 gttgaatatc tctccggcga tattcgtttt catggcgaat cgctgcttca tgccagcgat
    62521 caaacgttgc gcggtgtacg cggtaataag atcgccatga tttttcagga accgatggtg
    62581 tcgttaaatc cattgcatac cctggaaaaa cagctttatg aagtgctttc actccaccgc
    62641 gggatgcgtc gggaagcggc tcgtggcgaa attcttaact gccttgatcg cgtcggtatc
    62701 cgccaggcgg caaaacggct gacagattat ccgcatcagc tctccggcgg cgaacgccag
    62761 cgggtgatga ttgcgatggc gctgttaacg cgaccggaat tattaattgc cgatgaaccg
    62821 accaccgcac tggacgtctc tgtccaggcg cagattttac agctgttgcg cgaactgcaa
    62881 ggcgagctga atatgggcat gctgtttatt actcataacc tcagcattgt cagaaaactg
    62941 gcccaccgcg tggcggtaat gcaaaacggt cgctgtgtcg agcaaaataa cgccgctacg
    63001 ctatttgcct cacccactca tccttacaca caaaagctac tcaacagtga accgtcaggc
    63061 gatccggtac cgttgccaga acctgcctca acgttgctgg atgttgaaca gcttcaggtt
    63121 gccttcccca ttcgcaaagg gattttgaag cgcattgtgg atcataatgt ggtggtgaaa
    63181 aacatcagtt ttacgctacg ggcgggtgaa acactgggtt tagtgggcga gtctggttcc
    63241 gggaaaagca cgacgggact ggcgctgctg cgactgatta cctctcaggg cagcatcgtc
    63301 tttgacggtc agccactgca agatttaaat cgccgccagc tgttacctac tcgtcatcgt
    63361 attcaggtgg tatttcagga tccaaactcc tcgctcaacc cacgactcaa cgttttgcag
    63421 attattgagg aaggcttacg ggttcaccag ccgacgcttt ctgccgcaca acgcgaacaa
    63481 caagtgatag ccgtgatgca tgaagtggga ttagatcctg aaacacgcca ccgttatccg
    63541 gcggagttct ctggtggtca gcgacaacgt attgcgattg ccagggcatt aattcttaag
    63601 ccctcactga tcatacttga tgaaccgaca tcatcactcg acaaaacggt acaggcgcaa
    63661 atattgacgc tattgaaatc attgcaacaa aagcatcaac tggcctattt gtttatcagc
    63721 cacgatttgc acgttgtccg cgcgttatgt catcaggtta tcgtactgcg acaaggggaa
    63781 gtagtggaac aaggaccgtg cgcgcgcgtg tttgccgcac cgcagcagga gtatacgcgt
    63841 cagctactgg cgttgagctg acgcttaaaa aggattgtag tttgaaaagg gttcggcaat
    63901 tgccacacca aaatttttca atcggcatgt tgcagcaaac tcgtcctggc gatttacaaa
    63961 caggcacggc tcaccttcac actccagcac cgaacatgga acttcgatgt cgcttaacgc
    64021 ctggcttagt ttatacatca ccgaccatgc attatcacca tccgggctaa gcaatttaag
    64081 cgccaccagg ctgttgtcac cgcacggtcc attttgcgga atcggttcaa ccttcgaacc
    64141 tgcgaaacca gcagaccagc gaaagttctg cggtagacga tggacgattg agagttgtaa
    64201 tgaagtcact tattttcccc ggaagcacat ttacttcaca atttatttac atcaatttta
    64261 acacatcatc aacaatccgt ctttatacag atcgtaaaga ttgatgcctc gcctgcatgg
    64321 cctctatggc tctgatttaa gtaatatctg cgggcgtggt cacgtttgcg gggctatatg
    64381 tacccgtttc tgcgatctaa ctcaaccttt ttaactacaa tgatgtgact ttttacataa
    64441 attgatttta cataaaataa acatatatcg ggggaatgat agatttgtgg ttgacatgca
    64501 tcaacaaagg aagcctttta gcttcctcgt tgtgcaatag atcaccgttt tttcggtcga
    64561 ctggcgtaca gacagaagag aatggagctg gttgcgcaga atgcaattga ccaaatcatc
    64621 ggccatgcag agttaaaggt cgcaagagaa agcaatgcgc caacaattgc ccctatgcca
    64681 aaacggaagg ttcctgccag cgaagatgcc gttcccgcca tatggggaaa ctcatcaaga
    64741 atgaccgcca tcgcattgga tgacaccata gacacgcagc ccacaaacgc cgcaacgcca
    64801 accaccagcg accagaatcc cagccccagc agcgcactga tgaccatcca cgctgccata
    64861 ataaattgta tccacaaccc cgagcggaac atatttaacg cgccaatgcg gcggacgaag
    64921 cggctgttaa agatggtcat cacgaacaga aaaacaatgt ttagcgcaaa gtaataacca
    64981 aagttttccg gtgcaacgtg gttgatttca atgtagacaa acggtccggc gctcaggaac
    65041 gagaacattc cggcaaagct gaaaccgctg gccagcatat aactcaggac acgcttatga
    65101 cggaacagcg ccgcaaagtt accaatagtg gtacgaatgt gaaaaggctg gcgaggatcc
    65161 agaagatgta atgccagctc agccacacca gcacccagcc gccaactatc ggtgccatca
    65221 gcggtgcaat ggtcgtcacc agcatgacaa acgacatcat ccgcgagaac tcttctttcg
    65281 ggtaaatatc acgcatcaaa gcgttgataa ccacgctggc cgcagccgca gccagcccgt
    65341 ggaagaaacg catcacaatc agctgatcga tggtttgtgc caacgcacac gccaccgcgg
    65401 cggcggcaaa caccagcgta ccgccaagaa ccaccggctt acgcccgaag ctgtctgcca
    65461 tcggcccgta gattaactgc cccaacgcaa agcccagaat ataagtactc agggtcatct
    65521 gcgtactgcc cgccggtacg ccaaactgcg ctgaaattac cggtagcgcg ggcagataca
    65581 tatcaatcga cagcggcatc aacatggcca gcaggccaag gataaaaaca atagcaaacg
    65641 acgaatgctg tcgggtggtc acaacgggct cctgaaagtc attgaaaagt tagacgacgc
    65701 tggcaatttc ttcttcagtt aacggacgat attcaccggg ggctaaatca gcatccagcg
    65761 taatgccgcc aatacgttca cgatgcagct caaccacatg gttacccacg gcggcgaaca
    65821 tgcgtttcac ctgatgataa cgcccttcgc tgatggtcag acgaacctgc gttggggtaa
    65881 tcacttccag caccgccggc ttagtgagat ctttttcgtt atgcagctgc acgcctttag
    65941 caaattgttc tgccgtatcg tcagctacgg gtgataccag cgtcaccaga taggtcttct
    66001 cgcaatgatg gcgcggagaa gtaatgcggt gcgaccactg accatcatct gtcattagta
    66061 ccagaccggt ggtatcgata tccagccgcc ctgcggcgtg cagtttccac gctaccggtt
    66121 catctagaaa atagagcacc gttgggtgat cagggtcgtc cgtggagcaa acataaccct
    66181 gaggcttatt gagcatgaag taacgtggac cgtgttgctg cgccagcggg ttgccatcgt
    66241 aagcgacatc atgttcagga agcagtttga acgctgcatt acggacgatt tcgccatcga
    66301 cggtgacacg attgccgcgg atttcacgcc cggcaatagc acggctaacg ccgagttgct
    66361 gtgcgataaa tttatcaagt cgcatgtgtg tgattttgcc tgtaaaaaac ggaggtcggg
    66421 taatgacccg aatatctgaa ctgttgtctg cccagtatag cggtctaata acatccctca
    66481 agggaaaaag atccgtggca tactattagc agaataatct acctacgcga gaccatgatt
    66541 tttacacttc gcccatatca gcaagaagcc gtggatgcca cgctcaacca ttttcgtcgt
    66601 cataaaaccc ctgccgttat cgtgctgccc accggtgcag gtaaaagcct ggtgatagcg
    66661 gaactggcac ggctggctcg tggtcgcgta ctggtgctgg cacacgttaa agaactggtg
    66721 gcgcaaaacc atgcaaagta tcaggcgctg gggctggaag ccgatatttt tgccgccggg
    66781 ctgaagcgta aagagagcca cggtaaagtg gtatttggca gtgtgcagtc ggtcgcccgt
    66841 aatattgatg cctttcaggg tgaattttcg ctgttgattg tcgatgaatg tcaccgtatt
    66901 ggtgacgatg aagagagcca gtatcagcaa atcctcactc acctgacaaa agtgaatccc
    66961 cacttacgcc tgctggggct gactgccacg ccttttcgat tgggcaaagg ttggatctac
    67021 cagttccatt atcacggcat ggtacgcagc gatgagaaag cccttttccg tgactgcatt
    67081 tatgagctgc ccctgcgtta tatgattaaa cacagctatc tgacgccgcc agaacgactg
    67141 gatatgccag tagtgcaata tgatttcagc cgcttgcagg cacagagcaa cgggctgttc
    67201 agcgaagccg atctcaaccg tgagctgaaa aaacagcaac gtattacccc gcacatcatc
    67261 agccagatta tggagttcgc cgaaaagcgt aaaggagtga tgattttcgc cgcgacggtc
    67321 gaacacgcaa aagagattgt gggattactg cctgccgaag atgcagcact gattactggc
    67381 gacacccccg gcgctgagcg cgatgtgtta attgaaaatt ttaaagccca gcgttttcgc
    67441 tatctggtca acgtcgcggt actgaccacc ggatttgacg ccccgcacgt cgatcttatc
    67501 gccattctgc gccctaccga atcagtgagt ctttaccaac aaattgtcgg gcgcggtctg
    67561 cgtctcgctc cgggcaagac tgattgttta attcttgatt atgcgggtaa tcctcacgat
    67621 ctctacgcgc cggaagttgg tacaccgaaa ggcaaaagtg acaacgttcc ggtacaggtt
    67681 ttctgccctg cctgcggttt tgccaacacc ttttggggga aaacgaccgc cgacgggaca
    67741 ttgattgaac actttggtcg ccgctgtcag ggatggtttg aagatgacga cggtcatcgc
    67801 gaacagtgtg acttccgttt ccgttttaaa aattgcccgc aatgtaacgc agaaaatgat
    67861 attgccgctc gccgctgccg cgaatgtgac acggtactgg ttgatccgga tgatatgtta
    67921 aaagcggcgc tacgactgaa agatgcgctg gtattacgct gtagcggcat gtctttgcag
    67981 catgggcacg acgagaaagg cgaatggttg aaaatcacct attacgatga agacggtgcg
    68041 gatgtgagtg agcgtttccg tctgcaaaca cctgcccagc gtaccgcctt cgagcagctt
    68101 tttatccgcc cgcatacgcg cacaccgggc atcccgctgc gctggatcac cgccgccgat
    68161 atcctcgccc agcaagcctt attgcgaccc ccggattttg tcgtcgcccg catgaaaggc
    68221 cagtactggc aggtgcgtga aaaagtgttc gattacgaag gtcgttttcg tcgggcgcac
    68281 gaattacgcg gttaatggta gttttgattg atgtataagg cgattgagta tagaatctca
    68341 cccgcttttg catacgcaaa gcagatcact tacctgttgc tgggtcgcct gtagcaggat
    68401 taatttaaga gagaaagaaa tgtttactat caacgcagaa gtacgtaaag agcagggtaa
    68461 gggtgcgagc cgccgcctgc gtgccgctaa caagttcccg gcaatcatct acggtggcaa
    68521 agaagcgccg ctggctatcg agctggatca cgacaaagtc atgaacatgc aagctaaagc
    68581 tgaattctac agcgaagttc tgaccatcgt tgttgacggt aaagaaatca aagttaaagc
    68641 tcaggacgta cagcgtcacc cgtacaaacc gaagctgcag cacatcgact tcgttcgcgc
    68701 ttaattgctg aataagttgt aaaaaacccc gctcccgcgg ggttttttgt atctgcagat
    68761 tatgcctgat tacggtattg ctattttttg caggccagat aaggcgttca cgccgcatct
    68821 ggcattcaga acacaaaacc gattaattgc cgccagacgt ccggcgctgc aattggtcgc
    68881 gcaaattcgg cggcgtgcct ttaatggtca gggtatcggt cgccggctcc cagaagatgc
    68941 gttcacctaa cagcatcgca tcaaagttaa tagtcaagcc accgccgcta ccggcaaact
    69001 tcgtcaactg acgcagtgtg ctccggtctg ccgggaagct ctcttccagt tcgtagcctt
    69061 tttcagccgc aaactccgtg aagctcactt cgcttacgcc tgccagctct ttcgacagcg
    69121 attccagttc gatctcttcg cctgcctgca gctgctcatt acagtagcta taaacctgct
    69181 ggcgcacgtt ctgtcgctct gctttatcca gttgtgcttc ggcagtgaaa tcatcaaccg
    69241 cttgcaacag cccacggttt tgtgctttcg tgttcaatcc ttcgctggcc ccgaggaaat
    69301 ccataaagaa atccgccact ttgcgcccta ctcgcccttt caggaaagtg agatagcggg
    69361 tggactctgg attggtttcc cattcggtta aatcgatacg cgcaacaata tccgcatgat
    69421 tgatatcaag ataatgggtt gggttgatgt cgagattttc gttaacacgc atactgctca
    69481 ggttgctcag aactgccacc agcagatact ccaccgccag ataacggtag tggcaaaaca
    69541 gcacgaatcc accgtcagca aaaggatatt tcgctaattc gtcacgcagg cgaccagttg
    69601 ccgcgcggct aaatgccaga aagtcctctt cgccctgacg ctgtaagcgc agcgtctgcg
    69661 ccagttcact ctcttcgcta aacaagccgt aagctttatt tttggcgcta tagacccgat
    69721 gcagttctgc caccatctca acgacggttt ctgtcggttc cagcaatgaa tcgcgcaaca
    69781 ccagctcaag gttctgctca tcacgcttga taagctggtg cagagcaatc tggttgatat
    69841 ccagactcat gataaactct cctttaagac cgggcggtat tcaaccaccg ccggttaagc
    69901 gacgcaataa aagatggcgg tcattttaca gaaggcagcg taacgagaat gatttaacgc
    69961 ataaaaaagc agaaaaaaaa ccgttgctac ggtaatatgt tgccctttca tcaactaact
    70021 gatttcgatt tatgccacaa atttcccgct acagtgatga acaggttgaa caattgctcg
    70081 cagagctgct caacgtactg gaaaaacata aggctccgac cgacctttcc ctgatggtgt
    70141 taggcaacat ggccactaac cttatcaaca ccagcattgc cccggcccaa cgccaggcaa
    70201 ttgccaactc ttttgcccgc gccttacagt cctctatcaa cgaagacaaa gcgcactaag
    70261 ggaaacagat aacaggttat ggtaactcat cgtcagcgct accgtgaaaa agtctcccag
    70321 atggtcagtt gggggcactg gtttgcactg ttcaatattc tgctttcgct cgtcattggc
    70381 agccgttacc tgtttatcgc cgactggccg acaacgcttg ctggtcgcat ttattcctac
    70441 gtaagcatta tcggccattt cagcttcctg gtgttcgcca cctacttgct gatcctcttc
    70501 ccgctgacct ttatcgtcgg ctcccagagg ctgatgaggt ttttgtccgt cattctggca
    70561 acggcgggaa tgacgctatt actgatcgat agcgaagtct ttactcgttt ccatctccat
    70621 cttaatccca tcgtctggca gttggttatc aacccagacg aaaatgagat ggcgcgcgac
    70681 tggcagctga tgttcatcag cgtgccggtt attttattgc ttgaactggt gtttgcgacg
    70741 tggagctggc aaaagctgcg cagcctgacg cgtcgtcgac gcttcgcgcg cccgctggcc
    70801 gcattcttat ttatcgcctt tatcgcctcg catgtggtgt atatctgggc cgatgccaac
    70861 ttctatcgcc cgatcaccat gcagcgcgct aacctgccgc tttcgtaccc gatgacggcg
    70921 cgacgttttc ttgagaagca tggtctgctt gatgcgcagg agtatcaacg ccgtcttatt
    70981 gagcaaggta atccagacgc cgtttccgtt cagtatccgt taagcgaact gcgctatcgc
    71041 gatatgggca ccgggcagaa tgtgctgttg attactgtcg atggcctgaa ctactcacgc
    71101 ttcgagaagc agatgcctgc gctggcaggt tttgctgagc aaaatatttc gttcacgcgc
    71161 catatgagct ccggcaacac tacagacaac ggcatctttg gcctgttcta tggtatctcg
    71221 ccgagctata tggacggcat tctgtcgacc cgtacgcctg cggcattaat tactgcgctt
    71281 aatcagcaag gctatcagct ggggttattc tcatcagatg gctttaccag cccgctgtat
    71341 cgccaggcat tgttgtcaga tttctcgatg ccgagcgtac gcacccaatc cgacgagcag
    71401 accgccacgc agtggatcaa ctggctgggc cactacgcac aagaagataa ccgctggttc
    71461 tcgtgggttt ctttcaatgg cactaacatt gacgacagca atcagcaggc atttgcacgg
    71521 aaatatagcc gggcggcagg caatgtcgat gaccagatca accgcgtgct caatgcactg
    71581 cgtgattctg gcaaactgga caatacggtt gtgattatca ctgccggtcg gggtattccg
    71641 ctgagcgaag acgaagaaac ctttgactgg tcccacggtc atctgcaggt gccattagtg
    71701 attcactggc caggcacgcc ggcgcagcgt attaatgcgc tgactgatca taccgatctg
    71761 atgacgacgc tgatgcaacg cctgctacat gtcagcacac ctgccagcga atattcgcaa
    71821 ggtcaggatt tgttcaaccc tcaacgccgt cattactggg ttactgcagc ggataacgat
    71881 acgctggcaa ttaccacccc gaaaaagacg ctggtgctga acaataacgg taaataccgc
    71941 acttacaact tacgtggtga aagagtgaaa gatgaaaaac cacagttaag tttgttattg
    72001 caagtgctga cagacgagaa gcgttttatc gctaactgat taattataaa tcagttagcg
    72061 aaatatctta cttgcaatcg gtgtggaaaa cggtagtatt agcagccacg agtcggcacg
    72121 tagcgcagcc tggtagcgca ccgtcatggg gtgtcggggg tcggaggttc aaatcctctc
    72181 gtgccgacca aaaatcccaa gaaaaaacca acctttacgg ttggtttttt tatatctgca
    72241 attaatttga taaacagacc gtgacacatc acagcctgtt tattttctgt tatcagaacg
    72301 tccagaccac tcccgcctga gtattccacg gcgattctac ccctgcgcca ttgccatagc
    72361 tgactgacaa atgaccgctt aacgtcgggg taaacgatga ccttatccca gcctgataaa
    72421 cgccacgggt tccagccaca tcattattaa atttgccatc gtcattaact ttcacatggt
    72481 tagaatcggc gtattcctga cgcacggcgg ctttcagcca gggttccaac gtcgtaccgt
    72541 tttgcaggtc catgtgatag cttaccgccg ttcccgcttc agcgcgtaat atccgggtat
    72601 ttcccacatc cgcgcgcatg ccgtttgata acgtgtagtc ctgaccatct gtggtaaagc
    72661 cggtaaaggc gagatagggt ctaacactcc acaatccgtc aacccaacgg aacccgctct
    72721 caacatgagc acccgcgccg ttactattgt aatcgccaaa cgctgttgcc ccattactca
    72781 tcttgccatg gatggtgttg gcaaaacggt caactttcac caccccatca acataggcac
    72841 cgttctgatg ctcccaaccg gcataagccc ccagggtgta gctatcaata ttgcctttgc
    72901 cgccgcgatc aaaaccaata tcagaatgag agtaatcaaa gaacaagccg cgaattgtgc
    72961 tgctttcttc acgggagaaa cggctatcga taccgagcgt caggcccgtc aatgtttgct
    73021 caaagccagc tcccgcatca gtggtcacgt tgttgcgggt gttaattgcc gaactccaca
    73081 tcgccgtatc gtaattaacg ccttttacgc taccaagacg ctcacgcacg gtgtccagtt
    73141 ctgcatcaaa taccagcggt tgtgctgctg ccatgttcag cacatcagtg gttgaagggg
    73201 taatttgcgc gcgattctct gcgaggctcc agctatggtt gccattatcc agcaaggtat
    73261 attcatacgt accgatatca acaacgcctc cggcattgcc caacgtgaat gcagcatcac
    73321 cgccgcccgt cgttaccagt gtaaggctat ctcctgctgc cgggctggcc cccgtgtccg
    73381 tcacgaatat tttgaaatca cctgttgcct gaccgatgac gttgagctga tcgctctgat
    73441 gattagccat atcggtacgc atataaaatt gccgtttccc gacagggtat tggtggtcaa
    73501 cgtaatgaaa ttttccgcac ttgccgtcac agatgaacga gtcactggct cagccatcat
    73561 aatcacattt gcgccggaca tcgcaaggtt ggctaagttg aggcgagtgt tttggttcgt
    73621 cgtagtgata tcggcaatga tggtccatgc gctattttcg agcgaaacgt ccgcttggct
    73681 ggtatccacg gcaccatgcg ttctaaaaga agcgccctca gaaaccacta ccttacccac
    73741 caacatagcg ggtgcataat ttattttcgg cttcatgacc tcaagaatgc catcattccc
    73801 tctgactgac acgttaacca ttgtgccagc ccagacatta gcgcggccat tgttgataac
    73861 catattttca gagatggctt tagaggaata cgtgatactt ccattctgat ctgatcgacc
    73921 gagatcatat acagcatttg cctgcacttt cgtaccagta tcctttccca gcgattgcat
    73981 agtggcatgc tcatcaagga tagtgtcaat ggcctgagtg tcctccataa caatgagccc
    74041 ggagccatca tccagttcat aattttttga cacaccatcc tttatactaa attggccttt
    74101 actgtttgta ccactcactt ccagcgcatt cgttgagaca attaatttcc cgcctgcttt
    74161 tttatcaacg ccagttgcag tcccgccatc cataacctcc agtaatcctc ccttttcgat
    74221 agcggcaaag acggcaatcc catctttttc aacaattaag cgcccgcctt taatcgtggg
    74281 atcattcgcc gcg
//
LOCUS       DAEEKQ010000005        70301 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.005, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000005 DAEEKQ010000000
VERSION     DAEEKQ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70301
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.005"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..673)
                     /locus_tag="KLK57_000313"
     CDS             complement(<1..673)
                     /locus_tag="KLK57_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HBE0890797.1"
                     /translation="MRNKLSFDLQLSARKAAIAERITAHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRH"
     gene            complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="KLK57_000314"
     CDS             complement(695..2707)
                     /gene="hyfR"
                     /locus_tag="KLK57_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HBE0890798.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGCRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSHLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGLSVREVL"
     gene            complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="KLK57_000315"
     CDS             complement(2737..3150)
                     /gene="hyfJ"
                     /locus_tag="KLK57_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HBE0890799.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFPCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="KLK57_000316"
     CDS             complement(3143..3901)
                     /gene="hyfI"
                     /locus_tag="KLK57_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HBE0890800.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="KLK57_000317"
     CDS             complement(3898..4437)
                     /gene="hyfH"
                     /locus_tag="KLK57_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HBE0890801.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACPANALTIQTDDQQNSRTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTNKA
                     DLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPECKQ
                     RATLLNDDTDVPLVAKEQL"
     gene            complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="KLK57_000318"
     CDS             complement(4447..6162)
                     /gene="hyfG"
                     /locus_tag="KLK57_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HBE0890802.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDNWPEDMHPLRKDAMDYRL
                     RPEPTTDTETYPFINEGNSDARVIPIGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLATPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="KLK57_000319"
     CDS             complement(6152..7732)
                     /gene="hyfF"
                     /locus_tag="KLK57_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HBE0890803.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHILNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALAGMPPFNIFLSEFMTITAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKPPQ
                     AVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRSTW
                     HDFLPSGTASVSEKHSER"
     gene            complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="KLK57_000320"
     CDS             complement(7737..8387)
                     /gene="hyfE"
                     /locus_tag="KLK57_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HBE0890804.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="KLK57_000321"
     CDS             complement(8399..9751)
                     /gene="hyfD"
                     /locus_tag="KLK57_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HBE0890805.1"
                     /translation="MGVLFAALTTLCMLSLISAFYQADKVAVTLTLVNVGDVALFGLV
                     IDRMSTLILFVVVFLGLLVTIYSTGYLTDKNREHPHNGTNRYYAFLLVFIGAMAGLVL
                     SSTLLGQLLFFEITGGCSWALISYYQSDKAQLSALKALLITHIGSLGLYLAAATLFLQ
                     TGTFALSAMSELHGDARYLVYGGILFAAWGKSAQLPMQAWLPDAMEAPTPISAYLHAA
                     SMVKVGVYIFARAIIDGGNIPHVIGGVGMVMALVTILYGFLMYLPQQDMKRLLAWSTI
                     TQLGWMFFGLSLSIFGSRLALEGSIAYIVNHAFAKSLFFLVAGALSYSCGTRLLPRLR
                     GVLHTLPLPSVGFCVAALAITGVPPFNGFFSKFPLFAVGFALSVEYWILLPAMILLMI
                     ESVASFAWFIRWFGRVVPGKPSEAVADAAPLPGSMRLVLIVLIVMSLISSVIAATWLQ
                     "
     gene            complement(9727..9837)
                     /locus_tag="KLK57_000322"
     CDS             complement(9727..9837)
                     /locus_tag="KLK57_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HBE0890806.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="KLK57_000323"
     CDS             complement(9854..10801)
                     /gene="hyfC"
                     /locus_tag="KLK57_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HBE0890807.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEDAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="KLK57_000324"
     CDS             complement(10812..12830)
                     /gene="hyfB"
                     /locus_tag="KLK57_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
                     /protein_id="HBE0890808.1"
                     /translation="MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGV
                     IGIISGITQLHAGVTLVARFATPFEFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTY
                     MREYEGKGAAAMGFFMNIFIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTS
                     INAGMLYFFIAHAGSVLIMIAFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGF
                     GAKAGMMPLHSWLPRAHPAAPSHASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWG
                     ILVMAIGAISALLGVLYALAEQDIKRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLT
                     VVGLLGALFHLLNHALFKGLLFLGAGAIISRLHTHDMEKMGALAKRMPWTAAACLIGC
                     LAISAIPPLNGFISEWYTWQSLFSLSRVEAVALQLAGPIAMVMLAVTGGLAVMCFVKM
                     YGITFCGAPRSTHAEEAQEVPNTMIVAMLLLAALCVLIALSASWLAPKIMHIAHAFTN
                     TPPVTVASGIALVPGTFHTRVTPSLLLLLLLAMPLLPGLYWLWCRSRRAAFRRTGDAW
                     ACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQLDPTLRLNKGLAHVTARAQSTEPF
                     WDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVVAALVVLLIAIAV"
     gene            complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="KLK57_000325"
     CDS             complement(12830..13447)
                     /gene="hyfA"
                     /locus_tag="KLK57_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HBE0890809.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     LTAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAPAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQR"
     gene            complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="KLK57_000326"
     CDS             complement(13700..14170)
                     /gene="bcp"
                     /locus_tag="KLK57_000326"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HBE0890810.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(14170..14742)
                     /locus_tag="KLK57_000327"
     CDS             complement(14170..14742)
                     /locus_tag="KLK57_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HBE0890811.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            14888..15766
                     /gene="dapA"
                     /locus_tag="KLK57_000328"
     CDS             14888..15766
                     /gene="dapA"
                     /locus_tag="KLK57_000328"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HBE0890812.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            15783..16817
                     /gene="bamC"
                     /locus_tag="KLK57_000329"
     CDS             15783..16817
                     /gene="bamC"
                     /locus_tag="KLK57_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HBE0890813.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            17030..17743
                     /gene="purC"
                     /locus_tag="KLK57_000330"
     CDS             17030..17743
                     /gene="purC"
                     /locus_tag="KLK57_000330"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HBE0890814.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMLNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            17911..18759
                     /locus_tag="KLK57_000331"
     CDS             17911..18759
                     /locus_tag="KLK57_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HBE0890815.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHNMQ
                     QQGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGDPAQCN
                     TFGKSI"
     gene            18774..20789
                     /gene="tmcA"
                     /locus_tag="KLK57_000332"
     CDS             18774..20789
                     /gene="tmcA"
                     /locus_tag="KLK57_000332"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HBE0890816.1"
                     /translation="MAELTALHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSDPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGVGQQLIASALQYRPGLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGETLPVD
                     PLNNAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNTSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            20863..21561
                     /gene="ypfH"
                     /locus_tag="KLK57_000333"
     CDS             20863..21561
                     /gene="ypfH"
                     /locus_tag="KLK57_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE0890817.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIEMVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(21671..21871)
                     /locus_tag="KLK57_000334"
     CDS             complement(21671..21871)
                     /locus_tag="KLK57_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HBE0890818.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="KLK57_000335"
     CDS             complement(21899..23026)
                     /gene="dapE"
                     /locus_tag="KLK57_000335"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HBE0890819.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIDRLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(23030..23386)
                     /locus_tag="KLK57_000336"
     CDS             complement(23030..23386)
                     /locus_tag="KLK57_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HBE0890820.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            23768..23827
                     /gene="ypfM"
                     /locus_tag="KLK57_000337"
     CDS             23768..23827
                     /gene="ypfM"
                     /locus_tag="KLK57_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HBE0890821.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="KLK57_000338"
     CDS             complement(23926..27039)
                     /gene="acrD"
                     /locus_tag="KLK57_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HBE0890822.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGIGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENIERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVA
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="KLK57_000339"
     CDS             complement(27203..28903)
                     /gene="narQ"
                     /locus_tag="KLK57_000339"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HBE0890823.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSSVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            29109..31088
                     /gene="aegA"
                     /locus_tag="KLK57_000340"
     CDS             29109..31088
                     /gene="aegA"
                     /locus_tag="KLK57_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HBE0890824.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHLTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRLVPIEG
                     SEFVMPADAVIMVFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            31156..31731
                     /gene="nudK"
                     /locus_tag="KLK57_000341"
     CDS             31156..31731
                     /gene="nudK"
                     /locus_tag="KLK57_000341"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HBE0890825.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKTVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            31857..32900
                     /locus_tag="KLK57_000342"
     CDS             31857..32900
                     /locus_tag="KLK57_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HBE0890826.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAALAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="KLK57_000343"
     CDS             complement(32996..34999)
                     /gene="tkt"
                     /locus_tag="KLK57_000343"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBE0890827.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKDIYHAWDAREKGEKAQQRWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPLLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEITRGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(35019..35969)
                     /gene="tal"
                     /locus_tag="KLK57_000344"
     CDS             complement(35019..35969)
                     /gene="tal"
                     /locus_tag="KLK57_000344"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBE0890828.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQYAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            36258..38537
                     /gene="maeB"
                     /locus_tag="KLK57_000345"
     CDS             36258..38537
                     /gene="maeB"
                     /locus_tag="KLK57_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HBE0890829.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRERAPELMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            38830..39165
                     /gene="eutS"
                     /locus_tag="KLK57_000346"
     CDS             38830..39165
                     /gene="eutS"
                     /locus_tag="KLK57_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HBE0890830.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGVIGIM
                     TLTPGETAMIVGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            39178..39657
                     /gene="eutP"
                     /locus_tag="KLK57_000347"
     CDS             39178..39657
                     /gene="eutP"
                     /locus_tag="KLK57_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HBE0890831.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            39632..40333
                     /gene="eutQ"
                     /locus_tag="KLK57_000348"
     CDS             39632..40333
                     /gene="eutQ"
                     /locus_tag="KLK57_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HBE0890832.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEARKVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAQPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            40330..41133
                     /gene="eutT"
                     /locus_tag="KLK57_000349"
     CDS             40330..41133
                     /gene="eutT"
                     /locus_tag="KLK57_000349"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HBE0890833.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKPPLTVKQIQQRLGETQ"
     gene            41130..42146
                     /gene="pta"
                     /locus_tag="KLK57_000350"
     CDS             41130..42146
                     /gene="pta"
                     /locus_tag="KLK57_000350"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBE0890834.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRALTGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            42185..42478
                     /gene="eutM"
                     /locus_tag="KLK57_000351"
     CDS             42185..42478
                     /gene="eutM"
                     /locus_tag="KLK57_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HBE0890835.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            42585..42872
                     /gene="eutN"
                     /locus_tag="KLK57_000352"
     CDS             42585..42872
                     /gene="eutN"
                     /locus_tag="KLK57_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HBE0890836.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            42884..44287
                     /locus_tag="KLK57_000353"
     CDS             42884..44287
                     /locus_tag="KLK57_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HBE0890837.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPRIAGLGLGGEGWTTMTITTPTGEGVTSVR
                     TFVRLRRCVLVDAFRIV"
     gene            44298..45134
                     /gene="eutJ"
                     /locus_tag="KLK57_000354"
     CDS             44298..45134
                     /gene="eutJ"
                     /locus_tag="KLK57_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HBE0890838.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQSVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGEEIWPTVKPVYEKMVDIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            45124..46311
                     /gene="eutG"
                     /locus_tag="KLK57_000355"
     CDS             45124..46311
                     /gene="eutG"
                     /locus_tag="KLK57_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HBE0890839.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDRD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAALEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            46628..47854
                     /gene="eutH"
                     /locus_tag="KLK57_000356"
     CDS             46628..47854
                     /gene="eutH"
                     /locus_tag="KLK57_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HBE0890840.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATTAKTEAEAQS"
     gene            47851..49254
                     /gene="eutA"
                     /locus_tag="KLK57_000357"
     CDS             47851..49254
                     /gene="eutA"
                     /locus_tag="KLK57_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HBE0890841.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMVLSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATALHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALLQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            49266..50627
                     /gene="eutB"
                     /locus_tag="KLK57_000358"
                     /pseudo
     CDS             49266..50627
                     /gene="eutB"
                     /locus_tag="KLK57_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
     gene            50648..51535
                     /gene="eutC"
                     /locus_tag="KLK57_000359"
     CDS             50648..51535
                     /gene="eutC"
                     /locus_tag="KLK57_000359"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HBE0890842.1"
                     /translation="MDQKQIEEIVRSVMASMGQAAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRTGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            51545..52204
                     /gene="eutL"
                     /locus_tag="KLK57_000360"
     CDS             51545..52204
                     /gene="eutL"
                     /locus_tag="KLK57_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HBE0890843.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            52217..52717
                     /gene="eutK"
                     /locus_tag="KLK57_000361"
     CDS             52217..52717
                     /gene="eutK"
                     /locus_tag="KLK57_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311336.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HBE0890844.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            52763..53815
                     /gene="eutR"
                     /locus_tag="KLK57_000362"
     CDS             52763..53815
                     /gene="eutR"
                     /locus_tag="KLK57_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HBE0890845.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSELVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(53821..54720)
                     /gene="hemF"
                     /locus_tag="KLK57_000363"
     CDS             complement(53821..54720)
                     /gene="hemF"
                     /locus_tag="KLK57_000363"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HBE0890846.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(54724..55593)
                     /gene="amiA"
                     /locus_tag="KLK57_000364"
     CDS             complement(54724..55593)
                     /gene="amiA"
                     /locus_tag="KLK57_000364"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HBE0890847.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            55807..56232
                     /locus_tag="KLK57_000365"
     CDS             55807..56232
                     /locus_tag="KLK57_000365"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HBE0890848.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            56219..56668
                     /locus_tag="KLK57_000366"
     CDS             56219..56668
                     /locus_tag="KLK57_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HBE0890849.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            56729..57304
                     /locus_tag="KLK57_000367"
     CDS             56729..57304
                     /locus_tag="KLK57_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HBE0890850.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            57400..58299
                     /gene="yfeX"
                     /locus_tag="KLK57_000368"
     CDS             57400..58299
                     /gene="yfeX"
                     /locus_tag="KLK57_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HBE0890851.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(58357..59661)
                     /gene="pbp4b"
                     /locus_tag="KLK57_000369"
     CDS             complement(58357..59661)
                     /gene="pbp4b"
                     /locus_tag="KLK57_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HBE0890852.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQIDVGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(59666..59839)
                     /locus_tag="KLK57_000370"
     CDS             complement(59666..59839)
                     /locus_tag="KLK57_000370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylglucosamine transporter subunit
                     IIBC"
                     /protein_id="HBE0890853.1"
                     /translation="MGLNSAFGPSGLVALPLMTSAQGILPAMAVYAGGILVAWVCGFI
                     FTTLFGCRNVNLD"
     gene            complement(59852..61090)
                     /gene="murP"
                     /locus_tag="KLK57_000371"
                     /pseudo
     CDS             complement(59852..61090)
                     /gene="murP"
                     /locus_tag="KLK57_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040446.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
     gene            complement(61094..61990)
                     /gene="murQ"
                     /locus_tag="KLK57_000372"
     CDS             complement(61094..61990)
                     /gene="murQ"
                     /locus_tag="KLK57_000372"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HBE0890854.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGTEDSREGGINDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            62154..63011
                     /gene="murR"
                     /locus_tag="KLK57_000373"
     CDS             62154..63011
                     /gene="murR"
                     /locus_tag="KLK57_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HBE0890855.1"
                     /translation="MLYLTKIRNAESEFTENEQKIADFLRANVSELKSVSSRKMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVAYEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            63140..63931
                     /gene="ucpA"
                     /locus_tag="KLK57_000374"
     CDS             63140..63931
                     /gene="ucpA"
                     /locus_tag="KLK57_000374"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HBE0890856.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            64102..65118
                     /gene="cysP"
                     /locus_tag="KLK57_000375"
     CDS             64102..65118
                     /gene="cysP"
                     /locus_tag="KLK57_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HBE0890857.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWTKENGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQTRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            65118..65951
                     /gene="cysT"
                     /locus_tag="KLK57_000376"
     CDS             65118..65951
                     /gene="cysT"
                     /locus_tag="KLK57_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HBE0890858.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            65951..66826
                     /gene="cysW"
                     /locus_tag="KLK57_000377"
     CDS             65951..66826
                     /gene="cysW"
                     /locus_tag="KLK57_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HBE0890859.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            66816..67913
                     /gene="cysA"
                     /locus_tag="KLK57_000378"
     CDS             66816..67913
                     /gene="cysA"
                     /locus_tag="KLK57_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HBE0890860.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRDRSNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            68047..68958
                     /gene="cysM"
                     /locus_tag="KLK57_000379"
     CDS             68047..68958
                     /gene="cysM"
                     /locus_tag="KLK57_000379"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HBE0890861.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(68961..69329)
                     /locus_tag="KLK57_000380"
     CDS             complement(68961..69329)
                     /locus_tag="KLK57_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HBE0890862.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            69434..70285
                     /gene="pdxK"
                     /locus_tag="KLK57_000381"
                     /gene_synonym="thiJ"
     CDS             69434..70285
                     /gene="pdxK"
                     /locus_tag="KLK57_000381"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HBE0890863.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
BASE COUNT        16699 a        18347 c        19437 g        15818 t
ORIGIN      
        1 aatggcgaat ggcaatggcg aacggaatca caaacaaatt ggcgatacag tgctcaaacc
       61 cactggcgac aaacagggtg atgggcaaaa tcatggcgac gattttgtcg cataatgaac
      121 gcccgcaata actcatccac agcgccagac aaaccatcag attgcacata atgccgaggc
      181 tgacagattc agtaaatgta tgatgcattt tgccctcggc gcagtgtaaa accgcgactc
      241 cccacatcgc gttttcactc atcaccagcc cggaaaacca gattaacaaa ctgaaacagg
      301 caatacctgc cagattaccg caggccacca gaagtgcgtt aatcagccaa gttcgccaac
      361 taataacgcc ccgacttttt gccatcaccg tcattaccga cgaggtgaac aggctggtgc
      421 cacaaaccgc cagcaaaata aagccgagtg taaagcaaag gccgcccacc agatgggtta
      481 atgcctgtga agacggggca tcggcgataa cggaaaggta aaaagtaaat ccaatcgcca
      541 taaacacgcc agcggacatc gccattaaaa agaccgacac tttgctgcgg gcaattttat
      601 gggcagtaat ccgttcagcg attgccgctt ttctggcgct caactgcaag tcgaaagaga
      661 gtttgtttct catgcatact cctgcaaaag cagattacaa cacctcgcga accgagagcc
      721 ccagacgctg cattcgtgac agcagcgtgg tgcgcttcat ccctaatcgt gtcgccgcgc
      781 cacggggccc ggcaacaatg ccattggttt ctcgcaatac ctgaacaatg cgctgacgtt
      841 cttcttcgtc attctctggc gtcgtcgggt gcagcaactg cgccattgaa ctgcgaagcg
      901 ctgaatcttc atttagcgtc ggcagtaaat ggctttgtcg gacattaaga tgtaaattca
      961 gactgttacc gcgagtcagc agtaccgctc gctcaatcac gttttccagc tcgcgcacgt
     1021 tgcccggcca gtcccacgac atcaactggc gtagcgcctc ggtcgggatg gcgtcaattg
     1081 aacgattcat atggcgcgcc attttttgcg tgaaatgctt tgctaaaaga gggatatctt
     1141 ccggacggtc gcgtagcggc ggcaattcca gtgggaagac attcaggcga taaaagagat
     1201 cgctgcgaaa ctggcgatct tcaaccattt gccacaaatc acggttggtg gcggcaatga
     1261 ctctgacatt caccgggatc gttctactcc cgccgagacg ctcaatctcc cgctcctgca
     1321 atacgcgcag cagtttaggc tgaagttcta acggcagatc gccaatttca tcaagaaaca
     1381 acgtgccgcc atcggcaatt tcaaaacgac cacgatgggt attaatcgca ccagtaaacg
     1441 cccctttgtc atgaccgaat aactcacttt ccagaagact ggcggggatg gcagcgcagt
     1501 tgattttgac cagcggcttg tcgcgtcgcg ggctaagttg atggatcgct ctggcgatca
     1561 cctctttgcc ggttccggtt tcaccacaaa tcaacactgt actgtcgctc ttcgccacaa
     1621 tatctacctg ctggagcagg tcttccattg cctggctttg atagataatg tcaccgacgc
     1681 ccagattcga aaggagctgc tcgctaagct ggtggttttc ttgctgcaaa ctttcctgca
     1741 aatcggtcat gctacgccag gcatcggcat tgccaacggc aatagcgatg cgatcggcta
     1801 tgtgttgtag tagctgacag ttttcctcac taaagagagt ggaagaggta tgcgccagca
     1861 acaatgcacc cggtgtatgg ttgccaaagg taagcggtat aaggagcgca gattcgcagc
     1921 cgttagatgc aagtaagagt aataacgggt cgcgttgcca gagaaacaga tcgtctgcac
     1981 ggtgcgtcaa ggtcggttgt cgggtttgta gcgtttgtgt cagcaataca ctgtcgccag
     2041 gcatattgcg tggcagacac gcacaatgtg aggcagaaag atcgctgcac cacaggctaa
     2101 acttctcgtt ctttcgatga tcgcccagta ccatactgac tgaagccaga ccgaaaaaat
     2161 gatggatctc acgagcgacg tcagcgatca gatcgtcgag atcaagatgt gacagcactg
     2221 cattagtaat atcaaccagt acccgatgat gatcgcgatc tttctccagc aaacgcagcg
     2281 ccacctgctg ttcttcatca ttgagccatg attgcagaac cggggtgatt atccgcgcca
     2341 gccagttaaa cagtgtgcaa tcctggggag taaaacggca gccgtgggtg cgaataaagc
     2401 gtagctccgc cattccacta ctgccgagaa ggacggcgtg acgcgaataa tcgaccatct
     2461 cgccctgata ctctgttgtt gccgtcacat cgctgccatc atcatggtag cggtaataga
     2521 agttgctttt gagaacatgc agttcaataa gttgtaccgg gctgaacggc ggcggcagcg
     2581 ggatgaaggc gcgtaataaa gacgccttac cgtgtttctg cgctaaccgc tccgcgagca
     2641 ttccgtttac cgcttcaatt gttattcctt gcggcggggc aaacatcgcc tcgtctgaca
     2701 tagccatcaa cagtccttat taatgagaaa tccttctcac gcaatttttc tggccatcag
     2761 atagatggcc ggttcctgca caatttcatc caacagatga atgaattgaa tgctacgcgc
     2821 ctgccacaca gacgtcgcgt catccgccag tggcgcgaat gcccgggtca acagggcggt
     2881 gtggctggcg tcaataacaa tctcaccaaa agtcatcacc cccagcatct tacgtcgggc
     2941 ttgctcctct tcgaccagtg caagccaatc ttcgtattcc gtcagtgggc aggggaaggc
     3001 gacattcaga caatcaatca cgccaacgtg atgaccgata gccagcgagt aatacattac
     3061 ctgagagctg tgttccggca tctcgtcgct actggcgaca aacttttttc gcagcgtcca
     3121 gaaaacaatt tccccgcact cttcagtcat gtaacccccg gagtacgcga aagagttgct
     3181 gaacgatact attgagacgt ggatcgtcgg catcgcgcaa ccaggtgtta acccgatttc
     3241 ctgtaggatc gtcgcttaaa tggcgcagga gccgatcgca aatttctcgc ccctgacgat
     3301 agcccgccag ccgccgcgct tctcgctcaa tggcgatacg ctgtgatggc gggatctgcg
     3361 gccacaacgg ttgcatagtc accccggtgg gatcgcgata atccaccgcg tgaatcttct
     3421 gttgcagcaa accgagcgcc acggcgaaac cgtgaatggt ggcggccggt gttggcgggc
     3481 agccggggat ccaaacatca atggggacaa tggtgtcgct gccgccccag acgctgtaga
     3541 gatcgtggaa aataccgccg ccgacaccgc acgcgccgta ggaaacacaa attttatgat
     3601 cgggggcaga ctcatacgcc cgaagtgcag gcatacgcat cgcccgggtg actgcgcctg
     3661 taaataacaa aatatcggcg tgacgcggtg atgaaataac cttaatgcca aaacgttctg
     3721 cgtcgaatac tggtgtgatg gcggcaaaaa tttcgatttc acaggcgttg cagccgccgc
     3781 aatcgacgcg ataaacgtaa gccgagcgac ggatatcctg tagcaaatgc tgcttcatct
     3841 tttgcgtttg ctcgtccagc gtgatgggct ggctgacatg ttgtgtaagc actggactca
     3901 tagctgctcc ttagccacca gcggtacatc tgtatcgtcg ttgagcagcg tcgcgcgttg
     3961 tttgcactcc gggcagacgc tcgcttgcgc ccataacatt tcgcggtttt gtggcgcatt
     4021 ttgttgctgt gccaacaatt cagcagccag tgcgacggtt ttttgcgggg caaacgggcg
     4081 ttcgcaacgg ctgcaacgtt gtagatggaa cgtcgcgcgg gtatagagat cggctttatt
     4141 ggtgacggtc agttcaaagt tattggtaag ctggatggct ctggtcgggc acacttcttc
     4201 acaacgtccg cagtaaatac aacgcctcag atagagctgc caggtgcgcg aattttgctg
     4261 gtcgtcggtc tggatagtca gcgcatttgc cggacaagca caggcgccgc aggcaataca
     4321 ttggctgggc atcaggtccg gtttcccgcg aaagcctggg ctgacctcca gtggcgcgaa
     4381 gggatatttc accgtcgcgg ttccggcgcg cataatagtt ttcagtaact tcagcatctt
     4441 ctgtccttac ttcagcggcg aacggttacg atcaatgccg tagcgttcga tctctttata
     4501 cggcacggtt tttgactggc gcttgcgcac atctaccagc gtcacacggt cggtacagga
     4561 gtagcaggga tcaaggctac cgataatcag cggtgcgtca gaaacggtat tgccgcgcag
     4621 catgtaacgc aacaccggcc agttggcgta ggtggcggca cggcagcgcc agcggaacaa
     4681 tttttggtta tcaccgagca tgctccagtg cacgtcttca ccgcgtggcg cttcaacaaa
     4741 gcccagcgcg aatgcgtgag gtttatagct aaagccttcg gtcagcagtg gggtatccgg
     4801 catgttgtcg agggcaaatt ccagcattgc cagcgaatca aacgtctctt tgacacggac
     4861 catcacgcgg gagaaaacat cgccgccggt aaaggtaaac agtgtttttg gaatattgcc
     4921 gtagtcggcg taggggtgat caaagcgcaa atcacgggca aaaccactgc cgcggatcag
     4981 cggccctaca gggctataat cacgggcgat ttgtcggtcg agaatgccaa tgccctgagt
     5041 gcgttgttcc atattcggcg tggcaagcag catctctacc agctccgaca cgtcggcgcg
     5101 catctcgcgc accagtttca gcgtttgcag acgttgctct ttgagaatat cgcggcgaac
     5161 accaccaatc agattcagac cgtaggtttt acgcgacccg gtcagcaatt ccgccattgt
     5221 catcgacttt tcccgcacgc ggaaaaattg cataaagccg gtatcaaatc caacaaaatg
     5281 gcaggagagg ccaaggttga gcaaatgact gtgcagacgt tcgacttcca gcagaatcga
     5341 gcgaatagta tgcgctcgtt gcggcacctc aatccccagt gcattttcaa ccgagttggt
     5401 ataggccaca ctgtgggcaa aaccgcaaat cccacacacg cgatccgata agaaggtcac
     5461 ttcgttgtag cccatccgcg tttctgccag tttctccatg ccgcgatgga cataaaacag
     5521 gcggtaatca gcatcgacaa tttgctcgcc atccacaaac aagcggaagt gacccggttc
     5581 atcggaggtg atatgcagcg ggccaatagg gatcacccgc gcatcgctat tgccctcatt
     5641 gataaacgga tacgtttcgg tatcagtcgt cggttcaggg cgcagtcgat aatccatcgc
     5701 atctttgcgc agcggatgca tatcttccgg ccagttatcg ggcaacacca ggcgacgctg
     5761 atccggcagg ccaaccggaa tcagcccgta catatcgcga atttctcgct cgccccagac
     5821 cgcggcaggg acgcgcggtg tgactgacgg aaactcccga ctgtcggcat cgaccagtgc
     5881 cttcaccacg atccagcatt tttcggcccc ttccattgaa agggcataat aaaccgcgta
     5941 atgaccgtta agtgtccgct cgtcgttgcc aaacaggact ggaagccagc catcatgttg
     6001 ataataaaga taatgtacaa cgtcaggcag cagattgatt ttcaccgtaa tggtgacctg
     6061 ttcaggcgtt tgtcgctctt catccagcac cgcgccgggg aactgcgttt tcagggcggc
     6121 gagaatcgct tcgccacgat ttgacgatga attaacgttc actgtgtttc tccgaaacag
     6181 atgcggtgcc tgaaggcaaa aaatcatgcc aggtgctacg ttgtgcaggc aggtcgtgcg
     6241 tccctgagag gactatagtg gaagcgcccg ccaggatcct gatgacaggt tgtggaatat
     6301 gcgttcccat cgccagcatc atgaccagca gaatcaccat tggcgaggtg gtcaaccagc
     6361 cgagatcacc ccggttaacg gcctgcggcg gtttcgccat taacacccgc gctgccatcc
     6421 gtaccaggcc cgccagcacc agcgttaaca gcaataacag caggacgata atcagcaggt
     6481 gattacgtgc cagtccggcg gtaatggtca taaattcgct aagaaaaatg ttgaagggcg
     6541 gcatccctgc cagcgccagc gcaccgccgc caaacagcac ggcggtaaat ggcatgattt
     6601 tgagcatccc acagacgacg ttgagatcgc gcgtgccgta cttgagcagt acattgccgg
     6661 aaccgcagaa cagcagcgtt tttgccagac tgtggtttaa gatgtgcagc agcgcggcaa
     6721 aaattcccag cggcccgcca atgcctagcg ccaccgcgac cagccccatg ttctccacgc
     6781 tggagtacgc cagcagacgc ttaatgtccc gctgtaccag aatgaaaaat gccgccacgg
     6841 caaccgacaa catgccgaag atgagcaaca accggttggg gaaatcgctg ccgatggctt
     6901 ggcaaataat gatgtaatag cgaatcagca ccaacagcgc gcagttcagc aatacggcgg
     6961 agagcagggc gctgaccgga ctcggcgctt cactgtgagc atccggcagc caggcgtgca
     7021 tgggaaatag cccggttttg gtaccaaagc caattagcac aaacacaaag gccaacagca
     7081 ttaatgttgg gtcaagcaag gacgattgct taagaacctc gctccagaat atcgccattt
     7141 ctgcctgcgg cataacgctg gcggcgttgg cgtataccag cacggtaccg aacagaccaa
     7201 aagcgacacc aacagtacaa ataatgatgt acttccatgc tgattccagc gatgaacgct
     7261 gaccgtaaat gcctaccaga aacgccgagc ttaaggtggt ggcttcgatc gccgcccaca
     7321 tcacaatcag gttattgctg gtaacaacca gcagcatggt gaacaaaaac agatggaaga
     7381 aaccgtagta atcgcacagc gtaacgggtg aaagctcgcc gtgtgccact tcatgacgca
     7441 tgtagccaat cgagtaaaca ccggtgagaa agccaatcac accaagaatg gcgaggaaca
     7501 aaccgcccag accatcaata tgaagccaca gtcccgcagc gaatatttct cctgcatcag
     7561 cggcagtttg gaccacccag agtgccagaa tcagcagcag tgtgatccct aagctatgta
     7621 atacagtcac cgtacaagtc gcagagagcc cccgtttccg gcaggcaaaa cagagcagcg
     7681 aaaaaagcag cggcgtgagc aggagtaaag cgaacatcac agaataactc atctcattat
     7741 cccttcagcg cggtcaagtt gttcacgtcc agcgtgccgt gggtacgcca tatttttctt
     7801 gccagtaaca ccatcacaat gacggcgaag atggcgtcgg tagcgatacc tatttccacc
     7861 agttccggtg ctcgccaggc aagaagcgcc agcaccagat gggagccgtt ttccatcagg
     7921 cagtaaccaa aaatttgccg caggatattg cgctggctga caatgcacag caggccaagc
     7981 agaaaatgac ctaacgctac cgccagcgcc ggtttcagcc cggtagccat cggtagcttc
     8041 acgggctgaa cgacaaatgc gcaaagcagg acaattaacg ccgcgagcag tgccatcatt
     8101 gccggaccga ataacgcttt ttccgggatg ttctggggaa tatttcgtgc agcgtaagtc
     8161 atgattaacg gtaccagcag cactttggtg ataaaggcgc tggcggacca gatcagcagt
     8221 tgctctgcgg cgaacaggca cgagagagtg gcgaaaatag acaccagcac cagcgactgg
     8281 caggcgtaaa atccgcatga caggcgatag cttttgacgc taatcacaaa cagcgatgtc
     8341 agcatcatca gtcccgccag attatttacg atcatagaac cggtcattca tcatctcctt
     8401 actgcaacca ggtcgcggcg attacgctgg aaatcagcga catcacaatc agtacaatca
     8461 acaccaggcg cattgaccct ggcagcggtg cggcatcggc gacggcctcg ctcggtttgc
     8521 ccggcacgac gcgaccaaac cagcgaataa accaggcgaa actggcgacc gattcaatca
     8581 tcagcagaat catggcgggc agcaggatcc agtactccac tgacaacgca aaaccgacgg
     8641 caaacagcgg gaatttactg aagaagccgt tgaacggcgg cacaccggta atcgccagcg
     8701 ctgccacgca gaaacccacg cttggcaacg gcagggtgtg caatacgcca cgcagacgcg
     8761 gcaacaagcg cgtgccgcag ctgtaactca gcgcacctgc tacaaggaaa aacaggcttt
     8821 tagcgaacgc gtggttgacg atgtaggcga tgctaccctc cagcgccagc cgcgagccga
     8881 agatggagag cgacaagccg aagaacatcc agccaagttg agtgatggtc gaccaggcca
     8941 gcaaccgctt catatcctgc tgtggcaaat acatcagaaa gccataaaga atggtgacca
     9001 gcgccatgac catgccaacg ccgccaatca catgcgggat attgccgccg tcgataatgg
     9061 cgcgggcaaa aatgtaaacg cccactttca ccatcgatgc ggcgtggaga taggcactga
     9121 tcggtgttgg cgcttccatt gcatccggta gccacgcttg catcggtagc tgggccgatt
     9181 tcccccacgc ggcaaacagg atgccgccat aaaccagata acgtgcgtcg ccgtgtaact
     9241 cgctcatcgc gctaagcgca aacgttccgg tctgcaaaaa cagcgtggcg gcggcaagat
     9301 acaaccccaa cgagccgata tgagtgataa gtaacgcttt tagtgctgaa agctgtgctt
     9361 tatcgctctg gtaataactg atcaacgccc aggagcagcc gcccgtaatt tcaaaaaaca
     9421 acaactgacc gagcagcgtc gaggagagta ccagtcccgc catcgcgccg ataaacacca
     9481 gcaaaaatgc gtaataacga ttcgtgccgt tatgcgggtg ttcgcgattt ttatccgtca
     9541 gataacccgt ggagtagatc gtgaccagca aaccgagaaa caccaccaca aacagaatca
     9601 gcgtactcat gcgatcaatg accaagccaa acaatgccac atcccccacg ttgaccaacg
     9661 tcaacgtgac ggcaacttta tcggcctgat aaaacgcgga gatcagcgac aacatgcaca
     9721 gcgtggtcag tgcggcgaac aaaaccccca ttcggcggcc cgacgttgtg gcgaaaacga
     9781 aacgaccagt gcgccgataa aaggcagcat taacgtcgtc agagcaagat tttccatatt
     9841 ccgtcagtgc tccttacaga ccggttaacc agaagaccca tgcaagcgca gcaaggctga
     9901 agccaagcca ggtcacatgg tgaatgagta aaaaacgccc gcgtgccagc gtgttttctg
     9961 cgattgaggc cagtacaaaa atcagcaaaa ccttgagcag cgtaacgaca agtgaagtca
    10021 gcaggcaggc gagagaaagt tcttgcgcgc gcccaaaggg cagaaacagg gccacaaaca
    10081 gtgctgccat cacgacctgt ttcagcccca gcccccattt cgctagcgcc agcccggcac
    10141 cggaatattc ggtcagcggg ccttcctgta attcctgttc tgcttcagca acatcaaagg
    10201 gaatttttcc catctcaatg aagcaggcaa aaccacaggc cagtaacgcc agtacggtgg
    10261 ttagcggcga gttccagccc gttgccagcg tattgctgat catctcgata tgcgtggaac
    10321 ctgctatcag cgccaatacc agcagtgaga gaatgagcat gggttcgacc agaatgccga
    10381 gcgtcaactc gcgactggca ccgactcccg caaacgggct tccggtatcc agccctgaaa
    10441 gagcaaagaa aaaacgaaac agggcaagaa gatagataag ggtgatcaga tcgccgccgc
    10501 ccgcaaaagg ggaaacggta ataaatagtg gtagggccat cgccagcacc agcatgctgc
    10561 tgattaatac ccacggcatc aggcggaaca tcagacctga agatgtcggc gcatcttcct
    10621 ggcgtttaaa cagtttgtgg atatcgcgat aatcctgcca gatccccggc ccgcggcggg
    10681 agtgcatacg cgcgcgtatc tgccgggaaa tacccgtaaa aagtggggtt agcatcagca
    10741 gaatcacggc ctgtgctaac gcaaaaatga ccagatatcc gtcgcaaaga gtttgtctca
    10801 tggtgatttc cttagacggc aatagcgatt agcagcacaa ccagtgcggc gaccacatac
    10861 aggcaataga gacgaaagtc gccgctttgc agatgctgta tttctttggc cagccgttgg
    10921 gtggcgctca cgatggggcg gatcacccgc tcatcccaga agggttctgt gctctgagcc
    10981 ctggcggtga cgtgcgcaag acctttattt agcctcagcg tagggtcgag ctgttgtcgt
    11041 agacgaaata gcgcagaaaa gaccacacgc agcggctgca tcacgccatt gcctgacggg
    11101 gccatcgcat tttcccagcc gtagccgcat gcccaggcat ctcctgtgcg acgaaacgct
    11161 gcgcggcgcg aacgacacca cagccagtaa aggccaggca gcaaaggcat cgccagtagt
    11221 aacagcaaca gtaatgaagg agtgacccgt gtatgaaacg tgccgggtac aagtgctatt
    11281 ccgctggcga cagtgacggg aggggtattg gtaaacgcat gggcaatgtg cattatcttc
    11341 ggtgccagcc aactggcact aagcgcaatt aatacgcaga gtgccgcgag cagtagcatg
    11401 gcgacgatca tcgtatttgg cacttcctgt gcctcttcag cgtgtgtgct gcgcggcgca
    11461 ccacagaaag taataccgta cattttgacg aagcacatta ctgccagccc accagtgact
    11521 gccagcatta ccatagcaat aggacccgca agttgtagcg ctacggcttc cacacgactt
    11581 agtgagaata gcgactgcca ggtgtaccat tcgctgataa aaccattcag cggaggaatg
    11641 gctgatatcg ccaggcaacc aatcaggcat gctgcggctg tccacggcat ccgtttcgcc
    11701 agtgccccca ttttttccat gtcgtgggta tgcaaacgcg aaataatcgc acccgcgccg
    11761 agaaatagca gccctttgaa aagcgcatgg ttgagcagat gaaacagtgc gccgagcagt
    11821 ccaaccacgg tgagcagcgg gtcgtgcagt gacagaccga ccatcgccac accgaccgcc
    11881 agcaaaataa tgccgacgtt ttcgacggta ctccaggcca gcagccgttt gatatcctgt
    11941 tccgccagtg catacagcac gcccaggagc gcggagattg cgccgatcgc catcaccaga
    12001 atgccccacc acagcggcaa acccgtttgc gccagcagat ccatcgccac tttcaggatg
    12061 ccgaaaatac cgattttgac cattacgcca gacatcaacg ctgaagcgtg cgatggtgcg
    12121 gcagggtgag cgcgcggcaa ccagctgtgc aacggcatca tcccggcttt cgcgccaaaa
    12181 ccgaaaaagg ccagcaggaa caccgccgac gccagccccg gagaaagtga aagcgtgcgg
    12241 aaactggcaa aatcgaggct gccgctttcg cgccccatca gcaagaaggc gatcattatc
    12301 agcaccgatc cggcgtgggc gataaaaaag tagagcatgc cagcgttgat cgacgtttta
    12361 tcctgcctgg caatgaccag aaaccaggaa gacagcgaca tcatttcaaa cagcacgatg
    12421 aaccaaaaag cgttgtccat caccagcagg gcaaccatcg atgcgatgaa aatattcata
    12481 aagaagccca tcgccgccgc gcctttgccc tcgtattcgc gcatataagt caatgaatag
    12541 agagaacaaa ccaccaccag caaggagata accagcacca taaatgccga gaggctatcc
    12601 attcgcaggg ttaaatcggc aaattcaaaa ggcgtggcaa aacgggcgac taaagttacg
    12661 cctgcatgta attgcgtaat tccgctgatg atgccaatca cgccgcccac cagcgatgtg
    12721 atgccggaaa gcttaatagc cagtctgtcc agaccgagta aaaacagcga agccagactg
    12781 gcaaacagat agaggatcag cgaccaggtt aataattgca gggcatccat tagcgttgct
    12841 cctgagtgag ggaaagggga tcctccccgc cattggcaaa ccagcttgcg gcaaggcgct
    12901 gtttttcttt catctggcgt tgcaggctat cgtcggtgat cagccgtaac gcctgattcg
    12961 ggcaagcgcg aacgcaggcc ggaccttctg gcaagaaatc acacaggtcg catttcaccg
    13021 cgacggtctg gacacccggt tcccagcgca gcaacgcatg ttggggcggc acgttttctt
    13081 cgccgtcttt gagtgagccc tcagcctgaa aaacatattg cgcaggggca ttcaccggac
    13141 ggcttcctga agcgctgatt gcgccaaatg ggcagaccac ggcgcaaagc ttgcagccaa
    13201 tacagaggct ttcgttgagt tggatcgcat catccctctg agagatggca ttgaccgggc
    13261 agacctgcag gcaaggggct tcctcacagt gatgacacac gacaggggca gtgagtgttg
    13321 acgtcttcgc cagggccagg cgcgggtgtt gctgtaaacc ttgcgtttta tgcacgtccg
    13381 aacaggcagc gagacaggta ttacatcctg tacaccacag tggttcggcc accacaaagc
    13441 ggttcatgtc ggctccttga aaatgatatg aaacattgca tttcatattg cataaaggat
    13501 gctattctga ttaactcaat atattcaata agttatgtat taactggatt ctgaacggta
    13561 caaaaatgat ggtcattaac gttcagcaaa aaatggattc gacacccgtg tcgcagggga
    13621 aagcgagaat gtgatcttga tcacgagaag tgaggtatgc tggccgcaag cgcagccagc
    13681 acggaatgga gcaaagtaat caggcgtgtt ctttcagcca gttcagcaca acgtcgtggt
    13741 gattgctggt tttgaaatcg tcaaagacat gttcgatttt gccatcagcg tcaatcagga
    13801 agctgatgcg atgaatgcca tcgtaggttt tgcccatgaa ggacttttca ccccagacgc
    13861 cgaattgttc gcacacctgg tggtcctcat cagacaggag cgtaaagtta agcagctctt
    13921 tttccgcaaa acgggagagt ttttcgggtt tatcggtgct gatacccagc acatcaacgc
    13981 ccgctttttt caactcatcc atgttatcgc gtaagccgca ggcctgtacg gtacagccgg
    14041 gggtcatggc tttcgggtag aaataaacca gaacacgctg tccctggaag tcggtcaaat
    14101 taacttgttc tccgtcttga tccggcaagc taaatttcgg tgcgatatca ccggctttca
    14161 gtggattcat tacttaactc catcctgttc atcatgttgg gaataattga cgacgttaat
    14221 actgccttgt gcattgagtt ctgtacatag ggctttgaac gcttgctcaa tatttgctgc
    14281 gtccgcagat gcggggctgt gggcggttat ctgaatatgc aactgcgcag cccgttcatt
    14341 ttcagcaggt tgcgtgcgcg acaccagctc cgcaatgttc atatgatgcg cgtcgaaaag
    14401 tgctgtgaag cgttcaatta aatgcgggga gtctgccaca tcgacctgaa cccagacaga
    14461 tgctggcatt ggcggacgcg gacgcgccgt cgtgcgcttc atcacgatta aaagatccag
    14521 ctcggcacct ttcaacggta acgttgattc aatcagagta atggcattcc atgaaccgga
    14581 aagcagcatg ataaacgtga actcttctcc cagcatcgcc aggcgactgt cttcaatatt
    14641 acagccgcaa ctactgacat gacgggtgat ggtgttcaca attccagggc gatcggcacc
    14701 caatgcagtg atcaccagat aatgttgcga tgacagtgtc aaactggtta ttcctttaag
    14761 gggtgagttg ttcttaagga aagcataaaa aaaacatgca tacaacaatc agaacggttc
    14821 tgtctgcttg cttttaatgc cataccaaac gtaccattga gacacttgtt tgcacagagg
    14881 atggcccatg ttcacgggaa gtattgtcgc gattgttact ccgatggatg aaaaaggtaa
    14941 tgtctgtcgg gctagcttga aaaaactgat tgattatcat gtcgccagcg gtacttcggc
    15001 gatcgtttct gttggcacca ctggcgagtc cgctacctta aatcatgacg aacatgctga
    15061 tgtggtgatg atgacgctgg agctggctga tgggcgcatt ccggtaattg ccgggaccgg
    15121 cgctaacgct actgcggaag ccattagcct gacgcagcgc ttcaatgaca gtggtatcgt
    15181 cggctgcctg acggtaaccc cttactacaa tcgtccgtcg caagaaggtt tgtatcagca
    15241 tttcaaagcc atcgctgagc atactgacct gccgcaaatt ctgtataatg tgccgtcccg
    15301 tactggctgc gatctgctcc cggaaacggt gggccgtctg gcgaaagtaa aaaatattat
    15361 cggaatcaaa gaggcaacag ggaacttaac gcgtgtaaac cagatcaaag agctggtttc
    15421 agatgatttt gttctgctga gcggcgatga tgcgagcgcg ctggacttca tgcaattagg
    15481 cggtcatggg gttatttccg ttacggctaa cgtcgcagcg cgtgatatgg cccagatgtg
    15541 caaactggca gcagaagggc attttgccga ggcacgcgtt attaatcagc gtctgatgcc
    15601 attacacaac aaactatttg tcgaacccaa tccaatcccg gtgaaatggg catgtaagga
    15661 actgggtctt gtggcgaccg atacgctgcg cctgccaatg acaccaatca ccgacagtgg
    15721 tcgtgagacg gtcagagcgg cgcttaagca tgccggtttg ctgtaaagtt tagggagatt
    15781 tgatggctta ctctgttcaa aagtcgcgcc tggcaaaggt tgcgggtgtt tcgcttgttt
    15841 tattactcgc tgcctgtagt tctgactcac gctataagcg tcaggtcagt ggtgatgaag
    15901 cctacctgga agcggcaccg cttgcggagc ttcatgcccc ggctggaatg attttgccgg
    15961 tgacctccgg tgattatgca atcccggtga ccaacggtag tggtgctgtc ggtaaggcgc
    16021 tggacattcg tccaccagcc cagccgctgg cactggtttc tggcgcgcgt acccagttca
    16081 cgggcgatac cgcttcattg ctggtggaaa atggtcgtgg caatactctg tggccgcagg
    16141 tggttagcgt gctgcaggcg aaaaactaca ccatcaccca acgtgatgat gctggtcaga
    16201 cactgaccac cgattgggta caatggaacc gtctggacga agacgagcag tatcgtggtc
    16261 gttatcaaat ctctgttaag ccgcagggtt atcagcaggc ggttacggtt aaactgttga
    16321 acctggaaca ggcgggcaaa ccggttgcag acgcggcttc catgcagcgt tacagcacgg
    16381 agatgatgaa cgttatttcc gccggtctgg ataaatctgc cactgacgcc gcgaacgctg
    16441 cgcaaaatcg tgcctccacc actatggacg tacaaagtgc agctgatgac accggtttac
    16501 caatgctggt cgtacgcggg ccgttcaatg tggtttggca acgtctgcca gcggcgctgg
    16561 aaaaagtggg catgaaagtg accgacagca cccgttcgca gggcaacatg gccgtaactt
    16621 ataagccgct gtctgacagc gactggcagg aactgggcgc gagcgatcca ggcctggcat
    16681 ccggtgacta taaactgcag gttggcgatt tagataaccg cagcagccta cagttcatcg
    16741 atccgaaagg tcatactctg actcagagtc agaacgacgc gctggtagct gtcttccagg
    16801 ctgcgtttag caagtaaaaa tacagggctg gaatcatccg gccctttttt ctgatatgat
    16861 acgcaaacgt gtgcgtcggc aggaaaacgc gattttagcg gtaattcgca cgaaatttgt
    16921 ttgtcggacg tagttcggat aaggcgttca cgccgcatcc gacaaaacat ccggcacacc
    16981 agacagcaaa agattttaaa acgttaattc acacccagga gtgataaaga tgcaaaagca
    17041 agctgagttg tatcgtggta aagcgaaaac cgtatacagc acggaaaacc cggacctgtt
    17101 ggtgctcgaa ttccgcaatg atacgtcagc aggggatggc gcgcgcattg agcagtttga
    17161 tcgcaaaggt atgttgaaca acaagttcaa ctacttcatt atgagcaaac tggctgaagc
    17221 gggtatcccg actcaaatgg agcgtctgct ctccgatacc gaatgtctgg tgaaaaagct
    17281 ggatatggtg ccggttgagt gtgtcgtgcg taaccgtgct gcgggctctc tggtgaaacg
    17341 tcttggaatc gaagaaggta ttgagctgaa cccgccgctg ttcgatctgt tcctgaaaaa
    17401 cgatgccatg cacgatccga tggtcaacga atcttactgc gaaacctttg gctgggtgag
    17461 caaagagaac ctggcgcgta tgaaagagct gacctacaaa gcgaacgacg tgctgaaaaa
    17521 actgttcgat gatgctggtc tgattctggt cgacttcaag ctggagtttg gtctgtacaa
    17581 aggtgaagtg gtactgggcg atgagttctc cccggacggc agccgcctgt gggacaaaga
    17641 aacactggag aaaatggaca aagaccgttt ccgccagagc ctcggtggcc tgatcgaagc
    17701 ctatgaagcc gtcgcccgcc gcctgggtgt acagctggac tgatttttct gttcatcatc
    17761 ttgccgtgca gctggcacgg caagacaacc gctctcggaa tatatgcatt gaacgctcat
    17821 tcctccgcca tttcatcccg ctttcctgtg gtaatcttcc tctgaaccac ctacgatcat
    17881 ttctataatg aatatattgt tgaggtatct atgcgttggc aagggcgacg tgaaagtgac
    17941 aatgtcgaag acaggcgtaa cagctccggc ggtccgtcaa tgggcggtcc cggttttcgc
    18001 ctgccaagcg gtaaaggcgg gctgatttta ctgatagtcg tgctggttgc aggctactat
    18061 ggtgttgatt taaccgggtt gatgaccgga cagccggttt cccaacagca atcaacacgg
    18121 tcaattagcc caaatgaaga cgaagccgca aaattcacct cggtgattct ggcaaccacg
    18181 gaagacacct ggggacagca attcgagaag atgggtaaaa cctatcagca accgaagctg
    18241 gtcatgtatc gtggaatgac gcgtaccggc tgcggggcgg gccagtccat tatgggaccg
    18301 ttctattgcc cggcggatgg cacggtttat atcgatctct ccttctatga tgacatgaaa
    18361 gacaaactcg gcgcggatgg cgattttgcc caggggtacg ttatcgccca tgaagtcggt
    18421 catcatgtgc agaaattgtt aggcatcgag ccgaaagttc gtcaactgca acaaaacgcg
    18481 acgcaggcgg aagtaaaccg cttatctgtg cgtatggaac tccaggccga ctgttttgcc
    18541 ggtgtctggg ggcataacat gcagcagcaa ggcgatctgg aagaggcgct gaacgcggcg
    18601 caggccatcg gcgatgaccg tttacaacag caaagtcagg ggcgagtagt accagacagt
    18661 ttcactcatg gtacttctca gcaacgctac agctggttta aacgtggttt cgacagcggc
    18721 gatccggcac aatgcaatac ttttggtaaa agcatttaac ttccggggca aggatggctg
    18781 aactgactgc gcttcacaca ttaacagcgc aaatgaaacg tgaagggatc cgccgcttgc
    18841 tggtgttgag cggggaagag gggtggtgtt ttgatcatgc gcttaagttg cgtgatgcct
    18901 tacctggcga ctggctgtgg atttcgccgc agccagatgc tgaaaaccac tgttctccct
    18961 cggcgctaca aactttactt gggcgcgagt tccggcatgc ggtattcgac gcccgccacg
    19021 gctttgatgc cgctgccttt gcggcactta gcggaacgtt gaaagcggga agctggctgg
    19081 ttttgttact ccctgtatgg gaagagtggg aaaaccaacc tgatgccgac tcgctgcgct
    19141 ggagtgattg ccctgaccct attgcgacgc cgcattttgt ccagcatctc aaacgcgtac
    19201 ttacggcgga taacgacgct attctctggc ggcaaaacca gccgttctcg ttggcgcatt
    19261 ttaccccccg tactgactgg caccccgcga ccggcgcacc acaaccagaa caacagcaac
    19321 tcttacagca gctactgacc atgccgccgg gcgtggcagc ggtaacggct gcgcgtgggc
    19381 gcggtaagtc cgcgctggca gggcaactca tttctcgtat tgcgggcagt gcgattgtca
    19441 ccgcgcccgc aaaagcggca acggatgtac tggcacaatt tgcgggcgag aagtttcgct
    19501 ttattgcgcc ggatgccttg ttagccagcg atgagcaagc cgactggctg gtggtcgatg
    19561 aagccgcagc catacctgcg ccgttgttgc atcaactggt atcgcgtttt cctcgaacgt
    19621 tgttaaccac tacggtgcag ggctacgaag gtaccggacg tggttttttg ctgaaatttt
    19681 gcgctcgctt tccgcattta caccgttttg aactgcaaca gccgatccgc tgggcacagg
    19741 gatgcccgct ggaaaaaatg gttagtgagg cactggtttt taacgatgaa aacttcaccc
    19801 atacaccaca aggcaatatc gtcatttccg catttgaaca aacgttatgg cgaagcgatc
    19861 cagaaacgcc gttaaaggtt tatcagttat tgtctggtgc gcactaccgg acctcgccgc
    19921 tggatttacg ccggatgatg gatgcaccag ggcaacattt tttacaggcg gctggcgaaa
    19981 acgagattgc cggggcgttg tggctggtgg atgagggcgg attatctcaa gaactcagtc
    20041 aggcggtatg ggcaggtttt cgtcgcccgc ggggtaatct ggtggcccag tcgctggcgg
    20101 cgcacggcag caatccactg gcggcgacat tgcgcggacg gcgggtcagc cggattgcag
    20161 tccatccggc gcgtcagcgc gaaggcgttg ggcaacagct cattgccagc gctttgcaat
    20221 ataggcctgg cctcgactat ctttcggtga gttttggtta caccggggag ttatggcgtt
    20281 tctggcaacg ctgcggtttt gtgctggtgc gaatgggtaa tcatcgtgaa gccagcagcg
    20341 gttgctatac ggcgatggca ctgttaccga tgagtgatgc gggtaaacag ctggctgaac
    20401 gtgagcatta ccgtttacgt cgcgatgcgc aagctctcgc gcagtggaat ggcgaaacgc
    20461 ttcctgttga tccactaaac aatgccgtcc tttctgacga cgactggctt gaactggccg
    20521 gttttgcttt cgctcatcgt ccgctattaa cgtcgttagg ttgcttattg cgtctgctac
    20581 aaaccagtga actggcatta ccggcgctgc gtgggcgttt acagaaaaac accagtgacg
    20641 cgcagttatg taccacactt aaactttcag gccgcaagat gttactggtt cgccagcggg
    20701 aagaggccgc gcaggcgctg ttcgcactta atgaggttcg cactgagcgt ctgcgcgatc
    20761 gcataacgca atggcaattt tttcactgac tccttcaatt ttctcccatg gtcattgtga
    20821 gtcagcggcg taaaattaat gacatcagtt aaggagttcg ccatgaaaca tgaccatttt
    20881 gttgttcaaa gcccggataa acctgcgcaa cagttgttac tgcttttcca cggtgtcggg
    20941 gataacccgg tggcaatggg ggaaataggc agctggtttg cgccgctatt tcctgatgcg
    21001 ctggtggtga gtgtcggagg cgcggagccg agcggtaatc cggcggggcg tcagtggttt
    21061 tcggtgcagg gtattacgga agataatcgc caggcgcgtg tggatgccat catgccgacg
    21121 tttattgaga tggtgcgcta ctggcagaaa cagagcggag taggggctaa cgccacagcg
    21181 ctaatcggtt tctctcaagg cgcgattatg gcgctggaga gcattaaagc cgaaccgggc
    21241 cttgcttcac gcgtcattgc ttttaacgga cgctatgcga gcctgccgga aactgcgtct
    21301 acagccacga caattcacct gattcatggc ggtgaagatc cggttatcga tctggcgcat
    21361 gcggttgcgg cgcaggaagc gttaatcagt gccggtggtg acgtgacgct ggatatcgtg
    21421 gaggatttag gccatgcgat cgacaatcgc agtatgcaat ttgccctgga tcacttgcgt
    21481 tataccattc cgaaacatta tttcgacgaa gcgctaagtg gcggtaagcc tggcgatgat
    21541 gatgtgattg aaatgatgta aaagacagat tacctggcct gataagacgc gtgagcgtcg
    21601 catcaggcat cgttcaccat tgccggatgc ggcgttaacg ccttatccgg cctacggttg
    21661 agcactcagc ttacttcttc ggttgatcct ttttcggcca gtcgtcgtca tcgtcccact
    21721 tatcgttgaa atcacgatgc gggggaagct ccggcttgtt ggcgagaaat ttcttgtggt
    21781 cgacgcgctt gagatcttta atcacgttca gcaggacgcc taccaaaaag acaatcacca
    21841 gaatccacca atatttagcc agccagtcca tgcttatttc ctcttgcaga accactcatc
    21901 aggcgacgag ctgttccatg atacgttgat acatacgggc aagtagctgc aggtcggcag
    21961 cgttcacaca ttcattaatt ttatgaatag tggcattgac cggcccgagt tccaccacct
    22021 gcgcccccat gcgagcaata aagcgcccgt cggacgttcc gcctgtggtc agtagctgcg
    22081 gtttaatttc attatagtgc tcaaccgcgt taacgaccgc atccaccagt ttaccgcgcg
    22141 cggtcaaaaa tggctgcccg gaaagccacc aatccaccgt atagcgcagt tgatgttttt
    22201 caagcagggc aagcacctgc gctttgatca tctcatcagt cagttcggtg ctgaagcgga
    22261 agttaaactg cacaaacagt tcacccggaa taacgttgtt actgcccgtt cccgcctgaa
    22321 tattggcaat ctgcatactg gtcgccggga agaattcatt gccctgatcc cactcaatag
    22381 ccactaattc attaaggaaa ggtgctgcgc gatgtactgg attgtcagcc agatgtgggt
    22441 aggccacatg cccctgcacg ccatgaatgg taaggttgca ggttaacgat ccgcgacgac
    22501 catttttcac cacatcacca accacttcga tactcgacgg ttcgccgacc agacagtaat
    22561 cgagacgctc attacgcgcc attaacgctt cgacgacttt taccgtaccg ttgtgggcac
    22621 tggcttcttc atcagaggtg atcagaaatg ccagtcgtcc cgtatggttg ggatgttgtg
    22681 cgacaaaacg ttctgctgct accaccatcg ccgccagcga gcctttcata tctgccgcac
    22741 cgcgcccgaa taacatgcca tcacgaatgg tgggttcaaa cggcggattg atccaacgat
    22801 cggcgtcgcc aggcggcacc acgtcggtat gcccggcaaa ggccaatgtt tcaccttgcc
    22861 cacgccatgc ccaaaaattc tgcgtatcgg caaagtccat gcgttcaacg gtaaaaccga
    22921 tcgcctgcaa acgatcaatc aacaaagcct ggcatcctgc gtcatcagga ctcagggaag
    22981 ggcggcgaat aagctgttgt gtcagctcaa taaccgggca cgacatagac tacacctcat
    23041 ggaaaaattg ctgataactg gaatcactga aacccagcag cataggctta ccgggcgcgc
    23101 agagcaatgg acgtttgata attgcaggca tttcagtcat taatgccgcc gctgaggccg
    23161 catcggtgat tttattgcgg gtggtttcgt ccagtttacg ccaggttgta ccacgggtgt
    23221 tgagtaacgc ctcccaacct aattcgttga taaaaccgtt caataattcg ctgtccagcc
    23281 catcgacgcg gtaatcatga aaacgatagt cgatgttatt ggcttccagc caacggcgag
    23341 cctttttaat ggtgtcacaa tttttgatac cgtaaagtgt aaccataatg aatccttttt
    23401 cgatttatga gaataaatac tcatttaagg gtgaaaacat tttgcctgca aatgattatc
    23461 aggaaatata acattatcga agtattcaaa ttaacaacta ttaaataata ttaaacattt
    23521 ttaacgtaaa cattaagaat attcatcttg ttatctgtct gtattttaac ctgaattaaa
    23581 tcagtattag atgccgtatg aatatctcga aatgttgctg tgtatcacat acaaatcatc
    23641 accgcaggac catagtattt acataagatt tgtccccccg gaggtagtca tcgcagcaat
    23701 cgagtaaatt agcttcacca cggtatattg tttccgtaga atacccataa ttataagaga
    23761 ggttgttatg attgaacgtg aactggggaa ctggaaagac tttatcgaag ttatgcttcg
    23821 taagtaattc ctgagacaaa gtataaacag caagaacccg ctgagcaggt tcttaatcgg
    23881 aaagttgaaa aaggcgacac attggcatgt cgcctttttt attgcttatt ccgggcgcgg
    23941 ctttagcggg aagcggcggc gcaccagcac aaagaacagc ggcacgaagt aaatagccag
    24001 aatagtggct gaaatcatcc cgcccattac gccagtaccc accgcatgct gaccaccgga
    24061 accggcaccc gtgctggttg ccattggcaa tacgccgaag ataaatgcca gcgaggtcat
    24121 cagaatcggg cgtaaacgct gacggcaggc gtggagcgtc gcttcaaaca ggtcgtggcc
    24181 tttttggttc atctcgttag caaactcgac gatcaggatg gcgtttttcg ccgataaacc
    24241 aatgaccgtt aacaggccca cctggaagta aacgtcgttt tccagcccgc gcatccaggt
    24301 tgccagcagc gcgccgatta cccccagcgg cacgaccagc attaccgaga acggcaccga
    24361 ccagctttca tacaacgcag ccagacacag gaataccacc agcaaggaaa tggcgtacag
    24421 cgccggagcc tgcgcgccgg aaagtcgctc ctgatacgac atcgccgtcc actccagacc
    24481 aaagccgttt ggcagctgct tcactaacga ttccataata tccatcgccg taccggtact
    24541 gacccccggt gcggcttccc caacaatctc aaccgcagaa taaccgttat agcgttccag
    24601 acgcggcgag cctgtttccc agcgtgaggt cgcgaaagca gagaagggca ccatgccgcc
    24661 atctttattt cggacatacc agagattgat gtcatctggc agcatgcgat acggcgcagc
    24721 tgcctgcaca tagactttct tcacgcgacc gcgatccata aagtcattca catagctcga
    24781 accccaggcg gtttgcagtg tgtcgttaat atcgtcgata gcaacgccca gcgcctgagc
    24841 tttacgctgg tcgatatcaa tctgcaactg cggactgtcg tcgaggccgt tatggcgcac
    24901 acgggttagc tccgggtttt ccgccgccag cgccagcaac tggttacgtg ctgccatcag
    24961 cgcatcgtga cctgctccag cgtggtcctg caactccata tcaaaacccg cagaactacc
    25021 aagaccgcta attgctggcg ggctgctggc gataacgcga gcttctttaa tcttgttaaa
    25081 cgcctttgtt gcacgctcga taatggcaaa cgaggtgccg gtcttactgt cgcgttcgct
    25141 ccagtctttc aggcggataa acattcgcgc cacgttttgc ccgttacccc cagggccaga
    25201 accaacggtg gcaaacaccg acatgatgtt gtctttttca tgggtgaagt aatatttctc
    25261 gatttgctca acgactttca gggtctgttg ttgggttgaa ccgctgggca attgtaccga
    25321 ggtagtaaac atgccacggt cttccagcgg taagaacgac gtcggcaaac gcaggaacag
    25381 gaacaccatg ccgccaagca gcaggacata aatcacaatc cagcgcaggc tgcggtggag
    25441 aattttcgcc accccttttt cgtagcgttc ggcgttgcgg ttaaacatct ggttaaacca
    25501 ggcaaaaaag cctttttgcc cgtgatgctc accttttttc agtggcttaa gtagtgtggc
    25561 acacagagcc ggagtgagga tcatcgctac cagtactgac agcaccatcg ccgcaacaat
    25621 ggtaatagag aactggcgat agatggcacc ggtggtgccg ccgaagaagg ccattggtac
    25681 aaataccgcc gaaagcacca tcgcaatccc gaccagtgcc ccctggatct gccccatcga
    25741 tttacgtgtg gcttcgcgag gagtgagtcc ttcctcactc ataatacgtt cgatgttttc
    25801 caccaccacg attgcgtcat ccaccagcag accgatcgcc agtaccatcg cgaacatggt
    25861 taaggtgttg acgctgtaac cgaaggcgta aagtacggag aaggttccca tcaacaccac
    25921 cggcacggcg atagtgggta tcagcgtggc gcggaagttt tgcaggaaca gatacataac
    25981 gaggaaaacc agggcgatag cttccagcag cgttttcacc acgtcttcaa tggaggcttt
    26041 aacaaacgag gtggtttcat acgccacctt gtattccagt ccgtgcggga agtactgcgc
    26101 cagctcgtcg agacgattga gcaccagctc cgctgtcgcc atttcgtttg cgccggaggc
    26161 cagttttacc cccagcccgg aggctggctt accattgaag cggctaagat aatcgtattt
    26221 ctccgccccc atttcgacgg tggcgacatc gcccagcctt acctctgagc cgtcttgatt
    26281 cacccgcaag gtgatatcgc ggaactgttc tggtgtttgc agcagtgact gggcgttaat
    26341 ggtggcgttg agcgcctgct tatcgacgga aggtgtacca ccaagttgcc caaccgcaat
    26401 ctgcgcgttc tgtgactcaa tggcatcagt gacatcttta gccgtcatct ggaaactgtt
    26461 gagtttcgcc gggtccagcc agatacgcat ggaatattgc gaaccatagg catcgatatc
    26521 cccgacgcca ttcacgcggc ttaacgggtc ctgaatatta ctggcaacat aatcggcaat
    26581 atcctgttta tccatcgaac catcggtaga gacgaaagca atggtcagaa tattggtatc
    26641 gccggtttta cgcaccgtca cgccctgatt ttgcaccgcc tgcggtaact ttcgcatggc
    26701 tgattgcagc tggttttgta cttgctgcac ggcttcgtcc ggatcggtgc ctgctttaaa
    26761 acttaaagtg acagatgcct gaccgatgcc actgctctga gatgacatat acatgagatt
    26821 atcgaggccg gtcatatttt gctcgataac ctgggtcacg gtgttttcca gcgtctgggc
    26881 cgatgcgccg ggatagttag cggtcactcg cacattcggt ggcgcgagat cggggtattg
    26941 ttcaacgggc aatgaaaaaa tcgccagggt ccctgtcaga cacaacagga ttgccagcac
    27001 ccaggcaaaa atggggcgat caataaagaa attcgccatt aaaagaggac ctcgtgtttc
    27061 tgcgtatcgt tgtaggcttg acttgcttca ctgtagcggc aaggtacgag ccaatcgtgg
    27121 agaaaaaaag gagttaatgt aaattatcat gcccttttat actgaacctt aagtgcaagt
    27181 attctttggt cagtaggagg cattacatca actgactttc ctcaccctcc gcagagcgaa
    27241 agctaatact cactaacgtg ccgccgccgg aaggttgtga aaaagtcagc gtcccaccca
    27301 gccgttccgc gcgttcgcgc atgatattca gaccataatg accttcgggt tctttcggtt
    27361 caccgatacc aatcccgtta tcacggatat aaaccgtgtg attgccgtcc ggcgcggtga
    27421 cgcaactgac ggcgatttcg ctggcgttgg cgtgcttcat cgcattcagc accgcttcgc
    27481 gaataatttg caacaaatgc acctgcattt gcgcatccag cgccagggtt ggcagacggc
    27541 agtcgagggt cagtttggcg ctggtttgat tttgtaacgt atccagcatt tccctcaacg
    27601 cggaggggag atccgcctgc tgcagcgtca ggcggaaagt agtcaacagc tcgcgtaact
    27661 gccgataagc atcattcaac gcctgggaaa agtcggccat gatactttgt gcggtggcgt
    27721 tatcttccgg tatcgaacgc ttcagtaacg tcaactggat acgtaagtaa gaaagtacct
    27781 gagccagcga gtcgtgcaat tcgcgggcga tggttgcacg ttcttccatc aacaataact
    27841 gctgaaaatg cttttgcgcc tgattaaagt acaaaccgcg tcccagcatc gacgaaacgc
    27901 tgttaagcag cggttctgat gatgaaacgt gactattttg ccagtgcagt tcgccgtaaa
    27961 ccgtctcttg cattgtcacc ggtaaaatct gcatcggcaa ttccgggttt ggttgccctt
    28021 cgctaatccg ccagttttca ccgacattta actccagata ttcagccgct tcattgtcgc
    28081 gaacaatctg caaaatatgg cggaaacaat gcacatcaat ctggcttgtg ttcagcgcct
    28141 gcgaacattg atacaacacc tccagacgac gcttggcctc gtggagatcg cgggtctttt
    28201 cttctactga cgcctccagc gaaaggtaca atttatgcag ctcgctcgac atctggttaa
    28261 aggtttttgc aagcagacca agctcattcg gcagactggt atccagcggc ggcgagtcga
    28321 actgcccgtg ttcaatacgt tgactggcgg taaccagctg attcagcggg gcaaccacct
    28381 gatggcgtat acggcgcaaa gtaaaaaaga ccagcgtgaa aataccgata ccgccagcca
    28441 gggaaatcgc caccaccagc agcattttgc gttcagcgta gtgctgtaaa gccagcacga
    28501 acaggtctat ctgattaaca taattattaa tattggcctg ataccacggc aaatcgccct
    28561 tgctgagccg attattcatc tccagccagt tggcattcag atgcgcatag cgagttttta
    28621 ctgcttctgg cacataccag acgttgagat tggttaatac cgatgaatgc agtgcctgct
    28681 gaaacaactg gcgatgtgca ttgagttgtg gactgccact ttgcaagtcg tagcccaggc
    28741 ggtaactctg catacgcagc gatccggcaa tattgatagc ctcagcgtcg cgcaaactgc
    28801 tcgccagagt tagcagagcg atacccgtgg aaagaatcga cagcagcaca atgtaaaaaa
    28861 aggcccgggc cagactggcc gagacgggtc gtttaacaat cacacgcgtc ttctccacaa
    28921 aaaatcatta atgttccagt tcgctaaaac gcaatttagt cagtgatggg attatttgat
    28981 ctgccacagt ttatagcgaa atagttactt attctgggca catgaacatt gccgattccc
    29041 ctgtaagtcg ggtaataaca acactcatat aaagaataag gtttttacaa ccaaaaaaga
    29101 aggtcgttat gaatcgtttt attatggcca acagtcagca atgtctgggt tgtcatgctt
    29161 gtgaaatcgc ctgtgtcatg gctcacaatg atgagcaaca tgtcctgagc caacaccatt
    29221 ttcatccccg aattacggtt atcaaacatc agcagcaacg tagtgcagtg acctgtcacc
    29281 attgtgaaga tgcgccctgc gcccgtagct gccctaatgg cgcaatcagc cacgttgatg
    29341 acagcattca ggtcaatcag caaaagtgta ttggctgtaa atcctgcgtg gtggcctgtc
    29401 cgtttggtac gatgcaaatc gtcctgacac ccgtcgcggc aggaaaagta aaagccacgg
    29461 cgcataaatg cgacctttgt gcagggcgcg aaaacggtcc tgcctgtgtc gagaattgcc
    29521 cggcggacgc gctgcaactg gtcactgacg tcgcactctc cggcatggcg aaatcccgcc
    29581 gcttgcgcac cgcacgtcag gagcatcaac cgtggcatgc cagtaccgct gcacaagaaa
    29641 tgccggtaat gagtaaagtc gaacaaatgc aggcaacgcc cgcgcgtggc gagccggata
    29701 aactggcgat tgaagcgcgc aaaaccggtt ttgatgaaat ttatctgcca ttccgtgccg
    29761 atcaggcaca acgggaagcc tcgcgctgcc ttaaatgcgg cgagcacagc gtttgtgaat
    29821 ggacctgccc gctgcataac catattccgc agtggattga actggtgaaa gccggaaaca
    29881 tcgacgccgc cgtcgagctt tctcacctga ccaacaccct gccggaaatt accggacgcg
    29941 tttgtccgca ggaccgtttg tgtgaaggtg cctgtactat tcgcgatgag cacggcgcgg
    30001 taactatcgg caacattgaa cgctacattt cagatcaggc gttggcgaaa ggttggcgtc
    30061 ctgacttaag ccatgtcacc aaagtggaca agcgggtggc gattatcggt gcgggtccgg
    30121 cagggctggc ctgtgcggat gttctgaccc gcaatggcgt ggcggtgacg gtgtacgatc
    30181 gccatccaga aattggtggc ttgctcactt tcggcattcc ttctttcaaa ctggataaat
    30241 ccctgctggc acgccgtcgg gaaatcttca gcgcgatggg gattcacttc gaactcaatt
    30301 gtgaagtggg taaagatgtc tctttggatt cgcttttgga acaatacgac gcggtcttcg
    30361 ttggcgtagg cacttaccgt tccatgaaag cgggtttacc caatgaagat gcgccgggcg
    30421 tttatgacgc gctgccgttc ctcatcgcca acactaaaca ggtgatggga ctcgaagagc
    30481 taccggaaga gccgtttatc aataccgccg gactcaacgt cgtggtactg ggcggcggcg
    30541 acaccgcgat ggactgtgtg cgtaccgcgc tgcgccacgg cgcgagtaac gtcacctgcg
    30601 cttatcgtcg tgatgaagct aacatgccag gctcgaagaa agaggtgaag aacgcccgcg
    30661 aagagggggc caacttcgaa tttaacgtcc agccggtggc gcttgagctg aatgagcaag
    30721 gtcacgtttg cggaattcgt ttcctgcgca cgcgtcttgg cgagccggat gcccaggggc
    30781 gccggcgact tgtaccgatt gagggcagtg aatttgtcat gccagccgat gcggtgatta
    30841 tggtgtttgg cttcaatcca cacgggatgc cgtggctgga gtcgcacggt gtaacggtag
    30901 acaaatgggg ccgcatcatc gcggatgtgg aaagccagta ccgttaccag accaccaatc
    30961 cgaaaatctt cgccggtggc gacgccgtgc gtggtgcgga tctggtggtt accgcaatgg
    31021 cagaaggacg tcatgcggca caggggatta ttgactggct gggggtaaaa tcagtcaaat
    31081 ctcactgata gcctgcgcag acaaacccga cttcacagcg taatataatt gttcatttcg
    31141 cgctgtggag tcggtatgac gcaacaaatc accctcatta aagacaaaat tctctccgat
    31201 aactatttca ccctgcacaa cattacttac gatctcacgc gcaaagatgg cgaagttatc
    31261 cgccataaac gtgaagttta cgatcgcggt aatggtgcga cgatcctcct gtacaacgcg
    31321 aagaaaaaga ccgtggttct gattcgtcag ttccgtgtcg ctacctgggt taatggcaat
    31381 gaaagcgggc agctgattga aacctgcgcc gggctgctgg ataacgacga accggaagtg
    31441 tgcattcgta aagaagcgat tgaagagacg ggctatgaag tgggcgaagt gcgcaaatta
    31501 tttgaactgt atatgtcgcc tggcggtgtg actgagctaa tccacttttt tatcgccgaa
    31561 tacagtgaca atcagcgcgc taacgccggt ggcggtgttg aagatgaaga tattgaagtg
    31621 cttgagctgc cgttcagcca ggcgctggag atgatcaaaa ccggcgagat acgtgacggt
    31681 aagacggtgt tattgcttaa ctatttgcaa acgtcacatt taatggactg aaaaataaca
    31741 ataatatttc gttgtttatt attggctcaa tccgacaaat ctgattgagc cgcgctacct
    31801 gcgcaacgaa gatacgactt gtgctgtttg tttgaacttc tggggtcgta ccgtccatgc
    31861 gctatcgcat tttccttctc ttttttttcg ctttgttgcc gacgtctttg gtgtgggcgg
    31921 cactagcgca acgggcgttt tccgactggc aggtcacctg taataaccaa aacttctgcg
    31981 tggcgcgcaa tacgggcgat cataatggac tggtgatgac cctgagccgc agcgccgggg
    32041 cgcataccga tgccgtttta cgtattgagc gcggcgggtt gaagtcgccg gatgcgtctg
    32101 aaggagagat agcgccacgg ctgctgttag atggtgagcc gttggcgtta agtggtgaca
    32161 agtggcggat ttcaccatgg ttattagtaa ccgatgatac ggcaaccatc accgcgtttt
    32221 tgcagatgat tcaggaaggg aaggcgatca ccttacgcga cggcaatcag accatttctc
    32281 tgagtggctt aaaagcggcg ttgttgttta ttgatgctca gcaaaagcgc gttggcagtg
    32341 aaaccgcgtg gatcaagaaa ggggacgaac cgccgctcag cgtaccgccc gcgcctgcgc
    32401 tgaaagaggt cgcggtggtt aacccaacgc cgacgccact ctcactcgaa gaacgcaacg
    32461 atttgctgga ttatggaaac tggcggatga atggtctgcg ctgctcgctt gatccgttac
    32521 gtcgtgaggt gaatgtcact gcgctgactg atgataaagc gctgatgatg atcagctgtg
    32581 aggcaggggc ctataacacc attgatttgg catggattgt gtcgcgtaaa aagccgctag
    32641 cctcgcgccc ggttcggttg cgtttgccgt tcaacaacgg gcaggagacg aatgaactgg
    32701 aactgatgaa cgcaacattt gatgagaaat cgcgtgaact ggtgacctta gcgaaagggc
    32761 gcggattaag cgattgtggc attcaggcgc gctggcgctt tgacggtcag cgttttcgcc
    32821 tggtgcgtta cgccgcagaa cccacctgcg ataactggca tgggccagac gcctggccca
    32881 cgttgtggat cacccgttaa acaaaatggc ggggacatgt aggccggata agacgctgta
    32941 ggccggataa gacgcgtcag cgtcgcatcc ggcaatcagc atccggcgat cactatcagg
    33001 cacctttcac tcccagcacc ttatgcgctt ttgccacaat attctcggcg gtaaagccaa
    33061 agaacgggaa cagcttatcc gccggagcag attccccata acccgtcatc ccgacaattg
    33121 cccctttcag accaacatac ttgtaccagt aatcggcaat acctgcttcc accgccacgc
    33181 gagccgcaac gttagaaggc aacaccgact cccgatattc ctcatcctgg gcgtcgaaaa
    33241 tatcggtcga gggcagggaa accacgcgaa cattgcgacc ttctcctgct aatttctccg
    33301 cagcttgcag ggtgatttcc atctctgaac cggtggcaat cagaataata tctggcttac
    33361 cgccgctgtc tttcagcaca taaccgccac gagtaatctc tttaacctga tccggcgtac
    33421 gttccacctg ggccagattc tgccttgaga ggatcagtgc cgtcggtccg ttgtggcgct
    33481 caaccgccag cttccagccc accgccgctt ccacctgatc gcacggtcgc caggtgctga
    33541 aatttggcgt taagcgcagg ctggccagtt gctcaacagc ctggtgcgtc ggaccatctt
    33601 cgcccaggcc aattgagtcg tgggtataaa ccataatctg ccgcgctttc atcagtgccg
    33661 ccatccgcgc ggcgttacgg gcgtattcaa caaacatcag gaacgtcgcg gtatacggca
    33721 caaagccgcc gtggtgcgcg atgccgttgg cgatagcggt catgccaaat tcacgcaccc
    33781 cgtagtgaat gtagttgccc gccggatctt ccttcagcga aacagaacct ttccagatgg
    33841 tcaggttgct gggagccaga tccgccgaac cgccgagcag ctccggtagc aaggggccat
    33901 aagcgttaag cgtattttgc gaagccttac gggtggcgat tttcgccgga ttcgcctgca
    33961 actcattgat atacttctga gtcgttttct cccagtcctt cggtaaacca ccgctcatcc
    34021 gacgggtaaa ctcttctgcc agttgcggat gagccttttt ataggcggca aacttctcat
    34081 tccacctctg ctgcgctttt tcgccttttt cgcgggcatc ccaggcgtga taaatatctt
    34141 tagggatctc aaatggcgga tggtgccagc ccagtttttg ccgtgccagc gccacttctt
    34201 cttcccccag cggtgcgccg tgcgcctctt ccttacctgc tttattcggc gatccaaagc
    34261 caatcaccgt acgacagata atcagcgacg gcttatcttt cacgctttgc gcttcaagga
    34321 tcgcttcctt caccgcctgc ggatcgtgac cgtcgatttc atggatcaca tgccagtgat
    34381 aggcttcaaa acgttttgcc gtatcgtcgg taaaccagcc ttctgtttca ccgtcgatgg
    34441 aaataccgtt gtgatcgtaa aaaccaatca gcttgcccag tcccagcgtg cctgccagcg
    34501 aacagacttc gtgggaaata ccttccatca ggcagccgtc gcccataaac acataggtga
    34561 agtgatcgac gatctcatgg tctggctggt taaactgcgc cgccagtgta cgctctgcta
    34621 tcgccagccc gacggcgttc gccaaacctt gtccaagtgg accggtggtg gtttcaacgc
    34681 ctggcgtata accaatctcc gggtggcctg gggttttcga atgcagttga cgaaagttct
    34741 tcagctcttc cagcggcagg tcgtaaccgg tcagatgcag caaactgtag agcagcatcg
    34801 acgcgtgacc gttggaaaga ataaagcggt cgcgatcata ccaggttggg tcggtagggt
    34861 tatgtttaag aaaatcgttc cacagcactt cggcaatatc agccatgccc atcggcgcgc
    34921 cgggatgacc agaattggct ttttgtaccg catccatact gagtgcgcga atcgcattgg
    34981 caaggtcttt tcgggacata taacactccg tggctggttt atagtttggc ggcaagaaga
    35041 tcttccagtt tgcgttgatc aacggcgaac agacgaatgc cttcagacag tttttctacc
    35101 gccatcgcat actgattgtg ctcccaacgg aactccgctt cgctcatggg agctgggcgt
    35161 gggaacgtct gagaaggtgg gattaattta cgtaccactg gcgaaacttt ttcctgcagc
    35221 tccttcagca aattcggtgc gatagtcagt cgatcgcagc cggttaaggc gaggatttgt
    35281 tcggtgcgac ggaagctcgc gcccatcaca atggtttcat agtggtgttg cttatagtag
    35341 tcgtagatat tgcgcaccga tttaacgccc ggatcttctt ccaccacata cgggtccatc
    35401 ggcttgcgtg cctgatacca gtcataaata cgcccgacaa acggcgaaat cagaaaaacg
    35461 cctgcttccg cacaggcccg tgcctgtgca aaagaaaaca gcagcgtcag gttgcagtta
    35521 ataccttctt tttccagctc ttctgccgcg cgaattcctt cccaggtcga agccagcttg
    35581 atcagaatgc gtgatttctc aacgccttgt tgctgataca agtccaccag atggcgcgcc
    35641 ttctcaatac tcttttcttt atcaaaagag aggcgtgcat caacttctgt tgacacgcga
    35701 ccgggtacga ttttgaggat ttcagcaccg aaattgaccg ccagtttgtc acacgctgcg
    35761 accacctgtt gttcctgggt cttgccattt tttttacccc aggcgatagc atcgtctatt
    35821 aaatgctcat attgtgataa tccggcagcc ttgagtaaca gcgaaggatt ggtggtggca
    35881 tcctggggat gataatggcg aatggactca atatcgccgc tgtctgccac gacagtggtg
    35941 aactgtttga tgccgtctaa ctcgttcata ggaaatactc cttgaaaagt aaagtgttag
    36001 atgagtgcgt taattcacac ttctgagaaa tttcgctaaa cgcatcaaaa aagcatagca
    36061 gacaggcatg gtattgctgg attaagcagg taacatcagt gttataggat tattaccaaa
    36121 acattatatt aatggtgaga gtttggactt gctcaaagtc tgtagactcc ggcaaggtaa
    36181 taatgtgcgc cacgttgtgg gcagggggtg ttgcccacac actttatttg tgaacgttac
    36241 gtgaaaggaa caaccaaatg gatgaccagt taaaacaaag tgcacttgat ttccatgaat
    36301 ttccagttcc agggaaaatc caggtttctc caaccaagcc tctggcaacg cagcgcgatc
    36361 tggcgctggc ctactcaccc ggcgttgccg caccttgtct tgaaatcgaa aaagacccgt
    36421 taaaagccta caaatatacc gcccgcggta acctggtggc ggtgatctct aacggtacgg
    36481 cggtgctggg gttaggcaac attggcgcgc tggctggcaa accggtgatg gaaggcaagg
    36541 gcgttctgtt taagaaattc gccgggattg atgtatttga cattgaagtt gatgaactcg
    36601 acccggacaa atttattgaa gttgtcgctg cgctcgaacc gaccttcggc ggaatcaacc
    36661 tcgaagacat taaagcgcca gaatgtttct atattgaaca gaaactgcgc gagcggatga
    36721 atattccggt attccacgac gatcagcacg gcacggcaat catcagcact gccgccatcc
    36781 tcaacggctt gcgcgtggtg gagaaaaaca tctccgacgt gcggatggtg gtttccggcg
    36841 cgggtgccgc agcaatcgcc tgtatgaatc tgctggtagc gctgggtctg caaaaacata
    36901 acatcgtggt ttgcgattca aaaggcgtta tctatcaagg ccgtgagcca aacatggcgg
    36961 aaaccaaagc cgcatatgcg gtggtggatg acggcaaacg taccctcgat gatgtgattg
    37021 aaggtgcgga tattttcctg ggctgttccg gcccgaaagt gctgacccag gaaatggtga
    37081 agaaaatggc tcgggcacca atgattctgg cgctggcgaa tccggaaccg gaaattctgc
    37141 cgccgctggc gaaagaagtg cgtccggatg ccatcatctg caccggtcgt tctgactatc
    37201 caaaccaggt caacaacgtc ctttgcttcc cgttcatctt ccgtggcgcg ctggacgttg
    37261 gcgcaaccgc catcaacgaa gagatgaaac tggcggcggt acgcgcgatt gcagaactgg
    37321 cccatgcgga acagagcgaa gtggtggctt cagcgtatgg cgatcaggat ctgagctttg
    37381 gtccggaata catcattcca aaaccgtttg atccgcgctt gatcgttaag atcgctcctg
    37441 cggtcgcgaa agcggcgatg gagtcgggcg tggcgacgcg tccgattgct gatttcgacg
    37501 tctacatcga caagctgact gagttcgttt acaaaaccaa cctgtttatg aagccgattt
    37561 tctcccaggc tcgcaaagcg ccgaagcgcg tagttctgcc ggaaggggaa gaggcgcgcg
    37621 ttctgcatgc cactcaggaa ctggtaacgc tgggactggc gaaaccgatc cttatcggtc
    37681 gtccgaacgt gatcgaaatg cgcattcaga aactgggctt gcagatcaaa gcgggcgttg
    37741 attttgagat cgtcaataac gaatccgatc cgcgctttaa agagtactgg accgaatact
    37801 tccagatcat gaagcgtcgc ggcgtcactc aggaacaggc gcagcgggcg ctgatcagta
    37861 acccgacagt gatcggcgcg atcatggttc agcgtggcga agccgatgca atgatttgcg
    37921 gtacggtggg tgattatcat gaacatttta gcgtggtgaa aaatgtcttt ggttatcgcg
    37981 atggcgttca caccgcaggt gcaatgaacg cgctgctgct gccgagtggt aacaccttta
    38041 ttgccgatac ctacgtcaat gacgaaccag acgcagaaga gctggcggag atcaccttga
    38101 tggcggcaga aactgtccgt cgtttcggta ttgaaccgcg cgttgctctg ttgtcgcact
    38161 ccaactttgg ttcttctgac tgcccgtcgt cgagcaaaat gcgtcaggcg ctggaactgg
    38221 tcagggaacg tgcgccagaa ctgatgattg atggtgaaat gcacggcgat gcggcgctgg
    38281 tggaagcgat tcgcaacgac cgtatgccgg acagcccttt gaaaggttct gccaatattc
    38341 tggtgatgcc gaacatggaa gctgcccgca ttagttacaa cttactgcgc gtttccagct
    38401 cggaaggtgt gactgtcggt ccggtgctga tgggcgtggc gaaaccggtt cacgtgttaa
    38461 cgccgatcgc atcggtgcgt cgtatcgtca acatggtggc gctggccgtg gttgaagcgc
    38521 aaacccaacc gctgtaattc ttttttaact ctcacgctta ccctgaatat tcagggtaag
    38581 cggtttggct gcaatatatt agtaaagctt attactgagt ttgcgaataa taaaaaaagc
    38641 agtctatata atatctcgat attattttat ttatattcat gcgttgcata tgaaagttta
    38701 tgcaccacag cgaatatctc tccattctta gtgatctacc tcacctttta aacgcgcttg
    38761 ccgaattttg ttatttactc tgacgaaaaa ttgtcacgat acacgaaagt ttttcacagg
    38821 cggcgactca tggataaaga acgcatcatt caggaatttg tgcccggcaa acaggtcaca
    38881 ctggcgcatc tcatcgcgca tcccggtgag gaactggcga agaaaatcgg cgttccggat
    38941 gcgggcgtaa ttggcattat gacgctaact cccggcgaaa cggcgatgat cgtcggtgat
    39001 ttagcgctga aagccgctga tgtgcatatc ggttttctcg accgcttcag cggtgcattg
    39061 gtgatttatg gctcggtggg cgcagtagaa gaagcgttat cacaaaccgt cagcggttta
    39121 ggtcgcttat taaattatac gctttgtgaa atgaccaaaa gttaatacga ggcattcatg
    39181 aaacgaattg cgtttgtggg tagcgtcggg gcaggaaaaa caacgttatt taatgcgttg
    39241 caggggaatt ataccctcgc cagaaaaaca caggccgtgg aatttaatga taagggcgat
    39301 attgatacgc cgggtgaata ttttagccat ccccgctggt atcacgcctt aattaccacg
    39361 ctacaggatg tagacatgct gatctatgtc catggcgcaa atgacccgga aagtcggttg
    39421 cctgccgggt tgctggatat tggcgtcagc aaacggcaaa tcgccgtcat cagtaaaacg
    39481 gacatgccag atgctgacgt cgccgcaacg cgaaagttat tgcttgagac gggtttcgaa
    39541 gagccgattt tcgagctaaa cagccacgac ccgcaaagcg tgcagcaact ggtggattat
    39601 ctggcatctc tcaccaaaca ggaggaagca ggtgaaaaaa ctcatcacag cgaatgatat
    39661 tcgtgaagca cacgcgcgcg gcgaacaggc aatgtccgtg gttctgcgcg ccagcattat
    39721 taccccggaa gcccgtaaag tggcggatct actgggtttt accatcaccg aatgtgatga
    39781 atcgatcccg gtaacggcgt ccgtgcccgc cagcgtgcca gccgacaaaa ccgaaagcca
    39841 gcgcatccgc gaaaccatca ttgcccagct gccggaaggc cagtttaccg aaagcctggt
    39901 cgcacagctg atggaaaaag tgatgaagga aaaacagtcg ctggagcagg gcgcaatgca
    39961 gccgagcttc aaatcggtga ccggcaaagg cggcatcaaa gtgattgatg gcagcagcgt
    40021 caagtttggt cgttttgatg gcgcacaacc gcattgcgtg ggcttgactg atcttgtgac
    40081 cggagacgac ggcagcagca tggccgccgg ttttatgcag tgggagaacg cattcttccc
    40141 gtggaccctg aactacgacg aaatcgacat ggtgctggaa ggggagctcc atgttcgcca
    40201 cgaaggcgaa accatgatcg ccaaagcagg cgacgtgatg tttatcccga aaggctccag
    40261 catcgaattt ggtacgacat ccagcgtgaa attcctgtat gtcgcctggc cggctaactg
    40321 gcaatcccta tgaaagattt catcaccgaa gcatggctaa gagcgaacca tacgctcagc
    40381 gaaggcgcag agatccatct ccctgcggac agtcgcctga cgccctctgc ccgggagttg
    40441 ctggaaagcc gccatctgcg catcaagttt attgacgagc agggccgcct gtttgttgac
    40501 gatgaacagc agcagccgca gcccgttcat gggctgacca gtagcgatga acatccgcag
    40561 gcgtgctgcg aactgtgccg ccagccggtg gcaaaaaagc cggatacgct gactcacctg
    40621 tcggcggaaa aaatggtcgc caaaagcgat ccgcgtctgg gttttcgcgc ggttctcgac
    40681 agcaccattg cgctggcagt gtggctgcaa attgaactgg cggaaccgtg gcagccgtgg
    40741 ctggcggata tccgttcgcg tcttggcaac attatgcgcg ccgatgcgct gggggaaccg
    40801 ctgggctgcc aggcgattgt cgggctttct gacgaagatc tgcatcgact ttctcaccag
    40861 ccgctgcgct atctcgacca cgatcatctg gtgccggaag ccagccacgg gcgcgatgcg
    40921 gcgttgctca atctgctacg taccaaagtg cgggaaaccg aaaccgtagc ggcgcaggtg
    40981 tttatcaccc gcagctttga agtattacgc ccggacatct tgcaggcgct gaaccgcctt
    41041 tcgagcacgg tctacgtaat gatgatcctg agcgtaacga agccgccgct aacggtgaag
    41101 cagatccaac aacgactggg ggaaacgcaa tgattattga acgttgtcgt gaactggcgt
    41161 tgcgagcgcc cgccagagtg gtttttccgg atgcgttaga tcaacgtgtg ctgaaagctg
    41221 cgcaatattt acatcaacaa ggtctggcaa cgcccattct ggtcgctaat ccgtttgaac
    41281 ttcgtcagtt tgcgctcagt cacggcgtgg cgatggacgg gctacaggtg attgatccgc
    41341 atggcaacct cgcaatgcgg gaagaatttg ctcatcgctg gctggcccgc gcgggcgaaa
    41401 aaacgccgcc ggatgcgctg gaaaaactta ccgatccgct gatgttcgcc gcagcaatgg
    41461 tcagcgccgg taaagcggat gtctgtatcg cgggcaacct ctcttccacg gcgaatgtgc
    41521 tgcgtgccgg attacgtatt atcggcttgc agccaggctg taaaacgctc tcatccattt
    41581 tcctgatgct gccacagtac agcggtccgg cgttgggctt tgccgattgc agcgtggtgc
    41641 cacagccgac ggcggcgcaa ctggcggata tcgcgcttgc cagtgccgaa acctggcgcg
    41701 ccctcaccgg agaagagccg cgcgtggcga tgctgtcgtt ttccagtaac ggtagcgccc
    41761 gtcacccctg cgttgccaac gtccagcagg cgacagaaat cgtccgtgag cgcgcaccaa
    41821 agctggtggt tgatggcgag ttgcagtttg acgccgcctt cgtgccggaa gtggcggcgc
    41881 aaaaagcgcc tgccagcccg ctacagggca aagccaatgt gatggttttt ccgtcgctgg
    41941 aagccggaaa tattggctac aaaatcgcac aacgactcgg cggatatcgt gccgtcgggc
    42001 cattgataca aggacttgcc gcgccgatgc acgatctctc tcgtggttgt agcgtgcagg
    42061 aaattatcga gctggcgctg gtggcagctg tgccgcgtca gacagaagtg aaccgcgaaa
    42121 gcagtttaca aacactggtt gaatgaacgg tcccgttctg gaccctgata aagaggaaag
    42181 cacaatggaa gcattaggaa tgatcgaaac ccggggcctg gttgcactga ttgaggcttc
    42241 tgacgcgatg gttaaagctg cgcgcgtcaa gctggttggt gttaagcaga ttggcggcgg
    42301 cctgtgtact gccatggttc gtggtgatgt tgcagcctgt aaagccgcga ccgatgcagg
    42361 tgccgcagcg gcacagcgta tcggcgagct ggtatctgta cacgttatcc cgcgtccgca
    42421 cggcgacctg gaagaagtct tcccgatcgg cctgaaaggc gatagcagca acctgtaatc
    42481 accctgtgtg acgtcgattc gggcgtcacg ctctctcctt tttcatttta ccttctgcgg
    42541 agggtagggg gagcttttac ctgaaatacg ccacggaggc gggtatgaaa ctggcagtcg
    42601 tcactggaca aattgtttgt accgtacgcc atcacggact ggcgcatgac aaattgctga
    42661 tggtggaaat gattgatcca caaggtaatc ctgacgggca atgcgccgtc gccatcgaca
    42721 atattggcgc gggaaccggg gagtgggtgt tactggtgag tggcagttcc gcccgccagg
    42781 cgcataaaag cgaaacgtca ccggtcgatc tgtgcgtgat tggcattgtc gatgaggtgg
    42841 tgtctggcgg tcaggtaatt ttccacaaat aaggcagaac atcatgaatc aacaggatat
    42901 tgaacaggtg gtgaaagcgg tactgctgaa aatgcaaagc agtgacacgc cgcccgccgc
    42961 cgttcatgag atgggcgttt tcgcgtccct ggatgacgcc gttgcggcag ccaaagtcgc
    43021 ccagcaaggg ttaaaaagcg tggcaatgcg ccagttagcc attgctgcca ttcgtgaggc
    43081 aggcgaaaaa cacgccagag atttagcgga acttgccgtc agtgaaaccg gcatgggccg
    43141 cgttgaagat aaatttgcca aaaacgtcgc tcaggcgcgc ggcacgccag gcgttgagtg
    43201 tctctctccg caggtgctga ccggcgacaa cggcctgact ttaattgaaa acgcgccctg
    43261 gggcgtggtg gcttcggtaa ctccgtccac taacccggcg gcaaccgtaa ttaataacgc
    43321 catcagcctg attgccgcgg gcaacagcgt catttttgcc ccgcatccgg cggcgaaaaa
    43381 agtctcccag cgggcgatca cgctgctcaa ccaggcgatt gttgccgcag gcgggccgga
    43441 aaacttactg gttactgtgg caaatccgga tatcgaaacc gcgcagcgtt tgttcaagtt
    43501 tccgggcatc ggccttctgg tggtgaccgg cggcgaagcg gtagtagaag cggcgcgtaa
    43561 acacaccaat aaacgtctga ttgccgcagg cgctggcaac ccgccggtag tggtggatga
    43621 aaccgccgac ctcgcccgtg ccgctcagtc catcgtcaaa ggcgcttctt tcgataacaa
    43681 catcatttgt gccgacgaaa aggtactgat tgttgttgat agcgtagccg atgaactgat
    43741 gcgtctgatg gaaggccagc acgcggtgaa actgaccgca gaacaggcgc agcagctgca
    43801 accggtgttg ctgaaaaata tcgacgagcg cggaaaaggc accgtcagcc gtgactgggt
    43861 tggtcgcgat gcggcgaaaa tcgcggcggc aatcggcctg aacgtgccgc aagaaacgcg
    43921 tttgctgttt gtggaaacca ctgcagaaca tccgtttgcc gtgaccgaac tgatgatgcc
    43981 ggtgctgccc gtcgtgcgcg tcgccaacgt ggcggatgcc attgcgctgg cggtgaaact
    44041 ggaaggcggt tgccaccaca cggcggcaat gcactcgcgc aacatcgaaa acatgaacca
    44101 gatggcgaat gctattgata ccagcatttt cgttaagaac ggaccgcgca ttgccgggct
    44161 ggggctgggc ggggaaggct ggaccaccat gaccatcacc acgccaaccg gtgaaggggt
    44221 aactagcgtg cgtacgtttg tccgtctgcg tcgctgtgta ttagtcgatg cgtttcgcat
    44281 tgtttaagga gtaacagatg gcgcacgacg aacaatggct caccccacgt ctgcaaacgg
    44341 cggcaacgct gtgtaaccag acgcccgccg cgacggaatc tccgctgtgg ctgggggtgg
    44401 atctgggcac ctgcgatgtg gtgtcgatgg ttgtcgaccg cgacggtcag tcggtggcgg
    44461 tatgcctcga ctgggccgat gtggtccgcg acggcatcgt ctgggatttc ttcggcgctg
    44521 tcaccattgt tcgtcgccat ctcgacacgc tcgagcagca attcggtcgc cgttttagcc
    44581 atgcggcgac ttcatttcct ccaggtacgg acccgcgtat ttccatcaac gtgctggagt
    44641 ctgctggact ggaagtgagc catgtgctgg atgagccaac cgcagttgcg gatctgctgc
    44701 aactggacaa cgccggtgta gtggatatcg gcggcggcac taccggcatt gccatcgtga
    44761 aaaagggcaa ggtgacgtac tcggcggatg aagcaaccgg cggacatcac atctctctga
    44821 ctctcgccgg aaatcgccgt atttcgctgg aagaggcgga gcagtacaag cgcggtcacg
    44881 gtgaagagat ttggcctacg gtgaaaccgg tgtacgaaaa aatggtggac attgtcgccc
    44941 ggcatattga agggcagggc attactgatt tatggctggc gggcggctct tgtatgcaac
    45001 cgggcgtggc ggaactgttt cgcaaacaat tcccggcgtt acaggtgcat ttaccgcagc
    45061 acagtttgtt tatgaccccg ctggcgatcg ccagtagtgg gcgagagaaa gcggaggggc
    45121 tatatgcaaa atgaattgca gaccgcgctc tttcaggcgt tcgataccct gaatctgcaa
    45181 cgggtaaaaa catttagcgt tccaccggtg acgctttgcg gtccgggctc ggtgagcagt
    45241 tgcggacagc aagcgcaaac gcgtgggctg aaacatctgt tcgtgatggc agacagcttt
    45301 ttgcatcagg cagggatgac cgccgggctg acgcgcagcc tggctgttaa aggcatcgcc
    45361 atgacgctct ggccatgtcc ggtgggcgaa ccgtgcatca ccgacgtgtg tgcagccgtg
    45421 gcgcagttgc gtgagtcagg ctgtgatggg gtgatcgcat ttggcggcgg ctcggtgctg
    45481 gatgcggcga aagccgtggc gttgctggtg acgaacccgg atagcacgct ggcagagatg
    45541 tcagaaacca gcgttctgca accgcgcttg ccgctgattg ccattccaac gaccgccgga
    45601 accggctctg aaaccaccaa tgtaacggtg attatcgacg cggtgagcgg gcgcaagcag
    45661 gtgttagccc atgcctcgct gatgccggat gtggcgattc tcgacgccgc gttgaccgaa
    45721 ggtgtgccgt cgcatgtcac ggcgatgacc ggcattgatg cgttaaccca tgccattgaa
    45781 gcatacagcg ccctgaacgc tacaccgttt accgacagtc tggcgattgg tgccattgcg
    45841 atgattggca aatcgctgcc gaaagcggtg ggctacggtc acgaccttgc cgcgcgcgag
    45901 agcatgttgc tggcttcatg tatggcggga atggcgtttt ccagtgcggg tcttgggttg
    45961 tgccacgcga tggcgcatca gccgggcgcg gcgctgcata ttccacacgg tctggcgaac
    46021 gccatgttgc tgccaacggt gatggaattt aaccggatgg tttgtcgtga acgctttagt
    46081 cagattggtc gggcactgcg aactaaaaaa tccgacgatc gtgacgctat taacgcggta
    46141 agtgagctga ttgcggaagt tgggattggt aaacgactgg gcgatgttgg tgcgacatct
    46201 gcgcattacg gcgcatgggc gcaggccgcg ctggaagata tttgtctgcg cagtaacccg
    46261 cgtaccgcca gcctggaaca gattgtcgga ctgtacgcgg cggcgcaata aatgccggat
    46321 gcgacgcttg ccgcgtctta tccagcctac gggattgcac atgtagggcg gataaggcgt
    46381 tcacgccgca tccgccagtg gcgcggtgca attgccggat gcgatgcttg ccgcgtctta
    46441 tccggcctac gggattgcac atgtagggcg gataaggcgt tcacgccgca tccgccagtg
    46501 gcgcggtgca attgccggat gcgacgcttg ccgcgtctga tccggccttc gggattgcac
    46561 atgtagggcg gataaggcgt ttacgccgca tccgccaata aataacaact aacagggagt
    46621 aaaggcgatg ggaattaacg aaatcatcat gtacatcatg atgttcttta tgctgatagc
    46681 tgccgtagac aggatcctgt cgcagttcgg cggttctgct cgtttcctcg gtaagttcgg
    46741 taaaagtatc gaaggatcag gcggtcagtt cgaagaaggc tttatggcaa tgggcgctct
    46801 gggtctggcg atggtcggta tgaccgcgct ggcaccggta ctggctcacg tactcgggcc
    46861 ggtaattatc ccggtttacg aaatgctcgg cgcaaacccg tcaatgttcg ccggaacgct
    46921 gctggcgtgc gatatgggcg gcttcttcct cgccaaagag ctggcgggcg gcgacgtagc
    46981 agcgtggcta tactctgggt taattcttgg gtcgatgatg gggccaacga ttgtgttttc
    47041 cattccggtg gcgctcggca ttatcgaacc ttctgaccgt cgttatctgg cgctcggcgt
    47101 gctggcgggc attgtgacca ttccgattgg ctgtattgct ggtggtctgg ttgctatgta
    47161 ctccggtgtg cagatcaacg gtcagccggt ggaattcact ttcgccctga tcctgatgaa
    47221 catgatcccg gtgatcattg ttgcgattct ggtggcgctg gggctgaaat tcatcccgga
    47281 aaaaatgatc aacggcttcc agatcttcgc caaattcctc gttgcattga tcaccctcgg
    47341 tcttgctgcc gcggtagtta agtttctgct tggctgggaa ctgatccccg gtctggaccc
    47401 tatctttatg gcccctggcg ataaacccgg tgaagtgatg cgcgccattg aagttatcgg
    47461 ttctatctcc tgcgttctgt taggggcgta tccaatggtg ctgctgctga cccgctggtt
    47521 tgaaaaaccg ctgatgagcg tcggtaaagt gctgaatatg aacaacatcg cggcagccgg
    47581 catggtggca acgcttgcca acaacatccc gatgttcggc atgatgaagc agatggatac
    47641 ccgcggcaaa gtcatcaact gcgccttcgc cgtttccgct gctttcgccc tgggcgacca
    47701 cttaggcttc gccgctgcca acatgaacgc catgatcttc ccgatgattg tcggcaagtt
    47761 gatcggcggc gtaacggcga ttggcgtggc gatgatgctg gtgccaaaag aagacgcgac
    47821 cacggctaaa accgaagcgg aggcacaatc gtgaacactc gccagctatt gagcgtcggt
    47881 atcgatatcg gcaccaccac cacccaggtg attttctccc acctggagct ggttaaccgt
    47941 gcggcggtgt cgcaggtgcc gcgctacgaa ttcattaaac gcgaaattag ctggcaaagc
    48001 ccggtgttct ttacccctgt cgataaacag ggtggtttaa aagaagcgga actgaaaacc
    48061 ttaatcctcg agcaatatca ggctgcgggc attgcgccgg aaagcgttga ttctggtgcc
    48121 atcatcatca ccggtgaaag cgcgaaaacc cgcaatgctc gtccggcggt gatggtgctc
    48181 tcccagtcgc tgggcgattt tgtcgttgcc agcgccgggc cgcacctcga atccgtcatt
    48241 gccggtcacg gtgccggggc acaaaccctt tctgaacaac ggctgtgtcg ggtactgaat
    48301 attgacatcg gcggtggcac tgcgaactac gccctgttcg atgccggaaa aatcagcggc
    48361 actgcctgcc tcaacgtcgg tggtcgcctg ctggaaaccg acagccaggg gcgcgtggtt
    48421 tacgctcata aaccggggca gatgattgtg gatgagtgct tcggcgcagg caccgacgcc
    48481 cgttcgctga ccggcgcgca gctggtgcag gttacccggc ggatggcaga actgattgtc
    48541 gaagtgattg acggaacgct ctcgccgctc gcgcaggcat tgatgcaaac cggtttgctg
    48601 cccgcaggtg ttacgcccga aatcattacg ctttctggcg gcgtgggcga atgttatcgc
    48661 caccagcccg ccgacccgtt ctgttttgcc gatattggcc cgctgctggc aacggcgctg
    48721 catgaccatc cgcgcctgcg tgagatgaac gtacagtttc cggcgcaaac cgtgcgcgcc
    48781 acggtgattg gcgcgggcgc gcataccctt tcgctctctg gcagcacaat ctggcttgag
    48841 ggcgtacaac tgccgctgcg caatttgccg gtggcgatcc cgattgatga aacggatctg
    48901 gtgagtgcct ggcaacaggc gctgcttcag ctggatcttg atcccaaaac tgacgcgtac
    48961 gtgctggcgc ttcccgcctc gctgcctgtg cgttacgccg cggtactgac ggtcatcaac
    49021 gcgctggtcg atttcgtcgc gcgttttcca aatccgcatc ccctgctggt ggtggccggg
    49081 caggactttg gtaaagctct gggcatgttg ttgcgcccac agctacaaca actcccgttg
    49141 gcagtcattg acgaagtgat tgtccgcgcg ggggactata tcgacattgg tacgcctctt
    49201 tttggcggat cggttgtgcc ggtgacggtg aaatcactcg catttccttc ctgagggaac
    49261 gacttatgaa actaaagacc acattgttcg gcaatgtata tcagtttaag gatgtaaaag
    49321 aggtgctggc taaagccaac gaactgcgtt cgggggatgt gctggcgggc gttgcagcgg
    49381 caagctcaca ggagcgcgtg gcggcaaagc aggtgttgtc ggaaatgacc gtagcggaca
    49441 tccgcaataa tccggtgatt gcctatgaag atgactgcgt gacgcggctg attcaggacg
    49501 atgttaacga aacggcctac aaccagatta aaaacaggag catcagcgaa ctgcgtgagt
    49561 atgtgctgag cgatgaaacc agcgtggacg acattgcctt tacccgcaaa gggctgacct
    49621 cggaagtggt cgcggcggta gcgaagattt gctccaacgc ggacctgatc tacggcgcga
    49681 agaaaatgcc ggtaatcaaa aaggccaata ccaccatcgg tattccgggc accttcagcg
    49741 cccgtttgca gccaaatgac acccgtgacg acgtgcaaag tatcgctgca caaatctacg
    49801 aagggctttc cttcggggtg ggcgatgcgg tgatcggcgt taacccggta actgacgacg
    49861 tggaaaactt aagccgcgtg ttggacacca tttacggcgt gatcgacaaa ttcaacatcc
    49921 caactcaggg ctgcgtactg gcgcacgtca ccacccagat cgaagcgatt cgtcgcggcg
    49981 cgcctggcgg actgattttc cagagtatct gtggcagcga aaaagggctg aaagagtttg
    50041 gcgtggaact ggcgatgctc gacgaagcgc gcgcagtggg tgctgagttc aaccgtatcg
    50101 ccggggaaaa ctgcctctac ttcgaaaccg ggcaaggttc ggcgctgtct gctggcgcta
    50161 acttcggcgc agactaggtg acgatggaag cacgtaacta tgggctggcg cgtcattacg
    50221 atccgtttat cgtcaacacc gtggtcggct ttattgggcc ggagtatctc tacaacgacc
    50281 gccagattat ccgtgctggc ttagaagatc actttatggg caagctgagc ggcatctcta
    50341 tgggctgtga ctgctgttac accaaccacg ctgatgctga ccagaacctc aacgaaaacc
    50401 tgatgatcct gctcgccacc gcaggctgca actacatcat ggggatgccg ctgggcgatg
    50461 acatcatgct caactatcag accaccgcat tccacgacac tgccactgtg cgtcagttac
    50521 tcaacctgcg cccgtcaccg gagtttgaac gctggctgga aagcatgggc attatggcaa
    50581 acggtcgcct gaccaaacgg gcgggcgatc cgtcactgtt cttctgatga cgcggagata
    50641 acacatcatg gatcaaaaac agattgaaga aattgtacgc agcgtgatgg cgtcaatggg
    50701 acaagcggcc cccgcgccgt cagaagcaaa gtgcgccacc actaactgtg cggcaccggt
    50761 gacctcggaa agctgcgcgc tggatttagg ttctgcggaa gcaaaagcgt ggattggtgt
    50821 tgaaaacccg catcgcgcag acgtattaac agaactgcgc cgcagcaccg tggcccgcgt
    50881 ttgtaccggt cgtaccggtc cgcgtccacg tacgcaggcg ctgctgcgtt tcctggccga
    50941 tcactctcgt tcgaaagata ccgtactgaa agaagtgccg gaagagtggg tgaaagcgca
    51001 gggcttgctg gaagtacgct ccgaaattag cgacaaaaat ctctacctga ctcgcccgga
    51061 tatgggccgc cgcctgtgtg cagaagctgt tgaagcgctg aaagcacagt gtgttgctaa
    51121 tccggacgta caggtcgtta tttctgatgg cctgtcaact gatgccatca ccgtgaacta
    51181 cgaagagatc ctgccgccgc tgatggcggg cctgaaacag gccgggctga aagtcggcac
    51241 gccattcttt gttcgttatg gtcgcgtgaa gattgaagat cagatcggtg agatcctcgg
    51301 cgcgaaagtg gtgatcctgc tggtggggga acgtccgggg ttgggtcagt cagaaagcct
    51361 ctcctgttac gccgtttact cgccgcgtat ggcgaccacc gttgaggccg atcgcacctg
    51421 catctctaac attcaccagg gcggcacgcc gccggttgaa gccgcagccg tcattgtgga
    51481 tttggcgaaa cgtatgctgg agcagaaagc atccggcatc aacatgaccc gataaggagg
    51541 catcatgcca gctttagatt tgattcgacc gtcggtaacc gctatgcggg tgattgcctc
    51601 cgttaacgct gaatttgcgc gtgaactaaa attgccgccg catattcgta gcctcgggct
    51661 gatttctgct gattccgatg acgtcaccta cattgcggct gacgaagcga ccaagcaggc
    51721 gatggtcgaa gtggtatatg gccgctcgct gtatgcggga gcagcacacg gaccgtcacc
    51781 gactgccggt gaagtgctga ttatgcttgg tgggccaaac ccggcggaag tgcgtgctgg
    51841 tctggatgcg atggttgcgc atattgaaaa tggcgctgct ttccagtggg ctaatgacgc
    51901 agaaaacacc gcattcctgg cacatgtagt ttcgcgtacc ggttcttatc tttcatcaac
    51961 cgccgggatc acgcttggcg atccgatggc gtatctggtg gcaccgccgc tggaagcgac
    52021 ctacggcatt gatgcagcgt tgaaatctgc cgacgtacag ctggtgacct atgtcccgcc
    52081 accgtctgaa accaactact cggcagcatt tttaaccggt agccaggccg cgtgtaaagc
    52141 agcctgtaac gcctttaccg atgcagtgct ggaaatcgcg cgtaatccaa tccagcgtgc
    52201 gtaacggagg ttgccgatga tcaatgcact gggattgctg gaagtggacg gcatggtcgc
    52261 cgcgatagat gcagcggatg ccatgctcaa agcagctaac gttcgtctgc tcagtcacga
    52321 agtgcttgac cctggtcgct taacgctggt ggtggaaggc gatctggcgg cgtgtcgtgc
    52381 ggcgctggac gcaggttgtg ctgccgcgat gcgtaccggg cgtgtcatca gccgcaagga
    52441 gatcggtcgg ccagacgatg acacccagtg gctggttact ggctttaacc gccagccgaa
    52501 gcaacccgta aaggaacccg acgcgccagt tatcgtcgcg gaatctgctg acgagttgtt
    52561 ggcgctgtta acatcagtac gtcagggaat gacggcagga gaagtggctg cccactttgg
    52621 ctggccgctg gaaaaagcca gaaatgcgct cgaacagctc ttttctgccg ggacgttacg
    52681 taaacgcagt agtcgttatc gtctcaagcc ccattaacct gtcggaggtg ccgggtgtcc
    52741 tacaacaccc ggcattaaca tcatgaaaaa gacccgtaca gccaatttgc accatcttta
    52801 tcatgaaccc ttacccgaaa acctgaagct cacgccgaag gtcgaagtgg ataatgttca
    52861 tcaacgacag acaacggatg tctatgaaca tgctttgacg attaccgcct ggcagcagat
    52921 ttacgatcag ctgcatccgg gcaagtttca tggtgaattt acggaaattc tgctcgatga
    52981 tattcaggtt tttcgtgaat acaccggtct ggcgctgcgt cagtcgtgcc tggtctggcc
    53041 gaactcgttc tggtttggca ttccggcgac gcgcggtgag cagggattta tcggttcgca
    53101 atgtctggga agcgcagaaa tagcgacgcg ccccggtgga accgagtttg aattaagtac
    53161 gccggacgat tatacgatcc tgggcgtagt gctgtctgaa gatgtcatca cccgtcaggc
    53221 taactttttg cacaatccgg atcgggtatt acatatgctg cgtagccagt cggcgctgga
    53281 agtgaaagag cagcataaag ccgcgctgtg gggctttgtc caacaggcgc tggcgacgtt
    53341 ttgcgagaac ccggaaaatc tccatcagcc tgcagtgcga aaagtgctgg gggataattt
    53401 gctaatggcg atgggggcga tgctggaaga cgcgcaacca atggtgacgg cggaaagcat
    53461 cagtcatcag agttaccgta gattactttc ccgcgcccgt gaatatgtgc tggaaaatat
    53521 gtctgagctg gtgacggtgc tggacttgtg taatcaactg catgtcagtc gccgcacgct
    53581 acaaaacgcg tttcacgcta ttttaggcat tggcccaaac gcgtggctga aacgcattcg
    53641 cctgaacgcc gtacgccgcg aactgataag cccgtggtcg caaagcacaa cggtaaaaga
    53701 cgccgccatg cagtggggat tctggcatct ggggcaattt gccacggatt accagcagct
    53761 gtttgccgag aagccgtcgt tgacgttgca tcagcggatg cgggaatggg ggtgagggag
    53821 ttacacccaa tccctgacct taataaactc acttaacgcc gcttctgggc tgccatcttt
    53881 tggctgataa tcatactccc agcgtaccag tggcggcatt gacatcagga tcgactcggt
    53941 gcgcccgcca gtttgcaggc caaacagagt gccgcgatcc cagaccagat tgaactcgac
    54001 ataacgaccg cgacggtaga gctgaaaatt gcgctcgcgc tcgccgtagg ccatcgcttt
    54061 acgtcgctct acaattggta aataagcgtc ggtgtagcct ttgcctaccg cctgcataaa
    54121 ggcaaaacag tggtcgaaat ctggcgtgtt cagatcatca aagaacagcc cgccaatacc
    54181 gcgctgttcg ttgcgatgtt tgaggtagaa gtaatcgtcg caccactttt tgtaacgggg
    54241 ataaacgtct tcaccaaatg gcaggcacag gtcacgggcg gtgcggtgcc agtgaatggc
    54301 gtcttcttca aaaccataga aaggggttaa atcgaagccg ccgccaaacc accagacggg
    54361 ctctgcaccc ggtttttcgg caataaaaaa acgcacattc gcgtggctgg tgggaacata
    54421 cgggttatgc ggatgcacta ccagtgaaac gcccatcgcc tcgaaactgc gcccggcaag
    54481 ttccgggcga tgagcggtgg cggaagcagg catcgcctcg ccgtggacgt gagaaaagtt
    54541 gacgcctgcc tgttcgaaaa caccaccatt acgcaacacc cgactacgcc cgccgccgcc
    54601 agcttcgcgc tgccaactat cttcgacaaa ttctgcgcca tcgacggcgg tcagctgctg
    54661 acaaatcgta tcctgaaggt tgagcagaaa ctgtttaacc tggtgtgcgt cgggtttcat
    54721 aacttatcgc tttttcgaat gtgctttctg gttgtcgaac cagtggaaat aactgatcac
    54781 gccttcagca atcgctgtgg cgattttctg acgaaacgcc gccgtgccta acagccgctc
    54841 ttcttccggg ttggtgataa acgaggtttc caccagcacc gaaggaaccg acggcgattt
    54901 caacaccaca aacgcggctt gttcggtgtt gcggctgtgc agtttatgca ccggcttaat
    54961 ctttttcaga atatgcgagc caagcgtcag gctgttttta atagtatcgg tttgcaccag
    55021 atcaaacagc acttgctgca ataggtgatc cttgtcagtc gcctttttac cggcaacttc
    55081 atcggcgcgg ttttcgcgtt cagacaggta tttcgccatt gcgctacttg ccccacggtt
    55141 agagagggca aataccgaag caccggcagc tttcgggttg gtaaagccat cggcatgaat
    55201 tgacataaac agatctgcgc catgtttatg ggcgatttca acgcgatcgt aaagtgggat
    55261 aaacgtatcg ccagaacgcg ttaaacgcgc atcaatccca tgattacgca aaatggaacg
    55321 gacgttttta gcaatcgcca gcaccacatg tttttctttc gaaccgttgc gtccgatcgc
    55381 tccggtatca attccgccgt ggcctggatc gagaacaacg acacgtttgc cgccagattt
    55441 tttggctttc ggcttgctgt gtccgttgct ggtttttaaa ggttcgtctt tggcgatggc
    55501 ttgcgacatt cctgacaacg tcagggcagc caaaccggct ttcagcacct ggcggcgcga
    55561 agtgagtgtt tttagtggtt taaaagtgct catacggcct gagttgtaat aataaagttc
    55621 cagatgttat atcgtatcgc gtaatccgtt acgacggttt gatgttgaga attgttttac
    55681 ttttcatttc aatagttgac aactccccat tatgccactt tttcaaccga ttttcgtcag
    55741 atattggcta attcgaccgt tcacgccata atcagccttt taacccgcaa aaaggtaacg
    55801 aataccatgg agatacgcgt atttcgccag gaagatttcg aagaggtcat caccctttgg
    55861 gagcgttgcg acttgctgcg tccgtggaac gatccggaaa tggacatcga gcgtaagatg
    55921 aaccatgacg tcagtttgtt tttggtcgct gaggtaaacg gtgaagtggt cggaacggtg
    55981 atgggcggtt atgacgggca tcgcgggtct gcttattatc ttggcgtgca tccagagttt
    56041 cgtgggcgtg ggattgccaa tgcgttgctt aatcggctgg agaaaaagct gattgctcgt
    56101 ggctgcccga aaattcagat caatgtgccg gaagataacg acatggtgct cggaatgtat
    56161 gaacgcctgg gatatgaaca cgccgacgtg ctgagtctgg gtaagcgttt gattgaagat
    56221 gaagagtact gagttccatc ctgtccatta tgatgcgcac ggacgcctgc gtttaccctt
    56281 gctcttctgg cttgtgctat tgcttcaggc gcgaacctgg gtgctgtttg tcattgccgg
    56341 tgcgtcgcgc gagcagggta ctgcgctgct aaatctgttt tatcccgatc acgataattt
    56401 ctggctgggg ctgattccag gcattccggc ggtgctggcg tttttgttga gcggtcggcg
    56461 ggctacattt cctcgcactt ggcatgtgct ctattttctg ttgctgttgg cgcaggtggt
    56521 tttactttgc tggcaaccgt ggctgtggct gaacggtgaa tccgttagcg gtatcggtct
    56581 ggcgctggta gtggcggata ttgtggcgtt aatctggctg ctgaccaatc gacgtttacg
    56641 cgcttgtttt aatgaagaaa aagaataacg gcactttttg gtgaatttgc actccaagca
    56701 acgttattga ataaccaaag gaagtgacat gaaatcgctg cgtttaatgt tatgcgctat
    56761 gccgttgatg ctgaccggct gttccacgat gtcgtcagtt aactggtcgg ccgctaaccc
    56821 gtggaactgg tttggatcgt ccaccaaagt gagcgagcag ggcgtgggtg aattaacggc
    56881 gtccacacca ctgcaagaac aagccattgc tgatgcgctt gatggcgatt atcgcctgcg
    56941 cagcggaatg aaaaccgcga acggcaacgt ggtgcgcttt tttgaagtga tgaaaggcga
    57001 caacgtggcg atggtgatta acggcgatca aggcacgatc agtcgcattg acgtgctgga
    57061 tagcgatatt cctgctgaca ccggtgttaa aatcggtaca ccgtttagcg acctttacag
    57121 caaagcattt ggcaattgcc aaaaagctga tggtgatgat aatcgtgccg tcgaatgtaa
    57181 agccgaaggc agtcaacata ttagctacca gttcagcggg gaatggcgtg gtcctgaagg
    57241 gttaatgcct tcggacgata ccctgaaaaa ctggaaagtc agtaaaatta tctggcggcg
    57301 ttaatttgcg tctgaacaaa ccgccgccgc gaaaaaacac gtaaaatagc gctcaacaat
    57361 gccacggatt gcgtggcatt cttattttca ggaggaacaa tgtctcaggt tcagagtggc
    57421 attttgccag aacattgccg cgcggcgatt tggatcgaag ccaacgtgaa aggggaagtt
    57481 gacgccctgc gtgcggccag taaaacattt gccgacaaac tggcaacttt tgaagcgaaa
    57541 ttcccggacg cgcatcttgg tgcggtggtt gcctttggta acaacacctg gcgcgctctg
    57601 agcggcggcg ttggggcaga agagctgaaa gattttccgg gctacggtaa aggccttgcg
    57661 ccgaccaccc agttcgatgt gttgatccac attctttctc tgcgtcacga cgtaaacttc
    57721 tctgtcgccc aggcggcgat ggaagccttt ggtgactgca ttgaagtgaa agaagagatc
    57781 cacggcttcc gttgggtgga agagcgtgac ctgagcggct ttgttgacgg tacagaaaac
    57841 ccggcgggtg aagagacgcg tcgcgaagtg gcggttatca aagacggcgt ggatgccggc
    57901 ggcagctatg tgtttgtgca gcgttgggag cacaacctga agcagctcaa ccggatgagc
    57961 gttcacgatc aggagatgat gatcgggcgc accaaagagg ccaacgaaga gattgacggc
    58021 gacgaacgtc cggaaacctc tcacctcacc cgcgttgatc tgaaagaaga tggcaaaggg
    58081 ctgaagattg ttcgccagag cctgccgtac ggcaccgcca gtggcactca cggtctgtac
    58141 ttctgcgcct actgcgcgcg tctgcataac attgagcagc aactgctgag catgtttggc
    58201 gataccgatg gtaagcgtga tgcgatgttg cgtttcacca aaccggtaac cggcggctat
    58261 tacttcgcgc cgtcgctgga caagttaatg gcgttgtaag cgtttctgat tcgatgatta
    58321 aaggccagcc agtaacaaaa ctgactggcc tttttattac ttttgcttca acgccgcata
    58381 cacctgatca actacccaac cataagttgc aatcggtaac tgaccgcttt cgaacatatt
    58441 gggatttttt tgtggatcgg caactggcga atggggcttg ttgcttaaca tcacaatcgc
    58501 catatgattg accggatcga taacggttac tgtcccggtc cagccagtgt gaccgtaggt
    58561 ttgcgggctt gccagcgtgc caaacgtcgg cgtcatggtg gcattaccat tcacgcgcca
    58621 gccgaggcca aaagtggcat cttccttaga gctggtggtg aacatcttca ccgtttccgc
    58681 actgaacagc tgcacatcgc catagccccc gccgttcagc atcgtttgca ttaacaccgc
    58741 aatatcgccg gtattggaaa acaaacctgc gtgcccggaa acgccgccca tcgaataaaa
    58801 ggctttttca tcgtgcacct gaccccagag agtggaggtg cggatattcg ggaaatggat
    58861 cacgccatcg cgggtattgc cgttcaattc cgtggcggca atttgttgcg gtttaaagcc
    58921 tttcagcagc gggttaaata ccgtatgtgt caggccgagc gggcgataaa tcgactcttc
    58981 aacatagcgg tcaagcggct gaccggtaac cgactcgacg ataaatccaa gcagcatata
    59041 atcgacatcg ctgtagatat gtttgctgcc gggctgatat tccagcggcg tgcgcttgat
    59101 catctccagc gtctggcctt tatcctggga atataacgcg cccgcgaccg ctttattcgg
    59161 gtattgcgga tctgccggga aaccgccgct gtgatgcagc aggtcagaaa tccgcagggt
    59221 gtttttgcct ttaatggtgt catttggact atcggcaaac cccggaatat atttcgcaat
    59281 ccgatcgtca ggatgcagtt tgccttcgct catcagcttt tgcagggcga agttcgtggc
    59341 gtacattttg gtgtttgagg ccaggtcata cagcgtcccg gtggtggctt tgacaggctg
    59401 ttccatcaac acgctgccat cgtacttttt cgccgctccc caggctttgc gatacacaat
    59461 ctgattatct ttaatgatca gcaggtttac gccgggataa ccgacatcaa tttgctggct
    59521 aatccagcga tccatctggt taagccgttc gacgttaaac cctgctttct ctggcgagct
    59581 ttctgtcaga acagggtatt ttgcggcgct gacgctacaa ctaacagcca gcagagaaag
    59641 atagagcatt gtccgtttca tgatatcagt ccagattgac gttacggcag ccaaagagcg
    59701 tggtgaaaat aaacccgcaa acccatgcca ccagaatccc gccagcataa accgccatgg
    59761 ccggaaggat gccttgtgcg gaagtcatca gcggcagcgc taccagacca gacggcccaa
    59821 aggcgctgtt taagcccatc ggcagacccc atcaggctat caggccgata aacaaaccgc
    59881 ccgccgcgcc gcctaaacag gcggtaacaa acggcttcat gcggggcagg gtgacaccgt
    59941 aaatcagcgg ttcaccaacg cccagcaggc cgggaataat cgccccgcgt acctgactgc
    60001 gtagcgcact gtgcggttgc gcccgccagt agagtgccag cgccgcgccc acctggcccg
    60061 cgcctgccat tgaaaggatg ggaaataagc tgttgaatcc ctagctgtcc attaacgcga
    60121 ggtaaacagg aataaagccc tgatgcacgc caaataccac ggcgatcagg aacagcccgg
    60181 ctaaaaccgc acaaccgaac ggattactgt tcaggtgcat aaacagccat gacatacctt
    60241 cgaataacca gccacccagc ggcataatga tcaggtacgc aagcgtagcg gtgatcagca
    60301 gggtgattaa cgaggtcagc agcatgtcga gatcgtccga cataaagcgg cgcaccatgc
    60361 cttcaatgcg ggcgcaggcc cacgcggcaa tcagcacgcc gataatattg ccgcgcggat
    60421 cgatgggcag accaaagaaa tcgtgaaaac cggcgtagtc accggtggtt gcagcggggt
    60481 tataaccgag caaaaagagt gcggcaataa ttgcgccatt gacgcccgtg ccgccaaatg
    60541 cctgggcggc gttatagccc accagaatca ccaggaaagt gaacaaacct ttgctgaaca
    60601 ccttcataaa attcagcgcg tcgggtagcg tgccctgagc atctgccgga acgtgcatca
    60661 ccgtggcaat taacgtcgct atccccagca gcagaccggc ggcaataaaa ccggggatca
    60721 gcggcgtgaa gatggtggcg aatttggcga gaaattgttg cacgccggag gtttgtttgg
    60781 cttttaactg acgtttgttc tgggcggcga tttctgcggc atcctgtacc ggtgcctcgc
    60841 ccagcagctc gctcatcgct tttgcggcac gatgcgcttt accaggccca aaaacgacct
    60901 gcacctggtc gctggtcaaa atgacgccct tcacgccttc cagcgttttg atattgggat
    60961 caaccagtga actgtcatgt acacccagac gcaggcgcgt catacagtta ccacaactgg
    61021 cgatatttcc cagtccgccg acacgggtaa gaatggtgtt cagaagttca ctgctgatct
    61081 ctttggccat gggttattcc ttgtctaaaa cctgacgaat aaagccgccg tgttgatcca
    61141 ggcgtttttt agcttctgcg gcatcgagat ttttcagcac catcacaatg gccgttttac
    61201 agttgcgctc gcaggcaatt aacgccgctt ccgcttgttc ggcattacat ccggtggcgt
    61261 ttttaacgat attgacctgg cgcacatgca gtttttcgtt ggtggcgacc acatcgacca
    61321 tcaggttgcc gaacactttg ccggatttaa tcatcagccc ggtggaaagc atattgagca
    61381 ccagtttctg cgctgtgcct gctttcattc gcgaagaacc agtgaccact tcggcaccta
    61441 caatcggtgt aatggcaaac tcggcggtgg tggaaacggc actccccgga ttacaggaaa
    61501 ttcccactgt gcggcaaccg agctggcggg cgtattccag tccggcaatc acatacggcg
    61561 tacgaccgct ggcggcaata ccgaccacca catcctgtgc cgttaaattt atatttttca
    61621 gatcgttaat gccgccttct cggctatctt ccgtgccttc caccgcgtgc tgaatggcat
    61681 attcgccgcc agcaatcaaa ccaaccacca gacccggttt cacgccgtag gtgggcggac
    61741 attcgctggc atccagaatc cccagacgac cggatgttcc cgcaccgaga taaatcagac
    61801 gaccgccgcc gcttacctgg gcgtggataa catcgatcgc cgcggcgata tccggcagga
    61861 cgcgctcaac ggcaagcggt acggttttat cttcatcgtt gataatccgg cacatttcca
    61921 gcgtcgatac gcggtcaatt tcagccgagg cggtgttcga gccttcagta atcatctttt
    61981 caagttgcat cggtggtgac cttactattt acgatcaaat aattgcagat gtatggaatc
    62041 atatatcccg aattgacaga cttaaaggaa tcattgagtc tacacttgca gagtgtgaag
    62101 agcgtcataa gaacttaagc ggaatttttg ctgccagccc gggagagtgg aagatgttgt
    62161 acctgacgaa aatacgcaat gcggaaagtg aatttaccga gaacgagcaa aaaattgctg
    62221 actttctgcg ggccaacgtg agtgagctga aatccgtctc ttcgcgcaag atggcgaaac
    62281 agctcggcat cagtcagtcg agcattgtga aatttgccca gaagctcggc gcgcaaggat
    62341 ttactgaatt gcgtatggcg ttaattggcg aatacagtgc cagccgggag aaaacgaatg
    62401 ccacggcgct gcacctgcac agttcgatta ccagtgatga ttcgctggaa gtgattgccc
    62461 gcaaactgaa tcgcgaaaaa gagctggcgc tggagcaaac ctgtgcgttg ttcgactacg
    62521 ctcggctgca aaaaatcatt gaggtcatca gtaaagcacc gtttatccag ataacgggcc
    62581 tgggcgggtc ggcgctggtg gggcgcgatc tgtcgttcaa actgatgaaa attggctatc
    62641 gcgtggccta tgaagccgac acacacgtac aagccaccgt ttcccaggcg ttgaaaaaag
    62701 gcgatgtaca aatcgctatc tcttacagcg gcagtaaaaa agagattgtc ttgtgcgcag
    62761 aagcggcacg aaagcagggg gcaacggtga ttgccattac ttctctggct gactctccgc
    62821 tgcggcggct ggcgcatttc acgctcgata ccgtttccgg tgaaaccgaa tggcgtagct
    62881 cgtcgatgtc tacccgcacg gcgcaaaatt ctgttaccga tttactgttt gttggtcttg
    62941 tgcaactcaa tgatgtggag tcattgaaaa tgattcagcg cagcagtgag ctaacacaac
    63001 gcctgaaatg atgcataaag cagcgactgg attgagattt tcctgaatta gtgagctgat
    63061 ccgcagcaat attttgttta tcctgtattt tcagagggaa tggagtgtaa cgctctgtat
    63121 taacaaggag agcattaaaa tgggtaaact cacgggcaag acagcactga ttacgggcgc
    63181 attgcaggga attggcgaag gaattgccag aacttttgcg cgtcatggcg caaacctaat
    63241 cttgctggat atctcccctg agatcgaaaa gctggcggac gaactgtgtg gtcgtggtca
    63301 tcgctgtacg gcggttgtcg ccgatgtgcg tgacccggcg tcggtagccg cagctatcaa
    63361 acgcgcgaag gaaaaagaag ggcgcattga tatcctggtg aataacgcag gcgtttgtcg
    63421 tctgggcagt ttcctcgata tgagcgatga cgatcgcgat ttccatattg acatcaatat
    63481 taaaggcgta tggaacgtca cgaaggcggt gctgccggag atgattgccc gcaaagatgg
    63541 tcgcattgtg atgatgtctt cagtcactgg tgatatggtg gccgatcctg gcgaaacggc
    63601 gtacgcctta acgaaagcgg cgattgttgg cctgacaaaa tcgctggcgg tggagtacgc
    63661 gcagtctggt attcgcgtta acgccatttg cccgggatac gtgcgcacac caatggcgga
    63721 aagcattgcc cgccagtcga acccggaaga tccagagtcg gtgctgactg aaatggcgaa
    63781 agcaatcccg ctgcgtcgcc tcgccgatcc gctggaagtc ggcgaactgg cggccttcct
    63841 cgcatcggat gaatccagct atttaaccgg tacacagaat gtgattgatg gcggcagcac
    63901 actgccggag acggttagcg tcggtatctg attcacctct gtttcctccc tgcatttgtg
    63961 gggaggattt cgttttaaac ccggttctta ttcccttttc aacttccaaa tcaccaaacg
    64021 gtatataaaa ccgttactcc tttcacgtcc gttataaata tgatggctat tagaaagtca
    64081 ttaaatttat aagggtgcgc aatggccgtt aacttactga aaaagaactc actcgcgctg
    64141 gtcgcttctc tgctgctggc gggccatgta caggcaacgg aactgctgaa cagttcttat
    64201 gacgtctccc gcgagctgtt tgccgccctg aacccgccgt ttgagcaaca atggaccaaa
    64261 gaaaacggcg gcgacaaact gacaataaaa caatctcatg ccgggtcatc aaaacaggcg
    64321 ctggcaattt tgcagggctt aaaagcagac gttgtcactt ataaccaggt gactgacgta
    64381 caaatcctgc atgacaaagg caagctgatc ccggcggact ggcaaacgcg cctgccaaat
    64441 aatagctcgc cattctactc caccatgggc ttcctggtgc gtaagggcaa cccgaagaat
    64501 atccacgact ggaacgacct ggtgcgctcc gatgtgaagc tgattttccc gaacccgaaa
    64561 acgtcgggta acgcgcgtta cacctatctg gcggcatggg gcgcagcgga taaagctgac
    64621 ggtggcgata aagccaaaac cgaacagttt atgacccagt tcctgaaaaa cgttgaagtg
    64681 ttcgatactg gcggtcgtgg cgcgaccacc acttttgccg agcgcggcct gggcgatgtg
    64741 ctgatcagct tcgagtcgga agtgaacaac atccgtaaac agtatgaagc gcagggtttt
    64801 gaagtggtga ttccgaaaac caacattctg gcggaattcc cggtggcgtg ggttgataaa
    64861 aacgtgcagg ccaacggtac ggaaaaagcc gccaaagcct accttaactg gctctacagc
    64921 ccgcaggcgc aaaccatcat caccgactat tactaccgcg tgaataaccc ggaagtcatg
    64981 gataaactga aagacaaatt cccgcagacc gagctgttcc gcgtggaaga caaatttggc
    65041 tcctggccgg aagtgatgaa aacccacttc accagcggcg gcgagttaga caagctgtta
    65101 gcggcggggc gtaattaatg tttgcagttt catcaagacg cgtgctgccg ggctttacct
    65161 taagcctcgg caccagtctg ctgtttgtgt gcctgatttt gctgctgccg ctctccgcgc
    65221 tggtgatgca gctggctcag atgagctggg cgcagtactg ggaggtgatc accaacccgc
    65281 aggtagtagc ggcctacaaa gtaacgctgc tgtcagcgtt tgtggcatcg atttttaacg
    65341 gcgtttttgg cctgctgatg gcgtggatct taacccgcta tcgctttccg ggccgcacgc
    65401 tgcttgatgc gctgatggat ttaccctttg cgctgccaac ggcagtcgcc ggtttaacgc
    65461 tagcctcgct cttttctgta aacggttttt acggtgaatg gctggcgaag tttgatatca
    65521 aagtcaccta tacctggctg gggattgcgg tggcgatggc ctttaccagc attccgtttg
    65581 tggtacgtac cgtgcagccg gtgctggaag agttaggccc ggaatatgaa gaagcggcag
    65641 aaacgcttgg cgcaacgcgc tggcagagtt tctgcaaagt ggtgctgccg gaactttctc
    65701 cggcgctggt ggcgggcgtg gcgctgtcgt ttacccgtag tcttggtgaa tttggcgcgg
    65761 tgatttttat cgccggaaat atcgcgtgga agacggaagt gacgtcgctg atgatttttg
    65821 tgcgcttaca ggagtttgat tacccggcag cgagcgcgat tgcttcggtg atcctcgcgg
    65881 catctctgct gctgctgttc tcaattaaca ctctgcaaag tcgctttggt cggcgtgtgg
    65941 taggtcatta atggcggaag ttacccaatt gaagcgttat gacgcgcgcc cgattaactg
    66001 gggcaaatgg tttctgattg gcatcgggat gctggtatcg gcgttcatcc tgctggtgcc
    66061 gatgatttat atcttcgtac aggcgttcag caaagggctg atgccggttt tacagaatct
    66121 ggccgatccg gacatgctgc acgccatctg gctgacggtg atgatcgcgc tgattgccgt
    66181 accggtaaac ctggtgttcg gcattctgct ggcctggctg gtgacgcgct ttaacttccc
    66241 tggacgccag ttactgctga cgctactgga cattccgttt gccgtatcgc cggtggttgc
    66301 cggtctggtg tatctgctgt tctacggctc taacggcccg ctgggcggct ggctggatga
    66361 acataatctg caaattatgt tctcctggcc cggaatggtg ctggtcacca tctttgtgac
    66421 gtgtccgttt gtggtgcgcg agctggtgcc ggtgatgtta agccagggca gccaggaaga
    66481 cgaagcggcg attttgcttg gcgcgtctgg ctggcagatg tttcgccgcg tcacattgcc
    66541 gaacattcgc tgggcgctgc tttatggcgt ggtgttgacc aacgcccgcg caattggcga
    66601 gtttggcgcg gtgtcggtgg tttccggctc gattcgcggc gaaaccctgt cgctgccgtt
    66661 acagattgaa ttgctggagc aggactacaa caccgtcggc tcctttaccg ctgcggcact
    66721 gttaacgctg atggcgatta tcaccctgtt tttaaaaagt atgttgcagt ggcgcctgga
    66781 gaatcaggaa aaacgcgcac agcaggagga acatcatgag cattgagatt gccaatatta
    66841 agaagtcgtt tggtcgcacc caggtgctga acgatatctc actggatatt ccttcaggtc
    66901 agatggtcgc gttgctgggg ccgtccggtt ccgggaaaac cacgctgctg cgcattatcg
    66961 ccgggctgga gcatcaaacc agcgggcata ttcgcttcca cggcaccgac gtcagccgcc
    67021 tgcacgcgcg cgatcgcaag gtcggtttcg tgttccagca ttacgcgctg ttccgccata
    67081 tgacggtatt cgacaatatc gcttttggcc tgacggtgct gccgcgtcgc gatcgctcga
    67141 atgccgcagc catcaaagcg aaagtgacaa aattgctgga gatggtgcag cttgctcatc
    67201 tggcggatcg ttatccggca cagctttccg gcggccagaa acagcgcgtg gcgctggcgc
    67261 gcgcgctggc tgtagaaccg caaattctgc tgcttgatga accgtttggc gcgctggatg
    67321 cgcaggtgcg taaagagctg cgtcgctggc tgcgtcaact gcatgaagag ctgaaattca
    67381 ccagcgtgtt cgtgacccac gaccaggaag aagcgacgga agtagctgat cgtgtagtgg
    67441 tgatgagcca gggcaatatt gaacaggctg atgcgccgga gcaggtatgg cgcgaaccag
    67501 cgacccgttt tgtgctcgaa tttatgggcg aagtgaaccg cctgcaggga accattcgcg
    67561 gcgggcagtt ccatgttggc gcacatcgct ggccgctggg ctacacacct gcttatcagg
    67621 ggccggtgga tctcttcctg cgcccgtggg aagtggatat cagccgccgt accagtctcg
    67681 attcgccgct gccggtacag gtactggaag ccagcccgaa aggtcactac acccaattag
    67741 tggtgcagcc gctggggtgg tacaacgaac cgctgacggt cgtgatgcat ggcgacgatg
    67801 ccccgcagcg tggcgagcgt ttattcgttg gtctgcaaca tgcgcggctg tataacggcg
    67861 acgagcgtat cgaaacccgc gatgaggaac ttgctctcgc acaaagcgcc tgataggttg
    67921 agtgaatgtt aaacgcccgg aggcgcttcc cgcgatccgg gctttttaat ggcaaggttt
    67981 gtaacctgta gacctgataa gacgcgcaag cgtcgcatca ggcaacacca cgtatggata
    68041 gagatcgtga gtacattaga acaaacaata ggcaatacgc ctctggtgaa gttgcagcga
    68101 atggggccgg ataacggcag tgaagtgtgg ttaaaactgg aaggcaataa cccggcaggt
    68161 tcggtgaaag atcgtgcggc actttcgatg atcgtcgagg cggaaaagcg cggggaaatt
    68221 aaaccgggtg atgtcttaat cgaagccacc agtggtaaca ccggcattgc gctggcaatg
    68281 attgccgcgc tgaaaggcta tcgcatgaaa ttgctgatgc ccgacaacat gagccaggaa
    68341 cgccgtgcgg cgatgcgtgc ttatggtgcg gaactgattc ttgtcaccaa agagcagggc
    68401 atggaaggtg cgcgcgatct ggcgctggag atggcgaatc gtggcgaagg aaagctgctc
    68461 gatcagttca ataatcccga taacccttac gcccattaca ccactaccgg gccggaaatc
    68521 tggcagcaaa ccggcgggcg cattacccat tttgtctcca gcatgggaac aaccggcact
    68581 atcaccggcg tttcacgctt tatgcgcgaa caatccaaac cagtaacgat tgttggcctg
    68641 cagccggaag aaggcagcag tattcccggc attcgccgct ggcctgcgga atatctgccg
    68701 gggattttca acgcttctct ggtggatgag gtgctggata ttcatcagcg cgatgcagaa
    68761 aacaccatgc gcgaactggc agtgcgggaa ggaatattct gtggcgtcag ctccggcggc
    68821 gcggttgccg gagcactgcg ggtggcaaaa gctaaccctg gtgcggtggt ggtagcgatc
    68881 atctgcgatc gtggcgatcg ctacctttcc accggggtgt ttggggaaga gcattttagt
    68941 cagggggcgg ggatttaaga ttattgcgca ggcaccgttt tatccgtctg cgcaattaag
    69001 gcatgtaaaa aaggctgtgc gttctcatcg ctcttaccgg ggatcttcac aatatacggc
    69061 ttcccggtaa tcgatgacga cgaagcaacc ttatcaataa actgctcggc agtgtcaatg
    69121 cggttacggg tattgccgag cttcagacgc agatgagaaa ccgcttcatc gcaggtatgt
    69181 tcatcaccgt tgcgcacaaa tatcaaatcc tttttctgtg ctaatccctc cagcatggcg
    69241 ttgatgcggg cttcttcatg aacagttaac ttcgcgtaag cggggagtgt cattagtagt
    69301 gtaatgacca gacaaatgat tttcttcatt gaattacctt atccttgttt tgtgatagcc
    69361 gatttgactg ccagaatcag gtaaagtttc ggttggaata cgatgaggat catcggcaag
    69421 gagaaaaaat taaatgagta gtttgttgtt gtttaacgat aagagtaggg cgctgcaggc
    69481 ggatatcgtc gccgtgcagt cgcaggtagt gtacggcagc gtaggcaaca gcattgcggt
    69541 gcctgctata aaacagaacg gcttgaatgt ctttgccgtg ccgacggtat tgctgagcaa
    69601 tacgccgcat tatgacactt tctacggtgg tgcgattccg gacgaatggt ttagcggcta
    69661 tttgcgtgcg cttcaggagc gtgatgcgct gcgccaactt cgtgctgtaa ccacgggcta
    69721 tatgggaacg gcatcgcaaa tcaaaatcct tgccgagtgg ctgactgcgc tacgcaaaga
    69781 ccatcctgac ctattgatca tggtcgatcc ggtgattggc gatattgata gcggaattta
    69841 tgtcaaacct gaccttcccg aggcgtatcg acaatattta ctgccgctgg cgcaaggaat
    69901 tacccccaat atctttgagc tggaaatcct gaccggtaaa aattgccgcg atctcgacag
    69961 tgccattgct gccgcaaaaa gtctgctttc agacacatta aaatgggtgg tgattaccag
    70021 cgcctccggt aatgaagaaa atcaggagat gcaggtggtg gtggtcagtg ccgacagcgt
    70081 gaatgtcatt tcccattcac gggtaaaaac cgacctgaaa gggactggcg acctgttttg
    70141 tgctcagctt atcagtggtt tgctgaaagg gaaggcgtta aacgatgcgg tgcaccgtgc
    70201 ggggttgcgc gtactggaag tgatgcgcta tacccagcag catgagagcg atgaattgat
    70261 tttgccgccg ctggcggaag cataaaaaat ggcgccgatg g
//
LOCUS       DAEEKQ010000006        64811 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.006, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000006 DAEEKQ010000000
VERSION     DAEEKQ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64811
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.006"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(35..1333)
                     /gene="eno"
                     /locus_tag="KLK57_000382"
     CDS             complement(35..1333)
                     /gene="eno"
                     /locus_tag="KLK57_000382"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HBE0890864.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAADKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="KLK57_000383"
     CDS             complement(1421..3058)
                     /gene="pyrG"
                     /locus_tag="KLK57_000383"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HBE0890865.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="KLK57_000384"
     CDS             complement(3286..4077)
                     /gene="mazG"
                     /locus_tag="KLK57_000384"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HBE0890866.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="KLK57_000385"
     CDS             complement(4148..4483)
                     /gene="mazF"
                     /locus_tag="KLK57_000385"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HBE0890867.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="KLK57_000386"
     CDS             complement(4483..4731)
                     /gene="mazE"
                     /locus_tag="KLK57_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HBE0890868.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(4809..7043)
                     /locus_tag="KLK57_000387"
     CDS             complement(4809..7043)
                     /locus_tag="KLK57_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HBE0890869.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="KLK57_000388"
     CDS             complement(7091..8392)
                     /gene="rlmD"
                     /locus_tag="KLK57_000388"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HBE0890870.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPGRETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPQLEALLPKVMACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8449..11205
                     /gene="barA"
                     /locus_tag="KLK57_000389"
     CDS             8449..11205
                     /gene="barA"
                     /locus_tag="KLK57_000389"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HBE0890871.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="KLK57_000390"
     CDS             complement(11437..12777)
                     /gene="gudD"
                     /locus_tag="KLK57_000390"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBE0890872.1"
                     /translation="MSSQFTTSVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPYGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12798..14138)
                     /locus_tag="KLK57_000391"
     CDS             complement(12798..14138)
                     /locus_tag="KLK57_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HBE0890873.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPGGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="KLK57_000392"
     CDS             complement(14140..15492)
                     /gene="gudP"
                     /locus_tag="KLK57_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HBE0890874.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSHIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(15927..16376)
                     /locus_tag="KLK57_000393"
     CDS             complement(15927..16376)
                     /locus_tag="KLK57_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HBE0890875.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16394..17176)
                     /gene="truC"
                     /locus_tag="KLK57_000394"
     CDS             complement(16394..17176)
                     /gene="truC"
                     /locus_tag="KLK57_000394"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HBE0890876.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17176..17505)
                     /locus_tag="KLK57_000395"
     CDS             complement(17176..17505)
                     /locus_tag="KLK57_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HBE0890877.1"
                     /translation="MTTHDRVRLQLQTLEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18127..18672)
                     /gene="syd"
                     /locus_tag="KLK57_000396"
     CDS             complement(18127..18672)
                     /gene="syd"
                     /locus_tag="KLK57_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HBE0890878.1"
                     /translation="MDDLTAQALKDFTARYYDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            18740..19588
                     /gene="queF"
                     /locus_tag="KLK57_000397"
     CDS             18740..19588
                     /gene="queF"
                     /locus_tag="KLK57_000397"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HBE0890879.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19700..21064
                     /gene="ppnN"
                     /locus_tag="KLK57_000398"
                     /gene_synonym="ygdH"
     CDS             19700..21064
                     /gene="ppnN"
                     /locus_tag="KLK57_000398"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HBE0890880.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLFLTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            21620..22909
                     /gene="sdaC"
                     /locus_tag="KLK57_000399"
     CDS             21620..22909
                     /gene="sdaC"
                     /locus_tag="KLK57_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HBE0890881.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            22967..24334
                     /gene="sdaB"
                     /locus_tag="KLK57_000400"
     CDS             22967..24334
                     /gene="sdaB"
                     /locus_tag="KLK57_000400"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HBE0890882.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            24446..25201
                     /gene="xni"
                     /locus_tag="KLK57_000401"
     CDS             24446..25201
                     /gene="xni"
                     /locus_tag="KLK57_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HBE0890883.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="KLK57_000402"
     CDS             complement(25306..26454)
                     /gene="fucO"
                     /locus_tag="KLK57_000402"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HBE0890884.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMHYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="KLK57_000403"
     CDS             complement(26482..27129)
                     /gene="fucA"
                     /locus_tag="KLK57_000403"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HBE0890885.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRPIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYVATLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            27676..28992
                     /gene="fucP"
                     /locus_tag="KLK57_000404"
     CDS             27676..28992
                     /gene="fucP"
                     /locus_tag="KLK57_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HBE0890886.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQRLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPTLCFAVIFIFARFRSQTATN"
     gene            29025..30800
                     /gene="fucI"
                     /locus_tag="KLK57_000405"
                     /pseudo
     CDS             29025..30800
                     /gene="fucI"
                     /locus_tag="KLK57_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
     gene            30909..32327
                     /gene="fucK"
                     /locus_tag="KLK57_000406"
     CDS             30909..32327
                     /gene="fucK"
                     /locus_tag="KLK57_000406"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HBE0890887.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYADSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            32329..32751
                     /gene="fucU"
                     /locus_tag="KLK57_000407"
     CDS             32329..32751
                     /gene="fucU"
                     /locus_tag="KLK57_000407"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HBE0890888.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            32809..33540
                     /gene="fucR"
                     /locus_tag="KLK57_000408"
     CDS             32809..33540
                     /gene="fucR"
                     /locus_tag="KLK57_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HBE0890889.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="KLK57_000409"
     CDS             complement(33584..34684)
                     /gene="rlmM"
                     /locus_tag="KLK57_000409"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HBE0890890.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVDMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(34677..35072)
                     /locus_tag="KLK57_000410"
     CDS             complement(34677..35072)
                     /locus_tag="KLK57_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HBE0890891.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="KLK57_000411"
     CDS             complement(35091..36008)
                     /gene="gcvA"
                     /locus_tag="KLK57_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HBE0890892.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(36359..36586)
                     /locus_tag="KLK57_000412"
     CDS             complement(36359..36586)
                     /locus_tag="KLK57_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HBE0890893.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            36778..37983
                     /gene="csdA"
                     /locus_tag="KLK57_000413"
     CDS             36778..37983
                     /gene="csdA"
                     /locus_tag="KLK57_000413"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HBE0890894.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEDALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            37983..38426
                     /gene="csdE"
                     /locus_tag="KLK57_000414"
     CDS             37983..38426
                     /gene="csdE"
                     /locus_tag="KLK57_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HBE0890895.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="KLK57_000415"
     CDS             complement(38477..39283)
                     /gene="tcdA"
                     /locus_tag="KLK57_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HBE0890896.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="KLK57_000416"
     CDS             complement(39360..40457)
                     /gene="mltA"
                     /locus_tag="KLK57_000416"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HBE0890897.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            40666..40742
                     /locus_tag="KLK57_000417"
     tRNA            40666..40742
                     /locus_tag="KLK57_000417"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40700..40702,aa:Met,seq:cat)
     gene            40776..40852
                     /locus_tag="KLK57_000418"
     tRNA            40776..40852
                     /locus_tag="KLK57_000418"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40810..40812,aa:Met,seq:cat)
     gene            40886..40962
                     /locus_tag="KLK57_000419"
     tRNA            40886..40962
                     /locus_tag="KLK57_000419"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:40920..40922,aa:Met,seq:cat)
     gene            complement(41037..42290)
                     /gene="amiC"
                     /locus_tag="KLK57_000420"
     CDS             complement(41037..42290)
                     /gene="amiC"
                     /locus_tag="KLK57_000420"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HBE0890898.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            42522..43853
                     /gene="argA"
                     /locus_tag="KLK57_000421"
     CDS             42522..43853
                     /gene="argA"
                     /locus_tag="KLK57_000421"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HBE0890899.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IRRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQLDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(43915..45741)
                     /gene="recD"
                     /locus_tag="KLK57_000422"
     CDS             complement(43915..45741)
                     /gene="recD"
                     /locus_tag="KLK57_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HBE0890900.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEESLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHSHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPIVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRG"
     gene            complement(45741..49283)
                     /gene="recB"
                     /locus_tag="KLK57_000423"
     CDS             complement(45741..49283)
                     /gene="recB"
                     /locus_tag="KLK57_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HBE0890901.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYEHLLE
                     EVDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIDKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(49276..52164)
                     /gene="ptrA"
                     /locus_tag="KLK57_000424"
     CDS             complement(49276..52164)
                     /gene="ptrA"
                     /locus_tag="KLK57_000424"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HBE0890902.1"
                     /translation="MPRSTWFKALLLLVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPAMPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQVIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(52340..55708)
                     /gene="recC"
                     /locus_tag="KLK57_000425"
     CDS             complement(52340..55708)
                     /gene="recC"
                     /locus_tag="KLK57_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HBE0890903.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFSIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHQLGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIRAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLSAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(55721..56044)
                     /locus_tag="KLK57_000426"
     CDS             complement(55721..56044)
                     /locus_tag="KLK57_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HBE0890904.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56029..56436)
                     /locus_tag="KLK57_000427"
     CDS             complement(56029..56436)
                     /locus_tag="KLK57_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HBE0890905.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSIESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(56433..56996)
                     /locus_tag="KLK57_000428"
     CDS             complement(56433..56996)
                     /locus_tag="KLK57_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBE0890906.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(56987..57457)
                     /locus_tag="KLK57_000429"
     CDS             complement(56987..57457)
                     /locus_tag="KLK57_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HBE0890907.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(57642..58436)
                     /gene="thyA"
                     /locus_tag="KLK57_000430"
     CDS             complement(57642..58436)
                     /gene="thyA"
                     /locus_tag="KLK57_000430"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HBE0890908.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(58443..59318)
                     /gene="lgt"
                     /locus_tag="KLK57_000431"
     CDS             complement(58443..59318)
                     /gene="lgt"
                     /locus_tag="KLK57_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HBE0890909.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(59469..61715)
                     /gene="ptsP"
                     /locus_tag="KLK57_000432"
     CDS             complement(59469..61715)
                     /gene="ptsP"
                     /locus_tag="KLK57_000432"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBE0890910.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(61728..62258)
                     /gene="rppH"
                     /locus_tag="KLK57_000433"
     CDS             complement(61728..62258)
                     /gene="rppH"
                     /locus_tag="KLK57_000433"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HBE0890911.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNTSAYRRKRG"
     gene            62943..63632
                     /gene="mutH"
                     /locus_tag="KLK57_000434"
     CDS             62943..63632
                     /gene="mutH"
                     /locus_tag="KLK57_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HBE0890912.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            63701..64414
                     /locus_tag="KLK57_000435"
     CDS             63701..64414
                     /locus_tag="KLK57_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HBE0890913.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            64552..64770
                     /gene="ygdR"
                     /locus_tag="KLK57_000436"
     CDS             64552..64770
                     /gene="ygdR"
                     /locus_tag="KLK57_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HBE0890914.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
BASE COUNT        15709 a        17422 c        15890 g        15790 t
ORIGIN      
        1 gaggctggca tttttaaatc agataaagtc agtcttatgc ttggcctttg atctctttac
       61 gaccgttgta cggtgctttt tcgcccagag cttcttcgat acgaatcagc tggttgtatt
      121 tagcaacacg gtcagaacgg ctcatagaac cagttttgat ctggcctgca gcagtaccaa
      181 cagccaggtc agcgatggta gcgtcttcag tttcgccaga acggtgagag ataactgcag
      241 tgtagccagc atctttcgcc atcttgattg cagccagagt ttcggtcaga gaaccgatct
      301 ggttgaattt gatcaggatg gagttagcga tacctttttc gataccttct ttcaggatct
      361 tggtgttggt tacgaacagg tcgtcaccaa ccagctggat tttgtcgccc agaactttgg
      421 tctggtatgc gaaaccgtcc cagtcagatt cgtccagacc gtcttcgata gatacgatcg
      481 gatactgttt ggtcagttct tccaggaagt gagtgaattc ttcagaggtg aaagctttgt
      541 tgccttcgcc agccagaacg tatttaccgt ctttgtagaa ttcagaagct gcgcagtcca
      601 tcgccagagt gatgtctttg cccagttcat aaccagcagc tttaacagct tcagcgataa
      661 cagccagagc ttcagcgttg gaacccaggt tcggcgcata gccaccttcg tcaccaactg
      721 cagtgttcat gcctttcgct ttcagaactt ttgccaggtg atggaaaact tcagaaccca
      781 tgcggatggc ttctttcaca gttttcgcgc caaccggctg aatcatgaat tcctggatat
      841 caacgttgtt gtcagcgtgc tcaccaccgt tgatgatgtt catcatcgga accggcatgg
      901 agtatttgcc cggagtgccg ttcagttcag cgatgtgctc gtacagcggc atccctttat
      961 cggcagcagc agctttggcg ttagccagag atacagccag gattgcgttc gcgccgaatt
     1021 tggatttgtt ttcggtgccg tccaggtcga tcatgatctt gtcaatgcca gcctgatctt
     1081 tagcgtcttt gccaatcagt gcctgagcga tcgggccgtt taccgcagca acagctttgg
     1141 ttacgccttt acccaggaaa cgggatttgt cgccatcgcg cagttccaga gcttcacggg
     1201 aaccagtaga agcacctgac ggagcagctg ccataccgac gaaaccaccc tccagatgta
     1261 cttcggcttc aacagtcggg ttaccacggg agtcgatgat ttcacgaccg atgattttta
     1321 cgattttgga cattaggttt tcctcaagtc actagttaaa ctgaaactcc agacaaacaa
     1381 cgcgtaccca aggtacgcgt tgccgttcta acttttttac ttacttcgcc tgacgtttct
     1441 ggaactcgct ggcagctttc acaaagcctg caaacagcgg gtgaccatca cgtggagtag
     1501 aagtaaactc cggatggaac tggcaagcca cgaaccacgg gtgattcgga acttcgatga
     1561 tctcgaccaa ctgatcatcc ccggaacggc ccgcaacgcg cagacctgca tcttcaatct
     1621 gtttcaacag catgttgttg acttcgtaac ggtgacgatg acgctcaaca attgtcggcg
     1681 cattgtacag ctggcgaacc aggctatcgt caaccaactg gcactgctgt gcgccgagac
     1741 gcatggtacc gccgagatcg ctcttctcgc tacgaacttc aacgttgccg ttttcatcgc
     1801 gccactcggt aatcagcgcc acaaccgggt acttacagtc tggcacaaat tccgtagagt
     1861 tggcgttctc catgttggca acatggcgag cgtaatcaat taacgccacc tgcataccca
     1921 ggcaaatgcc cagataagga atattgttct cacgcgcaaa acgcgcggtc gtaatcatgc
     1981 cttctacgcc acgatagccg aaaccgccag gtacgaggat tgcgtccaga cctttaagga
     2041 tttcaacgcc gcgcgtttca acatcttgtg aatcgatcag tttgatgttg acgctgacac
     2101 gattcttcag cccaccgtgt ttcagtgctt cgatcactga tttataagca tccggcagtt
     2161 caatgtactt gccgaccata ccgatggtga cttcacttac cggattcgct tcttcgaaga
     2221 taacctgttc ccattcggac agatttgctt ccgggcagtt taagctgaat cgtttacaaa
     2281 tataatcgtc cagcccctga gatttcaaca ggcccggaat tttatagatg gaatcgacgt
     2341 ctttcagaga aataaccgct ttttccggaa cattacagaa caatgcaatc ttcgcacgtt
     2401 cgttcgccgg aacagcgcga tctgaacgac aaatcaggat gtcaggctgg ataccgatgg
     2461 agagcagctc ttttacagag tgctgagtcg gtttggtttt gacttcacca gacgctgcca
     2521 tgtacggcac cagcgtcagg tgcataaaca gagtgtgctc acggccaatt tcaacagcca
     2581 tctggcgaat cgcttcgagg aacggcaagg attcgatatc acctactgta ccgccgattt
     2641 ctaccagtac tacgtcatga ccttcgccac cttccagcac gcgctctttg attgcgttag
     2701 tgatgtgcgg aataacctgc acggttgcgc cgaggtagtc accgcggcgt tctttacgca
     2761 gaacgtcaga gtagatacga cccgtggtga agttgttgcg gcggctcatt ttggtacgaa
     2821 tgaaacgctc gtagtgcccc aggtccaggt cggtttcagc gccgtcttca gtaacgaaca
     2881 cttccccgtg ttggattggg ctcatagtac ctggatcgac gttgatgtac ggatccagtt
     2941 tcatgatggt cacattgagg ccacgggctt caagaatggc tgcgagggag gctgcggcaa
     3001 tgcctttacc cagagaggat acgaccccgc cggtcacaaa aatatagttc gttgtcatgc
     3061 tgaacctgag aagttaggtt gaaaagacga tggaataacc aggacgggaa agcagtatac
     3121 ccgaacatga cctgtgccac aaactttcat tatccctcct cttcgccagc gcactattga
     3181 aatcaggagt gagaaaatag cccctttggg gtaaatgttt ttgacgcaaa tcaagcgctt
     3241 gtcatttaaa aaatgacaca aatggcgctt gaccgcgtaa ttcccttaga gatcaatttc
     3301 ctgccgtttt acctgttgcc agacttcttc cattgtttcg aggtcaacac ctgtcatttc
     3361 cagtccacgc gcggcaacaa tacgctccac ttcgcgaaaa cgacgctcga atttttcgtt
     3421 cgctttttgc aatgcgattt ctgctttcgt ccctaaatgg cgagccagat taaccgtggc
     3481 aaacagcagg tcccccattt cctcctccag tttagcctgg tcgacaacag cctgccgcgc
     3541 ctcgtacatc acctcgtcga tctcttcgta gactttatca accaccgggc caagcgtcgt
     3601 ccagtcgaag ccaacgttgg cgcaacgttt ctggattttt tgcgcccgca ttaaagccgg
     3661 taaactacga ggaatatcgt ccagcgccga atgctgcgct ttctgcgcgc gctcttcggt
     3721 tttgatttgc tcccaacggg caagcacttc actactgttt tcggcagaac tatcagcaaa
     3781 aacatgcgga tggcgacgct ctaatttatc gctaatagca gcgcaaatat cattaaagtc
     3841 aaagcgccct tcttcctgag ccatttgcgc gtaaaacacc acctggaata gcagatcgcc
     3901 cagttcaccg cgaagatcgt caaaatcttc acgggcgatg gcgtccagca cttcgtaggt
     3961 ttcttcaagg gtgtaaggcg caatggtggc aaatgtctgc tctttatccc acgggcagcc
     4021 gttttccgga tcgcgcaggc gctgcataat agtgagcaaa cggtcgattt gattcattga
     4081 attgtcctga aaattgcggg tctgtcaggt ggaaacctgt gaccagaata gaagtgagtt
     4141 agtaacacta cccaatcagt acgttaattt tggctttaat gagttgtaat tcctctgggg
     4201 caactgttcc tttcttcgtt gctcctcttg cccgccaggc gatacttttt acctgatcag
     4261 ctaacgctac gccatcacgt tcctgaccgg ataaaacaac ttcgaacgga tatccttttg
     4321 attgcgttgt acaaggaaca cacagacaca tacctgtttt gttgttgtac atgaaaggac
     4381 tcaggacaac agctggacga tgtccagctt gctcgctacc ttttgtcggg tcaaaatcaa
     4441 cccaaatcag atcgcccata tcgggtacgt atcggcttac cattaccaga cttccttatc
     4501 tttcggctct ccccagtcga tattctcgtg gaggttttcc ggcgtgatgt cgttgaccag
     4561 ttcagcaagc gtaaatacgg gctctttacg cactggctca ataattaatt tgccatccac
     4621 caggtcaatc ttcacttcat catcaatatt cagattgagc gcctgcatta acgtagccgg
     4681 gatccgcacc gccggtgaat ttccccaacg ctttacgcta ctgtggatca taaccctttc
     4741 ctcaaaccgc tatcatatgt agatacagta tatatcaatc tacattgtag atacgagcaa
     4801 atttctgcct aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     4861 ttacccagca cgcggcccag cacctgcagg ttgtaaatct caatggtcat gtcgatggtt
     4921 gccagttgct gttttgtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     4981 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5041 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5101 gcatgagagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgc
     5161 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5221 tggtgcatca ggttgccaac accttcgact accacgcgac cgttatcttt actgcggttt
     5281 tgcggcgtgt agcttttttg ctgaagttgc ttcagcgcgg cggcgtcctg ctcttcggca
     5341 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5401 ccaccaatcg ccgccagcaa ctcgtcgaca tcattaaagt tgtaacgcgg cagcagatgt
     5461 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5521 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aagcgtgaat tttcgaacgc
     5581 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5641 tgtttctggg tgataatttc aatctggtcg cccatctgca gctggtaggt gaacggcaca
     5701 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5761 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     5821 acatacaccc ggtcgtcaaa gacctggctg cgtacttcgt cgagcatttc gccggaatca
     5881 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     5941 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6001 tctgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt gccacctggc
     6061 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacataatcg
     6121 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcatagcaa
     6181 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcaaaggcg
     6241 aggttctttt tctgcatttt acgccagatg ctgtagatgt gtttcggacg accatacact
     6301 tccgctttaa cgccttcagc tttcatctca gcgcgcagat gaccaacgaa ctcttcgatg
     6361 tagtgttcgc ggtcgagacg ccgttcatgc agcagtttgg caattcgttt gtattcggtt
     6421 ggatggaggt aacggaagca gtaatcttcc agttcccatt tcagttgtcc gattccgaga
     6481 cggttagcca gcggtgcgta gatgttggta cactcttttg ccgccagtac acgttcatct
     6541 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6601 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6661 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6721 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6781 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     6841 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     6901 ccctgcgtct gttgcagaca atacgcccaa gtttcggcta agcactcaca cgacttctgg
     6961 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7021 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7081 caaatcgcaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7141 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7201 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7261 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7321 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7381 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgccg
     7441 gaacgccttc tacacccacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7501 ccataccgca gaacaaatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7561 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7621 aagttaagcg caacccattt gagtcatacc agggcatctc accagagacg gtttcgagta
     7681 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7741 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7801 ttgcctgtac cagttcaaca tgaccaagat ggcgcataac ttgtaagctg cccaagcatg
     7861 ccatgacttt gggcagcaat gcttcaagtt ggggcactaa aatggggcat tgtttaacgt
     7921 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     7981 agtagttcag acttaaacgc gcgcgacggc gatagcccca gggaacatcg gcgatcactt
     8041 cagagacttc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8101 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8161 tttcgcgtcc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8221 tatcttcagt aacagtaact tccgcgtttt cctgcggcaa taatccgggg ataaatagcg
     8281 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8341 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8401 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8461 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8521 ctgagtatct ttttcgttgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8581 ggagccagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8641 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8701 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8761 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     8821 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     8881 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gctggagctg
     8941 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttccag cgtgatgatg
     9001 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9061 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9121 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9181 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9241 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttagatct ggcgaaaaag
     9301 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9361 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9421 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9481 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9541 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9601 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9661 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9721 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9781 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
     9841 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccggtctg
     9901 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
     9961 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10021 attatcgaag ggccatccac ccagtgcctc gccggtaagc gcctggccta tgtcgaacca
    10081 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10141 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10201 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10261 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagtcaatgc agaaaaactc
    10321 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10381 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagc
    10441 aagctggcaa tgacagtcat ggcagttgat gacaaccccg ctaacctgaa acttatcggc
    10501 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcggtt
    10561 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gcctgacatg
    10621 gatggcattc gggcctgcga gctcatccac cagctccccc atcagcaaca aacgccggtt
    10681 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10741 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10801 aagcctggca gcggtatttc ctctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    10861 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    10921 ctagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    10981 gaggagcagc tggttggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11041 ggcagttgcg gctatagcgg tgtgccgcgt atgaagaatc tctgccaact gatcgaacaa
    11101 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11161 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11221 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11281 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11341 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11401 gccggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11461 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11521 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11581 acccagcccc ggtttttccg gcacctgaac cagaccgcct ttgatctcaa acggttcttt
    11641 ggtcaagcgc tgattgcctt cctgccaaat ccagtgcgta tcaatcgcgg tgattttacc
    11701 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11761 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    11821 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    11881 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    11941 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccat acggatcttc
    12001 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12061 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12121 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12181 atatttttca tacgccgctt ccgctaggcg caccaccgca tccggcgtca tcgcttcttc
    12241 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12301 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12361 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12421 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12481 gcgcccacca gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12541 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12601 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12661 attacgcgta aagaacggtg cgtgtgcccc actcagattc atcagcatac tgtcatgacc
    12721 cgccaccggg atgacctgca tttcagtaac aacagacgtc gtaaattgag aactcatatt
    12781 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    12841 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgccagg cagacgttta
    12901 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    12961 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13021 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13081 acatgggtaa acatcgccag agagatatcg aaatggttat tagaatggca gccccaggtc
    13141 agcccccagt cgtcgcaaag ctgcgccaca cggactgcac cggaaagcgt ccagaagtgc
    13201 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13261 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgccgaaa ctccgccata
    13321 acttcacgac cggagaaacc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13381 tcattcagcc ctttgcatag agaaatggct tcatcaagta gccaggcacc gttgggatca
    13441 acggtaatcc gcgcatcagg gaagcgtttc ttcaatgcac gaacagtgtc gatttcttgc
    13501 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13561 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13621 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcaccg
    13681 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13741 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13801 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccgaaggt atcaaaatcg
    13861 gctgcctgat tacctttatg cacctgctga accactttat tcaggcgcgc aacttcctgg
    13921 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    13981 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca caatattgcg ggtgaaatat
    14041 gcgttatgtg ccccaccaat attaagcaac atgctatcgt gcccggcgac cggaatgact
    14101 ttcatatcag taataacagg actggattgt gtcgtcatct tattgccccg cgacaggttt
    14161 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattaaggc
    14221 atgaacacca acataaatca gcgccccatt aaaggagcca gtcgtgccaa cgatataacc
    14281 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14341 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14401 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14461 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14521 aatgttcagc gatcccgtgc gacgcatcag ccagtcggaa ataatcccac ccagcacacc
    14581 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14641 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14701 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gttgtttgat
    14761 ctgcccccac ttcacgctga acggcacttt aactttggtg ttttgctgat ccatattgat
    14821 cagcgcgcca cccgcggcga tgtactccag ctctttctga tttacccccg gatgttgatt
    14881 tggctcgtgg atgactttca accagataaa gctgatgaca atccccagac cgcccataaa
    14941 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15001 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcgc
    15061 cggaaaccag gccgcaacaa tgtgactgtt gccggggaaa gatggcgctt cagcaagccc
    15121 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15181 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15241 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15301 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15361 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15421 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15481 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15541 acatagccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15601 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15661 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15721 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gttaaaacga
    15781 agaaataaag aagagtatga aatggatcgc ttgactccag gcaaacgcca gtaaaaatcc
    15841 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    15901 acgacattta ccgctcgctg aacatatcag gacaacagcg tgccccactg ttcgacccac
    15961 ggatttgatt ccgtttccgg ttccgggttc tcactggcgt caatcaacag catttcgcca
    16021 acccgctgag cgctctgttc ctgcaacaag gcatcgaact gtttgccgcc attgcagaaa
    16081 tttacataac tgctgtcgcc gagcgcaatc acgccataac gcagattcgg ctggaagccc
    16141 agactatctt tgattccctg aaagagcggc acaatgctat caggaaggtc gccctgcccg
    16201 gtcgtggacg taaccaccag aacatactta tcctgataag gcagccagtc gcttaattca
    16261 ggatcttcaa ataccgttgc tttgtggccc tgtgcggtca gaattgcttc tgcttcttcc
    16321 gccactaaca gcgaatttcc gtacatggtg ccgacaaaaa taccaatttc cgccataccg
    16381 ttttctccct ggattaggaa cttatctctc catcctgacc cgatggcgca ctgaactcaa
    16441 ccctttcatt ttcaggaagc agaccgcgcc agccaaattg tgataacgcc tgcatccagg
    16501 tgtcgtccag gcccgcgtga atagtcagcg gctcgccagt aaaaggatgc gtcaatgaca
    16561 actgactggc atgtagcatt aaccgctgga ggccaaaatg ctcagcaccg ctgcgattct
    16621 ggcgtaaatc gccatgtttg ctatcgccaa taatcggatg acgcaaatgg gcaagatgtc
    16681 ggcgaagctg atgtttgcgt ccggttttcg gctccagttc caccaggccg tagcgcgtgg
    16741 tcgggtaacg tccggtcgct accggcattt ctacggtcgc cagaccgcga taatgcgtca
    16801 ctgctggctg cgggccttta tcttcgcggg caaatttatc agcgattttg tccagttctt
    16861 ccaccagtgg ataatccagc accgcttctt ccatcaacca gccgcgcaca atcgcatggt
    16921 aacgtttctg gatttggtgc tgttcaaact gttgtgccag cagccgtccg gcctcgctgg
    16981 ataatcccat caacaacaca ccagaagtgg gtcggtccag acgatgagca gtaaaaacat
    17041 gctgtcctat ctggtcacgc acggtttgca tgaccactac tttctcgtcg cgatccagcc
    17101 agctacggtg aaccagccag ccggagggtt tatttaccgc aaccagccat tcatcctgat
    17161 agagtatttc cagcattagc tcgcatcatc cgcaaaaaga gcatccagtt tttccaactc
    17221 agccagaata agcgcgcgtt gcggatggtc cgtcgccagc gccatttcat aatagggtgc
    17281 aacagcaaaa gcgcccggta acggctggtt gttatttaac aaatcgtgca ttcgcgggat
    17341 cagcacccac tgcaaccact ccagtggttc catggtgtcc ataaagaacg gttgggtact
    17401 attaaattga tgcggctggg gttcatcgtt tcgccagtgc tgatgttcac gcagtaacgc
    17461 ttcaagcgtc tgcaactgga gacgaacgcg gtcatgagtg gtcataaagc aacttcaata
    17521 agaaaaactg ccgcgaagga tagcaggaat aaaaaaaggg agcactgtat tcacagtgct
    17581 cccggttcgt ttcgcagcat tccagctact tttgttgctc cctgctcatc cttgacaact
    17641 tttcctctgg ccttgcggcc aatcgttcat cctgaactat tgcttcctgc tcacaccacc
    17701 ccgatgtgat acttcatcct gaagtgtccc tggccttcct gacccaccga atcatcctga
    17761 ccggttctca ttctccatcc tggaggtgtc ctttaacgcg tcctgcgtca tcctcttcgc
    17821 ttcatccaga agcctttccc tgaaacacca tcctggtgtg tcctgcagaa gtgtcatcat
    17881 cctgatgttc acttcgttgt ctgactccct gtcgacgaag atagaatcgt ctttttcgcg
    17941 aagtcttaca aggcgcttac agacaatgcc ttaaggaaaa tttcatatga aagttaagaa
    18001 ttaacaatca acattttgat tttaaatgaa aataacgatt cttatctttt taagtattcg
    18061 cttatttcct ggcgatctcc tacagggtct gtaagagatc tctcacaacc agatggggta
    18121 tttggattac agaagaggct taagttgatt aaggaattcc gcaagatttg aggctaaatg
    18181 cgtccgttta cgtgttccca gcgtctcttt gcacacttca ccggagagat tacacaccga
    18241 gatgacctct aactcttctt ccagcgtcgc gataaatagc gtgggcggca gttttagcct
    18301 tttctgagtc accagatgac caatcagatt ttcctgcacc cgacggaagt cgtcttcact
    18361 ccaggtttgc agcaatgtca gtttgatatc gccaaactgc gcgtgcatat ccccggcaaa
    18421 ctgagtcgta taaaaggtat gaattgtggg ttgtatcaca atatcaaaag cgcgttcaac
    18481 cgcgtttaca ttttgttccc cggtaaacgg ctggggttgc cagtatacgg catcttcggt
    18541 agttgaaata atgcacggtg aaggaacacc gtacagttcc tcacttaacg gccagctttt
    18601 atgctcttca tgccacgcat cgtagtagcg tgcagtaaaa tctttcaggg cttgtgcggt
    18661 caaatcgtcc actaatttct ctcttcactt aaaccagata cacttgtcgt ttagtttatc
    18721 tggtttatga cggtgaaaca tgtcttctta tgcaaaccat caggcacttg cgggcctgac
    18781 tcttggaaaa tcaaccgatt accgggatac ctatgacgcc agcctactgc aaggcgttcc
    18841 acgcagcctg aatcgcgacc cgctgggtct gaaagcggat aacctgcctt ttcacggtac
    18901 ggatatctgg acgctgtatg aactttcctg gctgaatgcg aaaggtttgc cgcaggtcgc
    18961 tgtcggtcat gttgaacttg attacaccag cgtaaatctg attgagtcga agagttttaa
    19021 gctctatctc aacagtttta accagacgcg ttttaataac tgggatgagg tgcgccagac
    19081 gctggagcgc gacttaagca cttgcgctca gggtaagatt agcgtggcgt tatatcgtct
    19141 tgatgaactg gaaggccagc cgataggtca ttttaatggc acttgcattg atgaccagga
    19201 tatcactatc gataactatg aattcactac tgactatctg gagaatgcca cctgtggtga
    19261 aaaagtagtg gaagagacgc ttgtcagcca cctgctgaaa tcaaactgcc tgatcaccca
    19321 tcaaccagat tggggttcga tccaaattca gtatcgtgga cgccaaattg acagagaaaa
    19381 actgctgcgt tacctggtct cattccgtca tcacaacgag ttccacgaac agtgcgtgga
    19441 acgcatcttt aatgacctgt tacgcttctg ccagccagaa aaattgagcg tttacgcacg
    19501 ttatacccgt cgtggcggtc tggacattaa cccgtggcgc agtaatagcg attttgtccc
    19561 atcgaccaca agactggttc ggcaataaat tttttctcaa ttttgcgtgc tggattcacg
    19621 cagaaggttg tgaaaggaca tcaggcaggg ctattgtaat caaagggaat gacgatattc
    19681 gtcccataag gagtttttct tgattacaca tattagcccg cttggctcca tggatatgtt
    19741 gtcgcagctg gaagtggata tgcttaaacg caccgccagc agcgacctct atcaactgtt
    19801 tcgcaactgt tcacttgccg tactgaactc cggtagtttg accgataaca gcaaagaatt
    19861 gctgtctcgt tttgaaaatt tcgatattaa cgtcctgcgc cgtgaacgcg gcgtaaagct
    19921 ggaactgatt aatcccccgg aagaggcttt tgtcgatggg cgaattattc gcgcgttgca
    19981 ggccaacttg ttcgcggttc tgcgtgacat tctcttcgtt tacgggcaaa tccataacac
    20041 cgttcgtttt cccaacctga atctcgacaa ctccgtccac atcactaacc tggtcttttc
    20101 catcttgcgt aacgctcgcg cactgcatgt cggtgaagcg ccaaatatgg tggtctgctg
    20161 gggcggacac tcaattaacg aaaacgagta tttgtatgcc cgtcgcgtcg ggaatcagct
    20221 gggcctgcgt gagctgaata tctgcaccgg ctgtggtccg ggagcgatgg aagcgccgat
    20281 gaaaggtgct gcggtcggac acgcacagca gcgttacaaa gacagtcgtt ttattggtat
    20341 gacagagccg tcgattatcg ccgctgaacc gcctaacccg ctggtcaacg aattgatcat
    20401 catgccagat atcgaaaaac gtctggaagc gtttgtccgt atcgctcacg gcatcattat
    20461 cttccctggc ggtgtgggta cggcagaaga gttgctgtat ttgctgggaa ttttaatgaa
    20521 cccggccaac aaagatcagg ttttaccatt gttcctcacc ggcccgaaag agagcgccga
    20581 ctacttccgc gtactggacg agtttgtcgt gcatacgttg ggtgaaaacg cgcgccgcca
    20641 ttaccgcatc atcattgatg acgccgctga agtcgctcgt cagatgaaaa aatcgatgcc
    20701 gctggtgaaa gaaaatcgcc gcgacacagg cgatgcctac agctttaact ggtcaatgcg
    20761 cattgcgcca gatttgcaaa tgccgtttga gccgtctcac gagaatatgg ctaatctgaa
    20821 gctttacccg gatcaacctg ttgaagtgct ggctgccgat ctgcgccgtg cgttctccgg
    20881 tattgtggcg ggtaacgtaa aagaagtcgg tattcgcgcc attgaagagt ttggtcctta
    20941 caaaatcaac ggcgataaag agattatgcg tcgtatggac gacctgctac agggttttgt
    21001 tgcccagcat cgtatgaagt tgccaggctc agcctacatc ccttgctacg aaatctgcac
    21061 gtaatctccc tcccccggtg acgtttgttg ccgggttttc tcgtttttgg tcacataccc
    21121 atcaatttat ttcatttgtt atatgaatgt ttcttaccac cctcacgcga taaatatcat
    21181 tgcagttaat atgtcataca atttatgttg taacgcaaac gtttccctat tttcataaac
    21241 cacattttat cgctatgaaa aagaaattat cgccatgatt aactaaaagt attgattttt
    21301 tcagttcaac ctacatatat tgcgcgcccc ggaagaagtc agatgtcgtt taatgggcaa
    21361 atattgccct taaattctct tttacttttg atttacagag taaagcgttg ggataatcta
    21421 tcttccaagt agattattgt atttgggatc aagatcactg atagatacat aacttgtgtg
    21481 tatctttccg ccctcaaatt attacggcgg taaatgatta agccatcgcc gatagacaga
    21541 tttcattttt acggtcaggc accttcccga gctgaactgg ctaaaagctg aattatttgc
    21601 attcctccag gagaaataga tggaaacgac tcaaaccagc acgattgcgt cgaaagactc
    21661 tcgtagtgcc tggcgcaaga cagacaccat gtggatgctg ggcctttacg gcacggcaat
    21721 cggcgcgggc gtgctgttcc tgccaatcaa cgccggtgtt ggcggtatga tcccgctgat
    21781 catcatggct atccttgcgt tcccgatgac attttttgct caccgcggcc tgactcgctt
    21841 cgtactgtct ggtaaaaacc caggcgaaga catcaccgag gttgtagaag aacactttgg
    21901 tattggcgca ggtaaactga ttaccctgct ctacttcttc gctatctacc cgatcctgct
    21961 ggtttatagc gtggcaatca ccaatacagt tgaaagcttc atgtctcacc agctgggtat
    22021 gacaccaccg ccgcgtgcga ttctgtcgct gatcctgatc gtgggtatga tgaccatcgt
    22081 tcgcttcggt gagcagatga tcgttaaagc aatgagtatt ctggtattcc cgtttgttgg
    22141 cgtactgatg ctgctggctc tgtacctgat cccgcagtgg aacggcgcag cactggaaac
    22201 gctgtctctg gacactgcat ctgcaaccgg aaacggtctg tggatgaccc tgtggctggc
    22261 aattccggtt atggtgttct ctttcaacca ctctccgatc atctcttctt tcgccgttgc
    22321 gaagcgtgaa gagtacggcg atatggcaga acagaaatgc tcgaagatcc tggcattcgc
    22381 acacatcatg atggtgctga ccgtaatgtt cttcgtcttc agctgtgtac tgagcctgac
    22441 tccggcagac ctggctgcgg ctaaagagca gaacatctcg attctgtctt acctggctaa
    22501 ccactttaac gcaccggtta tcgcgtggat ggctccgatt atcgcgatta tcgctatcac
    22561 caaatccttc ctcggccact acctgggcgc acgtgaaggc ttcaacggta tggtgattaa
    22621 atctctgcgt ggtaaaggta agtctatcga aatcaacaag ctgaaccgta tcactgcgct
    22681 gttcatgctg gtaacgacct ggattgttgc caccctgaac ccgagcatcc tgggtatgat
    22741 tgaaaccctg ggcggcccaa tcatcgcgat gatcctgttc ctgatgccga tgtacgcaat
    22801 tcagaaagta ccggcaatgc gtaagtacag cggtcacatc agcaacgtat tcgttgtcgt
    22861 gatgggtctg attgcaatct ccgcaatctt ctactctctg ttcagctaag tcctttcgcg
    22921 ccgctttcgg gcggcgcttc ctccgtttta acacgatgta tttcctatga ttagcgtatt
    22981 cgatattttc aaaatcggca ttggcccttc cagttctcat accgttggac caatgaaagc
    23041 gggtaaacaa tttaccgacg atctgattgc ccgtaatctg cttaaagacg tgacccgcgt
    23101 ggtggttgac gtgtacggct cgctctctct gaccggtaaa ggccaccaca ctgatatcgc
    23161 cattattatg ggcctggcgg gtaacctgcc ggataccgtg gatatcgatt ccatccctgg
    23221 ttttattcag gatgtgaata ctcatggtcg cctgatgctg gcaaacggtc agcatgaagt
    23281 ggagttcccg gttgatcagt gcatgaactt ccatgcagac aacctttctc tgcatgaaaa
    23341 cggtatgcgc attaccgcgc tggcgggcga taaagtcgtt tacagccaga cttactactc
    23401 tattggcggt ggctttatcg ttgatgaaga gcattttggc cagcagaata gcgcaccggt
    23461 tgaagttcct tatccgtaca gttcagcagc cgatctgcaa aaacattgtc aggaaaccgg
    23521 gctgtcactc tctggcctga tgatgaaaaa tgagctggcg ctgcacagca aagaagagct
    23581 ggaacagcac ctggcgaacg tatgggaagt catgcgtggc ggtattgagc gcggtatttc
    23641 caccgaaggc gtgttgcctg gcaaactgcg cgttccacgc cgtgctgcgg cactacgccg
    23701 gatgctggtc agccaggata aaaccaccac tgacccgatg gcggttgttg actggatcaa
    23761 catgtttgca ctggcagtga acgaagagaa cgctgctggc ggacgcgtgg tgactgcgcc
    23821 gactaacggt gcgtgcggga ttatcccggc agtgctggcg tactacgaca agttcatccg
    23881 cgaagtgaac gctaactcac tggctcgtta cctgctggta gccagtgcca ttggttctct
    23941 ttataagatg aacgcgtcaa tttctggtgc tgaagtcggc tgccagggtg aagttggtgt
    24001 ggcgtgctca atggcggcgg ctggtctggc agaactgtta ggtgcaagcc cggcgcaggt
    24061 gtgcatcgcg gcggaaatcg ccatggagca caacctcggt ctgacgtgtg acccggtcgc
    24121 cggacaggtc caggtgccat gcatcgagcg taacgccatt gcggcagtaa aagcggtgaa
    24181 cgccgcacgt atggcgctac gccgtaccag cgagccgcgc gtctgcctcg ataaagttat
    24241 cgaaaccatg tacgaaacag gtaaagatat gaacgccaag taccgcgaaa cctctcgcgg
    24301 cggcctggca atgaagatcg ttgcctgcga ttaatcgctc tccagaggcc tcgttttgcg
    24361 aggcctcttc ccgatttctc atccagccgt agcctgttcc ggcatcgaat gttaccctta
    24421 tcgcctgatc tttaaggggg ttatcgtggc tgttcatttg cttattgtcg atgcactgaa
    24481 tcttattcgt cgcattcatg ccgttcaggg atcgccctgt gtcgaaacct gccagcatgc
    24541 gctcgatcag ctcattatgc acagccagcc aacccacgcg gtcgccgttt ttgatgatga
    24601 aaaccgcagt agcggctggc gtcatcagcg tttaccagat tacaaagcgg gtcgaccgcc
    24661 aatgccggaa gagttgcacg acgagatgcc tgcattacgc gccgcctttg agcaacgcgg
    24721 cgtcccgtgc tggtcaacca gcggcaacga agccgatgac ttagccgcca cgctggcggt
    24781 caaagtgaca caggccgggc atcaggcaac gattgtttcg acagataaag gctactgtca
    24841 gttactttca ccgacattac gtattcgtga ttacttccag aaacgttggc tggatgcgcc
    24901 atttatcgat aaagaatttg gcgttcaacc gcagcagttg cccgattact ggggacttgc
    24961 ggggatcagc agttcaaagg taccgggtgt tgcgggaatc ggaccaaaaa gcgccacgca
    25021 gctgctggtc gagtttcaga gtctggaagg gatatatgag aatctggatg cggttgccga
    25081 aaagtggcgc aaaaaattag aaacccataa agagatggcg tttctgtgcc gcgatattgc
    25141 ccgcttacaa accgatttgc atatcgacgg caatttacag caattgcggt tggtacggta
    25201 acggcgagcc ggatgcaaac gattatcaac acattgaatt taatgcactt atataccgga
    25261 taagacgcgg ctagcgtcgt atccggcatt atcacatcag cgcatttacc aggcggtatg
    25321 gtaaagctct acaatatcct caagcgttgc ttcacgcggg ttgccaccgg tacaaacatc
    25381 atccagtgcc gcctgcgcca gtgccggaat gtcttccttg cgtacaccaa catcacgcaa
    25441 atgtggcgga ataccgacat cacggttgag agcaaacacc gcttcaacag cggcattacg
    25501 cgcctcttcc aggctcatac cttccacttt cacgcccata acgcgcgcga tatcgcggta
    25561 cttctcaccg gtaaagtcag cgttatagtg catgacatgc ggtaacagga tggcgttcgc
    25621 aacaccgtgt ggagtgttgt aaaacgcgcc cagtggatgc gccataccat gcaccaaccc
    25681 taacccaaca ttcgagaagc ccatacccgc aacatactgc ccgagcgcca tttcttctcc
    25741 ggcatcctta tcaccagcaa ccgatcctcg cagcgcccca gcaatgattt caatcgcttt
    25801 aatgtgcagt gcatcggtta gcgcccacgc gccacgggta atatacccct caatagcatg
    25861 agtgagcgca tcgacacccg tcgcagcttt cagcgctgga ggcataccat ccatcatgtc
    25921 agcgtcaata aacgccacct gcgggatatc atgcggatca acgcaaacaa acttgcgccg
    25981 tttctcttcg tcagtgatca cgtagttaat ggtcacttct gccgcagtac ctgctgtggt
    26041 aggaattgcc agaatcggta cactgggttt attggtcggg gaaagccctt ccaggctacg
    26101 cacatcggca aactccggat tgttgctgat aatgccaatc gctttacaag tatcctgtgg
    26161 agaaccacca ccaatagcga tcaggtaatc cgcgccgcta ttctggaata caccgagccc
    26221 ttctttgacg acagtaattg ttgggttggg cactacgccg tcgtaaatcg cccatgccag
    26281 ccctgcagca tccatcttat cggtcacttt cgccaccacg ccgcattgca ccagcgtttt
    26341 atcggtgacg atcagcgcct tctgataacc acggcgtttc acctcatcgg ttaaagcccc
    26401 aacagcaccc cgaccaaacc atgccgtttc gttcagaatc attctgttag ccatcatcct
    26461 tctccttgtt gctttacgaa attactcttc aatccgtaac ccataggttt tgaatttctc
    26521 cagcactacg gcaatctctt catcgctcag cactggcacc gggtccgtaa tcgccagggt
    26581 cgccacgtaa agttgcgcca gcacttcaac ttcatgcgcc agccataacg ctttttccag
    26641 attcacctca caagcgataa gcccatgatg ttgtaacaaa gttgccttac gatttttgag
    26701 agccagcgca acatgttcag aaagttcgcg tgttccaaag gtcgcataag gcgcgcaagg
    26761 aatagaatta ccacctgccg ccgcaatcat gtagtgaata gcggggatcg gtcggttaag
    26821 aatggaaact gctgtgcaat gaacggcatg attgtgaaca accgcgttgg catccggtct
    26881 gctttgatag gccgccatat ggaaacgcca ttcgcttgag gggagctttc cttcctcatg
    26941 tttaccgttg ccatcaataa agacaatatg cgactccgtc agtttttcat atggaatgcc
    27001 tgtaggcgta atcagcatcc catcctgata acgtacactg acgttccccg ctgtcccctg
    27061 gttcagtccc aggcgggtca tttccaggca agtgtcaata atctgacgag caagtttatt
    27121 tcgttccatt agctacctct ctctgattca aaacagggca ataatgttgt tcctttcaca
    27181 ctattgaatt agccgtttaa ttacccacca tcttcttcct gattaacaag aaagaaattc
    27241 acaagcttat attttgtgac ctagctcaac taatcacagt aaataactgc aagttctctt
    27301 tttataaccc cattaaaaat gaccgctctt aaaaatattt atcaaaacgg tcatttttct
    27361 attcacccaa gccaggaatg cccgttttcg gcataaacaa ttaatacagt catctggttt
    27421 gtgtttttta tgatttattt tctgaaacgg gcatgaaatt tcgattatta aagtgatggt
    27481 agtcacataa agtcaccttc cagctaataa gtgtgaccgc cgtcatatta cagagcgttt
    27541 tttatttgaa aatgaatcca tgagttcatt tcagacaggc aaatattcac tgatatgaag
    27601 cccgaactcg ctggttttgc acttttgaaa acataaccga ttacgtgctt aagcttctga
    27661 acctaagagg atgctatggg aaacacatca atacaaacgc agagttaccg tgcggtagat
    27721 aaagatgcag ggcaaagcag aagttacatt attccattcg cgctgctgtg ctcactgttt
    27781 tttctttggg cggtagccaa taaccttaac gacattttat tacctcaatt ccagcaggct
    27841 tttacgctga caaatttcca ggctggcctg atccaatcgg ccttttactt tggttatttc
    27901 attatcccaa ttcctgctgg gatattgatg aaaaaactca gttataaagc aggaattatt
    27961 actgggttat ttttatatgc cctgggcgct gcattattct ggcccgctgc agaaataatg
    28021 aactacacct tatttttagt tggcctattt attattgcag ccggattagg ttgtctggaa
    28081 actgccgcaa acccttttgt tacggtatta gggccggaaa gcagtggtca cttccgctta
    28141 aatcttgcgc aaacatttaa ctcgtttggc gcaattatcg cggttgtctt tgggcaaaga
    28201 cttattttgt ctaacgtgcc acatcaatcg caagacgttc tcgataaaat gtctccagag
    28261 caattgagtg cgtataaaca cagcctggta ttatcggtac agacacctta tatgatcatc
    28321 gtggctatcg tgttactggt cgccctgctg atcatgctga cgaaattccc ggcattgcag
    28381 agtgataatc acagtgacgc caaacaagga tcgttctccg catcgctttc tcgcctagcg
    28441 cgtattcgcc actggcgctg ggcagtatta gcgcaattct gctacgttgg cgcacaaacg
    28501 gcctgctgga gctatttgat tcgctacgct gtagaagaaa ttccaggtat gactgcaggc
    28561 tttgccgcta actatttaac cggaaccatg gtgtgcttct ttattggtcg tttcaccggt
    28621 acctggctca tcagtcgctt cgcaccacac aaagtcctgg ccgcctacgc attaatcgct
    28681 atggcactgt gcctgatctc agccttcgct ggcggtcatg tgggcttaat agccctgact
    28741 ttatgcagcg cctttatgtc gattcagtac ccaacaatct tctcgctggg cattaagaat
    28801 ctcggccagg acaccaaata cggttcgtcc ttcatcgtta tgaccattat tggcggcggt
    28861 attgtcactc cggtcatggg ttttgtcagt gacgcggcgg gcaacatccc cactgctgaa
    28921 ctgatcccca cactctgctt cgcggtcatc tttatctttg cccgtttccg ctctcaaacg
    28981 gcaactaact gaacatattt tccgaataaa gtgaggaatc tgtaatgaaa aaaatcagct
    29041 taccgaaaat tggtatccgc ccggttattg acggtcgtcg catgggtgtt cgtgagtcgc
    29101 ttgaagaaca aacaatgaat atggcgaaag ctacggccgc actgctgacc gagaaactgc
    29161 gccatgcctg cggagctgcc gtcgagtgtg tcatttccga tacctgtatc gcgggtatgg
    29221 ctgaagccgc tgcttgcgaa gaaaaattca gcagctagaa tgtaggcctc accattacgg
    29281 taacgccttg ctggtgctat ggcagtgaaa ccatcgacat ggatccaacc cgcccgaaag
    29341 ccatttgggg ctttaacggc actgaacgtc ccggcgctgt ttacctggca gcggctctgg
    29401 cagctcacag ccagaaaggc atcccggcat tctccattta cggtcatgac gttcaggatg
    29461 ccgatgacac atcgattcct gccgatgttg aagaaaaact gctgcgcttt gcccgcgccg
    29521 gtttggccgt cgccagcatg aaaggtaaaa gctatctgtc gctgggcggc gtttcgatgg
    29581 gtatcgccgg ttctattgtt gatcacaact tctttgaatc ctggctggga atgaaagtcc
    29641 aggcggtgga tatgaccgaa ctgcgtcgcc gtatcgatca gaagatttac gacgaagccg
    29701 aactggaaat ggcactgacc tgggctgata aaaacttccg ctatggcgaa gatgaaaata
    29761 acaaacagta tcaacgcaat gccgagcaaa gccgcgcagt tctgcgcgaa agtttactga
    29821 tggcgatgtg tatccgcgac atgatgcaag gcaacagcaa actggccgat attggtcgcg
    29881 tggaagaatc acttggctac aacgccatcg ctgcgggctt ccaggggcaa cgtcactgga
    29941 ccgatcaata tcccaatggt gacaccgccg aagcgatcct caacagttca tttgactgga
    30001 atggcgtgcg cgaacccttt gtcgtggcga ccgaaaacga cagtcttaac ggcgtggcaa
    30061 tgctaatggg tcaccagctc accggcaccg ctcaggtatt tgccgatgtg cgtacctact
    30121 ggtcaccaga agcaattgag cgtgtaacgg ggcataaact cgatggactg gcagaacacg
    30181 gcatcatcca tttgatcaac tcaggttctg ctgcgctgga cggttcctgt aaacaacgcg
    30241 acagcgaagg taacccgacg atgaagccac actgggaaat ctctcagcaa gaggctgacg
    30301 cttgcctcgc cgctaccgaa tggtgcccgg cgatccacga atacttccgt ggcggcggtt
    30361 actcttcccg cttccttacc gaaggcggcg tcccgttcac catgactcgt gtcaacatca
    30421 tcaaaggcct gggatcggta ctgcaaatcg cggaaggctg gagcgtggaa ttgccgaagg
    30481 atgtgcatga catcctcaac aaacgcacca actcaacctg gccaaccacc tggtttgcac
    30541 cgcgcctcac cggtaaaggg ccgtttacgg atgtgtactc ggtaatggcg aactggggcg
    30601 ctaaccatgg ggttctgacc atcggccacg ttggcgcaga ctttatcact ctcgcctcca
    30661 tgctgcgtat cccggtatgt atgcacaacg ttgaagagac caaagtgtat cgtccttctg
    30721 cctgggctgc gcacggcatg gatattgaag gccaggatta ccgcgcttgc cagaactacg
    30781 gtccgttgta caagcgttaa tacattactc cctgatgtga tgcccggtcg ctccggctac
    30841 cgggcctgaa caagcaagag tggttagccg gataagcaat gttatccggc tatattgcag
    30901 gagcgattat gaaacaagaa gttatcctgg tactcgactg tggcgcgacc aatgtcaggg
    30961 ccatcgcggt taatcggcag ggaaaaattg ttgcccgcgc ctcaacgcct aatgccagcg
    31021 atatcgcgat ggaaaacaac acctggcacc agtggtcttt agacgccatt ttgcaacgct
    31081 ttgctgattg ctgtcggcaa atcaatagtg aactgactga atgccacatc cgcggtatcg
    31141 ccgtcaccac ctttggtgtg gatggcgctc tggtagataa gcaaggcaat ctgctctatc
    31201 cgatcattag ctggaaatgt ccgcgaacag cagcggttat ggacaatatt gaacagttga
    31261 tctccgcaca gcggttgcag gctatttctg gcgtcggagc ctttagtttc aatacgttat
    31321 ataagttggt gtggttgaaa gaaaatcatc cacaactgct ggaacgcgcg cacgcctggc
    31381 tctttatttc gtcgctgatt aaccaccgtt taaccggcga attcactact gatatcacga
    31441 tggccggaac cagccagatg ctggatatcc agcaacgcga tttcagtccg caaattttac
    31501 aagccaccgg tattccacgc cgactcttcc ctcgtctggt ggaagcgggt gaacagattg
    31561 gtacgctaca gaacagcgcc gcagcaatgc tcggcttacc cgttggcata ccggtgattt
    31621 ccgcaggtca cgatacccag ttcgcccttt ttggcgctgg tgccgaccaa aatgaacccg
    31681 tgctctcttc cggtacatgg gaaattttaa tggtccgtag cgcccaggtt gatacttcgc
    31741 tgttaagtca gtacgccgat tctacctgcg aactggatag ccaggcaggg ttgtataacc
    31801 caggtatgca atggctggca tccggcgtgc tggaatgggt gagaaaactg ttctggacgg
    31861 ctgaaacacc ctggcaaatg ttgattgaag aagctcgtct gatcgcacct ggcgcggatg
    31921 gtgtgaaaat gcagtgtgat ttattgtcgt gtcagaacgc tggctggcaa ggagtgacgc
    31981 ttaataccac gcgggggcat ttctatcgcg cggcgctgga agggttaact gcgcaattac
    32041 agcgcaatct acagatgctg gaaaaaatcg ggcactttaa agcctctgaa ttattgttag
    32101 tcggtggagg aagtcgcaac acattgtgga atcagattaa agccaatatg cttgatattc
    32161 cggtaaaagt tctcgacgat gccgaaacga ccgtcgcagg agctgcgctg ttcggttggt
    32221 atggcgtagg ggaatttaac agcccggaag aagcccgtgc acagattcat tatcagtacc
    32281 gttatttcta cccgcaaact gaacctgaat ttatagagga agtgtgaaat gctgaaaaca
    32341 atttcgccgt taatttctcc cgaactattg aaagtgctgg cagagatggg acatggagat
    32401 gaaattattt tttccgatgc tcactttccc gcccattcga tgggaccgca ggtgatccgc
    32461 gctgatggcc tgttggtgag cgacttgctc caagcgatta tcccattatt tgagctggac
    32521 agttatgccc cgccgctggt gatgatggca gcggtagaag gtgacactct cgatcctgaa
    32581 gtagaacgac gttaccgtaa tgcgctttca ctacaagccc cgtgtcctga catcatccgc
    32641 atcaatcgtt ttgcgtttta tgaacgggcg caaaaagcct ttgcgatcgt tatcacaggc
    32701 gaacgagcga agtacgggaa tattctttta aaaaaagggg taacaccgta atctcatatc
    32761 ggtacgcccg catgacgcgg gcggttatcg aatgatgggg tgaaaaatat gaaagcggca
    32821 cgccagcaag cgatagtcga cctgctgctg aaccatacca gcctgaccac ggaagctctc
    32881 tctgaacagc taaaggtcag taaagaaacc attcgtcgcg atctcaatga attacagacg
    32941 cagggtaaaa ttctgcgcaa tcatggacgc gctaaatata tccaccgtca aaatcaagac
    33001 agtggcgatc cctttcacat caggctgaaa agccattatg cgcataaagc agatatcgcg
    33061 cgcgaggcgc tcgcgtggat tgaagaaggg atggtgatag ccttagacgc cagttcaact
    33121 tgctggtatc tggcacgcca gttgcctgac atcaacattc aggtcttcac caatagccat
    33181 ccgatttgcc atgaactcgg taaacgcgaa cgcattcaac tgatcagttc cggcggcaca
    33241 cttgagcgca aatatggctg ttacgtcaat ccctcgctga tttcccaact taaatcgctg
    33301 gaaatcgatc tgtttatttt ttcttgtgaa gggatcgata gcagcggcgc actgtgggac
    33361 tccaatgcga tcaacgctga ttacaaatcg atgctattaa aacgtgccgc gcaatcgttg
    33421 ttattgattg ataaaagtaa atttaatcgt tcaggggaag cccgcatcgg gcatctggat
    33481 gaggtaacgc acattatttc tgatgagcgc caggttgcaa cttctttggt aacagcctga
    33541 cggaaagggt agcaggccga agacgacgcc ccggccttgc ctgttatcgc tcgtcgcgac
    33601 gaccacccac cgcagcccag atgcggcgga cgtgcaccgt cacttcttcg cgatcgtgat
    33661 acaactgccg cgcctgaatc tgagcattta tgccatgttc atcaagctgt gcctgaatat
    33721 acgccagatt gtgtgatact tcttcgtagc gttttttcat cggcagtttg agattgaaaa
    33781 tggtttcacg gcaccagcca ttaaccagcc actgcgccat caatgccgca actttagccg
    33841 gtttttcaac catatcgcat accatccagg agatattgct gcgtgtcgga cggaatttga
    33901 aaccgtcttc ccgcagccac gtcacctgcc cggtatccat cagactttgc gccatcgggc
    33961 cgttgtcgac ggaataaacc cacatgttgc gcttcaccag ttggtaggtc cagccgccag
    34021 ggcaagcgcc taaatccacc gcccacatcc cgttcgccag gcgttcatcc cactcatccg
    34081 caggaataaa cacatgaaat gcctcttcca gtttgagcgt ggaacgactc ggcgcctctg
    34141 ccggaaattt caggcgcgga atgcccatat agaacggcga attattgttg ctgtatgagt
    34201 aaccggtata gcagcagcct ggtgcaataa agaatacatg cacaaccgga cgcttcggcg
    34261 tttcatagtt cgccagcacc ccggcatcgc gcaaggcagc gcgtagcggc acggtgaatt
    34321 tacggcagaa tttcagcaac tctttgcttt cgttggtatc ggcaacttca acacgcagtt
    34381 caccgccctt ctctactacg ccctgcaaca tgtcgacaat gggggtaata cgatcttctg
    34441 gcggcaaatg ctgcaagagt tcccccacca caaaccactg gcgggcaaaa attaatgaac
    34501 tgaacggcag ctcacggatt aacttatcgc catcatcagg ttgataacat tcataaatga
    34561 cataacccgc attctctttc acgcgggcaa aaccgaaaat ttcccgctgg ccggctttat
    34621 cggtaatttc tgcggcgcac tctttttcaa agcccggacg gcacagcaat acaaccttat
    34681 tcatgactta cgcccttgcg ctttaaacgg atagcaccaa ctaacattaa cgcccagccc
    34741 gcgaggaagc tcacgccgcc aaccggtgtg acaaatgccc acaaacgcag atgggacagc
    34801 gccaggcaat aaaggctgcc gctgaacaac accgtgccta acgcgaggaa aacgctactc
    34861 cagtaaaacc agatactgat gcgacgctgc attgccaccg ccagacctaa gatcgccagc
    34921 gtatgaaacg cctggtattc gaggccggtc tggatccagc ccatctcaac ggcccccatg
    34981 gttttactta acacatgcgc gccaaaagcg cccagagcca caaaaatgaa gccgctaatg
    35041 gcggcgaaaa tcagcataaa acggctggtc atggtcgtac cctacgtaaa ttattgttca
    35101 taacgaaagc ggaatttttc ttgttcagca gcggctttcg ccaggatcca ttggcgaaag
    35161 gcggctattt tacccagttc tgcctggctg tcatgacaaa ccagataaaa agcgttttta
    35221 ctgaccagaa catcattaaa cgggcaaaca agacgtccgg cctcgatttc agattgcgcc
    35281 atcacgttat ttgccagcgc cactccctgc ccgtgaatag ccgcttgcag caccatggcg
    35341 ctatgactaa aaattggccc ttgctgaacg ttgatatgat ttaaccccaa ctgtcgggta
    35401 tatgtctgcc agtcacggcg tgacgcatca tgtaataacg tatgtttagc cagatcttcc
    35461 ggggtcttca agggtttttc gcctgtcagc agtagcggcg aacacaccgg caataaatat
    35521 tcggcgtaca gtttttccac ccgtagcccc ggccagttgc cccgaccata aaatatcgcc
    35581 acatcaacat catccgccag cttatcttcc tgacgatcaa ccgcctggat tcgaacgtca
    35641 attcccggat aagctgaatt aaagctggaa agtcgcggaa ccaaccaatg aatggcgaaa
    35701 ctggggagta aactgaccgt caacgccccc ttggcgctac gggcctggag tttacgcgtc
    35761 gcttcggtta attgcgaaaa tatctctttg atatcgagga aatagctttg cccttcctcg
    35821 gtcagcagga gtgaacgatt acggcggcgg aacagtttta gccccaaaaa atcctcaaga
    35881 gacttgattt gatgacttac tgcggcctgg gtcacgaaaa gctcttctgc tgcgcgagtg
    35941 aaacttaaat ggcgtgctgc ggcatcaaaa actcgtaagg catttagcgg tggtaatcgt
    36001 ttagacatgg cttttaaact ttgatgttaa attaatttaa caattagatc acactgtgta
    36061 acctattagt ttttttaatc tgagccatta taaattgtcc gttgagcttc taccagcaaa
    36121 tacctatagt ggcggcactt cctgagccgg aacgaaaagt tttatcggaa tgcgtgttct
    36181 ggtgaacttt tggcttacgg ttgtgatgtt gtgttgttgt gtttgcaatt ggtctgcgat
    36241 tcagaccatg gtagcaaagc tacctttttt cacttcctgt acatttaccc tgtctgtcca
    36301 tagtgattaa tgtagcaccg cctaattgcg gtgctttttt ttaccttgcg atcgcgaatt
    36361 actgatccag ttcgaccatc tctttcacgt cagtacggtt gatctgctgt ttgttgccat
    36421 tagcgtcttt atacgaaatc ataccggtat cgttatcagt ctgtggtttg ccgtcagaga
    36481 cgatggtacg tccgtcattg gtgtgcatca catagttcga accggaacag gcgctcaggg
    36541 caaaagtcag catacaggca gaaataattg cggcagtctt tttcatcatc ttctccttaa
    36601 agcgattaat aaatcttaat agtattgatt aacaggctaa aattaacgcc taacactatt
    36661 cagcatatgt tacttacgcg gcttcgccag gatatccaga taattctgat ggttagcact
    36721 ctccttgtat caaagtgaat tttgcgtcac gatcggtgca tcaagccgag gagtaccatg
    36781 aacgttttta atcccgcgca gtttcgcgcc cagtttcccg cactacagga tgcgggcgtc
    36841 tatctcgata gcgccgcgac cgcgcttaaa cctgaagccg tggttgaagc cactcgacag
    36901 ttttacagcc tgagcgccgg aaacgtccat cgcagccagt ttgccgaagc ccaacgcctg
    36961 accgcgcgtt atgaagctgc acgagagaaa gtggcgcaat tactgaatgc accggatgat
    37021 aaaactatcg tctggacgcg cggcaccact gaatccatca acatggtggc acaatgctat
    37081 gcgcgtccgc gtctgcaacc gggcgatgag attattgtca gcgtggcaga acaccacgct
    37141 aacctcgtcc cctggctgat ggtcgcccaa caaactggag ccaaagtggt gaaattgccg
    37201 cttaatgcgc agcgactgcc ggatgtcgat ttgttgccag aactgattac tccccgtagt
    37261 cggattctgg cgttgggtca gatgtcgaac gttactggcg gttgcccgga tctggcgcga
    37321 gcgattacct ttgctcattc agccgggatg gtggtgatgg ttgatggtgc tcagggggcg
    37381 gtgcatttcc ccgcagatgt tcagcaacta gatatcgatt tctatgcttt ctctggtcac
    37441 aaactgtatg gcccgacggg tatcggcgtg ctgtatggca aatcagaact gctggaagcg
    37501 atgtcgccct ggctgggtgg cggcaaaatg attcacgaag tgagttttga cggcttcacg
    37561 actcaatctg cgccgtggaa actggaagca ggaacgccaa atgtggctgg tgtcatagga
    37621 ttaagcgcgg cgctggaatg gctggcagat tacgatatca accaggcaga aagttggagt
    37681 cgcagcctgg cgacgctggc ggaagatgcg ctggcaaaac gtcctggctt ccgctcattc
    37741 cgctgccagg attccagcct gctggccttt gattttgctg gcgttcatca cagcgatatg
    37801 gtgacgctgc tggcggagta cggtattgcc ctgcgggccg ggcaacattg cgctcagccg
    37861 ctactggcag aattaggcgt aaccggcaca ctgcgcgcct cttttgcgcc atataataca
    37921 aagagtgatg tggatgcgct ggtgaatgcc gttgaccgcg cgctggaatt attggtggat
    37981 taatgacgaa cccgcaattc gccggacatc cgttcggcac aaccgtaacc gcagaaacgt
    38041 tacgcaatac cttcgcaccg ttgtcgcaat gggaagataa atatcgccag ttgatcatgc
    38101 tggggaaaca gcttccggct ttgccagacg agttaaaagc gcaggctaaa gagattgccg
    38161 gatgcgaaaa ccgcgtctgg ctgggatata ccgtgactga aaacggtaaa atgcacttct
    38221 ttggcgatag cgaagggcgc attgtgcgcg gcctgctggc ggtgctgttg actgccgttg
    38281 aggggaaaac cgccgccgaa ttgcaggcac agtcgccgct ggcattgttt gatgagctgg
    38341 gattgcgtgc gcaacttagc gcctcacgca gccaggggtt aaatgcgtta agcgaggcga
    38401 ttatcgctgc gacgaagcag gtttaagcat agaaccgggt cggataagac gttcgcgtct
    38461 catccgacct gattgtttaa ccctgacgcg ccgctttcgc catcatcttc ttcagcgcat
    38521 gagaaaccgc aacaaaccca aaggtggcgg tcaccatcgt tgccgcgcca aatccagatg
    38581 cacaatccat ccgcttcggc ccttctgccg tggctttcat cgcacacaca gtaccgtctg
    38641 actgcgggta caccagcgct tcagtagaaa acacgcaatc gacgccgagc ttacctttac
    38701 tgtttttcac tacgccaaaa tcgcttttca ggcgctcgcg caacttcgcc gccagcggat
    38761 cctgaatcgt tttcgccaga tcggtaacct gaatctgcgt cgggtcaatc tgcccacccg
    38821 cgccaccggt cgtgaccagc gggattttat tgcgccgaca ataagcaatc agcgccgctt
    38881 tgggccgtac actatcaatg gcatcaatca cgtacgaata acccacgctc atatactgcg
    38941 ctacgttatc cggcgtcacg aaatcatcca ccaccgttac acggcactcc gggttaatct
    39001 ggcgaatacg ctccgccatg acttccgctt ttgccagccc gacgttatcg cgcagagcgt
    39061 gaatttgccg attggtattg gtgacgcaca catcatccat atcgataagc gtgattgcgc
    39121 caatccccgt gcgcgccagc gcttccgccg cccaggaacc gacaccaccg ataccaacca
    39181 cacaaatatg cgcgtcagca aacagttgca acgctttttc accatacaga cgcgctgtgc
    39241 caccaaaacg ctgacgccat gcatcactaa ttaccacaga catagcacct cagaattaaa
    39301 aagggtgagg ttatccctca ccctgtcgta tctgtaaaaa cggtatacag aataacacat
    39361 cagccgctaa agacgttacc tgcgcccggg gcggttttca gcacccagac acgtccatag
    39421 tggttgtacc aacctgcgcg gtgtccggct tccggcccga tcccttgata gatatcgaag
    39481 tgttggcctt tgattgcacc accgacatcc agcgccacca tcagacgcag ttcgtactga
    39541 ccattaaatt tgccgttatt atccagcaac ggcacttctg ccagcaaggt agtacctggc
    39601 ggaataatgg aacgatcaga ggcaactgac gcgcgaccaa ccagcggcac cgcacttgcc
    39661 cctttcaccg gtgcaaaaga ttgcggttta aagaagacga aagacgggtt ctgttccagc
    39721 agctcgcgaa cctcggcttc actgtgtgtt tcgccccagt gacgaatcgc ctgcatcgac
    39781 atatcttctt ttttcacttc gccacggtcg atcagcacct taccaatgct gcgataggca
    39841 tgaccgtttt tccctgcata gctgaaaaag ttaagcggac tgccatcacc aaagtcgata
    39901 tacccactac cctgcacatc cataatgaag ttatccatca gggagttact gtaagcgaga
    39961 atatatttat cactcaatgc ccccgcgtag atctccgcac gagacggcag acgaccacgt
    40021 tttggcggca tacggtaaat aggatactgg aactcgccct ggcgggtatg gcgcgcctga
    40081 attaccggcg tgtaatagcc tgtaaactgc acgttaccat agttgtcggc accttccatc
    40141 tgccaggcat caatgccgaa ctggcgcata ttgcgggtat caccgcctgc gcgcagccac
    40201 tcttgcaccg cgttataaac attactctgg ttgccataca gacgcggtga cgaattacgg
    40261 atatggttaa tttgctcggc aaaatcaccg gcgttaatcg gcgcgccaac ggcatctggc
    40321 tggttcacca gagagaaagg ctgggtaaat ttcccgtctt tatattgctg tccgcgatcg
    40381 gttggtttgg aagagcaggc ggcaagcatt gccacaaccg tgcccataag aaggtacttt
    40441 acccaacgtc ctttcattgt tctcttcttc aggttaaaaa ataaggcgca acgaagataa
    40501 caaaccgccg cgtttaatga aatgagcagc ctctccctga cgcaaatttt gcacaaaaaa
    40561 taggctttag tgatttgttt ttgttcaaaa tcatgccaga tccgtgatcg gggtaaaaaa
    40621 aaggttgcat gaaaacgcga gcggagtata gtgcgcatcc acggaggcgg ggtggagcag
    40681 cctggtagct cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac
    40741 caattaaaat ttgatgaagt aaagcagtac ggtgacgcgg ggtggagcag cctggtagct
    40801 cgtcgggctc ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac caatcaaatt
    40861 tgatgaagta aaagcaatac ggtgacgcgg ggtggagcag cctggtagct cgtcgggctc
    40921 ataacccgaa ggtcgtcggt tcaaatccgg cccccgcaac caattattga acaccctaac
    40981 gggtgttttt ttgtttctac tctcccataa aaaaagcgcc attcagcgcc tttttatcat
    41041 ccccttctcg ccagcgttgc cccatcggca aaatacgctt taatccccgc aagaatagac
    41101 tccgcaactt cctgctggaa agtcgccgtt ttcagtttac gctcttcctc aacgttactg
    41161 ataaacgccg tttcgaccag aatggaggga atatctggtg cctttagtac ggcaaacccg
    41221 gcctgttcaa cttgattttt atgcagcttg ttgattttac cgagcttatt cagcactgct
    41281 ttaccaaact taaggctgtc agcaatggtc agcgactgca ccatatcgaa catggtgtgg
    41341 tcgacatagc ggtcaccgct tttgctcacg ccaccaatca agtccgaggc gttctgggtt
    41401 tgtgccagat attttgccgc agtactggtt gcacctttgg ttgagagcgc aaacaccgag
    41461 gaaccgctcg gctggcgact ggtaaaggcg tcggcatgga tagagacaaa caggtcagca
    41521 cgctgtttct gggcttttgc tacgcgcact tgcaacggaa tgaagatgtc ttcattgcgc
    41581 gtcatgtaca ccttcatatt gccctctttc tcgatcagag agcgcaaacg gcgagctatt
    41641 tgcaatacca catctttttc gcgcgttttg tatttcccca ccgcaccgga gtcttcgcca
    41701 ccgtggccag ggtcaagcat aatgacaatc ggacgatcgc gccctgcttt acctggttgt
    41761 ggaccacttt gcgctggcgg cacctgcttt tcgaggtcgc ctttgttgta atcctccagc
    41821 agcgccagca gcgggtcctg catatcctgt gcattggccg gatagaggtc catcaccaga
    41881 cgctctttaa acccggcgac cggcgcaagg gcaaacagct gcggttttac gttttgcttt
    41941 aattcaaaaa ccatccgtac ggtttgcggg tcaaattgcc cgacgcgcgc cgacttgatg
    42001 aacgggtcgt ctgcacgaat ttgtgccgcc atccccttga gtaccgagtt caggtttaca
    42061 tcttcgatat ccaccaccac gcgttcaggg ttactcaacg cgaactgctt atatttcagc
    42121 tgacgatttg attctaccgt cacgcgggtg tagctggacg caggccagac gcgcaccgcc
    42181 acgacctggc taaccgcagc caggctgacc tgacttacgc tcaatagcca catagcgccc
    42241 gcgccttgca gtaaacgacg acggctgatt gcagtgttgg atcctgacat gcctctcccg
    42301 agcaaaagaa atctaagctg tgtaacaagt aaacgactaa tttgaccgga aactttagcg
    42361 aaagacgcat aatctgtcat ctaataaacg gtaaacattc tttttatatt cacggcatta
    42421 ctgataaaaa agtcgctctc gcataaaatt tacacttgca ccctgcgaaa aaacagaata
    42481 aaaatacaat aatttcgaat aatcatgcaa aggggtgtgc cgtggtaaag gaacgtaaaa
    42541 ccgagctggt cgagggattc cgccattcgg ttccctatat caatacccac cggggaaaaa
    42601 cgtttgtcat catgctcggc ggtgaagcca ttgagcatga gaatttctcc agtatcgtta
    42661 atgatatcgg gttgttgcac agcctcggca tccgtctggt ggtggtctat ggcgcacgtc
    42721 cgcagatcga cgcaaatctg gctgcgcatc accacgaacc gctgtatcac aagaatatac
    42781 gtgtgaccga cgccaaaaca ctggaactgg tgaagcaggc tgcgggaaca ttgcaactgg
    42841 atattactgc tcgcctgtcg atgagtctca ataacacgcc gctgcagggc gcgcatatca
    42901 acgtcgtcag tggcaatttt attattgccc agccgctggg cgtcgatgac ggcgtggatt
    42961 actgccatag cgggcgtatc cggcggattg atgaagacgc gatccgtcgt caactggaca
    43021 gcggtgcaat agtgctaatg gggccggtcg ctgtttcagt cactggcgag agctttaatc
    43081 tgacctcgga agagattgcc actcaactgg ccatcaaact gaaagctgag aagatgattg
    43141 gtttttgctc ttcacagggc gtcactaatg acgacggtga tattgtctca gaacttttcc
    43201 ctaacgaagc gcaagcgcgg gtagaagccc aggaagagaa aggcgattac aactccggta
    43261 cggtgcgctt tttgcgtggc gcagtgaaag cctgccgcag cggcgtgcgt cgctgtcatt
    43321 taatcagtta tcaggaagat ggcgcgctgt tgcaagagtt gttctcacgc gacggtatcg
    43381 gtacgcagat tgtgatggaa agcgccgagc agattcgtcg cgcaacaatc aacgatattg
    43441 gcggtattct ggagttgatt cgcccgctgg agcagcaagg tattctggtg cgccgttctc
    43501 gcgagcagct ggagatggaa atcgacaaat tcaccattat tcagctcgat aacacgacta
    43561 ttgcctgcgc cgcgctctat ccgttcccgg aagagaagat tggggaaatg gcctgtgtgg
    43621 cagttcaccc ggattaccgc agttcatcac ggggcgaggt tctgctggaa cgcattgccg
    43681 ctcaggcgaa gcagagcggc ttaagcaaat tgtttgtgct gaccacgcgc agtattcact
    43741 ggttccagga acgtggattt accccggtgg atattgattt actgcccgag agcaaaaagc
    43801 agttgtacaa ctaccagcgt aaatccaaag tgttgatggc ggatttaggg taagcgatga
    43861 aaatcgccgg atgcggcagg cgtaacactc gtgcgtcgca tccggcaatt acgtttatcc
    43921 ccgcgaacta aacaacgccg ccagaccact gcgccgctca gtacgagtgg cgattgccgc
    43981 acttaatata cgctcatcgg catacagcga cagacggcga cgcgcgcggg tcaccgcggt
    44041 gtataccagc tctcgcgtta ctatcggcgt gcgttggctc ggtaaaatca acgccgcatg
    44101 gtcgaactcc gatccctgcg atttatgtac cgtcatcgcc cacgtagttt cgtgctctgg
    44161 caagcgactc ggttgcacag acttaatatt gccgtccggc atcgcaaacc agacgcgcgt
    44221 cccctgcccg cgatccagcg caataccgat atcgccatta aacaacccaa gcgcgctgtc
    44281 attacgggca atcatcaccg gtcgaccttc gtaccaacga gagtgcgaat gacgatgaat
    44341 tttgcgcttc tgttgcataa actgctcaat tcgctcattc agtccagcca cgccaaatgg
    44401 cccttcccgc agggcgcaca aaagttggta ctcattgaac gcctgaatga ttaaatccgg
    44461 ctcggcacgc gcttgcagca gatccagata acgtccataa cccgcaagag cttcctcaag
    44521 catcgcaata taatcttcgc cgctctgtaa aagccgtttt tcaatatcag taaaatcctg
    44581 ctgaaaaacg gttttcactg ccgttttatc accacggttg atcgccgcag ctaactgacc
    44641 aatgccagaa tcgctgccga aacgatagct tttttgcagc aggcagagac tgtcgcgcaa
    44701 agatgccgct tctgtgccgg ttcctgccgg aacgtgagtt cccgtcaggc ggcttagctg
    44761 cccggcacgc tcggcggtaa agccggcatt ggcataagcg cagatatcgc ccagcacagc
    44821 cccagcctca accgaggcca gttgatcacg atcgccgaga aagatcactc gcgcatgatc
    44881 gggcaaggcg tcgatcagtc tcgacatcat aggcagatcg atcattgacg cttcatctac
    44941 caccagcaca tcaagatgca gcgggttacc ggcatgatga cgtaaacgct ggctacccgg
    45001 ctgcgcgccc agcaatcggt gcaaagtgct ggcatcttcc ggaatgcgtt tcttttgttc
    45061 atcggtcagc ggtaactgtc gcaaagcctt gccgagagat tcggttaagc gcgcggcagc
    45121 tttacccgtt ggtgcagcca gacggatacg gcagcgttcg ccgtcggcca tttgaattaa
    45181 cgctgccagc aacttcgcca cggtggtcgt tttaccggtg cctgggccgc cggaaatcac
    45241 cgagatccgc cgcgtcagcg ccactgctgc cgccactttt tgccagttaa tttcatcgct
    45301 tactggaaaa agtttgtcca gggtttgcgc cagtagagct tcatcaacct caatggcatg
    45361 attcacctca ttgaagaagc gcgctaccgt ccgctcgtta caccacatgc gattcaaata
    45421 aagacgatcg ccacagagga tcatcggcgt gggttcatct cccctgctga ccgcttgaga
    45481 agccagcaag gattcttccc aattttgtag ctcaccgatt tcactgacac aggtcgccaa
    45541 cagcggatgc gacgcctcgt tattttccag tcgtgaaagc ggcaaacaga cgtgtccctc
    45601 tccggcatca tgacttaaca gtgccgccgc gagggtgacg gcaggatgtt catctcccgc
    45661 cacggtcagg gcaaattgca catccagcgg gcgtagctgt ttgtgctcca cagcttccag
    45721 taattgcttt tgcaatttca ttacgcctcc tccagggtca taccggcaaa catctcatcc
    45781 atcagggcaa tcaacccggc gttgggtcgg gttgtgtaaa tcccttgttg cggatgttct
    45841 ttatcaacgc cacgcaggaa cagatagata acgccgccaa agtgacgatc atagtcgtaa
    45901 tcagcaatgc gatggcgcag atagcgatgc agcgccaggg tataaagctg atattgcaga
    45961 tcatagcggt gtgcctgcat cgctgctgcc atagcctgtt gggtgtaagc cgaactgtct
    46021 tcacccagcc agttggattt atagtcgagc aggtaataac gcccttcgtg gcggaatacc
    46081 aggtcgataa agccttttaa catgccgcgt acctgcatga actccagcgg cgggcagcca
    46141 gcggatagcg ggtcaaactg gcgaattagt gcatcaagct ggctggcgat gagcggttcg
    46201 ctaatcggca ggtaaaactc catctccacc tgtttatcgc ggtcggaaag ctgactcagg
    46261 ctaacacccg tttcattgag aggtgcctgg aggacagccg tgatccactc ggtcaacacc
    46321 ggttcccact gcgattcaaa gccgccgagt tccagttttt cctgtaccca gttcgggtca
    46381 accggctggg taaaatcgag gtcttcaaac aaactgtgca agaatgtccc cggtgacgca
    46441 ccgcgcggga actgatgcgg tgttaacgtc ggttcttcaa cgacgctgac cacgcctgcg
    46501 gcatcaacat ccagccgtgg catcagatcc tgagcgatac cgtgaccacg ctgctgcaag
    46561 ccggagtagc tggtgacacg ccagttatcg ccgggcaatc gttgtagtgt cctcgcattc
    46621 agttctgcag ttaaagcatc attaacctgc cagggctggt tatcaccagt ttgtgccgtt
    46681 tgccaggcaa tatcatcatc gcataacgct tcaatacagg tgcgaagccc tgccgcatct
    46741 tgcggttccc ctttttgcag caaacgcccg agcgcacttt ggtggacgtc ggtgtcacct
    46801 tttttatcgc cacgacggcg caccagcggt gcaacgccga gactgcaatg ccaaaccgaa
    46861 cgcgtcagcg ccacgtaaag caaacgcaga tcttccgcca gacgttcgac ctccgcgagg
    46921 tcgacgcttt ctggcgcagc attaagatcc aaaactgcct caaacgagtg gcgatcgtga
    46981 taaaacgcct gatcctggac gcggaaattg gtaataaatg gcaaccagac cagcggatat
    47041 tccagccctt tcgatttatg gatcgtgaca atctgcacca gatgtttatc actttcgagg
    47101 cgcatttgtt ggctggaggc attactgtct ggctcgagga tatgttgcga taaccagcgt
    47161 accagcgcat gttcactttc cagctgcgtt ccggcttctt gaagcagttc gctgatatgc
    47221 aagatatcgg taagacgccg ctcaccgcct gccgttgcca gcaagttttc tgcaatgtta
    47281 cgcgccgaca tcagcgcccg cagcatcggc ataacgccac gtttgcgcca gatttgccga
    47341 taaccatcga actcttcgac taccgcatcc cacgcatgtt cgtcattatt cagcgtttca
    47401 atatccagcg cattcagccc catcattgac gttgccagcg cactgcgcag ggtgttctca
    47461 cgttcgggcg tcatcaccgc ctgcaacagc caaagcattt cctgcgcttc cagagtttca
    47521 aaaacactat cgcggttcga aaggtaaacg gaagggattt ccagcaacgt taaggcatcg
    47581 cgcacctggg cggcctcctg gcggctgcgc accagcacgc tgatgtccga agcacgcacc
    47641 ggacgcgcgt cgtcgccgtt catcagcaac gcttcgcccc gctgtccggc ttgtagccag
    47701 tcgcggattt gcgcagcaca tacctgcgcc atggtacttt gatagtcgcc aacgccgcag
    47761 ctttcgcctt ccatcagcca cattttcatc gcaggctgcg tttcaccttt aaagacaaaa
    47821 cgtaacgcct gatttttccc ggctgatttc actggaataa acggtatttc gcgaaacatg
    47881 aacgcgtcat cagtctggct gaaaagcttg ttcacactgt tcaccattcc aggtgcggag
    47941 cgccagttgg tgtctaaagt gtagtgggcg tgaacttcac tacgcgcctt catataagtg
    48001 aagatatccg caccccggaa tgcatatatg gcctgcttcg ggtcgccaat cagcaacaat
    48061 gcggtttctg gctgatggtg ccagatacgg cgaaaaattc ggtactgctg ggggtcggta
    48121 tcctgaaatt catcgatcat tgccaccggg aatcgcgtac ggatcgccgc tgccaacacc
    48181 tcgccgcttt cgctacgcag cgcggaatcg agccgactta acatgtcatc aaaacccaat
    48241 tcgccacggc ggcgtttttc acgcgctact gtttcgcgga tctcagccaa tgcgcgggtg
    48301 atcaccagat cacggatcga caatggttct gcaagcagtt gatcgatcgc ctcaaacagt
    48361 ggatgtcgcg gggtttcccc cccggctttc gtgcgatctt ctaagaaacg ctgggagaat
    48421 ttttccagcg actccggcaa ctgataactg tttgtctctt cttctgccca ggcgctgatc
    48481 ttgtcgatcc atttagcctg attgctacgg ttaaacttgc gtcgatcaat accagaagat
    48541 tcgatcagcg catccagttc acccactgcg tcgcgccact gctgttttac cgtatcaata
    48601 cgcgccacaa tttgcgcgtg acgggaagct agcgtttcat catcgggcgg cggtgctttg
    48661 ataaccggcg cttcgccttg cagataacga ttaatatcgc gcagcaacgc ctgcggccct
    48721 ttccaggttt caaagacgac ctgggctatt tcacgcggca gcgggtagca gtggcgacgc
    48781 cagaaatcgg cgcaggcctg gtagcgtagc agagactcat cttcaatcag ctgctgctca
    48841 aacagcatgc cggattcaaa ggcattcagg ttgagcatgc gctggcaaaa gccgtgaata
    48901 gtaaagactg ccgcttcatc catctgccgt tcggctaaca acaaccactg cgcggcttgc
    48961 gctttatcgt cgacctcttc cagcaggtgt tcgtacaatg gattgtcggt ggtttcacgc
    49021 agacaggcga tgcgcaactc gtggatattg ctacggatac gaccgcgcaa ttctgccgtg
    49081 gcagcctcgg taaaggtgac caccagcagt tcttcaacgg tcagcgggcg gggaaaggcg
    49141 gcggaaccgc ctagtccaag taacaggcgc aaataaagcg ccgcaatcgt aaaggttttg
    49201 cctgtgccgg cagaggcttc aatcaggcgc tcgccctgta agggcaagcg caaaggatct
    49261 agtgtctcgg cgacatcact cattcttttc actcatcagg ggcattgttt gctgcaacgc
    49321 gctgacgttc tcccacactt tccagccttc aggatgcaca tattcggctt tcccgttttg
    49381 gctgccggaa atctgcgaca ggatagccat gccttgcggc tcgactaccg cctgatggaa
    49441 gaaatcggcc agtttttgcg gcgtcagcag tttaatctgg gccacgattt tatcacgcga
    49501 atcgaagcgc atattgccgc gatcgaaatc tttacttaac ttcgatgctt cttcgccgag
    49561 cgtttgcggt gcctgcagca tctgggtaat taccgcctgc tggatttgcg caaactcatc
    49621 tggcttcatc gctcgcaatt ttgcctctgc ggttgggaaa aacgccttgt aacgctccca
    49681 caagaatgaa ggctgtttat cattgctttg caaaaggaag cccatgcccc actgacgccc
    49741 cacgctcatt ggaaacgcaa acacggcata gcccaattgt tcttcggtac gcaactgatt
    49801 gtagaaccac ggctgtacga tctgccccaa cagagagcta taggctgagc tggtgtattc
    49861 atcgtagcca gtcggtacaa ataccgctgc cagtgcggag tcggtgctgt taccggcttt
    49921 ttcaaagatg acggattgtt ttttatcgac cactacatct ttgtttcgac accactctga
    49981 accatcagcg cccaactgtt tttgcacatc gcgtgccagc gttgttgcct gggcctcggt
    50041 catgttgccg ataaccataa actctggtcg agcccctgat tttaaggcgt cgcgataagc
    50101 cagcacctct ttcaacgtaa tggagggcaa aattttacgc cgttcatctc gcgagaagta
    50161 cggcacttgc gagagcatct gcgcgggcat aatcacctgc tcaaacgctt tacccttttc
    50221 tgcggaatcc atcatctggt tataccagga cttcgcctgc tcaagctgat cttccgtagc
    50281 ggtatagcta aagtacccct cgagcaatgc ctggaacagc tgcggcagac gctgggtgta
    50341 accattagca ttaaccataa ggccgttgtt agcgttggtg gaaaaactta tgccaccaac
    50401 cgacgcctgg ttgcttaact gatcaagcgc cagccctgcg agataatcat tgagcgcaaa
    50461 catcacctga ttgcgggcgc tgtccatggc tttcggatta cgcaaaatca ggctgacatc
    50521 agctttgggt tcgctggaaa aataacggct tggcgcatac accacgcgca gattcgactc
    50581 atcaacaatc agctctggat ggtcatattt cttctctgac ttaatcagcg agaaatcgtc
    50641 aggaatatag gggttaagct ccggtaagga gagcgcaata ttggcagctt tttgctgcca
    50701 gtcagcgaaa gtttgttcgc tgattttatc gacctgatac ggcgcatcga caaaataagc
    50761 cgttttattg tgcggctctt tcgggctgat ataccagata cgcgcattct gcggcgtcat
    50821 catcgccaga cgttctttta ctgctttagc atcgtaccga tcggcaatat tgactgcatc
    50881 cagcgtatgc tcaaccggaa cgcgaatcat ggtatctgcc agccattcga cgtaatccat
    50941 atcacgggtg atcgacggat aacggaagtc gatatccagc acattcgcca gttcatcgaa
    51001 gtattgttta tcaatgcctt tttcacgtaa cagattgaga tagctaaaaa ttgccgccac
    51061 aacctgatcg cgattagcca ggcctttatc ggttaaagac gcagagatcg ctaatacgcc
    51121 gctgttgccg ttgacgatag gatcggagtt ggcgctaatg ccctcaacta atccctgctt
    51181 ttgcagccag tcggaaagtg tacctgggct gcgattacca atcagatagg taatcaattc
    51241 atccgtttta ctacggaact tcgctgagtt gttatcgatg cgaaactcaa cgcgcagaac
    51301 tttacgcggc attgccggga cgtaatgaat gataatgccc ttttgcgcgt cggtgactac
    51361 cggcacggtg atttccggtt ttttgctctc tttgttcggc acgcgaccaa aggtgtccgc
    51421 cgccattttt gccaactccg gcagcggttt attgctgtaa ataaccgcct tcatcagatt
    51481 ggcggaatag tacttctcgt ggaaatcttt cagcgcctgc tgcaccggat taccaggttt
    51541 atcgcttaaa gtttcgaggt taccaccaga aaactttgaa ccggggtgtg ccgggttaat
    51601 ggtttctgcg ctgacctgtg ccatgcgcat cccgtcacgc gtacgcgcca tggttaattc
    51661 agcgttcacc gcattacgct cacgttcggc atatttcttg tcgagcaaag gttcagcaat
    51721 agcatcggcc aggcggtcta ccgcaccagg caaggcgtcg ttctcaactt ccagatagaa
    51781 agccgtgcga tacggcgcag tgctggcatt gtgactaccg ccgtgcattt tgagatattc
    51841 ggccagactg tcagcctgcg ggtacttttt cgaccccatc agactcatat gttcaaggta
    51901 atgtgccagc ccctggtacg cctcgggatc ttccagcgac ccaacgggca ccaccagcgc
    51961 cgagagcgat ttaactgcct gcggatcaga aaccagcaag accaccatac cgttatccag
    52021 acgtatagcc tgatactggc ggttatcttt atcactttta cggatggttt cctgaatcgg
    52081 ctgccatccc gtttctgcct gacttaaggg tgcccaaagg gcaactaaca acaataatgc
    52141 tttgaaccag gtgctgcggg gcattctcgg acctcataat caacttaatt ttctgtccag
    52201 attcaacacg ttaacgcaaa tcatctgcca gaatttaatc ttgtgctgca cgagtcagcc
    52261 tatgtttata taaccatcag tccgtgactg gtgcgcatca taaagtaagc ggatagattg
    52321 cgcaattttt atacagcact catgactgat taaagcgaaa cagcggtaac aggaaacgtt
    52381 gcgactgttc aacgatggcc tccattgtct ctggtgttaa ttgccgccag agcctttgat
    52441 accagatatc atcaccttcg ccacgcacca tcatgttacc ttcgtaagcc tgaaggaatt
    52501 tcgtacgggc tttttgcaac gtggaatcgt catccagcat ggcatcgttt tgcgcgtcat
    52561 aacaggtttt tagccacgcg ccgccacttt caggtaacac cagcaatggc gcggacatcc
    52621 cttcacgata cccctcaatc agttgtgaga ggtaatgcaa agcctgttcg gctgcaagcg
    52681 gcggaaaacg ccactcgccg tctttgcgta gaaaaaggcg actttcacca ttaccaccgc
    52741 tggcacagta gacaaggtgt tccagccaaa gttgcattcc ctgcgccaca cttaataaag
    52801 agggacgcca gcgcaacagg ccatccggct gcacctgcgg caaccagcca gttatctgca
    52861 caccgttgca ggcgagatca atttccatac tctgccccgg ctggcgacag gcaatgactc
    52921 tgtcggcaag ctgctgcatc tcctggcact gagtttccca gaaaatttca ccaaaagcgc
    52981 catacggtaa atcccctgcc gcccggaagc gacggaacaa gcgttcggca tcatcctgct
    53041 caaccagtgc attcaataac tgctgattga tttgataacg gctaagccct tccagaataa
    53101 atggctcggt gtcggggatt tcgctgtctt cagtacggaa gttcacctgc aaacgcatct
    53161 ggaagaacgc ccgcaccgga tgtgcccaga atcgttgtag cgtttccagc ggcacggttt
    53221 ccggtaaggt aaacggcagc ggctgaacaa attcagaatg tgctttacca gcctggctgg
    53281 ccgcaggtag ccattcacga gcatagcttt gtcgttcgcc tggctgatag ttttgtggat
    53341 caaacggcat tcgggtatgg aggcaagtaa gatgcgcttt tacccttgcc tcgctttcat
    53401 cacagttgag cgcttcatcg cccggtagat aatgactttg cccgatgtag tcgatcagtt
    53461 cctgcaccag taccgacggg aaacgttcac tgttatcctg aatggaacgc ccgatatagc
    53521 tgatatagag tttttgctgc gcggaaatta acgcttccag gaacagatag cggtcgtcat
    53581 cgcgacggct acggtcgcca cgcttcggtt tctggctcat caggtcaaag cccaatggcg
    53641 caagctgacg tggataaacg ccgtcgttca ttcccagcag gcaaaccact ttgaacggaa
    53701 ttgaacgcat tggcatcaga gtacaaatgt taaccggtcc ggcgagaaaa cgctggctga
    53761 tacgttcttg atccagacgc tgtgccagtt catcacgcaa tagtgacagc ggcaccgcgt
    53821 cgccatactg cgcacctaaa ccttcggcga taatcgcctg ccattgttgt tcgatcagcg
    53881 tcatcgccgc ttcggtttcc gcatccggca ggaagaaggc gctgagcata tcgcgacaaa
    53941 ccggcaacca ctcttctagc ggacgctcct gtgccagccc gcgacgccag atgtttagct
    54001 gcattagcag tgaagccaga tgccccacca gttctgcaat taagccgctc gattcatcat
    54061 aaggtagaac cgattgccac tcgccctgcg cgctctccat cgcgtagccc agcaacatac
    54121 gcgtcaggcc aaatcgccag gtgtgttgtc cggtggcggg gagttccagc tcgcgaacgt
    54181 tgtcgtcatc tatcccccaa cgaatgccgg attcgttgac ccactggcgt aaataacgca
    54241 gcccttcttc ggtgatgtca aaccgcgccg ccagcaccgg cacatccagt aatgccagca
    54301 cgtcttccga cacaaagcgg ctgtctggca acgataacag gctgataaac gcttccagta
    54361 ccggatgcga ctgccgcgcc cgacggtcgg aaatggcgta aggcaggtaa cgatccgcag
    54421 gtgcgctacc aaacacagcc cgaataaacg gactgtagct gtcgatatca gccaccatca
    54481 cgatgatgtc gcgcggagta agtgtcgggt cttcttccag catcgccagc aggcgatcgt
    54541 gcaaaacttc aacttcacgc tgcgggctat ggcaaacgtg gaaggtgata ctgctatcca
    54601 gtggatcaag cgggcgtttg ttatcgctac gggaaaactc ttcgatgttc acaccagcaa
    54661 cggcacggtt ttccagttcc agaatgtcag actgaatgtt atgcagcagg ttatctggcg
    54721 tgacatcgac aaaggcgtcc agctcctggc tgctctccag gtcagaaagg agataaatgt
    54781 agtcgcgccc aagcttaccc catgaagcca gcagcgggtt gccgacatcc tgttcaccat
    54841 cgctgttaaa gagctgcccg gcattttcgc tatcacgaaa tagcggcaat tcgcgatctt
    54901 caaaactgtg tcgacgctga cgggtcagca gtttcgccag ataagcagga tctttaatat
    54961 cgccccagta ataacggcag gggttggtaa acaggagatg gatttcaata tgtttaccca
    55021 gcgcctgtag cgcctggaga taaacaggcg gtaacgcgga aataccgcat ataaagacgc
    55081 gcgaaggtaa ccccggcggg caggtcgtcg cggactccag cgtttcgata aagcgctgat
    55141 agagattggc gcggtgccag cgcggttgcc cgagttgatg ggtatattcc accagcgcct
    55201 tccacaacgg ggcttgccag gcctgtgctt ctcccagccc ttcaaccaaa tgtcctgttt
    55261 cccactgtgc cagccagtcc ggacgataga ccagatactg gtcaaacagg tccgccgctt
    55321 ttgaggaaag ctggaacagt tttcgcttgt cgctatcgtc agtcagataa tgccgcaaca
    55381 gggtaaagtc ttcgcgctcc agcaattgcg gcagcagagt catcagtttc cagctcatgc
    55441 tctgtttgtt aaaggcgctc tctttgggga tttccggtaa cacccggacg aacatatccc
    55501 agataaagct cgctggcagc ggaaaatcaa tgtttgccgc aatactaaac ttttgcgaca
    55561 gggtcatttg cagccactgc gccataccgg tactttgcac cagaatcatc tctggttcga
    55621 aaggatcgtc cagccgttcg cgttcgacaa taaactccat caacgcttcc agcacgtcca
    55681 gacgattgga atggtagacc cttaacatag cggctcctga ctactgacga ttcgggcaat
    55741 gcaggcgggt catctcgcct tctctgcccc cgggtgaaac tagcgtaacg ctgatgctga
    55801 cacatcccgc ctgcgatgtc tgcattcggt tgacctgcca gttggcaggt ggcgaaatcg
    55861 cgcgcagttg cgtttgctgc caaccatgcc gccagagctg ttggtactgg tttctgctgg
    55921 cgagactgtt cattaatgtt cgctggatac ccgataacgc agtgacaatc atcaccatca
    55981 gcaccatcgc aaccattacc tccggcaggc taaagccttg ttgattcttc agggaagctg
    56041 acataacgcc ctctctttca gcggacaaaa atcgctccag ccgcgtggcg aaaaaacaat
    56101 gtttccatca atgacttcgc ctgttcgcca caacgaaacg ccttcataac cggcaaccaa
    56161 taaggcttca ttatctgcca gtaaacgcaa acaaacccgg gcatcggttc cagcgtactg
    56221 cgagcactga actgcgggct gcgtctgcca ggagtgcatt ttcccccacg ccagcgccga
    56281 ctgaacgatg gcctggcggc gcaatgactg actttctatg ctcacgcgag aggcaaaact
    56341 gcgatcctgc tgactcattc cttgcaatag caagctaccc aaaaccagca gcatcaggac
    56401 cagagccagt gacgaaacac ctttttcgcg gttcacaggt tgaatcctgt cacgctatag
    56461 ctggcatcca ccacggtttg cggttcagac ttactggcag cacgcatatt aaccgtcaac
    56521 accggcgaga agccgctgac atcctgacgt acgacctgaa aagtgtcgat aatgatggca
    56581 tccggattag tgactttatc ccagccctta ccttcacagg atgtcgcacc gcgtagcgtt
    56641 tccagcacat gctccttcag acgaaatcca atctggtcag actcttttac cggttcgcga
    56701 tcccagatac cgttactgtt cgcatcccac tgcacaatga tacagtcacc ctgtccgaca
    56761 atttccagcc cttcgccggt acagctgcca tgacaataac ccgccctctg gagatgcttc
    56821 gcgacggtaa atacccgcag ccagatttca tcttccagcg ccagcttacg ggtgctcgtt
    56881 aaactttcac gctgtaacgc aggcagaaag cgtgcagccc ccagcaacaa tacgctactg
    56941 atcgccatag caatcaacac ttccagcaga gaaaaacctt gctcttttac aggcatcctt
    57001 ctgtttctcc ttgctgacaa agccggagtc ttccccacgg cgaaaccacc agccaccact
    57061 cgcccgttga gtttttgaag cgaatatgcc cggcccatgc ggtattgcgc aggccaaaga
    57121 aagcaagcga aggtgtcagg tcgctcattt cgacttcggg ccagcgtggc acaaagacca
    57181 atggtgaact gccatgacag gtattggccc cagcagcgga actcacaagg caccataacg
    57241 tcccctccct gataacgctg atactgtggt cgcggttatg ccagttggca tcttcacgta
    57301 aatagagcaa atagtcccgc gcctggctgg cggtttgcca tagccgttgc gactgctgcc
    57361 agtattgcca gccatagagt ccacttgcgc ttagcatgac caaaatcagc atcgcgacca
    57421 gcgtttcaat cagcgtataa ccacgttgtg ttttcatgcc ggcagtatgg agcgaggaga
    57481 aaaaaagacg agggccagtt tctatttctt cggcgcatct tccggactat ttacgccgtt
    57541 gcaggacgtt gcaaaatttc gggaaggcgt ctcgaagaat ttaacggagg gtaaaaaaaa
    57601 ccgacgcaca ctggcgtcgg ctctggcagg atgtttcgta attagatagc caccggcgct
    57661 ttaatgcccg gatgtggatc gtagccttca atctcaaagt cttcgaaacg gtagtcgaag
    57721 atggattcgg gtttacgttt gataatcaac ttcggcagcg gacgcggttc gcggcttaat
    57781 tgcagatgag tttgatccat atgattgctg tacaggtgcg tatcgccacc ggtccagaca
    57841 aaatcaccca cttccagatc gcactgctgc gccatcatat gcaccagtaa cgcgtagctg
    57901 gcaatgttga acggcaggcc gaggaagacg tcacaggagc gctgataaag ctggcaagag
    57961 agtttgccgt ctgccacata gaactggaag aatgcatggc acggtgccag cgccatttta
    58021 tccagttcgc ctacgttcca cgctgaaaca ataatgcggc gggaatccgg gtcgtttttc
    58081 agctggttca gtaccgtagt gatctggtca atatgacgac catctggcgt tggccaggcg
    58141 cgccactgtt taccatacac tggtccgagg tcgccgtttt catcggccca ttcgtcccag
    58201 atggtgacat tgttttcgtg tagataagca atgttagtgt cgccctgcag aaaccacagc
    58261 agttcgtgga tgatggaacg caggtggcaa cgtttagttg tcaccagcgg gaatccatcc
    58321 tgcaggttaa aacgcatctg atgaccaaaa atggaaagcg ttccggttcc ggtacggtcg
    58381 tttttctgtg tgccttcgtc gagcactttt tgcatcagtt ctaaatactg tttcatggtt
    58441 cctcaggaaa cgtgttgctg tgggctgcga cgatatgccc agaccatcat gatcacaccc
    58501 gcgacaatca tcgggatgga aagaatttgc cccatgctga tgtactgcac ccaggcaccg
    58561 gtaaactgcg cgtcgggctg gcggaaaaac tcaacaatga tgcgaaacgc gccgtagcca
    58621 atcaggaaca agccagagac agctcccatt gggcgcggtt tacgaatata caggttgagg
    58681 ataataaaca gcaccacacc ttccagcagc agctcgtaaa gctgtgatgg gtggcgcggc
    58741 agcacaccgt aagtgtcgaa aatggattgc cactgcgggt tggtttgcag cagcaaaata
    58801 tcttctgtac gggagcctgg gaacagcatg gcaaacggga agttcgggtc aacgcggccc
    58861 cacaattcac cgttaataaa gttgcccaga cgcccggcac caagaccaaa cggaatgagt
    58921 ggtgcgataa aatcagaaac ctggaagaag gaacgtttag tacggcgggc aaagataatc
    58981 atcaccacga taacgccaat caggccgccg tggaaagaca tgccgccgtc ccagacacgg
    59041 aacagataca gcggatcggc cataaactgc gggaaattgt agaacagaac ataaccaata
    59101 cgtcccccga ggaagacgcc gaggaagccc gcatagagta agttttcaac ttcatttttg
    59161 gtccagccgc tgcccggacg attcgcccgt cgtgttgcca gccacattgc aaaaatgaaa
    59221 cccaccagat acatcaggcc gtaccagtga agcgccacgg gtcctattga gaaaatgacc
    59281 ggatcaaact ccggaaaatg cagatagcta ctggtcatct gtcaccacaa gttcttgtta
    59341 tttcgctgaa agagaacagc gattgaaatg cgcgccgcag gtttcaggcg ctccaaaggt
    59401 gcgaataata gcacaagggg acctggctgg ttgccggata ccgttaaaag atatgtatat
    59461 gatccgcgct ataaccctcc gcgaatcagc ccgcccatgc cgcgacgctc cataaaggct
    59521 gcaacctgat ggcgaacttc ggtcgccagt tgcgcttcca gactacgctg cgcaagattt
    59581 tctgcttcgg cataatcaat gcgccgcagc aggtatttta cccgcgctac agaacgtccg
    59641 ttcatagaca gatggcgata cccaagccca atgaggattg ccacgcacat gggatcgccc
    59701 gccatttcac cgcacaaacg gagatcgatt ccatgtattt ccgcttcccg ggcgatcatc
    59761 gccagagctc gtaacattgc aggatgaaga ctgtcataaa tgttcgccac ccgggtattg
    59821 ttgcgatcaa cggccagaat gtattgagtc agatcgttgg tgccaacaga gatgaaatcg
    59881 acccgctttg ccagatgcgg cagcataaat accattgacg gcacttccag catgatgccg
    59941 atacgtggtt tgggaatttc gtaaccgatc atctcctcga cttcacgtcc ggcacgttca
    60001 atcaggcggc gtgcttcatc aacttcatcg aggcttgtga ccatcggcaa cagaatattc
    60061 aggttgcccg tagcggcatt agcacgcagc atcgcccgca cctggatcaa gaagatctcc
    60121 ggctgatcga gcgtaatgcg aatcccacgc caacccaggc atggattctc ttcgctgatc
    60181 ggcatgtaag gcagctgctt atctgctccg acatccagcg tacgcaaggt gacgggttta
    60241 tcattgaaca tttgcagcat cccctgatac tgcgccacct gttcttcttc cgacgggaaa
    60301 ccactttgca gcatgaatgg gatttcagtg cgataaagac cgatgccatc aatacggctg
    60361 cccagttttt cttcatgttc cgggcttaaa ccagcattga gcatgacttt tatacgctca
    60421 ccgcttttta actgcgcggg taaattgacg tcatcttccg ccagacggct aagctcaatc
    60481 tcttcactaa ttagccgctg atattcttgc agcagtaccg gctccggatc gaccagcaat
    60541 tcaccgcgat agccatcaac gatcagcgtc ctacgatgca gcaccgaagg ctgaatatcc
    60601 gcgcccatca cggtagggat ccccagcgca cgtaccatga tcgcagcatg ggagttggct
    60661 gcgccatctc gcacgacaac accgactaag cgatcctggg gcagctcagc aagagttgtc
    60721 gctgacagtt catctgccac cagaatgaaa cgttccggcc aggcgttcgg cccttgatta
    60781 gcgtcatcaa gatgaaacag caatcgctga cccagcgcac gtaaatcgcc agcccgctct
    60841 ttgagatagt tatcgcttag cgcggcaaac tgttcggcaa atttttcaat gaccgttttt
    60901 accgcccact ctgccaccga gcctttatca acctcggcaa acaattcgcg acgcagccgg
    60961 gtgtccgaaa gtaggtgcga gtaaagatcg aaaatagccg ccgtttcttt ttgtgctccg
    61021 gcggcaaagc gtttgctgta gcggcgaaac tcgtttgccg cttcttccag cgccccggtc
    61081 agtcgttcgc gttccagagc cggatccagc gttgatgcct gatacacctg ttccattaaa
    61141 ggtaacgtgg catcctgcca gccttcggca atcgccacac caggtgctgc cggtaatgcg
    61201 cggattcgcg tctggcgata ttgcccaaac aaggcagtca actgcgactg agaaagaata
    61261 gctgccatct gggtggcaag cgtcaccagg aaggattctt cactttcgtc atactggcgc
    61321 aactctcgtt gctgtaccac cagtacacca agcaactggc gacgttgaat aattggtacg
    61381 cctaaaaacg cgcggaaacg ttcttctttt acggagggga tgtatttgaa gctggggtgc
    61441 ttttgcgcat ctgcaaggtt tatcggttcc gccagcctgc caaccaggcc gacgatccct
    61501 tcatcaaacg cgagcgttac agtgcgaccg cgtggttttt tcagcccccg ggtcgccatc
    61561 aggtagtaac aacgtcgatc atgatcggcc aggtagaccg aacagacctc ggtatccatc
    61621 gcaagacaga tgtcggtaac cagaatattt aacgcctcat tcaggcgtgg tgcgctggct
    61681 accttttcga ctatttcgcg caggcgagtg agcataattg gcgtgactta acctctttta
    61741 cgtcgataag cagatgtgtt ttgtggtttt ggcgtatttt cctgcagtga catcaccaca
    61801 ctcgcgaact ctttcattac cctacggtag acatcacgtt taaatgacac cacctgtctg
    61861 accggatacc agtaacttac ccatcgccag ccgtcaaact ctggtgtact gctggtttgc
    61921 atattgattt ctgcatcgcc gctcaccagc tgcaagagaa accatttttg tttttggccg
    61981 atacaaaccg gcttcgtgtc ccaacgcacc aaacgtttcg gtaatttgta gcgcaaccag
    62041 ttacgcgttg aagcaaggat tcgaacgtct ttgcggctta atcctacttc ttcaaacaat
    62101 tcacggtaca tcgcctgctc tgcggattct ccggggttga ttccgccttg cggaaattgc
    62161 caggagtgct gaccaaatcg ccgggcccac attacctgcc cctggcgatt acaaatcacg
    62221 atacctacgt ttgggcggta gccatcgtca tcaatcaccg gactacctca aaataaagct
    62281 ttatatacga atgattgttt catactccaa gaagacggta aaccactctc tgcagggcat
    62341 tacacactaa taacaattga ataactcaca gttatgtgca gagttataaa cagaggaagg
    62401 ggtggatagc cgtttttatt caccttttct gtggatagag ttgtgaagaa ctacggaatt
    62461 actacgggaa aacccggagc attctgaata agcccgatat agacatttta aaatattcat
    62521 tattagtcaa tgcattacat gtaatttgag taatgaaaaa gagtaattcg tgacccaggt
    62581 cacacctctc atttacgggt tggcgaaaga tcaaccaatg ccgtatttat ccacagaatg
    62641 tgccactaag ttaagcactg aaccactaaa aactggagtt tcgtcgcacg tcaaggctgt
    62701 aaatggaaac agtagtggag gtttttcaca gttatcccag ctttctgtgg ataacatggt
    62761 gtaagatcct gtttattttc agtgaccaga tttggaaaac ctattgcagt gttgcgcaac
    62821 tcgattaccg gcagcctaaa aagcaatata aatcagagaa ttgaacaaca catgtggaaa
    62881 aagttacact gcgaatattc ggcacataat tgctgtttgt tttttaacca aggtatcatg
    62941 acatgtccca acctcgccca ctcctctctc ctcccgaaac tgaagaacag ttgttagcgc
    63001 aagcacagca actttctggt tatacattgg gagaactggc ggcacttgcc gggctggtta
    63061 cgccagagaa tttaaaacgc gataagggct ggattggcgt gttactggag atctggctag
    63121 gtgccagcgc agggagtaaa cctgagcaag attttgctgc tctgggcgtg gaacttaaaa
    63181 ctatccctgt ggatagtctt ggtcgtccgc tggaaacaac attcgtttgt gttgccccgt
    63241 taacgggtaa tagcggggtg acctgggaaa ccagccatgt gcgccacaag ctcaaacgcg
    63301 tactgtggat accggttgaa ggtgagcgca gcatcccgct ggcgcagcgt cgcgtaggat
    63361 caccgttgct gtggagcccg aatgaagagg aagaccgaca gctacgcgaa gactgggaag
    63421 aattaatgga tatgattgtt ctcggtcagg ttgagcggat caccgctcgt cacggggagt
    63481 atttacagat acgaccgaaa gcagcgaatg cgaaagcgct taccgaagcc attggtgccc
    63541 ggggcgaacg gattctgacg ctgccacgcg gcttttattt gaagaagaat ttcaccagtg
    63601 cactactggc ccgtcatttt ctgatccagt agccatcgct ttgacctgcc gctttccggg
    63661 catataatta ccgcttcatt tttttggcag ggctttttag atgttatttg catggataac
    63721 cgatcctaac gcctggcttg cgctcggtac gctgacgctg ctggagatcg ttcttgggat
    63781 cgacaatatt attttccttt ctctggtggt ggcaaagctc cccacagcac aacgtgctca
    63841 tgcgcgccgt ctggggttgg cgggagccat ggttatgcgt ctggcgctgc tggcatcaat
    63901 cgcctgggtt acgcgcctga caaatccgct ttttacaata ttcagtcagg aaatttccgc
    63961 ccgtgatttg attctgcttc tgggtggctt gttccttatc tggaaagcca gcaaggaaat
    64021 ccacgaatcc attgaaggtg aagaagaagg gctgaaaaca cgcgtttcat cattcctcgg
    64081 cgctatcgtg cagattatgc tgctggatat tatcttcagc ctcgactcgg tgattaccgc
    64141 tgtgggtctg tcagatcacc tgtttattat gatggccgcc gtggtgattg ccgtaggcgt
    64201 gatgatgttc gccgcgcgct cgattggtga ttttgtcgaa cgccatcctt cggtaaaaat
    64261 gctggcgctc tctttcctga ttctggtcgg ctttaccctg attctggaaa gtttcgacat
    64321 ccacgtaccg aaaggttaca tctacttcgc gatgttcttc tctattgcgg ttgaaagcct
    64381 caacctgatt cgcaacaaaa agaacccgct ctgatactcc gtacgctctc ctgcgggagg
    64441 gcgtactcac tcctgttacc ctcacagttt aagattttcc tgctttcaag atatataacg
    64501 tcggtttata aacagactat tatcataggt gggccaaatg aggataaccg gatgaaaaaa
    64561 tgggcagtaa taatttctgc agtcggactg gcgtttgctg tttccgggtg ttccagtgat
    64621 tacgtcatgg cgaccaaaga tggccgtatg attttgaccg atggaaaacc tgaaattgat
    64681 gatgataccg ggctggtgag ttatcacgat cagcaaggta acgcgatgca aattaaccgt
    64741 gatgatgttt cgcaaattat tgaacgttaa caaataaggt cagcatccgg ctgtaatgcc
    64801 gatcagttaa g
//
LOCUS       DAEEKQ010000007        63136 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.007, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000007 DAEEKQ010000000
VERSION     DAEEKQ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 63136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63136
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.007"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(1..413)
                     /locus_tag="KLK57_000437"
                     /pseudo
     CDS             complement(1..413)
                     /locus_tag="KLK57_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417214.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
     gene            complement(519..3080)
                     /gene="mutS"
                     /locus_tag="KLK57_000438"
     CDS             complement(519..3080)
                     /gene="mutS"
                     /locus_tag="KLK57_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HBE0890915.1"
                     /translation="MSAIENFDAHTPMMQQYLRLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            3156..3290
                     /locus_tag="KLK57_000439"
     CDS             3156..3290
                     /locus_tag="KLK57_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890916.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            3367..3720
                     /locus_tag="KLK57_000440"
     CDS             3367..3720
                     /locus_tag="KLK57_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HBE0890917.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
     gene            complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="KLK57_000441"
                     /pseudo
     CDS             complement(3757..5834)
                     /gene="flhA"
                     /locus_tag="KLK57_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
     gene            complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="KLK57_000442"
     CDS             complement(5908..6918)
                     /gene="hypE"
                     /locus_tag="KLK57_000442"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HBE0890918.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARAAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEVALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="KLK57_000443"
     CDS             complement(6915..8036)
                     /gene="hypD"
                     /locus_tag="KLK57_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HBE0890919.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQENEA"
     gene            complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="KLK57_000444"
     CDS             complement(8036..8308)
                     /gene="hypC"
                     /locus_tag="KLK57_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HBE0890920.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="KLK57_000445"
     CDS             complement(8299..9171)
                     /gene="hypB"
                     /locus_tag="KLK57_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HBE0890921.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="KLK57_000446"
     CDS             complement(9175..9525)
                     /gene="hypA"
                     /locus_tag="KLK57_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBE0890922.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            9737..10198
                     /gene="hycA"
                     /locus_tag="KLK57_000447"
     CDS             9737..10198
                     /gene="hycA"
                     /locus_tag="KLK57_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HBE0890923.1"
                     /translation="MTIWEISEKADYIAQRHCRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            10323..10934
                     /gene="hycB"
                     /locus_tag="KLK57_000448"
     CDS             10323..10934
                     /gene="hycB"
                     /locus_tag="KLK57_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HBE0890924.1"
                     /translation="MNRFVIVDSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRIDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            10931..12757
                     /gene="hycC"
                     /locus_tag="KLK57_000449"
     CDS             10931..12757
                     /gene="hycC"
                     /locus_tag="KLK57_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HBE0890925.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLVIACWLLWQRYGTLDLCLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            12760..13683
                     /gene="hycD"
                     /locus_tag="KLK57_000450"
     CDS             12760..13683
                     /gene="hycD"
                     /locus_tag="KLK57_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HBE0890926.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWTGFGFAFLAFVSLLAA"
     gene            13701..15398
                     /gene="hycE"
                     /locus_tag="KLK57_000451"
     CDS             13701..15398
                     /gene="hycE"
                     /locus_tag="KLK57_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HBE0890927.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDELYPLRKDSMDYRQRPAPTTD
                     AETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHRGMEK
                     LAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILLEVER
                     LHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIRRDLL
                     KDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEMARDFSNVGPMVRA
                     SGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLDNLPG
                     GPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPTLRYM
                     LRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNSPLK"
     gene            15408..15950
                     /gene="hycF"
                     /locus_tag="KLK57_000452"
     CDS             15408..15950
                     /gene="hycF"
                     /locus_tag="KLK57_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HBE0890928.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVETDLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFALCNCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            15950..16717
                     /gene="hycG"
                     /locus_tag="KLK57_000453"
     CDS             15950..16717
                     /gene="hycG"
                     /locus_tag="KLK57_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HBE0890929.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQQAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            16714..17124
                     /gene="hycH"
                     /locus_tag="KLK57_000454"
     CDS             16714..17124
                     /gene="hycH"
                     /locus_tag="KLK57_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HBE0890930.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQKEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            17117..17587
                     /gene="hycI"
                     /locus_tag="KLK57_000455"
     CDS             17117..17587
                     /gene="hycI"
                     /locus_tag="KLK57_000455"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HBE0890931.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEEE"
     gene            17612..18385
                     /locus_tag="KLK57_000456"
     CDS             17612..18385
                     /locus_tag="KLK57_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001026459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0890932.1"
                     /translation="MNIRTGMCAFLLSLVLPAQATSFTEYLPMSDSEYAQKRALKPLL
                     TMPYDAEQTWRFRKVGVAGVTLEKMPNDDSEWQLNGKDRAGKSWSVPVGMLANIAGKG
                     QLYRADLDRNGIQDLVIWLPSTGNGLAPYAHLILMTFTREGRPCVFEPRGFYTASKTG
                     VDDLLDLQGNGHTQLLDMQFDSGYWITSLYQVKDAKWQRVHGWFGKLSYPALTRFTYT
                     PNRKLVLKPIAGRDPQTEDLAQTQRCLIKGDVLDGVNQN"
     gene            18547..18858
                     /locus_tag="KLK57_000457"
     CDS             18547..18858
                     /locus_tag="KLK57_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HigB family
                     toxin"
                     /protein_id="HBE0890933.1"
                     /translation="MHIISKAPFEESARKYPNDALALQALYRVIKETDFSTPEEMRTA
                     FPNLDNFKYRNKWYVLDVGGNNLRVIAYINFVNKRFFVKHITNHAEYDKLTRYYRENK
                     E"
     gene            18855..19274
                     /locus_tag="KLK57_000458"
     CDS             18855..19274
                     /locus_tag="KLK57_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001696729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0890934.1"
                     /translation="MTANAARAVKATRELVNAVPFLGGSDSEDDYREALELVEYLIEE
                     DDTNPLIDFLASRIAEYENNNQEFAEFDKAVAAMPVGVALLRTLIDQHNLTYADLKNE
                     IGSKSLVSQILSGQRSLTISHIKALSARFGVKPEWFL"
     gene            complement(19388..20812)
                     /gene="ascB"
                     /locus_tag="KLK57_000459"
     CDS             complement(19388..20812)
                     /gene="ascB"
                     /locus_tag="KLK57_000459"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HBE0890935.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGTYPA
                     YSARVFREKGVTIDKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="KLK57_000460"
     CDS             complement(20821..22278)
                     /gene="ascF"
                     /locus_tag="KLK57_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HBE0890936.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRVVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSVQPTVAKEVSLN"
     gene            22538..23548
                     /gene="ascG"
                     /locus_tag="KLK57_000461"
     CDS             22538..23548
                     /gene="ascG"
                     /locus_tag="KLK57_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HBE0890937.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            23696..24223
                     /gene="hydN"
                     /locus_tag="KLK57_000462"
     CDS             23696..24223
                     /gene="hydN"
                     /locus_tag="KLK57_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HBE0890938.1"
                     /translation="MNRFIIADASKCIGCCTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            24376..26628
                     /gene="hypF"
                     /locus_tag="KLK57_000463"
     CDS             24376..26628
                     /gene="hypF"
                     /locus_tag="KLK57_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HBE0890939.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLREDPETLLVQLHQHCPPLARIDSVEREPYIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRARKHRPAKPLAVMLPVADGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPDLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLAAFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(26756..27889)
                     /gene="norW"
                     /locus_tag="KLK57_000464"
     CDS             complement(26756..27889)
                     /gene="norW"
                     /locus_tag="KLK57_000464"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HBE0890940.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDASIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLHLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNDDIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(27886..29325)
                     /gene="norV"
                     /locus_tag="KLK57_000465"
     CDS             complement(27886..29325)
                     /gene="norV"
                     /locus_tag="KLK57_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HBE0890941.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTKSDLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            29512..31026
                     /gene="norR"
                     /locus_tag="KLK57_000466"
     CDS             29512..31026
                     /gene="norR"
                     /locus_tag="KLK57_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HBE0890942.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VMAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            complement(31023..31988)
                     /gene="gutQ"
                     /locus_tag="KLK57_000467"
     CDS             complement(31023..31988)
                     /gene="gutQ"
                     /locus_tag="KLK57_000467"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HBE0890943.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTSAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(31981..32754)
                     /gene="srlR"
                     /locus_tag="KLK57_000468"
                     /gene_synonym="gutR"
     CDS             complement(31981..32754)
                     /gene="srlR"
                     /locus_tag="KLK57_000468"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HBE0890944.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFSFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            complement(32821..33180)
                     /gene="gutM"
                     /locus_tag="KLK57_000469"
     CDS             complement(32821..33180)
                     /gene="gutM"
                     /locus_tag="KLK57_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HBE0890945.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            complement(33286..34054)
                     /gene="srlD"
                     /locus_tag="KLK57_000470"
                     /pseudo
     CDS             complement(33286..34054)
                     /gene="srlD"
                     /locus_tag="KLK57_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311588.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
     gene            complement(34058..34429)
                     /gene="srlB"
                     /locus_tag="KLK57_000471"
                     /gene_synonym="gutB"
     CDS             complement(34058..34429)
                     /gene="srlB"
                     /locus_tag="KLK57_000471"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HBE0890946.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFTLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            complement(34440..35399)
                     /gene="srlE"
                     /locus_tag="KLK57_000472"
                     /gene_synonym="gutE"
     CDS             complement(34440..35399)
                     /gene="srlE"
                     /locus_tag="KLK57_000472"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HBE0890947.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSSPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSHFLTGAPTVLIAWFVSGFIYQ"
     gene            complement(35396..35959)
                     /gene="srlA"
                     /locus_tag="KLK57_000473"
                     /gene_synonym="gutA"
     CDS             complement(35396..35959)
                     /gene="srlA"
                     /locus_tag="KLK57_000473"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HBE0890948.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            36215..37300
                     /gene="mltB"
                     /locus_tag="KLK57_000474"
     CDS             36215..37300
                     /gene="mltB"
                     /locus_tag="KLK57_000474"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HBE0890949.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDRMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTRYSISQLATAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            37445..37942
                     /gene="pncC"
                     /locus_tag="KLK57_000475"
     CDS             37445..37942
                     /gene="pncC"
                     /locus_tag="KLK57_000475"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HBE0890950.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            38022..39083
                     /gene="recA"
                     /locus_tag="KLK57_000476"
     CDS             38022..39083
                     /gene="recA"
                     /locus_tag="KLK57_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HBE0890951.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            39311..39811
                     /gene="recX"
                     /locus_tag="KLK57_000477"
     CDS             39311..39811
                     /gene="recX"
                     /locus_tag="KLK57_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HBE0890952.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALVRDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WLNFAD"
     gene            39939..42569
                     /gene="alaS"
                     /locus_tag="KLK57_000478"
     CDS             39939..42569
                     /gene="alaS"
                     /locus_tag="KLK57_000478"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HBE0890953.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            42804..42989
                     /gene="csrA"
                     /locus_tag="KLK57_000479"
     CDS             42804..42989
                     /gene="csrA"
                     /locus_tag="KLK57_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HBE0890954.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            43305..43397
                     /locus_tag="KLK57_000480"
     tRNA            43305..43397
                     /locus_tag="KLK57_000480"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43339..43341,aa:Ser,seq:gct)
     gene            43401..43477
                     /locus_tag="KLK57_000481"
     tRNA            43401..43477
                     /locus_tag="KLK57_000481"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43435..43437,aa:Arg,seq:acg)
     gene            43541..43617
                     /locus_tag="KLK57_000482"
     tRNA            43541..43617
                     /locus_tag="KLK57_000482"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43575..43577,aa:Arg,seq:acg)
     gene            43681..43757
                     /locus_tag="KLK57_000483"
     tRNA            43681..43757
                     /locus_tag="KLK57_000483"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43715..43717,aa:Arg,seq:acg)
     gene            43956..44032
                     /locus_tag="KLK57_000484"
     tRNA            43956..44032
                     /locus_tag="KLK57_000484"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43990..43992,aa:Arg,seq:acg)
     gene            44313..44879
                     /gene="yqaB"
                     /locus_tag="KLK57_000485"
     CDS             44313..44879
                     /gene="yqaB"
                     /locus_tag="KLK57_000485"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HBE0890955.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVDVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRHYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            44876..45304
                     /locus_tag="KLK57_000486"
     CDS             44876..45304
                     /locus_tag="KLK57_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0890956.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKAIGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            45377..46933
                     /gene="gshA"
                     /locus_tag="KLK57_000487"
     CDS             45377..46933
                     /gene="gshA"
                     /locus_tag="KLK57_000487"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016159592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBE0890957.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGDAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHT"
     gene            47083..47598
                     /gene="luxS"
                     /locus_tag="KLK57_000488"
     CDS             47083..47598
                     /gene="luxS"
                     /locus_tag="KLK57_000488"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HBE0890958.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(47662..49200)
                     /gene="emrB"
                     /locus_tag="KLK57_000489"
     CDS             complement(47662..49200)
                     /gene="emrB"
                     /locus_tag="KLK57_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HBE0890959.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTKRRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(49217..50389)
                     /gene="emrA"
                     /locus_tag="KLK57_000490"
     CDS             complement(49217..50389)
                     /gene="emrA"
                     /locus_tag="KLK57_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HBE0890960.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            complement(50516..51046)
                     /gene="emrR"
                     /locus_tag="KLK57_000491"
     CDS             complement(50516..51046)
                     /gene="emrR"
                     /locus_tag="KLK57_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HBE0890961.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            complement(51137..51472)
                     /gene="ygaH"
                     /locus_tag="KLK57_000492"
     CDS             complement(51137..51472)
                     /gene="ygaH"
                     /locus_tag="KLK57_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HBE0890962.1"
                     /translation="MSYEVLLPGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            complement(51462..52199)
                     /gene="ygaZ"
                     /locus_tag="KLK57_000493"
     CDS             complement(51462..52199)
                     /gene="ygaZ"
                     /locus_tag="KLK57_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HBE0890963.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            complement(52323..53507)
                     /locus_tag="KLK57_000494"
     CDS             complement(52323..53507)
                     /locus_tag="KLK57_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0890964.1"
                     /translation="MTKPNHELSPALIVLMSIATGQAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            complement(53798..54790)
                     /gene="proX"
                     /locus_tag="KLK57_000495"
     CDS             complement(53798..54790)
                     /gene="proX"
                     /locus_tag="KLK57_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HBE0890965.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYCEGIFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWAEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            complement(54848..55912)
                     /gene="proW"
                     /locus_tag="KLK57_000496"
     CDS             complement(54848..55912)
                     /gene="proW"
                     /locus_tag="KLK57_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HBE0890966.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNSFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            complement(55905..57107)
                     /gene="proV"
                     /locus_tag="KLK57_000497"
     CDS             complement(55905..57107)
                     /gene="proV"
                     /locus_tag="KLK57_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HBE0890967.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAKLRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            complement(57463..58422)
                     /gene="nrdF"
                     /locus_tag="KLK57_000498"
     CDS             complement(57463..58422)
                     /gene="nrdF"
                     /locus_tag="KLK57_000498"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HBE0890968.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            complement(58432..60576)
                     /gene="nrdE"
                     /locus_tag="KLK57_000499"
     CDS             complement(58432..60576)
                     /gene="nrdE"
                     /locus_tag="KLK57_000499"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HBE0890969.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDATLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPVAGRINMSNLCSEIL
                     QVNCASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYAITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            complement(60549..60959)
                     /gene="nrdI"
                     /locus_tag="KLK57_000500"
                     /pseudo
     CDS             complement(60549..60959)
                     /gene="nrdI"
                     /locus_tag="KLK57_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
     gene            complement(60956..61201)
                     /gene="nrdH"
                     /locus_tag="KLK57_000501"
     CDS             complement(60956..61201)
                     /gene="nrdH"
                     /locus_tag="KLK57_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HBE0890970.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            complement(61449..61778)
                     /locus_tag="KLK57_000502"
     CDS             complement(61449..61778)
                     /locus_tag="KLK57_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBE0890971.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            61930..62274
                     /locus_tag="KLK57_000503"
     CDS             61930..62274
                     /locus_tag="KLK57_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HBE0890972.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            complement(62311..62760)
                     /gene="alaE"
                     /locus_tag="KLK57_000504"
     CDS             complement(62311..62760)
                     /gene="alaE"
                     /locus_tag="KLK57_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HBE0890973.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSHYQQVKA"
BASE COUNT        14861 a        16280 c        17009 g        14986 t
ORIGIN      
        1 atggcatatt ttatgttgtc attaatgatt tcaataatat gtggaaggtg atggaatttc
       61 agcaacagat ctattgcctc ttgttgctct gaatcattta aatcgaaaaa ccagtcacca
      121 ccgctggcaa gccacatatt tccatcgcca gtttcgaatg catccaacgc catcgcttcg
      181 tggttgcctt taaccgacgt aaaccagggt tggtttagca ggcgcagcac gttaagactc
      241 ttcggcccac gatcaatatt atcgcctgtg gaaataagta agtcggtttc agggtaaaaa
      301 gagagttgat gttaacggga ttgtaataac tgatattcac catgaatatc accaacgacc
      361 catatatggc gatagtgatg ggcattgatt ttttgatagc gtgtagatgg catggtttta
      421 ccctgtaaaa taagctttcc tattatacag ggtattttta tttgattcgt cagttgtcgt
      481 taatattccc gatagcaaaa gactatcggg aattgttatt acaccagact cttcaagcga
      541 taaatccact ccagcgcctg acgcggagtc agtgaatccg ggtcgagatt ttccagtgct
      601 tcgaccgcag gcgaagtttc ttctggcact gacagcaaag acatctgcgt accatccact
      661 tgcgtcgcgg cggcgttcgg cgaaatgctt tccagctcac gcagtttttg ccgtgcgcgc
      721 ttaataacct ctttcggtac gcccgccaga gcagcaaccg ccagaccgta gcttttgctc
      781 gccgcgccgt cctgcacgct gtgcataaag gcgatagtgt cgccgtgctc cagcgcatcg
      841 agatgtacgt tggcgacgcc ttccattttc tccggtagct gggtcagctc gaaatagtgg
      901 gtggcaaaca gcgtcaatgc cttaatctta ttcgccagat tttctgcgca cgcccacgcc
      961 agcgacagac catcgtaggt ggacgttcca cgcccgatct catccattaa caccagactg
     1021 tattcggtgg cgttatgtaa aatattggcg gtttcagtca tctccaccat aaaggttgag
     1081 cgcccggaag ccaggtcatc tgccgcgcct acgcgggtaa agatgcgatc gataggtcca
     1141 atctcgactt tttgtgccgg tacatagctg ccgatgtagg ccatcagcgc aatcagtgcg
     1201 gtctggcgca tataggtact tttaccgccc atgttcggac cggtgatgat caacatacgg
     1261 cgctgcggcg acagattcag cgggttagcg ataaatggct cattcagtac ttgttcaact
     1321 accggatggc gaccttcggt aatgcgaatg cccggtttat caatgaaggt cgggcaggtg
     1381 tagttcaggg tataggcccg ttccgccagg ttcaccagca cgtcgagttc cgccagcgcg
     1441 ctcgcgctct gttgcaacgc ttccagatgc ggcaacagca ggtcgaacaa ctcttcataa
     1501 agctgttttt ccagtgccag tgctttgcct tttgaggtga gaactttatc ttcgtactct
     1561 tttagctctg gaatgatgta gcgctcggcg tttttcagcg tctggcgacg catgtagttg
     1621 atgggtgcca gatggctttg cccacggctg atttgaatgt agtagccgtg caccgcatta
     1681 aagccaactt tcagcgtgtc caggccggta cgttcacgct cgcggacttc cagacgctcc
     1741 agataatcgg tcgcgccgtc agccagcgcg cgccactcat ccagctcttc gttatagccc
     1801 gatgcgataa caccaccgtc gcgtaccagc accggcggtg tgtcgatgat tgctcgctcc
     1861 agcagatcgc gcagctcggc aaactcgccc atcttctcac gtagcgcctg taccggtgca
     1921 ctatcgacag tttctaactg cgcacgcagc tccggcagtt gctggaaagc gtggcgcata
     1981 cgggccagat cgcgtgggcg agcagttcgt aaagccagac gtgccagaat acgttccagg
     2041 tcgccgacct gacgcagtac cggctgcaac tcggcggtga aatcctgcaa tgcgccaata
     2101 gtttgctggc gctcaagcaa cacgcgggta tcgcgcactg gcatatgcag ccagcgtttc
     2161 agcatacgac tacccatcgg cgtgacggtg cagtcgagca cagaagccag cgtattttcc
     2221 gcaccgcccg ccaggttctg agtaatttcc aggttacgac gtgtcgcggc atccataatg
     2281 atgctgtcct gctcacgttc catggtgata gaacgaatat gcggcagggt cgtgcgttgg
     2341 gtatctttcg catactgcaa cagacaaccg gcagcacaaa gtccgcgcgg cgcgttctcg
     2401 acgccaaaac cgaccagatc gcgggtgcca aattgcagat tcaactgctg gcgcgcggtg
     2461 tcgatttcaa actcccacag cgggcgacgg cgcaggccgc gacggccttc aatcagcgac
     2521 atctcggcga aatcttctgc atacagcagt tccgccggat tagtgcgttg cagctctgcc
     2581 gccatcgttt cgcggtcggc cggttcgctc aggcgaaaac gcccggagct gatatccagc
     2641 gtcgcgtagc cgaaaccttt gctgtcctgc cagatagccg ccagcaggtt gtcctgacgc
     2701 tcctgtaaca gggcttcatc gctgatggtg cctggcgtaa cgatacgcac aactttgcgc
     2761 tcaaccggac ctttgctggt cgccggatcg ccaatttgtt cgcagatggc aacggactct
     2821 ccctgattca ccagtttggc gagatagttt tccaccgcat ggtagggaat ccccgccatc
     2881 gggatcggct ctcccgccga agcaccgcgt ttggtcagtg aaatatccag cagttgcgac
     2941 gcgcgttttg cgtcgtcata aaacagttca taaaaatcac ccatccggta aaacagcagg
     3001 atctcgggat gctgggcttt cagcctgaga tactgctgca tcatgggcgt atgggcgtcg
     3061 aaattttcta ttgcactcat ggggttatgt ccggttccct gatattaaat ggggtgtgat
     3121 ggtttttatt ttcgttgtaa tcacataagg cgcacatgat accggagtta atcatttcag
     3181 gggaagaagc gaacggcgag ttcttgatgc tggttccggg aaagatgctg ttttgcagct
     3241 tttgcgtgat gcttatgaag caagtacgca aaattgcgtc ggttgaataa acctcctcct
     3301 tcagagccat agtccccttt caaattaagt tgtaatataa atacacctta agagtatgga
     3361 ggcagcatgt ccggcaagcg tatctctcgt gaaaaactga cgattaaaaa aatgatcgat
     3421 ctttatcaag cgaaatgccc gcaggcgtca gcggagccgg agcattacga ggcgttgttt
     3481 gtttacgcgc aaaagcggct ggataaatgt gtgttcggcg aagagaaacc agcctgtaag
     3541 cagtgtccgg tgcactgtta ccagcctgca aagcgtgaag agatgaaaca gattatgcgt
     3601 tgggccgggc cgagaatgct gtggcgtcat ccgatcttaa ccgtgcgtca tctcatcgat
     3661 gacaagcgtc cggtaccaga actgccggaa aaatatcgtc cgaaaaaacc tcgtgaatag
     3721 tatgccggac aggcagatct gtccggcaat ttgcagttaa atcaatgccg atttatcaat
     3781 tcccagccgc ttcatccgtg acagcagggt cgtgcgtttc agccccagac gttgcgcagc
     3841 gcctttaggc ccggcaacca cgccgttggt ttctttcagc acgcgcacaa tcaactgata
     3901 ttcatcttcg ccctcctggg cgacaaccgt tgcggcaggc ggcgtttcag gttccggtaa
     3961 aacaatatct ggcaatgaca gctgcagcac gttaccgcgt gttagcaata ccgcgcgctc
     4021 aatgacgttt tccagttcgc gtacgttacc cggccactcc atgttgctca aggtgcgcag
     4081 cgtctcggca ggaatgctgt cgatattgcg ccccagacga cgggcaattt tgaaggtaaa
     4141 ggctttcgcc agcagcggaa tatcttccgg acgctcgcgt agtggcggca ggtgaatcgg
     4201 gaatacgttc aggcggtaat agagatcgct acggaactct cggtcggcga ccattttttt
     4261 cagatcgcgg ttagtcgcgg caattaaacg tacgtccgtc tgaatgattt tgttgctgcc
     4321 gagacgttca aactcctgtt cctgcaatac acgcagcaac ttcggctgta actccagtgg
     4381 catatcgccc acttcgtcga ggaacagaga gcttttatcc gccagttcaa aacgaccaat
     4441 gcgctgggcg ctggcaccgg taaaagcccc acgctcatga ccaaacagat cgctttccag
     4501 caatccggca ggcatcgccg cgcagttcat tttgaccatg cggcgattat tacgcccact
     4561 gagattatgg atcgcgcggg caatcagctc tttacccgtg ccagtttcac cgaggatcag
     4621 cacggtactg tcactttgcg ccaccatttc aacttgttta agcacgctgt acatggcttc
     4681 gctgcggcca ataatctcgc caaattcact atcaacattg ttgagctgct cggtcagggc
     4741 gaggttttca tcaaccagcc gttctttcag acgatggatt tcctgatagg cgagggcgtt
     4801 atcgacagcg attgccacac gttcggcaat ctggcgcagc aaattcagat tggtagtggt
     4861 aaacactttc tcttcgcatt gcgccagttt cagcacgccc agcatggtgt cgccagacat
     4921 cagcggtaac aggcacaagg tttgaatctg gttgccctag gtgtcgaaca acatgcgttc
     4981 atagggggct aaatcgtccc gctcgtggag attgatcagc agcatctctt tacttttgaa
     5041 cacgcgttcg gtgagggttc cggcttcatc gacttcgctc tgttcgtggg cgggatgttg
     5101 tttatcaaga tagtgagtgg agtagatgtt gagtttgttt ttacggtggc tgcgtaagac
     5161 gatactgata tcgtcaatgt cgaaatagta atggatttct ttggcgactt cgctgaccag
     5221 ttcgtccata tccaggcggg aaagcaccgc gttggtaatg gcaaccagga tgcggaagtt
     5281 atcgcgctcc cggcataaca actcatagtc gacattgttg acgacgcggc tctggatttg
     5341 ttcggtgacg acagaaacga tctgcgtaaa tgtttgcaga cgattgaact ctttttcgct
     5401 ccagggacga tcgtcataac gaataaattc acagccacca aaaatatgcc cttccgccgc
     5461 cagtggcatc aggcaatagt gaccaaattt ggggtatagc ccacctgcga ccagctgcgg
     5521 ccaggtttca caaaattctt cgtaactgca atgcagcgta tcagggcgcg acaaaatgcg
     5581 gcgtaccgga ccgtgtgcca gaacagtttc gtcttcatat ttaatggggt gtctttttca
     5641 cgcgatgcgt aataagacgc acgttgagtc tgcgcttgcc acaacacaat agccgcgttg
     5701 tcggcgagcg cagaacgctt taccagttgc gaaagagcct cacacagcga ggccagatcg
     5761 ggctgctgca atagtgtccg agtgatgtcg aacaaccctt gctgtccgag atcactcatc
     5821 ggtgtatatg acattttgtt cttccaggaa agcaccgcaa agcggtgcaa aaggtcattg
     5881 actaactata ggagatttag aattttatta gcatatacgc ggaagaggtt cggcgtgtgg
     5941 taaatcgagg gttcgtttca caccatacag accggcaaga cgaacacctt tacgttccac
     6001 cacttcacca attagcgccg cgtctttccc cagtggatgg gaatgtaacg ctgccagcac
     6061 ttgctctgcc gcgttgcgtt caacggcgat caccagtttg ccttcgttgg caaagttaag
     6121 ggcgtccagt cccagcagtt cgcaaacgcc gcgcacggca ggtttaaccg gcagtgccac
     6181 ttctgaaatt tcaataccac aaccgcaggc tgccgcgaac tcatgaacta ccgcgttcac
     6241 accaccacgg gtggcatcac gcagcgcttt cacgccggga atgtcacgca gcgtctgaat
     6301 aagcggcgtc agtaccgcgc agtcgctgac cagttcgcca tccagcccca gctgctcacg
     6361 caggttaagg atagtcgccc cgtggtcccc gagcgtgccg ctaaccagta atacatcgcc
     6421 tgcggttagc gtctgtgcgc cccagtgaat attcgccgga attgcgccca tgccagcggt
     6481 gttgataaac agtttatctg ccgcgccgcg ctgcaccact ttagtatcgc cagtaacgat
     6541 ggcaatgcct gccgcgcgcg cggtttctgc catgctggtc actacggctt tcagtgtctc
     6601 catcggcaat ccttcttcga ggataaagcc acaggagaga tagcgcggaa tagcgccact
     6661 gaccgcaacg tcattggctg tgccgcaaat cgccagcttg ccgatattac cgccaggaaa
     6721 gaacagcggg tcaatgacgt aactgtcggt ggagaacgcc agacggtcgc cttctgctac
     6781 cagctgcgcc agatcaagac gtgcctggtc ttcctgctct gccagccacg ggttggcaaa
     6841 ggcttccata aacaggctgt tgattaattg ctgcatcgcc tggccgccgc tgccgtgggc
     6901 gagttggata ttattcacgc ttcattctcc tgctgacgat actgatacca cgcggcgcac
     6961 gctccttcgg aggagaccat cagtgcacca aatgcggttt gaggattaca ggtgttacca
     7021 aacagcgggc attgatgcgg cttacatttg cccgttaata cctcaccaca acgcgcgcgc
     7081 gggtcatcgc agacctgctg cggtgctggg cggaaatgtg cttcggcatc gaatcgttga
     7141 taatccggcg tcaggtgcac gccagaagat tcaatcacgc ctaagccgcg ccattcgctg
     7201 tcgccgttga cacagaacac atcagcaatt gcctgttgcg ccagcaggtt accggcatcc
     7261 ggtaccaccc gacgatactg attctctacc ttgctgtggg ccgctatttt ctgctccacc
     7321 agcatgacca cgccttgcag tagatcaagg ggttcgaaac cagcgaccac cagcggacga
     7381 tgaaaatcgc tggcgataaa attataggca tcagtgccga taaccatact aacgtggccc
     7441 ggcgcgagga acgcgtcgat accgttatcc ggctgttcca gcaaactgcg cagcgtcggg
     7501 ataagcgtaa tatgctggca gaagaagtaa aaattctgca catcgcgggc tttcgcctgt
     7561 tgcagggtga tggcggtggt cggcatggtg gtttcaaaac ctaagccgaa gaatacgact
     7621 ttacgggttg gattctcctg cgccagtttc aacgcatcca tcggcgagta aacgatgcgc
     7681 acatcggcac cgcgtgcttt tgcctgcaac agcgatccct gtttccccgg cacgcgcatg
     7741 gcgtcgccaa aggtacagaa gatgacttcc ggatggctgg caatctccac gcaggtgtcg
     7801 attctaccca tcggcagtac gcacaccggg caacccggac cgtggataaa ctcaacgttt
     7861 tcaggcagta actggtcgag gccgaattta aaaatggcgt gggtatgacc accgcacact
     7921 tccataatcc gcagagggcg ttcggcggtg taagagagat gtgaagcacg ttcgcgcaga
     7981 tgctcaatta actgcatcac ctgttccggc gcgcgatatt catcaacaaa acgcattatt
     8041 tttcctcgcc atacaacagc gcgccgacat ccggctcaac gtcaaacatg ttttgtaagg
     8101 cgtcgagagt gtcgcgtgct tcggcttcat taattacgct catggcaaag ccaacgtgta
     8161 ccagtaccca ctggcccacg cgcggctgac cgttttcatc gcagctgccg actaacgtta
     8221 aatcgacatc gcgctgaatg ccgcagacgt cgacttttgc ctggttgccg tcaatggtgc
     8281 ggatctggcc gggaacgcct atgcacatcg ctgtgtctcc agccagttca gccactggtc
     8341 catcccttcg ccgctggtgg cggaaataag gatgatttca atttctggat tgacttcgcg
     8401 ggcgcaggcg atgcacttct caacgtcaaa gttgagatac ggcaacaggt caactttgtt
     8461 gagcagcatc agcgaggcgg cggcaaacat atgcggatat ttcagcggtt tgtcttcacc
     8521 ttcggtaacg gaaagcaccg ccactttgtg tttttcaccg agatcgaagc tggccgggca
     8581 tacgaggttg ccaacgtttt cgataaacag aataccgtta tcgtccagtg gcagacgcgg
     8641 tgcggcgtcg gcaatcatct gtgcgtcaag atggcagcct ttaccggtgt tcacctgaat
     8701 cgctggtgtg ccggtagcgc gaatgcgtgc ggcatcgttc acggtttgct ggtcaccttc
     8761 aataaccgcg cacggaacgc tgtctttcag gcgcattagg gtttccgtca gcagggtggt
     8821 tttaccggaa ccagggctgg aaaccaggtt gagcaccagt tgcttgcggg cagcaaagcg
     8881 cgcgcggttg cgttcagcca gacggttatt tttgtccagc acgtcaattt cgacttccag
     8941 catccgacgc tggctcatgc ccggcgcgtg agtgcccgct tcgccatgac cgtaatgcag
     9001 gtcgccttct tcagtctggc tgggggtaaa ttcaggcgct ttaatgccgg tgattttcat
     9061 cttcgggcgt gccgccgggg caaatggcgc gctacgaaac gcggaatgag ggttatgttc
     9121 atcaccctcg atatacaggt tgccttcacc gcaaccgcat gttgtacaca tcgctcactc
     9181 ctggtctatt tctatccgcc gaatctgtaa accgtcgtct gccacaatct gtagcatgtc
     9241 accatgacac tgtggacagc ggcggacgcg ctgggtcagt agcgtcacat actgttggca
     9301 tgtttcacac cagcattcgg cctcttgttc ttcgaggtgc agtttacaac cttccgccac
     9361 gctgccgcgg caaaccagat caaaacaaaa ggcaagagag ctggtttcga cacaagaaaa
     9421 tgcgccaatt ttgagccaga ccccagttac gcgttttgcg ccgtgttttg cggcctgctg
     9481 ttcgatcaat tccagtgccc gttggcagag ggttatttcg tgcatatcgc ctcccattaa
     9541 ctattgccag ctacaagcaa taattgtgcc agtgttgatt atccctgcgg tgaataatgt
     9601 cgatgatgtc gaaatgacac gtcgacacgg cgacgaaatt catctttagc ttaaaaatct
     9661 atttaataac aataaattaa aagttggcat agaaaatgct taaagctggc atctctgtta
     9721 aacgggtaac ctgacaatga ctatttggga aataagcgag aaagccgatt acatcgcaca
     9781 gcggcattgt cgcctacagg accagtggca catctactgc aattcgctgg ttcaggggat
     9841 cacgttatcg aaagcgcgcc tgcatcacgc catgagctgc gcgccggaca aagaactctg
     9901 tttcgtcctt tttgaacatt ttcgtattta cgtcaccctg gcggatggct ttaacagcca
     9961 caccatcgag tattacgtcg aaacaaaaga tggcgaagac aaacagcgga ttgcgcaggc
    10021 gcaactgagc attgacggca tgattgatgg caaggtcaac atccgcgatc gcgaacaggt
    10081 tctggaacac tatctcgaaa aaatcgctgg cgtttacgac agcttataca ccgctattga
    10141 aaacaatgtg ccggtgaatt taagccaact ggtaaaggga caaagcccgg cagcatgagc
    10201 tgaggctttg cccgttttgc aggcgttacg cctgtttggg gatgggcgtg tcgatgagtg
    10261 tcgaaaatga catttcatcg gcatgttttc gtcaaaaatg acaatcacct gaggaatgcc
    10321 tggtgaatcg ttttgtaatt gttgactcca cgctctgtat cggctgccac acttgtgagg
    10381 ccgcctgttc agagacgcat cgccagcacg gcctgcaatc aatgccgcgc ctgagagtga
    10441 tgctgaatga aaaagaatct gcgccgcagc tctgtcacca ctgtgaagat gcaccctgtg
    10501 cggtggtttg cccggttaac gccatcaccc gcattgatgg ggccgtgcag ttgaatgaaa
    10561 gtctgtgcgt aagctgcaaa ctgtgcggca tcgcctgccc gtttggcgca attgaatttt
    10621 ccggcagccg tccgctggat attccggcaa acgccaatac cccgaaagcg ccaccggcac
    10681 cgcctgctcc ggcgcgtatc agtacgctgc ttgactgggt gccgggtatt cgcgcgatcg
    10741 ccgtcaaatg tgacctttgc agttttgatg aacaaggtcc ggcctgcgtg cggatgtgcc
    10801 cgactaaagc cctgcatctg gtggataaca ccgatatcgc ccgcgtcagc aaacgtaagc
    10861 gtgagctgac ctttaacacg gactttggcg atctcacctt gtttcagcag gctcaaagtg
    10921 gagaggctaa atgagcgcaa tttccctgat caatagcggc gtggcatggt ttgtcgccgc
    10981 cgctgttctg gcatttctct tttcttttca aaaagcgtta agtggctgga tagctggaat
    11041 tggcggcgcg gttggtagtc tgtatacggc agccgcgggc ttcactgtac tgactggcgc
    11101 ggttggcgtg agcggtgcgc tgtcgctggt aagctacgat gtgcaaatct ctccgcttaa
    11161 cgcgatttgg ctgattacgc tcggtctgtg cggtctgttt gtcagcctct acaacattga
    11221 ctggcatcgc cacgcgcagg tgaagtgcaa cggcttgcag atcaatatgt tgatggctgc
    11281 cgccgtctgc gccgtcatcg ccagcaacct cggcatgttc gtggtaatgg ccgaagtcat
    11341 ggccttgtgt gcggtgttcc tcaccagcaa cagcaaagag ggcaaactgt ggtttgcgct
    11401 ggggcgtctt ggcactctgc tgctggtgat tgcttgctgg ctgctgtggc agcgttatgg
    11461 cacgctggat ctgtgcctac tggatatgcg tatgcaacag ctgccgctcg gttccgatat
    11521 ctggctgctc ggtgtgattg gctttggcct gctggccggg attattccgc tgcacggctg
    11581 ggtgccgcag gcacatgcta acgcctctgc gccagctgcc gcgttgtttt ctacggtagt
    11641 catgaaaatt ggcctgctgg gcatgttaac cctgtcactg ctgggcggta atgcaccgct
    11701 gtggtggggg atcgcgctgc tggtgctcgg catgatcacc gcgttcgtcg gtggtctgta
    11761 tgcgttgatg gaacacaaca tccagcgcct gctggcttac cacaccctgg aaaatatcgg
    11821 cattatcctg ctggggctgg gcgctggcgt aacgggtatc gcgctcgaac aaccggcgct
    11881 gattgctctt ggcctggtcg gtggtctgta ccatctgctt aaccatagcc tgttcaaaag
    11941 cgtactgttc ctcggggcgg ggagcgtctg gttccgtacc ggtcatcgcg atatcgaaaa
    12001 actcggtggt attggcaaga aaatgccggt tatctccatc gccatgttag tcgggttgat
    12061 ggcaatggct gcgctgccgc cactgaacgg gtttgccggg gaatgggtta tctatcaatc
    12121 cttcttcaaa ctgagcaata gcggcgcgtt tgttgcccgt ctgctggggc cgctgctcgc
    12181 tgtgggtctg gcaattaccg gtgcgctggc ggtgatgtgt atggcgaaag tctatggcgt
    12241 cacgttcctc ggcgcgccgc gtaccaaaga agccgaaaac gccacctgtg cgccgctcct
    12301 gatgagcgta agcgtagtgg cactggcgat ttgctgcgta attggcggtg ttgctgcgcc
    12361 gtggctgctg ccgatgctct ctgctgctgt acctctgcct ctggagcctg ctaacaccac
    12421 cgtttctcaa ccaatgatca cattgctgct gattgcctgc ccgctgctgc cattcatcat
    12481 tatggcgatt tgcaaaggcg accgcctgcc gtcacgttcc cgcggtgcgg cctgggtgtg
    12541 cggctacgac catgaaaaat caatggtgat taccgctcac ggttttgcca tgccggttaa
    12601 acaggcgttt gcgccagtgc tgaaactgcg caaatggctg aatccggtgt cgctggtgcc
    12661 gggctggcag tgcgaaggta gtgcgttgct gttccgccgg atggcgctgg ttgaactggc
    12721 ggtactggtg gtgattattg tttcacgagg agcctgagaa tgagtgtttt atatccgtta
    12781 attcaggcgc tggtgttatt tgccgttgcg ccgctgctct ccggtattac ccgcgtggcg
    12841 cgcgcccgtc tgcataaccg tcgtgggccg ggtgtgttgc aggagtatcg tgacattttc
    12901 aaactgctgg gccgccagag cgttggcccg gatgcctccg gctgggtgtt ccgcctgacg
    12961 ccgtatgtga tggtgggcgt catgctgact atcgctactg cgctgccggt ggtgaccgtc
    13021 ggttctccgc tgccgcaact gggtgattta atcaccttac tgtatctctt tgccatcgcg
    13081 cgtttcttct ttgccatttc tggtctggat accggtagcc cgtttaccgc tatcggcgcg
    13141 agccgtgaag cgatgcttgg cgtgctggtc gaaccgatgc tgctgcttgg tctgtgggtt
    13201 gccgcacagg ttgccggttc caccaacatc agcaacatca ccgacaccgt ttatcactgg
    13261 ccgctgagcc agagcatccc gctggtactg gcgctttgtg cctgtgcgtt cgccaccttt
    13321 atcgaaatgg gcaaactgcc gttcgacctg gcggaagccg agcaggagct gcaggaaggc
    13381 ccgctctctg aatacagcgg cagcggcttt ggcgtcatga aatggggtat cagcctgaaa
    13441 cagctggtgg tgctgcagat gttcgtcggc gtatttatcc cgtggggaca gatggaaacc
    13501 ttcaccgccg gtgggctgct gctggcgctg gtgattgcca tcgtcaaact ggtcgtcggc
    13561 gtcctggtta tcgcgctgtt cgaaaacagc atggcccgtc tgcgtcttga tattactccg
    13621 cgcattacct ggactgggtt tggctttgca tttttagcgt tcgtctcctt gctggcggcg
    13681 tgattaaaga gagtttgagc atgtctgaag aaaaattagg tcaacattat ctcgccgcgc
    13741 tgaatgaggc atttccgggc gtcgtgctgg accacgcctg gcagaccaaa gatcagctga
    13801 ctgtcaccgt aaaggtgaac tatctgccgg aagtggtgga gtttctctac tacaagcagg
    13861 ggggctggct gtcggtgctt tttggtaacg acgaacgcaa actgaatggt cattacgccg
    13921 tttattacgt gctgtcgatg gagaagggga ctaagtgctg ggtaacggtt cgcgtcgaag
    13981 ttgatgccaa caaaccggag tatccgtccg tgacgccgcg cgttccggcg gcggtgtggg
    14041 gcgagcgcga agtgcgcgat atgtacggtt tgattccggt tggtctgccg gatgaacgcc
    14101 gtctggtgct gccggatgaa ctttatccgc tgcgtaaaga cagcatggat tatcgtcagc
    14161 gtccggcacc gaccaccgat gctgaaacct acgagttcat caacgaactg ggcgacaaga
    14221 aaaacaacgt cgtgccgatt ggtccgctgc acgtcacttc tgacgaaccg ggccacttcc
    14281 gtctgttcgt cgatggcgaa aacattatcg acgccgacta ccgtctgttc tacgttcatc
    14341 gcggcatgga aaaactggcg gaaacccgta tgggttacaa cgaagtgacc ttcctctctg
    14401 accgtgtgtg cgggatctgc ggctttgccc acagcaccgc ctacaccacg tcggtagaaa
    14461 acgcgatggg tattcaggtg ccagaacgtg cgcagatgat ccgcgccatt ctgctggagg
    14521 tggaacgcct gcactcgcat ctgctcaacc tcggtctcgc ctgccacttt accggctttg
    14581 actccggctt tatgcagttc ttccgcgtgc gtgaaacctc catgaaaatg gcagagatcc
    14641 tcaccggtgc gcgtaaaacc tacggcctga acctgatcgg cgggattcgt cgcgatctgt
    14701 tgaaagatga catgatccag acccgccagc tggcgcaaca gatgcgtcgt gaagtgcagg
    14761 agctggtgga tgtgctgctg agtacgccga acatggaaca gcgcaccgtc ggcattggtc
    14821 gtctggaccc ggaaatggcc cgcgatttca gtaacgttgg cccgatggtt cgcgccagcg
    14881 gtcacgcccg cgatactcgc gccgatcacc cgtttgtcgg ttatggcctg ctgccaatgg
    14941 aagtccacag cgagcagggc tgcgacgtta tttcccgtct gaaagtgcgt atcaacgaag
    15001 tttataccgc gctgaacatg atcgactacg gtctggataa cctgccgggc ggtccgctga
    15061 tggtggaagg ctttacctac attccgcacc gtttcgcgct gggctttgcc gaagcgccgc
    15121 gcggcgacga tatccactgg agcatgaccg gcgacaacca gaagctgtac cgctggcgct
    15181 gccgtgccgc gacctacgcg aactggccga ccctgcgcta catgctgcgc ggtaacaccg
    15241 tttccgatgc gccgctgatt atcggtagct tggacccttg ctactcctgt accgaccgca
    15301 tgaccgtggt cgatgtgcgt aagaaaaaga gcaaagtggt gccgtacaaa gaactcgagc
    15361 gttacagcat tgagcgtaaa aactcgccgc tgaaataagg aatcgccatg tttaccttta
    15421 tcaaaaaagt catcaaaacc ggcacggcga cctcgtctta tccgctggag ccgattgcgg
    15481 ttgataaaaa cttccgtggt aagccggagc agaacccgca gcagtgcatc ggctgtgcgg
    15541 cctgcgtcaa tgcctgcccg tcaaacgcct taacggttga aactgacctc gccaccggtg
    15601 agctggcgtg ggagtttaat ctcgggcgct gcatcttctg tggacgctgc gaagaagttt
    15661 gcccgacggc ggcgatcaaa ctgtcgcaag agtacgaact ggcagtgtgg aagaaagaag
    15721 acttcctgca acagtcccgc ttcgcgctgt gcaactgccg cgtctgcaat cgtcctttcg
    15781 ccgtccagaa agagatcgac tacgccattg cgctgcttaa gcacaacggc gacagccgcg
    15841 cggaaaacca ccgcgaaagc tttgagactt gcccggaatg taagcgccag aaatgcctgg
    15901 tgccgtccga ccgtattgaa ctgactcgcc atatgaaaga ggccatctga tgagcaattt
    15961 attaggcccc cgtgacgcca acggcattcc ggtcccgatg acggtggatg aatccatcgc
    16021 cagcatgaag gcgtcgttac tgaaaaaaat caaacgttct gcctacgttt atcgcgtgga
    16081 ctgcggcggc tgcaacggct gcgaaatcga aattttcgcc acgctttcgc cgctgtttga
    16141 tgcagaacgc ttcggcatta aagtcgttcc ttcaccgcgt catgcggata ttttactgtt
    16201 taccggcgcg gtcacccgtg caatgcgatc ccctgcgctg cgtgcgtggc agtccgcgcc
    16261 ggacccgaaa atttgtatct cctacggtgc ctgcggtaac agcggtggta ttttccacga
    16321 tctctactgc gtgtggggcg gtacggataa aatcgtcccg gtagatgtct acattcctgg
    16381 ttgcccgcca acgcctgccg ccacgctgta cggctttgca atggcgctcg gcctgctgga
    16441 gcagaaaatt cacgcccgtg gcccgggtga gcaggatgaa caacaggcgg aaatcctgca
    16501 tggcgatatg gtgcagccgc tgcgtgtgaa agtggatcgc gaagcacgcc gcctggcggg
    16561 ttatcgttac ggtcgtcaga ttgccgatga cttcctgaca cagttagggc agggcgaaga
    16621 acaggttgca cgctggctgg aagcggaaaa cgatccgcgt ctgaacgaga ttgtcagcca
    16681 tctgaatcat gttgttgaag aggcgcgtat ccgatgagtg aaaaggtggt gttcagtcaa
    16741 ctgagccgta aatttattga tgagaacgat gccacgcccg ccgaggcgca gcaggtggtc
    16801 tattacagcc tggcgattgg tcaccacctt ggggttatcg attgcctgga agcggcgctc
    16861 acctgcccgt gggatgaata tctggcatgg attgccactc tggaggcggg cagtgaagcc
    16921 cgccgcaaaa tggaaggcgt gccgaaatat ggtgagatcg tcatcgacat taaccatgtg
    16981 ccgatgctgg ccaacgcatt cgataaagcc cgggcagcgc aaacttcgca gcagaaggaa
    17041 tggagtacaa tgctgttaag tatgctgcat gatattcatc aggaaaacgc catctatttg
    17101 atggtgagga gactgcgtga ctgacgtttt actctgtgtt ggcaatagca tgatgggcga
    17161 tgatggcgct ggcccgctgc tggcggaaaa gtgcgccgcc gcgccgaaag gtaactgggt
    17221 ggtgattgat ggcggtagtg caccggaaaa cgacatcgtc gccatccgtg aactgcgccc
    17281 tacgcggtta ctgattgttg acgctactga tatggggcta aaccccggcg aaatccgcat
    17341 catcgacccg gatgatatcg ccgagatgtt tatgatgact acccataaca tgccgctgaa
    17401 ctatcttatc gaccagttga aagaagacat tggcgaagtg attttcctcg gcatccagcc
    17461 ggatatcgtc ggcttttact acccgatgac tcagccgatt aaagatgcgg tagaaaccgt
    17521 ttatcaacga ctggaaggct gggaagggaa tggcggcttc gcgcagttag cggtggaaga
    17581 agaataattc tccatcaggg aattcagcga aatgaatatc agaacaggga tgtgcgcttt
    17641 tttattgagc ttagtgctgc ctgcacaggc caccagcttt accgaatatc tgccaatgag
    17701 tgacagcgaa tacgcgcaaa agcgggcgct gaaacctctc ctgaccatgc cttatgatgc
    17761 cgagcaaacc tggcgtttta gaaaagtggg cgttgctggc gtgacgcttg aaaagatgcc
    17821 aaatgatgat agtgaatggc aattgaacgg taaagatcgc gccggtaaat cctggtctgt
    17881 gcctgtgggt atgctggcaa acatagcggg caaagggcaa ctctatcgcg ccgatcttga
    17941 tcgtaacggc attcaggatc tggttatctg gttaccaagt accggtaatg gtctggctcc
    18001 ttatgcacat ttaattctta tgacgttcac gcgcgaaggc cgtccctgtg tctttgaacc
    18061 caggggattc tacaccgcaa gcaaaactgg cgtagatgat ttgctggatt tgcaaggcaa
    18121 tggtcacacg caattgctgg atatgcagtt tgattccggt tactggataa ccagtctgta
    18181 tcaggtaaaa gacgccaaat ggcagcgtgt tcacggctgg tttggcaagt taagctatcc
    18241 cgcgctcacg cgttttactt atacccccaa ccgtaaactc gtcctcaaac ccatcgccgg
    18301 gcgcgacccg caaacggaag atctggcgca gacgcagcgt tgcttgataa agggggatgt
    18361 tcttgatggg gtgaaccaga actgactttt tgcagagtgg ctatgaggat tttgggggta
    18421 attatatcgc gctcaagtgt ttacatattg tgaacttgga tttgatgagc tatagtttcc
    18481 gaacacataa ggatagaact cgcagatgtg gctgtttact tactataagg aagttaaaac
    18541 ttaaaaatgc acatcatttc taaagcgcca tttgaagagt ctgcgcgtaa atatcctaac
    18601 gacgctctgg ctcttcaggc attgtatcgg gttattaagg aaacggattt ttctacgcca
    18661 gaagagatgc gaacggcgtt tcccaacctc gacaatttta aatatcgcaa caaatggtac
    18721 gttctggatg taggtgggaa caacctaagg gtgattgcct acataaactt tgttaataag
    18781 cggttctttg tgaagcacat aactaatcac gccgagtatg acaaactgac acgttactac
    18841 agagagaaca aagaatgact gctaatgctg cacgcgctgt taaagcaaca agagaactgg
    18901 tcaatgcagt cccctttctg ggaggtagtg actcagaaga tgattatcgt gaagcactgg
    18961 aactggtcga atatttgatt gaagaagatg acaccaatcc acttatcgac tttctggcga
    19021 gccgcattgc agaatatgaa aacaacaacc aagagtttgc agagttcgac aaagccgttg
    19081 cggcaatgcc tgttggcgtg gcgttacttc gcacgctgat tgatcaacat aatctgacct
    19141 atgcggattt gaagaacgaa atcggctcta aatccctggt cagtcagatt ttgtctggac
    19201 aacgatccct gacgatatcc cacattaagg cactttcagc ccgatttggc gttaagccgg
    19261 aatggtttct ttgattttga ctgatgccaa accccagatt gcccgatgcg ctgcgcttat
    19321 cgggccttcc tggttcgtgc gacatgtagg ccggataagg cgttcacgcc gcatccggca
    19381 ctgttaccta ctctaaatct tccccattac tggcaatcac ttttttatac caccagaacg
    19441 atttcttacg tgtgcgcgtc agcgtgccgt tgcctgcgtc gtcacggtct acgtagacaa
    19501 aaccgtagcg tttgctcatt tcacccgtag aggcggagac taaatcaata cagccccagg
    19561 tggtgtagcc catcagcgga atgccatctg caatcgcttc gcccattgcg cggatatgtt
    19621 cgcgtaaata gctgatgcga tagtcatcgt taatctcgcc attggcagca agttcatctt
    19681 ttgcgccgag gccgttttcc accagaaaca gcggcttctg ataacggtcg tacatcatgt
    19741 tcatggtgat acgcagaccg agtggatcaa taccccagcc ccagtcgctc acttgcagat
    19801 acgggttacg cagcgatttc actacgttcg ccgcactgct attgttggcg ttcatctccg
    19861 ccgaggcgca gcgcgaggcg taatagctga aagagacaaa atcgacggtg tttttcagga
    19921 tttcatcatc gcccggtgct ttgtcgatgg ttaccccttt ttcgcggaat acgcgggcag
    19981 agtaagccgg atacgtgccc cgcgcctgca catcgataaa aaacaggttt tcccggtctt
    20041 tctccagcgc cgcccagaca tcttccggct tgcaactgta agggtagaag ttaccgcccg
    20101 ccagcataca gcccacctga ttttgcgggt taacctcatg ggcgattttg gtggctagcg
    20161 cactggcaac cagctggtga tgcgcggcct gatatttcac ctgatcctga ttttcacctt
    20221 cttcaaacac cagacccgcg ccggagaacg ggctatgcaa cataatgttg atttcattga
    20281 aggtaagcca gtatttcacc agaccatcaa atgcttcaaa gcaggtccgg gcgtagcggc
    20341 tgaaaaactc caccagcttg cggttacgcc aggagccata ttcggtgacc agatgcatcg
    20401 gcacatcgaa gtggcacaac gtgaccagcg gttcgatacc gtacttttta cactcttcaa
    20461 agacagaacg ataaaaagca atgccctgct gattgggcgt gatttcatcg ccctgcggaa
    20521 agagacggct ccaggcaatt gaggtacgga aaaccttgaa tcccatctct gccatcaggg
    20581 cgatatcttc tttataacga tgataaaaat ccgtcgcctc atggctggga taaaactcgt
    20641 catcgcgcaa ctgaaaacgt ttttccagcc ccagtttcac cgccattcga tgctcgccgt
    20701 gtgggatcat atcgacagtg gtcagccctt tgccaccttc acggaacgca ccttcagact
    20761 ggttggcggc aagcgcgccg ccccataaaa aaccttctgg aaatactgac attttcatcc
    20821 tcaattaaga cttacttctt tggcgacggt cggttgtacg ctctgatgct ttcgcgcctc
    20881 ggcagccgct tcctcaacag gaatatcctc aaagccgagc aacagtgtga ggataaacga
    20941 cagcaccacc gccagcgcca tgaccgcgaa cacccagacg atgctcattg gattcgccgg
    21001 atcgaagaat tgcacgctgg taaataaccc tggcgctgcc attgagtggc tggcaagccc
    21061 cgccataccg gcaaccgcgc cgcaaataaa accgctgata agactggcga taagcggacg
    21121 tttcaggcgg atcgccacgc cgtataacgc cggttcggaa atccccgcca taatggctga
    21181 tgccgccgca gccagcgccg tctggcgcag ttccgggttt ttcgttttcc acgccaccgc
    21241 cagtgatgaa ccgcccagcg acaggttagc gccgatctct gacggcatga ccatcccttc
    21301 tttgccggtt tcggcaatgg tctgaatgat ggttggcgta aagacgcggt gcatcccggt
    21361 cattaccagc agaggccaca gcgcgcccat aatggcgact gaaagccagc ccagataacc
    21421 atgaatggtg taaaccagcg ccgaaatggc gctaccgatc cagataccaa tcgggccaat
    21481 cagcaggatt gccagcggtg cggcaatcaa cacaatcagc attggcttga ggaagttttt
    21541 ggtcaccgcc ggtgtaatgc gatccaccca gcgttcgata tatgacaggc accaggtcat
    21601 caccagcgcc gggatcactg tgtaggtgta tttcaccgcg gtgaccggaa tcagggcaaa
    21661 ttcgacatgt tcaccctggg ccgctttcgc catcagttca ataaaactcg gatgtaccag
    21721 cacacccgcg atggcaatcg ccagcgacat attggtttta aatttgatgg cggcagatgc
    21781 cgcgaccatc agcggcagga agaagaaagc accgtcacca atcacattca gaatggttaa
    21841 ggtcggcgat ccttttgtca gcacgccgct catctcgagg atcatcgcca gcagtttgac
    21901 catcgatccg ccgataatcg ccgggatcag cggtgacatg gtgccgatca gcgcatcgag
    21961 gatccccgca ccaatgcgac gcagcgtgag tttgggttta cccacgggct gtgcgggctg
    22021 catatctccc ggcagcaggc tgacgacttc ctgaaaggct tgtgaaacgg tattgccgat
    22081 aatcacctga cactggttgt cactacgtac cacgccgagc acgccgggga tggtttttaa
    22141 cgtcgggctg tcgataagct ggtcatcttt gataacaacg cgcaaacgcg tcatacagtg
    22201 cgtgaccgcc gagatgttat caacgccgcc cagtgccgct atcaccgagc gtgccagcgc
    22261 cgcataattt ttggccatcg gattttatcc tgttatcagt agggtagatg tagttgttat
    22321 cagtcgacgt aacggaaaaa tgccgatgct gaaacggaat taggaaaccg gtttcacaaa
    22381 atcatgaata gttgtcacta aataagcaag agagttaatt gttcattttt tatatttgtg
    22441 aaaccggtca cctgaatatt tttcccgccg attgcgcaag gacaatcggc aaacggtgca
    22501 gtacactgcg ttgattgcgt gtaaagggat gaaaatgatg acgacgatgc tggaagtggc
    22561 gaagcgcgcc ggggtttcaa aagcgaccgt ttcccgcgtg ctttcaggta atggctacgt
    22621 cagccaggag actaaagatc gcgtgtttca ggcggtagaa gagagcggtt accgcccaaa
    22681 cttgctggcg cgcaatctgt cggcgaagag tactcagacg ctggggttgg tagtgaccaa
    22741 cacgctttac cacggcattt attttagtga attactcttt catgccgcgc gaatggcgga
    22801 agagaaaggg cggcagttgc tattggcaga tggtaaacac agcgccgaag aagagcgcca
    22861 ggcgattcag tatctgctgg atttgcgctg cgacgccatt atgatttacc cgcgtttttt
    22921 aagcgtggat gagatcgatg acatcattga cgcgcacagt cagccgataa tggtgcttaa
    22981 tcgccgcctg cgcaaaaaca gcagccatag cgtctggtgc gatcataaac agaccagctt
    23041 taatgccgtg gcagagttga taaacgccgg acatcaggag attgctttcc ttaccggctc
    23101 gatggattcc cccaccagca ttgaacgtct tgctgggtat aaagacgcgc tggcgcagca
    23161 tggtattgcg ctcaatgaaa aacttatcgc taacggtaaa tggacgcccg ccagcggggc
    23221 cgaaggggta gaaacgttgc tcgaacgtgg ggctaaattt agcgcgttag ttgccagtaa
    23281 cgacgatatg gcgataggtg cgatgaaagc gttgcacgag cgcggcgtag cggtgccaga
    23341 gcaggtgtca gttatcggat tcgatgatat cgcaattgcc ccctatatcg ttccggcgct
    23401 ctccagcgta aaaattccgg tgactgagat gattcaggaa attattggac ggctgatttt
    23461 tatgctcgat ggcggggatt tctcaccgcc gaaaaccttc agcggaaaac tgatccgccg
    23521 cgactcactc attgctcttt cgcgataaac attgtcatca catgtcatcg tcactagtgt
    23581 cgatgacatc ttttttcctg cattacagaa attaatttcc ttaaataaca gtgaattaaa
    23641 actggcatga tttgtgaatg tatcggcgca ttaactgtca ttgctggaga atttgatgaa
    23701 ccgtttcatc attgctgacg cgagtaaatg tattggttgc tgtacctgtg aagtggcgtg
    23761 cgtggtttct catcaggaaa atcaggactg tgcatcgctg accccggaaa catttttacc
    23821 gcgtatccat gtcattaaag gtgtgaacat ttccacggcg acagtttgcc gtcagtgtga
    23881 agatgcaccg tgcgctaacg tctgcccgaa tggtgctatc agccgtgata aagggtttgt
    23941 tcatgtgatg caggaacgtt gcattggttg caaaacctgc gttgtggctt gcccgtatgg
    24001 cgcgatggaa gtggtggtac gtccggtgat tcgcaacagc ggcgcggggc tgaatgtacg
    24061 ggctgacaaa gccgaagcca ataaatgtga cctgtgcaac catcgtgaag acggcccggc
    24121 gtgtatggcg gcttgcccga ctcatgcgct gatttgtgtc gatcgtaata aacttgaaca
    24181 actgagtgca gaaaaacgcc gccgtacggc gctgatgttc taaaaatctt acagaactgt
    24241 tctggcatca aaggaattcc cggaaatatt cgtagaatac ggagctggcg gtatcgccga
    24301 cctgagactg ccggatacga cgcagtcgtg tccggcagga cgggttacgc aattcatgat
    24361 ggcgggaata actcaatggc aaaaaacaca tcttgcggtg tccaactgcg tattcgtggc
    24421 aaagtgcagg gcgtcggttt tcgtccgttt gtctggcagc tggcacagca attaaatctt
    24481 cacggcgatg tctgtaatga cggcgatggc gtagaagtcc ggctgcggga agacccggaa
    24541 acgttacttg ttcaattgca tcagcactgc ccgccgctgg cgcgtattga tagcgtcgag
    24601 cgtgagccgt acatctggtc acaactgccc actgagttca ccatccgcca gagcgcggga
    24661 ggtgccatga atacgcaaat tgtcccggat gccgctactt gccctgcttg ccttgccgaa
    24721 atgaataccc caggcgaacg gcgttatcgt tatccgttta tcaactgtac ccactgcggc
    24781 ccgcgtttca ccattattcg cgccatgcct tacgaccgcc cgtttaccgt gatggcggcg
    24841 tttccgttgt gtccggcttg tgacaaagag taccgtgacc cgctcgatcg tcgcttccac
    24901 gcccagccgg tggcctgccc ggagtgtggc ccacatcttg aatgggtaag tcatggtgaa
    24961 catgcagaac aagaggcggc attacaggcg gctatcgcac agttaaaaat gggcaacatt
    25021 gtcgccatca aagggattgg cggatttcat cttgcctgcg atgcacgtaa cagtaacgcg
    25081 gtggcgacac ttcgggcgcg caaacatcgc ccggcgaaac cgctggcggt catgttgcca
    25141 gtggctgacg gtttaccaga cgctgcgcgc cagttgctta ccacgcccgc cgcgccgatt
    25201 gtgctggtgg ataaaaaata cgttcctgag ctttgtgatg atatcgcccc tgaccttaac
    25261 gaagtcgggg taatgttgcc tgcgaacccg ctccagcatt tgctgttaca ggaactgcaa
    25321 tgcccgctgg tgatgacctc cggcaacctg agcggtaaac caccagctat cagcaacgaa
    25381 caggcgctgg cggatttgca gggcattgcc gacggattct tgatacataa ccgcgacatc
    25441 gtgcagcgga tggatgattc ggtggtgcgc gaaagcggcg aaatgctgcg ccgttcgcgg
    25501 gggtatgtgc cggatgcgct ggctttgcct ccgggcttta aaaatgttcc gcctgtgctg
    25561 tgtctcggcg cggatctgaa aaataccttc tgcctggtgc gcggtgaaca agcggtgttg
    25621 agtcagcatc tgggcgattt aagtgacgat ggcatccaga tgcagtggcg cgaagcgtta
    25681 cgcctgatgc aaaacatcta cgattttacc ccgcaatacg ttgtgcatga cgcgcatccg
    25741 ggctatgtct ccagccagtg ggcgcgtgaa atgaatctgc cgacgcaaac ggtactgcat
    25801 catcatgccc atgcagcggc gtgtctggca gagcatcagt ggccgttgga tggcggtgat
    25861 gtcattgctt tgacgctcga cggtatcggt atgggggaga acggcgcttt gtggggcggc
    25921 gagtgcctgc gggtgaacta tcgcgaatgt gagcacctgg gcggcttgcc tgcagtggcg
    25981 cttccgggtg gcgatttggc agcgaagcag ccgtggcgaa acctgctggc gcagtgcctg
    26041 cgctttgtgc cggagtggca gaattaccct gaaacagcaa gtgtgcaaca gcaaaactgg
    26101 agcgtgctgg cgcgggccat tgagcgtgga attaacgcgc cgctggcgtc atcgtgtggg
    26161 cggttgttcg atgcagtggc ggcggcactg ggctgtgcgc cagccacgtt aagttatgaa
    26221 ggtgaagcgg cttgtgctct ggaggcgctc gcagcctcat gccacggagt gacgcatccg
    26281 gtgacgatgc cgctggtgga caatcaactg gatctcgccg ctttctggca gcagtggctg
    26341 aactggcagg caccggttaa tcaacgcgcg tgggcgtttc atgatgcgct ggcgcagggt
    26401 tttgccgcgt tgatgcgtga gcaggccacg atgcgtggta tcactacgct ggtatttagc
    26461 ggcggggtta ttcataaccg tttgctgcgt gcacgtctgg cgcattatct cgctgatttc
    26521 acattgctct ttccacagag tttaccggcg ggtgatggcg gtttgtctct ggggcagggg
    26581 gttattgctg cggcgcgttg gttagcgggt gaagtccaga acggataaaa cgtgttgcat
    26641 ttggacacca ttgccggatg cggcgtaaac gccttatctg gcctacacat gcgtccccgt
    26701 aggtcggata agacgcgcca gcgtcgcatc cgacatttta ggcacagtag cccacctaca
    26761 tcggcaatgt tttcaacaat ccaaatgcct ctttcatccg atcctcactg accacaaagg
    26821 cgcgaagctg gtcagcatcg tcaacaccgc gagccaccat tccctggcgt tcggtattaa
    26881 tttgccagcg taaatcctga cgctgggttt cgcctgccag atgcagcggt aattccggcg
    26941 ttttgatttt caccagcatc gccggtagtt tcagcggcgt gttattgccg agaagatttt
    27001 ttgccagcac catcgcgcta agttgaatcg gctgcaggaa tggcagtacc tgaccgttga
    27061 tttccgcgca atcgcccagc gcataaatat cgtcattact ggtttgcaga taactatcga
    27121 cgcaaacgcc gcgattaatc gtcagcccgg cgcgtcgtgc cagggctgtt tccgggcgca
    27181 gtccggtggc ggcaattacc gcatccactt cgatactacg ctggtggtcc agcgttgcca
    27241 gaatgccaga atccgttttt tccagcccct gcaactgaga tttcaacagc agatgaacgc
    27301 ccatctccgt caaccgatgc tgcaagcggc tgcttacttc tggtggcatt aatgacgcca
    27361 gaatactggc agcgttgtcg attagcgtga ccgctttgcc tgcacgacaa aaatccatcg
    27421 ccagttcgct accaatcaaa ccaccgccga caatcaacac gcgtcgggca tcccgcagtt
    27481 gcgtttcaca ggcgcgatac tcttgctgac tatttaacgt cagcattaac tcacgcccag
    27541 gcactggcgg gacaaaggca ctggcaccag ttgccagcac cagcttgtcg tattgccact
    27601 gattattctg gcttttcacc acatgggctt cggcatcgat atccgtcacc caggtgtgcg
    27661 gaaacaggtg cagattaaac tgcccggcaa attcacccgc cgtctggcgg gtaaggtcgt
    27721 cggcacgttg cccctggctg ataacatggc tgaggtccgg tttgttgtac tcatccatgc
    27781 tgtcggcggc gatcagggtt aatggaatac tggcgtcctg tttgcgaata tttttcacca
    27841 gttggcgggc ggcgaagccc gaaccaatga tcacaatgcc gttgctcatt ttgcctccga
    27901 tgccagttcg tcaaagacgt ctttgccgag agagcattcc gggcagagga agttatccgg
    27961 aacttcactc cacggcgttc ctggcgcaac gtcctgcatt ggctcgcctt ttgccggatc
    28021 gtaaatccac tggcagacgc tgcactgcat ccgtgggccg aggtcggact tcgtggtggc
    28081 agaggtttct tctttaacca ccgtattcac cgtgctctgc ggcagcggcg cgagcgccca
    28141 ctgacgggcg atttcgcgac catgttcacg gcataactcc agagcgtcct ggtctggtcg
    28201 ccatttggct ttcaggctaa gcgacatttc gaaacccgca tcctgcaggc gcgtggaaag
    28261 acgatccacc gcaccgccgc tccagccgtg agagccgaaa gcactggcgc gtttgttacg
    28321 gaagcgtaat ccggtcatct cctccaccag accggcgatt ttcggcatca tcacgttatt
    28381 catcgtcgaa gtgccgacca gcacgccttt tgagcggaag acattggtca ggatttcgtt
    28441 tttatcgctt cgggcgacgt tgaaaatttt caccgccacg cgtgggtcgg tttccgcaat
    28501 cccctgggcg atagcgtcag ccatcatgcg ggtgttattc gacatggtgt cgtagaaaat
    28561 ggtgattctg tcttcctgat aatcagccgc ccatttcagg tacagctcga caatttgcgt
    28621 cgggttatcg cgccatacca cgccgtgaga ggtcgctatc atatcgactg gtaagttaaa
    28681 gcccaggatc tcggtaattt tcggtgttac caggcggctg aacggcgtca ggatattggc
    28741 gtagtaacgc tggcactgct cgaaaagctc cgtctgatcc acttcatcgt tgaacagatg
    28801 ctcgtcgcag tagtgctgac cgaaagcatc gttactgaac agcaccgcgt cgcctgtcag
    28861 gtaagtcatc atgctgtccg gccagtgcag cattggcgtt tcgacaaaaa tgagctgttt
    28921 gccgttgccg atatccagcg tgtcaccagt tttcaccaca ttaaaattcc actccggatg
    28981 atgatgatga ccatttatcg agtcgatagc gttggcagta cagtaaatcg gcgtatcggg
    29041 aatttgcgcc atcagttcgg tcagcgcccc agcgtggtcc tcttctgcat ggttaatcac
    29101 gatgtaatcg atatccgcca gatcgatttc attacgcagg ttctgcacaa attcgcggct
    29161 gaatttatgg tcgacggtgt cgatcagcac gtttttttct tcgcggatga ggtagctgtt
    29221 gtaactgctg ccgcgtagcg ttttatattc cgtaccgtga aaatcacgca cttcccagtc
    29281 acgttgacca acccaatgaa tgttattttt caccacaata gacatagcaa cctcaattta
    29341 ttcagcgtgt tctaaaaaga tgtcttgcta attgcagatt acgtgccagt tttttatctg
    29401 attgttttta ctctataaat aaaattatgc tcattaatga gtagtcaaaa tgactatcaa
    29461 tcaaatggtc gatatgacaa tatctatagt caaattgaca gtgaggcaaa gatgagtttt
    29521 tccgttgatg tgctggcgaa tatcgccatc gaattgcagc gtgggattgg tcatcaggat
    29581 cgttttcagc gcctgatcac cacgctacgt caggtgctgg agtgcgatgc gtctgcgttg
    29641 ctacgttacg attcgcggca gtttattccg cttgccatcg acggtctggc aaaggatgta
    29701 ctcggtagac gctttgcgct ggaagggcat ccacggctgg aagcgattgc ccgcgccggg
    29761 gatgtggtgc gctttcccgc agacagcgaa ttgcccgatc cctatgacgg tttgattcct
    29821 gggcaggaga gtctgaaggt tcacgcctgc gttggtctgc cattgtttgc cgggcaaaac
    29881 ctgatcggcg cactgacgct cgacgggatg cagcccgatc agttcgatgt tttcagcgac
    29941 gaagagctac ggctgattgc tgcgctggcg gcgggagcgt taagcaatgc gttgctgatt
    30001 gaacaactgg aaagccagaa tatgatgcca ggcgatgcca cgccgtttga agcggtgaaa
    30061 cagacgcaga tgattggctt gtcccctggc atgacgcaac tgaaaaaaga gattgagatt
    30121 gtggcggcgt ccgatctcaa cgtcctgatc agcggtgaga cgggaaccgg taaggagctg
    30181 gtggcgaaag cgattcatga agcctcgcca cgggcggtga atccgctggt ctatctcaac
    30241 tgtgctgcac tgccggaaag tgtggcggaa agtgagttgt tcgggcatgt gaaaggagcg
    30301 tttactggcg ctatcagtaa ccgcagcggg aagtttgaaa tggcggataa cggcactctg
    30361 tttctggatg agatcggcga gttgtcgttg gcattgcagg ccaagctgct gagggtgttg
    30421 cagtatggcg atattcagcg cgttggcgat gaccgtagtt tgcgggtcga tgtgcgcgtg
    30481 ctggcggcga ctaaccgcga cttacgcgaa gaggtgatgg cagggcgatt tcgcgctgac
    30541 ttgtttcatc gcctgagcgt gtttccactt tcggtgccgc cgctgcgtga gcggggcgat
    30601 gatgtcattc tgctggcggg gtatttctgc gagcagtgtc gtttgcggct ggggctctcc
    30661 cgcgtggtat taagtgccgg agcgcgaaat ttactgcaac actatcgttt tccggggaac
    30721 gtgcgcgaac tggaacatgc tattcatcgg gcggtagtgc tggcgagagc cacccgcaac
    30781 ggcgatgaag tgattcttga ggcgcaacat tttgcttttc ctgaggtgac gttgccgccg
    30841 ccagaagcgg cggcggtgcc cgttgttaag caaaacctgc gtgaagcgac agaagcgttc
    30901 cagcgtgaaa ctattcgcca ggcactggca caaaatcatc ataactgggc tgcctgcgcg
    30961 cggatgctgg aaaccgacgt cgccaacctg catcggctgg cgaaacgtct gggaatgaag
    31021 gattaaataa tcccggcctg atagaaatcc tgcaggttaa ttgcgccggt gagtttgccg
    31081 ttttcatcca ccaccggtgc ggcagtgatt ttgcgcttca tcaggatctc tttggcgtcg
    31141 atggcgcgac tttgcgcctg taacgtggtg ccgcctgtcg tcatcgcttc attaactggc
    31201 gtggtgagtg cgccgccgcc taccagccag cgacgtaaat cgccgtcggt aaagacgcct
    31261 tgtacctgtt gttgagcgtc acataccgcc accagcccca gaccggtgcg gctgagttcc
    31321 agcatcgcat ccatcacgct ggcggttaac gccacctgtg ggatggcatc gtcacggcgc
    31381 atcagatgat gcactttatt cagcaagcga gcgcccagtg ccccggctgg gtgggagcgg
    31441 gcaaaatctt cttcattaaa tccgcgcgcc tgcatgaccg ccatcgccag cgcgtcaccc
    31501 atcatcaggg tattgacggt gctggaggtc ggcgcaaggt gcatcgggca ggcttcgcgt
    31561 tctacggaga tatccagcac cgcttttgcc gccaggccca gcggtgacgt cggtttgccg
    31621 gtcatcgcca gcagcgcgat agatttatct tccagacgcg gaataatcag atccagttcc
    31681 ttcgcgccac cggagtaaga gataaacagc atcacatcgc ggctttcaat catccccaga
    31741 tcgccgtgca gtgcttctgc cggatggaca aaaaaagccg atgtgccggt actggcaagt
    31801 gttgcggcga ttttcttacc aatgtggccc gatttgccaa ttcccgaaac caccactttg
    31861 ccttcgcagt gcaggatgat attggcggcg cgaacaaaat catcgcccag acgttccggt
    31921 aaacggcttg cttcctgcaa ctccagcatt aacgtctgac gtcccgcgtt cagtagtgct
    31981 tcactcattg ctctctccgg ttatgatcac atcgatccct ttctcttcca gcgcctgacg
    32041 aaacgccgga tcgatacctg cgtcggtaat cagcttatcg acgctttcaa gactgcaaac
    32101 tacgttgggg cttttacggc caaactttga tgagtccgcc atcaaaatca cttcgcgcgc
    32161 ggcattgcac attgccttac tgacggtata aacctcgtta aaggtggtta cgcccgcatt
    32221 gagatcgatg ccgtcggtgc ccataaacaa tttatcgaaa ctgaaatgtt cgaaggcatt
    32281 ctctgccagc tgtccgtgaa atgaggccga ttttttacga aacgttccgc ctggcatcag
    32341 gatagtttgt tcgttatcca gttcagatag cgcattgacg atatgcagac tgttggtcat
    32401 caccgtgatg ttattaaagc gcgagagcag gggaaccatc tgcaaaacgg tactgccagc
    32461 atcaagaatg atcgaatcgc catcatgaat aaaactaacg gcagcttctg ctatcagctc
    32521 tttcttgtgg gtgttgatga gtgttttatg atcgataggc ggatcggatt cctctttatt
    32581 caacaccact ccgccataag tacgaatgac ggttcctgca tgttccagaa tgaccagatc
    32641 tttgcgaatg gttgtgcctg tggtgtcaaa gtattgcgcc aattcttcaa ccgagcattt
    32701 accctgcttt tgcagatact ccagaatggc ggcctgacgc tgacgaggtt tcatgggcgt
    32761 gattacctga tttaggtttc aaaatgataa cttcgcaagc aagtaacatt cacaacgaaa
    32821 ttatccacgt ttcagtttaa gcgccaatga tagagcattc tgtgatagtg gatcatgggg
    32881 aaagatcaca tcgggctgta aatcacccgc atgcataccg gtaattgcgg gaattttttg
    32941 cggtcgtgcg aagacggtca gtcctttcat aaacaaggtg tcgacgatgc gctgctgatc
    33001 gtccagggcg atggcgacca cgacccgtgg tttaaagcgc ccgctggaac ggcccacgcc
    33061 aacccgccct tgctggcata gtgtgtcgaa ggcacggtta aaacgagaaa tttgccagcc
    33121 gcctaaggcc agttgcgcac accaggcgat aacggcgacg gtgatgagtg cggataccat
    33181 aacttttcct taatgtagtg gcggaagtcc tgtatgcctg atgagcgtag cgcatcaggc
    33241 attctattag tctcacggag atccttaacg gatctccgct gttgattaca acatcacctg
    33301 accgccggtg acattgatcg actgtccggt gcagtacgac gccttaggac tggcatagag
    33361 cagcagcata ttcagcacat cctgataatc gcagccgcgt ttgagcggta ctttgtcgat
    33421 gtaatactgc tcgacttgat ccggtttgat acccagcttg gtcgcgtatt ggggtaacag
    33481 tgactggaac ataggcgatt tcagcaggtt accgagcatc agtgaatgca ccgtaatgcc
    33541 gtactccgcc agatccagcg ccagtgattg agtcagcccg acgccaccaa atttcgctgc
    33601 gctgtagcca gagttgtgtt tgctgcccac tttgccggat ttcgagttga tctgaataat
    33661 gcgaccctga atcccgtcgc ggatcatcaa acgagaaaat tcacgggcac acaggaaata
    33721 acccaccaga ttcacggtca aaatcgccga gctggaagtc gctgataaag gctgcttttg
    33781 ctattccggc gctgtagacc agcaaatcca cgcgcccaaa gatttcatct accccacgag
    33841 agagcgccag gacgctttgc tcgctggtgg cgtcagcacc aaaactatac gctgttcctt
    33901 caccatattc ggcgttaatt tcttgtgcca catttgcggc tttgtcgctc tgaatatcga
    33961 caaccgcgac gcgatacccc tcggcagcca gaccgtggca caggaacgcg cctaaggttt
    34021 gcccaccacc gatgacaacg gcaacctgat tcatttttta ctccttaaca gattcaaact
    34081 tcaaaaccga tcccggcgcg atatcgtcgg gtacagggcc tgccacatgg acagtgcccg
    34141 gaaattccgc ttcgtttaaa ccatcgaagc gcagggtgac atgacccagt tcgcgaaggt
    34201 tgtcttccgc cacgctgcca acagcggtca ccggatagcg atgctgcccg agcgtaaatt
    34261 gcaaaccggg atggagtgca cctttcaact cgccgtggca atgaatgaag caatactctt
    34321 cgaggtccgc tggcgcgcct tcacgaaagg tgatgagcat ctggtcgctg agggcgtcag
    34381 tggcactcgc gccgatacgg gtgatggtgg tctgataaat aacggtcatg tttcagcctc
    34441 tattgataga taaaaccgga gacaaaccag gcgatcagta cagtcggtgc gccggttaaa
    34501 aagtggctca ccagtacaga agggacaccg acgcgaaccg tgtcctgacg ggcttccgcc
    34561 agcgacaaac cgaccgggat gaagtcgcag gccgcctgcg cgttgatggc aaacagtgcc
    34621 ggtaaagcca gatgcggcgg aatattgccg agaccaatct gcacgccaat caatacgccg
    34681 ataacctgtg cgataactgc gcctgggccg aggaaaggtg aaagcagcgg gaaggagcag
    34741 atgagcgcca gcatgaccag acctagtgga tggctcgcca gcggagcaag accgtgggca
    34801 atccagtcac caaggccaga agccataatg atgccaatga gcgccgagac gaacgccata
    34861 aacggcagaa tggtttttaa tacagtgtcg atggtgtcac gaccagattg aaacaacacg
    34921 gcaacggcag aacccatacc cattcccacc ttcgccagta aaccatcgct ttgttcggtg
    34981 attttcttgc tggtgtcata gtcacggccc acggaagagg gttgtggtgt cgcatcaccc
    35041 actacagtga tgttttcttc ttttacgcca gagacataaa tatcttccac aatgtactgc
    35101 gccagcggac tggacttgcc cgtcgagtgg atattaatgg tgggaatacg tcgtttcgga
    35161 tagatgccgc agcgtaatgt gccgccacag tcgattaccg cgacaccaat ttccgcctcc
    35221 gcgggttcac cttctttaaa tccgtcaata gcctgccagc cagtaagttg tgccagtttg
    35281 tcaacaatcg caggccgggt accggcggtg atatagacga tttttttgcc cggcgtggct
    35341 tccagctcaa gcgggccgcc ccagccaccc gttccttttt cgatccgaat acgcgtcatg
    35401 atgttgctcc tgccaggtga actttttgtt caagttgaat gcccatcttt ttctcaaaaa
    35461 tggcggtggt cagatcggtc acccagccgc gaaagaagtt ggtgaccaga ccaaccagca
    35521 gatagctcac cgccagtggg ccaagtggca ggttcagcgt tgtcagaccg ctggcaatgc
    35581 caagataaac aaacagttcg ccagggttga tatggggaaa gaggccattc attgagtggc
    35641 agctataaga ggctgccgcg tagtagctgg gtttgtactt ttccggcata aagcgaccca
    35701 ggcttaaggt catcggattg caaaagacaa acgtgccaat gcacggtagc agcaggtaac
    35761 gggaaacagg gttaccggcg caacgttgag caaaacgttc aatacgatgc tgaccgataa
    35821 aattaatcag tgcgttcatg ataaccagca ggctaatcaa cagcggaaga atgccggtca
    35881 ccatcccggt aaacacctct ccgccctttt ggaacagccc gataaaccac tctgcaccat
    35941 gagtaatggt ttctatcatt gttctctcct tcaggattta ttgttttatt accaaacggc
    36001 aacctaatct aatcagattg aaagatttga aagtgttatt ttgatcgcaa aatgaaagat
    36061 aaatatttta atttgaaagt ttgaataaaa ggatagcgag gggaatgagt tgagttatgt
    36121 aaagtccgta tcgggcagtg actaccgctt ccttgtgcgg ggcgtgatgc tttaccatac
    36181 ttgcccctgg ttgaatctgt taaatggacc cctcatgttc aagcgtcgtt atgtaacatt
    36241 gcttcccctt tttgtgttgc ttgccgcctg tagcagcaag ccaaaaccta ctgagactga
    36301 aacgaccacc ggaacgccgt ctggcggctt cctgcttgag cctcagcaca atgtgatgca
    36361 gatgggcggc gatttcgcta ataacccgaa tgcccagcag ttcatcgaca gaatggtgaa
    36421 caaacacggt ttcgatcgtc agcagttgca ggaaattctc tcccaggcga agcgtctgga
    36481 ttcggtactg cggctgatgg ataaccaggc accaaccaca tcggtgaaac cgccatcagg
    36541 tccgaacggc gcatggctgc gttatcgcaa aaaatttatt acgccggaca acgtgcagaa
    36601 cggtgtggtt ttctggaatc agtatgaaga tgcgttgaat cgcgcgtggc aggtgtatgg
    36661 cgtaccgccg gaaattatcg tcgggattat cggcgttgaa acccgctggg ggcgcgtgat
    36721 ggggaaaact cgcatcctcg atgcgctggc aacgctgtca tttaactacc cacgccgcgc
    36781 ggagtatttc tctggcgagc tggaaacctt cctgttgatg gcgcgcgacg agcaggacga
    36841 tccgctcaat ctgaaaggtt cctttgccgg ggcgatgggc tacggacagt ttatgccgtc
    36901 gtcttacaaa caatatgcgg tagatttcag cggcgacggg catatcaacc tgtgggatcc
    36961 ggttgatgcg atcggtagtg tggcgaacta tttcaaagcg cacggctggg tgaaaggcga
    37021 tcaggtcgcg gtaatggcaa acggtcaggc tccaggcttg ccaaatggct tcaaaaccag
    37081 gtacagcatt tcgcagcttg ccacggcagg tttaacgcca cagcagccgc tgggcaacca
    37141 tcaacaagcc agcctgctgc gtctggatgt cggtaccggt taccagtact ggtacggtct
    37201 gccgaacttc tacaccatta cccgttataa ccacagcacc cattacgcaa tggcggtctg
    37261 gcagttagga caagccgtgg cgctggcgcg cgtgcagtaa ctatttgcaa gaggggggcg
    37321 tgagcttccc tctggctaat cagctttttt ctgaatcctc ctcgtaaaat tgcaacgcca
    37381 acaccatctt cctgacgaaa gtgctatctt gtccggcata aatttttgac tgactgaggt
    37441 tgtgatgact gacagtgaac tgatgcagtt aagcgaacag gttgggcagg cgctgaaagc
    37501 ccgtggcgca accgtaacaa ctgccgagtc ttgtaccggt ggttgggtag cgaaagtgat
    37561 taccgatatt gccggtagct ccgcctggtt tgaacgcgga tttgtcacct acagtaacga
    37621 agccaaagcg cagatgatcg gcgtacgcga agagacgctg gcgcagcatg gcgcggtgag
    37681 tgaacccgtc gtggtggaaa tggcgatagg tgcactgaaa gcggctcgtg ctgattatgc
    37741 cgtgtctatt agtggtatcg ccgggccgga tggcggcagt gaagagaagc ctgtcggcac
    37801 cgtctggttt gcttttgcca ctgcccgcgg tgaaggcatt acccggcggg aatgcttcag
    37861 cggcgaccgt gatgcggtgc gtcgtcaggc tactgcgtat gcattgcaga ccttgtggca
    37921 acaatttcta caaaacactt gatactgtat gagtatacag tataattgct tcaacagaac
    37981 atattgacta tccggtatta cccggcatga caggagtaaa aatggctatc gacgaaaaca
    38041 aacagaaagc gttggcggca gcactgggcc agattgagaa acaatttggt aaaggctcca
    38101 tcatgcgcct gggtgaagac cgttccatgg atgtggaaac catctctacc ggttcgcttt
    38161 cactggatat cgcgcttggg gcaggtggtc tgccgatggg ccgtatcgtc gaaatctacg
    38221 gaccggaatc ttccggtaaa accacgctga cgctgcaggt gatcgccgca gcgcagcgtg
    38281 aaggtaaaac ctgtgcgttt atcgatgctg aacacgcgct ggacccaatc tacgcacgta
    38341 aactgggcgt cgatatcgat aacctgctgt gctcccagcc ggacaccggc gagcaggcac
    38401 tggaaatctg tgacgccctg gcgcgttctg gcgcagtaga cgttatcgtc gttgactccg
    38461 tggcggcact gacgccgaaa gcggaaatcg aaggcgaaat cggcgactct cacatgggcc
    38521 ttgcggcacg tatgatgagc caggcgatgc gtaagctggc gggtaacctg aagcagtcca
    38581 acacgctgct gatcttcatc aaccagatcc gtatgaaaat tggtgtgatg ttcggtaacc
    38641 cggaaaccac taccggtggt aacgcgctga aattctacgc ctctgttcgt ctcgacatcc
    38701 gtcgtatcgg cgcggtgaaa gagggcgaaa acgtggtggg tagcgaaacc cgcgtgaaag
    38761 tggtgaagaa caaaatcgct gcaccgttta aacaggctga atttcagatc ctctacggcg
    38821 aaggtatcaa cttctacggc gaactggttg acctgggcgt aaaagagaag ctgatcgaga
    38881 aagcaggcgc gtggtacagc tacaaaggtg agaagatcgg tcagggtaaa gcgaatgcaa
    38941 ctgcctggct gaaagataac ccggaaaccg cgaaagagat cgagaagaaa gtacgtgagt
    39001 tgctgctgag caacccgaac tcaacgccgg atttctctgt agatgacagc gaaggcgtag
    39061 cagaaactaa cgaagatttt taatcgttcg ttattgatga taatagataa tgtgtgcacg
    39121 gacggtcccc tcgccccttt ggggagaggg ttagggtgag gggaacaggc caacactggc
    39181 gtgaaaaatt actctcacct cgatcctcta acgaattggc tgcacactca ttgactatta
    39241 aatctgttta tcagcaaaac ctgggtcgca tctgcggccc ttttgctttt ttaagttgta
    39301 aggatatgcc atgacagaat caacatcccg tcgcccggca tatgctcgcc tgttggatcg
    39361 tgcggtacgc attctggcgg tgcgcgatca cagtgagcaa gaactgcgac gtaaactcgc
    39421 ggcaccgatt atgggcaaaa atggcccaga agagattgat gctacggcag aagattacga
    39481 gcgcgttatt gcctggtgcc atgaacatgg ctatctcgat gacagccgat ttgttgcgcg
    39541 ctttatcgcc agccgtagcc gcaaaggtta tggacctgca cgtattcgtc aggaactgaa
    39601 tcagaaaggt atttcccgcg aagcgacaga aaaagcgatg cgtgaatgtg acatcgactg
    39661 gtgcgcactg gtgcgcgatc aggcgacgcg aaaatatggt gaacctttgc caactgtctt
    39721 ttcagaaaaa gttaagatcc agcgttttct gctctatcgt ggctatctga tggaagatat
    39781 ccaggatatt tggctaaatt ttgccgactg aacgcatacg gtattttact tcccactcaa
    39841 gaaaacttat cttattccca cttttcagtt accagcccgg cggttaagac acgctggagc
    39901 tggtggcgat atttcgttag cttgatttca ggataattat gagcaagagc accgctgaga
    39961 tccgtcaggc gtttctcgac tttttccata gtaagggaca tcaggtagtt gccagcagct
    40021 ccctggtacc ccataacgac ccaactttgt tgtttaccaa cgccgggatg aaccagttca
    40081 aggatgtgtt ccttggactc gacaagcgta attattcccg cgctaccact tcccaacgct
    40141 gcgtgcgtgc gggtggtaaa cacaacgacc tggaaaatgt cggttacacc gcgcgtcacc
    40201 ataccttctt cgaaatgctg ggcaacttca gcttcggcga ctatttcaaa cacgatgcca
    40261 ttcagtttgc atgggaactg ctgaccagcg aaaaatggtt tgccctgccg aaagagcgtc
    40321 tgtgggttac cgtctatgaa agcgacgacg aagcctacga aatctgggaa aaagaagtag
    40381 ggatcccgcg cgaacgtatt attcgcatcg gcgataacaa aggtgcgcca tacgcatctg
    40441 acaacttctg gcagatgggt gacactggtc cgtgcggccc gtgcaccgaa atcttctacg
    40501 atcacggcga ccacatttgg ggtggccctc cgggaagtcc ggaagaagac ggcgaccgct
    40561 acattgagat ctggaacatc gtcttcatgc agttcaaccg ccaggcagat ggcacgatgg
    40621 aaccgctgcc gaagccgtct gtagataccg gtatgggtct ggagcgtatt gctgcggtgc
    40681 tgcaacacgt taactctaac tatgacatcg acctgttccg cacgttgatc caggcggtag
    40741 cgaaagtcac tggcgcgacc gatctgagca ataaatcgct gcgcgtaatc gctgaccaca
    40801 ttcgttcttg tgcgttcctg atcgcggatg gcgtaatgcc gtccaatgaa aaccgtggtt
    40861 atgtactgcg tcgtatcatt cgtcgcgcag tgcgtcacgg caatatgctc ggcgcgaaag
    40921 aaaccttctt ctacaaactg gttggtccgc tgatcgacgt tatgggctct gcgggtgaag
    40981 acctgaaacg ccagcaggcg caggttgagc aggtgctgaa gactgaagaa gagcagtttg
    41041 cccgtactct ggagcgcggt ctggcgttgc tggatgaaga gctggcaaaa ctttctggtg
    41101 atacgctgga tggtgaaacg gctttccgtc tgtacgacac ctatggcttc ccggttgacc
    41161 tgacggctga tgtttgtcgt gagcgcaaca tcaaagttga cgaagctggt tttgaagcag
    41221 caatggaaga gcagcgtcgt cgcgcgcgcg aagccagcgg ctttggtgcc gattacaacg
    41281 caatgatccg tgttgatagt gcatctgaat ttaaaggcta tgaccatctg gaactgaacg
    41341 gcaaagtgac cgcgctgttt gttgatggta aagcggttga tgccatcaat gcaggccagg
    41401 aagctgtggt cgtgctggat caaacgccat tctatgcgga atccggcggt caggttggtg
    41461 ataaaggcga actgaaaggc gctaacttct ccttcgcggt ggaagatacg cagaaatacg
    41521 gccaggcgat tggtcacatc ggtaaacttg ctgcgggttc tctgaaagtg ggcgacgctg
    41581 tgcaggctga tgttgatgag gctcgtcgcg cccgtattcg tctgaatcac tccgcaacgc
    41641 acctgatgca cgctgcgctg cgccaggttc tggggactca tgtatcgcag aaaggttcac
    41701 tggttaacga caaggtgctg cgcttcgact tctcacacaa cgaagcgatg aaaccggaag
    41761 agattcgtgc ggtcgaagac ctggtgaacg cacagattcg tcgcaatttg ccgatcgaaa
    41821 ccaacatcat ggatctcgaa gcggcgaaag cgaaaggtgc gatggcgctg ttcggcgaga
    41881 agtatgatga gcgtgtacgc gtgctgagca tgggcgattt ctccaccgag ctgtgtggcg
    41941 gtactcacgc cagccgcact ggtgatattg gtctgttccg catcatctct gaatcgggta
    42001 ctgctgcagg cgttcgtcgt atcgaagcgg taaccggaga aggcgctatc accaccgttc
    42061 atgcagacag cgatcgctta agcgaagtcg cgcatctgct gaaaggcgat agcaataatc
    42121 tggcggataa agtgcgctca gtactggaac gtacgcgtca gttagaaaaa gaactacaac
    42181 agcttaaaga acaagctgcc gcacaggaga gcgcaaatct ttccagtaag gcaattgatg
    42241 ttaatggtgt taagctgttg gttagcgagc ttagcggtgt tgagccgaaa atgttgcgta
    42301 ccatggttga cgatttaaaa aatcagctgg ggtcgacaat tatcgtgctg gcaacggtag
    42361 ccgaaggtaa ggtttctctg attgcaggcg tatctaagga cgtcacagat cgtgtgaaag
    42421 caggggaact gattggtatg gtcgctcagc aggtgggcgg caagggtggt ggacgtcctg
    42481 acatggcgca agccggtggt acggatgctg cggccttacc tgcagcgtta gccagtgtga
    42541 aaggctgggt cagcgcgaaa ttgcaataat ataagcgtca ggcaatgccg tggactcgct
    42601 tcacggcatt cgcattaacg ctatcgacaa cgataaagtc aggttgaagt tgtgtatatc
    42661 ggctaaactt aggtttaaca gaatgtaatg ccatgactgc ttacatgtaa tgtgtttgtc
    42721 attgcttact ttttggcgtt atatgatgga taatgccggg atacagagag acccgactct
    42781 tttaatcttt caaggagcaa agaatgctga ttctgactcg tcgagttggt gagaccctca
    42841 tgattgggga tgaggtcacc gtgacagttt taggggtaaa gggcaaccag gtacgtattg
    42901 gcgtaaatgc cccgaaggaa gtttctgttc accgtgaaga gatctaccag cgtatccagg
    42961 ctgaaaaatc ccagcagtcc agttactaat ctttccgcgt ctcatcttta tcggtgagac
    43021 gcaccctcaa aatttctcct tcactctata gtcttttcgc tttactcccg ttcattcaac
    43081 ttaagtctcc atttttttgc attactgcta tctgccagac ctccgttttt ctgtagataa
    43141 aacactcttt ttgacgtttt tacagactaa ttgagcgtga agtgtgcaaa cgataaaagt
    43201 gtgggaaaaa ttgtttgact tataagtctc agaaagtaat atgtgcgcca cgcagcgacg
    43261 atgagcgata aacaagttct tcgaagcact cgtaagaggc gtgtggtgag gtggccgaga
    43321 ggctgaaggc gctcccctgc taagggagta tgcggtcaaa agctgcatcc ggggttcgaa
    43381 tccccgcctc accgccattt gcatccgtag ctcagctgga tagagtactc ggctacgaac
    43441 cgagcggtcg gaggttcgaa tcctcccgga tgcaccatct cttacttgat atggctttag
    43501 tagcggtatc aatatcagca gtaaaataaa ttttcccgat gcatccgtag ctcagctgga
    43561 tagagtactc ggctacgaac cgagcggtcg gaggttcgaa tcctcccgga tgcaccacct
    43621 cttacttgat atggctttag tagcggtatc aatatcagca gtaaaataaa ttttcccgat
    43681 gcatccgtag ctcagctgga tagagtactc ggctacgaac cgagcggtcg gaggttcgaa
    43741 tcctcccgga tgcaccatat tctccgaaat cttcagcaat gaaggtatcg aagaggtagc
    43801 ggaattaacc gcaggcacca gggatgataa cgttgcttta gcaacggccc gaagggcgag
    43861 ccgcaaggcg agtaatcctc ccggatgcac catctcttaa ttgatatggc tttagtagcg
    43921 gtatcaatat cagcagtaga ataagttttc ccgatgcatc cgtagctcag ctggatagag
    43981 tactcggcta cgaaccgagc ggtcggaggt tcgaatcctc ccggatgcac catattctcc
    44041 gtaaccttca gcaatgaagg tatcgaagag atagcggtat taaccgcagg caccaggaag
    44101 gataacgttg cgccagcaac ggcccgaagg gcgaacgtca gtgagtcatc ctcccggatg
    44161 caccatcttc tccaaaaaat ttccttcaaa aatatctcat ttctaaataa ctcctttatc
    44221 catttcctga attttcttct ccttcgccat cagcgacaaa atcaagcaat tacgctaaag
    44281 tagcgtcata tttttcgact tgcgagaaga ccatgtacga gcgttatgca ggtttaattt
    44341 ttgatatgga tggcacaatc ctggatacgg agcctacgca ccgtaaagcg tggcgcgaag
    44401 tattagggca ctacggtctt cagtacgata ttcaggcgat gattgcgctt aatggatcgc
    44461 ccacctggcg tattgctcag gcaattattg agctgaatca ggccgatctc gacccgcatg
    44521 cgttagcgcg tgaaaaaaca gaagcagtaa gaagtatgct gctggatagc gtcgaaccgc
    44581 ttcctcttgt tgatgtggtg aaaagttggc atggtcgtcg cccaatggct gtaggaacgg
    44641 ggagtgaaag cgccatcgct gaggcattgc tggcgcacct gggattacgc cattattttg
    44701 acgccgtcgt cgctgccgat cacgtcaaac accataaacc cgcgccagac acatttttgt
    44761 tgtgcgcgca gcgtatgggc gtgcaaccga cgcagtgtgt ggtctttgaa gatgccgatt
    44821 tcggtattca ggcggcccgt gccgcaggta tggacgctgt ggacgttcgc ttgctgtgag
    44881 tgaagcgtta tcgcttttct cgttgtttgc cagtagtttt ctcagcgcta cactattacc
    44941 cggcaactcc gaagtcgtgc tggtggcaat gttgctttcc gggatcagtc atccctgggt
    45001 tttagtttta acagcaacaa tgggtaatag ccttggaggg ttaactaacg ttatccttgg
    45061 gcgtttcttt ccattgcgta aaacatcgcg ctggcaagag aaagctatcg gctggctgaa
    45121 acgctatggt gcagtcacgc tattattaag ctggatgccc gtggttggcg atttactgtg
    45181 tctgttagcg ggatggatgc gcatctcgtg gggaccggta atcttttttt tgtgccttgg
    45241 taaagcgtta cgctatgttg cagttgcagc agcgaccgtt cagggcatga tgtggtggca
    45301 ctaattgtag gcctgcacat atggtcacca ttacagttat gctaattaaa acgattttga
    45361 caggcgggag gtcaatttga tcccggacgt atcacaggcg ctggcctggc tggaaaaaca
    45421 tcctcaggcg ttaaagggga tacagcgtgg gctggagcgc gaaactttgc gtgttaatgc
    45481 tgatggcaca ctggcaacaa caggtcatcc tgaagcatta ggttccgcac tgacgcacaa
    45541 atggattact accgattttg cggaagcatt gctggaattc attacaccag tggatggtga
    45601 tattgaacat atgctgacct ttatgcgcga tctgcatcgt tatacggcgc gcaatatggg
    45661 cgatgagcgg atgtggccgt taagtatgcc atgctacatc gcagaaggtc aggacatcga
    45721 actggcacag tacggcactt ctaacaccgg acgctttaaa acgctgtatc gtgaagggct
    45781 gaaaaatcgc tacggcgcgc tgatgcaaac catttccggc gtgcactaca atttctcttt
    45841 gccaatggca ttctggcaag cgaagtgcgg tgatatctcg ggcgctgatg ccaaagagaa
    45901 aatttctgcg ggctatttcc gcgttatccg caattactat cgtttcggtt gggtcattcc
    45961 ttatctgttt ggtgcatctc cggcgatttg ttcttctttc ctgcaaggaa aaccaacgtc
    46021 gctgccgttt gagaaaaccg agtgcggtat gtattacctg ccgtatgcga cctctcttcg
    46081 tttgagcgat ctcggctata ccaataaatc gcaaagcaat cttggtatta ccttcaacga
    46141 tctttacgag tacgtagcgg gccttaaaca ggcaatcaaa acgccatcgg aagagtacgc
    46201 gaagattggt attgagaaag acggtaagag gctgcaaatc aatagcaacg tgctgcagat
    46261 tgaaaacgaa ctgtatgcgc cgattcgtcc aaaacgcgtt acccgcagcg gcgagtcacc
    46321 ttctgatgcg ctgttacgtg gcggcattga atatattgaa gtgcgttcgc tggacatcaa
    46381 cccgttctcg ccgattggtg tagatgaaca gcaggtgcga ttcctcgacc tgtttatggt
    46441 ctggtgtgcg ctggctgatg caccggaaat gagcagtagc gaacttgcct gtacgcgcgt
    46501 taactggaac cgggtgatcc ttgaaggtcg caaaccgggc ctgacgctgg gtatcggctg
    46561 cgaaaccgcg cagttcccgt tactgcaggt gggtaaagat ctgttccgcg atctgaaacg
    46621 cgtcgcgcaa acgctggata gcattaacgg cggcgacgcg tatcagaaag tgtgtgatga
    46681 actggttgcc tgcttcgata atcccgatct gactttctct gcccgtatct taaggtctat
    46741 gattgatact ggtattggcg gaacaggcaa agcgtttgca gaagcctacc gtaatctgct
    46801 gcgtgaagag ccgctggaaa ttctgcgcga agaggatttt gtagccgagc gcgaggcatc
    46861 tgaacgccgt cagcaggaaa tggaaaccgc tgataccgaa ccgtttgcgg tgtggctgga
    46921 aaaacacacc tgacagaaaa gaaaaaggcc actcgtgagt ggccaaaatt tcatctctga
    46981 attcagggat gatgataaca aatgcgcgtc tttcatatac tcagactcgc ctgggaagaa
    47041 agagttcaga aaatttttaa aaaaattacc ggaggtggct aaatgccgtt gttagatagc
    47101 ttcacagtcg atcatacccg gatggaagcg cctgcagttc gggtggcgaa aacaatgaac
    47161 accccgcatg gcgacgcaat caccgtgttc gatctgcgct tctgcgtgcc gaacaaagaa
    47221 gtgatgccag aaagagggat ccataccctg gagcacctgt ttgctggttt tatgcgtaac
    47281 catcttaacg gtaatggtgt agagattatc gatatctcgc caatgggctg ccgcaccggt
    47341 ttttatatga gtctgattgg tacgccagat gagcagcgtg ttgctgatgc ctggaaagcg
    47401 gcaatggaag acgtgctgaa agtgcaggat cagaatcaga tcccggaact gaacgtctac
    47461 cagtgtggca cttaccagat gcactcgttg caggaagcgc aggatattgc gcgtagcatt
    47521 ctggaacgtg acgtacgcat caacagcaac gaagaactgg cactgccgaa agagaagttg
    47581 caggaactgc acatctagtc agtaaactat cttcacaatt aattgaaaaa agccagttca
    47641 aatgaactgg cttagttgta cttagtgcgc accgccgcca ccgccacctg cgccaaatgg
    47701 cggtttagca aaccacacca gccccagcag tacgaggaat atcccggctg acatccagaa
    47761 gatctcattg gcggaaataa tcagcccctg attggtgatc tgctgggcaa tccagcctga
    47821 tgcctgctgt tgcgtcatcc caagcccttc cagttgactg tacatcgcct gggcattcgg
    47881 gttgaacggg tttaccgact cagtcaactg cgcatggtgc atcgactcgc ggttggtcca
    47941 catggtcgtg gttatcgacg tgccgataga ccccgccagc gttcgcgtaa agttagagag
    48001 gctcgatgcc gccgccagtc gttccggtgg caaaccagac agcgtaatgg tggtcagcgg
    48061 cataaagaag caggccaccg caaacccctg gataaactgc ggccaggccg acgcgccaaa
    48121 atccatacct ggttcaaagg tataggcacg ccagtagaag cagacggcat acataataaa
    48181 gctgaaggtt accagccgcc gcatatccag tttatgcgcg aagcggccga taatcggcga
    48241 caggatcacc ggaataatcc ctaccggcgc agaggccaaa cctgcccagg tcgccgtgta
    48301 accgtagacc tcctgcaaca actgcggcag cagaacaata gcgccgaagt agagcatata
    48361 cgcgaggctg atacacaagc agccgatggt gaagttgcgc gacttaaaca acgacagatc
    48421 gactatcggg ttatcgtcgg tcagctccca gacaatcagg aagcagatag ccaccaccgc
    48481 caccacggta aggatgataa tttcctgtga tgaaaaccag tccagctctt taccgcggtc
    48541 gagcataatc tgcaggctgc cgataccaat aaccagcagt gccagcccca cggcatcaat
    48601 ccgccgccgt ttggtgcggg tttcacgtcc gcgcagagtt tgcagtgtca tcaacaccac
    48661 cgccacgcca atcggcacgt tgatgaagaa tatccagccc cagtggtaat tatcgctgat
    48721 ataaccgccg aggatcgggc cgcaaattgg cgcgacaatc accgtcatcg accacaacgc
    48781 cagcgcgatc gagcgtttgg ctggcgggta gttattcagc aatagacttt gcgaaagcgg
    48841 gatcaacggc ccggcgacaa tcccctgaat cacgcggaag aagatcagca tattcaggct
    48901 gctggagaca ccacacgccc acgacgcaat agcaaaggcg atggtggacc aaaggaacag
    48961 tttcacttcc ccgacgcgct ttgccagcca gccggtaagc gggatcgaga tggcattcgc
    49021 caccccgaaa gaagtgatta cccacgttcc ctggctgagc gatgagccca gattcccggc
    49081 gatagtgggg atcgccacgt tagcaatggt ggagtccagc acctgcatga atgtcgccag
    49141 tgacagcgca atcgtcataa tgaccagttg cgcgccttcc agcggttttt gctgttgcat
    49201 cacacgcacc tctggattag ccagcgttag cttttacgat atcgtcgatc agtttattga
    49261 caggtgccag gctgatttca cgcgcggtac ttaccgcgac cggtgttgaa cgtactttat
    49321 ttgccagtac ctgaccgtca cggttagtgg tattgacgct caccagtgtg gacaaaccaa
    49381 tgcgcagcgg gtattgctcc agctgtttct ggtccagttc gatacgcaca ggtagacgct
    49441 gaacgacttt gatccagtta ccggtcgcat tttgcgccgg aagcagtgag aatgcgctac
    49501 ctgtgcccat atccagacca accactttac cggtgtattt cacatcatcg ccgtaaatat
    49561 ccgtggtgat agtgaccggc tgaccgatac gcatattggc aatctgcgtc tctttaaagt
    49621 tggcatccac ccacatattg gtggctggaa cgaccgccat cagcggcgtc gttgggctaa
    49681 tttgcgcccc aggctgtacc gcacggcggg agacataacc ggtcatcgga ctgacaatac
    49741 gggtacgctc cagcgccagc caggcgttac gtacttcggt ggcagcctgt tgcacggcag
    49801 gctgatcttc cagtttagtc cccagaatca tcgcctgatt ggcattgtat tgttgaatag
    49861 cgacgtccag ttgcgcctgg gcactggtga cggcgtcgcg ggcgtgttgc agctcttcgc
    49921 gaccaatcag gttggcattg cccagcggca cacggcggtt gtagtcgctt tgtgcttttg
    49981 cgagggcgat tttctgcacc tcaatattcg cctgcaactg tttgctgtta atcatcagct
    50041 ggtgggtttg gcgaacgctg gaagccagtg cagttttggc tttttcaaac gcctggcgag
    50101 catctgtcgg gtcgagagtg accagcacgt cgccttcttt tacaaaatcg gtgttatcgg
    50161 cccagacttt cgtcacgctg ccagacacct gagacataat ttgaatctga ttccctgcca
    50221 cgtatgcgtc atcggtttct tcgaaatgac gcagtaccaa aaaccaataa atccctatcg
    50281 ctacggcaat aattataaag agcaaggtga gaaggaggag cagacgctta cgtttgccgc
    50341 tcttctttac cggttgctgc ggggtttgag tctccgcatt tgcgctcata ttgttctcca
    50401 cgatcttctt aactcatcgg ctgagccgac ctgcttgcca aaaaggccag catgttgcga
    50461 tgctggccgg tcattttttc ttttataaat ctggattttt gagcgagatg acgcgttagc
    50521 tcatcgcttc gagaaccaca ccgtcttgtt ccatctggtc gagacgggag agcaatttgc
    50581 gggtgatttg ctcgagctga tctttttctg ttgtgctgag cgcggaccag agttgatgca
    50641 ggcagttatg ctgcggtggt aaaacctcgc gcaaaaactc gtgacctttt tccgttaatt
    50701 gcagatgcag gcagcggcga tcgttatcgc tttcacgacg ttcgatccaa ccgcgttttt
    50761 ccagttcatc ggcaatacgc gtcgcgttgg tacgggatga tccaagagca caacttaatt
    50821 cagaaggctg aatactgtgg ttttcctgag actccagcgt aatcaacgcc ataaacaacg
    50881 tctcgttaat cccctgagcc ttcagcattt tattgcggtt ctccagcagc ttgctttgca
    50941 tgtgcatgca aagacgagtc agaaggatct cctgataagg aaaatcttcg tggcggctgg
    51001 cgcgaaattt tagcatttgt tcaatgggcg taaacgaact atccatttgg gtatgacctc
    51061 attaatttca gccgatatag taacgacagt gacaaataaa gtaaatgtat tgttttagaa
    51121 aaatgattct tgtgggttat ataatcgcca tcactttcca ggcgagccca taggccagcg
    51181 cactaagcag tgttgggatg ataatgctgc gtgttttata gaaactggca cccagtaccg
    51241 cgaagccgac cagcgtgggc acgaaacggc gtgtatcgtg catcacttct ggtgcggtag
    51301 agacaaccag cagagcgcat atcgaggcga tgccaatggt gtcgagcaaa atacctaccg
    51361 cgccacgttt ggttgggcgg gcattaccca cacgcaggcg cagcggcaaa tagcggaagc
    51421 aataattcgc cgcgccaacc agtaacccag gcagcagaac ctcatagctc atcgggcgct
    51481 ccttgccaga atgcttggat taacgcagtg aggcagccac agacaatgcc tgccagaatg
    51541 gcgacgggaa tagaaaatag cgttacgcct gcaagggcac caactaacgc tgcggtaacg
    51601 caaagagatt gtttgcgctg gaaagaggcg agcaggaaac tcataaagag tgccggaagc
    51661 ataaaaccta atgctgcttc aacggcggga taaccttgca gcaagccgct gccagagaat
    51721 gcccctatta ccgtaccaaa tacccacgat gaccatgaac tgaaggcaat gccgatcatc
    51781 cagttctcgc tccagcggcg attattgcgt accagttttg ctgttgcggc ggcaaaaacc
    51841 tcatccgtca ggccaaacgc ccacagggcg gttttcgatt tttgcagacg ctgaataata
    51901 cggctacgca gtgacgggcc atacaacaca tggcgaacat ccattgccat gacggtcagt
    51961 gcagcaaccc acaaactact cccggctgcc agcatcgcgg taatgacgaa ctggctcgcg
    52021 cctgcataaa tgatgcagga gaaaaaaacg ctttcgagag gagagaatcc cagacgggtc
    52081 gcattcagac cgaacgcaaa ggccaccgga atataactaa taacaatcgg taaactgtct
    52141 ttgcatcctt ccatgaaggt cgccgaacca ggagcaggct gtggagtagg gctttccata
    52201 gagtgtacgc ttaacgattg ttaatattat aaatagactg aatgaatatc ttaaccttat
    52261 cagactgatg ggcttcttaa cacccttata agtgtaaagc cacgaaaacg gttgctgatt
    52321 gcttaatttg cggcttcctg acgatgaaaa cctcgccacc agaccagtaa gttaactagg
    52381 gcaatcgtcg cgccagccag acaaacgcca gcccaaccgc catgttgcca ggctgaggct
    52441 gaaattagcg aaccggcggc accgccaata aagtagctgg tcatgtaacc tgcggtcagg
    52501 cgattacgcg catcagggtg tattcgataa attaccgtct ggttagtgat atgcacgccc
    52561 tgtacggtga gatccagcac caggatgccg ataatcagcg ccagtacgga agtgtgacca
    52621 aaccagatcg ccagccatga aagtaatagc agcagcagac cgaaagttgt ggtgtggtgc
    52681 gatttaccct tatcggcaaa accgcccgcc ggacgagcgc ccaacgctcc ggcagctccc
    52741 gcaagtccaa acagaccaat gacaccatcg ctgtagttaa aaggtggagc ggcaagcaaa
    52801 aaggccattg aggtccagag aatgctgaag ttggcaaagg tcaggcagcc cagcaatgcg
    52861 cgggtgcgga ggatcttatc gctgataaac atgctgaaaa cggaacctaa caactgtggg
    52921 tagttgaggt gggtttctga tttcatttgt ggcagaccac gccataatgc tagcgccatc
    52981 agtgccatta acaccgaggc aacccaaaag acggtgcgcc agccgccgag gtttgccagt
    53041 aacccggcaa cggttcgtgc cagcaagatc cccaacagca gaccgctcat aatggtgcca
    53101 accactttgc cgcgtttatc cggtgaagcc agcgtcgctg ccagcggaac cagaatttgt
    53161 gccacgactg agaataaacc ggttaatgcc gtaccgagga tcatcatcgc cagcgactga
    53221 ctgctggcag tgattaacat gccgcctgcc gccagtaagg tcatcgagac aatcaggcgg
    53281 cggcgttcaa acatatcacc gaggggaaca agaaacagca gacctgctgc atagcccaac
    53341 tgcgcggcgg taacaataaa gcctgccgaa ctggcggaaa gggaaaagtt acgcgcgatg
    53401 gtgtcgagca atggctgggc gtaatagtta ctggctaccg cctgaccggt ggcgatagac
    53461 atcagcacga tcagcgccgg gctaagctca tgattaggtt tagtcattat ttttgttcat
    53521 caggtgatga ttaagctgga aatcaataat agcggatgtt gatgaatggg ggcgaaagat
    53581 agcttgttga aatgtgcgat gtggtttgta ggcatgataa gacgcgccag cgtcgcatca
    53641 ggcattgtgc acagaacggc ggatgcggcg aaaacgcctt atccgccata cggaatgagt
    53701 gtattttgta ggcatgataa gacgcgccag cgtcgcatca ggcattgtgc acagaatggc
    53761 ggatgaggca aaaacgactt atccgcccga ataaaaatta cttctgcgct gccagcgcct
    53821 cattcaccca gccatcgaac tgctgctggt gggctttgat ccaaccatca acatggccct
    53881 gaatatcgcc ttctgaggct ttgccgtcat gcataatggc gttctgggcg ttaatatctg
    53941 ccactggcaa ctgcataatg gcaaaaagtt tcgctgctgc cgggtttttc tcggcccagg
    54001 ctttgttggc aacgatatgc atggtgctga ccgggaagcc ataattcgca ccattcggca
    54061 gtttggtatc ggcgttttta tcgcccggca gtgcggagaa cggcacctgc aaccagacca
    54121 catctttccc tggcttcagc tcattactca cccagtacgg cgtccaggtg taataaaaca
    54181 ccggtttgcc ctctttgtag cgactgatgg tgtcggccat catcgctgcg tagttcccct
    54241 gattatgcgt cacggtgttg gtcagttcat acgcggcaag ctggtggttg atcgcacctt
    54301 cgcagcccca gcctgggtta cagccggtta aatccgcttt accgtcgccg ttggtatcga
    54361 acagtttggc gatcttcgga tctttcagtt gtgcgatgtt ggtgattttg tactggtcgg
    54421 cggttttctt atcgatcagg taaccctgtg ccgcgccgtt aacgaatatc ccttcacaat
    54481 aaaatttctt atcgccaccg gcagcttcgt acatgttgtc atgcagcggc gtccagttca
    54541 cggcggtgaa ggttgcatcg ccggaagcaa gtgaggtgta gccaacgttg taatccactt
    54601 cgctgggttt attgacggta taacctaatt tctccagtgc acgactgacc agcagcgtct
    54661 ggaaggtttc ttcagtgatg gtgctctgaa ctggattaac agtaatgcct ttgcccggca
    54721 gatcggctgc aaaagtttgt gtagagataa gcgtggcaaa cgctgtcgca aaaagtacgc
    54781 tatgtcgcat tgttattcct ttttttggca gggtgataat gcccggcgtt accgggcaag
    54841 tgcagagtta cttaatgaat gggcgggtca gcagaccgac agggccagtg gtgtaccagc
    54901 gacggttgcc gcgactgcgt gagtcgcgcc caacggcctg cgtcaggcgg tcgaggataa
    54961 tggcgaggat cacaatcccg acgccgccaa cggtggcaag gcccatatcc agacgaccga
    55021 taccgcgaag taccatctga cccagcccgc cgacggcaat catcgaggcg atgaccacca
    55081 tagaaagagc cagcatcagc gtctggttaa cgcccgccat aatggtcggc atcgccagcg
    55141 gtaactgaac tttgaacagc atctggcgcg ggctggcacc gaatgagcgc gaggcttcaa
    55201 tcagatccgc cggaacctgg ttaatcccca gaatcgtcag acggataatc ggcggcagag
    55261 caaagatgat cgtcaccact acgcccggca cgttaccgat accaaacagc atgacgattg
    55321 gtaccagata aacaaacgct ggcgtggtct gcatggcatc aagcagtgga cgaataattt
    55381 tcgccgctcg cggacttctc gccagccata tccccaacgg caaaccgatg acgatacaga
    55441 acagcagggc ggttaacacc agcgccagag tcaccattgc ctgcgaccag gcaccgattg
    55501 cgccgatggc aatcagcgaa accagcgtcg ccacgcccat tccgaccccg gaaatctgcc
    55561 aggcgatgag agcgaaaacg ataatcgcca ctggcgctgg catacccagc agcaattgct
    55621 ggaaactgtt gaggatataa tcaaccggaa cgcgcacgcc ctggaagacg ggacggaaat
    55681 gggtaacaac ccagtcgatc ccttcagtga cccaactgtc gagcgggatc agcgttttat
    55741 ggaacggatc gagaatatta aaatgctcga cgtttggcgc aggcgtactg gtcagccagt
    55801 cagcaccacc gccgtcagtc ggtgcagtcg tcggtgtacc ccaggcgtct gcggattgcg
    55861 cggcactgtc cgccgctggc gtggtatccc acggattatt ttgatcagcc attatttacc
    55921 ccctcacgat ctaaagcgcg cagcagcatt cctttcgaaa tgatgccgac atactgttgg
    55981 tcctcgtcga ccacgggcac cgcacagggt gcctgtccga catgagagag caactcgcta
    56041 agaggcgttt gtgcatcgac tgctaacggc gcatcaatca gcgccgcatc aagaccttgc
    56101 tgctgcgtta acgcggtttt aagcgaatcg atggagactg cgccgacaaa cttattaccg
    56161 cgttcgataa cgtagccata ttcgcgatct tcatcctgca ataatttcag tgccgaacgt
    56221 gggccgaagc caggggtttt acgaattaag ccattcggtg tccggcgggc aatatctttc
    56281 gcactgaata cctgactaat atcaacgcca cggaagaagg tacggacata atcattcgcc
    56341 ggattattga gaatttcatc cggtgtgccg acctgtacca cttcaccatt ttgcataatg
    56401 gcaattcggt cgccaatacg catggcttca tcgagatcgt gggaaataaa gacaatggtg
    56461 cgctgatgtt tcgcctgtaa ttttaccagc tcatcctgca tctcggtgcg aattaatgga
    56521 tcgagcgccg agaaggcttc gtccattaat aaaatatccg gattaatcgc taacgcgcgg
    56581 gctaatccca cacgttgacg catcccgcca gagagttcat ccgggtagct gtgggcataa
    56641 ttttccagcc cgacctgacg cagtgcatca agggcttttt cccggcgttc ttcggcatta
    56701 attccggcca attccatacc gaacgcagta ttgtccagca cggtcatatg cggcattaag
    56761 gcaaaggact ggaagaccat cgcaatcttt tttctgcgca cctcacggag tttggcatca
    56821 gatattttgg caatatccac accatcaatc agcacttgcc cgcgggtggg ttcaatcagg
    56881 cgattgagaa ggcgtaccat tgtggattta cccgagccgg ataatcccat gatgacaaat
    56941 atctcgcctt cttcaatggc cagactggcg tcttttacgc caagcgatag cccagttttt
    57001 tccagaattt gttcttttga aagtccttgt tcgatatatt tgaatgctcg ctgtggatgc
    57061 tcaccaaata ttttataaag gtttttaatt tctaatttaa ttgccatgca atagaaagat
    57121 tcctgttatt tgtttatgtc gatatattac ccaatggaaa tagttacttt tttctaccct
    57181 aacatactga gaatctgagg caacccctga tggcaaatgt ggcatgaata ttgatgcgag
    57241 caaatcgccg gaatttcccg tgatataagg gctgagagca aatcgagaaa agttgatatt
    57301 ttttgtcaaa gatagcgaaa aaacgcctga atattgtggt ttcaggcgaa taatgcaaca
    57361 gagtatcagt gtagatcacc acaaaatatt tgcgtgataa aaagctattt ggcgggaatt
    57421 atttccctgt cgcgggtagt gatatttttg aaaataacac cctcagaaat tccagtcttc
    57481 atcttctgtt tcaaccgctt tccccatcac ataagaggag cctgaaccgg aaaaaaaatc
    57541 gtgattttca tcggcattcg gcgaaagcgc ggcgaggatt gccggattca cttccgccat
    57601 ttctgcggga aataaaggtt cgtagcccag attcatcaaa gccttattgg cgttgtaaca
    57661 gagaaacgct ttcacatcgt cagcccacgg ggtttcggcg tacagctcat cggtgtattg
    57721 cagctcgttg tcgtagagtt ccagcaacaa atcgaaggcg aaactcttca actcttcacg
    57781 ttgtcccaga gatatctttt ccatgttttt ctgatattta tagcctatgt agtaaccgtg
    57841 gactgcttca tcacggataa tcagacggat caggtctgcg gtattggtca gctttccgcg
    57901 gctggaaaaa tacatcggca gccagaaacc ggaatagaac aaaaaagatt caagaaacac
    57961 actggcgatt ttctttttca gcggatcatc accgcgataa tgttgctgaa taatctgagc
    58021 ttttcgctgc aacggtgcgt tttcttcact ccaggcgtag gcggcatcga catctttggt
    58081 ctggcacagc gtcgagaaaa tcgaactgta agagcgggca tgaaccgctt ccataaagct
    58141 gatattcgat aataccgctt cttcatgagg cgtgagtgca tcgggcatca gcgaaggcgc
    58201 gccgataaca ttttgcagcg tgtcgagcag cgtcaggcca gtaaaaacgc gcatcgtcag
    58261 ttgttgttct acgacagtta atgtctgcca ggcaggaata tcgttcgaca gcggcacctt
    58321 ttctggtagc cagaaattgc tggtcaggcg attccacacc tccagatctt tatcgtcaga
    58381 tatcttgttc cagttgatgg cgctgatacg tgagagtttc atagatattc cttaaagtgc
    58441 acaggagacg cagccttcaa tttcagtgcc ttccagcgcc atctgacgca ggcgaatgta
    58501 atagagcgtt ttgatacctt tgcgccaggc gtaaatctgc gctttgttga tatcgcgagt
    58561 ggtggcggta tcggggaaaa acagcgtcag cgacaaaccc tgatcgacat ggcgagtcgc
    58621 ttccgcgtag gtgtcgatga tcttttctgc gccgatttcg taagcatcct gatacagcgc
    58681 cagattttcg ttagtcataa acggggcagg gtagtaaacg cgtcctgttt tgccctcttt
    58741 gcgtatctct actttcgcca caatcggatg aatactcgac gtagcatggt tgatataaga
    58801 gatagaaccg gttggcggca ccgcctgaag attctggttg taaatgccgt agcgcatcac
    58861 gtcgtcgcgc agttgcgccc acatctcacg ggtaggtaac gtaataccgc tgcgggcaaa
    58921 cagttcgcca actttcgccg ttttcggctg ccagttacct tgcagatatt ggctaaaata
    58981 ttcaccactg gcataacgtg actgtttgaa cccggcgaag gtttcaccgc gttcgcgcgc
    59041 cagcaacatc gaggtacgca gtgcgtgcca ggtgatggca tagaaataga gattggtgaa
    59101 atccagtgct tccggcgaac cataagcgat gccttctcgc gccagatagc cgtgtaaatt
    59161 catctgcccc agtccgatgg cgtgtgaggc ggcatttccg gcttcgatgg acggcacgct
    59221 gcggatatga ctcatatctg acaccgccgt taaaccgcgc acggcagtct ctaccgtgcg
    59281 ggcaaaatcg ggggaatcca tggtgtgcgc aatattcaac gaacctaaat tgcaggagat
    59341 atcatggcct gtgcgggcat agtcgagatt ctcgtcatac tctgaggcac agttgacctg
    59401 caaaatctct gagcagagat tactcatatt tatgcgcccg gcgacagggt tagcacggtt
    59461 taccgtgtct tcatacatga tgtagggata gccggactca aactggattt ctgccagccg
    59521 ctggaagaaa tcacgggcgt tgaggtattt tttgcggatg cgttcatcgg caaccagttc
    59581 gtcatagtgt tcgctgatgg cgatatcggc aaacggcttg ccataaactc gctcaacgtc
    59641 atagggcgaa aacagcgcca tctgcgcatt ctcttttgcc agatggaaag tgatatccgg
    59701 gatcaccacg cccagcgaca gtgttttaat gcggattttt tcgtcggcat tttcccgttt
    59761 tgtatcgaga aaacgcagaa tatcgggatg atgagcatgt aaatagactg cacctgcccc
    59821 ctgacgcgcg ccgagttggt tggcatagga aaatgcgtct tccagcattt tcatcaccgg
    59881 aattacgcca gaagattgat tttcaatacg tttaattggc gcgcccgctt ctcgcagatt
    59941 cgacagcaaa aatgccacgc cgccgccgcg tttcgacagt tgcagtgcgg aatttaccgc
    60001 ccggccaatc gactccatat tgtcttcaat acgcagcaga aaacaggaaa ccagttcgcc
    60061 gcgctgctgc ttaccgcagt tgaggaatgt cggcgtggct ggctgaaagc gtcctgacag
    60121 catttcatcg gtcagttgca acgccagcgt cgcatcgcct tgtgccagcg tcagcgccac
    60181 catcgtgact cgatcggcaa aatcttccag ataacgttta ccgtcgaatg tcttcaacgt
    60241 atagctggtg taaaacttcc atgccccgag gaatgtctgg aaacgaaaac cgctggtgtg
    60301 tgcgtgggca aacagcgtaa tgacaaagtc gcgagagtag cgattaagaa cgctttcatc
    60361 atagtaacct tcgttgacca gccagttcag gcgctgctgc tggctactga aggtcacact
    60421 gttcggacgc acatgcgtcg ccataaaggc gtcaacggcc tggcggtctt tatcgaactg
    60481 aatgcgacct gcgctatcgt agaggttaag catcgcattc agcgcgtggt aatccatcgt
    60541 ttcctgcgtc aggcattctg cggttgtcgt tgccaaaatt cggttactcc tttacgaacg
    60601 ttttcgatat cgctttgcgt acccatgagt tcaaaacggt acagccacgg cacgccgcat
    60661 ttccgggcaa tcacatctcc ggcgcggcca tacgcctcac caaagttgcg attaccagaa
    60721 gcaataacgc cgcgaagcaa cgcccggttg tgctcgtcgt ttaaaaagcg aattacctat
    60781 cgtggcaccg cgccagccgt accgccgccg ccgtaagagg gcacgatcag gatgtaaggc
    60841 tcgtctacct gaatccgttc ccgctcattg agcgggatgc gcaccgcggg cagacctaaa
    60901 cgttcgataa aacgctgcgt gttttcggag ctgctggaga agtagacgag ctggctcatg
    60961 cactggccgc gtgtggcgct ggatgcagac ggttaatcat gtccggacgg aaaccagacc
    61021 agctaagatc gccagcaatc actaccggca actgacgaaa gccctgagca cgcaacgctt
    61081 ccgctgcttc aggaacgcga tcgacattaa tcatttcaaa atcaaagccc cggttttcca
    61141 tcgcccgttt ggtggcgtgg cactgaacgc aatcgttacg agtgtaaata gttatgcgca
    61201 tgattcgtat ttccgtttaa aatgaagata cggcgcgatg atacgcgtcg ggttgtctct
    61261 ctgttgatac agagatacta gatgtagttg aaaaaagatt caaccacaca atatatagca
    61321 aatagtgatc gaaattaccc tagatatgag cgtgacgggg gagggggatt tttgtgattc
    61381 accaggcaaa aagaaacccc gaagacaggc ttcggggtta aagacgcgta tttattatca
    61441 tttttgcact acgatttgcg catgcttaac agtgcgccga taaaaatacc taccgcagct
    61501 gctgtaccca cgctacacca gggtctttca cgaacaaaag aatccgcgca gccaacggca
    61561 tcgcgtgcgg cttgctggac gcgagtacga ccatgcattc gcgccctggt ttctttcagc
    61621 aatgcctgag ctttactgcg tgcggcttca gcttcccctt tggcgtcgct gccccaggac
    61681 ttcaatacag attcgaggct gtcagctaat tgattgacat cattctggat atcctgcacg
    61741 ccatcatcaa cgtcgttgcg gttcggtcgg ttaaacatat gatcccccat tagaattcga
    61801 tgtaattttc agtgtagacc ataattttct taactgaggt tcatcgcggc tttatggact
    61861 tttctgaaag cgttgtgaat gcgcaatcag gtaaggtagg cccgcggttg ataataacga
    61921 ggatcaaata tgtatttacg accagacgag gtggcgcgcg tacttgaaaa agtcggtttt
    61981 actgtcgatg tggtaaccca gaaagcttat ggttaccgcc gtggggaaaa ttatgtctat
    62041 gttaatcgcg aagcgcgaat gggacgtacc gcactggtaa ttcatccaac attaaaagaa
    62101 cgtagttcga cactggctga acccgcttcc gatattaaaa cttgcgatca ttatcaacaa
    62161 tttccgctct atttagcagg cgagcgacac gagcattacg gtatcccgca tggcttcagt
    62221 tcgcgtgttg cgcttgaacg ttatttgaat ggtttatttg gcgaagccag ttaaagacgc
    62281 gactggcgat gccagtcgcg aaaagaagag tcaggctttt acctgctggt aatggctgac
    62341 tttaaacagt cggcggcaat aatcgaggaa gtagccataa accgccccca tcaacatcga
    62401 aacaacgatg tttgaactga ccgccgccat aatctgatgc cagtctgcgc ccaccactaa
    62461 caagatcgcc acatacaccg gtgactggaa cgtcacataa gccaggatat cagccagatt
    62521 ttttatccag cccgacgggc taactttgcg tgccgcgcgc ataaatagat cgcggtacat
    62581 accgtatggc catgcaatta agatgttcac cggaatcgct accaatctgg aataaaaaga
    62641 ctgttcgaag ctcattccgg agaggaaaac ttcaatacac atgttcacga cagaacagta
    62701 aacaaccatc gcgaacgtat ctgcaactgc atgacgcaag cgtgactgcg gtgagaacat
    62761 gctaatgctc cttaataaaa gcgtaatggg atgttaatgg agatgaaagc agtggatctc
    62821 attgccatct gcattttcgc atagcgtata tcgctggcta tgactattca attagtgaaa
    62881 aatttttatt tagtcagttt ttgtagataa aatattccgt aacggctcgt tttttgtaca
    62941 ttgtggattt ttacatgctg atatgattta ttttcaatta aatcaattag atatgacctt
    63001 gcctttaatt tcactaatga aagtttgggg gtgaggtaac gctataagcg tagagaattc
    63061 ggtgtatgtc gtgaccgtgt catttacagc atttttgatg ccagaaaatg tacattaaca
    63121 gcagaaagca ccagtg
//
LOCUS       DAEEKQ010000008        60958 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.008, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000008 DAEEKQ010000000
VERSION     DAEEKQ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.008"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..468
                     /locus_tag="KLK57_000505"
     CDS             <1..468
                     /locus_tag="KLK57_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0890974.1"
                     /translation="SGTSVTGSTVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIET
                     KLVSTKVGTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYD
                     PRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            581..1375
                     /gene="panB"
                     /locus_tag="KLK57_000506"
     CDS             581..1375
                     /gene="panB"
                     /locus_tag="KLK57_000506"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HBE0890975.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTASVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPIELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            1387..2238
                     /gene="panC"
                     /locus_tag="KLK57_000507"
     CDS             1387..2238
                     /gene="panC"
                     /locus_tag="KLK57_000507"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HBE0890976.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIIAIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKIVELA"
     gene            complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="KLK57_000508"
     CDS             complement(2530..3426)
                     /gene="rpnC"
                     /locus_tag="KLK57_000508"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HBE0890977.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMVFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFAQRFLSKG
                     MSREDVAEMTNLSLAEIDRLIN"
     gene            3700..4080
                     /gene="panD"
                     /locus_tag="KLK57_000509"
     CDS             3700..4080
                     /gene="panD"
                     /locus_tag="KLK57_000509"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HBE0890978.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(4084..5313)
                     /locus_tag="KLK57_000510"
     CDS             complement(4084..5313)
                     /locus_tag="KLK57_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HBE0890979.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLVTWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQVRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLVSNQPQG"
     gene            complement(5377..5817)
                     /locus_tag="KLK57_000511"
     CDS             complement(5377..5817)
                     /locus_tag="KLK57_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HBE0890980.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5922..6692)
                     /locus_tag="KLK57_000512"
     CDS             complement(5922..6692)
                     /locus_tag="KLK57_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0890981.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(6689..7615)
                     /locus_tag="KLK57_000513"
     CDS             complement(6689..7615)
                     /locus_tag="KLK57_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0890982.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7724..8386
                     /gene="can"
                     /locus_tag="KLK57_000514"
     CDS             7724..8386
                     /gene="can"
                     /locus_tag="KLK57_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HBE0890983.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="KLK57_000515"
     CDS             complement(8427..8963)
                     /gene="hpt"
                     /locus_tag="KLK57_000515"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HBE0890984.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILRDE"
     gene            9169..11559
                     /gene="gcd"
                     /locus_tag="KLK57_000516"
     CDS             9169..11559
                     /gene="gcd"
                     /locus_tag="KLK57_000516"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HBE0890985.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWCSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGSILTWAGFNDPQEINGTLSADATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKINEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="KLK57_000517"
     CDS             complement(11606..13156)
                     /gene="cueO"
                     /locus_tag="KLK57_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HBE0890986.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRTVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGM
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            13322..13669
                     /locus_tag="KLK57_000518"
     CDS             13322..13669
                     /locus_tag="KLK57_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HBE0890987.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13775..14641
                     /gene="speE"
                     /locus_tag="KLK57_000519"
     CDS             13775..14641
                     /gene="speE"
                     /locus_tag="KLK57_000519"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HBE0890988.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGIVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSYRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            14657..15451
                     /gene="speD"
                     /locus_tag="KLK57_000520"
     CDS             14657..15451
                     /gene="speD"
                     /locus_tag="KLK57_000520"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HBE0890989.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(15489..15851)
                     /locus_tag="KLK57_000521"
     CDS             complement(15489..15851)
                     /locus_tag="KLK57_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HBE0890990.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="KLK57_000522"
     CDS             complement(16026..18623)
                     /gene="acnB"
                     /locus_tag="KLK57_000522"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HBE0890991.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18978..20735
                     /locus_tag="KLK57_000523"
     CDS             18978..20735
                     /locus_tag="KLK57_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HBE0890992.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASAGLYIKSRTVEAPVETQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVKQTVTAPVIPSN
                     TVLTANPVITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FVDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRIQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="KLK57_000524"
     CDS             complement(20806..22230)
                     /gene="lpdA"
                     /locus_tag="KLK57_000524"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HBE0890993.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="KLK57_000525"
     CDS             complement(22555..24447)
                     /gene="aceF"
                     /locus_tag="KLK57_000525"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HBE0890994.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPASAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKLTPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="KLK57_000526"
     CDS             complement(24462..27125)
                     /gene="aceE"
                     /locus_tag="KLK57_000526"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HBE0890995.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYIPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="KLK57_000527"
     CDS             complement(27286..28050)
                     /gene="pdhR"
                     /locus_tag="KLK57_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HBE0890996.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            28591..29964
                     /gene="aroP"
                     /locus_tag="KLK57_000528"
     CDS             28591..29964
                     /gene="aroP"
                     /locus_tag="KLK57_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HBE0890997.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAIVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="KLK57_000529"
     CDS             complement(30007..30861)
                     /gene="ampE"
                     /locus_tag="KLK57_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HBE0890998.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="KLK57_000530"
     CDS             complement(30858..31409)
                     /gene="ampD"
                     /locus_tag="KLK57_000530"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HBE0890999.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            31497..32390
                     /gene="nadC"
                     /locus_tag="KLK57_000531"
     CDS             31497..32390
                     /gene="nadC"
                     /locus_tag="KLK57_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HBE0891000.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVSTRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            32593..33033
                     /gene="ppdD"
                     /locus_tag="KLK57_000532"
     CDS             32593..33033
                     /gene="ppdD"
                     /locus_tag="KLK57_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HBE0891001.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            33043..34429
                     /gene="gspE"
                     /locus_tag="KLK57_000533"
                     /pseudo
     CDS             33043..34429
                     /gene="gspE"
                     /locus_tag="KLK57_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
     gene            34419..35621
                     /gene="hofC"
                     /locus_tag="KLK57_000534"
                     /pseudo
     CDS             34419..35621
                     /gene="hofC"
                     /locus_tag="KLK57_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414648.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
     gene            complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="KLK57_000535"
     CDS             complement(35656..36699)
                     /gene="guaC"
                     /locus_tag="KLK57_000535"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HBE0891002.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(36855..36899)
                     /locus_tag="KLK57_000536"
     CDS             complement(36855..36899)
                     /locus_tag="KLK57_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891003.1"
                     /translation="MIIKTLKMSARKRN"
     gene            36924..37544
                     /gene="coaE"
                     /locus_tag="KLK57_000537"
     CDS             36924..37544
                     /gene="coaE"
                     /locus_tag="KLK57_000537"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HBE0891004.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            37544..38287
                     /gene="zapD"
                     /locus_tag="KLK57_000538"
     CDS             37544..38287
                     /gene="zapD"
                     /locus_tag="KLK57_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HBE0891005.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            38297..38494
                     /gene="yacG"
                     /locus_tag="KLK57_000539"
     CDS             38297..38494
                     /gene="yacG"
                     /locus_tag="KLK57_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HBE0891006.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="KLK57_000540"
     CDS             complement(38594..38983)
                     /gene="mutT"
                     /locus_tag="KLK57_000540"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HBE0891007.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(39043..41748)
                     /gene="secA"
                     /locus_tag="KLK57_000541"
     CDS             complement(39043..41748)
                     /gene="secA"
                     /locus_tag="KLK57_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HBE0891008.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(41810..42322)
                     /gene="secM"
                     /locus_tag="KLK57_000542"
     CDS             complement(41810..42322)
                     /gene="secM"
                     /locus_tag="KLK57_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HBE0891009.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="KLK57_000543"
     CDS             complement(42553..43470)
                     /gene="lpxC"
                     /locus_tag="KLK57_000543"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HBE0891010.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="KLK57_000544"
     CDS             complement(43571..44722)
                     /gene="ftsZ"
                     /locus_tag="KLK57_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HBE0891011.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="KLK57_000545"
     CDS             complement(44783..46045)
                     /gene="ftsA"
                     /locus_tag="KLK57_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HBE0891012.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="KLK57_000546"
     CDS             complement(46042..46872)
                     /gene="ftsQ"
                     /locus_tag="KLK57_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HBE0891013.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="KLK57_000547"
     CDS             complement(46874..47794)
                     /gene="ddlB"
                     /locus_tag="KLK57_000547"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBE0891014.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(47787..49262)
                     /gene="murC"
                     /locus_tag="KLK57_000548"
     CDS             complement(47787..49262)
                     /gene="murC"
                     /locus_tag="KLK57_000548"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HBE0891015.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLIGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(49316..50383)
                     /gene="murG"
                     /locus_tag="KLK57_000549"
     CDS             complement(49316..50383)
                     /gene="murG"
                     /locus_tag="KLK57_000549"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HBE0891016.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="KLK57_000550"
     CDS             complement(50380..51624)
                     /gene="ftsW"
                     /locus_tag="KLK57_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HBE0891017.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(51624..52940)
                     /gene="murD"
                     /locus_tag="KLK57_000551"
     CDS             complement(51624..52940)
                     /gene="murD"
                     /locus_tag="KLK57_000551"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HBE0891018.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIAQAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQLGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="KLK57_000552"
     CDS             complement(52943..54025)
                     /gene="mraY"
                     /locus_tag="KLK57_000552"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HBE0891019.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(54019..55377)
                     /gene="murF"
                     /locus_tag="KLK57_000553"
     CDS             complement(54019..55377)
                     /gene="murF"
                     /locus_tag="KLK57_000553"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HBE0891020.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(55374..56867)
                     /gene="murE"
                     /locus_tag="KLK57_000554"
     CDS             complement(55374..56867)
                     /gene="murE"
                     /locus_tag="KLK57_000554"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HBE0891021.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKAKDEATDGEIREMHGVPVIYLSQLNERLSALAGR
                     FYHEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENT
                     TGSAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHG
                     DMEHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWL
                     KAIDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKT
                     AARLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDR
                     DKGKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAV
                     TCAVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="KLK57_000555"
     CDS             complement(56854..58620)
                     /gene="ftsI"
                     /locus_tag="KLK57_000555"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HBE0891022.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPGSIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="KLK57_000556"
     CDS             complement(58636..59001)
                     /gene="ftsL"
                     /locus_tag="KLK57_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HBE0891023.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="KLK57_000557"
     CDS             complement(58998..59939)
                     /gene="rsmH"
                     /locus_tag="KLK57_000557"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HBE0891024.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAVATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="KLK57_000558"
     CDS             complement(59941..60399)
                     /gene="mraZ"
                     /locus_tag="KLK57_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HBE0891025.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAVGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14595 a        16854 c        14998 g        14511 t
ORIGIN      
        1 agtggaactt ctgtgactgg ttctacggta aaaatgggtg aatatagtgc agaacaaatc
       61 agaaacggtg ccacaccggt tccttttgat atttcacttc aaaactgcgt tcgtgtgact
      121 aatattgaga caaaattagt ttcaacaaag gttggtactg aaaacgggca actccttggt
      181 aatactctca caggtaatga cgcagcaaaa ggagtcggtg tactcataga aggtttagca
      241 actagtaaaa atcctctaat gacattgaaa cctaatgatt caaattctgt ttataaagat
      301 tacgacccga gaggcaaaga cgatacaaca ggaggagttt acccggatca agatacaggt
      361 ataacatacc ctctccattt ccaggccacg ctacaacagg atggaactat accaatagaa
      421 gctggtgagt ttaaagccac cagtactttc caggtaacct acccttaata agtccaccgc
      481 accgccatca tctggcggtg cggtaattga taaatcattg cccccaccaa tgtgactgat
      541 aaaatgccag gcaacacgac atcatttatc aggacacgtt atgaaaccga ccaccattgc
      601 ttcacttcag aaatgtaagc aggataaaaa gcgtttcgcg accatcaccg cttacgacta
      661 cagcttcgcg aaactatttg ctgatgaagg acttaacgtc atgctggtag gcgactcgct
      721 gggcatgacg gttcagggtc acgactccac cctgccggtg accgttgccg atatcgccta
      781 ccacactgcc tccgtacgtc gtggtgcgcc aaactgcctg ctgttggctg acctgccgtt
      841 tatggcgtat gccacgccgg aacaagcctt tgaaaacgcc gcaacggtta tgcgtgccgg
      901 tgccaatatg gtcaaaattg agggcggtga gtggctggtc gaaaccgtaa aaatgctgac
      961 cgaacgtgcc gttcctgtat gtggtcactt aggtttaacc ccacagtcag tgaatatttt
     1021 cggtggctac aaagttcagg ggcgcggcga tgaagcgggc gatcaactgc tcagcgatgc
     1081 attagcctta gaagccgctg gggcacagct gctggtgctg gaatgcgtgc cgattgaact
     1141 ggcaaaacgt attaccgaag cactggcgat cccggttatt ggcattggcg caggcaacgt
     1201 cactgacggg cagatcctcg tgatgcacga cgccttcggc attaccggcg gtcacattcc
     1261 taaattcgct aaaaatttcc tcgccgaaac gggcgacatc cgcgcggctg tgcggcagta
     1321 tatggctgaa gtggagtccg gcgtttatcc gggcgaagaa cacagtttcc attaaggagt
     1381 cacgttgtgt taattatcga aaccctgccg ctgctgcgtc agcaaattcg ccgcctgcgt
     1441 atggaaggca agcgcgtggc gctggtgcct actatgggta atctgcacga tggccatatg
     1501 aagctggtcg acgaagccaa agcccgcgcc gatgtggtcg tcgtcagtat tttcgttaac
     1561 ccgatgcagt tcgaccgccc ggaagatctg gcacgttatc cacggacctt gcaggaggac
     1621 tgcgagaagc taaacaaacg taaagtggat ttagttttcg ccccttcggt aaaagagatc
     1681 tacccgaacg gtactgaaac ccatacttac gttgacgttc ccgggctttc gaccatgctg
     1741 gaaggcgcga gccgtccggg gcattttcgc ggcgtttcga ctatcgtcag caagctgttt
     1801 aacctggtcc agccggacat cgcctgcttc ggtgaaaagg actttcagca actggcgcta
     1861 atccgcaaaa tggttgccga tatgggcttc gatattgaaa ttgtcggcgt cccgattatg
     1921 cgcgccaaag atggccttgc gctcagttcc cgtaatggtt atctgacggc ggaacaacgc
     1981 aaaattgcgc ccggtctgta caaagtttta agttcaattg ccgacaaatt gcaggctggc
     2041 gagcgggatc ttgatgaaat tattgccatt gcagggcaag aactgaatga aaaaggcttc
     2101 cgcgccgatg atattcagat tcgcgatgcc gacacgttgc tggaagtctc tgaaaccagc
     2161 aaacgagctg taattctggt agccgcctgg cttggcgatg cccgcctgat cgacaacaaa
     2221 atcgtcgaac tggcgtaatg cttttactct aaataattca agttgcagga aggcggcaag
     2281 cgagtgacaa attcgtcagg aacgaatttg cccagccgca ggctggcctt cggtgagaga
     2341 catggatgtc tctcattaat ccccaggagc ttacataagt aagtgactgg ggttagcgaa
     2401 cgcagacgca gcacatgcaa tttaaagtat gacgagtata actggcgcta cggctgatgg
     2461 cgccaattat taatttgccc cacgtcatgg cgatggcgtc tgtaagcaat tattcataac
     2521 gcagacaact tagttaatca gcctatcaat ctcagcaaga gataaatttg tcatctctgc
     2581 aacgtcttcc cgagacatcc ctttactcag aaaacgctgg gcgaattctt gcctttcctc
     2641 ctgtcttccc tgctgaatcc ccttctcaat ccctttctct tcaaaccact gcgccagcgt
     2701 catcatagac tcccctcccg tttccctgtc tctcagcaca ccataaaaca aatccgcttg
     2761 ttcagtatga ccgcgttgca gcatatagtt ttgcatggca actaactgac ttccgctagt
     2821 gtacccttcg tcaatcagcg tgaccagttg ctccagcaat aacattaagt cgcgctggcg
     2881 aatatgtttt tgcagtagtt cgagaatcgc aatccgccga tgttgcatga tttcgtcatc
     2941 cggtgtgatg gtgatatcca ccagcgggaa aggactgtta tagacgcgtc gtgccagctc
     3001 cggcgagtaa aatatatcaa accagcacat tgagagcgga taaggtgtgg cctcgccctg
     3061 ataaaacagt atcggcacca ccagcggcag cttatcgtga tccgcctcca gatgccggtg
     3121 catggcagca atagaataac gcatcatgcg aaagaccatt ttcttatccg gcttgctttg
     3181 gtgttcaatc acaacatgca gataaccggg attgccctgc atttgcacgg aatagagcac
     3241 gtccgtgctg tgtcctttca ggctctcttc aatgaaactc cccgactcta aatgaagcgt
     3301 gttgaggtca caaagttctc gtaattccac tggcaaatgt atctccagaa agtcgcgagc
     3361 cgtctccgcg tgcattaaaa attgtttaaa cactgcatca tgcggtgtgg tactcggtgc
     3421 atccatcttc cctgccttcc gtgttgtctt gcgtgaaggc agtatccagt caaggcgtgc
     3481 catcgtcagc cacctcttta gtgtttaccg agcagcgttc agagaaattt caattggtta
     3541 cagccgttac atggcacgct tcggcacggt acgcaaactg ctgatgtaaa tgccgtcaga
     3601 tgagtagaca ctaaacaaaa atcgggcaat actgcgtgag aatttcctca aagcaggcat
     3661 cgcctgcttc gttaacgaca gggtagaaag gtagaagtta tgattcgcac gatgctgcag
     3721 ggcaaactcc accgcgtgaa agtgactcat gcggacctgc actatgaagg ttcttgcgcc
     3781 attgaccagg attttcttga cgcagccggt attctcgaaa acgaagccat tgatatctgg
     3841 aatgtcacca acggcaagcg tttctccact tatgccatcg cggcagaacg cggttcgaga
     3901 attatttctg ttaacggtgc ggcggcccac tgcgccagtg tcggcgatat tgtcatcatc
     3961 gccagcttcg ttaccatgcc agatgaagaa gctcgcacct ggcgacccaa cgtcgcctat
     4021 tttgaaggcg acaatgaaat gaaacgtacc gcgaaagcga ttccggtaca ggttgcttga
     4081 ttgttatccc tgcggctggt tactcaccag ccgcgacatc gtctccagcg aatccgttct
     4141 taagatataa agtcgtttta gtaacaacgg attatccccc ggttttactt tgcctttcat
     4201 ggttgtcacc gccaggtgaa atccggcatc gtttgctgcc ttcacggctt tgtcattaaa
     4261 tccgccaaac ggatacgaaa gataccagac atgcggatta aattgcgcca gagcgcggcg
     4321 tgaacgtgca aaatcaaaca gaatattgtg ctcactacgg ctcagtaata tgggtcggcg
     4381 atagccatcc acccgatgca aaaaatgggt atgtgactga aaatcaaaaa catcgcgaat
     4441 ttcgttaagc tcagaaacgc tcataaattg cagcgatttt gggttccact tctgcgggtg
     4501 acgtttgatg cgcgaggtaa caacaaacgc cgtcgccttc atgccatatt gtttcaatac
     4561 aggatacgca tagcggctca ccgacttgag gccatcatca aaggtaatca ccaccgctcg
     4621 cgcagggaga ttgatcttat tcttcacgta accttccagc tgcgccatgc tcagtgtcgc
     4681 gtatcccctg tcgcgcagcc aggccatttg gttattgaaa gcgcgtaccg aggtggtcgt
     4741 cgaagtatgg cgaaaacggg tgttttcttc gtcgcgcaga atatggtgat aagttagcac
     4801 cggaaggcca ttatcgggtt gggcatccag cgcgctgata taggccagtc gatcgccaat
     4861 acggacctga taccaggttt gatttaacct gtctttcagt ttatgcaaaa tcgggtagcg
     4921 caaattgtcc gccaataccc caaatggcgc actgcccaca ctcggcgcgt tatagaccgg
     4981 cgtatctttc caggtaacta agttctgatt actcagcggc ttgttgaggt cacccaaacc
     5041 gtcttcaact ttttgtcgcc cctgaaccgg ctcgagatgc cctttatcga taaacccttt
     5101 accaaagcca aaattaaatg cgtaataact tgcggcagtg ggctccacgg caatgatttg
     5161 tccggcccga atattcccca cggtcaccat cttgtcgcca atttgcgccc agaccgcagc
     5221 attttcgatg gtttgcatat aacgggcagg taacgcggca ctgacacttg cggtaaacag
     5281 catcagcagg agaataacag cttgtttgta catgaaaaaa taaccgaggg tgaaacaacg
     5341 cttttattgt agcaaaagcg ctataagaat cgtgggttaa tactcataca aattatgttt
     5401 gaggacgaaa ggcggatttt tttgttgttg gtgccaaaga ctactcacct ccggcccacc
     5461 cagttcgaca atacgctcgc gaaactctga actggtcatg gtttcacggc aagccatcat
     5521 ctgttcaatt aacggtaacg tgacaccaga aatcacttcg caacgggagt gtttgtggct
     5581 taataatgaa gccacgcggt acggtggcgc tccggctata tcggttaaga agatgacacc
     5641 ctcaccagag tccgcttcat gcagagcatc gcacatcatg cggctgagca tattagagct
     5701 taacccacgc cagaaattca cggcccggca ctgaagaagc gccccatgtt ttttctccag
     5761 cgcatccagt atctcttgcg cccggtcatc gtgacaggta attacccaac ctaacatcgc
     5821 cttcctcctt agccgaaaat cagtctaccc tgaccattgt tgataaaggg tgatagccgt
     5881 caaaactcct ctcccccaaa tccagaggag aggaaatagc cttagctacg caaaccacgt
     5941 ccacgttgga tcagcgacca acagatcaaa taaaacgcca caataaagac caccagtacg
     6001 ccaaaggtag tgaccagcgg aacatcattg ataccgagga agccgtagcg gaaaccactg
     6061 atcatataaa cgattgggtt caggtgcgac agcccttgcc agaacggcgg caacaaagtc
     6121 agtgagtaaa agaccccacc caaatacgtg agtggcgtta acacaaaggt tggcaccagg
     6181 ctgatgtcat cgaacgtttt ggcaaacaca ccgttcagca aacccgcaag ggagaacaac
     6241 accgccgtga gcaccagggt taaggcaacg aatacccacg aatgcacctg aaacggcaca
     6301 aaaaacagtg aaattgccgt caccagaatg ccaacaaaca gaccacgcgc cacgccaccg
     6361 ccgacatatc cggcaataat gacgtgagtc ggaaccggcg ctaccagcag ctcttcaata
     6421 ttacgctgga acttggcacc aaaaaatgat gacgcaacgt tggcgtaggc attggtgatc
     6481 accgacatca tgatcagccc cggtacgatg aactgcatat agctgaagcc atgcatatcg
     6541 ccaatacgcg aaccaatcag gttaccgaag ataataaagt aaagggtcat ggtgatgact
     6601 ggcggcacca gcgtctgcac ccagatacgc ataaagcgat ggatctcttt cgcccagatg
     6661 ctttttagcg ccacccagta aagatgcatc atgcgcgatc tccttgtttt tcattaacca
     6721 gtgaaacaaa cagctcttcc agacggttag ctttgttacg catacttaat acctgaatgc
     6781 cctgctcact taactgcgta aatacgctgt tgatcccctg ctcacgcagc acttcaactt
     6841 ccagcgtcgc ggtatcgacc agtcgatact gatagccatc gagcttcggt aacggacttt
     6901 tcggtgcgag atcgagaata aaggtttccg atttcagctt cgccagcagc gccttcatcg
     6961 aggtattttc caccagctca ccgtgttgaa taatgccgat attgcggcac agcatttctg
     7021 cttcttccag gtagtgtgtg gtgagaatga tggtggtgcc tttgtcgttt aaatccttca
     7081 aaaagcccca cattgagcgg cgaagttcaa tatccacgcc tgcggtcggt tcgtcgagaa
     7141 tcagtagttt aggttcatgc attaacgcac gggcaatcat taaacggcgc ttcatcccgc
     7201 cagataacat acgcgcacgt tcgttgcgtt ttccccatag atcgagttgt ttaagatatt
     7261 tttcgctgcg gatgtacgcc tctttgcgct ccacaccgta gtaccctgcc tgattcacca
     7321 caatttgctg cacggtttca aacgggttga agttaaattc ctgcggcacc agtcccaact
     7381 gacgtttagc gttcacgaca tctttctcga gatcgtaacc aaatacgctg acccgcccgg
     7441 aggttttatt taccagagag ctgataatac cgatagtggt cgatttcccg gccccgttcg
     7501 gcccgagaag cgcataaaaa tcacccgctt cgacctgcaa atctattcca cgaagcgcct
     7561 gaacgccgcc tggataggtt tttttaagct gttgaagttc cagtgcaatg gtcataaatt
     7621 tttacttacc ttacgttctt acactttatc tgtggtttaa atcgtcccgg agttgcccta
     7681 tattagccaa acgtaattat ttggttacag gtcgttaacc tccatgaaag acatagatac
     7741 actcatcagc aacaatgcac tatggtcaaa aatgctggtg gaagaggatc ccgggttttt
     7801 tgagaaactg gcacaagcgc aaaaaccgcg ctttctatgg attggatgtt ccgacagtcg
     7861 cgttcctgca gaacgtttaa ccggtcttga gccgggcgaa ctctttgttc accgtaatgt
     7921 tgctaacctg gtcattcaca ccgacctgaa ctgcctttcc gtggttcagt atgcagtgga
     7981 tgtactcgaa gttgaacaca ttattatctg tggccactac ggttgcggcg gcgtacaagc
     8041 cgccgttgaa aacccggaac tggggcttat caacaactgg ctgctgcata tccgcgatat
     8101 ctggttcaaa catagctcat tgctcggcga aatgccgcaa gagcgccgtc tggatacctt
     8161 gtgtgaactg aacgtcatgg aacaggttta taacctaggc cactccacca ttatgcaatc
     8221 agcgtggaaa cgcgggcaga aagttaccat tcacggctgg gcctacggca ttcacgacgg
     8281 cttgctgcgt gatctggatg tcacagcgac caaccgcgaa acccttgagc aacgttaccg
     8341 tcacgggatt tccaacctca agctgaaaca cgccaaccac aaataaaaat gccatgccgg
     8401 atgcaacaca tccggcaact tcacacttac tcgtcccgca gaatcacttt gccgatatac
     8461 ggcagatgac ggtaacgctg tgcgtaatca atgccgtaac ccaccacaaa ctcatccggg
     8521 atcgagaaac cgataaattc taccgggacg ttcacttcac gacgggacgg tttatccagc
     8581 agcgtacaaa tcgccagcga cttcggttcg cgcaggctta agatctcacg cactttcgac
     8641 agtgtattcc ccgagtcgat gatatcttca acaatcagca cgtccttgcc acggatatct
     8701 tcatccagat ctttgaggat tttcacatca cgggtggtgg acatgccgct accgtagctg
     8761 gaggcggtca taaagtcgac ttcatgagat acctgaactt cacggcacag gtccgccata
     8821 aacataaatg agccacgcag cagacccacc agcaccatat cgctgccgct gtctttgtaa
     8881 cgctcagtaa tctgacgacc cagttcggcg atacgcgctt taatctccgc ttcggggatc
     8941 attacttcta cagtatgttt catatctcta accatatgat ttaaaaacaa atcacttcgc
     9001 acgggcaggt tttggcaccg caacaaagcg caagacacgc agtataccag taaaactatt
     9061 cattcgcgga atctgttaat aaagctcgaa ttgtgatgac ggtcacacat gttaaaacct
     9121 ataattaatt gctactaaaa tattaatgaa ttgaaatggt gtctctttat ggcaattaac
     9181 aatacaggct cgcgacgatt actcgtcacg ctaacagccc tttttgcagc gctttgcggg
     9241 ctgtatctac tcattggcgg aggctggctg gtcgcgattg gcggctcctg gtactaccct
     9301 atcgctggcc ttgtgatgct cggcgtcgcc tggatgctgt ggtgcagtaa acgcgccgcg
     9361 ctttggctat acgcagccct gctgctcggc accatgattt ggggcgtctg ggaagttggt
     9421 ttcgacttct gggcgctgac tccgcgcagc gatattctgg tcttcttcgg catctggctg
     9481 atcctgccgt ttgtctggcg tcgcctggtc attcctgcca gcggcgcagt tgccgcactg
     9541 gtggtggcac tgctgattag cggtagtatc ctgacctggg ccggatttaa cgatccgcag
     9601 gaaatcaacg gcaccttaag cgccgatgcc acacctgcgg aagctatctc acccgttgcc
     9661 gatcaggact ggcctgccta tggtcgcaat caggaaggtc aacgcttttc acctctgaaa
     9721 caaattaacg ccgataacgt ccataatctg aaagaagcct gggtgttccg tactggcgat
     9781 gtgaagcagc cgaacgatcc gggcgaaatc accaatgaag tgacgccgat taaagtgggc
     9841 gacacccttt acctgtgtac cgctcaccag cgcctgtttg cgctcgatgc cgccagcggc
     9901 aaagagaaat ggcattacga tcctgagctg aaaactaacg agtctttcca gcacgtaacc
     9961 tgccgtggcg tctcttatca tgaagccaaa gcagaaacgg cttcgccgga agtgatggcg
    10021 gattgcccgc gtcgtatcat tcttccggtc aatgatggtc gactgattgc gattaacgct
    10081 gaaaacggca aactgtgcga aaccttcgcc aataaaggcg tgctcaatct gcaaagcaat
    10141 atgccagaca ccaaaccggg tctgtatgaa ccgacttcgc caccgattat caccgataaa
    10201 accatcgtga tggccggttc agttaccgat aacttctcaa cccgcgaaac gtctggcgtg
    10261 atccgtggtt ttgatgtcaa caccggggag ctgctgtggg cttttgatcc cggcgcgaaa
    10321 gatccgaacg caatcccgtc tgacgaacac acctttacct ttaactcgcc aaactcctgg
    10381 gcaccagcgg cctatgacgc gaagctggat ctggtctatc tgccgatggg cgtgaccacg
    10441 ccggatatct ggggcggtaa ccgcacaccg gaacaggaac gttatgccag ctcgattctg
    10501 gcgctgaatg ccactaccgg gaaactggcg tggagctacc agaccgttca ccacgacctg
    10561 tgggacatgg atcttccggc acagccgacg ctggcggaca tcaccgttaa tggtcagaaa
    10621 gtgccagtta tttacgctcc ggcgaaaacc ggcaacattt ttgtgctcga tcgtcgcaat
    10681 ggcgaactgg tggttccggc cccggaaaaa ccggtgccgc aaggtgctgc gaaaggcgat
    10741 tacgttactc cgacccagcc gttctctgaa ctgagcttcc gtccgaagaa agatttgagc
    10801 ggtgcggata tgtggggagc caccatgttt gaccaactgg tgtgccgcgt gatgttccac
    10861 cagatgcgct atgaaggcat tttcacgccg ccatctgaac agggcacgct ggtcttcccg
    10921 ggtaacctgg gaatgtttga atggggcggg atttccgttg atccaaatcg tgaagtggca
    10981 attgccaacc caatggcact gccgtttgtt tcgaaactga tcccacgcgg tccgggtaac
    11041 ccgatggagc agccgaaaga tgccaaaggc acgggtacgg aatccggcat tcagccacag
    11101 tacggtgtac cgtatggtgt cacgctcaac ccgttcctct caccgtttgg tctgccgtgt
    11161 aaacagccag catggggtta tatctcggcg ctggatctga aaatcaatga agtggtgtgg
    11221 aagaaacgta ttggtacgcc gcaagacagt atgccgttcc cgatgcctgt accggtgccg
    11281 ttcaatatgg gtatgccgat gctgggcggg ccaatctcca cggcgggtaa cgtgctgttt
    11341 atcgctgcta cggcagataa ctacctgcgc gcttacaaca tgagcaacgg tgaaaaactg
    11401 tggcagggcc gtttaccagc gggtggtcag gcgacgccaa tgacctatga agtgaatggc
    11461 aagcagtatg tggtgatctc cgcaggcggt cacggttcat ttggtacgaa gatgggcgac
    11521 tatattgtgg cttatgccct gccggatgat gtgaaataac cataatgccc ggagagatcc
    11581 gggcatattt ccgaatacgg tctttttata ccgtaaaccc taacatcatc cccgtatctt
    11641 catgctccag cagatggcag tgcgccatat aagcatgttc tttcggtgcg ttgtgattaa
    11701 acttcaccag cacttcactg acattgcctt ctaccttaac ggtatctttc cagcccgcgc
    11761 gatgagccgc tggcggtttg ccattttctg acaagatacg gaactgcatg ccgtgaatat
    11821 ggaacggatg cagcatcatg tcgcccacgc cagagataac ccaacgttcg tactgccctt
    11881 tcgccgccgc aaacatcggc ttattcatat caaacgcctg accgttgatt ttattggcat
    11941 ggtggaaatc gaacttcccg ccgtggttca tatgattcat attgccgtgc cccatatggc
    12001 ccatcatctg gctgtgatcc attccggcca tcgcctgatc gccatatttc tccattagca
    12061 tctgcatccc catcatatcg agcatcgggt ccatagaaag ttgcagcttg cgtaccgtca
    12121 gcccttccag cgaaggtaac gcaggcaggc tacttaatgt gtcaggcaaa gcaccggagg
    12181 cactaatagc aatcggctga atccgcatta ccggatgcgg cttgtcaaac ggcgcaatcg
    12241 ccatccccat ctggctgacc ggtagcgtca ccagatcaaa gggtttgttg tcgttaacct
    12301 ccaccagcac ttcaaaacgc tcgcccatca gcaccggcag ctcgctcacc ttcaccggtt
    12361 caggtagcag accaccgtcg ctggcaatca catacagcgg gcgattgtcg ctggtggcga
    12421 aattgagcga gcgggcgtta cagccattga gcaaacgcag gcgcagccag ccacgcgggg
    12481 cagcgtgttg tgggtagatt gcaccgttgg tcagcagcgt atcgccaaac cagcccacgg
    12541 cggcggtcat cacatccagt tgataatcaa tctgcccatc agcgttaaat tttttatcct
    12601 gaacaatcac cggaacatca tcgatacccc actgttttgg cagcattaat ttcaggatct
    12661 cgtcatcttc aattaccacc agcccagcca accccatcgc cacctgtcgc ccggtcttgc
    12721 catgttgatg tggatggaac cagcaggtag cggcaggttg atcaacgttc aacgtcaccg
    12781 tacgtttgcc gcctggcgga ataattccct gcgggccgcc gtcgacgtca cccggtactt
    12841 ccagcccgtg ccagtgcaac gtcgtctctt ccgccagttg gttatagata tcaaccgtta
    12901 ccgctttgcc gcgctgtaat ttcaccgccg gccccagcag attgccgtta tagccccagg
    12961 tagttgcagt tttctcgcca aaggtagact gacctgcgcc aatagttaac tgaatgcgat
    13021 tacgggcatc ggtcgtgagc aaatcaggga tcggtaacgt tgggcgttcc gccgcaaaga
    13081 ctgcgcggct ccacagcggc aaggctgaag ccacacccag cgcgacggaa tattttaaga
    13141 aatcacgacg ttgcatagtt atttccttat tcttaagcag gcgaaacaaa atcaaacgtt
    13201 gagcatagtc cttcccctta cgggaaggtc aagccgcaga cacaatttaa tcgacaataa
    13261 tggtcgtcgc ctcgctcgca gtgtgctaac gtttatcttc tttaagcccc tggtagaagc
    13321 aatgaagacg tttttcagaa cagtgttatt cggcagcctg atggccgtct gcgcaaacag
    13381 ttacgcgctc agcgagtctg aagccgaaga tatggccgat ttaacggcag tttttgtctt
    13441 tctgaagaac gattgtggtt accagaactt acctaacggg caaattcgtc gcgcactggt
    13501 ctttttcgcc cagcaaaacc agtgggatct cagtaattac gacaccttcg acatgaaagc
    13561 cctcggtgaa gacagctacc gcgatctcag cggcattggc attcccgtcg ctaaaaagtg
    13621 caaagccctg gcccgcgatt ccttaagcct gcttgcctac gtcaaataat ccctgatacc
    13681 tttttgtaga aatattgacc gtgcatccgc ggtcaatgtt agctattatg ttgcgccctt
    13741 tttttacggg tgttaacaaa ggaggtatca acccatggcc gaaaaaaaac agtggcatga
    13801 aacgctacac gaccagtttg ggcagtactt tgcggtagat aacgttctgt atcatgaaaa
    13861 gaccgatcac caagatctga tcatttttga gaacgctgca tttggtcgcg taatggcgct
    13921 ggatggcgta gtacaaacca ccgagcgcga cgagtttatc tatcatgaga tgatgaccca
    13981 tgttccgcta ctggcccacg gtcacgcgaa acatgtgctg attatcggcg gcggcgacgg
    14041 tgccatgctg cgtgaagtaa cccgacataa aaacgttgag tcaatcacga tggtggaaat
    14101 cgatgcgggt atcgtgtcgt tctgccgtca gtatctgccc aaccataacg ccggtagcta
    14161 cgacgatccg cgctttaagc tggtgatcga cgatggcgtc aatttcgtta atcaaaccag
    14221 ccagaccttt gatgtcatta tctccgactg caccgatcct atcggtcccg gcgaaagtct
    14281 gttcacctca gcattttatg aaggctgcaa acgttgcctg aatcctggcg gtatcttcgt
    14341 cgcacaaaac ggcgtctgct ttttacagca ggaagaagcc atcgacagct atcgcaaact
    14401 cagccattac ttcagcgacg ttggctttta tcaggcggcg atcccgacct attacggcgg
    14461 tatcatgact tttgcatggg cgacagataa cgacgcctta cgccatctct caaccgaaat
    14521 tattcaggcg cgttttctcg cttctggcct gaaatgccgt tattacaatc cggcaatcca
    14581 tacggcagct tttgccttac ctcagtatct gcaagacgca ctggcttcac agccgtccta
    14641 aggagaagat aagaaattga aaaaactgaa actgcatggc tttaataatc tgaccaaaag
    14701 tctgagtttt tgtatttacg atatctgcta cgccaaaact gccgaagagc gcgacggtta
    14761 tattgcttat atcgatgaac tctataatgc caaccgtctg accgaaatcc tgtcagaaac
    14821 ctgttccatt atcggggcca atattcttaa catcgcccgc caggattacg aaccacaggg
    14881 tgccagcgtc actattctgg tgagtgaaga accggttgac ccgaaactca tcgacaaaac
    14941 agaacacccc ggcccactgc cagaaacggt cgttgcccat ctcgataaaa gtcatatttg
    15001 cgtacatacc tacccggaaa gtcatcctga aggcggttta tgtaccttcc gcgccgatat
    15061 tgaagtctct acctgcggcg tgatttctcc gctgaaggcg ctgaattacc tgatccacca
    15121 gcttgagtcc gatatcgtaa ccattgatta tcgcgtgcgc ggttttaccc gcgacattaa
    15181 cggtatgaag cactttatcg accatgagat taattcgatt cagaacttta tgtctgacga
    15241 tatgaaggcg ctgtatgaca tggtggatgt gaacgtctat caggaaaata tcttccatac
    15301 caagatgttg cttaaagagt tcgaccttaa gcactacatg ttccacacca aaccggaaga
    15361 tttaaccgac agcgagcgcc aggaaattac cgctgcgctg tggaaagaaa tgcgcgagat
    15421 ttattacggg cgcaatatgc cagctgttta acggctctgg cggagctccc aggctccgcc
    15481 agatttattt acttctgctg cacgaaattg cggtaagccg ccacgacctg cagaaaatcc
    15541 tcaacgccgc atagcgacag gctttcttcg tcgtagtagt tcatcccttc ttccatttca
    15601 tcgccagcga attccagttg attggcgcga accatcacct cttcaccgtc catccacagg
    15661 gtgtattcat gccctgcccg ttgccaggac cgttcgctac ctttcagtgc gtgcgctgct
    15721 tgttccactt catcaagcaa agccaggttt tctttcacct cttcattaaa ccagtgcccg
    15781 accacttcat gccccatgga catacgcacc tttaccactc cggtaatatc gcgcagaaat
    15841 tcgtaatcca tagtgtgttc ctctgctccc ggcaatgcgc cgaaatcgca ttgcgctatt
    15901 gatgtaatta ttgcagcctt accggagaaa aacagcggaa cggcgatggt ttagaaataa
    15961 aaaatgcccg ggaatctcat tcaccgggca ttgtgtcgtt tatgcgcagc gcgtgcgctt
    16021 acttattaca ccgcagtctg gaaaatcacc ccatcggctt tttcggtgta ctgagaaagc
    16081 tggttgaagt tcagataacg ataagtatca acggcagttt tatctacttg cgcaacgtag
    16141 gtctggtact cttccggcgt cggcagtttg ccaatcagcg ccgcaacagc cgccagttct
    16201 gcagaagcca ggaagacatt cgcgccagtc cccagacggt tcgggaagtt acgggtagag
    16261 gtggaaacca ccgtcgcacc gtctgctaca cgcgcctggt tacccataca cagagaacag
    16321 cccgggatct cgatacgagc cccgctctta ccgaagacgc tgtagtagcc ttcttcagtc
    16381 aactgtgcgg cgtccatgcg ggttggcggt gccacccaca ggcgggtcgg caactggcct
    16441 ttgtgcgcat ccagcagttt acccgcagca cggaagtgac cgatgttagt catgcaagaa
    16501 ccgataaaca cttcgtcgat cttctcgccc tgtaccgcag acagcggacg tgcgtcgtcc
    16561 gggtcgttcg gtgcacagag gattggctct ttaatatccg ccagatcgat gtcgatcact
    16621 gccgcgtatt cagcatctgc atcggcttcc agcagctcag gattcgccag ccatttttcc
    16681 atgccctgaa tacgacgttc gagggtacga cgatcgccat aaccttccgc gatcatccac
    16741 ttcagcagga cgatgttaga gttcaggtat tcgatgatcg gttctttgtt cagcttgata
    16801 gtacaaccag cggcagaacg ctcagcggac gcatcggtca gttcaaatgc ctgctcaact
    16861 ttcagatccg gcagaccttc aatttccaga atgcgaccag agaagatgtt tttcttgcct
    16921 ttcttctcaa cggtcagcag accttgtttg atcgcataca gcgggatcgc gtgtaccaga
    16981 tcgcgcaggg tgatgcccgg ctgcattttg cctttgaagc gcaccagaac ggattccggc
    17041 atatccagcg gcattacgcc tgttgcggct gcaaatgcca ccagaccaga acccgccggg
    17101 aaagagatac cgatcgggaa acgggtatgg gagtcaccac cggtaccgac ggtatccggc
    17161 agcagcatac ggttcagcca ggagtggatt acgccgtcac ccggacgcag cgacacacca
    17221 ccacggttca taatgaagtc cggcagcgtg tggtgcgtgt tcacgtcaac tggcttcgga
    17281 tacgccgcgg tatggcagaa agactgcatc accaggtcag ccgagaagcc caggcacgcc
    17341 aggtctttca gttcatcacg ggtcatcggg ccggtggtgt cctgtgaacc tacagaagtc
    17401 attttcggtt cgcagtacgc gcccggacga atacctttca caccgcaggc acgacctacc
    17461 attttctgag ccagcgagaa gccgcgatcg ctctcagcga catctttcgc ctgacggaac
    17521 acatcactgt gcggcagacc aagtgcttca cgcgctttgg tggtcaggcc acgcccgata
    17581 atcagcggaa tacggccacc agcacgcact tcatcaatca gcacgtcggt tttcagttcg
    17641 aaggtcgcca gcagttcgcc ggtttcgtgg ttacgcactt cacctttgta cgggtaaacg
    17701 tcaatcacgt cgcccatgtt caggttagag acgtcgactt cgattggcag tgcacccgcg
    17761 tcttccatcg tgttaaagaa gatgggtgca attttaccgc cgaggcacaa accaccgccg
    17821 cgtttgttcg gcacatgtgg aatatcatcg cccataaacc acagaacgga gttcgtggcg
    17881 gatttacgcg atgaacccgt acccacaacg tcaccgacgt acgccagcgg gaaacctttc
    17941 tgttgcagag cttcgatttg cttgatcggg ccaacaacac ctggctggtc tggctcaata
    18001 ccttcacggg cgtttttcag catcgccagc gcgtgcagtg ggatatccgg gcgcgaccac
    18061 gcatccggtg ccggagaaag gtcatcagtg ttggtttcgc cagtgacttt gaagacggta
    18121 acggtcagtt tttcagccag cgccgggcga ttcaggaacc attcggcatc cgcccaggac
    18181 tgcattacct gcttcgcata ttcgttgcct gctttggctt tctcttctac gtcatagaag
    18241 ttatcgaaca tcagcagtgt gtgagaaagt gctttggcag cgatcggtgc cagtttggca
    18301 tcatccagcg cgtcgatcag cggatgaatg ttgtaaccac cctgcatggt acccagcagt
    18361 tcgatggctt tttccggagt cagcagaggg gatttggctt cgcctttagc gatagcagcc
    18421 aggaagcctg ctttgacata ggcggcttca tcgacgccag ggggaacacg gttggttaat
    18481 agatctaaca ggaattcttc ttcgcccgcg ggcgggtttt tcagcagctc tacaagtgcg
    18541 gccatttggt ttgcatccag gggtttgggc gcaatcccct cagcggcacg ctcagctacg
    18601 tgcttacggt attcttctag cacgacggtt ctcctcgctc tcattgtcat agtgcggcag
    18661 gcgattctct tcacgctcct gtgagacagc agtttgtagg gtaaatgccc agtaccgcaa
    18721 cgggcagaat agcaggattt tgacgacgtg ttaatctgtt tacaaaaaag caacataaaa
    18781 aagtggctga atcgttaagg atggtctaga gattgtttcc ctccgtaaaa acttcgttaa
    18841 ttcgcatggt gataatcacc tttctcaacg aatcccaaga ttagtcaaaa tttaaactac
    18901 cgcctcttta tactcggatt aacagcacct gcgggtggca gttcgccgac cattgcgatt
    18961 tccttgagat ccgaattatg aaaatgactt tgccgtttaa accccatgtg ctggcactaa
    19021 tttgcagtgc cgggctttgt gccgcctctg ccgggctata tataaaaagc cgcacagtgg
    19081 aagcgcctgt ggaaacgcaa tcgacacaac agactgcgcc tgacatcacc gcagttacgc
    19141 ttcctgcaac ggtttccgca ccgcccgtaa cgcccgccgt cgtcaaatcc gcattcagca
    19201 ctgcacaaat cgatcaatgg gttgcgcctg tcgcgctgta ccccgattct ctgctttcac
    19261 aagtgttaat ggcatcaacc tatccggcaa acgttgctca agcagtgcaa tggtcgcacg
    19321 ataatccact taaacaaggc gatgctgcta ttcaggcggt atctgaccag ccgtgggacg
    19381 ccagcgttaa atcactggtg gcctttccac aattgatggc attgatgggc gaaaacccgc
    19441 aatgggtgca aaacctgggc gatgcttttc tggcgcagcc gcaggacgtg atggactcgg
    19501 tacagcgatt gcggcaactg gcgcaacaaa ccggctcgct gaagtcatca accgaacaga
    19561 aagttattac cacaacgaag aaagctgtac cggtaaaaca gacagtcacg gcacccgtca
    19621 taccatccaa taccgtttta actgccaacc ccgtcattac agagccggca acaaccgtca
    19681 tttccattga gcccgccaat cctgatgtgg tctatattcc caactacaac ccaaccgtgg
    19741 tttacgggaa ctgggccaat actgcgtatc cgccggttta tctgccacca ccagccggag
    19801 aaccgtttgt tgacagcttt gtgcgcggat tcggctatag catgggcgtt gctaccacgt
    19861 acgcactatt tagcagcatc gactgggatg acgacgatca tgaccatcat catcatgacg
    19921 atgatgatta tcatcaccac gatggcggtc atcgtgacgg taatggctgg caacacaacg
    19981 gcgacaacat caatatcgac gtcaacaatt tcaaccgtat caccggtgag catcttactg
    20041 ataagaatat ggcatggcgg cacaatccaa actaccgtaa tggtgtgccc tatcatgatc
    20101 aggatatggc aaagcggttt catcaaaccg atgtcaacgg cggaatgagt gccacgcagc
    20161 tacctgctcc aacacgcgac agccagcgtc aggcggcagc aagtcagttt cagcaacgaa
    20221 cacacgccgc ccccgtcatt acacgagata cccaacgtca ggcagcggca cagcggttta
    20281 atgaagctga acactatggg agctatgacg acttccgcga cttcagccgt cgccaaccac
    20341 tgacccagca acaaaaggac gccgctcgtc agcgttatca atcggcctct cctgagcagc
    20401 gccaggcggt tcgcgagaaa atgcagacta acccacagaa ccagcagcga agagaggcag
    20461 cgcgtgagcg tattcagtct gcaactcccg aacagcgcca ggtgtttaag gaaaaagtac
    20521 agcagcgccc actgaaccaa cagcaacgtg ataacgcccg ccagcgtata caatcggcat
    20581 cacctgaaca acgtcaggtt tttcgggaaa aagttcagga gagccgccca caacgtctaa
    20641 acgacagtaa ccatactgtc aggctgaata acgagcaacg gtcagcagta cgcgaacgtc
    20701 tctctgagcg cggagcaagg cgacaggaaa ggtaaattgc agacgtaaaa aaagcggcgt
    20761 ggttagccgc ttttttaatt gccggatgtt ccggcaaacg aaaaattact tcttcttcgc
    20821 tttcgggttc ggcaggtcgg taatgctacc ttcgaacact tctgccgcca ggcccacaga
    20881 ctcgtgcaga gtcgggtgcg cgtggatggt cagtgcgatg tcttcagcat cacaacccat
    20941 ttcgattgcc agaccgattt cacccagcag ctcaccgccg ttagtaccga caatcgcacc
    21001 accgatcaca cggtgagatt ctttgtcgaa aatcagcttg gtcataccgt ctgcgcagtc
    21061 ggaagcgata gcacgaccag aagcagccca cgggaaggtg gcggtttcat agctgatgcc
    21121 tttctctttc gcttctttct cagtcagacc tacccatgca acttctggtt cggtataggc
    21181 gatagacggg ataactttcg gatcgaagta gtgtttttta ccggcgataa cttcagcggc
    21241 aacgtgacct tcgtgaacac ctttgtgtgc cagcatcggc tgaccaacga tatcgccgat
    21301 agcaaagatg tgcggtacgt tggtacgcag ctgtttgtca acgcggatga aaccacggtc
    21361 gtccacttcc acgccagctt tgcctgcgtc gaggttttta ccgttcggca cacgaccaat
    21421 cgctaccagc acggcgtcgt aacgctgcgg ttcagcgggt gcttttttgc cttccatcgt
    21481 cacataaata ccgtcttctt tcgcttcaac ggcggtaact ttggtttcca gcatcaggtt
    21541 gaatttcttg ctgatacgct tggtgaagac tttaacgatg tctttgtcag ctgccgggat
    21601 aacctggtcg aacatttcaa ccacgtcaat ctgtgaaccc agcgcgtggt atacggtgcc
    21661 catttccaga ccgatgatac cgccacccat taccagcagg cgttctggta cttctttcag
    21721 ttccagcgcg tcagtggagt cccagatacg cggatcttca tgcggaataa acggcagttg
    21781 gatcgggcga gaacccgctg caatgatcgc attgtcgaag ttgatcacgg ttttgccgtt
    21841 ctcaccttca acttccaggg tgttagcccc ggtgaattta cccagaccgt tgaccacttt
    21901 gactttgcgg cctttcgcca taccagccag accaccggtc agctgattga tcactttctc
    21961 tttccaagta cgaatcttgt caatatcggt tttcggttcg ccgaagacga taccgtgttc
    22021 agccagcgct ttggcttctt cgataacttt tgctacgtgc agcagtgctt tagaagggat
    22081 acagccgacg ttcaggcaaa caccgccaag ggtgttgtaa cgttctacga ttacggtttc
    22141 cagacctaaa tcagcgcaac ggaaggcagc ggagtaacct gcggggcctg ccccaagtac
    22201 cacgacctga gttttgattt cagtactcat catgacctct atatatttat ctccggcggt
    22261 catacccgtc gtctttcagg ccgcagcggc gttagcttca cgccctcacc ccagtcactt
    22321 acttatgtaa gctcctgggg attctggcgc ttgctacctt gctgcaccca gaaatccata
    22381 gggtatgtga agtaataccc taaccaccac cgggtcgttc tatccgtcgg tattttacaa
    22441 aattgttaac aatttttaaa caacaaacgg caaccgattt gtctattcgc taataacctc
    22501 aatacattca tgagattacc agaaaaaagc cggccgttgg gccggctctt ttacttacat
    22561 caccagacgg cgaatgtcag acagcgtgtt gttaatgatg gtaatgaaac gggcaccatc
    22621 agcaccgtcg atcacgcggt ggtcgaagga gagagaaatc ggcagcatca gacgcggcac
    22681 gaactcttta ccattccaca ccggctccat cgcggacttg gaaacgccga ggatagccac
    22741 ttccggcgcg ttcacaatcg gcgcgaagtg ggtagtaccc aggccgccga tgctggagat
    22801 ggtgaagcaa ccgccctgca tttcgcccgc agtcagctta ccgtcacgcg ctttcttaga
    22861 aatagtcatc agctcgcgag acagctcgat gatgcctttc ttgttgacgt ctttgaatac
    22921 cggaacaacc agaccgttcg gggtatccac cgccacaccg atgttgatgt atttcttcag
    22981 ggtcagacgc tgaccgtctt ccgacagcga actattgaag cgaggcatct gctcaagagc
    23041 tgcagcaacg gctttcatga tgaagacaac cggggtgagc ttcacatcca gcttacgttt
    23101 cgccgcttct tcgttctgct gtttacggaa cgcttccagc tcggtgatat cggttttgtc
    23161 gaagtgggta acatgcggga tcatcaccca gttacggctc aggttagcac cagaaatttt
    23221 ctggatacgg cccagttcca cttcttcgat ttcgccaaac ttgctgaagt ccaccttcgg
    23281 ccacggcagc atgccaggga taccaccgcc agtcgccgcc ggagctgctt ctgcacgttt
    23341 gatagcttct ttcacgtaag cctgaacgtc ttcgcgcagg atacgacctt tacgaccagt
    23401 gcccttcact ttcgccaggt taacgccaaa ctcgcgtgcc agacggcgga tcagcggagt
    23461 cgcgtgaacg taagcgtcgt tttcagcaaa ttcagatttg ccttcggctt tcgcagccgg
    23521 agctgctgcc ggggcttcag ctttcgctgc cggagccggc gctgccgctt cctgtttcgc
    23581 aggagctgcc gcaggcgctg cgccttcaac ttcgaagatc ataatcagcg agccagtttt
    23641 cactttatcg ccaacgttga ctttcagttc cttcacgacg cctgcaaacg gagccgggac
    23701 ctccatagaa gctttatcgc cttctacggt gataagcgat tgttcagcgg caactttgtc
    23761 gcccactttc accatcactt cggtcacttc aacttcgtca ccgccgatat ccggaacgtt
    23821 aacttctttc acgccagccg ctgatgcagg ggccgctgcc ggagctgctt cctgtttagc
    23881 cgccggagct gccgcgcctg cttcacccgc gacttcgaag accataatca gcgagccggt
    23941 agacactttg tcacccacgt tcactttgat ctctttcacg gtgccagcaa acggagccgg
    24001 aacttccata gaagctttat cgccttctac ggtgatcagc gactgttcag cttcaacttt
    24061 atcgccaact ttcaccagga tttcggtcac ttcaacttcg tcgctgccga tatccggaac
    24121 gttaacgtct tttgccgccg cagccgcggg tgctgctgcc ggagctgctt ctttcttctc
    24181 ttctgcctga gcaggtgcag cgtctgctgc accgtcggcg gaatcgaaaa tcataatcag
    24241 tgcgccggtc tgggttttat cgccaacaga gactttgatc tctttaacga tacccgcctg
    24301 cggagacgga acttccatag aggctttgtc gccttctacg gtgatcagcg actgttcggc
    24361 ttcaactttg tcgcccactt tgaccaggat ctcggtgatt tcaacttcat cagccccgat
    24421 gtccggtact ttgatttcga tagccattat tcttttacct cttacgccag acgcgggtta
    24481 actttatctg catcgatgtt gaatttggcg attgcgtcag caaccacttt cttatcgatt
    24541 tcgccacgtt tagccagttc gcccagcgcc gcaaccacga cataagaagc atcaacttcg
    24601 aagtggtgac gcaggttctc acggctgtcg gaacgaccga agccatcagt acccagtacg
    24661 cggtagtcgt cagccggtac gtaagtacgg acctgctcag cgaacagttt catatagtcg
    24721 gtagatgcca ctgccggagc gtcgttcatc acctgagcga tatacggaac gcgcggagtt
    24781 tccagcggat gaagcatgtt ccagcgttca caatcctgac catcacgcgc cagctcggtg
    24841 aaggaggtca cgctataaac gtcagaacct acgccgtaat ctttcgccag gatctctgcc
    24901 gcttcacgga cgtgacgcag gatagaaccg gagcccagca gctgaacttt acctttgcta
    24961 ccttcaatgg tttcgagttt gtagatacct ttacggatac cttcctcagc accttccggc
    25021 attgccggca tgtggtagtt ttcgttcagc gtagtgatgt agtagtaaac gttctcttgt
    25081 ttttcaccgt acatacgctc cagaccgtca tgcatgatga cagcaacttc gtaagcgtaa
    25141 gccgggtcgt aagagataca gttcgggata gtcagcgact gaatgtggct gtgaccatct
    25201 tcgtgctgca gaccttcacc gttcagggtg gtacgaccgg aagtaccgcc gatcaggaag
    25261 ccacgcgctt gctggtcgcc agccgcccag cacagatcgc cgatacgctg gaagccgaac
    25321 atcgagtaat agatgtagaa cggaatcatc ggcagattgt tggtgctgta agaggtcgcc
    25381 gctgccagcc aggaacaccc tgcgcccagc tcgttgatcc cttcctgcag aatctgacct
    25441 ttctcgtctt ctttatagta agcaacctgc tcgcggtcct gcgggatgta ctgctgaccg
    25501 ttcgggctgt aaataccaat ctgacggaac agaccttcca taccgaaagt acgcgcttcg
    25561 tcggcgatga tcggtaccag acgatctttg atcgacttgt tcttcagcat tacgttcagg
    25621 gcacgaacga aagcgatagt ggtagagatc tctttgctct gctcttccag cagcgcgccg
    25681 aagtcttgca ggctcggcag ctcaagcttc tcggtgaagt tcggctgacg gcttggcaga
    25741 taaccgtgca gtttctgacg ctgtgcgtgc agataggtat gctcttcaga accttccggg
    25801 aaggtgatgt acggcagttt ttcgatatct gcatcagaca ccggcacatt gaaacggtcg
    25861 cggatgtgac gcacgccgtc catgttcatt ttcttaacct ggtgcgcgat gtttttacct
    25921 tcagccgcgt cgcccatgcc gtaaccttta atggtatgag caaggattac tgtcgctttg
    25981 cctttggttt cctgcgcttt cttgaatgca gcgtagattt tcttcggatc gtgaccacca
    26041 cggttcagtg cccagatctg ctcgtcagtc cagtctgcaa ccagtgctgc ggtttcagga
    26101 tatttaccga agaagtgttc acgaacgtac gcaccatctt tcgatttgaa ggtctggtag
    26161 tcgccgtcaa cggtttcgtt catcagctgg atcagtttac cgctggtatc tttacgcagc
    26221 agttcatccc aacggctacc ccacatcact ttgataacgt tccagccagc accttcgaag
    26281 atgccttcca gttcgttgat gatcttgccg ttaccggtga ccgggccgtc aagacgctgc
    26341 aggttacagt tgataacgaa gaccaggtta tccagttttt cacgggtagc gatggtgatc
    26401 gcacctttgg attccggttc gtccatttca ccgtcgccga ggaacgcgta aacggtctgt
    26461 ttagaggtat ctttcaggcc acggtgttcc agatatttca ggaatttagc ctggtaaata
    26521 gcaccaatcg gccccagacc catagatacg gtcgggaact gccagaattc cggcatcagt
    26581 ttcgggtgcg gataggaaga gaggccattg ccgtgaactt cctgacggaa gttatccagc
    26641 tgctcttgag tcagacgacc ttccaggaaa gcacgagcgt acacgcccgg ggagatgtgg
    26701 ccctggaagt aaaccaggtc gccgccatcc tgctcgttgc gtgcacggaa gaagtggtta
    26761 aagcacacat cataaatggt tgcggaagac tggaaggacg ccatatggcc gcccagttcg
    26821 aggtcttttt tcgacgcacg cagcaccgtc atgatggcgt tccagcggat agctgaacga
    26881 atacggcgtt ccagttccag attacccgga tactccggtt gttcttcaac ggggatggtg
    26941 ttgatgtagt tgctgatacc tgtgcctgcg gctacgttta caccgccttt gcgggcttca
    27001 gcaagcagtt ggtcgatcag atactgagca cgctcaacac cttcttcacg gatgaccgat
    27061 tcgatcgcct ggagccagtc gcgagtttcg atcggatcca cgtcatttgg gaaacgttct
    27121 gacatgggtt attccttatc tatctaataa cgttgagttt tctggaacct gtcccattga
    27181 actctcgccg gaaagctcaa taagacaggt tctacgttta gttgccgcgc ttttatatac
    27241 gcttgattta caacatcttc tggataattt ttaccagaaa aatcactaat tctttcgttg
    27301 ctccagacga cgcagagaac gctcacggcg gctctcttca cgacttctgt cgagcaaaat
    27361 ttcttcgata aaggccagat ggcgatgcga tgcttcgcgc gcttcttccg gcttaccggc
    27421 cataatcgct tcaaatatgc gggtgcggtg actactcacc agcggcagca tctcgcgacg
    27481 cgaatagagc aattcgaagt tctggcggac attctgggcc aacatcggct ccatacacct
    27541 tagcagatga agcagaacca cattgtgggc cgcttcggtg acggcaatct gatactggag
    27601 tacggcgttt gattccgcgt ccagatcgcc agactgctgc gccagctcta tggcgtggtg
    27661 gagttcacgg atgcgttcct tgtcttcatc ggtactacgc agcgcggcgt aataagcggc
    27721 gataccttcc agggcgtgtc gtgtttcgag caagtcatac tgtgattcag gatggtcgga
    27781 gagcagctcc accagcggat cgctgaagct ttgccatagg ctgctctgga caaaagtgcc
    27841 gccaccctgg cgacgaagca acaagccctt cgcttcgaga cgttgaatcg cctcacgcaa
    27901 ggagggacgg gagacgtcga actgttttgc cagttcgcgt tccggtggga gtttttcgcc
    27961 cgggcggaga gtgccttcga gaatcaaaaa ctccagttgc tgctcaatca catcggagag
    28021 ttttggttgg cggattttgc tgtaggccat gagttcctgt cttaagccac ttgccgaagt
    28081 caattggtct taccaatttc atgtctgtga cgctaaagta acaaagtatt caccttatgt
    28141 ccatacaggt tttgattgaa atcatgaaac tgtgcacatt ttaacaactt gacatatata
    28201 acgtttcaaa attgtaacta tgcacaaatg tagactttac gtaggaaagg gattttttaa
    28261 cgattattaa actataaaaa tccgaattga accgattcac ttaccaattt tttgtttttt
    28321 aagtcaataa aaacgaattt aaattcattc tgcatattga gaggggttga ggctgagctt
    28381 tacaaacggt ttctttttaa gcaactcatc ttcaaccatg cataaagcgg gtgcattcgc
    28441 tgccgcatac cattattctt gatctgacgg aagtcttttt gtaacaattc aaacttcttt
    28501 gatgtaaaaa aattaataca acaaacggaa ttgcaaactt acacacgcat cactgcgtag
    28561 atcaaaaaaa caatcaccgc acgaggtttc atgatggaag gtcaacagca cggcgagcag
    28621 ctaaagcgcg gccttaaaaa ccgccatatt cagcttatcg cgctgggtgg cgcgataggg
    28681 acagggctat tcctgggtag cgcctccgta atacagtccg cagggccagg gattatcctg
    28741 ggttacgcca ttgctggttt tatcgccttt ctgatcatgc gtcagctggg tgaaatggtg
    28801 gtcgaagaac ctgtcgcagg ctcctttagc cactttgctt ataaatactg gggcagtttt
    28861 gccggtttcg cctctggctg gaactactgg gtactgtacg ttttagttgc catggcagag
    28921 ctgactgccg tgggtaaata cattcagttc tggtatccgg aaatcccaac ctgggtttct
    28981 gccgccgtat tctttgtggt gattaacgcc atcaacctga ccaacgttaa agtgtttggc
    29041 gagatggagt tctggtttgc cattatcaaa gttattgcga tagtagcgat gatcatcttc
    29101 ggcggctggc tgctgttcag tggcaacggc ggcccgcagg cgagcgttag caacctgtgg
    29161 gatcaaggtg gtttcctgcc gcacggcttc accggactgg tgatgatgat ggcgattatc
    29221 atgttctcgt tcggtggtct ggaactggtg gggatcaccg cagcagaagc tgataacccg
    29281 gagcaaagta tcccgaaagc gactaaccag gttatctacc gcatcctgat tttctatatt
    29341 ggttcgttag ccgttctgct ctcactgatg ccgtggaccc gcgttaccgc cgataccagt
    29401 ccgtttgtgc tgatcttcca cgagttaggc gatacctttg tggcgaatgc gctgaacatc
    29461 gtggtactga ctgcggcgct ctccgtgtac aacagctgcg tatattgcaa cagccgtatg
    29521 ctgtttggtc tggcacaaca gggtaatgcg ccaaaagcgc tggcgtctgt cgataaacgc
    29581 ggcgtaccgg ttaacaccat tctggtgtct gcgctggtta cagcattgtg cgtattgatt
    29641 aactatcttg ctccggaatc cgcatttggc ctgttaatgg cactggtggt atccgcactg
    29701 gtgatcaact gggcgatgat cagtctggcg catatgaagt tccgtcgcgc caagcaggaa
    29761 caaggcgtgg taactcgctt ccctgctctg ctttatccgc tgggtaactg gatctgcctg
    29821 ctgtttatgg cggcggtact ggtgattatg ctgatgaccc caggaatggc gatttcggta
    29881 tacctgatcc cggtatggct gatcgtgtta ggtatcggct atctgtttaa agagaaaacc
    29941 gccaaagccg taaaagcgca ttaatctctc tacgccctca cccatgcagg gtgagggcaa
    30001 taatctttac accaacgctc cgtaaatcgt cagtagcgca atcaccacca cgaccacgaa
    30061 cgaggttttc ttcgccattg aaaccgctgc cttcggcgtc tccaccttat cgacatgcgg
    30121 ttcacgcgcc agagagaact gcgccagacg cgttaacacc tgatactgcg aagtatggaa
    30181 gtcacccagc gaagcaaacc aggccggtaa cgctttctcc ccatgaccga tcaaggcata
    30241 taccacaccc gcaagacgaa ccggcaccca atccagtaca tgaagcacgg catcaatgcc
    30301 ggactgtaaa cgatgatgcg gcgtctggta tcgcgccagc cagtattgcc atgcacgcaa
    30361 aaacgcatac cccatcagcg taacgggtcc ccaggttccc cccacaatca accagaacag
    30421 cggtgcaaga taaaaacgaa agttaatcca cagcaatgca ttttgcagct cacgcaaata
    30481 ctcacgttcg tcgcagcctg ccgggacgcc gtgaatcatg gtgagttcgc cagccatcgt
    30541 ggcacgggca tggctatcat tacgtgaagc agctgtcaga taagcatgat aatgaagacg
    30601 aactttacct gcgccaatac acagcaaacc aatcagcagc cacaccagca gcgtgggaac
    30661 gttgaacaat actccctgca atgcgcgtaa cagtaaaaaa gtcacgccca tcgcaataat
    30721 ggtcatgcct aacgtgcgcc cgagagaaaa atgtttcacc cgccgaaaga acgcttcaag
    30781 acgatgatca agctgccagt gctcgcccaa cttaaacagg cgctcgaaaa ttaacaccag
    30841 taaggttgta aatagcgtca tgttgtctcc ttgctgacca gcgcacgaaa ccgtgcccaa
    30901 tcaaatgcag gaccgggatc ggttttccga tccggcgcaa tatcacaatg gcccgtcatg
    30961 ttattagcga tatcgggata gcgatctatc agtgcccgcg taaccgccgc aagctgttga
    31021 tactgcgcat cggtatacgc cagagtatcg gtgccttcaa gctcaatccc aatagaaaaa
    31081 tcattgcagc gttcacgccc ctgatactga gagactcccg catgccaggc acgtttatcg
    31141 aaaggaacat actggactat ttcaccatca cggcgaatca aacagtgagc ggagacgcgc
    31201 aaatgggcga tctcagcaaa gaaaggatgt gcctgcggat caatagttcc agtgaataat
    31261 gcgtcgatcc acggaccgcc aaactcgcct ggcggcaggc taatattgtg caccaccagc
    31321 agggtgggtg tttcgtcatc cgggcggcaa tcgtaatgtg gtgagggaac gcggcgcgcg
    31381 ccaaccagcc acccctgttc taacaacatg caggatctcc ttatatgtgg tgctaatacc
    31441 cggttcagag tagcatgttt ctaccttatg attcgttagc tatctggagt tttaacatgc
    31501 cgcctcgccg ctataaccct gacacccgac gtgacgagct gctggaacgc attaatctcg
    31561 atatccccgg cgcggtggcc caggcgctgc gggaagattt aggcggaaca gtcgatgcca
    31621 acaatgacat tacggcaaaa cttttaccgg aaaactctcg ctctcatgcc acggtgagca
    31681 cccgcgagaa tggcgtattt tgcggcaaac gctgggttga agaggtgttt attcaactgg
    31741 caggcgacga tgtcaccata atctggcatg tggatgacgg cgatgtcatc aacgccaatc
    31801 aacccttgtt cgaacttgaa ggcccatccc gcgtgctgtt aacgggcgaa cgcactgcgc
    31861 ttaattttgt gcaaaccctt tcaggagttg ccagtaaggt acgccactat gtcgaattac
    31921 tggaaggcac caacacgcag ttgttggata cgcgcaaaac cttacctggc ctgcgttcag
    31981 ctctgaaata cgcggtactt tgcggcggcg gagcgaatca ccgtctgggg ctttctgatg
    32041 ccttcctgat caaagaaaat catattattg cctccggctc agtgcgccag gccgtcgaaa
    32101 aagcgtcctg gctgcacccg gatgcgccag tagaagtcga agtagagaat ctggaagaac
    32161 ttgatgaagc cctgaaagca ggagccgata tcatcatgct ggataacttc gaaacagaac
    32221 agatgcgcga agccgtcaaa cgcaccaacg gcaaggcgct actggaagtg tctggcaacg
    32281 tcactgacaa aacactgcgc gaatttgccg aaacgggcgt ggactttatc tccgtcggtg
    32341 cgctaactaa acacgtacaa gcactcgacc tttcaatgcg ttttcgctaa ttgctgacag
    32401 atttatccgc tccatcgcgg gcggataatc tccccttccc caactttctt cgtaacgcct
    32461 cgcaaatttc ttgtcttttg ctgcaacgaa ataaaaaacc tcggaagctg ctttcccgtt
    32521 tcgttttttg cccctcgctt gtgctgatgg cgtttgccaa agtagcgcca accaaatcaa
    32581 ggagcgaaac agatggacaa gcaacgcggt tttacactta tcgaactgat ggtggttatt
    32641 ggcatcattg ccattttaag cgccattggt attcccgctt atcaaaacta cctgcgcaaa
    32701 gctgcactca ccgacatgct acaaaccttt gtgccttacc gtacagccgt agagttgtgc
    32761 gcgctggaac atggtggatt agatacctgc gacggtggca gcaatggcat tccctcgcct
    32821 accaccaccc gctatgtttc agccatgagt gtggcaaaag gcgtggtgtc gctgaccggg
    32881 caagaaagtc tcaatggact aagcgtcgtc atgacaccgg gttgggataa cgcaaacggc
    32941 gtcaccggct gggcgcgcaa ctgcaatatt caaagtgaca gcgcattgca gcaagcctgc
    33001 gaagatgtct tccgctttga tgacgccaac taaggagcgg caatgaatat tccacagctc
    33061 accgccctgt gtctgcgtta tcagggagtc ttgctggatg ccagcgaaga ggtggttcat
    33121 gttgcggtag tcgatgcacc ttcgcatgag ttgctggacg cattgcattt cgctaccacc
    33181 aaacgtattg agatcacctg ctggacgcgc caacaaatgg aaggccacgc cagtcgcaca
    33241 caacagacat tgcccgtagc tgttcaggag aagcatcagc ccaaagcaga gttgctgact
    33301 cgaacgttac aatctgcgct ggaacaacgt gcgtctgata ttcatatcga accagcggac
    33361 aatgcctacc gcatccgctt gcgtatcgac ggcgtattgc atcctttacc ggatgtttca
    33421 ccggatgccg gagtcgcatt aaccgccaga ttaaaagtgc tgggaaacct ggatattgcg
    33481 gaacatcgcc tgccgcagga cgggcaattc actgtcgaac tggcaggaaa cgccgtctca
    33541 tttcgtattg cgaccttacc atgtcggggt ggtgaaaagg tggtattaag gttgttacag
    33601 caggtgaacc aggcactgga tgttaacacg ctgggaatgc agccgttaca actggcggac
    33661 tttgctcatg ccttgcaaca accacaggga ctggtgctgg taactggccc tacaggcagc
    33721 ggcaaaacgg tcacgcttta tagtgccctg caaaagctga ataccgctga cattaatatt
    33781 tgtagcgtcg aagatccggt tgagatcccc atagccggac taaaccagac gcaaatccat
    33841 ccgcgtgccg ggctcacctt tcagggcgtt ttgcgtgcgt tattgcgcca ggatcctgac
    33901 gtcatcatga tcggagagat ccgctatggc tttaacggca gaaattgcca ttaaagccgc
    33961 gcaaaccggt cacctggtgt tgtctaccct acacactaat tccacctgcg aaacgctggt
    34021 acgtttacag caaatgggag tcgcccgctg gatgctctca tcagcgctta cgctggtaat
    34081 agcccagcgt ctggtacgta aactttgccc acattgtcgc cagcagcaag gggagcccat
    34141 ccatattcca gtcaatgtat ggccgtcgcc gctgccccac tggcaggcac ccggttgtgt
    34201 acattgctac cacggttttt atggtcgtac ggccttattt gaagttctgc ccataacgcc
    34261 ggtcattcgt cagcttattt ccgctaatac cgacgttgaa tcgctggaaa cgcacgcacg
    34321 acaggcgggt atgcgtacgc tttttgaaaa cggctgcctg gccgtggagc aaggcttaac
    34381 cacctttgaa gagttaatcc gcgtactggg gatgccgcat ggcgagtaag caactctggc
    34441 gctggcatgg cataaccggc gacggcaatg cgcaagatgg gatgctatga gcagagagcc
    34501 gtgctttgct gctcatggca ctacagcaac agatggttac cccacttagc ctgaagcgaa
    34561 tcgccatcaa ttctgcgcag tggcgaggag ataaaagcgc ggaagtcatt catcaactgg
    34621 cgacgctact caaagccggg ttaacgcttt ctgaagggct ggcactgctg gcggaacagc
    34681 atcccagtaa gcaatggcaa gcgttgctgc aatcgctggc gcacgatctc gaacagggca
    34741 ttgctttttc caatgcctta ttaccctggt cagaggtatt tccgccactc tatcaggcga
    34801 tgatccgcac gggtgaactg accggtaagc tggatgaatg ctgctttgaa ctggcgcgtc
    34861 agcaaaaagc ccagcgtcag ttgaccgaca aagtgaaatc agcgttacgt tatcccatca
    34921 tcattttagc gatggcaatc atggtggttg tggcaatgct gcattttgtt ctgccggagt
    34981 ttgccgctat ctataagacc ttcaacaccc cactaccggc actaacgcag gggatcatga
    35041 cgctggcaga ctttagtggc gaatggagct ggctgctggt gttgttcggc tttctgctgg
    35101 cgatagccaa taagttgctg atgcgccgac cgacctggct tatagtgcgg cagaaattgc
    35161 tgttacgcat cccgattatg ggttcactga tgcggggaca aaaactcacg cagatcttta
    35221 cgattctggc gctgacacaa agtgcaggca ttactttttt gcagggcgta gagagcgtca
    35281 gagaaacaat gcgctgcccg tactgggtgc aacttctgac acaaatccag cacgatatca
    35341 gtaacggtca tcccatctgg ctggcgctaa aaaataccgg ggagtttagc ccgctctgtt
    35401 tgcaattagt gagaacagga gaggcatccg gctcgctgga cctcatgtta gacaacctcg
    35461 cccatcatca tcgggataac acaatggcgc tggcggataa cctcgcagcc ttactggaac
    35521 cgacgttgct gatcataacg ggaggaatta tcggtacgct ggtggtggcg atgtatctgc
    35581 caattttcca tttaggcgat gcgatgagtg ggatgggata acgctggcgt gttgctccac
    35641 accagcgttg gaagattaca ggttgttgaa gatgcggttt tcttgttcct gcacacggat
    35701 aaatgtggtg cgcttggtca gttctttcag gcgtgaagcc ccaacgtatg tacaagctga
    35761 acgcaggccg cccaaaatat cacgcgcggt attttcaacc gggcctcgca gcggcagctt
    35821 aacggtttta ccttctgctg cgcgatattc cgcaacgccg ccaacgtgac gtttcatcgc
    35881 agactcggag ctcatgccgt agaacagcat aaatttctcg ccgttctcct caacgatgcg
    35941 accgccgctc tcttcgtggc ccgccagcat gccgccaagc atgacgaaat cggcaccgcc
    36001 gccaaaggct tttgcgacat cgcccggcgt ggtgcagcca ccgtcgctga cgatcattcc
    36061 gcccagaccg tgcgccgcat cggcacattc gattaccgca gaaagctgcg gataaccgac
    36121 gcctgttttg acgcgagttg tacaaacaga acctgggcca atgccaactt taacgatatc
    36181 agcaccggag aggataagct cctcacacat ttcaccagtc actacgttgc cagcacaaat
    36241 ggttttggtc ggccacgctt cacgcgcttt cgcaacgaac tgcacgaagt gttcggaata
    36301 accattcgcc acatcaatac aaacgaagtt taatgccggg ttcaggtcga gaatctgttt
    36361 agttttttcg aaatccgcat cagacgtacc ggtagaaacc atcacatgtt tcagcacatc
    36421 agcggaagaa ttgttgataa acgcttgcca ctcttcgaca gaatagtgtt tatgcacagc
    36481 agtcaaaata tcaaaagaag ccagcgcaga ggccatagaa aatgtaccta cggtgtccat
    36541 atttgcggcg ataatcggca cgccggacca gctctgacct gaatgtttga aggtgaattg
    36601 acgttccagt tcaacatcgg aacggctttt aagagtggag cgtttagggc ggatgagaac
    36661 gtctttaaaa cctaacttca gatcttcttc aatacgcatg tgcggattcc tggggttaat
    36721 ggctataagg gtaagagcgc aactccagtg acgctatcat acgcactaat caatgcggcg
    36781 caagactgcg aaattgtctt tttttacgct acaatcccat aaatttacct ggtaaatagt
    36841 gagcgaacgt ttcatcaatt tcgctttcgt gcactcattt ttaacgtttt gataatcatg
    36901 atgaattccc gggaatataa attatgaggt atatagttgc cttaacggga ggcattggca
    36961 gtggcaagag taccgttgcc aatgcgtttg ctgatctcgg aattaacgtc attgatgccg
    37021 atattattgc gcgtcaggtg gttgaaccag gtgcacctgc gctacatgcc attgctgatc
    37081 actttggcgc taacatgatt gctgctgatg gaacattgca gcgccgggcc ttgcgcgagc
    37141 ggatcttcgc caacccggaa gagaaaaact ggcttaacgc cctgctgcat ccgctgattc
    37201 agcaagagac gcaacaccag atccagcaag ctacttcccc ctatgtactg tgggttgtgc
    37261 cattgctggt agaaaactca ctgtataaaa aagcgaatcg agtgctggtg gtggatgtca
    37321 gcccagaaac gcaacttaag cgcaccatgc agcgcgatga tgtaactcgc gagcatgtcg
    37381 aacaaatcct tgctgctcag gcaacgcgcg aagcccgcct tgccgtggca gatgacgtca
    37441 ttgataataa cggcgcaccg gatgctatcg catcggatgt tgcccgcctg cacgcacact
    37501 atttgcagct tgcgtcgcag tttgtctcac aggaaaaacc gtaatgcaga cccaggtcct
    37561 ttttgaacat ccactaaatg aaaaaatgcg tacatggctg cgcattgagt ttttgattca
    37621 gcaactcacc gttaatttac ccatcgttga ccacgctggc gcgctgcatt tcttccgtaa
    37681 tgtcagtgaa ttactggatg ttttcgagcg cggcgaagtc cgcactgagc tgttgaaaga
    37741 acttgaccgg cagcaacgta aactccagac ctggattggc gtgcctggcg tggaccagag
    37801 ccgtattgaa gcattaattc agcagttaaa agcggcgggg agcgtattaa tttccgcgcc
    37861 gcgtatcggg caatttctgc gtgaagatcg tttgattgct ctggtgcgtc agcgactgag
    37921 catcccaggc ggctgttgca gctttgattt acctacattg cacatttggc tgcatctacc
    37981 ccaggcgcag cgcgacagcc aggtagaaac ctggattgcc agcctgaacc cgctcaccca
    38041 ggcacttacc atggtgctgg atttaattcg ccagtcggcc cccttccgta aacaaaccag
    38101 tctgaatggt ttttatcagg ataacggtgg cgatgccgac ttgctgcgcc tgaatctgtc
    38161 gctcgattca cagctttatc cgcaaatttc cggacataag agccgttttg ccattcgttt
    38221 tatgccgctg gacactgaaa acggacaggt accggaacgt ctggatttcg aactggcctg
    38281 ttgctaagga gtaaaaatgt cagaaactat tacggtgaat tgcccaacct gcgggaaaac
    38341 ggtggtgtgg ggtgaaatca gcccgtttcg gccattttgc tccaaacgtt gtcagctgat
    38401 cgacctcgga gaatgggctg ctgaagaaaa acgaatcccc agcagtggcg atctttccga
    38461 aagcgatgac tggagcgaag agccaaagca gtgacatttg tctgatgccg cacgtaggcc
    38521 tgataagacg cggacagcgt cgcatcaggc atcttgtgca aatgtcggat gcggcgtgaa
    38581 cgccttatcc gacctacaga cgcttaagct tcgcaattac cggttcattg gctggcggaa
    38641 aatcatcggc attaagaccg actagcgaca tccactcacc gggttgccct tctttacccc
    38701 acggctcccc ttcccagctt tcgaccagcc agaaccacag agttatatgc ctgtccggga
    38761 attcatattc cagtttttca aatagcgaaa aatgttgtgg ggtaatcccg acttcttcct
    38821 gaagttcacg cactaccgcc tgttccggcg tttcgcccat ttcaatttta ccgccgggaa
    38881 actccagttt attcgccatg tgcgcatctg ctgcgcgacg cgttataaag atttcattgt
    38941 tctcgttgcg aataatacct accgcaattt gcagcttttt cattgtctta aacctataaa
    39001 aaaggcgcag aatcctgcgc cttttacttc aactgttagc ttttattgca ggcgaccatg
    39061 gcactgcttg tattttttac cagaaccgca cgggcaagga tcgttacgtc ctactttgcg
    39121 ctcaccggtt tgcgccgcca gtgcagctgc ggctgcagag tcgtcatcct gatggctaag
    39181 ctgctgcatt tgcgctaaac gctcggcttc catacgacgc tgttgttcca gctcctcaac
    39241 ctcttcaggc atacgtacct ggactttgct cagcgtgctg ataacttcat attttaacga
    39301 ctccagcatc gctgcaaaca tggagaacga ttcacgtttg tattcctgct tcggatcttt
    39361 ctgtgcatag ccacgcaggt ggataccctg acgcagatag tccatcgccg ctaggtgctc
    39421 tttccacaga gagtcgagag tttgcagcat gacgcctttt tcgaagtgac gcatcatctc
    39481 agcaccaacc acttcttctt tacgctgata cacttcgatg gactgcgcca gaattcgctc
    39541 acgcagcgtc tcttcatgca gttctggttc tttatccagc cactcggcaa ttggcaaatc
    39601 gaggtcgaaa tcgttcttca gacgttcctg cagccccgga atatcccaca tttcttccag
    39661 tgactgtggc ggaatgtagg catcaatggt cgctttgaac acatcttcac gaatgctgtt
    39721 gatggtttcg ctcacatcgc tgacatccag cagttcgtta cgctgggagt aaatggcgcg
    39781 acgctgatcg ttagccacgt catcatattc cagcagttgc ttacgaatgt cgaagttacg
    39841 gctttcaact ttacgctggg cgttggcaat cgctttagtc acccacgggt gttcaatggc
    39901 ttcgcctggc ttcataccca gtttacgcat catgccggat actcggtcgg aagcaaaaat
    39961 acgcatcagc gcatcttcca tcgacaggta gaaacgggaa gaaccagcat ccccctgacg
    40021 accagaacga ccgcgcaact ggttatcgat acgacgggat tcgtgacgct cggtaccgat
    40081 gatatgcagg ccacctgctt ccagtaccgc atcgtgacgt acctgccagt cggctttaat
    40141 tttttcaatt tgctctgcgg tcggattttc cagcgcggca acttctgcct gccagctacc
    40201 accgagcaca atatctgtac cacgacccgc catattggtc gcgatagtca ccgcagccgg
    40261 ataacctgcc tgagcaacaa tcgccgcttc gttggcgtgg aatttggcgt tcaggacgtt
    40321 gtgcttaata ccggctttgg tcagttcgtt tgacaccagc tccgattttt cgatggagat
    40381 agtacccacc agcaccggct ggcctttcgc agtacgttct ttgatatctt caatgatcgc
    40441 ctgaattttt tccgcttcag tcatgtagac caggtccggc agatctttac gaatcattgg
    40501 acggttggtc ggaacaacga cggtatccag cttgtagatg gagctaaatt cgaaagcttc
    40561 ggtatcagca gtaccggtca tccccgccag tttttcatac agacggaagt agttctggaa
    40621 ggtgatcgaa gccagcgttt ggttttcgtt ctggatctgc acaccttctt tcgcttccac
    40681 agcctggtgc agaccatcgg accagcgacg gccctgcatg gtacgaccgg tgtgttcgtc
    40741 aacgatgata acttcaccat ctttaacgat gtagtcgacg tcgcgggtaa acagcgcatg
    40801 agcgcgcagc gccgccgtta cgtggtgcat cagcatgatg ttggccggag agtacagaga
    40861 ctccccttca tccatgatgc cctctttcac cagcagttct tcaatcagca ccagaccacg
    40921 ttcggtcagg ttcacctggc gagatttttc gtccaccgag aagtggcctt cgccctggaa
    40981 ggtttcggag tcttcttttt cctgacggat caggtgcgga ataattttat tcacgcgttt
    41041 atacatttcc gagctgtctt ctgccgggcc ggaaatgatc agcggtgtac gcgcttcatc
    41101 gatcaggatg gagtccactt cgtccaccag cgcatagtgc agtttacgct gtacacgttc
    41161 ttcagggctg aacgccatat tgtcgcgcag gtagtcaaag ccgtattcgt tgttcgtacc
    41221 gtaagtgatg tcagctgcgt aagcttcgcg ctttgccggt gctggcatgc ccggcaggtt
    41281 gataccgaca gtcaggccaa ggaattcaaa cagcggacgg ttgttttcgg cgtcacgttg
    41341 cgccaggtag tcgttgacgg taactacgtg cacgccttta ccggttagtg cgttcaggta
    41401 agcaggcagc gttgcggtca gggttttacc ttcaccggta cgcatttcgg cgatgcagcg
    41461 ttcgttaaga accataccgc cgagtaactg aacgtcgaag tgacgcatac caaagacgcg
    41521 cttacttgcc tcacgtacca cggcgaaagc ttccgggatc agattttcca gcacttcgcc
    41581 tttttccaga cgtgcacgaa actctgcggt tttccctttc agttcttcgt cggagagttt
    41641 ttccatctcc ggttccatgg cattgatgat gttgaccact ttgcgcatcc ggcgcagggt
    41701 gcgatcgtta cgactaccga aaactttagt taacaatttg attagcataa taaaatctca
    41761 aacgccccgc gttgcggagt taataaaatg aagtaaaggt ttattgttgt taggtgaggc
    41821 gttgagggcc agcacggatg ccttgcgcct ggcttatcca gacgggcgtg ctgaattttg
    41881 cttgtggggt aaaatgcgca taatcaatgc gataaccctt ttcagacggc gtgccttcct
    41941 gggtcagcag cgcgctgagc gtatccagta atgcaagatg ttgcgcctga agaggcaaag
    42001 actcttcagc aacgggcagt gtttgcggtg ccattgcgaa agaaagatga cggattaccg
    42061 tgcgaatggc atgttgatgc cagtaatcaa cggaatagtt cgaattcggg cggcgtgtgt
    42121 tcgcttccag caaggccaat tgaccaaagt taactttggc tgaaggctcg tggttgcggg
    42181 ttgtcgcttt tgcgggcgcg tttggttcgg cggcgttgct gagcgcaggc aaacctaaac
    42241 tcgccgcaac catccctaat aagagatgcg gccagaagta gcgtttacca aactgtcgcc
    42301 agcgcgtcag tattccactc acgttattgc catcccgatc gtattcaaac gtatttagcg
    42361 cacaaatctt atcattaaag cctgcagtcc acagcacgaa tgaagataag ttgtgcggaa
    42421 aagtgcttaa gaaaacagcg ttcgcaccgt tttttatagc tcttataaaa ttcaaaaaaa
    42481 aatgactggt tcacctggcc ggagagagtg ccagatttgc cagtcgaatt ttatacgaca
    42541 gtataaatgt cgttatgcca gtacagctga aggcgctttg aaggccaacg gcagttctgc
    42601 gtcgtcctgg aaggtcacat attcccaggc ttcctgtttc gccaggacag cctgcagcag
    42661 tttgttattc agtgcatgac cggatttata agcggtaaat gcaccaataa tattgtgacc
    42721 acacatgaac aagtcaccga tcgcatcgag cattttgtga cgcacaaatt cgtcttcaaa
    42781 acgcaggccg tcttcgttca gtacgcgata atcgtcaaca acgatggcac aatcgaagct
    42841 gccgcccagg cacaaaccac gggactgcag atattcgata tcacgcatga aaccgaacgt
    42901 acgcgcacgg ctgatctggc gcataaacgc atcagcggag aagttcatcg catagcgctg
    42961 gttgctggaa tcaatagccg gatggttaaa atcgatggtg aaatccagcg aaaaaccatt
    43021 gtacggctta aattcagccc acttatcgcc atcttcgaca cgaacagtct ctttgatgcg
    43081 aacaaatttt ttggcgcagt tcaactcgtc gataccggcg tcaagcagca ggtatacaaa
    43141 cggagcggcg ctgccgtcca tgatcgggat ttccggcgcg ttaacttcga taacaatgtt
    43201 atcgatgccc aggcccgcga gagcagcatt gaggtgctct acggttgaaa tccgtacatc
    43261 atgctcgttg accagacacg tacagagcat ggtatcacgc acagatttgg catcggccgg
    43321 gaaatctacc ggtggattca agtcggtgcg acgatagatg accccggtgt tggccggcgc
    43381 agggcgtaac gtcagagtga ctttcttgcc ggtatgtaaa ccgacacccg tcgcctgaac
    43441 gatacgttta agtgtccttt gtttgatcat cgtattatct cgccaaatta cctatccaac
    43501 cgaagtgtac tatacattcg gcgggccagt ttagcacaaa gagcctcgaa acccaaattc
    43561 cagtcaattc ttaatcagct tgcttacgca ggaatgctgg gatatccaga taatccggct
    43621 ctttcgcagt ttgcggcgca ttgtcattca cgactttagc aaccggcttc tgctcctggg
    43681 tcagcggagc catcccatgc tgctggtagc gatccatcac tggctgctga acctgcttat
    43741 tggtcaccag agtgatttca ggacgtttgt ccatgccgat acctgtcgca acaacggtta
    43801 cgcgcagctc gtcattcata tccgggtcaa gagaagtacc gataaccaca gtcgcgttgt
    43861 cggaagcaaa tgcacggatg gtgttaccta ccgtttcgaa ctcatccaga cgcaggtcga
    43921 agcccgccgt gatgttaacc agcacgccgc gcgcgccaga caggtcgata tcttccagca
    43981 gcggagaaga gatagccatt tcagcagctt cttccgcacg gtcttcaccg ctcgccacgc
    44041 cagaacccat cattgcgtag cccatctcag acattacggt gcgtacgtct gcaaagtcca
    44101 cgttcatcaa acccggacga gtaatcagtt cagcgatacc ttgcacagcg cctttcagta
    44161 catcgttcgc tgcgccaaac gcatccagca gggagatacc gcggcccaga actttcagca
    44221 gtttgtcgtt cgggatagtg atcagagagt ccacatgctt ggacagttca gtgatcccct
    44281 gctccgcgaa tgccatacgc ttcttgcctt caaagttgaa aggcttagtg acgacagcaa
    44341 cggtcaggat acccaaatct tttgccactt cagcgacgac tggtgctgca cctgtaccgg
    44401 taccaccacc catacccgca gcaataaaga ccatgtctgc accttccagc gccgcacgca
    44461 atgcatcgcg atcctcatca gccgcattgc gaccaacttc tggattagcg ccagcgccca
    44521 gtcctttggt gataccgcta ccgatttgaa tcgtctgtcc aaccgctgtt ttacgcagcg
    44581 cttgtgcatc ggtatttacc gcgaagaatt caacaccttc aatacgctcg cgcaccatgt
    44641 gttcaacagc attaccgccg ccgccgccga cgccgatgac tttaatcacc gcgtcattgg
    44701 taagttccat tggttcaaac atagtttctc tccgatttgt gcctgtcgcc tgaggccgta
    44761 atcatcgtcg gcctcataat aattaaaact cttttcgcag ccaactattg agtcgcttga
    44821 tccacgagcc aactgatgct gtaacacgtt tttctacttc agcttcaccg ttaagatgtg
    44881 actctttccc atagtgaagc aatcccaccg ccgtcgaata atacggctcc tgagcataat
    44941 ccgttaaacc ggtaatgttc agcggcgcgc cgatacgcac ttgcgtatga aacacgcgct
    45001 gagcacaggc tgcaagacct tcgatctgcg ctgcgccacc ggttaataca atgcctgccg
    45061 ccaggtgatg tttaacccct tgttggcgaa gcttttcctg caactgcaat atctcttcgt
    45121 tgaccaggtt gagcagctcg gtatagcgcg gctcgatcac ctctgccagg gtctgacgtt
    45181 gcagactccg tggcggacga ccgcctacgc tcggcacttc cacgctctca tcttttccaa
    45241 cgatggaacc cagcgcacaa ccgtggcgaa ctttaatcgc ttcggcgtcg cttggcggcg
    45301 tgccaaaggc gtaagcgata tcactggtca cgacattgcc agcataagga attaccttag
    45361 tgtggcgcaa tgccccaccg gtataaacgg cgatatccat tgtaccacca ccgatatcga
    45421 cgacgcagac acccagttca cgttcatctt ccgtcaatac cgaataactt gatgccagtc
    45481 cggcaaatat cagttggtca actttcagcc cacaacgttc aaccgctttg acgatgtttt
    45541 tcgccatatc gttgtgacat gtgatcaggt gcacttttgc ctgcatccgc acgcccgaaa
    45601 gtcctaccgg attcttgatc ccttcctgat agtcaatcgc atactcttgc gggatcacat
    45661 gcagcacacg atgttcatcg cgcacacgca ccgatttcgc ggtatggacg acgttttcca
    45721 catcttcttg cgtcacttct tcttcagaaa taggcaccat accaatttca ttctggcagc
    45781 tgatgtgctt accagaaagc gccagatata ccgaagagat ctgacaatct gccatcaatt
    45841 ctgcctggtc aatggcgcgt tgtacgcact tgaccacgga ttcgaggtcg ttcaccccgc
    45901 ctttatccat accacgtgac gggcagctgc ccacgccaat gatattgacc ataccgtcgg
    45961 gcagaacttc ccctactaaa gcggcaacct tcgcggtacc aatctccagt cctactacca
    46021 gttttctgtc cgtcgccttg atcattgttg ttctgcctgt gcctgatttt gttgctgagt
    46081 agattcctct ggcggcaagg gcgcccagcc tactgccgct ccagagtcat aacgcaaatc
    46141 aacgtagcta atccgtttgc catcggtttg cgcctgctgc tgtaaaaccg gataaagttc
    46201 tacaaagcga gccaaacgtt tcatcgtatc gccccggcca agattgagct taatatcgtt
    46261 attcagcgtc aactgccagg aacgccgcgc ggtcatcgcc gcttccttca gagtaaatct
    46321 gtcctttgcc agcatctgcc ccatttcgcg atagccctgc aacacttcat tggcgctgcc
    46381 ttccgggcca tacagcattg gaagcacctg cttgctggtg cgatctggcg gcacgctgaa
    46441 ggtgtttcct tccgcgtcta ccatatgttg atcattccac cgcgcaatcg gcacatattc
    46501 aaccagatga atcttcaatt catcaggcca ctgctttctg acgctcacct gcttaatcca
    46561 cggcaggcgt tgttctattt gcgtctggat gatgttgaca tcctgggtca taaaggtacc
    46621 cggctcaccc aatgccagga tcgactgccg gatatcgtca ttacgcgtgt aatggcgttc
    46681 accggtcaac accagctttg agagcggcag gcgttgcgca tcttccatcc agcccaacac
    46741 gacccagccg ctcaccaaca ctgtcgttaa aacggtcagc aggaaaagga tccccgccag
    46801 acgcgttcca ttattgcggc gagaagaaac ctcttcttcg ctatttcgcg tgttcagagc
    46861 agcctgcgac atattagtcc gccagttcca gaattcgtac taccaactgc gagaagctca
    46921 tacctgcctg acgtgccgcc atcggcacca ggctgtggct ggtcataccc ggtgaggtat
    46981 tggcttccag cagataaaac tgtccatcgc tgtccagcat aacgtcaata cgtccccatc
    47041 ctttgcaacc taacgtcgtc catgctttca gcactaatgc ctgcaaattg gcctcttgtg
    47101 acgcttccag acctgcgggg cagaaatact gtgtctcatc agagagatac ttcgcctcat
    47161 aatcatagaa ggttccggac ggttgaatac gtattgacgg taaaatttct tcaccgagta
    47221 tcgcaaccgt gaactccggc ccacttagcc atttttcaat caatacttct tcatcgtgct
    47281 gaaatgccaa tcttaatgca tcttgtagag cattttctgc tactactttt gacattccca
    47341 cactggaacc ttcgcggctc ggcttaacga taaccggcaa acccagagca gaaatttctg
    47401 ctaactgctt atcgctcagg cctttttcaa actctgcgcg ggttaacgct acccacggcg
    47461 cgaccggtaa accggcacct tgccacagca gtttgctgcg tagtttatcc attgaaagcg
    47521 cagatgccat cactccgctg ccggtataag gcaagcccat cagctcaagc atgccctgca
    47581 gcgtaccatc ttcaccgccg cgaccgtgta gcgcgataaa cactttctga aaacccatcg
    47641 acttcagttg cgtcacgtcg acttctttcg ggtcgacagg atacgcatca ataccgcctt
    47701 cacgcagccc ggctaacacc gctgcgccag aattcagaga aacttcccgc tcagcggaag
    47761 tcccgcccaa caggaccgca attttatcag tcatgttgtt cttcctccgg agtttgcggc
    47821 ttcagtttga tttcagctaa agaacgggca atttttccga tattaccagc cccctgaacg
    47881 agaatcaggt cgttaccgat taataccggt gccagcatct cggctacctg cgcagggtcc
    47941 ggcaccagaa tgggatcaat tttcccacgt ccacgaattg tgcgacacag cgaacggctg
    48001 tccgctcccg gaattggcgc ttcgccagcc ggatacactt ccagcatcaa cagggtatca
    48061 acctgcgtca gcacattggc gaaatcatca tacaggtcgc gcgtacgggt aaaacggtgc
    48121 ggctgaaaca gcattactag gtttttatcc ggccagcctg cgcgcgccgc tttaatggtg
    48181 gcgtcaactt ccgtcgggtg gtggccgtag tcatcgacca gcattgccgt accgctttta
    48241 ccattcactg gctccagcgg gaattcaccg aggaaatcaa aacggcgacc agtcccctgg
    48301 aagctctcca gcgcacgcaa aatagcctcg tcgtcaattc cctcttccgt agcaaccgca
    48361 accgcagctg cggcgttcag cgcgttatga cggcctggcg cattcagggt gacgcgcatc
    48421 ggctctttgt cctggcgcag cagcgtaaag tgcccctgcg ggccaatctg ctgataatct
    48481 tctacacgca cgtcggcatc ttcgctgaag ccgtaagtcg tggtctgacg tcccacacgc
    48541 ggtaacaatt cgcggatcac cggatcatca acacacatca ccgcacgacc gtaaaacggc
    48601 aggttgtgca gaaaattaat aaaagtctgt tttaaattct caaagtcgcc ctggtaggta
    48661 tccatgtggt cggcttcgat attggtgaca atcgccacca tcggttgcag atgcaggaac
    48721 gatgcatcac tctcatctgc ttcggcaatc aggtaccgac catgccccaa acgcgcatga
    48781 acccccgcag cttttaccag cccgccgtta acgaaggttg ggtcgagccc cgcttctgcg
    48841 tagatgctgg aaaccatcgc ggtggttgtc gttttgccgt gcgttcctgc aatggcgatg
    48901 ccatgacgaa aacgcattaa ctcagccagc atttcggcac gacggatcac cggaatacgc
    48961 gcttcatgag ctgcgacaat ttccgggtta tcggcagaaa tggcgctgga aacaacgacc
    49021 acgctggcat cacgtacgtt ttccgggcga tggttgaaat aaatcgtcgc tcccagattc
    49081 attaactgct gcgtgaccgg atttggcgct aaatcggaac cactgatctg ataaccttca
    49141 ttggccagaa cttcggcaat accgcccata ccggcaccac cgatgccgac aaaatgtatg
    49201 tgccgaacgc gacgcatttc gggcacgatg gaacgcagtt ttgccaattg ttgtgtattc
    49261 attctttacg ccattaactt cttaaattca tacgatgcaa aaggcatcgc tacaattacg
    49321 cccgggcagc ccggctcact tcatttgcca ctcgctcggt ggcatccgga atggatgcag
    49381 cgcgggcgcg ttctgccatg gttaataagg tttctcgcga ccacccggcc agggtgttgg
    49441 cgacagcatc cacgctaagc tgtggctgct cgataatttt ggctgcgccc gctttttcca
    49501 gcggtagcgc attccagtat tgctggcggt ctttatgttg aaacggcaca aacaacgccg
    49561 gtagtcctgc cgcggcgatt tcactcaccg ttaaagcacc ggagcggcaa acgacgacat
    49621 ccgcccacgc atacgccgcc gccatatcat caataaattc cgtcacttta tgctgcggtt
    49681 gccccgcttc ggcatacgcc tgttcaacgg attgttgcga acctttaccg ctctgatgcc
    49741 agatagtgac tgaatcaccc agtttcgcag caacctgcgg cattgtctgg ttaagaatgc
    49801 gtgcgccctg ggaaccaccc actaccagca cacgaaccgg accttcacgt ccagccaaac
    49861 gttgctgcgg caacggcagc gccagcacat cggtacgcac cgggttaccc accacttccg
    49921 cattagggaa agcgcctgga aacgcctgca tcactttggt ggcaatcttc gccagccatt
    49981 tattggttaa gcccgcaata ccgttttgtt catgaagtac aaccggaatg cctaacgacc
    50041 acgcggccag accacctgga cctgatacat agccgcccat accgagtacc acgtcaggtt
    50101 tgtacgcttt cataatcgcc cgcgcctgac gccaggcgtt gaagatacgc agcggcgcag
    50161 ctatcagtgc ttttatacct tttccacgca gaccagagat acgaatgaaa tcaatttcga
    50221 tgccatgttt tggcactaag tccgcttcca tacggtcggc agtccccagc cagcgaactt
    50281 gccaaccctg agccattaga tggtgcgcaa ccgccagtcc cgggaataca tgtccaccgg
    50341 ttccgcctgc catcaccatt aatcgctttc cttgaccact catcgtgaac ctcgtacaaa
    50401 cgcctgcgct ttctccagac gcgtttcata atcaatacgc aacagcatca tgatggctgt
    50461 cgacataatc agtaagctcg aaccaccgta actgatcagc ggcaatgtca gacctttggt
    50521 cggtaacatc cccgccgccg cgcctacgtt aaccagcgcc tggaagctaa accagatgcc
    50581 aatagaacag gcgagaaaac cggaaaaacg gtggtcaatt tctaacgctt tgcggccaat
    50641 cgacatcgcg cgaaaagcga cgaagaatac cattaaaagt gccagcacca caccgacata
    50701 ccccagttct tcgccgataa tggcgaaaat aaagtcagtg tgcgcttccg gcagatactc
    50761 cagtttttgt accgagttac ctaaaccttg tccccaaagt tcgccgcgac caaacgccat
    50821 cagcgattgc gttaactgat agccgctgcc aaagggatct tcccacgggt tccagaatgc
    50881 ggtaacacgg cggatacggt acggttcggc gagtatcagc aacacaaccg ctgaaatgcc
    50941 cataccgata atggcaatga actgccacaa tttcgctccc gccaggaaca acatcgccag
    51001 cgtagtcaca aacaacacca ccaccgtacc aaggtctggc tgtgccagca gtaacactgc
    51061 caacaccaga atcacgccca tcggtttcag gaagccgcgc aggttattac gtacttcgtc
    51121 gcctttacgc accagatagt tggcgatata acaaaacagc gacagttttg tcagctccgc
    51181 aggctggata cgcagcaaac cgagatcgat ccaacgcgat gcccctttaa ccgagctacc
    51241 cactaccagc acgatcatca gcaggatgat agatccgagc agcatcgtgg cactgtagcg
    51301 ttgccagaac tccatcggca gacgcagcgt aatgatcgcc agaatgaatg ccaggatcag
    51361 atagacgcca tcacgcttcg cgaagaagaa cggatcgttg gttaagcgtt gccctatggg
    51421 cattgacgcc gaggtcacca tgataaagcc aatcgccgca aggccgaagg tcagccacag
    51481 taaggtgcga tcgtacatga tcaggctgtc ggtatctttt tcccgcgagc ccatcaccca
    51541 gccttttagc gccgtggaga tccagaccag gatactgaat cctggcaggc gcggcatttt
    51601 caggcgaggg agagataaac gcatcaacct aactccttcg ccagacgggc aaactcattg
    51661 cctcgttgtt caaagttctt gaactgatca aggctggcac aggccgggga gagcagaacc
    51721 atatcgccca gctgaacacg cggagccagc aagcgcatcg cttgttccat ggtttcggtt
    51781 tgttctgcca cttccgggcg tagcgccgcc agctgcgcac cgtcacgacc aaaacagtac
    51841 aaacgcacgt tgtcgccatt cagatagcgc gccagtggac tgaagtccgc cgatttacca
    51901 tcgccgccca gcaacaaatg cagcgtgccg tctacctgca agccattcaa cgccgcttcc
    51961 gtactgccga cgttagtggc tttcgaatcg ttaatccagc gtacgccatt gtgctccagc
    52021 accacttcaa agcgatgcgg cagaccggtg aaggtggtta acgctttcag gctgctggcg
    52081 cgcggcaaac cagctgcgtc tgccagtgcc agtgctgcca gcgcattggt gtagttatgc
    52141 tgcccggaaa gtttcatctc tttcacattc agcactttct cgcctttaac ccgcagccag
    52201 gtttcgccct gctgatgatt caggtgatag tcacccatgt tgacgccaaa gctgacgcag
    52261 cgttcatcag caccgcgaat cggcattgtt aaggcatcat cagcattgac gacacaaact
    52321 ttcgcatttt cgtaaatacg caattttgcc gcacggtatt gctgcaaacc aaacggatag
    52381 cggtccatat gatcttcagt cacgttcaga atggtcgccg ccaccgcctg taagctggaa
    52441 gtggtttcca gctggaagct cgacagttcc agcacgtaca gttcgcactc agcatcaagc
    52501 agcatcaacg ctggcaggcc aatattgcca cccacaccaa cgttaacccc cgccgctttc
    52561 gccatttcac ccactagcgt ggtgaccgtg cttttgccgt tagaaccggt aatcgccaca
    52621 atcggtgctt gtgcttcgcg acagaacagc tcgatatcgc caacgatttc gattccggca
    52681 tcagcggcag cgcttaagga tggatgcgcc tgtgcaatac cgggactggc gacaatcaga
    52741 tctgccgcca tcagccattc atcattcaga ctgcccgtgt ggcgttctac ggcttcgggt
    52801 aatttatcca ggccaggcgg tgtcatacgc gtatccataa cgcgcggcgt cacaccgcga
    52861 gcgaggaaaa agtccacgca ggaaagcccg gtgaggccca ggccgataat gacgacattt
    52921 ttaccctgat aatcagccat gattaacgta ccttcagcgt tgccagacca atcagaacca
    52981 gcatcagcga aataatccag aaacgcacaa tgacgcgcgg ttccggccag cctttcagtt
    53041 catagtggtg atgaatcggt gccatgcgga aaatacgttg tccgcgcagt ttaaaggagc
    53101 cgacctgcag gatgacagaa agcgtttcta ccacgaacac gccccccata atcaccagca
    53161 ggaattcctg acgtagcagt acggcgataa tgcctaacgc accacctaac gccagcgaac
    53221 ctacatcgcc cataaagacc tgcgccggat aggtgttaaa ccacaggaag cccagtcctg
    53281 ccccgactat cgccgtgcag acaatgacca gttcccctgc gtgtcgcaga tacggtatat
    53341 gcaagtagct ggcaaagttc atattgccgg tcgcccacgc caccagcgca aaaccaccgg
    53401 cgacaaatac ggtcggcata attgccaggc cgtcgagacc atcggtcagg tttaccgcgt
    53461 tgccagtacc cacaatgacg aagtaagcca gcagaatgta gaacagcccc agctgcggca
    53521 tcacatcttt aaagaatggg accaccagct gcgttgcggg cgtgtctttg ccggcaaggt
    53581 acagggcgaa ggcgacaccc agcgcaatga ccgacatcca gaaatacttc caacgagcga
    53641 tcaacccttt ggtgtcttta cgcaccactt tgcgataatc atcaacaaag ccaataacac
    53701 cgtaacctac cagcaccacc aacacgcacc agacgtacgg attggacggg taagcccaca
    53761 gcagtacgga gatcacaatc gccgtcagga tcataatccc gcccatggtc ggcgtaccgc
    53821 gcttgctgaa gtgtgattca ggaccgtcgt tacgcaccac ctgaccaaag gaaagttttt
    53881 gcaaatgagc tatcatacgc gggcccatcc acaatgagat gaacagcgcg gtcagcaggc
    53941 tgacgatggc gcgaaacgtc agataggaaa agacgttaaa gccggaataa tatttgacca
    54001 aatgttcggc cagccaaact aacatgtccc attctcctgt aaagcgcgta ctacctcttc
    54061 catggcggca ctacgtgaac ccttaactaa aatcgtaatt acctgttgct cagcaatcag
    54121 tgatttaaga cgcgtaatta acgcagtttt atcagcaaaa tgttcgccaa cgccgctggc
    54181 ggtgctgata gcatggcttt gtttacccac gcttaacacg cggtcaatac cagcagcttt
    54241 tgccgcctcg cccacctgta catggcaggc ttcgctttca gcgcccagtt ccgccatatc
    54301 gcccaccacc agcacgcggt agcccggcat ttcagccagt acctgaacag cggcggtcat
    54361 tgaaccgaca ttggcgttgt aggagtcgtc gagcagcaac tggttttctg ccagttggat
    54421 ggggaacaga cggcctggaa cagctttcag atttgccagc cccgctttga tagcatcaag
    54481 cgttgcgccc acggacatgg agagcgcagc ggctgccagc gcattcgcaa tattgtgacg
    54541 ccccggcaac ggcagcagaa catcgacgct accggttggg gtttgtaggg taaattccgt
    54601 accgtgcgag gtcacatgga tattggtggc ggtgaaatcg ctgttggcgg cattgggtga
    54661 gaaacgccac actttgcgtg agccaattac gctctgccag ttcagccagt cgttgttgtc
    54721 ggcgttcata atggcgatac cgttttccgg caggccgcta aagatttcac ctttcgcttt
    54781 cgcgacaccc gcaagcgagc caaaaccttc cagatgcgcc gctgccaggt tgttgaccag
    54841 cgcagcttcc gggcgagtca gactcacagt ccaggctatt tcgccctgat ggttcgcgcc
    54901 aagttcaata actgcgtaat cgtattccgg cgtcaagcgc aacaacgtca tcggtacacc
    54961 gatgtcgttg ttgagattgc ctgccgtata aagcgtgttg ccgcactggc ttaaaatcgc
    55021 cgccgtcatc tctttaacgg aggttttgcc ggaggaacct gtcagagcaa ccacgcgcgc
    55081 cggaacttgc tggcgaaccc atgcagccag ttcaccaaac gccagacgcg tatccttgac
    55141 gattaactgc ggcaggtcga tatccagcgg acggctgacc agtagtgcgc ccgcgccgcc
    55201 agctttcgcc tggtcggcaa aatcatgagc atcgaaacgt tcgcctttca gggcaacaaa
    55261 caggcagccc ggcgtcagtt ttcgggtatc agtggttaca gcatcaaggg tgatatctgc
    55321 accttgcagt tcaccgttga gaatgtcggt aagttggcta agggttacgc taatcatgca
    55381 atcaccccca gcagacgcgc caccgtgacg cgatcggagt agtccagacg ctgattgcca
    55441 acaatctggt aatcttcatg gcctttgccc gcgaccagta ccacatcatt ctctttagcc
    55501 tgcataacgg cgcaagtcac cgcttcagca cggccttcca tcactttggc atgtccggca
    55561 tctaacattc ccgccagaat atcgttgatg atggcacgcg gttcttcggt acgcgggtta
    55621 tcgtccgtca ccaccgccac gtcagcaaac tcttcggcaa ttgcgcccat cagtggacgc
    55681 ttacctttat cgcgatcgcc accacagcca aagacgcacc acagcttgcc cgcacagtgc
    55741 aggcgcgccg cctgtaaggc tttttccagt gcatccggcg tatgcgcgta atccaccacc
    55801 accgtcggtt tgcctggcgc agtgaacact tccatacgtc cgcaaaccgg ttgcagacgc
    55861 gcggcggttt tcagcagatc agccagtgga tagccgagtg ccagcagtgt cgccagcgcg
    55921 agcagcaggt tgctgacgtt aaaagcgccc atcagatggc tttcaatttc gccatcgccc
    55981 caacttgagc taaagcgaat cgtcgcaccg ctgtcgtgat agttcacgtc gatcgctttt
    56041 aaccagcgtc cgtgacagtt cggattaata tgatcttcca ttgataccgc aaccgcgtcc
    56101 ggcagttttg ccagccagcg gcggcccact tcatcgtcgg cgttaataat cgcctgaccg
    56161 caatgatgtt cagaataaag cagccatttc gcagcttcgt agtgttccat atcaccatga
    56221 taatcaaggt gatcgcggct taagttggta aagaccgacg ccgcaaattt caatgccgcc
    56281 acacggtgct gtaccagccc gtgggaggaa acttccattg cgcaaaacgt cgcgccctgc
    56341 tccaccagcc ccgccagctc atgctgaaca tcgactgcgg aaccggtagt attttctgtc
    56401 gggatcactt tcccaagcag accgttacca acggtgccca ttaccgcgct ggtttcgcca
    56461 agcagttggc tccactgcgc cagcagctgg gtagtcgtgg ttttgccgtt ggtgcccgtt
    56521 acgcctacga gacgtaaatt gtcagagggt tcatggtaaa agcggcccgc cagtgcagat
    56581 aaacgctcat tgagctggct gagatagatg accggtacgc cgtgcatttc acggatttca
    56641 ccgtcggtcg cctcatcttt cgctttcgcc tctgcaataa tggcagccac accttgcgct
    56701 atcgcctgcg ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga
    56761 tcgcccgccg cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg
    56821 tctggcaccc acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc
    56881 ttgattaatc acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat
    56941 ggtacgcaat acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta
    57001 gtatttaccc gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc
    57061 aggcgcaacg cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac
    57121 ctttttcgcg gtaccggttt taatggcgat acgatagcct ttaatcgccg ccttcacgcc
    57181 gccgccgcct ggtagcgcca cgctttccat catatgcacc acagtgcgga caatggatcc
    57241 cgggaagaca cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata
    57301 aatgccgtag ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag
    57361 cccgtagccg aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata
    57421 taagccactg cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga
    57481 gtaagtatct actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt
    57541 actcgacttc tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat
    57601 ttcgtggccg ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc
    57661 acgttgcaac gcggtcatta ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc
    57721 ggtgatggta cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta
    57781 tgacgggctg ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct
    57841 accagattca gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg
    57901 caggcgttca tcaatactca gcgccaggtt gtgcgctgcc tggctgtcag tagaagaaat
    57961 atcttcaatt acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt
    58021 aagccattta tcgaaactct tctcaacgcc ctcaatccct tgactatcga cgttagtaaa
    58081 gccgatgagg tgagcagtca cttcgccgga cggatagtaa cggcgagact cttcacgcag
    58141 atgaatcccc ggcagtttca gttttttgat gtagtccgcc atgtcagggt tcacctgacg
    58201 cgccagataa ataaaacgcc ctttcgggtt ggcgttaatg cgggctgaaa gctgatccag
    58261 cggaatattg agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc
    58321 gtcatgcact tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg
    58381 gcgaccagaa cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga
    58441 acgcatgtcg ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg
    58501 tccgagcaga aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact
    58561 gataaagttg gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat
    58621 gcgtcgcgtt tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg
    58681 catttgcagc ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc
    58741 ttcaaggatc aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc
    58801 gcgctgagcg gtcagtaaac gggtatggtg cgccgtggtt accacagtca ccgccgtcaa
    58861 aataatgcaa atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat
    58921 aacaccaggc aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc
    58981 ttctgtcact ctgctgatca tgcattcgtc ctctctgcaa tacgcagaac tgaactacgg
    59041 gcacgagggt tctcagccac ctcttcttcg cccggcatta acttgcctag tgctcgcagc
    59101 tgacggccac ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc
    59161 ggaccgcggc tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag
    59221 ctgatgatcg aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc
    59281 tctatctcct ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgggtcgcg
    59341 ggatgtttaa acttatcttt caccggcgtt gcaacagcca cgacttccgc cagttctttg
    59401 gtgcgggtca tcggctgttc gcggttacgc tcgacaatgg cgcgggcaat gcgtttggca
    59461 aaacgctctt caccataggt tttcaatacc caagcgatat cggcttcttc tgcggtttgt
    59521 agccattcag cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg
    59581 cgcataaagg aaaagccgcg ctcagcatca tcaagttgcg gtgaagagac gccaagatcg
    59641 aggagaatgc cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg
    59701 gagaaaggtc cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata
    59761 gcctgcgggt cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc
    59821 agacgtgagt gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata
    59881 ttgaggccat taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt
    59941 ttatagagac aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc
    60001 gatatcttcc ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa
    60061 ctgtccaacc agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat
    60121 taacaatcga cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg
    60181 cacacggcgc tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc
    60241 ccattcaggc agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat
    60301 ttgaccgaca gcgttctcaa gcagctgttc ccggtaacgg gtaggcactg ataagcgccc
    60361 tttgctgtcg agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc
    60421 cactttgccc cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa
    60481 gccagtcaca aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt
    60541 ttattttacg tccaacgtgc gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc
    60601 ctcatcgtca tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca
    60661 gtttgcgacg cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt
    60721 ctgtgtggcg cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc
    60781 ggcaattcat ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat
    60841 tattgtgaat ttcagctggt gagttaatta acgggcgggt ttaaaagaac ttatcaataa
    60901 tagtcaggaa tagtcttatt tactttaagg atattgatgt ccattcccgt actctacg
//
LOCUS       DAEEKQ010000009        57432 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.009, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000009 DAEEKQ010000000
VERSION     DAEEKQ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 57432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.009"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            147..1490
                     /gene="argG"
                     /locus_tag="KLK57_000559"
     CDS             147..1490
                     /gene="argG"
                     /locus_tag="KLK57_000559"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HBE0891026.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENLENKGQ"
     gene            complement(1498..3105)
                     /locus_tag="KLK57_000560"
     CDS             complement(1498..3105)
                     /locus_tag="KLK57_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBE0891027.1"
                     /translation="MLEDFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFFVLTILV
                     VKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDPDEVVKM
                     LGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQFAYKDA
                     KNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVRDTGIDT
                     YVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQSISGAPYTALSVPLSLTAD
                     SVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETVYVRGFD
                     ELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDACYDNSIH
                     YTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHVPMFIWY
                     SPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPKTLEEVIAHYKGDSRVVDAN
                     HDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(3583..3669)
                     /locus_tag="KLK57_000561"
     tRNA            complement(3583..3669)
                     /locus_tag="KLK57_000561"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3633..3635),aa:Leu,seq:gag)
     gene            complement(3684..4016)
                     /gene="secG"
                     /locus_tag="KLK57_000562"
     CDS             complement(3684..4016)
                     /gene="secG"
                     /locus_tag="KLK57_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HBE0891028.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(4244..5581)
                     /gene="glmM"
                     /locus_tag="KLK57_000563"
     CDS             complement(4244..5581)
                     /gene="glmM"
                     /locus_tag="KLK57_000563"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HBE0891029.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGSRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(5574..6422)
                     /gene="folP"
                     /locus_tag="KLK57_000564"
     CDS             complement(5574..6422)
                     /gene="folP"
                     /locus_tag="KLK57_000564"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HBE0891030.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(6512..8446)
                     /gene="ftsH"
                     /locus_tag="KLK57_000565"
     CDS             complement(6512..8446)
                     /gene="ftsH"
                     /locus_tag="KLK57_000565"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HBE0891031.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(8546..9175)
                     /gene="rlmE"
                     /locus_tag="KLK57_000566"
     CDS             complement(8546..9175)
                     /gene="rlmE"
                     /locus_tag="KLK57_000566"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HBE0891032.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            9301..9594
                     /gene="yhbY"
                     /locus_tag="KLK57_000567"
     CDS             9301..9594
                     /gene="yhbY"
                     /locus_tag="KLK57_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HBE0891033.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(9750..10226)
                     /gene="greA"
                     /locus_tag="KLK57_000568"
     CDS             complement(9750..10226)
                     /gene="greA"
                     /locus_tag="KLK57_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HBE0891034.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            10474..11907
                     /gene="dacB"
                     /locus_tag="KLK57_000569"
     CDS             10474..11907
                     /gene="dacB"
                     /locus_tag="KLK57_000569"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HBE0891035.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYITQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAQKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(12093..13265)
                     /gene="cgtA"
                     /locus_tag="KLK57_000570"
     CDS             complement(12093..13265)
                     /gene="cgtA"
                     /locus_tag="KLK57_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HBE0891036.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFILE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(13281..14246)
                     /locus_tag="KLK57_000571"
     CDS             complement(13281..14246)
                     /locus_tag="KLK57_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBE0891037.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(14373..14630)
                     /gene="rpmA"
                     /locus_tag="KLK57_000572"
     CDS             complement(14373..14630)
                     /gene="rpmA"
                     /locus_tag="KLK57_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HBE0891038.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(14651..14962)
                     /gene="rplU"
                     /locus_tag="KLK57_000573"
     CDS             complement(14651..14962)
                     /gene="rplU"
                     /locus_tag="KLK57_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HBE0891039.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            15221..16192
                     /gene="ispB"
                     /locus_tag="KLK57_000574"
     CDS             15221..16192
                     /gene="ispB"
                     /locus_tag="KLK57_000574"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HBE0891040.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            16421..16699
                     /gene="sfsB"
                     /locus_tag="KLK57_000575"
     CDS             16421..16699
                     /gene="sfsB"
                     /locus_tag="KLK57_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HBE0891041.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(16747..18006)
                     /gene="murA"
                     /locus_tag="KLK57_000576"
     CDS             complement(16747..18006)
                     /gene="murA"
                     /locus_tag="KLK57_000576"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HBE0891042.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(18061..18315)
                     /gene="ibaG"
                     /locus_tag="KLK57_000577"
     CDS             complement(18061..18315)
                     /gene="ibaG"
                     /locus_tag="KLK57_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HBE0891043.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(18475..18768)
                     /gene="mlaB"
                     /locus_tag="KLK57_000578"
     CDS             complement(18475..18768)
                     /gene="mlaB"
                     /locus_tag="KLK57_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HBE0891044.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(18768..19403)
                     /gene="mlaC"
                     /locus_tag="KLK57_000579"
     CDS             complement(18768..19403)
                     /gene="mlaC"
                     /locus_tag="KLK57_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HBE0891045.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(19422..19973)
                     /gene="mlaD"
                     /locus_tag="KLK57_000580"
     CDS             complement(19422..19973)
                     /gene="mlaD"
                     /locus_tag="KLK57_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HBE0891046.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(19978..20760)
                     /gene="mlaE"
                     /locus_tag="KLK57_000581"
     CDS             complement(19978..20760)
                     /gene="mlaE"
                     /locus_tag="KLK57_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HBE0891047.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDTIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(20768..21577)
                     /gene="mlaF"
                     /locus_tag="KLK57_000582"
     CDS             complement(20768..21577)
                     /gene="mlaF"
                     /locus_tag="KLK57_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HBE0891048.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            21787..22764
                     /locus_tag="KLK57_000583"
     CDS             21787..22764
                     /locus_tag="KLK57_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HBE0891049.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIEPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            22778..23764
                     /gene="kdsD"
                     /locus_tag="KLK57_000584"
     CDS             22778..23764
                     /gene="kdsD"
                     /locus_tag="KLK57_000584"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HBE0891050.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            23785..24351
                     /gene="kdsC"
                     /locus_tag="KLK57_000585"
     CDS             23785..24351
                     /gene="kdsC"
                     /locus_tag="KLK57_000585"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HBE0891051.1"
                     /translation="MSKAGASLATCYGPVSADVIAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            24348..24923
                     /gene="lptC"
                     /locus_tag="KLK57_000586"
     CDS             24348..24923
                     /gene="lptC"
                     /locus_tag="KLK57_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HBE0891052.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGIDMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPNSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            24892..25449
                     /gene="lptA"
                     /locus_tag="KLK57_000587"
     CDS             24892..25449
                     /gene="lptA"
                     /locus_tag="KLK57_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HBE0891053.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            25456..26181
                     /gene="lptB"
                     /locus_tag="KLK57_000588"
     CDS             25456..26181
                     /gene="lptB"
                     /locus_tag="KLK57_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HBE0891054.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            26229..27662
                     /gene="rpoN"
                     /locus_tag="KLK57_000589"
     CDS             26229..27662
                     /gene="rpoN"
                     /locus_tag="KLK57_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HBE0891055.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDIGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHINTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            27685..27972
                     /gene="hpf"
                     /locus_tag="KLK57_000590"
     CDS             27685..27972
                     /gene="hpf"
                     /locus_tag="KLK57_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HBE0891056.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            28090..28581
                     /gene="ptsN"
                     /locus_tag="KLK57_000591"
     CDS             28090..28581
                     /gene="ptsN"
                     /locus_tag="KLK57_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HBE0891057.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVSADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            28627..29481
                     /gene="rapZ"
                     /locus_tag="KLK57_000592"
     CDS             28627..29481
                     /gene="rapZ"
                     /locus_tag="KLK57_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HBE0891058.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            29478..29750
                     /gene="npr"
                     /locus_tag="KLK57_000593"
     CDS             29478..29750
                     /gene="npr"
                     /locus_tag="KLK57_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HBE0891059.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            29964..30596
                     /gene="yrbL"
                     /locus_tag="KLK57_000594"
     CDS             29964..30596
                     /gene="yrbL"
                     /locus_tag="KLK57_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HBE0891060.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKLSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(30593..31321)
                     /gene="mtgA"
                     /locus_tag="KLK57_000595"
     CDS             complement(30593..31321)
                     /gene="mtgA"
                     /locus_tag="KLK57_000595"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HBE0891061.1"
                     /translation="MSKSRLTVFSFVRRFLLLLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFSEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(31318..31971)
                     /gene="elbB"
                     /locus_tag="KLK57_000596"
     CDS             complement(31318..31971)
                     /gene="elbB"
                     /locus_tag="KLK57_000596"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HBE0891062.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(32201..34537)
                     /gene="arcB"
                     /locus_tag="KLK57_000597"
     CDS             complement(32201..34537)
                     /gene="arcB"
                     /locus_tag="KLK57_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HBE0891063.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPVDVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(34633..35562)
                     /locus_tag="KLK57_000598"
     CDS             complement(34633..35562)
                     /locus_tag="KLK57_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HBE0891064.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLINRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTVHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGLAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            36237..40697
                     /gene="gltB"
                     /locus_tag="KLK57_000599"
     CDS             36237..40697
                     /gene="gltB"
                     /locus_tag="KLK57_000599"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HBE0891065.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGHILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            40710..42128
                     /gene="gltD"
                     /locus_tag="KLK57_000600"
     CDS             40710..42128
                     /gene="gltD"
                     /locus_tag="KLK57_000600"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HBE0891066.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            42312..42791
                     /locus_tag="KLK57_000601"
     CDS             42312..42791
                     /locus_tag="KLK57_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HBE0891067.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
     gene            42808..43113
                     /locus_tag="KLK57_000602"
     CDS             42808..43113
                     /locus_tag="KLK57_000602"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0891068.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(43189..43653)
                     /locus_tag="KLK57_000603"
     CDS             complement(43189..43653)
                     /locus_tag="KLK57_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HBE0891069.1"
                     /translation="MMMGEVRSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIENEQAIILKPGMFAVFMLGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(43650..44525)
                     /gene="nanK"
                     /locus_tag="KLK57_000604"
     CDS             complement(43650..44525)
                     /gene="nanK"
                     /locus_tag="KLK57_000604"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HBE0891070.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELMGADARTIFTRAGQGDEQAQQLIHRSA
                     HVLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAVFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(44522..45211)
                     /locus_tag="KLK57_000605"
     CDS             complement(44522..45211)
                     /locus_tag="KLK57_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HBE0891071.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPIPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(45259..46749)
                     /gene="nanT"
                     /locus_tag="KLK57_000606"
     CDS             complement(45259..46749)
                     /gene="nanT"
                     /locus_tag="KLK57_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HBE0891072.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(46859..47752)
                     /gene="nanA"
                     /locus_tag="KLK57_000607"
     CDS             complement(46859..47752)
                     /gene="nanA"
                     /locus_tag="KLK57_000607"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HBE0891073.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(47874..48665)
                     /gene="nanR"
                     /locus_tag="KLK57_000608"
     CDS             complement(47874..48665)
                     /gene="nanR"
                     /locus_tag="KLK57_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HBE0891074.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGEFSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            49045..50367
                     /gene="dcuC"
                     /locus_tag="KLK57_000609"
     CDS             49045..50367
                     /gene="dcuC"
                     /locus_tag="KLK57_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBE0891075.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGVFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERIGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAEQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMKSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVKRTAIPMAVGFVVNMIATITLFY"
     gene            complement(50410..50907)
                     /gene="sspB"
                     /locus_tag="KLK57_000610"
     CDS             complement(50410..50907)
                     /gene="sspB"
                     /locus_tag="KLK57_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HBE0891076.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(50913..51551)
                     /gene="sspA"
                     /locus_tag="KLK57_000611"
     CDS             complement(50913..51551)
                     /gene="sspA"
                     /locus_tag="KLK57_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HBE0891077.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIELSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(51946..52338)
                     /gene="rpsI"
                     /locus_tag="KLK57_000612"
     CDS             complement(51946..52338)
                     /gene="rpsI"
                     /locus_tag="KLK57_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HBE0891078.1"
                     /translation="MAENQYYGTGRRKNSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(52354..52782)
                     /gene="rplM"
                     /locus_tag="KLK57_000613"
     CDS             complement(52354..52782)
                     /gene="rplM"
                     /locus_tag="KLK57_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HBE0891079.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQVSFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(53001..54128)
                     /gene="zapE"
                     /locus_tag="KLK57_000614"
     CDS             complement(53001..54128)
                     /gene="zapE"
                     /locus_tag="KLK57_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HBE0891080.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFARGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLHDETRAQMDKLWLALAGGKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            54322..54720
                     /locus_tag="KLK57_000615"
     CDS             54322..54720
                     /locus_tag="KLK57_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HBE0891081.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            54874..56241
                     /gene="degQ"
                     /locus_tag="KLK57_000616"
     CDS             54874..56241
                     /gene="degQ"
                     /locus_tag="KLK57_000616"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HBE0891082.1"
                     /translation="MKKQPQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPEQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMIAPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKSAIIALQIVRGNESI
                     YLLMR"
     gene            56331..57398
                     /gene="degS"
                     /locus_tag="KLK57_000617"
     CDS             56331..57398
                     /gene="degS"
                     /locus_tag="KLK57_000617"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HBE0891083.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
BASE COUNT        14266 a        15301 c        14255 g        13610 t
ORIGIN      
        1 aagcgcatta ttttacaaaa aacacactaa actcttcctg tctccgataa aagatgatta
       61 aatgaaaact catttatttt gcataaaaat tcagtgagag cggaaatcca ggctcatcat
      121 cagttaatta agcagggtgt tattttatga cgacgattct caagcatctc ccggtaggtc
      181 aacgtattgg tatcgctttt tccggcggtc tggacaccag tgccgcactg ctgtggatgc
      241 gacaaaaggg agcggttcct tatgcatata ctgcaaacct gggccagcca gacgaagagg
      301 attatgatgc gatccctcgt cgtgccatgg aatacggcgc ggagaacgca cgtctgatcg
      361 actgccgcaa acaactggtg gccgaaggta ttgccgctat tcagtgtggc gcatttcata
      421 acaccaccgg cggcctgacc tatttcaaca cgacgccgct gggccgcgcc gtgactggta
      481 ccatgctggt tgctgcgatg aaagaagatg gcgtgaatat ctggggtgac ggtagcacct
      541 ataaaggaaa cgatatcgaa cgtttctatc gttatggtct gctgaccaat gctgaactgc
      601 agatttacaa accgtggctt gatactgact ttattgatga actgggcggc cgtcatgaga
      661 tgtctgaatt tatgattgcc tgcggtttcg actacaaaat gtctgtcgaa aaagcctact
      721 ccacagactc caacatgctt ggtgcaacgc acgaagcgaa ggatctggaa tacctcaact
      781 ccagcgtcaa aatcgtcaac ccaattatgg gcgtgaaatt ctgggatgag agcgtgaaga
      841 tcccggcaga agaagtcaca gtacgctttg agcaaggtca tccggtagcg ctgaacggta
      901 aaacctttag cgacgacgta gaaatgatgc tggaagctaa ccgcatcggc ggtcgtcacg
      961 gcctgggcat gagcgaccag attgaaaacc gtatcatcga agcgaaaagc cgtggtattt
     1021 acgaagctcc ggggatggca ctgctacata tcgcttatga acgtctgttg accggtattc
     1081 acaacgaaga caccattgag cagtatcatg cacatggtcg tcagttgggc cgtctgctgt
     1141 accaggggcg ttggtttgat tcccaggcgc tgatgctgcg tgactctctg caacgctggg
     1201 ttgccagcca gatcactggt gaagttaccc tggagctgcg ccgtgggaac gattattcaa
     1261 tcctgaatac cgtctcagag aacctgacct acaagccaga gcgtctgacg atggaaaaag
     1321 gcgactcggt gttctcgcca gatgatcgta ttggtcaatt gaccatgcgt aacctggata
     1381 tcactgatac ccgagagaaa ctttttggtt atgccaaaac tggcctgctc tcctcctctg
     1441 ccacttcagg cgtgccgcag gtggagaatc tggaaaacaa aggccagtaa ttcgacatca
     1501 accctgccct tcaggggtgg ggttaccttg cttatcctct gtaaactcct ttctgagcat
     1561 cacataatcg aaaacatcat ggtttgcatc cacaacccgt gagtctcctt tatagtgtgc
     1621 aatcacttcc tccagcgttt tcggttgttc cggctttgtt acccccatcc acgcattaat
     1681 aaggtaatta ttgtaagctg tcgaaaagat gttgttttcc gttgtacgat ccacgccatc
     1741 cccaagaacg gggctatacc agataaacat cgggacatga tatgcctgct ggctagcctc
     1801 cctgcctcca tgaaaataga cgttcttctt cgtagggtca cgttccaggc cgtggtcggc
     1861 aaaatacatg accgaggcgc ggcgatcttt taataattca aaaacctgac ctagcaaact
     1921 atctgtgtaa tgaatggagt tgtcatagca ggcatcctga tcgtcctgcg gttgaaacac
     1981 ggcgctggat tgcggatagg cgctacaagc cggttcatgg cttccattta aatgaagaac
     2041 aatcagtttc ttttgctgcg tattttgctg taatgcttgc gataaatgcg gcaacaacaa
     2101 ttcatcaaat cctctgacat aaactgtttc catggcgcgc atggcgatac tggtaactgc
     2161 tgtaccattc tgccgaaaag cggattgcga gcttagccag aaagtctgaa atcctgcttg
     2221 attagccata ttaataatgt tgtcggggta attatgaatg tcatgactca aaacagaatc
     2281 agcagttaaa gaaaggggaa ccgacagcgc agtgtaaggt gcgccgctta ttgattgatt
     2341 aaacagtttg atctgttttc tttgtgcttc aacttgcggt gtcgtagagc gtgtatatcc
     2401 atacaaagac atattgtcga cacgtacaga ctcccccaca atcaacacgt aggtatcaat
     2461 acctgtatcc ctgactgata attgaaaata cggcaccgtg tttgcaattg agagtaatct
     2521 ttgatgctct ttcgctgcta aagcaaaata attgagattg aaaaacggcg tataggtagc
     2581 aaatcgcgac gctagtatat atggactgaa tgctttttta ttttttgcat ctttataagc
     2641 aaattgacaa gcagaaaata aactgcccga aatgacaatc agcaataata ttcctgtcac
     2701 tttttttgtc ggcaaggaaa catcatattt tattattact gccaaaaaaa gaagggataa
     2761 aaaagcaaaa gcacatagat aagggatata catccccagc attttgacaa cttcatccgg
     2821 atcactctgc agcacactaa tcgcaaatcc atcattgaaa gttgtaccaa aggtacacca
     2881 tgaatagagg ctaatactca tgtctgccgc agtcagtaac acaaatggtg cggcaacaag
     2941 caggcgaagc ggaagggatg atatacgttt tacaaccagg atggtcagga cgaagaacag
     3001 caatcgctga accatatgcg cgccggaaga ggcgactaag ttcgtaatac caaaaacaat
     3061 aacacaaaga tacaacaacc aatgagattt aaaatcttct agcaaatttg ttgaatactg
     3121 tcatccagaa atccaagttc acaaccaata taaacaatta tcaacaatgt tcaattttat
     3181 atcagacatg tttttcaact ctgatccata tcagaccgaa acactgttag ttttttcttt
     3241 ttaaatgatg cggccattgc gttctcagta attaaataac attatcaatg cgtctctaaa
     3301 cattacgcag aacgtagcca tcagtagtta tactgacagg agcaggatgg tttatcgaga
     3361 agtaaacata tccgtattcg taccggaact ggagttgaca caataaagtg ccaattatgt
     3421 cagcagaagg gaaaagttta taactaaggc gtaccggcac catcgtttca aggtaccagc
     3481 tacgagtaaa gcaactggac gagatacaga tacctgacaa ccattcctca gaccaggacc
     3541 aaaacgaaaa aagacgcttt tcagcgtctc ttttctggaa tttggtaccg aggacgggac
     3601 ttgaacccgt aagccctatt gggcactacc acctcaaggt agcgtgtcta ccaattccac
     3661 cacctcggca cggatactac tttttagttc gggatatcgc tggtcggctt agccggagca
     3721 gctggctgag tttgttcggt tttcgccggt gcactcagat tttcccattc gctaccttta
     3781 ttggttttgt tgctattgat gttacccagc accagactga tgataaagaa taacgttgcc
     3841 agcagcgccg tcatgcgggt catgaagtta ccagaaccac ttgaaccaaa cagcgtagcg
     3901 gaagcgcctg ctccgaagga ggctcccata tcagcgcctt taccttgctg cagcatgatc
     3961 agaccaacaa ggccaattgc cacaataagg aaaactacca aaagagcttc gtacataatc
     4021 aacctgttcc ttgcggagtt gccgcgtacc aatgcttcaa ccaataaagc gggagttttt
     4081 aatctttccc actgaagtgg gtgtgaatac taaccaaagc gaatgacctt cgcaagggca
     4141 attttatcgc attgtatcaa ctgcggaaaa aaacagcaaa accgatgtgt tcgctgagaa
     4201 aaaaggcgac aatcgccgcc tttttagcca gttatttacc gttttaaacg gcttttactg
     4261 catcggcgat acggtgtgca aattcagtta cctgcgcttc gtcttcacct tctaccatca
     4321 cgcgaattaa cggttcggtg ccggatttac gcaacagcac acgtccacgg ctacccagcg
     4381 ccgcttcaac ttcggcggtc acagctttaa ctgactcatg ctcaagcgga tcgccgctac
     4441 ctgcggtgta acgcacgtta accagaatct gcgggaacat tttcatgccg ctgcaaaggt
     4501 cgtgcaggct catatggtta cgtgccatcg ccgccagcac ctgcaagcca gcaacgatgc
     4561 cgtcaccggt agtggtttta tccagcagga tcacatgacc ggaattctct gcaccgatac
     4621 gccagccttt ctcctgcatt ttttccagta cgtagcggtc acccactttc gcgcgcgcaa
     4681 atggaattcc taactgtttc aacgccagct caagccccat gttactcatc aatgtaccca
     4741 cagcaccacc acgcagctgg ccctgacgaa gaccttcacg cgcgatgata tacatgatct
     4801 gatcaccatc gactttattg ccttcatggt caaccataat cacgcgatcg ccgtcgccgt
     4861 cgaaggcaat accgagatcc gctttttcag ccagcacacg agcctggagc gcgcgaacgt
     4921 cggtagcccc cacttcggca ttgatgttta caccgttcgg ctcacaaccg atagcgataa
     4981 cgttcgcccc cagttcacgc agcacgttcg gcgcgatgtg ataagtcgca ccgtttgcgc
     5041 aatccaccac aatcttcagt tcgctgaggc taagttcgtt cgggaacgtg gctttgcaaa
     5101 actcgatata gcgacccgca gcatcaacga tacggctggc tttacccagt tctgccgaat
     5161 caacacagct gatctctttt tccatttccg cttcgatggc ctcttctacc gcatccggta
     5221 gtttggtgcc gtcgatagag aaaaatttaa tgccattatc gtagaacggg ttatgcgatg
     5281 cagatatcac aattccggcc tctgcgcgga aggtacgcgt cagataagcc acggccggtg
     5341 ttggcatcgg gccagtgaag agtgcggaaa ggcccgctgc cgccagaccc gcttccagtg
     5401 ctgactccag catatagcca gaaatacgcg tgtctttacc aataatgatc ttacgggagc
     5461 cgtggcgcgc cagcacttta cccgcggccc aacccagctt aagcacaaaa tcaggtgtga
     5521 tcggcgcatc ccctacacga ccacgaatcc catcggtacc gaaatattta cgattactca
     5581 tagcgtttgt tttcctttgc agacagagtg gcttccacca cccgcatcgc ttctacggtt
     5641 tctttgacgt catgaacacg aataatgtgc gcgccttgca ttgcggcaat gaccgcacag
     5701 gccagactac cgctcaggcg ctcggacggc cccacgttca gcagctgccc aatcatcgat
     5761 tttcgtgaca tacctaccaa cagcggcagg ttgaaatggt gaaattcagc caggcgcgcc
     5821 agtaatgaat agttatggga gagattttta ccgaaaccga atccggggtc gagcaacaat
     5881 ttctcttttg cgatacccgc ctgctcgcaa cgtgctattt gctcaataaa gtagcgattc
     5941 acttctgcaa agacatcgtc atacttcgga gcttcctgca tggtttttgg atttccctgc
     6001 atatgcatca gacaaaccgg taaaccggtt tctgcagccg cctccagagc gccaggttcg
     6061 gaaagggagc ggatatcgtt aataatgtga gcgccaactt tcgctgactc acggatgact
     6121 tctggtttgg atgtatctac cgagatccag acttcgaagc gttgagcaat tgcctcaacc
     6181 acaggaataa cacgttgcaa ctcttcttca acgctaactt ccgccgcccc tgggcgcgtg
     6241 gactcgccac caacgtcaat gatcgtcgcg ccagcgttga tcatcagatt cgcatgtttc
     6301 accgcatcta tcagcgagtt atgcgtgcca ccatccgaaa aggaatcagg cgtgacgttg
     6361 aggatcccca ttacgtgagg atggctaagg tccagtgaag tgccctgggc aaagagtttc
     6421 atggtgttat ccctggtatg aattgataag aaaaaacccc ggagcacgcc ccggggtttt
     6481 cggtacaaat acagtcatct gatgcgggaa cttacttgtc gcctaactgc tctgacatgg
     6541 tgttacccgg gttcggcgta cgcggttcat caaccggacg aggagccttt ggactaccat
     6601 tgtcgccaga attgttagaa gcgcctggtt cttcccagcc cgctggcgga cgtacatcgc
     6661 gacgtgccat caggtcatca atctgcggtg cgtcgatagt ctcatatttc atgagagcat
     6721 ctttcatcgc atgcagaata tccatattgt cggtcagaag ctgacgcgca cgattatagt
     6781 tacgctcaat cagtgctttc acttcctggt cgatgatacg tgcagtttca tcggacatat
     6841 gtttcgcttt cgctacgcta cggccgagga acacttcacc ttcttcttcc gcgtacagca
     6901 gtggacccaa tttctcagag aagccccact gagtcaccat gttacgcgcc aggttggtcg
     6961 caactttaat atcgttggac gcaccggtag aaacatgttc cggcccgtag atgatctctt
     7021 ctgccagacg accaccgtac agcgtagaaa tctggctttc cagtttctga cggctggcgc
     7081 tgattgcgtc gccctcaggc aagaagaaag tcacacccag cgcacgaccg cgtgggataa
     7141 tcgtcacttt gtgcaccgga tcgtgttccg gcaccaggcg accgataatc gcatgacccg
     7201 cttcgtggta agccgtcgat tctttctgcg cttccgtcat caccatggag cgacgttccg
     7261 cacccatcat gattttatct ttcgctttct cgaactcaac catcgacaca acgcgtttgt
     7321 tgccacgagc agcgaacagt gccgcttcgt ttaccaggtt cgccaggtca gcaccggaga
     7381 aaccaggagt accacgggca atgattgccg cgtcgatatc gggtgccaat ggtacgcgac
     7441 gcatgtgaac tttcaggatc tgctcacgac cgcgaacatc tggcaagcca accacaacct
     7501 gacggtcgaa acggccagga cgcagcaggg ccgggtcaag aacgtccgga cggttagtcg
     7561 cggcgataac gatgatacct tcgttacctt cgaagccatc catctcaacc agcatctggt
     7621 tcagagtctg ttcacgttca tcgtgaccac cgcccagacc agcgccacgc tggcggccta
     7681 cggcgtcgat ttcatcgata aagatgatgc acggtgccgc tttcttcgcc tgttcgaaca
     7741 tgtcacgaac acgggatgca cccacaccga cgaacatttc tacgaagtca gaaccggaga
     7801 tagtaaagaa cggaactttc gcttcgcctg caatcgcttt cgccagcagc gttttaccgg
     7861 tacccggagg accgaccatc aggacgcctt tcgggatctt accgccgagt ttctggaagc
     7921 ggctcggctc gcgcagatat tcaaccagtt cagcaacttc ttcttttgct tcgtcgcagc
     7981 ccgcaacgtc agcaaaggtc gttttgatct gatcttccgt cagcatgcgc gctttgctct
     8041 taccaaacga catggcacct ttgccaccgc cgccctgcat ttgacgcatg aagaagatcc
     8101 acacaccaat cagcaacagc atcgggaacc aggagatgaa gatagaagcc agcaggcttg
     8161 gttcttcagg cggttcaccg acaaccttga cgttcttggt caacaggtta tccagtaatt
     8221 tcggatcctg aaccggaatg taagtggtat aacggttact atctttcttg gtaacgttga
     8281 tttcacgtcc gttgatacgc gcttcacgaa cctggtcgtt attgacctct tgtaggaagg
     8341 tagagtaatc caccttacgg ccattagact cgctgggccc aaagctctgg aatactgaca
     8401 tcagcacaac ggcaatgacc agccagagta ttaggttttt cgccatgtca ctcaagggat
     8461 taacctctta ttacaactgt gttaaaaaca gcgtcaggat actctatatc cagcatcttt
     8521 caaactttcg tctgaaatct cccggttagg gtttacgccc ggtcgctaca atatacactt
     8581 cccgcgaacg tgcacgagaa gagtccggct tacgaacttt gaccttcgta aacagggagc
     8641 gaatttccct tagatactca tcgaaacctt cgccctggaa caccttcact acaaaactgc
     8701 cacctggcgc taatacatca cgacacattt ctagcgccag ttccaccaga tacatggcac
     8761 gggggatatc caccgccggt gttccgctca tgtttggtgc catatcggac atgacaacct
     8821 ggactttgct gtcgccaacg cgctccagca gtgctttcat caccagttca tcacgaaaat
     8881 cgccctgaag aaagtctaca ccaacgatag gatccatagg taaaagatcg caagcgatga
     8941 tgcggccttt gccgccaatt tgggtgacca catattgtga ccaaccaccc ggagcagcac
     9001 caaggtcgac aaccgtcatt cccggtttaa agagtttgtc actttgctgt atttcatcaa
     9061 gtttaaacca ggcacgggaa cgtaacccct ttttctgtgc ctgttgaaca tatttatcgc
     9121 taaagtgttc ctgaagccag cggctggagc tggcagaacg cttcttacct gtcatttaac
     9181 tttcccatgg ggataactca tcgtaaccaa ttgcgtaaat ttttacgcgc ctatttggtg
     9241 atatatggga gatggcggta gaatgaccca ttttcaatcc caacgtaagc aaaaaatacg
     9301 atgaatctga gtactaaaca aaaacagcac ctgaaaggtc tggcacatcc gctcaagcca
     9361 gttgttctgc ttggcagtaa tggtttgacc gaaggggtgc tggccgagat tgaacaagcg
     9421 ttagagcacc atgaactcat caaggtgaaa atcgccaccg aagatcgcga aacaaaaacc
     9481 ttgatcgtgg aagctatcgt gcgcgaaacc ggcgcctgta atgtacaggt catcggtaaa
     9541 acgctggtgc tttatcgccc aactaaagaa cgtaaaatct cgctgccacg ctaagattat
     9601 cctaaagtta cacacatttg ctgtgtaaaa cgaggggttt tccgcaggca ggagagcaaa
     9661 atgccacgct ctgttcgttg ataaaaggcc gcatagcggc ctttttcctt tctttacaat
     9721 acatcaacat cttgagtatt gggtaattct tacaggtatt ccaccttaat tacttcaaat
     9781 tctacttcgc cgcccggcgt tttgatgacc acaacatcat cttcttcttt gccgatcagg
     9841 ccacgagcaa taggcgagtt tacagaaatc aggttttgtt taaagtcagc ttcgtcatcg
     9901 ccaacgatgc gataagtctg ttcttcgtca gaatccagat tcagcaccgt tacggtagca
     9961 ccaaaaataa cgcgcccatt gttaggcatt ttggtgacat caatcacctg cgcgtttgac
    10021 agcttggctt cgatgtcttt gatacggcct tcgcagaagc cctgctgttc acgagctgcg
    10081 tggtattcgg cgttttcttt caggtcgcca tgctcacgcg cttccgcgat agcagcaatg
    10141 atttcaggac ggcgcacaga tttcagaaaa tccagctctt cgcgtaattt ttcagcgccg
    10201 cgtaaggtca tcggaatagc ttgcatttgt tatacctctt gaatattcct gatggggcaa
    10261 gtcttcaccc accccggctg ttcggcctgc ccggcataac tcctcaccgg gaccagaagc
    10321 aaaaaaatac cgacccaggt acaagtccca ggtcagctac aattcacatt ttgatagtca
    10381 ttttaccctg aagttcccga agggtcatcg tttactttat agggcgttgc gccgtagtat
    10441 gacggctcga ttccaggttg ttagcgcgag attatgcgat tttccagatt tatcatcgga
    10501 ttgaccagct gtatagcgtt cagtgttcag gccgcaaatg ttgatgagta cattactcaa
    10561 ctccccgctg gtgccaacct tgccctgatg gtgcaaaaag tcggcgcgtc ggcccccgct
    10621 attgattacc acagtcagca gatggcgctg cctgccagta cccagaaagt gattactgcg
    10681 ctggcggcgt tgattcaact cggccccgat tttcgtttta ccacgacgct tgaaaccaaa
    10741 ggcaatgtgg aaaacggcgt acttaagggt gacttagtgg cgcgatttgg tgccgatccg
    10801 acgttaaaac gtcaggatat tcgcaatatg gtcgcgactt tgaaaaaatc tggcgtcaac
    10861 caaatcgatg gcaatgtgtt gatagatacc tccattttcg ccagccacga taaagccccc
    10921 ggctggccat ggaatgacat gacacaatgc tttagcgctc cgcctgccgc cgccatagtt
    10981 gaccgcaact gtttctccgt ctcgctctac agtgcccaaa aacccggtga tatggccttt
    11041 atacgcgtgg catcttatta ccccgttacg atgttcagcc aggtacgcac cctcccccgt
    11101 ggttctgccg aagcgcaata ctgcgaactg gatgtcgtac caggcgacct gaaccgcttt
    11161 acgctgacgg gatgcctgcc acaacgttct gagccgctcc cgttggcttt tgccgtgcag
    11221 gatggagcca gttatgccgg tgcaattctg aaagatgagt taaaacaggc gggtatcacc
    11281 tggagcggaa cactgctgcg ccagactcag gttaacgaac ctggaacggt agttgccagt
    11341 aaacagtcgg ccccgctgca cgatctgctt aagattatgc tgaaaaagtc ggataacatg
    11401 atcgccgata cggttttccg catgataggc catgcgcgct tcaatgtacc tggtacatgg
    11461 cgggccgggt cggacgctgt gcgtcagatc ctgcgccagc aagccggtgt cgatattgga
    11521 aacaccatta ttgccgatgg ttcagggctt tcgcggcata acctgattgc ccccgccacc
    11581 atgatgcagg tgctgcaata cattgcccaa cacgacaatg aacttaactt tatctccatg
    11641 ctgccgctgg cgggctatga cggctctttg cagtaccgtg caggtctgca tcaggcgggc
    11701 gtggatggaa aagtctcagc gaaaaccggt tcgttgcagg gggtatataa cctggcggga
    11761 ttcattacca cagcgagcgg gcaacgaatg gcgtttgtgc aatatctttc tggctatgca
    11821 gtagaacctg cggatcagcg taatcgccgt attccgttag tgcgttttga aagccgtttg
    11881 tataaagata tttatcagaa caattagtca aaaagaaacc ccggcacatg gccggggctt
    11941 cagattattg acaaagtgcg ctttgtttat gccggatgcg gcgtaaacgc cttatccggc
    12001 ctacaaaatc gtgcaaattc aacatattgc aattctcttg taggcctgat aagcgtagcg
    12061 catcaggctg atttggcgtt tatcatcagt gattaacgct tgtaaatgaa ctcaacgcct
    12121 tcttcgtcgt cttcgtccca gtcgtcatcc cagtcttcat catcctcttc agcaatctct
    12181 tcaagctgct ggcgatgata atcgtcccac atgaattcga ctttctctgg ctgtttcgct
    12241 tcttcagcct gcacgaccgg gttttcaagg ataaaggtca tcacatccca gcagagatct
    12301 ttcacgccca gtccgctcgc cgcagagatc agataatatt tatcttccca gcccagcgct
    12361 tcagcaatcg ctttcgcttt ctcttcagct tctgccttat ccagcagatc gatcttattg
    12421 aacactaacc aacgcggttt cgccgccaga tcctggctgt atttttccag ctcgctgata
    12481 ataatacgcg cgttttcaac cggatcggtg ccgtcaatcg gatcgatatc gatgaggtgc
    12541 aacaggacgc ggcaacgttc caggtgcttc aggaagcgga tacccagacc tgcgccttcc
    12601 gcagcgcctt caatcagtcc tggaatatcg gcaacaacga agctcttttc gttgtccatt
    12661 cgtaccacac ccagacttgg caccagagtg gtaaacggat aatccgccac tttcggttta
    12721 gccgccgata ccgcgcgaat aaaggtcgat ttacccgcgt ttggcatccc caacataccg
    12781 acgtcagcca gcagcatcag ctccagcagc agctcacgct tatcacccgg cgtaccgttg
    12841 gttttctgcc gtggtgtacg gttaacggac gatttgaaac gggtattgcc cagaccgtgc
    12901 cagccgccct tagcaaccag cagacgctga ccgtgtttag tcatatcgcc catggtttca
    12961 ccagtaccct ggtcgattac acgcgtacct accggcactt taatcgtcac gtctttaccg
    13021 cgcttaccgg tacaatcgcg gcttgcgccg ttctgaccgc gctctgcacg gaaagatttt
    13081 tcaaaacgat aatcgataag cgtgttcagg ttttcgtcgg cttccatcca tacgtcacca
    13141 ccatcaccac cgtcgccgcc atccgggccg cctttcggaa tatacttttc gcggcggaag
    13201 ctcacgcaac cattaccgcc atcacctgca acgaccagaa tcgatgcttc atcaacaaac
    13261 ttcattttat tctccataaa tcattcgcct gcgcggggtt gcgataccac cgttgtatgc
    13321 ttacgtaatc cgccccaaat acgatgacca atggcggaat acatcgcgcc cgcaaccacg
    13381 acaaacgcac cgagataacc taaaaggttt aacatcggtc tggcgaagaa atcgggccag
    13441 gccagtgata aaagatctga aaaaaacagc gtaaacagtg gggtgagcgt gatgatcgcg
    13501 ctcacctgcg ctgcctgcca gcgagccatc gcttccgcca gggcgccata tcctaccaat
    13561 gtattcagtc cgcaaaaaat taaacatgcg agctgccagt ggctaagctg cgctatcact
    13621 ccaggctttg ccagagggaa gagcgcaatt gtacataaag tgtacagtaa aaacaggatc
    13681 tgcggtgagg ccagccgacg caataaaacc ttttgcgcca cgccataact cacccaaacc
    13741 gtcgccgcac cgaccccaaa gataactccc caggtgtaat cggtgagctt tgtaaatatc
    13801 tcgaccagac tggtgttaaa aaacatcacc aggccgctca ggagcatcaa tgcccctaca
    13861 acctgagtgc tgcgcatttt ctctttcagg ataaatacgc tggcaaccat catgccaact
    13921 ggcgagagtt gcccaatcac ctgcgaagcg gtcggactca ggtattgcaa ggatgagctg
    13981 aacaggatga agttcccaaa cagcccggcg gtcgccactg ccaacaaaat caaccagcgt
    14041 ggcttacgaa acacgcgtaa tggcggcaac ctcttcttca ccgcaagaat ggcaccaagg
    14101 ccaatactcg ccatcaagaa acggtaaaac acgattgtcg gaggttccat cacctccagc
    14161 acctgcttca ttgcgattgg caacgccccc cagcaaattg ctgtggtgag cgccaaaaga
    14221 atgccaatgc ctgcctgctg cttcataccg tattccctgc aaagcgcatt tgcggattaa
    14281 cgtcgcacca tgcgcatttt taccggttat cgaatgtaaa aagccccgca acgtgttgcg
    14341 gggctttcat ccgttaccgg gacgcgaaaa acttattcag cttcgatgct gataaattta
    14401 cggtttttcg ggcctttaac ttcgaatttc actttaccgt ctgctttagc aaacagagtg
    14461 tggtcacgac cgcaacctac gttagcgcca gcgtggaatt tggtaccacg ttgacgaacg
    14521 atgatgctac ccgccagaac ggattcgcca ccgaaacgct taacgcccag gcgtttagct
    14581 tctgaatcgc gaccgttacg tgtggagccg ccagcctttt tatgtgccat ttgaaatctc
    14641 tcctcaggtc ttaggcgctg atgccagtaa ttttcacatc agtgaaccac tgacgatgac
    14701 cctgctgctt acgatagtgt ttacgacgac gaaacttaac gattttaact ttctcgccac
    14761 gaccgtgagc aacaacttca gctttgatta cgccgccatc aacgaaagga acgccgattt
    14821 tgacttcttc accgtttgcg atcatcagca cttcagcgaa ctcaacagtt tcgccagttg
    14881 cgatgtccag cttttccagg cgaacggtct gaccttcgct tactcggtgt tgtttaccac
    14941 cactttggaa aaccgcgtac atataaaact ccgcttccgc gcacaccttt tcaatgattc
    15001 agagtgcgct ataaatattc acaatagggc gcgaatatta cgcaaaacgc acgcctttga
    15061 caagtgctac agtcaataca cgaagaaaaa aaacacaact tgtacggtaa cgtttatctg
    15121 tgccattttt tcagtacaat caccctatat tcctaaccat aaaccctaag ttgcctttgt
    15181 tcacagtaag gtaatcgggg cgaaaagccc ggcttttgcg atgaatttag aaaaaatcaa
    15241 tgagttaacc gcgcaagata tggcgggtgt taatgcggca atccttgagc agcttaattc
    15301 cgacgtccaa ctgatcaatc agttaggcta ttacatcgtc agcggcggcg gtaaacgtat
    15361 tcgtccgatg attgctgtac tggctgcacg agctgttggc tatgagggaa atgcgcatgt
    15421 caccattgct gccctgatcg agtttatcca cacggcgact ctgctacacg acgacgttgt
    15481 ggatgaatca gatatgcgca ggggtaaagc taccgccaac gccgcatttg gcaatgccgc
    15541 cagcgtgctg gtaggcgatt ttatttatac ccgcgcgttc cagatgatga ccagcctcgg
    15601 ttcactcaaa gtgctggaag tcatgtcaga agctgtaaac gtcatcgcag aaggtgaagt
    15661 tctgcaactg atgaacgtta acgatccgga catcactgaa gaaaactaca tgcgcgttat
    15721 ctatagcaaa accgcgcgtc tgtttgaggc tgccgcgcag tgttccggga ttctggctgg
    15781 ctgtacgccg gaggaggaga aaggcctgca ggattatggg cgctatctcg gcactgcttt
    15841 ccagttgatc gacgatttac tcgattacaa tgccgatggc gaacagttag gtaaaaatgt
    15901 cggcgacgat ctgaacgaag gtaaaccgac gctgccgctg ctgcatgcga tgcatcatgg
    15961 cacaccagaa caggcacaga tgatccgtac cgccatcgaa cagggtaacg gtcgccatct
    16021 tctggaaccg gttctggaag caatgaacgc ttgtggatct cttgaatgga cgcgtcagcg
    16081 tgccgaggaa gaagcagaca aagccatcgc agcattacag gtgctcccgg acaccccttg
    16141 gcgagaagca ctcatcggcc tcgcgcacat cgctgttcaa cgcgatcgtt aatcccctcc
    16201 cttcatcccg cgcagtgcgc gggatgattc cagtaaattc cataaaaaca ctaattcagc
    16261 gctataacac ctgaaaaaaa agggcgatga atctttatat attctgaata ttcacactct
    16321 ttacaggaac tttttagagc aataggccat caggagtata gtgatgcccg acagaagaag
    16381 tgttctgaat gaaagcgact aacttaagga gtgaggaaag atggaaagta atttcattga
    16441 ctggcatccc gctgacatca ttgcgggttt gcgcaagaag ggaacgtcaa tggcggcgga
    16501 gtctcgcaga aatggtttga gttcctcaac gctggcaaac gcattatcgc gcccatggcc
    16561 gaaaggagag atgattattg cgaaagccct gggaactgac ccctgggtta tctggccatc
    16621 acgctaccat gatccgcaga cccatgagtt tatcgacaga acgcagttga tgcgtagcta
    16681 cactaaaccg aaaaaatgag tggtctggcg gtagccccgc gaacggggct gccagctctc
    16741 agacgattac tcgcctttca cacgctcaat atttgcacct aaagcgcgca gtttgtcttc
    16801 aatgcgttcg tagccacgat cgatgtgata aatacgatca accaccgtcg tcccttccgc
    16861 aatacagcca gccagcacca ggcttgctga tgcacgcaga tcggttgcca taacctgtgc
    16921 gccagaaagt ttttcaacac cgtgacaaat aacggtattg ctttcgattt cggcatgcgc
    16981 gcccatacgg ctaagctccg gcacatgcat aaagcggttt tcaaagaccg tttcggtgat
    17041 gaatccggtc ccttctgcca ccaggttcaa cagcgtgaac tgggcctgca tatcggtcgg
    17101 gaatgccgga tgcggcgcgg tacgtacgtt aacagccttc ggacgtttgc catgcatatc
    17161 caggctaatc cagtcttcgc cgacttcgat gtccgctcca gcgtcacgca gtttcgccag
    17221 cacggcgtcg agagtatctg gctgcgcgtt acggcagata attttgccgc gagaaatcgc
    17281 cgccgccacc aggaaagtac cggtttcgat acgatccggt agcacgcgat agacaccgcc
    17341 gcctaaacgt tccacacctt cgatgacgat acgatcggtg ccctgaccgc taattttcgc
    17401 acccagcgta atcaggaagt tcgcggtatc gacgatttcc ggttcacgcg ctgcattttc
    17461 aataatcgtg gtaccttccg ccagggttgc agcacacatg atggtcaccg ttgcgccaac
    17521 gctgacttta tccatcacga tatgtgcgcc tttcagacga ccatcgacgg aagctttaac
    17581 gtaaccttct tccagtttga tggtcgcgcc taattgttcg aggccagaaa tgtgtagatc
    17641 aaccggacgc gcaccgatcg tacaaccgcc aggcagtgaa acttgcccct gaccaaagcg
    17701 cgctaccagc ggccccagtg cccagataga agcacgcatg gttttaacca gatcgtaagg
    17761 tgcgcagaat acattaacgt cgcgggcatc aatatgcaca gaaccattac gttctacttt
    17821 cgcacccagc tggcttagca gcttcattga tgtatcgacg tctttcagtt ttgggacgtt
    17881 ctggatctct accggttctt ccgccagtag agcggcaaaa aggataggca gagcagcatt
    17941 tttagcgccg gaaattgtga cttcgccctg gagcttcgtt ggcccctgaa cacgaaattt
    18001 atccatttag tttgttctca gttaacaatt catatccgct accggcgaat cgcccatagc
    18061 tcaaaagccg ttcagtttgc gatcgcgcgc ccactccgca ggggtatacg ctttgatcga
    18121 cacagcatga atgcggttat ccgcaatata ttccatcagc gggccataga ccgtctgctg
    18181 ttttttaacc cgactcatgc cgtcaaacaa ctcacccacg gcaataacct gaaagtggct
    18241 gccatcgccg gaaacgtgga cttcctggag ggagagagcg ttcatcaaca cgctctgaat
    18301 ttcattattt tccatgggat cttcaatcat cagttaataa accagcgaaa catcttagag
    18361 caaagttgcg ctggcataaa taagcaaaaa gcctcgctga taaatcagac aaggctcgac
    18421 ttgcaggcag gtttgccgga caggcggtta acgccatatc cggcctgaaa aaatttaacg
    18481 aggcagaaca tcagcaggca aattatacaa tttcgcgagg gtatacactt tgtcgtttac
    18541 cccctgaagc gtcacgttgt tgccctgctt tttcgccaga tcgataagat ggagcagcag
    18601 tgccagtcca cccgtatcca cgcgggacac acggctaaga tcgatgcagg taatcccctt
    18661 caccgcttcc tcacgcattt cccaaagcgg tagcagaacg tcctgatcca gctctccgga
    18721 taacgccagc gtgtcacccg tctgcatcca gctcagtgac tcgctcatta ttttttctct
    18781 tccagagtga ttttctgttg agaaatcgat ttcagttgcg cagtcaggcc gtcgatacct
    18841 ttggtacgca gcagcgttcc ccactcgttt tgtttggtgg tgatcatact gacgccttca
    18901 gcaatcatgt cgtaagcctg ccagtttccc gtctgggagt ttttacgcca ctggaagtcc
    18961 agacgcaccg gcggacggcc attcgggtca ataatggtaa cgcgaatagg cacaatggtt
    19021 ttatcgccca gcggctgttc tggcgcaatc tgataggttt gaccgtgata catcgccagc
    19081 gcctgaccgt aagcctgctt caggtactca cggaaagcgg caaagtaggc ttcacgttga
    19141 gcaggggtcg cactcttgta atactggccc agcaccagcg caccggcgta tttcacctgt
    19201 acgtatggca gcagttcctg atcaacaatg gtacgcagat aatccgggtt ggcccgaatt
    19261 tgcggttgct cattcttcag gcgatcgaac gttttctgcg ccgcctcgtc catcagctta
    19321 taaggattgg tctggtctgc cgcggttgcc gcactcagag gtgcaatcac cagcaaagcg
    19381 accatcatta aacgtttaaa catgcgtcgg ttctcctgaa attatttcgt tgtacccaca
    19441 ggttcagtgg tttcattatt acctggcgca gcagctggcg catcgccact attcttattg
    19501 tcatcgcctt tactaccgta aaggaactga ccaatgagat cttccagcac catcgcagac
    19561 ttagtgtcct gaattgtatc gccatccttc aggatagcag tccccagttc cgggtcttca
    19621 aaaccgacgt ttaatgccag atattgttcc cccagcaggc cggaagtacg aatgctcagc
    19681 gaactggtat ctggaatgtg gttataacgt tgttcaattt ccagcgttac gcgcggcaga
    19741 taggttttcg ggtccagcgt aatatccgcc acccgaccca caacaacgcc accaatactg
    19801 accggagagc gcgctttcag gccgccaatg ttatcgaacg tcgcataaag cgtgtaggtc
    19861 ggttcagtac gtatggacgt cacgttcgcc gccttcaggc aaacaaacag cgccgccagc
    19921 agtgctgcta ataaaaagat acccacccaa atttcatttt ttttcgtttg catgaactca
    19981 attcccaaac atcaatgcgg tcagcacaaa atccagcccc agaacagcca gagacgagtg
    20041 gacaacggtg cgagtggttg cccggctaat cccggcagac gtcgggatgg tgtcgtagcc
    20101 gttaaacaac gaaatccacg tcaccgtgat ggcgaacacc acgctcttaa tcagacagtt
    20161 gaccagatcc atacgccagt cgacggcatt ttgcattgcc gaccagaaga acccgctatc
    20221 aatgcctttc caactgacgc cgaccagaga tccgccccag atccccacgg cgacgaaaat
    20281 aaccgtcaac agtggtaatg aaataacccc agcccagaaa cggggagaaa taacccgacg
    20341 cagcggatcc accgccatca tctccatact ggagagttgc tctgtagcgc gcatcaggcc
    20401 gatttctgcg gttagcgccg aaccagcacg cccggcaaac aacaacgcgg caaccaccgg
    20461 ccccagttca cgcagtagcg ataacgccac cagcataccc agactggttt ccgcactata
    20521 agtggtcaga accagataac cttgcagccc caacaccatt ccgatgaaca cgccagaaac
    20581 cacaataatc agcatcgaca ggacgccgac attatagagc tggcgcacca gcagcggcgc
    20641 atgtttgcga aattccggtt tgccgaccag cgcattgaat aacattaacc cggcccgccc
    20701 gaacgttctc agggttttaa tccctttatg tccgagcgac gccagcgcat ttaacagcat
    20761 gagtggctta actccctggt aaaagatcag cgtgataatc gccggcagga tagcggaacg
    20821 gaacaggccc gtcagctatc ccgtccagaa actgacgtac gcgcggatca ggattcgcct
    20881 gcaacgcctg ggcactgcca tgagcgacaa tttttttgtc cgccaggatc caggcgtgat
    20941 ccgcaatact taacacttcc ggcacatcgt gagaaaccac cacacaagtc acgcccagcg
    21001 cgctgttcag ctcagaaatc agcttcacca gtacgcccat ggtgatggga tcttgcccaa
    21061 caaaaggttc atcaaacatg atgagatccg gctccagcgc aatcgcacgc gccagcgctg
    21121 cacgccgcgc catcccaccg gaaagttcag aaggcattag tttagccgct ccacgcagcc
    21181 ccacggcctc cagcttcatc atcaccgtac tatgcaacaa tggcgcgggt agttgggtat
    21241 gttcgcgcag tggataggcg acgttgtcaa atacgttcat atcagtgaac aacgccccgg
    21301 actgaaataa catgctcatc cgtttgcgca ctgtatacag gcgcgaacga gacatcgccg
    21361 gaatattctc accatcgaaa aggatctcac catgatctgg tgcgatttgc ccgccaatca
    21421 gacggagtag cgtcgtttta ccgatgcccg atggccccat gatcgccgtg atcttccctc
    21481 gcggcacggt cagggaaata ttatcgaaga tgcagcgatt gccacgcgta aaactgacat
    21541 cgcgcatatc gactaaattc gccacagact gctccataat tcacccttcg tcttgcgttg
    21601 attttctaag catggcgctc aatttaacct tgaacccaac atatttacag aatattaccc
    21661 gccgtggtta gcgaaagctg gcatttgttt tactttttag ccgcataaag tcaaaattaa
    21721 gaatccgtta cggctttctg aaaatcttca gcggaccgac gagtatacct gaagaaagga
    21781 cgttagatgc ttttagctac ggcactgtta attgttggtt tacttttggt cgtttacagt
    21841 gccgaccgcc tggtttttgc cgcgtctatt ctttgccgaa cctttggcat cccgccgctg
    21901 atcatcggca tgacggtggt cagtattggt acatcgttac cagaaatcat cgtctcgctt
    21961 gccgcgtctc tgcacgaaca acgcgattta gccgttggta cagccctcgg ctcaaacatt
    22021 atcaatattt tgctgatcct cggtctggct gcgctggttc gtccttttac cgtccattcc
    22081 gatgttctgc gtcgtgaatt acccttaatg ttgttggtca gcgtagtggc cggttccgta
    22141 ctctatgacg gacaacttag tcgcagcgat ggtatctttc tcctctttct ggctgtgcta
    22201 tggctgctgt tcattgttaa acttgcacgt caggctgaac gtcaggggac tgacagcctg
    22261 accagagagc agcttgcaga gctgccgcgt gacggcggat tgcccgtcgc gtttttatgg
    22321 ctcggcattg cgcttatcat catgccagtg gccacgcgga tggtggttga taacgccacg
    22381 gtgctggcga attactttgc catcagcgag ttgacgatgg gtctgacggc aattgctatc
    22441 ggaaccagcc tgccggaact ggcaaccgca atagcggggg ttcgcaaagg tgaaaacgac
    22501 attgctgtcg gaaatatcat tggcgcaaac atttttaata ttgtcatcgt gttgggttta
    22561 cccgcgctga taacgccagg agagattgag ccattggcgt acagtcgtga ctacagcgtg
    22621 atgttgctgg tgagcattat ttttgcgttg ctgtgctggc ggcgctcccc gcaaccgggc
    22681 cgtggtgtag gggtattatt aactggcgga tttatcgtat ggctggcgat gttgtactgg
    22741 ttatcgccaa tactcgttga ataactggaa acgcattatg tcgcacgtag agttacaacc
    22801 gggttttgac tttcagcaag caggtaaaga agtcctggcg attgaacgtg aatgcctggc
    22861 ggagcttgat caatacatca atcagaattt cacgcttgcc tgtgaaaaga tgttctggtg
    22921 taaagggaaa gttgtcgtca tggggatggg gaaatcgggg cacatcgggc gcaaaatggc
    22981 cgcaacgttt gccagcaccg gtacaccttc atttttcgtc catcctggtg aagccgcgca
    23041 tggtgattta ggcatggtca ccccacagga tgtggtgatt gctatctcta actcaggtga
    23101 atccagcgaa atcacggcct taattccagt gcttaagcgt cttcacgtac cgttaatctg
    23161 catcaccggt cgcccggaga gcagcatggc gcgcgccgca gatgtgcatc tgtgtgttaa
    23221 agtagcgaaa gaagcctgtc cgttagggct ggcaccgacc agcagcacca ccgccacgct
    23281 ggttatgggc gatgccctcg ctgtcgcgct gttaaaagca cgcggcttta ctgctgaaga
    23341 ttttgcgctc tcacacccag gcggcgcact gggtcgtaaa cttctgctgc gcgtaaacga
    23401 tattatgcat acgggcgatg agatcccgca tgttaagaaa acggccagtc tgcgtgacgc
    23461 gttgctggaa gttacccgca aaaatcttgg tatgactgtc atttgcgatg acaatatgat
    23521 gattgaaggc atctttaccg acggtgattt acgccgtgtc ttcgatatgg gcgtggatgt
    23581 tcgtcagtta agtattgccg atgtgatgac gccgggggga atacgtgtgc gccctggcat
    23641 tctggccgtt gaggcactga acttaatgca gtcccgccat atcacctccg tgatggttgc
    23701 cgatggcgac catttactcg gagtgttaca tatgcatgat ttactgcgtg caggcgtagt
    23761 gtaaagattc aaggataaac aacaatgagc aaagcaggtg cgtcgcttgc gacctgttac
    23821 ggccctgtca gcgccgacgt tatagcaaaa gcagagaaca ttcgtctgct gatcctcgat
    23881 gtcgatggcg tactgtcaga tggcctgatt tatatgggca ataatggcga agagctgaaa
    23941 gcgttcaatg ttcgtgacgg ttatggcatt cgttgtgcgc tcacctctga tattgaagtc
    24001 gctatcatta ccgggcgaaa ggctaaactg gtagaagatc gttgtgccac attggggatc
    24061 actcacttgt atcaggggca gtcaaacaaa ctgatcgcct ttagcgatct gctggaaaaa
    24121 ctggcgattg ccccggaaaa tgtggcttat gtcggcgatg atctcatcga ctggccggta
    24181 atggaaaaag tgggtttaag cgtcgccgtg gccgatgcgc atccactgtt gatcccgcgc
    24241 gccgattacg tgacgcgcat tgctggcggt cgtggcgcag tgcgcgaagt ttgcgactta
    24301 ttactcctgg cgcagggcaa actggatgaa gccaaagggc aatcgatatg agtaaagcca
    24361 gacgttgggt tatcattgtg ctatcactgg cggttctggt gatgatcggc attgatatgg
    24421 ccgaaaaaga cgataccgcc caggtggtcg tcaacaacaa tgatcccacc tataaaagcg
    24481 agcatacgga cacgctcgtc tataacccag aaggggcact aagctatcga ttgattgctc
    24541 aacacgttga atattattcc gatcaggccg tttcgtggtt tacgcagccg gtacttacca
    24601 cgtttgataa ggataaaatc ccgacatggt ccgtaaaagc agataaagcc aagctgacca
    24661 atgaccggat gctctattta tatggacacg ttgaagtcaa cgcacttgtg ccaaactctc
    24721 aacttcgcag aatcacgacg gataacgcgc agatcaatct ggtgacgcag gatgttacct
    24781 ctgaagacct cgtcacgtta tacggaacaa catttaactc cagcggtctg aaaatgcgcg
    24841 gcaacttacg cagcaagaac gccgagctga ttgaaaaggt tagaacatcc tatgaaattc
    24901 aaaacaaaca aactcagcct taatcttgtg cttgccagct cacttctggc cgccagcatt
    24961 ccggcatttg ccgtaaccgg agacactgat cagccgatcc acattgaatc ggaccagcaa
    25021 tctcttgata tgcaaggcaa cgtggttacc tttaccggta atgtcatcgt cacccagggc
    25081 accatcaaaa ttaatgccga caaagtggtc gttacccgtc cgggcggcga acaaggtaaa
    25141 gaagtgattg acggctacgg taaaccggca acgttctacc agatgcagga caacggtaaa
    25201 cccgttgaag gtcacgcttc ccagatgcac tacgaactgg caaaagattt tgtcgttctg
    25261 acgggtaatg cttatctgca gcaggtcgat agcaacatta agggcgataa gatcacttac
    25321 ctggtaaaag agcagaaaat gcaggctttc agcgacaaag gcaagcgcgt aaccaccgtt
    25381 ctggtgccgt cgcagctgca ggacaaaaac aacaaaggcc agaccccggc acagaagaag
    25441 ggtaattaat tcgttatggc aacattaact gcaaagaacc ttgcaaaagc ctataaaggc
    25501 cgtcgcgtgg tagaagacgt cagcctgacc gtcaactccg gggaaattgt cggtctgctg
    25561 gggccaaacg gtgccggtaa gaccaccact ttctacatgg ttgtaggcat tgtgccgcgc
    25621 gatgcgggca acatcattat tgatgatgac gatatcagtc tgctgcctct gcatgcacgc
    25681 gcgcgccgcg gtatcggcta tctgccacag gaagcctcca ttttccgtcg cctcagcgtt
    25741 tacgataacc tgatggcggt actgcaaatt cgtgacgact tgtctgctga acaacgtgaa
    25801 gaccgcgcga acgagctgat ggaagagttt cacattgagc acctgcgtga cagcatgggg
    25861 cagtcactct ccgggggtga acgtcgccgt gtagaaattg cccgcgcact ggctgcgaat
    25921 ccgaaattta ttctgctcga cgaaccgttt gccggggttg acccgatctc ggttatcgac
    25981 attaaacgca tcattgagca cctgcgcgac agcggcctgg gcgtgctgat cactgaccac
    26041 aacgtgcgtg aaacactggc ggtttgtgaa cgcgcttata tcgtcagtca ggggcatttg
    26101 atcgcccacg gcacgcctac agaaatctta caagacgaac acgttaagcg tgtatacctt
    26161 ggggaagact tcagactctg atagggtaga agtttgcgac gttttagcag gagagtacga
    26221 ttctgaacat gaagcaaggt ttgcaactca ggcttagcca acaactggcg atgacgccac
    26281 agctccaaca ggcaattcgt ctgttgcagt tgtcgacgct ggaacttcag caggagctac
    26341 agcaggcgct ggagagtaat ccgctgcttg agcaaatcga cactcatgaa gaaatcgaca
    26401 cccgcgaaac gcaagacagt gaaacgctgg acaccgccga cgcgctcgaa caaaaagaga
    26461 tgccggaaga gctgccgctc gatgccagtt gggacaccat ttacaccgct ggtacaccat
    26521 ccggcaccag cggtgactac attgacgatg agctgccggt ctatcagggc gaaacgacgc
    26581 agaccttgca ggattacctg atgtggcagg tcgagctgac accgttttcc gacactgacc
    26641 gcgctattgc tacctctatc gtcgatgccg ttgatgacat cggttatctg actgtcccgc
    26701 tggaagatat tctcgaaagt atgggcgatg aagagatcga catcgacgag gttgaagccg
    26761 tccttaagcg gatccaacgg tttgatccgg tcggtgtagc ggcaaaagat ctgcgtgact
    26821 gcctgctaat ccaactctcc caattcgata aaaccacgcc gtggctggaa gaggccagac
    26881 tgatcattag cgatcatctc gatctgttag ccaatcacga cttccgcact ttaatgcgcg
    26941 tcacgcgtct gaaagaagat gtgctgaaag aagccgtcaa tctgatccag tcgctcgatc
    27001 cgcgccccgg gcagtcgatc cagactggcg aacctgagta tgtcattcca gatgtgctgg
    27061 tgcgtaagca taacggtcac tggacggtag aactcaacag tgacagcatt ccgcgtctgc
    27121 aaatcaacca gcactacgcc tcgatgtgca ataacgcgcg caacgatggt gacagccagt
    27181 ttatccgcag caatctgcag gatgccaaat ggttgatcaa gagtctggaa agccgtaacg
    27241 atacgctact gcgcgtgagt cgctgtatcg ttgaacagca gcaagccttc tttgagcaag
    27301 gtgaagaata tatgaaaccg atggtactgg ccgatatcgc ccaggctgtc gaaatgcatg
    27361 aatcgacgat atctcgcgtg accacgcaaa aatacctgca tagtccacga ggcatttttg
    27421 aactgaagta tttcttttcc agtcacatca ataccgaggg cggcggcgaa gcttcctcca
    27481 cggcgattcg tgcgctggtg aagaaattaa tcgcggcgga aaacccagcg aaaccgttga
    27541 gcgacagcaa gttaacctct ttgctgtcgg aacaaggtat catggtggca cgccgcactg
    27601 ttgcgaagta ccgagagtct ttatccattc cgccgtcaaa ccagcgtaaa cagctcgttt
    27661 gacccaaccg ataaggaaga cactatgcag ctcaacatta ccggaaataa cgtcgagatc
    27721 accgaggcac tgcgcgaatt tgttacagcc aaatttgcca aacttgagca atattttgac
    27781 cgaatcaacc aggtctatgt tgttctgaaa gtggagaaag tcacccacac ctcagatgca
    27841 acactgcatg taaacggtgg tgaaattcat gccagcgcgg aaggtcagga catgtacgct
    27901 gccattgatg gcttaattga taagctggca cgccagttga ccaaacataa agataaactg
    27961 aaacaacact aattgtccgg gcaattagca tgtgcatggc ggcctgttgt gcggcacaac
    28021 gggccatttg tacggttaat gctccgagcc tgttccactg tttgagtggg caggttctta
    28081 ggtgaaatta tgacaaataa tgatacaact ctacagctta gcagtgttct taacagggaa
    28141 tgtacgcgaa gccgcgtcca ctgtcagagc aaaaaacgcg ccctggaaat catcagcgag
    28201 ctggcggcga aacaacttag cctgccgcct caggtggttt ttgaagctat cctgacgcgg
    28261 gaaaaaatgg gcagtaccgg tatcggcaat ggtattgcca ttccgcatgg caaactggaa
    28321 gaagatactc tgcgcgccgt tggcgttttc gttcagctcg aaacgcctat agctttcgat
    28381 gccatcgaca accaaccggt ggacttactt tttgccctgc tggtgtcggc agaccaaact
    28441 aaaacgcacc tgcatactct gtcgctggtg gcgaaacgtc tggcggacaa aaccatctgc
    28501 cgccgtttac gcgcagccca gagcgatgaa gagctgtatc aaatcattac ggataccgaa
    28561 ggtactccgg atgaagcgta gttattcggt aatgtctctt ttagacgttg tgaggagaaa
    28621 cagtacatgg tactgatgat cgtcagcgga cgttcaggtt caggtaaatc tgtcgccctg
    28681 cgtgcgctgg aagatatggg tttttactgc gtggataacc ttcccgtagt gttgttaccc
    28741 gatctggctc gaaccctggc cgatcgcgag atttctgccg ccgtcagcat tgatgttcgt
    28801 aatatgccgg agtcaccaga aatattcgaa caggcgatga gtaacctgcc tgacgctttc
    28861 tcaccgcaac tcctgttcct ggatgccgac cgtaatacct taattcgtcg ttacagtgac
    28921 acgcgccgac tgcatccgct ttccagcaaa aacctgtcgc tggaaagtgc tatcgacaaa
    28981 gaaagcgatt tgctggagcc tctgcgttcg cgagcggatc tgattgtcga tacctcagaa
    29041 atgtccgttc acgagctggc tgaaatgctg cgtacccgtc tgctgggtaa acgtgaacgc
    29101 gaactgacca tggtctttga gtctttcggc ttcaaacacg gtatccctat cgatgcagat
    29161 tacgtctttg acgtgcgctt cttgccgaac ccgcactggg atccgaaact gcgtccaatg
    29221 acaggtcttg ataaacctgt cgccgcgttc ctcgaccgcc acacagaagt acacaatttt
    29281 atctaccaga cgcgaagcta tcttgagcta tggttaccta tgctggaaac caacaaccgt
    29341 agctacttga cggtcgccat tggttgtacc ggcgggaagc accgttcggt gtatattgca
    29401 gagcaactgg cagactactt ccgctcgcgc ggtaaaaacg tccagtcacg ccatcgtacg
    29461 ctggaaaaac gtaaaccatg accgtcaagc aaactgttga aatcacaaac aagctgggca
    29521 tgcatgcccg gcctgcaatg aagctgtttg aattaatgca gggttttgac gctgaagtgc
    29581 tgttacgtaa tgacgaaggc accgaggccg aagccaacag cgttattgcg ctgctgatgt
    29641 tggattctgc caaaggacgg cagattgaag ttgaagcgac cggcccacag gaagaggaag
    29701 cactggccgc cgttatcgcc ctctttaact ctggttttga tgaagattaa tcttcatcac
    29761 tttgacatac aactcccttc aaactccccc cgacaataag aaaatcacgt actgaaatcg
    29821 ttctcaatca acgtcatttg tacattttgt gcgcttttca ctttcagaag aaccttaaga
    29881 aaaccttaag aggcattgtt taggttttgt ttaagttaat cgaccatact ggagatcgtc
    29941 agaaaatatt tccaggagat ggcatgattc gcttatctga acaaagtccc ctgggcaccg
    30001 gaagacatcg gaaatgttat gcgcatccgg aagatgccca acgctgtatc aagattgtct
    30061 accatcgtgg cgatggtggc gataaagaga tccgccgtga gttaaagtac tacgcgcatc
    30121 ttggtcgccg gttaaaagac tggagtggaa taccgcgcta tcacggtacc gttgagacgg
    30181 attgcggaac cggttatgtc tacgatgtga ttgcagattt tgacggcaaa ctctccatta
    30241 cgctgaccga atttgctgaa caatgccgtt acgaagaaga cattgcacaa ctgcgccagc
    30301 tactgaaaca gctgaagcgt tatttgcagg ataaccgtat cgtgacgatg tcgctgaagc
    30361 cgcagaatat cctctgtcac cgcatcagtg agtcagaggt tatcccggtc gtctgcgaca
    30421 acattggtga aagcacattg attccactgg cgacctggtc gaagtggtgt tgtctgcgta
    30481 agcaagagcg gttatggaaa cgatttatcg cccaaccggc gctggctatt gccctgcaga
    30541 aagatctgca acctcgtgag agcaaaacgc tggccctcac ttcgcgcgaa gcttaatcca
    30601 gctggtgttg ttgcataaac ggctcaccgc ctaactgata catctgccgt aaaatccacg
    30661 cctgacggct acgcacgtag cccgatggcg aggagacttt gaaacgaagt ggattaggta
    30721 atacagctgc cagtaatgca gcttccgacc gggtaagttt gctcgcgggt ttgtggaaat
    30781 aacgttgtgc cgcagcttcg acgccaaaca caccgtcgcc aaattcggcg atattcaggt
    30841 aaaccgtcag gatacgcttt ttgctccaga ctgtttctat ccccagcgtt aatccagctt
    30901 ccagcccttt tcgtacccag ctacgcccat cccataaaaa gagatttttg gccgtctgct
    30961 gagaaatcgt tgaagcaccg cgaatacggt tttcattgcg ctcgttgtgc gccagggctt
    31021 tctcaatgga agcgacatca aagccccagt gctcagaaaa tttctgatct tctgcggcaa
    31081 tcaccgccag tcccatccac ggcgagattt gatccatact gacccagtca gaatgtgcca
    31141 cgtaacgaaa attgccatgc agccaggcgc tgacctgtcg ctcgaccatt accgctgaga
    31201 agggaacagg cgcaacacta aacaacgcga tgcccccgcc ccagaaaacg gcgaggacaa
    31261 ccattaacag caaaagaaaa cggcgaacga aactaaacac cgttaagcgg cttttactca
    31321 ttcagccaga accagcacgc gggaaaccag cttatcaatg ccgctcgccg cttctgcaat
    31381 gttctgcgcc agcatatatg ctggggtggt gacaatctta ttgtcttcat caaccacgat
    31441 atcatcgaca ggacagggca catgctccgc gcccatctct tccagcactt ctgcggtatc
    31501 gatatcagta ccgatggtca aacgcagcgg gaaatcgaaa attttcggca gcatcgccgg
    31561 ggcaatacac ataaaaccaa gcggttttcc ggcctgatgc atcgcttgtg ccagcgcctt
    31621 taattcacgg tcaacggtgc attcgctacc aagagtggca aaattgctta aattcttcgc
    31681 cgcgccaaac ccccccggca caatcaacgc atccagttca gcggcatcgg cctgggccag
    31741 aggacggatt tcaccgcgcg ttattcgtgc cgcttcaatc agcacgttgc gcgtttccgt
    31801 catcgcttcg ccagttaaat ggttgataac atcaacctgc tgcttatccg gtgcaaagca
    31861 gaccgcctgc gcaccgctgc gtgaaatagc taacaacgtc aacaccgctt catgaatttc
    31921 agaaccgtca tagacgccgc atccgctcag aattacgcca attttcttca ttgtgatcat
    31981 cctttcgcaa ttgactgaaa cacattaatt tttttaataa aaatggtacg catcacacat
    32041 ttaactgatt catgtaacaa atcatttaag ttttgctatc ttaactgcgt gcggcctgaa
    32101 aaacagtgct gtgcccttgt aactcatcat aataatttac ggcgcagcca agatttccct
    32161 ggtgttggcg cagtattcgc gcaccccggt ctagccgggg tcatttttta gtggcttttg
    32221 ccacccacgc tttcagcact tctacgtcgt gacgccactc ttctttcatc tcttcaatcc
    32281 attcaccgac gttatcttcc caggcaggaa ggtcaggaga ctgaatttgc tgacccagct
    32341 gttgcagatg gcgtaacccc actgaccccg ccgcaccttt aattttatgt ccttcctcaa
    32401 caatgccttt tttatcctgc gccgtcagat tcgactccag cacgctgaca tagcccggca
    32461 tcatcttctc aaacaccgct aacccgtcgg tgatcagctt cggtcctaca agttcgagat
    32521 actgttccag catgggaata tcgagcaatg cttctgattt actgttctct tctgtcgtca
    32581 ccgtactctc ctcatcatcc tgggtatccc agaatttctt gatcatcgcg gttagcgccg
    32641 gaacagaaag cggcttactc agcacatcat ccattccagc attgaggtac tcttgtttgt
    32701 ctttcagcac gttagcggtt aaggccacca gcggcggtaa atcctctcgc ggataacgct
    32761 tcgtcagttc acgagagata tccagcccgg tcatatctgg caactgaata tccagtaaca
    32821 ccaggtcgta ttcgcccggt ttaaacatct ctagcgccgc cttgccggtc atggcgacat
    32881 caacgctgtt acctaatttt tccagcacag aacgcgcaac aatcacgttc agttcaatgt
    32941 cttccaccag cagcacattc agcgccggta aaggcatatc gtcttcatca aacgcatcat
    33001 cgacctcttc tgctaccgac ggtgcgtgga tcgtcaacgt aaaggttgaa cctttgccct
    33061 gttcgctggt aaccgtaata tcgccgccca tatttttcgc cagacgacga gaaacggcca
    33121 gaccaatacc ggtgccggtg gcaggtttac cgccatgact gtctttcacc tggtaataca
    33181 tggcgaaaat tttatccagc tcatcctgcg gaatgccgat accagagtct tccacttcaa
    33241 aatgcagcat atcgccttca tcgtagcgca cgcgcacggt aacctggcct tgctgggtga
    33301 atttgacggc gttactgatg aggttccaca ggatctgccg taaacgcgtc ccgtcggtaa
    33361 tgacctgatg cggtaatggc agcgtcggct ccaggttaaa gcgcaatcct ttttgttgcg
    33421 cctgcaatgc ggagagattt tccagatcgg caaggaagct ggtgaaatca accggttgat
    33481 tatcaagctg gaccttgcgc cgttccatct tatccatgtc gataatatcg ttaaagatat
    33541 tccccagcgt gacggccgaa acatggatgg tcttgagata tttttcctgc tcggcggtga
    33601 gttcggtatc cagcagaatg cggctcagac cgacgatacc gttcagcggt gtacgcaatt
    33661 cgtgactgat ggtggagata aacgtcgttt tgtcgcggct ggcccgttca agcgcatcct
    33721 gataccgctt acgctcggta atgtctcgac caaagcccat caaaccgtga cgtttaccca
    33781 cgcggtcgta gtacggcact ttacggattt caaagcaggc tttgcgcccg tccgggtaat
    33841 ccagccactg ttcataggtc agtgacacat tatgacggaa cactttttca tcggtttcaa
    33901 tgacttttgc ggcggcttcc ggtgagtaaa catcaacagg tttcaggtga accagttgtt
    33961 tttcgctttt tccggtcagc agctccatcg cgcggttaca gccggaaaac tctttatctt
    34021 cgttacgata aaaaaccagg tcgggtgaag catcaaggaa ggaacgtaag aatgaggatt
    34081 gctgctcgag ctgaatttgt gtctcttcgc gctctttgat ttcaattttc agttggccga
    34141 aggtttcctg cagttctgct tccgcttttt cacgaacggc gatttcctga tttagctggg
    34201 caatattatc ttttaactga acgttgaggc tcaaatcgcg ctcgcgcatc tcctccagtt
    34261 tttgcaccag ccgtgacaga cgttgtcgtg actcctccag ttgctcgacg accaccgata
    34321 gaaagtagac cgcccacggc gtaatcaaca aaccaaagaa gatagaacga ataacatcaa
    34381 tgctttcgac ctgaccatgc agcaccatgg ttaccgccat ttgtaccaca atggcaagaa
    34441 cgacgagggc cagcgccagc aacattgaga agcgcaccag acctaacttc atcatcaggt
    34501 caacataata ctgcgccagc agacgaattt gcttcattag ggaattcctt cacgacaacc
    34561 tgccccaata atacccaatt ctgacagtta cgttataaat tgtgtgagaa atgcggaatt
    34621 cccctcaacg acctactccg ttggaggtag ccacggacgt cccagcgccg atccctgtac
    34681 gccatgttca ttcagatagc gatccagctc gaccattccc gtccatcgat tttcgcacca
    34741 cagaggagca agcagcgtcg ggcgacgggc gctggcggaa atgcggtggt agatcacttc
    34801 cggtggcgta tggcgaatca tttctccggc agtgagcgtg taatcctcca gttcaatacc
    34861 gttcaaacgc cccgcttccc aggctttcgc cataatgctg cctttcacaa tatgcaacgg
    34921 atgtagcttt ataccgtcaa caccggtttc cactacccgc tccagcgttt gcagacattc
    34981 agccagccct tcgcctggca gaccgacaat taaatgcgag cacaccttca gcccgcgctg
    35041 gcgtgccagc tgcgtcgtgc gctgataaca ggcgaaatca tgaccgcgat taatgcgatg
    35101 tagcgttttg tcgtgcacgg tttgcagccc cagctccagc cacacttcgt agccctggtc
    35161 tttatattcg caaagcagat ccagcaccgc atccggcacg cagtccgggc gcgtgccgac
    35221 acacaaaccg acaatattgg cctggctcac cgcctgttga tacatcgaac gcagcacctg
    35281 aacttcggca aaggtgctgg tatacgcctg gaaataggcg aggtaacgtt tcgcgcgatt
    35341 aattaaattc gcctgatgcg ccagctgttc ggcaatggaa cgatgctgct gcgcttcgtc
    35401 ggcaaacgag gcaacattac agaaggtgca gccgccgcgc ccgatggtgc cgtcgcgatt
    35461 cgggcaactg aaaccgccgt gcagcgtcag cttatgcacc ttttgcccat aacgacgggt
    35521 gagatcacca ccaaacatat tgactaattt ctgtaactgc ataatctgat agaccgcgcc
    35581 ttgaaaagag gccaaagcct gccattttta gctcaattcg gcgatgacct ggatcaatcg
    35641 tcccggcccg cttttatcaa ctgcataatc aatcaaaatt accgaaattt catgcataat
    35701 cacataaatc acttttgctt atcttgtgtc agattttttt atctccttat ggattttagg
    35761 caaaaacagt agcatgaaac gtcattacca attaatgcag tataaaatgc tggttttctc
    35821 gtcaattcaa ggcaggataa gggttaacac gcctttatga cagtcaggaa ttgactgttt
    35881 ctctaacgac ttccctttta gccttaaaga taaaatctat tttaatttca gacatttaat
    35941 aaagaatttt gcgctaaagc acatttctgt accaataagc ttgccatttg acctgtatca
    36001 gctttcccga taagttggaa atccgctgga agctttctgg atgagcagcc tgctcatcat
    36061 atttatgcag taattgagat cccctcttca ccgtattaac cgatgcgaaa aggacaacaa
    36121 gggggcgaat gcgaggcgcg cgtatgacac gcaacccccg tcgccacgct ctttctgtac
    36181 ccgtgcgcag cggttcggta gtggggttcc cgcagagcct gggggaggtt cacgatatgt
    36241 tgtacgataa atcccttgag agggataact gtggtttcgg cctgatcgcc cacatagaag
    36301 gcgaacctag ccacaaggta gtgcgtactg caatacacgc actggcccgc atgcagcacc
    36361 gtggcgcgat tctcgccgat ggtaaaaccg gtgacggttg cggcttgctg ttacaaaaac
    36421 cggatcgctt ttttcgcatc gttgcgcagg agcgcggctg gcgtttagcg aaaaactacg
    36481 ccgtcgggat gctattcctg aataaagatc ctgaactcgc cgctgccgca cgccgcatcg
    36541 ttgaagaaga actgcaacgc gaaaccttgt cgattgtggg ctggcgtgat gtccccacta
    36601 acgaaggcgt actgggtgaa atcgccctct cctctctgcc ccgcattgag caaatttttg
    36661 tgaacgcccc ggcaggctgg cgtccgcgcg atatggagcg ccgtctgttt atcgcccgcc
    36721 gccgcattga aaagcgtctc gaagccgaca aagacttcta cgtctgtagc ctatcgaatc
    36781 tggtgaacat ctataaaggt ctgtgtatgc cggcggatct gccgcgcttt tatctggatc
    36841 ttgcggacct gcgtctggaa tcggccattt gcctgttcca ccagcgcttc tccactaaca
    36901 ccgtaccgcg ctggccgctg gcgcaaccgt tccgctatct ggcgcataac ggtgaaatca
    36961 acaccatcac tggtaaccgc caatgggcgc gtgcgcgtac ctataaattc cagacgccgc
    37021 ttatccctga tctgcacgac gccgcaccgt tcgtcaacga aaccggctct gactccagtt
    37081 cgatggataa catgctggaa ctcctgctgg caggcgggat ggatatcatc cgcgccatgc
    37141 gtctgttagt gccacccgcc tggcagaaca acccggatat ggacccggaa ctgcgtgcct
    37201 tctttgactt taactccatg catatggagc cgtgggatgg cccggcgggc atcgtgatgt
    37261 ccgacggtcg ttttgccgcc tgtaacctcg accgtaacgg tctgcgtccg gcgcgctacg
    37321 tcatcaccaa agataagctc atcacctgcg cctctgaagt cggtatctgg gattaccagc
    37381 ctgacgaagt ggtcgaaaaa ggccgcgtcg ggccaggcga actgatggtt atcgacaccc
    37441 gcagtgggca tattctgcac tcggcagaaa ccgatgacga tctgaaaagc cgccatccat
    37501 ataaagagtg gatggagaaa aacgtccgcc gactggtacc gtttgaagat ctgcccgatg
    37561 aagaagtggg tagccgcgaa ctggacgacg acacgcttgc cagctaccag aaacagttta
    37621 actacagcgc ggaagagctg gactccgtaa ttcgcgtact gggcgaaaac ggtcaggaag
    37681 cggtcggttc gatgggcgat gataccccat tcgccgtgct ctccagtcag ccgcgcatta
    37741 tttacgacta cttccgccag cagtttgccc aggtgactaa cccgccaatc gacccgctgc
    37801 gtgaagcgca tgttatgtcg ctcgccacca gtatcggtcg tgaaatgaac gtcttttgcg
    37861 aagcagaggg ccaggcgcac cgtttaagct ttaaatcgcc gattctgctc tactccgatt
    37921 tcaaacagct cacgacgatg aaagaggagc actaccgcgc agatacgctg gatatcacct
    37981 ttgacgtcac taaaaccacg ctcgaagcga cagtcaaaga gctgtgcgac aaagccgaaa
    38041 aaatggtacg tagcggcacc gtgctgctgg tgctctccga ccggaatatc gctaaagatc
    38101 gcctgccggt tccagccccg atggcggttg gcgcgatcca gacccgtctg gtcgatcaaa
    38161 gcctgcgttg cgatgccaac atcatcgtcg aaaccgccag cgcccgcgat ccgcaccact
    38221 tcgccgtgtt gctgggcttc ggcgcgacgg ctatttatcc ataccttgcc tatgaaacgc
    38281 tgggccgcct ggtagacacc catgcgattg ccaaagatta tcgtaccgtg atgctcaact
    38341 accgtaacgg catcaacaaa ggcttgtaca aaatcatgtc caaaatgggc atctccacca
    38401 tcgcctctta ccgctgctcg aaactgtttg aagcggtcgg tctacacgat gatgtagtgg
    38461 gcctgtgctt ccagggggcg gtcagccgca ttggtggggc aagctttgaa gacttccagc
    38521 aggatctgct gaatctgtcg aaacgtgcct ggctggcgcg taagcccatc agccagggcg
    38581 gcctgctgaa atacgtccac ggcggcgaat accacgccta caacccggac gtggtgcgca
    38641 cgctgcaaca agcggtacaa agcggcgagt acagcgacta tcaggaatac gcgaagctgg
    38701 ttaatgagcg tccggcaacc acgctgcgcg atctgctggc aattacgccg ggtgaaaacg
    38761 cggtcaacat tgctgatgtt gaaccggcaa gcgaactgtt taaacgcttc gataccgctg
    38821 cgatgtctat cggcgcgtta agcccggaag cccacgaggc gctggcggaa gcgatgaaca
    38881 gcatcggcgg taactcaaac tccggtgaag gcggtgaaga tccggcgcgc tacggcacca
    38941 acaaagtgtc gcgcatcaag caggtggctt ccggtcgctt tggggttact ccggcgtatc
    39001 tggtcaatgc cgacgtcatt cagattaaag tcgcccaggg cgcgaagccg ggcgaaggcg
    39061 gtcagttgcc gggtgataaa gtcactcctt acatcgccaa actgcgctat tcggtgcccg
    39121 gagtgacgct gatctccccg ccgccgcacc acgatatcta ctctatcgag gacttagcgc
    39181 agctcatttt cgacctcaag caggttaacc cgaaagcgat gatctccgtg aagctggttt
    39241 ccgaaccggg cgtaggcacc atcgcgactg gcgtggcaaa agcttatgcc gatttaatca
    39301 ccatcgcagg ctatgacggt ggcaccggcg caagcccgct gtcgtcagtg aaatacgcag
    39361 gttgcccgtg ggagctgggg cttgttgaaa cccagcaggc gctggttgcc aatggtctgc
    39421 gtcataagat ccgtttgcag gtcgatggcg gcctgaaaac cggtgttgat atcatcaaag
    39481 cggcgattct cggcgcagaa agcttcggct tcggcactgg cccaatggtc gcgctcggct
    39541 gtaaatatct gcgtatttgc cacctgaaca actgcgcaac gggtgtggca actcaggatg
    39601 acaaactgcg taagaatcac tatcacggcc tgccgttcaa ggtgacgaat tactttgagt
    39661 ttatcgcccg tgaaacccgc gagctgatgg cacagctggg ggtaacgcgg ctggtggatc
    39721 tgattggtcg caccgacctg ctgaaagagc tggacggttt caccgccaaa cagcagaaac
    39781 tggcgctgtc gaagctgctg gagaccgccg aaccgcatcc aggtaaggca ctctactgca
    39841 ccgaaaacaa cccgccgttt gataacggcc tgctgaacgc gcagttgctg caacaggcga
    39901 aaccgtttgt cgatgaacgc cagagcaaaa ccttctggtt cgatatccgc aataccgatc
    39961 gctccgtggg cgcgtcgctt tcaggctata tcgcccagac gcacggcgat caggggctgg
    40021 cagccgatcc tatcaaagcg tacttcaacg gcaccgcagg ccagagtttc ggtgtatgga
    40081 acgcgggcgg cgtggaactg tacctgaccg gggatgccaa cgactatgtc ggtaaaggca
    40141 tggcgggcgg cttaatcgcc attcgtcctc cggttggttc cgccttccgc agccatgaag
    40201 caagtattat cggcaacacc tgcctgtatg gcgcgaccgg tggtcgtctg tatgccgcag
    40261 gccgcgcggg tgaacgtttc ggcgtgcgga actccggtgc tatcaccgtg gtagaaggca
    40321 ttggcgacaa cggctgtgaa tatatgacgg gcggtattgt ctgtattctg ggtaaaaccg
    40381 gcgttaactt cggtgcgggc atgaccggcg gcttcgctta cgttctcgat gaaagcggtg
    40441 atttccgcaa acgcgttaac ccggaactgg tcgaggtctt aagcgttgac gatctggcga
    40501 tccatgaaga gcatctgcgc ggtcttatca ccgagcatgt tcagcatact ggctctcagc
    40561 gcggtgaaga gattctggcg aactggtcaa ccttcgccac taaatttgcg ctggttaaac
    40621 cgaagtccag tgatgtaaaa gcactgctgg gtcatcgtag tcgtagcgca gcagagttgc
    40681 gcgtgcaggc gcagtaaggg gtagcaacaa tgagtcagaa tgtttatcaa tttatcgacc
    40741 tgcagcgcgt tgatccgcca aagaaaccgc tgaagatccg caaaattgag tttgttgaaa
    40801 tttacgagcc gttttccgaa ggccaggcca aagcgcaggc tgaccgctgc ctgtcgtgcg
    40861 gcaacccata ctgcgagtgg aaatgtccgg tacacaacta catcccgaac tggctgaagc
    40921 tcgccaacga ggggcgtatt tttgaagcgg cggaactgtc gcaccagacc aacaccctgc
    40981 cggaagtttg cggacgagtc tgcccgcaag accgtctgtg cgaaggttcc tgcactctga
    41041 acgatgagtt tggcgcggtg accatcggca acattgagcg ttatatcaat gataaagcgt
    41101 tcgaaatggg ctggcgtccg gatatgtccg gcgtgaagca aaccggtaaa aaagtggcga
    41161 ttatcggcgc aggcccggca ggtctggcgt gtgcggatgt cctgacgcgt aacggcgtaa
    41221 aagccgttgt cttcgaccgt catccagaaa ttggcgggct gctgaccttc ggtattccgg
    41281 ccttcaagct ggaaaaagag gtaatgacgc gtcgccgtga aatcttcacc ggcatgggta
    41341 ttgaattcaa actcaatacc gaagtgggcc gcgacgtgca gctggacgat ctgctgagtg
    41401 attacgatgc cgtcttcctt ggcgtcggga cttatcagtc aatgcgcggc gggctggaaa
    41461 acgaagacgc cgatggcgtg tacgcagcgc tgccgttcct tatcgccaac accaaacagt
    41521 taatgggctt tggtgaaacc cgcgacgaac cgttcgtcag catggaaggc aaacgcgtgg
    41581 tggtccttgg cggtggcgac actgcgatgg actgcgtgcg tacgtccgtg cgccagggag
    41641 cgaagcacgt tacctgtgcc tatcgtcgtg atgaagagaa catgccgggt tcccgccgcg
    41701 aagtgaaaaa cgcgcgggaa gaaggcgtag agttcaaatt caacgtccag ccactgggga
    41761 ttgaagtgaa cggtaacggc aaagtcagcg gcgtaaaaat ggtacgtacc gaaatgggcg
    41821 aaccggatgc caaaggccgt cgccgcgcgg agatcgttgc aggttccgaa catatcgttc
    41881 cggcagatgc agtgatcatg gcgtttggtt tccgtccaca caacatggaa tggctggcaa
    41941 aacacagcgt cgagctggat tcacaaggcc gcatcatcgc cccggaaggc agcgacaacg
    42001 ccttccagac cagcaacccg aaaatctttg ctggcggcga tatcgtccgt ggttccgatc
    42061 tggtggtaac cgctattgcc gaaggtcgta aggcggcaga cggtattatg aactggctgg
    42121 aagtttaagc tctgtcccag cgccgcctcc tcagaaaaga gtcggcgctg aaattctcct
    42181 ttctttctgg aaagcgcctc gcataatctg aagaaaacca atgtaatacg ctcatctgga
    42241 ttgttaccgg tagatgcccg gcgtatagct gattccatta ttcaacgcag cgccatataa
    42301 agggacagat aatggaatcg ctctcagaga gaacctcaac aggctaccag caaatccacg
    42361 acggtattat tcacctggtc gatagcgccc ggacggaaac ggtacgtagc gttaacgcgc
    42421 tgatgaccgc gacatactgg gaaattggcc gacgaattgt cgaatttgaa caaggtggcg
    42481 aggcaagggc tgcgtatggt gcgcagctaa tcaagcgact atcaaaggat ttaagtctaa
    42541 ggtataagcg tggtttctct gcaaaaaact tacgccaaat gaggcttttt tacctctttt
    42601 ttcaacatgt tgaaattcgc cagacagtgt ctggcgaatt aacaccattg ggaattccgc
    42661 agacaccatc tgcagaattc ccatcggcaa agatttggca gacgctgtct gccaaattga
    42721 gaggctgttt gatatttcca cagcttttgt attgcatctc ctattttcgg tttgtatatt
    42781 catgcaaata aacgaaggaa agttgccgtg atttacattg aacagcttga attaatccat
    42841 aaaagtggtg atgtgttgta tccagtaaaa ataaccagaa aatcatcagg aaaaactgcc
    42901 ttccaccttg ttccattcgg cttaaataag actcacgacc ttctagaggt ggaagatgct
    42961 tcagaagcaa ttcggctggt gattgatgaa cgtcattcca ttcgctgcag tacacttaca
    43021 gcaactatta cgaataaaaa gggcaagcga attaagcgga caggtatata cagtatcaag
    43081 ggtgttaaca taaaagaata caacgtcagg taatggcaca gagcggtccg tcagttcagg
    43141 tttggctctg tgccaaagat gggtcaggtc acacctgatc taataaattt aagccattaa
    43201 atcagcctta accttcacca ccaccttttt aatctcaccc ggctcgccaa caacgcatcc
    43261 cggcttatgc ggctctccta gcataaacac ggcgaacatt cccggtttca ggatgatggc
    43321 ttgctcgttt tcaatggtgc tgcaaagctg gtaatcatcc tcatggtgga actcttcaca
    43381 ctgacgcgca gtgcctgcca tgccaaacag aatccgttct tcaccgttta ataacagctg
    43441 gatatcaatg tattgctcgt gcagctccgc ttttttctca acgggcgatt gagtgttaaa
    43501 cgtcatgaca ttcataaaaa tattgtcgcc ctgtaattcg taacgacccg gcgctttttc
    43561 ttgcggtctg gcagctaatg ccagcgttaa cgcgtcctgt aacgcaggat gtaacccagc
    43621 agacggtaat gaccgtactt cacccatcat cataattttt ctccctgggc caacagcgct
    43681 gccccaagta aacctgcatc atggcggtag tgcgccgcca gtaaatcaac atgaaatacc
    43741 gctggctcct gcgccagata cgtttccacc aacgccagat acccttctgc cagaccaacg
    43801 ctgccaccga ccaccacgca ctggcaatca gttgtggctt taatatcagc gatcagcctt
    43861 gccagcacat gtgcggagcg gtgaatcagt tgctgcgcct gctcgtcgcc ctgcccggcg
    43921 cgtgtgaaaa tagttctcgc atccgcgccc atgagttcac cttgcgctgc cgctgcaata
    43981 ccgcgaccag aagcaatcgc ttccacgcaa cctgtgcgtc cacagccgca gactgggccg
    44041 tgtggatcgg caagcgtatg cccgatatgc cccgccagac cgccagggcc ggtaagcagt
    44101 ttaccgccgc tcactacacc gccgccgacg ccagtggaaa cggtgataaa caccatctcg
    44161 gttacatcgc cttccagcgc ctgatactct gcccatgctg cggcctgcgc gtcgttaatg
    44221 gcaatggtcg gcaaattggt aagttgttcc agcgttttga ctaacggaaa gtgtagcaat
    44281 ccaccgagat tatgcggatt cagcgccagc aaactgccgt cgcgaataat tccggttgaa
    44341 gcgatggcaa cccgctgcgc atgagcttgc aacggagaga ctaatgcgga taaggcatca
    44401 cgcaaggctt ctggtgtctg gctggctggc gtaggaagtt cacgacgatc gcggatctgc
    44461 ccgtcagcgc caatcagcgc ggcggcaagt ttagtaccgc cgatatcaat cgccagtgtg
    44521 gtcatagcac cgcctttttc atcgctgtgt tgtaccactg acaaatgtgt tcaagacgcg
    44581 taatggcaga accgaccgtc accgcccacg cgccgtggcg catcgcatct gccgcctgag
    44641 caggcgtgtt gtaacgccct tcggcaatca cccgacatcc ggcttcgctc aacgttttca
    44701 ccagcgccag atccggctct tctggcgttt caggcgtggt atagccagaa agcgtagtgc
    44761 caataatttc ggctcccagc ttttggcatg ccaggccgtc ttccggcgtt gagcagtcgg
    44821 tcatcgccag taaaccgtga tgatgtatac gtgccagcag cgtttcaaca ggcaccggac
    44881 gcgggcggtc ggtgccgtca atggcgataa tgtccgcgcc cgcctgcgcc agcgcatcga
    44941 catcttcaat ataggccgtg atgcgtaccg gagaatcctc cagatcgcgt ttcacaattc
    45001 caataatcgg cacgctcacc accgcacgcg tggcttgcag atttgccaca ccttcgatgc
    45061 gaatggcaac cgcgcccgcc tgctctgccg ctaatgccat ggctgcgacg atttcgggtt
    45121 tatcgagcgg gctgtcagga atcggctggc aggagacaat caggccaccg ttagcagcga
    45181 ttttttgatc cagttgtgca agtaacgaca tacatcttcc cttagcgaaa ggcccggtac
    45241 atagaccggg caacaggatt aacttttggt tttgactaaa tcgtttttgg cgctgccaaa
    45301 tggcacggca ccgctaaatg gtttgccgtc gatagcgtca tgagtacgca atgcttccgg
    45361 gcgcagccaa cgctgaacgc gggaaggcat atccagacca atcagcagga tcaccacaaa
    45421 tgtcaggctg aacgagagcg atgccagcgc agtacccaga tccagacgtt gagcgatcaa
    45481 cgcgccgatg attggggcca gtgcgccacc caatgcgcca acgttgtagg taaagcccag
    45541 gcccgctgca cgctggtcgg tatcgaaata accgccaatc agtttcggta agatcccggc
    45601 gatcccttgt ccaagcattt gctggaagaa cagtaacaga ccgagcaccc agacgtttgc
    45661 gccgccaatc gcaaataccg gaataatcag cagctgcgag gccagcaggc tacaaacgta
    45721 cgctttgcgg gttcccagcc agtcaccgag gaagcctcct acgcagcatc ccacggccgc
    45781 gccaaagcca ctaaagaaca gcacattggc gacagtatgc gggttataag ccagatcggt
    45841 tttcagatac gttggcagca gcgcctgaat cggccatgag tagaggaaag caaacaacac
    45901 gaccaccatc agcattacgc ccgttggcca gcgtttgcct gtactctgca ccataaagct
    45961 gataaagatt gcggcgcata acagcccaag aacagcgacg atcgcggcat tttgcaggtt
    46021 accggcgaag cagaaccaca gcgcagtagc cgccgccagt gtcattacga tattggcaat
    46081 gcgatgttca ccacggtaga gaatatccac cattgtgcgt actggtgctt tacctgcgtg
    46141 tttctctttc cagtcttccg cttccgggat gtttttacgc agccagagag caaagatgat
    46201 tggcaaaatg ccgataaaga acagcgcacg ccaaccccag accggaacca ccaggctata
    46261 gacctgagcg gcaacgacgg cccccacaga gaagcctgaa atcaaaaaac cactggcttt
    46321 gttacgcaga tgttttggcc agctttcaat gacataggtg gcgctggaac cgtattcacc
    46381 cgccatcccc atgccgatga ccagacgagc gataaacatg gtgatgtagc ctggcgcaaa
    46441 gccgcaggcc agcgtcccgg ccgagaagag aacgatgctg gtgaccattg ccagacgacg
    46501 cccgtagcgg tcacccatag cgccgagcat caggccgccg aaccagcgag agataaaggc
    46561 tgcggagatc agacttgccg cctgcaccgt cgtcagcccg aattcaccct gtacttcggt
    46621 gagtaccagg gcgattaaaa cgaaatcaaa accgtcaagc agatatccca accaggcagc
    46681 ggaaaatgcg cgccattgtg cacggttgag atggcgatac cacgggatat tctgggttgt
    46741 agtactcatt gtgagtctcc cgcggtgggc gatgcccaca cgctttggta tgaaaattgt
    46801 agggtacaga tgcgtttatt tcccctcacc cggtaggggc gagcgagggg aaaacaactc
    46861 acccgcgctc ttgcatcaac tgctgggcca gcgccttcag ttctggcaga tatttttcat
    46921 ctaccggtcc aaacggtttg cggcacagcg gcacagaaac gacatccata taatgcagga
    46981 cagttttcag gccgcggaat acgccagttt tgatcagtaa atcaatgact ttgttgcact
    47041 cagtttgcag tttctgcgcg gtctggatat cgccttcttt cagcgccttc acgatcccct
    47101 gatatcgcca gcccatgatg ttgtaagtac tgccgatacc accatcagcg cctgccagca
    47161 agccggaagc gaagatttcg tcgtaaccgt tatagagcac cagatcagga tgttcacgac
    47221 ggatctgttc catctgatag agatcgccag aagtctgttt cagcgcaccg acgccaggca
    47281 acgtaaccag ggtgttgatc tgatccaggg tcagttttac cccactcagg gctggaatgt
    47341 tgtacacaac catcggcaaa ccatccgctg aatcaataat tgcccgatag tgatcgcagt
    47401 gttcttcaaa gctgaaagga tagtagaacg gcgtgacggc ggagacggca tcgaagccat
    47461 aacgtttagc cgatgccgca agttgttggc tttcggcggt gctgacgcaa ccgacgtggg
    47521 cgatgagttt aatcttacct ttcgcctctt cggcgacgat ttccagtacc tgttcacgct
    47581 cggaaaggct ttgtacaaag gcctcgccgg tcgaaccacc cacgtataaa ccgtcgatgc
    47641 cctgctgaat attgaactga accaggcgac gcagactcgc tttatccagt gcttgttgtt
    47701 ggtcaaaagg agtcaggagt gcagccatta cgccacgtaa attcgttgcc ataaatacct
    47761 ctgaagtgat acttgtctga taaacgatat acctttatac ctgttatacc agatcaatta
    47821 agcaacaccc catacagaaa gcttataatg cgatctgctt cactaaagtg gcattatttc
    47881 tttttgttgg tggtctgacc gaaagcgtgc caggtagcag agacgctgtt gagatgcgat
    47941 tgcaacgcac gatcggcttc gtcaggatca tgacggcgga tcgcatcaac gatcgcaata
    48001 tgctgttgat agctaacgtt gttatgctcg tgcagagcct gatcagccac ggttggacgt
    48061 gcggcgatca gccagtcgag cagggcaacg tggatcgcca tgaagattgg gttaccaggg
    48121 atctccgcca gcacgcggtg gaaatcaacg tctgaacgaa tgaatgccgc gttgttatcc
    48181 agcgactgac tgttgatttc cagtgctttt gccagcaaat cgatttgctc atcggtggca
    48241 tgttcagccg catagcgcac cagactggat tcaaagaaca gacgtaattg ttcgaaatgg
    48301 gcaatcccac cgggatgaga aaggaaatct ttcgccatgc cggaaaactc accgatgata
    48361 gtgtccgcag aaggacgcga gacgcgagcg cgttcgccgt tgtttatttg caccagacct
    48421 ttgcgtttta acgctgccag cgcttcgcgc acagaaggac gcccgacgtt aaagaacgcc
    48481 atcagttcgc gttcagacgg taattgttca ccttcgccaa attcacgacg gcggatcatc
    48541 tgttccagct cttcttccac catttcggag agttttttac gcgccagcgg gcggctacgc
    48601 aagttgcgac caattgcagg tgaagaatct tcggtttgcg aatcaaatgc gttcataagg
    48661 cccattctgt aaggtcagtg tgattaacat catcagtgac atcctatcac aggattgaaa
    48721 gtaggggaaa atggcaggtt tttctctttg tgcctcatca ttaccataat taacggaata
    48781 attaactatt gcgaaaaatt aatgtaacgc agataaacac atcccgattg aattattcat
    48841 aagactattc acaagcatta tgaatattat gaatgtgttc ttacaaaata atcataagcg
    48901 catatttttt aatgaaaaat cacatcacct acaattaaaa acacgacatc cgcaccataa
    48961 atagacttgc aaaaaatata acatcattgt tttcaatctg ccgtttatgg gattgaccgt
    49021 tttcttttga cacggagttc aacaatgttc ggcataatta tatctgtcat cgtattaatt
    49081 acgatgggct atttgatcct gaaaaactac aaacctcagg tggtgctggc tgccgcaggt
    49141 gtcttcctga tgatgtgcgg agtctggtta gggttcggtg gtgtactcga tcccgccaaa
    49201 agcagcggct acttgatcgt cgacatttat aatgaaatcc tgcgcatgct gtccaaccgc
    49261 attgccggat tggggctgtc gattatggcg gtgggcggtt atgcccgcta catggagcgc
    49321 ataggagcca gtcgcgcgat ggtgagcttg ctaagccgcc cgttaaaact cattcgctcg
    49381 ccgtatatta ttctgtcggc aacttacgtc atcggccaaa tcatggcgca gtttattacc
    49441 agcgcctccg gtctgggtat gttgctgatg gtcaccttat ttccgacgct ggtgagtctg
    49501 ggagtaagtc gcctctctgc ggtggcggtt atcgcaacca cgatgtccat tgagtggggg
    49561 attctggaaa cgaactccat ttttgcagcc caggtcgcgg gaatgaaaat tgccacatac
    49621 ttcttccact accagcttcc ggtcgcctct tgcgtcatta tctcggtggc gatctcccac
    49681 tttttcgtgc aacgcgcttt tgacaaaaaa gataaaaata tcaatcacga acaggcagag
    49741 caaaaaactc tcgataatgt cccgccgctc tattacgcca ttttacctgt gatgccgtta
    49801 atcctgatgc tcggctcgct gttcctcgcc cacgtcgggc tgatgaagtc agaactgcat
    49861 ctggtggtgg tgatgttact aagtttgacg gtgacgatgt ttgttgagtt cttccgcaag
    49921 cataacttgc gcgaaacaat ggacgatgtg caggcgtttt ttgacggcat gggtacgcag
    49981 tttgccaacg tggtaacgct ggtggtcgcg ggtgaaatat ttgcgaaagg cttaacgacg
    50041 attggcactg ttgatgcggt tatcaggggt gcggagcatt ctggtctggg cggtattggc
    50101 gtgatgatta ttatggcgct agtcattgcc atttgtgcca ttgtgatggg ctctggcaat
    50161 gcgccgttta tgtcatttgc cagtcttatt ccgaatatcg cagccggact acatgtacca
    50221 gcggttgtaa tgattatgcc gatgcatttt gccacgacgc tagcgcgcgc ggtttcgccg
    50281 attactgcgg tggtgaagcg gacagcgatc cccatggcag tcggtttcgt ggtgaatatg
    50341 attgccacaa tcacgctatt ttattaagtc attaaaaaga caaaacaggc cgcctgggcc
    50401 tgttttgtat tacttcacaa cacgtaatgc cggtcgacca ccgcgtggtg gctgcggagg
    50461 ttcatcgtca ggatgagtgt catcatcgtg atctggcttg tcgccatcaa taaccgacat
    50521 aacggtttcg ttgtctgccg atgcctcttc atcattcatg atgctggtat cttcatcgta
    50581 ggcagcttca ggctcaaaca tcgtgcctgc gccattttca cgggcgtaga tagccagcac
    50641 ggcagccagc ggcacagaaa cctgacgcgg aatgccacca aagcgcgcgt taaagcgcac
    50701 ctcatcattc gccagttcca gattgccgac agcacgcggc gcaatgttga gtacgatttg
    50761 cccgtcacgc gcatattcca taggaacctg cacgccaggg agcgtcacat ccaccaccag
    50821 gtgcggcgtg agctggttat ccagcaacca ctcatagaat gcacgcagca gatagggacg
    50881 acgtggtgtt agctgtgaca aatccataca gattaactcc ggcccagacg catttcacgt
    50941 tctgcttcag ttaaagaagc aaggaaagag tcacgctcaa agacgcgggt catatagcct
    51001 ttcagctctt tcgcacccgg gccgctgagc tcgataccca gttgcggcag acgccacagc
    51061 agcggagcaa gatagcaatc gaccaggctg aactcatcgc tcaggaagta cggcttctga
    51121 ccgaagaccg gcgcaatcgc cagcagttct tcgcgcagtt gcttacgtgc ggcatctgct
    51181 tcagaagctg aaccgttgat gatggtgttc atcagcgtgt accagtcttt ttcgatgcga
    51241 tgcatgtaca gacggctttc accgcgagct accgggtaaa caggcatcag tggcggatgc
    51301 gggaaacgct catccagata ttccataatg atgcgagatt cccacagggt cagctcacga
    51361 tccaccaggg tcggaacgct ctgattcggg ttgaggtcaa tcagatcctg aggcggattg
    51421 tccttttcca cgtgttcgat ctcgaaactt acacctttct cagccagcac aatgcggacc
    51481 tgatggctat agatgtcagt aggaccggaa aacagcgtca ttaccgaacg tttgttggca
    51541 gcgacagcca tgaaaacctc caggtatagt cagaattttt actgctacca gccaccaggt
    51601 ggccagtcag aagttgtgtt acccaataag gaacgactct ctttgttcga aaatcaaaca
    51661 aaaaatgagc aatacccgac atttgggcag aaaattggat gatagtttac cagattttgc
    51721 gaccattgtg gtgagtggat gccggaaatg gggaaaaaga gatgcgcttt agtctgaaat
    51781 agttgactta gacccttatt ggcgatgtgg tttttgtttt gcctgtcttt cagacggcag
    51841 caaaaagcaa cttcccattt tttacgctga ttcagaattt agccataaaa aaacccgccg
    51901 aagcgggttt tttcgtaaat tgttttctgc cggagcagaa gccaattaac gtttggagaa
    51961 ctgcggacga cgacgtgctt tacgcagacc gactttctta cgttcaacct gacgagcgtc
    52021 acgagtaacg aagccagctt tacgcagttc agaacgcagg gactcgtcgt attccatcag
    52081 agcgcgggtg ataccgtgac ggatcgcacc agcctgacca gagataccac cacctttaac
    52141 ggtgatgtac aggtccagtt tctcaaccat gtcgaccagt tccagcggct gacgaactac
    52201 catgcgggca gtttcacgac cgaagtactg ttccagagaa cgttggttga ttacgatttt
    52261 accgttgccc ggtttgatga aaacgcgagc tgcggaattt ttgcggcgac cagtgccgta
    52321 gtattgattt tcagccattg cctataatcc cgattagatg tcaagaactt gcggttgctg
    52381 tgccgcgtgg ttgtgctcgt tacccgcgta aactttcagt ttacggaaca tagcacgacc
    52441 cagcgggcct tttggcaaca tgcctttaac cgcgatttca atcacacgct caggacggcg
    52501 agcaatcatc tcttcaaagc tcacttgttt gataccaccg atgtggccgg tgtggtgata
    52561 gtacactttg tcagtacgct tgttgccggt tacagcaact ttgtcagcgt tcagaacgat
    52621 gatgtaatca ccggtatcta cgtgcggagt gtattccgct ttgtgcttac cgcgtaggcg
    52681 acgagccagt tcagtagcca gacggcccag agttttaccg gtcgcgtcaa caacatacca
    52741 gtcgcgtttt acggtttctg gtttagctgt aaaagttttc attaaaagct tacccaataa
    52801 atagttacac gttggtgaac acccaaacgt cttcaattgt tgaggttcac acgacaaagt
    52861 ccggcaaacc taccccttcg aatagcctat gccagcacac aaaaagtttt gggaaaaaaa
    52921 ctttcttgta acgtggggtc gcaggattat agagaagtcg gggtcaaaga tcgacccctt
    52981 tttgtgattt gtgacaggtt ttaacccgcc aaatgctcgc gcttcagata ctcttcgctt
    53041 tgcatctctt gcagacgtga caggcaacgc tggaactcaa acttcagccg atcgccctga
    53101 taaatttcat acagcggcac ttctgcactc accactaatt tgacatggcg ctcgtaaaac
    53161 tcatccacca gcgcaataaa gcgccgcgct tcgctctcca tcaaccgcgt cataactggt
    53221 acatcaaaca acatgaccgt atgaaagaga cgtgagagcg caatatagtc atgctgactg
    53281 cgggcgtcga cgcacagcgt agtaaaagag accgccagcg tctggttctc gacgcccatt
    53341 gtcgctaatg gccgatggtt gatttctaac gtcggtgaat tttctcgttt cccccccgcc
    53401 agcgccaacc atagtttatc catttgcgcc cgggtttcat cgtggagtgg cgaaagccac
    53461 agatgcgcct gagtgagtgt acgcagtcga taatcaacac cagcgtccac gttcattaca
    53521 tcacaatgct gtttaatggc atcgattgca ggcagaaaac gcgcacgttg caagccattt
    53581 cgataaagtt cgtccggcgg aatatttgac gtcgctacca gggtaatacc gcgggcgaac
    53641 aaggctttca tcagaccgcc aagtagcatg gcatcggtaa tatcagaaac aaaaaattcg
    53701 tcaaaacaga gcacgtcagt ttcggcttta aagcgatcgg caataatttc cagcggatcg
    53761 gtctgcccct gtaaggcagt tagctcttca tgcacccgca gcataaaacg gtgaaagtgc
    53821 aggcgctgtt tccgctctcc cggcaggctt tgatagaaaa ggtccatcag ccaggttttc
    53881 ccgcgtccta caccgcccca catatataag ccacgcactg gcgtatgctt tgtgtcttcg
    53941 cgtttacccc acagcttacc gacccgcgcc attagcccac tcgtcctggg ggctggtggc
    54001 gtgctattga tgagttcctg ataaataatt tccaggcggc tgacggcctc tttttgaacg
    54061 tcgtcgggtt gatggctgcc ttcattgagc gccttcaggt attgcgatgt tggggtaacg
    54121 ctttgcatga tcttattgtt attccttgaa taatcggtgc gccgttgttc acggttgacg
    54181 aaaaaaaggc cgttctacac tacgcgatat gcagtcggga ttccacttct gtggaattaa
    54241 cggttatagt ggcataatca gccgcaggca tggagcctga agccaacacc ctacggaaac
    54301 aaaagacaac gggagatgtt catgacctgg gaatatgcgc taattgggtt agtcgtcggc
    54361 atcattattg gtgctgtggc catgcgtttt ggtaatcgta aactacgcca gcaacaggcg
    54421 ttgcagtacg aactggaaaa gaataaagct gaactggacg agtatcgcga agagctggtt
    54481 agccactttg cccgcagcgc ggaattactg gataccatgg cgcacgacta tcgccagctg
    54541 tatcagcaca tggcgaaaag ttccagcagc ctgctgccgg aactgtctgc tgaagcaaac
    54601 ccgttccgta atcgtctggc agagtctgaa gccagcaacg atcaggcacc ggtgcagatg
    54661 cctcgcgact attctgaagg cgcatccggc ctgctgcgta ctggcgcgaa gcgcgactaa
    54721 tttatttttc gggcgcagcc attgcgccct cctcttctct ccctccccga ctatcattta
    54781 atctggtgtc tcattgttag ccgtctgaaa attcaataac atcaaactgt tttgaatctc
    54841 ttttcttatc attcaggtac gagagcagga ataatgaaaa aacaacccca gctgttgagt
    54901 gcattagcgt taagtgtcgg gttaactctc tcggcgtcat ttcaggccgt cgcgtcgatt
    54961 ccaggccagg ttgccgatca ggcccctctc cccagtctgg ctccaatgct ggaaaaagtg
    55021 cttccggcag tggtgagcgt acgggtggaa ggaacggcca gtcagggaca gaaaatcccg
    55081 gaagaattca aaaagttttt tggtgatgat ttaccggagc aacctgcaca acccttcgaa
    55141 ggtttaggct ccggtgtcat catcaacgcc agtaaaggct atgtgctgac caacaaccat
    55201 gtgattaatc aggcacagaa aatcagtatt cagctcaatg atgggcgcga gtttgatgca
    55261 aaactgattg gtagcgatga ccagagcgat atcgccctgt tacaaattca aaacccgagc
    55321 aaattaacgc aaatcgctat tgccgactcc gataaattgc gcgtcggtga ttttgccgta
    55381 gcggtcggta acccatttgg ccttgggcaa accgccacct ctggcattgt ttccgcatta
    55441 ggacgcagcg ggttgaatct tgaaggtctg gaaaacttta tccagacaga tgcttccatt
    55501 aaccgcggta actccggcgg tgcactgtta aaccttaacg gtgagttaat tggcatcaac
    55561 actgcaatcc ttgcgcctgg cggcgggagc gtcgggattg gatttgccat ccccagtaat
    55621 atggcgcgaa cactggcgca gcagcttatc gactttggtg aaatcaaacg cggtttgtta
    55681 ggcatcaaag gcaccgagat gagtgccgat atcgccaaag ccttcaacct tgacgtgcag
    55741 cgtggcgcgt ttgtcagcga agtgttgcca ggttctggct cggcgaaagc gggcgtcaaa
    55801 gcgggcgata ttattaccag cctcaacggc aaaccgctga atagctttgc tgagttgcgc
    55861 tctcgtatcg cgaccaccga gccgggcaca aaagtgaaac ttggcctgct gcgtaacggc
    55921 aaaccactgg aagtagaagt gacgctcgat accagcacct cttcgtcggc cagcgctgaa
    55981 atgatcgcgc cagcgctgga aggtgcaacg ttgagcgatg gtcagctaaa agatggcggc
    56041 aaaggtatta agatcgatga agttgtcaaa ggaagcccag ctgctcaggc tggcttgcaa
    56101 aaagacgatg tgatcattgg cgtcaaccgc gatcgggtga actcgattgc tgaaatgcgt
    56161 aaagtgctgg cggcaaaatc ggccatcatc gccctgcaaa ttgtacgcgg caatgaaagc
    56221 atctatctgc tgatgcgtta atgtcgtaaa ccgggcatca ggcttacgtg tgatgtccgg
    56281 ttaactcgtg gtatgctgct gccgttccct tttttaatga cgcctccatc atgtttgtga
    56341 agctcttacg ttccgttgcg attggattaa ttgtcggcgc tattctgctg gttgccatgc
    56401 cttcgctgcg cagccttaac ccgctttcca ctccgcaatt tgacagtacc gatgagacgc
    56461 ctgccagcta taatctggcg gttcgccgcg ccgcgccagc ggtggttaac gtttacaacc
    56521 gtggtttgaa caccaactct cacaaccagc ttgagatccg caccctggga tccggtgtaa
    56581 tcatggatca acgcggttat atcatcacca ataaacacgt catcaacgac gccgatcaga
    56641 tcatcgtcgc cttacaggat ggtcgtgtat ttgaagcatt gctggtggga tctgactctc
    56701 taaccgatct ggcggtactt aaaattaatg ccactggcgg tttacctacc attccaatca
    56761 atgcacgtcg cgtaccgcat attggcgacg tggtactggc gatcggtaac ccgtacaacc
    56821 tcgggcagac cattacccag gggattatta gtgccacggg tcgaattggt ctgaacccga
    56881 ccgggcggca aaacttcctg caaaccgatg cttccattaa ccacggtaac tctggcggcg
    56941 cgctggtgaa ctcgctgggc gaactgatgg gcattaacac gctgtcgttt gataagagta
    57001 acgatggcga aacgccggaa ggtatcggct ttgcgattcc tttccagtta gcaaccaaaa
    57061 ttatggataa gctgatccgc gatggtcgcg tgatccgcgg ctacattggc attggcggac
    57121 gtgagatcgc accgctgcac gcacagggcg gtggtataga tcaactgcaa gggatcgtgg
    57181 ttaatgaagt gtcacctgac ggcccggcgg cgaatgcggg tattcaggtc aacgatctga
    57241 ttatttcggt ggataacaaa ccggccatct ctgctctgga gacgatggat caggtggcgg
    57301 aaattcgccc tggttcggtg atcccggttg tagtgatgcg tgatgataag cagttaacgc
    57361 tgcaggtcac cattcaggaa tatccggcaa ccaattaagt cgagtgctca aaacaaaaaa
    57421 ccggagtctg tg
//
LOCUS       DAEEKQ010000010        46667 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.010, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000010 DAEEKQ010000000
VERSION     DAEEKQ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 46667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 46667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..46667
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.010"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..135)
                     /gene="tabA"
                     /locus_tag="KLK57_000618"
     CDS             complement(<1..135)
                     /gene="tabA"
                     /locus_tag="KLK57_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBE0891084.1"
                     /translation="MIIGNIHNLQPWLPQELRLAIEHIKAHVTAETPKGKHDIEGNRL
                     F"
     gene            complement(280..873)
                     /locus_tag="KLK57_000619"
     CDS             complement(280..873)
                     /locus_tag="KLK57_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE0891085.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTILKE"
     gene            944..1657
                     /gene="bdcA"
                     /locus_tag="KLK57_000620"
     CDS             944..1657
                     /gene="bdcA"
                     /locus_tag="KLK57_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0891086.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     EHLAQKTGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMAREL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            1788..2183
                     /locus_tag="KLK57_000621"
     CDS             1788..2183
                     /locus_tag="KLK57_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBE0891087.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPENQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            2464..2598
                     /gene="pyrL"
                     /locus_tag="KLK57_000622"
     CDS             2464..2598
                     /gene="pyrL"
                     /locus_tag="KLK57_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HBE0891088.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            2602..3537
                     /gene="pyrB"
                     /locus_tag="KLK57_000623"
     CDS             2602..3537
                     /gene="pyrB"
                     /locus_tag="KLK57_000623"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HBE0891089.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            3550..4011
                     /gene="pyrI"
                     /locus_tag="KLK57_000624"
     CDS             3550..4011
                     /gene="pyrI"
                     /locus_tag="KLK57_000624"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HBE0891090.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            4084..4470
                     /gene="ridA"
                     /locus_tag="KLK57_000625"
     CDS             4084..4470
                     /gene="ridA"
                     /locus_tag="KLK57_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HBE0891091.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="KLK57_000626"
     CDS             complement(4676..7372)
                     /gene="mgtA"
                     /locus_tag="KLK57_000626"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HBE0891092.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="KLK57_000627"
     CDS             complement(7513..7566)
                     /gene="mgtL"
                     /locus_tag="KLK57_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HBE0891093.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            7751..8698
                     /gene="treR"
                     /locus_tag="KLK57_000628"
     CDS             7751..8698
                     /gene="treR"
                     /locus_tag="KLK57_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HBE0891094.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            8817..10238
                     /gene="treB"
                     /locus_tag="KLK57_000629"
     CDS             8817..10238
                     /gene="treB"
                     /locus_tag="KLK57_000629"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HBE0891095.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            10288..11943
                     /gene="treC"
                     /locus_tag="KLK57_000630"
     CDS             10288..11943
                     /gene="treC"
                     /locus_tag="KLK57_000630"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HBE0891096.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVQHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            12337..14475
                     /gene="nrdD"
                     /locus_tag="KLK57_000631"
     CDS             12337..14475
                     /gene="nrdD"
                     /locus_tag="KLK57_000631"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HBE0891097.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSRESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            14633..15097
                     /gene="nrdG"
                     /locus_tag="KLK57_000632"
     CDS             14633..15097
                     /gene="nrdG"
                     /locus_tag="KLK57_000632"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HBE0891098.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="KLK57_000633"
     CDS             complement(15142..15528)
                     /gene="cybC"
                     /locus_tag="KLK57_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HBE0891099.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVVEKADN
                     ATQVKDALTKMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="KLK57_000634"
     CDS             complement(15712..17064)
                     /gene="pmbA"
                     /locus_tag="KLK57_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HBE0891100.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGLADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLLPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            17158..17709
                     /locus_tag="KLK57_000635"
     CDS             17158..17709
                     /locus_tag="KLK57_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HBE0891101.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="KLK57_000636"
     CDS             complement(17792..19165)
                     /gene="mpl"
                     /locus_tag="KLK57_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HBE0891102.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVASADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            19341..20339
                     /gene="fbp"
                     /locus_tag="KLK57_000637"
     CDS             19341..20339
                     /gene="fbp"
                     /locus_tag="KLK57_000637"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HBE0891103.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENAQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEY
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="KLK57_000638"
     CDS             complement(20372..21367)
                     /gene="yjfF"
                     /locus_tag="KLK57_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HBE0891104.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQQ"
     gene            complement(21354..22376)
                     /locus_tag="KLK57_000639"
     CDS             complement(21354..22376)
                     /locus_tag="KLK57_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0891105.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSGVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSRDKT"
     gene            complement(22390..23892)
                     /locus_tag="KLK57_000640"
     CDS             complement(22390..23892)
                     /locus_tag="KLK57_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBE0891106.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFFLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHVLQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="KLK57_000641"
     CDS             complement(24032..24988)
                     /gene="ytfQ"
                     /locus_tag="KLK57_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HBE0891107.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTGGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMMDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            25298..25828
                     /gene="ppa"
                     /locus_tag="KLK57_000642"
     CDS             25298..25828
                     /gene="ppa"
                     /locus_tag="KLK57_000642"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HBE0891108.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(26207..26548)
                     /locus_tag="KLK57_000643"
     CDS             complement(26207..26548)
                     /locus_tag="KLK57_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HBE0891109.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="KLK57_000644"
     CDS             complement(26551..30330)
                     /gene="tamB"
                     /locus_tag="KLK57_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HBE0891110.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGNVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDIILNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="KLK57_000645"
     CDS             complement(30327..32060)
                     /gene="tamA"
                     /locus_tag="KLK57_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HBE0891111.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQILDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSDWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGGGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            32266..32904
                     /gene="msrA"
                     /locus_tag="KLK57_000646"
     CDS             32266..32904
                     /gene="msrA"
                     /locus_tag="KLK57_000646"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HBE0891112.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            33227..34570
                     /locus_tag="KLK57_000647"
     CDS             33227..34570
                     /locus_tag="KLK57_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBE0891113.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(34633..34839)
                     /locus_tag="KLK57_000648"
     CDS             complement(34633..34839)
                     /locus_tag="KLK57_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HBE0891114.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            35164..35718
                     /locus_tag="KLK57_000649"
     CDS             35164..35718
                     /locus_tag="KLK57_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HBE0891115.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLQKLLK"
     gene            complement(35808..36719)
                     /locus_tag="KLK57_000650"
     CDS             complement(35808..36719)
                     /locus_tag="KLK57_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891116.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSYTKTHSNNFFNNIQHAFKKKDWI
                     SNYDSLLTLREFFRCATQIDKNSYQVLSSKNGTVNAMDKFLISFSLKDNGAEYTMTLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIHQ
                     AIGDVSLGYALLEDIVSKLKRGKFELQIPGGGIKECDGWYIHEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICKKYKSTIEAFKKEQLALPV"
     gene            complement(36931..37671)
                     /gene="cysQ"
                     /locus_tag="KLK57_000651"
     CDS             complement(36931..37671)
                     /gene="cysQ"
                     /locus_tag="KLK57_000651"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HBE0891117.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            37861..39804
                     /gene="cpdB"
                     /locus_tag="KLK57_000652"
     CDS             37861..39804
                     /gene="cpdB"
                     /locus_tag="KLK57_000652"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HBE0891118.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKTGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VVVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINTNAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(39922..40302)
                     /locus_tag="KLK57_000653"
     CDS             complement(39922..40302)
                     /locus_tag="KLK57_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HBE0891119.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            40391..41251
                     /gene="qorB"
                     /locus_tag="KLK57_000654"
     CDS             40391..41251
                     /gene="qorB"
                     /locus_tag="KLK57_000654"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBE0891120.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            41359..42324
                     /locus_tag="KLK57_000655"
     CDS             41359..42324
                     /locus_tag="KLK57_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBE0891121.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALVCIGIGLACVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            42432..43094
                     /gene="ytfE"
                     /locus_tag="KLK57_000656"
     CDS             42432..43094
                     /gene="ytfE"
                     /locus_tag="KLK57_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HBE0891122.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(43139..44551)
                     /gene="cycA"
                     /locus_tag="KLK57_000657"
                     /gene_synonym="dagA"
     CDS             complement(43139..44551)
                     /gene="cycA"
                     /locus_tag="KLK57_000657"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HBE0891123.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(44860..45480)
                     /gene="fklB"
                     /locus_tag="KLK57_000658"
     CDS             complement(44860..45480)
                     /gene="fklB"
                     /locus_tag="KLK57_000658"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE0891124.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            45699..46337
                     /gene="ytfB"
                     /locus_tag="KLK57_000659"
     CDS             45699..46337
                     /gene="ytfB"
                     /locus_tag="KLK57_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HBE0891125.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            complement(46321..>46667)
                     /locus_tag="KLK57_000660"
     CDS             complement(46321..>46667)
                     /locus_tag="KLK57_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE0891126.1"
                     /translation="PWFLKIVHSENQSKGVHYAKSLRLLEINHAHLQLMESLLDEGKK
                     HNIFKPDIDPLQVNINIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILS
                     WLLVDPSSTAHE"
BASE COUNT        11452 a        12411 c        11630 g        11174 t
ORIGIN      
        1 aaacagacga ttgccttcga tatcgtgctt gccctttggc gtttctgccg taacgtgtgc
       61 tttgatatgc tcaatcgcca ggcgtaactc ctggggtagc cacggctgaa gattatgaat
      121 atttccgatg atcatctttt ccactcctta attaaaacaa tgtttcattt ccatcgttat
      181 acgaaaagat gatcaattcc gctagcctta atgcgtgaat tattgtctta tcacaactta
      241 tcggcaggcg cgttcccgcc tttcgggtca aggcccgcat cactccttga gaatagtttt
      301 tatcgcttcc cccgccagtg cagctgttgc acagagttgt tcgatggagt gcccctcccg
      361 agccttcgca gaaagccctg acatcacggt actcataaaa tcagtcacac attgtgcgct
      421 ttgtggatgc cgcctggcga tatagtcata aatggccgtt tctgcggcgt gataatattg
      481 aacggcaata tcacgcgctt gtggatcatg actatgaatg ccttcaagaa ccatacagcc
      541 agcgcagccg ccgttttggc tatatctgcg cgccgcttct tttaatacct cagccaggca
      601 ctcccctacc ggacgatcat cacgaagaat atcggcaagc ggaatagctt ccgtaccgac
      661 atattcattg agtacacggc taaataaccc agccttactg ccaaaagccg cgtagaggct
      721 cggggggtta ataccaagat aatcagtaac ttcagcaaca ctgacagcat cgaaaccttt
      781 ttggtgaaaa agcacttttg ccgcagagac tgcctgctca ggagcgaaac gtctgggacg
      841 acctggaacg cgagattgtt ttttagtgac cattacaaaa cttgttgaca gaaagttaaa
      901 acagttttgt aatgcatgtt acataataaa tcaaggagtc cttatgggcg cttttacagg
      961 taagacagtt ctcatcctcg gtggcagtcg tggtatcggt gccgctatcg tacgtcgttt
     1021 cgtcaccgat ggggccaatg tacgattcac ctatgcgggg tcgaaagatg ccgctgaaca
     1081 cctggcacaa aagactggag cgacagcagt attcacagat agtgctgaca gagacgctgt
     1141 cattgatgtc gttcgtaaga gcggcgcatt ggatatcctg gtggtaaatg caggtattgg
     1201 cgtctttggc gatgccctgg aattgaatgc cgacgatatt gatcgccttt tcaaaatcaa
     1261 tattcatgct ccttatcatg cctctgttga agccgcccgg cagatgcccg aaggcgggcg
     1321 catcttaatc atcggctccg tgaatggcga tcgtatgcct gttgcaggca tggctgctta
     1381 tgccgccagt aaatctgccc tgcaaggcat ggcgcgcgag ctggcccgtg atttcggacc
     1441 gcgtgggatc accatcaacg tcgtccagcc agggccaatt gataccgacg ctaatcccgc
     1501 caacgggcca atgcgcgata tgttgcatag ttttatggct atcaaaagac atgggcaacc
     1561 ggaagaggtc gctggtatgg tcgcatggtt agcagggcca gaagccagtt ttgttaccgg
     1621 cgcgatgcat accattgatg gcgcgtttgg cgcataaccg actacgctca attaagccca
     1681 gccatttccc atgatgtctg ggttttgttt actcacgtcg tccgctaaaa gcggctcctg
     1741 gtaaatataa atcttctgtt catgtcgtaa atcttgagga taaccaaatg gtagaaagaa
     1801 ccgctgtttt ccctgcaggc cgacattcac tgtatgctga acatcgttat tctgcggcta
     1861 ttcgttccgg cgatttgctg tttgtttccg ggcaagtagg aagtcgagag gacggaacac
     1921 cagaacccga ttttcagcaa caagtcagac tggcatttga taatttgcat gcgaccctgg
     1981 ctgctgcggg atgcactttt gacgatatca ttgatgttac gagcttccat accgatccag
     2041 aaaaccaatt tgaagacatc atgacggtga aaaatgaaat atttagcgcc ccaccttatc
     2101 caaactggac ggcggtaggt gttacatggc tggcaggctt tgattttgaa attaaagtga
     2161 tagcgcgcat ccctgagcag taagcaatag tgttagccgt tcgttttcac actccgccct
     2221 ataagtcgga tgaatggaat aaaatgcata tctgattgcg tgaaagtgaa aaaggaaaaa
     2281 gcaggaaatg tctgcaatta ttgataccga acgaccgttc cccagcagaa tcacaacaaa
     2341 taaaaatgca tgtattttga cttataattc aaataaaccg tttgcgctga caaaatattg
     2401 catcaaatgc ttgcaccgct tctgacgatg agtataatgc cggacaattt gccgggagga
     2461 tgtatggttc agtgtgttcg acattttgtc ttaccgcgtc tgaaaaaaga cgctggcctg
     2521 ccgtttttct tcccgttgat cacccattcc cagcccctca atcgaggggc ttttttttgc
     2581 ccaggcgtca ggagataaaa gatggctaat ccgctatatc agaaacatat catttccata
     2641 aacgacctta gtcgcgatga ccttaatctg gtgctggcga cagcggcgaa actgaaagca
     2701 aacccgcaac cagagctgtt gaagcacaaa gtcattgcca gctgtttctt cgaagcctct
     2761 acccgtaccc gcctctcttt cgaaacttcc atgcaccgcc tgggtgccag cgtggtgggc
     2821 ttctccgaca gcgccaatac atcactgggt aaaaagggcg aaacgctggc cgataccatt
     2881 tcggttatca gcacttacgt cgatgcgata gtgatgcgtc atccgcagga aggtgcggcg
     2941 cgcctggcca ccgagttttc cggcaatgta ccggtactga atgccggtga tggctccaac
     3001 caacatccga cgcaaacctt gctggactta ttcactattc aggaaaccca ggggcgtctg
     3061 gacaatctcc acgtcgcaat ggttggtgac ctgaaatatg gccgcaccgt tcactccctg
     3121 actcaggcgt tagcgaagtt cgacggcaac cgtttttact tcatcgcgcc ggacgcgctg
     3181 gcaatgccgc aatacattct ggatatgctt gatgaaaaag ggatcgcctg gagtctgcac
     3241 agctctattg aagaagtgat ggcggaagta gacatcctgt acatgacccg cgtgcaaaaa
     3301 gagcgtctgg acccgtccga gtacgccaac gtgaaagcgc agtttgttct tcgcgccagc
     3361 gatctccaca acgccaaagc caatatgaaa gtgctgcatc cgctgccgcg tgttgatgag
     3421 attgcgacgg atgttgataa aacgccacac gcctggtact tccagcaggc aggcaacggg
     3481 attttcgctc gccaggcgtt actggcactg gttctgaatc gcgatctggt actgtaaggg
     3541 gaaatagaga tgacacacga taataaattg caggttgaag ctattaaacg cggcacggta
     3601 attgaccata tccccgccca gatcggtttt aagctgttga gtctgttcaa gctgaccgaa
     3661 acggatcagc gcatcaccat tggtctgaac ctgccttctg gcgagatggg ccgcaaagat
     3721 ctgatcaaaa tcgaaaatac ctttttgagt gaagatcagg tagatcaact ggcattgtat
     3781 gcgccgcaag ccacggttaa ccgtatcgac aactatgaag tggtgggtaa atcacgcccc
     3841 agtctgccgg agcgcatcga caatgtgctg gtctgcccga acagcaactg tatcagccat
     3901 gccgaaccgg tttcttccag ctttgccgtg cgaaaacgcg ccaatgacat cgcgctcaaa
     3961 tgcaaatact gtgaaaaaga gttttcccat aatgtggtgc tggccaatta attgcggttg
     4021 gtaataaaag tctggctccc tataatgagc cagacttttt accgctgtaa taaaggagaa
     4081 atcatgagca aaactatcgc gacggaaaat gcaccggcag ctatcggtcc ttacgtacag
     4141 ggcgttgatc tgggcaatat gatcatcacc tccggtcaga tcccggtaaa tccgaaaacg
     4201 ggcgaagtac cggcagacgt cgctgcacag gcacgtcagt cgctggataa cgtaaaagcg
     4261 atcgtcgaag ccgctggcct gaaagtgggc gacatcgtta aaaccaccgt gtttgtgaaa
     4321 gatctgaacg acttcgcaac cgttaatgct acctacgaag ccttcttcac cgaacacaat
     4381 gccaccttcc cggcacgttc ttgcgttgaa gttgcccgtc tgccgaaaga cgtgaaaatt
     4441 gagatcgaag cgatcgctgt tcgtcgctaa tcttgatgga aatccgggct atcatgcccg
     4501 gattaagtct gatgacaaac acaaaatcgc ctgatgcgct acgcttatca ggcctacgtg
     4561 attcctgcaa tttattgaat ttgttggccg gataaggcat ttacgccgca tccggcatga
     4621 acaaaactca ctttgtctgc aatctgaatc caggccacca tgcccagttg attctttatt
     4681 gccagccgta acgacggcta taaaatcctt tcaccagttg ggttaacgtc atataccctg
     4741 ccagaatcgc aaccagccac gggaaatagc ttaacggcag cgcctgcagt tgcagataac
     4801 tggccagcgg tgaaaacggc aatgcgatcc cgacaatcat cacgatcacg gtcatgatca
     4861 ttaacggcca cgatgcacag ctctgaataa acggcacacg gcgggtgcgg atcatatgca
     4921 caatcagcgt ttgcgacagc aagcccacca caaaccatcc cgactggaac agcgtttgcg
     4981 tttccggcgt gttggcatgg aatacccacc acatcaggca aaacgtcaga atatcgaaga
     5041 tcgagctgat cggtccgaag aagatcataa agcgccccag atccgccgga ttccaacgct
     5101 gcggcttttg aatttgctcg tcgtcgacgt tatcaaacgg gatcgccacc tgtgacacat
     5161 catacagcag gttctgaata agcaagtgta acggcaacat cggcaggaag ggcaagaaag
     5221 cactcgccac cagcacgctg aacacattac cgaagttgga gctcgccgtc attttgatgt
     5281 atttcagcat gttggcgaaa gtgcgacgtc cttcaataac cccctcttcc agcaccatca
     5341 ggcttttttc cagcaggatg atatcagccg cttcacgggc aatatctacc gcgccgtcca
     5401 cagaaatgcc gatatccgca gcgcgtaagg cgggcgcatc attaatacca tcgcccataa
     5461 agccaaccac atgcccttcg cgcttcagta aggtcacaat gcgttcttta tgcatcggcg
     5521 tcagacgagc aaacagcgtg gtgcgctgtg cgagatttgc cagttcgtcg tcagatagcg
     5581 tttcaatatc actaccaatg accacctctc ccgcgtccag ccccacttca tggcacactt
     5641 tcgctgcaac taactcactg tcgccagtaa ggatttttac ggtaattcca ctcgctttta
     5701 atgccttcag tgccggagca gttgtctctt ttggcggatc aaggaaagca atatatcctt
     5761 cgaggatcag gtcggattca tccgcccgct ggtaatctcc ttcacgcgct ggcaggtatt
     5821 tcgtcgccac cgcaaccacg cgcagcccct gacgattcag cgtatcagta acccgcttaa
     5881 tcttacgcag catgatgtca tcgagcggca caatctcgcc attgtgacgc acctgcgaac
     5941 acacattgag gatttcctgc aatgcacctt tgcaaaccag ctgatggtgc tcggtatttt
     6001 ctgccactac caccgacatc cggcgacgct cgaaatcaaa cggaatctca tcaatttttt
     6061 gccaacggct ggccagagag cgcgctgact cttcatccgt accttcgagc accgctgtat
     6121 cgagtaggtt tttaagtccg gtctgataat gactgttcaa ccacgcgcta tgcagcacgc
     6181 gttcgctggt tttaccggag atatcggtat gattctccag cacaattttg tcctgtgtca
     6241 gggtgccagt tttatcagtg cacagaatat ccattgcgcc aaagttctga atagcatcca
     6301 gatgtttgac gatcactttc tgtttcgaca gctttactgc gccgcgcgcc agcgtcgagg
     6361 taacaatcat cggcaacatt tccggcgtta agcctaccgc gaccgaaagc gcaaacagtg
     6421 ccgcttccca ccagtcgcct ttggtgtaac cgttgataag caacaccacc ggagccatca
     6481 ccagcataaa gcgaatcagc agcatactga cgcggctgat cccctgctga aaggcattcg
     6541 gctcgctttc ctgctcacta acacgccccg ccagttgacc aaaccaggtg ttggctcctg
     6601 tagcaatcac cattgcttgt gccgtgccgc tcaccacggt ggtgcccata aaacacaggg
     6661 tgtcacactc cagcggattg ctgtgctccg gctggcgagt ggtagcggct ttttccacgg
     6721 gcagagactc accggttaac gacgcctgag cgacgaacag atcccgcgcc tgcaagatac
     6781 gtaaatctgc cgggatcata tcccccgccg ccagttttat aatatcgccg ggcaccagct
     6841 ggtcgatcgg gatctccagc cagccatttt cgcctttgtc gttaattacg cgcagcaccg
     6901 tcgcggtatt gctgaccatc gctttcaggg catctgccgc tttagtggaa cgtgcttcct
     6961 gaataaagtt cagcaacgta gaaatagcga ccattagcgc gataacgccc gcggcaaata
     7021 aatcttcagt ggcgtaagaa atagcgccga gaatggtgag taaaatatta aatgggttgc
     7081 gatagcagac ccataaatgt acccaccacg gcgacggttg ttgtgcgggt aatttatttt
     7141 caccatgttg ttcgcgggca gattccactt ccgcctgatt taacccttcc ggatgggtgt
     7201 cgaacgtttt ccacaattct tcttcgggca tcaccgccat tttcaggcaa tgcgcactta
     7261 aggacggcgg gaccgccgta ttcactgtct gctgcgcgcc aggcaatgga tcgcgatgaa
     7321 ccagacggga aggtaaatgg cgaatgagcc gggtaaaaat ttctttaaac ataaggagtc
     7381 cctccgcact gtctgaaaca gtacagaaat gtcttcaggc gtggcgaagc cttacctgaa
     7441 ggtaataaag ctgagcaggg acgttaaaac gtcacggcct tacgcatccg gtagcgccgt
     7501 gaaaagacgg gcttaccgga aaagttttaa tctccgtcga gggagaggcg tgggatcagg
     7561 ttccatataa cctccggtaa gtgaaaaaag cgccgcaaat aataaggaga atccaataat
     7621 attcgtagca tttatggcga tataatactt gctggcaact aaaccaacga taaaccagac
     7681 tttacccatt gctgaatgca cgggtaaagt taggctcaaa taattaaaca acacgttaca
     7741 ggacaacagg atgcaaaatc ggctgactat caaagacatc gcacgcttaa gcggcgtggg
     7801 gaaatctaca gtttcccggg tgctgaataa cgaaagcggc gtgagccagc gcacccgcga
     7861 gcgtgttgaa gcagtgatga atcagcatgg attttcccct tcccgctctg cgcgcgctat
     7921 gcgtgggcaa agcgataaag tggtcgccat cattgttacc cgtctggatt cgttgtcaga
     7981 aaatctcgcc gttcaaacca tgctgccagc gttctatgaa caaggttacg acccaatcat
     8041 gatggaaagc cagttttccc cgcaattagt tgccgaacat ttgggggtgc tgaaacggcg
     8101 taatatcgac ggcgtagtgc tgttcggttt tactggcata acagaagaaa tgttagccca
     8161 ctggcagtca tcgctggttc tgctggcgcg tgacgcaaaa ggctttgctt cggtctgtta
     8221 tgacgacgaa ggggcaatca agatcctgat gcaacggctg tatgaccagg ggcatcgtaa
     8281 tatcagttat ctcggcgtgc cgcatagtga cgtgacaacc ggtaagcgac gtcacgaagc
     8341 ctacctggcg ttctgcaaag cgcataaact gcatcccgtt gccgctctgc cagggcttgc
     8401 tatgaagcaa ggctatgaga acgtggcaaa agtgattacg cctgaaacta ctgcgttact
     8461 gtgcgcaacc gacacgctgg cacttggcgc aagtaaatac ctgcaagagc aacgcatcga
     8521 caccttgcaa ctggcgagcg tcggtaatac accgttaatg aaattcctcc atccggagat
     8581 cgtcaccgta gatcccggtt acgccgaagc tggacgccag gcggcctgcc aattgatcgc
     8641 acaggtaacc gggcgcagcg aaccgcaaca aatcatcatc cccgccaccc tgtcctgatc
     8701 gtttcctgaa cgataaattg tgatcttcgc tgcatttcgg gaacgttccc gtttttaaat
     8761 ttttccgcgc aatatattct gcagccaacc aaaaatgtca tctgccatgg ggctttatga
     8821 tgagcaaaat aaaccaaacg gatatcgatc ggttgattga actggtcggc gggcgcggca
     8881 atattgcgac ggtgagccac tgtattactc gcctgcgctt tgtcctcaac caaccggcca
     8941 atgccagacc gaaagaaatt gagcaactcc ccatggtgaa aggctgtttc accaatgccg
     9001 ggcaatttca ggtggtgatt ggcaccaacg tgggtgatta ctatcaagca ctaatagcgt
     9061 caaccggaca ggcgcaggtt gataaagagc aggtaaaaaa agccgcccgg cagaatatga
     9121 aatggcatga gcagttgatc tctcatttcg cggagatctt cttcccgttg ctgcccgcgt
     9181 tgattagcgg cggtttgatc ctcggttttc gcaatgtgat cggcgatttg cccatgagca
     9241 acggtcagac gctggcgcaa atgtaccctt ccctgcaaac gatctacgat tttctgtggt
     9301 tgatcggtga agcgattttc ttctacctgc cggtcgggat ttgctggtca gcggtgaaaa
     9361 aaatgggcgg cacgccgatc cttggtatcg tgcttggcgt gacactggtt tccccccagc
     9421 tgatgaacgc ttatctgctc gggcagcagc tgccggaagt gtgggacttt ggcatgttca
     9481 gcatcgccaa agtgggctat caggcgcagg tgatcccggc actgttagcc gggctggcgc
     9541 tgggcgttat tgaaactcgc cttaaacgca tcgtaccgga ttacctctat ctggtggtgg
     9601 tgcccgtctg ttcgctgatc ctcgcggtgt tcctcgccca tgcgctgatt ggtccgtttg
     9661 gtcgcatgat tggcgatggc gttgcctttg cggtacgtca cctgatgacc ggcagctttg
     9721 ctccgattgg tgcggcattg tttggcttcc tgtacgcgcc gctggtgatc accggcgtac
     9781 accagaccac ccttgctatt gatttgcaga tgattcaaag catgggtggc acgccagtgt
     9841 ggccgctgat tgcgctgtcg aatatcgctc agggctccgc cgtgataggc attatcattt
     9901 ccagccgcaa gcacaatgaa cgcgagatct ccgtgcctgc cgctatctcc gcctggcttg
     9961 gggtcactga gcctgcaatg tacggcatca acctgaaata tcgcttcccg atgctgtgcg
    10021 cgatgattgg ttctggtctg gcaggattgc tatgcggcct gaacggcgtt atggcgaatg
    10081 gtatcggcgt agggggcctg ccgggaattc tctcgattca accgagctac tggcaggtat
    10141 ttgcgctggc aatggctatc gccatcatca tcccgattgt actcacctcg tttatctatc
    10201 agcggaaata ccgcttgggc acgctggaca ttgtttaatt ttcttcgggg cgcaattgcg
    10261 ccccctcgca tttgcaggaa taacgtaatg actaatcttc cccactggtg gcaaaacggc
    10321 gttatctacc agatttatcc aaagagtttt caggacacca cgggtagcgg caccggcgat
    10381 ttacgtggcg ttatccaacg cctggactat ctgcataaac tgggcgttga tgccatctgg
    10441 ctaaccccct tttatgtctc tccccaggtc gataacggtt acgatgtagc gaactatacg
    10501 gcgattgatc ccacctacgg cacgctggac gattttgacg aactggtgac gcaggcaaaa
    10561 tcgcgcggga ttcgtatcat tctcgatatg gtgtttaacc atacctctac ccaacatgcc
    10621 tggtttcgcg aggcgctgaa caaagaaagc ccttaccgcc agttttatat ctggcgcgat
    10681 ggcgaaccag aaacgccacc gaacaactgg cgttcaaaat ttggcggtag tgcgtggcgc
    10741 tggcatgcgg aaagcgaaca gtactatttg catctctttg caccagaaca ggcggatctc
    10801 aactgggaga atccagcggt acgcgcagag ctgaaaaaag tctgtgaatt ctgggccgat
    10861 cgtggggtcg acgggttgcg cctggatgtg gtgaatctga tctccaaaga cccgcgtttc
    10921 cctgaagacc tggatggcga cgggcgtcgc ttctacaccg acgggccacg agcacacgag
    10981 tttttgcacg agatgaaccg cgatgtgttt acgccacgcg ggttaatgac cgtaggtgaa
    11041 atgtcctcca ccagccttga gcattgccag cgatacgcgg cactgacagg cagtgaattg
    11101 tcgatgacct ttaattttca tcacctgaag gtcgattatc ccggtggtga aaaatggacg
    11161 ctggctaaac ctgactttgt ggcgttgaaa acattgttcc gccactggca acaaggaatg
    11221 cacaacgtgg catggaatgc cttgttctgg tgtaaccacg atcagccgcg cattgtttct
    11281 cgctttggtg atgaaggtga ataccgcgtg cctgcggcaa aaatgctggc gatggtgcag
    11341 catggcatgc agggaacgcc gtatatctac cagggcgaag agattggcat gaccaacccg
    11401 catttcacgc gcattactga ctatcgcgac gtggagagcc tcaatatgtt tgccgagctg
    11461 cgcaacgatg gtcgtgatgc cgacgagtta ttggcaatcc tcgccagtaa atcccgtgac
    11521 aacagccgca cgcccatgca atggagcaac ggcgataatg ccgggtttac ggctggcgaa
    11581 ccgtggattg gcctgggcga taactatcaa caaatcaacg tagaagccgc gctggccgat
    11641 gactcctcgg tgttttacac ctaccaaaag ttaatcgcac tgcgtaagca agaagccatc
    11701 ctgacatggg gcaattacca ggatctgctg ccaaacagcc ctgtattgtg gtgctatcgc
    11761 cgtgaatgga aggggcaaac cttgctggtc attgccaacc ttagccgtga gatccaaccc
    11821 tggcagccag ggcaaatgcg cggcaactgg cagcttgtga tgcataacta cgaagaagcc
    11881 tcaccacaac cctgtgccat gaatttacgg ccttttgagg ctgtctggtg gttacagaag
    11941 taaatcttcc ctaagccccg gtaatgccgg tcattccggg gttttgtctg tcagcatgtt
    12001 gtttttgttg atttttcaac cagcaaattc attaaaaaat ttacataacg ctgtagcgcc
    12061 cgtcatccgt acgctctgct ttttactttg agctacatca aaaaaagtta aaacatcctt
    12121 gatgcaaagc actatatata gactttaaaa tgcgtcccaa cccaatatgt tgtattaatc
    12181 gactataatt gctactacag ctccccacga aaaaggtgcg gcgttgtgga taagcggatg
    12241 gcgattgcgg aaagcaccgg aaaacgaaac gaaaaaaccg gaaaacgcct ttcccaattt
    12301 ctgtggataa cctgttctta aaaatatgga gcgatcatga caccgcatgt gatgaaacga
    12361 gacggctgca aagtgccgtt taaatcagag cgcatcaaag aagcgattct gcgtgcagct
    12421 aaagcagcgg aagtcgatga tgccgattat tgcgccactg ttgccgcggt tgtcagcgag
    12481 cagatgcagg gccgcaacca ggtggatatc aacgagatcc agaccgcagt tgaaaaccag
    12541 ctgatgtcgg gtccatacaa acaactggct cgtgcttaca tcgagtaccg tcacgatcgc
    12601 gacattgaac gtgaaaaacg cggtcgcctg aaccaggaga tccgtggtct ggtcgagcag
    12661 accaacgcct cgttactcaa cgaaaacgcc aacaaagaca gcaaggtgat tccaacccag
    12721 cgcgacctgc tggccgggat cgtggctaaa cactatgcac gccagcacct gctgccgcgt
    12781 gacgtggtgc aggcacatga gcgtggcgat attcactatc acgatctcga ttactcgccg
    12841 ttcttcccga tgttcaactg catgttgatc gacctgaaag gcatgctgac ccagggcttt
    12901 aaaatgggga acgccgagat tgaaccgccg aagtcgatct ctacggcaac cgcggtaact
    12961 gcgcagatta ttgctcaggt cgccagccat atttatggcg gcaccaccat taaccgtatc
    13021 gatgaagtgc tggcgccgtt tgttaccgcc agctacaaca aacatcgcaa gaccgcagaa
    13081 gagtggaaca tcccggacgc cgaaggctac gctaactctc gtaccatcaa agagtgctac
    13141 gatgccttcc agtcgctgga gtacgaagta aacaccctgc acaccgccaa cggtcagacg
    13201 ccgtttgtaa cctttggttt tggtctgggc accagcaggg aatcgcgcct gattcaggaa
    13261 tcgatcctgc gtaaccgtat cgcaggcctc ggtaaaaacc gtaaaactgc ggtgttcccg
    13321 aaactggtgt ttgcgattcg cgatggcctg aaccataaaa aaggcgatcc gaactacgac
    13381 atcaaacagc tggcgctgga gtgcgcaagc aagcgcatgt atccggatat cctgaactac
    13441 gatcaggtag tgaaagtcac cggttcgttt aaaaccccga tgggctgccg cagcttcctc
    13501 ggcgtgtggg aaaatgaaaa cggcgagcag atccacgatg gtcgtaacaa cctcggcgtg
    13561 atcagcctga acctgccgcg tattgctctg gaagcaaaag gcgatgaagc caccttctgg
    13621 aagctgctgg atgaacgtct ggtgctggca cgtaaggcac tgatgacccg tatcgctcgt
    13681 ctcgaaggcg tgaaagcgcg cgtcgccccg atcctctata tggaaggtgc ttgtggcgta
    13741 cgtctgaatg ctgacgatga tgtttctgaa atcttcaaaa acggtcgtgc gtctatttcg
    13801 ctgggttaca tcggcatcca cgaaaccatt aacgcgctgt tcggcggcga gcatgtttac
    13861 gacaacgagc agcttcgcgc gaaaggtatc gccattgttg aacgtctgcg tcaggcagtg
    13921 gatcagtgga aagaagaaac gggttatggt ttcagtctct acagcacgcc gagtgaaaac
    13981 ctgtgcgacc gcttctgccg tctcgatact gctgagtttg gcgtggtgcc gggcgtgacc
    14041 gataaaggtt actacaccaa cagtttccac cttgatgtgg agaagaaggt gaacccgtac
    14101 gacaagatcg actttgaagc gccttacccg ccgctggcga acggtggttt catttgctac
    14161 ggcgagtatc caaacattca gcacaacctg aaagcgctgg aagatgtctg ggattacagc
    14221 tatcagcatg taccgtatta cggcaccaat acaccgattg atgagtgcta cgagtgtggc
    14281 tttaccggtg agttcgagtg caccagcaaa ggcttcactt gcccgaaatg tggtaaccat
    14341 gacgcctccc gtgtgtcggt aactcgccgc gtgtgcggat atttaggtag cccggatgcg
    14401 cgtccgttta acgccggtaa gcaggaagaa gttaagcgcc gcgttaaaca tttggggaat
    14461 gggcagatag gttaatcttc actctgcgtt aagcaaatgc cggatgcggc gtaaacgcct
    14521 tatccggcct acatttggca acggctgtag gcctgataag acgcgacagc gtcgcatcag
    14581 gcattgatta ccggatgcag cctacacgcg gactgataag ccaggcgaaa taatgaatta
    14641 tcatcagtac tatcctgtcg acatcgtcaa cggccccggc actcgctgca ccctgtttgt
    14701 ctccgggtgt gttcatgaat gccccggttg ctataacaaa agcacatggc gggtaaattc
    14761 tggtcagcca tttaccaaag caatggaaga caagatcatt aaagatctga atgacactcg
    14821 tatcaaacgc caggggattt ccctctccgg cggcgatccg ctgcatccgc aaaacgtgcc
    14881 ggatattctg aaactggtaa aacgcatccg cgccgagtgt ccgggtaaag acatctgggt
    14941 gtggacaggc tataaactcg acgaactcaa cgctgcgcaa atgcaggttg ttgatttgat
    15001 taacgtgctg gtcgacggca aatttgtgca ggatttaaaa gacccttccc tgatctggcg
    15061 cggcagcagc aatcaggtgg tgcatcattt gcgatgataa gttccctgtt acctgagtgc
    15121 aggcaacagg gaaatgagga attaacgata cttctggtga taggcgttgc gggtcgtttt
    15181 cagttgctct gcagcagcct gcgcttcttt tactttacct tcatttgcca gcttcagcgc
    15241 gtcgtcaatc tgaccgacca gaatgtcgaa accgtggcgg aaatctttca tttccgggct
    15301 gtccggtgat ttatcttcga gcttcggcgg cgttgctttt tgcgcatcca gcgctgcggc
    15361 gcgcatcttc gttaacgcgt ctttgacttg cgtcgcgtta tcggcttttt cgaccacttt
    15421 taaattgtcg ttgagggttt ccatattgtc ttcaagatca gcggcaaacg acgccgaact
    15481 gaataccaac gaggagactg caagaatagc taacaggctt ttacgcattg ctcacttcct
    15541 tttttattta cacttcatcc ttcacactgc ctcatcgaca gctacgctca ttcgaccggg
    15601 tcacgtacta cgagtacgct ccccggctct cattcacttt ccgtctcgat gcagtgcgaa
    15661 tgattttgtg taatttttta gtttatagcg cggcaggtcg cgccagtttt tttactgtcc
    15721 ggcgattttc atctccggca acagcacaga accacactgt atattactgc gtgtttcaat
    15781 atcgttaccg acggtgacaa tattgcgcca catatctttt aaattacctg cgatggtgat
    15841 ttcgctcacc ggatactgaa tttcgccatt ctctacccag aagcccgcag caccacggga
    15901 ataatcaccg gtaatggcac tcacgccctg gcccatcagc tccgtcacca ccagcccggt
    15961 gcccatctct ttgagcattt gttcgaagct tagcccttgt ccggcaatcc gccagttgtg
    16021 aataccgccc gcatgtccgg tgcttttcag cccaagtttt cgcgccgaat agctggtcag
    16081 cagccactga gtcaggatgc catctttaat aatatcgcga cgctcggtgc gcacaccttc
    16141 gctgtcgaat ggcgtcgacg ccagcccttt cagcagatgc ggatgctctt caatggtcag
    16201 ccagtccggc agaatttgtt tacccagcga atccagcagg aaggtagatt tacgataaac
    16261 cgatccaccc gctatcgccc ccaccagatg cccaaaaagc ccagttgcca cttcattggc
    16321 aaaaatgact ggcgctttca tggtggagag tttacgcggt aacaggcgcg ataaagtacg
    16381 gcgagcacag tcggccccaa cccactctgg cgtttgcaga tcgctcatcg cgcgcccaat
    16441 ggtgtaggcg taatcacgct ccatatcgcc attttcttcg gcaattacac agctggagag
    16501 cgaatgacgc gttgagcagt aaccctgcaa catgccgtgg ctgttgccaa aaactttgac
    16561 accgtagtgg ctgttaaagc tgccaccttc ggtattggtg atacgtttat ccgcctgtaa
    16621 tgctgcctgt tctgcgcggg ccgccagttc aatggcttca tccggggaaa cttccgcagg
    16681 gtggaacaag tcgagatctg gtgcgtcaaa ggccagcagc tctttgtctg ccaggccggc
    16741 acagggatct ggcgaggtat aacgggcaat atccagcgcc gcctgtacag tacgggcaat
    16801 ggcctgcggg cttaaatcgg tggatgatgc gctacctttg cggttctggt gataaacagt
    16861 gatccccagc gcgccatcgc tattgaattc aacattctcc acttcaccat aacgcgtgct
    16921 tacgctaatg ccggtggtct tgctgacggc aacttccgca ccgtccgatt tgcctgaggc
    16981 caactccagc gcagtggaaa ctgcttcttc cagaatcttg cgctgcgctt caacttgaga
    17041 gattactttc attgcaagtg ccataatgta gagagtttct ctgaagtcta acagagaacc
    17101 gtttttcagt gcgcatctta actggtaaca ttagcctctt tttttaagga gcctgagatg
    17161 actaagcagc ccgaagactg gctcgacgac gttcccggtg atgacatcga agacgaagac
    17221 gatgaaatta tctgggtcag taaaagtgaa attaaacgtg atgccgagga gctaaaacgc
    17281 cttggcgcgg aaattgttga tctggggaaa aacgcgctgg ataagatccc gttagatgcg
    17341 gatctgcgcg cggctattga gctcgcccag cgtattaaga tggaaggtcg ccgccgccag
    17401 ttgcagctca tcggtaagat gctgcgccag cgcgacgtag agcctattcg tcaggcgctg
    17461 gataagctga aaaaccgtca caaccagcag gtggtgctgt tccataaact ggaaaacctg
    17521 cgcgatcgtc tgatcgatca aggtgatgac gccatcgctg aagtattgaa tctgtggccc
    17581 gatgccgatc gtcagcaact gcgtacactg atccgcaacg cgaagaaaga gaaagaaggg
    17641 aataagccgc cgaaatccgc acgccagatt ttccagtatc tgcgcgaact ggcggagaat
    17701 gaagggtaat tttcgttgtt gatgtgagta tggtttgccg gatgcggcgt aaacgcctta
    17761 tccggcaatg ctatatcagg ctgaagccga attactgcgc agcttccgcc ttcttcgcca
    17821 gaccatccag cagtttctga tggatcccac caaaaccgcc gttgctcatc accagaatat
    17881 ggtcgccagg ctgagcggtt ttcaccacca tatctgccag cgtatccaca tcgccactcc
    17941 agtgtgcagg ctgaacgcag gcttctgcca cttctgccac ctgccacgga atatgcgccg
    18001 gttgcagcag gaagacttca tcggcacgac ctaatgaagg tgccagatcg tctttgcaga
    18061 tccccatttt catggtattc gagcgcggtt ccagcacagc aataatgcgc gccgtaccac
    18121 caactttgcc acgcagcgcc gccagcgttg ccagaatcgc cgtcgggtga tgggcaaaat
    18181 cgtcatatac cgtgacgcca ttcgcttcac cacgcaactc cagacggcga cgagcattaa
    18241 taaacgaacc cagcgcgtta gcggcatctg ccgacgctac accaacatgg cgagccgctg
    18301 caatcgccat caggccattg tgcatattat gttcgcctac cagcgaccat ttcacttcgc
    18361 ccactttttc gccatccagc aaaacttccc attcggaagc atcggtggtc agctttttcg
    18421 cctgccagtg accctgctca cccaccagct cctgctcgct ccagcagccc atcgccatgg
    18481 tctgtttcag gttgatgtca ttttccggcc agataatacg gccctgcccc ggaacgatac
    18541 gcaccagatg gtggaactgt ttctggatcg ctttcaggtc gtcaaagata tcggcgtgat
    18601 cgaactcaag gttgttgagg atcagcgtac gcgggcagta atggacaaat ttagagcgtt
    18661 tgtcgaagaa ggcgcagtcg tactcatccg cttcgataac aaagaagtcg ctttcgccca
    18721 gacgcgccga aacctcaaag ttccccggca caccgccgat cacaaatccc ggtttgtaac
    18781 cacactgttc cagaatccag gtcgccattc ccgcggtggt ggttttgccg tgtgtaccgg
    18841 caacggccag cacccagcgg tcgcgcagca caaaatcgtg cagccactgt ggacctgaca
    18901 tataagggat gtttttttcc agtaccgctt ccacacacgg atttccacgg gtcatggcgt
    18961 tgccaataat caccagatcc ggctgcggct cgagctggct ggcatcgtaa ccctgaatca
    19021 gctcaatgcc ttgcttctca agtaaggtgc tcatcggcgg atacacattg gcgtccgaac
    19081 ccgttacttc atggcctaac tggcgcgcca gcatcgccag accgcccata aatgtgccac
    19141 aaattcctaa aatatgaatg cgcatacgtc actatccttt tttaatctgc cgctcatttt
    19201 acgcatatgt cgggcaagtg agaaacgcat ttcaggataa tccgcaattt gctggcgcga
    19261 ttcacctgag cgcaacattg agattatttg ttaagattgt tgcggtcgct ttactccata
    19321 aacattgcag ggaaagtttt atgaaaacgt taggtgaatt tattgtcgaa aagcagcacg
    19381 agttttctca tgctaccggt gagctcactg ctttgctgtc ggcaataaaa ctgggcgcca
    19441 agattatcca tcgcgatatc aacaaagcag gactggttga tatcctgggt gccagcggtg
    19501 ctgagaacgc gcagggcgag gttcagcaaa aactcgactt gttcgctaat gaaaaactga
    19561 aagccgcact gaaagcacgc gatatcgttg cggggattgc ctctgaagaa gaagatgaga
    19621 ttgtcgtctt tgaaggctgt gaatacgcaa aatacgtggt gctgatggac cccctggatg
    19681 gctcgtccaa catcgatgtt aacgtctctg tcggtaccat tttctccatc taccgccgcg
    19741 ttacgcctgt tggcacgccg gtaacggaag aagatttcct ccagcctggt aacaaacagg
    19801 ttgcggcagg ttacgtggta tacggctcct ctaccatgct ggtttacacc accggatgcg
    19861 gtgttcacgc ctttacttac gatccttcgc tgggcgtttt ctgcctgtgc caggaacgga
    19921 tgcgcttccc ggagaaaggc aaaacctact ccatcaacga aggaaactac attaagtttc
    19981 cgaacggggt gaagaagtac attaaattct gccaggaaga agataaatcc accaatcgcc
    20041 cgtatacctc gcgttatatc ggttcactgg ttgcggattt ccaccgtaac ctgctgaaag
    20101 gcggtattta tctctaccca agcaccgcca gccacccgga cggcaaactg cgtttgcttt
    20161 atgagtgcaa cccgatggca ttcctggcgg aacaagcggg cggtaaagcg agcgatggca
    20221 aagagcgtat tctggatatc atcccggaaa ccctgcacca gcgccgttcg ttctttgtcg
    20281 gcaacgacca tatggttgaa gatgtcgaac gctttatccg tgagttcccg gacgcgtaat
    20341 ctcctgcgcc tctccacacg gagaggcgtt tttattgctg cgcaatgttg actcttgtca
    20401 ctggcgaact ctgacgattc tcccacagca ccgttaatcc acgctgtaat gctataaaaa
    20461 taaacaacaa aataccgatg gcgattttcg tccaccagga actcagcgtg ccatcaaagt
    20521 ttatgtaagt ctgaatcagt ccctgaatcg ccacgccaaa aagcgtccct aataccgttc
    20581 caacgccacc gctcaaaagc gtaccgccaa ttaccactga ggcgatagca tccagctcca
    20641 cacctacgcc cgccagcgca tatccggcct gggtataaat cgagaagaca atccccgcca
    20701 gcgttgccag tccggtggag agcatataaa tgcgaatagt ggtgctgcga gtggaaatcc
    20761 ccatcagatt cgccgacgtt gcgttgccgc caatggcgta tacctgatta ccaaaacggg
    20821 tacgatgcgc gaggaatatg ccgataacca ccaccgccaa catcagcagt cccatcgcac
    20881 ttaagcgacc gccgccaggg attttccacg caaggcttga gagcgtgtca taaatgggat
    20941 ggtttatcgg aatcgactct tccgaaacga gatagctgac gccgcgcaaa aagaacatcc
    21001 ccgcaagcgt aatgatgaac gccgggatct tcagggcgtc gatcagaagc cccataaatg
    21061 cgccgaaggc acagcccatc accagcacca gcggaaacgc cagcagcggc gagaggccga
    21121 aatcgccaat cacttttgcc agaaacacgc cggtaaaggc gatcaccgaa ccgacggaga
    21181 gatcgatccc accggagagg atcacaaagg tcatgccaac ggcaatgatc ccaagaaagg
    21241 cgttatcggt caggatattg cagatcactc ttgtggaagc aaaaccagga aactgcgtca
    21301 ggcagtagag atagcccagc acaaagacgc cgatggtgat cataagcggc aagttacgtt
    21361 ttatcacggc tacgcactcc tttaatcaga ctgataaagc gttgcgactg aacaatcagc
    21421 acacaaagca ccaccaccgc tttcaccacc tggttcatct ctggcggaaa gcccgaaagc
    21481 aaaattccgg tattcattcc ctgaataatc agcgccccaa ctcccgaaag cagcaggtta
    21541 aagcgaccgc ccatcagcga tccaccgcca atcaccaccg cgagaatggc atccagctcc
    21601 agccataatc cggcgttgtt ggcatcggca ccgcgaatat ccgccgccac gataatgccc
    21661 gcaatcgccg cacatagccc gctcaacacg taggtgagca tgacgatgat tcgtgtgttg
    21721 actccggcat tttttgccgc ccgaatgttg ataccaacgg cttcgataaa catccccagc
    21781 gccgttttgc gggtcaacag ccagaacagg agaagcgtca gcaccgcgat aatgaccggc
    21841 gttggcagga acaacagcga tccgctgcca aaccatgaga gatccggcga gttaaacgtg
    21901 acgatctgcc cggcggtgat cagttgcgcc acgccgcgcc cggcgaccat caggatcagg
    21961 gtggcgacaa acggctgaat tttgaggatc gctaccagta tgccgttcca caatcccgct
    22021 aggatgccgg tgcccagggc gcttaacaaa acaatcggca ggctgaatcc cgcgaccgtc
    22081 atcgcagccg ttgtggctcc ggcgatagcc atcaccgccc ccacagagag atcgatacca
    22141 ccggtggcga tcaccagcgt cataccaatc gccagtaacg caacgggagc tgcacggtta
    22201 agaatgtcga tagggctacc gaacaaacgc ccatcctgaa gcaccacctg ccagaaatgc
    22261 ggggctacca ggctatcgac cagcagcacc agcaacagtg ccgccagctg cggcatcccc
    22321 gtcggccagt gaaaacgcct ttttggcggc gtagtgtcag ggagagattg aggcatcaca
    22381 ctgttctcct tacgccgcaa tggcgttcat gatcgccgga acggaaagct gtgccagcgg
    22441 gatctccgcc acctgtttgc gatcgcgcat gatgatcacc cggtcggcat agcccaccag
    22501 ctcttccagt tcggaggaga tcaccagcag cgccagaccg tcagcacaca gcgtttcaat
    22561 caggcggatg atctcggcgt gggcaccaac atcaatgcca cgggttggct catcaaggat
    22621 cagaaattgc ggtcgggtca gtagccaacg tgaaagcagc actttttgct gattgccacc
    22681 ggagagaaat tcaatcggtt gttcagttga aggtgtgcga atgccaagct ggcggataaa
    22741 gcgttcggca atctcttgct gctctttgcg ggaaatgggt cgtagccagc cgcgctgggc
    22801 ctggagagcg aggatgatat tttcccgcac cgaggcggcg gcgatgatgc catcggtttt
    22861 cctgtcttcc gggcagaatc cgatgcccag tactgaagcc tgatgtggcg atcgcagggt
    22921 ttgcggtttg cctttgatca acgccgtgcc gctgtcagca ggtttgatac cgaagatcac
    22981 ttcggcggtt tcggtacgtc cggatcccag caagccagcc agaccgacga tctcgccggg
    23041 gcgcacttcg agatcaaacg gtgctatcgt tccttttttg ccgtaatttt tgaacgcggc
    23101 aacgggtttg tcgctcaaca atgttcgccc ggcacgctgt agcacgtggg tatccagctc
    23161 gcgccccagc atcattttta ccagttcgat ctgcggtagc tcgcgcgttt cccgacagcc
    23221 tacgaaactg ccgttgcgta agacagtgat ccgatcgctg acctgataga cctgatcgag
    23281 aaagtgagtg acaaagatca ggctgacgcc gcgatcgcgc aactgacgca tcaggccaaa
    23341 taatagctcc acttcctggg tgtcgaggct ggcggtgggt tcatcgagga tcagcacttt
    23401 ggcggagaga tcgatagccc ggcaaatggc gacgatttgc tgcatcgcga ctgaaaagcg
    23461 gttaagcggt tcgcgcacgt cgaggaagaa accgtaagat gccatcagtt cggtggcgcg
    23521 cttttccatc tctttacggc gtagaaggcc gaaacgtttg ggttcgcgac ctatgaatag
    23581 attatcagca accgacatat tgggtagcag gttgacttcc tgatagacgg tgccaatacc
    23641 gagttgttgt gcatgggcgg tatttttcgg tgagatagcc tggccttcca gccagatggt
    23701 gccgcgatcg gcgtggtata caccagttaa tgctttgatt agcgttgatt ttcccgcccc
    23761 gttttcacca agcagcgcca tgatttcgcc acggcgcagg ctgaaatcaa cgttgtctaa
    23821 cgctttgacg ccggggaaaa atttacttaa tccttcggtg cggaggatct cctggtgttg
    23881 gtcggtcgtc atggtttccc ccggtgtaaa gcgtgctccc agtaggcatg ataagacgcg
    23941 gtaagcgtcg catcaggctt cagtgcacca tcgccggatg cggcgcgagc gccttatccg
    24001 gcctacgaat cggcatcagg catagcaacc ctcaataccc catatttttc ttcttctcta
    24061 actcttcttt tgcggtatca ggcaggtaaa gggtggattt ggttaacgtc agcttttcag
    24121 gcatggtgcc gtcttttttg tatttctcca gcgcatcgaa agcgggtccc gccatgttcg
    24181 gcgtcagttc aacactggcg ttcgcttcgc catccatcat cgctttgtag atgtccggta
    24241 caccgtcaat ggaacccgtg aggatatctt tgcccggttt caggcccgct tctttaattg
    24301 cctgaattgc accaatcacc atgtcgtcgt tatgggcgta aaccatgcag atgtttttgc
    24361 cgttgttttc cgctttgata aagctctcca tgacttcttt gcctttactg cgggtgaagt
    24421 cacctgactg cgagcggata attttgatat ttggcgcatt cttaatggct tcggcaaagc
    24481 ctttcttacg gtcaatggcg acgctggccc caacggtgcc ctgcagctcc accacgttgc
    24541 atggtttgcc attcacttct tttaccagcc agtcaccgat caacttacct tcgaggatgt
    24601 tgtcggcagt aacggtggtc atatagagag atttgtcttt cacatcgatg gaacggtcga
    24661 gcaagaagac cgggatttcg gcatctttcg cctcttttaa taccggttcc caacccgtcg
    24721 caaccactgg agcgataaag atcgcatcca ccccttgtgc aacgaaggaa cgtaccgctt
    24781 taatctggtt ttcctgcttt tgctgaccat cggcaatttt caacgtaatt ccgcgttttt
    24841 cggcctcact tttcgccaca ttggtttctg cggcgcgcca gccggattcc gatccgacct
    24901 gcgaaaatcc tccggttaat ggagctgcca acgccataga cgacatggct gccgagactg
    24961 cagagactac aagtaagcgt ttccacatag ggttgtcctc gtcggggtaa ttattattgg
    25021 tgaaaagatg ttcgcgaaaa aactatagac aattcgttat gtaacggatt gcgttacatc
    25081 acacttcaaa aaagtaaata aaacgttaat cacaagtttg taatcgcttt catctcacta
    25141 tgaaaaatgc ggctacggtt atggattttc ctgctctgta taccgtctta aaactggcga
    25201 aaaaggaaaa tgaagacgaa aacaagcaaa gacattcggc gcgagttggc tataatactc
    25261 ggcacttgtt tgccacatat ttttaaagga aacagacatg agcttactca acgtccctgc
    25321 gggtaaagat ctgccggaag acatctacgt tgttattgag atcccggcta acgcagatcc
    25381 gatcaaatac gaaatcgaca aagagagcgg cgcactgttc gttgaccgct tcatgtccac
    25441 cgcgatgttc tatccgtgca actacggtta catcaaccac accctgtctc tggacggtga
    25501 cccggttgac gtactggtcc cgactccgta cccgctgcag cctggttctg tgatccgttg
    25561 ccgtccggtt ggcgttctga aaatgaccga cgaagccggt gaagatgcga aactggttgc
    25621 ggttccgcac agcaagctga gcaaagaata cgatcacatt aaagacgtaa acgatctgcc
    25681 tgaactgctg aaagcgcaaa tcgctcactt cttcgagcac tacaaagacc tcgaaaaagg
    25741 caagtgggtg aaagttgaag gttgggaaaa cgcagaagcc gctaaagctg aaatcgttgc
    25801 ttccttcgag cgcgcaaaga ataaataagt tcttctagcg caataaccct gaacgccggg
    25861 cttcggttag taagggtttt ttatgcccgc gataaataaa ctctctattc caccatcatt
    25921 attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca ccattttgct
    25981 gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt caaagcgtgc
    26041 ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg attgcagata
    26101 tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa aatcgcgaga
    26161 tacatcgcat aaaacaacgc cacccgaagg tggcgtgctt ttattattac ttatccctgt
    26221 ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga taaacgtaca
    26281 tccatgcact cccgtacggc gtctgaatca actggcgcgc gtattcaccg cccctggtgc
    26341 gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca ccgtgtaccg
    26401 ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg tagttatcga
    26461 tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct tgtttgtggc
    26521 gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga gcaaatccag
    26581 tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc gataacgtaa
    26641 cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca gacctggcaa
    26701 tacatagccg ctgaccacta cctgggagga gtcgcctact ccctgggtgt cgagcgctaa
    26761 attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt gcgcaacccc
    26821 cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct ccagcccttg
    26881 tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag agaagatctc
    26941 cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcaattacgt cgtcttctgt
    27001 agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg gaccagagaa
    27061 cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc gcccttcagg
    27121 gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt taagatcgcc
    27181 cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa cgtggacaat
    27241 caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca ggttatcgtt
    27301 aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt gcaccacgat
    27361 gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg tagcctcgaa
    27421 tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc gcactttact
    27481 gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg catcaccgtt
    27541 caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc gcataccgtt
    27601 aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc cgtcgatatc
    27661 cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac cacccagacg
    27721 caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg ggtttatcat
    27781 cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc cttgcggatc
    27841 ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg tccagcccaa
    27901 ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg ccaccggcag
    27961 cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg tgatactgcc
    28021 ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg tgaagatacc
    28081 gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga agcggttgag
    28141 attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc acagttccgc
    28201 attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat tgcggtaatc
    28261 cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaaac gggtattgct
    28321 aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat taagctgccc
    28381 ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc tgcctttggc
    28441 attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa cgcgtacgtc
    28501 gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa cctgcgccac
    28561 ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt gtggcgcttc
    28621 caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg gcagcgcgtt
    28681 atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc ccagctcgcc
    28741 tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca tccactgcat
    28801 ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga cgttaccggt
    28861 cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac cgcgggtttt
    28921 aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt taatgccatt
    28981 aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg ccttgagttc
    29041 agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct gttcattacc
    29101 tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg ccgtacgcat
    29161 agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat ccgcctgata
    29221 ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca cgttgagtgg
    29281 caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg gaccggaaag
    29341 gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct tcaccttttc
    29401 acctttcaac ggctccacgt tcagtgtgct gttgaggatg atatccaccg gccagttgtc
    29461 tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga tatccagggc
    29521 gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc gcacggtgat
    29581 gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt tcaggttaag
    29641 cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat ctttcagcgt
    29701 ttcgccgagc ggcttttcat ccggctgcgg attttcaatc ttcggttcga cgacctcttc
    29761 ctgcgccact tccgccactt tcggcagagc aatcagcagg cctttcagcg acgtcggttt
    29821 cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca ccgacaccgt
    29881 ggtgtcatca atcttgatgt tgacgttatc cagcgccacc cgtgtcaggg tgatgggata
    29941 cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt cagaaggagg
    30001 cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat taatacaaac
    30061 gctactgttc cacaggcact caagaccgac cgccagatgc agattacccg cttttaccgc
    30121 cacgcctggc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc cgccggtgac
    30181 gttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa gatgcaggcc
    30241 gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa cgataaccac
    30301 gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat gtaaaactgt
    30361 aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat tggcccgacc
    30421 ggcgattccc agcgtacgcc gcccccggta ccggttttaa agtcgctgcg gcgaatatcg
    30481 cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt cacgttgtac
    30541 tggtattcca gcgatccggt tatcagcttc gaggcccctt tcaggtcacc gttggcgtat
    30601 ttcggggcga tagatttata tttgtagccg cgaatactac gatcgcctcc ggcgaagaaa
    30661 cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag cgtgccgcgt
    30721 gtcacaaaac gatggcgatc gtacagtgtg cggatccaga cgttctgcgc ctggaaaacg
    30781 gagaaatcaa catctgaacc ccaggccgtg ttggagtagt cgatagagta gcgttgcgag
    30841 tcgccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat caccccagga
    30901 taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag gctccagcgc
    30961 aggttaatgg cacgctgcca gtcgctggag agatcccagt agcgagaagc caccagcgta
    31021 gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg caccaaataa
    31081 tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag gatctgttcc
    31141 ggcgcggaaa tactggtact ggtggtcaga ctgtgaccat aagagttcat ccacggcttt
    31201 ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc ggtttcgatg
    31261 gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt ttcgcgcgct
    31321 ttatcaaatt gcggtgccac taccaccgaa ttaaaccagc cggtagcaga aagtcggcgg
    31381 ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg caccagattt
    31441 tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa gcggtaacgt
    31501 tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc aatgcccagc
    31561 tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact ggttaaggac
    31621 tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg gcgagtatcg
    31681 agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac atcggtgccg
    31741 ccaattaaca ccggtacacc tggcgtgact ttggcgatca atacctgccg ccctttcttt
    31801 ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc tttcagacct
    31861 tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac ttcatcactt
    31921 tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa cccctcgacc
    31981 tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct tacacagcat
    32041 aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctacc cctggaacgc
    32101 cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta aaccccaaat
    32161 atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa tacgcctctt
    32221 gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag tttatttgat
    32281 aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc gatgcccgta
    32341 gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg aatggagatt
    32401 gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca gttacccggc
    32461 gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac ttatcgggaa
    32521 gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga tccttccgtc
    32581 atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc ccagggaatg
    32641 cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct gacccctgaa
    32701 caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct tgccgccgat
    32761 gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta tgccgaagat
    32821 gaccaccagc aatatctgca taaaaaccca tatggttact gtggaattgg cggaattggc
    32881 gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt gataaagtgc
    32941 tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat cgtccaaatt
    33001 caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca gtattgcatt
    33061 tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc tatactagcc
    33121 actgaaaatg tcggttcact ttcttcgaat cggctttcaa tgtgtatttc acacaaatta
    33181 atcaactttc ccttccgagg atctggcctg aaaggtcgga taagatatgt taaacagtat
    33241 tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg agatctcgct
    33301 tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata taaatgccca
    33361 acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc aaatcggtct
    33421 gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc cagcttttca
    33481 cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct ttattctctc
    33541 tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga aacgcatcgg
    33601 tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct tctgcctgta
    33661 cgtttgcacc ccactggtgt ggttcttcaa cggcctggcg aacatgatct tccgtatttt
    33721 caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg cggtagtgga
    33781 agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg aaaacgtctt
    33841 tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg tgatttggtt
    33901 tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc cgcactctaa
    33961 gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt ctaaagacct
    34021 gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac aaattcgcaa
    34081 cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt ttaaaaccgc
    34141 aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga tcatcaccct
    34201 caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg aagagcagat
    34261 tgtcgcccgt gatgagaact catggctgat tgacggcggc actccgattg acgacgtcat
    34321 gcgcgtgctg gatattgacg aattcccgca gtcgggcaac tacgaaacca tcggcggctt
    34381 tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat tcgccggcta
    34441 caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg tgacccggat
    34501 cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata aagaagaaag
    34561 cgtcgcgtaa ccccccagaa acatcaacgg ctcctgaatc aggagccgtc ttattactgc
    34621 atagcacttt ggttaagcca tctctgtttg cagacgcata acctgacggt taacttcgga
    34681 catcactgac aaatgcagct tatccttcac ttttgggata agaatcttac ccttatcaaa
    34741 ttcaaaagcg ccaacgtcct tgatgtataa ccgtccacgg aacaggatct ttacgtactt
    34801 cgccacctga agtgggttgt accgttggaa aattttcatt gttatctcct gctgagtatt
    34861 acgcctgtgc ggtgccacaa tcggcccaac tattatgagg cgcaaatttt aatgcttagt
    34921 gactatagac tatccgggca atgtttccac cgcgtataac tttttttacc taaaggttac
    34981 aattattcag aattatcttt ttaccgaagc gcggtcttca gtataagcat tcatttttca
    35041 tatgatttgt gcgcttgacc gcaaactggc atcacacttg cgggaaattc gataaatagc
    35101 acatatgatt aaaactcaga cccaagtggt cggatcacct gcatatcata agaaggaaac
    35161 accatgaccc tacgcaagat tctggcactc acctgcctgc tgttgccgat gatggcttcc
    35221 gcacatcagt tcgaaaccgg tcagcgagtg ccgccgattg gcattaccga tcggggcgag
    35281 ttggtgcttg ataaagatca gtttagctac aaaacctgga acagcgcgca gttagtggga
    35341 aaagtgcgag tactgcaaca tattgctggt cgcacctctg caaaagagaa aaacgcgacg
    35401 ctgattgaag cgattaaatc agcgaagtta ccgcacgatc gttaccagac caccactatt
    35461 gttaacaccg acgacgcgat tccgggttca gggatgtttg tgcgcagcag tctggagagt
    35521 aataaaaagc tttatccctg gtcgcagttt attgtcgata gcaatggcgt cgcgcgcggt
    35581 gcctggcagc tggatgaaga aagttccgct gtagtggtgc tggataagga tggtcgcgtg
    35641 caatgggcca aagacggggc gctcactcag gaagaggtgc agcaagtgat ggacctgctg
    35701 caaaagttgc tcaaataatg gcaacaacat agattctgca aaaataattt tcccctatcg
    35761 ttatctatgg gataggggaa ttaagtctct gagttcttac gccgaactca tactggtaac
    35821 gctagttgct cttttttaaa cgcctcaatg gtcgacttat atttcttgca aatcttttct
    35881 ttggtaatat tactatctgc ggccagattc gcacttacta tcgcatccac ttgttcggct
    35941 aattccactt gttggcaacc tgcattaacc agaaaattat aaataaggga gtaatcttca
    36001 tcctccaaaa caacagagga accattacga aaacgttcat cccctccata gattttaagc
    36061 gcaaagccca aagattctat gactatggca aaattatcat caattatttt ttcgtgaata
    36121 taccatccat cgcattcttt aattccacct cctggtattt ggagttcaaa tttaccccgt
    36181 tttaatttgc ttactatatc ctccaataag gcataaccta aagacacgtc accaatcgcc
    36241 tggtgaatat aacctttaat ccttttcggt atgtcagact gttttttctg cagaatatca
    36301 caggcataca atcttctgtt ttgttctaac ggaaactcgc ggtttttgaa atccatgaat
    36361 gagttatact catttattgt tatgggaatt tcttcgtatt caaatccact acctcgcaat
    36421 gtcattgtat attcggcacc attatcttta aggctgaaac tgataaggaa cttatccatg
    36481 gcgttaacag ttccattttt tgatgaaagt acctgataac tatttttatc aatctgagtt
    36541 gcgcaacgaa agaactccct caaagttaac aggctatcat aattcgaaat ccaatctttt
    36601 ttcttaaatg catgctggat attgttaaaa aaattgtttg aatgtgtttt tgtatacgat
    36661 gactttacaa ataactccac cgcaccaata aaattattgt gtctgatcct gggtaacata
    36721 tgacaacctt attttatttg gttattttat gtcgccatta aatatctcat tagagaagta
    36781 aactatcaaa aaacgctcat tattaaaatg taacttatta gcaattgaat ttttgcactt
    36841 taaatacaat ctctttttat ttataacttt ttaggttggg agaaatcagg atggcagaat
    36901 caggaaatac actgtttctg ccatctgaat ttagtaaata gacactctga accccggatt
    36961 caggaacgac tcgcgcggag tgtaatccag cggtttaccc tgccagtcgt gaacgtgcgc
    37021 tccggcagct gcagccacag catgtccagc ggcggtgtcc caaatattcg ttggtccgaa
    37081 gcgcgggtac agctgcgcct gtccttccgc caccaggcag aatttcagcg aagagccgat
    37141 ggacgtggtc tgatgttcgc caagctgttg cagatactct ttcagctccg catctgcatg
    37201 ggaacggctg ataaccacca gcggcgggcg cgcatcgcgg acctgaatct gcttgcgcac
    37261 accgcactct tctttccagg ccttgccttc tgccgcgctg tacattacgt tcattaccgg
    37321 cgcatacacc acacctaaaa tcggtttgcc atggtcaatg agcgcaatgt taacggtgaa
    37381 ttcgccatta cgtttaataa actctttagt accatccagc gggtctacca gccagtaacg
    37441 ctgccagtgc tgacggactt cccaaccggg aggatcttct tcagaaagga ccggaatatc
    37501 cggtgtcagc gtacgtaaac cgtccatgat aacggtgtgt gcggcaatat ccgctgccgt
    37561 taccggagaa ttgtccgctt tgctgacgac gtccatcggt ttcgtcccgt cgtagacctg
    37621 cataatggca tcgcctgcat tccgtgcaag ctggcatact tgatctaaca tttctccacc
    37681 tcgtctctgt gagcggtgtt aacttattgt tttacttata ccctatcgtt aatgaatgcg
    37741 ccaactgtga tagtgtcatc attttcaaag cgtaaaattg tggcattctt cactgttcta
    37801 taagtaagac gtttattctc ccttttcttt cgtattcccg atgataaaag gatgtccctg
    37861 atgattaagt ttagcgcaac gctcctggcc acgctgattg ccgccagtgt gaatgcagcg
    37921 acggtcgatc tgcgtatcat ggaaaccact gatctgcata gcaacatgat ggatttcgat
    37981 tattacaaag acaccgccac ggaaaaattc ggactggtac gtacggcaag cctgattaac
    38041 gatgcccgca atgaagtgaa aaacagcgta ctggtcgata acggcgattt gattcagggg
    38101 agtccgctgg cagattacat gtcggcgaaa ggattaaaaa caggtgatgt tcatccggtt
    38161 tataaggcgc tgaatacgct ggattatacg gtcggtacac tcggcaatca tgaatttaac
    38221 tacggtctgg attacctgaa aaatgcgctg gcgggagcga aattccctta tgtaaatgcc
    38281 aacgtcattg acgccagaac caaacagcca atgtttacac cgtatttaat taaagatacc
    38341 gaagtggtcg ataaagacgg aaaaaaacag acgctgaaga ttggctatat tggcgtcgtg
    38401 ccgccgcaaa tcatgggctg ggataaagct aatttatccg ggaaagtgac ggtgaatgat
    38461 attaccgaaa ccgtgcgcaa atacgtgcct gaaatgcgcg agaaaggtgc cgatgtcgtt
    38521 gtcgttctgg cacattccgg gctgtctgcc gatccgtata aagtgatggc ggaaaactca
    38581 gtttattacc tcagtgaaat tccgggcgtt aacgccatta tgtttggtca tgctcacgcc
    38641 gttttcccgg gtaaagattt tgctgatatc gaaggggctg atatcaccaa aggcacgctg
    38701 aatggtgttc cggcggtaat gccgggcatg tggggcgatc atcttggggt ggtcgactta
    38761 caactcagta atgacagcgg taaatggcag gtgacgcagg cgaaagcgga agctcggccg
    38821 atttacgaca tcgccaataa aaaatccctc gcggcggaag acagcaagct ggtagaaaca
    38881 ctcaaagccg atcacgatgc cacacgccag ttcgtcagca agccaatcgg taaatctgcc
    38941 gacaatatgt atagctatct ggcgctggtg caggacgatc cgaccgtgca agtcgtgaac
    39001 aacgcgcaaa aagcgtatgt cgaacattac attcagggcg atccggatct ggcaaaactg
    39061 ccggtgcttt cagctgccgc accgtttaaa gttggtggtc gcaaaaatga cccggcaagc
    39121 tatgtggagg tggaaaaagg ccagttgacc ttccgtaatg ccgccgatct ttatctctac
    39181 cccaatacgc tgattgtagt gaaagccagc ggtaaagagg tgaaagagtg gctggagtgc
    39241 tccgccggac agtttaacca gattgatcct aacagcacga aaccacagtc actcatcaac
    39301 tgggatggtt tccgcactta taactttgat gttattgatg gtgtgaatta tcagattgat
    39361 gttacccagc ccgcccgtta tgacggcgag tgccagatga ttaataccaa tgcggaaagg
    39421 attaagaacc tgacctttaa tggcaagccg attgatccga acgccatgtt cctcgttgcc
    39481 accaataact atcgcgctta cggcggcaaa tttgccggta cgggcgacag ccatatcgct
    39541 tttgcttcac cggatgagaa ccgctcggtg ctggcagcgt ggattgctga tgagtcgaaa
    39601 cgtgcggggg aaattcaccc ggcggcagat aacaactggc gtttagcacc gatagctgcc
    39661 gataagaaac tggatatccg tttcgaaact tccccgtcag ataaagccgc agcgtttatt
    39721 aaagagaaag ggcagtatcc gatgaataaa gtcgcgaccg atgatatcgg gtttgcgatt
    39781 tatcaggtgg atttgagtaa gtaaaacact tctttttcgg cctataaatc atcaatcgca
    39841 tccggcattt attggcggat gcgatgctgg cgcatcttat ccgccctaca agtcatacac
    39901 cgtaggccgc gcatccggca gttatgccgc acgttcatcc cgcaccgcca acacctcggg
    39961 caaattcaac tcaatccagt ccgccagtgc agcaaccttt tcgctcacct gctcgcccag
    40021 cggcgtgagg ctgtattcaa catgcggtgg caccaccgga tacgcgatac ggttaagaaa
    40081 accatcctgt tctaacgcct gtaacgactg cgcaagcatc ttttcgctca ccccgccgat
    40141 tttgcgccgc aggtcgctaa agcgatgagt accttcgcgt agcgccacca gaatcaacac
    40201 cccccaacgg ctggtgacgt gtttcaacac ctcgcgcgac gggcactgtt ccgcaaagag
    40261 attaccctct ttcagttgtt gcgacaggct aacctgactc atttcatact tacctttttg
    40321 tacgtactta ctaaaagtaa gtttaggtgt tagcgtattt aaacacaaga caaaacgatg
    40381 gagacttccc atgatcgcta ttactggtgc cactggccaa cttggtcact atattattga
    40441 atccttgatg aaaacggttc ctgccagcca aatagtggct atcgttcgta atccggcaaa
    40501 agcccaggct ctagcagcac aaggcattac cgtgcgtcag gctgactacg gcgatgaagc
    40561 cgcactgaca tctgcacttc agggagtgga aaaactactg ctgatctctt ccagcgaagt
    40621 gggtcaacgt gccccgcagc atcgtaatgt tattaatgcc gcaaaggcgg ctggcgtgaa
    40681 atttatcgct tataccagcc tgctacatgc agatacctcc ccgctcggcc tcgccgatga
    40741 gcacattgag acggagaaaa tgctggctga ttctggcatc gtttacaccc tgctgcgcaa
    40801 cggctggtac agcgaaaact acctcgccag cgccccggca gcactggaac acggcgtatt
    40861 tatcggtgcg gcgggcgatg gcaaaatcgc ctcagcaacg cgggcagatt atgcggcagc
    40921 tgcggcacgc gtgattagcg aagccggtca cgaaggcaag gtttacgaac tggcgggcga
    40981 tagtgcctgg acgttgacac agttagcggc agagctgacc aaacagagcg gcaaacaggt
    41041 tacctatcaa aatctgagcg aagccgattt cgccgcggca ctgaaaagcg tcggactgcc
    41101 cgacggactg gcggatatgc tggcggattc tgacgttggc gcatcgaaag gtggtctgtt
    41161 tgataacagc aaaacgctta gcaaattgat tggccgccca acgacaacgt tagccgaaag
    41221 cgtaagccat ctttttaatg ttaataacta gttaattaaa gtggcatcct cccgcatcct
    41281 ctctgataat gacgggatgc cgggagcaat catgtctgct tcctgaactt tcttctgaca
    41341 gaccaatgga tgccagtaat gattagcggc gtgctgtacg ccctgttagc agggttgatg
    41401 tgggggctta tttttgtcgg gccgttgatc gtaccggaat acccggcgat gttgcagtcg
    41461 atggggcgtt atctggcgtt agggttaatt gcgctgccca ttgcctggct gggacgcgtg
    41521 cgtctgcgtc agttggcgcg tcgcgactgg cttaccgcct tgatgctcac catgatgggc
    41581 aaccttatct attacttctg ccttgccagt gccattcaac gtactggcgc gcctgtttcc
    41641 acgatgatta tcggcaccct gccggtggtg attcccgttt ttgccaatct gctttatagc
    41701 cagcgcgacg gcaaactcgc atggggaaaa ctcgccccgg cactggtttg tattggcatc
    41761 ggcctggcgt gtgtgaatat tgctgagtta aaccacggac tccccgattt tgactgggca
    41821 cgttatacct ctggcatcgt gctggcgtta gtttccgtgg tctgctgggc gtggtatgcc
    41881 ctgcgcaacg cccgctggct gcgggaaaat cccgacaaac atccgatgat gtgggcaacg
    41941 gcgcaggcgc tggtcacact gccggtttct ctcatcggct atctcgtcgc ctgttactgg
    42001 ctgaatatac aaacgccgga cttctcctta ccttttggcc cccgtccgct ggtgtttatt
    42061 agtctgatgg ttgcgatagc cgtgctttgc tcatgggttg gcgcactctg ctggaacgtc
    42121 gccagccagc gattaccgac agtgattctc gggccgctga ttgttttcga aacgctggca
    42181 ggtttgctgt acaccttttt actccgccag caaatgccgc cgctaatgac gctgagcggt
    42241 atcgcgctgt tagtgattgg cgtggtcatt gcggtcagag caaaaccaga aaaaccttta
    42301 actgaatctg tctcagaaag ttgacacgct ggcagtgagt taaataagcc tctgctacgt
    42361 aagggttata gcttttgcct taaagatgca tttaaaatac atcttatatt attaagaatg
    42421 aggtatcagc tatggcttat cgcgaccaac ctttaggtga actggcgctc tctattcctc
    42481 gcgcttcagc tctgtttcgt aaatatgata tggattactg ctgtggcggt aagcaaacgc
    42541 tggcgcgcgc ggcggcacgt aaagaactgg atgttgaggt cattgaagct gaactggcaa
    42601 agcttgctga acaaccgatt gagaaagact ggcgtagcgc cccgctggca gaaatcattg
    42661 accatatcat cgtgcgctac cacgatcgtc accgcgagca actgccggag ctgatcctgc
    42721 aagcgactaa agtcgagcgc gttcacgccg acaaaccgag cgtgccaaaa gggctgacaa
    42781 aatacctgac tatgctgcat gaagagctat ccagccacat gatgaaagaa gagcagatcc
    42841 tcttcccgat gatcaaacaa ggcatgggca gccaggcaat gggaccaatc agcgtgatgg
    42901 aaagcgagca cgatgaagcg ggcgaactgc tggaagtgat taaacacacc accaataacg
    42961 tcacaccgcc gccggaagcc tgcaccacct ggaaagcgat gtataacggt attaatgaac
    43021 tgattgatga cctgatggat cacatcagcc tggaaaacaa tgtactgttc ccacgcgcgc
    43081 tggcgggtga gtaatctaaa agctggatgg cattgcgcca tccagcatga taatgcggtt
    43141 atttccgcag ttcagcagcc cgcttcttac caataaacaa ccagcccagc cccagtgcga
    43201 taaaccacag cggggtgacc aacagcgcct ggcgagtgtc atcttccagt gtcagcaaca
    43261 cgaccacgaa cacaaagaac gccatacata cccagcacat cagcttgccg agcggcatct
    43321 tgtagatcga cttctcatgc agatgaggac gctgtttgcg gtaaaccagg tacgagcaaa
    43381 ggataattgt ccagacgaac ataaacagaa tcgcggaaac ggttgtaatc atcgtgaacg
    43441 cgccaatcac gctaggattc acatacaaca tcaccacgcc gccgagcaga cagatacacg
    43501 agaaggtcaa ccctttcgcg ggtactgcgc gcttagaaag tttagcgaac gctttcggtg
    43561 ccacaccttc ctgcgccaga ccaaacagca tacggctggt agagaagacg ccgctgttag
    43621 cggaagacgc cgcagaggtc agcaccacaa agttgatcac gctggcagcc gcaggcaaac
    43681 ctaccaacac aaacagttca acaaacgggc tcttctccgg gactaccgaa ctccacggcg
    43741 tcacggacat aatcacaatc agcgcgaaga cgtagaacat aatgatacgg atcggaatgg
    43801 agttaatcgc gcgtggcagt gatttctccg gatctttggt ttccgcagcg gttgtaccta
    43861 ccagctcaat ccctacgaaa gcgaaaaccg ctatctggaa tccggcaaag aagccactta
    43921 aacctttcgg gaaccagccg ccatcattcc acaaatgcgc gaatgacgct tccacaccag
    43981 tcggtgactg aaagtgcatc gccaccataa ccaggccgac gacaatcagc gacacaatgg
    44041 cgacgatttt gatcatcgca aaccagaact ccatctcacc gaacattttc acggtggcga
    44101 gattgagcgt cagcagcagc actatcaccg ccagcgaggc gacccagtcg gagagatcgg
    44161 ggaaccagaa ctgagcataa gcagtgatcg ccaccacgtc tgccataccg gttacaaccc
    44221 agcagaacca gtaagtccag ccggtgaaat atcctgccca cggcccgagt aaatcggaag
    44281 cgaagtcact aaaagatttg tattccagat tcgaaagcag caattccccc attgcccgca
    44341 tcacgaaaaa gagcataaaa ccgatgatca tataaacgaa aatgatcgac ggcccggcaa
    44401 ggctaatcgt tttgccggac cccataaaca accccgtacc aatggcaccg ccaatggcaa
    44461 taagctgaat atgtcggttt gtgagattgc gccgtagcga ctgttcagcc ggagcctgat
    44521 catcggcaac gacttttacc tgatctacca tgtttttttc ttcctgtacc tgtctgtgtt
    44581 gttcaggctc tacggccttt agtcagtcta tgatacgacg aatctgtatc gggactcatc
    44641 gatattaggt aacaatacgc gggatgaata ctaagattta gatctaatgt taattttatg
    44701 tttaaagtga gtgtcatatc acggtgataa cgcgaaacag ctcacaaaaa tacacgctta
    44761 cggggtcttt gcaacacaaa atagcgccgt ttggcaaact gaagggttta ttgctgaatg
    44821 cctgctcccc tctggttcga ggggaacaga atgcgctgct tagaggattt ccagcagttc
    44881 gacttcaaac accagggtgc tgaacggagg gatggatgcg cctgcgccgc gctcgccata
    44941 tgccagttcc tgcgggatag tcagttccca tttagaacct accggcatca gagtcagtgc
    45001 ttcaatccag ccagggatca cgccattaac cgggaattca gcgggttcac cacgagcaac
    45061 ggagctgtca aacacggtgc cgtcgatcag tttaccggtg taatgaacac gaacgcggtc
    45121 ggtacgtgcc ggaattgcgc cttcaccctg gttgatcacg cggaattgca ggccagattc
    45181 ggtgctattc acaccttctt ttttggcgtt ttcttccagg tatttcacac cttcagcagc
    45241 catcgcctgg aaacgctgac gacgaacggc atcggcgcgt tcgtggattt cacgcagcgc
    45301 gcgatgcacc acatcaaccg gcacagccgg atgtttgcct tccagcgcat cggcaatacc
    45361 tgcaaccagt gcttctggca gcagcccttc caggccagat tcactcagtt gttgccctac
    45421 ctgcaaacca atgccgtagc ttgcttgcgc ttcgatggtg tcaaaagttg gggtggtcat
    45481 cgtatttcct ttcttagggt ataaaagtag cgggcagcat atcagccaca cttctttggg
    45541 taaaactttg tcgcaggaat gatgacaaat cccggggaaa aagaaacaat agcaatatcc
    45601 cgtatgaata taataaaggc gggtttacca cggacatcat agcgttagct tattcatagg
    45661 ctatgattga ggaacaagac gcggagcagg aggaaaccat gcccgggcgc tttgaactaa
    45721 aaccaaccct ggagaaagtc tggcacgcgc cggacaattt tcgcttcatg gacccgctgc
    45781 cgccgatgca tcgtcggggg atcattattg ccgccattgt attggtggtc ggatttctgc
    45841 ttccatctga tgatacgccc aacgcgccgg tggtgacgcg tgaagcgcag ttagatattc
    45901 agtcgcaatc acaaccgccg acagaagaac agctgcgggc gcaattagtc acaccacaaa
    45961 atgatcctga tcaggtggct ccggtcgctc cagaacctat acaggaaggc cagccagaag
    46021 aacagccgca aactacccaa acgcaaccat tccagccaga tagcggaatt gataatcaat
    46081 ggcgctctta ccgtgtcgaa cccggtaaaa cgatggcgca gctgttccgc gaccacggac
    46141 tgcccgctac cgatgtctat gcaatggcgc aggtagaagg cgcgggtaag ccgctgagta
    46201 atttgcaaaa tgggcagatg gtgaaaatcc gccagaacgc cagcggcgtg gtgacgggct
    46261 taaccattga tacggggaat aatcaacagg tcctgtttac ccgccagccg gatggcagtt
    46321 ttattcgtgc gcggtagatg aagggtcaac caaaagccag ctaaggattg tctctttgat
    46381 gaccttacgt ctggcttcca gtgcctgggg tgaaaccata ctgatgtgat aaaccaggcc
    46441 aagcgtatgc tggttgatca aataatatcc cccaagcgca gcaatattaa tattcacctg
    46501 caatgggtcg atatctggct taaaaatatt gtgtttcttc ccttcatcca ataaggattc
    46561 cattaactgc aagtgcgcat ggttaatttc taagagacgc agtgattttg cataatgcac
    46621 acctttactt tgattttcac tatgcacaat ttttaaaaac caagggt
//
LOCUS       DAEEKQ010000011        45728 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.011, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000011 DAEEKQ010000000
VERSION     DAEEKQ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.011"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(87..1946)
                     /gene="gss"
                     /locus_tag="KLK57_000661"
     CDS             complement(87..1946)
                     /gene="gss"
                     /locus_tag="KLK57_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HBE0891127.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            2151..3017
                     /gene="yghU"
                     /locus_tag="KLK57_000662"
     CDS             2151..3017
                     /gene="yghU"
                     /locus_tag="KLK57_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HBE0891128.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(3140..3388)
                     /gene="hybG"
                     /locus_tag="KLK57_000663"
     CDS             complement(3140..3388)
                     /gene="hybG"
                     /locus_tag="KLK57_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HBE0891129.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(3401..3742)
                     /gene="hypA"
                     /locus_tag="KLK57_000664"
     CDS             complement(3401..3742)
                     /gene="hypA"
                     /locus_tag="KLK57_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HBE0891130.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(3735..4223)
                     /gene="hybE"
                     /locus_tag="KLK57_000665"
     CDS             complement(3735..4223)
                     /gene="hybE"
                     /locus_tag="KLK57_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HBE0891131.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            complement(4216..4710)
                     /locus_tag="KLK57_000666"
     CDS             complement(4216..4710)
                     /locus_tag="KLK57_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HBE0891132.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(4710..6413)
                     /gene="hybC"
                     /locus_tag="KLK57_000667"
     CDS             complement(4710..6413)
                     /gene="hybC"
                     /locus_tag="KLK57_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HBE0891133.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(6410..7588)
                     /gene="hybB"
                     /locus_tag="KLK57_000668"
     CDS             complement(6410..7588)
                     /gene="hybB"
                     /locus_tag="KLK57_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HBE0891134.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLKRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(7578..8564)
                     /gene="hybA"
                     /locus_tag="KLK57_000669"
     CDS             complement(7578..8564)
                     /gene="hybA"
                     /locus_tag="KLK57_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HBE0891135.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(8567..9685)
                     /gene="hybO"
                     /locus_tag="KLK57_000670"
     CDS             complement(8567..9685)
                     /gene="hybO"
                     /locus_tag="KLK57_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HBE0891136.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(9874..10161)
                     /locus_tag="KLK57_000671"
     CDS             complement(9874..10161)
                     /locus_tag="KLK57_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HBE0891137.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(10280..11167)
                     /locus_tag="KLK57_000672"
     CDS             complement(10280..11167)
                     /locus_tag="KLK57_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBE0891138.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYIMYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            11324..12364
                     /gene="gpr"
                     /locus_tag="KLK57_000673"
     CDS             11324..12364
                     /gene="gpr"
                     /locus_tag="KLK57_000673"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HBE0891139.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRIGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(12404..12898)
                     /locus_tag="KLK57_000674"
     CDS             complement(12404..12898)
                     /locus_tag="KLK57_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HBE0891140.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            13089..13973
                     /gene="yghA"
                     /locus_tag="KLK57_000675"
     CDS             13089..13973
                     /gene="yghA"
                     /locus_tag="KLK57_000675"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBE0891141.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(14133..14558)
                     /gene="exbD"
                     /locus_tag="KLK57_000676"
     CDS             complement(14133..14558)
                     /gene="exbD"
                     /locus_tag="KLK57_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HBE0891142.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(14565..15299)
                     /gene="exbB"
                     /locus_tag="KLK57_000677"
     CDS             complement(14565..15299)
                     /gene="exbB"
                     /locus_tag="KLK57_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBE0891143.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            15551..16738
                     /gene="metC"
                     /locus_tag="KLK57_000678"
     CDS             15551..16738
                     /gene="metC"
                     /locus_tag="KLK57_000678"
                     /EC_number="4.4.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HBE0891144.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDCVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCVLFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLSNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            16878..17537
                     /locus_tag="KLK57_000679"
     CDS             16878..17537
                     /locus_tag="KLK57_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0891145.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(17577..18476)
                     /gene="yqhC"
                     /locus_tag="KLK57_000680"
     CDS             complement(17577..18476)
                     /gene="yqhC"
                     /locus_tag="KLK57_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HBE0891146.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVDYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            18670..19833
                     /gene="yqhD"
                     /locus_tag="KLK57_000681"
     CDS             18670..19833
                     /gene="yqhD"
                     /locus_tag="KLK57_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HBE0891147.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKI
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANDVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            19938..20765
                     /gene="dkgA"
                     /locus_tag="KLK57_000682"
     CDS             19938..20765
                     /gene="dkgA"
                     /locus_tag="KLK57_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HBE0891148.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKAPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            20965..21891
                     /locus_tag="KLK57_000683"
     CDS             20965..21891
                     /locus_tag="KLK57_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HBE0891149.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            21942..22199
                     /gene="yqhH"
                     /locus_tag="KLK57_000684"
     CDS             21942..22199
                     /gene="yqhH"
                     /locus_tag="KLK57_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HBE0891150.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDIKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(22242..24461)
                     /locus_tag="KLK57_000685"
     CDS             complement(22242..24461)
                     /locus_tag="KLK57_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HBE0891151.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSPRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCVRALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLHCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRPALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(24572..25984)
                     /gene="ftsP"
                     /locus_tag="KLK57_000686"
     CDS             complement(24572..25984)
                     /gene="ftsP"
                     /locus_tag="KLK57_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HBE0891152.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYDVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(26059..26796)
                     /gene="plsC"
                     /locus_tag="KLK57_000687"
                     /gene_synonym="parF"
     CDS             complement(26059..26796)
                     /gene="plsC"
                     /locus_tag="KLK57_000687"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HBE0891153.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(27029..29287)
                     /gene="parC"
                     /locus_tag="KLK57_000688"
     CDS             complement(27029..29287)
                     /gene="parC"
                     /locus_tag="KLK57_000688"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HBE0891154.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKVAVKGKSNQSVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASNGDSEE"
     gene            complement(29833..30315)
                     /locus_tag="KLK57_000689"
     CDS             complement(29833..30315)
                     /locus_tag="KLK57_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0891155.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(30368..30760)
                     /gene="ygiW"
                     /locus_tag="KLK57_000690"
     CDS             complement(30368..30760)
                     /gene="ygiW"
                     /locus_tag="KLK57_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HBE0891156.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            30912..31571
                     /gene="qseB"
                     /locus_tag="KLK57_000691"
     CDS             30912..31571
                     /gene="qseB"
                     /locus_tag="KLK57_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HBE0891157.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            31568..32917
                     /gene="qseC"
                     /locus_tag="KLK57_000692"
     CDS             31568..32917
                     /gene="qseC"
                     /locus_tag="KLK57_000692"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HBE0891158.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHSIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVVDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            33027..33608
                     /gene="mdaB"
                     /locus_tag="KLK57_000693"
     CDS             33027..33608
                     /gene="mdaB"
                     /locus_tag="KLK57_000693"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HBE0891159.1"
                     /translation="MSNTLIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            33639..33953
                     /locus_tag="KLK57_000694"
     CDS             33639..33953
                     /locus_tag="KLK57_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HBE0891160.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(34001..35893)
                     /gene="parE"
                     /locus_tag="KLK57_000695"
     CDS             complement(34001..35893)
                     /gene="parE"
                     /locus_tag="KLK57_000695"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HBE0891161.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPNSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(35922..36503)
                     /gene="yqiA"
                     /locus_tag="KLK57_000696"
     CDS             complement(35922..36503)
                     /gene="yqiA"
                     /locus_tag="KLK57_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HBE0891162.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(36503..37330)
                     /gene="cpdA"
                     /locus_tag="KLK57_000697"
     CDS             complement(36503..37330)
                     /gene="cpdA"
                     /locus_tag="KLK57_000697"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HBE0891163.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(37355..37777)
                     /locus_tag="KLK57_000698"
     CDS             complement(37355..37777)
                     /locus_tag="KLK57_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HBE0891164.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(37778..38407)
                     /gene="nudF"
                     /locus_tag="KLK57_000699"
     CDS             complement(37778..38407)
                     /gene="nudF"
                     /locus_tag="KLK57_000699"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HBE0891165.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            38612..40093
                     /gene="tolC"
                     /locus_tag="KLK57_000700"
     CDS             38612..40093
                     /gene="tolC"
                     /locus_tag="KLK57_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HBE0891166.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            40241..40912
                     /locus_tag="KLK57_000701"
     CDS             40241..40912
                     /locus_tag="KLK57_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HBE0891167.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            40918..42078
                     /locus_tag="KLK57_000702"
     CDS             40918..42078
                     /locus_tag="KLK57_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBE0891168.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKRTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFIE"
     gene            complement(42116..42904)
                     /gene="ygiD"
                     /locus_tag="KLK57_000703"
     CDS             complement(42116..42904)
                     /gene="ygiD"
                     /locus_tag="KLK57_000703"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HBE0891169.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLASVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            43047..43820
                     /gene="zupT"
                     /locus_tag="KLK57_000704"
     CDS             43047..43820
                     /gene="zupT"
                     /locus_tag="KLK57_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HBE0891170.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(43878..44048)
                     /locus_tag="KLK57_000705"
     CDS             complement(43878..44048)
                     /locus_tag="KLK57_000705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891171.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(44309..44962)
                     /gene="ribB"
                     /locus_tag="KLK57_000706"
     CDS             complement(44309..44962)
                     /gene="ribB"
                     /locus_tag="KLK57_000706"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HBE0891172.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(45066..45214)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            45336..45626
                     /gene="ubiK"
                     /locus_tag="KLK57_000707"
     CDS             45336..45626
                     /gene="ubiK"
                     /locus_tag="KLK57_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HBE0891173.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLARLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT        11298 a        12258 c        11468 g        10704 t
ORIGIN      
        1 tattagaaca tataaatatt gaatattttg attaatcccc gtactgatta ttcttcataa
       61 tcagtacggg gttgctacaa catgtattac tttttcacca caattaacgg ttcaatatca
      121 ctctcttttt tgatgaccag tgattcatca ccgcgcaaac acgtcccacc gtagttgccg
      181 cccacggtga aggtacatac ctgaatgtat ttaccgtcca ctttcggcaa acaccacagt
      241 tgctgataga tgtttttctg ctcggcaaat ttaccgctgg ttttgtccag cacctcttca
      301 tgatggctga ccaggtcgat attgctacca cagcgaccgg caatcggttt cactgcgtaa
      361 cctgttttca ccaattcatc attaacagtg aaatcggtat ccagcaggta acgatggtgc
      421 gggaacagcg accagaggat cggcagaatc gctttgttgc cggggatcac cgtccacagc
      481 ggctcaaaga ctagcacttc cgggcgcagc aatacgtcga taagacgcac ttcgttttgc
      541 ggatgaccgg tacggattgg caccgcagca aactcacggt cgctaacttc acgaatctga
      601 tcaaacgcgg tttcccacgc ccaggttttc cacacgcagt taaccagtcg cccttcccca
      661 tcaatcagtt gcccggcagc atcccagccc agttcatcca atccacgcaa gatacgcgtt
      721 tcaaagcccg cctggtgcag cgcctgctcc ataaactgcg cgtgatagtt ttcctcgata
      781 tctttgtcct gcatgatatg gacaaacgga cgtgcacgac tgtgtttcca ggcaccagcc
      841 aattcgttaa tcagcccttc cgccggattg aagccgttgc ctttatagcc ctgctccgcc
      901 caacgttcga ggatcaagcc cgcttcggta tgacaggagg cggagtcggc gttgtactcg
      961 taaaccttca ggccacgctc atccatgcag aaatccatac gaccagtgat catatggtga
     1021 cggcgacgct gccaggagag acgcaaacgt ggccagagga ttttcgggat gtcgaacagc
     1081 gccagcaggt tgtcatcttt cagcaccttg tcggttgcgt gaagatacat caggtgcagc
     1141 tcgttggtgg ctttaattag ctcctgctcg gcactctcgg taatggtgta gtaatgataa
     1201 ggatcctgat tgatcacctg accgttggcc tgcacatagg cgttttgcag cggatctttt
     1261 tcatccagcc atttaccgtc aaactggcct ttgttttcca ggcgcgctcc gctgattttc
     1321 agcagctcgc ctgcaatttc cggctgcggt aagctgtatt cagtatcttc cgtctggatc
     1381 atccagccca gaatggtggt gtcatcaaaa gtgtctttca gggtatagca gccgttttcg
     1441 accaccatct ccagctcacg cgtccactgt tgcccttgcg gcaacgggga atgaatcacg
     1501 ttctgttccg caatacggac tttgttgcca tgcaattggg taatgatggc gacatggcca
     1561 gtgtctttaa attcaccgcc tttatcccag ataagaagcg cacccgcgac cggcgcacgc
     1621 ggcgagccgt taggaaatgc ctgcaatggc aggatgttgt cattaaccac ttctcgcagg
     1681 aagcgcagcg agaaaatctc ccacgccata cccacgtcag taaagaccac accgtaattc
     1741 agaaagagaa aacggcgagc aaattcaacg cattgccact tgtggcccat atattcgtcg
     1801 tcgatatagc tacggaatac ggcgtcatct tcgtattcct gcggatcgag agaactgtaa
     1861 tctgaagagt agattgctac cccacctggg gcgtagccca ataatgtccc gaacggggca
     1921 tcctggctgg tcgttccttt gctcatcact ttacctttaa caatacaacc taagcagttg
     1981 gcgtaattgt tgtgctctga ttacctgctc agcgacatta accggacaga ggtcagacta
     2041 atcatacacc tcatcgcagc ggctggcgca aagggttaaa aaattcacat tctgtacagc
     2101 aagtgacctg ctacactgct tcaacactac cactcagaag gcaactcact atgacagaca
     2161 atacttatca gcccgcgaaa gtctggacgt gggataaatc cgctggcggc gcgttcgcca
     2221 atatcaatcg cccggtttct ggtccgacgc atgaaaaaac gctgcccgtt ggcaaacacc
     2281 cattgcaact ttattcgctg ggaacgccga acggtcagaa agtaacgatt atgcttgagg
     2341 agctgctggc gctgggcgtt actggtgcag agtacgacgc ctggctgatt cgtattggcg
     2401 atggcgatca attctccagc ggctttgtcg aagtgaaccc aaactcgaag atcccggcgc
     2461 tgcgcgatca tacgcataat ccgccgatcc gcgtgtttga atctggttcg atcctgcttt
     2521 atctggcgga gaaatttggc tacttcctgc cgcaggattt ggcaaagcgt actgaaacga
     2581 tgaactggct gttctggtta cagggcgcgg caccgttcct cggcggtggt tttggtcact
     2641 tttaccatta cgcgccgatg aaaattgagt acgccatcaa ccgctttacc atggaagcca
     2701 aacgtctgct cgacgtgctg gataagcaac tggcgcagca taagtttgtt gcgggcgatg
     2761 agtacaccat tgcggatatg gcgatttggc cgtggtttgg caatgtggtg ttaggcggcg
     2821 tgtatgatgc cgcagagttt cttgatgcgg gcagttataa gcatgtacaa cgctgggcga
     2881 aagaagtagg cgaacgtccg gcggtgaaac gtgggcgtat tgttaaccgc accaacggac
     2941 cgctgaatga gcagttgcat gagcgccatg acgccagtga tttcgagacg aatacggaag
     3001 ataagcgtca ggggtaaggg ttggtgttcg tcgcagcaag ccatccaggc cggataaggc
     3061 gttcacgccg catccggcaa tcgtgcacaa tgcctgatgc gacgctgccg cgtcttatca
     3121 ggcctacgaa agctaatcat cacgcgctgg taatgtcgta atccatttgg cgcagtgcgt
     3181 ctaatgtggc tttggcttca tcttcgtcga tgatgctcat ggcaaatccg acgtgtacca
     3241 gcacccactg gcccagtaga tcggcagggt taccttcaca aatcagggcg atattcacgt
     3301 cgcgcttgat accacatact tcaacctgcg caagctggtg aatatcttca ccgacagcca
     3361 gcacctggcc tggaacgcca atacacatta ttaactccgg ttattcaact tcaatacttt
     3421 tgacgatcag cgaatcgccg gtatcgacac gcaaccgctc gccgtgacag agcggacact
     3481 gcgcatcgtg ctgatgaatc tccaccacct ggctgcaatc ccagcaccaa gcctgggcgg
     3541 gtttatagac gatatgtaaa tcgcaccctt gcgccaccgt tccgtggcag acaatttcaa
     3601 aactaaaacg gacggcgctc tcctcaacgc aggagagcgc gccaatttcc agccacacgg
     3661 cggtgacgcg cttaacatcg tgctgctccg cctgccgttg gataatttca acggcgctct
     3721 ggcaaagaga caactcatgc attttcgcca ctcctgcgac caaacagcag ggcgcgacgc
     3781 cctgcgtgtg gtacatccgg attcgtgact ggcagcgaaa ggatcattcg tgcgcagtca
     3841 tccgtcaggc gttggccctc ttcaatcgac atgctgtgcg aaagcggcga catcagcgaa
     3901 caggagagat attgcgaaac accgtccagt tcgccaacgg taaaggtcat agtgccatac
     3961 ggcagttgca gaccaatttt ttcactcact ttgcgcagcg gccagagttg atccgggccg
     4021 gggaagataa ctgcactcag catccacggg gtgatcacac accccgtcca ctgaccttcg
     4081 aacagcgtaa aatcagaaac atacaccggc attgaaggat gcagaaaaga gagatcgtgc
     4141 atcgaacgcc gggcaatttc ttcaaacgct gcctgtactt gcgccttcgg ggaggtctgg
     4201 aaacctgcta tctcttcagt catgaatcgc ctcccgtggg atggcttcca cgcctgattc
     4261 gcgcagcgca gccagaacct gctcaagcgc aggttcaatc attgcttcaa ccgtcggcgt
     4321 taagccgatg tgtggctcca gcgattccgg gatcacgccg accagggtca gctttttcgg
     4381 aaactcaccg gtgaagcgca gggccgacaa tacgtcggcc aggccaagct gatgcggaga
     4441 gattttgttg gtaaacaacg ccggaacttc ttcatcccgc aggatcatca tcgttcccgg
     4501 cgtgttcttt ttcgacacaa tggcatccgc aataatcaga tgatcgcgat ttgccatgtc
     4561 gccaagcagc tccattcccg ccgtgccgcc atcgaggatc tcaacataat ccggcagaat
     4621 gtatcgttgc tctaacgctt cgacaatccg cacaccgatg gcttcatcgg tcagcaaaat
     4681 attgccgacc cctaagacta aaatacgcat tacagaacct tcactgaaac cacttcgttg
     4741 ccgtcggcat ccactacgtg taccgcacag gccatgcacg ggtcgaagga gtgaatggta
     4801 cgcaccactt ccagcggttt attcggatca gcaaccggtg tacccaccag cgactgctcg
     4861 taaggaccga cgtcatcatt gaagttacgc ggaccagagt tccaggttga tggaacaact
     4921 gcctggtagt tactgataat accgtcttta ataaccatcc agtgagagag cataccgcgc
     4981 ggcgcttcga ggaagccaac acctttgaac tcacccgttg ccggaatgtt cggtttcaca
     5041 aaggtggtgt gatcgccttt gccgatattg gtgatcagtg cactgtattg attttgcagg
     5101 atatcctgca attcgcagca gtgaacggta cgaccaataa tacggcccag cgtggagtgc
     5161 agctgcgcca cttccagcgt gttgccagtc agtttctgat aaatcgcaac gatttcattc
     5221 agtttgttct gggtagattc gcgacctgcc gccagtttca ccagcatatt agccagcgga
     5281 cccacttcta ccgttttgcc gtagaaagtc ggtgatttca cccaggaata tttcccgtcg
     5341 tcagaccaac catcataagc cggaatggtg gtaccttccc acggtgcctg cggcgcttcg
     5401 tctttatacc aggagtgctt cgcgctttcc tgaatgcctt tgatcaggta ttcatcggaa
     5461 tgagaagtga tcggacgata cgaggacaga tccgcattct caatgtagcc gcccgggaac
     5521 aggaagctgc cgtttttact gtcggtcggg aattccggca cgctcaggta gttcaccgca
     5581 cctttaccgc gcgtcagcca ttccgggtag aacgcggcaa taactgcggt atcaacctta
     5641 taaacctgtt caacaaagtc gctcagcttg tcgatgaaag acttgatgta catcaggcgc
     5701 tcaaggttca gcacgcccaa accgtcgagg ttgatcgggt tcgcgacacc acctaccgcc
     5761 aggttctgaa tgtgcggcgt tttaccgccc agcagcgcca cgacgcggtt agcgtcacgc
     5821 tggcactcca gcgcctgcag gtagtgcgct accgcaatca ggttcacttc cggcggcagt
     5881 ttcatcgccg ggtgacccca gtagccattg gcgaaaatac ccaactgacc gctggcaacc
     5941 agatctttga tcttgttctg aactttagtg aactcttccg gactgttcag atgccaggtc
     6001 gaaacgcctt tcagcatttc ggaggctttg gttgggtcag cttgcagtgc agaagtgatg
     6061 tccacccagt ccagcgccga aagctgatag aaatgaacga tatggtcatg cgtggtgtgc
     6121 gcagccagaa tgatgttacg gatgtattgc gcgttaaccg gaacgtcgat attcagcgca
     6181 ctttctgccg cacgaacgga agacagcgcg tgagtggtag tacatacgcc acagatacgt
     6241 tgcacaatca tccatgcatc gcgcggatcg cggtttttca cgatctcttc catgccgcgc
     6301 cacatggtac cggaagccca tgctttcgaa acgacgccat tttcgatttc gcaatcgatg
     6361 cgtaaatgcc cctcaatacg ggttaccgga tcaatagtaa ttctctggct catgctttgc
     6421 tcgcctcatg acgattatga tcgttttgtt ttaaaggagg aagtatcggc agtagacgaa
     6481 tgagtacgat gtaagcgcaa atctcaatag ccacaaaacc aatagaaatc aacagttctt
     6541 cccaggtcgg gaagtaggcg taaccgccgc ccgggttgaa tgccaccagc gaataggtca
     6601 gacgccaggt tgcacaacct aacagtgcgc tcagtgctga caggaacagc atgcgggaat
     6661 cattacgcag cttcgccaca cgcagaacga ccagcgggaa gagcatcagc aggacttcaa
     6721 tccagaacat cacggagtag aagtcaccgg caaacgctaa cgacagcttg tcgcgataga
     6781 taagctcgcc aaagcgcagc acgatgaaaa tcgccagcaa cacactgatg gtgttggtca
     6841 gcttgacgaa cagactcttt tcatccggac cgttgccacg cagacccgcc tgcaccagcg
     6901 aaccttcaaa gatgacaatc gagaagccca tgatgaacgc cgtcagcagc gagaacagcg
     6961 gcaacatttc atagctctgc cacaacggat gcaccttgta gcccgccgag atcatcagtg
     7021 accccattga agactggtgc atggtcggca acagcgcgcc aagtgcaatg atgaagaaca
     7081 taaccttgtt cagacgcttg agagaaacct tccagcccag acgttcaaac agtgccggag
     7141 caaactccag tgccatcacg ccgatataga tggtcataca gaccgccgtc tcgaacagta
     7201 ccgagttcac gttgaagtga cccggaatgt agaagtacgg caggttccag tagcgaccca
     7261 cgtcgatagt gatcgacaag ccacccagtg agtaaccaaa cagactcgcc aacagcgccg
     7321 gacgcaccag cggatggtat tgcccacggt taaagacgta taccgcccac gccagcgccc
     7381 agccgccaca ggcaaagccg gtgccaatca gcaggtcaaa cgcgatccac acgccccacg
     7441 ggaagccgcc gttcaggtca gagacagagc ccagaccgaa caccagacgc ttcacaatca
     7501 ggagcataca gatgacgatt aacggtccaa aaatcatgac cggtttactg atgattttgc
     7561 cgcccagcgg ttgtggatca tgactcatga tcgtctcctc cgtcgtgatg gtcgtttttg
     7621 gtgttgcgac gaaccagcac ggttaagccc gccagcacag ccagtggtag catcatgcct
     7681 ttatacaggg tgtgttgaat attttcggaa cgcgcaccgg tagaaagatc gtccagtttc
     7741 ggcaggtcga gattttcata aggcacaccc gtcagtacca gaacctgagt accgccgccc
     7801 tctttctcgc cgtacagatg cggataatat ttcggcaccg tatgcaggta agtgtcgcca
     7861 gatttcagcg tctgacgcgg atagtggtat tcgctgccag gcttcagcgc cagacgtttt
     7921 ttcgcctccg ccatcagctc ttcacgcgta ccgaaaatca ccgcgcccgc cgggcacact
     7981 tctacgcagc caggcagacc gcctttatcg agacgttcca cacctttctg gttgcacagc
     8041 tcgcacttat gcagcgcacc aaacgggttg ttgtagtcgt acttcggcac gttgtacgga
     8101 caggcgacca tgcagtaacg gcaaccggtg cacacgtctt tgtcgtaatg gacaatgccg
     8161 gttttcggat cttttttcag tgcagagacc gggcacacag agacacagtt cggatcgacg
     8221 cagtgcatac actgtttctt aatgtacgcg tagccgttct cctcctggtc tttgttgacc
     8281 cctgtgccgc tggtccacac ctgaatgatg ttattggtat acggcgacag tttgtcgttg
     8341 ttcgaccagg tctgttcccc ttgcgggtta cgttcaggga agttgatatc ctgacacttg
     8401 gtgacgcaag cctggcagcc tacgcacaag gtcgagtcgt acaacatccc cagcgatccc
     8461 ggaattggcg ggcggttttc agcagccgca tgactgacag acggcagcgc gcccgtcagc
     8521 aatgccccgc aggaggctgc tttaataaaa ttacgtctgt tcacggttat tctccccgtg
     8581 agtcagcgtt atctttcttt tgctgacgac ccagttcacg caccgccatc acgctgacac
     8641 cggcaaccag cccaaccaca ccgccgagca aaccaatagc gcctgcagag acgttgccgc
     8701 cctctttagc gttaacatcc ggtttctgtg aacgcggagt ctgattttcg acgttggcaa
     8761 gctgatggat gcctttatgg aagccgatac cttcttcgtt acagccatag catgggtgac
     8821 caatcgctac cggccacacg ccgccaacat cgcagaattg cagcgttgag cagttgccgt
     8881 aagtttctgg ccctttacag ccgaggtggt acaggcacca gccttcacgg tggccttcat
     8941 caccgaactc tttggcaaaa cgaccagcat cgaagtgcgg gcgacgttcg cagtgttcgt
     9001 gaatcagacg gccataggcg aaggtcggac ggtttttgtc atccagtttc ggcggtttgc
     9061 cgtaagtgat gatgtgcgca acggtcgcga ggaagttgtg cgggttcggc gggcagcccg
     9121 gaatattgat aacggttttg cctggcagaa cttcttgcag gctgactgcg ccagttgggt
     9181 taactccagc tgcggcaaca ccgccccacg cagagcagga accgatagcg ataatcgctg
     9241 ctgcgccttc tgccgctttg cggatgtgat ccacaatcgg ctcaccagca accatgcaat
     9301 aaataccgtt atcttttaat gggatggaac catccacaac caacacatac tgccctttgt
     9361 acttctcaag tgcgttatgt ttgttctctt cgacctgatg accgaaggcg gcggaaagca
     9421 cttcgtgata ctccagagag atagtctcca gcacgaggtt ttctaccgtt ggatgcgttg
     9481 cacgaagcag agattccgta caaccggtgc actcctgcgc gccaatccag ataactggcg
     9541 gacgctgcgg gttagtaacc gattcggcca tctctgcagc ggctttgcta cttaacccca
     9601 tggtggcggc taatgctgca caaagcttca tgaaatcacg acggttaatg ccgtgagaat
     9661 ggatgagggt gttatctcca gtcatttata gttattccgt tgcgaagacc tggcatatat
     9721 tttgcctcaa tcgcaaaatc aataatgcga tcgatgcgcc atttaccaca catttattat
     9781 ggttatcgat atcatgatac tgcggcgggg cgctaaaacg aagggaagaa gcattagtgt
     9841 aattaattaa agcaagataa tacgtatgtt tgatcaattt tcgaggcgat aaccggccat
     9901 aaaaaagcgc aacgtagaac aggaattatt ctcacgaaag aaatccatta ccatctcttt
     9961 atccagtcca tagcggcgcg tcagaatacc cgcttcccag gcgctaagct ggcgatctcc
    10021 ggtcttttcg tacatccggt gtgagtagcc taatacaaaa ccgcgtttat aatcggcaca
    10081 gaattttgtc acattaaccg cactgtcggc atgcgttgct tttaatcccg ccattaagcc
    10141 tttaccaaaa tggttattca tcgttcacct cattcgctat atatcgccat atatagcgat
    10201 attttaggca aagaacgact tttgcgcacg ctattttttc atgcttgttt aagacgtaat
    10261 ccagtaagag ataaacctat caggagagat atttatcgaa ccatttcagt gtcctttgcc
    10321 aggcaagatc ggcggcagat ttgtcataac ggggcgtgga atcattatgg aatccgtgat
    10381 taacccccgg atagatatac gcctcataaa ccttattatt ggctttcaac gccgcctcgt
    10441 aagcaggcca gccctcgttg attcgggtgt ccagttccgc gaagtggagt agtaaaggcg
    10501 cttcaatctt cgccacatcg gcagtgggtg cctgacgacc ataaaacggc accgcgcagg
    10561 ccagttccgg atacgcgaca gccgccgcgt tcgatacgcc accgccatag caaaatccgg
    10621 taatacccac tttgcctgcc gcttgtggat agcgttgcat aaactcaatt gcggcaaaga
    10681 aatcattcat cagtttggtt ggatcaacct gctgttgcag ctcacgacct ttatcatcat
    10741 ttcccggata acctccaacg gaacttaagc cgtcaggtgc cagggcgata taccccgcct
    10801 tcgccactcg ccgtgccaca tcttcgatat acggattcag tccacgattc tcatgcacca
    10861 ccaccacggc tggcgttttg ccgctcattt ttgcgggttt caccagataa ccccgtacct
    10921 cgccgtgacc atttggcgaa ggatacatga tgtactcagc aacaatctcc gggtcggtaa
    10981 actctacctg agtcgccagc gcataatttg gcttgagcaa atcaaacaac gctaatgccg
    11041 tcatcccgcc caccgcatac ttcgccgcaa ggttgaggaa ctcacgtttc gaaattttcc
    11101 cgtgagcgta atagtcgtag taatccaaca actcttgtgg gaaatctttg gcggttaaac
    11161 gcggcatcgt tgcactcctc agttggtgtt tttttaagca aagcataagc acgtgttttt
    11221 gcccagtttt tcgtcactct gtgagccaga ctacgggata cgcgctggcg aatcgctaaa
    11281 ctagaaacat tgtttcgaaa ttgaacggtg gaaaggagag gtcatggtct ggttagcgaa
    11341 tcccgaacgt tacgggcaga tgcaataccg ctattgcgga aaaagcggtt tacgcctgcc
    11401 cgcgttatcg ctcggtttat ggcacaattt cggtcacgtt aacgcgctgg aatcacagcg
    11461 tgcgatcctg cgtaaagcgt ttgatttggg cattacgcac tttgatttag ccaacaatta
    11521 cgggccgcct ccaggaagcg cagaagagaa ctttggtcgc ctgctgcggg aggattttgc
    11581 cgcttatcgc gatgaactga ttatctctac caaggctggc tacgatatgt ggcccggccc
    11641 ttacggctct ggcggttcac gtaaatacct gctcgccagc ctcgaccaaa gcctgaagcg
    11701 tatagggctt gagtatgtcg atatctttta ctctcatcgc gtcgatgaaa atacgccgat
    11761 ggaagaaacc gcctctgcgc tggctcatgc ggtacaaagc ggtaaggcgc tgtatgtcgg
    11821 gatctcctct tactcgccag agcggacgca aaaaatggtc gagttgctgc acgagtggaa
    11881 aattccgctt ttaattcatc aaccttcgta caatttactg aaccgctggg tggataaaag
    11941 cggcctgctg gataccctgc aaaataacgg cgtgggctgt attgccttta ctcctctggc
    12001 tcagggattg ctgaccggaa aatatctcaa cggcattccg caagattcac ggatgcatcg
    12061 tgaagggaat aaagttcgtg gtctgacgcc gaaaatgctt accgaagcca acctcaacag
    12121 cctgcgctta ttgaatgaaa tggcacagca gcgtggacaa tcaatggcgc aaatggcgtt
    12181 aagctggttg ctgaaagatg atcgcgtgac gtcggtattg attggtgcca gccgcgcgga
    12241 gcaactagag gagaacgtgc aggcgctgaa taatctgaca tttagcaccg aggagctggc
    12301 gcagattgat cagcatatcg ccgatggcga gctgaatctg tggcaggcgt cgtccgataa
    12361 atgacctgtt aataacgggc gcgcgaaccg cgcccgaatc agatcagtga ttatgacgag
    12421 tcagtcggtc aagatagccc atcacaaatg cagaaagcac aaacgtcaga tggataatga
    12481 cgtaccacat cagtttgtta tccgggacat ttttcgcatc cataaagacg cgcagtaagt
    12541 gaatggaaga aattgccaca atcgacgctg ctactttgtt tttcagcgac gttgcgtcca
    12601 ttttccccag ccagttcagc ttctctttgt tctcggagat atccagctgt gagacgaaat
    12661 tctcataacc ggaaaacatc accatcacca gcaaaccgcc aaccagcgtc atatccacca
    12721 gcgacagcaa cacgaggatc aaatctgatt ccgccatcga gaagatattc ggcagtacgt
    12781 gaataatctc ctggaagaac ttcagcgcca gggcaactaa cgccagcgaa aggccaaagt
    12841 acacgggggc aagcagccag cgagaagcat acattgcatt ttcaagaaaa cgttccatac
    12901 attcctgtcg tgtagttaaa acgggcaaca gtatatcgca attgagtaaa cagcacgcaa
    12961 cagcaaatca acatatacgt taaacctttg tcgcataatt ctgaggttta gggatcttcc
    13021 tggttccctt atcaacgcat cgagagcgtc tacccttaaa ggacgttgaa cctgaaggga
    13081 gaaaaacgat gtctcattta aaagacccga ccacgcagta ttacactggt gaatatccca
    13141 aacagaaaca accgacgcca ggcatccagg cgaagatgac accggtaccg gattgcggcg
    13201 agaaaaccta tgttggtagc ggtcgcctga aagatcgtaa agcactggtg acagggggcg
    13261 attccggaat aggtcgcgct gccgccatcg cttacgcgcg tgaaggggct gacgtggcga
    13321 tcagttatct tcccgtggaa gaagaagacg ctcaggatgt gaaaaagatc attgaagaat
    13381 gcggacgcaa agccgttctg ctgccaggcg atttaagcga tgagaaattc gcccgttcgc
    13441 tggttcacga agcgcacaag gcgttaggcg ggctggatat tatggcgctg gtcgccggga
    13501 aacaggttgc cattccggat attgcagacc tcaccagcga acagtttcaa aagacctttg
    13561 ccattaacgt tttcgcgctg ttctggctaa cccaggaagc gatccccctg ctaccgaaag
    13621 gtgcaagtat catcaccact tcgtcaatcc aggcatacca gccaagtccg catttactgg
    13681 actatgcggc tacgaaggcg gcgattctga actacagccg tggcctggca aaacaggtcg
    13741 cggagaaagg tattcgggtg aatattgtcg cgccaggccc tatctggacg gcactgcaaa
    13801 tttccggcgg acaaacgcag gataagatcc cgcagtttgg tcagaaaacg ccgatgaagc
    13861 gtgcggggca accggcggaa ctggcccctg tatatgttta tctggcaagt caggagtcga
    13921 gctacgtcac cgcagaagtg cacggcgtgt gcggcggcga gcatttaggt taaaaaaatg
    13981 cccggttgtg aaaagcaacc gggcatcatt gtgaattcac tcttacctga tgtggcgaaa
    14041 acgccttatc cggcctacaa acgcacgaag attcaacatg ttgcaataga ttttgtaggc
    14101 ctgatgagcg tagcgcatca ggcaattcta ctttacttcg ctttggcggt ttcttcgccg
    14161 accagaccta tcttcaggta acccgcctga tgcagcgtat ccattacctt catcaacgtc
    14221 tcgtaatcga cggttttgtc cgctcggaag aagatggtgg tgtctttctt gccttcggtt
    14281 aacgcattca acgccgtaat cattgtttca tcggtgaccg gatcgttacc gataaacatc
    14341 gagttgtctg ccttcaccga cagataaacc ggtttttccg ggcgcggctg cggcgtgctg
    14401 gtagaagcag gcaagttcac cttcacatct accgtcgcta acggtgccgc caccataaag
    14461 ataatcagca gaaccaacat cacgtcgata aacggcgtca cgttgatatc atgcatttcg
    14521 ccgttatcgt cgaggttttc gttaagatgc attgccatcg gacattatcc tgcgcgtaat
    14581 ttttgtgcga cacgaaccgg atgcgcagcg gcgctggctt ccagatccag gtcacggctt
    14641 tgcagcaaca atacctgcgc tgcaacatca cccagcatcg ctttaaagcc gccaatctgg
    14701 cgtgcaaata cgttatagat aacgaccgca ggaatcgctg ccacgaggcc gattgccgtt
    14761 gctaacagag cttctgcgat acccggcgca acgactgcca ggttagtggt ctgcgtttgc
    14821 gcgataccaa taaagctgtt catgatgccc cagaccgtac caaacagacc aacgaacgga
    14881 gaaatcgcgc cgatggttgc cagatagccg ttaccgcggc ccatttgacg acccactgcg
    14941 gcgacccgac gctccagacg gaagctggta cgttctttaa taccttcgtt atcgtcgctg
    15001 ccttctgaca gttccagctc attctgcgct tcattgagca aatgcaggct taagcttttg
    15061 ctaccaaaat cagcggcgat atcgttggcc tggtttaagg aacgcgcttc agccagcagt
    15121 tgctgctcgc gcttaaggcg acgcttctga ttgaagaact ctacgctctt actgaagaag
    15181 attgcccaag tgactacgga ggccaaaata agcccaatca tcacgcactt aacgacaata
    15241 tcggcgtgct gatacatacc ccagacggaa aggtccgtct gcatcaaatt attacccact
    15301 gtgtatctcc aggacgcaag tcacaaaatc tgcgcataat aatatcaaaa tgacgtcgaa
    15361 ttgatagtcg ttctcattac tatttgcata ctgccgtacc tttgctttct tttccttgcg
    15421 tttacgcagt aaaaaagtca ccagcacgcc atttgcgaaa attttctgct ttatgccaat
    15481 tcttcaggat gcgcccgcga atattcatgc tagtttagac atccagacgt ataaaaacag
    15541 gaatcccgag atggcggaca aaaagcttga tactcaactg gtgaatgcag gacgcagcaa
    15601 aaaatacact ctcggcgcgg taaatagcgt gattcagcgc gcttcttcgc tggtctttga
    15661 ctgtgtggaa gccaaaaagc acgcgacgcg caatcgcgcc aatggcgagt tgttctatgg
    15721 acggcgcgga acgttaaccc atttctcctt acaacaagcg atgtgtgaac tggaaggtgg
    15781 cgcaggctgc gtgctatttc cctgcggggc ggcagcggtt gctaattcca ttcttgcttt
    15841 tgtcgaacag ggcgatcatg tgttgatgac caacaccgcc tatgaaccga gtcaggattt
    15901 ctgtagcaaa atcctcagca aactgggcgt aacgacatcg tggtttgatc cgctgattgg
    15961 tgccgatatc gttaagcatc tgcagccaaa cactaaaatc gtgtttctgg aatcgccagg
    16021 ctccatcacc atggaagtcc acgacgttcc ggcgattgtt gccgccgtac gcagtgtggt
    16081 gccggatgcc atcattatga tcgacaacac ctgggcagcc ggtgtgctgt ttaaggcgct
    16141 ggattttggc atcgatgttt ctattcaagc cgccaccaaa tatctggttg ggcattcaga
    16201 tgcgatgatt ggcactgccg tgtgcaatgc ccgttgctgg gagcagctac gggaaaacgc
    16261 ctatctgatg ggccagatgg tcgatgccga taccgcctat ataaccagcc gtggcctgcg
    16321 cacattaggt gtgcgtttgc gtcaacatca tgaaagcagt ctgaaagtgg ctgaatggct
    16381 ggcagaacat ccgcaagtag cgcgagttaa ccaccctgct ctgcctggca gtaaaggcca
    16441 cgaattctgg aaacgagact ttacaggcag cagcgggcta ttttcctttg tgcttaagaa
    16501 aaaactcagt aatgaagagc tggcgaacta tctggataac ttcagtttat tcagcatggc
    16561 ctactcgtgg ggcgggtatg aatcgttgat cctggcgaat caaccagaac atatcgccgc
    16621 cattcgccca caaggcgaga tcgattttag cgggaccttg attcgcctgc atattggtct
    16681 ggaagatgtc gacgatctga ttgccgatct ggacgccggt tttgcgcgaa ttgtataaca
    16741 ttgccacttt tggacaattt tgcagacatt taattgtgaa aagtcttaaa ttgttgcgtc
    16801 cgggatcaag gcgtcccgga cgaatcagga gtacaatagg cagataaagg cttaaacgct
    16861 gttccacagg aaagtccatg gctgttattc aagatatcat cgctgcgctc tggcaacacg
    16921 actttgccgc gctggcggat cctcatattg ttagcgttgt ttactttgtc atgtttgcca
    16981 cgctgttttt agaaaacggc ctgctgcccg cctcattttt gccaggcgac agcttgttga
    17041 tactggcagg cgcattgatt gcccaagggg ttatggattt tctgcctacg attgcgattc
    17101 tgaccgccgc agcaagtctg ggctgctggc taagttatat tcaggggcgc tggttaggga
    17161 ataccaaaac ggtgaaaggc tggctggcac agcttcctgc taaatatcac cagcgcgcca
    17221 cctgcatgtt tgaccgccac ggtctgctgg cgctgctggc tggacgtttt cttgcatttg
    17281 tccgtacgct gctgccaacc atggcgggaa tttccggtct gccaaaccgc cgcttccagt
    17341 ttttcaactg gttaagtgga ttgctgtggg tcagcgtggt aaccagtttt ggctatgcct
    17401 taagtatgat tccgttcgtt aaacgccatg aagatcaggt aatgacgttc ctgatgatcc
    17461 tgccaattgc cttgttaacc gctggcttgt taggcacgct gtttgtagtg attaaaaaaa
    17521 aatactgtaa cgcctgacga ttttccccgt tcccggctgc tgtaccggga acgtatttaa
    17581 ttcccctgca tcgcccgcat tcttgccgca tcttcccccg gcgtcacacc gaagtaacgt
    17641 ttaaactcac ggctaaattg cgatgcgctt tcatagtcga cgcgcatcgc tgctgcgctg
    17701 gccttcatgc cgtcatggat gatcatcatc cgcgccttat gcagacggta attcttcaaa
    17761 tactgcaacg gcgaggtgct ggtgacagac ttaaaattat gatggaacgc cgatacgctc
    17821 atgttggctt ctgccgccag ttgctcgacg ctcaggtttt cggtgtattt attctcaatc
    17881 cgtttcagca cgcggctaat cagactgaag tgagtctggc gactgaccag cgccagtaac
    17941 gcgccgccgc aaggtccggt cagcacgtag tacagaattt cgcggatgat ctgtttgccg
    18001 agaatacgcg catccagtgg tcgctccatc acgtcgagta accgctccgc cgcgcataaa
    18061 atctcttctg ataacgtggc ggagttaatc ccgctggctg ccatcgacgg ctggaaatgc
    18121 tcatcttcgc caatgtccat caacagttcc tgtaactgca aaatatcgac attgagacgc
    18181 aatcctgcca gcggcacctc tgacgtggca taggtttcgc actcaaacgg caacggcacc
    18241 gtcagcagca ggtattcatt ggcatcataa cgaaacacgc gttcattgat ataaccgatt
    18301 ttatgcccgg aaaagagaat tatgatgcca ggctcgtaca tcaccggtgt acgtgcgaaa
    18361 ggcgtctcgc catacaacaa acgcacatcg ggcaacagtt ctgacaaact attttcttta
    18421 tttttcagtt tattaacttt atccgccagc aagcggcaaa tctcttcacg tttcatatcg
    18481 cgtaatttct taggaataat gcggcaattt gattgtgcgc aattttgtag catttctcca
    18541 gcactctgga gaaataggca agacattggc agaaatgagc attgagagcc agggcgctgg
    18601 cgatcacaat gaaaaacatc aggcagatcg ttctctgccc tcatattggc ccagcaaagg
    18661 gagcaagtaa tgaacaactt taatctgcac accccaaccc gcattctgtt tggtaaaggc
    18721 gcaatcgctg gtttacgcga acaaattcct cacgatgctc gcgtattgat tacctacggc
    18781 ggcggcagcg tgaaaaaaat cggcgttctc gatcaagttc tggatgccct gaaaggcatg
    18841 gacgtgctgg aatttggcgg tattgagcca aacccggctt atgaaacgct gatgaacgcc
    18901 gtgaaactgg ttcgcgaaca gaaagtgact ttcctgctgg cggttggcgg cggttctgta
    18961 ctggacggca ccaaatttat cgccgcagcg gctaactatc cggaaaatat cgatccgtgg
    19021 cacattctgc aaacgggcgg taaagagatt aaaagcgcca tcccgatggg ctgtgtgctg
    19081 acgctgccag caaccggttc agaatccaac gcaggcgcgg tgatctcccg taaaaccaca
    19141 ggcgacaagc aggcgttcca ttctgcccat gttcagccgg tatttgccgt gctcgatccg
    19201 gtttatacct acaccctgcc gccgcgtcag gtggctaacg acgtagtgga cgcctttgta
    19261 cacaccgtgg aacagtatgt taccaaaccg gttgatgcca aaattcagga ccgtttcgca
    19321 gaaggcattt tgctgacgct aatcgaagat ggtccgaaag ccctgaaaga gccagaaaac
    19381 tacgatgtgc gcgccaacgt catgtgggcg gcgactcagg cgctgaacgg tttgattggc
    19441 gctggcgtac cgcaggactg ggcaacgcat atgctgggcc acgaactgac tgcgatgcac
    19501 ggtctggatc acgcgcaaac actggctatc gtcctgcctg cactgtggaa tgaaaaacgc
    19561 gagaccaagc gcgctaagct gctgcaatat gctgaacgcg tctggaacat cactgaaggt
    19621 tccgacgatg agcgtattga cgccgcgatt gccgcaaccc gcaatttctt tgagcaatta
    19681 ggcgtgccga cccacctctc cgactacggt ctggacggca gctccatccc ggctttgctg
    19741 aaaaaactgg aagagcacgg catgacccaa ctgggcgaaa atcatgacat tacgttggat
    19801 gtcagccgcc gtatatacga agccgcccgc taagcttttt acgcctcaaa ctttcgtttt
    19861 cgggcatttc gtccagactt aagttcacaa cacctcaccg gagcctgctc cggtgagttc
    19921 atataaagga ggaacgtatg gctaatccaa ccgttattaa gctacaggat ggcaatgtca
    19981 tgccccagct gggactgggc gtctggcaag caagtaatga ggaagtaatc accgccattc
    20041 aaaaagcgtt agaagtgggt tatcgctcga ttgataccgc cgcggcctac aagaacgaag
    20101 aaggtgtcgg caaagccctg aaaaatgcct cagtcaacag agaagaactg ttcatcacca
    20161 ctaagctgtg gaacgacgac cacaagcgcc cccgcgaagc cctgctcgac agcctgaaaa
    20221 aactccagct tgattatatc gacctctact taatgcactg gcccgttccc gctatcgacc
    20281 attatgtcga agcatggaaa ggcatgatcg aattgcaaaa agagggatta atcaaaagca
    20341 tcggcgtgtg caacttccag atccatcacc tgcaacgcct gattgatgaa actggcgtga
    20401 cgcctgtgat aaaccagatc gaacttcatc cgctgatgca acaacgccag ctacacgcct
    20461 ggaacgcgac acacaaaatc cagaccgaat cctggagccc attagcgcaa ggagggaaag
    20521 gcgttttcga tcagaaagtc attcgcgatc tggcagataa atacggcaaa gccccggcgc
    20581 agattgttat ccgctggcat ctggatagcg gcctggtggt gatcccgaaa tcggtcacac
    20641 cttcacgtat tgccgaaaac tttgatgtct gggatttccg tctcgacaaa gacgaactcg
    20701 gcgaaattgc aaaactcgat cagggcaagc gtctcggtcc cgatcctgac cagttcggcg
    20761 gctaacatgc aaattctccc ggtgacggta atgttccgct accggacttt tcagaaatca
    20821 tttattcccc tcgcgtcccg cccgttgtta ctcttccttg ttcaggaatg ccaaatataa
    20881 ggacatcatc atgcagagcc ggaagctctt aaaagaacaa ctcatctata tccgggataa
    20941 acgcaacgga gaggtgaaaa acagatgaaa ataatacttc tgtttttagc agccctggca
    21001 agttttaccg tacacgcgca gcctccttca cagaccgtag aacaaacagt ccggcagatt
    21061 tatcagaact ataaatcaga tgccagtaca ccttatttcg gtgaaaccgg agagcgggcg
    21121 ataacttcag cgcgtattca acaggcgctt accctgaacg acaatcttac gctgccgggc
    21181 aatattggct ggctggatta tgatccggtt tgtgattgtc aggattttgg cgatctggtg
    21241 ctagaaagcg ttgcgataac ccaaactgac gccgatcatg ccgatgccgt tgtgcgcttt
    21301 cgtatcttta aagatgataa agaaaagacc acgcagacac tgaaaatggt ggcggaaaat
    21361 ggtcgttggg tcattgacga tattgtcagc aatcatggca gcgtcttaca agcagttaat
    21421 agcgagaatg aaaaaacgct ggccgcttta gcttcgttgc aaaaagaaca gccggaagcc
    21481 tttgttgccg aactctttga acatattgct gattatagct ggccgtggac gtgggtggtt
    21541 tccgactctt accgccaggc ggttaatgcc ttctataaaa ccaccttcaa gacggccaat
    21601 aatcccgatg aagatatgca aatagaacgg caatttattt acgacaatcc gatctgtttt
    21661 ggcgaagagt cgctattttc acgcgttgat gaaattcgag tcctggagaa aaccgccgat
    21721 tccgcccgca ttcatgttcg ttttacgctg accaatggca acaacgaaga gcaagaactg
    21781 gttttacagc ggcgcgaagg caagtgggaa atcgctgatt ttatccgccc gaacagcggc
    21841 agcctactta agcagattga ggcaaaaacc gccgccagat taaagcaatg agctgaatta
    21901 aataacaatt agccggaaca ataaataaaa gggaacacta tatgaaaacg attttcaccg
    21961 tgggagctgt tgttctggca acctgcttgc tcagtggctg cgtcaatgag caaaaggtca
    22021 atcaactggc gagcaatgta caaacattaa atgccaaaat cgcccggctt gagcaggata
    22081 taaaagcact acgcccacaa atctatgctg ccaaatccga agctaacaga gccaatacgc
    22141 gtcttgatgc tcaggactat tttgattgcc tgcgctgctt gcgtatgtac gcagaatgat
    22201 aaaaaaatcc ccggcagcat gtcagttgcc ggggattttt tttaacgtcc aaccgccgct
    22261 ttagggcgtt tcttcgcacc agcattcacc ggacgagatt gcgtagacga cgcttttttt
    22321 gccgtagcag gcgtctgacg ctgggtcgcc atcggcgtat gtttagtcaa cgccggacgg
    22381 gtattgcggt tctggcgacg agcttcacgc atctcttcaa tggttggcgc aggcactaag
    22441 caatcgcgac ggctgccaat cagatgcttt ttgcccatcg cttccagcgc cgggcggatt
    22501 aacggccagt ttgccggatc gtggtaacgc aacaacgctt tatgcaaacg acgctgtttg
    22561 tcgcccttcg gtacgaagac gtcttcgctc ttataaccaa tcttcgccag cggttttttc
    22621 ccggtgtaat acatggtggt tgagttcgcc agcggcgatg ggtagaagtt ctgtacctgg
    22681 tcgagacgga aacgatgctt tttcagccac agcgccaaat tcaccatatc ttcatcacgc
    22741 gtaccggggt gcgcagagat gaaatacggg atcagatact gctctttacc tgcctgtttc
    22801 gagtaggtat cgaacagctc tttaaagcgg tcatagctgc ccatgcccgg cttcatcatc
    22861 ttcgacaacg gcccttcttc ggtatgttcc ggagcaatct tcagataacc gccgacgtga
    22921 tgggtcgcca actctttgat atagcgcgga tcttccacgg cgatgtcata acgtacgcct
    22981 gaggcgatga ggattttttt aatgcctttc agatcacgcg cacggcgata gaggttgatc
    23041 gtcggttcgt ggttagtgtc catgtgcgga caaatatccg gataaacgca cgacaaacgg
    23101 cgacaagttt gttcagcgcg tggcgatttg cagtgcaaca tatacatgtt ggcagttggc
    23161 ccaccgagat cggaaatcac gcccgtaaaa cctggaacgg tgtcgcggat cgcttcgatc
    23221 tcattaatga tcgaatcttc cgaacggctc tgaataatgc gcccttcgtg ctcggtaata
    23281 gaacagaaag aacagccgcc aaagcagccg cgcataatgt tgaccgaaaa acggatcatt
    23341 tcgtaagccg gaatacgggc attgccatag gccggatgtg gcacgcgctt gtacggcagc
    23401 gcaaaaacgc tgtccatctc ttcggtagaa agcgggatag caggcgggtt gatccacaca
    23461 tagcggtcac cgtgtttttg catcaatgcg cggacgcagc ctgggttggt ttcgtggtgc
    23521 agaatacgcg aagcatgggc gtacagcact ttatcgccct tcactttctc gaaagaaggc
    23581 agcaacacgt aggttttttc ccacggtttc gggcgcggtg gctgcacggt tacggctttg
    23641 gcttcctgct ttttcggtgc caccggtttg ttatccgcgc acggcaaatc ttcaccatac
    23701 ggatgcggga ttgggtcgat ttttccaggg gtatcaagac ggggggaatc cacgccgctc
    23761 cagccaggca gcgcctcttt cacgataatc gcggtattac gcacatcgcg gatttcacta
    23821 atcggctcgc ccatcgccag acggtgcgcc acctccacca gcggacgctc accgttacca
    23881 aacatcagca tgtcggcttt cgaatccacc agcacggaac ggcgcacggt atcggaccag
    23941 taatcataat gtgcggtacg gcgcagacta gcctcaatac cgccgaggat caccggtaca
    24001 tctttccacg cctctttaca acgctgggta taaaccagtg tggcgcgatc cgggcgctta
    24061 cccgcgacgt tatccggcgt gtaggcatcg tcatgacgta aacggcgatc ggcggtataa
    24121 cggttgatca tcgaatccat gttgccagca gtaacaccga aaaacagatt cggtttaccc
    24181 agacgcataa agtcgtcttt gctgctccag tccggctggg cgatgatccc gacgcgaaag
    24241 ccctgtgctt ccagcatacg accgcaaatc gccatcccga agcttgggtg atcgacatac
    24301 gcgtcgccag taaccaaaat gatgtcgcag ctatcccagc caagttgatc catctcttca
    24361 cgagacatcg gcaaaaacgg tgccggtcca aaacaggcgg cccagtactg cggccaggag
    24421 aacaggtcgc gatccggttg gatcagggag atagagctca ttttgcttcc agaaatgata
    24481 aaaaaataat caaaggccgg ggattataag ccggaacgaa agagaaatcg aaaagtattc
    24541 catactcgcc ctcctcgggc gagtatgaag attacggtac cggattgact aacagttgcc
    24601 caatcgagcc acggtccgcc atttccagcg tctgactgtt gaagtagaac gggaagtgcg
    24661 cccaggaagg ctgaccgaaa taaacaagca gctccacttg tccgtcaacc caaacggtat
    24721 ctttccagcc tctgtcttcc gggaacggca tcgcaccatt cacgttacgg atctggaaca
    24781 tcacgccttc aatatggaac gcttgcggct cgtccgcgcg taccgtccag cgttcccacg
    24841 ttccttgctg cgcggtgaca tcaatacggt tgacgtccca cagctgtcca ttaatacccg
    24901 ggtcatcacc cagactgata tcgcgactgc gaatcggcga accggccatg atttcagttg
    24961 gcagcaagcg catcggaaga ctgtctgtga ccagcggcag aaggccggtt gggcgcagcg
    25021 ttagcaccag ggtagaaacc agaatactgg atggctcaaa gaagccgcga atacgatcaa
    25081 caatgctcgc cgcttcgcca caggtgatcg acacttcatc gccgttgctc atatccacca
    25141 gaatctcgcg gcgctcgccc ggtgccagcg aaagttgctt caccgataca ggagcaggca
    25201 ggaatccctg atcgccagaa atcacatgta acgggcgacc atcgctcatc tgtagttgat
    25261 agcgacgaga gttcgacgcg ttcagcagtc gcaagcgcac ccagccacgc gagacttcaa
    25321 cgtacgggct ttgtacaccg ttaaccagca gcgtatcacc aacaaagccg ccgcttcccg
    25381 gttcgttgta ttctggcgta ccaaagttat ccagccgttt atcctggata atgaccggaa
    25441 aatcatccac atcataatgg ttggggatag gcagcgactt gctgacttca tcttccacca
    25501 gccacattcc ggcaaggccg ttatagacct gctgagccgt gcggttggga gtattggcgt
    25561 gataccacag agtagctgcg ttctggcgaa taggcagtac gggtgcccag tcagcgtttg
    25621 gcgacatcat ccgtgccgga ccgcccatca gcgggccagg tacctgtagc ccggccaccg
    25681 tcattgagac attttctgtc aggcggttgc tgtaaataag cttaacatcg tcgcccttcc
    25741 agacgcggat agtcggcccc aggtaacgac cattgattcc ccagaccgac gcgcgtgtcc
    25801 ctggcgtaaa tgaccagtgc gcacgttgta cagtcataaa cagcggttgc ccacggcgag
    25861 attcaagtag cggcggaacg ggtagcggtt gctgttgccc ggctgcgctg gccttcaggg
    25921 gaacagcgcc tgcacaaagt gcaatccccg atgcctgaat gaactgacgc cgactgagtg
    25981 acatatttgc tccatgtaaa actggctaaa gtaataccgt gcaggaaagt gttccccgca
    26041 aaaatacaga ttgccgactt aaacttttcc ggcggcttcg cgttctgcga cttctttatc
    26101 gagctcggcg attttttgtt ccattatcga acgacaatgg gcagccagct cacgaacctg
    26161 atctttgcca tactgactga cgtcaattgg cggcagcatt tcgacaatca ccagaccgtt
    26221 gtgcagtcga ttaagattaa tcttattcga agttgtagag acgcacacgg gaataatcgg
    26281 gacgcccgcc gcaattgccg cgtgaaatgc tccagtcttg aacggtagca ggccgcgacc
    26341 acggctgcgg gttccttccg ggaacatcca gatggaaata cggcgttttt tgaaatgatt
    26401 cacgacttcc gcaatggtgc cgtgagcttt agtgcgattg tttctgtcga tcaataagtt
    26461 gccggttaac cagtacaact gcccgaagaa ggggatccac agcaagctct ttttacctac
    26521 cgtcaccgtc ggcggttgca caatgttcga tgctgtcacc atgtcatagt tgttctggtg
    26581 gttagcgata tagatagcat tgccgtagct ttcagcatct gcaggtttgc ggcactcaac
    26641 tttcaggccg aacagcggcg caagacggcc aaacatatgc ccaaaggtgg ccacatgttt
    26701 cgggttacgc gggctgaaaa ggcagtaaat ggagccgaat acacagacta agatgctgta
    26761 aatcacggta ataataagac gaaagatata tagcataaca ccctctgaag gtctgagaga
    26821 ctggcattgt acgtcacctg aaatcaggtt agggcattgt taaaagccgc tctctggaaa
    26881 agcgtattgt taatcgcaac gcatgaataa acaacggttt caagggaatt tttatcgttt
    26941 ccgggggttg aatacgctgc cggatgacga cttaacgttt catccggcgt tccttgcaag
    27001 cgggaggaaa caggccctcc ccggcatatt actcttcgct atcaccgttg ctggcacggc
    27061 gaggagagtc gatctcaaca cgatcgatac gctgcaaacc gcgcatcaac gtaccgcggc
    27121 gtccacgttc accagtgact ttctgtaact cttccgggcg cagtttaatt ttgcgtttcc
    27181 caacatgaat ggtcagcgtg ctttgcggcg gcagaacgta caattgcgcc agaccatctt
    27241 ctccacgcgc ggcttctgcc gatggaatgt tgataatctt gttgcctttg cccttcgaca
    27301 gctgcggcag atcgcttacc gggaacatca acatacggcc tgcctgagtg attgccagca
    27361 gcatatcgga agcatcttca atcaccaccg gcggcataac atgggcattt tccggtaagg
    27421 tgatcaaagc cttacctgca cggttacgcg ccaccagatc gttaaaggtg cagacgaaac
    27481 cgtaacccgc atcggaagcc atcagcagtt tctgatcgtc gctttccatc agcatatggt
    27541 caacggtcgc cccaggcggc aacgttaatt tgccggtgag tggttcgccc tgaccacgcg
    27601 ccgacggcag cgtaatcggg tcgatggcat agctacgacc ggtggaatca acaaacacta
    27661 ccgattggtt gctcttacct ttcaccgcca ctttgaagct atcacccgct ttataattca
    27721 ggcccggcgc gtcgatatca tggcctttag cgctgcgtac ccagcccatc tgcgacagca
    27781 caatggtgac aggttcagac ggcagcatgt cgtgctcgct catcgctttc gcttcttcgc
    27841 gttcctgcaa cggcgaacga cgatcgtcac cgtaggcttg cgcgtctgcc tgcagttctt
    27901 tcttcagcag gttattcatt ttacgctcgg aagccaaaat gccctgcaac tggtcgcgct
    27961 ctttttccag ctcactctgc tcaccgcgaa tcttcatctc ttccagtttg gcaagatgac
    28021 gcagtttcag ttcgaggatc gcttccgcct gggtttccgt aaggccaaac cgcgacatca
    28081 gcgccggttt cggttcatct tcgttacgaa tgatctcaat cacttcgtcg atgttgagaa
    28141 acgccaccag caaaccttcc aggatatgca ggcgcttgag gactttctcc agacgatagt
    28201 tcagtcggcg gcgcacggta tcgcgacgga acaccagcca ttcggagagg atttccagca
    28261 ggtttttcac cgccggacga ccatccagac cgatcatatt gaggttaatg cgatagctct
    28321 tttccagatc ggtggtagcg aagaggtggt tcatcacctg atccatatcc acgcggttgg
    28381 aacgcggcac aatcaccaga cgggtcgggt tctcgtggtc agattcatcg cgcagatcgt
    28441 caaccatcgg cagctttttg ttgcgcattt gcgcagcaat ttgctccagt acacgcgcac
    28501 ctgaaacctg atgcggtaat gcgctgataa ccaccgcgcc atcttctttc ttccacaccg
    28561 cgcgcatacg tactgaacca cgtccgttct cgtagatttt acggatctcg gcgcgcgaag
    28621 tgataatttc cgcttcagtc ggataatccg gcccctgcac gatatccagc agctgatcga
    28681 gcgtggtttt cggctggtcg attaatgcga ttgccgcctg agctacttca cgcaggttat
    28741 gcggtggaat atcggtcgcc atgccgacgg caataccggt ggtgccgtta agcaaaatgt
    28801 ttggcagacg agcaggcagc attttcggct cctgcaaagt gccgtcgaag tttggcaccc
    28861 agtcagccgt cccctgcccc agctcgctca atagcagctc ggaatatttc gacaaccggg
    28921 attcggtgta acgcattgcc gcgaacgatt tcggatcgtc cggcgcgccc cagttcccct
    28981 gaccatcaac cagcggataa cggtaagaga acggctgcgc catcaggacc atcgcttcat
    29041 aacaggcgct atcgccgtgc ggatggtatt tacccagtac gtcaccgacg gtacgggccg
    29101 attttttaaa tttggcgctg gcattcaggc ccagttcaga catcgcatac acaatgcggc
    29161 gctgaacagg tttcagacca tcaccaataa acggcaacgc acggtccatg atcacgtaca
    29221 tggagtagtt taagtaggcg ttttccgtaa attcatgtag cgcaaggcgc tctgccatat
    29281 cgctcattaa ttctgattcc tcaactgatt cgccagcctt caaaggggca atattgccgg
    29341 agatactaca ttatctgccg cgtcgagtca caggagatca gcgtgaatag cgccgccgga
    29401 cgtgctccga cggcgataaa ggcatcaatg tgccttgata tacaacgctt tgctgtagac
    29461 ttgatcggca tagagggcag cacaaggttg cgcccatgcc acaccactcg gcatgggggt
    29521 ccgctccggg cgattcgaga agttgaggtt atgcgagtcc agggaagcct aaagcatcaa
    29581 agtagtcgac tataagtata tggttcagcc cagtttagct gtgtctccac cagtgccccg
    29641 cgcctcctgc cataagtgcg gcttgctcat gggtaccccc gagacggctt acggcgcacc
    29701 ggataataaa tttcgcgtat acatggtatt ccttaccgga gaaattataa gcccaacaac
    29761 aatacgcctg tggcattgtt cgctcaaact tatcgcgagt gatttggctc acactccggt
    29821 aaagattccc catcacgcta acggcacata aacatccgtt tccagtcgct gctccgtcac
    29881 cccttcggca agattggtgt agtgaaacag aatcggtgct ttacgcattt tctcgccgct
    29941 tgcaggcagc cagtggcgaa taatgcccca tatcgtgtgt gaaatatcgt ccagctcgcc
    30001 aacgtggcgg gccacggcat aacgtccacc ggtaagttca ccattgctca caccataacg
    30061 attatcggga atcggttcgc taacgctgcc gcagatatca aagcgaaacg cttccggtgc
    30121 ggtggtggca gggtcgtccc aggcgacgcc aaaagtctgg ctttggttaa caggagaaag
    30181 ccccgtctct ttacgccaca tgataaattt cgccacactg tagttgagca actcagggct
    30241 acagcggtgc ggcaacatcg ccacagggat tgatgggaaa tcgataatgt ttacatccag
    30301 tgtcaggttt gtcattttgc ctccctgcga aattattccc gggaacgcgt cccgggagcg
    30361 gtaacaatta cggatttact ttgcggatct gtttgacgtc aatttcaaca gaattccagt
    30421 ctttatcgac ttcaccctga atctcaaccg tatctttcgg cgtcaccgtc acaccgttcc
    30481 agcgtttgtg gtcgatatca acattgatag taccgctggc atctttaaac acgtagagat
    30541 cgtcagagat gcgttcaacg atattgccgc gcagggttac ccaggtgtcg tcacgcaggg
    30601 attttgcgct ttctacagtc gttacgctgc cgttcggccc ctggaatcct ccggcctgac
    30661 tttgcgttgc cgatgggcca gaaaaaccgc cctgctctgc tgccatcacc ggtgcactgc
    30721 acagggccat tactgcgatt actgctgcga attttttcat gtttattact ccctttaatg
    30781 tctgtttccg agcatttaac aagatagtcc ttaacaactt cttaagggaa aaaaataaaa
    30841 tttaatgctg tacagagcgc gttacaacac ggtttactgg cagcaaatac ggttatcgca
    30901 gggatgaaaa aatgcgaatt ttactgatag aagatgacat gctgattggc gacggcatca
    30961 aaactggcct tagtaaaatg ggttttagtg tcgactggtt tacacaaggt cgtcagggaa
    31021 aagaggcgct ttatagcgca ccttatgatg cggtgatcct ggatttaacc ttaccaggca
    31081 tggatggtcg cgatattttg cgcgaatggc gagagaaagg tcagcgtgag ccggtactga
    31141 tcctgaccgc gcgtgatgcg ttagcggaac gtgtagaagg gctgcgtctg ggagctgacg
    31201 attatctgtg taaacctttt gcgttgatag aagtcgccgc caggctggag gctctgatgc
    31261 gccgaaccaa cggccaggcc agcaacgagc tgcgccacgg taacgttatg ctcgaccccg
    31321 gcaaacgtat cgccacgctg gctggcgaac ccttaacact gaaaccaaaa gaatttgccc
    31381 tgctggaatt actgatgcgt aacgctggtc gggtactgcc gcgcaaactg attgaagaga
    31441 aactgtatac ctgggacgaa gaggtcacca gtaatgccgt tgaagtgcat gtgcatcatc
    31501 tgcgacgcaa actcggtagt gattttattc gtaccgtgca tggtattggc tacacattag
    31561 gtgagaaatg aaatttaccc aacgtcttag tctgcgcgtc aggctgacgc taatcttttt
    31621 aattctggcc tcggtgacct ggctgctttc cagctttgtc gcctggaaac aaacaacgga
    31681 taacgtcgat gaattgttcg acacccaact gatgctgttt gccaagcggt taagtacgct
    31741 cgatctcaac gaaatcaacg cggcggatcg catggcacag acgccaaata aattaaaaca
    31801 cggtcatgtt gatgacgatg cgctgacctt tgccatcttt acccacgacg gcagaatggt
    31861 ccttaatgat ggcgataacg gagaagatat tccctatagc tatcaacggg aaggttttgc
    31921 tgacgggcaa ctggtcggtg aagacgatcc ttggcgtttt gtctggatga cctcacctga
    31981 tggcaaatat cgcatcgttg ttggccagga atgggaatac cgtgaagaca tggcgctggc
    32041 gattgttgcc gggcaattga tcccgtggct ggtcgcactg ccgattatgt taatcatcat
    32101 gatggtacta ctgggtcgtg aactcgcgcc gctgaacaaa ctggcgctgg cactacgtat
    32161 gcgtgaccct gactcggaaa aaccactaaa cgcgactggc gtacccagcg aagtgcgacc
    32221 actggttgag tcgctaaatc aactgttcgc ccgcacacat gcgatgatgg ttcgagaacg
    32281 acgctttacc tccgatgcag ctcacgaact acgtagcccg ttaacagcgc tgaaagtgca
    32341 aaccgaagtt gcgcagctct ctgacgatga tccgcaggcg cggaaaaaag cactgctcca
    32401 attacattcc gggatcgatc gcgctactcg tctggttgat caactgctca cgctatcgcg
    32461 gctggactca ctggataacc ttcaggacgt cgcggagatc ccgcttgaag atctcctgca
    32521 atcgtcggtg atggatattt accacacggc gcagcaggcg aaaattgacg tgcgactgac
    32581 actcaatgcc cacagcatca aacgcactgg acaaccgcta ttgctaagtt tgttggtgcg
    32641 aaaccttctg gataacgcag tgcgctacag tccacagggc agcgtggtgg acgtcacgct
    32701 gaatgccgat aatttcatcg tgagggataa cggccccggt gtgacaccag aggcactggc
    32761 gcgaattggc gaacgcttct atcgcccccc cggacaaacc gctaccggca gtggacttgg
    32821 gctatcgatt gtccagcgaa tcgccaaatt gcatggcatg aatgttgaat ttgggaatgc
    32881 ggaacaaggt ggatttgagg cgaaggtgag ctggtaaggg taagactttt gctaaattcg
    32941 caaaagactt tgcacatttt gctaatttta ccgtaccgct ctgtgacgta ctatagtcga
    33001 caaacgtctc accttgaggt taaaaaatga gcaacaccct gattatcaac ggcgcgaaaa
    33061 aattcgccca ctccaatggt caactgaacg acaccctgac cgaagtcgcg gatggcacac
    33121 tgcgcgacct cgggcatgat gttcgcatcg ttcgcgccga cagcgactac gatgtcaaag
    33181 cggaagtaca aaactttctc tgggctgatg tggtgatctg gcagatgcca ggctggtgga
    33241 tgggcgcgcc gtggacagtg aaaaaataca ttgatgatgt attcaccgaa ggtcacggga
    33301 cgctgtatgc cagcgatggc cgtacccgca aagatccgtc aaaaaaatac ggttccggcg
    33361 gcctggtaca gggcaaaaaa tatatgcttt ctctgacctg gaacgcacca atggaagcct
    33421 tcaccgaaaa agatcaattc ttccacggcg ttggcgtcga cggtgtgtat ctgccgttcc
    33481 ataaagcaaa ccaattcctt ggcatggaac cgctgccgac atttatcgct aatgacgtga
    33541 taaaaatgcc tgatgttccc cgctatactg aagaatatcg caagcatctt gtggaaattt
    33601 ttggttaact agagctcagg ctttagaagg agttaaccat gcttaccgta atcgcagaaa
    33661 tccgtactcg tcctggtcaa catcaccgtc aggcggtatt ggatcagttt gctaaaatcg
    33721 ttccaaccgt actgaaagaa gaaggttgcc acggctatgc gccaatggtg gattgcgctg
    33781 ctggcgtgag tttccagtct atggcaccgg attctatcgt gatgattgag cagtgggaaa
    33841 gcatcgcgca ccttgaagcg catctgcaaa ccccgcacat gaaggcgtac agcgaagccg
    33901 taaaaggcga cgtgctggag atgaatattc gtattctgca gccagggatt taatcctgcc
    33961 ttgtttgccc ggccatcctg accgggcaat gttctttcct ttaaacctca atctccgcca
    34021 tgtcgccttt ctcttgcaac cagttgcggc gatcttccga gcgtttcttc gccagcagca
    34081 tatccatcat cgcgtcagta cgctgatcgt cttcatcatc gatagtcaac tgcaccagac
    34141 ggcgagtgtt cggatcaagc gtggtttcac gcaattgcat cgggttcatt tcccccagac
    34201 ctttaaaacg ctggacgttc ggcttgcctt tcttgcgttt taattgctca agtacgccct
    34261 ctttctcttc ttccgtcagc gcgtaataaa cctctttccc gagatcaata cggtagagcg
    34321 gtggcagtgc gacgtaaacg tgaccgtgtt tcaccaacgc gcggaagtgt tttacgaaca
    34381 aagcgcagag cagcgtggca atgtgcagac catcagagtc cgcatccgcg aggatacaga
    34441 ttttgccata acgaagctgg ctcagatcgt cgctgttagg atcgataccg atcgctaccg
    34501 aaatatcatg cacttcctgc gaggccagca cttcgtcgga agaaacttcc caggtgttaa
    34561 ggatcttacc tttcagcggc atgatcgcct gatattcacg atcgcgcgcc tgcttggcag
    34621 atccgcctgc ggagtcacct tccacgagga acagttcggt acggttaagg tcctgcgcgg
    34681 tacaatcagc cagtttgcca ggcagcgccg ggccgctggt cagcttttta cgcaccactt
    34741 ttttagccgc acgcatacga cgctgggcgc tggaaatcgc catctctgcc agcaactccg
    34801 ccgcctgaac gttctggttc agccacagga taaaggcatc tttcaccacg ccagaaacga
    34861 atgccgcgca ttgacgcgaa gagagacgct ctttcgtctg cccggcaaac tgcggatcct
    34921 gcattttcac cgacagaaca taggcgcagc gatcccagat atcttccgcc gacagcttca
    34981 cgccgcgcgg cagaatgttg cgatattcac agaactcacg catcgcgtcc aacaggccct
    35041 gacgcaggcc attaacatgg gtaccgccct gcatcgttgg gatcaggttg acgtagcttt
    35101 cggtcagcag ttcaccgcct tccggcagcc acagtagcgc ccagtccaca gcttcagtat
    35161 cgccagcgaa attaccgata aacggttttt ctggcagcgt cggtaaaccg tttaccgctt
    35221 ccgccaggta atcattcaga ccgtcctgat agcaccagcg ctgttcggtg ttgttgatct
    35281 catctttaaa ggtaatctca acgccggggc acagtaccgc tttggctttc agcacatgcg
    35341 tcaggcgtga aacagaaaat cgcgggctgt caaagaaggt ttcatccggc cagaagtgca
    35401 cgctggtacc ggtattgcgt ttaccgcaag tgccgacaac ctgtaaatcc tgcacctttt
    35461 cgccattttc aaaggcgatg ttataaacct gaccatcgcg gcgcacgtta acttcaacgc
    35521 gcttcgacag ggcgttaacc accgaaatcc ccacgccatg caggccgcca gagaactggt
    35581 aatttttgtt agagaattta ccgcctgcat gcagacggca aagaatcaat tcaaccgccg
    35641 ggacaccctc ttccgggtga atatccaccg gcatcccgcg cccatcgtca ataacttcta
    35701 acgactggtc agcatgtaag attacatcca cgcgttttgc gtgacccgcc agtgcttcat
    35761 cgacactgtt atcaataact tcttgcccca aatggttagg gcgagtggta tcggtataca
    35821 tccccggacg gcggcgaacc ggctcaagcc cggtgagtac ctcaatggca tcagcgttat
    35881 aagtttgcgt catggtttaa gttagcaatt cgagttgatc gtcagagatg gtgcagacca
    35941 agaaaatcga cgatcgggtt gaaataatct tcgaagcccg tgaatgcgtg gttgccgcct
    36001 tctatgacag tctggcggca ggaagcgtag tacgccaccg cctggcggta atccagcact
    36061 tcatctcccg tctgttgcag cagccagatc aaatccggcg cttccagcgg gtcaatctgc
    36121 atgactttga gatcgtaaat atggcgtgac tctagcacat attgctgccc ggtgtatggg
    36181 ttctcgttct gaccgagata gtccgtcagc agttcaaacg ggcgcaccgc cgggtttacc
    36241 accactgcgg gcagcataaa acattgtgac aaccaggtgg cgtaatatcc ccccagtgac
    36301 gaaccgacaa tacccagcga atcgccgcca tgttccagga caatggattc cagcagttct
    36361 gccgcgtcgg aaggatacgg cggcaactgc ggaatgatca tctcaacgtc agggtgatgt
    36421 tccgccagcc agttttttaa caagctcgct tttgcagagc gcggcgagct gttgaaaccg
    36481 tgtaaataaa gaagcgtaga catcagtagc cttctgaagc ggtatcaggt tggaaacgtg
    36541 tgtccgccag gcgatgcacc tcggtggtca gcgtgccatc agcatgtaac tcgagagtac
    36601 gccagccggg cgcgatagta tccagcgtaa agttggaaca gtgcggctta aactgcacac
    36661 aggtcgacgg cgttgccagc aggcggcgac cgttccagtc gagatccagc tcctgatgaa
    36721 tatgaccgca cagcaagtat ttgacgtgcg gaaacttcgc cagcacggta tccagttcgc
    36781 ccgcgttacg cagactgtgt tgatcgagcc aactacaacc cgcaggtagc ggatgatgat
    36841 gcagcagcag caacgtatgg cgttctggcg catcggccag tttacgttcc agccactcaa
    36901 gctgaaactc gctcagctca ccgtgcggca cgccaaacac ctggctatcc agcaacagga
    36961 tttgccattg ctcaccaata aacacccgct tcgccgggga gatacccgca tcctgtaacg
    37021 cgctgtacat cgcgggctgg aaatcgtggt tgcccggcag ccagacgcag ggcgcacgaa
    37081 aacttgcgat gccttcagcg aaatgctgat aggccgcaga ggattgatcc tgcgctaaat
    37141 cacctgtcgc gacaatcagg tcgaattcgt gctgatgtgg acgaatcgcc tccagcaccg
    37201 cctggtagct ttcccaggtg tttaccccta acagagcttc gtgcttttgt gcaaacaggt
    37261 gagtgtcggt aatttgtaaa atcctgactc tggcctcacc agccagagga agggttaaca
    37321 ggctttccaa atggtgtcct taggtttcac gatgctagta aaccggaatc gccatcgctc
    37381 catgtgctaa acagtatcgc aaccagtccg ctaaaaactg attaatttga tgcttttcgt
    37441 cgcgttgatg caacttttta ttaggataat cataccgcgc tttgaagcga aaaatctgct
    37501 ggcttgaaca cacttcagcc accatcgcgt catgatacag acgcaccgtc attgacggaa
    37561 ggctccagta actgatcgcg ggcgcagtct gttctattgt caccagggta gtgtatcggg
    37621 tcgattccac aatcgtcagc cgatattgtg cgtttgccac ctgatagctt acagtttcgc
    37681 cgggtgcgtc attgcgcggt aacaaacggc gcaattgtga aaagttcatc tcgcacaggc
    37741 gcatcatttc aggaaagtca ggtgtgtaac gcttcattta tgcccactca ttttttaacg
    37801 cttgatgatg cagctgcagc cattgcaaag cgatgaccga cgctgcgttg tcgattttcc
    37861 cctcttctac ccactggtat gcctgttccc ggcttaccac atgaacgcga atatcttcgt
    37921 tttcatcagc cagaccatga ataccgcttg cggtcgtggc gtccacttcg cccaccataa
    37981 ttgacgaacg ctcactggtg ccccccgggc ttgccaggaa acttaacacc ggtttggtcc
    38041 gtttgactat cagtcccgcc tcttcaatcg cttcgcgacg ggcaacatct tccacacttt
    38101 caccctcttc tatcatcccg gcaaccatct ccagtagcca gggggtttca ctggtgtcgt
    38161 atgcggcaat ccgaatctgc tcaatcagca caacttcatc gcgcactggg tcaaagggta
    38221 gcaagactgc ggcgtgaccg cgctcaaaaa tttcccgccg tacctcatga ctcatttgcc
    38281 cgttgaatag acgatgacga aatctataaa gatctaatga aaaaaagccg cgataaagtg
    38341 tttctcgtgc aataatttct acatcgtttt tgccaaatgt aacgggcagg ttgtctggct
    38401 taagcattgt taatgtcctg gcactaatag tgaattaaat gtgaatttca gcgacgtttg
    38461 actgccgttt gagcagtcat gtgttaaatt gaggcacatt aacgccctat ggcacgtaac
    38521 gccaaccttt tgcggtagcg gcttctgcta gaatccgcaa taattttaca gtttgatcgc
    38581 gctaaatact gcttcaccac aaggaatgca aatgaagaaa ttgctcccca ttcttatcgg
    38641 cctgagcctt tctgggttca gttcgttgag ccaggccgag aacctgatgc aagtttatca
    38701 gcaagcacgc cttagtaacc cggaattgcg taagtctgcc gccgatcgtg atgctgcctt
    38761 tgaaaaaatt aatgaagcgc gcagtccatt actgccacag ctaggtttag gtgcagatta
    38821 cacctatagc aacggctacc gcgacgcgaa cggcatcaac tctaacgcga ccagtgcgtc
    38881 cctgcagtta actcaatcca tttttgatat gtcgaaatgg cgtgcgttaa cgctgcagga
    38941 aaaagcagca gggattcagg acgtcacata tcagaccgat cagcaaacct tgatcctcaa
    39001 caccgcgacc gcttatttca acgtgttgaa tgctattgac gttctttcct atacacaggc
    39061 acaaaaagaa gcgatctacc gtcaattaga tcaaaccacc caacgtttta acgtgggcct
    39121 ggtagcgatc accgacgtgc agaacgcccg cgcgcagtac gataccgtgc tggcgaacga
    39181 agtgaccgca cgtaataacc ttgataacgc ggtagagcag ctgcgccaga tcaccggtaa
    39241 ctactatccg gaactggcgg cgctgaatgt cgaaaacttt aaaaccgaca aaccacagcc
    39301 ggttaacgcg ctgctgaaag aagccgaaaa acgcaacctg tcgctgttac aggcacgctt
    39361 gagccaggac ctggcgcgcg agcaaattcg ccaggcgcag gatggtcact taccgacgct
    39421 ggatttaacg gcttctaccg ggatttctga cacctcttat agcggttcga aaacccgtgg
    39481 tgccgctggt acccagtatg acgacagcaa tatgggccag aacaaagtgg gcctgagctt
    39541 ctcgctgccg atttatcagg gcggaatggt taactcgcag gtgaaacagg cccagtacaa
    39601 ctttgttggt gccagcgagc aactggaaag cgcgcatcgt agcgtcgtgc aaaccgtacg
    39661 ttcctccttc aacaacatta atgcatctat cagtagcatt aacgcctaca aacaagccgt
    39721 agtttccgct caaagctcat tagacgcgat ggaagcgggc tactcggtcg gtacgcgtac
    39781 cattgttgat gtgttggatg caaccaccac gctgtacaac gctaagcaag agctggcaaa
    39841 tgcgcgttat aactacctga ttaaccagtt gaatattaag tcagctctgg gtacgttgaa
    39901 cgagcaggat ctgctggcac tgaacaatgc gctgagcaaa ccggtttcca ctaatccgga
    39961 aaacgttgca ccgcaaacgc cggaacagaa tgctattgct gatggttatg cgcctgatag
    40021 cccggcacca gtcgttcagc aaacatccgc acgcactacc accagtaacg gtcataaccc
    40081 tttccgtaac tgacgacgac gacggggctt cggccccgtc tgaacgtaag gcaacgtaaa
    40141 gatacgggtt atctgccgca ttcttccccc ttctcgcttc aatttcgacc agccatcctc
    40201 tattctgatg ggtatttacc actggtcccg gaagacaaaa atgaaacgga caaaatccat
    40261 acgccacgca tcgttccgca aaaactggag cgcacgccat ctgacaccag tcgctctcgc
    40321 ggttgccact gtttttatgc tggctggctg tgaaaagagt gatgaaacag tgtctctcta
    40381 tcaaaatgct gacgactgtt cagctgcaaa cccaggcaaa agcgccgaat gtaccaccgc
    40441 gtacaacaat gcgctgaaag aagccgaacg tactgcgccg aaatacgcca cccgtgaaga
    40501 ctgtgttgct gaatttggtg aaggtcagtg ccagcaggca ccagcccagg ctggcatggc
    40561 accagaaaac caggcgcagg cccagcaatc cagcgggagt ttctggatgc cgctgatggc
    40621 cggttacatg atggggcgtc tgatgggcgg cggcgcggga tttgcacagc agccgctgtt
    40681 ctcctcgaaa aacccagcca gcccggctta cggtaaatat accgacgcga cgggtaaaaa
    40741 ctatggcgca gcccagccag gccgcaccat gaccgtaccg aagacagcca tggcaccaaa
    40801 accggcgacc actactaccg ttacccgtgg cggttttggt gaatctgttg ccaaacaaag
    40861 cactatgcag cgtagcgcaa ccggcacctc ttctcgttca atgggtggct gataccgatg
    40921 gaaagagtca gtattaccga gcgcccggac tggcgcgaaa aagcccacga atacggtttc
    40981 aattttcaca ccatgtacgg cgagccgtac tggtgtgaag atgcttacta caagttgacc
    41041 ctcgcccagg ttgaaaagct ggaagaagtc accgccgaac tgcaccagat gtgcctgaaa
    41101 gtggtggaaa aagtgatcgc cagcgatgag ctgatgacca aattccgcat tccaaaacgc
    41161 acctggagtt ttgtgcgtca gtcatggctg actcaccagc catcgcttta ttcgcgtctt
    41221 gatctggcat gggatggcac tggcgaacct aaactgctgg aaaataacgc cgatacacca
    41281 acgtcactat acgaggcggc gttctttcag tggatctggc tggaagatca gcttaacgcc
    41341 ggtaacttgc cggagggcag cgaccagttt aacagtctgc aagaaaagct aatcgatcgc
    41401 tttgtcgagt tgcgtgaaca gtatggcttc cagttgctgc atctcacctg ctgtcgcgac
    41461 acggtggaag atcgcggaac cattcagtat ttgcaggact gcgcgacgga agctgaaatc
    41521 gctaccgagt tcctctacat tgacgatatc gggttaggtg aaaaaggtca gttcacggat
    41581 ttacaggatc aggtaatttc caacctgttc aagctgtatc catgggaatt tatgttgcgt
    41641 gagatgttct ccaccaagct ggaggatgca ggcgtacgct ggctggaacc ggcgtggaag
    41701 agcattatct ccaacaaagc gcttctaccg ctactatggg agatgttccc gaatcacccg
    41761 aacctgctgc ccgcttattt tgcggaagat gatcatccgc aaatggaaaa atatgtggtt
    41821 aaaccgatct tctcccgtga aggcgcaaac gtgtcgatca ttgagaacgg caaaaccatt
    41881 gaagcagcgg aaggtccgta tggcgaagaa gggatgattg ttcagcaatt ccacccgtta
    41941 ccgaaattcg gcgacagcta tatgctgatt ggtagctggc tggtgaacga tcaacctgcc
    42001 ggaattggca ttcgtgaaga ccgtgcgttg atcacccagg atatgtctcg gttttatcca
    42061 catattttta ttgagtaatc aatgatgccg gatggcattc gccatccggt aattgttaac
    42121 ctatctgcac cgacagcata ctcaggctgc ccatttctat accatcgacc ggaatggtaa
    42181 ttggctcctg cccatcccac gcacctaaga catacaacaa cggcagatag tgctccggcg
    42241 ttgggttcga taacgcgcca ccttcatggt caaggtaatt caccagagga tgctgctcta
    42301 ccgccccttg ccacgtcaga ttcgctttca catactcatt aaacgacatc gcccaaggat
    42361 atggtgaact atcaccgtgc cacttcactg tacgcaggtt atgcaccacg ttgccactgg
    42421 cgaccaacat tattccttca tctcgcagcg ctgccagttt gcgccccatt tcgaaatgcc
    42481 aggcggcagg tttgctactg tcgatactca actgcaccat cgggatatcg gcgtcaggat
    42541 acatcttaat cagcacgccc caggaaccgt ggtcaaagcc ccaggcttct ttatccagcg
    42601 tcaccgggac cgacgctaac agctcaacca gatgctgtgc cagcacaggc gaacccggag
    42661 caggataatg cgtatcgtac agcgcctgcg ggaagccacc aaagtcatga atcgtgggcg
    42721 gcgtctccat cgcggtcact cctgttccac gggtaaacca gtgagccgaa accaccacaa
    42781 tcgcttgcgg gcgtggcaat gtcatcccca acttctgcca gctgcgggta tacaaattat
    42841 cttccagcac gttcatcgga ctaccgtgac ctaaaaacaa tgctggcata cgtgttgaag
    42901 acatgatgat atccttaact aaaggtgtca ttttgatatc ctcacaatac gcttgttcgg
    42961 cggagtaaga acccggataa caatgatgat gatcatcagt tattttgacg atctgcctga
    43021 aggtgaagat ttataaggag ttgtcgatgt cagtacctct cattctgacc atactggcgg
    43081 gggcagccac gtttattggc gcgtttctcg gcgttctcgg gcaaaaaccc tcgaaccgct
    43141 tactggcgtt ttcgctagga tttgcggcgg ggatcatgtt gctcatctca ttaatggaaa
    43201 tgcttcctgc cgcactagca gctgaaggaa tgtcgcctgt gttgggttat ggaatgttta
    43261 tcttcggtct gcttggctat tttggcctgg accgcatgtt gccacatgcg catccgcagg
    43321 atttaatgca aaaatcggtg cagccgttgc caaaatcgat caagcgcaca gccattctgc
    43381 tcactctcgg catcagtctg cataacttcc cggaagggat tgccaccttt gtcacggcga
    43441 gcagcaacct ggagctggga tttggcatcg cactggccgt cgcgttgcac aatatccctg
    43501 aaggtctggc agtggcaggc ccggtttatg cggcaacggg gtctaaacgc accgcgattc
    43561 tgtgggcggg gatttctgga ctagcagaaa ttcttggtgg tgtgctggcg tggttaatcc
    43621 tcggtagcat gatttccccg gtggtcatgg cggcaatcat ggcggcggtt gcaggaatta
    43681 tggtggcgct ctcggttgat gaattaatgc cgctcgccaa agagattgac cctaataata
    43741 accccagcta tggcgtacta tgtggaatgt cagtgatggg attcagttta gtgctgctac
    43801 aaacggcggg aattggttaa aaacaacatc agaagttgtc cagcacatgg acaacttctg
    43861 ccagcttcac gaaagcctta ttctaccttt tcggctgtct cttctctcgt actgtttaag
    43921 tatttgttcc gcgcttcaag cagtgcgtct ctgtcctggc gaaacgcccg acaataacgt
    43981 ttcaaatgca ggaaataacc caccacaacc agagcaatca atacaatcca gtaccaggcg
    44041 ataaacatcc ttttaccctt aatatttaac aatactaaac atttagcgca taaatttcac
    44101 atatcctttt tcggatatat tcatatggtc gcataataac ggaacaatta tatgattttg
    44161 atcataatga aaaagaatgc ctgatattaa tgaaattttc tcatagaaag agaagcggaa
    44221 ttaacatctt gcatagcagg gcaaaataaa aaccgaagcc agctacaact tcggtttctt
    44281 gattaaggca gtaattaagc agcggttttc agctggcttt acgctcatgt gcctgacggt
    44341 atgccaccag gtcttcaata gtcacgagcg ccatattgtg tttattggca aactcaatgc
    44401 actctggtgc acgcgccatc gtgccatcgt cattagtcag ctcacacagc acaccagccg
    44461 gtttaaagcc tgccagcgtc atcaaatcaa tagtcgcttc agtatgaccg ccacgcgtca
    44521 gtacaccacc tgcctgcgcg cgaagtggga aaacgtggcc cgggcgattc agatcagaag
    44581 gttttgcgcc atcggcaatc gctgcgcgaa cggtcgtaat acgatcagcg gcagaaacac
    44641 cggtagtcac accttcagcc gcttcaatgg tcacggtaaa accggtgcca taggcgctgg
    44701 tgttattttc taccatcatt ggcagatcaa gttgtttacg gcgatcttca gtgatgcaca
    44761 ggcaaacaat accgctaccg tggcgaatgg tcagcgccat ctgctcaacg gtcatggttt
    44821 ctgccgggaa gatcatatcg ccttcgtttt cacggtcttc atcatcaagc accattacac
    44881 cgcgtccttc acgcagcgca gccagtgcat tttcaacacg ttcgaaaggc gtaccaaaag
    44941 aggaaagtag cgtctgattc atggtaaaaa aacctcacta aaattatggt taccagaatc
    45001 agggcagtct taggagtggc ggcatatagc caaaataacg tgagcgggtc catgcccgac
    45061 agaatcgtta ctctctccca tccggactct aaccgtcggc cccggaatta caccggatct
    45121 gctgtccttt gagttcgcac ccaaagcgct cgcgggcttt caactgagtt gatttaccgc
    45181 cggtggggaa tttcgccccg ccctgagaat aagcggattc actataacgc taatgattag
    45241 cggcagcaac gcatagcttc acataattct ggtttatgac ttacccttat cgcactacaa
    45301 tggtactcaa cacctatcag tacagggaaa ctacaatgat tgacccgaaa aaaattgagc
    45361 aaatcgctcg ccaggttcac gaatcaatgc ctaaaggaat cagggagttc ggggaagatg
    45421 tggagaaaaa aatccgccaa accctacaag ctcagctgac gcgcctcgac cttgtaagcc
    45481 gtgaagagtt cgacgtccaa acgcaagtcc tgttacggac tcgtgagaaa ctggcgcggc
    45541 ttgaacaaag aatgagcgaa ctggaaaatc gcagtactga gattaaaaag cagccagatc
    45601 cagagactct ccccccaacg ctgtaaacat ttgtctttaa tgcactgtaa attatgaatt
    45661 aatcttctaa tttacagtgc tttctattac cttatcgctt aattccattc agaatatcat
    45721 cacaaaaa
//
LOCUS       DAEEKQ010000012        44448 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.012, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000012 DAEEKQ010000000
VERSION     DAEEKQ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 44448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.012"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..716)
                     /locus_tag="KLK57_000708"
     CDS             complement(<1..716)
                     /locus_tag="KLK57_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE0891174.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSSA"
     gene            complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="KLK57_000709"
     CDS             complement(730..1389)
                     /gene="pgmB"
                     /locus_tag="KLK57_000709"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HBE0891175.1"
                     /translation="MKLQEVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVNAVLPGIRSLL
                     ADLRAQQISVGLASVSLNAPTILDALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGITGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="KLK57_000710"
     CDS             complement(1386..3653)
                     /gene="ycjT"
                     /locus_tag="KLK57_000710"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HBE0891176.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCKVSGDVQQCYTAKERRLLQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEVHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGGSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTAATHQEDE"
     gene            complement(3650..4705)
                     /locus_tag="KLK57_000711"
     CDS             complement(3650..4705)
                     /locus_tag="KLK57_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE0891177.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPLAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLREQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQRKMADDNRHLRSMEAFINHVQGKPVMIADAKQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(4715..5503)
                     /locus_tag="KLK57_000712"
     CDS             complement(4715..5503)
                     /locus_tag="KLK57_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HBE0891178.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGDKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(5521..6573)
                     /locus_tag="KLK57_000713"
     CDS             complement(5521..6573)
                     /locus_tag="KLK57_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HBE0891179.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKVRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPEGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSAFRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(6604..7207)
                     /locus_tag="KLK57_000714"
                     /pseudo
     CDS             complement(6604..7207)
                     /locus_tag="KLK57_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
     gene            complement(7194..8075)
                     /locus_tag="KLK57_000715"
     CDS             complement(7194..8075)
                     /locus_tag="KLK57_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HBE0891180.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(8096..9406)
                     /locus_tag="KLK57_000716"
     CDS             complement(8096..9406)
                     /locus_tag="KLK57_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0891181.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTHVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKTVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDMPGSNDIMEVKDAFMNGTAPMAIYSTYIL
                     PAVIKEGDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKAEETEAAEKFVTFMEQADNI
                     ADWVMMSPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNF
                     TRMGDVTGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(9441..10951)
                     /locus_tag="KLK57_000717"
                     /pseudo
     CDS             complement(9441..10951)
                     /locus_tag="KLK57_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
     gene            complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="KLK57_000718"
     CDS             complement(11163..11477)
                     /gene="pspE"
                     /locus_tag="KLK57_000718"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HBE0891182.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="KLK57_000719"
     CDS             complement(11552..11773)
                     /gene="pspD"
                     /locus_tag="KLK57_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HBE0891183.1"
                     /translation="MNTRWQQAGQKVKPGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="KLK57_000720"
     CDS             complement(11782..12141)
                     /gene="pspC"
                     /locus_tag="KLK57_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HBE0891184.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="KLK57_000721"
     CDS             complement(12141..12365)
                     /gene="pspB"
                     /locus_tag="KLK57_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HBE0891185.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRDELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="KLK57_000722"
     CDS             complement(12419..13087)
                     /gene="pspA"
                     /locus_tag="KLK57_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HBE0891186.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVNDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            13254..14231
                     /gene="pspF"
                     /locus_tag="KLK57_000723"
     CDS             13254..14231
                     /gene="pspF"
                     /locus_tag="KLK57_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HBE0891187.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNAVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQKGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="KLK57_000724"
                     /pseudo
     CDS             complement(14349..15614)
                     /gene="puuE"
                     /locus_tag="KLK57_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
     gene            complement(15652..16932)
                     /gene="puuB"
                     /locus_tag="KLK57_000725"
     CDS             complement(15652..16932)
                     /gene="puuB"
                     /locus_tag="KLK57_000725"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HBE0891188.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPYYPFPGGRTLRVPFTAMGAAWYSLRDRLGV"
     gene            complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="KLK57_000726"
                     /pseudo
     CDS             complement(16934..18424)
                     /gene="puuC"
                     /locus_tag="KLK57_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
     gene            complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="KLK57_000727"
     CDS             complement(18574..19131)
                     /gene="puuR"
                     /locus_tag="KLK57_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HBE0891189.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="KLK57_000728"
     CDS             complement(19158..19922)
                     /gene="puuD"
                     /locus_tag="KLK57_000728"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HBE0891190.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            20134..21495
                     /gene="puuA"
                     /locus_tag="KLK57_000729"
     CDS             20134..21495
                     /gene="puuA"
                     /locus_tag="KLK57_000729"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HBE0891191.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRDAEGNLQPPC
                     APGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIPADGAVAEASPGQFEINLYH
                     TDNVLEACDDALALKRLVRLMAGKHKMHATFMAKPYEEHAGSGMHIHISMQNNRGENV
                     LSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRRFQPGMYVPTQASWGHNNRT
                     VALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILHGLDNELPLQEEVEGNGLEQ
                     EGLPFPIRQSDALGEFIENDHLRRYLGKRFCHVYHACKNDELLQFERLITETEIEWML
                     KNA"
     gene            21644..21808
                     /locus_tag="KLK57_000730"
     CDS             21644..21808
                     /locus_tag="KLK57_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891192.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            21798..23183
                     /gene="puuP"
                     /locus_tag="KLK57_000731"
     CDS             21798..23183
                     /gene="puuP"
                     /locus_tag="KLK57_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HBE0891193.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTQMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            23317..23562
                     /locus_tag="KLK57_000732"
     CDS             23317..23562
                     /locus_tag="KLK57_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HBE0891194.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            23875..25518
                     /gene="sapA"
                     /locus_tag="KLK57_000733"
     CDS             23875..25518
                     /gene="sapA"
                     /locus_tag="KLK57_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HBE0891195.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            25515..26480
                     /gene="sapB"
                     /locus_tag="KLK57_000734"
     CDS             25515..26480
                     /gene="sapB"
                     /locus_tag="KLK57_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HBE0891196.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            26467..27357
                     /gene="sapC"
                     /locus_tag="KLK57_000735"
     CDS             26467..27357
                     /gene="sapC"
                     /locus_tag="KLK57_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HBE0891197.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            27357..28349
                     /gene="sapD"
                     /locus_tag="KLK57_000736"
     CDS             27357..28349
                     /gene="sapD"
                     /locus_tag="KLK57_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HBE0891198.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            28351..29157
                     /gene="sapF"
                     /locus_tag="KLK57_000737"
     CDS             28351..29157
                     /gene="sapF"
                     /locus_tag="KLK57_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HBE0891199.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            29225..29578
                     /locus_tag="KLK57_000738"
     CDS             29225..29578
                     /locus_tag="KLK57_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HBE0891200.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     PEIILQELNRRSPSP"
     gene            29946..30734
                     /gene="fabI"
                     /locus_tag="KLK57_000739"
     CDS             29946..30734
                     /gene="fabI"
                     /locus_tag="KLK57_000739"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HBE0891201.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            30879..32005
                     /locus_tag="KLK57_000740"
                     /pseudo
     CDS             30879..32005
                     /locus_tag="KLK57_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
     gene            32073..34007
                     /gene="rnb"
                     /locus_tag="KLK57_000741"
     CDS             32073..34007
                     /gene="rnb"
                     /locus_tag="KLK57_000741"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HBE0891202.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAKKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRGIIARPVA"
     gene            complement(34085..34188)
                     /locus_tag="KLK57_000742"
                     /pseudo
     CDS             complement(34085..34188)
                     /locus_tag="KLK57_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            34242..36232
                     /gene="pdeR"
                     /locus_tag="KLK57_000743"
                     /pseudo
     CDS             34242..36232
                     /gene="pdeR"
                     /locus_tag="KLK57_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            36379..36552
                     /locus_tag="KLK57_000744"
     CDS             36379..36552
                     /locus_tag="KLK57_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891203.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            36642..37391
                     /locus_tag="KLK57_000745"
     CDS             36642..37391
                     /locus_tag="KLK57_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE0891204.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            37660..37878
                     /gene="osmB"
                     /locus_tag="KLK57_000746"
     CDS             37660..37878
                     /gene="osmB"
                     /locus_tag="KLK57_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HBE0891205.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(38004..38330)
                     /gene="yciH"
                     /locus_tag="KLK57_000747"
     CDS             complement(38004..38330)
                     /gene="yciH"
                     /locus_tag="KLK57_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HBE0891206.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(38330..39067)
                     /gene="pyrF"
                     /locus_tag="KLK57_000748"
     CDS             complement(38330..39067)
                     /gene="pyrF"
                     /locus_tag="KLK57_000748"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HBE0891207.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(39260..40429)
                     /gene="lapB"
                     /locus_tag="KLK57_000749"
     CDS             complement(39260..40429)
                     /gene="lapB"
                     /locus_tag="KLK57_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HBE0891208.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(40436..40744)
                     /gene="lapA"
                     /locus_tag="KLK57_000750"
     CDS             complement(40436..40744)
                     /gene="lapA"
                     /locus_tag="KLK57_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HBE0891209.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGVAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(40893..41657)
                     /gene="pgpB"
                     /locus_tag="KLK57_000751"
     CDS             complement(40893..41657)
                     /gene="pgpB"
                     /locus_tag="KLK57_000751"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HBE0891210.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFCIKAAFVLFAILAAVIIVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            41827..42417
                     /gene="ribA"
                     /locus_tag="KLK57_000752"
     CDS             41827..42417
                     /gene="ribA"
                     /locus_tag="KLK57_000752"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HBE0891211.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(42481..>44448)
                     /gene="acnA"
                     /locus_tag="KLK57_000753"
     CDS             complement(42481..>44448)
                     /gene="acnA"
                     /locus_tag="KLK57_000753"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HBE0891212.1"
                     /translation="MLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLRKHGV
                     VEKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSEDQVE
                     LVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKVFAAS
                     NELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLLAKKA
                     VILGLKRQPWVKASLAPGSKVVSDYLAKAKLTPFLDELGFNLVGYGCTTCIGNSGPLP
                     DPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNINLAS
                     EPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEINVTRS
                     DTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGSIKPD
                     SPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTRHLPD
                     SDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESFERIH
                     RSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADGSQEV
                     VPCRCRIDTATELTYYQNDGILHYVIRNMLK"
BASE COUNT        10460 a        11768 c        11218 g        11002 t
ORIGIN      
        1 gccgatgatc caataaatcc ggcaacaaac atattcgctg gttggttata aaccgttttc
       61 ggcgcaccaa cttgctgaac aatcccatcc ttcataatca caatccgcgt cgccatggtc
      121 atcgcttcgg tctgatcgtg ggtcacgtag atcatggtgg tattcagctt ctggtgcagc
      181 ttgctgatct ctgcgcgcat ttgcacgcgc agcttggcat caaggttaga gagcggttca
      241 tccattaaaa acacgcccgc ttcgcgcaca atcgcccgcc cgagcgccac acgctggcgt
      301 tgcccgccgg aaagcgcgcc cggcttgcgt ttcaggtact cacgcaggcc gagaatttgc
      361 gccgcccagt tcacccgctc atcaatcacc tctttggcga ttttttgcat cttcaggcca
      421 aacgccatgt tgtcgtaaac cgtcatatgc ggatacaacg cgtagttctg gaacaccatc
      481 gctatattgc gtgctttggc tggaacgtca ttcattcgtt tgccgtcgat caacagatcg
      541 ccgccgctga tctcctcaag cccggcaatc atgcgcaggg tggtcgactt accgcagcct
      601 gacggaccga caaacacgat gaactcttta tcggcaattt ccaggttgaa gtccttcacc
      661 acatgcacct ggttatcgta gattttttga atatgttgta acgaaagctg agccatgttg
      721 attcctttgc tatacgtttt gccagaaggc cgataaccgc gaccaggtca gggagtccgt
      781 tgaaggcaat agtaattgcg ccccggttat gcccgcgccg atccccaccg agcgcatacc
      841 gctggcgtta atggcgtcaa tgcccgcctg cgcatcttca atgccgatac atgcctgcgg
      901 cggcacgccc agcccggcac aggcagcgag aaagatttcc ggatccggtt tcgagttttt
      961 aagttgggaa gcatccgcgc agaaggtgaa aaactcacgc agctccagtg catctaaaat
     1021 cgtcggcgca ttgagggaga cagaagccag ccctaccgag atctgttgtg cgcggagatc
     1081 tgccagcaaa gagcgaatgc cgggtagaac agcgttgacc gtcaactcgc gcaatgagtg
     1141 gacatagagc agatttttgc gatacgccag ttgcgccctc tcctgcgagt taaagtcgcc
     1201 ctctttgccc ccgtgttgca gaatgcgccg cagagactca tcgcggctga tcccttttag
     1261 ggattcgtta aactgcgcat caatgctgat gccaatttca gcggcaatct gctgccacgc
     1321 ctggaaatgc agatgcgcgg tatcggtgat tacaccatcc agatcgaaaa ttacctcttg
     1381 cagtttcatt catcctcctg atgtgtggca gcggtcccat tgaagggcaa aataaaatca
     1441 cccaaacaga aaacagattc ttcagccacg gatataagct gaccgttcaa acgcagtgaa
     1501 acgggggcag aagttcgaat cgcaatacgc tgcgcgtcaa gagtgacctg taattcgcag
     1561 ccctgccaga acagagggaa agacaactgt tgccactgct caggtaacgc cggattgaga
     1621 tgcaattcac cgtcacgcac gctccccccg gcaaaaccct gaatcgcccc cagccagata
     1681 gcgccagttg cggcagcatg gataccatca tcacaactat gcggatcagc accaagatcg
     1741 atttcagtcc cctcgcgcca gaactgataa ctttgggtca gcaggccaca gcgtgcggca
     1801 acaatgccgt ggattgcttt acttaatgac gagtcgtgaa tagtgcgcgg ttcataaaat
     1861 tgcagattgg caagacacga tgccgctgag aactgctccg gcagcatgta attgagcatc
     1921 accacatcag cttgtttgag gatttgcatc tcgttcactt ctgcgcgtga ataatccagc
     1981 agaatggttt gcttccccgc cgccgctttg tatttcgcaa gattaatcgc cggcttagcc
     2041 ataaacgaat catcctgcgg caaaacgccg tcgggctgaa tttctggcag ccgcagttct
     2101 ttaaggaaca tttcggcacg atgaataaac gcgtcgtcgc tacagccaaa ctggcgggca
     2161 atactcagcg cctgctggac gttgtaatac gccatatagc tggtgaaggc gttattattg
     2221 acatgttcgg tatattcgtc aggcccaata acatcatgaa tttccagacg gtcgttaacc
     2281 ctcaccgcgc ggctaatcca gaactttgca gtttccagaa gtagcgccat gccttcatgc
     2341 gcaatgaaac tttcatcccc cgtggtctgc cagtattgaa taaccgccca ggcgatatcg
     2401 gccaccagat gatgttccgc ctgcgccgag gccacttttt gccgcagccc ggtgcgaatg
     2461 ttaatggcgg caaattccgg cgtctcttct tcgccgctgc gcgcgctttc ccacggaaat
     2521 agcgcgccct gccagccgtt gcgtcgcgct ttctcctgcg cgcctggcaa gttgtgccag
     2581 cgataacgca gtaaacttcg ggcaaccgtc ggatcgctaa acagatgaaa cggtaacaaa
     2641 aatacttctg tatcccagaa aacgtggcct ttgtagcctt cgccggttaa gccttttgcc
     2701 gcaatgctgc tgcgctcgtc gtgggcaggc gtcatgatgc gcagatgata aagcgcataa
     2761 tctaacgctt gctgatcgtg cacttcgccg ccatttaccg tgatacgacg tttctgccac
     2821 cattgacgcc agttttctgt tgatactgca agaagttggt cgtaactctg ctgcgcgcac
     2881 atttcaagct ggcgaagcga cgcgctgccc cactcgtcta aaacggcttg cctgtcatcc
     2941 cgccagtcga tccacaccag tttttgcaac gtcactgtct cgcctgcatg aagctgcgca
     3001 ctggtatgtt gcagtaaacg gcgctctttg gcggtatagc attgctgcac atcaccgctc
     3061 accttacagc aacagctgat ggccacatca ctgcgtccat cctgggtggt gtagatcccc
     3121 tgcatcaaat gctgaccaaa cacccgcacc tgggtttcgt cgagatgctg gcgaccgtgg
     3181 ttggtctgcg tggcgtcaat gcctgttgaa atcagcactg acgcgtcggc gtccagtggc
     3241 gtaatagtga tttccagcac aatgagcggc agttggtctg ccgaaacaaa gcgacggctg
     3301 gcgatggtgt aacctgtgcc gttgctggta cgccagacaa cgctacggcg taattctcca
     3361 ctggcaaagt caagttcacg ctgccaggct tcgcgggata acgagaaaac ctcaccattt
     3421 atggcaatct ccatccccac gacatcaggc aggttcacca gttcgttgat ttcacctttt
     3481 cccgcccgat gatacagccc cgccagatac atccctcgcg tctggcgcgt gtaatcttct
     3541 tcatggctgg cgcgaagccc aagatagccg ttcccctgcg ccatcagcga tgcatacttg
     3601 ttcagggtat gctggctgaa atggggttct gataacgtta ctggcctggt cataattcca
     3661 cacgcgtccc tgtttctgcg gattgataca acgccgccac cagttgctgg atgatgtacc
     3721 cctgcttggc gtcggctatc atcacgggct tgccctgtac gtgattgata aaggcttcca
     3781 tgctgcgcaa atggcggttg tcgtctgcca ttttccgttg catcagcgtc attaattcac
     3841 cgttgttatc ggtgtagata tgtgctggaa acagcgtcgc accagcttta tcaccacaaa
     3901 agctgacgtt cataatcgac tgttcgcgga tgttgagtgc aaatgacgtt tccagccaca
     3961 gaatgccgcc gttatgaaat tcaatggtgc caaacagcga atcttcgacg ctgtaagttg
     4021 ccggatccca ctcaccaaat tgaccacagc tcttttgcgt gccgatcttt tgaaagctat
     4081 gcgcattcac gcttttcacc gccggaaagc ccagcacata catcgcagca tccagcatat
     4141 gaatgccgat gtcgatcagc gggccaccac cctgcagttc tttattggta aagacacccc
     4201 agccgggaac gccgcagcga cgcagggcgc gggcggtggt aacgtaaatc tctcccaaaa
     4261 cgccgttggt cacctgttca cgcagctgtt gcgtatcgag cgcaaaacga tggtgaaagt
     4321 cgtaggccag caccttgccc agtttgcgcg cggtatcgca catttcccgc gcctgttctg
     4381 gcgtcatggc gagcggtttt tcgcacatca catggcagcc cgcttccagt gccatcaggg
     4441 tatgttcgta atgaaaacgg ttaggtgagc agacgctaac cacatcaggt ttcaccgcca
     4501 gcagcatggc ctgcggatcg tcccacacgg atgcattccc gtatttttct gccagcgcct
     4561 gcgcctggga aaggcggctg tcacagacag ccaccagttc cagatcgttg cgggtgcagt
     4621 agtacgaagc atgaacctta tccgccacct ggcctgcgcc tattatcgcg acccgcagcg
     4681 gagagcttgt cattgcactt ttcacaaaga cctcttagca ggtacgcaac caggccaacg
     4741 aatcacggta cgcctgggca ggatcttccg cccggatacg cccttcgtac actacatagc
     4801 cctgatagtt atccgcgcgc agctgttcaa acagcgcgtg gaaatccagg gtgccgctgc
     4861 ccggctggta gcgatggtta tccgcaatat gcacatgacc gagcaggtcg cggttgtcat
     4921 gcagcgcctg cgccaggtta tcttcttcga tattcatgtg atagaaatcg ccgataatct
     4981 gtacatgttt aagatcgttt tcgacgatgt aacggcgggc atcggcgagg gtgttgatca
     5041 tatgatcctg atagcggttt aacggttcga gatacaccac tgttccggta cgcgcggcga
     5101 cctgttccag tacgcgcagg gaatcactca ccattttgcg gtcgccgtcc aggctacgcg
     5161 gcgaggtcat cggcggtaag cggaaggtaa acatgcccca cgctgccgga acgacgatac
     5221 ctttatcccc cacttctgcc agcgcttcga gaatgcgttc gatctgcttt aagccattaa
     5281 gacgacgctc ttcgataaag tcgccaatcc aaccgtcata gccaccgcag gcggtggtca
     5341 ccggtaaacc ggtttctttg attgctgctt tgacttcttc gagattgttg accagcagtt
     5401 tgccgtcaat ctcaaaacca tcgaagccca tctctttgat ataacgaaat ttctccagaa
     5461 tgttttccgg aaagaatgcc tgattctgtg tgcctatttt cattttttca ttcctttgaa
     5521 ttaaaacgta acgcccattt tgatgctctg ttccggatgc tgatcgacat actgcatgta
     5581 gctttccggg ctattggcaa aggtcactac cgggtcgatt aaatcttcgc aattgagata
     5641 accgttcatc agcagttccc agcaggtttc ttcaatacgc ttacggctcc agcgcggata
     5701 atccgggttc ggttcgctgc acgcgcggga gaaaacaatt ttggcgttat tgaaatgcgc
     5761 ttcgcgtccg aggttaaaac cttcagcaaa tggtttggca aacgcaacat aggagatggt
     5821 gccaccgtag gccagaccgc ggaacgccga ttgcagcgcg tcggcatagc cgctggtttc
     5881 gataatcaca tcggcaccct gcttgccggt cagcgttttg atctctttgc ctacgtcagt
     5941 accaatgggg ttaaggcaga aatccgcgcc gtggcgacgg gcaatatcac agcgatgagc
     6001 gataggatcg acgccaatca cgaccgacgc gccagcacgt ttagccagtt gaatggcaat
     6061 ttgaccaatc gcgccaagcc ctaccaccac aacaaaatca cccacgcgca cgttggcatc
     6121 gcgcacgccg ctcatggcaa actgcgccgg gtcgtaacaa acggcgtttt tccacgagct
     6181 gccttccggc attttgcgca gcttgtagtt attcaccgcg ttaatgatga ccgtctcgga
     6241 gagcgggccg tagccgcata cgctgtcgcc caccgcgtag tcggtaacgt cgctgccgca
     6301 ctcgataatg tcgccaacca ccatgtttcc aagctggaat ttgccaaact caatgccgcg
     6361 cggcgcatct gccggacgcg gagtgaacat ctgccattcg ccgttaaagt cttcatcgat
     6421 aaacgggctg gcggcgcgga agtcgaccac ttccgttccg tgtttcggtg cgccgaaacg
     6481 cacgcggacc ttcacttcat tagctaaaat cgcccgatct tcatactcaa ccagcgcagc
     6541 aacacgcggt gctgtggcta ctaacttttt catttaaaac tccttgtaaa gaaactggcc
     6601 gctttagccc ttcacgccac cggcggtcaa accactctta ataaaacgtt ccgaaagcgc
     6661 atacataatc accaccggca atgcggtcac cagtgaggcc gccatcatcc gtccccagat
     6721 gtaatctggc gtactgaaca gcgcgttcag gcccaccggt aaggtgaaat tgccggcgct
     6781 ggagaggaaa atcgacgcaa acagatagtc gttccacgcc accataaagc agtagacgaa
     6841 cacggatatc agcccggaca tcgccaacgg cacggtgatg cggaagataa tttgcagacg
     6901 gttgaggcca tccatcatcg ccgcttcttc gatctcatcg gggatggtgt cgaagtagct
     6961 tttcagcata aacacggcgg tgggcagagt ttgcgtcacc atggtgatga tcagcgccat
     7021 ctcagtcgac gtaatgctcc agcgtccact gctgcggcag cagcgtagga tgcagtgaga
     7081 tcgcctcttt cgcgcccttg aacgaggtca tcagcatcac aaaaaatgga aacagcgtga
     7141 tgatgagaaa cagcgccagc ccgcagtaaa aaccgatgcg actgagtgtg cgtttatttg
     7201 ttgccattga ggttcacccg tttcctggtc agcagaatga cagcgaaaat aatgatgaag
     7261 agcaccaccg aaatcgccgc cgctttccct aagtcattaa aggcaaaggc ggttttgtag
     7321 agatagacgc cgagaatatc gaccttggtg gtcagcagat aaacatcagc aaacatgtag
     7381 aacatccaga tagtgcgcag ggtcaccacc gtcgccagga ccggcataat tgcgggcagc
     7441 gtgacgatgc gaaaccgctg ccaggcatta gcgccatcca tctctgccgc ttcgtacagc
     7501 gatttgtcga tggtctgcaa aatcgccaga aacgagataa aggcatacgg gaagtagcgc
     7561 cagatggcga acagcaccac cagcacaaag ctactgcccg gattatcgaa ccacagcggt
     7621 gcctgctcat agagatgcag aagatcgacg ccgaggtagt tcacaatgcc gtagccgttg
     7681 ttgaacatgt atttccaggc aaacaccagc gaaatggacg gcgttacgta ggagaggatc
     7741 accagcgaac gcgcggtttt gcgcaggcgg aactcgcggt tgaaaaacat cgccactgcc
     7801 agcccgagca cggtgctgcc cgccaccacc agcgcggtat accagaccgt catccacagc
     7861 gagtgccaga agccgggatc ggagaggata cgcacatagt tactcacccc aacaaaggtt
     7921 gactcgatgt tgggattgag cggcagacgt aaaaaactga tttcgatatt cgacaccatt
     7981 ggccaggcca ccagaccacc cagcagtaat aagctgggcg cgagaagcag cagcgcaaag
     8041 ggcatatcgg aacgacctga aaaaagcctg ttcattgcgg attcctgtcc tgggtttagt
     8101 gctgttcgat cagttcatcc agctttttct ggctcgcttg cagcgtgcca gggagatcgg
     8161 ctttacccac ggtgacgtta tgcaccatgg agctcaccac gccagaaccc gtcacatcac
     8221 ccatgcgggt aaagttttta tcccctactg cgccaaagac ctgaatattt ggcagttcac
     8281 cgattagctg attcggtagt tcacccagcg ccttaataac gtcgttgtct ttccaggtgg
     8341 cggtagtgac aaccgcttta ttcaccggca gcgcagcacc tggcgacatc atcacccagt
     8401 cggcaatgtt gtctgcctgc tccataaagg tgacaaattt ttctgctgct tccgtctctt
     8461 cggccttttg cccggcggta atggtcagcg aggtcaacat gccgtagacc gcagagtttt
     8521 tctcggttgg caccacgaaa ccgacgtttt tcgggtcgcc ttctttaatt acagccggaa
     8581 ggatataggt ggagtaaatc gccatcggcg cggtgccgtt cataaaggcg tctttcactt
     8641 ccatgatgtc gttagaaccc ggcatgtcgt tagaacccgg catagtgttg gcagcaaggt
     8701 cgcggtaata ggtcagtgcc tgcatcatct ctggtgtatc aagggtgatt ttgccttcgg
     8761 cgttaaagac attagcctgg ttggataacg caaactggga gaaggattgt tccgtcaaca
     8821 cgctttctgc tgtaggcagc gcaatgccgt attttttatt cgccgggtca ttcagtttct
     8881 gtgcaacgtc cagtagctgt tgccagtttt tcggctcctc cagccccgct tttgccagca
     8941 catctttgcg ataccagatg ccgccaatcc aggcgctgac gggaacaccg gtccaggcac
     9001 tcccatcttc ggtgcgcaca atacgcagta cgccatcgta aaacgcgcct tcaccaacgt
     9061 tgctgatgac cgtggcaacc gtcttgcgat cgataagctg ctctttatcc ataactttgg
     9121 cgtagtcatg gctggtttcg atcacttccg gcagcgagcc gctacgtgaa agggtgatga
     9181 ctttggtgtt ataagcgtcc tcttccacgg gcacttgttt aacggtgata cctgggtttt
     9241 ctttttcaaa acgggcaatc aatttgctga taactgcctg acgctcctgc tccaccgaag
     9301 aatgcataaa ttcgatggat acatgggttt tattttcttc tttacagcct gaaatcaggg
     9361 cgcaggaaac cagtgctgat aacagcacga tttttgattt aatcatgaga tgttcctttt
     9421 tatttaatcc acataacctg ttaggggcgc agcgttattt cattacccaa tagatcaact
     9481 tcactaatca gatcccgacc atgcagatta gtaatattta catgctgaat atttttactg
     9541 acattaaaca atccagtcag acaatcacca tcagcgttac ttctttgaat acgcattacg
     9601 gatgaattaa tcgtatcgat ggtaaaatta ttatccggat gaaactcgtt atggctgcga
     9661 cgaagtgtaa ttaaacgcga caactcatga tataccgcat gccttaatgt agcttcatcg
     9721 ttcagttctc gggttatctc tttactgtga tattttttac ggttaatcgc acggttatat
     9781 ccgagttttt cgacacctgc gtaatcatta cgcgagccaa gaatactttg aatatatatc
     9841 gctggcacac cggggaaact taacaaaatc gcatgggcga gaataaacct ggcgcaacgt
     9901 tcttcatcgc tactctcacg gcggcttaac gcatccatat gtgagaggcg aggaagttaa
     9961 accaggtggt gttgctggaa ggtagtgtca ggttttgcgc ccacgcacaa agcgcctcaa
    10021 cgttctgttt ttgcaccgca tgcagcacca gtggcggcag cgagaactgg tacaccatat
    10081 gtgcttcgtc atcgcctgcg ccaaagtaag caatgttgtc tttatgcgga acattggtct
    10141 cggtaatgat cactgtacct ggcgcaacgt tatcaataat cgaccgtaac agtttgataa
    10201 tcagatgtgt tttttccaga tggatgcagc ttgttcccgg ctctttccac ataaagccaa
    10261 cggcatccag gcggacatac tcagcacctt tcgcaaggta acagagtaaa acatccacca
    10321 tcgccagcaa cacttcaggg ctacggtaat tcaggtcaat ttgatcgtca ctaaaggtgg
    10381 tccataaatg gcgcgttgaa tgatcgcgca tctggaatgg cgttaataac ggtaacgcac
    10441 gcgggcgagt gacggcgctg agatcggttt gcggatcaac cgcaataaaa aaatcttcaa
    10501 aacctggctg ctgttgtaaa tagtttttaa accattcact ttttgccgac atatggttgc
    10561 agacaaaatc aaacattaaa tgactgcatt caccgagttg ctgaatatcc tgccactccc
    10621 ccgcttcact ggcgacctga tgataatcaa ttaccgaaaa cccatcatca gatgaacatg
    10681 gataaaaagg taataaatga acatgtgaaa aaatgctttg cagccattga tggtaaaact
    10741 gattaaatgt gggtaatggt tttaaatcat tgctgtgaaa ttgatcggca taggtaatta
    10801 acacgacatc actttcatcc cagtgttttt tacgtcgctg tgtaattaat cgtttcgcac
    10861 tctcaagacg cgttacaagt ttctgtaaat gagttgcggt aaatgttcca ccgtagattt
    10921 cgtccaggta atccgtaatt ttctgtttca tgttgccttt ttgtggtagc ggtcccatga
    10981 aacgagagac tactccgcag aaaataaact gtcaattgag cacaagggtt ttagtcacgc
    11041 aggaaatcat tttttaccag ggaaaaagcg tatttgtgag ctgacgcaaa taatctatgg
    11101 ggatgtgaat aaggtgtgac agaaaaaaaa cggcgcataa gcgccgctca tggtgaattc
    11161 ttttaacctt tgaccttcgg cattgcgatg tctttcaggc caccggcgtt ctcaacgtgg
    11221 gtatatccca tctcgctaag gatctctttt gcttgccctg actggcgtcc ggcattgcaa
    11281 tacactttca cggtgtcgtt tttatccgga acggcggtgg caatgcgctc tttcacttcc
    11341 ttcaggggaa tattgatggc cccctgaacg tgctcttgct gatactgctc tggaacacga
    11401 acatcgatcc agtgttcagc ggcgaaaacg ggcagtgaaa acaccagtgc cagagcaagt
    11461 aagccttttt taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc
    11521 ctgagattta acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat
    11581 tagcagcccg acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc
    11641 gagcaactga ttttatcgcc cagcccgcta cacccgccgg gccatagcgc agtgcggtaa
    11701 gaagtaccag cttgcctgct aatttgaaac caggctttac cttttgcccg gcctgttgcc
    11761 agcgagtatt cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc
    11821 ggaagtgaca taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc
    11881 gacttcatcc agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg
    11941 cattggatca agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc
    12001 gaagaaaatc gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt
    12061 ggcaatcccg gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataatttttt
    12121 attgagatta atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata
    12181 gattccagcg cctgaatacg ttcgcgcatc cgttttgctt catcagccag ttgcgctaat
    12241 cgctgctgct cactttgcga caattcatcg cgaccagaac gattgctgta atgcagccat
    12301 aaccagatcg gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg
    12361 ctcataagta ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt
    12421 attgattgtc ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat
    12481 cggctttcag ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt
    12541 ctgcttccgc ttccatctgg tcaattcgac gttcgaaaga ttcgaaacga gccattgctt
    12601 catccagttt gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat
    12661 gacgtaacat caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc
    12721 caatctcttt cttcatgcgt gccagcgtat cgttcaccag cgtcacttca tgttccaggg
    12781 acttaatcag atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc
    12841 tctctttcag cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt
    12901 gttcaatacg gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta
    12961 cttcaaccag tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt
    13021 ccgctttctc taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa
    13081 tacccataat gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata
    13141 caatttgcgt gccaattttt tatctttttg atttataaag atctgattga agaatcaaca
    13201 gcaacatgcc aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg
    13261 aatacaaaga taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt
    13321 cgcatctcgc accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag
    13381 agctgattgc cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc
    13441 ttaactgcgc ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg
    13501 gggcgtttac cggtgcgcaa aaacgtcatc cagggagatt tgaacgtgcc gacggcggta
    13561 cgctatttct tgatgaactc gctacggcac cgatgatggt gcaggagaaa ttattgcgcg
    13621 tgattgagta cggtgaactg gagcgcgttg gcggcagcca gccattgcag gtgaatgtgc
    13681 ggttggtatg cgcgacgaat gccgatctcc cggcgatggt caatgaaggc acttttcgcg
    13741 ctgacctgct cgaccgactg gcttttgatg ttgtacaact gccaccactg cgcgagcgcg
    13801 aaagcgacat aatgttgatg gcagaacact ttgccatcca gatgtgtcgg gaaatcaagc
    13861 tgcctctgtt cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc
    13921 cgggaaatat tcgtgaattg aaaaacgcgg tggaacgttc agtgtatcgc cacggcacca
    13981 gcgattatcc gcttgatgac atcattattg atccctttaa acggcgtccg cctgaagacg
    14041 ctatcgccgt ttcagaaacc acctcgcttc cgacgctgcc gctggattta cgtgagtttc
    14101 aaatgcagca ggaaaaagag ttgctgcaac tcagtttgca aaaggggaaa tataaccaga
    14161 agcgcgcagc tgaattgctg gggttaacct accatcagtt ccgcgcgttg ttgaaaaaac
    14221 accagattta gcgaagttgg gcaatgcgat tcgccggatg cggcgtgaac gctttatccg
    14281 gcatacataa tcaacgtcgt ttgcaggcct gataagcgca gcgcatcaga cattattgcg
    14341 ttgggctatt aatcgctcag cgcatcctgc aaaattttca ttgccgcatc gaattgcgca
    14401 tccgggatgg tcagcggata caggaagcga atcacgttgc cgtatgcgcc acaggtcagc
    14461 aggagcagcc cctgcgccag cgcgcgttgc tggattttct gtgcaatcgc cgctgacggc
    14521 tcgcccgttt gcggatcgtt aaactctgcc gcaatcatcg accccaggcc gcgtaccgca
    14581 gcaatggccg gaacgctttc tttggcatca atcaacgtgt ttttgagacg ctggcccagt
    14641 tgattcgcgc gttcgcagag tgattctttg ttgataatat tgagcaccgc gtgcgcggca
    14701 gccaccgcca gcgggttccc ggcgtaggtg ccacccaacc cgcccggcgc gggcgcgtcc
    14761 ataatattcg cgttaccgac cacgcccgaa agcggcatcc cgcccgcgag gcttttcgcc
    14821 atcgtcatta agtccggctt atcggcgtaa tgatccatgg caaacagttt accggtacgc
    14881 gcaaagccgc tttgcacttc atcggcaatc atcacaatac cgtgctcgtc gcacaggcgg
    14941 cgaatggcgg caaccagctc ttttggcgca acgttgaaac cgccctcgcc ctgcaccggt
    15001 tcgaaaataa tggccgcgac ctgcttcgct tcgatgtctg atttaaacaa gcgttcgatg
    15061 gcgtcgaggg agtcctatgt tgaaatgccg tgtaaatctg acggataagg tacgtgatac
    15121 accgaaccag ggaacgggcc gaagccgatt ttgtacggcg caacttttcc ggtcagcgcc
    15181 atggtcatat atgtacgacc gtggaagcca ccgctaaacg caatcacgcc agggcctccg
    15241 gtatgggcgc gagcaatttt caccgcgttt tccaccgctt ccgcaccggt agtgaagaac
    15301 gcggttttgg cctgcccgct caccggggca agggcgttga ttttctccgc cagggtgacg
    15361 tagctttcat acggcacaat ctgatacgcg gtgtgggtaa actgttgcag ttgctgctcc
    15421 accgccgcga ccagatcagg gtggcgatgt ccggtattca gcaccgcaat gcctgcggcg
    15481 aaatcgatgt actcgttgcc ctcaacatcc ttcagagtag cgttttcagc cgactgggcg
    15541 aagaagttac acatcacgcc aaccccgcgt ggggtagcag aaagacgacg ctgatggaat
    15601 tcattgttgc tcatgtttca caccttttga ctcttcactg ttaatcggaa attaaacgcc
    15661 cagacgatcg cgcaggctgt accaggctgc gcccatcgcg gtaaacggta ctcgcagcgt
    15721 gcgcccgccg gggaacgggt aatacggcag attggcgaag gcatcgaaac gttcggcgtc
    15781 gccgcgcagc agttcggcaa tcaaacgtcc ggcgagatga gtacaggtga cgccgtggcc
    15841 gctgtaaccc tgcatgtaat agatgttggt atcaaggcga ccaaactgcg gcatgcgcga
    15901 cagggtcagc aggaagttgc ccgtccagcg gtagtcgatt ttgacgccct tcagttgcgg
    15961 gaaggttttc agcagtttcg gcacaacaag acgctcaacg tcatccgggt cgcgcgcgcc
    16021 gtagaccacg ccgccgccgt acagcaaacg gttgtcggcg gtaagacggt agtaatcaag
    16081 cagataatta cagtcttcca cacagtagtt tttcgggatc agcgaacggg ctaaatcttc
    16141 cgacagccgt tcagtggtga tcacctgcgt gccgcacggc atgctgcgtt tcgccagttc
    16201 cggctctact ttatcgccca gatacgcatt cccggcgaca atcacatact tcgccgtcac
    16261 ctgacctttg gcagtgcgca ccaccgctgg cgtggtgtgc tggatctgcg tcacggcaga
    16321 aagctcatac acgcgcccgc cgttcaggcg gatggcatcg gcttcaccaa tggcgaggtt
    16381 tagcgggtga atatgcccac cgctgtgatc caacagtgcg ccagtgtagc gatcgctggc
    16441 gacctcgcgg cgaatggcgt tagcgtccag cagctccagc tgtttattgc cgtagcgttc
    16501 ccagttctct ttctgctctt cgagtgtggc gagctgttta tcattcatcg ccacaaacag
    16561 gccgccgggg cggtagtcgc agtcaatctg atagcgtttg atacgttcgc ggatgatctc
    16621 gccaccctcg aacatcatgc tgccgagcat acgggcggtg tccatgccgt agcttttttc
    16681 gatcacgtcg atgtcgcggc tgtaggagtt cacaagctgc ccgccattgc gcccgcttgc
    16741 gccaaagccg atgcgtgagg cttcgagaac cactacgtca aagcccgctt ccgccagatg
    16801 cagcgcggag gagagcccgg tatagccgcc gccaaccacg caaacgtcgc aggtgattga
    16861 ctcattcagt gtgtcgaatg gtgcatattt attcgcactg gcggcgtagt aactgctggt
    16921 atgttcggtc atttcaggcc tccaggctta tccagatggt tttcagttca gtgaattttt
    16981 caagggcatg cagggatttg tcgcgaccgt tgccgctctg cttatagccg ccaaacggca
    17041 cggtcatatc gccgtcgttg tagttattga cgaagacgga accggctttc aggcggcggc
    17101 tcatacggtg cgcgcgggag aggtcgcgcg tccataccgc cgcgccaagg ccgtactggc
    17161 tgtcgttggc aagctgtagc gcctggtctt ctgatgtgaa acgcgtgacc accagcaccg
    17221 gaccgaaaat ctcttcgcgg cttaaggacg cattcgggtc aacatccaca aagatggtcg
    17281 ggccgatggc ggcagccagc tcagcgttac gaccatccag cagcagttgc cctttgcttt
    17341 cgccttcacg aataaagcta tggaccgagt cggcgtgggc gcaatcgatt aaggtgccca
    17401 tggtggttac ggtatcaagt ggatggcccg gctgccagtt ttgcgcctgc tgttttaaca
    17461 gggctaagaa ttcatcggcg atgctctctt ccagtaacaa acgcgttccg gcgatgcaca
    17521 cctggccctg gttgtagaag atgcctgcgg cggtggcgct tgccgctttt tgcagatccg
    17581 gacaatcggc gaaaacgatg ttggcgcttt tgccgcccgc ttccagccag acgcgtttca
    17641 tgttgctatc acccgcgtct ttcagcagtt gtttacccgt acgcgtggag ccggtaaagg
    17701 cgatggcatc gatatcgtta tgacgcgaaa gcgcctgccc ggcttcatga ccataaccgc
    17761 tcactacgtt cagcacgcca tccggcagcc ccgcttcttc ttttgccagc ccggcgagac
    17821 gaatcgcact gagtggtgat ttttcagacg gttttagaat cacgctgttt cccgccgcca
    17881 gcgccgggcc gagtttccag caagtcagca gcagcgggaa gttccacggc acgatggcgg
    17941 caatcacgcc gaccggttca cgcacgatca tcgccagctc atggctactg gtggtcgcca
    18001 cttcgccata cactttgtcg atcgcttcgg cgtaccagcg aatggcgcgc gccgcgccgg
    18061 gaatatcatc acgcagactg tgacgaatcg gtttgccggt gtcgagagtt tccagcagtg
    18121 ccagctcttc ggcgtgggct tccattaaat cggcgagttt attcagtacc gctttacgtt
    18181 tagccggaga agagagtgac cagtcgccgc gttcaaatac gccgcgtgct gcgctcaccg
    18241 catggtcgat atcgacgctc ttgccgcggg caattttcgc cagcggtgcc taggtgaccg
    18301 gatcaacggt ttcaaaggtt tcattttccg ccgcagcagt atattcaccg ttaataaata
    18361 agcggttttc aatggcgaga cttaacgctt tatcctgcca gtaagccaga tgatgaaaat
    18421 tcattatgac tcctgtttca cgtctatcag atatatgcag tttgtgaacg tcagggcgca
    18481 gagatccccg ggccgcatgc ccgtttattt ctggcgacat tctttatgga ttgccgggcg
    18541 cattgcgctt acccggctta cagaacaaaa agattaaaac gtggtgggcg tatgggcgct
    18601 gataattcgg caaatacctg ccgacgtatt actgaaactg tgcgggatgc cggtattaat
    18661 ggcataactt tgccccgcga cgaggtggta atcctgacca ttaatcgtca gaacaatttc
    18721 accttccagt actgtgccta tttcctcacc ctgatgctta attctttccc cagtggttgt
    18781 gcccggctgg tacgtttcaa agatcatcgc cagcgtgcga ttcgggttac cgttatgaac
    18841 cagcttcatt gacacaccct gactgcccat ctcaattaag tcgtcctgat taatgacgac
    18901 ctgcggctca tcaggttttt ccggctcgga aaagaattcc gagagtgaca gaccatacac
    18961 cttcagcagc ttttgcagcg tactgatggc agggctgact ttatcttgtt ctatcgtact
    19021 gatagcactg tgagtcagcc cggagagttc ggcggcacga cgttgtgaaa gcccctgctg
    19081 ctggcggatt tccgacaagc gttttcctgg cgccagtccc tcatcactca tatttgcatt
    19141 tccttaaact gtagtggtca gagtcgctgt ttttcagcga tatggtgctg acaagcggtg
    19201 ataaagccct cgaacaatat acgcgaaagc gcgtactcgc tactgttcca ttccgggtgc
    19261 cactgtacgc ccagcgcaaa aggatgattg atgacgctaa ccgcctcaac taagccatcc
    19321 ggcgagcgag cttcaacacg caaccgtggg ctaacgacct tcgccccttg tccatgtaga
    19381 gagtttaccc aaaagttgct acattcaggt aacaacgcag acagtaatcc cccctcttca
    19441 acctgaactt catgcgacgg tgcatattgc tgttccaccg gcagttcggg atcttcccga
    19501 tgttccagca attcaggctg ttcgcacagc ttgcgatgca acgaaccacc ggttgccacc
    19561 accagttctt gtaaaccccg gcagatggcg aaaatgggga tgcgcctttc gagtgcggca
    19621 ttaattatcg ccatgctcag aagatcacgc ccgggatcgg cgtcaggctc atcgccgttt
    19681 tcaccatata ggtgcggctg cacattgctg ggactaccag gaagataaat gccatcgagt
    19741 ttcggcaaaa gttgttcaag taacgacggt tccgccagcg catgtggtag cgcaattggc
    19801 aagccgcctg cgtggatgat ggcattcagg tacttttctt gcagagtctg ggtcgcatga
    19861 cccttaagcc tgttcctgca cattacgaca ccgataaccg gattgttcat tatattttcc
    19921 atgctcaatc tcacaaagtg gactaaatta tcgccattac tgcgctttaa atgaccttta
    19981 tgttcaatat tttttcaatc tagcagtggg ttttcatttt tgcaaactca atttaacatt
    20041 tgacaaacat ttagtttgca tacagattcg aatggtggtc attatatttt acgctttgat
    20101 aacgagcgga aaacaaacca aaggcgaaga atcatggaaa ccaatatcgt tgaagtagag
    20161 aactttgttc agcagtcaga agagaggcgg ggtagcgcct ttacgcagga agtgaaacgc
    20221 tacctggagc gctacccgaa tacgcaatat gttgatgttt tacttactga tttgaatggt
    20281 tgcttccgtg gaaaacgtat ccctgtttca agcctgaaga agctcgagaa agggtgttat
    20341 ttcccggcct cggtatttgc aatggatatt ctgggcaacg tagtagaaga agcaggtctg
    20401 ggtcaggaaa tgggcgagcc ggatcgtacc tgtgttcctg ttctcggttc cttaactcct
    20461 tctgccgccg atccagagtt tatcggtcag atgctcctga ccatggtcga tgaagatggc
    20521 gctccctttg acgttgagcc gcggaacgtt cttaaccgcc tgtggcagca gctgcgccag
    20581 cgcgacgctg aagggaatct gcaaccgccc tgcgcgcctg gcaccgatga ccgcaataca
    20641 caaagccagg tttactccgt tgataacctc aaccacttcg ccgacgtgct caatgatatt
    20701 gatgaactgg cgcagttgca gctgattccg gcagatggcg cggtcgctga ggcctcgccg
    20761 ggtcagtttg aaatcaacct ttaccatact gataacgtgc tggaagcctg cgatgatgcg
    20821 ctggcactaa aacgcctggt gcgtctgatg gcaggaaagc ataagatgca cgccactttt
    20881 atggcgaagc cgtatgaaga gcacgcgggc agcgggatgc atatccatat cagtatgcaa
    20941 aataatcgtg gcgagaatgt gctttctgac acagaaggcg aagattcgcc gctgctgaaa
    21001 aagatgctcg ccgggatgat tgacctgatg ccgtcgtcga tggcgttgct ggcaccaaac
    21061 gtgaactcgt atcgccgctt ccagccggga atgtatgtgc cgacgcaggc gtcgtggggc
    21121 cataacaacc gcaccgtcgc cctgcgtatt ccgtgcggcg accgtcataa tcaccgcgtg
    21181 gaatatcgcg tggcgggtgc cgatgccaac ccatatctgg tgatggcagc gatttttgcc
    21241 ggtattttgc atggccttga taacgagctg ccgttgcagg aagaagtcga aggcaacggg
    21301 ctggaacagg aaggcttacc cttcccgatt cgccagagcg atgccctggg tgagtttatc
    21361 gagaatgatc acctgcgccg ctatttaggc aaacggtttt gccatgtgta tcacgcctgt
    21421 aaaaatgatg agctgttgca gtttgagcgc ctcatcacag aaacagaaat tgagtggatg
    21481 ttgaaaaacg cgtaattccc cttgcggggt tgtttcatcg gcctgagcgg agccaggcct
    21541 gacatttgct ggtaatccag acatttcccg gatgtcgcgt tatggagcgc aggcggcaac
    21601 gggcttccgt taacgattaa gactgtgacg aggaaatgag atgatgccga tgattaaatc
    21661 cccgcatggc gagggaggct gcgtatgcgc ccctcctgct actgactgga ctccgccccc
    21721 tttgcttccc ttgctaaaca ggtttgattt caggtcgaca cgaccgcaaa ccttattacg
    21781 caggggaggc agcaattatg gctattaatt caccactgaa tattgctgcg caacccggca
    21841 aaacccgtct gcgaaaatca ctgaaattgt ggcaggtggt aatgatgggt ctggcctatc
    21901 tcacgcagat gaccgtattt gatacttttg gtattgtctc cggcattagc gacggtcacg
    21961 ttccggcgtc ctatttgctg gcgctggcgg gcgtgctgtt taccgctatc agctacggca
    22021 aactggttcg ccagtttccg gaggccggtt cggcctatac ctacgcgcaa aagtcgatta
    22081 acccgcacgt cggatttatg gtcggctggt catcactgct ggattatctc tttttgccaa
    22141 tgatcaacgt tttgttggcg aaaatctatc tctccgccct cttcccggaa gtgccgccgt
    22201 gggtgtgggt ggtaaccttc gtcgccattt taaccgccgc gaatctgaag agcgtcaacc
    22261 tggtcgctaa cttcaatacc ctgtttgtac tggtgcaaat ctccatcatg gtggtgttta
    22321 tcttcctggt ggttcaggga ctgcataaag gagaaggcgt tggcaccgtc tggtcacttc
    22381 agccgtttat cagcgagaac gcgcacctga tcccgattat taccggtgcg acgattgtct
    22441 gtttctcgtt cctcggtttc gatgcggtga ccacgctttc ggaagagacg ccggacgccg
    22501 cacgcgtgat cccgaaagcc atcttcctga cggcggtata cggcggcgtt atctttatcg
    22561 cggcgtcgtt ctttatgcag ctgttctttc ccgatatcag ccgctttaaa gacccggacg
    22621 ccgcactgcc tgaaattgcg ctgtacgtcg gcggcaagct ctttcagtcg attttcctct
    22681 gcaccacgtt tgtgaacaca ttagcgtctg gcctggcttc acatgccagc gtgtcgcgtc
    22741 tgctgtatgt gatggggcgt gacaatgtgt ttccggagcg cgtgtttggc tatgtgcacc
    22801 caaaatggcg gactccggca ctcaacgtca ttatggtcgg gattgtcgcg ctgtcggcgc
    22861 tgttctttga tttagtcacc gcgacagcac tgattaactt cggtgctctg gtggcgttta
    22921 ccttcgtgaa tctgtcggtg tttaatcatt tctggcggcg taaaggaatg aataaaagct
    22981 ggaaggatca cttccactat ttgctgatgc cgctggttgg cgcgctgacg gtgggtgtgc
    23041 tgtgggttaa cctagagtca acgtcactga cactcggtct ggtatgggct tcgttaggcg
    23101 gcgcatattt gtggtatctg atccgccgct atcgcaaagt gccgctgtac gaaggtgaca
    23161 gaacgccggt gagcgaaacg taatagccgg atgcgcggcg cattatcctg cctgagggta
    23221 acgcgccgcg ccattaacta taaaaagatg tataacccgg atcacgagtg ctatcatccg
    23281 cccttatgct tagcgcatta accccagggg caaataatga accacgatat cccgctaaaa
    23341 tattttgata ttgccgatga gtacgcgacc gaatgcgcag agcctgtcgc agaggctgaa
    23401 cgcacaccgc tggcccacta cttccagtta ctgctcaccc gtttgatgaa caatgaagag
    23461 atcagcgaag aagcccaaca tgaaatggct gctgaagcgg ggattaatcc cgtacgcatt
    23521 gatgagatcg cagagttttt gaatcaatgg ggtaatgagt aattatttaa ttgcagacga
    23581 tcggtcagta tcgttgtccg gataaagcgt tcacgccgca tccggctaac aatgacacgc
    23641 actgcagcgt gctttaatgg gtttctcacg atcgtaccgc tctgctcctc gctaagttgt
    23701 tctgtgagtt atagttatag agaaatcacg ggtacgaatg ttgaacagat ggcgacgcca
    23761 cctgttcaac gaccaaccac acggccctca ttacgacccc tgaacacaaa cggtaatggc
    23821 aaatgtcatt aaatattagt aagatagcct ctttacataa aatcccttaa tattatgcgc
    23881 caggtattat cgtctctttt ggtgattgct ggacttgtga gtggtcaggc aatcgccgcg
    23941 cctgaatccc ccccgcatgc tgatatccgc gacagcggtt ttgtctattg cgtcagcggg
    24001 caagtcaaca cctttaaccc atccaaagcg agcagtgggt taattgtcga tacccttgcc
    24061 gcccagtttt atgatcgact gctggatgtc gatccctata cctatcgcct gatgccggaa
    24121 cttgccgaaa gctgggaagt actcgacaac ggcgcgacct atcgcttcca cctgcgtcgc
    24181 gatgttccgt ttcaaaaaac cgactggttt actcccactc gtaaaatgaa tgccgacgat
    24241 gtggtgttta ccttccagcg aatttttgac cgcaacaacc cgtggcataa cgtcaacggc
    24301 agcaacttcc cctacttcga cagcctgcaa tttgccgata acgtgaaaag cgtccgcaaa
    24361 ctggataatc ataccgttga gttccgactc gcccagccgg atgcttcttt tttgtggcac
    24421 ctcgcaaccc attatgcttc ggtcatgtcg gcagaatatg cccggaagtt agagaaagaa
    24481 gatcgccagg agcaactcga ccgtcaaccg gtcggcaccg gtccgtatca gttgtcggaa
    24541 taccgcgccg ggcaatttat tcgcctacaa cgtcatgatg acttctggcg cggtaaaccg
    24601 ttaatgccgc aggtagtggt ggatttaggc tccggcggca ccggacgtct gtcgaaactc
    24661 ctgaccgggg aatgcgacgt tctggcctgg cctgctgcca gccagctatc cattttgcgt
    24721 gacgacccgc gcttgcgttt aacgctgcgt cctgggatga acgtcgccta tctggcattt
    24781 aacaccgcca aaccgccgct aaataatccc gctgtccgcc atgcactggc actggcgatt
    24841 aataaccagc gcctgatgca atccatctat tatggtacgg ctgaaacggc ggcctctatt
    24901 ttaccgcgcg cctcgtgggc ctatgacaac gaggctaaaa ttactgaata caatccggcg
    24961 aaatcgcgcg aacagttgaa ggcgttgggg ctggaaaact taacgctgaa actgtgggtg
    25021 cccacacgtt cgcaggcgtg gaaccccagt ccactgaaaa ctgccgaact gattcaggcg
    25081 gatatggcgc aggttggcgt aaaagtggtg attgtgccgg tagaaggtcg ctttcaggag
    25141 gcgcggttga tggatatgag ccatgatctg acattatccg gttgggcgac ggacagtaac
    25201 gacccggaca gtttcttccg tcctttactg agctgcgcgg caattcattc acagaccaac
    25261 ctcgcccact ggtgcgatcc gaaattcgac agcgtgttgc gtaaggcgct ctcctcgcag
    25321 cagctggcgg cgcgtattga agcctatgac gaagcgcaga gtattctggc gcaggaatta
    25381 cctattctgc cgctggcgtc gtcattgcgt ttgcaggcct atcggtacga tatcaaaggt
    25441 ctggtactta gcccgtttgg taacgcctcc tttgctgggg tgtatcgcga gaaacaggat
    25501 gaggtgaaaa aaccatgatt atcttcacct tacgccgcat tttgttattg attgtcacct
    25561 tgttcctgct gaccttcgtt ggcttcagct tgagctattt cacccctcat gcgccgttac
    25621 aaggcgcgtc actgtggaat gcctgggtgt tctggtttaa cggcctgatc cactgggatt
    25681 ttggtgtttc cagcattaac ggtcaaccga ttgccgaaca gttaaaagag gtcttcccgg
    25741 cgacgatgga actgtgcatc ctcgccttcg gttttgcgct gattgttggc atccccgtgg
    25801 ggatgattgc cgggattacg cgccataagt ggcaggataa cttgatcaac gccatcgcct
    25861 tactgggctt ttcaatcccg gtgttctggc tggcgcttct gttgacgctg ttttgttcac
    25921 tcacgctagg ttggttgccc gtttcagggc gtttcgatct gctctacgaa gtgaaaccga
    25981 ttaccggttt tgcgttgatt gatgcctggc tttcggactc accatggcgg gatgaaatga
    26041 tcatgagcgc aatccgccat atgatattgc ccgtgatcac cctgtcggtt gcgccaacaa
    26101 ctgaagtgat ccgcctgatg cgtatcagca ctatcgaagt gtatgaccag aactatgtaa
    26161 aagcggcggc gacccgcggt ttgtcgcgct ttactatttt gcgtcgccac gttctgcata
    26221 acgcgttacc gccggttatt cctcgcctgg gcttacagtt ttccaccatg ctgacgctgg
    26281 cgatgatcac cgaaatggtt tttagctggc cgggtttagg gcgctggtta attaacgcca
    26341 tccgccagca ggattacgca gccatttccg ccggagtgat ggtgtgtggc tcactggtca
    26401 ttattgttaa cgtgatttct gatattttgg gtgccatggc taaccctctg aaacataagg
    26461 aatggtatgc cttacgatag cgtatacagc gaaaagcgcc cgccgggcac gctgcgtacc
    26521 gcctggcgca aattttatag tgacgcctct gcgatggtcg gcctgtacgg ctgcgcgggg
    26581 ctggctgtac tgtgtatttt tggcggctgg tttgcgcctt acggcatcga ccagcaattt
    26641 ctcggttatc aattattgcc gccatcatgg tcgcgctatg gcgaagtttc tttcttcctg
    26701 gggactgacg acctcggacg cgatgtttta agccgtttac tgagcggagc tgcgccaacc
    26761 gtgggtggcg catttgtggt gacgcttgcc gcgacgatct gtggcctggt actgggcacc
    26821 ttcgccgggg cgacacacgg cctccgctca gcggtgctta accatattct ggatacctta
    26881 ctggcgatcc cttcgctgct gttggcaatt atcgttgtag cgtttgccgg accgagtttg
    26941 tcccacgcta tgtttgctgt ctggctggca ttgctgccgc gtatggtacg ttcgatttac
    27001 agcatggtgc atgacgaact ggaaaaagag tacgttatcg ccgcccgtct ggatggcgca
    27061 tcaacgctga atattctctg gtttgctgtg atgccaaaca tcaccgccgg gctggtcacc
    27121 gagatcaccc gcgcactgtc gatggcaatt ctcgatatcg ccgcgctggg ctttctcgat
    27181 ctcggcgcac aactcccctc gcctgaatgg ggagcgatgc tcggtgatgc gctggaactg
    27241 atttatgtcg ccccgtggac tgtcatgctg ccaggtgcgg caattatgat tagcgtgttg
    27301 ttggttaacc tccttggcga tggcgttcgt cgtgcgatta ttgcgggggt ggaataatgc
    27361 cattactcga tattcgtaac ctgaccattg aatttaaaac cggtgatgag tgggttaaag
    27421 ccgtcgaccg ggtaagtatg accttaaccg aaggtgaaat ccgcggtctt gttggtgaat
    27481 ccggttccgg caagagtttg attgcgaaag cgatttgtgg ggtgaataaa gataactggc
    27541 gtgttactgc tgaccgtatg cgttttgatg atatcgattt gctgcgtctc tccgcacgcg
    27601 aacggcgcaa actggttggc cataacgtgt cgatgatttt ccaggaaccg cagtcgtgtc
    27661 ttgacccttc agaacgtgtg ggtcgccagt tgatgcaaaa catcccagcc tggacctata
    27721 aaggccgttg gtggcagcgt tttggctggc gcaaacgccg tgccattgaa ctgctgcacc
    27781 gcgtggggat taaagatcac aaagatgcga tgcgcagttt tccctatgag ttgaccgaag
    27841 gtgaatgtca gaaagtgatg atagccattg cactggcgaa tcaaccgcgt ctgctgattg
    27901 ctgacgaacc gaccaactca atggagccaa caacccaggc gcaaatcttt cgcctgctga
    27961 cgcgtctcaa ccaaaacagc aataccacta ttttgcttat cagccatgac ttacaaatgc
    28021 ttagccaatg ggcggataaa attaacgtgc tttactgcgg tcaaacagtg gaaaccgcgc
    28081 cgagtaagga gttggtgacg atgccacatc atccttatac ccaggcgctg atccgcgcga
    28141 taccagactt cggcagcgcg atgccgcata aaagtcgcct caatacgctg cccggcgcta
    28201 tcccactgct ggaacagtta ccgattggtt gtcgtctggg gccacgttgc ccgtatgcac
    28261 aacgagaatg cattgtgacg ccacgtttga cgggggcgaa aaatcatctc tatgcctgtc
    28321 atttcccgct gaacatggag aaagagtgag atgatcgaaa cgctgcttga agtgcgtaat
    28381 ttaagtaaaa cgttccgcta ccggaccggc tggtttcgtc gtcagaccgt agaagcggta
    28441 aaacccttga gctttacgct acgtgaaggc cagacactgg cgattattgg cgagaatggt
    28501 tcgggtaaat ccacgctggc aaaaatgctg gcgggaatga tagagcctac cagcggcgag
    28561 ttattgattg acgatcatcc actgcatttt ggcgattatt ccttccgcag tcagcgtatt
    28621 cgcatgattt ttcaggatcc ttcgacctcg ttgaatcccc gtcaacgtat ctcgcaaatt
    28681 ctggattttc cactgcgcct gaacaccgac ctggaaccgg aacagcgtcg caaacagatt
    28741 attgaaacga tgcgtatggt tgggctgcta ccggatcacg tcagttacta tccgcatatg
    28801 ttggcacccg gacaaaaaca gcgtctgggt ctggcgcgcg cgctaatatt gcgcccaaaa
    28861 gtcattattg ccgatgaagc cctcgcttca ctggatatgt cgatgcgttc gcagttgatt
    28921 aatctgatgc tggagttaca ggaaaaacag ggcatttcgt atatttatgt tacccagcat
    28981 atcggaatga tgaagcacat tagcgaccag gtgctggtga tgcatcaggg cgaggttgtc
    29041 gagcgaggca gcaccgcgga tgtgctggca tcgccgctgc atgaactcac caaacggctg
    29101 attgccgggc actttggcga agcattgaca gcggatgcgt ggcgtaaaga tcgctaattt
    29161 tctgaatcct ccttccagat tatccacctc attctgacaa gagattaaat cttgtcagaa
    29221 tgaggtgatg gataaaataa aatcaaatgc gcgtgattta agacgcaatc tcactttgca
    29281 ggaacgaaag ctctggcgat atcttcgcag ccgacgtttt ggtgatttca aatttcgccg
    29341 tcaacatccg gtggggagct acattctcga ttttgcttgc tgctcggcgc gcgtagtcgt
    29401 tgagctggat ggtgggcagc atgatttggc agttgcctat gataccaggc gcactagctg
    29461 gcttgagtcg cagggctgga ccgtgctgcg tttctggaat aacgagattg attgtaatga
    29521 ggaggcggtg ccggagatta ttctgcagga actgaaccgc cgatcaccct caccctgaaa
    29581 gggcgagggg gccgaccgag ccgaatagct gttgtggtga aaacatggag acagtgctgg
    29641 agaatattcg gcaaggtctg aaccatccca gccatcgcca taaaagggtt aggggctgta
    29701 tgagcctgtt tgttgctggg gtaacaatat ttgcacaata tggtcccctc gcccctctgg
    29761 ggagagggtt agggtaaggg gaaaagcgcc caccctgccg cagcctgctc cggtcggacc
    29821 tggcaactat agctactcac agccaggttg attataataa ccgtttatct gttcgtactg
    29881 tttactaaaa cgacgaatcg cctgattttc aggcacaaca agcatcaaca ataaggatta
    29941 aagctatggg ttttctttcc ggtaagcgca ttctggtaac cggtgttgcc agcaaactat
    30001 ccatcgccta cggtatcgct caggcgatgc accgcgaagg agctgaactg gcattcacct
    30061 accagaacga caaactgaaa ggccgcgtag aagaatttgc cgctcaattg ggttctgaca
    30121 tcgttctgca gtgcgatgtt gcagaagatg ccagcatcga caccatgttc gctgaactgg
    30181 ggaaagtttg gccgaaattt gacggtttcg tacactctat tggttttgca cctggcgatc
    30241 agctggatgg tgactatgtt aacgccgtta cccgtgaagg cttcaaaatt gcccacgaca
    30301 tcagctccta cagcttcgtt gcaatggcaa aagcttgccg ctccatgctg aatccgggtt
    30361 ctgccctgct gaccctttcc taccttggcg ctgagcgcgc tatcccgaac tacaacgtta
    30421 tgggtctggc aaaagcgtct ctggaagcga acgtgcgcta tatggcgaac gcgatgggtc
    30481 cggaaggtgt gcgtgttaac gccatctctg ctggtccgat ccgtactctg gcggcttccg
    30541 gtatcaaaga cttccgcaaa atgctggctc attgcgaagc cgttaccccg attcgccgta
    30601 ccgttactat tgaagatgtg ggtaactccg cggcattcct gtgctccgat ctctctgccg
    30661 gtatctccgg tgaagtggtc cacgttgacg gcggtttcag catcgctgca atgaacgaac
    30721 tcgaattgaa ataatcattc tgttggtaaa tattgggcgg cgttctgccg cccgttatct
    30781 ctgttatacc tttctgatat ttgttatcgc cgatccgtct ttctcccctt cccgccttgc
    30841 gtcaggataa cgatttcctt tacgaccaag gagcgcccat ggaacaacgc cacatcaccg
    30901 gcaaaagcca ctggtatcat gaaacgcaat ccagtactgc ggagtatgac gttctgcctc
    30961 tggtcccgga agccgcaaag gtcagcgatc cctttctgct cgacgtgatc cttgatgaag
    31021 aaacgctggc tcccttcctt tcatggctgg tccctgcgcg cgttcttgca gtggaattgt
    31081 tccctgacca gcttaccgtg acccgttcac agaccttcac cgcttatgaa cgcttgtcta
    31141 cggccctgac ggttgctcag gtttgcggcg tccagcggtt atgtaactac tattcggcgc
    31201 gacttacgcc gctccccggg cctgattcct ccagggaaag caatcatagg ttggcacaaa
    31261 tcacgcaata tgcccgccaa ctggctagct cgccttctat tatcgacaac cgatcgcgcc
    31321 agcatctgaa tgacgtcggt cttactgcct gggactgtgt gatcattaac caaatcattg
    31381 gttttattgg ctttcaggcg cggaccattg cgacatttca ggcttatctc gggcacccgg
    31441 tacgctggtt acccggtctg gaaatacaaa actacgccga cgcgtcactg tttgctgatg
    31501 aatcaatacg ctggcgaagc agctatgagg tggaaaaact acctgaagag tacacaaaaa
    31561 gttcaacagc agaactttgc caactggccg aaacactctc tctccaccct atttcacttt
    31621 cccttctcga aaggttgtta aacagtacac gggttaatac acagccggat aataagcttg
    31681 cggcgttgtt atgcgcgcgg ataaatggca gtcctgcttg ttttgccgcc tgtatggatt
    31741 catcaaatga atataaaaaa tcagccccct tctgcgcaag ggcgaaaatg aaattaacca
    31801 atgggctgac cgtcattctg ttgagcacgc taccgttcag gcgatacaat ggctgacccg
    31861 agcacccgat cgctttagcg ccgcccagtt cagcccttta ctcggacacg aaaaatcatc
    31921 aacgcagatt attaatctgc tggtatggag cgggctgtgt ggctggataa atcgcttaaa
    31981 aatcgcgttg ggtgagacat attaaccttg ccgcgtcaga cagattcgcg taaaactgtc
    32041 agccgctcta atggccacca aaatagacaa ttatgtttca ggacaacccg ctgctagcgc
    32101 agcttaaaca gcaactgcat tcccagacgc cacgcgctga aggggtggta aaagccacag
    32161 aaaaaggctt tggcttcctg gaagtcgacg cgaaaaaaag ttatttcatt ccgccgccgc
    32221 agatgaaaaa agtcatgcat ggcgaccgaa ttatcgcggt gatccacagt gaaaaagaac
    32281 gtgaatccgc agagccagaa gaactggttg aaccgttcct gactcgtttc gtgggtaaag
    32341 ttcagggcaa aaatgaccgt ctggccatcg ttcctgatca tccactctta aaagacgcca
    32401 ttccttgccg cgcagcccgt ggcctgaacc acgagtttaa agaaggcgac tgggcggttg
    32461 ccgaaatgcg ccgtcatccg ctgaaaggcg atcgttcttt ctatgctgaa ctgacacaat
    32521 acatcacttt tggtgacgac cactttgtac cgtggtgggt tacccttgcg cgccataatc
    32581 tggaaaaaga agcaccagac ggcgtcgcta ccgaaatgct cgatgaaggt ctggttcgtg
    32641 aagatctgac cgcgctggat tttgtcacca tcgacagtgc cagcacagaa gatatggatg
    32701 acgccctttt cgctaaggcg ttgccggatg acaaacttca gctgattgtg gcgattgccg
    32761 atccaaccgc gtggattgct gaaggcagca agctggacaa agccgcgaaa attcgcgcat
    32821 tcaccaacta tctgcctggc ttcaacatcc ctatgcttcc tcgcgagctt tctgacgatc
    32881 tctgctcact gcgcgccaat gaagtccgcc cggtactggc atgccgcatg acgctctccg
    32941 ctgatggcac cattgaagat aatatcgaat tctttgccgc caccatcgaa tccaaagcga
    33001 agctggtgta tgaccaggtt tctgactggc tggaaaatac cggtgactgg cagcctgaaa
    33061 gtgaagcaat tgccgaacaa gtccgtttgc tagcgcaaat ttgccaacgc cgcggcgagt
    33121 ggcgtcataa ccacgcactg gtgtttaaag atcgcccgga ttaccgcttt attctcggtg
    33181 aaaaaggtga agtgctggat atcgtcgccg agcctcgtcg cattgccaac cgtatcgtcg
    33241 aagaagcgat gattgccgct aacatttgtg cggcccgcgt tctgcgcgat aagctcggtt
    33301 ttggcatcta taacgtgcat atgggctttg atccggcgaa tgccgacgcg ctggcagcgt
    33361 tgctgaaaac gcacggtctg catgtcgatg ccgaagaagt gctcacgctg gacggtttct
    33421 gcaaactgcg tcgtgaactg gatgcgcaac caactggttt cctcgacagc cgcattcgtc
    33481 gcttccagtc atttgctgaa attagcactg aacccggtcc tcactttggc ctcggtctgg
    33541 aagcatacgc cacctggact tcgccgatcc gtaaatatgg cgacatgatc aaccaccgtc
    33601 tgctgaaagc ggttatcaaa ggcgaaactg cgacgcgtcc gcaggatgaa atcactgtcc
    33661 aaatggccga acgtcgccgt ctcaaccgga tggcagaacg tgatgttggc gactggttat
    33721 acgcacgctt cctgaaagat aaagccggga ccggcacccg tttcgcggcg gaaattgtcg
    33781 atatcagccg tggcggcatg cgtgtgcgtt tggttgataa cggcgctatc gcctttattc
    33841 cggcaccttt cttacacgct gtgcgcgatg aactggtttg cagccaggaa aacggcactg
    33901 tacaaattaa aggtgaaacg gtttacaaag taactgacgt tattgacgtc accattgccg
    33961 aagtccgcat ggaaacccgc ggcattattg cgcgcccggt cgcgtaatct cctttcacgg
    34021 cccattcctc atggatgggc cgtttatttc cccgctctac cttcgttata tccaggcaga
    34081 ttttttaact gactttcgtt tgaaaactgg cgtttttcca aaactggatt acaaataaat
    34141 atataaaaaa acaatgcgtt tagccgccgt taatttcatg taagtcatgt tgttcctttt
    34201 atttaaccgc ggactccgca gttaaccgga ggatatgcat catgaaaacc gttagggagt
    34261 ccacaacgtt gtacaacttt ctcggatcgc acaatccata ctggcggttg acggaaagca
    34321 gcgatgtttt gcgctttttt accaccgaaa ccacagaacc tgatcgtact ttgcagttat
    34381 ctgccgaaca ggctgctcgc atcagggaaa tgacggtcat cacctccagc ctgatgatga
    34441 gtctgaccgt cgatgagagc gatctttctg tgcatctggt aggacgaaaa atcaataaac
    34501 gggaatgggc tggcaacgcg tctgcatggc atgacacacc ggcagttgct cgtgatttat
    34561 cacacgggct ttcctttgct gagcaggtag tttctgaagc acattccgca atagtgattc
    34621 tcgacagccg ggggaatatc caacgcttca atcggttatg tgaagattac actgggttga
    34681 aagaacacga cgtcattggc caaagcgtgt ttaaactgtt tatgagccgt cgtgaagctg
    34741 cggcatccag gcgcaataac cgtgtatttt ttcgaagcgg caatgcatat gaagtcgaac
    34801 tgtggatacc aacacgtaaa ggccagcggc tgtttctgtt tcgcaataaa ataaatttgt
    34861 ccacagcggc agtggcaaaa acgagatttt tttaatctgt tccggtaccg acattaccga
    34921 agagcgccgc gctcaggagc gactgcgtat tctggcaaat accgacagta tcaccggact
    34981 gccgaatcgt aacgcaatgc aggagttaat cgatcacgct attaatcagg ctgataacaa
    35041 taaagttggg gttgtgtatc ttgatttgga taattttaaa aaggtcaacg acgcctatgg
    35101 gcatttgttt ggtgaccagt tattacgcga cgtgtcattg gctattttaa gctgtctcga
    35161 acatgaccag gtgttggcgc gtccaggtgg cgatgagttt ctggtactgg catccaatac
    35221 ctcacaaagc gcgctggaag caatggcatc acgaattttg acccgcttac gcctcccctt
    35281 tcgtattggt ttaattgaag tttataccag ctgttcagta ggtatttcgc tctctcccga
    35341 acatggttcg gacagcgcga ctattattcg tcacgccgac acagcaatgt acacagcgaa
    35401 ggaaggcgga cgaggacaat tttgcgtttt tactccagaa atgaatcagc gggtatttga
    35461 atatctctgg ctggatacca acttgcgtaa agcactggaa aacgatcagt tggttattca
    35521 ctatcaaccg aaaatcacct ggcgtggcga agtgcgcagt ctggaagcac tagtacgttg
    35581 gcagtcacct gaacgtgggt tgattccacc gttggacttc atttcctacg ccgaagagtc
    35641 agggctaatt gtgcctttag gccgttgggt gattctcgat gtcgtacgcc aggtggcaaa
    35701 gtggcgggat aaaggcataa acctgcgagt ggcggtaaat atttctgcac gtcagctcgc
    35761 cgatcaaacc attttcaccg ccctgaaaca ggttctccag gaactcaatt ttgaatactg
    35821 ccctatagat gttgaactga cagagagttg tctgattgag aatgatgaac tggcactgtc
    35881 tgttattcaa caatttagcc aactaggtgc gcaagtgcat ctggatgatt ttggtaccgg
    35941 ctactcttca ctttcgcaac tggcgcgctt tccgatcgat gccatcaaac ttgaccaggt
    36001 ttttgttcga gatattcaca aacaacctgt ctcgcagtca ctggtccggg cgatcgtcgc
    36061 tgtggcccag gcattgaatc ttcaggtgat cgccgaaggt gtagagagtg caaaggaaga
    36121 tgctttttta accaagaacg ggatcaatga gcggcaagga tttttgtttg ccaaaccgat
    36181 gcccgccgtc gccttcgaac gctggtataa acgctatctg aagcgcgcat aagtagatga
    36241 atatatatac ttagcgctat atcccgtcat tatatatcgt attcctggat gataattagc
    36301 cgttgctgtt aaacacttct cttttagcgg caatttttgc attattcatt ttccactttt
    36361 gctataagga tgtccttgat gtctgaattt gatgcccagc gcgttgccga acgtattgat
    36421 attgtgttag atatccttgt ggcaggcgat taccactctg cgatccataa tctcgagata
    36481 cttaaagcag aactcttgcg ccaggttgct gaatctacgc cagacatccc gaaggcaccg
    36541 tgggagattt aacaatcgtg ccttcaggtt ttcctatttg aggatgcccg gcgcgtcagt
    36601 atttttttat ttagtattat aacgttataa gaattacagc gatgaattcc cgacaacaaa
    36661 ctattctaca gatggtcatt gaccagggtc aggttagcgt aaccgatctg gcaaaagcca
    36721 ctggagtttc tgaagttacc attcgccagg atctcaacac cctcgaaaaa ctgagttacc
    36781 tccgccgtgc acatggcttt gcagtttcgc ttgatagtga tgacgtcgaa acccgtatga
    36841 tgagcaacta tacgctgaag cgtgaactcg ccgagtttgc cgcgtcactg gttcaaccgg
    36901 gcgaaaccat ctttatcgaa aatggcagca gcaatgccct gctcgcccgg actctggggg
    36961 agcagaagaa aaatgtcact atcatcacgg tcagcagcta catcgcgcat ttgctgaaag
    37021 acgcgccttg tgaagtcatt ttgctcggtg gcgtgtacca gaaaaaaagc gaaagtatgg
    37081 ttggcccgtt gacacgccag tgcatccaac aggtgcattt cagcaaagca tttattggta
    37141 ttgatggctg gcaacctgaa actggattca ccggtcgcga tatgatgcgt accgatgtgg
    37201 tcaatgccgt gctggaaaaa gagtgcgaag cgatagtcct gactgacagc tcgaaatttg
    37261 gtgctgtaca ttcatactcc atcggtcccg ttgagcgatt caatcgcgtg attaccgatt
    37321 cgaaaatacg cgccagcgat ctgatgcatc ttgagcacag taaactcacc gttcacgtcg
    37381 ttgacattta accatttccc ttctctacgg atgatttgca gtttggcaaa tcatccgctc
    37441 taagatgatt cctggttgat aattaagact atttacctgt tattaacacg cttaagatat
    37501 aaaattatta tcagcgatat aacaggaagt cattatcacc tgcgtgatat aaccctgcgc
    37561 gcgagcagat ttcacggaat aatttcacca ggcttattct tagctattat agttatagag
    37621 agcttacttc cgtgaatcat aaattcagga gagagtatta tgtttgtaac gagcaaaaaa
    37681 atgaccgcgg ctgttctggc aattactttg gcaatgtctc tgagtgcctg ttctaactgg
    37741 tctaaacggg accgcaacac cgcaatcggc gcgggtgcag gggcattagg cggtgcagta
    37801 ctgaccgatg gcagtacgtt gggtacatta ggtggtgcag ctgtcggtgg tgttattggt
    37861 caccaggtcg gtaaataatc gtaaataaat gtcgattatc gactcgcaat tataatacgc
    37921 ttgattctgt tcagtacgaa ataactgaat aatacgcaca gtaaaatatt cgaaagccac
    37981 ggatatatcc gtggcttttt atgttaaccg cctgcgagtt ttaccttcat ccctttcgct
    38041 tcaagtagtg attttaataa atcacgctta tcgccctgga tttcaataac tccatcttta
    38101 actgctccgc cgcagccgca ttttttcttc agttccgctg caagttttgt cagttcggca
    38161 tcatcgagat cgacaccggt aatcaggcaa acgcccttac ctttacgtcc actggtctga
    38221 cgctgaatac gcaccacacc gtcgccttta gggcgtacag gggccgcttt gggctcatca
    38281 atacgtccgg tttccgttga gtagaccaga cggctgttgg aatcactcat catgcactcc
    38341 gctgtaaaga ggcgttgatc gctttcagcg tctgcgctgg atctaccgat tgcgttaccg
    38401 ggcgaccaat caccatataa tcaacaccag ccgacaacgc ctgttctggc gtcataatgc
    38461 ggcgctggtc accagcttca ctcccctgcg gacgaatgcc cggcgtaacc agtttgaact
    38521 cctgaccgaa tacctgttta aagcgcacag cttcctgagc agaacacacc acaccatcaa
    38581 ggccacattt ttgcgtcagt gccgccagac gttctgcata atctgcaggt gacagtgtca
    38641 tgccaagatc gaccaggtcg ctggcttcca tgctggtcaa cactgtcaca gcaatcaaaa
    38701 gcggtgcatc tttgccaaac ggaaccagtg cctcacgcgc tgcggtcatc atacgcgccc
    38761 caccagaggc atgaacattc accatccaca cgcctaagtc agctgcagca gcgacagcgt
    38821 gcgctgcagt gttaggaata tcgtggaatt tcaggtcaag aaagatatca aaaccacgct
    38881 gttgaagttc gcgcacaaac tgtggcccaa acaatgtaaa catctctttg ccgaccttca
    38941 gacgacaatc gcgtgggtcg atcttgtcga caaaggacag cgcgtcatca cgattatgat
    39001 aatcaagggc aacaaccaca ggagaattcg taacagcgcg ggaagaagat gaagcagtta
    39061 acgtcatgac cagaccttct tgatgatggg caccggaagt ggcgcgaaac aggtaaacgg
    39121 cgagcattct acccgtggaa ataaaaaatt aacagttgcg atttttcccc tggcaggctt
    39181 tttctgctcc gtcgttgccg gagcacaatg gaacatcaat aactagtatg ttgtaactaa
    39241 agtcggattt ttttaaaaat tacaggccat caagaccgcg aatcggttta atggttgacc
    39301 aggcccgaca agacggacaa tgccagtaga gggtgtatgc ggtaaaacca catttctggc
    39361 agcgataacg aggcttacta cgtacctttt cgccaaccat gtcacgcagc accatcaggc
    39421 tctctttggc acgcccttct tccgcttcat ttaagtggta atccattaac ttatggaaca
    39481 cacgcatggt cggatgacgc tgaagctggc gcgtaatata gacctgtgcg gcctcactac
    39541 cgtcgcgcgc ttcgatgata tcagcaagca tcaattcagc atcggcaccg gtgttctctt
    39601 ccaccgcgcg ttgcaggaat tctgcccatt cggcagtttt acccaactgc tgatagcagg
    39661 tttgcaacat ttccagcgtt tcgctgacca gttctctgtc ctgggatatc acgcgttgca
    39721 aactttcgac ggctttggcg tattctcctt tcgccataaa cacgcgtccc atcatgatgg
    39781 atacgcgggc gctgttttta tctgccgccg cccctttttt tagcaaggtc atggcacgat
    39841 cgagatcgtc gctggccata tgctgcaggg ctaactcaca gtagaaatgg gcaatttcga
    39901 cgcgctgttt atctttaccc agcttcacca ggcgttcggc aacatcaatt gctttctgcc
    39961 actcgctggt agcctggtag atttgtagca actgttgcag cgcgccaatg cggaagtcag
    40021 tttcatcggt cagctgattg aacatgtctt ccgcgcggtc gtataacccg gcggccatgt
    40081 aatcacgccc cagttgttga atcgccaaca gacgctgttc ataggtcagt gaggcgcttt
    40141 ccattagggt ctgatggatg cgaatagcgc gatcaacttc gccacgcgaa cggaacaggt
    40201 ttccgagcgt aaggtgggct tcaacggtgc ctgtatcctc tttaagcata tcgagaaaca
    40261 ggtctaccgc tttatcctgt tgattactaa gcaggaagtt aacccccgct acgtaatcac
    40321 gcgacaagcg gttggcttca tcttgcttgt tttgttgcgc acttctgcgg cccatatacc
    40381 agccataggc agcggctaca ggcaaaagca gaaacaacaa ctccagcata gaaagttatt
    40441 ccttcgccgc tgacgagtgc ggcactacag ccacgtcagt cgcgggtgaa agctggtttt
    40501 ccagtcgctt tattttacgt tcagcgcgcg ccagggaaac acgaactcgc agccagaaca
    40561 ggccacaaat caaccaaccg atagcaaccc ccgcagcaaa caataccgcc agcaatgtgg
    40621 aaatacggta ctccccttgc gctaacagat aattaaacgt cacctgttga tcgttctgcg
    40681 cacccaacgt gaccgaaatc acgaagatcg ctaacaccag taagaaaatg agtaaatatt
    40741 tcacattact tcccgttatg tggttaaagc gaataaatta tggtcaactt accgcaatag
    40801 ccctttaaag taccattttc gcgccgtctg cgaaatggca tccacgcgca ttgttgatga
    40861 tttaagggct aaattcggaa aatcaaccag cgttaacttt cttgttctcg ttgcgctatt
    40921 tcgcgatttt cttccgcagg tggtgttaat ggcccacaaa ttcgttgcgc aagccacgtt
    40981 gccaccgcca ccagcgccca cgaaatcaac gtagctacta ccagatcgcg tggccaatgc
    41041 atcccgagca gcaggcggct tcccatgact cccgttgccc agaccagcaa gatagcaatg
    41101 gttaacgttc gccgacgcgg ccacagcaaa ccaacggcca gcagtgccca actggcagca
    41161 aacatcgtgt gaccggaagg aaaggcaaac cccgtctctt tctgccagtg tgaacgcaaa
    41221 tattgtggga tatttttctc ttcagccaac tgttctttca ctagatttcc gcgttctgct
    41281 cgctttaaag tgtagaactc atcaaccgga atatgatgtg ttttttccag ccagataaca
    41341 aaaggtcgtg gttcctggac tttgtctttg atccaggatt taacgccttg tcccacaatg
    41401 attacggccg ccagaatggc aaataataca aaggcagcct taatgcaaaa acgcagacac
    41461 cagagaaacc agccgaataa aatcaaatgt gtaatgacgc cccagggctg ggtgacagtt
    41521 tcagtaaccc aaaaagccgc ttttagtagc caactttgtt ctccaggttg ccaacgccag
    41581 ccagaaatcc atacggctac tggcatgaca agcaatagtg cagctcccac tgcggtacgt
    41641 ctggcaatcg aacgcatggc ctctcctttt tgataagtcc cacaatcata actgaaaacg
    41701 ccagttccag gaaaaattga cagatttgtg ccattccgtg aacgatcgac gcgtcgtgat
    41761 taggtgaacc ccttctcgtt atggcaaaat aagccaatac agaaccagca ttatctggag
    41821 aatttcatgc agcttaaacg tgtggcagaa gccaaactgc caaccccatg gggcgatttc
    41881 ctgatggtgg gatttgaaga actggcaacc ggacacgatc atgtcgcgct agtctatggc
    41941 gatatttccg ggcatacccc ggtacttgcg cgcgtccatt ccgaatgtct gaccggtgac
    42001 gccctgttca gcttgcgctg cgattgtggc ttccagctcg aagcggcatt gacgcaaatt
    42061 gccgaggaag gccgtggtat tttgctgtat caccgtcagg aaggtcgtaa cattggtctg
    42121 ctgaataaaa tccgcgctta cgcactgcag gatcaaggtt acgataccgt agaggctaac
    42181 catcagttag gcttcgccgc tgatgagcgc gacttcactc tttgcgctga tatgttcaaa
    42241 ctccttggcg tcaatgaagt ccgcttgtta accaataacc cgaaaaaagt cgaaattctg
    42301 accgaagcag ggattaatat tgttgaacgc gtaccattga ttgtaggtcg taaccccaat
    42361 aacgaacatt atctcgatac caaagccgag aaaatgggtc atttgctgaa caaataaccc
    42421 tctcgcattg tggaattaat ttgcttgccg gaagcaaaat aaccggcaag caaatatttg
    42481 ttacttcagc atattacgaa tgacataatg caaaatgccg tcgttctggt agtaggtcaa
    42541 ctccgtcgcg gtgtcgatac gacaacggca gggtacgacc tcctggctac catccgcgcg
    42601 cgtaagcgtc accggaaccg tcgcaccggg ttgtagattt tgcagatcgc caatatcaat
    42661 cttctcttcc ccggttagtc ctaacgtttt acgcgttacg ccttgcggaa attccagcgg
    42721 caggatgccc atgccaatta aattcgaacg gtgaattcgt tcaaacgatt cggcaatcac
    42781 cacacgaatt ccaagcagac gaggaccttt tgccgcccag tcacggcttg agcctgaacc
    42841 atactctttc ccggcaatca ccgccaacgg cgtttgttcc tgcttatagc gcatcgcagc
    42901 atcataaata gagactacgt cgctgtcagg taaatgccgc gtcatccccc cttcaacgcc
    42961 aggcaccatt tcattacgga tgcgaatatt ggcgaaggtg ccgcgcatca tcacttcatg
    43021 gttaccacgc cgcgaaccgt aggagttaaa gtcttttcgc tcaacacccc gaccttgtag
    43081 atatcgaccc gctgggctgt cgggcttaat actgcccgcc ggagagatat ggtcagtggt
    43141 gactgaatcc cccagcattg cgaggatccg cgcaccgtga atatcttcca ctggtgctgg
    43201 tgttgcctgc atttcatcaa agaaaggcga taagcgaata taggttgagt cctcctgcca
    43261 accgtaggta tcggatcgtg tgacgttaat ttccttccac tctgctgtgc cttcaaaaac
    43321 ttctgcgtac tctttgcgga acatttctgt ggagacttgt tctaccgcac gggcaatttc
    43381 ttgtgccgat ggccagatat ctttcagata aaccggatcg cctttacgat catgaccaat
    43441 aggctcagaa gccaggttga tattcatatt tcccgccagc gcataggcaa ccaccagcgg
    43501 cggcgaggcc agccagttgg ttttaaccag cggatggata cggccttcaa agttacggtt
    43561 gccggacagc actgcaccga cggttaaatc gccttttttg attgccgttt cgataggatc
    43621 gggcagcggc ccagagttac caatacaggt agtacaaccg tatcccacaa ggttaaaccc
    43681 cagttcgtcg agaaacggtg tcagtttcgc ttttgccaga tagtcagaaa caactttcga
    43741 gcctggtgcc agcgacgctt tgacccacgg ttgccgcttg aggccaagaa ttacggcttt
    43801 tttcgccagc aagcctgcag ccatcagcac acttgggtta gaggtgttgg tgcacgaggt
    43861 tatcgcagca atgaccacag cgccatcagg taactgatac tgatgtccgt tcataacata
    43921 atcgaccggc tggcgatctt tatgcgtggc attcacttcc agttcgttac tggcggcaaa
    43981 tacttttggt acatcgggca gtgcaacgcg atcctgtggg cgttttggcc ctgccaggct
    44041 cgcttcaacg tcattcatat ccagttctaa cgtactggta aaaatcggtt catcgcccgg
    44101 gttacgccac atgccctgcg ctttggcata tttttcgacc aactcaacct gatcttcgct
    44161 gcgcccgctt aaacgcatgt aatcgagggt tacagcatcg attgggaaga agccacaggt
    44221 ggcaccatat tctggcgaca tattggcaat ggtggcgcga tccgccagcg gtagtgaatc
    44281 cagaccatca ccataaaatt cgacgaattt ctccaccacg ccatgtttgc gcagcatttg
    44341 ggtaacagtg agaaccaggt ctgtggcggt aataccttca cgtaatttac cggtaagttt
    44401 aaagcccact acgtccggga taagcatgga aaccggctgg cctaacat
//
LOCUS       DAEEKQ010000013        43713 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.013, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000013 DAEEKQ010000000
VERSION     DAEEKQ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43713
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.013"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..559)
                     /locus_tag="KLK57_000754"
     CDS             complement(<1..559)
                     /locus_tag="KLK57_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBE0891213.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPDTARDFLAFHLPAPIHALCDMKT
                     LKLESSSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVLLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQR"
     gene            complement(762..998)
                     /gene="feoC"
                     /locus_tag="KLK57_000755"
     CDS             complement(762..998)
                     /gene="feoC"
                     /locus_tag="KLK57_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HBE0891214.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(998..3319)
                     /gene="feoB"
                     /locus_tag="KLK57_000756"
     CDS             complement(998..3319)
                     /gene="feoB"
                     /locus_tag="KLK57_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HBE0891215.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTSARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="KLK57_000757"
     CDS             complement(3336..3563)
                     /gene="feoA"
                     /locus_tag="KLK57_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HBE0891216.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(4001..6322)
                     /locus_tag="KLK57_000758"
     CDS             complement(4001..6322)
                     /locus_tag="KLK57_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HBE0891217.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYIDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKVAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFKNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(6419..6895)
                     /gene="greB"
                     /locus_tag="KLK57_000759"
     CDS             complement(6419..6895)
                     /gene="greB"
                     /locus_tag="KLK57_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HBE0891218.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCMENLKIVNYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            7123..7842
                     /gene="ompR"
                     /locus_tag="KLK57_000760"
     CDS             7123..7842
                     /gene="ompR"
                     /locus_tag="KLK57_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HBE0891219.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            7839..9191
                     /gene="envZ"
                     /locus_tag="KLK57_000761"
     CDS             7839..9191
                     /gene="envZ"
                     /locus_tag="KLK57_000761"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HBE0891220.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="KLK57_000762"
     CDS             complement(9267..10889)
                     /gene="pckA"
                     /locus_tag="KLK57_000762"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HBE0891221.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            11268..11582
                     /locus_tag="KLK57_000763"
     CDS             11268..11582
                     /locus_tag="KLK57_000763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBE0891222.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNPWRAEKAIWD
                     FG"
     gene            11595..12968
                     /locus_tag="KLK57_000764"
     CDS             11595..12968
                     /locus_tag="KLK57_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HBE0891223.1"
                     /translation="MAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLSNG
                     LTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALVVILARTQSRLIDSIQ
                     KLFTLIATGLLPLVSLLTLMFIITLPFTDLSAISRHISAAGLLLTLAFLQLILMAIVR
                     DPQKASLPWTGPLRCLIKTALLVAPLYVFVAAWALWLRVAQYGWTVDRLQGALAVLVL
                     LVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMA
                     RYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQ
                     EQVSEKVLAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGQA
                     ERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLDLTVDGET
                     LEINLSK"
     gene            complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="KLK57_000765"
     CDS             complement(13103..13981)
                     /gene="hslO"
                     /locus_tag="KLK57_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HBE0891224.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="KLK57_000766"
     CDS             complement(14006..14407)
                     /gene="hslR"
                     /locus_tag="KLK57_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HBE0891225.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="KLK57_000767"
     CDS             complement(14418..15086)
                     /gene="yrfG"
                     /locus_tag="KLK57_000767"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HBE0891226.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="KLK57_000768"
     CDS             complement(15151..17286)
                     /gene="igaA"
                     /locus_tag="KLK57_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HBE0891227.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIYTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDGSRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            17606..18166
                     /gene="nudE"
                     /locus_tag="KLK57_000769"
     CDS             17606..18166
                     /gene="nudE"
                     /locus_tag="KLK57_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HBE0891228.1"
                     /translation="MSKSLQKPTILNVETVAHSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="KLK57_000770"
     CDS             complement(18331..20883)
                     /gene="mrcA"
                     /locus_tag="KLK57_000770"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HBE0891229.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQEATAMLADGSTVALSMDGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGVGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            21003..21782
                     /gene="hofM"
                     /locus_tag="KLK57_000771"
     CDS             21003..21782
                     /gene="hofM"
                     /locus_tag="KLK57_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HBE0891230.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQRLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRCRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            21782..22321
                     /gene="hofN"
                     /locus_tag="KLK57_000772"
                     /pseudo
     CDS             21782..22321
                     /gene="hofN"
                     /locus_tag="KLK57_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312263.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
     gene            22305..22745
                     /gene="hofO"
                     /locus_tag="KLK57_000773"
     CDS             22305..22745
                     /gene="hofO"
                     /locus_tag="KLK57_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HBE0891231.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWATLYRLVDTTPFSEEKTLPFSPLDFQLPGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            22735..23139
                     /gene="hofP"
                     /locus_tag="KLK57_000774"
     CDS             22735..23139
                     /gene="hofP"
                     /locus_tag="KLK57_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HBE0891232.1"
                     /translation="MRVKRWLLAGIVLCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGERIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDVLTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            23051..24289
                     /gene="hofQ"
                     /locus_tag="KLK57_000775"
     CDS             23051..24289
                     /gene="hofQ"
                     /locus_tag="KLK57_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HBE0891233.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            24690..25211
                     /gene="aroK"
                     /locus_tag="KLK57_000776"
     CDS             24690..25211
                     /gene="aroK"
                     /locus_tag="KLK57_000776"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HBE0891234.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            25268..26356
                     /gene="aroB"
                     /locus_tag="KLK57_000777"
     CDS             25268..26356
                     /gene="aroB"
                     /locus_tag="KLK57_000777"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HBE0891235.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            26448..27734
                     /gene="damX"
                     /locus_tag="KLK57_000778"
     CDS             26448..27734
                     /gene="damX"
                     /locus_tag="KLK57_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HBE0891236.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTSSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPAAPVASTKAPTATSTPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETARNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            27841..28677
                     /gene="dam"
                     /locus_tag="KLK57_000779"
     CDS             27841..28677
                     /gene="dam"
                     /locus_tag="KLK57_000779"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBE0891237.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKTYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            28695..29372
                     /gene="rpe"
                     /locus_tag="KLK57_000780"
     CDS             28695..29372
                     /gene="rpe"
                     /locus_tag="KLK57_000780"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HBE0891238.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            29365..30123
                     /gene="gph"
                     /locus_tag="KLK57_000781"
     CDS             29365..30123
                     /gene="gph"
                     /locus_tag="KLK57_000781"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HBE0891239.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATQRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            30116..31120
                     /gene="trpS"
                     /locus_tag="KLK57_000782"
     CDS             30116..31120
                     /gene="trpS"
                     /locus_tag="KLK57_000782"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HBE0891240.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            31280..32185
                     /locus_tag="KLK57_000783"
     CDS             31280..32185
                     /locus_tag="KLK57_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891241.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGLCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            32202..32564
                     /locus_tag="KLK57_000784"
     CDS             32202..32564
                     /locus_tag="KLK57_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HBE0891242.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            32648..33811
                     /locus_tag="KLK57_000785"
                     /pseudo
     CDS             32648..33811
                     /locus_tag="KLK57_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417840.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
     gene            33811..35037
                     /locus_tag="KLK57_000786"
                     /pseudo
     CDS             33811..35037
                     /locus_tag="KLK57_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
     gene            35034..35912
                     /locus_tag="KLK57_000787"
     CDS             35034..35912
                     /locus_tag="KLK57_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HBE0891243.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLGVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            35923..36276
                     /locus_tag="KLK57_000788"
     CDS             35923..36276
                     /locus_tag="KLK57_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HBE0891244.1"
                     /translation="MKKIGVAGLQREQIKKTIEATATGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            36288..37592
                     /locus_tag="KLK57_000789"
     CDS             36288..37592
                     /locus_tag="KLK57_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HBE0891245.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVMRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPIVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            37604..38689
                     /locus_tag="KLK57_000790"
     CDS             37604..38689
                     /locus_tag="KLK57_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HBE0891246.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAALAALLKPGQRLLVHDAPVYP
                     TTQVIIEQMGLTLITADFNDLSALKQVVDEQLPDAALVQHTRQQPQDGYILADVLATL
                     RSAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRS
                     TLYSGGSQIQGAQALEVLCGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRNGEETVLRILRESIASI"
     gene            complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="KLK57_000791"
     CDS             complement(38841..39572)
                     /gene="phoB"
                     /locus_tag="KLK57_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HBE0891247.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKATK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="KLK57_000792"
     CDS             complement(39672..40457)
                     /gene="frlD"
                     /locus_tag="KLK57_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HBE0891248.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TLVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="KLK57_000793"
                     /pseudo
     CDS             complement(40454..41285)
                     /gene="frlC"
                     /locus_tag="KLK57_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026213.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
     gene            complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="KLK57_000794"
     CDS             complement(41335..42357)
                     /gene="frlB"
                     /locus_tag="KLK57_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HBE0891249.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKPLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="KLK57_000795"
     CDS             complement(42378..>43713)
                     /gene="frlA"
                     /locus_tag="KLK57_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
                     /protein_id="HBE0891250.1"
                     /translation="GSQELQRKLGFWAVLAIAVGTTVGSGIFVSVGEVAKAAGTPWLT
                     VLAFVIGGLIVIPQMCVYAELSTAYPENGADYVYLKNAGSRPLAFLSGWASFWANDAP
                     SLSIMALAIVSNLGFLTPIDPLLGKFIAAGLIIAFMLLHLRSVEGGAAFQTLITIAKI
                     IPFTIVIGLGIFWFKAENFAAPTTTAIGATGSFMALLAGISATSWSYTGMASICYMTG
                     EIKNPGKTMPRALIGSCLLVLVLYTLLALVISGLMPFDKLANSETPISDALTWIPALG
                     STAGIFVAITAMIVILGSLSSCVMYQPRLEYAMAKDNLFFKCFGHVHPKYNTPDVSII
                     LQGALGIFFIFVSDLTSLLGYFTLVMCFKNTLTFGSIIWCRKRDDYKPLWRTPAFGLM
                     TTLAIASSLILVASTFVWAPIPGLICAVIVIATGLPAYAFWAKRSRQLNALS"
BASE COUNT        10157 a        11128 c        11988 g        10440 t
ORIGIN      
        1 cgcgttgacg aatatgtttt tgaattaact ccagcagcgc catacgtcgg tgctgcatga
       61 tttcatcatc aggcatgacg gtgacatcaa tcagcggaaa tgcggaggca taaagctgcc
      121 ttgccagttt gggatcggcg aaacaatcca gccaacacag cgaataggga taggggcttt
      181 caataccgtg gtaaaacaac aatagcacca ccatcggcaa cgttttgtat ccagcatcaa
      241 ggtgattttg cattgcggca atagcgtaac gcatcatgcg aaatgcgatc agtttgtttg
      301 aggtgctttg atgttcaatc agacaataga tgtatcctgg tccttgttcc gttttcaccg
      361 accacagcac atcggaatag ctttcacgca gatcgtcatc aataaagctg ctcgactcca
      421 gcttgagggt tttcatatca caaagcgcgt gaatgggtgc cggtaaatga aacgcaagaa
      481 aatcacgagc cgtgtccggt tggcgtaaaa agagtttgaa cagcgcatcg tgtggggtgg
      541 aactctgctt tttgctcatg gcgttccttc acctgttgtt tgaatgccgc gactctacgc
      601 gccactccac acctgtgcat tagcgagttt tcatccctgc gagatgcttt caaaggaact
      661 tacgccgtgc tgcaagagtt gcatttttat ggaaagccgc acgcaaaaaa gcccggatgc
      721 agacgcatac cgggctttcc aaaacaggcg atggagtaag gttaacgcag cgcccaccac
      781 tcgcgcagac aggcttttcc ttccgggcag cttttacaac tgccagagag gcagccgtca
      841 ggttcttcct gaatccgcac ggctttgccc atactttcca gttgttgcag catggcgtta
      901 atcattggct gcggagtgtt caatgtctgg cttatctggg tcgcttccat acggccccgt
      961 aacgccagca aatcacgtac ctgaataagt gaagccatta atggcaatca ccggtggtgc
     1021 tggctgcgca gcaactgctt accgacttgc gggttgccag cagttcgata tccacccggc
     1081 tacgcgcgcg gcgcagcaga ccgataacta cgatgttaaa caggattacc gccagaatgc
     1141 acaccaggct gtaagttggg tgctgactgt agctggcgac ctgatagaac aatgttgcca
     1201 gtgagtaagc gatattcagc ccccacagga tggagaagcc catccagcca cggcttgatt
     1261 cacgggcgat ggctcccatc accgagatac atggtacata cagcaggacg aaaatcaggt
     1321 agctgtatgc ggcagctgcg ctaccgaatt tctgatccat cacgcccatc gccccggtac
     1381 ccatttcacc gtcgcctttg ctagcttcaa tggggttcat cagtacgcta aggctgaagg
     1441 tgtctttcag gctctgccag gtttcatcta ccgcactgaa cagctcttca ccgaggttga
     1501 attccgccgg attgaactct tcgtcctgaa tattttctgc ggtgtagagg gtgttgagcg
     1561 tacccaccac cacttctttt gccattgcgc ccgtaaacag gccaaccgtt gcctgccagt
     1621 tatcttcatg tacgccaatc ggcttgaaga caggggttat cacccggctg acggacgcca
     1681 gcgccgagtc gttgatgtta tcgacgattt tcccgctcaa cgagaagctg ttgaaagcgc
     1741 tcaggaaaat gctgacgatg ataatcactt taccagcacg cagaacgaag cccttcagac
     1801 gctgccaggt ctggataatc aggcttttaa cgtgtggtac atggtagacc ggcagctcca
     1861 tgacaaacgg cgtcgcttca ccgcgcatga tggtgtactt gagcatcagg ccagtcagca
     1921 ccgccatcac gataccgagc atatacagcg agaaaaccgc cagggcaccg ttctgcccga
     1981 agaaggcagc cgcgaatact gcgaagatag ccagacgcgc gccgcaggac ataaacggtg
     2041 ccatcatgat ggtcatcaga cgttcacgcg gtgcatcaag cgtacgtgcg cccattaccg
     2101 acggtacgtt acaaccgaaa ccgacgatca gcggcacaaa ggatttcccc ggcaagccca
     2161 gcgcctgcat caggcggtcc atcacaaacg ctgcacgcgc catatagccg gagtcctcaa
     2221 ggaaggagag gaacaggtac atcatgccga tctgcggcac cagtggcagc acggtgttaa
     2281 taccgccacc caggccctgg gcgaggaaga tagtcagcca gtccgggaag tggagcgtgt
     2341 agccaatcca ttgaatacca tgcacaaata gcgccacgga gccgacgtca aacagcggct
     2401 gtaacgcccc gccgatgttg atagccagca ggaacatcag gtacatcaca aagaggaaaa
     2461 tcggcagacc gaggaaacgg ttgagcacga ttttatctac cgcagtggtg aaacggctgg
     2521 gttctgccgt cagggtgttg cttaccacat cacagatggc agctatgcac tggtaacgcg
     2581 catcggcaat gtgcagcgcc ggatcatcca tttcattacg cagacgggca agggcggcat
     2641 ccagatgctg cgacgcttca ccggcgtagg cgcggctgta gatatcgcct tccagcattt
     2701 gcaggcccag ccagcgacgt tgtttcagcg ggatgtcgga aggcatcact tttgccagtg
     2761 aatctgcttc gttgagtagc ggctgtgcgt aatgcaccag ttccacattc tcgttagctt
     2821 tatagcgatc aatcgccagc ttgagcgctt caataccgcg accacgggtt gaaaccagcg
     2881 ggatcactgg gcagccaaga cgcgccgaca gagcatcaat ttcaatacga atattttgct
     2941 tctcggcaat gtcgagcatg ttcagtgcca cgatgcaggg aatgccgagt tccagcagtt
     3001 gtagcgtcag gtacaggtta cgctcaaggt tagacgcatc caccacgtta atcagcaggt
     3061 cggcgtcgcc actcaaaatg tagtgacagg cgatttgctc atcgagcgag gtctgcgatg
     3121 agatggtggt cagagaatag gtgccgggca ggtccaccag cgtgacctga tgatcggtgg
     3181 tggagaattg cccttcttta cgttcgacgg taacgccagc ccagttacct acacgctgac
     3241 gtgcgctagt gagctggtta aataaggttg tcttgccaga atttggatta ccaattaagc
     3301 caatggttaa ttttttcatt gttgttatca ccgtattaac aggaaaccgc ttccacttct
     3361 aataaggcca gatctttttt gcgtaatacc aggctcacac gacgggtttc gatatgaatg
     3421 gggtcgccga gtggagcgac gcgcaccaca ttaaaagagg agccaggtaa catgccaaga
     3481 gaaagcagtt tttggcgata tgccgggctg atttcacggg aaaagccagt gattttccac
     3541 gcagtatctg gagtgtattg cataggtgcc tacttgtttc tcattaactg gataactacc
     3601 tcatggggcg tttgacagcg acatgagacc agatgatttg ccaacaaagg agagattaaa
     3661 taatcaacaa cacaatgata atgagaatgg tttctatcag caatacttaa aatgtgtgcg
     3721 aatgttggtt tgaataataa tctgactcaa tgttgatgtg gctcaaggtt atatcgcagc
     3781 gaaaatataa ttcgaatagt gaattacccg gactggctaa tgtttaatta aggtttcagt
     3841 tgttaatgaa gtgataaata ttgccggaat atattatgag tttggttgtg gaataaaaag
     3901 gccggagata ttctccggca cttttatgtc aggcaaggcg ttcgttgcca gatgcggcgt
     3961 gaacgcctta tcgcgcaggt ttaggggcca gtccgggcgt ttaacgtttt ttgcccattg
     4021 ccgccgccag cgcatccatc atcgcgctat taccggcagg ctgcgcttca cgaccgcgtg
     4081 gtttggctgc cgggcggttg ttttgcgggc gttcattacc gccgccgcga cgggcgttgg
     4141 tttcgccagg ctgctcgtcc agacgcatgg tcagggcgat acgtttacgc tgaagatcca
     4201 cttccagcac tttcaccttc acaatgtcgc ccgctttcac cacggtatgc ggatcttcca
     4261 caaacttgtt cgacaatgaa gagatgtgaa ccaggccgtc ctgatgcacg ccaatatcga
     4321 caaacgcgcc aaagttggtg acgttggtga cagcgccttc gaggatcata cccggttgca
     4381 ggtcgttcat tgtctcgacg ccatcggcaa actgagcggt tttaaattcc ggacgcggat
     4441 cgcgacccgg tttttccagc tctttgatga tgtcagttac tgtcggcaca ccgaattttt
     4501 catcagtaaa gtcagacgct ttcaggttac gcagttcgct gctgttaccc atcagatctt
     4561 tcagtgcctg ctgtgttgct gccagaatgc gttccaccac cggataggct tccgggtgaa
     4621 cggtagaggc gtccagcggg ttatcaccgt ggttaatgcg caggaagccc gcgcactgct
     4681 cgaaggcttt cggccccaga cggctgactt tcagcagttg ctgacggttc ttgaactggc
     4741 cgttctcatc gcgccaggcc acgatgtttt gcgccatcat gcgcgtcagg cccgccacgc
     4801 gagttaatag cggaacagaa gcggtgttga gatcgacgcc aacggcgttt acgcagtctt
     4861 ctactactgc atccagtttg cgggccagtt gcgtctggct gacgtcatgc tgatactgac
     4921 ctacgccgat agatttcgga tcgattttca ccagctccgc cagcggatcc tgcaaacggc
     4981 gggcgataga taccgcacca cgcagcgaaa cgtcgagatc cgggaactcc tgcgctgcca
     5041 gctcggaagc tgagtaaacc gacgcgccag cttcgctgac gatcactttc tgtgcggtca
     5101 ctttcgggaa ctgcttctgc acgtcgagat agaaacgttc agtttcgcgg gaagctgtac
     5161 cgttgccgat cgccaccagt tcaacgttat gtttttcaca caaggcagca acggtcatcg
     5221 ctacttttgc ggcttgtccg gtgtgcgggt aaatggtatc ggtcgccacc agtttgccag
     5281 tggcatcgac cactgccact tttaccccgg tacgcagacc cggatcgagg cccatcgttg
     5341 cacgcagtcc ggcaggggcc gccatcagca gatcgtgcag gttacgggca aagacgttga
     5401 ttgcttcatc ttccgcgcgt tcgcgcacgg tacccatcag ttcggtttcc agatgcatca
     5461 gcaccttgat gcgccaggtc cagctcacta cgcctttgcg ccagctatcc gccggggcat
     5521 tgttcaggcg caggccaagg tgatccatga tgatttgctc gcaatagctc tctttgggcg
     5581 gctcatcgaa ctgcggatcg gcattcagcg aaagctggag tacgccttcg ttacgcccac
     5641 ggaacatcgc cagcgcgcgg tgagaaggca ccgtggacaa cggttcgtga tgatcgaaat
     5701 agtcgcggaa tttcgcccct tcctcttctt taccgctcac caccgtagaa accaaatgcg
     5761 cgttcttcca cagataatca cgcactttcg ccagcagcgc ggcatcttcg gcaaaccgtt
     5821 ccatcaggat atagcgcgcg ccgtccagcg cggctttggt atctgccacg cctttatcgg
     5881 catcaatata ttgcgcagcg gcgacttctg gtgtgtgtga cggatcgctc cacagcaggt
     5941 cagccaacgg ctcaagccct gcttcaatgg cgatttgccc gcgggtgcgg cgtttaggtt
     6001 tgtagggcag gtagaggtct tcgagttcgg ttttgcttag ggtggcgttg atggccttcg
     6061 ccagatcatc ggtgagtttg ccttgctcgg aaatggactt gaggatcgcc tgacgtctct
     6121 cttccagctc gcgcagatag ctcagacgcg tttccagatt acgcagctgc gtgtcatcca
     6181 gaccgccggt gatttcctta cgataacgtg cgataaacgg cacggtattc ccttcgtcaa
     6241 gcaggcgaac ggcagcgtca acctgttccg ggcgcgcctg aatttcaccc gcaataatgc
     6301 ggcagaacga atcattcatc atggtttgag ttcatctttt cggatcaaaa atcaggggat
     6361 agttatacgg actggctggc aaaaatgcca gccatcggca ggaggttaag actcttcatt
     6421 acggtttcac gtattcgata gcattaacat accagctcgc ttccccggca ggggtattca
     6481 ccaccgccag atcgccgact tcttttttca gcaatgcgcg ggccatcggg gaatcgatag
     6541 agatgtaatc tttacggcca aaaatttcat cgtagccgac aatacggaaa cggtgagtca
     6601 cgccatcgtc gttttcaatc tccacccacg cgccaaaaaa gactttgcct tcctgctgag
     6661 gggagtaatt gacgattttg agattttcca tgcatttagt gagatagcgc acgcgacggt
     6721 cgatttcacg cagacgcttt ttattatact gatagtcagc attttcgctg cggtcgccca
     6781 gacttgcggc ccaggtcacc ttttttgtga cctccgggcg ttcttcacgc cagagataat
     6841 taagctcttg tttgagtttt tcataccctt cccgggtaac caggggcgtt ttcatctcgt
     6901 tgattccctt tgtctgtttg ataatgcgca cattgggtat aacgtgatca tatcaacaga
     6961 atcaataatg tttcgccgaa taaattgtat acttaagctg ctgtttaata tgctttgtaa
     7021 caatttaggc tgaaattcat accagattta gctggtgacg aacgtgagct tttttaagaa
     7081 tacacgctta caaattgttg cgaacctttg ggagtacaaa caatgcaaga gaactacaag
     7141 attctggtgg tcgatgacga catgcgcctg cgtgcgctgc tggaacgtta tctcaccgaa
     7201 caaggcttcc aggttcgaag cgtcgctaat gcagaacaga tggatcgcct gctgactcgt
     7261 gaatctttcc atcttatggt actggattta atgttacctg gtgaagatgg cttgtcgatt
     7321 tgccgacgtc ttcgtagtca gagcaacccg atgccgatca ttatggtgac ggcgaaaggg
     7381 gaagaagtgg accgtatcgt aggcctggag attggcgctg acgactacat tccaaaaccg
     7441 tttaacccgc gtgaactgct ggcccgtatt cgtgcggtgc tgcgtcgtca ggcgaacgaa
     7501 ctgccaggcg caccgtcaca ggaagaggcg gtaattgctt tcggtaagtt caaacttaac
     7561 ctcggtacgc gcgaaatgtt ccgcgaagat gagccgatgc cgctcaccag cggtgagttt
     7621 gcggtactga aggcactggt cagccatccg cgtgagccgc tctcccgcga taagctgatg
     7681 aaccttgccc gtggtcgtga atattccgca atggaacgct ccatcgacgt gcagatttcg
     7741 cgtctgcgcc gcatggtaga agaagatcca gcgcatccgc gttacattca gaccgtttgg
     7801 ggcctgggct acgtctttgt accggacggc tctaaagcat gaggcgattg cgcttctcgc
     7861 cacgaagttc atttgcccgt acgttattgc tcatcgtcac cttgctgttc gccagcctgg
     7921 tgacgactta tctggtggtg ctgaacttcg cgattttgcc gagcctccag cagtttaata
     7981 aagtcctcgc gtacgaagtg cgtatgttga tgaccgacaa actgcaactg gaggacggca
     8041 cgcagttggt tgtgcctccc gctttccgtc gggagatcta ccgtgagctg gggatctctc
     8101 tctactccaa cgaggctgcc gaagaggcag gtctgcgttg ggcgcaacac tatgaattct
     8161 taagccatca gatggcacag caactgggcg gcccgacgga agtgcgcgtt gaggtcaaca
     8221 aaagttcgcc tgtcgtctgg ctgaaaacct ggctgtcgcc caatatctgg gtacgcgtgc
     8281 cgctgaccga aattcatcag ggcgatttct ctccgctgtt ccgctatacg ctggcgatta
     8341 tgctattggc gataggcggg gcgtggctgt ttattcgtat ccagaaccga ccgttggtcg
     8401 atctcgaaca tgctgctttg caggtcggta aagggattat tccgccgccg ctgcgtgagt
     8461 atggcgcgtc cgaggtgcgt tccgttaccc gcgcctttaa ccatatggcg gctggtgtta
     8521 agcaactggc ggatgaccgc acgctgctga tggcgggggt aagtcacgac ttgcgcacgc
     8581 cgctgacgcg tattcgcctg gcgactgaga tgatgagcga gcaggatggc tatctggcag
     8641 aatcgatcaa taaagatatc gaagagtgca acgccatcat tgagcagttt atcgactacc
     8701 tgcgcaccgg gcaggagatg ccgatggaaa tggcggatct taatgcagtg ctcggtgagg
     8761 tgattgctgc cgaaagtggc tatgagcggg aaattgaaac cgcgctttac cccggcagca
     8821 ttgaagtgaa aatgcacccg ctgtcgatca aacgcgcggt ggcgaatatg gtggtcaacg
     8881 ccgctcgtta tggcaatggc tggatcaaag tcagcagcgg aacggagccg aatcgcgcct
     8941 ggttccaggt ggaagatgac ggtccgggaa ttgcgccgga acaacgtaag cacctgttcc
     9001 agccgtttgt ccgcggcgac agtgcgcgca ccattagcgg cacgggatta gggctggcaa
     9061 ttgtgcagcg tatcgtggat aaccataacg ggatgctgga gcttggcacc agcgagcggg
     9121 gcgggctttc cattcgcgcc tggctgccag tgccggtaac gcgggcgcag ggcacgacaa
     9181 aagaagggta aataaacggg aggcgaaggt gcctcccgtt ttgctttcta taagatactg
     9241 gatagatatt ctccagcttc aaatcattac agtttcggac cagccgctac cagcgcggca
     9301 cccgcagggg tgtcggtgta tttatcgaag ttgtcgataa acagtttcgc cagggtttcg
     9361 gctttttcct gccactgttc cggagaagcg taggtgttac gcggatcgag aatcttcgtg
     9421 tctacgcccg gcagttcggt tgggatcgcc aggttaaaca tcggcagagt gaaggtttct
     9481 gcattatcca gcgaaccgtt gaggatggcg tcgataatgg cgcgggtatc tttaatcgag
     9541 atacgtttgc cagtgccgtt ccagccagtg ttaaccagat aagcctgcgc gcccgccgcc
     9601 tgcatacgtt tcaccagcac ttctgcgtac tgagtcgggt gcagcgacag gaacgccgcg
     9661 ccgaagcaag cggagaaggt tggcgtcggt tcggtgatgc cacgctcagt accggccagt
     9721 ttggcggtga agccagagag gaagtgatac tgggtttgat cggcagtcag gcgagaaacc
     9781 ggcggcaaca cgccgaaagc atcagcagtc aggaagataa ccttagtcgc gtggcccgct
     9841 ttggaaaccg gcttaacgat gttatcgatg tgatagattg gataagaaac gcgggtgttc
     9901 tcggtttttg aaccatcatc aaagtcgata gtgccatctt cacgcacggt gacgttttcc
     9961 agcagcgcat cacgacggat agcgttgtag atttcaggtt ccgcttcttt cgacagcttg
    10021 atagtttttg cgtagcagcc gccttcgaaa ttaaacacgc cgtcatcgtc ccagccgtgt
    10081 tcgtcatcgc caatcaggcg gcgtttcggg tcggtggaaa gggtggtttt accggtgccg
    10141 gaaaggccga agaacaccgc aacatcgcct ttctcgccaa cgttggcgga gcagtgcata
    10201 gaagcgatac ctttcagcgg cagcaggtag ttcatcatcg agaacatccc tttcttcatt
    10261 tcgccgccgt accaggtgcc gccaatcagc tgcatgcgct cggtcaggtt aaacgccacg
    10321 aagttttcgg agttcagacc ctgttctttc cactgcgggt tagtgcactt cgcgccgttc
    10381 ataacgataa agtctggttt gaaacctgcc agctcttcat cgctcgggcg aataaacatg
    10441 tttttgacaa aatgcgcctg ccaggccact tcggtgatga aacggacgga aagacgagta
    10501 tccgggttcg caccacagaa agcgtcgaca acgaacagac gtttgccgga aagctgcctg
    10561 gtcaccaggc ctttcagatg ctgccaggtt tccggagaga gaggtttgtt gtcgttctta
    10621 cctttgcctt tgtctgccca ccagaaagta tcgcgagtgg tatcgtcacg gacgatatac
    10681 ttatcttttg gtgaacgacc ggtgaagatc ccggtatcga cggcaacggc acccagatta
    10741 gttaacaccc cgcgctcata acctgtcagg ctcggatcga gctcttcctg atacagcagg
    10801 tcgtagcttg ggttgtaaac gatatcatgt acgtcactga taccataagc ctcgagttct
    10861 tgcggggtca aaccattgtt aacgcgcatt tcactgctcc ttagccaata tgtattgcct
    10921 gaatagtaaa gtctttttgg gggtgttaac cgcgacaagg ctcatagatt tacgtatctg
    10981 gagaaattca tatttgacaa aaaactccgt gattcctgtc acgaaacggt tgctattatc
    11041 gcagaaatag cattttaggt gtggaaaatg ttctcaaaag gttaaatctt ggtttatatg
    11101 gcgggaatat tgtgagtgga atcgcattcc tgaatgaaat tgatgcaaat tcaaccggta
    11161 aagtatcgat tcaccacgct aacttttttt gattattttc tgaaaaaagc aaaatactac
    11221 tgcccggatg cgataattaa cccacctttt gattctggat aaccaacatg gataacgttg
    11281 aactttcacc ggcaacacgc tgggggatga ttgctaccgg attacttcag gggctggttt
    11341 gctacctgct tatcgcctgg cttgccggaa aaaatcacag ttggattgtt tatggcgtgc
    11401 ctgcgaccgt cgcgttttca tccgttttac tcttctccgt gatctctttt aaacagaagc
    11461 gcctttgggg atggctggcg ctggtgttta ttgccacgac agggatgagc ggttggctga
    11521 agtggcaaac tgatggcatg aacccctgga gagctgaaaa ggcgatttgg gattttggct
    11581 gatatctgct gttgatggca atgttgttgc taccgtggat acaacaaagc cttcgtatcc
    11641 gcaatggcag tagccgctat agctactttt accagtcagt atggcataac gtacttatat
    11701 tactggtgat ttttctctcc aatggcctga cgtggctggt gcttttactg tggggtgagt
    11761 tgtttaaact tgttggcatc acgtttttca atacgctctt ttttgcaacc gactggttta
    11821 tgtatcttac atttggtctg gttaccgcac tggtggtgat cctcgcgcgt acacagtcac
    11881 gtttaatcga ctctattcaa aagttgttca cgctaatcgc cacggggttg ctgccgttag
    11941 tatcattgct aaccctgatg tttatcatca ccctgccgtt tacggacctg agcgcgattt
    12001 ctcgccacat ctccgccgcc gggttgctgt taacgctggc ctttttgcaa ttgatcttaa
    12061 tggctattgt ccgcgatccg caaaaagcgt cacttccctg gacggggccg ttgcgttgcc
    12121 tgattaaaac cgctttgctg gttgctccgc tgtatgtgtt cgtcgccgcc tgggcgttat
    12181 ggctgcgggt cgctcagtac ggctggactg tcgaccgctt gcagggcgcg ctggcggtgc
    12241 tggtgttact ggtgtggtcg ctggggtatt ttgtcagtat cgtctggcgt aatgggcaaa
    12301 atccccttgt tcttcagggc aaagtgaacc ttgcggtttc gttattggtg ttggtgatac
    12361 tggtgcttct taattcgccg gtgctggaca gtatgcgcat tagcgtgaac agtcatatgg
    12421 cgcgttatca gagcggcaaa aacacgccag accaggtaac tatctacatg ctcgaacaga
    12481 gcggtcgcta tggacgtgcg gcgcttgagt cgctgaaaag cgatgccggg tttatgaaag
    12541 acccgaaacg cgcgcgggat ctgctgatgg cgttagatgg agagcaacat cttcaggaac
    12601 aggtatcgga aaaagtatta gccgaaaatg tgttaattgc tcctggttct gttaaaccgg
    12661 atgcgacatt ctggtcggcc ttaatccagg atcgctataa cgtgatgacc tgtattgaaa
    12721 aagacgcctg cgtcctggtc gagcaagatc tgaatagtga tggtcaggcg gagcggatcc
    12781 tgtttgcttt taatgatgac agagtcattg tctatggctt tgactcagac agaaaagaat
    12841 gggacgcgct tgatatgagt ttacttccga acgaaataac gaaagaaaaa ttactcacag
    12901 ctgcgaagga tggaaaactg gatctgacgg tagatggtga aacgctggaa ataaacttaa
    12961 gtaagtgaca actcttttaa cggatacgac gctgtatctg accaacaata atttgcgatt
    13021 tatttcacag gcctggtaag cgcagcacat caggccatta cgtcgtaaaa tccggcagag
    13081 ccctctctgc cggacatact cattaatgaa cttgcggatc tgccggagac gcgttgttgc
    13141 ggatttcagc aatatccatc gcattgaaca gatagtggtt accgcagtaa tcacaatgca
    13201 tgtcaatttc gccatcttcc gccaggatgc tatcgacttc ttcatcaggc agcgttttca
    13261 gcgcgtcggc gcaacgttca cgcgagcagg tgcatttgaa ctccacatcc tgcggatcgt
    13321 aaaccgtcac ctcttcttcg tgatacaaac gccacaacac ttcgtttgcc ggtaaggtca
    13381 gcagttcttc ggttttgatg gtttcggtta gcgtcgccag gtggtcaaag tcgtcctgct
    13441 gggcattttg cgcaggcatt acctgcaaca acataccgcc tgcagccggt ttgccgtcta
    13501 cgtcgccggt gcgaataaac aggcgcgtcg gcagctgttc agaacgcata aagtaatctt
    13561 ccaggcaggc cgccagggta tcaccttcca gaccaaccac gccctgatag cgttcgcctt
    13621 cgctcggggt aatggtgatc accacgtaac cattgccgac cagcgttttc aggtcggcat
    13681 tttctggaat ttcgccctgc acgcgcgcca caccgcgcat ttgctggtta ttgttaccgt
    13741 taataaccgc cagattcatc ggaccgtcgc cttgcagctg tacggtgata tcaccatcaa
    13801 acttcagcgt agcggttaac aggctggtcg caaccagcag ttctgccagc acgtttttaa
    13861 cgggctgcgg ataatcgtga ttctcaagga tctgttgcag ggtttccgaa acggttacca
    13921 gttcgccgcg cacggcaaag ttttcaaaca gatagcgatg taattggtca tgttgcggca
    13981 taatcatctc tcttgcaggt gacagttatt cactgtcgcc gtgtttaaat cgtaacaggt
    14041 cgcggcgctc ttttttgtcc gggcgtcggt ccgggtgcgg catggttaag gcattaagtt
    14101 tacgtgccag cgccattttt tcgcgtttct ctacactttc cgcagtctct tcatacagca
    14161 aggctgcctc gctggcgggg cgacgctgtt cagtaatcgc ctttacaatc accgtgcgtt
    14221 cgtcatttcc ctggcgcaga gtgagcgtgg cattcagctc gacgattttg ctcggcttgc
    14281 tgcgctgccc gttgtaatgc accttaccgc cttcaatcat ttcacgggcc agcgcgcggg
    14341 ttttataaaa acgggcagcc catagccatt tatccagtcg aacctcaaca gcaggttttt
    14401 ctttcatggc gtctccttca cattagcgag gggatcaggc ggcggtagtc attcagtgac
    14461 ggatggcgtt gatactgttt ctcggcaatc ccggaatcag gattagtcac gccgaggcag
    14521 taacgaatac caaattgcgc ggcagcatcg agaatcgctt cgctgtcatc aataaacagc
    14581 gttctttcag ctttcagacc cgtagcttcg gccaccgcat gccataaccg ctgatcctct
    14641 ttcggataac caaatgtgtg ggtggaaagt aataaatcaa ggtgtgcgtc cagaccggta
    14701 tgctcaagtt ttaccgccag gttgtgcgga tgcgcattgg tgagcaaaat tcgctgctta
    14761 ccgctggctt tcagtgcctc aagaaacgga atggtatctt cacgcagtac ggcacgcggt
    14821 cccatctcgg tggtcatcgc acagatatcc agacccagtt gctcactcca gtaatcaaga
    14881 cagtaccagt ttagcgtatg ctgtacgtcg tgatattgct ggcgcatata ttccatcgct
    14941 tcctgtggcg taaccccgtt tttcgcgccc catgtttcag gcaccagctt ttgccagaaa
    15001 tagttatcga aggcgaggtc gagcaacgtg ccgtccatat ccagcagaac ggtatctacg
    15061 tcctgccagg caatgttgat atgcatgagg gaaatctcca gagtgaagca atttgcgcga
    15121 cagggtagca taacctgccg cgcaaacgtg ttattcgata aggctttctg aaggggtgat
    15181 cagttgcggg ttcaggcagc tttcataata ggcctgaatc ttcatcatgc gagtgcgatg
    15241 acgctggtaa cgacgccagg cctgtacgcc attgtaaatg gcgctaccca gcatcgtcag
    15301 tagcagcaat gtggtgctga gatagcgcca caggccggaa cgatccggga ttggatgaag
    15361 gccaatatgc tgcgtaccat tggcgtcagt gaagattttt gtgacgatac cctcggcgtt
    15421 aaacggcgta tgcatcagca tttgtgccag tttctggaaa gcgttccact gttcttgcgg
    15481 cgggtagtcg taaagtgatg ccgaaggcca gggctgatca acaaaatcgc tgccttcgtc
    15541 gctgacaatc aggaatccgc cgggggccgg actgtttagt gattgtgccg ctcgcgccgt
    15601 ttcatgcgtg ataaacggcg cggtggaggt tgccaccagg ttatccagcg attccgcact
    15661 caccgggcgt aataacacat tcacgccatc gagcttcccg gcgttggcgc gttttaccag
    15721 cgcgtcccag tctttactgt tgccgagatt gaccagcgca tttttcagtc gcacacagtc
    15781 atctttggca gaacataaat ccgctgtctt cagtacaatg tcgccaaaat catcaagcaa
    15841 taccatgccg gatttttgaa ttgctgaacg taatgaggca ctgacgcgag atccatcttc
    15901 cggtttaggg tgcagctggc gattaactgc ttcagtcaat gccgtcgctt tgttgaccag
    15961 ttcagattct ggtaatggca atgagcgggc gtcgttccag atgatctgcg agcagtcaaa
    16021 cggtaaaaaa ggtgaattgg ttttcgcgct ccaggttccg gaagtgcgaa tattacacat
    16081 tcccgtaccg ctgatacgca atgtatcgcc tacccgcacg ccagcgtcag ccagttgttt
    16141 tacgctggtg gcttcaatgg tctgtgcgcc tttcatccat gagagagtga atttcagcgg
    16201 catatccagc gggatccaga ataacagcat aaacagcacc agcagcgagc ccgcagcgat
    16261 aatcgtactg cgcagccagt gctgtaacgg aaagtttttt acttcatcat gcagcgaaag
    16321 atatcgcccc tgacgcacta cgtggcggtc gagatagata tcaatatcgg tttgttgacc
    16381 gagatcctga gcaatgtatg gctgccagtg tgcgggatag accaggtcga taataccgag
    16441 tgaaatattg ttgatctgtt cctgatcgtt ttcgccaaac aatccccaac gacggggtgt
    16501 accgcgcaga caatgaattt cccgcaggga ggattttgcc gggggcgcga atagccccca
    16561 cagacctgcg cccagcagca gtaatgcgcc gcctgccagc cacggaacaa atacatccgg
    16621 ggtaatcagg cagaaaaaga acatcaggaa ggaggcgacg atcagcaacg cttcacgcag
    16681 cccgcgcgga cgactcagcg catattcttc atgcgtttct ttgcgaatat tgagtagctc
    16741 gatttgctca ctctcttccc cgcgaatcga cgcctgcgtg gacggaacag gttgcaaggc
    16801 atagccgcgc gtttcctgca tgtactcctg cagcgtatga ccgttgagtg aaataaccag
    16861 cggcagcgaa tcggtataaa tcagttcaac ggtattctca tcgttgatgt attgttccca
    16921 gaaagggggc aggtggactt ctaccgaatc gaggtagtaa cgccatttat tcggatcgtc
    16981 ggtagagatg ccgtaacgcg tgatagcgtg tgtcagcatc atgacgttgt tgctttcggc
    17041 attcagcgcc agagagatcg gtgccgcgct agctcccgtt gggccaggca cctgtaatac
    17101 ctgcgtcagg ctctcaagat aattttcaac ggcgctacgt tcttcaggtg tgagtttacg
    17161 cgtttgcgca tccgcgaagg cgttggtcca gggcagctga cgccgtctgg atcgcacttt
    17221 tatcagccat cccgcaagta gtgagcaggc cagcaaagca gctaaaaaaa tcacaatggt
    17281 gctcatgctt tccccatctt acttagtctg tcaggcgtgg tcagtcgcac cagacacaat
    17341 acatgattct gcggttggct atcggcaagc gtggagttta acttgagcat cattaagcac
    17401 atcacagtaa ccgctgatca tagcaaaccc tctgaaggcg ggatatcagc aaattcctgg
    17461 agttatttca acctattgac ttttaatttg atttgaggac gattatttat taagttacat
    17521 aaatgtaaat aacatgcaat tcacattgcc gaaagcgtgt ccgatatcgc acaataaccg
    17581 ctgatttcac agcatagatc ccacgatgag caaatcatta caaaaaccca ccattctgaa
    17641 tgttgaaact gtagcccatt cccgactgtt taccgtcgag agcgtggatc tggagttcag
    17701 caatggcgtg cggcgtgttt atgaacgaat gcgtccaacc aaccgggaag cagtgatgat
    17761 tgtgccgatt gtggacgatc acctgatcct gatccgcgaa tacgcagtgg gaactgaatc
    17821 ctacgaatta ggtttttcga aaggattaat tgatccgggt gaaagcgtct acgaagccgc
    17881 taaccgcgag ctaaaagaag aggttggatt tggtgcgaac gatctgactt ttttgaagaa
    17941 gctcagcatg gcaccgtctt acttttccag caaaatgaat atcgtggtcg cgcaagatct
    18001 ctacccggaa tcgctggaag gcgatgagcc agagccgcta ccacaggtgc gctggccgct
    18061 ggcgcatatg atggatttgc tggaagaccc tgacttcaac gaagcgcgta atgtcagcgc
    18121 gctgttcctc gtgcgcgaat ggttgaaagg gcaggggcga gtgtaaatgc gtgaagtttg
    18181 cctgatgcac aacgattatc aggcttatgg ggttcctgca atatattgat ttttcacgat
    18241 tttgttagcc ggataacgcg ttcacccgca tccggcataa acaaatgcac tttgtcagcc
    18301 aactgaaaag gcgccgaagc gcctttttaa tcagaacaat tcctgtgcct caccattatc
    18361 gataatggtt gttcccacct cgtgcaccgc ctgttgtgtc ggctgcgtac cttcgatgaa
    18421 atactcttcg cggctgttgc caccattagc taactgcccg gtgctgcgat cgatattcac
    18481 cgtcacaata cccggaggcg gcgtcagcgg ctgctccggc acaccttcaa gaacggcttt
    18541 catataagcg tcccatgcag gctgggcact cttggcaccg ccttcgtaac ctgagatctg
    18601 atctttaatc gctccggaag ccgttgtatg accgagatta cgacggtgat catcaaagcc
    18661 aatccagacc gaggtcacaa cgcccggacc gtaacccgag aaccacgcat ctttcgaact
    18721 gttagtggta ccggttttcc cgccgatatc gcgacgctgc aaatcacgac ctgcacgcca
    18781 gccggtaccc tgccagcctg gttcaccaaa gatattggtg ttcaaagcac tcttaatcag
    18841 gaatgccagc ggagtgttga tgacgtgcgg tgcgtactcc tgcgcgccag tcttcgccac
    18901 taacgcctga tttgcctgct ccagctgcgg cattggtaca gaaacattct gctgctcgcg
    18961 ggagatagcg acatcttcaa catcgttatt ttccagcacg ttcgatttct gcgtatcacc
    19021 gtaaatcacc ggaatatcgc attccgggca ggctactttc ggtttcgctt cgaaaatcac
    19081 gccgccctga tcgttttcaa ttttgctgat aaaccacggg tccaccagga agccgccgtt
    19141 cgccatgacc gcgtagccgc gcgccacctg cattggggtg aaggacgctg aacccagcgc
    19201 cagcgattcg gtgtggacaa tgttttgcgc cgggaagccg aagcgttgca gatattctgc
    19261 agcgtagtcg acgcccatcg cccgcattgc acgtaccatc accacgtttt tcgactgacc
    19321 cagcccctga cgtaagcgaa ttggaccagc atactgcggt ggtgagttct tcggctgcca
    19381 gtcagaaccg acacctgcat cccagcgaga aattggcaca tcgttcaaca tacttgccag
    19441 cgtcagacct ttatccatcg ccgcggtgta gaggaacggt ttgatgttgg aacccacctg
    19501 acgcagtgcc tgggtggcgc ggttaaactt gctctgattg aaatcaaagc caccgaccag
    19561 cgccataacg gcaccatttt gcggattgat cgacaccagc gccgagttca cttccggcac
    19621 ttgtgccagc caccatgcat cgccaacctg acgaacccag atttgctgac ccgtttgcag
    19681 aacatcggtc actttacgcg gcgtcggtcc ctgctgagta tccgaacggt aaggacgcgc
    19741 ccagcgaacg ccgtccatac tcaatgcgac agtcgacccg tccgccagca ttgccgtcgc
    19801 ttcctgagga ttggcgctgg tgactgcggc aggcagcagc ggaccatagg tcggcagcgc
    19861 cttcagcgta tcggtaatct tgttgttatc ccacgccgac tcgcccactt tccacagcac
    19921 atttgccggg ccgcgatagc cgtggcgcat gtcgtagtcc agcacgttat tacgtaccgc
    19981 ctgctgtgcg gcctgctgca ctttgcgggt gatggtggtg taaatgcgat aaccgtcttc
    20041 ataggcactt tcgccataac ggttatacat ctcctggcgc accatttcgc tcaggtacgg
    20101 cgcagagaaa gcaatctccg gcgcgtgata gttagcgtta atcgcctcag tgcgtgtctg
    20161 atcgaactgt tgttgggtga tatacccttc atccagcatc cgcgacagta cgacgttacg
    20221 ccgcgcgacg gcacgatcca ttgagtagag cggattaaag gtggaaggcg ctttcggcag
    20281 cccggctatc acagccattt cgttcagcgt cagttggtcg accgttttac cgaaatagac
    20341 ttgtgccgca gcaccgacac cataggcgcg gtaaccgagg taaatcttgt tcagataaag
    20401 ctcgaggatc tcgtctttcg tcagcaactg ttcaatgcga atcgcgagga agacttcctt
    20461 aatcttacgc atcagcgtgc gttctggact gaggaagaag tttctcgcca gctgctgggt
    20521 aatggtactc gccccttgtg acgcgtggcc ggagaacagc gccacacttg ccgcacggaa
    20581 gatccccacc gggtcaacgc cgtgatgctc gtagaagcgg ctgtcttccg tcgcgataaa
    20641 ggctttcacc atctccggcg ggatttgatc caacgtaacc ggaatacgac gtttctcacc
    20701 gtattgagca atcagctcgc catcggcgct gtaaatctgc atcggaattt gcaggcgaac
    20761 atcttttaat gtcgccacat ccggcagttg tggctcgatg tagcggtata ggccataaat
    20821 cgagcctgct cccagcagaa tgcaacagac tgcaaggatc aaaaaatact ttacgaactt
    20881 cactggaaat ttcccattta gtttcatttg ggcagtttat aaacaaacgt gcggtagtat
    20941 aaaggcaagc cagacgcatt gatatacccg tcagagtgac gggtgataag gagatcatca
    21001 caatggcatt taagatctgg caaattggtt tgcatttaca acagcaagaa gcagtagcgg
    21061 ttgcgatcgt gcggggcgca aaagaatgct ttttgcaacg ctggtggcgg ttgccgctgg
    21121 agaacgacat tatcaaagat gggcggattg ttgatgcgca gcggctggct aaaacgttgt
    21181 taccgtggag tcgcgaactg ccgcagcgtc atcacattat gttggcgttt cccgccagtc
    21241 gcacattaca gcggtcattt ccgcgcccgt cgatgtccct cggtgagcgg gagcaaacgg
    21301 cctggctgtc agggacgatg gcccgcgagc tggatatgga tccggactcc ctgcgtttcg
    21361 attacagcga agactcactc agtcccgcct ataacgtgac tgccgcgcaa agcaaagagc
    21421 tggcaacgct actcactctg gcagaaaggt tgcgtgttca tgtgagcgcg atcaccccgg
    21481 atgccagtgc attacagcga ttcctgcctt ttttaccttc tcatcagcaa tgtctggcct
    21541 ggcgtgataa cgaacagtgg ctgtgggcga cacgctgtcg ctgggggcgc aaactggcgg
    21601 tggggatgac tagcgcgaag gagctggcgg cagcgttatc cgttgatccc gaaagcgtcg
    21661 cgatatgtgg cgaaggcgga tttgatccct gggaggccgt ttctgttcgt cagccgccgc
    21721 taccgccgcc aggtggagac tttgccatcg cgctggggct ggcgcttggg aaggcgtact
    21781 gatgaacccg ccaattaatt ttttgccctg gcgacagcaa cgccggaccg cttttcttcg
    21841 tttctggttg ctgatgttcg ttgcgcctct gctgctggcc gtcgggataa cgctaatact
    21901 gcgtctgaca gacaacgccg aagcacgcgt aaacaccgtt ttgcttcagg cggaacaaca
    21961 actcgcccgc agcttacaga taacgaaacc acgtttgctg gagcgccaac aaattcgtga
    22021 gcagcgtttg caacggcagc gccagcgaca atttacccgc gactggcaat ctgcgctgga
    22081 agcactggcg gcgcttttac ccgagcacgc ctagctgaca acgataagct ggcagcaggg
    22141 aacgctggag atcaaggggc tgacaacaag cattaccgcg ttaaacgcac tagaaacgtc
    22201 actccgccag gatgcgtctt ttcatctcaa tcagcgggga gccacgcagc aggatgcgca
    22261 gggacgctgg caatttgagt atcagttaac aaggaaggtt agcgatgaac atgttctttg
    22321 actggtggtt cgccacatca ccccgcctcc gccagttttg ctgggcattc tggttgctga
    22381 tgttagttac gctcattttt ctgtcatcga cacaccatga agagcgcgac gcattaattc
    22441 gactacgggc aagtcatcac cagcagtggg ccacactgta tcgcctggtt gacaccactc
    22501 ccttcagcga agaaaaaacg ctgccctttt cgccactgga ttttcagttg cccggcgcgc
    22561 aactggtttc ctggcatcca tccgcgcagg gaggcgagtt ggcgttgaaa acgctgtggg
    22621 aagcagtgcc gtcggcattt acacggctgg cagagcgcaa cgtcagcgtg agccgttttt
    22681 cattaagcgt ggaaggtgat gatcttttgt tcacgctaca actggagacg ccgcatgagg
    22741 gttaaacgct ggttgttggc aggtattgta ttgtgccttt taaccggtat gcgtgaccct
    22801 tttaaaccgc cggaagatct atgccggatt agcgaactta gccagtggcg ctatcagggg
    22861 atggtagggc gaggcgagcg catcatcggt gtaataaaag acgggcaaaa gaaatggcga
    22921 cgggtgcagc aaaacgatgt gctggaaaac ggctggacaa ttttacagct gacgccagac
    22981 gtactaacgc tgggtaccgg gacaaactgc gaaccgccac aatggttgtg gcaacggcaa
    23041 ggagatacaa atgaagcaat ggatagccgc actactgttg atgctgatac ccggcgtaca
    23101 ggcggcaaag ccgcaaaaag tgacgctgat ggtggatgac gttccggtag ctcaggtgtt
    23161 gcaggcgctg gctgaacagg agaagttgaa cctggtcgtg tcgccagacg tcagcggtac
    23221 ggtgtcgtta catctaacag atgttccctg gaagcaggca ctacaaactg tagtgaaaag
    23281 cgccggactg ataacgcggc aggaaggcaa cattctctca gtgcattcca ttgcctggca
    23341 gaataacaat atcgcccgcc aggaggcgga gcaggcgcgg gcgcaggcaa atctgccgct
    23401 ggaaaatcgc agtataaccc tgcaatacgc cgacgcggga gaactggcga aagcggggga
    23461 gaagctactg agtgccaaag ggagtatgac cgtcgataaa cgcaccaatc gccttttgct
    23521 acgagataac aaaacggcgt taagcgcgct tgaacagtgg gtagcgcaaa tggatctgcc
    23581 ggtcgggcag gttgagctgt cggcgcatat tgtcaccatt aatgaaaaaa gtttgcgtga
    23641 gttaggcgtg aaatggacgc tggccgatgc gcaacacgct ggtggcgttg ggcaagtcac
    23701 cacgcttggt agcgacctct ccgtagcgac ggcgacaacg catgtcggtt ttaacattgg
    23761 gcgcatcaac ggacgcttgc tggatcttga gctttccgcg ctcgaacaaa aacagcagct
    23821 ggatattatc gccagtccgc gtctgctggc ctcacatctt cagcctgcca gcattaaaca
    23881 ggggagcgaa attccatatc aggtttccag cggggaaagt ggcgcgacgt cggtggaatt
    23941 taaagaggcc gtcctgggga tggaagttac gcccacagtg ttacaaaaag gtcgcatccg
    24001 gctgaaatta cacatcagcc agaacgttcc ggggcaggtg ctacagcagg ccgatggcga
    24061 agtgctggcg attgataagc aggagatcga aacgcaggtc gaggtcaaaa gcggagaaac
    24121 gttggcgctg ggcggcattt ttacccgtaa aaataaatcg ggtcaggata gcgtaccgtt
    24181 gcttggcgac attccctggt tcgggcaatt atttcgtcat gacggaaaag aagatgaacg
    24241 acgcgagtta gtggtgttta tcacgccacg actggtttcc agtgagtaaa cagccgtaaa
    24301 agcggtaatg tttttacgct gaacgtgttt catctatttg acgcgcgcag gtatttagca
    24361 tacaaggagt accgatttga gagttggtgc tcttcgctgc ctgcgttcca tgatgatgat
    24421 ttatcattca ggcggcattt tgctgtcttt tttacgctaa tcttacccgg tgatttatcg
    24481 ccagagcggt ggtagcaagg cagcgcgctt gcagcgacca gatatgcaga gggatgggtg
    24541 atttattcag ttgccaaacc cgctggagta ttgagataat tttcagtctg actctcgcaa
    24601 tatcttatga ggtttcagtt catgtcctgc ggcgctctct gagcgaagcg ggtttatcat
    24661 taacgaatag tcttagtagt accgaaaaaa tggcagagaa acgcaatatc tttctggttg
    24721 ggcctatggg tgccggaaaa agcactattg ggcgccagtt agctcaacaa ctcaatatgg
    24781 aattttacga ttccgatcaa gagattgaga aacgaaccgg agctgatgtg ggctgggttt
    24841 tcgatttaga aggcgaagaa ggcttccgcg atcgcgaaga aaaggtcatc aatgagttga
    24901 ccgagaaaca gggtattgtg ctggctactg gcggtggctc tgtgaaatcc cgtgaaacgc
    24961 gtaaccgtct ttccgctcgt ggcgttgtcg tttatcttga aacgaccatc gaaaagcaac
    25021 ttgcacgcac gcagcgtgat aaaaaacgcc cgttgctgca cgttgaaaca ccgccacgtg
    25081 aagttctgga agcgttggcc aatgaacgca atccgctgta tgaagagatt gccgacgtga
    25141 ccattcgtac tgatgatcaa agcgctaaag tggttgcaaa ccagattatt cacatgctgg
    25201 aaagcaacta attctggctt tatatacact cgtctgcggg tacagtaatt aaggtggatg
    25261 tcgcgttatg gagaggattg ttgttactct cggggaacgt agttacccaa ttaccatcgc
    25321 atctggtttg tttaatgaac cagcttcatt cttaccgctg aaatcgggcg agcaggtcat
    25381 gttggtcacc aacgaaaccc tggctcctct gtatctcgat aaggtccgcg gcgtacttga
    25441 acaggcgggt gttaatgtcg atagcgttat cctccctgac ggcgagcagt ataaaagcct
    25501 ggctgtactc gataccgtct ttacggcgtt gttacaaaag ccgcatggtc gcgatactac
    25561 gctggtggcg cttggcggcg gcgtagtggg cgatctgacc ggcttcgcgg cggcgagtta
    25621 tcagcgcggt gttcgtttca ttcaagtccc gacgacgtta ctgtcgcagg tcgattcctc
    25681 cgttggcggc aaaactgcgg tcaaccatcc cctcggtaaa aacatgattg gcgcgttcta
    25741 ccagcctgct tcagtggtgg tggatctcga ctgtctgaaa acgcttcccc cgcgtgagtt
    25801 agcgtcgggg ctggcagaag tcatcaaata cggcattatt cttgacggtg cgttttttaa
    25861 ctggctggaa gagaatctgg atgcgttgtt gcgtctggac ggtccggcaa tggcgtactg
    25921 tattcgccgt tgttgtgaac tgaaggcaga agttgtcgcc gccgacgagc gcgaaaccgg
    25981 gttacgtgct ttactgaatc tgggacacac ctttggtcat gccattgaag ctgaaatggg
    26041 gtatggcaat tggttacatg gtgaagcggt cgctgcgggt atggtgatgg cggcgcggac
    26101 gtcggaacgt ctcgggcagt ttagttctgc cgaaacgcag cgtattataa ccctgctcaa
    26161 gcgggctggg ttaccggtca atgggccgcg cgaaatgtcc gcgcaggcgt atttaccgca
    26221 tatgctgcgt gacaagaaag tccttgcggg agagatacgc ttaattcttc cgttggcaat
    26281 tggtaagagt gaagttcgca gcggcgtttc gcacgagctt gttcttaacg ccattgccga
    26341 ttgtcaatca gcgtaacaac aagaaaggtc aggccgctta tcaagcggtc tattagcttc
    26401 aggttaattg caacgtggta agcattaacc ttttagtggg gtgttaaatg gatgaattca
    26461 aaccagaaga cgagctgaaa cccgatccca gcgatcgtcg tactggtcgt tctcgtcaat
    26521 cttctgaacg ttctgagcgt actgaacgtg gcgaaccgca gatcaatttt gatgatattg
    26581 aacttgatga cactgacgat cgccgtccga ctcgtgcgca aaaagagcgc aatgaggaac
    26641 cggaaatcga agaagaaatt gacgaatccg aagatgaaac cgtggatgaa gagcgcgtag
    26701 agcgtcgtcc gcgtaagcgc aaaaaagcag ccagtaaacc cgcttctcgt cagtatatga
    26761 tgatgggcgt cggcattctg gttctactgc tgttgatcat cggtatcggt tctgcgctaa
    26821 aagccccctc gacctcttcc agcgatcaaa ccgcgtctgg cgagaagagt attgatcttg
    26881 caggcaatgc gaccgatcag gcgaatgggg tgcagccagc gccgggaacc acgtctgcgg
    26941 aaaatactca gcaggatgtt tctctgccac cgatctcttc tacgccgact caagggcaaa
    27001 ccccggcggc aacggatggt caacaacgtg ttgaagtgca gggtgacctg aacaatgcgc
    27061 tgacccagcc acaaaatcag caacagttga acaatgtggc ggtcaattcc acattgccga
    27121 ccgaacccgc gactgtcgcg cctgttcgca atggcaatgc atcgcgtgac acggcgaaaa
    27181 cgcaaaccgc tgaacgtccg tccactacgc gcccagctcg tcagcaggcg gtgattgaac
    27241 cgaaaaaacc gcaagcaacc gtgaaaacgg agccgaagcc ggtagcacag acgccgaagc
    27301 gtactgaacc agctgcccct gtggcgagca cgaaggcacc gactgcgact tctacgccag
    27361 caccaaaaga gacggcgact acggctccag tacagacggc atccccggcg caaaccacgg
    27421 caacaccagc cgctggaggg aagaccgcag gtaatgttgg ttcgttgaaa tcggcaccgt
    27481 ccagccatta cactctgcag ctgagcagtt cctctaacta cgacaacctg aacggttggg
    27541 cgaagaaaga gaatctgaaa aactacgttg tctatgaaac ggcgcgtaat ggtcagccgt
    27601 ggtatgtcct ggtttctggc gtgtacgctt cgaaagaaga ggcgaaaaaa gcggtatcta
    27661 cattgccagc agatgttcag gccaaaaacc cgtgggcgaa accgctgcgt caggtacagg
    27721 ccgatctgaa gtaatcaagg ttatctcccg caatggttta tcgttgcggg agctgcctga
    27781 agcgctggat gctgtcggag ctttctccac agccggagaa ggtgtaatta gttagtcagc
    27841 atgaagaaaa atcgcgcttt tttgaagtgg gcagggggca agtatcccct gcttgatgat
    27901 attaaacggc atttgcccaa gggcgaatgt ctggttgagc cttttgtagg tgccgggtcg
    27961 gtgtttctca acaccgactt ttctcgttat atccttgccg atatcaatag cgacctgatc
    28021 agtctctata acattgtgaa gatgcgtact gatgagtacg tacaggccgc acgcgagctg
    28081 tttgttcccg aaacaaattg cgccgaggtt tactatcagt tccgcgaaga gttcaacaaa
    28141 agccaggatc cgttccgtcg ggcggtactg tttttatatt tgaaccgcta cggttacaac
    28201 ggcctgtgtc gttacaatct gcgcggtgag tttaacgtgc cgtttggccg ctacaaaaaa
    28261 acctatttcc cggaagcaga gttgtatcac ttcgctgaaa aagcgcagaa tgcctttttc
    28321 tattgtgagt cttacgccga tagcatggcg cgcgcagatg atgcatccgt cgtctattgc
    28381 gatccgcctt atgcaccgct gtctgcgacc gccaacttta cggcgtatca cacaaacagt
    28441 tttacgcttg aacaacaagc gcatctggcg gagatcgccg aaggtctggt tgagcgccat
    28501 attccagtgc tgatctccaa tcacgatacg atgttaacgc gtgagtggta tcagcgcgca
    28561 aaattgcatg tcgtcaaagt tcgacgcagt ataagcagca acggcggcac acgtaaaaag
    28621 gtggacgaac tgctggcttt gtacaaacca ggagtcgttt cacccgcgaa aaaataattc
    28681 tcaaggagaa gcggatgaaa cagtatttga ttgccccctc aattctgtcg gctgattttg
    28741 cccgcctggg tgaagatacc gcaaaagccc tggcagctgg cgctgatgtc gtgcattttg
    28801 acgtcatgga taaccactat gttcccaatc tgacgattgg gccaatggtg ctgaaatcct
    28861 tgcgtaacta tggcattacc gcccctatcg acgtacatct gatggtgaaa cccgtcgatc
    28921 gcattgtgcc tgatttcgct gccgctggtg ccagcatcat tacctttcat ccagaagcct
    28981 ccgagcatgt tgaccgcacg ctgcaactga ttaaagaaaa tggctgtaaa gcgggtctgg
    29041 tatttaaccc ggcgacacct ctgagctatc tggattacgt gatggataag ctggatgtga
    29101 tcctgctgat gtccgtcaat cctggtttcg gcggtcagtc tttcattcct caaacactgg
    29161 ataaactgcg cgaagtacgt cgccgtatcg acgagtctgg ctttgacatt cgtctggaag
    29221 tggacggtgg cgtgaaggtg aacaacattg gcgaaatcgc tgcggcgggc gcggatatgt
    29281 tcgtcgccgg ttcggcaatc ttcgaccagc cagactacaa aaaagtcatt gatgaaatgc
    29341 gcagtgaact ggcaaaggta agtcatgaat aagtttgaag atattcgcgg cgtcgctttt
    29401 gatcttgatg gtacgctggt cgacagtgct cccggtcttg ctgctgcggt agatatggcg
    29461 ctgtatgcgc tggagttgcc catcgcaggt gaagaacgcg ttattacctg gattggtaac
    29521 ggcgcagatg ttctgatgga gcgcgcattg acctgggcgc gtcaggaacg tgcgactcag
    29581 cgtaaaacaa tgggtaaacc gcccgttgat gacgacattc cggcagaaga acaggtacgt
    29641 attctgcgta aactgttcga tcgctactat ggcgaggttg ccgaagaggg gacgtttttg
    29701 ttcccgcacg ttgccgatac gttgggcgcg ttgcaggcta aaggcctgcc gctaggcctg
    29761 gtcaccaaca aaccgacgcc gttcgtcgca ccgctgctcg aagccttaga tattgccaaa
    29821 tactttagcg tggttatcgg cggcgatgat gtgcaaaaca aaaaaccgca tccggacccg
    29881 ctgttactgg tggctgagcg gatgggaatt gccccacaac agatgctgtt tgtcggcgac
    29941 tcacgcaatg atattcaggc ggcaaaagcg gcaggttgcc catcagttgg cttaacctac
    30001 ggatataact acggcgaggc tatcgatctc agccagcctg atgtaattta tcagtctata
    30061 aatgaccttc tgcccgcatt agggcttccg catagcgaaa atcaggaatc gaaaaatgac
    30121 taagcccatc gtttttagtg gcgcacagcc ctcaggtgaa ttgaccattg gtaactacat
    30181 gggtgcgctg cgtcagtggg taaacatgca ggatgactac cattgcattt actgtatcgt
    30241 tgaccaacac gcgatcaccg tgcgccagga tgcacagaag ctgcgtaaag cgacgctgga
    30301 tacgctggcc ttgtatctgg cttgtggtat cgatcctgag aaaagcacca tttttgttca
    30361 gtcccacgta ccagaacatg cgcagttagg ctgggcactg aactgttata cctacttcgg
    30421 cgaactgagt cgcatgacgc agtttaaaga taaatctgcg cgttatgccg agaacatcaa
    30481 cgctggtctg tttgactatc cggtgctgat ggctgcggac atcctgctgt atcaaactaa
    30541 tctggtaccg gtgggtgaag accagaaaca gcacctggaa ctgagtcgcg atatcgccca
    30601 gcgtttcaac gcgctgtatg gcgagatctt taaggtgccg gagccgttta ttccgaaatc
    30661 tggcgcgcgc gtaatgtcgc tgctggagcc gaccaagaag atgtccaagt ctgacgataa
    30721 ccgcaataac gttatcggcc tgctggaaga tccgaaatcg gtagtgaaga aaatcaaacg
    30781 cgcggtcact gactccgacg agccgccggt agttcgctac gatgtgcaga acaaagcggg
    30841 cgtttccaac ctgctggata tcctttctgc ggtaacgggc cagagcatcc cagaactgga
    30901 aaaacagttc gaaggcaaga tgtatggtca tctgaaaggt gaagtggctg atgccgtttc
    30961 cggtatgctg actgaattgc aggaacgcta tcaccgtttc cgcaacgatg aagccttcct
    31021 gcaacaggtg atgaaagacg gcgcagaaaa agccagtgta cacgcttccc gtacgctgaa
    31081 agcggtgtac gaagcgattg gttttgtggc gaagccgtaa tttaacatta atgccaaaaa
    31141 ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg tgaagcgcct
    31201 cgccgtttcc acatctcgtc gttgccactt atcttcattt taatgaagat ataaatattc
    31261 attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg tcgtcatcac
    31321 gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat cgactgaaaa ccatagatga
    31381 gctggcaact gaatgccgtt catcggttgg cctgacgcag gccgcgttga aaacgttgga
    31441 atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc tggtcgagat
    31501 ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg cgatgccttt
    31561 gccctatacc cgcttgtacg aaggtttagc gagcgggctg aaagctcagt ttgatggcat
    31621 tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt tacttaacgg
    31681 cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca cgcaaaaagg
    31741 gttatgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc agttgatttg
    31801 ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt cggcggatca
    31861 gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg atctctctta
    31921 tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct ggaacgtggt
    31981 ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag acgatccgcg
    32041 atttttacag gccaccgaag ccgtggtcct gacgcgagtc gatgattacc caatgcaaca
    32101 actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac gggtagtgag
    32161 tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc agactcaacc
    32221 tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg caggtcgtca
    32281 acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg atggcgatga
    32341 cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag ccgctggata
    32401 acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg caattgcatc
    32461 aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc tatttgctgg
    32521 ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc cgacaaatcc
    32581 acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac agaaacgagg
    32641 caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt ccgccgcact
    32701 aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg tggatcaggt
    32761 actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg aactgtatct
    32821 gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg cattaggtta
    32881 cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg ctggtttgtc
    32941 ggtggcacat caggggcatc tggtacaaat cccttgtcat caggttgctg acgccgttga
    33001 acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag tttctgcggc
    33061 agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg acgatgattt
    33121 tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg tggttgctga
    33181 aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt gcctgctttg
    33241 ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga tacaggcacg
    33301 ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt cagcgaccag
    33361 ctgcacttcg ctgccattac tggcgtaata cggtgtgact catgccgaac ccggtcatgc
    33421 gctgacgggc actattccgg caaaccagca gggcgatcag tctgaacgta tcgcgatgct
    33481 ctggttgagt gaaatctccc atcatttccg tggcgacagc tactgctacg gcggcggtta
    33541 ctaccgtcgt ggtcatgcgc aacatgcgtt ggtgtttacg ccagaaaatc aaaagattac
    33601 tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgt cgctggcagg
    33661 cgagtttccg gtaagcagtg cagtggtgct ctgtttccgc acgcagattt ttgtcacccg
    33721 tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccgaaaatcg ttggtcgtta
    33781 tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt agtgattgat
    33841 agctttggcg taggggcaat gaaagatgtc acgctggtgc gtccgcaaga tgcgggagcg
    33901 aatacatgtg gtcacatcct gagccagttg ccgcatttgc agctaccaac gctggagaag
    33961 ctggggctaa tcaacgcatt gggttatgcg ccaggcgata tgcagccgtc agattccgca
    34021 acctggggcg tggcagagct gcaacatgaa ggtggcgata cctttatggg gcatcaggaa
    34081 attttaggca cgcgcccgtt accgccgctg cggatgcctt ttcgcgatgt gattgaccgt
    34141 gttgagcagg cattagtatc cgccggttgg caggtggagc gccgtggcga tgaactgtaa
    34201 tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga tttaggccaa
    34261 gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat caaaattggt
    34321 cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg cctgttaacc
    34381 gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat tggtatcaat
    34441 gcgccgcgtt ctggcgctta tgacaacggc ttccaggtcg tgcatatggg ctacggtgtc
    34501 gataaaaaag tgcaggtgcc gcaaaaactg tatgaggcgg gcgtgccaac cgtgctggtg
    34561 ggtaaggtgg cagatatcgt cagcaatcct tatggcgtga gctggcaaaa cctggtggat
    34621 agccagcgga ttatggatat caccctcgac gaatttaaca cccatccgac ggcgtttatt
    34681 tgcaccaata tccaggaaac cgacctcgct ggtcatgcag aagacgtcgc acgttatgcc
    34741 gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgatgcgat gcagccagat
    34801 gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca cagccaccat
    34861 acccgcgaag tggtgccgct gctggtttat cagcaaggcc tggctgctac gcagctcggt
    34921 gtgcgcacca cgctttctga tgtgggagct accgtgtgtg aatttttccg tgcgccaccg
    34981 ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac cctatgagtt
    35041 ttgatccgac gggttacacc ctggcccatg agcatctgca tattgatctc tccggcttta
    35101 aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag atgaacgacc
    35161 tgatgacccg gggtgtacgt aatgtgattg agatgaccaa ccgttacatg gggcgcaatg
    35221 cgcaatttat gctcggtgta atgcgcgaaa ccgggatcaa cgtggtggcc tgtactggtt
    35281 attaccagga tgcgtttttc ccggaacatg tggtgacccg cagcgtgcag gaactggcgc
    35341 aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa gccgggatca
    35401 tcgcggagat cggctccagc gaaggaaaga ttacgccgct ggaagagaag gtatttattg
    35461 ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg tcgttcagca
    35521 ccatggggct ggaacaactg gctctgttgc aagcccacgg agttgatctt tcgcgcgtca
    35581 ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg atcgatctcg
    35641 gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac gaaaagcgca
    35701 ttgcgatgct ccacgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg ctgtcgatgg
    35761 atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac tatttattga
    35821 ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg gatgtgatgt
    35881 tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa gattggcgtt
    35941 gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctac tggcagtttt
    36001 gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg gcaactggat
    36061 tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc catcgcggtg
    36121 attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa agacgagcat
    36181 atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga gcacgtcgaa
    36241 cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg gatctgtata
    36301 ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg catcgctcgg
    36361 cggctgtttt tcatgacggt atccgcccga tcctgccgca actgattgaa ggctatatga
    36421 accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg gcctcggtgg
    36481 ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt cttcctaccg
    36541 atatcctcgg cgtactggcg ataaacagcc tgatggcgtt tggtcttggc gctatctggg
    36601 gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg ctgccggtgg
    36661 atgtattagg tagcctcggg gaattaagct cgccggtggt ttctgctttt gcactgttcc
    36721 cgctggtggc gattttctac cagtttggct ggaagcaaag tctggtcgcc gccgtggttg
    36781 ttctgatgac ccgtgtggta gtcatgcgct atttcccaca tcttaaccct gaatccatcg
    36841 aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac ctgcgtcatc
    36901 gtgatgaaaa tgatattgat gccagcgggc tttcggtgtt tgaagaacgc acgtcgcgga
    36961 ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc gttgccagca
    37021 tgaagatttt cgccggcagt gaagtgtcga tcttcactct ggagaaagcg tactccgcag
    37081 gcgtaacgcc ggaacaatcg caaacgctga tcaaccaagc ggcactggca gaatttatgc
    37141 gcgggctggg ttttgtaccg ttgattgcca ccaccgcctt agcaacgggt gtgtatgcag
    37201 ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg atagttgcag
    37261 cggtattagg cgcagtggtt atttcggcgg aagtcttgct gcttcgttcg atcggcaaat
    37321 ggctgggacg ctacccgtcg gtgcgtaatg cgtcggataa catccgtaac gccatgaata
    37381 tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag atggcgggtt
    37441 ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg ggccgtccgg
    37501 tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg ctgaatgttc
    37561 tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga cgtttcctct
    37621 gcaaagtctg acgattattg aggcgcagca aaagcagttt gcgctggtgg atagcatttg
    37681 tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa cgccaggact
    37741 gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat ttcacgcaca
    37801 ggctgcggcg ctggtgcagg gtgcgggtac tggcgcgatt cgtgcggcgt tggcggcttt
    37861 gctcaaaccg gggcagcgtc ttctggtaca tgatgcgcct gtttacccga cgacacaggt
    37921 cattattgag cagatggggc tgacgcttat tactgctgat ttcaatgacc tgtcggcact
    37981 gaagcaggtc gtcgacgagc aactaccgga tgcggcgctg gtgcagcata cgcgccagca
    38041 gccgcaggac ggctacattc tggcagatgt gctggcaaca ttgcgctcgg ctggcgttcc
    38101 tgcattaacc gatgacaact atgcggtgat gaaagtggcg cgaattggct gcgaatgcgg
    38161 ggcaaatgtc tcgacttttt cctgcttcaa gctgtttggg ccggagggtg ttggcgcggt
    38221 ggtaggcgat gccgatgtta ttagtcggat tcgctccacg ctctattccg gcggcagcca
    38281 gattcagggc gctcaggcgc tggaagtctt gtgtgggctg gtgtttgcgc cagtgatgca
    38341 cgcggtgcag gcgggggtat ctgaacggtt gctggctttg cttaacggtg gtgcggtgcc
    38401 ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg tcgagtttca
    38461 tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct tgccttaccc
    38521 ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt ccggaacgtt
    38581 tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga atcgcaacgg
    38641 tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat gctggatagc
    38701 accgtcaagc taaattccgt actgaacggt cccctcgccc ctttggggag agggttaggg
    38761 tgaggggaac gtacgggatg taaaattcaa cgcgccagca tcgccggatg cgatgctggc
    38821 gcatcttatc cggcctacgg ttaatgtctt ttattatcaa tcgttaaggt gatgcgattg
    38881 gcgcggcaga agagttcgga gacgtgcacc ggtttgtcat tctgatcaaa ggcgattttg
    38941 ctgatgcgaa acaacggttc gcccagttcg catttcagcc attttgcctg cgggcgggtg
    39001 gcggcaaaga tatcgatggt ctttttgtcg ctgaccactc gcgtgtcaaa acgctcctga
    39061 aataactgat aggtggagct tccttcgacg taaatctcat caaagtcagg ataacgcgac
    39121 agcgggatcc atgaactatc aataaacagc ggctctttat cgagatacat cacccggcag
    39181 agatggaaca cttcgctgtt tccggggatg ttcagctttt cgcaaaacgg cgcggcgctg
    39241 acccgttcct gttcgatcac tttctctttc gtcgccttgc cttgtgagac gccaaaatcg
    39301 gtaaaaccac tgacagtaag cagggcgttt tcaactttct ggctttgtac aaaggtgcct
    39361 ttgccctgcc agcggatcag tacgccgtct gccactaagt cgctgatagc tttgcgaata
    39421 gtaatgcggc tgacgttata ttgcgtacaa agctcgtttt cggtagggat ctgttgcccg
    39481 gcctggtaaa ccccctgcgc gatatcatcc agcagccgct ggcggacggt agcgtagagg
    39541 agttgatgag agtagcggtc cgtagctgac ataatcgacc ctgaaataaa gaatgaatta
    39601 cacggattat agggtcgttt tccggcaagt aacagcacgt tggcgcggag atgctcacgc
    39661 caacgttata cctaccaggc accgtgatac tgaatggttt tcgccgcgca cgccgttccc
    39721 tgcgccatcg cctgcggtaa tgtcatcccc gcagaccagc cgcaaaggaa tccggcaatg
    39781 aacgaatctc cggcacccat ggtgtcgata accgtcaccg gttcaggagc ctgacgccag
    39841 aactgcgcgc catcccaggc aatgctgccg ttttcaccca gcgtgacaat cactgttcct
    39901 gcgccacggg caacaatcgc tttcatcttc agacgcagcg cttcgtcttc ttgcggtgcg
    39961 gaggcaaagg caaaatcgag atgcggcacc agtgtctgcc agagcgggct gtcccacttg
    40021 tcggagaagt cgaaagcggt aagtttgccc gcagcgtgca gctgtgggaa tgcgtcttcc
    40081 gcatgtcccc agattgccgc gtgcacaatg tcatactgcg ccagccaggc gtaatcctct
    40141 tcactcaggg caaagtcggc catcacgcct tcggtgtagt cgccaaaaac gcgatcattg
    40201 tcgtgcagtt ccacctgagt ttgtgcggta acgccgtgtt tcgtatggac atggctgata
    40261 tcgacgccca tgcgggcgag atcctgcttc agctttgtgc cgtagtcatc gtcacccacc
    40321 aaggtaatgc atcccggctg tatgccgtag cgagtgcagt acaccgccac attgaccgca
    40381 ttaccgccag aaaacgcttt attcagttgc gggtagatat cgacgcagtt atcgccgatt
    40441 gtcgccaggg ttttcatctc tcatcctccg gcagcagcgc gcgaaagcgt tccagcgcct
    40501 ggcgggcata gagtctgggc tcgttcatat acatcgtcac cagctccacc gtacagtaac
    40561 cctcatagcc ccgctcaata atatcgcgca tcagttcccg cagcggcatt ttgccttctc
    40621 caggaatgta atgcgtgtcg ctggccccgt cgctgtcgac aatatgcaga tgacgataat
    40681 ttatcgccca gtttgtcgaa ataactcatc accggttccg cctggacata cggcgcgcaa
    40741 atgtcgacca tgctgaacag gcgcggcgaa ggcaccagcg ccagcgcatg aagcacatca
    40801 ttagcattac acacgacgtt cgattcatac ggcgttaacg gttcgaggat tagatccatg
    40861 ccgatgtttt ccgcgtactc acacagttcg cttaggttct ctgccagccg tccccagata
    40921 acattaggtg gcgtgagata gcctgcgtgg gccgcggaaa tcagcgtata acccgcgttc
    40981 atctcttttg ccatatccat cgccagcttg atcatgtcga ggctttcgcg acgcatatgt
    41041 tcatcgccca gcatcatgtt atacggatag ccgttggttt ctggcgtata gccgataatc
    41101 ggcatctgat acgtctgcgc cagcgccttg atttgtttga tgccgcccgc ttttaagtcc
    41161 ggcgcgaacg cgtgcgggcg accgccccaa atttcgatgc cgtcgtaacc cagctcgctt
    41221 gcatcacgaa atgcgtgttc aataggcagc cgctggtggc cgcaggtaaa catacctgtt
    41281 ttcatcgggt aactccttgc gtaaatccgg cccgttgccg ggccgggaga gggattaata
    41341 ttccaccaga ccaccgtaat agcggcgttc atccgggttg tgatctttgt aaatagacag
    41401 gtagtagcag agccactcca ttggcacgaa catcaggaac ggtgccagcc acgggtgcag
    41461 cccttgcgaa atttcggcgt aatcgatgac gatcacgttg tcagtacgct gtttaacaaa
    41521 gttaatggcg cgttcggtgg tgtggcgact ttcatcattg ccgagcagga acaggaacgg
    41581 aacgcccggt tcgacaatct ccagcgggcc atggcggaac tctccgctct caatcacgca
    41641 gccgtgcgtc caggtaaatt ccatcagcgt tacaatgcct tctttgtaac ccagcggacg
    41701 cagcggaccc gcagcaacgg tataaatcat cggccactgg ctggccagtt caccaagctg
    41761 gcggcctttt tcttcccagg tgcgtaccag atgaccgagc gcattcggca acggcttgag
    41821 atcgtttttg atcttgccga tttccgcgtt cggcgcgagg cgggtgatca tctccagcac
    41881 cacgctgtag cagagcagca ggtgaatttc ccagatacag tcggcctgat aatcaatgct
    41941 aaattccgcc gccgaggtaa tcgggctgtc cgcgcgtttg gtgaacgccg cagtgagtgc
    42001 gccgcaggcc cggcccagct ccagcgcttt gattacctct tccgttttac cgtagtcaga
    42061 aacgccaatc accgcgcaac gatcgtcgag gcgatacggg gtgttatcgc agaactccca
    42121 gccggaaatg gcgtagacct gaagatcgga aaagcgatcc gccagatgtt tcgccgtttg
    42181 cgccgcgttg agtggcgagc cgcaggcaac gaaataaata cgatcaatgt cgcgcttcac
    42241 catctcttcc acgatggcgt gcaccagcgg aacgtcatgg ctgagaactt tttccacttc
    42301 ctgaaccata ttttcggtga ccagaaagtc cacggtgctt ttatcaatat ccaacatttt
    42361 attttctcca gataacatta cgacaaagcg ttgagctggc ggctacgctt cgcccagaag
    42421 gcgtaagcag gcagaccagt agcaataacg atgacggcgc agataaggcc gggaatcggt
    42481 gcccagacaa aggttgaggc gaccagaatg aggcttgacg caatggcgag ggtggtcatc
    42541 agcccgaaag ccggagtacg ccacagcggt ttgtaatcgt cgcgtttacg acaccagatg
    42601 atggagccga aggtgagggt atttttgaaa cacatcacca gggtgaaata acccagcagg
    42661 ctggtgagat cggaaacgaa gatgaagaag atccccagcg ccccttgcag gatgatggag
    42721 acatccggcg tgttgtattt cggatgcaca tggccgaagc atttaaagaa caggttgtct
    42781 ttcgccatcg cgtattccag ccgcggctgg tacatcacgc agctggaaag cgaaccaaga
    42841 atgacgatca tcgccgtgat ggcaacaaag atcccagcgg tgctgccgag tgcggggatc
    42901 caggtcaggg cgtcggaaat cggcgtttca gaattggcga gtttgtcgaa gggcatcagg
    42961 ccggaaatca ccagcgccag cagggtgtag agcaccagaa ccagcagaca ggaaccaatc
    43021 agcgctcgtg gcatggtttt tccggggttt ttaatttcgc cggtcatata acagatagag
    43081 gccatgccgg tatacgacca actggtggca gagatccccg ccagcagcgc cataaagctg
    43141 cccgttgcgc caatcgcagt ggtggtaggg gcggcaaaat tctccgcttt aaaccagaag
    43201 atcccaaggc caatgacgat agtgaacggg ataattttgg cgatggtaat tagcgtctga
    43261 aacgctgcgc cgccttcaac ggagcgcagg tgtagcaaca taaaggcgat aattaatccg
    43321 gcggcgataa atttaccgag caacggatcg ataggcgtta aaaagccaag attgctgaca
    43381 atcgccagcg ccataatcga caatgacggc gcatcgttgg cccagaagct ggcccagccg
    43441 gagaggaaag ccagcggtcg gcttccggca tttttcagat aaacataatc tgcgccattt
    43501 tccggataag cggtggatag ttccgcatag acgcacattt gcgggatcac aattaacccg
    43561 ccaatgacaa acgcgagcac cgtaagccac ggcgtgcccg ctgcttttgc cacttcaccc
    43621 acagatacaa aaataccgga gccgacggtt gtcccgacgg cgattgcaag aacggcccaa
    43681 aatccgagct tgcgttggag ttcctggctt ccc
//
LOCUS       DAEEKQ010000014        43275 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.014, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000014 DAEEKQ010000000
VERSION     DAEEKQ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 43275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43275
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.014"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            58..576
                     /locus_tag="KLK57_000796"
     CDS             58..576
                     /locus_tag="KLK57_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HBE0891251.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            592..1131
                     /locus_tag="KLK57_000797"
     CDS             592..1131
                     /locus_tag="KLK57_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HBE0891252.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLTLAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1226..2803)
                     /gene="guaA"
                     /locus_tag="KLK57_000798"
     CDS             complement(1226..2803)
                     /gene="guaA"
                     /locus_tag="KLK57_000798"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HBE0891253.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWARLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2872..4338)
                     /gene="guaB"
                     /locus_tag="KLK57_000799"
     CDS             complement(2872..4338)
                     /gene="guaB"
                     /locus_tag="KLK57_000799"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HBE0891254.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4500..5870
                     /gene="xseA"
                     /locus_tag="KLK57_000800"
     CDS             4500..5870
                     /gene="xseA"
                     /locus_tag="KLK57_000800"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HBE0891255.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5867..6082)
                     /locus_tag="KLK57_000801"
     CDS             complement(5867..6082)
                     /locus_tag="KLK57_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HBE0891256.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6151..7623)
                     /gene="der"
                     /locus_tag="KLK57_000802"
     CDS             complement(6151..7623)
                     /gene="der"
                     /locus_tag="KLK57_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HBE0891257.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLERVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDTNVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7741..8919)
                     /gene="bamB"
                     /locus_tag="KLK57_000803"
     CDS             complement(7741..8919)
                     /gene="bamB"
                     /locus_tag="KLK57_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HBE0891258.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8930..9550)
                     /gene="yfgM"
                     /locus_tag="KLK57_000804"
     CDS             complement(8930..9550)
                     /gene="yfgM"
                     /locus_tag="KLK57_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HBE0891259.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGKALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9568..10842)
                     /gene="hisS"
                     /locus_tag="KLK57_000805"
     CDS             complement(9568..10842)
                     /gene="hisS"
                     /locus_tag="KLK57_000805"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HBE0891260.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10953..12071)
                     /gene="ispG"
                     /locus_tag="KLK57_000806"
                     /gene_synonym="gcpE"
     CDS             complement(10953..12071)
                     /gene="ispG"
                     /locus_tag="KLK57_000806"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HBE0891261.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12098..13111)
                     /gene="rodZ"
                     /locus_tag="KLK57_000807"
     CDS             complement(12098..13111)
                     /gene="rodZ"
                     /locus_tag="KLK57_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HBE0891262.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13396..14550)
                     /gene="trmG/rlmN"
                     /locus_tag="KLK57_000808"
     CDS             complement(13396..14550)
                     /gene="trmG/rlmN"
                     /locus_tag="KLK57_000808"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HBE0891263.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14700..15131)
                     /gene="ndk"
                     /locus_tag="KLK57_000809"
     CDS             complement(14700..15131)
                     /gene="ndk"
                     /locus_tag="KLK57_000809"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HBE0891264.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15280..17592)
                     /gene="pbpC"
                     /locus_tag="KLK57_000810"
     CDS             complement(15280..17592)
                     /gene="pbpC"
                     /locus_tag="KLK57_000810"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HBE0891265.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEG
                     INGIRFPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17593..22554)
                     /gene="yfhM"
                     /locus_tag="KLK57_000811"
     CDS             complement(17593..22554)
                     /gene="yfhM"
                     /locus_tag="KLK57_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HBE0891266.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPDQPIKLDVIKPDGQVLRNVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPNDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGLRPQFALK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLQADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVPAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LSVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGIALKTMGDATRGEEAIALALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKAQNSNLNSDQLVTLQVSNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     VGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22761..23606
                     /gene="sseA"
                     /locus_tag="KLK57_000812"
     CDS             22761..23606
                     /gene="sseA"
                     /locus_tag="KLK57_000812"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HBE0891267.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(24099..24875)
                     /gene="sseB"
                     /locus_tag="KLK57_000813"
     CDS             complement(24099..24875)
                     /gene="sseB"
                     /locus_tag="KLK57_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HBE0891268.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25017..26300)
                     /gene="pepB"
                     /locus_tag="KLK57_000814"
     CDS             complement(25017..26300)
                     /gene="pepB"
                     /locus_tag="KLK57_000814"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HBE0891269.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26478..26678)
                     /gene="iscX"
                     /locus_tag="KLK57_000815"
     CDS             complement(26478..26678)
                     /gene="iscX"
                     /locus_tag="KLK57_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HBE0891270.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26690..27025)
                     /gene="fdx"
                     /locus_tag="KLK57_000816"
     CDS             complement(26690..27025)
                     /gene="fdx"
                     /locus_tag="KLK57_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HBE0891271.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27027..28877)
                     /gene="hscA"
                     /locus_tag="KLK57_000817"
     CDS             complement(27027..28877)
                     /gene="hscA"
                     /locus_tag="KLK57_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HBE0891272.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28894..29409)
                     /gene="hscB"
                     /locus_tag="KLK57_000818"
     CDS             complement(28894..29409)
                     /gene="hscB"
                     /locus_tag="KLK57_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HBE0891273.1"
                     /translation="MDYFTFFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29505..29828)
                     /gene="iscA"
                     /locus_tag="KLK57_000819"
     CDS             complement(29505..29828)
                     /gene="iscA"
                     /locus_tag="KLK57_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HBE0891274.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29845..30231)
                     /gene="iscU"
                     /locus_tag="KLK57_000820"
     CDS             complement(29845..30231)
                     /gene="iscU"
                     /locus_tag="KLK57_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HBE0891275.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30259..31473)
                     /gene="iscS"
                     /locus_tag="KLK57_000821"
     CDS             complement(30259..31473)
                     /gene="iscS"
                     /locus_tag="KLK57_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HBE0891276.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31714..32202)
                     /gene="iscR"
                     /locus_tag="KLK57_000822"
     CDS             complement(31714..32202)
                     /gene="iscR"
                     /locus_tag="KLK57_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HBE0891277.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32503..33240)
                     /gene="trmJ"
                     /locus_tag="KLK57_000823"
     CDS             complement(32503..33240)
                     /gene="trmJ"
                     /locus_tag="KLK57_000823"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HBE0891278.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33359..34162
                     /gene="suhB"
                     /locus_tag="KLK57_000824"
     CDS             33359..34162
                     /gene="suhB"
                     /locus_tag="KLK57_000824"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HBE0891279.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYSQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34307..35161
                     /locus_tag="KLK57_000825"
     CDS             34307..35161
                     /locus_tag="KLK57_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0891280.1"
                     /translation="MALPVNKRVLKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILAVIGQDDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKETKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35352..36632
                     /gene="csiE"
                     /locus_tag="KLK57_000826"
     CDS             35352..36632
                     /gene="csiE"
                     /locus_tag="KLK57_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HBE0891281.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36624..37763)
                     /locus_tag="KLK57_000827"
     CDS             complement(36624..37763)
                     /locus_tag="KLK57_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HBE0891282.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAVRQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37923..38813)
                     /gene="hcaR"
                     /locus_tag="KLK57_000828"
                     /pseudo
     CDS             complement(37923..38813)
                     /gene="hcaR"
                     /locus_tag="KLK57_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
     gene            38949..40310
                     /locus_tag="KLK57_000829"
     CDS             38949..40310
                     /locus_tag="KLK57_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HBE0891283.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40307..40825
                     /locus_tag="KLK57_000830"
     CDS             40307..40825
                     /locus_tag="KLK57_000830"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HBE0891284.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLEQLDKEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40825..41145
                     /locus_tag="KLK57_000831"
     CDS             40825..41145
                     /locus_tag="KLK57_000831"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HBE0891285.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41142..41954
                     /gene="hcaB"
                     /locus_tag="KLK57_000832"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41142..41954
                     /gene="hcaB"
                     /locus_tag="KLK57_000832"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HBE0891286.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAARYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41964..43166
                     /locus_tag="KLK57_000833"
     CDS             41964..43166
                     /locus_tag="KLK57_000833"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HBE0891287.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIAIGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
BASE COUNT        10266 a        11950 c        10859 g        10200 t
ORIGIN      
        1 ttcctctttt gatgatctta aatgtcttat ttttcgtaat gtgtataaca aggaatagtg
       61 atgaaattta aaaaatgtct tctgcctgtg gcaatgttag cgtcattcac tctggcagga
      121 tgccagtcaa atgctgacga tcatgccgcc gatgtttatc aaaccgatca actgaatacc
      181 aaacaagaaa ctaaaaccgt taatattatt tccattcttc ccgcaaaagt tgccgtagac
      241 aactcccaaa ataaacggaa cgcacaagcc ttcggcgcgc ttattggcgc agtcgctggc
      301 ggtgttatcg gccacaacgt cggttctggc agcaattccg gaacgacggc aggggcagtt
      361 ggcggcggag ctgtaggcgc ggcagcgggt tctatggtga atgataaaac cttagtggaa
      421 ggtgtttctt taacatataa ggaaggcacc aaagtgtata cctccaccca ggtgggtaaa
      481 gagtgccagt ttacgacagg tttagccgtt gttattacca caacgtataa cgaaacgcgt
      541 attcagccaa atactaaatg tcctgaaaag agctaataat caggaggagt catgaagaaa
      601 gtttttcttt gcgccatctt agcctcctta agctatccgg ctatcgcctc atcattgcag
      661 gatcaactct ctgctgtcgc agaagcggaa cagcaaggta aaaatgaaga gcaaaggcag
      721 catgacgaat gggtcgcgga gcgcaacagg gaaatccagc aagagaagca acgtcgcgca
      781 aatgcccagg ccgccgctaa caaaagagcg gcaacggcag cggcaaataa gaaagctcgt
      841 caggataaac tggacgccga agccactgcg gacaaaaaac gcgatcaaag ttatgaagat
      901 gagctacgca gcttagagat tcagaaacaa aaactgacgc tggcgaaaga agaagcccgc
      961 gttaagcgag aaaacgaatt tatcgatcag gaactgaagc acaaagctgc gcaaaccgat
     1021 gtggtgcaat ctgaagctga cgccaacaga aatatgactg aaggcggtcg cgatctgatg
     1081 aaaagcgtgg gcaaagcaga agagaacaaa tcggacagct ggtttaatta atcgatgtta
     1141 gtaacttcaa gcctatgatt cttgaagata aaaaaccctc tgtagtaaca gagggttttg
     1201 ttcattcata gtgcagggat taaaatcatt cccactcaat ggtagctggc ggcttgccgc
     1261 tgatgtcata caccacgcgg gaaataccgt tcacttcatt gataatgcgg ttggaaacgc
     1321 gaccgaggaa atcgtacggc agacgcgccc agtgtgcggt cataaagtcg atggtttcga
     1381 cagcacgcag agagacaacc cagtcatact tacgaccatc gcccattacg ccaacggaac
     1441 gtaccggcag gaacacagtg aacgcctggc tgactttgtc gtacaggtcc gctttacgca
     1501 gttcttcaat gaagatggcg tcagcacggc gcagcaggtc acagtactct ttcttcactt
     1561 cacccagcac acgaacgcca aggcctggtc ccgggaatgg gtgacggtac aacatgtcgt
     1621 acggcaggcc cagctccaga ccaatcttac gcacttcgtc tttgaacagc tctttcagcg
     1681 gttcaaccag gcccatcttc atctctttcg gcaggccgcc cacgttgtgg tgagatttga
     1741 tgacgtgtgc tttaccggtt gcagacgccg cagattcgat aacgtcaggg tagatggtgc
     1801 cctgcgccag ccacttcacg tcttccagtt tcagcgcttc ttcatcgaat acttcaacga
     1861 aaacgcgacc gatgatttta cgttttgctt ccggatcgtt ttcgccagcc agcgctgaca
     1921 ggaagcgatc ttctgccgga acgtgaacaa tgttcagacc aaagtgatcg ccaaacatat
     1981 ccagaacctg ctctgcttcg ttgagacgca gcaggccgtt gtcgacgaat acgcaagtca
     2041 ggtttttacc gatagcgcgg tgcagtagca ttgcggttac ggaggaatcc acgccaccag
     2101 agaggccgag gatgacttta tcgtcgccta cctgctcacg gatgcgggct acagcatcgt
     2161 cgataatttt cgctggcgtc cacagggctt cacactggca gatatcacgc acaaaacgct
     2221 ccagcatgcg cataccctgg cgggtgtgag tcacttccgg gtggaactgt acgccataga
     2281 agcgtttttc ttcgttagcc ataatggcaa acgggcagct ttcggtgctg gctacggtga
     2341 cgaagtcgga cgggatagcg gtaactttat cgccgtggct catccagaca tcgagcagcg
     2401 gtttaccgtc tgcggtcagc gcatcttcga taccgcgaac cagtgcactg tcgtttacga
     2461 cttcaacctg cgcgtagcca aattcacgtt cgttagaggc ttcaacgtga ccgcccaact
     2521 gcattgccat ggtctgcatg ccatagcaaa cgccgaatac cggtacgcct gcttcaaaga
     2581 catactgcgg cgcacgcggg ctgttttctt cagtagtact ttccgggccg ccggaaagaa
     2641 taatgccgct tggattgaag tcacgaattt gtgcttctgt cacatcccac gcccacagtt
     2701 cgcagtaaac acccagctca cgcacgcggc gcgcaaccag ttgagtgtac tgagaaccga
     2761 agtctagaat gaggatgcga tgcttatgaa tgttttccgt cattgacgct tattccgagg
     2821 caagtgaaac agataatata aatcgcccga catgaagtcg ggcgaagaga atcaggagcc
     2881 cagacggtag ttcggggact ctttagtaat ggtcacgtcg tgaacgtggc tttcctgaat
     2941 gcccgcaccg ctgatacgta caaactccgc tttagtacgc agttcgtcga tagtaccaca
     3001 gccggtcaga cccatacagg agcgcaggcc gcccatctgc tggtgaatga tctctttcag
     3061 gcgaccttta taagctacgc gaccttcgat accttccggc accagtttgt cggcagcgtt
     3121 atcgctctgg aaataacggt cagaggaacc tttggacatc gcgcccaggg aacccatacc
     3181 acggtaagat ttgtaagaac ggccctggta gagttcgatt tcacccggag attcttcagt
     3241 acccgccagc atggaaccta ccatcaccgc gcttgcgcca gcggcgatag ctttggcgat
     3301 gtcgccggag aagcgaatac caccatctgc gataaccgga ataccggtgc cttccagggc
     3361 ttcaactgcg tcagcaacgg cagtgatctg cggaacacct acgccagtaa cgatacgagt
     3421 cgtacagata gaaccagggc cgataccgac tttaaccgcg ctgcaaccag cttctgccaa
     3481 agcgcgtgca cctgcagctg ttgccacgtt gccgccgatg atttgcagat ccggatattt
     3541 agcacgggtt tcacggatac gctgcaaaac gccttctgag tgaccgtggg aggagtcgat
     3601 cagcagaacg tcaacgccag cggcaaccag cgcgtcaaca cgctcttcgt tacccgcacc
     3661 tgcgccaact gcagcaccaa cacgcagacg gccttgctcg tctttacagg cgttcggttt
     3721 acgttccgct ttctggaagt ctttcacggt gatcatgccg atcaggtgga attcgtcatc
     3781 aaccaccagc gctttttcaa cgcgtttttc gtgcattttt gccagcacca cttcacgggc
     3841 ttcaccttca cgcacggtga ccagacgctc tttcggcgtc atgtaaacgc taaccggctg
     3901 gttcaggtcg gtaacaaaac gcacgtcacg accggtgata atacccacca gttcgttttc
     3961 ttcggtaacg accggatagc ccgcaaaacc gttacgctcg gtcagttctt tcacttcgcg
     4021 cagcgtcgtg gttggcagaa cagtctgcgg atcagtcacc acaccagatt cgtgtttttt
     4081 cacacggcga acttcttctg cctggcgttc aatggacatg tttttgtgga taaagccgat
     4141 accgccttcc tgagccagag caatagccag gcgcgcttcc gttacggtat ccattgctgc
     4201 ggaaagcata gggatattca gacgaatagt tttcgtcagc tgggtgctga ggtcagcagt
     4261 attcggcaga acggtagaat gagcaggaac gaggagaacg tcgtcaaacg tcagagcttc
     4321 tttagcgata cgtagcatgg gcaatatctc gaccagagtg gttaataaat attgccgcgg
     4381 cattatacag agcgtaaccg attgcatcta cccctttttg caaaaaatgc ttgctatccc
     4441 cgaagggcgg gttactatcg actgaataac ctgctgattt agaatttgat ctcgctcaca
     4501 tgttaccttc tcaatcccct gcaattttta ccgttagtcg cctgaatcaa acggttcgtc
     4561 tgctgcttga gcatgagatg ggacaggttt ggatcagcgg cgaaatctct aatttcacac
     4621 aaccggcttc cggtcactgg tactttacac tcaaagacga caccgcccag gtacgctgcg
     4681 cgatgttccg caacagcaac cgccgggtga ccttccgtcc acagcatgga caacaagttt
     4741 tagttcgcgc caatattacg ctctacgagc cgcgcggtga ctaccagata atcgttgaga
     4801 gtatgcagcc ggccggtgaa gggctgctgc aacagaagta cgaacagctc aaagcgaagt
     4861 tgcaggctga aggtttgttc gatcagcaat acaaaaaacc acttccctcc cctgcgcatt
     4921 gcgttggtgt gatcacctca aaaaccggtg ctgcgctaca tgatattttg catgtgttaa
     4981 aacgtcgcga tccttctcta ccggtgatca tctaccccac cgccgttcag ggcgatgacg
     5041 caccggggca aattgttcgc gccattgaac tggcgaatca gcgcaatgag tgcgacgtgt
     5101 tgatcgttgg gcgcggcggc ggttcgctgg aagatttatg gagttttaac gacgaacgcg
     5161 tagcgcgggc gatttttgcc agccgcattc cggtcgtcag cgccgtcggg catgagacgg
     5221 atgtgaccat tgccgatttt gttgccgatc tgcgtgcgcc aacgccgtct gccgccgctg
     5281 aagtagtgag ccgtaatcag caagagttac tgcgccaggt gcaatcgacc cgtcaacggc
     5341 tggagatggc gatggattat tatctcgcca accgcacgcg tcgctttacg cagatccatc
     5401 accgattaca gcaacagcat ccgcagctcc ggctggcacg ccagcaaacc atgcttgagc
     5461 gcctgcaaaa gcggatgagc tttgcgctgg aaaatcaact taagcgtacc gggcaacagc
     5521 agcagcggtt aacacagcgg ctgaatcagc aaaatccaca gccgaagatt catcgcgcgc
     5581 aaacgcgcat tcagcaactg gaatatcgtt tagcagaaac cctgcgcgta cagcttagcg
     5641 ccacgcgtga acgtttcggt aatgcagtaa cgcacctcga agccgtaagc ccactgtcaa
     5701 cgctggcgcg tggatacagc gttactactg ctactgacgg caatgtactg aaaaaagtga
     5761 agcaagttaa agcgggtgaa atgctaacca cacgtctgga agacggctgg atagaaagtg
     5821 aagttaaaaa catccagcca gtaaaaaaat cgcgtaaaaa ggtgcattaa gccagcacga
     5881 attcaacgcg ttttttcgat atcaagccat gcccgtgctg acagaagtaa tctactgcac
     5941 cgcaggcttt taacacctgc aacggttgat ggcagtctgg acaaagagct ttcatttcaa
     6001 taatttcccc gcaggatggg caacgcgcat ggccgttatc ctgatcaagc acatgctgac
     6061 attgtggaca atgcagttcc atatgaattc ctccggtagc catccctcta cattcataga
     6121 gggaatggca gataaaatac ttacggataa ttatttattt ttcttgatgt gcttcatcag
     6181 acgcttacgt ttacgcatct gggttggcgt cagggtgtta cgcttgttcg catacgggtt
     6241 ttccccttct ttgaactgaa tacgaatcgg cgatcccatt acgtccagcg atttgcggaa
     6301 gtagttcatc aagtagcgct tgtaggaatc aggcaggtct ttcacctgat taccgtgaat
     6361 caccacaatc ggcgggttat aaccaccagc gtgggcatat ttcagcttca cacgacgacc
     6421 gcgtaccagc ggcggttggt gatcttcaac agccatcgtc atgatgcgcg tcagcataga
     6481 ggtccccaca cgacgggtgg agctgtcata cgcttcacgt actgattcaa acaagttacc
     6541 aacaccactg ccgtgcaagg cagagataaa gtgcacacga gcaaaatcga taaagcccag
     6601 acggaagtcc agcgtttctt tcacctgctc tttcacttcc tggctcaggc catcccactt
     6661 attcaccaca atgacaagtg agcgtccact attgagaata aagcccagca gcgagagatc
     6721 ctgatcggaa ataccttcgc gcgcatcaat cactaacatc accacgttgg tatcttcaat
     6781 tgcctgcaac gttttgatta cggagaattt ctctacagca tcggtgattt tgccgcgttt
     6841 acgtacgcca gcggtgtcaa tgagcacata ctcacgtcca tcgcgttcca ttggaatgta
     6901 gatactgtca cgcgtcgtgc caggcatgtc ataaacaaca acgcgctctt caccaagaat
     6961 acggttagtg agtgtagact tacctacgtt cggacgaccc acaatcgcca gtttgatcgg
     7021 cagactttgc gggtcgaagt cgtcttcctc ttcttcttcg ccgttctctt ccgcttcaaa
     7081 ttgcgcccag tattcagcgt cttcgtcgac ttcctcttgc ggtgcgagat cttccatcca
     7141 cggcagcagc acacgctcca gcagacttaa tacgccacga ccgtgagatg cggcgatcgg
     7201 gtagatttca cctaaaccaa gcgagtagaa atcaaccact gcctgatcgg gatccagacc
     7261 gtcagttttg tttgccacca ggaaggtcgg tttttcacga gagcgcagat gtttggcaat
     7321 cgcttcatct gccggcatca agcccgcgcg cgcatccacc ataaacagta cgacgtccgc
     7381 ttcttcaatc gccagcagcg actgttccgc catgcgggtt tctacaccgt cttctgtgcc
     7441 atcaatcccg ccggtatcaa tacagataaa ctcacggcct tcaatttccg cacgaccgta
     7501 cttacggtca cgagtcagac ccgggaaatc cgcaaccagc gcatctcggg tgcgagttag
     7561 acggttaaat aacgtggatt ttcctacgtt agggcgcccg acaagcgcga ccacaggtac
     7621 catgtttaaa gcctcatttt tataaatcat cagacaacgc acgctatatt cgcgtcgttg
     7681 ttaaaaacag gaaaacggcc cctgtccagg agccgttttc aaagtgaacg acagagacga
     7741 ttaacgtgta atagagtaca cggttccgtc ttttgcctgg atcagcagtt tgccgtcagc
     7801 ggcaaccggt tcagtctgga aaccggaact atcaactttt tgctgggcaa cgaaacgacc
     7861 atcttcgacg ttaatccagt gcagataacc ttcactgtca cctaccacca ggttgccatt
     7921 atacagcacc ggcgaagtca gcaggcgatg cagcagatcg ctttgtgtcc acagcgtaac
     7981 gccgccatca atggtcaacg ccatcacccg gtcattttga tcgaccagat agatgcgatt
     8041 gccgtcgacg atgaaatcat tcaccgaacc cagttcgcgt ttccacataa tctgaccact
     8101 gcgcagatca agcgccgtca ggttaccatt ataggccaga gcgaaaacaa cgccgttaac
     8161 aacgacggga gtcgtgtcaa catcgctcag acggtcaatt tcggtagaac cggtcgcctg
     8221 ggaaatacgc tgctgccaaa tcatctggcc ctgttccatc agcactgcgc tgacgcgacc
     8281 attatcgccc cccacgacgg ccgcaccaaa agccgttgcc ggcgcagact cgccacgcaa
     8341 agagagcgaa ggcatatcga ggttaactgt ccatttgaca gcgccgtcag cttcgttcag
     8401 cgcttgtaac tgaccgttac tagtgtggat taacaccaga ccgtcgctga ccaccgggcg
     8461 agaaagtgct tcacccgcga ctttagtttg ccatgccaca gtaccatcgc tggtattcag
     8521 cgcgtaaacc tgcgcctttt cgctgccaat gtagacatgc ccaccagaca cggtcacacc
     8581 gccagaaagt aatgcaggct ctttagagaa ccagccatct ttctcggcca ggctgacaga
     8641 ccagatttct ttgccatcat ccgcattcag cgcttttacc aaaccagcgc ggtccgctgc
     8701 atagacaacg ttgtccgcca gtgccggatg aagattggaa tagaagttgc caatgccgct
     8761 accaacggaa gtgctccacg ccgtggtcgg cgtaaactgg ttttcaacgg ttggcaatgg
     8821 ggacatcttt accacatctt cttcgctgtt aaacagcgaa cagccgctta aaagggtaac
     8881 ggaaagcagt cctggcagca gtaatttacg caattgcatc gggtccctct cagatggaca
     8941 aattattaat tttcatctgc atcatttcgc tcagtgccgg agtaacatcg cttttcacgc
     9001 ctgcttccca tgcactacgc gcaccttgct tatcaccttt gctcaacaat gctttaccac
     9061 gcaggtcggc aacaatggca gcccaccctt cacctttgat ggtatcaagg gttttcagcg
     9121 cggcatcagc ctgcttgagc tgtacctgaa cgcgagcaag acgcagattt atcacggctt
     9181 tgagattttc atcactcgtg tctgccagcc cctgttgtaa ctgggcggca gctttctcta
     9241 gttcattttt gtcaacaaat tgctgcgcaa gttccaaaga agccagcgca ccataagtat
     9301 ttttattttc agcagcaaat ttttccgccg ccgggatgct atccggtttg ccttcgctca
     9361 ctgcggtaac cgcattttga taggcaagag aagcggagcg tgcagaatca acctgatggc
     9421 tgttccagta gcgccagcca atcagtgcgc caacgcccaa aatcacccca acagccagtg
     9481 ctttgccatt ttcagcaaaa aagcgtttaa ccgcttctac ctggtcgttt tcgttctcgt
     9541 aaatttccac gctgtccttc tccttcctta acccagtaac gtgcgcaaat gcgcggctac
     9601 gctatcctgc gcaactgccg tttgctcacc agagcgcaaa tccttcacta ctgctgtgcc
     9661 gttagccact tcagactcac ccagcaccac agcaacgcgg gcaccccatt tatcagcacg
     9721 ggcaaactgt ttcttaaagt tgccgccgcc gtggttggtc atcaatttca cgcccggtaa
     9781 ttcatcacgc agacgctcag ctaatgccat agccgcagat tgtgtatcag cacctgaagc
     9841 caccaggtat atatcgacaa caggatcggc tttaaattcc ggattaacgg cctgtactaa
     9901 caatacaaga cgttcgaggc ccatcgcaaa accgactgcc ggtgttgcac gaccgcccag
     9961 ttgttccacc agaccgtcat aacgaccgcc tgcacacacg gtgccctggg agccgaggct
    10021 gttagtcacc cactcgaaaa cggtacggtt atagtaatcc agaccacgca ccagacgctg
    10081 gtttacggtg taagcgatcc ccgcgctctc aagcagtttg cacagaccgg caaaatgctc
    10141 acgagattcc tcgtccagat aatcacctaa tgccggagcg tcgttgagaa gcgcctgcac
    10201 ttccggattt tttgaatcca gcacgcgcag cgggttagtg tacatgcggc gtttgcagtc
    10261 ttcgtccagc ttttctttat gctgctcaag gaatgccacc agcgcatcgc ggtaattggc
    10321 gcgtgcttcc agcgaaccaa tagagttcag ctcaagagtt acgtgttcga agatacccag
    10381 cgcacgccac cagcgggcag tgagcataat cagttcagcg tcgatatccg gaccttgcag
    10441 accgaaaact tcgcagccca actgatggaa ctgacgataa cgccctttct gcggacgctc
    10501 gtgacggaac atcggcccga tataccacag acgctgttcc tgattgtaca gaagaccatg
    10561 ctcgatgccg gcgcgtacac agcccgccgt cccttcaggg cgcagagtca ggctgtcgcc
    10621 attgcgatcc tcaaaggtgt acatctcttt ttcaaccacg tcggtgactt caccaatcgc
    10681 acgtttgaat agcggggtct gctctacaat cggcaagcgg atttcactgt aaccgtagct
    10741 gccgagcacg tttttcagtg tgccttcaat gcgctgccag atggccgttt cgccaggcag
    10801 gtaatcgttc atgccgcgaa tggcttgaat gttttttgcc acgtttattc tctttctgaa
    10861 tataaaaatg aaccctcaac gcttccctca atgtttcggg agccatgcgg gttcaatcat
    10921 acacgggaag cgaggcgctt cccatcacgt tattattttt caacctgctg aacgtcaatt
    10981 cgacgcgctt cgtccagctg actggctttc gcacgaatgc gtgcttccag ctggtcgatc
    11041 atatcgttgt tgtccagacg gtctttgcgc acgccatctt catagaggcc gcttttcttg
    11101 ttgccgccgg tgacgccgag tgtagaaacc agcgcctcac ctgggccatt caccacgcag
    11161 ccgataatcg aaacgtccat cggagtgatg atatcttcca ggcgttgctc cagcgcgtta
    11221 accgtaccga taacatcaaa ttcctgacgc gaacaggtcg ggcaggcgat gaagttaatc
    11281 cctcgcgaac ggatacgcag cgatttcaaa atatcgaaac cgactttgat ctcttcgacc
    11341 ggatcggccg ccagcgatac gcgcagcgtg tcgccgatgc cttccgacag cagcagacct
    11401 aaaccaatgg cggattttac tgccccgctg cgtgcaccac cggcttcggt gatccccaga
    11461 tgcagcggct gatcgatctg ttttgccagc aaacgataag actcaacagc gaggaagacg
    11521 tcagacgctt tcacgctgac tttgaactga tcgaagttca ggcgatcgag atgatcaaca
    11581 tgacgcatgg cggattccag caacgcctgc ggcgtcggtt cgccatactt ttcttgcaga
    11641 tctttttcca gcgatccggc gttaacgcca atacggatcg gaatgttttt atcgcgcgca
    11701 cagtcaacca ccatgcgaat acgctcttca ttaccgatat tgccagggtt aatacgcaga
    11761 caatcgacgc cgtattccgc tactttcagc gcaatgcgat agtcgaagtg gatgtcagcc
    11821 accagcggca cgttaacccg ctgtttgatg agtttgaacg cttctgccgc gtccatcgtc
    11881 ggtacggaga cacggacgat atcagcgcca acgcgttcca gcgccttgat ttgattgacc
    11941 gttgcttcga catctgtcgt acgcgtattg gtcatggact gtacggcgat gggagcacca
    12001 tcgccaatcg gcacattccc aacgtaaata cgtgttgatt ttctacgttg aattggagcc
    12061 tggttatgca tgaaaaatct cccgcgttac ccgtctgtta ctgcgccggt gattgttcgg
    12121 cattgagggt cagacgcgca acctggttag ttctgataaa acgactcaga tcgacaggtt
    12181 tcccttgata ctggatctgt actgcggctg gcgcaccaat tttcagttta tacggtgcct
    12241 ggccggttaa attcaaatta ccgtctttac gctgcatccc gctaaacaat tttttaccgg
    12301 tagcatcagt gacctccagc cagcaatcgg cagtaaagtt catcaccagc gcattcggat
    12361 cagccgccgg cgtggtcacg ccagcctgat cggttggcaa cggcgcagca ccatctggcg
    12421 ttgttgctgc cgttggtacc ggggtcgcgg cggtatcaac atttgcctgc gaaggcgaaa
    12481 caaccgcatt ctgttgcgga tcaacagctg gtgctggcgc agttacggca ggtgtttgcg
    12541 tgttggttgc ggtagtatcc acagacgctg gcggcgtgga cgttgtagcc gggtctgtag
    12601 ttgtcgacgt atttaacggg acactctgcc cctgctcgct attactgctc agttctgccg
    12661 aagattgatc ggccatagtg gtgatctctt cctgctgagc tttgtggtct tgccaccacc
    12721 aggcaccgct caggccgata accacaaaca acaccagcca ggtgaaggtc atcagccagc
    12781 catcacgttt tttgcggcgt ttaccgaggg aaaaactctg cattggcgca acttttgcag
    12841 cccgtagtgg agcctgcttt tccagccctg gcagcagttc ttcttctgga atatgtacca
    12901 gacgcgcata agagcggata tatccgcgca ggaatgttga agcaagatcg gcgggtgcct
    12961 tatcttcttc aatgtcgcgt accgtggaaa ccttcaggca aagtcgctcg gcaacggcct
    13021 gctgactaag tcctagttgt tcgcgagcat tacgcaggcg agcgccggta gtaagtgctt
    13081 cattttggtc gtgcgtggct tcagtattca ttcgctgcag gtacgtttaa atgagaattg
    13141 agatgccggt gaaccatcat gctcacccac accgcgaaac atccgttaag ttaaccgtta
    13201 tcatacagta taagaccgtc tgtgtgctcg tgacaaagcc cacattcact aaggctaact
    13261 tactgttgca tcgttacata ctgccttaaa gtcagcaaaa acgcaccgtt aatattgacc
    13321 agacaattgc aacttaatac ttcattcata aaacattacg ccacggtaca taaagtaacc
    13381 gtggcgtaat ggctatcaga ccgctttaat gtcgatggct tcaccctgca tccgtttacg
    13441 cagggtacgt ttcgtacggt cgataacatc gcccgccaac tgaccacagg cagcatcgat
    13501 atcatcacca cgagttttac gcacaatagt ggtgaaaccg tagctcatca gcacttttga
    13561 gaaacggtcg atacggctgt tcgagctgcg tccatacggc gcacccggga acgggttcca
    13621 cgggatcagg ttgatcttac acggcgtatc tttcagcagt tccgccagtt ggtgcgcgtg
    13681 ttcagtgccg tcgttaacgt ggtcaagcat cacgtattca atagtgactc ggccctgatt
    13741 ggcgttggat ttctccagat aacggcgcac cgcagcaagg aacgtttcga tattgtactt
    13801 tttgttgatc ggcacaattt cgtcacgaat ttcgtcgttc ggcgcgtgca gggaaattgc
    13861 cagtgcaacg tcgatcatat cgcccagttt atccagcgcc ggaactacac cggaagtaga
    13921 aagcgtgacg cgacgtttag acaggccaaa accgaaatca tcaagcatga tttccatcgc
    13981 cggaacgacg ttgttcaggt tgagcagcgg ctcgcccatg cccatcatca ctacgttagt
    14041 gattggccgc tgaccggtga cttttgctgc gccgacgatt ttcgccgccc gccacacctg
    14101 gccgataatt tccgacaccc gcaggttgcg gttaaagccc tgctgggcgg tggaacagaa
    14161 tttacactcc agcgcacacc ccacctgcga agagacgcag agcgtggcac ggtcgtcttc
    14221 cgggatatac accgtttcga cgcgctgatc gccaacggcg atcgcccatt taatggtgcc
    14281 gtcggatgaa cgctgttctt caaccacttc cggtgcgcgg atctccgcca cctctttcag
    14341 tttgccgcgc aacactttgt tgatgtcggt catctcatca aagttgtcgc agcaatagtg
    14401 atacatccac ttcatcacct gatcggcgcg gaagggtttt tcacctaaat ctttaaaaaa
    14461 ctcccgcatc tgctgacggt tgagatccag caggttgatt tttccatctt tcgtggtgac
    14521 gttttcaggt gtgactaatt gttcagacat atgctattcc ggcctcgtta ttacacgtta
    14581 tggcccctgg agggttgaaa aaagaaacgc cccggtgagc ggctgctcgt ccgggggcgc
    14641 tgcattgtac aaattctggc gcacggatgc cacgtttgca cgcggcattt acgaaattat
    14701 taacgggtgc gcgggcacac ttcgccttcg ccgaagaaat aagcgatttc gcgagcggca
    14761 gattcgacgg aatcagaacc gtgggtaccg ttttcggtca ggctgtcagc gtaatcagcg
    14821 cgcagagtac cggccagtgc gtttgccgga ttggtcgcgc ccagcagatc gcggtgacgc
    14881 tgaacggcgt tttcaccttc cagcacggaa accacgatcg ggccagaggt catgaattca
    14941 accagaccat caaagaacgg ttttccatcg tgttcagcat aaaagccacg agcctgttca
    15001 acggtcaggt gcagcatttt ggtgccgaca attttgaacc ctgcagcttc aaagcgcgca
    15061 aagatattac caatgacgtt ttttgctacc gcgttcggtt tgatgatgga aaaagtacgt
    15121 tcaatagcca tttttacctc tgtaaattgt tctgttgttg tctgtaccag cgtacgagat
    15181 ggcgcggatt ataatgagca acagggccgt tgactattga tgaaggtaac atttttttaa
    15241 aataaaatga gttttagcaa caatcaggga caaaacagac tattgcatga caaatttcac
    15301 tgtcgcgatt tgccccacgt catccatcac cagcaattga taatcacctt tatccgtcaa
    15361 atgcagggtc acgttgcgcc cgcgttcagt taacggttcg ccattcagaa accaccagcg
    15421 ttcacctgcc cctccacttg attgcaacgg caaagtcgct tccgctgcgc ccggtaaacg
    15481 tttaataatc gcgccatcgc gaacgcctgt cagctgcaga gggagctggg catcgtgacc
    15541 gtatggcggg caaattgtcg aggccggtgg taagcgcaca gcgcgacgtt ctgatgcggg
    15601 cagccagggt tccagcggta gcggccagac atttaccatt tcttgcttcg cctgcgggca
    15661 gtcagcggcg acgcgctgcc ctttgtcatc cagccagatg gggaagcgaa tgccattaat
    15721 cccttcctgc tctgacagta acagcgttgg cggctggctg ccatccagca gccaggttgc
    15781 caggcggcgg cgacagttac cgtcaccttc cggcaaagac tgtccgcccg gccagcagat
    15841 aacgccacga gtgactgagt tcgggcgcgg gtcttccggc agattcgcac tgcgcgacag
    15901 taagatatta ttgacctgat tcaacaatgg tacggcactg gcaaagccaa actgaccaac
    15961 aacgggcgtg ccgtcaggtc tgccagtcca gatcccaatg acatagcgag cgttaacccc
    16021 aatcgcccag gcgtcacgat agccatagct ggtgcccgtt ttccatgcca gtggggcgac
    16081 gcgcggcaag gcactatccg gcaagggttg cgcttcatca gccataatcc ggcgaatgat
    16141 ccacgccgcc cccgacgaca ttaaaggccg ttcaagcagc ggatcgtcag gctgtaagcg
    16201 caatttgcct gccttgccgt ggcgagcaaa cgcggtatac gctgccgcca tatcttccag
    16261 ttttgcacca gcgccgccga gaatgagtga aagattcggc gcagcgccgt tgggcaaata
    16321 taacggcaat ccaacattgc gtaactttgc cgcaaaccgt ttcggtccat aggcttccag
    16381 cacctgcaca gcaggtaagt tcagcgagcg caccagcgcc tcgctcatgc tgatcgggcc
    16441 atgaaaaccg ctatcaaagt tacctggtcg ataatcaccg gtgcgccggg ggacgtcttg
    16501 cagcagtgat gccgggtgga tcaagccttc atccagcgcc agaccataaa caaacggttt
    16561 gagcaccgat cctggcgatc ggatcgcatt gaccatatca acatgaccaa agcgtgaatc
    16621 atcgttgaga tcaaccgatc ccacccagcc gcgaacacgc atatcggtat gatcaaccac
    16681 gatcatcgcc agtgagctgc gcggtggcaa ccgccctttc cagttttgcg ccagttcttc
    16741 cagacgtcgt tgaagaccgg catccagcgt agtaacgatt ttgtcgcttt tgcttttacc
    16801 gagcatcatg cgcgaaaaca gcggtgccag ttgcggcatt tgtcggggtg ccagccagat
    16861 gggttcttcc cttgactctt ttacctgctc acgggaccac acaccttgcg cagccatccg
    16921 ttcgagcact ttattacgcg cggcttcggc acgctccggc caacgatccg ggcgaagacg
    16981 gctgggcgct tgcggcaaaa ccgccagcat tgccgcctcg gaatagctta aattcgcagg
    17041 cgactttccg agataagccc aacttgccgc accgatcccc tgcaacgtac cgccaaacgg
    17101 agcgcggtta agatacaagg tcagaatttc acgcttagac agatgccatt ccagttgcaa
    17161 cgcgcgccag agctggcgaa ttttgccgcc aaatgttttg gggtgaggat caagcagacg
    17221 agcaacctgc atagtgagcg tgctgccacc ggaaataacc cgtcccgaag tgagatcttg
    17281 ccatgctgcg cgcgccaccg agaatggatt cacccccgga tgcttccaga accagcgatc
    17341 ttcataattg atcagcgctt caaggtaacg tggagaaaca tcttcgattg ttaccggata
    17401 acgccagatg ccgtcagcat cggcgaagcg ccagagcggc gtaccatcct gcgccacgac
    17461 cactcgtgcg ggattgactt catgcagcgg tagaggccag agtttatctg ctccccacgc
    17521 tgcaagaaaa aggagaaatg gcgcagctgc cagcgttatc cagcagccgc gtttggttaa
    17581 caagcgaggc atttacggtc ggacaatcag cagatcttca gccgcgccgg tcgcccgcca
    17641 ttggggaaca tacattgatt ccaccatcgg ttgcggtacc tgatacgttc ccggcgtcac
    17701 cgcccgcgcc agatacacca gcgttaccgg ttggtattca tcaacggcaa ccgccgccac
    17761 aaagcgatcg tcacggaact caatgtgctt aatactcgcc tgctgcatct ggttcagtaa
    17821 gttctgcact tcgccaccac tttgctccag gctggcgcta ccgttcgcca gattctggtt
    17881 ttccagttcc agacccacag gcagcagatc cacgactaac gcatccggca cgctgttact
    17941 ggcttttacc tgcaaccaca ccagcaccag atcgccgcta cgtaacgagt ccagcgattt
    18001 gctcttacca tcagtaccaa gaatatgacg ctcgatttgc agcacattgt tcgccggtaa
    18061 aggtgcggat tgcggataac cgctggcatc catacgcaac cataacggct gatcgccact
    18121 gttgctcacc tgcaaggtga caagttgatc gctattcaga ttgctgtttt gcgctttctc
    18181 gcctgtcagc ggctcagctg agaaagaggt ttgcgcctgc catttacccg gtaaatcctg
    18241 aatcgtacgg gcagccagga acaacgcgtt actttcctgc gtcgatagcc agcgttcacc
    18301 aaacgcctgc tgcgaaagtg tgttcagcaa agtgtactgc tcatcgggta gcagtttatt
    18361 ttcttccagc aaggagagca ttaacgcgtt gtcgcgcagt ggactaccgt aatcacccag
    18421 ccatatccgc tcatcactat ttctcggcgt tttcagcgcc agcgcaatcg cttcttcacc
    18481 acgcgtcgca tcacccatgg ttttcagcgc aatgccaagt tgcagcagcg gtaaaccaga
    18541 agcggcatct gcacgatgct cccagatttc acgcagcgca cccagtggtg ctttttgctg
    18601 acgggccagc accagcgcag cgtaagactg aacggcgaat ttactggctt tgagattatc
    18661 cgcgtacggg atcgacatca tgcccggatc ttgtaaatag cgcagcagac gctcattacc
    18721 ccggttaatg gcgtctgtcg gcacgctgta accctgttcg cctgcgcgga ccaggaaatc
    18781 catcacgtaa gccgtcagcc agtactcttc gtcaccgttt ttatcccaca gcgcaaagcc
    18841 gccgttatca cgttgcattt gcagcaaacg ggaaatgccg atatcgaccg atgcacggcg
    18901 tttctcatca ctgtcgcctt tgatgcccaa cgcctgcagt tgggctgcgt tggtataaag
    18961 tgacgggaac aggccgctgg cggtttgctc aagacagccg tacggatacg cttttaactc
    19021 tttgatataa cgtgcaatgt tcagcggtgg tttgccgctc aacaacaatt gcccttccag
    19081 cgtaacaggc gagaagtttt gcaatccatc cgccggaatc gcccatgtct caccaggctg
    19141 taacgccgta ccgtaattaa ccgtttgtgc cgggaacgcc ggacggacgc cgattttcca
    19201 ctgcttatgc tgatcggcaa cggtttcacc cggtaacgct aacccgctaa tggtggcctg
    19261 aatttctcca tcgccataac ccggcaatgc tcgcaccggg ataaacagcg tagtacgcac
    19321 acctggcgct aattcaacgg ctgcgggtga atcgctgacc agttcaagca aaccactggc
    19381 ggtcagggca acgttcagtt tttgcggttt atcggtaaga ttagtgatat ccagcgtcag
    19441 acgcgaggta tcgccactcg ccataaagcg cggcatgttc agttcagcaa tcaccggtgc
    19501 ggcaactatc actttgcttt cgttgctacc gaaatcatct gccgtccagg cttgcgccat
    19561 gacgcgcaat tcaccgttaa aatcgccaat cggcagtgta accgagcctt cgccctgttc
    19621 gttgagcgtt accgacagcg cctgctgcgc gacaatattg acgtgattga ccggcggttt
    19681 accaccacgt ttcagctcat caccatcgcc accgaaacgc agagctgcca gacgcccctg
    19741 accttcaata acctgaccgt aaatatcgta aatgtctgcg ccatagcgtt tctgaccaaa
    19801 gaacgcctgc cacggatctg gcgtaacgta gtcagtaata ttcagcacac cactatcaac
    19861 ggcagacacc agcacattca cctgtttagg cttctcgcca tttttagtgc tggctttaat
    19921 tttcacggtt aatggttgat tgggacgcat ttttgctggt gtttccagcg ccagatcgag
    19981 gcgacggttt tcatcaccaa gcggcagatg caacacacca accgcgcgtt ttggcgtcgc
    20041 ggagcgagat ttatcgccag gacgcaccac cagcgtactc aaataaagat catgacgatt
    20101 ccaggtttta tcgaccggaa tcgtcagatc cagcccttga gccggaacat caatctcttg
    20161 ccaccacagc ggcccttcac tggactcgac catcgcataa cctttacccg ccgttggcgc
    20221 ggcaatatgc aacttaatgg tatcgccggg gcgataactg gctttatcta gtttcagcgt
    20281 gacacggtcg ggtcgcactg cgccgctccc gtcgctgttg tcctgccagc tatagccagc
    20341 ccaaaaacgc acgctactga ccgcttcatt cggcgctttg acttccagac gataagcgcc
    20401 ccactctacc gggaaactga ccttaccggt ttcatccgcc tgaagatcca gagtttgttc
    20461 attttcgatc agatcttttt gatcaaactg tgactgccag ccttcatctt ctgaccagtt
    20521 ccagtagtaa tcgcggcgtt cgcgaatcag gcgcacctgc aagcccgaca cggctttttt
    20581 cacgccttgc gcatcgctat aaacgatgtc aaaagcggcg ttactgcctt catcaacaat
    20641 gggctgtttc accgtgctgt cagtgcgata atcgtaaacc gatttcaagg cgaactgcgg
    20701 acggagcccc ggcaatgcat cggcaggcca gatagcctgc tcagcgcggc gcgtcaccgg
    20761 gcgaccgccc gattccagca aactgccctg gaaaataacc tgcaatgggg aatgcgtttc
    20821 cttccactgg ctttctgtag aaacttcgcc gcgcccttta tcatccagcg tcaactgaac
    20881 ttcatccagc gtgcgggaaa ggttttcggc agctatatcg ccgaattcaa aaccaggtaa
    20941 ggctgacaca gcctcacgca gtgggcgcag gaaaagttgc ccttgcaaag tattaccatt
    21001 agcaggcgca ccgtacaggt agtatcccac cacggagaat ttcacttcat cattcggcgt
    21061 aagcggggtt ttctcaccgg tcagattcag cgccatacgc tctggcataa aatcttcgac
    21121 gtggaaatcc cacatccggt actggttatc gcccgtgttg gcgcgaatat gccacatacc
    21181 ggttgccgca ttgctatcga gtggccaggt aaagtggtag aggccattct ccggctgact
    21241 aacgacgttc ctgagtacct gcccgtcagg tttaatcacg tctaacttga tgggttgatc
    21301 gggcaatgct ttaccgtctg catcgcgcag taaaccattg aggattaccg tttcacctgg
    21361 gcgataaaga tcgcgtgggc caaacatgaa aaactgtttg ctatagcctg gcgcgccagc
    21421 aatgttaaat tctgctaagt ccagcgccgg aagttttaaa tcgagtagcg ttgtctgacc
    21481 gtctttacgt gccagcagta atgccgcatt tttatcattt tccagctgca catgcccctg
    21541 agcgtcactg gttgcctgag tcagagtctg tcctttctca tttaataaag agacttcaat
    21601 tccttgctgg gccgcgccgt tttccagact ttgggtaaag atatccagac gattgtgata
    21661 acggtgagct gaaacgccga tatcacttaa cgtaaacagc gtcgcgggat tactgtaatc
    21721 gtaacgtcca gcctgattca tcacagccag atacacgccc gcctgctgaa gcggtttgat
    21781 atcgcccagc ggcagcaata atttttcacg ggtgttacgc gcaggattga gatcaaaccg
    21841 tccggtgtag accagatccg ccatctgcag cagtttgtct gactgccagt tcgccagcga
    21901 attgcggtat tcccattggc taatgaatgc tggcagagat tctggcttaa cgcggaagaa
    21961 gttaacatcg acattattaa cgttgagcgc cattaccggc agcccttcaa cgactttgcc
    22021 aggcagcagc gaaccacggc tggcaaaacc gacgctgggt tggatgtcgc gggtagttat
    22081 agttttttcg taatctttac tgaaggttgc gttgttgagt gctttgacct ccttgccaat
    22141 agtaacgatc aaatcacgtt tcggttcgag gtggcgtaaa cgtagctctt taagattatc
    22201 tgacagctcc caggcaccat ccactttgcc gcttttttta tcgacgacat gaataacgcg
    22261 tgagaaatcc tgatccgggt cgagagggat ggagaacgtc agcaccagcg tggctgcacc
    22321 atcaagttgg acttcagaga gatccagcaa tgtcagcacc ttaccggcac tctgttgggc
    22381 cagtttttgt ctctccggca cggagagttt tgctgaactc gcgttttctg aagaggccgc
    22441 tttagtaact tcagaaggcg catctttttt caccgctgtt ggcgcgttat cgttgttgtc
    22501 gcaccctgcc agcgccagca ttagcatgca ggcggctacg cgtaactttt tcatttttca
    22561 tccctgccac aatggcccgt tagcaacgtc gaataattat tatgcgtgag aatttacgtt
    22621 atgtaattca gtatcacgcc catacaatcc gaattttagt gagaattgtg gctcagacag
    22681 ccaaatcagc ctctttcgct tgtcgctttt cactaaacat ccgacaattt agcctaaccc
    22741 cggcaaaaat ggagatgcct atgtccacga catggtttgt aggagccgac tggctcgccg
    22801 aacatattga tgacccggaa attcagatta tcgatgcccg catggcgtcg cctggacagg
    22861 aagatcgtaa cgttgctcag gagtatctga atggacatat tcccggcgca gtgttttttg
    22921 atatcgaagc gctttctgat cacacttccc cgcttccgca catgctgccg cgcccggaaa
    22981 cgttcgccgt ggcgatgcgt gaattaggcg ttaaccagga taagcacctg attgtctatg
    23041 acgaaggtaa tcttttctca gccccacgag catggtggat gctgcgcacc tttggtgtag
    23101 agaaagtgtc gattctgggg ggtggacttg caggctggca gcgcgatgat ctgctgttag
    23161 aagaaggtgc agtagagctg ccggaaggag agtttaacgc cgcgtttaat cctgaagccg
    23221 tggtgaaagt aaccgatgta ttattggcaa gccatgaaaa tacggcgcaa attattgatg
    23281 cccgcccggc tgcacgtttt aacgcagaag ttgatgaacc tcgcccaggt ttacgtcgcg
    23341 gacatattcc cggagcactg aatgttccgt ggacggaact ggtgcgcgaa ggcgaactaa
    23401 aaacgaccga tgaactggat gcgatatttt ttggtcgcgg cgtcagctac gacaaaccaa
    23461 ttatcgtcag ctgcggctct ggtgtaacgg cagccgtggt tttgttagca ctcgcgacgc
    23521 tggatgtgcc aaacgtgaaa ctgtacgacg gcgcatggag tgaatggggc gcgcgggcag
    23581 atttaccggt tgagccagtg aaataagtat tttacaggca ataaaaaacc gccgaatttg
    23641 gcggtttttt attgctggtc tggttcgcgg cctttccagc aggttgtatt accgtagtaa
    23701 tgcaagcgcg tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag
    23761 agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag
    23821 cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct
    23881 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
    23941 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt
    24001 aaataataac cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag
    24061 caagtaagcc cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta
    24121 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcacaatatg ttcggtaata
    24181 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
    24241 ggtaatgtat cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg
    24301 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
    24361 aaagcacgct tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
    24421 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
    24481 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
    24541 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
    24601 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct
    24661 aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
    24721 agcgcactat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
    24781 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
    24841 agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
    24901 ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg
    24961 aatttgcaga atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac
    25021 gccgttaaca gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt
    25081 tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag
    25141 ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg
    25201 cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc
    25261 agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagcaa acgaccggca
    25321 agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc
    25381 ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga
    25441 ccatcggcaa gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg
    25501 ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca
    25561 cagcagagga acagcttcac gcgcttgttc agtccgcgcg taatggcaaa tgccagcgcc
    25621 ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt
    25681 ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacact
    25741 ggcgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct
    25801 gaaccgcgtc cgacggtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg
    25861 gtgatccgat aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt
    25921 gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg
    25981 atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc
    26041 actttacgcg tgcctttcgg ggctttgtaa ccttgccaga atgcccagca gcgatccgca
    26101 tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgcgcc
    26161 gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta
    26221 ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta
    26281 atcttcatcg cttctgtcat tttagttatc cttctttgta aaagggccgc cagttggcag
    26341 ccccaggatt tttccgctaa atgattcgcg ttgcagaaag gcggcaagtg agtgaagccc
    26401 caggagcata gataactatg tgactggggt gaacgagcgc agccaacgca tctgcggcgt
    26461 gaagcatgac gcggaaatta ttcggcctcg tccagccaga ctaacaaaat cgcttcgagg
    26521 attttctcgt tggatgcctg cgggtcgtcg tcgaaatctt ccagatcgca aatccactga
    26581 tgcatatcgg tgaatcgaac cgttttcgga tcaagatcag gatacgcatc gtacagtgct
    26641 tcgccaattt cgcggctatc ggtccactta agtcccatac taacctctgt taatgctcac
    26701 gcgcatggtt gatagtgtaa cgcgggattt cgactactaa atcttcgtcg gtaacacgtg
    26761 cctggcagct taaacggctt tcgggctcca gtccccaggc tttgtccagc atgtcgtctt
    26821 cctgctctga gctttctggc agcgagtcaa aaccttcacg aacgatgcag tggcaggtgg
    26881 tgcaagcaca ggatttttca caggcgtgtt caatctcgat accgttacgc agagctgcgt
    26941 cgagaatggt ttcaccgcta ttagcttcca gaacagcgcc atcaggacag agatcctgat
    27001 gaggcaaaat aacaatcttt ggcatattaa acctcgtcca cggaatggcc tttcagcgca
    27061 cgacgaaccg actggtccat gcggcgagcg gcgaaatcct gggtttgttt gtctacgttt
    27121 ttaatcgctt gttcgatggc gtcaacgtca tcgccctgcg ccacttcact caggtgagcg
    27181 gcagcatcgt caatgacctg acgttctgcg gcgcttaaca gcgcggcatc agcagccagc
    27241 gcgccgtgca gactttccag cacacgcgcc gcttctactt tttgttctgc cagcattcgg
    27301 gcttttacgt cctgctcggc atagctcatt gagtctttga tcatcgaagc gatttcgcta
    27361 tcggtcagac cgtaagacgg tttgacctga atagacgcct caacgccggt ggatttctcc
    27421 atcgccgtca cgctcaaaag accgtcggca tcgacctgga acgtcacgcg aatatgcgca
    27481 ccaccagccg gtagcgccgg aataccacgc agcgcaaaac gcgccagtga gcggcagtcc
    27541 tgcaccagtt cgcgctcacc ctgcattaca tggatagaca tcgccgtctg accatcttta
    27601 aaggtggtga aatcctgagc gcgggccacc ggaatagtgg tattacgcgg aatcactttc
    27661 tccaccaggc cgcccatcgt ttcgaggccc agcgacagtg ggatcacatc aagcagcagc
    27721 atttcgctgt ctggcttgtt acccaccaga atatccgcct gaatcgccgc gccaatagcg
    27781 acgactttat ccgggtcgat ggaagtcagc ggtggacgac cgaaaaattc gcctacccgt
    27841 tcacgcacca gcggcacgcg agtagaaccg cccaccatca ccacttccag cacttcgtca
    27901 gcttctacac ccgcgtcttt aagcgcgcga cgacaagcca gtaaggttcg ttttaccagt
    27961 ggcgcgatca gttcattgaa ttgttcacgg ctgatttcgc cctgccagcc cgcaacgtta
    28021 acggtcacgg agtccgcatc gctcagcgcg attttggctg caatggcggc atccagcagt
    28081 tcacgctgaa cgcggttatc gctacgatca ggaatgcccg cctgctcgcg aatgtaatcc
    28141 gccagcagat ggtcgaaatc atcgccgccg agcgcggaat caccgccggt tgccagcact
    28201 tcaaacacgc cgcgacttaa gcgcagaatg gaaatatcaa acgtcccgcc accgaggtca
    28261 taaacagcga tcacgccttc ctgaccggaa tccagcccgt aggcgatagc cgcagcggtc
    28321 ggttcgttaa gtaagcgcag aacatgcaac cccgccagac gcgccgcgtc tttggtgccc
    28381 tgacgctggg catcgtcaaa gtacgccgga acggtgataa ctacaccatc cagctcgcct
    28441 gccagggctt cagttgcccg cgccgccagt gctttgagga tgtccgcaga aacgcgcacc
    28501 gggttcagca gccccgccgc cgtttcaatc atcggcaggc cgttttcgct ggcctggaat
    28561 tgataaggca gatgcggata gcgttgctgg atatcagcca gcgagcgccc catcaggcgt
    28621 ttaacagaac taatggtgtt ggcggtgtca agcgctgcgt tagtacgcgc gtcataaccc
    28681 accgaatgcc cttgctgttg atagtgaaca acagatggca gcaggtgacg gccttcatgg
    28741 tcggctaacg tttcggcctg accgctgcgc actgtcgcca ccagcgagtt ggttgtgccc
    28801 aggtcaatac cagccgccag acgacgctga tgcggcgcag cactcaaacc aggttcacta
    28861 atttgtaata aggccatgtt tagcttccag aaattaaaaa tcgagcagtt tttcttcgag
    28921 ttgttcggca ctgctccgca gtttatcgag aaaacgcagc ttacgcacgg tatccgccgc
    28981 cgcgtcccac gcctcgttgt ctaactgttc aaccatcaac tgatggcggg tatcaaacat
    29041 ctttttcaca cgtttgataa agctttccag ccgcgcttca tctttcgcct gttcgatctc
    29101 gtccagctct tcgcgcagct ccaactgttc catcaggaac gcggtgtcgc gcacagtatg
    29161 ctgctcgctg gcgagatcaa aaccgtgtaa agaaagcaaa tattccgcgc gcattaacgg
    29221 atgacgcagc gtttgccagg cctggttaat ggttgcagat tgctgtacgg cggcgagttg
    29281 ttccgcctgg cttccgctgg cgaatttatc aggatgatac tgacgttgta gatcctgaaa
    29341 acgcaggctc agcgcctggg tatcgagttg ataacgggca ggcaagccaa agaaggtgaa
    29401 gtaatccata acattctcag ggctgcgatc cgccgcgata acggcgaatc aggtaaaaca
    29461 aaccccacgc gcaggcgacc acggtggggt tatcggtatg cgcatcaaac gtggaagctt
    29521 tcgccgcaac cacactcatc tttgacgttc gggttggtga atttaaaccc ttcgttcagg
    29581 ccttctttta cgaagtccag ctgcgtaccg tccagaaatt gcaggctttt gccatcgacc
    29641 acgactttca cgcctttgtc ttcaaacacg atgtcttccg gcgtcggttc gtcaacaaat
    29701 tccagtacat aagccatacc tgaacatccg gaggttctca cgcccagacg caggccaaac
    29761 cctttgccgc ggttagccag aaaggtattt actcgcgctg ctgcactgtc gctcagtgta
    29821 atcgacataa ccaaacctca actcttattt tgcttcacgt ttgcttttat agtccgcaat
    29881 ggcggctttg atcgcgtctt ctgccagaat agaacagtga attttcaccg gcggcagttc
    29941 aagttcttca gcaatatcgg tatttttgat cgcctgcgct tcgtcgagag acttcccttt
    30001 cacccattcg gtgaccaggg agctggaagc gatagcagaa ccgcagccgt aagttttaaa
    30061 acgcgcgtct tcaatgatac cttcatcgtt gactttaatc tgcaacttca tcacgtcgcc
    30121 acaggccggt gcccccacca tgccgctgcc gacgttctcg tcgttgttgt caaaggaacc
    30181 cacgttacgc ggattctcgt aatggtcgat aactttttcg ctgtaagcca ttataaattc
    30241 tcctgattcc gataccgatt aatgatgagc ccattcgatg ctgttcagat ccacgccctg
    30301 cttgtacatt tcccacagcg gagaaaggtc acgcagacga ccgatggatt tacgaactaa
    30361 ctcgatggtg tagtcgatct cttcttcagt agtaaaacga cctaaagaga aacggataga
    30421 gctatgtgcc agctcgtcgt tcagccccag cgcgcgcagc acgtaggacg gttcgaggct
    30481 tgctgacgta caggcggaac ctgaagaaac tgcgaggtct ttcagcgcca taatcagcga
    30541 ctcaccttca acgtagttga agctgacgtt gagaatgttc ggcgcaccgt gttccaggtc
    30601 accgttcagg taaacttctt cgatatcttt gatgccgttc cacagacggt tacgcaggcc
    30661 gcgcagacgt tccatctcgg tcgccatctc ttcttttgcg atgcgatagg cctcgcccat
    30721 tccgacgatc tggtgaacag gcagagtgcc ggaacgcata ccgcgctcgt gaccgccgcc
    30781 gtgcatttgc gcttcgatgc gtacgcgcgg tttacgacgt acatacagcg caccgatacc
    30841 tttcgggcca tagattttgt gaccggagaa agacatcagg tcaactttca actggctcag
    30901 gtcgataggc agtttgccca cgctctgggt tgcatcaacg tgatagataa tgccacgagc
    30961 acggcacatt tcgccgatag ccgcgatatc ctgcaccacg ccgatttcgt tatttacgtg
    31021 catgatggaa acaaggatgg tgtcatcacg catcgctgct tcaagttctt tcagatcgat
    31081 aatgccgtta cgctgcggtg ccaggtaggt gacttcaaaa ccttcgcgct ccagctgacg
    31141 gcaggtatcc agtaccgctt tgtgttcggt tttgctggtg atgatgtgct tgcctttttt
    31201 ctgataaaag ttggctgcac ctttgatcgc caggttgtca gattcggttg caccagaggt
    31261 aaagacgatt tcacgcggat cagcgccgac cagatcggca atctgattac gggcgatatc
    31321 taccgcttct tcagcctgcc agccgaaacg gtgagaacgg gaggccgggt taccaaaggt
    31381 tccgtccatc gtcataaact gcatcatttt ctcggcaaca cgcgggtcca ccggcgtggt
    31441 tgcggagtag tcgagataaa tcggtaattt cattgctcta taaactccgt acatcactca
    31501 atgcaaggaa ccaggctacc ggctggatgt acgaccgtgt ttacggagcg cgatacgccc
    31561 cggcctgatt ctaatatctt gttatcttta acctaactgt ttcgggtttc agcaagttga
    31621 caaacttacg aatctccggg agcttacata agtaagtgac cggagtgagt atgtgcagtc
    31681 aacgcagatg caacacgaaa caggacggtt aaattaagcg cgtaacttaa cgtcgatcgc
    31741 gtcttgtatg cgggtgcgtg gcgcgtcgtg agtatgctga cgaccagaca catccagcac
    31801 ttcctggtta ttaaccagtt cgcctaaagt aatgttgttg agaaaaccgg tgagacggtc
    31861 gctcaaatca cgccacagcg cgtgggtcag gcatttatcg ccgccctggc aaccgccttt
    31921 accctgacaa cgggtagcat ctacagattc gtcaacggcg ctaatcactt cgccaacggc
    31981 gatgctgctg gcatctttgc ctaacagata accaccgcct ggtccacgta cgctggaaac
    32041 cagaccattt ttacgcagac gggaaaacag ttgttccaga taagaaaggg aaattccctg
    32101 acgttcggaa atatcagcca acggtaccgg gcccgcttca gagttgagcg caacgtcaag
    32161 cattgcggtc acggcatagc gccctttaga tgtcagtctc atgtcttact tcacctcaaa
    32221 ctcgcccctg cccggggttt tttattgtaa aagtgggggt attgcatagc agggtcaagt
    32281 ctgacattcc cgagtaaatt ggtcaactat ttacttgact gatttagtcg ggtatttaac
    32341 cttcagtgcc atttttttat cgtggcgttt gtacctgttg tccttccaga ccatgggcgg
    32401 gttgagggca attattcgca ttagcgctgg cctcttcccc tcaccctaac cctctccccg
    32461 aaggggcgag gggaccgtcc gtgcatttat ctggcttagc cctcaggcct tattaccttt
    32521 attctgctgc tctatagaag ccagaatccc gcgcaggata ttcaattcct ggctttccgg
    32581 gcgcgcacgg gtaaacagac ggcgcaattt attcatcacc tgccccggat ggttttcacg
    32641 gataaaaccg gttgccagca gcgtttgttc cagatgacca taaaaacgct ccagatcatc
    32701 gaccagcgga tacggcgtct cttcatgctc gacctgctcg ccattttcct gagttgccag
    32761 ccaggccata cgcacttcat aggcaataac ctgaaccgcc attgccaagt tcagcgaact
    32821 gtattccggg tttgccgcaa tcgcgacatg ataatggcat ttctgcaact cttcattggt
    32881 caagccgacg cgctcacgac caaacaccag cgccaccggg gtatttgccg cttcagcgac
    32941 gcttttcaga ccgcattcgc gcgggtcgag catcggccac ggcagcgtgc gggagcgtgc
    33001 gctggtgccc accaccaggc tacaaccggc taacgcttcg tcgagcgtat cgacgatgtg
    33061 agcattacca atcacatcgc tagcccctgc tgccagggca atcgcctggg agtcgggttt
    33121 caccagtgga ttaaccagcc acagattggt taatcccatt gttttcatgg cacgggcaac
    33181 agaacccata ttgccggtgt gtgacgtctc caccagcaca attcgaatat tttgcagcat
    33241 tgtctttctt catctaaaga ttattcacgc atcttatcat aaaacgaaga cagatgccga
    33301 tctcgctgct atactctgcg ccgttttccc gttctttaac atccagtgag agagaccgat
    33361 gcatccgatg ctgaacatcg ccgtgcgcgc agcgcgcaag gcgggtaatt taattgccaa
    33421 aaactatgaa accccggacg ctgtagaagc gagccagaaa ggcagtaacg atttcgtgac
    33481 caacgtagat aaagctgccg aagcggtgat tatcgacacg attcgtaaat cttactcaca
    33541 gcacaccatc atcaccgaag aaagcggtga acttgaaggt actgatcagg atgttcaatg
    33601 ggttatcgat ccactggatg gcactaccaa ctttatcaaa cgtctgccgc acttcgcggt
    33661 atctatcgcc gttcgtatca aaggccgcac cgaagttgct gtggtatacg atcctatgcg
    33721 taacgaactg ttcaccgcca ctcgcggtca gggcgcacag ctgaacggct accgtctgcg
    33781 cggtagcacc gctcgcgatc tcgacggtac cattctggcg accggcttcc cgttcaaagc
    33841 aaaacagtac gccactacct acatcaacat cgtcggcaaa ctgttcaacg aatgtgcaga
    33901 cttccgtcgt accggttctg cggcgctgga tctggcttac gtcgctgcgg gtcgtgttga
    33961 cggtttcttt gaaatcggtc tgcgtccgtg ggatttcgcg gcaggcgagc tgctggttcg
    34021 tgaagcaggc ggcatcgtca gcgacttcac cggtggtcat aactacatgc tgaccggtaa
    34081 catcgttgct ggtaacccgc gcgttgtgaa agccatgctg gcgaacatgc gtgacgagtt
    34141 aagcgacgct ctgaagcgtt aatgactcag gcgggtgatg tcactcaccc gccctcgcct
    34201 ttcaggcgct attctgaaat acttcctcac cgctttactt tctttcgtca ctctcccacc
    34261 atctttcccc gattaatgga tgataaagaa gtcacaagga aaggttatgg cgctgccagt
    34321 gaacaaacgc gttctcaaaa ttctgtttat tctctttgtt gttgccttct gcgtttattt
    34381 agtgccgcgc gttgccatca acttcttcta ttatcctgac gataaaattt acggtcccga
    34441 cccctggtcg gcggaatccg tcgaatttac ggctaaggac ggtactcgtc tgcaaggctg
    34501 gtttattcct tcttcgacgg gccctgctga caatgccatc gcaaccatca ttcatgctca
    34561 cggcaatgcc ggaaatatgt ccgcccactg gccgctggtc agttggttac ccgagcgtaa
    34621 tttcaacgtt tttatgtttg attatcgcgg gtttggtaaa tcaaaaggca cgccgtccca
    34681 ggccggattg ctggacgata cgcaaagtgc catcaatgtg gtgcgccatc gcagtgatgt
    34741 aaacccacaa cggctggtgc tgttcgggca gagtattggc ggggcgaata ttcttgctgt
    34801 tattggtcag gatgatcgtg aaggcatacg tgcggtgatc ctcgactcca catttgcctc
    34861 ttatgcaacc atcgccaatc agatgatccc cggcagtggc tacttacttg atgagagtta
    34921 cagcggcgaa aattatatcg ccagcgtcag cccgatcccg cttttactca ttcacggtaa
    34981 agctgatcac gttatcccat ggcagcacag cgaaaagttg tatagcctgg caaaagagac
    35041 aaaacggttg atcctaatcc cggatggcga acacattgat gctttttccg atcgtcacgg
    35101 cgatgtttat cgcgaacaga tggtggactt tatccttagc gcgttgaatc cgcagaacta
    35161 acccatgatc gctagcacga taatcattca caaaaccacc ttaagacatg ctaatccact
    35221 ggtcagaaca gtttaagatg agaaaaattc tgtgacgctt gccaacattt ctgatgatta
    35281 gcattccctt cgccatttcc ctgagcaaac tttagctatt cttatcaatt atgcttatgg
    35341 gagatctaca gatgatgcct acgcttgctc caccatctgt cctttcggct ccccagcgcc
    35401 gctgccagat cttgctgacg ctctttcagc cggggttgac cgccaccatg gcaaccttca
    35461 gcgagcttaa tggtgtggat gatgatattg ccagtcttga tatcagcgaa acaggacggg
    35521 agatcctgcg ctatcatcaa ctcacactga cgactggtta tgacggtagc taccgggttg
    35581 aaggtacagt gcttaaccaa cgtttgtgtt tatttcactg gctacgacgt ggtttccgtc
    35641 tgtgtccgtc atttattacc agccaattca cccccgccct gaagagtgaa ctgaagcggc
    35701 gcggaattgc gcgtaacttt tacgacgata ccaatctaca agcgttagtg aatctctgct
    35761 cccgacggct gcaaaaacgc tttgaaacgc gcgatattca tttcctgtgt ctgtatctgc
    35821 aatattgttt gctgcaacac cacgctggaa ttacgcctca gtttaatccg ctccaacgtc
    35881 gctgggcgga atcctgcctt gaatttcagg tagcgcagga aattggacgc cactggcagc
    35941 gtcgggcgct ccagcctgta ccgcctgatg agccactgtt tatggcatta cttttttcca
    36001 tgttgcgggt tcccgatcca ttgcgtgatg cgcatcagcg agacagacaa ttgcgtcagt
    36061 ctatcaaacg tctggtaaac cattttcgtg agctgggaaa tgttcgtttt tatgatgaac
    36121 aggggttatg cgatcagctt tatacccacc tcgcccaggc gttaaatcgc agtttgtttg
    36181 ccatcggtat tgataatacc ctgccggaag agttcgccag actgtaccca cgcctggtgc
    36241 gcaccacccg cgcggcgctg gccggatttg aaagtgaata cggcgtccat ctttctgatg
    36301 aggaaagcgg tctggtcgcg gtgattttcg gtgcctggct aatgcaggaa aacgatctgc
    36361 atgaaaaaca gattattcta ctgaccggga atgatagcga gcgagaagcg cagattgagc
    36421 agcagttacg cgaactaacg ttactgccgc tcaacattaa gcatatgtcg gtaaaggcat
    36481 ttttgcagac aggcgctccg cgtggcgcgg cactgattat tgcgccttat accatgccat
    36541 taccgctctt ttcaccaccg ctgatctata cggacctgac gctgacaaca catcaacagg
    36601 agcagatccg caaaatgctc gaatcagcat gagggaacaa ctttcgggcg caaaaacatt
    36661 gccggaagcg ccaccagcgc cattacccag aacacgccgt ggcccagata ttgatacagg
    36721 aaaccggcga aaacggtcat gatagcgata ctgccgccca tcgcgacggc agagtaaacc
    36781 gcctgtaaac ggatgacttc gctaccctgg cgaacagcaa tatagcgcat ggcggccagg
    36841 tggcagaccg tgaaggtgcc gcaatgcaaa atttgcacca ctatcaacca cggcaacgcc
    36901 gtagttgctc ccataatgcc ccagcgcact acgccgcaaa tcgccgagat caacagcata
    36961 tcgcgagcac tacaacggcg gaaaagttta ttactcagcg caaagataat gacttccgcc
    37021 accacgccca gcgaccacaa ataccccacc gccgaggccg agtagccagc tgcctgccag
    37081 taaatggcgc taaaaccgta ataggccgca tgtgccccct gcaataaaca aacgcaggcc
    37141 agaaagcgcc agttctggcg aaccagcgcc aaccacgcag accaaccggt gctctcctgc
    37201 tggcgacttg ccccttgtgg ctgaatcgtc ggacggatga gaaagccgag cagcatggat
    37261 gccacgccca acgtcaacag cgcgaggatc acccgataat caaacatagt gaccagtttg
    37321 cccgtcagcg ccgagccaat gacaaacgcc accgagcccc acagtcgcac tttgccgtaa
    37381 tcaagcggga actgcttttg ccacgtattc gccagtgcat cggtcaacgg taccagcggt
    37441 gagaaaaaga ggttaaagcc aatcatcacc agcatcagcc acgctacgtg cgcccccgcc
    37501 cagaaggcga cagcaaagag aagtgtcagc agtgccagca cgcgcaaggc ggaaatcagg
    37561 cgggaaggat cgctgacgcg gggcgcgatg agcaaactcc cgagaaaacg ggcaaccaga
    37621 cctgccccca ataacaggcc gatggtttct ggcgttaaac caatcccttt aagccagacg
    37681 ctccagaaag gtagaaaaat gccgtaacta aaaaagtatg tgaaatagcc gagcgccaac
    37741 cagcgcgtgg attgcaaaac catgagtccc tcccgtcagg aggcgttagt ctggggttaa
    37801 tcgaatgttg gtgcaagttg aaaaataaga atattcgagc ctgcttcccg atgtctgtaa
    37861 cgccagttca caagcactaa aaaactgcga tccgcgacgc atgttgcaaa aataatgcgt
    37921 aattatgccg ttacgcttgc caaacgttcc tgcacaatgg cgatgaaatc gcgtaatgcc
    37981 ggcttcatct cccctttctt ccacgccatc agtaaagcaa tggaaggcac atttccggca
    38041 atgggacgaa aaacaacctg cccggtatta aaattattca tataaccggg tatcaaagtg
    38101 acgcccagcc ccatgcccac cagattcatg gtcaccagaa tattcgttgc cacctggacg
    38161 atatttggct ggctgttttc ttgcgcaaac cacgctttaa cgatcggcgc aagcgaaccg
    38221 gaatacgccg gatcggtact gacgaaattc acgccatcca gttacgcggc ggtgatctct
    38281 ttttcatgtg ctaaagggtg atcaaccggt aacaccacca ctaatggttc gtcaaaaagc
    38341 tccagataat caatctccgg gctataaaca ggatggcgca ttaagccgac gtcgagttca
    38401 ccacggcgaa ttttttcctc ctgttgcgtg gtgattaaac tcaccagctc aatcaaggtg
    38461 tctggctgtc tgagacgaaa cattggtaat acttttggca ataaattcac ttccgccgat
    38521 ggcacaaagc caatggttaa ttgtctgtct tcctgaacaa ttttccgcgc ccgtaatttg
    38581 gcattttccg cttgttcgag gattgccagc gcgtcctgga gaaaacattc tcccgccgcc
    38641 gtcaacgcga ctttgcgctt atccctcacc agtaacggaa caccgacaca gttttcaaga
    38701 tcgcggatct ggctgcttaa cgaaggctgt gaggtatgca gtttttccgc cgcacgggta
    38761 aagttcagtg cctgcgccac tgcgacgaaa tagcgtaaat gccgtagttc catcaccttc
    38821 cccttgttat cgaaaaaacg tctcagctgg tagaaattaa ctatttcaca tattagcaac
    38881 caaccaacaa catccttatg acacaaaaat agaaggtcaa tacatcttat ctttcaggat
    38941 taaaaaatat gaccacaccc tcagatttaa acatttacca actgattgat acccaaaatg
    39001 gtcgggtcac tccgcgtatt tataccgacc cagatattta ccaactggag cttgaacgta
    39061 ttttcggccg ttgctggtta tttctcgccc acgaaagcca gatcccaaaa cctggtgatt
    39121 tctttaacac ctacatgggg gaagatgcgg ttgtcgtagt gcgtcagaaa gacggcagca
    39181 ttaaggcgtt tctcaaccaa tgccgccacc gggccatgcg tgtgagttat gcagattgcg
    39241 gcaacactcg cgcctttacc tgtccgtatc acggctggtc ttatggcatt aacggcgagt
    39301 tgatcgatgt accgctggaa cctcgcgcct acccacaagg gttgtgtaaa tcccactggg
    39361 gactaaacga agttccttgt gtggagagtt ataaagggct gatttttggc aactgggata
    39421 ccagcgcacc gggcctgcgt gattacctgg gtgacattgc ctggtatctg gatggcatgc
    39481 tggatcgtcg cgaaggcggc accgaaattg tcggcggcgt acaaaagtgg gtgatcaact
    39541 gtaactggaa attcccggca gagcagttcg ccagtgacca gtatcatgct ctgttcagcc
    39601 atgcttctgc cgttcaggta ttaggggcga aagatgatgg cagcgataag cgcctcggtg
    39661 atggacaaac cgcccgcccg gtgtgggaaa ccgccaaaga tgcgctgcaa tttggtcagg
    39721 acggtcacgg tagcggtttc ttctttactg aaaaaccgga tgctaatgtc tgggtcgatg
    39781 gcgcagtttc aagctattac cgcgaaacct atgccgaagc agaacaacgt ttaggtgaag
    39841 ttcgcgccct gcgcctggcg ggtcataaca atattttccc cacgctttca tggctcaacg
    39901 gcactgccac gctccgcgtc tggcatccgc gcggccctga tcaagttgaa gtgtgggcgt
    39961 tctgtattac tgacaaagcc gcctccgatg aagttaaagc cgcttttgaa aacagcgcca
    40021 ctcgtgcttt tggtcctgct ggttttctcg agcaggatga ctcggagaac tggtgtgaaa
    40081 tccagaaatt gcttaaaggc caccgcgccc gcaacagcaa actgtgtctg gaaatggggc
    40141 ttggtcagga aaagcgccgc gacgacggca ttcctggcat tactaactat atcttttcag
    40201 aaactgccgc tcgtggaatg taccaacgct gggccgatct tctgagtagc gaaagctggc
    40261 aggaagtgct cgataaaacc gccgcttacc agcaggaggt gatgaaatga gtgcgcaagt
    40321 ttcactagag ttacatcacc gcattagcca gtttctcttt cacgaagcca gcttactgga
    40381 cgactggaaa tttcgtgact ggctggagca gctcgacaaa gagattcgtt acaccatgcg
    40441 caccacagtt aacgcgcaaa cacgcgaccg ccgcaaaggc gtccagccac cgacaacctg
    40501 gatttttaat gacaccaaag accagctgga gcggcgaatc gcccgtctgg aaacgggcat
    40561 ggcctgggca gaagagccgc cgtcacgcac ccgtcactta atcagcaact gccagataag
    40621 cgaaaccgac atcccaaacg tatttgctgt gcgggtaaat tatctgcttt atcgggcaca
    40681 aaaagagcgc gatgaaacat tctatgttgg aacgcgtttc gacaaagttc gccgtctgga
    40741 agatgacaac tggcgcttgc tggaacggga tatcgtcctg gatcaagcgg taatcacttc
    40801 ccataacctg agtgtactgt tctgatgaat cgaatttatg cgtgtcccgt tgcggatgtg
    40861 ccggagggtg aggctctccg gatcgatacc tcgcccgtca tcgccctgtt caacgttggc
    40921 ggcgagtttt atgccattaa cgatcgttgc agccatggta atgcgtcgat gtcagaaggg
    40981 tatctggaag atgacgccac ggtggagtgc ccgctacacg ccgccagttt ttgcctgaaa
    41041 acggggaaag cgttatgcct gcccgccacc gatccgctca ccacttatcc agtacacgtt
    41101 gaaggtggtg acattttcat cgacttaccg gaggcgcagc catgagcgat ctgcataacg
    41161 agtccatttt tattaccggc ggcggatcgg gattagggct ggcgctggtc gagcgattta
    41221 tcgaagaagg cgcgcaagtt gccacgctgg aactgtcggc ggcaaaagtc gccagtctgc
    41281 gtcagcgatt tggcgaacat attctggcgg tggaaggtaa cgtgacctgt tatgccgatt
    41341 atcaacgcgc ggtcgatcag atcctgactc gttccggcaa gctggattgt tttatcggca
    41401 atgcaggcat ctgggatcac aatgcctcac tggttaatac tcccgcagag acgctcgaaa
    41461 ccggcttcca cgagctgttt aacgtcaacg tactcggtta cctgctgggc gcaaaagcct
    41521 gcgctccggc gttaatcgcc agtgaaggca gcatgatttt cacactgtca aatgccgcca
    41581 ggtatcctgg cggcggtggc ccgctgtaca ccgccagtaa acatgccgca accggactta
    41641 ttcgccaact ggcttatgaa ctggcaccga aagtgcgggt gaatggcgtc ggcccgtgtg
    41701 gtatggccag cgacctgcgc ggcccacagg cgctcgggca aagtgaaacc tcgataatgc
    41761 agtctctgac gccggagaaa attgccgcca ttttaccgct gcaatttttc ccgcaaccgg
    41821 cggattttac ggggccgtat gtgatgttgg catcgcggcg caataatcgc gcattaagcg
    41881 gtgtgatgat caacgctgat gcgggtttag cgattcgcgg cattcgccac gtagcggctg
    41941 ggctggatct ttaaggaagc acgatgaaag aaaaaacgat cattattgtc ggtggcgggc
    42001 aagcggcggc aatggctgcg gcctcgctac gccagcaagg gttcaccggt gagctgcatc
    42061 tgttttccga tgagcgacat cttccttatg aacgacctcc actctcgaaa tccatgttgc
    42121 tggaagattc cccgcagtta cagcaggtgt tacccgctaa ctggtggcag gaaaacaatg
    42181 ttcatctgca ttccggtgta accatcaaaa cgctgggtcg cgacacacga gagttagtgt
    42241 taaccaacgg cgaaagctgg cactgggatc agctttttat agcaatcggc gcggcagctc
    42301 gaccgctgcc gttgcttgat gcactgggag aacgctgctt taccctacgc catgccggtg
    42361 atgccgccag actgcgagaa gttctgcagc ccgaacggtc agtcgtgatt gtcggtgccg
    42421 gaactattgg tctggaactg gctgccagcg ccacgcagcg cagatgtaag gtgacagtga
    42481 ttgaactggc ggcaaccgtc atgggccgta atgcaccacc gcccgtgcaa cgctatcttt
    42541 tacagcgcca ccagcaggct ggtgtgcgca ttctgctcaa taatgccatt gaacatgtgg
    42601 tcgatggtga aaaagtagaa ctgacgctgc aaagtgggga aacgcttcag gctgatgtgg
    42661 tgatttacgg tattggtatc agcgccaacg agcaactggc tcgcgaggcc aaccttgata
    42721 ctgccaatgg cattgtcatt gatgaggctt gccgcacctg cgatcccgcg atctttgccg
    42781 gtggcgatgt ggcaatcact cgtcttgata atggtgcact acaccgctgc gaaagctggg
    42841 aaaacgccaa taaccaggcg caaattgccg ctgccgcaat gttagggcta ccgctaccgc
    42901 tactgccgcc gccgtggttc tggagcgatc agtacagtga taacttacag tttattggcg
    42961 atatgcgtgg cgatgactgg ctttgtcgtg gcaacccgga aactcagaag gcgatttggt
    43021 ttaatctgca aaacggcgtg cttatcggtg cggtcacgct gaatcagggg cgtgagattc
    43081 gcccaattcg caaatggatc cagagcggca aaacgtttga tgcgaaactg ctgatagatg
    43141 agaacatcgc gcttaaatca ctgtaaccag gataattagc gaatatctca acgcctgggg
    43201 cgtggcgagg tgcaagagtg tgtattacgt ttaaatcaca ttatcttgca aagggattgg
    43261 ttatgaacac actac
//
LOCUS       DAEEKQ010000015        42056 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.015, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000015 DAEEKQ010000000
VERSION     DAEEKQ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42056)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42056)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42056
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.015"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..776
                     /locus_tag="KLK57_000834"
     CDS             <1..776
                     /locus_tag="KLK57_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBE0891288.1"
                     /translation="NTRDMILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYI
                     LTLLNSMRNMDGNKDSRINKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTL
                     DVCHYDSETKVLTSHHGIIRNEENECYCLGALQVPFNQMKDPIDPEELANIFNKKHKQ
                     HMDDRLIHLIRSNAVASTPVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHYGLG
                     VDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPQ"
     gene            1038..2660
                     /locus_tag="KLK57_000835"
     CDS             1038..2660
                     /locus_tag="KLK57_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HBE0891289.1"
                     /translation="MKIGTVAGTNDSTTTITTNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWK
                     ISQYNVFDIKFETMIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNIIISILNRRDIVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDKFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYSGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSSDEMTNALDPVELAAIINKKHHAVINGKKFKNSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRECIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            3026..4093
                     /locus_tag="KLK57_000836"
     CDS             3026..4093
                     /locus_tag="KLK57_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0891290.1"
                     /translation="MDKSKRHLAWWVVGLLVVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQSELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELSWPDDLVVRLPQ"
     gene            4090..6825
                     /gene="rbbA"
                     /locus_tag="KLK57_000837"
     CDS             4090..6825
                     /gene="rbbA"
                     /locus_tag="KLK57_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HBE0891291.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKNVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPRHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRSQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            6825..7949
                     /locus_tag="KLK57_000838"
     CDS             6825..7949
                     /locus_tag="KLK57_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0891292.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMAKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            8022..8295
                     /locus_tag="KLK57_000839"
                     /pseudo
     CDS             8022..8295
                     /locus_tag="KLK57_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
     gene            8292..8651
                     /locus_tag="KLK57_000840"
     CDS             8292..8651
                     /locus_tag="KLK57_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HBE0891293.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(8771..9172)
                     /gene="nikR"
                     /locus_tag="KLK57_000841"
     CDS             complement(8771..9172)
                     /gene="nikR"
                     /locus_tag="KLK57_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HBE0891294.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(9178..9984)
                     /gene="nikE"
                     /locus_tag="KLK57_000842"
     CDS             complement(9178..9984)
                     /gene="nikE"
                     /locus_tag="KLK57_000842"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HBE0891295.1"
                     /translation="MTLLNVSGLSHHYAHGGFNGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPAQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(9981..10745)
                     /gene="nikD"
                     /locus_tag="KLK57_000843"
     CDS             complement(9981..10745)
                     /gene="nikD"
                     /locus_tag="KLK57_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HBE0891296.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKAVYPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHAVTRSLVSAHLALYGMELAS"
     gene            complement(10745..11578)
                     /gene="nikC"
                     /locus_tag="KLK57_000844"
     CDS             complement(10745..11578)
                     /gene="nikC"
                     /locus_tag="KLK57_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HBE0891297.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(11575..12519)
                     /gene="nikB"
                     /locus_tag="KLK57_000845"
     CDS             complement(11575..12519)
                     /gene="nikB"
                     /locus_tag="KLK57_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HBE0891298.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(12519..14093)
                     /gene="nikA"
                     /locus_tag="KLK57_000846"
     CDS             complement(12519..14093)
                     /gene="nikA"
                     /locus_tag="KLK57_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HBE0891299.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTPQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(14204..14791)
                     /gene="acpT"
                     /locus_tag="KLK57_000847"
     CDS             complement(14204..14791)
                     /gene="acpT"
                     /locus_tag="KLK57_000847"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HBE0891300.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(14846..15895)
                     /locus_tag="KLK57_000848"
     CDS             complement(14846..15895)
                     /locus_tag="KLK57_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE0891301.1"
                     /translation="MEIPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            16027..17244
                     /locus_tag="KLK57_000849"
     CDS             16027..17244
                     /locus_tag="KLK57_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0891302.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVNY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKVGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(17248..17805)
                     /gene="dcrB"
                     /locus_tag="KLK57_000850"
     CDS             complement(17248..17805)
                     /gene="dcrB"
                     /locus_tag="KLK57_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HBE0891303.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(17878..18543)
                     /locus_tag="KLK57_000851"
     CDS             complement(17878..18543)
                     /locus_tag="KLK57_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HBE0891304.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSILGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNILLKRLADKSEINAL
                     QAS"
     gene            18764..19009
                     /gene="tusA"
                     /locus_tag="KLK57_000852"
     CDS             18764..19009
                     /gene="tusA"
                     /locus_tag="KLK57_000852"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HBE0891305.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(19111..21309)
                     /gene="zntA"
                     /locus_tag="KLK57_000853"
     CDS             complement(19111..21309)
                     /gene="zntA"
                     /locus_tag="KLK57_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HBE0891306.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(21383..22009)
                     /locus_tag="KLK57_000854"
     CDS             complement(21383..22009)
                     /locus_tag="KLK57_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HBE0891307.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIIRSLYL"
     gene            22150..22509
                     /locus_tag="KLK57_000855"
     CDS             22150..22509
                     /locus_tag="KLK57_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HBE0891308.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(22512..22781)
                     /locus_tag="KLK57_000856"
     CDS             complement(22512..22781)
                     /locus_tag="KLK57_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HBE0891309.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(22771..23367)
                     /gene="rsmD"
                     /locus_tag="KLK57_000857"
     CDS             complement(22771..23367)
                     /gene="rsmD"
                     /locus_tag="KLK57_000857"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HBE0891310.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            23517..25013
                     /gene="ftsY"
                     /locus_tag="KLK57_000858"
     CDS             23517..25013
                     /gene="ftsY"
                     /locus_tag="KLK57_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HBE0891311.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVKAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVMVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            25016..25684
                     /gene="ftsE"
                     /locus_tag="KLK57_000859"
     CDS             25016..25684
                     /gene="ftsE"
                     /locus_tag="KLK57_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HBE0891312.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSHRMLTLSDGHLHGG
                     VGHE"
     gene            25677..26735
                     /gene="ftsX"
                     /locus_tag="KLK57_000860"
     CDS             25677..26735
                     /gene="ftsX"
                     /locus_tag="KLK57_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HBE0891313.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINDLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            26980..27834
                     /gene="rpoH"
                     /locus_tag="KLK57_000861"
     CDS             26980..27834
                     /gene="rpoH"
                     /locus_tag="KLK57_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HBE0891314.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            28105..29208
                     /gene="livJ"
                     /locus_tag="KLK57_000862"
     CDS             28105..29208
                     /gene="livJ"
                     /locus_tag="KLK57_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HBE0891315.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(29407..29790)
                     /gene="panM"
                     /locus_tag="KLK57_000863"
     CDS             complement(29407..29790)
                     /gene="panM"
                     /locus_tag="KLK57_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HBE0891316.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            30214..31323
                     /gene="livK"
                     /locus_tag="KLK57_000864"
     CDS             30214..31323
                     /gene="livK"
                     /locus_tag="KLK57_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HBE0891317.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            31371..32297
                     /gene="livH"
                     /locus_tag="KLK57_000865"
     CDS             31371..32297
                     /gene="livH"
                     /locus_tag="KLK57_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HBE0891318.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            32294..33571
                     /gene="livM"
                     /locus_tag="KLK57_000866"
     CDS             32294..33571
                     /gene="livM"
                     /locus_tag="KLK57_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HBE0891319.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVS
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTFFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            33568..34335
                     /gene="livG"
                     /locus_tag="KLK57_000867"
     CDS             33568..34335
                     /gene="livG"
                     /locus_tag="KLK57_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HBE0891320.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRNQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            34337..35050
                     /gene="livF"
                     /locus_tag="KLK57_000868"
     CDS             34337..35050
                     /gene="livF"
                     /locus_tag="KLK57_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HBE0891321.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            35334..36398
                     /gene="ugpB"
                     /locus_tag="KLK57_000869"
                     /pseudo
     CDS             35334..36398
                     /gene="ugpB"
                     /locus_tag="KLK57_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
     gene            36496..37383
                     /gene="ugpA"
                     /locus_tag="KLK57_000870"
     CDS             36496..37383
                     /gene="ugpA"
                     /locus_tag="KLK57_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HBE0891322.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSRQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALHSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            37380..38225
                     /gene="ugpE"
                     /locus_tag="KLK57_000871"
     CDS             37380..38225
                     /gene="ugpE"
                     /locus_tag="KLK57_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HBE0891323.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            38227..39297
                     /gene="ugpC"
                     /locus_tag="KLK57_000872"
     CDS             38227..39297
                     /gene="ugpC"
                     /locus_tag="KLK57_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HBE0891324.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNNEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            39294..40037
                     /gene="ugpQ"
                     /locus_tag="KLK57_000873"
     CDS             39294..40037
                     /gene="ugpQ"
                     /locus_tag="KLK57_000873"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBE0891325.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(40024..40464)
                     /locus_tag="KLK57_000874"
     CDS             complement(40024..40464)
                     /locus_tag="KLK57_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HBE0891326.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            40584..>42056
                     /gene="ggt"
                     /locus_tag="KLK57_000875"
     CDS             40584..>42056
                     /gene="ggt"
                     /locus_tag="KLK57_000875"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HBE0891327.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNIYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTV"
BASE COUNT         9957 a        10697 c        11606 g         9796 t
ORIGIN      
        1 taaatacgag agacatgata ttgaatgagt tgcatcaaca tgttttttta aaggacgatg
       61 cgataacccc ttgtatcttc cttggggatc atacaggcga tagatttagt actatttttg
      121 gtgataaata tatccttact ttgctaaatt ctatgcgaaa tatggatggt aataaagata
      181 gccgtattaa taaaaatgtc atcgttctgg ctggaaatca tgaaatcaat tttaatggta
      241 actatactgc acgactagaa aattataaac tctctgccgg tgatacgtat aacttaatta
      301 aaacgctcga tgtttgtcat tatgactcag aaacgaaagt tttaaccagt catcacggaa
      361 taataagaaa tgaagaaaat gaatgttatt gtctcggtgc tttgcaggtt ccatttaatc
      421 agatgaaaga cccaattgat cctgaggaat tagcaaatat atttaataaa aaacataaac
      481 aacacatgga tgatcgtctt atccatctaa ttagaagcaa tgctgtagca tccacacctg
      541 tatatgaaaa ttattttaat aacactactg ctttcagacc aaaacctgaa gatatcttca
      601 aatgtggaca gacgctcaaa aaaactaaac agaaatatgg ccattatgga ttaggggtag
      661 atcagcatca aaaaattgat aattatacca tggggctaaa ttcctggaag atagcaccta
      721 acgaacgtgg tgataaaaaa ggtgtcccag ggcttagttg ttttcaacct caataaaact
      781 tcatgagtgc tgtggtatct ctttttaatt ttgccagtat ttcataggtt ttcttaatcg
      841 attgtagagg catgttctga tgacgcctct acaatcttta gggactttct ctgctatgag
      901 ataagaaatt tcatattatg ctttaaggct gaatgatttg tgcaaaatat aattattttc
      961 ccatcgaaca tgaggtgtat gtcataagat taaatgttaa ctatgtattc ctcgcggtaa
     1021 ataatatgag gtaaaagatg aaaattggaa ctgtggcagg cactaacgac agtacaacga
     1081 caatcacaac aaatgatatg gtgcaagagc atgttaccaa cttcactaaa gaactgtttg
     1141 ggtatattgc aaatggtata ggtgatgata tatcaagtat tgccaggaca atgctcggtg
     1201 aggttgtgga aaaaatagat gattggcaaa tagaacggtt ccaacaatct attcaagatg
     1261 acaaaatttc atttaccata caaacgaatc attcagaaaa atatagtatg ctatcgggca
     1321 tgcgcgctca tattctacgt aggaataact gttatcagtt tattgtgaca attaatagta
     1381 aaaattacgg ttgccctctt gataataccg atattaattg gtgcagcatt gtttatttac
     1441 ttaataatat gacagtaaat gataatgcta atgatgttgc agttactgag tcgtacaaac
     1501 ccatctggaa ctggaaaata tcgcaataca atgtatttga tattaaattc gaaacaatga
     1561 taaaacccca gttcgctgac agaacctatt tctccaattg ttcacctgtt gatcccacaa
     1621 gtaccagacc gacctatttt ggcgataccg atggctcagt cggtgcagtt ttgtacgcgc
     1681 tttttgcaac gggacatttg ggaatcatgg ctgaaggaga aaatttcctt agccagttgc
     1741 ttaatataga agatgaagtt ctcaacgtat tgctaaggga gaactttaat gagcaacttg
     1801 acactaatgt taatataata atcagtatcc tgaacagaag agacattgtt ttagaaagtc
     1861 ttcagcctta tttggttatt aataaagacg cagtcacgcc atgtacgttt ctgggcgacc
     1921 agactggcga tcgatttagt aatatctgtg gcgataaatt tatcatcgat ttattaaaac
     1981 gcataatgag tatcaatgaa aacgttcatg tattagctgg aaatcatgaa acgaattgca
     2041 atggtaacta tatgcagaac ttcacgcgca tgaaaccact tgatgaggat acctactctg
     2101 ggataaaaga ttatcctgta tgtttctatg accccaaata caaaataatg gcaaatcatc
     2161 atgggataac ttttgatgat cagcgaaaac gttacattat aggaccgata acagtctcta
     2221 gtgatgaaat gaccaatgct ctcgatccgg tcgaattagc ggcaattatc aataaaaagc
     2281 accatgctgt aattaatggc aaaaagttta aaaatagccg ggctatctct tgtcgatcct
     2341 ttaatcgtta tttttctgtt tcgacagatt acagacctaa acttgaagca ctattagcat
     2401 gctcacaaat gttaggtata aatcaagttg ttgcacataa cggtaatggt ggaagagaat
     2461 gtataggtga aacggggaca gttttaggtc ttaacgcccg tgacagtaaa catgctggaa
     2521 gaatgttcag catgcataat tgccaaatta accctggtgc agggcctgag ataaccactc
     2581 cctggaagtc ttaccagcat gaaaaaaaca gaaatggact tatgcctttg atcagaaggc
     2641 gcacaatgtt acagctctga accatagaca acatgaatat taatgttact atctggttga
     2701 gactgttgat aaaacgtaaa aaggaatgct ctgttcctgc aactgaacag gcgtgaaacg
     2761 ctatccaaca ggatggatac cgttttgtca gccagtttta tagatcacac ttatgagtat
     2821 gcgtgtttca tttacggcca ggaatgctga tgatgacaac attactaccc gttttcacta
     2881 agccttcccc gttggcgctc aatgctctgc gcgctggtcg tatttgtcgt ttccttctta
     2941 tcccggatgg aagaatccgc tgaacgtcgt tcggcggcag ttgtaacacg cgtcgtttgc
     3001 ttgcagggat tatggcgggt cgattatgga taagagtaag cgccatctgg cgtggtgggt
     3061 tgtcgggtta ctggtggtgg ctgccgtcgt ggcgtggtgg ctgttgcgcc cggcgggtgt
     3121 gccggaaggc tttgctgtca gcaatgggcg cattgaagcg acggaagtgg atatcgccag
     3181 caaaattgcc gggcgtatcg acaccattct ggtgaaagaa ggccagtttg ttcgcgaagg
     3241 cgaagtgctg gcgaagatgg atactcgcgt gttgcaggag cagcgactgg aagccatcgc
     3301 gcaaatcaaa gaagcacaaa gtgccgttgc tgccgcgcag gctttgctgg agcaacggca
     3361 aagcgaaact cgtgccgcgc agtcgctggt taatcaacgc cagtccgaac tggactccgt
     3421 ggctaaacgt catacgcgtt cccgctcgct cgcccaacga ggggctattt ctgcgcaaca
     3481 gctggatgac gatcgcgccg ccgctgagag cgcccgagct gcgctggaat cggcaaaagc
     3541 tcaggtatcg gcttctaaag cggctataga agcggcccgt accaatatca ttcaggccca
     3601 aactcgcgtc gaagccgcgc aagccactga acggcgtatt gccgcagata tcgatgacag
     3661 tgaactgaaa gccccgcgtg acggacgcgt gcagtatcgg gttgctgagc caggggaagt
     3721 gctggcggca ggcggtcggg tgctgaatat ggtcgatctc agcgacgtct atatgacttt
     3781 cttcctgcca accgaacagg cgggcacgct gaaacttggc ggtgaagccc ggctgatcct
     3841 cgatgccgcg ccagatctgc gtattccggc gaccatcagt tttgtcgcca gtgtcgccca
     3901 gttcacgcca aaaaccgtcg aaaccagcga tgaacggctg aaactgatgt tccgcgtcaa
     3961 agcgcgtatc ccaccggaat tactccagca gcatctggaa tatgtcaaaa ccggtttacc
     4021 gggcgtagcg tgggtgcggg tgaatgaaga actttcgtgg cctgacgacc tcgtggtgag
     4081 gttgccgcaa tgacgcatct ggaactggtt cccgtcccgc ctgtcgcgca actggcgggc
     4141 gtgagccagc attatggaaa aaacgttgcg ctgaacaata ttactctcga tattccggcc
     4201 cgctgtatgg tcgggctgat tggtccggat ggcgtcggga agtcgagctt attgtcgttg
     4261 atttccggtg cccgcgtcat tgagcagggc aatgtgatgg tgctgggcgg cgatatgcgc
     4321 gacccgaggc atcgccgcga cgtctgcccg cgcatcgcct ggatgccgca ggggctgggc
     4381 aaaaacctct accacacctt gtcggtgtat gaaaacgtcg attttttcgc tcgcctgttc
     4441 ggtcacgaca aagcggagcg ggaagtgcga attaatgagc tgctgaccag cacggggtta
     4501 gcaccgtttc gcgatcgtcc ggcagggaaa ctctccggcg ggatgaagca aaaacttggg
     4561 ctgtgctgcg cgttaatcca cgacccggaa ctgttgatcc ttgatgagcc aacaacgggg
     4621 gttgacccgc tctcccgctc ccagttctgg gatctgatcg acagtattcg ccagcggcag
     4681 agcaatatga gcgtgctggt cgccaccgcc tatatggaag aggccgaacg cttcgactgg
     4741 ctggtagcga tgaatgccgg agaagtgctg gcaactggca gcgccgaaga gctacggcag
     4801 caaacgcaaa gcgctacgct ggaagaagca tttataaatc tgttaccgca agcgcaacgc
     4861 caggcgcatc aggcggtagt gatcccaccg tatcaacctg aaaacgcaga gattgccatc
     4921 gaagcgcgcg atctgaccat gcgttttggt tccttcgttg ccgttgatca cgttaatttc
     4981 cgcattccac gcggggagat ttttggtttt cttggttcga acggctgcgg taaatccacc
     5041 accatgaaaa tgctcaccgg actgctgccc gccagcgaag gtgaggcgtg gctgttcggg
     5101 caaccggttg atccaaaaga tatcgatacc cgccgtcggg tgggctatat gtcgcaggcg
     5161 ttttcgctct ataacgaact caccgtgcgg caaaaccttg agttacatgc ccgtttgttt
     5221 cacatcccgg aagcggaaat tcccgcaaga gtggctgaaa tgagcgagcg ttttaagctc
     5281 aacgacgttg aagatgttct gccggagtca ttgccgctcg gcattcgcca gcggctttcg
     5341 ctggcggtgg cggtgattca tcgcccggag atgttaatcc tcgatgagcc tacttctggt
     5401 gtcgatccgg tggcgaggga tatgttctgg cagttgatgg tcgatctctc gcgccaggac
     5461 aaagtgacta tcttcatctc tacccacttt atgaacgaag cggaacgttg cgaccgcatc
     5521 tcactgatgc acgccggaaa agtgcttgcc agcggtacac cgcaggaact ggttgagaaa
     5581 cgcggagccg ccagtctgga agaggcattt atcgcctatt tgcaggaagc ggcagggcag
     5641 agcaacgaag ccgaagcgcc gcccgtggta cacgacacca cccacgcgcc gcgtcaggga
     5701 tttagcctgc gccgtctgtt tagctacagc cgccgcgaag cgctggaact gcgacgcgat
     5761 ccagtacgtt cgacgctggc gctgatggga acggtgatcc tgatgctgat aatgggttac
     5821 ggcatcagta tggatgtgga aaacctgcgc tttgcggtgc tcgaccgcga ccagaccgtc
     5881 agtagccagg cgtggacact caacctctcc ggttcccgtt actttatcga acagccgccg
     5941 ctcactagtt atgacgagct tgatcgtcgg atgcgtgcgg gcgatatcac ggtggcgatt
     6001 gagatcccgc ccaatttcgg gcgcgatatc gcgcgtggta cgcctgtgga actcggcgtc
     6061 tggatcgacg gagcgatgcc cagtcgtgcc gaaacggtaa aaggttacgt gcaggcaatg
     6121 caccagagtt ggcttcagga tgtggcgagc cgacaatcga cacccgccag ccaaagcggg
     6181 ctgatgaaca ttgagacgcg ctatcgctat aacccggacg taaaaagcct gccagcgatt
     6241 gttccggcgg tgatcccgct tctgctgatg atgatcccgt caatgctaag cgcccttagc
     6301 gtggtgcggg aaaaagagct agggtcgatt atcaaccttt acgtgacccc caccacccgt
     6361 agcgaatttt tgcttggcaa acagctgccg tacatcgcgc tggggatgct gaactttttc
     6421 ctgctctgcg gcctgtcggt gtttgtgttt ggcgtaccgc ataaaggcag tttcctgacg
     6481 ctcaccctgg cggcgctgct gtatatcatc attgccaccg gaatggggct gctgatctcc
     6541 acctttatga aaagccagat tgccgccatt ttcggaacgg cgattatcac gttgatcccg
     6601 gcgacacagt tttccgggat gatcgatccg gtagcttcgc tggaagggcc tggacgttgg
     6661 atcggcgagg tttacccgac cagtcatttt ctgactatcg cccgcggaac gttctcgaaa
     6721 gcgctggatc tgactgattt gtggcaactt tttatcccgt tgctgatagc catcccgctg
     6781 gtgatgggct taagcatcct gctgctgaaa aaacaggagg gatgatgcgc catttacgca
     6841 atatttttaa tctgggtatc aaagagttgc gcagtctgct cggtgataaa gcgatgctga
     6901 cgctgattgt cttctcgttt acggtgtcgg tgtattcgtc agcgaccgtt acgccaggat
     6961 cgttgaacct cgcgccgatc gccattgccg atatggatca atcgcagtta tcgaaccgga
     7021 tcgttaacag cttctatcgt ccgtggtttt tgccaccgga gatgatcacc gccgatgaga
     7081 tggatgccgg actggacgcc ggacgctata ccttcgcgat aaatattccg cctaattttc
     7141 agcgtgatgt cctcgccgga cgccagccgg atattcaggt gaacgtcgat gccacgcgca
     7201 tgagccaggc atttaccggc aatgggtata tccagaatat tatcaacggt gaagtgaaca
     7261 gctttgtcgc gcgctaccgt gataacagcg aaccgttggt atcgctggaa acccggatgc
     7321 gctttaaccc gaacctcgat cccgcgtggt ttggcggggt gatggcgatc atcaacaaca
     7381 ttaccatgct ggcgattgta ttgaccggat cggcgctgat ccgcgagcgt gaacacggca
     7441 cggtggaaca cttactggtg atgccgataa cgccgtttga gatcatgatg gcgaagatct
     7501 ggtcgatggg gctggtggtg ctggtggtat cgggattatc gctggtgctg atggcgaaag
     7561 gtgtactggg cgtaccgatt gaaggctcga tcccgctgtt tatgctgggc gtggcgctca
     7621 gtctgtttgc caccacgtca atcggcattt ttatggggac gatagcgcgt tcaatgccgc
     7681 aactggggct gctggtgatt ctggtgctgc tgccgctgca aatgctttcc ggtggatcca
     7741 cgccgcgcga aagtatgccg cagatggtgc aggacattat gctgaccatg ccgacgacac
     7801 actttgttag cctcgcgcag gccattctct accggggtgc cggattcgaa atcgtctggc
     7861 cgcagttcct gacgctgatg gcaattggcg gcgcgttttt caccattgcg ctgctgcgat
     7921 tcaggaagac gattgggaca atggcgtaag gaagggaaac ttgacttcat actgtgatgt
     7981 catactattg atgtcataaa gtgaagtcat aaggacatgc catgagaacc aaggtacagg
     8041 ctttacggaa gaaaaaaaaa tacattggat caaatattta aaactcctgt tcctcaaggg
     8101 atcaagtggt ctgatataga gtcactggtt aaagcattag gcggagaaat taaggaagga
     8161 agaggttcgc gttgtaagtt catactaaat atgagcgttg cgtgtttcca tcggcctcat
     8221 ccgtcgccag ataccgataa aggcgctgta gaaagcgtgc gtgactggtt actaagtata
     8281 ggggtaaaac catgatcaaa ctgaagacgc caaactctat ggagattgcc gggcaaccag
     8341 ctgttattac ctacgtgcca gaactgaatg catttcgtgg caaattcctg ggcttgtcag
     8401 gttattgtga ttttgtctct gacagcattc aggggctgca aaaagaaggg gaactttccc
     8461 tgcgagagta tcttgaggat tgtaaagctg cgggtattga gccgtatgcg cgtacagaaa
     8521 agattaagac ctttacgtta cgctatccag aatcgctgag tgaacgcctt aataatgcgg
     8581 ctgcgcagca acaagtctct gttaatacct atattattga aacactgaat gagcgtttga
     8641 atcatttata aattttagcg gcgtacatta cgcagaatat ctgcctttac aggatctatc
     8701 tatgcaactg aacttaattt tataaattaa gttcagttaa tatcttttct ttcctcgttt
     8761 cagaaataac tcaatcttcc ttcggcaagc actgcaaatg cccatgccgc acgccgcgct
     8821 gggcgataac gtcatcggca aaatgctgca cgtcgcccat atcacctttc agcacggcga
     8881 tttccagaca gtcgtcgtga ttgatatgca catgcagcgt ggcgacggag agatcgtggt
     8941 gatggtgctg ggtggagaca atgcggctgg ctaagtcgcg tttttcgtgt tcatacacat
     9001 aggagagcac cgcgaaacct tgcgtgccgt gctgctgagt ggcctcctgg gccagggcgc
     9061 tacgcagaat atcgcggata gcttcggaac ggttgttata accacgacgc tggctcaggc
     9121 tgtccagcgt ctccagtaaa tcgtcatcaa gcgtgatggt gactcgttgc atttgcgtta
     9181 aaccttttct gtggtgcgac ggcgcacggg gaatgcgggt aataccgcgt tttgtagcac
     9241 acgtccggcg tcagaggaaa aggttaattt atctcccacc acctgggttt cgacgatttg
     9301 tccgttgtcc atcaccatca cccgctgaca gaagcgttcc accaggcgta agtcgtgggt
     9361 gatgaacagg caggcggtgc caaactgttg ttgtagcttt ttcagcaggc gaatgacgcc
     9421 cgcctgtaac acgagatcaa ggttagaaac ggcttcatcc agaatcagta gtttcggttc
     9481 gaccgccagc gcgcgagcca ggcagacgcg ctggagctgg ccgccgctta actgcggtgg
     9541 gcgtttgtcg agaacgctgt catcgagatc gaccgccttc agcatttcgc tggcgcgcgc
     9601 cagttgttcg gattttttca gtgatagcag gtggcgcatc ggttcacgca ggatctcgcg
     9661 cacggttttg cgcggattca cggcgctgat ggagtcctga aataccatct gaatatcgcg
     9721 gcggaacgct ttacgctggg cgcgattgag tttcgccagc ggttcgccac gccagctaat
     9781 attcccctgc gcgggcgatt ctaaacccac cagcaaccgc gcgagggtac ttttcccgca
     9841 gccgctgcgc cccagcaggg cgacggtttc gccgcttttc agggtcaggg aaacgttatt
     9901 cagcaccgcc tgatgttgat gttttccgtt aaatccaccg tgggcatagt gatgggaaag
     9961 gccggagacg ttaagtaaag tcatgatgcc agctccatac cgtagagggc gagatgagcg
    10021 gaaaccaggc tgcgcgttac cgcatgtttg ggggcgttaa acagcgtttc tacatcgccc
    10081 tgttcgacaa ttttaccttg tgacatcacc gccacgtcat ccgccagacg cgccacaacg
    10141 cccatatcat gggtgaccag cagcattccc ggcgcttgtt tttgcataat gctttccagc
    10201 agatcgagga tgcgcgcctg tgctaccacg tcgaggtcgg tggtcggttc atcggcgatg
    10261 ataaacggtg attcacacag caccgccatc gcaatcatca tgcgctgcaa catgccgccg
    10321 ctcatctcga acgggtacag cttcagcacg cgcgcggcgt tttccagccc caccgcttct
    10381 atggcagcgg taagcgtagc gtcatcggcg ggtttcccta acgccaggca ggtttcccgc
    10441 gcgtgggtgt gcatggtgtg tagcggatta aaggcgctgc gcgggttctg catgatggtg
    10501 gcaattttga tgccgcgcag ggcgcaggga taaaccgctt tgccatcggc taaaatttcc
    10561 cccgccgtct ggcgaacgcc agcgggcaaa atgcccagcg tcgcggcgca ggttaatgat
    10621 ttcccgctgc cgctaccgcc gactaacgcc agcacgcgcc cgcgttgcag ggttaacgat
    10681 acaccgtgta ccagcggctg cgcggcctgt agcgcgatat tacgtagttc aatctgttgc
    10741 ggcattagtg tgcgtgctcc gtcaccagat gaggatccag atgatcgcgc agtgcgtcac
    10801 ccaccaggtt aaaggccatc acgctgataa acagcgccag ccccggccag aacatttgca
    10861 gcggctgggt ccagatatac tggcgcgcgt cgttaatcat cacgccccat tcggcggtcg
    10921 gcgcggtcac accgaggcca aggaaagaca tccccgcgac gtgcagcatc atatggccga
    10981 tatccagcgt tgccagcacc agcagcgaag ggatcaccgc gcctgccaga tgatcgacaa
    11041 acacccgcac atggcccgca ccggaaagcc gtgacgccag cacaaactcg cgttggcgta
    11101 gtgaaatcac caagctgcgc accatgcgtg cataccacgc ccagtgcgac agggcgatgg
    11161 cgataattac gttggtcagc ccggtgccga gcacgccaac cataaagaac gacagaatcg
    11221 aggtcgggaa ggtcataaac atatcggcga cgcgcatggt ggcctgatca acgcgcccgc
    11281 caatcaaccc ggcgctgccg ccaataacca gccctaatgt cagcaccagc agcaggcagg
    11341 ccattaccga accgagcgac acgcgggtcg ctgccatcag ccgcgagaaa atatcgcgac
    11401 ctaagtgatc ggtgcccagc cagtgctgcg catccggcga aagcaggcgc gacggcaaat
    11461 caatcgcctg ggggtcatac ggcagccacc actggctggt gagcgcaatc agcgccagca
    11521 gggcgataat gatcagcgcc aggcgtaccg accagcggga agagaggaaa aagttcacgc
    11581 gtgcgctcct tcatgacgac gaatgcgcgg gtccagcgcg gcgttgagca aatcgacaat
    11641 caaattacag accacaaaaa ccaccaccat catcagcgta aagcactgaa tcaccggata
    11701 gtcacggtta aaaatcgccg acaccgcata gcgcccgacg cccggccagg caaagatgtt
    11761 ttcgataatc atcgtcccgc caatcagttc gccgatgtgc atccccacgg cggtgatcat
    11821 cggcagcgag gcattgcgca gaatatggcg gcgttcggtc tgtttgtcgt tcaggccgcg
    11881 cagacgcgcc caagtgacgt gacgctgacc ggcgacgtcc agcatactgg cgcgcagcag
    11941 tcgcgcgtta atcgccagcg acataaaggc aatggaaacc gcaggcaaaa tgatgtgctg
    12001 ccagccgccg taacccatcg cgggtagcca ttgcagatac accgaaaacg ccatcaccag
    12061 taaaaacgcc agccagaagt taggcatcga cacgccaaga aacgcgatga aacgtacggc
    12121 gaaatccggc agacggtcgc gatggcgcgc cgcccagata ccgagcggta cggaagtgag
    12181 cagaattaat accagcgccg cacctgcaag ttccagcgtg gcgggcagga agttcagcat
    12241 atcgtccagc accgggcgtt ggctggcgaa tgagatacca aagtcaagat gcagcgcctt
    12301 ccacaaccag gtaccgtact ggacgtacag cggctgatcc agcccgagca tggtgcgggt
    12361 agaggccagc atctccggcg tcggcggcag gttagataaa cgcagataat cgagcgccgg
    12421 gtcgccggta ccgaggcgca gcatcagaaa aatgatcacc gaggcggcga gcaccatcgg
    12481 gatcagcagc agaaagcggc gtaatacgta acgcaacatt aaggtttcac cggtttaatc
    12541 tgttcgaacg gaatttcggt ggcgatcggc gcgtagggga tattacccag ctccggtttt
    12601 gataccacca tcatcgagat gtagctgata ggcagataaa ccgcctcgtc atgcagacgg
    12661 gtcaaaatgt cgcgatacag cgcctgacgt tgcgtttcgt catgggtcgc cagcacttcg
    12721 ccgatctctt tatcaatcag cggcttgtcg gctaatcctt gctgtgcctg gaagtcagcg
    12781 tgcgacggta cgcgcattga actcaggaag gcgtgtggat cgtatggcgc gccccaggtg
    12841 cggtggaaaa tcatgccaaa acgaccgtcg cgctgacgag catagatact gctctcttct
    12901 tcgccaatca gcgagacatc tgcgccaatc tggcgcatat cagcctgaat gatttccgcc
    12961 atcgatttgc ttaacgcatc ggtgccgatg aacgacagtt caatgcgcag cggctgacca
    13021 tttttctcgc ggatgtcttt gcccgcaggc agcgtccaac cggctttttc cagcaactct
    13081 ttcgctttct gcggatcgta ctggcgcggt ttcaggccga gattagcgta aggcacagaa
    13141 ggggcaaaca gggtgtcggc gacctgcggg gtgccatata acgcgttatc aatcagcgat
    13201 tttttgttta ccgcgtaatt aagagcttca cgtactgcca gctcgttggt gggggctttg
    13261 gcggtattga gcgccagcat cacggtttcg atcggctgtg acagttgggt gtggtaagcc
    13321 ggattctggc taaagcgggc gaaggtatcg agcggtaata acccttcgtt tccgtacagc
    13381 aggtcgatat cgccagtttc aaacgccacc gcgcgggtag tcgggtccgg gatgacgtta
    13441 aaggtgatct ttttaatcgc tggcttttcg ccccagtagt tttcgttacg gacgaagaca
    13501 tcgtactgat tcagtttcga ttcctgcaaa atccacggtc cggtgccaat cggcgcttta
    13561 attccgttca tggtttcatg gtttttaaac tgcgagggag cgataaaacg gaaaggacgg
    13621 ggcagggcca gttcttgcag gaaaggatag taggcgcttt tcagggtaat ttgcagctct
    13681 gttttactga gtgctttaac atcaacaatc tggtttgcca gctccagcca ggcgtgacgt
    13741 tgacggttat cgagcactgc gcggaagttt tctgccgccg cctcggcatc gaacggttca
    13801 ccgttggaga atttcacgtc atcacgcagg gtgaaggtcc aggttttacc atcttctgaa
    13861 tgagtccagc tttttgccag ccacgggatc accgaaccgt ctgcctgata tttcaccaat
    13921 ggttcataaa ccatgctctg ggcgaacatc tggttaggcg tgtaaaggtg cgggtttagt
    13981 ggcccgacat tcaccggcca ggcggtagtg atttcatctg gtgcagcggc atggacgata
    14041 aaagacgcac aagccagcag cgcaaataga gtgcggcgga gtgtggagag catggtaacc
    14101 ccaatggatt aaaatagatg gcggaaataa gtatgacgat tttaagtatt cgtcatactg
    14161 attacctgtt ctggatcaat agatgggcag tcgggtgggc ggatcagtta actgaatcga
    14221 tccattgcac actgtcggcg gtgagcgtaa agggggtggg ggtgcagatc gcaaggctta
    14281 aattttctag ctgacaatgg ctgactgaca gcgaggagtg ataggtgcta tctacgctga
    14341 cgatttgcca ggcgctgccg ccacgctgtt taacgatggc ttctttgcgc gtccagatgc
    14401 gccagaacat ttccagttgc tgatcaggat gcacggcgtc catttcagcg tgttccccga
    14461 ggctgaacac ggcgttcgcc agccagcgcc agttggcgcg cgggcgaatc acttcgatat
    14521 cgcagccgac ttcgccttca tcgctcaaca gcagagcgat atcgtcaccg ctatggctta
    14581 agttgaacca tagcggcgtt tccggcgcga atgcaggttt gccttgttcg ccatagatga
    14641 tctccggtag cggggaaagc gtgtgcgaaa gcaatgcacg ccccgccagc cagcgttcgc
    14701 gtcgtggacc ttgcggtgct tgctcgcgta aacccggtgg cagtggagct gcgcttaagg
    14761 tcgaaacttt ccccagaact atccgataca tatcagggcc aacgtttaat ggaaaatgaa
    14821 agtgcgtatc gtatcacttg tcgcctcatc ccggtaaccg acttttcggt ctgcctggcc
    14881 ccagtaaaat cgccagtttg ctaccgcctt tggtggtttc catccagatt ttacacacac
    14941 tggttaatgg caccgaaagt agcatcccta ccgggccgag cagccatccc caaattaaca
    15001 atgaaagaaa taccaccatg gtggacatcc ccaggcgatg gcccatcatc cgtggttcta
    15061 aaatattgcc gatgaccata tggaccacta aaaacaatgc gccgaccaga atacattcgt
    15121 aaacaccatt aaacagcagc acctgaatca ttggcggtac ggcggaaatt accgcgccga
    15181 tattgggcac gtagttgagc aaaaacgcca gtactgccca catcagcgca aactgcaccc
    15241 ccatcagctc cagccccagc cagacgatga cacctgtcca taaactgagt agcgtcttca
    15301 atgcaagata gtgcgaaacg cctttaagtg cgcggtgtaa tcctgcgatg tgaatctgtg
    15361 ggttattcag cgcaaaacgc attttgtaag ggacgtggcg cacttcaaac agcataaaaa
    15421 ctacggtcat caccagcaaa agcacgctcg ccattgcccc ggaaagcccg gtcattagcg
    15481 ctgtggtgaa ggtaaccact ttttccgagt ccatccgctg cagcattcgc tccggcgaca
    15541 tatgcaaatt aagaaaaggc aacatctcct gcaatttaaa aagtttgcgc gtcagctcct
    15601 tattaaactt cggcagcata gagataaatt cgttaaacga tgccgccagt acgccgacca
    15661 gcgcggttag tgcgatcagc atcaccacca ctacaatcgt aatggcaacg gggcgttgta
    15721 ctccccgacg aataaaccag gtaacgagcg ggttgaggac gatggcaaaa aacagcgcca
    15781 gtaacagctg cacaatgata tctgccgctg cgtgaatgcc cgcgaggatc actaccagcg
    15841 aggccagctt gagcagaatg tgcatgcccg ttttatcggg ttgagggatt tccattgggg
    15901 cttccttgtg actttttgta ttaagtgtag cgggagtcac gccagcaata ttctgattct
    15961 tactgctgat tttcacgtca gcacgtggta aaaatgaaac actgttgtaa aaatgtggtg
    16021 atcctcatgc ccgaacccgt agccgaaccc gcgctaaacg gattgcgcct gaatttgcgc
    16081 attgtctcca ttgtcatgtt taacttcgcc agctacctca ccatcgggtt gccgctcgct
    16141 gtattaccgg gctatgtcca tgatgtgatg ggctttagcg ctttctgggc aggattggtt
    16201 atcagcctgc aatatttcgc caccttgctg agccgtcctc atgccggacg ttacgccgat
    16261 ttgctgggcc ccaaaaagat tgtcgtcttc ggtttatgcg gctgcttttt gagcggtctg
    16321 ggatatctga cggcaggatt aaccgccagt ctgcccgtca tcagcctgtt attactttgc
    16381 ctgggacgcg tgatccttgg gattgggcaa agttttgccg gaacgggatc gaccctgtgg
    16441 ggcgttggcg tggttggctc gctgcatatc gggcgagtga tttcgtggaa cggcattgtc
    16501 aattacgggg cgatggcgat gggtgcgccg ttaggcgtcg tgttttatca ctggggcggc
    16561 ttgcaggcgt tagcgttaat cattatgggc gtggcgctgg tggccatttt gttggcgatc
    16621 ccgcgtccga cggtaaaagc cagtaaaggc aaaccactgc cgtttcgcgc ggtgcttggg
    16681 cgcgtctggc tgtacggtat ggcactggca ctggcttctg ccggatttgg cgtcatcgcc
    16741 acctttatca cgctgtttta tgacgctaaa ggttgggacg gtgcggcttt cgcgctgacg
    16801 ctgtttagct gtgcgtttgt cggtacgcgt ttgttattcc ctaacggcat taatcgtatc
    16861 ggcggcttaa acgtggcgat gatttgcttt agcgttgaga taatcggcct gctactggtt
    16921 ggcgtggcga ctatgccgtg gatggcgaaa gtcggcgtct tactggcggg ggcagggttt
    16981 tcgctggtgt tcccggcatt gggcgtagtg gcggtaaaag cggttccgca gcaaaatcag
    17041 ggggcggcgc tggcaactta caccgtattt atggatttat cgcttggcgt gaccggacca
    17101 ctggctgggc tggtgatgag ctgggcgggc gtaccggtga tttatctggc ggcggcggga
    17161 ctggtcgcaa tcgcgttatt actgacgtgg cgattaaaaa aacggcctcc ggaacacgtc
    17221 cctgaggccg cctcatcatc ttaaaactta ctgaataacc agcgtattaa tgatgttttc
    17281 tgcggtggtc tgcgcttttt gctgatcgtc agctggcaga gtaatttgca tggtcagcag
    17341 ttgattaccc acgttaccca gaataacgga agagtacgcc gtctggcctt tcgcggagat
    17401 aatactgtct aactgctgca ttttgtgacc tttcagctca atggctttgt tggttaccac
    17461 ttgcagctgc ggatcgcggc tacgttgctg atcttccaga cgcttcgcca acaccgccag
    17521 atcttctttc ggatcatcgc ccatgatgac gatgactgct ttctgcccgg tggcgtcgga
    17581 ccagacatgc atgttattgg cctgcgttcc cagcttaccg ctctggtcgg tcatatccgc
    17641 tggcagcgag aaacttaact tgccatcaag caggttgacg ggattcccgg tagcgttact
    17701 ttccgcgacc gaaccctgcg ccgtagcgtt agtgtcttta tcatcacagg ccgcaagccc
    17761 cataaccagc aggccaattc cgacatattt aaccagattg cgcattgact tcttcctttc
    17821 gataaacggc cataacggct cattcatcca tcttatcaca actctgataa cgaaccttta
    17881 actcgcctgc aaagcgttga tttcggattt atctgccagt cttttcaaca gaatattgag
    17941 taatacgcca tacattggca ggaagaaaac gatactgatt aacactttga aacagtaatc
    18001 gaccagcgcg atttccatcc agtgttcagc cataaaggca tccgggctac gccagaaggc
    18061 aatgaagaaa aaggccagcg tgtcgctgac gttaccgaac agtgtggacg ctgtcggtgc
    18121 cagccaccag cggcgactct gacgcaggcg gttaaaaacg tgcacgtcga ggatctgtcc
    18181 cagcgcgtag gccatgaaac tggcggtggc gatacgggcg acaaacaggt tgaagtgggc
    18241 gagtgcgccg aatccctgcc aggaacccat atagaatagc gacgagatga cgtaggagat
    18301 cagcaacgcc gggatcatca ccgcaaagat gatacgacgg gccagcggtg cgccaaaaat
    18361 acgcacggtc aggtcggtag caaggaaaat aaacgggaag ctaaacgcgc cccaggtggt
    18421 atggaaaccc aaaatggaga cgggaagctg aaccagatag ttactggagg tgatcaccag
    18481 cagatgaaat aacgataacc agaacaacgc cttatagcgt tgagtttgcg agaaaacgtt
    18541 catattgtac ctttttgatt aaccattggg gtgagggaac ccaatacgta cgacacgtct
    18601 gttaccttaa cataataacg tgcttaccct tttttcgagc cgccgcatga tactgcttta
    18661 cgttgacaat gcaatggtta attttcacgc aatcgttaac ctggtttgct tacggactca
    18721 cagggcgtaa actagcgccg tttttttatg tgatgagaag aaaatgaccg atctcttttc
    18781 cagccctgac cacacactcg acgcacttgg cctgcgctgc ccggaacccg taatgatggt
    18841 gcgcaaaact gtgcgcaata tgcagcctgg cgaaacgttg ctgattatcg ccgacgatcc
    18901 ggccactacc cgcgatattc ctgggttttg tacctttatg gaacacgaac tggttgcgaa
    18961 agagacggat ggactgcctt atcgttattt gattcgtaaa ggcggttgat ggggaaagat
    19021 tggcttcgat gccgcctttt cccctcaccc taaccctctc cccagagggg cgaggggacc
    19081 gatcgcgctc aatgttgcga tcggtttgcc ttatctcctg cgcaacaatc ttaacgcatt
    19141 cgctgtcacc agcaccgtcg cacccgtatc tgccagcacc gccagccaca ggccggtcat
    19201 ccctaacagc gtggtgacga ggaagatccc tttcagcccc agcgcaatgg tgatgttctg
    19261 gcggatattg gcgtgagtgg cgcgtgccag ttcaatcatt tgcaccaggc cgcgcaggtg
    19321 gttatgggtt aatgctgcat cagcggtttc cagcgccacg tctgtgccac tgcccattgc
    19381 aatcccaatg gcggcagctt tcatcgctgg cgcgtcgtta ataccatcac cgaccatcgc
    19441 caggggcgca tgttgattca gcttggtcac cgctttgact ttatcttccg gcaataggcc
    19501 cgctttaaac tccagcccca gctccccggc gattgccgcc gctgcacgtg gattatcgcc
    19561 ggtgaggatc actcctttga cccccagcgc gttcagttca ctgatggcag ttgcagcatc
    19621 ggcgcgcagg gtatcctgca atgcaatgat acccagcacg tcatcattac gtactaccag
    19681 cacaaccgtt tgcccggcgc tttccagttc attaatcaaa ccagcaaatg catcagcggg
    19741 atgtttcccg gcagcgcaaa tcaatacgcg ctcaccgtta acctgcgctt caatgccaga
    19801 cccgaccagc gcccgctgtg attcggcggt gggaatggcg agttcagcaa cctgtgcttc
    19861 gcgtacgatg gcttgcgcca gtggatgcgt cgcgccttgc tcgaccgccg ccgccagtgt
    19921 cagcagttca gattcactaa tacccgttgc cggatgaatc gcggtaacgc gcggtttacc
    19981 gacggtcagc gtaccggttt tatcaaacgc cacctgagta acacgaccta gttgttccag
    20041 cgccgctccg cctttaatca acgccccacg acgcgctgcc gccgccagcc cggaggtaat
    20101 cgccgcaggc gttgagataa ctaacgcaca cgggcagcca atcagcagca gcgtcagccc
    20161 tttataaatc cactcctgcc agctggcggc aaacagcagc ggtggcacca gcgtcaccag
    20221 cagagcgacg gccataatcg cgggcgtata gatacggctg aaacggtcga taaaccgctc
    20281 aatgggggcg cgacgctctt cggcttcttc gatcagtttc agaatccggt caatggcgct
    20341 ggcacccggt tctgacagca cttccagcgt taccagacgg tctacgctgg tggctcctgc
    20401 aggaacttta tcgcccgtcg cgcgctccac cggaatggat tctccggtca gggcgctttc
    20461 atcaaaactg gcaaacggtg agagcagttt accgtcggca ggcaaacgcc cacctgcggc
    20521 gacttcaatc acatcgccag gccgcaggct gttaatcgcc acctcttccc gctcaccgtt
    20581 acgcaggcgc gtggcggttt ctggtttcag cgccattaac gcgctaacgc cctgacgcgc
    20641 gcggctggcg gcccagcctt ccagtcgttc accaatcaaa aacagcagca acaccatcgc
    20701 agcttcagcc gttgcgccaa taaacagtgc accaatagcg gctacgctca ttaaggtttc
    20761 aatggcgaag tagctgccgg atttgatcag ccgtaatgcc tgacgagcaa tcgggtacag
    20821 cccaaccagc gtggtcgcga taaacgccag ttgcccgaac ggatgattga actgctccag
    20881 accccagctg attgccatca tcacgattag tgtaatcagc ggcagattct ctttcaggcg
    20941 cgatgcttgt ggctcgtcgg cggcctgctc atcgcgcagg gaatagcccg ctttttgcac
    21001 cgcagattca acttgtgcac gaatgtcatt gtcggcatca accaccagtt tttcggtggc
    21061 gaacaacacc tgcacctgat tcacacctgc aagctggcgc acggcatttt ctaccttgcg
    21121 cgcacaggcg gcgcagtcca tgccactgac tttccagcta tagcgggtgc cggagacgtt
    21181 ttcagagagc gttggcgagc tggagcatgc gccgtcgcag caacagtcgt tggcgttctg
    21241 taccgtggtt agcggtttga acgcagcaaa ttgaggggct ttcttgccgt gattgtcagg
    21301 tgtcgacatg gcatactccg gttaagtttt ttctcattaa ccgaaggata cactctggag
    21361 tcgactccag agtcaagttt tatcagagat acagtgagcg gatgatgagg aagtgaccgg
    21421 caaagtagca ggccgcagca atcgcgttat ccgcacggaa gcgtcggcga tagtggctcc
    21481 ccagccagac aaagttactg ataaacagca acgaagcgcc aacaaacgca gagagcgccg
    21541 gagcggtcgg acggaagaac cacagttcac ctgccagcca caccatcacc agcgtcatgc
    21601 cgataaacgt gcagataggc caacgatact cttccagccg cgtccagata atcgccagta
    21661 acagcgcacc cagaaccagc aacaccagtg gcagaggcca gaagaaagag agcgtcatct
    21721 gactggcgaa atagatggtg tacagcaggt gcgagaggaa aaacgcgccg atggcgtaca
    21781 tcagacgttg acgtggcaac agggttagcg catcgcccag cagtgaggcg cacagccctg
    21841 ccagcaccag atagctaatg gcgtcgaaca tcggcgcttg ccaggccagt aacagcagga
    21901 gaagaagggt taacggttta aacacccagc gttgccaggt tggcccacga tacgatgcat
    21961 ccacagatag ccatgcggaa agacagacag cgataaacga ccaaagcatc ttagttcctt
    22021 gaattcgtta ttttttcgta agcggaatgc ctacggtctt gcttccagtt tagtggcgag
    22081 ggtggacgga tgacaacacc ataactggca gggtatgctt atttccgcat tttccgatga
    22141 gggataaaga tgagcaaacc acctcttttc tttattgtta tcattggctt aattgtcgtc
    22201 gccgcatcgt ttcgttttat gcaacagcga cgggaaaaag ctgataatga tatggctccg
    22261 ctccagcaaa agctggtggt ggtgagcaac aagcgggaaa aaccgattaa cgatcgccgt
    22321 tcgcgccagc aggaagtgac tccggcaggc accagtatgc gctacgaggc aagcttcaaa
    22381 ccgcaaagcg gaggaatgga gcagacgttt cgcctcgacg cccagcagta ccacgccctg
    22441 acagtgggcg ataaaggtac gctgagctat aaaggaacgc gctttgttag ctttgttggc
    22501 gaacaataag gttatttttt aactttaaat ttcttctgcc agaccagcag ttcaaacacg
    22561 ccgaaaagga aaatccgtac cttttcggcg gtggtcatct gcgggccatc tttcggtaaa
    22621 gtggatttca atagcgccag ctgcatacca tgcatcagca cggtaaaaat cagcgccacg
    22681 ttaacgaaaa tatttagcgg gcgcgggaag ggatgcacca gattgaggat taaaaatccc
    22741 caaacgcaga gcattaacaa acgaccaata ttaatcagca tcactttctc cttgtgcttc
    22801 gcgttgatac agccgataag ccacctgacc cgccactttt tcccgatgta acgaccagtt
    22861 tgccggaacg gtgggcagac cgttttcgac ttcgctttcg acataaatca gggcttcgtc
    22921 agccagccag ccgttatctt ccagtaaatt tatcgtctct tctaacaagc cacggcggaa
    22981 cggtggatcg acaaacacga tattatgcgg tgtgcctttt tgcgccagga atgacatcgc
    23041 gttgctgttc accacgcgtg cattgcctgc ttttagtgtc gccagattct taattaactg
    23101 ctgagaaact gcacgatcca tctcaattaa cgtggccccc gcagcgtagc gcgataacgc
    23161 ttccagcccc agcgcgccgc tcccggcgaa gcaatccaga cattgggcgt caacaatgac
    23221 cggagccagc cagttaaaca acgtttcgcg tacgcggtcg gtggtggggc gcagacctgg
    23281 gctatcagga accgggagtt tacggcctcg ccactgcccg ccaataatgc ggatttggcc
    23341 gctgccggaa tgattcggtt ttttcatgat aaattgctca atccgccaga taacacatac
    23401 ttgcaggcgg tgatgtgaat taagttaagt gatagactat ttcatcattt ttttagctgc
    23461 tatgtacata gcgttaacgc tgtgccatga agcaacagcg aggagtgtag tcgcaaatgg
    23521 cgaaagaaaa aaaacgtggc tttttttcct ggctgggctt tggtcaaaaa gagcagaccc
    23581 cggaaaaaga gacagaagtt cagaatgaac aaccggttgt agaagaaatc gttcaggcgc
    23641 aagagcctgt gaaggcttcg gaacaagccg ttgaagagca gccgcaggcg catactgaag
    23701 ccgaggcgga aacttttgct gccgacgttg tggaagtcac tgaacaggtt gttgaaagtg
    23761 aaaaagcgca gcctgaagcg gaagttgttg cacagccgga accggtcgta gaagaaacgc
    23821 cggagccagt ggctatcgaa cgtgaagagc tgccgttgcc ggaagacgtc aacgccgaag
    23881 aggtttcgcc agaagagtgg caggctgaag cggaaaccgt agagattgtc aaagcggcgg
    23941 aagaagaagc ggctaaagaa gaaatcaccg acgaagagct ggaagcacag gcgctggcag
    24001 ccgaagcagc agaagaggcg gtgatggtgg ttcctccggc agaagaagag cagccggtgg
    24061 aagaaatcgc tcaggagcag gaaaaaccaa ccaaagaagg tttcttcgcg cgcctgaaac
    24121 gcagcctgtt aaaaaccaaa gaaaatctcg gttccggatt tatcagcctg ttccgcggta
    24181 aaaaaatcga cgatgatctg tttgaggagc tggaagagca gcttttgatc gccgatgtgg
    24241 gcgtggaaac cacacgtaaa attatcacca atctgacgga aggcgcatcc cgcaagcagc
    24301 ttcgtgatgc cgaggcgctc tatggcctgc tgaaagaaga gatgggtgag attctggcga
    24361 aagtcgatga gccgctgaat gttgaaggta aaacgccgtt cgtgatcctg atggtgggcg
    24421 tcaacggtgt gggtaaaacc acgacgattg gtaagctggc acgtcagttt gagcagcagg
    24481 gtaaatcggt gatgctggcg gcgggcgata ctttccgtgc agcagcggtt gaacagcttc
    24541 aggtctgggg tcagcgcaac aatattccgg tgattgccca gcataccggg gcggattccg
    24601 cctctgttat cttcgacgcc attcaggcag ctaaagcgcg taatatcgac gtcctgattg
    24661 ccgataccgc cggacgcctg cagaacaaat cgcacctgat ggaagagttg aagaaaatcg
    24721 tccgcgtgat gaagaaactc gacgttgaag cgccgcatga agttatgctg actattgatg
    24781 ccagcaccgg gcagaacgcg gtaagccagg ccaaactgtt ccacgaagct gttggcttaa
    24841 ccggcatcac gctaacgaaa ctggacggca cggcgaaagg cggggtaatt ttctcggtgg
    24901 ctgaccagtt tggtatccct atccgctaca ttggtgtcgg cgaacgtatt gaggatttgc
    24961 gtccgtttaa ggcggacgac tttatagagg cactttttgc ccgagaggat taacaatgat
    25021 tcgctttgaa catgtcagca aggcttatct cggtgggaga caggcgctgc agggcgttac
    25081 gttccatatg cagccgggtg agatggcgtt tctgaccggt cattccggcg cagggaaaag
    25141 taccctcctg aagctgatct gtgggattga gcggcccagc gccgggaaaa tctggtttag
    25201 cggccatgac atcacgcgtc tgaaaaaccg tgaagttccg tttctgcgcc gccagattgg
    25261 catgattttc caggatcacc atctactgat ggaccgtact gtctacgata acgtggcgat
    25321 cccgctgatt atcgctggtg ccagcggtga cgatattcgt cgccgggtgt cggcggcact
    25381 ggataaagtc gggctactgg acaaagcgaa gaacttccct attcagcttt ccggcggtga
    25441 acaacagcgt gttggcattg cccgcgcggt ggtgaataag cccgcggtac tgctggcgga
    25501 cgaaccgacc ggtaacctgg acgacgcgtt gtcggaaggg attctacgtc tgtttgaaga
    25561 gtttaaccgc gttggggtaa ccgtattgat ggcaacgcac gacatcaacc tgatttcacg
    25621 gcgttcccat cgcatgctca ccctgagcga tggtcacttg catggaggcg tgggccatga
    25681 ataagcgcga tgcaatcaat catattcggc agtttggtgg gcgtctggat cgcttccgta
    25741 aatcggtcgg cggctcaggc gacggcggtc gtaacgcacc gaaacgcgcg aaatcctcgc
    25801 caaaaccggt aaatcgcaaa accaacgttt tcaacgaaca ggtgcgctat gccttccacg
    25861 gcgcattgca ggatctgaaa agcaaaccgt tcgccacgtt tttaacggtg atggttatcg
    25921 ccatctccct gacgctgccc agcgtctgtt atatggtgta caaaaacgtt aatcaggcgg
    25981 cgacgcagta ttatccgtca ccgcaaatca ctgtttactt gcaaaaaacg ctggacgatg
    26041 acgccgctgc gggcgtggtg gcacagttgc aggccgagca aggcgtggag aaagtgaact
    26101 atctttctcg tgaagacgca ctgggcgagt tccgtaactg gtctggtttt ggtggtgcgc
    26161 tggatatgct ggaagaaaac ccgcttccgg cagtggcggt ggtgatcccg aaactcgatt
    26221 ttcaggggac ggaatcactc aatacgctgc gtgatcgtat cacgcagatt aacggcattg
    26281 acgaagtgcg gatggatgac agctggtttg cccgtctggc ggcgttgacc gggctggtcg
    26341 ggcgcgtttc ggcgatgatc ggcgtgttga tggtggcggc agtgttcctc gtcatcggca
    26401 acagcgtacg tctgagcatc tttgctcgcc gtgactccat caacgtgcag aaactgattg
    26461 gtgcgacgga tggtttcatc cttcgcccgt tcctgtatgg tggcgcactg ctgggatttt
    26521 ctggcgcatt gttgtcatta attttgtcag aaattctggt gctgcgattg tcatcggcgg
    26581 ttgcggaagt ggcacaggtt ttcggaacga agtttgatat caatgattta tcgttcgatg
    26641 aatgcctgct attgctgctg gtatgctcga tgattggctg ggtggcagcg tggcttgcca
    26701 cggtacaaca tttacgccac tttacgcctg aataataaaa gcgtgttata ctctttccct
    26761 gcaatgggtt ccgtagcagg gaaagagatc ccgttgtctc ttcccggtat ttcatctcta
    26821 tgtcacattt tgtgcgtaat ttattcacaa gcttgcattg aacttgtgga taaaatcact
    26881 gtctgataaa acagtggatg ataacctcgt tgctcttaag ctctggcaca gttgttgcta
    26941 ccactgaagc gccagaagat atcgattgag aggatttgaa tgactgacaa aatgcaaagt
    27001 ttagctttag ccccagttgg caacctggat tcctacatcc gggcagctaa cgcgtggccg
    27061 atgttgtcgg ctgacgagga gcgggcgctg gctgaaaagc tgcattacca tggcgatctg
    27121 gaagcagcta aaacgctgat cctgtctcac ctgcggtttg ttgttcatat tgctcgtaat
    27181 tatgcgggct atggcctgcc acaggcggat ttgattcagg aaggtaacat cggcctgatg
    27241 aaagcagtgc gccgtttcaa cccggaagtg ggtgtgcgcc tggtctcctt cgccgttcac
    27301 tggatcaaag cagagatcca cgaatacgtt ctgcgtaact ggcgtatcgt caaagttgcg
    27361 accaccaaag cgcagcgcaa actgttcttc aacctgcgta aaaccaagca gcgtctgggc
    27421 tggtttaacc aggatgaagt cgaaatggtg gcccgtgaac tgggcgtaac cagcaaagac
    27481 gttcgtgaga tggaatcacg tatggcggca caggacatga cctttgacct gtcttccgac
    27541 gacgattccg acagccagcc gatggcaccg gtgctctatc tgcaggataa atcatctaac
    27601 tttgccgacg gcatcgaaga tgataactgg gaagagcagg cggcaaaccg tctgaccgac
    27661 gcgatgcaag gtctggacga acgcagccag gacatcatcc gcgcgcgctg gctggacgaa
    27721 gacaacaagt ccacgttgca ggaactggct gaccgttacg gtgtttccgc tgaacgtgta
    27781 cgccagctgg aaaagaacgc gatgaaaaaa ctgcgcgctg ctatagaagc gtaatttccg
    27841 ctattaagca gagaaccctg gatgcgagtc cggggttttt gttttttggg cctctacaat
    27901 aatcaatttc ccctccggca aaacgccaat ccccacgcag attgttaata aactgtcaaa
    27961 atagctattc caatatcata aaaatcgggt atgttttagc agagtatgct gctaaagcac
    28021 gggtagccat gcataaaacg aaataaagtg ctgaacaaca acatcacaac acacgtaata
    28081 accagaagaa tggggattct caggatgaac ataaagggta aagcgttact ggcaggatgt
    28141 atcgcgctgg cattcagcaa tatggctctg gcagaagata ttaaagtcgc ggtcgtgggc
    28201 gcaatgtccg gtccggttgc gcagtacggt gaccaagagt ttaccggcgc agagcaggcg
    28261 gttgcggata tcaacgctaa aggcggcatt aaaggcaaca aactgcaaat cgtaaaatat
    28321 gacgatgcct gtgacccgaa acaggcggtt gcggtggcga acaaagtcgt taacgacggc
    28381 attaaatatg tgattggtca cctctgttct tcatcaacgc agcctgcgtc tgacatctac
    28441 gaagacgaag gcattttaat gatcacccca gcggcaaccg cgccggagct gaccgcccgt
    28501 ggctatcagc tgatcctgcg caccaccggc ctggactccg accaggggcc gacggcggcg
    28561 aaatatattc ttgagaaagt gaaaccgcag cgtattgcta tcgttcacga caaacagcaa
    28621 tacggcgaag gtctggcacg agcggtgcag gacggcctga agaaaggcaa tgctaacgtg
    28681 gtgttctttg atggtatcac cgccggggaa aaagatttct ctacgctggt ggcgcgtctg
    28741 aagaaagaga atatcgactt cgtttactac ggcggttacc acccggaaat ggggcaaatc
    28801 ctgcgtcagg cacgcgcggc agggctgaaa actcagttta tggggccgga aggtgtggct
    28861 aacgtttcgc tgtctaacat tgcgggcgaa tcagcggaag ggctgctggt gaccaagccg
    28921 aagaactacg atcaggttcc ggcaaacaaa cccattgttg acgcgatcaa agcgaaaaaa
    28981 caggacccaa gcggcgcatt cgtttggacc acctacgccg cgctgcaatc tttgcaggcg
    29041 ggcctgaatc agtctgacga tccggctgaa atcgccaaat acctgaaagc gaactccgtg
    29101 gataccgtaa tgggaccgct gacgtgggat gagaaaggcg atctgaaagg ctttgagttc
    29161 ggcgtatttg actggcacgc caacggcacg gcgacagatg cgaagtaatc attaatcggc
    29221 aactttgggt tgccgccaaa ttgctaatat cgagtacgtt gcttcatgcc ggatgcggcg
    29281 taaacgcctt atccggccta taagatccaa agaaatcagt aaattgcaac acacattgta
    29341 ggcctgataa gcgtagcgca tcaggcaatt tctcgttggt catcactttt gaaatcaact
    29401 tgacgattaa cgcttctccc agccgccctg ttgcgccgta aatcccagtg cctgcataaa
    29461 cgctgtcatc acaccgcgat cttccacgcc tgcatccgcc atccaccagc atgaaacgcc
    29521 aggattgtta cgcaaaacct cttccagcag atattgcccc acaccgcgac gtcgggtgac
    29581 ttcccgcaca cgcagggaat ccagtgctcc ctcggtgccg cttaaggtta cccgtacggc
    29641 agcgagcagg cgctcgttaa aacgcgcggc gtagatacgg tggttatcgt caacctgtaa
    29701 agaggaaggg gaatactccg gccagatctt ttgcaggtca atccggtcct ggtcgctaaa
    29761 gttttctaat cgaatgatgg tcagcttcat gggtaacccg tgtaaatcac aaaagtggaa
    29821 ccagtgtagc gaaataattt aatcggaggc tttctctttt ttatttcttt tggcaggtga
    29881 ttaatttttt aacagcaata attacaaagt taaaacatta gagaatgaaa aatacccagc
    29941 ataatcccct gaatggtagt gaattattcc gccctttgtg ccgttatttt atgctgacaa
    30001 aggcactttt ttctgtttat ctatcaataa attcagaata ttatctgttc ttaatcaact
    30061 gaaaaatagg gattttaatc actgttatca caaaatactg cgctaaaccc ttaatcagac
    30121 aggcaaaaac agtgcagtat aaaaaaagaa cagtctgatt tgttaacaca taaaaacaaa
    30181 gcaacacaac atcacgaatg gggatttttg actatgaaac ggaatgcgaa aactatcatc
    30241 gcagggatga ttgcactggc aatttcacac accgctatgg ctgacgatat taaagtcgcc
    30301 gttgtcggcg cgatgtccgg cccgattgcc cagtggggcg atatggaatt taacggcgcg
    30361 cgtcaggcga ttaaagacat taatgccaaa gggggaatta agggcgacaa gctggttggc
    30421 gtggaatatg acgacgcctg cgacccgaaa caagccgttg cggtcgccaa caaaatcgtt
    30481 aatgacggca ttaaatacgt tattggtcat ctgtgttctt cttctaccca acctgcatca
    30541 gatatctacg aagacgaagg tattctgatg atctcgccgg gagcgaccaa cccggagctg
    30601 acccaacgcg gttatcaaca cattatgcgt actgccgggc tggactcttc ccaggggcca
    30661 acggcggcaa aatacattct tgagacggtg aagccccagc gcatcgccat cattcacgac
    30721 aaacaacagt atggcgaagg gctggcgcgt tcggtgcagg acgggctgaa agcggctaac
    30781 gccaacgtcg tcttcttcga cggtattacc gccggggaga aagatttctc cgcgctgatc
    30841 gcccgcctga aaaaagaaaa catcgacttc gtttactacg gcggttacta cccggaaatg
    30901 gggcagatgc tgcgccaggc ccgttccgtt ggcctgaaaa ctcagtttat ggggccggaa
    30961 ggtgtgggta acgcatcatt gtcgaatatt gctggcgatg ctgccgaagg catgttggtc
    31021 actatgccaa aacgctatga ccaggatccg gcaaaccagg gcatcgttga tgcgctgaaa
    31081 gcagacaaga aagatccgtc cgggccttat gtctggatca cctacgcggc ggtgcaatct
    31141 ctggcgactg cccttgagcg tactggcagc gatgagccgc tggcgctggt gaaagattta
    31201 aaagctaacg gtgcaaacac cgtgattggg ccgctgaact gggatgaaaa aggcgatctt
    31261 aagggatttg attttggtgt cttccagtgg cacgccgatg gttcatccac ggcagccaag
    31321 tgatcatccc accgcccgtg aaatgcgggc gggtttagaa aggttacctt atgtctgagc
    31381 agtttttata tttcttgcag cagatgttta acggcgtcac gctgggcagt acctacgcgc
    31441 tgatagccat cggctacacc atggtttacg gcattatcgg catgatcaac ttcgcccacg
    31501 gcgaggttta catgattggt agctatgtct cctttatgat catcgccgca ctgatgatga
    31561 tgggcattga taccggctgg ctgctggtgg cggcgggatt cgtcggcgca atcgtcattg
    31621 ccagcgccta cggctggagt atcgaacggg tagcctatcg cccggtgcgt aactctaagc
    31681 gcctgattgc gctcatctcg gcaatcggca tgtccatctt cctgcaaaac tacgttagcc
    31741 tgaccgaagg ttcgcgtgac gtggcgctgc ccagtctgtt taacggtcag tgggtggtgg
    31801 ggcatagcga aaacttctct gcctctatta ccaccatgca ggcagtgatc tggattgtta
    31861 ctttcctcgc catgctggcg ctgacgattt tcattcgtta ttcccgtatg gggcgcgcct
    31921 gccgtgcctg cgcggaagat ctgaaaatgg cgagcctgct tggcatcaac accgaccggg
    31981 tgattgcttt gacctttgtt attggcgcgg cgatggcggc agtggctggc gtactgctcg
    32041 gtcagttcta cggcgtcatt aacccctata tcggctttat ggccgggatg aaagccttta
    32101 ccgctgcggt gctcggtggg attggcagca ttccaggggc gatgattggc gggctgatcc
    32161 tggggattgc ggaggcgctc tcttctgcct atctgagtac ggaatataaa gatgtggtct
    32221 cattcgccct gctgattctg gtgctgctgg tgatgccgac cggtattctg ggtcgcccgg
    32281 aggtagagaa agtatgaaac cgatgcatat tgcaatggca ctgctctctg ccgcgatgtt
    32341 ctttgtcctc gctggcgtct ttatgggcgt gcaactggag ctggatggca ccaaactggt
    32401 ggtcgacagc gcttcggatg tccgttggca gtgggtgttt atcggcacgg cggtggtctt
    32461 tttcttccag cttttgcgac ctgctttcca gaaagggcta aaaagcgttt ccgggccgaa
    32521 gtttattctg cccgcgattg atggctccac ggtgaagcag aaactgttcc tcgtggcgct
    32581 gctggtgctt gcggtgtcgt ggccgtttat ggtgtcacgt gggacggtgg atatcgccac
    32641 tctgaccatg atctacatta tcctcggtct cgggctgaat gtggttgttg gtctttctgg
    32701 tctgctggtg ctggggtacg gcggttttta cgccatcggc gcttacactt ttgcgctgct
    32761 caatcactat tacggtctgg ggttctggac ctgtttgccg attgccggac tgatggcagc
    32821 ggcggcgggc ttcctgcttg gttttccggt gttgcgtctg cgcggtgatt atctggcgat
    32881 cgttaccctc ggtttcggcg aaattgtgcg catattgctg ctcaataaca ccgaaattac
    32941 cggcggcccg aacggtatca gtcagatccc gaaaccgact ttcttcggcc ttgagttcag
    33001 ccgtaccgcc cgtgaaggcg gttgggacac gttcagtaat ttctttggcc tgaaatacga
    33061 tccctccgat cgcgtcatct tcctgtatct ggtggcgttg ctgctggtgg tgctaagcct
    33121 gtttgtcatt aaccgcctgc tgcggatgcc gctggggcgt gcgtgggaag cgttgcgtga
    33181 agatgagatc gcctgccgtt cgctgggctt aagcccgcgt cgtatcaagc tgactgcctt
    33241 taccatcagt gccgcgtttg ccggttttgc cggaacgctg tttgctgcgc gccagggctt
    33301 tgtcagcccg gaatccttca cctttgctga atcggcgttt gtactggcga tagtggtgct
    33361 cggcggtatg ggatcgcagt ttgcggtgat tctggcggca attttgctgg tggtatcgcg
    33421 cgaattgatg cgtgatttca acgaatacag catgttaatg ctcggtggtt tgatggtgct
    33481 gatgatgatc tggcgtccgc agggcttgct gcccatgacg cgcccgcaac tgaagctgaa
    33541 aaacggcgca gcgaaaggag agcaggcatg agtcagccat tattatctgt gaacggcctg
    33601 atgatgcgct tcggcggcct gctggcggtg aacaacgtca atcttgaact gtacccgcag
    33661 gagatcgtct cgttaatcgg ccctaacggt gccggaaaaa ccacggtttt taactgtctg
    33721 accggattct acaaacccac cggcggcacc attttactgc gcaatcagca cctggaaggt
    33781 ttaccggggc agcaaattgc ccgcatgggc gtggtgcgca ccttccagca tgtgcgtctg
    33841 ttccgtgaaa tgacggtaat tgaaaacctg ctggtggcgc agcatcaaca actgaaaacc
    33901 gggctgttct ctggcctgtt gaaaacgcct tctttccgtc gcgcccagag cgaagcgctc
    33961 gaccgcgccg ccacctggct tgagcgcatt ggtttgctgg aacacgccaa ccgtcaggcg
    34021 agtaacctgg cctatggtga ccaacgccgt ctggagattg cccgctgcat ggtgacgcag
    34081 ccggagattt taatgctcga cgaaccggcg gcaggtctta acccaaaaga aaccaaagag
    34141 ctggatgagc tgattgccga actgcgcaat catcacaaca ccactatctt gttgattgaa
    34201 cacgatatga agctggtgat ggggatttca gaccgaattt atgtggtcaa tcaggggacg
    34261 ccactggcaa acggtacgcc ggagcaaatc cgtaataacc cggacgtgat ccgtgcctat
    34321 ttaggtgagg cataagatgg aaaaagtcat gttgtccttt gacaaagtca gcgcccacta
    34381 cggcaaaatc caggcgctgc atgaggtgag cctgcatatc aatcagggcg agattgtcac
    34441 cctcattggc gcgaacgggg cggggaaaac caccttgctc ggcacgttat gcggcgatcc
    34501 gcgcgctacc agcgggcgaa ttgtgtttga tgataaagac attaccgact ggcagacggc
    34561 gaaaatcatg cgcgaagcgg tagcgattgt cccggaaggg cgtcgcgtct tctcgcggat
    34621 gacggtggaa gagaacctgg cgatgggcgg cttttttgcc gaacgcgacc agttccagga
    34681 gcgcataaag tgggtgtatg aactgttccc acgtctgcat gagcgccgtg ttcagcgggc
    34741 aggcaccatg tccggcggtg agcaacaaat gctggcgatt ggtcgcgcgc tgatgagcaa
    34801 cccgcgtttg ctgctacttg atgagccatc gcttggtctt gcgccgatta tcatccagca
    34861 aattttcgac accatcgagc agctgcgcga gcaggggatg actatcttcc tcgtcgagca
    34921 gaacgccaac caggcgctaa agctggcgga tcgcggctac gtgctggaaa acggccatgt
    34981 ggtgctctcc gatactggtg atgcactact ggcgaatgaa gcggtgagaa gtgcgtattt
    35041 aggcgggtaa taacacgttg attgataagg agtcaaaaga ctcctttgag acaggtgaca
    35101 aatgtaaaat tgcctgatgc gctgcgctta tcaggcctac tgggtgagtt gcaatatatt
    35161 gaatttgcac gatcttgtag gccggataag gcgtttacgc cgcatccggc atgaaacgat
    35221 gagtaatctg tagagtttga ttcagacctt ctatttttcc gcttatccgt gccccatctc
    35281 ccattttccc tcacccacgc cgtcaccgcc ttgtcatctt tctgacacct tacttgcagg
    35341 tttatgaagt tggcaccgcc accatgatgg catcgaaagc cattaaaccg gtatatgacg
    35401 tgtttaaaga ggcagggatt caattcgatg agtcgcagtt tgtgccgacg gtttccggtt
    35461 actactccga cagcaaaacg ggccacttac tctcccagcc attcaacagc tcgactcccg
    35521 ttctctatta caacaaagac gccttcaaga aagcggggtt agacccggaa cagccgccga
    35581 aaacctggca ggatctggcg gactcttccg caaaactgaa agcctccggt atgaagtgcg
    35641 gctacgccag cggctggcag ggctggatcc aactggaaaa ctttagcgcc tggaacggtc
    35701 tgccgtttgc cagcaaaaac aacggctttg acggcacgga cgcggtgctg gagttcaaca
    35761 agccggagca ggtgaaacac attgccatgc tcgaagagat gaacaagaag ggcgacttca
    35821 gctacgtcgg tcgtaaggat gaatccaccg agaagttcta taacggtgat tgcgcgatga
    35881 ccaccgcctc ttccggttct ctcgctaata ttcgtgagta cgccaaattt aactatggcg
    35941 taggcatgat gccttacgac gccgatgcga aagatgcacc gcaaaacgcc attatcggcg
    36001 gggccagcct gtgggtgatg cagggcaaaa ataaagaaac gtataccggc gtggcgaagt
    36061 tcctcgactt cctcgcgaag ccagaaaacg ctgccgagtg gcatcagaaa accggttatc
    36121 tgccaatcac caaagcagcg tatgacctga cccgtgagca gggcttttat gagaaaaacc
    36181 caggggcgga taccgcgacg cgtcagatgc tgaataagcc gccgttgccg ttcaccaaag
    36241 ggctgcgtct gggcaacatg ccgcagatcc gcgtgattgt ggatgaagag ctggagagcg
    36301 tgtggaccgg taagaagaca ccacagcagg cactggatac cgccgttgag cgtggaaatc
    36361 agttgctgcg ccgctttgag aaatcgacga agtcttaatc agtgtaatgt cggatgcgtt
    36421 tcgcttatct gacctggcat cgcgtgtagg ccggataagc gaagcgcatc cggcacagtt
    36481 caggaattaa ccgtaatgtc atcatcccgt ccggtgttcc gctcgcgctg gctgccttat
    36541 ctgctggtcg cgccgcagct catcatcacc gttatctttt ttatctggcc tgcgggcgaa
    36601 gcgttgtggt actcgcggca aagcgtcgat ccgtttggtt tctccagcca gtttgtcggt
    36661 ctggataact tcgtcacgct gttccatgac agctactatc tagactcctt ctggacgacg
    36721 ataaaattca gcacctttgt caccgtcagc ggtttgctgg tatcgctgtt ctttgcggcg
    36781 ctggtggagt acatcgtgcg cggcagccgt ttctatcaaa ccttgatgct gctgccgtat
    36841 gccgtggctc ccgccgttgc cgccgtattg tggatcttcc tgtttaaccc cggtcgcggg
    36901 ctgatcaccc attttctcgc tgagttcggc tacgactgga accacgcgca aaacagcggt
    36961 caggcaatgt ttctggtggt gtttgcctcg gtgtggaagc aaatcagcta caactttctg
    37021 ttcttctatg ccgcgctgca ctccattccc cgttcgttga ttgaagccgc agctattgac
    37081 ggcgcagggc cgattcgccg cttctttaag attgctctgc cgcttatcgc tccggtgagt
    37141 ttcttcctgc tggtggtgaa cctggtgtac gccttcttcg acaccttccc ggtgatcgac
    37201 gccgccacgt ccggcgggcc ggttcaggcc accactacgc tgatttataa gatctaccgc
    37261 gaaggcttta ccggactgga tctggcttcg tctgccgcgc agtcggtggt gttgatgttc
    37321 ctcgtcatcg tgctgacggt ggtgcagttc cgctatgttg aaagcaaggt gcgttaccaa
    37381 tgattgagaa ccgtccgtgg ctgacgatat tcagccatac catgctgatc ctcgggatcg
    37441 cggtgatcct cttcccgctg tacgtggcgt ttgtcgcggc gacgctggat aaacaggccg
    37501 tctatgccgc gccgatgacg ctcatccccg gcacacatct gctggaaaat atccacaaca
    37561 tctgggttaa cggggtaggc acgaatagcg cgccgttctg gcggatgttg cttaacagct
    37621 ttgtgatggc gttcagcatt acgctcggca aaattaccgt ctcgatgctc tcggcatttg
    37681 ccattgtctg gtttcgtttt ccgctacgta acctcttctt ctggatgatt tttatcaccc
    37741 tgatgctgcc ggttgaagta cgtatcttcc cgacggtgga agtcatcgcc aacctgaaaa
    37801 tgctcgacag ctacgccggt ttaacgctgc cgctgatggc ctcggcgacc gccacgtttc
    37861 tgttccgcca gttctttatg accctaccgg atgaattggt ggaagccgcg cggatcgacg
    37921 gcgcgtcacc aatgcgtttc ttttgcgaca tcgtttttcc gttgtccaaa actaatctgg
    37981 cggcgctgtt tgtgatcacc tttatctacg gctggaatca gtatttgtgg ccgttgttga
    38041 ttattaccga cgttgatctc ggcaccaccg tggcagggat caaagggatg atcgctacag
    38101 gcgaaggcac cacggaatgg aactcggtaa tggcggcgat gttgttaacg cttatccctc
    38161 cggtggtgat tgttttagtg atgcagcgtg ccttcgtgcg cggcctggtc gatagtgaga
    38221 aataagatgg caggactgaa attacaggca gtaaccaaaa gctgggatgg caaaacccag
    38281 gtcattaaac cgctgaccct tgatgtggcg gatggcgaat ttatcgtgat ggtcgggccg
    38341 tctggctgcg ggaaatcgac gctgctgcgc atggttgccg ggctggagcg ggtgacagaa
    38401 ggcgatatct ggatcaacga ccagcgcgtg accgaaatgg agccgaaaga tcgcgggatt
    38461 gcgatggtgt tccagaatta cgcgctttat ccgcatatga gcgtagagga gaacatggcg
    38521 tgggggctga aaattcgcgg catgggcaag cagcaaattg ccgagcgcgt taaagaagcg
    38581 gcgcgcattc tggaactgga cggtctgctt aagcgtcgtc cgcgtgagct atccggcggt
    38641 cagcgccagc gtgtggcgat gggccgcgcg attgtgcgcg atccggcggt gttcttgttt
    38701 gatgagccgc tctctaacct cgatgccaag ctgcgcgtgc agatgcgtct tgaactgcaa
    38761 cagttgcacc gtcgcctgaa aacgacttca ctctacgtta ctcacgatca ggttgaagcg
    38821 atgacgctcg cccagcgagt aatggtgatg aacggtggcg ttgccgaaca gattggcaca
    38881 ccagttgaag tctacgaaaa gcccgccagc ctgtttgtgg cgagttttat cggcagcccg
    38941 gcgatgaacc tgctgacagg ccgcgtgaat aatgaaggca cgcatttcga actggacggc
    39001 ggtatcgtgc taccgctaaa cggtggctac cgtcagtatg ccgggcgtaa aatgactctc
    39061 ggcattcgcc cggaacatat agcgctaagc tcgcaggcag aaggtggtgt gccgctggtg
    39121 atggacacgc tggagatcct cggcgcagat aacctggcgc acggacgctg gggcgagcag
    39181 aagctggtgg tgcgactggc gcatcaggag cgcccgacgg caggcagcac gctgtggctg
    39241 catttggcgg aaaatcagct gcatcttttt gatggtgaaa caggacaacg agtatgagta
    39301 actggcctta tccccgcatc gtcgctcatc gtggcggcgg taagctggcc ccggaaaaca
    39361 ccctggcggc aatcgacgtc ggggcaaaat acggtcataa gatgatcgaa tttgacgcga
    39421 agttatcgaa agatggcgag atcttcctgc tccatgacga caatctcgaa cgcaccagca
    39481 acggctgggg cgtcgcgggt gaactgaact ggcaggattt actgcgcgtg gatgcgggca
    39541 gttggtacag caaagcgttt aaaggtgagc cgctgccgtt gctttcgcag gtggcggaac
    39601 gctgtcgcga acacgggatg atggcgaata tcgaaatcaa acccaccact ggcactggac
    39661 cattaacggg caaaatggtg gcgctggcgg cacgccaact gtgggccggt atgacgccgc
    39721 cgctgctgtc atcgtttgag attgatgctt tagaagctgc acaacaggcg gcaccggaac
    39781 tgccgcgtgg tttattgctt gatgagtggc gcgacgactg gcgcgaactg accgcgcggc
    39841 tgggctgcgt ctctattcat ctcaatcata agttactcga taaagcgcga gtgatgcagt
    39901 tgaaagacgc cggattacgg attctggttt ataccgtcaa taaaccccag cgcgcggcag
    39961 agttgctgcg ctggggcgtg gattgcatct gtaccgatgc gattgacgtg attggtccga
    40021 actttacggc ccaatagttt tcaacggaat gtcaggctgc ggcgtgctgg gctgattcag
    40081 catgtcgccg tttctccttt ccggcagcat atgctgctga ttcaacgaac tatccggatt
    40141 acggttgctg tttaacatcc cgccgttggt gttgggcagc atttgctgcc gggcgggatt
    40201 tcgctccccc ggctgtgact gcaacacccg ctgagaatta ttgtttatct ggttttctaa
    40261 atgctgctgt tgcaactgag tttgcgtttt cagttgctga ttcagcatcc ctttttgctg
    40321 gatttgctga gtctgcatct gggtttgcat ccgctgctgg ctgggtatct gataccccgg
    40381 ctggttaggg ttgttcagag tattaatggg ctgtgcaaag ccgacaaacg gcaggagtgc
    40441 cgtaagaatc agaagtcgtt tcatcgcgta tcctcctctg aagatatcct ttaagtttac
    40501 tcgcttcccg acaaaacgat gattaattca gagttatata ccaggcttag ctggggttgc
    40561 cccttaatct ctggagaata acgatgataa aaccgacgtt tttacgccgg gtggccattg
    40621 ctgctctgct ctcaggaagt tgttttagcg ccgccgccgc gcctcctgcg ccgcccgtct
    40681 cgtatggtgt ggaggaagat gtcttccacc cggtacgcgc gaaacaggga atggtagcgt
    40741 ctgtggacgc cactgccact caggtggggg tggatatcct caaggagggc gggaatgccg
    40801 ttgatgccgc cgtggcggtg ggctacgcgc tggcggtaac gcatccgcag gcagggaatc
    40861 tgggcggtgg tggttttatg ttaatccgct cgaaaaatgg caataccacg gctatcgatt
    40921 tccgcgaaat ggcacccgcc aaagcgaccc gcgatatgtt cctcgatgat cagggcaacc
    40981 cggacagcaa aaaatcactc acttcgcatc tggcttccgg cacaccgggt acggtagcag
    41041 gtttctcgct ggcgctggat aaatacggca ccatgccgct gaacaaagtc gtgcagcccg
    41101 cgtttaaact ggcacgcgat ggttttatcg ttaacgacgc gctggctgac gatctcaaaa
    41161 cctacggtag cgaagtgttg ccgaatcacg aaaacagtaa agctatcttc tggaaagagg
    41221 gcgagccgct gaaaaagggc gacacgctgg tgcaggcgaa cctggcaaag agcctggaga
    41281 tgattgctga aaacggcccg gacgaattct ataaaggcac gattgcggaa cagatcgccc
    41341 aggagatgca gaaaaacggt ggcttgatca ctaaagaaga tttagcggcc tataaagcgg
    41401 tcgaacgcac tccgataagc ggcgattatc gcgggtatca ggtttactcc atgccaccgc
    41461 catcctccgg cgggatccat atcgtacaaa tcctcaatat tctggaaaac ttcgatatga
    41521 agaaatacgg ctttggcagc gccgatgcga tgcaaatcat ggcagaagcg gagaaatacg
    41581 cctacgccga ccgctcggaa tatcttggcg acccggattt tgtcaaagtc ccgtggcagg
    41641 cactgaccaa taaagcctat gccaaatcca ttgccgatca aattgatatc aacaaagcga
    41701 agccgtccag tgagattcgc cctggcaagc ttgcgcctta tgagagtaat caaactaccc
    41761 attactctgt agtggataaa gacggtaacg cggtggcggt gacctatacg ctgaacacca
    41821 ccttcggtac gggcattgtc gcgggcgaga gcggtattct gcttaataat cagatggatg
    41881 atttctccgc caaaccgggc gtaccgaaca tttacgggct ggtgggcggt gatgccaacg
    41941 ccgtcgggcc gaataaacgt ccgctgtcat cgatgtcacc aaccattgtg gtgaaagacg
    42001 gtaaaacttg gctggttacc ggtagtcctg gcgggagtcg gattattacc accgtg
//
LOCUS       DAEEKQ010000016        41010 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.016, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000016 DAEEKQ010000000
VERSION     DAEEKQ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 41010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.016"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            216..1880
                     /gene="asnB"
                     /locus_tag="KLK57_000876"
     CDS             216..1880
                     /gene="asnB"
                     /locus_tag="KLK57_000876"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HBE0891328.1"
                     /translation="MCSIFGVFDIKTDAVEQRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVHKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            2260..2336
                     /locus_tag="KLK57_000877"
     tRNA            2260..2336
                     /locus_tag="KLK57_000877"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2294..2296,aa:Met,seq:cat)
     gene            2345..2429
                     /locus_tag="KLK57_000878"
     tRNA            2345..2429
                     /locus_tag="KLK57_000878"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2379..2381,aa:Leu,seq:tag)
     gene            2453..2527
                     /locus_tag="KLK57_000879"
     tRNA            2453..2527
                     /locus_tag="KLK57_000879"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2485..2487,aa:Gln,seq:ttg)
     gene            2562..2636
                     /locus_tag="KLK57_000880"
     tRNA            2562..2636
                     /locus_tag="KLK57_000880"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2594..2596,aa:Gln,seq:ttg)
     gene            2652..2728
                     /locus_tag="KLK57_000881"
     tRNA            2652..2728
                     /locus_tag="KLK57_000881"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2686..2688,aa:Met,seq:cat)
     gene            2777..2851
                     /locus_tag="KLK57_000882"
     tRNA            2777..2851
                     /locus_tag="KLK57_000882"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2809..2811,aa:Gln,seq:ctg)
     gene            2888..2962
                     /locus_tag="KLK57_000883"
     tRNA            2888..2962
                     /locus_tag="KLK57_000883"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2920..2922,aa:Gln,seq:ctg)
     gene            complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="KLK57_000884"
     CDS             complement(3116..4291)
                     /gene="ubiF"
                     /locus_tag="KLK57_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HBE0891329.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPARIRQLQNMNMAQFQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            4437..5861
                     /gene="miaB"
                     /locus_tag="KLK57_000885"
     CDS             4437..5861
                     /gene="miaB"
                     /locus_tag="KLK57_000885"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HBE0891330.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGSTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            6014..7054
                     /locus_tag="KLK57_000886"
     CDS             6014..7054
                     /locus_tag="KLK57_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HBE0891331.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            7051..7518
                     /gene="ybeY"
                     /locus_tag="KLK57_000887"
     CDS             7051..7518
                     /gene="ybeY"
                     /locus_tag="KLK57_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HBE0891332.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            7608..8486
                     /gene="corC"
                     /locus_tag="KLK57_000888"
     CDS             7608..8486
                     /gene="corC"
                     /locus_tag="KLK57_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HBE0891333.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            8511..10049
                     /gene="lnt"
                     /locus_tag="KLK57_000889"
     CDS             8511..10049
                     /gene="lnt"
                     /locus_tag="KLK57_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HBE0891334.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEVQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(10127..12004)
                     /locus_tag="KLK57_000890"
     CDS             complement(10127..12004)
                     /locus_tag="KLK57_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HBE0891335.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCIGIFCYLAQWMSYEEVDQSA
                     LIHLGANVAPLTLSGEPWRLLSSIFLHSSVSHLLMNMFAFLVVGGVAEQILGKWRLLI
                     TWLFSGVFGGLISACYALRESEQIVISVGASGAILGIAGAAIATQFASGTGTYHKNQR
                     RVFPLLGMVALTLLYGARQTGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVPAIDLVTYQHVADIPLEKYDGTGTSGKPGAWV
                     MALSPDERTLLVPGAGRGNIVRINTITHQKEDFPAGDARGTISAMRFRPENGEVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQMDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            12459..13367
                     /gene="gltI"
                     /locus_tag="KLK57_000891"
     CDS             12459..13367
                     /gene="gltI"
                     /locus_tag="KLK57_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HBE0891336.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKDKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHSDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWEIVGKPQSQEAYGCMLRKDDPQFKKLMDDTTAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            13537..14277
                     /gene="gltJ"
                     /locus_tag="KLK57_000892"
     CDS             13537..14277
                     /gene="gltJ"
                     /locus_tag="KLK57_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HBE0891337.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            14277..14951
                     /gene="gltK"
                     /locus_tag="KLK57_000893"
     CDS             14277..14951
                     /gene="gltK"
                     /locus_tag="KLK57_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HBE0891338.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            14951..15676
                     /gene="gltL"
                     /locus_tag="KLK57_000894"
     CDS             14951..15676
                     /gene="gltL"
                     /locus_tag="KLK57_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HBE0891339.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            15794..16729
                     /gene="rihA"
                     /locus_tag="KLK57_000895"
     CDS             15794..16729
                     /gene="rihA"
                     /locus_tag="KLK57_000895"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HBE0891340.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            16821..18491
                     /gene="hscC"
                     /locus_tag="KLK57_000896"
     CDS             16821..18491
                     /gene="hscC"
                     /locus_tag="KLK57_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HBE0891341.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDDVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGVSGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASDYLAIEIP"
     gene            complement(18551..20002)
                     /gene="djlC"
                     /locus_tag="KLK57_000897"
     CDS             complement(18551..20002)
                     /gene="djlC"
                     /locus_tag="KLK57_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HBE0891342.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGKPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEQDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITQMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EDEKPQSITSIIKISLTVLMIGYALGKIAMLFS"
     gene            complement(19999..20706)
                     /locus_tag="KLK57_000898"
     CDS             complement(19999..20706)
                     /locus_tag="KLK57_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBE0891343.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEERKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            20808..21362
                     /locus_tag="KLK57_000899"
     CDS             20808..21362
                     /locus_tag="KLK57_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBE0891344.1"
                     /translation="MNKKSMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(21372..22799)
                     /locus_tag="KLK57_000900"
     CDS             complement(21372..22799)
                     /locus_tag="KLK57_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HBE0891345.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLLSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(22796..23503)
                     /locus_tag="KLK57_000901"
     CDS             complement(22796..23503)
                     /locus_tag="KLK57_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HBE0891346.1"
                     /translation="MDMGSQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSS
                     WGIDDRDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFV
                     ASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYSNWRQ
                     YFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYPALSWQP
                     LADYPTCPETLKDMSDL"
     gene            23667..24644
                     /locus_tag="KLK57_000902"
     CDS             23667..24644
                     /locus_tag="KLK57_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBE0891347.1"
                     /translation="MVFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWTLDRGEAPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(24714..25196)
                     /locus_tag="KLK57_000903"
     CDS             complement(24714..25196)
                     /locus_tag="KLK57_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HBE0891348.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            25431..28013
                     /gene="leuS"
                     /locus_tag="KLK57_000904"
     CDS             25431..28013
                     /gene="leuS"
                     /locus_tag="KLK57_000904"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HBE0891349.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNMLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVLVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            28028..28609
                     /gene="lptE"
                     /locus_tag="KLK57_000905"
     CDS             28028..28609
                     /gene="lptE"
                     /locus_tag="KLK57_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HBE0891350.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            28609..29640
                     /gene="holA"
                     /locus_tag="KLK57_000906"
     CDS             28609..29640
                     /gene="holA"
                     /locus_tag="KLK57_000906"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HBE0891351.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVTARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQTQLRQAVQLLTRTELTLKQDYGQSVWAELKGLSLLLCHK
                     PLADVFIDG"
     gene            29642..30283
                     /gene="nadD"
                     /locus_tag="KLK57_000907"
     CDS             29642..30283
                     /gene="nadD"
                     /locus_tag="KLK57_000907"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HBE0891352.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            30307..30918
                     /locus_tag="KLK57_000908"
                     /pseudo
     CDS             30307..30918
                     /locus_tag="KLK57_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
     gene            31178..31495
                     /gene="rsfS"
                     /locus_tag="KLK57_000909"
                     /gene_synonym="rsfA"
     CDS             31178..31495
                     /gene="rsfS"
                     /locus_tag="KLK57_000909"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HBE0891353.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            31499..31966
                     /gene="rlmH"
                     /locus_tag="KLK57_000910"
     CDS             31499..31966
                     /gene="rlmH"
                     /locus_tag="KLK57_000910"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HBE0891354.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            31997..33898
                     /gene="mrdA"
                     /locus_tag="KLK57_000911"
     CDS             31997..33898
                     /gene="mrdA"
                     /locus_tag="KLK57_000911"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HBE0891355.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNAPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            33901..35013
                     /gene="mrdB"
                     /locus_tag="KLK57_000912"
                     /gene_synonym="rodA"
     CDS             33901..35013
                     /gene="mrdB"
                     /locus_tag="KLK57_000912"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HBE0891356.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            35024..36112
                     /gene="rlpA"
                     /locus_tag="KLK57_000913"
     CDS             35024..36112
                     /gene="rlpA"
                     /locus_tag="KLK57_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HBE0891357.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDSVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            36251..37462
                     /gene="dacA"
                     /locus_tag="KLK57_000914"
     CDS             36251..37462
                     /gene="dacA"
                     /locus_tag="KLK57_000914"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HBE0891358.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            37572..37835
                     /locus_tag="KLK57_000915"
     CDS             37572..37835
                     /locus_tag="KLK57_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HBE0891359.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            37936..38577
                     /gene="lipB"
                     /locus_tag="KLK57_000916"
     CDS             37936..38577
                     /gene="lipB"
                     /locus_tag="KLK57_000916"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HBE0891360.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            38837..39790
                     /locus_tag="KLK57_000917"
     CDS             38837..39790
                     /locus_tag="KLK57_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HBE0891361.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            39999..40964
                     /gene="lipA"
                     /locus_tag="KLK57_000918"
     CDS             39999..40964
                     /gene="lipA"
                     /locus_tag="KLK57_000918"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HBE0891362.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
BASE COUNT        10300 a        10448 c        10290 g         9972 t
ORIGIN      
        1 ccccgtaaaa tataaccatt caataaaacc cgctaaaaat tatcgattca tcaataaata
       61 catcctcaca ccagaccctt tttcaccaat cgacaatcac cattacgttt tttatttttt
      121 caccgccaaa tcgcttgcgc acagtgctgc tttgcggcat ttttttaaac aagcaaacac
      181 aacaagcaac aaataccagg ttaacggaga aggttatgtg ttcaattttt ggcgtattcg
      241 atatcaaaac agacgcagtt gagcagcgta agaaagcact cgagctgtca cgcctgatgc
      301 gtcatcgtgg cccggactgg tccggtattt atgccagcga taacgccatt ctcgctcacg
      361 aacgtctgtc aattgttgac gttaacgcgg gggcgcaacc tctctacaac caacaaaaaa
      421 cccacgtact ggcggtaaac ggtgaaatct acaaccacca ggcattgcgc gctgaatatg
      481 gcgatcgtta tcagttccag acagggtctg actgtgaagt gatcctcgcg ctgtatcagg
      541 aaaaagggcc ggaatttctc gacgacttgc agggcatgtt tgcctttgcc ctgtacgaca
      601 gcgaaaaaga tgcctacctg attggtcgcg accatctggg gatcatccca ctgtatatgg
      661 ggtatgacga acacggtcag ctgtatgtgg cctcagaaat gaaatcgctg gtgccagttt
      721 gccgcacgat taaagagttc ccggcgggga gctatttgtg gagccaggac ggcgaaatcc
      781 gttcttatta tcatcgcgac tggttcgact ttgatgcagt gaaagataac gtgaccgaca
      841 aaaacgagct gcgtcaggct ctggaagatt ccgttaaaag ccatctgatg tctgatgtgc
      901 cttacggtgt gctgctttct ggtggtctgg attcctcaat tatttccgct atcaccaaga
      961 aatacgcagc ccgtcgcgtg gaagatcagg aacgctctga agcctggtgg ccccagttac
     1021 actcctttgc tgtaggtctg ccgggttcac cggatcttaa agcagcccag gaagtggcaa
     1081 accatctggg cacggtgcat cacgaaattc acttcactgt acaggaaggt ctggatgcca
     1141 tccgcgacgt gatttaccac atcgaaactt atgatgtgac cactattcgc gcttcaacac
     1201 cgatgtattt aatgtcgcgt aagatcaagg cgatgggcat taaaatggtg ctgtccggtg
     1261 aaggttctga tgaagtgttt ggcggttatc tttacttcca taaagcgccc aacgctaaag
     1321 aactgcatga agagacggtg cataaactgc tggccctgca tatgtatgac tgcgcgcgcg
     1381 ccaacaaagc gatgtcagcc tggggcgtgg aagcacgcgt tccgttcctc gacaaaaaat
     1441 tccttgatgt ggcgatgcgc attaacccgc aggataaaat gtgcggtaac ggcaaaatgg
     1501 aaaaacacat cctgcgtgaa tgttttgagt catacctgcc cgcaagcgtg gcctggcggc
     1561 agaaagagca gttctccgat ggcgtcggtt acagttggat cgacaccctg aaagaagtgg
     1621 cggcgcagca ggtttctgat cagcaactgg aaactgcccg cttccgcttc ccgtacaaca
     1681 cgccaacctc aaaagaagcg tatctgtacc gggagatctt tgaagaactg ttcccgcttc
     1741 cgagcgccgc tgagtgcgtg ccgggcggcc cgtccgtcgc gtgttcttcc gctaaagcga
     1801 ttgagtggga tgaggcgttc aagaaaatgg acgatccgtc tggtcgtgcg gttggcgttc
     1861 accagtcggc gtataagtaa gaaatgcgaa acagccccgg tgaaatccgg ggctgtttca
     1921 gttattgagg tgcttgttga ttctgatatg cagtaaggaa atgttccggg tttgccacat
     1981 cactgtttga caactcatca ctgtaggcca gtatttcata gattaatttc ccaccaccat
     2041 tgttgaattc cgaatttcat cctgtatgac aaaagaatgg ctgtagggtg gggaaaaaac
     2101 acaaagtaaa caataattga cgaatatagc gccacgctgt tcgcaaccta accaaacagt
     2161 cactttcgag caattttcct tgaaaaagag gttgacgctg caaggctcta tacgcataat
     2221 gcgccccgca acgccgataa ggtatcgcga aaaaaagatg gctacgtagc tcagttggtt
     2281 agagcacatc actcataatg atggggtcac aggttcgaat cccgtcgtag ccaccatctt
     2341 ttttgcggga gtggcgaaat tggtagacgc accagattta ggttctggcg ccgcaaggtg
     2401 tgcgagttca agtctcgcct cccgcaccat tcaccagaaa gcgttgtacg gatggggtat
     2461 cgccaagcgg taaggcaccg gtttttgata ccggcattcc ctggttcgaa tccaggtacc
     2521 ccagccatct tcttcgagta agcggttcac cgaccggtta ttggggtatc gccaagcggt
     2581 aaggcaccgg tttttgatac cggcattccc tggttcgaat ccaggtaccc cagccatcga
     2641 agaaacaatc tggctacgta gctcagttgg ttagagcaca tcactcataa tgatggggtc
     2701 acaggttcga atcccgtcgt agccaccaaa ttctgaatgt gtcgaatacg ttcggcaaat
     2761 tcaaaaacca atttgttggg gtatcgccaa gcggtaaggc accggattct gattccggca
     2821 ttccgaggtt cgaatcctcg taccccagcc aatttattca agacgcttac atcgtaagtg
     2881 caccagttgg ggtatcgcca agcggtaagg caccggattc tgattccggc attccgaggt
     2941 tcgaatcctc gtaccccagc cacattaaaa aagctcgctt cggcgagctt tttgcttttc
     3001 tgcgttcatt caatgtcgaa tgcgatgttg acacgtctta tccttcaatg tcggatgcga
     3061 cgctgccgcg tcttatccga cctacggttg gcacgcatcc ggcaatgttg taaggctaca
     3121 accctaacgc atatttcagc gcctgacgtt tcaacacgcc agcacgctct gccgccatta
     3181 acccgagatt acgcacaaaa cgcagcggcg gcagattatt actgaatccg gcataaaaca
     3241 gatccatacc actttgcata atgaagttat ccgccatgcg ccgcatctgg taacgcttga
     3301 ggacaggata actggcccac gcttcaccgt agctgcgggc attcaccaga acatcaatca
     3361 gggcatcgac atcacgataa ccaagattca ccccctgccc cgccagcgga tggatggtat
     3421 gcgcggcatc gcccaccagc gcaagccccg gctgcacata ctgcaacgca tgtcggcgcg
     3481 tcagcggaaa cgcaccagcg gcaagcggtg taacgtaacc cagacgcgac gggaaatgct
     3541 tcgcgatttc cgcctggaat tgcgccatat tcatattctg caactggcga atacgcgccg
     3601 gagagtcata ccacaccagc gatgcccagt tatcaaacaa cggcagaaac gcacgcggtc
     3661 cgtccggggt aaattgctgc caggtgctgt cgccgggatc gttctcacac tgtacgctaa
     3721 tcaacatgca cgactgcgca tactgccagg catgaacgcc aattcccgcc atctgccgca
     3781 cctgagaatt tgcgccgtcg gcaccaatca ccagtttcgc gcggatcgtt tcaccgccct
     3841 tcagttccag ctcctgaaga tcattatctc gatgtaatgc aatcagcgag cctggcacac
     3901 gtaacgttac tttcggatgc gcttccagcg cctgccacag cgcctgttgc aggacggtgt
     3961 tttccaccat atagccaagc aacggtagct taagttcagc ggcgtcaaac accacatgcg
     4021 ccgtttccca ctcccacgtt tccagtctgc ggtaaggatg gcaacgcata gcctgtactg
     4081 catcccagac ccctaaccct ttaagcaatg ataccgatgc ggcgctgatc gccgagatcc
     4141 gcacgtccgg ttggctatca gcgacaaacg gtgcaggttc tgcgtgctcg atcaccgtta
     4201 ccgaaaatcc gtgctgtgcc agccccagcg ccagcgcgcc gccgaccatt cctccgccga
     4261 caatggcaat ttccgttggt tgatttgtca tggtcaatca tcctgttagc aatatgcttt
     4321 aagtttacag gatttttacc cccaagggct gataacgctc atactggtca caacggcagc
     4381 aaagcattac actatacgcc ctgcattcct ggctactatt tcgcaagagc aagtcgatga
     4441 ccaaaaaact ccatattaaa acctggggct gtcagatgaa cgagtacgat tcatcgaaga
     4501 tggccgatct gctggatgcc acccacggct atcaactgac cgacgtggcg gaagaagcgg
     4561 atgtgctgct gctgaacacc tgctcaatcc gcgagaaggc tcaggaaaaa gtcttccatc
     4621 agttgggtcg ctggaaactg ttaaaagaga agaatccaga cctgattatc ggcgtcggtg
     4681 gctgcgtggc atcgcaagaa ggcgagcaca ttcgccagcg cgcccactat gtcgatatta
     4741 tttttgggcc gcaaacgctg caccgtctgc cggagatgat caactccgtg cgcggcgacc
     4801 gcagcccggt tgtagatatc agcttcccgg aaatcgagaa gtttgaccgt ctaccggaac
     4861 cgcgcgccga agggccgacc gcgtttgtct ccatcatgga aggctgcaat aaatattgca
     4921 cctactgcgt ggtgccttac acccgtggtg aagaggtaag ccgtccgtct gacgatattc
     4981 tgtttgagat tgcccagctt gcggctcagg gcgtgcgtga agtcaacctg ctcggtcaga
     5041 acgtgaacgc ctggcgtggt gaaaactacg acggctccac cggatcgttt gccgatctgc
     5101 tgcgtctggt tgctgcgatc gacgggatcg atcgtattcg ctttaccacc agccatccga
     5161 tcgaattcac cgacgatatc atcgaagtgt atcgcgacac gccggagctg gtgagcttcc
     5221 tgcatctgcc agtacaaagc ggttccgatc gcattctgaa cctgatgggc cgtactcaca
     5281 cggcgctgga gtacaaagcg atcatccgta aactgcgtgc ggcgcgtccg gatattcaga
     5341 tcagttctga cttcattgtt ggcttccctg gcgaaaccac cgaagacttc gagaaaacga
     5401 tgaagctgat tgccgacgtc aatttcgaca tgagctacag ctttatcttc tctgcacgtc
     5461 cgggtacacc agccgccgat atggttgatg atgttccgga agaagagaag aagcaacgtc
     5521 tgtatattct gcaagaacgt atcaatcagc aagcgatggc atggagccgc cggatgctcg
     5581 gcaccaccca gcgtattctg gtagaaggga cttcgcgtaa gagcatcatg gagctttccg
     5641 ggcgtaccga aaataaccgc gtggtcaact tcgagggcac accggatatg atcggtaaat
     5701 tcgtcgatgt agaaattacc gacgtctacc cgaactctct gcgcggtaaa gtggtgcgta
     5761 ctgaagatga aatgggtctg cgcgtggcag aaacaccgga atcggtgatt gcccgtaccc
     5821 gcaaagaaaa cgaccttggc gtgggttatt atcagccgta attctcaggc ccgccgttcc
     5881 ggtgggcctt gttttttctc tctttatccc catctttatc gcaatgcttg cgcctttttc
     5941 ctgtggcgtg aataatttcc ttataggccg gtgaatgttc agcttccggc ggcatacaga
     6001 taagaggaac ggtttgaaca tagacactcg cgaaatcacc ctggagccag cagacaacgc
     6061 gcgtctgttg agcctgtgcg gcccgtttga tgacaacatc aagcagctcg aacgccgtct
     6121 cggcatcgag atcaatcgcc gcgacaacca ctttaaactg accggccgtc cgatttgcgt
     6181 caccgctgcg gcagacattc tgcgtagcct gtatgtcgat acagccccga tgcgcggtca
     6241 gattcaggat atcgaaccgg aacagatcca ccttgcgatt aaagaagcgc gagtactgga
     6301 gcaaagtgcg gagagcgtgc cggagtacgg caaagcggtc aatatcaaaa ccaaacgcgg
     6361 cgtaattaag ccgcgcacgc caaaccaggc gcagtacatc gccaatattc tcgaccatga
     6421 cattaccttc ggcgttggcc cggcgggtac gggtaaaacc tacctggcag tggctgcggc
     6481 agttgatgcc ctggagcgtc aggagattcg ccgtattctg ctgactcgtc cggcggtcga
     6541 agccggtgag aaactgggct tcctgcctgg cgatttaagc cagaaagtag acccgtatct
     6601 gcgcccactg tacgacgcgc tgtttgaaat gctgggcttt gagaaagttg agaaactgat
     6661 tgagcgcaac gttattgaag tcgcgccgct ggcctatatg cgtggtcgta cgctgaacga
     6721 cgcgtttatc attctcgatg agagccagaa caccaccatc gaacagatga agatgttcct
     6781 gacccgtatc ggttttaact caaaagcggt tatcaccggc gacgtcacac agatcgacct
     6841 gccgcgtaat actaaatcag gcttacgtca cgccatcgaa gtgctggccg atgtcgaaga
     6901 gatcagcttt aacttcttcc acagcgaaga cgtggttcgt caccctgtgg tggcgcgtat
     6961 cgttaacgcc tatgaagcct gggaagaagc cgaacaaaaa cgaaaagcgg cgctggcggc
     7021 agaacgcaag cgcgaagaac aggaacaaaa atgagtcagg tgatcctcga tttacaactg
     7081 gcatgtgaag ataattccgg gttaccggaa gagagccagt ttcaaacatg gctgaatgcg
     7141 gtgatcccgc agtttcagga agaatcggaa gtgactattc gcgtggtcga taccgccgaa
     7201 agccacagtc tgaatctgac ctatcgcggt aaggataagc cgaccaacgt gctctccttc
     7261 ccgtttgaag tgccgcctgg catggaaatg tcgctactgg gcgatctggt tatctgccgt
     7321 caggtggttg agaaggaagc tcaggagcaa ggcaaaccac tggaggcgca ctgggcgcat
     7381 atggtggtgc acggcagtct gcatttgtta ggttacgatc acatcgaaga tgacgaagca
     7441 gaagaaatgg aagccctcga aacagagatt atgcttgctc tgggctatga ggatccgtac
     7501 attgccgaga aagaataatt tgccaggcat ttgactggca gacgtccccg ccgttggtga
     7561 ttaacgacga cgttttgatt aactaacatg agaacccata cgacgccatg agcgacgaca
     7621 attcacacag tagtgacacg ataagcaaca agaagggatt tttctccctg ttactcagcc
     7681 aacttttcca cggtgaaccg aaaaaccgtg acgaactgct ggcgctgatc cgtgattccg
     7741 ggcagaacga ccttatcgac gaagatacgc gcgatatgct cgaaggggtg atggacatcg
     7801 cagaccaacg cgttcgcgac atcatgatcc cccgctccca gatgattacc ctgaaacgca
     7861 accagacgct ggacgaatgc cttgatgtca tcatcgagtc cgcccactca cgtttcccgg
     7921 tgattagcga agacaaagat cacattgaag ggattctgat ggcgaaagac ttgctgccgt
     7981 ttatgcgcag cgatgctgaa gccttcagca tggacaaagt gttacgtcag gcggttgtcg
     8041 ttcctgaaag taagcgcgta gaccggatgc tgaaagagtt tcgctctcag cgttaccaca
     8101 tggcgatcgt cattgacgaa ttcggtgggg tttccggtct ggtgaccatt gaagacatcc
     8161 tggaactgat tgttggtgag attgaagacg agtatgacga agaagatgat atcgacttcc
     8221 gtcagctgag tcgtcatacc tggaccgtgc gtgcactggc ttccattgaa gacttcaacg
     8281 aagcgttcgg cacccacttt agcgatgaag aggttgacac tatcggtggt ctggtgatgc
     8341 aggcatttgg gcatcttccg gcgcgtggcg aaactatcga catcgacggt taccagttca
     8401 aagtggcgat ggccgacagt cggcgtatta ttcaggttca tgtcaaaatc ccggatgact
     8461 caccccagcc gaagctggat gaataaaacc gaaactggat agataactac atggcttttg
     8521 cctcattaat tgaacgccag cgcattcgcc tgctgctggc gttattattc ggtgcctgcg
     8581 gaacgctggc cttctctcct tacgacgtct ggcctgcggc gattatttcg ttgatggggc
     8641 ttcaggcgtt gacctttaac cgccgtccac tccagtctgc cgctattggc ttttgctggg
     8701 gatttggcct ctttggcagc ggtattaact gggtctatgt cagcatcgcg acctttggcg
     8761 gaatgcctgg cccggttaac atcttcctgg tggtgctgct ggcggcgtat ttgtcgctgt
     8821 ataccggact gtttgctggc gtgctgtcgc gtctgtggcc gaaaaccacc tggctgcgcg
     8881 tggcgattgc cgcccctgct ctctggcaag tgaccgagtt tctgcgcggt tgggtactga
     8941 caggcttccc gtggttacag ttcggctata gccagattga tggcccgtta aaagggctgg
     9001 caccgataat gggcgtggaa gccattaact tcctgctgat gatggttagt ggcctgctgg
     9061 cactggcgtt ggtcaaacgc aactggcgtc cgctggtggt ggccgtcgtg ctgtttgccc
     9121 tacccttccc gctgcgttac atccaatggt ttaccccgca accggagaaa accattcagg
     9181 tttcgatggt tcagggcgat attccgcaat cactgaaatg ggatggagat cagcttctta
     9241 atacgctgaa gatttactac aacgcaacgg ctccgctgat gggcaaatca tcgttgatta
     9301 tctggccgga gtcggcgata accgatctgg aaattaatca gcaaccgttc ctcaaagcac
     9361 tggacggtga gttgcgcgat aaaggtagct cgctggtaac cgggattgtc gacgcgcgtc
     9421 tcaataagca gaaccgctac gatacctaca acaccatcat cacactgggt aaaggtgcac
     9481 catacagcta cgaatcagcc gatcgctata acaaaaacca tctagtgccg tttggcgagt
     9541 ttgtcccgct ggagtcgatt ctgcgtccgt tagcaccgtt ctttgatctg ccgatgtcgt
     9601 cgttcagccg tgggccatat atccagccgc cgctgtcggt aaatggtatt gagcttactg
     9661 cggctatttg ctacgagatc attctcggcg agcaagtgcg cgataacttc cgcccggata
     9721 ccgactatct gctgactatc tccaacgatg cgtggtttgg taagtctatt ggtccatggc
     9781 agcacttcca gatggcgcga atgcgtgcgc tggagctggc gcgcccactg ttgcgcagca
     9841 ccaacaacgg cattacggcg gtgattggcc cgcaggggga ggttcaggcg atgatcccgc
     9901 agttcacccg cgaggtgttg accactaatg tgacaccgac caccggactc acaccgtatg
     9961 cacgtaccgg caactggcca ctgtgggtgc tgacggcgct gtttggtttt gccgctgtgt
    10021 tgatgagtct gcgagcacgt aaacgttgat gtgcctgatg tatcctgcat gagcaaaacg
    10081 tactttctca ttgagctttg gggcatggtt atcatgcccc tttttcttac ggttcaggga
    10141 tacaagccac attgccatat tggggatgac gaataattgc ctgcggatca agcgagcgcg
    10201 tcctgtccat ttggatcaac ccgttcttcg ccatgacaaa tatttttcta ccatccgctg
    10261 aaaaacgaag gtgattaacg tagctggcag gataaacgcc aataatctgc ccggcattaa
    10321 tattgagcag gatgacataa ccttgcaggc catatgacac caacgctaaa tattgaccgt
    10381 ccgggctaat accctcaacg gaataaacgc tcctgctaca ccattgcgtc ataatgctgg
    10441 cttgttgatc ccctacgctt atacgtgaaa tcccctgact atcggcaaaa ataacctcgc
    10501 cgttttcagg tcgaaaacgc atcgccgata tcgttccacg cgcatcacct gcgggaaagt
    10561 cttctttctg atgcgtgatg gtattgatcc gcacgatgtt acctctgcct gctcccggaa
    10621 ccagtaatgt tctttcatcc ggggaaagtg ccataaccca ggcgcctggt ttaccgctcg
    10681 tccccgtccc gtcgtatttc tccagcggga tatccgccac atgctgataa gtcaccagat
    10741 caatcgctgg cacactatta ctggtgcgat tcatcaccca ggcttttgtg ccatcacgcg
    10801 agaggataag gctgtcagga tgtgcaccgg tggtaatcga ctgaataatc tcccctgtcg
    10861 ccacgttaat gaccgataac gcattcgctt caaatgaagt cgcataaatc aacttttcat
    10921 ccgggcttaa tgccagcgat ctgacgccct gatctttaca gccaccacaa tgtttgactg
    10981 acttttcttt cgctatgggc gctgtaaagg tatgcaggat tttcttttgt ccgaggtcga
    11041 aaacaacaat gctgttcgtg ttttcaattg cggcatacaa catattacca tcccgactga
    11101 tcgccatatc atggatatcg gcaatctcag cgagcaaatc accactggcc tgttcacgac
    11161 ttaccggagc ggataatgtt tccctgaggg cgttgcgttc ctccgctaac tgctgctttt
    11221 tttgccgtcg ctcttgttca atctcctgtg gataaaacgc ttcacgcagg ctttgcctga
    11281 cctgtaaaac tgactcatcc atctgctgct gcgcaagcca gatagccccg gtcagaagaa
    11341 gactgcccgc aacaataatc ccgccttccg taacgagtcg gttttgccca gataaacgcg
    11401 cgctcagcca acccaacgcg ccacccgcaa tcaggccgcc aatgtggcaa gcgttatcta
    11461 ttcctgtttg ccgggcaccg tacaacagtg tcagcgccac catacccaac agaggaaata
    11521 ctcgccgctg gtttttatgg tatgtgcccg tacctgaagc aaactgtgtt gctatcgctg
    11581 caccagctat tcccagaatc gccccggatg ccccaacgct gatgactatc tgctcacttt
    11641 cgcgtaacgc ataacaggcg ctgatgagcc caccaaagac gccggagaat aaccaggtaa
    11701 tcaggagtcg ccatttcccc agaatctgtt ccgccacgcc tcccacgacc aggaatgcaa
    11761 acatattcat cagcagatga gaaacactac tgtgcagaaa gatactgctc aataagcgcc
    11821 agggttcacc cgacaaagta agaggcgcaa cgtttgcgcc gagatggata agtgcggatt
    11881 gatcgacctc ttcataactc atccattgtg cgagataaca aaatatccca atacagagtg
    11941 caaaaaggat aagggttatt gcgggtaatt gaacatttaa cggcttaacc gaagatgcag
    12001 acataaggtg tttcttcctt gaaaagacag gtaaaaagca tttttcccgt gtgcagttat
    12061 catcccggca aacgtcccat gccgaaaaga taattctgtc cgaaatatga ctttttcagc
    12121 ctaaaattac aatcgtaatt aattcgcccc taagtatttt ttggcatgta agttgcttta
    12181 tttactgtgt aagcgatgaa atcagccgcg acgcaaccag gttgcaccac cacaaatcag
    12241 cggtagcacc aaaaaggtgc ggtttaagaa ttttgcttcg gaatggtgcg gcgcatctcg
    12301 caaaaacaaa caataaaaca gcaatatctt tacatttagc caaactaaat gttacctaac
    12361 aggcacaaca ctgcacaata aagttgcaga cgataacaac acaaacactc acaacgggta
    12421 tccatgcgtt cttaacgcag aagataaagg agttggatat gcaattacgt aaacctgcca
    12481 cagcaatcct cgccctggcg ctttccgcag gactggcaca ggcagatgac gccgccccgg
    12541 cagcaggcag tacgctggac aaaatcgcca aaaacggtgt gattgtcgtc ggtcaccgtg
    12601 aatcttcagt acctttctct tattacgaca atcagcaaaa agtggtgggt tactcgcagg
    12661 attactccaa cgccattgtt gaagcagtga aaaagaaact caacaaaccg gacttgcagg
    12721 taaaactgat tccgattacc tcacaaaacc gtattccact gctgcaaaac ggcactttcg
    12781 attttgaatg tggttctacc accaacaacg tcgaacgcca aaaacaggcg gctttctctg
    12841 atactatttt cgtggtcggt acgcgcctgc tgaccaaaaa gggcggcgat atcaaagatt
    12901 ttgccgacct gaaagacaaa gccgtagtcg tcacttccgg cactacctct gaagttttgc
    12961 tcaacaaact gaatgaagag caaaaaatga atatgcgcat catcagcgcc aaagatcaca
    13021 gtgactcttt ccgcaccctg gaaagcggtc gtgccgttgc ctttatgatg gatgatgccc
    13081 tgctggccgg tgaacgtgcg aaagcgaaga aaccagacaa ctgggaaatc gtcggcaagc
    13141 cgcagtctca ggaagcctac ggttgcatgt tgcgtaaaga cgatccgcag ttcaaaaagc
    13201 tgatggatga caccaccgcg caggtgcaga cctccggtga agcggaaaaa tggtttgata
    13261 aatggttcaa aaatcccatt ccgccgaaaa acctgaacat gaatttcgaa ctgtcagacg
    13321 aaatgaaagc actgttcaaa gaaccgaatg acaaggcact gaactaatta caagaaccag
    13381 gggcggaaat tccagccctc tcgattgtta cgtagcacgg acagactata cgcctgatgg
    13441 tcgttcccca tcgggcctga aaaccgcaat acgctgggta acaatcttcg agggtagcag
    13501 ttaacgctgc taccctcttt tttctggagt agatttatgt ctatagactg gaactggggt
    13561 atttttttac aacaagcccc gttcggcaac accacctatc ttggttggat ctggagtggt
    13621 tttcaggtga cgatcgcttt gtcgatctgc gcctggatta tcgctttcct cgtcggttca
    13681 tttttcggca ttttacgtac cgttccaaac cgttttctct ccggtctggg tacgttgtat
    13741 gtcgaactgt tccgcaacgt gccgttgatt gtgcaattct ttacctggta tctggtgatc
    13801 ccggagctgt tgccggagaa aatcggcatg tggtttaagg ccgagctgga tcccaatatt
    13861 cagtttttcc tttcatccat gctctgcctg gggctgttta ccgccgcccg tgtttgcgaa
    13921 caggtacgcg ccgccattca gtcgttgccg cgtgggcaaa aaaatgccgc gctggcgatg
    13981 ggcctgacgc taccgcaggc ttatcgatac gtactgctgc ctaatgctta tcgcgttatc
    14041 gtcccgccga tgacttccga gatgatgaac ctggtgaaaa actccgccat cgcctcgact
    14101 attggtctgg tggatatggc ggcgcaggcg ggtaaattgc ttgattactc agcccacgcc
    14161 tgggagtcgt ttaccgctat tacgctggcg tatgtgttga ttaacgcggt tatcatgctg
    14221 gtaatgacgc tggttgaacg taaagttcgc ctgcctggca acatgggggg caaataatgt
    14281 acgagtttga ctggagttcc attgtccctt ccctgccata tctgctcgac gggctggtga
    14341 tcacgctgaa aatcaccgtc acggcagtcg tgattggtat tttgtggggc accatgctgg
    14401 cggtgatgcg cttgtccagc tttgcgcccg tagcgtggtt tgccaaagcc tacgttaacg
    14461 tattccgctc gattccttta gttatggttt tgctgtggtt ttacctgatc gtgccaggtt
    14521 ttctgcaaaa cgtgctggga ttatcaccaa aaaatgatat ccgtttaatt tcggcgatgg
    14581 tggcgttttc gatgtttgaa gcggcctact attcagagat tatccgtgct ggtattcaaa
    14641 gtatttctcg tggtcagtcg agcgccgcgc tggcgttggg aatgactcac tggcagtcga
    14701 tgaaactgat tattctgccg caggcgttcc gcgcgatggt gccgcttctg ctcactcagg
    14761 gcatcgtact gttccaggat acctcactgg tgtatgtgtt atctctggcc gatttcttcc
    14821 gtaccgcctc aaccattggt gagcgtgatg gtacgcaggt tgagatgatc ctgtttgccg
    14881 gatttgttta tttcgttatt agtcttagcg cgtcgttgtt ggtcagctac ttgaaaagaa
    14941 ggacagcata atgattaccc tgaaaaatgt ttcaaaatgg tatggtcact ttcaggtgct
    15001 gaccgactgc tcaaccgaag tgaaaaaagg cgaagtggtg gtggtttgcg gcccgtctgg
    15061 ttccggcaaa tcaacgctga ttaaaaccgt caacggcctc gaaccagtgc agcaaggtga
    15121 aatcaccgtc gatggtatcg tggttaatga caagaaaacc gatctggcaa agctgcgttc
    15181 ccgcgtcggg atggtattcc agcatttcga gctgttccct catctgtcga ttatcgaaaa
    15241 cctgaccctg gcgcaggtga aagtgcttaa acgcgataaa gcgcccgctc gtgaaaaagc
    15301 attgaaattg ctggagcgcg tgggactttc cgcacatgcc aataaatttc cggcgcagct
    15361 ttccggcggt cagcagcagc gtgtggcaat cgctcgcgca ttgtgtatgg atcctattgc
    15421 gatgctgttc gacgaaccga catcggcgct ggatccggag atgatcaacg aagtgctgga
    15481 cgtgatggtg gaactggcga acgaaggaat gaccatgatg gtggtgaccc acgaaatggg
    15541 ctttgcccgc aaagtggcga atcgggtgat ctttatggac gagggtaaaa ttgtcgaaga
    15601 ctcgccgaaa gacgctttct tcgatgatcc gaaatcggac cgcgcgaaag acttcctcgc
    15661 gaaaatcctg cattaatcac tctggcgcgc atacccaagt gcgcgccatc tcacgcttta
    15721 tcgtttcgcc cctcgtatct gttacatcct ggcgttaccc ttgtcgcaaa gaagcacaca
    15781 acaaggagca acaatggcac tgccaattct gttagattgc gacccaggtc atgacgacgc
    15841 tatcgcaata gttctcgccc tcgcctcacc agagcttgat gtcaaagcaa ttacgtcttc
    15901 cgccggaaac cagacaccag aaaaaacctt acgcaatgtt ctgcgtatgc tgaccttgct
    15961 taatcgcacc gatattccgg tagcaggcgg cgcggtaaaa ccgttaatgc gtgagttgat
    16021 tatcgcggac aatgtgcacg gcgaaagcgg tctggatggc ccggcattac cggaaccgac
    16081 gttcgcaccg caaaactgta cggcggtaga gctgatggcg aaaacgctgc gcgaaagtgc
    16141 ggaacctgtc acgattgtgt ctaccggacc gcaaactaac gttgccttgc tgctcaatag
    16201 ccatccggaa ctgcatagca aaattgcccg tatcgtgatt atgggcggcg caatggggct
    16261 tggtaactgg acgcctgcgg ctgaatttaa tatttacgtt gacccggaag cggcagaaat
    16321 tgtcttccag tcagggatcc cagtggtgat ggccggtctg gatgttactc acaaagcaca
    16381 aatccacgtt gaagacaccg agcgtttccg cgcgattggc aaccctgttt caaccattgt
    16441 tgccgaactg ctggatttct tcctcgaata tcataaagac gaaaaatggg gctttgtcgg
    16501 tgcaccgctg catgacccat gcaccatcgc ctggctgttg aaacccgagt tgtttaccac
    16561 tgttgagcgc tgggttggcg tggaaacaca ggggaaatat agccagggta tgacggttgt
    16621 tgattattat tatctgactg gcaataaacc gaatgccact gtaatggtcg atgttgatcg
    16681 tcagggcttt gttgatttac tggctgatcg tctgaaattt tacgcttaac acacgtcatt
    16741 gattaagatc accacgcgtt acctgggtgg tcttaatctg tttatctgtt tatctctttt
    16801 tctttcaaga tataaatgca atggataatg cagaactcgc cattggtatc gatctcggca
    16861 ctaccaatag tttaattgcc gtctggaaag acggtgccgc gcaattaatt ccaaataagt
    16921 tcggtgaata tttaacacca tccataatta gcatggatga aaataatcat attttagtcg
    16981 gaaaaccggc tgtatcacgg cgtacttcgc atccggataa aacggcagcg ttatttaaac
    17041 gtgcaatggg cagtaatacc aactggcggt taggcagcga cacatttaac gcgccagaac
    17101 tgtcctcttt ggtattacgc tcattaaaag aagatgccga agaatttctg caacgtccga
    17161 ttaaagatgt ggtgatctcc gttccggctt atttcagcga tgaacaacgc aagcataccc
    17221 gtttagcagc ggagttagcc gggttaaatg cggtacgctt aattaatgaa cccacagcag
    17281 ctgcgatggc atatggcctg catacgcaac aaaatacccg ctcgctggtt tttgatctcg
    17341 gtggcggcac gtttgacgtc acggtgctcg aatacgccac gccggtgatt gaagttcacg
    17401 cctccgctgg cgataacttc ctcggtggcg aagattttac ccatatgttg gtcgatgacg
    17461 ttttaaagcg cgcggatgtc gccaggacca cgcttaacga gagtgaactg gcagccttgt
    17521 acgcctgtgt ggaagcggca aaatgtagca atcaatcgcc attgcacatt cgctggcagt
    17581 accaggatga aacgcgggag tgcgaatttt acgagaacga actggaagat ttgtggttgc
    17641 cgctgctcaa tcgcttgcga gtgccgattg aacaggcgtt gcgcgatgca cgtctgaagc
    17701 cgagtcaaat cgacagtctg gtgctggttg gcggcgcgtc acaaatgccg ctggtgcagc
    17761 gaatcgccgt gcgtctgttt ggcaaattac cgtatcaaag ttacgacccg agcaccattg
    17821 tcgcgctggg cgcagcaatc caggccgcct gccgcttacg cagtgaagat attgaagagg
    17881 tgatcctcac tgatatttgc ccttactcgt tgggcgttga agttaaccgc cagggcgttt
    17941 ccggcatttt ctcgccaatt attgaacgaa acaccacagt acccgtgtcg cgtgtagaaa
    18001 cttactcgac catgcacccg gaacaggatt ctattacggt taacgtctat cagggagaaa
    18061 accacaaagt taaaaataac attctggttg aatccttcga tgtgccgttg aagaaaaccg
    18121 gggcttatca gtcgattgat attcgcttta gttatgatat caacggcttg cttgaagttg
    18181 acgtgcttct ggaagatggc agcgttaagt ccagagtcat taatcatagc ccggtaacat
    18241 tgagcgcgca gcagattgaa gagagtcgga cgcggttatc cgcattgaaa atttatccgc
    18301 gcgatatgct aatcaaccgc acctttaagg ccaaactgga agagttgtgg gcgcgggcgc
    18361 tgggtgacga gcgagaagag attggccggg tgatcaccga ttttgatgcg gcgttgcagt
    18421 caaacgatat ggcccgcgtc gatgaagttc gtcgacgggc gagcgattat ttggctattg
    18481 agatccctta aacacggctg ccggatgcgg cataaacgct gcatccggca tcggcgcaaa
    18541 aaacacatcc tcagctaaac aacatcgcga tttttccgag cgcataacct atcatcagca
    18601 ccgttaagct gattttgata atactggtaa tgctctgtgg tttttcatcc tcatcatttt
    18661 tttcttcatg gttttcaggt gttggctcat cgacatcttc atcctccggc tcgagtttca
    18721 ctttcgtcaa cagcatctgg gtgataatgg cctctaatgg atgagcatct ccaccttcca
    18781 gccttgtatt aagacgggcc agcatctctt gtttcgggaa cgcttccagc acaacctgtt
    18841 ccgctacccg atacaaatcc agtaacggtg aatcttgctc tgtctcgcta ccgactctcg
    18901 atttccacca cgcccatgct ttgcgcttga agaataacga ctcaataaac gattgaccta
    18961 acggcgataa ttttccgcta tgtaaaatct ccgcccataa cagccgcgtc cgggcgctct
    19021 caccaggaac atatttcaac ggtttacctt gcgcatctac catgcttcgc gcttcgataa
    19081 ccatcactaa taacgcgaaa taatccgggc aatgacgctt gctccactgc aacaacaaat
    19141 tatcagcttg tgtagatggg taactttccc agtacgcgca gagatcggca agcaggcttt
    19201 ccccctcgcc gcagtacaaa cgttgtgcac actggtaata ccgggcatct tcgctttctg
    19261 gttcttccgc ttgccactgt ttggctacag gaatgagttc cgccatacct gtttgcaccg
    19321 agctgtacca gcgcaacagt ttgcgatgta actttgcatc atcaggaatg aaccacggtc
    19381 cttccatcgc caaaaacgca gtgacatagt caggatggta acggcagcaa cgttccagcg
    19441 cgaagtaata ttcaacagtc tggttctgta acgcgacggg atattccaga aggctaagga
    19501 aatcgaaaca atcaccagcc tgaatggcct caagaaaagc ctccctttct tcctcatcca
    19561 ttccttcgct gtcattaaac gaatgccagt tcaaacgctc tgccaacaaa gaagcacaat
    19621 ttaatgaaag atatcgcgct tctatagcga ttgcacacag cggccagcgt aattgatcga
    19681 catcctccat gttccaggta tttaattgct gaataaattt ctgccaggcg gagggctgaa
    19741 aacgatcact ttctgaatcc agtaatgtcc tgaatgcacg tagtatttca tgttcagcgg
    19801 tggcatcttt ttcttcatca tcggcttcct cgacaggatt taccgccagt cgcagcgcct
    19861 cttcataggc ctggcgtaat gctttaaacc cctgcggatc ggtttcggga tgacacaacg
    19921 gtaagcgagc aagataagcc tggcggataa tgtctatttg cgtcgtgctt tcaatttcga
    19981 ggatttgcca acatgttttc ataggtcgct cacatcctta agcgttaccg ggcattcagg
    20041 ctggacagtt aatggttgcc agggaagcgt ggcatagaaa cggtcgcttc ccgctgccag
    20101 ctcttcgaac atccgactcc cggcgctatc gtatttgtac agggttagcg cctcgcgtag
    20161 cgaagcgttg tcttcgcact gagaagattg ccagtagaga cgccctagtg aataggcaga
    20221 aaaatagtgc atccagctgt gatagtaatg gtgagctcgt gcatagacgc gcgattgcag
    20281 ccataaactc tcttcttccg tcagccagtt gttgagcaca ccgacccgtg acagaaaccc
    20341 cattcgaaca taatcccagg ctttgattcc cccttctcca cagcacatag cggtttgggc
    20401 aacgaattgg gtataaatca accctctctc cgagccacca gcaattaatg ctttcctctc
    20461 ttcaggggaa gagcgttgcc atgtcagata taagccagct aaatcattgg catgaccatc
    20521 atcagtcatc tgataaatag tctgaattaa atcatcacga ttatttattc cccaggatga
    20581 tgagagatcg aattctttta tcccagtata catttttggc gagtcatgcg cgggtttgca
    20641 actctgatta agtatttcca tgggcgcaga aagcgcaaaa agaagatatt gttcttcctt
    20701 attcattttt taataatccc ttaagtgcat taactgacta cttttttatt attgatgagt
    20761 ataaacttga cgctttcaaa ataaaaagaa aatcgaagca ttcacacatg aataaaaaat
    20821 caatgtatat attcgcaatt tttatagttg cagcaattac ctgtattagc caacccaaga
    20881 aaacgacgtt gcgtgataaa gccatggtga attatgcctt tgattattta agatcaccag
    20941 gcagtctacc gttaaccacg gcagccacgg agctttccgc gattcatggt cactcaacat
    21001 cgcaatatcg ccttggcgaa ttttatcttc atggtagcga cggtaaacca ctggattata
    21061 cacaggcgag atactggtat gagcaatcag cagaacagga aaatccgcgc gcacaaagca
    21121 aactggggtg gatctacctg aaaggcctgg gagtcaaacc cgatacccgt aaagcaattc
    21181 tctggtataa ggaagcagct gaacaagggt atgctcatgc tcaatatact ttgggtttga
    21241 tctacagaaa tggcactggt attaatgtta accattatga atctcaaaaa tggttaaaac
    21301 tggccgccaa acaacattac aaaaatgcgg aaagattact tgccggactt cccgcacatt
    21361 aacgtcgact gtcacccgaa gagatggagt atttttccta tcaaaccggc aaagataaaa
    21421 atgtagaaaa taattttgat gacgcccaga cagcccagcc cgccatcgtc aacgggcatt
    21481 ggctgcggtt gcggctccgg ctcgacggtt tcctgaccgg taaaattcgc cgcctgaatt
    21541 aataatcgac gaataattaa tgcttccagt ggacaacgat cttctgcatc aaggcggctc
    21601 acaatcggca ttaatgattc ttctgtcggt tggctggcct gcactaattg tttggcaact
    21661 aaatataatg gccataaggg atgttctgta tcacgcgtga gtgagatccg cggcttacgt
    21721 cctttgtgca aaaccatctc gaccagtgaa cgccccagcg cagagagttt ttcatcgctg
    21781 agtatttcgt ataaatgaaa acgcgtacgg gcgctgtcgc cggggacata aagtagctgt
    21841 ttaccctgat cgttaaccag ttcacgagct tcaatcatca tcaccactaa cgggtaataa
    21901 tcgacccgat gctgacggca ccattgcaat attaaagcat ccgcctgggt ggagggatac
    21961 tcgcgccagt agtcacaaag ttctgccagc aagctttcgc cttctccaca atagacgcgt
    22021 tgcgcgtatt cataataacg cgcatcttca ttatcaggtt cagcttcccg ccacgaccgg
    22081 gcgtaatcca gaagttcggg gatatccagg cgacctgcgc tataccagcg gagtaaattt
    22141 ctgtgcagtt ttgcatcatc ggggatgatc actgcgcgat gttgcgctaa atacaatgtc
    22201 agccattctg gatgcgatga ccaaatccga gcgaacattt ggtaaaaatc gatggtgtca
    22261 ttttgcacgg caacgggcag atgcagaatg gtctggaagt taaaaacatt ccccttcgca
    22321 atggcatata aaaaggattc cagttcttct tcgtctattt ccccgacgtt ttcctcctgc
    22381 aaccaccgca atctttctgc taataacacc acgcactcga aggataaatg ggcagtgttc
    22441 atggcgattg tgcacagcga ccagcgtaat tcatcaatct cttccatcga gcaataattt
    22501 aattgctgaa tgaatcgctg ccaggcggag ggcagaaaac gttcgctatc agaggcaagt
    22561 aacgcgcgaa aggcgagcag aatttcatgt tctgctacct catattcttc tggttgccaa
    22621 acagatttag caggcgactg cgcaatccgg agcgcctcct catacgcctg acgaagttgt
    22681 ttaaaaccct gcggatcggt ttctggatga aaggacagta acagcgccag ataagcgcgg
    22741 cggataatat cgacgtcagt cgtttcctct atttcgagga tcttccagca atttttcata
    22801 ggtcgctcat atccttaagc gtttccgggc atgtggggta gtcagccagt ggttgccagg
    22861 acaaggcagg gtaatacgca tcatcactgg caaataattg ataaaacata tcattgccgg
    22921 aatcgtcgta ttctttacga gctaagaatt cgcgtaataa aggcagatga tcatcttcgg
    22981 gagactgcca atattgccga ccaaaggtat aaccggcaaa gtattgccgc cagttactat
    23041 aataacgtag agcccgtaga tgaatacgcg actggatcca tagactttct tcttcactca
    23101 accagttatt aagtacgccc attcgactta aaaaacccat ccggacataa tcccatgctt
    23161 taattcctcc ctcaccacag cattcggctg tgctggcgac aaattgcgca taagcctgac
    23221 cttgctcatt tagttcagca agatagtcac gccattcaca tggcgaataa cgaaaccagc
    23281 ggtgataaaa ccctgccaac cgagcggcat gaccgttatc gatcattcgg tgaattgtgc
    23341 gcagcaggtc atcacggtca tcaattcccc atgaggaaga aaggtcaaaa aagcatgtac
    23401 ctaaatacat tttgggggat gaatgcgaag gtaaacagca ttcattgcgt atttccatcg
    23461 gagtggaaag tgcaaacaat attttttgcg accccatgtc catataattt atccttaaac
    23521 attcatttaa tgaatgtaaa ctacatttcg agactcagtt atttatactt ttctgacgca
    23581 acataatacg cataaaataa ggcttacgca aaaatggaac acataaaaat ataatatgtt
    23641 ttatctacaa ccataaggaa atgataatgg tttttacgtc aagttgctgc gataatttat
    23701 caatagatga gattatcgaa cgtgctgaaa aaggcgattg cgaggctcaa tatattgttg
    23761 ggttttatta taatcgcgat agcgcaattg actctccgga cgacgaaaag gccttttact
    23821 ggctgaagct ggccgctgag caaggtcatt gtgaagcaca gtattcctta gggcaaaagt
    23881 ataccgagga taaaagccgt cataaagata atgagcaagc catcttctgg ctgaaaaaag
    23941 ctgccctaca aggccatact ttcgcttcca acgcccttgg ctggacactg gatcgtggag
    24001 aagcccccaa ttataaagaa gcggttgtct ggtatcagat agccgcggag agcggaatgt
    24061 cttatgcgca aaataatctt gggtggatgt acagaaatgg caacggagtc gcaaaagact
    24121 atgcgctggc atttttctgg tacaaacaag ctgcattaca aggccatagt gacgcgcaaa
    24181 acaatctggc cgatctttat gaagacggaa aaggcgttgc tcaaaacaag acactcgccg
    24241 cattctggta tttgaaaagc gcacagcagg gtaatcggca cgcccagttt caaattgcat
    24301 gggattataa cgctggcgaa ggggtggacc aggactataa gcaagcgatg tactggtatc
    24361 tgaaggctgc cgctcaggaa agcgtcggcg cttacgtcaa catcggttat atgtataaac
    24421 acggacaagg cgttgagaaa gattatcagg ctgcctttga atggtttacg aaagccgctg
    24481 aatgcaatga cgccactgcc tggtataacc tggccattat gtatcactac ggagaaggaa
    24541 gacctgtcga tctccgacag gctctcgacc tgtatcgtaa agttcagtca tccggaacca
    24601 gagatgtcag tcaggaaatt cgtgagactg aagatttact gtagagataa aaacaagggc
    24661 cggggaattt cattcggccc atattctctt tattgctgtg cgaaatcaga ctattacggc
    24721 tcaaacgggc gtctctggaa ctggtcatga ccacatttcg ggcatagcgt cagcacttcc
    24781 ggtgtgtaga tcgggagatg gaagtgacat ttctcgcaga ccagatttcc cagcccgacc
    24841 acttctccgc tgtgataaac cccatgatga ttgaggtcct ggaaaacttc gcgccattca
    24901 agctgcgttt tatcggtgat atctgccagc tcctgccata agctttcttt aatcacccgc
    24961 ataaagacgc tgtcagattc ttctttcagg ctctcttcat agctcatggc gaactcttcc
    25021 aggtcacgtc ttacagctcg cgtcagctca tcgacctcgg ttcgcgttaa ctcccctgtt
    25081 tttattacgc gctcgcgcgc ctgttccacc agtgcgtcga tatcacgttc gccattgcgc
    25141 aggcgttcgc tcagtgacgc gaccagttca cggtaatatt gagcaacctt gttcatcgtt
    25201 tcatctcccg ggtcgttaac tgactataat aatagacgtt ctttgttcct tgctttgcta
    25261 atacgcccac aaactctgta aattaatgta tgaataatcc gggcatcatt tcaacgtcga
    25321 gtgcgcgaaa ggctgttttg acgcggacgt ttgggctatg ctatgcggat ctgaaaaacc
    25381 acctcaacgc tacatttgta gccgtattga aaacaggacc actggctgcc atgcaagagc
    25441 aataccgccc ggaagagata gaatccaaag tacagcttca ttgggatgag aagcgcacat
    25501 ttgaagtaac cgaagacgag agcaaagaga agtattactg cctgtctatg cttccctatc
    25561 cttctggtcg actacacatg ggccacgtac gtaactacac catcggtgac gtgatcgccc
    25621 gctaccagcg tatgctgggc aaaaacgtcc tgcagccgat cggctgggac gcgtttggtc
    25681 tgcctgcgga aggcgcggcg gtgaaaaaca acaccgctcc ggcaccgtgg acgtacgaca
    25741 acatcgcgta tatgaaaaac cagctcaaaa tgctgggctt tggttatgac tggagccgcg
    25801 agctggcaac ctgtacgccg gaatactacc gttgggaaca gaaattcttc accgagctgt
    25861 ataaaaaagg cctggtatat aagaagactt ctgcggtcaa ctggtgcccg aacgaccaga
    25921 ccgtactggc gaacgaacaa gttatcgacg gctgctgctg gcgctgcgat accaaagttg
    25981 aacgtaaaga gatcccgcag tggtttatca aaatcactgc ttacgctgac gagctgctca
    26041 acgatctgga taaactggat cactggccag acaccgttaa aaccatgcag cgtaactgga
    26101 tcggtcgttc cgaaggcgtg gagatcacct tcaacgttaa cgactatgac aacatgctga
    26161 ccgtttacac tacccgcccg gacaccttta tgggttgtac ctacctggcg gtagctgcgg
    26221 gtcatccgct ggcgcagaaa gcggcggaaa ataatcctga actggcggcc tttattgacg
    26281 aatgccgtaa caccaaagtt gccgaagctg aaatggcgac gatggagaaa aaaggcgtcg
    26341 atactggctt taaagctgtt cacccattaa cgggcgaaga aattcccgtt tgggcagcaa
    26401 acttcgtatt gatggagtac ggcacgggcg cagttatggc ggttccgggt cacgaccagc
    26461 gcgactacga gtttgcctct aaatacggcc tgaacatcaa accagttatc ctggctgctg
    26521 acggctctga gccagatctt tctcagcaag ccctgactga aaaaggcgtg ctgttcaact
    26581 ctggcgaatt caacggtctt gaccatgaag cggccttcaa cgccatcgcc gataaactga
    26641 ctgcgatggg cgttggcgag cgtaaagtga actaccgcct gcgcgactgg ggtgtttctc
    26701 gtcagcgtta ctggggcgcg ccgattccga tggtgacgct ggaagacggt accgtaatgc
    26761 cgaccccgga cgaccagctg ccggtgatcc tgccggaaga tgtggtgatg gacggcatta
    26821 ccagcccgat taaagcagat ccggagtggg caaaaactac cgttaacggt atgccagcgc
    26881 tgcgtgaaac cgacactttc gacaccttta tggagtcctc ctggtactat gcgcgctaca
    26941 cttgcccaga gtacaaagaa ggtatgctgg attccgaagc ggctaactac tggctgccgg
    27001 tggatatcta cattggtggt atcgaacacg ccattatgca cctgctctac ttccgcttct
    27061 tccacaaact gatgcgtgat gcaggcatgg tgaactctga cgaaccagcg aaacagttgc
    27121 tgtgtcaggg tatggtgctg gcagatgcct tctactatgt tggcgaaaac ggcgaacgta
    27181 actgggtttc cccggttgat gctatcgttg aacgtgacga gaaaggccgt atcgtgaaag
    27241 cgaaagatgc ggcaggccat gaactggttt ataccggcat gagcaaaatg tccaagtcga
    27301 agaacaacgg tatcgacccg caggtgatgg ttgaacgtta cggcgcggac accgttcgtc
    27361 tgtttatgat gtttgcttct ccggctgata tgactctcga atggcaggaa tccggtgtgg
    27421 aaggggctaa ccgcttcctg aaacgtgtct ggaaactggt ttacgagcac acagcaaaag
    27481 gtgatgttgc ggcactgaac gttgatgcgc tgactgaaga tcagaaagcg ctgcgtcgcg
    27541 atgtgcataa aacgatcgct aaagtgaccg atgatatcgg ccgtcgtcag accttcaaca
    27601 ccgcaattgc ggcgattatg gagctgatga acaaactggc gaaagcacca accgatggcg
    27661 agcaggatcg cgctctgatg caggaagcgc tgctggccgt tgtccgtatg cttaacccgt
    27721 tcaccccgca catctgcttc acgctgtggc aggaactgaa aggcgaaggc gatatcgaca
    27781 acgcgccgtg gccggttgct gacgaaaaag cgatggtgga agactccacg ctggtcgtgg
    27841 tgcaggttaa cggtaaagtc cgtgccaaaa tcaccgttct ggtggacgca acggaagaac
    27901 aggttcgcga acgtgctggc caggaacatc tggtagcaaa atatcttgat ggcgttaccg
    27961 tgcgtaaagt gatttacgta ccaggtaaac tcctcaatct ggtcgttggc taagcgcggg
    28021 aggaagcgtg cgatatctgg caacattgtt gttatctctg gcggtgttaa tcaccgccgg
    28081 gtgtggctgg catctgcgtg ataccacgca ggttccttcc actatgaagg tcatgatcct
    28141 ggactcaggc gatccgaacg ggccattaag ccgtgcagtg cgtaaccagt tacgtctgaa
    28201 tggtgtcgag ttgcttgata aagaaaccac gcgtaaggac gttccatccc tgcgtttggg
    28261 taaagtgagc atcgcgaaag ataccgcatc ggtattccgt aacggtcaaa cagcagagta
    28321 tcagatgatc atgacggtga atgcgaccgt gttgatcccc ggccgtgata tctacccgat
    28381 tagcgccaaa gtcttccgtt cgttctttga taacccgcaa atggcgttag cgaaagataa
    28441 cgaacaagac atgatcgtaa aagagatgta cgaccgtgct gccgaacagc tgattcgtaa
    28501 gctgccaagc atccgtgctg cggatattcg ttccgacgaa gaacagacgt cgaccacaac
    28561 ggatactccg gcaacgcctg cacgcgtctc caccacgctg ggtaactgat gattcggttg
    28621 tacccggaac aactccgcgc gcagctcaat gaagggctgc gcgcggcgta tcttttactt
    28681 ggtaacgatc ctctgttatt gcaggaaagc caggacgctg ttcgtcaggt agctgcggca
    28741 caaggattcg aagaacacca cactttttcc attgatccca acactgactg gaatgcgatc
    28801 ttttcgttat gccaggctat gagtctgttt gccagtcgac aaacgctatt gctgttgtta
    28861 ccagaaaacg gaccgaatgc ggcgatcaat gaacaacttc tcacactcac cggacttctg
    28921 catgacgacc tgctgttgat cgtccgcggt aataaattaa gcaaagcgca agaaaatgcc
    28981 gcctggttta ctgctcttgc gaatcgcagc gtgcaggtga cctgtcagac accggagcag
    29041 gctcagcttc cccgctgggt tactgcgcgc gcaaaacagc tcaacttaga actggatgac
    29101 gcggcaaatc aggtgctctg ctactgttat gaaggtaacc tgctggcgct ggctcaggca
    29161 ctggagcgtt tatcgctgct ctggccagac ggcaaattga cattaccgcg cgttgaacag
    29221 gcggtgaatg atgccgcgca tttcacccct tttcattggg ttgatgcttt gttgatggga
    29281 aaaagtaagc gcgcattgca tattcttcag caactgcgtc tggaaggcag cgaaccggtt
    29341 attttgttgc gcacattaca acgtgaactg ttgttactgg ttaacctgaa acgccagtct
    29401 gcccatacgc cactgcgtgc gttgtttgat aagcatcggg tatggcagaa ccgccggggc
    29461 atgatgggcg aggcgttaaa tcgcttaagt cagacgcagt tacgtcaggc cgtgcaactc
    29521 ctgacacgaa cggaactcac cctcaaacaa gattacggtc agtcagtgtg ggcagagctg
    29581 aaagggttat ctcttctgtt gtgccataaa cccctggcgg acgtatttat cgacggttga
    29641 tatgaaatct ttacaggctc tgtttggcgg cacctttgat ccggtgcact atggtcatct
    29701 aaaacccgtg gaaacgctgg cgaatttgat tggtctgacg cgggtcacaa tcatccctaa
    29761 taatgttcct ccgcatcgtc cccagccgga agcgaacagc gtgcagcgta aacacatgct
    29821 tgaactggcg attgccgaca agccattatt tactcttgat gaacgcgagc taaagcgcaa
    29881 tgccccctct tacactgcgc aaacactgaa agagtggcgg caggaacaag gaccggacgt
    29941 gccgctggcg tttattattg gtcaggattc actgctgacc tttccgacct ggtacgaata
    30001 cgaaacgata ctcgacaatg cacatttgat cgtctgtcgg cgtccaggtt acccacttga
    30061 aatggcgcaa ccgcaatacc agcaatggct ggaagatcat ttgacacata acccggaaga
    30121 tcttcacctt cagcctgccg gtaaaattta tctggctgaa acgccgtggt ttaacatctc
    30181 ggcgaccatc atccgcgaac gtttgcaaaa cggtgaatcg tgtgaggatt tattgccgga
    30241 accggtactg acttacatta accaacaagg cttgtatcgc tgatacctgt cgttacggag
    30301 ggcatgatgc gactgtggtt aattcgtcat ggtgaaacgc aagcgaatat cgatggtctt
    30361 tacagcggtc atgcgcccac ccccctgacc gcgcgcggta ttgagcaagc gcaaaatctg
    30421 catacgctgc tacatggtgt ttcctttgat ctggttttat gcagtgaact ggaacgggca
    30481 cagcataccg cacgactggt tctcagtgac cgccagctcc ccgtgcaaat catccctgaa
    30541 ctcaacgaaa tgttttttgg cgactgggag atgcgacatc atcgcgacct catgcaagaa
    30601 gatgccgaaa actatagcgc gtggtgcaat gactggcagc atgcaatccc cacgaacggt
    30661 gaaggatttc aggcattttc gcaacgtgtg gaacgcttta tcgcaaggct tagtgaattt
    30721 cagcactatc agaatatttt agtcgtcagc catcagggtg tactgagtct gttaatcgcc
    30781 cgtttaattg gcatgcctgc cgaggctatg tggcattttc gcgttgacta ggggtgttgg
    30841 agcgccattg atatcaacca aaaattcgcg acgctacgcg tcctcaatag ccgtgccatc
    30901 ggggtcgaga atgcatgact tttctgtttt tttacgggta agccgcaacg accattgaca
    30961 gacccgggca ggctgatatt ctccgcagcc agactttttt cgccagacac gactttgtag
    31021 aaattgtttt acaaaaatgg cgatgcaatc tgcggcgcgg ggtgggatga tagcccactt
    31081 tcgaaagccg attcggcgac aattgtcccg aaatcgcctc tggttcaggt atactgacag
    31141 accattttta tctatttgat tcacccaggg ggaaaacttg cagggtaaag cactccagga
    31201 ttttgttatc gacaaaattg atgacctcaa aggtcaggac atcatcgcct tagacgttca
    31261 aggcaaatcc agcatcaccg actgcatgat catctgtacg ggtacgtcca gccgtcatgt
    31321 tatgtccatt gctgaccacg ttgtgcagga gtctcgcgca gcgggcctgt taccgctcgg
    31381 cgtagaaggt gaaaacagcg ccgactggat tgtcgtggat ttgggcgatg tgattgtcca
    31441 tgtcatgcag gaagagagcc gtcgcctgta tgaactggaa aaactctgga gttaatgcgt
    31501 gaagctgcaa cttgtcgccg tgggaacgaa aatgccggac tgggtacaaa ccggttttac
    31561 cgagtacctg cgtcgttttc cgaaagatat gcccttcgag ctgattgaaa ttccggccgg
    31621 aaaacgcggc aagaatgcgg acatcaagcg catactcgac aaagagggtg agcagatgtt
    31681 ggcggccgca ggcaaaaacc gcattgtcac cctcgatatt ccaggcaagc cctgggatac
    31741 gccgcagtta gccgctgagc tggaacgctg gaagctggat ggtcgcgacg tcagtctact
    31801 gattggcggg cctgaagggt tgtcgcctgc ctgtaaagcg gcggcggagc agagctggtc
    31861 gctgtcggcg cttaccctcc cccatccgct ggttcgcgtg ctggtcgcag agagtctgta
    31921 ccgggcgtgg agcatcacca ccaaccatcc ttatcaccgt gagtgataag ggagctttga
    31981 gtagaaaacg cagcggatga aactacagaa ctcttttcgc gactatacgg cagagtccgc
    32041 gctgtttgtg cgccgggcgc tggtcgcctt tttggggatt ttgctgctga ccggcgtgct
    32101 tatcgccaac ctgtataatc tgcaaattgt tcgctttacc gactaccaga ctcgctctaa
    32161 tgaaaaccgc attaagctgg tgcctatcgc gcccagccgc ggcattatct acgaccgtaa
    32221 tggtatccct ctggccctca accgcactat ctaccagata gaaatgatgc cagagaaagt
    32281 cgataacgtg cagcaaacgc tggacgcttt gcgcagcgtg gttgatctga ccgatgacga
    32341 tattgctgca ttccgaaaag agcgcgcacg ttcacaccgt ttcacctcta ttccggtgaa
    32401 aactaacctg accgaagtac aagtagctcg ctttgccgtc aatcagtacc gttttccggg
    32461 tgtcgaagtt aaaggctata aacgtcgtta ctatccttac ggttcggcgt tgacccacgt
    32521 catcggctat gtgtcgaaaa tcaacgataa agacgtcgaa cgcctgaata atgacggcaa
    32581 actggccaac tatgcggcaa cgcatgatat cggtaagctg ggcattgagc gttactatga
    32641 agacgtgctg cacggtcaga ccggttatga agaggttgaa gttaacaacc gtgggcgtgt
    32701 tatccgccag ttaaaagaag taccaccgca agccggacac gatatttacc tgacgctgga
    32761 tctcaaactc cagcaatata ttgaaacgct gctggcgggt agccgcgcag ctgtggttgt
    32821 caccgatccg cgtacaggtg gggtgctggc gctggtttcc acgcctagtt atgacccaaa
    32881 cctgtttgtt gacggtatct ccagcaaaga ttattccgcc ttgttgaacg ccccgaatac
    32941 accgctggtg aaccgcgcca cacagggggt ttatcctccc gcgtctacag ttaaacccta
    33001 tgtggcggtt tcggcattga gcgccggggt gatcacgcgc aatacgacgc tgtttgaccc
    33061 aggctggtgg caactgccag gttcggaaaa acgttatcgt gactggaaaa aatggggcca
    33121 cgggcgtctg aatgtcacaa gatcgctgga agaatctgcg gataccttct tctatcaggt
    33181 ggcctacgat atggggatcg atcgcctctc cgaatggatg ggtaaattcg gttatggtca
    33241 ttacaccggt atcgacctgg cggaagaacg ttccggcaac atgcctaccc gcgaatggaa
    33301 acagaaacgc tttaaaaaac cgtggtatca gggtgacacc attccggttg gtatcggtca
    33361 gggttactgg acagcgaccc caatccagat gagtaaggca ctgatgatcc tgattaatga
    33421 cggtatcgtg aaggttcctc atttgctgat gagcaccgcc gaagacggca aacaggtgcc
    33481 atgggtacag ccgcatgaac cgcccgtcgg cgatattcat tccggttact gggagctggc
    33541 gaaagacggt atgtacggtg ttgctaaccg ccctaacggt acggcgcata aatactttgc
    33601 cagcgcaccg tacaaaattg cggcgaaatc cggtaccgct caggtcttcg gtctgaaagc
    33661 gaacgaaacc tataatgcgc acaaaattgc cgagcgttta cgtgaccaca aactgatgac
    33721 cgcctttgcg ccatacaaca atccgcaagt ggctgtcgcc atgattctgg agaacggtgg
    33781 tgcgggtccg gcggttggta cactgatgcg ccagatcctc gaccacatta tgctgggtga
    33841 taacaacacc gatctgcctg cggaaaatcc agcggttgcc gcagcggagg accattaatc
    33901 atgacggata atccgaataa aaaaacattc tgggataaag tccatctcga tcccacaatg
    33961 ctgctgatct tactggcatt gctggtttac agcgccctgg ttatctggag cgccagcggt
    34021 caggatattg gcatgatgga gcgtaaaatc ggccaaatcg cgatgggtct agtcatcatg
    34081 gtggtgatgg cgcaaattcc tccacgcgtt tatgaaggct gggcccccta tctctatatc
    34141 atctgtatta ttttgctggt ggcggtagat gctttcggtg ccatctctaa aggtgctcaa
    34201 cgctggctgg acctcggtat tgttcgtttt cagccgtcgg aaattgccaa aatagccgta
    34261 ccactgatgg ttgcgcgctt tatcaaccgc gacgtttgcc cgccatcgtt gaagaacact
    34321 ggcatcgcgc tggtgctgat atttatgccc acgctgctgg tggctgcaca gcctgacctg
    34381 ggaacatcaa tcctcgttgc gctttccggt ctgtttgtac tgttcctctc tggccttagc
    34441 tggcgtctga ttggcgtcgc agtagtgctg gtggcggcgt tcattccgat tctgtggttc
    34501 ttcctgatgc atgattacca gcgccagcgc gtaatgatgc tcctagaccc ggaatcagac
    34561 ccactcggcg cgggctatca cattattcag tctaaaattg ctattggctc cggcggatta
    34621 cgcggcaaag gctggctgca cggcactcag tcacagcttg aatttctccc cgaacgccat
    34681 accgacttta tcttcgcggt actggcggaa gagctgggat tagtgggcat tctgattctg
    34741 ctcgctctct acattctgct gatcatgcgc gggctgtgga tagccgccag agcgcaaacc
    34801 acctttggtc gcgtcatggc tggtggctta atgctgatat tattcgttta tgtcttcgta
    34861 aatattggta tggtaagcgg tattctgccg gttgtagggg ttccgctccc actggtcagt
    34921 tatggaggat cggcgctaat tgtgctgatg gctgggttcg ggattgtaat gtcaatccac
    34981 acccacagga aaatgttgtc gaaaagcgtg taagaggtgc gcaatgcgta agcagtggct
    35041 cgggatctgc atcgcggcag gaatgctcgc ggcatgtaca agcgatgatg gtcagcaaca
    35101 gacggtaagt gtaccgcagc ctgcggtatg taacggcccc atagttgaaa ttagcggggc
    35161 ggacccgcgt ttcgaaccac tgaacgcgac ggcaaatcag gattaccagc gcgacggtaa
    35221 aagctacaaa atcgtgcagg atccgtctcg atttagccag gcgggactgg cggcaatcta
    35281 tgatgccgaa cccggcagta acctgacggc ctctggcgaa gctttcgatc cgacacagct
    35341 gacggcggcc catccaacgc ttccgatccc cagctacgcc agaatcacta acctggctaa
    35401 cgggcgaatg atcgtggtgc gcattaatga tcgcggccct tacggcaacg acagcgttat
    35461 ttcactttct cgcgcggcag ctgaccgtct taacacgtca aacaacacca aagttcgtat
    35521 cgatccgatt attgtcgccc aggatggttc gctttctggt cctggtatgg cttgtaccac
    35581 agtcgccaaa cagacttacg ccctgccagc acctcccgat ttaagcggtg gcgcgggaac
    35641 aagttcagtg tctggcccgc agggtgacat tcttccggtc agtaattcga cgctaaaaag
    35701 cgaagatccg accggcgcgc cggtaaccag tagcggtttc ctcggcgctc caacgacctt
    35761 agcgcctggt gtactggaag gcagcgaacc gacgcctgcg ccacagcccg ttgttacagc
    35821 tccgtcgaca acgcctgcaa cctcgcctgc aatggtgaca ccgcaagccg cctcgcaaag
    35881 cgccagcggc aactttatgg tgcaagtcgg ggccgtaagc gatcaggctc gtgcgcaaca
    35941 gtaccaacag caactgggac agaagttcgg cgtccccggt cgcgtaactc aaaatggcgc
    36001 ggtctggcgg atccagcttg gcccattcgc cagcaaagcc gaagccagta ccttgcagca
    36061 acgtttgcaa accgaagccc aattacagtc atttattact accgcgcagt agcgtaaagc
    36121 aggcatctga tgtgtcaatt tctgtaaatg acgttaacaa agtcacgcgg aaagtcagat
    36181 gcctgccggt agtgcatttg ctatagtagg gcactttttt aattccatca cggatgtcgt
    36241 agttcagacc atgaatacca ttttttccgc tcgtatcatg aagcgcctgg cgctcaccac
    36301 ggctctttgc acagccttta tctctgctgc acatgccgat gacctgaata tcaaaactat
    36361 gatcccgggt gtaccgcaga tcgatgcgga gtcctacatc ctgattgact ataactccgg
    36421 caaagtgctc gccgaacaga acgcagatgt ccgccgcgat cctgccagcc tgaccaaaat
    36481 gatgaccagt tacgttatcg gccaggcaat gaaagccggt aaatttaaag aaactgattt
    36541 agtcactatc ggcaacgacg catgggccac cggtaacccg gtgtttaaag gttcttcgct
    36601 gatgttcctc aaaccgggca tgcaggttcc ggtttctcag ctgatccgcg gtattaacct
    36661 gcaatcgggt aacgatgctt gtgtcgccat ggccgatttt gccgctggta gccaggacgc
    36721 ttttgttggc ttgatgaaca gctacgttaa cgcactgggc ctgaaaaata cccacttcca
    36781 gacggtacat ggtctggatg ctgatggtca gtacagctcc gcgcgagata tggcgctgat
    36841 cggccaggca ttgatccgtg acgtaccgaa tgaatactcg atctataaag aaaaagaatt
    36901 tacgtttaac ggtattcgcc agctgaaccg taacggcctg ttatgggata acagcctgaa
    36961 tgtcgacggc atcaaaaccg gacacactga caaagcaggt tacaaccttg ttgcttctgc
    37021 gactgaaggc cagatgcgct tgatttctgc ggtgatgggc ggacgtactt ttaaaggccg
    37081 tgaagccgaa agtaaaaaac tgctaacctg gggcttccgt ttcttcgaaa ccgttaaccc
    37141 actgaaagta ggtaaagagt tcgcctctga accggtttgg tttggtgatt ctgatcgcgc
    37201 ttcgttaggg gttgataaag acgtgtacct gaccattcca cgtggtcgca tgaaagatct
    37261 gaaagccagc tatgtgctga acagcagtga attgcatgcg ccgctgcaaa agaatcaggt
    37321 cgtcggaact atcaacttcc agcttgatgg caaaacgatc gagcaacgcc cgctggttgt
    37381 gttgcaagaa atcccggaag gtaacttctt cggcaaaatc attgattaca ttaaattaat
    37441 gttccatcac tggtttggtt aaaaattgaa cacttgaaag tgtaatttcc gtccccatat
    37501 actaagcatc agtaaaaaac tcccgccttc tggcgggagt tgctatttaa ttacgttacg
    37561 ccggagctga catgaaaacc aaacttaacg aactgcttga attccctact ccttttactt
    37621 acaaagttat ggggcaggcg ttacctgagc tggttgatca ggtggttgaa gtggtacagc
    37681 gccatgcgcc aggtgactac accccaacgg taaaaccaag cagcaaaggc aactaccact
    37741 cggtatctat cactatcaac gccactcata tcgagcaggt tgaaacactg tatgaagaac
    37801 taggcaaaat cgatattgtc cgcatggttc tgtaactcgc ttctccgtta cccggcccct
    37861 ggtcgggtaa ctcccctctc ctcgctgtga tatacttttc ccccactttt actcattctc
    37921 cacggagatg cagttttgta tcaggataaa attcttgtcc gccagctcgg tcttcagcct
    37981 tacgagccaa tctcccaggc tatgcatgaa ttcaccgata cccgcgatga tagtaccctt
    38041 gatgaaatct ggctggtcga gcactatccg gtattcaccc aaggtcaggc aggaaaagcg
    38101 gagcacattt taatgccggg tgatattccg gtgatccaga gcgatcgcgg tgggcaggtg
    38161 acttatcacg ggccggggca acaggtgatg tatgtgttgc ttaacctgaa acgccgtaaa
    38221 ctcggtgtgc gtgaactggt gaccttgctt gagcaaacag tggtgaatac cctggctgaa
    38281 ctgggtatag aagcgcatcc tcgggctgac gcgcctggtg tctatgtcgg ggaaaagaaa
    38341 atttgctcac tgggtttacg tattcgacgc ggttgttcat tccacggtct ggcattaaac
    38401 gtcaatatgg atctttcacc atttttacgt attaatcctt gtgggtatgc cggaatggaa
    38461 atggctaaaa tatcacaatg gaaacccgaa gcgacgacta ataatattgc accacgttta
    38521 ctggaaaata ttttagcgct actaaacaat cctgacttcg aatatattac cgcttaattg
    38581 catacctcaa tggcccaatt tacactgggt cattactcat aatacctgct aatgctttac
    38641 atctccttcg cgtttgtata ctttctttat cacttgcaga aatgcggtgc tacgccttgc
    38701 tccgtaactc accccattga ttaacctatt gaaatagttg attatggtga gcaaaagcgt
    38761 tccgttccaa ttagacataa gattattgta atttttttaa atattatttt tccatgaata
    38821 aaattggagt aagtgcgtgg atagtaataa tcaaattgaa ccctgtttaa gcagaaagtc
    38881 gtcagaaggt aaaccacaaa tatttacaac cttacgaaat attgatctta accttctgac
    38941 tatttttgaa gctgtatatg tacataaagg gatcgttaat gcagcgaaag tgcttaatct
    39001 gaccccctcg gcaatcagtc agtctattca gaaactgcgc gttatattcc ctgacccatt
    39061 gtttattcgc aaaggccagg gtgtcactcc taccgcattt gcgatgcatc tacatgagta
    39121 tatcagtcag ggccttgagt ccattcttgg cgcgctggat atcgaaggaa gctatgataa
    39181 gcaacgaacg ataactattg cgaccactcc ctcggtcgga gccctggtcc ttcctgtcat
    39241 ctatcgggcg attaaaactc actatccgca gcttttactg cgcaacccgc ccatcagcga
    39301 cgcggaaaac caactcagtc agtttcaaac cgatctcatc atcgataaca tgttttgcac
    39361 caatcgtacg gtgcaacatc atgttctgtt caccgacaat atggtgttaa tttgccgcga
    39421 gggaaatcca ctactctctt tagaagatga cagagagact atcgacaacg ctgcgcatgt
    39481 actcctgtta ccggaagggc aaaatttcag cggtctgcgg cagagagttc aagagatgtt
    39541 tccggaccgg caaatcaatt tcaccagcta caacattttg acaatcgctg cactggttgc
    39601 caacagtgac atgttagcga ttattcccag ccgtttttat aacctgttta gtcgttgctg
    39661 gccgctggaa aaactgcctt ttccgtcctt aaatgaggag caaatagatt tctctatcca
    39721 ctacaacaaa ttcagcctgc gtgatcccat cctgcacggg gtaatagatg ttatccgtaa
    39781 tgcattttga aaaagtattg ttacagccag atcacagcgc agaacgataa aatggcacaa
    39841 tccaacaaca attttacatt ttcgcgaccg ctttggctgc tttcaggtcc gtttcaatga
    39901 tatactgcca gtcgttaatt caaaaatagt tgataattac aacaatctat tgaattgaaa
    39961 cgctttcctt cgtaattcgc aactggaaca cgcacgctat gagtaaaccc attgtgatgg
    40021 aacgcggtgt taaataccgc gatgccgata agatggccct tatcccggtt aaaaacgtgg
    40081 caacagagcg cgaagccctg ctgcgcaagc cggaatggat gaaaatcaag cttccagcgg
    40141 actctacacg tatccagggc atcaaagccg caatgcgcaa aaatggcctg cattctgtct
    40201 gcgaggaagc ctcctgccct aacctggcgg aatgcttcaa ccacggcaca gcaacgttta
    40261 tgatcctcgg cgctatttgt acccgccgtt gtccgttctg tgatgttgcc cacggtcgcc
    40321 cggtagctcc tgatgccaat gaaccagtga aactggcgca gaccattgcc gatatggcgc
    40381 tgcgttatgt ggttatcacc tccgttgacc gtgatgacct gcgcgatggc ggtgcccagc
    40441 actttgcgga ttgcattact gccattcggg aaaaaagccc gcaaatcaaa attgaaactc
    40501 tggtgccgga tttccgtggt cgtatggatc gtgctctgga tattctgact gcaacgccac
    40561 cagatgtgtt caaccataac ctggaaaacg taccgcgtat ttaccgtcag gtacggcctg
    40621 gtgcagatta caactggtcg ctgaagctgc tggaacgctt taaagaagcg catccggaaa
    40681 tcccgaccaa gtctggtctg atggtgggac tgggtgaaac caatgaagaa attattgagg
    40741 taatgcgcga cctgcgccgt catggtgtga cgatgttaac gctggggcaa tatttgcagc
    40801 caagccgcca tcacctgccg gttcaacgtt acgttagccc ggatgagttc gacgaaatga
    40861 aagccgaagc gctggcgatg ggctttaccc atgctgcatg cggtccgttt gtccgctctt
    40921 cttaccacgc cgatttgcag gcgaaaggga tggaagttaa gtaatcctgt aataattact
    40981 tacatctgtc tgataaaaaa cccgcttttt
//
LOCUS       DAEEKQ010000017        40133 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.017, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000017 DAEEKQ010000000
VERSION     DAEEKQ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 40133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40133
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.017"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..494
                     /gene="gstA"
                     /locus_tag="KLK57_000919"
     CDS             <1..494
                     /gene="gstA"
                     /locus_tag="KLK57_000919"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBE0891363.1"
                     /translation="NGDDYFAVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQL
                     LAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEAL
                     KDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAE
                     GLK"
     gene            complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="KLK57_000920"
     CDS             complement(538..1401)
                     /gene="pdxY"
                     /locus_tag="KLK57_000920"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HBE0891364.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEVLEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="KLK57_000921"
     CDS             complement(1460..2734)
                     /gene="tyrS"
                     /locus_tag="KLK57_000921"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HBE0891365.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="KLK57_000922"
     CDS             complement(2863..3519)
                     /gene="pdxH"
                     /locus_tag="KLK57_000922"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HBE0891366.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="KLK57_000923"
     CDS             complement(3578..3907)
                     /gene="mliC"
                     /locus_tag="KLK57_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HBE0891367.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGVRYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="KLK57_000924"
     CDS             complement(4005..5114)
                     /gene="anmK"
                     /locus_tag="KLK57_000924"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HBE0891368.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            5388..5855
                     /gene="slyB"
                     /locus_tag="KLK57_000925"
     CDS             5388..5855
                     /gene="slyB"
                     /locus_tag="KLK57_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HBE0891369.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="KLK57_000926"
     CDS             complement(5902..6336)
                     /gene="slyA"
                     /locus_tag="KLK57_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HBE0891370.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            6537..6773
                     /locus_tag="KLK57_000927"
     CDS             6537..6773
                     /locus_tag="KLK57_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HBE0891371.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            6776..7633
                     /locus_tag="KLK57_000928"
     CDS             6776..7633
                     /locus_tag="KLK57_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0891372.1"
                     /translation="MSIKTIKYFSTIIVAVVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     RVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            7654..9645
                     /locus_tag="KLK57_000929"
     CDS             7654..9645
                     /locus_tag="KLK57_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBE0891373.1"
                     /translation="MRNLPWFRATLAQWRYALRNTIAMCLALTVAYYLNLDEPYWAMT
                     SAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSAWLGFCTW
                     ACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGILCGGMMM
                     MILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQILTMNLLRI
                     QAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREILEQLLTAL
                     ASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRLQSGVDDH
                     ARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLAAISCVLY
                     SAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLATMQLLKLQ
                     MPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNENLAKIVGVALAWLAFAILRPGSD
                     ARKSRRHIRALRRDFVDQLSRHPTLSGSEFESLTYHHVSQLSNSQDALARRWLLRWGV
                     VLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATLEELQRIC
                     DSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="KLK57_000930"
     CDS             complement(9646..10167)
                     /gene="sodC"
                     /locus_tag="KLK57_000930"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HBE0891374.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(10248..11144)
                     /locus_tag="KLK57_000931"
     CDS             complement(10248..11144)
                     /locus_tag="KLK57_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HBE0891375.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLKSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(11193..11432)
                     /locus_tag="KLK57_000932"
     CDS             complement(11193..11432)
                     /locus_tag="KLK57_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HBE0891376.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            11535..12134
                     /gene="nemR"
                     /locus_tag="KLK57_000933"
     CDS             11535..12134
                     /gene="nemR"
                     /locus_tag="KLK57_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HBE0891377.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKIAEVPKGS
                     FYHYFRSKEAFGVAMLERHYATYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGHENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRNSEPLENALAHVKTIIATPAV"
     gene            12171..13268
                     /gene="nemA"
                     /locus_tag="KLK57_000934"
     CDS             12171..13268
                     /gene="nemA"
                     /locus_tag="KLK57_000934"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HBE0891378.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRRAELNPQRAESFYGGGAEGYTDYPTL"
     gene            13349..13756
                     /gene="gloA"
                     /locus_tag="KLK57_000935"
     CDS             13349..13756
                     /gene="gloA"
                     /locus_tag="KLK57_000935"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HBE0891379.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            13859..14506
                     /gene="rnt"
                     /locus_tag="KLK57_000936"
     CDS             13859..14506
                     /gene="rnt"
                     /locus_tag="KLK57_000936"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HBE0891380.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            14599..19168
                     /locus_tag="KLK57_000937"
                     /pseudo
     CDS             14599..19168
                     /locus_tag="KLK57_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
     gene            complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="KLK57_000938"
     CDS             complement(19219..19566)
                     /gene="grxD"
                     /locus_tag="KLK57_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HBE0891381.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGKLVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            19900..20715
                     /gene="mepH"
                     /locus_tag="KLK57_000939"
     CDS             19900..20715
                     /gene="mepH"
                     /locus_tag="KLK57_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HBE0891382.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            20843..21424
                     /gene="sodB"
                     /locus_tag="KLK57_000940"
     CDS             20843..21424
                     /gene="sodB"
                     /locus_tag="KLK57_000940"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HBE0891383.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(21570..22739)
                     /locus_tag="KLK57_000941"
     CDS             complement(21570..22739)
                     /locus_tag="KLK57_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0891384.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLPALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLATPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="KLK57_000942"
     CDS             complement(22905..22994)
                     /gene="ynhF"
                     /locus_tag="KLK57_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HBE0891385.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            23293..24318
                     /gene="purR"
                     /locus_tag="KLK57_000943"
     CDS             23293..24318
                     /gene="purR"
                     /locus_tag="KLK57_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HBE0891386.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLVGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(24315..25247)
                     /locus_tag="KLK57_000944"
     CDS             complement(24315..25247)
                     /locus_tag="KLK57_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0891387.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            25360..26571
                     /locus_tag="KLK57_000945"
     CDS             25360..26571
                     /locus_tag="KLK57_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HBE0891388.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGMTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIIPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEAGCQNH
                     GNAEVAHSESH"
     gene            26862..28010
                     /gene="cfa"
                     /locus_tag="KLK57_000946"
     CDS             26862..28010
                     /gene="cfa"
                     /locus_tag="KLK57_000946"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HBE0891389.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="KLK57_000947"
     CDS             complement(28050..28691)
                     /gene="ribE"
                     /locus_tag="KLK57_000947"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HBE0891390.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            28906..30279
                     /gene="mdtK"
                     /locus_tag="KLK57_000948"
     CDS             28906..30279
                     /gene="mdtK"
                     /locus_tag="KLK57_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HBE0891391.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(30320..31576)
                     /locus_tag="KLK57_000949"
     CDS             complement(30320..31576)
                     /locus_tag="KLK57_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HBE0891392.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            31884..31960
                     /locus_tag="KLK57_000950"
     tRNA            31884..31960
                     /locus_tag="KLK57_000950"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31918..31920,aa:Val,seq:gac)
     gene            31965..32041
                     /locus_tag="KLK57_000951"
     tRNA            31965..32041
                     /locus_tag="KLK57_000951"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31999..32001,aa:Val,seq:gac)
     gene            32149..32454
                     /locus_tag="KLK57_000952"
     CDS             32149..32454
                     /locus_tag="KLK57_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HBE0891393.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            32580..34184
                     /locus_tag="KLK57_000953"
     CDS             32580..34184
                     /locus_tag="KLK57_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HBE0891394.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFHRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EYGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(34196..35008)
                     /locus_tag="KLK57_000954"
     CDS             complement(34196..35008)
                     /locus_tag="KLK57_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891395.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIVTAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(35012..35797)
                     /locus_tag="KLK57_000955"
     CDS             complement(35012..35797)
                     /locus_tag="KLK57_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HBE0891396.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMCGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(35794..36462)
                     /locus_tag="KLK57_000956"
     CDS             complement(35794..36462)
                     /locus_tag="KLK57_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBE0891397.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQENKYYQYQLLGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(36526..37164)
                     /locus_tag="KLK57_000957"
     CDS             complement(36526..37164)
                     /locus_tag="KLK57_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HBE0891398.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(37177..39285)
                     /locus_tag="KLK57_000958"
     CDS             complement(37177..39285)
                     /locus_tag="KLK57_000958"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HBE0891399.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKIKDFYYRLAHRVGELSHLADGSYAIAER
                     WNLGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGL
                     PLKLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPM
                     TVSPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDM
                     RNEHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETL
                     QRLGLEDIAADLAAHNLLPA"
     gene            complement(39306..39932)
                     /locus_tag="KLK57_000959"
     CDS             complement(39306..39932)
                     /locus_tag="KLK57_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HBE0891400.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         9631 a        10550 c         9885 g        10067 t
ORIGIN      
        1 aaaacggtga cgattacttt gccgttaacc ctaaggggca ggtgccagca ttgctgctgg
       61 atgacggtac tttgctgacg gaaggcgtag cgattatgca gtatcttgcc gacagcgtcc
      121 ccgaccgcca gttgctggca ccggtaaaca gtatttcccg ctataaaacc atcgaatggc
      181 tgaattacat cgccaccgag ctgcataaag gtttcacacc tctgtttcgc ccagatacac
      241 cggaagagta caaaccgaca gttcgcgcgc agctggataa gaagctgcaa tatgtgaacg
      301 aggcactgaa ggatgagcac tggatctgcg ggcaaagatt tacaattgct gatgcctatc
      361 tgtttacggt tctgcgctgg gcatacgcgg tgaaactgaa tctggaaggg ttagagcaca
      421 ttgcggcatt tatgcaacgt atggctgaac gtccggaagt acaagacgcg ctgtcagcgg
      481 aaggcttaaa gtaatagcca tcggcccaca ggtcgttgtg ggccgaaatg tgatatttca
      541 gagctttgtt gcgctgaagt aatgttctgg tttggcaata cgatcctgag cagccaccac
      601 ttgcagctca tattcctgca ttgctttggt ggtcaccatg atttcgtaga ctgcagcggt
      661 cacatgttcc aggacctcct gcagcgttgc cccctgaagc agtttcacca gcagtaaacc
      721 gctcgtcaca tcaccaacac ctaccggctg gcgcatacca aaatccacca gtggacggct
      781 gatatgccag gcttcatcgg cggtgaccag cagcatttca aaacggtcac ggctgtagcc
      841 agctcgcgcc aggtgtttaa ccaacacaat ttgtggccct tgcgcaatga gttcgcgcgc
      901 tgccagaacc gcttcttcga cgttatttac cgcatgctca cagagtattt ccagctcaac
      961 cagattcggc gcaatgatat cgctggcagg caaaccgtgc cgcacatgaa actctgcgac
     1021 acccggtgca acgatacagc ctttttccgg atgccccatt accggatcgc aaaaatattt
     1081 cgcctgcgga ttcgctgctt tcacctgacg gacgataccg aggatatgtt caccctgctc
     1141 cgccgatccc agatagccac ttaacacggc atcacaggtg tgtaatttat caatagcggc
     1201 aatgccttgc acaatttcgg ttaaatggct gggcggcatc acgcagccag tccatttgcc
     1261 gtattgggtg tgattagaaa attgaacggt gttcagcggc cagacgttcg cgcccaggcg
     1321 gcgcatcgga aactctgccg cactgttacc cgcatgacca taaacaacgt gagactggat
     1381 agcgagaata tttttcatca tgttccctgt ataaaaacca gggggagtga tttctcactc
     1441 cccctttcca cttaatgcat tatttccagc aaatcagaca gtaattcttt ttgccgcgac
     1501 gcagtaaggt aaaacgacca aacagacgat cttcttcttt aaagaagtat tcaggatcgg
     1561 actgtttttc accgttaatg gtgatggcat tggaggcgat agttttacgc gcctgaccac
     1621 gggaaggttg cagttcagaa tcgaccagcg cctgcatcag gtctgcgccc ttttccatct
     1681 caaccatcgg tacgccgtcc tgcgccagct gttcgaagtc cgcttcactc agcgcactca
     1741 aggaaccgct gaacaggcat tcggtaatac gttttgccgc ctgcaaacct tcttcaccgt
     1801 gaaccagacg agtcacctgc tccgccagta catactgggc gcgcggtgct ttaccgctgt
     1861 ttttatcttc ttcttccagg gcgttgatct cttcaatgct cataaaggtg aagaacttca
     1921 ggaagcggta aacgtcggca tccgcagtgt tgatccagaa ctggtagaat ttgtacgggc
     1981 tggttttctt cggatccaac cagactgcgc cgccttcagt tttaccaaat ttggtgccgt
     2041 ctgctttggt gatcagcgga acggtcaggc caaacacctg attctgatgc agacgacggg
     2101 tcaggtcgat accagaagtg atgttacccc actggtcaga accaccaatt tgcagcacca
     2161 caccgtactg tttgttcaga caggcgaagt cataaccctg caacaggttg taggaaaact
     2221 cagtgaacga aatcccctga tcttcacggt tgagacgctg cttaaccgct tctttgttga
     2281 tcatctggtt aacggagaag tgtttgccaa tatcgcgcag gaaggtcagc acattcatat
     2341 tgccgaacca gtcatagttg ttcgccgcga tagcagagtt ttctccacag tcgaaatcaa
     2401 ggaacggggc aacctgcttg cggattttgt ccacccactc ctgaacagtt tcttcggtgt
     2461 tcagcttacg ctcggcagct ttgaagctcg ggtcgccaat cagacccgtc gcgccgccta
     2521 ccagcgcaac cggcttgtgg cccgcctgct ggaagcgttt caggcataac aatggaacaa
     2581 gatgccccaa atgcaagctg tcagcggtag gatcgaagcc gcaatagagc gcgatcgggc
     2641 cttgcgccag tcgctctgct aacgcttcct cgtccgtcac ctgggctacc agcccccgct
     2701 cttgcaattg tttaatcaag ttactgcttg ccatcaaaat ctccatgtat ataacgactg
     2761 cacctttgcc ggtacacgac ttttcgccag atgcgaaaga gacatagaat aaagtgccag
     2821 aatcaggagt accagcgatt aaagcaagat ttttgcatct tttcagggtg caagacgatc
     2881 aatcttccac gcatcatttt cacgctggta caaaaagcgg tcatgcaggc gatgctcacc
     2941 gccctgccag aactcaatct gttcaaggct gacgcgaaaa ccgccccaaa agctcggcaa
     3001 tggcacttcg ccctgttgaa acttctgctt cagctccagg aatttacttt caaggatacc
     3061 gcgggcagaa atgcgactgg actgcttcga aacccatgca ccaatctggc tatcacgcgg
     3121 gcggctatga aaatatttca tcacttcgag agtcgaaagt cgttccgctt taccgatcac
     3181 catcacctgg cgctcaaggg tatgccacgg gaacagcagg ctaacgcgcg gattattttc
     3241 aatttgatgt gctttacggc tgccgaggtt ggtgtaaaac accatgcctt tttcgtcgta
     3301 atgtttgagt aaaacgatgc gttgataagg ctgagcatgt tcatccacgg tagcgaccac
     3361 catcgcggta gggtctgcca gtttggcttc acacgcctga gagagccaac gttcaaaaag
     3421 ggttaatgga tcggcgggaa gatcgcgacg gcgtaacccg cctttggtgt attcacggcg
     3481 cagatgcgcg atttgctgca attcgtcgtt atcagacatg gttttcttta cggattgtca
     3541 gtgggtgacg ctattgtgcg ccgcccctgg aaaaatctca acgctgtgga ttttgtaact
     3601 gacagttatt caatacgatg cggtcgcgtt tatagacagt cgcttcatcg cctttcgacc
     3661 agaaaacata gattccgtca gtgtaacgta caccagacgc tgaaatgccc tgtttgagat
     3721 gcagtagttg attatcgtaa acaaaactga cttcctggcg cggattattc agtttgaccg
     3781 tcaacggttt ttcatcacac tggtattcca gcgtagcggt ttgcatgtgc tcaaccagtt
     3841 gattgaacgc gctacagccg gtgagaagca ccggcaaaca gatcagtaac agttttttca
     3901 tagacatttc ccgaagactt tcctggtctg gagggcaata cgccctccct aacgtttcaa
     3961 gtgtaacggc agacgcggta agaaaaattc agttaactct gatatcacgg gttagcaggg
     4021 aaaatagccc ccagtaccgt ctcctggctt gcgccagtga cggaaggcag atttcctggt
     4081 aatcccgcca gcgtccgcca ggcaagccag gcgaatgcca atgcttccat gtcatcgcca
     4141 ctaatgccaa cggcatcggt ggtggtgact tctgtgcctg gcagtaatgc cgccagacgc
     4201 gccatgagta gcgggttacg actacctcca ccacatacca tcaatcgttc gcagccaccg
     4261 ctcaacaaaa cttgttcaga aatggtcacg gcggtgagtt ctgccagtgt ggcctgaaca
     4321 tcacggggat caacacccgg aaaatggcgt aaatggcgct ccagccagcc gtagttaaag
     4381 tattcgcgtc cggtgctttt cggtgcaggt tgcgagaaat atgggtcgct gagcatattt
     4441 tgcagcagtg ggagaataac tttacccgcc cgtgcccact cggcatcttt atcgtaaggt
     4501 ttaccggcct gacgccagat ccaggcatcc atcagcatgt taccaggacc ggtatcgtag
     4561 ccgccaaccg gctgcccagg aatgagcagt gacagattgg cgatgccgcc aatattgagc
     4621 accattcgtc gctcggttgg gtgagccagc aaggcatgat ggaacgcagg taccagcggt
     4681 gcgccttgtc ctcccaaggc aatatcgcga cggcgaaaat caccgacaac cgtaattccg
     4741 gtgcgtgcca caatttgatt gttatcgcca atctgtaaag tgtgtggtgc cacgcccgtc
     4801 ggttcatgcc agacggtttg accgtgacag ccgatcgcaa ctatatctct cgcctgcagg
     4861 ttttgttcct taagcaaggc attgaccgca tcagcaaaaa gtcgcccgag ttgagtatca
     4921 agctgtccaa actgcgaaag tgtaagctgc tggccctggc aaatatccag tacagcctgt
     4981 ttcagagaca ccgggatcgg ccagctcaag cttgccagct gtgcgacccg gtgttcatct
     5041 attgtcgcca acacaacatc aacaccatca aggctggtgc ctgacataac gccaataaag
     5101 cggcccgatt tcatagttca tcctttttca atctgacgtt tgcgcaccac tcaaacataa
     5161 acttttcgtg gataccatgc ggaatgaccg atttttaccg ttggtagtaa aacattatct
     5221 tcaaatcaat aatcatcatg aatgttttgt ttataattgg ttgatcctac tttcattatg
     5281 atttgctcat atttggtaga acatgtaacc atggattcac atatgccata tactttgacc
     5341 atgagggatg cttgcgtggc gtttcatggt gaacaggaga tttttcaatg attaaacgcg
     5401 tattggttgt ttcaatggta ggtctgtctc ttgtcggttg tgttaataac gacaccctgt
     5461 caggggatgt ttataccgct tctgaagcga aacaagtaca gaatgtcagc tatggcacca
     5521 tcgttaacgt acgtccggta cagattcagg gcggtgatga ttccaacgtt atcggtgcaa
     5581 ttggcggtgc tgttcttggt ggtttcctgg ggaatactgt tggtggcgga accgggcgtt
     5641 ctctggctac tgcagcaggc gctgttgcag gtggcgtagc tggtcagggc gtacagagtg
     5701 caatgaacaa aacgcagggt gtcgagctgg aaattcgtaa agacgatggt aataccatca
     5761 tggtggtaca gaaacaaggc aacactcgtt tctctccggg ccaacgtgtc gtactggcca
     5821 gcaatggcag tcaggtgacc gtttctccgc gctaaataag tttgcgtgtg gtcaggttac
     5881 tgaccacacg cccccttcat ttcacccttt ggcctgtaac tcaatgatat tatgctcaag
     5941 ttttgcgatg agcgtaatca gttgctccag ttcctctgcg gagatgccat gtaatatttc
     6001 cgcgcgggtt ttgttaataa cagcttccat ttcgctgatc agtggctctg ccttttccgt
     6061 cagtttaata cgtttagccc gacgatcgct ggcacaagtt tgacgcgaaa ttaacccttt
     6121 ttcttccagt tggtccagag tacggaccag tgatggctgc tcgatgccaa tcgcttttgc
     6181 cagttgaatt tgcgactggt ctggaggtaa ctgatggata ttgtgtaacg taacccaatg
     6241 ggtttgtgtt aactccagcg gtttcaggcg atggtctatc agagcacgcc atatgcgcac
     6301 caaccgtgcc agatcagaac ctagtggcga ttccaatttc atctccttat aattagcttg
     6361 ctaagatatt atgcggcttt tagaatagtg tgcagcaatt gtattgctaa aacaaatgta
     6421 ttgctgcatt tggttaccgt cagacatatt tttcagaaat tgcgcgtaaa tttttcgcac
     6481 ttaaagaata tttattaatc taacgcaata tattcggtcg taaaggaatc tactttgtga
     6541 agtttatgct caatgcaaca ggattgccct tacaagatct ggtgttcggt gcgtccgtct
     6601 attttcctcc gtttttcaaa gcattcgcgt ttggattcgt catctggctt gtcgtacacc
     6661 gcctgcttcg tggctggatc tacgccggtg acatctggca tcccttgtta atggatttat
     6721 cgctgtttgc gatttgcgtt tgccttgctc tggcaatact gattgcgtgg taactatgtc
     6781 aattaaaaca attaagtatt tctcaacaat cattgtagcg gtagttgcgg ttcttgccgg
     6841 atggtggctg tggaattatt acatgcaatc tccgtggaca cgcgacggga aaatacgcgc
     6901 agaacaggtt tctatcaccc cacaggtgtc gggacgtatt gttgagctga atataaaaga
     6961 caaccagctg gttaacgcag gggatcttct gctcacgatc gataaaacgc cctttcagat
     7021 cgccgagctg aacgcacagg ctcagctggc gaaagcgcaa tctgacctcg caaaagccaa
     7081 taacgaagcg aatcgtcgtc gtcatctctc acaaaatttt atttctgccg aagaactcga
     7141 taccgctaac ctcaatgtta aagcgatgca ggccagtgtt aatgccgcac aggccacgct
     7201 gaaacagaca caatggcaac tggcgcaaac ggaaattcgc gccccggtga gtggacgggt
     7261 gactaacctc actacccgca tcggcgatta cgctgacacc gggaaaccgc tttttgccct
     7321 tgtcgacagc cactcgtttt acgtcattgg ttattttgaa gaaaccaagt tgcgccatat
     7381 ccgcgaaggt gcaccagcac aaattaccct ctatagtgat aacaaaacgt tacagggtca
     7441 cgtttccagt atcggtcggg cgatttatga tcagagcgtt gaaagtgact caagccttat
     7501 tccggatgtg aaacctaacg tcccctgggt tcgtctcgcc caacgcgttc ccgtccgctt
     7561 tgcgctggat aaagttccag gcgatgtcac gctggtgtcg ggcaccacct gtagcattgc
     7621 cgtaggtcaa taatgaacgc atcgtaatgg tccttgcgca atttgccctg gttcagggcc
     7681 acgctggcgc aatggcgtta tgcgttacgc aataccattg ccatgtgtct ggcgctgacg
     7741 gttgcctatt atttgaatct ggatgaaccc tattgggcga tgacctcggc tgcagtggtt
     7801 agctttccca ccgttggcgg tgtcatcagc aaaagcctcg gacgcatcgc tggcagttta
     7861 ctgggagcca ttgcggcact gcttcttgcc ggacatacgc tcaatgagcc gtggtttttt
     7921 ctattgagca tgtcggcgtg gcttggcttt tgtacctggg cctgtgcgca cttcacgaat
     7981 aacgtcgcgt atgcatttca actggcgggc tacacggctg ccatcatcgc ctttccgatg
     8041 gttaatatta ctgaggccag ccagctgtgg gatatcgctc aggcgcgcgt ttgcgaggtg
     8101 atagtcggta ttttgtgcgg cggcatgatg atgatgatcc tgcctagcag ttccgatgct
     8161 actgcccttt taactgcatt gaaaaacatg cacgcccgat tactggaaca tgccagttta
     8221 ctctggcagc ctgaaacaaa cgatgccatt cgtgcagcac atgagggggt gattgggcag
     8281 atactgacca tgaatttgct gcgtatccag gctttctgga gccactatcg ttttcgccag
     8341 caaaacgcgc gccttaatgc gctactccac cagcaattac gtatgaccag tgtcatctcc
     8401 agcctgcgac gtatgttgct caactggccc tcaccgccag gtgccacgcg agaaattctc
     8461 gaacaattgc tgactgcgct cgccagttcg caaacagatg cttacaccgt cgcacgtatt
     8521 atcgccccgc tacgcccgac caacgtcgcc gactaccggc acgtcgcctt ctggcagcga
     8581 ctacgttatt tttgccgcct ttatctgcaa agtagtcagg aattacatcg tctgcaaagc
     8641 ggtgtagatg atcatgccag actcgcacgg acatccgctc tggctcgtca taccgacaac
     8701 gccgaagcta tgtggagcgg gctgcgtaca ttttgtacgt tgatgatgat tggcgcatgg
     8761 agtattgcat cgcaatggga tgccggtgcc aatgcattaa cgctggcagc aattagctgc
     8821 gtactctact ccgccgtcgc agcaccgttt aagtcgttgt cactgctgat gcgcacgctg
     8881 gtgttacttt cgctattcag ctttgtggtc aaatttggcc tgatggtcca gattagcgat
     8941 ctgtggcaat ttttactgtt tctctttcca ctgctggcga caatgcagct tcttaaattg
     9001 cagatgccca aatttgccgc actgtggggg caactgattg tttttatggg ttcgtttatc
     9061 gctgtcacta atcccccggt gtatgatttt gctgattttc ttaacgagaa tctggcaaaa
     9121 atcgttggcg ttgcgctggc ttggctagcg ttcgccattc tgcgtccagg atcggatgct
     9181 cgtaaaagcc gccgccatat tcgcgcgctg cgccgggatt ttgtcgatca gctaagccgc
     9241 catccaacac tgagtggaag cgaatttgaa tcgctcactt atcatcacgt cagtcagttg
     9301 agtaacagcc aggatgcgct ggctcgccgt tggttattac gctggggtgt agtgctgctg
     9361 aactgttctc atgttgtctg gcaattgcgc gactgggaat cgcgttccga tccgttatcg
     9421 cgagtacggg ataactgtat ttcactgttg cggggagtga tgagtgagcg tggcgttcag
     9481 caaaaatcac tggcggccac acttgaagaa ttacagcgga tttgcgacag ccttgcccgt
     9541 catcatcaac ctgccgcccg tgagctggcg gcaattgtct ggcggctgta ctgctcgctt
     9601 tcgcaacttg agcaagcacc accgcaaggt acgctggcct cgtaattact taattacacc
     9661 acaggcatag cgttcaccgc caccgcccag cggtttaggt tgatcggaca tattatcgcc
     9721 gccaacgtgg accatcagcg ccttgtcttt gatttcatcc agtgatttca gacgaggcgc
     9781 gatgacggca tcggtagctt tgccgtcatt attgacgacc agtgcaggca gatcgcctaa
     9841 atgcccggta ccttctggcc cttcatgttt accggtattt tgtggatcaa gatgcccgcc
     9901 tgcggattcc gcggcgctgg ctttgccatc tttggtggct ggctggcagc ttcctttggc
     9961 atgaatatgg aagccatgtt caccgggggg taatgctttc agatcgggtg aaaactccag
    10021 acctttatcg gtttcagtaa tggtgacgct accaattgac tgcccaaccc cttgcgacgt
    10081 gacgaggttc atctcgactt tttcactggc agcttgtgcg ccggtcgcaa caaccagcgc
    10141 cagaatggcc agactaaaac gtttcatagg acctccgttc attgtgcatt cctgaataag
    10201 tgtaaaccag tgacacattt ttgaacggcg gctattccta aaagtgctta cggtacgtcg
    10261 taccccagtg ccgctttacg gatacgaaac cattgttgac gggtcatttt cagtgtttct
    10321 gcttcgacag ctgcccgtac gcgctcaatt ttacctgagc cgataattgg cagcggctgc
    10381 gacggtaaac gtaataccca ggcgtaaacc acctgttcaa tcgagcccgc gtttaactcc
    10441 tccgccacca cagccagttc atcacgcagc ggctggaaat aatcatcatt aaacagacga
    10501 ccaccaccaa ggcaggacca cgccatcgga cgaacacgca gttgttgtag ttggtcgagc
    10561 gtgccatcca gcagtaacgg ctgatgcacc ggggatattt ccacctgatt agtggcaagg
    10621 gtaaacggca gacgtgattt caacagggca aattgcgcag gcgtaaagtt cgatacgcca
    10681 aaatgacgca ctttgccgct ctgatgcaga tgtttgaacg cgtccgccac ttcatcggca
    10741 tccattaacg ggtctggtcg gtggattaac agcagatcca gatgatcggt cgcgagatta
    10801 attagcgact gttcggcgct cttaatgatg tgatcgcggt cagtgatgta atgaccaatg
    10861 acgttttctt cacgcgcggt cgtcgcgata ccgcatttac tgacgatttc catccgttca
    10921 cgcaggtgag gtgccagttt cagtgcctcg ccaaacgccg cttcgcactg atagccacca
    10981 taaatatcag catggtccac ggtggtcacg ccgagatcca gatgctcttc aataaaactg
    11041 accagctggc gggcggacat attccagtcc atcaatcgcc agtagcccat cacaaaacgg
    11101 gaaaactccg ggccttgcgg cgcaatagta atacgctgaa ccataatcgc ttcctcttat
    11161 cagatatgag aggagtatac gcaagattag gttcaaaaga gtgatggttg ctccggttcg
    11221 tctgatgacg ctggcttatt tgcgcgtaat ttgcgcatta atcgctgccg acaaaggcgc
    11281 agcacctctt gtttttcgcc atcgctcatt ttattccagt taaaacgctc atcccgacta
    11341 cgaaaacagc cgcgacaaaa cccgcgttcg tcagactggc aaattccccg gcacgggctc
    11401 tggacgggaa agaactctaa ttgctccgcc acttcgccct cctcaaataa gattattacc
    11461 attattgaag ctgttaatgt ccaaagtagc aactttgctt gcactagacc gactggtcta
    11521 ctacactcca acgcatgaac aaacacaccg aacatgatac tcgcgaacat ctcctggcga
    11581 cgggcgagca actttgcctg caacgtgggt tcaccgggat ggggctaagc gaattactaa
    11641 aaatcgctga agtgccgaaa gggtccttct atcactactt tcgctctaaa gaagcgtttg
    11701 gcgttgctat gcttgagcgc cattacgcca catatcacca gcgactgact gagttgctgc
    11761 aatccggcga aggtaactac cgcgaccgca tactggctta ttaccagcaa acactgaacc
    11821 agttttgcca acatggaacc atcagtggtt gcctgacagt aaaactctct gccgaagtgt
    11881 gcgatctgtc agaagatatg cgtagcgcga tggataaagg cgctcgcggc gtgattgccc
    11941 tgctctctca ggcgctggaa aatggccatg agaaccactg tttaaccttt tgtggcgaac
    12001 cgctgcaaca ggcacaagtg ctttacgcac tatggctggg tgcgaatctg caggccaaaa
    12061 tttcgcgcaa ttccgagcca ctggaaaacg cgctggcaca tgtaaaaacc attattgcga
    12121 cgcctgccgt ttagcaggca ttttttatca ccagacgacc gggagccttt atgtcatctg
    12181 aaaaactgta ttccccactg aaagtgggcg caataactgc ggcaaaccgt atttttatgg
    12241 caccgctgac gcgtctgcgc agtattgaac cgggtgacat ccctaccccg ttgatggcgg
    12301 aatactatcg ccaacgtgcc agtgccggtt tgattattag cgaagccacg caaatttctg
    12361 cccaggcaaa agggtatgct ggtgcacctg gcatccatag tccggaacaa attgccgcat
    12421 ggaaaaaaat taccgctggc gttcatgctg aaaatggtca tatggccgta cagctgtggc
    12481 acactggacg catttctcac gccagcctgc aacctggcgg tcaggcaccg gtagcgcctt
    12541 cagcacttag cgcgggaaca cgtacttctc tgcgcgatga aaatggtcag gcgatccgtg
    12601 ttgaaacatc catgccgcgt gcgcttgaac tggaagagat tccaggtatc gtcaatgatt
    12661 tccgtcaggc cattgctaac gcgcgtgaag ccggttttga tctggtagag ctccactctg
    12721 ctcacggtta tttgctgcat cagttccttt ccccttcttc aaaccatcgt accgatcagt
    12781 acggcggcag cgtggaaaat cgcgcacgtt tggtactgga agtggtcgat gccgggattg
    12841 aagaatgggg tgccgatcgc attggcattc gcgtttcgcc aatcggtact ttccagaaca
    12901 cggataacgg cccgaatgaa gaagccgatg cactgtatct gattgaacaa ctgggtaaac
    12961 gcggcattgc ttatctgcat atgtcagaac cagattgggc gggaggtgaa ccgtatactg
    13021 atgcgttccg cgaaaaagta cgcgcccgtt tccacggtcc gattatcggc gcaggtgcat
    13081 acacggtaga aaaagccgaa acgctgattg gcaaagggtt aattgatgcg gtggcatttg
    13141 gtcgtgactg gattgcgaac ccggatctgg tcgcccgctt gcagcgcaga gctgagctta
    13201 acccacagcg tgccgaaagt ttctacggtg gcggcgcgga aggctatacc gattacccga
    13261 cgttgtaatc caacattgcg agcggcgtaa agccgccgct atactaaaac aacattttga
    13321 atctgttagc cattttgagg ataaaaagat gcgtcttctt cataccatgc tgcgcgttgg
    13381 cgatctgcaa cgctccatcg atttttatac caaagtgctg ggcatgaaac tgctgcgtac
    13441 cagcgaaaac ccggaataca aatactcact ggcgtttgtt ggctacggcc cggaaaccga
    13501 agaagcggtg attgaactga cctacaactg gggcgtggat aaatacgaac tcggcactgc
    13561 ttatggtcac atcgcgctta gcgtagataa cgccgctgaa gcgtgcgaaa aaatccgtca
    13621 aaacgggggt aacgtgaccc gtgaagcggg tccggtaaaa ggcggtacta cggttatcgc
    13681 gtttgtggaa gatccggacg gttacaaaat tgagttaatc gaagagaaag acgccggtcg
    13741 cggtctgggc aactaatctc ctgccgggcg tgaactcatc gcgcccgcat ctttactgca
    13801 tcgacaagta atatttgtca taatgcgcgc tgcaatttat ccgtattaag agaatcagat
    13861 gtccgataac gctcaactta ccggtctgtg cgaccgtttt cgtggttttt atcctgttgt
    13921 gatcgatgtt gaaacagccg gatttaacgc caaaaccgat gcgctgcttg agattgccgc
    13981 catcaccctg aaaatggatg aacaaggctg gctgatgccg gacaccacat tacatttcca
    14041 cgtcgaacca tttgtcggcg caaatttgca gccagaggcc ctcgccttca acggcattga
    14101 cccgaacgat cccgatcgcg gcgcggtcag cgaatacgag gcgctgcacg aaatttttaa
    14161 agttgtacgt aaaggtatta aagcgagcgg ctgtaaccgc gccattatgg tggcgcacaa
    14221 tgccaatttt gatcacagct ttatgatggc cgccgcagaa cgcgcctcac tgaaacgtaa
    14281 cccgttccac cctttcgcca cttttgacac tgctgcactg gccgggctgg cactcggaca
    14341 aaccgtattg tcaaaggctt gccagaccgc tggcatggac ttcgacagca cccaggcgca
    14401 ctccgcgctg tacgacaccg aacgcactgc tgtgctgttt tgtgaaatcg tcaaccgctg
    14461 gaaacgtctg ggaggctggc cgctacctgc cgccgaagag gtgtaatcga gtcgatgcct
    14521 gatgacatgc aatgattcag gcatctatag tgaggctatt ccacgcatca agcatgatat
    14581 tcacggggaa tagcgttaat ggcagataat ccagaccctt catcgctcct gccggacgtg
    14641 ttttcactgg cgacccgcga ctggtttctt cgcgccttta aacagccgac cgctgtccag
    14701 tcgcaaacct ggcatgtggc ggcgcgaggc gaacatgcgc tggtgattgc accgactggc
    14761 tccgggaaaa cgctggcagc atttctctac gccctcgatc ggctcttccg cgaaggcggc
    14821 gaagataccc gcgaggcgca taagcgtaaa acctcacgca tcctctatat ttcaccgata
    14881 aaagccctgg gcaccgacgt tcagcgcaac ttgcagatcc cgttgaaagg tattgccgat
    14941 gaacggcggc ggcgcggcga aacggaagtc aatcttcgcg tagggatccg tactggcgat
    15001 acgcctgcac aggaacgcag caaactcacc cgtaatccgc cggatattct gatcaccaca
    15061 cccgaatcac tctatctgat gctgacctcc cgcgcgcgcg aaacgctacg cggcgtcgaa
    15121 acggtaatta ttgatgaagt ccacgcggta gcgggcagta aacgtggtgc gcatctggcg
    15181 ttaagtctgg agcggctcga tgcgctgctc cacacctcag cacagcgaat tggcctttct
    15241 gccactgtgc gctcagccag cgatgtggca gcatttcttg gtggcaatcg cccggttacg
    15301 gtagtcaacc cgcccgcaat gcgccatccg cagatacgaa ttgtcgtacc ggtcgccaat
    15361 atggatgatg tctcatcggt cgccagcagc accggcgaag acagccatgc cggccgggaa
    15421 ggctccatct ggccatatat tgaaacgggt atccttgatg aagtgttgcg ccatcgctcg
    15481 accattgtct ttactaattc acgtgggctg gcggaaaaac tgacggcacg attaaatgag
    15541 ctttacgccg cacgcttaca gcgttccccg tctatcgccg ttgatgcggc ccatttcgag
    15601 tcgacctccg gcgcaacctc taaccgtgta caaagtagcg acgtttttat tgcccgctca
    15661 caccacggct ccgtctctaa agaacaacga gcaatcaccg agcaggcgct gaaatcgggt
    15721 gagttacgct gcgtggtcgc aacctccagt ctcgaactgg ggattgatat gggcgcagtg
    15781 gatctgatga ttcaggtggc aacgccgctt tctgttgcca gtgggttaca acgcattggt
    15841 cgcgccggac atcaggttgg cggtgtatct aaagggctgt ttttccctcg tacccggcgt
    15901 gacttagtcg attccgcagt cattgtagag tgtatgttcg caggcaggct ggaaaacctg
    15961 acaccgccgc ataatccact cgacgtcctt gcgcagcaaa ccgttgccgc cgcggcgatg
    16021 gatgcattac aggtagacga atggtactcc cgcgtacgcc gtgccgcacc gtggaaagat
    16081 ctgccaagac gtgtttttga cgctacgctg gatatgcttt ccgggcgcta tccctctggc
    16141 gatttttctg cttttcgccc caaactggtc tggaaccggg agaccgggat attgaccgcc
    16201 cgacctggcg ctcaattgtt ggcggttacc agcggtagca ccattccgga tcgtggcatg
    16261 tatagcgtgt tattacccga aggtgaagaa caggccggtt cgcggcgggt gggtgaactg
    16321 gatgaggaga tggtatatga gtcgcgggtg aacgacatta tcactctcgg cgctacctca
    16381 tggcggatcc agcaaatcac ccgcgatcag gtgattgtga ctcctgctcc gggtcgttct
    16441 gcccggctcc ccttctggcg tggtgaaggt aacggacgtc cggctgaatt aggcgagatg
    16501 atcggcgatt ttcttcattt gctggcggat ggcgcgttct tttccgggac tattcccccg
    16561 tggctggcag aagaaaatac gatcgccaat attcaggggt tgattgacga gcaacgcaac
    16621 gcgacgggca tcgttccggg gagtcgccat ctggtcctcg aacggtgccg tgatgaaatt
    16681 ggcgactggc gtattatttt gcactctccc tatggaagac gggtgcatga accctgggcg
    16741 ctggcgattg ccgggcgaat acatgcccta tggggcgctg acgcgtctgt ggtcgccagt
    16801 gatgacggca ttgttgcgcg cattcctgac actgatggta aattgcccga cgccgcgatt
    16861 tttttgtttg aaccagaaaa gttgctgcaa attgtccgcg aggcggtagg cagctcggca
    16921 cttttcgccg cccgttttcg cgaatgcgcc gcgcgggcat tattaatgcc ggggcgcact
    16981 ccgggccatc gcaccccgct ttggcaacaa cggctgcgcg ccagtcagtt gctggaaatc
    17041 gctcagggat atccggattt tccggtcatt ctcgaaaccc tacgcgaatg tctgcaagat
    17101 gtttatgatc ttcccgcact ggaacgtttg atgcgtcgcc tgaacggtgg cgaaattcaa
    17161 atatccgatg taacgaccac tacgccctcg cctttcgcca caagtttatt gttcggctat
    17221 gtcgcggaat ttatgtacca gagcgacgcc ccgctggcag agcgccgggc atccgtactg
    17281 tcgctggaca gcgagttact gcgcaatcta ctcggacagg tcgatccggg ggaattactc
    17341 gacccgcagg tcattcgcca ggtggaagaa gagttgcaac gactggctcc tggcagaaga
    17401 gcgaaaggtg aagaaggatt gttcgacctg ctgcgcgaac tggggccaat gaccgttgaa
    17461 gacctggcgc aacggcatac aggcagcagt gaagaggttg cgtcgtatct ggaaaatctt
    17521 cttgcagtaa aacgaatctt cccagcgatg attagcggac aggagcgtct tgcctgtatg
    17581 gatgatgccg ccaggctgcg tgatgccctc ggcgtacgac taccagagtc attgccagag
    17641 atttatttac atagagtcag ttacccgctt cgcgacctct ttctgcgcta tctccgggct
    17701 catgctctgg tcacggctga acaactggct catgagttta gtctcggtat tgccattgtc
    17761 gaagagcagc ttcagcaact gcgtgaacag ggtctggtga tgaatctgca acaagacatc
    17821 tgggtgagcg atgaagtatt tcgtcgtctg cgtttgcgct cgctgcaagc cgccagagaa
    17881 gcgacgcgtc ccgttgcagc cacgacctat gcgcgattgc tgctggaacg tcagggcgta
    17941 ttacccgcca ccgatggtag cccggcgctc tttgcctcaa catcgccagg cgtttatgag
    18001 ggcgtagatg gcgtgatgcg ggtgatcgaa cagcttgccg gagtcggttt acccgcctca
    18061 ctctgggaaa gccagatcct gcctgcccgc gtacgcgact attcgtcaga aatgctcgat
    18121 gaattactgg caaccggtgc ggttatctgg tcggggcaaa aaaactgggt gaagatgacg
    18181 gcctggtggc actgcatcta caggaatatg ctgcagaatc gttcactccc gccgaagcgg
    18241 atcaggcgaa tcgttcggcg ctgcaacaag cgatagtcgc tgttctggct gacggaggag
    18301 cctggtttgc acaacaaatc agccaacgga tacgcgacaa aatcggcgaa tcggttgatc
    18361 tctctgccct gcaagaggcg ttatgggcgc tggtctggca aggcgtcatc accagcgaca
    18421 tttgggcacc gttacgcgcc ctcacccgca gcagttccaa cgcacgcacc tcaactcgcc
    18481 gcagtcaccg ggctcgtcgt ggacgtcctg tctatgcgca acccgtctcg ccgctggtat
    18541 cttacaacac accaaatctg gctggatgct ggcgctggcg gaaaatatgc tcgaccgcta
    18601 cggcatcatc agtcgtcagg cggtgatagc cgaaaatatc cctggcgggt ttccatcgat
    18661 gcaaacgctt tgtcgaagta tggaagactc cgggcgaatt atgcaaggtc gttttgtaga
    18721 aggtctgggt ggcgcgcaat tcgctgaacg tctgactatt gaccgattgc gcgatctggc
    18781 gacacaagcc acgcaaacgc gccactatac accagtggcg ctctctgcca acgatccggc
    18841 taatgtgtgg ggaaatcttc tgccctggcc tgcacatccg gcaacgctgg ttccaacgcg
    18901 tcgggcgggt gcgctggtgg tcgtttctgg cggcaaattg ttactctatc tggcgcaagg
    18961 tggcaaaaaa atgctggtct ggcaggaaaa agaggaatta ctcgccccag aggttttcca
    19021 cgcgctgact accgcactgc gtcgcgaacc acggctgcgc tttacgctaa cagaagtgaa
    19081 tgatctaccg gtccggcaaa cgccgatgtt tacgctgctg cgtgaggcgg gattttcaag
    19141 ttcgccacaa gggctggatt ggggatagag aaaggactga cggatgcccg ttcgcatccg
    19201 tcagtattgc aggacggatt attccgcgtc cggctcttca gacttgtatt tagcggcagt
    19261 ttctttgatc agctgctgca gttcgccacg ctgatacatt tcgatcacga tatcacaacc
    19321 gccgaccagc ttgccgtcaa cccacagttg cgggaaggtc ggccagttag catatttcgg
    19381 cagttccgca cgaatgtccg gattctgcag aatatcaaca taggcaaaac gttcgccaca
    19441 tgcggcaagc gcctggactg cctgggcaga gaaaccgcag ctcggcagtt tcggtgaacc
    19501 tttcatgtac agcaggatcg ggttttcagc aatctggcgt tggatttttt cgatagtggt
    19561 gctcattgtc ttgcttcctc aaacttcttt acggcagtaa tctgacattg tagcgggtca
    19621 gtgcggcatc ggaaaataac attttcatca cgcttttgct attttatccc tttgctctat
    19681 ttgttgcatt tcaaatattc gtttcttctc tgatgccggc aaactggcgt atttataacc
    19741 atttttagct ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa
    19801 taaaagctat taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga
    19861 cgcaggcatg atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt
    19921 tcgatcacgc tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct
    19981 tcaaagcaag ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag
    20041 aaaagcacgg caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt
    20101 acgaccaaaa gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac
    20161 gccaaaactc gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag
    20221 tgtaccaaac gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga
    20281 actcttgccg acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa
    20341 ctgatgcagc aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt
    20401 gattgcagcg gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt
    20461 acggcgaatg aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa
    20521 aacggcgacc tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg
    20581 tatgtcggca acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact
    20641 tctctcagtg aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca
    20701 aaaacacttc gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt
    20761 ctcacctttt aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa
    20821 ttaataataa aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga
    20881 tgctctggca ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca
    20941 gacttatgtc actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact
    21001 ggaagagatt attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa
    21061 ccatactttc tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt
    21121 cgctgaagct atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga
    21181 tgcagcgatc aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa
    21241 actggctatc gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct
    21301 gctgaccgtt gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg
    21361 ctatctggag cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc
    21421 ataataactg atggcaaatg cagcattgcc tgatgcgctt cgcttaccat gccaacgcgg
    21481 atgaggcgtt aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca
    21541 gaaggttatc cttctgcgtt tttgtttaat tagctgttgg caacgcaaac tgtttcaggt
    21601 tgttttctgg ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgcccccatc
    21661 accggcacaa agctgtatcc cagcccagcg gaaattaccg caccaccagc cgctgctccc
    21721 agcgcatttc caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca
    21781 ctggcgacac gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc
    21841 cacaccacca tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg ggttgccagc
    21901 ataatcacca tcagcagcaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc
    21961 agtttgccgc cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc
    22021 gtgacgaaca ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag
    22081 agagtaaaca ttgcgccagc tcccagtacc gtcgtcagca atgcaggcag cacctgcgga
    22141 cgcattaata ccgccagctc ttttttcact tcaggtcgtg cccctgtacc acctttaggt
    22201 aatgagaaga acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac
    22261 atccgccagc cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc
    22321 agggttaacc ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg
    22381 cttgcggcca cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg
    22441 cgtgaaagca tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg
    22501 aaaattgcca tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc
    22561 atcagcggcg cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg
    22621 ggaatcgaga catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc
    22681 gttgtcccga taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg
    22741 ccttatctcc acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca
    22801 gcaatcacaa aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa
    22861 acaggaagga gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca
    22921 gtcaaaccca cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta
    22981 aggtcggtgc tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca
    23041 tttttacaca ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac
    23101 ttcccctttt cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat
    23161 tgaccccttc ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg
    23221 taaggtgtgt aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg
    23281 gagtgaaatg gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca
    23341 actgtgtcac acgtgatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg
    23401 tgggcagcga ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt
    23461 aaccacacca agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag
    23521 atcattgaag cagttgaaaa aaattgcttc cagaaaggtt ataccctgat tctgggcaat
    23581 gcgtggaaca atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc
    23641 gatggtctgc tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag
    23701 tatcgccata tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat
    23761 gcggtcattg ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc
    23821 ggtcaccgcg aaatcggcgt tatccccggc ccgctggaac gtaacactgg cgcaggccgc
    23881 cttgtcggtt ttatgaaggc gatggaagaa gcaatgatca aggtgccgga aagctggatt
    23941 gtgcagggtg actttgaacc tgaatccggt tatcgcgcca tgcagcaaat cctgtcgcag
    24001 ccgcatcgcc ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt
    24061 gctgctgatg aaatggggct gcgcgtcccg caggatgttt cgctaattgg ttatgataac
    24121 gtgcgcaacg cccgctattt tacgccggcg ctgaccacaa tccatcagcc aaaagattcg
    24181 ctgggtgaaa cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg
    24241 cagtccattg aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc
    24301 cgcgactatc gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc
    24361 ttattcagcg tctcactatc gccgagatac tcaagcaacc aggttaacgc aggcgacata
    24421 tcattttgct gccatgtcag acaacatgcc gaatccggaa aggggttttc cagttcaagt
    24481 gctacccact tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg
    24541 cataatcctg ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt
    24601 tggttatcca gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc
    24661 aacgacggcc agttgcgcaa cgtatcatcg ctgaacgggc catccatcaa cgccagcggg
    24721 tgatggctgg caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga
    24781 ccgcctaccg gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc
    24841 gcatcccaga caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg
    24901 cgataaaaat caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg
    24961 atagctaact gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc
    25021 atttttttga caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc
    25081 acatcacggt gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg
    25141 gtatagctga ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca
    25201 ttacgcgcta ccgcatcaac aacttcgagt gaatattctg accacatagt ctgcctgcaa
    25261 aatttttgaa accaatcatc aaatattacc gtttcacaac actaatttca ctccctacac
    25321 tttgcggcgg tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt
    25381 ttagtctggc tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct
    25441 gctttcgccg ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt
    25501 agtctgttcc ttgccggttt tgccgcagcc cagcttctgt gggggccgct ctccgaccgt
    25561 tatggtcgta aaccggtatt attaatcggt atgacaattt ttgcgttagg tagtctgggg
    25621 atgctgtggg tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt
    25681 gtctgcgccg cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa
    25741 gttaaccgaa tttttgcgac catcataccg ctggtgggtc tatctccggc cctggctcct
    25801 ctgttaggaa gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt
    25861 gccattaccg tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt
    25921 aacaatagtc aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc
    25981 aacgtgctga tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg
    26041 ttcatcctta gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa
    26101 actatcgcgt ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc
    26161 aagcagttat taccgtggtt gctggtgctg tttgctgtca gcgtcattgc gacctgggct
    26221 gcgggcttca ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg
    26281 attgccaatg gcgcgatcta ccctattgtt gtcgcccagg cgctgcgtcc cttcccacac
    26341 gcaactggtc gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca
    26401 agtctggtag tttcctggct tattagtatc agcacgccat tgctcaccac caccagcgtg
    26461 atgttatcaa cagtagtgct ggtcgcgctg ggttacatga tgcaacgttg tgaagaagct
    26521 ggctgccaga atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg
    26581 atatacttat acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat
    26641 actaatttcg agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt
    26701 cccgactcac tatggatggt catttcggca agggttcctc ctttccctct gttctacgtc
    26761 ggattataga ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg
    26821 ctgttttaag gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag
    26881 aagaagtcag tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg
    26941 ccggtatagc cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt
    27001 ttaaacgcgt tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt
    27061 gggaatgtga ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc
    27121 aactccccca tcatttcaaa gacacgctgc gtattgccgg cgctcgtctc ttcaatctgc
    27181 agagtaaaaa acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt
    27241 tcagccgcat gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata
    27301 atctggaatc tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac
    27361 ctgggatgcg cgtactggat attggctgcg gctggggcgg actggcacac tacatggcat
    27421 ctaattatga cgtaagcgtg gtgggcgtca ccatttctgc cgaacagcaa aaaatggctc
    27481 aggaacgctg tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg
    27541 accagtttga tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg
    27601 atacctattt tgcggtggtg gatcgtaatt tgaaaccgga aggcatattc ctgctccata
    27661 ctatcggttc gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc
    27721 cgaacggttg cctgccctct gtacgccaga ttgctcagtc cagcgaaccc cactttgtga
    27781 tggaagactg gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac
    27841 gattcctcgc cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacggatgt
    27901 ttacctatta tctgaatgcc tgcgcaggtg ctttccgcgc ccgtgatatt cagctctggc
    27961 aggtcgtgtt ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct
    28021 atcgccccct ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca
    28081 ttttctcgtg ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca
    28141 atttcaatgt tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt
    28201 tccggaatta aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata
    28261 ccgtcgatgc caataaatcc tttgtacagg atatatttca tcaactgact atcctggact
    28321 ttaaaccaga tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc
    28381 ataatatgac ctgacattaa gtgtccgcca atttcatcgc tgaattttgc cgcacgctca
    28441 acgtttaccc aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc
    28501 atcaggtcaa aactgacatg gttgccgtta atttccgtca ctgtcaggca gcaaccgtta
    28561 tgcgccacgg aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca
    28621 tgcgtacgaa aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc
    28681 cccgtaaaca tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca
    28741 ggtggaaaac gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta
    28801 ataaatcctc gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag
    28861 gcggcttttt agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc
    28921 agtgaagcgc gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa
    28981 actgcgatgg gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg
    29041 gcggtcgcta tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg
    29101 ctggcattaa cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg
    29161 catcaggtgc gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgctggtg
    29221 ctgtggaatg caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac
    29281 aaagccgtgg gttatctgcg tgcgttgttg tggggcgcgc caggctatct tttcttccag
    29341 gttgcccgta accagtgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt
    29401 atcggcctgc tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg
    29461 cctgagctcg gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggttatgttc
    29521 cttgccatgg tttcttacat taaacgcgcc cgctccatgc gcgatatccg taacgaaaaa
    29581 ggcaccgcaa aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt
    29641 gcgctggcac tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg
    29701 ctcggtattg ttgatgtcgc aggacaccag attgccctga actttagttc actaatgttc
    29761 gtgcttccaa tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag
    29821 ggctcaacgc tggatgcgca aaccgctgct cggaccgggc ttatggtggg tgtctgtatg
    29881 gcaaccctga cggccatttt cacggtttca ctgcgggagc aaatcgccct gttgtacaac
    29941 gacaatcccg aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag
    30001 atttctgact caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt
    30061 tccattttct atatcacctt tacagcttac tgggtgctgg gcttgccaag cggctatatt
    30121 ctggcactga ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt
    30181 attatcggcc tgacatcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt
    30241 atgccgtcag ccatcattct gcaacgagcg tcccgctaat aaagacaagg cgcaaccttc
    30301 acgggttgcg cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg
    30361 gctgtaattt cagagcgtta cacagagttt gccgactggc atctgttatc tcaacgcgta
    30421 tatccccgtt aacttcccct ttcacattga tgaagtcagg tgaaatttct ttcgcactgg
    30481 tttgcaggca aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg
    30541 ccgcaccagt gatcagccac agttttccgc cattcagatt gatgttacca acaatgaaag
    30601 tgacgctgtc accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag
    30661 taatcgttga ttgttgcagc tctgcgccat aaccaataat cattgaagcg ccgtccagta
    30721 atgcaacact cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt
    30781 ccgcgctggt gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg
    30841 taccggcatg cacttcagcc cgccccccca cattcaccga caggttttgc gtcttaccgg
    30901 aactgtagag ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag
    30961 tgtcagagtc ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg
    31021 ctttcccggc cagcataaaa acgccgccct gattaatctc acaattgctg gcttctccac
    31081 ttggatacac caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa
    31141 ccaacaggcc gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac
    31201 gcaggttacc gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt
    31261 caccttcggg atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcgcctccct
    31321 cggactgaac aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac
    31381 cgtactgtgt gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga
    31441 ccacggtatt ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg
    31501 tcgcgccaat gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt
    31561 tcgcatcaga tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa
    31621 gtgtaaaggc tacggaggat ttatccacga cagatttgag atggtggcaa acaattctgt
    31681 ttaaactctg atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa
    31741 atcgcccttg caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga
    31801 aatagcgcag aaaattacgt tttgcctctt gccacctttc cactctgccg ctaatatttg
    31861 tccccgttgt cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac
    31921 atggtggggg tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt
    31981 tggttagagc accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc
    32041 agattttctt aatctggtct tttccttttt ccctctgttt attctctgta tccaatacgt
    32101 taaaagattt acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg
    32161 ttacaacttc attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa
    32221 ccacttgctg agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt
    32281 gaaaagaacc acgaagccgg tgggatctac cttttcactg atgaaaaaag cgcccttgcc
    32341 tatctggaaa aacatactgc ccgactgaaa aatctcggcg ttgaggaagt tgtcgccaaa
    32401 gttttcgatg tcaatgagcc acttagtcaa atcaatcagg caaaactcgc ctgacagaat
    32461 ttaatcaagg gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa
    32521 tctcaaccaa acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa
    32581 tgaaaaaaat tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc
    32641 tacagcaaca aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg
    32701 ggatgccata cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg
    32761 aaataccgcc gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc
    32821 tccagcgtta tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc
    32881 tgctgggcga atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa
    32941 aatttgcagt ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg
    33001 gcgtcatgct cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg
    33061 ccacgggtca cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagcccgt
    33121 ggtcaggcct gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct
    33181 tgagcggact ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg
    33241 ataaacaaca cgtcgttttt caccgcgata acgcaagtga aaagctaaat atcacgttga
    33301 tgtcgcgcac gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct
    33361 tacacatcgt caccgatcag gcattaaatg ctgagattca aaaaggcgaa tatggccttt
    33421 tggatcgggt atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc
    33481 aacgcatagc cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc
    33541 gcaaacaacg cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata
    33601 aacgagaaaa acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac
    33661 aggaaattgt tccacatctg aatgaacacg accataaacg gttcagtaaa ggccttgccc
    33721 gggttttcat cgataattat gcggcaatcc cttcagagtc tattcgtcgg ctgctggcct
    33781 tacgtgaagc ggggatcatt catattctcg ccctcggtga agactacaaa atggaaatta
    33841 atgagtcgcg caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg
    33901 atgcccgcgg acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc
    33961 aattacagaa aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac
    34021 ccgaagatat tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc
    34081 ctttcgttca gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg
    34141 taaagcctgc atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc
    34201 actcaacaaa ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt
    34261 caccttcaat ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc
    34321 ttccccctgg cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt
    34381 caccgctggc aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa
    34441 agctggcgat ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac
    34501 aaacgcgtac tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc
    34561 gcgaagaatt accggttaca gcaacccgtg cgcgttcacc ggagctgcca atgtgactgt
    34621 taaagccgac acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc
    34681 cagcggttac gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc
    34741 gggcaaactg actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg
    34801 aaacttcctg atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca
    34861 gactctccgc ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac
    34921 taccgccgcg tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt
    34981 ctcgcaggtc tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc
    35041 ttttaaaggt ttcgtgtggc gtacgccccg tggtgcaaag ataaagatgt ccgaagataa
    35101 aaaagaggct tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag
    35161 gaaacacatc tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg
    35221 gcagcaatcc acacatgaca ccaacatagg cgacctgctg taagggatta aatttagact
    35281 gtgttgttgc cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgtg
    35341 tttgttttgc cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat
    35401 ccccaacggc attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa
    35461 cttcatgcac cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta
    35521 tcagcccact tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga
    35581 ccgctttgct gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac
    35641 gagcacgaag caaggcgtgc agccccaaca ccaaccacat cccaaccagc aatactccgg
    35701 caatgatcaa ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact
    35761 ggctctgaaa ctgttcagca tgttgcgacg ggttcataca ttttcctttt taatcaaatg
    35821 ttgaccgaac cggcgataca gatgcggttt cccggcacca agtagctgat attgatagta
    35881 tttattttcc tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc
    35941 gtgttccgga caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc
    36001 gcagaaatca catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg
    36061 acaagcgcca atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc
    36121 ctgctcatca cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca
    36181 gtgctgacat gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg
    36241 aatgtgtgct atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc
    36301 gcgggcgcaa atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac
    36361 ttccttttct tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac
    36421 tgttcccatc cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc
    36481 agtggctgca actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa
    36541 ctgctctacc tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc
    36601 gctaaacaag tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg
    36661 tgcaacatcc atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc
    36721 ttgcatcagc atggcgactt tgtaaggacg aggatacgtc tggcactcaa aacgaacggc
    36781 gtgtgcaatg gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt
    36841 ttcactcatg gcctgcgttg agtaatagta atcatcctga cttcccgtca tacaagcgat
    36901 atccgcgtag tctccgttct gcttaatgct ttccagcaaa actgacagtt cctcttctgc
    36961 aaccgaatag ggtggcgaga gaaacacggt gcgcgccacc agctgcccag ccgcagaatg
    37021 ctggcgaatg aactcagcca ggatttcagc aggttccggt tcggttaccg tatcaacggg
    37081 gtgacgcatc ccctgcaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa
    37141 cggcaattca tcctgatggt tcatcttacc cattccttac gcaggcagta gattgtgtgc
    37201 tgccagatcg gcggcaatat cttccagccc cagccgctgc aatgtttcgc gggttggaca
    37261 accaagctgt gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc
    37321 gtcacgatcc attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa
    37381 aacccaggaa cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac
    37441 cgtgtaggca cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt
    37501 catctcttcg ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta
    37561 attacggctt ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc
    37621 gttatgcaaa cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat
    37681 tggcgtgtag tttttggttt catcgtaagc atcttcagaa ccaaaaagtt ctttcgccac
    37741 ttcacgttgc agtttcaatg gcaagccgga accaataaag ttgatatggg tatgcgtcat
    37801 gcagtcacgg ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc
    37861 atggtgaacc ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc
    37921 ttcacccaaa ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc
    37981 acccacacga tgcgccagac ggtagtaaaa atctttaatt ttaatgaagt taacgtcacc
    38041 cgcttccagt tgatcccagc gaatttccgc atactcttca gcaggcagaa cacgcttgaa
    38101 cacacctttg ctgtaacaat aggtaaagtc gcgatgcaac tgcccgtagt tacaccatag
    38161 gccgtagtca tcgaacagat tcagacccac caggttacca atcacacgac catcgtcttt
    38221 atcttcaaaa tctttcggac cgttcgggaa gatggtggta tggacaaagt tggctacaca
    38281 agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat tcatttgggt
    38341 catacaacgg atcgggcaag agtggcagcc actcattttt actgtgtatt tctctgccgc
    38401 cggacctaag tcaaaaacgg atttataggt acgaaagccg accgtgttct gattgcctgg
    38461 cggaatttcc cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca gccctttacg
    38521 tgctgtccaa cgtgacttgg gatctgaata ctctgcccac gattgcggcg tacttggcac
    38581 gacatggttg ttattcgcac caataagttc agtcatcatg taatcgttga gacgcttcat
    38641 ctcctgacga tcggcaatgt tgaccccttt cgtcccttca accgcaatcg ctttcaggtt
    38701 tttcgaaccc attattgcgc cagttcccgc accgccgctg tggttacggc tattcaacat
    38761 gccagagaga ggaacaaggt tttccccagc ctgaccaata gccgccacac aggtttccgg
    38821 actggtcaat cgacaaattt cttccgtcgt tgcgcgcgtc ccttttcccc ataagaaatc
    38881 ggctttttcc aggctaactt tgtcatcttt aatcttcagc cataccggtg atttcgcctt
    38941 cccttcgata ataatgacgt cgtatccagc gaatttcatt tgcgctgcaa aaaagccacc
    39001 catatgggca tcgacgacta aatttccttt ggtaaaagta gaaagtgagg tgatatttac
    39061 gcgagaacta cagggggcac cagatccagt taatgggccg gtagcaaaga ctaatttatt
    39121 cgcttcatcg aaaggtttcg tgcctggcgg tacttcgtca tacataattt tgtagccgaa
    39181 gcccatgcca ccgacaaaac ttttaaactt actggaatct tcgagggtaa tatttcctgt
    39241 cgtgagattg actcttaata tattacctgt ccaaccgtta gccatgattt tttcctttgc
    39301 aagattcaca cagtaatatc tttccactcg ataattttta acgcccctgt tgggcaggcg
    39361 tttgcgcatt caccgcataa cacacatttc gaggattttt tactttcggt atttacggtg
    39421 gccatcatcc acgggcatgc cgtagtacag gcgctacagc caatacagcg tttatgatcg
    39481 acggtaatac agccttcttt ctgctgccag gtaatcgcac caatcgggca gacgttcatg
    39541 cattgcggct ctttgcattg acggcaggtg tccgcggtat agttgagatc gccatacagg
    39601 ccgccgccag agccaacccc gttgtcgcca aagaaataat tgcgatggat tttgatacgg
    39661 gagaagaatg tccctactga gccatcgttg aagttggtac aagagatttc acagcggtgg
    39721 caacccgtgc atcgcgctcg ctgggtcacc agcacccctt tgggtgtatt tatcaaccct
    39781 actgtgccgc tatcaatatc ttcctgtttg caacccaaaa gtgacagtaa cgcaggggca
    39841 attgttagac cagcaaggcc ttttcctgat atacgcaaga attctaatcg cgttaagcca
    39901 atatctaata gtggacgatc aaccgggttc atttatttcg catcctcttc gcaaacacag
    39961 tgctgacaat tcagcagcct gatattaagc attcaactaa tacgctgctg actctgtccc
    40021 caacaccaga tattttccct aacccgatag tgtatgagaa aagatttaac gccgataaac
    40081 caatccttta tggttaggag aaaaaataat gtgattattc tgagccctta aca
//
LOCUS       DAEEKQ010000018        39865 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.018, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000018 DAEEKQ010000000
VERSION     DAEEKQ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39865)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39865)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39865
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.018"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(118..793)
                     /locus_tag="KLK57_000960"
                     /pseudo
     CDS             complement(118..793)
                     /locus_tag="KLK57_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(938..1546)
                     /locus_tag="KLK57_000961"
     CDS             complement(938..1546)
                     /locus_tag="KLK57_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HBE0891401.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYPPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(1730..2047)
                     /gene="metJ"
                     /locus_tag="KLK57_000962"
     CDS             complement(1730..2047)
                     /gene="metJ"
                     /locus_tag="KLK57_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HBE0891402.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            2324..3484
                     /gene="metB"
                     /locus_tag="KLK57_000963"
     CDS             2324..3484
                     /gene="metB"
                     /locus_tag="KLK57_000963"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HBE0891403.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDAVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            3487..5919
                     /gene="metL"
                     /locus_tag="KLK57_000964"
     CDS             3487..5919
                     /gene="metL"
                     /locus_tag="KLK57_000964"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HBE0891404.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTDFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            6268..7158
                     /gene="metF"
                     /locus_tag="KLK57_000965"
     CDS             6268..7158
                     /gene="metF"
                     /locus_tag="KLK57_000965"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HBE0891405.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            7487..9667
                     /gene="katG"
                     /locus_tag="KLK57_000966"
     CDS             7487..9667
                     /gene="katG"
                     /locus_tag="KLK57_000966"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HBE0891406.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSERVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            9761..10666
                     /gene="yijE"
                     /locus_tag="KLK57_000967"
     CDS             9761..10666
                     /gene="yijE"
                     /locus_tag="KLK57_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HBE0891407.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVMALALVSRKKKEAVSVKRI"
     gene            complement(10693..11310)
                     /locus_tag="KLK57_000968"
     CDS             complement(10693..11310)
                     /locus_tag="KLK57_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HBE0891408.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQKRGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTHHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(11585..12688)
                     /gene="gldA"
                     /locus_tag="KLK57_000969"
     CDS             complement(11585..12688)
                     /gene="gldA"
                     /locus_tag="KLK57_000969"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HBE0891409.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGGCSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVAIAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAECE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(12699..13361)
                     /gene="fsa"
                     /locus_tag="KLK57_000970"
     CDS             complement(12699..13361)
                     /gene="fsa"
                     /locus_tag="KLK57_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBE0891410.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVQELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEHDWNAAFGTT
                     HL"
     gene            complement(13373..15874)
                     /gene="ptsP"
                     /locus_tag="KLK57_000971"
     CDS             complement(13373..15874)
                     /gene="ptsP"
                     /locus_tag="KLK57_000971"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HBE0891411.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLHTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSTLE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLRQHLLAGVSEGLSCAEAIVAS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLWALDYAVQTVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            16183..17262
                     /locus_tag="KLK57_000972"
     CDS             16183..17262
                     /locus_tag="KLK57_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HBE0891412.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGTWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            17277..17597
                     /locus_tag="KLK57_000973"
                     /pseudo
     CDS             17277..17597
                     /locus_tag="KLK57_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
     gene            17648..19944
                     /locus_tag="KLK57_000974"
                     /pseudo
     CDS             17648..19944
                     /locus_tag="KLK57_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
     gene            19910..20788
                     /locus_tag="KLK57_000975"
     CDS             19910..20788
                     /locus_tag="KLK57_000975"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HBE0891413.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            20790..21131
                     /locus_tag="KLK57_000976"
     CDS             20790..21131
                     /locus_tag="KLK57_000976"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HBE0891414.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(21118..21969)
                     /locus_tag="KLK57_000977"
     CDS             complement(21118..21969)
                     /locus_tag="KLK57_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0891415.1"
                     /translation="MYHGVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(22184..23917)
                     /locus_tag="KLK57_000978"
     CDS             complement(22184..23917)
                     /locus_tag="KLK57_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HBE0891416.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(24100..26751)
                     /gene="ppc"
                     /locus_tag="KLK57_000979"
     CDS             complement(24100..26751)
                     /gene="ppc"
                     /locus_tag="KLK57_000979"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HBE0891417.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(27103..28254)
                     /gene="argE"
                     /locus_tag="KLK57_000980"
     CDS             complement(27103..28254)
                     /gene="argE"
                     /locus_tag="KLK57_000980"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HBE0891418.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASIGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDTLRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPSMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            28408..29412
                     /gene="argC"
                     /locus_tag="KLK57_000981"
     CDS             28408..29412
                     /gene="argC"
                     /locus_tag="KLK57_000981"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HBE0891419.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            29420..30196
                     /gene="argB"
                     /locus_tag="KLK57_000982"
     CDS             29420..30196
                     /gene="argB"
                     /locus_tag="KLK57_000982"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HBE0891420.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSTLVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            30257..31630
                     /gene="argH"
                     /locus_tag="KLK57_000983"
     CDS             30257..31630
                     /gene="argH"
                     /locus_tag="KLK57_000983"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HBE0891421.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALSVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIGEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLG"
     gene            31897..32814
                     /gene="oxyR"
                     /locus_tag="KLK57_000984"
     CDS             31897..32814
                     /gene="oxyR"
                     /locus_tag="KLK57_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HBE0891422.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGYFDKVLKQAV"
     gene            complement(32797..34197)
                     /gene="sthA"
                     /locus_tag="KLK57_000985"
     CDS             complement(32797..34197)
                     /gene="sthA"
                     /locus_tag="KLK57_000985"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HBE0891423.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGVRVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            34518..35168
                     /gene="fabR"
                     /locus_tag="KLK57_000986"
     CDS             34518..35168
                     /gene="fabR"
                     /locus_tag="KLK57_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HBE0891424.1"
                     /translation="MMGIRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            35168..35527
                     /locus_tag="KLK57_000987"
     CDS             35168..35527
                     /locus_tag="KLK57_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HBE0891425.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(35567..36667)
                     /gene="trmA"
                     /locus_tag="KLK57_000988"
     CDS             complement(35567..36667)
                     /gene="trmA"
                     /locus_tag="KLK57_000988"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HBE0891426.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPCILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      36781..36971
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            37036..38880
                     /gene="btuB"
                     /locus_tag="KLK57_000989"
     CDS             37036..38880
                     /gene="btuB"
                     /locus_tag="KLK57_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HBE0891427.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRGTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            38825..39682
                     /gene="murI"
                     /locus_tag="KLK57_000990"
     CDS             38825..39682
                     /gene="murI"
                     /locus_tag="KLK57_000990"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HBE0891428.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         9094 a        10028 c        11003 g         9740 t
ORIGIN      
        1 tcatttgaga aaaaagagca gaatgaggta aaaatctttg gctgtgtatt cagtctggta
       61 atcatccaag gaaagcttat cagaagggtg atgactccaa ttagtagaac atatattttc
      121 gataaaagca ccgatgactt tttcatgaag ttctacagaa cgcgagaaac agatggttct
      181 gcgggccaga cgtttgattc gggttctcag ggtcagattg ttacgctcaa tgtgctgagt
      241 aaaaatctta ccagtcagat gcttctcctt cggtaactct ctagcgtaac taccccattc
      301 gtcagtggtc aacatgccga tggtaaaagg gtaagcagcg cccgcaattc acgacaggtt
      361 tcgtcagtcc gggggccaaa agtgtaggcc agaacaccac cagttttcgt gttgtacacg
      421 taccagagcc agtgctgtcg cgctttactg ccgacaaagc tccattgctg actaagtttg
      481 cagatgagtg ccacatcagc atgagcgacc ggggatgacg ttattcttcg tggcgagagt
      541 tttttaaagt tcggatgact gtattaatgc cgattttcag cgtacgggca gtatcgcgaa
      601 ccccggcacc gttgaaagtc atttcagcga tttggtcttt aactcccggt ttacggggct
      661 cgtaacagta actgagctga aatacccggt gacagtcacg gccgcggaat cgctcatgtc
      721 tttttgggtt ctgcccgtga cggtaaacct gaacggactg gcaacggggg caatgaaggt
      781 taactgtgac catgaaacaa ccttaaaagc gagtattata tccttaattc aactaattgg
      841 aggcatcacc gtttaccgga gcgacagctt gcggaaaggt cgtgcaacta caaagcaaca
      901 aacagactac ggcgggaatt atttttttca tcatccatta ccacgaaaac agcggccact
      961 ctttggcgac tgtggtgagt tgtggcgcgt cgaaaacggc ttgtggtgaa actaccgttt
     1021 cctcaagcgg aatatttttg ccgtagcgtt ctttgagctt agcctggacc agcgggttgc
     1081 tgagatcgaa ctctttcaac ttctgctgct cgcccacgcg cattcccagc gcgttctggt
     1141 aaaccttcca caccacttcc gaacagtact ggcgatcgtc gctccatgag aagctgaaat
     1201 catacggttt accgagataa cgttttgccg tttgcgccag tttttgctgt tgttcaacac
     1261 tcagtccgcc ttcaacgcgg cgaacaacgt atttgccctt ttcaccatgg gcgatccact
     1321 gcttgagcgg ggggtatttc accgggccga ccgcttcaaa aacgtagggc tttttgttgc
     1381 gtatcaccag cataccggtg tggctataat cggaatgggt cgccagttgg atcgctttac
     1441 tttgcgatga gcgagagatc tgaaagatga tgtcgccggt ttgtggctgc caggcaaagg
     1501 caggtacaga aaccagcagg ctgaggatca gcagcctgtt tttcatagtt aaacgtccat
     1561 gtataaaaag cggtgggtcg cagacaacgt gctcgttgtt tatgccggat gcggcgtgaa
     1621 cgccttatcc ggcctacaag ttcgtgcaaa ttcaataaat tgcaatatga cgtaggcctg
     1681 ataagcgtag cgcatcaggc gattccactc cgcgccgctc ttttttgctt tagtattccc
     1741 acgtctccgg gttaatcccc atctcacgca tgatctcttt tgccgcttcc gggatttcgt
     1801 cgctgcgctc tttacgcaga tcggcatcat ccggcaaagg ttgcccggta aaggcatgca
     1861 gaaacgcttc gcacagcagc tcgctgttgg tagcgtgacg caggttgttc acctgacgac
     1921 gcgtgcgttc atcggtgagg atttttaaca ccttaagagg aatggaaacc gtaatctttt
     1981 tgacttgttc actcttcttg ccgtgctcag cgtatgggct gatatattcg ccgctccatt
     2041 cagccatgag atacttaatc ctcttcgtca ataaattgag accagaccac acaatttatg
     2101 tgggtactga ccgtaaaccc gcatagttta ccgtacaggc gttaccgtga catcgtgtaa
     2161 tgcacctgtc ggcgtgataa tgcatataat tttaacggct atttgggatt tgctcaatct
     2221 atacgcaaag aagtttagat gtccagatgt attgacgtcc attaacacaa tgtttactct
     2281 ggtgcctgac atttcaccga caaagcccag gaaacttcat cacatgacgc gtaaacaggc
     2341 caccatcgca gtgcgtagcg ggttaaatga cgacgaacag tatggttgcg ttgtccctcc
     2401 catccatctt tccagcacct ataactttac cggatttaat gaaccgcgcg cgcatgatta
     2461 ctcgcgtcgc ggcaacccaa cgcgcgatgt ggttcagcgt gcgctggcag aactggaagg
     2521 cggtgctggc gcagtactta ctaataccgg catgtccgcg attcacctgg taacgaccgt
     2581 ctttttgaaa cctggcgatc tgctggttgc gccgcacgac tgctacggcg gtagctatcg
     2641 cctgttcgac agtctggcga aacgcggttg ctatcgcgtg ttgtttgttg atcaaggcga
     2701 tgaacaggca ttacgggcag cgctggcaga aaaacccaaa ctggtactgg tagaaagccc
     2761 aagtaatcca ttgttacgcg tcgtggatat tgcgaaaatc tgccatctgg caagggaagt
     2821 cggggcggtg agcgtggtgg ataacacctt cttaagcccg gcattacaaa atccgctggc
     2881 attaggtgcc gatctggtgt tgcattcatg cacgaaatat ctgaacggtc actctgacgc
     2941 ggtggctggc gtggtgattg ctaaagaccc ggacgttgtc actgaacttg cctggtgggc
     3001 aaacaatatt ggcgtgacgg gcggcgcgtt tgacagctat ctgctgctac gcgggttgcg
     3061 aacgctggtg ccgcgtatgg agctggcgca gcgcaacgcg caggcgattg tgaaatatct
     3121 gcaaacccag ccgttggtga aaaaactgta tcacccgtcg ctgccggaaa atcaggggca
     3181 tgaaattgcc gcgcgtcagc aaaaaggctt tggcgcaatg ttgagttttg aactggatgg
     3241 cgatgagcag acgctgcgtc gtttcctgag cgggctgtcg ttgtttacgc tggcggaatc
     3301 attaggggga gtggaaagtt taatctctca cgccgcaacc atgacacatg caggcatggc
     3361 accagaagcg cgtgctgccg ccgggatctc cgagacgctg ctgcgtatct ccaccggtat
     3421 tgaagatggc gaagatttaa ttgccgacct ggaaaatggc ttccgggctg caaacaaggg
     3481 gtaaaaatga gtgtgattgc gcaggcaggg gcgaaaggtc gtcagctgca taaatttggt
     3541 ggcagtagtc tggctgatgt gaagtgttat ttgcgtgtcg cgggcattat ggcggagtac
     3601 tctcagcctg acgatatgat ggtggtttcc gccgccggta gcaccactaa ccagttgatt
     3661 aactggttga aactaagcca gaccgatcgt ctctctgcgc atcaggttca acaaacgctg
     3721 cgtcgctatc agtgcgatct gattagcggt ctgctacccg ctgaagaagc cgatagcctc
     3781 attagcgctt ttgtcagcga tctggaacgc ctggcggcgc tgctcgacag cggtattaac
     3841 gacgcagtgt atgcggaagt ggtgggccac ggggaagtat ggtcggcacg cctgatgtct
     3901 gcggtactta atcaacaagg gctgccagcg gcctggcttg atgcccgcga gtttttacgc
     3961 gctgaacgcg ccgcacaacc gcaggttgat gaagggcttt cttacccgtt gctgcaacag
     4021 ctactggtgc aacatccggg caaacgtctg gtggtgaccg ggtttatcag ccgtaacaac
     4081 gccggtgaaa cggtgctgct ggggcgtaac ggttccgact attccgcgac acaaatcggt
     4141 gcgctggcgg gtgtttctcg tgtaaccatc tggagcgacg tcgccggggt atacagtgcc
     4201 gacccgcgta aagtgaaaga tgcctgtctg ctgccgttgc tgcgtctgga tgaagccagc
     4261 gaactggctc gtctggcggc tcccgttctt cacgcccgta ctttacagcc ggtttctggc
     4321 agcgaaatcg acctgcaact gcgctgtagc tacacgccgg atcaaggttc cacgcgcatt
     4381 gaacgcgtgc tggcctccgg tactggtgcg cgtattgtca ccagccacga tgatgtctgt
     4441 ttgattgagt ttcaggtgcc cgccagtcag gatttcaaac tggcgcataa agagatcgac
     4501 caaatcctga aacgcgcgca ggtacgcccg ctggcggttg gcgtacataa cgatcgccag
     4561 ttgctgcaat tttgctacac ctcagaagtg gccgacagtg cgctgaaaat cctcgacgaa
     4621 gcggggttac ctggcgaact gcgcctgcgt caggggctgg cgctggtggc gatggtcggt
     4681 gcaggcgtca cccgtaaccc gctgcattgc caccgcttct ggcagcaact gaaaggccag
     4741 ccggtcgaat ttacctggca gtccgatgac ggcatcagcc tggtggcagt actgcgcacc
     4801 ggcccgaccg aaagcctgat tcaggggctg catcagtccg tcttccgcgc agaaaaacgc
     4861 atcggcctgg tattgttcgg taagggcaat atcggttccc gttggctgga actgttcgcc
     4921 cgtgagcaga gcacgctttc ggcacgtacc gactttgaat ttgtgctggc aggcgtggtg
     4981 gacagccgcc gcagtctgtt gagctatgac gggctggacg ccagccgcgc gttagccttc
     5041 ttcaacgatg aagcggttga gcaggatgaa gagtcgttgt tcctgtggat gcgcgcccat
     5101 ccgtatgatg atttagtggt gctggacgtt accgccagcc agcagcttgc tgatcagtat
     5161 cttgatttcg ccagccacgg tttccacgtc atcagcgcca acaaactggc gggagccagc
     5221 gacagcaata aatatcgcca gatccacgac gccttcgaaa aaaccgggcg tcactggctg
     5281 tacaatgcca ccgtcggtgc gggcttaccg atcaaccaca ccgtgcgcga tctgatcgac
     5341 agcggcgata ctattttgtc gatcagcggg atcttctccg gcacgctctc ctggttgttc
     5401 ctgcaattcg acggtagcgt gccgtttacc gagctggtgg atcaggcgtg gcagcagggc
     5461 ttaaccgaac ctgacccgcg tgacgatctc tctggcaaag acgtgatgcg caagctggtg
     5521 attctggcgc gtgaagcagg ttacaacatc gaaccggatc aggtacgcgt ggagtcgctg
     5581 gtgcctgccc attgcgaagg cggcagcatc gaccatttct ttgaaaatgg cgatgaactt
     5641 aacgagcaga tggtgcaacg gctggaagcg gcccgcgaaa tggggctggt gctgcgctac
     5701 gtggcgcgtt tcgatgccaa tggcaaagcg cgtgtgggcg tggaagcggt gcgtgaagac
     5761 catccgctgg catcactgct gccgtgcgat aacgtctttg ccatcgaaag ccgctggtat
     5821 cgcgataatc cgctggtgat ccgtgggcct ggcgctgggc gcgacgtcac cgccggggcg
     5881 attcagtcgg atatcaaccg gctggcacag ttgttgtaat ttcagaaatt taataatgcc
     5941 cggtactcat gttttcgggt ttatggtttc taatgaaata tattgaatta tcataggatt
     6001 aggccggatt aagcgtttac gacgaatccg gcaagaagca ataagtacat ggttagttta
     6061 tatttgcagt ccggtttgct ttgcataccg gattttcttt ttcttaccat cctgaagttt
     6121 tttcatcttc cctgattttt cctcaccatc attggtcatt tttcggttga cgcccttcgg
     6181 cttttccttc atctttacat ctggacgtct aaacggatag atgtgcacaa cacaacatat
     6241 aactacaagc gattgatgag gtaaggtatg agcttttttc acgccagcca gcgggatgcc
     6301 ctgaatcaga gcctggcaga agtccagggg cagattaacg tttcgttcga gtttttcccg
     6361 ccgcgtacca gtgaaatgga gcagaccctg tggaactcca tcgatcgcct tagcagcctg
     6421 aaaccgaagt ttgtatcggt gacctatggc gcgaactccg gcgagcgcga ccgtacgcac
     6481 agcattatta aaggcattaa agatcgcact ggtctggaag cggcaccgca tcttacttgc
     6541 attgatgcga cgcccgacga gttgcgcacc attgcacgcg actactggaa taacggtatt
     6601 cgtcatatcg tggcgctgcg tggcgatctg ccgccgggaa gtggtaagcc agaaatgtat
     6661 gcttctgacc tggtgacgct attaaaagaa gtggcagatt tcgatatctc cgtggcggcg
     6721 tatccggaag ttcacccgga agcaaaaagc gctcaggcgg atttgcttaa tctgaaacgc
     6781 aaagtcgatg ccggagccaa ccgcgcgatt actcagttct tcttcgatgt cgaaagctac
     6841 ctgcgttttc gtgaccgctg tgtatcggcg ggcattgatg tggaaattat tccgggaatt
     6901 ttgccggtat ctaactttaa acaggcgaag aaatttgccg atatgaccaa cgtgcgtatt
     6961 ccggcgtgga tggcgcaaat gttcgacggc ctggatgatg atgccgaaac ccgcaaactg
     7021 gtgggcgcga atattgccat ggatatggtg aagattttaa gccgtgaagg agtgaaagat
     7081 ttccacttct atacgcttaa ccgcgctgaa atgagttacg cgatttgcca tacgctgggt
     7141 gttcgacctg gtttataaat agtgtggctt ttgtgaaaat cacacagtga tcacaaattt
     7201 taaacagagc acaaaatgct gcctcgaaat gagggcggga aaataaggtt gtcagccttg
     7261 ttttctccct cattacttga aggatatgaa gctaaaaccc ttttttataa agcatttgtc
     7321 cgaattcgga cataatcaaa aaagcttaat tacgatcaat ttgacctaca tctctttaac
     7381 caacaatatg taagatctca actatcgcat ccgtggatta attcaattat aacttctctc
     7441 taacgctgtg tatcgtaacg gtaacactgt agaggggagc acattgatga gcacgtcaga
     7501 cgatatccat aacacgacag ccactggcaa atgcccgttc catcagggcg gtcacgacca
     7561 gagcgcgggg gcgggcacaa ccactcgcga ctggtggcca aatcaactcc gtgttgacct
     7621 gttaaaccaa cattctaatc gttctaaccc gctgggtgag gactttgact accgcaaaga
     7681 attcagcaaa ttagattact acggcctgaa aaaagatctg aaagccctgc tgacagaatc
     7741 tcagccgtgg tggccagccg actggggcag ctacgccggt ctgtttattc gtatggcctg
     7801 gcacggcgca ggtacttacc gttcaatcga tggacgcggc ggcgcgggtc gtggtcagca
     7861 acgttttgca ccgctgaact cctggccgga taacgtaagc ctcgataaag cgcgtcgcct
     7921 gttgtggcca atcaaacaga aatatggtca gaaaatctcc tgggccgacc tgtttatcct
     7981 cgcaggtaac gtggcgctgg aaaactccgg cttccgtacc ttcggttttg gtgccggtcg
     8041 tgaagacgtc tgggaaccgg atctggatgt taactggggt gatgaaaaag cctggctgac
     8101 tcaccgtcat ccggaagcgc tggcgaaagc accgctgggt gcaaccgaga tgggcctgat
     8161 ttacgttaac ccggaaggcc cggatcacag cggcgaaccg ctttctgcgg cagcagctat
     8221 ccgcgcgacc ttcggcaaca tgggcatgaa cgacgaagaa accgtggcgc tgattgcggg
     8281 tggtcatacg ctgggtaaaa cccacggtgc cggtccgaca tcaaacgtag gtcctgatcc
     8341 agaagctgca ccgattgaag aacaaggttt aggctgggcg agcacttacg gcagcggcgt
     8401 tggcgcagat gccattacct ctggtctgga agttgtctgg acccagacgc cgacccagtg
     8461 gagcaactat ttcttcgaaa acctgttcaa atatgagtgg gtacagaccc gcagtccggc
     8521 tggcgcaatc cagttcgaag cggtagacgc accggaaatt atcccggatc cattcgatcc
     8581 gtcgaagaaa cgtaaaccga cgatgctggt gaccgacctg acgctgcgtt ttgatcctga
     8641 gttcgagaag atctctcgtc gtttcctcaa cgatccgcag gcgttcaacg aagcctttgc
     8701 ccgtgcctgg ttcaaactga cgcacaggga tatggggccg aaatctcgct acatcgggcc
     8761 ggaagtgccg aaagaagatc tgatctggca agatccgctg ccgcagccga tctacaaccc
     8821 gaccgagcag gacattatcg atctgaaatt cgcgattgcg gattctggtc tgtctgttag
     8881 tgagcgggta tcggtggcct gggcatctgc ttctaccttc cgtggtggcg acaaacgcgg
     8941 tggtgccaac ggtgcgcgtc tggcattaat gccgcagcgc gactgggatg tgaacgccgc
     9001 agccgttcgt gctctgcctg ttctggagaa aatccagaaa gagtctggta aagcctcgct
     9061 ggcggatatc atcgtgctgg ctggtgtggt tggtgttgag aaagccgcaa gcgccgcagg
     9121 tttgagcatt catgtaccgt ttgcgccggg ccgcgttgat gcgcgtcagg atcagactga
     9181 cattgagatg ttcgagctgc tggagccaat tgctgacggt ttccgtaact atcgcgctcg
     9241 tctggatgtt tccaccaccg agtcactgct gatcgacaaa gcccagcaac tgaccctgac
     9301 cgcgccggaa atgaccgcgc tggtgggcgg gatgcgcgta ctgggtgcca acttcgatgg
     9361 cagcaaaaac ggcgtcttca ctgaccgcgt tggcgtattg agcaatgact tcttcgtgaa
     9421 cttgctggat atgcgttacg agtggaaagc gaccgacgaa tcgaaagagc tgtttgaagg
     9481 ccgtgaccgc gaaaccggcg aagtgaaata cactgccagc cgtgcggatc tggtatttgg
     9541 ttctaactct gtcctgcgtg cggtggctga agtttacgcc agcagcgatg ctcacgagaa
     9601 gtttgttaaa gacttcgtgg cggcatgggt gaaagtgatg aacctcgacc gtttcgacct
     9661 gctgtaatct gaccttcttc agcgactgcc tttcaggcag tcgctgaagt ttctttaccg
     9721 gcgtatagtg tccacaggaa aactacacac tggatctctc atgtctgccg caggaaagag
     9781 caacccactg gcaatcagtg gcctggttgt gctcacactt atctggagtt atagctggat
     9841 tttcatgaag caagtcacca gctacatcgg tgccttcgac tttaccgcct tacgctgcat
     9901 tttcggcgct ctcgttttat tcatcgtcct tttattacgt ggtcgcggaa tgcgcccgac
     9961 accgtttaaa tacaccttag ccattgccct gttacaaacc tgcgggatgg ttggcctggc
    10021 gcagtgggcg ctggtcagcg gcggagcggg gaaggtggcg atcctaagct ataccatgcc
    10081 gttctgggtg gtgattttcg ccgcgttgtt tctcggtgaa cgcctgcgac gtgggcaata
    10141 tttcgcgata ctgattgccg ctttcggctt atttttggtg ttgcagccgt ggcaactcga
    10201 tttctcttcg atgaaaagtg ccatgctggc aatcctctcc ggcgtcagtt ggggggcgag
    10261 cgcgattgtt gctaaacgtc tgtatgcccg tcatccgcgc gtggatttat tgtcgttaac
    10321 atcctggcag atgctctatg cggcgctggt gatgagtgtg gtcgctttac tggtgccgca
    10381 acgtgaaatt gactggcagc ccaccgtgtt ctgggcgctg gcctacagtg cgattctggc
    10441 gacggcactg gcgtggagct tatggttgtt tgtattgaaa aacttgcctg ccagtattgc
    10501 cagcttaagc acactggccg ttcccgtttg cggcgtactc ttttcctggt ggctgctcgg
    10561 cgagaatccg ggggccgttg aaggtagcgg tattgtgctg attgtgatgg cactggcgct
    10621 ggtgagccgt aagaaaaaag aagccgttag tgtaaaaagg atctgaattt tttcttcatg
    10681 tggggcgatc gcttatttaa caaaataacg ataatgcccc accatgcgcc agctaaacag
    10741 cacatcttct tcctgcgcac ctgcgccaat gttatgtatc accagcggcg taccgtcgcg
    10801 ggcgaagcca tctgaaacca ccccaatatg tgccagcccg ttatctaatc gccaggagac
    10861 aatatcgccc gcttgatagt cactggggtt cttgctgatg gggcgtgttt tatcgtggtg
    10921 ggtaaaccag gtttccagat taggcacccg acggtgatcg atgttgctgt ccgggcgctt
    10981 taactgccat ttttgcgggt actcggcaaa attcttcgcc atatcttcgt gcaccagttt
    11041 ctgcaaatcg accttctggc tgcgcaatgc acggatcacc acatcggaac ataccccgcg
    11101 tttttgcggg acatcaccgc caggataggt aagctgcata tacgccggat cgtaaaatag
    11161 cgtgctgccg atttgctgtc tggcaccgtc tgcgatggca aggttggtat tggcctggat
    11221 ttgtaccacg gttggtggaa cggcgggaga ttttaaggag tggctggtaa atgccgttag
    11281 caggctgagc agcgccagtg aagctttcat ctggttcgtc cgtgaaaata agagtcacag
    11341 attcgacctt ccgggataac gattgttctg tagagaaaat gtccagaagc ggataaaaaa
    11401 ctcctgaaca acgtgggagt ttcagattac tggcacagag tgccggatgc ggcgtgaacg
    11461 ccttatctgt cctaaaaaaa aaagaaaatt caatatattg cagagattgc gtaggcctga
    11521 taagcgtagc gcatcaggca attttgcgtt caaactcccg gacaagccgg gagtttggag
    11581 taggttattc ccactcttgc aggaaacgct gaccgtactg gtcggctacc agcagagcgg
    11641 cgtaaacctg atctggcgtc gcgccgccag gcatgttgtg gatggtttca cattctgcac
    11701 atgccgcttc agccacaatt cgcattttcg ccgggacatc ttctttaata tccagttgag
    11761 cgagagttat tggcaaacct accgcatggc taagtgcagc tacggtttcg atttcctcaa
    11821 ccggtgcgtt ttccagaacc agctgcgtca gcgtaccaaa tgccactttt tcaccgtgat
    11881 aatagtgatg cgcgtccggg atagcggtca ggccgttatg cactgcgtgc gccgcagcca
    11941 gaccaccact ttcaaaacca acaccgctca aataggtgtt cgcttcaatc acgcgctcca
    12001 gcgccggagt cactacatgc tgttcggcag caagcatcgc tttttcgcct tcttccagca
    12061 gggtgttgta gcacagttca gccagtgcca gcgcagcctg ggtgcacttg ccgcccgcca
    12121 tggtggtcgc gccgctacga gagcaggcac gcgcttcaaa ccaggttgcc agcgcatcgc
    12181 cgatacccgc cgctaacaga cgtgcaggtg cgccagcgac gattttggtg tcgacaatga
    12241 ccatattcgg gttatttggc aacagcagat agcggtcaaa ctcaccctca tcggtgtaga
    12301 taacagacaa tgcgctgcac ggtgcatcgg tagaggcgat agtcggtgcg atcgctaccg
    12361 gaacacccat gaaatgtgcc agtgctttgg ctgtatcgag ggtttttccg ccaccgatac
    12421 cgagaattgc gccacactgc gcagtctccg cgatgccacg cagacggtcg atctcatttt
    12481 gcgaacatcc accgccaaac ggcgcaattt ctactaccag tccagcatct ttaaagcttt
    12541 tctcgacagt ggattgagca aaacctaaaa caaatttgtc acccaccact aaccagcgtt
    12601 ctgccagcgg cttcaggtat tcgcccagac gattaatcac atcagcgccc tggatgtatt
    12661 tacccggtga ttgaataatg cggtccataa ttgctccttt agagatgagt agtgccaaat
    12721 gcggcattcc agtcgtgttc gaacttctct atagctgact ctaccgcagg ggtgttgagc
    12781 atttgttgcg ctacatctaa gggcagggtg atggattcac atcctgccag taaacagtcc
    12841 agcgcctgac gcggcgtttt aaagctggct gccagcacca tgctttctgg cgcgtgcatt
    12901 tctaacagcg cttgcagctc ctgaaccgta cgaatgccgt ctccgccctg ggcatctacg
    12961 cggttaacat acggagcaac gtattttgcc cctgccagcg cggccagtaa cccttgtgca
    13021 gcactgtaca ctgcggtgcc aagcgtagta ataccctctt ttttcagtat tttaattgct
    13081 gccagacctt cggaagtcac cgggattttc accacaatac ccggaatagc gtcgcgcagg
    13141 cgcttcgctt cttccaccat cccctgcgcg tcgcggctca tggtctgagc aaacagaatg
    13201 ccctcatcac caatcgcttt ttgcagacgc ggcagcactt cccatatgga ctccttgctg
    13261 gcagcgataa tgctcgggtt agttgtcacc ccggcaatgg ggaatatgcg tgccagacgt
    13321 tcgacttctg cgacgttagc ggtgtccaga tacagttcca tgatgtttcc ttttacagtt
    13381 ccagttcatg ttgcagcagg ctggcgatag cgtcggcaga ggcggcgtta accagcgcgt
    13441 tacggaattc ttcgtgcatg atgcggcgag cgaggcgcga gaaaatgcgc atgtgctgat
    13501 cgcccgcagc gtgtttgttc agggttaaca tgatgatgaa ttgcgcttca tcatcacccc
    13561 agcgcaccgg cgcttgcaga cgcgcaacgc tgatggtgga ttgctcaatg tgttctgatt
    13621 tgctgtgtgg aatggcaaaa ctaaagccca gaccggtaga gaaaacggcc tcgcgcgccc
    13681 acaagtcggc ttccagttta cgcggatagc ggcagcgccc tgccagcagc aggttatcgg
    13741 tcatgccttt gagcacttct tctttgctgc gccagtcgct ttccagtgtg atgcactcgg
    13801 cggtgaccag cggtgcgtct tgttgggtca tgcggaattg cgccagcagg tgctccactt
    13861 ccagcgaagt acggcaggcc attgcctggt tgagcaactt gcggcactca cggctatcaa
    13921 gttgcgccat ccgcgctttc gccgccggaa ttgatggtgc gctcatgctg agttcatcca
    13981 gccctaagcc gaccagcaac ggcagcacgg aacctttcgc tcccagctca ccgcacagac
    14041 caatccattt accctggcga tgcaccgtct gcacggcgta atcgagcgcc cacaagaatg
    14101 ccggattcag gctgttgtag tgacgagtaa ccttagcgtt atcgcgatcc actgccagca
    14161 gatattgcgt caggtcatta ctaccaatac taaagaaatc aatctcttcg cagcattgat
    14221 cgatgatgaa catcaccgac ggcacttcca gcataatgcc gagctggatc ttctcatcaa
    14281 acggaatgtg ttcgttacgt agttgctgtt tggcttctgc cagtttttct ttcacccata
    14341 agatctcttc cattgaggag atcatcggga tcatgatttt caggctgccg tgagcggagg
    14401 cgcgcaggat cgagcgtagt tgtgtggtaa ataacgacgc gtattcttca taaatacgca
    14461 cggcgcgata accgaggaac gggtttgcct ctgcgggaat gtttagataa tcaacgggtt
    14521 tgtcgccgcc aatgtccata gtgcgcacaa taatgctgcg tccgttggca gactccagcg
    14581 cctgacaaaa aatgttgtac agctcgcttt cgcccggtgc actggtgcga tccatataga
    14641 gcatttcagt gcggaacaaa ccaacgcctt ccgcaccatt gccgaatgcg gcctgcgctt
    14701 ccacggagtg agcgatgtta gcggcaattt caatgcggat accgtcagcg gtacgggctt
    14761 gttgggtcag ccagacacgc tgttgctcac gcagggcgtc ctgtacgcgg gcttcttgct
    14821 gataataacg agctacggct tctcctggct caaccacaat cgccccggcg ttgccgtcga
    14881 tataaatcgt ttgatgctgc cacggagtga gggcatcaat atccacacca accagcgtcg
    14941 gaatgttgaa cgaacgggca aggatcaccg tatgtgaggt ggtgccgcca cttttcagca
    15001 acagaccttt gaggtgattt ttatccagtt cgaggaactg gctgggggtc agttcatcag
    15061 ccatacaaat ggcggactgc gtcagtttgc ccggtgccgg gaagcgttgc tcaccgtaga
    15121 tttgctggag taactggaag cagacgtcgc gtacgtccag ggcacgttct tgcaggtagc
    15181 tgctgctgga acgggcgaac tcttcgcaaa agtgattcgc actggcgaca atcgcttcgg
    15241 cgcagcttaa tccctcgctg acgcctgcca gtaagtgctg acgtaaagat gtatcgccag
    15301 ccagggatcg gtgagcttcc agtatcgcgc tggtagcacc gtcgctatcc agcagacgaa
    15361 actcaatgtt tttcagtacc agcgtcaggc cgttttccag tgtggattgc tcggcgtcaa
    15421 cgtctttggc tgcgggaaga ttactcagcg cattgagatc taaagaagag atcggcgtca
    15481 gaatgccgcc tgcactaccg ctgcacacgg tgcgggcgcg gataatttgc ggattcagat
    15541 tggtcagtga aaccggcagt ggttccagtt cgtcagattt aacttccgcc agcggcgcat
    15601 cgcagtgggg gaattcatcg cgcagccatt ggcttaaacg ctggtgcgct tcctgttcgt
    15661 cggccccgga aatcagtaac tggcagttat cgcccgccag cgtatcggtg ccaatcagcg
    15721 caagggcgct tttggcgttg cccttgcggt cagtgtgcag gttatgccac tcaatttgtg
    15781 atgaaaaagt attacacagc gtttcaacgt ggcttgccgg acgcgcatgt acgccgttag
    15841 gtagctcaca aataaattcc acaatcaggg ccattgcctc tctcccggta cggttttctg
    15901 tttacaggat aagagcgcac ggcaacggcc tgccatgtga caaatctgcc aaaagctgga
    15961 caaatgtaat gtaaccgtca atttgcgacg cgtctcacaa gacgccgttt tgcggcatgc
    16021 ttccggttta tcgcaagtta tgaggcggat cgcatttttg tactgatatt acaaaagtcc
    16081 agtaaatggc ctttttatcc actgtttgct ccgcctgcga ttgcctattg tttcctccat
    16141 caaaaatatg gacatgggct ttcgcaagcc cgggagcagc ttatgaatga gttggtgcag
    16201 atcctgaaaa atacccgtca gcatttaatg acgggcgttt cacacatgat tcccttcgtg
    16261 gtatcgggcg gtattttgct ggcggtttcc gtcatgttgt atggcaaagg cgcagtgccg
    16321 gatgccgtag ccgatccaaa tctgaaaaaa ctgtttgata tcggcgttgc gggcttgacg
    16381 ctgatggtgc ctttcctcgc cgcttacatt ggttattcca ttgcagaacg ttctgcgctg
    16441 gctccatgcg ctatcggtac ctgggttggt aacagctttg gtgcgggctt ctttggtgca
    16501 ctgatcgccg ggattatcgg cggcatcgtg gtgcattacc tgaagaaaat tccggtgcat
    16561 aaagttctgc gctcggtgat gcctatcttc attattccta tcgtcggcac actgattacc
    16621 gcaggcatca tgatgtgggg cttgggcgag cctgtagggg cgttgaccaa cagcctgact
    16681 cagtggcttc aggggatgca gcagggcagc attgttatgc tggcggtgat catgggtctg
    16741 atgctggcgt tcgatatggg cggtccggtt aacaaagtgg cctatgcctt catgctgatt
    16801 tgcgttgctc agggtgttta taccgtggtg gccatcgctg ccgtgggtat ttgtatcccg
    16861 ccgctgggga tggggctggc gacgctgatt ggtcgtaaaa atttctccgc agaagagcgc
    16921 gaaaccggca aagcggcact ggtgatgggc tgtgttggcg ttactgaagg ggcgattcct
    16981 ttcgccgctg ccgatccgct gcgtgtcatt ccttccatca tggtcggttc agtttgtggt
    17041 gcagtaactg cggcgctggt cggtgcgcag tgctatgcag gctggggtgg tctgattgtg
    17101 ctgccggtgg ttgaaggcaa gctgggttat atcgcagcag tggctgtcgg agcagtggtg
    17161 acggctgttt gtgtgaacgt gctgaaaagt ctggcgcgta aaaatgggtc ttcgactgat
    17221 gaaaaagaag acgacctgga tttggatttt gaaattaatt aattgaggaa cgagccatga
    17281 cgaaaattat tgcagtaacc gcatgtcctt caggtgttgc ccatacctat atggcagcag
    17341 aggcgctgga aagtgccgcg aaagcgaaag gctgggaagt gaaagtagaa acctaggggt
    17401 caattggtct ggaaaatgaa ctgactgcgg aagatgtggc gagcgctgat atggtgattc
    17461 tgaccaaaga tatcggcatc aagtttgaag agcgttttgc tggcaagacc atcgtgcgcg
    17521 tcaacatcag cgatgcagta aaacgtgctg acgccattat gagcaaaatt gaagctcacc
    17581 tggcgcaaac cgcttaagtt cttctcccgc tcgcaagggc gggttcgctt tcccacagga
    17641 gttcctcatg acgaatcgta tctctcgcct caaaactgca ctgtttgcca atacccgtga
    17701 aatctcgctg gagcgggcgc tgctttatac cgccagccat cggcaaaccg aaggcgaacc
    17761 ggtgatattg cgccgggcga aagcaacagc gtatatcctt gaacatgttg aaatttcgat
    17821 tcgtgatgaa gaactgattg ccggtaaccg caccgtaaaa ccgcgcgccg ggattatgtc
    17881 gccggaaatg gacccttact ggctgctgaa agagctggat caattcccga cgcgtccgca
    17941 ggaccgcttt gctatcagcg aagaagataa acgtatctac cgtgaagagt tgttcccgta
    18001 ctgggaaaaa cgttcgatga aagatttcat caacgggcag atgacagatg aagtaaaagc
    18061 cgcgaccaac acgcagattt tcagcatcaa ccagacggat aaaggccagg ggcacattat
    18121 tattgattac ccacgcctgt tgaatcacgg tctgggtgag ctggtggcac agatgcagca
    18181 acattgtcag caacagccgg agaatcactt ttatcaggcc gcgttgttac tgctggaagc
    18241 ctcgcagaaa cacattttgc gttacgccga actggcggaa acgatggcgg caaactgcac
    18301 agatgcacag cgtcgcgaag aactgctgac tattgcggag atctctcgcc ataacgccga
    18361 acataagccg cagacgttct ggcaggcgtg ccagttattc tggtacatga acatcattct
    18421 gcaatacgaa tccaacgcca gttcgctgtc tttagggcgc ttcgaccagt atatgttgcc
    18481 gttctatcag gcatctttaa cccagggcga agatccggcg ttcctgaaag aactgctcga
    18541 atctttatgg gtgaaatgca acgacatcgt gctgttgcgc tccaccagca gcgcgcgtta
    18601 tttcgcaggt ttcccgaccg gctataccgc actgctcggc gggttaaccg agaacggacg
    18661 tagcgcggtg aacgtgcttt cgttcctttg ccttgacgcc tatcaaagcg tgcaattacc
    18721 gcaaccgaac ctcggcgtgc gcactaacgc cttgatcgac acgccgttcc tgatgaaaac
    18781 cgccgaaacc attcgcctcg gcaccggtat tccgcaaatc tttaacgatg aagtggtggt
    18841 accagcgttc ctcaaccgtg gcgtttcgct ggaagatgcg cgcgactatt ccgtagtggg
    18901 ctgtgtggaa ttatctattc ccggcagaac ctacggcttg catgacatcg cgatgtttaa
    18961 tctgctgaaa gtgatggaaa tctgcctgca tgaaaatgaa ggcaatgctg cgctgactta
    19021 tgaaggttta ctggagcaga tccgcgccaa gatcagccac tacatcaccc tgatggttga
    19081 gggcagcaat atttgtgata tcggccatcg cgactgggca cctgtaccgc tgctctcgtc
    19141 ttttatcagc gattgtctgg aaaaaggtcg cgatattacc gatggcggcg cgcgttataa
    19201 cttctccggc gtacagggga tcggtatcgc caacctgagc gattctctcc atgcgttgaa
    19261 agggatggtt tttgagcaac agcgtttaag ttttgacgaa ttgctgtcgg tattaaaagc
    19321 caactttgca acgccagaag gcgaaaaagt ccgtgctcgc ttaattaacc gcttcgagaa
    19381 atacggtaac gatatcgacg aggtggataa catcagcgcc gaactgttac gccactactg
    19441 caaagaagtg gaaaaatacc agaacccgcg cgcggttact tcacgccggg atcgtatacc
    19501 gtttctgctc acgtcccgtt gggatcggtg gttggcgcga cgccagacgg tcgttttgcc
    19561 ggggaacaac tggcagacgg cggcttgtca cctatgctgg gccaggacgc acaagggcca
    19621 acagcggtac tgaagtcagt cagtaagctc gataatacgc tgctgtctaa cggtacgttg
    19681 ctgaacgtga aattcactcc ggcgaccctg gaaggtgaag cgggattacg caaactggcc
    19741 gacttcttac gggcgtttac ccagcttaag ttgcagcata ttcagtttaa cgtggtgaac
    19801 gccgacacgt tgcgggaagc gcaacagcgc ccacaagatt atgccgggct ggtggtgcgc
    19861 gttgccggat acagcgcctt ctttgtcgaa ctgtcgaagg agatccagga tgacatcatc
    19921 cgccggacag cgcatcagct gtaacgttgt ggaaacgcgc cgcgatgatg tggcgcgcat
    19981 tttcaacatt cagcgttatt cactgaatga cggcgagggc attcgtacgg tggtcttttt
    20041 taaaggctgt ccgcatcttt gcccgtggtg tgctaatccg gagtcgatct ccggcaaaat
    20101 ccagacggta cgcagagagg cgaaatgtct gcactgtgcg aaatgtttgc gtgatgcgga
    20161 tgaatgcccc tccggggcgt ttgaacggat tggtcgcgat atcagccttg acgctctgga
    20221 acgggaagtg atgaaagatg acattttctt tcgcacgtcc ggcggcggcg tcacgctttc
    20281 tggcggcgaa gtgttaatgc aggcggagtt tgctacccgt tttttacagc gactgcggct
    20341 gtggggtgtg tcatgcgcca ttgaaactgc cggagacgcg ccagcaagca agctgttacc
    20401 gctggcgaaa ttgtgcgatg aagtgctgtt cgatttaaaa attatggacg cgactcaggc
    20461 gcgggatgtg gtgaagatga acctgccacg cgtgctggag aatctgcgtt tgctggtgag
    20521 tgagggtgtc aacgtgatcc cgcgtttacc gttgatccct ggtttcacgc tcagcaggga
    20581 gaatatgcag caggcgctgg atgtactgat cccgctgaaa atcaggcaga tccatctgtt
    20641 accgtttcat cagtacggcg aaccgaaata ccgcctgctg gggaaaacat ggtcgatgaa
    20701 agaggtgcct gcgccgtcgt cagccgatgt gacaacgatg cgcgaaatgg cagaacgggc
    20761 cggatttcag gttaccgtgg gaggttaaaa tggcatacct ggtggcagta accgcctgcg
    20821 taagcggcgt ggcgcatact tatatggcgg cggaacggct ggaaaagttg tgccagttag
    20881 agaagtgggg cgtcagcatt gaaactcagg gcgcgctggg aacggagaat cgtttagcgg
    20941 acgaggatat ccgtcgggcg gatgttgctc tgttgattac ggatatcgag cttgccggtg
    21001 ccgagcgatt tgaacattgc cgctatgtgc aatgcagcat ctacgcattc ctgcgtgagc
    21061 cgcagcgggt aatgagcgcg gtgcgcaaag tgctttctgc gccgcagcaa acccatctta
    21121 ttctggagta gtcggttttt ctgtcagttg gctgtggtac tgccggcgat attccgacgg
    21181 cgagcgttcg gtgtttttac gaaacagacg gcagaagtag ttgctgtcga caaagccgca
    21241 ggcgtgcgcc acttctttta ccttcaggtc atagcctttc aataacgtct tagcgtgctc
    21301 cagtcgcgtg tgattcaggt attcgttaaa accaatggcc cccgtttttt gaaacaggtg
    21361 cgagagatag tttggtgaaa tatagaatgc ctgtgcgaca gattcgcggg taagcgcgga
    21421 ggcatagcgt tcgtcgatat aatcgcgaat agcttcaaat agtgcctggc tgcgtgaggc
    21481 ggtctggatt tggctgccga gcaaatcgcg gcagtggcta agcaggctgg ctacgataag
    21541 ccttgccgtt tgctgctcct gcggctgcat ttgcatttca ttgagcgttt gtagcagaaa
    21601 agaacctata cgtgggccgc gtcgggcgac gtgttgcttc gccagatttt gatattgttt
    21661 gccatcccat tgcacgacgc tgaagccgag ttgctgtttg ccaaacagca cgctaaaggt
    21721 ggtagcgggt gttttccatt gtgggaaatt ccagccacca gcggcaacgt acagcacatc
    21781 gccgggaact aacgttgctc cagcgccggt atcaacaaac tcaccttcca gcacaatttc
    21841 cagccgtgga aaatcgacct gatacgcaag atcgggcacc gggccgttac tgctggcaaa
    21901 gtaaatctgg cgcagggaca gcgggccgtt gatcaggcgg gagagcagat agctgacgcc
    21961 gtgatacatg ttattccctt aggtatgtag acgggaaaac agtaactggc attattaaaa
    22021 aaggccacct gaaaagtgtg gcctgagggg agttcgatgt aaacattgcg gcagttgtat
    22081 tgccggatgt ggcgtaaacg ccttatccag cctaccgccg cgatctgtag gccggataag
    22141 acgcgtcaag cgtcgcatcc ggcaaatagc gcctggctga taattactga ttacctacct
    22201 ggtcgccata cggcagagtg tcgtaatcga tcagcgcgtt tttcttgtac gggttaccaa
    22261 tccagcgggt agtttctttg aactgcggat tcaccactga acgggttgga tcgtaaccgt
    22321 cgtaagataa gcccgccaaa tctgaccagg tgtggatcag ttccgccagg ctgtatttac
    22381 gatcgacatc ctgtgagaaa tcacggggat gagtcgcttg ccatttttcc gacgtccaca
    22441 gcaggaacgg aatggtgtac atgtggcgcg tcgggttgtc ttcgttacgc ccctgagttt
    22501 tgtgcggcgg cgtgtcgtaa acctcttcac cgtggtcaga gaagtaaacc aggaatccgt
    22561 tcgggtctgc cgctttaaag tctttaatca ggctggcaac cacatgatcg ttatacaggt
    22621 tagcgttgtc ataatcgtta tatgattcca gctcttctgc gcttaatcct ggcggaacat
    22681 gatcggtatt gccatcaaac ttgccctgat tttccgggta gcggtatttg tatttgatat
    22741 gcgtacccag cagatgaacg atgatcagtt tcttcggcgc agggtcattc agcacatcct
    22801 ggaacggctt cagcacgttg gtgtcgtatt cacgcgcact ctgcgtacgt tgctggttca
    22861 tgtagtactg cttgtcggtc tggcgcgaaa ataccgtcag catggtattg cgggcggtca
    22921 tcgtctgctg gttggtgatc cagaaggttt tataacccgc ctgtttcatc atgttcatca
    22981 gcgacggctg cgtcagatac agatccgggt tcttttcatt ggcaaaggtc agcgcctgtt
    23041 gcaggatttc aatggtgtac ggacgagacg taactacgtt attaaacacg gtcagattcg
    23101 gatcggtttt atgcagcgca tccagctccg gcgtggtttc acgcggataa ccgtacagac
    23161 tcatgcgtcc gcgctgggtc gactcgccaa tcaccagcac taaagtgcgc ggttcgttac
    23221 ccgattcatc tttgaaatta gccagtggcg gcaaggcatt attttcattc agtaactttg
    23281 ttagcgagtt tagttgctga cgatactgat aatagccggt caggaattgc cacggtgcgg
    23341 caggctccat acgcgaggcc aggttatcca acgttttctc aaacggcttg tttttgataa
    23401 acgtattcat ggcgatcgga tgcagaatca agccataaag cagggcaaaa gagacaacat
    23461 aacgccacgg ctttggaata tagaccgggc gcaggcgtgt ccacagcaga actgccaccg
    23521 ccgtataggc cagcgcgata agcacaattt tcaggctgaa atactggctt aaatactcgc
    23581 tggcttcgtt ggtgttggtt tcgaacatca caaacagaac gctctgcgag aactcctgcc
    23641 cgtagatgac gtagtagcat agcgccgcca gagaggccgc ccatagcacc acgccgatta
    23701 ctgcggcaat aattttaatc cgcttcggaa agaggaatac cgggatcaac cacagcgagc
    23761 tgaataacag cgagtcgcga atgccgttgg tgccactata accactgatg taaataatgg
    23821 cctgtagcag agtagagaaa aaccaaaagt agagcagtgc ccaacccagg gctttccagc
    23881 taaaaagggg tttagcctgg acttctgtgg aatgcatagt aagaacctgt cttgaaaaaa
    23941 atatcgccga atgtaacgac aattccttaa ggatatctga aggtatattc agaatttgaa
    24001 taaaatgcag acagaaatat attgaaaacg agggtgttag aacagaagta tttcagaaaa
    24061 ccctcgcgca aaagcacgag ggtttgcaga agaggaagat tagccggtat tacgcatacc
    24121 tgccgcaatc ccggcaatag tgaccattaa cgcttgttcg acgcgaggat ccggttcctg
    24181 gccttctttt tctgcctggc gggagcggtg cagcaactcg gcctgcaata cgttcagcgg
    24241 gtcggtgtaa atattccgta gctgaataga ctctgcaatc cacggcagat cggccatcag
    24301 atgggaatcg ttggcaatcg ccagcaccac tttgatgtct tcttcttgca ggttgcgtaa
    24361 ctctttacct aacggccaca gtgctttgtc taccagacgt tgatcatagt attccgccaa
    24421 ccacaggtct gctttggcga agaccatctc cagcatgccg agacgcgtcg agaagaatgg
    24481 ccaatcgcgg cacatggctt ccagctcgct ctgtttgcca tcttcgacca ctttttgcag
    24541 cgccgtacct gcacccagcc aggcggggag catcaggcgg ttttgcgtcc aggcaaaaat
    24601 ccacggtatg gcgcgcagcg actcgacgcc gccggttggg cgacgtttcg ccggacgtga
    24661 acccaacggc agtttgccca attcttgttc cggcgtagcg gagcggaagt aaggcacaaa
    24721 atctttgttt tcacgtacgt agccacggta gagatcgcag gagatgactg acagttcatc
    24781 cataatgcga cgccagctct ctttcggctc aggtggtggc agcaggttgg cttccagaat
    24841 cgccccggtg taaagcgaca ggctgctgac ggtgatttct ggcagaccat atttaaagcg
    24901 gatcatctcg ccctgttcag tcacgcgcag gccacctttc aggcttcctg gcggttgtga
    24961 cagcagcgcc gcatgggcag gtgcgccgcc gcgaccaatg gaaccgccgc gaccgtggaa
    25021 caacgtcagc tcaatacccg ctttttcgca ggttttgatt aatgcatcct gtgcctgata
    25081 ttgcgcccag gaagctgcca tcacgcccgc atcttttgct gagtcggaat agccaatcat
    25141 caccatctgt ttgccctgaa tcaggccgcg ataccagtcg atattgagca gctgggtcat
    25201 gacatcgttg gcgttgttca ggtcatcgag ggtttcaaac agcggagcaa ccggcatcgc
    25261 aaacccgata cccgcttctt tcagcagcag gtggacagcc agtacgtcgg acggcgtttt
    25321 cgccatcgag atcacgtagg cggcaatgga gccttgcggc gcttcggcaa tcacctggca
    25381 ggtatcgagc acttcgcgcg tttcggcgct tggttgccag ttgcgcggca gaagcggacg
    25441 tttggagttc agttcgcgga tcaggaacgc ctgtttgtcg gcctctgacc agctttcgta
    25501 gtcgccgata ccgaggtagc gggtcagctc gcccagcgct tcggtatggc gggtgctctc
    25561 ctgacggata tcaatacgga ccagcggtac gccgaaacat ttcacgcggc gcagggtgtc
    25621 gagcagatcg ccgttggcga taatacccat gccacacgcc tgaagtgact ggtagcaagc
    25681 gtagagcggt tcccacagtt cttcgttttg tgtcagcagc ccttctggtt ttggcagttc
    25741 ttcgcctttc aggcgcgctt ccagccatgc ctgtgtcgcc atcaggcgag aacgcaggtt
    25801 tttcatcaga tagcgatacg gttctgcggc accttcttcg ccaaccagcg ccagcagttc
    25861 aggggtcgct tcaaccatcg acagttcaga aaccagaacc tgaatatctt tcaggaacag
    25921 atcggtggct ttccagcgac tgagtagcag gacgtggcgg gtgatatcgg cagtgacgtt
    25981 cgggttgccg tcgcggtcac cgcccatcca cgaagtaaaa cggaccggaa caaattcgac
    26041 gggcagtttg tagccgaggt tctcttccag ttgttcgttc agttcgcgca agtaattcgg
    26101 tacgccttgc cacaggctgt tttctactac ggcaaagccc catttggctt catctaccgg
    26161 gcttggacgc agcttacgga tttcatcagt atgccatgac tgggcgatta actggcgcag
    26221 gcgacgcatc agctggttgt gttcgtagtc agcgatatct ttgttatcga gctgttttaa
    26281 gcaggcgttc acttccacca ttttgtggat cagtgtacga cgggtaattt cggttgggtg
    26341 agccgtgagg accagttcca gcgacagcga ttccactgct tttttgatgg tgtcttcgct
    26401 cagttccggc tggtttttca gtttgcgcag ggtgcgggcg atcacttccg ggttgctggc
    26461 agcttcgcct ttcggcgaaa tgctgtggta ttgctcggcg gtgttggcca ggttcaggaa
    26521 ctgactaaac gcacgcgcaa cgggcagcag ctcgtcgttc gacaaatttt gtaaggtggt
    26581 gagcaactcc tggcggttag catcattgcc agcgcgtgaa gatttcgaca acttacggat
    26641 agtttctacg cgttcaagaa tgtgttctcc caacgcatcc ttgatggttt ctcccagcac
    26701 tttgccgagc atactgacat tactacgcaa tgcggaatat tgttcgttca tattacccca
    26761 gacaccccat cttatcgttt gatagccctg tatccttcac gtcgcattgg cgcgaatatg
    26821 ctcgggcttt gcttttcgtc gtcttttata aagccacgta aaagcggtga cgtcaaatgc
    26881 tgcgaaatcg cttcagcaaa cgaataaata gcaggaattt acgccattaa attcacgacg
    26941 ctttaaataa gcgtaactta tggaaatgtt aaaaaatcgc catacataac accaaaggtg
    27001 taggtcggat aagatgcgca agcatcgcat ccgacattat tgcggcaccg gagtttggca
    27061 acggtgccgg atgcggcgcg agcgccttat ccggcctacg ttttaatgcc agcaaaaatg
    27121 gtgaattacc tggataatca actcacgggt gggtttaata aaccgcgtct ccagatattc
    27181 atcaggctga tgagcttgat tgatggaacc cggaccgagc accagcgtcg ggcataacgt
    27241 ttgaataaac ggcgcttcgg tacagtaatt caccacttcg gtttttgctc cgagcaattt
    27301 ctcaaccact tcaaccagtt gatgattcgg tgggcattca tagccaggga tcggcggatg
    27361 cagctcgtcg accgtcagac gacccggcca gcgttcgctc accggagcca atgcatcgtt
    27421 gagcaaacca ttaagttcat tgagtgtcat gctaggcagc ggacgaatat ccatatgcaa
    27481 ctcacagcaa gcgcaaatac ggttagaagc gtcgccaccg tgaatatgcc cgaggttgag
    27541 cgtagggtat ggcacggtaa atgcttcgta gtgataacgt tctttcaggt tatcgcgcaa
    27601 ttgcaaaata tgcccgatgg cgtcgtgcat tagttcgata gcgttaactc cgcgtgctgg
    27661 atcgctggag tgccccgact ggccctgaat acggatggcg ttagagatat gacctttatg
    27721 tgcgcgtacc ggttgtagtg acgtcggttc gccaatgatg gcgcaatccg ggcgcagggc
    27781 ggtagtttcg gcaaaataac gcgctccggc catactggtt tcttcatcag cagtcgccag
    27841 aatgtagagc ggttttttca gtttcgtgac gtcgacatcg cgtagcgtat caaggataaa
    27901 cgcaaaaaag cctttcatgt cggcggtgcc taagccgtaa agcttgccgt catgctccgt
    27961 cagtgtaaac ggatcgcgcg tccagcgacc gtcatcaaat ggcaccgtat cggtatgccc
    28021 cgccagcaac aagccgccag ccccctgtcc gatacttgcc agcatattga atttgttgcg
    28081 agttcctggc acaggctgca cttccacatt gaagcctaaa tctttaaacc agtccgccag
    28141 cagagtgatt aaatctgcat tgctttgatc gagtgcctct tccgtggcgc ttattgaagg
    28201 tgtggcaatc agagcacggt aaatctcgat aaatggcggt aatttgtttt tcattgttga
    28261 cacacctctg gtcatgatag tatcaatatt catgcagtat ttatgaataa aaatacacta
    28321 acgttgagcg taataaaacc caccagccgt aaggtgaatg ttttacgttt aacctggcaa
    28381 ccagacataa gaaggtgaat agccccgatg ttgaatacgc tgattgtggg tgccagcggc
    28441 tacgctggcg cagagctagt gacctatgta aatcgccatc cgcatatgaa cataaccgct
    28501 ttgactgttt cagcgcaaag caatgatgcg ggaaagttaa tctccgattt gcatccgcag
    28561 ctaaaaggca tcgttgagct gccgttgcag ccgatgtcgg atatcagcga gtttagccca
    28621 ggggtggacg tagtgtttct cgccaccgcc catgaagtta gccacgattt agcgccgcag
    28681 tttcttgaag cgggctgcgt ggtgttcgac ctttccggtg cgtttcgtgt taacgacgcc
    28741 accttctatg aaaaatatta cggctttacc catcaatacc cggaactgct ggaacaggca
    28801 gcctacggtc tggcggagtg gtgcggtaat aaattaaaag aagcgaattt gattgcggtg
    28861 ccaggctgtt atccgacggc ggcacagctg gcgctgaaac cgttgattga tgccgatctt
    28921 cttgacctca atcagtggcc ggtgatcaac gccaccagcg gcgtgagcgg tgcaggacgt
    28981 aaggcagcga tttcaaatag tttttgtgaa gttagcctgc aaccgtatgg cgtctttact
    29041 catcgccatc aaccagagat cgccacacac ctcggtgctg acgttatctt caccccacat
    29101 ctgggcaatt tcccgcgcgg cattctcgaa accattacct gccgcctgaa atcgggtgtg
    29161 acccaggcgc aagtcgcgca agtgttacag caggcgtatg cccataaacc gctggtgcgg
    29221 ctgtatgaca aaggcgttcc ggcgctgaaa aatgtcgttg ggctgccatt ttgcgatatc
    29281 gggtttgccg ttcagggcga gcatctgatt attgtggcga ccgaagacaa cttactgaaa
    29341 ggcgcggcgg cacaagcggt acagtgcgcc aatattcgtt tcggctatgc ggaaacgcag
    29401 tctcttattt aagggtgcaa tgatgaatcc attaattatc aaactgggcg gcgtactgct
    29461 ggatagtgaa gaggcgctgg aacgtctgtt tagcacactg gtgaattatc gtgagtcaca
    29521 tcagcgtccg ctggtgattg tgcacggcgg cggttgcgtg gtggatgagc tgatgaaagg
    29581 gctgaatctg ccggtgaaaa agaaaaacgg cctgcgggtg acgcctgctg atcagataga
    29641 cattatcacc ggagcactgg cgggaacggc aaataaaacc ctgttggcat gggcgaagaa
    29701 acatcagatt gcggccgtag gtttgtttct cggtgacggc gacagcgtca aagtgaccca
    29761 gcttgatgaa gagttaggtc atgttggact ggcgcagcca ggttcgccta agcttatcaa
    29821 ctccttgctg gagaacggtt atctgccggt ggtcagctcc attggcgtaa cagacgaagg
    29881 gcaactgatg aacgtcaatg ccgaccaggc ggcaacggcg ctggcggcaa cgctgggcgc
    29941 ggatctgatt ttgctctccg acgtcagcgg cattctcgac ggcaaagggc aacgcattgc
    30001 cgaaatgacc gccgcgaaag cagaacaact gattgagcag ggcattatta ctgacggcat
    30061 gatagtgaaa gtgaacgcgg cgctggatgc ggcccgcacg ctgggccgtc cggtagatat
    30121 cgcctcctgg cgtcatgcgg agcagcttcc ggcactgttt aacggtatgc cgatgggtac
    30181 gcggatttta gcttaagttt tgttggccgg aggcgcagct ttccggcatt gaatttcaaa
    30241 ataaggaaac agagttatgg cactttgggg cgggcgtttt acccaggcag cagatcaacg
    30301 gttcaaacaa ttcaacgact cactgcgctt tgattaccgt ctggcggagc aggatattgt
    30361 tggctctgtg gcctggtcca aagccctggt cacggtaggc gtgttaaccg cagaagagca
    30421 ggcgcaactg gaagaggcgc tgagcgtgct gctggaagat gttcgcgcca ggccacaaca
    30481 aatccttgaa agcgacgccg aagatatcca tagctgggtg gaaggcaaac tgatcgacaa
    30541 agtgggccag ttaggcaaaa agctgcatac cgggcgtagt cgtaacgatc aggtagcgac
    30601 tgacctgaaa ctgtggtgca aagataccgt tagcgagtta ctgacggcta accgacagct
    30661 gcaatcggcg ctggtggaaa ccgcacaaaa caatcaggac gcggtcatgc caggttacac
    30721 tcacctgcaa cgcgcccagc cggtgacgtt cgcgcactgg tgcctggcct atgttgagat
    30781 gctggcgcgt gatgaaagcc gtttgcagga tgcgcttaag cgtctggatg tcagcccatt
    30841 aggctgtggc gcgctggcgg gaacggccta tgaaatcgac cgtgaacagt tagcaggctg
    30901 gctgggcttt gcttcggcga cccgtaacag tctggacagc gtttctgacc gtgaccatgt
    30961 gttggaactg ctgtcggctg ccgctatcgg catggtgcat ctgtcgcgtt ttgctgaaga
    31021 tctgattttc tttaacaccg gcgaagcggg gtttgtggag ctttctgacc gcgtgacttc
    31081 cggttcatca ttaatgccgc agaagaaaaa cccggatgcg ctggagctga ttcgcggtaa
    31141 atgcggtcgg gtgcagggtg cgttaaccgg catgatgatg acgctgaaag gtttgccgct
    31201 ggcttacaac aaagatatgc aggaagacaa agaaggtctg ttcgacgcgc tcgatacctg
    31261 gctggactgc ctgcatatgg cggcgctggt gctggacggc attcaggtga aacgtccacg
    31321 ttgccaggaa gcggctcagc agggttacgc caacgccacc gaactggcgg attacctggt
    31381 ggcgaaaggc gtacctttcc gcgaggcgca ccatatcgtg ggtgaagcgg tggtggaagc
    31441 cattcgtcag ggcaaaccgc tggaagatct gccgctcgac gagttgcaga aattcagccc
    31501 ggtgattggc gaagatgtct atccgatttt gtcgctgcaa tcgtgcctcg acaagcgtgc
    31561 ggcaaaaggc ggcgtctcac cgcagcaggt ggcccaagcg attgcttttg cgcaggcgcg
    31621 gttggggtaa gaacatttat atgtacaaat ttgagcctgg cttatcgccg ggctttttta
    31681 tggcaaaaaa aagcggatcc tggagatccg caaaagttca cgttggcttt agttattcga
    31741 gttgagaaac tctcgaaacg ggcagtgact tcaagggtta aaagaggtgc cgctccgttt
    31801 ctgtgagcaa ttatcagtca gaatgcttga tagggataat cgttcattgc tattctacct
    31861 atcgccatga actatcgtgg cgatggagga tggataatga atattcgtga tcttgagtac
    31921 ctggtggcat tggctgaaca ccgccatttt cggcgtgcgg cagattcctg ccacgttagc
    31981 cagccgacgc ttagcgggca aattcgtaag ctggaagatg agctgggcgt gatgttgctg
    32041 gagcggacca gccgtaaagt gttgttcacc caggcgggaa tgctgctggt ggatcaggcg
    32101 cgtaccgtgc tgcgtgaggt gaaagtcctt aaagagatgg caagccagca gggcgagacg
    32161 atgtccggac cgctgcatat tggtttgatt cccacggttg gaccgtacct gctaccgcat
    32221 attatcccga tgctgcacca gacctttcca aagctggaaa tgtatctgca tgaagcacag
    32281 acccaccagt tactggcgca actggacagt ggcaaactcg attgcgtgat cctcgcgctg
    32341 gtgaaagaga gcgaagcatt cattgaagtg ccgttgtttg atgagccaat gttgctggct
    32401 atctatgaag atcacccgtg ggcgaaccgc gaatgcgtac cgatggccga tctggcaggg
    32461 gaaaaactgc tgatgctgga agatggtcac tgtttgcgcg atcaggcaat gggtttctgt
    32521 tttgaagccg gggcggatga agatacacac ttccgcgcga ccagcctgga gacgctgcgc
    32581 aacatggtgg cggcaggtag cgggatcact ttactgccag cgctggctgt gccgccggag
    32641 cgcaaacgcg atggggttgt ttatctgccg tgcattaagc cggaaccacg ccgcactatt
    32701 ggcctggttt atcgtcctgg ctcaccgctg cgcagccgct atgagcagct ggcagaggcc
    32761 atccgagcaa gaatggatgg ctatttcgat aaagtgttaa aacaggcggt ttaaaccgtt
    32821 taacgcagct acccgatagg cttccgccat ggtcgggtag ttaaaggtgg tgttgacgaa
    32881 gtactcaata gtgttgccgc cacctttctg ttccataatc gcctgaccga tatgaataat
    32941 ttcggcagcg cgctcgccaa agcagtgaat acccagaatc tcttttgttt cccgatggaa
    33001 caaaattttc agcgtgccca cgttcatgcc gacgatttgt gcgcgtgcca gatgtttaaa
    33061 ctgggcgcgg cccacttcat atggcacttt catcgcggtc agctgctgtt cggttttgcc
    33121 cacagagctg atttccggga tggtgtaaat gccggtaggg atatcttcaa tcagatgcgc
    33181 ggtggcttcg ccttttacca gcgcctgcgc ggcaatgcgc ccctggtcat aggccgctga
    33241 tgccaggctc ggataaccaa tcacgtcgcc caccgcgtaa acgtgcggct gtgcggtctg
    33301 atacatgctg ttgaccttca gctgtccgcg actgtcagtt tccagcccaa tgttctgtaa
    33361 cgccagcgaa tcggtattac cggtacgacc gttggcatag agcaggcagt cagctttcag
    33421 ttttttaccc gacttcagat gcatgatcac accgtcgtca cagccttcga tcttctcgta
    33481 ctcttcgttg tgacgaatca ctacgccact gttccagaag tgataggaga gagaatctga
    33541 catctcttga tcgagaaatg ccagcaggcg atcgcgggtg ttgatcagat ccacttttac
    33601 atccataccg cggaagatcg acgcatattc acagccgatc actccagcac catagataag
    33661 tacatggcgc ggttcgtggt gcatgctaag aattgagtcg ctgtcgtaaa tgcgtggatg
    33721 ggtgaaatca acatctgttg gatgatatgg acgagagccg caggcaataa caaatttttc
    33781 agcggttagt gtttcgacgc tgccgtccgg gcaatccagc gccaacgtat gctcgtcaac
    33841 aaagcgagcg ttcccctgca atatttcaca gtgattacgt tcgtaaaatc cctgacgcat
    33901 gcgcgtttgt tgattaatca cgttatcggc atggttaagg atatcggcaa aagaagagcg
    33961 gagcagtcgc gaatggtcgc tgtatagtgg gttttgattg aattctataa tgcggctgac
    34021 ggcgtgacgg agagctttcg acgggatggt gccccagtgg gtgcaaccgc cgccaacatt
    34081 ttgataacgc tcgataactg cgacgcgcac accttgctta accaggccca ttgcagcgcc
    34141 ttcgccgccg gggccggaac ctattactat ggcatcgtaa tcgtaggaat gtggcatggt
    34201 agggcttacc tgttcttata cataaaagca acagaatggt aacattctat cgcgggtaag
    34261 ccaattgatc cccgtcattt atctggctat atcctgagcg gcctttgctt tgtctgtttc
    34321 ttacttttgc cctgacgttt tattggattt ttatcgacga tactctccgt ttaagcggca
    34381 ggtttccgct gtacgtaaaa gaaccggcca gtaaatatgt tttattgcgt taccgttcat
    34441 tcacaatact ggagcaatcc agtatgttca ttctctggta tagtgccagc agtacttttg
    34501 gcaaggattc agacatcgtg atgggcataa gagcgcaaca aaaagaaaaa acccgccgtt
    34561 cgctggtgga agccgcattt agccaattaa gtgctgaacg cagcttcgcc agcctgagtt
    34621 tgcgtgaagt ggcgcgtgaa gcgggcattg ctcccacctc tttttatcgg catttccgcg
    34681 acgtagacga actgggtctg accatggttg atgagagcgg tttaatgcta cgccaactca
    34741 tgcgccaggc gcgtcagcgt atcgccaaag gcgggagtgt gatccgcacc tcggtctcca
    34801 catttatgga gttcatcggt aataatccta acgccttccg gttattattg cgggaacgct
    34861 ccggcacctc cgctgcgttt cgtgccgccg ttgcgcgtga aattcagcac ttcattgcgg
    34921 aacttgcgga ctatctggaa ctcgaaaacc atatgccgcg tgcgtttact gaagcgcaag
    34981 ccgaagcaat ggtgacaatt gtcttcagtg cgggtgccga ggcgttggac gtcggcgtcg
    35041 aacaacgtcg gcaattagaa gagcgactgg tactgcaact gcgaatgatt tcgaaagggg
    35101 cttattactg gtatcgccgt gaacaagaga aaaccgcaat tattccggga aatgtgaagg
    35161 acgagtaatg aaacaagcaa atcaagatag aggtacgctg ctgctggcgt tagttgctgg
    35221 cttatcgatt aatggtactt tcgcagcgct gtttagctcc attgtgccat tttctgtatt
    35281 cccgattatt tccctggtgc tgacggttta ctgcctgcat caacgttatc ttaatcgcac
    35341 tatgccggta ggcttgccgg gtctggcagc tgcctgtttt attctcggcg tactgctgta
    35401 cagcacggta gttcgtgcgg aatatccgga tatcggctct aacttcttcc cggcagtact
    35461 ctccgtcatt atggtgttct ggattggcgc gaagatgcgt aaccgtaagc aggaagttgc
    35521 tgagtaatcg gtattatgcc ggatgctggc gcatccggca tgggttttac ttcgcggtca
    35581 gtaatacgcc gcactccata tggtgcgtgt aggggaactg atcaaacaga gccagacgtt
    35641 cgaccttgtg cgtctggctt aatgtttcca gattcttgca taacgtttcc gggttacagg
    35701 agatgtacaa aatacacgga tacgcctgca ccattttctc ggtttcactg tccagaccgc
    35761 tgcgcggtgg gtcgacaaaa atggtttcgc actgataact ctttaagtcg atcccttgca
    35821 ggcggttaaa ctcgcgcaca ccattcatcg cctgagtaaa ttcttctgcc gccatacgaa
    35881 taatttgtac gttatcaata tggttagctg cgatgttgta ttgcgcagca gcaaccgacg
    35941 gcttagcgat ttcggtggct aatacccgat caaaattgcg cgccagcgct aatgaaaagt
    36001 taccgttgcc gcagtacagc tccagtaaat cgccttttga gcctttggtt acgtccagcg
    36061 cccattccag catctgaata ttcatcgccg cgttcggctg ggtaaagctg ttttctacct
    36121 gacggtagat catctctttc cctgcgaccg gcagacgctc gtcgatgtaa tcttgatcca
    36181 gctcgatttt ggttttcgtt gcccgaccaa tcagatgcac attcagattc tgcgcgcgca
    36241 gtgcatcgcg cagggcctcc gcttcctgac gccactcatc atccagcttc ttatggtata
    36301 gcagggaaac caccgcctga ttactcagcg tggtgaggta atcaatctgg aacaacttgt
    36361 ggcgcagaac gggattatta cgcacacccg caatcatcgc cgtcatcaac tggttgataa
    36421 gttcactggc ggcggggaag ctatccacgc ggatacggct tttggtttgt tgatcgaaaa
    36481 tgatgtgata caggtcatcg ccatcgtgcc agatgcggaa ctccgcacgc atccggtaat
    36541 gactgaccgg cgagcgaaac acttccggaa ccaggtcaga aaacggtgcc atcatacttt
    36601 gcaaacgtac cactttttcg gctaactgcg cttcatactg ttctgttgga aggtgttcgg
    36661 gggtcatgat gtatcctgaa aaattaaagt acgcggcgat tgtagggatt gctcatcaga
    36721 tgtccagatc ttgatgaatt cctatttgtg agctacgtct ggacagtaac ttgttacaac
    36781 ctgtagcatc cacttgtcgg tcctgtgagt taatagggaa tccagtgcga atctggagct
    36841 gacgcgcagc ggtaaggaaa ggtgcgatga ttgcgttatg cggacactgc catccggtgg
    36901 gaagtcatca tctcttagta tcttagatac ccctccaagc ccgaagacct gccggccaac
    36961 gtcgcatctg gttctcatca tcgcgtaata ttgatgaaac ctgcggcatc cttcttctat
    37021 tgtggatgct ttacaatgat taaaaaagct tcgctgctga cggcgtgttc tgtcacagcc
    37081 ttttccgctt gggcacagga taccagcccg gatactctcg tcgttactgc taaccgtttt
    37141 gaacagccgc gcagcactgt gcttgcacca accaccgttg tgacccgtca ggatatcgac
    37201 cgctggcagt cgacctcggt caatgatgtg ctgcgccgtc ttccgggcgt cgatatcacc
    37261 caaaacggcg gttcaggtca gctctcatct atttttattc gcggtacaaa tgccagtcat
    37321 gtgttggtgt taattgatgg cgtacgcctg aatctggcgg gggtgagtgg ttctgccgac
    37381 cttagccagt tccctattgc gcttgtccag cgtgttgaat atatccgtgg gccgcgctcc
    37441 gctgtttatg gttccgatgc aataggcggg gtggtgaata tcatcacgac gcgcgatgaa
    37501 cccggaacgg aaatttcagc agggtgggga agcaatagtt atcagaacta tgatgtctct
    37561 acgcagcaac aactggggga taagacacgg ggaacgctgt tgggcgatta tgcccatact
    37621 catggttatg atgttgttgc ctatggtaat acaggaacgc aagcgcagac agataacgat
    37681 ggttttttaa gtaaaacgct ttatggcgcg ctggagcata actttactga tgcctggagc
    37741 ggctttgtgc gcggctatgg ctatgataac cgtaccaatt atgacgcgta ttattctccc
    37801 ggttcaccgt tgctcgatac ccgtaaactc tatagccaaa gttgggacgc cgggctgcgc
    37861 tataacggcg aactgattaa atcacaactc attaccagct atagccatag caaagattac
    37921 aactacgatc cccattatgg tcgttatgat tcgtcggcga cgctcgatga gatgaagcaa
    37981 tacaccgtcc agtgggcaaa caatgtcatc gttggtcacg gtagtattgg tgcgggtgtc
    38041 gactggcaga aacagactac gacgccgggt acaggttatg ttgaggatgg atatgatcaa
    38101 cgtaataccg gcatctatct gaccgggctg caacaagtcg gcgattttac ctttgaaggc
    38161 gcagcacgca gtgacgataa ctcacagttt ggtcgtcatg gaacctggca aaccagcgcc
    38221 ggttgggaat tcatcgaagg ttatcgcttc attgcttcct acgggacatc ttataaggca
    38281 ccaaatctgg ggcaactgta tggcttctac ggaaatccga atctggaccc ggagaaaagc
    38341 aaacagtggg aaggcgcgtt tgaaggctta accgctgggg tgaactggcg tatttccgga
    38401 tatcgtaacg atgtcagtga cttgatcgat tatgatgatc acaccctgaa atattacaac
    38461 gaagggaaag cgcggattaa gggcgtcgag gcgaccgcca attttgatac cggaccactg
    38521 acgcatactg tgagttatga ttatgtcgat gcgcgcaatg cgattaccga cacgccgttg
    38581 ttacgccgtg ctaaacagca ggtgaaatac cagctcgact ggcagttgta tgacttcgac
    38641 tggggtatta cttatcagta tttaggcact cgctatgata aggattactc atcttatcct
    38701 tatcaaaccg ttaaaatggg cggtgtgagc ttgtgggatc ttgcggttgc gtatccggtc
    38761 acctctcacc tgacagttcg tggtaaaata gccaacctgt tcgacaaaga ttatgagaca
    38821 gtctatggct accaaactgc aggacgggaa tacaccttgt ctggcagcta caccttctga
    38881 accacgtccc accgtgctgg tgtttgactc cggcgtcggt gggttgtcgg tctatgacga
    38941 gatccggcat ctcttaccgg atctccatta catttatgct ttcgataacg tcgctttccc
    39001 gtatggcgaa aaaagcgaag cgtttattgt tgagcgagtg gtggcaattg tcaccgcggt
    39061 gcaagaacgt tatccccttg cgctggctgt ggtcgcttgc aacactgcca gtaccgtttc
    39121 acttcctgca ttacgcgaaa agttcgactt cccggttgtt ggtgtcgtgc cggcgattaa
    39181 acctgctgca cgtctgacgg caaatggcat tgtcggatta ctggcaaccc gcggaacagt
    39241 taaacgttct tatactcatg agctgatcgc gcgtttcgct aatgaatgcc agatagaaat
    39301 gctgggctcg gcagagatgg ttgagttggc tgaagcgaag ctacatggcg aagatgtttc
    39361 tctggatgca ctaaaacgta tcctacgccc gtggttaaga atgaaagagc cgccagatac
    39421 cgttgtattg ggttgcaccc atttccctct actacaagaa gaactgttac aagtgctgcc
    39481 agagggaacc cggctggtgg attctggcgc agcgattgct cgccgaacgg cctggttgtt
    39541 agaacatgaa gccccggatg caaaatctgc cgatgcgaat attgcctttt gtatggcaat
    39601 gacgccagaa gctgaacaat tattgcccgt tttacagcgt tacggcttcg aaacgctcga
    39661 aaaactggca gttttaggct gatttggttg aatgttgcgc ggtcagaaaa ttattttaaa
    39721 tttcctcttg tcaggccgga ataactccct ataatgcgcc accactgaca cggaacaacg
    39781 gcaaacacgc cgccgggtca gcggggttct cctgagaatc tcaacagaga aaagcaaaaa
    39841 aatgcttgac tctgtagcgg gaaag
//
LOCUS       DAEEKQ010000019        39670 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.019, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000019 DAEEKQ010000000
VERSION     DAEEKQ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39670
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.019"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..268)
                     /locus_tag="KLK57_000991"
     CDS             complement(<1..268)
                     /locus_tag="KLK57_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891429.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTPAPV"
     gene            complement(428..640)
                     /gene="rpmE"
                     /locus_tag="KLK57_000992"
     CDS             complement(428..640)
                     /gene="rpmE"
                     /locus_tag="KLK57_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HBE0891430.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            843..3041
                     /gene="priA"
                     /locus_tag="KLK57_000993"
     CDS             843..3041
                     /gene="priA"
                     /locus_tag="KLK57_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HBE0891431.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMAVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNELKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQTLRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIINGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3197..4222
                     /gene="cytR"
                     /locus_tag="KLK57_000994"
     CDS             3197..4222
                     /gene="cytR"
                     /locus_tag="KLK57_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HBE0891432.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4314..5273
                     /gene="ftsN"
                     /locus_tag="KLK57_000995"
     CDS             4314..5273
                     /gene="ftsN"
                     /locus_tag="KLK57_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HBE0891433.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5366..5896
                     /gene="hslV"
                     /locus_tag="KLK57_000996"
     CDS             5366..5896
                     /gene="hslV"
                     /locus_tag="KLK57_000996"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HBE0891434.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            5906..7237
                     /gene="hslU"
                     /locus_tag="KLK57_000997"
     CDS             5906..7237
                     /gene="hslU"
                     /locus_tag="KLK57_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HBE0891435.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            7304..8230
                     /gene="menA"
                     /locus_tag="KLK57_000998"
     CDS             7304..8230
                     /gene="menA"
                     /locus_tag="KLK57_000998"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HBE0891436.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLVLITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8323..8808
                     /gene="rraA"
                     /locus_tag="KLK57_000999"
     CDS             8323..8808
                     /gene="rraA"
                     /locus_tag="KLK57_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HBE0891437.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(8893..9138)
                     /gene="zapB"
                     /locus_tag="KLK57_001000"
     CDS             complement(8893..9138)
                     /gene="zapB"
                     /locus_tag="KLK57_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HBE0891438.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            9564..10409
                     /gene="glpF"
                     /locus_tag="KLK57_001001"
     CDS             9564..10409
                     /gene="glpF"
                     /locus_tag="KLK57_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HBE0891439.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVAGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10432..11943
                     /gene="glpK"
                     /locus_tag="KLK57_001002"
     CDS             10432..11943
                     /gene="glpK"
                     /locus_tag="KLK57_001002"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HBE0891440.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDVM"
     gene            12073..13083
                     /gene="glpX"
                     /locus_tag="KLK57_001003"
     CDS             12073..13083
                     /gene="glpX"
                     /locus_tag="KLK57_001003"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HBE0891441.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13180..13926
                     /gene="fpr"
                     /locus_tag="KLK57_001004"
     CDS             13180..13926
                     /gene="fpr"
                     /locus_tag="KLK57_001004"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HBE0891442.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLEE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(13931..14359)
                     /gene="uspD"
                     /locus_tag="KLK57_001005"
     CDS             complement(13931..14359)
                     /gene="uspD"
                     /locus_tag="KLK57_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HBE0891443.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14386..14685)
                     /locus_tag="KLK57_001006"
     CDS             complement(14386..14685)
                     /locus_tag="KLK57_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HBE0891444.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(14897..15337)
                     /locus_tag="KLK57_001007"
     CDS             complement(14897..15337)
                     /locus_tag="KLK57_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBE0891445.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWFAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFVGTQGENKYGKDIQDVKYKADNKSSN"
     gene            15438..16037
                     /locus_tag="KLK57_001008"
     CDS             15438..16037
                     /locus_tag="KLK57_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HBE0891446.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNSEKGLTFAVEPIKLALSESLEGLNK"
     gene            16145..16912
                     /gene="tpiA"
                     /locus_tag="KLK57_001009"
     CDS             16145..16912
                     /gene="tpiA"
                     /locus_tag="KLK57_001009"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HBE0891447.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(16967..17722)
                     /gene="cdh"
                     /locus_tag="KLK57_001010"
     CDS             complement(16967..17722)
                     /gene="cdh"
                     /locus_tag="KLK57_001010"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HBE0891448.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(17829..18818)
                     /gene="sbp"
                     /locus_tag="KLK57_001011"
     CDS             complement(17829..18818)
                     /gene="sbp"
                     /locus_tag="KLK57_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HBE0891449.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19138..20100)
                     /gene="pfkA"
                     /locus_tag="KLK57_001012"
     CDS             complement(19138..20100)
                     /gene="pfkA"
                     /locus_tag="KLK57_001012"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HBE0891450.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20281..21183)
                     /gene="fieF"
                     /locus_tag="KLK57_001013"
     CDS             complement(20281..21183)
                     /gene="fieF"
                     /locus_tag="KLK57_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HBE0891451.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21331..21831)
                     /gene="cpxP"
                     /locus_tag="KLK57_001014"
     CDS             complement(21331..21831)
                     /gene="cpxP"
                     /locus_tag="KLK57_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HBE0891452.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            21981..22679
                     /gene="cpxR"
                     /locus_tag="KLK57_001015"
     CDS             21981..22679
                     /gene="cpxR"
                     /locus_tag="KLK57_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HBE0891453.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            22676..24049
                     /gene="cpxA"
                     /locus_tag="KLK57_001016"
     CDS             22676..24049
                     /gene="cpxA"
                     /locus_tag="KLK57_001016"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HBE0891454.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(24155..24829)
                     /gene="yiiM"
                     /locus_tag="KLK57_001017"
     CDS             complement(24155..24829)
                     /gene="yiiM"
                     /locus_tag="KLK57_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HBE0891455.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(24978..25961)
                     /gene="kdgT"
                     /locus_tag="KLK57_001018"
     CDS             complement(24978..25961)
                     /gene="kdgT"
                     /locus_tag="KLK57_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HBE0891456.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(26221..26841)
                     /gene="sodA"
                     /locus_tag="KLK57_001019"
     CDS             complement(26221..26841)
                     /gene="sodA"
                     /locus_tag="KLK57_001019"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HBE0891457.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            27126..28160
                     /gene="rhaT"
                     /locus_tag="KLK57_001020"
     CDS             27126..28160
                     /gene="rhaT"
                     /locus_tag="KLK57_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
                     /protein_id="HBE0891458.1"
                     /translation="MSNAITMGIFWHLIGAASAACFYAPFKKVKKWSWETMWSVGGIV
                     SWIILPWTISALLLPDFWAYYSSFSLSTLLPVFLFGAMWGIGNINYGLTMRYLGMSMG
                     IGIAIGITLIVGTLMTPIINGNFDVLINTEGGRMTLLGVLVALIGVGIVTRAGQLKER
                     KMGIKAEEFNLKKGLVLAVMCGIFSAGMSFAMNAAKPMHEAAAALGVDPLYVALPSYV
                     VIMGGGAIINLGFCFIRLAKVKDLSLKADFSLAKPLIIHNVLLSALGGLMWYLQFFFY
                     AWGHARIPAQYDYISWMLHMSFYVLCGGIVGLVLKEWNNTGRRPVTVLSLGCVVIIVA
                     ANIVGIGMAN"
     gene            complement(28157..29005)
                     /gene="rhaR"
                     /locus_tag="KLK57_001021"
                     /pseudo
     CDS             complement(28157..29005)
                     /gene="rhaR"
                     /locus_tag="KLK57_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
     gene            complement(29079..29915)
                     /gene="rhaS"
                     /locus_tag="KLK57_001022"
     CDS             complement(29079..29915)
                     /gene="rhaS"
                     /locus_tag="KLK57_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HBE0891459.1"
                     /translation="MTVLHSVDFFPSGNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRAHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            30203..31672
                     /gene="rhaB"
                     /locus_tag="KLK57_001023"
     CDS             30203..31672
                     /gene="rhaB"
                     /locus_tag="KLK57_001023"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HBE0891460.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACREMAQPLPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQLHSTRQTKELCA"
     gene            31669..32928
                     /gene="rhaA"
                     /locus_tag="KLK57_001024"
     CDS             31669..32928
                     /gene="rhaA"
                     /locus_tag="KLK57_001024"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HBE0891461.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQAIGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLDP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPWQAVWEMYC
                     QRHDTPAGSEWLESVRAYEKAILSQRG"
     gene            33170..33994
                     /gene="rhaD"
                     /locus_tag="KLK57_001025"
     CDS             33170..33994
                     /gene="rhaD"
                     /locus_tag="KLK57_001025"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HBE0891462.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YHGNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            34004..34318
                     /gene="rhaM"
                     /locus_tag="KLK57_001026"
     CDS             34004..34318
                     /gene="rhaM"
                     /locus_tag="KLK57_001026"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HBE0891463.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            34619..35065
                     /locus_tag="KLK57_001027"
     CDS             34619..35065
                     /locus_tag="KLK57_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HBE0891464.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQTLSS"
     gene            35076..36527
                     /locus_tag="KLK57_001028"
     CDS             35076..36527
                     /locus_tag="KLK57_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HBE0891465.1"
                     /translation="MESSLCIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            36517..37587
                     /locus_tag="KLK57_001029"
     CDS             36517..37587
                     /locus_tag="KLK57_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBE0891466.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVRHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKSCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            37587..39335
                     /locus_tag="KLK57_001030"
     CDS             37587..39335
                     /locus_tag="KLK57_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HBE0891467.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GVTRDNFQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDEWPQYPGNEIKQIV
                     EQSGMFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLHCRVIIARSLSELIAIREE
                     IEPLLIINNSHYLLDDAVNNYITVKNIITAADIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAQPVEVNNEVINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(39384..>39670)
                     /locus_tag="KLK57_001031"
     CDS             complement(39384..>39670)
                     /locus_tag="KLK57_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3829 domain-containing protein"
                     /protein_id="HBE0891468.1"
                     /translation="DVDAAMKKVSELETLVAQAKEADKGGMNFSFINSADQYQLETKK
                     YVRRVRDKVPYSDWDKEHLQDANTSWMVEDSFPRALREYNEMVDDYNSLR"
BASE COUNT         9631 a        10306 c        10317 g         9416 t
ORIGIN      
        1 ccaccggcgc gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca
       61 gcggggcggg cagggcgatg tcggtttcac cgggaaggac cttcgcaccg agcagcggat
      121 tgaccggatg gccggacgtc accccgccgg ggcacaccga acaggccacg ccggtggggg
      181 caccgatgcg caccccggct gaaccctgga caatgctacc gccatactgc gtcatgtcgc
      241 cctgacgcgc cgccggtttt ccgctcatcg taaaatcctt ttgaaataaa taagaaccag
      301 aagcctagcg ccagtcaaca acaggaaatg ataatgagtt aacaccccgg aatttttcag
      361 aatttataaa aggcaaatta caggcacaaa aaaagcgccg tgcggcgctt ttttcggaaa
      421 tccggtctta tttgctgccc gggatgttga aacgcttgtt gaagcggtca acacggccac
      481 cggtagcaac atcacgctgt ttgccagtga agaacgggtg gcacttgctg cacacgtcga
      541 ggttcaggtc atgaccaacg gtggagcgga ttttcattac gttaccgcaa gagcagctag
      601 cagtaatttc ttcgtatttc gggtgaatat cttttttcat gggaaaacct cggtttaagg
      661 ccgcgtcgct cttccagccc taacgccaga caccacgcga tgttaaaagt atagcttcaa
      721 tacgatcatt tcgtacgaag gcgcgaaatc atacagaaat taaccagcgt atgcaaactg
      781 atccgcactc ttctacggca atgtgtatac taacccaccg aatttcaagt caggatgatg
      841 ctatgcccgt tgcccacgtt gccttgcccg ttccgcttcc tcgtaccttt gactatctgc
      901 tgccagaagg catggcggtt aaagccgggt gtcgcgtgcg cgtgccgttt ggcaaacagc
      961 aggagcgcat cggggttgtg gtatcagtta gcgatgtcag tgagctgccg cttaacgagc
     1021 tgaaagcggt agttgaagtg ctggacgttg agccggtatt tactcactcc gtctggcgat
     1081 tactgctctg ggcggcagat tactatcatc atccgattgg cgatgtgctg tttcatgcct
     1141 tgccgatttt actgcgccag gggcggcctg cggcgaacgc gccgatgtgg tactggtttg
     1201 ccactgaaca aggccaggcg gtggatctga acagcctgaa acgctcaccc aagcaacaac
     1261 aggcgctggc ggcgttacgg caagggaaaa tctggcgcga ccaggttgcc acgctcgaat
     1321 ttaatgatgc cgcgttgcag acgctgcgca aaaaaggtct gtgtgattta gcaagtgaaa
     1381 caccagagtt tagcgactgg cgaacgaact atgccgtttc tggtgagcgg ttgcgattga
     1441 ataccgaaca ggccaccgcc gttggcgcaa ttcatagcgc ggcagatact ttttctgcct
     1501 ggctgctggc gggcgttacc ggttccggta aaacggaggt ttatctcagt gtactggaaa
     1561 acgtgctcgc tcagggcaaa caggcgctgg tgatggtgcc ggaaatcggc ctgacaccgc
     1621 aaactatcgc ccgttttcgt gaacggttta atgcgcccgt ggaagttctg cattccggtc
     1681 tgaacgacag cgagcgtctt tcggcgtggc tgaaagcgaa aaatggcgag gcggcgattg
     1741 tgatcggcac ccgctccgcg ctgtttacgc cgtttaaaaa tctcggcgtg attgtcattg
     1801 acgaagagca cgacagttcc tacaagcagc aggaaggctg gcgctatcat gcccgcgacc
     1861 tggcggtgta tcgtgcgcat agcgagcaaa tcccgattat tcttggctcc gcaacgcccg
     1921 cgctggaaac gttgtgcaac gtccagcaga aaaaataccg cctgctgcgc ctgacgcgcc
     1981 gggcggggaa tgcgcgtccg gcaattcaac acgtgctgga tttaaaaggt cagaaggtgc
     2041 aggcaggtct ggctccggcg ttaatcaccc gtatgcgcca gcatttacag gccaacaatc
     2101 aggttatcct ctttcttaac cgccgtggct ttgcgcctgc gctactgtgc cacgactgcg
     2161 gctggattgc cgaatgccca cgttgcgatc actactacac gctgcatcag gcgcaacagc
     2221 atttgcgctg tcaccactgt gacagtcagc gtccggtgcc gcgccagtgc ccttcctgcg
     2281 gttccacgca cctggtaccc gtggggctgg gcaccgaaca gcttgaacag acgcttgcgc
     2341 cgctgttccc tgatgtgccc atttctcgta tcgaccgcga taccaccagc cgcaaagggg
     2401 cgctggaaca gcaactggcg gaagtacatc gcggcggtgc gaggattttg atcggcacac
     2461 aaatgctggc gaaaggtcac cactttccgg atgtgacgct ggtggcgtta ctggatgtgg
     2521 acggcgcgct gttttctgcc gattttcgct cggcagagcg tttcgctcag ctttacaccc
     2581 aggtcgccgg tcgtgccggg cgtgcgggta aacagggcga agtggtgctg caaacgcacc
     2641 atccggaaca tcctctgttg caaacgttgc tctataaagg ctacgacgcc tttgccgaac
     2701 aggcgctggc tgagcgacgc atgatgcagc tgccgccgtg gaccagccat gtgattgtgc
     2761 gtgcggaaga tcataacaat cagcatgcgc cgctgttcct gcaacaactg cgtaatctga
     2821 tcctctccag cccgctggca gacgacaaat tgtgggttct cggtccggtt ccggcactgg
     2881 cacctaaacg tggcggtcgc tggcgctggc agatattgtt gcagcaccct tcccgcgtgc
     2941 gcttgcaaca catcattaac ggtacgctgg cactcatcaa tacaataccg gattcccgta
     3001 aggtgaaatg ggtgctggat gttgatccga ttgagggtta aaccgctcac gatgcgaggc
     3061 ggatcgaaaa attcaatatt catcacactt ttcatgaaaa ttctgtaacc gttttcacgc
     3121 gctatctgct aaaaatgttg ccgatgtgaa gtaaacatgg atgtagtacg cctgacgtgc
     3181 caggcgagga gtgagtgtga aagcgaagaa gcaggaaact gccgcgacca tgaaagacgt
     3241 tgccctcaag gcaaaagtct ctacagcgac tgtctcccga gcattaatga atcccgataa
     3301 agtctcccag gccacccgta atcgggttga aaaagcggcc cgggaagtgg gttatttacc
     3361 gcagcctatg gggcgcaacg tcaagcgtaa tgaatcccgc accattctgg tgattgtccc
     3421 ggatatctgc gatcccttct ttagcgaaat tattcgcggt atcgaagtta cggcggcaaa
     3481 tcacggatat ctggtgctga ttggcgactg tgcgcatcaa aatcagcagg aaaaaacctt
     3541 tatcgatttg atcatcacca agcaaattga tggcatgttg ctgctgggtt caaggctgcc
     3601 gtttgatgcc agcattgagg aacagcgtaa tctgccgccg atggtgatgg cgaacgaatt
     3661 cgcaccggaa ctggagctac ctacagttca tatcgacaat ctgaccgccg catttgatgc
     3721 agtaaattat ctatatgagc aagggcataa acggattggc tgtatagccg gtcccgaaga
     3781 gatgccgctg tgtcactacc gcctgcaagg ttacgttcag gcgctgcgtc gctgcggcat
     3841 tatggttgat ccgcaataca tcgcccgtgg cgacttcacc ttcgaagccg gaagcaaagc
     3901 gatgcagcag ctgcttgatc ttccacaacc gcctactgct gtcttctgcc atagcgatgt
     3961 gatggcgctc ggcgcacttt ctcaggcaaa acgccagggg ctgaaagtcc cggaagacct
     4021 ttccataatc ggttttgata acatcgacct gacgcaattt tgtgatccgc cgctgacaac
     4081 catcgcgcag ccgcgttacg aaatcggtcg ggaagctatg ctgttattgc ttgatcaaat
     4141 gcaggggcaa cacgttggca gtggctctcg tttaatggac tgcgaactta tcatccgggg
     4201 atcaacacgc gcgttacctt aaagtaaacc tttaagactg ccgtgactgg tcaaaggccc
     4261 gccgcttaag taacatggcg ggctgacgaa cgaataaata cagcgaaacg atagtggcac
     4321 aacgagatta tgtacgccgc agccaaccgg caccttcgcg gcgaaaaaag agcacctcac
     4381 ggaaaaagca acgaaatctg cctgcggttt ctcccgctat ggtcgctatt gctgccgccg
     4441 tgcttgtgac ctttatcggt ggtctgtact tcattacgca tcacaagaaa gaagagtccg
     4501 agacgctgca aagccagaaa gtgaccggta acggactacc accaaaacca gaagaacgct
     4561 ggcgctacat taaagagctg gaaagtcgcc agccgggtgt gcgtgcgccc acagaacctt
     4621 ctgccggtgg tgaagtgaaa acgccggagc aactgacacc agaacaacgt cagcttcttg
     4681 aacaaatgca ggctgatatg cgccagcagc caacgcagct ggttgaagtt ccgtggaatg
     4741 aacagacgcc ggaacagcgt cagcaaacgc tacaacgcca gcgtcaggcg cagcaattag
     4801 cggagcagca acgcctggct cagcagtcgc gtacgactga acaaagctgg cagcagcaga
     4861 cgcgtacgtc gcaagccgcg ccggtacagg cgcaaccgcg ccagtctaaa ccggcttcta
     4921 ctcagcagcc gtaccaggat ctgctgcaaa cccctgcgca cacgaccgcg cagtcgaagc
     4981 cgcagcaagc tgcgccggtt gctcgtgccg ctgacgcgcc aaaaccgacg gcggagaaaa
     5041 aagacgaacg ccgctggatg gtgcagtgcg gttcgttcag aggcgcggaa caggcagaga
     5101 cggtacgtgc tcagctggcg ttcgaaggat ttgactcgaa aatcaccacc aacaatggct
     5161 ggaatcgtgt ggtcattggc ccggtgaaag gcaaagagaa cgcagacagc accctcaatc
     5221 ggttgaagat ggcgggtcat acaaactgca ttcggctcgc cgccgggggt tgaaaccctc
     5281 aaaatccccc ccatctataa ttgcattatg ccccgtactt ttgtacgggg tttgtactct
     5341 gtattcgtaa ccaaggggtc agctcgtgac aactatagta agcgtacgcc gtaacggcca
     5401 tgtggtcatc gctggtgatg gtcaggccac gttgggcaat accgtaatga aaggcaacgt
     5461 gaaaaaggtc cgccgtctgt acaacgacaa agtcatcgcg ggctttgcgg gcggtactgc
     5521 ggatgctttt acgctgttcg aactgtttga acgtaaactg gaaatgcatc agggccatct
     5581 ggtcaaagcc gccgttgagc tggcaaaaga ctggcgtacc gatcgcatgc tgcgcaaact
     5641 tgaagcactg ctggcagtcg cggatgaaac tgcatcgctt atcatcaccg gtaacggtga
     5701 cgtggtgcag ccagaaaacg atcttattgc tatcggctcc ggcggccctt acgcccaggc
     5761 tgcggcgcgc gcgctgttag aaaacactga acttagcgcc cgtgaaattg ctgaaaaggc
     5821 gttggatatt gcaggcgaca tttgcatcta taccaaccat ttccacacca tcgaagaatt
     5881 aagctacaaa gcgtaaggat ctcccatgtc tgaaatgacc ccacgcgaaa tcgtcagcga
     5941 actggataag cacatcatcg gccaggacaa cgccaagcgt tctgtggcga ttgctctgcg
     6001 taaccgctgg cgtcgcatgc agctcaacga agagctgcgc catgaagtga ccccgaaaaa
     6061 tatcctgatg atcggcccga ccggtgtcgg taaaactgaa atcgcccgtc gtctggctaa
     6121 gctggcgaat gcgccgttca tcaaagttga agcgaccaaa ttcaccgaag tgggctatgt
     6181 cggtaaggaa gtggattcta ttattcgcga tctgaccgat gccgccgtga aaatggtacg
     6241 cgtccaggct atcgagaaaa accgttatcg cgctgaagaa ctggcagaag aacgtattct
     6301 cgacgtgctg atcccacctg ctaaaaacaa ctggggacag accgaacagc agcaggaacc
     6361 gtccgctgct cgtcaggcat tccgcaaaaa actgcgtgaa ggccagcttg atgacaaaga
     6421 aatcgagatc gatcttgccg cagcaccgat gggcgttgaa attatggctc ctccaggcat
     6481 ggaagagatg accagccagc tgcagtccat gttccagaac ctgggcggcc agaagcaaaa
     6541 agcgcgtaag ctgaaaatca aagacgccat gaagctgctg attgaagaag aagcggcgaa
     6601 actggtgaac ccggaagagc tgaagcaaga cgctatcgac gctgttgagc agcacgggat
     6661 cgtgtttatc gacgaaatcg acaaaatctg taagcgcggc gagtcttccg gtccggatgt
     6721 ttctcgtgaa ggcgttcagc gtgacctgct gccgctggta gaaggttgca ccgtttccac
     6781 caaacacggg atggtcaaaa ctgaccacat tctgtttatc gcttctggcg cgttccagat
     6841 tgcgaaaccg tctgacctga tcccggaact gcaaggtcgg ctgccaatcc gcgttgaact
     6901 gcaggcactg accaccagcg acttcgagcg tattctgacc gagccgaatg cctctatcac
     6961 cgtgcagtac aaagcactga tggcgactga aggcgtaaat atcgagttta ccgactccgg
     7021 tattaagcgc atcgcggaag cggcatggca ggtgaacgaa tctaccgaaa acatcggtgc
     7081 tcgtcgttta cacactgttc tggagcgttt aatggaagag atttcctacg acgccagcga
     7141 tttaagcggt caaaatatca ctattgacgc agattatgtg agcaaacatc tggatgcgtt
     7201 ggtggcagat gaagatctga gccgttttat cctataatcg cgttcaatca ttttcatcat
     7261 tgtttgatgg ggctgaaagg ccccattttt attggcgcgt attatgactg aacaacaaat
     7321 tagccgaact caggcgtggc tggaaagttt acgacctaaa accctccccc tcgcctttgc
     7381 tgcaattatc gtcgggacgg cgctggcatg gtggcaaggt cacttcgatc cgctggtcgc
     7441 cctgctggta ctaattaccg ccgggctatt acagatcctt tctaacctcg ccaatgatta
     7501 cggcgatgcg gtaaaaggca gcgataaacc tgaccgcatt gggccgctac gcggcatgca
     7561 aaaaggggtc attacccagc aagagatgaa acgggcgctc attattaccg tcgtgctcat
     7621 ctgtctctcc gggctggcac tggttgcagt ggcatgccat acgctggccg attttgtcgg
     7681 tttcctgatt cttggcgggt tgtcgatcat tgccgctatc acctacaccg tgggcaatcg
     7741 tccttatggt tatatcggtc tgggtgatat ttccgtactg gttttctttg gctggttgag
     7801 cgtcatgggg agctggtatt tacaggctca tacattgatt ccggcactga tccttccggc
     7861 gaccgcctgt ggcctgctgg caacggcagt actgaacatt aataacctgc gtgatatcaa
     7921 tagcgaccgc gaaaatggca aaaacacgct ggtggtgcgc ttaggtgaag tgaacgcgcg
     7981 tcgttatcat gcctgcctgc tgatgggctc tctggtatgt ctggcgctgt ttaatctctt
     8041 ttcgctgcat agcctgtggg gctggctgtt cctgctggcg gcaccattac tggtgaagca
     8101 agcccgttat gtgatgcggg aaatggaccc ggtggcgatg cgaccaatgc tggaacgtac
     8161 tgtcaaggga gcgttactga ctaacctgct gtttgtttta gggatattcc taagccagtg
     8221 ggcagcataa ctgacaaata tcaattaaca attgatgatt ttgccaacag cctacatagc
     8281 gcgatatact gaaaattctc gcagcaactg aatgttaagc ctatgaaata cgatacttcc
     8341 gagctttgtg acatctatca agaagatgtt aacgtcgtgg aaccgctgtt ctccaacttt
     8401 ggcggacggg cgtcgtttgg cggacaaata atcacggtaa aatgtttcga ggacaacggg
     8461 ttgctgtacg atctgctcga acagaatggc cgtggtcgtg ttcttgtcgt cgatggcggt
     8521 ggttctgttc gtcgcgcact ggtcgatgct gaactggcgc gtctggcagt acaaaatgaa
     8581 tgggaaggtc tggttattta cggcgcggtg cgtcaggtag atgacctaga agagttggat
     8641 atcggcatcc aggcgatggc ggcaattccg gttggtgccg ctggcgaagg cattggcgaa
     8701 agcgatgtcc gcgtcaattt tggcggtgta accttcttct ccggcgacca tctttatgcc
     8761 gacaataccg ggattattct ttcagaagat ccgctggata ttgaatgata agaaaggcac
     8821 cgcaaggtgc cttttttctg cgttacctgt tggcctacac agtaaagaaa ttacgcgaaa
     8881 gatgaagcgt aatcagacct cttccatgcg acccagcagg gcctgcagac gttcctgcca
     8941 gccgttctgc tgttctttca gatggttgtt ctcacgctcc agctcttcgc gctgatgctg
     9001 ggcattttga acttcctgcg acagtgagtt gtttttttct ttcagctctt cgatttccat
     9061 ctgcaacaga gtgatggtat caatcgcctg ctgtactttt gcttccagtt tctcaaacac
     9121 ttctaatgac attgtcatac ctctcctgaa ttgcaaggcg ttgatggata aaaaatcctc
     9181 gtcccgatta ccggtgacgc cttaataaat acgagcgcac tttagttagc tccgattgta
     9241 tgaagccgcg ccatcgctgt ccagcggcac gccttgcaga ttacggtttg ccacactttt
     9301 catccttctc ctggtgacat aatccacatc aatcgaaaat gttaataaat ttgttgcgcg
     9361 aatgatctaa caaacatgca tcatgtacaa tcagatggaa taaattgcgc gataacgctc
     9421 attttatgac gaagcacaca cattttaagt tcgatatttc tcgtttttgc tcgttaacga
     9481 taagtttaca gcatgcctac aagcatcgtg gaggtccgtg actttcacgc atacaacaaa
     9541 cattaactct tcaggatccg attatgagtc aaacatcaac cttgaaaggc cagtgcattg
     9601 ctgaattcct cggtaccggg ttgttgattt ttttcggtgt gggttgcgtt gcagcactaa
     9661 aagtcgctgg tgcgtctttt ggtcagtggg aaatcagtgt catttgggga ctgggggtgg
     9721 caatggccat ctacctgacc gcaggggttt ccggcgcgca tcttaatccc gctgttacca
     9781 ttgcattgtg gctgtttgcc tgtttcgaca agcgcaaagt tattcctttt atcgtttcac
     9841 aagttgccgg cgctttctgt gctgcggctt tagtttacgg gctttactac aatttatttt
     9901 tcgacttcga gcagactcat cacattgttc gcggcagtgt tgaaagtgtt gatctggcag
     9961 gcactttctc tacttaccct aatcctcata tcaattttgt gcaggctttc gcagttgaga
    10021 tggtgattac cgctattctg atggggctga tcctggcgtt aacggacgat ggcaacggtg
    10081 taccacgcgg ccctttggct cccttgctga ttggtctact gattgcggtc attggcgcat
    10141 ctatgggccc attgacgggt tttgccatga acccagcgcg tgacttcggt ccgaaagtct
    10201 ttgcctggct ggcgggctgg ggcaatgtcg cctttaccgg cggcagagac attccttact
    10261 tcctggtgcc gctttttggc cctatcgttg gcgcgattgt aggtgcattt gcctaccgca
    10321 aactgattgg tcgccatttg ccttgcgata tctgtgttgt ggaagaaaag gagaccacaa
    10381 ctccttcaga acaaaaagct tcgctgtaat atgactacgg gacaattaaa catgactgaa
    10441 aaaaaatata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    10501 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    10561 ggctgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    10621 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    10681 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    10741 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    10801 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    10861 tggatcctcg accatgtgga aggctctcgc gagcgtgcgc gtcgtggtga attgctgttt
    10921 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    10981 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    11041 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    11101 gtgtacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    11161 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    11221 aacacctatg gcactggctg ctttatgctg atgaacaccg gcgagaaagc ggtgaaatca
    11281 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgctg
    11341 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    11401 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    11461 tatgtggttc cggcctttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    11521 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    11581 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    11641 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    11701 tccgatattc tcggcacccg cgttgagcgc ccggaagttc gcgaagtgac tgcattgggc
    11761 gcggcctatc ttgctggtct ggcggtcggt ttctggcaga acctcgacga gctgcaagag
    11821 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    11881 tacgcaggct ggaaaaaagc ggttaaacgc gcgatggcgt gggaagaaca cgacgtaatg
    11941 taaatgccgg atgaagcgtt tatgccgcat ccggtagtcc cgaaacgtgc gggggcaacc
    12001 ccgcacacat caataatccc tcccttcccc tgtgctacac ttcgcgccat tccttactgc
    12061 ttagagtttg ctatgagacg agaacttgcc atcgaatttt cccgcgtcac cgaatctgcg
    12121 gcgctggctg gctacaaatg gttaggacgc ggcgataaaa acaccgcgga cggcgcggcg
    12181 gtaaacgcca tgcgtattat gctcaaccag gtcaatattg acggcaccat cgtcattggt
    12241 gaaggtgaaa tcgacgaagc accgatgctc tacattggtg aaaaagtcgg tactggtcgc
    12301 ggcgacgcgg tagatattgc tgttgatccg attgaaggca cgcgcatgac ggcgatgggc
    12361 caggctaacg cgctggcggt gctggcagta ggcgataaag gctgcttcct caatgcgccg
    12421 gatatgtata tggagaagct gatcgtcgga ccgggagcca aaggcaccat tgatttgaac
    12481 ctgccgctgg cggataacct gcgcaatgtt gcggcggcgc tcggcaaacc gttgagcgaa
    12541 ctgacggtaa caattctggc taaaccacgc cacgatgccg ttatcgctga aatgcaacaa
    12601 ctcggcgtac gcgtatttgc tattccggat ggcgacgttg cggcctcaat tctcacctgt
    12661 atgccagaca gcgaagttga cgtgctgtac ggtattggtg gcgcgccgga aggcgtagtt
    12721 tctgcggcgg tgatccgcgc attagatggc gacatgaacg gtcgtctgct ggcgcgtcat
    12781 gacgtcaaag gcgacaacga agagaatcgt cgcattggcg agcaggagct ggcacgctgc
    12841 aaagcgatgg gcatcgaagc cggtaaagta ttgcgcctgg gcgatatggc gcgcagcgat
    12901 aacgtcatct tctctgccac cggtattacc aaaggcgatc tgctggaagg cattagccgc
    12961 aaaggcaata tcgcgactac cgaaacgctg ctgatccgcg gcaagtcacg caccattcgc
    13021 cgcattcagt ctatccacta tctggatcgc aaggacccgg aaatgcaggt gcacatcctc
    13081 tgattgattt gatcgattga gccttccagt ccttcgggac tggaattttt ttgttcggag
    13141 aacgaagata aggcaagtca atcaaaacag gagaaaaaca tggctgattg ggtaacaggc
    13201 aaagtcacta aagtgcagaa ctggaccgac gccctgttta gtctcaccgt tcacgccccc
    13261 gtgcttccgt ttaccgccgg gcaattcacc aagcttggcc ttgaaatcga cggcgaacgc
    13321 gtccagcgcg cctactccta tgttaactcg cccgataatc ccgatctgga gttttacctg
    13381 gtcaccgtcc ccgatggcaa attaagccca cgactggcgg cactgaaacc aggcgatgaa
    13441 gtgcaggtgg ttagcgaagc agctggcttc tttgttctgg aggaagtacc agattgcgaa
    13501 acgctatgga tgctggcaac cggtacagcg attggccctt atttatcgat tctgcaacta
    13561 ggcaaagatt tagatcgctt caaaaatctg gtcctggttc acgccgcacg ttatgccgcc
    13621 gacttaagct atttgccact gatgcaagaa ctggaaaaac gctacgaagg caaactgcgc
    13681 attcagacgg tggtcagtcg ggaaacggca gcggggtcgc tcaccggacg gataccggca
    13741 ttaattgaaa gtggggaact ggaaagcgcg attggcctgc cgatgaataa agaaaccagc
    13801 catgtgatgc tgtgcggcaa tccacagatg gtgcgcgata cacaacagtt gctgaaagag
    13861 acccggcaga tgacgaaaca tttacgtcgc cgaccgggcc atatgacagc ggagcattac
    13921 tggtaagcgg ttacttatcg ataaacggca cgatgagcaa atccgcactc atcttattga
    13981 tcatcccgcg atatgccggc atcaaacggt tgataaatga gtggtgatga ccacagacaa
    14041 ggaggtcgca ctgctctttt tgcataattt ccagcagtgt ttccggcatt tctccgcgtt
    14101 caatacgcag ttttgtcttc ggccattgaa tatttttcgt cagtttatac agcttgttat
    14161 ccgacttatt cttcaacaat tgaagaatat cttctgttgc agggaagtag atacccggat
    14221 acaactcgct taagccatca tcaatatgaa ttaacgtcag gtgagcgtca ttatgtctgg
    14281 cgagctccag ggctttattc accagtaagg catcttcttc attcccggaa attgccacgc
    14341 caatgtgttt ataagccatg tttaactcct tctaaagcaa ctccatcaag ctaacgaacg
    14401 cagtgataac tcaaaaataa tcatctcggc ctggcatgag aaagtgaagg ccgcatcaag
    14461 ctcaacttgc ccctccagat ccttaatctt ataggccacg ctggcccctt catctgcaga
    14521 ggatgcagcg gcggcaagct ctttcagttt ggtcataaaa gactctgctt cttcgcgggt
    14581 ggcaaaaaag cgcgaaaact tactggtaca gttgtcgtta tcaatcaccg taccgatatc
    14641 aatcgcacat cctttagtac tgcatttatc tacgacatct ttcatagggc acctctgtat
    14701 attcgcctct ctgtcgatta cgacttaaga ataatgcgcc gttaagggaa gaacaaacaa
    14761 gtaatgatct tatggattac catttttttt gatattgctt aaggtgaaga gttcagaagg
    14821 attttccggg tattgtcatt tgcatcgtgg aaataacaat cccggaaaaa aatagcagta
    14881 agagatatat taaaggttaa ttacttgatt tattgtcggc tttatacttc acatcctgaa
    14941 tatctttacc atatttattt tcgccttgtg tgccaacaaa cgcaccaaga tcgataagca
    15001 tcatcaccag aatcaacgtc gggacaaaac gccccaccgc ccattgccag acacccggta
    15061 aaatcgccca gttacccgcc agcagcatcc acgccacaat catcagaaat gcccatgcgc
    15121 cggaacgccc gcgatcatgc aagcgcttaa cagttactgc cgctgttggc caaagcaagc
    15181 acacaaggca aaacgccgcg gtctgaatat cgagtaaatt tttacccgcc agtgagaaaa
    15241 gcaccagcat gcctgcgaac cacaggccta tccaaatcca gaaatcacgg cgtccaatac
    15301 gccctttaaa tgagaataac cattgctgta tggtcatgta agttccttga tggttgtctt
    15361 ttccaggatt ctaccctttt gacaagggcg acagatatcg ttttaatcgg agccagtcat
    15421 aacaaaaggt actgtcaatg aagccagggt gtacgctgtt ttttctctta tgttctgcat
    15481 taaccgttac aacaacggcg catgcacaaa caccagatac ggcaacgacc gcgccttatc
    15541 tgctggctgg agcccctact ttcgatctct ccatcagcca gtttcgagaa gactttaaca
    15601 gccagaatcc tagcctgcca ctgaacgaat ttcgtgccat cgacagcagt cccgacaaag
    15661 ccaatctcac tcgtgctgca agtaaaatta acgagaactt gtatgcttct acagcgctgg
    15721 agcgcggtac cttaaaaatc aaaagcattc aaatgacctg gctacccatc caggggccag
    15781 agcaaaaagc agcgaaagcg aaagctcagg aatacatggc agcagtgatc cgcacactca
    15841 ccccattaat gaccaaaaca caaagccaga aaaaactgca gtcgctacta acggcgggga
    15901 aaaacaaacg ttattacacc gagacagaag gtgcactgcg ttatgttgtc gcggacaaca
    15961 gcgaaaaggg gctgaccttc gctgttgaac cgattaagct ggcgctatct gaatcgcttg
    16021 aaggtttgaa taaatgacaa aaagcaaagc ctttgtgccg atgaatctct atactgtttc
    16081 acagacctgc tgccctgcgg ggcggccatc ttcctttatt cgcttataag cgtggagaat
    16141 taaaatgcga catcctttag tgatgggtaa ctggaaactg aacggcagcc gccacatggt
    16201 tcacgagctg gtttctaacc tgcgtaaaga gctggcaggt gttgctggct gtgcggttgc
    16261 aatcgcacca ccggaaatgt acatcgatat ggcgaagcgc gaagctgaag gcagccacat
    16321 catgctgggt gcgcaaaacg tggacctgaa cctgtccggc gcattcaccg gtgaaacttc
    16381 tgccgctatg ctgaaagaca tcggcgctca gtacatcatc atcggccact ctgaacgtcg
    16441 tacttaccac aaagagtctg acgaactgat cgcgaaaaaa ttcgcggtgc tgaaagagca
    16501 gggcctgact ccggttctgt gcatcggtga aaccgaagct gaaaacgaag cgggcaaaac
    16561 tgaagaagtt tgcgcacgtc agatcgacgc ggtactgaaa actcagggtg ctgcggcatt
    16621 cgaaggtgcg gttatcgctt acgaacctgt atgggcaatc ggtactggca aatctgcaac
    16681 tccggctcag gcacaggctg ttcacaaatt catccgtgac cacatcgcta aagttgacgc
    16741 taacatcgct gaacaagtga tcattcagta cggcggctct gtaaacgcgt ctaacgctgc
    16801 agaactgttt gctcagccag atatcgacgg cgcgctggtt ggcggtgctt ctctgaaagc
    16861 tgacgctttc gcagtgatcg ttaaagctgc agaagcggct aaacaggctt aagtctgaca
    16921 ggtgccggat ttcatatccg gcacttactt tccttaactc ttcgccttaa cgcaaaatct
    16981 cacactgatg atcctgaatt tcctcggctg aagcacggtt aagcgtcagt agatttcgtt
    17041 gtgtcgccag caatacaaat gagttatcac tctgccgtac catcgccagc ccgtagcttc
    17101 ccatatgttc ccgcgcctca ggtacttctt ctgccagcat cataaatggg ctgcgttgta
    17161 ccagttcgct ttccgttacc cgacgcgcca ggtattcatg cccacgcaaa ccacctggca
    17221 gtggcaacca gcggctgctg atgttcgcca gattgttatc cagttgttcg cgcacatcag
    17281 gacgaataca agagatatga atatgaaaat ggttttgcgt acgcccggtg cgggagttga
    17341 tcgccaaaga aaccgcgcga tcgggaaccg gctggccgta ttttttgctc ataaaatcac
    17401 gcgcctgcca ggccaaccaa aagaagttcg gcgttgaagg atcggtcaac aaaggacttt
    17461 cagtaccgtt aatacgatac gttggcatca acagatattg cagtggacca ttaagatctt
    17521 ttaaaaccac gtatccggca ttgggtttga cttccgcaca tggcgaagga ttttgatttt
    17581 gctgctgatt gggcaaacat tcctcaagga caatcttacg taatgtatcc gactcttcac
    17641 cggttaattt ccagtaacca ataccggcag ccacaacggc gataactatc atcaccaaaa
    17701 aaagaagacc cgcttttttc atcttttttt ccctgtacct caaagagatg caagggtaac
    17761 gcaaaatcgt gacaaataaa aaacccggtc gcgagaaacc accgggtttg ataattatcc
    17821 tggggaaatc agcgtttgct gatctgatcg aacgtaccgc cgttagcaaa atgctctttt
    17881 tgcgctttcg tccagccgcc gaactcttca tcaatggtga ataacttcag ctttggaaac
    17941 gcattttcgt actttttcgc tacttcagcg tcgcgcggac ggtagtagtt tttcgcggca
    18001 atttcctgac cttctggcga gtagagatat ttcaggtagg cttccgccac ctctttggtg
    18061 ccttttttct cgaccacttt atcgaccacc gacacggttg gctctgcgag gatagactca
    18121 ctcggcgtga cgatttcgaa tttatctttc cccagttcat tcgctgccag cagagcttcg
    18181 ttttcccagg cgatcagtac atcgccaatc ccgcgctcga caaaggtgtt agtggagccg
    18241 cgcgcgccag aatccagaac ttcgacgttt ttatacagtg cccgaacaaa atcctgtgct
    18301 tttgcctgat cgttgttgtt gtgatgcagc gcgtagcccc aggcagctag gtagttccag
    18361 cgtgcgccac cagagctttt cggattaggc gtgatcaccg aaacacccgg tttaatcaga
    18421 tcgttccagt catggatctg cttcggattg cccttacgca ccaggaaaac aatggtggaa
    18481 gtgtacggtg cggagttatc cggcagacgt ttgatccact ctttatcaat ccgcccacgt
    18541 tccgcaatcg cgtccacgtc ataggccaga gccagcgtga caacatcagc ttcaataccg
    18601 ttgattaccg acgtcgcttg tttacctgaa ccaccgtgtg actgacgaat caccacgtta
    18661 tcaccagttt gctgtttcca gtgggcgctg aatgccttgt tgtactgttc gtacaattcg
    18721 cgcgttggat catatgaaac gttaagaagc tgaatatcct ttgccataac gctggttgcc
    18781 gccagcaaaa atgttaaccc tacgccccac ttgttcatcg cccgactctc ttatgttgtg
    18841 ttgtgatgag caaagcgtgc cagaaggtta accaaacatt aaagaataaa aaaagattgg
    18901 ctataacttg cgggtatatg ttgagggatt aaaaaggcgg aagaaatccg cctcatattg
    18961 ctgacaaagt gcgctttgtc catgccggat gcggcgcgaa cgccttatcc ggcctacaaa
    19021 agtttgcaaa ttcaataaat tgcagaattc atgtaggcct gataagcgaa gcgcatcagg
    19081 catttttgct tctgtcatcg gtttcaggct aaaggaatct gcctttttcc gaaatcatta
    19141 atacagtttt ttcgcgcagt ccagccagtc acctttgaac ggacgcttca tgttttcgat
    19201 agcgtcgatg atgtcgtggt gaaccagctg ttcgttctgg atacctacac aacgaccgcc
    19261 gtaacctgcc agcagcagat cgatagcgta agcgcccata cgggaagcca gaatacggtc
    19321 gtaaggcacc ggagaaccac cgcgctggat gtggcccagc acagttgcgc gggtttcacg
    19381 accggtttct ttctcgatga aatgcgccag ttcgtcaaca tcacacatat gttcggtaat
    19441 cgccacgatc gcgtgttttt tacctttcgc gatacccgct ttgatttcgt ttaccaggtc
    19501 ttcacggctg aattcaactt ccggaaccac aacgaattca cagccaccgg caatggccgc
    19561 agccaacgtc agatcgccac aataacggcc catcacttcc accacggaaa tacgctggtg
    19621 agaagaagag gtgtcacgca gacggtcgat cgcttctaca acggtgctca gcgcagtgaa
    19681 gaaaccgata gtgtagtcag tgcctttgat gtcgttgtcg atagtgcccg gcagaccgat
    19741 gcacgggaag cccatttcgg tcagacgcat tgcacccatg taggaaccgt caccgccgat
    19801 aaccaccagc gcgtcgatac cacgtttttt caggttttcg atagccacgg cgcggatgtt
    19861 ctcgtcgcgg aattccggga agcgcgcaga accgaggaac gtaccgccac ggttgatcat
    19921 gtcagaaacg ctgtaacggt ctagctgtac catacggtct tcatacagac ccagatagcc
    19981 gtcataaatg cccattactt ccagaccttc tgtcagcgca gaacgaacaa ccccgcgaat
    20041 tgcggcgttc atgcctggcg catcaccgcc gcttgtcaac acaccgattt tcttaatcat
    20101 gactacctct gaactttgga atgcaaaata aaatctgttg ccggaagtct tcttgcacat
    20161 cgaagtgatc caacgaatgt gcaaatagta taacaatcac ttcctgctga attgattcag
    20221 gtcaggccaa atggcggtat tttatacaca aaatgcgggt ctggctctct tttatactga
    20281 ttatgaaagc atagaccgtt taccctccct gggtacgacg gaacaggggt cctgatggat
    20341 aattacatcc gatcccggaa aacgccgtaa aatagcctgc tctacctgat ccgccaccat
    20401 atgtgcctga accaaaggca gagagtcttc catttccaaa tgaatctgaa taaagcgggt
    20461 cggccctgac tgccgcgtgc gaagatcgtg agcgccgcta acacccggcc aggaagtcac
    20521 gatatcaata atttcttgcc gttcctcatc aggcaatgcg cgatccagta atgactgtac
    20581 cgcctcatat cccatgcgta acgcgctata taaaatatag atgccgattc ccaatgcaaa
    20641 cagagcatcg gcgcgatgcc agccgtacca ggacaacccc agcgccagca gaattgcgcc
    20701 gttcatcata acatcagact ggtaatgtag catatcagcc cgcaccgcct ggctttgcgt
    20761 ccggcgcacc acccaccgct gaaacgagac aaggataatc gtacaaatta gcgccacaat
    20821 tgtcacgata accccgacgc ctggatctgt catcggtgtt ggagatacca gatgttgaat
    20881 acccgtcaaa aacaggaata gtgccgaacc ggagataaac atactttgcg ccagcgccgc
    20941 gagggactca gctttaccgt gaccaaacga gtgattatcg tcggcaggtt gcagggaata
    21001 tcgcaccacc agtaaattcg tcaacgacgc gccgatatcc accagcgaat ccaccagcgc
    21061 ggcgagaata ctcaccgacc cggtatacca ccatgcaaaa attttaatca gcaatagcag
    21121 cgaagccatc gccgtcgcag caatcgccgc ccgactgacc agccgtccat aagattgatt
    21181 cataaatact cccactatca actgacgcta gtataacgga agcaaatcat ctgcaatgca
    21241 ttaagcagca ggcaaattga ggataaaaaa aaccccacat catgtggggg aagacaggga
    21301 tggtgtctat ggcaaggaaa acagggttta ctactgggaa cgtgagttgc tactactcaa
    21361 tagcttcaac gatgaacttt tttgccattg cgtcacgtca cgcaactgct ccattcgttg
    21421 ttgatgtttc tcgtttaaaa ccgcttgctg ctccggcgtt aacaggcgat acatttggtt
    21481 gcggactttt gccatctcaa cctgacgagc aatttgctca ttcgccattt tttctgcctg
    21541 tgcgcgcaca gcgttttcat caaaattttc tgcggtgaca aggcgatgca ttgtctccag
    21601 ttcgctaaca ttaacaggag gctgttcgtg ccgggcctgt tgcataagat ctcgcatctg
    21661 ctgacgctga tgttcggtta aacttatgcc gtcgaacata tggctctgcg tactgcgctg
    21721 cgtaagttct tcacccggat gccagttatc gcctgaaccg acttcagcag cgtggcttaa
    21781 tgaactgact gccagcgttg aggccatgac ggcagcggta actatgcgca tcatttgctc
    21841 ccaaaatctt tctgtcgcga ttcaacgata gagagtttac gattcaggct gcaaacatgc
    21901 gtcagggggt gtaaaacaac gtaaagtcat ggattagcga cgtctgatga cgtaatttct
    21961 gcctcggagg tatttaaaca atgaataaaa tcctgttagt tgatgatgac cgagagctga
    22021 cttccctatt aaaggagctg ctcgagatgg aaggcttcaa cgtgattgtt gcccacgatg
    22081 gggaacaggc gcttgatctt ctggacgaca gcattgattt acttttgctt gacgtaatga
    22141 tgccgaagaa aaatggtatc gacacattaa aagcacttcg ccagacacac cagacgcctg
    22201 tcattatgtt gacggcgcgc ggcagtgaac ttgatcgcgt tctcggcctt gagctgggcg
    22261 cagatgacta tctcccgaaa ccgtttaatg atcgtgagct ggtggcacgt attcgcgcga
    22321 tcctgcgccg ttcgcactgg agcgagcaac agcaaaacaa cgacaacggt tcaccgacac
    22381 tggaagttga tgccttagtg ctgaatccag gccgtcagga agccagcttc gacgggcaaa
    22441 cgctggagtt aaccggtact gagtttaccc tgctctattt gctggcacag catctgggtc
    22501 aggtggtttc ccgtgaacat ttaagccagg aagtgttggg caaacgcctg acgcctttcg
    22561 accgcgctat tgatatgcac atttccaacc tgcgtcgtaa actgccggat cgtaaagatg
    22621 gtcacccgtg gtttaaaacc ttgcgtggtc gcggctatct gatggtttct gcttcatgat
    22681 aggcagctta accgcgcgca tcttcgccat cttctggctg acgctggcgc tggtgttgat
    22741 gttggttttg atgttaccca agctcgattc acgccagatg accgagcttc tggatagcga
    22801 acagcgtcag gggctgatga ttgagcagca tgtcgaagcg gagctggcga acgatccgcc
    22861 caacgattta atgtggtggc ggcgtctgtt ccgggcgatt gataagtggg caccgccagg
    22921 acagcgtttg ttattggtga ccaccgaagg ccgcgtgatc ggcgctgaac gcagcgaaat
    22981 gcagatcatt cgtaacttta ttggtcaggc cgataacgcc gatcatccgc agaagaaaaa
    23041 gtatggccgc gtggaactgg tcggtccgtt ctccgtgcgt gatggcgaag ataattacca
    23101 actttatctg attcgtccgg ccagcagttc tcaatccgat ttcattaact tactgtttga
    23161 ccgcccgcta ttactgctga ttgtcaccat gttggtcagt acgccgctgc tgttgtggtt
    23221 ggcctggagt ctggcaaaac cggcgcgtaa gctgaaaaac gctgccgatg aagttgccca
    23281 gggaaactta cgccagcacc cggaactgga agcggggcca caggaattcc ttgccgcagg
    23341 tgccagtttt aaccagatgg tcaccgcgct ggagcgcatg atgacctctc agcagcgtct
    23401 gctttctgat atctctcacg agctgcgcac cccgctgacg cgtctgcaac tgggtacggc
    23461 gttactgcgc cgtcgtagcg gtgaaagcaa ggaactcgag cgtattgaaa ccgaagcgca
    23521 acgtctggac agcatgatca acgatctgtt ggtgatgtca cgtaatcagc aaaaaaacgc
    23581 gctggttagc gaaaccatca aagccaacca gttgtggagt gaagtgctgg ataacgcggc
    23641 gttcgaagcc gagcaaatgg gcaagtcgtt gacagttaac ttcccgcctg ggccgtggcc
    23701 gctgtacggc aatccgaacg ccctggaaag tgcgctggaa aacattgttc gtaatgctct
    23761 gcgttattcc catacgaaga ttgaagtggg ctttgcggta gataaagacg gtatcaccat
    23821 tacggtggac gacgatggtc ctggcgttag cccggaagat cgcgaacaga ttttccgtcc
    23881 gttctatcgg accgatgaag cacgcgatcg tgaatctggc ggtacaggat tggggctggc
    23941 gattgttgaa accgccattc agcagcatcg tggctgggtg aaggcagaag acagcccgct
    24001 gggcggttta cggctggtga tttggttgcc gctgtataag cggagttaaa ctccgcattt
    24061 gtaggcagga taaggcgttt acgccgcatc cggcatttga gcaggatgcc tgatgcgacg
    24121 ctgatagcgt cttatcaggc ctacactccg ggggttattt tccccacaac cggcgggaaa
    24181 aatcttcgat cttgccgctc agtcggcgct tttgcatcgt ccgcgtccag cttttcgata
    24241 acccggcggc ggagagtaaa cggtgatact gatcgtcatc aaacggcata tgccatgcga
    24301 tggcggcagc ctcctgcacg gtcacatcgc tgacacggga aaccaactcc agcggcgcgt
    24361 ccgcagacac tttccctggc gctatcacgc tatacaacca ccccacctta cctgtgtttt
    24421 gcatcaactg cgctatatcg ctgatatcaa aatggtaatt gagtttatag caaggcgagc
    24481 gcggctggct gacctgaatt aatgcctctc cccagcggaa aatatcgccc atatgaacat
    24541 tactttccgt gagaccgtcg gttgagagat tttcgccaaa cgcaggcgca acaaacaact
    24601 ccgcctgttc cggaaattcc cgcacccagt agagatagtg ctcacgagga taatgacaca
    24661 gcgctctgtc tggcccgccg tgaacctttt tctccgcctg ctcgtcacct tccagcccca
    24721 gctccgtcag catcaactca ccatcaacct ggattttggc aattgcgctg ggtttgcctt
    24781 cgggataagc ctgaatcttg cctgtgtata catcaaccgg atatcgcatc tgttccctct
    24841 ccacgagaaa tttccccatt agaccatatt tattttatgt caggcgtgct gttttcgcgg
    24901 taagtatcca ggcaggaact caatagcaaa ccggacgaac gttagttcat ccggctgtgg
    24961 ggattaagga agcaggttta tttcaccgta cctaaaattt cgattttcgc tgctctggct
    25021 ttgactttac gtgaccagat agaggtaaga attggcacca gaatcgaagt cacaatgacc
    25081 gccgtcgcta ccagcgaagt tgctgccgga gccatcggtt taaacgcagg caccatttct
    25141 gcaatcagca caggtgtcgc taccgcggcc cctgcggaac tggaagcggc aattccggca
    25201 gtgccatcgc caccgccaat caatttatcg gcgataatca acggaatacc ggtcacgata
    25261 attactgcca cacccaacag gatccccagc aaaccagtct gggcaatcac agtcaaatca
    25321 atggtattgc ccagcgcgaa ggcaaagaat ggaatcagcg tttgcactgc tttgctgaaa
    25381 aattctcgta attcagggtc aagattcccc agggcaaagc ccaccaggaa cggtaatact
    25441 gcgccgacga aaacatgcgg ttcaaacgag gcaatcccgg cagtgcccag aataatcatc
    25501 gtcatgagcg gcccggactc caacgacatc aacacaaatg ccccagcttc ttctttcgta
    25561 ccgtactgct gcatgatgga agcgtaaagt ccgccgttgg tcatatccat cgccgccacc
    25621 agcgccagcg ttgaaagtcc ggcaaagaat ccaacttcaa caccatgttc cggaataatg
    25681 cgcgaggcaa ttgccgcaac cacccacgcg acggcaattt tagttaccac cagcgtaccg
    25741 gatttacgca gtaccgttcc cgtcgcgctt aattttattg acgcccccat gcaaaaaaac
    25801 cacaccgcca gaatgggcac cgtaccggta atcatcccgt tggtgaatga tccaaaatat
    25861 ttccccgccc ccggcgagaa ggtgtggcac agtgcgccaa ggaatagcgg gacgagcatc
    25921 atcccccccg ggattttctc aatcgagcgt tttatctgca tttccatcac ctgtcggcat
    25981 atttttaagg gctgatgcgt aattcctcaa tggagcatca gtgttgatgg aaacgaagat
    26041 agacattcat ttaatcaata aaagtgatca aaaccacatc aattgaaacg ctgttttatt
    26101 tttatcggat cattgttttt attttgaaat tgatcacaaa aaaaccaccg ccgttggcga
    26161 tggttcattt tttaagctga tatgcggcct atacgcctca ttgcagcagg cggcaaatga
    26221 ttattttttc gccgcaaaac gtgccgctgc ttcgtcccag ttcaccacgt tccagaactc
    26281 tttaatgtag tccggacggc ggttctggaa tttcaggtag taagcatgtt cccacacatc
    26341 caggcccaga atcgggaagc cggaagcgcc agaaatagct tcacccatca gcggagaatc
    26401 ctggttagca gtagaaacca ccgccagttt atcgcctttc agcaccagcc atgcccagcc
    26461 ggaaccaaag cgggaagctg ccgctttttc aaattctgct ttgaagttat caacggagcc
    26521 gaagtcacgt tcgatagccg ctttcaggtc accctgcagg gtggtgcctt ttttcagacc
    26581 tttccagaac aggctgtggt tagcgtgacc gccagcgttg ttgcgcagta cggttttctt
    26641 gtctgctggc agctggtcca gtttggtgat cagctcttca accggcaggt tggcaaattc
    26701 tggcaggctt tccagcgccg cgttggcgtt gtttacgtag gtctgatggt gtttggtgtg
    26761 gtggatttcc atggtctgct tatcgaagtg cggttccagg gcatcgtaag catacggcag
    26821 ggatggcagg gtatagctca tattcatctc cagtattgtc gggcggccga ttgttaatgc
    26881 cgcgtaagca gttggttcat tatagttaat taaatgatat tgaaaatgat tatcaatgcc
    26941 gtacttttcg taagggtatg gttttgcagg aaaatgcccg agatgtgaag caaatcaccc
    27001 acttaatgcc gtgattgcca gtaaatcgac aacggcggca acaggcgaaa ggttaatcga
    27061 cagcacgatt tttacactca tctcgtcgga gatgtgaagc gacgaaaaat gatgaggata
    27121 agaagatgag taacgcgatt acgatgggga tattttggca tttgatcggc gcggccagtg
    27181 cagcctgttt ttacgctccg ttcaaaaaag taaaaaaatg gtcatgggaa accatgtggt
    27241 cagtcggtgg gattgtttcg tggattattc tgccgtggac catcagcgcc ctgttactac
    27301 cggatttctg ggcgtattac agctcgttta gtctctctac gctgctgcct gtttttctgt
    27361 tcggcgctat gtgggggatc ggtaatatca actatggcct gaccatgcgt tatctcggca
    27421 tgtcgatggg aattggcatc gccattggca ttacgttgat tgtcggtacg ctgatgacgc
    27481 caattatcaa cggcaatttc gatgtgctga ttaacaccga aggcggacgc atgacgttgc
    27541 tcggcgttct ggtggcgctg attggcgtag ggattgtaac tcgagccggg cagttgaaag
    27601 agcgcaagat gggcattaaa gccgaagagt tcaatctgaa aaaagggctg gtgctggcgg
    27661 tgatgtgcgg cattttctct gccgggatgt cctttgcgat gaacgccgca aaaccgatgc
    27721 atgaagccgc tgccgcactt ggcgtcgatc cactgtatgt cgctctgcca agctatgttg
    27781 tcatcatggg cggcggcgcg atcattaacc tcggtttctg ttttattcgt ctggcaaaag
    27841 tgaaggattt gtcgctaaaa gccgacttct cgctggcaaa accgctgatc attcacaatg
    27901 tgttactctc ggcactgggc gggttgatgt ggtatctgca attctttttc tatgcctggg
    27961 gccacgcccg cattccggcg cagtatgact acatcagttg gatgctgcat atgagtttct
    28021 atgtattgtg cggcggtatc gtcgggctgg tgctgaaaga gtggaacaat acaggacgcc
    28081 gtccggtaac ggtgttgagc ctcggttgtg tggtgattat tgtcgccgct aacatcgtcg
    28141 gcatcggcat ggcgaattaa tctttctgcg aattgagatg acgccactgg ctgggcgtca
    28201 tcccggtttc ccgggtaaac accaccgaaa aatagttact atcttcaaag ccacattcgg
    28261 tcgaaatatc actgattaac aggcggctat gctggagaag atattgcgca tgacacactc
    28321 tgacctgtcg cagatattga ttgatggtca ttccagtctg ctggcgaaat tgctgacgca
    28381 aaacgcgctc actgcacgat gcctcatcac aaaatttatc cagcgcaaag ggacttttca
    28441 ggctagccgc caaccgggta atcagcttat ccagcaacgt ttcgctggat gttggcggca
    28501 acgaatcact ggtgtaacga tggcgattca gcaacatcac caactgcccg aacagcaact
    28561 cagccatttc gttagcaaac gacacatgct gactactttc atgctcaagc tgaccgataa
    28621 cctgccgcgc ctgcgccatc cccacgctac ctaagcgcca gtgtggttgc cctgcgctgg
    28681 cgctaaatcc cggaatcgct ccctgtcagt caagattcag cttcagccgc tccgggcaat
    28741 aaataatatt ctgcaaaacc agatcgttaa cggaagcgta ggagtgttta tcgtcagcat
    28801 gaatataaaa gagatcgcca cgggtaatgc gataaggacg atcgttgagt acatgcagac
    28861 cattaccgcg ccagacaatc accagctcac aaaaatcatg tgtatgttcg gcaaagacat
    28921 cttgcggata acggtcagcc acagcgactg cctgctggtc gctggcaaaa aaatcatctt
    28981 tgagaagttt taactgatgc gccaccgtgg ctacctcggc cagagaacga agttgattat
    29041 tcgcaatatg gcgtacaaat acgttgagaa gattcgcgtt attgcagaaa gccatcccgt
    29101 ccctggcgaa tatcacgcgg tgaccagtta aactctcggc gaaaaagcgt cgaaaagtgg
    29161 ttactgtcgc tgaatccaca gcgataggcg atgtcagtaa cgctggcctc gctgtggcgt
    29221 agcagatgtc gggctttcat cagtcgcagg cggttcaggt atcgctgagg cgtcagtccc
    29281 gtttgctgct taagctgccg atgtagcgta cgcagtgaaa gagaaaattg atccgccacg
    29341 gcatcccaat tcacctcatc ggcaaaatgg tcctccagcc aggccagaag caagttgaga
    29401 cgtgatgcgc tgttttccag gttctcctgc aaactgcttt tacgcagcaa gagcagtaat
    29461 tgcataaaca agatctcgcg actggcggtc gagggtaaat cattttcccc ttcctgctgt
    29521 tccatctgtg caaccagctg tcgcacctgc tgcaatacgc tgtggttaac gcgccagtga
    29581 gacggatact gcccatccag ctcttgtggc agcaactgat tcagcccggc gagaaactga
    29641 aatcgatccg gcgagcgata cagcacattg gtcagacaca gattatcggt atgttcatac
    29701 agatgccgat catgagcgcg tacgaaacag accgtgccac cggtgatggt atagggctgc
    29761 ccattaaaca catgaatacc cgtgccatgt tcgacaatca caatttcatg aaaatcatga
    29821 tgatgttcag gaaaatccgc ctgcgggagc cggggttcta tcgccacgga cgcgttacca
    29881 gacggaaaaa aatccacact atgtaatacg gtcatactgg cctcctgatg tcgtcaacac
    29941 ggcgaaatag taatcacgag gtcaggttct taccttaaat tttcgacgga aaaccacgta
    30001 aaaaacgtcg atttttcaag atacagcgtg aattttcagg aaatgcggtg agcatcacat
    30061 caccacaatg aagctaattg tgaacatcat cacgttcatc tttccctggt tgccaatggc
    30121 ccattttcct gtcagtaacg agaaggtcac gaattcaggc gctttttaga ctggtcgtaa
    30181 tgaaattcag caggatcaca ttatgacctt tcgcaattgt gtcgccgtcg atctcggcgc
    30241 atccagtggg cgcgtgatgc tggcgcgtta cgagcgtgaa tgccgcagcc tgacgctgcg
    30301 cgaaatccat cgttttaaca atggcctgca tagccagaac ggttatgtca cctggaatgt
    30361 ggatagcctg gaaagtgcca ttcgccttgg attaaacaag gtgtgcgagg aagggattcg
    30421 tatcgatagc attgggattg atacctgggg cgtggacttt gtgctgctcg accaacaggg
    30481 tcagcgtgtg ggcctgcccg ttgcttatcg cgatagccgc tccaatggcc taatggcgca
    30541 ggcacagcaa caactcggca aacgcgatat ttatcaacgt agcggcatcc agtttctgcc
    30601 cttcaatacg ctttatcaac tgcgtgcgct gacggagcaa caacctgaac ttattccaca
    30661 cattgctcac gctctgctga tgccggatta cttcagctat cgcctgaccg gcaagatgaa
    30721 ctgggagtac accaacgcca cgaccacgca actggtcaat atcaatagcg acgactggga
    30781 cgagtcgcta ctggcgtgga gcggggctaa caaagcctgg tttggtcgcc cgacgcatcc
    30841 gggtaatgtc attgggcact ggatttgccc gcagggtaat gagattccgg tggtcgccgt
    30901 tgccagccat gataccgcca gcgcggttat cgcctcgccg ttaaacggtt cacgcgctgc
    30961 ttatctctct tctggcacct ggtcattgat gggcttcgaa agccagacgc catttaccaa
    31021 tgacacggcg ctggcagcca acataaccaa tgaaggcggg gcggaaggtc gctatcgggt
    31081 gctgaaaaat attatgggct tatggctgct tcagcgagtg cttcaggagc ggcaaatcaa
    31141 cgatctcccg gcgcttatcg ccgcgacaca ggcacttccg gcctgccgct tcatcatcaa
    31201 tcccaatgac gatcgcttta ttaatcctga ggcgatgtgc agcgaaattc aggctgcgtg
    31261 tcgggaaatg gcgcaaccgc tcccggaaag tgatgctgaa ctggcgcgct gcattttcga
    31321 cagtctggcg ctgctgtatg ccgatgtgtt gcatgagctg gcgcagctac gcggtgaaga
    31381 tttctcgcaa ctgcatattg tcggcggcgg ctgccagaac acgctgctca accagctatg
    31441 tgccgatgcc tgcggtattc gggtgatcgc cgggcctgtt gaagcctcga cgctcggcaa
    31501 tatcggcatc cagttaatga cgctggatga actcaacaat gtggatgatt tccgtcaggt
    31561 cgtcagcacc accgcgaatc tgaccacctt tacccctaat cctgacagtg aaattgccca
    31621 ctatgtggcg cagcttcact cgacacgaca gacaaaggag ctttgcgcat gaccactcaa
    31681 ctggaacagg cctgggagct ggcgaaacag cgtttcgcgg cggtggggat tgatgtcgag
    31741 gaggcgctgc gccaacttga tcgtttaccc gtttcaatgc actgctggca gggcgatgat
    31801 gtttccggtt ttgaaaaccc ggaaggttcg ctgaccggtg ggattcaggc tattggtaat
    31861 tatccgggca aagcgcgtaa tgccagtgag ctacgagccg atctggaaca ggctatgcgg
    31921 ctgattccgg ggccgaaacg gcttaattta catgccatct atctggaatc agacacgcca
    31981 gtctcgcgcg accagatcaa acctgagcac ttcaaaaact gggttgaatg ggcgaaagcc
    32041 aatcagctcg gtctggattt taacccctcc tgtttctcgc atccgctaag cgccgatggc
    32101 tttacgctct cgcatcccga cgacagcatt cgccagttct ggattgatca ctgcaaagcc
    32161 agccgtcgcg tttcggccta ttttggcgag caactcggca caccatcggt gatgaacatc
    32221 tggatcccgg atggtatgaa agatatcacc gttgaccgtc tcgccccgcg tcagcgtctg
    32281 ctggcagcac tggatgaggt gatcagcgag aagctggatc cggcgcacca tatcgacgcc
    32341 gttgagagca aattgtttgg cattggcgca gagagctaca cggttggctc caatgagttt
    32401 tacatggggt atgccaccag ccgccagact gcgctgtgcc tggacgccgg gcatttccac
    32461 ccgactgaag tgatttccga caagatttcc gccgccatgc tgtatgtacc gcagttgctg
    32521 ctgcacgtca gccgtccggt tcgctgggac agcgatcacg tggtgctgct ggatgatgaa
    32581 acccaggcga ttgccagtga gattgttcgt cacgatctgt tcgaccgggt gcatatcggc
    32641 ctcgacttct ttgatgcctc tatcaaccgc attgccgcgt gggtcattgg tacacgcaat
    32701 atgaaaaaag ccctgctgcg tgcgttgctg gaacctaccg ctgagctgcg caagctggaa
    32761 gcggcgggcg attacactgc gcgtctggcg ctactggaag aacagaaatc gttgccgtgg
    32821 caggcggtct gggaaatgta ttgccaacgt cacgatacgc cagcaggtag cgaatggctg
    32881 gagagcgtgc gggcttatga gaaagcgatt ttgagccagc gtgggtaaac actgccggat
    32941 gcggcgcgag cgccttatcc ggcctacagg tcgaatgacc atgcgtgctg ccaccctcac
    33001 cccggccctc tccctgaaag ggcgagggag aaaaacatag cctcgtgcgg caacggagct
    33061 gaacaccgct ccggacagtc ccctcgcccc tacggggaga gggttagggt gaggggaaca
    33121 agccgcacca tgtctaaaca aggcaaccta atttacagga acacagaaca tgcaaaacat
    33181 tacccagtcc tggtttgtcc agggaatgat caaagccacc accgatgcct ggctgaaagg
    33241 ctgggacgag cgcaatggtg gtaacctgac gctgcgcctg gatgacgccg atatcgcgcc
    33301 ttatcacggc aactttcatg cacaaccgcg ctatattccc ctcagccaac ccatgccttt
    33361 actggcaaat acgccgttta ttgtcaccgg ctccggcaaa ttcttccgta acgtccagct
    33421 tgatcctgca gctaacttag gcgtcgtaaa agtcgacagc gacggcgcgg gctaccatat
    33481 tctctggggg ttaaccaacg aagccgttcc aacttccgaa cttccggctc acttcctttc
    33541 ccactgcgag cgcattaaag ccaccaacgg taaagatcgg gtgatcatgc actgccacgc
    33601 caccaacctg atcgccctca cctatgtact tgaaaacgac accgcggtct ttactcgcca
    33661 actgtgggaa ggcagcaccg agtgtctggt ggtattcccg gatggcgttg gcattctgcc
    33721 gtggatggtg cccggcacgg acgaaatcgg ccaggcgacc gcacaagaga tgcaaaaaca
    33781 ttcgctggtg ttgtggccct tccacggcgt cttcggcagc gggtcgacgt tggatgaaac
    33841 attcggttta atcgacaccg cagaaaaatc agcacaagta ttagtgaagg tttattcgat
    33901 gggcggcatg aaacagacca tcagccgtga agagttgata gcgctcggca agcgcttcgg
    33961 cgttacacca ctagctagtg cgctggcgct gtaaggagca aacatgatcc gcaaagcctt
    34021 tgtcatgcag gttaaccccg acgcccacga agagtatcag cgtcggcata accccatctg
    34081 gccggaactg gaagcagtgt tgaaatctca cggtgcgcat aactacgcca tttacctcga
    34141 caaagcgcgt aatctgctgt ttgccacggt agagattgaa tctgaagaac ggtggaatgc
    34201 ggtagccagc actgatgttt gccaacgttg gtggaaatat atgaccgatg ttatgcccgc
    34261 taacccggat aacagcccgg tgagtagcga gctgcaagaa gtgttttacc tgccgtaatt
    34321 gcccaatgtg ccagagatgc ctccggcaca atattttaaa tttcgatttg aaaaattaaa
    34381 catattacat ctcccggtaa gttatatttc cctgatacat tgtgagtaaa tcacaaaaat
    34441 aatgaataac ccattaatga ttcatgtggt ttatttaaat aaccattatg tgcattactc
    34501 cgcaaatctg acctttcact ctgtctcaaa ttgtcattgt ttaccgacat atcgcctctc
    34561 atcacttaat cgctccacca ttacaattga ttaaaatata ttttatggag tccgattaat
    34621 ggcagctctt actgcaagct gtattgacct gaatattcag ggcaatggcg cttattccgt
    34681 tctgaagcag ttggcgacaa tagcgttaca aaacggtttt atcaccgact cacaccagtt
    34741 cctgcaaacc ctgctgctgc gcgaaaaaat gcactctact ggatttggtt ccggtgtcgc
    34801 cgtgccgcac ggtaaaagcg cctgcgttaa acaaccgttt gtattattcg cccgcaaagc
    34861 gcaggctatt gactggaaag ccagcgatgg cgaagacgtc aattgctgga tctgcctcgg
    34921 cgtgccgcaa agcggcgaag aggatcaggt caaaatcatc ggcacactgt gtcgcaaaat
    34981 tatccaccag gattttattc atcaactgca acagggcgac gccgaccagg tgcttgcctt
    35041 gttaaatcaa accctcagct cataaggaag tggcgatgga gtcatcctta tgtattgtcg
    35101 cgatcaccaa ctgccccgcc gggatcgctc acacctacat ggtggcggaa gccctggaac
    35161 agaaagcccg ttctctcggc cataccataa aagtggaaac tcaagggtcc agtggcgttg
    35221 aaaaccgctt atccagcgaa gagattgccg ctgccgatta cgtcattctc gctaccgggc
    35281 gtggcctgag cggtgatgat cgcgcgcggt ttgccgggaa gaaagtttat gagattgcca
    35341 tctcccaggc gttgaaaaat atcgaccaga ttttcagcga attaccgaca aactcgcagc
    35401 tttttgccgc agatagcggc gtgaagcttg gcaaacagga agtgcaaagc ggcagcgtga
    35461 tgagccacct gatggcgggc gtctctgcgg cgctgccgtt tgtcatcggc ggtggtatcc
    35521 tggtggcgct tgccaacatg ctggtgcagt tcggtttgcc gtataccgat atgtcgaaag
    35581 gcgcgccgtc gtttacctgg gtagttgaat ccatcggtta ccttggcttc acctttatga
    35641 tccccatcat gggcgcttat attgcctcgt cgattgccga taaaccggct ttcgctccgg
    35701 cgtttctggt ttgctatctg gcgaacgaca aagcgctgct cggcacccag tcgggtgcgg
    35761 gattcctcgg cgcggtggtg ctggggctgg cgattggcta tttcgtcttc tggttccgca
    35821 aagtgcgcct tggcaaagca ttacaaccgc tgctcggttc aatgctgatc ccgttcgtta
    35881 ctctgctggt tttcggcgtg ctcacttact acgttatcgg gccggtgatg tccgacctca
    35941 tgggcgggct gctccacttc ctgaatacca ttcctccatc aatgaagttt gcggcagcgt
    36001 ttctggtcgg tgcgatgctg gcgttcgata tgggcggccc catcaataaa accgcctggt
    36061 tcttctgctt ctcactgctg gaaaaacaca tttatgactg gtacgccatc gtcggcgttg
    36121 tcgcgttgat gccgcccgtt gcggcgggtc ttgcgacgtt cattgcgccc aaactgttta
    36181 cccgacagga aaaagaggct gccagtagtg ccattgtggt cggtgccaca gtcgcaaccg
    36241 aaccggctat accttacgct ctggcagctc cgttgccgat gattaccgcc aatacgctgg
    36301 cgggtggcat taccggcgtg ctggtgatcg ctttcggaat caaacgtctg gcacctggtt
    36361 tgggtatctt tgacccgctg atcggcctga tgtcgccggt gggttcgttt tatctggtgc
    36421 tagcgattgg cctcgcgctg aacatttcat tcattatcgt cctgaaagga ttgtggttac
    36481 gccgcaaagc gaaagccgcg caacaggagc ttgtccatga acattgagtt actgcaacag
    36541 ttgtgcgaag ccagcgccgt cagcggcgat gaacaggaag ttcgcgacat tctgataaac
    36601 acgctggaac cttgcgttaa tgagatcacc tttgatggtc tgggcagctt tgttgcccgt
    36661 aaggggaata aaggtccaaa agttgccgtt gtcaggcata tggatgaagt cggctttatg
    36721 gtcacccaca tcgacgagag cggttttctg cgctttacca ccattggcgg ctggtggaat
    36781 cagtcaatgc tcaaccaccg ggtaaccata cgcacacaca agggatttaa aatccctggt
    36841 gtgattggtt ccgtcgcacc tcatgcgtta acggaaaagc aaaagcaaca gccgctgtca
    36901 tttgatgaga tgttcattga tattggcgcg aacagtcgcg atgaggtgga aaaacgcggt
    36961 gtggcgatgg gcgattttat tagcccggaa gccaattttg cctgctgggg cgaagataaa
    37021 gtggtcggca aggcgctgga taaccgcatc ggctgcgcaa tgatggctga actattgcag
    37081 acggtgaata atcccgaaat tacgctgtat ggcgtcggca gcgtggaaga agaagttggg
    37141 ctacgcgggg cacaaacctc ggcggaacac attaaaccgg atgtggtgat tgtgctggat
    37201 accgctgtcg caggtgatgt tccgggcatt gataacatta aatacccgct gaaactgggc
    37261 caggggccgg ggctgatgct gtttgacaag cgctacttcc ccaaccagaa actggtggcg
    37321 gcgttaaaaa gctgtgccgc acataacgat ttaccgctgc aattttccac catgaaaacc
    37381 ggtgcgacgg atggcgggcg ctacaacgtg atgggcggcg ggcgtccggt tgttgcgctg
    37441 tgcctgccaa cccgctatct gcacgctaac agcggcatga tttcaaaagc cgattacgat
    37501 gctctgctca cgttgatacg ggattttctg accaccttaa ccgcggagaa agtcaacgcg
    37561 tttagccagt tccgtcaggt ggattaatgc taaacgaacg ccagttaaag attgtcgatc
    37621 tgctggagca acagccgcgc acgcctggtg agctggcgca acagactggc gtttcaggca
    37681 ggaccatcct gcgtgatatt gactatctca actttaccct taacggcaaa gcccgcattt
    37741 tcgccagtgg cagtgcgggc tatcagctgg aaatcttcga gcgccgcagc ttttttcagt
    37801 tgctgcaaaa gcacgataac gacgatcggc tgctggcgct gttattactg aataccttta
    37861 ctccccgtgc gcaactcgcc tcggcgctta atttgccaga aacctgggta gcagagcgtc
    37921 tgccccggtt aaaacagcgt tatgaacgca cttgttgcct ggccagtcgc cccggtttgg
    37981 gccatttcat tgatgagaca gaagagaaac gcgttatctt gctggcgaac ttgctgcgca
    38041 aagatccgtt tttaattccg ctggcgggcg taacacgaga caattttcag catttatcca
    38101 cggcctgcga caaccaacac cgctggccgc tcatgcaggg tgattatctc tccagcctga
    38161 ttctggcgat ttacgccctg cgtaatcaac tgaccgatga gtggccgcaa tatcccggta
    38221 acgagataaa acaaatcgtt gaacagagcg gcatgtttct tggtgataac gctgtaagaa
    38281 ccctgacagg tttgatagag aaacagcatc agcaagcgca ggtaatttca gccgatcatg
    38341 tgctggggtt gctgcaaagg gtgccaggca tcgcgtcatt gaatattatt gatacgcagc
    38401 tggttgagaa tattaccggg catttattac gttgccttgc cgcaccggtg tggattgccg
    38461 agcaccgcca aagcagcatg aataacctga aagccgcctg gcctgcggcc tttgatatga
    38521 gtctgcactt tattacgcta ctgcgtgaac agctcgatat tccccttttc gatagcgatc
    38581 tgatcggttt gtattttgcc tgtgcgctgg aacggcatca aaacgaacgc cagccgatca
    38641 ttttgctctc ggaccagaac gcgattgcca ctattaatca gctcgccatt gagcgcgatg
    38701 ttttacattg tcgggtgatt attgcccgta gcttaagcga gcttattgcc attcgcgaag
    38761 agattgagcc gttattgatc attaacaaca gccattattt actggatgac gcggtaaata
    38821 attacatcac cgtaaaaaat atcattacgg ctgccgatat cgaacaaata aaacactttc
    38881 tggcgacggc atttattcgc caacagccgg aacgcttttt ctctgccccc ggaagttttc
    38941 attattcgaa tgtacgcggt gaaagctggc aacatattac ccggcaaatt tgtgcgcaat
    39001 tagtggcaca acaccatatt accgccgatg aagcgcaacg gatcatcgcc cgcgaaggcg
    39061 aaggtgaaaa cctgattgtt aatcgcctcg ccatcccaca ttgctggagc gaacaggagc
    39121 gacgttttcg tggatttttt attaccctcg cccaaccagt tgaggtgaat aacgaagtca
    39181 ttaaccatgt cttgatcgcc tgcgccgccg ccgatgcgcg tcatgagcta aaaatattta
    39241 gctatctggc aagcgtattg tgtcagcatc cggcagaagt tattgccggg ttaacaggat
    39301 atgaggcatt tatggagtta cttcacaagg ggtgaactgt gtcggatgcg ccgcatccga
    39361 cactgcattt tcatcaaaaa cgtttaacgc aggctattat agtcatcaac catttcgttg
    39421 tactcgcgta acgctcgtgg aaatgagtct tcgaccatcc agcttgtgtt tgcatcctga
    39481 agatgctctt tatcccagtc agagtacggg actttatctc tgacgcggcg aacatatttt
    39541 ttagtctcga gttgatactg gtctgccgaa ttaatgaacg agaaattcat accgccttta
    39601 tccgcttctt tggcctgcgc taccagcgtt tccagttcag agactttctt cattgccgca
    39661 tcaacatcaa
//
LOCUS       DAEEKQ010000020        39409 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.020, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000020 DAEEKQ010000000
VERSION     DAEEKQ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.020"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..266
                     /locus_tag="KLK57_001032"
     CDS             <1..266
                     /locus_tag="KLK57_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HBE0891469.1"
                     /translation="TKAEAPAAQSKAAVPAKASDEEGTRVSINNASAEELARAMNGVG
                     LKKAQAIVSYREEYGPFKTVEDLKQVPGMGNSQVERNLAVLTL"
     gene            360..758
                     /gene="fadM"
                     /locus_tag="KLK57_001033"
     CDS             360..758
                     /gene="fadM"
                     /locus_tag="KLK57_001033"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HBE0891470.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(810..1505)
                     /gene="queC"
                     /locus_tag="KLK57_001034"
     CDS             complement(810..1505)
                     /gene="queC"
                     /locus_tag="KLK57_001034"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HBE0891471.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(1570..3270)
                     /locus_tag="KLK57_001035"
     CDS             complement(1570..3270)
                     /locus_tag="KLK57_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HBE0891472.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQTVTVSELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHRETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPHWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            3370..4188
                     /gene="cof"
                     /locus_tag="KLK57_001036"
     CDS             3370..4188
                     /gene="cof"
                     /locus_tag="KLK57_001036"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HBE0891473.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            4340..4798
                     /gene="decR"
                     /locus_tag="KLK57_001037"
     CDS             4340..4798
                     /gene="decR"
                     /locus_tag="KLK57_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HBE0891474.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            4828..6600
                     /locus_tag="KLK57_001038"
     CDS             4828..6600
                     /locus_tag="KLK57_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBE0891475.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDCVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDVLAQQSGWYRD
                     MYRYQQLEAALDDAPENHEEAVDA"
     gene            6593..8374
                     /locus_tag="KLK57_001039"
     CDS             6593..8374
                     /locus_tag="KLK57_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HBE0891476.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSSNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            8555..8893
                     /gene="glnK"
                     /locus_tag="KLK57_001040"
     CDS             8555..8893
                     /gene="glnK"
                     /locus_tag="KLK57_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HBE0891477.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            8923..10209
                     /gene="amtB"
                     /locus_tag="KLK57_001041"
     CDS             8923..10209
                     /gene="amtB"
                     /locus_tag="KLK57_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HBE0891478.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="KLK57_001042"
     CDS             complement(10258..11118)
                     /gene="tesB"
                     /locus_tag="KLK57_001042"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HBE0891479.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            11336..11908
                     /locus_tag="KLK57_001043"
     CDS             11336..11908
                     /locus_tag="KLK57_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HBE0891480.1"
                     /translation="MKLVHMASCLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPMQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(11939..12250)
                     /locus_tag="KLK57_001044"
     CDS             complement(11939..12250)
                     /locus_tag="KLK57_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HBE0891481.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            12413..12509
                     /gene="ffs"
                     /locus_tag="KLK57_001045"
     ncRNA           12413..12509
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="KLK57_001045"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            12629..12982
                     /locus_tag="KLK57_001046"
     CDS             12629..12982
                     /locus_tag="KLK57_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HBE0891482.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="KLK57_001047"
                     /pseudo
     CDS             complement(13024..14572)
                     /gene="pdeB"
                     /locus_tag="KLK57_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
     gene            complement(14736..15206)
                     /locus_tag="KLK57_001048"
     CDS             complement(14736..15206)
                     /locus_tag="KLK57_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HBE0891483.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSINFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(15322..15873)
                     /gene="maa"
                     /locus_tag="KLK57_001049"
     CDS             complement(15322..15873)
                     /gene="maa"
                     /locus_tag="KLK57_001049"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HBE0891484.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(16044..16262)
                     /gene="hha"
                     /locus_tag="KLK57_001050"
     CDS             complement(16044..16262)
                     /gene="hha"
                     /locus_tag="KLK57_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBE0891485.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="KLK57_001051"
     CDS             complement(16288..16662)
                     /gene="tomB"
                     /locus_tag="KLK57_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HBE0891486.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(17208..20357)
                     /locus_tag="KLK57_001052"
     CDS             complement(17208..20357)
                     /locus_tag="KLK57_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBE0891487.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTSLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="KLK57_001053"
     CDS             complement(20380..21573)
                     /gene="acrA"
                     /locus_tag="KLK57_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HBE0891488.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYLDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            21715..22362
                     /gene="acrR"
                     /locus_tag="KLK57_001054"
     CDS             21715..22362
                     /gene="acrR"
                     /locus_tag="KLK57_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HBE0891489.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            22490..25852
                     /gene="mscK"
                     /locus_tag="KLK57_001055"
     CDS             22490..25852
                     /gene="mscK"
                     /locus_tag="KLK57_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HBE0891490.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="KLK57_001056"
     CDS             complement(26064..26225)
                     /gene="rsmS"
                     /locus_tag="KLK57_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HBE0891491.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPARTVLRALDIVKRDYEKK
                     LTRDDKAEK"
     gene            complement(26239..26766)
                     /gene="priC"
                     /locus_tag="KLK57_001057"
     CDS             complement(26239..26766)
                     /gene="priC"
                     /locus_tag="KLK57_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HBE0891492.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALCHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            26836..27213
                     /locus_tag="KLK57_001058"
     CDS             26836..27213
                     /locus_tag="KLK57_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HBE0891493.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            27366..27917
                     /gene="apt"
                     /locus_tag="KLK57_001059"
     CDS             27366..27917
                     /gene="apt"
                     /locus_tag="KLK57_001059"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HBE0891494.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            28046..29971
                     /gene="dnaX"
                     /locus_tag="KLK57_001060"
     CDS             28046..29971
                     /gene="dnaX"
                     /locus_tag="KLK57_001060"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HBE0891495.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVVPTAVMTPTQVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQCVQGATKAKKSESAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKASAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     AKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    29304..29368
                     /gene="dnaX"
                     /locus_tag="KLK57_001060"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            30024..30353
                     /locus_tag="KLK57_001061"
     CDS             30024..30353
                     /locus_tag="KLK57_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HBE0891496.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            30353..30958
                     /gene="recR"
                     /locus_tag="KLK57_001062"
     CDS             30353..30958
                     /gene="recR"
                     /locus_tag="KLK57_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HBE0891497.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            31068..32942
                     /gene="htpG"
                     /locus_tag="KLK57_001063"
     CDS             31068..32942
                     /gene="htpG"
                     /locus_tag="KLK57_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HBE0891498.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            33123..33767
                     /gene="adk"
                     /locus_tag="KLK57_001064"
     CDS             33123..33767
                     /gene="adk"
                     /locus_tag="KLK57_001064"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HBE0891499.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYFKEAEAGNTKYAKVDGTKSVAEVRADLEKILG"
     gene            33899..34861
                     /gene="hemH"
                     /locus_tag="KLK57_001065"
     CDS             33899..34861
                     /gene="hemH"
                     /locus_tag="KLK57_001065"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HBE0891500.1"
                     /translation="MRQTKIGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEGGSPLMVYSRQQQQALAQRLPETPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(34858..35817)
                     /gene="aes"
                     /locus_tag="KLK57_001066"
     CDS             complement(34858..35817)
                     /gene="aes"
                     /locus_tag="KLK57_001066"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HBE0891501.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQPDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYTLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            35969..37273
                     /gene="gsk"
                     /locus_tag="KLK57_001067"
     CDS             35969..37273
                     /gene="gsk"
                     /locus_tag="KLK57_001067"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HBE0891502.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(37406..39082)
                     /locus_tag="KLK57_001068"
     CDS             complement(37406..39082)
                     /locus_tag="KLK57_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HBE0891503.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
BASE COUNT         9528 a        10049 c        10417 g         9415 t
ORIGIN      
        1 aaaccaaagc ggaagctcct gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg
       61 acgaagaagg cacccgggtc agcattaata atgccagcgc ggaagagcta gcccgcgcga
      121 tgaatggcgt tggcctgaag aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc
      181 cgtttaaaac ggtggaggat ctaaagcagg tgccggggat gggcaattcg caggtggaac
      241 gtaatctggc ggtattaacc ctgtaattaa tttgcatagt ggcaattttt tgccagactg
      301 aagaggtcat accagttatg acctctgtac ttataacaac aacgtaaggt tattgcgcta
      361 tgcaaacaca aatcaaagtt cgtggatatc atctcgacgt ttaccagcac gtcaacaacg
      421 cccgctacct tgaatttctc gaagaagccc gctgggatgg gttggaaaat agcgacagtt
      481 ttcagtggat gacggcccat aacatcgcct tcgtcgtggt caatatcaat attaactatc
      541 gtcgcccagc ggtattaagc gacctgttaa ctattaccag tcagttgcag caattaaacg
      601 gtaaaagcgg catcttaagc caggtcatta cactggagcc ggaagggcag gtggtagcgg
      661 atgcgcttat tacgtttgtt tgtattgatc ttaaaacgca gaaagcatta gctctggaag
      721 gggaattgcg cgaaaagctg gagcagatgg ttaagtaaac gttttgtggt gccggatgct
      781 caagccgcat ccggcgacac ccggaataat tacctcaacc cggttttctg cttcatcgct
      841 gccatcaccg tcggtttatc ggccagataa tgattcaaac cgttggcgcg taaattacat
      901 gccgcacaat gaccgcaacc gtcgccttta atgccgttat agcaggtcaa cgtttcgtta
      961 cggactaaat ccagtttgcc gtaataatct gccagcgccc aggtttccgc tttatcaatc
     1021 cacatcagcg gcgtttcaaa acgaatatct ttcgccatgc ccaaactgac ggcatggttt
     1081 agtgctttca caaactcatc gcggcaatcc gggtagccgg agaaatccgt ttcacagacg
     1141 ccggtaatta cggcttctgc ttttacctga tacgcatata ttgccgccag cgtcaggaac
     1201 aaaatattac gcccagggac aaacgtattc gggatgccat cggcttcagg ttcataatca
     1261 ggcaccggaa tgctgtcacg tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca
     1321 tcaagaacct tatgcgcgcg tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt
     1381 tctgcgcgat gtcgctgacc gtaatcgaac gtcacgcaat ggacttcatc atattgttgt
     1441 aatgcctgca ccagacaggt ggtggaatcc tggcctccac tgaacacaac gacagcacgt
     1501 ttcatagata atcctgcttg aacaataaaa gcgttatggt aacgcctgcg attaacccgg
     1561 accagctatt cattgcgacg gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg
     1621 cggtgtcagt cgcacaccgt tcacgccggg aggggcacta atgcgatagt gatagttgaa
     1681 cagcggcgtc agcgtcgcgt ctgccattaa ctggctaaaa accgctttca gggcattaaa
     1741 tcggttttcc tcatcaggca ttacttgcac cgcgtccagc gtcgattgca gatgtgcata
     1801 tgctggagcg tcgaaaacat gtggccatag tggatcgcaa cgtagccatt gctccagagt
     1861 atattccggt gcttcgccaa ttaatctgtc gcccatcatg aggtctgcgt gtgccagtag
     1921 ggtcgtgtcg tcccagtttt ttgcgttatg aaaaataatt gtgagttcac agccttccgc
     1981 tgccagtgtc gcctgtaggc gttctgccat ggtatgaagt tctatcggta ggtgataaac
     2041 cagcgtcaag gttttcggta gtttgacttc atccggtact tgccagtgcg gaatagtcca
     2101 gcctggcagt aatgcatgac tggcggtgat caggttttct ccgacttcta acgtttgtaa
     2161 taaaccggat tgatgaataa tggagatcac ttttcgcgcc tgccagaggg agagtcgggg
     2221 acttttgcgc aacgttaaat agcaaaaacc taaactgatg ccactactga cctggctgac
     2281 ccgttgcagc tcctccggtt tgccgatggt gatttgcacg ggatgccgac aactggttcc
     2341 caaatctttt tcgaaaagcg gcggagttat ccagtactca accgctttaa gcagcggatg
     2401 acgcaaatgg taataatcat ggctctccag gcgcaccagc tccgctgtga attgtgttaa
     2461 tcgaaaagga ccagtgccga tcagtgggaa ttgcggatgc gccagatggc tgcaatagct
     2521 cgccagccgg tgcgcaagcc agtaatcggg gcgatgtaaa aagaaggtca gacactgcgg
     2581 atgggtgact tcaatacgct tcacgctaat aaataattga tccagtgctg gcagttgtaa
     2641 cagcatcaat aatcgctggt gtaagtgtga ggcttttact gcatcgccgt tatgccagtg
     2701 tagggttgaa cgaagataaa agtcccagcg taacccgtca gtagaggttt ccctgtgatg
     2761 cgctaaatcg ccaatcgggc gctgagtatt attatcgaag cgggtcaggc cggaaaatat
     2821 ctgcccggcg agatgctgct cggcacggcc gggcaaaaag cctggttgta gcggttcgag
     2881 cgggcgatag tagggaatac gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa
     2941 cggctgtaac agagtgcgca gctcacctgg ggccagttgc gccagctcca gcacatcttg
     3001 ctgctttccg gtttccagtg cctgttccat catcgcattg cgtagcgatt ccggcgtgac
     3061 cagaaagcgt aattgtccgc gctttccgcg tcctgactgc gcctgccact ccagccatcc
     3121 cgcctcctgt gcctgacgca acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc
     3181 ggccagttcg ctgacggtga cggtttgcgg ctttccggct gaaggttgcc acagacgttg
     3241 atactggtta agacggttga gcaatcgcat ataaacccgg aacaatatta tttaactatt
     3301 cactattact tccgtatata tcaggtgata ctcaatctcc attaaccgtg tcacagagtg
     3361 gagaaagaaa tggctcgtct ggcagcattt gatatggatg gcactttatt gatgcctgac
     3421 catcatttag gtgagaaaac cctctctact ttggcgcgac tgcgtgaacg cgacattacc
     3481 ctcacttttg ccacggggcg tcatgcgctg gagatgcagc atattctcgg ggcgctatcg
     3541 ctggatgcat atttgattac cggcaacgga acgcgcgtgc attctctgga aggtgaactt
     3601 ttacatcgtg atgatttacc tgcggatgtc gcggagctgg tgctgtatca gcaatgggat
     3661 acccgagcca gcatgcatat cttcaatgac gacggttggt ttaccgggaa agagatccct
     3721 gcgttgttgc aggcatttgt ctatagcggt tttcgttatc agataatcga tgtcaaaaaa
     3781 atgccactcg gcagcgtcac caagatctgc ttctgtggcg atcacgacga tcttacacgc
     3841 ttgcagatcc agctatacga agcattaggc gagcgtgcac atttgtgttt ttccgccaca
     3901 gattgcctcg aagtgctgcc ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc
     3961 caacatttag gtttatcgtt gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc
     4021 gaaatgttag gcagcgtcgg tagcgggttt attatgggca atgcgatgcc gcaactgcgc
     4081 gcggagctcc cgcatttacc ggtgattgga cattgccgaa atcaggctgt ctctcactat
     4141 ttgacgcact ggctggacta tccacatcta ccttattccc ccgaataacg agatcccttc
     4201 cagcaccggg caattgcccg gtttttttgc gttgaatttg tcattttgtg ccgtggtgtt
     4261 taaaccgcac agaataaatt gtcgtgattt cacctttaaa atagaattaa aagagaaaaa
     4321 aattctccgt ggaagggcta tgttagataa aattgaccgt aagctgctgg ccttactgca
     4381 gcaggattgc accctctctt tgcaggcact ggctgaagcc gttaatctga caaccacccc
     4441 ttgctggaag cgcctgaaac ggctggagga cgacggtatc cttatcggca aagtcgccct
     4501 gctggatccg gaaaaaatag gcctcggcct gaccgccttt gtgctgataa aaacgcaaca
     4561 tcacagcagc gaatggtatt gccgctttgt cacggtggtt accgaaatgc cagaagtgct
     4621 ggggttctgg cgcatggctg gtgaatacga ttatctgatg cgcgtccagg ttgccgacat
     4681 gaaacgctac gacgagtttt ataagcgtct ggtaaacagc gtgccggggc tgtcggacgt
     4741 cacttccagc ttcgcgatgg aacagattaa atacaccact tctttaccca tcgaataaat
     4801 atccagaatc aggtcaggac acaacgcgtg cgattatttg ctcaattaag ctggtatttc
     4861 cgtcgggaat ggcgtcgcta tctcggggct gtcgccttgc ttgtcattat cgcgatgctg
     4921 caactggttc cgccaaaagt ggttggtatt gttgtcgatg gcgtgacaga acaacacttt
     4981 actaccgggc tgatcctgat gtggatcgcc accatggtgc tgattgccgt tgtggtttat
     5041 ctcctgcgtt atgtctggcg cgtattgctg tttggcgcgt cttaccaact ggctgttgaa
     5101 ctgcgtgaag attattaccg tcagctaagc cggcagcacc cggagtttta cctgcgtcat
     5161 cgcacgggtg atctcatggc tcgtgcgacc aatgacgtcg attgcgtcgt gtttgccgcc
     5221 ggagaagggg tgctgacgct ggtggattca ctggtaatgg gctgcgctgt gttgattatg
     5281 atgtctacgc aaattagctg gcagttgacc ttatttgccc tgttgccgat gccagtgatg
     5341 gcgatcatga ttaagcgcaa cggcgatgct ttgcatgaac gctttaagct ggcacaggcg
     5401 gcgttttcca gtcttaatga ccgcacccag gaaagcctca ccagtatccg catgatcaaa
     5461 gcctttggtc tggaagatcg ccagtcggcg ttatttgccg cggatgccga agataccggc
     5521 aaaaaaaaca tgcgggtggc gcgtattgat gctcgtttcg acccgaccat ctatatcgcg
     5581 attggtatgg cgaacttgct ggcgattggc ggcggtagtt ggatggtggt gcagggcagt
     5641 ttaacgctgg gccagctcac cagttttatg atgtatttag gtctgatgat ttggccaatg
     5701 ctggcgctgg catggatgtt taacattgtg gaacgtggta gtgctgcgta cagccgtatt
     5761 cgtgcgatgc tggcggaagc gccggtggtg aacgatggta gcgaacccgt gccggaaggg
     5821 cgtggcgaac ttgatgtaaa tattcaccag ttcacgtatc cgcagactga ccatcctgcg
     5881 ctggaaaacg tcaatttcgc cctgaaaccc ggtcagatgc tgggtatctg cgggccgact
     5941 ggttccggca aaagtaccct gttgtcgctc attcagcgtc atttcgacgt tagcgagggg
     6001 gatattcgct ttcatgatat tcctctgacg aagttacaac tcgatagctg gcgtagccgc
     6061 ctggcggtgg tcagccagac gccattcctt ttttctgaca ccgtggcaaa taacatcgcg
     6121 ctgggttgcc cgaatgccac ccagcaagag attgagcatg tcgcgcggtt agccagcgta
     6181 catgacgata ttttgcgttt accgcaaggt tacgatacag aggtgggcga gcgcggcgtg
     6241 atgctttccg gcgggcaaaa acagcgtatc tctattgctc gtgcgttatt agtcaacgcg
     6301 gaaatcctca tccttgatga tgcgctttcg gcggtggacg gacgcacaga gcaccagatc
     6361 ctgcataacc tgcgtcagtg gggacaggga agaacggtaa tcatcagtgc ccatcgcctt
     6421 tcggcactga cggaagccag tgaaattatt gtgatgcagc acggacatat cgcccagcgt
     6481 ggcaatcatg atgtgctggc gcaacaaagc ggctggtatc gcgatatgta tcgctatcaa
     6541 caactggagg cggcgctcga cgacgctccg gaaaatcacg aggaggccgt cgatgcgtag
     6601 ttttagccaa ctgtggccga ctctcaagcg cctgttagcg tacggttcac cgtggcgtaa
     6661 accgctgggg attgcggtcc tgatgatgtg ggttgcggcg gcggcagaag tcagtgggcc
     6721 gctgcttatc agctatttta tcgacaatat ggtagcgaaa aataacctgc cgttgaaagt
     6781 ggttgcaggg ctggctgcgg cgtatgttgg gctacaactg tttgccgccg ggctacatta
     6841 cgcgcagtcg ctgctgttta atcgggcggc agtaggcgta gtgcaacagt tgcgtaccga
     6901 cgtgatggat gctgcgttac gccagccatt aagcgagttt gatacccaac ccgtcgggca
     6961 ggtgatttcc cgcgtcacta atgacactga agtgatccgc gatctctacg ttaccgtagt
     7021 ggcaactgtc ctgcgcagtg ccgcactggt gggcgcgatg ctggtggcga tgttcagcct
     7081 cgactggcga atggcgctgg tggcgataat gattttcccg gtagtgctgg tggtaatggt
     7141 gatatatcaa cgttacagca cgccgattgt ccgtcgtgtg cgcgcctatc tggcggatat
     7201 caacgacggc tttaacgaaa tcatcaatgg catgagcgtc atccagcagt tccgtcagca
     7261 ggcgcgattt ggcgaacgta tgggtgaggc cagtcgttca cactatatgg cgaggatgca
     7321 aaccctgcgc ctcgacggtt ttctgctgcg tccgctgctg agtctgtttt catcgctcat
     7381 tctttgtgga ttgttgatgc tgtttggctt ctccgccagc ggcaccattg aagtgggcgt
     7441 gctgtatgcc tttatcagct atcttgggcg acttaacgaa cccttaatcg aactgaccac
     7501 gcaacaggcg atgctgcaac aggctgttgt tgctggtgag cgcgtgtttg aactgatgga
     7561 cggaccgcgc cagcaatatg gcaatgatga ttacccgtta cagagtggca ccatcgaagt
     7621 cgataacgtg tcatttgctt atcgcgatga caatctggtg ctaaagaaca ttaatctctc
     7681 tgtgccttcg agtaactttg tggcgctggt cgggcatacc ggcagtggta aaagtaccct
     7741 cgccagttta ttgatgggct attacccgct aacggaaggt gagattcgcc ttgatggtcg
     7801 tccattaagt tcgctaagtc acagcgcgct gcgccagggc gtggcaatgg tgcagcaaga
     7861 tccggtggtg ctggcggata ccttcctcgc caacgtgacg ctggggcggg atatctccga
     7921 agaacgcgtc tggcaggcgc tggaaaccgt gcaactggcg gagctggcgc gtagcatgag
     7981 cgacggtatt tacacgccgc tgggcgagca ggggaataat ctctcagttg ggcaaaagca
     8041 actgctggca ctggcgcgcg tgctggtcga gacgccgcaa atcctgatcc ttgatgaggc
     8101 aaccgccagc attgactccg ggactgaaca ggcgattcaa catgctctgg cggcggtgcg
     8161 tgaacatacc acgctggtgg tgattgctca ccgcttatcg accattgttg atgccgacac
     8221 cattctggtg cttcatcgtg ggcaagccgt ggagcagggc actcaccagc aactgctggc
     8281 tgcccaggga cgctactggc agatgtatca actgcaactt gcgggcgaag agctggcagc
     8341 cagcgtgcgt gaagaggaat cattgagcgc ctgaatagcg caatatttca tcgttggtgc
     8401 aaaaatgtaa cgcactgtgc actgtcatag tgcgttttca ttttcaaact tcttaacttc
     8461 ctgctctctt tctcgttttt catttctggc acaccgcttg caataccttc ttcgtgtagc
     8521 agaaccatta ccgaattctg accggagggg atctatgaag ctggtgaccg tgataatcaa
     8581 accattcaag ctggaagacg ttcgtgaagc gttatcttcc attggtattc agggcctgac
     8641 cgtcaccgaa gtgaaaggtt tcgggcgtca gaaagggcat gccgagctgt accggggggc
     8701 ggaatacagc gtcaacttcc tgccaaaagt aaaaattgat gtggcgattg ctgatgacca
     8761 actcgatgaa gtgatcgata tcgtcagtaa ggcggcttac accggaaaaa ttggcgacgg
     8821 caaaatcttc gtcgctgaat tgcaacgcgt cattcgtatt cgtaccggcg aagccgacga
     8881 agcggcgctg taatctctgg cacacagcaa caggaacgaa aaatgaagat agcgacgata
     8941 aaaactgggc ttgcttcact ggcgatgctg ccgggactgg taatggctgc acctgcggtg
     9001 gccgataaag ccgacaatgc gtttatgatg atttgtactg cgctggtgct gtttatgact
     9061 attccgggga ttgccctgtt ttacggtggg ttgattcgcg gcaaaaacgt gctgtcgatg
     9121 ctgacgcagg tgacggtgac gtttgcactg gtctgtattc tctgggtggt ttacggttac
     9181 tcgctggcgt ttggtgaggg caacaacttc ttcggcaaca ttaactggtt gatgctaaaa
     9241 aacatcgaac tgacggcggt gatgggcagc atttatcagt atatccacgt ggcgtttcag
     9301 ggctcgtttg cctgcattac cgtcggcttg atcgttgggg cgctggcgga acgaatccgc
     9361 ttctcagctg tgttgatttt cgtggtggta tggctgacgc tctcttacat tccgattgcg
     9421 catatggtgt ggggcggtgg tttgctggct tctcacggtg cgctggattt cgcgggtggc
     9481 acggtggtgc atattaacgc cgcaatcgcc ggtctggtgg gcgcgtatct gataggcaaa
     9541 cgcgtgggct tcggcaaaga ggcgtttaaa ccgcacaacc tgccaatggt cttcaccggt
     9601 actgccattc tctatattgg ttggtttggc tttaacgccg ggtcagcggg cacggcgaat
     9661 gaaatcgcgg cactggcatt tgtgaatact gtggtcgcaa cggcggcggc aattcttggc
     9721 tggatcttcg gtgaatgggc gctgcgtggt aagccttctc tgctgggagc gtgttctggc
     9781 gcgattgccg gtctggtcgg cgtgacgcca gcgtgtggct acattggggt tggcggcgcg
     9841 ttgattatcg gcgtggtagc tggtctggcg ggcttgtggg gcgttactat gctcaaacgc
     9901 ttgctgcggg tggatgatcc ctgcgatgtc ttcggtgtgc acggcgtttg tggcattgtc
     9961 ggctgtatca tgactgggat ttttgccgcc agttcgctgg gtggcgtggg cttcgctgaa
    10021 ggtgtgacga tgggccatca gttgctggta cagctggaaa gcatcgccat tacgatcgtc
    10081 tggtccggtg tggtggcatt tattggctac aaactggcgg atctgacggt tggtctgcgt
    10141 gtgccggaag aacaggagcg agaagggctg gacgtcaaca gccacggcga gaatgcctat
    10201 aacgcgtaac aagcactgca aaaaacagcc ggacggtttt cacctccggc tattttttta
    10261 attgtgatta cgcatcaccc cttcctgaac ggtcgaggca accagcacgc cgtcctgagt
    10321 ataaaactca ccgcgcacaa agccgcgtgc gctggacgcc gaggtgctct ccacgctata
    10381 cagcagccat tcattcaaat taaacgggcg atgaaaccac atggaatggt caatggtggc
    10441 aatctgaatc cccggttcga gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa
    10501 gttaagatca gaagcgtaac cgagcagata ctgatgaacg cgcaggtcat ccggcacgct
    10561 accatttgcg cggatccaca cctgacgatg tggttctgcg acgtgacctt tcagtgggtt
    10621 atgaaactcc accggacgga cttccagcgg acgatcgcag atgaatttat ctttcagcac
    10681 tggcggcagc aggtgcgcca gcgattgggc gatttgtgtt tccgaaggca ggccatcagg
    10741 cgctggcgcg gacggcattg ttttttgatg ttcgaaaccc gcttcaggtg cctggaaaga
    10801 ggccgtcata taaaaaatcg gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa
    10861 gctgttaccg tcacgcagcg tttcgacatc ataaataatc ggcttcttac tatcgccagg
    10921 gcgaagaaag tagctgtgaa acgaatgtac cagccgctct tcagggacgg tctcttttgc
    10981 agcatacaag gcctgaccca cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc
    11041 actctggccg cgaaagagtc cttcctcaat tttttccaga tttaacaatg tcagtaaatt
    11101 ttttagcgcc tgactcatat aactctccag taactaagct gccgcagcaa gccaaagtga
    11161 gttgagtata acgcaaattt gccactggtc cgatgggtgc aatagtctga attacgggct
    11221 aattacaggc agaaatgcgt gatgtgtgcc acacttgttg acgttactat tttgttaacc
    11281 actcttccgg cgaggaaagt tagcccgctg gtgcattgat aataaggaga agtgaatgaa
    11341 actcgtgcac atggccagtt gtttagcggt tgcgattgcg ttggcggctt gcgcagataa
    11401 aagcgcggat attcagacgc cagcaccggc ggcaaatacg tctatttcag caacacaaca
    11461 acctgctatc cagcaaccga atgtctccgg taccgtttgg atccgtcaga aagtcgcact
    11521 gccgcctgat gctgtgctga ccgtgacact ttctgacgcg tcgttagccg atgcgccgtc
    11581 aaaagtgctg gcgcagaaag cggtgcgtac cgaaggtaaa cagtcaccat tcagctttgt
    11641 tctgcctttt aacccggcag atgttcagcc gaacgcgcgt attctgttga gtgcggcgat
    11701 taccgtgaat gacaaactgg tatttattac cgataccgtt cagccggtga tcaacaaggg
    11761 cggaactaaa gccgacctga cattggtgcc gatgcagcaa accgccgtgc cggttcaggc
    11821 cagcggtggt gcaacgacta ccgtaccttc gacttcacca actcaggtga atccgtcttc
    11881 ggcagttcca gctcctacgc aatattaagc gacgttaacc ctctccgccc ggagagggtt
    11941 agtagttcca gcgataacgc tgcaagtcga tttgcccgct tcccgatacc atcacacctt
    12001 ctgccagtaa tgcctgtcgc tgacgctgta aatccggtcc ggttagcgaa attgtgccgt
    12061 ggcgattaac cacccggtgc cagggtaagg tgctgccttc agggagacgc tttaacacac
    12121 cgcccacctg gcgcgcggcg cggggcgatc ccgccagttt cgccacatca ccgtaagtgg
    12181 tgacatagcc ttcgggaata gcggcgacga tttgccagac gcgttgggga aatgaatctt
    12241 ctttttccat cttttcttcc tgaggtaatt tttcagcata atctggaaaa acgcccgagt
    12301 gaagtcgcat tgcgcaagaa accagcatct ggcacgcgat gggttgcaat tagccggggc
    12361 agcagtgata atgcgcctgc gcgttggttc tcaacgctct caatgggggc tctgttggtt
    12421 ctcccgcaac gctactctgt ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg
    12481 acgcgtgtgc cgggatgtag ctggcagggc ccccacccat ttctgcctcc caccgtttcg
    12541 tcaaaaaatc ccaacatagc taaactttaa ccacaactga cgtcgcaaga attgtctggc
    12601 tgcgcagtac gcttcggagg tatgtctgat gaagtatgtt gatggttttg tggttgccgt
    12661 tcctgccgat aaaaaggatg cctatcggga aatggccgct aaggctgcgc cattgtttaa
    12721 agagtttggc gcgcttcgta ttgtcgaatg ctgggccagc gatgtaccgg atggcaaagt
    12781 gaccgatttt cgtatggcgg tgaaagcgga agagaatgaa gaggttgtct ttagctggat
    12841 tgaataccct tcaaaagagg tccgcgacgc tgctaatcaa aagatgatgt cggacccacg
    12901 gatgaaagag ttcggcgagt ccatgccgtt tgacggcaag cgaatgatct atggcggatt
    12961 cgagtcaatc atcgacgaat aggtagcgtg acgggctgtg cggcgtgccg tcagcccaaa
    13021 aattcacaaa tgttgctcgg cccagcgtaa gaaatcttct ttcggtaatg ccttgctgta
    13081 gagccagccc tgaccgtagt gcacgccatg ctggcgtagc cactcttctt gtttactggt
    13141 ttcgattccc tccgctacca ttttcagttt cagtgttttt gccatttcga tgatatgcgg
    13201 cgtgacattt ttatattcca gcgcatcaac gaaagattta tcgatcttca gaatgtcgac
    13261 atccagatcc tgtaaataac ttaaacttga atatcccgta ccaaaatcat caagataaat
    13321 ttcatggccc gcctcccggt agcgagaaat tatcggggcg ctggttttcg gatcggcaaa
    13381 ctcgcgttca gtgagttcaa gcgcgatctg tctgggatta acctgattga ttgatcattt
    13441 cacgcagcaa ttgcgggatt ttttccgagg tgagcacggt ggattcaaga ttgatcgaaa
    13501 tatgctgctg tggatgctga cgcagccagt cgcccatatc ttcaaaggcg cttcttataa
    13561 tcagtagcgt caatggctca gaaaggcccg tttgctgtgc cagcggaata aaactatctg
    13621 gtgacaacca actaccgtct gtctgcggcc agcgcgccag tgcctcagca ccgacaattt
    13681 tgccattggc taaggagaca atcggctgat agtgcacgca aatatcacga ttttcgatag
    13741 catcctgcag ccgatgatgc ggtgactgga tacggcgcaa aatacgcagc acaaacatcg
    13801 ctgccagcag gccaatcacc aacccgagcg gtaaccaaat aaagacttgc cgatgccagc
    13861 ctttctgcaa cattttcgtt gaagcccagg tgatgatcga aatattcatc tccgggaagg
    13921 gcaggatatc gtaaatgatt ccattatttt cgatatgctc accgggtgtt ttttgtagcc
    13981 tggtaataat tccctgagca atttcatcgc tgctggttat gacaacgtta tgggcattgc
    14041 caataatggc ggcatcaatt tgccatgagc tatagggaat gacatcaata aaggaagcgg
    14101 ggtcgatcat gacgacataa tgtgccgttc ccatggcgac catgtaacgg ataatgccta
    14161 aatcgttatg cgatgttaac cagacacgat aaccatcttt cgaaatttta cctggctcgg
    14221 ggaaggtatc gggcggactt tcatgctcca gagacgaaca ctggggaacg ttgttatcga
    14281 tataaaccac ttcctgaata tagcgataac tgtaagatac ccgacgcatt tccatgagat
    14341 gggcttcgct acaggcagcg ccttgccatc tttccagctc ttgcagcgca tctttccctt
    14401 gtgtcgccac cttattggcc cgaatagcga cgcgggagga ataggtatcc agctcttcaa
    14461 taaacgatgt ttctacctgc tgatgggcca gccagatgct taagccgaca ggcagcaata
    14521 ctgaaagaat cagtactccc gaaataaggc cgaccagatg tcgtgttctc accagcatat
    14581 ccttattaac acagtgtagg gaaagtatat ctgattgtgg aaaaaggctg gcaggagata
    14641 aggaggtaga gctggtattt catgaagtga tgggtggaaa cgatcattgt catttgacca
    14701 agcgcagagc tttacgcctg gtatgtaacc ctaacttaag atgttgattc ggcgcgagcg
    14761 agggtaaaaa tatcgtaaaa agacagttca cctttacgga cgatcatttt ttgcagttgt
    14821 tccttatgaa catccgcgac acgcggagag ttcaagatgg cttcaaccag cgcggcaggt
    14881 acaaagcgcg tgttgtacca ttcgccgtta tagcaaacgc ggaaatccag cacgtcgata
    14941 tcttcatagc ggtagcgtgg gtagacatcg aaaaagaaga aaccattaag caggataaat
    15001 aatacaacca gtagccagac agagcctgac agcgtttcgg actgcaacat caccgccagg
    15061 gtggcaaaaa aagcaacgta cataccgata aacagccccg gatgtttacg gataaaattg
    15121 atactaaagc ggggtttgtt gtcgcgtttt tcgcgggtat tcagagactc aatcgtttcg
    15181 gtcagcaggc gttgtatttc ggtcatcgtt tgccttcgtg gtatctgcaa ctttccaggg
    15241 aacaccctat tttaacgtgg gaatagtaga ggggaagtaa cagatttacc acaattttgc
    15301 ataacagttg cgaaaaaccg attacaattt tttaattatt ctggctggat taccgcccac
    15361 gacaacgttg tccgggacat cttttgtgac aactgcacct gaggctacca cgacgttatc
    15421 accaatggtc acaccagggt taatgaccgc gcgtccgcca atccagacgt tattaccgat
    15481 ggtgacgggt ttccccagtt cagcaccgct attacgtgct acagggtcga tgggatgtgt
    15541 tgccgtgtag atatgaacgc ctggtgccaa catacagtta tcaccgatgc gaatagggca
    15601 gacatcaagc atcacgcaat cgaagttggc gaaaaaatta ttaccgagaa aaatgttata
    15661 gccatagtca cagcgaaacg ttggctcaat ataagcctct gtcacctgac cgaatagatc
    15721 agcgagaatt tgctggcgta atgtgtgctc ttccgccagg gaatgattgt atcggtgaat
    15781 aagctgacga gcgcgcaggc gatcgcgaga taacgtctca tctgccgagc gatacaactc
    15841 accagcaatc atcttttctt tttctgtgct cattactgac ctccggttgc tgaaaatgcg
    15901 gcaacacagt aagacgaaaa gtcagtgaag agaacgttcc cattacatct gtgtgataaa
    15961 gatcacatag ggtatgtctt catggtgaaa agtataaaat tcttaataaa cagccggtta
    16021 tagctccgaa agcgaattat ttattagcga ataaatttcc atactgagga agggatcttg
    16081 tcgtatagtt tattcatggt caattcggcg aggcggtgat ctgcggctga gtaaaatacc
    16141 gccagttcat tatctgataa ttcgtattta tttttctcga taacacgctc cagcgtgtca
    16201 attgtctggc aacgacgtaa acgcattaaa taatcggttt tcgttaaagg tttttcggac
    16261 atacttctac ccatggttgt aataattcta acaagataaa ctcgcaggat tctctttcgt
    16321 cgcattgaca aaacaacgga atagtcgatt acctgacttc cgccatttct gaagatcctg
    16381 catattaata ccataactac tgaacaacat aaaggtgtca tccagatatt cgtcgatctg
    16441 ctcaatgagc ttattgtctt cattatactt aattttgtaa ttaagtgcga aggtctcaat
    16501 atgctcaatc agttcattca actggaggtt gatcgccgag gttgggtcgt taacccagcc
    16561 atgattgctt tcttcaaggt ttgcaaggca gtcatgatac agggtttcac agagaaactt
    16621 aagctgtgcg atatcatgtc ttttgggtga gtattcatcc ataacgcgtc cccttcttag
    16681 cggttgaact aacggacacc tttcgggatg gaaaaaactt actgacctgg acttgccctc
    16741 tcgtttgtta gcttaactat aagccactct ttgcaggttt tcatcgcatt attacgaaaa
    16801 attacaatta ggaaggaatc gggacaggac tcactttttc atactagcac taacccgcag
    16861 caggtaaaag cagtctgctc gaactgttca taacatttca ggattatcgc cagatgaaat
    16921 tggtgacact aataccagga ttgctctgaa tatgacgtaa taaccgagga atgaataaag
    16981 aattaccgca aataattaag aatagcctct aaatgattat ggattattaa atctattagc
    17041 gctacttaat ataaattaac taacaatcag tattttttat gaattttatc cgtggttaat
    17101 actggttttc gtatgagatc ctgagttggt ggttcaatta ctccttaatg ttcgtaggtt
    17161 atgcataaaa aaggccgctt gcgcggcctt agtgattaca cgttgtatca atgatgatcg
    17221 acagtatggc tgtgctcgat atcttcattc ttgcggctaa agcggcggcg aaccaccaca
    17281 aagaataccg gaacgaagaa gattgccagt accgttgcgg tcaccatccc gcccattaca
    17341 ccggtaccta ctgcgttctg cgcgccggaa ccagcaccag tactgataac cagcggcata
    17401 acgccgagga taaacgccag cgaggtcatc aggatcggac gtaaacgcat ccgcactgca
    17461 tcaagcgtcg cttcaatcag acctttacct tctttatcca tcaagtcttt ggcgaattcg
    17521 acgataagta tcgcgttctt cgccgacaac ccaatggttg tgagcaggcc tacctggaag
    17581 taaacgtcat tggtcaggcc acggaaggtg gcagccagca acgcaccgat aacccccagc
    17641 ggaacgacca gcataacgga gaacggaatc gaccagctct cgtacagcgc cgccagacac
    17701 aggaatacga caatcaacga aatcgcgtac agtgaaggtg cctggttgcc ggagagacgt
    17761 tcctgatagg acatccccgt ccagtcatag ccaacaccgg taggcagttt gctcgccagt
    17821 tgttccatca gctccattgc ttcaccggta cttttacccg gtgccgcctg gcctaagatt
    17881 tccatggatg gcaggccgtt gtaacgttcc agacgcggcg aaccgtactc ccaacgagaa
    17941 gaggagaacg ccgagaatgg caccatctga ccatcagcag cacgaacata ccagtcgccg
    18001 atatcatccg gcagcatacg gtatttcgct tctgacatga cgtaaacttt cttcacacga
    18061 ccgcggtcga taaagtcgtt cacatagcta ccgccccatg cagcgcccag agaggtgtta
    18121 atgtcgttga tagaaacacc cagcgcctgc gctttttcct ggtcgatatc aatcttaaac
    18181 tgcggggtat cttccagacc gtttggacgt acgctggtca gcatatcagg gtgcttcgct
    18241 gcttctgcaa gcagctggtt acgcgcctga gtcagttttt cgtgaccaag gccagcctgg
    18301 tcaatcagct caaagtcaaa gccggttgca gtacccagtt ccacgattgc gggcaggtta
    18361 aaggcgaaaa ccatcgcatc tttgatttgc gagaaagcgc gtgttgcacg catggtaatc
    18421 gcttcaactt tgttttcttc gcccggacga tcggcccagt ccttcaagga aacgaacgca
    18481 ataccggtat tctgaccacg tcccgcaaag ccgaagccgt taacggcgaa caccgactca
    18541 acgttgttct tttctttggt cagatagtaa tgcgttacct cattgagcac tttctgcgta
    18601 cgttcctgcg ttgcacctgc tggcagctga accatggtca taaatacgcc ctggtcctca
    18661 tctggcaaga aggagcttgg cagacgcacg aacagatagg ccatgccgac cacgatgatc
    18721 agatacagca ccaggtaacg ccccgtactg cgcagaatac cgcctacgct gtcggtgtag
    18781 tggtgcgtgc tcttctcgaa catgcggtta aaccagccga agaagccttt tttaccttcc
    18841 ccgtgatcgc ctttggcaat cggtttcagc atggtggcac aaagagccgg agtcaggatc
    18901 aacgctacca gtaccgacag cgccattgct gaaacaatgg taatagagaa ctgacgatag
    18961 atagcaccag tagaaccgcc aaagaaggcc atcggtacga ataccgccga cagtaccatc
    19021 gcgataccga ccagagcgcc ctgaatctgc cccatcgact tacgggtagc ttcttttggc
    19081 ggcaaacctt cttccgccat aacacgctcc acgttttcta ccacaacgat agcgtcatcc
    19141 accaacaagc cgatggcgag caccatcccg aacattgtta gcgtgtttat cgagaagcca
    19201 aaggcggcaa ggacggcaaa ggtcccgagc aataccaccg gtacggcaat ggtcggaatc
    19261 aacgtcgcgc ggaagttctg caggaacaga tacataacca ggaacacgag gatgatcgct
    19321 tcgaccagcg ttttaaccac ttcgtgaata gagattttca cgaacggcgt ggtgtcgtac
    19381 gggtaaacaa ttttcagacc cgacgggaag aacggttcca tcttcgccag ttcagcacgg
    19441 attgccgcag cggtatccag cgcgtttgca ccggtcgcca gcttgatccc cagaccggaa
    19501 gccggttggc cgttaaactc tgcgatgatg tcgtagttct caccacccag ctcaatcttc
    19561 gccacatcac gcagcagtac gcgggaacca tcctgattca ctttcagcag gattttgccg
    19621 aactcttcag tagaggtcag acgcgtctga gcaataatag aggcgttaag ctgttggcct
    19681 ttcaccggcg gcgtaccacc gagctgaccc gccgcaacct gggcgttctg cgctttgatg
    19741 gcggtaatga catcaaccgg cgttagctgg aatttgttca gctcattcgg gttcatccag
    19801 atacgcatcg cgtactgtga accgaacaac tgaacatcac ccacgcccga cgtacggctg
    19861 atggcatctt tcatattcgc cgctacgtag tcagagatat cctcctgcgt catggtgcca
    19921 tcggtgttga taacgccgac aaccatcagg aagctgctgg atgatttctc aacgctcacc
    19981 ccttgctgct gaacttcttg cggcagcaac ggcatcgcca gctgcagttt gttctgcacc
    20041 tgaacctgcg cgatatccgc atcagtacca gactcaaagg tcagggtgat ctgcacggta
    20101 cccgtggagt cactgttaga ggacatgtac atcaggttat cgataccgtt catattctgt
    20161 tcgataacct gtgtcaccgt gtcctgcact gttttcgcat cagcgccggg gtaggaggcg
    20221 gagatcgtta ctgccggcgg tgcaatcgta ggatattgcg ccaccggcag tttgaggatc
    20281 gccagccccc ctgccaacat gatgataatg gcgatcaccc acgcaaaaat cgggcgatcg
    20341 ataaagaaat taggcatgtc ttaacggctc ctgtttaagt taagacttgg actgttcagg
    20401 ctgagcaccg cttgcggctt gctggttatt atcagcggta acttcttgtg cttttacctg
    20461 gacaccagga cgcactttct gcagcccact tattactacg cgatcgcctg ctttcagacc
    20521 ttctgtcacc agccacttat cgccaatagc ctggcttgca acgatcggac gggtttccac
    20581 tttgtcatcc gcgccaacta ccagtacggt ggcatcacca cgcggcgtac gggttacgcc
    20641 ctgttgcggg actaaaatag cgtttggatt aagcccttct tccagacgtg cgcgcacgaa
    20701 catacccggc agcagagtgt gatccgggtt cgggaagata gcgcgtaggg tgatagaccc
    20761 agtggtctga tcaacggtaa cgtcagagaa ttccagcgta ccgtcctgcg ggaacttaat
    20821 gccgtcactg gtgatcagcg acactttggc tttgccgttc tcttgtttca gcgtgccatt
    20881 cgccagttcc tgtttcaggc gcaggaagtc gttgctggac tgggtcacat caaggtagat
    20941 cggatcaagt tgctgcacgg ttgccagcgc agtcgcctga ccgttctgta ccaatgcgcc
    21001 ttccgtcacg ttcgacttac caatgcgacc gctaatcgga gaggtgactt tggtgtaagc
    21061 cagattgatc cgcgcagttt caacggcagc tttcgccgca gttaccgcag cattcgcctg
    21121 ttgcgcatca gccagagcct gatcgtactc ttgcttactg atgtactgag taccgagcag
    21181 tttctgataa cgattcaccg tcaattgcgc gatattggct gcagcctggg ctttcgccag
    21241 atcacctttc gcactgtcgt atgtcgcctg ataggtcgca ggatcaatct gatagagaga
    21301 gacaccagct tcgatgtcgc taccttcttt gaaattacgc ttcaggataa tcccgctaac
    21361 ttgaggacga acttctgcga tccggtaggc actggtgcga cccggaagct cggttgtgat
    21421 ctgcagaggt tcagttttga ctgttactac gccaacggcg ggcatctgct ggccaccttg
    21481 ttgggcctgt ttgtcgtcac atcctgttag ggctaagctg cctgagagca tcagaacgac
    21541 cgccagaggc gtaaaccctc tgtttttgtt catatgtaaa cctcgagtgt ccgatttcaa
    21601 attggtcaat ggtcaaaagt taataaaccc attgctgcgt ttatattatc gtcgtgctat
    21661 ggtacataca ttcacaaatg tatgtaaatc taacgcctgt aaattcacga acatatggca
    21721 cgaaaaacca aacaagaagc gcaagaaacg cgccaacaca tcctcgatgt ggctctacgt
    21781 cttttctcac agcagggggt atcatccacc tcgctgggcg agattgcaaa agcagctggc
    21841 gttacgcgcg gtgcaatcta ctggcatttt aaagacaagt cggatttgtt cagtgagatc
    21901 tgggaactgt cagaatccaa tattggtgaa ctagagcttg agtatcaggc aaaattccct
    21961 ggcgatccac tctcagtatt aagagagata ttaattcatg ttcttgaatc cacggtgaca
    22021 gaagaacgac gtcgattatt gatggagatt atattccaca aatgcgaatt tgtcggagaa
    22081 atggctgttg tgcaacaggc acaacgtaat ctctgtctgg aaagttatga ccgtatagaa
    22141 caaacgttaa aacattgtat tgaagcgaaa atgttgcctg cggatttaat gacgcgtcgg
    22201 gcagcaatta ttatgcgcgg ctatatttcc ggcctgatgg aaaactggct ctttgccccg
    22261 caatcttttg atcttaaaaa agaagcccgc gattacgtcg ccatcttact ggagatgtat
    22321 ctcctgtgcc ccacgcttcg taatcctgcc actaacgaat aaccctgaat ctgactccag
    22381 gatttttcct ggacattttc gtcgttgcta ttctggttca ctgcgtcgtg atattcttgc
    22441 ggtttgactt tttcaggtcg ttcttcaggt tcagaaacct tcattcatca tgactatgtt
    22501 ccagtattac aaacgatcac ggcattttgt tttttcagca tttattgctt ttgtttttgt
    22561 cttgttatgc cagaacacgg cgtttgcgcg ggcgtcatcg aatggtgatc tgccgacaaa
    22621 agcggacctg caggcgcaac ttgactcact aaataaacaa aaagatcttt ctgctcagga
    22681 caaactggtg cagcaggatc tgacagatac attagccacc ctcgataaaa tcgatcgcat
    22741 aaaagaagag acagttcagc tacggcaaaa agtcgctgaa gcgccagaaa aaatgcgcca
    22801 ggcgaccgcg gcgttaacag cacttagcga tgtcgataac gacgaagaaa cgcgcaaaat
    22861 tctgagtacg ctttcgttgc gccagctgga aactcgcgtt gcccaggcgc tggacgattt
    22921 gcaaaacgca caaaacgatc tggcgtctta taacagccag ctggtttcgt tacagacgca
    22981 gcccgaacgc gtgcaaaatg cgatgtataa cgcttcgcag cagctgcaac aaattcgcag
    23041 tcgtctggat gggactgatg tcggcgagac tgccttacgt cccagccaga aagtgttaat
    23101 gcaggctcag caggcgttgc tgaatgcgga gattgaccag cagcgtaaaa gcctggaagg
    23161 gaacaccgtc ttgcaggata ccttgcaaaa gcaacgtgat tacgtgacgg cgaacagcgc
    23221 ccgtctggag caccagttgc agctgttgca agaagcggta aacagcaagc gcctgacttt
    23281 aactgaaaaa acggcgcagg aagccgtctc cccggatgaa gccgcgcgta ttcaggctaa
    23341 tccgctggtg aaacaggaac tggaaattaa ccagcagtta agtcagcgtc tgattaccgc
    23401 gactgaaaac ggtaatcagt tgatgcagca aaacattaaa gtcaaaaact ggctggagcg
    23461 ggcgctgcaa tcggaacgca atattaaaga gcagattgcc gtcctgaagg gcagcctgct
    23521 gttgtctcgt atcctttacc agcaacaaca aacgctgccc tcggcggatg aactggaaaa
    23581 catgaccaac cgcatcgcgg atttgcgtct cgaacagttt gaagttaacc agcagcgtga
    23641 tgcactcttc cagagcgatg cgttcgtcaa caaactggaa gaaggtcaca ccaacgaagt
    23701 caacagcgaa gttcacgatg cgttattgca agtggttgat atgcgtcgcg aattgctgga
    23761 tcaactcaac aaacagttgg gtaaccagct gatgatggcc attaacctgc aaatcaacca
    23821 gcagcagtta atgagcgtat cgaaaaacct gaaatccatc ctgactcagc aaatcttttg
    23881 ggtgaacagt aaccgtccaa tggactggga ctggatcaaa gcgttcccgc aaagcctgaa
    23941 agatgaattt aagtcgatga aaatcacggt gaactgggaa aaagcctggc ctgccgtttt
    24001 tatcgctttc ctcgccggtt tgccgctgct gttgattgcc gggctgatcc actggcgtct
    24061 gggctggctg aaagcgtatc aacagaaact ggcttccgct gtgggttccc tgcgtaacga
    24121 tagccagctc aacacaccaa aagcgatcct tatcgacctg atccgcgcgc tgccggtgtg
    24181 cctgattatt ctcgcggttg gcctgattct gttgaccatg cagctcaaca tcagcgaact
    24241 gctatggtcg ttcagtaaaa aactggcgat tttctggctg gtgtttggcc tgtgctggaa
    24301 ggtactggag aaaaatggcg ttgccgtgcg tcacttcggc atgccggagc agcagaccag
    24361 ccactggcgt cggcaaattg tccgcatcag tctcgcgttg ctgcctatcc atttctggtc
    24421 tgtggtggca gaactttccc cgctgcatct gatggatgat gtgctggggc aggcgatgat
    24481 tttcttcaac ctgctgctga ttgccttcct ggtatggcca atgtgccgcg aaagctggcg
    24541 tgataaagag tcgcacacca tgcgactggt caccattacc gtgctgtcga ttatcccgat
    24601 tgcattgatg gtgctgactg ccacgggtta tttctacacc acgctgcgcc tctccgggcg
    24661 ttggattgaa accgtttatc tggtgatcat ctggaacttg ctctaccaga cggtattgcg
    24721 cggcttaagc gtagcggcgc ggcgtatcgc ctggcgtcgt gcgctggcgc gtcggcagaa
    24781 tctggtgaaa gagggcgcag aaggtgccga accgccggaa gaacccacca ttgcactgga
    24841 acaggttaac cagcagacgc tgcgtattac catgttgctg atgtttgcgc tgttcggtgt
    24901 catgttctgg gcaatttggt ccgatttgat caccgtgttc agctatctcg acagcatcac
    24961 gctctggcat tacaacggca ctgaagctgg cgctgcggtg gtgaaaaacg tcaccatggg
    25021 cagtctgttg tttgcgatta tcgcctcaat ggtggcctgg gcgttgattc gcaacctgcc
    25081 tggtttactg gaagtgctgg tgctctcgcg actgaatatg cgtcagggcg cgtcgtatgc
    25141 cattactacc atccttaact acatcattat tgccgttggt gcgatgacgg tgttcggatc
    25201 gctgggcgtc tcttgggata aactccagtg gctggccgca gcattatccg taggtcttgg
    25261 ttttggttta caagaaattt tcggtaactt cgtctccggt ttgatcattc tgttcgaacg
    25321 tccggtgcgt attggcgata cggtaaccat tggtagcttc tcggggacgg taagtaagat
    25381 ccgtattcgt gcgacaacga ttaccgattt cgatcgcaaa gaagtgatca tcccgaacaa
    25441 agcgtttgtt accgagcgtc tgatcaactg gtcgttgact gacactacta cgcgtctggt
    25501 gatccgtctc ggcgtggcct atggctccga tctggaaaaa gtgcgtaaag tgttactgaa
    25561 ggcggcgact gagcacccaa gggtgatgca cgaaccaatg ccggaagtct tctttacggc
    25621 gtttggtgcc agcacgttgg atcatgagct gcgtctgtat gtgcgtgaac tgcgtgaccg
    25681 tagtcgtact gtcgatgagc tgaaccgtac tatcgatcag ctgtgccgtg aaaacgacat
    25741 caacattgcc tttaaccagc ttgaagtgca tctgcacaac gagaagggcg atgaggtgac
    25801 ggaagtcaaa cgcgactaca aaggcgatga cccgacgcca gcggtagggt aataaccaaa
    25861 gggggcgaca tttagttgcc ccgagattac tgacaaagtg cgcgttgttc atgccggatg
    25921 cggcgtgacc gccttatccg gcctacgaaa ccgcaagaat tcaatgtatt gcaggagcgg
    25981 tgtaggcctg ataagcgtag cgcatcaggc agttttgcgt ttgcccacaa tcttagggga
    26041 catttagcgg ccccatttat ttctcacttt tccgccttat catcgcgcgt taatttcttt
    26101 tcataatcgc gttttacaat atccagcgcg cgcagcacgg tgcgggctgg gatctgattt
    26161 tcttccagca gcacaatcaa atcgacagcc agtttgacat cgtcaggggc attttcaagt
    26221 gacatactct ctccattgct agcgggttaa gcgcgctagc ctgttttcga ttttttccag
    26281 cgcatggcgg cagcgtgcca ggcgcgcttc ataggcttcc acttcacgat gcagcgtttg
    26341 ctgttccacg agatcggtca cccgcgccag gcgggctctg cgttcggcaa ccatctcacg
    26401 tagccgccgc tcaaaatcct gatgctgaat acgtttacgc tgccagcggg caattttcgg
    26461 tggcgcactg tcccactcgc gcaatgacca ggcggaggct tcacgcgcga tggcttccag
    26521 ttgtgccgcc agatgttccg ccagccaggc cacttgcggc agctgttgct gctcaaccgc
    26581 atgacaaagc gcagccagat tatcgcccgc ctcgtcgaga caagcctgta gtgttgtcgc
    26641 acgagtctga aaaagatgcc tgtcgaaacg agcacttagc gtggcgaact gtgacaccgg
    26701 ggcacaacgc tgacgcagcg tagcgagctg tccttccagt ttttccagca gcagggcggt
    26761 tttcacgata agacctcaat gaaaatgata attgttatgc taaagtagcc actctgtaag
    26821 ctgacaacac tcaatatgca acgaatcatt ttaatcatca ttggctggct ggcggtagtg
    26881 ctgggtacgc tgggcgtggt attaccggta ttaccgacga cgccgtttat cctgctggcg
    26941 gcctggtgct ttgcccgttc ttccccgcgc tttcacgcct ggttgctgta ccgctcatgg
    27001 tttggcagct atctacgttt ctggcagaaa catcatgcga tgccgcgcgg cgtcaaaccg
    27061 cgggcgattt tgcttatttt gctcacgttt gccatttctc tgtggttcgt ccagatgcca
    27121 tgggtgcgca tcatgttgct ggtaattctc gcctgtttgc ttttctatat gtggcgaatt
    27181 ccggtgattg atgaaaagca agaaaagcac tgaagcacaa caatcgcagt tgcaattatt
    27241 gcgtacagcc agtacattct ggcgttttcg agcacaggcg caggcggtca aaggttaaac
    27301 aactgttact tttgatacgt ttaaaacgcg ccgtgagtac caccgtaaca agcaggcata
    27361 cacttatgac cgcgactgca cagcagcttg agtatctcaa aaatagcatc aaaagcattc
    27421 aggactaccc aaaacccggc attcttttcc gcgatgtcac cagcttactg gaagacccga
    27481 aagcttacgc tctcagcatc gacttgctgg ttgagcgtta caaaaatgcg ggcattacca
    27541 aagttgtcgg caccgaagcg cgtggcttct tgtttggcgc tccggtagct ctgggtctgg
    27601 gcgttggctt tgtaccggtc cgtaaaccgg gcaaactgcc gcgtgaaacc atcagtgaaa
    27661 cttacgacct ggaatacggc accgatcagc tggagatcca cgttgatgcc atcaaaccgg
    27721 gcgacaaagt tctggtggtg gacgatctgc tggcaaccgg cggcactatc gaagcgaccg
    27781 ttaaactgat ccgtcgtctg ggtggtgaag tggctgacgc tgcgttcatt atcaacctgt
    27841 tcgatctcgg cggcgaacag cgtctcgaaa aacagggcat taccagctac agccttgtcc
    27901 cgttcccggg ccattaatta tcgccagtct tgtgctgccc acgctacgga cagcacaaga
    27961 tgtgcattca gcctcgccgt tctgacgggg ctgtgttagc attacccctt cgtgaatcca
    28021 ccttccagcg tttcagagcc tgccaatgag ttatcaggtc ttagcccgaa aatggcgccc
    28081 acaaaccttt gctgacgtcg tcggccagga acatgtgctg accgcactgg cgaacggctt
    28141 gtcgttaggg cgtattcatc atgcttatct tttttccggc acccgtggcg tcggaaaaac
    28201 ctctatcgcc cgactgctgg cgaaggggct aaactgcgaa accggcatta ccgcgacgcc
    28261 gtgcggcgtg tgcgataact gtcgtgaaat cgagcagggg cgctttgtcg atctgattga
    28321 aatcgacgcc gcctcgcgca ccaaagttga agatacccgc gacctgctgg ataacgtcca
    28381 gtacgctccg gcgcgtggtc gtttcaaagt ttatctgatc gacgaagtgc atatgctgtc
    28441 gcgccacagc tttaacgcac tgttaaaaac ccttgaagag ccgccggagc acgttaagtt
    28501 tctgctggcg acgaccgatc cacagaaatt gccggtgacg attttgtcgc gctgtttgca
    28561 atttcatctc aaggcgctgg atgtcgagca aattcgccat caacttgagc acatcctcaa
    28621 cgaagaacat atcgctcacg agccgcgggc gttgcaattg ctggcgcgcg ccgctgaagg
    28681 cagcctgcga gatgccttaa gtctgaccga ccaggcgatt gccagcggtg acggccaggt
    28741 ttcaacccag gcagtcagtg cgatgctggg tacgcttgat gacgatcagg cgctgtcgct
    28801 ggttgaagcg atggtcgagg ccaacggcga gcgcgtaatg gcgctgatta atgaagccgc
    28861 tgcccgtggt atcgagtggg aagcgttgct ggtggaaatg ctcggcctgt tgcatcgtat
    28921 tgcgatggta caactttcgc ctgctgcact tggcaacgac atggccgcca ttgagctgcg
    28981 gatgcgtgaa ctggcgcgca ccataccgcc gacggatatt cagctttact atcagacgct
    29041 gttgattggt cgcaaagaat taccgtatgc gccggaccgc cgcatgggcg ttgagatgac
    29101 gctgctgcgc gcgctggcgt tccatccgcg tatgccgctg cctgagccag aagtgccacg
    29161 ccagtccttt gcacccgtcg tgccaacggc agtaatgacg ccaacccagg tgccgccgca
    29221 atcagcgccg cagcaggcac cgactgtacc gctcccggaa accaccagcc aggtgctggc
    29281 ggcgcgccag cagttgcagt gcgtgcaggg agcaaccaaa gcaaaaaaga gtgaatcggc
    29341 agccgctacc cgcgcgcggc cggtgaataa cgctgcgctg gaaagactgg cttcggtcac
    29401 cgatcgcgtt caggcgcgtc cggtgccatc ggcgctggaa aaagcgtcag ccaaaaaaga
    29461 agcgtatcgc tggaaggcga ccactccggt gatgcagcaa aaagaagtgg tcgccacgcc
    29521 gaaggcgctg aaaaaagcgc tggaacatga aaaaacgccg gaactggcgg cgaagctagc
    29581 ggcagaagcc attgagcgcg acccgtgggc ggcacaggtg agccaacttt cgctaccaaa
    29641 actggtcgaa caggtggcgt taaatgcctg gaaagaggag agcgacaacg cagtatgtct
    29701 gcatttgcgc tcctctcagc ggcatttgaa caaccgcggt gcacagcaaa aactggctga
    29761 agcgttgagc atgttaaaag gttcaacggt tgaactgact atcgttgaag atgataatcc
    29821 cgcggtgcgt acgccgctgg agtggcgtca ggcgatatac gaagaaaaac ttgcgcaggc
    29881 gcgcgagtcc attattgcgg ataataatat tcagaccctg cgtcggtttt tcgatgcgga
    29941 gctggatgaa gaaagtatcc gccccatttg atcgtaagca cagcttacgt tcgtcatcct
    30001 taacgtgatt gagagagaaa cctatgtttg gtaaaggcgg tctgggtaac ctgatgaagc
    30061 aagcccagca gatgcaagaa aaaatgcaga aaatgcagga agagatcgcg cagctggaag
    30121 tcaccggcga atctggcgca ggtctggtaa aagtgaccat caacggtgca cacaactgcc
    30181 gtcgcgtaga gatcgacccg agcctgctgg aagacgacaa agagatgctg gaagacctgg
    30241 tggctgcagc attcaacgac gcagcacgtc gtattgaaga aacgcagaaa gaaaaaatgg
    30301 cctctgtatc ctccggaatg cagctgccgc ctggctttaa gatgccgttc tgatgcaaac
    30361 cagcccgctg ttaacacagc ttatggaagc actgcgctgt ctgccgggcg ttggcccgaa
    30421 gtcggcgcag cgtatggcgt tcacgctgct tcagcgcgat cgtagcggcg ggatgcgttt
    30481 ggcgcaggcg ctcacccggg cgatgtcgga aatcggccac tgcgccgatt gccgcacttt
    30541 caccgaacag gaagtctgta acatctgttc gaatccgcgt cgtcaggaaa acggtcaaat
    30601 ctgtgtggtg gagagtccgg cggacatcta cgccattgag cagacggggc agttttctgg
    30661 tcgttatttt gtgttgatgg ggcatctgtc accgctggac ggcatcggtc cggatgatat
    30721 cgggcttgat cgtctggaac agcgtctggc agaggaaaaa atcactgaag tgatcctcgc
    30781 caccaatccc acggttgaag gtgaagctac cgctaactac attgccgagc tttgcgcgca
    30841 atatgacgtg gaagccagcc gaatcgctca tggcgtaccg gttggcggcg aactggaaat
    30901 ggtcgatggc accacgctgt cacactccct tgccgggcgt cataagattc gtttttaagc
    30961 aaacgagagc aggatcacct gctctcgctt gaaattattc tcccttgtcc ccatctctcc
    31021 ctcatcctgt ttttaacctt aaaatggcat tattgaggta gacctacatg aaaggacaag
    31081 aaactcgtgg ttttcagtca gaagtgaaac agcttctgca cctgatgatc cattctctct
    31141 attccaataa agaaatcttc ctgcgtgagc ttatctctaa cgcctccgat gcggcggaca
    31201 agctgcgttt ccgtgcgcta tctaacccgg acctgtacga aggtgatggc gaactgcgcg
    31261 ttcgtgtctc tttcgataaa gacaagcgca cgctgaccat ctctgataac ggcgtgggga
    31321 tgacccgcga cgaagtgatt gaccatctgg ggactatcgc taaatccggt accaaatcat
    31381 tcctcgaatc cctgggttct gaccaggcga aagacagcca gctgatcggt cagtttggtg
    31441 ttggtttcta ctctgcgttt atcgtggccg acaaagtgac cgtgcgtact cgcgcggcag
    31501 gcgaaaaacc agaaaacggc gtcttctggg aatcggctgg cgaaggtgaa tataccgttg
    31561 ccgacatcac caaagaagat cgtggtactg aaatcaccct gcatctgcgt gaaggcgaag
    31621 acgagttcct cgatgactgg cgcgtgcgtt ccatcatcag caaatactcc gaccatatcg
    31681 cgctgccggt agagatcgaa aaacgcgaag agaaagacgg cgaaaccgtt atctcctggg
    31741 agaaaatcaa caaagcgcag gcgctgtgga ctcgtaacaa gtcggaaatc accgatgaag
    31801 agtacaaaga gttctacaaa cacatcgccc acgactttaa tgatccgctg acctggagcc
    31861 acaaccgcgt tgaaggtaag caggagtaca ccagcctgct gtacattccg tcccaggctc
    31921 cgtgggatat gtggaaccgt gatcataaac acggcctgaa actgtacgtc cagcgtgtgt
    31981 tcatcatgga cgacgcagaa cagttcatgc cgaactatct gcgcttcgtg cgtggtctga
    32041 ttgactccag cgatctgccg ctgaacgttt cccgtgaaat cctccaggac agcacggtaa
    32101 cgcgtaacct gcgcaatgcg ctgaccaagc gtgtgctgca aatgctggaa aaactggcga
    32161 aagacgacgc ggaaaaatac cagaccttct ggcaacagtt tggcctggta ctgaaagaag
    32221 gtccggcgga agatttcgct aaccaggaag cgatcgccaa actgctgcgt tttgcttcta
    32281 cccataccga ttcttctgcg cagaccgtat ctctggaaga ctacgtttcc cgcatgaaag
    32341 aagggcagga gaaaatctac tacatcaccg cagacagcta tgcggcagcg aagagcagcc
    32401 cgcacctgga actgctgcgt aagaaaggca tcgaagttct gctgctttcc gaccgcatcg
    32461 atgagtggat gatgaactat ctgactgagt tcgacggtaa accgttccag tcggtgtcta
    32521 aagttgacga gtcgctggaa aaactggctg acgaagttga tgagagcgcg aaagaagcgg
    32581 agaaagcact gactccgttc atcgaccgtg tgaaagccct gctcggcgag cgcgtgaaag
    32641 atgtccgtct gactcaccgt ctgaccgata cgccagcgat cgtttcgacc gacgcggacg
    32701 aaatgagcac tcagatggcg aaactgttcg ctgcggcggg ccagaaagtg ccagaagtga
    32761 aatacatctt cgaactgaac ccggatcacg tactggtgaa acgtgcggca gatactgaag
    32821 atgaagcgaa gttcagcgag tgggtagaac tgctgctgga tcaggcgctg ctggcagaac
    32881 gcggcacgct ggaagatccg aacctgttta ttcgtcgtat gaaccagctg ctggtttcct
    32941 gatgtcatgc cggatgacct ttgtgtcatc cggcattttt cttttcatca tctgcacttt
    33001 ccgcaaatta tctcgccatt aaccgtttca gcctcaggtg cctttcttga ggcaatcgcc
    33061 tgttggtggt atcgtttatc gctttttcaa aaaaatcgac acattttaag gggattttcg
    33121 caatgcgtat cattctgctt ggcgctccgg gcgcggggaa agggactcag gctcagttca
    33181 tcatggagaa atatggtatt ccgcaaatct ccactggcga tatgctgcgt gctgcggtca
    33241 aatctggctc cgagctgggt aaacaagcaa aagacattat ggatgctggc aaactggtca
    33301 ccgacgaact ggtgatcgcg ctggttaaag agcgcattgc tcaggaagac tgccgtaatg
    33361 gtttcctgtt ggacggcttc ccgcgtacca ttccgcaggc agacgcgatg aaagaagcgg
    33421 gcatcaatgt tgattacgtt ctggaattcg acgtaccgga cgaactgatt gttgatcgta
    33481 tcgtaggccg ccgcgttcat gcgccgtctg gtcgtgttta tcacgttaaa ttcaatccgc
    33541 cgaaagtaga aggcaaagac gacgttaccg gtgaagaact gactacccgt aaagacgatc
    33601 aggaagaaac cgtgcgtaaa cgtctggttg aataccatca gatgactgca ccgctgatcg
    33661 gctactactt caaagaagcg gaagcgggta acaccaaata cgcgaaagtt gacggcacca
    33721 agtcggttgc tgaagttcgc gctgatctgg aaaaaatcct cggctaatca agccgcagca
    33781 gatctgcttt cgggcaggtc tgctcatcca cctcaggcaa tacctctcac agcaattagt
    33841 tcttcttcct cacttttccg ctacaattat caacaagttg aatcgataag aggcggtaat
    33901 gcgtcagact aaaatcggta tcctgctggc aaacctgggt acgcccgatg cccccacacc
    33961 tgaagcggta aaacgctatc tgaaacaatt tttaagcgac agacgcgtgg ttgatacctc
    34021 acggttgtta tggtggccat tgctgcgcgg cgttattttg ccgctgcgct cgccgcgtgt
    34081 ggcgaagctg tacgcctcgg tctggatgga aggtggctcg ccgctgatgg tttacagccg
    34141 ccagcaacag caggcgctgg cacaacgttt accggagacg cccgtagcgc tgggaatgag
    34201 ctacggctcg ccatcactgg aaagcgccgt cgatgaactc ctggcagagc atgtagatca
    34261 tattgtggtg ctgccgcttt atccgcaatt ctcctgttct acggtcggtg cggtatggga
    34321 tgaactggca cgcattctgg cgcgcaaacg tagcattccg gggatatcgt ttattcgtga
    34381 ttacgccgat aaccacgatt acattaatgc actggcgaac agcgtacgcg cttcttttgc
    34441 caaacatggc gaaccggatc tgctgctgct ctcttatcat ggcattcccc agcgttatgc
    34501 agatgaaggc gatgattacc cgcaacgttg ccgcacaacg actcgcgaac tggcttccgc
    34561 actggggatg gcaccggaaa aagtgatgat gacctttcag tcgcgctttg gccgggaacc
    34621 ctggctgatg ccttataccg acgaaacgct gaaaatgctc ggagaaaaag gcgtaggtca
    34681 tatacaggtg atgtgcccgg gctttgctgc ggattgtctg gagacgctgg aagagattgc
    34741 cgagcaaaac cgtgaggtct tcctcggtgc gggcgggaaa aaatatgaat atattccagc
    34801 gcttaatgcc acgccggaac atatcgaaat gatggctaat cttgttgccg cgtatcgcta
    34861 aagctgagcg gtaaagaact gagcgccgtc gcgaagagcc tcgtcggcgg ttttcatcat
    34921 ccgtgaataa tgcaaaaagg catgcagcgt acctgggtag agtttgaact cacagggctg
    34981 ctgatgcgcc gctaacgtct ggtaaagcag acggctgtca tccagcagcg gatcgaactc
    35041 cgccccggca ataaaacagg gcggaacttc gcgagtgaga tcattattaa acagacagta
    35101 atacggtgac tcgcggtccg cgtcgttgct taaatacgcc tcttcataca tctgcaaatc
    35161 ctgttgcgtt aagccatccc agacaccgcc caacagacga cgagtcacgg aatcccgtaa
    35221 tccgtaaagc ccataccaca gcaaaacacc cgcaatttta ccgcaatcga tctgtttatc
    35281 acgcaaccac aacgcactgg cgagcgccag catggcacct gcggaatcac cggcaaagcc
    35341 aatgcgggac atattgattt gataatcctc cgcctgctgg tggaaataac aacaagcagc
    35401 cacaatttcc tctatcgctt gcggaaaacg cgcttcaggt gaaagggtgt aatcaatacc
    35461 aatcaccgta cattggctgt agcttgccag caggcgcatg atgcgatcgt gggtatcgag
    35521 attgccgaga ataaaaccgc ctccatgcaa ataaaatagc gtcgccgggc tatctggctg
    35581 cggacaaaag agacgtgttt ccacctgccc atattttgtt ggaaccatgt aagctctggt
    35641 tgccatttct ggagcgcccg cattccagaa tcggcgctca agcgtgtaat actgtcgttg
    35701 ctcagcaatc gttcccgttg cgggccagga cggtaaatcc ggctgaagag tattcacaac
    35761 ggtcttcatt tcagcagaaa taaggtccag aacaggtagt ttgttttccg gcttcataca
    35821 aaaactcctt tcaaattacg tcattgtaag gaaccactgc catgaaaatg cgatcccgcc
    35881 tgctgatatt gaaactggct gcgtctcgcg cgctcccgtc agattgtgtt aacattcgcc
    35941 gctcagttaa ccacccgtaa aaacaaccat gaaatttccc ggtaaacgta aatccaaaca
    36001 ttacttccct gtaaatgcac gcgatccgct gcttcagcaa ttccagccag aaaacgaaac
    36061 cagcgccgcc tgggtcgtag gtattgacca gacgctggtc gatattgaag cgaaagtgga
    36121 tgatgaattc attgagcgtt atggattaag cgccgggcat tcactggtga ttgaggatga
    36181 cgtagccgaa gcgctttatc aggagctaaa acagaaaaac ctgattaccc atcagtttgc
    36241 gggtggcacc attggtaaca ccatgcacaa ctactcggtg ctcgcggacg accgttcggt
    36301 gctgctgggc gtcatgtgca gcaatattga aattggcagt tatgcctatc gttacctgtg
    36361 taacacttcc agccgtaccg atcttaacta tctacaaggc gtggatggcc cgattggtcg
    36421 ttgctttacg ctgattggcg agtccgggga acgtaccttt gctatcagtc caggccacat
    36481 gaaccagctg cgggctgaaa gcattccgga agatgtgatt gccggagcct cggcactggt
    36541 tctcacctct tatctggtgc gttgcaaacc gggtgaaccc atgccggaag caaccatgaa
    36601 agccattgag tacgcgaaga aatataacgt accggtggtg ctgacgctgg gcaccaagtt
    36661 tgtcattgcc gagaatccgc agtggtggca gcaattcctc aaagatcacg tctctatcct
    36721 tgcgatgaac gaagatgaag ccgaagcgtt gaccggagaa agcgatccgt tgttggcatc
    36781 tgacaaggcg ctggactggg tagatctggt gctgtgcacc gccgggccaa tcggcttgta
    36841 tatggcgggc tttaccgaag acgaagcgaa acgtaaaacc cagcatccgc tgctgccggg
    36901 cgctatagcg gaattcaacc agtatgagtt tagccgcgcc atgcgccaca aggattgcca
    36961 gaatccgctg cgtgtctatt cgcacattgc gccgtacatg ggcgggccgg aaaaaatcat
    37021 gaacactaat ggagcggggg atggcgcatt ggcagcgttg ctgcatgaca ttaccgccaa
    37081 cagctaccat cgtagcaacg taccaaactc cagcaaacat aaattcacct ggttaactta
    37141 ttcatcgtta gcgcaggtgt gtaaatatgc taaccgtgtg agctatcagg tactgaacca
    37201 gcattcacct cgtttaacgc gcggcttgcc ggagcgtgaa gacagcctgg aagagtctta
    37261 ctgggatcgt taagttatcg tcggttcgta ggccagataa ggcgttcacg ccgcatctgg
    37321 catttggctc tcgatgcctg atgcgacgct ggcgcgtctt atcatgccta catatttttc
    37381 atattttaca tccggcaacc accgtttacc ccgtcaccac ttcacccgcc ggtggcgttt
    37441 ccagcagttc cagcatagta cgggcaattt cacgttcacc catcactacc tgattcgcgc
    37501 cacgttcggt gatatacgtc acttcatcgt cataatgggc gcgggcgata atctcaatat
    37561 ccgggttttt cgcgcgggca gacgccacaa tctcacccgc ttcataaccg ttggggatcg
    37621 tcaggatcag ccattttgca cattccagat gcgccagttg cataatttct tcgttcgctg
    37681 cattgcccaa tacagcgcgg accccacgct ctcgcagctc atcaacacgg gttcgtgacg
    37741 tctcaatcac caccagcgga atatcagagg cgagcaattt ctcccccagc aggctgccta
    37801 cacgaccgta acccaccaat agcgcatggt tgcaaatatc cactgggatc tgtttctctt
    37861 cttcgattgc ctcttccagc gtctgctctt ccagcgtttc ggtcttcgcc agatatttct
    37921 ccagtagtgc gaacagtacc gggttgagca taatcgacag gatcgcccct gccagtacca
    37981 ggttttgtcc ggcctgcggc agtaaattca atgccattcc cagtcccgcc aggataaacg
    38041 cgaactcacc aatctgcgcc aggctggcgg cgatggttaa tgccgtacgt tgggagtgac
    38101 caaacagacg gacaaggaac agggccgcga ccgatttacc aaacagaata attgccagcg
    38161 tcgccagcac tgccagcggt tgctgaatca gaattaacgg atcaaacaac atcccgacgg
    38221 agacaaaaaa cagcaccgca aacgcgtcgc gcaatggcag cgtatcgtgg gcggcacggt
    38281 gactcagttc agactcgttc agtaccatcc cggcaaagaa cgcgccaagt gcgaaggaga
    38341 cgtcaaacaa ctccaccgca ccaaaggcaa tccctaacgc cagcgccagc accgacaggg
    38401 taaatagctc gcgagaaccg gttgccgcgc tgcgtgccat aatccacggc accagacggc
    38461 gacctaccag catcataatg gcgataaatg cgatcacttt gccgatggtg atccccatat
    38521 cgactgcaag agtggcaaag cccacatcgc cctgttccat cattcctgcc actgcgggca
    38581 gcaacaccag cgtcagaacc attaccaggt cttccacaat caaccaaccg atggcgattt
    38641 gcccacgctg actgtcaatt aattgccgtt cttcaagtgc gcgcagtaac accacggtac
    38701 tggcggtgga aagacataaa ccgaacacga taccggtcat taacgaccag cccagcacgg
    38761 cagagagcgc catacccagc agcgtcgcca cggctatctg ggcgatcgca ccgggaatgg
    38821 cgatggcctt taccgccatc aaatccttca gcgaaaagtg caaaccgacg ccaaacatca
    38881 acagaatgac gcccagttca gccagttccg gcgcaagctt ggtatcggca acaaagcccg
    38941 gagtgaatgg tcctgccagc acacccgcta acagatatcc caccagagga gaaatacgta
    39001 gtttattggc cagcatgccg aggataaagg cgagcacaag gccgccaaca atggtggtga
    39061 taagcggggt ggcgtgatgc attccgtctc cttttcctgg tggttattgt ccatttttgg
    39121 ccgggaaaac caaaattaca ggtaatagtt tatgacaatt tcattgatga tgttcatgaa
    39181 taattattga attttgcaga aaaatggaat tagctgcaaa aaaagcacgg atcggaaaac
    39241 agaaggcggt ttaacagagg aaaggcttat ggcgactgta aaacgatgca gccaaagttt
    39301 gcctttggct gcaatgaaat cagtctttat gccggttatc aggcaggaat atggtcaaca
    39361 tccccaatag tggcaggaaa gcacagattt tatagactaa ctcgatgct
//
LOCUS       DAEEKQ010000021        39164 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.021, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000021 DAEEKQ010000000
VERSION     DAEEKQ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39164
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.021"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1112)
                     /gene="setA"
                     /locus_tag="KLK57_001069"
     CDS             complement(<1..1112)
                     /gene="setA"
                     /locus_tag="KLK57_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HBE0891504.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAV"
     gene            complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="KLK57_001070"
     CDS             complement(1214..1345)
                     /gene="sgrT"
                     /locus_tag="KLK57_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HBE0891505.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            1434..3089
                     /gene="sgrR"
                     /locus_tag="KLK57_001071"
     CDS             1434..3089
                     /gene="sgrR"
                     /locus_tag="KLK57_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HBE0891506.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      3123..3222
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            3253..4236
                     /gene="thiB"
                     /locus_tag="KLK57_001072"
     CDS             3253..4236
                     /gene="thiB"
                     /locus_tag="KLK57_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HBE0891507.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFTFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQQFLQFMVYPAFQNAIP
                     TGNWMYPVANVTQPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            4212..5822
                     /gene="thiP"
                     /locus_tag="KLK57_001073"
     CDS             4212..5822
                     /gene="thiP"
                     /locus_tag="KLK57_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HBE0891508.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALIQMMCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQSVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDISARYSMLCQSLGIEGWSRL
                     NVVELRALKRPLVQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            5806..6504
                     /gene="thiQ"
                     /locus_tag="KLK57_001074"
     CDS             5806..6504
                     /gene="thiQ"
                     /locus_tag="KLK57_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HBE0891509.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDSVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTDELL
                     SGKASASALLGITG"
     gene            complement(6574..7338)
                     /locus_tag="KLK57_001075"
     CDS             complement(6574..7338)
                     /locus_tag="KLK57_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0891510.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWMAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(7424..8302)
                     /gene="araC"
                     /locus_tag="KLK57_001076"
     CDS             complement(7424..8302)
                     /gene="araC"
                     /locus_tag="KLK57_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HBE0891511.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPPIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            8641..10341
                     /gene="araB"
                     /locus_tag="KLK57_001077"
     CDS             8641..10341
                     /gene="araB"
                     /locus_tag="KLK57_001077"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HBE0891512.1"
                     /translation="MAIAIGLDFGSDSVRALAVECATGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKIVLAELSAEQRAAVVGIGVDTTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATNAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            10352..11854
                     /gene="araA"
                     /locus_tag="KLK57_001078"
     CDS             10352..11854
                     /gene="araA"
                     /locus_tag="KLK57_001078"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HBE0891513.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            12023..12718
                     /gene="araD"
                     /locus_tag="KLK57_001079"
     CDS             12023..12718
                     /gene="araD"
                     /locus_tag="KLK57_001079"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HBE0891514.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            12793..15144
                     /gene="polB"
                     /locus_tag="KLK57_001080"
     CDS             12793..15144
                     /gene="polB"
                     /locus_tag="KLK57_001080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HBE0891515.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGKQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPQLLEKLNAWFANYDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHTSPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            15308..18214
                     /gene="rapA"
                     /locus_tag="KLK57_001081"
     CDS             15308..18214
                     /gene="rapA"
                     /locus_tag="KLK57_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HBE0891516.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPVQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRMEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            18226..18885
                     /gene="rluA"
                     /locus_tag="KLK57_001082"
     CDS             18226..18885
                     /gene="rluA"
                     /locus_tag="KLK57_001082"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HBE0891517.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="KLK57_001083"
     CDS             complement(19002..19817)
                     /gene="djlA"
                     /locus_tag="KLK57_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HBE0891518.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            20072..22426
                     /gene="lptD"
                     /locus_tag="KLK57_001084"
     CDS             20072..22426
                     /gene="lptD"
                     /locus_tag="KLK57_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HBE0891519.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWQHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            22479..23765
                     /gene="surA"
                     /locus_tag="KLK57_001085"
     CDS             22479..23765
                     /gene="surA"
                     /locus_tag="KLK57_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HBE0891520.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            23765..24754
                     /gene="pdxA"
                     /locus_tag="KLK57_001086"
     CDS             23765..24754
                     /gene="pdxA"
                     /locus_tag="KLK57_001086"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HBE0891521.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLNELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            24751..25572
                     /gene="rsmA"
                     /locus_tag="KLK57_001087"
     CDS             24751..25572
                     /gene="rsmA"
                     /locus_tag="KLK57_001087"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HBE0891522.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            25575..25952
                     /gene="apaG"
                     /locus_tag="KLK57_001088"
     CDS             25575..25952
                     /gene="apaG"
                     /locus_tag="KLK57_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HBE0891523.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            25959..26801
                     /gene="apaH"
                     /locus_tag="KLK57_001089"
     CDS             25959..26801
                     /gene="apaH"
                     /locus_tag="KLK57_001089"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HBE0891524.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGTLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(26879..27358)
                     /gene="folA"
                     /locus_tag="KLK57_001090"
     CDS             complement(26879..27358)
                     /gene="folA"
                     /locus_tag="KLK57_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HBE0891525.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="KLK57_001091"
     CDS             complement(27550..29412)
                     /gene="kefC"
                     /locus_tag="KLK57_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HBE0891526.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLHWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGTAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="KLK57_001092"
     CDS             complement(29405..29935)
                     /gene="kefF"
                     /locus_tag="KLK57_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HBE0891527.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGKALHGKYLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(30043..31374)
                     /locus_tag="KLK57_001093"
                     /pseudo
     CDS             complement(30043..31374)
                     /locus_tag="KLK57_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="KLK57_001094"
     CDS             complement(31433..31720)
                     /gene="fixX"
                     /locus_tag="KLK57_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HBE0891528.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQALELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(31717..33003)
                     /locus_tag="KLK57_001095"
     CDS             complement(31717..33003)
                     /locus_tag="KLK57_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE0891529.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFAESAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(33054..33995)
                     /locus_tag="KLK57_001096"
     CDS             complement(33054..33995)
                     /locus_tag="KLK57_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBE0891530.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGAQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(34010..34780)
                     /locus_tag="KLK57_001097"
     CDS             complement(34010..34780)
                     /locus_tag="KLK57_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HBE0891531.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAEAA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            35253..36767
                     /gene="caiT"
                     /locus_tag="KLK57_001098"
     CDS             35253..36767
                     /gene="caiT"
                     /locus_tag="KLK57_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HBE0891532.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVLGLTASTWILWTVLGSNTLLLIDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            36798..37940
                     /gene="caiA"
                     /locus_tag="KLK57_001099"
     CDS             36798..37940
                     /gene="caiA"
                     /locus_tag="KLK57_001099"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HBE0891533.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            38069..39164
                     /gene="caiB"
                     /locus_tag="KLK57_001100"
                     /pseudo
     CDS             38069..39164
                     /gene="caiB"
                     /locus_tag="KLK57_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
BASE COUNT         9363 a        10766 c        10257 g         8778 t
ORIGIN      
        1 acagcaaagt gcccccaact ttgtgcaatt gctccctgaa taacgccagc cagaattacc
       61 ccggtagaaa tactgttagt aaataaggtg gtagctgccc ccgctcttcc aggcattaaa
      121 tcctgaaacc atagcatccc aatacccgca acaatgccga taaatacagc gttaaaaagt
      181 tgcagcgtca tcaacgccat acggctatga aagaaaatca atccggtgta aaacagtact
      241 cctgccgcca ctgctatgac catcattcgc cgcttaccat aacgtttgac atagtagcca
      301 gccagaatca ttgctggtat ttccagtcca gctgccgtcc ccatcaggaa acccgccagt
      361 ttgtctggca atcctaactc gctactgatc cacaacggca tatcaataat gtacatggtg
      421 ttgcaggtcc acattaacgt cgaggcgaca aataacatcc gtacgttact atcctgccag
      481 ccaccttgca ttgataaagc attttccgac ggcagttcta cccgcgccac agacggaagc
      541 ataaatgcaa tcaataccag actgagtgtg aatatcccgg cggcaatcga aaacatcacc
      601 gtaaagccgt aattcaacgc cagcataaag gccaacggtg gaccgataac ccatgccaga
      661 gaaagctgcg cacgcatcac cgagctaaac atcaccactt ctcgcgccga gttatccgca
      721 tattcccgcg ccagagcaaa taactgtggc attgccgtat tggccagaga tgccagaagc
      781 acaccacagg tgataagcgt cagataatga cgattaaatg caaacaatag cgcattgccg
      841 atagccatca aacagcaaaa tataatcagt tttcgccgat cgccctgact gtcagaacgt
      901 tttgccaacc agaggcttac gccgatccca gcaatagcat tcaccgtata aaagagtccg
      961 atccagaaag gttgcgcgcc aacctcacga ctcagaaata agctcaatgt aggagcctgt
     1021 agcgccccgg ccacccccat cataaaagcg accagcataa atgccgcgta aacaccgttc
     1081 atacggcgag ccatcgtcat tatccagatc atacgttccc tttttagcgc ggcgagaata
     1141 aaaaaaacca gcaggtataa tctgctggcg ggtgatttta caccaatact cagtcacaca
     1201 tgatgcaggc aagtcaactt tcagaattgc ggtcatccca ctgcatcagt ccttcaagca
     1261 tggttaatcg ttgtgggacg cttaaccaac gcaaccagca caacttcgct gtcgcggtaa
     1321 aatagtgctg ataaaactga cgcatggggc acctccttgc ttcatcgtta ggaattattg
     1381 acttaatata gggaaaataa aattgctgtc ttttgcgcag gagttcccct tttatgccat
     1441 ctgctcgtct gcaacaacag ttcatccgcc tgtggcaatg ctgcgagggt aaatcgcagg
     1501 acacaacgct caacgaactg gcagcgttat tgagctgctc gcgtcgtcat atgcgtaccc
     1561 tgctcaacac catgcaggat cgcggctggc tgacgtggga agcggaagtc gggcgcggta
     1621 aacgctcgcg tctgacattc ctctataccg ggctggcgct tcagcaacag cgggcggaag
     1681 acctgctgga gcaggatcgt atcgaccaac tagtgcagtt ggttggcgac aaagcgactg
     1741 tgcggcaaat gctggtttct catctgggcc gcagcttccg ccaggggcgg cacatcctgc
     1801 gcgtgctcta ctatcgtccg ttgcgtaatc tgctacctgg cagcgcattg cgccgttccg
     1861 aaacccatat tgcccggcaa atcttcagtt ccctaacgcg cataaatgag gaaaatgggg
     1921 aactggaagc agacatcgcc caccactggc agcaaatatc accgcttcac tggcgtttct
     1981 ttttgcgtcc aggagtccat tttcatcatg gtcgtgaact ggaaatggac gacgtgatcg
     2041 cctctttaaa acgaatcaat acgctgccgc tctattcgca tattgcggac attgtgtcgc
     2101 cgacgccctg gacgctggat atccatctca cgcaaccgga ccgctggtta ccgttactgc
     2161 tggggcaagt tccggcgatg atcctgccgc gcgaatggga aaccctcagt aactttgcca
     2221 gccatcccat cggcaccggt ccgtatgcgg tgatccgcaa caccaccaat caactgaaaa
     2281 ttcaggcatt cgatgacttc ttcggttacc gggcattaat cgatgaagtt aacgtctggg
     2341 ttctgccgga aattgccgac gagccagctg gaggtctgat gctaaaaggt ccacagggtg
     2401 aggaaaaaga gattgaaagc cgcctggagg aaggttgcta ctatttacta ttcgacagcc
     2461 gcacccatcg cggggcgaat cagcaagtca gggactgggt aagctatgtg ctttctccaa
     2521 ctaatctggt ctatttcgct gaggaacagt accagcaact gtggttcccg gcttatggac
     2581 tgctcccccg ttggcaccat gcccgcacca taaagagcga aaaaccggct ggcctggaaa
     2641 gcctcaccct gaccttttat caggatcaca gtgagcatcg ggtgattgcc gggatcatgc
     2701 agcagattct ggcaagtcac caggtcacgc tggaaatcaa agagatcagc tacgatcagt
     2761 ggcatgaagg agagatcgag agcgatatct ggcttaacag cgccaacttt acgctgccgc
     2821 tggatttttc gctgttcgcg cacttgtgcg aggtgccgct gctccaacac tgtattccga
     2881 tcgactggca agccgacgcc gcccgctggc gcaatggcga aatgaacctg gcgaactggt
     2941 gccagcaact ggtcgccagc aaagcaatgg tgccgcttat ccaccactgg ctgatcattc
     3001 aggggcaacg cagtatgcgc ggcctgcgca tgaacaccct cggctggttc gattttaaat
     3061 cagcgtggtt tgcgccaccg gatccatgat tgctggtaga taacaaaatc actacactaa
     3121 cgccgttctc aacggggtgc cacgcaaacg cgtgcgctga gaaaataccc gtcgaacctg
     3181 atccggataa cgccgacgaa gggatttgag gctccttctc aagtcctttg ccactctttt
     3241 ttgaggtgca aagtgttaaa aaaatgtctt cccctgctgt tgctgtgcac agcgcccgtt
     3301 ttcgctaaac ccgttctgac tgtttatacc tacgattcct tcgccgccga ctgggggcct
     3361 ggtccaaaga tcaaaaaagc ttttgaagcc gactgtaact gcgaactgaa actggtggcg
     3421 ctggaagatg gcgtttcgct tctcaaccgt ctacggatgg aaggcaaaaa tagtaaagcc
     3481 gatgtggtgc tggggctgga taacaatctg ttagacgccg ccagtaaaac cggactattt
     3541 gccaaaagcg gtgtggcagc ggatgccgtt aacgttcccg gcggctggaa taatgacact
     3601 ttcgtcccgt ttgattacgg ctatttcacc ttcgtctatg acaagaacaa actgaaaaac
     3661 ccgccgcaaa gcctgaaaga attggttgag agcgatcaga actggcgggt gatttatcag
     3721 gatccgcgta ccagcacgcc ggggctgggt ctgctgttgt ggatgcaaaa agtctatggc
     3781 gatgacgccc cacaagcctg gcagaaactg gcgaagaaaa ctgtcacggt caccaaaggc
     3841 tggagcgaag cctatggcct gtttttaaaa ggcgaaagcg atctggtact gagttacacc
     3901 acctctccgg cttatcacat tctcgaagag aagaaagata actacgccgc cgcgaacttc
     3961 agcgaaggtc actatctgca ggtggaagtc gccgcccgca ccgctgccag caagcagccg
     4021 gagctggcgc aacaattcct ccagtttatg gtttatccgg cttttcagaa tgcgatccca
     4081 accggcaact ggatgtatcc ggtggcaaac gtcacgcagc ctgccggttt tgaacaattg
     4141 accaaaccag caaccacgct ggagttcacg ccagccgaag tggcggcaca acgtcaggca
     4201 tggattagcg aatggcaacg cgccgtcagc cgttaattcc cggctggtta attccaggtg
     4261 taagcgccgc cacgctggtg gtagcggttg cgctcgcggc gtttctcgcc ctgtggtgga
     4321 acgcgccgca gggtaactgg gtagcagtct ggcaggacag ctacctgtgg catgtggtgc
     4381 gcttctcctt ctggcaggcg tttctctcgg cgctactctc tgtcgtaccc gcgatattcc
     4441 tcgcccgcgc cctctatcgc aggcgctttc cgggtcggct ggcgctgttg cgtctgtgcg
     4501 caatgacctt gatcctcccg gtgctggttg ctgttttcgg cattcttagc gtctatggtc
     4561 gtcagggctg gctggcatcg ctctgccaat cgctcggtct ggagtggacc ttttcgccct
     4621 acggcctgca aggcattttg ctggcccatg tgttttttaa tctgccgatg gcgagccgtt
     4681 tattacttca ggcactggaa aacattcccg gcgaacaacg tcaacttgcc gcccagcttg
     4741 gaatgcgtgg ctggcacttc tttcgtttcg tcgaatggcc gtggttacgg cgacaaatcc
     4801 cgccagttgc tgcgcttatc tttatgctct gtttcgccag cttcgccacc gtactgtcgc
     4861 taggcggcgg tccgcaagcg accactatcg agctggctat ttatcaggcg ctgagttacg
     4921 actacgatcc tgcccgcgcg gcgatgctgg cgctgatcca gatgatgtgt tgccttgggc
     4981 tggtgctgtt gagtcagcga ttgagtaagg ccattgcgcc cggcaccacg ctgctgcaag
     5041 gctggcgcga cccggacgat cgtctgcata gccgcatttg cgacacggtg ttaatagtgc
     5101 tggcgctgct gctgttgttg ccaccgttgc tggcggtgat cgtcgatggg gtaaatcgcc
     5161 agttgccgga agtgctggca caatcggtgc tgtggcaggc gctgtggacc tcgttgcgta
     5221 ttgcgctggc ggcaggtgta ttgtgcgtag tgctgaccat gatgctgcta tggagcagtc
     5281 gcgaacttcg ggcgcggcag aaaatgctgg cgggtcaggc gctggagatg agcggcatgt
     5341 tgatcctcgc catgccgggg attgtgctgg ctaccggctt ctttttactg ctcaacaaca
     5401 ctatcggcct gccacaatct gctgacggca ttgtgatttt caccaatgcg ttaatggcga
     5461 tcccttacgc gctgaaagta ctggaaaacc cgatgcgcga tatctccgcc cgctacagca
     5521 tgttgtgtca gtcgttgggc attgaaggct ggtcacgctt aaacgtggtg gagctgcgcg
     5581 ccctgaaacg cccactggtg caggcgctgg cctttgcatg cgtgctgtcg attggtgatt
     5641 ttggcgtggt ggcgttgttc ggtaacgatg atttccgcac cctgccgttt tatctctacc
     5701 agcaaattgg ctcctatcgc agccaggacg gcgcggtcac cgcgttaatt ctgctactac
     5761 tctgttttct gctgtttacc gtgattgaaa aactaccggg gcgaaatgtt aaaactgact
     5821 gatatcacct ggctttacca ccatttgccg atgcgtttta gcttaacggt agaacgcggc
     5881 gagcaggtgg cgatcctcgg gccaagcggc gcaggtaaaa gtaccctgct gaatttgatt
     5941 gccggttttc tgacgccagc cagcggttcg ctgactatcg acagcgttga tcacaccacg
     6001 acgccgccgt cacgccgtcc ggtatcgatg ctgtttcagg agaacaacct gttcagccac
     6061 ctgacggtcg cgcagaacat cgggctgggg cttaatccgg gattgaagct gaacgcggcg
     6121 cagcaggaga aaatgcacgc tatcgcccgc cagatgggga ttgataattt aatggcgcgg
     6181 ttaccgggcg agctttccgg cggtcagcga cagcgcgtgg cgttagcgcg ttgtctggta
     6241 cgcgagcagc cgattttatt gctcgatgaa ccgttctctg cgctcgatcc ggcgctacgc
     6301 caagagatgt tgacgctggt gagcacgagc tgccagcagc aaaaaatgac gttattgatg
     6361 gtgtcgcaca gtgtggaaga tgccgcgcgg atcgccacgc gctcggtggt ggtcgccgac
     6421 gggcgcatcg cctggcaggg taaaaccgat gaattgttaa gcggtaaggc gagtgcttcg
     6481 gcgctgttgg gaattacagg ttaaaagcgc acggacagtc ccctcgcccc tctggggaga
     6541 agggaacaaa ccagcgccga tgcaaactat cccctaaacc ccaaccactt tacgcaaaat
     6601 atcgatatac accggcatca acgggtggcg aattaacacc accagcgcca ccacgccgat
     6661 ggcagaaatc aacggcgtca gccacaacaa acgaccgcgg gacaaataat gactcaaacg
     6721 gtcagtcgct ttaccactgc gccataaccg ccagcacagc cagccaccaa cccacaaaaa
     6781 taccgccgtc gccagcagca accatttaaa ctcaccgctc tgcatcccgg caggaatatc
     6841 gatcgccgcg cccgccagaa tccctggcag gaagtaaaac ggcggccaca gcaggcagcc
     6901 gataatattc ggcgtgataa attttgccac tggcagatcc agcattcccg ccaccattgg
     6961 caccagcgga cgcgtcgggc caacaaaacg accgaccaga atggtgaaca tgctgtgttg
     7021 atgcaacgca tgttcagttt tatcaagtag tgctttgttt ttcttcagaa atgaccagcg
     7081 atgcaacggc tttttaaaac gccagcccag ccagaaggag atccagtcgc ccagcaggca
     7141 accgacaatc cccgccatcc acgcgtgcca gaaacttaac tcgccgctgc caatcagcgc
     7201 tcccagcccc gccatcagca ccgtaccggg tagaatcaaa ccgaccagcg ccagcgactc
     7261 cagaaaggcc accaacacca ccgccatcaa tgaatacacg gtggattggg taataaagtg
     7321 ttccagcaat gcttgcataa tgtgcctgtc aaatggacga agcagggatt ctgcaaaccc
     7381 tatgctactc cgtcaagccg tcaattgtct gattcgttac caattatgac aacttgacgg
     7441 ctacatcatt cactttttct tcacaaccgg cacggaactc gctcgggctg gccccggtgc
     7501 attttttaaa tacccgcgag aaatagagtt gatcgtcaaa accaacattg cgaccgacgg
     7561 tggcgatagg catccgggta gtgctcaaaa gcagcttcgc ctgactaatg cgttggtcct
     7621 cgcgccagct taagacgcta atccctaact gctggcggaa aagatgtgac agacgcgacg
     7681 gcgacaagca aacatgctgt gcgacgctgg cgatatcaaa attgctgtct gccaggtgat
     7741 cgctgatgta ctgacaagcc tcgcgtacac gattatccat cggtggatgg agcgactcgt
     7801 taatcgcttc catgcgccgc agtaacaatt gctcaagcag atttatcgcc agcagctccg
     7861 aatagcgccc ttccccttgc ccggcgttaa tgatttgccc aaacaggtcg ctgaaatgcg
     7921 gctggtgcgc ttcatctggg cgaaagaaac ccgtattggc aaatattggc ggccagttaa
     7981 gccattcatg ccagtaggcg cgcggacgaa agtaaaccca ctggtgatac cattcgcgag
     8041 cctccggatg acgaccgtag tgatgaatct ctcctggcgg gaacagcaaa atatcacccg
     8101 gtcggcagac aaattctcgc ccctgatttt tcaccacccc ctgaccgcga atggtgagat
     8161 tgagaatata acctttcatt cccagcggtc ggtcgataaa aaaatcgaga taaccgttgg
     8221 cctcaatcgg cgttaaaccc gccaccagat gggcgttaaa cgagtatccc ggcagcaggg
     8281 gatcattttg cgcttcagcc atacttttca tactcccgcc attcagagaa gaaaccaatt
     8341 gtccatattg catcagacat tgccgtcact gcgtctttta ctggctcttc tcgctaaccc
     8401 aaccggtaac cccgcttatt aaaagcattc tgtaacaaag cgggaccaaa gccatgacaa
     8461 aaacgcgtaa caaaagtgtc tataatcacg gcagaaaagt ccacattgat tatttgcacg
     8521 gcgtcacact ttgctatgcc atagcatttt tatccataag attagcggat cctacctgac
     8581 gctttttttc gcaactctct actgtttctc catacccgtt tttttggatg gagtgaaacg
     8641 atggcgattg caattggcct cgattttggc agtgattctg tgcgagcttt ggcggtggaa
     8701 tgcgctaccg gtgaagagat cgccaccagc gtagagtggt atccccgttg gcagaaaggg
     8761 caattttgtg atgccccgaa taaccagttc cgccatcatc cgcgtgacta cattgagtca
     8821 atggaagcgg cgctgaaaat cgtgcttgca gagcttagcg ccgaacagcg cgcagctgtg
     8881 gtcgggattg gcgttgacac aaccggctcg acgcccgcac cgattgacgc cgacggtaac
     8941 gtgctggcgc tgcgcccaga gtttgccgaa aacccaaacg cgatgttcgt attgtggaaa
     9001 gaccacactg cggttgaaga agcggaagag attacccgtt tgtgccacgc gccgggcaac
     9061 gttgactact cccgctatat tggcggtatt tattccagcg aatggttctg ggcaaaaatc
     9121 ctgcatgtga ctcgccagga cagcgccgtg gcgcaatctg ccgcatcgtg gattgagctg
     9181 tgcgactggg tgccagctct gctttccggt accacccgcc cgcaggatat tcgtcgcgga
     9241 cgttgcagcg ccgggcataa atctctgtgg cacgaaagct ggggcggctt gccgccagcc
     9301 agtttctttg atgagctgga cccgatcctc aatcgccatt tgccttcccc gctgttcact
     9361 gacacctgga ctgccgatat tccggtgggc accttatgcc cggaatgggc gcagcgtctc
     9421 ggcctgcctg aaagcgtggt gatttccggc ggcgcgtttg actgccatat gggcgcagtt
     9481 ggcgcaggcg cacagcctaa cgcactggta aaagttatcg gtacttccac ctgcgacatt
     9541 ctgattgccg acaaacagag cgttggcgag cgggcagtta aaggtatttg cggtcaggtt
     9601 gatggcagcg tggtgcctgg atttatcggt ctggaagcag gccaatcggc gtttggtgat
     9661 atctacgcct ggtttggtcg cgtactcggc tggccgctgg aacagcttgc cgcccagcat
     9721 ccggaactga aagcgcaaat caacgccagc cagaaacaac tgcttccggc gctgaccgaa
     9781 gcatgggcca aaaatccgtc tctggatcac ctgccggtgg tgctcgactg gtttaacggt
     9841 cgtcgcacgc caaacgctaa ccaacgtctg aaaggggtga ttaccgatct taacctcgct
     9901 accaacgctc cgctgctgtt cggcggtttg attgctgcca ccgcctttgg cgcacgcgca
     9961 atcatggagt gctttaccga tcaggggatc gccgtcaata acgtgatggc gctgggcggc
    10021 atcgcgcgga aaaaccaggt cattatgcag gcctgctgcg acgtgctgaa tcgcccgctg
    10081 caaattgttg cctctgacca gtgctgtgcg ctcggtgcgg cgatttttgc tgccgtcgcc
    10141 gcgaaagtgc acgcagacat cccatcagcc cagcaaaaaa tggccagtgc ggtagagaaa
    10201 accctgcaac cgcgcagcga acaggcacaa cgctttgaac agctttatcg ccgctatcag
    10261 caatgggcga tgagcgccga acaacactat cttccaactt ccgccccggc acaggctgcc
    10321 caggccgttg cgactctata aggacacgat aatgacgatt tttgataatt atgaagtgtg
    10381 gtttgtcatt ggcagccagc atctgtatgg cccggaaacc ctgcgtcagg tcacccaaca
    10441 tgccgagcac gttgttaatg cgctgaatac ggaagcgaaa ctgccctgca aactggtgtt
    10501 gaaaccgctg ggcaccacgc cggatgaaat caccgctatt tgccgcgacg cgaattacga
    10561 cgatcgttgc gctggtctgg tggtgtggct gcacaccttc tccccggcca aaatgtggat
    10621 caacggcctg accatgctca acaaaccgtt gctgcaattc cacacccagt ttaacgcggc
    10681 gctgccgtgg gacagtatcg atatggactt tatgaacctg aaccagactg cacatggcgg
    10741 tcgcgagttc ggcttcattg gcgcgcgtat gcgtcagcaa catgccgtgg ttaccggtca
    10801 ctggcaggat aaacaagccc atgagcgtat cggctcctgg atgcgccagg cggtctctaa
    10861 acaggatacc cgtcatctga aagtctgccg ttttggcgat aacatgcgtg aagtagcggt
    10921 caccgatggt gataaagttg ccgcacagat caagttcggt ttctccgtca atacctgggc
    10981 ggttggcgat ctggtgcagg tggtgaactc catcagcgac ggcgatgtta acgcgctggt
    11041 cgatgagtac gaaagctgct acaccatgac gcctgccaca caaatccacg gcgaaaaacg
    11101 acagaacgtg ctggaagcgg cgcgtattga gctggggatg aagcgtttcc tggaacaagg
    11161 tggcttccac gcgttcacca ccacctttga agatttgcac ggtctgaaac agcttccagg
    11221 tctggccgta cagcgtctga tgcagcaggg ttacggcttt gcgggcgaag gcgactggaa
    11281 aaccgccgcc ctgcttcgca tcatgaaggt gatgtcaacc ggtctgcagg gcggcacctc
    11341 ctttatggag gactacacct atcacttcga gaaaggtaat gacctggtgc tcggctccca
    11401 tatgctggaa gtctgcccgt cgattgccgt agaagagaaa ccgatcctcg acgttcagca
    11461 tctcggtatt ggtggtaagg acgatcctgc ccgactgatc ttcaataccc aaaccggtcc
    11521 agcgattgtc gccagcctga ttgatctcgg cgatcgttac cgtctgctgg ttaactgtat
    11581 cgacacggtg aaaacaccgc actccctgcc gaaactgccg gtggcgaatg cgctgtggaa
    11641 agcgcaaccg gatctgccaa ctgcttccga agcgtggatc ctcgctggtg gcgcgcacca
    11701 taccgtcttc agccatgcgc tgaacctcaa cgatatgcgc cagttcgccg agatgcacga
    11761 cattgaaatc acggtgattg ataacgacac ccgcctgcca gcgtttaaag acgcactgcg
    11821 ctggaacgaa gtgtattacg gatttcgtcg ctaagtagcc acatcaggta tgtaatgcct
    11881 gatgcgacgc tgacgcgtct tatctggcct acacgctgcg attttgtagg ccggataagc
    11941 aaagcgcatc cggcattgtt ttccccctcg cccctttggg gagagggccg gggtgagggg
    12001 aaacgacctc tcaggagtca acatgttaga agatctcaaa cgccaggtat tagaagccaa
    12061 cctggcgctg ccaaaacaca acctggtcac gctcacatgg ggcaacgtca gcgccgttga
    12121 tcgcgagcgc ggcgtctttg tgatcaaacc ttccggcgtc gattacagcg tcatgaccgc
    12181 tgacgatatg gtcgtggtta gcatcgaaac cggtgaagtg gttgaaggta cgaaaaagcc
    12241 ctcctccgac acgccaactc accggctgct ctatcaggca ttcccctcca ttggcggcat
    12301 tgtgcatacg cactcgcgcc acgccaccat ctgggcgcag tcgggtcagt cgattccagc
    12361 aaccggcacc acccacgccg actatttcta cggcaccatt ccctgcaccc gcaaaatgac
    12421 cgacgcagaa atcaacggcg aatatgagtg ggaaaccggt aacgtcatcg tagaaacctt
    12481 tgaaaaacag ggtatcgatg cagcgcaaat gcccggcgtt ctggtccatt cccacggccc
    12541 gtttgcatgg ggcaaaaatg ccgaagatgc ggtgcataac gccatcgtgc tggaagaggt
    12601 cgcttatatg gggatattct gccgtcagtt agcgccgcag ttaccggata tgcagcaaac
    12661 gctgctggat aaacactatc tgcgtaagca tggcgcgaag gcatattacg ggcagtaatg
    12721 actgtataaa accacagcca atcaaacgaa accaggctat actcaagcct ggttttttga
    12781 tggattttca gcgtggcgca ggcaggtttt atcttaaccc gacactggcg ggacaccccg
    12841 caagggacag aagtctcctt ctggctggcg acggacaacg ggccgttgca ggttacgctt
    12901 gcaccgcaag agtccgtggc gtttattccc gccgatcagg ttccccgcgc tcagcatatt
    12961 ttgcagggta aacaaggctt tcgcctgaca ccgctggcgt taaaggattt tcaccgccag
    13021 ccggtgtatg gcctttactg tcgcgcccat cgccaattga tgaattacga aaagcgcctg
    13081 cgtgaaggtg gcgttaccgt ctacgaggcc gatgtgcgcc cgccagaacg ctatctgatg
    13141 gagcggttta tcacctcacc ggtgtgggtc gagggtgata tgcacaatgg cactatcgtt
    13201 aatgcccgtc tgaaaccgca tcccgactat cgtccgccgc tcaagtgggt ttctatagat
    13261 attgaaacca cccgccacgg tgagctgtac tgcatcggcc tggaaggctg cgggcagcgc
    13321 atcgtttata tgctggggcc ggagaatggc gacgcctcct cgcttgattt cgaactggaa
    13381 tacgtcgcca gccgcccgca gttgctggaa aaactcaacg cctggtttgc caactacgat
    13441 cctgatgtga tcatcggttg gaacgtggtg cagttcgatc tgcgaatgct gcaaaaacat
    13501 gccgagcgtt accgtcttcc gctgcgtctt gggcgcgata atagcgagct ggagtggcgc
    13561 gagcacggct ttaaaaacgg cgtctttttt gcccaggcta aaggtcggct aattatcgac
    13621 ggtatcgagg cgctgaaatc cgcgttctgg aatttctctt cattctcgct ggaaactgtc
    13681 gctcaggagc tattaggcga aggaaaatct atcgataacc cgtgggatcg aatggacgaa
    13741 attgaccgcc gtttcgccga agataaacct gcgctggcaa cttataacct gaaagattgc
    13801 gagctggtga cgcagatctt ccacaaaact gaaatcatgc catttttact cgaacgagca
    13861 acggtgaacg gcctgccggt ggatcggcac ggcggttcgg tggcagcgtt tggtcatctc
    13921 tattttccgc gaatgcatcg cgctggttat gtcgcgccta atctcggcga agtgccgccg
    13981 cacaccagcc ctggcggcta cgtgatggat tcacggccag ggctttatga ttcagtgctg
    14041 gtgctggact ataaaagcct gtacccgtcg atcatccgca cctttctgat tgatcccgtc
    14101 gggctggttg aaggcatggc gcagcctgat ccagagcaca gtaccgaagg ttttctcgat
    14161 gcctggttct cacgggagaa acattgcctg ccggagattg tgactaacat ctggcacggg
    14221 cgcgatgaag ccaaacgtca gggtaataaa ccactttcgc aggcgctgaa gatcattatg
    14281 aatgcctttt atggcgtgct tggcaccacc gcctgccgct ttttcgatcc gcgcctggca
    14341 tcgtcgatca ccatgcgtgg tcatcagatc atgcggcaaa ccaaagcatt gattgaagcg
    14401 cagggctacg acgttatcta cggagatacc gactcgactt ttgtctggct aaaaggtgca
    14461 cattcggaag aagaggcggc gaaaatcggt cgtgcactgg tgcagcacgt taacgcctgg
    14521 tgggcggaaa cgctgcaaaa acaacggctg accagcgcat tagaactgga gtatgaaact
    14581 catttctgcc gttttcttat gccaaccatt cgcggagctg ataccggcag taaaaagcgc
    14641 tacgccgggc tgattcagga gggcgataaa cagcggatgg tgtttaaagg gctggaaacc
    14701 gtgcgcaccg actggacgcc gctggcccag cagtttcagc aggagctata cctgcgcatc
    14761 ttccgcaacg agccatatca ggaatatgta cgcgaaacca tcgacaaact gatggcgggt
    14821 gaactggatg cgcgactggt ttaccgtaaa cgccttcgcc gtccgctgag cgagtatcag
    14881 cgtaatgtgc cgcctcatgt acgcgccgct cgccttgccg atgaagaaaa ccaaaagcgt
    14941 ggtcgcccct tgcaatatca gaatcgcggc accattaagt acgtatggac caccaacggc
    15001 ccggagccgc tggactacca acgttcacca ctggattacg aacactatct gacccgccag
    15061 ctacaacccg tggcggaggg aatactccct tttattgagg ataattttgc tacacttatg
    15121 accgggcaac ttgggctatt ttgagcaaaa aaagagttcg ccagatacca ttttgatgcg
    15181 tgacgaatgc tttgccatcc agtaccatag cgccctttcc attcctggac ctgaataaca
    15241 ccactacccc ataagcacgg tagcgggtgg ttattgcctg caattaaaga tatagagccg
    15301 aacacatatg ccttttacac ttggtcaacg ctggatcagc gatacagaaa gcgaattggg
    15361 acttggaacc gttgtcgcgg tggatgcgcg aactgtcact ttacttttcc catctactgg
    15421 tgaaaaccgt ctgtacgcac gcagtgattc ccccgtgacc cgcgtgatgt tcaaccctgg
    15481 tgatactatt accagccatg acggctggca gatgcaagtc gaagaagtaa aagaagaaaa
    15541 tggcttgctg acctatatcg gtactcgcct ggatactgaa gagtccggcg tagccctgcg
    15601 tgaagttttc cttgatagca aactggtgtt cagcaaaccg caggaccgtc tgtttgccgg
    15661 gcagattgac cgtatggacc gctttgcgct gcgttatcgc gcgcgtaagt attccagcga
    15721 acagttccgt atgccgtaca gcggcctgcg cggtcagcgt accagcctga tcccgcatca
    15781 gctcaacatc gctcatgatg ttggtcgccg ccacgcgccg cgcgtcctgc tggctgacga
    15841 agtgggttta gggaaaacca ttgaagccgg gatgatcctg catcagcaac tgctctctgg
    15901 cgctgctgaa cgtgtgctaa ttatcgtccc ggaaacctta cagcatcagt ggctggtaga
    15961 aatgctgcgc cgtttcaacc tgcgctttgc gctatttgat gatgagcgtt atgccgaagc
    16021 tcagcacgat gcttacaacc cgttcgacac cgaacagctg gtgatttgct cgctggattt
    16081 tgcccgtcgt agcaaacagc gcctggaaca tctctgtgaa gccgaatggg acctgctggt
    16141 ggtcgatgaa gcgcatcacc tggtgtggag cgaagatgcg ccaagccgtg aatatcaggc
    16201 cattgaacaa ctggcagagc acgtgccggg cgttctgctg ctgaccgcga ccccggaaca
    16261 gctggggatg gaaagccact tcgcccgtct gcgtctgctg gacccgaacc gtttccacga
    16321 ttttgcgcag ttcgttgaag agcagaaaaa ttatcgtccg gttgcggacg ccgttgccat
    16381 gctgctggca ggtaacaaac tgagcaatga cgaactgaac atgctcggcg agatgatcgg
    16441 cgagcaggat atcgagccgc tgttgcaggc agcaaacagc gacagcgaag atgcccagag
    16501 cgcccgtcag gagctggttt cgatgctgat ggatcgccac ggcaccagcc gcgtgctgtt
    16561 ccgtaacacg cgtaacggtg tgaaaggatt cccgaaacgc gagctgcaca ccattaagct
    16621 gccgctgccg acgcagtatc agacggctat taaagtctcc ggcattatgg gcgcacgtaa
    16681 aagtgcggaa gatcgtgctc gcgatatgct ctacccggag cgtatttatc aggaatttga
    16741 aggtgataac gccacctggt ggaacttcga tccgcgcgtt gagtggctga tgggctacct
    16801 gaccagccat cgctctcaga aagtgctggt gatctgcgcc aaagctgcca ctgcgctgca
    16861 actggagcag gtactgcgcg aacgtgaagg tattcgcgct gcggtgttcc acgaaggtat
    16921 gtcgattatc gaacgtgacc gcgctgccgc ctggtttgcc gaagaagaca ccggcgcaca
    16981 ggtactgctg tgctcagaaa tcggttctga aggacgtaac ttccagttcg ccagccacat
    17041 ggtgatgttt gacctgccat tcaacccgga tctactggag cagcgtattg gtcgtctgga
    17101 tcgtatcggc caggcgcacg atattcagat ccatgtgcct tatctggaga aaaccgctca
    17161 gtcggtgctg gtgcgctggt atcacgaagg tctggatgca tttgagcaca cctgcccgac
    17221 cggacgcact atttacgata gcgtatacaa cgatctgatt aactatctgg cttcaccggt
    17281 tcaaaccgaa ggctttgacg atctgatcaa aaactgccgc gagcaacatg aagcgctgaa
    17341 agcacagctg gaacagggtc gtgaccgcct gctggaaatc cactccaacg gtggcgaaaa
    17401 agcccaggca ctggcagaaa gcattgaaga gcaggatgac gataccaacc tgatcgcctt
    17461 cgccatgaac ctgttcgata ttatcggtat caatcaggac gatcgcggcg acaacatgat
    17521 cgtgctgacg ccgtccgatc atatgctggt gccggacttc cctggcctgt cggaagatgg
    17581 catcaccatc acctttgatc gtgaagtggc gctggcgcgt gaagatgcac agtttattac
    17641 ctgggagcat ccgctgatcc gcaacggtct ggatctgatc ctttctggcg atactggtag
    17701 cagcacgatt tcactattga aaaacaaagc gttgccggta ggtacgctgt tggtggaact
    17761 gatttatgtc gttgaagccc aggctccgaa gcagttgcag ctcaaccgct tcctgccacc
    17821 gacgccggta cgtatgctgc tggataaaaa cggcaacaac ctggcggcgc aggtagagtt
    17881 tgaaaccttt aaccgtcagc ttaacgcggt taaccgtcac accggcagca aactggttaa
    17941 cgccgtgcag caggatgttc acgctatcct tcagctgggt gaagcgcaga tcgagaaatc
    18001 tgcccgtgca ttgattgatg ccgcacgtaa cgaagccgac gaaaaactgt ctgccgagct
    18061 gtctcgtatg gaagcgctgc gcgcagtgaa cccgaacatt cgtgacgacg aactgaccgc
    18121 cattgagagc aaccgtcagc aggtaatgga aagcctggat caggcaggct ggcgtctgga
    18181 tgccctgcgt ttgatcgttg taacgcatca gtaacggagc cgaaaatggg gatggaaaac
    18241 tacaatccac cgcaggaacc ctggttggtt atcctgtatc aggatgacca cattatggtg
    18301 gttaacaagc cgagcggttt gttgtctgtg ccggggcgtc tggaagagca caaagacagc
    18361 gtgatgacgc gcattcagcg tgattatccg caggcagaat cggtgcatcg tctggatatg
    18421 gctaccagcg gcgtgattgt agtggcgctg accaaagccg cggagcggga gttaaaacgc
    18481 cagttccgcg agcgcgagcc gaaaaagcag tatgtagccc gcgtctgggg gcatccatcc
    18541 cctgcggaag gtctggtgga tctgccgctg atttgcgact ggccaaaccg cccgaaacag
    18601 aaagtctgtt acgaaacggg taaacctgcg cagacggaat atgaagtggt ggagtatgcg
    18661 gcggataaca cggcaagagt ggtgttaaaa ccgattaccg ggcgttcgca tcagctgcgt
    18721 gtgcatatgc tggcgctggg tcatccgatt ctcggcgatc gtttttatgc atcaccagaa
    18781 gcgagagcga tggcaccgcg tttgttgcta catgcagaga tgttgacgat tacccatccg
    18841 gcgtatggca atagtatgac gtttaaagcg ccagcggatt tttaatcgtc acgctggcgc
    18901 agttcggtag gcggataagg cgttcacgcc gcatccggca attgtgcgcc gattgcctga
    18961 tgcgacgcgg gccgcgtctt atcaggccta cagtttaaaa cttatttaaa ccctttctgc
    19021 tgctttatca gctcatatgc ctgctgaatt tcctgcgctt tctgcttcgc catctccatc
    19081 atctcaggcg gcaaaccttt cgccaccagc ttatcgggat ggtgttcact catcagctta
    19141 cggtaggcac gtttgatggt ggtcgcatca tccgtcggct tcacgcccag cacattacag
    19201 gcatcttcca gcgtcgggcc acgctgcgct tgctgccagt taccaccgcc agattgctgc
    19261 tgataaccgc cgccaaactg tgcaccgccc tgcatcatgc gcaaaaactg gtcaaactgg
    19321 gcgcgggaga tccctaattc ttcagcaatg acatacagca ccgcccgttc attcgggtgc
    19381 agtgaaccat cagcaaacgc cgcctgaatc tggatttcca gaaacatacg aattaagtca
    19441 aaacgaccaa agcagacact gcgaaactgg cgcatctttt cgcgcagcgg gtaattatct
    19501 gattttccca cccggaacgc gttttgcgcc gcagtacggg aagcgccatg aaggttcatt
    19561 cggtccatca actggctggc gatatgaata tcagcctccg tgacgcgacc tttggatttg
    19621 gttaaatgcc ccatcacttc aaaagtggtg gcaaaaaaca gcgcctgacg ctcacgctgg
    19681 ttggcgaacc acgccatttt acggctacgg gctttatcaa acatatggcc aattaacagg
    19741 cctaacacta cgccccaaaa gccgccgccc atcagtaagg ccacggccac gccaatgatt
    19801 tttccccaat actgcatata ttccccaaat cgacacacgg atatcagggc tatctcccac
    19861 aatataaagg tgcttttacc gttttccggc tgcggtcaat tgtgggacat cgcctataat
    19921 ttgcattatc atacctgtca ttcaatgtcg tgcctaacac cactgacgct attcggacag
    19981 gattaacact agcgtagaga tgacgagtac gttagtctct gaccgtttgt cacgcgcaac
    20041 gttaccgatg atggaacaat aaaatcaacg tatgaaaaaa cgtatcccca ctctcctggc
    20101 caccatgatt gccaccgccc tttatagtca acagggactg gcagccgacc tcgcctcaca
    20161 gtgcatgttg ggcgtgccaa gctatgaccg tcctctggta cagggcgata ccaatgactt
    20221 acccgtgact atcaatgctg accacgcgaa aggggactac ccggatgacg ccgtgtttac
    20281 tggcagcgtg gatatcatgc agggtaacag ccgtctgcag gccgacgaag tgcagctcca
    20341 tcaaaaagag gcaccaggac aaccggagcc ggtacgtacc gttgatgcgc tcggtaatgt
    20401 ccattacgac gataaccagg tgatcctcaa agggccgaaa ggctgggcga atctgaacac
    20461 caaagatacc aacgtctggg aaggtgatta ccagatggtg ggtcgccagg gtcgcggtaa
    20521 agcggacctg atgaaacaac gtggcgaaaa ccgctatacc attctggata acggtagctt
    20581 tacctcctgt ctgccgggtt ctgacacctg gagcgtggta ggtagcgaaa ttattcatga
    20641 ccgcgaagaa caagttgcgg agatctggaa cgcccgcttt aaggtgggtc cggtaccgat
    20701 cttttatagc ccctatttgc agttgccggt gggtgacaaa cgtcgctctg gtttcttgat
    20761 cccgaacgcc aagtacacca ccaccaacta ctttgagttc tacctgccat attactggaa
    20821 catcgcgcca aatatggatg ccaccatcac gccgcattat atgcatcgtc gtggcaacat
    20881 catgtgggag aacgaattcc gctacctctc ccaggcgggc gctggcttga tggaactgga
    20941 ctatctgcct tcagataaag tctatgaaga tgaacacccg aacgatgaca gttcacgtcg
    21001 ttggttgttc tactggcaac actccggggt catggatcaa gtgtggcgtt tcaacgtcga
    21061 ctacaccaag gtcagcgatc ctagctactt caatgatttc gataacaagt acggttccag
    21121 tactgacggc tacgcaacgc aaaaattcag cgttggctat gcggtgcaaa acttcaatgc
    21181 caccgtttca accaagcagt tccaggtctt tagcgagcag aacaccagta gctactcggc
    21241 agagccgcag ttagacgtta actactacca gaatgatgtt ggtccgtttg atacgcgtat
    21301 ttacggccag gcagtgcact ttgttaacac cagagacgac atgcctgaag caacccgtgt
    21361 tcacctggaa ccgaccatca atttgccgct ctctaataac tggggcagca tcaataccga
    21421 agcgaagttg ctggcaaccc attatcagca aaccaatctt gactggtata actccagaaa
    21481 cacgaccaag ctggacgaat ccgttaaccg cgttatgccg caattcaaag ttgacggcaa
    21541 aatggtcttt gaacgcgata tggaaatgct ggctccgggt tatacccaaa cgctggaacc
    21601 gcgcgcgcag tatttgtacg tgccgtatcg cgatcagagc gacatctata actacgactc
    21661 gtctctactg caatctgact actctggcct gttccgggac cggacttacg gcggtcttga
    21721 ccgtattgcc tccgctaacc aggtgacgac cggtgtcaca tctcgcatat atgatgatgc
    21781 tgccgttgaa cgttttaata tttccgttgg tcaaatctac tatttcacgg agtctcgcac
    21841 tggcgatgac aacataacat gggagaatga cgacaaaacg ggctcactgg tgtgggcagg
    21901 cgatacttac tggcgtatct ccgagcgttg gggattgcgt ggcgggattc agtacgatac
    21961 acgtctggat aacgtagcga ccagtaactc cagcattgaa taccgtcggg atgaagaccg
    22021 tctggtacag ctgaattacc gttacgccag cccggaatat attcaggcta cgctgcctaa
    22081 gtactattcc actgctgagc aatataagaa tggtatttcg caggtaggtg ctgtcgccag
    22141 ctggccaatt gccgatcgtt ggtccattgt tggggcctac tactacgaca ccaatgcgaa
    22201 caagcaagcc gactctatgt taggtgtgca atacagctcc tgctgctatg caattcgcgt
    22261 cggttacgag cggaagctga acggttggga taacgataaa caacatgcgg tatatgacaa
    22321 cgcaatcggc tttaacatcg aacttcgcgg cctgagctcc aactacggtc tgggtacgca
    22381 agagatgctg cgttcgaaca ttctgccgta tcaaaacact ttgtgatttg ttgatttacc
    22441 acgtaatccg cagtgcggtt aattgaaatg gaaaaagtat gaagaactgg aaaacgctgc
    22501 ttctcggtat cgccatgatc gcgaatacca gtttcgctgc cccccaggta gtcgataaag
    22561 tcgcagccgt cgtcaataac ggcgtcgtgc tggaaagcga cgttgatgga ttaatgcagt
    22621 cggtaaaact gaacgctgcg caggcaaggc agcagcttcc tgatgacgcg acgctgcgcc
    22681 accaaatcat ggaacgtttg atcatggatc aaatcatcct gcagatgggg cagaaaatgg
    22741 gagtgaaaat ctccgatgag cagctggatc aggcgattgc taacatcgcg aaacagaaca
    22801 acatgacgct ggatcagatg cgcagccgtc tggcttacga tggtctgaac tacaacacct
    22861 atcgtaacca gatccgcaaa gagatgatta tctctgaagt gcgtaacaac gaggtgcgtc
    22921 gtcgcatcac catcctgccg caggaagtcg aatccctggc gcagcaggtg ggtaaccaaa
    22981 acgacgccag cactgagctg aacctgagcc acatcctgat cccgctgccg gaaaacccga
    23041 cttctgatca ggtgaacgaa gcggaaagcc aggcgcgcgc cattgtcgat caggcgcgta
    23101 acggcgctga tttcggtaag ctggcgattg ctcattctgc cgaccagcag gcgctgaacg
    23161 gcggccagat gggctggggc cgtattcagg agttgccggg tatcttcgcc caggcattaa
    23221 gcaccgcgaa gaaaggcgac attgttggcc cgattcgttc cggcgttggc ttccatattc
    23281 tgaaagttaa cgacctgcgc ggcgaaagca aaaatatctc ggtgaccgaa gttcatgctc
    23341 gccatattct gctgaaaccg tcgccgatca tgactgacga acaggcccgt gtgaaactgg
    23401 aacagattgc tgccgatatc aagagtggta aaacgacttt tgctgccgca gcgaaagagt
    23461 tctctcagga tccaggctct gctaaccagg gcggtgatct cggctgggct acaccagata
    23521 ttttcgatcc ggccttccgt gacgccctga cccgcctgaa caaaggtcaa atgagtgcac
    23581 cggttcactc ttcattcggc tggcatttaa tcgaactgct ggatacccgt aatgtcgata
    23641 aaaccgacgc tgcgcagaag gatcgtgcat accgcatgct gatgaaccgt aagttctcgg
    23701 aagaagcagc aagctggatg caggaacaac gcgccagcgc ctacgttaaa atcctgagca
    23761 actaatggtt aaaacccaac gtgttgtgat cactcccggc gagcccgccg ggattggccc
    23821 ggacttagtt gtccagcttg cacagcgtga gtggccggtc gaactggttg tttgtgccga
    23881 tgccactctc cttaccgacc gggcagcgat gctcggtttg ccgctcaccc tccgccctta
    23941 ttcccccaac tcccctgcac aaccgcaaac tgcgggcaca ttaacgctac ttcctgtcgc
    24001 gctacgcgaa tctgtcactg cggggcagtt agcgattgaa aatgggcatt acgtggtgga
    24061 gacgctggcg cgagcgtgtg atggctgtct gaacggtgaa tttgctgcgc tgatcacagg
    24121 ccccgtgcat aaaggcgtca ttaacgacgc aggtattccg tttactggtc ataccgagtt
    24181 tttcgaagag cgctcgcagg cgaaaaaagt ggtgatgatg ctggcgaccg aagaacttcg
    24241 cgtggcgctg gcaacgacgc atttaccgct gcgcgatatc gcagatgcta tcacccctgc
    24301 acttttgcac gaagtgattg ctattttgca tcacgatttg cgtacaaaat ttggtattgc
    24361 cgaaccgcgc attctggtct gcgggctgaa tccgcacgcg ggcgaaggcg gtcatatggg
    24421 tacggaagag atagacacca ttattccggt gctcaacgag ctgcgggcgc aggggatgaa
    24481 actcaacggg ccgctgcctg ccgataccct gtttcagccg aaatatctcg ataacgccga
    24541 cgccgtgctg gcgatgtacc acgatcaggg tcttcccgtg ctaaaatacc agggcttcgg
    24601 gcgcggtgtg aacattacgc tgggcctgcc ctttattcgc acatcagtgg accacggcac
    24661 cgcgcttgaa ctggcgggac gtggcgaagc cgatgtcggc agttttatta cggcgcttaa
    24721 tctcgccatc aaaatgattg ttaacaccca atgaataatc gagtccacca gggccactta
    24781 gcccgtaaac gcttcgggca aaactttctc aacgatcagt tcgtgatcga cagcattgtt
    24841 tctgccatta acccgcaaaa gggccaagca atggtcgaaa tcggccccgg tctggcggca
    24901 ttgaccgagc cggtcggcga acgtctggac cagctgacgg tcatcgaact tgaccgcgat
    24961 ctggcggcac gtctgcaaac gcatccattc ttaggcccga aactgacgat ttatcagcag
    25021 gatgcgatga cctttaactt tggcgaactg gccgagaaaa tgggtcagcc gctgcgtgtt
    25081 ttcggcaacc tgccttataa catctccacg ccgttgatgt tccatctgtt tagctatact
    25141 gatgccattg ccgacatgca ctttatgttg caaaaagagg tggtgaatcg tctggttgca
    25201 ggaccgaaca gcaaagcgta tggtcgatta agcgtcatgg cgcaatacta ttgcaacgtg
    25261 atcccagtgc tggaagtacc gccgtcagcc tttacaccac cacccaaagt ggattccgcc
    25321 gtcgtgcgcc tggttcctca tgcaacgatg cctcacccgg ttaaagatgt tcgcgtgttg
    25381 agccgcatca ccactgaagc ctttaaccag cgtcgtaaaa ccattcgtaa cagcctcggc
    25441 aacctgttta gcgtcgaggt gttaacggga atggggatcg acccggcgat gcgagcggaa
    25501 aatatctctg tcgcgcaata ttgccagatg gcgaactatc tggcggagaa cgcgcctttg
    25561 caggagagtt aacgatgatc aattcgcccc gagtgtgtat tcaggttcaa agcgtctaca
    25621 ttgaggcaca atcttcacct gataatgaac gttacgtttt tgcttatacc gtaaccatac
    25681 gcaatctggg gcgagcgcca gtgcagttgt tggggcgtta ctggctgatc accaatggca
    25741 atggccgtga aaccgaagtc cagggcgaag gagtggttgg cgtccagcca cttatcgcgc
    25801 ctggcgaaga gtaccagtac accagcggtg caatcattga aaccccgctg ggcaccatgc
    25861 agggtcacta cgaaatgatc gatgaaaatg gcgtcccttt cagcatcgac attcccgtat
    25921 tccgactcgc cgttcccaca ctcattcatt aaaagaatat ggcgacatac cttattggcg
    25981 acgttcatgg ttgttacgat gaactgatcg cattgctgca taaagtagaa tttacccctg
    26041 ggaaagatac cctctggctg acgggcgatc tggtcgcgcg cgggccgggt tcgctggatg
    26101 ttctgcgcta tgtgaaatcc ttaggcgaca gcgtacgtct ggtgctgggc aatcacgatc
    26161 tgcatctgct ggcggtattt gccgggatca gccgcaataa accgaaagat cgcctgacac
    26221 cgctgctgga agcgccggat gccgacgagc tgcttaactg gctgcgtcgc cagcctttgc
    26281 tgcaaatcga cgaagagaaa aagttggtga tggcccacgc agggatcacg ccgcagtggg
    26341 atctacagac cgccaaagag tgcgcgcgcg atgtagaagc ggtgctatcg agtgactcct
    26401 atcccttctt tctcgacgct atgtacggcg atatgccaaa taactggtca ccggaattgc
    26461 gggggctggg aagactgcgt tttatcacca acgcttttac ccgtatgcgt ttttgcttcc
    26521 cgaacggtca actggatatg tacagcaaag aatcgccgga agaggcccct gccccactga
    26581 aaccgtggtt tgcgattcct ggccctgtcg ctgaagaata cagcatcgcc tttggtcact
    26641 gggcatcgct ggagggcaaa ggtacgccgg aaggtatata cgcgctggat accggctgct
    26701 gctggggtgg tacattaacc tgcctgcgct gggaagataa acagtatttt gtccagccgt
    26761 cgaaccggca taaggatttg ggcgaagcgg cggcgtctta aacacagcct gatataggaa
    26821 ggccggataa gacgcgaccg gcgtcgcatc cggcactagc cgtaaattct atacaaaatt
    26881 accgccgctc cagaatctca aagcaatagc tgtgagagtt ctgcgcatca gcatcgtgga
    26941 attcgctgaa taccgattcc cagtcatccg gctcgtaatc cgggaaatgg gtgtcgcctt
    27001 ccacttctgc gtcgatatgc gtcagataca gtttctgcgc ttttggcagg aactgctcat
    27061 aaacgcgacc gccgccaatc accatgattt ctggtacgtc accacacgcc gcgatggctt
    27121 catccaccga cttcacccac gttacgcgat cgtccgtacc cggttgactg ctgaggataa
    27181 tatttttgcg tcctggcaac ggacgaccga ttgattccca agtatggcgg cccataatca
    27241 cgggtttatt taaggtgttg cgtttaaacc aggcgagatc ggcaggcagg ttccacggca
    27301 tggcgttttc catgccgata acgcgatcta ccgctaacgc cgcaatcaga ctgatcattg
    27361 agatttcccg ataaaaaaaa ttgtcgccac tatacgtaaa gcgtaaaccg tcgtcgactg
    27421 gtgcgaggat gatgttgagg aaaattttat attctgctgg cgagtccacg ctctctccct
    27481 ggactcgccg cattacaatg aaacaaaaac aaacagttag ctgtaaagtg tgatttacgt
    27541 cactctttat taggatgagg gtttcgtttc cggttcatcc gccatgttgc cggtatgttt
    27601 accttcttcg gttccctgcc agccatgtcg ttgaattaat gacagatgtt cgcggtcctc
    27661 ggtaatgatc tcacttaaca tcgcgctggt gcgtttatag accgccgcgc gggctttggt
    27721 gtcgttctca accattgcca tctcttccac catctgaata ttaaagcggc ggaacacatc
    27781 ggcacgttct cgcgcttcat acggccccag acctaaactt tccagtgcca gacgcccggt
    27841 tttcagcgca ccttcgaagg tttcacgctc cggcttttca acgcctgcct gacgcaaacg
    27901 aatgtagtgg tcgacatcgc gggcgcgggc aataatctgc aaatgcggga agtgttcttt
    27961 caccatctct gtcagttgca ggttggtttg cggatcgtcg atggcgttaa tcagcacttc
    28021 cgctttcgcc gctcccgcag attccagtaa atccatccgc gtggcatcgc cataaaacac
    28081 tttcatacca aatttacgca aggtttcgat atggtccgga tcgtgatcga gcaccaccat
    28141 tttcaccccg ctggagagca gtaaacgtcc ggtgatctgc ccaaaacgac cgaatccggc
    28201 aatgattacc cgcggctgtt cttcgtcgat ctcatcagct tcacgcgcct cttcagtaga
    28261 agattgctca aggcgattga ggatcaccag cagaatcggc gttgccgcca tcgacagcgc
    28321 caccgccagg gtcagcgatt tcgcccactc cggttccagc acattcgcca tctgcgccgt
    28381 gccaaatacc acaaaggcaa actcactgcc ctgccctaac aacaccgcaa accaacgacg
    28441 ctgtttattt ggcacttgca acggtcgggc aatcagccac agcatggcga ttttgatgat
    28501 gaggaaaccg agcagcaaaa tgacaatgcg caatgggttt tcaagcagcg tgccaaagtc
    28561 tatcgacatg ccaacaccga tgaaaaacag ccccaacagc aaacctttaa atggttcgat
    28621 atcgctctcc agcgcatgac ggtattccga gcttgccagc agtacgcccg ccagaaacgc
    28681 gcccatcgcc atcgacaagc cgacctcttc cagcagcaaa ccaaagccaa acacgaggaa
    28741 taacgccacg gcactaaaca cttcccgcaa gccagagcgg gctacaaaac gcagcgccgg
    28801 acgcgtgaca tagcgcccca gcaataccac cagcaccagc gcacccgcca cttttaacgc
    28861 cgagagagca aatgcgccca tcgtcgtcga ggcactgctc gttgccagta gcggaatcat
    28921 cgccaccagc gggatcgccg cgatatcctg gaacagcagc accgcaaagg cactgcgacc
    28981 catttgcgtc accatcagat tgcgttcatt catcgcctgc atggcaatcg ccgtagagga
    29041 gagcgccagc gtcatgccga tcaattccgc gacctgccag tgcagcccaa gtaacatgca
    29101 gaacagcccc agcaggccgc cgcaaatcac catctgcaat gcgccaccgc cgaacactgc
    29161 cgcacgcagc ttccacagcc tttgtggatc gagttcgagg ccgataataa acagcatcag
    29221 caccacccca atctcggcaa agtgcagaat agattcggca tcggtcacca gtcgcagccc
    29281 ccacgggcca ataatgcagc cggcgatcag gtagccaagt accgatccca gaccaagacg
    29341 taccgcaatg ggtacaatca gcgctgccga accgagataa atcagcgcct gaatcagcgt
    29401 atggctatcc atgatgggcc tcctgccatt ccagcagacg ttgcttatag tgacgcgcct
    29461 gcccttcgag ggtttcgtcg tcacaaataa aggtgcagtg catggcaaac ggtggcagcc
    29521 agttcagccc gcagtagatt gccgtcgcct gtagcggctg cgacagcaca tcaaagcccg
    29581 gatgcgcacc aatttcaaaa tggctttccc cgccgccggt cgtcaccgcc cacagcaaat
    29641 atttgccatg cagcgccttg ccgccgtgac cgtaagccca gccgtgcgac aaaactttat
    29701 cgatccaaag tttaagaagc ggaggaatgc tgtaccactg catcggatgc tgccagacga
    29761 tcagatcggc gcgagacagc gcctcctgct cggcggcaat atcgatattg aagtcaggat
    29821 agagttgata aagagagcga atttcgacgc cttccagcgt ccttgcctgt tcaagcatcc
    29881 gtttattcgc atgggaatga tgcggatacg gatgcgcata aattataaga atcataggta
    29941 gcccgcgtga ttacgccttt gttttcccac aaagtgtagt catatattca cgcggcttat
    30001 agtcaataat attgattcag aaaagagaga aatctgaaca atttacccca ttctctgccc
    30061 gcggatcgtc atattgctgg tttgcgccag tgacatccct cgagtctccg gggcaaacgc
    30121 tacggaaatc aacaagccaa acagcgagat acccgcctcc attagcatcg tgttactgat
    30181 accgtaatta ttgataaaga tcggcagtgc ccaggtcgaa acaatggtgc caatacggct
    30241 taaggacata atcacgccca cggcagaggc gcggatatct gtcgggaaga gttcattagg
    30301 atagagccac tgcaaattac ccggcccgcc agagaaaaag gcatacaccg caaaggccat
    30361 cactaccagc cagatcccca tatccgggat cagccccaaa accgccagcg ccagcgtcat
    30421 catggcaaag ctgccaatca acaatggacg ccgtccggca gtgtttaacc acagcatcgg
    30481 cggaatacag ccgagcataa agaacaggct aatcaccacg ttccccagcg ccgcgttttt
    30541 gccaacaccc aatcccaaca aaccaacgat ttgcgggcca aaggtgtaaa tggcgaacat
    30601 tgggatcacc tggcaggtcc agatggcggc aacaaacaga acaaaaggaa aatggcggcg
    30661 attaaacaga tcgcgaaaac gggtttgcta cggctgctct tcatcgaaag ccaccggttc
    30721 gccaaacagt tttatcatca tctcttcgca ctcttttact cgccctttgc gtaataacca
    30781 gcggggagat tcaggcagtt cgaatcgacc aatcaaaatc aacaaacagg ggatcgccgc
    30841 gctacccagc atccagcgcc agccgccttc cacatcataa agccagtagc cgaccagatc
    30901 ggcgcaggtc gcgccgacat accacatggc ggcgataaag ctgatggaaa atgcccgctg
    30961 acgggtactg gagaactcgg tgatcattga ggtggcgatg ggataatctg caccgatgac
    31021 aatgccgata agtacccgca tcaccaacag ttcgacgggg gatgaaacaa acatcgtcgc
    31081 caccgatatc acgccgatgg cgatgatatc aatgaggaac attttgcgcc gtcctacttt
    31141 atcggaaata tagccaaaca gcgatgtgcc aacgaacagc ccggcgagcg ttcccgcgcc
    31201 caacaagcca atccagtcag cgtccagttt tagcgccgga gtcagttgct ccagcgccac
    31261 gccaatcatt accagtacat aaccatccag aaacggacca ccgcttcccc acagcaaaat
    31321 gcggcggtga attgaggaga atttgagatc gtcaaagttt ctggacggtt gcatgggtta
    31381 ttcctgtttt tttgcaatga gagtcagtac ggcgttacgt atcaaaccaa catcagccgt
    31441 aacggaactc cacaccaaag gtgccgcgcg ggtattccca ctgttccagc gccgacccca
    31501 gccccagaat gcgacaggtg ccgcactcca gacatccggc gtaatcgaag cgcacactgc
    31561 cgtcatcctg cttcttgtac agacctgcgg ggcacgcttt caccagcagc tccagggcct
    31621 gtttatcggc atcggccttt acaacaatgt gcggatgctc ttcatcgaca ttgaatttat
    31681 tgacgcccag tttgacgtcc acattgacgg gagaagtcat aaaacggtca ctcctttcat
    31741 gccatccttg atcagattga tgaagcccac tttcttgccg tggcggagga ttttcttgcg
    31801 catcagttcc ggcgcactgc cgtcaatggt gaacaggtca cgcgccacgc ccaccgccag
    31861 ttccgggtag ccgctaaaca tgcgtgggtt atcaaggaag gccggtagtt tctggtacat
    31921 acgcatatcg cgcagcgggc cactctcaag atgctgacga tattccgcca gtttttgctt
    31981 actgaaatcg tcgcttttca tcgctgaaag cacggttttt gctgcggctt ccccggcggc
    32041 aatcgccaga tccataccgc gaatggtaaa accgaggttc atacacattc cggcggcatc
    32101 accggcaatc aatacgccgt caccaaccaa ctccggcagc atgttgatgc ctgcttccgg
    32161 cactacgtga gcggaatatt ccaccagctt gccgcccgcg atcagcggtg ccactgccgg
    32221 atgctgtttg aaatcttcca gcatttgcgg caccgatttt tttgcgtcat gcagatgatg
    32281 cagaccacaa accagcccca gcgacagggt attttcattg gtataaagga agccgccgcc
    32341 catcaggcca tcggtgggtg atcccgcaaa cagacaagcc gcgccctgat taccctgcaa
    32401 ctgaaaacgg tcttcgatta ccgacttcgg taactcgatc agttccttca cgccaaccgc
    32461 cacatccgtc ggtttaacgc gttttgccat ccccagcttt tcggcaagga tggagttcac
    32521 tccatcagca aggatcaccg ttttcgcttc aatcacatcg ccatcggctt ctacaccgac
    32581 gactttgcca tcgcgctgta cgaggttatc gacgcggatc ccggtaatta actgcgcgcc
    32641 cgcttcttcg gcctgctcca tcagccaggc atcaaattta ctgcgcaaaa cggagtaaga
    32701 acgctgggat ggcgaggttt cgtcaccgtt gcagtagtcc atagtcatcg ctgacttttc
    32761 cgtcataaac gcgagttttt catgggtgat caggcgttct accggggcgg attcggcgaa
    32821 accaggaata atgtgttcca ggctgtgggc atagagacgc ccgccggtga cgttcttggc
    32881 acctgcggaa ttgccacgct cgataactaa cacttgcgcc ccttcgcggg cgagcaccag
    32941 tgcggcaacc gaaccggcaa gcccagcacc gacgatgatg gcgtcaaaga tatcttcgga
    33001 cataagagct ccctgtcagc ggcagggcca aaatgcagcc ctgccagagt ggatcaacgc
    33061 gctaaagctg cggtcagcgc cggaaggatc ttcacggcgt caccaacaat gccgtaatcc
    33121 gcgtactgga agatcggcgc atttttatct ttattgatgg cgaaaatggt ttgcgatgca
    33181 ttagcaccaa ccatgtgctg gatctgcccg gagatcccca ccgccaggta cagttcaggt
    33241 ttcagcatca ggttggagat accgacatag cgttcgtgct ccatccattt ttcgttttcc
    33301 gccaccggac gagaacaggc caactccgca cctatcgcct tgcaaagctg ttctgccagc
    33361 gcaatgttct ctttgctgcc aataccgcga ccgacgctga ccaccagacg ggctttgtcg
    33421 agatcgacgc tgttgctctg gcgcgcctgg gttgccgtgc gggtaatcgc cacagccgga
    33481 gcctgccact ccacggtgtg cgtttcgcca gtgcgtgacg cgtctggctg agccgcatcg
    33541 aacgtgccgc tgctgatggt cagtaccgca tacggcgtgg caatgcgttc ttcgccaatc
    33601 gccagaccac cgtaaaccat atgtttcact gttgctttac cgtcctgtac gctgacggtg
    33661 ctggcatcgt tagacaccgc cgctttaagg cgataaccca gttttgccgc cagtaatttg
    33721 ccgcgacggg tgtttggcag cagcaccagg ccgtctgcgc cgtgctggcg aatagtgtca
    33781 gccatgacac cggcgtaatc ttcgatcatc cgatcgtccg gtttgccgtt taatttccag
    33841 acatgattag cgccgagctg gattgcctgt gcgccgtcgg catcattgag gacaaaggtg
    33901 ttgatttgat tagctaaagc ctgcgcaccg ttcatcagtt ccggcagacg agaaggggta
    33961 tcgctgaata cccagacttg agaaaacgtg ttcatagcat cccctgtaat taaatgactt
    34021 tgcgaagatt ttcagcaaat gcggcgatct gttcttcgcc gtcgccttca atcacgatgc
    34081 gctgacgttc gcgctgtttc ggcgcggcaa cctgttgttc tgaccaggct gcctctgcgt
    34141 taaaaccaat atccgccgcc gaccatacct ggacgggctt tttcgccgcg ccgagaatgg
    34201 ctttcatcga aggaatttgt ggggaattga tatcagtgga aacagcaaca accgcaggca
    34261 gcggaatgct taaggtttcg gtttcatctt ccagttcgcg ctcaacggtg agggtatctg
    34321 ccgtcaggga gataattttg ctgacgccgt taactgccgg aatattgagg atttcgccca
    34381 ccagcagacc aacctgctgg gcataaaggt cggaagaacc atcgccacag aggatcaaat
    34441 caaagcctgc tttctgggcg gctgcagcca gtgcgctcgc cgtttgttgc ggcagtgcct
    34501 gctcgaactg gtcatcaatc accacaatca gttcatccgg gccgcgcgat agtacatctt
    34561 tacgcccttt ggcgttggtc agggctttac cgcccacact taaggccgtc acctgcgcct
    34621 ctgctgcctg ttgctttagc tggcaagccg cttcaatagc gttcagatcg tattggctta
    34681 ttttggcatc ggctttgctg aagtctaatg aaccatcagc attattgacc gcaatatcct
    34741 gttcatcagg cacgcactta tagcaagtaa taatcttcat tgcatctcca gaaatcatga
    34801 aggaaacatc gttcatatcg gtggtgttaa ttacagatat cacgcccttt aatttggaaa
    34861 taaaacttta atcaccaata ttgaaaatgt cacacgcaga taaatccaac tttcaatatt
    34921 gttaagttcc tcaccaatat tgaaaacatg gcgtagaaaa aagaaatttt caatattgct
    34981 ttatggatca ccaatattga aagtcagatg ctattttttt actcacaaca caacataaca
    35041 aactgattaa taatcaaaat taacgagaaa agcgccctct attcacccga aaaaaaatgt
    35101 gataacaatc acagaatgca gcttattgaa tacccattat gagttagcca ttaacgcgtc
    35161 cacgaggtta ataataatta tatcaaatgt taacaaaaat aaaacaaacg ggaacgcaaa
    35221 ataaatattc gttttcacag tggaattaac tcatgaagaa tgaaaagaga aaaacgggaa
    35281 tagaaccgaa ggttttcttt ccgccgttaa taatcgtcgg catactttgt tggcttacag
    35341 tcagagatct ggatgcagcg aatgtcgtta ttaatgctgt attcagttac gtcaccaatg
    35401 tatggggatg ggcatttgaa tggtatatgg tggtgatgct tttcggttgg ttctggctgg
    35461 tgtttggccc gtatgccaaa aagcgtttag gtaacgaacc acctgaattt agtaccgcca
    35521 gttggatctt tatgatgttc gcctcctgta cgtctgctgc cgtactgttc tggggatcga
    35581 ttgagatcta ctactacatc tccaccccgc cgtttggctt agaaccgaac tcgacagggg
    35641 cgaaagagtt ggggctggct tacagcttgt tccactgggg acctctgccg tgggccactt
    35701 acagcttcct ttcagtcgcc ttcgcttact tcttctttgt ccgcaaaatg gaagtgattc
    35761 gccccagctc gacactggtg ccgctggtag gtgaaaaaca cgccaaaggg ttgttcggca
    35821 ctatcgtcga caacttctat ctcgtcgcct tgatcttcgc gatgggtacc agtctgggcc
    35881 ttgccacgcc gctggtgacc gagtgtatgc aatggttgtt tggcattccg cataccctgc
    35941 aactggacgc tatcatcatt acctgctgga ttatcctcaa cgccatttgc gtcgcttgcg
    36001 gtctgcaaaa aggggtacgt atcgccagtg acgtgcgtag ttacctgagc ttcctgatgc
    36061 tgggttgggt gttcattgtc agcggtgcca gcttcatcat gaactacttc accgattcgg
    36121 tggggatgtt gctgatgtat ctgccgcgca tgttgttcta taccgatccc atcgctaaag
    36181 gcggcttccc gcagggctgg accgtgttct actgggcatg gtgggtgatt tatgctatcc
    36241 agatgagtat cttcctcgcc cgcatctccc gtggtcgtac tgtgcgtgaa ctgtgcttcg
    36301 gcatggtgct ggggctgaca gcgtcaacct ggatcctgtg gactgtactc ggtagtaaca
    36361 ctctgctgtt gatagataaa aacatcatca acattccaaa tctgatcgaa cagtacggtg
    36421 tggcgcgcgc catcattgaa acctgggccg ctctgccact cagcaccgcc accatgtggg
    36481 gcttcttcat cctctgcttt attgccaccg ttacactggt taacgcctgc tcttataccc
    36541 tggcgatgtc cacttgccgc gaagtacgcg atggtgaaga accacctctg ctggtgcgta
    36601 tcggttggtc aattctggtt ggcattatcg gtattgttct gctggcgctc ggcggcctga
    36661 aaccgattca aaccgccatt atcgccggag gatgcccgct gttcttcgtc aacattatgg
    36721 tgacgctctc ctttattaaa gacgcgaaac agaactggaa agattaatta acccccaaaa
    36781 tatcaagagg ttgaaagatg gattttaatt taaatgatga gcaggaactg tttgtcgccg
    36841 gtatccgcga actgatggcc agcgaaaact gggaggccta ttttgccgag tgcgaccgtg
    36901 acagcgtcta cccggaacgt tttgtcaaag cactggcgga tatgggtatc gacagtctgc
    36961 tgatccctga agagcacggt ggtctggacg cggggtttgt tactctcgcc gccgtgtgga
    37021 tggagctggg acgtctgggg gcaccaacct atgtgctgta ccagttgccg ggcgggttca
    37081 acaccttcct gcgcgaaggc acacaagagc agatcgacaa aattatggct ttccgcggca
    37141 ccggtaagca gatgtggaac tcagcgatta ccgaaccggg cgcgggctcc gacgtgggta
    37201 gcctgaaaac gacttatacc cgtagaaatg gtaagattta tcttaatggt agtaagtgtt
    37261 ttattaccag cagcgcctac accccgtaca tcgtggtgat ggcgcgcgac ggggcttctc
    37321 cggacaaacc tgtctacacc gaatggtttg ttgatatgag caaaccgggc atcaaagtga
    37381 ccaaacttga aaagctcggt ctgcgtatgg atagctgctg tgaaatcacc tttgacgacg
    37441 tggaactgga cgagaaagac atgttcggtc gggaaggtaa cggctttaac cgcgtcaaag
    37501 aagagttcga ccatgaacgt ttcctggtag ccctcaccaa ctacggtacg gcgatgtgcg
    37561 cttttgaaga tgcggcgcgc tacgccaatc agcgcgtgca gtttggcgag gctattggtc
    37621 gtttccagtt gattcaggaa aaattcgccc acatggcgat caaattaaac tccatgaaaa
    37681 acatgctgta tgaagcagcg tggaaagcag acaacggcac catcacctct ggcgatgcag
    37741 cgatgtgcaa atacttctgc gccaatgcgg catttgaagt tgtggatagc gcaatgcagg
    37801 tgctgggcgg tgtcgggatt gcgggcaacc accgcatcag ccgcttctgg cgtgatctgc
    37861 gtgtagaccg cgtctccggg ggatctgacg aaatgcagat cctgacgctg ggtcgcgcgg
    37921 tgctgaagca ataccgctaa gtccgtgcgc tgcctgatgc gacgctaatg cgtcttatca
    37981 ggcctacggg agcacgaatg taggccggat aaagcgttta cgccgcatcc ggcagtcatg
    38041 cactacataa atttttcagg agctaataat ggatcatcta cccatgccga aattcgggcc
    38101 gttggccgga ttgcgcgttg tcttctccgg tatcgaaatc gccgggccgt tcgccgggca
    38161 aatgttcgca gaatggggtg cggaagttat ctggatcgag aacgtcgcct aggccgacac
    38221 cattcgcgtt caaccgaact acccgcagct ctcccgccgc aatttgcacg cgctgtcgtt
    38281 aaatattttc aaagatgaag gccgcgaagc gtttctgaaa ttaatggaaa ccaccgatat
    38341 cttcatcgaa gccagcaaag gtccggcctt tgcccgtcgt ggcattaccg atgaagtact
    38401 gtggcagcac aacccgaaac tggttatcgc tcacctgtcc ggttttggtc agtacggcac
    38461 cgaggagtac accaatcttc cggcctataa caccatcgcc caggccttca gtggttacct
    38521 gattcagaac ggtgatgttg accagccaat gcctgccttc ccgtataccg ccgattactt
    38581 ttctggcctg accgccacca cggcggcgct ggcagcactg cataaagtgc gtgaaaccgg
    38641 taaaggcgaa agtatcgaca tcgccatgta tgaagtgatg ctgcgtatgg gccagtactt
    38701 catgatggat tacttcaacg gcggcgaaat gtgcccgcgc atgagcaaag gtaaagatcc
    38761 ctactacgcc ggttgcggtc tgtataaatg cgccgacggc tacatcgtga tggaactggt
    38821 gggcattacc caaattgaag agtgctttaa agatattgac ctcgcacatc tactcggtac
    38881 gccggaaatc ccggaaggca ctcagcttat ccaccgtatc gaatgccctt acggcccact
    38941 ggtagaagaa aaactcgatg cctggctggc ggcacatacc atcgcagaag ttaaagaacg
    39001 ctttgccgaa ctgaatatcg cctgtgccaa agtactgacc gtaccggaac tggaaagcaa
    39061 tccacagtat gttgcccgtg aatcaatcac tcacatggca tggacacggc tgccattttg
    39121 aaaaatatcg gctacagcga aaacgacatt caggagttgg tcag
//
LOCUS       DAEEKQ010000022        38337 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.022, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000022 DAEEKQ010000000
VERSION     DAEEKQ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38337
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.022"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            59..1423
                     /locus_tag="KLK57_001101"
     CDS             59..1423
                     /locus_tag="KLK57_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0891534.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSIVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGVKEVLIAEEEHSAYVKIDSKVTNRFEVEQA
                     IRQA"
     gene            complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="KLK57_001102"
     CDS             complement(1551..2042)
                     /gene="yajQ"
                     /locus_tag="KLK57_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HBE0891535.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2210..3121
                     /gene="panE"
                     /locus_tag="KLK57_001103"
     CDS             2210..3121
                     /gene="panE"
                     /locus_tag="KLK57_001103"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HBE0891536.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3084..3674
                     /gene="yajL"
                     /locus_tag="KLK57_001104"
     CDS             3084..3674
                     /gene="yajL"
                     /locus_tag="KLK57_001104"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HBE0891537.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKTHEVASQLVMAAGIYNYYE"
     gene            complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="KLK57_001105"
     CDS             complement(3728..5176)
                     /gene="thiI"
                     /locus_tag="KLK57_001105"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HBE0891538.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDEKQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5382..5624
                     /gene="xseB"
                     /locus_tag="KLK57_001106"
     CDS             5382..5624
                     /gene="xseB"
                     /locus_tag="KLK57_001106"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HBE0891539.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDTSLPPFTPDNE"
     gene            5624..6523
                     /gene="ispA"
                     /locus_tag="KLK57_001107"
     CDS             5624..6523
                     /gene="ispA"
                     /locus_tag="KLK57_001107"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HBE0891540.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDADMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6548..8410
                     /gene="dxs"
                     /locus_tag="KLK57_001108"
     CDS             6548..8410
                     /gene="dxs"
                     /locus_tag="KLK57_001108"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HBE0891541.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVTAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            8465..9439
                     /gene="yajO"
                     /locus_tag="KLK57_001109"
     CDS             8465..9439
                     /gene="yajO"
                     /locus_tag="KLK57_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HBE0891542.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALKLQKQHGWAQFISMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="KLK57_001110"
     CDS             complement(9493..10011)
                     /gene="pgpA"
                     /locus_tag="KLK57_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HBE0891543.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="KLK57_001111"
     CDS             complement(9989..10966)
                     /gene="thiL"
                     /locus_tag="KLK57_001111"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HBE0891544.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="KLK57_001112"
     CDS             complement(11044..11463)
                     /gene="nusB"
                     /locus_tag="KLK57_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HBE0891545.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="KLK57_001113"
     CDS             complement(11483..11953)
                     /gene="ribE"
                     /locus_tag="KLK57_001113"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HBE0891546.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="KLK57_001114"
     CDS             complement(12042..13145)
                     /gene="ribD"
                     /locus_tag="KLK57_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HBE0891547.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGGVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSSEWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="KLK57_001115"
     CDS             complement(13149..13598)
                     /gene="nrdR"
                     /locus_tag="KLK57_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HBE0891548.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13749..14288
                     /locus_tag="KLK57_001116"
     CDS             13749..14288
                     /locus_tag="KLK57_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HBE0891549.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            14587..15471
                     /gene="tsx"
                     /locus_tag="KLK57_001117"
     CDS             14587..15471
                     /gene="tsx"
                     /locus_tag="KLK57_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HBE0891550.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYDAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15648..15995)
                     /locus_tag="KLK57_001118"
     CDS             complement(15648..15995)
                     /locus_tag="KLK57_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HBE0891551.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16124..17095)
                     /gene="secF"
                     /locus_tag="KLK57_001119"
     CDS             complement(16124..17095)
                     /gene="secF"
                     /locus_tag="KLK57_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HBE0891552.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17106..18953)
                     /gene="secD"
                     /locus_tag="KLK57_001120"
     CDS             complement(17106..18953)
                     /gene="secD"
                     /locus_tag="KLK57_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HBE0891553.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="KLK57_001121"
     CDS             complement(18981..19313)
                     /gene="yajC"
                     /locus_tag="KLK57_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HBE0891554.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="KLK57_001122"
     CDS             complement(19336..20463)
                     /gene="tgt"
                     /locus_tag="KLK57_001122"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HBE0891555.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20519..21589)
                     /gene="queA"
                     /locus_tag="KLK57_001123"
     CDS             complement(20519..21589)
                     /gene="queA"
                     /locus_tag="KLK57_001123"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HBE0891556.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21682..22263
                     /gene="acpH"
                     /locus_tag="KLK57_001124"
     CDS             21682..22263
                     /gene="acpH"
                     /locus_tag="KLK57_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HBE0891557.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="KLK57_001125"
     CDS             complement(22268..24085)
                     /gene="malZ"
                     /locus_tag="KLK57_001125"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HBE0891558.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKHRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLGLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGSTEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKERHGQLTDGILALPAISATVWMN"
     gene            complement(24241..25614)
                     /gene="proY"
                     /locus_tag="KLK57_001126"
     CDS             complement(24241..25614)
                     /gene="proY"
                     /locus_tag="KLK57_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HBE0891559.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRCLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="KLK57_001127"
     CDS             complement(25690..27009)
                     /gene="brnQ"
                     /locus_tag="KLK57_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HBE0891560.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="KLK57_001128"
     CDS             complement(27416..28711)
                     /gene="phoR"
                     /locus_tag="KLK57_001128"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HBE0891561.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEESGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="KLK57_001129"
     CDS             complement(28769..29458)
                     /gene="phoB"
                     /locus_tag="KLK57_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HBE0891562.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDCAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29648..30850
                     /gene="sbcD"
                     /locus_tag="KLK57_001130"
     CDS             29648..30850
                     /gene="sbcD"
                     /locus_tag="KLK57_001130"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HBE0891563.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETTQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30847..33990
                     /gene="sbcC"
                     /locus_tag="KLK57_001131"
     CDS             30847..33990
                     /gene="sbcC"
                     /locus_tag="KLK57_001131"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HBE0891564.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVASALAQHSEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTTSLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALTGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLLQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34116..35300
                     /gene="araJ"
                     /locus_tag="KLK57_001132"
     CDS             34116..35300
                     /gene="araJ"
                     /locus_tag="KLK57_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HBE0891565.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISVFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKP
                     LG"
     gene            complement(35443..36351)
                     /gene="mak"
                     /locus_tag="KLK57_001133"
     CDS             complement(35443..36351)
                     /gene="mak"
                     /locus_tag="KLK57_001133"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HBE0891566.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVALNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36476..37387
                     /gene="rdgC"
                     /locus_tag="KLK57_001134"
     CDS             36476..37387
                     /gene="rdgC"
                     /locus_tag="KLK57_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HBE0891567.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFALRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37465..37920)
                     /locus_tag="KLK57_001135"
     CDS             complement(37465..37920)
                     /locus_tag="KLK57_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891568.1"
                     /translation="MTQHPWSKLQREIYDLLTPTINLQIHCTRYLMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(37917..38252)
                     /locus_tag="KLK57_001136"
                     /pseudo
     CDS             complement(37917..38252)
                     /locus_tag="KLK57_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
BASE COUNT         9474 a        10314 c         9727 g         8822 t
ORIGIN      
        1 tgtttaccca acatgcgctc cgcactacac tatgtgggca tttttttaag gggtggtaat
       61 gaacgattat aaaatgacgc caggtgagag gcgcgcgacc tggggtttag ggaccgtatt
      121 ctcgttgcgc atgctgggca tgtttatggt tctgccggtt ctgaccacgt acggcatggc
      181 tctgcaaggt gccagcgaag cattaatcgg tattgccatt ggtatttatg gtctgactca
      241 ggccgttttt cagattccgt ttggcctgct ttccgaccgc attggtcgca aaccattaat
      301 tgtcggtggg ctggcggtgt ttgccgccgg tagcgttatc gctgcgcttt ccgactccat
      361 ctggggaatt attctgggcc gggcgctaca aggctctgga gcaattgccg ccgccgttat
      421 ggcgctgctt tccgatctca cgcgcgaaca aaaccgcacc aaagcgatgg cgtttatcgg
      481 cgtgagcttt ggcattacct ttgccattgc gatggtgctt ggcccgatca tcactcacaa
      541 acttgggctg cacgcgctgt tctggatgat cgctattctg gcaacaaccg gcattgcgtt
      601 gaccatttgg gttgtgccca acagtagcac tcacgtactt aatcgtgagt ccggaatggt
      661 gaaaggcagt ttcagtaaag tgctggcgga accgcggctg ctgaaactca actttggcat
      721 tatgtgtctg cacatgcttc tgatgtctac gtttgttgcc ctgcccggac agctagctga
      781 tgcggggttc ccggcggctg aacactggaa ggtctatctg gcgacgatgt taatcgcctt
      841 tggctcgatc gtgcctttca ttatctacgc tgaagttaag cgcaaaatga agcaagtctt
      901 tgtcttctgc gtcgggttga tcgtggttgc ggaaattgtg ttgtggaacg cacaaacgca
      961 gttctggcaa ctggtggtcg gcgtgcagct tttctttgtg gcgtttaatt tgatggaagc
     1021 ccttctgccc tcacttatca gtaaagagtc gccagcaggt tacaaaggta cggcgatggg
     1081 tgtctactcc accagccagt ttcttggcgt ggcgattggc ggttcgctgg gcggctggat
     1141 tgacggcatg tttgacggtc agggggtatt tctcgctggc gcaatgctgg ccgcagtgtg
     1201 gctggcagtc gccagtacca tgaaagaacc gccgtatgtc agcagtttgc gcattgaaat
     1261 cccggcggac attgccgcaa acgaagcgtt aaaagtgcgt ttgctggaaa ctgaaggcgt
     1321 caaagaagtg ttgattgcag aagaagagca ttcagcttat gtgaaaatcg acagcaaagt
     1381 gacaaatcgc tttgaggttg aacaggcaat tcgtcaggca taaaaaacgc ggcgaattgc
     1441 ctgctgcgct acgcttacca ggcctacaaa gttcttgagt tacaaaccgt tgtaggccga
     1501 atcaggcgtt catgccgcat ctgacatgaa caaaacgcac attgtcgcga ttaatcgcgg
     1561 aagtttttga actggaacgg ctggccgaga tcgccaccac gtaccatcgc catgacagcc
     1621 tgcaaatcat cacgagattt gccagtcaca cggatctcat cgccctgaat ttgcgcctgc
     1681 actttcagtt tgctgtcttt aatcatcttg acgattttct tctgggtcgc gctctcaatg
     1741 ccctgtttca gtttcgcttc cacaaaccag gttttaccgc tatgaacgat attttccggt
     1801 acatccagtg aactgccttc aatgccgcgc ttcagcagct tggcacgcag aatatccagc
     1861 aactgattga cctggaaatc ggactcgctc aacactttga tggttttgct ggcgtcgttc
     1921 agctcaaatg aggcttcaac attacggaag tcaaaacggg actccacttc gcggctcgcg
     1981 ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag agacaatatc gaaagatggc
     2041 atcttttcct ctcccttcat ttttgatgcg aagcataata cccgcaaagt taaataaccg
     2101 acagtcctgc accaggacgt atcccttaat tgtgcatagt cacaacagca tgcacaatta
     2161 agggatagcc ttattgaaca cccggagtgg ttgcgggtga ggaggaacaa tgaaaattac
     2221 cgtattggga tgcggtgcct tagggcaatt atggcttaca gcactttgta aacagggtca
     2281 cgaagttcag ggctggctgc gcgtaccgca accttattgt agtgtgaatc tggttgagac
     2341 tgatggttcg atatttaacg aatcgctgac cgccaacgat cccgattttc ttgccaccag
     2401 cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc gatgccgtca aaagcctcgc
     2461 gtccacactg cctgtaacca cgccaatact gttaattcac aacggcatgg gcaccatcga
     2521 agagttgcaa aacattcagc agccattact gatgggcacc accacccatg ccgcccgccg
     2581 cgacggcaat gtcattattc atgtggcaaa cggaatcacg catattggtc cggcacggca
     2641 acaggacggc gattacagct atctggcgga tattttgcaa accgtgttgc ctgacgttgc
     2701 ctggcataac aatattcgcg ccgagctgtg gcgcaagctg gccgtcaact gtgtgattaa
     2761 tccactgacc gccatctgga attgcccgaa tggtgaatta cgtcatcatc cgcaagaaat
     2821 tatgcagata tgcgaagaag tcgcggcagt gatcgagcgc gaagggcatc atacttcagc
     2881 agaagatttg cgtgattacg tgatgcaggt gattgatgcc acagcggaaa atatctcgtc
     2941 gatgttgcag gatatccgcg cgctgcgcca caccgaaatc gactatatca atggttttct
     3001 cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa aacacccgcc tgtttgaaat
     3061 ggtaaaaaga aaggagagtg aatatgagcg catcggcact ggtttgcctc gcccctggta
     3121 gtgaagagac tgaagccgtc accactatcg atctgctggt tcgcggcggt atcaaagtca
     3181 ccactgccag cgtcgccagc gatggcaacc tggccattac ctgctcgcgc ggcgtgaagc
     3241 tgctggcgga tgcgccgctg gtcgaggtgg ctgatggcga atatgacgtg atcgtactgc
     3301 ctggtggcat taaaggcgca gagtgttttc gcgatagtac cctgctggtt gaaaccgtta
     3361 aacagttcca ccgttccggg cgtatcgtcg cggctatttg cgccgcgcca gccaccgtgc
     3421 tggtaccgca cgatatcttc ccgattggca atatgaccgg cttcccgacg ctgaaagaca
     3481 aaattcccgc cgaacaatgg caggacaagc gcgtcgtctg ggatgcacgg gtaaaattgc
     3541 tgaccagcca ggggccgggt acggctattg attttggtct gaaaattatc gacctgctgg
     3601 ttgggcgcga gaaaacccat gaagtggcat cacaactggt gatggcggca gggatttata
     3661 attattacga gtagtgtcgg atgcggcaaa cgtcgcatct gaccagatgc gacgtaaaaa
     3721 ccacaaatta tgggcgatac accttcacat tgttaaagcc ctgctcgcgc agatagagcg
     3781 cctgcagacg gctcatcacc ccgcgctcac accacagtag ccaggttctg ttctggtcga
     3841 gatcgccaaa tttggtgctc agtttataga acggcagaga aaccacgtcg ataccttcaa
     3901 ccttcagcgg cttatcttcc tgctcatcga cagaacggat atcgaggatc acgtcgttcg
     3961 ggccaaagcc attgacggtt tccacttcca ccacttcctg ctcggtctgc tgggcgattt
     4021 cgcggatatc aacgttattc gcttcctcaa ccactttatc gagaatactg aagtcgaact
     4081 tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac cgtcgggctt ttggagatca
     4141 cgccacaata ttctggcatc gtgcgagcaa agtcttcagt gccaatctgg cgggccaggt
     4201 tgatgatgtg ctctttgtcg taagaaatca gcggacgcag gatcagcgta tcagagacgt
     4261 tatcaatcag gcgcaggttg gtcagcgtct gactggacac ctggccgagc gcttcgccgg
     4321 tgaccagcgc ctgtacgccg taacgttcag ccactttaga cgcggcacgc accatcatac
     4381 gtttgaggat aacgcccatc tgaccgtcgt cgattttctc gagaatttcc ccgacgaccg
     4441 gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct gccaaagcgg ttccacagat
     4501 aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc gccgaggtta aagaagcagt
     4561 aatgcacgcg gcagccgcga cgcatcaaca tataactgga aacaccggag tcgaaaccac
     4621 cggaaatgag cgacagcaca tcttcctggg tgccaatcgg gaaaccgcca ataccttcgt
     4681 agcggccttt aatcagcaga agacgatcgt cttccacttc cagatggaca gttacatccg
     4741 ggttagtcag cttcacgcgc gcggattcaa tatgctgatt taaaccgccg ccgacgtaac
     4801 gttccacatc aatcgagcta aaatcatgtt tgccacggcg cttcacgcgt acgcagaagg
     4861 ttttgccttc cagctgatcg cgatattgaa ccaacgcttt ctcgaaaata tcgtgcatgt
     4921 cggtaaacgg cacgtcttcg acttcgagaa tatggtggat acccggaata cgggtcaggg
     4981 cgtcgcgaat agccagacgc tgtttttcat cttttgcgcg aacttcgatg ttatcccagt
     5041 ggcggacgac agcgagcgtc tcatcatagt gctttaaaac gttacgaatg ttcccggtaa
     5101 ggatttttat aaagcgcaag cgcacagatt ggcttttgat ggtgatttcc gggaacaatt
     5161 taatgataaa cttcatggcg gcaatggttc gttggcaagc cttaagcgac ttgtataggg
     5221 aaaaatacag cagcccacac ctgcggctgc atccaggcgc ggaagtatac cactaacatc
     5281 gctttgctgc gcacatcacc ttaccattgc gcgttatttg ctatttgccc tgagtccgtt
     5341 accatgacgg ggcgaaaaat attgagagtc agacattcat tatgccgaag aaaaatgagg
     5401 cgcccgccag ctttgaaaag gcgctgagcg agctggaaca gattgtaacc cgtctggaaa
     5461 gtggcgacct gccgctggaa gaggcgctga acgagttcga acgcggcgtg cagctggcac
     5521 gtcaggggca ggccaaatta caacaagccg aacagcgcgt acaaattctg ctgtctgaca
     5581 atgaagacac ctctctaccc ccttttacac cggacaatga gtaatggact ttccgcagca
     5641 acttgaagcc tgcgttaagc aggccaacca ggcgctgagc cgttttatcg ccccactgcc
     5701 ctttcagaac actcccgtgg tcgaaaccat gcagtatggc gcattattag gtggtaagcg
     5761 cctgcgacct tttctggttt atgccaccgg tcatatgttc ggcgttagca caaacacgct
     5821 ggacgcaccc gctgccgccg ttgagtgtat ccacgcttac tcattaattc atgatgattt
     5881 accggcgatg gatgatgacg atctgcgtcg cggtttgccg acctgccatg tgaagtttgg
     5941 cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg ctggcgttct cgattttaag
     6001 cgatgccgat atgccggaag tgtcggaccg cgacagaatt tcgatgattt cagaactggc
     6061 aagcgccagc ggtattgccg gaatgtgcgg tggtcaggca ttagatttag atgcggaagg
     6121 caaacacgta cctctggacg cactcgagcg tattcatcgt cataaaaccg gcgcattgat
     6181 tcgcgccgcc gttcgccttg gtgcattaag cgccggagat aaagggcgtc gtgctctgcc
     6241 agtactcgac aagtacgcag agagcatcgg ccttgccttc caggttcagg atgacatcct
     6301 ggatgtggtg ggagatactg caacgttggg aaaacgccag ggtgccgacc agcaacttgg
     6361 taaaagtacc taccctgcac ttctgggtct tgagcaagcc cggaagaaag cccgggatct
     6421 gatcgacgat gcccgccagt cgctgaaaca actggctgaa caatcactcg atacctcggc
     6481 actggaagcg ctagcggact acatcatcca gcgtaataaa taaacaataa gtattaatag
     6541 gcccctgatg agttttgata ttgccaaata cccgaccctg gcactggtcg actccaccca
     6601 ggagttacga ctgttgccga aagagagttt accgaaactc tgcgacgaac tgcgccgcta
     6661 tttactcgac agcgtgagcc gttccagcgg gcacttcgcc tccgggctgg gcacggtcga
     6721 actgaccgtg gcgctgcact atgtctacaa caccccgttt gaccaattga tttgggatgt
     6781 ggggcatcag gcttatccgc ataaaatttt gaccggacgc cgcgacaaaa tcggcaccat
     6841 ccgtcagaaa ggcggtctgc atccgttccc gtggcgcggc gaaagcgaat atgacgtatt
     6901 aagcgtcggg cattcatcaa cctccatcag tgccggaatt ggtattgcgg ttactgccga
     6961 gaaagaaggc aaaaatcgcc gcaccgtctg tgtcattggc gatggcgcga ttaccgctgg
     7021 catggcgttt gaagcgatga atcacgcggg cgatatccgt cctgatatgc tggtggtcct
     7081 caacgacaat gaaatgtcga tttccgaaaa tgtcggcgcg ctcaataacc atctggcaca
     7141 gctgctttcc ggtaagcttt actcttcgct gcgcgaaggc gggaaaaaag ttttctctgg
     7201 cgttccgcca attaaagagc tgctcaaacg taccgaagaa catattaaag gcatggtagt
     7261 gcctggcacg ttgtttgaag agctgggctt taactacatc ggcccggttg acggtcacga
     7321 tgtgctgggg cttatcacca cgctgaagaa catgcgcgac ctgaaaggcc cgcagttcct
     7381 gcatatcatg accaaaaaag gtcgtggtta tgaaccggca gaaaaagacc ccatcacttt
     7441 ccacgccgtg cctaaatttg atccctccag cggttgtttg ccgaaaagta gcggcggttt
     7501 gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa acggcagcga aagacaacaa
     7561 gctgatggcg attactccgg cgatgcgtga aggttccggc atggtcgagt tttcacgtaa
     7621 attcccggat cgttacttcg acgtggcaat cgccgagcaa cacgcggtga cctttgccgc
     7681 cggtctggcg attggtgggt acaaacccat tgtcgcgatt tactccactt tcctgcaacg
     7741 cgcctatgat caggtgctgc atgacgtggc gattcaaaag ctcccggtcc tgttcgccat
     7801 cgaccgcgcg ggcattgttg gtgctgacgg tcaaacccat cagggcgctt ttgacctctc
     7861 ttacctgcgc tgcataccgg aaatggtcat tatgaccccg agcgatgaaa acgaatgtcg
     7921 ccagatgctc tataccggct atcactataa cgacggcccg tccgcggtgc gctacccgcg
     7981 cggtaacgcg gttggcgtgg aactgacgcc gctggaaaaa ctgccaattg gcaaaggcat
     8041 tgtgaagcgt cgtggcgaga aactggcgat ccttaacttt ggtacgctga tgccagacgc
     8101 ggcgaaagtc gctgaatcgc tgaacgctac gctggtcgat atgcgttttg tgaaaccgct
     8161 tgatgaagcg ttaattctgg aaatggccgc cagccatgaa gcgctggtca ccgtagaaga
     8221 aaacgccatt atgggcggcg caggcagcgg cgtgaacgaa gtgctaatgg cccatcgtaa
     8281 accagtaccc gtgctgaaca ttggcctgcc tgacttcttt attccacaag gaactcagga
     8341 agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg gaagccaaaa tcaaggcctg
     8401 gctggcataa tccctactcc actcctgcta tgcttaagaa attattcatg gcaggagtgg
     8461 cagcatgcaa tacaacccct taggaaaaac cgaccttcgc gtttcccgac tttgcctcgg
     8521 ctgtatgacc tttggcgagc cagatcgtgg taatcacgca tggacactac cggaagagag
     8581 tagccgtccc atcattaaac gcgcgctgga aggcggcata aatttctttg ataccgccaa
     8641 tagctattcc gatggcagca gcgaagagat cgttggtcgc gcactgcggg atttcgcccg
     8701 tcgtgaagac gtggtcgttg cgacgaaagt gttccatcgc gttggtgatt taccggaagg
     8761 attatcccgt gcacaaattt tgcgctctat caacgacagc ctgcgccgtc tcggcatgga
     8821 ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac acgccgatcg aagagacgct
     8881 ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt tatatcggcg cgtcatccat
     8941 gcacgcttcg cagtttgctc aggccctgaa gctacaaaaa cagcacggct gggcgcagtt
     9001 tatcagtatg caggatcact acaatctgat ttatcgcgaa gaagagcgcg agatgctgcc
     9061 tctgtgttat caggagggcg tggcggtgat tccgtggagc ccgctggcgc gggggcgact
     9121 gacgcgtccg tggggagaaa ctaccgcacg actggtgtct gatgaggtgg ggaaaaatct
     9181 ctataaagaa agcgatgaaa atgacgcgca gatcgcagag cggttaacgg gcgtcagtga
     9241 agaactcggt gcaacacgag cacaagttgc gctggcctgg ttattgagta aaccgggcat
     9301 tgccgcaccg attatcggta catcgcggga agaacagctt gatgagctgc tgaacgcggt
     9361 ggatatcact ttgaagccag aacagattgc cgaactggaa acgccgtata aaccgcatgc
     9421 ggtcgtagga tttaaataag agtccaggcc tgatgagacg tgacaagcgt cacatcaggc
     9481 atcggtgcac aactacgaca gaatacccag cggccagtga tgaccgataa agtacaggat
     9541 gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg atccccatgc cgccatgtac
     9601 attgcgatca aaccagcgga tcggccacgg cttccacata tccagaatac ggaaaatcac
     9661 aaacccggcg gcaacccact gccagtcatt ggtcggcagc gccatgagcg tgatccacat
     9721 accaataaat tcgtcccaga caatgctgcc gtgatcgtgc acgcccatat ctttcgccgt
     9781 ttgatgacaa agatagacgc cgatacagat ccccaacatc accaccagcg agtagagctg
     9841 ccagggtaaa aaggtcatca gataccagaa cggaatcgct gccagcgatc ccatcgtgcc
     9901 aggaacgatt gggcttaatc cactcccgaa tccgacagca agcagatgcc acggattact
     9961 catcttcagg cgacttttcg cgacatcttt atggcgtggc aaaatggtca tatcctttcc
    10021 agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag cccttcgata tcggcggtca
    10081 tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag agcaacatcc agcgcgccac
    10141 ggttcagttc cggcaccgtg aaacacaact cgtaatcttc accgccagag agcgcccagc
    10201 gcagcgcctg ttccggttca acatggcgag aaagcgcatc agaaaacggc agcaatgcca
    10261 ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg cccgagatcg gaaatcagac
    10321 cgtcagagag atcgatagct gaatttgcca gatcgcgcag tgcctgcccc tgtaaaatac
    10381 gcggcgatgg acggagatga cgtttgatca agtagtcagc atctttagca tcggcaacct
    10441 gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc gcccggtgta ccggtcacat
    10501 agatccagtc acccggtttc gccccagagc gcgttaaggc tcgtcccatc ggaacaaagc
    10561 cgtggatacc caacgtcatt gataatggcc cacgcgtggt atcgccgcca atgagttgca
    10621 tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa ggactcaagc cacgcttcgt
    10681 ctacgtccgg taaggttaat gccagcgtca gccaggccgg atcggccccc atcgctgcca
    10741 gatcgcttag gttcaccgcc agtgctttat aagccagatc agcaggatcg atatcaggga
    10801 ggaaatggtt acccgccacc agcgtatcag tgctgatcgc cagggtctgt ttctcgggga
    10861 tattgagaag tgcgcaatcg tcgccgatgc ccagttcgac atcaagacga gaacttctta
    10921 cacggtcaaa ataacgggca atcagggaga actcgccaca tgccatacgt tatgcctcag
    10981 caggtaaaaa agaaaaggcc gacgatcatg gaacggtctt ccgtgaatct accggcctgg
    11041 atatcacttt ttgttagggc gaatcacagg tgctgcttta tcgagtacgc cgttgacgaa
    11101 cttatggctg tcttctgcgc cgaacgattt cgccagttcg atcgcttcgt taatggccac
    11161 tttgtatggc acatcgctac gtttagacag ttcgtacagc gcaatgcgca gtactgcttt
    11221 ttctacctgt cccagttctt ccagcaggcg ggacaggtat ggcttcatca gtccgtcgag
    11281 gtatgcggta ttagtcgcca ccccggccag cagctcacgg aagtacagga cgtcaacgtc
    11341 ttttacatcc tgttcagcca ggaactggta ttcaacatca gcgatgtcgt tctgggacaa
    11401 ctgccaggag tagagcgcct ggacggcaca ctcacgagcg cggcgacgag cagcaggttt
    11461 cacggatttc cccttactaa tttcaggcct tgatggcttt caatacatta atcatttcaa
    11521 gcgcggtcag tgcagcttct gcacctttgt tgccagcttt ggtgccagca cgttcgatcg
    11581 cttgttcaat gctttcagtg gtcagaaccc caaaagcaac cggaatttcg ctgtcctggg
    11641 caacatgcgc caggccgttg cttgcaccac cagcgacata ttcaaagtgg gcagtgccac
    11701 cacgaataac cgtacccagc gcaatcaccg cgtcgtattt accggtttta gccagtgcac
    11761 ccgccgccag cggcagctca taggcaccag gcacccaaac aacggtaatg ttttcatctt
    11821 ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc cagcaggctg tcattgataa
    11881 agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc cggggtagca acgttagctt
    11941 caataatgtt cattttcttc cttcgggttc gagtatggcc ccgcaggggg gcggatttta
    12001 gcataatatt tcgtgcgctg cttccctttc gagccgggag atcatgcacc cactaaatgc
    12061 aggcaaacat ccgggcctac atgacgtatc tctttgaatt taaattgggg ggcgtcggct
    12121 aatttctcaa gccctggcag cgtgcataat ccgcgggcgt cgctgcctaa tagtttaggt
    12181 gcgatataga caatcagctc atcgactaaa cccgcctgca gcaatgcgcc agcgagcgtt
    12241 ggccccgctt ccacccagat gctgttaatt tgctgtttac ccagttgcat catcagtaca
    12301 accagatcca gatgaccttt atgctctgga atcagcaagg tacgcaccgt ttccggccac
    12361 tcactagaat cttcctgcgt acgcgcgaac caggtttcgc cgggctgctg cacaatgcga
    12421 tgttccggcg tcacgcgatt ttggctatca atcacaatac gtactggctg gcggagattt
    12481 tgttgcgggt agagcgcctg agtttgttca tccagttcag accaacgcac tgttaaggct
    12541 ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg catgactttg tgcgcgctgt
    12601 cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact ggctttcgcc gctcgccatc
    12661 gccgtgcggc catcaagcga tgcgccaagt tttaactgaa tataaggaaa gccggtgcgc
    12721 atccgcttga gaaagccttt attcaattgc tcggcttcac tcatcatcag cccgtggctg
    12781 acgtcaatgc cagcctgttg cagacggtaa agtccacgcc cagcgacctg cgggttagga
    12841 tcctgcatcg ctgcgaccac gcgcgctacg ccagcggcga tgagtgcgtc acagcacggt
    12901 ggcgtacggc catgatggct acagggttcg agtgtcacat acgccgttgc gcctttggct
    12961 ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg gttcacccgc tcgttggtgg
    13021 taaccttcac cgacaatttc gccatctttg acaatgacgc ccccgacatt cgggttgggg
    13081 tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc gcgccatgta atactcgtcc
    13141 tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa attctttgat atcttcgaaa
    13201 ctgcggtaga cagaggcaaa acggatatag gcgactttat cgagcttttt caattgctcc
    13261 atcaccagat tgccaatcat cttgctcggt acttcgcgct caccggtggc gcgcagctgc
    13321 gatttaatat gattgattgc catttcaacg tcatcggaac tcaccggacg tttttccagc
    13381 gcccgcagca ttccgctacg caatttctct tcattaaacg gttcacgcac gtcgttgctt
    13441 tttacaacac gcggcataac cagctccgcc acttcaaagg tggtgaaacg ttcattacac
    13501 accagacact gccgacggcg gcgtacggat gaaccctcgc ccacgagacg agagtcaatt
    13561 accttagtgt ccacggcgaa acagaatggg caatgcatac ggcgtcctga ccaggtggtt
    13621 aacggaaatc tattttaccc tgaactgacg atacaacaaa ggcgcaacgc ttttgagata
    13681 aaggatcgat gctttcgctg cttttaccct ctaccattag gctaattgcg acgagaaagg
    13741 gacgacctat gaatactaac gtttttcgac tgctcctgct gggaagcctg ttcagcctta
    13801 gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca cagcgtcagc acgctgaacc
    13861 aggagatgac gcaactcaat caagaaacag tcaaaatcac tcagcaaaac aggctgaatg
    13921 caaaatccag cagtggggtt taccttctgc ctggagcgaa aacaccggca agactggaaa
    13981 gccagatcgg tactttacgt atgtcgctgg tgaatattac gcctgataca gatggcacca
    14041 cccttacact acgtattcag ggtgagtcga atgatccctt gcccgcattc agcggaactg
    14101 ttgaatatgg gcaaattcag ggaacaatag acaactttca ggaaatcaat gtgcagaatc
    14161 aattgattaa tgcccctgcc agcgtcctcg cccccagcga tgttgatatt ccgttacaat
    14221 taaaggggat ttctgtggat cagttaggct ttgtacgtat ccacgacatt caacctgtta
    14281 tgcagtaaac gtatttcggg acgattttgt gcgtcccgca acatctttcc ccgtcatttt
    14341 gttactctgc ttacatcacc tggattgata gtaaaagttt gcaacaaggg cgaaagtcag
    14401 tacaatcccc gcccgaatgt gtgtaaacgt gaacgcaatc gattacgtaa atgatagaac
    14461 tgtgaaacga aacatatttt tgtgagcaat gatttttata ataggctcct ctgtatacga
    14521 aatatttaga aacgcaattt gcgccttttt cactcccgca agggattttc aaacagtggc
    14581 atacatatga aaaaaacatt actggcagcc ggtgcggtac tggcgctctc ttcgtctttt
    14641 actgtcaacg cagctgaaaa cgacaaaccg cagtatcttt ccgactggtg gcaccagagc
    14701 gttaacgttg tcggaagcta tcacacccgt ttcggaccgc agatccgcaa cgatacctac
    14761 cttgagtacg aagcattcgc taaaaaagac tggttcgact tctatggtta tgcggatgcg
    14821 ccggtattct tcggcggtaa ctccgatgct aaaggtatct ggaaccacgg ttctccgctg
    14881 tttatggaaa tcgaaccacg tttctccatc gacaagctga ccaatactga ccttagcttc
    14941 ggtccgttca aagagtggta cttcgcgaac aactacattt acgacatggg tcgtaataaa
    15001 gatggtcgcc agagcacctg gtacatgggt ctgggtaccg acatcgatac tggtctgccg
    15061 atgagcctgt ccatgaacgt ctatgcgaaa taccagtggc agaactatga cgcagcgaac
    15121 gaaaacgagt gggacggtta ccgtttcaaa attaaatact ttgtgccgat taccgatctg
    15181 tggggcggtc agctgagcta catcggcttc accaacttcg actggggttc cgatttaggg
    15241 gatgacagcg gtaacgcaat caacggtatt aagacccgta ctaataactc tatcgcttcc
    15301 agccatattc tggctctgaa ctacgatcac tggcactact ctgtcgtagc tcgttactgg
    15361 cacgacggtg gtcagtggaa cgacgatgca gaactgaact tcggcaacgg caacttcaac
    15421 gttcgctcta ccggctgggg tggttacctg gtagtaggtt acaacttctg attatgaaaa
    15481 tgccgggatt tattcccggc atttctgatt gttaacaacg tgtgctttgt tcatgccgga
    15541 tgcggcgtaa acgctttgtc cggcccacaa aagcacgtaa attcaatata ttgctgaaat
    15601 aacgtaggcc tgataagcgt agcgcatcag gcaattttac gttttaatca cttcttcttg
    15661 ttcatcatcg ctttcaggtc agcgaatggg ttgtacttcg cttcaccgac atctttctgc
    15721 gcatcttccc ctgcgataac ggtcgtacca tactgatccg cttcggtata tttcgaatgc
    15781 tcatgatcgt ggcaatagag acaaagcaat tcccagttac tgccatcttc cgggttattg
    15841 gtatggtcgt gatcaatgtg gtgaacggta agttcacgca ggttggaata aacaaactcg
    15901 cgggaacagc gaccgcagac ccacggatag atttttaatg ctttttcgcg atagccgctt
    15961 tctaaccgcg catagttttt tgggatgata gccatgaaaa gttacctgga tgaaatgtag
    16021 ggttacgcgc agtttcccaa gatgcgggga ttttcgcaat ctccatacat caaaaaaaat
    16081 cccgatcttc tgaccgggat tttcaacatc aacgggaact tgattacggc agaattgacg
    16141 gctgatccgc cccttctttt tccactttct gctgcaacat gtgttcgcgc ttcataccca
    16201 gtttcagagc caacgcagat gctacgtaga tggaagatgc agtgccgatg gaaacaccga
    16261 taagcatggt cagcgagaag ccttccagta ccggaccacc gaagagatac agcatcagga
    16321 taaccatcaa ggtagtaccg gatgtgatca aggtacggtg cagcgtctgg gtcaaggaca
    16381 cgttaaagat ttcgtaaggc gtaccgcgac ggatcttgcg gaagttttca cgaattcggt
    16441 ccgataccac gatactgtcg ttaagcgagt aaccgataac cgacatcaac gatgccacaa
    16501 tggtcaggtc aatctcgata tggaataacg acaaaatacc cagcgtaatg ataacgtcgt
    16561 gcgccagcgc aataaccacc cctgccgcca gtcgccactc aaagcggaaa cctacgtaca
    16621 cgaggataga cagcagcgct gccatcaacg ccatcgcacc ggtttgcgca aggtctgccc
    16681 ccacgctcgg accgacgaac tcaatacgct tcactgctgc attctgattg gtggattcgt
    16741 taatcacctt cagaacctgg ctgcccagca cctgaccgcc ggtttcgcct tcagcaggcg
    16801 gcatacggac catgatgtcg tggctgctac caaagttttg cagcattggc tcttcaaaac
    16861 cggctttttg caatgcatca cgcattacgt caatttcagc cggtttttcg agcgtaattt
    16921 cgataaccgt accaccggtg aaatccagcc cccagttaaa gccgcgcacg cccataataa
    16981 cgatagcagc gattaacagc agaccagaga tgccgaaagc ccagtagtcc cagcgcataa
    17041 agtcatagac tttacggccg tggtttagtt gttcaacagt atattcctgt gccacatcgc
    17101 actcctcaga ttgacagctt cttgacgcgc ttgccgccat atagcaggtt tacgatggca
    17161 cgcgtaccga caatcgcggt aaacatcgac gtcgccacac cgataccggt agtaatcgcg
    17221 aaccctttaa ttgccccggt acccactgcg tacaggatga tgactttaat cagcgtggtg
    17281 atgttcgcat cgaagataga actgaatgcg ccacgataac cttcatcaat tgcctgttga
    17341 acagtacgtc cgttgctcaa ctcttcttta atacgttcgt tgatcagtac gttcgcatcg
    17401 accgctaccg caagggttaa gacgataccc gcgatacctg gcatactcag tgttgcgcct
    17461 ggcaacagcg acataatacc gacgatcaag atcaagttgg caatcagagc actggtcgca
    17521 atcagaccaa acttcttata gaagatgatc atgaacagaa tagaaaccag cagaccggca
    17581 aggcaagcct ccagcccctg ctcaatgttc tgcataccca gggtcgggcc aattgtacgt
    17641 tcttcaacaa tctgaatcgg cgcgatcaac gcaccggcac gcagcagcag tgacagctga
    17701 cgggcttcgt tcgggttgtt gatgccggtg atacggaagc tgttacccag acgagactgg
    17761 atgttggcga tgttaatcac ctcttcctgt ttcaccagaa ccgcacgacc atttgcatct
    17821 ttcttaccgc tgtctttgta ctccacaaac agggttgcca tcggtttgcc gatgttgtcc
    17881 ttggtgaagt tagacataat gttgccacca gcgctatcga gcgagatgtt aacctgcggc
    17941 tggttgtatt cgtcctggct ggaagtggag tcggtgatat ggtcaccggt cagaattacg
    18001 cgtttgtaca gcacaaccgg ctgaccttcg cgggtctgtt tcacttcaga gtcgcccggt
    18061 acgcgaccgg atgccgcagc ggcctgatca acattggtgt ttaccagacg gaattccagc
    18121 gttgccgtcg cgcccagaat ctctttcgca cgcgcagtgt cctgaatacc aggcagttca
    18181 acaacgatac ggtcagcacc ctgacgctga accaccggct ccgccacgcc aagttggttt
    18241 acacggttac gcaggatatt aatgttctgc tgcaccgcat attcacgcgc ttcactcaga
    18301 cgagcatcgc tcattaccgc acgcagctgg ttgctgccct ggctgctaat caccaggtcc
    18361 ggatggcgct tgctcagata cgcaatggct tcatcacgag ctttagcatc gcggaaagtg
    18421 atgctcaggc cgtagttgtt ttctttacga acagtggtat acgggatgcc cttttcgcgc
    18481 aggtcactgc gtaggctatc gatattttgt tcctggagtt tgccaagcgc ggtgtccata
    18541 tccacttcca tcaggaagtg aacgccgcca cgcaggtcaa ggccgagctt catcggctca
    18601 gcatgaatag ctgccagcca gcgcggcgtt gccggggcaa ggttaagcgc cacgacgtat
    18661 ttgtcaccca taacgcccat taatgcttca cgagcgcgca actgggtgtc agtggagtcg
    18721 aagcgcgcaa gaatagcgcc ctcttccagt gccacagact tagcagttat tttttcttct
    18781 tgtaacgttt tctggacctg gatcagcgtt tgctcactgg cggcgactcc gcgcgcacca
    18841 gtgatctgaa cagccggatc ctcaccaaac aggttgggaa gcgcatacag cagaccgatg
    18901 acaatcacca cgatcagcat gacgtacttc cacaaaggat aacggtttaa cacggcaatt
    18961 cccttaggga aaaattttaa ttacagcgcc ttcatggtgc ctttcggcag gacggcagct
    19021 acgaagtcac gtttaataac tacttcagtg gtgtcattca gcgcgatagc aatgtagccg
    19081 ttttccgcta ctttggttac gcgaccaacc aggccaccgt tcgtcagaac ttcatcacct
    19141 ttggcaatgg agtccatcag ctttttgtgt tctttggtgc gcttctgctg tggacgcagg
    19201 atcatgaaat agaaaatcag accgaatacc accagcatca aaatcaaaga catcgggcta
    19261 ccttgcgccg gtgcacccgt tgccgctacc gcatcagaaa taaaaaagct cattaaattt
    19321 ccctcattat taatattaat caacgttcaa aggtggtact tctcgcccct gacgctggta
    19381 aaaatcagtt acgaagctct ctaatttacc ctcttcaata gccttgcgta aacccgccat
    19441 caaacgctgg tagtaacgaa ggttatgaat ggtgttgagt cgcgcgccta atatttcgtt
    19501 gcaacggtca agatgatgca agtaagcgcg tgaataattg cgacaggtgt agcaatcaca
    19561 ctcaggatca agtgggccag tatcgctctt atacttcgca ttgcggattt tcaccacgcc
    19621 atcggtcacg aacaaatgac catttcgggc gttgcgggtt ggcattacgc agtcaaacat
    19681 atcgatacca cgacgtacgc cttcaaccag gtcttctggt ttaccaacgc ccatcaggta
    19741 acgcggtttg tctgccggaa tttgcgggca tacatgctcc agaatgcggt gcatatctgc
    19801 tttcggctca cccacagcca gaccgccgac agcgtagcca tcaaaaccga tatctaccag
    19861 acctttaaca gaaatatcac gtaaatcttc gtaaacgctg ccctgaatga taccaaacag
    19921 cgcatttttg tttccgagac tgtcaaaacg ctcacggcta cgcttcgccc aacgcagaga
    19981 catctccatg gagcgttttg cgtaatccca gtcagcagga tacggcgtac actcatcaaa
    20041 gatcatgacg atatccgaac caagatcgta ctgaatctcc attgattttt caggatcgag
    20101 gaaaatcgga tcgccgttga tcgggttacg gaagtgcacg ccctgttcgg tgattttacg
    20161 aatatcgcca aggctgaaga cctggaagcc gccggagtcg gtgaggatcg gccccttcca
    20221 ctgcataaaa tcgtgcagat cgccgtgcag tttcatgatt tcctggcccg ggcgcagcca
    20281 caggtggaag gtgttgccga ggataatttg cgcgccagtg gcttcaactt cttccggcgt
    20341 catccctttt acggtgccgt aggtgccaac aggcataaaa caaggcgttt ccactacgcc
    20401 acgatcaaag accaggcggc cacggcgtgc gcgaccgtcg gtggtgtcca gttcaaattt
    20461 cattttttct ccactacgtc agaaaaacag tccaacgttt taaaccagcg ccacggaatt
    20521 actccccgac gcgctcatta attgcctgcg gattgtacgt gataaacatc gcatcaccgt
    20581 aactaaaaaa gcgatatttc tcttctaccg ctgctttata ggcgttcatg gtgtgttgat
    20641 aaccggcaaa ggccgaaacc agcataatca gcgtcgattc cggcaggtgg aagtttgtca
    20701 ccagcgcatc gaccactttg tactggaagc ccggatagat aaagatttgg gtatcgtcaa
    20761 agaacggttc gataagatcg tttttcgccg cctgagccgc gctttccagc gaacgtactg
    20821 aggtggtgcc aaccgcaatc acccggttac cgcgcgcttt cgccgccagt accgcgtcta
    20881 ccacatcctg cggcacttca gcgtattccg agtgcatgat gtgatcttca atggtgtcga
    20941 cgcgcaccgg ctggaaggtg cccgcaccaa cgtgcaacgt cacaaacgcc atctccacgc
    21001 ctttggcgcg taatttttcc agcaaaggtt cgtcaaaatg cagacctgcg gtcggggctg
    21061 caaccgcgcc cggtttttcg ctataaacgg tttgataaag ttcgcggtca gcgtcttcgt
    21121 ccggacggtc gatatacggc ggcagcggca tatggccgat gctgttgaga atatccagca
    21181 ccgagcgttc atcattaaat tcgacttcaa acagtgcgcc gtggcgcgcg gtcattgttg
    21241 cgttaatact ttcgtcatcg cccagcagca gttctgcgcc aggttttggc gctttcgagg
    21301 cgcgaatatg cgcaagaatg cgtttgtcgt cgagcatccg ttcaaccagc acttcaatct
    21361 tgccgccgct ggctttacgc ccaaacaggc gcgccgggat cacgcgggta ttattaaaaa
    21421 ccagaagatc gccggggttg agcttatcaa gtaaatcggt gaaagtaccg tgcgtcagcg
    21481 cgcccgtcgg cccgtccagc gacagtaaac gacagctact gcgttcaggc atgggatagt
    21541 gggcaatcag ggattcgggc aattcaaagg agaaatcggt aacgcgcatg acactgactc
    21601 agactaaaat aagaggcggg tagtctagtg ccggggcgct ctccctgcaa cctttacccc
    21661 cttttggata gaatataatc gatgaatttt ttagctcacc tgcatttagc ccatctcgcg
    21721 gaaagctcgc tttccggcaa tttactggct gatttcgtac gcggaaatcc tgaagaaagt
    21781 tttccgcccg acgtcgtggc tggcattcat atgcatcgac gtatcgacgt attaaccgac
    21841 aatctgccgg aagtccgcga agcacgggaa tggtttcgta gtgaaactcg ccgcgttgcg
    21901 cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc gcagctgtcg
    21961 ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat gacgattttg
    22021 ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga gcagtggctg
    22081 gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc aagccgccgc
    22141 ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta tgacgccctc
    22201 gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca caaggcgtta
    22261 taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga atcccgtcag
    22321 tcagttgccc atgcctttct ttgcattgcc attgcacggc attgagcaac ggtgacgcgg
    22381 gtagcacgac ttcacaagct tcgccacggt tgattgccac cagtacacgc tgctgattca
    22441 gcacgcggac aaataccacc acgttatctt ccgcatacag cacctgacag ccgccatgac
    22501 gtagcgcctg acttttctta cgcagcgcaa tcattcgctg gtacagcgcg aataacgccg
    22561 tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg tttttgccat
    22621 ccagtcctac ttcatcaccg taataaatgc acggtacacc aggccaggtg aacagccaga
    22681 ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat cgcgcagtat
    22741 cgtggctgtc gagctgatta aacatacgta attgttgttg atgagaaagc cctgcgcggt
    22801 aattatccat ccaggccata caggtttggg catcaatttg ctgcggatcg taagagatat
    22861 cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc atggcggcat
    22921 cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg acaatatacg
    22981 cttccggctg ggtttctttc gccgcttcgg tgctcccagc aacgtgctgc atattattgc
    23041 gcgccccacc cgcctcccct agcatatgca ccacatccag ccgccagccg tccatactcc
    23101 acggcgcttt cagccaatgg cggacaatac tgtcttcccc gcgataaatt tcattcacca
    23161 gactttccga ctgataatcc agcttcggca agctggcata gccaagccag tcgagcgccg
    23221 tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagggttg tgacaagctc
    23281 cccccgtgcc acgattgtgc ctatcaaacc aggcatggga atcgccactg tggttaaaca
    23341 cgccatccag ccccagccgc attcccagct gctgcgtatt gtggcgtaag cgcagcaacg
    23401 ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg tatttatgta
    23461 cgctgggagc tttaaacacc ggattgagat acagcgccgt cacgccaagc tttttcagat
    23521 acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat ccgcccgcct
    23581 gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct gcatgatggt
    23641 aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga tcagggaaga
    23701 tctgataaaa aatctgatcc gcagcccatt gtgggccgat atccggtaca tcgacggcaa
    23761 actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac caacgctggc
    23821 gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg gagagatcaa
    23881 tcgccgcacg ccatgcggtg acgccaggct gctgctgact gcgatgctta tgcatcgata
    23941 ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt gggtcttcgc
    24001 ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat gggggcaccg
    24061 gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat caccttgcca
    24121 tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg acgggggcgg
    24181 ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag aacgcaagga
    24241 ttactggttt tcagccagct gacgatcgtg gcgacgctta aacatccagc caatcaacag
    24301 cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt gataaccaat
    24361 caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta ccccacccgg
    24421 cactttaaat ttcagcgcct taacttcttc tggcggcaaa cagcgacgga aggcaatttg
    24481 cgacagcagg atcataatcc acacccacac cgtggcgaag gttgccagcg aagcgatcac
    24541 caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca gcgcggtagt
    24601 catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga aaattttcgg
    24661 cgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaaga cgtcactgtt
    24721 aattgccgat agcgaagcgg tcagcacaac aaagttaaga atgctagcgg caaaggtaat
    24781 gcccatatgc tggaacgtca gcacgaacgg gctaccggca gtgccaacct gattccacgg
    24841 gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac gcatcggcac
    24901 ggagttaatc gcacgcggta tcgatttctc aggatctttc gcttcaccgg cggtaatccc
    24961 gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatca ccatcccaag
    25021 ccagccgtta ctgaagaagc cgccgttgct ccacagatta tgaataccgg tcggttgccc
    25081 gccgttgcca atcccccaga tgatgatgcc gaaaccagcg acaatcatga tgatgatggt
    25141 ggcgacttta aagaacgaga accagaattc cagctccccg aagaccttca cgctcatcag
    25201 gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg gcaccgtcgg
    25261 gaaccagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga caataaggat
    25321 ttcaaagcag taggtccagc cggtaatgta acctgccagc gggccgaggt tttcctgcgc
    25381 ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca gcgcacgcat
    25441 aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg gaccggccat
    25501 tttgatggcg tctgccgaac cgtaaaacag cccggtacca attgctgaac ccagtgccat
    25561 aaagcgtatg tggcgggtac ttagcccacg ctttagcttg ttcttacttt ccatcaattc
    25621 ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt aaaacaaatg
    25681 ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat cccagataat
    25741 ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt cggccagcgg
    25801 taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga tcccgtcgag
    25861 aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg aattatgcca
    25921 ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa tagcggtcag
    25981 caccggtacg gagatctgga tcagctggct taagccgagg ttagaaacca ccatcgagaa
    26041 gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg caaagaattc
    26101 tgcacaagca caggtcaggc caactgccgt taccaagcag gcgatgaaga ttaacgccgc
    26161 cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca gaatagcagc
    26221 gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacgga acagcgccag
    26281 gtacagcaga gtcagaccaa cacccgccat caggccagcc cagacggtat aacgggtcag
    26341 cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga taccaaacac
    26401 cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag aaaacgcagc
    26461 gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa tcgcggcaac
    26521 agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca cggtatccag
    26581 cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca ggctgtagat
    26641 aaacagcggc agcgcggaat caccagtcag cggcgcaatc cccacttcaa aggaaacggt
    26701 agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg ttgccagcag
    26761 cacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc caacttttgc
    26821 cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc cgaatgccgc
    26881 agtccagacg tgttcgccag cctgcaagcc gaccattgga gggaaaataa tgttacctgc
    26941 gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg atcttaattg
    27001 atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt tttgtgccaa
    27061 tccctaggga tgatacagcg gtatatttaa acaattttag cgggaaatac tcccaaccag
    27121 gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg acagcacctg
    27181 aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa accagaggta
    27241 tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa agacatggct
    27301 aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat tttgactgca
    27361 aagaaaataa gccagcatcg actggcttat ggcaataaaa gatgacaaag gcggattaat
    27421 cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt ccttttccta
    27481 ctgtactctc aatattcagg cgactttcgt gatgattcac agcatgtttc acgatcgcta
    27541 accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga taaaaacgct
    27601 cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca acgctaaatt
    27661 cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttct ggagtatgat
    27721 tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg ttgccagaca
    27781 ctttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc agagtctgag
    27841 cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg agcaattgcg
    27901 tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct tccatccgct
    27961 gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc agcggctgct
    28021 catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac tcatggctca
    28081 cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg acatcacgcg
    28141 ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga tgccgcccgg
    28201 tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat tgcgtaaact
    28261 ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag cgcaaaccaa
    28321 gaatttgttg cgccagaccg ttacaccaga aaataccgct ctcttccgtg gtcagcacca
    28381 ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg cccagttcac
    28441 ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc ggttcccagc
    28501 taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat gaaaggcgca
    28561 ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa aaccagggca
    28621 ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc agctccagca
    28681 ccagcctttt ccacgacagc cgttccagca cgtaagatac tcccgttaag aaatcataag
    28741 ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga tatcctgtac
    28801 cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta cgcaggcgac
    28861 gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag acgtggttta
    28921 acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc agcagtttaa
    28981 attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga gatgtcgggt
    29041 cgagacttaa tccctgcatc tcaatcacct cttccaccgc cattggcgaa atacggcgca
    29101 ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa cggcttggtg atatagtcat
    29161 ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg gtcaacatca
    29221 ccactggaat atcccgggtc atcgactcgc gcttaaggtg tttgatgaac tggataccgg
    29281 agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca ttcagttgat
    29341 tcacagcaca gtcataatct tccgcttcga ccggctgaaa gccattttgt tcgagcacga
    29401 agcagaccat ttcacgaatt ggagcttcat cttctacgac cagaatacgt ctcgccatga
    29461 tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat gcgtcagatt
    29521 tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt tccgtggcgc
    29581 agaaaaaagc aaatggcaca tctgtttggg tataatcgcg cccatgcttt ttcgccaggg
    29641 aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact tctacagtaa
    29701 aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacaa cacaaaccca
    29761 tcaggtggat gcgattattg ttgccggtga tgttttcgat accggctcgc cgcccagtta
    29821 cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct gtcatctggt
    29881 ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg atatcatggc
    29941 gttcctcaat actaccgtgg tcgccagcgc cggacatgcg ccgcaaatct tgcctcgtcg
    30001 cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgtccgc gtgacattat
    30061 taccagccag gcggggctta acggtattga aaaacagcag catttactgg cagcgattac
    30121 cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc agcctctgcc
    30181 catcatcgcc acgggacatt taacgaccgt gggggccagt aaaagtgacg ctgtgcgtga
    30241 catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag ccgactacat
    30301 cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg ttcgctattg
    30361 cggctccccc attccactga gttttgatga atgcggtaag agtaaatatg tccatctggt
    30421 gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg taacgcaacc
    30481 catggcagtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac agtggcgcga
    30541 tgtatcgcag gagccaccag tctggctgga tatcgaaatc actactgatg agtatctgca
    30601 tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag tattgctggt
    30661 acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg aaaccctcag
    30721 cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagaac tggatgaatc
    30781 gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cataccctcg ccggagaaca
    30841 cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa aggcgaatgg
    30901 aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgctat taccggccca
    30961 acaggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta tcacgaaact
    31021 ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga taccgccgaa
    31081 tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt accgtgcatt ctggagccag
    31141 aatcgggcgc gtaaccaacc cgacggtaat ttgcaggtgc cacgcgtaga gctggcgcgc
    31201 tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact gacagcgacg
    31261 ttaaccgggc tggattacgg gcgcttcact cgttcgatgc tgctttcgca ggggcaattt
    31321 gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga gttaaccggc
    31381 actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc ggcccgcaca
    31441 gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc ggaacaagtg
    31501 caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt aattaccgcg
    31561 cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt gcagcaagaa
    31621 gccagccgcc gtcagcaggc cttgcaacag gcgttagccg aagaagaaaa agcgcaacct
    31681 caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca ctgggaacgc
    31741 atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga agtaaatact
    31801 cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc gaagcagtca
    31861 gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca cgaccgcttc
    31921 cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca aaccagcgat
    31981 cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa acttaatgcg
    32041 cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctagcgccct ggcgcaacat
    32101 tccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat tgttccccaa
    32161 caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga acagacgcaa
    32221 cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca gcaacttgcc
    32281 gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc tcaacgtgca
    32341 cagttacagg cgggtcaacc ttgtccactt tgtggttcca ccagccaccc ggcggtcgag
    32401 gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct ggaaaacgaa
    32461 gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc attaacgaag
    32521 cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca agcacttact
    32581 caacaatggc aagccgtcac gaccagcctc aatatcacct tgcagccaca ggacgatatt
    32641 caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact cagccaacga
    32701 catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta tcaacagcaa
    32761 attgaacaac gccagcagca gctcttaacg gcattgacgg gttatgcact gacattgcca
    32821 caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca gagctggcag
    32881 caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac gccgattctg
    32941 gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc gctggataac
    33001 tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac gttacagcaa
    33061 caggatgttc tggcggcgca aagtctgcaa aaagcccagg cgcagtttga caccgcgcta
    33121 caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga tgaacaaaca
    33181 ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca ggcgcaaacg
    33241 ctggtcacac agacagcaga aacgctggca cagcatcaac aacaccgacc tgacgggttg
    33301 gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca ccaaaagttg
    33361 cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga tgcagataac
    33421 cgtcagcaac aacaaacctt actgcagcaa attgctcaaa tgacgcagca ggttgaggac
    33481 tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg caagtttgcc
    33541 caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac ccggctgcac
    33601 gggcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt tgttgatacc
    33661 tggcaggcag atgcagtacg cgatacccgt accctttccg gcggcgaaag tttcctcgtt
    33721 agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg tattgactcg
    33781 ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac cgcccttgat
    33841 gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca cgttgaagcg
    33901 atgaaagagc gtattcctgt gcagatcaaa gtgaagaaga tcaacggcct gggctacagc
    33961 aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa tacagagggg
    34021 cgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta ttggctacgg
    34081 tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt ttatctttgg
    34141 ctctgggcac gtttggtttg gggatggccg aatttggcat tatgggcgtg ctcacggagc
    34201 tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg tattatgcac
    34261 tgggggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac tcactcaaac
    34321 atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc acgctctctt
    34381 cgtcttacct gatgcttgcc attggtcgcc tggtttccgg ctttccgcat ggcgcatttt
    34441 ttggcgtcgg cgcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc accgccgccg
    34501 tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg ctgggaacgt
    34561 atttaagtca ggaatttagc tggcgttaca cctttttatt gatcgctgtt tttaatattg
    34621 cggtgatggc atcggtctat ttttgggtgc cagatattcg cgacgaggcg aaaggaaatc
    34681 tgcgcgaaca atttcacttt ttgcgcagcc cggccccgtg gttaattttc gccgccacga
    34741 tgtttggcaa cgcaggtgtc tttgcctggt tcagctacgt aaagccatac atgatgttta
    34801 tttccgtttt ttcggaaacg gcgatgacct ttattatgat gttagttggg ctagggatgg
    34861 tgctgggaaa tatgctaagt ggcaggattt caggacgtta ttcaccactg cgcattgcag
    34921 cagtgactga ctttatcatt gtactggcac tgctgatgct ctttttctgc ggcggcatga
    34981 aaacaacgtc gcttattttt gcttttattt gttgcgcggg attatttgcc ctttcagcac
    35041 ctctgcaaat attgttactg caaaacgcca aaggcggaga gttattaggt gccgcaggtg
    35101 ggcaaatagc gtttaacctc ggtagcgccg tcggcgcata ttgcggaggt atgatgctga
    35161 cgctggggct ggcatataat tacgtggcgc tgcctgccgc cctgctttcg tttgctgcga
    35221 tgtcatcgtt gctgctgtat ggtcgctata agcgccagca agcggcggat agtccggtgc
    35281 tggcgaaacc actggggtag attactgtca cggtgcgcgt tgcttattgc cggatgcggc
    35341 gtgaacgcct tatccgccct acgcggttct ggcacatttt gtaggcctga taagacgcgg
    35401 caagcgtcgc atcaggcatg atgcgccaat tgcctacgtt ttttactctt gtggccataa
    35461 ccacgcagcg ccgcgtacgc cgctggaatc accgtgcttc gccttacgca ccggcgtttc
    35521 acattcgccg ccgaagacaa attgtttaat caactgccca accgtttgat ataaacggtc
    35581 tacattgctc atcccgcccc ccaggacaat cacatccgga tcgagaatat tcacgacatg
    35641 tgccagcgat tttgccagcc gcagctcgta gcgacgcaat gccagttccg ctaccggatc
    35701 gctttcttca accaggcgga taatttcact gcctttcagc gcatgtccgc tcaaacgacg
    35761 ataatccgtc gcgaatcccg tgcccgaaat aaaggtttca atacaacctt gtttaccgca
    35821 ataacaaggg acttcctcgc gataacgcag ctcgtcttca tccatccacg gtagcggatt
    35881 gtgtccccac tcgcctgccg tgccattgcc gccgatatgc gcccgcccat ttaatgccac
    35941 gcccgcgccg catcccgtgc cgataatcac ggcaaatacc gtctgcgctc ccgctgccgc
    36001 gccatctact gcttctgaaa ccgccagaca gttggcatca tttgccagcc gcacttcccg
    36061 ctgcaacctc gcgcttaagt ctttatcgaa tggctgaccg ttgagccagg ttgaattggc
    36121 attcttcacc acaccggtgt agggcgaaat cgaacctgga atgcccatac ctaccgttcc
    36181 gcgctgccct gtcgcctgct ccgccatatc aaccaacgtg gcgatcgttt caatagtctg
    36241 ccggtaatca tcacgcggcg tgggcagacg atggcggtac aactgctccc ctgcatcgcc
    36301 cagtgcaatc acttcagttt tggtgccgcc taaatcgata cctatacgca ctgtactctc
    36361 cttatttttt tcaatatcaa tagcgtagag acggacaacc ggattggcaa tgcaaggccg
    36421 ccgacaattc gttatcatgc ccgctaaatt taacgacaag gccgtggaaa ttatcatgct
    36481 gtggttcaaa aatttaatgg tttaccgtct tagccgcgag atttcgctgc gtgcagaaga
    36541 gatggaaaaa cagctagcct cgatggcatt taccccatgc ggcagccagg acatggcgaa
    36601 gatgggctgg gttcctccga tgggatcgca cagcgatgcg ttaacgcacg ttgccaatgg
    36661 tcaaattgtt atctgcgcgc gcaaagaaga aaaaatcctc ccgtctccgg tgattaaaca
    36721 ggcgctggaa gcgaaaatcg ccaaactgga agcggaacag gcgcgtaagc tgaagaaaac
    36781 cgaaaaagat tcgctgaaag acgaagtgct gcactctctg ctgccgcgtg ctttcagccg
    36841 ttttagccag acaatgatgt ggatcgacac ggttaacggt ttgattatgg tggactgcgc
    36901 cagtgccaaa aaagcggaag atacgctggc attactgcgt aaaagcctgg ggtcgttacc
    36961 ggttgtacca ttgagcatgg aaaacccgat tgaactgacg ctgaccgaat gggttcgctc
    37021 cggtagtgcg gcacagggct tccagctgct tgatgaggcc gagctgaaat cgttgctgga
    37081 agatggcggc gtgatccgcg cgaagaaaca agatctgacc agcgaagaga tcaccaatca
    37141 cattgaagcc ggaaaagtgg tgactaaact ggcgctcgac tggcagcagc gcattcagtt
    37201 tgtgatgtgc gacgatggtt cgctcaagcg tcttaagttc tgcgacgagc tgcgcgatca
    37261 aaacgaagat atcgaccgtg aagatttcgc cctgcgtttt gatgccgatt tcatcctgat
    37321 gactggtgaa ctggcagcgt taattcaaaa cctgattgaa ggattaggtg gcgaagcaca
    37381 acgttaattg ctgattttcc tttaatgccg gatgcgacgc ctgccgcgtc ttatccggcg
    37441 tacgaagcca caccaggcat ataattattc gctacggcga gcaataattt ttagcgcagc
    37501 aatattatgc gttttacgcc gtaactgatc taatcgcctc atccctattc gcctgtcagc
    37561 agcacgcaga atattcacta acccccattt atcgctggta aattgttttg ttaacagctc
    37621 cgcttttggg gtatcaatat attcacgcaa taaatcagaa attgaacaaa tatccgtcag
    37681 gtaaggatac cagcaagttt caccgtgaaa attacgcact ccagcgttac ctgcaataaa
    37741 gtctttcggg taatcccaga tcacgttttt atccaacgtg atccaataac gcggtaaatc
    37801 ggtacttcca ccattttggc tgcgcatgag ataacgggta cagtgaattt gcaggttgat
    37861 cgttggcgtc agtaagtcat agatctcccg ctgtaacttg ctccatggat gttgtgtcat
    37921 tgtttttcct caagccggtg acgcgcttca ctgcgaattt tccgcgttgc cccggttttc
    37981 actaaatcac gtaactgcga aagatcgggt tgttcgacaa ataagagtaa tgatagatcc
    38041 tcttttaacc acgccgtttt cacatcggca gccagctgtg gattattaat agccagcgac
    38101 cgatcctgag actcagtcaa tgtcgttaac aacgacgccg gggtatgtgc gttgcgtaat
    38161 gccgtttggc gcaaatgatc attgcgactt tgcagcaatg cacgccacca ttcatcatct
    38221 gatttatctt ttatcgtagc cagttccgca aaaaatacct ggagatcttc actgatattt
    38281 atatcaacct tgatgaatta tgaatatatc ggaaattgaa aaaatcgcag caaagga
//
LOCUS       DAEEKQ010000023        37988 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.023, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000023 DAEEKQ010000000
VERSION     DAEEKQ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 37988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37988
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.023"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(268..885)
                     /gene="tdk"
                     /locus_tag="KLK57_001137"
     CDS             complement(268..885)
                     /gene="tdk"
                     /locus_tag="KLK57_001137"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HBE0891569.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1490..1903
                     /gene="hns"
                     /locus_tag="KLK57_001138"
     CDS             1490..1903
                     /gene="hns"
                     /locus_tag="KLK57_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HBE0891570.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2047..2955)
                     /gene="galU"
                     /locus_tag="KLK57_001139"
     CDS             complement(2047..2955)
                     /gene="galU"
                     /locus_tag="KLK57_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HBE0891571.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="KLK57_001140"
     CDS             complement(3157..4170)
                     /gene="rssB"
                     /locus_tag="KLK57_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HBE0891572.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="KLK57_001141"
     CDS             complement(4262..5167)
                     /gene="rssA"
                     /locus_tag="KLK57_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HBE0891573.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5280..5738
                     /locus_tag="KLK57_001142"
     CDS             5280..5738
                     /locus_tag="KLK57_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HBE0891574.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5788..6630
                     /gene="purU"
                     /locus_tag="KLK57_001143"
     CDS             5788..6630
                     /gene="purU"
                     /locus_tag="KLK57_001143"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HBE0891575.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMAEAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6790..6874
                     /locus_tag="KLK57_001144"
     tRNA            6790..6874
                     /locus_tag="KLK57_001144"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6824..6826,aa:Tyr,seq:gta)
     gene            6922..7053
                     /locus_tag="KLK57_001145"
     ncRNA           6922..7053
                     /ncRNA_class="other"
                     /locus_tag="KLK57_001145"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            7101..7232
                     /locus_tag="KLK57_001146"
     ncRNA           7101..7232
                     /ncRNA_class="other"
                     /locus_tag="KLK57_001146"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7416..8093)
                     /gene="narI"
                     /locus_tag="KLK57_001147"
     CDS             complement(7416..8093)
                     /gene="narI"
                     /locus_tag="KLK57_001147"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBE0891576.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8093..8803)
                     /gene="narJ"
                     /locus_tag="KLK57_001148"
     CDS             complement(8093..8803)
                     /gene="narJ"
                     /locus_tag="KLK57_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBE0891577.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8800..10338)
                     /gene="narH"
                     /locus_tag="KLK57_001149"
     CDS             complement(8800..10338)
                     /gene="narH"
                     /locus_tag="KLK57_001149"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBE0891578.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10335..14078)
                     /locus_tag="KLK57_001150"
     CDS             complement(10335..14078)
                     /locus_tag="KLK57_001150"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HBE0891579.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVSTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFLDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14471..15862)
                     /gene="narK"
                     /locus_tag="KLK57_001151"
     CDS             complement(14471..15862)
                     /gene="narK"
                     /locus_tag="KLK57_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HBE0891580.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16201..17997
                     /gene="narX"
                     /locus_tag="KLK57_001152"
     CDS             16201..17997
                     /gene="narX"
                     /locus_tag="KLK57_001152"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HBE0891581.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDEQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLLDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17990..18640
                     /gene="narL"
                     /locus_tag="KLK57_001153"
     CDS             17990..18640
                     /gene="narL"
                     /locus_tag="KLK57_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HBE0891582.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18641..20035)
                     /gene="ychO"
                     /locus_tag="KLK57_001154"
     CDS             complement(18641..20035)
                     /gene="ychO"
                     /locus_tag="KLK57_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HBE0891583.1"
                     /translation="MSRFVPRIIPFYLLLLAAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVRVEQYFGERVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20220..20573
                     /gene="ychN"
                     /locus_tag="KLK57_001155"
     CDS             20220..20573
                     /gene="ychN"
                     /locus_tag="KLK57_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HBE0891584.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20617..21312)
                     /gene="chaC"
                     /locus_tag="KLK57_001156"
     CDS             complement(20617..21312)
                     /gene="chaC"
                     /locus_tag="KLK57_001156"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HBE0891585.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPNE
                     GPVWIFGYGSLMWNPALEFTELCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21470..21700)
                     /gene="chaB"
                     /locus_tag="KLK57_001157"
     CDS             complement(21470..21700)
                     /gene="chaB"
                     /locus_tag="KLK57_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HBE0891586.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21970..23070
                     /gene="chaA"
                     /locus_tag="KLK57_001158"
     CDS             21970..23070
                     /gene="chaA"
                     /locus_tag="KLK57_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HBE0891587.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23473..23580
                     /locus_tag="KLK57_001159"
     CDS             23473..23580
                     /locus_tag="KLK57_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0891588.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIITAAIVSWWRNRK"
     gene            24009..24116
                     /locus_tag="KLK57_001160"
     CDS             24009..24116
                     /locus_tag="KLK57_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0891589.1"
                     /translation="MTLAKFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            24544..24651
                     /locus_tag="KLK57_001161"
     CDS             24544..24651
                     /locus_tag="KLK57_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0891590.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
     gene            complement(24800..25654)
                     /gene="kdsA"
                     /locus_tag="KLK57_001162"
     CDS             complement(24800..25654)
                     /gene="kdsA"
                     /locus_tag="KLK57_001162"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HBE0891591.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(25690..26498)
                     /locus_tag="KLK57_001163"
                     /pseudo
     CDS             complement(25690..26498)
                     /locus_tag="KLK57_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
     gene            complement(26502..26894)
                     /locus_tag="KLK57_001164"
     CDS             complement(26502..26894)
                     /locus_tag="KLK57_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HBE0891592.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(26891..27724)
                     /gene="prmC"
                     /locus_tag="KLK57_001165"
     CDS             complement(26891..27724)
                     /gene="prmC"
                     /locus_tag="KLK57_001165"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBE0891593.1"
                     /translation="MEYQYWLREAISQLQASESPRRDAEILLEYVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(27724..28806)
                     /gene="prfA"
                     /locus_tag="KLK57_001166"
     CDS             complement(27724..28806)
                     /gene="prfA"
                     /locus_tag="KLK57_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HBE0891594.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(28848..30104)
                     /gene="hemA"
                     /locus_tag="KLK57_001167"
     CDS             complement(28848..30104)
                     /gene="hemA"
                     /locus_tag="KLK57_001167"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HBE0891595.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            30318..30941
                     /gene="lolB"
                     /locus_tag="KLK57_001168"
     CDS             30318..30941
                     /gene="lolB"
                     /locus_tag="KLK57_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HBE0891596.1"
                     /translation="MPQPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            30941..31792
                     /gene="ispE"
                     /locus_tag="KLK57_001169"
     CDS             30941..31792
                     /gene="ispE"
                     /locus_tag="KLK57_001169"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HBE0891597.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            31943..32890
                     /gene="prs"
                     /locus_tag="KLK57_001170"
     CDS             31943..32890
                     /gene="prs"
                     /locus_tag="KLK57_001170"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HBE0891598.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            33014..34693
                     /gene="dauA"
                     /locus_tag="KLK57_001171"
     CDS             33014..34693
                     /gene="dauA"
                     /locus_tag="KLK57_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HBE0891599.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(34748..35026)
                     /gene="ychH"
                     /locus_tag="KLK57_001172"
     CDS             complement(34748..35026)
                     /gene="ychH"
                     /locus_tag="KLK57_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HBE0891600.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            35303..35887
                     /gene="pth"
                     /locus_tag="KLK57_001173"
     CDS             35303..35887
                     /gene="pth"
                     /locus_tag="KLK57_001173"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HBE0891601.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            36004..37095
                     /gene="ychF"
                     /locus_tag="KLK57_001174"
     CDS             36004..37095
                     /gene="ychF"
                     /locus_tag="KLK57_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HBE0891602.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRVGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
BASE COUNT         9462 a         9842 c         9286 g         9398 t
ORIGIN      
        1 tctggggccg tttttttgtt tacccaaagc aacttttcca taaaccgaca gcattagcct
       61 tcatcatatt tgcgacgatg tataacgcct aaacacaggg atattgtact ttacaggtca
      121 caagtcaacg tcggtgctta agagccctgt gaggcgtata gcggcgttaa aaaactgccg
      181 agaagggtat atagcccgga agaagtgcgt aaaacgaact gacaggataa aagtgccctg
      241 ctcaccctgt cagtaaagaa attcttatta atcgtggcga tgcctttcct gaatagccgt
      301 taatgagccg acttgtaacg cctctttata gtgtttacgg catacagaaa cgtatcgttc
      361 attaccacca attaccacct gctcaccttc gttataaggt ctgcctgctt gatcaagacg
      421 cagcaccatg cttgctttac ggccacaaaa acagatggtt tttaattcaa ccagtttgtc
      481 ggaccatgcc agtaagtatt ggctgccaat aaataattca cctcgaaaat cggtacgtaa
      541 accataacaa agtacgggta tatcgagttg atcgacaacc tccgataatt catatacttg
      601 ttgtctggtt aaaaactggc attcatcaac cagtacgcaa tgaattgcct gctgttcatg
      661 ttccgcacga atctcatcaa ataatgatga attttggtta aataattttg caggcgatga
      721 caaacctata cgcgaactga ctttcccggc accaaagcga tcatcaattt ctgccgtata
      781 tacgacagtg cgcatgccgc gttcctggta attgtatgaa gattgcaaca atgctgtaga
      841 cttacccgca ttcattgcgg aatagtagaa atatagctgt gccatcagcc acaggccctc
      901 aatgatatga aataaagttg gctggagttt atcataattc gtcagttttt cagctcaaca
      961 gtatgcacag aagtgggtat atgcgttctc ccttacgaag ccttgcataa tccttctgag
     1021 ctatcattac aactgcctcg cttgttataa gcgggtaaat gactactggt aactattcac
     1081 aatctttaac ctgttgcgca agtaatagcc ctctgttgac ctccaggaga tagtgcaata
     1141 ctaagtccat gctcttattg cgactgttct acttttcatc attcgcttaa tagggaattc
     1201 tcgtaaacac aactaataca gaagactgaa aggtcgtcag cctacgataa tctccccata
     1261 aaatgtgaca tgaatcagga agttttaacc tcacgtgctg cgaaatcatc ggtgtaaata
     1321 gggctatatg ccgcgtcttt tctggctaat tttatgaaaa gatatttatt ggcggcacaa
     1381 aataaagaac aattttgaat tccttacatt cctggctatt gcacaactga atttaaggct
     1441 ctattattac ctcaacaaac caccccaata taagtttgag attactacaa tgagcgaagc
     1501 acttaaaatt ctgaacaaca tccgtactct tcgtgcgcag gcaagagaat gtacacttga
     1561 aacgctggaa gaaatgctgg aaaaattaga agttgttgtt aacgaacgtc gcgaagaaga
     1621 aagcgcggct gctgctgaag ttgaagagcg cactcgtaag ctgcagcaat atcgcgaaat
     1681 gctgatcgct gacggtattg acccgaacga actgctgaat agccttgctg ccgttaaatc
     1741 tggcaccaaa gctaaacgtg ctcagcgtcc ggcaaaatat agctacgttg acgaaaacgg
     1801 cgaaactaaa acctggactg gccaaggccg tactccagct gtaatcaaaa aagcaatgga
     1861 tgagcaaggt aaatccctcg acgatttcct gatcaagcaa taatcttttg tagattgcac
     1921 ttgcttaaaa tcccgccagc ggcgggattt tttattgtcc ggtttaagac aatttaataa
     1981 gaataagcta taaaaaaacg gcgtcgattg ctcaacgccg tttcgtggat aacaccgata
     2041 cggatgttac ttcttaatgc ccatctcttc ttcaagccag gctttaaatt ccgtgccaag
     2101 ggtgttatga cgaataccgt attcaacgaa ggcctgcatg taacctaatt tattaccgca
     2161 gtcatggctc ttccctttca tatgataggc ttccaccgtt tctttttcga tcagcatatc
     2221 aattgcgtcg gtgagctgaa tttcatcacc agctcccgga ggggtttttg ccagcaacgg
     2281 ccaaatatcc gcgctaagta cgtaacgacc cacaatagcg agattagacg gcgcaacatc
     2341 cgctttcggt ttttctacca caccaaccat cggtacgctt tcacccggcg ctaattcaac
     2401 gcctttgcaa tccacaacgc catatgcggt cacatcagca accggttcaa ccatgatctg
     2461 gctatgaccc gtttcatcaa agcggcggat catctctgcc aggttatcct gtgacaaatc
     2521 ggattcatat tcatccagaa taacatcagg caaaataaca gctaccggtt catcacccac
     2581 taccgggtga gcacacaata ccgcgtgtcc caggcctttc gccagaccct gacgaacttg
     2641 cataatagtc acgtgcggtg gacaaataga ctgcacttca tcaagcagtt gacgttttac
     2701 acgtttttcc agcattgctt ccagttcaaa actggtatca aagtggtttt caatagagtt
     2761 tttagatgag tgtgtaacca gcacaatttc agtaatgcca gccgcaatac attcattcac
     2821 gacgtattga attaatggct tatcgacaag tggcagcatc tctttcggga tggctttcgt
     2881 cgccggcaac atcctggttc ctaatcccgc aacggggata acggcttttt tgactttcgt
     2941 attaatggca gccatttaaa ttctcctgga ctgttcgtgt tttgaacgtg ttcatatttc
     3001 tgtatcgcat cccagtatat cagcaccccg aaggggctaa gttctgaaaa ggaggcgagt
     3061 tagcataatt aagacaatta cttatacgaa tggcgacgat agtaccacca gccgacatta
     3121 gcaggtaatg caaatttagc ccgcgttatc gtttgctcat tctgcagaca acatcaagcg
     3181 cagtcgacca ccggttcccc atatttggca ttgccaggca tcgcatcgct ggctcaattg
     3241 attcaaataa gcgttaccta aagtgcctaa cggaacacca ttactgattt gcacctggtg
     3301 ttcgccggta tttaacgtcg cattcagacc cgcagaaacc agaatgagat ttttcagttc
     3361 gcgatgataa tagccaacta atagcggaaa ctgccccggc agattggcct gacggagtaa
     3421 atggtttacc tgcttcaata acgcgcccaa ctctggcaac cgttgatttt ggtgtgcaag
     3481 ctgttcctgt aataatccgt taaacaatgc gcgtaataac aaggcagcaa gtacgccatt
     3541 atgtccagct cgggtgacat caaggcaata aaatgccaga tcgttttccg aaagtgcggc
     3601 aatatcaagc accaggccgg gtttgtccgc ggcaaccaat tgacgataat taacccggca
     3661 atgggaaatc acctgctgaa ccggcggttg tagttcctgt aataatttcg ccgctgcggc
     3721 agggttatca accattgcat cccagtcgcg aaaaagcctt tcctcttcct caacgcgcga
     3781 attaaacatg ctgggataga gacaggcaaa aaccatctcg cgcaagcgat tcagatcttt
     3841 aactggtttc agcaaaacat cttcaacgcc cagacgtaac gctttggcaa tatctgccat
     3901 attttcagtg gcagatatca ccagaactgg ggtctggtcg cctctgttac gtatatgctc
     3961 cagcagttta agcccgttca ttcgtggcat cgcgatatca catatcatca ggtctggagt
     4021 gaaacctccc agcaactcaa gggcatccac cccatcagcc gccagtaccg ttgtcgctcc
     4081 caatgaggaa aaccatgaat ccagaagcga gcgaaatacc tgctcatctt caacaatgag
     4141 aatctgtttt ccgaccaatg gctgcgtcat gttctctccc ctgactggct ttactcaata
     4201 gtggcatgct attgccacct gcgcctgtca gaattaactt aagtgtagat aaaaattctg
     4261 gtcaaatgtt ggtgcgtacc aacggcaaaa gttcgtccat tttcctttcc actgccagct
     4321 gtccggccgc aatggcagcg tgcgcgcgat ggaaatcaag cgtagatatt tgcgggcaaa
     4381 caggttgaat cagaatatcg ggcggatcac ctgccatgcg gttcctttta aggcggttct
     4441 ccaacacctg gatagacgtg gtcataatct ctgttgccgt tggcgctgtc acagcccgac
     4501 gtgtcgttat gctgcctaac ctttctttca gacgcgcatg ccacggtaga gaatcaccat
     4561 tctcgctatt ttcttcactg acattaaagg agagcagatc ttgttgcatc aaatgagcat
     4621 cgtgctgcag gtcgaccgct atcacaatat cagcccccaa tgcacgcgta agagaaatag
     4681 gaattgggtt aactactgcc ccatcaacca gccagtagcc gttatgtgca acgggtgcca
     4741 tgagtcctgg aatactgcat gatgcgcgaa tagcaagatg gagatcgcct tcagtaaacc
     4801 ataattcacg ccccgtactt aaattggtgg caacagccgc aaagcgacgg gaacaatttt
     4861 cgatctctgt ttccggcatt atttcgcgat attgattgaa gacacgctcg ccgcgcagta
     4921 acccaccgcg ctgccaggag aggtccatca ggcgtaaaac gtcccaataa ctgaaagagg
     4981 ttacccaatc ttccagcgca gataatcgat cgcatgcata ggccgcgccc accagcgaac
     5041 caattgaaca tcctgcaacg atatcaattt caatacccac tttttttagc gcattaataa
     5101 cgccaatatg cgaccaacct ctcgccgcgc cagatcccag cgccagccct atttttatct
     5161 ttctcattat tcccgcaaga ttcccctgga atgcaatcgt agccacatcg ctactgctcg
     5221 gttaacatag tgctactcag gcggttaata cgccgttatt gtttccaggg agattatttg
     5281 tgtctcagct ttgtccctgt ggtagtgctg tcgagtatag cctatgttgc cacccttatg
     5341 tgtctggtga aaaggttgca cctgatccag aacatctcat gcgttcgcgt tactgcgctt
     5401 ttgtgatgca agacgcagat tatttaataa agacctggca tccctcttgt ggagcagcgg
     5461 cgttacgtgc cgaattgatg gccggatttg cgcatactga atggttggga ttgaccgtat
     5521 ttgaacattg ttggcaggac gccgacaata tcggatttgt cagttttgtt gctcgattta
     5581 ccgaaggtgg caaaacaggt gcaataattg aacgttcgcg atttttgaaa gaaaacggtc
     5641 aatggtacta tattgacggt acacgtccgc agtttggtcg caacgatccc tgcccttgtg
     5701 gttcaggtaa aaaatttaaa aagtgctgcg gccaataatg gttgacggta cggtttagca
     5761 aacactctca acaaggtttt tccagcaatg cattcactcc aacgtaaagt tctgcgtact
     5821 atttgtccgg accaaaaagg tctgatcgca cgtattacca atatttgcta caagcacgag
     5881 ttaaatatcg tacagaacaa tgaatttgtt gatcaccgta ccgggcgctt ttttatgcgc
     5941 acggaactgg aagggatttt taatgattcc accctgctgg cggatctcga tagcgcattg
     6001 ccagaaggct ccgtgcgtga gctgaatcct gccggtcgtc gccggatagt gattctggtc
     6061 actaaagaag cgcattgcct tggcgatttg ttgatgaaag ccaattacgg cggcctggat
     6121 gtcgaaatcg cggcagttat tggtaaccac gatactttac gttctctggt tgagcgtttt
     6181 gatattccgt ttgagctggt aagccatgaa gggttaagcc gcaacgagca cgatcaaaag
     6241 atggcggaag ccattgatgc ttatcaacct gactatgtgg tgctggcgaa gtatatgcgg
     6301 gtattaacgc cggaatttgt ggcacgcttc ccgaataaaa tcatcaatat tcaccattca
     6361 ttcctgcctg cgtttatcgg cgcacgtcct tatcaccagg cctatgaacg tggtgtgaag
     6421 attattggcg caaccgctca ctatgtgaat gacaatctgg acgaaggccc aatcatcatg
     6481 caggacgtta ttcatgtcga tcatacctac acagctgaag atatgatgcg cgcaggtcgt
     6541 gacgtcgaga aaaacgtctt aagtcgtgcg ctatacaaag tactggcaca gcgcgtcttt
     6601 gtttacggta atcggacgat tattctttaa tcgccagcaa aaataactgg ttacctttaa
     6661 tccgttacgg atgaaaatta cgcaaccagt tcatttttct caacgtaaca ctttacagcg
     6721 gcgcgtcatt tgatatgatg cgccccgctt cccgataagg gagcaggcca gtaaaaagca
     6781 ttaccccgtg gtggggttcc cgagcggcca aagggagcag actgtaaatc tgccgtcatc
     6841 gacttcgaag gttcgaatcc ttcccccacc accatcactt ttcaaaagtc cgtaaactct
     6901 caaacgaatc cgcaattaaa tattctgccc aagcggggaa ggatgagaag cttcgaccaa
     6961 ggttcgactt gagcgccagc gagagagcgt tgccgcaggc aacgacccga agggcgaagc
     7021 gcgcagcgct gagtaatcct tcccccacca ccatcacttt tcaaaagtcc gtaaactctc
     7081 aaacgaatcc gcaattaaat attctgccca agcggggaag gatgagaagc ttcgaccaag
     7141 gttcgacttg agcgccagcg agagagcgtt gccgcaggca acgacccgaa gggcgaagcg
     7201 cgcagcgctg agtaatcctt cccccaccac catcgcttcc ttaaataatc aaaacctgtt
     7261 actccgagct aattttgccc gatggcacca cgtttaccgg gcctacaata tcctcccctg
     7321 taggtcggat aagcgaagcg catccgacaa ctcaccccgc accgtgccca taaaaaaacc
     7381 ccgccgaagc agggtctatg tgaactaaaa ttcgcttagt gacgagcgcg caccagctgg
     7441 tacttacgtg tcagatactc caccggtacg ctccagatgt gtaccagacg cgagaacggg
     7501 aacagcagga ataacgtcat ccccagcacc aggtgcagac ggaagataaa cgccacacca
     7561 tcgaggtgtt gagaagcgcc accgtggaag gtcaccaccg actgcgccca gccaaccagt
     7621 ttcatcatct cgctaccgtc catatgctga gcggagaacg gaatggtcaa caggcccagc
     7681 gcgcactgga taacgagcag cgacaggatc aggatatccg ctccggtagt ggttgcacgc
     7741 acgcgtgggc tgaacagacg acgcttcagc aacagcacgc cgccaatcag acacagcacg
     7801 ccgctggcac caccagcaaa catcgccatt ttctgtttca cttcaatcgg cagccaggct
     7861 tcatacatcc agtgcggcgt cagcataccg aagaagtgac caacaaaaat ccccagaata
     7921 ccgatatgga acaggttcga cgccaggttc atccctttgc gatccagcat ctggctggac
     7981 gccgcgcgcc aggtgtactg cccgtagtca taacgcagcc agctaccaat caggaagacc
     8041 gccccggcta tgtacgggta gatatcaaag aagaacatat tcaggaattg cattagtgct
     8101 gtcctccggt ggtgatattc agatattgcg gcgcgacggc accggcaaag cgacgctgat
     8161 gagcagtgat tgctgaatca ccgcagcctt tgtcagcaaa gaatttaacc tgctcttctt
     8221 cccagacagc atccagcgcc tgcggcgtat catcgcgcgc ttcgtcggca attttttccg
     8281 ccactttgtc gctgtcgata gcggtattgg ccaatttcag cagcagatca aacatcacgg
     8341 cataacggct ttcacgctgt tgcagacgcg cgctcagcaa tgccagaatc ggcgcgatat
     8401 ctttcaaacc ttccacggct tcgctttgcg gcagctgcga caggtactcc agatacagcg
     8461 gcagatggtc cggtaattcg cggctgttta actgcaagcc gtgctgctcg tactgcgcca
     8521 gcaggtccac catcgcctgg ccgcggtcac gggattcacc gtgaacatgt tcaaatagca
     8581 acagtgacgt ggcgcgacca cggtcgaaca gttcgctgta ctgggcctgg gcatcgagcg
     8641 gatccatcgc cgttaaatcg cgcaggaaaa tgcccagcgc atgggcatct tctttcgaca
     8701 gatttttcga cgcagcaatc gcctcaaaca tctcttgttg atgctgccat aaggcagcat
     8761 ccggatattc aaggagacgc gatacaatca cgagttcgat catggatgcg gctccgtttt
     8821 gctggtcaca tcgatggcat cgatacgacg gctgttgaac agattgaatt tggtatctga
     8881 accgtggcag ccatcaccaa aggtaaagcc gcagccattt ttctccggga aggcttcccg
     8941 tgccagttca cgatgactac tcggcaccac aaagcgatct tcgtagttag caatcgccag
     9001 ataacggtac atctcctgtg cctgggcttc ggtcagaccg acctcttcca gcgcacgggt
     9061 atcaacttta ccgtcaacgg tttcagcacg tttgtaatga cgcatcgcca gcatacgttt
     9121 cagtgcgcgc agtaccggtt tggtatcacc ggcggtcagc agattcgcca gatactgtac
     9181 cggaatacgc aagctttcga cgtctggcag aatgccgttg ctacccagct cacccgcgtc
     9241 tgctgcagac tgaatcggag acagaggcgg cacgtaccag accatcggca gtgtgcgata
     9301 ttctggatgc agcggcagcg ccagtttcca ttccattgcc attttataaa ccggcgactg
     9361 ctgtgcggct tcaataacgc tcagcggaat accgtcttta atcgcctgct cgatgacttt
     9421 cggatcgttc ggatcgagga acacctccag ctgacgctgg taaagatctt tctcgttctc
     9481 ggtgctggct gcacgttcaa tcgcgtcggc atcgtacagc agcacgccaa gataacggat
     9541 acgaccgaca caggtttctg agcacacggt cggctgaccc gcttcaatac gcggatagca
     9601 gaagatgcac ttctcagact taccgctctt ccagttgaag tagatttttt tgtacgggca
     9661 tccggtgatg cacatacgcc agccacggca tttatcctgg tcgatcagga cgatgccatc
     9721 ttcttcacgc ttgtaaatcg caccgctcgg gcaggtcgcc acacatgccg ggttcaggca
     9781 gtgttcgcac aggcgcggca aatacatcat aaaggtgttt tcgaactggc tatacatcgc
     9841 cttctggatg ttgtcgaagt tcttgtcttt cgccagtttg tcaaactcac cacccaggtc
     9901 atcttcccag ttcggccctt tttcgatttt cgccatccgt tccccggtaa tcagcgaacg
     9961 cggacgggca atcggctgcg atttgctgcc ttccggcgcg gtatgcaggt tctgatagtc
    10021 aaaatcgaac ggctcgtaat aatcgtcgat ccccggcaga tgcgggttag cgaagatttt
    10081 acccagcaac atggcacggt tacccatgcg cggctgcagt ttgccgttga ttttacggat
    10141 ccagccgcct ttgtattttt cctggttttc ccagtcagtc gggaagccct gacccggctt
    10201 ggtttccacg ttgttgaacc acgcgtattc cacgccttca cggctggtcc agacgttttt
    10261 acaggtaact gaacaggtgt ggcagccgat gcacttatcg agattcagca ccatgccgac
    10321 ttgtgaacga attttcattt tacgctctcc tgtacctggt cattgccttc gccatctaac
    10381 cagtcaatgt tcttcatctt acgcactaca acaaactcat cgcggttaga acctacggtg
    10441 ccgtagtagt taaagccgta tgccagatgg gcatagccgc cgatcatatg cgtcggtttc
    10501 ggcgtgatac gggtgaccga gttatggata ccaccacgct gttgggtaat ttccgaacca
    10561 ggcaggttaa cgatacgttc ctgcgcgtgg tacatcatgg tcatccctgc cggaacacgc
    10621 tggctgacaa ccgcacgggc agtcagagca ccgttgctgt tgaatacttc aatccagtcg
    10681 ttatcggcga tacccagatc tttggcatcg gcttcactca accagaccac cggaccaccg
    10741 cgacctaaag tcagcatcag caggttgtcg ctgtaggtgg agtggatacc ccacttctgg
    10801 tgcggcgtca ggaagttgag cgctttttcc gggttgccgt tggatttctg gcctatcact
    10861 tctttcaccg aacgggtgtc gatcggcgga cgataaacca gcaggctttc accgaaatca
    10921 cgcatccact ggtgatcctg atacagttgc tgacgaccag agagcgtacg ccatgggatc
    10981 agctcgtgaa cgttggtgta accggcgttg taagaaacgt gttcatcttc cagaccagac
    11041 caggtcgggc tggagataat tttgcgcggc tgtgcctgaa tatcgaggaa gcggatcttc
    11101 tcgtcttctt tattcagcgc cagatgcgta tggtcacgac cggtaaattc gctcagggca
    11161 gcccaggctt tcacggctac ctgaccgttg gtttccggtg ccagtgtcag gatcatctct
    11221 gccgcatcaa ttgcggtgtt cagcatcggc tggcctttcg ccggaccttc cgctttggtg
    11281 tagttgagct tacgcagcag atccatctcg ctctgggtgt tccaggcaat ccctttaccg
    11341 ccattaccga ttttctccat caacgggcca atagaggtaa agcgttcgta agtcgccgga
    11401 taatcgcgct ctacgaccat aatgtgtggc gcggttttac ctgggatcag gtcgcactcg
    11461 ccttttttcc agtctttcac atccagcggc tgcgccagtt cagcggcaga gtcatgctgg
    11521 ataggcagcg tgacgatgtc ggtttcttta cccagatggc cgacgcacac ttcggagaat
    11581 ttcttcgcga tggctttgta gatttcccag tcgcttttcg cttcccaggc cggatcgacc
    11641 gccgcagaca gcgggtgaat aaacggatgc atatccgaag tattcatgtc gtctttttcg
    11701 taccaagtcg ccgtcggcaa aatgatgtcg gaatagagac aggtgctcga cagacggaag
    11761 tccagcgtaa ccaccagatc cagcttgcct tccagaccat tgtcctgcca gtccacttct
    11821 tccggcttca cgccgccctg ttgccccaga tctttaccct ggataccgtg ctccgtcccc
    11881 agcaggtact tgagcataaa ctcatgacct ttaccggaag aaccgagcag gttagaacgc
    11941 cagatgaaca ggttgcgcgg gtggttttta ccgttttctg gttgttctgc cgcaaaacgg
    12001 atggaaccct ctttcaggga tttcaccgta tagtccaccg gattcatccc ggctttttcc
    12061 gcttcgccag cgatagtcag cgggttagtg cctaactgcg gtgcagacgg cagccagccc
    12121 atgcgttccg cacggacgtt aaagtcgatc aagtgtccgg tatagcggga tttgtccgcc
    12181 atcggtgaca gcaactcttc cgccgtgacg gtttcataac gccactggct ggagtggtta
    12241 tagaaataag aagtgctgtt catgtgacgc gccggacgct gccagtcaag ggcaaacgcc
    12301 agcggctgcc agccggtttg cggacgcagt ttttcctgac ctacatagtg cgcccagccg
    12361 cccccgctct gaccgacaca gccgcagaaa atcagcatgt tgatcagacc acgatagttc
    12421 atatcgaggt gataccagtg gttcagcccc gcaccgacga taatcatcga acgaccgtgc
    12481 gttttatcag cgttatcggc aaattcacgg gcgatgcgaa taatctggct gcgagaaacg
    12541 ccggtaatct gctcggccca ggccggggta taagctttca catcgtcata gctggttgca
    12601 cagttaacgt cgttcaggcc acgttccaga ccgtagtttg ccagcgtcag atcataaacg
    12661 gtgctcacca gggcggtgct gccatcagcc agttgcaggc gtttcaccgg cagtttgtgc
    12721 agcagcacgt tttccagttc cactttgttg aagtgttccg tgccgtcgcc accaaagtac
    12781 gggaagccta cctcagcgat ctcatcctgg ctacccagca ggctcagttg cagctcggtt
    12841 tcttcgccag ttttgccgtc gcgctgctca agattccatt tgcccttctc gccccagcgg
    12901 aagccaatag aaccgttcgg cgcaaccatt tcgccattgg tattaaaggc gacagttttc
    12961 cattccggat tgttttcctg gcccagcgca tcaaccagat cagcagcgcg cagcatacga
    13021 cctgcagcgt agtaaccgtc gcgttcttcc agcatcacca gcatcggcat gtcggtgtag
    13081 cgacgcacat agtcggtgaa atactggctt gggttgtcga ggtggaattc acgcagcatt
    13141 acgtggccca tcgccagcgc cattgccgca tcggtgccct gtttcggtgc cagccacaga
    13201 tcgcacagtt tggcgatttc agcgtagtct ggtgtgacgg caacagtttt ggtgcctttg
    13261 taacgcactt cagtaaagaa gtgagcatcc ggggtacgcg tctgcggcac gtttgaaccc
    13321 caggcgatga tgtagctgga gttataccag tcagcagatt ccggtacgtc agtttgctcg
    13381 ccccaggttt gcggagacgc aggaggcagg tcgcagtacc agtcgtagaa gcttaagcaa
    13441 gtaccgccaa tcagcgagag atagcgtgca cccgatgcgt aagaaaccat cgacattgcc
    13501 ggaattggcg agaaaccagc aacacggtcc gggccgtagt ttttgatggt gtaaacgtta
    13561 gatgcggcga tcagttcgtt cacctcctgc caggaagaac gaacaaatcc accgcgtcca
    13621 cgcgcctgct taaagctttt cgctttatcg gcgtcttcaa tgatagaagc ccatgcctca
    13681 accggatcgc tatgcagcgc cttcgcttca cgccacattt tcatcaggcg tttgcgcatc
    13741 atcgggtatt tcaggcggtt ggcactgtaa agataccagg agtagctggc accgcgcggg
    13801 cagccgcgag gttcatggtt tggcagatcc ggacgggtac gcggatagtc agtctgctgg
    13861 gtttcccagg tgaccagacc gtttttgacg tagattttcc agctgcagga gccggtgcag
    13921 tttaccccgt gggtagagcg gacgattttg tcatgctgcc aacgctggcg atatccatcc
    13981 tcccagtcac ggttggtatt gagaagctgg ccatgcccat cggcaaaggt ttcacccttc
    14041 tgtttgaagt agcgaaaccg gtccaggaat ttactcatcg gttttctcct gtgggagcct
    14101 gtcggccctc tgataaatcg acattgctaa tgtttaaggg caaggtaacg ctctgaaaca
    14161 gcgtgggaat tgataacgat caagagtgat ggggatgaaa aataaagtaa attcctaaga
    14221 taccccttta ggataattaa cagccaccac ataacagact gtaaattaag acgttttata
    14281 cctgtagtta tttaaactat taagattttt atgcagatgg gtattaagga gtattcccca
    14341 tgggtaacat attaatggct cttaatgaaa tccggcgaaa taagccatat aatatgagta
    14401 tgttgtaact aaatgacgca taaaaaaagc gcgacatcat gccgcgcaaa ggataatcaa
    14461 atattaagat ttatttttta gaatgccgac catataccgc ccaggtaatc actacgcagg
    14521 cgatatagaa aatcaaaaat accttcattg cgccgactgg cgaacccgtt aatgccagcg
    14581 agctaccaaa cgctttcggg ataaagaagc caccaatagc gccaatcgca gagatgaaac
    14641 ccagcgccgc tgccgtgtcg gttgccgctt cacgcatcgc acgttcgtca gaaccccctt
    14701 ctgctttcac gcgatccatt gtcagtttac ggaagatcac tgaaatcatc tggaaagtgg
    14761 aaccactacc cagcccagct gtcaggaaca gcgccaggaa gaccgcgaag aacgccatga
    14821 agcttccgcc ctgcccgtca gtcggtaagg tcaggaacag caggccgctg aaaatcgcca
    14881 tcagaataaa gttcaccagc gtgacacgag ttccgcccag acggtcagat aatgcaccac
    14941 ctgcagaacg cgccagcgca ccaataaacg gcccgaagaa agcgtattgc agaatctgaa
    15001 catccgggaa ctgcgttttt gacagcatcg caaagcccgc ggagaagccg ataaaggagc
    15061 cgaaggttgc cagatacagc aggctcataa tccacagatg accccgtttg agtaccggca
    15121 actgctcctt gatggaggct ttcgaggtag caagatcgtt catgccaaac cacgccgcaa
    15181 tggtgaagat ggcaaggaac ggcacccata tccaggacgc attcgccaga tacagctcag
    15241 tcccatccgg ctgtttgacg ccctggctac caaatactgc gaaaatcgac agtgatacca
    15301 ccagcggagc aaccaactgc atgacgctga cgcccatgtt tcccagacca ccattcagac
    15361 ccagcgcgcc accctgcttc tgtttcggaa agaagaagct gatgtttgcc atactggatg
    15421 cgaagttcgc gccagcaaag ccgcatagca gagagatgat gatgaagacg ctataaggcg
    15481 tggaggtatc ctgcacggca aaacccagcc agacgcaagg aataatcaga ataccggtgc
    15541 tgaacgccgt ccagcgacga ccaccgaaga taggaaccat aaaggagtat ggaacacgta
    15601 ataacgcgcc agaaaccgaa ggcagcgcag tcaacataaa tagctgatcg gtcgtaaaat
    15661 taaaaccgac tttcggtaag ttcaccgcaa cagcgctgaa caacatccat acgcaaaacg
    15721 ccagcagcag acagggaacg gaaatccaca ggttacggct ggcaatacgt tgaccgcgtt
    15781 gttgccagaa cgcaggatct tccggtcgcc aatctgtaat gacagctcca gtagcccttt
    15841 cgggggcgga tgagtgactc atagacacct ctgatactcg tttcgatgtc tgccacctta
    15901 gtgtctgtag ctaaaggcaa tttgatgtaa atcaaacgat aagataactt tatctatcat
    15961 tgatatttat cattacccat agtgagtaca gtgatttcat aaaaattatg agatttttca
    16021 cggtgctgta aaatccctac ccttaccgat gtaaagcgac taaccacacg gcaaataagg
    16081 agtaactctt tccgggtatg ggtatacttc agccaatagc cgagaatact gccattccag
    16141 aatgtatcgt cacattcatt aaggttattg ctcatttaaa gcctgaagga agaggtttgc
    16201 atgcttaaac gttgtctctc tccgctcacc ctggttaatc aggttgcgct tattgtgttg
    16261 ctttctactg ctattggact ggcagggatg gcggtttctg gctggctggt gcaaggcgtt
    16321 cagggcagcg cccatgcgat caacaaagcg ggatcgctgc gcatgcaaag ttatcgtctg
    16381 ttggcggcag tgccattaag tgagaaagac aagcccttaa ttaaagagat ggaacaaacg
    16441 gcatttagcg ccgagttaac tcgagcagca gaacgagacg aacaactggc gcaattacag
    16501 ggtttacaag attactggcg taatgaactg atccctgcac tgatgcgtgc acaaaaccgc
    16561 gaaacggtgt cagcggatgt cagccagttt gttgccgggc ttgatcagct ggtatctggt
    16621 tttgaccgca ccacggaaat gcgcattgag acagtggtac tggtccatcg ggtaatggcg
    16681 gtatttatgg cacttttact ggtgttcact attatctggt tgcgggcgcg actgctacaa
    16741 ccgtggcggc aactgctggc aatggcgagt gccgtcagtc atcgcgattt tacccaacgc
    16801 gcgaacatca gcgggcgcaa cgaaatggcg atgcttggaa ctgcattgaa caatatgtct
    16861 gcagaactgg ccgaaagtta tgccgtactt gagcagcggg ttcaggagaa aaccgccggg
    16921 ctggagcata aaaatcagat cctctctttt ttatggcagg ctaaccgccg tttgcattcc
    16981 cgcgccccgc tgtgtgaacg cctgtcacct gtactcaacg gcttacagaa tttaaccctg
    17041 ctacgtgata tcgaattgcg ggtgtatgac actgatgatg aagagaatca tcaggagttt
    17101 acctgccagc cagatatgac ttgtgatgat aaaggctgcc agctctgccc gcgcggcgta
    17161 ttacccgttg gtgatcgcgg cacgaccctg aagtggcggc tggctgactc tcatacgcag
    17221 tacggtattt tgctggcgac cctgccgcag gggcgtcatc ttagccatga tcaacaacaa
    17281 ctgctggata ccctggttga acaactcacc gccacgctgg cgctggatcg ccatcaggaa
    17341 cgtcagcaac agttgatcgt gatggaagag cgtgccacca ttgcgcgcga actgcatgat
    17401 tctattgccc aatctctctc ttgcatgaag atgcaggtga gttgtttaca gatgcagggc
    17461 gatgcgctgc cagaaagcag ccgcgaactg ttaagtcaga tccgtaacga actgaatgca
    17521 tcctgggcgc agttgcgtga attgctcacc acattccgct tgcagctcac cgagcctgga
    17581 ttacgtccgg cgctggaggc gagttgcgaa gagtacagcg ccaaatttgg cttcccggtg
    17641 aagctggatt atcaattgcc gcctcgcctg gtgccttcgc atcaggcaat ccacttgttg
    17701 caaattgccc gtgaggcatt aagtaacgcc ctcaaacatt cgcaagcgag tgaagtcgtg
    17761 gtgacggtgg cgcaaaacga taatcaggtc aaactgaccg tccaggataa cggctgcggc
    17821 gtgcctgaaa atgccatccg cagcaatcac tacggcatga taataatgcg cgaccgtgcg
    17881 caaagtttac gaggcgattg ccgcgtccgc cgtcgtgaat caggtggcac cgaagtggtg
    17941 gtcaccttta ttcccgaaaa aactttcaca gacgtccaag gagataccca tgagtaatca
    18001 ggaaccggct actatcctgc tgattgacga tcacccgatg ctgcgaactg gcgtaaaaca
    18061 gcttatcagt atggcaccag atatcaccgt ggttggcgaa gcgagtaatg gcgaacaggg
    18121 tattgaactg gcggaatctc ttgatcccga tctgatcctg ttagatctca atatgcccgg
    18181 catgaacggt ctggaaacgc tggataaact gcgcgaaaag tccctctcag ggcgcattgt
    18241 ggtattcagc gtctctaacc atgaagaaga tgtggtcacc gcactgaaac gcggcgcgga
    18301 tggttatctg ttaaaagata tggaaccgga agatctgctg aaagcattgc atcaggcagc
    18361 tgctggcgaa atggtattaa gcgaagcatt aacgcctgtt ctggccgcca gcttgcgcgc
    18421 taaccgtgcc actactgagc gcgatgttaa ccagttaacc ccacgcgagc gcgatattct
    18481 caagctgatt gcccagggtt tgccgaacaa gatgattgcc cgccgcctgg atatcaccga
    18541 aagcacagta aaagtgcacg tcaagcacat gctgaagaaa atgaagctca agtctcgcgt
    18601 ggaagcagcg gtatgggtgc atcaggagcg cattttctga ttacggttcc cagcgcagtt
    18661 cgtcgtttga caatgcatcg aacggttcga caagcgttag cgtgatctca ttggaggaga
    18721 cacgctgccc ctggttatct tccaccacca ccgacaatcg ccagtgattg ctcgcccctt
    18781 ccccgttctg ccagtcaggc atgatcagcg tccagccctc cgcgctgttg gcttgtgcgc
    18841 ccggcgtcaa acttaatatc tgcgtatcgc cctgccaaat cagttgccga ataccgtaac
    18901 gactgcggat ttgtaatttc agcggcactg tttcgccagg ttttagatcc cacggcggtg
    18961 tcgccagaaa caccgttaac gtttttcgct gtcggtactc aagagtcggt agattatttc
    19021 gctgcggatt atcatagcga ctaccacgta acgactgact ttcggcaacc tcgcccgcag
    19081 aaagttgttt tttgagcggc acaccaaagc ggtaattaag attcagtccg aggttatttt
    19141 gattttcgcc actttcaccc tgtttatgct gggccgtcac agtgactaat ggcacagggg
    19201 tgtaatttaa tcccagactc aacgcgacgg gattgtgata acccgtacca gagttaaaca
    19261 aatcaacacg ctcaccaaaa tactgttcta ccctgacact ggtattgagg tgttgataga
    19321 acggcatgcg catccgggct gtcaggtcat acccgcgcgc catccgttgt tcctgcgtgg
    19381 ctgtctgttc atgccatgca gcaaacggct gataaaagtt tgccgatagt cgcaaatatt
    19441 cgccccacgc ttcggcacca aagcccgctc gctgaagatt ttcgtccagc aagttgtcat
    19501 aaaaagtgtt ataacccacc agccagttgc cgcgcgccca gcgttgccca acgcccacat
    19561 tgctcaccaa cccatcatcc tgctgagtaa gaccaagctg gctccaggtg agataacgat
    19621 cattatcttg taacggcaca aaccagcttc cacgactgcc ggtgaaatgt ccttcgttat
    19681 ccactttgac gtcaacactg gcatttcccc acggtgatag ccaggactct acgtgctgat
    19741 taacctgttg actaagcgcg tcgcggactt ttcccaatgc gaaagctttt gcctgctcgc
    19801 cagtatccag cccgttatca ttcatactgg tttcgccgaa atctttcaca atttcagcaa
    19861 agtgtttttc cccatcatgg ttttcgggag ccatgcctaa atccggcaga ccatcattgt
    19921 tattatcaaa gggatttgcc gctttttgct cgaaggtaga ttgtgcgtta gctgtaccgc
    19981 ctgccgccag caagagtaaa taaaacggga taatgcgagg aacgaaacgg ctcaaaacgt
    20041 cggtaacttc cggtaataat gaaaaaccat agccttaaag aataacgatt actggcaact
    20101 tttgtcgtat tttctcgcgc tttcaggaaa atccttaaaa aggtgaccga ttggtcagac
    20161 gctgcagaag cagtaagata gaagatgagt gtctaattcc ctgaatacag gagtaaataa
    20221 tgcaaaaaat cgtgatcgtt gccaatggcg caccttacgg gagcgaatcc ttgtttaaca
    20281 gcttgcggct ggccattgcg ttacgagagc aggagagcaa tctggatctg cgtctgttcc
    20341 tgatgtctga tgcggtcaca gccgggttgc gcgggcaaaa accaggggaa ggctacaaca
    20401 ttcagcaaat gctggagatc cttaccgctc agaatgtacc ggtgaaattg tgcaaaacct
    20461 gtaccgacgg gcgcgggatt agcacacttc ctcttattga tggggtggaa atcggtactc
    20521 ttgtggaact ggcgcaatgg acgctgtcag ccgataaagt gctcacattt taatgtttga
    20581 gggggcgtct gacgccccac tatgagggaa gtttactcag gcgaatcccg gccgtaacac
    20641 accatccgga taattctccg ccagcagttt ttttaccgat accagcaaat cattcagccc
    20701 atcgtcctgc attcccagtt tgataagctc ctgttccagt gaaaacaggt attgtgcgtt
    20761 ggttcccagc ggaccgctcg ccgccgcaat caacggcgcg atgacctgag cgcgagtgtc
    20821 agattcatat tctggatgtc gcgggtccat aataaacaca atggcgttta ctgtgcgtcc
    20881 atcatcaaga tcaagctgac accaggttgg cagataacag ccggtaatca tctcgcgctt
    20941 ccacaacagg gttagttcct gctccagcgt ctcttctggt agtcgatagg cgacgcctgt
    21001 ggtgcgtccg ccctctttca gtgcaagcat ccgtcccggc tggtgcgcag tcccgcgccc
    21061 ggcggtcagg cgcaggcaaa acgcgcgatg ccatccaacc agtgtaccgg tgcataattc
    21121 ggtaaactcc agtgccggat tccacatcaa cgatccataa ccgaagatcc acaccggtcc
    21181 ctcattaggt cgacaagcca gcgtcgccgc cagcgaagcc gcccgttgtt ctgctgacca
    21241 taagagtgat tcttcaatgg caccaaatgc cgttttacaa tcggcattca tcaagaaatc
    21301 acgcgttatc accttacacc tccgccactg catacttccc tgcgacaact ttttggtgtc
    21361 ttccggacat ttttaccgct cattttgaaa ccactaacgg gacaatatgc aatccacgga
    21421 ccacttgcaa ggcaacaccg cacgaatagc tttaactaag ctaccggttt tacgattttt
    21481 tatgccattt atcatcatcc cctttggcat attcatgctt cacagcagcc caggccactt
    21541 tatgcgcggt ttcttcgcga ctggcgtcat cacgccgatc ttctttatct ttatattgat
    21601 cccatgcgct gttgaacgct tctttataga tatcctgggc atgagacggt agaacgtgct
    21661 ttacgctttc cggcagatcg cttttcgttt tatacggcat cgttgactcc ttttacaaag
    21721 ttgaacatta agtgtggtca acaatatcta ctcttgccaa aacataagaa ttttcatgtc
    21781 agactgttct taagaaacgc taaaacaaac attgtctaaa cgtaatactt atttttatgc
    21841 cccaatgagt aaaaaatcga cagcggggta aaaattcgta aaatgttacc gatatccaga
    21901 cactttctgt tatttttcct acctattttt tacattttcg ataaattcat ctgcaaaggg
    21961 ataaccataa tgtcaaatgc tcaagaggcg gtaaaaaccc gccacaagga gacttcgctt
    22021 attttcccgg ttctggcgct ggtagtgctg ttcctgtggg gaagcagcca gacactacca
    22081 gtggtcattg ccatcaatct tcttgcgctt attggtattt taagtagcgc ctttagtgtt
    22141 gtccgtcatg cggacgtatt agcccatcgc ctgggagaac cttacggttc gcttattctt
    22201 agcctttcag tggttattct tgaagtcagt ttgatttcag ctttaatggc aaccggcgac
    22261 gccgcgccaa cgctaatgcg tgatacgctc tattcaatca ttatgattgt taccggtggg
    22321 ctggttggct tttcattatt gttgggcggt cgtaagtttg ccacccaata tatgaatctg
    22381 tttggtatca agcagtattt aattgcgctg ttccccctgg cgataatcgt actggtattt
    22441 ccaatggctc tgcctgcggc gaatttttca accggtcagg cgttactggt agcattaatt
    22501 tctgcggcaa tgtatggcgt atttttgctg atccagacca aaacgcatca aagtttgttt
    22561 gtctacgagc acgaagatga cagtgatgac gacgacccgc accacggtaa accgtctgcc
    22621 catagcagcg tgtggcatgc tatctggttg attatccatc tgattgccgt tattgcagtc
    22681 accaaaatga atgccagccc gctggagaca ttgctcgaca gcatgaatgc ccctgtcgcc
    22741 tttactggct tcctggtggc tctgttgatt ctgtcgccgg aaggtttagg tgcgttaaaa
    22801 gcagtgttga acaaccaggt ccagcgcgca atgaatctgt tctttggttc agtgttagca
    22861 accatttcgc ttaccgtacc tgtcgtcacg ctaattgcct ttatgacggg taacgaatta
    22921 cagtttgcac ttggtgcgcc agaaatggtg gtgatggtgg cctctttagt gctgtgccat
    22981 atctccttct ccaccggacg tactaacgtg ctcaatggcg cagcgcatct ggcactgttt
    23041 gccgcctatt tgatgacgat atttgcctga cagttatatc tcctccgatg acggaaatcg
    23101 ggggagataa gataaattcc tgcttattgt atttcttcat actacctgga cagctcgtta
    23161 atcagaattg cgagcctgta ccacaacctg cacgaaacca ctgcaacagc tgttgaaaag
    23221 ccggaatgta ctctttattg caaacatttc agggatgaag tgaagatgaa ttgagacgta
    23281 gtatccacac caagccggaa ttatccggtg aggcgcaatg ttgcgggggc tttatccctg
    23341 gtggcattgg ttgctggaaa gagaaaaccc ccgcacgttg agaggtaaaa cctgacaaca
    23401 aaacgggggc taatcttgaa accagacact tcaagaatag ccgccttgtt gtcattacaa
    23461 caaggcggct atatgacgct cgcgcagttt gccatgactt tctggcacga cctggcagca
    23521 ccgatcctgg cggggattat taccgcagcg attgtcagct ggtggcgtaa ccggaagtaa
    23581 ggtgaaaacg ggcgtcaggc tgccgtgatg gcaatatgcg cccgaaaccg gccgcagggg
    23641 taacagcctg cggccatttt tgtctttatc tcttccacaa gtaaatattg gcaacccgag
    23701 ctggaagcat catccagagc actcatgaaa cgactgtaac agctgttgaa aaaccggaat
    23761 gcactcttca ttgcaaacat ttcagggatg aagtgaagat gaattgagtc gtagtatcca
    23821 caccaagccg gcgttatccg gtgaggcgca atgttgcggg ggcttaatcc ctggtggcat
    23881 tggttgctgg aaagagaaaa cccccgcacg ttgagaggta aaacctgaca acagaacggg
    23941 ggctaatctt gaaccatcaa caattcaaga atagccgcaa aatgttgtca ttacaacaag
    24001 gcggctatat gacgctcgcg aagtttgcca tgattttctg gcacgacctg gcagcaccga
    24061 tcctggcggg gattattacc gcagcgattg tcggctggtg gcgtaaccgg aagtaaggtg
    24121 aaaacgggcg tcaggctgcc gtgatggcaa tatgcgcccg aaaccggccg caggggtaac
    24181 agcctgcggc catttttgtc tttatctcct ccacaagtaa atattggcaa cccgagctgg
    24241 aagcatcatc cagagcactc atgaaacgac tgtaacagct gttgaaaaac cggaatgcac
    24301 tcttcattgc aaacatttca gggatgaagt gaagatgaat tgagacgtag tatccacatc
    24361 aagccggcgt tatccggtga ggcgcaatgt tgcgggggct ttatccctgg tggcattggt
    24421 tgctggaaag agaaaacccc cgcacgttga gaggtttaac ctgacaacag aacgggggct
    24481 aatcttgaac cagaacaatt caagaatagc cgcaaaatgt tgtcattaca acaaggcggc
    24541 tatatgacgc tcgcgcagtt tgccatgatt ttctggcacg acctggcagc accgatcctg
    24601 gcgggaatta ttaccgcagc gattgtcggc tggtggcgta accggaagta aggtgaaaac
    24661 gggcgtcagg ctgccgtgat ggcaatatgc gcccgaaacc ggccgcaggg gtaacagcct
    24721 gcggcctttt ttgtatctgg cacccatgaa aaaagtctta acgcagaacg ctaaggcttt
    24781 atttttcaag caaaaaagat tacttgctgg tatccagttc ttcgaaacct ttcaccagat
    24841 catcaatcgc tttcatctgc ttgaggaacg gttccagttt agccagcggc agcgcggatg
    24901 gaccatcaca tttcgcatgt tccggatccg gatgcgcttc aataaacagc cccgccagac
    24961 ctaccgccat accggctcgt gccagctcag ccacctgagc acgacgacca ccggaagctg
    25021 cgccaaacgg atcgcggcat tgcagtgcgt gggtcacgtc gaaaatcacc ggcgagttac
    25081 cagacacttt cttcataatg ctgaagccca gcatatcgac aaccaggttg tcatagccga
    25141 agttagcacc gcgatcgcaa agaatcactt tttcgttgcc gccttctttg aatttatcaa
    25201 cgatattacc catctgtccc gggctgacaa actgtggttt cttgacgtta attaccgcac
    25261 cggttttcgc catggcttca accaggtcag tctggcgagc aagaaacgcc ggcaactgaa
    25321 tcacatccac gacatcagca acgggctgtg cctgacttgg ttcgtgaacg tcggtgataa
    25381 ttttcacgcc aaaagtctgc ttcaactcct ggaagatttt catcccttct tccaggcccg
    25441 gtccacgata agagtggatg gaggagcggt tggctttgtc aaaagaggct ttgaacacgt
    25501 aagggatacc cagtttctgg gtcacggtta cgtagtgctc gcaaatgcgc atcgccagat
    25561 cgcgagattc cagcacgttc ataccaccaa acagtacgaa cggcaggtca tttgctacgt
    25621 tgatgtcgcc aatgctaacc actttttgtt tcataggatc gccttaatct tgagtaaaat
    25681 gtcgattaat taatgcagca caatatgttt atgcgcgatg ttatttattt gcgcacggat
    25741 catttcgctg atcgggtctt ccggacactg ttcaacgaaa taacttaaat cgttcaacgc
    25801 aacgtgttcg caatccagtt gcgcataaat caacccgcga tcgcgaattt catagggatc
    25861 ttccgggttg aattgtaata aagcttcgct ggtgcgtaac gccagctcca tctgattttc
    25921 ttccatcaac gaggctttga gtgtatccaa caatttgcgg attacctcaa tgttatcagc
    25981 ttcatcaagg tcttcataaa acagttccgc cgacgggctg atatttccct ttaaccatac
    26041 gtccagcata tgttcgctta acgattcacc gttaaaagga ttaatcagcc aaatttcgcc
    26101 atccggacat tcaatgcgca atatcagctg cgtagggaaa atcaccggca gcagcggcaa
    26161 atcgagacga ttcgcgaccc ataataaaac cgcacctaat gataccgcac tgcctgtcga
    26221 ttctttaaca cctggtccag ccataatgca tcggaaagac gataaacgcc gcgtgaggct
    26281 ttaaatcccc agtcgccgta aaacagcgcg atcagttttt ccagctgctc ttctaaaggc
    26341 agaagctggc tgatttcttc cttcgccaga ctaacgagac gctccagttc gtcgtaaaca
    26401 tcttgcgagg gaaaatcgcg gcggattgct tcgcaagcca ggatcatgcc ttcgcacaat
    26461 ggcgctttat taaattcgaa atcagctaac gatctcatga cttaccccag taacggtact
    26521 tttgtggtgg cgagtttaat gatgatgtac agcaccacca acgccagcgg gaaggcaata
    26581 atgcgcgcct gctgactgtg catacgacga taatcgagtg caataaaacc caaaacgata
    26641 taaatgataa ctccaaacag cttttcagtc agccactgtg cctgcccgga aaatggcagg
    26701 atgtgcgctt tagccatcaa cgcaatgccg cttaacaata acaccgtatc gacaaccggc
    26761 ggcacaatgc gcgtccagcg agcaaatgcc tgtggatgct tctgataacg tagccagaaa
    26821 cgtaaggtta atagcccaac agaaagcgcg atactaataa gatgaacact aagcagtgta
    26881 gaaaaacttg tcattgataa tagcggccga gcgttacgcg ctcgttatca ccatagtcac
    26941 ggcaggtttc gacgtcatga taccccgcga ggataaatgc ttgtcgcacc gcttcgccct
    27001 gctgccagcc atgttccaga agcagaaagc cgccggatac cagcgcgtta cgcgactgtt
    27061 cgatgatatg cacgatgtct gccattccac tgtctgccgc aaccagcgca gtgagcggct
    27121 caaagcggac atcgccttgt tgaagatgtg ggtcctgctc gtcaatatac ggcggattgc
    27181 tgacaatcat cgcaaactgc tgcccggcta gcgcgctaaa ccagtcgctt tgcagaatgt
    27241 ggatattttt gatcgccaga tgctgggcat tacgttgtgc cagggagaca gcatcaggca
    27301 tacgatctac agcgataatt tcgcagtccg ggcgctcgct agccagcgca agcgcaatcg
    27361 ccccggtacc cgtcccgaga tcgagaatac ggcaaggttg ttcaggcaac cgcgccagtg
    27421 cctgctccac cagacactcc gtatccgggc gcggaattaa ggtcgctggc gaaacaaata
    27481 acggcaacga ccagaattct cgcaccccgg ttaaatgagc aatgggttca ccatcgcgac
    27541 gacgtgtcag tagcgcatca agttgctgac attgttcgtc agtcagctgc gtttcaccaa
    27601 aggccagaat aaaagtccgc cccctgccag tgacatactc caataagatt tcagcgtcac
    27661 ggcgtgggct ttcgctcgcc tgaagttggc ttattgcttc acgtaaccag tattgatatt
    27721 ccattattcc tgctcggaca acgccgccag ttggtcggcc tgatgttcct ggataatcgg
    27781 ttcaatcagc atatccagct taccttccat cacttcatcc agacggtaga gcgtcaggtt
    27841 gatgcggtga tcggtaacgc gcccctgcgg gaagttgtaa gtacggttac ggtcgctgcg
    27901 atcgccactc cccagcaggt tacgacgggt agacgcttcg gcctgttgcc gttttgccat
    27961 ttctgcagcg tggatacgag caccaagtac agaaagtgct ttagctttgt ttttatgttg
    28021 tgaacgttcg tcctgacatt caacaacaat cccggtcggc aagtgagtaa tacgaattgc
    28081 cgaatcggtg gtgttaacgt gctgaccacc cgcccctgac gagcggaaag tatcaatgcg
    28141 taaatctgct gggttgatgt ccggcagttc tgcgtcaggc agttctggca ttaccgcaac
    28201 ggtacaagca gaagtatgaa tacgaccctg cgattccgta gcaggaacac gctgcacgcg
    28261 atgaccgccg gattcaaatt tcagacgacc atacacacca tcaccgctaa ttttggcgat
    28321 gatctcttta taaccaccat gttcaccctc gctggcgctc atgatttcta cccgccagcg
    28381 gcgggcttcg gcataacggc tgtacatacg gaacagatcg cccgcgaaca gcgccgcttc
    28441 gtcgccgccg gttccggctc ggacttcgag gaaggcgtta cgttcgtcat caggatcttt
    28501 tggcagtaac agaacctgta attgctgttc cagttgctcg cttttttctt tagcttcgcg
    28561 cagttcatcc tgcgccatct cacgcatttc aggatcatcg agcatcatct gtgcggtttc
    28621 gatatcttcc tgaacctgtt gccagtcggt aaaacagcgc gaaacatcac ttaactgcgc
    28681 atattcgcgt gataatgcgc gaaaacgttc ctggtcggcg atagtttgcg cgtcacccag
    28741 caacgcctga acttcttcat ggcgttcatg cagggcttcc agtttggcaa cgatagaagg
    28801 cttcataggc gtaaatgcac cctgtaaaaa aagaaaatga tgtactgcta ctccagcccg
    28861 aggctgtcgc gcagaatatt caggcgttcg ttatccccgt cacgggcggc ctgttgaagt
    28921 gatttcgttg gcgcatggat caagcggtta gtcagtttcc atgccagatc ctgcataatg
    28981 gcttgcgcgt cgccgccctg ctcaagggcc gctaacgctt tggcggttaa ctcatcgcga
    29041 acgtgctctg cctggctgcg atactcgcga atggtttcgc tggcgctttg tgctcgcagc
    29101 cacgccataa attcgctggt ttcctgagcg acaatagttt ccgcctcaac cgctgcggct
    29161 ttacgctgcg ccaggttgtg cgaaatgatg ctttgcagat cgtccacact ataaagataa
    29221 gcattcgcca gtttgccaac ttccggctca acatcgcgcg gaacggcaat atccaccaac
    29281 agcattggtt ggttacgacg actttttaat gcgcgctcca ccatgccttt cccgataatc
    29341 ggtaacgggc tggcggtgga actgatgatg atatcggctt cgcgcagacg ttcgtcgatc
    29401 tcactcaggg caatcacttc cgcgccaact tcatctgcca gaatttgggc acgttcgcga
    29461 gtgcggttgg cgataatcat cttctgtact ttatgttcgc gcagatgacg cgctaccagc
    29521 tcgatggttt cgcccgcgcc taccagcaac actgtgaccg tagagagcga ttcaaagatc
    29581 tgccgcgcca gcgtacaagc cgcaaaagcg acagacacag cactggcacc gatatctgtt
    29641 tcagtgcgaa cgcgtttcgc tacagagaaa gatttctgga acatgcgttc cagttcgctg
    29701 gccttcatat gacctttttg cgaatcggca aacgcttttt taacctgacc gaggatctgc
    29761 ggctccccaa gaaccagtga atccaggccg ctggcaacac gcattaaatg gctaaccgcg
    29821 tcgttatcct gatgccagta gaggctttta cgcagatctt cttcattaag attgtgataa
    29881 tcgcaaagcc agcggattaa cgcctcttgc aggttatcct gctcttcaac gctaagataa
    29941 agttccgtgc ggttgcacgt cgacagcacc acgccgccct gcaccatcgg ctgcgcaagc
    30001 aggctgtcaa gcgcctgatc gagcttatcc ggcgaaaacg atacacgttc tcgcagcgat
    30061 acaggtgccg ttttatggtt gataccgagt gctaaaaggg tcatgtctgc gggaaataat
    30121 accaacgttg atatggttag tctgcttgca tcatacagga tgcgtaagat caataaaaga
    30181 gagcgccccc ttttggagta attgccgaaa gccgttagat tctggcaatt aagacaactt
    30241 gaacatagac gatagcggac ggtaacgcta gcattaaggg ttataactgc aacgtatctc
    30301 aaggacttgt catcactatg ccccagcccg attttcgtct tatccgcctg ctaccgctgg
    30361 ctgctcttgt gctcactgcc tgttccgtta ccacgcccaa aggtcctggc aaaagcccgg
    30421 attcgccaca atggcgtcag catcagcaag acgtgcgcaa tcttaatcag tatcagactc
    30481 gcggcgcgtt cgcttatatt tctgaccaac aaaaagtgta cgcccgcttt ttctggcagc
    30541 aaaccggcca ggatcgctac cgtctgctgc tcactaaccc attgggcagc acggaactgg
    30601 agctgaatgc tcaaccgggt aacgtgcagt tagtcgacaa taaaggtcag cgttataccg
    30661 ccgatgacgc cgaagagatg attggcaaat tgaccggaat gccaattccg ctcaacagct
    30721 tgcgccaatg gattttaggt ttaccgggtg atgcaaccga ctacaaactg gacgaccagt
    30781 accgcctgag cgaaattacc tacagccaga atggcaaaaa ctggaaggtt gtttatggtg
    30841 gttatgacac caaaacgcaa cctgcgatgc cagccaatat ggaactcacc gacggtggtc
    30901 aacgcatcaa gttaaaaatg gataactgga tagtgaaata atgcggacac agtggccctc
    30961 tccggcaaaa cttaatctgt ttttatacat taccggtcag cgtgcggatg gttaccacac
    31021 gctgcaaacg ctgtttcagt ttcttgatta cggcgacacc atcagcattg agcttcgtga
    31081 cgatggggat attcgtctgt taacgcccgt tgaaggcgtg gaacatgaag ataacctgat
    31141 cgttcgcgca gcgcggttgt tgatgaaaac tgcggcagac agcgggcgtc ttccgacggg
    31201 aagcggtgcg aatatcagca ttgacaagcg tttgccgatg ggcggcggtc tcggcggtgg
    31261 ttcatccaat gccgcgacgg tcctggtggc attaaatcat ctctggcaat gcgggctaag
    31321 catggatgag ctggcggaaa tggggctgac gctgggcgca gatgttcctg tctttgttcg
    31381 ggggcatgcc gcgtttgccg aaggcgttgg tgaaatacta acgccggtgg atccgccaga
    31441 gaagtggtat ctggtggcgc accctggtgt aagtattccg acaccagtga tttttaaaga
    31501 tcctgaactc ccgcgcaata cgccaaaaag gtcaatagaa acgttgctaa aatgtgaatt
    31561 cagcaatgat tgcgaggtta tcgcaagaaa acgttttcgc gaggttgatg cggtgctttc
    31621 ctggctgtta gaatacgccc cgtcgcgcct gactgggaca ggggcctgtg tctttgctga
    31681 atttgataca gagtctgaag cccgccaggt gctagagcaa gccccggaat ggctcaatgg
    31741 ctttgtggcg aaaggtgtta atctttcccc attgcacaga gccatgcttt aagccgggca
    31801 agctgagttt cggtgacaac gtcaccttgt tccagacgtt gcatcgcgct ctttaataca
    31861 ccgcctggaa aggatcatgc ctggcccgca cagttttcgg cagattcttt ccaccaatgg
    31921 acgcatgcct gaggttcttc tcgtgcctga tatgaagctt tttgctggta acgccacccc
    31981 ggaactagca caacgtattg ccaaccgcct gtacacttca ctcggcgacg ccgctgtagg
    32041 tcgctttagc gacggcgaag tcagtgtaca aatcaacgaa aatgtacgcg gtggtgatat
    32101 tttcatcatc cagtccactt gtgcccctac taacgacaac ctgatggaat tagtcgttat
    32161 ggttgatgcc ctgcgtcgtg cttccgcagg tcgtatcacc gctgttatcc cctactttgg
    32221 ctatgcgcgc caggaccgtc gcgtccgttc cgctcgtgta ccaatcactg cgaaagtggt
    32281 tgcagacttc ctctccagcg tcggtgttga ccgtgtgctg acagtggatc tgcacgctga
    32341 acagattcag ggtttcttcg acgttccggt tgataacgta tttggtagcc cgatcctgct
    32401 ggaagacatg ctgcagctga atctggataa cccaattgtg gtttctccgg acatcggcgg
    32461 cgttgtgcgt gcccgcgcta tcgctaagct gctgaacgat accgatatgg caatcatcga
    32521 caaacgtcgt ccgcgtgcga acgtttccca ggtgatgcat atcatcggtg acgttgcagg
    32581 tcgtgactgc gtactggtcg atgatatgat cgacactggc ggtacgctgt gtaaagctgc
    32641 tgaagctctg aaagaacgtg gtgctaaacg tgtatttgcg tacgcgactc acccgatctt
    32701 ctctggcaac gcggcgaaca acctgcgtaa ctctgtaatt gatgaagtcg ttgtctgcga
    32761 taccattccg ctgagcgatg aaatcaaatc actgccgaac gtgcgtactc tgaccctgtc
    32821 aggtatgctg gccgaagcga ttcgtcgtat cagcaacgaa gaatcgatct ctgccatgtt
    32881 cgaacactaa tcgaacccgc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc
    32941 catttgtatg acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg
    33001 atgaaacatt attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat
    33061 cgacgcttgc tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg
    33121 gataaccgtc gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt
    33181 ggcaccccag tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctgacggg
    33241 tgggtcacgc tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt
    33301 gtcgcaacag tttggactgg caggactgct ggttgcgacc ttgctgtcgg ggatcttttt
    33361 gattctgatg ggtctggcac gctttggtcg cctgattgag tatattccgg tttccgtcac
    33421 cttaggtttc acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct
    33481 cggtctgcaa atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat
    33541 ggcgctgccg accattaatg tgggtgatgc tgccattggc attgtgacgc taggtattct
    33601 tgttttttgg ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg
    33661 ttgcgcggtg atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca
    33721 attccactac gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca
    33781 actggtgctg ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg
    33841 cacactgctg cctgcggcat tctcaatggc aatgctcggc gcaatcgaat ctctgctctg
    33901 cgccgtggta ctggatggta tgaccgggac gaaacacaag gcgaacagcg aactggttgg
    33961 acagggactg gggaatatta tcgctccgtt ctttggtggt attaccgcta cagctgccat
    34021 cgcgcgttct gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca
    34081 ctctattctg gttattcttg ccctgctggt actggcaccg ctgctctcct ggctgccgct
    34141 ttccgctatg gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcataaagt
    34201 ggtcgacttg ctgcgtcatg caccgaaaga tgacatcatt gtcatgctgc tgtgcatgtc
    34261 gctgaccgtg ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct
    34321 gctgtttatg cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc
    34381 agacgatgtt ctggtactgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt
    34441 gttcacggac ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc
    34501 cgttccggta cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc
    34561 cgaaggatgt gaactgcgcg tgtgcaacgt ggaattccag ccactgcgca ctatggctcg
    34621 cgcaggcatt caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat
    34681 ggcggattta taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca
    34741 ttatttgtta gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa
    34801 cccaccagta acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga
    34861 gaatggcacc gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa
    34921 actgtggtaa ctggttgagg attgaatacc ccacgccgac caccattacc accagcccca
    34981 accccatgag cacgttaccg agtaacgaag cgtttttgcg tttcatatgt cacctccgga
    35041 actttctggg ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc
    35101 ctgtgggtta tgtgcgggcg tgatcacaat tacaaccctt atttcaacaa aactttacaa
    35161 ataaacgcct gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg
    35221 catttgatac gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat
    35281 gtacacactc aggacaaaaa acgtgacgat taaattgatt gtcggcctgg cgaaccccgg
    35341 tgctgaatac gccgcaacgc gacataatgc tggtgcctgg ttcgttgact tactggcaga
    35401 gcgtttgcgc gctccgctgc gcgaagaggc taaattcttt ggttatactt cgcgagtcac
    35461 tcttggaggc gaagatgtcc gcctgttagt cccgactaca tttatgaatc tcagcggcaa
    35521 agccgttgcg gcgatggcca gttttttccg cattaatccg gacgaaattc tggtggccca
    35581 cgacgaactg gatctgcctc ctggcgtcgc caaatttaaa ttgggcggtg gccatggtgg
    35641 tcacaatgga ctgaaagaca tcatcagtaa attgggtaat aaccctaact ttcaccgttt
    35701 acgcatcgga atcggtcatc ctggcgataa aaataaagtt gtcggttttg tgttaggcaa
    35761 accgcctgtt agtgaacaga agttaattga tgaagccatc gacgaagcgg cgcgttgtac
    35821 tgaaatgtgg tttacagatg gcttgaccaa agcaacgaac cgattgcacg cctttaaagc
    35881 gcaataagtc gttgtctgcg gcatttttgc cgagtgccgt gtataatagg caaagttatt
    35941 tccatttctg caatctgtta gcaataacag gttgattatt aagatattaa ggtgatttaa
    36001 atcatgggat tcaaatgcgg tatcgtcggt ttgcccaacg tcgggaaatc taccctgttc
    36061 aacgcgctga ccaaagccgg tattgaagcg gccaactttc cattctgcac cattgagccg
    36121 aacacaggcg tcgtaccaat gcctgaccct cgcctggatc aactggctga aatcgtaaaa
    36181 ccgcaacgta cgcttcccac gaccatggaa tttgtcgata tcgccggtct ggtaaaaggc
    36241 gcatcgaaag gcgaaggtct gggtaaccag ttcctgacca acatccgtga aaccgaagcg
    36301 atcggtcacg ttgttcgctg ctttgaaaat gacaacatca ttcacgtttc cggcaaagtt
    36361 aacccggctg acgatattga agttatcaac accgaactgg cgctggcaga cctcgacacc
    36421 tgcgaacgtg cgattcatcg cgtacagaag aaagccaaag gtggcgataa agacgcgaaa
    36481 gctgagctgg cggtcctaga aaaatgcctg ccccagctgg aaaacgcagg tatgctgcgc
    36541 gcgctggatt taagcgctga agagaaagcg gctattcgtt acctgagctt cctgacgctg
    36601 aaaccaacaa tgtacatcgc caacgtcaac gaagacggtt ttgaaaacaa cccatatctt
    36661 gaccaggtgc gtgaaatcgc ggcgaaagaa ggttctgttg tggttccggt ttgtgctgct
    36721 gttgaagcag acattgccga actggacgac gaagaacgtg acgagtttat gcaggagctt
    36781 gggctggaag aaccgggcct gaaccgtgtg atccgtgtcg gttataagct gctgaacctg
    36841 caaacttact tcaccgctgg ggtgaaagaa gtgcgtgcat ggaccattcc ggttggagca
    36901 accgcgccgc aggcagcggg caaaatccat actgattttg aaaaaggctt tatccgtgca
    36961 caaaccatct cgtttgaaga tttcatcact tacaaaggtg aacaaggcgc gaaagaagca
    37021 ggcaaaatgc gtgcagaagg taaagattac atcgttaaag atggcgatgt gatgaacttc
    37081 cttttcaacg tctaattaat ttattgtctc atgaggtttc gctaaatctc atgaagagcg
    37141 caaaagtata taaaatccac gcaattgcgt ggatttttca tttcaccgag tcctaatcat
    37201 tcttgtaaca tcctacactt ttgtgcttat tgcctacagt gaaccacgag aataattaaa
    37261 gacatcagtg tcagatgacg gatatctcat tagtagtgat ataacgtaag tttttgttca
    37321 actacacatc acccccttca cagctgatca atattgatca cgctccaata tgctggcgag
    37381 aaaaggactt acctgaaggg taatatcccg cagataaagt ccccgaataa cgtgattacg
    37441 tgcgatcctg gcaagatgaa aatgataacc agtagcacaa taacaacgat aggccagaaa
    37501 acccattgac caaaaatgaa tatcgatgtt ttttgctttt tcttattagt aatgcaaaaa
    37561 tggcgcatat tgtttttgtt atgcagatac atcaagcgaa attcccataa ctaattagga
    37621 ttattacaaa aaatcttcac caaatttttt aaccgctgtt tgttgtaata tttttgccat
    37681 atttatagta acgaattgga ataagtaacg acaacttagg gaataactcg atataaactc
    37741 acctttgtaa gggggatgtt aaatatatat taattcctgt gttcctgatt ttgcccggta
    37801 atagagaatt attctctgag aagctcatca tgattgcacc atacaaatat catgatgata
    37861 agaggatatt attcattgat ttaaacaaaa tgacaaaatc catagaccca tagtctgggt
    37921 actatatagg agtggtatgg gcatcaaaca acacaatggg aataccaaag cagatcgtct
    37981 cactgaat
//
LOCUS       DAEEKQ010000024        36331 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.024, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000024 DAEEKQ010000000
VERSION     DAEEKQ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.024"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            417..1865
                     /gene="uacT"
                     /locus_tag="KLK57_001175"
     CDS             417..1865
                     /gene="uacT"
                     /locus_tag="KLK57_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HBE0891603.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPFIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFLHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            1867..1992
                     /locus_tag="KLK57_001176"
     CDS             1867..1992
                     /locus_tag="KLK57_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891604.1"
                     /translation="MNFLMRVIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(1989..2060)
                     /locus_tag="KLK57_001177"
     CDS             complement(1989..2060)
                     /locus_tag="KLK57_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891605.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            2115..2663
                     /gene="idi"
                     /locus_tag="KLK57_001178"
     CDS             2115..2663
                     /gene="idi"
                     /locus_tag="KLK57_001178"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HBE0891606.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTRLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESNEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPNGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQAANSEARKLLSAFAQHN"
     gene            complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="KLK57_001179"
     CDS             complement(2706..4223)
                     /gene="lysS"
                     /locus_tag="KLK57_001179"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBE0891607.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(4233..5331)
                     /gene="prfB"
                     /locus_tag="KLK57_001180"
     CDS             complement(join(4233..5255,5257..5331))
                     /gene="prfB"
                     /locus_tag="KLK57_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HBE0891608.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="KLK57_001181"
     CDS             complement(5422..7155)
                     /gene="recJ"
                     /locus_tag="KLK57_001181"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HBE0891609.1"
                     /translation="MKQQIQLRRRAVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEADKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="KLK57_001182"
     CDS             complement(7161..7871)
                     /gene="dsbC"
                     /locus_tag="KLK57_001182"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HBE0891610.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     ALQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="KLK57_001183"
     CDS             complement(7896..8792)
                     /gene="xerD"
                     /locus_tag="KLK57_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HBE0891611.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            8904..9425
                     /gene="fldB"
                     /locus_tag="KLK57_001184"
     CDS             8904..9425
                     /gene="fldB"
                     /locus_tag="KLK57_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HBE0891612.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            complement(9465..9872)
                     /locus_tag="KLK57_001185"
     CDS             complement(9465..9872)
                     /locus_tag="KLK57_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin CptA"
                     /protein_id="HBE0891613.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="KLK57_001186"
     CDS             complement(9853..10119)
                     /gene="sdhE"
                     /locus_tag="KLK57_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HBE0891614.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            10362..11342
                     /gene="ygfZ"
                     /locus_tag="KLK57_001187"
     CDS             10362..11342
                     /gene="ygfZ"
                     /locus_tag="KLK57_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HBE0891615.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDEHVLLGVAGFQARAALANIFSELPSKEKQVVKEGATTLLWFEYPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            complement(11538..12197)
                     /locus_tag="KLK57_001188"
     CDS             complement(11538..12197)
                     /locus_tag="KLK57_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HBE0891616.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(12361..12672)
                     /locus_tag="KLK57_001189"
     CDS             complement(12361..12672)
                     /locus_tag="KLK57_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HBE0891617.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            12717..14150
                     /gene="bglA"
                     /locus_tag="KLK57_001190"
     CDS             12717..14150
                     /gene="bglA"
                     /locus_tag="KLK57_001190"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HBE0891618.1"
                     /translation="MKKLTLQKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVITSNGEKL"
     gene            complement(14207..14950)
                     /locus_tag="KLK57_001191"
     CDS             complement(14207..14950)
                     /locus_tag="KLK57_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0891619.1"
                     /translation="MAIALVTDGSRGIGRATALLLAKEEYTVAVNYQQNLHAAQEVVN
                     LITQAGGKAFVLQADISDENQVIAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNMTGYFLCCREAVKRMALKNGGSGGAIVNVFSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGVRVNCVRPGFIFTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="KLK57_001192"
     CDS             complement(15216..18089)
                     /gene="gcvP"
                     /locus_tag="KLK57_001192"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HBE0891620.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAAVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="KLK57_001193"
     CDS             complement(18208..18597)
                     /gene="gcvH"
                     /locus_tag="KLK57_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HBE0891621.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="KLK57_001194"
     CDS             complement(18621..19715)
                     /gene="gcvT"
                     /locus_tag="KLK57_001194"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HBE0891622.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVLEGI
                     GETAIVQIRNREMSVKVTKPVFVRNGKAVA"
     gene            20005..20088
                     /locus_tag="KLK57_001195"
     CDS             20005..20088
                     /locus_tag="KLK57_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891623.1"
                     /translation="MSKNTKSKNNDIRKYNAKTEVKLVYFK"
     gene            complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="KLK57_001196"
                     /gene_synonym="visC"
     CDS             complement(20162..21364)
                     /gene="ubiI"
                     /locus_tag="KLK57_001196"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HBE0891624.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="KLK57_001197"
                     /gene_synonym="visB"
     CDS             complement(21387..22565)
                     /gene="ubiH"
                     /locus_tag="KLK57_001197"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HBE0891625.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="KLK57_001198"
     CDS             complement(22562..23887)
                     /gene="pepP"
                     /locus_tag="KLK57_001198"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HBE0891626.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNELEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIMNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="KLK57_001199"
     CDS             complement(23913..24491)
                     /gene="ygfB"
                     /locus_tag="KLK57_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HBE0891627.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            24659..24988
                     /gene="zapA"
                     /locus_tag="KLK57_001200"
     CDS             24659..24988
                     /gene="zapA"
                     /locus_tag="KLK57_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HBE0891628.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25030..25213
                     /gene="ssrS"
                     /locus_tag="KLK57_001201"
     ncRNA           25030..25213
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="KLK57_001201"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            25234..25836
                     /locus_tag="KLK57_001202"
     CDS             25234..25836
                     /locus_tag="KLK57_001202"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HBE0891629.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(25937..25996)
                     /locus_tag="KLK57_001203"
     CDS             complement(25937..25996)
                     /locus_tag="KLK57_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBE0891630.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="KLK57_001204"
     CDS             complement(26328..26387)
                     /gene="ibsC"
                     /locus_tag="KLK57_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HBE0891631.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(26616..27848)
                     /gene="serA"
                     /locus_tag="KLK57_001205"
     CDS             complement(26616..27848)
                     /gene="serA"
                     /locus_tag="KLK57_001205"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HBE0891632.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="KLK57_001206"
     CDS             complement(28104..28763)
                     /gene="rpiA"
                     /locus_tag="KLK57_001206"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HBE0891633.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            29193..30086
                     /gene="argP"
                     /locus_tag="KLK57_001207"
     CDS             29193..30086
                     /gene="argP"
                     /locus_tag="KLK57_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HBE0891634.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            30289..32433
                     /gene="scpA"
                     /locus_tag="KLK57_001208"
     CDS             30289..32433
                     /gene="scpA"
                     /locus_tag="KLK57_001208"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HBE0891635.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKQLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAADHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            32426..33421
                     /gene="meaB"
                     /locus_tag="KLK57_001209"
     CDS             32426..33421
                     /gene="meaB"
                     /locus_tag="KLK57_001209"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HBE0891636.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            33432..34217
                     /gene="scpB"
                     /locus_tag="KLK57_001210"
     CDS             33432..34217
                     /gene="scpB"
                     /locus_tag="KLK57_001210"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HBE0891637.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMMPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            34241..35719
                     /gene="scpC"
                     /locus_tag="KLK57_001211"
     CDS             34241..35719
                     /gene="scpC"
                     /locus_tag="KLK57_001211"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HBE0891638.1"
                     /translation="METQWTRMTADEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNHYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(35716..>36331)
                     /locus_tag="KLK57_001212"
     CDS             complement(35716..>36331)
                     /locus_tag="KLK57_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0891639.1"
                     /translation="IVIGIDNTYPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLF
                     DRQLDFIISPQHVSARVQELENLTISELPPLRLGFLVSRRYEERQEQELLQELPWLQM
                     RFQNRANFEAMIDANMRPCGINPTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNP
                     ATLKYFDAPHRPMYMQEYLYSIRNHRYTATMLQHIAEDRDGTNH"
BASE COUNT         8703 a         9741 c         9170 g         8717 t
ORIGIN      
        1 tgagttatgt cttctcaaat ttcgcgatgc aataaacggg catatattag gtagcatgac
       61 cgttttaatt ataattttgc gactgagttc aaattattgc ccctcaatct atgccgcttt
      121 cattatttca acattgccga tcaaaagagc gctatccaga taaaaacgat taaatgcgag
      181 agtgcgacat gccagaatga ttaatgaaat atcaatatga gaaaattaca gataattcca
      241 ttgtcaggtt ctcttgtcat tttcgtggtt taagcacgat tatgtgattg cctccgcaat
      301 tcctgtctct aactcccctt cctcgcaaaa actggcactc cacgagcatg tgtttagaca
      361 gtttcattaa cgtaaacggt tgctttttac tctggcgggc gaaaggagaa acactgatga
      421 gcgccataga ttcccaactt ccctcatctt ctgggcaaga ccgcccaact gatgaggttg
      481 accgcatatt atcaccagga aagctgatca tactcggtct gcaacacgtc cttgtcatgt
      541 acgcaggtgc agtcgctgtt cctcttatga ttggtgaccg actgggcctc tcaaaagatg
      601 ctattgcgat gctcattagc tcggatctct tttgctgcgg gatcgtcaca ttattgcaat
      661 gtatcggtat cggccgcttt atggggatcc gcctgccggt gattatgtcg gtgacctttg
      721 ctgctgtaac accaatgata gccattggga tgaacccgga tatcggcctg ctggggatat
      781 ttggtgccac tatcgccgcg ggttttatca ccacattatt agcgccattt attggtcgct
      841 tgatgccttt attcccgcca ctggttaccg gtgtggttat tacttctatt gggcttagca
      901 tcattcaggt gggtattgac tgggccgccg gaggtaaagg gaatccgcaa tatggtaatc
      961 ccgtttattt aggtatctcc tttgctgtct taatttttat cttgctcatt actcgctatg
     1021 cgaaaggatt tatgtccaac gtcgccgtat tactggggat tgtatttggc tttttacttt
     1081 cgtggatgat gaatgaagtc aatttatccg ggctacatga tgcttcatgg tttgcgattg
     1141 tcacgccgat gtcatttggt atgccgattt tcgatcccgt ttccattctg accatgactg
     1201 ccgtgttaat catcgtgttt atcgagtcga tggggatgtt cctggcactg ggtgaaatag
     1261 tcggtcgtaa actctcttca cacgatatta ttcgcgggct gcgtgtcgat ggcgtaggga
     1321 caatgatagg cggaacgttt aacagcttcc tccacacgtc attttcacaa aacgttggcc
     1381 tggttagcgt gacgcgcgtt catagccgct gggtgtgtat ttcttcggga attatattaa
     1441 tcctgtttgg catggtgcca aaaatggcgg tgctggtcgc ctccattccg caatttgtgc
     1501 tgggcggtgc tgggctggtg atgttcggca tggtactggc gacagggatt cgaattctgt
     1561 cgcgctgtaa ctacaccacc aaccgttaca acctctatat tgtggcgatc agtctcggcg
     1621 ttggcatgac tccgacgctc tctcacgatt tcttttctaa gttaccggcc gtactgcaac
     1681 cgttgctgca tagcggcatt atgctcgcaa cccttagcgc cgttgtgctg aatgtcttct
     1741 ttaatggcta ccagcatcat gctgacctgg tgaaggaatc cgtctctgat aaagatttaa
     1801 aagtcaggac agtacgtatg tggcttctga tgcgcaagct gaagaaaaat gagcatggag
     1861 aataatatga attttttaat gcgcgttata ttcagtctgc tgttgctttt tactctctct
     1921 attcctgtca tttctgactg tgttgcaatg gccattgaaa gtcgcttcaa atatatgatg
     1981 ctactttttt aaatggtttt tacctgtcgg catccgctca aaacgggcgg ttgtcgataa
     2041 acgctcactt ggttaatcat ttcactcttc aattatctat aatgatgagt gatcagaatt
     2101 acatgtgaga aattatgcaa acggaacacg tcattttatt gaatgcacag ggagttccca
     2161 cgggtacgct ggaaaagtat gccgcacaca cggcagacac ccgcttacat ctcgcgttct
     2221 ccagttggct gtttaatgcc aaaggacaat tattagttac ccgccgcgca ctgagcaaaa
     2281 aagcgtggcc tggcgtgtgg actaactcgg tttgtgggca cccacaactg ggagaaagca
     2341 acgaagacgc ggtgatccgc cgttgccgtt atgagcttgg cgtggaaatt acgcctcctg
     2401 aatctatcta tcctgacttt cgctatcgcg ccaccgatcc gaatggcatt gtggaaaatg
     2461 aagtgtgtcc ggtatttgcc gcacgcacca ccagtgcgtt acagatcaac gatgatgaag
     2521 tgatggatta tcaatggtgt gatttagcag atgtattaca cggtattgat gccacaccgt
     2581 gggcgttcag tccgtggatg gtgatgcagg cagccaatag tgaagcaaga aaattgttgt
     2641 ctgctttcgc gcagcacaat taaaaaaccc cgacatttgc cggggttgtg agcataacgt
     2701 aatgcttatt ttaccggacg catcgccggg aacagaataa cgtcgcggat ggtatggctg
     2761 ttggtgaaca gcattaccat acggtcgatg ccaatcccca gacctgctgt cggcggtaag
     2821 ccatgttcca gtgcggtaac gtaatcttca tcgtagaaca tcgcttcgtc gtcacctgcg
     2881 tctttcgcgg caacctgatc caggaagcgt tgcgcctgat cttccgcgtc attcagctcg
     2941 ctaaagccgt taccgatttc acgaccaccg atgaagaact caaagcggtc tgtgatttcc
     3001 gggttaatgt cgttacgacg cgccagcgga gaaacttctg ccggatattc agtaatgaag
     3061 gtcggctgaa tcagatgtgc ttctgccact tcttcgaaga tctcggtaac gatacggccc
     3121 agaccccagc tcttctcaac gtggataccg atagattcag caatcgcttt cgcagagtcg
     3181 aagttatcca gatccgccat gtcggtttcc gggcgatatt tcttgatcgc ttcacgcatg
     3241 gtcagttttt cgaacggttt accgaagtcc agcgtcacgt cgccgtaggt cacttccgtc
     3301 ttaccgagaa tatcctgtgc cagagtacgg aacagcgatt cggtcagctc gatcagatct
     3361 ttgtaatctg cgtaagccat gtagagttcc atcatggtga actctgggtt atgacgtacg
     3421 gaaatacctt cgttacggaa gttacggttg atttcgaata cacgctcgaa gccaccgacc
     3481 accagacgct tgaggtacag ttccggcgcg atacgcaggt acatgtcgag atccagcgcg
     3541 ttatggtggg tgataaacgg acgcgcagcg gcaccgcctg ggatcacctg catcatcggc
     3601 gtttcaactt ccataaagcc gcggttcacc atgaactggc gaataccaga gaggatctgc
     3661 gagcgtactt taaaggtgtt acgggattca tcgttagaga tgagatccag ataacgctga
     3721 cgatagcgcg cttcctgatc ctgcaagccg tggaatttat ccggcagcgg acgcagtgct
     3781 ttggtcagca gacgcagctc ggtacagtgg atagacagtt cgccggtttt ggttttgaac
     3841 agtttacctt tcgcgccgag gatgtcgccg aggtcccatt ttttgaactg ctcgttgtaa
     3901 acgccttccg gcagatcgtc acgggcaacg tacagctgaa tgcggccacc cacatcctgc
     3961 agggtaacga aagacgcttt acccataata cggcgggtca tcatgcggcc agcaacggcg
     4021 acttcgatgt tcagcgcttc cagttcttcg ttctctttac cgtcgaattc tgcgtgcaat
     4081 tggtcagagg tatgatcgcg acggaaatcg ttcgggaagg caatcccctg ctcgcgcagg
     4141 ttcgccagct tctcacgacg cgttttcagt tcattgttaa gatcgactac cgcgtcagcg
     4201 ccctgtgcgt gttgttcaga catgttggtt cctcataacc ctgctttcaa acttgcttcg
     4261 ataaattgat ccaggctgcc gtccagcacg gcctgcgtgt tgcgagtttc taccccggta
     4321 cgcagatctt taatgcggga gtcatcaagg acataagaac gaatctggct gccccagccg
     4381 atgtcagatt tgttatcttc catcgcctgt ttctcggcat ttttcttctg catctccagt
     4441 tcataaagct tcgctttcat ctgcttcatg gcctgatctt tgttcttgtg ctgggaacgg
     4501 tcgttctggc actgggtcac gatcccggtc gggatgtggg taatacgcac cgcagattcg
     4561 gtacggttaa cgtgctgacc gcccgcgccg gacgcgcgat aaacgtcaat gcgcagatcc
     4621 gccgggttga tttcgatatc aatatcatca tcaacttccg gataaacaaa cgcagagctg
     4681 aacgacgtgt ggcgacgacc gccggagtca aacgggctct tacgcaccag gcggtgaacg
     4741 ccagtttctg tacgcagcca gccgtaagcg taatcgccgg agattttgat cgtcacggat
     4801 ttaatacccg ccacttcacc ttccgactct tcgatgattt cagttttgaa accacgcgat
     4861 tctgcccagc gcagatacat acgctcaagc atgctcgccc agtcctgtgc ttccgtaccg
     4921 ccagaccccg cctgaatatc gaggtagcag tcggcgctgt catattcgcc agagaacata
     4981 cggcggaact caagctgcgc cagtttttct tccagggcgt cgagttcagc aacggcttcg
     5041 ttaaaggttt cttcgtcgtc agcttctaca gccagttcca gcagaccaga aacatcttcc
     5101 agcccctgtt tcatttggtc gagggtgtcg acaacggctt cgagggagga acgctcttta
     5161 cccagcgcct gtgcgcgttc gggttcattc cagacatccg gctgttccag ctcggcgttt
     5221 acttcttcca gacgctcttt cttggcgtcg tagtcaaaga taccccctaa gaacgtcgga
     5281 gcgttccgtg aggtcctgaa tgcgattatt taccggatta atttcaaaca tggtctgatt
     5341 tcttttattg agctagtcaa aatgcggtga taagagcggg attgtaccca atccacgctc
     5401 ttttttatag agaagatgac gctaaattgg ccagatattg tcgatgataa tttgcaggct
     5461 gcggttgccg cgaaactcgt tgatatcgag cttgtaagcc agttgcactt cgcgcacgcc
     5521 gttatccggc cagagggcgg tatcgacatt aaaagcaata ccatccagca gcggaccgcc
     5581 gccgaccggt tcgaccatga ctttcaaatg acgttcgccc accagccgct gttgcagcag
     5641 ccggaaatga ccgtcaaaca gcggctccgg gaacatctgc ccccacgggc cagcatcgcg
     5701 cagcagctgc gccacttcca tggtcatttc ggccgggctt aacggaccgt ctgacaccac
     5761 ttcgccttgc aatagcgaag ggtccagcca ctcagtaacc agttcgccaa accgttgttg
     5821 aaagagttcg aatttatccg cttccagcga caaacccgcc gccatcgcat gaccgccaaa
     5881 cttcagcatc atgccagggt agagtgtgtc taatcgctcc agcgcatcac gcatatgcag
     5941 cccctgaatg gagcgaccag aacctttcag cgtaccatca cccgcgggcg caaaggcgat
     6001 aaccggacgg tgaaaacgct ctttaatacg ggaagccaga ataccgacaa ccccctgatg
     6061 ccattcgggg tgatacattg ccagcccgcc gggtagcgtg tcacggctgc gctccagttt
     6121 ctcgcacagg gtcagggctt caacctgcat tccttgttcg atctcttttc gcgtctggtt
     6181 tagcgcatcg agttcatttg ccagcacgcg cgcttcgccg atgttgtcgc acagcaagag
     6241 cgccacaccg acggacatat cgtccagtcg tccggcagca ttgagacgtg gccccagcgc
     6301 aaaacctaaa tcgctggcgg cgagtttttg tgcatcacgg tttgccactt caagcagcgc
     6361 tttaatcccc ggacggcact ttccggcacg gatgcgactc atcccctgcc aggtcagaat
     6421 gcgattatta gcgtccagcg gtacgacgtc cgccactgtc cccagcgcta ccagatccag
     6481 cagttctgcc aggttaggaa ttgcgatgcc gcgctcatca aaccagccct gatcgcgcaa
     6541 aaaggtgcgc agcgccagca tcagataaaa cgccacaccc acgcctgcca gtgatttcga
     6601 cgggaaatta cagtcgcgca agttagggtt aatgatcgct tccgccgcgg gtaatgtttc
     6661 gcccggcaaa tggtgatcgg taacaataac cggaatgccc aacgagcgag cgtgttcaac
     6721 ccccgcatgg gaggaaatac cgttatccac cgtgacaatt aactgcgcac cacgggcatg
     6781 ggcctgatcg accacttccg ggcttaagcc gtaaccgtct tcgaaacggt ttggaaccag
     6841 gtagtcgata ttgctgcaac caagcgagcg catcgccagc acgcttagag ccgtgctggt
     6901 ggcaccgtcg gcgtcgaaat caccgaccac aataatccgc gttccttcgc gaaaggcgtt
     6961 gtaaaggatc tcaacggcct tttcgacgcc gctcagttgc tgccagggca gcatcccttt
     7021 aacactgcgt tccagttctt gcgcactgcg cacgccccgg ctggcgtata aacggcgcag
     7081 caagggaggc aattcagcgg gcaagtctgc cgtttcatcg actgcacggc gacgaagttg
     7141 tatctgttgt ttcacgcgaa ttatttaccg ctggtcattt tttggtgttc gtcgaggaat
     7201 tctttcatct ctttcggcgg ctggtaaccc ggaacaagtg tgccattgct cagcacaact
     7261 gccggagtac cgctaacgcc aagctggacg ccaagtgcgt aatggtcggc aatatccacg
     7321 tcgcagctgg ctggtgcgac gcttttacct gccatcacat catcaaacgc tttgttttta
     7381 tctttcgcac accagatagc tttcatttct ttctctgcat cgctgtccag cccctggcgc
     7441 gggaaagcaa gataacgcac ggtgatcccc agcgcgttat agtctgccat ttgctcatgc
     7501 agtttgtggc agtaaccaca ggtaatatca gtaaacacgg tgatgacgtg tttttcctgc
     7561 agcgctttat aaacgatcat ctctttttca agcgcattca actgctttaa cagcatctta
     7621 ttggtgacat tgaccggagc cgtgccgcta acgtcataca ttggcccctg aatgatatgt
     7681 ttaccatcat cggtgatgta caacacgccg ctgttagtca gaactgtctt catgccagct
     7741 acgggcgcgg gctgaatatc gctgcttttg atgcccattt tggctaacgt ttgttgaatt
     7801 gccgcgtcat cagcctgagc aaaacctgaa aacgccgcta acaaagtaaa caacataaaa
     7861 cctttcttca taaatcttcc cgttcttttc agacatcacg cccgcgggtg atgctgttga
     7921 tgaagttgcc gcagacgctc ggtagcgaca tgcgtataaa tttgcgtggt ggaaagatcg
     7981 ctgtggccca gtagcatctg caccacgcgt aaatccgcac catgatttaa taaatgagtg
     8041 gcaaaagcgt gacgcaacac atgcggtgac agcttttcgc tgtcgatacc cgccagcacg
     8101 gcataatgtt taatgcggtg ccagaaggtc tgtcgcgtca tctgctgcgc acgctggctg
     8161 ggaaacaaca cgtcaattga cacaccattc agcagccacg gacgcccatg ttccagatag
     8221 gtttccagcc agtaaaccgc ctcttcacct aacggtacca ggcgctcttt gttgccttta
     8281 ccaatgaccc gcactacgcc ctgacgcagg ctgatatcac tcattgtcag cccgaccagt
     8341 tcagagacac gcagcccggt agcatacaac acttcaagca tggctttatc gcgtagctcc
     8401 agtggctgat caattaatgg cgcctgtaat aaacgttcga cctgcgcttc gcttaaatct
     8461 tttggcaaac gctggggcaa tttcggtgaa gcgagatgcg cactgggatc gtcttcacga
     8521 aacttttcgc gataaagata ctggaacaat cggcgcactg cactcagcaa acgcgctgag
     8581 ctggtggctt tatacccgcc ctccagccgt tctgccagta atgcctgcaa atcgtcactt
     8641 tgcgccgtcg ccagcgtcaa cccgcggtga tgcaaccact ccaccatcat tgacagatcg
     8701 cgacggtaag cgttcaacgt attttcagcc agattttttt ccagccacag agcatcaaga
     8761 aactgctcga tgcgtgccag atcctgtttc acttgcgccc cttatggtca ctcatttgat
     8821 ccattatgcc ttattgtgcc gtgactaaag cgattctgat acactagctg caaaagccac
     8881 agcagaatcg agaagcttac gttatgaata tgggtctttt ttacggttcc agcacctgtt
     8941 acaccgaaat ggcggcagaa aaaatccgcg atattatcgg cccagaactg gtgaccttac
     9001 ataacctcaa ggacgactcc ccgaaattaa tggagcagta cgatgtgctc attctgggta
     9061 tcccgacctg ggattttggt gaaatccagg aagactggga agccgtctgg gatcagctcg
     9121 acgacctgaa ccttgaaggt aaaattgttg cgctgtatgg gcttggcgat caactgggat
     9181 acggcgagtg gttcctcgat gcgctcggta tgctgcatga caaactctcg accaaaggcg
     9241 tgaagttcgt cggctactgg ccaacggaag gatatgaatt taccagcccg aaaccggtga
     9301 ttgctgacgg gcaactgttc gtgggtctgg cgctggatga aactaaccag tatgacctta
     9361 gcgacgagcg tattcagagc tggtgcgagc aaatcctcaa cgaaatggca gagcattacg
     9421 cctgatctca ctgacggctt agcgcatatg ctttgccgtc atctttatct ttgcgtctct
     9481 tgttgcaaca aaatccgccg taaatcccgc cattcggctt cgtccatgct gtcggctgcc
     9541 agccataaat gttgccgttt accgccatca gaacgtaaac gcagcatcat gccgctctta
     9601 atcatccacg gtgctttgac gatgctccac tcctgccctt gccaacgcaa acgcccgtcc
     9661 atcaacaagc gaatttcccc ctggcgagca ttaatacgcc gctggctgcg aacgcaatca
     9721 aacaccacca gcgaaagtaa caccatccat aacggggtgt aactgagtgg ccagggcatg
     9781 agtaaaataa cagcggcaac cagcccatga atcagcaagg aaagccactg tgcgcgccag
     9841 gagacgcgca aatcagattg ccacaggacc acgttcccgg ttccgtgtct ggatgagtcg
     9901 gaccatcatt tccagttctg catcggccgg tttaccgtga ttcatcagcc agttaaacag
     9961 gtccggatcg tcacattcca gcagacgaat aaagatgcgt ttttcgtcat cgcttaagct
    10021 gtcgtactca tgttcgaaaa acggcatgat tgaaatatcg agttcgcgca taccacggcg
    10081 gcatgcccaa tgaatgcggg ctttgttgtt aatgtccatc ttcttcctgt ctcacgaaaa
    10141 tccagtaccc cgctattgta acgtgttttt cgccttcttt tacgggaata tcagtaaaca
    10201 ccatcgcgat cacgaaatta attcacaaca attcaatggc ttcatttttt tggaagtcgc
    10261 ctcgcagaag gcacagatcg cgtagtgaaa gcacttgcat tgcctcatag ctcttttacc
    10321 attagtcatt aatacgccgt taagcaactc aggactctat tatggctttt acaccttttc
    10381 ctccccgtca gcctacggct tctgcccgtt taccgctgac gctgatgacg cttgatgact
    10441 gggcgcttgc caccattact ggcgcggaca gcgaaaaata tatgcagggt caggtgacag
    10501 cagatgtcag ccagatggca gaagatcagc acctgctcgc cgcccattgc gacgccaaag
    10561 gcaaaatgtg gagcaattta cgtctgttcc gcgacggcga tggctttgcg tggattgaac
    10621 ggcgcagcgt gcgtgaaccg cagctgactg aactgaaaaa atatgcggtg ttctctaaag
    10681 tgaccatcgc gccagacgac gagcatgtgc tgcttggtgt tgccggtttt caggcgcgcg
    10741 ccgcgctggc aaatatcttt agtgaactgc cttcgaaaga aaaacaggta gttaaagaag
    10801 gcgcgaccac tttgctatgg tttgaatacc cggcagaacg tttcctgatc gtaaccgatg
    10861 aagctactgc taatatgctg accgataaac tgcgcggtga agcggaactg aacaatagcc
    10921 aacagtggct ggcattaaac attgaagcgg gtttcccggt gattgatgcc gccaacagcg
    10981 ggcagtttat cccacaggcg accaatctcc aggcgctggg cggtatcagc tttaagaaag
    11041 gctgttatac cggacaagag atggtggcgc gagcaaaatt ccgtggtgcc aataaacgtg
    11101 cgctctggtt gctggcaggt agcgccagcc gactgccgga agctggtgaa gacttagagc
    11161 tgaaaatggg cgagaactgg cgtcgtaccg gtacggtgct ggctgcggta aaactggaag
    11221 atggtcaggt cgtggtacag gtcgtcatga ataacgatat ggaaccggat agcatcttcc
    11281 gcgtacgcga cgatgcgaat acattgcata ttgagccgct gccgtattcg ctcgaagagt
    11341 aaatctcttt atcgcatcag gcatttatcg cctgatgcga cgctgacgcg tcttatcatg
    11401 tctgggattt gatgccttta tcacaacagg gacgaggccc gtagatcgga taaggcgttc
    11461 acgccgaatc cggcagttgt gctccgatgc ctgatgcgac gctgacgcgt cttttcaggc
    11521 ctacaaaggc atacccatta cgcctgccca atatacaaat agatcgccag aaagtggcac
    11581 acactgccgc cgagcacgaa gccgtgccag atggcatgat tgtagggaat gcgtttgcag
    11641 acatagaaaa tcactccgag cgaataaacc acgccgccta ccgccaataa ggtaacgctg
    11701 cccgccgcga gcttaactgc catttcataa attaccacca gcgacagcca gcccatcgcc
    11761 agataggtca ccagagataa aattttgaat cggtgcgcga tggtcagttt aaacagaata
    11821 cccagcaatg ccaggctcca gataacaatc atcaacccgc gcgctaacgg agaatccagc
    11881 cccaccagca aaaacggcgt gtaggttccg gcaatcaaca ggtaaatagc gcaatggtca
    11941 aatttcttca gccacatttt tgcccgttga tgaggaatgg cgtgatagag cgtcgaagcg
    12001 aggaacagca ggatcatact gccgccatag aggctgtaac tggttatcgc tgtggcgctg
    12061 gcattgagat ccaccgcctg aaccagtagc aacaccagcc caacgatacc aaacaccaac
    12121 ccaatgccgt gactgacgct gttggcaatt tcctctgcca gcgaatatcc ctgcttaatg
    12181 aggggcttct gaaccataac ttactccgga gaaacgtaca cgcacatgta tacctctcca
    12241 gcgtaactga gaatagttcc agtgaacacc tgttagctaa aataaattct gatttgagac
    12301 taatctccta aaaatcatga aattaaatgc gaaatttcaa ctaacaggcg tgagttcaat
    12361 ttaaagacat ttaaattcaa tcacataaaa ctgtgtctga ccgggataga tgtcggcaat
    12421 gacctttatc agttcagtca gggtcatatt ttcctgctct gcatgttttt ccgtcagcgt
    12481 atccagcgtt acggttgagg ttgcggtgac ttcaatcgtg caaaaataac cgtcatcttc
    12541 aaaacgcccg acacgaagca catcacccgt tttgaagtgc gattcagact cgtcgcggat
    12601 ggtgatggtt ttacgcccag ccagaatgtc atcctggaaa cgttgaaaaa aagtgatgtc
    12661 gtttggctgc atggtactat ttcctgtaag aattgactca tctggagcct atgatagtga
    12721 aaaaactcac cttacagaaa gatttcttat ggggcggcgc agttgccgct catcaggtcg
    12781 aaggcggctg gaacaaaggc ggcaaagggc cgagcatttg tgacgttctg accggtggcg
    12841 cacacggcgt gccgcgcgaa atcaccaaag aagtcttgcc aggcaaatac tatccaaacc
    12901 atgaagccgt tgatttttat ggtcactaca aggaagacat caagctattt gccgaaatgg
    12961 gcttcaaatg ttttcggact tccatagcct ggacgcgcat ttttccaaaa ggcgatgaag
    13021 ctcagccaaa cgaagaaggg ctgaagttct acgatgatat gttcgatgaa ctgctgaaat
    13081 acaacatcga accggtgatc accctctccc actttgaaat gccgctgcat ctggtgcagc
    13141 aatacggtag ctggaccaac cgtaaagtgg ttgatttctt tgtccgtttc gcggaagtgg
    13201 tgtttgaacg ctataagcat aaagtcaaat actggatgac cttcaacgaa attaacaacc
    13261 agcgtaactg gcgtgcaccg ctgttcggtt actgctgctc cggcgtggtt tataccgagc
    13321 atgagaaccc ggaagagacg atgtatcagg tgctgcatca ccagtttgtc gccagcgccc
    13381 tggcggtgaa agctgcgcgt cgcattaacc cggagatgaa agtcggctgt atgctggcga
    13441 tggtgccgct ctatccctac tgctgtaacc cggacgatgt gatgttcgct caggagtcga
    13501 tgcgcgaacg ctacgtcttt accgatgtgc agctacgcgg ctattacccg tcctatgtgt
    13561 tgaacgagtg ggagcgtcgc ggatttaaca tcaaaatgga agacggcgat ctcgatgtac
    13621 tgcgcgaagg cacctgcgat tatcttggtt tcagctatta catgaccaac gcagtgaagg
    13681 ccgaaggcgg caccggcgat gcgatctctg gtttcgaagg cagcgtaccg aacccgtatg
    13741 ttaaagcgtc tgactggggc tggcagattg atccagtagg tctgcgctat gccctttgcg
    13801 aactgtatga gcgttatcag aggccgctgt ttattgtcga aaacggtttt ggcgcttacg
    13861 acaaagtgga agaagatggc agcatcaacg acgactaccg cattgactac ctgcgcgccc
    13921 atatcgaaga gatgaaaaaa gcggtgactt acgatggcgt ggatctgatg ggctacacac
    13981 cgtggggctg catcgactgc gtatcgttca ccaccgggca gtacagcaaa cgctacggct
    14041 ttatctatgt gaataaacat gacgacggta ctggcgatat gtcgcgttca cgtaagaaga
    14101 gctttaactg gtacaaagag gtgattacca gcaacggcga gaagctttaa gtcgatgaag
    14161 taccggatgc aatacttgtt gcatccggtc agacaacttc cctgttttat ttcccgcccg
    14221 ccaaatcgat aaaacttccc gtgacgtaag aggctttatc acttagtagc cagacaatgg
    14281 cctgcgcgac ctcttctgcc tgtccaccac gctgcatggg gatgttcgac ttaacgcgat
    14341 cgacgcgtcc aggctcgccg ccgctggcgt gcatttcggt aaaaataaac cctggccgca
    14401 cgcagttaac gcggaccccc tgcgcggcga cttccagcga tagtccggtg gttaacgtat
    14461 caatcgcccc tttcgatgcc gcgtaatcaa catattcccc tggcgaaccc aaccgtgagg
    14521 ccaccgaaaa gacattgacg atagcgccgc cactgccacc atttttaagc gccatgcgtt
    14581 ttaccgcctc gcggcagcag agaaaatatc ccgtcatgtt ggtggaaagt actcggttga
    14641 ttcgctctgc ggtaaggttt tcaacggtgc actgggtaaa caagatcccg gcgttattga
    14701 ccagcgctgc tagcggttca tcgtgctgat cgattgctgt aaacattgcg atgacctgat
    14761 tttcgtcgct gatatccgcc tggagcacga atgccttgcc accggcttgc gtgattaagt
    14821 tcaccacttc ctgcgccgca tggaggtttt gttgataatt aaccgccacc gtatactctt
    14881 ctttcgccaa cagtaatgca gttgcccgcc cgatgccgcg actgccatcg gtcacaagtg
    14941 ctatagccat ttgtttttcc gagaaaaatt aattcgttga cgtatctttg agattgctaa
    15001 caaactggct caatcttcaa tgttggcacg cttttcccct cgccctttca gggagagggc
    15061 cggggtgagg gtaaatattc gcgccagtgc cggcctgttc ccctcaccct aaccctctcc
    15121 ccaaaggggc gaggggactg tctgagcact tttgtacttt gtcatctgac taaaaaggcg
    15181 ccgaagcgcc tttagaaaat agtcgaatca gtgaattact ggtattcgct aatcggtacg
    15241 caggagcaga acaggttacg gtcgccgtaa acatcatcca gacgtttcac cgtcggccag
    15301 tatttgtctg ccacgcctgc cgggaatacc gcaacttcac ggctgtacgg atgcgcccac
    15361 tcggcgacca gttcgctctg aatgtgcggc gcgttcacca gcgggttatc ttccagcggc
    15421 cagacagcgg ctttcacctg gtcaatttct gcgcggatag ccagcatcgc gtcgataaag
    15481 cgatccagtt ccactttgct ttcagattca gtcggttcaa ccatcagcgt acccgccacc
    15541 gggaacgaca tcgtcggcgc gtggaaaccg tagtcgatca ggcgcttggc aatatccagc
    15601 tcgctgatgc cggtttcttc tttcagcggg cgaatatcga gaatacattc gtgcgccacg
    15661 cgaccgtcgc gaccggtata cagcaccggg aaggcatcct gcaggcggct ggcaatatag
    15721 ttggcgttga ggattgccac ctggcttgct tttttcagcc cttctgcgcc catcatgcgg
    15781 atgtacatcc agctgattgg caggatagag gcgctaccga acggtgccgc agaaactgcg
    15841 ccctgacggg ttaacatgcc ttcgatttgc accacgctat gacccggtac aaacggtgcc
    15901 agatgcgctt tcacaccgat cggtcccata cccggaccac caccgccgtg cggaatgcag
    15961 aaagttttat gcaggttgag gtgcgaaacg tccgcgccaa taaagcccgg cgaggtgatg
    16021 ccaacctggg cgttcatgtt cgcgccatca aggtaaacct gaccgccgaa ctgatgcacg
    16081 acttcacaca cttcacggat cgtttcttca tacacgccgt gggtagaagg ataagtcacc
    16141 atgatacagg agaggttatc gcccgcctgt tccgctttcg cgcgcagatc agtcagatcg
    16201 atgttgccgt ttttatcaca cgccacaacc accacctgca ttcctgccat atgtgcagaa
    16261 gcggggttag ttccgtgcgc agaagccggg atcaggcaga tatcgcgatg cccttcgttg
    16321 cggctttcat gataatgacg aatcgccagc aggcccgcgt attcgccctg tgcgccggag
    16381 ttcggctgca tacaaacggc gtcgtaaccg gtcagtttca ccagccagtc agccagctgc
    16441 gcaatcatct gctgataacc ttcagcctgt tccggcgggc aaaacgggtg cagttcggca
    16501 aattccggcc aggtgattgg gatcatctcg gcggcggcgt tcagtttcat ggtgcaggaa
    16561 cccagcggga tcatcgcctg attcagcgcc agatctttac gctccagcga gtgcatatag
    16621 cgcatcattt cggtttcgct gtggtagcga ttaaacaccg gatgggtgag gatttcgtcg
    16681 tcgcgcagca tcgcaggctg gatagagcgg ctgtcgtgag ccacgtcttt gtccagcgtg
    16741 tcgatctcca gcccgtggtt atcgcccagc agcacgctga aaagctgcat tacgttttca
    16801 cgcgtggtgg tttcatcaag ggtgatcccc acggcgttca ggatatcgct acgcaggttg
    16861 atttcagccg cttcggcacg cgtcagtacg cccgctttgt cggccacttc cacacacaag
    16921 gtgtcgaaat agtgcgcatg gcgcagtttc agaccttttt gttgcaggcc cgccgccaga
    16981 atatcggtca gacggtgaat gcggttagcg atacgtttca ggccaaccgg gccgtgataa
    17041 acggcataca ggctggcgat gtttgccagc agtacctggg aagtacaaat gttggagttc
    17101 gctttctcac gacgaatatg ttgctcgcga gtctgcatcg ccatgcgcag cgcggtattg
    17161 ccagctgcat ctttcgatac accgataata cggcccggca ttgagcgttt gtattcatct
    17221 ttcgccgcaa agaatgccgc gtgtgggcca ccgtagccca tcggcacgcc gaagcgttgc
    17281 gccgaaccaa aaacaatatc cgcgccctgt ttacccggcg cagttaacag caccagcgcc
    17341 ataatatcgg cggcaacgct gaccacaatt ttgcgtgatt tcagttcgct aataagcgca
    17401 gtgtagtcgt gaatttcacc ggtagtgcct acctgctgta acagcacgcc gaagacgtcc
    17461 tgatggtcga gcactttttg cgcgtcatcg acaatcactt caaaaccaaa ggtttcggca
    17521 cgagtacgga ccacatccag cgtttgcgga tgcacatcgg aagccacgaa gaagcggttg
    17581 gcatttttca gtttgctgac gcgtttcgcc atcgccattg cttcggcggc agcggtggcc
    17641 tcgtccagaa gagaagcaga ggccatatcc agtccagtca aatccagcgt tacctgctgg
    17701 aagttgagca gtgcttcaag gcggccctgg gagacttcag gttgatacgg agtgtacgcg
    17761 gtataccagc ccggattttc cagcatgtta cgcaggataa ccggcggtag ctgcacggcg
    17821 gtgtaaccca tgccgatgta agacgtgaag cgtttattgc gactggcaat agccttgagt
    17881 tctgccagtg cggcgtattc ggtcgccggt gcgccaacct gcggcggggt cgcaagctga
    17941 atatctttcg gtacaatctg gccggtcagc gcgtttaacg attgtgcgcc aacggcattc
    18001 agcatttctt gctgttgcgc ggcgtccggt ccgatatggc gttcaataaa agcgccgctg
    18061 ttttcaagct ggcttaacgt ctgtgtcatg agcgatggtt cctgaaacgt gcagtgaatt
    18121 gtgaacctct ctccttacga agagagtgag ggtgaggcat aaattttcct caccctgatc
    18181 ctctcccgca gaagaggaat aaagccgtta ctcgtcttct aacaatgctt cgtatgcggt
    18241 cgcatccagc agtgattcca gttcgctttc atcgctggct ttgattttaa agatccagcc
    18301 acctgcatac ggttcgctgt tcaccagttc cggggaatcg ctcagtgcgt cgtttaccgc
    18361 cacgatttca ccgcttactg gcgcataaat gtctgacgcc gcttttactg attcggcaac
    18421 cgcgcagtca tcgcccgcgc taaccgttgc gcccacttcc ggcaggtcaa caaacaccat
    18481 atcgcctaac agctcctgag catgttcggt aataccaacg gtgtaagtgc cgtcggcttc
    18541 tttacgcagc cattcgtgtt ctttgctgta tttcagttct gctggtacgt tgctcatcaa
    18601 tcaatctcca aaaaagtaaa tcacgcgacg gctttgccgt tacgcacaaa aacaggtttt
    18661 gtcactttaa ccgacatttc acggttgcga atttgcacaa tcgccgtttc gccaatacct
    18721 tccagcacgc gcgccagcgc aatgctgtaa cccagcgtcg gggagaaagt accgctggtg
    18781 ataatgcctt catgctggtt gccctgcgca tcggtaaagc gtaccggcag ttcattacgc
    18841 agcacgcctt tttcggtcat caccagacca accagttttt ctgtaccatg ctcacgctgc
    18901 acttccaggg cttcacgacc gataaagtca cgatctgccg gttcccaggc gatagtccag
    18961 cccatgttgg cggctaaagg agaaatagtt tcgtccatct cctgaccata aagattcatg
    19021 cccgcttcca gacgcagcgt gtcacgcgcg cccaagccac atggcttaac acccgcttcc
    19081 accagcgcac gccagaaatc ggccgctttt tcattgggca gcgcaatttc atagcccgct
    19141 tcaccggtat aaccagtggt ggcaataaac agatcgcccg cctgcacgcc aaagaacggt
    19201 ttcatccctt ccaccgcctg acgctgggcg tcattaaaca gtgtggcagc ttttgcctgc
    19261 gcattcggcc cttgcacggc aatcatggaa aggtcatcac gaacggtaat ttcgatgccg
    19321 aaaggttcag cgtgttgggt aatccaggag aggtcttttt cgcgggtggc ggagttaaca
    19381 acgaggcgga agaaatcttc agtaaagtag tagacgatga ggtcatctat cacaccgcca
    19441 gaggcattca acatccccga gtaaagggct ttgccgcttt tggtgagctt cgccacatcg
    19501 ttcgccagca gataacgcag aaactcccgg gtgcggctgc cgcggagatc gacgatggtc
    19561 atatgtgaca catcaaacat tccggcatcg gtacgtaccg catgatgttc gtcgatttgc
    19621 gaaccgtaat gcagcggcat catccagccg tggaaatcca ccatgcgagc gccgcaaagc
    19681 gtgtgttgtt cgtacaaagg agtctgttgt gccatcttgt cctcattgaa taagcggggc
    19741 tgacaacttt tcatggtgaa attatcacca cgaaacccag catcggagcc actcccggtc
    19801 cccaacgcaa tcgttctctt ttgcctgaac ttaccaccga aacagactgt taaccataag
    19861 gtaaaattga tcatcacatt agcttatggt taaaaaacgc caaaatcgcg acagaataaa
    19921 aaaccaaaaa acacaccagt ttctatataa agatgatgcc attagaaagc taatttgaat
    19981 aaggcaatat taagagctaa aaaaatgtca aaaaacacta aatcaaaaaa taatgacatt
    20041 agaaaatata atgcgaaaac ggaggtgaaa ttagtttatt tcaaatgagg aaaatctccc
    20101 ggcgaaaaaa ccgggagatg aaagtgtgat gggtatcaaa taaacagcag aagagaaatt
    20161 tttaacgcag ccattcaggc aaatcgttta atcccattgc ctggcggata agttgtggtt
    20221 taacgccagg aagcgtgtcg gccagtttca aaccaatatc acgcagcagt tttttcgccg
    20281 gattggtacc ggaaaacaga tcgcggaatc cctgcatacc agccagcatc agcgccgcac
    20341 tgtgcttgcg gctacgctca taccggcgca ggtaaatgta ctgcccgatg tctttcccct
    20401 gacgatgcaa ccgtttcagt tcagcaatca gctctgcagc atccataaag ccaagattta
    20461 ctccctgccc cgccagcggg tgaatggtat gcgcggcgtc gcccaccagc accagacggt
    20521 gcgcggcaaa ctggcgcgca taacgccccg tcagtgggaa cacctgacgc gcgctctcaa
    20581 ccttgcataa gcccaggcga ttatcaaaag cgatatttaa cgcgcgatta aattcatctt
    20641 cacttgcctg ctgcatccgc tgcgcttcct ctggcgacag tgaccagaca attgagcaaa
    20701 gatgcggatc gctaagcggt aaaaaggcca gaatgccttc gccatggaaa acctgccgcg
    20761 ccaccgcatc atgcggttct tccgtgcgaa tggtcgctac cagcgcgtga tgctgataat
    20821 cccagaaagt cagcggaata tctgctttgt tgcgcaacca ggaattagca ccgtccgcgc
    20881 caatcaccag acgcgccgtt aacatgctgc catctttcag cgtcaggaaa gtttcatttt
    20941 ctccccaggc gacctgctgt aattctgcgg gggccaacag agtgatatct gacgactgct
    21001 gcgctttgtt ccacagcgcg tagtgaatca ctgagttttc aacgatatgc ccaagatggc
    21061 tatagcccat gctttgatcg tcaaacgaaa tgtgaccaaa actgtctttg tcccacactt
    21121 ccataccgtg ataacaactg gccctacgag agagaatgtc ctgccagacg ccaagacggg
    21181 tgagtaattt ttcgctggcg gcattgatag ccgaaacgcg cagttgtggt ggtgcatccg
    21241 ccgccagagg ttccggtacg cgctgctcca gtacggcaac gcgtaagccg ctcccctgta
    21301 agccgcaggc aaccgccagc cccaccatgc cgccaccaac aatggctaca tcaacacttt
    21361 gcattgttta ttccttaaaa ccgccttcaa cgcgccaccc aaccgagggt gcgctgcgcc
    21421 agcacatcgc gtgccggggt gaataattcc atcgtcatca gtccgatgtt gcgcccgaca
    21481 accagcggtg cccaacggtt ggcaaaaaga tgtaccaggc tgtccgtgac gccgatggtt
    21541 gcttcgcgat cgctctgtcg acgctgctga tagcggcaca atatgccgta atcaccgatg
    21601 tcttctccgc gctcatgcgc ctgagtcagg gtttccgcaa ggctcatgac atctcgcata
    21661 ccgaggttaa acccttgccc ggcaatcggg tgcagagttt gcgccgcatt gcccaccagc
    21721 acggtacgat gggtaatagc tctggcggcg cgggttaacg ccagcggata agcactgcgt
    21781 ttaccggcgt gggtaatttt tccaagtcgc cagccaaagg ccgactggag ttcacggcaa
    21841 aacttctcgt cgctccacga cagcacctct tcgcgccgtt ccagtggatg acaccagacc
    21901 agcgaacagc gtccgtcaga catcggcaac atcgccagcg ggccatgttg cgtgaagcgt
    21961 tcaaaagcgc ggccttcgtg cgcaacggaa gtcgcaacgt tggcaatcac ggccagttgt
    22021 tcgtaaggct cctgctgcca gtcaacgccg cacgcggtgg ctaacgctga atgggtgcca
    22081 tcagctgcta ccagcacgcg gcccgtcagc gtctcgccac tctccagcgt cacttcaacg
    22141 tgactctgag tacgggcaac gttagccacg cgatcagggc aatgcagcgt tacgccaggt
    22201 gctttacgca gcaatgcaaa cagccgttgc ccgacattgt gcaattcgac aacctgtccc
    22261 agcgccgcca gttggtaatc ttctgcggcg agggtgacaa atccagcgtg accacgatcg
    22321 ctgacatgca cggtagtgat ggcggttgcg taatccgcca gagattgcca gacgccgatg
    22381 cgcgccagtt gctgacaggt ccccgcagcc agcgcaatag ctcgtccatc aaagcccgga
    22441 tgagcatgtg actctggcgc ggtcgcttca atcaaatgta ccggcagcgc cccgtgactt
    22501 aaccgggaaa tagccagcgc cagcgtcgcg cccgccatgc cgccaccgac gatgattacg
    22561 ctcattgctt tctcgcagct gccatcaacg cttcgatttc ttccggcttt ttcaccacgc
    22621 tggcggtgag gttttcgttg ccggtttcgg taatcacaat gtcgtcttca atacgaatgc
    22681 cgataccgcg atattgttct ggcacatccg catccggcgc gatatacaga ccaggctcta
    22741 cggtcagtac catccccggt tccagaatgc gcgagcgatc ctgaccataa acgccaacgt
    22801 catggacatc cagtcctaac cagtggctaa ggccatgcat aaagaaagga cgatgggcgt
    22861 tctgagcgat cagttcatca acatcacctt tcaggatgcc gagttttacc aggccgctaa
    22921 ccatgatgcg caccacttca ccagtgactt ccagaatgga agttcccgga cgatacaggc
    22981 gcaggctggt ttcgagagac tccagcacaa tgtcgtagat ttcacgctgg gcctgggtga
    23041 atttgccgtt gaccgggaag gtgcgggtaa tatcgcccgc gtaacctttg tattcacaac
    23101 ccgcgtcaat caacaccagg tcaccgtcgc gcagttcact ctcgttttcg gtgtagtgca
    23161 gaatgcagcc gttttcaccg ctgccgacaa tggtgttata ggacggatag cgcgcaccgt
    23221 ggcggttaaa ttcgtggtga atttcgcctt ccagatggta ctcgaacatt cccggacggc
    23281 atttttccat cgcccgtgta tgtgccatgg cggtgatttc tcccgcgcgg cggagtacgg
    23341 caatctcttc tggcgatttg aacaggcgca tttcatgaac aacaggacgc cagtcgatca
    23401 tcgttgccgg tgcggtgaga ttttgccgcg aacctttacg cagtttttcc agcgcactgt
    23461 tcatgatttc atcagcatat gcatattcgc cctgggcatg gtaaaccaca tccaggccgt
    23521 taagtagttg ataaagttgc tgattgattt cgctgaatgc cagtgcgcgg tcaacgccca
    23581 gtttctctgg cgcggcatcc tggcctaagc gacggccaaa ccagatctcc gccgtcaggt
    23641 cgcgaacgcg gttaaacaga acgctgtggt tatgagtgtc atcgctttta atcagcacca
    23701 gcaccgcttc caattcgtta aagccggtga agtaccagaa gtcactgttc tgacgatagg
    23761 ggtattcgct gtcggcgcta cgtgttactt ctggtgcagc aaaaatcagc gcggcgctgc
    23821 cgggttgcat ttgctccacc agggcctgac ggcgacgctg aaactcttgc cgggatatct
    23881 cactcataac actctcctta cgttttttgt ttttagtgta gagtcggttt ttgtacttct
    23941 ggcgcggtcg gttgcggatg agtaaaggtg tcgtggcata acagcgcggc aacacgaacg
    24001 tattcgatga tctcttcaag cgacatttca agctcttcct gatcttcgtc ttcgtcgtaa
    24061 cccagttgcg caatgttacg cagatcgtcg atagcttcac cggtttcgcc ggtcacttta
    24121 tccagcttcg gttgcgtaac gccaagacca agcaggaagt gattgaccca acctgccaat
    24181 gcatcagccc gatcgaaaac gctgacatca tcgccatcag gcagataaag ctgaaaaagg
    24241 aagccgtcat cctgcagggc atcgctggtg gcagagtgca ttttacgcag tgcctgtgcc
    24301 agctcatgac cgaaagccat gccttcgttc gtcaggtcgt gaagtagcgg cagccatgag
    24361 ctgtcatcgt taccgccaca tatcatccca ctgattaaac catgcatctc ggcaggggtc
    24421 agccccgtcc cttgttggtt cagatactgg ttcatttcgt tgtaaccagg catttcgttc
    24481 tgtatagaca taagcattcg tcgtcaaagg gaggaatatt catgatatgc taccactttg
    24541 ggccctggtg gaccagaaaa gggcttgtct cttctcatca gggtagctat agtgtcgccc
    24601 cttcgcagac catgggtcta aagacgaagg cagcgcagtc aatcagcagg aaggtggcat
    24661 gtctgcacaa cccgtcgata tccaaatttt tggccgttca ctgcgtgtga actgcccgcc
    24721 tgaccaaagg gatgcgttga atcaggcagc ggacgatctg aaccaacggt tgcaagatct
    24781 gaaagaacgc actagagtca caaatactga acagttggtc ttcattgccg cattgaatat
    24841 cagctatgag ttagcgcaag aaaaagcgaa gactcgtgac tacgcggcaa gtatggaaca
    24901 gcgtattcgg atgctgcagc agaccataga acaagcgtta cttgaacaag gtcgcatcac
    24961 cgaaaaaact aaccaaaact ttgaatgaca cttttcggtt tactgtggta gagtaaccgt
    25021 gaagacaaaa tttctctgag atgttcgcaa gcgggccagt cccctgagcc gatatttcat
    25081 accacaagaa tgtggcgctc cgcggttggt gagcatgctc ggtccgtccg agaagcctta
    25141 aaactgcgac gacacattca ccttgaacca agggttcaag ggttacagcc tgcggcggca
    25201 tctcggagat tcccttctta tctggcacca gccatgacgc aactaccaga actcccactg
    25261 acattatccc gacaagaaat ccgcaaaatg attcggcaac gtcgtcgtgc gttaacgccg
    25321 gaacaacagc aggaaatggg tcaacaagcc gctacccgga tgatgactta tcccccggtg
    25381 gtgatggcgc atacggtcgc tgtattcctc tcttttgatg gtgaactcga cacccagccg
    25441 ctaatagaac aactctggcg cgccggtaag cgcgtatatc ttccagtttt gcatcccttt
    25501 agtgccggta atttgctgtt cctgaattac catccgcaaa gcgaactggt gatgaacagg
    25561 ttgaagatcc atgagccaaa actggatgtg cgtgacgtgc tacccctttc ccgattagac
    25621 gtgctgatca caccgctggt cgcctttgat gagtacggtc agcgcctggg aatgggcggt
    25681 ggtttttatg atcggacctt acaaaactgg cagcactata aaacgcaacc ggtgggttat
    25741 gcgcatgatt gtcagttggt ggaaaaactc cccgttgaag agtgggatat ccctcttcct
    25801 gcggtggtta caccgtcgaa agtctgggag tggtaagggc gatacacctg catcgccctg
    25861 attgacatcg ttgattcttt aacctaattt agtgagtaag ggtaagggag gattgctcct
    25921 cccctgtgac tgactgttaa taagctggga aacttatgag taacagtaca atcagtatga
    25981 tggcaagtcg catcataacc cttctccttc aagccctcgc ttcggtgagg gctttaccgt
    26041 tacagcccca tgctgcccag ctatcgtaag ctctctttaa ataactacaa cgcattgatc
    26101 tacctttaaa tcttttcttc gagcgtgctc gaaaaaccca tgcaaaacag agataatttt
    26161 tgggaaaaag gatctattac ggcagtaaac gtgggcgatg aagtggctat caaaaagttc
    26221 ggttcagggc gatgcacccc gcatcgccct gattgacatc gttgattctt tgacctaatt
    26281 tagtgagtaa gggtaagaga ggatgactcc tcccctgaga ctgactgtta ataagctccg
    26341 aaacttatca gtaacagtac aatcagtatg atgacaagtc gcatcataac ccttctcctt
    26401 taagccctcg cttcggtgag ggctttaccg ttacagcccc atgctgtccc gccatcgtaa
    26461 atccccatta aataaacaca acgcattgat ctgagtttga tttattttct ggagcagact
    26521 cgcaaagtag aatgcgcaac gcggcaacgg tgtggagaag ggataaaaaa acgggcaagt
    26581 cagtgacctg cccgttaatt ttcagagaag gggaattagt acagcagacg ggcgcgaatg
    26641 gtacccggaa tagctttcat tgcctgcagc gctttttcgg caacgtcttc gtcagcttca
    26701 atatcaataa ctacataacc catctgggcg gaagtttgta gatattgcgc ggcgatgttg
    26761 acgccctgct cggcgaagat tttgttcagc gcagttagca cgcccggacg gttttcgtgg
    26821 atgtgcatca gacgacgacc accatgcagt ggcagcgaga cttccgggaa gttcaccgca
    26881 gagagcgttg agccattgtc agaatacttg atcaatttac ccgcaacttc caggccgata
    26941 ttctcctgcg cttcctgagt cgaaccgcca atgtgtggcg tcagaaggac gttgtcgaat
    27001 tcacacagtg gagaggtaaa tggatcgcta ttggtcgccg gttccgtcgg gaatacgtcg
    27061 attgccgccc ccgccagatg tttgctcgcc agcgcatcac acagcgccgg aatatccacc
    27121 acagtaccgc gcgaggcatt aatcagcagc gagccgggct tcattagcga aatctctttc
    27181 gcgcccatca tatttttggt ggacggattc tctggtacat gcagactcac cacatcgctc
    27241 atattcagca ggtcagaaag atgctgtacc tgagtggcgt tgcccagcgg cagtttgttt
    27301 tcaatatcat aaaagtaaac atacattccc agcgattcag ccagaatgcc caattgcgta
    27361 ccaatatgac cgtagccgat gatacccagc tttttgccgc gcgcttcaaa agaacccgcc
    27421 gccagtttgt tccacacgcc acggtgcgct ttagcattgg cttccggcac gccgcgcaat
    27481 agcagcagca gttcgccaat caccagctcc gcaacagagc gcgtatttga gaacggtgcg
    27541 ttaaataccg ggatcccgcg ctttgccgcc gcatccagat caacctggtt tgttccgata
    27601 cagaaacagc caatagcgac cagtttttct gcggcgttga tcacgtcttc agtcagatgg
    27661 gtacgggatc gcaggccgat gaagtgggca tcgcggatgg attcttttaa ttgttcatca
    27721 tccagcgcgc ctttgtgaaa ttcgatgttg gtgtaaccag ctgcacgaag gctttccagc
    27781 gccttttggt gcacgccttc taccagcaga aacttaatct tgtctttctc cagcgatacc
    27841 tttgccattt acccaatcct gtcttttgaa atgttgtgtg cggatttgca tccgcctttc
    27901 aacatatcaa aaaataatat tgcggcaata tgaacgtttg cgtcgcgatg ttgaggaaat
    27961 atcacgcaaa ggtatttctt ggttgaatat gctggtttag aggatttatt tagagcaatc
    28021 gacaatagcc aagtaaaaac gtgacacatg tcaccaaatt taatgaagag aattttttta
    28081 acgggggagg ttcccccgtc agatcatttc acaatggttt tgacaccgtc aggtgtgcca
    28141 atcagcgcaa cgtccgcgcc acggttagca aacaagccaa cagtcaccac gccaggaatc
    28201 gcatttatgg cgttttccat cgctatcggg tcaaggattt ccatgccgtg gacgtcgagg
    28261 atcacgttgc cgttatcggt caccacgccc tgacggtatt ccggacgacc gcccagtttc
    28321 accagctggc gcgccactgc actacgtgcc atcgggataa cttctactgg cagcgggaat
    28381 ttgcccagaa tatcaacctg cttggaagcg tcagcaatac agataaattt ttccgcaacc
    28441 gaagcaatga ttttttcacg ggtcagcgcc gcgccgccgc ctttgatcat ttgcatgtgg
    28501 ccgttgattt catctgcgcc atcaacgtag atgccaaggc tgtcgacttc gttgagatca
    28561 aaaacgtgaa tgccgaggct tttcagtttt tcagtggaag catctgaact ggaaacggcc
    28621 ccttcaatct ggcctttcat tgtaccgagc gcgtcaataa agtgtgcggc ggtggaacct
    28681 gtacctacac caacaatggt gccgggctga acatactgaa gtgccgccca tcctactgct
    28741 tttttcaatt catcctgcgt catgatcgtt tcgcctgtgg tatgaaattt cacacgcatt
    28801 atatacaaaa aaagcgattc agaccccgtt ggcaagccgc gtggttaact catccataaa
    28861 atatcgcgca atggcgggca tcccctttcg ccccgcaaat aaagcataca acggtctggg
    28921 tatgccgctc cacggtgcga acaggcgcac cagttccccc cgttcgcaag cccctgttta
    28981 caggcaaatt gaggcaataa cgccacccca ttcaacacaa ccagggcacg aactcgctga
    29041 gcgaaatgca ttagcgcaaa tccctgatct tttaagctgc gcgtttaacc acggatacaa
    29101 atgttacccg ccgacggctt cggtatatgc aacctgacac aaaattgtgt catagtgcag
    29161 gaaaaagcat ttaccaggag cagacaacag caatgaaacg cccggactac agaacattac
    29221 aggcactgga tgcggtgata cgtgaacgag gatttgagcg cgcggcacaa aagctgtgca
    29281 ttacacaatc agccgtctca cagcgcatta agcaactgga aaatatgttc gggcagccgc
    29341 tgttggtgcg taccgtaccg ccgcgcccga cggaacaagg gcaaaaactg ctggcactgc
    29401 tgcgccaggt ggagttgctg gaagaagagt ggctgggcga tgaacaaacc ggttcgactc
    29461 cgctgctgct ttcactggcg gtcaacgccg acagtctggc gacgtggttg cttccggcac
    29521 tggctcctgt gttggctgat tcgcctatcc gcctcaactt gcaggtagaa gatgaaaccc
    29581 gtactcagga acgtctgcgc cgcggcgaag tggtcggcgc ggtgagtatt caacatcagg
    29641 cgctgccgag ttgtcttgtc gataaacttg gtgcgctcga ctatctgttc gtcagctcaa
    29701 aaccctttgc cgaaaaatat ttccctaacg gcgtaacgcg ttcggcatta ctgaaagcac
    29761 ctgtggtcgc ctttgaccat cttgacgata tgcaccaggc ctttttgcag caaaacttcg
    29821 atctgcctcc aggcagcgtg ccctgccata tcgttaattc ttcagaagcg ttcgtgcaac
    29881 ttgctcgcca gggcaccacc tgctgtatga tcccgcacct gcaaatcgag aaagaactag
    29941 ccagcggtga actgattgac ttaacgccag ggctatttca acgccggatg ctctactggc
    30001 accgctttgc tcctgaaagc cgcatgatgc gtaaagtcac tgatgcgttg ctcgattatg
    30061 gtcacaaagt ccttcgtcag gattaatcca tcaaataatg cctgatagca catatcaggc
    30121 gttatcctca cttctttttg tattccttga atcacatcac aaaatagaca aatctcaggc
    30181 ggcaaaaaac gacgtctgaa tgcatttttt tgctggcgac aaacccacgt aaaaagctca
    30241 ccgtaagcgc aaataccctc attttgattg cgttttacgg agcaaataat gtctaacgag
    30301 caggagtggc aacagcttgc caacaaggaa ttgagccgtc gggagaaaac tgtcgactcg
    30361 ctggttcagc aaaccgcgga agggatcgcc atcaagcagc tgtataccga agccgatctc
    30421 gataatctgg aggtgacagg tacccttcct ggtttgccgc cctacgttcg tggcccgcgt
    30481 gccactatgt ataccgccca accgtggacc atccgtcagt atgctggttt ttcaacagca
    30541 aaagagtcca acgcttttta tcgccgtaac ctggccgccg ggcaaaaagg tctttccgtt
    30601 gcgtttgacc ttgccaccca ccgtggctac gactccgata acccacgcgt ggcgggtgac
    30661 gtcggcaaag cgggcgtcgc tatcgacacc gtggaagata tgaaagtcct gttcgaccag
    30721 atcccgctgg ataaaatgtc ggtttcgatg accatgaatg gcgcagtgct accagtactg
    30781 gcgttttata tcgtcgccgc agaagagcaa ggtgttacac ctgataaact gaccggcacc
    30841 attcaaaacg atattctcaa agagtacctc tgccgcaaca cctatattta cccaccaaaa
    30901 ccgtcaatgc gcattatcgc cgacatcatc gcctggtgtt ccggcaacat gccgcgattt
    30961 aataccatca gtatcagcgg ttaccacatg ggggaagcgg gtgccaactg cgtgcagcag
    31021 gtagcattta cgctcgccga tgggattgag tacatcaaag cagcaatctc tgccggactg
    31081 aaaattgatg acttcgctcc tcgcctgtcg ttcttcttcg gtatcggcat ggatctgttt
    31141 atgaacgtcg ccatgttgcg tgcggcacgt tatttatgga gcgaagcggt cagtggattt
    31201 ggcgcacagg atccgaaatc actggcgctg cgtacccact gccagacctc aggctggagc
    31261 ctgactgaac aggatccgta taacaacgtt atccgcacca ccattgaagc actggctgcg
    31321 acgctgggcg gtactcagtc actgcatacc aacgcctttg acgaagcgct tggtttgcct
    31381 accgatttct cagcacgcat tgcccgcaac acccagatca tcatccagga agaatcagaa
    31441 ctctgccgca ccgtcgatcc actggccgga tcctattaca ttgagtcgct gaccgatcaa
    31501 atcgtcaaac aagccagagc tattatccaa cagatcgacg aagccggtgg catggcgaaa
    31561 gcgatcgaag caggtctgcc aaaacgaatg atcgaagagg cctcagcgcg cgaacagtcg
    31621 ctgatcgacc aaggcaagcg tgtcatcgtt ggtgtcaaca agtacaaact ggatcacgaa
    31681 gacgaaaccg atgtacttga gatcgacaac gtgatggtgc gtaacgagca aattgcttcg
    31741 ctggaacgca ttcgcgccac ccgtgatgat gccgccgtaa ccgccgcgtt gaacgccctg
    31801 actcacgccg cacagcataa cgaaaacctg ctggctgccg ctgttaatgc cgctcgcgtt
    31861 cgcgccaccc tgggtgaaat ttccgatgcg ctggaagtcg ctttcgaccg ttatctggtg
    31921 ccaagccagt gtgttaccgg cgtgattgcg caaagctatc atcagtctga gaaatcggcc
    31981 tccgagttcg atgccattgt tgcgcaaacg gagcagttcc ttgccgacaa tggtcgtcgc
    32041 ccgcgcattc tgatcgctaa gatgggccag gatggacacg atcgcggcgc gaaagtgatc
    32101 gccagcgcct attccgatct cggtttcgac gtagatttaa gcccgatgtt ctctacacct
    32161 gaagagatcg cccgcctggc cgtagaaaac gacgttcacg tagtgggcgc atcctcactg
    32221 gctgccgatc ataaaacgct gatcccggaa ctggtcgaag cgctgaaaaa atggggacgc
    32281 gaagatatct gcgtggtcgc gggtggcgtc attccgccgc aggattacgc cttcctgcaa
    32341 gagcgcggcg tggcggcgat ttatggtcca ggtacaccta tgctcgacag tgtgcgcgac
    32401 gtactgaatc tgataagcca gcatcatgat taatgaagcc acgctggcag aaagtattcg
    32461 ccgcttacgt cagggtgagc gtgccacgct cgcccaggcc atgacgctgg tggaaagccg
    32521 tcacccacgt catcaggcgc taagtacaca gctgcttgac gccattatgc cgttctgtgg
    32581 taacgccctg cgactgggcg ttaccggcac acccggcgcg gggaaaagta cctttcttga
    32641 agcctttggc atgttgttga ttcgtgaggg attaaaggtt gctgttattg cggtcgatcc
    32701 cagcagcccg gtcactggcg gtagcattct cggggataaa acccgcatga atgacctggc
    32761 gcgtgccgag gcggcgttta ttcgcccggt accctcctcc ggtcatctgg gcggtgccag
    32821 tcagcgagca cgggaattaa tgctgttatg cgaagcggcg ggttatgacg tagtgattgt
    32881 cgaaacggtt ggcgtcgggc agtcggaaac ggaagtcgcc cgcatggtgg actgttttat
    32941 ctcgttgcaa attgccggtg gcggcgatga tctgcagggc attaaaaaag ggctgatgga
    33001 agtggctgat ctgctcgtta tcaacaaaga cgatggcgat aaccatacca atgtcgccat
    33061 tgcccggcat atgtacgaga gtgccctgca tattctgcga cgtaaatacg acgaatggca
    33121 gccacgggtt ctgacttgta gcgcactgga aaaacgtgga atcgatgaga tctggcacgc
    33181 catcatcgac ttcaaaaccg cgctaactgc cagtggtcgt ttacaacaag tgcggcaaca
    33241 acaatcggtg gaatggctgc gtaagcagac cgaagaagaa gtactgaatc acctgttcgc
    33301 gaatgaagat ttcgatcgct attaccgcca gacgctttta gcggtcaaaa acaatacgct
    33361 ctcaccgcgc accggcctgc ggcagctcag tgaatttatc cagacgcaat attttgatta
    33421 aaggaatttt tatgtcttat cagtatgtga acgttgccac tatcaacaaa gtggcggtca
    33481 ttgagtttaa ctatggacga aaacttaatg ccttaagtaa agtctttatt gatgatctta
    33541 tgcaggcgtt aagcgatctc aaccggccgg aaattcgctg tatcattttg cgggcgccaa
    33601 gtggatccaa agtcttctcc gcaggtcacg atattcacga actgccgtct ggcggtcgcg
    33661 atccgctctc ctatgatgat ccattgcgtc aaatcacccg catgatccaa aaattcccga
    33721 aaccgatcat ttcgatggtg gaaggtagtg tttggggtgg cgcatttgaa atgatcatga
    33781 gttccgatct gatcatcgcc gccagtacct caaccttctc aatgatgcct gtaaacctcg
    33841 gcgtcccgta taacctggtc ggcattcaca acctgacccg cgacgcgggc ttccacattg
    33901 tcaaagagct gatttttacc gcttcgccaa tcaccgccca gcgcgcgttg gctgtcggca
    33961 tcctcaacca tgttgtggaa gtggaagaac tggaagattt caccttacaa atggcgcacc
    34021 acatctctga gaaagcgccg ttagccattg ctgttatcaa agaagagctg cgtgtactgg
    34081 gcgaagcgca caccatgaac tccgatgaat ttgaacgtat tcaggggatg cgccgcgcgg
    34141 tttatgacag cgaagattac caggaaggga tgaacgcttt cctcgaaaaa cgtaaaccta
    34201 atttcgttgg tcattaatcc ctgcgaacga aggagtaaaa atggaaactc agtggacaag
    34261 gatgaccgcc gatgaagcgg cagaaattat ccagcataac gacatggtgg catttagcgg
    34321 ctttacccca gcgggttcgc cgaaagcctt accgaccgcg attgcccgca gagctaacga
    34381 acagcatgag gccaaaaagc cgtatcaaat tcgcctgctg acgggggcgt caatcagcgc
    34441 cgccgctgac gatgtacttt ctgacgccga tgctgtttcc tggcgtgcgc catatcaaac
    34501 atcgtccggt ttacgtaaaa agatcaatca gggcgcggtg agtttcgttg acctgcattt
    34561 gagcgaagtg gcgcaaatgg tcaattacgg tttcttcggc gacattgatg ttgccgtcat
    34621 cgaagcatcg gcactggcac cggatggtcg agtctggtta accagcggga tcggtaatgc
    34681 gccgacatgg ctgctgcggg cgaagaaagt gatcattgaa cttaatcact atcacgatcc
    34741 gcgcgttgca gaactggcgg atattgtgat tcctggcgca ccaccgcggc gcaatagcgt
    34801 gtcgatcttc catgcaatgg atcgcgtcgg tacccgctat gtgcaaatcg atccgaaaaa
    34861 gattgtcgcc gtcgtggaaa ccaacttgcc cgacgccggt aatatgctgg ataagcaaaa
    34921 tcccatgtgc cagcagattg ccgataacgt ggtcacgttc ttattgcagg aaatggcgca
    34981 tgggcgtatt ccgccggaat ttctgccgct gcaaagtggc gtgggcaata tcaataatgc
    35041 ggtaatggcg cgtctggggg aaaacccgga aattcctccg ttcatgatgt attcggaagt
    35101 gctacaggaa tcggtggtgc atttactgga aaccggcaaa atcagcgggg ccagcgcctc
    35161 cagcctgaca atctcggccg attccctgcg caagatttac gacaatatgg attactttgc
    35221 cagccgcatt gtgttgcgtc cgcaggagat ttccaataac ccggaaatca tccgtcgtct
    35281 gggcgtcatc gctctgaacg tcggcctgga gtttgatatt tacgggcatg caaattcaac
    35341 gcacgtagcc ggggtcgatc tgatgaacgg catcggcggc agcggtgatt ttgaacgcaa
    35401 cgcgtatctg tcgatcttta tggctccgtc gattgctaaa gaaggcaaga tctcaaccgt
    35461 cgtgccaatg tgcagccatg ttgatcacag cgaacacagc gtcaaagtga tcatcaccga
    35521 acaagggatc gccgatctgc gcggtctttc cccgcttcaa cgcgcccgca ctatcattga
    35581 taattgtgca catcctatgt atcgggatta tctgcatcgc tatctggaaa atgcgcccgg
    35641 cggacatatt caccacgatc ttagccacgt cttcgactta caccgtaatt taattgcaac
    35701 cggctcgatg ctgggttaat ggtttgtccc gtcacgatct tcagcaatat gctgaagcat
    35761 cgtggcggta taacgatgat ttctgattga ataaagatat tcctgcatat acatcggcct
    35821 gtgcggcgca tcaaaatatt tcagcgttgc cggattcacc agtcgccagg caaaatgcgg
    35881 gatcaccgtc agaaaatgtc cgcgctccac ggcgctgatt ttggccataa aactgtaggg
    35941 acgataaata atggtgggat tgatcccaca aggtcgcata ttcgcatcaa tcattgcttc
    36001 gaagttagcc cggttttgaa agcgcatttg tagccagggc agctcctgaa gcagctcttg
    36061 ctcttgacgc tcttcataac ggcgagaaac gagaaagccc aatcgcagcg gtggtaattc
    36121 actgatagtc aggttttcca gttcctgaac tcgtgcagat acatgctgtg gggagataat
    36181 aaaatctaac tgacggtcaa aaagattatc gataacgccg ttttcgctga actcaactgg
    36241 ctgggcggtc acgccttcat acttatctcc gagactaatc aactgatcga aaataattgt
    36301 cggataagta ttatctatgc caatcacaat t
//
LOCUS       DAEEKQ010000025        32432 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.025, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000025 DAEEKQ010000000
VERSION     DAEEKQ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.025"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..611
                     /locus_tag="KLK57_001213"
                     /pseudo
     CDS             1..611
                     /locus_tag="KLK57_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
     gene            608..1222
                     /locus_tag="KLK57_001214"
     CDS             608..1222
                     /locus_tag="KLK57_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HBE0891640.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALERLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="KLK57_001215"
     CDS             complement(1279..2736)
                     /gene="pepD"
                     /locus_tag="KLK57_001215"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HBE0891641.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            2997..3455
                     /gene="gpt"
                     /locus_tag="KLK57_001216"
     CDS             2997..3455
                     /gene="gpt"
                     /locus_tag="KLK57_001216"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBE0891642.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            3547..4791
                     /gene="frsA"
                     /locus_tag="KLK57_001217"
     CDS             3547..4791
                     /gene="frsA"
                     /locus_tag="KLK57_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBE0891643.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            4849..5250
                     /gene="crl"
                     /locus_tag="KLK57_001218"
     CDS             4849..5250
                     /gene="crl"
                     /locus_tag="KLK57_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBE0891644.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="KLK57_001219"
     CDS             complement(5289..6344)
                     /gene="phoE"
                     /locus_tag="KLK57_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HBE0891645.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            6632..7735
                     /gene="proB"
                     /locus_tag="KLK57_001220"
     CDS             6632..7735
                     /gene="proB"
                     /locus_tag="KLK57_001220"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HBE0891646.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            7747..9000
                     /gene="proA"
                     /locus_tag="KLK57_001221"
     CDS             7747..9000
                     /gene="proA"
                     /locus_tag="KLK57_001221"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HBE0891647.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            9115..9190
                     /locus_tag="KLK57_001222"
     tRNA            9115..9190
                     /locus_tag="KLK57_001222"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9148..9150,aa:Thr,seq:cgt)
     gene            complement(9205..10368)
                     /locus_tag="KLK57_001223"
     CDS             complement(9205..10368)
                     /locus_tag="KLK57_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE0891648.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWKLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNLSQSKNKEGTNDV"
     gene            complement(10595..10900)
                     /locus_tag="KLK57_001224"
     CDS             complement(10595..10900)
                     /locus_tag="KLK57_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891649.1"
                     /translation="MTTFTDKELIKEIKERISSLDVRDDIERRAYEIALLSLEVEPDE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRTGIHFSTMSQQEVK"
     gene            complement(10900..11262)
                     /locus_tag="KLK57_001225"
     CDS             complement(10900..11262)
                     /locus_tag="KLK57_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891650.1"
                     /translation="MRMNVFEMEGFLRGRCVPRDLKVNETDVEYLVRKFDALEAKCAA
                     QENKVIPVSTELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            complement(11253..11789)
                     /gene="yfdR"
                     /locus_tag="KLK57_001226"
     CDS             complement(11253..11789)
                     /gene="yfdR"
                     /locus_tag="KLK57_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBE0891651.1"
                     /translation="MSFIKTFSGKHFYYDRINKDDIVINDIAVSLSNICRFAGHLSHF
                     YSVAQHAALCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFKVIPQAPGHA
                     YGMFMERFNELSELRKCA"
     gene            12431..12925
                     /locus_tag="KLK57_001227"
     CDS             12431..12925
                     /locus_tag="KLK57_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891652.1"
                     /translation="MTRDDYKFYIHYSYVLEKMNYTLLTRIDKLITLMLIVLGFSVFA
                     PYSNLFIFGLFVAVLSVLQLVYQFGQEAGMSKEQARHYKRLLIESDTLSEDQLQARYL
                     KIQDTDSNPWKSLERAAFIRACIFAGVPTKYDLTTKEKFIAWCAGDLPLENELTDIND
                     DQQA"
     gene            complement(13268..13942)
                     /locus_tag="KLK57_001228"
     CDS             complement(13268..13942)
                     /locus_tag="KLK57_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional repressor"
                     /protein_id="HBE0891653.1"
                     /translation="MKTIHDIRRSNARKLRDGVGGNSSFATMIDREPTQTSRFMGDGA
                     TKNIGDSMARHIEKCFDLPVGWLDQEHQTTNITKKPDVSITNKQITLVPVISWVQAGA
                     WKEVGYSEVDLSTAETYPCPVPCGEMTYILRVIGDSMIDEYRPGDMIFVDPEVPACHG
                     DDVIALMHDTGETTFKRLIEDGTQRYLKALNPNWPEPYIKINGNCSIIGTVIFSGKPR
                     RYKIKA"
     gene            14033..14233
                     /locus_tag="KLK57_001229"
     CDS             14033..14233
                     /locus_tag="KLK57_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0891654.1"
                     /translation="MKAYWDSLTKEQQGELAGKVGSTPGYLRLVFNGYKKASFVLAKK
                     LEQCTSGAITKSDLRPDIYPKD"
     gene            14277..14828
                     /locus_tag="KLK57_001230"
     CDS             14277..14828
                     /locus_tag="KLK57_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891655.1"
                     /translation="MGKYHWKVEKQPEWYVKAVRKTIAALPGGYAEAADWLDVTENAL
                     FNRLRADGDQIFPLGWAMVLQRAAGTHYIADAVAQSAGGVFVSLPEIEEVENADINQR
                     LLEVIEQIGNYSKQIRSAIEDGVVEPHEQTAINDELYLSISKLQEHAALVYKIFCAPE
                     KSDARECAAPGVVAFCVCGETNA"
     gene            14825..15661
                     /locus_tag="KLK57_001231"
     CDS             14825..15661
                     /locus_tag="KLK57_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001376881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBE0891656.1"
                     /translation="MNSLTANNRLSQQLVVSVAEHLLLRHECRLPNHLAVSNHRELYL
                     TVGGELCRNLTAGFVTEEDFMFMLFVGSQKHSALSIFAKTTRMSALVLCGNSGVILLS
                     VKDHQHIDSAIPGRYTVQAPYKAGAGIGVLELIKAIYDAPASFLSSAFAHIKVMVGWA
                     GASKDAPVSLITGYANPVQFTTSEIGVSGGGSISPIKEAAIMATVPALTRLNDEDLHK
                     LSYVTTALRALRKVTLSDPQAHQVLVETLLNLQAERIRLADKANFHIHRLLNISGGHR
                     HA"
     gene            15654..15890
                     /locus_tag="KLK57_001232"
     CDS             15654..15890
                     /locus_tag="KLK57_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000933940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891657.1"
                     /translation="MLNPLILNICRLLQRKKTSIPTVGQWYTTPAGHVLRVSLVDREC
                     QKVICEPLGRNYRVSMPLIAFRSGKNMKHLGGAA"
     gene            15887..16705
                     /locus_tag="KLK57_001233"
     CDS             15887..16705
                     /locus_tag="KLK57_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0891658.1"
                     /translation="MSMELMVKAMKIRVGNPLRKLVLIKLADNASDQGECWPSYQHIA
                     DQCEISKRSVMNHIAALCESGLVKKVTRKGEKGNSSNIYLLHLDGAGDSLGGSANNSL
                     SGAANSPGSAGVAPGGSAGDSPRTSHSFEPVKEPVNEPIAVGASVDESVRVRSNRPEY
                     SPEFEQAWLAYPKRAGGNSKSAAFKAWKARLNEGVNPETMLEGVKRYAGWVSAMGNSG
                     TQFVKQAVTFFGPDRHFEESWEVPAVSAARREDPYFKSSYDNVDYSQIPAGFRG"
     gene            16702..17196
                     /locus_tag="KLK57_001234"
     CDS             16702..17196
                     /locus_tag="KLK57_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HBE0891659.1"
                     /translation="MIMSLLNDVQKFIEAHPGCTSGDIADAFAGYSRQRVLQSASKLR
                     QSGRVAHRCEGDTRRHFPRLTERAQEPEPQPVRETRPVRNFYIGTNDPRVILCLTRQA
                     EELESRGLYRRAATVWMAAFRESHSQPERNNFLARRERCLRKSSKRAASGEEWYLSGN
                     YVGA"
     gene            17196..17849
                     /locus_tag="KLK57_001235"
     CDS             17196..17849
                     /locus_tag="KLK57_001235"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001378271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HBE0891660.1"
                     /translation="MSNKYCQALVELRNKPAHELKDVGDQWRTPDNIFWGINTLFGPF
                     VLDLFTDGDNAKCAAYYTAEDNALAHDWSERLAELKGAAFGNPPYSRASQHEGQYITG
                     MRYIMKHASAMRDKGGRYVFLIKAATSEVWWPEDADHIAFIRGRIGFELPVWFIPKDE
                     KQVPTGAFFAGAIAVFDKTWKGPAISYIGRDELEACGEAFLAQVRQQAEKLVREMAA"
     gene            17846..18172
                     /locus_tag="KLK57_001236"
     CDS             17846..18172
                     /locus_tag="KLK57_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA family transcriptional regulator"
                     /protein_id="HBE0891661.1"
                     /translation="MTTLTQCQQQVLDMLISYQKERGFPPTNQEVATMLGYRSVNAAV
                     EHLRALEKKGVITIKRGVARGITLHTAVKDDDSEAVGIIRALLAGEENARLRATHWLH
                     ERGLKA"
     gene            18169..18558
                     /locus_tag="KLK57_001237"
     CDS             18169..18558
                     /locus_tag="KLK57_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBE0891662.1"
                     /translation="MKLILPFPPSVNTYWRHPNKGAFAGKSLISAAGRKFQSAACAAI
                     VEQLRRLPKPTSAPASVEIVLFPPDNRIRDLDNYNKALFDALTHAGVWEDDRQVKRML
                     VEWGPVIPKGKVEITISKYEKPAGAAA"
     gene            18578..19387
                     /locus_tag="KLK57_001238"
     CDS             18578..19387
                     /locus_tag="KLK57_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HBE0891663.1"
                     /translation="MNNLMVIDGIEVRRDVYGRYSLNDLHRAAVASGANARTKEPGKF
                     LSSQQTVELVHELTNTQNLGVDPVSVIHGGNERGTYVCKELVYAYAMWISPSFHLKVI
                     RTFDMVTSTPEKLSGQAADKMQAGVILLDFMRRELNLSNSSVLGACQKLQEAVGLPNL
                     APRYAIDAPADAHDGSSRPTLSLSALLKQYGIRLTANQAYHQMVKLGIVEQRERYSRT
                     AINNIKKFWSLTAKGCMFGKNITSPANPRETQPHFFESRFPELLKLLDTVH"
     gene            19395..20384
                     /locus_tag="KLK57_001239"
     CDS             19395..20384
                     /locus_tag="KLK57_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBE0891664.1"
                     /translation="MRALLTPEIAPRMGIVLFRPGSELMPLFMQGRVLLEPEPERYSS
                     FASGAVPAASQPLADDPAVRAVFRNEAVIRRAGGVECLESWLLREKGCQWPHSNWHSE
                     NMTTMRHAPGAIRLCWHCDNQLRDQFTERLESMATDNCARWVLSVVRRDLGFDDSHVV
                     TMPELCWWLIRNDLADALPESAARKALRLPKPVVPSVTRESDLVPSVPATSIIQDKAK
                     KVLALKVDPESPESFMLRPKRRRWVNEKYTRWVKTQPCACCGKPADDPHHLIGHGQGG
                     MGTKAHDLFVLPLCRKHHDELHADTVAFEEKYGSQLELIFRFIDRALAIGVLA"
     gene            20398..21150
                     /locus_tag="KLK57_001240"
     CDS             20398..21150
                     /locus_tag="KLK57_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBE0891665.1"
                     /translation="MNLEALPKYYSPKSPKLSDDAPATGSGGLTITDVMAAQGMVQSK
                     APLGFALFLAKVGVQDPQFAIEGLLNYAMALDNPTLNKLSEETRLQIIPYLVNFAFAD
                     YSRSAASKARCEHCAGTGFHNVLREVVKHSRSGESVIKEEWVKELCQHCHGKGEVSTA
                     CRGCKGKGIVLDEKRTRLHGTPVYKVCGRCNGNRFSRLPTTLARLHVQKLVPDLTDYQ
                     WYKGYADVIDKLVTKCWQEEAYAEIQLRKVTR"
     gene            21404..21607
                     /locus_tag="KLK57_001241"
     CDS             21404..21607
                     /locus_tag="KLK57_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891666.1"
                     /translation="MLKQQDMTETAAAVLHFLPADKWVTPRMMTRTTGVSEARCQLIL
                     TQLVLAGLAKDNGGYGNKFRRCQ"
     gene            21758..22810
                     /locus_tag="KLK57_001242"
     CDS             21758..22810
                     /locus_tag="KLK57_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBE0891667.1"
                     /translation="MKNTVKINSVDLINADCLHFIQSLPDDSIDLIVTDPPYFKVKPN
                     GWDNQWKGDEDYLKWLDHCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFNVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERVLFAEHYQGPYRGKSDGYAAKERELKQHIMA
                     PLISYFRDARAELGITAKQIAEATGKKNMVSHWFGASQWQLPNEADYRKLQALFSRIA
                     AEKFQEQQLEQPHHQLVASYDSLNRKYSELLDEFKSLRRYFSVSVSVPYTDVWMHKPV
                     QFYPGKHPCEKPADMLRQIINASSRPGDLVADFFMGSGSTIKAAMALGRRALGVELES
                     ERFNQTVKEINELVGK"
     gene            22878..23093
                     /gene="essD"
                     /locus_tag="KLK57_001243"
     CDS             22878..23093
                     /gene="essD"
                     /locus_tag="KLK57_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBE0891668.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
     gene            23093..23590
                     /gene="rrrD"
                     /locus_tag="KLK57_001244"
     CDS             23093..23590
                     /gene="rrrD"
                     /locus_tag="KLK57_001244"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBE0891669.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPSGDDGLEGVSYIPYKD
                     IVGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWIYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            23587..24030
                     /locus_tag="KLK57_001245"
     CDS             23587..24030
                     /locus_tag="KLK57_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBE0891670.1"
                     /translation="MSRVTTIISALIICIIVCLLWAVNHYRDNAIAYKEQRDKAASTI
                     ADMQKRQRDVAELDARYTKELADANATIESLRADVSAGRKWLRVKAVCPDMHKITAAS
                     GVDDGTSPRLTDTAQRDYFTLRKRIETSDKMIRGLQQYIRTQCVR"
     gene            complement(24411..24731)
                     /locus_tag="KLK57_001246"
     CDS             complement(24411..24731)
                     /locus_tag="KLK57_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891671.1"
                     /translation="MSIKVFRSDLQTESESDYRNWLGDNPDGYVVNALKRTRGKGNKS
                     DERFTRIHRASCKTINPLKRKIDKTGFTTGDYQKICSTNFINVENEAKKMTGLTSIIK
                     CRCI"
     gene            25066..25560
                     /locus_tag="KLK57_001247"
     CDS             25066..25560
                     /locus_tag="KLK57_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HBE0891672.1"
                     /translation="MDRELKNLTLNISQLAALSGVHRQTAAARLQNLPVAGGHESNLK
                     LYRVVDIVSAFLALPPPVAEGEMDAHERKAWYQSERERLKFEQETAQLIPASDVRREF
                     AIWAKAVVQVLETLPDILERDCGLQPVAVSRVQSIIDDLRDQIALRVTEAGADDEEEL
                     QQEE"
     gene            25560..26552
                     /locus_tag="KLK57_001248"
                     /pseudo
     CDS             25560..26552
                     /locus_tag="KLK57_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            26717..27955
                     /locus_tag="KLK57_001249"
     CDS             26717..27955
                     /locus_tag="KLK57_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0891673.1"
                     /translation="MPVKPLTVTEVKGMKPREKDYAVYDGFGLLLNVSKAGGKVWRFR
                     YSHPITKKRQTYTIGRFPEFSLAEAREIRDELRRMIARGVDPVTEKKNRKIEMSLKNL
                     QTFEAVANAWIAFKKGSELRKPTLYNIEYEVYKYLVPFFGKYSIEKITAPVAINALDA
                     VSDKNALQKKLISRLNEIMNYAVNCGALKTNPLLKIKTAFTGKKNKSLAALPVERLPE
                     FLSWWDSVPHKYQIAHNALLFQILTMVRPGEAIKAEWSEIDFDSGLWIIPAHKMKCHR
                     EHVVPLSSQAIRILRTMQKIKRGRYIFFSSRTKDAPMGKNTINTPIAASKFKGIVTLH
                     GFRSMWSTLLNEEGFNPDVIEAALAHKSGDKIRDIYNRTTYLEQRKIMMQWVGDFFDE
                     ARKGVINRSGGKKGLRIVNG"
     gene            27968..28171
                     /locus_tag="KLK57_001250"
     CDS             27968..28171
                     /locus_tag="KLK57_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891674.1"
                     /translation="MNTNEDILFTKDVMKILRYGAMSAFINFWKDENNGFPQPFRIGR
                     RHTWHRRDVEAWLDKQREQANPH"
     gene            28230..28811
                     /locus_tag="KLK57_001251"
     CDS             28230..28811
                     /locus_tag="KLK57_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirepressor protein"
                     /protein_id="HBE0891675.1"
                     /translation="MNKNIAVTGKGYARSVKKFCDIRDLVVLRFDGVDVRVVYLNGDP
                     WFVAKDVCEALEISNSRDALKALDVDEKNTVALSYGIRGNPNHSVVSESGFYKLIARS
                     RKAVTPGTFAHRFSNWVFRNVIPGIRKTGAYGIPWGALQDFSRRKEQYQISASEKGRE
                     LQACKRKKRELEEEEKRLIREYQPEFYFGERIQ"
     gene            complement(29028..29471)
                     /locus_tag="KLK57_001252"
     CDS             complement(29028..29471)
                     /locus_tag="KLK57_001252"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891676.1"
                     /translation="MKRFRTILLKILGAKTSIAILCVFCGASFLFGQYLNDMNLFASS
                     GAIISVFGLLYTIKFTTLKKLSNREAEINSRSGVTGPPLSAEESQKIREENLAKARVE
                     VREEIKAEVLGVGLTVFCTIIWAYGGYIKIIPSLVMWIRHSLLMQ"
     gene            complement(29503..29697)
                     /locus_tag="KLK57_001253"
     CDS             complement(29503..29697)
                     /locus_tag="KLK57_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system HicB family antitoxin"
                     /protein_id="HBE0891677.1"
                     /translation="MSTIIKRDKKPKGTGKAPPFHMRIAPELKEQFDSEANNDGVSLA
                     NWLKELGRSELKRRGIEPKG"
     gene            29771..30040
                     /locus_tag="KLK57_001254"
                     /pseudo
     CDS             29771..30040
                     /locus_tag="KLK57_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013225.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
     gene            30033..30353
                     /locus_tag="KLK57_001255"
     CDS             30033..30353
                     /locus_tag="KLK57_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891678.1"
                     /translation="MPDMSNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEDYDEKAMRIDMGNIGLLLAMLGHFDISLRCTIENATDALNAIKKANTD
                     SNRG"
     gene            30360..30659
                     /locus_tag="KLK57_001256"
     CDS             30360..30659
                     /locus_tag="KLK57_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBE0891679.1"
                     /translation="MRTYLSGLTASGYAHPQIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFLSSLNVEIFICRFRREIPTSRNNHVSRVDVDKKLQELKNMIAAWREQK"
     gene            30656..>32432
                     /locus_tag="KLK57_001257"
     CDS             30656..>32432
                     /locus_tag="KLK57_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBE0891680.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGSDAWAHAKQWQEQDGRLAGDN
                     VPPVWLGEQQLAELDNLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERGELTEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFRQHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAALFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDS
                     MLAEKIEGELAVVIRHLLTRFADQDEARRLLYEQQKSEEALAIKREGDSLVDFCGYLM
                     ASVMCDGLLVGNAEIVPFSPRRYLYHAYLAYMKAHGFGKPVTLTRFGKDMPGAMAEYG
                     REYMKRKTKHGLRSNVTLTEESEDWM"
BASE COUNT         8274 a         7407 c         8408 g         8343 t
ORIGIN      
        1 ctattttaca gcggcatatt gcctcgtttt cgcatcatgg tttgcctgaa ggcagtgacg
       61 acgcgctacg gtatattgcg gaacatgacg atgcgctggc gtttgcgcgt attaatcgcc
      121 agctgatctc tttacgcata atgcaacagg ttaaggccac cggaagtccg gttctggatg
      181 tggcgcataa ctttgttagc gcgtgtcaaa tcggcgatca acagggctgg ttgcatcgta
      241 aaggtgccac gccggatgac aacggtctgg tgattattcc cggttcactc ggtgattact
      301 cctagcttgt acagcctgtt gcgaacgaaa aaacgctcca ctcgctggcg catggggctg
      361 ggcgtaaatg ggggcgcacc gagtgtaaag ggcgtctggc agcgaaatac acagcgacgc
      421 agctctcacg tactgaactt ggcagtcggg taatttgtcg cgataaacaa ctcatctttg
      481 aagaagcgcc acaagcctat aaatcggctg aaagcgtggt gcaatgtctg gtgcaggctg
      541 gtttaattat tcctgtcgcg cggctgcgtc cggtgctaac gctcaaaaac agtggaggga
      601 aaaaaggatg atcttgctac aactctcctc agcgcagggg ccggaagaat gttgtctcgc
      661 agtgaaaaag gcgctggagc gattaattaa agaagctgcc cgacaagacg tcgcggtaac
      721 ggtgttggaa acagaaacgg gccgctactc tgacacgctg cgttcagctc tggtttctct
      781 ggatggcgat aacgcatggg cattaagcga aagctggtgc ggcactattc agtggatttg
      841 tccgagtccg tatcggcctc atcatgggcg caaaaactgg tttctgggca ttgggcgttt
      901 taccgctgat gagcaggaac aatcggatgc aatccgttat gagacgttgc gttcgtcggg
      961 gccgggcggt caacatgtca ataaaaccga ctcggcggta cgcgccacgc atttggcatc
     1021 cggtattagc gtgaaggttc agtcagagcg tagtcagcat gctaacaagc ggctggcacg
     1081 attgctgatt gcctggaagc tggagcagca gcaacaggaa aatagcgcgg tgctgaaatc
     1141 gcagcggcga atgttccatc accagattga acgtggcaac ccgagacgga catttacagg
     1201 gatggctttt atcgaaggat aatgaaggat gaaactgccg gaaggcgatt aaacgccatc
     1261 cggcagcaaa tcaaataatt acttcgccgg aatttctttc agcagttcag tcagcagtgt
     1321 ccagtaatga cctacgcttt cgatgtgaac ttgctcatcc ggagagtgtg gaccggtgat
     1381 agttggcccg atagaaacca tgtccatttc cggatacggt tttttgaaca gaccacattc
     1441 caggcccgcg tggataatct ggatgttcgg cgttttgttg aacagacgct gataggtttc
     1501 acgtaccaga tgcatcaccg gagaattagc gtccggctgc cagccaggat atgcgccttt
     1561 cgcttcggtt ttcgcgccag ccagtttacc cagcgaatcc agcatgctca ccacgtagtc
     1621 tttaccgctg tcgatcagag aacggatcag acagtgaatt tctacgttat tatcagtcat
     1681 ggtcaccaca ccgacgttca gggaggtttc gaccacacct tttgccacgt ctgagttgcg
     1741 gatcacaccg ttcggggtgg cgttcagcag acgaataaag gtatcgcgag atttcgcaat
     1801 cagggcagct ttatcgttcg ctacagagtc cagcaacaag gccagatttt tctctttctc
     1861 tgccagctcg tttttcagga tctcctgata ggtattcacc agagatttca ggacgtcgac
     1921 tttatcagct gcgacagcaa tggtcgcaaa ggcttcacgc gggatggcgt tacgcagtgt
     1981 gccgccgttg aaatcgataa ggcgcagatc cagttcttct gcatgacccg ctaggaagcg
     2041 caccagcagt ttgttggcat tacccaggcc aacgtggatt tccccgccgg agtgaccccc
     2101 tttcagacct tttaaggtta acttgaaggt ttcaaaacca gctggaaccg cttcacgatc
     2161 taaatgcagg ttggaggtga agtcgatacc ccccgcacaa cccatgtaga tttcaccttc
     2221 ttcttcggag tcggtgttaa tcagaatatc agcctgcaac cagttgctct gtaagccgaa
     2281 cgcaccgtcc ataccggctt cttcggtcat ggtcagcagc acttccagcg ggccgtgaac
     2341 cacgttttcg tcagccagaa ccgccagcgc agacgccata ccaatgccgt tatctgcacc
     2401 cagcgtggtg ccgcgcgctt taacccattc gccatcaata taaggctgga taggatcttt
     2461 cgtgaagtca tgcacggtgt cgttattttt ctgcggcacc atatcgaggt gggcctgtaa
     2521 gacgaccgat ttacgatttt ccatacctgc ggtagcaggt ttacgaatca ggatattacc
     2581 tacctgatcg cgttcgacat ggaaaccttt ctcttttgcc caaccaacaa tgtattcagc
     2641 gagttgctct tcatgatagg acgggtgagg aatagaacag attttggcaa aaatatccca
     2701 cagcggctgt ggagataatt gagacagttc agacacgtta agtctccttg tcgatcaccc
     2761 gcaaaacagt attgcaggtc acagggttag cagaaaatgt tgtcaacaca agacaggctt
     2821 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     2881 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     2941 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     3001 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     3061 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctggtac
     3121 cgggtgcgtt actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca
     3181 gctacgatca cgacaaccag cgcgagctta aagtgctaaa acgcgcagaa ggcgatggcg
     3241 aaggcttcat cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg
     3301 aaatgtatcc aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg
     3361 ttgatgacta tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg
     3421 gcgtcgtatt cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg
     3481 gcgttgttct ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct
     3541 gcatccatga cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct
     3601 gaaacctcga cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc
     3661 cttgatggta aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg
     3721 cgcggcattg acccacgtga aatcctcgac gtccaggcgc gtattgtgat gagcgatgcc
     3781 gaacgtaccg acgatgattt atacgatacg gtgattggct accgtggtgg caactggatt
     3841 tatgagtggg ccatccaggc gatggtgtgg caacaaaaag cctgtgcgga agaagatccg
     3901 caactcagtg gtcgtcactg gctgcatgcg gctacgttgt acaacattgc tgcctatcct
     3961 catctgaaag gagatgacct ggccgagcaa gcgcaggctt tgtcaaaccg tgcctatgaa
     4021 gaagccgccc agcgtctgcc gggcacgatg cggcagatgg agtttaccgt acccggcggt
     4081 gcgcccatca ccggcttttt gcatatgccg aaaggcgatg gcccgttccc gacagtatta
     4141 atgtgtggtg gtctggatgc gatgcagacg gactattaca gcctgtatga acgttatttt
     4201 gcgccgcgcg gcattgcgat gctgactatt gatatgccgt cggtgggctt ttcatcaaaa
     4261 tggaagctca cccaggactc cagcctgttg catcagcacg ttttaaaggc tctgcctaac
     4321 gtaccgtggg tggatcacac tcgcgtcgcg gcctttggtt tccgtttcgg cgctaacgtt
     4381 gccgtgcgtc tggcatatct tgaatcgccg cgtctgaaag cggttgcctg tcttggtccg
     4441 gtagttcata ccctgttgag tgattttaag tgccagcaac aggtgccgga aatgtatctt
     4501 gacgttctgg cgagtcgttt ggggatgcat gatgcttccg acgaagcgtt acgcgtggag
     4561 ctgaatcgct attcattaaa agtgcaagga ttgctggggc gtcgctgccc aacgccaatg
     4621 ttatcaggct actggaagaa cgatccgttc agcccggaag aggactcacg cttaatcacc
     4681 tcatcatctg ctgacggtaa attattagag atcccattta acccggtgta tcggaatttt
     4741 gacaaaggtc ttcaggaaat caccggctgg atcgaaaaac gcttgtgtta aaaatttgct
     4801 aaaatttgcc aatttggtaa aacagttgca tcacaacagg agattgcaat gacgttaccg
     4861 agtggacacc cgaagagcag attgatcaaa aaatttaccg cactaggccc gtatattcgt
     4921 gaaggtaagt gcgaagataa tcgattcttt ttcgattgtc tggctgtatg cgtcaacgtg
     4981 aaaccggcac cggaagtgcg tgagttctgg ggctggtgga tggagcttga agcgcaggaa
     5041 tcccgtttta cctacagtta ccagtttggt ctgttcgata aagcaggcga ctggaagagt
     5101 gttccggtaa aagacactga agtggttgaa cgactggagc acaccctacg tgagtttcac
     5161 gagaagctgc gtgaactgct gacgacgctg aatctgaagc tggaaccggc ggatgatttt
     5221 cgtgatgaac cggtgaagtt aacggcgtga gtgaaatgtg ccggatgtat cacatccggc
     5281 aatatccatt aaaactgata ggtcatgcca accgcgacaa tatcatcatt attaatattc
     5341 aatttgttat cgctatccag ttggttgatt ttataatcaa caaacgctga catatttttg
     5401 ttgaaataat acgtagcacc gacgtcgata taattgacca gatcttcatc accgatacct
     5461 tcaatatctt tccctttcga taagacataa cccagcgatg gacgcagacc aaagtcaaac
     5521 tggtattgag cgaccgcttc aaagttctgt gtcttattgg caaagccgcc agttattggc
     5581 gtcattttgc gtgtttcaga atagaaagtt gccagataaa tattattggc atcgtatttc
     5641 agacctgttg cccatgcttc tgcacgcttg cctgtgccac ggctttgcag gttctgctcg
     5701 ttggtgcgat ctgagttggt ataggcccca ctaatggcga aatcgctgcc gccaaagtca
     5761 tatgtcaatg acgtgccgaa gccatcgccg ttttgctttt taacgtcgcg gttttcgttt
     5821 ttcccttgat attgcagggt taagttcagg ccatcgataa cgccgaagaa gtcggtgttc
     5881 cgatacgtcg ccagaccgct ggcgcgtttg gtcataaagt tgtcggtctg cgccgaggaa
     5941 tcgccaccaa attccgggaa catatcggtc caggcttcca cgtcatacaa cgcgcccagg
     6001 ttacgaccat aatcgaaaga acccaaatct ttatatttca acccggcaaa agcgagacgc
     6061 gttttctgct gtgcagtatc actctccgct ttatttccgg caaactccgc ttcccaccga
     6121 ccataaccag tcagttgatc gttaatttgt gtttcacctt taaaaccaaa acggatataa
     6181 ctctggtcgc catctttact ggcgttatca ctcatataat gcatagcttt aactttgcca
     6241 tagacatcca gtttattacc gtctttatta tatatttctg cagcctgtac agatgcagat
     6301 gccacaatgc ccatcaccac taatgccaga gtgctctttt tcattttcat tcctgatttt
     6361 aattaacgcg cgaatattca gcgggagagc cccgttgaaa acaggaaagt ttttaacctg
     6421 agattgttaa agatatatta cagattaata atattcttaa aatgtggtaa tttattaaat
     6481 ctgtaataaa agcgtaaaca actgccgcta ggcttgctga tcccgcgcaa caaaacgcca
     6541 tgctttgctc gcagatggtt ggcaaccgac gacagtcctg ctaaaacgtt cgtttgatat
     6601 catttttcct aaaattgaat ggcagagaat catgagtgac agccagacgc tggtggtaaa
     6661 actcggcacc agtgtgctaa caggcggatc gcgccgtctg aaccgtgccc atattgttga
     6721 acttgttcgc cagtgcgcgc agttacatgc cgccgggcat cggattgtta ttgtgacgtc
     6781 gggcgcgatc gccgccggac gtgagcacct gggttacccg gaactgccag cgactatcgc
     6841 ctcgaaacaa ctgctggcag cggtagggca gagtcgactg attcaactgt gggaacagct
     6901 gttttcgatt tatggcattc acgtcgggca aatgctgctg acccgtgctg atatggaaga
     6961 ccgtgaacgc ttcctgaacg cccgcgacac cctgcgtgcg ttgctcgata acaatatcgt
     7021 tccggtaatc aatgagaacg atgctgtcgc tacggcagag attaaagtcg gcgataacga
     7081 taacctttct gcgctggcgg cgattctggc gggtgccgat aaactgttgc tgctgaccga
     7141 tcaaaaaggt ttgtataccg ctgacccgcg cagcaatccg caggcagaac tgattaaaga
     7201 tgtttacggc attgatgacg cactgcgcgc gattgccggt gacagcgttt caggcctcgg
     7261 aactggcggc atgagtacca aattgcaggc cgctgacgtg gcttgccgtg cgggtatcga
     7321 caccattatt gccgcgggca gcaagccggg cgttattggt gatgtgatgg aaggcatttc
     7381 cgtcggtacg ctgttccatg cccaggcgac tccgcttgaa aaccgtaaac gctggatttt
     7441 cggtgcgccg ccggcgggtg aaatcacggt agatgaaggg gcaactgccg ccattctgga
     7501 acgcggcagc tccctgttgc cgaaaggcat taaaagcgtg actggcaatt tctcgcgtgg
     7561 tgaagtcatc cgcatttgca acctcgaagg ccgcgatatc gcccacggcg tcagtcgtta
     7621 caacagcgat gcattacgcc gtattgccgg acaccactcg caagaaattg atgcaatact
     7681 gggatatgaa tacggcccgg ttgccgttca ccgtgatgac atgattaccc gttaaggagc
     7741 tggccgatgc tggaacaaat gggcattgct gcgaagcaag cctcgtataa attagcgcaa
     7801 ctctccagcc gcgaaaaaaa tcgcgtgctg gaaaaaatcg ccgatgaact ggaagcacaa
     7861 agcgaaatca tcctcaacgc taacgcccag gatgttgctg acgcgcgagc caatggcctt
     7921 agcgaagcga tgcttgaccg tctggcactg acgcccgcac ggctgaaagg cattgccgac
     7981 gatgtacgtc aggtgtgcaa cctcgccgat ccggtggggc aggtaatcga tggcggcgta
     8041 ctggacagcg gcctgcgtct tgagcgtcgt cgcgtaccgc tgggggttat tggcgtgatt
     8101 tatgaagcgc gcccgaacgt gacggttgat gtcgcttcgc tgtgcctgaa aaccggtaat
     8161 gcggtgatcc tgcgcggtgg caaagaaacg tgtcgcacta acgctgcaac ggtggcggtg
     8221 attcaggacg ccctgaaatc ctgcggctta ccggcgggtg ccgtgcaggc gattgataat
     8281 cctgaccgtg cgctggtcag tgaaatgctg cgtatggata aatacatcga catgctgatc
     8341 ccgcgtggtg gcgctggttt gcataaactg tgccgtgaac agtcgacaat cccggtgatc
     8401 acaggtggta taggcgtatg ccatatttac gttgatgaaa gtgtagagat cgctgaagca
     8461 ttaaaagtga tcgtcaacgc gaaaactcag cgtccgagca catgtaatac ggttgaaacg
     8521 ttgctggtga ataaaaacat cgccgatagc ttcctgcccg cattaagcaa acaaatggcg
     8581 gaaagcggcg tgacattaca cgcagatgca gctgcactgg cgcagttgca ggcaggccct
     8641 gcgaaggtgg ttgctgttaa agccgaagag tatgacgatg agtttctgtc attagatttg
     8701 aacgtcaaaa tcgtcagcga tcttgacgat gccatcgccc atattcgtga acacggcaca
     8761 caacactccg atgcgatcct gacccgcgat atgcgcaacg cccagcgttt tgttaacgaa
     8821 gtggattcgt ccgctgttta cgttaacgcc tctacgcgtt ttaccgacgg cggccagttt
     8881 ggtctgggtg cggaagtggc ggtaagcaca caaaaactcc acgcgcgtgg cccaatgggg
     8941 ctggaagcac tgaccactta caagtggatc ggcattggtg attacaccat tcgtgcgtaa
     9001 ataaaaccgg gtgatgcaaa agtagccatt tgattcataa ggccattgac gcatcgcccg
     9061 gttagtttta accttgtcca ccgtgattca cgttcgtgaa catgtccttt cagggccgat
     9121 atagctcagt tggtagagca gcgcattcgt aatgcgaagg tcgtaggttc gactcctatt
     9181 atcggcacca tttaaatcaa taagttacac atcattagta ccttccttat tttttgactg
     9241 ggacaaattt gggaccgatg ggttcaggat cgagtctatt tgccgtgcgt gttcggtaag
     9301 gtgattaggt gcaaggtgag catatcgacg aaccatttcg atagactccc agcctcccat
     9361 ttcctgtaac actgacaacg ggactccggc ttgaaccagc caacttgccc aggtgtgtct
     9421 caagtcgtga aatctgaaat catcaatacc agcccgtctc agcgccgctt tccaggctgt
     9481 gtttgcgtca taccgcatct tccttactgt tggcgctttc gttccgtctg gtttggtaca
     9541 gctttccttg tacacaaata cccaacggtg atgattcccg atttgttttt tcaatacgcg
     9601 acatgcagta tcattcagcg caacgccaat tgcgcggttt gatttactct cttccgggtt
     9661 tatccatgcc acccggcgct gcatatctat ttgttgccat tcaaggttga tgatgttcga
     9721 gcgtcttaag cctgttgcca gtgcaaattc aacaacagac tttaatggct ccggacattc
     9781 atcaatcagc ctttgtgctt catggggctc cagccagcgg atccgtttat tctttggttg
     9841 aggcacttta ataattggtg ccttatccag cattttccat tcacgctctg cggctcttag
     9901 tagggccttt ataaatgaaa gatgcgtagc cttcgttgca acggacgctg gttttggcgt
     9961 gtattctgga acaggtttcc ctttttttct gcatgcttct gccctgagtt tccagttttc
    10021 ctcatgacgc cggttcgtca ttttctgcat tgctgaataa atttttgatt cagtaatgtc
    10081 tcttagttgc attcctgcga aatgttgaag ccagaatccg atccggcttt tgtcatcgtc
    10141 cagtgatttt ttatgtgctt tctcttcaag ccacctgaca cacgcttcct cgaacgttat
    10201 atcaggtatt tcaccaagtt tgctgacccg ccatgcttca gcctttagct tgtcatggag
    10261 ttctgtcgcc tgccttttgt cctttgttcc aagagactgt ttaaatcttt taccgttcgg
    10321 caatgtgaaa ctggcgtacc atatttcacc tctgcggaag agtgacattt tctttcctct
    10381 gttatgccat cacccgcgct cacctggaca gtatgcagcg gagactgaag agccgcaatg
    10441 caggcttgtc gtgttgtgag gtaaggagat ttattcttag tgggatcttt gcgtgttgcc
    10501 tgaagacgcc ctgtgcgtat ccagttaatg gcagtcggtc tggatatctt gagaaaatga
    10561 caggcctcat cgagtgtgag gctgtatggc tccattattt cacctcttgt tgtgacattg
    10621 ttgaaaaatg gataccagtt cgttgctgcc agacgatcca accgagagtc atatcccatg
    10681 ccatgtattc gttatcgccg ttttttgctc tccgacgatc tactaagtca ccgaaacgct
    10741 tttccatgaa taattcataa gcttcgcgtt catctggttc tacttccaga gataggagtg
    10801 cgatttcata agcacggcgc tcaatatcgt ctcgcacgtc aaggctgctg atacgctctt
    10861 taatttcttt aatcagttct ttgtcggtaa aagtggtcat tatgctccag cctccggtgc
    10921 ttttggcatt actgcccagt gagtgatatt gacgttttca aggtccccga cctgaaatgt
    10981 ccactgccat tctccggttt ctttttgtcc ccaggtgtac cagagagaac gccagccaat
    11041 tagccagcct tctccgttag catcgaataa caaaacactt tcatttgctg gtggcagttc
    11101 agttgacact ggtattactt tgttttcctg tgctgcacat ttagcttcaa gcgcatcgaa
    11161 tttacgcacc aggtattcaa catctgtttc attcactttc agatctcgcg gtacacatct
    11221 cccacgaaga aacccttcca tttcgaaaac attcatgcgc atttgcgtaa ctccgataac
    11281 tcgttaaaac gttccataaa catcccgtag gcatggcccg gtgcctgtgg aatcactttg
    11341 aacatctctg ttgccgggat accttccagt acaggccaga aagagccatc atcaagcccg
    11401 agatcgcggc gttcggttgc cagcataatg agatcggcat atttcactgg cgtgctcata
    11461 acaggaggta acccgtattt ctcacggatt acggcgtcta ttttttcttc catccgttta
    11521 tagtcaggaa gaagtcgttt cagtggtgcg gggatgtcct ggcaatatgc ttctgttgca
    11581 tcatgcatta aagcttcaaa agcaaattcc tgtggcacca gctggctgca aagcgccgca
    11641 tgttgggcga cactgtagaa gtgtgaaaga tgtcctgcaa agcgacagat atttgaaagg
    11701 gaaaccgcga tatcgttaat aacgatgtcg tctttattta tcctgtcata ataaaaatgc
    11761 ttcccggaaa aagttttaat aaatgacatt ttgttctcca cgtatatgcg ctgcaccgcg
    11821 ctgaattcgg gtaaaaggaa gccctcaccg tccggcgatt attgagtcaa ttacatttcc
    11881 ataaatgccc ccgtaggggc ggttagtttc tccacaaaac agagaagaac acctgcggtg
    11941 gcagccgccc ggatggattg ggttatgagc ccgtcgtccg gtgatgctct tctctgtttt
    12001 gtaaaaagga cggtaccagc cggaagcaag ggtacaagct ggtaccgcca agactacaca
    12061 cagcataaag ttgtggtgcc gggcgcctcc cggtgcctgg cgaaggttgc acaccaggcg
    12121 ggtgggtatc cacagaaggt cgactgtcag cctcaacctt tacccgcgtg cgctgagccg
    12181 cattcaccac aacgctaagg attctctctg gttgaaaata cttagctgtt atgtgcctgc
    12241 ttttagccac atcaggcgag gtggacctag ttattcccca acaacaagga tttggttatt
    12301 atgtaaccct ccccaacaac gataaggatt tatatggaac gatgtgtaga taagtatgag
    12361 gagaggcatg tgccgttaag acaaaagcct ccagttcctc caaaaccacc caaggaaaac
    12421 tgagactaat atgaccagag acgattataa attttatatt cactactctt atgtattgga
    12481 gaagatgaat tacacgttgt taactcgtat tgataaatta ataacgttga tgttaatcgt
    12541 tctcggtttt tcagttttcg ccccgtacag taatttattt attttcggtt tgtttgttgc
    12601 cgtactttcg gttcttcagt tggtctatca gttcggccag gaggctggga tgtctaaaga
    12661 acaagcgagg cattacaaaa gactgctgat tgagtctgat actctttctg aagatcagtt
    12721 gcaagctagg tatttaaaaa tccaagatac cgatagtaat ccatggaaat cacttgaacg
    12781 agcggctttc attcgggcct gcatttttgc tggtgttcca actaagtacg atctaacaac
    12841 caaagaaaaa tttattgctt ggtgtgcggg agatctacct ctagagaatg aactaacgga
    12901 tattaatgat gaccaacaag cataatccag ttcacggaca tgtacctccg aagccgaaac
    12961 caccgaaacc gaagaaatga gacataatag gattggataa tgagtcgacc aatagagcct
    13021 tttcatattc cacctcgtcc caggccacca gatccaggcc caaagtgagg gttactgatt
    13081 tttaaagagc gaagcgtccg gtagggcgct tttttgtttc taacgaatca tcctggactt
    13141 catatgctcc agggggctac ttcgtgggcg tcctgcctgt ttgttgtttc gcttgggtac
    13201 attatgtatc tcaaaggtac attgtcaagt ataaaaaaac ctgccgaagc aggttcataa
    13261 acattgatta ggcttttatt ttgtatcttc ttggttttcc tgagaaaatc actgtaccaa
    13321 ttatagagca attaccgttg atcttaatgt aaggctcagg ccagtttggg tttaacgctt
    13381 tgagataacg ctgtgttcca tcttctatca atcgcttgaa ggtggtttcg cctgtatcgt
    13441 gcatcaatgc aataacgtcg tcaccgtggc aggcagggac ttcgggatca acaaaaatca
    13501 tgtctccagg gcggtactca tcaatcattg aatcaccaat cacccgcaag atataagtca
    13561 tttcgccaca gggtacaggg cagggatacg tttctgctgt gctcaaatca acctcagaat
    13621 agccaacttc tttccatgct ccggcctgta cccatgatat gacagggact aacgttattt
    13681 gtttgttagt gattgaaaca tcaggttttt ttgtgatgtt cgttgtctgg tgttcttgat
    13741 caagccatcc gacaggcagg tcgaaacatt tttcgatgtg ccgtgccatg ctgtcaccga
    13801 tatttttagt agcgccatcc cccataaacc tgctggtttg ggttggctcg cgatcaatca
    13861 tggtggcaaa ggatgaattt ccgccaacac catctctcag ttttctggcg ttagaccgcc
    13921 ggatgtcatg gattgttttc ataacgaaat taaaacattt gtaccgataa ggtacaagta
    13981 tcttgaaggt tcatttcaat catgtaatat gtataccgga ggtacatatt gtatgaaagc
    14041 gtattgggac tctttaacca aagaacagca gggcgagttg gccggaaaag ttggctcaac
    14101 acctggctac ttacggctgg ttttcaatgg ttataaaaaa gccagttttg tgctggcgaa
    14161 aaaacttgag caatgcacgt caggtgcaat tacgaaatct gacttaagac cggatatcta
    14221 tccgaaagat taacagaaca ccttcaattt ttaaccacag aacgatgagg ctaaccgtgg
    14281 gtaagtatca ctggaaagta gaaaaacagc ctgagtggta cgtgaaagct gtcagaaaaa
    14341 ctatcgcagc gttgccgggt ggttacgctg aagcagctga ctggctggat gtaacagaga
    14401 acgcattatt taaccgcctt cgtgccgatg gcgatcagat tttcccgctg ggatgggcaa
    14461 tggttttaca gcgcgcggct ggcactcact acattgcgga tgctgtcgca cagtctgctg
    14521 gtggggtgtt cgtatcgctt cctgaaattg aggaagtaga gaacgccgat ataaaccagc
    14581 gcctgctgga agtcatcgaa cagatcggga attactcaaa gcagattcgt tcggcaatcg
    14641 aagatggggt cgtggagcca cacgagcaga cagcaattaa tgatgagttg tatctgtcaa
    14701 tttcgaagct ccaagagcat gcagcactgg tctacaaaat cttttgcgct ccagaaaaga
    14761 gtgacgcccg cgagtgtgca gctccgggcg tcgtggcgtt ttgtgtctgt ggagaaacta
    14821 acgcatgaac agtttaacgg caaataaccg tttgtcgcaa cagctggtgg tcagcgtcgc
    14881 tgaacacctg ttgttacggc atgaatgcag attaccaaat cacctggctg taagtaacca
    14941 cagagaactt tacctgactg tggggggcga gttgtgcagg aacttaaccg ctggtttcgt
    15001 gacggaagag gactttatgt tcatgttatt cgttgggagc cagaaacaca gcgcgttatc
    15061 tatcttcgca aagactaccc gcatgagtgc tttagtcctt tgtggaaatt caggcgtgat
    15121 tttattgagt gtgaaggacc accagcacat tgattctgcc attccgggac gttacactgt
    15181 tcaggcacct tataaagcgg gtgccgggat tggcgtcctg gaattgatca aggcgatata
    15241 tgacgcgcca gcgtcttttt tatcgtccgc atttgctcac atcaaagtta tggtgggctg
    15301 ggcgggggca tcgaaagatg cgccggtttc cttgatcacc ggttacgcca accccgttca
    15361 gttcaccacc agcgaaattg gcgtttccgg tggtggaagt atttcaccga tcaaggaggc
    15421 tgccatcatg gctactgtcc cagccctcac tcgtctgaat gatgaagact tacataaact
    15481 cagttatgta acaacagcac tacgtgctct gcgcaaggta actctttcag atccgcaggc
    15541 gcatcaagtt ctggtagaaa cccttcttaa cttgcaggct gaacgtattc gtctggcgga
    15601 taaggctaat tttcatattc accgtctcct gaatatcagc ggagggcatc gtcatgctta
    15661 atccgttgat cctcaatatt tgccgtttgc ttcagcgtaa aaaaacatca attcctacag
    15721 ttgggcagtg gtacaccaca cctgcagggc atgttctacg tgttagcctg gttgaccgtg
    15781 aatgccagaa ggtgatttgt gaaccgctgg gccgtaatta ccgcgtcagt atgccgctta
    15841 tagcctttcg ctccggaaaa aacatgaagc atctcggagg tgcagcatga gtatggagct
    15901 gatggttaaa gcgatgaaaa ttcgagtggg taatccattg cgaaaactgg ttctgatcaa
    15961 gctggctgat aatgccagcg atcagggtga gtgctggccc agctaccagc atattgctga
    16021 ccagtgcgag attagcaaac gttctgtgat gaatcatatt gcggcccttt gtgagtccgg
    16081 gctggtaaaa aaagtcaccc ggaaaggtga aaaaggtaac tcaagtaata tctatctcct
    16141 tcatctggat ggtgcaggag attcactagg gggtagtgca aataattcac tatctggtgc
    16201 agcaaattca ccaggtagtg caggagttgc accagggggt agtgcaggag attcacccag
    16261 aaccagtcac tcttttgaac cagtcaaaga accagtcaat gaaccaatag ctgttggtgc
    16321 atcagttgat gagtccgtgc gagttcgttc aaaccgaccg gaatattctc cggagtttga
    16381 gcaggcatgg ctggcatatc ccaaacgtgc tggtggcaat tcaaaatctg cagccttcaa
    16441 agcctggaaa gcccgtttga atgagggggt aaaccccgaa accatgctgg aaggtgtgaa
    16501 acgctatgcg ggctgggtat ctgcgatggg taacagcggc acacaatttg tgaaacaggc
    16561 tgtcacgttc tttggtccgg atcgtcattt cgaagaatcc tgggaagttc ctgcggtatc
    16621 tgcagccaga cgtgaggacc cgtacttcaa atccagttac gacaacgtgg actacagcca
    16681 gatcccggca ggattcaggg ggtgatcatg agtcttttga atgacgttca gaaattcatt
    16741 gaagcccatc cggggtgtac ttccggagac attgcggatg cttttgcagg ttactcacgg
    16801 cagcgcgtac tgcagtcagc aagcaagtta cgtcagagtg ggcgtgtggc tcaccgttgt
    16861 gaaggggata cacgcagaca tttcccgcgc ctgactgaga gagcgcagga gccggaacca
    16921 caaccagttc gtgaaaccag acctgtgcgc aatttctata tcggcactaa cgatccccgg
    16981 gtgattttgt gcctgacccg ccaggctgaa gaactggagt caagaggctt ataccgtcgt
    17041 gctgcaaccg tgtggatggc ggcattccgt gaaagccact cccagccaga acgaaacaat
    17101 tttctggcgc gtcgtgagcg gtgcttacgg aaaagcagca agcgcgctgc atcgggtgaa
    17161 gagtggtatc tgtcagggaa ttacgtgggg gcttaatgag taataaatat tgccaggcgc
    17221 tggtggaact gcggaacaaa ccagcccatg aactgaagga cgtgggcgat cagtggcgca
    17281 cgccggacaa cattttctgg ggaattaaca ccctgtttgg cccgtttgtt ctggatctgt
    17341 tcactgacgg tgataacgcc aaatgtgctg cgtattacac ggcggaagac aacgcgctgg
    17401 cgcatgactg gtcagaacgt cttgcggagc ttaaaggtgc tgcctttggt aatcccccat
    17461 acagccgcgc cagtcagcat gaggggcaat acatcaccgg catgcgttac atcatgaagc
    17521 atgccagtgc catgcgtgat aagggcgggc gctatgtttt cctgatcaaa gctgccacca
    17581 gcgaagtgtg gtggccggaa gatgcagatc atattgcttt tattcgcggg cgtattggtt
    17641 ttgaactgcc tgtctggttt atcccgaaag acgagaagca ggtaccgaca ggcgctttct
    17701 tcgctggtgc tattgctgtt ttcgacaaga cctggaaagg accggcaatc agctacatcg
    17761 ggcgcgatga acttgaggca tgtggtgagg cctttctggc gcaggttcgc cagcaggcag
    17821 aaaaactggt cagggagatg gcggcatgac gacgttaact caatgccagc agcaggtgct
    17881 ggatatgctg atttcttacc agaaagagcg tggctttccg ccaaccaatc aggaggtggc
    17941 aaccatgctg ggataccgtt cagtgaatgc agcggtagag catcttcgcg cactggagaa
    18001 aaaaggcgtc atcacgataa agcgtggcgt ggcccggggg ataacgcttc ataccgcggt
    18061 gaaggacgac gacagcgagg cggtcgggat tatccgcgca ctgcttgccg gtgaggaaaa
    18121 cgccaggctg cgtgcaaccc actggttaca tgagagaggc ctgaaagcat gaagctgatc
    18181 ctgccttttc cgcccagcgt gaacacgtac tggcgacacc ccaacaaagg ggcatttgct
    18241 ggtaagagcc tgataagcgc ggcggggcga aaatttcaga gcgcggcgtg cgcagcaata
    18301 gttgagcagt tacgtcgtct gccaaaacca acgtcggcac ctgcttcagt ggagatcgtg
    18361 ttgtttcctc cggataaccg gatccgcgat ctggacaact ataacaaggc gctgtttgac
    18421 gcgttgaccc acgcgggtgt gtgggaagac gacagacagg tgaaaagaat gctggtggag
    18481 tggggaccgg ttatcccgaa agggaaggtc gagatcacta tcagtaagta tgagaaaccg
    18541 gcgggtgcag ccgcctgatt aagaggagaa acgaagtatg aataatctga tggttattga
    18601 tggtattgaa gttcgtcgtg atgtttatgg gcgttacagc ctgaacgatc tgcatcgcgc
    18661 agcagtagca tctggtgcaa atgccagaac caaggagcca ggaaagtttc tttccagcca
    18721 acaaactgtt gaacttgttc atgaattgac caacacccag aatttgggtg ttgacccggt
    18781 gagtgtgatt catgggggaa atgaacgggg aacttatgtc tgcaaggaac tggtgtatgc
    18841 ctatgcaatg tggatcagcc cgtcattcca tctgaaggtg atccgtactt tcgacatggt
    18901 aaccagcaca ccggaaaaat tatccggaca ggctgctgac aagatgcagg ctggcgtgat
    18961 tctgctggac tttatgcgca gggagttaaa cctgtctaac tcttcagtgc ttggtgcctg
    19021 tcagaaactc caggaggctg ttggcttacc gaatctggca ccgcgctatg ccattgatgc
    19081 tccagctgat gcacacgatg gctcaagtcg cccgacactg tcactgagtg cactgctgaa
    19141 acagtatggt atccgcctga cggctaatca ggcatatcac cagatggtga agctggggat
    19201 cgtcgagcag cgcgaacgat acagccgtac cgcgattaac aacatcaaaa aattctggtc
    19261 gctgacagcg aaaggctgca tgttcggcaa gaacatcacc agtcccgcaa atccgcgcga
    19321 gacgcagccg catttcttcg aatcccgatt ccctgagctg ttaaagctgc tcgataccgt
    19381 tcattgaggt gaccgtgaga gcactactga cccctgaaat tgccccgcgt atggggatcg
    19441 tattgttcag gccaggttca gagctgatgc ccctgtttat gcaggggcgt gtcctgctgg
    19501 agcctgagcc ggagcgttat tcatctttcg ccagtggtgc cgttccggcg gcatcacaac
    19561 cgctggcgga tgatcctgcc gttcgggccg tgttccgcaa tgaggcagtg atccgtcgtg
    19621 ctggtggcgt ggaatgtctt gaaagctggt tacttcgtga aaaaggctgc cagtggcctc
    19681 attccaactg gcacagcgag aacatgacca caatgcgaca cgcgcctggc gcaatccgtc
    19741 tgtgctggca ctgcgataac caactgcgcg atcagttcac ggaacggctg gaatcaatgg
    19801 caacggataa ctgtgcccgc tgggtgttgt ctgttgtgcg tcgggatctc ggttttgatg
    19861 acagtcacgt tgtgacaatg ccggaactgt gctggtggct gattcgtaat gatctggcgg
    19921 atgccttacc ggaaagtgca gcccgtaagg cactgagatt accgaagcct gttgtgccgt
    19981 ctgttacccg ggaaagtgac cttgtgcctt cggttcctgc caccagcatc atccaggata
    20041 aggcgaaaaa ggtgctggcg ctgaaagtgg atccggagtc gccggagtct tttatgttac
    20101 gcccaaaacg tcgccgctgg gttaatgaaa agtacacacg ctgggttaag acacagccgt
    20161 gtgcatgttg tggaaagcct gctgatgatc cccaccacct gataggccac ggtcaggggg
    20221 gaatgggtac aaaagcgcat gatctctttg tgttgccttt gtgcagaaag catcacgacg
    20281 agctgcatgc ggataccgtg gcatttgaag agaagtatgg ttcccagctg gagctgatat
    20341 ttcgttttat cgatcgtgcg ctggcaatag gcgtactggc gtaagtggag aacgagcatg
    20401 aaccttgaag ccttaccgaa atattactcc ccgaaatctc caaaattgag cgatgacgca
    20461 ccggcgacag gctctggtgg tttaacaatt acggatgtaa tggctgcgca ggggatggtg
    20521 cagtcgaaag caccgcttgg gtttgcctta ttcctggcaa aagttggtgt tcaggatcct
    20581 cagtttgcga ttgaaggtct gctcaattac gcgatggcac tggataaccc gacattgaac
    20641 aaattgagtg aagaaacccg gttacagatc atcccttacc ttgtgaattt tgcctttgct
    20701 gattattcca ggtctgcggc aagtaaggct cgctgtgagc attgtgctgg tactggattt
    20761 cataatgtat tgcgcgaagt ggtgaaacac tccagaagcg gggaatctgt tatcaaggaa
    20821 gagtgggtga aggaactgtg tcagcattgt catggtaagg gagaagtcag cacagcgtgc
    20881 agagggtgta agggtaaagg tattgtcctg gatgaaaaaa gaacccggct tcatggcacg
    20941 cctgtttata aggtttgtgg gcgttgcaat ggaaaccggt ttagccgttt accaaccaca
    21001 ctggcgcggc ttcatgtcca gaagctggta ccagacctga cggattatca gtggtacaaa
    21061 ggatatgcag atgtcattga taaactggtt acaaagtgct ggcaggaaga agcatatgca
    21121 gagatacaat tgagaaaggt gacaagataa atggttttcg ccgaagatga cgacatgatg
    21181 cttgcgtttt tcaaaaaata tggataagat tttcacaacg atgggctttg tatgtctacc
    21241 gttgataaga tttaagaacc cgccactgag cgggtttttt tgtacctgta aacttggtgc
    21301 agtacagtaa acacgctggt ggtcgtgaat actgactttt tatcttgctg gctttttaga
    21361 caagagttac tggtatgtca tgttaaccag aagggaaaaa gacatgctaa aacagcaaga
    21421 tatgacagaa accgccgccg cagtccttca tttcttacct gctgacaagt gggtaacgcc
    21481 acgcatgatg acgagaacta ccggagtaag cgaagcccgg tgccagttaa tactgactca
    21541 gttagttctg gcggggctgg cgaaggataa cggcgggtac gggaataaat tcagacgctg
    21601 ccagtaatgg cggtttcctg ctgtgaaaat gggcggctgg tgggtgttgg tagcacctgc
    21661 cagccattcg ctcatgctta ctggtcacaa gcgaaccacg gcccactgct ttagcgcaaa
    21721 agcagagtga gcctaccaga gttacgctta ctgatccatg aaaaatactg taaaaataaa
    21781 cagtgttgat ttaatcaacg ctgattgcct gcattttatt cagtccctgc ctgatgattc
    21841 cattgacctg attgttaccg atccgccgta cttcaaggtg aaacccaacg gctgggacaa
    21901 tcagtggaaa ggggacgaag attaccttaa gtggctggac cactgtctgg cccagttctg
    21961 gcgggtgtta aaacctgccg gaagccttta cctgttctgt gggcatcgcc tggcatctga
    22021 tattgagatc atgatgcgtg aacgtttcaa cgtgcttaac catatcatct gggcgaagcc
    22081 gtccggacgt tggaatgggt gtaataaaga aagtctgcgc gcatattttc ctgccacaga
    22141 gcgcgttctg tttgctgaac attaccaggg gccatatcgc ggcaaaagtg acggctatgc
    22201 ggcaaaagaa agggaactca aacagcacat aatggcaccg ctgatatcgt atttcaggga
    22261 tgctcgtgcc gaactgggta taacggcaaa acaaattgcc gaagccacag gtaagaaaaa
    22321 tatggtttcc cactggtttg gtgccagtca gtggcagttg ccgaatgagg ctgactatcg
    22381 gaagttacag gcactgtttt cccgtatagc ggcagagaag tttcaggaac aacaactgga
    22441 acaaccacac caccagctgg tggcatctta tgattcactg aatcgcaaat attctgaatt
    22501 gctggatgag tttaaatctc tccggcgcta tttctccgta tcagtctccg tgccttatac
    22561 cgatgtctgg atgcataaac ccgttcagtt ctacccgggt aaacatccgt gtgagaaacc
    22621 ggcggatatg ctcaggcaaa taatcaatgc cagtagtcga cctggtgatc tggttgctga
    22681 tttttttatg ggatccggtt ccacaataaa agcagcaatg gcgctggggc gtcgggcctt
    22741 aggtgttgag cttgagtcag agcggtttaa ccagacagtg aaagagataa acgagctggt
    22801 ggggaaataa tctggtggcc acgtcaggtg gcctttttat ttccattaca cagcacccgc
    22861 atctgcgagg tggggttatg aaatccatgg ataagttaac aacgggtgtc gcctatggca
    22921 cctcagcagg tagtgccggg tactggtttt tacagttgct cgataaagtc acgccctcac
    22981 agtgggcggc aataggtgtg ctgggtagtc tggtatttgg cctgctgacg tacctgacaa
    23041 acctttattt caagattaaa gaagataagc gcaaggctgc gagaggtgga taatgcctcc
    23101 atcattacga aaagctgttg ctgctgctat tggtggcgga gcaattgcta tagcatcagt
    23161 gttaatcact ggcccaagtg gtgacgatgg cctggaaggt gtcagctaca taccatacaa
    23221 agatatcgtt ggcgtatgga ctgtatgtca cggacacacc ggaaaagaca tcatgctcgg
    23281 taaaacgtat accgaagcag aatgcaaagc cctcctgaat aaagaccttg ccacggttgc
    23341 cagacaaatt aacccgtaca tcaaagtcga tataccggaa acaacgcgcg gcgctcttta
    23401 ctcgttcgtc tacaacgtgg gtgctggcaa tttcagaaca tcgacgcttc ttcgcaaaat
    23461 aaaccagggc gatatcaaag gcgcatgtga ccagctacgt cgctggatat acgctggcgg
    23521 taagcaatgg aaaggcctga tgactcgtcg tgagattgag cgtgaagtct gtttgtgggg
    23581 gcagcaatga gcagagtaac cacgattatc tccgctctga ttatctgcat catcgtctgc
    23641 ctgttatggg ctgttaatca ttaccgtgat aacgccatcg cctacaaaga gcagcgcgac
    23701 aaagccgcat ccactatcgc tgatatgcag aagcgtcaac gtgatgtagc agaactcgac
    23761 gccagataca caaaggagct tgctgatgct aacgcgacta tcgaaagtct tcgtgctgat
    23821 gtttctgctg ggcgtaagtg gctgcgcgtc aaagctgtct gtccagacat gcataaaatc
    23881 accgccgcct ccggcgtgga tgatggcacc agccccagac ttactgacac cgctcaacgg
    23941 gattatttca cccttagaaa gcggattgaa accagtgata aaatgatccg aggcttgcag
    24001 caatacattc gcacgcagtg tgtaagatga gcaatctttg ctaattagcc atgaaataga
    24061 taaatatcag gccaacgatg attagtgcca ggcatcctaa ttggtcagaa tggctggcat
    24121 ttgatcgtct tgctcttctt gcagttgatt ggacagccct ctgtcctcga cttcctcttg
    24181 gcattcctct catttattct ctctcaacat tgatattgaa ctgacagatg ataattattt
    24241 catgaaagtg gtatctcgtt gatttgatta cgctacatag tcgccggatt ttcgcattta
    24301 tcggcatcgg gcggtgcaaa attggcataa tcgaaacgta gagtttttgg ctgacaacag
    24361 cattagcggt cacccggaga gtgttgtgaa catgttaatt acatgacgct ttagatgcat
    24421 cgacatttga tgatgcttgt cagacctgtc attttctttg cttcattttc aacattgatg
    24481 aagttggttg agcatatttt ttgataatct ccagttgtga atcctgtttt atcgatcttg
    24541 cgtttcaagg gattaatcgt tttgcaagat gctctatgga ttctggtaaa gcgttcgtca
    24601 gatttattac ctttacctct ggttcgcttc aatgcattga caacatatcc gtctggatta
    24661 tcgccaagcc aattacgata gtctgattca ctctcagtct gaaggtcact tctgaatacc
    24721 tttatggaca tgaagatact cctgtgcatt tatggtacga agaaatagca aaagtatttt
    24781 taccgtaaat tgcgaatcta caaaagcaaa acaatgcgta atatcagagt gaatattctg
    24841 cctttaatgt gggtccttct gatgacctga gctctcacgg ggcgggagcg tcgcggaaaa
    24901 aggctagttt ttgaaatttc attcgtcatc accactactt taatagattg atattacagt
    24961 gtttttattt ttatggtgtc gattttgatt gttttttgtt catcactaac accgtttgcc
    25021 taaagttgtt cgcaagatgc atgtttaaaa cattctggag cgggtatgga tcgagagttg
    25081 aaaaatctga cgctgaatat cagtcaactg gcggcactgt caggtgtaca tcgccagact
    25141 gctgcggcaa ggctgcaaaa tctacccgtt gcaggggggc atgaaagcaa cctcaagctt
    25201 tatcgggtgg ttgatattgt gtcggcattt ctggcattac caccgccggt tgcagaaggc
    25261 gaaatggacg cgcatgagcg caaagcctgg tatcagtctg aacgtgagcg tcttaagttc
    25321 gaacaggaaa cggcacaact cattccggcc agtgatgtca gacgggagtt tgccatctgg
    25381 gcaaaagcgg tcgtgcaggt gctggagaca ttaccggata ttctggaacg tgactgcggc
    25441 ctgcagcctg tcgctgtgag ccgtgttcag tccattattg atgatctgcg cgatcagata
    25501 gccctgcggg tgaccgaagc aggtgcggat gatgaggagg aattacagca ggaggagtaa
    25561 tgctgaatca ggaaaccgca aaggcagcac gaaccgattc aggttatatc cttcgcgcac
    25621 cgagacgaat gcgggttgct gatgccgttg ctcagtatat gcgagtgccc atgggggccg
    25681 ggaactcagt cccgtgggat ccgctggtgg caccgtatgt tcttgagccg atgaactgcc
    25741 tggcctcgcg tgaatacgac gcagtgatat ttgttggtcc ggcacgaacc ggcaagacta
    25801 tcggcctgat tgacggctgg gtgatttaca acgtgatttg cgatcctgcg gatatgctga
    25861 tcattcagat gacggaggaa aaagcccgcg aacactccaa aaaacgactt gccagaacgt
    25921 ttcgcgtcag cccggaagtg gtcagtcgcc tgagtccgaa caaaaatgac aacaacgttt
    25981 atgacagaac attccttgct ggcaactacc tgaaaatcgg ctggccgtca gtcaatatca
    26041 tgtcctcatc agattataaa tgcgtggcgc tgacggatta tgaccgtttt ccggaagata
    26101 ttgatggcga gggggatgcc ttctctcttg cctcaaaacg taccaccaca tttatgtcca
    26161 gtggtatgac gctggtggag agttcccccg gcagggatgt gaaggatgtg aaatggcgac
    26221 ggacttcacc gcatgaggct ccaccaacca cggggatact gtcgctctat aaccgtggcg
    26281 atcgccgtcg ctggtactgg ccctgtccac actgtggtga gtattttcag ccctgcggcg
    26341 atgtggttgc tggtttccgt gatattgccg atcccgtgct ggcaagtgag gcggcttata
    26401 ttcagtgtcc ttcctgttca ggacggatta tgcctgaaca aaaacgtgag ctgaacggac
    26461 gtggggtctg gttgcgggat ggtgaatcca tcaatgcgga tggcagtcgt tatggtgatc
    26521 cccgacgctc acgtattgcg tcattctgga tgtagggtac atttcttacc tgtttttatg
    26581 ttctggtgtc gttttgtagt cttttcaatg agttgtgatt ttttgtgttt cctctcttta
    26641 cttgataatg agttagttta tcgcttgtta tcgacttgaa aggactacat gacggactaa
    26701 aaaatgaggg cgatagatgc cggtaaagcc attaaccgtg actgaagtta agggaatgaa
    26761 accacgtgaa aaggactatg ccgtttatga tgggttcggt ttattgctga atgtgagtaa
    26821 agctggtggg aaagtgtggc gtttccgtta tagccatccg ataacgaaga aacggcagac
    26881 atacacgata gggcgttttc ctgaattctc actcgcggaa gcacgggaaa tacgtgatga
    26941 acttcggcga atgattgcac gtggagttga tccagtgaca gagaagaaaa atcgtaaaat
    27001 tgagatgtca ctaaaaaatc tacaaacatt tgaagctgtt gctaatgcat ggatcgcttt
    27061 taaaaaggga tcggaattgc ggaaacctac gctgtataat atcgaatatg aagtatacaa
    27121 atatcttgtt cctttctttg gtaagtacag catagagaaa attacagcgc cagtagctat
    27181 taatgctctg gatgccgtat ccgataagaa tgcgttgcaa aaaaaattaa tatcaagatt
    27241 aaatgaaatt atgaattacg ctgtaaattg tggagcattg aaaacgaatc cattacttaa
    27301 gataaaaact gcattcacag gaaagaaaaa taaatcatta gctgcactac ctgttgaaag
    27361 attgcctgaa tttctgagtt ggtgggatag tgtgcctcat aagtatcaaa tagctcacaa
    27421 tgcactttta ttccagatat tgacaatggt caggccaggt gaggcgatta aagcagagtg
    27481 gtccgagatt gattttgatt ctggcttgtg gattatcccc gcgcataaaa tgaaatgcca
    27541 tcgtgaacat gttgtgcctc tctcatcaca ggctattaga atcctaagaa caatgcagaa
    27601 aataaaaaga gggcgttata tatttttttc ctccagaaca aaagatgctc ctatgggaaa
    27661 gaatactatc aataccccaa ttgctgccag caagttcaaa gggattgtaa cgttacacgg
    27721 ttttcgttcg atgtggagta cgcttttaaa tgaggagggt tttaaccctg atgttattga
    27781 ggctgcatta gcacataaaa gtggtgataa aataagagat atttataata gaactactta
    27841 tttagaacaa cgcaaaatta tgatgcaatg ggtaggagat ttttttgatg aggcgagaaa
    27901 aggggtaatt aatagatccg gtggtaagaa aggtttgaga atagtaaatg gttgaggagt
    27961 tcagcaaatg aataccaatg aagatatttt atttactaaa gacgtaatga aaattttgcg
    28021 ctatggggca atgagcgcat tcatcaattt ctggaaagat gaaaataatg gttttcctca
    28081 accgttcaga attgggcggc ggcatacctg gcaccgtaga gatgtagagg catggttaga
    28141 taaacaacga gaacaggcca atccccacta ataatatctt tcataccccg cacgcaatgc
    28201 ggggtttttt gtatgtgagg taaaaaacga tgaataaaaa tattgccgtg acgggcaagg
    28261 ggtacgctcg ttcagtaaaa aaattctgcg atattcgtga tcttgtcgtt ctgcgctttg
    28321 atggcgtgga tgttcgtgtg gtgtatctga acggcgatcc gtggtttgtt gcaaaggatg
    28381 tttgtgaagc tctggaaata agcaactcac gcgatgctct aaaagctctg gatgtggatg
    28441 agaagaatac cgtcgcttta agctacggta ttcgcggaaa tccaaatcat agcgtcgttt
    28501 ctgaatctgg tttttacaag ctgatcgcca gaagtcgcaa agctgttact cctggcacgt
    28561 tcgcccatcg tttcagtaac tgggtattca ggaatgtgat accaggtatc agaaaaacgg
    28621 gggcttatgg tatcccgtgg ggtgcattac aggatttttc ccgccgtaaa gagcaatatc
    28681 aaataagtgc cagcgagaag gggagggagc tacaggcatg taagcgcaaa aagcgtgagc
    28741 tggaggaaga agaaaaaagg ctgatacgtg aatatcagcc tgagttttac tttggtgagc
    28801 gtattcagta accacgcgca ttgctgatta tacgatacat cgtgttaacc gggaagctcc
    28861 ccaccagcaa ggcaaaatct tctgctaaaa aatgacatat gagcagccgt cctgaaagca
    28921 tgaagttttg caggaataga tgctgcagtt ctttatttta tcaattggac tttagataaa
    28981 agtaaaggcg accgaggtgg tcgccagttt tatttttata tttttcccta ttgcatcaac
    29041 aatgaatgtc ttatccacat tacgagggat ggaataattt ttatgtatcc gccatatgcc
    29101 catataatgg tgcaaaaaac agttagtcca accccgagta cttcagcttt tatttcctct
    29161 ctgacctcaa ctcgagcttt agctaagttt tcttctcgta tcttttgtga ttcttccgca
    29221 gacaatggcg ggccagttac gccactgcgt gaattaattt cagcttcgcg attagataat
    29281 ttttttaaag ttgtgaactt gattgtatac aacaatccaa aaactgaaat aattgcacct
    29341 gagctggcga atagattcat atcattaaga tattggccga acaaaaagct tgctccacaa
    29401 aaaacacata atatggcaat ggatgttttt gcaccgagta tttttagtaa tattgttcta
    29461 aatctcttca ttgttaatat atcctatcat agaagtagta attcacccct taggttcgat
    29521 accgcgccgc tttagttcgg agcgccctaa ctctttaagc cagttagcaa ggcttacacc
    29581 atcattatta gcctcgctat caaactgttc tttcagttct ggagctatac gcatatggaa
    29641 agggggagcc tttcctgtcc ctttgggttt tttatcgcgt ttaattattg ttgacatgtg
    29701 tacacctatg gcgttatgtt ttcattcgat aggtgtacac attaacacgt gtgcatttaa
    29761 taacgcaacg ccccgcagtg ctggaacaca tgcagggcgt ctaaccacca acgatagcaa
    29821 cagtatcgag gtagctatgt taaatcatac cacacacccg caagggcggg actcgcacga
    29881 cctgaataaa tacatctggc gttttatcgc cctgagcacc gcacaaccgc gcgtgattca
    29941 catcgtggcc agcagcgaac aggaagcacg ccagcaatcc ccggctggct gcgtgatggt
    30001 attcgccgcc cgtattcgtc agggggtgtg ccatgcctga tatgtcaaat taccagtacc
    30061 tgattaatcc gcattttaac tgtgagcatg atattgctaa aaaggtttat tccgctgcgg
    30121 atggagctac tgacaatata tcaatggctg ttgcgtcaat tggtagcctg atgtggcatg
    30181 cgtcagaaaa tgaggactat gacgaaaagg ccatgcgcat tgatatgggt aatatcggtt
    30241 tgttactggc aatgcttgga cattttgata tttcgttacg gtgcaccatt gaaaatgcca
    30301 cagatgcatt aaatgctata aagaaagcga atactgattc aaatcgggga taaataatca
    30361 tgagaacgta tttatctggc ttgactgcca gcggttatgc acacccccaa attatccccg
    30421 gcgctattta tctggataag aacggtaaca gagtaacggt aaaagaactg atgtttgacc
    30481 gtgtgtattt tatccgtgat ggctattcat ttcttagttc gctgaacgtg gagatcttta
    30541 tttgcagatt ccggcgggaa atcccgactt ccagaaataa ccatgtgtca cgtgtggatg
    30601 tggataaaaa actacaggaa ctgaaaaaca tgattgccgc gtggagagag cagaaatgaa
    30661 aaaagcgcca aatttaaaac accagccgcg tgacaaaatg acggaagtca tcatttttgc
    30721 gggtagtgat gcgtgggcac atgcgaagca gtggcaggaa caggacgggc gactggctgg
    30781 cgataacgtg ccacctgtct ggcttggaga gcaacaactt gccgaactgg acaacctgca
    30841 aatcgtaccg gacggacgct atcgcgtgcg tctctaccag gcggggttat tgcgtccggg
    30901 gcttgttaat accatcgggc agaaactggc agcggcaggt gtcagggatg ctgattatta
    30961 ccctgaagga atgcacagcc agaaacggga gaactggcgc gaatatctgg aacgtgagcg
    31021 gggagagctg acggaaaaga aaaaggtagt tgaactgcct gtaaagaaaa aagagcgggt
    31081 aaaagacgat aacgcttcat cactggcgct taaccagatg ggagcaagtc aacgcggcga
    31141 agttctcctg gcacattatg gcggtgaact ggcgattcat gccgactctg acactgttca
    31201 ccattacaac ggcgttgtat gggagccagt acaggataaa gaattacagc gagctatggc
    31261 gcagattttc attgatgcgg agatcagcta ttcgcagaac gccattaaat cggcggtcga
    31321 taccatgaag ttaagtttgc ctgtaatggg gaatacagcc cgtaacctga ttggattcag
    31381 taacggggta tttgatacca gaacaggtaa ttttcggcag cataacaaaa acgactggtt
    31441 gttaattgcc agtgaattac ctttcagccc accagcagag ggggaaacgc tggcaacaca
    31501 tgcgccgaat ttctggaagt ggttacgccg ttcggtggct gagaatgacc gcaaggcgga
    31561 tcgcgtactg gctgcattat tcatggtgct ggcgaaccgg tacgactggc agttattcat
    31621 tgaggtaaca ggtccagggg gaagtggtaa aagcgtgatg gcggagattt gcaccatgct
    31681 ggcgggtaag gctaatacag tatcagcgag catgaaggcg ctggaagatg caagggaacg
    31741 cgcgttagtg gttggctttt cgctgattat catgccggat atgacccgct acgctggtga
    31801 tggggcggga attaaggcta ttacaggcgg tgacaaggtg gcaatcgacc caaaacataa
    31861 agccccatat tcaacgcgta tcccggcagt agtgctggcg gttaacaata acgctatgtc
    31921 attcagtgac cgcagtgggg ggatctcgcg ccgtcgggtg atattcaatt tttcggaagt
    31981 tgtaccggag aacgaacgcg attcaatgct ggcggaaaaa atagaaggtg agctggcggt
    32041 agtgattcgt catctgctta cacggtttgc tgaccaggac gaagccagac gccttttata
    32101 tgagcagcag aaatcagaag aagcactcgc gataaagcga gagggggatt cgctggtgga
    32161 cttctgcggc tatctcatgg cgtcggtaat gtgtgatggc ctgttagtgg gtaatgctga
    32221 aattgtgcca ttcagcccgc gcaggtatct ctatcatgcc tatctggctt atatgaaggc
    32281 acatgggttt ggtaaacctg taacactgac gcgcttcggt aaagatatgc cgggggcaat
    32341 ggcggaatat ggcagggagt atatgaaacg gaaaacgaag cacggtttgc gttcaaacgt
    32401 gacactgacg gaggaatcag aagactggat gc
//
LOCUS       DAEEKQ010000026        32270 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.026, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000026 DAEEKQ010000000
VERSION     DAEEKQ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 32270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32270
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.026"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            28..591
                     /locus_tag="KLK57_001258"
     CDS             28..591
                     /locus_tag="KLK57_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0891681.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTCAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            673..3315
                     /gene="yfcU"
                     /locus_tag="KLK57_001259"
     CDS             673..3315
                     /gene="yfcU"
                     /locus_tag="KLK57_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HBE0891682.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNIGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3335..4086
                     /locus_tag="KLK57_001260"
                     /pseudo
     CDS             3335..4086
                     /locus_tag="KLK57_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            4061..4609
                     /locus_tag="KLK57_001261"
     CDS             4061..4609
                     /locus_tag="KLK57_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0891683.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4606..5076
                     /locus_tag="KLK57_001262"
     CDS             4606..5076
                     /locus_tag="KLK57_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0891684.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTLSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLMVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5073..5597
                     /locus_tag="KLK57_001263"
     CDS             5073..5597
                     /locus_tag="KLK57_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0891685.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            5584..6456
                     /locus_tag="KLK57_001264"
     CDS             5584..6456
                     /locus_tag="KLK57_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HBE0891686.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVTYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVTSYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6578..7129)
                     /gene="smrB"
                     /locus_tag="KLK57_001265"
     CDS             complement(6578..7129)
                     /gene="smrB"
                     /locus_tag="KLK57_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HBE0891687.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIPEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7295..8227
                     /gene="prmB"
                     /locus_tag="KLK57_001266"
     CDS             7295..8227
                     /gene="prmB"
                     /locus_tag="KLK57_001266"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HBE0891688.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREYFAIYKD"
     gene            8262..9347
                     /gene="aroC"
                     /locus_tag="KLK57_001267"
     CDS             8262..9347
                     /gene="aroC"
                     /locus_tag="KLK57_001267"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HBE0891689.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9351..10175
                     /gene="mepA"
                     /locus_tag="KLK57_001268"
     CDS             9351..10175
                     /gene="mepA"
                     /locus_tag="KLK57_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HBE0891690.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFAPLKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10175..10984
                     /locus_tag="KLK57_001269"
     CDS             10175..10984
                     /locus_tag="KLK57_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HBE0891691.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            10984..11532
                     /gene="epmC"
                     /locus_tag="KLK57_001270"
     CDS             10984..11532
                     /gene="epmC"
                     /locus_tag="KLK57_001270"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HBE0891692.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     ALDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11566..11844
                     /locus_tag="KLK57_001271"
     CDS             11566..11844
                     /locus_tag="KLK57_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HBE0891693.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
     gene            complement(11965..13971)
                     /gene="mnmC"
                     /locus_tag="KLK57_001272"
     CDS             complement(11965..13971)
                     /gene="mnmC"
                     /locus_tag="KLK57_001272"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HBE0891694.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLNEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLTALNPNRLWVRKLLKGKAVKAG"
     gene            14130..15350
                     /gene="fabB"
                     /locus_tag="KLK57_001273"
     CDS             14130..15350
                     /gene="fabB"
                     /locus_tag="KLK57_001273"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HBE0891695.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            15625..16803
                     /locus_tag="KLK57_001274"
     CDS             15625..16803
                     /locus_tag="KLK57_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0891696.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGFI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTEFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(16800..17795)
                     /gene="flk"
                     /locus_tag="KLK57_001275"
     CDS             complement(16800..17795)
                     /gene="flk"
                     /locus_tag="KLK57_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HBE0891697.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVNDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            17894..19030
                     /gene="pdxB"
                     /locus_tag="KLK57_001276"
     CDS             17894..19030
                     /gene="pdxB"
                     /locus_tag="KLK57_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HBE0891698.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            19096..20109
                     /locus_tag="KLK57_001277"
     CDS             19096..20109
                     /locus_tag="KLK57_001277"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBE0891699.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            20109..20921
                     /gene="truA"
                     /locus_tag="KLK57_001278"
     CDS             20109..20921
                     /gene="truA"
                     /locus_tag="KLK57_001278"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HBE0891700.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPERWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            21004..21663
                     /locus_tag="KLK57_001279"
     CDS             21004..21663
                     /locus_tag="KLK57_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0891701.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGDKLFS
                     NPDSKIFRRSYLDKTHQFYERHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLIFTYAGYFFGTLPFIQSNLKLMIVGIIFVSILPGVFEFIRHKRAAARAA
                     K"
     gene            21819..22733
                     /gene="accD"
                     /locus_tag="KLK57_001280"
     CDS             21819..22733
                     /gene="accD"
                     /locus_tag="KLK57_001280"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HBE0891702.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVHRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            22803..24071
                     /gene="folC"
                     /locus_tag="KLK57_001281"
     CDS             22803..24071
                     /gene="folC"
                     /locus_tag="KLK57_001281"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HBE0891703.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSAKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            24061..24723
                     /gene="dedD"
                     /locus_tag="KLK57_001282"
     CDS             24061..24723
                     /gene="dedD"
                     /locus_tag="KLK57_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HBE0891704.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            24982..25470
                     /gene="cvpA"
                     /locus_tag="KLK57_001283"
     CDS             24982..25470
                     /gene="cvpA"
                     /locus_tag="KLK57_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HBE0891705.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            25507..27024
                     /gene="purF"
                     /locus_tag="KLK57_001284"
     CDS             25507..27024
                     /gene="purF"
                     /locus_tag="KLK57_001284"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HBE0891706.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            27119..27688
                     /gene="ubiX"
                     /locus_tag="KLK57_001285"
     CDS             27119..27688
                     /gene="ubiX"
                     /locus_tag="KLK57_001285"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HBE0891707.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTHDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            27954..28736
                     /gene="argT"
                     /locus_tag="KLK57_001286"
     CDS             27954..28736
                     /gene="argT"
                     /locus_tag="KLK57_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HBE0891708.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKAFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            28957..29739
                     /gene="hisJ"
                     /locus_tag="KLK57_001287"
     CDS             28957..29739
                     /gene="hisJ"
                     /locus_tag="KLK57_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HBE0891709.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            29829..30515
                     /gene="hisQ"
                     /locus_tag="KLK57_001288"
     CDS             29829..30515
                     /gene="hisQ"
                     /locus_tag="KLK57_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HBE0891710.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            30512..31228
                     /locus_tag="KLK57_001289"
     CDS             30512..31228
                     /locus_tag="KLK57_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0891711.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            31236..32009
                     /gene="hisP"
                     /locus_tag="KLK57_001290"
     CDS             31236..32009
                     /gene="hisP"
                     /locus_tag="KLK57_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HBE0891712.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
BASE COUNT         7563 a         7992 c         8729 g         7986 t
ORIGIN      
        1 taattattta tagtttaagg acaaataatg agtaagtttg ttaaaacagc tattgctgcg
       61 gcaatggtga tgggcgtgtt cacctctacg gcaacaatcg ctgcgggtaa caacggtact
      121 gcacgttttt acggcaccat tgaagactca gtgtgttcta tcgttccgga cgatcacaaa
      181 ctggaagttg atatgggtga tatcggtgcc gaaaaactga aaaataacgg caccaccacg
      241 ccgaaaagtt tccagattcg tctgcaagat tgcgtatttg atactcagga aacaatgacc
      301 actaccttta ctggtaccgt ttcttctgca aatagcggca attattacac cattttcaat
      361 accgatacct gtgcggcatt taacaatgtt agcctggcga tcggtgactc tctgggtacc
      421 tcttacaaaa gcggcatggg tattgaccag aaaatagtga aagacacttc taccaataaa
      481 ggcaaagcga agcagacact gaactttaaa gcctggctgg tcggcgcagc tgatgcgcca
      541 gatctgggta atttcgaagc aaataccacc ttccagatta cttacctgta aggcataccc
      601 ccatagcgac actgctatgg gggatttaaa tggattttga ccgaacaaga ataaaaacgg
      661 atagaaacgt gtatgcctga ccattctctt tttcgattac ggattcttcc gtggtgcatt
      721 gcgctggcaa tgtcagggag ttatagcagt gtctgggctg aagacgacat tcagtttgat
      781 tcccgttttc tggaattaaa aggcgacaca aaaattgatc tgaagcgttt ttccagccag
      841 ggatatgttg agcccggaaa atacaattta caggttcaac taaataaaca gccattggcg
      901 gaagagtacg atatttactg gtatgctggt gaagatgacg cgagcaaaag ctatgcttgt
      961 ctgacaccgg aactggtagc gcagtttggt ttaaaagaag acgtggcgaa aaatctgcaa
     1021 tggagccacg atgctaaatg cctgaaatcc ggtcaactgg aaggcatgga aattaaggct
     1081 gatttaagcc agtccgcatt agtcatttca ctgccacagg cttacctcga atatacttat
     1141 cccgactggg atccgccttc acgttgggat gacggcatct ccgggatcgt cgcggactac
     1201 agcatcaacg cacaaacccg gcacgaagaa aatggcggtg atgatagtaa cgagatcagc
     1261 ggcaacggga cggtcggggt taacctgggg ccgtggcgta tgcgtgctga ctggcagact
     1321 aactatcaac atactcgcag taatgatgac gatgaattca gcggcgatga aactcaaaaa
     1381 aaatgggagt ggagtcgcta ctatgcctgg cgggcgttac catcattaaa agccaaactg
     1441 gcgctgggcg aggattacct cagatccgat atttttgatg gttttaacta tgttggtggc
     1501 agtgtcagta ctgacgatca aatgttgcct cccaatctgc gcggctacgc gccagacatt
     1561 tccggcgtgg cacacaccac agcaaaagtg accgtcagcc agatggggcg tgtgatttac
     1621 gaaacgcagg ttccggctgg accgtttcgt attcaggatc ttggtgattc tgtctccggt
     1681 acgttgcata ttcgcattga agaacagaac ggccaggtgc aggaatatga catcagcacc
     1741 gcctcgatgc catacctcac tcgtccaggt caggttcgct ataagatcat gatgggccgt
     1801 ccgcaagagt ggggacacca tgtcgagggt gaattttttt ctggtgctga agcttcctgg
     1861 gggatcgcta acggctggtc gttatatggc ggcgcactgg gagatgaaaa ctatcagtct
     1921 gcggcgcttg gcgtcggtcg cgatttgtct acattcggcg cggtcgcgtt tgatgttact
     1981 cactcgcaca ccaaactgga taaagacacc gcttatggca aaggttcgct ggacggtaac
     2041 tccttccgtg tgagttattc caaagacttt gaccagctca acagccgcgt tacttttgct
     2101 ggatatcgct tctcggaaga gaactttatg accatgagcg agtatctgga tgccagtgac
     2161 agcggaatgg tacgcacggg caacgacaaa gagatgtaca ctgccactta taaccagaac
     2221 ttccgcgatg cgggtgtttc ggtttatctc aactataccc gccataccta ctgggatcgc
     2281 gaggagcaga caaactacaa catcatgctc tcgcactatt tcaatatagg cagtattcgt
     2341 aatgtcagca tctcgatgac tggctaccgt tacgagtatg acaaccaggc cgacaaaggc
     2401 atgtacattt cgctcagtat gccgtggggt gacaacagta ccgttagcta taacggtaac
     2461 tatggcagtg ggacggacag cagtcaggtc ggttatttca gccgtgtcga tgacgcgact
     2521 cactatcagt tgaacgtcgg caccagtgac aaacacacca gcgttgacgg ctattacagc
     2581 catgatggtt cgctggcgca ggttgacctc agtgcgaact accatgaagg gcaatacacc
     2641 tctgcgggct tgtcgttaca gggcggcgca acgcttactg cccacggtgg cgcacttcac
     2701 cgtacccaga atatgggcgg gacacgcttg ttgattgatg ccgatggcgt tgccgatgtt
     2761 ccggtagaag gtaacggtgc tgctgtttat accaatatgt ttggtaaagc cgtcgtttct
     2821 gacgtcaata actattaccg caatcaggcg tatatcgacc tcaacaaact gccggaaaac
     2881 gccgaagcaa cccagtcggt ggtacaagcc acgctaactg aaggggcgat tggctaccgc
     2941 aaatttgccg tcatcagtgg tcaaaaagcg atggcggtgc tgcgtttaca agacggcagc
     3001 catcctccgt ttggcgcaga agtaaaaaat gataacgagc agacagtggg ccttgtcgat
     3061 gatgacggca atgtttatct ggctggggtg aaacctggcg aacacatgag tgtgttctgg
     3121 agtggtgttg cgcattgcga tatcaacctg ccggacccgc tgcctgccga tctgtttaac
     3181 ggcttgttac tgccatgcca gcataaaggc aacgtcgcgc cggttgttcc tgatgacata
     3241 aagcctgtca ttcaggagca gacgcaacag gtgacaccca ccaatccccc tgtttctgtt
     3301 tcagctaacc aataacgtga ttaaggaatg atccatgtct gatttacttt gctctgccaa
     3361 attgggtgct acgacgctag cgttactgct tagcgctgcc agtttgtcgc ccaggcttct
     3421 gtaacgccag atcgtacccg tttgatattt aatgaaagcg acaaatcaat cagcgtgacg
     3481 ttgcgcaata acgatccgaa actgccttat ctggcccaaa gctggatcga agatgaaaag
     3541 ggtaacaaga tcagttcacc gttaactgtt ctgccaccgg tgcaacgcat cgattcaatg
     3601 atgaacgggc aggtaaaagt gcagggaatg ccggatatca acaaattgcc tgccgatcgt
     3661 gaaagcctgt tctacttcaa cgtgcgtgag atcccgccaa aatcaaacaa agccaacacg
     3721 ctgcaaatag cactacaaac gcgtattaag ttgttctggc gaccgaaggc gctggagaac
     3781 gtcagcatga aaaacccctg gcaatataag gttactttaa cccgcaacgg tcaggaattt
     3841 accgtcaata acccgacgcc gtattacgtc attttcagta atgccagcac acagaaaaat
     3901 ggcaatcctg cagccggttt ctcgccaata gtgatgtcgc cgaaaacctc tgcaccgctg
     3961 aatgtgaaga tgggtagcgt gccggtactg acctatgtca acgactatgg cgctcggatg
     4021 ccgctctttt tcacttgcaa cggtaacagt tgccaggtcg atgaagagca gagccgcaaa
     4081 ggctgaggcc gggagagcca gtatgaaagg atcttgttct gtctgggcgg taactgggtt
     4141 actgatatgt tgtgttggta gcggctttgc agttgcagcg agtaagtcag taagtatgaa
     4201 tttacgggtg cttgtcgatg cccctcctcc ttgtacggta aatggtgcgg cagttgagtt
     4261 tggtaatgta ttcattaata agatcaatgg ggttgattat aaacgtccca ttgactactc
     4321 gcttgtttgt aacaatctgg caatggatga cctgcgtcta cagatgcagg cgacaacggt
     4381 cgtgattaac ggtgaaacag ttatcagcac gggtattcct gggtttggta ttcgggttca
     4441 gaaaagtagt gatcacacaa tactcgactt aacctctggc tcatggttgc catttaattt
     4501 ttcctcggga gttcccgttc tggaagctgt gccggtgaaa cagagtggta caacgttagc
     4561 ggcagctgaa tttaacgcca gtgccacaat tgtggtggat taccaatgaa acggatctcg
     4621 cttattttgc tgtggggctt ttgctcgatg gcactctcaa acgtctcttt tcacggttat
     4681 ttagtgcagc cgcctaactg cacgattagc aacgctcaaa ccattgaaat aacttttcag
     4741 gatgtgctta ttgatgacat taatggcagc aattatgagc aaaccgtgcc gtattccatt
     4801 acctgcgata cggcggttcg cgacccatta atggaaatga ccttgagctg gagtggcaca
     4861 ctaagcgatt ttgataatgc tgcagtaagc agcaatatca ctggtttagg cattcagctc
     4921 aaacaggcag ggcagagttt tacgatcaat acgccactta tggtgaatga aaccgattta
     4981 ccggtgttaa cggctgtgcc agtaaaaaag agtggtgtcg ttctgccaga ggcggacttt
     5041 gaggcctggg ccacgttaca ggtagattac caatgaagaa aaagcgtacg ttgtttttta
     5101 ttagtagcct gatgttgctg ggcagtggaa ccacaatagc aggagataat ttgcacttta
     5161 ccggcaattt aatcagtaaa tcgtgtacgc cagtgattaa tggtagccag ttggcagaag
     5221 tacattttcc tgccattgcc gccagcgatt taatgaacct tgggcagtca gagcgagtgc
     5281 cattggtgtt tcaattgaaa gattgtcata gttctacatt gtttaatgtc aaagtaacgt
     5341 tgacaggaac agaagatagt gctctacccg gctttctggc ctttgactcc tcgtcatcag
     5401 catccggcgc tgggattggt attgaaactg cggcaggcac atcggtgcca atcaacaata
     5461 caacaggcgt cacgctcccg ctgaatcagg gaaataacag cctaaatttt aatacctggt
     5521 tacaggcaaa gagcgggcga gatgtaacgt ccggagactt tagtgccaca gtgacggcaa
     5581 cctttgagta tttctgaaat gaaaaaatgg accataattt tcgcgagcct aatgctgctc
     5641 gttctgagtg ttgtcggcgc atctaagagt tatgctggag ataaacttat gagtgcgagt
     5701 tttgacagtg tgagagttta ttatgccatg gacaaggtaa ccggtgctgt agcgagcagt
     5761 gttttcaatg ttacggtgat aacgccaaag gaggttacat atgggaaata tgattctttt
     5821 gcgtataaag gcgatacgtt gcgggttatt tcctggtctg ggtcgggttc agctccaact
     5881 ttggtactga ctgactttga tacaattaat aactcaaatt gccctggcat agatacgaaa
     5941 atttttcgtt gtgcatatat gacgtttaaa attaccgttg catctgatga ttatggttgc
     6001 ccttggatcg catcctttta ttcctataca gatttacccg gatttggaag ctatacggcc
     6061 cctacagtgc ataacacaat atgcccaacg ataccggtca cttcatacga tatctcctgg
     6121 agtgaaaatt atgtcagcca taataaggcg ttacgaattc agtccacggg gtcaacagtg
     6181 accaccacgc tttcgaccta cctgatggaa ggcggcagac tctgtgatgg cagtaatttt
     6241 tctgataatg atggcagagg agcatactgc cgcgcggtat cagaattgtt aacctttacc
     6301 tcttacggtt gtgataaatc aacggtaacg gttacgccaa ctcgtcatcc ggtaacggat
     6361 aaagtactac acgatattgt ggtgaatgtg aataccagta gtgggcaacc gattgattcg
     6421 acgtgtcgtt ttcagtatgt attgaatgaa ctgtagttcg tagtattgct ctgatgcgtc
     6481 ttatcaggcc tacgaagtga attgcaattt attgaattat cacatatttg taggccggat
     6541 aaggcgttta cgctgctccg gcatttgctg ccagcattca gggcaactcc ggcggcaacc
     6601 actcttccac ttcaatcaac accaacaaag ccgcatcacc gccgtactct ttcggtgcct
     6661 ggtgaaatgc catcacatgc ggatgttgtg ccagccacag cggtgtttgt tgcttcaaaa
     6721 tatgcttccc gtgcccatgc atcacgcagg cacaaaacac atgttcgcgg cgacaggcgg
     6781 caatcaacgc gcccagttcc tgcttggcct gcagctgcgt cagaccgtgt aaatccaaaa
     6841 acaattccgg tgaataatcg ccgcggcgca gtttcttcgc ctcaaaatgg ctgacatccg
     6901 ggcgaacata tttcaccgga ccttcggtat ttaataacgg ctgaaactca tcggagaaat
     6961 aatggctggc atcagcctgc tccgggatca agcgtttcac cggcacttcg ctgatttttt
     7021 tacgctgcgg tcggtggaca atcgtgtcct gcttaatctt gcgagtcccc gccatcaact
     7081 ggcgaaacag agcctggtcc tcctcgctaa gtgttgtttt ctttttcatt tatctgtctc
     7141 tatttctaat tttcctacag tttacccgac tcgtagcaca atcgatcgtt ttaaacgtat
     7201 ttttcctcac tcaccgcgcg tgaatgctga tttatcgtca tcttcatggc aaactagccg
     7261 ccgaaattat tgcgatcatg ccctggagga atacgtggat aaaattttcg ttgatgaagc
     7321 agtaaatgag ctgcaaacca ttcaggacat gttgcgctgg tcggtaagcc gcttcagcgc
     7381 ggcaaatatc tggtacggtc acggcaccga taacccgtgg gatgaagccg tacagctggt
     7441 gttaccatcg ctctacctgc cgctggatat cccggaagat atgcgcaccg cgcgtctgac
     7501 ctccagcgaa aaacaccgta ttgttgaacg cgtgatccgc cgcgtcaacg agcgcattcc
     7561 ggtggcttac ctgaccaaca aagcctggtt ctgcggtcat gaattttacg tcgatgaacg
     7621 cgtgctggtg ccgcgctcgc cgattggtga actgatcaac aataaatttg ccggactcat
     7681 tagcaagcaa ccgcagcata ttttagatat gtgtaccggt agcggctgca tcgccattgc
     7741 ctgtgcttat gccttcccgg aagcggaagt cgacgcggta gatatctctc ccgacgcgct
     7801 ggcggttgcc gaacagaaca tcgaagaaca cggtctgatc cacaacgtca ttccgattcg
     7861 ttccgatctg ttccgtgatt tgccgaaagt gcagtacgat ctgattgtca ctaacccgcc
     7921 gtatgtcgac gcggaagata tgtccgacct gccaaacgaa taccgtcacg agccggaact
     7981 gggcctggca tctggcaccg acggccttaa actgacgcgt cgcattcttg gaaacgcggc
     8041 agattacctt gctgatgatg gcgtgttgat ttgtgaagtc ggcaacagca tggtacatct
     8101 tatggaacaa tatccggatg ttccgttcac ctggctggag tttgataacg gcggcgatgg
     8161 tgtgtttatg ctcaccaaag agcagctgct ggcggcccgt gaatatttcg cgatttataa
     8221 agattaagta aacacgcaaa cacaacaata acggagccgt gatggctgga aacacaattg
     8281 gacaactctt tcgcgtaacc accttcggcg aatcgcatgg gctggcgctc ggctgcatcg
     8341 tcgatggtgt tccgccaggc attccgctga cggaagcgga cctgcaacat gacctcgacc
     8401 gtcgtcgccc tgggacatcg cgctatacca cccagcgccg cgagccggat caggtcaaaa
     8461 ttctctccgg tgtttttgaa ggcgttacta ctggcaccag cattggcttg ttgatcgaaa
     8521 acactgacca gcgctctcag gattacagcg caattaaaga cgttttccgc ccaggccatg
     8581 ctgattacac ctacgaacaa aaatacggtc tgcgcgatta tcgcggcggt ggacgttctt
     8641 ccgcccgcga aaccgccatg cgcgtggcgg caggggcgat tgccaaaaaa tatctcgccg
     8701 agaaatttgg tattgaaatc cgcggctgct tgacccagat gggcgacatt ccgctggaaa
     8761 tcaaagactg gtcgctggtt gaacaaaatc cgttcttctg cccggacccg gacaaaatcg
     8821 acgcgttaga tgaactgatg cgcgcgctga aaaaagaggg cgactccatc ggcgcgaaag
     8881 tcaccgttgt tgccagtggt gtccccgccg gacttggcga gccggtcttt gaccgcctgg
     8941 atgccgacat cgcccatgcg ctgatgagca tcaacgcggt gaaaggcgtg gaaattggcg
     9001 acggttttga cgtagtggcg ctgcgcggca gccagaaccg cgacgaaatc accaaagacg
     9061 gtttccagag caaccatgcg ggcggcattc tcggcggtat cagcagcggg cagcaaatca
     9121 ttgctcatat ggcgctgaaa ccgacctcca gcattaccgt gccggggcgc actattaacc
     9181 gctttggcga agaagttgag atgatcacca aaggccgtca cgatccctgt gtcgggatcc
     9241 gtgcagtgcc gatcgctgaa gcgatgctgg cgatcgtttt aatggatcac ctgttacggc
     9301 agcgggcgca aaatgccgat gtgaagactg atattccacg ctggtaaaaa atgaacaaaa
     9361 ccgcgattgc gctgctggct ctgcttgcca gtagcgctag cctggcagcg acgccgtggc
     9421 aaaaaataac ccaacctgtg ccgggtagcg cacaatcgat aggcagtttt tctaatggct
     9481 gtattgtcgg cgctgacacg ttgccgatac agtccgaaca ttatcaggtc atgcgtaccg
     9541 atcagcgtcg ctatttcggt cacccggatc tggtgatgtt tatccagcgt ctgagtagcc
     9601 aggtgagcaa tctgggcatg ggtacggtgc tgattggcga tatggggatg cccgctggtg
     9661 ggcgtttcaa cggcggtcat gccagccacc agaccggact ggatgtcgat atctttctgc
     9721 aactgccgaa aactcgctgg acctccgcgc agctcttgcg cccgcaagca ctggacttag
     9781 tttcccgcga cggtaaacac gttgtctcca cgctgtggaa gccagaaatt ttcagcttga
     9841 tcaaactcgc tgcccaggac aaagacgtca cgcgcatttt tgttaatccg gcgattaaac
     9901 aacaactttg ccttgatgcg ggcaccgatc gcgactggtt gcgcaaagtg cgaccctggt
     9961 tccagcatcg cgcgcatatg catgtacgat tacgttgccc tgccgatagt ctggagtgtg
    10021 aagatcaacc tttaccgcca tcaggcgatg gttgcggggc agaactgcaa agctggtttg
    10081 cacctctaaa accgggaaca acaaagcctg agaagaagac accgcctccg ttgccgcctt
    10141 cctgccaggc gctactggat gagcacgtga tctaatggaa acgtttaata gcctgtttat
    10201 ggtttccccg ctgttgctgg gagttctctt ttttgtcgcc atgctggcgg gatttatcga
    10261 ctcgattgcc ggtggcggtg ggttactcac cattccggca ttgatggctg cgggaatgtc
    10321 tcccgctaac gcgctggcaa ccaataaact gcaagcctgc ggcggctcta tttccgccac
    10381 tatctacttt attcgccgca aagtggttag tttaagcgat cagaaactca atattgccat
    10441 gacctttgtc ggctcaatga gcggcgcact gttggtacag tacgttcagg ctgatgtctt
    10501 acggcagatt ttgcccattc tggtgatttg tatcggcctc tatttcttgc tgatgccaaa
    10561 gctgggtgaa gaagatcgcc agcgccggat gtacggttta cccttcgcgt taattgccgg
    10621 tggctgcgtc ggtttttatg acggattctt tggcccggct gccggatcgt tttacgcgct
    10681 ggccttcgtt acgctgtgcg gattcaacct cgccaaagcc acggctcacg ccaaattact
    10741 caacgcaacg tcaaacattg gcggtctgct gctgtttatt ctcggcggca aagtgatttg
    10801 ggcgacgggc tttgtcatgc tggtggggca gttcctcggc gcgcgcatgg gttcacggct
    10861 ggtgttgagc aaaggacaaa agctgatccg cccgatgatt gttatcgtct cggcggtgat
    10921 gagtgccaaa ttactttacg atagccacgg acaggagatc ctccactggt tggggatgaa
    10981 ctaatgaaca gtacacacca ctatgagcag ttgattgaaa tttttaatag ctgctttgcc
    11041 gatgaattta atacccgtct gattaaaggc gacgacgaac cgatctatct tcctgctgat
    11101 gcggaagtac cgtataaccg aattgtcttt gcccacggct tttatgccag cgcaattcat
    11161 gagatttcgc actggtgtat tgccggaaaa gcgcgccgtg agctggttga tttcggctac
    11221 tggtattgcc cggatgggcg tgatgcccaa acgcaaagcc agtttgaaga tgttgaagtg
    11281 aagccgcagg cactcgactg gttgttctgt gtggcggcgg gatatccgtt taacgtcagt
    11341 tgcgacaatc tggaaggcga cttcgaaccg gatcgtgttg tgtttcaacg gcgggtacat
    11401 gcgcaggtga tggattatct gacaaatggc attcccgagc gtccggcacg cttcattaaa
    11461 gcactacaaa attattatca cacgccggaa cttacggcgg aacagttccc gtggccggaa
    11521 gcgctcaact gaagccggtg ccacgtttta caatgaggaa agaagatgat cgcggagttt
    11581 gaatcacgca ttctggcatt aatcgacggc atggttgacc atgccagtga tgatgagttg
    11641 tttgccagtg ggtatttgcg tggccacctg acattagcca tcgcagaact ggaaagtggt
    11701 gatgaccact ccgctcaggc ggtgcatacg accgttagcc agagtctgga aaaagccatt
    11761 ggtgccggtg aattgtcgcc gcgtgaccag gcgctggtga ccgatatgtg ggaaaacctg
    11821 tttcagcagg cgtcacagca gtaagttgcg gcgtctggag caccatgcct gatgcgacgc
    11881 ttatcgcgtc ttatcaggcc tacacgtcac acgtaggtcg gataaggcgt tcacgccgca
    11941 tccggcaaac actgcgagag cagattaccc cgccttaacc gctttaccct tcaacaattt
    12001 ccgcacccat aaccgattcg ggtttaacgc cgtcagcgta ctggcatcca tcggaatcgg
    12061 ttcgtcgctc atctgcgccg ccagaatctc ggcacacagc ggggcagaac acaaaccgcg
    12121 agaacctaaa gccgcaaaca taaagagatc gtcaaaaacc ggcgcgctta ccgcctcatc
    12181 tttctgctcc gccaacgacg catattccac gagtgttgcc tcataatcgg gaacattgcc
    12241 taccattggc agatgatcgc gggtggcaca acgcacaccg cagcgcgcct ctttatcact
    12301 gacatcaacc tcttttgccc actgtgcctg cgggaaacaa tcaatcaacc gctggcgatt
    12361 ctgctgctga tcgtcctcac tgtacgccgt atcttcgctg ccgcgatgat aactggcacc
    12421 aatacaatga tgttgattcg ccggattttg tggcgtgaga taaccgtcat agcacagcac
    12481 ctgcttcagc tctgccagtt ccggcgttgt cggaatatgg ctgacctgcc cggcaaccga
    12541 atacaccggg agagtcgacg tttggctgaa acggctgatt tgatgcccgt tcgccagtac
    12601 cactacgctg tgtgttgctt gctgatctcc tgcaaaattc aacaaccaac agtcatcctt
    12661 acgggataaa ttctgtaact gatattgata ataaatctgc aaaccctgct gttgcgccag
    12721 ttccagcaca ttacgggtca gttctgctgg gcacagccaa ccaccttgcg gataagtaat
    12781 gccgctgcaa tttgtcgcaa cgcccgtaat ttgttcaacc gcatttgcct caacggctac
    12841 agccagttct gcgggtaaat ccattgacaa catctgtgcg attttatgct ggcttttctc
    12901 atcccagcct aactgcgtga cgccgcacca gtcatgatca aatttaacgg gtaattggtc
    12961 gtaaaaccga cgagcaaaag taaacgcatt agagaaaaag cggtttagcg cctcatcgtg
    13021 tttgcttaat aacggataca gcgccccctg gcgattgccg gaagcaccca gcgcgggggc
    13081 ttcatccgcg caataaagcg ctacctgcca gccgcgccgt aataacgcca gcgacaacaa
    13141 cgcgctggca ataccaccgc cgataatcgc tacttcccgt ttgctgctgc ccgtacgatt
    13201 aaaccacggc gtggagcagg ggagtggtaa tgtctgttcc atcaccccac aaagcatttc
    13261 ccgtttgcgc ccaaagccct tacgtttttg catcgtgaat ccggcttcct gcaaaccgcg
    13321 gcggacaaaa ccggcagacg taaatgtcgc cagcgtgccg cctggacgcg ccaaccttgc
    13381 catggcgtta aacagatttt gcgtccacat atccgggttt ttagctggcg caaagccgtc
    13441 cagaaaccag gcatctactt tttgatttag cgaatcgtcc agttggctgg tcagttcgtt
    13501 aatatcgcca aaccataaat ccagcgtcac gcggccttca ttgagcaata aacgatggca
    13561 accgggcaag ggcattggcc actgcgcctg aagttgttct gcccacggag ccagttccgg
    13621 ccagtgttga tgcgctaagg ctaaatccgc acgggtgagg ggaaatttct caaaactaat
    13681 gaaatgtaag cgttgtaatt gcgcttgcgg atgcgcttcg cgaaactgat caaatgcctg
    13741 ccatagcgtc aggaagttta atccggtgcc gaagccgctc tctgctacca caaacagagg
    13801 atgtggatgc tcaggaaagc gtacctctaa ttggttgcct cccagaaaaa cataacgcgt
    13861 ctcttccagc ccgttatcgt tggaaaaata gacatcgtca aaatctcggg aaacaggtgt
    13921 accctcagca ttaaattcga ggttggcagg ttgtatggag tagtgtttca cgtaagttac
    13981 tcgtcttaca ggcggtggct cgatcttagc gatgtgtgta aggctgcgca aatttctcta
    14041 ttaaatggct gatcggactt gttcggcgta caagtgtacg ctattgtgca ttcgaaactt
    14101 actctatgtg cgacttacag aggtattgaa tgaaacgtgc agtgattact ggcctgggca
    14161 ttgtttccag catcggtaat aaccagcagg aagtcctggc atctctgcgt gaaggacgtt
    14221 cagggatcac tttctctcag gagctgaagg attccggcat gcgtagccac gtctggggca
    14281 acgtaaaact ggataccact ggcctcattg accgcaaagt tgtgcgcttt atgagcgacg
    14341 catccattta tgcattcctt tctatggagc aggcaatcgc agatgcgggc ctttctccgg
    14401 aagcttacca gaataacccg cgcgttggcc tgattgcagg ttctggcggc ggttccccgc
    14461 gtttccaggt attcggcgct gacgcaatgc gcggcccgcg cggcctgaaa gcggttggcc
    14521 cgtatgtggt caccaaagcg atggcatccg gcgtttctgc ctgcctcgcc acgccgttta
    14581 aaattcatgg cgttaactac tccatcagct ccgcgtgtgc gacttccgca cactgtatcg
    14641 gtaacgcagt agagcagatc caactgggca aacaggacat cgtgtttgct ggcggcggcg
    14701 aagagctgtg ctgggaaatg gcctgcgaat tcgacgcgat gggtgcgctg tctactaaat
    14761 acaacgacac cccggaaaaa gcctcccgta cttacgacgc tcaccgtgac ggtttcgtta
    14821 tcgctggcgg cggcggtatg gtagtggttg aagagctgga acacgcgctg gcgcgtggtg
    14881 ctcacatcta tgctgaaatc gttggctacg gcgcaacctc tgatggtgca gacatggttg
    14941 ctccgtctgg cgaaggcgca gtacgttgta tgcagatggc gatgcatggc gttgataccc
    15001 caatcgatta cctgaactct cacggtactt cgactccggt tggcgacgtg aaagagctgg
    15061 cagctatccg tgaagtgttc ggcgataaga gcccggcgat ttctgcaacc aaagccatga
    15121 ccggtcactc actgggcgct gcgggtgtgc aggaagctat ctactctctg ctgatgctgg
    15181 aacacggctt tatcgccccg agcatcaaca ttgaagagct ggacgagcag gctgcgggtc
    15241 tgaacatcgt gaccgaaacg accgatcgcg aactgaccac tgttatgtct aacagcttcg
    15301 gcttcggcgg caccaacgcc acgctggtaa tgcgcaagct gaaagattaa ttcgcagtag
    15361 gccggagtag gtcggataag acgcgccagc gtcgcatccg acgttacgcg ccaatgcggc
    15421 ctccggcact aacgcaaaag ggaacctgat ggttcccttt ttcacatcat tgacaatcgc
    15481 caccagttcc aggcaaactt cccgctttgt cgatttcctt ctgaaaagac gtacgcgtta
    15541 aatcctgcca acgcaccgta accctgaaac cagagagatg agacggggat actcctcgcc
    15601 ttgcgctgca ttctggagta atgcatgact gctgtaagcc aaaccgaaac acgatcttct
    15661 gccaattttt cgctcttccg catcgctttt gcggtttttc tcacctacat gaccgtaggg
    15721 ctgccgttgc cggttatccc gctgtttgtt catcatgaac tgggctatgg caataccatg
    15781 gtcggcattg ccgtcgggat tcagtttctg gctacggtgc tgacgcgtgg ctatgccggg
    15841 cgactggccg atcaatatgg cgcaaaacgt tcggcgcttc aggggatgtt agcttgtggt
    15901 ctggctggcg gcgcgttgct gctggcggcg attttgcctg tctccgcacc gttcaaattt
    15961 gccctgttgg tcatcgggcg tttgattctt gggtttggtg aaagccagtt actgacaggc
    16021 gctctgacct gggggttagg catcgtaggg ccaaaacact ctggcaaagt gatgtcatgg
    16081 aatggaatgg cgatttacgg tgccctcgct gttggtgctc cgcttggcct gttgattcat
    16141 agccattacg gttttgccgc actggcgatc accacaatgg tattaccctt actggcgtgg
    16201 gcctgtaacg gcacagtgcg caaagtaccg gccctggcgg gagaacgtcc atcgctgtgg
    16261 agcgttgtcg ggtttatctg gaaaccaggg ttagggctgg cactacaagg cgttggtttt
    16321 gcggttatcg ggactttcgt ttcgctctac tttgccagca aaggatgggc gatggcgggc
    16381 tttactctta ccgagtttgg cggcgcattt gtcgtgatgc gcgtcatgtt tggctggatg
    16441 ccggaccgtt ttggcggcgt gaaagtggcg attgtctctc tgcttgtaga aacggtgggc
    16501 ttgttgctgc tctggcaagc cccaggtgcg tgggtcgcat tagcgggcgc ggcgttaacc
    16561 ggagccggat gttcgcttat ctttcctgcg ctgggcgtgg aggtggttaa acgcgtcccc
    16621 tcacaagttc gcggcaccgc actgggcggt tacgccgcgt ttcaggatat cgccctcggc
    16681 gtctccgggc cgctggcggg aatgctggcg accacgtttg gttactcttc ggtatttctt
    16741 gccggggcga tctctgcggt gctgggaatt attgtcacga tactgtcatt tcgtcggggc
    16801 taacgaacca gccagaccag catcaggata atcgcgacta acagtatcca cagcgcggga
    16861 cgcgttgcca tattttgcaa cgtgtccatc atcggtggaa aaggattaag cagcggttgc
    16921 atatcacgcg gatcaatccc tttaacccgc cgttgataaa gctgattaag aatatcctcg
    16981 gcctgggcag aagaaagcga tgattgcgca gaaagaccat atttttgctg gatatacgcc
    17041 gataacgctg ccagttcact ggcatctaaa ggttgtttta gcgccatctg tagtgattcc
    17101 agcgtcggcg tattttgctg gcttaacgtc tggcgcgcct gcaaccaggt caccagatgg
    17161 ttaaacagtt tcgctggaat taactcgcca tcgttcaccc cggaaagttc cagcatcgat
    17221 tgccagatct gtttgctggg ttcccccgtt gccgccgcaa gtttggtcac cagctgtttt
    17281 agcgcattgt gctccgccgg taataaagga cggtcggtcg cctcgcgctg ctgtggttgc
    17341 ggaataacca tcttcccttc ctgcaacagg gtgagaatgg tttttaattg ctccggtgag
    17401 agctgattca gcggcgtctg accaaagtta tgacggatat aatccgtgac cgcctgacga
    17461 ttattcccca gacgtaaata ctcccctaat tgcgctaaaa gctggcgggc agaatggctt
    17521 ttttgcgcgg ccagcagacg ttgcgccaga ttatgctcag cggcagggaa gtgacgcgaa
    17581 agcagcggtg aatctcccga cagaccaata tcgtgcctga tacccgccca cagttctgct
    17641 ctttgttgtt gcgtcagtga ggtcactttc gtcattaagc tttccagcga agtacgttgc
    17701 tgactggata aaggctgatt gcccgcgcca gacggcaggt tatctccctg acctggtggt
    17761 tgcccaggag gagggccgga aataggttgt atcattacgt atccttatac ctgaaatctt
    17821 cgcaagtatg cctggccgcg agattatggc acacttgtcc ggttaactct cgtctcatac
    17881 aggtaacaca aacgtgaaaa tccttgttga tgaaaatatg ccttatgccc gcgacttatt
    17941 tagccgtttg ggtgaggtga ccgcggttcc cgggcgtcca atccccgtcg ctcaactggc
    18001 agacgcggat gcgctgatgg tgcgttcggt cacgaaagtg aatgaatctt tgctggcagg
    18061 aaaacccatt aaatttgttg gcactgccac tgcggggacc gaccatgtcg atgaagcatg
    18121 gttgaagcag gcgggaattg gtttttccgc tgcacctggc tgtaatgcga ttgcggtggt
    18181 ggaatatgtt ttctcctcac tactgatgct tgccgaacgc gatggatttt cactgcacga
    18241 ccgtacggtg gggatcgtgg gcgttggtaa cgttggacgt cgattgcagg cgcgactgga
    18301 agcgttaggg attaaaacat tgctttgcga tccgcctcgc gctgaccgtg gggatgaggg
    18361 tgatttccgc tcgctggatg aattagttca gcatgcggat attctgactt tccatacgcc
    18421 actcttcaaa gatgggccgt acaaaacgct gcatctggcg gatgaaaaac tgatccgtag
    18481 cctgaagccg ggagcgattc tgattaacgc ctgccgtggc gcagtcgtcg ataatactgc
    18541 gttgctgacc tgcctgaatg aaggccagaa gttaagcgta gtgctggatg tctgggaagg
    18601 cgaaccggaa cttaacgtgg agttgctgac aaaagtggat atcggcacgc cgcatatcgc
    18661 aggttatacc ctggaaggta aagcacgcgg tactacgcaa gtgtttgaag cctatagcaa
    18721 gtttattggg catgaacagc acgttgcgct ggatacatta ctgcccgcgc cagagtttgg
    18781 tcgcattacg ctgcatggcc cgctcgatca accgacgctg aaaaggctgg tgcatttggt
    18841 gtatgatgtg cgccgcgatg acgcaccgct gcgtaaagtc gccgggatac cgggtgagtt
    18901 cgataaactg cgcaaaaact atcttgagcg ccgtgaatgg tcatctctgt atgtaatttg
    18961 tgatgacgcc agtgcggcat cattgctgtg taaactgggt tttaacgccg ttcatcatcc
    19021 ggcacgttaa tctcttcttc atgctctctg ctgtaacatt ggcagggagc tttgctattt
    19081 ctggagtaaa ccaccatgtc tgaaggctgg aacattgccg tcctgggcgc aactggcgct
    19141 gtgggcgaag ccctgcttga aacgctggct gaacgtcagt tcccggttgg ggaaatttat
    19201 gcactggcac gtaacgaaag cgcaggcgaa caactgcgct ttggtggtaa aacaatcact
    19261 gtgcaggatg ccgctgaatt cgactggacg caggcgcagc tggcattttt tgtcgcaggc
    19321 aaagaagcta ccgctgcctg ggttgaagaa gcgaccaact caggttgcct ggtgatcgac
    19381 agcagcggac tgtttgctct cgaacccgac gtaccgctgg tggtgccgga agtaaacccg
    19441 tttgtactga ccgattaccg caaccggaat gtcatcgccg taccagacag tctgaccagc
    19501 cagctgctgg cggcactgaa accgttaatc gatcagggcg gtttgtcgcg tatcagtgtt
    19561 accagcctga tttcagcctc cgcccagggc aaaaaagcgg tcgatgcgtt agcggggcag
    19621 agtgcgaaat tgctcaacgg cattccgatt gacgaagaag atttcttcgg tcgtcaactg
    19681 gcgttcaata tgctgccgtt gttgccggat agcgaaggta gcgtgcgtga agaacgtcgt
    19741 atcgttgacg aagtacgcaa aatcctgcag gacgaagggc tgatgatttc ggcaagcgtc
    19801 gtccaggcac cggtattcta cggtcatgcc cagatggtca actttgaagc actgcgtccg
    19861 ctggcggcag aagaagcgcg tgatgcgttt gctcagggcg aagatattgt gctctctgaa
    19921 gagaacgaat tcccgactca ggtaggggat gcttcgggta cgccgcatct ttctgttggc
    19981 tgcgtgcgta atgactacgg tatgccggag caagtccagt tctggtcggt ggccgataac
    20041 gttcgctttg gcggcgcgct gatggcagta aaaatcgccg agaaactggt gcaggagtat
    20101 ctgtactaat gtccgaccag caacaactgc cagtttataa aattgcgctg ggcattgagt
    20161 acgacggcag taagtattac ggctggcaac ggcagaatga agtccgcagt gtgcaggaga
    20221 agctggaaaa ggcgctctcc caggtggcga acgaacccat caccgtcttc tgcgccgggc
    20281 gtactgacgc aggggtacac ggtaccgggc aggtggtgca tttcgaaaca accgcgcagc
    20341 gcaaagacgc ggcgtggacc ttgggcgtaa atgcgaattt acctggtgac atcgccgtgc
    20401 gttgggttaa agctgtacct gatgattttc atgcccgatt tagcgccacg gctcgccgtt
    20461 atcgctacat catctacaat catcggctgc gcccggcggt attgagtaaa ggggtaaccc
    20521 atttttacga accgctggac gctgaacgga tgcatcgggc tgcgcaatgc ttgctgggcg
    20581 agaatgattt cacctcgttc cgtgcggtgc agtgccagtc ccgaaccccg tggcgcaacg
    20641 ttatgcatat taatgtcacg cgtcacggtc cttatgtggt ggtagatatc aaagcgaatg
    20701 cctttgtaca tcatatggtc aggaatattg tcggcagcct gatggaagtc ggtgcccaca
    20761 accagccgga gagatggata gcagagttgc tggcggcaaa ggacagaacg ctggcggcag
    20821 caacggcaaa agcggaaggg ctgtatctgg tcgcggtgga ttaccctgac cggtatgatc
    20881 ttccaaaacc gccaatgggc ccgctatttc tggcggacta acgagaataa tgctcggaac
    20941 atttcaggct gtagccaacg tagttacagc ctgaaagatg acgagtacaa ggcataggca
    21001 aatatggacc tgatttattt cctcatcgat tttatcctgc acattgacgt gcatctggca
    21061 gagctggtcg cggagtacgg cgtctgggtt tatgccattc tgtttttgat tctgttctgc
    21121 gaaacggggc tggtggtaac gccattcctg cctggtgatt cgctgctgtt tgttgctggt
    21181 gcattggcgt cgctggaaac taacgatctc aatgtccata tgatggtggt gcttatgttg
    21241 attgccgcta ttgtcggtga tgcagtgaac tataccattg gacggttatt tggtgacaaa
    21301 ctgttcagta acccggattc gaaaatcttt cgtcgcagtt atcttgataa gactcatcag
    21361 ttttacgaga gacatggcgg caaaacgatt attctcgccc gttttgtacc gatcgtcaga
    21421 acgttcgcgc cgtttgttgc gggaatgggc cacatgtcgt accgtcattt cgccgcttat
    21481 aacgtgatcg gcgcactgtt gtgggtactg atttttacct acgcgggtta tttcttcggc
    21541 acgctcccct tcattcagag caaccttaag ctgatgattg ttggcattat ttttgtttca
    21601 atattgcctg gggttttcga atttattcgt cacaaacgcg ctgcggcacg cgccgcaaaa
    21661 taaaaagtaa ctccgcggtt cgaccacttt tttatccaaa gtttcgggct gttatgtttt
    21721 aatgtgcaac attcatggtc tgttgggggc aaaaatggca ttatgcgtcc ccaaagataa
    21781 aactggcatc gaaccaggtt cagacagaaa ggtccctaat gagctggatt gaacgaatta
    21841 aaagcaacat tactcccacc cgcaaggcga gcattcctga aggggtgtgg actaagtgtg
    21901 atagctgcgg tcaggtttta taccgcgctg agctggaacg taatcttgag gtctgtccga
    21961 agtgtgacca tcacatgcgt atgacagcgc gtaatcgcct gcatagcctg ttagatgaag
    22021 gaagccttgt ggagctgggt agcgagcttg agccgaaaga tgtgctgaag tttcgtgact
    22081 ccaagaagta taaagaccgt ctggcttctg cgcagaaaga aaccggcgaa aaagatgcgc
    22141 tggtggtgat gaaaggcact ctgtatggaa tgccggttgt cgctgcggca ttcgagttcg
    22201 cctttatggg cggttcaatg gggtctgttg tgggtgcacg tttcgtgcgt gccgttgagc
    22261 aggcgctgga agataactgc ccgctgatct gcttctccgc ctctggtggc gcacgtatgc
    22321 aggaagcact gatgtcgctg atgcagatgg cgaaaacctc tgcggcactg gcaaaaatgc
    22381 aggagcgcgg cttgccgtac atctccgtgc tgaccgaccc gacgatgggc ggtgtttctg
    22441 caagtttcgc catgctgggc gatctcaaca tcgctgaacc gaaagcgtta attggctttg
    22501 ccggtccgcg tgttatcgaa cagaccgttc gcgaaaaact gccgccagga ttccagcgca
    22561 gtgaattcct gatcgagaaa ggcgccatcg acatgatcgt ccatcgtccg gaaatgcgcc
    22621 tgaaactggc gagcattctg gcgaagttga tgaatctgcc agcgccgaat cctgaagcgc
    22681 cgcgtgaagg cgtagtggta cccccggtac cggatcagga acctgaggcc tgataactga
    22741 taagggcagg gccactggct ctgccctttt gctattctca ccgtaacgaa tcagcggata
    22801 ccatgattat caaacgcact cctcaagccg cgtcgcctct ggcttcgtgg ctttcttatc
    22861 tggaaaacct gcacagtaaa actatcgatc tcggccttga gcgcgtgagc caggtcgcgg
    22921 cgcgtcttgg cgtcctgaaa ccagcgccat ttgtgtttac cgttgcgggt acgaatggca
    22981 aaggcaccac ctgccgtacg ctggagtcga ttctgatggc ggcagggtac aaagtgggcg
    23041 tctacagttc accgcatctg gtgcgttata ccgagcgcgt acgtgtgcag gggcaggaat
    23101 tgccggaatc ggcccacacc gcctcttttg cggagattga atcggcacgc ggtgatattt
    23161 ccctgaccta tttcgagtac ggtacgctgt cggcgttgtg gctgttcaaa caggcacaac
    23221 ttgacgtagt gattctggaa gtagggctgg gcggtcgtct ggacgcaacc aatattgtcg
    23281 atgccgatgt ggctgtagta accagcattg cgctggatca taccgactgg ctgggaccag
    23341 atcgcgaaag tattggtcgc gagaaagcag gcatctttcg cagcgcaaaa ccggcaattg
    23401 tcggtgagcc ggaaatgcct tctaccattg ctgatgtggc gcaggaaaaa ggtgcactgt
    23461 tacaacgtcg gggcgttgag tggaactatt ccgtcaccga tcatgactgg gcgtttagcg
    23521 atgctcacgg cacgctggaa aatctgccgt tgccgcttgt cccgcaaccg aatgccgcaa
    23581 cagcgctggc ggcactgcgt gccagcgggc tggaagtcag tgaaaatgcc attcgcgacg
    23641 ggattgccag cgcaattttg ccgggacgtt tccagattgt gagcgagtcg ccacgcgtta
    23701 tttttgatgt cgcgcataat ccacatgcgg cggaatatct caccgggcgt atgaaagcgc
    23761 taccgaaaaa cgggcgcgtg ctggcggtta tcggtatgct acatgataaa gatattgccg
    23821 gaactctggc ctggttgaaa agcgtggttg atgactggta ttgtgcgcca ctggaagggc
    23881 cgcgcggtgc cacggcagaa caactgcttg agcatttggg taacggcaaa tcatttgata
    23941 gcgttgcgca ggcatgggat gccgcaatgg cggacgctaa agcggaagac accgtgctgg
    24001 tgtgtggttc tttccacacg gtcgcacatg tcatggaagt gattgacgcg aggagaagcg
    24061 gtggcaagta agtttcagaa tcggttagtg ggcacgatcg tgctggtggc gctgggggtg
    24121 attgtacttc cagggctgct ggacgggcag aaaaaacatt atcaggatga gtttgcggct
    24181 atcccgctgg ttccaaaagc gggcgatcgc gatgagcctg atatgatgcc agccgccacc
    24241 caggcgttgc cgacgcagcc gccggaaggc gcagcggaag aggtgcgggc aggtgatgcc
    24301 gcagcaccgt cgctcgatcc ggccactatt gcagccaata acaccgagtt tgaaccggaa
    24361 cctgcaccgg tcgccccacc gaagccgaaa ccggtggagc cgcctaaacc gaaggttgaa
    24421 gcaccacctg cgccgaagcc agaaccgaag ccggtcgtgg aagaaaaagc agcgccaacg
    24481 ggtaaagcct atgttgtgca actgggtgcg ctgaaaaatg ccgataaagt gaatgagatt
    24541 gtcggtaagc tgcgcggtgc cggttatcgg gtttatacgt cgccatccac gccagtgcag
    24601 ggtaaaatta cccgtattct ggttgggccg gatgcctcga aagataagct gaaaggttcg
    24661 ctgggtgagt tgaagcaact ttctggctta agtggcgtgg taatgggcta tacgccgaat
    24721 taatacagtc ttgcctgatg cgacgctggc gcgtcttatc aggcctgggc agtgttagaa
    24781 ccgtaggtcg gataaggcgt ttacgccgca tccgacacgc attgcacgat gccgcaaagg
    24841 cataaaaagt cgatggcgtt gaatattttt tcagcgccat ttttattgat gcgtgggaag
    24901 gaaatcccta cgcaaacgtt ttctttttct gttagaatgc gccccgaaca ggatgacagg
    24961 gcgtaaaatc gtgggacaca tatggtctgg attgattacg ccataatcgc ggtgattgct
    25021 ttttcctctc tggttagcct gatccgcggc tttgttcgtg aagcgttatc gctggtgaca
    25081 tggggttgtg ctttctttgt tgccagtcat tactacactt acctgtcagt ctggtttacg
    25141 ggctttgaag acgaactggt tcgaaatgga attgccatcg cggtactgtt tatcgctacc
    25201 ctgattgttg gtgctatcgt gaacttcgtg ataggccagt tggtggagaa aacggggttg
    25261 tcaggcaccg atcgggtgct gggcgtctgt ttcggtgcgt tgcgcggtgt gttgattgtt
    25321 gccgccattc tcttctttct cgactccttt accggggtgt cgaaaagcga agactggagc
    25381 aaatcacagc tgatcccgca gttcagtttt atcatcagat ggttttttga ttatctgcaa
    25441 agctcgtcaa gtttcttgcc cagagcgtaa gtgctctgag atgtggctta acgaggaaaa
    25501 agacgtatgt gcggtattgt cggtatcgcc ggtgttatgc cggttaacca gtcgatttat
    25561 gatgccttaa cggtgcttca gcatcgcggt caggatgccg ccggcatcat caccatagat
    25621 gccaataact gcttccgttt gcgtaaagcg aacgggctgg tgagcgatgt atttgaagct
    25681 cgccatatgc agcgtttgca gggcaatatg ggcattggtc atgtgcgtta ccctacggct
    25741 ggcagctcca gcgcctctga agcgcagccg ttttacgtta actccccgta tggcattacg
    25801 cttgcccaca acggcaatct gaccaacgct cacgagttgc gtaaaaaact gtttgaagaa
    25861 aaacgccgcc acatcaacac cacttccgac tcggaaattc tgcttaatat cttcgctagc
    25921 gagctggaca acttccgcca ctacccgctg gaagccgaca atattttcgc tgccattgct
    25981 gccacaaacc gcttaatccg cggcgcatat gcctgtgtgg cgatgattat cggccacggt
    26041 atggttgctt tccgcgatcc aaacgggatt cgtccgctgg tactgggaaa acgtgatatt
    26101 gacgagaacc gtacagaata tatggtcgct tccgaaagcg tagcgctcga tacgctgggc
    26161 tttgatttcc tgcgtgacgt cgcgccgggc gaagcgattt acatcactga agaagggcag
    26221 ttgtttaccc gtcaatgtgc tgacaatccg gtcagcaatc cgtgcctgtt tgagtatgta
    26281 tactttgctc gcccggactc gttcatcgac aaaatttccg tttacagcgc gcgtgtgaat
    26341 atgggcacga aactgggcga gaaaattgcc cgcgaatggg aagatctgga tatcgacgtg
    26401 gtgatcccga tcccagaaac ctcgtgtgat atcgcgctgg aaattgctcg tattctgggc
    26461 aaaccgtacc gccagggctt cgttaaaaac cgctatgttg gccgcacctt tatcatgccg
    26521 ggccagcagc tgcgtcgtaa gtccgtgcgc cgtaaactga atgccaaccg cgccgagttc
    26581 cgcgataaaa acgtcctgct ggtcgacgac tccatcgtcc gtggcaccac ttctgagcag
    26641 attatcgaga tggcacgcga agccggagcg aagaaagtgt acctcgcttc tgcggcaccg
    26701 gaaattcgct tcccgaacgt ttatggtatt gatatgccga gcgccacgga actgatcgct
    26761 cacggtcgcg aagttgatga aattcgccag atcatcggtg ctgacgggtt gattttccag
    26821 gatctgaacg atctgatcga cgccgttcgc gctgaaaatc cggatatcca gcagtttgaa
    26881 tgctcggtgt tcaacggcgt ctacgtcacc aaagatgttg atcagggcta cctcgatttc
    26941 ctcgatacgt tacgtaatga tgacgccaaa gcagtgcaac gtcagaacga agtggaaaat
    27001 ctcgaaatgc ataacgaagg atgatgcctt cgctgagggt gccggtctgg caccctgact
    27061 tgcaactccc gccgaaatac tgcaaagtct gcctgcaagt ctgacagggc aactatttat
    27121 gaaacgactc attgtaggca tcagcggtgc cagcggcgcg atttatggcg tgcgcttatt
    27181 acaggttctg cgcgatgtca cagatatcga aacgcatctg gtgatgagcc aggcggcgcg
    27241 ccagacctta tccctcgaaa cggatttttc cctgcgcgaa gtgcaggcat tagctgatgt
    27301 cacgcacgat gcgcgcgata tttccgccag catctcttcc ggttctttcc agacgctggg
    27361 gatggtgatt ttaccctgtt caatcaaaac cctttccggc attgtccata gctatactga
    27421 tggcttactg acccgtgcgg cagatgtggt gctgaaagag cgtcgcccgt tggtgctctg
    27481 cgtgcgtgaa acaccattgc acttaggcca tctgcgttta atgactcagg cggcagaaat
    27541 cggtgcggtg attatgcctc ccgttccggc gttttatcat cgcccgcaat cccttgatga
    27601 tgtgataaat cagacggtta atcgtgttct tgaccagttt gcaataaccc ttcctgaaga
    27661 tctctttgcc cgctggcagg gcgcataact ccgctgttgc cctgtttcag ggcaattttg
    27721 caaccgcgat caaatcctcg acattttgtt tctgccattc aatcgaaacg ctgcgattca
    27781 accgctatac ctgctatctt caacttcagg acaataatgc aacgtcttat taacatattt
    27841 aacgttgaat gttactgttg tcgtcaagat ggcataagac ctgcatgaaa gagcctgcaa
    27901 acacacaaca caatacacaa cataaaaaag ccattttcac ttgagggtta tgtatgaaga
    27961 agtcgattct cgctctgtct ttgttagtcg gtctctccgc agcggcttcc agctatgcgg
    28021 cgctaccgga gacggtacgt atcggaaccg ataccaccta tgcgccgttc tcgtcgaaag
    28081 atgctaaagg tgattttgtt ggctttgata tcgatctcgg taacgagatg tgcaaacgga
    28141 tgcaggtgaa atgtacctgg gttgccagtg actttgacgc gctgatcccc tcactgaaag
    28201 cgaaaaaaat cgacgccatt atttcgtcgc tttccattac cgataaacgt cagcaggaga
    28261 ttgccttctc cgacaagctg tacgccgcag attctcgttt gattgctgcc aaaggttcac
    28321 cgattcagcc aacgctggat tcactgaaag gtaaacatgt tggtgtgctg cagggatcaa
    28381 cccaggaagc ttacgctaac gagacctggc gtagcaaagg cgtggatgtg gtggcctatg
    28441 ccaaccagga tttggtctat tccgatctgg ctgcaggacg tctggatgct gcgttacaag
    28501 atgaagttgc tgccagcgaa ggattcctca agcaacctgc tggtaaagct ttcgcctttg
    28561 ctggctcatc agtaaaagac aaaaaatact tcggtgatgg caccggtgta gggctacgta
    28621 aagatgatgc tgaactgacg gctgccttca ataaggcgct tggcgagctg cgtcaggacg
    28681 gcacctacga caagatggcg aaaaagtatt tcgactttaa tgtctacggt gactgatacg
    28741 tcgctgggaa gctgtacctg atggaatgat catcatggtg cacgccaggt ttgttgcact
    28801 atcgtggtgc attgaaatgc atacttaagc atttttaatg aaaaataata cgtctaacgg
    28861 ggcggaatat tttgccttga tggtcaattt tatggcacga taagtgtaac aaacctgtaa
    28921 atattcccta taaaaagact gtcagttgag gacattatga aaaaactggt gctatcgctc
    28981 tctctggttc tggccttctc cagcgcaact gcggcatttg ctgcgattcc gcaaaacatc
    29041 cgcatcggta ccgacccgac ctatgcgcca tttgaatcaa aaaattcaca aggcgaactg
    29101 gttggcttcg atatcgatct ggcaaaggaa ttatgcaaac gcatcaatac gcaatgtacg
    29161 tttgtcgaaa atccgctgga tgcgttaatc ccgtccttaa aagcgaagaa gattgacgcc
    29221 atcatgtcat cgctttccat tacggaaaaa cgtcagcaag aaatagcctt caccgacaaa
    29281 ctgtacgctg ccgattctcg tttggtggtg gcgaaaaatt ctgacattca gccgacagtc
    29341 gagtcgctga aaggcaaacg ggtaggcgta ttgcagggca ccacccagga gacgttcggt
    29401 aatgaacatt gggcaccaaa aggcattgaa atcgtctcgt atcaggggca ggacaacatt
    29461 tattctgacc tgactgccgg acgtattgat gccgcgttcc aggatgaggt cgctgccagc
    29521 gaaggtttcc tcaaacaacc tgtcggtaaa gattacaaat tcggtggccc gtctgttaaa
    29581 gatgaaaaac tgtttggcgt agggaccggc atgggcctgc gtaaagaaga taacgaactg
    29641 cgcgaagcac tgaacaaagc ctttgccgaa atgcgcgctg acggtactta cgagaaatta
    29701 gcgaaaaagt acttcgattt tgatgtttat ggtggctaat cgccatcggt gaaagaaagc
    29761 ccgctccctt cgggtaaccg gagggagaca agaggcggta attcaccaca cacgacagga
    29821 caggcagcat gttgtatggg ttttcaggtg ttattttaca gggtgcgctc gtcacgctgg
    29881 agctggctat cagctctgta gtgctcgctg taatcatcgg tttaattggc gctggcggta
    29941 agctctcgca aaatcggctt tcggggctga ttttcgaagg gtacaccacg ctgattcgtg
    30001 gcgtgccgga cttagtgttg atgctgctga ttttctacgg tttgcagatt gcgctaaaca
    30061 cggtgacgga ggcgatgggc gtcgggcaga ttgatatcga tccgatggtc gctggtatta
    30121 tcactctcgg ttttatctac ggtgcttatt ttaccgaaac gttccgtggt gcttttatgg
    30181 cagtgccgaa aggacatata gaggcggcga cggcgttcgg ttttactcgt gggcaagtgt
    30241 ttcggcggat catgtttccg gcgatgatgc gttacgccct gccaggcatt ggcaacaact
    30301 ggcaggtgat cctcaaatct accgcactgg tttcgttact cggcctggaa gatgtggtca
    30361 aagccaccca actggcaggc aaaagtacct gggaaccgtt ctatttcgcc atcgtctgtg
    30421 gcgtgattta cctggttttc accactgttt ccaatggtgt gctgctgttc cttgagcgcc
    30481 gctactccgt gggtgtgaag agggctgacc tgtgatcgaa atcttacatg aatactggaa
    30541 accgctgctg tggaccgacg gttatcgctt tactggtgtg gcgatcactc tgtggctgct
    30601 tattttgtcg gtagtgatag gcggagtcct ggcgctgttt ctggcgattg gtcgtgtctc
    30661 cagtaataaa tacatccagt ttccaatctg gttatttacc tatatttttc gcggtacgcc
    30721 gctgtatgtt cagttgctgg tgttctattc cggcatgtac acgcttgaga ttgttaaggg
    30781 aaccgaattc cttaacgctt tcttccgcag tggcctgaac tgtaccgtgc tggcgctgac
    30841 gcttaacacc tgcgcttaca ctaccgagat ttttgctggg gcaatccgtt cggttccgca
    30901 tggggaaatt gaagccgcca gagcctatgg cttctcgact tttaaaatgt atcgttgcat
    30961 tattttgcct tctgcgctgc gtattgcgtt accggcatac agcaacgaag tgatcctgat
    31021 gctgcactct actgcgctgg cgtttacggc cactgtgccg gatctgctga aaatagcccg
    31081 cgatattaac gccgccacgt atcaaccttt taccgccttc ggcattgccg cggtgctcta
    31141 tttaatcatc tcttatgtcc tgatcagcct ctttcgcaga gcggaaaaac gctggttgca
    31201 gcatgtgaaa ccttcttcaa cgcactgaga atacgatgtc cgagaataaa ttaaacgtta
    31261 tcgatttgca caaacgctac ggcgaacatg aagtgctgaa aggggtctca ctgcaagcga
    31321 atgccggaga tgtaataagc atcatcggat cgtcgggatc ggggaaaagt acctttctgc
    31381 gctgcattaa cttcctcgaa aaaccgagtg aagggtcgat cgtggtcaat ggccagacga
    31441 tcaatctggt gcgcgacaaa gacggtcaac tcaaagtcgc cgataaaaat caactgcgct
    31501 tactgcgcac acgcctgacg atggtattcc agcacttcaa tctctggagc catatgacgg
    31561 tgctggaaaa cgtcatggaa gcgccgattc aggtgttggg cctgagcaag caggaagcgc
    31621 gcgagcgggc ggtgaagtat ctggcgaaag tcgggataga cgaacgtgcg caggggaaat
    31681 atccggtgca tctttccggc ggtcagcaac agcgtgtttc tatcgcgcgg gcgctggcga
    31741 tggaaccgga agttttactg tttgatgaac ctacctcggc gctcgatcct gaactggtag
    31801 gcgaagtgtt acgtattatg cagcaactgg cagaagaggg gaaaaccatg gtggtagtga
    31861 ctcacgaaat gggctttgct cgccatgttt ctactcacgt cattttcctc catcagggga
    31921 aaatagaaga agagggcgcg ccggagcagt tatttggcaa cccgcaaagc cctcgtctgc
    31981 aacagttcct taagggatcg ctgaaataat ttcctctccg gtggctaacg ctgccggagt
    32041 attttcatgg ggaatttgcg agtcagcttc cgctgaaatg taaaagttgc agtccgctaa
    32101 aaatacttct gaattcgctg tccaaagata aaaactgcct gctgcattgg gtgttaatcg
    32161 tggttattgt cgcgtcatct taaatttgag gggtgacgga acgccatgac aatatcgaca
    32221 acttccacgc cgcatgatgc ggtatttaaa tcttttttac gccatccaga
//
LOCUS       DAEEKQ010000027        31835 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.027, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000027 DAEEKQ010000000
VERSION     DAEEKQ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.027"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(1..2588)
                     /gene="acrF"
                     /locus_tag="KLK57_001291"
                     /pseudo
     CDS             complement(1..2588)
                     /gene="acrF"
                     /locus_tag="KLK57_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
     gene            complement(2600..3757)
                     /gene="acrE"
                     /locus_tag="KLK57_001292"
     CDS             complement(2600..3757)
                     /gene="acrE"
                     /locus_tag="KLK57_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HBE0891713.1"
                     /translation="MTKHARFFLLPSFILISAALIVGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDECTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            4156..4818
                     /gene="acrS"
                     /locus_tag="KLK57_001293"
     CDS             4156..4818
                     /gene="acrS"
                     /locus_tag="KLK57_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HBE0891714.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTFNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDGMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIIDGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(4821..5000)
                     /locus_tag="KLK57_001294"
     CDS             complement(4821..5000)
                     /locus_tag="KLK57_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HBE0891715.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVYP
                     LKLVNCDELNFQDRM"
     gene            complement(5084..5968)
                     /gene="yhdJ"
                     /locus_tag="KLK57_001295"
     CDS             complement(5084..5968)
                     /gene="yhdJ"
                     /locus_tag="KLK57_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HBE0891716.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKLPTESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIVECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(6054..6350)
                     /gene="fis"
                     /locus_tag="KLK57_001296"
     CDS             complement(6054..6350)
                     /gene="fis"
                     /locus_tag="KLK57_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HBE0891717.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(6376..7341)
                     /gene="dusB"
                     /locus_tag="KLK57_001297"
     CDS             complement(6376..7341)
                     /gene="dusB"
                     /locus_tag="KLK57_001297"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HBE0891718.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(7670..8551)
                     /gene="prmA"
                     /locus_tag="KLK57_001298"
     CDS             complement(7670..8551)
                     /gene="prmA"
                     /locus_tag="KLK57_001298"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HBE0891719.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     IVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYVDSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(8563..10014)
                     /gene="panF"
                     /locus_tag="KLK57_001299"
     CDS             complement(8563..10014)
                     /gene="panF"
                     /locus_tag="KLK57_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HBE0891720.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(10004..10246)
                     /locus_tag="KLK57_001300"
     CDS             complement(10004..10246)
                     /locus_tag="KLK57_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HBE0891721.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(10355..11704)
                     /gene="accC"
                     /locus_tag="KLK57_001301"
     CDS             complement(10355..11704)
                     /gene="accC"
                     /locus_tag="KLK57_001301"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HBE0891722.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     RGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(11715..12185)
                     /gene="accB"
                     /locus_tag="KLK57_001302"
                     /gene_synonym="fabE"
     CDS             complement(11715..12185)
                     /gene="accB"
                     /locus_tag="KLK57_001302"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HBE0891723.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(13163..14137)
                     /locus_tag="KLK57_001303"
     CDS             complement(13163..14137)
                     /locus_tag="KLK57_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE0891724.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            14289..16229
                     /gene="csrD"
                     /locus_tag="KLK57_001304"
     CDS             14289..16229
                     /gene="csrD"
                     /locus_tag="KLK57_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HBE0891725.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            16534..17577
                     /gene="mreB"
                     /locus_tag="KLK57_001305"
     CDS             16534..17577
                     /gene="mreB"
                     /locus_tag="KLK57_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HBE0891726.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            17643..18746
                     /gene="mreC"
                     /locus_tag="KLK57_001306"
     CDS             17643..18746
                     /gene="mreC"
                     /locus_tag="KLK57_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HBE0891727.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            18746..19234
                     /gene="mreD"
                     /locus_tag="KLK57_001307"
     CDS             18746..19234
                     /gene="mreD"
                     /locus_tag="KLK57_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HBE0891728.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            19243..19836
                     /gene="yhdE"
                     /locus_tag="KLK57_001308"
     CDS             19243..19836
                     /gene="yhdE"
                     /locus_tag="KLK57_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HBE0891729.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            19826..21295
                     /gene="rng"
                     /locus_tag="KLK57_001309"
     CDS             19826..21295
                     /gene="rng"
                     /locus_tag="KLK57_001309"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HBE0891730.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            21363..25163
                     /gene="yhdP"
                     /locus_tag="KLK57_001310"
     CDS             21363..25163
                     /gene="yhdP"
                     /locus_tag="KLK57_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HBE0891731.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWHYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQYITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            25319..26764
                     /gene="tldD"
                     /locus_tag="KLK57_001311"
     CDS             25319..26764
                     /gene="tldD"
                     /locus_tag="KLK57_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HBE0891732.1"
                     /translation="MSLNLVSEQLLAANSLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(26892..27757)
                     /locus_tag="KLK57_001312"
                     /pseudo
     CDS             complement(26892..27757)
                     /locus_tag="KLK57_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
     gene            27940..28143
                     /locus_tag="KLK57_001313"
     CDS             27940..28143
                     /locus_tag="KLK57_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HBE0891733.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            28151..29083
                     /gene="aaeA"
                     /locus_tag="KLK57_001314"
     CDS             28151..29083
                     /gene="aaeA"
                     /locus_tag="KLK57_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HBE0891734.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGGRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            29089..31056
                     /gene="aaeB"
                     /locus_tag="KLK57_001315"
     CDS             29089..31056
                     /gene="aaeB"
                     /locus_tag="KLK57_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HBE0891735.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVFAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            31148..31420
                     /locus_tag="KLK57_001316"
     CDS             31148..31420
                     /locus_tag="KLK57_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HBE0891736.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(31476..31739)
                     /gene="yhcN"
                     /locus_tag="KLK57_001317"
     CDS             complement(31476..31739)
                     /gene="yhcN"
                     /locus_tag="KLK57_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HBE0891737.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDTAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         7677 a         7999 c         8363 g         7796 t
ORIGIN      
        1 tcctgatacg acatacctgt ccagtcataa ccaatgcccg caggtaattt tgacgcaagg
       61 ttttccatca atgccatggc atcgccggaa ctggttcctg gcgcggcttc cccctgaatc
      121 tccattgacg gcagaccgtt gtagcgttcc agtcgcggag agccatacac ccaatgtgaa
      181 gtggtaaagg cagagaatgg caccatttcg ccgttggcgc tgcggacata aagtttatcg
      241 acatcttctg gcagcatacg gaatttggca tccgcctgaa catacacctt tttcacgcga
      301 ccacggtcga tgaagtcgtt aacgtaagtc ccacccagcg ccgttgaaat ggtctgattg
      361 atgtcagaaa gtgaaacacc taatgcctgc gccttttcct ggtcaacttc cagtttaaac
      421 tgcgcggtgt cttccagacc attaggacgc acgctgacta agctggcagg atgttgcgcc
      481 gccatgccaa gcaactggtt acgggcctgg gtcagggcat cgtgacccag cccagcctga
      541 tcaattaact caaagtcgaa acccgttgcc gtgcccagtt caacaatggc tggcatattg
      601 aatggaatga caaaaccgtc gcggatcttg cccaattcca ttttggcacg atggattacc
      661 gcttccgcac tgttttcgtc accactacgc tcttcccacg gtttcagact gacgaaagcc
      721 ataccggcgt tttgtgcctg gccgctgaag ctaaagccgt taaccgtaaa gacactttct
      781 acgttcgctt tctcgttatt cagatttgat ccaacacttt ttgcgtccgc tcttgcgtcg
      841 cgccagcggg taactgaatc atggtcagaa agacaccctg atcctcttca ggtaagaagg
      901 aagaaggaag acggaagacg taaaaacaac accaccattc ctgcaacaat cagcgcatag
      961 atcagtaaat atcgtcctgt ggaaccgagg attttgccga cgctgttggt gtagtggtta
     1021 acgctatgat cgaaggtggt attaaaccag ccgaagaaac cgcccttatt ttcgtgatgc
     1081 tcagcagaga cgggtttaag cagcgttgca cataacgcag gggtaagaat caatgccacc
     1141 agaacagaaa gcgccattgc cgaaacgatg gtgatagaga actggcgata aattgcccca
     1201 gtagaaccgc cgaagaatgc catcggaata aataccgctg acagcaccat cgcgataccc
     1261 accagtgcgc cctgaatttg cgacatcgat ttttccgtcg cttcttttgg cgggagctta
     1321 tcctccatca tcacgcgctc gacgttctcc accaccacta tcgcatcatc gacgagcagc
     1381 ccgatggcaa gcaccatccc gaacatcgtt agtgtgttga tggagtaacc aaaagcggcg
     1441 aggatggcaa acgtccctaa caacaccacg ggtaccgcaa tggtggggat cagcgttgct
     1501 cgcatattct gcaagaacag atacatcacc aggaacacca gcataatggc ttcgaacagc
     1561 gtttttacca cttcgtgaat agaaagctgg acgaatggcg tggtgtcata agggtagaga
     1621 accttcattc cctgcgggaa gaatggctgt aattccgcca gttttgcctt aatggctttc
     1681 gcggtattga gagcgttcgc gccggttgct agcttaatcc ccaggcccgc cgccggtttt
     1741 ccgttgatac gagcgataac gttatagttt tcaccgccaa gttcaactcg tgcgacatct
     1801 ttcaggcgta ccaccgagcc gtcactgttt acgcgcaggg ttactttgcc gaattcttcc
     1861 ggatttttta accgcgtctg agcaataatc gaggcgttca actgttgccc tggtaacgct
     1921 ggcgttccgc ccaactgccc ggcagcgatc tgatcgttct gtaccttcaa ctggttaatc
     1981 acatcaaccg gtgtcagttt atatttgttt agcagatcgg cgtccagcca gatacgcatc
     2041 gcatactgtg cgccgaaaag ctgtacgtca ccgacgccat tcagacggct aagcgtatct
     2101 ttaacgttag aggccacata gtccgagata tcgtcctgtg tggtgtctgg gttatcagag
     2161 acaaagcccg ccaccatcaa atagctgcta ctggactttt caacactgat cccctgctgc
     2221 tgaacctcct gcggcagcaa cggcgtggcg agctggagtt tgttctgcac ctgcacttgc
     2281 gcgatatcag gatcggtccc ggactggaag gtaagggtaa ttgtcacgct accggcggaa
     2341 tcgctggtgg aggacatata catcaggtta tcgataccat tcatattctg ttcgataacc
     2401 tgcgtcaccg tatcctgcac ggtctgcgca tcagcacctg gatagtttgc ctaaacagaa
     2461 accgcaggcg gtgcaatggt tggatactga gcgacgggca attgtaggat cgccagtgcg
     2521 cccgccatca tcagaataat ggccagcacc catgcaaata tcggtcgtcg aataaaaaag
     2581 tttgccatgt cagattacct tacttcgatg cagtatctgc gggggtatcg gtagtggctt
     2641 taacctgctc tcccggacgc gctttttgca ggccgctgac aatgacttga tcgccagatt
     2701 tcagaccttc gctaatcaac catttatcgc caatcgcctg actggcaacg acagggcgcg
     2761 cttccacctg acttttatcg ttaacaatca gcacggttgc atcaccacgc ggtgtgcggc
     2821 taacgccttg ttgcgggata agaatggcgt caggttggac gccttcatca atccgtgcac
     2881 gcacaaacat acccggcaaa agcgtatgtt gcgggttagg gaagacagca cgtagggtta
     2941 tggagccggt gcattcatca acggtcacat cagagaattg cagcgtacct ttcaggggat
     3001 aggtttgacc gttttccatg accaactcta cgttgctggt ggcgttttcc ttatgcaaat
     3061 ttccttgctc tacggattgc ttcagcctca taaaatcgtt gctggattgg gtcacatcaa
     3121 cgtagatagg atcgagctgc tggacagtcg ccagttcagt cgtttgccca ttagtgacaa
     3181 gagcgccttc ggtcacggtc gatttgccga tacgtccgct aattggcgca gtcactttgg
     3241 tataagcgag attgatgcga gcgctttcga ctgtggcttt tgcggcaatc acggcggcat
     3301 cggcctggcg agcatcagca atggcctggt cgtactcctg ctggctgatg tatttcgtac
     3361 ccacgagcgg aacgtaacgt tttaccgtca aatgcgcgat ggcggcggcg gcttcacttt
     3421 tcgccagttc gcctttcgcg ctgtcataat ttgcctgata ggtcgcggga tcgatctggt
     3481 acagggactg gcctgcttgt acatcgctgc cttcagtgaa attacgattc agtacgatcc
     3541 cgctaacctg tgggcgaact tcggctatac ggtaagcatt ggtgcggcct ggtaattcag
     3601 tcttaacttc taacggggcc gttttcacaa tatgaacggt aacctgcggt tcaccgacgt
     3661 gagctttctc ttctccctta tcgttacaac cgacgattaa agccgcggag atcagaataa
     3721 aggagggcag gaggaaaaac ctggcatgtt tcgtcattac tattcctcaa aaaaacaaaa
     3781 gcgcgttatt tacccagaaa ggcaacgcgt taactcgcag aaagaaaaat acagtttgct
     3841 atcctacaaa ttatcattcg tcgatgtaag gaatagttat gaatactggc gtctcaaggc
     3901 acataaacac aaaaaaagat taatattcta ctgttttatt ttgacgcggg ttgaaagagg
     3961 cagaattaaa acctcgtaaa ttgaaatata tattcatgtg gcgaatgtat tttaggtaaa
     4021 taatatatat tatttatttg acgatttata gcaactcaat gttaccctct cgtataaata
     4081 cacattagat gatagattaa ccttcgctat tttctcaccc tgtgtcgaat atatttattt
     4141 cctgaataat taatcatggc aaaaagaacc aaagccgaag ctctgaagac ccggcaagaa
     4201 ctgattgaaa ctgccatcgc ccagtttgcg caacatggcg taagcaagac gacgttcaac
     4261 gacattgccg atgccgctaa cgttacgcgt ggcgctatct actggcactt cgagaacaag
     4321 acccaactgt ttaatgagat gtggttgcaa cagccttcat tgcgggagtt aatccaggaa
     4381 cacttgacgg ctggattaga gcatgacccg tttcaacaat tgcgtgaaaa attgattgtc
     4441 ggcttgcaat atattgccaa aattccccgc cagcaggcgt tgctgaaaat cttatatcac
     4501 aaatgtgaat ttaatgatgg gatgctggcc gagggagtga tacgcgaaaa gatgggcttt
     4561 aatccgcaga ctctccgcga agtattgcag gcgtgtcagc aacaaggttg tgtagcaaat
     4621 aacctcgatt tagatgttgt gatgattatt attgatggtg ccttcagcgg aattgttcaa
     4681 aactggttaa tgaatatggc gggttatgat ctttataaac aagcacccgc gctggtcgat
     4741 aacgtattaa gaatgttcat gccagatgaa aacataacga aattaattca tcaaacaaat
     4801 gaattaagtg tcatgtaaat tcacattctg tcctgaaaat tcagttcgtc acagttaacc
     4861 agttttagtg gataaactga gttcatatta cggcatttgt ctgtttctgt tgtcagcagt
     4921 tcaatccact gcatcagtaa agtatcaaac agaaaaacga agacagcgaa aacgacgagc
     4981 caccaatact tgcgaatcat tacctgagcc tgtgcatgtt taaccaagtc tggcaggaaa
     5041 tatacacgaa atgaataatc tgaaaaccag gcttacactt cctttacttt gcaatgagat
     5101 cggggtcaac ttcgcttaac cggcttcgtt ttgacaggtt gccggtcttt ctttttttca
     5161 ctttcgccag ttcttccgcg gagtaatgcg acgcgacatc cagccgtcga agccccattt
     5221 tgatgtactc gctgttgatc tcaataccaa tgaattttcg tccgctggcg atggctacgg
     5281 caccggtagt aaagctaccg gcaaacgggt cgagaacgat atcgcctggg ttggaagagg
     5341 cgagaataat gcgtttcagt aaggcttccg gtttttgcgt cgggtggttc tcatattcat
     5401 ccatcaaata acgcacgcgc ggaaaatccc agacgttacc cggtactttt tgatgattgt
     5461 atggctgtgg aggatttttg cgataatcga tcaacgcgcg ctgcgatccg gttttggctt
     5521 ctaccagaat agcatcaccg ttgaatgtgt agttctttgc gtctttcacc atcatcagga
     5581 tgggttcgta catggagccg tagtgttttt tcgcctgtac tccagaactg tcatatgacc
     5641 agacgatgcg acttttgatg gtaaaaagct tgcggcactg gagatcgata aagggcatgt
     5701 tttccgtact gttcataatg tacatgctgc cctgcttttt cagaacgcgg tggcactcta
     5761 caatcacttc aaacagccag tcgataaaca gatcttcttt ccaggcttcg atcagaccat
     5821 caaaattttt accgatgtta tacggtgggt cggcaaagat cagatcgacg ctttcagtag
     5881 gtagcttttt aagttcggca agggcatcac cgtgaataat ggtcttagct tcattaccaa
     5941 accgggtcgg ttcacatcct gttctcatgg tcactccctt tgtgacacct ataaaaaagg
     6001 cgcttcccca tgccgagtag cgccttttta atcaagcatt tagctaacct gaattagttc
     6061 atgccgtatt ttttcaattt tttacgcagc gtaccacggt tgatgcccat catcagcgca
     6121 gcacgggtct ggttaccacg ggtgtattgc atcaccatgt ccaacagggg ctgttctact
     6181 tcagccagta ccagctcata gaggtcattc acatcctgac cattcagttg agcaaaatag
     6241 ttcttcagtg cctgtttaac cgagtcacgc aggggttttt gggttacctg atcctgagag
     6301 ttaacggtag aaacggtcag tacgtcagaa tttacgcgtt gttcgaacat agttctgtca
     6361 gctctttatt tctgtttacg caaaattttc gaagtatgcc tccaacgcct ccagctgttc
     6421 gctggcatcc tcaatggcgt tgaatgtgcg ccgaaactgg tcatttggag cgtgttcctg
     6481 gagataccag gaaacgtgtt tacgtgcaat tcggtaccct tttgccggac cataaaagtc
     6541 atgcagttcc cgaacgtgcg cgcaaagcaa gcgcttaacc tctgccaaag gcagcggggg
     6601 cagcaactcc ccagtgtcca gataatgctg gatttcccga aagatccagg gtcttccctg
     6661 agctgcgcgg cctatcatca gggcatccgc ccctgtatag tcgagcacag ctctggcttt
     6721 aagcgggtca gtaatgtcgc cattcgcgat aaccggaatg gaaactttct gcttaactgc
     6781 ccgaatactg tcgtactcag cttctccatt gaacaaacag gcgcgtgtac ggccatgaat
     6841 ggtcagagcc tgaatgccac agtcttcagc cagttgggca atctcttcgc agttacggtg
     6901 ttccggtgcc cagccggtgc gaatcttcag ggtaacagga acgtccactg cattgacgac
     6961 ctcggtaagg atcgatttaa cgacatccgg gtactgcaag agggctgaac ctgcgagctt
     7021 gcgattcact tttttagccg ggcaacccat attgatatca ataatctggg caccgctttc
     7081 cacgttaata cgtgctgcat ctgccatttc tttcggatcg ctaccagcaa tttgcacggt
     7141 gcgaataccg ggttcatcaa tgtgcaccat ccgtaaacga gatttgtcgc tttcccaaac
     7201 ctgtgggtta gaagacatca tctcggatac tgtcaatccg gctcccatct cgtagcacaa
     7261 cgtccgaaaa ggtctgtctg taatgccagc catgggcgct gcgatcaggc gatttctgag
     7321 ctgatattgt ccgatgcgca tgagttaaga aatgaccata ctgtgactgc aaggcggcgt
     7381 atattacgca ttttttgcac gagatgaaag gccaaacttt gaccaatcct ctgagatgga
     7441 tcaaagaatt gcatttaaaa tgagcgtggt gagataatta ctcataaaaa tcatcatatt
     7501 agaaaatagt gactaaaatt tacactcaaa gaaatttgag taagttctca atttttcttt
     7561 atgaatgaaa attttggcac gcaaatttgc gtaaataacc ggcaaattta cgtgcctttt
     7621 gtgagcttgc tcgcacttcg ccccgcgtca ccctacggcg atgcgaaggt taattcttac
     7681 gaccggtaat gcggcaccac tcttcttttt ccacgaccgg gtccagtgcg aagctgtcga
     7741 cataagcttc acaaacgctc tctgcctggc ttgccagaat accggaaagg cccagcaaac
     7801 cgcctgaaac cggcaggacg ctgattaacg gtgccagttc acgtaatggg cctgcaagga
     7861 tgttagcgac caccacgtcg gctttcattt cttctggctg atctttcggt aagtagagtt
     7921 ccagacggtc agaaacgcca ttacgttcgg cgttgtcgcg gctagcctga atcgcctgcg
     7981 gatcgatatc aataccaatg gcttttgctg cacccagttt cagcgccgcg atagccagaa
     8041 tgccggaacc acagccaaag tcgatgacta ttttaccggt taaatcgagg ctgtcgagcc
     8101 actgcaggca cagagaggta gttggatggg tacccgtacc aaacgccagc cctggatcta
     8161 acatcacgtt gacggcgttt tcgtccggca catcacgcca gctagggcag atccacagtc
     8221 gttcaccaaa gcgcatcggg tggaaattat ccatccattc gcgctcccag tctttatctt
     8281 ccagttgttc gattttatgc gcgaagcctg cgccgagcag cggatggttt tccagaatcg
     8341 ccaccacgtc gttcatatcg gtttcagcgt cgaacagacc aatcacatcg gtgtcgcccc
     8401 acaggcgcgt ttcgcccggc agcggctcaa atactggcgt atcgtgggta tcctgaaaag
     8461 tgatagaaac ggcacccgct tccatcagcg catcgctaag atcttcagcg ttcgcgccgg
     8521 tggtgttcag tttcagttgg atccaaggca tggcaaaact ctttatttat cagtagtcaa
     8581 aacggtagct tgcgggacgg atgtaccgaa acggtttccg accaggaaag ccagcaaact
     8641 tagtagtaac gagggcacga tagggtggaa gcccaggtac tgaatattca gcgtcgcgag
     8701 tacggcatac agcacgccgc caacgatcat cgcacttagc gcgcctttgg cgttggcgcg
     8761 ttcccagtaa agacccagca ccagcggcca caggaaaacg gcttccagcc caccgaaggc
     8821 cagcaaattc agccagatga tcatttctgg cggcttccag gcggcaagca gcagcaacgc
     8881 gccgagaact aacgtaatta ccgccgacat ccgcttcaga cgcgtctcgt tttgcatttg
     8941 atccggacgg atattcagat agagatcttt aatgatcgta gcggaacttt gtagcaattg
     9001 ggcgttaatt gtcgacatga tcgcagccat aggtgcagcc agaaagatcc cggcagcaaa
     9061 cggtggcagc acttttacca ttaacgtcgg gatcaccaga tccggtacgg tgagatcggg
     9121 gatcactgca cgacctaacg ctccggctag gtgcataccg aacatcagga ttgcgactac
     9181 aatcgtaccg atgatgatcc cccggtgtac cgctttgctg tctttataag agatacagcg
     9241 caccgcagta tgcggcaggc caatcacgcc aaagcacacc agtacccaga acgacgtcat
     9301 aaaggcaggc gacagaatat cgtcagcgcc ttgtggcgta accagttgcg gatcgatggt
     9361 ttgcaaggtc tgtactgcgt tgcttaagcc gccagcggca tgtaccacgc caataagcag
     9421 cacaacggtg ccaatcagca tcacaagccc ttgcatggtg tcgttcagca cgctggcgcg
     9481 aaagccgcca aaggcggtat acaacgcaat gctgataccg aaaatcagca gcccggtttc
     9541 ataaggaata cccgccgcgg tttccagcag gcgcgcaccg ccgataaact gcacggtcat
     9601 tgccccaacg aacgcaacca gcaaactcaa actcgccagc cacaccagaa gacgactctg
     9661 gtagcgggca aacagcatgt cgttcagcgt cactgcattg tagcggcgcg caagaatcgc
     9721 aaacttcttg ccgagaatac cgagtgaaag ccagactgca ggaagctgaa tcatcgccag
     9781 caatacccag cccagcccgt atttataagc agctcctggc ccgccgataa acgaactggc
     9841 actgatatag gtcgcggtga gcgtcatcgc cagcacaata ccgcccatag agcggctgcc
     9901 gaggaaatac tcattaagga aggtgccggt gctccgttta cgcatcgcat aaaccgagat
     9961 accgaacacc accaccagat aggcgaccag cggtagaatt acttcaagct gcatcgtcat
    10021 cctccagtgg gatatcgcga tagataaatt tcaccatcgc ccagcacagt ccaataaaca
    10081 gtagcggcgt caggatgcag gccatctcaa accagcgcgg aaagccggta aaaccggggg
    10141 caacgccagg taagtaagcg gctactaacc aaactgccag atacaaaagg gtcagcccca
    10201 gcgcccagcg cgcctcttta tgggcctgaa caaaacgagt gtccattttt tgtccctgat
    10261 gggtgaagaa agcgaggatt gtaccttatg ggggttgtcg atccccagta ataaaaaagg
    10321 ccggaaaatc cggccttttg gcgcttttgc agtcttattt ttcctgaaga ccgagttttt
    10381 tctccagata gtggatgtta gtgccaccat gctggaagtt ctcgtcattc atgatgcgga
    10441 tctgcagatc aacgttggtt ttgataccgt cgatgatcag ctcctgcagc gcattcttca
    10501 tgcgggcaat cgccacgtca cggttttcac cgtagcaaat cagcttaccg atcattgagt
    10561 catagtacgg cggtacggtg tagcccgcgt agatatgaga ctcccaacgt acgccaaaac
    10621 cgccaggtgc gtggaaacgg gtgattttgc ctgggcttgg caggaaggtg ttcggatctt
    10681 cggcgttgat acgacattcc accgcatggc cgcgaacgtg aacttcttct tgcttgatcg
    10741 acagcggttg accggcagcg atacggagct gttctttgat caggtcaacg ccagtgatca
    10801 tttcggtaac cgggtgttct acctgaatac gggtgttcat ttcgatgaaa tagaactcgc
    10861 cgttttcgaa cagaaactcg aaagtacctg caccgcgata gccgatatca acacacgctt
    10921 tagcgcaacg ttcgccgatg tagcgacgca gttccggggt aatgcccggt gctggcgctt
    10981 cttcgaccac tttctggtgg cggcgctgca tggagcagtc acgttccgcc agatagatag
    11041 cgttgccccg accgtcagcc agtacctgaa tctcgacgtg gcgaggattt tccaggtatt
    11101 tctccatgta aaccatatcg ttgctgaaag cagctttcgc ttccgcacgg gtcatggaga
    11161 tggattgtgc cagttcagcg tcgccgcgca ctacgcgcat accgcgacca ccgccgccgc
    11221 cggaggcttt gataatcacc ggataaccaa tgcgtttagc aatggcacgg tttttatcca
    11281 tatcgtcgcc caacgggccg tcagaacccg gtacgcaagg gacgcccgct tttttcatcg
    11341 ccgcgattgc ggatactttg tcgcccatca ggcgaatggt ttctgctttc gggccgatga
    11401 agataaagcc ggagcgttca acctgctcgg caaagttggc gttctcggag aggaagccgt
    11461 aacccggatg gattgctact gcgccggtga tttcagcggc gctgatgatt gccgggatgt
    11521 tcagataact ttttactgac ggagcagggc caatacagac cgtttcatct gccagtaata
    11581 cgtgttttag atcgcgatcc gcgctggagt gcacagcgac agtcttgatg cccagttctt
    11641 tacaggcacg aagaatacgc aatgcaatct cgccgcggtt ggcaataaca attttatcca
    11701 gcatgttcgc ctcgttactc gatgacgacc agcggctcgt caaattctac cggttgtcca
    11761 ctttcgacca gaattgcttt cacggtaccg gatttgtccg cttcgatctg gttcatcatt
    11821 ttcatggctt caacgatgca cagggtatcg cccacgttga ctttctgacc cacttcgatg
    11881 aacgcttttg cgtccgggct tggggtgcgg tagaaagtac caaccatcgg ggaacgtacg
    11941 atgtgaccac tgatttccgc tgctgctggc gcttccatgg aaggaacggt cgccggagcg
    12001 gctgcgttag attgagctgg ctgctgcatc attggtgcag cgtaagcttg ttgcatcaca
    12061 gggaaacttg cggcaggagc tgcacggcta atgcgtactg actcttcgcc ttcagaaatt
    12121 tccagttcgg agatgcctga ttcttcaacc agctcgatca gttttttaat cttacgaata
    12181 tccatgagtg ggttccgtac tctttgttta gtgtgattgt gacaggcgtt tcaccgccgt
    12241 ctgtaaagcg tatgccccga cagctgtatg catagcgata aattccagca ggccgggacg
    12301 ccggtctatt ttgccccaga gtatcaatgt tagacttgac ggacattgtg cagcgatctg
    12361 ctgctatcct ccggcaaaaa acaaaatata ccttcgttgc gccaatgtca ccttttccgc
    12421 agcgcaaaaa ctgcgtcagg gaggacgagg tcgcacatta taacgatttc gtagcaattg
    12481 gcagctaaat actggtctca tcagggaaga taatcaacag ctaacatgta aataaccttc
    12541 aacaccgtgt aatttgcaac aagccgcaca attcacgaaa ttagcgccat catcgacgga
    12601 acttcttata acgtaaggtg aaaagctgta aagccagccc tgcgaagatg aggggctgca
    12661 atgagataat cttcacagac cacaaataat gtagggatgc caggatcgcg acaagataga
    12721 cgacattacg cagcggttgc tggggattac ccaattttca ctgcatcgtc tggatagatg
    12781 tgaatgctaa agcacgcaaa atcaaccgac tgacaattct cagcattaaa taggttcgag
    12841 tggttaattt gtagtcaaat aaaatcagat tattcacccc atggtaccag aaatcagtaa
    12901 caggttaagc gtagtgtcgc ccaggcaaag caccataaac ctaacaggcg acggaggcgt
    12961 atcaataatg gctgtttaga ggagtatcaa tgcgcatcaa tttgtggtca gtcgaaatca
    13021 tgactttcca gtttggatga tgttgtacaa cttcctgacc aaggtgttcg acgaaagctg
    13081 tccggctaac cgcttttccc gttaaccgat tgaataatga tgtcaaaatg agttccctct
    13141 cttattattc cttgctaaat ggttagttaa ccttcaccag cgtgcgaccc tggatctggt
    13201 tattaatgat ggcctcggca aagttcggtg cctctgacag agatatctct tttgccgcct
    13261 gggtatagaa tgattccggt aaatcggcga ccagtcgctg ccaggcttgt gcgcggcgtt
    13321 ctggtggcgt cattactgaa tccacccctt gcaaacggac attacgcaga ataaatggca
    13381 tgaccgtggt tggcagagta aaaccacccg ccagaccaca ggccgccacg cagccgccgt
    13441 aattcatttg cgccagcact tttgccagca ctttgtcgcc aacagtgtca attgccccag
    13501 cccagacttg tttttccaga ggacgggatt cggcaaactc atcacgaggg agaatacggc
    13561 tggcacctaa acttttcaga tattcatggg tactttcgcg accggaaacg gcaacgacct
    13621 gataacccaa cttatgcagc agcgccacgg cggtactgcc gacgccacca ctggcacccg
    13681 tcacgacaat ctccccgtcc tgcgggcgaa caccggcatc ttccagcgcc atcacacaca
    13741 gcatggcggt aaaaccggca gtaccgataa tcattgcttt acgcgcgtcc agcccttgcg
    13801 gcatggcaac cagccagtca cctttcactc gcgcctgctc cgccagccca ccccagtggt
    13861 tttcaccaac gccccagcca gtgagtaaca cctcctgacc ggcatgaaaa cgcggatctt
    13921 cgctggtgcg tacagttccg gcaaaatcga tcccaggaat catcggaaaa ttacggatga
    13981 tttttccctt accggtaatc gccagcgcat ctttatagtt caggctcgac cagtgaacat
    14041 cgaccgtgac atcgccctcc ggcaggcgac tttcgtccag agtctgtact gatgcgagag
    14101 ttttgccgtc ctgctgttct aaaagtaacg cctgcataag tggtcctcat gtgcatgatg
    14161 gattggaaaa taatttctga agattatact cgctaatgga aaagcaaagc cgatgaagcg
    14221 caataaattg cgagataaat ctgatttgct agtatgcccg cttcctcact atcggagtta
    14281 acacaaggat gagattaacg acgaaatttt cggcctttgt tacgctgctc accgggttaa
    14341 caatttttgt gactttgctg ggctgttcgc taagtttcta caacgcaatt cagtataagt
    14401 ttagtcatcg cgttcaggcg gtggcgacgg cgattgatac ccaccttgtg tcgaatgact
    14461 tcagcacatt aaggccacaa attaccgaat taatgatgtc ggcagatatc gttcgtgtag
    14521 acctgctcca tggtgataaa caggtttata ccctggccag aaatggtagt tatcgtccgg
    14581 ttggctccag cgatctgttt cgcgaactga gtgttccgtt gataaagcat ccggggatgt
    14641 cgctgcgtct ggtttatcag gatccgatgg gcaactattt ccattcttta atgactaccg
    14701 cgccgctcac gggggcgatt ggctttatca ttgttatgct cttcctggcg gtacgctggt
    14761 tacaacggca acttgccggg caagaattgc tggaaacccg ggctactcgt atcttaaacg
    14821 gtgagcgtgg ctctaatgtg ttgggaacca tctatgaatg gccgcccaga accagcagtg
    14881 cgctagatac gctgcttcgt gaaattcaga acgcacgcga acaacacagc cgtcttgata
    14941 cgctgatccg ctcttatgcc gcccaggacg tgaaaaccgg cctcaataac cgactctttt
    15001 tcgataatca gttagcaacg ttactggaag atcaggagaa agtaggtacc cacgggatcg
    15061 tgatgatgat tcgtctgccg gatttcaata tgttgagcga tacttggggg cacagccagg
    15121 ttgaagaaca gttcttcact ctgactaatc tgctgtcgac atttatgatg cgctaccctg
    15181 gcgcactgct ggcgcgttac caccgcagtg attttgctgc gctgttaccg caccggacgt
    15241 taaaagaggc agagagcatc gccggtcagt taatcaaagc cgtcgatacc ttgccgaaca
    15301 ataaaatgct cgatcgcgac gatatgatcc acattggtat ctgcgcctgg cgtagtggtc
    15361 aggataccga gcaggtaatg gaacatgcag agtctgccac gcgtaatgcg ggattgcagg
    15421 gcggcaatag ctgggctatt tacgatgact cgttgcctga aaaaggacgc ggtaatgttc
    15481 gctggcgtac gcttatcgag caaatgctca gtcgcggcgg cccgcgcctt tatcaaaaac
    15541 cggcggttac tcgcgaaggt caggttcatc atcgcgaact catgtgccgc atcttcgatg
    15601 gtaatgaaga ggttagctcg gcggagtata tgccgatggt cttgcagttt ggcttatcgg
    15661 aagagtatga ccgtctgcaa atcagccgtc ttattccact attgcgttac tggccagagg
    15721 aaaatctggc gattcaggtt accgttgagt cgctgattcg cccgcgtttt cagcgttggc
    15781 tgcgcgatac gttaatgcaa tgtgaaaaat cacaacgaaa acgcataatt attgaacttg
    15841 cagaggccga tgtaggtcaa catatcagtc gtttacaacc tgttattcgt ttagtgaatg
    15901 ctttaggggt acgggtagcc gtcaaccagg ctggtttgac gctggtaagc accagttgga
    15961 tcaaagaact taatgttgag ttactcaagc tccatccggg gctggtcaga aacattgaga
    16021 agcgaacgga gaaccagctg ctggttcaaa gcctggtgga agcctgctcc gggaccagca
    16081 cccacgttta cgccaccggc gtgcgttcgc gaagcgagtg gcagaccctg attcagcgcg
    16141 gtgttacagg cgggcaaggg gattttttcg cgtcctcaca gccacttgat actaacgtga
    16201 aaaaatattc acaaagatac tcggtttaac ctgccgttta atccgttttc acgtagaata
    16261 acgcgcgctg cgtctcatgg gagtgtgctt gtctgctcgc cagattgttg cagcacatat
    16321 gcagatgaat gaccttacgc ggttgcaaac aggcgaggaa tgctgctgat gcattaagcc
    16381 tttctggact caggcagaga tttgtaacaa aggaaacgaa ctgcactaat tttcaccgta
    16441 gcagatgatt tttgcgcctt gtcgctgctg cgtgtggttg gtaaagtaag cggattttct
    16501 tttccgcccc agctttcagg attatccctt agtatgttga aaaaatttcg tggcatgttt
    16561 tccaatgact tgtccattga cctgggtact gcgaataccc tcatttatgt aaaaggacaa
    16621 ggcatcgtat tgaatgagcc ttccgtggtg gccattcgtc aggatcgtgc cggttcaccg
    16681 aaaagcgtag ctgcagtagg tcatgacgcg aagcagatgc tgggccgtac gccgggcaat
    16741 attgctgcca ttcgcccaat gaaagacggc gttatcgccg acttcttcgt gactgaaaaa
    16801 atgctccagc acttcatcaa acaagtgcac agcaacagct ttatgcgtcc aagcccgcgc
    16861 gttctggttt gtgtaccggt tggcgcgacc caggttgaac gccgcgcaat tcgtgaatcc
    16921 gcgcagggcg ctggtgcccg tgaagtcttc ctgattgaag aaccgatggc tgccgcaatt
    16981 ggtgctggcc tgccggtttc tgaagcgacc ggttctatgg tggttgatat cggtggtggt
    17041 accactgaag ttgctgttat ctccttgaac ggcgtggttt actcctcttc cgtgcgcatt
    17101 ggtggtgatc gtttcgacga agctatcatc aactatgtgc gtcgtaatta cggttctctg
    17161 atcggtgaag ccaccgcaga acgtatcaag cacgaaatcg gttcggctta cccgggcgat
    17221 gaagtccgtg aaatcgaagt tcgtggccgt aacctggcag aaggtgttcc acgcggtttt
    17281 accctgaact ccaacgaaat cctcgaagca ctgcaggaac cgctgaccgg tattgtgagc
    17341 gcggtaatgg ttgcactgga acagtgcccg ccggaactgg cttccgacat ctccgagcgc
    17401 ggcatggtgc tcaccggtgg tggcgcactg ctgcgtaacc ttgaccgttt gttaatggaa
    17461 gaaaccggca ttccagtagt tgttgctgaa gacccgctga cctgtgtggc gcgcggtggc
    17521 ggcaaagcgc tggaaatgat cgacatgcac ggcggcgacc tgttcagcga agagtaatcg
    17581 gatgcaggca ggggaaatgt ctgtttaccc tgcctggtct gatacgagaa tacgcataac
    17641 ttatgaagcc aatttttagc cgtggcccgt cgctacagat tcgccttatt ctggcggtgc
    17701 tggtggcgct cggcattatt attgccgaca gccgcctggg gacgttcagt caaatccgta
    17761 cttatatgga taccgccgtc agtcctttct actttgtttc caatgctcct cgtgaattgc
    17821 tggatggcgt atcgcagacg ctggcctcgc gtgaccaact agaacttgaa aaccgggcgt
    17881 tacgtcagga actgttgctg aaaaacagtg aactgctgat gcttggacaa tacaaacagg
    17941 agaacgcgcg tctgcgcgag ctgctgggtt ccccgctgcg tcaggatgag cagaaaatgg
    18001 tgactcaggt tatctccacg gttaacgatc cttatagcga tcaagttgtt atcgataaag
    18061 gtagcgttaa tggcgtttat gaaggccagc cggtcatcag cgacaaaggt gttgttggtc
    18121 aggtggtggc cgtcgctaaa ctgaccagtc gcgtgctgct gatttgtgat gcgacccacg
    18181 cgctgccaat ccaggtgctg cgcaacgata tccgcgtaat tgcagccggt aacggttgta
    18241 cggatgattt gcagcttgag catctgccag cgaatacgga tattcgtgtt ggtgatgtgc
    18301 tggtgacctc cggtctgggc ggtcgtttcc cggaaggcta tccggtcgcg gttgtctctt
    18361 ccgtaaaact cgatacccag cgcgcttata ctgtgattca ggcgcgtccg actgcaggac
    18421 tgcaacgttt gcgttatctg ctgctgctgt ggggcgcgga tcgtaacggc gctaacccga
    18481 tgacgccgga agaggtgcat cgtgttgcta atgaacgtct gatgcagatg atgccgcagg
    18541 tattgccttc gccagacgcg atggggccaa agttacctga accggcaacg gggatcgctc
    18601 agccgactcc gcagcaaccg acgacaggaa atgcagctac tgcgcctgct gcgcccacac
    18661 agcctgctgc taatcgctct ccacaaaggg ctacgccgcc gcaaagtggt gctcaaccgc
    18721 ctgcgcgtgc gccgggaggg caatagtggc gagctatcgt agccagggac gttgggtaat
    18781 ctggctctct ttcctcattg cgctgttgct gcaaatcatg ccctggccgg ataacctgat
    18841 tgttttccgg ccaaactggg tgttactcat cttgttgtat tggatcctgg ccttgcctca
    18901 tcgcgtaaat gtgggcacag gttttgtgat gggtgccata ctggatctga tcagcggctc
    18961 gacgcttggc gtacgcgtat tggcgatgag catcattgct tacctggtgg cgctgaaata
    19021 ccagcttttc cgcaacctcg cattatggca gcaggcgctg gtcgtcatgt tgctttcgct
    19081 ggtggtggat attattgttt tctgggcaga gtttttagtg attaacgtct ctttcagacc
    19141 ggaagtgttc tggagtagtg tagtcaatgg ggtgctctgg ccgtggattt tcttgctgat
    19201 gcgtaaagtc cgtcagcagt ttgcagtgca ataaaggttt ctatgacttc tctgtattta
    19261 gcttccggtt ctccgcgtcg tcaggagtta cttgcgcaac ttggtgtgac ctttgaacgt
    19321 attgttacgg gcattgagga gcagcgtcag ccgcaggaga gcgcgcagca gtatgttgtg
    19381 cgtctggcgc gcgagaaagc acaggcgggt gtcgcgcaaa cggcgcagga tctcccggtg
    19441 ctgggtgcgg atactatcgt tatcctgaat ggagaagtgc tggagaaacc gcgcgacgca
    19501 gagcatgcgg cgcagatgtt gcgcaaatta tcgggtcaga cccatcaggt gatgacggca
    19561 gtggcgttgg ccgacagcca gcacattctc gattgcctgg tggtcaccga tgtgactttc
    19621 agaacgttaa cagacgaaga catcgcgggc tatgtcgcca gcggtgaacc gttagataaa
    19681 gcaggtgcat acggtattca ggggctgggt ggctgttttg tcaggaagat aaatggcagc
    19741 tatcacgccg tagtcggctt acctctggtt gaaacgtatg aattattaag taattttaac
    19801 gcactgcgtg agaaaaggga taaacatgac ggctgaattg ttagtaaacg taacgccttc
    19861 ggaaacgcga gtggcgtata ttgatggcgg tattctgcag gaaattcata ttgaacgtga
    19921 ggcgcgacgc ggaatagtag gcaatatcta caagggtcgt gtaagtcgtg tacttccggg
    19981 tatgcaggcg gcttttgtag atattgggct ggataaagcc gcgtttcttc atgcatccga
    20041 tatcatgccg cacaccgaat gtgtggcggg tgaagaacaa aagcaattca cggtgcgcga
    20101 catctcggaa ctggttcgtc aggggcaaga tctgatggtg caggtggtga aagatccgct
    20161 tggcactaaa ggtgcgcgcc tgaccaccga tatcacgctg ccttctcgct atctggtctt
    20221 tatgccaggg gcttctcacg ttggggtttc ccaacgtatt gaaagcgaat cagaacgtga
    20281 acgcctgaaa aaagtggtcg cagagtattg cgacgagcag ggcgggttta tcatccgtac
    20341 cgcagcggaa ggggttggcg aggctgaact ggcctccgat gccgcttatc tgaaacgcgt
    20401 ctggaccaaa gttatggagc gtaaaaagcg cccgcagacc cgttatcagc tgtacggcga
    20461 actggcgctg gctcagcgtg ttctgcgtga tttcgccgac gccgaactgg accgcattcg
    20521 cgttgactca cgcctgactt acgaagcatt acttgagttc acctcggagt acattcccga
    20581 gatgaccagc aagctggagc attacaccgg acgccagccg attttcgatc tctttgatgt
    20641 cgaaaacgaa atccagcgag cgctggaacg caaagtagaa ctgaaatccg gtggttatct
    20701 cattatcgac cagaccgaag cgatgaccac cgtggacatc aataccggag cgtttgtcgg
    20761 tcatcgcaat ctggacgaca ccattttcaa taccaatatt gaagcgacgc aggctatcgc
    20821 tcgccagtta cggttgcgta atctgggcgg gattatcatt attgatttca tcgatatgaa
    20881 taatgaagat caccgccgcc gcgtgctgca ctcgctggag caggcgttga gcaaagaccg
    20941 ggtgaaaacc agcgttaatg gtttttcggc gctggggctg gtggagatga cgcgtaaacg
    21001 cacccgcgaa agcattgagc acgtactgtg taacgaatgc ccaacctgcc acggtcgcgg
    21061 aacggtgaaa accgtggaaa cggtatgcta tgaaatcatg cgcgagattg ttcgtgtcca
    21121 ccatgcttac gactccgacc gtttcctggt ctatgcttct ccggcagtag ccgaagcctt
    21181 gaaaggcgaa gagtcacact cgctggcgga agtggaaatt ttcgttggca aacaggttaa
    21241 agtacaaatt gaaccgctct ataaccagga gcagtttgac gtcgtaatga tgtaaacaga
    21301 tgctgggccg ccatccggca aagggttttt gagtcacatt tttagcagac aaggagtgac
    21361 gggtgaggcg attgccgggg attttactgc ttactggagc cgcgctcgtt gtgatcgctg
    21421 ccctgctggt tagcggcctg cgtattgctt taccgcatct tgacgcctgg cgtccggaaa
    21481 tcctcaacaa aatagaatcc gcgactggca tgccggtaga agccagtcag ctctcagcca
    21541 gctggcagaa ttttggcccg acgcttgaag cacacgacat ccgtgcagaa ctaaaagatg
    21601 gcggcgaatt ttcggttaaa cgcgttactc tggcgctgga tgtctggcag agcctgttac
    21661 atatgcgctg gcagtttcgc gacctcactt tctggcagct gcgctttcgc accaacactc
    21721 ctatcaccag cggtggtagc gatgacagtc tggaagccag tcacatcagc gatctgtttc
    21781 ttcgtcaatt tgaccatttc gatctccgcg acagtgaagt cagtttcctg acgccatccg
    21841 gtcagcgtgc cgagctggcg atcccacaac tcacctggct gaacgatcca cgtcgacacc
    21901 gtgcggaagg cctggtaagc ctctccagcc ttaccggaca gcacggcgtg atgcaggtgc
    21961 gcatggattt gcgcgatgat gaggggttgt taagcaatgg tcgcgtctgg ctacaggcgg
    22021 atgacatcga cctgaagccg tggctcggta aatggatgca ggacaatatt gctctggaaa
    22081 cggcacagtt ctcccttgaa ggctggatga cgatcgacaa aggcgatgta accggcggtg
    22141 acgtctggct gaaacagggc ggtgccagct ggttgggcga gaagcaaacg catacgctgt
    22201 cggtggataa tctgaccgcg catattacgc gtgaaaatcc gggctggcag ttctctattc
    22261 ccgatacacg gatcacgatg gacggcaaac cctggccgag cggagcattg acgctggcct
    22321 ggataccgga acaggacgtt ggcggcaaag acaataaacg cagtgacgaa ctccggattc
    22381 gcgccagtaa tctggagctg gcaggcctgg agggcatacg cccgctggcc gcgaaacttt
    22441 cacctgcact gggtgatgtt tggcgctcca cacaaccgag cggcaagatt aacactctgg
    22501 cgctggatat cccgcttcag gcggcagaca agacccgttt tcaggcatcg tggagcgatc
    22561 tggcctggaa gcaatggaaa ttattaccgg gtgcggaaca cttctccggg acgctttccg
    22621 gcagcgttga aaatggtttg cttaccgcgt cgatgaagca ggcaaagatg ccttacgaaa
    22681 cggtattccg tgcgccactg gaaatcgccg acggccaggc aactataagc tggctgaaca
    22741 atgacaaagg tttccagctg gatgggcgta atattgacgt taaagccaaa gccgtccatg
    22801 cgcgcggcgg ttttcgttac ctgcaacctg ctaacgatga accctggctg ggtattctgg
    22861 ctggcatcag taccgatgat ggttcacaag cctggcacta tttcccggaa aacttgatgg
    22921 gtaaagacct ggttgattac ttaagtggcg cgattcaggg cggtgaagcg gataacgcga
    22981 cgctggttta tggtggcaat ccgcaactct tcccctataa acacaacgaa ggtcagtttg
    23041 aagtgctggt gccgctgcgc aacgcgaagt ttgccttcca gccggactgg cctgcattaa
    23101 ctaaccttga tattgaactg gactttatta acgacggttt atggatgaaa accgatggcg
    23161 ttaatctggg cggcgtgcgc gcgagtaatc tcaccgcagt gatccctgac tactcgaaag
    23221 aaaaactgct gattgacgct gacattaaag gtccgggtaa agccgttggc ccttactttg
    23281 atgagacacc gctgaaagat tctctgggtg cgaccctgca agaactccag ctcgacggcg
    23341 atgtgaatgc tcgcttacat cttgatatcc cgctgaacgg cgagctggta accgcgaaag
    23401 gtgaagtgac gctgcgtaat aacagtctgt ttatcaaacc actcgacagc accctgaaaa
    23461 atttgagcgg taaattcagc tttatcaatg gcgatctgca aagtgaacca ctgacagcaa
    23521 gctggtttaa tcagccgttg aacgtggatt tttccaccaa agaaggggca aaagcctacc
    23581 aggttgcggt gaatctcaac ggtaactggc aaccggcgaa aaccggcgtt ctgcctgaag
    23641 cggtgaacga agcattgagt ggcagcgtgg cgtgggatgg taaagtgggc attgatctgc
    23701 cttatcatgc tggcgcgacc tataacgtag agctgaacgg cgatctgaat aatgtgagta
    23761 gtcacttacc ttcaccgtta gccaaacctg cgggtgaacc actagcggta aacgttaagg
    23821 ttgatggcaa tctcaacagc tttgaattaa ccggacaggc tggtgcggat aaccatttca
    23881 atagccgctg gttgcttggt caaaagctga cgctcgatcg tgctatttgg gcggcagaca
    23941 gtaaaacgct cccgccgttg ccggaacaaa gtggcgttga actcaatatg ccgccgatga
    24001 atggtgccga gtggctggcc ctgttccaga aaggcgctgc ggagagtgtc ggtggtgcag
    24061 cgagtttccc acaatacata acgttacgta cgcctatgtt gtcactggga aatcagcaat
    24121 ggaataacct gagtattgtt tcgcaaccga cggcaaatgg caccctggtt gaggcgcaag
    24181 ggcgtgaaat caacgccacg ctagcgatgc gtaataacgc gccgtggctg gcgaatatca
    24241 aatatcttta ttacaacccg agcgtggcga aaactcgtgg tgattcaacg ccgtcatcac
    24301 ctttcccgac aacggagcgc attaacttcc gtggctggcc ggacgcacaa atacgatgca
    24361 cagagtgctg gttctggggg caaaaattcg gtcgcattga cagtgatatc accatttctg
    24421 gcgatacgtt aacgctgacc aatggactga ttgatactgg tttctcgcgg cttactgccg
    24481 atggtgaatg ggttaataat ccggggaatg aacgtacctc gctgaaagga aaactgcgcg
    24541 ggcagaaaat tgatgccgcc gcagaatttt ttggtgtcac gacgcccata cgccagtcgt
    24601 catttaatgt ggattacgat ttacactggc gcaaagcacc ctggcagcca gatgaagcga
    24661 cgttgaatgg catcattcat actcaactgg gtaaaggcga aattaccgaa atcaataccg
    24721 gacatgccgg gcaattgctg cgcttattga gcgtagatgc cctgatgcgt aagctgcgtt
    24781 ttgatttcag agacactttt ggcgaagggt tctattttga ctccattcgc agcaccgcgt
    24841 ggattaaaga cggggttatg cacaccgacg acacgctggt ggatggcctg gaggcggata
    24901 tcgccatgaa agggtcggta aatctggtac gtcgcgacct gaatatggaa gcggttgtcg
    24961 caccagagat ttctgcgacg gtgggcgtgg ctgcggcttt tgcggttaac cccattgttg
    25021 gcgcggcagt ttttgccgct agtaaagtgc tggggccgct gtggagcaaa gtctccattt
    25081 tgcgctatca catttcgggt ccgctggacg atccgcaaat caacgaagtg ttgcgccaac
    25141 cgcgtaaaga aaaagcgcaa tgatttgacg agggcgcgta attgccccaa tctcatatga
    25201 taatcgttgc caaaggccaa cgagccagaa cataaccgta ggccggatag ggcgttcatg
    25261 ccgcatccgg cagccgtaaa aaatcctcta ctgcagtaac taacgagtag caaaaacgat
    25321 gagtctgaac ctggtaagtg aacaattgct agcggcgaac agcctgaaac atcaggactt
    25381 gttcgcgatc ctcggtcaac tggccgaacg tcgccttgat tatggcgatc tctattttca
    25441 gtcgagctat cacgaatcct gggttttaga agaccgcatt attaaagatg gttcttacaa
    25501 catcgatcag ggcgttggcg tgcgtgcaat cagcggtgaa aaaaccggat ttgcttacgc
    25561 tgaccaaatc agcctgctgg cgctggaaca gagtgcgcaa gcggcgcgca ccatcgtccg
    25621 tgatagtggt gatggcaaag tacagacgct gggcgcggta gagcatagcc cgttgtatac
    25681 ctcggtagat ccgctgcaaa gcatgagccg tgaagagaag ctggatatcc tgcgtcgcgt
    25741 cgataaggtt gctcgcgaag cggacaagcg cgtacaggaa gtgactgcca gcctcagtgg
    25801 tgtctatgaa ttaattctgg ttgcggccac cgacggcacg ctagcggcgg atgtccgtcc
    25861 gctggtgcgt ctttccgtga gcgttctggt cgaagaagat ggcaaacgcg aacgcggtgc
    25921 cagtggcggc ggcggtcgtt ttggttatga gttcttcctt gccgatcttg acggcgaagt
    25981 ccgtgcggat gcatgggcaa aagaagcagt gcgtatggcg ctggtcaatc tttctgccgt
    26041 tgctgcacca gcgggcacca tgccggtagt acttggcgca ggttggccgg gcgtgctgtt
    26101 gcatgaagcg gtaggtcacg gtctggaagg cgacttcaac cgccgcggca cttcagtatt
    26161 tagtgggcag gtcggggagc tggtggcttc agaactgtgt accgtggttg atgacggcac
    26221 gatggtcgat cgccgtggtt cggtggcgat tgatgacgaa ggtacgccag gccagtacaa
    26281 cgtgctgatt gagaacggca ttctgaaagg ctacatgcag gataaactca acgcgcgttt
    26341 gatggggatg acgccgactg gcaacggtcg ccgtgaatcc tacgcccatc tgcccatgcc
    26401 gcgtatgact aacacctata tgctgccggg taaatcgacc ccgcaggaaa ttattgaatc
    26461 cgttgagtac ggtatctatg caccgaactt tggtggcggt caggtggata tcacctccgg
    26521 caaattcgtt ttctccactt cagaagcata tctgattgaa aacggtaaag taacgaagcc
    26581 ggtgaaaggc gcaacgttga ttggttccgg tatcgaaacc atgcagcaga tttcgatggt
    26641 tggcaacgac ctgaaactgg ataacggcgt gggtgtctgc ggtaaagaag ggcaaagttt
    26701 gccggttggc gtgggccagc caacgctgaa agttgataac ctgactgttg gcggtactgc
    26761 gtaataattt cattattttc agaaggataa ttaatctttc tacgtgcacg aacggtcccc
    26821 tcgctcctct ggggttaggg tgaggggaac ccgttggcac aggtttgtag aacgtaacag
    26881 tacaatatga attacttctc tttcccgcgc ccgtgcatct cctgaaacaa tttaccgacc
    26941 tcaacaaaat aatccgtcag cgagttgatc acgacctgta ccttcagcgg cagcttatct
    27001 ttttcggtat ataacgcata aaccgggcgt ggatctgact gataacgcgg cagcaggatc
    27061 tccagctcac cgcgattgat ctcgttgatc acccacatca gcggcacgta ggcgatcccg
    27121 gcacccgccg tcagccagcg caccagcgtc atcggatcat tagtcacaaa tcttccttgt
    27181 gggatcaggc gagtcgagat cccttccggt gcgatcagtt caaattcatt gtcgggccgc
    27241 acgctgtatt caagccatga atgactactc aaatcggcgg gtttttccgg tatgccgtat
    27301 tgtgtgagat agcttttcgc ggcgcacacc accatcggca tcgcgcccag acggcgggaa
    27361 aacaggctgg aattccggta accagattga cgctcaaacc tgggtattct ttcagcattt
    27421 ttgctgtcag cccggcgaga acattttgtg ccatagttga agaacagcca atgcgcagcg
    27481 tcccgatggg ggtgttattg aaggcataca gttgctcatg aacatcctgc acttcatgaa
    27541 gcatccgacg gcagccctgg tagtaaattc tgccggcttc ggtcaggcca atgctgcgtg
    27601 tgctacggtt taacagcttt acctgcaact catcttccag ttttgacacc gtctgactga
    27661 tggacgaaac gctcatctgt agctgtctgg cggcggcggt aaaagagcca aattcaacta
    27721 ctttggcaaa caccgacatg cgttttagtc gttccattat tcactctgac ttaaaagtga
    27781 tttagatcac ataatataga taacagcata acagttacgc taatatatta aatatcaatc
    27841 tacagcaatg ttgctctcgc ccggctttcc atgcccttct ctgcggcgac agatgctgaa
    27901 aataataacg cctgctctct ctttacaacc aaggtcaaca tgagtctgtt tcccgttatc
    27961 gtggtgtttg gactgtcctt cccaccgata ttttttgaat tgcttttatc actggcgatt
    28021 ttctggctgg tgcgccgggt acttgtgcca acaggtatct acgactttgt ctggcatccg
    28081 gcgttgttca acaccgcgct ctattgctgc ttgttttatt tgatatcgcg actgttcgtt
    28141 tgaggttgaa gtgaaaacac taataagaaa attctcccgt acggccatca cggtcgtatt
    28201 agtcattcta gccttcatcg caatttttaa tgcctgggtc tattacaccg aatccccctg
    28261 gacgcgtgac gcgcgcttta gcgctgacgt cgttgcgatc gcgccggacg tttctggact
    28321 cattacccag gtgaatgttc atgataacca gctggtgaaa aaaggacaga tactgttcac
    28381 catcgaccag ccgcgctatc aaaaggcgct tgaggaagcg caagccgatg ttgcttatta
    28441 tcaggtactg gcacaggaga aacgccagga ggccggacgt cgtaaccgtc tcggtgtgca
    28501 ggcgatgtct cgcgaagaga tcgaccaggc caacaacgta ctacaaacgg ttctgcatca
    28561 gttagcgaaa gcgcaggcga cccgcgatct ggcaaaactg gatcttgaac gcacggtgat
    28621 ccgcgcgcca gcagatggct gggtgaccaa cctcaacgtc tataccggtg agtttattac
    28681 tcgaggatca acggcggttg cgctggtgaa acagaactcc ttctatgtac tggcctatat
    28741 ggaagaaact aagctggaag gggggcgtcc ggggtatcgt gcagagatca cgccgcttgg
    28801 cagtaacaaa gtgctgaaag ggactgttga tagtgttgcc gcaggggtca ccaacgccag
    28861 cagcacgcgt gacgacaaag ggatggcgac tatagactct aatcttgaat gggtgcgtct
    28921 tgcgcaacgt gttccggttc gtattcgtct cgacaaccag caagagaaca tctggcctgc
    28981 gggcaccact gctacagtgg tggtcactgg caaacaagat cgcgacgaaa gccaggattc
    29041 gttcttccgt aaaatggccc atcgcctgcg tgagtttggt taatcacgat gggtattttc
    29101 tccattgcta accaacatat tcgctttgcg gtaaaactgg cgaccgccat tgtactggcg
    29161 ctgtttgttg gctttcactt ccagctggaa acgccacgct gggcggtact gacagcggcg
    29221 attgttgccg ccggtccggc ctttgctgcg ggaggtgaac cgtattctgg cgctattcgc
    29281 tatcgtggct ttttgcgcat catcggcaca tttattggct gtattgccgg actggtgatc
    29341 atcattgcga tgatccgcgc accattattg atgattctgg tgtgctgtat ctgggccggt
    29401 ttttgtacct ggatatcctc gctggtacga atagaaaact cgtacgcgtg ggggctggcc
    29461 ggttataccg cgctgatcat tgtgatcacc attcagccgg aaccattgct tacgccgcag
    29521 tttgccgtcg aacgttgtag cgagatcgtt atcggtattg tgtgtgcgat tatggcggat
    29581 ttgctctttt ctccgcgatc gatcaaacaa gaagtagatc gagagctgga aagtttgctg
    29641 gtcgcgcaat atcaattaat gcaactctgt atcaagcatg gcgatggtga agttgtcgat
    29701 aaagcctggg gcgacctggt gcgacgcacc acggcactac aaggcatgcg cagcaacctg
    29761 aatatggaat cttcccgctg ggcgcgggcc aatcgacgtt taaaagcgat caatacgcta
    29821 tcgctgacgc tgattaccca atcctgcgaa acttatctta ttcagaatac gcgcccggaa
    29881 ttgatcactg atactttccg cgaatttttt gacacgccgg tagaaaccgc gcaggacgtc
    29941 cacaagcagc tcaaacgcct gcggagagtt atcgcctgga ccggggaacg ggaaacgcct
    30001 gtcaccattt atagctgggt cgcggcggca acgcgttatc agcttctcaa gcgcggcgtt
    30061 atcagtaaca caaaaatcaa cgccaccgaa gaagagatcc tgcaaggcga accggaagta
    30121 aaagtagagt cagccgaacg tcatcatgca atggttaact tctggcgaac cacactttcc
    30181 tgcattctgg gcacgctttt ctggctgtgg acgggctgga cttccggcag tggtgcaatg
    30241 gtgatgattg cggtcgtgac gtcactggca atgcgtttgc cgaatccacg catggtggcg
    30301 atcgacttta tctatgggac gctggccgcg ctgccgttag ggctgctcta ctttttggtg
    30361 attatcccta atacccaaca gagcatgttg ctgctgtgta ttagcctggc agtgctggga
    30421 ttcttcctcg gtatagaagt acagaaacgg cgactgggct cgatgggggc actggccagc
    30481 accataaata ttatcgtgct ggataacccg atgactttcc atttcagtca gtttctcgac
    30541 agcgcattag ggcaaatcgt cggctgtgtg ctcgcgttca ccgttatttt gctggtgcgg
    30601 gataaatcgc gcgacaggac tggacgtgta ctgcttaatc agtttgtttt tgccgctgtt
    30661 tccgcgatga ctaccaatgt ggcacgtcgt aaagagaacc acctcccggc actttatcag
    30721 cagctgtttt tgctgatgaa taagttccca ggggatttgc cgaaatttcg cctggcgctg
    30781 acgatgatta tcgcgcacca gcgcctgcgt gatgcgccga tcccggttaa cgaggattta
    30841 tcggcgtttc accgacaaat gcgccgcaca gcagaccatg tgatatctgc ccgtagcgat
    30901 gataaacgtc gtcggtactt tggccagttg ctggaagaac tggaaatcta ccaggaaaag
    30961 ctacgcatct ggcaagcgcc accgcaggtg acggaaccgg ttcatcggct ggcggggatg
    31021 ctccataagt atcaacatgc gttgaccgat agttaagtca aaaccgacgc caaaagcgtc
    31081 ggttttttca tggctatact tagcgatgca cggcagaact cgccgcgaaa cgtgacggtg
    31141 gcaacagatg aatatttata cctttgattt tgatgagatt gagagtcagg aggattttta
    31201 tcgtgacttt agccaaacct ttggtctggc taaagataag gtacgcgatc tcgactcact
    31261 atgggatgtg ttaatgaacg atgtcctgcc gctaccactt gagattgaat ttgttcatct
    31321 gggagagaaa acgcgtcgcc gttttggcgc gttaatattg ctgtttgatg aggcagagga
    31381 agagctggaa gggcatttgc gttttaatgt tcgtcattag cgcaaaaaaa agcccccgaa
    31441 ccgggggcaa tatcgtcgga caagacgatg agggtttatt tgtacagttc agccgtagcg
    31501 tgccaggtgt caccgctacg agcttcagta atctggtagg ccgttgcgcc tttctcttcc
    31561 gcttttttgt tcagcatttc acgcatatcc attggcgaag acgccacacc acttacggat
    31621 acggtcccga ttgcttcacg attttgtgct tgtgcagtat caatggagtc ggcagcgaat
    31681 gcaccgaaag agagaacaga aagtacgctt aatgcagcta cagtggtttt gattttcatg
    31741 atttttacct cgacataatc ttttagctgg gcctttgttt cgtgatccat atcacaaaat
    31801 caagcataca ctaattattg aactaattaa tacca
//
LOCUS       DAEEKQ010000028        31794 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.028, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000028 DAEEKQ010000000
VERSION     DAEEKQ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.028"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..1133
                     /gene="ypdA"
                     /locus_tag="KLK57_001318"
     CDS             <1..1133
                     /gene="ypdA"
                     /locus_tag="KLK57_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBE0891738.1"
                     /translation="LVQSVEGEKEASAARQTKLALDIANKTLPLFRHVNSESLRKVCE
                     IIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEA
                     HRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRA
                     EQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIE
                     LKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAI
                     VHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNV
                     HHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            1148..1882
                     /gene="ypdB"
                     /locus_tag="KLK57_001319"
     CDS             1148..1882
                     /gene="ypdB"
                     /locus_tag="KLK57_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HBE0891739.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            1895..2752
                     /locus_tag="KLK57_001320"
     CDS             1895..2752
                     /locus_tag="KLK57_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0891740.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(2755..5250)
                     /gene="ptsP"
                     /locus_tag="KLK57_001321"
                     /pseudo
     CDS             complement(2755..5250)
                     /gene="ptsP"
                     /locus_tag="KLK57_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
     gene            complement(5275..6312)
                     /gene="ypdE"
                     /locus_tag="KLK57_001322"
     CDS             complement(5275..6312)
                     /gene="ypdE"
                     /locus_tag="KLK57_001322"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HBE0891741.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTAFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVSAEIGVPLQAD
                     MFSNGGTDGGAVHLTDTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(6312..7397)
                     /gene="ypdF"
                     /locus_tag="KLK57_001323"
                     /pseudo
     CDS             complement(6312..7397)
                     /gene="ypdF"
                     /locus_tag="KLK57_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(7412..8659)
                     /locus_tag="KLK57_001324"
     CDS             complement(7412..8659)
                     /locus_tag="KLK57_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HBE0891742.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(8681..9007)
                     /locus_tag="KLK57_001325"
     CDS             complement(8681..9007)
                     /locus_tag="KLK57_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HBE0891743.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(9227..10192)
                     /gene="glk"
                     /locus_tag="KLK57_001326"
     CDS             complement(9227..10192)
                     /gene="glk"
                     /locus_tag="KLK57_001326"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HBE0891744.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALTLGTFGGVYIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            10396..11652
                     /locus_tag="KLK57_001327"
     CDS             10396..11652
                     /locus_tag="KLK57_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HBE0891745.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPIWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            11767..12093
                     /locus_tag="KLK57_001328"
     CDS             11767..12093
                     /locus_tag="KLK57_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBE0891746.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(12234..13472)
                     /locus_tag="KLK57_001329"
     CDS             complement(12234..13472)
                     /locus_tag="KLK57_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HBE0891747.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            13808..15010
                     /gene="nupC"
                     /locus_tag="KLK57_001330"
     CDS             13808..15010
                     /gene="nupC"
                     /locus_tag="KLK57_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HBE0891748.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLRFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(15060..17248)
                     /locus_tag="KLK57_001331"
                     /pseudo
     CDS             complement(15060..17248)
                     /locus_tag="KLK57_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311300.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(17457..17532)
                     /locus_tag="KLK57_001332"
     tRNA            complement(17457..17532)
                     /locus_tag="KLK57_001332"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17497..17499),aa:Ala,seq:ggc)
     gene            complement(17572..17647)
                     /locus_tag="KLK57_001333"
     tRNA            complement(17572..17647)
                     /locus_tag="KLK57_001333"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17612..17614),aa:Ala,seq:ggc)
     gene            17868..18227
                     /locus_tag="KLK57_001334"
     CDS             17868..18227
                     /locus_tag="KLK57_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBE0891749.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            18229..18621
                     /locus_tag="KLK57_001335"
     CDS             18229..18621
                     /locus_tag="KLK57_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HBE0891750.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQGKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(18673..20088)
                     /gene="gltX"
                     /locus_tag="KLK57_001336"
     CDS             complement(18673..20088)
                     /gene="gltX"
                     /locus_tag="KLK57_001336"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HBE0891751.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            20347..20422
                     /locus_tag="KLK57_001337"
     tRNA            20347..20422
                     /locus_tag="KLK57_001337"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20380..20382,aa:Val,seq:tac)
     gene            20468..20543
                     /locus_tag="KLK57_001338"
     tRNA            20468..20543
                     /locus_tag="KLK57_001338"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20501..20503,aa:Val,seq:tac)
     gene            20590..20665
                     /locus_tag="KLK57_001339"
     tRNA            20590..20665
                     /locus_tag="KLK57_001339"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20623..20625,aa:Val,seq:tac)
     gene            20670..20745
                     /locus_tag="KLK57_001340"
     tRNA            20670..20745
                     /locus_tag="KLK57_001340"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20703..20705,aa:Lys,seq:ttt)
     gene            20776..20901
                     /locus_tag="KLK57_001341"
     ncRNA           20776..20901
                     /ncRNA_class="other"
                     /locus_tag="KLK57_001341"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            21021..21362
                     /locus_tag="KLK57_001342"
     CDS             21021..21362
                     /locus_tag="KLK57_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HBE0891752.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASKIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQRQQAEKAQEKEQRLSLNVSLLNP
                     VENTTHIDIYI"
     gene            complement(21353..22279)
                     /locus_tag="KLK57_001343"
     CDS             complement(21353..22279)
                     /locus_tag="KLK57_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0891753.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFCIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGAPLYQI"
     gene            22369..23367
                     /locus_tag="KLK57_001344"
     CDS             22369..23367
                     /locus_tag="KLK57_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HBE0891754.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(23364..23582)
                     /locus_tag="KLK57_001345"
     CDS             complement(23364..23582)
                     /locus_tag="KLK57_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HBE0891755.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(23584..25599)
                     /gene="ligA"
                     /locus_tag="KLK57_001346"
     CDS             complement(23584..25599)
                     /gene="ligA"
                     /locus_tag="KLK57_001346"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HBE0891756.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEVLEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(25670..26656)
                     /gene="zipA"
                     /locus_tag="KLK57_001347"
     CDS             complement(25670..26656)
                     /gene="zipA"
                     /locus_tag="KLK57_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HBE0891757.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPIQQPPEAQVPPQHAPRPAQPMQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            26886..27647
                     /gene="cysZ"
                     /locus_tag="KLK57_001348"
     CDS             26886..27647
                     /gene="cysZ"
                     /locus_tag="KLK57_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HBE0891758.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            27832..28803
                     /gene="cysK"
                     /locus_tag="KLK57_001349"
     CDS             27832..28803
                     /gene="cysK"
                     /locus_tag="KLK57_001349"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HBE0891759.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            29187..29444
                     /gene="ptsH"
                     /locus_tag="KLK57_001350"
     CDS             29187..29444
                     /gene="ptsH"
                     /locus_tag="KLK57_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HBE0891760.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            29489..31216
                     /gene="ptsI"
                     /locus_tag="KLK57_001351"
     CDS             29489..31216
                     /gene="ptsI"
                     /locus_tag="KLK57_001351"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HBE0891761.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            31257..31766
                     /gene="crr"
                     /locus_tag="KLK57_001352"
     CDS             31257..31766
                     /gene="crr"
                     /locus_tag="KLK57_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HBE0891762.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
BASE COUNT         7843 a         8383 c         7906 g         7662 t
ORIGIN      
        1 ttctggtgca aagcgttgag ggcgaaaaag aggccagcgc cgcgcggcag accaagctgg
       61 cgctggatat cgccaacaaa acgctaccgc tgtttcgcca tgtcaatagc gagtcattac
      121 gcaaggtctg cgaaattatc cgcgatgaca ttcacgccga tgcggtggcg attactaata
      181 ccgatcatgt gctggcctat gttggcgtgg gtgaacataa ctatcagaat ggcgatgact
      241 tcattagccc gactacccgt caggcgatga attacggaaa aatcatcatt aaaaacaatg
      301 atgaagccca ccgcacacca gagattcatt ccatgctggt gatcccattg tgggagaaag
      361 gggtcgtgac cggaacgctg aaaatttact actgccacgc gcatcagatc acctcgtcat
      421 tacaggaaat ggcggtcggt ctgtcgcaaa tcatctccac gcaactggag gtttcacgcg
      481 ccgagcagct acgtgaaatg gcaaataagg cagagcttcg cgccctgcaa agcaaaatta
      541 atccccattt tctgtttaac gctctgaacg ctatttcatc gtcaatccgt ctgaatccgg
      601 ataccgctcg ccagttgatc tttaatctgt cgcgttatct gcgctataac attgaattaa
      661 aagacgatga gcaaatcgat atcaaaaaag agctgtatca aattaaagac tatattgcca
      721 ttgagcaggc ccgctttggt gacaagctga cggttatcta tgatattgat gaagaggtga
      781 attgctgcat tcccagcctg ctgatccagc cgttggtgga gaacgccatt gtccacggta
      841 ttcagccttg caaaggtaaa ggcgttgtca ccatcagcgt tgcagagtgc ggaaatcggg
      901 tacgcattgc ggtgcgagat accgggcacg gcatcgatcc aaaggtgatt gagcgggtcg
      961 aagcgaatga aatgccgggc aataaaattg gcctgctgaa tgtccatcat cgcgtgaagt
     1021 tattgtatgg cgaggggctg catatccgcc gcctggagcc ggggacggaa attgcgtttt
     1081 acattcctaa ccaacgcacc ccagtcgcct cacaggctac gttattgctt tgagccggag
     1141 tgatattgtg aaagtcatca ttgttgaaga cgaattcctg gcacaacagg aactgagctg
     1201 gctaattaaa gagcacagcc agatggagat tgtcggcacc tttgacgacg gtctggacgt
     1261 gttgaagttt ttgcagcata accgcgtcga cgccattttt ctggatatca atattccgtc
     1321 gctggatggc gtgttgctgg cgcaaaacat cagccagttc gcccataaac cgtttattgt
     1381 gttcatcacc gcgtggaaag aacatgcggt agaagcgttt gaactggagg cgtttgacta
     1441 cattctcaaa ccgtatcagg agtcacgtat taccgggatg ctgcaaaaac tggaagcggc
     1501 ctggcaacaa cagcagacca gcagtacgcc tgccgcgacg gtaacgcgtg agaatgacac
     1561 cattaatctg gtgaaagatg agcgaataat cgtcacgcca attaacgata tctattacgc
     1621 cgaagcgcac gagaaaatga cctttgtcta tacgcggcgt gaatcctacg taatgccgat
     1681 gaacattacc gaattttgca gcaaactgcc gccgtcgcat tttttccgct gccatcgctc
     1741 attttgtgtc aatctgaaca aaatacgcga aatcgaaccg tggtttaata acacctacat
     1801 tctgcgactg aaagatctgg attttgaagt gccggtcagc cgcagcaaag tgaaagaatt
     1861 tcgccagtta atgcatcttt aatcgggaac tttcatgaaa gcaccaggct tgcccgccga
     1921 tcaacaattt ttcgccgatc tgttcagcgg cctggtgctt aacccgcaac tactggggcg
     1981 cgtctggttt gccagccagc ctgcctcgtt accggtgggc agtttatgta ttgattttcc
     2041 ccgtctggat atcgtgctgc gcggcgaata cggcaacctg ctggaagcaa agcagcagcg
     2101 aatggtggaa ggagaaatgc tgtttattcc ggcacgcgcg gctaatttac cgatcaacaa
     2161 caaaccggtg atgctgttaa gcctggtgtt cgcaccgacc tggcttgggt tatcgtttta
     2221 cgatagccgc accacgtcgt tgttgcatcc tgctcgccag atccagcttc ccagcctgca
     2281 acgcggtgaa ggtgaagcga tgcttaccgc cctcacccat cttagccgtt cgccgctgga
     2341 gcagaatatc attcagccac tggtgttaag tttgctgcat ctttgccgca acgtggtgaa
     2401 tatgccgccg ggtaattcgc aaccgcgcgg cgattttctc tatcacagca tttgtaactg
     2461 ggttcaggat aattatgccc agccgctcac ccgcgagagc gtggcgcagt tttttaatat
     2521 cacgcccaat catctgtcaa aactgtttgc tcagcatgga acgatgcgtt ttatcgagta
     2581 tgtgcgttgg gtgcgaatgg cgaaggcgag gatgattttg cagaaatatc atctgtcaat
     2641 tcatgaagtg gcacagcgtt gcggttttcc ggatagcgac tatttttgtc gcgttttccg
     2701 gcgtcagttt ggtctgacgc cgggagagta cagcgcccgt tttcagggct aacgtcagaa
     2761 ggttaattct gtttccagca gcgtcaggat actttgtgca tcttgcgcgg caaacaacga
     2821 ctggcggaag tttttattca ccagtttacg cgccagctgc gagaagactt tcacatgatt
     2881 catcccttcg ttagcaccca gcgtcagcat gatcaccagt tcaacttcgc ccatttctga
     2941 ctgccagtca accggtttcg ccagccgggc aatgctgata ctggaatgac ggatccactg
     3001 agatttggtg tgcgggatcg ctacgccaaa accaacgccg gtggtaacaa tctcttcccg
     3061 ctgccagaca tcttcttcca gctcaaacgg atgttcagtg cgcccgttaa cgccgaggtt
     3121 gccgcacagg aactggatcg cctgctcttt attgctaaaa tcctgatcaa caaagatatt
     3181 ctccagcgcc agcagtgggc gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc
     3241 aatttcctgc gcactgcggc attcacatgc ctgacgcgcc agttcccgac acgcctcgct
     3301 atccagttga cgaagctggc ttttcaccgc cggaatacgc gggctactca tactcagctc
     3361 atccaggccc agcccaagca gtagcggcag ataacggctt tcaccgccca gttcaccgca
     3421 aatgcctacc catttgcccc gctgatgcgc agtggtaact atttgctgca acatgcgcag
     3481 gaacgatggc gtaatcgggt tatatagcgg cgatacgcgc gggttattac gatcgaccgc
     3541 atacagatac tgggtcatat cgttggagcc gatactgaag aaatcgacct catcgcagaa
     3601 gtggtcgatg atgtagcaca ccgacggcac ttccaccatg atcccaagcg taatcgtctc
     3661 tgcatgacgc aggccatcgc gcttaagctc aacgatcgct ttttggatct cgcctttcac
     3721 ccataagatc tgatcgaggc tgttaaccat cgggatcatc aactgggcgt tgccgaaact
     3781 ggcggcgcgc aaaatggccc gcagttgagt gcggaacagg ccagcaaatt ccgggtaaat
     3841 acgtaccgcg cgatagccga ggaacgggtt ctcttcctgg ggaatattca gataaggaat
     3901 gcttttatcg ccgccgatgt ccatcgtgcg gaagataatc ggcttgtcgc ccgccgccag
     3961 tagcacctgc tggtaggctt caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat
     4021 atagagcatt tcggtacgga acagcccgac accttccgcg ccgttggcaa acgcgcctgg
     4081 cgcttccaga gcggtgccga tattcgccgc aatatcaata cgcttgttat cacgggaata
     4141 ggccagctgc gcggcagcct gcgcctgttg tttttggcgt ttatccgcca gcgtctgcgc
     4201 gacctgataa taaccgctca ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc
     4261 gtcaagcact gcaggttgcc cggcataacg ggcaatcgca tccagcggca agccactcag
     4321 taccgggatc gccgaggcac gggccagaat cagtgtatgc gaggtgcgcc cggttttctc
     4381 cagaatcatg cccgcaagat ttttcaaatc aaggctcaaa aactggcttg gggttaaatc
     4441 ttcagccacc agaatggtcg gtttttccag caccagattg ttgcgcggct tcagttccgg
     4501 ctaggtgata tgcagcaact gttcgctgat atcgcgaatg tcgctgaccc gctcgcgcag
     4561 ataatcgctg gcagaagcag atagtttggc gcaaacctgc tccatattgc tgatgatcgc
     4621 cgcccccagt ccctgatgct gttctgtcat caggcgacgg atattgcctg caaattcatc
     4681 atcctgaatc agcgacaaat gagcgctgag gatagttttg ctttcgccgt cacgctcacg
     4741 caattgctgg ttcagttgct cggcaagcgt tgccaggctg tgctccagcc gggtggaatc
     4801 ttgcgcactg gcggggattg cccgataact gtcgaggctg tcgctttgta acagggtcag
     4861 cgtacccacg ccgacgccgc ttgccagcac attgccgtac agtaaatccg ggttaaggcg
     4921 gcttaatgaa cgcggcagcg gatgcgccgt cagttctgcc tgcgtcggct gaacgctgtc
     4981 gctgtcgata aagcgcacca ggatgtactc ttccagcacg cgccgcgcct gctcttcatc
     5041 gctgccgcta atgttcaggc tgcaactgtc attaaataag gtgccagttc caatcaacgc
     5101 cagcgagctt ttagcatcgg cctttgcgtt ctggcgatgg ttgataaagg tgatttcgct
     5161 ttgccactgg ctgcactgtt ctttaagttc ccacgccgga cgagcatgta gaccgttagg
     5221 caaaggacag agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct
     5281 gaaatccgtc agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag
     5341 ttgctccatc tgcaaaatgt cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg
     5401 ggtggcaggc cccattacca ctgtgggtac gccagtatcg gttaagtgca ccgctccacc
     5461 gtccgtgccg ccgttactga acatatccgc ctgcaacgga acgccaattt ctgccgacac
     5521 ggtttcaatc cacgcggtaa gttttggcgg cgcaatcagc gatttatcgc ttaacaccag
     5581 catcggcccg ttaccaatct gacgatggtt agccgcgcca taatcaaagt ttttcgccca
     5641 gcaggcggta tcaagcacaa tggcgacgtc cggcgacacc gcgcgggtag cagtttgccc
     5701 gccgcgtaat cccacctctt cgctggaact tgccaccagc cacacttccg caggcagttc
     5761 ggcgtcgtgc agctcacgta gtaacgtcac cagcagatag caaccgaggc ggtcatcaaa
     5821 ggctttcccc atcacccgct ggtgagggag aacctgaaaa gctgtatcaa aggtgacgcg
     5881 atcgcctgga cgaattcccg cctgcatcac ttcgtcatac gagcgcgcac caatgtccac
     5941 gcgcatggcg ctgacgtcat tcccctgccg gtcgccgtca agcaggcctg gaattttgca
     6001 ctcttcacgg gtggtgatgc gcaccggctg cagctggcgg gcagccatgc gcacgttgcc
     6061 aaccggcagc acatcaattg ccccttcgcg ggagatgctg cgcaccataa atcccacttc
     6121 gtccatatgc gcacagatca tcaccttcgg acctgtcgat tcattgaggc ggatcagcac
     6181 cgatcccaga ccatcaaatc gtacttcttt ttgcaggcga tccgcttctt ccagtaggat
     6241 ctgccgcact tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg cttttaatag
     6301 cgataaatcc attatgcctc tcccgtgagc aacactgttt tcggcatggc gtagagcact
     6361 tctgcgcctt gcggggtaac cagcacaaca tcttcgatgc gcacgccccc ttgccctggc
     6421 aaataaatcc ccggctccac ggtcagtaac atgcctggct gtagcgtcgt ggtatcccgc
     6481 ggtgaaaagc gtggatcttc gtgtacttca ataccgatag catgaccggt gttgtgacca
     6541 aaatagtcgc caaatcctgc ctcggtaatc acccggcgcg cggcgtcgtc aacctgttag
     6601 cagcgtacgc cagggcgaat cgcggagatt gccgccagct gtgcttgcag gacaatctga
     6661 tagacgttaa acagcgggtg agattcggca attacccctt cgccattcac cagcaaggtg
     6721 cgcgtcatat cagagcagta cccctgatag agcgcaccaa aatcaagagt gacaaactcg
     6781 cctgctgcaa caatcttgtc gctggctttg ccgtgcggca gcgccccacg ccagccgctg
     6841 gcgacaatgg tgtcaaaaga ggctttttct gcgccctgct ggcgcataaa ccactccagt
     6901 tcagcggcta tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct
     6961 ccgcgatcgg caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt
     7021 tgccgcagca catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg
     7081 tgcgcggttt cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg
     7141 gcaatgattt gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg
     7201 tagccttgcg cacgggcttc cacctcaacg aaatagcgcg aatctaccag aatgtgcgca
     7261 ctttcacggc taataaccac atagcctgat ccggtggaga tccccagatg cggctgtttg
     7321 ttctgccgtg aggagagaag cactgcatcc agttgttgcg ccttaagcca gtcgcgcagc
     7381 gaagcgagta atgtcatctg gattttcctt cttacaggct atcgattaac aatttacctt
     7441 tacggaacat catcagacgc aggaacacca ccatcagcgc agttatgact gcccccagcg
     7501 cgatccccgc catatagacg cccaggttag tgaccagcgg ccatgcccag atagcggatt
     7561 ccgggaacca ttgcactgcg cccagccaga cagcggcggt tgagccgaca atcgcgccga
     7621 ccatatacga cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac
     7681 taatccccat aaaggcaagg aacatcgcgg tcttaccctg cggataaagt tgagcgttga
     7741 acaggcgttt gccggttaaa cgacggtcga taatggtcgc cagacccaga cctatcggcg
     7801 gaataacgat agcgatagaa cgtgcagtga ccggtaatac gtggtcagtg gtaaagctga
     7861 aggcaacaaa acctgcggct ttgttgatcg ggccaccaag gtcgattgcg gtcgccgcgg
     7921 cgatccccat tgcgtacatc agcgcgccct tctcacctgc ggcagtcagc acggtacgga
     7981 taccgccgtt gatccagccg ccaaaggggg taatgacgta gtacatcgcc agcatgacaa
     8041 aaatggcgga aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatccggta
     8101 actggatttt ctggttcatc cacttcacaa ggtaacccgc gacgatagaa ataatcagcg
     8161 caccaatgaa ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga
     8221 agttcagcag ttgcgtcggc tgggtagaca tcaagccgcc gataaaacca gccgggaacg
     8281 ccagtttgcc accgatagag ttcgccacaa aagcagcaaa catcggaatg gcaaaaccaa
     8341 acaacacgcc gccgaaggac tgcgacagcc aggcaaattt cagtaaggag aggtcgaaac
     8401 cggagaattt cccgctatta agagcatcca tgatgccgat ctcagcagga attttaagcc
     8461 agctataagc aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca
     8521 ccatgcgtga aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag
     8581 aggcctgcgg attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac
     8641 tgcgtttttt aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa
     8701 tcatctcttc gatttctttg atgatgcccg cagcgttttt aattgcgtcc tgcaaagtga
     8761 tttcataaac gtcgcgtgat tcgaaacgct cattatcttc cggggtaact gccacggagt
     8821 ggatgatgat ggttgcttcg gcgatatcct gcgccgtcag gcgattctgg ataccgtccg
     8881 cgccctgggt ttcaattttc acttcataac ctgcttctac cgccgcttct tccagcgcct
     8941 gagcggccat aaaggtgtga gccaggccca ttgggcaggc acataaggca attagtttct
     9001 tactcatcgt taattcctcg ttcagtgata gttgacgagt gaaattatga acgtgcaaaa
     9061 caaatcgccg ttaccagaca aataatgcat ttactggaga attaatcccg ctgtacggaa
     9121 gtgtgacgca ggtcggcctt gtgaggggga cacaaagaaa aattgaatta actgtatgat
     9181 ttaaaagatt atcgggagag ttacctcccg atataacaga aaggatttac agaatgtgcc
     9241 ctaaggtctg gcgtaaatgt gcaccggaac cgagaagacc cggattgtca tggacgatga
     9301 gatacaccgg aatatcatgg acatattctt taaagcgccc tttatcttca aatgcggcac
     9361 ggaaaccaga ggctttgaag aactcaagga agcgcggcac gataccgcca gcaatataaa
     9421 ccccaccaaa ggttcccagc gttaatgcca gattgccgcc aaaacggccc ataatgacgc
     9481 aaaacagcga caacgcacgg cggcaatcgg tgcagctgtc agccagcgcg cgttcggtaa
     9541 tatcttttgg cttgagattt tctggcaggc ggttgtcggc tttaacaatt gcgcgataca
     9601 aattcaccag cccaggacca gaaagcacgc gttccgccga aacatgacca atttccgcac
     9661 gcaatatttc gaggataatg gcctcttctt cactattcgg cgcaaaatca acgtgaccgc
     9721 cttcgcccgg caagcttacc caacgcttat cgacatggac cagatgcgca accccaagcc
     9781 ctgttccggc accgtaaacc gcaataggtt taccttcgac cggttctgcg ccaccaaact
     9841 gaatcagatg ctcttttttc agcatcggga tcgccatcga tacagcggta aaatcgttaa
     9901 taatttccag atggctaaaa ccgagattct ttttcatttc ggcaattgag aacgcccagg
     9961 tatggttggt cattgccacc cagtcaccgg taattgggca agcgatggca atacagccgt
    10021 ctttcacctc gaccttatgt tcttcaagat aaacgcgaat gaccgcttcg aggctggggt
    10081 aatcaagccc tgaataggtc ttagcctgcg agatttcacc actggcaata tcacacagag
    10141 caagacgtgc gttggtgccg cccacatcac cgactaatgc atactttgtc attcttcaac
    10201 tgctccgcta aagtcaaaat aattctttct cacactgtaa atacctgggg gcataacaac
    10261 aacgccggaa aggcaggctc cctgtaaata tcgatctggg tcacacattt actttatcgt
    10321 ttcagcacca attgcagcga tgcctttttg caagctgggc aaactaagta tctgaccccg
    10381 cataaggaat agaacatgct ccatccgcga gccagaacca tgttgttatt atcgctcccc
    10441 gccgtggcaa ttgggattgc gtccagtctt attctgattg tggtgatgaa aatcgcctcg
    10501 gtattacaga atttgctctg gcaacgactg ccgggaactc tggggatagc ccaggattca
    10561 cctatctgga tcatcggtgt attaacgcta acgggtattg cggtggggtt ggttatccgt
    10621 ttcagccagg gtcatgccgg accagacccc gcctgtgaac cgctgatcgg cgcaccagtt
    10681 ccgccctctg cgctaccagg acttatcgta gcattaattc tcggtcttgc tggcggcgtc
    10741 agcctggggc cggaacatcc gatcatgacc gtcaatatcg cccttgccgt cgccattggc
    10801 gctcgtctgt taccgcgcgt caaccgaatg gagtggacta ttttagcctc tgccggaacc
    10861 atcggcgcgc tgtttggtac acctgttgcg gcggcgttga tattttcgca aaccttaaat
    10921 ggcagtagtg aagttccgct atgggatcgt ctttttgcgc cgttaatggc ggcagcagct
    10981 ggtgcactta ctaccggatt atttttccat ccccattttt cactgcccat tgcgcattac
    11041 ggacagatgg aaatgaccga tattctcagc ggtgcaattg tcgcggcgat tgccatcgca
    11101 gcagggatgg tcgccgtatg gtgcttacca cggttgcacg cgatgatgaa tcaaatgaaa
    11161 aatccggtgc tcgtgctggg tattggcgga tttattctcg gaattctggg ggttattggt
    11221 ggaccagttt cgctgtttaa agggctggat gagatgcagc agatggtggc aaatcaggct
    11281 ttcagcacca gcgattactt tttgctggcg gtaattaaac ttgccgccct ggtcgttgct
    11341 gccgccagtg gctttcgcgg tgggcgaatc ttcccggcag tgtttgtcgg cgtggcatta
    11401 gggttgatgc tgcatgagca cgttcccgcc gttccagcgg caataaccgt ttcctgcgct
    11461 attctcggca tcgtgctggt ggtaacacgc gatggctggt taagtctttt tatggcggca
    11521 gtcgttgtac ccaataccac attgctaccg ctgctctgta tcgtcatgct tccggcatgg
    11581 ctgttattag caggtaagcc gatgatgatg gtcaatcgtc cgaagcaaca gccaccccac
    11641 gataatgttt agcaaaaaat gctctccttt atgatttaag agttatggcg atgatacggg
    11701 gctttacctc cccgtaatat tgcgttaaca ggccgctgac ggcttcgatc gtgaaggaga
    11761 ataacaatgt tcaggtcact gtttctggcg gccgccctga tggcatttac cccgcttgca
    11821 gcaaatgcag gtgaaatcac cctactgcca tcgatcaaat tacaaattgg cgatcgcgat
    11881 cattacggta attactggga cggtggtcac tggcgagatc gtgattactg gcatcgcaat
    11941 tatgaatggc gcaaaaaccg ctggtggcgt catgataatg gctatcatcg tggctgggat
    12001 aagcgtaaag catatgagcg tggctatcgt gaaggctggc gcgatcgtga cgatcatcgc
    12061 ggaaaaggcc gcggacatgg gcaccgccat taaacgcgtc ctccaatgga gcacaatgcc
    12121 tgatgcgacg ctagcgcgtc ttatcaggcc tacaaaccgg ctctttaacg cgtagggcgg
    12181 ataaggcgtt tacgccgcat ccgccaatag tgccagatgc gacgctcatt caactacaat
    12241 cccagcgccg tccccaccaa caaccagata ttcagcgcca cgaccagcac tacaatcacc
    12301 cagcctgtct gttttacgcg tttgctgttc accagatcgc ccatcaactt gctgtcactg
    12361 gtgaaaatca gcagtggaac cagcgccagg gcgataccaa aacttaacag cacctgactc
    12421 ataaccagaa tccgtgtcgg atctaatccc atcagaatga caataaatga cggcaacatg
    12481 gtgactgtac gacgcaccca cagcgggata tggaagcgaa tgaatccctg catcaccacc
    12541 tgccccgcca gtgtccccac caccgttgag gacagtccgg cagcaaccag acttaaccca
    12601 aagaccgttg ccgcagcatg gcttaacagc ggttgcagcg tcagataagc ctcatcaaga
    12661 tcggcaacac cagtatgacc agaaaagtgg aacgccgccg cagctgtagc catcatcgcc
    12721 agattgacaa aaccggcaat cgtcatggcg atagccacat cccatttggt ggcggaataa
    12781 cgttgttgac gcgaaccgcc atgtaaatgc tgagtgagcg aagagtgcaa ataaatcaca
    12841 tgcggcataa tcgtcgcccc taacacgcct gctgccagga agaccgcctc cgaagtaggt
    12901 aaactcggga tcaccattcc tttacccagc tgcgccaggt taggctggga gaaaatcaac
    12961 tcgacaatgt aagccgcggc aacaaacaac agtaacccgc caatcacttt ctccagtggt
    13021 ttttgcccgc gacgttgcag cattaaaatc aggaaagtcg cgatccccgt cagcaccgcg
    13081 ccctgcaaca acgagacacc aagaatgagt ttaaaaccga tcgccgcacc aataaattcc
    13141 gccaggtcgg ttgccatcgc aataatttct gcctgaaccc aatagaacca cactacggga
    13201 cgcggatagt gatcacgaat ctgctccgcc agattttttc cggtggcaat ccctagtttg
    13261 gcagagagga tctgaatcag catcgccatc aggttggccc aaacgacaac ccacagtagc
    13321 tgatagccga agctggcacc cgcctgaata ttggtcgcaa agttaccggg atcgatataa
    13381 ccaatcgccg caatgaacgc aggtcccatt aatgcgagcc tcatcttgcg cgccgcccgt
    13441 ccgctgctac tctcaacgcg atagttcgtc atcttgtgcc tctaaaacat agcctttgct
    13501 atgtttcatg ctatgataaa cgagaatgat tatcaaattc atttaaatgg attgtggtga
    13561 tttctctgat agaccaggat tatgactacg aaaagattga tgacttgaat gttatggtta
    13621 tgtttaatgt tagcacattt acataacttt cagcttccat acacaacata gcagaaatgt
    13681 atgacagatc actatttttg aagcctgtca caggacgtca ttatagtgtg tgtcagatct
    13741 cgttttcctt aaccatgtta catagaatgt gcacggaaat ttaacctgcc tcatatttgg
    13801 agcaaatatg gaccgcgtcc ttcattttgt actggcactt gccgttgttg cgattctcgc
    13861 actgctggta agcagcgacc gcaaaaaaat tcgtatccgt tatgttattc aactgcttgt
    13921 tatcgaagtg ttactggcgt ggttcttcct gaactccgac gttggtttag gcttcgtgaa
    13981 aggcttctcc gaaatgttcg aaaaactgct cagatttgcc aacgaaggga ctaacttcgt
    14041 ctttggtagc atgaatgatc aaggcctggc attcttcttc ctgaaagtgc tgtgcccaat
    14101 cgtctttatc tctgcactga tcggtattct ccagcacatt cgcgtgttgc cggtgattat
    14161 ccgcgcaatt ggtttcctgc tctccaaagt caacggtatg ggcaaactgg aatcctttaa
    14221 cgccgtcagc tccctgattc tgggtcagtc tgaaaacttt attgcctata aagatatcct
    14281 cggcaaaatc tcccgtaatc gtatgtacac catggctgcc acggcaatgt ccaccgtgtc
    14341 gatgtccatc gttggtgcat acatgaccat gctggaaccg aaatacgtcg ttgctgcgct
    14401 ggtactgaac atgttcagca cctttatcgt gctgtcgctg atcaatcctt accgtgttga
    14461 tgccagtgaa gaaaacatcc agatgtccaa cctgcacgaa ggtcagagct tcttcgaaat
    14521 gctgggtgaa tacattctgg caggtttcaa agttgccatt atcgttgccg cgatgctgat
    14581 cggctttatc gccctgatcg ccgcgctgaa cgcactgttt gccaccgtta ctggctggtt
    14641 tggctacagc atctccttcc agggcatcct gggctacatc ttctatccga ttgcatgggt
    14701 gatgggtgtt ccttccagtg aagcactgca agtgggcagt atcatggcga ccaaactggt
    14761 ttccaacgag ttcgttgcga tgatggatct gcagaaaatt gcttccacgc tctctccgcg
    14821 tgctgaaggc atcatctctg tgttcctggt ttccttcgct aacttctctt caatcgggat
    14881 tatcgcaggt gcagttaaag gcctgaatga agagcaaggt aacgtggttt ctcgcttcgg
    14941 tctgaaactg gtttacggct ctaccctggt gagtgtgctg tctgcgtcaa tcgcagcact
    15001 ggtgctgtaa aaccatacat aaaaaagccg gggatcattc cccggctttt ttacgcctgt
    15061 taatcagcta atggctgcgg gcgaccaatc aaataccctt gcagatattg caccccgagc
    15121 ttatgcaata gcgcctgctg ctgttgcgtc tcgacaaact ccgcgaccac actcaatgac
    15181 ttcgctttcg ccagatcggt aattgatctc acaatcatcg catccagcgt attcgtgaca
    15241 atatctttca caaagacgcc atcaattttg atgatatcag cctgcaaacg ctttaaccgt
    15301 tcgtagttgg catatccggt gccaaagtca tcaatcgcaa tccggaaacc aaacttatgc
    15361 agctgctcga tgttgtacat gctgctttct gcgttagaaa acgcctgctc ctcggtgatc
    15421 tcaagaatga ccgcctgcgg ggagatgtga taacgcttaa acagacgaat aatccgcccg
    15481 gcaatattct tttgcagcag cgtgagcggc attaaattga ctgaaaagcg cggtcctttt
    15541 ttgtcgcaag ggtgtgttgc cagccacttc aacaaggatt ccagcacttg caaatcaaaa
    15601 cgcgcgctaa ggttaaactg agcaataagg ggcagaaact tatccggggt cataatgccg
    15661 ccgtcatatt tcagtcgcgc gaggatctca tcataacctt caccctcttt gttgcgaatt
    15721 ggctgggcgt agagcagcaa atcaccctga tctaacgccg tgcgaatggt attcagcaat
    15781 agcacctgtt tagtggtctg cccggaaacc atctcctctc tgctatccag cgccagcaca
    15841 tgatgatgtg cgcaggattg ctccgccagc cagcttaact gccccaacaa gggttgcagg
    15901 gtttcctgat ttccatcaaa acgcccccag gcagcaccat agcccatatc cagcccggta
    15961 ttgttccagt gaatttgccg actattcagg atgttaacca tatgctggag tcgcccttcc
    16021 gtttccggcc cgctcagcac taacagcagt tcactacccg gcaattgata taacttttcg
    16081 ttttcctgca tcaacggcag cagcgtacgg taaattgatc ggatacagtg aacgcgcatc
    16141 attaagccgt aatgacgact cataaactca agattatcaa tgcgcaggca gcaaaactct
    16201 tgcccgcctc ttgctccggc gcttgttcca acgcacggaa gttgggcagg agcgttaacg
    16261 gatcggtcag cgcctgcaaa tgccagcggc gattaagcca ttcactgcga tgataaatgc
    16321 gcaccatata gagcaggcaa acgctaaagg aaatcagcac cgcaagaata aatgccagcg
    16381 aatattcggt ttccacccct tgcaaaaagt tctggttgta attcagaagg caaagcgttg
    16441 aaacagccct ggtgagattt aaaaacggat agcgaagctt accgaccccg agggtaaaga
    16501 tgatgaagaa aacgggtacc aggtaaccgg caataaagtc gttttcataa ggtgtgcaca
    16561 acagaagtag aagcacgctt aacgctgcca gccagcttaa ggtaaatgcg cgtttctctt
    16621 tgcccaacga cggcgcgata tccctgcgcc acaatatctg cgcaaagtgg ggacttacaa
    16681 tcatgcgagt gagatagtag aaaagcatgt tgtaaataag cacagcggtg aaaaggctta
    16741 gcaaatcaac gaccgtgaaa atggcatccg catcgccgaa aaaggtggat atctttagcg
    16801 ggaaatcaaa gaagctcccc acaagataca tgctgcattt gatgccgatc ggcgtcacca
    16861 aaccaagcca gaacagacgt tgccagacat atcggctggt caatccataa cgccaacgcg
    16921 tccccagctg ccagcgtaat attgcgcagg cacacaacac gacaaacgtc tggctaccga
    16981 gcatgaccgc agtctgtaaa aaagagagat gaaagttcca cagattggtg caaaacatcc
    17041 cgcataaaac gggaacgaca ccgcgccagc caaagataaa caagattgac agcattacgc
    17101 acagtggcat ccacgccaga aagatataac tggaatggat aatggcaagt ggcgaaataa
    17161 aacgggatag ctgaataagt accacggtga gcgtaaacgc tagtgtgaat atcttgatat
    17221 tttttatcag gttatgctcc acaaacatga gtacactact ctcactgatc ggggttttca
    17281 ggcacacaaa taataagttt attcgcatcg cgaagcaaac agggggccag cctgttgccg
    17341 atttttcatc caatgatgct ttcaccaggc ggcactaacc cggacgtaga ctgtattaca
    17401 aaagcggcaa aaagcagaga caaaaaaccc ccgctttgca gcgagggttg gaaatttggt
    17461 ggagctaagc gggatcgaac cgctgacctc ttgcatgcca tgcaagcgct ctcccagctg
    17521 agctatagcc ccacgatgcg tttacgtacc aaatttgctg ggtgcaaaat ttggtggagc
    17581 taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc agctgagcta
    17641 tagccccgtc acgtaaagct tgtcgagttg acgggcggca tcatatgaat tccgcccgaa
    17701 tgtgtcaacg gcaaattgca acgtgtagtt tcaatcgctg aaaaatcagg caaatgaaca
    17761 attttggaaa accgctcgca ttcagtagtt attcatgtca cggtttcctg taaagtggtg
    17821 ttataaaatg aactactaat agacccacat acattcaggg aattgttatg ttcaaggaga
    17881 ggatgacacc agatgaactt gccagactga ccggctatag ccgccagacc attaataaat
    17941 gggtacgcaa ggagggctgg acaacgtcac caaaacctgg cgtccagggt ggcaaagccc
    18001 gactggttca cgtcaatgaa caggttcgtg aatatattcg caatgctgaa cgtccagaag
    18061 gtcagggaga agcgcctgcc ctttctggtg atgcgccgct tgaagtattg ctggtgacac
    18121 tggcgaaaga gatgacgcca gttgaacaaa aacagtttac atccttgctt ctgcgggaag
    18181 ggattatcgg attgttacaa cgcttaggga ttcgcgatag caaataatat gaaaagatta
    18241 cgcaataaaa tgaccaccga agaactggct gaatgcctcg gtgtggctaa acaaactgtt
    18301 aaccgttgga tcagagaaaa aggctggaaa acggaaaaat ttcccggcgt gaaaggcggt
    18361 cgtgccagac ttattctggt cgatacgcaa gtttgcgagt ttattcagaa cacgccagcc
    18421 ttccataaca caccaatgtt gatggaagct gaagagagaa ttgctgaata cgctcctgga
    18481 gcacgcgccc cggcgtatcg acaaatcatc aacgcgatag ataatatgac agacattgag
    18541 cagggaaaag tcgcgcaatt tttgtcacgt gaaggaattc gcaatttcct cgcccgtctc
    18601 gatattgacg aatcagcata aataaaaaac ggcaggatat tatctcctgc cgtttatctt
    18661 tttacacgct aattactgct gattttcgcg ttcagcaata aaatccagcg ctttgttgat
    18721 acgctcgata ctgcgggtct taccaattgc gtgaacggta acatccagtg ctggagactg
    18781 ccccgccccg gttacggcga cacgcagcgg cataccaact ttacccatac ccacttccag
    18841 ctcatccgcc gtcgcctgaa tagcgtgatg aacgttttca gcggtccagt cagtaatcgc
    18901 ggccagtttg tcacgaacca cttccagcgg ctgacgcgct accggacgca gatgtttttt
    18961 cgccgcatcg gcatcgaact cagcaaaatc ttcgtagaaa taacggcagc tctgtgccat
    19021 ctctttcagc gtcttgcagc gttcgcccag cagtttcacc agatcagcca gctgcgggcc
    19081 gttacgggta tcgatatttt cctgctcaat gtgccactgt aagtgagtag caacatactc
    19141 cggcggcagc gcgttaatgt agtgatggtt cagccacagc agcttgtcgg tgttgaacgc
    19201 actggcagat ttgctgacgg cattcaaagt gaagtatttg atcatctctt cacgagtgaa
    19261 gatttcctga tcgccgtggg accagcccag acgcaccaga tagttcagca gtgcttctgg
    19321 caaataaccg tcatcacgat actgcattac gctgactgcc ccgtgacgtt tggacagttt
    19381 tttaccgtca tcgccattga tcatagaaac gtgcgcgtaa accggcaccg gcgctttcag
    19441 ggccttaaga atgttgatct ggcgcggcgt gttgttgata tggtcttcgc cacggataac
    19501 gtgggtgatt tccatatccc agtcatcgac aaccacacag aagttatagg ttggggaacc
    19561 atcggtacgg cggataataa gatcgtccag ttcctggttg ctgaactcga tcggaccacg
    19621 gatctgatcg tcaaaaacaa cagaaccttc ctgcgggtta gcaaaacgta caacacacgg
    19681 ttcatcatca gcatgatgct catggctgtg gcggcagcga ccgtcataac gcggcttctc
    19741 acctttcgcc atttgctctt cgcgcagcgc ttccaggcgc tctttagagc aatagcattt
    19801 ataagcagtg ccctcttcca gcatctgatc gatcaccgcg ttgtagcgat caaaacgttt
    19861 ggtctggtag tacggacctt catcccactc caggctcagc cagttcatgc catccataat
    19921 ggcttcgata gcttccggcg tggaacgctc aagatcggtg tcttcaatac gcagcacgaa
    19981 ctcaccgccg tggttacgtg caaaaagcca ggagtaaaga gcagtacgcg cgccgccaac
    20041 gtgcagatag cctgttgggc ttggcgcgaa gcgagttttg attttcatga aatggcctta
    20101 cgtttagaaa gatgccgaca accggcaaat cctggaaaaa ttgagtgggc gatattctat
    20161 cactcccgcc tgattcctca atgtagttcg ggtttctacc ttacgcatta ttgttttcgt
    20221 tgacaaattg cgcaacacgg tctgttttgc gatcatcttg cttaaattta cgacgaacga
    20281 acaatttctt tagaaaatgc gttgactcat tttgaactct ccctataatg cggctccaca
    20341 cagcgggggt gattagctca gctgggagag cacctccctt acaaggaggg ggtcggcggt
    20401 tcgatcccgt catcacccac caacatactt tatgtagtct ccgccgtgta gcaagaaatt
    20461 gagaagtggg tgattagctc agctgggaga gcacctccct tacaaggagg gggtcggcgg
    20521 ttcgatcccg tcatcaccca ccaatttctc gccagctaaa tttcttgtaa aaatgtgaag
    20581 taccgaagtg ggtgattagc tcagctggga gagcacctcc cttacaagga gggggtcggc
    20641 ggttcgatcc cgtcatcacc caccacttcg ggtcgttagc tcagttggta gagcagttga
    20701 cttttaatca attggtcgca ggttcgaatc ctgcacgacc caccaatgta aaaaagcgcc
    20761 ctaaaggcgc ttttttgcta tctgcgatac tcaaagattc gaacctgcag caggtttgag
    20821 ttgagcgcag cgaaacaacg gagccgctcg cggcgacggc ccgaagggcg agcgaagcga
    20881 gtcatcctgc acgacccacc aatgtaaaaa agcgccctaa aggcgctttt ttgctatctg
    20941 cgatttgtaa gattgatttt tcttctcaag ttccttgttc ttctgccgat attacctcgt
    21001 acagtgacga ggactgaaca atggccacta tctcttcaac ttcaattcct tccatacaaa
    21061 cacagagtag taacagggca tcacaaggta gcgatgttgc ttcaaaaatc gcgcggataa
    21121 gtcagcaaat catcaaactc actcaacaaa ttaaagagat tgtagacact agcggcagtg
    21181 ctgaagacaa acaaaaacag gccgaattga ttcaacagca aatcacccta ctggaaacac
    21241 aactggcgca gttgcagaga cagcaggcag aaaaggcgca ggagaaagag cagcgtttgt
    21301 ctctgaatgt atcccttcta aatcctgttg aaaatacgac tcatatcgat atctatatct
    21361 gatacaacgg agccccttcc cgaccagcca ttagcgcatt gccctggtct cgtaccacgt
    21421 cccataacgc ttcagcggcg gtggaaagtg aacggttctt cctgcgcacc agcatcaact
    21481 ggcgttcaac aaccggcgta atgcgcttta ccaccagtgg gctaccttca ggcaatggta
    21541 gcgccagtgc gggaagaata ctgatgccga tgcctgcggc taccatcggg aacagcgtcg
    21601 ccggatgtcc aatctcctgc acaatattcg cctgaatacc attgcgcgcc agcgctgcgt
    21661 caatcagcgg tcggctgcct gacgcgtaat cctgcaacac caatttcgct ccctgtaatg
    21721 cctgccaggg tacataatct tccacggcca aagcgctatc gcgatggcaa agcagaaaga
    21781 aaggctcgga aagaatcgct tcacattgca gatcgcccac tgggcctgga tcgatgacga
    21841 tgcaaaaatc gacatctccc tggcgaatac tttccatcac ccactgctgc ggacgatcgt
    21901 gcaggacaaa ctgaatatct ggatagcggc gatggctttc agcaatgcat tgcggaataa
    21961 gatgcgcgga aatggtctgg ctggcagcga cccgaacttt tccgctcagt tgttgcccca
    22021 tacgcccggt atcgcgcaac gtgctgttaa gttcatccag cagtcgctca agacgcaaag
    22081 ccaactgctg ccctgcatct gtaagcacca cttcacgcgt ggttctgtcc agcaggcgaa
    22141 caccagtatg attttccagc tccttcacac tgtgacttac tgccgactgg ctcaagccga
    22201 tacgctctcc tgcacgacta aaacttttct cctgcgctac tgtgacgaaa acctttaatt
    22261 gttttaaaga ataattcatc tgttttcttc atgaatggat gcaataaatc aattttattt
    22321 ctcaaactgg aagaagcaca atagaaccat cgatcatctg gagtctttat gaaacttttt
    22381 cgtatcctcg atcctttcac cttaaccctg atcacggtgg tgttgctggc ctctttcttt
    22441 ccggccagag gcgatttcgt ccccttcttt gaaaatctga ccaccgcagc tattgccctg
    22501 ctgttcttta tgcacggcgc gaagttgtcg cgtgaggcga ttattgctgg cggtggtcac
    22561 tggcgactgc atttgtgggt aatgtgcagc accttcgtgc tgtttccgat tctgggtgta
    22621 ctgtttgcct ggtggaaacc ggtaaatgtc gacccgatgc tctactccgg ttttctctac
    22681 ttgtgcattc tcccggctac cgtgcagtct gcaatcgcct tcacgtcaat ggcgggcggt
    22741 aacgtcgcgg cggcggtttg ttctgcgtca gcatccagcc tgctggggat tttcctttca
    22801 ccattgctag ttggtctggt gatgaatgtt cacggtgcag ggggcagcct tgagcaggtc
    22861 ggtaaaatta tgctgcaact gctgctgccg tttgtgttgg ggcatctttc ccggccgtgg
    22921 attggtgact gggtgtcgcg caataaaaaa tggattgcga aaactgacca gacgtccatt
    22981 ctgttggtgg tttatacagc gttcagcgaa gccgtcgtta atggtatctg gcataaagtt
    23041 ggctggggat cattgctgtt tatcgtggtg gtcagctgcg ttcttctggc tatcgtgatt
    23101 gtagttaacg tctttatggc acgccgactg ggcttcaata aggcagatga aattactatc
    23161 gtcttttgtg gttcgaaaaa gagtctggca aatggcatcc cgatggcaaa cattctgttc
    23221 cccacatcgg tgatcggtat gatggtgctg cccctgatga ttttccatca gatccaattg
    23281 atggtctgtg cggtgctggc gcgtcgatac aaacgccaga ccgaacagtt acaggcgcag
    23341 caggaaagca gcgccgataa agcttaaagc ggacgcttca ggggctggac cagttgcgtc
    23401 agcccctcgg ttttaatcag cagcgtgatt tgcattagct caccgagctt ccctgccggg
    23461 aactcatctt tgcgggcaaa ccacagcaga tactcctccg gcaggtcgat taagcgacgc
    23521 cctttgtatt tgccaaacgg cattatcgta ttggctattt caatcagctg ctctttttcc
    23581 atctcagcta cccagcaaac gcagcatttc cgcttcgtcg atgacttcaa tgcccagttc
    23641 ctgcgccttc gccagtttag atcctgcagc ttcacccgct atcaccagat cggttttctt
    23701 cgacacgctg cccgcgactt tcgcgcccag ttcgaccagt cgagctttag cgtcatcacg
    23761 cgacatctgg cttaagctgc ccgtaagcac cacggtttta ccagcaaacg ggctgtcaat
    23821 ctcttccgcg ttgataacga tcggcgcagg ccagtgaaca ccttccgcca acagctcgct
    23881 gatgacattg cggttgcttt cttcggcaaa gaagttgtga acgtgggatg caacgacaat
    23941 gccaacatca ggcacctttt gcagctcttc aatcgaagcg gcttccagca cttccagcgt
    24001 gccgaaatat gccgccagac ctgctgcggt ggcctcgccg acttcacgga tgccaagtgc
    24061 atagaggaag cgagcaaagg tggtttcttt cgctttttcc agcgcgttaa ccacgttttg
    24121 tgccgatttt ggccccatac gctccagtcc ggtcagtttg cctgcggtga gtttgaacag
    24181 atctgccgga gtgtggacat attctttttc aaccagctga tcgatgattt tgtcgcccat
    24241 tccgtcaaca tccatcgcac ggcgggaaac aaagtgtttc agcgactctt tacgctgcgc
    24301 accgcaaatc aggccaccgg tacagcgggc aaccgcttca ccttccacac gctcaacgtc
    24361 agaaccacat accggacaat gcgtcgggaa tacaacctca cgggtatctt ccgggcgttc
    24421 agaaagcacg acgttaacca cctgcgggat cacgtcgcca gcgcggcgaa tcaccacttt
    24481 atcgccaatg cgtaaaccaa gacgttcgat ttcatccgca ttgtgtaagg ttgcgttact
    24541 caccagcacg ccagcaacat ggacaggttc cagacgcgca acaggcgtaa tcgcgccagt
    24601 acgcccaacc tgaaactcga cgtcacgcac aaaggtcatc tgctcctgcg ccgggaattt
    24661 aaacgctacc gcccagcgcg gggcacgcgc gacaaagcca agctgctcct gctgttccag
    24721 tgagttgacc ttaatcacca cgccgtcgat atcaaagccc agcgtcgggc ggtcttcttc
    24781 cactttgtga tagaacgcca gcacttcttc cgccgattca caaagcgtta cccgatcgct
    24841 gaccggcaac ccccactttt taaattgcag taaacggcca agatgagtat ccggcagctc
    24901 gccaccttcc agaacaccaa cgccatagca gaaaaaagtg agcggtcgct tcgctgtaat
    24961 acgcggatca agctgacgca gtgaaccagc tgccgcatta cgtgggttag caaaaacttt
    25021 cccgcccgtg cgtcgcgcat cttcgttaat cttttcgaac cccgcctgcg gcaggaacac
    25081 ttcaccacgc acttccagac gcgccgggat attctctccg tgcagcttca gcggaatggc
    25141 gcgaatagta cgcacattag acgtgatatc ttccccggtg gtgccatcgc cacgggtcgc
    25201 ggcactgact aaaacgccat tttcatacag aatactgacg gcaaggccat ccagcttcag
    25261 ctcacagcac caggtgactt tctcgttgtt tttcagacgg tcctgcacac gtttgttgaa
    25321 agcaagaaag ctttcttcat caaaaacgtt atccagtgac agcattggta cttcatggcg
    25381 tatctggctg aaagccgcca gcggcgcagc acctacacgt tgggtaggcg aatcaggcgt
    25441 aatcagttct ggatgtttgg tttccagctc gcgcagttcg cgcatcagcc tgtcgtattc
    25501 agcgtcggga atttccggcg catccatcac atgataaaga tattcatgat ggcgaagcgt
    25561 cgttcgcagt tctgtcagtt gttgttcgat tgattccata tcgcaccatc aatgctaaaa
    25621 acccccgaca agcgggggtt cgaagaggag ttaatttgcc ttaagtgtat caggcgttgg
    25681 cgtctttgac ttcgcggatg atgtcctggt actcgcgcaa tttctgcgga gtcatcatac
    25741 ggcgctgatc gtcaagcacg acaccgccca cttcatcggc aatatgctgc gcagattgca
    25801 gcatcagctt gaagttctgc agctcgtcac cgtaagacgg tacctgcata aagatagtga
    25861 cacccggagt agtgaaatcc ttcatttcag gatcaaaggt tcccggtttc accatattcg
    25921 ccaggctgaa taacgccggg ccgctgccat ccgggctaag atgacgatgg taaatattca
    25981 tatcgccaaa aatgaagccc gcttgttgaa tgctgttaag aagcagttca ccgtttagct
    26041 cgctaccgtg atgcgccgcg acgttcatga taatcaccgc ttctttgcgc ttcggtttat
    26101 ccataactgg agccggttcc gctacaggct caggctgtgg tgccgctacg ggttctgcag
    26161 gctggaaagc ctgttgtgcc ggttgcggtg ctgaatgcac aggctgcggc gctggcgcga
    26221 cctgtggcga aactggctgc tgcaacggct gttcaggctg cggctgatag gcaggctgct
    26281 gcatcggctg cgctggacgc ggagcatgtt gcggcggtac ctgcgcttca ggcggctgct
    26341 ggatcggttg acgcggctgc gcagacgcat aaggcggttg gtactggtgt tgcggcgacg
    26401 gacgagcagc ctcatgctcc tgagcgttag ccggggcatg attcacgcgg tgaacacgaa
    26461 cctcaccaac gccctcatca tcttcgacat cctcgtcata agaatcgtcg tcacgttttg
    26521 acttcattcg ttttaatggc cgatcgcgga acatagaaga tcgttcttta cggctggtcc
    26581 agaaaccatg taccagtaaa gcgattatgg cgatcgcgcc aacaatgatt aatatcagac
    26641 gcaaatcctg catcattata ttctctgttg ttctaacacc ttgccaccac ggcaaacatt
    26701 tactcactaa gagtatttgc cgattacctc aagtgcaagt gcactattaa ctttcacagc
    26761 acaaagatag atgaaatcgt gctttttgct gttttttcga acatatccta actgtccatt
    26821 gcgcaattac ccggtaaaat acgcagaatt ttcctgggat tggtcaaaag gagctcatcc
    26881 tgattatggt ttcatcattc acatctgccc cacgcagcgg tttttactat tttgcgcaag
    26941 gctggaagct cgtctcgcaa cctgggattc ggcgtttcgt tattttaccg ctgctggtca
    27001 atattttgtt gatggggggc gcattctggt ggctctttac acagctcgat gtctggatcc
    27061 cgactctcat gagttacgtt ccggactggc tacaatggct gagttatctg ttgtggcctc
    27121 tggctgtcat ctctgtgctg ttagtgtttg gctatttctt ctccacgatt gctaactgga
    27181 ttgccgctcc gtttaacggt ttattggctg aacaactgga agcacgattg actggcgcta
    27241 caccgccaga taccgggatt ttcggtatca tgaaagatgt gccgcgaatc atgaaacgcg
    27301 aatggcaaaa atttgcctgg tatctgccgc gcgcaattgt attactaatt ctttacttca
    27361 tccctggtat tgggcaaacc gtcgcgccgg tactgtggtt cctgtttagc gcctggatgt
    27421 tagccatcca gtattgcgat taccccttcg ataaccacaa agtgccgttt aaagagatgc
    27481 gcaccgccct gcgcacacgc aaaatcacca atatgcagtt tggtgcttta accagcctgt
    27541 ttacgatgat cccgctgctt aatctgttca tcatgcccgt tgccgtttgt ggcgcgacgg
    27601 cgatgtgggt cgattgctat cgcgataaac acgcgatgtg gcggtaacaa tctaccggtt
    27661 attttgtaaa ccgtttgtgt gaaacagggg tggcttatgc cgccccttat tccatcttgc
    27721 atgtcattat ttcccttctg tatatagata tgctaaatcc ttacttccgc atattctctg
    27781 agcgggtatg ctacctgttg tatcccaatt tcatacagtt aaggacaggc catgagtaag
    27841 atttttgaag ataactcgct gactatcggt cacacgccgc tggttcgcct gaatcgcatc
    27901 ggtaacggac gcattctggc gaaggtggaa tctcgtaacc ccagcttcag cgttaagtgc
    27961 cgtatcggtg ccaacatgat ttgggatgcc gaaaagcgcg gcgtgctgaa accaggcgtt
    28021 gaactggttg aaccgaccag cggtaatacc gggattgcac tggcctatgt agctgccgct
    28081 cgcggttaca aactcaccct gaccatgcca gaaaccatga gtattgaacg ccgcaagctg
    28141 ctgaaagcgt taggtgcaaa cctggtgctg acggaaggtg ctaaaggcat gaaaggcgca
    28201 atccaaaaag cagaagaaat tgtcgccagc aatccagaga aatacctgct gctgcaacaa
    28261 ttcagcaatc cggcaaaccc tgaaattcac gaaaaaacca ccggtccgga gatctgggaa
    28321 gataccgacg gtcaggttga tgtatttatt gctggcgttg ggactggcgg tacgctgact
    28381 ggcgtcagcc gctacattaa aggcaccaaa ggcaagaccg atcttatctc tgtcgccgtt
    28441 gagccaaccg attctccagt tatcgcccag gcgctggcag gtgaagagat taaacctggc
    28501 ccgcataaaa ttcagggtat tggcgctggt tttatcccgg ctaacctcga tctcaagctg
    28561 gtcgataaag tcattggcat caccaatgaa gaagccattt ctaccgcccg tcgtctaatg
    28621 gaagaagaag gtattcttgc aggtatctct tctggagcag ctgttgccgc ggcgttgaaa
    28681 ctacaagaag atgaaagctt taccaataag aatattgtgg ttattctacc atcatcgggt
    28741 gagcgttatt taagcaccgc attgtttgcc gatctcttca ctgagaaaga attgcaacag
    28801 taatgccagc ttgttaaaaa tgcgtaaaaa agcacctttt taggtgcttt tttgtggcct
    28861 gcttcaaact ttcgcccctc ctggcattga ttcagcctgt cggaactggt atttaaccag
    28921 actaattatt ttgatgcgcg aaattaatcg ttacaggaaa agccaaagct gaatcgattt
    28981 tatgatttgg ttcaattctt cctttaacgg cataatgttt aatgacgtac gaaacgtcag
    29041 cggtcaacac ccgccagcaa tggactgtat tgcgctcttc gtgcgtcgcg tctgttaaaa
    29101 actggcgcta acaatacagg ctaaagtcga accgccaggc tagactttag ttccacaaca
    29161 ctaaacctat aagttgggga aatacaatgt tccagcaaga agttaccatt accgctccga
    29221 acggtctgca cacccgccct gctgcccagt ttgtaaaaga agctaagggc ttcacttctg
    29281 aaattactgt gacttccaac ggcaaaagcg ccagcgcgaa aagcctgttt aaactgcaga
    29341 ctctgggcct gactcaaggt accgttgtga ctatctccgc agaaggcgaa gacgagcaga
    29401 aagcggttga acatctggtt aaactgatgg cggaactcga gtaatttccc gggttctttt
    29461 aaaaatcagt cacaagtaag gtagggttat gatttcaggc attttagcat ccccgggtat
    29521 cgctttcggt aaagctctgc ttctgaaaga agacgaaatt gtcattgacc ggaaaaaaat
    29581 ttctgccgac caggttgatc aggaagttga acgttttctg agcggtcgtg ccaaggcatc
    29641 agcccagctg gaaacgatca aaacgaaagc tggtgaaacg ttcggtgaag aaaaagaagc
    29701 catctttgaa gggcatatta tgctgctcga agatgaggag ctggagcagg aaatcatagc
    29761 cctgattaaa gataaacaca tgacagctga cgcagctgct catgaagtta tcgaaggtca
    29821 ggcttctgcc ctggaagagc tggatgatga atacctgaaa gaacgtgcgg ctgacgtacg
    29881 tgatatcggt aagcgcctgc tgcgcaacat cctgggcctg aagattatcg acctgagcgc
    29941 cattcaggat gaagtcattc tggttgccgc tgacctgacg ccgtccgaaa ccgcacagct
    30001 gaacctgaag aaggtgctgg gtttcatcac cgacgcgggt ggccgtactt cccacacctc
    30061 tatcatggcg cgttctctgg aactgcctgc tatcgtgggt accggtagcg tcacctctca
    30121 ggtgaaaaat gacgactatc tgattctgga tgccgtaaat aatcaggttt acgtcaatcc
    30181 aaccaacgaa gttattgata aaatgcgcgc tgttcaggag caagtggctt ctgaaaaagc
    30241 agagcttgct aaactgaaag atctgccagc tattacgctg gacggtcacc aggtagaagt
    30301 atgcgctaac attggtacgg ttcgtgacgt tgaaggtgca gagcgtaacg gcgctgaagg
    30361 cgttggtctg tatcgtactg agttcctgtt catggaccgc gacgcgctgc ccactgaaga
    30421 agaacagttt gctgcttaca aagcagtggc tgaagcgtgt ggctcgcagg cggttatcgt
    30481 tcgtaccatg gacatcggcg gcgacaaaga gctgccatac atgaacttcc cgaaagaaga
    30541 gaacccgttc ctcggctggc gcgctatccg tatcgcgatg gatcgtaaag agatcctgcg
    30601 cgatcagctc cgcgctatcc tgcgtgcctc ggctttcggt aaattgcgca ttatgttccc
    30661 gatgatcatc tctgttgaag aagtgcgtgc actgcgcaaa gagatcgaaa tctacaaaca
    30721 ggaactgcgc gacgaaggta aagcgtttga cgagtcaatt gaaatcggcg taatggtgga
    30781 aacaccggct gccgcaacaa ttgcacgtca tttagccaaa gaagttgatt tctttagtat
    30841 cggcaccaat gatttaacgc agtacactct ggcagttgac cgtggtaatg atatgatttc
    30901 acacctttac cagccaatgt caccgtccgt gctgaacttg atcaagcaag ttattgatgc
    30961 ttctcatgct gaaggcaaat ggactggcat gtgtggtgag cttgctggcg atgaacgtgc
    31021 tacacttctg ttgctgggga tgggtctgga cgaattctct atgagcgcca tttctatccc
    31081 gcgcattaag aagattatcc gtaacacgaa cttcgaagat gcgaaggtgt tagcagagca
    31141 ggctcttgct caaccgacaa cggacgagtt aatgacgctg gttaacaagt tcattgaaga
    31201 aaaaacaatc tgctaatcca cgagatgcgg cccaatttac tgcttaggag aagatcatgg
    31261 gtttgttcga taaactgaaa tctctggttt ccgacgacaa gaaggatacc ggaactattg
    31321 agatcattgc tccgctctct ggcgagatcg tcaatatcga agacgtgccg gatgtcgttt
    31381 ttgcggaaaa aatcgttggt gatggtattg ctatcaaacc aacgggtaac aaaatggtcg
    31441 cgcctgtaga cggtaccatt ggtaaaatct ttgaaaccaa tcacgcgttc tctatcgaat
    31501 ctgatagcgg cgttgaactg ttcgtccact tcggtatcga caccgttgaa ctgaaaggcg
    31561 aaggcttcaa gcgtattgct gaagaaggtc agcgcgtgaa agttggcgat actgtcattg
    31621 aatttgatct gccgctgctg gaagagaaag ccaagtctac cctgactccg gttgttatct
    31681 ccaacatgga cgaaatcaaa gaactgatca aactgtccgg tagcgtaacc gtgggtgaaa
    31741 ccccggttat ccgcatcaag aagtaattct tgccgcagtg aaaaatggcg ccca
//
LOCUS       DAEEKQ010000029        30157 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.029, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000029 DAEEKQ010000000
VERSION     DAEEKQ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.029"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..234
                     /gene="tuf"
                     /locus_tag="KLK57_001353"
     CDS             <1..234
                     /gene="tuf"
                     /locus_tag="KLK57_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE0891763.1"
                     /translation="GRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMVMPGDNIKMVV
                     TLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            464..847
                     /gene="secE"
                     /locus_tag="KLK57_001354"
     CDS             464..847
                     /gene="secE"
                     /locus_tag="KLK57_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HBE0891764.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            849..1394
                     /gene="nusG"
                     /locus_tag="KLK57_001355"
     CDS             849..1394
                     /gene="nusG"
                     /locus_tag="KLK57_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HBE0891765.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1553..1981
                     /gene="rplK"
                     /locus_tag="KLK57_001356"
     CDS             1553..1981
                     /gene="rplK"
                     /locus_tag="KLK57_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HBE0891766.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1985..2689
                     /gene="rplA"
                     /locus_tag="KLK57_001357"
     CDS             1985..2689
                     /gene="rplA"
                     /locus_tag="KLK57_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HBE0891767.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3102..3599
                     /gene="rplJ"
                     /locus_tag="KLK57_001358"
     CDS             3102..3599
                     /gene="rplJ"
                     /locus_tag="KLK57_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HBE0891768.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3666..4031
                     /gene="rplL"
                     /locus_tag="KLK57_001359"
     CDS             3666..4031
                     /gene="rplL"
                     /locus_tag="KLK57_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HBE0891769.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKSLEEAGAEVEVK"
     gene            4351..8379
                     /gene="rpoB"
                     /locus_tag="KLK57_001360"
     CDS             4351..8379
                     /gene="rpoB"
                     /locus_tag="KLK57_001360"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HBE0891770.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8456..12679
                     /gene="rpoC"
                     /locus_tag="KLK57_001361"
     CDS             8456..12679
                     /gene="rpoC"
                     /locus_tag="KLK57_001361"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HBE0891771.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12921..14054)
                     /gene="thiH"
                     /locus_tag="KLK57_001362"
     CDS             complement(12921..14054)
                     /gene="thiH"
                     /locus_tag="KLK57_001362"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HBE0891772.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEPLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(14051..14821)
                     /gene="thiG"
                     /locus_tag="KLK57_001363"
     CDS             complement(14051..14821)
                     /gene="thiG"
                     /locus_tag="KLK57_001363"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HBE0891773.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(14823..15023)
                     /gene="thiS"
                     /locus_tag="KLK57_001364"
     CDS             complement(14823..15023)
                     /gene="thiS"
                     /locus_tag="KLK57_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HBE0891774.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15007..15762)
                     /gene="thiF"
                     /locus_tag="KLK57_001365"
     CDS             complement(15007..15762)
                     /gene="thiF"
                     /locus_tag="KLK57_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HBE0891775.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSHQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15755..16390)
                     /gene="thiE"
                     /locus_tag="KLK57_001366"
     CDS             complement(15755..16390)
                     /gene="thiE"
                     /locus_tag="KLK57_001366"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HBE0891776.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     NEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16390..18285)
                     /gene="thiC"
                     /locus_tag="KLK57_001367"
     CDS             complement(16390..18285)
                     /gene="thiC"
                     /locus_tag="KLK57_001367"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HBE0891777.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAVHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(18366..18466)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18519..18995)
                     /gene="rsd"
                     /locus_tag="KLK57_001368"
     CDS             complement(18519..18995)
                     /gene="rsd"
                     /locus_tag="KLK57_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HBE0891778.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            19090..19863
                     /gene="nudC"
                     /locus_tag="KLK57_001369"
     CDS             19090..19863
                     /gene="nudC"
                     /locus_tag="KLK57_001369"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HBE0891779.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19903..20967
                     /gene="hemE"
                     /locus_tag="KLK57_001370"
     CDS             19903..20967
                     /gene="hemE"
                     /locus_tag="KLK57_001370"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HBE0891780.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20977..21648
                     /gene="nfi"
                     /locus_tag="KLK57_001371"
     CDS             20977..21648
                     /gene="nfi"
                     /locus_tag="KLK57_001371"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HBE0891781.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGVDVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21691..22281
                     /locus_tag="KLK57_001372"
     CDS             21691..22281
                     /locus_tag="KLK57_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HBE0891782.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            22468..22740
                     /gene="hupA"
                     /locus_tag="KLK57_001373"
     CDS             22468..22740
                     /gene="hupA"
                     /locus_tag="KLK57_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HBE0891783.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22753..23448
                     /locus_tag="KLK57_001374"
     CDS             22753..23448
                     /locus_tag="KLK57_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HBE0891784.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALLLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23450..23875)
                     /gene="zraP"
                     /locus_tag="KLK57_001375"
     CDS             complement(23450..23875)
                     /gene="zraP"
                     /locus_tag="KLK57_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HBE0891785.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24113..25489
                     /gene="zraS"
                     /locus_tag="KLK57_001376"
     CDS             24113..25489
                     /gene="zraS"
                     /locus_tag="KLK57_001376"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HBE0891786.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLDAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGATFTLWLPVT
                     ITRKGPQG"
     gene            25486..26811
                     /gene="zraR"
                     /locus_tag="KLK57_001377"
     CDS             25486..26811
                     /gene="zraR"
                     /locus_tag="KLK57_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HBE0891787.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVSASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADRRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAVHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIAGTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26808..28097)
                     /gene="purD"
                     /locus_tag="KLK57_001378"
     CDS             complement(26808..28097)
                     /gene="purD"
                     /locus_tag="KLK57_001378"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HBE0891788.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGMKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVAGGKVFHAGTKLADNEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28109..29698)
                     /gene="purH"
                     /locus_tag="KLK57_001379"
     CDS             complement(28109..29698)
                     /gene="purH"
                     /locus_tag="KLK57_001379"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HBE0891789.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITATKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7058 a         7863 c         8309 g         6927 t
ORIGIN      
        1 ggtcgtcata ctccgttctt caaaggctac cgtccgcagt tctacttccg tactactgac
       61 gtgactggta ccatcgaact gccggaaggc gtagagatgg taatgccggg cgacaacatc
      121 aaaatggttg ttaccctgat ccacccgatc gcgatggacg acggtctgcg tttcgcaatc
      181 cgtgaaggcg gccgtaccgt tggcgcgggc gttgtagcaa aagttctgag ctaattgccg
      241 ataacatttg acgcaatgcg cactaaaagg gcatcatttg atgccctttt tgcacgcttt
      301 cgtaccagaa cctggctcat cagtgatttt ctttgtcata atcattgctg agacaggctc
      361 tgttgagggc gtataatccg aaaagctaat acgcgtttcg atttggtttg cctcgcgatc
      421 gcggggtgaa aatgtttgta gaaaacttct gacaggttgg tttatgagtg cgaataccga
      481 agctcaagga agcgggcgcg gcctggaagc gatgaagtgg gtcgttgtgg tggcattgct
      541 cctggtggcg attgtcggca actaccttta tcgcgacatt atgctgccgc tgcgtgcgct
      601 ggccgtagta attctgattg ctgcagcggg tggtgtcgcg ctgttaacga caaaaggtaa
      661 agctaccgtt gcttttgccc gtgaagcgcg taccgaagtc cgtaaggtca tttggccgac
      721 tcgccaggaa acattgcaca ccacgctgat tgtggctgcg gttaccgcag taatgtcact
      781 gatcctgtgg ggactggatg gtattctggt tcgcctggta tcctttatca ctggcctgag
      841 gttctgagat gtctgaagct cctaaaaagc gctggtacgt cgttcaggcg ttttccggtt
      901 ttgaaggccg cgtagcaacg tcgctacgtg agcatatcaa attacacaac atggaagatt
      961 tgtttggtga agtcatggta ccaaccgaag aagtggttga aatccgtggc ggtcagcgtc
     1021 gcaaaagcga acgtaaattc ttccctggct acgtcctcgt tcagatggtg atgaacgacg
     1081 cgagctggca cctggtgcgc agcgtaccgc gtgtgatggg cttcatcggc ggtacttccg
     1141 atcgtcctgc gccaatcagc gataaagaag tcgatgcgat tatgaaccgc ctgcagcagg
     1201 ttggtgataa gccgcgtccg aaaacgctgt ttgaaccggg tgaaatggtc cgtgttaatg
     1261 atggtccgtt cgctgacttc aacggtgttg ttgaagaagt ggattacgag aaatctcgtc
     1321 tgaaagtgtc tgtttctatc ttcggtcgtg cgaccccggt agagctggac ttcagccagg
     1381 ttgaaaaagc ctaacccagc gatcaaaaaa gcggcgattt aatcgttgca caaggcgtga
     1441 gattggaata caatttcgcg ccttttgttt ttatgggcct tgcccgtaaa acgatttttt
     1501 atatcacggg gagcctctca gaggcgttat tacccaactt gaggaattta taatggctaa
     1561 gaaagtacaa gcctatgtca agctgcaggt tgcagctggt atggctaacc cgagtccgcc
     1621 agtaggtccg gctctgggtc agcagggcgt aaacatcatg gaattctgca aagcgttcaa
     1681 cgcaaaaact gattccatcg aaaaaggtct gccgattccg gtagtaatca ccgtttacgc
     1741 tgaccgttct ttcactttcg ttaccaagac tccgccggca gcagttctgc tgaaaaaagc
     1801 ggctggtatc aagtctggtt ccggtaagcc gaacaaagac aaagtgggta aaatttcccg
     1861 cgctcagctg caggaaatcg cgcagaccaa agctgccgac atgactggtg ccgacattga
     1921 agcgatgact cgctccatcg aaggtactgc acgttccatg ggcctggtag tggaggacta
     1981 agaaatggct aaactgacca agcgcatgcg tgttatccgc gagaaagttg atgcaaccaa
     2041 acagtacgac atcaacgaag ctatcgcact gctgaaagag ctggcgactg ctaaattcgt
     2101 agaaagcgtg gacgtagctg ttaacctcgg catcgacgct cgtaaatctg accagaacgt
     2161 acgtggtgca actgtactgc cgcacggtac tggccgttcc gttcgcgtag ccgtatttac
     2221 ccaaggtgca aacgctgaag ctgctaaagc tgcaggcgca gaactggtag gtatggaaga
     2281 tctggctgac cagatcaaga aaggcgaaat gaactttgac gttgttattg cttctccgga
     2341 tgcaatgcgc gttgttggcc agctgggcca ggttctgggt ccgcgcggcc tgatgccaaa
     2401 cccgaaagtg ggtactgtaa cgccgaacgt tgctgaagcg gttaaaaacg ctaaagctgg
     2461 ccaggttcgt taccgtaacg acaaaaacgg catcatccac accaccatcg gtaaagtgga
     2521 ctttgacgct gacaaactga aagaaaacct ggaagctctg ctggttgcgc tgaaaaaagc
     2581 aaaaccgact caggcgaaag gcgtgtacat caagaaagtt agcatctcca ccaccatggg
     2641 tgcaggtgtt gcagttgacc aggctggcct gagcgcttct gtaaactaat gcctttacgt
     2701 gggcggtgat tttgtctaca atcttacccc cacatataat gcttaatgca gacgtatatc
     2761 cgagatattc gggttgtggc aaggcggcaa ctgagtgagt cgccaggagc atagctaact
     2821 atgtgactgg tgcgaatgaa ggaagccaac gccgccacaa gttgaatagc gacggataga
     2881 aaagatttgt tcgttggagc ctggcctatc caggcctccg tcgaagaccg caggagtttc
     2941 gcaagaaact taatcccctg cgtagacggt gacagaacgc taagattatt cttttatatt
     3001 ctggcttgtt tctgctcacc gtaattaaga cgctctctcc gcttggagga gtgaagtgag
     3061 ttccagagat tttctctggc aaacatccag gagcaaagct aatggcttta aatcttcaag
     3121 acaaacaagc gattgttgct gaagtcagcg aagtagccaa aggcgcgctg tctgcagtag
     3181 ttgcggattc ccgtggcgta actgtagata aaatgactga actgcgtaaa gcaggtcgcg
     3241 aagctggcgt atacatgcgt gttgttcgta acaccctgct gcgccgtgct gttgaaggta
     3301 ctccgttcga gtgcctgaaa gacgcgtttg ttggtccgac cctgattgca tactctatgg
     3361 aacacccggg cgctgctgct cgtctgttca aagagttcgc gaaagcgaat gcaaaatttg
     3421 aggtcaaagc cgctgccttt gaaggtgagc tgatcccggc gtctcagatc gaccgcctgg
     3481 caactctgcc gacctacgaa gaagcaattg cacgcctgat ggcaaccatg aaagaagctt
     3541 cggctggtaa actggttcgt actctggctg ctgtacgcga tgcgaaagaa gctgcttaat
     3601 cgcagttatc tttttaacgc attcgcttac gtataaactt attctgatat tcaggaacaa
     3661 tttaaatgtc tatcactaaa gatcaaatca ttgaagcagt tgcagctatg tctgtaatgg
     3721 acgttgtaga actgatctct gcaatggaag aaaaattcgg tgtttccgct gctgctgctg
     3781 tagctgtagc tgctggcccg gttgaagctg ctgaagaaaa aactgaattc gacgtaattc
     3841 tgaaagctgc tggcgctaac aaagttgctg ttatcaaagc agtacgtggc gcaactggcc
     3901 tgggtctgaa agaagctaaa gacctggtag aatctgcacc ggctgctctg aaagaaggcg
     3961 tgagcaaaga cgacgcagaa gcactgaaaa aatccctgga agaagctggc gctgaagttg
     4021 aagttaaata agccaaccct tccggttgca gcctgagaaa tcaggctgat ggctggtgac
     4081 tttttggtca ccagcctttt tgcgctgtaa ggcgccagta gcgtttcaca ctgtttgact
     4141 actgctgtgc ctttcaatgc ttgtttctat cgacgactta atatactgcg acaggacgtc
     4201 cgttctgtgt aaatcgcaat gaaatggttt aagcgtgata gcaacaggca ttgcggaaag
     4261 tgttccattt tccggtcaac aaaatagtgt tgcacaaact gtccgctcaa tggacagatg
     4321 ggtcgacttg tcagcgagct gaggaaccct atggtttact cctataccga gaaaaaacgt
     4381 attcgtaagg attttggtaa acgtccacaa gttctggatg taccttatct cctttctatc
     4441 cagcttgact cgtttcagaa atttatcgag caagatcctg aagggcagta tggtctggaa
     4501 gctgctttcc gttccgtatt cccgattcag agctacagcg gtaattccga gctgcaatac
     4561 gtcagctacc gccttggcga accggtgttt gacgtccagg aatgtcaaat ccgtggcgtg
     4621 acctattccg caccgctgcg cgttaaactg cgtctggtga tctatgagcg cgaagcgccg
     4681 gaaggcaccg taaaagacat taaagaacaa gaagtctaca tgggcgaaat tccgctcatg
     4741 acagacaacg gtacctttgt tatcaacggt actgagcgtg ttatcgtttc ccagctgcac
     4801 cgtagtccgg gcgtcttctt tgactccgac aaaggtaaaa cccactcttc gggtaaagtg
     4861 ctgtataacg cgcgcatcat cccttaccgt ggttcctggc tggacttcga attcgatccg
     4921 aaggacaacc tgttcgtacg tatcgaccgt cgccgtaaac tgcctgcgac catcattctg
     4981 cgcgccctga actacaccac agagcagatc ctcgacctgt tctttgaaaa agttatcttt
     5041 gaaatccgtg ataacaagct gcagatggag ctggtgccgg aacgcctgcg tggtgaaact
     5101 gcatctttcg acatcgaagc taacggtaaa gtgtacgtag aaaaaggccg ccgtatcact
     5161 gcgcgccaca ttcgccagct ggaaaaagac gacgtcaaac tgatcgaagt cccggttgag
     5221 tacatcgcag gtaaagtggt tgctaaagac tatattgatg agtctaccgg cgagctgatc
     5281 tgcgcagcga acatggagct gagcctggat ctgttggcta agctgagcca gtctggtcac
     5341 aagcgtattg aaacgctgtt caccaacgac ctggatcacg gcccgtatat ctctgaaacc
     5401 ttacgtgtcg acccaactaa cgaccgtctg agcgcactgg tagaaatcta ccgcatgatg
     5461 cgccctggcg agccgccgac tcgtgaagca gctgaaagcc tgttcgagaa cctgttcttc
     5521 tccgaagacc gttatgacct gtctgcggtt ggtcgtatga agttcaaccg ttctctgctg
     5581 cgcgaagaaa tcgaaggttc cggtatcctg agcaaagacg acatcattga tgttatgaaa
     5641 aagctcatcg atatccgtaa cggtaaaggc gaagtcgatg atatcgacca cctcggcaac
     5701 cgtcgtatcc gttctgttgg cgaaatggcg gaaaaccagt tccgcgttgg cctggtacgt
     5761 gtagagcgtg cggtgaaaga gcgtctgtct ctgggcgatc tggataccct gatgcctcag
     5821 gacatgatca acgccaagcc gatttccgca gcagtgaaag agttcttcgg ttccagccag
     5881 ctgtctcagt ttatggacca gaacaacccg ctgtctgaga ttacgcacaa acgtcgtatc
     5941 tccgcactcg gcccgggtgg tctgacccgt gaacgtgcag gcttcgaagt tcgagacgta
     6001 cacccgactc attacggtcg cgtatgtcca atcgaaaccc ctgaaggtcc gaacatcggt
     6061 ctgatcaact ctttgtccgt gtacgcacag actaacgaat acggcttcct tgagactccg
     6121 tatcgtaagg tgaccgacgg tgttgtaact gacgaaattc actacctttc tgctatcgaa
     6181 gaaggcaact acgttatcgc ccaggcgaac tccaacctgg atgaagaagg ccacttcgta
     6241 gaagacctgg taacttgccg tagcaaaggc gaatccagct tgttcagccg tgaccaggtt
     6301 gactacatgg acgtatccac ccagcaggtg gtatccgtcg gtgcgtccct gatcccgttc
     6361 ctggaacacg atgacgccaa ccgtgcattg atgggtgcga acatgcaacg tcaggccgtt
     6421 ccgactctgc gcgctgataa gccgctggtt ggtactggta tggaacgtgc tgttgccgtt
     6481 gactccggtg taactgcggt agctaaacgt ggtggtgtcg ttcagtacgt ggatgcttcc
     6541 cgtatcgtta tcaaagttaa cgaagacgag atgtatccgg gtgaagcagg tatcgacatc
     6601 tacaacctga ccaaatacac ccgttctaac cagaacacct gtatcaacca gatgccgtgt
     6661 gtgtctctgg gtgaaccggt tgaacgtggc gacgtgctgg cagacggtcc gtccaccgac
     6721 ctcggtgaac tggcgcttgg tcagaacatg cgcgtagcgt tcatgccgtg gaatggttac
     6781 aacttcgaag actccatcct cgtatccgag cgtgttgttc aggaagaccg tttcaccacc
     6841 atccacattc aggaactggc gtgtgtgtcc cgtgacacca agctggggcc ggaagagatc
     6901 accgctgaca tcccgaacgt gggtgaagct gcgctctcca aactggatga atccggtatc
     6961 gtttacattg gtgcggaagt gaccggtggc gacattctgg ttggtaaggt aacgccgaaa
     7021 ggtgaaactc agctgacccc agaagaaaaa ctgctgcgtg cgatcttcgg tgagaaagcc
     7081 tctgacgtta aagactcttc tctgcgcgta ccaaacggtg tatccggtac ggttatcgac
     7141 gttcaggtct ttactcgcga tggcgtagaa aaagacaaac gtgcgctgga aatcgaagaa
     7201 atgcagctca aacaggcgaa gaaagacctg tctgaagaac tgcagatcct cgaagcgggt
     7261 ttgttcagcc gtatccgtgc tgtgctggta gccggtggcg ttgaagctga gaagctcgac
     7321 aaactgccgc gcgatcgctg gctggagctg ggcctgaccg acgaagagaa acaaaatcag
     7381 ctggaacagc tggctgagca gtatgacgaa ctgaaacacg agttcgagaa gaaactcgaa
     7441 gcgaaacgcc gcaaaatcac ccagggcgac gatctggcac cgggcgtgct gaagattgtt
     7501 aaggtgtatc tggcggttaa acgccgtatc cagcctggtg acaagatggc aggtcgtcac
     7561 ggtaacaagg gtgtaatttc taagatcaac ccgatcgaag atatgcctta cgatgaaaac
     7621 ggtacgccgg tagacatcgt actgaacccg ctgggcgtac cgtctcgtat gaacatcggt
     7681 cagatcctcg aaacccacct gggtatggct gcgaaaggta tcggcgacaa gatcaacgcc
     7741 atgctgaaac agcagcaaga agtcgcgaaa ctgcgcgaat tcatccagcg tgcgtacgat
     7801 ctgggcgctg acgttcgtca gaaagttgac ctgagtacct tcagcgatga agaagttatg
     7861 cgtctggctg aaaacctgcg caaaggtatg ccaatcgcaa cgccggtgtt cgacggtgcg
     7921 aaagaagcag aaattaaaga gctgctgaaa cttggcgacc tgccgacttc cggtcagatc
     7981 cgcctgtacg atggtcgcac tggtgaacag ttcgagcgtc cggtaaccgt tggttacatg
     8041 tacatgctga aactgaacca cctggtcgac gacaagatgc acgcgcgttc caccggttct
     8101 tacagcctgg ttactcagca gccgctgggt ggtaaggcac agttcggtgg tcagcgtttc
     8161 ggggagatgg aagtgtgggc gctggaagca tacggcgcag catacaccct gcaggaaatg
     8221 ctcaccgtta agtctgatga cgtgaacggt cgtaccaaga tgtataaaaa catcgtggac
     8281 ggcaaccatc agatggagcc gggcatgcca gaatccttca acgtattgtt gaaagagatt
     8341 cgttcgctgg gtatcaacat cgaactggaa gacgagtaat tctcgctcaa acaggtcact
     8401 gctgtcgggg taaaacccgg cagcggattg tgctaactcc gacgggagca aatccgtgaa
     8461 agatttatta aagtttctga aagcgcagac taaaaccgaa gagtttgatg cgatcaaaat
     8521 tgctctggct tcgccagaca tgatccgttc atggtctttc ggtgaagtta aaaagccgga
     8581 aaccatcaac taccgtacgt tcaaaccaga acgtgacggc cttttctgcg cccgtatctt
     8641 tgggccggta aaagattacg agtgcctgtg cggtaagtac aagcgcctga aacaccgtgg
     8701 cgtcatctgt gagaagtgcg gcgttgaagt gacccagact aaagtacgcc gtgagcgtat
     8761 gggccacatc gaactggctt ccccgactgc gcacatctgg ttcctgaaat cgctgccgtc
     8821 ccgtatcggt ctgctgctcg atatgccgct gcgcgatatc gaacgcgtac tgtactttga
     8881 atcctatgtg gttatcgaag gcggtatgac caacctggaa cgtcagcaga tcctgactga
     8941 agagcagtat ctggacgcgc tggaagagtt cggtgacgaa ttcgacgcga agatgggtgc
     9001 ggaagcaatc caggctctgc tgaagagcat ggatctggag caagagtgcg aacagctgcg
     9061 tgaagagctg aacgaaacca actccgaaac caagcgtaaa aagctgacca agcgtatcaa
     9121 actgctggaa gcgttcgttc agtctggtaa caaaccagag tggatgatcc tgaccgttct
     9181 gccggtactg ccgccagatc tgcgtccact ggttccgctg gatggtggtc gtttcgcgac
     9241 ttctgacctg aacgatctgt atcgtcgcgt cattaaccgt aacaaccgtc tgaaacgtct
     9301 gctggatctg gctgcgccgg acatcatcgt acgtaacgaa aaacgtatgc tgcaggaagc
     9361 ggtagacgcc ctgctggata acggtcgtcg cggtcgtgcg atcaccggtt ctaacaagcg
     9421 tcctctgaaa tctttggccg acatgatcaa aggtaaacag ggtcgtttcc gtcagaacct
     9481 gctcggtaag cgtgttgact actccggtcg ttctgtaatc accgtaggtc catacctgcg
     9541 tctgcatcag tgcggtctgc cgaagaaaat ggcgctggag ctgttcaaac cgttcatcta
     9601 cggcaagctg gaactgcgtg gtcttgctac caccattaaa gctgcgaaga aaatggttga
     9661 gcgcgaagaa gctgtcgttt gggatatcct ggacgaagtt atccgcgaac acccggtact
     9721 gctgaaccgt gcaccgactc tgcaccgtct gggtatccag gcatttgaac cggtactgat
     9781 cgaaggtaaa gctatccagc tgcacccgct ggtttgtgcg gcatataacg ccgacttcga
     9841 tggtgaccag atggctgttc acgtaccgct gacgctggaa gcccagctgg aagcgcgtgc
     9901 gctgatgatg tctaccaaca acatcctgtc cccggcgaac ggcgaaccaa tcatcgttcc
     9961 gtctcaggac gttgttctgg gtctgtacta tatgacccgt gactgtgtta acgccaaagg
    10021 cgaaggcatg gtgctgactg gcccgaaaga agcagaacgt ctgtatcgct ctggtctggc
    10081 ttctctgcat gcgcgcgtta aagtgcgtat caccgagtat gaaaaagatg caaacggtga
    10141 attagtagcg aaaaccagcc tgaaagacac gactgttggc cgtgccattc tgtggatgat
    10201 tgtaccgaaa ggtctgcctt actccatcgt caaccaggcg ctgggtaaaa aagcaatctc
    10261 caaaatgctg aacacctgct accgcattct cggtctgaaa ccgaccgtta tttttgcgga
    10321 ccagatcatg tacaccggct tcgcctatgc agcgcgttct ggtgcatctg ttggtatcga
    10381 tgacatggtc atcccggaga agaaacacga aatcatctcc gaggcagaag cagaagttgc
    10441 tgaaattcag gagcagttcc agtctggtct ggtaactgcg ggcgaacgct acaacaaagt
    10501 tatcgatatc tgggctgcgg cgaacgatcg tgtatccaaa gcgatgatgg ataacctgca
    10561 aactgaaacc gtgattaacc gtgacggtca ggaagagaag caggtttcct tcaacagcat
    10621 ctacatgatg gccgactccg gtgcgcgtgg ttctgcggca cagattcgtc agcttgctgg
    10681 tatgcgtggt ctgatggcga agccggatgg ctccatcatc gaaacgccaa tcaccgcgaa
    10741 cttccgtgaa ggtctgaacg tactccagta cttcatctcc acccacggtg ctcgtaaagg
    10801 tctggcggat accgcactga aaactgcgaa ctccggttac ctgactcgtc gtctggttga
    10861 cgtggcgcag gacctggtgg ttaccgaaga cgattgtggt acccatgaag gtatcatgat
    10921 gactccggtt atcgagggtg gtgacgttaa agagccgctg cgcgatcgcg tactgggtcg
    10981 tgtaactgct gaagacgttc tgaagccggg tactgctgat atcctcgttc cgcgcaacac
    11041 gctgctgcac gaacagtggt gtgacctgct ggaagagaac tctgtcgacg cggtaaaagt
    11101 acgttctgtt gtatcttgtg acaccgactt tggtgtatgt gcgcactgct acggtcgtga
    11161 cctggcgcgt ggccacatca tcaacaaagg tgaagcaatc ggtgttatcg cggcacagtc
    11221 catcggtgaa ccgggtacac agctgaccat gcgtacgttc cacatcggtg gtgcggcatc
    11281 tcgtgcggct gctgaatcca gcatccaggt gaaaaacaaa ggtagcatca agctcagcaa
    11341 cgtgaagtcg gttgtgaact ccagcggtaa actggttatc acttcccgta acaccgaact
    11401 gaaactgatc gacgaattcg gtcgtaccaa agaaagctac aaagtgcctt acggtgcggt
    11461 actggcgaaa ggcgatggcg aacaggttgc tggcggcgaa accgttgcaa actgggaccc
    11521 gcacaccatg ccggttatca ccgaagtaag cggttttgta cgctttactg acatgatcga
    11581 cggccagacc attactcgtc agaccgacga actgaccggt ctgtcttcgc tggtggttct
    11641 ggattccgca gaacgtaccg caggtggtaa agatctgcgt ccggcactga aaatcgttga
    11701 tgctcagggt aacgacgttc tgatcccagg taccgatatg cctgcgcagt acttcctgcc
    11761 gggtaaagcg attgttcagc tggaagatgg cgtacagatc agctctggtg acaccctggc
    11821 gcgtattccg caggaatccg gcggtaccaa ggacatcacc ggtggtctgc cgcgcgttgc
    11881 ggacctgttc gaagctcgtc gtccgaaaga gccggcaatc ctggctgaaa tcagcggtat
    11941 cgtttccttc ggtaaagaaa ccaaaggtaa acgtcgtctg gttatcaccc cggtagacgg
    12001 tagcgatccg tacgaagaga tgattccgaa atggcgtcag ctcaacgtgt tcgaaggtga
    12061 acgtgtagaa cgtggtgacg taatttccga cggtccggaa gcgccgcacg acattctgcg
    12121 tctgcgtggt gttcatgctg tgactcgtta catcgttaac gaagtacagg acgtataccg
    12181 tctgcagggc gttaagatta acgataaaca catcgaagtt atcgttcgtc agatgctgcg
    12241 taaagctacc atcgttaacg cgggcagctc cgacttcctg gaaggcgaac aggttgaata
    12301 ctctcgcgtc aagatcgcaa accgcgaact ggaagcgaac ggcaaagtgg gtgcaactta
    12361 ctcccgcgat ctgctgggta tcaccaaagc gtctctggca accgagtcct tcatctccgc
    12421 ggcatcgttc caggagacca ctcgcgtgct gaccgaagca gccgttgcgg gcaaacgcga
    12481 cgaactgcgc ggcctgaaag agaacgttat cgtgggtcgt ctgatcccgg caggtaccgg
    12541 ttacgcgtac caccaggatc gtatgcgtcg ccgtgctgcg ggtgaagctc cggctgcacc
    12601 gcaggtgact gcagaagacg catctgccag cctggcagaa ctgctgaacg caggtctggg
    12661 cggttctgat aacgagtaat cgttaatccg caaataacgt aaaaacccgc ttcggcgggt
    12721 ttttttatgg gtgttattca gagggcaaat tcttcacgga aaatattaag aaaggcaggg
    12781 cgaaacgagg aagaagcttt tcgccctgga caacattcct gctgacggca ctaccataaa
    12841 aaaacacaac gctttcaaca accttgcgtt tttcatcttc cgtagcggct cggaataaat
    12901 ttacgttctt acattccgtc tcatagtctt tgcgaggcgc gtcccagata gctgtcccag
    12961 tctttccata ccggctgcaa accctgagcg gttaacgcgg cagcaaccgc ttccggtctg
    13021 cgatcgtcgt gcggtgagaa ctgttccaac tcggggtgat tatcggcata gccacctggc
    13081 tgcgttttcg agaaggcgct gacgttattg atcgccagcg gaataacgcg atcgcgaaac
    13141 cacggtgatt cccgcgtgga gagtgacagt tcaatctccg gtgcaagcag tcggaaggcg
    13201 cagatggttt gcactaactg gcgttcatcc ataatcgacg caggctcaat gccgccagta
    13261 cacgggcgca ggcgcggaaa ggagacagag taacggcttt gccagtaatg ctgttgcagc
    13321 catagcaaat gttcggcaac catatagcag tcaacgcgcc agttgtcgga aaggccaatt
    13381 agcgcgccga ggcctatctt atcaatcccc gcacgcccca gccgatccgg cgtttccagc
    13441 cgccagaaga agtcctgttt tttgcctttc aaatgatggc gggtataagt cgcctcgtga
    13501 tatgtctcct gataaaccat caccccatcc agaccaagtt gctttaactc ggcgtattcc
    13561 gtctccgcca gcggttgcac ttccatttgt agtgaagaga actgttcacg cagggcgggg
    13621 agatgacgac gaaagtaatc catccccact ttcgcctgat gttcaccagt gactaatagc
    13681 agatgttcaa agcccatctc ccgtatagcg gcgctttccc tggcaatatc cgcttcatcc
    13741 agcgttttgc gcttgatgcg attactcatg gaaaatccgc agtacgtgca gtcgttagcg
    13801 caaagattgg aaagataaag cgggacgtag aaactaactg tgttgccaaa tcgctgacgg
    13861 gtcagacgct gcgcccgttg ggccagtggt tccagatagc cactggcggc tggcgataac
    13921 agcgccatca tatcgtcgcg ggtgagttgc gaggcattta gcgcccgttc tacgtcagca
    13981 gccgttttgc cgttgatacg caggcggatg tcgtcccagt ccagttgtcg ccagcgatcg
    14041 ctgaaggttt tcatgccgat gcctccagaa atccagtcag cgggctggtg gcatgagcaa
    14101 aatgactgcg gctgcccggt ccggactgac gtgccagcag gcctgcttct accgccagac
    14161 gaaatgcctt cgccatgttg acgggatcgt ccgctacggc aatcgccgta ttcactaaca
    14221 ccgcgtcggc ccccatttcc agggcctgcg cggcatggct gggaacgccg atgccagcat
    14281 cgacaaccac cggaactgtg gcctgctgga taataatctc cagcatggca cgggtttcca
    14341 gtccctgatt cgagccaatc ggcgcgccga gcggcatcac cgctgcacag ccgacttctt
    14401 ccagacgttt acacaatacc ggatcggccc cgcagtaagg cagcacgaca aatccctgtt
    14461 gtaccagcat ttcggcggct ttcagagttt cgatgggatc gggcaacagc cagcgggcgt
    14521 cagggtgaat ctctaatttt aaccagtttg tgcctaacgc ttcacgtgcc agatgggcgg
    14581 cgaaaatggc ttcttccgct gttttcgccc cggaagtatt tggcagcagg gtcacacccg
    14641 ccgcgataag cggttcgagg atagcgtcgt tgtgctggcg caagtcgaca cgtttcatcg
    14701 ccagtgtcac cagctggctg ccggaagcgc ggatcgcctc caccatcagt tgtgaagaag
    14761 cgaatttccc tgtgccggta aacagatgtg aatcaaacgt tttgtccgca atacgtaaca
    14821 tctcaacccc ctgcaataac ctgaaaaagc aggatctggt cgccatcctg cacgatatgt
    14881 tgcgtccact gctcacgcgg gacgatttgc tgattaatcg ccagagccgc gcccgcttgt
    14941 cgttggtcga gttgctccag tagttcgtga acagtttgcc cggcggcgca ctgcatcggt
    15001 tgatcgttaa acaggatctg cattgcttcc tccgcatacc gggcaaccac tggcgcggcg
    15061 caacgccagg ctgcgccact ggctcgattt accgtcgaac agtcggagtt ctcccgcagg
    15121 tgtctctata ccgcttaata acttaatggc ttccagtgcc tgcaaagtgc ccataacccc
    15181 gaccaccggg ccaaccacgc ccgccgtgcg gcagttgcgt tctggctcct ggttatctgg
    15241 ccacaggcag cggtaacacc cctgctccca gggcggcgtc agtaccatca actgaccgcc
    15301 aaatccgacc gcgctggcgg tgataagcgg cgtgttgagt accacgcagg cggcattaat
    15361 ctcctggcga gtcgccatat tgtcggtaca gtcgagcacc acatcggccc gtgcaaccgc
    15421 atcttttaac gcctcacccg ttaaccgttg ttgtaatgct gtcagttgaa tatcgggatt
    15481 caactgtgtc agtcgctgat ggctgacctg cgatttcggg cgatcgatat cttcagtggt
    15541 aaagaggatt tgtcgttgca gattgcttaa atgcacatcg tcgtcatctg ccagtaccag
    15601 cgtcccgacg ccagcccccg ccaggtatag cgcagcaggt gtacccagcc cgccaagacc
    15661 aataatcagc acctggctgt cgagcagttt ttgctgcccg tccagagcga tatcgtcgag
    15721 cagaatttgg cggctataac gcataaagtc acggtcattc atcgccaact cctgcaattt
    15781 ccagcaactg tgccgttgcc aaacgccagt ctgcggcttg agtaatggcg ctgacgacgg
    15841 cgatactgcc gacacccgtt gctatcaccg caggcgcgcg cgccagactg ataccgccaa
    15901 tcgccacggt gggataatcc gccagtcgct caacatgccg tgccagctgt tccagcccct
    15961 gcggtgcaga aggcatctgt ttggtttgcg tcgggaacac atgtcccagc gcgatataag
    16021 aggggcgtgc tgccagcgcg acgtcgattt ccatatcgtc atgtgtcgaa acgcccagcc
    16081 gcaggcctgc cgcgcggatg gcattgagat cggtggcttg caaatcttcc tgccccaaat
    16141 ggacgccata cgcctgatgc ttgatcgcca gccgccagta atcgttgata aacaatcgcg
    16201 cgttatagcg gcggcccagc gcaattgccg ccacgacatc ggcttccacc tcttcattgc
    16261 gccgatcttt gatgcgtagc tggagggtac gtacgcctgc atccaacaga cgttcgatcc
    16321 actgtacgct gtccaccacc gggtacagtc ctaaacgaaa aggtacagga ggaaaatcag
    16381 gctgatacat cacgcttcct ccttacgcag gtagatttct ccgcctctgg cgcggaagtt
    16441 ctccgacata tccgccattc ccacttcaat ggtttgcgcg gcggcgtaat cacgtacttc
    16501 ctggctgatt ttcatcgagc agaatttcgg cccacacatg gagcaaaaat gggcgacttt
    16561 acctgactct tgcggcaggg tttcatcgtg ataagcgcgg gcggtaaacg ggtcgagggc
    16621 cagattaaac tggtcttccc agcgaaattc gaagcgggct ttcgacatgg cgttatcgcg
    16681 aatttgcgcg cctggatgcc ctttcgccag gtcagcggcg tggacggcaa tcttataggt
    16741 gataagcccc tgcttaacat cttctttatt aggcagaccc agatgctctt ttggcgttac
    16801 gtaacagagc atcgcgcagc caaaccagcc aatcatcgcc gcaccaatcc ccgacgtgaa
    16861 gtggtcatag cccggcgcaa tatcggtagt tagcggcccc agagtgtaaa acggcgcttc
    16921 gtggcagtgc tctaactcct cggtcatatt gcggcggatc atctgcatcg gcacgtggcc
    16981 tgggccttca atcatcacct gcacgtcata ttcccaggca attttggtca gttcgcccag
    17041 cgtatgcagc tcggcaaact gcgcttcatc gttggcgtcc tgaatagaac cggggcgcag
    17101 accgtcgccc agcgacagcg aaacgtcata agcggcacag atttcacaaa tttcgcggaa
    17161 gtgttgatag aggaaatttt cctgatgatg ggagaggcac catttcgcca taatcgaacc
    17221 gccgcgagag acgataccgg tcaggcgttt cgcggtcatc ggcacatagc gcagcagtac
    17281 gcccgcatgg atagtgaagt aatccacacc ttgctcggcc tgttccagca gcgtgtcgcg
    17341 gaacgcttcc caggtaagat cttcggcgat cccgttaacc ttctccagcg cctggtagat
    17401 cggcactgta ccgatcggca ccgggctgtt acgcaaaatc cactcgcggg tttcgtgaat
    17461 atagcgaccg gtggagagat ccatcaccgt atccgctccc cagcgcgtgg accataccag
    17521 cttttccact tcttcttcga tggaagaggt gaccgccgag ttgccgatat tggcgttaac
    17581 ttttaccagg aaattgcgac caataatcat cggctccgat tccggatgat taatgttggc
    17641 cgggataatc gcacgtccgg cagcaacttc atcacggaca aattccgcag tgatattttc
    17701 cggcagatgt gcgccaaagc tcattcccgg atgctggtgg cgtaaaacct cgctacggat
    17761 gcgctcgcgg cccatattct cgcggatggc gatgaattcc atttccggcg tgatgatgcc
    17821 ctggcgggcg tagtgcagtt gggtgacacg gcgtcctgtt ttggcgcgtt ttggcgttaa
    17881 taagccgcta aaacgtagct cgtcgagacc atcatctgcc agccgcgttc tggtgtaatc
    17941 ggaactgcgc acggtaagtt cttcggtatc gccgcgcgca tcgatccacg gctggcgtag
    18001 ttttgccagc ccttgctgca cgttaatggc gatctgcgga tcaccatacg ggccggaggt
    18061 gtcgtagacc ggaatcgctt cgttttcttc gtactgcggc tgttctttgc taccgccaat
    18121 tagcgtcggg ctaagctgga tctcacgcat cggcacgcgc acgccggggt gtgtgccagt
    18181 gatataaatg cgttttgagt tgggaaaggc ggtgccttcc agggtgtcga taaaatgttg
    18241 ggcccgggcg cgttgttcgc ggcgggtcag ttttgttgca gacatagctc attccaaaaa
    18301 gtcaaggacg tggcttgtca gacgacggat gaagcaagag acgatcgccg caaaggcgat
    18361 gcgatagcag attaactctt gttcccttcg caggtattag cctgatcagg ttccgcggat
    18421 cccgaataaa cggtctcagc cagttaaggc actccgacaa gaaattagcc cccacaaacg
    18481 gggcgttgaa tgtaaattac gcgttaacag cgcagaactc aagcaggatg tttgacgcgg
    18541 gcggcgtcaa gcaccagcag aatcagctta tcttccagca caaagcgcgc ttccagcgcc
    18601 tcgccgatgt cagataaaac ctgttgaaac tcaaggtaat tatcatgatc gatggcggtt
    18661 tccaggctgg aatcgtagta atccataatc tgttgggtat tggcttcgag ttgcggccaa
    18721 atcttcgcgg cgcgcgcgag ttgcccgttg ccttccagct tatgaagaat gcgctcataa
    18781 atactgaaat gtccggcaga caagtaatcg accaggctct gacaaaaatc atcaagggct
    18841 ttttcgttta gcctcatgta cgattctttg ccaggcttaa tgccaaccag attgtagtaa
    18901 gccacgagca gatgcttacg tacatgtagc cagcgatcaa ccagtttgtt acttcctctg
    18961 acgcgctccg tcaggttatc gagctggtta agcatgattg actccgcaag tttgtattca
    19021 aaaactgctc agtgagaaat gtaaaaacca tgttaaacat gccagtgatg caaaggtagt
    19081 gcaagagcta tggatcgtat aattgaaaaa ttagatcacg gctggtgggt cgtcagccat
    19141 gaacaaaaat tatggttgcc gaagggagaa ttgccatatg gcgaagcggc aaatttcgat
    19201 cttgtgggtc agcgcgcact acaaatcggc gaatggcagg gggaacctgt ttggttagta
    19261 caacagcagc ggcgtcacga tatggggtcg gtacgtcagg tcattgatct cgatgttggg
    19321 ctgtttcaac tggccggacg aggcgtacaa ctggcggagt tttaccgatc gcataaatac
    19381 tgtggttact gcgggcatga aatgtatccg agcaaaaccg aatgggcgat gctgtgcagc
    19441 cattgccgtg agcgttacta cccgcaaatc gccccctgca ttattgttgc catccgtcgc
    19501 gatgattcga tcctcctcgc ccagcatacc cgccatcgta acggtgtcca tacagtactt
    19561 gccggattcg tcgaagtggg cgaaaccctc gagcaggcag tcgcgcggga agtgatggaa
    19621 gagagcggaa ttaaagttaa aaacttgcgt tacgtgactt ctcagccgtg gccgtttcct
    19681 cagtctttaa tgaccgcgtt tatggcggaa tatgacagcg gcgacatcgt gatcgacccg
    19741 aaagaattgc tcgaggcgaa ctggtatcgc tatgacgatt tgccgttact cccgccgccc
    19801 ggcaccgtag cgcgccgtct gatagaagat actgtggcga tgtgtcgggc agagtatgag
    19861 tgatgataca ctgaccgcct gacgcactaa ggaacagcca aaatgaccga acttaaaaac
    19921 gatcgttatc tgcgggcgct gctgcgccag cccgttgatg tcactccagt atggatgatg
    19981 cgccaggcgg gtcgctatct accggaatat aaagccacgc gcgcccaggc gggcgatttt
    20041 atgtcgctgt gcaaaaacgc cgagctggcg tgcgaagtga ctttgcaacc gctgcgtcgc
    20101 tacccgctgg atgcggcgat cctcttttcc gatatcctca ccgtgccgga cgcgatgggg
    20161 ttagggctct attttgaagc cggagaaggt ccgcgtttta cctcgccagt cacctgcaaa
    20221 gccgacgtcg ataaactgcc aattccggac ccggaagatg agctgggtta cgtgatgaac
    20281 gcggtgcgta ccattcgtcg cgaactgaaa ggcgaagtgc cgctgattgg tttttccggc
    20341 agcccgtgga cgctggcgac ctacatggtg gaaggcggca gcagcaaagc gttcaccgtg
    20401 atcaaaaaaa tgatgtatgc cgatccgcag gcgctgcacg ctctactcga taaactggcg
    20461 aaaagcgtca ctttgtatct gaatgcgcag attaaagccg gtgctcaggc agtgatgatt
    20521 ttcgacacct ggggcggtgt gcttaccggg cgcgattatc aacagttctc gctctattac
    20581 atgcataaaa ttgttgatgg tttactgcgt gaaaacgacg gtcgccgcgt gccagtcacg
    20641 ctgtttacca aaggcggcgg acagtggctg gaagcgatgg cagaaaccgg ttgcgatgcg
    20701 ttgggcctcg actggacaac ggatatcgcc gatgcgcgcc gccgtgtggg caataaagtc
    20761 gcgttgcagg gtaatatgga tccgtcgatg ctgtacgcgc cgcctgcccg cattgaagaa
    20821 gaagtagcga ctatacttgc aggtttcggt cacggcgaag gtcatgtctt taaccttggt
    20881 cacggcattc atcaggatgt gccgccagaa catgctggcg tgttcgtgga ggcagtgcat
    20941 cgactgtctg aacaatatca ccgctaagga gtgattatgg atctcgcgtc attacgcgct
    21001 caacaaatcg aactggcttc ttctgtgatc cgcgaggatc gactcgataa agatccaccg
    21061 gatctgatcg ccggagtcga tgtcgggttt gagcagggcg gagaagtgac gcgagcggcg
    21121 atggtgctgc tgaaatatcc ctcgcttgag ctggtggagt ataaagttgc ccgcatcgcc
    21181 accaccatgc cttacattcc aggttttctt tccttccgcg aatatcctgc gctgctggca
    21241 gcgtgggaga tgctgtcgca aaagccggat ttagtgtttg tcgatggtca tgggatctcg
    21301 catcctcgcc gtcttggcgt cgctagccat tttggtttga tggtggatgt gccaacgatc
    21361 ggcgtggcga aaaaacggct ctgcggtaaa ttcgaaccgc tctccagcga accgggcgcg
    21421 ctggccccac tgatggataa aggcgagcag ctggcctggg tctggcgcag caaagcgcgc
    21481 tgtaacccgt tgtttatcgc taccggccat cgggtcagcg tggacagcgc gctggcgtgg
    21541 gtacaacgct gcatgaaagg ctatcgtctg ccggagccaa cgcgctgggc ggacgcggtg
    21601 gcctcggaac gtccggcgtt cgtgcgctat acagcaaatc agccctaatt caggtaaact
    21661 gcggataatt tccgtatttg agaactcaac atgttacaaa acccaattca tctgcgtctg
    21721 gagcgcctgg aaagctggca gcatgtcact ttcatggctt gcttatgcga acgcatgtac
    21781 cccaattacg ccatgttctg ccagcaaacc ggttttggtg atgggcaaat ttaccgtcgt
    21841 attctcgatc tcatctggga aacgctgacc gttaaagacg caaaagtaaa tttcgacagc
    21901 caactggaga aatttgaaga agcgattcct tcagccgacg atttcgatct gtacggcgtt
    21961 tatccggcaa tcgatgcctg cgtggcgtta agtgaactgg tccattcgcg tttgagtggt
    22021 gaaacgctcg aacacgcggt ggaagtgagt aagacctcca tcacgaccgt tgcgatgctg
    22081 gaaatgactc aggctggtcg cgaaatgagc gatgaagagc ttaaagaaaa cccagctgta
    22141 gagcaagaat gggacattca gtgggaaata ttccgacttt tagctgagtg cgaagaacgc
    22201 gatatcgagc tgataaaagg ccttagggca gacctgcgtg aggcgggtga gagcaatatt
    22261 ggtataattt ttcagcaata agaccagaaa acgtgattta acgcctgatt tgtcgtacct
    22321 ggagtcttcc ctttcgcccc ccgtctggtc tacatttggg gggcgaaaaa aagtggctat
    22381 cggtgcgtgt atgcaggaga gtgctattct ggcatttccg tcgcactcga tgcttagcaa
    22441 gcgataaaca cattgtaagg ataacttatg aacaagactc aactgattga tgtaattgca
    22501 gagaaagcag aactgtccaa aactcaggct aaagctgctc tggagtccac tctggctgca
    22561 attactgagt ctctgaaaga aggcgatgct gtacaactgg ttggtttcgg taccttcaaa
    22621 gtgaaccacc gcgctgagcg tactggccgc aacccgcaga ccggtaaaga aatcaaaatt
    22681 gccgcagcta acgtaccggc atttgtttct ggcaaggcac tgaaagacgc agttaagtaa
    22741 gattgcgtgg cagtgaacag ttttaacgaa ggggtggttt cacccctttt gtctttctgg
    22801 cgtcgatcat tgatgctggc tggcgctctg cttctcactg cctgtagtca taactcttca
    22861 cttcctccct ttaccgccag tggatttgct gaagaccagg gcgcggtacg catctggcga
    22921 aaagacagcg gcgataatgt gcatctgctt gccgtgttta gcccgtggcg cagtggcgat
    22981 accacgacgc gagagtatcg ctggcagggc gataacctca cgctcatcaa tatcaatgtt
    23041 tacagcaaac cgccggtgaa tattcgcgcg cgttttgacg atcgcggtga tctgagcttt
    23101 atgcaacgtg aatccgatgg ggaaaagcag cagctttcta acgaccaaat cgatttatac
    23161 cgttatcgtg ctgatcagat ccgccagatt agcgatgcct tacgtcaggg gagagtcgtg
    23221 ctgcgccagg ggcgctggca tgcgatggaa cagaccgtga ccacctgcga agggcaaacc
    23281 attaaacctg atttagattc gcaggcgata gcgcatatcg agcgccgcca gagccgctct
    23341 tctgttgatg tcagcgtggc atggctggaa gcgcccgaag gttcgcaatt actgttagtg
    23401 gcaaactctg atttctgtcg ctggcaaccc aacgagaaaa cgttctgatt taccagtggc
    23461 ccatacccat atgaccgcca ccaccgcagc cgccgtagcc catgcccatt ccggctccgc
    23521 gcggaatacc cgcttcagcc atcgcgatat ctcgtttcac ccgtaactca tctaacgact
    23581 gacgcaaatt ctccatctct ttggcgaccg cgttaatttt gctgctatcc ggtgggttcg
    23641 cggctaacag ggcattgtat tcataacgct tcgtcaccag ttgctgttgc agtgcgctgc
    23701 tttgagcgta aaagtcatta tggattttct gccacgctgt ctgttgttcg ctggtcaaag
    23761 gcgcggcatt ttgctgccac ataccgtgtc cgccgtgagc aaatgcagat gtcgatccca
    23821 tcgccattgc tgaaagcgcc atcattacca gggcaatttt cgtgttccgt ttcatggtta
    23881 atcctccagt ggttgtctta cttcgggtat tgcatcttcc gtgccaacga tgaaacgctg
    23941 atatgacggg taatctggca tgataaacga gtaaaaatga ctcgcctgct gcgggtagcg
    24001 agtcattttt actcattgaa acttaagcct ttgtgttaca gcgcagggta agcgctgata
    24061 aaagatggca tgatttctgc tgtcagaaag ggatgagcag gcaaagaaga agatgcgttt
    24121 tatgcaacgt tctaaagact ccttagctaa atggttaagc gcgatcctcc ccgtggtcat
    24181 tgttgggctg gtagggttgt ttgcggtgac ggtgattcgc gattatgggc gcgagactgc
    24241 cgccgccaga caaacgctgc tggaaaaagg cagtgtactt attcgcgctc ttgaatccgg
    24301 ctcgcgcgtc ggcatgggga tgcgcatgca tcatgcgcag cagcaggcct tactggaaga
    24361 aatggccggg cagcctggag tacgttggtt tgcggtcacg gatgaacaag gaacaatcgt
    24421 gatgcatagc aactccggca tggtgggaaa acagctttat tccccgcagg aaatgcagca
    24481 gttacatccg ggagatgaag aagcgtggcg gcggatcgat agcgcagacg gcgagcctgt
    24541 tctggaaatt tatcgccagt ttcaaccgat gtttgctgct ggaatgcacc ggatgcgcca
    24601 tatgcagcag tatgccgcga caccacaagc aattttcatc gctttcgacg ccagtaatat
    24661 tgtgagtgcc gaagatcgtg agcagagaaa caccctgatt atcctcttcg ccctggcgac
    24721 ggtcttgctg gcaagcgtat tgtcattctt ctggtatcgc cgctatttgc gctcgcgcca
    24781 gcttctacaa gatgaaatga agcgcaaaga gaagctggtg gcgctggggc atcttgcggc
    24841 aggcgttgcc cacgaaatcc gtaacccact ttcctcgatt aaaggactgg cgaaatactt
    24901 tgccgagcgc gcgcctgcag ggggagaagc gcatcaactg gcgcaggtga tggcgaaaga
    24961 ggccgaccgt ttaaaccgcg tggtaagcga gctgctggaa ctggttaagc caacgcatct
    25021 ggctttgcag gcggtggatc tcaacacgct gattaaccac tcattacagc tggtaagcca
    25081 ggatgcgaac agccgggaga tccagttacg ctttaccgcc aacgacacat taccggaaat
    25141 tcaggccgat ccggacaggc tgactcaggt cctgttgaat ctctatctca atgctattca
    25201 ggcgattggt cagcatggcg tgattagcgt gacggccagc gaaagcggcg cgggcgtgaa
    25261 aatcagcgtt accgacagcg gtaagggaat tgcggcagat cagcttgatg ccatcttcac
    25321 tccgtacttc accactaaag ccgaaggcac cggactgggg ctggcggtcg tgcataatat
    25381 tgttgaacaa cacggtggta caattcaggt cgcaagccag gagggaaaag gcgcaacgtt
    25441 caccctctgg cttccggtca ctattacgcg taagggccca caaggatgac gcacgataat
    25501 atcgatattc tggtggtgga tgatgacatt agccactgca ctattttgca ggctttactg
    25561 cgcggctggg gctataacgt cgcgctggcg aacagcgggc gacaggcgct ggagcaggtg
    25621 cgggaacagg tttttgatct tgtgctttgc gatgtgcgaa tggcggagat ggacggcatt
    25681 gccacgctaa aagagatcaa agcgttaaat ccggcgattc cggtgctgat tatgaccgca
    25741 tactccagcg tcgagacggc ggtagaagcg ttgaaaaccg gggcgctgga ttatctcatc
    25801 aagccgctgg atttcgataa cctgcaggcg acgctggaaa aggcgctcgc gcatacgcac
    25861 agtattgacg ctgaaacgcc tgcggtgtct gccagccagt tcggtatggt cggtaaaagc
    25921 ccggcgatgc aacacctgct cagtgaaatc gccctcgtcg cgccatcgga agccacggtg
    25981 ctgatccacg gcgattccgg caccggtaaa gagctggtcg ccagggcgat tcacgccagt
    26041 agcgcacgta gcgaaaaacc gctggtaacg ctcaactgtg cggcactcaa cgaatccttg
    26101 ctggaatctg aattgttcgg tcacgaaaaa ggggcgttca ctggagccga tagacgccgg
    26161 gaagggcgct ttgttgaggc ggacggcggc acgctgtttc tcgatgaaat tggcgatatc
    26221 tcgccgatga tgcaggtgcg tctgctgcgt gcgattcagg agcgcgaagt tcagcgtgtc
    26281 ggtagcaacc agactatctc ggttgatgtc cggttgattg cggcgaccca tcgcgatctt
    26341 gccgcagagg tgaatgccgg gcgttttcgc caggatctct actatcgcct gaatgtggtg
    26401 gcgattgaag taccatcgct gcggcaacgg cgggaagata tccctctgct ggctgtccat
    26461 tttcttcagc gctttgccga gcgtaatcgc aaggcggtaa aaggctttac gccacaggcg
    26521 atggatttgc tgatccacta cgactggccg ggaaatattc gtgaactgga aaacgcggtg
    26581 gaacgggcgg tggtgctgct gaccggggaa tatatttccg aacgcgagct gccgctggcg
    26641 attgccggta cgccgatccc actggggcaa agtcaggata ttcagccgct ggtggaagtc
    26701 gaaaaagaag tgattctggc ggcactggag aaaacgggcg gcaacaaaac cgaagccgcc
    26761 cgtcagttag ggatcacgcg caaaacgcta ttggcaaaac tgtcgcgtta gttctgctcg
    26821 cgttcgatag cgcgccagcc gatatctttc cggcagaagc agtcgtccca gtgaatatcg
    26881 gtcattaagg cataagcgcg tttctgcgct tctgccacgg tatgacccag cgcggtgacg
    26941 cacagtacgc gcccgccgct ggtcactacc tgctcgttat ccgccagttt cgtgcccgcg
    27001 tggaacactt tgccgcctgc cacttcttcc agcggcaggc cgtggatcac atcgccggtg
    27061 cggtaatcgc ccggatatcc acccgcagcc atcaccacgc cgagagaagc acgctcatcc
    27121 cactctgacg ttttctcgtc cagtttgcct tcacaggccg ccaggcagag ctcaaccaga
    27181 tcggacttca tgcgcagcat aatcggctgg gtttctggat cgccaaagcg gcagttaaat
    27241 tcgataacct tcggattgcc ctgtttgtcg atcatcaggc ccgcgtagag aaaaccggtg
    27301 taggtgttgc cttccgccgc catgcctttc acggttggcc agatgatacg ttccatggtg
    27361 cgctgatgaa cttcatcggt tactaccggc gcgggggagt aagcgcccat cccgcccgtg
    27421 tttggtccgg tatctttatc gcctacgcgt ttgtgatcct ggctggtggc catcggcagc
    27481 acatgctcgc cgtctaccat cacgataaag ctcgcttctt cgccgtcgag gaactcttcg
    27541 ataacgatgc gatgacccgc gtcgccaaaa gcgttgcccg ccagcatatc gtgaacagcc
    27601 gcttcggctt cttccagcgt catcgccacg ataacgcctt tcccggctgc cagaccgtcc
    27661 gctttaatga cgatcggcgc acctttctca cgcagatacg ccagcgcagg ttctacctcg
    27721 gtgaagttct ggtattccgc tgtagggatc ttatggcggg ccaggaaatc tttggtaaac
    27781 gctttcgaac cttccagttg cgcggcacct gcggttgggc cgaagatttt catcccggcg
    27841 gcgcggaagg tatcgaccac gcctttcacc agcggcgctt ccgggccgac gatggtcaga
    27901 tcaatctttt cgttttgtgc gaaatccaac agcgccggga tatcggtcac gccgatagca
    27961 acgttttgca gcgcgggttc cagcgcagtg cctgcattac ccggagcaac aaaaacagtt
    28021 tcaaccagcg gcgactgcgc cgctttccag gccagcgcgt gctcgcgccc gccgttacca
    28081 atcactaata ctttcatcta ttgctccatt aatggcggaa gtggcgcatg tcggtgaaga
    28141 gcatcgcaat accgtgctcg tcggcggcgg caatcacttc gtcatcacgg atagaaccgc
    28201 caggctggat tacgcaggtc acgcctgcag cggcggcggc atcaataccg tcgcggaacg
    28261 ggaagaacgc gtcagaagcc atcgaggaac ctttcacttc caagccttca tcggcagctt
    28321 taataccggc gattttcgcg gagtacacgc ggctcatctg gcccgcgcca atcccgatgg
    28381 tcatattgtt tttggcatag acgatagcgt tggatttcac gaacttcgcc actttccagc
    28441 agaacagcgc atcacgcagt tcctgttcgg tcggctgacg ttgggtgacg acgcgcagtt
    28501 cttccgcacc gaccatgccc aggtcacgat cctgaaccag cagaccgccg ttcacgcgtt
    28561 tgaaatcaag acccggaaca cgctcgcccc actgaccgca ggtcagaacg cgtacgttct
    28621 gcttggtggc ggtgattttc agggcttctt cgctggcgga aggcgcaata atcacttcga
    28681 caaactgacg agaaatgatg gcctgtgcgg tttccgcatc cagctcgcgg ttaaaggcaa
    28741 tgatgccgcc gaatgcggag gttgggtcgg ttttgtacgc gcgatcgtaa gcatcaagaa
    28801 tggaattgcc gatagccacg ccgcaagggt tggcgtgctt cacaatcaca catgccggct
    28861 cggcgaactc tttcacgcac tccagcgccg catcggtatc ggcgatgttg ttataagaga
    28921 gggctttacc ctgaacctgg gttgcggtag caacggaggc ttctttcaca ttctcttcta
    28981 tatagaaggc agcctgctgg tggctgttct cgccgtaacg catatcctgc ttcttaatga
    29041 agttcaggtt cagcgtgcgt gggaagcgac cggcggcttc tttgctttca ccgtggtaag
    29101 ccggaaccat gctgccgaag tagttggcaa tcatgctgtc gtaggcggca gtgtgttcga
    29161 aggctttgat ggcgaggtcg aaacgggttg caagcgtcag cgatccttcg ttgtcatcca
    29221 tctctttaat aatggcgtcg tagtcgctgc tcttcaccac gattgcgaca tctttatggt
    29281 tcttagcggc ggagcgcacc atggtcgggc cgccgatatc gatgttctca accgcatctt
    29341 ccagcgagca gccttcacga gcaacagtct gggcgaacgg atacaggtta acaaccacca
    29401 tatcgatagg ctggatctga tgttcttcca taatggcatc gtcctggccg cgacggccca
    29461 gaatgccacc atgtactttc ggatgcaggg tcttcacgcg tccatccatc atctccggga
    29521 aaccggtgta atcggaaact tcggttaccg gcagaccttt ttctgctaac agacgggcag
    29581 tgccccctgt agacagcagc tccacaccgc gtgcggaaag tgcctgggcg aattcgacga
    29641 taccggcttt gtcagaaaca ctgagcagag cgcggcggac tggacgacgt tgttgcatgg
    29701 taaatcccct ggatttgact attacagaga gcgttagctg aatttttcgc gaaaaactca
    29761 gctaacgccc ctaacggggc atccttattt ttcgcccgca ttgtaacgaa aacgtttgcg
    29821 caacgctcgc gaatttttct ctttcaatgg tgatcacaat tttgacagtg gttaccgtgg
    29881 gaaaaataca gaaattacat tgatgattgt ggataactct gtgcgtaaaa aggtataaag
    29941 cggggttttg ctggggaatg cagcagtcag tcatttttct gcaatttttc tattgcggcc
    30001 tgcggagaaa tccctataat gcgcctccat cgacacggcg gatgtgaatc acttcacaca
    30061 aacagccggt tcggttgaag agaaaaatcc tgaaattcag ggttgactct gaaagaggaa
    30121 agcgtaatat acgccacctc gcgacagtgc gctaaag
//
LOCUS       DAEEKQ010000030        30001 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.030, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000030 DAEEKQ010000000
VERSION     DAEEKQ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.030"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            38..580
                     /locus_tag="KLK57_001380"
     CDS             38..580
                     /locus_tag="KLK57_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0891790.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            663..1364
                     /locus_tag="KLK57_001381"
     CDS             663..1364
                     /locus_tag="KLK57_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBE0891791.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAQNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1389..3988
                     /locus_tag="KLK57_001382"
                     /pseudo
     CDS             1389..3988
                     /locus_tag="KLK57_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3979..5049
                     /locus_tag="KLK57_001383"
     CDS             3979..5049
                     /locus_tag="KLK57_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0891792.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDAFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5061..5603
                     /locus_tag="KLK57_001384"
     CDS             5061..5603
                     /locus_tag="KLK57_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0891793.1"
                     /translation="MKKETIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAASITGADVLFSGEADTLTP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5611..6126
                     /locus_tag="KLK57_001385"
     CDS             5611..6126
                     /locus_tag="KLK57_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0891794.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6092..6829
                     /locus_tag="KLK57_001386"
     CDS             6092..6829
                     /locus_tag="KLK57_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBE0891795.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6940..7950
                     /gene="pyrD"
                     /locus_tag="KLK57_001387"
     CDS             6940..7950
                     /gene="pyrD"
                     /locus_tag="KLK57_001387"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HBE0891796.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8124..8666
                     /gene="zapC"
                     /locus_tag="KLK57_001388"
     CDS             8124..8666
                     /gene="zapC"
                     /locus_tag="KLK57_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HBE0891797.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="KLK57_001389"
     CDS             complement(8663..9772)
                     /gene="ycbX"
                     /locus_tag="KLK57_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HBE0891798.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDRWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVMPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10016..12124
                     /gene="rlmKL"
                     /locus_tag="KLK57_001390"
     CDS             10016..12124
                     /gene="rlmKL"
                     /locus_tag="KLK57_001390"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HBE0891799.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRTGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWVQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHMALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            12136..14043
                     /locus_tag="KLK57_001391"
     CDS             12136..14043
                     /locus_tag="KLK57_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0891800.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQEPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLLEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVDGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLDQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14173..15426
                     /gene="pqiA"
                     /locus_tag="KLK57_001392"
     CDS             14173..15426
                     /gene="pqiA"
                     /locus_tag="KLK57_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HBE0891801.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSQQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15431..17071
                     /gene="pqiB"
                     /locus_tag="KLK57_001393"
     CDS             15431..17071
                     /gene="pqiB"
                     /locus_tag="KLK57_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HBE0891802.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGLE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKVRLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17068..17631
                     /gene="pqiC"
                     /locus_tag="KLK57_001394"
     CDS             17068..17631
                     /gene="pqiC"
                     /locus_tag="KLK57_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HBE0891803.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLASVWSQEAASIAQEIKRLP"
     gene            17887..18054
                     /gene="rmf"
                     /locus_tag="KLK57_001395"
     CDS             17887..18054
                     /gene="rmf"
                     /locus_tag="KLK57_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HBE0891804.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="KLK57_001396"
     CDS             complement(18124..18642)
                     /gene="fabA"
                     /locus_tag="KLK57_001396"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HBE0891805.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18711..20471)
                     /locus_tag="KLK57_001397"
     CDS             complement(18711..20471)
                     /locus_tag="KLK57_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HBE0891806.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20657..21109
                     /gene="matP"
                     /locus_tag="KLK57_001398"
     CDS             20657..21109
                     /gene="matP"
                     /locus_tag="KLK57_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HBE0891807.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="KLK57_001399"
     CDS             complement(21185..22225)
                     /gene="ompA"
                     /locus_tag="KLK57_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HBE0891808.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="KLK57_001400"
     CDS             complement(22580..23089)
                     /gene="sulA"
                     /locus_tag="KLK57_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HBE0891809.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23308..23937
                     /gene="sxy"
                     /locus_tag="KLK57_001401"
     CDS             23308..23937
                     /gene="sxy"
                     /locus_tag="KLK57_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HBE0891810.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="KLK57_001402"
     CDS             complement(23900..26062)
                     /gene="yccS"
                     /locus_tag="KLK57_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HBE0891811.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26072..26518)
                     /locus_tag="KLK57_001403"
     CDS             complement(26072..26518)
                     /locus_tag="KLK57_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HBE0891812.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26641..28695
                     /gene="helD"
                     /locus_tag="KLK57_001404"
     CDS             26641..28695
                     /gene="helD"
                     /locus_tag="KLK57_001404"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HBE0891813.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGERCDLDTTYRFNSRI
                     GKVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="KLK57_001405"
     CDS             complement(28727..29185)
                     /gene="mgsA"
                     /locus_tag="KLK57_001405"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HBE0891814.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29281..29943)
                     /locus_tag="KLK57_001406"
     CDS             complement(29281..29943)
                     /locus_tag="KLK57_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HBE0891815.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
BASE COUNT         7730 a         7086 c         7880 g         7305 t
ORIGIN      
        1 gcattatcat atttacgaag agatcaggat gatgacgatg aaaaaaagtg tattgacggc
       61 gtttataact gtggtatgtg caacgtccag cgttatggca gctgatgata atgctatcac
      121 ggatggcaaa gtaacattta atggtaaagt tattgctcca gcttgtaccc tggtagctgc
      181 gacgaaagat tcagtagtca cattgccaaa tgtcagcgcc accaagttgc aaactaatgg
      241 agcagtctct ggcgttaaaa ctgatgtacc aattgcctta gaaggctgtg atgttactgt
      301 aactaaaaat gcgacgttta cctttagtgg tacggctgac ggcgtgcaac ctacagcatt
      361 tgccaaccag gctaccaccg atgcagcgac gaatgttgct ctacaaatgt atctgccaga
      421 tggttctaca tcggtaactc ccggtaccga aacgtcgaat attcagttag cggattctgc
      481 tgagcaaaca gtgaccttta aagttgatta tatcgctacg gggaaagcga cttctggtaa
      541 tgtgaatgcg gtaacaaatt tccatattaa ctattattaa tagaactcat taattgtttt
      601 attaattagt acccctctag tgttctggag gggatattca tattttttaa gagtgactat
      661 ttatgaaaac ttgcataaca aagggaattg tgaccgtaag tttaacggca atattactca
      721 gctgctcatc cgcatgggcg gcaggtaaag gcgggattgg acttgcagca acacgtcttg
      781 tttattcaga aggtgaagag caaatttcac tgggtgtacg taacaccagt ccggatgttc
      841 cttatcttat tcagtcatgg gtgatgaccc cagataataa aaaatcagca gattttatta
      901 ttacaccacc gctatttgtg ctgaatccgg ctaatgagaa tctgttacgc attatgtaca
      961 ttggtgcgcc gttggcgaaa gacagagaaa cccttttctt cactagcgta cgggcagtcc
     1021 cttcaacaac gaagcgggaa gagggaaata ccctgaagat tgccacacaa agcgtcatca
     1081 aacttttctg gcgaccaaaa ggtttagcgt atcccttagg cgaggctccg gcgaaactgc
     1141 gttgcacttc gtcagctgac atggttacgg tcagtaaccc aacaccttat ttcattaccc
     1201 tgacagacct gaaaataggg ggaaaggtag ttaaaaatca aatgatttcc ccctttgata
     1261 aataccaatt ctctctgcca aagggggccc aaaatagcag cgtaacgtat cgaaccatca
     1321 atgattacgg ggcggaaacg ccacaactca actgtaaatc gtaagccgtc ttcagttaag
     1381 agagcgagat gtatagaact catcgacaac acagcctgtt aagctctggt ggagtgccat
     1441 cgtttattgg tgggctggtg gtgtttgtgt cggcagcgtt caatgcacaa gctgaaacct
     1501 ggttcgatcc tgcttttttc aaagatgatc cctcaatggt ggccgatttg tctcgtttcg
     1561 aaaaaggaca aaaaataacg ccaggggttt atcgtgtcga tattgttctg aatcagacaa
     1621 ttgtagatac gcgcaacgtc aattttgttg agataacgcc agagaagggg attgccgcct
     1681 gtttgacgac tgaaagcctg gatgcaatgg gtgttaatac tgatgcgttt ccggctttta
     1741 aacaactgga caaacaagcg tgtgtgccat tggcggagat tattccggat gccagcgtaa
     1801 cgtttaatgt gaataaactc cgtctggaaa tttcagtacc gcaaatcgcc atcaaaagta
     1861 atgcccgtgg ttatgtcccc cctgaacgtt gggatgaagg gatcaacgcg ctattactgg
     1921 gatattcgtt tagcggggct aacagtattc atagcagcgc agacagtgat tctggcgaca
     1981 gctattttct gaatttaaac agtggcgtta atttaggccc atggagattg cgcaacaatt
     2041 caacatggag tcgcagtagt ggccaaaccg cagaatggaa gaatctcagc agctatttgc
     2101 agcgggcggt tattccacta aaaggcgaac tgaccgtagg tgatgattat actgcaggcg
     2161 attttttcga tagtgtcagc tttcgtggtg tgcagctggc gtcagatgac aacatgctgc
     2221 cagacagcct gaaagggttt gcgcctgtgg tgcgtggtat cgccaaaagc aatgcccaga
     2281 taacgattaa gcaaaatggt tacaccattt accaaactta tgtatcgcct ggtgcttttg
     2341 aaattagtga tctctattcc acgtcgtcga gcggtgattt gttagttgaa atcaaagaag
     2401 cggacggcag cgtcaatagc tacagcgtac cgttttccag cgtgccatta ctccagcgtc
     2461 aggggcgaat caaatacgcg gtgacactgg cgaaatacag aaccaatagt aatgaacagc
     2521 aggagagcaa atttgcctag gccacgttgc agtggggcgg accgtgggga acgacatggt
     2581 atggtggtgg acaatatgct gaatattacc gtgccgccat gtttggtctg ggatttaacc
     2641 ttggcgattt cggagcaatt tcgttcgatg cgacccaggc gaagagtacg ctggcagacc
     2701 aaagcgaaca taaaggtcag tcatatcgtt ttctgtatgc caaaacgctc aaccaattgg
     2761 gcactaattt tcaattgatg ggctatcgct attcgacgtc gggtttctac accctttccg
     2821 acaccatgta taaacatatg gatggctacg aatttaatga cggtgatgat gaagatacgc
     2881 cgatgtggtc gcgttattac aatttgtttt acaccaaacg tggcaaactg caggtcaata
     2941 tctcccagca attaggcgag tacggttcgt tttatttaag tggtagccag caaacttact
     3001 ggcataccga tcaacaggat cggctattac agtttggcta caacacgcaa attaaagatc
     3061 tctcgctggg gggttcctgg aactacagta agtcccgtgg tcaacctgat gctgatcagg
     3121 tgtttgcact aaatttttcc ctgccgctca atctgttgct ccccagaagt aatgatagct
     3181 ataccaggaa aaaaattacg cctggatgac ctctaacacc agcatcgata acgaagggca
     3241 cattacacaa aacctgggtt taacggagac actactcgat gacggtaacc tgagctacag
     3301 cgtgcaacag ggatataaca gcgaggggaa aacggctaat ggtagcgcca gcatggacta
     3361 caaaggggcg tttgcagatg cccgagtggg ctacaactac agcgataacg gcagtcaaca
     3421 acaactgaac tacgctcttt caggcagttt agttgcccat tcacagggca ttaccctggg
     3481 gcaatcgctg ggggaaacta acgtgctgat tgcagcacca ggcgcagaga atactcgtgt
     3541 ggcgaacagc accgggctga aaactgactg gcgcggatat accgttgtcc cctatgccac
     3601 ttcttatcgg gaaaatcgaa tcgcacttga tgcggcatcg ttaaaacgta acgtggatct
     3661 tgaaaatgca gtagtcaacg tggttcccac caaaggggcg ttggttctgg cggagttcaa
     3721 tgcccatgcg ggagcaaggg tattaatgaa aacatcaaag cagggtatac cgctgcgttt
     3781 tggcgcgata gcgacgctgg acggcgtaca gactaatagc ggcattattg atgatgatgg
     3841 ctcgctctat atgtccggtt tgcccgctca gggagcgata actgtacgct ggggcgaagc
     3901 tcccgatcaa atttgtcata tcagttacca gcttaccgaa caacaaatta actctgcgat
     3961 tacgcgaatg gatgccatat gcagataatc tttggagaaa aatgcgtgtt attactacga
     4021 ctattttttg ccgccgtctt aatgctatgg tgcgctcaaa ctgctgctta tagcgggcag
     4081 tgtcatacca ctcaggggaa tccgtatatt ggcgtcaatt ttggcgttaa aaccctggag
     4141 gaagaagaaa atacggctgg agtagttaaa gacaaatttt atcagtggaa cgaatcgaat
     4201 gattattatg tttcctgtga ttgcgataaa gacaatgtca gaagtggccg atgggcattc
     4261 gccgcggatt caccgttagt ctatttaggc gacaactggt acaaaattaa tgactatctt
     4321 gccgccaaag ttttattgca ggttaaaggc agttctccta ctgcggttcc tttcgaaaac
     4381 gtgggcacag gggcagatac acgatggcat atttgcgatc ccggcggtca acgtttaggt
     4441 ggccaggggg ctagcggtaa tagcggtagc ttttccctga aaatattgca gccgttcgtt
     4501 ggttcggtcg tcattcctcc tatggcgctg gcgcgattat ttgaatgcta caacataccc
     4561 gcaggtgatt cctgcacgac tacaggtaca ccggttttag tgtattacct gtctggtacg
     4621 atcaattcac ttggctcatg ttccgtcaat gccggagaaa caatcgaggt cgatctgggc
     4681 gacgcatttg cggctaactt tcgtgttgta gggcataagc ctcttggggc cagaacggca
     4741 gaacttgcaa ttccagtcag gtgtaacacg ggaaacgcgg ggttagttaa cgtcaacctg
     4801 agtctgacgg caaccacaga ccccagctat ccccaggcga ttaagacgtc acgtcctggc
     4861 gtgggcgtgg tggtgaccga tagccagaac aacattattt cccctgctgg tggaacatta
     4921 ccgctctcta ttcctgatga tgcagacagt atcgcgcgaa tgaatgtcta tccagtcagc
     4981 acgacaggtg taccaccaga aaccgggcga tttgaagcca cggcaacggt gagaataaat
     5041 tttgattaac gcgtgaacgt atgaagaaag aaacgatatt tcagtgcgtt atcttgttct
     5101 ttagcattct taacatccat gtcgggatgg ctgggcctga acaagttagt atgcatattt
     5161 atgggaatgt ggtcgatcag ggctgtgatg tcgccaccaa aagtgcatta caaaatattc
     5221 atattggtga ttttaatatc agtgattttc aggccgcgaa taccgtaagc actgctgctg
     5281 atttgaatat cgatatcacc ggttgtgccg ctagtattac tggcgcggac gtccttttta
     5341 gcggcgaggc tgacaccctt acgccgacac tgctcaaact aactgacaca ggcggaagcg
     5401 gtggtatggc aacggggatt gccgtgcaaa ttcttgatgc gcaaagtcag caagaaatcc
     5461 cgctcaatca ggtccagcct cttacgccct taaaagccgg ggataacaca ctcaaatatc
     5521 aacttcgtta taagtccaca aaggcgggag caacgggcgg taatgcgacg gcggttctct
     5581 attttgatct ggtttaccag tgaaggagtg atgttgaaac ggataatctg gattctgttc
     5641 ttactgggat taacgggggg ctgtgagcta tttgcccatg atggcacggt caacattagc
     5701 ggatcgtttc gccgtaatac atgcgtgctg gcacaggata gcaagcaaat caatgtgcag
     5761 ttaggcgatg tctcgctgac acgttttttt catgggaatt atggcccgga gaaatctttc
     5821 atcattaatt tgcaggattg cggaacagat gtcagcacgg ttgacgtcac cttttcagga
     5881 acccccgacg gagtgcagag cgaaatgctg tccattgaaa gtggtacgga tgcagccagc
     5941 gggctggcaa ttgcgattct ggatgatgcg aagatattga ttccgcttaa ccaggcgagt
     6001 aaagactaca gcctacatag cggtaaggtg ccattaactt tttatgcgca attgcgacct
     6061 gtcaatagcg atgtgcagtc gggtaaagtg aatgccagcg cgacatttgt acttcattat
     6121 gactaatacg tggaacagat tggcgctctt gattttcgcc gttttatcgc tgctggtggc
     6181 gggtgaattg cgggcaggag tggtggtcgg cggaacgcga tttatctttc cggcagacag
     6241 agaatcgata tctattttac tgactaatac cagtcaggaa tcctggctta ttaacagtaa
     6301 aatcaaccgc ccaacgcgtt gggccggggg ggaagcgtcg acagtgccag caccattact
     6361 ggccgctccg ccacttattc tcctgaagcc cggtactaca ggcacgttgc gcttgctgag
     6421 aacggaaagc gacatcttgc ctgtggatcg cgaaacgcta tttgagttaa gcattgccag
     6481 cgtgccatcc ggcaaagttg aaaatcagag cgtaaaagtg gcgatgcgct cggtatttaa
     6541 actgttctgg cgacccgaag gtttgccggg cgacccgctg gaagcttatc aacaattacg
     6601 ctggacacgg aattcgcagg gtgtacaact cactaaccca acgccttact acattaacct
     6661 gattcaggtg agcgtaaatg gtaaagcgtt aagtaatgcg ggagttgtgc cgcctaaaag
     6721 ccagcgtcag acaagctggt gtcaggcaat cgcaccgtgt catgtcgcct ggcgggcgat
     6781 aaatgattac ggcgggttga gtgcaaagaa ggagcaaaat ctgccctgaa acaggttcgg
     6841 aaaacgtttg cgtttttttt gccgcaggtc aattcccttt tggtccgaac tcgcacataa
     6901 tacgcccccg gtttgcacac cgggaatcca ggagagttca tgtactaccc cttcgttcgt
     6961 aaagcccttt tccagctcga tccagagcgc gctcatgagt ttacttttca gcaattacgc
     7021 cgtattacag gaacgccgtt tgaagcactg gtgcggcaga aagtgcctgc gaaacctgtt
     7081 aactgcatgg gcctgacgtt taaaaatccg cttggtctgg cagccggtct tgataaagac
     7141 ggggagtgca ttgacgcgtt aggcgcgatg ggatttggat cgatcgagat cggtaccgtc
     7201 acgccacgtc cacagccagg taatgacaag ccgcgtctct ttcgtctggt agatgccgaa
     7261 ggtttgatca accgtatggg ctttaataat cttggcgttg ataacctcgt agagaacgta
     7321 aaaaaggccc attatgacgg cgtcctgggt attaacatcg gcaaaaataa agatacgcca
     7381 gtggagcagg gcaaagatga ctatctgatt tgtatggaaa aaatctatgc ctatgcggga
     7441 tatatcgcca tcaatatttc atcgccgaat accccaggat tacgcacgct gcaatatggt
     7501 gaagcgctgg atgatctctt aaccgcgatt aaaaataagc aaaatgattt gcaagcgatg
     7561 caccataaat atgtgccgat cgcagtgaag atcgcgccgg atctttctga agaagaattg
     7621 atccaggttg ccgatagttt agttcgccat aatattgatg gcgttattgc aaccaatacc
     7681 acactcgatc gttctcttgt tcagggaatg aaaaattgcg atcaaaccgg tggcttaagt
     7741 ggtcgtccgc ttcagttaaa aagcaccgaa attattcgcc gcttgtcact ggaattaaac
     7801 ggtcgcttac cgatcatcgg tgttggcggc atcgactcgg ttatcgctgc gcgtgaaaag
     7861 attgctgcgg gtgcctcact ggtgcaaatt tattctggtt ttatttttaa aggtccgccg
     7921 ctgattaaag aaatcgttac ccatatctaa ttatttcttc gaattcctct ttataagata
     7981 atcagggctt tattttcagc cctggttgtt ttatattcat cgctgttgct tatttagaca
     8041 ttttgtactt ttattgttga ggttattaag cgaagcgaca atggattgtg ttgctgcggt
     8101 tttatagggt aggggagagg cagatgcgaa ttaaaccaga cgataactgg cgttggtatt
     8161 acgatgaaga gcacgatcga atgatgctcg atttagccaa tggtatgcta tttcgctcac
     8221 gttttgcgcg caagatgttg actccagatg ccttttctcc cgcaggcttt tgcgttgacg
     8281 acgccgcgct ctatttctct tttgaagaaa agtgccgcga ttttaattta tctaaagaac
     8341 aaaaagccga actggtgctg aatgcactgg tagcaattcg ctatctcaaa ccgcaaatgc
     8401 cgaaaagctg gcattttgtt tcccatggtg aaatgtgggt gcccatgccg ggcgatgcgg
     8461 cctgcgtttg gttgagcgat actcacgagc aggtcaattt gctggtcgtt gaatctggcg
     8521 aaaatgccgc actatgcctg ctggcacaac cctgcgttgt tattgcgggt cgcgcgatgc
     8581 agttgggcga cgccattaaa atcatgaacg acaggctgaa accgcaggtt aatgttgaca
     8641 gcttcagcct cgaacaggca gtctaacgcg ccaacttaag tgcagtcttc ggtacacagc
     8701 tacagcaaag aatggtgcca tcatcgccca ttgctgattt tttcagcggc atgacttcgc
     8761 cttctaaaag ctgaacacgg caacttccac aaatgcccgc gcggcaagaa taagggatac
     8821 gaattccctg attttctaat tgctccagca acacctgttg gttatttcca cgaaatgcct
     8881 gtccctgcca gtcaatatct acatttgcgt ccggttgttg cgtgatgttg gcggtatcat
     8941 cagcggcagc tgcgccgtaa atttttgccg gagccgttgc cagaatttcc acctcatcgc
     9001 caacgcgaat cacgccgcta ttacgggcaa ttaaattctg accaaaatcg acatcgccgt
     9061 tatcctgggc agtgcggaaa gattgcaatg tttttaatgg ttcgcctgcc ggatgttttt
     9121 gccctttttc tgggctgacg gtggtgaaaa tacagcggct acaaggttta accacatcaa
     9181 acaccacatc accaatgcga atcactttcc agcgatcttc ttcccaggct gacgcgccgg
     9241 aaaccaccag attggggcgg aattgctcca tttttacact ggccggacaa cgttgttgga
     9301 gatcacgtaa cgaggcttcg ttagcaagaa ggtaaggata gccatcagca aatgacagag
     9361 gtacagtgtt gtggcgtttc acgcgccggg tcatttgtgg ccccacccag cgtaattgca
     9421 cttcgcggga gaaaaatcca cttagccatt tgttgatcgc gtctggcgca attcgcgcgg
     9481 taaaatgtgt gccccaaact tcggttggtg cgtcttgtgt ggcgaaatca gcaaaacgaa
     9541 catatgcgct actgccatct ggtgcggtga gatgcaagcc atcatgcaca ggtgaaggag
     9601 taaaccgtac catctgggga aactggcgag cggtaataaa cgtaccgtca ggttccgtga
     9661 tcataaagat gcgatcgaag gccagaccac tgacatcagc cagagcatgt gtaagaccaa
     9721 tgccgcgcat cgatttaaca ggatgaataa aaagccgggt taatgtcgcc acggtgcggt
     9781 cctcaaatga aaataagccc tcaactttat gacatgacgc gcttattagc tataatgcgc
     9841 aacaattttc ttagcgcctg tcccattagg ctattttatt tgtcagtttg gccctgggtt
     9901 gtgctcgaaa tcctcacgta cttgtgtacg ctccggtttc tccgcgctgt ccatgtccag
     9961 actgactgca acaattacgc ctactgcggt aggctcttag agtaaaagtg acgatatgaa
    10021 ttctctgttt gccagtacgg cccgtgggct ggaagagctg ttaaaaactg aactggaaaa
    10081 cctgggggcc gttgaatgcc aggtggttca gggtggggtc catttcaagg gcgacacacg
    10141 gcttgtttac cagagcctga tgtggagccg cctggcctcg cgtattatgt tgccgctggg
    10201 cgagtgtaag gtttacagcg atttagacct ctatctcggt gttcaggcga tcaactggac
    10261 agagatgttt aatcctggcg cgaccttcgc tgtccacttc agtggtttga atgacaccat
    10321 ccgcaacagt cagtacggcg cgatgaaagt gaaagacgcg atcgtcgatg ctttcacgcg
    10381 gaaaaatctg ccgcgtccaa atgttgatcg cgatgcgccg gatatccgcg ttaacgtctg
    10441 gctgcataaa gaaaccgcca gtatcgctct tgatctgagt ggtgatggtt tacatctgcg
    10501 tggctatcgc gatcgtgctg gtattgcgcc gatcaaagaa accctggcag ccgctattgt
    10561 gatgcgaacc ggctggcagc caggaacacc gctgctcgat ccgatgtgtg gttccggtac
    10621 gttgctgatt gaagcggcga tgctagcgac cgatcgcgca ccaggcttgc accgtgggcg
    10681 ttggggcttt agcggctggg tgcagcatga tgaagctatc tggcaggaag tgaaagcgga
    10741 agcgcaaact cgtgcccgta aaggcctggc tgagtatagc tctcattttt acggttcgga
    10801 cagcgacgca cgggtgattc aacgtgcgcg cactaacgcc cgtcttgcgg ggattggtga
    10861 actgatcacc tttgaggtga aagatgtcgc gcaactgacc aatccgctgc cgaaagggcc
    10921 gtacggcaca gtgttgagca acccgccata cggggaacgt ctggacagcg aaccggcgct
    10981 gattgcgctg catagcctgc tgggtcggat catgaaaaac cagtttggtg gctggaatct
    11041 ctctttgttt agtgcctcgc cggatctgct aagctgcttg cagctgcgtg cagacaaaca
    11101 gtacaaggcg aaaaacggcc cgctggactg cgtacagaaa aattaccacg ttgccgaaag
    11161 cactccagac agcaaaccgg cgatggtagc ggaagactac gccaaccgcc tgcgtaagaa
    11221 cctcaaaaaa ttcgagaagt gggctcgcca ggaaggaatt gaatgttacc gcctgtatga
    11281 cgccgatcta ccggaatata acgttgccgt tgaccgttat gccgactggg tggtggtgca
    11341 ggagtatgcg ccgccaaaaa ctattgatgc tcacaaagcg cgtcagcgtc tgttcgatat
    11401 tatcgctgca accatttcgg tactggggat tgcgccaaac aaactggtgc tgaaaacccg
    11461 tgaacgccag aagggcaaaa accagtacca gaaactgggc gagaagggcg agtttcttga
    11521 agttaccgaa tataacgctc acttgtgggt gaacctgacg gattatctcg atactggtct
    11581 gttcctcgat caccgtatcg cccgccgtat gctcggtcag atgagcaaag gcaaagattt
    11641 cctcaacctg ttctcttata ccggtagcgc caccgtgcat gcgggattag gcggcgcgcg
    11701 cagcaccacc accgtggata tgtcgcgtac ttatctggag tgggcagaac gcaacctgcg
    11761 tctgaatggc ctgaccgggc gtgcgcatcg cctgattcag gccgattgcc tggcgtggct
    11821 gcgtgaggca aatgaacagt ttgatctgat ctttatcgat ccgccaacct tctctaactc
    11881 aaaacgaatg gaagatgcgt ttgatgttca gcgcgatcat atggcgctga tgaaagattt
    11941 gaaacgtctg ctgcgtgcag gtgggacgat catgttctcg aacaacaaac gtggcttccg
    12001 tatggatctc gacggcctgg caaaactggg actgaaagca caagaaatta cgcaaaaaac
    12061 gctctcccag gatttcgccc gtaaccgcca gatccacaac tgctggctga ttaccgcagc
    12121 ctgaaaggaa tagtaatgtc attaatcagt atgcatggcg catggctgtc gttcagcgac
    12181 gcgccgcttc tcgataacgc agaactgcat atcgaagata acgaacgtgt ttgtctggtg
    12241 ggtcgcaacg gcgcgggcaa atcgacgtta atgaaaatcc tcaaccgtga acaagggctg
    12301 gatgacggtc gcattattta cgagcaagat ttgattgtag ctcgtctgca acaggagccg
    12361 ccgcgtaacg ttgagggtag cgtttatgat ttcgttgccg aaggcattga agaacaagcg
    12421 gaatatctga aacgctatca cgatatttcg cgcctggtga tgaacgaccc gagcgagaaa
    12481 aatctcaacg aactggcgaa ggttcaggaa cagttggatc accacaacct gtggcagctg
    12541 gaaaaccgca tcaacgaagt gctggcgcaa ctggggttag atcctaatgt tgcgctgtcg
    12601 tcgctttccg gcggctggtt gcgtaaagcg gcattaggac gcgcgctggt gagtaatccg
    12661 cgcgtgctgt tgcttgatga accgacaaac cacctggata ttgaaaccat cgactggctg
    12721 gaagggttcc ttaaaacctt caatgggacg attattttca tctcccacga ccgttcgttt
    12781 atccgcaata tggcgacgcg cattgttgat ctcgatcgcg gcaagctggt gacctatcca
    12841 gggaattacg accagtacct gcttgaaaaa gaagaagccc tgcgcgtgga agaactacaa
    12901 aatgccgagt tcgatcgcaa actggcgcag gaagaggtgt ggatccgcca ggggatcaaa
    12961 gcacgccgta cccgtaatga aggccgcgta cgcgccctga aagcaatgcg tcgcgagcgt
    13021 ggtgaacgtc gcgaagtgat gggtaccgca aagatgcagg tggaagaggc cagccgctcc
    13081 ggtaagatcg ttttcgaaat ggaagacgtt tgctaccagg ttgacggtaa gcaactggtg
    13141 aaagattttt cggcccaggt tctacgtggc gacaaaattg ccctgattgg tccgaatggg
    13201 tgcggcaaaa ccacgctgct gaaactgatg ctcgatcagc ttcaagcgga cagcgggcgt
    13261 attcacgttg gcaccaaact ggaagtggct tatttcgatc agcaccgcgc ggaactggat
    13321 cccgataaaa cggtgatgga taaccttgcc gaaggtaagc aagaggtgat ggttaacggc
    13381 aagccacgcc acgtattggg ctatttgcag gactttctgt tccatccgaa acgggcgatg
    13441 acgccggtac gtgcgctttc tggcggtgag cggaaccgct tgctgctggc gcgtttgttc
    13501 ctcaaaccaa gcaacttatt gattcttgac gaaccgacca acgatcttga tgtcgaaacg
    13561 ctggaactgc tggaagaact gatcgacagc tatcagggca cggtattgct ggttagccac
    13621 gatcgtcagt ttgtcgataa caccgttaca gaatgttgga tcttcgaagg cggcggtaaa
    13681 attggtcgtt atgtcggcgg ttatcatgat gcccgtggtc agcaagagca gtatgtggcg
    13741 ctcaaacagc ctgcggtgaa aaaaaccgaa gaagccgccg cggcaaaagc agaaactgta
    13801 aaacgcagca gtagcaaact aagctataaa ttgcagcgcg aactggagca gctaccgcaa
    13861 ttgctcgaag atctggaggc gaagctggaa gccctacaga cgcaggtggc ggatgcttcc
    13921 ttcttcagtc agccacatga gcagacacaa aaagtgctcg ccgatatggc tgctgcagag
    13981 caggagctgg agcaagcctt tgaacgctgg gagtatcttg aagcgttaaa aaatggtggc
    14041 tgatcgcaaa atagtcgatt taaacagaga cagctgacaa cggtaaaatt gcctgatgaa
    14101 ctgggtttat caggcatgaa aaccaacact ttgttagcca tctgtaaaat agcgcatcat
    14161 taaggagtac caatgtgcga acatcatcat gccgcgaagc acatcctgtg ctcgcagtgt
    14221 gacatgctgg tggcgttacc gcgccttgag catggtcaga aagcggcatg tccccggtgt
    14281 ggcacaacgt taaccgtggc gtgggatgcc cctcggcagc gtccgaccgc ctatgcgttg
    14341 gctgcactgt tcatgctgtt gctgtccaac ttgtttcctt ttgtgaatat gaacgttgca
    14401 ggagttacca gtgaaattac attactggaa attcccggcg tgcttttttc tgaggactac
    14461 gccagcctcg gcaccttttt cctgttgttt gtgcaactgg ttcccgcgtt ttgtctgata
    14521 accattctgt tactggtgaa tcgcgcggaa ttaccggtcc gtttaaaaga gcaactggca
    14581 cgggtgcttt ttcaactcaa aacctgggga atggcggaga ttttcctcgc tggtgtgctg
    14641 gtcagtttcg ttaaactgat ggcttacggc agcattgggg taggcagcag ctttctcccc
    14701 tggtgtttat tttgtgtcct gcaactgcgc gcttttcagt gcgttgatcg tcgctggtta
    14761 tgggacgaca tcgccccgat gccagaactg cgccagccgc taaaaccagg cgtcacgggg
    14821 atacgtcagg ggctgcgttc gtgctcctgt tgtacggcaa tccttcctgc tgatgaaccc
    14881 gtgtgcccgc gttgtagtac caaagggtac gttcggcgta gaaacagcca gcagtggaca
    14941 ctcgcgctgc ttgtaacgtc catcatgctg tatcttccgg ctaatatttt gcccatcatg
    15001 gtgacggatt tattaggctc gaagatgccg tcgacgattc tcgctggggt cattctgtta
    15061 tggagcgaag gctcttatcc cgtcgctgcg gtgatctttc tggccagtat tatggtgcca
    15121 acgttaaaga tgatcgccat cgcgtggctg tgttgggatg ccaaagggca tggcaagcgc
    15181 gacagtgaaa gaatgcattt gatttatgaa gttgttgagt ttgtaggccg ctggtcgatg
    15241 attgacgttt tcgttatcgc ggtgctctcg gcgctggtgc gtatgggagg tttaatgagt
    15301 atttatccgg caatgggtgc attaatgttt gctttagtcg tcataatgac aatgttttct
    15361 gctatgacgt ttgacccgcg tttgtcgtgg gatcgtcaac ctgaatcaga gcatgaggag
    15421 tcctgacagt atggaatcta ataatgggga agccaaaatc cagaaagtga agaactggtc
    15481 tcccgtgtgg atatttccta tcgtcacggc gctcattggg gcctgggttc ttttttatca
    15541 ttacagccat cagggactgg aagtgaccct gatcaccgcg aatgcggaag gaattgaagg
    15601 tggcaaaacc accattaaaa gccgtagcgt tgacgtcggc gtggttgaaa gcgccacact
    15661 ggctgatgat ttgacgcacg ttgaaatcaa agtgcggctg aattctggta tggaaaaatt
    15721 gctgcataaa gacaccgtct tttgggtggt gaaaccgcag attggtcgcg aagggattag
    15781 cggcctggga acgctgctgt ctggagttta tatcgaactg cagccaggcg cgaaaggcag
    15841 caaaatggat aaatacgatt tgctggactc gccaccgttg gccccgcctg atgcgaaagg
    15901 tatccgtgtg attctcgata gcaaaaaagc cgggcagctc tcgccaggag atccggtgct
    15961 gttccgtggc tatcgggtag gttcggttga aaccagcacc ttcgatacgc aaaaacgcaa
    16021 tatcagttat caactgttca tcaatgcacc ttatgaccga ctggtgacca gcaatgttcg
    16081 cttctggaaa gatagtggca ttgcggttga tctgacgtca gcggggatgc gtgtggagat
    16141 gggctcattg acaacgctgc ttagtggcgg tgttagcttt gatgtgccgg aaggtctgga
    16201 tttagggcag ccagtggcac cgaaaacagc tttcgttttg tatgatgatc agaagagcat
    16261 tcaggattcg ttgtacaccg atcacattga ttatctgatg ttctttaaag attcggtacg
    16321 cggtctgcaa ccgggagctc cggtagaatt ccggggtatt cgcctgggta ccgtaagcaa
    16381 agtgccattc tttgcgccga atatgcgtca gacatttaac gatgattacc gtattccggt
    16441 actgattcgt atcgagccag agcggctgaa aatgcagctt ggcgaaaatg cggatgttgt
    16501 tgagcacctt ggcgaattgt tgaaacgtgg tttacgcgga tcgctgaaaa ccggaaacct
    16561 ggtcactggt gcactgtatg ttgatcttga tttctatcca aatacgcctg caataaccgg
    16621 tattcgtgaa tttaatggtt atcagattat cccgactgtt agcggcggcc tggcgcaaat
    16681 ccagcaacga ctgatggaag cgttggataa gatcaacaaa ctgccattga atccgatgat
    16741 tgaacaggca accagtacgc tttctgaaag tcagcgcaca atgaaaaacc tgcaaacgac
    16801 gctggatagc atgaacaaga tcctcgccag ccagtcgatg cagcagttgc cgacggatat
    16861 gcagtcaacg ttgcgtgaat tgaatcgcag catgcagggc ttccagcctg gctccgcagc
    16921 ctacaacaag atggtggcgg atatgcagcg ccttgatcag gtgttgcgag aactgcaacc
    16981 ggtgctgaaa acgctcaatg agaagagtaa cgcgctggta tttgaagcga aggacaaaaa
    17041 agatccagag ccgaagaggg cgaaacaatg aaaaagtggc tagtgacgat tgcagcactg
    17101 tggctggccg gatgcagctc cggcgaaatt aataaaaact attaccagtt acctgtggtg
    17161 cagagcggta cacaaagtac cgccagccag ggcaatcgtc tgttatgggt agagcaggtc
    17221 actgttcctg actatctggc ggggaatggt gtggtttatc aaaccagtga tgtgaagtat
    17281 gtgattgcca acaacaactt gtgggccagc ccgttggatc aacaattgcg caacaccctg
    17341 gttgccaacc tgagtacgca actgcccggc tgggtggttg cctcccagcc tctgggaagc
    17401 gcccaggaca cgctcaatgt taccgtaacg gagtttaacg gtcgctatga tggcaaggtc
    17461 attgtcagtg gtgagtggct gttgaaccac cagggacaac tgatcaaacg tccgttccgt
    17521 ctggaaggag tgcaaactca ggatggttac gatgagatgg ttaaagtact ggccagtgtc
    17581 tggagtcagg aagccgcttc tattgcgcaa gagataaagc gtctacctta attataaaga
    17641 tttgtaaata taaccgtctc cggtatgttg cctgaggcgg tttttttgtt tctaacgtgc
    17701 ggaaaaagtt gttcctcttt acattttttg tacaaccgac atacccgtgt agctcacaaa
    17761 tatgacagtg gcgtgaattt tgcgcattga cggcagttat gattcgcggt attgcttaac
    17821 tgtgattgca catttagtaa tcactgtttt cttttccacc agaaaccagt atgagggaaa
    17881 cgaggcatga agagacaaaa acgagatcgc ctggaacggg cacatcaacg tggttatcag
    17941 gccggcatcg ccggacgctc aaaagaaatg tgtccctatc agacgctgaa tcaaaggtca
    18001 caatggctgg gaggctggcg agaagccatg gcggacaggg tagtaatggc ctgattctgt
    18061 ctctttaaaa agaaacctcc gcattgcgga ggtttcgcct tttgatactc tgtctgatta
    18121 taatcagaag gcagaagtat cctggaacag accgactttc aggtcgctgg cggtatagat
    18181 caaacgacca tcaaccagca cttctccatc cgccaggccc ataatcagac gacggttaac
    18241 aatgcgttta aagtgaatac ggtaggtcac ttttttcgct gtcggcagta cctgaccagt
    18301 gaatttcact tcgccaacgc ccagtgcgcg gcctttacct tcgccgccca gccagccgag
    18361 gtagaaccct accagctgcc acattgcgtc caggcccagg catcccggca taaccggatc
    18421 gccaataaag tggcatccga agaaccacag atccggattg atatccagtt ctgcttcaac
    18481 ataccctttg tcgaagttac cacccgtttc ggtcattttg accacacggt ccatcatcag
    18541 catattcggt gctggcaatt gcgggccttt agcgccaaac agttcaccgc gaccagaggc
    18601 aagaaggtct tcttttgtat aggattcgcg tttatctacc atgttctctg taagccttat
    18661 tttattgaag cacgcaggat agctaacacg tgtacgctga acaagtccga tcagttcgga
    18721 ataaaccagt tcagccaacg taatggccat ggaaaacggt gacgtccttc ctgttgcgat
    18781 gcttgcgcga tacgttcctg gatggtttgc atcagcgtcg tttggccttc gccatcccac
    18841 accagattta ataataacgg cagtgcgtca gtcacatcgt ctactgccca gatggtgaat
    18901 ttgccttctt ctaccgcttt cactagttca ctgtgaagac ttaaatggcg aacgttagct
    18961 gtggggataa tgacaccttg tttcccggtt aactcacgtt gctggcaaat agcaaagaag
    19021 ccttcgattt tctcatttaa accaccgacc ggctgggcgc gaccgaactg atcgactgaa
    19081 cctgtgatag cgatactctg attcaccggc acatcggcga gggcgcttat cagggcgcag
    19141 agttcagcca tcgaggcact atctccatca acttcactgt atgactgctc aaatgtcagc
    19201 gatgctgaga aggggatctg ttgctcaagc tgtagttccg acatcaggaa cgcttgcatg
    19261 atcatcatcc ctttcgcatg gatattgccg ccaagctccg ctttgcgttc gatgtcggtg
    19321 aattcaccat cgccaatatg cacaacgcag ctaatgcgag aaggttcgcc aaaagcgcgt
    19381 ggatgacccg gaaattcaat gaccgaaagg gcgttaattt gcccgatgcg ttcgccttcg
    19441 gtttcaatca ggatttgctc ctgaaggatc tcatcctgca tacgttcagc gaggaaacct
    19501 tcgcgccatt cacgctgctg cagcattaag tttagctgct cgccggagaa ggtgtcgcca
    19561 tcacacaggg aggcgacctc tttacactgg cggaggatcc actgcgggct aagcggaagt
    19621 gtttcttgtt caccggtgta gcgtgctgct tcgcggataa gtaccggcca ggcatccgct
    19681 cccggtgcag gtaagtgatt atgtctggcg gtaaatgtca cccagcgaca ccactgggtt
    19741 actgactccg catcgacaat ctgcagagta tcttcaaatt cgctataaat agcctgctct
    19801 gaaagctctg gctccatctc ctggaaatca gccaatgatt cgcgttcgcc taccagaatg
    19861 actttcagct tcaatggcat cgaaggcaca gagacgggga gagggcgcga ctcatcaaac
    19921 gcaacccagt caaaacgctc gcggttaacg atatttttca gccgcatcca cagcagaggt
    19981 tgcgccagca gtgtacgcaa agagatgatg agaataccgc catttgcctg atgcaccaga
    20041 ccaggctgca gggtaatgtc gccattaaac tggcgcaggc agccaaagag ttgctccgct
    20101 tctacccagt cggcagcgac aacttgcgtt aaagtcgcaa aattatcatc tgcactcact
    20161 gcgtgacgta agcggatgga gtggccggaa acctcatagt gaccgcccac cagttggcct
    20221 gcatcgcttt gtagcgtacg cgcggcattg gcaataagat tcagatactc agactcttcc
    20281 ggggccttcg ccagcataaa agaggaggat gctcgcgtat gcagcaactg ctccagcgca
    20341 aattgcagcc gcggttgagt atcactgaat aaaggatcgt tttcgtcaat caaatgtggc
    20401 tgagcaaata tttcctgata gctatcggta tcaggaacca ggtcacgcca tgcaagtttc
    20461 gtaatggtca aagttgatgt tttttagtct gttgtcaaag ccgcgattat accgtaaccg
    20521 gcactacagc acacgtagaa agcaccgaca atactccagg catgggcgtt aaagctcaca
    20581 ggatggagat tcttttcttc actggcctaa aaagctgata ttctgtaaag agttacacgg
    20641 taacattgag atcgctatga aatatcaaca acttgaaaat cttgaaagcg gttggaaatg
    20701 gaagtatctg gtgaagaagc atcgcgaagg ggagttaatc acccgttaca tagaagccag
    20761 tgccgcccag gaagccgtcg atgtgttgct ctcgctggaa aatgaacccg tgctggtaaa
    20821 tggctggatt gacaagcata tgaatccgga actggtcaat cgcatgaagc aaaccattcg
    20881 ggcaagacga aagcgccatt ttaatgcgga acaccagcat acgcgcaaaa aatcgatcga
    20941 tctggaattt attgtctggc aacgtctggc tggtcttgcg cagcgtcgcg gtaaaacgct
    21001 ttctgaaacg attgttcagc tgattgaaga tgcggaaaac aaagagaaat acgcgaataa
    21061 aatgtcttct ctgaagcagg atctgcaggc attgctgggt aaggaataac tgacgaaagt
    21121 cagttcaatt tactaaaggc aaaaaaaacc ccgcagcagc ggggtttttc taccagacga
    21181 gaacttaagc ctgcggctga gttacaacgt ctttgatacc tttaacttcg atctctacgc
    21241 gacgatccgg agccaggcag tcgatcagtg cagcacgctg tttcacgttg tcacaggtgt
    21301 tgccagtaac cgggttggat tcgcccatac cacgtgcgga gatcttgtct gccgggatac
    21361 ctttggagat caggtaatca acaacagact gagcacggcg ctcggacaga ccctggttgt
    21421 aagcgtcaga accgatgcgg tcggtgtaac ccagaacaac tacggaaccg tctttcggat
    21481 ccaggttgct cagctggctg tacagctgat ccagagcagc ctgaccttcc ggtttcaggg
    21541 ttgctttgtt gaagttgaac agaacgtcag acttcagagt gaagtgcttg gtctgtactt
    21601 ccggtgccgg agctggagcc ggagcaacta ctggagctgc ttcgccctga ccgaaacggt
    21661 aggaaacccc caggctcagc atgccgttgt ccggacgagt gccgatggtg tgtgcgtcac
    21721 cgatgttgtt ggtccactgg tattccagac gggtagcgat ttcaggagtg atcgcgtact
    21781 caacaccgcc agcgaagacc ggagaaacgc cggtgtcgtg gtttttacca taaacgttgg
    21841 atttagtgtc tgcacgccat accataccac ccagacgagt gtagatgtcc aggtcgtcag
    21901 tgattgggta acccagttta gcggtcagtt gaacgccctg agctttgtat gcaccgtttt
    21961 caacgctgcc tttgtacggc atacgaccta accagtcgta acccatttca aagccaacat
    22021 acgggttaac ctggtaacca ccaaaagcac cagcgcccag ttggttttca tgggtcgggc
    22081 cattgttgtt gatgaaacca gtgtcatggt actgggacca gcccagttta gcaccagtgt
    22141 accaggtgtt atctttcgga gcggcctgcg ctacggtagc gaaaccagcc agtgccactg
    22201 caatcgcgat agctgtcttt ttcatttttt gcgcctcgtt atcatccaaa atacgccatg
    22261 aatatctcca acgagataac acggttaaat ccttcaccgg gggatctgct caattaactc
    22321 taccgatatc ttcggcttat gccgagcacc cctggcgatg taaagtctac aacgtagttg
    22381 aaaacttaca agtgtgaact ccgtcaggca tatgaaaaaa aagtcttgta taaggtatgt
    22441 ttaatctttt ttgtcagcga caatttacag aagaaaatcg cggaaaccgc ttcagacaag
    22501 cctccgcaag gaaaattagt cacgactgaa agcattggct gggcgacaaa aaaagttcca
    22561 ggattaatcc taaatttact taatgataca aattagagtg aatttttagc ccggaaagtt
    22621 gtctcgtggc gtgagaggat gcgcttaccg gacgcataat aaaccccata gcgttacctt
    22681 catttgccgc atcaacaagt tcagcatgct cttcttcagt caaatcatct gccaaccaac
    22741 cgatcaccac actgtaattg cccgtgcgta aagcgcgaac cattgactcc acagtgtggc
    22801 aaggggagag ctggctaatc tgcattactt tcgttaaggg tagcccagat gcctgaaccc
    22861 attcccgact cagtttttgt tgcggtgtta accagagttg ccagcgcgat tgctgaccga
    22921 gttgctgtaa caatggcaac agtagaagtt gcgtcatcat gggctgatct tcgcgataga
    22981 caacttcact gataagcccg gctgtagtgt tttccgtaga gacacgcgca attttacttg
    23041 ctgcggatga gaacgacgaa gaacgatgtg catagcctga agtgtacata atcaatccag
    23101 cccctatgag ttactgtatg gatgtacagt acatccagtg acaacaaaga tcaaccctat
    23161 tttcggaaag agcctcgcaa attttgtcgt tggtgacggg aaaacataaa ttaatcttgc
    23221 cccttaagaa taagttgcct attttcgtag ttaacggatc cgttaatgtg aatcattctt
    23281 ttatgttatg attttaaaag gaattttatg aaaagcctct cctataagcg gatctataaa
    23341 tcacaagaat acctggcaac gttgggcaca attgaatacc gatcattgtt tggcagttac
    23401 agcctgaccg ttgacgacac ggtgtttgcg atggtttctg atggtgagtt gtatcttcgg
    23461 gcttgtgagc aaagtgcaca gtactgtgta aaacatccgc ctgtctggct gacatataaa
    23521 aagtgtggcc gatccgttac cctcaattac tatcgggttg atgaaagtct atggcgaaat
    23581 caactgaagc tggtgcgtct gtcgaaatat tctctcgatg cagcgctgaa agagaaaagc
    23641 acgcgcaata cccgggaaag actgaaagat ttgcccaata tgtcttttca tctggaagcg
    23701 attctcgggg aggtggggat taaggatgta cgggcgttac gtatacttgg ggcaaaaatg
    23761 tgttggttgc gactgcggca gcaaaacagt ctggtgacag aaaagattct gtttatgctt
    23821 gaaggtgcca ttatcggcat tcatgaagct gcgctcccgg tggcacgccg ccaggagctt
    23881 gcagaatggg ctgactctct tacgccgaaa caggagtttc ctgcggaact tgagtaatct
    23941 ggcgttgcag acgaccaatc tcaggcagta atgcaatcaa taatccaact tgttgtacga
    24001 ccagaggttc tttgctgtct gcccgtggtt caagttgctg catccgctgt ttcaggctag
    24061 ctaatgcctc attgacgcgt tcttcatcag caggttgatg atgtaacgcg tcatcaacat
    24121 agcaaactgc gtcatcaaga aacgccagaa tttcaggatt agttaactgc tcccggtgag
    24181 caccgagggc tgagatatag ctggtaaacg tatggttaag gcacagcaac cgaaacgcgg
    24241 cttcgcgaat ttgcggggta acgttcggct cgctggacat atttgatacc accgacgcca
    24301 gctcagcatc acggttgtgt gcatcgcggc gggcaatacg atacgccaga cggttatcac
    24361 gcccctgatg gtattgctcc agtatggcat cgagataccg acagttggcc tctgtggcgc
    24421 gttcgagcat gcgcggcaga ttgcgaaact gccagtcagg ccagatgtag ctcactgccg
    24481 cccacgcaat ggcacaacca atcagcgtat cgattacgcg aggtaacgct acttcaaaac
    24541 cttcacccag taagttaaaa cacagtagca ccaaaagtgt gatgaacatc gttgcatgag
    24601 cgtattgcac gttacggaag gcaaaaaaga gcacgccggt aataaccagc agcaccagct
    24661 gcccttccag tgatggcaca aaccacagca caggaatgcc aatggcgata cctaccagcg
    24721 taccaataat ccttaacttc aggcggtggc gcgtggcgtt atagtttggc tggcagacaa
    24781 acaaacttgt cagcaagatc caatacccgt gatgcattcc ggttatctga atgatggcgt
    24841 agccgaagca caataccagc gacattctta ccgcatgacg gaagagggcg gattccggcg
    24901 tgaagtgacg gctaagacgc agccagatat cactcaaccc gtgcgggctg tcatcagcga
    24961 gctcattttc gtcattatta tggggtagtg cctgggcctg ttctgattca attgttgcca
    25021 gttgggcatc aatggcgcgt aaattgttca gcaaaaatcc cagtgttttg agtaaatcgg
    25081 cgggtgcgcc gttatcgcgc atccgctcca gcgcagcatc aatatgcgta aaagcgcgct
    25141 caaaatgcgg atcatgttga taaggctgac gcaacaaaat acagcgtgac agttgctggc
    25201 acgcctggcc ctgcatcgac atcagccgct gaaaacggaa cagcacgtcg ctgtggcgaa
    25261 aatgttcacg caatgtttga tactgaatat gagaagagct ggcacgctcg tgaatatcct
    25321 gtgcgacaaa gtaataatgc agcgtgcgac gcgttccccg ttgaccacga tcgccacgta
    25381 agcgggtcag cagcgagagt ttcgtctgat tcaatgtcgc catcagcaga ccgttggcga
    25441 gagccaaatc gtacagcggt gcctggcttt catcttcaat atcaggatca aacatgcgcg
    25501 acttgagctc aagataacgc gccagttgtt catagcaacg cgccaggttg tcctgcagcg
    25561 ggcggaccgg gaacagcaga tgaccaataa gtgtcaggac gttgtaccag acggcaccgg
    25621 ccagcagata catcggctgc tgataccagt gctcatacag tgatgttccc aacatagtgt
    25681 aaatggcgat cagcaatgca ccgaaggcaa ttgttgcata gcgttgaccc agaccgccga
    25741 gcaaaatgaa gccgctggta gagagcgtta agccaatcgc aaatagccag ggccagggaa
    25801 acagcaattc tactgaggcc gaggcgataa aaaagcagaa cagcgtaatg atgaggttac
    25861 gcaaacgtcc cgccagtcgg tcatcgagat cggtcagcgc cgctgccacc atccccagcg
    25921 ttagcggaat cgtcagtttt acatcaccca gccaccacgg aaacgctgtg gttccacaaa
    25981 gcgcaataaa aatacgcgcg taatacagcc aggcgctgtt ccaggtatag cgtttgagca
    26041 aaggacttag cataaaggcc atattagtgg attattcaaa acgacgacgt gcattggctt
    26101 cacgcgcagc ttgcgctgtt tctaccgata ccacgcgacg accaaccggc catagtgcaa
    26161 tagcggcaat tttaaagttc gcaatgccga caggaatgcc aatgattgaa atacattgtg
    26221 cgatgcccgt tgcaatgtgc atcaggcata accaccagcc aaagaaaatc agccagaaaa
    26281 tattcaatac cgtaccgcca gtattcagca gcacattttt gccagccggg ttcagttcat
    26341 cgacatgaat agcttcattg ccataaggca ccagagatag tttagtgatc tcccagcagg
    26401 atcgtgtcag cggtaaggta aaaatcagca caatactgac cagagtcgcc aacagccagc
    26461 ccagagtggt ggcaaatccg ccaagcacaa agttcagaat gttcaaaacg gtacgcataa
    26521 aacctcgctt tactgtggtt ttcagtaatg ggcggcaatt gtaacgtttt tttgggctgg
    26581 agcacgtttt ctctgacggt tacactgata agaaataatt tcgtgtggat ctacagagtc
    26641 atggaactga aagcgacaac gcttggaaaa cgtctggcac agcaccctta cgatcgggcg
    26701 gtgatcctca atgccgggat taaagtctcc ggcgatcgcc acgaatacct tattcctttc
    26761 aatcaattac tggcgattca ctgtaagcgc ggtctggtat ggggcgagct ggaatttgta
    26821 ctgccggacg aaaaagtggt gcgtctgcac ggcaccgaat ggggcgagac gcagcgtttt
    26881 taccatcatc ttgatgctca ctggcggcgg tggagtggcg agatgagcga aattgcgtct
    26941 ggtgttttac gccagcaact ggatttgatt gccacgcgca ctggagaaaa taaatggctg
    27001 acgcgtgagc aaacctctgg cgttcagcaa caaatccgcc aggctttgtc ggcgttgccg
    27061 ttgccggtta accgactgga agaattcgat aactgccgtg aggcgtggcg taaatgtcag
    27121 gcctggttga aagatattga aagcgctcgg ttgcagcata accaggcgta taccgaagcc
    27181 atgcttaccg agtatgcgga ttttttccgc caggtcgagt cttcaccgct gaatccggcg
    27241 caggcccggg cagtcgttaa tggcgagcat tctctgttag tgctggcagg tgcaggaagc
    27301 ggaaaaacgt cggtgctggt ggcccgtgca ggctggttgc tggcgcgtgg tgaagcgtcc
    27361 cctgagcaaa ttttattgct ggcgtttggt cgcaaagccg ctgaagagat ggacgagcgg
    27421 attcgcgaac ggctacatac cgaagacatt accgcacgca cgtttcatgc gctggcgctg
    27481 catattattc agcagggcag caaaaaagtt ccgatagtca gcaaactgga aaatgatacc
    27541 gctgcccgtc atgaactctt tattgctgag tggcgcaagc aatgcagcga aaagaaagcg
    27601 caggcgaagg gctggcggca atggctgacg gaagaaatgc agtggtcagt gccagaaggt
    27661 aacttctggg atgatgaaaa attacagcgt cgccttgcct ctcgcctcga tcgctgggta
    27721 agtctgatgc ggatgcacgg tggtgcacag gcagaaatga ttgccagtgc acccgaagag
    27781 attcgcgatc tgttcagtaa acgtatcaag ttgatggccc cgttattaaa agcctggaaa
    27841 ggtgcgctga aggcagaaaa cgctgtcgat ttttcgggcc ttattcatca ggcgattgtg
    27901 attctggaga aaggtcgctt tatcagcccg tggaagcata ttctggttga tgaatttcag
    27961 gatatctcgc cgcagcgggc agcgttgtta gcggcattac gcaagcaaaa cagtcagacg
    28021 acgttgttcg ctgttggtga tgactggcag gcgatttacc gattcagcgg tgcgcaaatg
    28081 tcgctcacca ccgctttcca tgaaaacttt ggtgaaggcg aacgttgtga tttagacacg
    28141 acttaccgtt ttaacagtcg tatcggtaag gtggcaaacc ggtttattca gcagaaccca
    28201 ggccagctga aaaagccgct aaacagctta accaatggag acaaaaaagc cgtcacgtta
    28261 ttggatgaga gtcaacttga cgctttgctg gataagctct ctggttatgc caaaccggaa
    28321 gagcgcattc tgatcctggc gcgttaccat cacatgaggc ctgccagcct ggaaaaagcg
    28381 gcaacacgct ggccgaagtt gcaaatcgac tttatgacca ttcatgccag caaagggcaa
    28441 caggcggatt acgtcatcat cgttggcttg caggagggaa gtgatggttt tccggctgcg
    28501 gcgcgggagt cgattatgga agaggcgcta ctgccaccgg ttgaggattt cccggacgct
    28561 gaagaacggc ggttaatgta cgtggcgctg acccgggcac gccatcgggt atgggcactg
    28621 tttaacaaag agaatccctc tccctttgtg gaaatactga aaaatctgga tgtgccggtg
    28681 gcgagaaaac cgtaagaaac aggtggcgtt tgccacctgt gcaatattac ttcagacggt
    28741 ccgcgagata acgctgataa tcggggatta gaatatcgac cgcgtcgttg aaatgcggcg
    28801 actggattat gaagtctgcc gttgccacgt tggtggcgac cggaatgttc cataccgtcg
    28861 ccagacgcag caaggctttc acgtcaggat cgtgcggcac ggcatttagt ggatcccaga
    28921 agaaaatcaa tacatcaatt ttcccttctg agatcaatgc gccaacctgc tggtcacccc
    28981 ccattgggcc actcaacatc gcgttgacgt tcatgccggt cgcgcgggaa attaagttac
    29041 cggtagtgcc tgttgcatac agtacgtgtt gttccagtaa cggttgatgc cgttccaccc
    29101 agctcatcag catttgtttg cagtgatcgt gtgccaccag cgcaatatgt ttccgcgcag
    29161 gtaaagtgcg agtcgtcagt tccataatgt acatccgtag ttaactttcc tacagattac
    29221 tgtaagcact tatcgctgca agataaagac cgaaaaagcc tgcgcacagg cacaaaaatc
    29281 tcaggaagat ggttgttttt ccgcccactg caggaaagta tttcgcgttt gtgggtcagc
    29341 cagtttaaac caatacttca gccgttgttc tgtgagcacc tgagactgcg gtggaatagc
    29401 atccagctcg gaaacacccg aaagcagcgt actgtcatct gccatcatcg tggcaaattg
    29461 cggtagtgaa gcgcgtttcg cggatttgtt atagcgttca acctcgacct cataatcaat
    29521 cgtttttgcc gttgaggtaa tggcgagaat atccagcttt accggaatcg gcgtcgcatc
    29581 gccatccaac aattcaaggc gcggcgcggc atcaaaatgg ttagcttccc gctcattctc
    29641 caggcgaggc aggcgaaaat tgacctggtt gatgagctgg gtattaaaac tgaccaccag
    29701 cggtggggag atatacagcc gttcttcact attggaaaga tgaatcgtct tctcaacgcg
    29761 aaacactaac tgatgcggtc cattatccag ttcaatgctg tccgcaccgc gcagcagaga
    29821 gctggaaacc ttcttgccgt cgagtaccag aagatcgaca tcggttgaaa gccgcagcgt
    29881 ggtggcaaaa acggataccg gcaaacataa tgcaattaag gtggtcacga tgccggtttt
    29941 cataggtagc tcctgtcaaa agaccgcccg aataataatg tgtcaaaatt tttctttaat
    30001 c
//
LOCUS       DAEEKQ010000031        29548 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.031, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000031 DAEEKQ010000000
VERSION     DAEEKQ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29548
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.031"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..689
                     /gene="dgt"
                     /locus_tag="KLK57_001407"
     CDS             <1..689
                     /gene="dgt"
                     /locus_tag="KLK57_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dNTP triphosphohydrolase"
                     /protein_id="HBE0891816.1"
                     /translation="EDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRS
                     NSLSRSTEDQFFMYLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLL
                     KLYKNVAVKHVFSHPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRF
                     PIESRLFHKLSTRHRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWD
                     EYRRLMAVEQ"
     gene            819..2243
                     /gene="degP"
                     /locus_tag="KLK57_001408"
     CDS             819..2243
                     /gene="degP"
                     /locus_tag="KLK57_001408"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HBE0891817.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            2398..3555
                     /gene="cdaR"
                     /locus_tag="KLK57_001409"
     CDS             2398..3555
                     /gene="cdaR"
                     /locus_tag="KLK57_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HBE0891818.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(3644..4030)
                     /locus_tag="KLK57_001410"
     CDS             complement(3644..4030)
                     /locus_tag="KLK57_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HBE0891819.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="KLK57_001411"
     CDS             complement(4192..5004)
                     /gene="yaeI"
                     /locus_tag="KLK57_001411"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase YaeI"
                     /protein_id="HBE0891820.1"
                     /translation="MISRRRLLQATAATIATSSGFGYMHYWELGWFELIRHRLAFFKD
                     NAAPFKILFLADLHYSRFVPLSLISDAIALGIEQKPDLILLGGDYVLFDMPLNFSAFS
                     DVLSPLAECAPTFACFGNHDRPVGTEKNHLIGETLKSVGITVLFNQATVIATPNRQFE
                     LVGTGDLWAGQCKPPPASEANLPRLMLAHNPDSKEVMRDEPWDLMLCGHTHGGQLRVP
                     LVGEPFAPVEDKRYVAGLNAFGERHIYTTRGVGSLYGLRLNCRPEVTMLELV"
     gene            complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="KLK57_001412"
     CDS             complement(5059..5883)
                     /gene="dapD"
                     /locus_tag="KLK57_001412"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HBE0891821.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="KLK57_001413"
     CDS             complement(5914..8586)
                     /gene="glnD"
                     /locus_tag="KLK57_001413"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HBE0891822.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLHYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(8648..9442)
                     /gene="map"
                     /locus_tag="KLK57_001414"
     CDS             complement(8648..9442)
                     /gene="map"
                     /locus_tag="KLK57_001414"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HBE0891823.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFIEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            9810..10535
                     /gene="rpsB"
                     /locus_tag="KLK57_001415"
     CDS             9810..10535
                     /gene="rpsB"
                     /locus_tag="KLK57_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HBE0891824.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            10793..11644
                     /gene="tsf"
                     /locus_tag="KLK57_001416"
     CDS             10793..11644
                     /gene="tsf"
                     /locus_tag="KLK57_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HBE0891825.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            11791..12516
                     /gene="pyrH"
                     /locus_tag="KLK57_001417"
     CDS             11791..12516
                     /gene="pyrH"
                     /locus_tag="KLK57_001417"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HBE0891826.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            12808..13365
                     /gene="frr"
                     /locus_tag="KLK57_001418"
     CDS             12808..13365
                     /gene="frr"
                     /locus_tag="KLK57_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HBE0891827.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            13457..14653
                     /gene="ispC"
                     /locus_tag="KLK57_001419"
     CDS             13457..14653
                     /gene="ispC"
                     /locus_tag="KLK57_001419"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HBE0891828.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            14842..15600
                     /gene="ispU"
                     /locus_tag="KLK57_001420"
                     /gene_synonym="uppS"
     CDS             14842..15600
                     /gene="ispU"
                     /locus_tag="KLK57_001420"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HBE0891829.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGRIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            15613..16470
                     /gene="cdsA"
                     /locus_tag="KLK57_001421"
     CDS             15613..16470
                     /gene="cdsA"
                     /locus_tag="KLK57_001421"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HBE0891830.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            16482..17834
                     /gene="rseP"
                     /locus_tag="KLK57_001422"
     CDS             16482..17834
                     /gene="rseP"
                     /locus_tag="KLK57_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HBE0891831.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVMALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            17864..20296
                     /gene="bamA"
                     /locus_tag="KLK57_001423"
     CDS             17864..20296
                     /gene="bamA"
                     /locus_tag="KLK57_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HBE0891832.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            20418..20903
                     /gene="skp"
                     /locus_tag="KLK57_001424"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             20418..20903
                     /gene="skp"
                     /locus_tag="KLK57_001424"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HBE0891833.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            20907..21932
                     /gene="lpxD"
                     /locus_tag="KLK57_001425"
     CDS             20907..21932
                     /gene="lpxD"
                     /locus_tag="KLK57_001425"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HBE0891834.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            22037..22492
                     /gene="fabZ"
                     /locus_tag="KLK57_001426"
     CDS             22037..22492
                     /gene="fabZ"
                     /locus_tag="KLK57_001426"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HBE0891835.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            22496..23284
                     /gene="lpxA"
                     /locus_tag="KLK57_001427"
     CDS             22496..23284
                     /gene="lpxA"
                     /locus_tag="KLK57_001427"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HBE0891836.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            23284..24432
                     /gene="lpxB"
                     /locus_tag="KLK57_001428"
     CDS             23284..24432
                     /gene="lpxB"
                     /locus_tag="KLK57_001428"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HBE0891837.1"
                     /translation="MTEQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            24429..25025
                     /gene="rnhB"
                     /locus_tag="KLK57_001429"
     CDS             24429..25025
                     /gene="rnhB"
                     /locus_tag="KLK57_001429"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HBE0891838.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            25062..28544
                     /gene="dnaE"
                     /locus_tag="KLK57_001430"
     CDS             25062..28544
                     /gene="dnaE"
                     /locus_tag="KLK57_001430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HBE0891839.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEAHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            28557..29516
                     /gene="accA"
                     /locus_tag="KLK57_001431"
     CDS             28557..29516
                     /gene="accA"
                     /locus_tag="KLK57_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HBE0891840.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAVSLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
BASE COUNT         7159 a         7120 c         8160 g         7109 t
ORIGIN      
        1 ttgaagatgc ggtagagaaa agaatattta ccgttgagca gctttatcat catttgcacg
       61 aggcgtgggg tcagcatgag aaaggttcgc tcttttcgct agtggttgaa aatgcctggg
      121 aaaaatcacg ctcaaatagt ttaagccgca gtacggaaga tcagtttttt atgtatttac
      181 gggtaaacac cctaaataaa ctggtaccct acgcggcaca acgatttatt gataatctgc
      241 ctgcgatttt cgccggaacg tttaatcatg cattattgga agatgccagc gaatgcagcg
      301 atcttcttaa gctatataaa aatgtcgctg taaaacatgt gtttagccat ccggatgtcg
      361 agcagcttga attgcagggc tatcgggtca ttagcggatt attagagatt tatcgtcctt
      421 tattaagcct gtcgttatca gactttactg aactggtaga aaaagaacgg gtgaaacgtt
      481 tccctattga gtcgcgctta ttccacaaac tctcgacgcg ccatcggctg gcctatgtcg
      541 aggctgtcag taaattaccg tcagattctc ctgagtttcc gctatgggaa tattattacc
      601 gttgccgcct actgcaggat tatatcagcg gtatgaccga cctctatgcg tgggatgaat
      661 accgacgtct gatggccgta gaacaataac caggcttttg taaagacgaa caataaattt
      721 ttaccttttg cagaaacttt agttcggaac ttcgcgctat aaaacgaatc tgaagaacac
      781 agcaattttg cgttatctgt taatcgagac tgaaatacat gaaaaaaacc acattagcac
      841 tgagtgcact ggctctgagt ttaggtttgg cgttatctcc gctctctgca acggcggctg
      901 agacttcttc agcaacgaca gcccagcaga tgccaagcct tgcaccgatg ctcgaaaagg
      961 tgatgccttc agtggtcagc attaacgtag aaggtagcac aaccgttaat acgccgcgta
     1021 tgccgcgtaa tttccagcag ttcttcggtg atgattctcc gttctgccag gaaggttcac
     1081 cgtttcagag ctctccgttc tgccaggggg gccagggcgg taatggcggc ggccagcaac
     1141 agaaattcat ggcgctgggt tccggcgtca tcattgatgc cgataaaggc tatgtcgtca
     1201 ccaacaacca tgttgttgat aacgcgacgg tgattaaagt ccagctgagc gatggtcgta
     1261 agttcgacgc gaagatggtt ggcaaagatc cgcgctctga tatcgcgctg atccaaatcc
     1321 agaacccgaa aaacctgacc gcaattaaga tggcggattc tgatgcactg cgcgtgggtg
     1381 attacaccgt agcgattggt aacccgtttg gtctgggcga gacggtaact tccgggattg
     1441 tctctgcgct ggggcgtagc ggcctgaatg ctgaaaacta cgaaaacttc atccagaccg
     1501 atgcagcgat caaccgtggt aactccggtg gtgcgctggt taacctgaac ggcgaactga
     1561 tcggtatcaa caccgcgatc ctcgcaccgg acggcggcaa catcggtatc ggttttgcta
     1621 tcccgagtaa catggtgaaa aacctgacct cgcagatggt ggaatacggc caggtgaaac
     1681 gcggtgagct ggggattatg gggactgagc tgaactccga actggccaaa gcgatgaaag
     1741 ttgacgccca gcgcggtgct ttcgtaagcc aggttctgcc taattcctcc gctgcaaaag
     1801 cgggcattaa agcgggtgat gtgatcacct cactgaacgg taagccgatc agcagctttg
     1861 ccgcactgcg tgctcaggtg ggtactatgc cggtgggcag caaactgacc ctgggcttac
     1921 tgcgcgacgg taagcaggtt aacgtgaacc tggaactgca gcagagcagc cagaatcagg
     1981 ttgattccag ctccatcttc aacggcattg aaggcgctga gatgagcaac aaaggcaaag
     2041 atcagggcgt ggtagtgaac aacgtgaaaa cgggcactcc ggctgcgcag atcggcctga
     2101 agaaaggtga tgtgattatt ggcgcgaacc agcaggcagt gaaaaacatc gctgaactgc
     2161 gtaaagtgct cgacagcaaa ccgtctgtgc tggcactgaa cattcagcgc ggcgacagca
     2221 ccatctacct gttaatgcag taatctccct caaccccttc ctgaatacgg gaaggggttc
     2281 tccttacaat ctgtgaactt cgccacaact ccatacatct tcatcatcct ttgggcattt
     2341 gcacaatgcc gtacgttacg tacttcctta tgctaagccg tgcataacgg aggacttatg
     2401 gctggctggc atcttgatac caaaatggcg caggatatcg tggcacgtac catgcgcatc
     2461 atcgatacca atatcaacgt aatggatgcc cgcgggcgaa ttatcggcag cggcgatcgt
     2521 gagcgtattg gtgaattgca cgaaggtgca ttgctggtac tttcacaggg acgagtcgtc
     2581 gatatcgatg acgcggtagc acgtcatctg cacggtgtgc ggcaggggat taatctaccg
     2641 ttacggctgg aaggtgaaat tgtcggcgta attggcctga caggtgaacc agagaatctg
     2701 cgtaaatatg gcgaactggt ctgcatgacg gctgaaatga tgctggaaca gtcgcggttg
     2761 atgcacctgt tggcgcagga tagccgtttg cgggaagaac tggtgatgaa cctgattcag
     2821 gctgaggaga atactcccgc acttactgaa tgggcgcaac ggctggggat tgatctcaat
     2881 caaccgcgag tggtggctat tgttgaggtc gacagcggtc agcttggcgt ggacagcgca
     2941 atggcggagt tacaacaact gcaaaatgcg ctgactacgc ccgagcgtaa taatctggtg
     3001 gcaattgttt cgctgaccga aatggtggtg ttgaaaccgg cgttgaactc ttttgggcgc
     3061 tgggatgcag aagatcatcg taagcgagtt gaacaactga ttacccgcat gaaagagtac
     3121 ggccagctgc gttttcgcgt ttcactgggc aactatttta ccggtcctgg cagtattgcc
     3181 cgatcctatc gtacggcgaa aacgacgatg gtggtgggta aacagcggat gccagaaagt
     3241 cgctgctatt tttatcagga tctgatgtta cctgtgttac tcgacagttt gcgtggcgac
     3301 tggcaggcca acgaactggc gcgaccgctg gcgcggctga aagcgatgga caataatggc
     3361 ttgctgcgac gaacgctggc ggcgtggttt cgccacaatg tgcaaccgct ggctacgtca
     3421 aaggcgctgt ttattcatcg taatactctg gagtatcggc ttaatcgtat atcggaactg
     3481 accgggcttg atttgggtaa ttttgatgac aggttgctgc tgtatgtggc gttacaactg
     3541 gatgaagagc ggtaggttat gcgttaaggt cgaggcggcg ctggctcatt cccctcagcc
     3601 cgaagggtag aggggaatgt ccgagttaac cgtcagttcg gcattattta cgcgttagtt
     3661 tttccagatc ggcttcaatc tcgctgatct tattggttac gactgactcc aggtgacgta
     3721 agtcgtcgag gatcttacgc ttaagatcaa cttcgctgcg gtcacgctgg cagatttgat
     3781 caagctcatc aatgatatac cgtagattcg ggctgatttc ctggacttct ttataaccct
     3841 gacccacacc atcagcgacg accgttttac gctgacgcgg atatttaaac ttcacgctct
     3901 tggcgaaaaa ctcgcctttg tctttctgga aatagatttt cagaatatcg ttgttggctt
     3961 cctgccggag gctgtaacga tcaatttctt caggattggt aatacccaga cttttcagat
     4021 tgtcgtacat agcgttacct caaaatgagt cagtaaattg tgcttatttt agcatttggc
     4081 atggcccgcc cggctcgact gtgatcgcag aaagcctttt tcttccagac ttctatcatg
     4141 gcgtaaaaat caaaaaatta cctgctttat tgtggtgata aaattcacgt tctacaccag
     4201 ttccagcatc gttacttccg ggcggcagtt cagacgcaaa ccatacaaac tgcccacgcc
     4261 acgggttgtg tagatgtgtc gttcgccaaa ggcatttaat ccggcgacgt aacgtttatc
     4321 ttcgacaggg gcaaaaggtt caccaaccaa cggtacgcgc agttgcccgc cgtgagtatg
     4381 gccgcacagc atcagatccc agggttcatc acgcatgact tctttgctgt cgggattatg
     4441 tgccagcatc agtcttggca gatttgcttc gctggcggga ggtggtttgc attgtccggc
     4501 ccataagtca ccggtgccta ccagttcgaa ttgcctgttc ggcgtggcga tcaccgtagc
     4561 ctggttaaac aacaccgtga tgcccactga tttcaacgtc tcgccaatta agtgattttt
     4621 ttctgtacca acagggcgat cgtggttgcc gaagcaggca aacgtcggcg cacattcggc
     4681 aaggggggag aggacatcac taaacgccga aaaattcagc ggcatatcaa ataatacgta
     4741 atcgccgccc agtaatatca aatcgggctt ttgttctatg ccaagagcaa tcgcgtcaga
     4801 aatcaggctt aaaggaacaa aacgagagta atggagatcg gccagaaaaa gaattttgaa
     4861 tggtgctgcg ttgtctttaa aaaaggcgag gcggtgacgg attaattcaa accagcctag
     4921 ctcccagtaa tgcatataac cgaaacctga gctcgtggct atcgtcgcgg cagtagcctg
     4981 caacaatcgg cggcgtgaaa tcatcgctca tccctgcaaa aaaaatcggg cagcgtcgtg
     5041 ctgcccgtgt gcatactttt agtcgatggt acgcagcagt tcgttaatgc cgactttgcc
     5101 gcgagttttc gcgtcaactt tcttaacgat aaccgcacag tagaggctgt atttgccatc
     5161 ttttgacggc agattacctg aaacaaccac agaccccgcc ggaacgcgac cgtagtggat
     5221 ttcgccggtt tcacggtcgt aaatacgggt gctctgacca atgtatacgc ccatggaaat
     5281 gacggaacct tcttcgacaa tcaccccttc aaccacttca gagcgtgcgc caatgaagca
     5341 gttatcttca atgatggttg ggttagcctg cagcggttcc agcacgccgc cgatgcccac
     5401 gccaccggaa aggtggacgt ttttaccaat ctgcgcacaa gaaccgacgg tcgcccaggt
     5461 atcaaccatg gtgccttcat caacatatgc gccgatgttg acgtaagacg gcatcagcac
     5521 ggtgttacgg gcaataaacg caccctgacg taccgccgct ggtggcacaa cgcggaagcc
     5581 ttctttctgg aaacgtgctt cgtcgtagtc ggcgaatttc atcggcactt tgtcgaagta
     5641 gcggctttct gccccttcga tcacctgatt atcattaata cggaaagaga gcagcaccgc
     5701 ttttttcaac cactgatgcg tcacccactg accgtcaatt ttttccgcta cacgcagtgc
     5761 accggaatcc agcagggcga tcacctgatt taccgcttcg cgggtaacgg tgtctgcgtt
     5821 ggctggcgtg atctcggcac ggcgttcaaa agcggtttca ataatgttct gtaactgctg
     5881 cattgttaaa ctcttttcat atcagtaaac acatcaccct ttatcgtttg gattgagggc
     5941 ctctgtcaac cgctgatgca cttcctgctg caactcgtta ttaagtgcac gccggtcagc
     6001 ggtggcaatt atgaataaat cttctactcg ctcgccaatg gttgtaattc gggcaccatg
     6061 aagcgaaatt cccagatcgg caaaaatttt cccgactctc gccagcagtc ctggctggtc
     6121 gagcgcgata agctcaagga acgatttgcg gtcggtatgg gttggcaaaa acgtcacttc
     6181 ggtttcaaca gtaaagtgac gtagtttagc cggttgacga cgcggctgtg gtggctgcca
     6241 gctactttgt gtcagtacct gctccagacc aaaccgaata acctcatgac gatctgcgga
     6301 tagcgggcta ccatcgggtt ccagcacgat gaaggtatcc atcgccatac cgtcgcgagt
     6361 ggtgaaaatt tgtgcgtcgt gaacacttaa attgcggcgg tctaattcgg cacagacggc
     6421 ggcaaacaga taggggcggt ccgggctcca gataaaaatc tcggtgcctc cacgcgtagc
     6481 ctgcgggcta agcaatacca gcggtttgct taaatcatgc tgtaataaat ggcgggcatg
     6541 ccaggccagt tgatttgggc tatggcggac aaaatagtta gcacgacagc gtgaccaaat
     6601 ttggtgcagc gcctcttcgt cgatgttatc catgcgcagt agtgccagtg cctggagttg
     6661 gtgatggcga acccgttcgc gcatatccgg cgtgttttgc attccgcgtc gtagctgctt
     6721 ttcggtggca aaatagagtt cgcgcaacag actttgcttc cagctattcc acagcgtttc
     6781 gttggtggcg caaatgtcag ccactgtcag gcataccaga tagtgcagac gattttccgt
     6841 ttgaacttct tcggcaaact gcttgatgac ttcagggtcc tgaatatcgc ggcgttgtgc
     6901 tgtcaccgac atcagcaagt gctggcgaac cagccaggca accagctgtg tttcgcgtga
     6961 gttcagcccg tggagttctg caaaatgcac tacatcctga gcaccgagaa tggagtggtc
     7021 gccgccgcgt cctttggcga tatcgtgaaa cagcgcggcg atgaaaatca gctcagttga
     7081 cggcaggcgc ggccagacgt ccacacacaa cggatggcgc tggcgcgttt cttcactggc
     7141 aaaactctcc agtttcagca tcacgcggat agtatgttca tccaccgtgt aggcgtggaa
     7201 cagatcaaac tgcatctgcc cgacgatatg cgaccattgc ggcatatacg cgccgagcac
     7261 gctatggcga tgcattggca atagcccgcg ccgcaccgct ccggggtgac gcagaatgct
     7321 caaaaacagt tttcgtgctt ccggaatatt acacagcggt tgttgcagat ggcgacgggc
     7381 atggcgtaac tggcgcagcg tggtggagta aatgccggtg atcgcactgt tgcgcaccat
     7441 ggtgtagaac atacgcaaga tggcttccgg ctggcgcata aatagtgttt catcacgcag
     7501 gtcgattagc gtaccgcgta gctgaaactc atcgtcgatt ggacgtggtt tttcgtcggc
     7561 gggaagggcg aggatggctt catcgaacag ttgcagcagc atctggttga gttcactgac
     7621 gcggcgtgta acgcggaagt aatccttcat catccgctcg accggttcgt tgccttcacc
     7681 actgtaattc agacgctgag cgacgctaag ttggcgatcg aataacaggc gattatcgta
     7741 acggctgacg accagatgca gggcaaagcg aatacgccac agtatatgca gacattcgtt
     7801 taattccgcc cgctccgctg aggttaagaa gccaaaccca accatttcat ccagcgatgt
     7861 tgcgccaaaa tgacggcggg ccacccattg cagagtgtgg atatcgcgca agccgccagg
     7921 gctacttttg atgtctggtt caagattgta gctggtgccg tggtaacgct gatggcgctg
     7981 gttctgttct tcaactttcg ccgcgtagaa cttgtcggaa ggccagaatc cttcgctaaa
     8041 aatatgtttt tgcagttcga ggaagagcgc aacatcgcca attaataagc gggattcgat
     8101 taaattggtg gcgacggtta aatccgataa cccttccagc atgcactctt caagcgtgcg
     8161 cacactatga ccgacttcca gctttacatc ccagagcagc gttaacagct cgcccacttt
     8221 ttgcgcctga tcgtccggga gctttttacg gcttaaaatc agtaaatcga tgtcagaaag
     8281 tggatgcagc tcgccacgac cgtagccacc gacggcgacc aatgccaggt cggcaatctg
     8341 gctgaatccc gcttcaatcc ataatcgttg caggagctgg tcgataaact cggtgcgcgc
     8401 ctcaatcaac tgttctgcag agatcccatt gtcaaaggca tcacccagcc aacgctggaa
     8461 agtatcgata tgggctttta tcccaccgac ggttaattca tcacggggcc agacgcatgg
     8521 attttgcggt tgaccgggca gggtggggag agcggtgttt gcgtactgtt ctggaagggt
     8581 attcattgtg cgccacccat aagattaaat tatcgcatta aaaaagccgg cattcgccgg
     8641 cttcatctta ttcgtcgtgc gagattatcg ccgggatggt gtcatccttg cgtagcgtca
     8701 gaatttcgca gccgttatca gtcaccacaa tagtatgctc atattgtgca gacaagctgc
     8761 gatctttggt ttttaccgtc cagccatctt tcatggtgcg gatctctttt ttacccgcgt
     8821 tgaccattgg ctcgatggtg aacgtcatcc caggtttcag tacgacgttg gtttcacggg
     8881 agtcatagtg cagcacctgc ggttcttcat ggaagccgcg accaataccg tgtccgcaat
     8941 attcacgaac gacggagaag ccttctgctt cgataaactt ctgaatcgcc gcaccgattt
     9001 cgcgcaggtt aatgcctggt ttgaccatgc gcagcgccag gtacaggctt tcttgcgtga
     9061 tgcggcacag acgttcgccc atgatggtcg gcttaccgac gataaacatt ttcgaggtat
     9121 cgccgtggaa accatctttg attacggtga catcaatgtt aacgatatcg ccatctttca
     9181 gcagcttcgc atcgtccggg ataccgtggc acaccacttc attaatagag atgcaaacgg
     9241 atttcggata gccgtgatag ccgaggcagg cagaaaccgc gtgttgttca ttaacaatgt
     9301 aatcattaca gatgcgatcc agctcgccgg tgctgacgcc cggtttaaca tacggttcga
     9361 tcatctccag cacttcggca gccagtcggc cagcgacgcg cattttttcg atatcttctg
     9421 gggtcttgat tgagatagcc attaattctg tccatcagcg tcggtgatac cgacaatata
     9481 tatgtaagtg ccgtcaatgg tatcacaccc gggcaaattg agaatcattc tgaatttcgc
     9541 caaacgtgcc actgaacgtt ttctataata gaaaattcaa cgtctgatgc tgtacacagc
     9601 gccaacaatt attggtgtcc atgacgtatt tgtggtataa agcgcgccgg acttccgatc
     9661 catttcgtat acacagactg gacggaagcg acaatctcac tttgtgtaac aacacacacg
     9721 tatcggcaca tattccgggg tgccctttgg ggtcggtaat atgggatacg tggaggcata
     9781 accccaactt ttatatagag gttttaatca tggcaactgt ttccatgcgc gacatgctca
     9841 aggctggtgt tcacttcggt caccagaccc gttactggaa cccgaaaatg aagccgttca
     9901 tcttcggtgc gcgtaacaaa gttcacatca tcaaccttga gaaaactgta ccgatgttca
     9961 acgaagctct ggctgaactg aacaagattg cttctcgcaa aggtaaaatc cttttcgttg
    10021 gtactaaacg cgctgcaagc gaagcggtga aagacgctgc tctgagctgc gaccagttct
    10081 tcgtgaacca tcgctggctg ggcggtatgc tgactaactg gaaaaccgtt cgtcagtcca
    10141 tcaaacgtct gaaagacctg gaaactcagt ctcaggacgg tactttcgaa aagctgacca
    10201 agaaagaagc gctgatgcgc actcgtgagc tggagaaact ggaaaacagc ctgggcggta
    10261 tcaaagacat gggcggtctg ccggacgctc tgtttgtaat cgatgctgac cacgaacaca
    10321 ttgctatcaa agaagcaaac aacctgggta ttccggtatt tgctatcgtt gataccaact
    10381 ctgatccgga cggtgttgac ttcgttatcc cgggtaacga cgacgcaatc cgtgctgtga
    10441 ccctgtacct gggcgctgtt gctgcaaccg tacgtgaagg ccgttctcag gatctggctt
    10501 cccaggcgga agaaagcttc gtagaagctg agtaataagg cttgataact cccccaaaat
    10561 agttcgagtt gcagaaaggc ggcaagctcg agaattcccg ggagcttaca tcagtaagtg
    10621 accgggatga gcgagcgaag acaacgcatc tgcggcgcga aatatgaagg gggagagccc
    10681 ttatagacca ggtagtacac gtttggttag ggggcctgca tatggccccc tttttcactt
    10741 ttatatctgt gcggtttaat gccgggcaga tcacatctcc gaggatttta gaatggctga
    10801 aattaccgca tccctggtaa aagagctgcg tgagcgtact ggcgcaggca tgatggattg
    10861 caaaaaagca ctgactgaag ctaacggcga catcgagctg gcaatcgaaa acatgcgtaa
    10921 gtccggtgct attaaagcag cgaaaaaagc aggcaacgtt gctgctgacg gcgtgatcaa
    10981 aaccaaaatc gacggcaact acggcatcat tctggaagtt aactgccaga ctgacttcgt
    11041 tgcaaaagac gctggtttcc aggcgttcgc agacaaagtt ctggacgcag ctgttgctgg
    11101 caaaatcact gacgttgaag ttctgaaagc acagttcgaa gaagaacgtg ttgcgctggt
    11161 agcgaaaatt ggtgagaaca tcaacattcg ccgcgttgct gcgctggaag gcgacgttct
    11221 gggttcttat cagcacggtg cgcgtatcgg cgttctggtt gctgctaaag gcgctgacga
    11281 agagctggtt aaacacatcg ctatgcacgt tgctgcaagc aagccagaat tcatcaaacc
    11341 agaagacgta tccgctgaag tggtagaaaa agaataccag gtacagctgg atatcgcgat
    11401 gcagtctggt aagccgaaag aaatcgcaga gaaaatggtt gaaggccgca tgaagaaatt
    11461 caccggcgaa gtttctctga ccggtcagcc gttcgttatg gaaccaagca aaactgttgg
    11521 tcagctgctg aaagagcata acgctgaagt gactggcttc atccgcttcg aagtggggga
    11581 aggcatcgag aaagttgaga ctgactttgc agcagaagtt gctgcgatgt ccaagcagtc
    11641 ttaattatcg aaaaggagcc gcctgagggc ggcttctttt tgtgcccatc ttgtaaattc
    11701 agctaaccct tgtggggctg cgctgaaaag cgacgtacaa tgtcgctagt attaattcat
    11761 ttcaatcgtt gacagtctca ggaaagaaac atggctacca atgcaaaacc cgtctataaa
    11821 cgcattctgc ttaagttgag tggcgaagct ctgcagggca ctgaaggctt cggtattgat
    11881 gcaagcatac tggatcgtat ggctcaggaa atcaaagaac tggttgaact gggtattcag
    11941 gttggtgtgg tgattggtgg gggtaacctg ttccgtggcg ctggtctggc gaaagcgggt
    12001 atgaaccgcg ttgtgggcga ccacatgggg atgctggcga ccgtaatgaa cggcctggca
    12061 atgcgtgatg cactgcaccg cgcctatgtg aacgctcgtc tgatgtccgc tattccattg
    12121 aatggcgtgt gcgacagcta cagctgggca gaagctatca gcctgttgcg caacaaccgt
    12181 gtggtgatcc tctccgccgg tacaggtaac ccgttcttta ccaccgactc agcagcttgc
    12241 ctgcgtggta tcgaaattga agccgatgtg gtgctgaaag caaccaaagt tgacggcgtg
    12301 tttaccgctg atccggcgaa agatccaacc gcaaccatgt acgagcaact gacttacagc
    12361 gaagtgctgg aaaaagagct gaaagtcatg gacctggcgg ccttcacgct ggctcgtgac
    12421 cataaattac cgattcgtgt tttcaatatg aacaaaccgg gtgcgctgcg ccgtgtggta
    12481 atgggtgaaa aagaagggac tttaatcacg gaataattcc cgtgatggat aaataagggt
    12541 aagattccgc gtaagtatcg cgggggcgta agtctggtta taaggcgtta ttgttgcagg
    12601 cagtttggtc acggccagcg cgcagcaacc ggagcgtaca aaagtacgtg aggatggcga
    12661 gcactgctcg gggccaaaat ggcaaataaa atagcctaat aatccagacg attacccgta
    12721 atatgtttaa tcagggctat acttagcaca cttccactgt gtgtgactgt ctggtctgac
    12781 tgagacaagt tttcaaggat tcgtaacgtg attagcgata tcagaaaaga tgctgaagta
    12841 cgcatggaca aatgcgtaga agcgttcaaa acccaaatca gcaaaatacg cacgggtcgt
    12901 gcttctccca gcctgctgga tggcattgtc gtggaatatt acggcacgcc gacgccgctg
    12961 cgtcagctgg caagcgtaac ggtagaagat tcccgtactc tgaaaatcaa cgtgtttgat
    13021 cgttcaatgt ctccggccgt tgaaaaagcg attatggcgt ccgatcttgg cctgaacccg
    13081 aactctgcgg gtagcgacat ccgtgttccg ctgccgccgc tgacggaaga acgtcgtaaa
    13141 gatctgacca aaatcgttcg tggtgaagca gaacaagcgc gtgttgcagt acgtaacgtg
    13201 cgtcgtgacg cgaacgacaa agtgaaagca ctgttgaaag ataaagagat cagcgaagac
    13261 gacgatcgcc gttctcagga cgatgtacag aaactgactg atgctgcaat caagaaaatt
    13321 gaagcggcgc tggcagacaa agaagcagaa ctgatgcagt tctgatttct tgaacgacaa
    13381 aaacgccgct cagtagatcc ttgcggatcg gctggcggcg ttttgctttt tattctgtct
    13441 caactctgga tgtttcatga agcaactcac cattctgggc tcgaccggct cgattggttg
    13501 cagcacgctg gacgtggtgc gccataatcc cgaacacttc cgcgtagttg cgctggtggc
    13561 aggcaaaaat gtcactcgca tggtagaaca gtgcctggaa ttctctcccc gctatgccgt
    13621 aatggacgat gaagcgagtg cgaaacttct taaaacgatg ctacagcaac agggtagccg
    13681 caccgaagtc ttaagtgggc aacaagccgc ttgcgatatg gcagcgcttg aggatgttga
    13741 tcaggtgatg gcagccattg ttggcgctgc tgggctgtta cctacgcttg ctgcgatccg
    13801 cgcgggtaaa accattttgc tggccaataa agaatcactg gttacctgcg gacgtctgtt
    13861 tatggacgcc gtaaagcaga gcaaagcgca attgttaccg gtcgatagcg aacataacgc
    13921 catttttcag agtttaccgc aacctatcca gcataatctg ggatacgctg accttgagca
    13981 aaatggcgtg gtgtccattt tacttaccgg gtctggtggc cctttccgtg agacgccatt
    14041 gcgcgatttg gcaacaatga cgcccgatca agcctgccgt catccgaact ggtcgatggg
    14101 acgtaaaatt tctgtcgatt cggctaccat gatgaacaaa ggtctggaat acattgaagc
    14161 gcgttggctg tttaacgcca gcgccagcca gatggaagtg ctgattcacc cgcagtcagt
    14221 gattcactca atggtgcgct atcaggacgg cagtgttctg gcgcagctgg gggaaccgga
    14281 tatgcgtacg ccaattgccc acaccatggc atggccgaat cgcgtgaact ctggcgtgaa
    14341 gccgctcgat ttttgcaaac taagtgcgtt gacatttgcc gcaccggatt atgatcgtta
    14401 tccatgcctg aaactggcga tggaggcgtt cgaacaaggt caggcagcga cgacagcatt
    14461 gaatgccgca aacgaaatca ccgttgctgc ttttcttgcg caacaaatcc gctttacgga
    14521 tatcgctgcg ttgaatttat ctgtactgga aaaaatggat atgcgcgaac cacaatgtgt
    14581 ggacgatgtg ttatctgttg atgcgaacgc gcgtgaagtc gccagaaaag aggtgatgcg
    14641 tctcgcaagc tgaggataat ccggctacag agagtcgcgc tatttgttag cgtagggctt
    14701 cagtgatata gtctgcgcca tctgatcgta agtagttggc tttataaggt caggtatgcc
    14761 gtggttttac acggcttttt tttgtatagg cttcagtatt cctgagtacc gtaaaccctg
    14821 tcagggaata aaaaacgcgt gatgttgtct gctactcaac cacttagcga aaaattgcca
    14881 gcgcatggct gccgtcatgt tgcgatcatt atggacggca atggtcgctg ggcaaaaaag
    14941 caagggagga ttcgtgcctt tgggcataaa gccggggcaa aatccgtccg ccgggctgtc
    15001 tcttttgcgg ccaacaacgg tattgaggcg ttaacgctgt atgcctttag tagtgaaaac
    15061 tggaaccgac cagcgcagga agtcagtgcg ttaatggaac tgtttgtgtg ggcgctcgat
    15121 agcgaagtaa aaagtctgca ccgacataac gtgcgtctgc gtattattgg cgataccagt
    15181 cgctttaact cgcgtttgca agaacgtatt cgtaaatctg aagcgctaac agctgggaat
    15241 accggtctga cgctgaatat tgcggcgaac tacggtggac gttgggatat agtccaggga
    15301 gtcaggcaac tggctgaaaa ggtgcagcaa ggaaacctgc aaccagatca gatagatgaa
    15361 gagatgctaa accagcatgt ctgtatgcat gaactggccc ctgtagattt agtaattagg
    15421 actggggggg agcatcgcat tagtaacttt ttgctttggc aaattgccta tgccgaactt
    15481 tactttacag atgttctctg gcctgatttc gatgaacaag actttgaagg ggcgttaaat
    15541 gcctttgcta atcgagagcg tcgtttcggc ggcaccgagc ccggtgatga aacagcctga
    15601 tggggggcgc ttttgctgaa gtatcgcctg atatctgctt ttgtgttaat acccgtcgtc
    15661 atcgcggcgt tgtttctgtt gccgccggtg gggttcgcca tcgtaacgct ggtggtctgc
    15721 atgctggcag cgtgggaatg gggacagctt agcggtttta ccactcgttc gcagcgagta
    15781 tggttggcgg tgttatgcgg gttattgttg gcgctgatgc tttttctgtt gccggaatat
    15841 caccgaaata ttcatcaacc gctggttgaa atctcacttt gggcttcgtt gggttggtgg
    15901 attgtcgcgc tattgctggt gctgttttac ccaggttccg cagcaatctg gcgtaactct
    15961 aaaacattgc gccttatttt tggcgtgcta accattgttc ccttcttctg gggcatgctg
    16021 gcgttacggg cctggcacta tgacgagaat cattacagtg gcgcaatatg gctgctctat
    16081 gtcatgatcc tggtatgggg cgctgactcc ggcgcatata tgtttggcaa attgtttggt
    16141 aaacataagc tggcaccgaa ggtttctccg ggtaaaacct ggcaaggctt tataggtgga
    16201 ctagcgaccg cagcggtaat ctcatggggt tatggcatgt gggcgaatct cgacgtcgct
    16261 cccgtcacct tactcatttg ctctattgtc gcagcgttag cctcagtgct cggcgatctg
    16321 accgagagta tgtttaagcg tgaagcagga attaaggaca gcggtcattt aattccagga
    16381 cacggtggta ttttagatcg tattgatagc ctgacggctg cggtaccggt ctttgcttgc
    16441 ttgttgttac tggtattcag gacgctttaa cggaaggtaa tatgctgagt tttctctggg
    16501 atttggcttc gttcatcgtt gcactgggtg tacttatcac cgtgcatgaa tttggtcatt
    16561 tctgggttgc ccggcgttgt ggtgttcgcg ttgagcgttt ctcaataggg tttggtaagg
    16621 cgctctggcg gcgaactgat aagctcggca ccgaatatgt tatggccctg attccgctgg
    16681 gcggttatgt caaaatgctg gatgagcgcg cagaaccggt cgttccggaa ctccgccacc
    16741 atgccttcaa taataaatct gttggccaac gagcggcgat tattgccgca ggtccggttg
    16801 caaacttcat ttttgctatc tttgcctact ggctggtttt tattattggt gtgcctggcg
    16861 tacgtccggt ggttggtgaa atagcagcca attcgatagc tgcggaagca caaattgcac
    16921 caggtacgga actaaaagcc gtagatggta tcgaaacgcc tgattgggat gccgtgcgtt
    16981 tgcagttggt cgataaaatt ggcgatgaaa gcaccaccat tacagtagcg ccatttggca
    17041 gcgaccaacg gcgggatgta aagctcgatt tacgtcactg ggcgtttgag cctgataaag
    17101 aagatccggt atcttcgctg gggattcgtc ctcgtgggcc gcaaattgaa cctgtactgg
    17161 aaaatgtgca gccaaactcg gcggcaagca aggcaggttt gcaagcaggc gacaggatcg
    17221 ttaaagtcga tggtcagccc ttaacgcagt gggtgacctt tgtgatgctt gtccgggata
    17281 acccgggtaa atccttagcg ttagaaatcg aaaggcaggg gagtcccttg tctttgacat
    17341 taatcccgga gagtaaaccg ggtaatggta aagcgattgg ttttgtcggt attgagccga
    17401 aagtcattcc tttgccagat gagtataaag ttgtacgcca gtatgggccg ttcaacgcca
    17461 tcgtcgaagc cacggacaaa acgtggcagc tgatgaagct gacggtcagt atgctgggaa
    17521 aattgatcac cggtgatgtg aaactgaaca acctcagtgg gccgatctct atcgccaagg
    17581 gggctgggat gacagcggaa ctcggggtag tttattacct gccgtttctt gcgcttatta
    17641 gcgtgaactt agggataatt aacctgtttc cgttgcccgt acttgacggg gggcatctgc
    17701 tgttccttgc gatcgaaaag atcaagggcg gaccggtatc cgagcgggtt caagactttt
    17761 gttatcgcat tggctcgatt ctgctggtgc tgttaatggg gcttgcactt ttcaatgatt
    17821 tctctcggtt atgagagtta gttaggaaga acgcataata acgatggcga tgaaaaagtt
    17881 gctcatagcg tcgctgctgt ttagcagcgc caccgtatac ggtgctgaag ggttcgtagt
    17941 gaaagatatt catttcgaag gccttcagcg tgtcgccgtt ggtgcggcac tcctcagtat
    18001 gccggtgcgc acaggcgaca cggttaatga tgaagatatc agtaatacca ttcgcgctct
    18061 gtttgctacc ggcaactttg aggatgttcg cgtccttcgt gatggtgata cccttctggt
    18121 tcaggtaaaa gaacgtccga ccattgccag cattactttc tccggtaaca aatcggtgaa
    18181 agatgacatg ctgaagcaaa acctcgaggc ttctggtgtg cgtgtgggcg aatccctcga
    18241 tcgcaccacc attgccgata tcgagaaagg tctggaagac ttctactaca gcgtcggtaa
    18301 atatagcgcc agcgtaaaag ctgtcgtgac cccgctgccg cgcaaccgtg ttgacctaaa
    18361 actggtgttc caggaaggtg tgtcagctga aatccagcaa attaacattg ttggtaacca
    18421 tgctttcacc accgacgaac tgatctctca tttccaactg cgtgacgaag tgccgtggtg
    18481 gaacgtggta ggcgatcgta aataccagaa acagaaactg gcgggcgacc ttgaaaccct
    18541 gcgcagctac tatctggatc gcggttatgc ccgtttcaac atcgactcta cccaggtcag
    18601 tctgacgcca gataaaaaag gtatttacgt cacggtgaac attaccgaag gcgatcagta
    18661 caagctttct ggcgttgaag tgagcggcaa ccttgccggg cactccgctg aaattgagca
    18721 gctgactaag atcgagccgg gcgagctgta taacggcacc aaagtgacca agatggaaga
    18781 tgacatcaaa aagcttctcg gtcgctatgg ttatgcctat ccgcgcgtac agtcgatgcc
    18841 tgaaattaac gatgccgaca aaaccgttaa attacgcgtg aacgttgatg cgggtaaccg
    18901 tttctacgtg cgtaagatcc gttttgaagg taacgatacc tcgaaagatg ccgtcctgcg
    18961 tcgcgaaatg cgtcagatgg aaggtgcatg gctgggaagc gatctggtcg atcagggtaa
    19021 ggagcgtctg aatcgtctgg gcttctttga aactgtcgat accgataccc aacgtgttcc
    19081 gggtagcccg gaccaggtag atgtagtcta caaggtgaaa gaacgtaaca ccggtagctt
    19141 caacttcggg attggttacg gtactgaaag tggcgtgagc ttccaggctg gcgtgcaaca
    19201 ggataactgg ttaggtacag gttatgctgt tggtatcaac gggaccaaaa acgattacca
    19261 gacctatgct gaattgtcgg taaccaaccc gtacttcact gtagatggtg taagcctcgg
    19321 tggtcgtctc ttctataatg acttccaggc agatgacgcc gacctgtccg actataccaa
    19381 caagagttat ggtacagacg tgacgttggg cttcccgatc aacgaatata actcgctgcg
    19441 tgcaggtctg ggttatgtac ataactccct gtccaacatg cagcctcagg ttgcgatgtg
    19501 gcgttatctg tactctatgg gtgaacatcc gagcacctct gatcaggata acagcttcaa
    19561 aacggacgac ttcacgttca actatggttg gacctataac aagcttgacc gtggttactt
    19621 cccgacagat ggttcacgtg tcaacctgac cggtaaagtg accattcctg gatcggataa
    19681 cgaatactac aaagtgacgt tagacacggc gacttatgtg ccgatcgatg acgatcacaa
    19741 atgggttgtt ctggggcgta cccgctgggg ttatggtgat ggtttaggcg gcaaagagat
    19801 gccgttctac gagaacttct atgccggtgg ttccagcacc gtgcgtggct tccagtccaa
    19861 tacaattggt ccgaaagcag tttacttccc gcatcaggcc agtaattatg atccggacta
    19921 tgattacgaa tgtgcgactc aggacggcgc gaaagacctg tgtaaatcgg atgatgctgt
    19981 aggcggtaac gccatggcgg ttgccagcct cgagttcatc accccgacgc cgtttattag
    20041 cgataagtat gctaactcgg ttcgtacttc cttcttctgg gatatgggta ccgtttggga
    20101 tacaaactgg gattccagcc aatattctgg atatccggac tatagtgatc caagcaatat
    20161 ccgtatgtct gcgggtatcg cattacaatg gatgtcccca ttggggccgt tggtgttctc
    20221 ctacgcccag ccgttcaaaa agtacgatgg agacaaggca gaacagttcc agtttaacat
    20281 cggtaaaacc tggtaagtgt tctccacaaa ggaatgtagt ggtagtgtag cgatgacttt
    20341 aggcgatcaa tataagatcg ccgggccacg caaagaactg caccctccgg tgcaaatggg
    20401 atggtaagga gtttattgtg aaaaagtggt tattagcggc aggtctcggt ttagcactgg
    20461 caacttctgc tcaggcggct gacaaaattg caatcgtcaa tatgggcagc ctgttccagc
    20521 aggtagcgca gaaaaccggt gtttctaaca cgctggaaaa tgagttcaaa ggccgtgcca
    20581 gcgaactgca gcgtatggaa accgatctgc aggctaaaat gaaaaagctg cagtccatga
    20641 aagcgggcag cgatcgcact aagctggaaa aagacgtgat ggctcagcgc cagacttttg
    20701 ctcagaaagc gcaggctttt gagcaggatc gcgcacgtcg ttccaacgaa gaacgcggca
    20761 aactggttac tcgtatccag actgctgtga aatccgttgc caacagccag gatatcgatc
    20821 tggttgttga tgcaaacgcc gttgcttaca acagcagcga tgtaaaagac atcactgccg
    20881 acgtactgaa acaggttaaa taagtaatgc cttcaattcg actggctgat ttagcgcagc
    20941 agttggatgc agaactacac ggtgatggcg atatcgtcat caccggcgtt gcgtccatgc
    21001 aatctgcaca aacaggtcac attacgttca tggttaaccc aaaataccgt gagcatttag
    21061 gcttgtgcca ggcgtccgcg gttgtcatga cccaggacga tcttcctttc gcgaaaagtg
    21121 ccgcgctggt agtgaagaat ccctacctga cttacgcgcg catggcgcaa attttagata
    21181 ccacgccgca gcccgcgcag aacattgcac ccagtgcggt gatcgacgcg acggcgaagc
    21241 tgggtaacaa cgtatcgatt ggcgctaacg cggtgattga gtccggcgtt gaactgggcg
    21301 ataacgtgat tatcggtgcc ggttgcttcg taggtaaaaa cagcaaaatc ggtgcaggtt
    21361 cgcgtctctg ggcgaacgta accatttacc atgagatcca gatcggtcag aattgcctga
    21421 tccagtccgg aacagtggta ggcgcagacg gctttggtta tgccaacgat cgtggtaact
    21481 gggtgaagat cccacagatt ggtcgcgtaa ttattggcga tcgcgtggag atcggtgcct
    21541 gcacaaccat cgatcgcggc gcgctggatg acactgttat tggcaatggc gtgatcattg
    21601 ataaccagtg ccagattgca cataacgtcg tgattggcga caatacggcg gttgccggtg
    21661 gcgtcattat ggcgggcagc ctgaaaattg gtcgttactg catgatcggc ggagccagcg
    21721 taatcaacgg gcatatggaa atatgcgaca aagtgacggt tacgggcatg ggtatggtga
    21781 tgcgtcccat cactgaacca ggcgtctatt cctcaggcat tccgctgcaa cccaacaaag
    21841 tctggcgcaa aaccgctgca ctggtgatga acattgatga catgagcaag cgtctgaaat
    21901 cgcttgagcg caaggttaat caacaagact aacgttccat cttttgttcg ccaaacttta
    21961 cggcctgtct cattcttacg attgcggcag gccgtgttat tattgtcgtt tcttatattt
    22021 tgacaggaag agtatcttga ctactaacac tcatactctg cagattgaag agattttaga
    22081 acttctgccg caccgtttcc cgttcttact ggtggatcgc gtgctggatt ttgaagaagg
    22141 tcgttttctg cgcgcagtaa aaaatgtctc tgtcaatgag ccattcttcc agggccattt
    22201 ccctggaaaa ccgattttcc cgggtgtgct gattctggaa gcaatggcac aggcaacagg
    22261 tattctggcg tttaaaagcg taggaaaact ggaaccgggt gagctgtact acttcgctgg
    22321 tattgacgaa gcgcgcttca agcgcccggt cgtgcctggc gatcaaatga tcatggaagt
    22381 cactttcgaa aaaacgcgcc gcggcctgac ccgttttaaa ggggttgctc tggtcgatgg
    22441 taaagtagtt tgcgaagcaa cgatgatgtg tgctcgtagc cgggaggcct gatacgtgat
    22501 tgataaatcc gcctttgtgc atccaaccgc cattgtggaa gagggcgcgt caattggcgc
    22561 gaacgcacac attggtcctt tttgtatcgt tggaccccat gtcgaaattg gtgagggtac
    22621 cgtactgaaa tctcacgttg tcgtgaatgg tcatactaaa attggccgcg ataatgagat
    22681 ttatcagttc gcctccatcg gcgaagttaa ccaggatctg aaatatgctg gcgaaccgac
    22741 ccgtgtggaa atcggcgatc gtaaccgcat tcgcgaaagc gtcaccattc atcgtggcac
    22801 agtccagggc ggtggattga cgaaggtggg cagcgacaac ttactgatga tcaacgcgca
    22861 cattgcgcac gattgtacgg taggtaaccg ctgtattctc gccaacaacg caacgctggc
    22921 gggtcacgta tcggttgacg acttcgcgat catcggcggc atgaccgcag tccatcagtt
    22981 ctgcatcatt ggtgcgcacg tgatggttgg cggctgctcc ggtgtggcgc aggacgtccc
    23041 tccttatgtc attgcgcagg gtaaccacgc aacgccgttc ggtgtcaata tcgaagggct
    23101 gaagcgccgc ggattcagcc gtgaggcgat taccgctatc cgcaatgcgt ataagctgat
    23161 ttatcgtagc ggtaaaacgc tcgatgaagt gaaaccggaa attgctgaac tggcggaaac
    23221 atatccggaa gtgaaagcct ttaccgattt ctttgcacgc tcaacgcgcg gtctgattcg
    23281 ttaatgactg aacagcgtcc attaacgatt gccctggtcg ccggagaaac ctccggcgat
    23341 atcctggggg ccggtttaat ccgcgctctg aaagaacatg tgcccaacgc ccgctttgtt
    23401 ggtgttgccg ggccacgaat gcaggctgaa ggctgcgaag cttggtacga aatggaagaa
    23461 ctggcggtaa tgggcattgt tgaagtgctc ggtcgtctgc gtcgcttact gcatattcgt
    23521 gccgatctga caaagcgttt tggcgaactg aagccagatg tttttgttgg tattgatgcg
    23581 cccgacttca atattactct tgaaggtaac ctcaaaaagc agggtatcaa aaccattcat
    23641 tacgtcagtc catccgtctg ggcgtggcga cagaaacgcg ttttcaaaat aggcagagcc
    23701 accgatctgg tgctcgcatt tctgcctttc gaaaaagcgt tttatgacaa atacaatgta
    23761 ccgtgccgct ttatcggtca taccatggct gatgccatgc cgttagatcc agataaaaat
    23821 gccgcccgtg atgtgctggg gatccctcac gatgcccact gcctggcgtt gctaccgggg
    23881 agccgtggtg cagaagttga aatgcttagt gccgatttcc tgaaaacggc ccagcttttg
    23941 cgccagacat atccggatct cgaaatcgtg gtgccactgg tgaatgccaa acgccgcgag
    24001 cagtttgaac gcatcaaagc tgaagtcgcg ccagaccttt cagttcattt gctggatggg
    24061 atgggccgtg aggcgatggt cgccagcgat gcggcgctac tggcgtcggg tacggcagcc
    24121 ctggagtgta tgctggcgaa atgcccgatg gtggtgggat atcgcatgaa gccttttacc
    24181 ttctggttgg cgaagcggct ggtgaaaact gattatgtct cgctgccaaa tctgctggcg
    24241 ggcagagagt tagttaaaga attattgcag gaagagtgtg agccgcaaaa actggctgcg
    24301 gcgctgttac cgctgttggc gaacgggaaa accagccacg cgatgcacga taccttccgt
    24361 gaactgcatc agcagatccg ctgcaatgcc gatgagcagg cggcacaagc cgttctggag
    24421 ttagcacaat gatcgaattt gtttatccgc acacgcagct ggttgcgggt gtggatgaag
    24481 tcggacgcgg gccgttagtt ggcgcggtcg tcaccgctgc ggtgatcctt gacccggcgc
    24541 gcccgattgc cgggctgaat gattccaaaa agctgagcga aaaacgccgt ctggcgctct
    24601 atgaagagat caaagagaaa gcgttgagct ggagtctggg ccgcgcggaa ccccacgaaa
    24661 tcgacgagct gaacattctt catgcgacca tgctggcgat gcagcgtgcc gtcgctgggc
    24721 tgcatattgc gccggaatat gtgttgattg atggtaaccg ctgcccgaaa ttaccgatgc
    24781 ctgcgatggc tgtggtgaaa ggcgatagcc gcgtaccgga aatcagtgcc gcgtctatcc
    24841 tggcgaaagt gacgcgtgac gccgaaatgg cggcgctgga tattgttttc ccgcaatatg
    24901 gttttgccca acacaaaggg tacccaaccg cttttcatct ggaaaaactg gctgaacacg
    24961 gcgcgaccga acaccatcgg cgcagctttg ggcctgtcaa acgcgcactg ggacttgcgt
    25021 cctgattctt gtgtcgagat taagtaaacc ggaatctgaa gatgtctgaa ccacgtttcg
    25081 tacacctgcg ggtgcacagc gactactcga tgatcgatgg cctggccaaa accgcaccgt
    25141 tggtaaaaaa ggcggcggcg ttgggtatgc cagcactggc gatcaccgat ttcaccaacc
    25201 tttgtggtct ggtgaagttc tacggagcgg gacatggcgc agggattaag cctatcgtcg
    25261 gggcagattt taacgtccag tgcgacctgc tgggtgatga gttaacccac ctgacggtac
    25321 tggcggcgaa caataccggc tatcagaatc tgacgttgct gatctcaaaa gcgtatcagc
    25381 gcgggtacgg tgccgccggg ccgatcatcg atcgcgactg gcttatcgaa ttaaacgaag
    25441 ggttgatcct tctttccggc ggacgcatgg gcgacgtcgg acgcagtctt ttgcgtggta
    25501 acagcgcgct ggtagatgag tgtgtcgcgt tttatgaaga acacttcccg gatcgctatt
    25561 ttctcgagct gatccgcacc ggcaggccgg acgaagaaag ctatctgcac gcggcggtgg
    25621 aactggcgga agcgcgcggt ttgcccgtcg tggcgaccaa cgacgtgcgc tttatcgaca
    25681 gcagcgactt tgacgcacac gaaatccgcg tcgcgatcca cgacggcttt accctcgacg
    25741 atcctaaacg cccgcgtaac tattcgccgc agcaatatat gcgtagcgaa gaggagatgt
    25801 gcgagctgtt tgccgacatc cccgaagctc ttgccaacac cgttgagatc gccaaacgtt
    25861 gtaacgtaac cgtgcgtctt ggtgaatact tcctgccgca gttcccgacc ggggacatga
    25921 gcaccgaaga ttatctggtc aagcgtgcaa aagagggcct ggaagagcgt ctggcctttt
    25981 tattccctga cgaggaagaa cgtgttaagc gccgcccgga atatgacgaa cgtctggaga
    26041 ctgaacttca ggttatcaac cagatgggct tcccgggcta cttcctcatc gttatggaat
    26101 ttatccagtg gtcgaaagat aacggcgtac cggtagggcc aggccgtggc tccggtgcgg
    26161 gttcactggt ggcctacgcg ctgaaaatca ccgacctcga tccgctggaa tttgacctgc
    26221 tgttcgaacg tttccttaac ccggaacgtg tctccatgcc tgacttcgac gttgacttct
    26281 gtatggagaa acgcgatcag gttatcgagc acgtagcgga catgtacggt cgtgatgcgg
    26341 tatcgcagat catcaccttc ggtacaatgg cggcgaaagc ggtgatccgc gacgtaggcc
    26401 gcgtgctggg gcatccgtac ggctttgtcg atcgtatctc gaaactgatc ccgcccgatc
    26461 cggggatgac gctggcgaaa gcgtttgaag ccgagccgca gctgccggaa atctacgaag
    26521 cggatgaaga agttaaggcg ctgatcgaca tggcgcgcaa actggaaggg gtcacccgta
    26581 acgccggtaa gcacgccggt ggggtggtta tcgcgccgac caaaattacc gattttgcgc
    26641 cgctttactg cgatgaagag ggcaaacatc cggtcaccca gtttgataaa agcgacgttg
    26701 aatacgccgg actggtgaag ttcgacttcc ttggtttgcg tacgctcacc atcatcaact
    26761 gggcgctgga gatgatcaac aagcggcggg cgaagaatgg cgagccgccg ctggatatcg
    26821 ctgcgatccc gctggatgat aagaaaagct tcgacatgct gcaacgctcg gaaaccacgg
    26881 cggtattcca gcttgaatcg cgcggcatga aggacctgat caagcgtctg caacctgact
    26941 gcttcgaaga tatgatcgca ctggtggcac tgttccgccc cggcccgttg cagtcaggga
    27001 tggtggataa ctttatcgac cgtaaacatg gtcgcgaaga gatttcctat ccggacgtac
    27061 agtggcagca tgaaagcctg aaaccggtac tggagccaac ctacggcatc atcctgtatc
    27121 aggaacaggt catgcagatt gcgcaggtgc tttctggtta taccctcggt ggcgcggata
    27181 tgctgcgtcg tgcgatgggt aagaaaaagc cggaagagat ggctaagcag cgttctgtat
    27241 ttgctgaagg tgcagaaaag aacggaatca acgccgaact ggcgatgaaa atcttcgacc
    27301 tggtggagaa attcgcgggt tacggattta acaaatcgca ctctgcggcc tatgctttgg
    27361 tgtcatatca aacgttatgg ctgaaagcgc actatccggc ggagtttatg gcggcggtaa
    27421 tgaccgccga tatggacaac accgagaagg tggtgggcct ggtggatgag tgctggcgga
    27481 tggggctgaa aatcctgcca ccagatataa actccggtct ttaccatttc cacgtcaacg
    27541 acgacggcga aatcgtgtat ggtattggcg ccatcaaagg ggtaggtgaa ggtccgattg
    27601 aggccatcat cgaagcccgt aataaaggcg gctacttccg cgaactgttt gatctctgcg
    27661 cccgtaccga caccaaaaag ttaaaccggc gggtgctgga aaaactgatc atgtccgggg
    27721 cgtttgaccg tcttgggcca caccgcgcgg cgctgatgaa ctcgctgggt gatgcgttaa
    27781 aagcggcaga tcaacacgcg aaagcggaag ctatcggtca ggccgatatg ttcggcgtgc
    27841 tggccgaaga gccggaacaa attgaacaat cctacgccag ctgccaaccg tggccggagc
    27901 aggtggtgtt agatggggaa cgtgaaacgt taggtctgta ccttacggga caccctatca
    27961 accagtattt aaaagagatt gagcgttatg tcggaggcgt aaggctgaaa gacatgcacc
    28021 cgacagaacg tggtaaagtc atcacggctg cggggctcgt tgttgccgcg cgggttatgg
    28081 tcaccaagcg cggcaatcgt atcggtatct gcacgctgga tgaccgttcc gggcggctgg
    28141 aagtgatgtt gtttactgac gccctggata aataccagca attgctggaa aaagaccgca
    28201 tacttatcgt cagcggacag gtcagctttg atgacttcag cggtgggctt aaaatgaccg
    28261 ctcgcgaagt gatggatatt gacgaagccc gggaaaaata tgctcgcggg cttgctatct
    28321 cgctgacgga caggcaaatt gatgaccagc ttttaaaccg actccgtcag tctctggaag
    28381 cccaccgctc tgggacaatt ccagtacatc tctactatca gagggcggat gcacgcgcgc
    28441 ggttgcgttt tggcgcgacg tggcgtgtct ctccgagcga tcgtttatta aacgatctcc
    28501 gtggcctcat tggttcggag caggtggaac tggagtttga ctaatacagg aatactatga
    28561 gtctgaattt ccttgatttt gaacagccga ttgcagagct ggaagcgaaa atcgattctc
    28621 tgactgcggt tagccgtcag gatgagaaac tggatattaa catcgatgaa gaagtgcatc
    28681 gtctgcgtga aaaaagcgta gaactgacac gtaaaatctt cgccgatctc ggtgcatggc
    28741 agattgcgca actggcacgc catccacagc gtccttatac cctggattac gttcgcctgg
    28801 catttgatga atttgacgaa ctggctggcg accgcgcgta tgcagacgat aaagctatcg
    28861 tcggtggtat cgcccgtctt gacggtcgtc cggtgatgat cattggtcat caaaaaggtc
    28921 gtgaaaccaa agaaaaaatt cgccgtaact ttggtatgcc agcgccagaa ggttaccgca
    28981 aagcactgcg tctgatgcaa atggctgaac gctttaagat gcctatcatc acctttatcg
    29041 acaccccggg ggcttatcct ggcgtgggcg cagaagagcg tggtcagtct gaagccattg
    29101 cacgcaacct gcgtgaaatg tctcgcctcg gcgtaccggt agtttgtacg gttatcggtg
    29161 aaggtggttc tggcggtgcg ctggcgattg gcgtgggcga taaagtgaat atgctgcaat
    29221 acagcaccta ttccgttatc tcgccggaag gttgtgcgtc cattctgtgg aagagcgccg
    29281 acaaagcgcc gctggcggct gaagcgatgg gtatcatcgc tccgcgtctg aaagagctga
    29341 aactgatcga ctccatcatc ccggaaccgc tgggcggcgc tcaccgtaac ccggaagcga
    29401 tggcggtatc gttgaaagcg caactgctgg cggatctggc cgatctcgat gtgttaagca
    29461 ctgaagattt aaaaaatcgt cgttatcagc gcctgatgag ctacggttac gcgtaattcg
    29521 caaaagttct gaaaaagggt cacttcgg
//
LOCUS       DAEEKQ010000032        29064 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.032, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000032 DAEEKQ010000000
VERSION     DAEEKQ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29064)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29064)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29064
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.032"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(92..1375)
                     /gene="gltA"
                     /locus_tag="KLK57_001432"
     CDS             complement(92..1375)
                     /gene="gltA"
                     /locus_tag="KLK57_001432"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HBE0891841.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1581..1688)
                     /locus_tag="KLK57_001433"
     CDS             complement(1581..1688)
                     /locus_tag="KLK57_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891842.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2069..2473
                     /gene="sdhC"
                     /locus_tag="KLK57_001434"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2069..2473
                     /gene="sdhC"
                     /locus_tag="KLK57_001434"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBE0891843.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2467..2814
                     /gene="sdhD"
                     /locus_tag="KLK57_001435"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2467..2814
                     /gene="sdhD"
                     /locus_tag="KLK57_001435"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HBE0891844.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2814..4580
                     /gene="sdhA"
                     /locus_tag="KLK57_001436"
     CDS             2814..4580
                     /gene="sdhA"
                     /locus_tag="KLK57_001436"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HBE0891845.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLHRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4596..5312
                     /gene="sdhB"
                     /locus_tag="KLK57_001437"
     CDS             4596..5312
                     /gene="sdhB"
                     /locus_tag="KLK57_001437"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HBE0891846.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYPLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5613..8414
                     /gene="sucA"
                     /locus_tag="KLK57_001438"
     CDS             5613..8414
                     /gene="sucA"
                     /locus_tag="KLK57_001438"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HBE0891847.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKENMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRTASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8429..9646
                     /gene="odhB"
                     /locus_tag="KLK57_001439"
     CDS             8429..9646
                     /gene="odhB"
                     /locus_tag="KLK57_001439"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HBE0891848.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEDPTRLLLDV"
     gene            9740..10906
                     /gene="sucC"
                     /locus_tag="KLK57_001440"
     CDS             9740..10906
                     /gene="sucC"
                     /locus_tag="KLK57_001440"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HBE0891849.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10906..11775
                     /gene="sucD"
                     /locus_tag="KLK57_001441"
     CDS             10906..11775
                     /gene="sucD"
                     /locus_tag="KLK57_001441"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HBE0891850.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11879..12601)
                     /gene="mngR"
                     /locus_tag="KLK57_001442"
     CDS             complement(11879..12601)
                     /gene="mngR"
                     /locus_tag="KLK57_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HBE0891851.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEILIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12770..14686
                     /gene="mngA"
                     /locus_tag="KLK57_001443"
     CDS             12770..14686
                     /gene="mngA"
                     /locus_tag="KLK57_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HBE0891852.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTTYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14704..17260
                     /locus_tag="KLK57_001444"
                     /pseudo
     CDS             14704..17260
                     /locus_tag="KLK57_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            17285..17512
                     /locus_tag="KLK57_001445"
     CDS             17285..17512
                     /locus_tag="KLK57_001445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891853.1"
                     /translation="MRGQIVDKGRFVHAGYGMNALFGLQKQANSIYCRDDVDTGKRSA
                     SGNFAFIFTLKPRICGFIFNKIITLGELIII"
     gene            18108..19676
                     /gene="cydA"
                     /locus_tag="KLK57_001446"
     CDS             18108..19676
                     /gene="cydA"
                     /locus_tag="KLK57_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HBE0891854.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            19692..20831
                     /gene="cydB"
                     /locus_tag="KLK57_001447"
     CDS             19692..20831
                     /gene="cydB"
                     /locus_tag="KLK57_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBE0891855.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            20846..20959
                     /gene="cydX"
                     /locus_tag="KLK57_001448"
     CDS             20846..20959
                     /gene="cydX"
                     /locus_tag="KLK57_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HBE0891856.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            20959..21252
                     /gene="ybgE"
                     /locus_tag="KLK57_001449"
     CDS             20959..21252
                     /gene="ybgE"
                     /locus_tag="KLK57_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HBE0891857.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            21402..21806
                     /gene="ybgC"
                     /locus_tag="KLK57_001450"
     CDS             21402..21806
                     /gene="ybgC"
                     /locus_tag="KLK57_001450"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HBE0891858.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPCALPKSIVAEFKQ"
     gene            21803..22495
                     /gene="tolQ"
                     /locus_tag="KLK57_001451"
     CDS             21803..22495
                     /gene="tolQ"
                     /locus_tag="KLK57_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HBE0891859.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            22499..22927
                     /gene="tolR"
                     /locus_tag="KLK57_001452"
     CDS             22499..22927
                     /gene="tolR"
                     /locus_tag="KLK57_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HBE0891860.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            22992..24224
                     /gene="tolA"
                     /locus_tag="KLK57_001453"
     CDS             22992..24224
                     /gene="tolA"
                     /locus_tag="KLK57_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HBE0891861.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALLWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKIKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAAAEKAAA
                     DKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEKAAADKKAAAAKAAAEKAAA
                     AKAAAEADDIFGELSSGKNAPKTGGGAKGNNASSAGSGNTKNNGASGADINNYAGQIK
                     SAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKIPKP
                     PSQAVYEVFKNAPLDFKP"
     gene            24357..25649
                     /gene="tolB"
                     /locus_tag="KLK57_001454"
     CDS             24357..25649
                     /gene="tolB"
                     /locus_tag="KLK57_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HBE0891862.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            25684..26205
                     /gene="pal"
                     /locus_tag="KLK57_001455"
     CDS             25684..26205
                     /gene="pal"
                     /locus_tag="KLK57_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HBE0891863.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            26215..27006
                     /gene="cpoB"
                     /locus_tag="KLK57_001456"
     CDS             26215..27006
                     /gene="cpoB"
                     /locus_tag="KLK57_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HBE0891864.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            27171..27246
                     /locus_tag="KLK57_001457"
     tRNA            27171..27246
                     /locus_tag="KLK57_001457"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27204..27206,aa:Lys,seq:ttt)
     gene            27396..27471
                     /locus_tag="KLK57_001458"
     tRNA            27396..27471
                     /locus_tag="KLK57_001458"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27429..27431,aa:Val,seq:tac)
     gene            27475..27550
                     /locus_tag="KLK57_001459"
     tRNA            27475..27550
                     /locus_tag="KLK57_001459"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27508..27510,aa:Lys,seq:ttt)
     gene            27599..27674
                     /locus_tag="KLK57_001460"
     tRNA            27599..27674
                     /locus_tag="KLK57_001460"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27632..27634,aa:Lys,seq:ttt)
     gene            27708..27783
                     /locus_tag="KLK57_001461"
     tRNA            27708..27783
                     /locus_tag="KLK57_001461"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:27741..27743,aa:Lys,seq:ttt)
     gene            27808..27939
                     /locus_tag="KLK57_001462"
     ncRNA           27808..27939
                     /ncRNA_class="other"
                     /locus_tag="KLK57_001462"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            28188..>29064
                     /gene="nadA"
                     /locus_tag="KLK57_001463"
     CDS             28188..>29064
                     /gene="nadA"
                     /locus_tag="KLK57_001463"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBE0891865.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMLTLQAECSLDLGCPVEEFNAFCDAHPDHTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSFGEKIIWAPDKHLGRYVQKQTGADILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCR"
BASE COUNT         7108 a         7099 c         7825 g         7032 t
ORIGIN      
        1 ttatttttac aattttttgt gtttgggcat ataaaaatta acccgccatt tgaacggcgg
       61 gttaaaatat ttacaactta gcaatcaacc attaacgctt gatatcgctt ttaaagtcgc
      121 gtttttcata tcctgtatac agctgacgcg gacgggcaat cttcataccg tcactgtgca
      181 tttcgctcca gtgggcgatc cagccaacgg tacgtgccat tgcgaaaatg acggtgaaca
      241 tggaagacgg aatacccatc gctttcagga tgataccaga gtagaaatcg acgttcgggt
      301 acagtttctt ctcgataaag tacgggtcgt tcagcgcgat gttttccagc tccatagcca
      361 cttccagcag gtcatctttg gtgcccagct ctttcagaac ttcatggcag gtttcacgca
      421 ttacggtggc gcgcgggtcg taatttttgt acacgcggtg accgaagccc atcaggcgga
      481 aagaatcatt tttatctttc gcacgacgaa caaattccgg aatgtgttta acggagctaa
      541 tttcttccag cattttcagc gccgcttcgt tagcaccacc gtgcgcaggt ccccacagtg
      601 aagcaatacc tgctgcgata caggcaaacg ggttcgcacc cgaagagcca gcggtacgca
      661 cggtggaggt agaggcgttc tgttcatggt cagcgtgcag gatcagaata cggtccatag
      721 cacgttccag aatcggatta acttcatacg gttcgcacgg cgtggagaac atcatattca
      781 ggaagttacc ggcataggag agatcgttgc gcggataaac aaatggctga ccaatggaat
      841 acttgtaaca catcgcggcc atggtcggca ttttcgacag caggcggaac gcggcaattt
      901 cacggtgacg aggattgtta acatccagcg agtcgtgata gaacgccgcc agcgcgccgg
      961 taataccaca catgactgcc attggatgcg agtcgcgacg gaaagcatgg aacagacggg
     1021 taatctgctc gtggatcatg gtatgacggg tcaccgtagt tttaaattcg tcatactgtt
     1081 cctgagtcgg tttttcacca ttcagcagga tgtaacaaac ttccaggtag ttagaatcgg
     1141 tcgccagctg atcgatcggg aaaccgcggt gcagcaaaat accttcatca ccatcaataa
     1201 aagtaatttt agattcgcag gatgcggttg aagtgaagcc tgggtcaaag gtgaacacac
     1261 cttttgaacc gagagtacgg atatcaataa catcttgacc cagcgtgcct ttcagcacat
     1321 ccagttcaac agctgtgtcc ccgttgaggg tgagttttgc ttttgtatca gccatttaag
     1381 gtctccttag cgccttattg cgtaagactg ccggaactta aatttgcctt cgcacatcaa
     1441 cctggcttta cccgtttgtt atttggctcg ccgctctgtg aaagagggga aaacctgggt
     1501 acagagctct gggcgcttgc aggtaaagga tccattgatg acgaataaat ggcgaatcaa
     1561 gtacttagca atccgaatta ttaaacttgt ctaccactaa taactgtccc gaatgaattg
     1621 gtcaatactt ccacactgtt acataagtta atcttaggtg aaataccgac ttcataactt
     1681 ttacgcatta tatgcttttc ctggcaatgt ttgtaacaac tttgttgaat gattgtcaaa
     1741 ttagatgatt aaaaattaaa taaatgttgt tatcgtgacc tggatcactg ttcaggataa
     1801 aacccgacaa actatatgta ggttaattgt aatgattttg tgaacagcct atactgccgc
     1861 caggtctccg gaacaccctg caatcccgag ccacccagcg ttgtaacgtg tcgttttcgc
     1921 atctggaagc agtgttttgc atgacgcgca gttatagaaa ggacgctgtc tgacccgcaa
     1981 gcagaccgga ggaaggaaat cccgacgtct ccaggtaaca gaaagttaac ctctgtgccc
     2041 gtagtcccca gggaataata agaacagcat gtgggcgtta ttcatgataa gaaatgtgaa
     2101 aaaacaaaga cctgttaatc tggacctaca gaccatccgg ttccccatca cggcgatagc
     2161 gtccattctc catcgcgttt ccggtgtgat cacctttgtt gcagtgggca tcctgctgtg
     2221 gcttctgggt accagcctct cttcccctga aggtttcgag caagcttccg cgattatggg
     2281 cagcttcttc gtcaaattta tcatgtgggg catccttacc gctctggcgt atcacgtcgt
     2341 cgtaggtatt cgccacatga tgatggattt tggctatctg gaagaaacat tcgaagcggg
     2401 taaacgctcc gccaaaatct cctttgttat tactgtcgtg ctttcacttc tcgcaggagt
     2461 cctcgtatgg taagcaacgc ctccgcatta ggacgcaatg gcgtacatga tttcatcctc
     2521 gttcgcgcta ccgctatcgt cctgacgctc tacatcattt atatggtcgg ttttttcgcc
     2581 accagtggcg aactgacata tgaagtctgg atcggtttct tcgcctctgc gttcaccaaa
     2641 gtgttcaccc tgctggcgct gttttctatc ttgattcatg cctggatcgg catgtggcag
     2701 gtgttgaccg actacgttaa accgctggca ttgcgcctga tgctgcaact ggtgattgtc
     2761 gttgcactgg tggtttacgt gatttatgga ttcgttgtgg tgtggggtgt gtgatgaaat
     2821 tgccagtcag agaatttgat gcagttgtga ttggtgccgg tggcgcaggt atgcgcgcgg
     2881 cgctgcaaat ttcccagagc ggccagacct gtgcgctgct ctctaaagtc ttcccgaccc
     2941 gttcccatac cgtttctgcg caaggcggca ttaccgttgc gctgggtaat acccatgaag
     3001 ataactggga atggcatatg tacgacaccg taaaagggtc ggactatatc ggtgaccagg
     3061 acgcgattga atatatgtgt aaaaccgggc cggaagcgat tctggaactc gaacacatgg
     3121 gcctgccgtt ctcgcgtctt gatgatggtc gtatctatca acgtccgttt ggcggtcagt
     3181 cgaaaaactt cggcggcgag caggcggcac gcactgcggc ggcggctgac cgtaccggtc
     3241 acgcactgtt gcacacgctt tatcagcaga acctgaaaaa ccacaccacc attttctccg
     3301 agtggtatgc gctggatctg gtgaaaaacc aggatggcgc ggtggtgggt tgtaccgcac
     3361 tgtgcatcga aaccggtgaa gtggtttatt tcaaagcccg cgctaccgtg ctggcgactg
     3421 gcggggcagg gcgtatttat cagtccacca ctaacgccca cattaacact ggcgacggtg
     3481 tcggcatggc tatccgcgca ggcgtaccgg tgcaggatat ggaaatgtgg caattccacc
     3541 cgaccggcat tgccggtgcg ggcgtactgg tcaccgaagg ttgccgtggt gaaggcggtt
     3601 atctgctgaa caaacatggc gaacgcttta tggagcgtta tgcgccgaac gccaaagacc
     3661 tggcgggccg tgatgtggtg gcgcgttcca tcatgattga aatccgtgaa ggtcgcggct
     3721 gtgatggtcc gtgggggcca cacgcgaaac tgaaactcga tcacctgggt aaagaagttc
     3781 tcgaatcccg tctgccgggt atcctggagc tttcccgtac ctttgctcac gtcgatccgg
     3841 tgaaagagcc gattccggtt atcccaacct gtcactacat gatgggcggt attccgacca
     3901 aagtgaccgg tcaggcgctg acggtgaacg agaaaggcga agatgtggtt gttccggggc
     3961 tgtttgccgt tggtgaaatc gcttgtgtat cggtacacgg cgctaaccgt ctgggcggca
     4021 actcgctgct ggacctggtg gtctttggtc gtgcggcagg tctgcatctg caagagtcta
     4081 tcgccgaaca gggcgcattg cgcgatgcca gcgagtctga tgttgaagca tctctggatc
     4141 gtctgcaccg ctggaacaat aatcgtaacg gtgaagatcc ggtggcgatt cgtaaagcgc
     4201 tgcaagaatg tatgcagcat aacttctcgg tcttccgtga aggtgatgcg atggcgaaag
     4261 ggcttgagca gttgaaagtg atccgcgagc gtctgaaaaa tgcccgtctg gatgacactt
     4321 ccagcgagtt caacacccag cgcgttgagt gcttggaact ggataacctg atggaaacgg
     4381 cgtatgcaac ggctgtttct gccaacttcc gtaccgaaag ccgtggcgca catagccgct
     4441 tcgacttccc ggatcgtgat gatgaaaact ggctgtgcca ctccctgtat ctgccagagt
     4501 cggaatccat gacgcgccga agcgtcaaca tggaaccgaa actgcgcccg gcattcccgc
     4561 cgaagattcg tacttactaa tgcggagaca ggaaaatgag actcgagttt tcaatttatc
     4621 gctataaccc ggatgttgat gatgctccgc gtatgcagga ttaccccctg gaagcggaag
     4681 aaggtcgcga catgatgctg ctggatgcgc ttatccagct gaaagagaaa gatcccagcc
     4741 tgtcgttccg ccgctcctgc cgtgaaggtg tgtgcggttc cgacggtctg aacatgaacg
     4801 gcaagaatgg tctggcctgt attaccccga tttcggcact caaccagccg ggcaagaaga
     4861 ttgtgattcg cccgctgcca ggtttaccgg tgatccgcga tttggtggta gacatgggac
     4921 aattctatgc gcaatatgag aaaattaagc cttacctgtt gaataatgga caaaatccgc
     4981 cagctcgcga gcatttacag atgccagagc agcgcgaaaa actcgacggg ctgtatgaat
     5041 gtattctctg cgcatgttgt tcaacctctt gtccgtcttt ctggtggaat cccgataagt
     5101 ttatcggccc ggcaggcttg ttagcggcat atcgtttcct gatcgatagc cgtgataccg
     5161 agactgacag ccgcctcgac ggtttgagcg atgcattcag cgtattccgc tgtcacagca
     5221 tcatgaactg cgtcagtgta tgtccgaagg ggctgaaccc gacgcgcgcc atcggccata
     5281 ttaagtcgat gttgttgcaa cgtaatgcgt aaaccgtagg cctgataaga cgcgcaagcg
     5341 tcgcatcagg caaccagtgc cggatgcggc gtgaacgcct tatccggcct acaagtcatt
     5401 acccgtaggc ctgataagcg cagcgcatca ggcgtaacaa agaaatgcag gaaatcttta
     5461 aaaactgccc ctgacactaa gacagttttt aaaggttcct tcgcgagcca ctacatagac
     5521 aagagctcgc aagtgaaccc cggcacgcac atcactgtgc gtggtagtat ccacggcgaa
     5581 gtaagcataa aaaagatgct taagggatca cgatgcagaa cagcgctttg aaagcctggt
     5641 tggactcttc ttacctctct ggcgcaaacc agagctggat agaacagctc tatgaagact
     5701 tcttaaccga tcctgactcg gttgacgcta actggcgttc gacgttccag cagttacctg
     5761 gtacgggagt caaaccggat caattccact ctcaaacgcg tgaatatttc cgccgcctgg
     5821 cgaaagacgc ttcacgttac tcttcaacga tctccgaccc tgacaccaat gtgaagcagg
     5881 ttaaagtcct gcagctcatt aacgcatacc gcttccgtgg tcaccagcat gcgaatctcg
     5941 atccgctggg actgtggcag caagataaag tggccgatct ggatccgtct ttccacgatc
     6001 tgaccgaagc agacttccag gagaccttca acgtcggttc atttgccagc ggcaaagaaa
     6061 acatgaaact cggcgagctg ctggaagccc tcaagcaaac ctactgtggc ccgattggtg
     6121 ccgagtatat gcacattacc agcaccgaag aaaaacgctg gatccaacag cgtatcgagt
     6181 ctggtcgcgc gactttcaat agcgaagaga aaaaacgctt cttaagcgaa ctgaccgccg
     6241 ctgaaggcct tgaacgttac ctcggcgcaa aattccctgg cgcaaaacgc ttctcgctgg
     6301 aaggcggtga cgcgttaatc ccgatgctta aagagatgat ccgccacgct ggcaacagcg
     6361 gcacccgcga agtggttctt gggatggcgc accgtggtcg tctgaacgtg ctggtgaacg
     6421 tgctgggtaa aaaaccgcaa gacttgttcg acgagtttgc cggtaaacat aaagaacacc
     6481 tcggcacggg tgacgtgaaa taccacatgg gcttctcgtc tgacttccag accgatggcg
     6541 gcctggtgca cctggcgctg gcgtttaacc cgtctcacct tgagattgta agcccggtag
     6601 ttatcggttc tgttcgtgcc cgtctggaca gacttgatga gccgagcagc aacaaagtgc
     6661 tgccaatcac catccacggt gacgccgcag tgaccgggca gggcgtggtt caggaaactc
     6721 tgaacatgtc gaaagcgcgt ggttatgaag ttggcggtac ggtacgtatc gttatcaaca
     6781 accaggttgg tttcaccacc tctaatccac tggatgctcg ttctacaccg tactgtactg
     6841 atatcggtaa gatggttcag gcaccgattt tccacgttaa cgcggatgat ccggaagccg
     6901 ttgcctttgt gacccgtctg gcgctcgatt tccgtaacac ctttaaacgt gatgtcttca
     6961 tcgacctggt gtgctaccgc cgtcacggcc acaacgaagc cgacgagccg agcgcaaccc
     7021 agccgctgat gtatcagaaa atcaaaaaac atccgacacc gcgcaaaatc tacgctgaca
     7081 agctggagca ggaaaaagtg gcgacgctgg aagatgccac cgagatggtt aacctgtacc
     7141 gcgatgcgct ggatgctggc gattgcgtag tggcagagtg gcgtccgatg aacatgcact
     7201 ctttcacctg gtcgccgtac ctcaaccacg aatgggacga agagtacccg aacaaagttg
     7261 agatgaagcg cctgcaggag ctggcgaaac gcatcagcac ggtgccggaa gcagttgaaa
     7321 tgcagtctcg cgttgccaag atttatggcg atcgccaggc gatggctgcc ggtgagaaac
     7381 tgttcgactg gggcggtgcg gaaaacctcg cttacgccac gctggttgac gaaggcattc
     7441 cggttcgcct gtcgggtgaa gactccggtc gcggtacctt cttccaccgc cacgcggtga
     7501 tccacaacca gtctaacggt tccacttaca cgccgttgca acacatccat aacgggcagg
     7561 gcgcgttccg cgtctgggac tccgtactgt ctgaagaagc agtgctggcg tttgaatacg
     7621 gttatgccac cgcagaacca cgcactctga ccatctggga agcgcagttc ggtgacttcg
     7681 ccaacggtgc gcaggtggtt atcgaccagt tcatctcttc tggcgaacag aaatggggcc
     7741 ggatgtgtgg tctggtgatg ttgctgccgc acggttacga agggcagggg ccggagcact
     7801 cctccgcgcg tctggaacgt tatctgcaac tttgtgctga gcaaaacatg caggtttgcg
     7861 ttccgtctac cccggcacag gtttaccaca tgctgcgtcg tcaggcgctg cgcgggatgc
     7921 gtcgtccgct ggtagtgatg tcgccgaaat ccctgctgcg tcatccgctg gcggtttcca
     7981 gcctcgaaga actggcgaac ggcaccttcc tgccagccat cggtgaaatc gacgagcttg
     8041 atccgaaggg cgtgaagcgc gtagtgatgt gttctggtaa ggtttattac gacctgctgg
     8101 aacagcgtcg taagaacaat caacacgatg tcgccattgt gcgtatcgag caactctacc
     8161 cgttcccgca taaagcgatg caggaagtgt tgcagcagtt tgctcacgtc aaggattttg
     8221 tctggtgcca ggaagagccg ctcaaccagg gcgcatggta ctgcagccag catcatttcc
     8281 gtgaagtgat tccgtttggg gcttctctgc gttatgcagg ccgcacggcc tccgcctctc
     8341 cggcggtagg gtatatgtcc gttcaccaga aacagcaaca agatctggtt aatgacgcgc
     8401 tgaacgtcga ataaataaag gatacacaat gagtagcgta gatattctgg tccctgacct
     8461 gcctgaatcc gtagccgatg ccaccgtcgc aacctggcat aaaaaacccg gcgacgcagt
     8521 cgtacgtgat gaagtgctgg tagaaatcga aactgacaaa gtggtactgg aagtaccggc
     8581 atcagcagac ggcattctgg atgcggttct ggaagatgaa ggtacaacgg taacgtctcg
     8641 tcagatcctt ggtcgcctgc gtgaaggcaa cagcgccggt aaagaaacca gcgccaaatc
     8701 tgaagagaaa gcgtccactc cggcgcaacg ccagcaggcg tctctggaag agcaaaacaa
     8761 cgatgcgtta agcccggcga tccgtcgcct gctggctgaa cacaatctcg acgccagcgc
     8821 cattaaaggc accggtgtgg gtggtcgtct gactcgtgaa gatgtggaaa aacatctggc
     8881 gaaagccccg gcgaaagagt ctgctccggc agcggctgct ccggcggcgc aaccggctct
     8941 ggctgcacgt agtgaaaaac gtgtcccgat gactcgcctg cgtaagcgtg tggcagagcg
     9001 tctgctggaa gcgaaaaact ccaccgccat gctgaccacg ttcaacgaag tcaacatgaa
     9061 gccgattatg gatctgcgta agcagtacgg tgaagcgttt gaaaaacgcc acggcatccg
     9121 tctgggcttt atgtccttct acgtgaaagc ggtggttgaa gccctgaaac gttacccgga
     9181 agtgaatgct tctatcgacg gtgatgacgt ggtttaccac aactatttcg acgtcagcat
     9241 ggcggtttcc acgccgcgcg gcctggtgac gccggtactg cgtgatgtcg ataccctcgg
     9301 catggcagac atcgagaaga aaatcaaaga gctggcagtt aaaggccgtg acggcaagct
     9361 gacggttgaa gatctgaccg gtggtaactt caccatcacc aacggtggtg tgttcggttc
     9421 cctgatgtct acgccgatca tcaacccgcc gcagagcgca attctgggta tgcacgctat
     9481 caaagatcgt ccgatggcgg tgaatggtca ggttgagatc ctgccgatga tgtacctggc
     9541 gctgtcctac gatcaccgtc tgatcgatgg tcgcgaatcc gtgggcttcc tggtaacgat
     9601 caaagagttg ctggaagatc cgacgcgtct gctgctggac gtgtagtagt ttaagcttca
     9661 cctgcactgt agaccgacgt ggatagggtg tcggtctacg gtttaaaaga taacgattac
     9721 tgaaggatgg acagaacaca tgaacttaca tgaatatcag gcaaaacaac tttttgcccg
     9781 ctatggctta ccagcaccgg tgggttatgc ctgtactact ccgcgcgaag cagaagaagc
     9841 cgcttcaaaa atcggtgccg gcccgtgggt agtgaaatgt caggttcacg ctggtggccg
     9901 cggtaaagcg ggcggtgtga aagttgtaaa cagcaaagaa gacatccgtg cttttgcaga
     9961 aaactggctc ggcaagcgtc tggtaacgta tcaaacagat gccaatggcc aaccggttaa
    10021 ccagattctg gttgaagcag cgaccgatat cgctaaagag ctgtatctcg gtgccgttgt
    10081 agaccgtagt tcccgtcgtg tggtctttat ggcctccacc gaaggcggcg tggaaatcga
    10141 aaaagtggcg gaagaaactc cgcacctgat ccacaaagtt gcgcttgatc cgctgactgg
    10201 cccgatgccg tatcagggac gcgagctggc gttcaaactg ggtctggaag gtaaactggt
    10261 tcagcagttc accaaaatct tcatgggcct ggcgaccatt ttcctggagc gcgacctggc
    10321 gttgatcgaa atcaacccgc tggtcatcac caaacagggc gatctgattt gcctcgacgg
    10381 caaactgggc gctgacggca acgcactgtt ccgccagcct gatctgcgcg aaatgcgtga
    10441 ccagtcgcag gaagatccgc gtgaagcaca ggctgcacag tgggaactga actacgttgc
    10501 gctggacggt aacatcggtt gtatggttaa cggcgcaggt ctggcgatgg gtacgatgga
    10561 catcgttaaa ctgcacggcg gcgaaccggc taacttcctt gacgttggcg gcggcgcaac
    10621 caaagaacgt gtaaccgaag cgttcaaaat catcctctct gacgacaaag tgaaagccgt
    10681 tctggttaac atcttcggcg gtatcgttcg ttgcgacctg atcgctgacg gtatcatcgg
    10741 cgcggtagca gaagtgggtg ttaacgtacc ggtcgtggta cgtctggaag gtaacaacgc
    10801 cgaactcggc gcgaagaaac tggctgacag cggcctgaat attattgcag caaaaggtct
    10861 gacggatgca gctcagcagg ttgttgccgc agtggagggg aaataatgtc cattttaatt
    10921 gataaaaaca ccaaggttat ctgccagggc tttactggta gccaggggac tttccactca
    10981 gaacaggcca ttgcatacgg cactaaaatg gttggcggcg taaccccagg taaaggcggt
    11041 actacccacc tcggcctgcc ggtgttcaac accgtgcgtg aagctgttgc tgctactggc
    11101 gctaccgctt ctgttatcta cgtaccagca ccgttctgca aagactccat tctggaagct
    11161 attgatgcag gcatcaaact gattatcacc atcactgaag gcatcccgac gctggatatg
    11221 ctgactgtga aagtgaaact ggacgaagcc ggcgttcgta tgatcggccc gaactgcccg
    11281 ggcgttatca ctccgggtga atgcaaaatc ggtatccagc cgggtcacat tcacaaaccg
    11341 ggtaaagtgg gtatcgtttc ccgttccggt acactgacct atgaagcggt taaacagacc
    11401 acggattacg gtttcggtca gtcgacctgt gttggtatcg gcggtgaccc gatcccaggc
    11461 tctaacttta tcgacatcct cgaaatgttc gaaaaagatc cgcagaccga agcgatcgtg
    11521 atgatcggtg agatcggcgg tagcgctgaa gaagaagcgg cagcgtacat caaagagcac
    11581 gttaccaagc cagttgtagg ttacatcgct ggtgtgactg cgccgaaagg caaacgtatg
    11641 ggccacgcgg gtgcgatcat tgctggtggg aaagggactg cggatgagaa attcgctgct
    11701 ctggaagccg caggcgtgaa aaccgttcgc agcctggcgg atatcggtga agcactgaaa
    11761 actgttctga aataaatatc tgtaataaga aatagccctc gccgcttccc tctacaggaa
    11821 tggcgaaggg ctgtcggttt cgacatggtt ggccatcgta tgatggcctt ttttgtgctt
    11881 atcgcgatga ttttcgctgc gctatcaggg taaatttata gtcatcggta ttaaaagcgt
    11941 tgcggctata ttcaaacacc cgaccatcaa ctaaatatcc acgcgatact ttttcaagaa
    12001 tcggctttgt ctggctgata ttaagcagac ggctcatctc ttcggttggc atcagaggaa
    12061 tgatttcctg ttcgctacga tcgataacca tcttcttcac ttcttcaata aagtgatatt
    12121 tcgaattttc catgacctgc caggtgagat ccgggaacaa cgcaagcggc atccaggttt
    12181 cttccagcgc cattggcttt tgcttgcgat agcgcacgcg cttcacatgc cacacacgat
    12241 cctgcgcggt gatttgtagc tgttgctgaa gaaaatcgtc agccggaatc acttcaaata
    12301 tcaggatttc actgtgtgta tcgacgtgac ggtccgacag tttttcatca aaactggtta
    12361 actgaaaaat atcgtaattg acccgctctt ctttgacgta agtcccgctg ccctgaatgc
    12421 tttcgaggat ctgctgctcg actagctggc gcaaagcctg acgcaccgta acccggctga
    12481 cgccaaactc tgtttgtagc gctgattcag tgggtaacgc atcgccaggt ttaagctcgc
    12541 cacgcgcaat ttgttcacga atgcgatcgg caatctgccg gtataagggc ttgtgtccca
    12601 tttttagtat ctcattaata tgaatttagc cattatgccc gataaattca tcctgtaaat
    12661 aatacaaata caatacaaat aatttcaatc aagtgaaatt gatcacatga tggtattgtt
    12721 ttatcgggca cactggcgcg actataaaaa cgatcaagtg aggatcatga tgaatctgac
    12781 gactctgacc caccgcgatg cgttgtgtct gaatgcgcgc tttaccagcc gtgaagaggc
    12841 catccacgcg ttgactcaac gtcttgctgc tctggggaaa atttccagta ctgagcaatt
    12901 tctggaagaa gtgtatcgcc gtgaaagcct tggcccgacc gccttaggtg aagggttggc
    12961 tgtgccgcat ggcaaaactg ctgcggtaaa agaagcggcg tttgcggtcg ccacactcag
    13021 cgagccgctt cagtgggaag gcgttgatgg cccggaagca gttgatttag tggtgctgct
    13081 ggcgattccc cccaatgaag cgggtactac gcatatgcaa ctgctgacag cgctgaccac
    13141 gcgccttgcg gatgatgaga ttcgggcgcg tatacagtcg gcgacgacgc ctgatgagtt
    13201 gctctcggcg ctggatgaca agggaggcac gcaaccttct gcctcttttt ccaacgcgcc
    13261 aactatcgtc tgcgtaacgg cctgtccggc gggtattgct cacacctata tggctgcgga
    13321 atatctggaa aaagccggac gcaaactcgg cgtaaatgtt tacgttgaaa aacaaggcgc
    13381 taacggcatt gaagggcgtt taacggcgga tcaactcaat agtgcaaccg cctgtatttt
    13441 tgcggctgaa gtcgccatca aggagagtga gcgttttaat ggcattcccg cgctttcagt
    13501 gcctgttgcc gagccgattc gccatgcaga agcgttgatc caacaagcgc ttaccctcaa
    13561 gcgtagcgat gagacgcgta ccgtacagca agatacgcaa ccggtgaaaa gtgtcaaaac
    13621 ggagctgaaa caggcactgt tgagcggaat ctcttttgcc gtaccgttga ttgtcgcggg
    13681 gggcacggtg ctggcggtcg cggtattact gtcgcaaatc ttcgggctac aagatctgtt
    13741 taatgaagaa aactcctggc tgtggatgta ccgcaagctg ggcggcgggc tgctcggaat
    13801 tttgatggta ccggtgctcg cggcctatac cacctattct ctggcagata aaccggcgtt
    13861 agcgccaggc tttgcggctg gacttgccgc caacatgatc ggctccgggt ttctcggcgc
    13921 ggtcgttggc ggattgatag ccggttactt gatgcgctgg gtgaaaaatc acttgcgtct
    13981 tagcagtaaa ttcaatggat tcctgacttt ttatctctac ccggtgctcg gtacgttggg
    14041 agcgggcagt ctgatgctgt ttgtggtggg agaacctgtc gcatggatca ataactcgct
    14101 taccgcctgg ctgaacggtc tgtcaggaag taacgcgctg ttgctgggtg ccattctcgg
    14161 ttttatgtgt tcctttgacc ttggagggcc agtgaacaaa gccgcttatg cattctgcct
    14221 gggcgcaatg gcgaacggcg tttacggccc gtatgccatt ttcgcctccg tcaaaatggt
    14281 ttcggcattt accgtaaccg cttccacgat gctcgcaccg cgcctgttta aagagtttga
    14341 aattgagacc gggaaatcca cctggctgtt agggctggca ggtattaccg aaggggcgat
    14401 cccgatggcg attgaagatc cgctgcgggt tattggttcg tttgtgctgg gctctatggt
    14461 aacgggcgct attgtcggtg cgatgaatat cggcctttcg acacccggtg ccggcatttt
    14521 ctcgctcttt ttacttcatg ataatggcgc gggcggtgtt atggcggcaa ttggctggtt
    14581 tggtgcggca ttggtggggg ctgcaatctc gactgcaatt ctcctgatct ggcggcgtca
    14641 cgcggttaag catggcaact atctgactga tggcgtaatg ccataaacaa aaaggaaacg
    14701 acgatgaaag cggtatctcg cgttcacatc accccgcata tgcattggga tcgggagtgg
    14761 tatttcacca ccgaagagtc acgcattctg ctggtcaata atatggaaga gatcctgtgc
    14821 cgactggaac aggacaacga atacaaatat tacgtactcg acgggcaaac ggcgatcctc
    14881 gaagattatt tcgcggtgaa accggaaaac aaagaccgcg tgaagaaaca ggtagaagcc
    14941 ggtaagttga ttatcggccc ctggtatacc cagaccgata ccacgattgt ttctgcggaa
    15001 tccatcgtcc gtaatctgat gtacggaatg cgtgactgcc tcgcgtttgg cgagccgatg
    15061 aaaataggtt atttaccgga ttcctttggc atgtccgggc aactgccgca tatttacaat
    15121 ggatttggca ttacccgcac tatgttctgg cgcggatgtt cggagcgcca cggtactgat
    15181 aaaaccgagt ttttgtggca aagcagtgac ggtagcgaag tgacggcgca ggtgctgccg
    15241 ctgggctacg ccatcggtaa gtacttacct gccgacgaaa acggattacg taaacgcctc
    15301 gacagttatt ttgacgtgct ggaaaaagcg tctgtaacca aagagatttt gctgccgaat
    15361 ggtcatgacc agatgccatt gcagcaaaat atcttcgaag tgatggataa gctacgtgag
    15421 atctaccctc aacgtaagtt tgtgatgagc cgctttgaag aggtatttga gaagatcgaa
    15481 gcgcagcgtg aaagtctggc caccctgaaa ggtgaattta tcgatggcaa atatatgcgc
    15541 gtacatcgca ccatcggttc cacgcgcatg gatatcaaaa ttgcccacgc acgtattgaa
    15601 aataagattg ttaatctgct ggaaccgctg gcaacgctgg cctggacgtt gggttttgaa
    15661 taccaccacg gcttgctgga gaaaatgtgg aaagagatct taaaaaatca tgcccacgac
    15721 agtatcggtt gttgttgcag tgacaaagtt catcgcgaaa ttgtcgcccg cttcgaactg
    15781 gctgaagaca tggcggataa tctgctgcgt ttctacatgc gcaaaattgc cgataacatg
    15841 ccacagagtg atgccgacaa actcgtgctg tttaacctga tgccctggcc gcgggaagaa
    15901 gttattaaca ccactgtgcg gctgcgcgcc agccagttta atctacaaga cgatcgcggt
    15961 cagcctgtac cgtattttat tcgccatgcc cgtgagatcg atcctggcct aatcgatcgg
    16021 caaattgttc attatggcaa ttatgatccc tttatggagt ttgatattca gatcaaccag
    16081 attgtccctt ctatgggcta tcgcacgctt tatattgaag cgaatcagcc tggcaacgtg
    16141 attgcggcaa aaagtgacgc tgaagggatt ctggaaaatg ctttctggca gattgcgctc
    16201 aatgaggatg gttctctgca actggtagat aaagacagcg gtgtgcgcta tgaccgggta
    16261 ttgcaaattg aagaaagctc tgatgatggt gatgaatatg actattcacc cgcaaaagaa
    16321 gagtgggtaa ttaccgcagc gaacgcgaaa ccgcaatgcg atattattca tgaagcctgg
    16381 cagagtaggg ggggtgttgg ttgtcactct tagtcataac agcaggcgta ttgatgtgga
    16441 tatcaacctt gataaccagg ctgacgatca tcgccttcgt gtcctggtcc ctacaccttt
    16501 taacactgac agtgttctgg cagatacgca gtttggttcg ctaacgcgcc ccgtgaacga
    16561 cagtgcaatg aacaactggc agcaagaagg ctggaaagaa gcgccggttc cggtatggaa
    16621 tatgctcaac tatgttgcct tacaggaagg gcgtaacggc atggctgtct ttagcgaagg
    16681 gttacgtgaa tttgaagtca tcggtgaaga gaagaaaacc tttgccatta cgttgctgcg
    16741 tggcgtgggc ttactgggca aagaagatct gcttttaagg cctgggcggc cttcgggaat
    16801 taaaatgcca gtcccggact cacaactacg tggtctgctt tcttgtcgcc taagtttatt
    16861 gagttatacc ggtacgccaa ccgccgctgg tgtagctcag caggcgcgag catggctgac
    16921 tccagtacag tgttacaaca aaatcccatg ggatgcgatg aagctcaaca aagccggatt
    16981 caacgtgccg gaaagttata gtttgttgaa aatgccccca gtgggatgcc tgataagcgc
    17041 acttaagaaa gctgaagacc gacaagaagt gattttacgg ctgtttaatc cggctgaatc
    17101 agcaacctgt gatgcgactg ttgctttcag tcgcgaggtg atttcttgct cagaaacgat
    17161 gatggatgaa cacattacca ccgaggaaaa tcaaggttca aatctatcgg ggcctttttt
    17221 acccggccag tcacggacgt tcagttaccg gcttgcctga atagcaatca aaccgaagcc
    17281 acatatgcgc ggccagattg ttgacaaagg gcgctttgtt catgccggat acggcatgaa
    17341 cgctttattc ggtctacaaa agcaggcaaa ttcaatatat tgcagagatg atgtagacac
    17401 tggcaagcgt agcgcatcag gtaattttgc gtttatcttc actctcaagc cacgtatatg
    17461 tggctttatt tttaacaaaa taataaccct gggtgagtta attataatat aattacaagt
    17521 taattaaatg ttaatatcgg cggggtggat ttatgccttt attagtaatc ctgaaactct
    17581 gcgtcgtatt agccagtgac caaaaaaaga attaaggtca accgtgctgt ttttgcttcg
    17641 tctcttttta tctttaaatt gccaaccgaa actaatttca gccttataac tcacacattt
    17701 taaacataaa tgtcactaaa gttaccttat tgaaacatga ttaacataat ttgtaggaat
    17761 tgatatttat caatgtataa gtcttggaaa ttggcatcaa aaagagataa attgttctcg
    17821 atcaaattgg ctgaaaggcg gtaatttagc tataaattga tcaccgtcga aaaatgcaaa
    17881 tttgcttcaa caaaaacctg tttattgtaa ggattttgca gcgtaatata tacgtgggat
    17941 caatttgagt ttttattaac atgtttgcaa cctttcttta cgccgttttt gtgtgcattc
    18001 acatggtatg atgaaagtgt tcaaacaaat ttctattggg gcatgcgtgt gaccctttct
    18061 aacggggttc actctcggag tcttcatgcg atgagcaagg agtcatgatg ttagatatag
    18121 tcgaactgtc gcgcttacag tttgccttga ccgcgatgta ccacttcctt tttgtgccac
    18181 tgacgctcgg tatggcgttc ctgctggcca ttatggaaac ggtctacgtc ctctccggca
    18241 aacagattta taaagatatg accaagttct ggggcaagtt gtttggtatc aacttcgctc
    18301 tgggtgtggc taccggtctg accatggagt tccagttcgg gactaactgg tcttactatt
    18361 cccactatgt aggggatatc ttcggtgcgc cgctggcaat cgaaggtctg atggccttct
    18421 tcctcgaatc cacctttgta ggtctgttct tcttcggttg ggatcgtctg ggtaaagttc
    18481 agcatatgtg tgtcacctgg ctggtggcgc tcggttcaaa cctgtccgca ctgtggattc
    18541 tggttgcgaa cggctggatg caaaacccaa tcgcgtccga tttcaacttt gaaactatgc
    18601 gtatggagat ggtgagcttc tccgagctgg tgcttaaccc ggttgctcag gtgaaattcg
    18661 ttcacactgt agcgtctggt tatgtgactg gcgcgatgtt catcctcggt atcagcgcat
    18721 ggtatatgct gaaaggtcgt gacttcgcct tcgctaaacg ctcctttgct atcgctgcca
    18781 gcttcggtat ggctgctgtt ctgtctgtta ttgttctggg tgatgaatcc ggctacgaaa
    18841 tgggcgacgt gcagaaaacc aaactggctg ctattgaagc cgagtgggaa acgcaacctg
    18901 cgcctgctgc ctttactctg ttcggcattc ctgatcagga agaggagacg aacaaatttg
    18961 cgattcagat cccttacgca ctgggcatca ttgcaacgcg ttccgtggat accccggtta
    19021 tcggcctgaa agagctgatg gtgcagcatg aagaacgcat tcgtaacggg atgaaggcgt
    19081 actctctgct cgaacaactg cgttctggtt ctaccgacca ggcggttcgt gaccagttca
    19141 atagcatgaa gaaagacctc ggttacggtc tgctgctgaa acgctatacg ccaaacgtgg
    19201 ctgatgcgac tgaagcgcag attcaacagg caaccaaaga ctccatcccg cgtgtagcgc
    19261 cgctgtactt tgcgttccgt atcatggtgg cgtgtggctt cctgcttctg gcaatcatcg
    19321 cgctctcttt ctggagtgtc atccgcaacc gcattggcga gaaaaaatgg cttctgcgcg
    19381 ccgcgctgta cggtattccg ctgccgtgga ttgctgtaga agcgggctgg ttcgtggctg
    19441 aatatggccg ccaaccgtgg gctatcggtg aagtgctgcc gacagctgtg gcgaactcgt
    19501 cactgaccgc aggcgatctc atcttctcaa tggtgctgat ttgcggcctg tataccctgt
    19561 tcctggtggc agaattgttc ttaatgttca agtttgcacg cctcggccca agcagcctga
    19621 aaaccggtcg ctatcacttt gagcagtctt ccacgactac tcagccggca cgctaagaca
    19681 ggagtcgtca aatgatcgat tatgaagtat tgcgttttat ctggtggctg ctggttggcg
    19741 ttctgctgat tggttttgca gtcactgacg gtttcgacat gggggtgggc atgctcaccc
    19801 gtttcctcgg tcgtaacgac accgagcgtc gaattatgat taactccatt gcaccacact
    19861 gggacggtaa ccaggtttgg ctgatcaccg cgggcggcgc actctttgct gcctggccga
    19921 tggtctatgc cgctgcgttc tccggcttct atgtggcgat gatcctcgtg ctggcgtctt
    19981 tgttcttccg tccggtcggt tttgactacc gctccaagat tgaagaaacc cgctggcgta
    20041 acatgtggga ctggggcatc ttcattggta gtttcgttcc gccgctggta attggtgtag
    20101 cgttcggtaa cctgttgcag ggcgtaccgt tcaacgttga tgaatatctg cgtctgtact
    20161 acaccggtaa cttcttccag ttgcttaacc cgttcggcct gctggcaggc gtggtgagcg
    20221 tagggatgat cattactcag ggcgcaacct atctgcaaat gcgtaccgtg ggcgaactgc
    20281 acctgcgtac ccgtgcaacg gctcaggtgg ctgcgctggt gacactggtc tgtttcgcac
    20341 tggctggcgt atgggtgatg tacggtatcg atggttatgt cgtgaaatcg acaatggacc
    20401 attacgcagc ctctaaccca ctgaataaag aagtggttcg tgaagctggc gcatggctgg
    20461 ttaacttcaa caacacgcca attctgtggg ctattccggc actgggtgtg gttcttccgc
    20521 tgctgaccat cctgactgca cgtatggata aagccgcgtg ggcgtttgtg ttctcctccc
    20581 tgacgctggc ctgcatcatc ctgacagccg gtatcgcaat gttcccgttt gtgatgccgt
    20641 ccagcaccat gatgaacgca agtctgacaa tgtgggatgc aacttccagc cagctgacgc
    20701 ttaacgtcat gacctgggtt gcggtggttc tggtaccgat cattctgctc tacaccgctt
    20761 ggtgttactg gaaaatgttc ggtcgtatca ccaaagaaga tattgaacgt aacacccact
    20821 ctctgtacta agtaaggagc taaaaatgtg gtatttcgca tggattctgg gaacgcttct
    20881 tgcctgttcg tttggggtaa tcaccgcgct ggcgcttgaa cacgtcgaat caggcaaagc
    20941 cggtcaagaa gacatctgat gagtaagatt atcgcgacct tgtatgcggt aatggacaag
    21001 cgccccctgc gggcgctttc cttcgtgatg gcgcttctgt tagcaggatg tatgttttgg
    21061 gacccatctc gttttgccgc gaagaccagt gaactggaaa tctggcatgg tttattgctg
    21121 atgtgggccg tctgtgctgg tgtgattcac ggcgtgggct ttcgtccgca gaaggttctt
    21181 tggcaaggga ttttttgccc attgcttgcc gatattgttc tcattgtcgg gctgattttc
    21241 ttcttctttt aaatcagaat tttctttaaa aaattatggg ccgctccagg cccataaatt
    21301 tttacgctcc cttaacttgc cctcattccc aaacctcaat cgcgcgcgta tagtagcagc
    21361 gtttaaaagc tctaactttt gttgcattac cgggatgtaa agtgaataca acgctgtttc
    21421 gatggccggt tcgcgtctac tatgaagata ccgatgccgg tggtgtggtg taccacgcca
    21481 gttacgtcgc tttttatgaa agagcacgca cagagatgct gcgtcatcat cacttcagtc
    21541 agcaggcgct gatggctgaa cgcgttgcct ttgtggtacg taaaatgacg gtggaatatt
    21601 acgcacctgc gcggctcgac gatatgctcg aaatacagac tgaaataaca tcaatgcgtg
    21661 gcacctcttt ggttttcacg caacgtattg tcaacgccga gaatactttg ctgaatgaag
    21721 cagaggttct ggttgtttgc gttgacccac tcaaaatgaa gccttgtgcg cttcccaagt
    21781 ctattgtcgc ggagtttaag cagtgactga catgaatatc cttgatttgt tcctgaaggc
    21841 tagccttctg gttaaactta tcatgttgat tttgattggt ttttcaatcg catcctgggc
    21901 cattattatc cagcggaccc gtattcttaa cgcagcggcg cgcgaagccg aagcgtttga
    21961 agataaattc tggtctggaa tcgaactctc tcgcctctat caagagagtc aggggaaacg
    22021 ggataatctg actggttcgg aacaaatatt ttacagcggg ttcaaagagt ttgtgcgcct
    22081 gcatcgtgcc aatagccatg cgccggaagc cgtagtggaa ggggcgtcgc gtgctatgcg
    22141 tatctccatg aaccgtgaac ttgaaaatct ggaaacgcac attccgttcc tcggtacggt
    22201 tggctccatc agcccgtata ttggtctgtt tggtacggtc tgggggatca tgcacgcctt
    22261 tatcgccctc ggggcggtaa aacaagcaac actgcaaatg gttgcgcccg gtatcgcaga
    22321 agcgttgatt gcgactgcaa ttggtctgtt cgccgcaatt ccggcggtaa tggcttataa
    22381 ccgcctcaac cagcgcgtaa acaaactgga actgaattac gacaacttta tggaagagtt
    22441 taccgcgatt ctgcaccgcc aggcgtttac cgttagcgag agcaacaagg ggtaagccat
    22501 ggccagagcg cgtggacgag gtcgtcgcga tctcaagtcc gaaatcaaca ttgtaccgtt
    22561 gctggacgta ctgctggtgc tgttgctgat ctttatggcg acagcgccca tcatcaccca
    22621 gagcgtggag gtcgatctgc cagacgctac tgaatcacag gcggtgagca gtaacgataa
    22681 tccgccagtg attgttgaag tgtctggtat tggtcagtac accgtggtgg ttgagaaaga
    22741 tcgcctggag cgtttaccac cagagcaggt ggtggcggaa gtgtccagcc gtttcaaggc
    22801 caacccgaaa acggtctttc tgatcggtgg cgcaaaagat gtgccttacg atgaaataat
    22861 taaagcactg aacttgttac atagtgcggg tgtgaaatcg gttggtttaa tgacgcagcc
    22921 tatctaaaca tctgcgtttc ccttgcttga aagagagcgg gtaacaggcg aacagttttt
    22981 ggaaaccgag agtgtcaaag gcaaccgaac aaaacgacaa gctcaagcgg gcgataatta
    23041 tttcagcagt gctgcatgtc atcttatttg cggcgctgct ctggagttcg ttcgatgaga
    23101 atatagaagc ttcagccgga ggcggcggtg gttcgtccat cgacgctgtc atggttgatt
    23161 caggtgcggt agttgagcag tacaaacgca tgcaaagcca ggaatcaagc gcgaagcgtt
    23221 ctgatgagca gcgcaagata aaggaacagc aggctgctga agaactgcgt gagaaacaag
    23281 cggctgaaca ggaacgcctg aagcaacttg agaaagagcg gttagctgct caggaacaga
    23341 aaaagcaggc tgaagaagcc gcaaaacagg ccgagttaaa gcagaagcaa gcggaagagg
    23401 cggcagcgaa agcggcggca gatgctaaag cgaaggccga agcggatgct aaagctgcgg
    23461 aagaagcagc gaagaaagcg gcggcagacg cgaagaaaaa agcagaagca gaagccgcca
    23521 aagccgcagc cgaagcgcag aaaaaagccg aggcagccgc tgcggcactg aagaagaaag
    23581 cggaagcggc agaagctgct gcagctgaag caagaaagaa agcggcagca gagaaagctg
    23641 cagccgacaa aaaagcagca gaaaaagcgg ctgctgataa ggcagcagct gataagaaag
    23701 cagcggcaga aaaagccgcc gcagacaaaa aagcggcagc ggcaaaagca gcggcagaaa
    23761 aagccgccgc agacaaaaaa gcggcagcgg caaaagcagc agctgaaaaa gccgctgcag
    23821 caaaagctgc cgcagaggca gatgatattt tcggtgagct aagctctggt aagaatgcac
    23881 cgaaaacggg gggaggggcg aaagggaaca atgcttcgtc tgccgggagt ggtaatacta
    23941 aaaacaatgg cgcatcaggg gccgatatca ataactatgc cgggcagatt aaatctgcta
    24001 tcgaaagtaa gttctatgac gcatcgtcct atgcaggcaa aacctgtacg ctgcgcataa
    24061 aactggcacc cgatggtatg ttactggata tcaaacctga aggtggcgat cccgcacttt
    24121 gtcaggctgc gttggcagca gctaaacttg cgaagatccc gaaaccacca agccaggcag
    24181 tatatgaagt gttcaaaaac gcgccattgg acttcaaacc gtaatcgcga tgttgactgt
    24241 tccgacggtc aacatcaggc accggttgcc acggggttct ggtagttttg tgtattttag
    24301 tttgttaaca ttctgctaaa ttatcgtggg ccatcggtcc agataaggga gatatgatga
    24361 agcaggcatt acgagtagca tttggttttc tcatactgtg ggcatcagtt ctgcatgctg
    24421 aagtccgcat tgtgatcgac agcggtgtag attccggtcg tcctattggt gttgttcctt
    24481 tccagtgggc gggccctggt gcggcacctg aagatattgg cggcatcgtt gctgccgact
    24541 tgcgtaacag cggtaaattt aatccgttag atcgcgctcg tctgccacag cagccgggta
    24601 gtgcgcagga agtacaacca gctgcatggt ccgcgctggg tattgacgct gtagttgtcg
    24661 gtcaggtcac tccgaatccg gatggttctt acaatgttgc ttatcaactt gttgacactg
    24721 gcggcgcacc gggtactgta cttgctcaga actcgtacaa agtgaacaag cagtggctgc
    24781 gctatgctgg tcataccgcc agtgatgaag tgtttgaaaa actgaccggc attaaaggtg
    24841 cgttccgtac ccgtattgcc tacgttgttc agaccaacgg cggtcagttc ccgtatgaac
    24901 tgcgcgtatc tgactatgac ggttacaacc agtttgtcgt tcaccgttca ccacagccgc
    24961 tgatgtctcc ggcgtggtca ccagacggtt ctaaactggc ttatgtgacc ttcgaaagcg
    25021 gtcgttccgc gctagttatc caaacgctgg caaacggcgc tgtacgtcag gtggcttcat
    25081 tcccgcgtca caacggtgcg cctgcattct cgccagacgg cagcaaactg gcattcgcct
    25141 tgtcgaaaac cggtagcctg aacctgtacg taatggattt ggcttctggt cagatccgcc
    25201 aggtgactga tggtcgcagt aacaataccg aaccgacctg gttcccggac agccagaacc
    25261 tggcatttac ttctgaccag gccggtcgtc cacaggttta taaagtgaat atcaacggcg
    25321 gtgcgccaca acgtattacc tgggaaggtt cgcagaacca ggatgcggat gtcagcagcg
    25381 acggtaaatt tatggtaatg gtcagctcca acggtgggca gcagcacatt gccaaacaag
    25441 atctggcaac gggaggcgta caagttctgt cgtccacgtt cctggatgaa acgccaagtc
    25501 tggcacctaa cggcactatg gtaatctaca gctcttctca ggggatggga tccgtgctga
    25561 atttggtttc tacagatggg cgtttcaaag cgcgtcttcc ggcaactgat ggacaggtca
    25621 aattccctgc ctggtcgccg tatctgtgat aataattaat tgaatagtaa aggaatcatt
    25681 gaaatgcaac tgaacaaagt gctgaaaggg ctgatgattg ctctgcctgt tatggcaatt
    25741 gcggcatgtt cttccaacaa gaacgccagc aatgacggca gcgaaggcat gctgggtgcc
    25801 ggcactggta tggatgcgaa cggcggcaac ggcaacatgt cttccgaaga gcaggctcgt
    25861 ctgcaaatgc aacagctgca gcagaacaac atcgtttact tcgatctgga caagtacgat
    25921 atccgttctg acttcgctca aatgctggat gcacatgcaa acttcctgcg tagcaacccg
    25981 tcttacaaag tcaccgtaga aggtcacgcg gacgaacgtg gtactccgga atacaacatc
    26041 tccctgggtg aacgtcgtgc gaacgccgtt aagatgtacc tgcagggtaa aggcgtttct
    26101 gcagaccaga tctccatcgt ttcttacggt aaagaaaaac ctgcagtact gggtcatgac
    26161 gaagcggcat actccaaaaa ccgtcgtgcg gtactggttt actaagagaa ttgcatgagc
    26221 agtaacttca gacatcaact attgagtctg tcgttactgg ttggtatagc ggccccctgg
    26281 gccgcttttg ctcaggcacc aatcagtagt gtcggctcag gctcggtcga agaccgcgtc
    26341 actcaacttg agcgtatttc taatgctcac agccagcttt taacccaact ccagcaacaa
    26401 ctttctgata atcaatccga tattgattcc ctgcgtggtc agattcagga aaatcagtat
    26461 caactgaatc aggtcgtgga gcggcaaaag cagatcctgt tgcagatcga cagcctcagc
    26521 agcggtggtg cagcggcgca atcaaccagc agcgatcaaa gcggtgcggc ggcatcaacg
    26581 acgccgacag ctgatgctgg tactgcgaat gctggcgcgc cggtgaaaag cggcgatgca
    26641 aacacggatt acaatgcagc tattgcgctg gtgcaggata aatcccgcca ggatgacgca
    26701 atggtggcat ttcagaattt catcaaaaat taccctgatt caacttacct gccaaacgcc
    26761 aattattggc tgggtcagtt aaactacaac aagggtaaaa aagatgatgc ggcgtactat
    26821 tttgcttcgg tagtgaaaaa ctatccgaag tcaccaaagg ctgctgatgc gatgtttaaa
    26881 gtcggcgtca tcatgcagga caaaggtgac accgcaaaag cgaaagccgt ataccagcag
    26941 gttatcagta aataccctgg tactgatggc gctaaacagg cacaaaaacg tctgaacgcg
    27001 atgtaatgca taacacacga ccagaagtcg cattatttct ggtcgtgtcg tgcgaatcat
    27061 aagcagttga gtgatctgca tcgaaatttt tgttgcgctc aagtctgaaa tcagtaatat
    27121 atgccgccgt tgccacggga tatcaaacaa accgaaagca acgaaaaagt gggtcgttag
    27181 ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac
    27241 ccaccagttt tagcatcgaa gacagatgtt aagcgtgtag gataacgttg cgtcagcaac
    27301 ggcccgtagg gcgagcgaag cgagtcatcc tgcacgaccc accactaatt acggtgggtt
    27361 cggtggaagt agtttgtagt atccagcgca gtatcgggtg attagctcag ctgggagagc
    27421 acctccctta caaggagggg gtcggcggtt cgatcccgtc atcacccacc actcgggtcg
    27481 ttagctcagt tggtagagca gttgactttt aatcaattgg tcgcaggttc gaatcctgca
    27541 cgacccacca atcttaaaga ttggccccga gtagaaatct ttcaggtaac acccgtatgg
    27601 gtcgttagct cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc
    27661 tgcacgaccc accatcctga atgattaaag cagcataatc ccgcaagggg tcgttagctc
    27721 agttggtaga gcagttgact tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca
    27781 ccaatgtaaa aaagcgccct aaaggcgctt ttttgctatc tgagatattc aaagattcga
    27841 acctgcagca ggttcgagtt gagcgcagcg aaacaacgga gccgctcgcg gcgacggccc
    27901 gaagggcgag cgaagcgagt catcctgcac gacccaccaa tgtaaaaaag cgccctaaag
    27961 gcgctttttt gctattcagg catcctcaat ttcactttgt aaacctgatg acatcgtcag
    28021 agcttactgt gcaagcaact ctacgtcggt ggaattaggc gtaattcgag tgacttttcc
    28081 ccaccattcg actatcttgt ttagcatata aaacaaatta cgccgatagt agcgaatatt
    28141 acgctaatgt cggttttaac gttaagcctg taaaacgaga tggtaagatg agcgtgatgt
    28201 ttgatccaga cacggcgatt tatcctttcc ccccgaagcc gacgccgtta agcattgatg
    28261 aaaaagcgta ttaccgcgag aagataaaac gtctgctaaa agaacgtaat gcggtgatgg
    28321 ttgcccacta ctataccgac cccgaaattc aacaactggc agaagaaacc ggtggctgta
    28381 tttctgattc tctggaaatg gcgcgcttcg gtgcaaagca tcccgcttct acgttgttag
    28441 tcgctggggt gagatttatg ggagaaaccg ccaaaattct cagtccggaa aaaacaattc
    28501 tgatgctgac acttcaggct gaatgttcac tggatctcgg ctgccctgtt gaagaattta
    28561 acgcattttg cgatgcccat cccgatcata ctgtcgtcgt ctacgccaac acttctgctg
    28621 cggtaaaagc gcgcgcagat tgggtggtaa cttcaagcat tgccgtcgaa cttattgatc
    28681 atctggatag ttttggtgaa aaaatcatct gggcacccga caaacatctg gggcgttacg
    28741 tgcaaaaaca gacgggcgca gacattttat gctggcaggg tgcctgtatt gtgcatgatg
    28801 aatttaagac tcaggcgtta acccgcttgc aagaagaata cccggatgct gccatactgg
    28861 tgcatccaga atcaccacaa gctattgtcg atatggcgga tgcggtcggt tccaccagtc
    28921 aactgatcgc tgctgcgaaa acattgccac atcagcggct tatcgtggca accgatcggg
    28981 gcattttcta caaaatgcag caggcggtgc cagataaaga gttactggaa gcaccaaccg
    29041 caggtgaggg tgcaacctgc cgca
//
LOCUS       DAEEKQ010000033        28896 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.033, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000033 DAEEKQ010000000
VERSION     DAEEKQ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28896
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.033"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(218..1129)
                     /gene="gsiD"
                     /locus_tag="KLK57_001464"
     CDS             complement(218..1129)
                     /gene="gsiD"
                     /locus_tag="KLK57_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HBE0891866.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1132..2052)
                     /gene="gsiC"
                     /locus_tag="KLK57_001465"
     CDS             complement(1132..2052)
                     /gene="gsiC"
                     /locus_tag="KLK57_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HBE0891867.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGLEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2070..3608)
                     /gene="gsiB"
                     /locus_tag="KLK57_001466"
     CDS             complement(2070..3608)
                     /gene="gsiB"
                     /locus_tag="KLK57_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HBE0891868.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3628..5499)
                     /gene="gsiA"
                     /locus_tag="KLK57_001467"
     CDS             complement(3628..5499)
                     /gene="gsiA"
                     /locus_tag="KLK57_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HBE0891869.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5486..6451)
                     /gene="iaaA"
                     /locus_tag="KLK57_001468"
     CDS             complement(5486..6451)
                     /gene="iaaA"
                     /locus_tag="KLK57_001468"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HBE0891870.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCIGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6655..7890
                     /gene="moeA"
                     /locus_tag="KLK57_001469"
     CDS             6655..7890
                     /gene="moeA"
                     /locus_tag="KLK57_001469"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HBE0891871.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7890..8639
                     /gene="moeB"
                     /locus_tag="KLK57_001470"
     CDS             7890..8639
                     /gene="moeB"
                     /locus_tag="KLK57_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HBE0891872.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLIVGLGGLGC
                     AASLYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     TSGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8818..9480)
                     /gene="fsa"
                     /locus_tag="KLK57_001471"
     CDS             complement(8818..9480)
                     /gene="fsa"
                     /locus_tag="KLK57_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HBE0891873.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWQGAFGRT
                     SI"
     gene            9611..10510
                     /locus_tag="KLK57_001472"
     CDS             9611..10510
                     /locus_tag="KLK57_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HBE0891874.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMAMALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10516..12948
                     /locus_tag="KLK57_001473"
     CDS             10516..12948
                     /locus_tag="KLK57_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HBE0891875.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLAIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQTLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13094..13909
                     /gene="ybiV"
                     /locus_tag="KLK57_001474"
     CDS             13094..13909
                     /gene="ybiV"
                     /locus_tag="KLK57_001474"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HBE0891876.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKAVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14061..15326
                     /locus_tag="KLK57_001475"
     CDS             14061..15326
                     /locus_tag="KLK57_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003570920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HBE0891877.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSNMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGIDS"
     gene            complement(15293..16453)
                     /locus_tag="KLK57_001476"
     CDS             complement(15293..16453)
                     /locus_tag="KLK57_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000505360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0891878.1"
                     /translation="MGFFAHENSVISELEADHKMDKKLKIADIAARTGLSPSTVSRVL
                     AGKANTSARAREQVLACARELGVMDGMAAGRMLLNNLIIFAPQRAFDQRSDIFYYRVI
                     QSISKALSPHEVRLRYCALEEFDSDPALFLSRMNEADTQAAILLGIDDPHIHDLAADL
                     AKPCVLINCRDERMRLPAIAPDHRLIGAFAARYLFEMGHREVMNVMCLRRYTMEQRFA
                     GIKESWRQHNLAFHHDRHLLNISSFSAKEAEEKVGAWLDAANGKTLPTAFLVGGDFMA
                     AGTVNALHRRGLRVPQDVSVMSIDGFNLAAIEDVPLTAVHVPRDELGNEAVQMLQQRL
                     IRPDAPVGSLLLHGRLAVRESVRRVRPGKGNTAVEREGLYDTQLSIPNARLP"
     gene            16691..17992
                     /locus_tag="KLK57_001477"
     CDS             16691..17992
                     /locus_tag="KLK57_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0891879.1"
                     /translation="MSQGINNDMTASKPRHIVKNLRWWILVLFLLGVTVNYITRNSLG
                     ILAPELKSSLGITTEQYSWIVGAFQLAYTLFQPLCGWLIDVIGLKLGFMICAGLWALA
                     CLMHAGAGNWGHLALLRFFMGGAEAAATPANAKTIGEWFPKSERPIAAGWAGVGFSIG
                     AMLAPPIIYFAHASFGWQGAFMFTGILAMLWVVLWWAFYHSPDKHPNLSKAELDFIRQ
                     DNEAPPVKLPFLTALKTVGKNKRFYGIAIPAFMAEPAWAVMSFWVPLYLSKTYGMDLK
                     QIALFAWLPFLAADLGSIASGYLTKLYTRLFGLSRVNSVVASSVSGAFLMVSLAVMAF
                     TNNPYIAIVLISIGGFGHQIISCMLSALVVESFDKGQMATVNGMRGSAAWIASFLFSL
                     IIGVTADKIGFNPLFIAMGFFDLIGAFFLVTFIAERRAKRA"
     gene            18007..20370
                     /locus_tag="KLK57_001478"
     CDS             18007..20370
                     /locus_tag="KLK57_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HBE0891880.1"
                     /translation="MKTLKNWTLHKQSAHHVELLVDGQHILCLYVLEENLFRVLLKRK
                     GELALDRTWSIAPAQDVPWEGRSREDLSGFSLPAWTVIQHDDTVVIATESLRATVHQP
                     LWLEWHYRNNEGEWLPLVNDRPTSAYLLNAHGDGVAHYQSRRKDERFYGLGDKAGDLQ
                     RNGKRYEMRNLDAMGYNAVSTDPLYKHIPFTITRRDDISFGLFYDNLSSCWLDLGNEI
                     DNYHTAYRRWQAEAGDIDYYLFTGERVLDITKAFVRLTGKTLFGPKWSLGYSGSTMHY
                     TDAPDAQNQLMNFIRLCEEHAIPCDSFQLSSGYTSINGKRYVFNWNYDKVPQPKVMSQ
                     AFHNAGLHLAANIKPCLLQDHPRYNEVAERGLFIRDSETDEPERSSFWDDEGSNLEFT
                     NPQTIQWWQEGVTTQLLEMGIDSTWNDNNEFEVWDGEARCHGFGQTIAIKHIRPVMPL
                     LMMRASMEAQQRFAPEKRPYLISRSGCAGMQRYVQTWSGDNRTSWDTLRYNTRMGLGM
                     SLSGLYNLGHDVGGFSGDKPDPELFVRWVQNGVMHPRFTIHSWNDDHTVNEPWMYPGV
                     TPAIRSAIELRYRLLPYFYTLMWQAYADDEPMLRPTFLDHEHDVQTFEECDDFLLGRD
                     ILVASVVEQGERQRRVWLPDNETGWYDFYNGEWFSGGQWITLDAPLEKLPLMVRAGAG
                     LPLSKRITYVSAEQDDTRELKLFPLKGVGTTSGLLFEDDGESWGYQTGNALWVEWEMV
                     CDGATINLKVNARGDYRPAWSALKVSLPLEEKRTLLVNGVEGSEWMR"
     gene            complement(20550..22145)
                     /locus_tag="KLK57_001479"
     CDS             complement(20550..22145)
                     /locus_tag="KLK57_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HBE0891881.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPEILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERAIDFSGNYEDYLRSKGIDA"
     gene            22364..23284
                     /gene="ldtB"
                     /locus_tag="KLK57_001480"
     CDS             22364..23284
                     /gene="ldtB"
                     /locus_tag="KLK57_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBE0891882.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(23343..24461)
                     /locus_tag="KLK57_001481"
     CDS             complement(23343..24461)
                     /locus_tag="KLK57_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HBE0891883.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(24458..24925)
                     /gene="mntR"
                     /locus_tag="KLK57_001482"
     CDS             complement(24458..24925)
                     /gene="mntR"
                     /locus_tag="KLK57_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HBE0891884.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            25111..25239
                     /gene="mntS"
                     /locus_tag="KLK57_001483"
     CDS             25111..25239
                     /gene="mntS"
                     /locus_tag="KLK57_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HBE0891885.1"
                     /translation="MNEFKKCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            25511..27094
                     /gene="opgE"
                     /locus_tag="KLK57_001484"
     CDS             25511..27094
                     /gene="opgE"
                     /locus_tag="KLK57_001484"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HBE0891886.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(27143..27658)
                     /gene="ompX"
                     /locus_tag="KLK57_001485"
     CDS             complement(27143..27658)
                     /gene="ompX"
                     /locus_tag="KLK57_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HBE0891887.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(27711..27776)
                     /locus_tag="KLK57_001486"
     CDS             complement(27711..27776)
                     /locus_tag="KLK57_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891888.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            28011..>28896
                     /gene="rhtA"
                     /locus_tag="KLK57_001487"
     CDS             28011..>28896
                     /gene="rhtA"
                     /locus_tag="KLK57_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HBE0891889.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         6961 a         7950 c         7287 g         6698 t
ORIGIN      
        1 ttatcagcct tatctttatc ttttcgtata aactcagcat gttaatacat ttaacaaaat
       61 aactatctga acgctgtgcc gctgcaaaaa tagtcccttg ctgcggcttt ttaaccaaat
      121 tacccgattc tacaaaatca gcagaaagtt acaacacttg cttagggcaa taatttcctg
      181 tgaggtgatt accctttcaa gcaatattca aacgtaacta tcctttaatt ttcggatcca
      241 gcgcatcgcg taaaccatcg cccaacaaat tgaacgccag tacggtcaga aaaatagcca
      301 gggccggaaa aacagcgaca tgcggcgcga taaccatatc cgctcgtgcc tcattgagca
      361 ttgctcccca ctctggtgtc ggcggctgcg caccgaggcc gagaaatgac aggctggcgg
      421 cagagataat cgaggtgcca atgcgcatgg tgaaaaacac cacgatagac gagacggtcc
      481 caggcaagat atgacgcaat aaaatggtca tatcgctggc accaatactg cgtgctgact
      541 caataaaggt ttgctgtttc aacaccagcg tgttgccgcg caccagtcgg gcaaacgcgg
      601 ggatggaaaa aatggcgact gcgataatca cgttagcaat gccgcttccc aacaccgcaa
      661 caacagcgat cgccagtaaa atacccggga aggcaaacag cacatcgcaa atgcgcatga
      721 tcagccgatc ccaccagcct tcataatatc cagccaacaa gcccagcaac gtcccgatcg
      781 ccgcaccgat aaacacggca aacacgcccg ccgccagcga gatttgcgca ccaaccagga
      841 cacggctgaa aatgtcgcgc cccagtgaat cgacgccaaa ccagtgctgc aaagaaggtc
      901 cgttattcag attgtcataa tcaaaataat tttcggcgtc atagggagcg atccagcgtg
      961 caaaaatggc caccacaatc aataaaataa cgaataatgc ggcggtcatc gccatatgct
     1021 gacggcgaaa tcgtcgccag aattcatgcc acggtgtacg cacctggtca ggtttgacca
     1081 gtggcatggc gtttaacacc gcctgacgtc gccagttaaa tagtcgcatc cttacttgta
     1141 cctgatagcc gggttaatgg cggcgtaaag cacatccacc actaagttga taagaataaa
     1201 ttccagcgag aaaagcagaa tttccgcctg aatcaccggg taatcgcgca tttctacgga
     1261 gtcaacgagt aagcgtccaa gtccgggcca gttgaaaact ttctccacca cgatagaacc
     1321 accgagcaaa aagccaaact gtaagcccat catggtcact accgggatca tcgcgttacg
     1381 tagcccgtgt ttgaggacaa cccaggtttc gctcacccct ttcgccctcg cggtacgcat
     1441 ataatcttcg cttaaaacat cgacaaacga cgcgcgggta aagcgcgcca tcacggctgc
     1501 cactgccgcg ccgagcgtca gggagggtaa aatgtagtgc tgccaactgt ctgctcccac
     1561 ggtaggcagc cagcccagtt caacggagaa tacctgaatt aaaagcatcc ccagcgcaaa
     1621 tgccggaaac gagatccccg acaccgcaat ggtcatactc aatcgatccg gccaacgatt
     1681 acgccagacg gcggcgataa ttcccgccgc cataccaaat atcaccgccc agaccatact
     1741 ggttatggtc agccacagcg ttggcataaa gcggctggca atctcatcgg caaccggacg
     1801 acgcgacacc atcgacaggc caaaatcccc ctgcacagca ttgctgatat agtgccagaa
     1861 ctggtgatac agcggctgat ccaaccccag ctgctgacgc accagttcta taacctgcgc
     1921 atcagcttcg agcccggcaa tcaatcgcgc cggatcgccg ggcagcatat gcacaaataa
     1981 aaacaccagc accgagacga taaacagcgt cggaatcaac cccagtaagc gtttgataac
     2041 gtaattaagc attccactcc ctgcgttgct tattgcaaat ccgcgtcttc aaagctgaag
     2101 ccggtgtctg gcatgatcca aaaaccggtc aggtttttac tgtgtgccga caccagtttt
     2161 tctaccacca gcgggatcca cggcgactct tgccagatga tatcctgcgc cgccttatac
     2221 aagcgggtct tttccgccgg atcattagtt ttcagtgcct gagccaggaa gtcgtccacc
     2281 tgtttattgc tgtaaaatgc ggtattaaac agcgtcggtg gccagttttg cgaggcgaac
     2341 agtggcgata gcgcccagtc cgcttcgccg gttgaagccg accagccagt gtagaacatc
     2401 cgcacgccgc tctctttttg ccctttacct tcaacttctg ccgcccgctg tccggcatcc
     2461 atcgcggtca cctgggcttt aatcccgacc tgcgctaact gctgctgggt aaattgcagc
     2521 actttctgcg cggtgctgtg gttatgtgac gaccacagcg tggtactgaa accgttggga
     2581 tatcccgcct ctttcagtaa ttcgcgcgct ttcactggat cgtaaggcca cggtttataa
     2641 ctttgcgcgt aggcgatact tggcggtacc acaccagtag ctggcgttgc atagcccgca
     2701 aaggcaactt tcaccagcgc cggacggtta atggcgtaat tcagcgcctc acggaccttc
     2761 gggttatcga acggcttttg cgtcacgttc atactgatat aacgctgcat aattgacgga
     2821 ctggccatca actcgatatt tttgtttttc tccagcagtg tggcctgctc gtagggaatg
     2881 gggaaagcaa actgcgcttc accggtttgc agcattgccg cgcgggtgtt gttatccgcc
     2941 accggacgcc aggttatgct gtccagtttg ggcaatcctg gctgccagta acccgcgaat
     3001 tttttcacct tcacaaaatc ggtctgattc caggtatcca gttcatacgg tccggttccc
     3061 accggatgaa aaccaatctc cttgccatat ttttccagcg ctgccggtga aatcatcgcg
     3121 gtcgccggat gggcaagaat attaataaac gctgagaacg gctgtttgag ggtaatcttt
     3181 accgttgtcg gatcgatcgc ctccgttttg gcaatattct tatacaggtt atagcgttta
     3241 agatgattcg ccggatcgct ggcccggtcc agattcgctt ttaccgccac tgcgttgaaa
     3301 tcggtgccat cctggaattt aatgccttcc cgcaatttca cggtgtaagt aatgccgtca
     3361 tcggaaacgg tataactctc cgccagcacg tttttcagtt tcatctcttt atccagaccg
     3421 aacagcccct ggtaaaacga tttcgctacg gcctgagata acgtatcatt cgcgtcatac
     3481 ggatcgagcg tggtgaaatt cgatcctacc gccaccacca catctttggc agcgaatgca
     3541 caagatgcca tcaacgctgt cgcaatgccc agcgccacta acccactacg gtgtacagct
     3601 cttgccattt tattctccgc ctgaatgtta tctacgcatg aatgcgtatt ctgattgtgg
     3661 ttgtgcgacg taatgccccg gcccgacgca ttgcaacgag acggctgcca cctcttcgcc
     3721 acgcagatga atattgctgg gaagatcgtc cgacagcagt acacgctgcg gtcgttgtcg
     3781 ggacggttca gcgaccggaa ctgccgccag taatttacgc gtataaggat gctgcgggtt
     3841 ttcgaagacc gcgcgccgtg gaccaatttc aacaatttgc ccgagataca tcaccgccac
     3901 acgatgacta atccgctcta ccacggccat atcgtgggag ataaacagat acgcaatgcc
     3961 gaaatcacgc tggagatcga gcaacaagtt gataatctgc ccgcgaatag aaacatccag
     4021 cgccgaaacg gcttcgtcgg caatgatcac ttttggattc aatgccaacg cgcgagcaat
     4081 gcagatgcgc tggcgctgac cgccggaaaa ctcatgcggg taacgccagg catgttcagg
     4141 taacaggccc acgcgctcca gcaaccacgc aacgcgtgca gccgcgtctt tacctggcaa
     4201 taaaccgtgt acacgcagcg gttcgataat cgaatcaccg atggtctgac gtgggtccag
     4261 cgaagcgtaa gggtcctgaa aaataaactg aatatcccgg cgcaatgcct gaagtttgcc
     4321 gggtgacaag gtatcgattc tctgaccgtt aaagataatt tcgccgccct gcgattcgac
     4381 caggcgcagc aacgcccgcc cggtagtgga tttaccgctg ccagactcgc ccaccagcga
     4441 tagcgtttcg ccaggccaga gatcaaaact gactttctca acggcatgca cttcccgcgt
     4501 tacgcgattc aacaaaccgc tgcgcaaagg gaaacgggtg acaagattac gcactcgtaa
     4561 aacaggttcg ccatccacca ccgttttctg ctcgatgggg ggggcctgtt tcgctggatg
     4621 ttcaagcgat atcaacggga aacgtcgggg ataatccaac cctttcatcg caccaagttg
     4681 cggaacagca gctaacagcg cacgggtgta aggatgttgc ggtgcatgaa aaatctgttc
     4741 gacggtaccc gtttccaccg cctcgccctg atacatcacc agtacccgat cggcaatctc
     4801 tgccaccacg cccatatcgt gagtgataaa gataacgccc atcgacatct ctttttgcaa
     4861 tactttgatt aattgcagga tctgcgcctg aatagtgaca tccagcgcgg tggttggctc
     4921 atcggcaatc agcaccgccg ggcggcatga cagcgccatc gcaatcatca ctcgctggcg
     4981 catcccgcca gagagttgat gcggataacg tgaaagaatg gtttgtgcct caggaatgcg
     5041 tacctgatcc agcatccgct tcgcctcgac catcgcttct tcacgactgg cgttctgatg
     5101 cagacgaatt gattcggcaa tctgttcacc cacagtaaat accgggttca gcgatgtcat
     5161 cggctcctga aatatcatcg ccatatccgc accgcgaaca tggcgcattt gtgcagcgct
     5221 ctgctcgcta agttcaatca cttcgcgact gcgccgctgc aacagcattt tatcgcactg
     5281 tactaaaccg cccgcctgtt ccaacaggcg catcaatgcc aacgcagtca ctgacttacc
     5341 ggagccggat tcgccaacaa ttgccagcgt ctcaccgcgt tgcagactaa aagagagatt
     5401 gcggaccgca gctattttct gctggtcctg cataaaggca atattcagat tttcaaccgc
     5461 cagcacatta ccggcatcaa gttcatcact gtgtggcaac ggtgtcccct ttttcacggt
     5521 agataccggt ggttggcgta tcgcctgcgt agccccaggc gcgatacatt ccttcggtgt
     5581 taaacggtag cgcgacattc ccttcatggt cgatagcgat taagccaccg ctaccgccaa
     5641 gcgcagggag tttttccatt actacccgct cgcaggcttc cgcgagactt aatccgccgt
     5701 aatccattaa cgcggcgatg tcatatgccg ccagcgcgcg gatgaagact tcgcccgtgc
     5761 cgatacaaga aaccgccaca ctggcgttat tggcgtagca tccggcaccc actaagggac
     5821 tatcgccaac tcgtccgggt aatttattgg tcattccgcc tgtggacgtg gctgccgcca
     5881 aattgccgtc taaatccaac gccacggccc ccacggtgcc cattttctgt ttttcatcca
     5941 gtggcgcacc gctatggtcg aggacggttg ccccttcctt gcgcgctgcc agtagttgtt
     6001 cataacgcaa aggcgtggag aaaatctccg gcgagacgcg ctccatgcca cgagcaaacg
     6061 caaaattttc tgccccttcg ccaatcatca tcacatgcgg gctttgctcc atcaccagcc
     6121 gggcggcaag aaccggatta cgcagatgac taacgcccgc caccgcaccg gctttcaggg
     6181 tgttaccatc catcacacag gcgtccagtt catgggtttc atcacgcgta aagacagcgc
     6241 caattccggc gttaaacagt ggacactctt ccagcagacg caccgcttcc gtcaccacat
     6301 ccagcgcact ttcgcccgct tccagcattt tctgcccggt ttcaacaatg gcagacaacg
     6361 cctcaatgta gcgtaattcc tgttgcagac tcatctgcgc gcggctaatt gcacctgcgc
     6421 caccatgaat tgcaatgact gctttgccca tagtattcgt ccattaaggg tggctcgcca
     6481 cttttgctat aaatatcaga atttttatga attactcatt caatttttga atatagaaag
     6541 atggatatgg catgtaaagg caggagccaa cgctacgccc gaggtggcat acagtctgtg
     6601 gcgtttttct gacataatag gcaaattcga ttttgcctcc gcaggagtgt tttcatggaa
     6661 tttaccaccg gattgatgtc gctcgacacc gcgcttaatg agatgctttc tcgcgtcacc
     6721 ccactgaccg cccaggaaac gctgccactg gtacagtgtt ttggtcgtat tctggcgagc
     6781 gatgtcgttt cgccgcttga tgttccgggg tttgataact ccgcaatgga cggctacgcg
     6841 gtgcgtttag ccgatattgc ctccgggcaa ccgctgcccg ttgccggtaa atcctttgcc
     6901 ggtcagccgt atcatggtga atggcctgcg ggcacctgca ttcgtattat gaccggtgcg
     6961 ccggtgccgg aaggctgcga agcggtggtg atgcaggagc agactgaaca aacggacaat
     7021 ggcgtgcgtt ttactgctga agtgcgtagc gggcaaaata ttcgccgtcg cggtgaagat
     7081 atctctgcag gtgcggttgt tttcccggcg ggaactcgcc tgactaccgc agagctgcca
     7141 gtaattgctt cactggggat tgccgaagtt ccggtgattc gtaaagtgcg tgtagcgctt
     7201 ttttctaccg gtgatgaact ccagttgccc ggtcagccgc tgggcgacgg ccaaatctac
     7261 gacaccaacc gtctcgccgt acacctgatg ttagaacagc tgggttgcga ggtaatcaac
     7321 ttagggatta tccgcgacga tccccatgcc ctgcgcgccg catttattga agccgacagc
     7381 caggcggatg tagtgatcag ttccggcggt gtttcagtgg gtgaagcgga ttacaccaaa
     7441 acgattcttg aagagctggg ggagatcgcc ttctggaagc tggcgattaa accaggtaaa
     7501 ccgttcgcgt tcggtaaact cagcaatagc tggttctgcg gcctgccggg caacccggtt
     7561 tcagcgacgc tgaccttcta tcaactggta cagcctttgc tggcaaaact aagcggcaac
     7621 accgccagcg gcctgcccgc gcgccagcgc gtacgcacag cgtcccgcct gaaaaaaacg
     7681 ccaggacgtc ttgatttcca gcgcggcgtg ctgcaacgca acgccgacgg tgaacttgaa
     7741 gtgacgacca ccggacatca gggttcacat atatttagct cctttagtct cggcaactgc
     7801 tttatcgtgc tggaacgcga tcgcggcaat gtggacgtgg gcgaatgggt ggaagtagaa
     7861 ccgtttaacg cgttgttcgg aggcctgtaa tggcggaact cagcgatcag gagatgctgc
     7921 gctacaaccg gcaaatcatt ctgcgcggtt ttgattttga cggtcaggag gcgctaaaag
     7981 attctcgtgt gctgatcgtc ggcctcggtg gactcggctg cgcagcttcg ctgtatctgg
     8041 caagcgccgg tgtcggcaac ctgacgctgc tcgacttcga cacggtttcg ctctcgaatc
     8101 tgcaacgcca gacactgcac agtgatgcca cggtcgggca accgaaggtg gaatccgccc
     8161 gtgacgccct gacgcggatc aacccacata tcgcgattac gccagtcaat gcactgctag
     8221 atgacgcaga acttgcagca ttgattgctg aacacgatct ggtgctcgac tgtacggata
     8281 acgttgcggt acgtaatcaa ctgaacgcag gctgttttgc cgcgaaggtg ccgctggttt
     8341 ccggcgcggc aattcgtatg gaagggcaaa tcacggtctt cacttatcag gacggcgaac
     8401 cgtgctatcg ctgccttagc cgtttgtttg gcgagaacgc attaacctgc gtagaagcag
     8461 gcgtgatggc accgttgatc ggcgtaattg gttcgttgca ggcaatggaa gcgatcaaac
     8521 tgctggcagg ttatggcaaa ccgaccagcg ggaaaatcgt catgtacgat gcgatgacct
     8581 gtcagtttcg cgaaatgaaa ctgatgcgca atccggggtg tgaggtgtgc gggcagtaat
     8641 tgccacctcc aggcctgata agacgcgaca agcgtcgcat caggcatttg ctccagacgc
     8701 cagatgcggc gtgaacgcct tatctggcct acggttcggg cacagatttc caagcgcggc
     8761 aggcatcggt gcactccgcc acgtcatcag caatttatga ggatgcagaa cgtccggtta
     8821 aatcgacgtt ctgccaaacg ctccctgcca gtcctgctca aacttcgcca cagcggcttc
     8881 caccgccgga tagctaatca tctgttgtgc cacatccagt ggcagagtaa ttgattcaca
     8941 tcctgccagt aagcagtcca gcgcctgacg cggggttttg aaactcgctg ccagcacttt
     9001 cgcctgcggc gcatgcattt tcaataactg gtgtaagtcg gtcacagtct gaatgccgct
     9061 accgccctga gcatcaatac gattaacgta aggcgcaaca tattccgcac ctgccagcgc
     9121 cgacagcaac ccttgtgctg cgccatatac cgcggttccc agcgttggaa tcccttccgc
     9181 ttttaacatc ttaatagctg ccagcccctc ggcggtcacc ggaactttca ccacgatatc
     9241 cgcaataata gaacgcaatt tacgtgcgtc attcaccatc ccttcagcag tggtagccat
     9301 tacctgggca aacagacgcc cctgaccgcc catcgcttca tgaagttgcg gaagcacaac
     9361 atccagcggt tttttacccg cggcgataat gcttgggtta gtggtcacac ccgccagcgg
     9421 aaaaatacgt gacagcgcct tcaccgcaac aacgtctgaa gtatccagat acagttccat
     9481 aaccatcctc aaaatgtact ttttgtctga atgtagacta tcacgggaaa actctctgaa
     9541 cagttgacgc acatcaatct aactttcatt cgaaagtatt ttaatgtttg aacgaaacag
     9601 agaggcgact atgattttca atattcagcg ctactcgacc catgatggcc ccggtatccg
     9661 cacggtcgta tttcttaaag gctgttcgct gggctgccgc tggtgtcaga acccggaaag
     9721 ccgcgcccgc acgcaggatc tgctgtatga cgcacgactg tgtctggaag gctgcgagct
     9781 gtgcgctaag gccgcgccgg aagtgattga gcgcgcgctg aatggtttgc ttattcatcg
     9841 ggaaaagtta accccggagc atctgacggc gttaaccgac tgctgtccga cacaggcatt
     9901 aaccgtgtgt ggtgaagtga aaagcgttga ggagatcatg acgaccgttc tgcgcgataa
     9961 accgttttac gatcgcagcg gcggcggttt aacgctttcg ggtggtgagc cctttatgca
    10021 gccggaaatg gcgatggcgc tactgcaagc cagccacgag gcaggcattc atactgcggt
    10081 agaaacctgt ctgcatgtgc cgtggaaata tatcgcccct tctctgccct atatcgatct
    10141 gtttcttgcc gatttaaaac acgttgccga cgcgccgttt aaacagtgga ccgacggtaa
    10201 cgccgccaga gtgctggata acctgaaaaa actcgccgca gcgggcaaaa aaatcattat
    10261 ccgcgtgccg ctgattcagg gctttaatgc cgacgaaacc tctgtaaaag ctattaccga
    10321 ttttgccgcc gacgagctgc acgttggcga aattcatttc ctgccctacc acacgctagg
    10381 catcaacaaa tatcacttac ttaatctgcc ctatgacgcc ccggaaaaac cgcttgatgc
    10441 gccagaactg ctcgactttg cccagcagta tgcctgccag aaagggttaa ccgcgacctt
    10501 acgaggataa caaccatgac cacactgaaa ctggacacgc tcagcgaccg cattaaagcg
    10561 cacaaaaatg cgctggtgca tattgtgaaa ccgccagtct gtaccgagcg cgcgcagcac
    10621 tataccgaga tgtatcaaca acatctcgat aagccgatcc cggtacgtcg cgcgctggca
    10681 ctggcgcatc acctggcgaa tcgcaccatc tggatcaaac acgatgagtt gatcattggc
    10741 aaccaggcaa gcgaagttcg cgccgcgccg atcttcccgg aatatactgt ctcgtggatc
    10801 gaaaaagaga ttgatgatct ggcagatcgt cccggtgctg gctttgcggt gagcgaagag
    10861 aacaaacgcg ttctgcatga agtgtgcccg tggtggcgcg gtcagaccgt acaggatcgc
    10921 tgctacggca tgtttaccga tgagcaaaaa ggtctgctgg cgaccggaat cattaaagcg
    10981 gaaggcaata tgacctccgg cgatgcgcac ctggcggtga atttcccgct gctgctggaa
    11041 aaaggtcttg atggtctgcg cgagaaagta gcggaacgtc gctcgcgcat caatctgacg
    11101 gtactggaag atttgcacgg cgaacagttc ctgaaagcga ttgatatcgt gctggtggca
    11161 gtcagcgaac acattgaacg ttttgcagcc ctggcgcgtg aaatggccgc gaccgaaacc
    11221 cgcgaaagcc gtcgcgatga actgctggcg atagcagaaa actgcgatct tatcgcccac
    11281 cagccgccgc agactttctg gcaggcgctg caactgtgtt acttcatcca attgatattg
    11341 cagatcgaat ctaacggtca ctcggtatcg tttggtcgta tggaccagta tctctacccg
    11401 tactatcgcc gcgacgttga actcaaccag acactggatc gcgaacacgc catcgaattg
    11461 ctgcatagct gctggctgaa actgctggaa gtgaacaaga tccgctccgg ctcacactca
    11521 aaagcctctg cgggaagtcc tctgtatcag aacgtcacca ttggcggaca aactctggtt
    11581 gatggtcaac caatggacgc ggtgaatcca ctctcttacg ctatcctcga atcctgcggt
    11641 cgcctgcgtt ccactcagcc taacctcagc gtgcgttacc atgcaggaat gagtaatgat
    11701 ttcctcgacg cctgcgtaca ggtgatccgt tgcggcttcg ggatgccggc gttcaacaac
    11761 gacgaaatcg tgatcccgga atttattaaa ctcggtattg aaccgcagga cgcttacgac
    11821 tacgcagcga ttggttgtat cgaaaccgcc gtcggtggca aatggggcta tcgctgtacc
    11881 ggcatgagct ttatcaactt cgcccgcgtg atgctggcgg cgctggaagg cgggcgtgat
    11941 gccaccagcg gcaaagtgtt cctgccacaa gaaaaagcgt tgtcggcagg caactttaac
    12001 aatttcgatg aagtaatgga cgcgtgggat acgcaaatcc gttattacac gcgtaaatca
    12061 atcgaaatcg aatatgtcgt cgacaccatg ctggaagaga acgtgcacga tattctctgc
    12121 tcggcgctgg tggatgactg tattgagcga gcgaaaagta tcaagcaagg cggcgcgaaa
    12181 tatgactggg tttctggcct gcaggttggc attgccaacc tcggcaacag cctggcggca
    12241 gtgaagaaac tggtatttga acagggcgcg attggtcagc aacagctggc tgccgcactg
    12301 gcggatgact tcgatggcct gactcatgag caactgcgtc agcggctgat taacggtgcg
    12361 ccgaagtacg gcaacgacga tgataccgtc gatacgctgc tggctcgcgc ttatcagacc
    12421 tatatcgacg aactgaaaca gtaccataac ccgcgctacg gtcgtggtcc ggttggcggc
    12481 aactattacg cgggtacctc gtctatctcc gctaacgtac cgtttggcgc gcaaactatg
    12541 gcaacaccgg acgggcgtaa agcccacacc ccgctggcag aaggcgcaag cccggcctcc
    12601 ggtactgacc atcttggccc tactgcggtc attggctcag tgggtaaact gcctacggca
    12661 gcgattctcg gcggcgtgtt gctcaaccag aaactgaatc cggcaacgct ggagaacgaa
    12721 tctgacaagc agaaactgat gatcctgctg cgtaccttct ttgaagtgca taaaggctgg
    12781 catattcagt acaacatcgt ttcccgcgaa acgctgctgg aggcgaaaaa acatccggat
    12841 cagtaccgcg atctggtagt gcgtgtcgcg ggctattccg ccttcttcac cgcgctctct
    12901 ccagacgcac aggacgatat catcgcccgt actgaacata tgctgtaatc tcctcggccc
    12961 ggcggcgtcg tcgccgggct aaatcattca catcatcaat ttcatcctta ctttcattcg
    13021 aaatataatt tgtgctctgc gtcacattgt tattacactg ttacggtttc attttcgagc
    13081 caggagcaca cctatgagcg taaaagttat cgtcacagac atggacggta cttttcttaa
    13141 cgacgccaaa acgtacaacc aaccacgttt tatggcgcaa tatcaggaac tgaaaaagcg
    13201 cggcattaag ttcgttgtcg ccagcggtaa tcagtattac cagcttattt cgttctttcc
    13261 tgagctaaag gatgagatct cttttgtcgc ggaaaacggc gcactggttt acgaacatgg
    13321 caagcaactg ttccacggcg aactgacccg acatgaatcg cggattgtta ttggcgagtt
    13381 gctaaaagat aagcaactca attttgtcgc ctgcggtctg caaagtgcat atgtcagcga
    13441 aaatgccccc gaagcatttg tcgcactgat ggcaaaacac taccatcgcc tgaaagctgt
    13501 aaaagattat caggagattg acgacgtact gttcaagttt tcgctcaacc tgccagatga
    13561 acaaatcccg ttagtgatcg acaaactgca catagcgctc gatggcatta tgaaacccgt
    13621 caccagtggt tttggcttta tcgacctgat tattcccggt ctacataaag caaacggtat
    13681 ttcgcggtta ctgaaacgct gggatctgtc accgcaaaat gtggtagcga ttggcgacag
    13741 cggtaacgat gcggagatgc tgaaaatggc gcgttattcc tttgcgatgg gcaatgctgc
    13801 ggaaaacatt aaacaaatcg cccgttacgc taccgatgat aataatcatg aaggcgcgct
    13861 gaatgtgatt caggcggtgc tggataacac atcccctttt aacagctgac ctctctaccg
    13921 gagccgtgcg ctccggtttt cccttctttt tacatcctgt acatcacatc aaatcattaa
    13981 acttgcatta acttatttat aacttatatt gtcgcatgca aatcagaatg gtttcgaatg
    14041 aaagttaatg agaggtggtt atggcttcta cttttaccag cgacacattg cctgccgatc
    14101 acaaagcagc tatccgtcag atgaagcacg cgctgcgggc gcagcttggc gacgtccagc
    14161 agatctttaa tcagctaagc gatgacattg ccacgcgagt ggctgaaatc aacgcactca
    14221 aagcacaggg cgatgccgtc tggccggtgc tgtcttatgc cgatatcaaa gcaggtcatg
    14281 ttactgcaga gcagcgcgaa cagattaaac gtcgcggttg tgcggtgata aaaggccatt
    14341 tcccccgcga acaagcgcta ggctgggatc agtcgatgct ggactatctg gaccgcaacc
    14401 gctttgacga ggtctacaaa ggccccggcg ataatttctt cgggacgctc agcgcttcac
    14461 gtcccgagat ttaccccatc tactggtcgc aggcgcaaat gcaggcccgc cagagtgaag
    14521 aaatggcgaa tgcgcagtcg tttctcaatc gtctgtggac atttgaaagt gatggaaagc
    14581 aatggtttaa cccggatgtg agcgtcatct accctgaccg tatccgccgc cgtccgcccg
    14641 gaacgacctc caaaggtctt ggagcgcata ccgactccgg ggcgctggaa cgctggctgc
    14701 ttccagcgta tcagcgcgtt ttcgccaacg tctttaatgg caatctggcg caatatgatc
    14761 cctggcatgc ggcacatcgt acggaagttg aagagtacac ggtggacaac accaccaaat
    14821 gttccgtgtt tcggacattc caaggctgga cagcgctctc taatatgctg cctggtcagg
    14881 ggctgctgca cgttgtgccc attcctgaag ccatggcgta cgtactgtta cgtccgctgc
    14941 ttgatgatgt gccggaggat gaactgtgcg gcgtagcgcc cggaagagtg ttgccggtat
    15001 cagagcaatg gcatccactg ttaattgagg cgttaaccag cattccaaaa ctcgaggccg
    15061 gagactccgt ctggtggcac tgcgacgtca tccattccgt tgcccccgtt gaaaatcaac
    15121 agggttgggg caacgtgatg tacattcctg cggcaccaat gtgcgagaaa aatcttgcct
    15181 acgcgcacaa ggtgaaggcc gcactggaaa aaggcgcatc gccgggcgac ttcccgcgcg
    15241 aggactatga aacaaactgg gaaggacgct ttacgcttgc cgacctcaac attcacggta
    15301 agcgagcgtt gggaatcgac agctgagtgt cgtacagccc ttcccgttcc acggcggtgt
    15361 tcccttttcc tgggcgaacc cgccgcactg actcccgcac cgccaatcgt ccgtgcagca
    15421 gcagcgagcc caccggagca tccgggcgaa tcagccgctg ttgcaacatt tgcaccgctt
    15481 cattgcctag ctcatcgcgt ggcacatgca cagccgtcag gggaacatcc tcgattgccg
    15541 caaggttaaa gccgtcgata ctcatcaccg agacatcctg tggtacgcgc agaccacgac
    15601 gatgtagcgc atttaccgtt cccgccgcca taaaatcccc gccgaccaga aacgccgtcg
    15661 gcaacgtctt gccattggcg gcatccagcc acgctccgac cttttcttcc gcttctttag
    15721 cgctaaaact gctgatattc agcaaatgac ggtcatggtg aaaggccaga ttatgctgac
    15781 gccatgattc tttaatccct gcaaaccgct gctccatagt gtagcggcgc aggcacatca
    15841 cattcatcac ctcccggtga cccatttcaa acagatagcg ggcggcgaac gcgccaatta
    15901 accgatgatc cggcgcaatc gccgggaggc gcattcgttc atcacggcag ttgatcagca
    15961 cacaaggctt agcgagatcg gcggccagat catggatgtg cggatcgtca atccccagca
    16021 ggatcgctgc ctgcgtatct gcttcattca tccgcgagag aaagagcgcg ggatcgctgt
    16081 caaactcctc cagcgcgcag taacgcagcc gcacttcatg cggcgataac gctttgctga
    16141 tgctctggat cacacggtaa taaaagatat cagatcgttg gtcaaaagcg cgttgcggcg
    16201 cgaaaataat caggttgttc agcaacatac gacctgctgc catgccatcc atcacgccca
    16261 actcacgcgc gcaggccagc acctgttcgc gagcgcgtgc actggtattc gctttgccag
    16321 ccagcacccg cgaaaccgta ctcggcgata gcccggtacg tgcggcgata tcggcgattt
    16381 tcagcttctt gtccattttg tgatctgcct ctaattcgga aatgacagaa ttttcatgag
    16441 caaaaaagcc cattctgtct ggcattaaat ccatttcagt aatgtatatc agtcactcat
    16501 ggaaaaacgt gcacaaaacc cgctttcttt cgcttttagg gtctcttact gtgaattggt
    16561 agaacaactt ccatacaaaa ataatggaaa aacgaacaat aaagacttta tttatcaaaa
    16621 agttaaaagt ggtgtaacaa ctccattctg acgttgccgc acaaacccta taccattctg
    16681 tggagtgcat atgagtcagg gtatcaataa cgatatgact gccagtaaac cgcgccatat
    16741 cgtcaaaaat ctgcgttggt ggatactggt cctttttttg ctcggtgtta ctgtcaacta
    16801 catcacgcgt aactcgctgg gtattctcgc ccccgaactg aaaagcagcc tagggattac
    16861 caccgaacaa tattcctgga ttgtaggtgc tttccagctc gcctataccc tattccaacc
    16921 gttgtgcgga tggctgattg acgtcattgg cctcaaactg ggctttatga tctgcgcggg
    16981 actgtgggca ctggcctgtc tgatgcatgc gggggcggga aactggggac atctggcgct
    17041 gttgcgcttc tttatgggcg gtgcggaagc cgcggcaacc ccagcaaacg ccaaaaccat
    17101 cggtgaatgg tttccaaaat cagaacgccc gatcgccgca ggctgggcag gcgtcggttt
    17161 ttccatcggt gccatgctgg ccccaccgat tatctatttt gcccacgctt cattcggctg
    17221 gcagggtgca tttatgttca ctggcatcct ggcgatgctg tgggtggtgc tgtggtgggc
    17281 gttctatcac agcccggata agcatcctaa cctcagcaaa gccgagctgg actttattcg
    17341 ccaggataac gaagcgcccc cggtcaagtt accctttctg accgcactga aaaccgtggg
    17401 caaaaataaa cgcttttacg gtatcgctat cccggccttt atggcagaac cggcgtgggc
    17461 ggtgatgagt ttctgggtgc ctctatacct gtccaaaacc tatggcatgg acctgaagca
    17521 gatcgcatta ttcgcctggt tgccgttcct cgccgctgat cttggcagta ttgccagcgg
    17581 ctatctgacc aaactttaca cccgcctgtt tgggctgagt cgtgtgaact ccgttgtcgc
    17641 atcatcggtc agtggcgcat tcttgatggt ttcgctggcg gtgatggcct ttaccaataa
    17701 cccgtatatc gccatcgtcc ttatctccat cggcggcttc ggtcaccaga tcatctcctg
    17761 catgctgagc gcactggtgg tggaatcctt cgacaaaggt cagatggcca ctgttaacgg
    17821 catgcgcggt tctgccgcgt ggatcgcaag tttcctgttc tccctgatta tcggggttac
    17881 cgctgacaaa atcggcttta accccctgtt tatcgccatg ggcttctttg acctgattgg
    17941 cgctttcttc ctggtcacct ttattgctga acgtcgcgca aaacgcgcct gagagtggat
    18001 gtaaacatga aaacgctgaa aaactggacc ctgcacaaac agtctgccca ccacgttgaa
    18061 ctgctggtcg atggccagca tatcctgtgc ctgtacgtgc tggaagaaaa tctgttccgc
    18121 gtgctgttaa aacgcaaagg cgagctggcg ctagatcgca cctggagcat tgctccggcg
    18181 caggatgtgc catgggaagg gcgcagccgc gaagatctca gcggctttag cctgcccgcg
    18241 tggacagtga tccagcacga cgacaccgtg gttatcgcca ccgagtcact gcgtgcaacc
    18301 gttcaccagc cgctgtggct ggagtggcac tatcgtaaca acgaaggcga atggctgccg
    18361 ttggtgaatg accgcccgac cagcgcttat ctgctgaatg cccacggcga tggcgtggca
    18421 cactatcaga gccgtcgtaa agatgaacgc ttttatggcc tcggagataa agccggcgat
    18481 ctgcaacgca acggtaaacg ttacgagatg cgtaacctcg acgcgatggg ctacaacgcc
    18541 gttagtactg acccgctgta caaacacatt ccattcacca tcacccgccg cgatgacatt
    18601 agcttcggcc tgttttatga caacctcagc agctgctggc tggatctggg caacgagatc
    18661 gacaactacc acaccgccta tcgccgctgg caggcggaag cgggtgatat cgactactac
    18721 ctgtttaccg gcgaacgcgt acttgatatc accaaagcct tcgtacgcct gaccggaaaa
    18781 accctgttcg gaccgaaatg gagcctgggc tacagcggtt caactatgca ctacaccgat
    18841 gcgccggacg cacaaaatca gttgatgaac tttatccgcc tgtgtgaaga acatgcaatt
    18901 ccctgcgatt cgttccagct ctcttccggt tatacctcaa ttaacggcaa acgctatgtg
    18961 ttcaactgga actacgacaa agtgccgcag ccgaaagtga tgagccaggc atttcacaat
    19021 gctggtttgc acctggccgc caatatcaag ccgtgcctgt tgcaggatca cccacgctat
    19081 aacgaagtgg cagaaagagg actgttcatc cgtgattcag aaacagatga accagaacgt
    19141 tccagcttct gggatgacga aggttctaac ctcgagttta ctaatccgca gaccatccag
    19201 tggtggcagg aaggcgtgac cacacagctt ctggagatgg gtatcgactc cacctggaac
    19261 gacaacaacg aattcgaagt atgggacggt gaagcacgct gtcacggctt tggtcagacg
    19321 atcgcgatca aacatattcg cccagtgatg ccactgctga tgatgcgagc ctcgatggaa
    19381 gcccagcagc gtttcgctcc ggaaaaacgt ccgtacttga tctcccgctc cggctgcgcg
    19441 ggaatgcagc gctacgtgca gacctggagt ggcgataacc gcactagctg ggacactctg
    19501 cgctacaaca cccgcatggg tctggggatg agcctctccg ggctgtataa cctcggtcac
    19561 gacgtcggcg gtttctccgg tgataagccg gacccggaac tgtttgtacg ctgggtacaa
    19621 aacggcgtga tgcatccgcg ctttacgatt cactcatgga acgatgacca taccgtaaac
    19681 gaaccgtgga tgtatccggg cgtcactccg gcgatccgca gcgctattga gttgcgctat
    19741 cgcctgctgc cctactttta taccctgatg tggcaggcat acgccgatga tgaaccaatg
    19801 ctacgtccga ctttccttga ccacgagcac gacgttcaga cttttgaaga gtgtgatgac
    19861 ttcctgcttg gtcgcgatat tttggttgcc agcgtcgtag agcaaggcga gcgccagcgc
    19921 cgtgtatggc tgccagataa cgaaactggc tggtacgatt tctacaacgg cgaatggttc
    19981 agtggcggtc agtggatcac cctcgacgca ccgctggaga aactgccgct gatggttcgt
    20041 gccggggctg gtctaccgct cagcaaacgc atcacctatg tgagcgcaga acaagacgat
    20101 acccgcgaac tgaaactgtt cccgctgaaa ggtgtgggca caacgtccgg cctgctgttt
    20161 gaagacgatg gcgaaagctg gggctaccaa actggtaatg cgctttgggt ggaatgggaa
    20221 atggtttgcg atggggcgac catcaacctg aaagtcaacg cgcgcgggga ttatcgtcct
    20281 gcatggagtg cactgaaagt gtcattacca ttggaagaaa aacgcacgct gctggtcaat
    20341 ggtgttgaag ggagtgaatg gatgcgttaa atcattaccg gggccgggag gcgactgcac
    20401 cttacccggt ccagccatcg aatcctttac ctcagtattt cacgctgcag gaaggcagca
    20461 aacctgtaaa ccccgggagc ttaaataagt aagtgaccgg ggtgaacagg tgaagccaat
    20521 gcatctgccg catgaaagac ggcggggaat taagcgtcga tccctttact acgcaggtaa
    20581 tcttcgtaat taccgctaaa gtcgatcgcg cgttccgggg tgatttccag aatgcgggtc
    20641 gccagggagc ttacgaactc acggtcgtgt gaaacaaaga tcagcgtgcc ctgatacagt
    20701 tccagcgcca tgttcagtga ctcgatggat tccatatcca ggtggttggt cggttcgtcc
    20761 atgatcagaa tgttcggctt ctgcatcatt aacttaccaa acagcatccg ccctttttcc
    20821 ccaccggaaa gcactttagc tggctttttg atgtcgtcct ggctgaacag caaacgaccg
    20881 agaatgctgc gtaccgcctg ctcgtcatcg ccttcctgct tccactgact catccattcg
    20941 aacacggtca gatcattttc aaactcatat tcgtggtcct gggcgtagta accaatgcgc
    21001 gcgttttcag accattttac ggtgccgctg tccggttgca gatcgcccac cagcgttttc
    21061 agcagcgttg atttaccgac gccgttggta cccagtaccg caagtttttc accgacctcc
    21121 agcagcaggt tgagattttt aaacagtgga ccgttatcaa accctttggt cagaccttcc
    21181 acttccagcg cgttacggaa cagtttctta tcctgttcaa aacggatgaa cgggttctga
    21241 cggctggagg ctttcacctc ttccagtttg attttatcaa tctggcgcgc gcgcgaagtt
    21301 gcctggcgag atttcgaggc gttggcgcta aagcggctaa cgaaagattg caactcagca
    21361 atctgcgctt tcttcttggc gttatcggcc agcagacgtt cacgcgcctg ggtcgccgcc
    21421 gtcatgtact catcgtagtt acccggataa acgcgcagct cgccgtaatc cagatccgcc
    21481 atgtgggtac agaccatgtt aaggaagtga cggtcgtgcg agatgatgat catggtgctg
    21541 tcacgctcgt tcagcacctg ttccagccag cgaatggtgt cgatgtcgag gttgttggtc
    21601 ggttcgtcga gcaggagaat ctccggatca gcaaacagcg cctgcgccag aagcacacgc
    21661 agtttccagc caggagcaac ttcactcatc gggccgtagt gctgttccac tggaattccc
    21721 acgccaagca acagttcacc ggcgcgagct tccgcagagt aaccgtccat ttcgccgtat
    21781 ttaacttcca gatcggccac tttatagccg tcttcctcac tcatttccgg caaagcatag
    21841 atgcggtcac gctcctgctt cacttcccac aactctttat gccccatgat caccgtatcc
    21901 agcacagtga actcttcaaa ggcaaactga tcctgacgca gcttaccaat gcgctcgttg
    21961 ggatcgaggg aaacgttacc caaagtcggc tctaagtcgc cgccgaggat cttcataaag
    22021 gtggatttac cactaccgtt cgcgccaatc aggccgtaac ggttgccgcc gccgaatttg
    22081 acggaaatgt tttcaaacaa cggcttactg ccgaactgca tggtgacgtt actggaaact
    22141 aacacaggcg tatcctgaaa agagatatga caaaccgcgc attatgccat aagtctgaaa
    22201 gaagatcctg ctcctcgctt aaggtctaca ctcaagccaa aggcaaaaaa tgtgatttcg
    22261 tacacatctg atttcactgt gagctggaat gaacttataa tgcgcttcca atactctaat
    22321 attctcaacc caatggcctg ccaggcacaa aatctcgctt aacatgaata tgaaattgaa
    22381 aacattattc gcagcggcct tcgctgttgt cggcttttgc agtaccgcct ctgcggtaac
    22441 ttatcctctg ccaaccgacg ggagtcgcct ggttggtcag aatcaggtga tcaccattcc
    22501 tgaaggtaac actcagccgc tggagtattt tgccgcggag taccagatgg ggctttccaa
    22561 tatgatggaa gcgaacccgg gtgtggatac cttcctgccg aaaggcggta ctgtactgaa
    22621 cattccgcag cagctgatcc tgccggatac cgttcatgaa ggcatcgtca ttaacagtgc
    22681 agagatgcgt ctgtattact atccgaaagg gaccaacacc gttatcgtgc tgccgatcgg
    22741 cattggtcag ttaggcaaag atacgcctat caactggacc accaaagttg agcgtaagaa
    22801 agcaggcccg acctggacgc cgaccgccaa aatgcacgca gagtaccgcg ctgcgggcga
    22861 accgcttccg gctgtcgttc cggcaggtcc ggataacccg atggggctgt atgcactcta
    22921 catcggtcgc ctgtacgcta tccatggcac caacgccaac ttcggtatcg gcctgcgtgt
    22981 aagtcatggt tgtgtgcgtc tgcgtaacga agacatcaaa ttcctgttcg agaaagtacc
    23041 ggtcggtacc cgcgtacagt ttattgatga gccggtaaaa gcgaccaccg agccagacgg
    23101 cagccgttat attgaagtcc ataacccgct gtctaccacc gaagcccagt ttgaaggtca
    23161 ggaaattgtg ccaattaccc tgacgaagag cgtgcaaaca gtgaccggtc agccagatgt
    23221 tgaccaggtt gttcttgatg aagcgattaa aaaccgctcc gggatgccgg ttcgtctgaa
    23281 ttaatcttca aaacttaaag caaaaggcgg actcataatc cgcctttttt atttgccaga
    23341 ccctagttgg ccgggagtat aactaacaaa acatatccca ccaacgccgc ccacaacagc
    23401 atcggtattg aatagagatg gaaacgccac cagatgcggc gatcgttcgc catacgtagc
    23461 gcaatcaaat ttgccagcga tccgggtaat aacccaaagc cacctacgtt taccgcccat
    23521 accagtaata aagacggcgg cacatagttc agcaacaata tggtactcgg cacgttactg
    23581 atcacctgcg ataaaccgat tgccgttaac cataacccgg gttcagatag atgactcacg
    23641 ttacccaaca cgccttgcaa cgctggaagc tgggtcagta aatggacgtc gataaacatc
    23701 gccataaaca ccagcagcag cgtccagtcc acactgagca ccacgcgacg tgccagcagc
    23761 gcaaagcctg ccgccacaat caccagtccc cacagctctt gtttgaactc cagcgccgtc
    23821 agaaagacga tatacagccc cagacaactc cacaccagcc gcggtttcca ctccggtgtt
    23881 tgcacccccg tatggtattg cagcgccttt ccagggaaac aacaccagca caggagcagg
    23941 agcgtcagca tcattgcgcc agccagcggt gccatttggg caataaatcc ggcaaacgaa
    24001 agaccagaac gtccccagat aagaatattt tgcgggttgc caattggcgt cagtagcgaa
    24061 ccagcgttga ctgccagcgc ctcaaaaata atcagccgat taaccgggat ctcacacagt
    24121 ctttttagcg tgatagtcag cggaacaaca ataaacagcg cgacatcgtt ggtcagaaag
    24181 gtagaaagca gcgccgccgc cagcaccata aacatcgcca gccgacgctc cgtagcaaag
    24241 cggcgcacca ttttgcgccc cagcacatca aaataaccgc ttaactccac acctttggtc
    24301 agcagcatca ggccgcttaa ggtgatgatg gtgtgccagt cgatagcagc aggccaggat
    24361 ttcggtgcaa agggcacgaa aaagcttaat ccgataccaa caagaattaa taactgaaaa
    24421 aaacgatcgc cttgcagcgt gcgtaaaaaa ggcaggctca tttggcaccg tgtttctggg
    24481 taaacaaacg aaaagcgtcg agcgtctctt cactaacatg gtgctccatg ccttccgcgt
    24541 cgcgacgggc gatttccgga ctgacgccca acaccaacaa gaaattttcg actatctgat
    24601 gtcgctcgcg gctttcctgc gccagcttct ctccttctgc cgttaaaaac acgccacgcc
    24661 aggggatcat ttcaatcagc cccatggttg ccagccgctt aagcatttta gccaccgtcg
    24721 gttgcgaaac tcccagacga gcagccatgt ccacctgacg agcttccccc acttccctga
    24781 tcaagtcaga aatcagctca acgtaatcat caataagctc gcgccgatgc gcctctctga
    24841 cctggcggaa cccttcaacg tgctcttcca cgttcactaa ctgcgtcact ttttttgctg
    24901 ttggcgtacc tgcgcgacga ctcattgtgc ttcctcatta tggtgacgca tacatagcgt
    24961 tctcatttcg tgcgcacatt gtaaaccaga gttgcgaagg tacaaaaaat taacgtttta
    25021 gcaatagcta cataatatag cctgtgctat atctgtatgt aatgcaatca tccctcaagg
    25081 atcgacggga ttagcaagtc aggaggtctt atgaatgagt tcaagaagtg tatgcgcgtg
    25141 tttagtcatt ctccctttaa agtacggtta atgctgctct ctatgttgtg cgatatggtc
    25201 aacaacaaac cgcagcaaga taaaccttcc gataaatagc ggcgtcgcgg tacgccgctt
    25261 cactcctgct tttatgcagg cataacgcgt tttggtctga aaaaccccac tttttgtcgg
    25321 atttgcaatc cccttcgcaa aagatttgtt cgtcagtagt tgacctgaac ggcggctcgc
    25381 tctatcttct tgcagcccta cgtatattgc ggctcgcgga tgcggacccc tttcccctct
    25441 tcacgcactc ttgcaggtat tgacccttga cgccagggta agcacatggc gtttgttacg
    25501 atagtggcat atgaatttaa ccctcaaaga atcgcttgtt acccgtagcc gggtatttag
    25561 cccgtggact gcgttctact ttttacagtc gctattaatt aacctcggct taggttaccc
    25621 cttcagtttg ctctacaccg ctgcgtttac ggctattttg cttttgctat ggcgaacatt
    25681 gcctcgcgta caaaaagttc tggtcggtgt cagttcgctg gtggcggctt gttatttccc
    25741 ttttgctcag gcctacggcg cgcctaattt caatacattg ctggcattgc actccaccaa
    25801 tatggaagag tcgaccgaaa tcctgacgat ttttccgtgg tacagctacc tggtcggctt
    25861 atttattttt gcgctcggcg taatagcaat caggcgaaaa aaagagaatg aaaaagcgcg
    25921 ctggaatacc ttcgacagcc tgtgtctggt attcagtgtg gcgacatttt ttgttgctcc
    25981 cgtgcaaaac ctggcctggg gtggcgtatt taaactgaaa gatactggct atccggtatt
    26041 tcgttttgct aaggatgtca tcgtcaataa taacgaggtg attgaagagc aagaacggat
    26101 ggcaaaactt tccggaatga aagatacctg gacggtcact gccgttaagc cgaagtatca
    26161 gacctatgtg gtggtgatcg gcgaaagcgc gcgtcgcgat gccctcggtg cctttggcgg
    26221 tcactgggac aataccccgt ttgccagcag cgttaacggt ttgatatttg ctgactacat
    26281 tgccgccagt ggctccacgc agaaatcact tggcttaacg ctcaatcgcg tagtcgatgg
    26341 caaaccacag tttcaggata actttgtcac cctggcaaat cgcgcgggct tccagacctg
    26401 gtggttttcc aaccagggcc aaatcggcga atacgatact gctatcgcca gcatcgccaa
    26461 acgtgcagat gaagtgtact tcctgaaaga aggtaatttc gaagcagata aaaacacgaa
    26521 agacgaagcg ttactggata tgaccgctca agtgctggcg caagagcact cgcaaccgca
    26581 gctgattgtt ctgcatctga tgggctcgca tccgcaggcc tgcgacagga cacaaggcaa
    26641 atacgaaacc tttgtgcaat cgaaagaaac gtcgtgctat ctctatacca tgacgcaaac
    26701 ggacgattta ctgcgcaagc tgtacgatca gttacgcaac agcggcagca gcttctctct
    26761 ggtttacttt tctgaccacg gtctggcctt taaagagcgc ggcaaagacg tgcaatacct
    26821 tgcccatgat gataaatatc agcaaaattt ccaggtgcct tttatggtca tttccagcga
    26881 cgataaagcg catcgggtga ttaaagcccg ccgctcagcc aatgacttct taggcttttt
    26941 ctcgcagtgg acgggaatta aagcgaagga aataaatatc aaatacccgt ttatatctga
    27001 gaagaaagcc gggccgatat acatcaccaa cttccagtta cagaaggtgg attacaacca
    27061 tctcggaacc gatattttcg acccgaaacc ttaaaacaaa aaatccgccc cgagaggcgg
    27121 attttttata tcaccaaagt gattagaagc ggtaaccaac accggcaatc caggtgccta
    27181 cgtcaacgct acgaatacgg ctctgctcgt aagagaagtc cagagcaacg ttttccatcg
    27241 ggttgaactg cagaccagca ccgtaggaga aaccgtagtc gctggtgtcg tgtttgtagg
    27301 tcgggtattc agtggtctgg aatttaccat aacccacccc cactacaccg tagatgcttg
    27361 cccagtcgtt aatgcggtaa gccggaccag cagtgatgcc gtagtactgg tttttgttgt
    27421 agtcaccaga gcttgcagta cggcttttct cggtgtaagt gaaagaaccg atcacaccca
    27481 gcgggctgtt gtcttcttca tagcggtatt tcaggttgaa accgcccatt ttgttcattt
    27541 ggccctgagc gtcgctctgt gcgtaaccgc cagttacggt agaagtcgca gctacggaag
    27601 tacctgcggt gaaagccaga actgcggcca gtgctgaaag acatgcaatt tttttcataa
    27661 ccacctcaaa tgtgattcaa ataagtccta agttttaaat atatcaaaaa ttaatgggaa
    27721 actcttcgcg atttgtgatg tctaacgggc catttcatgt aacagaacgt ttccatacac
    27781 cgctatccat ctaaatttaa atcacttttt cagagaactg cgtaagtatt acgcatgttt
    27841 tccctgtcat tcatccagat tattcctaat caccagacta atgattccat caatcctggc
    27901 gcattttagt caaaacgggg gaaaattttt tcaacaaatg ctcaaccagc attgggtata
    27961 tccagtacac tccacgcttt acttaagtct agatatttgt gggagaaagg atgcctggtt
    28021 cattacgtaa aatgccggtc tggttaccaa tagtcatatt gctcgttgcc atggcgtcta
    28081 ttcagggtgg agcctcgtta gctaagtcac tttttcctct ggtgggcgca ccgggtgtca
    28141 ctgcgctgcg tctggcatta ggaacgctga tcctcatcgc gttctttaag ccatggcgac
    28201 tgcgctttgc caaagagcaa cggttaccgc tgttgtttta cggcgtttcg ctgggtggga
    28261 tgaattatct tttttatctt tctattcaga cagtaccgct gggtattgcg gtggcgctgg
    28321 agttcaccgg accactggcg gtggcgctgt tctcttctcg tcgcccggta gatttcgtct
    28381 gggttgtgct ggcggttctt ggtctgtggt tcctgctacc gctggggcaa gacgtttccc
    28441 atgtcgattt aaccggctgt gcgctggcac tgggggccgg ggcttgttgg gctatttaca
    28501 ttttaagtgg gcaacgcgca ggagcggaac atggccctgc gacggtggca attggttcgt
    28561 tgattgcagc gttaattttc gtgccaattg gagcgcttca ggctggtgaa gcactctggc
    28621 actggtcggt tattccattg ggtctggctg tcgctattct ctcgaccgct ctgccttatt
    28681 cgctggaaat gattgccctc acccgtttgc caacacggac atttggtacg ctgatgagca
    28741 tggaaccggc gctggctgcc gtttccggga tgattttcct cggagaaaca ctgacaccca
    28801 tacagctgct ggcgctcggc gctatcatcg ccgcttcaat ggggtctacg ctgacagtac
    28861 gcaaagagag caaaataaaa gaattagaca ttaatt
//
LOCUS       DAEEKQ010000034        28702 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.034, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000034 DAEEKQ010000000
VERSION     DAEEKQ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28702
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.034"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(36..119)
                     /locus_tag="KLK57_001488"
                     /pseudo
     CDS             complement(36..119)
                     /locus_tag="KLK57_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 domain-containing protein"
     gene            complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="KLK57_001489"
     CDS             complement(237..1676)
                     /gene="rsmF"
                     /locus_tag="KLK57_001489"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HBE0891890.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAPQRVMDVAAAPGSKTTQISARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGAAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNQEENEAVCLWLKETYPDAVEFLPLGD
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPAPKYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALATPDNVNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="KLK57_001490"
     CDS             complement(1757..4390)
                     /gene="yebT"
                     /locus_tag="KLK57_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HBE0891891.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVNPSVVTL
                     LRENTRIELLNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="KLK57_001491"
     CDS             complement(4359..5642)
                     /gene="yebS"
                     /locus_tag="KLK57_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HBE0891892.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            5772..6269
                     /gene="msrC"
                     /locus_tag="KLK57_001492"
     CDS             5772..6269
                     /gene="msrC"
                     /locus_tag="KLK57_001492"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HBE0891893.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            6366..7064
                     /gene="proQ"
                     /locus_tag="KLK57_001493"
     CDS             6366..7064
                     /gene="proQ"
                     /locus_tag="KLK57_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HBE0891894.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDALRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            7084..9132
                     /gene="prc"
                     /locus_tag="KLK57_001494"
     CDS             7084..9132
                     /gene="prc"
                     /locus_tag="KLK57_001494"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HBE0891895.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMRDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            9324..10205
                     /gene="htpX"
                     /locus_tag="KLK57_001495"
     CDS             9324..10205
                     /gene="htpX"
                     /locus_tag="KLK57_001495"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HBE0891896.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(10251..11314)
                     /locus_tag="KLK57_001496"
                     /pseudo
     CDS             complement(10251..11314)
                     /locus_tag="KLK57_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            11491..12282
                     /gene="kdgR"
                     /locus_tag="KLK57_001497"
     CDS             11491..12282
                     /gene="kdgR"
                     /locus_tag="KLK57_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HBE0891897.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDLDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            complement(12425..12664)
                     /locus_tag="KLK57_001498"
     CDS             complement(12425..12664)
                     /locus_tag="KLK57_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891898.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            12823..12966
                     /gene="mgrB"
                     /locus_tag="KLK57_001499"
     CDS             12823..12966
                     /gene="mgrB"
                     /locus_tag="KLK57_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HBE0891899.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            complement(13134..14114)
                     /locus_tag="KLK57_001500"
     CDS             complement(13134..14114)
                     /locus_tag="KLK57_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5-like element ISKpn26 family transposase"
                     /protein_id="HBE0891900.1"
                     /translation="MSHQLTFADSEFSTKRRQTRKEIFLSRMEQILPWQNMTAVIEPF
                     YPKAGNGRRPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLDSALPDR
                     TTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTKNKEQQR
                     DPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHGEEQFVS
                     ADAGYQGAPQREELAEVDVDWLIAERPGKVKTLKQHPRKNKTVINIEYMKASIRARVE
                     HPFRIIKRQFGFVKARYKGLLKNDNQLAMLFTLANLFRVDQMIRQWERSQ"
     gene            14241..14528
                     /locus_tag="KLK57_001501"
     CDS             14241..14528
                     /locus_tag="KLK57_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HBE0891901.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            15198..15341
                     /locus_tag="KLK57_001502"
     CDS             15198..15341
                     /locus_tag="KLK57_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HBE0891902.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            15354..15563
                     /gene="cspE"
                     /locus_tag="KLK57_001503"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             15354..15563
                     /gene="cspE"
                     /locus_tag="KLK57_001503"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBE0891903.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            15729..16538
                     /gene="rlmA"
                     /locus_tag="KLK57_001504"
     CDS             15729..16538
                     /gene="rlmA"
                     /locus_tag="KLK57_001504"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HBE0891904.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLSVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDDKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTFCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFTWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(16535..17101)
                     /gene="mntP"
                     /locus_tag="KLK57_001505"
     CDS             complement(16535..17101)
                     /gene="mntP"
                     /locus_tag="KLK57_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HBE0891905.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
     gene            complement(17530..17988)
                     /locus_tag="KLK57_001506"
     CDS             complement(17530..17988)
                     /locus_tag="KLK57_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HBE0891906.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
     gene            complement(18043..18894)
                     /gene="manZ"
                     /locus_tag="KLK57_001507"
     CDS             complement(18043..18894)
                     /gene="manZ"
                     /locus_tag="KLK57_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HBE0891907.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(18907..19707)
                     /gene="manY"
                     /locus_tag="KLK57_001508"
     CDS             complement(18907..19707)
                     /gene="manY"
                     /locus_tag="KLK57_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HBE0891908.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(19770..20741)
                     /gene="manX"
                     /locus_tag="KLK57_001509"
     CDS             complement(19770..20741)
                     /gene="manX"
                     /locus_tag="KLK57_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HBE0891909.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            21204..22754
                     /gene="yoaE"
                     /locus_tag="KLK57_001510"
     CDS             21204..22754
                     /gene="yoaE"
                     /locus_tag="KLK57_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HBE0891910.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(22758..24356)
                     /locus_tag="KLK57_001511"
     CDS             complement(22758..24356)
                     /locus_tag="KLK57_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBE0891911.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDMLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(24487..25851)
                     /gene="sdaA"
                     /locus_tag="KLK57_001512"
     CDS             complement(24487..25851)
                     /gene="sdaA"
                     /locus_tag="KLK57_001512"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBE0891912.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(26035..26613)
                     /locus_tag="KLK57_001513"
     CDS             complement(26035..26613)
                     /locus_tag="KLK57_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HBE0891913.1"
                     /translation="MEYRSLMLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            complement(26617..27978)
                     /gene="pabB"
                     /locus_tag="KLK57_001514"
     CDS             complement(26617..27978)
                     /gene="pabB"
                     /locus_tag="KLK57_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HBE0891914.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITAQLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            28052..28231
                     /locus_tag="KLK57_001515"
     CDS             28052..28231
                     /locus_tag="KLK57_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HBE0891915.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            complement(28351..28674)
                     /locus_tag="KLK57_001516"
     CDS             complement(28351..28674)
                     /locus_tag="KLK57_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HBE0891916.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMESGERSVIKNPEYFSTYMQEE
                     LKALV"
BASE COUNT         7311 a         7087 c         7275 g         7029 t
ORIGIN      
        1 cataaaacac tatcaataag ttggagtcat tacctggtta acgttcgatc acccggcgat
       61 tcaccgtgta attcgccgtc gtcgatttca aaagcgccta tgcgcacact ggttttcatc
      121 ttcgtgctct ccatttagct gccggttgtg gtacgaccag tccgatcgca ccatttttca
      181 gcctagttga ggcagccgaa tgtgccagtc aaaaagtgcg ctttttttgt gcgctgtcag
      241 gcgttaccgg taaaaagttt cccatcgcgc accagttcac gcggatagct gtttttcaat
      301 cgcgaaccaa tccgtttggc taaaccaatc ggttgatgct ggaaagtaac caacacgtca
      361 tccgccactg gcgcggcttg cgggtaaaca tcgcgcccgc gataccactc ctccgcttcc
      421 tgcggtgtca gttcaaaagc gttcacattg tcgggggtgg caagggcaat aactgcttca
      481 tgctgccagc gataaccttt gttgtgcgtt tcggcaagtt taatccccaa gcgagaaaat
      541 cggactttac cgatcagggc ttcaatgccc accgggaaca accacagttc tttgtcacgc
      601 tgccagaggc gcaggttttc atcccagttt aagccaacac ctgcagccgc ctgacgaatt
      661 tgtccagctt ctcgatcttt cactgggctg aacggaaaat taccgacttt gtatttgggg
      721 gcgggtaagg cgggaatcgc ctgagtttta cgcagacgag caacgaagaa gccttcgcag
      781 tcgtaaattt gtgggaaaac atgcaaaaag ccttcctcgg tcagcgcctt gtttgcacca
      841 gggaagagat caccaagcgg taaaaactct actgcgtcgg ggtaagtctc tttcagccac
      901 aggcaaacgg cttcgttttc ttcctggttt aaggtacagg tcgagtaaac cagcgtaccg
      961 ccagggcgta atgcatgaaa ggcgctgtcg atcagttccc gttgggtagc tgcgatttcc
     1021 tgattgcttt ctggtgacca gttttttagc gcatcgggat ctttacgcac cacgccttcg
     1081 ccggagcagg gagcgtccag caaaatggca tcaaacattt ctggcactgc cgcaccaaac
     1141 acgcggccat caaaatgtgt gagcgcaaca ttactgatac cacagcggct gatattggca
     1201 tgtaacactt ttacccgact ggcggaaaac tcattggcaa gaattgcccc ttcgttattc
     1261 atccgcgcgg aaatttgcgt cgttttggag ccaggcgcgg cagcgacatc catcacccgc
     1321 tgtggtgcat taccgtcagc aaacaaggcg gcgacgggca acattgaact ggcttcctga
     1381 atataaaaca ggccacttaa atgctcggcg gtactaccca atggcaatgc atcttcattg
     1441 tcgcgttcaa tccagaaacc ttcttcacac cacggaattg gcgtaagcgt ccagccataa
     1501 ggagcggtta attgcaggaa atcagcaacg gagattttca gcgtattaac gcgaatgctg
     1561 cggcgcaacg ggcgctgaca ggcggcaaga aaatcatcaa aagagagcgt cgaaggcatc
     1621 gcttcgcgca tttgcgtcag aaaggcgtcc gggaaataaa cggtgtgttg ggccacgagc
     1681 atataccact ggcaaaaaaa acaggcgcgc agtttagcat aaacgctccg gcgcaggcac
     1741 gccggagcag tgggcattat ttgggaagcg cagtacccca ttcacgccac tctttcggtt
     1801 cactttcctg caacaggaag tgcttgcctt cctgggcttt cggtgccagt ggcgtacccg
     1861 gaggcgtggc gaaggcgatg ccgccacgga taaactggtt aaaggtgccg gttttcacta
     1921 cgccgcccgt cagaccaaag tccagactgt aacccgatgc caaccagaag acggaattgt
     1981 tacgcaccag gtgttgatag cgtttactga tgcgcatcgc aatcatcacg cgatctgaca
     2041 atgtccccag cgtcattccg gtaaccgtac ctacttccag accacggaac agcacaggcg
     2101 taccaatacc taacgaaccg gcttccggtg cttcgacaat aatgcttaag ccatccaggt
     2161 aacgcgaatc agtaatagta gcttcttgca actcaaagtc acggcgagga ttaccccgac
     2221 ctggttcgac attgatatac ggctggagga tagtatctag atgctcaacg cccgcggctg
     2281 aaatttgcgg tgtgaccacc gagaagcgcg taccgccgcg ggcgaaggtc tggacatact
     2341 ccggatagag caccgccttt gcctgtactt cattgcgcgc ggtaatcaga tccagcgtct
     2401 ggatttgccc gatatcaata ccgagatagc gaattggcat cccgaccgcc agttttccgg
     2461 catcgaaagc gtgaagcgta atctgcccac caaccgcacg ggccgctgtt tcggaagcat
     2521 acagaattcg tttgtcgcct ttacgctgac tggcgctggc accgctgagg ttatcgaagc
     2581 taatggctcc ctttaatgct ctggagagcg gggatgcctg tacggtcagg ccactaccat
     2641 tcagctgaac tttcgccccg ccttctgccc agaacacgct attgctggtc agaaggttgc
     2701 gatactccgg cttaatatgc agatcgatat caaacgcgtt agctcgcgga cgcacggtaa
     2761 tcacttcacc aacttcaaat ttacggtaca gcactaccga tcctgcctgc acatccggca
     2821 gcgtctctgc actcaaactc acggttgtgg tgggtaaatc gctaaggctg ttctccagcg
     2881 ctttttccag attggcatac agtggatagc tggctttcat ctcaccttta tcgcctggca
     2941 gaatacgtat cccgccgtta atccattctg aggcgctggc accaagaaac tcaacgccat
     3001 ccagccccac cttcacgtcg acacggctgt tgacgacaaa tttgctatcg ccttttacca
     3061 gttcgcgatg ctgaggctcg atggcgacgg taaaggtaac gcctttgctg gtgagtttac
     3121 gatcaataac ctggcctacc tgcacgccgt gaagaatgag cggctgacca gcatcaatac
     3181 cgtaactttc cggcgcggtc agggtcagcg tcagaacatc aggttcatgc agcagtgctt
     3241 tttcgcctgg cacaacaacg aactctttgc gtggctcgcc atcgccgggt accaactcga
     3301 aggttttgcc agtcagcagg gcgctgagat tggcatcgct aagggataat ttcggattaa
     3361 gtaattcgat gcgggtattt tcacgaagca gggtaacgac gctgggatta actgtcattt
     3421 ccccagtgac tttaccacca ggatttaaat ccagtttagt tagctgtccg acttccagtc
     3481 cctgatacat taatggcgtc gagtcggcgg ttaatccggc cccactcggc agttccagtt
     3541 tgattattac gccacgctgg ctatgggcga ggtcttcata cagaccaaag gtatcttccg
     3601 cctcggcagg tttcgactct tcaggtgaat cgaaggcaat cgcaccgtta accagtgccg
     3661 ccagactttc cagtttcacc ttcgcaccac tgatactgac gttggcatca acgccggaaa
     3721 cgttccagaa acggctacct tttttcacta ggtcggtaaa acgccgctcg atcaggacat
     3781 caatcaccac gccttgcttg ttgggattga tggcatagtc gtagactttt cccacaggga
     3841 tcttgcggaa atagaccaat gaaccgctgt tcagcgaacc gagatcgggg gcttgcaggt
     3901 ggatcatcag atcgccattg tccagtcgat atttcggttg ggtatcgagt gcgacaaagt
     3961 gatcctgctc tttaccttta cctggcatca tgccgatata gttaccaccg acgagggcgt
     4021 ccagcccgga gacacctgcc aacgatgctt ttggcgtcac cagccagaac tgagtctctt
     4081 cgcgcagcgc atctttcata tcggacttga tgctgacctt gacttcaatc ttacgaagat
     4141 cgtcgctgag gctgatatcc tgcactgttc cgacttcaac gccctgataa cgaacaggcg
     4201 tacggcccgg gacaatacca tccgccgaca taaagtcgat ggtgacggta ttaccccggt
     4261 cctgataact gtcccaaatc agccaaccgg caatcattag cgcgatgaaa ggcagcagcc
     4321 agaaaggtga gatacggcgt ttatttttaa tctgcgcttc agtcgtcgaa gcgggcgttt
     4381 cctgactcat gtgcatccca aagtaagcgg ctgtccagcc attccacagc aagaatagtc
     4441 aaaattacgg ctgcgccaaa ataaaacgca gccggtccca tagtaaaagc gaggatctga
     4501 tcgcgattaa tcagcgacat ggttaaagat atgacaaaca ggtcgagcat cgaccagcgt
     4561 ccaatccagg tcaccatccg cagtaacaga atgcgtgtgc gtaaaccttg ctggcattta
     4621 aaatgaatgc tcaacagtaa agtgaacatg acgatcactt tagtaaacgg taccagaata
     4681 ctggcgataa acacgattcc ggcgaccgca atgttgctac ttgccagcga cataatcccg
     4741 gaaagaatcg tatcttcctg ccggccacca ttcagataaa tgatagaaat aggcaatagg
     4801 ttggctggta acaacaaaac gattgacgct aacagcgccg cccagcattt ttgcaaacta
     4861 tgacgacgac gcaggcgtag cgggatatgg caacgcgggc agcgaccacg ctgatcagga
     4921 tagccggtaa aatggcaccc aagacagaca cgaagtttct cgtccctacg cgtagcgggg
     4981 cgctgcggat aatatcgctc ccacagctcc tcaacattaa gatgtgacaa cgtcaccgtc
     5041 gttaaaatca ccaacgccac aaaagagaac aagccgacac ccgcctggat atgggcataa
     5101 tcctgtacct ttatagaagc aacgccaatg ccgaccaggt agatatccag cattacccac
     5161 tctttaagac gctcaagcat cagcagtacc ggacgtaaat tcattcccag tcggttacca
     5221 aaccataaat atgctatgga ggacaccaga atgagggggg caccaataac gcagaaaaag
     5281 accatcgccc ccgttatcgt atcgccctgt ttggtcattt gccagatgcc ttgcatgacg
     5341 ttggcgtcga tacgaatacc taacagccag atatgcaaca gcggttcgcc ccaggcaaac
     5401 ggcatcagca acagcatggt gaaagccatt gccgccaggc gcgttagcga ccagtcgcgc
     5461 ccgtcgcgaa tttttgcctg acagcgcgga caataggcac tctgatgaga atttatctcg
     5521 ggcaggctaa acagcatgtc acattgcggg caacgttggt aatcaccacg cggcagttcc
     5581 tcgccgattg ccctcactgt tatctttttt gtcggcgtaa tttgtggtgt gttaagagcc
     5641 atgacgtgcc aaatgtaata agaattaaca ttaatcttaa cccatgatgg gaatgatctt
     5701 gagcattaac gcatttaatg cttattttaa taggctggca gttccaggta agacaaacaa
     5761 gtgattatat aatgaacaaa acagaatttt acgcggattt aaatcgcgac tttaacgcgc
     5821 tgatggcggg agaaaccagt tttctggcaa cgcttgcgaa caccagtgcg ttgttatacg
     5881 agcgtctcac tgacgtaaac tgggcaggtt tttatttgct tgaggacgat acactggtac
     5941 tcggaccatt tcagggcaaa attgcctgtg tccggatacc cgtcgggcgc ggggtgtgcg
     6001 gcactgcggt tgcccgcaat caagtgcagc gtatcgagga tgttcacgcg tttgacgggc
     6061 atattgcctg tgatgcggcg agtaattctg aaattgttct gccgctggtg gtgaaaaatc
     6121 agattattgg tgttcttgac atcgatagta ccgtcttcgg tcgctttaca gacgaggacg
     6181 agcaaggctt acgtcagctt gtggcacagc ttgaaaaagt gcttgcaacg acggattaca
     6241 aaaaattctt tgcgagcgtc gcaggataat caacggataa cgtagcattt actggtagcg
     6301 tcattataat gacgcctgtt catgcctgcg cttgttggct acgtccgttg taatcaggaa
     6361 atttcatgga aaatcaacct aagttgaata gcagtaaaga agtaatcgcg tttctggccg
     6421 aacgttttcc ccactgtttc agtgcggaag gtgaagcgcg tccgctgaaa atcggtattt
     6481 ttcaggattt ggtcgatcgt gttgccgggg aaatgaacct gagcaaaacg caattacgat
     6541 ccgctttacg tctctacact tcgagctggc gttatcttta cggtgttaaa cccggcgcaa
     6601 cgcgtgtcga tcttgacggc aacccatgcg gtgagctgga cgagcaacat gtagagcatg
     6661 ctcgcaagca gcttgaagaa gcgaaagcgc gtgttcaggc acagcgtgct gaacagcaag
     6721 cgaaaaaacg cgaagctgcc gcagctgctg gtgagaaaga agacgcactg cgccgcgaac
     6781 gcaagccacg tccgaccacg ccacgccgca aagaaggcgc tgaacgtaaa cctcgtgcgc
     6841 aaaagccggt agagaaagcg ccaaaaacag taaaagcacc tcgcgaagaa cagcacaccc
     6901 cggtttctga catttcagct ctgactgtcg gacaagccct gaaggtgaaa gcgggtcaaa
     6961 acgcgatgga tgccaccgta ttagaaatca ccaaagatgg cgtccgcgtc cagctgaatt
     7021 cgggtatgtc tttgattgtg cgcgcagaac acctggtgtt ctgaaacgga ggccgggcca
     7081 ggcatgaaca tgttttttag gcttaccgcg ttagctggcc tgcttgcaat agcaggccag
     7141 accttcgctg tagaagatat cacgcgtgct gatcaaattc cggtattaaa ggaagagacg
     7201 cagcatgcga cggtaagtga gcgcgtaacg tcgcgcttca cccgttctca ttatcgccag
     7261 ttcgacctcg atcaggcatt ttcggccaaa atctttgacc gctacctgaa tctgctcgat
     7321 tacagccaca acgtgctgct ggcaagcgat gttgaacagt tcgcgaaaaa gaaaaccgag
     7381 ttaggcgatg aactgcgttc aggcaaactc gacgttttct acgatctcta caatctggcg
     7441 caaaagcgcc gttttgagcg ttaccagtac gctttgtcgg tactggaaaa gccgatggat
     7501 ttcaccggca acgacactta taaccttgac cgcagcaaag cgccctggcc gaaaaacgag
     7561 gctgagttga acgcgctgtg ggacagtaaa gtcaaattcg acgagttaag cctgaagctg
     7621 gcaggaaaaa cggataaaga aattcgtgaa accctgacac gccgctacaa atttgccatt
     7681 cgtcgtctgg cgcaaaccaa cagcgaagat gttttctcgc tggcaatgac ggcgtttgcg
     7741 cgtgaaatcg acccgcatac caactatctt tccccgcgta ataccgaaca gttcaacact
     7801 gaaatgagtt tgtcgctgga aggtattggc gcagtgctgc aaatggatga tgactacacc
     7861 gttatcaatt cgatggtggc aggtggtccg gcagcgaaga gtaaagctat cagcgttggt
     7921 gacaaaattg tcggtgttgg tcaaacaggc aagccgatgg ttgacgtgat tggctggcgt
     7981 cttgatgatg tggttgcctt aattaaaggg ccgaagggca gtaaagttcg tctggaaatt
     8041 ttacctgctg gtaaagggac caagacccgc actgtaacat tgacccgtga acgtattcgt
     8101 ctcgaagacc gcgcggttaa aatgtcggtg aagaccgtcg gtaaagagaa agtcggtgtg
     8161 ctggatattc cgggcttcta tgtgggtttg acagacgatg tcaaagttca actgcagaaa
     8221 ctggaaaaac agaatgtcag cagtgtgatc atcgacctgc gtagcaatgg cggtggggcg
     8281 ttgaccgaag cggtatcgct ctcaggtctg tttattcctg cgggtcccat tgttcaggtc
     8341 cgcgataaca acggcaaggt tcgtgaagac agcgataccg acgggcaggt gttctataaa
     8401 ggcccgctgg tggtactggt tgaccgtttc agtgcttcgg cttcagaaat ctttgccgcg
     8461 gcaatgcagg attacggtcg tgcgctggtt gtgggtgaac cgacgtttgg taaaggcacc
     8521 gttcagcaat accgttcatt gaaccgtatt tacgatcaga tgttacgtcc tgaatggcca
     8581 gcgctgggtt ctgtgcagta cacgatccag aaattctatc gcgttaacgg cggcagtacg
     8641 caacgtaaag gcgtaacgcc agacatcatc atgccgacgg gtaatgaaga aacggaaacg
     8701 ggtgagaaat tcgaagataa cgcgctgccg tgggatagca ttgatgccgc gacttatgtg
     8761 aaatcaggcg atttaacggc ctttgggccg gagctgctga aggaacataa tgcgcgtatc
     8821 gcgaaagatc ctgagttcca gaacatcatg aaggatatcg cgcgcttcaa cgctatgagg
     8881 gacaagcgca atatcgtttc tctgaactac gctgtgcgtg agaaagagaa taatgaagat
     8941 gatgcgacgc gtctggcgcg tttgaacgaa cgctttaaac gcgaaggtaa accggagttg
     9001 aagaagctgg atgatctacc gaaagattac caggagccgg atccttatct ggatgagacg
     9061 gtgaatatcg cactcgatct ggcgaagctt gaaaaagcca gacccgcgga acaacccgct
     9121 cccgtcaagt aatatcaatc aggcacaaga aattgtgcct gattttttaa cagcggcaag
     9181 atgccgtaaa tcagatgcta caaaatgtaa agttgtgtct ttctggtgac ttacgcacta
     9241 tccagacttg aaaatagtcg cgtaacccat acgatgtggg tatcgcatat tgcgttttgt
     9301 taaactgagg taaaaagaaa attatgatgc gaatcgcgct cttcctgcta acgaacctgg
     9361 ccgtaatggt cgttttcggg ctggtactga gcctgacagg gatacagtcg agcagcgttc
     9421 aggggctgat gatcatggcc ttgctgttcg gttttggtgg ttccttcgtt tcgcttctga
     9481 tgtccaaatg gatggcatta cgatctgttg gcggggaagt gatcgagcaa ccgcgtaacg
     9541 aaagggaacg ttggctggtc aatactgtag caacccaggc tcgtcaggca gggatcgcta
     9601 tgccgcaagt ggctatctac catgcgccgg acatcaacgc ttttgcaacc ggtgcgcgcc
     9661 gtgatgcctc tctggttgct gtcagcaccg gtttgctgca gaacatgagc ccggatgaag
     9721 ccgaggcggt aattgctcac gaaatcagcc acatcgccaa tggtgatatg gtcaccatga
     9781 cgctgattca gggcgtggtg aacaccttcg ttatctttat ttcccgtatt ctggcgcagc
     9841 ttgccgcggg ttttatgggc ggaaatcgtg atgaaggtga agagagcaac ggcaacccgc
     9901 tgatctactt tgcggttgca acggttctgg aactggtgtt tggtattctg gcgagcatta
     9961 tcaccatgtg gttctcgcgt catcgtgaat tccatgctga tgccggttcg gcaaaactgg
    10021 ttggtcgcga gaaaatgatt gccgcgctgc agcgcctgaa aaccagctat gaaccgcaag
    10081 aagcaaccag catgatggct ttctgcatta acggtaagtc gaaatcgctc agtgagttgt
    10141 tcatgactca cccgccgctg gataaacgaa ttgaagctct gcgtacgggt gaatacctga
    10201 agtaatatga agtaaatact aaaaagcgcg tcctgatcga cgcgcttttt ttatgccttg
    10261 gatcgtggct gagtgatacg taaaccactg acacaggcag caatcactgc cagaatagcc
    10321 gcagccatca gcgaaacgtg cgtaccatta tcaccgaact gatttagcat cagcgccacc
    10381 agtgccgcgc cgctactctg acccagtaga cgagccgttc ctaacatgcc actggctcca
    10441 ccgctacgtt cgcgaggagc ggaggtaata atggtgtggt tattgggtga ctggaacaag
    10501 ccaaatccag cgccacataa gatcatcggc cagataatat tgatatccgc aggtgacgcg
    10561 ggcagcagaa ccaggggaaa aagccccgca gccatgatga acaaccctag agcacccagc
    10621 aatcctgcat gtacgcgttc aatcaaatag cctgccaacg gagccatcac catcgttgct
    10681 aacggccacg gtgtcagaag taaacctgtt tcgacttcac tacgcccgag cacggtttgc
    10741 aggtaaaagg gcagggaaac cattgccagc atttgtgcgc agaaagagca aacagatgtg
    10801 caaatagaaa gtgaaaacag cgggatacgc agtaaatcca ccggtagcag cggtacggga
    10861 agagaaagct ggcggcgaat aaagaaaata ccaacaacaa ccattaccac cagttccgca
    10921 gcaattaacg tcagcgattg cccctgagcg aaaccactca acgcagtgat aagcaggccg
    10981 aaggttaacg cgttcatcac ggcgctgggc aggtcgaaac ggggtttact ggcgcgagaa
    11041 ccattgggtg gcagaaaacg catcgccaga agcagggcga taatacctaa cggtacgttg
    11101 attaaaaata accatttcca ggatgcgatg gagaggattg ctgcagcaat tgtcggcccg
    11161 gcagcagaag agacggcaac aatatggcga ttgttggcag ggcgacgttg gcgattgcgc
    11221 cgtcaaggac agccatcgaa atgccaatca caatggttaa tatcgcaccg tatcgctggg
    11281 gcaatggcag gccgtcggcc tgaacttttg gcataggaat tttatatctt tggtgaataa
    11341 tcaacagggg tatgattcta gcactgatat tcacggtgga tgtcgcagaa ttgtaactaa
    11401 ccggcaaaag atggattgcg accaccctgg tgcgaaatta taataaaaaa cagttctgat
    11461 ttttataaaa cactcgcaat gaggtgataa atggctaacg cagatctgga taaacagcct
    11521 gattctgtat cttccgtgct aaaagttttt ggcattttgc aggcgctggg tgaagagcgc
    11581 gaaataggga taaccgagct gtcgcagcgc gtcatgatgt caaaaagcac cgtttatcgc
    11641 tttttacaga ccatgaaaac cttaggttat gtggcgcagg aaggggagtc ggagaaatat
    11701 tccctgaccc tgaaattgtt tgaactgggc gctcgcgcgt tacaaaacgt cgatttaatt
    11761 cgtagcgcag atatccagat gcgtgagatc tcccgcctga ccaaagaaac tatccacctc
    11821 ggcgcactgg acgaagacag tattgtttac attcacaaaa ttgactctat gtacaatttg
    11881 cgcatgtatt cacggattgg gcgtcgtaat ccgctgtaca gcaccgcgat tggtaaggta
    11941 ctgctggcat ggcgcgatct cgatgaagtg aagcaaattc ttgagggcgt ggagtataaa
    12001 cgcagtaccg agcggaccat caccagtaca gaagcgttat tacccgttct ggaccaggtg
    12061 cgcgagcagg ggtatggcga agataacgaa gagcaggaag aagggctgcg atgcattgcg
    12121 gtaccggtat ttgatcgctt tggcgtggtc attgccggtt tgagcatctc cttcccgacg
    12181 ttgcgtttct ctgaagagcg tttacaggaa tatgtcgcaa tgttgcatac cgcagcgcgc
    12241 aaaatttctg cccaaatggg ttatcacgac tatccgttct gatgagagta agaacctgtc
    12301 ggaatatcaa acagacaggt tctttattta gcatgagaaa aataaagttg aaggtgcgtt
    12361 atattaaacg cgcttgctat aagaatattt tactcatgag tgagaatctg gttgtttatt
    12421 acccttaacc attatcgacc acgatattgc ttttacgtaa cagtgggcaa tctgttatcc
    12481 ccaagaaacc acttttagtg tgcaagtatt gtaccgtgct ggtgcctctg gcagtcagat
    12541 aggtacattg caaacctaat cctgcggcat tctctttgct tccaatcaaa acgccatatc
    12601 cgctgagtaa taatcctatc cataccagtg ctatcagcat aactgtgcga atgatgaatc
    12661 gcattacaac ctcttctctt tttatgttcg cttaatcgta gcggcaatat gcgctgaagc
    12721 aagctactca ttccgaaaaa gcacgaatat cgacatagtt aggcgctgtt taactaacgc
    12781 atgctagttt aatgacataa ggtaggtgaa gcggagattg gagtgaaaaa gtttcgatgg
    12841 gtcgttctgg ttgtcgtggt gttggcttgc ttgctgcttt gggcgcaggt attcaacatg
    12901 atgtgcgatc aggatgtaca atttttcagc ggaatttgtg ccattaacca gtttatcccg
    12961 tggtgatatc attttttcag cactagggaa ggtgcgaata agcaggtcat ttcttcccaa
    13021 gctgactcgc tgattaaaat ttcgcggatc tgggccgatt tttttcccgt aaacacatcg
    13081 aatcagccta tttaggctat tttttccacc atttctggcg ttatttccgg tttttactga
    13141 gatctctccc actggcgtat catttggtcc acccgaaaca ggttggccag ggtgaataac
    13201 atcgccagtt ggttatcgtt tttcagcagc cccttgtatc tggctttcac gaagccgaac
    13261 tgccgcttga tgatgcgaaa cgggtgctcc accctggcac ggatgctggc tttcatgtat
    13321 tcgatgttga tgaccgtttt gttcttgcgc ggatgctgct tcaaggtttt taccttgccg
    13381 ggacgctcgg cgatcagcca gtccacatcc acctcggcca gctcctcgcg ctgtggcgct
    13441 ccttggtagc cggcatcggc tgagacaaat tgctcctctc catgaagcag attacccagc
    13501 tgattgaggt catgctcgtt ggccgcggtg gtgaccaggc tgtgggtcag gccactcttg
    13561 gcatcgacac caatgtgggc cttcatgcca aagtgccact gattgccttt cttggtctga
    13621 tgcatctccg gatcgcgttg ctgctctttg ttcttggtag agctgggtgc ctcaatgatg
    13681 gtggcatcca ccaaagtgcc ttgggtcatc atgacgcctg cttcggccag ccagcgattg
    13741 atggtcttga acaattgacg ggccagttga tgctgctcga gcaggtggcg gaaattcatg
    13801 atggtggtgc gatccggcag ggcgctatcc agggataatc gggcaaacag gcgcatggag
    13861 gcgatttcgt acagggcatc ttccatggca ccgtcgctca ggttgtacca atgctgcatg
    13921 cagtgaatac gcagcatggt ctccagcgga tagggccgtc ggccattgcc cgccttggga
    13981 taaaacggct cgatgacagc ggtcatattc tgccatggca gaatctgctc catgcgggag
    14041 aggaaaatct cttttcgggt ctgacggcgc ttagtgctga attcactatc ggcgaaggtg
    14101 agttgatggc tcatgatgtc cctctgggat gcgctccgga tgaatatgat gatctcatat
    14161 caggaacttg ttcgcacctt ccctagattt tcttcctctc cagcagtggt agaatagcgc
    14221 ctctactcag aggtggtgaa atgaacgaag ttgtaaattc aggcgtgatg aacattgcgt
    14281 ctttggttgt atcggtggtg gttcttctta tcgggctcat cttgtggttt tttatcaatc
    14341 gtgccagttc acggactaac gaacagattg aactgcttga ggcgttgctg gatcagcaaa
    14401 aacgtcaaaa tgcactgtta cgtcgtttgt gcgaagcaaa cgaaccagag aaagcagata
    14461 aaaagaccat tgagagtcaa aaatcggttg atgacgaaga tattattcgc ctggtcgccg
    14521 aacgataatc cttcctcaat cttgagtaac cccgtcgttg aagggttact caattcatcc
    14581 tgattatacg cactgtcatg gtgcaactgc ttcaaattaa aactctggtc tgacttcaaa
    14641 taattatcat attatttatt tgcgtaacgg gatatcagaa aaagatattt tcctcattaa
    14701 tttattagga tgtttacatc ggatttgtga ttaagcgtgg tattatttat tagcgaaacg
    14761 tttctctctt gatttttttg ctcattcatc aatttttctt attttaaatt ttcaatccct
    14821 ttggggattg acttctcttt agggtaattg atagccgtta accgactgtt ttatgagaaa
    14881 aagtgatata actttttatt cattgcatag caaaaaatgt gatattgcac gcactatgta
    14941 ataacttctc ccactggcct ggaacaactg aacttattga actatgttag aaaatacgcc
    15001 agtttaagta tctgcctgaa ctggcaaggt taagcacaat gatatatcgg cgcgtattcc
    15061 gttgcataag tgtgcaaaaa aagtggaaga cgtatcgaga tttgtgcgtc tgatcgagac
    15121 atgtttaaaa atggcttgcc ataattaacg ttgtatgtga taacagattt cgggttaaac
    15181 gaggtacagt tctgtttatg tgtggcattt tcagtaaaga agtcctgagt aaacacgttg
    15241 acgttgaata ccgcttctct gccgagcctt atattggtgc ctcatgcagt aatgtgtcag
    15301 ttttatctat gttatgcctg cgggcgaaga aaacaatcta aggaattttt caaatggcaa
    15361 agattaaagg tcaggttaag tggttcaacg agtctaaagg ttttggcttc attactccgg
    15421 ctgatggcag caaagacgtg ttcgtacact tctccgctat ccagggtaat ggcttcaaaa
    15481 ctctggctga aggtcagaac gttgagttcg aaattcagga cggccagaaa ggtccggcag
    15541 ctgttaacgt aacagctatc tgatcgaatc cactgatctg aagtgtgaat acgcttcaat
    15601 ctcgctataa agcctcgtcg aatgcgaggc tttttactat gctttatctt cgctcctggc
    15661 gttcggatat ttgcccgccg cgtgattcgc gttacacttg cggcctttag tatcctgccg
    15721 gagttgtcat gtctttttcc tgtccacttt gccatcagcc tctttcgcgt gaaaaaaaca
    15781 gctatatctg tccccagcga catcagtttg atatggcgaa agaagggtat gtcaatctgc
    15841 tgtccgttca gcataaacgg tctcgtgatc cgggcgacag cgcggaaatg atgcaagcac
    15901 gccgcgcatt cttagatgcc ggacattatc agccgctgcg tgatgcaatt gtcgcccaac
    15961 tgagggaacg gcttgatgat aaggccacgg cggtgctgga tattggctgt ggtgaagggt
    16021 attacacaca cgcatttgcc gatgcgttgc ccgaaatcac cacgtttggt ctggatgttt
    16081 cgaaggtagc gataaaagcg gcggcgaaac gctatccgca ggtcactttt tgtgtcgctt
    16141 ccagccaccg tttgccgttt tccgatacca gtatggacgc cataatacgt atttacgcgc
    16201 cgtgtaaagc agaagaatta gcacgagtag tgaagcccgg cggctgggtc attactgcca
    16261 cgccgggacc gcgacatttg atggagctga aggggctgat ttacaatgaa gtacatcttc
    16321 atgcacctca tgcagaacaa ctggaaggtt ttacattaca gcagagtgcg gagttgtgtt
    16381 atccgatgcg tcttcgcggt gatgaagccg tcgcattatt gcagatgacg ccgtttacct
    16441 ggcgtgcgaa gccagaagtc tggcaaacac tggcagcaaa agaagtgttc gactgccaga
    16501 cggactttaa tattcacctc tggcagcgtt cttattaacc gtggaagtgc gtccagagga
    16561 tctggacgcc gatgccgatc agtaccagcc cgccgagaat ttccgctttt ttcccaataa
    16621 ttgagccgat aaagcgacca accatcatcc ctaatgttga cataatcaag gttgcacaac
    16681 caatggccaa tgcggtcgcg ataatgttga cctgcaggaa agcaagacca acacccacag
    16741 ccatggcatc caggctggtg gcaatcgcgg tggttaccag tagccagaaa ccgtgtcgac
    16801 ggcgcggctc ttcatcttca tcatctgcgc cacgaaaacc ctcaataatc attcgcccgc
    16861 cgaggaatat cagcagcaca aacgcaatcc agtggttcca ttcaaggaca aaccggctgg
    16921 ctaacatgcc cattccccag ccgatcagcg gcgtcagggt ttcgacggca ccaaaaataa
    16981 ggccggttcg caatgcttca gaaaatttcg gtttatggag ggtggcacct ttaccgattg
    17041 atgcagcaaa tgcatccatc gacataccaa acgcaagaag aacagtagca gtgatattca
    17101 tgacaatgtc ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata
    17161 catgaacaac aagccgtgat ggttttcgcg ttcatgcagg catagcgatt gatgtgcata
    17221 tggtctcgcc tgactgaaag tattcagtcc gtacgtgcca cgttttgcaa cgagtatgtt
    17281 gacacgtaca tttccggaat ctggaaatcg gctactcccc aaatgagggc gcaaccttaa
    17341 catattttga gaatataaaa caactgttgg aatatattat ttcattgctt tattgataac
    17401 gattttcatt tggatttata tatcaatgaa acgttaaaat aaataacaaa taagggaaga
    17461 ggtaatatag cctaagctat gtctgattat taaaaataca ctaaacatag ctttggctaa
    17521 attcataact tattgagttg aaacgagaag cttataaatt ttttccagat cgtcgatatt
    17581 tcgaacgcgg attaacagcc gacgctgttc taattgcatc accaacacgc catcttccga
    17641 caagttcatc gctttgattc ggctatattc aatccagaca ttggcgaaga aaaaaccttt
    17701 ttgtttaaag atgatcttcg gaacgcggat ccagaatata taaaaaccca tcagagccag
    17761 tgcgcttaat aaccatgtcg ttattaacgc accatgattc gtgacgttgt tatagataag
    17821 aatgacaatc aatccgacga agataacgct atcgatgcga ccacgccgga gcaaaggaat
    17881 tgccagcagg gtggggccgt tacggcgggg catgatgaac tgatcgtaga tcgcgaaggc
    17941 caggagtgcg gcgatgaaaa gaatcagtac caggtccgtg attgtcatta atcctccaga
    18001 taaaaaaacg gggccaaaag gccccggtag tgtacaacag tcttacagtc ccagcaggcc
    18061 gcaagcgtaa ccagcgatac cgatgacgaa gaagccaacg atgatccaca gcgggttaac
    18121 ttttttgcgc agtagccaca tacaagcaaa ggtcagcagc agtggtacca ggcctggcat
    18181 taactggtcc agaatagtct ggacagtagt aacgtgttct ttgcccgtct ggtcagtaat
    18241 gcgagagaca accagcggga tgttgacatg tgtccacttg ttaaccaatg cccccatgac
    18301 aaacaggccg aggatagacg ccccttccgt cagtttttgc aggaagccac cacccatatc
    18361 tttaacgata tcgatacctt tggagtaacc atacgctacg ccgtagtaac gggttgccag
    18421 acgcaccagg ttaaacagga tgaagaacag cagcggacct aacaggctgc cgctcatcgc
    18481 gataccggca cccagtgctg caaataccgg acgtacggtt ccccagaaga tcgggtcgcc
    18541 tacaccagcc agcggcccca tcaaaccgac tttgataccg ttgatagcac cgtcgtcgat
    18601 ctctgcgcca ttagcacgct gttcttccag cgccagggtt acgccgagaa tcggcgcagc
    18661 cacgaacggc tgggtgttaa agaactccag gtgacggcga atagcttgtt tacgagcttc
    18721 gttgttctca gggtagaggc gacgaattgc cggtaccata gagaagcaga aacccagtgc
    18781 ctgcatacgt tcgaagttcc atgaaccctg gaagaggtta gaacgcagga agacgccacg
    18841 aatatcactt tgagtgagtt ttttctcggt ggtagtttga gttgtatcaa ccatttcgct
    18901 cacctgttag tccagttcgt tatcgagatc gttgttacca gctgcctgag caggcgcacc
    18961 ggctacgcgg ttgtatttcg ggctaagttg gatgtagagc actgccataa cagtaccaat
    19021 cacacccaga gcaaccaggt taaagttggt gaatgctgcg gttacgaagc cgaggtagaa
    19081 gaacggcatc aggtagccag cacgcatcat gttgataacc atcgcataac caaccaccac
    19141 gatcatgcca ccggcgatat tcagaccatt ggtcaccact tccggaatcg cattcagcat
    19201 gttctgtact tcgctggtac caacagacag cgcaacgatg acggccggaa tagccacacg
    19261 cattgcttgc aggaacagag aagaaacgtg gatccaggaa atcgctgtca ggttgccgtt
    19321 atcagcagcc ttatccgcag cgtgctggaa agcaacggta atagtacgaa cgatgatggt
    19381 cagtacctgg cccgcagcgg ccagagggat tgccagtgcg atacctgcac caatgctctg
    19441 atgacctgcg ataaccagaa tggtagaaat gatagaagcc agagcggcgt caggcgcaac
    19501 tgcagcaccg atgttcatcc agcccagcgc gatcatttcc agcgtaccac cgataataat
    19561 accggttttc atatccccaa gaacgatacc caccagggta cacgcgatta gcggacggtg
    19621 aaactgaaat tcatcgagga ttgatcccat acctgcgata caggctacga taaataccag
    19681 cacaatttga agagtggtaa tctccattgt acttctcctg tttacgtaag acttaagtgt
    19741 gaaaactgag tgataatcaa cacaatacgt tacttatcga ttttgctgat cagatccatc
    19801 attttcagtt tcggatcggt ggaaacctta cggacttcca gctcaatacc gcgcgcattc
    19861 agtttcttga acgcctcgat atctttttca tcaaccgaaa ccgcgttatt cacctgggtt
    19921 ttaccctgac ggaatgccat accaccgacg ttaacagagg tgattttcac gccgccttca
    19981 acgagacgct ctacatctgt tgggttggta aataacagca ttacgcgttc gccagcatat
    20041 ttcgggttgt tgtagacgcg aatcattttg gcaacatcaa ctacgtgtgc tgttacgccc
    20101 ggaggtgcaa cctgggtgag cagtgtctta cgaacggtat ccgcagccac ttcatcacta
    20161 acaacaataa tacgggagac attggtttct ttggtccagc gggtggcgac ctgaccgtga
    20221 atcagacggt cgtcgatacg cgcaaggcca ataaccatgt agtcgtttgg ccccattggt
    20281 tttgccggag ttggagccgc ttttggtgct gcggcagcgg gtgctggcgc ggctttttca
    20341 accggtttgg ctttcagtgc tttcacgcct tcacggcctg tttctactgc cagcgcaacc
    20401 agttcatcaa agcttgggtc atcatcacga gccattaacg tttccacgag cattggaatg
    20461 ttaacgcctg caatgacttc ataatgctct ttgtcgacga caatgcggct ggcagcattg
    20521 aacgggctgc ctccccatgt atcaacgaga aacagcacgc ctttagtggt gtcgagtttt
    20581 gccaactgag cgttgtactt ttcaatcagc gtttcggcat tttcacctgg aacgaaatcg
    20641 atccagccga cgttttcctg ctcgcctaac agcatttctg ccgttttaag caactgctct
    20701 gcagcccaac catgtgtgcc tataacaata gcaatggtca cttgctacct cctttattat
    20761 cgttaacacc tcaacgtgcc agatgtattt ttgaatcgtc gtccacaatc gaatcgattc
    20821 agataagggc tacaaccaaa aacccctgcg tttcgcgatt tattttagat atcgaaaaaa
    20881 ttattttatg tgatgaagat ccgtaattta actttcgatt agcagaaatt tcgaaaggta
    20941 aaatatcctt gtcacattcg tttgcaaagg aaggtaaatc tttgccaaat cagaggcgtc
    21001 tctgatatgt ttaactcccg tttaacaacc atggagtata gggcagtagc ccgcagtatg
    21061 gatcgtcacc gacgtcattt cagcatcagg ccttttaacg cctgcctttc tggcactcta
    21121 tgccgcacct ttcgtttgca ttttgtcgtt acgcctgcat tatttctggc gtcgaatagc
    21181 tattccttaa gcaggagctt gtcatggaat tcttaatgga cccctcaatt tgggcggggc
    21241 tactcacgct tgttgttctc gaaattgtgc tgggtatcga taacctggtc ttcatcgcca
    21301 ttcttgctga caaactgccg ccaaaacaac gcgataaagc gcgtttgctg gggttatcac
    21361 tggcgctgat tatgcgtctg gggctgctgt cgctgatttc atggatggtc acgctgacca
    21421 aaccgctatt taccgtcatg gatttctcct tctccggacg cgacctgatt atgttgttcg
    21481 gggggatatt cttgctgttc aaagcaacaa ccgaactgca tgaacggctg gaaaaccgcg
    21541 atcatgattc cggccacggt aaaggctacg ccagtttctg ggtggtcgtc acacagattg
    21601 tcatccttga cgccgtcttc tcgttggatg cggtaattac tgcagtaggg atggttaacc
    21661 atctgccggt gatgatggcg gcggtagtga ttgcgatggc ggttatgttg ctggcatcca
    21721 aaccgctgac gcgattcgtt aaccagcacc ccacggtggt ggtgctctgt ctgagcttcc
    21781 tgttaatgat tggtctgagt ctggtggcag aaggtttcgg tttccacatt ccgaaaggtt
    21841 acctgtatgc cgcgattggc ttctcgatca tcatcgaagt gtttaaccag attgcgcgtc
    21901 gcaactttat tcgccaccag tcgactttgc cgctgcgagc gcgtactgcc gatgccatcc
    21961 tgcgtttgat gggcgggaaa cgtcaggcca atgttcagca cgatgccgat aacccgatgc
    22021 cgatcccgga aggtgcattt gccgaagaag aacgttacat gattaacggc gtactgacgc
    22081 ttgcgtcgcg ttctctgcgc gggatcatga cgccgcgcgg tgaaataagc tgggttgacg
    22141 ctaatctcgg ggtcgatgaa atccgcgagc aactgctctc ttcaccgcac agtctgttcc
    22201 cggtatgtcg cggtgaactg gatgaaatca tcggtattgt acgtgctaaa gaactgctgg
    22261 tggcgctgga agagggcgtt gatgtggcgg cgattgcttc ggcgtctccg gcgattatcg
    22321 tcccggaaac cctcgatccg atcaacctgt tgggcgtgct gcgtcgtgct cgcgggagct
    22381 ttgttatcgt gaccaacgag tttggtgtgg tacaaggtct ggtcacgccg ctggatgtgc
    22441 tggaagccat tgcgggtgaa ttcccggacg ctgacgaaac gccggaaatc attactgatg
    22501 gtgacggctg gctggtaaaa ggcggtacag atttgcatgc cttgcagcag gcgcttgatg
    22561 ttgagcacct tgccgatgac gatgatatcg cgacggtcgc gggcctcgtg atctcggcaa
    22621 atggtcacat tccccgtgtg ggcgatgtga ttgatgtagg gccactgcat atcaccatca
    22681 ttgaagccaa tgattatcgt gttgatctgg ttcgcattgt taaagagcaa ccggcgcacg
    22741 atgaagatga gtaagcatta acgtaacggc ataatgggcg tgatatgtcc attatgccgg
    22801 gcgggcggcg gttggctgcc cgccagccat ttgggaaaat cacgtagcgg catcggctgt
    22861 gcgtacaaat acccttgcaa aatatgcacg ccatgacggc gcaaatactt cgcctgttct
    22921 tgcgtttcca cgccctccgc caccagttca atattcagtc tttgccccag cgcgatgatg
    22981 atatcggtca ccgtcgagtt aaccgcgtca gaacctatag ctgcggtaaa tgacttatca
    23041 attttcagca tgtcaggacg taatgtttca agccaggaaa atgaactgtt gccggtgccg
    23101 aagtcatcaa tcgccagttt gacgttttta cgatgtagct cgcgggcaat ccgataatca
    23161 acatccagta aggcatcgcg ttcggtgatt tcgaggatca gttgctgaat cgggtgagcg
    23221 ctaaaccagt actgattgag atcttttatc agcacaccac ggcgaaaatg gctgggtgcg
    23281 acgttaatgc caacatgaaa ctgactactc atcgggaaaa catggcgctg acgaatggtt
    23341 tctgccatca catagcgggt cagtggcaca attaaatgat gttcttccgc gataggaata
    23401 aacacatccg gtgaaatcca gccctgacgc ggattgttcc agcgcagcaa aatctctaca
    23461 ccaatacatt gctggctgcg cgcattaagc aaaggctgac agaacaattc gaactcatgt
    23521 tgcgccaggc ccagattgat ttcgcgggaa aagctcatcc ggtaagcggt cgccagccag
    23581 gcgatgtagc ccaccagtaa acttagcagc accgccagcg gtaattgtgt tggaagatag
    23641 tgccatgcca gcgccgtagc ccccggacca ttaacgttaa tggtaaaagg aaagcgttgc
    23701 gaagaaacct gatagttttc attgtcttca ggttgcggaa gggaatctac cagcccatta
    23761 ccatagagca aatgccgttt gtccaccgtc aggctggcgc tactgatttg cggcagttgt
    23821 ggctcaagga gcattgccgt cagtaagtcg atgttaatca tctccatgac cccagcattg
    23881 ctactgcccg ctgcaggcgt ccattgaatc aaaaccggac tgcctttaat cagggcacgg
    23941 tcgatcgtca ggcgtaaaag tggttgcggt gcaggaagtt cagccagaat gtccacgacg
    24001 ggcacattgc gataaccaaa aatgctggag caatagagtg ttccgtcttg caccaggcca
    24061 atggatcgca cagtttggag ttttgccgcc tgtttacgta atggcaaatg ggcgacagag
    24121 cagggcagac caatcagcgg aagcaagact tcgctacctg cctgtagggg aagcagtact
    24181 ttatctaatt cctctacagc gtgattggcg aattgtacta cccgttgttg atttaagtta
    24241 cgctgtgaaa taaatcgcac gctcagggtc gaagcgagcg taacgagtgc gcaaatcgta
    24301 caaacaatca ttcgattacg gcgataggtt ttaatgatcc gttgtgcttt ttgcatgcgg
    24361 gccaccctgt aagcgtatgg cgacaaacgc ctgacgcaac agtggaagtg tagtggggaa
    24421 tattacagca gacgagaaag cgggtataaa ttcgcccatc cgttgcagat gggcgagtaa
    24481 gaagtattag tcacactgga ctttgattgc cagaccaccg cgtgaggttt cgcggtattt
    24541 ggcgttcatg tccttaccag tttcgtacat cgtttcgatg accttatcca gcgagacgcg
    24601 cggtgcactg gtgcggcgca gagccatccg tgcggcgtta atcgccttca cagaggcaat
    24661 ggcattacgc tcaatgcacg gcacctgaac ctgccctgca accgggtcgc aggttaaacc
    24721 aaggttgtgt tccatgccaa tttccgccgc cacgcaaacc tgttccgggc taccgcccag
    24781 cagttctgca agacccgcag cagccattga acaggcaaca cccacttcgc cctggcaacc
    24841 aacttccgca ccggaaatag aggcgttcat tttatacaat gcaccaatcg cgcccgctgc
    24901 cataaagtaa cgggtataga tgtccgggct gaccgattca ataaagtggt catagtaagc
    24961 cagcactgcc ggaacgatac cgcaggcacc gttggttggc gcagttacca cacgaccacc
    25021 ggcggcgttt tcttcgttaa ctgccagcgc aaacatgttt acccagtcaa tgacattcat
    25081 cggatcgtta gacagtttat cgctggaaac taacatccgg cgcagggcag acgcacgacg
    25141 tggcacgcgc agcgggcctg gcagtacacc ttcggtgttc atcccgcgat cgatacatgc
    25201 ctgcatggtt tgccagacat gcgcgaaata ctcgtcgatc tctttcttgc tgtgcagcgc
    25261 cagttcgttc tgcatagcga gaccagacag cgaatagccg gtttcattac agtacgcgag
    25321 cagttcggtg gcagatttga acggatacgg cacgctcact tcgttggcag catcctgacc
    25381 aaagtgttct tcatcgacga taaaaccgcc gccgatggaa taataagttt tgctgtagac
    25441 gacttcatcg ccgttatagg cgtggatttg cataccgttt tcatgcagcg gcaggttgcc
    25501 gttatgaaaa cgcatcccgt tgtcgcgcgg gaaatccact tcatgccgtc cctgtgccag
    25561 cagcagacgt tcgcgctctt ctacgtcgcg aataaaaccg ggaatactgt cgatatccac
    25621 ggtggcaggt tcgttacctg caagacccat aataatggcg atatcggtgt ggtggccttt
    25681 acccgtcagc gacagtgaac cataaacgtc cacggcaacg cgagtaacgc tatccagtaa
    25741 gcctttttcg accagatcat cgacgaactg tttacctgcc ttcataggcc ctacggtatg
    25801 ggaagatgag ggaccaatcc ccaccttaaa catgtcgaat agactaatca cgataatact
    25861 cctgacaagg tgactggact tccagtaacg aactaataac agcgcatagt gtaagaggga
    25921 acgcggcgac tggcttaact attcacatga attaaactaa tcaaaaaccg cgatttaact
    25981 aatattgcga tgattgtctc aagtagtgtt ttgtagatgt aaagataagt atagtcaggg
    26041 tttcacacca atttgcagcg ccagctcacg aattatgcct gcggtcattc cccatacaaa
    26101 atactgttcg taccaggaca gccatacccg atgtgaatcg ccacggcggt agatatctaa
    26161 agggtgataa cgacccagat gtaatgcctg ggcgagcggc atttcaaaca ccgccgagac
    26221 ttcatcttca ctggcgcgat acggcagatc gggcgggata atgccgacca ctggggttac
    26281 ctgatagcca gttacgctat cgacgggcgg cagcacgccg ataacttcaa cggcggaagg
    26341 cggtatagcg acctcttctt cagcttcgcg cagcgcggcg gcgataactg atgcgtccgt
    26401 gtcatcgact gcacctccag ggaatgccac ttgtccagcg tgtttacgca gatgaattga
    26461 acgctgagtc agcaacaacc ccggttgcgg tcgacggacg atggggatta acacggcagc
    26521 ctgacgatga tttagggttt cccggttaat ttgcgggcgc aaaagttgaa agcgcgataa
    26581 aaaatcatca agcatcaggc tacggtattc cacgtcttac ttctccagtt ggcgtaatat
    26641 cttattaact ttatcaaaag tttcctgata ttccgcttct tcctggctat cggcgacaat
    26701 tccaccgccc gcagagcagt atatttgtcc gttaatggca gtcagagtgc ggatagtaat
    26761 gctggtatcc atgttgccgc aaaagctcaa atagccaatg ctgccgcacc aggcattacg
    26821 tcgctgcggt tccagttcgt cgataatttc catagcccgt actttcggag ccccggttat
    26881 tgagccacca ggaaaagctg cgcgcagcag atcgctggcg tgtaactgtt ctggtagttg
    26941 tgccgttatg gtgctgacca gatgatgcac ggcagggaag ggttccacca cgaacagctc
    27001 tggtactttt accgaacctg ctacggcaac acgaccgata tcattacgca ttaaatcgac
    27061 aatcatcaga ttttcggcac gatctttcgc tgagttcgcc agtttttctg cttgtttgct
    27121 atcttcctga ggatcgggca ggcgtggtag cgtgccttta atagggcggg tctggatttc
    27181 actattatca caaagaataa accgctctgg cgaaaggctt aaaattgcac cctgttcaag
    27241 acgtaaaaaa gcgctaaatg gcgcgcggtt ggcctgatta agctgaagga atgcctgcca
    27301 ttcatcgcca gaataggtcg catgaaaacg ctgggcgaga tttacctgat agcaatcacc
    27361 gctgtgcaga tattcctgta cctggcgaaa tttttcgccg tactgctcgc gggtcatatt
    27421 ggattgccag tcggaagtaa gcgtgaaatc ttcctgcggc gagaattgct ggctttccag
    27481 ccaggcccga cgggcattga catcattatg actcagcaaa gaaactgtat gacgctggtg
    27541 gtcgacaatg agcgcccaat cgtagatacc cactgccata tccggcagaa cgatatcttg
    27601 ttccgcaatt tctggcagtg actcaaaacg gcggcccaga tcgtagccaa acaaccccag
    27661 tgcgccgccc tgaaatggca aatcttcgtt atgcgttggg cgaatgtctg cgcgatccag
    27721 cacctgctgg agcacctgta gcgggtcatc agtggtcgtt gtgcgttttt cgctttcact
    27781 aacaacggtt tctttaccta aagtggttaa agtgcaaatc ggatcggcga ccacaatatc
    27841 aaagcggcta tacggatgat cggcatagcc ggagtgtaaa agcatcgccc acggcaggtg
    27901 gcttaagcgg gagaaataaa attcagcggc gtcctgacgc cagggtaaag taatcacagc
    27961 gggagataac gtcttcatca gtcctgactc tactggctat gtgccgggta gcatgaaata
    28021 attagcgcca acaatttagc aggagttaac aatgtttgca ggtttacctt cactcaccca
    28081 tgaacagcag caaaaagctg tcgagcggat ccaggaactg atggcacaag ggatgagcag
    28141 cggccaggca atcgcgttgg tggcagaaga gctgcgtgcc aaccattccg gcgagcgaat
    28201 cgtggcgcgt tttgaggatg aagacgagta acacactaat ccgtattgat ttcgttatac
    28261 aagccgatga ttatttgatt gtgagaaaaa ggacgctttt tatatgtgat taaaagcgtc
    28321 ctgatccatt tatgctgatg taaattttac tcagaccagt gcttttagtt cttcctgcat
    28381 atatgttgaa aagtattcag gatttttaat cacagagcgt tcacctgact ccattttttt
    28441 tgcccatcca accatttttt ccgtaaaccc agggttccag ccttcctggt cagctcgcgc
    28501 cgtaatatca gcggcactcg caggtactcc cagttctttt aaatatttaa agattccctt
    28561 cgccgtgctt tcattcatcg aacttggtgt tggcgctgat acatccatgg caccaatgaa
    28621 atccagggct ttatctatta cagcaggcat attcttatcc ttaaaattaa ccatgttatc
    28681 gataaggcta tatatgacct ta
//
LOCUS       DAEEKQ010000035        28678 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.035, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000035 DAEEKQ010000000
VERSION     DAEEKQ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28678
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.035"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1046)
                     /locus_tag="KLK57_001517"
     CDS             complement(<1..1046)
                     /locus_tag="KLK57_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HBE0891917.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
     gene            1537..2094
                     /gene="pinE"
                     /locus_tag="KLK57_001518"
                     /pseudo
     CDS             1537..2094
                     /gene="pinE"
                     /locus_tag="KLK57_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA recombinase"
     gene            complement(2433..3281)
                     /locus_tag="KLK57_001519"
     CDS             complement(2433..3281)
                     /locus_tag="KLK57_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HBE0891918.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            3490..4125
                     /gene="yfhb"
                     /locus_tag="KLK57_001520"
     CDS             3490..4125
                     /gene="yfhb"
                     /locus_tag="KLK57_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HBE0891919.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            4150..4686
                     /gene="tadA"
                     /locus_tag="KLK57_001521"
     CDS             4150..4686
                     /gene="tadA"
                     /locus_tag="KLK57_001521"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HBE0891920.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQPSTD"
     gene            complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="KLK57_001522"
     CDS             complement(4683..6239)
                     /gene="mltF"
                     /locus_tag="KLK57_001522"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HBE0891921.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVSQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQCLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            6497..10384
                     /gene="purL"
                     /locus_tag="KLK57_001523"
                     /gene_synonym="purI"
     CDS             6497..10384
                     /gene="purL"
                     /locus_tag="KLK57_001523"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HBE0891922.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFKMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNTLLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGINADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            10942..12369
                     /gene="qseE"
                     /locus_tag="KLK57_001524"
     CDS             10942..12369
                     /gene="qseE"
                     /locus_tag="KLK57_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HBE0891923.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSS
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            12534..13247
                     /gene="qseG"
                     /locus_tag="KLK57_001525"
     CDS             12534..13247
                     /gene="qseG"
                     /locus_tag="KLK57_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HBE0891924.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPTGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            13237..14571
                     /gene="glrR"
                     /locus_tag="KLK57_001526"
     CDS             13237..14571
                     /gene="glrR"
                     /locus_tag="KLK57_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HBE0891925.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            14632..14970
                     /gene="glnB"
                     /locus_tag="KLK57_001527"
     CDS             14632..14970
                     /gene="glnB"
                     /locus_tag="KLK57_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HBE0891926.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="KLK57_001528"
     CDS             complement(15015..16205)
                     /gene="hmpA"
                     /locus_tag="KLK57_001528"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HBE0891927.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWHRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            16533..17786
                     /gene="glyA"
                     /locus_tag="KLK57_001529"
     CDS             16533..17786
                     /gene="glyA"
                     /locus_tag="KLK57_001529"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HBE0891928.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARFPVYA"
     gene            complement(17984..19177)
                     /locus_tag="KLK57_001530"
                     /pseudo
     CDS             complement(17984..19177)
                     /locus_tag="KLK57_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
     gene            19295..22572
                     /locus_tag="KLK57_001531"
                     /pseudo
     CDS             19295..22572
                     /locus_tag="KLK57_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
     gene            22669..23652
                     /locus_tag="KLK57_001532"
     CDS             22669..23652
                     /locus_tag="KLK57_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HBE0891929.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTLGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYISAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLNKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            23675..25186
                     /locus_tag="KLK57_001533"
     CDS             23675..25186
                     /locus_tag="KLK57_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HBE0891930.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            25211..26209
                     /locus_tag="KLK57_001534"
     CDS             25211..26209
                     /locus_tag="KLK57_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0891931.1"
                     /translation="MSASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            26242..27336
                     /locus_tag="KLK57_001535"
     CDS             26242..27336
                     /locus_tag="KLK57_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HBE0891932.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            27348..28220
                     /locus_tag="KLK57_001536"
     CDS             27348..28220
                     /locus_tag="KLK57_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBE0891933.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(28268..>28678)
                     /locus_tag="KLK57_001537"
     CDS             complement(28268..>28678)
                     /locus_tag="KLK57_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBE0891934.1"
                     /translation="RYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQYMAS
                     LGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDMTGDA
                     VGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
BASE COUNT         6818 a         7092 c         7790 g         6978 t
ORIGIN      
        1 aatgcccggg aattctcctc ggtggaaaaa gaagtccatt gtgtagaaac ctcccgacga
       61 tctgcttcag gaaaccaggc tgccacagtt tcgggaagag gggggatctg aaagttctgc
      121 cccccttcag tgatgactct gatatggcgc gaatgtaaat tttgcggcaa catctggcgt
      181 aaaaacagtc tgttttggtc tgacagggaa atacgaaact gaggcccgtt aaaatttgga
      241 accgaagttt gctgtattaa gaatagcaga gtttgatctg ataaggggtt atttgcaatc
      301 tcaatagtta tatgaggatc taaacgaaaa agatttgtcg ggattgtagt tatttggttg
      361 ttttcaaaat aaaaaaatct tctcatgata atattttctt ggatgtttgg taagttgctt
      421 aatctattgc tatatgcaaa aagaagttgc agtctcctag gtaaaggggg cagttcagta
      481 agatggttgt gcgcaacctt aagtatctta agattagaag gcagagatgg aagagtttgc
      541 agttcgttgt tcctaacctc aagttttttt aaattcaagg gtaatgaagg cagtgtggat
      601 aacctattat actctgcgct tagtgatttg atggtgtctg gtaataacgg cagattctct
      661 aattgatttt cggacacatc caaagttatt agataatgtg gtaatgcaga tatatgtgtt
      721 aagaaattat ctcttgcaat aagttcttta atattttcag gtaaatcagt aaggatggtt
      781 aatttattac agctcacatc aagcttttta atgcaagcag ggagtacagg taacgaggaa
      841 agcgccaagg ctcgcaggtc cagtttatgc cccttagagg taagacatgc acgaagtctt
      901 cttaccgcct cagtacgctg ttcttccggt aacccctgtt tctcccaatc atcccattcg
      961 gacaaataat ggttggggga tgtaattgtg ctgatatgtg gaaataaaca ggatgaaata
     1021 tttctgagca tattaatttc tcgcatatgg atatggaatt agattgtgaa taaattgtat
     1081 ttaatgtatc tctcctaatg aggagggtga ttcagaatat tcgccataag ggaatgagtg
     1141 ttatatcatg ctttctaccc tggtaagtat atttgctttt ttcgtatatt tggttcatct
     1201 ttttttgatg ttttacatat tcacaatgca gaggatttat accagaagac attagtcttg
     1261 agtaagatac tacaacatct ccaactattt tagccagcct gactacttga cttggtaata
     1321 tttgttgcta ataatcatgt ataaaataag cttatataca tagtaacttc agcaaaggat
     1381 ttagtaatga tccaaatcat atctcaatgt actgtttttt tctttaaaaa aacagtacat
     1441 tctactttat aatgactgtg tattcccatg gaaggagatc acccaattct cccccccgac
     1501 ttttgacgca gtgtcttgtt aacaggggga tttaccatgc ttattggcta tgtacgcgta
     1561 tcaacaaatg accagaatac cgatctacaa cgtaatgcgt tgaactgtgc aggatgcgag
     1621 ctgatttttg aagacaagat aagtggcaca aagtccgaaa ggccggggct gaaaaaactg
     1681 ctcaggacat tatcggcagg cgacacactg gttgtctgga agctggatcg gctggggcgc
     1741 agtatgcggc atcttgtcgt gctggtggag gagttgcgcg aacgaggcat caactttcgt
     1801 agtctgactg attcaattga taccagcaca ccaatgggac gctttttctt ccatgtgatg
     1861 ggtgccctgg ctgaaatgga acgtgaactg attgttgaac gaacaaaagc aggactggaa
     1921 gctgctcgcg catagggacg aattggcggt cgccgtccca aactttcacc agaataatgg
     1981 gcgcaggccg ggcgactaat tgcatcagga gtttctcgcc agaaggtggc gatcatctat
     2041 gatgttggcg aatcgacttt tttgtataag aggtttccag ccagggttat ataaaaccgc
     2101 agacacggcg tatacaagaa cgtgctacgg ttgactgaaa agttaatgtg gtatgtcaca
     2161 ggccttttgc gccgtaaagc cccgtccttt agggcgcgga ggacgttaaa gaaagtggtg
     2221 ttctttgtgt tgctgaactt atgcaaaatt tttttgcacc ataaatccaa caacacattc
     2281 aataacacat tgctgtatca ctatgaaaat aaatgtgttt ttatttgatt tcacacatat
     2341 cacaattgat gcatttttta gtgattaaca acgccataag gaaactctca aaacaacaca
     2401 aaccgggcgg gcattgtacg cgcattcttt actcagacca gttttttcac cagcgcttca
     2461 ctatgacgaa tacgttctgg tgccagttcc agatcctgct gaatcagcgc aatgaacagc
     2521 aaatccgtta acattccctg agcgtgacaa gcagagattg aagcactgtt tgtcgcttgt
     2581 tcttcggcaa tggtatacag gcaatgagaa gcacgctgtt gcagggcatt cggagtaaag
     2641 ccggtaatcg ccagcacttt tccgcccact cgcagcatct catctgccgc caggtttaac
     2701 tcgcgtcgta caccggtgta ggaaatggct aataacagat cgtcagggga cgacgcctgt
     2761 actgttgcga gtagcgcatg catatcgcgc actgcggcag cattgaagcc aatcttcatc
     2821 agcttccagg caaagttttg cgccaccaga cccgaagcgc caataccggt cagaattatc
     2881 cgccgcgcag agcgcaacat tgttacgcat tcatgcagtt tctcttcact attaacgttt
     2941 agcgttgcgt acatagcggc agtattttct ttaatcagtt tttcgccgac cagccgtaac
     3001 ggatcatcac cgcggatttg gttatgaatg ggcacggagg gcgattccgg ctggcttgcc
     3061 agcgcttcac tcaacgccaa cttaagcgcc ggaaaacctt tatagccgag tttttgcgcg
     3121 aacttcacga cgctggactg actgactccg gcttcgttgg ccagttgctg agagcttaaa
     3181 tggcgcgccg tatcaggttg tagcagcaga taatccgcca gttttttatc actctgggca
     3241 agcccctggt aacgctgacg gatacgaagt aagccgttca tatctttcct cgcgatgaaa
     3301 tagtggcgac aacaggcaat atgagacaca cgcgagtgaa ttttatattc cattgccgct
     3361 aattgccatg aattaaaaat tccagttagc catgatgcgg tcgattgttg ccggaaaagt
     3421 gatgatctat ctgttaggct atgtagtagc gaaaattaat caggatattt cagtccagag
     3481 gagtatggtt tggcaactca cgagcgtcgt gtggtgtttt ttgacttaga tggaacattg
     3541 catcagcagg atatgttcgg cagttttctg cgctatttac tgcgtcgcca accgctgaat
     3601 gcgttacttg tcctgccgtt gttaccgatt atagccattg cgttattgat aaaaggtcgt
     3661 gcggcacgct ggccgatgag tctgcttctg tgggggtgca cttttggtca cagcgaagca
     3721 cgtttacaga cgttgcaggc cgatttcgtg cgctggtttc gcgacaatgt taccgccttt
     3781 ccgctggttc aggagcgatt aaccacctac ctgttaagtt ccgatgctga tatctggttg
     3841 attaccggct ctccgcagcc gctggttgaa gcggtttatt tcgatacgcc ctggctgccg
     3901 cgggttaatc ttatcgccag ccaaattcag cgtggctatg gtggttgggt attgacgatg
     3961 cgttgtctgg gacatgaaaa ggtcgcacaa ctggagcgca aaatcggcac tccgctgcgg
     4021 ctgtacagtg gctatagcga cagtaatcag gacaatccgc tgctttattt ctgtcagcat
     4081 cgttggcgag taaccccgcg cggtgaactc cagcaactgg aatagagtaa agcatagcgt
     4141 ccgtgtataa tgcgccgcgc ttttataacc ggagttttct ttttgtctga agtcgaattt
     4201 agccacgaat actggatgcg tcacgcgctg acgctggcga aacgtgcctg ggatgagcgg
     4261 gaagtgccgg tcggcgcggt attagtgcat aacaatcggg taatcggcga aggctggaac
     4321 cgcccgattg gtcgccatga tcccaccgca catgcagaaa tcatggccct gcggcagggt
     4381 ggtctggtga tgcaaaatta tcgtctgatc gacgccacgt tgtatgtcac gcttgaacca
     4441 tgtgtaatgt gtgccggagc gatgatccac agtcgcattg gtcgcgtggt ctttggtgcg
     4501 cgtgacgcga aaactggcgc tgcgggatct ttaatggatg tgctgcatca tccgggtatg
     4561 aatcaccgag tggaaattac ggaaggaata ctggcggatg agtgcgcggc gttgctcagt
     4621 gacttctttc gcatgcgccg ccaggaaatt aaagcgcaga aaaaagcgca accctcgacg
     4681 gattaatttt gtttctcttc actccctttc ctggaaaaca gcagagaggg agaatgggtc
     4741 aaatagtttg atgacgactg ggaaagaaac gagagaaaag gaaatttctc tttgcccaac
     4801 tccgtaggcg ataccgccgg ataatcctgc gccagttgca tcgccgcttc tgtagcctgc
     4861 ttctctttct cttgcagata acccaccagg ctaatctgat acttacgaat attttcgaca
     4921 taagcgtagg cttcatgtcc acgagcgtag ccgtaagtca gcttgctgta atagggtttc
     4981 tggctaagta aaggcagaca ctgttttacg tcagcccaac tgtcaggatt ccctttggtt
     5041 tttgccgtca gggcgcgggc atccagcata tgcgcatagc ccatattgta cgcagcgagg
     5101 gcaaaccaga tccgctcgtt ctccggcaca ctttccggca ctttactcat catatcctgc
     5161 aaataacgca cgccaccgct gatgctctgt tcggcatcgg tacgatccgt aatgccgagg
     5221 ctttgcgcgg tatttttggt taacatcatc atgccgcgca cacccgtcgg tgaagtggcc
     5281 tgtgcatccc agtgcgattc ctgataagca atagcggcca gcaaacgcca gtcaatttct
     5341 tcggcgtatt tctcaaacag gggctttaac tgcggcagta ccgcatcgac ggcgcgtaaa
     5401 aatgtgcgcg tatcgacgta atcaaaatca tcgccatgcc ccaggtattt ctcttcaatg
     5461 cgtgccagcg taccgtcttc attcatttcg ttgaagaagt cgagcagggc ggcggaaagg
     5521 gtattatcgc catctaacgg gctaaaccaa gtcaccggtt gttcatcggt gatatcgagc
     5581 gctacggcga gctccgggtg aacgcgctga aacaggctga tggcgacaga atcagcaatg
     5641 gtgtaatcga gttttccttc gatgacatct tccattaatt ccgcagagcc ttttttgtcg
     5701 tctaccttcc agcttaattc cgggaatttt gtgtctttca gggtttggag atcgttaacc
     5761 accacgtgac ccggtgcaac ggtgagttgc tccgccgtca ggttgcccag tgtgcgcggg
     5821 cgatactgac tcactttata aaccagttgt tgtgacacgg aataataggt agggccaggc
     5881 tgataatttt ttacccgctc actgttatag accagtcctg ccgccagcag gtcggcgtta
     5941 ccattatcga ggtcgtcaaa cagctggctg atattctgcc gcacggtcac tttcagtttt
     6001 acaccgaggt aatcggcaaa ctgtttcgcc agttcgtaat ccaggccaaa aggtttcccg
     6061 ttgatttcgt tataagtcag gggagtatga atggtgctca cacgcaactc tccccgcgct
     6121 tgaatggcgg cgatacggtt gtcggcttta ccaaaccagg gaatggatgg ccagagagcg
     6181 accgcgagca gcagtgccag aatgccgatg aacagataat taatctttaa ttttttcaat
     6241 tagttaattc tctgtgtcgt gcgcgtccca gcttgaaaaa acgtaataat agtgaaaggt
     6301 ttactcataa atgagcggca ttttgcgtaa acctgcgcca gatggcaact tattacagcc
     6361 attggcggca cgcgttgcta attcacgatg gtgattttat ttccacgcaa acggtttcgt
     6421 cagcgcatca gattctttat aatgacgccc gtttcccccc cttgggtaca ccgaaagctt
     6481 agaagacgag agacttatga tggaaattct gcgtggttcg cctgcactgt cggcattccg
     6541 aatcaacaaa ctgctggcac gttttcaggc tgccaggctc ccggttcaca atatttacgc
     6601 cgagtatgtc cattttgctg acctcaatgc gccgttaaac gatgatgagc acgcacaact
     6661 tgaacgcctg ctgaaatatg gcccggcact cgccagccac gccccgcaag gcaaactcct
     6721 gctggtgacc ccgcgtcctg gcaccatctc tccctggtct tcgaaagcga ccgatattgc
     6781 ccataactgc gggctacaac aggtaaaccg ccttgagcgc ggcgttgctt actatataga
     6841 agccggtacg ctgaccaatg aacaatggca gcaggttacc gctgaactgc acgaccgcat
     6901 gatggaaacg gtcttttttg ctttagatga tgcagagcag ttgtttgccc accatcaacc
     6961 gactccggtt accagcgttg atttgctggg gcagggccgt caggcgctga tcgacgctaa
     7021 cctgcgtctt ggcctggctc tggcggaaga tgaaattgac tatctgcagg atgctttcac
     7081 aaagcttggt cgtaacccga acgacatcga gctgtatatg ttcgctcagg cgaactctga
     7141 gcactgccgc cacaaaattt ttaatgccga ctggattatc gacggtgaac agcagccgaa
     7201 atcgctgttc aagatgatca aaaatacttt cgaaaccacg ccagatcacg ttctctctgc
     7261 ttataaagat aacgccgccg taatggaagg ttctgaagtg ggccgctact ttgctgacca
     7321 cgaaacgggc cgctacgatt tccatcagga accggcgcat attctgatga aagtcgaaac
     7381 tcacaaccac ccgacggcga tttctccgtg gccgggcgcg gcgacgggtt ccggcggcga
     7441 aattcgtgat gaaggcgcca ccggacgcgg tgcgaagccg aaggctggtc tggttggttt
     7501 ctccgtttcc aacctgcgaa ttcctggctt cgaacagccg tgggaagaag atttcggtaa
     7561 gcctgagcgc attgtcaccg cgctggacat catgaccgaa ggcccgctgg gcggcgcggc
     7621 gtttaacaac gaatttggtc gtccggcact gaatggctac ttccgtactt atgaagaaaa
     7681 agtgaacagc cacaacggcg aagagctgcg cggttatcac aaaccgatca tgctggcggg
     7741 cgggatcggc aacattcgcg ccgatcacgt acaaaaaggc gagatcaacg tcggtgcgaa
     7801 gctggtcgtt ctcggcggcc cggcaatgaa catcggtctt ggcggcggag cggcgtcttc
     7861 catggcgtca ggtcagtctg atgccgacct cgactttgct tccgtacagc gcgacaaccc
     7921 ggagatggag cgtcgctgcc aggaagtgat cgaccgttgc tggcagcttg gtgatgccaa
     7981 cccaatcctg tttatccacg acgtcggcgc tggcggtctt tctaacgcga tgccggaact
     8041 ggtgagcgac ggcgggcgcg gcggtaaatt tgaactgcgc gatattctaa gcgacgaacc
     8101 gggcatgagc ccgctggaaa tctggtgtaa cgaatcccag gaacgctacg tgctggcggt
     8161 tgctgccgat caattaccgc tgtttgacga actgtgtaag cgtgagcgcg caccctacgc
     8221 ggtgattggt gaagcgaccg aagaactgca tctttctctg cacgatcgtc attttgataa
     8281 tcagccgatc gatctgccgc tggacgtcct gcttggtaaa acgccgaaga tgacccgcga
     8341 tgtacaaacg ctgaaagcga agggcgatgc gctggtccgt gaagggatca ccattgcaga
     8401 cgcggtgaaa cgcgtactgc atctgccgac cgtggcagag aaaaccttcc tggtgaccat
     8461 cggcgaccgc agcgtaaccg gcatggtagc gcgcgatcag atggtggggc cgtggcaggt
     8521 gccggtcgct aactgcgcgg tcactaccgc cagcctcgac agctactacg gtgaagcgat
     8581 ggcgattggc gagcgtgcgc cggttgcact gctggatttc gccgcctctg cccgtctggc
     8641 ggtcggtgaa gcgttaacca atattgccgc aacacagatt ggcgatatca aacgcattaa
     8701 actttccgcc aactggatgg cggcggcagg ccaccctggt gaagatgcgg gcctgtatga
     8761 agccgttaaa gccgtgggcg aagagctttg tccggcgctg ggcctgacga tcccggtggg
     8821 taaagactct atgtcgatga aaacccgctg gcaggaaggt aacgaagagc gcgaaatgac
     8881 gtcgccgctg tcgctggtga tttctgcatt tgcccgcgtg gaagatgtgc gtcacaccat
     8941 cacgccacag ctttctaccg aagataacac actgctgctg attgatttgg gcaaaggcaa
     9001 taacgcgctg ggcgcaacgg cgctggcgca ggtttatcgt cagcttggcg acaaaccggc
     9061 agatgtacgc gacgtcgcgc aactgaaagg cttctatgac gcgattcagg cgctggttgc
     9121 acagcgtaag ctgctcgcat accacgaccg ctctgatggc ggcctgctgg taacgctggc
     9181 ggaaatggcc tttgctggtc attgcggcat taacgcggat attgcctctc tgggcgacga
     9241 tcgcctggcg gcgttgttta acgaagaact gggtgcggtg attcaggttc gtgccgctga
     9301 ccgtgaagcg gtcgagtccg tactggcaca gcatgggctt gctgattgtg tccattatgt
     9361 agggcaggcg gtttccggtg accgttttgt gattaccgcc aacgggcaga ctgtattcag
     9421 cgaaagccgc accacgttgc gtgtctggtg ggcagaaact acctggcaga tgcagcgcct
     9481 gcgtgacaac ccggagtgtg ctgaccagga gcaccaggcg aaatcaaacg acgccgatcc
     9541 gggcctgaat gtgaaactgt cgttcgatat caacgaagat gtggcagcac cgtatattgc
     9601 cactggcgca cgtccgaaag ttgctgtgct gcgtgagcag ggcgtgaact cgcatgttga
     9661 aatggcggca gctttccacc gtgcaggctt tgatgctatc gacgtgcata tgagtgacct
     9721 gctgaccgga cgcacgggcc tggaagattt ccacgccctg gtcgcgtgcg gtggtttctc
     9781 ctacggtgat gtgctgggtg ccggtgaagg ttgggcgaag tcaatcctgt tcaatgaccg
     9841 tgtacgcgat gagtttgcaa ccttcttcca ccgtccgcaa acgctggcgc tgggggtatg
     9901 taacggttgc cagatgatgt ctaacctgcg tgaactgatc ccggggagcg agttgtggcc
     9961 acgttttgtg cgtaacacct ccgatcgctt tgaagcgcgt ttcagcctgg ttgaagtaac
    10021 ccaaagcccg tctctgctgt tgcaggggat ggtgggctcg caaatgccga ttgctgtctc
    10081 tcatggtgaa gggcgcgtgg aagtgcgtga tgcggcgcat ctggcggcac tggaaagcaa
    10141 agggctggtg gcactgcgct atgtcgataa cttcggcaaa gtcactgaaa cctacccggc
    10201 taacccgaac ggttcaccga acggtattac ggcggttacg actgaaagtg gtcgagtcac
    10261 cattatgatg ccgcacccgg aacgtgtttt ccgtactgtc agcaactcct ggcatccgga
    10321 aaactggggc gaggatggcc catggatgcg cattttccgc aatgcgcgta agcagttggg
    10381 gtaagtcatc agttcattgg ttttgcagcc cggatgcggc attaagcacc aaccactatc
    10441 ataagctccc aactccggga gcttttttgt gtctgtaaat cacgacaatg ggtggtttac
    10501 cgtgtcgctt tctggcgaca cttaaatgat tgattttatt gcaatctaat aagtttatct
    10561 taagttgtct ctttttagcg acacagtggc tgatttacat tcaaatagcg aagaagccat
    10621 aaatccctca aagcaacgta taatcagtac gttaccaaac tattttcttt attggcacag
    10681 ttactgcata atagtaagca gtggctcatt caccgactta tgtcagcccc ttcgggacgt
    10741 gctacataaa attcgaatga cgcacaacaa ggtgcctgcc gtccaacttc tgatatcagc
    10801 gtagctatat caaccatcgg gcgaaacgtc gagttaggca ccgccttatt ccataacaaa
    10861 gccgggtaat tcccggcttt gttgtatctg aacttcccct cggttagcat caggctattc
    10921 gcgtctgacg agagtaacac cttgaaacgc tggcccgttt ttccccgctc attacgacaa
    10981 ctggtaatgc tggcattttt gctaattcta ctacccctgt tggtgctggc atggcaagcc
    11041 tggcaaagcc tgaatgcgct tagcgatcag gcggcgctgg ttaaccgcac tacgcttatt
    11101 gatgcccggc gtagtgaagc gatgaccaac gcggcgctgg agatggagcg tagctaccgt
    11161 cagtattgcg tgctggacga ccctacgctt gcgaaggttt atcaaagcca gcgcaagcgt
    11221 tacagcgaaa tgctcgatgc ccacgcaggc gtactgccag acgataaact ttaccaggca
    11281 ttgcgtcagg atttgaacaa tctggcccaa cttcagtgta acaacagtgg tcccgatgct
    11341 gccgccgccg cgcgtcttga agcctttgcc agtgccaata ccgaaatggt gcaggccacg
    11401 cgcacagtgg tgttctctcg tgggcagcaa cttcagcgtg aaatcgccga acgtgggcaa
    11461 tattttggtt ggcaatcgct ggtgctattt ctggtgagtc tggtgatggt gctacttttc
    11521 acgcggatga ttatcgggcc ggtgaaaaat atcgagcgca tgatcaaccg tctgggggaa
    11581 gggcgttctc tgggcaatag cgtttcgttc agtggaccga gcgagttacg ctcggttggg
    11641 caacgtattc tttggttaag tgagcgcctg tcatggctgg aatcccaacg ccatcaattt
    11701 ttaagacatt tatctcatga attaaaaacg ccactggcga gtatgcgcga gggcactgaa
    11761 ttactggctg accaggttgt cgggccgctt acgccagagc aaaaagaggt ggtgagcatt
    11821 cttgatagca gcagccgcaa tttgcaaaaa ctgatcgaac aactgcttga ttacaaccgt
    11881 aaacaggcgg acagtgcggt ggaactggag aatgttgaat tagcaccgct ggtggagaca
    11941 gtggtttctg ctcatagcct gcccgcacgg gctaaaatga tgcataccga cgtcgatctc
    12001 aaagcaacag cttgcctggc ggagccaatg ctgctgatga gcgtactgga taatctttac
    12061 tccaatgcgg tgcactacgg ggctgaatcc ggtaacattt gccttcgcag cagttcacat
    12121 ggtgcgcggg tttatattga tgtcatcaat acaggcacgc ccattccgca agaggaacgc
    12181 gccatgatct tcgaaccctt ttttcaggga agccaccagc gaaaaggggc ggtgaagggc
    12241 agcggtctgg gattaagcat tgccagggat tgtattcgcc gtatgcaagg ggaactgtat
    12301 ctggtcgacg agagcgggca agacgtttgt ttccgcattg aattaccgtc gtcgaaaaac
    12361 acgaaataat gctaacagcc tatcctatca ggctatttta tttgtcagtt cggccccggg
    12421 cagtgctcga aatcttcacg tacgatatgt acgctccggt ttctccgcgc tgtccatgtc
    12481 cagactgtct gcaacaatta cgcccactgg gataggcttt aagtctggtg aatatgcgac
    12541 acatttttca acgattattg ccccgacggc tgtggctggc agggctgcca tgtctggcat
    12601 tgctgggttg cgtgcagaat cacaataagc cagccattga tacgccagca gaagaaaaaa
    12661 ttccggttta tcaactggct gactatcttt ctactgaatg tagcgacatc tgggcgctgc
    12721 aaggtaaatc aaccgaaacc aatccgcttt actggctgcg ggcgatggat tgtgctgatc
    12781 gtttaatgcc tgcgcagtcg cgtcagcaag ctcgccaata tgacgacggc agctggcaaa
    12841 atacctttaa acaagggatt ttgctcgccg acgccaaaat tacgccgtat gagcgccgcc
    12901 agctggtcgc gcgtattgaa gcgttaagta ccgagatccc ggcacaggtt cgcccactgt
    12961 atcaactctg gcgcgacggt caggcgctgc aactgcaact ggcggaagag cgccaacgtt
    13021 acagtaaact ccagcagtcc agcgacagcg agctggatac attgcgccag caacatcacg
    13081 ttctacagca gcaactggaa ctcaccaccc gcaagctgga aaacctgacc gatattgaac
    13141 gccagctctc gacccgcaaa ccgactggaa atttctcgcc ggatacgccg cacgagagtg
    13201 aaaagcccgc gccatccacc catgaggtca ctcctgatga gccataaacc tgcgcattta
    13261 ttattggtcg atgacgatcc gggattgctg aaactgcttg gcctgcgcct gaccagcgaa
    13321 ggctacagtg tggtcacggc ggaaagtggc gctgaaggat tacgggtact gaatcgcgaa
    13381 aaagtagatt tagttatcag cgacctgcgg atggatgaaa tggacggtat gcagctattt
    13441 gctgaaatcc agaaagtgca gccgggaatg ccagtaatta ttcttactgc gcatggttct
    13501 atccccgatg ccgttgctgc aacacagcag ggcgttttta gtttcctcac caagcctgtc
    13561 gataaagatg cgctatatca ggcaattgac gatgcgctgg agcaatccgc gccagccacc
    13621 gatgaacgct ggcgcgaggc aattgtcacc cgcagcccgc tgatgctgcg tttgctggaa
    13681 caggcgcggc tggtggcgca atcagacgtc agcgttttga ttaacggtca gagcggcacc
    13741 gggaaagaga ttttcgccca ggctatccac aacgccagcc cgcgcaacag caaaccattt
    13801 attgctatta actgtggcgc attgcccgag caattgctgg agtcggagct gtttggtcat
    13861 gcgcgtggcg cgtttactgg cgctgtcagc aatcgcgaag gtttattcca ggcggcggaa
    13921 ggcggaacgc tatttctcga tgaaattggc gatatgcctg cgccgttaca ggtcaaactg
    13981 ctgcgcgtgt tgcaggagcg taaagtgcgc ccgctgggca gtaaccgcga tattgatatc
    14041 aatgtgcgga ttatttctgc cactcaccgt gatctgccaa aagcgatggc gcgcggggaa
    14101 ttccgcgaag atctctatta ccgcctcaac gttgtcagcc tgaaaattcc ggcactggcg
    14161 gagcgcacag aagacattcc gctactggca aatcacctgt tgcgccaggc ggcagagcga
    14221 cataaaccgt ttgtccgcgc gttctctacc gatgcgatga aacgcctgat gaccgcgagc
    14281 tggccgggta atgtgcgcca gttggtcaac gtgattgaac agtgcgtggc gctgacctca
    14341 tctccggtga ttagtgatgc gctggtggag caggcgctgg agggtgaaaa tacggcgctg
    14401 ccaacctttg ttgaggcacg taatcagttt gaactcaact atttgcgtaa gctgctgcaa
    14461 atcaccaaag gcaacgtcac ccacgcggcg agaatggcgg ggcgcaaccg gacagaattt
    14521 tataaactgc tttcccgaca cgagctggat gcaaacgatt tcaaggaatg aattggcgtt
    14581 atgtgttacg tttagcagat caaaagacag gcgacctttt caaggaatag catgaaaaag
    14641 attgatgcga ttataaaacc cttcaagctg gacgatgtcc gcgaagcact ggccgaagtc
    14701 ggtattaccg gcatgacggt gaccgaagtg aaaggctttg gtcgccagaa aggccatacc
    14761 gagctgtacc gcggcgcgga gtatatggtg gattttctgc cgaaagtgaa aattgagatt
    14821 gtcgtaccgg acgacattgt cgatacctgt gtcgatacca ttattcgcac ggcgcaaacc
    14881 ggcaaaatcg gtgacggtaa aatcttcgtc tttgacgtgg cacgggtcat tcgcatccgt
    14941 accggtgaag aggacgacgc ggcaatttaa accgcgtcaa gggttgccgg atgtttccat
    15001 ccggcaacat caaattacag caccttatgc gggccaaagc attcgtaatg aatgttttcc
    15061 tgcttcacgc ccagatccac taactgtttc gcggtaaact gcatgaagcc aaccgggccg
    15121 catagataga actgcattgt cggatcgctg aacgcacctt ccagtttgct caaatccatc
    15181 agaccttcgc tatcaaactg acctttagcg cgatcggctt cgctcggctg acgatgccag
    15241 gtgtgcgcgg taaagcgcgg cagtgactgc cccagttcct taacttcatc ggcaaaggcg
    15301 tgaacatcgc cattttctgc cgcatggaac cagttcactt gtgctgtgtg gcctgctttt
    15361 gccagcgtgt cgagcattgc cagcattggc gtttgaccaa caccggcaga gattaacgtc
    15421 actggtgtgt catctgcgac agccataaag aaatcacctg ccggagcgac cagtttcacg
    15481 acatcgccaa cattggcgtg attgtgcaac cagttggata cctgcccacc ctcttcgcgt
    15541 ttcaccgcaa tacgatagcc tttgccatcc ggtttgcgag tcaaagagta ctgacgaatt
    15601 tcctgatgcg ggaaaccttc cggcttcagc cagacgccga gatattgccc cggacggtat
    15661 tctgccactg cgccaccgtc gaccggctcc agttcgaagc tggtgataag cgcgctgcgc
    15721 ggtgttttag ccacaatgcg gaaatcgcga gtaccttccc aaccaccggc tttgctggcg
    15781 ttttcgttat agatttccgc ctcgcgattg ataaatacat tagccagtac accataggct
    15841 ttaccccacg cgtccagcac ttcctgcccc gggctgaaca tttcgtccag cgttgccaac
    15901 aggtgttcac cgacgatgtt gtactgttcc ggtttgatct ggaagctggt gtgcttctgc
    15961 gcgatttttt ctaccgctgg cagcagcgca ggcaggtttt caatattact ggcgtaggcg
    16021 gcaatagcgt taaacagggc ttcacgttga tcgccattac gctggttact catgttaaaa
    16081 atttctttga gttctgggtt atgagtaaac atacggtcgt agaaatgggc ggttaacttt
    16141 ggccccgttt ccaccagtaa agggatggtg gcttttactg tagcgatggt ttgagcgtca
    16201 agcatatggt cttccttttt ttgcatctta attgatgtat ctcaaatgca tcttataaaa
    16261 aatagccctg caatgtaaat ggttctttgg tgtttttcag aaagaatgtg atgaagtgaa
    16321 aaatttgcat cacaaacctg aaaagaaatc cgtttccggt tgcaagctct ttattctcca
    16381 aagccttgcg tagcctgaag gtaatcgttt gcgtaaattc ctttgtcaag acctgttatc
    16441 gcacaatgat tcggttatac tgttcgccgt tgtccaacag gaccgcctat aaaggccaaa
    16501 aattttattg ttagctgagt caggagatgc ggatgttaaa gcgtgaaatg aacattgccg
    16561 attatgatgc cgaactgtgg caggctatgg agcaggaaaa agtacgtcag gaagagcaca
    16621 tcgaactgat cgcctccgaa aactacacca gcccgcgcgt aatgcaggcg cagggttctc
    16681 agctgaccaa caaatatgct gaaggttatc cgggcaaacg ctactacggc ggttgcgagt
    16741 atgttgatat cgttgaacaa ctggcgatcg atcgtgcgaa agaactgttc ggcgctgact
    16801 acgctaacgt ccagccgcac tccggctccc aggctaactt tgcggtctac accgcgctgc
    16861 tggaaccagg tgataccgtt ctgggtatga acctggcgca tggcggtcac ctgactcacg
    16921 gttctccggt taacttctcc ggtaaactgt acaacatcgt tccttacggt atcgatgcta
    16981 ccggtcatat cgactacgcc gatctggaaa aacaagccaa agaacacaag ccgaaaatga
    17041 ttatcggtgg tttctctgca tattccggcg tggttgactg ggcgaaaatg cgtgaaatcg
    17101 ctgacagcat cggtgcttac ctgttcgttg atatggcgca cgttgcgggc ctggttgctg
    17161 ctggcgtcta cccgaacccg gttcctcatg ctcacgttgt tactaccacc actcacaaaa
    17221 ccctggcggg tccgcgcggc ggcctgatcc tggcgaaagg tggtagcgaa gagctgtaca
    17281 aaaaactgaa ctctgccgtt ttccctggtg gtcagggcgg tccgttgatg cacgtaatcg
    17341 ccggtaaagc ggttgctctg aaagaagcga tggagcctga gttcaaaact taccagcagc
    17401 aggtcgcgaa aaacgctaaa gcgatggtag aagtgttcct cgagcgcggc tacaaagtgg
    17461 tttccggcgg cactgataac cacctgttcc tggttgatct ggttgataaa aacctgaccg
    17521 gtaaagaagc agacgccgct ctgggccgtg ctaacatcac cgtcaacaaa aacagcgtac
    17581 cgaacgatcc gaagagcccg tttgtgacct ccggtattcg cgtgggtact ccggcgatta
    17641 cgcgtcgcgg cttcaaagaa gcagaagcga aagaactggc tggctggatg tgtgacgtgc
    17701 tggacagcat caatgatgaa gccgttatcg agcgcatcaa aggtaaagtt ctcgacatct
    17761 gcgcacgttt cccggtttac gcataagcga aacggtgatt tgctgtcaat gtgctcgttg
    17821 ttcatgccgg atgcggcgtg aacgccttat ccggcctaca aaactttgca aattcaatat
    17881 attgcaatat ccgtgtaggc ctgataagcg tagcgcatca ggcaatctta ggtatgtgat
    17941 catcaaggct tccttcggga agcctttcta cgttattgcg ccatcaaatc tgtcgtaact
    18001 gcgcctcaac atacaaatag ccaatcccca acacctgttg tgcgcggctt aattgcccaa
    18061 agccaatttg cgttgctttc acgctcggcc cttcgtcgcg gtcgaacggg ttaaatcccg
    18121 tctggcgaat aatggtatta agccagtgct caccaaaggc acaactgcga ccgtacaacc
    18181 aaatctgatt aatattgagg atgttgagga agttatacag actcaaacca atggcattgg
    18241 cactgcgatc aacccaactg gtgatccacg gttctccact ctaccaggca gcgattaact
    18301 gcgctgtagt cagtttttcc ggatcaagct gagtattaac cggttgtgat tttagccata
    18361 cccgcgcctg tttttttaat gcacttaacg aggcgacggt ttccaggcag ccgtaacgtc
    18421 cacagtcgca gacgacgcca tccggattaa cgatggtgtg actaatctgt ccgctaccat
    18481 acaaactgcc gcgataaatt tgctcgttaa tcacgaacga cgagccaatg ccgtaatcaa
    18541 cgttgatcac gcagaaatcc cgtttctgcg aattattttg ccatttctcc gccagcgcca
    18601 gcatcacgca gtcattatcg accatcaccc gaatgccgag cttctcttcc agcagatact
    18661 tcacctcaac cggcgttgtc cacggcgctt gcggcatggt ttgcgacacg ccggtcaccg
    18721 gatcaacctg accgtggatt gccagcgcca gattgatggt gcggtccggc cacaatttac
    18781 ggtggcgatg ccagcatttt tcgatttcgg acagcagcgc ctgcggcgtc ggtgcatcaa
    18841 tctgtaaata ctcaaattca cctttcggac ttaaacaagc attagcaacc tgacactcaa
    18901 tactggtggg cgtcacgttc aggcacagcg tccagtcacc ttccggcgca atcagccatg
    18961 taccgctact atgcccgcgc gtctggcttt catcgtcgat attcaccacc cgtttttcgc
    19021 tttccagttc ctgcaaaata ttactgactg ccggaatcga aatttgcgcc agccgggcca
    19081 gcgttgattt attggcgcgc ttttgccggt acagcagttc cagaatcacg catttgttat
    19141 ggtggcgaat ctgttgattg ttaatgcagg ctctcatctt cactaaacct tgtttagtta
    19201 attgcgtgat tattccgcga aaacattgcc agattgttca atacactgcc acaaatcttt
    19261 taattcagta tgtcttgtta atattgaggg caccatgact ccagtaaaag tgtggcaaga
    19321 gcgcgttgag atcccgacct atgaaaccgg gccgcaggat atacatccca tgttcctgga
    19381 aaatcgcgtt tatcagggat cgtccggcgc ggtttatccc tacggcgtga ccgatacgct
    19441 gagcgagcag aaaaccctga aatcctggca ggcggtgtgg ctggaaaacg actacatcaa
    19501 agtgatgatc ctgccggaac tgggcggtcg ggtgcatcgc gcgtgggata aagtgaaaca
    19561 gcgcgatttt gtttatcaca atgaagtcat taaacctgcg ctggtggggc tgctggggcc
    19621 gtggatttcc ggtgggattg agtttaactg gccgcaacat catcgcccga ccacctttat
    19681 gcccgttgat ttcaccctcg aagcccatga agacggtgca cagacggtgt gggtaggcga
    19741 aacggagccg atgcatggtt tacaggtgat gacaggtttc accctgcgcc ctgggcggcg
    19801 ctggaaatcg ccagccgcgt ctataacggc aacgccacgc cgcgtcattt cttgtggtgg
    19861 gccaacccgg cagtgaaagg gggtgaaggg catcagagcg ttttcccacc ggatttgact
    19921 gcggtgtttg atcacggcaa acgggccgtc tccgctttcc ccatcgctac cggcacttac
    19981 tacaaagtgg actactccgc tggagtggac atttctcgct ataaaaatgt gcccgttcca
    20041 acctcatata tggccgaaaa atcgcagtac gattttgtcg gcgcgtggtg tcacgatgaa
    20101 gatggcggtt tgctgcacgt tgccaaccac catattgcgc caggtaaaaa acagtggagt
    20161 tggggacaca gtgaatttgg ccaggcgtgg gataagagtc tgaccgacaa taacggcccg
    20221 tatatcgaac tgatgaccgg tatttttgcc gataaccagc ccgattttac ctggcttgat
    20281 gcttacgaag agaagcgttt tgagcagtat ttcctgcctt atcattctct gggcatggtg
    20341 caaaatgcct cccgcgatgc ggtgataaaa ctccagcgta gtgagcaggg gattgagtgg
    20401 gggctgtatg ccatctctcc gttgaacgga taccgcctgg cgatccacga aatcggcaaa
    20461 tgcaacgcgt tacttgatga tgccgtggca ctgatgcctg cgaccgccat ccagggcgtg
    20521 ttgcacggta tcaatcctga taggctgacc attgagcttt ccgatgccga cggcaatatt
    20581 gtactgagtt atcaggaaca tcagccgcaa gcgttgccgt taccggacgt cgccaaagcg
    20641 ccactggcag cacaagacat taccagtaca gatgaagcct ggtttatcgg tcagcatctg
    20701 gagcaatatc atcacgccag ccgttcaccg ttcgattact acctgcgcgg cgtggcgctg
    20761 gacccgctgg attatcgctg taacctggcg ctggcgatgc tggaatataa ccgcgcagat
    20821 ttcccgcaag cggtggcgta tgccagtcag gcgctgaaac gcgcacatgc gctgaacaaa
    20881 aacccgcagt gtggacaagc aagtttgatt cgcgccagtg cttacgaacg tcagggacaa
    20941 tatcaacaag ccgaagagga tttctggcgt gcggtctgga gcggcaacag caaagccggt
    21001 ggctattatg gcctggcacg actggcggcg cgtaatggta acttcgacgc tggtctggat
    21061 ttttgccaac aaagtcttcg cgcctgccca accaatcagg aagtgctttg cctgcataat
    21121 ctgctgctgg tgttaagtgg tcgtcaggac aacgcgcgtt tgcagcgcgc gaaactgctg
    21181 cgtgattatc cgctgaacgc cattctgtgg tggctgaact ggttcgatgg tcgtagcgaa
    21241 tcagccctcg cgcagtggcg cggtctgtgt cagggacgcg acgttaacgc cctgatgacc
    21301 gccgggcaac tgattaactg gggaatgccc actctggcga cagagatgct gaacgcactg
    21361 gactgtcagc gcacgttatc gctttatcta caagccagct tgctgccgaa agccgaacgc
    21421 ggcgaactgg tcgcaaaagc cattgatgtc ttcccgcagt ttgtccgttt cccgaatacg
    21481 ctggaagaag tggcggcgct ggagagtatt gaagagtgct ggtttgcccg tcatttactg
    21541 gcctgtttct actacaacaa gcgcagctac aacaaagcca ttactttatg gcaacgttgc
    21601 gtagagatgt cgccggagtt tgccgatggc tggcgcgggt tggcgatcca tgcgtggaat
    21661 aagcaacacg attatgaact ggccgcgcgt tatcttgata atgcttatca gcttgcgccg
    21721 caggatgcac gtctgctttt cgaacgggat ttgctggata agttaagtgg agccacaccg
    21781 gagaaacgac tggcgcgtct ggaaaataat ctggaaattg cgctgaaacg cgacgacatg
    21841 accgcagaac tgctcaattt gtggcatctc acgggtcagg cagacaaagc ggcggacatt
    21901 ctcgccacgc gcaaattcca cccgtgggaa ggcggggaag ggaaggtcac cagtcagttt
    21961 atcctcaacc agttattacg cgcctggcag catcttgatg ccagacagcc gcagcaggcc
    22021 tgcgaactgc ttcatgccgc gctgcattat ccggagaatt taagcgaagg ccgtttaccg
    22081 gggcaaactg ataacgacat ctggttctgg caggcgatat gcgccaacgc gcagggcgat
    22141 gaaactgaag cgacgcgttg tttacgtctg gcggcgaccg gcgatcgcac cattaacatc
    22201 cacagttatt acaacgatca gccggttgat tatctcttct ggcaaggaat ggcgctgcga
    22261 ctgctgggcg aacaacaaat cgcacagcaa ctgtttagtg aaatgaaaca gtgggcgcaa
    22321 gagatggcga aaaccagtat tgaggcggat ttctttgctg tttcacaacc tgacctgttg
    22381 tcgctgtatg gcgatttaca acagcagcat aaagaaaaat gcctgatggt ggcgatgctg
    22441 gcgtccgcgg gactcgggga ggttgcgcaa tatgaatctg ctcgcgctga attgacggcg
    22501 attaatccgg cctggccgaa agcggcatta ttcaccaccg tgatgccttt tatttttaac
    22561 tacgttcact aaaccctatt aactgtaata tttgagcggc acgcgctgcc gctcatcaca
    22621 aaataattag aataagccgt tgttaataaa aactctacag gaggcactat gcctaaaaaa
    22681 atgagaacaa cccgtaatct attattaatg gctacgctat taggtagcgc actatttgcc
    22741 agggctgcgg ataaagaaat gaccattggt gcaatatacc ttgataccct gggatattac
    22801 gctggagtgc gtcagggcgt tcaggatgcg gcaaaagatt cttcagtaca ggtacagtta
    22861 attgaaacta acgcccaggg tgatatttcg aaagaaagta cctttgttga taccctcgtg
    22921 gcgcgtaatg tcgatgccat tattttatcg gcagtgtctg aaaatggcag tagccgtacc
    22981 gttcgtcgcg ccagtgaagc gggtattccg gtgatttgct acaacacctg tattaatcaa
    23041 aagggtgtcg ataaatatat ctcggcgtat ctggtcggcg atccactgga atttggtaaa
    23101 aaactgggta acgctgccgc cgattatttt attgccaata aaattgacca gccgaaaatt
    23161 gccgtcatca attgcgaagc ctttgaagtt tgtgtgcagc gacgtaaagg atttgaagaa
    23221 gtattaaaat cccgcgttcc cggcgcgcaa attgtcgcta atcaggaagg gactgtttta
    23281 aataaagcga tttccgttgg tgaaaaactg attatctcca cgccggatct caacgccatt
    23341 atgggggagt caggcggtgc gacactcggc gcggtaaaag cggtacgtaa tcaaaatcag
    23401 gccggaaaaa ttgctgtttt cggttcggat atgacaaccg aaattgctca ggagctggaa
    23461 aacaatcagg tgctgaaagc ggtagtggat atttccggta agaaaatggg caatgctgtt
    23521 ttcgcgcaaa cattgaaggt tatcaataaa caagccgacg gtgaaaaagt gattcaggtg
    23581 ccaatcgatc tctataccaa aacggaagat ggtaaacagt ggctggcaac gcacgttgat
    23641 ggtctgccct aatcgctgat tcgcagaggt ccctatgttc acggcaacag aggcagtccc
    23701 ggtagcaaaa gtggtggcag gaaataagcg ttaccccggc gtcgttgcgc tggataacgt
    23761 taacttcacg ctcaataaag gcgaagttcg tgcgctgtta ggtaaaaacg gcgcgggcaa
    23821 atcgactctc attcgaatgc ttaccggcag cgaacgcccg gatagcggtg atatctggat
    23881 tggcgagacg cgactggaag gtgacgaagc tacgttgact cgccgtgccg ctgaactggg
    23941 ggttcgtgcg gtttatcagg aattaagtct ggtggaaggg ctgacggtgg cagaaaacct
    24001 ctgccttggt caatggcctc gccgcaacgg catgattgat tacctgcaaa tggcgcagga
    24061 tgcccaacgt tgcttacagg cgctgggcgt tgacgttagt cctgaacaac ttgtttcaac
    24121 gctaagcccg gcgcaaaagc aactggtgga aattgcacgg gtgatgaagg gcgagccgcg
    24181 cgtggtcatt cttgatgaac ccaccagctc gcttgccagt gcagaagttg aactggtgat
    24241 cagtgcggtg aaaaagatgt cggcactggg cgtggcggtg atttatgtca gccaccggat
    24301 ggaagaaatc cgccgcattg cctcctgtgc caccgttatg cgcgatggtc aggtggcggg
    24361 cgatgtgatg ctcgaaaaca cctccacgca tcatattgtg tcgctaatgc tagggcgtga
    24421 tcacgttgat attgcgccgg ttgcccctca ggaaattgtg gatcaggccg tactggaagt
    24481 ccgtgcgtta cgccataagc ccaagctgga ggatatcagc tttacgttac gtcgcggcga
    24541 agtgcttggc attgctggcc tgctgggggc agggcgcagt gaattgctga aggcgattgt
    24601 tgggctggag acgtatgaac agggcgaaat tgttatcaac ggcgagaaaa tcacgcgccc
    24661 cgattacggc gacatgctga aacgcggcat tggctatacg ccagaaaacc gcaaagaagc
    24721 ggggatcatt ccctggttgg gcgttgacga aaatacagtg ctgaccaatc ggcaaaaaat
    24781 cagcgccaac ggagtgctgc aatggtccac catccgccgc ctgaccgaag aggtgatgca
    24841 gcggatgacg gtcaaggccg ctagtagcga aacgcccatc ggcacgcttt ctggtggcaa
    24901 tcagcaaaaa gtggtgatcg gtcgttgggt ctatgccgcc agccagattt tgttgctcga
    24961 cgagccaacg cgcggcgtcg atatcgaagc caaacagcag atttaccgta ttgtccgtga
    25021 gctggctgcc gaaggaaaaa gcgtggtgtt tatctccagt gaagtggagg agttgccgct
    25081 ggtgtgtgac cgcatcctgt tattacagca cggcacgttc tcgcaggagt ttcactcgcc
    25141 ggtcaatgtg gatgagctga tgtccgccat tctgtctgtg cactgatgaa ttttaccgag
    25201 gaacctgaag atgtctgctt cgtcattacc attgccgcag ggcaagagcg tctcgctcaa
    25261 acaatttgtc agtcgccata ttaatgagat cggtttgctg gtggtgattg ccattcttta
    25321 tctggtcttc tccctgaacg cgcctggctt tatctcattg aataaccaga tgaacgtgct
    25381 gcgcgatgcc gccaccattg ggattgccgc ctgggcgatg acgctgatta ttatctccgg
    25441 tgaaattgat gtcagcgttg ggccgatggt ggcttttgtc tcggtgtgcc tggcattttt
    25501 gctgcaattt gaagttccgt tggcgattgc ttgtctgctg gtgttgctgt taggcgcgct
    25561 gatgggaacg ctcgccgggg tgctgcgcgg cgtgtttaac gtaccaagtt tcgttgccac
    25621 actggggctg tggagcgccc tgcgcggaat ggggctgttt atgaccaacg cactgccagt
    25681 gccgattgac gaaaacgagg tgctggactg gctgggcgga caatttctcg gtgtgccagt
    25741 atccgcgctg atcatgatgg tgttgtttgc gctgtttgtg ttcattagcc gaaaaaccgc
    25801 cttcgggcgc tcggtttttg ccgttggcgg taatgccacg gcggcgcagt tgtgcggtat
    25861 taacgttcgt cgggtgcgca ttcttatctt taccctttcg ggattattag cggcggtgac
    25921 cggcattttg ctggcggcac gcctcggttc cggtaacgca ggtgccgcaa acggtctgga
    25981 gtttgacgtc atcgccgcgg tcgtggtcgg cggtacggca ctttccggcg gtcgcggttc
    26041 cctgttcggt acattgcttg gtgtgctggt gattacgcta atcggtaacg gtctggtgct
    26101 gctcggtatt aactcctttt tccagcaggt ggtacgcggc gtcatcatcg tggtggcggt
    26161 gctggcgaat atcttgctga cccagcgaag cagtaaagcg aaacgctaaa ccttaacgcc
    26221 cctggcgcgg gtgcggcagg gatgaaaatt aatcgtattg ttaatgaggg atttatgaaa
    26281 acgatgctgg cagcttattt accaggaaat tcaaccgtcg atctgcggga agttgcggtg
    26341 ccgacgccgg ggattaacca ggtactgatc aaaatgaaat cctccgggat ttgcggaagc
    26401 gatgtccact atatctatca tcaacaccgt gccacggcgg cggcacccga taaaccgtta
    26461 taccagggat ttatcaacgg tcatgaaccg tgcgggcaga ttgtggcgat ggggcaaggc
    26521 tgccgccatt ttaaagaggg cgaccgcgtg ctggtgtatc acatttctgg ctgtggtttt
    26581 tgcccgaact gccgccgcgg ctttcctatc tcttgtactg gcaaaggaaa agcggcttac
    26641 ggctggcagc gtgacggcgg tcatgccgaa tatctgctgg cggaagaaaa agatctgatc
    26701 ctcctgccgg atgcgctgag ctacgaagat ggtgcgttta tcagttgcgg cgttggcacg
    26761 gcctatgaag ggattttgcg cggcgaagtt tccggcagcg ataacgtgct ggtggtcggt
    26821 ctggggccgg tcggcatgat ggcgatgatg ctggcgaaag gtcgcggtgc aaaacggatc
    26881 atcggcgttg atatgctgcc ggaacgtctg gcgatggcaa aacagttagg ggtgatggat
    26941 cacggctatt tagcaaccac cgaaggtctg ccgcagatta tcgccgaact cacccacggt
    27001 ggcgcggatg ttgcgctcga ttgttctggt aatgccgcag gtcgcttgct ggcactgcaa
    27061 tccaccgctg actggggacg ggtggtttac attggtgaaa ccggaaaagt ggaattcgag
    27121 gtcagcgccg atctgatgca ccatcaacgg cggattatcg gctcctgggt gaccagtctg
    27181 ttccatatgg aaaaatgcgc ccacgatttg actgactgga agctgtggcc gcgtaacgcc
    27241 attacccatc gcttctcgct ggaacaggca ggtgatgcct atgcgctgat ggcgagcggc
    27301 aaatgcggga aagttgtgat taacttcccg gattaaggag ttagcggatg accatctata
    27361 ccttatccca cgggtcgcta aagctggacg tttctgacca gggcggcgta atcgaaggct
    27421 tctggcgcga tacgacgccg ctcttacgtc ctggtaaaaa aagcggtgtg gcaaccgatg
    27481 cctcatgttt tccgctggtg ccatttgcaa accgggtgag cggcaaccgt tttgtctggc
    27541 aggggcgtga gtatcaactg caaccgaatg ttgagtggga tgcgcactat ctacacggcg
    27601 atggctggct gggcgagtgg caatgtgtct cgcacagcga tgatagtttg tgtctggtgt
    27661 atgaacatcg cagcggtgtc tatcactatc gggtaagtca ggcgtttcat ttaacggcgg
    27721 atacgctgac ggtgacgctc tctgtcacca atcaaggggc agagacgctg ccatttggta
    27781 ccggctggca tccttatttt ccgttgtcac cacaaacgcg gattcaggcg caggcgagcg
    27841 gttactggct ggaacgggag cagtggctgg cgggtgagtt ttgcgagcag ctaccgcagg
    27901 aactggattt taaccagctc gcgccgttgc cgcgccagtg ggtgaacaat ggttttgccg
    27961 gatggaatgg tcaggcccgc atcgagcagc cgcaggaagg ttatgccatc atcatggaaa
    28021 cgacgccacc tgcaccgtgt tatttcatct ttgtttccga ccctgcgttt gataaaggat
    28081 atgcgtttga tttcttctgt ctggaaccga tgagccatgc gccggatgat catcatcgcc
    28141 cggaaggggg tgatctcatt gcgctagcac caggggaatc aacaacttca gagatgtcgt
    28201 tgcgggtaga gtggctgtaa tttattaaat gccggataag gcgttcacgc cgcatccggc
    28261 atctttccta acgccgatcg agagaaattg cccccggccc ggtaattgcc aatagcaaga
    28321 acgcgccagc gatactgaca ttcttccaga aattaatcat atttggccca accgcatcgc
    28381 cggtcatatc ccagtaatgg tgaccaatca ccgccgtacc cagcgtgtag aaaataaaca
    28441 gcaccgccag cggacgggtg aaaaagccaa gcacgattaa tatcgcggcg ggcacttcca
    28501 taactaccgc aataatcgct gccagcatcg gcattggcgc gcccaacgag gccatatatt
    28561 ggaccgtacc gtcaaagccc atcattttgg gaaaaccaaa aataatgaaa attaagacca
    28621 cggcgatacg ggcaattaat aacaaaacgg ggcgggcagc tccaaaatca aaataacg
//
LOCUS       DAEEKQ010000036        28086 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.036, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000036 DAEEKQ010000000
VERSION     DAEEKQ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28086
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.036"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            54..1274
                     /locus_tag="KLK57_001538"
                     /pseudo
     CDS             54..1274
                     /locus_tag="KLK57_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
     gene            1247..1876
                     /locus_tag="KLK57_001539"
     CDS             1247..1876
                     /locus_tag="KLK57_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HBE0891935.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGFTQPIVVTHTDKNAMEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="KLK57_001540"
     CDS             complement(1877..3037)
                     /gene="ybdL"
                     /locus_tag="KLK57_001540"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HBE0891936.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNSSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHSQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3146..4234
                     /gene="hcxA"
                     /locus_tag="KLK57_001541"
     CDS             3146..4234
                     /gene="hcxA"
                     /locus_tag="KLK57_001541"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HBE0891937.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLNDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAVPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGDLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEMDINNHAEIDKVITHTLRP
                     VEYIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4244..4441)
                     /locus_tag="KLK57_001542"
     CDS             complement(4244..4441)
                     /locus_tag="KLK57_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBE0891938.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="KLK57_001543"
     CDS             complement(4623..6728)
                     /gene="cstA"
                     /locus_tag="KLK57_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HBE0891939.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFAITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKSALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(6909..7322)
                     /gene="entH"
                     /locus_tag="KLK57_001544"
     CDS             complement(6909..7322)
                     /gene="entH"
                     /locus_tag="KLK57_001544"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HBE0891940.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPMSEGKVRGVC
                     QPLHLGRQNQSWEIIVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7325..8071)
                     /gene="entA"
                     /locus_tag="KLK57_001545"
     CDS             complement(7325..8071)
                     /gene="entA"
                     /locus_tag="KLK57_001545"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HBE0891941.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFAQEQY
                     PFATEVMDVADAAQVAQVCQRLLAETERLDVLVNAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAVSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8071..8928)
                     /gene="entB"
                     /locus_tag="KLK57_001546"
     CDS             complement(8071..8928)
                     /gene="entB"
                     /locus_tag="KLK57_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HBE0891942.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEGLLPAPVPARKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(8942..10552)
                     /gene="entE"
                     /locus_tag="KLK57_001547"
     CDS             complement(8942..10552)
                     /gene="entE"
                     /locus_tag="KLK57_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HBE0891943.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLLNDSGE
                     HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTQQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARISAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10562..11737)
                     /gene="entC"
                     /locus_tag="KLK57_001548"
     CDS             complement(10562..11737)
                     /gene="entC"
                     /locus_tag="KLK57_001548"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HBE0891944.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QTSAHCFTRSQSLNVVERQAIPEQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12112..13077
                     /gene="fepB"
                     /locus_tag="KLK57_001549"
     CDS             12112..13077
                     /gene="fepB"
                     /locus_tag="KLK57_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HBE0891945.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYTLGTETFRLDYYSAMQVLDRLNSLLKKD"
     gene            complement(13158..14408)
                     /gene="entS"
                     /locus_tag="KLK57_001550"
     CDS             complement(13158..14408)
                     /gene="entS"
                     /locus_tag="KLK57_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HBE0891946.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14519..15523
                     /gene="fepD"
                     /locus_tag="KLK57_001551"
     CDS             14519..15523
                     /gene="fepD"
                     /locus_tag="KLK57_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HBE0891947.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFIGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15520..16512
                     /gene="fepG"
                     /locus_tag="KLK57_001552"
     CDS             15520..16512
                     /gene="fepG"
                     /locus_tag="KLK57_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HBE0891948.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16509..17324
                     /gene="fepC"
                     /locus_tag="KLK57_001553"
     CDS             16509..17324
                     /gene="fepC"
                     /locus_tag="KLK57_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HBE0891949.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATRITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="KLK57_001554"
     CDS             complement(17321..18454)
                     /gene="fepE"
                     /locus_tag="KLK57_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HBE0891950.1"
                     /translation="MSSLNIKQGSDAHFSEYPLASPSNNEIDLLNLIEVLWRAKKTVM
                     AVVFAFACAGLLISFILPQKWTSSAVITPAEAIQWQDLEKTFTKLRVLDLDVNIDRGG
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSKEAQTVLSGYIDYISALVVKESIENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANVNDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
     gene            complement(18670..22551)
                     /gene="entF"
                     /locus_tag="KLK57_001555"
     CDS             complement(18670..22551)
                     /gene="entF"
                     /locus_tag="KLK57_001555"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HBE0891951.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFEQPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLPGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQSCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNMGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVEQAVTHACVINQAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGPIIRATLNR"
     gene            complement(22548..22766)
                     /gene="ybdZ"
                     /locus_tag="KLK57_001556"
     CDS             complement(22548..22766)
                     /gene="ybdZ"
                     /locus_tag="KLK57_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HBE0891952.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCLLSAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            complement(22769..23971)
                     /gene="fes"
                     /locus_tag="KLK57_001557"
     CDS             complement(22769..23971)
                     /gene="fes"
                     /locus_tag="KLK57_001557"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HBE0891953.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQV
                     PEIAAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRDGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            24214..26454
                     /gene="fepA"
                     /locus_tag="KLK57_001558"
     CDS             24214..26454
                     /gene="fepA"
                     /locus_tag="KLK57_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HBE0891954.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQNYSLTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYQQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSEMVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            26629..27249
                     /gene="entD"
                     /locus_tag="KLK57_001559"
     CDS             26629..27249
                     /gene="entD"
                     /locus_tag="KLK57_001559"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HBE0891955.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGTTALAVVSRQ
                     PIGIDIEEIFSVQTARELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASER
                     QTEAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(27371..27523)
                     /gene="hokE"
                     /locus_tag="KLK57_001560"
     CDS             complement(27371..27523)
                     /gene="hokE"
                     /locus_tag="KLK57_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBE0891956.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(27874..28026)
                     /gene="hokE"
                     /locus_tag="KLK57_001561"
     CDS             complement(27874..28026)
                     /gene="hokE"
                     /locus_tag="KLK57_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HBE0891957.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
BASE COUNT         6733 a         7862 c         7277 g         6214 t
ORIGIN      
        1 catcaccgtc ttatctcact gttcaccctg taacgttcag agagcgtctt cagatgtcta
       61 tttataaaat tcctcttccg ctcaatattc tggaagctgc acgagagcgt attacctgga
      121 cactcaacac cctaccccgc gtttgtgtct cattttctgg cggcaaaaac tctggtttaa
      181 tgctgcatct gacagccgaa cttgcccgac aaatgggcaa aaaaatctgc gttttgttta
      241 tcgactggga ggcgcaattc tcttgcacca ttaactatgt tcagtccctg cgcgagttgt
      301 acaccgatgt catcgaagag ttttactggg ttgcgctccc gcttacgacg caaaattccc
      361 tttcacaata ccaacccgaa tggcagtgct gggaacctga tgtcgaatgg gtgcgtcagc
      421 ccccgcaaga tgcgataacc gatcctgact ttttctgctt ttaccagcca ggcatgacct
      481 tcgaacaatt tgtacgtgag tttgccgaat ggttttcaca aaaacgtccg gtggcgatga
      541 tgatcggcat ccgtgcggat gagtcctaca accgttttgt cgccatcgcc agtttaaata
      601 aacaacgttt tgccgacgat aaaccatgga ctacggccgc accaggcggt catagctggt
      661 acatttaccc catttacgac tggaaagtgg ctgatatctg gacctggtat gcaaatcatc
      721 aaaatctctg caacccactg tataacctaa tgtatcaggc aggcgttcct ctgcgtcata
      781 tgcgaatttg agaacctttt ggcccggagc aacgacaagg attatggctc tatcacgtta
      841 tcgaaccgga tcgctgggct gctatgtgcg cacgagtcag cggggtaaaa agtggcggca
      901 tttacgccgg acatgataat catttctatg ggcatcggaa aatcctcaag ccagaacatt
      961 tagactggca agaatatgca ttattgctgc tcaatagcat gccggaaaaa acagctgagc
     1021 attaccgcaa taaaatcgcc atttatttgc actggtatca gaaaaaaggc atcgaggttc
     1081 cacaaaccca gcaaggggac attggcgcga aagatatccc ctcctggcgg cggatatgca
     1141 aagttttact caataacgat tactggtgtc gggcattatc atttagtcct acgaaagcga
     1201 agaactatca gcgttataac gaacggataa aaggaaaacg tcaggaatgg gggatactat
     1261 gcaacaacga ttaacgcagg atttaacgca atttctcgcc agcttgccgg aagatgatcg
     1321 catcaaagcg attaatgaaa tccgcatggc gatccatcag gttagcccct ttcgtgaaga
     1381 gccagtggat tgcgttctgt gggttaaaaa cagccaactt atgcctaatg attacaaccc
     1441 aaacaacgtg gcaccacccg aaaaaaaact gctgcaaaaa tccattgaaa tagatggttt
     1501 tacccaaccc attgtggtca cacatacgga taaaaatgcc atggaaatcg tggatggttt
     1561 tcaccgccat gaaattggta aaggctcatc atcattaaaa ttacgcttaa aaggttattt
     1621 gccggtgacc tgtctggaag gtacgcgtaa tcaacgcatc gccgcgacta ttcgtcataa
     1681 tcgcgctcgt ggacgccatc aaatcaccgc catgtcggaa atcgtccgtg aacttagcca
     1741 gttgggatgg gacgataata aaattggcaa agagctggga atggacagcg acgaagtgct
     1801 gcgcctgaag caaattaacg gcctgcaaga gctgtttgcc gaccgtcaat attcccgcgc
     1861 ctggacggtt aaataactaa agctggcgca ggcgttcagc tgctgccagc aacgtcgatt
     1921 ccttcttggc aaaacagaga cgaatcagtt tatgtgggaa gggatcggcg caaaacaccg
     1981 acagcggaat cgccgctacg ccgtgctcct gcgtcagcca ctggcaaaac tcaacatcat
     2041 ccagggtaga aaccgcgctg taatccacca gcaaaaagta tgtaccttca cacggtaaaa
     2101 tctccagccg gctttcattt aaggcattta ccagaatatc gcgcttctgg cgataaaagt
     2161 ccggtaacgc aagataatgc tcaggttctg cacgtagcat atcagcaagc gccagctgtg
     2221 ccggggtatt caccgaaaag gtcagatact gatgtacctt gcgaatttcg gcgctgatgg
     2281 gcgctggcgc aacacaataa cccactttcc agccggtcat atgataggtc ttgccaaatg
     2341 aagaaaccgc cactgcccgc tcacgcagct gcgaatgcgc cagcacactg gcatggccct
     2401 gttgtgaaaa gttgatgtgc tcgtagactt catcgctaat gacaaaaatc tcgtgcccgg
     2461 cgatcgcctg ccacaaagcg gcgaaatcag cctgctgcca gacagttgca ctggagttat
     2521 gcggagtgtt gaggatcacc agtctggtgc gctcgcttaa taatgcggca aattcctgcc
     2581 agtcaacgcg aaaatgcggt ggttgcagtg ccatacgctt cactattccc ccagaaagcg
     2641 cgatggcggg ggcgtaactg tcatagctgg gatcaaaaca aatcacttca tcgccattgc
     2701 gcaccagtgc ggtaatcgcc gcgtataacg cttccgtcgc ccctgccgtt acggtgatat
     2761 cgctatcggc atctggttga tagccataca aacgttccgt tttctgagca atcgcctcgc
     2821 gcaaggcctg cacgccggtc atgggcgcgt attggtttgc cccctgggca acgtggtacg
     2881 ccagccgctc ctgtaaatag cgcggaccat caaaatcagg aaagccttgc gacaggttaa
     2941 tcgcctggtg ttgctgcgcc agcgcgctca tctgggtgaa aatagtggtg ccaagttgtg
     3001 gaagtttgct ttgtggaatc agagggttat ttgtcatttt tattgtacct gacgaagttg
     3061 cgtgttgaag gcactataac acgatgctag tatttggcaa tcaagacgtt tagatgtcta
     3121 aataaaacaa taaggacaac acaacatgcc tcacaatcct atccgcgtgg tcgtcggccc
     3181 ggctaactac ttttcacatc caggaagttt caatcacctg aacgattttt tcactgatga
     3241 acaactttct cgcgcggtgt ggatctacgg caaacgcgcc attgctgcgg cgcaaaccaa
     3301 acttccgcca gcgtttggac ttccaggggc aaagcatatt ttgtttcgtg gtcattgcag
     3361 cgaaagcgat gtacagcaac tggcggctga gtccggtgac gatcgcagcg tggtgattgg
     3421 cgtcggcggc ggcgcactgc tcgacaccgc gaaagccctc gcccgccgtc tcggtctgcc
     3481 atttgttgcc gttccgacga tcgccgctac ctgcgccgcc tggacaccgc tctccgtctg
     3541 gtataacgat gccggacagg cgctgcatta tgagattttc gacgacgcca attttatggt
     3601 gctggtggaa ccagagatta tcctcaacgc gccgcaacaa tatctgctgg cggggatcgg
     3661 tgacacgctg gcgaaatggt atgaagcggt ggtgctggct ccgcaaccag aaacgttgcc
     3721 gctaaccgtg cgactgggga tcaataatgc gcaggccatt cgcgacgtat tgttaaacag
     3781 tagcgaacag gcgctgagcg atcagcaaaa tcaacagtta acgcaatcat tttgcgatgt
     3841 ggtggatgct attattgctg gtggtgggat ggtcggtgat ctgggcgatc gttttacgcg
     3901 tgtggcggcg gctcatgccg tgcataacgg tctgactgtc ctgccgcaaa ccgagaaatt
     3961 tctccacggc accaaagtcg cttatggcat tctggtacaa agcgccttgc taggtcagga
     4021 tgatgtgctg gcgcaattaa ccggggcgta tcagcgtttt catctgccga ccacgctggc
     4081 ggagctggaa atggatatca ataatcatgc ggagatcgac aaagtgatta cccacaccct
     4141 gcgtccggtg gaatacattc attacctgcc agtcacgctg acaccagata cgttgcgtgc
     4201 agcgttcgaa aaagtggaat cgtttaaagc ctgacgtgca aatttagcag cagcgcgcgc
     4261 cgccttttcc accgtagcgc gcgtcctgcc gctcacggaa aaactcttcg taggtcatcg
     4321 gcgtttgatc gggatggtta acccgcatat gttcgacata gttgtcgtaa tcaggcatac
     4381 caatcatcag cttcgccgcc tgacctaaat attttccggc ttttgccagt gaatcaaaca
     4441 tcttggcctc cgctaaccat gttgctgaaa gagtgccgga tgcggcgtga acgccttatc
     4501 cggcctacaa atgctatcaa cgcgctcggt cagtcccctc gccccatcgg ggagagggtt
     4561 agggtgagga ggaaccctcg gcactatgcc aacattcgcc aacatccccc ctcactctga
     4621 ctttagtgtg cgccttttgc ctgcgccacg atctcctcga cattttccgg cattggctca
     4681 tacggcgttt ctttcgccgt tggcttcgga tctttcaatg ccgccagtgc cgacttaatc
     4741 gagaacagcg ccagaaccac cacgaccacc ataaagaaga tggttaaccc ggcatccaga
     4801 cggttgttga acaccagttg cgccagctgt gactcagtat actgcgacgg aatattgccg
     4861 ctgtctatca ttgcctggaa cttattagca atggccagga agccgacttt cgcatccggg
     4921 ctaaacgctt tctgccagcc tgcggtcagg gtacaaatca gcagccaggc cgttggtacc
     4981 agcgccaccc aggcgtaacg ttgacgtttc atcttgaaca acaccacggc acagagcatc
     5041 agcgccatcc ctgccagcat ctggttggca ataccaaaca gcggccacag agtgttaatg
     5101 ccgcccagcg gatcgaccac gccctgatgg aggaagtagc cccacgccag tacgcacagc
     5161 gctgttgcca gcaggttagc aggcagtgaa tcggtccgct tcaggccagg agacaccacg
     5221 cccagcagat cctgcaacat aaagcgcgca gcacgcgtac ccgcatctac cgccgtcaga
     5281 ataaacagag cttcaaacag aatggcgaag tgataccaga acgccacatc catcatacca
     5341 cccagcgcac cgtgcaggat gtaagccatc cccaccgcca gcgtcggcgc accgcccgca
     5401 cgggaaatga tcgactgttc acccacttcg ctggcaatct ggttgagcgt atccggagta
     5461 atcgcaaagc cccagctact caccacctgc gcggcagaag cgaccacatc cgccgtccct
     5521 gccggagcca gcaccgccat cgggctgttc atggcgaagt acacgcccgg atcgatgata
     5581 caggcggaaa ccagtgccat aatcgccacg aaggattcca ttaacattcc accgtagccg
     5641 ataaagcacg cctgcccttc gttcgccagc atcttcggcg tggtgccgga agagatcagc
     5701 gcatggaagc ctgacaccgc gccacaggcg atggtgataa acaggaacgg gaacagatta
     5761 ccggtccata ccgggccagt gccatcgaca aatttggtca gcgcaggcat ggtcagcgtc
     5821 ggacgcataa tcaaaatgcc taccgccaga ccaacgatag tcccgatttt caggaaggta
     5881 gagaggtagt cacgcggggc cagcagtaac cacaccggca gcaccgccgc cacaaaaccg
     5941 tagcccacca gcatccaggt cagctgcacg ccggtaaagt caaagtacgg tgcccaggtc
     6001 gggctttctg ccacccagcc gccagagata atggcaaaaa taaggaacac caggccaatc
     6061 actgacactt cgccaatacg ccccggacgc agatagcgca ggtagattcc cataaaaatc
     6121 gccagcggaa tggtgaacgc aacggtatac gttccccacg ggctatgagt cagggctttc
     6181 accacgatca tcgccagtac tgcaaggata atgaccatga tcataaagca ggctaccagc
     6241 gcaatcaccc cggcagttgg ccccatctct tctttgacca gctcacccag cgagcgaccg
     6301 tcacggcgcg tagaaacaaa cagcaccatg aaatcctgca ccgcaccggc gagaaccacc
     6361 ccggcgagca gccagatcat ccccggcagg tagcccattt gcgctgccag caccggcccc
     6421 accagcggcc ctgctccggc aatggccgca aaatggtgac cgaacagcac tttcttgtcc
     6481 gtcggcacat agtccagccc gtcgttatgg cgcaccgctg gcgtcatacg cgtcgggtca
     6541 accgccagca catttttggc gatatacagc ccataaaaac ggtaagcgat cagatagata
     6601 cagaccgacg ccaccacaat ccacagcgcg ttgatctgtt ccccacgatt taaagcaatg
     6661 tatcccagag caaatgctcc cattacagag agcactgtcc agacgaggta tttccctgat
     6721 ttgttcatag ttgttatccg tgtgcgtgtc catagagatg ttacattttg tttctataac
     6781 atctttgtac gatttaacaa cacggtaacc atgtaaatcg gagttgaaac caatatttaa
     6841 cttaacaatg ttaactcgat cactccgtta accgagtttt cctgcaatct catttaattc
     6901 tgtcgggctc atcccaaaac tgccgtaccc agccgacaag tgcagcaacg ccgcccctgt
     6961 tcatcgaaaa cgatgatttc ccagctctga ttttgccgcc caagatgcag cggctggcag
     7021 acgccgcgta ccttgccttc agacatcggg cgatgatggg tggcgttaag ttccgtaccc
     7081 accacacact gcccgtcacg ggtcatcata aatccggcca tcgatcccag cgtttcagcc
     7141 agcgccgccg acgcgccgcc atgcagcagg ccaaacggct gatgagtacg ggtatcaacc
     7201 ggcatttcgg cttccagcac atcatctccc agacgggtat acacaattcc cagatgcgcc
     7261 accattgtgt tatcgctggt ggcgttcagt tcgtcgagcg ttaaatggcg tttccagatc
     7321 atgcttatgc ccccagcgtt gagccgccat cgaccacaat atcctgtagg gtgatatggc
     7381 tggcgaggtc agaggcgagg aacaaaatcg tgttggcgat ctcttgtgga cgggcgattt
     7441 tccccagcgg aatgccgagt ttaaactgct cgccaaagcc gcgaatacgc tgttcttcgg
     7501 cgtcatcgct cacccacagc gtgcgttgca tatcggtgtc ggtggagcca ggcgaaacca
     7561 cattacagcg cacgccgcta accgccagtt ccagcccgac gctcaacgcc aggcttttca
     7621 gcgccgcttt cgatgcgcca taagcactca taccaatacg cggcgtgtgt gcggcgtcgg
     7681 acgccacagt gacaatcgcc ccgccccgct gacggcgaaa ctggttcatg gtttgctgaa
     7741 acaggttaaa cgcaccgccg acgttaaccg caaaagtctg ctgccagtcc tctttactga
     7801 gctgatcggt cgcgcccatg cgcaaaattc ccgccgcatt gaccagcacg tccagtcgct
     7861 ccgtttcagc taacagtcgc tgacatacct gcgcgacctg cgcagcatcg gcaacatcca
     7921 tcacttcggt cgcaaaggga tattgctcct gagcgaacgc ttgatcaaaa ccggtaactt
     7981 tcgctcccgc ttcaacaaac gccagcgccg tggcgtagcc gataccttta cctgcgccgg
     8041 ttacccagac atttttaccg ctgaaatcca ttatttcacc tcgcgggaga gtagcttcca
     8101 ccaggcgtcg atggtcgggt ttttcgccag catgacaaag tcgatatcgc catgcacttt
     8161 gcgccagcgc gccgccagcg ccatcatgcg caccgaatcc agaccgtagt cgatcaggtt
     8221 gtcgtcatcg aacggttcat cggactcgtc cagcaacggc aggataacct cacgcagcgc
     8281 cgctttcctg gcggggacag gtgctggcag taatccttca gtcatcacca cccggccaga
     8341 ccgcccggcc acatatttca gcgacatcaa atgctcgtca cggctgaaat cggccagcgc
     8401 atccgccacc ataaacggtt taatatcgcg cataaatgcg tcggttgcgg tggtcataca
     8461 gccaatgtgg gcatataccc cggtaataat cagctggtta cgtccactct ctttgagcat
     8521 ttgctccagc ggagaacgat gaaacgcgct gtagcgccac ttcaccagca ccgtgtcgtc
     8581 ggcatctggc gtcaggcgat ccaccacctt ttgctgttcc ggcgaacggg tcaggcccgg
     8641 cccccacata tcattcaaca gcgcccgatc ttcatcgctc tgctctttcg gctgggcggt
     8701 gtaataaacc gggatattgt gctgtttgca gtagtcgcgc agcgcagcaa tattcgcgat
     8761 cacctgctcc atcatcgggc agttctcgcc ccagaagctg acaaaatagt cctgcatatc
     8821 atggattaac aacgcggcac gttgcggttc aaaggcccag tcaactttat tctgcggaat
     8881 atcgtgagac tccggcagtg cgtaagcctg taattttgga atagccatcg tgttctctcc
     8941 ttcaggctga tgcgcgtgac gccagccact gacgtaattg ttttttatcg actttcccga
     9001 ccgccgtcag cggaagtgaa tccacacact ccacgcgatc cggtaattta aattcggcaa
     9061 taccctgttc acgcaggaaa cgacgcacct gcaccgcgcg cagcggctct tttaccacca
     9121 gataagcaca gcttttttcg cccatcagct catcttccat gctcaccagt gcggcataga
     9181 tcaccgccgg atggcgcagc agcaggtttt cgatctcttc ggcagcgatc ttctcgccgc
     9241 cacggttgat ctgatctttc tcgcgcccct gcacggtgat gtaaccctct ggatcaatag
     9301 agatcagatc gccggaacag taaaaaccgt tggcatcaaa ggcactggca ttgtgctgcg
     9361 gacttttgta ataaccgcgg aaggtgtacg gcccgcgcgt catcaggcgt ccaacttccc
     9421 cttgcggcag tggatttcct tcagcatcag caacccatac ttcatcgtcc ggacacattg
     9481 ggtaaccctg ggtatggata attttctccg cgctatcatc aagtcgggtg tagttcacca
     9541 gcccttccgc catgccaaac acctgctgca actggcagcc aatctcagcg gaaatacgcg
     9601 ccgcaagggt ggcagaaaga cgtgcgccgc cgacctgtaa cagtttcagc gaggcaagct
     9661 gcgcccggct ttcgccttca gccagcgcct gcaaccacag gctaactgcc ggcggcacca
     9721 gcgcggtaac gttaacctga tgttttgcaa tcaacgggaa gcaaagcgtg gcgctgggat
     9781 cggccgccag aacaaccgtt cctccggcaa gaaagacgcc cagcgatcct ggcgaactca
     9841 tagcgtagtt atgagccgcc gggatcgcgc acaggtagcg tgtctgttgt gtgaactgac
     9901 aaatctcgac gctacgacgc acgctgtagt agtagtcgtt atgagtgcgc gggatcagct
     9961 tcggtgtacc ggtggtgccg ccggaaagct ggaaataggc cacttcatca gcaggtgatg
    10021 gcgtagcagt aaaaccgtct gccggatggt taatcgcatc ctgcaagtta tgctcaccgc
    10081 tgtcgttgag cagttgcacc acgcgaatgg aggaatgttc tgtgacgaaa gtattgagga
    10141 aatcatcccc gctaaacagc gcatgttggc gatcggcaat cagtaacgcc ggttcaatct
    10201 ggctggcata ggcgttcagt tcactacgct gatggctgaa caacgccagc accggcgcaa
    10261 cgcccagttt cagcagtgcg aaaaaggtaa tgtaaagttc agcgacgtta cccagttgta
    10321 ccagcgcggt ttcaccgggt ttaatgccct gacggcgtaa actacacgcg aggttatccg
    10381 ccgcctgatt cagctcccgg taactcaact gtcgctcgcc gtcgataacc gcgatgctgt
    10441 cactcgcagc gtggcgagtc agaatgtcgg tcagcggcaa atcctgccag tagccttttt
    10501 cccgatagcg acgggcaaac tcttccggcc agcgggtgaa tggaatgctc atcctcgctc
    10561 cttaatgcaa tccaaaaacg ttcaacatgg tagaaagttt gacgcctgtt tcgcgccact
    10621 cacccaacgg tgacgacgca ggcacaatcc ccgctccggc aaacagacgc acctgatttt
    10681 cccgcagctt cgcgcagcgg atggtcacca cccattcgcc gttaccttcg ctgtcacacc
    10741 aacccacaat gccgccaaac agttcgcggt caaacggctc cagttcagca ataacctggg
    10801 tcgcggcctg atgcgggaaa ccgctcagcg cgggggtcgg atgcagcaga caggccagag
    10861 tcagtgcgtt ttcttgcgaa ttcgctttac cttcaaaggg agttgcgaga tgccacagcg
    10921 tcggcgtggt aatcaattgt ggagaggagg gaacgtgtaa ctcactactg cgttcgcgca
    10981 gtacctcttt catcgcctga gtcaccagtt catgttcatg gcgatctttt tctgacgcca
    11041 gcagacgatt acccgcttcg cgatcgagca cttcatccgg ctgacgacgc gcggaaccgg
    11101 ctaacggaat ggagctaaaa cgctcgccgt ctttacgtag cagcagttcc gggctggccc
    11161 ccagcaggac gccaccatca gccagcggga catggaagtt gtaactaacc gggttttgcg
    11221 caatcaaccg ttccagcaat acgccactat caatggcggc gtcagtggtg atatcaatca
    11281 atcgtgacaa caccactttg tcgacctgcg gcgtggcggt aagtgcggcg gcgcgggcaa
    11341 ccatctgttc aaacgtggtt tgttcaggaa ttgcctggcg ttccaccacg ttcagcgact
    11401 ggctgcgggt aaaacagtgg gctgaggtct gtttttcctg acgggagaac gactgccagg
    11461 attcgggaat atacagcgac gaaggctgac gtggatcgaa gggaatcgcc ccgaccatca
    11521 ccggattttt gatgccctgc gctttggcat cggcaaacag cgcggcgagt ttttgctgga
    11581 agggactgtc gggcgaatcc ccgttcacag ccggttcatc gaagcgggcg aaacatcctg
    11641 acgtcgtaaa actgcggtac ggcgacataa agaaaaagcg attgggcgca agtgttgcca
    11701 tggtctgctg tacttcctca gccagtgacg tatccatatc atcctccaca aaatgataaa
    11761 ggctttaata atgattatca tttatatttt cggtcgctaa cctaaaagca aacgcgcact
    11821 atgtcaactc ttgaggtaac gcaatctgca actcggggtt gcatctggtt gtggttacaa
    11881 tgaaaatgag aagcattatt gatgaattcg cataagcgca atgtgatgtc ctgcgccgtt
    11941 ttgccccctc tcccttccag ggtgagggct ggggtgaggg ttaatgttcg caccagtgct
    12001 ggctgttccc ctcaccctaa ccctctcccc aaaggggcga ggggacggat tgtgcgcttt
    12061 gtcgaatttg tcattacgcc cttaacctta ttaataacag gaagctgact tgtgagactc
    12121 gccccgctct accgcaacgc ccttctatta acaggacttt tgctttcagg aatagccgta
    12181 gttcaggccg ccgactggcc gcgtcagatt actgacagcc ggggcactca taccctggaa
    12241 agccagccgc agcgtattgt ttccaccagc gtcaccctga ccggatcact gctggcgatt
    12301 gatgctccgg tgatcgccag cggcgcgacc acgccgaata accgcgtcgc agatgaccag
    12361 ggctttttac gccagtggag caaggtggcg aaagaacgca aactgcaacg gctctatatc
    12421 ggcgaaccga gcgccgaagc cgttgccgcg caaatgccgg atctgatttt aattagcgca
    12481 accggcgggg attcggcgct ggcgctgtat gatcagcttt ccaccatcgc cccgacatta
    12541 atcatcaatt acgacgacaa aagctggcag tcgctgttaa cgcagcttgg cgaaattacc
    12601 gggcatgaga aacaagcggc agagcggatt gcacagtttg ataagcaact ggcagcggcg
    12661 aaagagcaaa tcaaattacc gccgcagccg gtcactgcca ttgtctatac cgccgctgca
    12721 cacagtgcca atctctggac gccggaatca gcacaagggc agatgctgga acaactcggc
    12781 tttacgctgg cgaagttgcc cgcagggtta aacgccagcc aaagccaggg caaacgccat
    12841 gacatcattc agcttggtgg ggaaaatctg gctgctgggt taaacggtga atcgctattc
    12901 ctgttcgccg gtgatcagaa agacgccgat gcgatttatg ccaatccact gctcgcgcac
    12961 ctgcctgcag tacaaaacaa gcaggtttat acgctgggaa ccgagacgtt ccgcctggat
    13021 tactacagcg ccatgcaagt gctggacaga cttaattcac tgttaaaaaa agattgataa
    13081 aacatacaat tagagcacta ataatcccct cgcccctttg gggagagggt tagggtgagg
    13141 ggaaaatgca cctttagcta actgtcagac gctgtcacct gcggcggcgt ctggcgaaaa
    13201 cgtcgcaact ccaccagcac cagcaataac aacacgccga taatcaacaa accaaaaccg
    13261 ctcgcgcttg cggaagcaac cggtgtcatc atcgcgccca aaccacccaa cagcgccgcg
    13321 cctatggcat cgcccgtcac gttctgcgcc gtccacaaac cgttaatccg ccctaacatc
    13381 gcttccgggg tttgcgtttg cagcattgta tattgcagca acgaactaac cgcgctcagc
    13441 cagccgaaca gcgccagaca aaccacgcct aaaatccaca tcggcatcag gccaaacaga
    13501 ccaatggcga ggaacgatcc cagcgtggag agcagcatca ataaccctgg tcgcgcacta
    13561 tgtgccagct tcccgctggt taacgcacca atagccgcac cgagcgggat cgccgcatag
    13621 agaaaaccaa tctgtgccgc cgacatctgc cagttgtcag ccagcgccgg atacagtacc
    13681 cgcaccgcgc tcgccatcgt caataaacca cccagcaacg caatccctcc taccagcggg
    13741 ctggcgagca gaaaacgaaa tcctgccagt aatgatttca acggatgctc gcgcggctgc
    13801 ggtggcggtg gcaacgccgg aaggcttaac aacggtagca aggtaataaa cgtgcccgcc
    13861 gccgccagcc cgtagttcca ggctacgccg ccggtcgcca gcaataaacc gccaatcatg
    13921 ggcgaaatca ccgaccccag acgcacggtc aacatggtga tcgccccggc ctgcattaag
    13981 ttttcacgcc ctaccagtgc aggtgttgcc gccagtagcg ccgtaacacc aagtgatgcg
    14041 aaaaaaccat cccataaacc aagtaaatag attgccagca atgacggctc cggcagcagc
    14101 gcgttcaggc acagtccaat gaagccaatg ccacaggtgc cgcgcgccag caaaatcact
    14161 tttttgcgct catagcgatc cgccagcaca ccgccaacca tcaggccaac aaacatcgcg
    14221 ccgccggtca gcgtcaccga aagccccacc tgccaggtag aatgcgtcat catctggatc
    14281 tgcaccggca ccgcgacgcc gagcaaaccc agagacacaa ttgagataaa acgagcgagg
    14341 aatactgcgc gaaacgccgg gtgcgttttc aacaggctga ggttaagcag ccaggattgt
    14401 ttattcatta caatgccttg ccatcaaata ttttaaatac ctattcttag gctgcacatg
    14461 ctaacatatc caaataagat cgataacgat aattaatttc attatcatgg aagttcgtat
    14521 gtctggttct gttgccgtga cacgcgccat tgccgtgccc ggattgctgt tattactgat
    14581 tatcgcgacg gcattaagcc tgctcattgg ggcaaaatca ctccccgcgt cagtagtgct
    14641 ggaggccttc tcaggcacct gccagagcgc cgactgcacc atcgtgctcg acgcgcgact
    14701 gccgcgcacc cttgccggtt tacttgcagg cggtgcgctt gggcttgctg gggcgttaat
    14761 gcaaaccctc acccgaaatc cacttgccga ccccggcttg cttggcgtga acgccggagc
    14821 cagctttgcc attgtgctgg gtgcggcgct gtttggttac tcatccgcgc aggaacaact
    14881 ggcgatggcc ttcgccgggg cgctggtggc ctcattgatt gttgccttta tcggcagtca
    14941 gggcggcggg cagttaagtc cggtgcgttt aaccctggcg ggcgtggcgc tggcggcggt
    15001 gctggaaggg ctgaccagcg gcatcgccct gcttaatcct gacgtctacg atcaactgcg
    15061 tttctggcaa gccggttcgc tggatattcg caatctacat accttaaaag tggtgctgat
    15121 cccggttctg atcgccggag caactgcgct attactgagt cgcgcgctga acagtttgag
    15181 cctcggcagt gacaccgcga cggcgctggg cagtcgcgtg gcgcgcacac agttgattgg
    15241 tctgctggcg attaccgtgc tttgtggtag tgcgacggcg gtagttggcc cgattgcctt
    15301 tatcggcctg atgatgccgc acatggcgcg ctggctggta ggtgccgatc atcgctggtc
    15361 gctgcccgtc acgctacttg ctacccctgc cctgctgctg tttgccgata tcatcgggcg
    15421 ggtgattgtg cccggcgaac tgcgcgtttc tgtggtcagt gcgtttattg gtgcaccggt
    15481 gctgatcttc ctcgtgcgac gtaaaacgcg aggtggtgca tgatttacgt ctctcgccga
    15541 ttactcatca cctgtttgct gctgatttcc gcctgtgtgg ttgcaggtat ctggggatta
    15601 cgcagcggtg ccgtcacgct ggaaatctcg caggtattcg ccgcgttgat gggcgatgcg
    15661 ccgcgcagta tgacgatggt ggtcaccgaa tggcgtttac cacgcgtgct gatggcgctg
    15721 ttgattggcg cggcgctggg cgtcagcggc gcgatttttc agtcgctgat gcgtaacccg
    15781 ctcggcagcc ctgacgtaat gggctttaac accggggcgt ggagcggcgt gctggtggcg
    15841 atggtgctgt ttggtcagga cctgacggct atcgcgctgg cagcaatggt gggcggcatt
    15901 gtcacttctc tgttggtctg gctgctcgcc tggcgtaacg gtatcgacac ctttcggttg
    15961 attattatcg gcatcggcgt tcgcgccatg ctggtggcct ttaatacttg gctgttgctg
    16021 aaagcgtctt tagaaacggc gctaacagcg ggtttgtgga atgccggatc gctcaatggc
    16081 ctgacgtggg caaaaacttc gccttccgca cccatcatca tattgatgct cattgccgcc
    16141 gccttgctgg tgcggcgaat gcgcttgctg gagatgggcg atgataccgc ctgcgcgctg
    16201 ggcgtcagcg tcgagcgttc gcgtctgtta atgatgctgg ttgcagtggt gcttaccgct
    16261 gcggcaacgg cgcttgccgg gccgatttcc tttattgctt tagtcgcgcc gcacattgcc
    16321 cgacgcatta gcggcaccgc tcgctggggg ctgacccagg cggcgctgtg cggagcgctg
    16381 ttactgctgg cggccgatct ctgcgcccaa caactgttta tgccgtatca acttccggtt
    16441 ggcgtcgtta ccgtcagcct cggcggtatt taccttatcg tcttgttaat tcaggagtct
    16501 cgcaaaaaat gaccgaatca gtagcccgtt tgcgcggcga acagttaacc ctgggatatg
    16561 gcaaatatac cgttgcggaa aatctgactg tagaaattcc tgatggtcac ttcacggcaa
    16621 ttatcggacc aaatggctgt ggtaaatcca cgttactgcg taccttaagc cgcctgatga
    16681 cgcctgctca cgggcatgtc tggctggatg gcgagcacat tcaacattac gccagtaaag
    16741 aagttgcacg ccggattggt ctgttggcgc aaaatgctac cacgccgggc gatatcaccg
    16801 tgcaggagct ggtggcgcgt ggacgttatc cgcatcaacc gctgtttacc cgctggcgca
    16861 aagaggatga agaagcggta acgaaagcga tgcaggccac gagaataact catctggcag
    16921 atcaaagcgt ggataccctt tctggcggac aacgccagcg agcgtggatc gcgatggtgc
    16981 tagcccagga aacggcaatt atgctgctcg acgaacccac gacctggctg gatatcagtc
    17041 atcagattga tctactggaa ctgttaagcg aactgaaccg cgagaaaggc tataccctgg
    17101 cggcggtgct gcacgatctt aatcaggcct gtcgttacgc cagccatttg attgcattgc
    17161 gggaagggaa aattgttgct cagggagcgc cgaaggagat tgtcactgct gaactgattg
    17221 agcgcattta tggtctgcga tgcatgatca ttgacgatcc ggtagccgga acgccgcttg
    17281 tggtgccgct cggacgaacg gcaccttcaa ccgcaaatag ttaaactaag tggtctgcca
    17341 tcatggcatc ctgttttctg gatgccatcg catagcgcaa taacacgcta ccacaagcca
    17401 ccatcccgcc gatcagcgcg gaaaggatca caataatcgc cttacccgga ccgtcttttt
    17461 tcactggcaa tgacgggctt aactgatatt taaacggcgt aaaattcaca tcgttgacat
    17521 ttgcttttgt taattgctcg acaagatact gccgattacg taattcaccg ttcagttccg
    17581 caacgtcagt aaccgctttt tcaatttcca gtttgcgttc aataccgtct gcgccgagag
    17641 aaatagagaa atcggggtca tctttaacgg cctgaccatt actgtatacg ggctttttaa
    17701 ttcctgccgc gttggcaatg tcgagtgaat aattgaggcg ctgaatgttt gcatcaagtt
    17761 gatttttcgt tttaatgcga tcctgagcca gtttttcttt ttcaaactgg gttttgatct
    17821 ccagtttatt acggacgttt tctatcgact ctttcaccac caacgcagag atataatcga
    17881 tatacccgct caaaacggtc tgcgcctctt tactggttgg cgcggtaaaa cttagcgtcc
    17941 aggaggtata cagtgacggt tcatcttttt tcttactggc attgtcatca accgctttca
    18001 ttttttcgct caatgcaaca atagcgcgat gcaaatccag ttcatcgatt ttcgcctctt
    18061 ttaattggtc catcacataa ggtgatgaac gcaggtactc ttccagcaag ctaacagact
    18121 gaaacttctt gataaacagg ttaaatgcac cgccacggtc gatattaaca tcgagatcaa
    18181 gaacgcggag tttggtaaag gttttctcca ggtcctgcca ctgaatggct tcggcgggag
    18241 taataacggc tgagctggtc catttttgcg gcaggatgaa agagatcagt aaacctgcgc
    18301 aggcaaatgc aaaaacgacc gccatgaccg tttttttggc ccgccataaa acttcgatta
    18361 gattaagtaa atcaatttca ttattactgg gcgacgccag aggatactca gaaaaatgag
    18421 cgtcacttcc ctgtttaata ttcaatgatg acatggggta taacctgaaa atgagtccaa
    18481 taggggaaaa tctccacggc gctcacttta ccagttggtg agaattatcc gaagctgaag
    18541 tttgtaaatt ccttccactg aatgcaatat tcactctatt taagagaaat attcatcaaa
    18601 cctataaatc aaacaaataa aaaacccata attattttct gtaattatgg gtttatagat
    18661 aatattaatt tacctgttta gcgttgcgcg aataatcggc ccaattttta caaacgcccc
    18721 tggagagata atatccacat gcgcacaatc ctgacgatag atatccagct ccgcaatcca
    18781 cggcgaccag gcgcgttcag gactcatacc ttcctgaagc gtgcgttcag caacaaacaa
    18841 cgtcgcttta ccgtcaaacg gtacgctatg agcagtcgtc agcaggcgca cagcatcagc
    18901 gtagttgcct tcaatggtgg taaacaactc cgttgaagta cttccctgct gtgccgccag
    18961 gaaggcctcg cgctcgcggt taatctccgc cagcacttcc gggtccagac cattagcttc
    19021 tttttcctgc cagttttgcg tttctggcgg ccaggtatcc agcaagccaa gaaatgccac
    19081 ctgttcgcca cgggcacgca gtcgcgccgc aataccctgc gccagcgtac cgccaaggga
    19141 ataccccagc aggtaataag ggccgtgcgg ttgttgctca agtaacgttg ccagatgcgc
    19201 ttcgcagact tcatccaggt ttgtcgctgt ctgcatgggt ccatgagggc gcggcgactg
    19261 aatgccaata atcgaccatt gtggatcgag ataacgcgag agtacgctaa actgccaggc
    19321 aaaaccggac gccggatgga aacaaaacag cgtcgggcca ttaccttcac gcaacggcag
    19381 aatggtttcg aatcccatgc gccgggagct gtcctcttca ccatcaataa tcgttgccag
    19441 tttggcgacg gttgacgcga ccatcacctg ccccggcgtc acctgacgag caaactgtcg
    19501 acttaactgc gctgccagtt tcattgccag tagcgaatga ccgcccagcg caaagaaatc
    19561 agcatcggca tcctgcacgt cacaacccag caacgacgcg aatgccgcgg cgataatcgt
    19621 ttcactgccc gctttcggtg cacgtcccga cgcttgtgtc ttcagttcag gcaacggtaa
    19681 ggctttgcga tccagcttgc cgttggcgct aagaggtaac tgtggaagtt gcagcagaac
    19741 caccggtacc atatgtggtg gcaatgtttc acgaagctgc gcctgcaatg cgctggtatc
    19801 caacggcagg cccgattgcg acaccagata gcccaccaac tgacgcgcat caccaccagt
    19861 ggctgccgcc tggttaatca cacaggcgtg ggtaacggct tgttcgacat ccggcagcgc
    19921 ctgcatcacg cgatcgattt cgcccagttc gatacgctgc ccgcgaattt ttagctgatc
    19981 gtcactgcgc ccgaggtact ccaccgcgcc gttatccagc cagcgggcaa cgtctccggt
    20041 acggtacatc cgttcgccag gagcaaaagg atcggcaata aagcggctgg cggtcagatc
    20101 ggggcgtccg agatagccct gcgccagttg aatgccggta agatagagat cacccgccac
    20161 acccggcggc accggatgca tcatcgcatc aagaatacgc agccccatgt tccacaccgg
    20221 ataaccaatc ggcacactgc tgccgcgcac ctgtgccagt tcctcgccaa aagccggata
    20281 ccagctgaca tctaccgccg cttccgtcgg gccatacaga ttatgtaacg gcgcaccggt
    20341 taactgttgc cattcacggc ataaatcagc cggtaaggct tcaccactac agaaaacctg
    20401 tttcaacgtc gcgcaactct ggcgagcggt ttgcggagtc agcgaggcaa caaatgccgc
    20461 cagcatcgac ggcacaaagt gcgtggtcgt tacgccatat tcggcaaaga attgctgcat
    20521 agcgagcggg tcacggtgcg cttccggttc agccatcacc agttttgccc ctgcgataaa
    20581 cggccagaaa aactcccaca ccgagacatc aaaactgcac ggcgtttttt gggcaacgac
    20641 atcttcgcct gtaagcgggt agtgattttg catccataac aggcggttaa cgatagccgt
    20701 ctgcccgacc attacccctt tcggcctgcc ggtggagcca gaggtaaaga tgatataagc
    20761 cgtgtggtgc ggttgtgaaa gttgcagcgg cgcactgccc tggggggtaa gcggggcgtt
    20821 atagcaaagg cttgttaaat tgggaacatc gctaaagcgc ggcagttgat cgtcggtggt
    20881 aattaacagc gacggacgcg catcttccag catcattttc aggcgatcgt ccggatagcc
    20941 ggtatccagc ggtagccagg ccgcgccagc ttcaactatt gcatggagtg ccagggtcaa
    21001 aaagaccgag cgcggtagtg ccaccgccac gctgtcccct ggtttaacgc cgcgctcacg
    21061 cagcagattc gccagcgcca ccacctgctc gcgcatttcc cgatagctga acaggtaacg
    21121 cgcatctgcc agcgccggag catccggtgt ttttgccgct tgttctgcca ccagcgcgct
    21181 cagcgtggtt tctggaatct caacctgagt ggcgttgatc tgcgccagct gcgcatactc
    21241 acctggcagc ataatatcga catcgccgca caacagcgcc ggatccgcgg cgaactgggc
    21301 aatcagcatt ttcaggcgtt cagcatgctg gattaacgtt ggctcatcgt aacgctgttt
    21361 attggcgagg atctcaatac tcaaatcacc gtgttcatcc gggaacaggg ccagttcaag
    21421 gtcattaacc ggaccggttg ccagggtatg ggtttgcgcc tgaacaccag gaatatccag
    21481 ttggtaatca aataccttaa tattgagtac cggaccaaac agcggttcat cacctgccgc
    21541 tcgcccgctg tcacggacaa tttgttcggc atcgtaacgt tgatggcgac gcattttttt
    21601 cagttgagcc gccagtcggg ttgccagttc cggcagcgtt tcttgcgccg caatgtgaat
    21661 acccaacggt aaaacgttga gcacgggtcc gctagccgtc agcgccgccg agcccagtcg
    21721 acgcataaag ataaatccgg cggcatagtc catgcgattg cacaatcgcc ccagccacaa
    21781 ggctgccagc gcaagggcta aatcggtacg ctgcacacct gaaagttgcg tagccagctg
    21841 gcggaattcc ccgtcggtaa attccagttt caggcgcaga atatctgccg aagcgctgcg
    21901 ccccggtaaa ggtgccggag aaagtgacgc gggcggcggc agttgacgac gctgttctgc
    21961 ccagaatgcc gcatcacgct gccaggcttc gctttcgcgg tattgctggt actcttccac
    22021 tacatcagcg aaaggcgtaa acggcgaagc aggcgttggt tcgccacgca gccatgtgca
    22081 gtaaatattg gcgatctggc gagtaatggc cgggaaactg aagccatcga ccagcaaatg
    22141 gtgataacgc tgataccagt accagcggtt atccgcaaca tgtatcagct gatgaaagac
    22201 cagtggttta ccgctatcga cgcgcaaatc ttgttgcaaa tccgccagca ttaatgcctg
    22261 cgcagtaccg tgcggatcaa tattggttcg caggtcgata atttctggct gttcgaatat
    22321 cagcgcatca tcgacccact gccagacttc gccgttatct tccgtaaaac gcatccgcaa
    22381 cgtatctgct tgcgctagtc cggcaaccac cgcgcgggcc agtaatggcg catcaacatc
    22441 tccagttaac tcaacgtaat gcgccacgct ccaggcggag ggtaaatccg acagtttttc
    22501 tgccatccag atgccgggct gtgcggcgac caaaggtaaa tgctggctca ttgtgcctcc
    22561 tgcaactggg tgaaattggc cggtgtcaga gtacgccagt ggacttccag ccactgctgg
    22621 caggacgcct gtgactgcgg ctgacacaca atgtcccagc ctgccgataa gaggcattgt
    22681 tgcggccaca gactgaattg cccctgcgca ttgcgcaaaa tgtaaaacgc tccctgcgga
    22741 tcatcgaagg gattactgaa tgccatactc aactcctgtc atggaaaagt ggttgccaga
    22801 ggtcgattaa cccctgcatc aagccaccgc gccaacaaag cgcatcatgt ccgccgtcaa
    22861 cctgacgcca gaaaatggat tcttttatag ggtgcaattg cgcatacagc gcctgattgg
    22921 cccgcatgat catcggctcg cgaatacccg cttccagcac aatgcgcaga ccttcggcgc
    22981 taacttcacc agcttttagc ttttcaagta acacgccctc ttgctgcccg tcccgatgcg
    23041 gccaccagta cgatcctgac tggcttaata cacagccaaa gcgttcaggc cagtgcagtc
    23101 cggcatacag cgcggacagc ccaccaaaac tctgcccggc gaccacggtg cgatcggcgc
    23161 gatcgctaaa aggggcaata gctttcacca ggggtaataa ctcttgctgt actgccagcc
    23221 agaaatccgc attacacggc agttcgtggg cgcggtgcgt ggtgtcgata gcgtcgatca
    23281 acacatacac ggcgggagga agttgctgac gatgggtcag cgaagtcagc acaggccaga
    23341 cgggcatact ttgcgcccaa aattcgccat cgagcaaaac tgccagcgga cgttcttctg
    23401 ctgttacatc gccggtggta aaaatccata cacgacgtga atttttcaac cgttcacttt
    23461 tccagataat ttctttggct gctatttctg gcacttgcgg acaatcccat cccggttgca
    23521 gaggcgcttg cggcatttcg agtgcagaaa cagcgtgccc tcgcccgcct ttccagcttt
    23581 gtggattcag cggatcggct attgcctggg gtaatagttt tcgccagcct tcacgtaatt
    23641 cgaggcgatc gggggatggt gcagaaaaaa tgtcatcgcg ttcggtggga ataaagcagt
    23701 agctgccgcg ccagttggca ttgagttgtg tcgtccactg ccagacatca gtacctgcaa
    23761 ttcgctgcat cgactggggc tggctgttct gatgatgatc ggtcacacca gtgatgtaga
    23821 tccatacgcg ctttatcgtc gagtattctt cagaaccttg gggatcacgc caccagaaag
    23881 tgacctcaaa catttcgtca ttcagacgct gccattccgg gccatgtttc gactgccacc
    23941 agctctcact tcctactttt aacgccgtca caccataacc ccatgtttac tgtgcaattt
    24001 ttcattgatt gcagaaatat attgataata ttattgataa ccatttgcat ttgcaatagc
    24061 gtaatggcgc gccgtgggaa gcgcggacat ttattaacca actgcactgc gtgtctttca
    24121 ggatcaaagg ttttcgcggt agcgggatgc gtcgtgttga ggacgaccat gcccgacagt
    24181 tgcaattcgt ggcaaaaatg caggaataaa acaatgaaca agaagattca ttccctggcc
    24241 ttgttggtca atctggggat ttatggggta gcgcaggcac aagagccgac cgatactcct
    24301 gtttcacatg acgataccat tgtcgttacc gccgccgagc agaacttaca ggcgcctggc
    24361 gtttcgacca tcaccgcaga tgaaatccgc aaaaaccccg ttgcccgcga tgtgtcggag
    24421 atcatccgta ccatgccagg cgttaacctg accggtaact ccaccagtgg tcagcgtggg
    24481 aataaccgac agattgatat tcgcggtatg ggtccggaaa acacgctgat tttgattgac
    24541 ggcaagccgg taagcagccg taactcggtg cgtcagggct ggcgtggcga gcgcgatacc
    24601 cgtggtgata cctcctgggt gccacctgaa atgattgaac gtattgaagt tctgcgtggt
    24661 ccggcagctg cgcgttatgg caacggcgcg gcgggcggcg tggttaacat cattaccaaa
    24721 aaaggcagcg gtgagtggca cggctcctgg gacgcttatt tcaatgcacc agaacataaa
    24781 gaggaaggtg ccactaaacg caccaacttc agcctgaccg gtccgctggg cgacgaattc
    24841 agcttccgct tgtatggcaa cctcgacaaa acccaggctg acgcgtggga tatcaaccag
    24901 ggccatcagt ccgcgcgtgc cggaacgtat gccacgacgt taccagccgg gcgcgaaggg
    24961 gtgatcaaca aagatattaa tggcgtggtg cgctgggatt tcgcgccatt gcaatcgctg
    25021 gaactggaag caggttacag ccgccagggc aacctgtatg cgggcgacac ccagaacacc
    25081 aactccgatt cctatacccg ctcgaaatat ggcgatgaaa ccaaccgtct gtatcgccag
    25141 aactactcgc tgacctggaa cggcggctgg gataacggag tgaccaccag caactgggtg
    25201 cagtatgaac acacccgtaa ctcgcgtatt ccggaaggtc tggcaggcgg taccgaaggg
    25261 aaatttaacg aaaaagcgac acaggatttc gtcgatatcg atcttgatga cgtgatgctg
    25321 cacagcgaag ttaacctgcc gattgatttc ctcgttaacc aaacgctgac gctgggtacg
    25381 gagtggaatc agcaacggat gaaggacttg agttccaata cccaggcgct gaccggggcg
    25441 aacaccggcg gcgctattga tggtgtgagt gccaccgacc gtagcccgta ttcaaaagca
    25501 gaaattttct cgctgtttgc cgaaaacaac atggagctga ctgacagcac catcgtaacg
    25561 ccggggctgc gtttcgatca tcacagtatt gtcggcaata actggagccc ggcgctgaac
    25621 atatcgcaag gtttaggcga tgacttcacg ctcaaaatgg gcattgcccg tgcttataaa
    25681 gcaccgagcc tgtaccagac taacccgaac tacattctct acagtaaagg tcagggctgc
    25741 tatgccagcg cgggcggctg ctatcagcaa ggtaacgatg acctgaaagc agaaaccagc
    25801 atcaacaaag agattggtct ggagttcaaa cgcgacggct ggctggcggg cgtcacctgg
    25861 ttccgtaacg attatcgcaa taagattgaa gcgggctatg tggctgtagg gcaaaacgca
    25921 gtcggcaccg atctctatca gtgggataac gtgccgaaag cagtggttga aggtctggaa
    25981 ggatcgttaa acgtaccagt tagcgaaatg gtgatgtgga ccaataacat cacttatatg
    26041 ctgaagagtg aaaacaaaac cacgggcgac cgtttgtcga tcatcccgga gtacacgttg
    26101 aactcaacgc tgagctggca ggcgcgggaa gatttgtcga tgcaaacgac cttcacctgg
    26161 tacggtaagc agcagccgaa aaaatacaac tacaaaggtc agccagcggt tggcccggaa
    26221 accaaagaaa tcagtcctta cagcattgtg ggtctgagcg cgacctggga tgtgacgaag
    26281 aatgtcagtc tgaccggcgg cgtggacaac ctgttcgaca aacgtttgtg gcgtgcgggt
    26341 aatgcccaga ccacgggcga tctggcaggg gccaactata tcgcgggtgc cggggcgtat
    26401 acctataacg agccgggacg tacgtggtac atgagcgtaa acactcactt ctgatgctaa
    26461 cgtcagattg ttgacaaagt gcgcgtcgtt catgccggat gcggcgtgaa cgccttatcc
    26521 ggcctacaaa aatcttgcca attcaatata ttgcaggacc atgtaggcct gataagcgta
    26581 gcgcatcagg cagttttgcg tttgtcatca gtctcgaata tggtcgatat gaaaactacg
    26641 catacctccc tcccctttgc cggacatacg ctgcattttg ttgagttcga tccggcgaat
    26701 ttttgtgagc aggatttact ctggctgccg cactacgcac aattgcaaca cgctggacgt
    26761 aaacgtaaaa cagagcattt agccggacgg atcgctgctg tttatgcctt acgggaatat
    26821 ggctataaat gtgtgcccgc aatcggcgag ctacgccaac ctgtctggcc tgcggaggta
    26881 tacggcagta ttagccactg tgggactacg gcattagccg tggtatctcg tcaaccgatt
    26941 ggcattgata tagaagaaat tttttctgta caaaccgcaa gagaattgac agacaatatt
    27001 attacaccag cagaacacga gcgactcgcg gactgtggtt tagccttttc tctggcgctg
    27061 acactggcat tttccgccaa agagagcgca tttaaggcat cggagagaca aactgaagca
    27121 ggttttctgg attatcagat aattagctgg aataaacagc aggtcatcat tcatcgtgag
    27181 aatgagatgt ttgctgtgca ctggcagata aaagaaaaaa tagtcataac tctgtgccaa
    27241 cacgattaat tgacaacatc tggtacgatt cgcccgcagc catcactgac tgcgggcgaa
    27301 agtgtaaagc aggtgcctta ccactctgac ctgacaaccg gatatgcagg gattgctccc
    27361 cgcacaacgg ctacttcttc ggttcgtaag cgagaacagc cttaaactca atattacgtt
    27421 cctttacggt gaactcacac agcgagtctc cgaccagaag cgtaaatccc agcaccgtca
    27481 aacacagcac tatgactgcc acaagggcat atttcgtcag catgtttata ttgcctccat
    27541 gcgaagaagg gactaccatc cgagttgttg aggttcggag tggcagcccc aggattgata
    27601 gaaatatctc ctggggcttc atctttccag acctcagagt aaacctgaaa ccggaaagct
    27661 tcaggcaccc gccgttatct tacccttcct cagcaaaata tctgtttatt tatacagtaa
    27721 gtcctgtttc ggacttctgt tggcaacact cgttattgct cgtaaaacga aatcgcccgc
    27781 agccatcact gaccgcgggc gaaagtgtaa agcaggtgcc ctaccatcct gacctgacaa
    27841 ccggatatgc ggggattact ccccgcacaa cggctacttc ttcggttcgt aagcgagaac
    27901 agccttaaac tcaatattac gttccttcac cgtaaactca cacagcgagt ctccgaccag
    27961 aagcgtaaat cccagcaccg tcaaacacag cactatgact gccgcaaggg catatttcgt
    28021 cagcatgttt atattgcctc catgcgaaga agggactacc atccaaattg ttcgggtttg
    28081 gagtgg
//
LOCUS       DAEEKQ010000037        28041 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.037, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000037 DAEEKQ010000000
VERSION     DAEEKQ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28041
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.037"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..133)
                     /locus_tag="KLK57_001562"
     CDS             complement(<1..133)
                     /locus_tag="KLK57_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0891958.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..572
                     /gene="tnpA"
                     /locus_tag="KLK57_001563"
                     /pseudo
     CDS             141..572
                     /gene="tnpA"
                     /locus_tag="KLK57_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(703..2026)
                     /locus_tag="KLK57_001564"
                     /pseudo
     CDS             complement(703..2026)
                     /locus_tag="KLK57_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-alpha-glucosidase"
     gene            complement(2026..3642)
                     /locus_tag="KLK57_001565"
     CDS             complement(2026..3642)
                     /locus_tag="KLK57_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HBE0891959.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQTGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFILGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            3938..4654
                     /locus_tag="KLK57_001566"
     CDS             3938..4654
                     /locus_tag="KLK57_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE0891960.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            complement(4651..6312)
                     /locus_tag="KLK57_001567"
     CDS             complement(4651..6312)
                     /locus_tag="KLK57_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HBE0891961.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKDTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGLLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(6508..6936)
                     /gene="ibpB"
                     /locus_tag="KLK57_001568"
     CDS             complement(6508..6936)
                     /gene="ibpB"
                     /locus_tag="KLK57_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HBE0891962.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(7048..7461)
                     /gene="ibpA"
                     /locus_tag="KLK57_001569"
     CDS             complement(7048..7461)
                     /gene="ibpA"
                     /locus_tag="KLK57_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HBE0891963.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(7455..7527)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            7767..8099
                     /locus_tag="KLK57_001570"
     CDS             7767..8099
                     /locus_tag="KLK57_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBE0891964.1"
                     /translation="MIRNVLLAFMICSGMALLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(8101..9315)
                     /locus_tag="KLK57_001571"
     CDS             complement(8101..9315)
                     /locus_tag="KLK57_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HBE0891965.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYCASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            9416..10480
                     /gene="cbrA"
                     /locus_tag="KLK57_001572"
     CDS             9416..10480
                     /gene="cbrA"
                     /locus_tag="KLK57_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HBE0891966.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQHGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRSVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKD"
     gene            complement(10477..11814)
                     /locus_tag="KLK57_001573"
     CDS             complement(10477..11814)
                     /locus_tag="KLK57_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0891967.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(11889..13037)
                     /gene="dgoD"
                     /locus_tag="KLK57_001574"
                     /pseudo
     CDS             complement(11889..13037)
                     /gene="dgoD"
                     /locus_tag="KLK57_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(13034..13651)
                     /gene="dgoA"
                     /locus_tag="KLK57_001575"
     CDS             complement(13034..13651)
                     /gene="dgoA"
                     /locus_tag="KLK57_001575"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HBE0891968.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(13635..14513)
                     /gene="dgoK"
                     /locus_tag="KLK57_001576"
     CDS             complement(13635..14513)
                     /gene="dgoK"
                     /locus_tag="KLK57_001576"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HBE0891969.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPAHFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFTAGLERGLNTPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVAHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(14510..15199)
                     /gene="dgoR"
                     /locus_tag="KLK57_001577"
     CDS             complement(14510..15199)
                     /gene="dgoR"
                     /locus_tag="KLK57_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HBE0891970.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            15168..15800
                     /locus_tag="KLK57_001578"
                     /pseudo
     CDS             15168..15800
                     /locus_tag="KLK57_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312658.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(15846..16658)
                     /gene="yidA"
                     /locus_tag="KLK57_001579"
     CDS             complement(15846..16658)
                     /gene="yidA"
                     /locus_tag="KLK57_001579"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HBE0891971.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(16773..17171)
                     /locus_tag="KLK57_001580"
     CDS             complement(16773..17171)
                     /locus_tag="KLK57_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HBE0891972.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            complement(17411..19825)
                     /gene="gyrB"
                     /locus_tag="KLK57_001581"
     CDS             complement(17411..19825)
                     /gene="gyrB"
                     /locus_tag="KLK57_001581"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HBE0891973.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(19854..20927)
                     /gene="recF"
                     /locus_tag="KLK57_001582"
     CDS             complement(19854..20927)
                     /gene="recF"
                     /locus_tag="KLK57_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HBE0891974.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(20927..22027)
                     /gene="dnaN"
                     /locus_tag="KLK57_001583"
     CDS             complement(20927..22027)
                     /gene="dnaN"
                     /locus_tag="KLK57_001583"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HBE0891975.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(22032..23435)
                     /gene="dnaA"
                     /locus_tag="KLK57_001584"
     CDS             complement(22032..23435)
                     /gene="dnaA"
                     /locus_tag="KLK57_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HBE0891976.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            23732..23812
                     /locus_tag="KLK57_001585"
     CDS             23732..23812
                     /locus_tag="KLK57_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0891977.1"
                     /translation="MTIDKNWLNRSNKDLGRSLRFTHQPV"
     gene            24042..24182
                     /gene="rpmH"
                     /locus_tag="KLK57_001586"
     CDS             24042..24182
                     /gene="rpmH"
                     /locus_tag="KLK57_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HBE0891978.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            24199..24558
                     /gene="rnpA"
                     /locus_tag="KLK57_001587"
     CDS             24199..24558
                     /gene="rnpA"
                     /locus_tag="KLK57_001587"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HBE0891979.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            24522..24779
                     /gene="yidD"
                     /locus_tag="KLK57_001588"
     CDS             24522..24779
                     /gene="yidD"
                     /locus_tag="KLK57_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HBE0891980.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            24782..26428
                     /gene="yidC"
                     /locus_tag="KLK57_001589"
     CDS             24782..26428
                     /gene="yidC"
                     /locus_tag="KLK57_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HBE0891981.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            26534..27898
                     /gene="mnmE"
                     /locus_tag="KLK57_001590"
     CDS             26534..27898
                     /gene="mnmE"
                     /locus_tag="KLK57_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HBE0891982.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGLKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVEVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT         6749 a         7582 c         7146 g         6564 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gtagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgactatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gtttaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      421 gtaggttact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      481 tgtggagtcc tggctatttt gccaatagct gcggaggtgc gccaatatcc gtcatccgcc
      541 aatacattga acaacagcaa acacctggtt aggtggaaaa ccgcgcctta tatccccgcc
      601 ctgaaggacg gggttttacg gcgcaccgga taaatttggt tatgcgccgt cagccgtgct
      661 aaagaatacg gaccagcacc caacggatgc cagtccacac aattaacgta actctggcca
      721 gtacgctttg ttggcctcca gcaactcatc cagaatagct ttggcgaccg aggcgctcgg
      781 taccgttttc gataacgtaa tcgcctgcca caggtgctga tatgaacgct gttcccaggc
      841 gtccacgacc agtttttcca ccgccacttg ctgactcatt aaccctttct gaaactgcgg
      901 gatatcgccg accaccagtg gttctggtcc gttgtgcccg accagacacg ggatctcgac
      961 catcgcttca tcatcaaagt tatggattgc cccgttgtta ggcacaatca gcaacatccg
     1021 ctcctgagtg ttgaaggcaa ttgccgccgc cagatcgacg atgtatgacg catgttcgtc
     1081 aatctccagc ttggccgccg ctgaatttcc tgccgctgta atggcgcggc aagcatcgaa
     1141 aacctgtttc tcgcgatgtt ccattcacct cattcgcgcg ggtatgttca gggttggaat
     1201 gctgaacaac ataatccggg aagagataat atttcagata ggtgttcggc aatgtatccg
     1261 ggtccagtgc ctggacatcc cgcgcttttg cataggtgtc gttccagctc gcttcagtat
     1321 gtgtatcttg ctgcggtgga acataaccgt gcttcgagac atattgccgc agctggggca
     1381 tcaggtcgtt gccctgcaaa tcttcaattg atgtccacca gccaaagtgg ttcaggccgt
     1441 agtagcgcac gcgcatctgt ttgcgatctt gcagcccaac aatttgcgcc atccggcttt
     1501 caataccgat tggcatgtca cagatgttga ggattttcgc attcgggcgc agacgacgcg
     1561 tggcttctgc gacaatggct gccgggttgg agtagttgag catccaggca tttggtgaat
     1621 atttttccat ataatccacc agttccagga cgccgccaat ggaacgcatg ccgtacgcta
     1681 ttccgccggg tccgcaagtt tcctgaccaa caacaccgtg gcgcagtggg attttttcat
     1741 ccagttcgcg catcgggtac ttgccgacgc ggatgtgcgc catgacaaaa tcaacgtcgc
     1801 tgaacgccac ttcaggatcg gtggtgtaac taaacgcaat gtccggcgct ttctctttaa
     1861 ggatgacttt acaggcttcg gcaatcactt cctgccgcgc gccatcgtta tcataaaatt
     1921 tcagtgcacg aagcgggaaa cgctcctgat tcgccaggag catcaacacg atgcctggcg
     1981 taaaggtgct tccaccgcct gcgacaacca ctgagaattt ggtcattata ctgcctccgt
     2041 aatggcaaca ttttctgctg attgatgaga attaattaag ctatcgagct gttcacgcag
     2101 ctgggataca tgcagaccaa tgatcacctg aatggcatcg ccactacgga agacgccgtg
     2161 cgctcccagc tttttaaaga cttcgtcatc cagcgtttgt gacatgtcat gcagtgcaat
     2221 gcgtagacgc gtcgcacaat tgttaatgct ggagatattg ccgaccccgc ccagggcttg
     2281 caggataccg gcagcctgat ccagctcttt ttttggctct gccgcggtgg tttggcctcg
     2341 cgaggctttg tattcggctt ttgagtagag tttcacttcc gcatcttcac gtcccggcgt
     2401 gcacatgttg aactgcagaa tcagggtgcg gaaaaccacg aagtacagca gggtaaagca
     2461 caacccaatg gcgatttggg tcagcatcat atccgcgtgg ttgctgaaca tcgggatcca
     2521 gttttgcggt aaaacctggt caatcagacc tccgcccatg ttgcccacca caccaaagag
     2581 atacatcacg gtcgacattg aggccgcaag cacagcgtgt accgcaaaca gcaacggtga
     2641 aatgaacagg aaggtaaatt ccagcggttc ggtaattccc accagcatgg cggttaaggt
     2701 tgcgggaatc agcaagcccg ccacttttac ccgattttcc ggtgcggcag tgaagtacat
     2761 cgctaaagaa atgcccacgg caccaaagat ttttgagtta ccgtgcaggg caaaaccgcc
     2821 ttccgggaac aacgatttca gcggctcggc gctcaaactg aactcttgca gatgctgcgc
     2881 ccagtacatc tgaatgccgc cttcaacagc tgccggaccc aagataaacg gtccgtagat
     2941 gaagtggtgt aaaccggttg ggatcagaat acgttcgagg aaggtgtaaa cccagacccc
     3001 aagcgcaccc gccgaacgca ggaacgcttg cagagattca atccccgttt gtacttttgg
     3061 ccagccgagc aatgtcagcc aggcacaggg gatcatcacc aggaaggcga taatcaccac
     3121 ataagacgtc ccctggaaaa tgccgagaaa aaccggcagt tttttatcga acagacggtt
     3181 atgcagcgcc gtcacaatgc cggaaatgat aattgcgccg ataatgctgg tatcgagggt
     3241 tttaatcccg gccatcattg tcagaccgct acctgccacc gcgtcctgag tgaaatcgac
     3301 gccgaagtag cttccccagg tcattcccat cgcgttgatg aaatagttcc aggtcaggaa
     3361 actcaccatc accgccagac aagcacgtcc ctgcgcttgc ttagcaaggc caatgggtaa
     3421 accgacagca aaaatcagcg gcatattacg gaataccgtc caaccgccct cttcaataat
     3481 gtgtacgatt tgcgcgaata aactgttcgg atcggtcagt gattccccga caaacatcgg
     3541 gttttgcagc aagatggcaa gacccaccac aatcccggcg aagggaaaca gcagcactgg
     3601 cgtaaacatc gcgccgccaa agcgttgaat ttgactgagc atttccacat cctcttttct
     3661 caattctgta agtaggaagc ctgattggtt atgcggcagg cctggagcaa ggataggaag
     3721 atgcgatcgg cagaacatga cgacccaatg ggattcgtga tcgctttcat gcttttcgtt
     3781 gggcggtaga gatctacgtt ttcgcgaaaa agtagacatc tgtgagcaca ggtacacagc
     3841 atcgaacaca tctttaaaaa aaagatgttt tttcaatcga ttaagcagaa cttgtgtgcg
     3901 cattacccgg gcttgcaggc aaaaaagaga tctagagatg atctacaaaa gcattgcgga
     3961 gcggttaaga attcgactta actccgcaga tttcacgcta aacagccttc ttccgggtga
     4021 aaaaaagctg gcggaagagt ttgcggtatc gcggatgacc atccgtaaag ccattgacct
     4081 gctggtagcg tgggggctgg tggtccgccg ccacggtagt ggcacttacc tggtgcgcaa
     4141 agatgtactg catcaaaccg ccagtctgac cgggctggtg gaggtgttaa aacggcaggg
     4201 aaaaactgtc accagccagg tgctgatttt tgaaatcatg cctgcgcctc cggccattgc
     4261 cagccagtta cggattcaaa tcaacgagca gatctacttc tcccgtcgcg ttcgttttgt
     4321 ggaagggaaa ccgctgatgc tggaagacag ctatatgccg gtaaaactgt tccgtaatct
     4381 ttcgctgcaa catctggaag ggtcgaagtt tgaatatatt gaacaagagt gcgggatttt
     4441 gattggcggt aattatgaaa gcctgacgcc ggtgctcgcc gatagactgc tggcgcggca
     4501 aatgaaggta gcggaacaca cgccactgct gcggatcacc tcgttgtcat atagcgagag
     4561 cggggagttt ttgaattatt cagtgatgtt cagaaatgcc agcgaatacc aggtggagta
     4621 ccatttacgg cgactccacc cggaaaagag ttaaccgata ctccagaaga gcaccgccag
     4681 taattggggg gtgataatgc gcaggaacat cactaacgga tagacagtgg cgtaagagag
     4741 cgccgccgca ccgctagttg gatgaagatt attagcaaac gccagcgccg gaggatcggt
     4801 catggaacct gccagcatcc cgcacatggt caggtaattc attttggcta acatccgcgc
     4861 cagaatgcca acagtaatca gcggaacggc ggtgatcagg gcaccataac caatccagct
     4921 tagcccttcg ccattgacca gggtattcac aaaatcccca ccagatttca gaccaacgac
     4981 cgagaggaac agcacgatcc ccagctcccg cagcgcgagg ttggcacttg gcggcataaa
     5041 ccagtacagc ttgccgatac tgccgatacg cccgaggatc aacgccataa tcagcagacc
     5101 gcccgccagc cccagtttca acgcggccgg gaatcccggc acaaagacgg gaatagaacc
     5161 taacaatacg ccaagcccga tgccaataaa cactggcagc atctgaacct gttgcagttt
     5221 ttgctgcgca ttccccagca cattggcaac ggcatcaatt gcggacggac gccccaccag
     5281 gttgaggata tcgccgaact gcaggctgat atcgccactg gcgaccagtt cgaccccggc
     5341 acggttcagg cgcgagataa caacgtcata gcgttcttta aagtgcaggt cgcgaatacg
     5401 ttttccgagc acgttttcat tggtgaccac cacacgctcg acgcgcaaat cagtgtcttt
     5461 cgtggacagc gaagtatcga cctcctgacc aatcaccagt tgcgcattat gtaaatccgc
     5521 tggctgaccc accagatgca gcaaatcgcc cagttggata atggtatctg gcgaaggaac
     5581 ttttagggtt tcttcgcgtt tcagacgcga gcagataatt ttgtcgccgt tgagaatcgg
     5641 tacatcttta atggctaaat catgcaggtt agggttctca acgcgaatat tgatagtctt
     5701 gatcagcgcg ccgccattgg tgcgtgaaga ctcgtgctgc tgagcttctg tctcgacatt
     5761 gacgcggaaa ataacccgca acatccacat ggtgaacaaa atcccgcaaa tgccgaatgg
     5821 atacgccatc gcgtaactca tccccatctg atcgaccatt tccattggtg tacccaggtc
     5881 gcgcaaaatc tgctgccctg cccccagcgc tggcgtattg gtaaccgcac cggagaaaat
     5941 ccccagcact accggcagtg gaatatcaaa cagtttatgc aggatggcgg taaccagacc
     6001 accgatgatg acgatcagaa cagcaaacag gttgaggcgt aatccggaga cgcgcaatga
     6061 ggcaaagaag cccggcccta cctgaatccc gatggtataa acgaacagga tcaggccaaa
     6121 ttcctgaata acatgcagca tatcgctact taatgtcatc cccgcctgag aaacaaaatg
     6181 gccgacgatg atcccaccaa acagcacgcc gccaatacct aatcctatgc cgcgaaattt
     6241 gacgttgccg ataaacaaac cgacgactgc caccaaagcc agaatactga ccgttaatgc
     6301 tatatcactc atcgtccatt tctcttgaga taacatttta attgctacgg attatggctg
     6361 aatcggtgaa gcagatttgc cttatagcgc acattattac gggaaagaac gtgccgaaat
     6421 atcttaaaca gtcgaaattt aacgcattgt tgaagaagaa agacaaaaaa agccccacca
     6481 gaatggcggg gcaaagagaa tagctagtta gctatttaac gcgggacgtt cgctgatagc
     6541 gatacgctgc gctgcgatgg gttcaggctc attacgaatt aaatcaatat gcagtaaacc
     6601 gtttacgaaa gttgcgccag agacttccat attttcagcc agcgtaaagc tcaggctaaa
     6661 tggctgattc ataagccctt gatgcagcca ttttttctct tcttttggct gctccggcgt
     6721 gccttttacg ctcaggcgcg taccttccag ttgaatctct aaatcttcct gacggaaacc
     6781 tgccagcgca agggtaatgc ggtagtggtt atcgtcgctt ttctcaatgt tgtacggcgg
     6841 gaagctctga ctttcaccgg cgttttgcag tgcgttggcc agtttgtcaa aaccgatcca
     6901 ttgacgcatc agtggggata aatcgaagtt acgcatagtc atttctcctt ctaagaagcg
     6961 agtaagtacc tgcaaatccg aagattcgca tatgctccct gacggcgagc atggagatgt
     7021 caggccgcgc caggcggcct taaggaatta gttgatttcg atacggcgcg gttttttcgc
     7081 ttccggaatc acgcgttcga gatcgatata cagcaaacca tttaccaggt tagcgccacg
     7141 aacatgaatg ttctcagcta actggaattt gcgttcaaag ttgcgttcag cgatgccctg
     7201 gtacagatag gtgcgctctt tttgttcgtc ggcgtgagca cctttcacca ccagcagatt
     7261 atcctgggcg gtaatttcca gttcgctctc agcaaaacca gccacagcga tagcaatgcg
     7321 gtaatggttt tcgtctacca gttcaacgtt atacggaggg tagccgccat tactctggct
     7381 ctggttgttt tctaagtggt taaacaaacg gtcaaatcca atagcagaac ggtaaagcgg
     7441 ggataaatca aagttacgca taatcaatag ctcctgaaat cagcgagaat gtaagacctt
     7501 ccacaatgga caggtcaggt agccagaaca cccatcaggc gcgtcctcat cgactacgat
     7561 gtaaaaatgg gtctggaaat gaacttttca agccttatca gcggactttt ttttgcagtt
     7621 tatggtctat tgcatagact gagggggcag cacgaaacgt taaagtgtga tagccgccac
     7681 agagcgacgt aatgatgatg ttattttcag caagggatcg ctataactca tcaggccaac
     7741 acccacgatc aacagatgaa tttattatga taagaaatgt gttgttagcg ttcatgatat
     7801 gcagcggaat ggcattactc ggaggatgct ccagcgtgat gtcccatacc ggagggaaag
     7861 aaggaacgta tcccggcacc cgcgccagcg ccaccatgat tggcgatgat gaaaccaact
     7921 ggggcactaa gtcgctggcg attctcgata tgccgtttac cgctgtgatg gacacgcttt
     7981 tattaccgtg ggatgtgttc cgcaaagaca gctcggtgcg ctcacgcgtt gaaaaaagcg
     8041 aagccaacgc gcaagccacc aatgcggtta ttccaccggc cagaatgcct gataactgat
     8101 ttagcgatca gtttcggtga tccacagctg gccaccttcc atccacgcca gccattgacc
     8161 atccggcgag aagaccacgg cgtccgcaga aggtggattg gcgtggtttt cggttaaata
     8221 ctcaacctcg ccgctttgcg catgggcaca agcaattcga ttatccagca caaagcccaa
     8281 ccattctcct gacggatgcc agttaaatgc agactgaata tccgttttgt tatgggttaa
     8341 ctggcgcggc tcgccgccct gtggcgagat aagccacagt tgcacaatgc cgttatcatc
     8401 acgcattaaa aacgcgattt gcgtaccctg cggattacag cgcacccagt ggcgcgggac
     8461 gttgactaac cccggataag cccgatggtg ggtaaaggtt aaacgtcgct gcacgacgcc
     8521 acgcggtggc gcgggcaggg tggtttccgt tccacttaac ggcgcatcac ctgccgcttt
     8581 ccagccagct tcatcttgcg gcaactcaac gataaacagc tccggcactt tctcaccctt
     8641 tggcgaaagt gtgtcgccaa taaacgccag cgcgtgattt cctacccatc cttcttcata
     8701 agcacgattg atttcatcac tgccaggctg cggcgtgggc gtggttttac tcaccagcac
     8761 acaccagtgg ctgccgctgt attcacgcgg atgctgcttt tgtacgttga ccggaccaaa
     8821 cggcgcagca acaccgacgt ttcgcaaatc cagcgccgga tcgagttcat gcattacatg
     8881 gtcgttatag gtaaagctca ccctttcacc gttcgggcta aagacatgca catggctgcc
     8941 gccgcgcagc gctcctggcg tatacggcgc ggtaatatcc attgcatcga gattgctcac
     9001 cttgccgcct tcagcaatga ctccgcgccg atgatggaag tcgtaatgcc atgtttcatc
     9061 aggattttcc gggccgtgaa taaagacata tttctctgac ttcggatgaa cggtcaccac
     9121 gccgacgtgt gcgccctgtg acgcacagta gataacctcg acctcaccgg tatggatatt
     9181 cacacgctca atggtctcgc cggtaaacga cgccccagaa ggacgcacgt caaataccag
     9241 ccactggctg tcgggcgtcc aggtattggt attggtgagc aggtgattac ggggagcaaa
     9301 ggttatctgt ttcatggcgc ggtcctgata caaaacgggt ccagccatca tacttcacaa
     9361 ggacttcact ttcagacaat agtcttttgc catcgtgatg aatgtgagtg aaaagatgga
     9421 acatttcgac gtggcgatta ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt
     9481 agccggcaaa atgcaggtga tcgcgctgga taaaaagcac cagcatggta ctgaaggttt
     9541 cagcaaacct tgtggcggtc tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg
     9601 gctgacgctt cctgtcgatg tgatcgctaa tccgcagatt ttcagcgtca aaaccgtcga
     9661 cgtcgccgca tcgctcacgc gtaactacca gcgaagctat atcaatatta atcgccatgc
     9721 tttcgacttg tggatgaaat cgctgatccc cgccagcgtt gaggtttatc acgacagcct
     9781 gtgtcgaaaa atctggcgtg aggatgataa atggcatgtc atttttcgtg cagacggctg
     9841 ggagcagcat attaccgccc gctatctggt cggtgccgat ggtgccaact cgatggtgcg
     9901 gcgacatctc tacccggatc atcaaatccg taaatatgtc gcaatccagc agtggttcgc
     9961 agagaaacat ccggtgccgt tctactcctg catctttgat aatgcgataa ctgactgtta
    10021 ttcatggagt atcagcaaag acggttattt tatctttggc ggtgcctatc caatgaaaga
    10081 cggtcagacg cgtttcacga ctctgaaaga gaaaatgagc gcctttcagt tccagtttgg
    10141 caaagcggtg aaaagcgaaa aatgcacggt gctgtttccc tcacgctggc aggattttgt
    10201 ctgcggtaag gacaacgcct ttctgattgg tgaagcggcg ggatttatca gcgccagctc
    10261 gctggagggg attagctatg cgctggatag cgcagacatt ctgcgttcgg tgttactgaa
    10321 gcagccagag aagctcaata ccgcttactg gcgcgccacc cgcaaactgc gtttaaaact
    10381 cttcggcaag atagtaaaaa gccgatgcct gaccgcaccg gctttaagaa agtggattat
    10441 gcgcagtggt gtggcacata ttccacagtt gaaggattag ccaacgcgct tcacatcgcc
    10501 caccagcagg atataagaga gcgcgccaat caacgcgacg gcggagatat aaaccagtgc
    10561 aggtgcgaaa ccgtaaccct gcgccaggta ccccaccacc agcggaacgg tgatgccgcc
    10621 cagaccaccg gcgaagttaa acacgccgcc ggttaaacca atcaggcgca tcggtgccag
    10681 agaagaaacc agcgaccagg taattgaagc aaaaccgtta ccgaagaatg ccagcgccat
    10741 caggcacata atcatcatcg gatcgttagt gtagttagcg cccataatgc aggtggagat
    10801 cagcaagccg cagataatcg gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac
    10861 cagcagatcc gctacccagc cagagagcag gacgccgaca aacgccgcga ggaatggcac
    10921 cgtggtcata aagcctgctt tcagcgccgt gattcctttt tcctgggtta aatagttcgg
    10981 gaaccaggtt aagaaaaacc acagtgtaga agccaccgca aattgcccaa gatagacgcc
    11041 gatcagttta cgatggaaca ccagtttcca gtctttggct gttaacggct gacgtgcctc
    11101 tttcttcact ggcgcatcac catccaccag accgccgcca tcacgaatgt aatccagttc
    11161 agctttgctg atgcctttgg tcaggcgcgg cggctgataa accttaaacc aaatcagcga
    11221 ccagataatg ccgataccac cagtcacaat gaacacccag tgccagctca acatctcctg
    11281 aatccagatc agcagcggag tcaaaaacgc cagaccgaca aactgaccag acgtataaaa
    11341 accaacggcg gaggcgcgtt catgttccgg gaaccagctg gtcaccatcc ggttattggt
    11401 cgggaaagcg ggcgcttcga aaataccggt tatcgcacgc agaccaatta atgacattaa
    11461 tcccgtggca aagccctgga ataaagtcgc cactgaccag ccaaatatcg caataaaata
    11521 agtcacgcga gaacctacgc gatctaaaaa ccaaccgccg gggatctggc atagcgtata
    11581 aagccaggcg aaggccgaaa atacatagcc catttccgct ttcgtaatgc cgaactcttc
    11641 ctgaatatgg gcggaagcca cggccaggtt agcgcggtca acataacaaa tgaccaccgt
    11701 aataaagatc atcaccagcg tcagataacg ccgacgcccc ggctttgctg cattaacggg
    11761 aatatccata gcgagctttc tccagatttt gggcatagcg aagccgctca ccatgccctg
    11821 taatttacag agggttatat ttttgtattg ctgttttagt gcccgatgag gggcttacgt
    11881 ggcaggaatt accactctgc tacgctgtta tcttcatgac gccagagtgg attacgccag
    11941 tccggggcat ttttactgaa ctcaataact ttagcttcgt cgatttccac gcctaagccc
    12001 ggtttcgtta gcggtttaaa gaagccgcca accatgctga agtcttcttt gtttttcaca
    12061 aagtcgagta actccgcgcc tttgttgtaa tgaattccca tactttgttc ctgaagtacc
    12121 gcgttatagg aaacaaagtc gatatgcaga caagccgcca gtgcaatcgg tccgagcgga
    12181 cagtgcggcg caagggtcac gtcataggct tctgccattc cggcaatttt gtagcattcg
    12241 gtaataccgc ctgcatggga gagatccggt tgtagaatcg aaataccgcc tgcctccagc
    12301 acgcgtttaa aatcgaaacg tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt
    12361 tgtgccgcca atttcgggta gtattcggct tgttccgcca gcaccggctc ttcaataaac
    12421 agcgggcgat acggctccag ttctttaatc agcactttcg ccattggcgc gctgacgcga
    12481 ccgtggaaat caagaccaaa ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg
    12541 gtgttgactg ccgcatctac cgcgcgggag ttatcaatta gccccagttc ttcacaaccg
    12601 ttcagtttga aggtatcgaa gccgatttcg cgcagggttt taataccgtc gatcacatcc
    12661 gccggacgat cgccgccgac ccaactgtag gctttaattt tgtcgcgaac caggccgccc
    12721 atcagttgcc agaccgacgc attcagcact ttacctttga tatcccataa cgcctggtcg
    12781 atcccggcaa tggcgctcat caggattgga ccgccacgat aaaatccggc gcgatacatc
    12841 acttgccata agtcattgat gcgctatgga tcctgaccaa tcaaatagtc acccagctcg
    12901 tgaactgccg cttccaccgt gcgggcgcgg ccttcaatca ctggctcgcc ccagccgacg
    12961 acgccttcat cggtttcaat tttcaggaac atccagcggg gaggtaaacg atacgtggta
    13021 attttggtga ttttcattgc actgcctctc gatacgcctt aacaaatgct gctgcctgct
    13081 gcgcggtgcg ttctacggac tgcccggcgc gatagagatc gctgcctaag cccgccccag
    13141 cacaacctgc gtctatccac tgcgccaggt tttctggcgt cacgccgcca acggcaaaga
    13201 ctgcgatgtc cgatggcaat accgctttta acgctttgat gtattgcgga ccaaaagccg
    13261 atgacggaaa tattttcagc gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg
    13321 ccgtcgcgca gccggggcag acggtcatgc cgtagcccac cgcacggcgg atcacttcac
    13381 tatggatatt gggcgtaacg atgagctgac agcccatcct ggcgagcgca tcgacctgtt
    13441 caggtttcag taccgtacct gcgccaatca acgccttgtc gccatacgca tcaacgatgg
    13501 cgggaatgct ttgctcccat tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg
    13561 cgtcaatcac cgcgccaaca tgcgccagcg cctcgtcggg cttaatgccg cgcaaaatgg
    13621 cgatcagcgg aagtttagtt tgccactgca tgagcgatgc tccttatacc agcctgaaat
    13681 gccgtgtcgc ccgccaccgc cgtcacgtcg caacccatcg cctgaaaggc ttgctggtag
    13741 cgcgcggtca acgatgttcc ggcgacaagg gtgatggcgt gttgatgggc cacatagtcg
    13801 cgcatactgg cgacctctgc gccaatcaac aaaccagaga gaaattcact gacctgttca
    13861 cggggaagtg ttcccagcac atgcgaggca cgaacttcaa aaagctgcgg caatatggcg
    13921 ggcgtattaa gaccacgctc aaggccagcc gtgaaggcat cggcagagtt ttcctgcggc
    13981 ggcaaacctg caccaatcaa tgagtgattt aacagtaaat gatgtaattc accggtcatc
    14041 acggtgcgaa aatcgttgat ttgctggcta tcggcctgca cccatttgca atgcgtaccg
    14101 ggcatgacat aaagagagga aggagccaga gctcgcgcgc cgatcaattg agtttcttcg
    14161 ccgcgcatca cattgtggtt atcatcatga gagacgcata atccgggaat aatccagata
    14221 ttgtcgccaa cagacgttaa ttgttcgcca atagacgaaa aatgggcagg aacagataaa
    14281 tacggagcaa ctttccagcc gacattgctg ccgaccattc ctgccattac cactggcgta
    14341 ttctcttcac gccagtcggt cgtgacttct gctaacaccg cagccggaga ttttccgttc
    14401 aggcgcgtga cgcctgcttc tgattgcctg ctctccaggc agtggtcgcc ctgataaagc
    14461 caggcgcgca gattggtcga tccccagtca attgcgatgt agcgagctgt catgtgattt
    14521 cctttaacct tcgtgtcgag ctggcgatca tggtaagcgc cgcctgctct gccgcatcgc
    14581 cgtcctgatg ccgtatcgca tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt
    14641 tggcctcatc gcccatccag gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa
    14701 tgctaagttg ctgtaacacc gggttatgca ccgactgcag caccgcctcg tggtagcgaa
    14761 tatccgcttc gttaaacgct tcgcggtcct gattgttggc aatcatctcg ttcagcgccg
    14821 attcaatctg cgccagatcg ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg
    14881 gttccaccag atttcgcact tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt
    14941 ccagcaccca ttgcagtacg tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa
    15001 acgcgccgcg ataacgtttc atttcaatca gccgtttcgc catcaacgaa cggaacacct
    15061 cacggatgat gttgcgcgag gttgcaaact cctcacagag ttccgcctca gccggaagcg
    15121 gcgagcctgg tacgtatttg ccgtgaacga tctgtttacc cagcgtaatg acgatgcgat
    15181 cggttttatt gagagtcatg gagagtcctt gtgctctgta tgttcttctc tactttaccc
    15241 cgatcgatgc ataatcagga aagtatcaat cgcagtcaga caccatcacg gggctatcgc
    15301 aggcaaagga tagtaatgga acaaaaggct atgtttttgt aggggaatcg ctggattacc
    15361 ttatcacaga tggtgccgat gacatcgtta agatgctcaa ggatccagct cttaaccggc
    15421 acaatattca ggttgctgat gacgcaagat ttgttttaaa tgcggggaaa aagaaattta
    15481 ccggcacaat atcactctat tacaactgga gcacagaaga agaaaaagca cgagtgacac
    15541 aatatcgttt tgcttgtggt gttcagcact gtacccggac agtagaaaac ctggaaggta
    15601 ctatccatga gaaaaataaa aacatggatt actcaaacgt aatggctttc taccatccat
    15661 ttaaagtgcg attttatgaa taccattcac ccagaggcat tccggatggt gtttccgcag
    15721 cattactgcc agtgactgtt acgctggaca tcattactgc accgctgcaa tttctggttg
    15781 tatatgcagt aaaccaataa tcagtaagcg ggcaaacgcg tttatgctgt ttgcccgccc
    15841 acagattaat tcagcacata cttctcaata gcaaacgcca cgccatcttc aaggttagat
    15901 ttggtgacaa agttcgccac ttctttcacc gaaggaatag cgttatccat cgccacaccg
    15961 acgcctgcat attcaatcat tgcgatgtcg ttttcctgat cgccaatcgc catgatttct
    16021 tccggtttaa tacctaacac gtcggccagt gatttcacac ccgtaccttt gttaacgcgt
    16081 ttatcgagga tttcgaggaa gtacggcgca cttttcagca cggtatattt ctctttcact
    16141 tcctgcggaa tacgcgcgat agcctggtcg aggatggcgg gttcatcaat catcatcact
    16201 ttcaggaact gggtattggg gtccattttc tccgcttcgc agaacaccag cggaatagtg
    16261 gcaacgaaag attcatgcac tgtgtagtag ctgatatcgc ggttggcggt gtacagcgtg
    16321 gtgcggtcca gggcgtggaa atgagaaccg acttcgcgag agagtttttc caggaaacga
    16381 tagtcgtcat agctgagagc agtttgcgcc acggtgctac catcagctgc cttctgtact
    16441 agcgcgccgt tataagtaat gcagtagtcg cccggctgtt ccatatgcag ctctttcagg
    16501 tagttgtgca cacctgcata cgggcgaccc gtcgttagca cgacattcac gccacgggcg
    16561 cgagctgcgg caatcgcatt tttaacggcg ggtgaaatgg tgtgatcggg cagcagaagg
    16621 gtgccatcca tatcgatagc aatgagtttg atagccatga gttccccagg tagattggtt
    16681 cctgacccat gctaacgcga ttccgctcaa aaatcagtac aacacccgag ggaaaagggg
    16741 gatgcaacgc gggttgcatg ctccccattt gcttagcgga agagtttccc tttcagcagt
    16801 tccatacctg cggaaagcag atcgttattg gcttgtggtg acacttcacc ttgcggtgag
    16861 agcgcatcaa taatcttcgg caattgctct gccagtaaac tggaagctgt actggtatcc
    16921 acgccaagtt tttgcccgag atcggacacc gcatttgtgc cgagcgccga ttccagttgc
    16981 tcgccactaa ccgattgatt gccctgttga ttactcagcc aggttgagag aatggcccct
    17041 aagccgccac tttgcagttt ttccagcagc acctgaatgc cgccctgctc ctcaacccaa
    17101 cttaaaatag cctgatattt ccccgcatcg cctttcagaa aggcaccgac aacttcatca
    17161 aaaagcccca tgataatcac ctgtaaagcg ttacgtgttg acccaaaaag tatagatttg
    17221 cgggatgata attgcggatt gcagaaataa aaagggcgga gatgatctcc gcccttttct
    17281 tatagcttct tgccggatgc ggcgtgaacg ccttatccgg cctacaaaat catgaaaatt
    17341 caatacattg caagattttc gtaggcctga taagcgtagc gcatcaggca cgctcgcatg
    17401 gttagcgcca ttaaatatcg atattcgccg ctttcagggc gttctcttca ataaacgcac
    17461 ggcgcggttc aacggcgtcg cccatcagcg tggtgaacaa ctggtcggca gcaatcgcat
    17521 ctttaacggt aacgcgcagc atacgacggc tttccgggtc catggtggtt tcccacagct
    17581 gttccgggtt catctcgccc agacctttat aacgctggat ggagaggccg cgacgggact
    17641 ctttcaccag ccagtccagc gcctgctcga agctggctac cggctgacga cgctcgccac
    17701 gttcgataaa cgcatcttct tccagcaagc cacgcagttt ctcacccagc gtgcagatac
    17761 gacgatattc gccaccggtg ataaactcgt gatccagcgg atagtcagta tccacaccgt
    17821 gggtacgcac gcgaacaatc ggttcaaaca ggttttgttc ggcattggtg tggacatcaa
    17881 acttccactg gctgccgtgc tgttctttgt cgttcagttc gctgaccagc gcgttcaccc
    17941 agcgggtaac ggtctgctca tcagagaggt cggcttccgt cagcgtcggc tgatagataa
    18001 gctctttcag cattgctttc ggataacgac gctccatgcg gttgatcatt ttctgcgtcg
    18061 cgttgtactc agacaccaat ttctctaacg cttcgccagc caatgccggt gcgctggcgt
    18121 tggtgtgcag cgttgcgcca tccagcgcga tagagatctg gtactgatcc atcgcttcgt
    18181 cgtctttaat gtactgttct tgcttgcctt tcttcacttt gtacagcggc ggctgagcga
    18241 tgtagacgtg accgcgttca acgatttccg gcatctgacg atagaaaaag gtcaacagca
    18301 gcgtacgaat gtgcgagccg tcgacgtccg catcggtcat gatgatgatg ctgtgataac
    18361 gcagtttgtc cgggttgtac tcgtcacgac cgataccaca gccaagcgcg gtgataagcg
    18421 tcgccacttc ctgagaagag agcatcttat cgaagcgcgc tttctcaacg ttgaggattt
    18481 tacccttcag cggcagaatc gcctggttct tgcggttacg cccctgcttc gcagagccgc
    18541 ccgcggagtc cccttccacc aggtacagtt cggaaagcgc cggatcgcgt tcctggcagt
    18601 ctgccagttt gcccggcagg cccgctaagt cgagcgcacc tttacggcgg gtcatttcac
    18661 gcgcgcgacg cgccgcttca cgggcacggg cagcatcgat aattttgcca accacgattt
    18721 tcgcgtcggt tgggttttcc agcaggtatt ctgccagcag ttcgttcatc tgctgttcaa
    18781 ccgctgattt cacctcagaa gaaaccagtt tgtctttggt ctgggaggag aatttcgggt
    18841 ccggcacttt cacggaaacg accgcaatca ggccttcacg cgcatcgtca ccggtggcgc
    18901 taactttggc ttttttgctg tagccttctt tgtccatgta ggcgttcagg gtacgggtca
    18961 tcgccgcacg gaagcctgcc aggtgagtac cgccgtcacg ctgcggaatg ttgttggtaa
    19021 agcagtagat gttttcctgg aagccatcgt tccactgcaa cgccacttcg acgccaatac
    19081 cgtctttttc agtggagaag tagaagatat tcgggtggat cggcgttttg ttcttgttca
    19141 gatattcaac gaacgccttg atgccgcctt catagtggaa gtggtcttct ttgccgtcgc
    19201 gcttgtcgcg cagacgaatg gaaacgccgg agttgaggaa cgacaactca cgcagacgtt
    19261 tcgccagaat ttcatattcg aactcggtca cattggtgaa ggtttcgagg ctgggccaga
    19321 aacgcaccat ggtgccggtt ttttcagtct cgccggtaac cgccaacggt gcctgcggta
    19381 caccgtgttc gtagatctga cggtgaattt taccctcgcg ctggataacc agctccagtt
    19441 tttgcgacag ggcgtttact accgaaacac caacgccgtg cagaccgccg gacactttat
    19501 aggagttatc gtcaaattta ccgcctgcgt gcagaacggt catgatcact tccgccgccg
    19561 atacgccctc ttccgggtga ataccggtcg gaatgccgcg cccgtcatcc tgtacagaga
    19621 cagagttatc ggcgtgaatg gtgacgataa tttctttaca gtgacccgcg agcgcttcgt
    19681 cgatagcgtt atctaccacc tcgaatacca tgtggtgcag accggtgccg tcatccgtgt
    19741 cgccgatata catacccggg cgcttacgca ccgcatccag ccctttcagg actttgatac
    19801 tggaggagtc ataagaattc gacatcaacg tttctcgctc atttatactt gggttaatcc
    19861 gttattttac ccttttccac ggtaaacatc ttcgaatttt cgtccgacat gtctataacg
    19921 tgttcagcac tgatcgcgct gacaaagacc tgtgattgcg tcgcttttaa gcggctggca
    19981 agcagcccgc gacgctcatc atcaagctca gaggcaaaat catctatcag gtagagacac
    20041 cgccgcccgc tttcacgggt gaggaactct ccttgcgcca gacgtaaggc gcacatcaac
    20101 agcttaagct gcccacgcga taaggtatct tccaccggcg caccgtcggc gcgaatgcgt
    20161 aagtccgctt tatgcgggcc atgcgcggtg taggttagct ggcgatcgcg ttcaaaatta
    20221 cgttccagca cctcagcata ttctgtctct ttctcccagc cgcgctggaa agagaaagtc
    20281 agagaaaact cagggagaaa ttgcttacaa gtatcggcca tatcagccgc gataccggcg
    20341 ctatactccg cgcgccaggt gctgatttgc tccgccagcg ggatcagctc tttatcccac
    20401 gggcgtagct gttcgtaacg tgtcacctgg cgcagcgccg cattgcgctg cttgagcaat
    20461 cgcttgagat tgctccaggc ggtgaaaaat ccgggttcgt tgtgaaagca tccccagtcg
    20521 aggaatgctc ttctgtattt ggggccgccg ttgagtaaag taaacccttc tggcgttatc
    20581 aactgcattg gcatcaggtg cgccagttcc gcgaccttat gcccgtctgt accgtcgatg
    20641 cggactttgc tgtcgccctg tttgtctttg gttaagccaa tcgctgtctc gcgctcttcg
    20701 ccctgtaatc gcccgtggag aacaaacgcc tcctgctcat ggcgaatgac gcgaccaatc
    20761 tgcaaactgc gaaacgcccg accatggccg agcgtataga tggcttccag cacgctggtt
    20821 ttgccactgc cgttggcacc taccagaaag ttaaagccgg gagataaagc gagatccgcg
    20881 gtttcaatgt tgcggaaatc gcggatcaac aagcgggtga gggacattac agtctcattg
    20941 gcatgacaac ataggcagcc gactgtgatg cggcatcttc aatctgcacg ctggaaaccg
    21001 aatcggtcag catcatgcgg acgttttcac atttcagcgc gttcagaaca tccagcacgt
    21061 agctgacgtt gaagccgatt tccatctccg caccgctata ggtaacgtcg aggatctctt
    21121 ccgcttcttc ctgttccggg ttgttggcgg tgattttcag ctggttttcg ctgacataaa
    21181 gacgtacgcc gcggaatttc tcgttagaga gaatcgccgc gcgagcaaac gcctgcttga
    21241 gcagatcgca gccagcttcc agatgtttgt ccgggttctt cggcagaacg cggcgataat
    21301 ccgggaagcg accatccacc agtttggagg tgaagataaa gtcgccaacg tgggcgcgaa
    21361 tgttgttgct gccaatctgt acgcgcagcg gattgtcgcc gccgtcgagc atacgcatca
    21421 gttcaatcac gcctttacgc ggcacgatca ccgaatggct tggcaaagat tgaccaattg
    21481 gcattgaaca gactgccaga cggtggccgt cggtcgccac ggtgcgcagt tcttcgcctt
    21541 cggtttcaaa cagcataccg tttaagtaat agcgaacgtc ctgatgcgcc atagaaaact
    21601 gggtcgcttc aatcagacgc ttcatcgtcg cctgcggcag ggtaaattcg acttcgctct
    21661 gccagtcatc gaggttcggg aaatccgccg ctggcagggt agacagcgaa aaacggctac
    21721 gcccggagcg caccagcatc cgttcacctt ccagctgtac ggcaatttcc gcgccttcag
    21781 gcagaccacg gcagatatca aagaatttgc gcgccggaac ggtcgtcgct cctggctcgt
    21841 gtggctgaac cagcgcaacg cgtgccacca tttccatctc gagatcagta ccggtcagcg
    21901 acaacgtacc gtcagcaacc tgtaacagca gattaccgag aatcggtagc gtaggacgac
    21961 cacctaacgg cccgctcacc tgttgtagcg gttttaataa atgctcacgt tctacggtaa
    22021 atttcatagg tttacgatga caatgttctg attaaatttg aaaaatcttc tttgatatcg
    22081 tggctctctt cacgcaactg ctcgatctta cggcaggcat gaagcaccgt cgtgtggtca
    22141 cgaccaccaa acgcatcgcc aatctccggc agactgtggt tagtcagctc tttcgccagc
    22201 gccatcgcca tctggcgcgg acgcgccacc gagcgggatc gacgcttgga aaggagatcc
    22261 gcgactttga tcttgtagta ctccgccacc gtcttctgaa tattgtcgat ggtgaccagt
    22321 ttttcctgca atgccagcaa gtcgcgcagt gcctcacgca cgaagtcgat ggtgatcgcc
    22381 cgtccggtaa agttggcgtt agcaataacg cggttcagcg ccccttccag ctcacgtacg
    22441 ttagatcgta gacgcttggc gataaagaac gccacttcgc ctggcaaacg aatgtcattt
    22501 tcgtcggcct ttttcatcag gatcgccacg cgggtttcca gctctggcgg ttcgatcgcc
    22561 acagtcagtc cccaaccgaa gcgggatttc aaacgatcct caacgccgtt gatctctttc
    22621 ggatagcgat ccgaggtgag aatgatctgt tgattacctt ccagcagggc gttgaaggtg
    22681 tggaaaaact cttcctgaga tcgttcttta ttagcaaaaa actgaatatc gtcgatcagc
    22741 agtgcatcta cggaacggta gtagcgttta aactcttcga tcgcgttgtt ttgcagggct
    22801 ttaaccatgt cctgaacaaa gcgctcggag tgcatataaa ccactttggc attcggcttg
    22861 cgcgccataa tgccgttacc caccgcatgc agcaggtgag ttttacccag acccgtgccg
    22921 ccataaagga acaatgggtt ataggcaccg ccagggttat ccgccacctg gcgagccgcc
    22981 gcgcgcgcca gttggttaga tttaccttca acgaagttat caaacgtgtg tttgacgttt
    23041 acgttagaac gataggtcgg ttctgccggg gccgggacgt tatcccaacc tgagcgcgta
    23101 gaaggcgcag cacgttgcgg ctgcgtttgc gccacctgtg caggggccgc gacgttgctc
    23161 gtcactgccg cttgtggcgt ttgcgtcacc ggtttggtgc cgacttcaaa acgcagctgt
    23221 ggggcatccg ctccgcagaa actggttagc agtccattga tattattaag gtacttgtcc
    23281 cgtacccaat cgaggacaaa acggtttggc gcgtacaggg ccagcgtgtt atcgctcagt
    23341 tccgcctgca atgggcgtat ccacatactg aattctgtgg ctggtaactc atcctgcaat
    23401 cgggcaagac actgctgcca aagcgaaagt gacacggcgg actccactcg aacaaaagtc
    23461 gataatgact aaggctgaaa cattcatgat tgttgacgta cgtcgaaaag accctgcttg
    23521 agggtgacgc acgaaccgct gtctgcggtt atatgcccga tcaagatcct gcaaaacgat
    23581 cgggaccgcg gatcatagcc taaactgcgc aagagatctt ctgtttctca cagattttcc
    23641 cgatttatcc acaggacttt ccagacctcg ctaagtgtaa acgatcctgc cgcgaggcgg
    23701 gcacgattta cgccgcatat tggaaaattt aatgaccata gacaaaaatt ggcttaatcg
    23761 atctaataaa gatctaggac gatccttgcg ctttacccat cagcccgtat aatcctccac
    23821 ccggcgcgcc atgctggttt tcactggtgt gaggtcgtac attttccctg cgaaaaggtg
    23881 cggaaaagcg cggtaaataa ggaaagagaa ttgactccgg agtgtacaat tattacaatc
    23941 cggcctcttt aatcacccat ggcttcggtg tccatcgttt catttttcgg cggatatcca
    24001 ataaagccat tgaatttatt caagtttagg tagaaatcgc catgaaacgc acttttcaac
    24061 cgtctgtact gaagcgcaac cgttctcacg gcttccgtgc tcgtatggct actaaaaatg
    24121 gtcgtcaggt tctggcacgt cgtcgtgcta aaggccgcgc tcgtctgacc gtttctaagt
    24181 aataaagcta acccctgagt ggttaagctc gcatttccca gggagttacg cttgttaact
    24241 cccagtcaat tcacattcgt cttccagcag ccacaacggg ctggcacgcc gcaaattacc
    24301 attctcggcc gcctgaattc gctggggcat ccccgtatcg gtcttacagt cgccaagaaa
    24361 aacgttcgac gcgcccatga acgcaatcgg attaaacgtc tgacgcgtga aagcttccgt
    24421 ctgcgccaac atgaactccc ggctatggat ttcgtggtgg tggcgaaaaa aggggttgcc
    24481 gacctcgata accgtgctct ctcggaagcg ttggaaaaat tatggcgccg ccactgtcgc
    24541 ctggctcgcg ggtcctgata gccctcattc gggtctatca acgcctgatt agtccgctac
    24601 tcgggccgca ttgtcgtttc actccaacct gttcaagcta cggaattgag gcattgcgca
    24661 ggtttggagt gataaaaggc agttggttga cggtgaaacg cgtattaaaa tgccaccctt
    24721 tacaccctgg tggtgacgat cccgtcccgc ccggaccatt tgataccaga gaacactaac
    24781 gatggattcg caacgcaatc ttttagtcat cgctttgctg ttcgtgtctt tcatgatctg
    24841 gcaagcctgg gagcaggata aaaacccgca acctcaggcc caacagacca cgcagacaac
    24901 gaccaccgca gcgggtagcg ccgccgacca gggcgtaccg gccagtggcc aggggaaact
    24961 gatctcggtt aagaccgacg tgcttgatct gaccatcaac acccgtggtg gtgatgttga
    25021 gcaagctctg ctgcctgctt acccgaaaga gctgaactct acccagccgt tccagctgct
    25081 ggaaacttca ccgcagttta tttatcaggc acagagcggt ctgaccggtc gtgatggccc
    25141 ggataacccg gctaacggcc cgcgtccgct gtataacgtt gaaaaagacg cttatgtgct
    25201 ggctgaaggt caaaacgaac tgcaggtgcc gatgacgtat accgacgcgg caggcaacac
    25261 gtttaccaaa acgtttgtcc tgaaacgtgg tgattacgct gtcaacgtca actacaacgt
    25321 gcagaacgct ggcgagaaac cgctggaaat ctccaccttt ggtcagttga agcaatccat
    25381 cactctgcca ccgcatctcg ataccggaag cagcaacttc gcactgcaca ccttccgcgg
    25441 cgcggcgtac tccacgcctg acgagaagta cgagaaatac aagttcgata ccattgccga
    25501 taacgaaaac ctgaacatct cttcgaaagg tggttgggtg gcaatgctgc aacagtattt
    25561 cgcgacggcg tggatcccgc ataacgacgg taccaacaac ttctataccg ctaatctggg
    25621 taacggcatc gccgctatcg gctataaatc tcagccggta ctggttcagc ctggtcagac
    25681 tggcgcgatg aacagcaccc tgtgggttgg cccggaaatc caggacaaaa tggcagctgt
    25741 tgctccgcac ctggatctga ccgttgatta cggttggttg tggttcatct ctcagccgct
    25801 gttcaaactg ctgaaatgga tccatagctt tgtgggtaac tggggcttct ccattatcat
    25861 catcaccttt atcgttcgtg gcatcatgta cccgctgacc aaagcgcagt acacctccat
    25921 ggcgaagatg cgtatgctgc agccgaagat tcaggcaatg cgtgagcgtc tgggcgatga
    25981 caaacagcgt atcagccagg aaatgatggc gctgtacaaa gctgagaagg ttaacccgct
    26041 gggcggctgc ttcccgctgc tgatccagat gccaatcttc ctggcgttgt actacatgct
    26101 gatgggttcc gttgaactgc gtcaggcacc gtttgcactg tggatccacg acctgtcggc
    26161 acaggacccg tactacatcc tgccgatcct gatgggcgta acgatgttct tcattcagaa
    26221 gatgtcgccg accacagtga ccgacccgat gcagcagaag atcatgacct ttatgccggt
    26281 catcttcacc gtgttcttcc tgtggttccc gtcaggtctg gtgctgtact atatcgtcag
    26341 caacctggta accattattc agcagcagct gatttaccgt ggtctggaaa aacgtggcct
    26401 gcatagccgc gagaagaaaa aatcctgatt cggtgagttt tcgctaaaat aagggcggtc
    26461 agttgaccgc cttttttctt ttcgtagggt ggataagcac cgcgcatccg ccacacaaag
    26521 caacaggaac atcatgagcg ataatgacac tatcgtagcc caggccacgc ctccgggacg
    26581 tggcggcgtt ggcatcctgc gcatctccgg cctaaaagcc cgtgaagttg ccgaaaccgt
    26641 gctgggtaaa ctgcctaagc cgcgctacgc cgattatctt ccgtttaaag acgccgacgg
    26701 cagcgtgctc gatcagggga ttgcgctatg gttccctggc ccgaactcgt tcaccggcga
    26761 agatgtgctg gaactgcagg gtcatggcgg tccggtgatc ctcgacctgc tgttaaaacg
    26821 cattctgacc attcccggcc tgcggattgc tcgccctggt gagttttccg aacgcgcgtt
    26881 tcttaacgat aaactcgact tagcccaggc cgaggcgatt gccgatctta tcgacgccag
    26941 ttcggaacag gcggcccgtt cggcacttaa ctcgctgcaa ggcgcattct ccgcacgggt
    27001 caaccatctg gtggaagccc tcacccactt gcgcatttac gtcgaagcgg caattgattt
    27061 ccctgatgaa gagatcgatt tcctctccga cggcaaaatt gaagcccagc ttaatgacgt
    27121 gattgccgat ctcgatgcag tgcgtgctga agcacgtcag ggtagtttgt tgcgcgaagg
    27181 gatgaaggtg gtgattgccg gacgtcctaa cgccggtaaa tcgagcctgt taaacgcgct
    27241 ggcgggccgt gaagcagcaa tcgtaactga tatcgccgga accacacgtg acgtgctgcg
    27301 tgagcatatc cacattgacg gaatgccgct gcatatcatc gataccgctg ggctacgtga
    27361 agccagtgac gaagtggaac gtattggtat cgagcgcgcg tggcaggaaa ttgaacaggc
    27421 cgaccgcgtg ctgtttatgg tcgatggcac cacaacagac gccgtggatc cggcagagat
    27481 ctggccggaa tttatcgccc gtctgccagc gaaactaccg atcaccgtgg tgcgcaataa
    27541 agccgatatc accggcgaaa cgctgggaat gagtgaagtg aacggtcacg cgttaattcg
    27601 tctctcggca aggacaggtg aaggcgtgga ggtgctgcgt aaccatctca aacagagcat
    27661 gggctttgac accaacatgg aaggcggctt cctggcgcgt cgtcgccacc tacaggcgct
    27721 ggaacaggca gcggagcatt tgcagcaagg taaagcgcaa ctgttaggtg cctgggcggg
    27781 tgaactgctg gcggaagagt tgcgcctggc acagcagaac ttaagcgaaa tcaccgggga
    27841 atttacctct gatgatttgc tggggcggat tttctccagc ttctgtattg gtaagtaacc
    27901 gcgcttacga agccgtattc tgactgtcag atgcggcttc gcttcattgt tagcactcct
    27961 gttattcctc aacccttttt ttaaacatta aaattcttac gtaatttata atctttaaaa
    28021 aaagcattta atattgctcc c
//
LOCUS       DAEEKQ010000038        27882 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.038, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000038 DAEEKQ010000000
VERSION     DAEEKQ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27882
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.038"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            391..2412
                     /gene="uvrB"
                     /locus_tag="KLK57_001591"
     CDS             391..2412
                     /gene="uvrB"
                     /locus_tag="KLK57_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HBE0891983.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(2604..3512)
                     /gene="yvcK"
                     /locus_tag="KLK57_001592"
     CDS             complement(2604..3512)
                     /gene="yvcK"
                     /locus_tag="KLK57_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HBE0891984.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      3777..3921
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            3909..4898
                     /gene="moaA"
                     /locus_tag="KLK57_001593"
     CDS             3909..4898
                     /gene="moaA"
                     /locus_tag="KLK57_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HBE0891985.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEVGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            4920..5432
                     /gene="moaB"
                     /locus_tag="KLK57_001594"
     CDS             4920..5432
                     /gene="moaB"
                     /locus_tag="KLK57_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HBE0891986.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            5435..5920
                     /gene="moaC"
                     /locus_tag="KLK57_001595"
     CDS             5435..5920
                     /gene="moaC"
                     /locus_tag="KLK57_001595"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HBE0891987.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIVGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            5913..6158
                     /gene="moaD"
                     /locus_tag="KLK57_001596"
     CDS             5913..6158
                     /gene="moaD"
                     /locus_tag="KLK57_001596"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HBE0891988.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            6160..6612
                     /gene="moaE"
                     /locus_tag="KLK57_001597"
     CDS             6160..6612
                     /gene="moaE"
                     /locus_tag="KLK57_001597"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HBE0891989.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            6749..7453
                     /locus_tag="KLK57_001598"
     CDS             6749..7453
                     /locus_tag="KLK57_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HBE0891990.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            7658..8371
                     /locus_tag="KLK57_001599"
                     /pseudo
     CDS             7658..8371
                     /locus_tag="KLK57_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
     gene            complement(8436..9147)
                     /locus_tag="KLK57_001600"
                     /pseudo
     CDS             complement(8436..9147)
                     /locus_tag="KLK57_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308893.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
     gene            complement(9147..10388)
                     /gene="clsB"
                     /locus_tag="KLK57_001601"
     CDS             complement(9147..10388)
                     /gene="clsB"
                     /locus_tag="KLK57_001601"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HBE0891991.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGDAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRNFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(10385..11146)
                     /locus_tag="KLK57_001602"
     CDS             complement(10385..11146)
                     /locus_tag="KLK57_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBE0891992.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            11279..11689
                     /locus_tag="KLK57_001603"
     CDS             11279..11689
                     /locus_tag="KLK57_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HBE0891993.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(11651..12757)
                     /locus_tag="KLK57_001604"
     CDS             complement(11651..12757)
                     /locus_tag="KLK57_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0891994.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(12768..13901)
                     /locus_tag="KLK57_001605"
     CDS             complement(12768..13901)
                     /locus_tag="KLK57_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0891995.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(13894..15630)
                     /locus_tag="KLK57_001606"
     CDS             complement(13894..15630)
                     /locus_tag="KLK57_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0891996.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKETTPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKHGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(15623..16618)
                     /gene="hlyD"
                     /locus_tag="KLK57_001607"
     CDS             complement(15623..16618)
                     /gene="hlyD"
                     /locus_tag="KLK57_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HBE0891997.1"
                     /translation="MKKPVVIELAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERYLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(16621..17292)
                     /gene="cecR"
                     /locus_tag="KLK57_001608"
     CDS             complement(16621..17292)
                     /gene="cecR"
                     /locus_tag="KLK57_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HBE0891998.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELLLRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGSDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            17521..18885
                     /gene="rhlE"
                     /locus_tag="KLK57_001609"
     CDS             17521..18885
                     /gene="rhlE"
                     /locus_tag="KLK57_001609"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HBE0891999.1"
                     /translation="MSFDYLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(19117..19599)
                     /gene="ybiA"
                     /locus_tag="KLK57_001610"
     CDS             complement(19117..19599)
                     /gene="ybiA"
                     /locus_tag="KLK57_001610"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HBE0892000.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            19719..21869
                     /gene="dinG"
                     /locus_tag="KLK57_001611"
     CDS             19719..21869
                     /gene="dinG"
                     /locus_tag="KLK57_001611"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HBE0892001.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRVPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTANNQEEQKRCAKLK
                     GDLDTYRWDGLRDHTDIVIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRVE
                     PSIDNEEQHIAEMAAFFRKQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            21897..22859
                     /gene="ybiB"
                     /locus_tag="KLK57_001612"
     CDS             21897..22859
                     /gene="ybiB"
                     /locus_tag="KLK57_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HBE0892002.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGAALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMVCCLVATGEAATISDGLARVNQAF"
     gene            23000..24085
                     /gene="hcxB"
                     /locus_tag="KLK57_001613"
     CDS             23000..24085
                     /gene="hcxB"
                     /locus_tag="KLK57_001613"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HBE0892003.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(24314..24574)
                     /locus_tag="KLK57_001614"
     CDS             complement(24314..24574)
                     /locus_tag="KLK57_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0892004.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPITASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(24839..25105)
                     /gene="ybiI"
                     /locus_tag="KLK57_001615"
     CDS             complement(24839..25105)
                     /gene="ybiI"
                     /locus_tag="KLK57_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HBE0892005.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(25179..25856)
                     /gene="ybiX"
                     /locus_tag="KLK57_001616"
     CDS             complement(25179..25856)
                     /gene="ybiX"
                     /locus_tag="KLK57_001616"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HBE0892006.1"
                     /translation="MMYHIPGVLSPQDVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSQHCV
                     PPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGESEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(25898..27882)
                     /locus_tag="KLK57_001617"
                     /pseudo
     CDS             complement(25898..27882)
                     /locus_tag="KLK57_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         6552 a         7206 c         7514 g         6610 t
ORIGIN      
        1 gtattagagt tagaaaacac gaggcaagcg agagaatacg cggcttgcac gcgaattggc
       61 gttaaagacg gctcaaagaa atatctttta ttttttaact ggttagataa atgcaatggc
      121 agtcactgaa caggcatctc ttgccataaa actgtcatca ctcatcttga caaatgttaa
      181 aaaagccgtt gctttgggga taacccggta aggccggagt tttatctcgc cacagagtaa
      241 attttgctca tgattgacag cggagtttac gctgtatcag aaatattatg gtgatgaact
      301 gtttttttat ccagtataat ttgttggcat aattaagtac gacgagtaaa attacatacc
      361 tgcccgccca actccttcag gtagcgactc atgagtaaac cgttcaaact gaattccgct
      421 tttaaacctt ctggcgatca gccagaggcg attcgacgtc tcgaagaggg gctggaagat
      481 ggcctggcgc accagacgtt acttggcgtg actggctcag ggaaaacctt caccattgcc
      541 aatgtcattg ctgaccttca gcgcccaacc atggtacttg cgcccaacaa aacgctggcg
      601 gcccagctgt atggcgaaat gaaagagttc ttcccggaaa acgcggtgga gtatttcgtc
      661 tcctactacg actactatca gccggaagcc tatgtaccga gttccgacac tttcattgag
      721 aaagatgcct cggttaacga acatattgag cagatgcgtt tatccgccac caaagcgatg
      781 ctggagcggc gtgatgtggt tgtagtggcc tctgtttccg cgatttatgg tctgggcgat
      841 cctgatttat atctcaagat gatgctccat ctcacggtcg gtatgattat cgatcagcgc
      901 gcgattctgc gccgactggc ggagctgcaa tacgctcgta atgatcaagc attccagcgt
      961 ggtactttcc gcgttcgtgg cgaggtgata gatatcttcc cggcagaatc ggatgacatt
     1021 gcacttcgcg tggaactgtt tgacgaggaa gtggaacgat tgtcgttatt tgacccgctg
     1081 accgggcaga ttgtttccac tattccacgt tttaccatct acccgaaaac gcactacgtc
     1141 acaccgcgcg agcgcatcgt acaggcgatg gaggagatca aagaagaact ggccgccaga
     1201 cgcaaagtgc tgttggaaaa caacaaactg ctggaagagc agcggctgac ccagcgtacc
     1261 cagtttgatc tggagatgat gaacgagctg ggctactgtt cggggattga aaactactcg
     1321 cgcttcctct ccggtcgtgg accgggtgag ccaccgccga cgctgtttga ttacctgcct
     1381 gccgatgggc tgctggtggt cgatgaatct cacgtcacca ttccacaaat tggcggcatg
     1441 tatcgcggtg accgggcgcg taaagagaca ctggtggagt acggcttccg cctgccatca
     1501 gcgctggata accgtccgct taagtttgaa gagttcgaag cattagcgcc gcaaaccatc
     1561 tatgtttcgg cgacgccggg taattacgag ctggaaaaat ccggcggcga tgtggtggat
     1621 caggtggtgc gtccaaccgg attgcttgac ccgattatcg aagtgcggcc ggtggcgaca
     1681 caggttgatg atcttctttc ggagattcgt cagcgagcgg caattaacga acgcgtactg
     1741 gtcaccacac tgaccaagcg gatggcggaa gatcttaccg aatatctcga agaacatggc
     1801 gagcgcgtgc gttatcttca ttcagatatc gacaccgtcg aacgtatgga gattatccgc
     1861 gacttgcgtc tgggtgagtt cgacgtgctg gtagggatca acttactgcg cgaaggtctg
     1921 gatatgccgg aagtgtcgct ggtggcgatc ctcgacgctg acaaagaagg cttcctgcgt
     1981 tccgaacgtt cgttgatcca gactattggt cgtgcggcac gtaacgttaa cggtaaagcg
     2041 attctctacg gcgataagat caccccatca atggcgaaag cgattggcga aaccgaacgt
     2101 cgccgtgaga aacagcagaa gtacaacgag gaacacggaa ttacgccgca aggcttgaac
     2161 aagaaagtgg tcgatatcct ggcgctgggg cagaacattg ccaaaaccaa agcgaagggc
     2221 agaggaaaat cgcgcccgat tgttgagccg gataatgtgc cgatggatat gtcgcctaaa
     2281 gcgttgcaac agaaaatcca tgagctggaa gggttaatga tgcaacacgc gcagaatctg
     2341 gagttcgaag aagcggcaca aattcgtgac cagttgcatc agctgcgtga gctgtttatc
     2401 gcggcttcgt gacaggataa cgaagaagac agatgacaaa agcaaaattg cctgatgtgc
     2461 tacgcttatc aggcctacat gatctctgca atacattgaa ttagcgcggt ttgtaggccg
     2521 gataaggcgt tcacgccgca tccggcataa acaacgcgca ctttgtcttc aataggaagc
     2581 cggaattttt cttcggattt ccgttaacct aaagcctgta acgccttttc cagcgcgtta
     2641 tgtaacaact ggcggtcatg acgatacgga atatcgctgg cctccagtac ctcctgaatc
     2701 acaatccgct ctttcaccgc cgagacatcc acttttggcc cgacgatgac cgcatcaatg
     2761 acctttttac caacatactg ctccataatt gccagcttgc tttccagctt caaattagcc
     2821 gcaggtaaac tcaactcacg ccccagattg ccgatataga ccatcggcgc tggcgtgcgg
     2881 cgtaatgcct gggcgatttc cttcagcagc agaattggca tcaggctggt ataaaaactg
     2941 ccaggcccaa taatgatgag atccgcttca ttgatagcgt gaaccgcctc acgcgttgcg
     3001 ggtacattcg gcgttaataa taactcttga atcggcgtag ttaactggtc gatattgacc
     3061 tcgccgtaaa cttcatgccc ctgatcgtca atcgccatca gatcgacggg atgctctgac
     3121 attggaatca aatgcgtatc cactttcagc agattacgaa ttaaattgat ggcttccaga
     3181 ggccgcacgc taaggtgatc cagcgccttt aacatcaagt ttccgagatt atgaccggaa
     3241 agttcgccat tgccgccaaa acggtattca aacatcgcgg aggcgacgct cggttccgtt
     3301 atcagttggt tgaggcagtt gcgcatatcg ccccaggcaa tgccgccttc tgaacggcga
     3361 atacgccccg tcgagccacc attatccgtg gtggtgacga tacccgttaa acgagaaccc
     3421 aaagacgaaa gtgatgagag aacgcgtccc agtccatgcc ctccgccgag agcaacgaca
     3481 cgatcaagat cagccagcgt acgattgcgc atatagtttc ctggagtcag attatccgcg
     3541 ctacagtagc gcaaattgcg gggaaacagc aattaacctg ccaatattga ggatacaaac
     3601 tattttcttc tacctctaaa ggacgatgca cgctatgcct ccctgatgat gtatatcaaa
     3661 gtaaaactgc cattttccct tattctgtag cgaaatagca cgatcatgac gctatataca
     3721 tgattacata gcgaaagtgt ggatggtaaa aatctcattt acacgctagt atcggcttaa
     3781 ccactaaaca ctctagcctc tgcacctggg tcaactgata cggtgctttg gccgtgacaa
     3841 tgctcgtaaa gattgccacc agggcgaagg aagaaatgac ttcgcctccc gtatctggaa
     3901 aggtgtacat ggcttcacaa ctgactgatg catttgcgcg taagttttac tacttgcgcc
     3961 tgtcgattac cgatgtgtgt aactttcgtt gcacctactg cctgccggat ggctacaaac
     4021 cgagcggcgt caccaataaa ggctttctta ccgtcgatga aattcgccgg gttacgcgcg
     4081 ccttcgccag tctgggcacc gaaaaagtgc gcctgacagg aggagagccg tctttacgcc
     4141 gcgactttac cgatatcatc gccgctgtgc gggaaaacga cgctatccgc cagattgcgg
     4201 tcaccaccaa tggttaccgt ctggaacgcg atgtggcgaa ctggcgcgat gcgggactta
     4261 ctggcattaa cgtcagtgtc gacagtctgg acgcccgcca gtttcacgct attaccgggc
     4321 aggataaatt caaccaggtc atggcaggga tcgatgctgc atttgaggtc ggttttgaga
     4381 aggtcaaagt caataccgtg ctgatgcgtg atgttaatca tcaccagctc gacacctttc
     4441 tgaactggat ccagcatcgc cctatccagc tgcgtttcat cgaactgatg gaaacgggcg
     4501 agggcagtga gctcttccgt aagcatcaca tctctggtca ggttctgcgt gacgagctac
     4561 tgcgtcgcgg ctggatccac caattacgtc aacgcagcga cggtcctgcg caagtctttt
     4621 gccatccaga ttacgccgga gagattggcc ttatcatgcc gtatgaaaaa gacttctgcg
     4681 ccacttgcaa ccgcctgcgc gtttcctcca ttggtaaact ccatctctgc ctgtttggtg
     4741 aaggcggcgt taacctgcgc gatctgctgg aagacgatac ccagcaacag gcgctggaag
     4801 cgcgtatttc agcggcgctg cgggagaaga aacagaccca tttcctgcat caaaacaaca
     4861 ccggtattac gcaaaactta tcgtacattg gcggctaaaa cgtcaaaagg agagatcaga
     4921 tgagtcaggt aagcactgaa tttatcccga cccgtattgc tattcttacg gtttctaatc
     4981 gtcgcggtga agaagacgat acctccggtc actatttgcg cgattcggcg caagaagcgg
     5041 gccatcacgt tgtcgataaa gccattgtga aagaaaaccg ctacgctatt cgcgctcagg
     5101 tatctgcgtg gatcgccagc gacgatgtac aagtggtgtt gattacgggg gggactggcc
     5161 tgacggaagg tgatcaggct cccgaagcat tgctgccgtt gttcgaccgt gaagttgaag
     5221 gttttggtga agtgttccgg atgttgtcgt ttgaagagat tggcacttcc acgttgcaat
     5281 ctcgtgcggt agcgggcgtc gccaacaaaa cgctgatttt cgccatgccg ggttcgacca
     5341 aagcgtgccg taccgcatgg gaaaatatca tcgcgccgca gctggatgcc cgtacgcgtc
     5401 cgtgtaattt ccatccacat ttgaagaaat aagtatgtcg caactgaccc atatcaacgc
     5461 cgctggcgaa gcgcacatgg tggatgtctc cgccaaagcg gaaaccgtgc gtgaagcgcg
     5521 ggcggaagcc tttgtcacca tgcgcagcga gacgctggcg atgattattg atggtcgcca
     5581 ccacaaaggc gacgtatttg ccactgcgcg tattgtcggt attcaggcgg caaaacgcac
     5641 ctgggatctg atcccgctgt gtcacccgct gatgctcagc aaagttgaag tcaatttaca
     5701 ggccgagccg gagcacaatc gggtgcgtat agaaacctta tgccgcctga ccgggaaaac
     5761 cggtgtcgaa atggaagcgt taaccgcggc ctccgtggcg gcgctgacca tttatgacat
     5821 gtgcaaagcg gtgcaaaaag atatggtgat tggtccggta cgtttgctgg cgaagagcgg
     5881 cggcaagtcg ggtgacttta aggtggaagc ggatgattaa agttcttttt ttcgcccagg
     5941 tgcgcgagtt ggtgggaaca gatgcaaccg aagtggctgc ggattaccca accgttgaag
     6001 cgttacgcca gcacctggct gcgcagagcg atcgctgggc gctggcgctg gaagatggca
     6061 aattgctggc tgccgtcaac cagacgctgg tgagttttga ccatccgctg actgacggcg
     6121 acgaagtagc tttcttcccg ccggtaaccg gaggttaaga tggcagaaac caaaattgtt
     6181 gtcggcccgc agccgttcag cgtaggagaa gagtacccgt ggctggctga gcgtgacgaa
     6241 gacggtgcag tggtcacgtt taccggtaag gtgcgcaacc ataacctggg tgacagcgtc
     6301 aaagcattaa ccctcgaaca ctatccgggg atgactgaaa aagcactggc agaaatcgtt
     6361 gatgaagcgc gtaaccgctg gccgctgggg cgcgtcacgg tgattcaccg catcggggaa
     6421 ttatggccgg gcgatgaaat cgtttttgtc ggtgtcacca gtgcgcatcg cagcagtgcg
     6481 tttgaagccg ggcagtttat tatggattat ctcaaaaccc gcgcaccgtt ctggaagcgc
     6541 gaagccacgc cggaaggcga ccgctgggtt gaagcccggg agagcgatca gcaggcggca
     6601 aaacgctggt agttttttgt tagccggata aggcaccgct gcgtccggca atttcaccgc
     6661 tcagtacatg cgtttacgct gcgtcgatgc acagcctcat cattttgcag tatccttaag
     6721 atattcctta tatcttcagg agatcgtcat ggacagattc ccacgttctg attcaatcgt
     6781 acaaccccgg gctggcttgc aaacttatat ggcgcaagtc tatggctgga tgaccgttgg
     6841 cttgttgctg accgcatttg ttgcctggta tgcggctaat tccgcggccg tgatggagct
     6901 gttgttcact aaccgtgtct ttttaatcgg tctgatcatc gcgcaattag cattggttat
     6961 tgtgttatca gcgatgattc aaaagctgag cgcaggtgta acgacgatgc tctttatgct
     7021 ttattcggcg ctgacgggtc ttacgctttc cagtatattc attgtctata ccgctgcttc
     7081 tatcgccagt actttcgtcg ttactgccgg gatgttcggc gcaatgagcc tgtacggtta
     7141 caccacgaag cgcgatttaa gtggctttgg caatatgctg tttatggcgt taatcggcat
     7201 tgtgctggca tcgctggtca acttctggct gaaaagcgaa gcattgatgt gggcagttac
     7261 ctacatcggc gtgattgtct ttgtcggatt gacggcgtat gacactcaga aattgaaaaa
     7321 tatgggtgag cagattgata cccgcgacac gtcgaacctg cgcaaatatt ccattcttgg
     7381 cgcgttaacc ttgtatctgg acttcatcaa cctgttcctg atgttgttac ggatcttcgg
     7441 caaccgccgt taattctttt tcaccatcac tatcccggcc atgtgtcggg atttttttct
     7501 tactaaaaac gacacttaac aagtaccagg tataaaatat gccttatgag cgagcggaat
     7561 cgttttccgt atccataaat acaaaagtat tttgtatgcg tggttttaaa tttattgatg
     7621 gtgaatatta atattttttc taaggacaac taaaagaatg gaatcatact cgcaaaacag
     7681 caataaatta gattttcagc acgaggccag gatataaaac ggtatatggc tcattaccgc
     7741 tttaggtttg gtggcaaccg caggactagc ctggggagct aagtatctcg aaattacggc
     7801 aaccaaatat gattcaccac caatgtatgt cgctataggg ttattattac tttgtatgta
     7861 tggcttaagt aaggatatca acaagataaa tgccgccatc gcgggcgtaa tatatctgtt
     7921 tttactctct ttggtggcga ttgtcgttgc aagtttagtt cctgtatatg ccattatcat
     7981 cgtgttcagc actgcgggcg cgatgttttt aatcagtatg ctggccggtt tattatttaa
     8041 tgttgagcct ggttctcacc gttttatcat tatgatgacg ttgacagggt tggccctggt
     8101 aatcatcgtg aatgcggcat taatgagtga acggcccatt tgggtaataa gttgcttaat
     8161 gattgtgtta tggccaggca ttatctcgca tggacgaaat aagctccttg aactggcagg
     8221 aaaatgccat agtgaagagt tgtggagtcc ggttcgttgc gcttttacag gtgcattaac
     8281 actctattac tattttatcg gcttctttgg gatacttgcc gcgatagcta taacgcttgt
     8341 ctggcaaagg catacgcgtt tttttcatta gccgtaaatg cacatactaa agaacttaac
     8401 taaacttcac atcgccgctt catttttcgc ccgtagccca gtacaaagaa atagttcacg
     8461 ctttgaccaa gtaacagcca gataatcgcc cccattacca tccagttaac gctggaaatc
     8521 agcatctgag cgagggcgaa tttccatgaa ggcagcacca gcttttgccc tttgatggtc
     8581 atatggcggt gcttcgcgaa agcgcaaaac cacaaataaa ccgcgataat catcagtaag
     8641 ccaatgccga gaatacgcag cgtagtttga tcgacatacc agtgatccgg caactccacc
     8701 acgcctgcgg taaagataat ccctgccagt aaaatatagc ccagccagtt ggtggtaata
     8761 ctgagcgaga aaatccgcgt aatagtgcta ccgggcaacc ccagacgaga gtacaaacga
     8821 tagcgcatac caatgccgcc gacccaggta ctgagcgtca ggttgaaggc gtagcagata
     8881 aacgacacca gcatcacctg gcgcttcgcc agtttgtgac cgcagtaaaa gcgggcgagc
     8941 aggtcatagc agccgtaaat cagatagctg acgaccacca gcgaccgcac taagcagcgc
     9001 aacgcgattg tagtcgcgga tgaccttcca gacctcttcc cagtccactt ttttggcgta
     9061 gaccaccagc aacacaatca ccgcgataaa aaacagccag gtgaggatct tctttgctaa
     9121 gcgccagcgc gggtgtgatt tactcatcag ggttttaccc ccgtgttttc agtttctacc
     9181 cgatcctgcg tttccattgt cggttgtgcg ggcggatcaa cctgcgccag acgtggcgtg
     9241 tgtgccggaa gccagccaac cagcgccggg aagtggcgta aaaagtggaa cgccagcacg
     9301 cttttggtca ggttccacca ggtgcgtttg ggcagcatgg tttcatccac ctgctgacaa
     9361 tctgcggcga taatgccgtt cagattatcg cgcagcgtct ggttaaaatt acgatcgtgg
     9421 atgatgacat ttgcttcgag attcagtgac aaactgagcg gatcgagatt actggaccct
     9481 actgtcgccc agtgatcgtc catcaatgct actttgccgt ggagcgggcg gcggcggtac
     9541 tcaaacacct gaacgccgcc tttaaccaga tagttataca gcaagcgcgc gccgactctg
     9601 acaatcggca tatccggttc gccctgaatg atcagtttga tccgcacccc gcggcgtgcc
     9661 gctttacgca aggcgtgtaa aaatcgatag ccggggaaga agtaggcgtt ggcgataatg
     9721 acttcccgcc gcgcctgagt gagcattttc agatagtggc gttctatatc atcgcggtgt
     9781 tcttcgttat cgcgccagac cagcaatacc tgcgcgtctc ccggctggcg gttctcttcc
     9841 gctttgtgat gacgtcgcca ccagcgtcgt gccgcgctct gtccaggcag gttttccagc
     9901 tcaaactgga gaatatcttc gacaatcggc ccttcaaggc gtaccgcgta atcctgttta
     9961 gcctctggac cgtagctgga catatgctcg gcggagtaat tcagcccgcc aataaaggcg
    10021 atacgcgcgt cgatcaccac aattttgcga tgcatccggc gaaacacatt ggtgcgcata
    10081 ccaaaaaggc gagggcgggg atcgtagtag cggaacacta cgccagctgc cgtcagttca
    10141 ttgacaaact catcgctgag atccggcgaa ccgtagccat ccagcaagac ttccgcttta
    10201 accccgcgtt gcgctgctgc cagtagtgcc gcatgcagct gtttgccgac gttatcctca
    10261 aaccagataa acgtttcaag aatgatgcgt tcttgtgcct cgccaatcgc cttaaacacc
    10321 gcgggataat attgctcgcc gttttccagc aactggatct tattgccttc gcgccagcta
    10381 catttcataa atgaatctcc gcacttaaag gggcatgatc agaaaggtgt cgccatgtcc
    10441 gcagcggcaa cgcggttggc gcgctggcgc tggcattttt gacgtagatc ctgtccagtc
    10501 gtagtagagg aaattgcacc ggaaacgtgc gcgccgggcg tccgtgggcg cgggtaaaaa
    10561 tctcatccag tccggcctgc acttttaacg gatgattagc tttttgccgc cagtcattga
    10621 aatcacccgc caccaatacc ggttcgccgt ccggtagctc attcacccat tcggcgagca
    10681 tcgcaagctg cgcctgacgg tgcgcctcac gcaggcccag atgtacgcac atcacatgaa
    10741 tcgcttttcc ggtcatcggc ggcacaatgc ggcagtagag cacgccgcgc ttttccgcac
    10801 catcgaccga aacatcgcga ttctcataat gttcaatggg ataacgcgac agtacggcgt
    10861 tgccgtgatg cccttccggg tatacggcat tgcgaccgta ggcaaaatcg ctccacatag
    10921 tgtcggcgag aaactcgtag tgcgaggtat cgggccagtt ttccacatgc agcggatgaa
    10981 cttcgtgcgc gcccatcact tcctgcaggc aaacaatatc ggcgctgacg gtacgcacgg
    11041 catcgcgaag ttccggcaaa atgaagcgtc ggttaaacgc ggtaaagcct ttgtgaatgt
    11101 tgatggtgag caccttgaac gaaaattgtt gtgtttgatc gggcataatt ttcctgtctt
    11161 tgcctcttat ctcattgaaa tagtgtagtc ggcgtcacaa aaaggtgcgg tcttacggaa
    11221 ttttccgtaa agttcggtac tctgagtaag tagagataaa ttcttcagga gagaagccat
    11281 gaagtggcaa caacgtgttc gtgtcgcaac gggtctaagt tgctggcaga ttatgttgca
    11341 tttactggta gtggcgctgc tggtggtggg ctggatgagt aagactctgg ttcacgtcgg
    11401 cgtgggatta tgcgcactgt attgtgtcac ggtagtgatg atgctggtgt ttcagcgcca
    11461 ccccgagcaa cgctggcgtg aggtggcaga cgtgctggaa gagctgacca cgacctggta
    11521 ttttggcgca gcgctgattg tgctgtggct gttgtcccgc gtactggaaa acaacttttt
    11581 gctggcaatt gcagggctgg cgatccttgc tggcccggcg gtagtgtctt tgctggcgaa
    11641 agataagaag ttacatcacc ttacgtctaa acatcgcgta cgccgctgac cctgtcgtgg
    11701 ccgttatcac cagtagcggc cacaaactat tccacacaat atccagactc gcatccttca
    11761 aataaatctg cttggtaatg tccgtaaagt ggcgaatagg gttaatccac gtcaggtttt
    11821 gcagccatac tggcatgttt tccaccggag aaacgtaacc ggaaaggaga atggcgggca
    11881 tcataaaaac aaacacgccg ataaacgcct gctgttgtgt cgagcagagt gatgaaatca
    11941 acagaccgaa tcccaccagc gataaaccgt aaatcaccat cgtaaagtag aacagcgcca
    12001 gcgatccggc gaaggggatt tgatacgccc agataccaat cgccagcaca atggtggcct
    12061 ggaaggtggc gacaattaac gccggtacgg ctttgccgat gaagatctgc caggtggtga
    12121 gcggcgaaac cagtagctga tcgagcgtac cttgttcacg ttcgcgggcg acggaaagtg
    12181 aagtgacgat cattacgccg atagtggtga tcatggcgat cagcgacggc accacaaacc
    12241 atttgtagtc aagattcggg ttataccagt tgcgtaccac cagctcgctg ttgttaggtt
    12301 tcggttttcc ttccagcagc tcctgctgat aatttttgac gatctgttgc aggtagttgg
    12361 cggcaatttg cgcactgttg gagttacgcc cgtcgaggat caactgcaac ggcgcggtct
    12421 ggaaggtatc cagtttgcgc gagaagtcag ccgggaaacg caccagtagt aacgcctttt
    12481 gtgtgtcgat ggttgggcgg atctcctgtg ggcttttcag cagcagcaca tgggtaaagg
    12541 cgctggcgcg ggcaaaacgt tgggtcagct ccaccgaatg ctcgccgtta tcttcatcgt
    12601 agatggcgat ggtggcgtta gtcacttcca gcgtggcggc gaacgggaac aggatcacct
    12661 gaataagcac aggtaaaatc agaatcgcgc gggtttgtgg ttcgcgcagc aacgactgca
    12721 actctttgcg gattaacgtc cataagcgat gaaacatgct cttctcccta atccagccga
    12781 cgtttggttt tcagccacgt caggccgata aacatcaccg ccgaagcgat caaaaacagc
    12841 acgtttacca ccagcaccac tggaatattc ccggcgagga acaggctttg cagggtgctg
    12901 acgaaataac gagcgggaat aatgtacgtc accgcgcgga tcaccgcggg catactgtcg
    12961 atctgaaaaa taaagccgga aagcataatc gacggcagga acgcggcgtt cagggcgacc
    13021 tgagcggcat tgaactggtt gcgggtaatc gtggaaatca gcagccccat ccccagggta
    13081 ctgagtaaaa acaggctgga gataaaaaac agaataagca gcgaaccgcg atacggcacg
    13141 ccgagaataa acactgacac cagcatacac agcagcatcg ccagcatccc gagaaagtaa
    13201 taagggatca gcttacacag cagcagttcc gtgcgggtaa tctccgtaga gagcagagcc
    13261 tccatggtgc cgcgttccca ttctcgcgcc accaccagcg aggtgagaat cgcgccgatg
    13321 accgtcatga tgatggtcac tgcaccgggg ataatgaagt gctggctaat cgccgccggg
    13381 ttaaaccagt agcgggtttg tacatcaata agcggttcaa aagtctgccc gttgtcctcc
    13441 gctcgctgca tttgccagat ctgccagatc ccttcgacat acccctgtac aaagttagcg
    13501 gtattcggct cactgccgtc ggtgatcacc tgaatcggtg cggtggcgtt ggcgcgctcc
    13561 atctgttccg caaaatccac cggaataacc accagaccgc gaattttccc cgcctgcatt
    13621 ttggcgatca gttcctgacg gttatcgctg atggtggcgt cgatgtaggg cgaaccggtc
    13681 atggtgtggg tgaaatccag cgccgcttcg ctacgctgtt ccagcaagat cccgacccgc
    13741 agcttgctgg agtcgaggtt aatgccataa ccaaaaataa acagcagtag cagtgggatc
    13801 actaccgcaa tcagccagct actcggatcg cgaacgatct gccgcgtctc tttgacgcac
    13861 agcgcccgca cgcgacgcca ggaaaggatc gggttactca ttgctatgct ccttatccca
    13921 gtcgtggatc aactgaataa aggcttgctc catggtggga tcgggttgct catcgttagc
    13981 cgactgtgct ttcaaatcgt ccggcgtacc gctggcgatt aatttcccac ggtacaccag
    14041 gccgatgcgg tcgcaatatt ccgcttcatc cataaagtgg gtggtgacca tcaccgtgac
    14101 gcctttctct accatgctgt tgatatgtag ccaaaattca cggcgggtga ggggatcaac
    14161 gccggacgtc ggttcgtcga gaaacagaat gtccggttca tgcatcagcg aacaggccag
    14221 cgccagccgc tgtttaaaac ctaatggcag ttcatcggtg gcgtgggagg cgatactttt
    14281 caggccgaac gcctcgctca tgcgggagat tttttcgttc tgcgcccgac cgcgtaagcc
    14341 atacacacca gagaaaaagc gtaaattctg ttcgaccgtc aggttaccgt agagcgaaaa
    14401 cttttgcgcc atatagccga gatgctggcg cgctttacca gaactctctt tcagatccat
    14461 ccccagtacc agcgcctgac cagaggtcgg caccagcaaa ccgcacatca tcttaaaggt
    14521 ggtcgatttg cccgcgccgt ttggccccag caaaccaaaa atctccccgt gtttaacggc
    14581 aaagttgacg tgatcggtgg cggcaaaatc accaaatttc ttggtcagtt ctttcgcttc
    14641 gatcaccgtc tcgccgggtg tgccttctac cgtatgtaat attgcgccca gcggcgattc
    14701 cgaggttccg gcaccgccca gcaaatcaat aaacgcatct tcaaaacgcg gcgtagtttc
    14761 gttgatgttg atttccggca tcccgtcggc atggcgaata tcgtctggtg tggtctcttt
    14821 tttgaggatc agacgtaccg atttcccctg aatcatgccg tcgctgacct gcggcagttt
    14881 caatgcccgt tgcaacagtt tgcggttgcc ctcgtgtgga cttgtcatca gaaagctgcg
    14941 tccggccatg gtttgtgtca gggcttttgg ttccccctga tacagcaact cgccttcatt
    15001 catcagtaac acgtcacggc actgctcggc ttcgtcgaga tacgaggtac tccagaggat
    15061 taacatccct tcgcctgcca gctcatgcac catctgccac agttcgcgcc gtgagatagg
    15121 gtcaacgccg acgccgggtt catcgagcag caacactttc ggttcgccca ccagggtaca
    15181 ggccaggccg agtttttgtt tcatcccacc ggagagcttg cccgccaggc gtccggtaaa
    15241 cggcccaaga gacgtaaact ccagcaggcg agcaaaagtt tgcttacgtg cctcgccggt
    15301 gacgctgcgc aaatccgcgt acagattgag gttctccatc accgtgagat cttcatacag
    15361 accaaatttc tgcggcatat agccgagcac ggcgtgcagc gcgccgtcgt ttttgattgg
    15421 atcaaagcca atcaccgtgg cattgccgct gtcgggtttc agtaatcccg ccagcatccg
    15481 cattagcgtg gttttacctg caccatccgg ccccaccagc cccgtcacat aaccggcgtg
    15541 aatggtacaa tcgagcggcg cgacggcggg cttgtccatg ccaggaaagc gtttttccag
    15601 gccgttcagc gtgataacgg catcattcat gtcctgcctc gttaccgaat tgcaccgtca
    15661 ccggcattcc ctggcgtaac gcatcatcgg cgtcggtcac cacaatacgc aggcgataga
    15721 cgaggtcggt acgcagatcc ggtgtttcga cggttttcgg ggtaaattca gcagtcggcg
    15781 aaacgaaacc aatctgcccg tgatacggct tgtccgggcg accatcggta taaagcagca
    15841 ctttgcgccc cggctgggcc tggtcaagat accgttcatc aacataagcg cgcacccaca
    15901 ccggacgcgt tagtgaaacg gtaaacaccg tgccaccttc attgaggacc gtgcctggct
    15961 ccaccgcgcg cgttaacagc gtgccatcag acggggctat caacgttgag tcctgtaaat
    16021 tcaactccgc ctgcgccagt tgcgcctgcg cctgttcgag gctggctttc gcctgggcga
    16081 tgtcctgttc acggttaccg gaacggtact gacgcaattt atcctgtgct gatttcagcg
    16141 ttgcctgcgc ctggtcgcgc gaggagcggg cattttccag gtcatttgcc gaaatagtgc
    16201 ggcttttcca cagtccttgc tggcggttat agaagttctg cgcatagtca taggcggctt
    16261 gcgcctgttt caccgctgcg gcggcctgag cgatttcttc atcgcgatac ccggcaagca
    16321 tcagatcata ctgcgcctgt gccaccgaga cgcccgcttt cgcctgcatc agggcaatct
    16381 catacggctt gtgatccagt tcgcccagca cctggcccgc tttgatagca tcaccttcgt
    16441 ccaccgccag cgattcaacg cgccccccaa cacggaaact aagattcacc gtacgaatat
    16501 ccacgttgcc atacagcgtc aggccgttat cctggcggct ttgataccac cagtagcctc
    16561 cggcaaccac ggcggcaagt accactaccg ccagttcgat cacgacaggt tttttcatca
    16621 ctacaaactc ctttgcgata atccttgcaa aatcaggtcg atatgacagg tcaccgtctg
    16681 gttgatcagt tcggtctttt cttcatcgaa cgcggtccag ccggtacgta acagaattgt
    16741 ttctttgcca agacggaacg ccagaatctc accaatcaac gcatgggtat gaaggatcat
    16801 gcgggtgtca ttggcgtcgc tgccggtcca ggcggcaatc aggcgtgtca ggtggctgtg
    16861 tagcggacta atcacctgtt cgtgcaccag gtggtaggct gccgtgggag agagctgctc
    16921 acgggagata aacttgctga ggttgacggt gtcatcctgg gtgagcagct taatcatgtt
    16981 cctgcaggcg cgaaggagca gttcacggat ggcagcccga tcaggctgtg gttgggcgaa
    17041 caagcgttcg gcttcctcgg catgcggacg gaactgctcg ccaataaaat cggcaatcca
    17101 ctgggcgcag gcgaggtaca aatcttcttt cgaaccgaag tagtaggtga tggcagcgat
    17161 attctgcccg gcttgggcgg ctatctcgcg ggtggtggcg ttcattccat attcaccaaa
    17221 ctgcgccagt gcggcagcaa tcagctgttt tttcgcctgt tcacccttga ttgtcatggc
    17281 aggattattc atcgcacagt ccattcttaa tcaaatgatt gattaagatt atgactccat
    17341 atgggagttg tccagtatgg ctaagaattt tagctacgcc agtcacaggg ataatttatg
    17401 cgctgcgtca caaaaactgc tacactccgc tcgctcatga cattgtggtt tttgtcattt
    17461 tccttttcag tatctccctg aaaactacac cggtaacggt cggggcggtt cggagtagtc
    17521 atgtctttcg attatttggg tttaagccct gatatcctgc gcgccgttgc cgagcagggt
    17581 taccgtgaac ccacccctat tcagcagcag gcgatccctg cggtgctgga aggccgcgac
    17641 ctgatggcta gcgcccagac cggcaccggc aaaacagcgg gctttacgct gccgctgttg
    17701 caacacctga tcactcgcca gccgcacgcc aaagggcgtc gtccggtacg tgcgctcatt
    17761 cttaccccga cccgtgaact ggcggcgcag attggcgaaa acgtccgtga ttacagcaaa
    17821 tacctgaaca ttcgttcgct ggtggtgttt ggtggtgtga gtattaaccc gcagatgatg
    17881 aaactgcgtg gcggcgttga tgtgctggtg gcaacgccgg gacgtttgct ggacctggaa
    17941 catcagaatg cagtgaagct ggatcaggtt gaaatcctcg tcctcgatga agctgaccgc
    18001 atgctcgaca tgggctttat ccacgatatc cgtcgcgtgt taacaaaact acctgcgaag
    18061 cgccagaacc tgttattctc cgcgaccttc tctgacgata ttaaagccct ggcggaaaaa
    18121 ctgttgcaca acccgctgga aatcgaagtg gcacgccgca ataccgcgtc tgatcaggtg
    18181 acccagcacg ttcactttgt cgataagaaa cgcaaacgcg aattgctgtc gcacatgatt
    18241 gggaaaggga actggcagca ggtgctggtg tttacccgta ccaaacacgg cgctaaccat
    18301 ctggctgaac agctcaataa agatggcatc cgtagtgcgg cgatccacgg caataaatcg
    18361 caaggtgcgc gtactcgtgc gctggctgat tttaaatcgg gcgatattcg tgtactggtg
    18421 gcaactgaca tcgctgcgcg cggcctggat attgaagagc tgccgcacgt cgtcaactat
    18481 gaactgccaa acgtaccgga agattatgtc catcgtatcg ggcgtaccgg tcgtgcggct
    18541 gctaccggtg aagcgttgtc gctggtgtgt gttgatgaac acaaactgct gcgtgatatc
    18601 gaaaaactgc tgaaaaaaga gatcccgcgc attgcgattc cgggctatga gccggacccg
    18661 tcaatcaaag ccgaaccgat ccagaacggt cgccagcaac gtggcggcgg cggtcgtggg
    18721 caaggtggtg gtcgcggtca acagcaacca cgccgtgggg aaggtggcgc aaaatctgca
    18781 agcgcgaaac ctgcagaaaa accgtctcgc cgcctcggcg atgccaaacc ggcaggcgaa
    18841 caacaacgtc gccgccgtcc gcgtaaacct gccgctgcgc agtaatcttt tatgccgggc
    18901 tatgcccggc atcaggctga tgaacaaacg caaaactgcc tgatgcgcta cgcttatcag
    18961 gcctacgtga actctgcaat atattgaatt tgcatgcttt tgtaggccgg ataaggcgtt
    19021 tacgccgcat ccggcatttc acaacaagca cttgtcagca atttgagaac acgggaaaaa
    19081 atttatctgt tttaccgccc ataagccacc gagacgttac ttctctatag ccaattgttc
    19141 tcgcaactcc attaaaaggt agcccagtcg attcttgccc ttaccattac caccgtctcc
    19201 ccagtaagca tcgttttccg tatgctcaac cagttttgcg ggcgcggttg ccagcaagag
    19261 cacacgcagt tctgcatgct gttcgaattt agcacgaagt gctttacgca tcacctgttc
    19321 tttaaccgac tcccagtttt tacgaagtgg cttggaacga ttacgcccca tgcgagcggc
    19381 gaccatgggg gaagaaaccc gacggatctc ttcgcggtat ttttcatcaa ggaatttttg
    19441 tgcctgaaaa tagtgttcgg aggtgggcca ggttttcccg tcaaccttga tgggccaggc
    19501 ggcaaagtta gaaaaatcac cgtagtcatc gctggtgctg tagaaattta tgatggtgtc
    19561 ttgcatgacg tgttggatcc tttgtgctcg aacgggcatt aaaccgcatt atgttggtgg
    19621 ttattgcgag ccgctttcca gaaacagaaa aaccattacc cctgaaaacc gaaaaatgcc
    19681 acaatattgg ctgtttatac agtatttcag gttttctcat ggcattaacc gccgcgctta
    19741 aagcgcaaat tgccgcctgg tataaggcgc ttcaggaaca gatccccgac tttattcccc
    19801 gtgtgccgca gcggcagatg attgcggacg tcgccaaaac gctggccgga gaagaaggcc
    19861 ggcatctggc gattgaagcc cccaccggcg ttgggaaaac gctctcctat ttgatccccg
    19921 gcatcgccat tgcccgcgaa gagcaaaaaa cgctggtggt gagtaccgcc aacgtggcgt
    19981 tgcaggatca gatttacagc aaagatttac cgctgctgaa aaagatcatt cccgatctta
    20041 aattcactgc cgctttcggg cgtgggcgct acgtttgtcc gcgtaatctg acggcgctcg
    20101 ccagcactga acccacgcaa caggatctgc tggcgtttct tgacgacgaa ctgacggcaa
    20161 ataatcagga agagcaaaaa cgttgtgcga agctgaaggg cgatctcgac acttatagat
    20221 gggatggtct gcgcgatcat acggatatcg ttatagatga tgatctctgg cgtcgtttga
    20281 gtaccgacaa agccagctgc ctcaaccgta actgttacta ctatcgtgaa tgcccgtttt
    20341 ttgttgctcg tcgggagatt caggaagcgg aagtggtggt ggcaaaccat gcgctggtga
    20401 tggcggcgat ggaaagcgaa gccgtactgc ctgacccgaa aaatctgctg ctggtgctgg
    20461 acgaaggtca tcacctgcca gatgtggccc gggatgcgct ggagatgagc gctgaaatca
    20521 ccgcgccctg gtatcgacta cagctggact tgttcacgaa actggtcgct acctgcatgg
    20581 agcagtttcg cccgaagacc atcccaccgc tggcgatccc tgaacgtttg aatgcgcatt
    20641 gtgaagagtt gtatgagctt atcgcctcgt taaacaacat tctcaatctc tacatgcctg
    20701 ccgggcagga ggcagagcac cgttttgcga tgggcgaact gcctgatgaa gtgctggaga
    20761 tctgccagcg gctggcaaaa ctcaccgaga tgctgcgtgg tctggcggag ttatttctta
    20821 acgatttaag tgagaaaacc ggcagccatg acattgtacg cctgcatcgg ttgattttgc
    20881 agatgaaccg cgcgttgggg atgttcgagg cgcaaagcaa actctggcgg ctggcgtctc
    20941 tggcgcaatc ttccggtgcg ccggtgacca aatgggcgac gcgggaagag cgcgaagggc
    21001 aactgcatct ctggtttcac tgcgtgggga tacgtgtcag cgatcagctg gaaaggctgc
    21061 tgtggcgcag tattccgcac attattgtca cctccgcaac cttgcgttcg ctgaatagtt
    21121 tttcgcgttt gcaggagatg agtggtctga aagagaaagc gggcgaccgt tttgtggcgc
    21181 tggattcccc ctttaaccac tgcgaacagg gcaaaattgt tattccccgg atgcgcgttg
    21241 agccttccat cgacaacgaa gagcagcata ttgccgaaat ggcggccttt ttccgtaagc
    21301 aggtggagag caaaaaacat ctcggtatgt tggtactgtt tgccagcgga cgggcgatgc
    21361 agcgctttct cgactatgtg acggatttac gtctgatgtt gctggttcag ggcgatcagc
    21421 cgcgttaccg tttagttgaa ctgcaccgca aacgcgtcgc caacggtgag cgcagcgtgc
    21481 tggtgggctt acagtcattt gccgaagggc ttgatttgaa aggtgatctg ctcagccagg
    21541 tgcatatcca caaaatcgct tttccgccca tcgacagccc cgtggtgatc accgaagggg
    21601 aatggctgaa aagcctcaac cgctatccgt ttgaggtgca aagcctgccg agcgcctcgt
    21661 ttaacctgat tcagcaggtt gggcgactga ttcgaagcca cggttgctgg ggcgaagtgg
    21721 ttatctacga caaacgcttg ctgaccaaaa attatggcaa gcgactactg gatgcattac
    21781 cggtatttcc gatagagcaa ccggaagtcc ctgaaggtat agttaaaaag aaagaaaaaa
    21841 cgaaatcccc acgccgtcgg cggcgttaat gatgtgagtc aggtaaggag tcgtaaatgg
    21901 actatcgcaa aatcattaaa gagatcgggc gcgggaaaaa ccacgcgcgc gatttagacc
    21961 gggatactgc gcgcggtctg tatgctcata tgctcaatgg tgaagtccct gaccttgagt
    22021 tgggcggcgt attgattgcg ctgcgtatca aaggagaagg ggaagcagag atgctcggct
    22081 tttacgaagc catgcaaaat cacaccatca agctgacgcc gccagcaggc aagccgatgc
    22141 cgattgtcat ccccagttac aacggcgcgc gtaaacaggc caacctgacg ccgttgttgg
    22201 cgattctcct gcacaaactc ggttttcctg tggtggttca cggggttagc gaagatccaa
    22261 cccgcgtgct gactgaaacc atttttgaat tgatgggtat tacgccaacg ttgcacggcg
    22321 ggcaggcgca ggcgaagctc gacgaacatc aaccggtgtt tatgccagtc ggcgcgtttt
    22381 gcccgccgct ggaaaaacaa ctggcgatgc gctggcggat gggcgtgcgt aacagtgcac
    22441 ataccctggc gaaactggcg acgccatttg ccgaaggtgc ggcgctgcgt ctttccagcg
    22501 tttcgcatcc ggaatatatt ggacgcgtcg cgaagttctt tagcgatatc ggcgggcggg
    22561 cgctactaat gcatggtact gaaggtgaag tgtatgctaa tccgcagcgc tgcccgcaga
    22621 tcaatctcat tgaccgtgaa gggatgcggg tgctgtatga aaaacaggac actgctggta
    22681 gcgagttact gccacaagca aaagatccgg aaaccacggc gcagtggatt gagcgttgcc
    22741 ttgctggcag cgaaccgatt cccgaatcgc tgaaaatcca gatggtttgc tgcctggtgg
    22801 ctacgggtga agcggcaact atcagcgacg gcctggcgcg cgttaatcag gcattttaat
    22861 tcttttctca gccggatgag ccatgtcatc cggcttttac cccaccgcaa tatgaaattc
    22921 ctgcatcttt attgaccttc ccacgcccgg cgtgcagcat aaaaatacaa caaacacata
    22981 acataaacag gagttaacca tggaaagtgg tcatcgcttt gatgctcaga cgctgcacag
    23041 ttttattcag gctgtatttc gtcagatggg tagcgaggaa caagaagcga aattagttgc
    23101 cgatcattta atcgcggcaa acctggcagg gcatgattca catggtattg gcatgatccc
    23161 aagctatgta cgctcctgga gtcaggggca cctgcaaatt aaccatcatg ccaaaaccgt
    23221 taaagaggcg ggggcggcgg tcacgctcga tggcgatcgc gcatttggtc aggtcgcggc
    23281 acatgaagcg atggcgctgg ggattgagaa agcgcatcag cacggtattg ccgccgtggc
    23341 gctacataac tcgcatcata tcggccgtat cggttactgg gcggagcagt gtgcagcggc
    23401 ggggtttgtc tctatccact ttgttagcgt ggtcggtatt ccaatggtcg cgccgttcca
    23461 cggtcgcgac agccgctttg gcaccaatcc gttctgtgtg gttttccctc gtaaagataa
    23521 tttcccgctg ttgcttgatt acgccaccag cgccattgca tttggcaaaa cccgcgtcgc
    23581 ctggcataaa ggcgtccccg tgccgccagg ttgcctgatt gacgttaacg gcgtgccgac
    23641 gaccaatccg gcggtaatgc aggagtcgcc gttgggttcg ctgttgacct ttgccgaaca
    23701 taaaggctac gcccttgcag cgatgtgtga aattcttggc ggggcgcttt ccggcggtaa
    23761 aacgacgcat caggaaacgt tacaaaccag tcccgatgcc attcttaact gcatgaccac
    23821 tatcatcatc aacccggaac tcttcggcgc gccggattgt aacgcgcaga ccgaagcctt
    23881 tgccgagtgg gtgaaagcct cgccacatga tgatgataag ccgattttgc taccgggcga
    23941 gtgggaagtg aacacgcgtc gcgaacggca gaagcagggg attccactgg atgcgggaag
    24001 ctggcaggcc atttgtgatg cagcgcggca gattggtatg ccggaagaga cgttgcaggc
    24061 tttctgtcag cagttagcca gctaaaaaaa agcccgtcca gtggcggacg ggcaaacaag
    24121 ggtaacatag gatcaatgag ggttagagca tatgcgtctg tcggcaaaca gacagggaaa
    24181 tacttgtgct ggacgtagcg taaacgcctg atccgtccaa cggttcggat tttgtaggcc
    24241 tgataagacg cggtagcgtc gcatcaggca tctgttgccg gatgcggcgt aaacgcctta
    24301 tccgtcctac gaattactta taaattaccg cagtaccgct taatttattg ttgttggtgg
    24361 cggaagtgat gctgtatcca ctggcaccgg ctgccgcggc tttctcagcc agtttcgctt
    24421 ccagggcatc gagggtggat gcgccatcgg cagaaaccac gccgatttta ttcatgttct
    24481 gtgcctggga tgccgttatc ggttccgccg cgaacacgcc aaatgacaga gttgaaagag
    24541 ccatagcagc aacaacagta ttgatagttt tcataattaa tctctcgcag gtgatttttg
    24601 tataagtgac gttgtttcgt cgatgtgatg agtatcacgt ttttttacga gagataaaat
    24661 cgaagagaat tgaccgcctt gttcaaataa attgattgat atctaacata ctgataaacc
    24721 atttatttgt tgtacttatg aacttgtttt ataccgcgtg gcagtcacag cagcgttaaa
    24781 ggcacggaat gccacgcaaa ataaaaatta tactcgctat gcatctcgcc agttgtagtt
    24841 aacgtaactg gctgtctttc gaacctctgc gattatatcc tgtataagct ggtttttgta
    24901 aatctttctc ctgctgacaa tgaatacata agcgcacgcc aggaatggct tcccgacggg
    24961 cctgcgggat gggggcaccg cactcttcac attcatccag gctttcgccg cgcggaattt
    25021 caccccgagc gcgggcaatc gcatcttcaa ttgtactgtt gatctgttcg ttgacggcgt
    25081 catcgttagc ccaaccggat gccatatcga cctccccata tcaatacttg tacagttaag
    25141 tgtagctaat ccagggacga actcgggcag ttcaagcatc agatctccga ccattcccgc
    25201 agcagattat gataaagatt aagcagcgac aggatctctt cactttcgcc gtagcggctt
    25261 ttcagcgact gaatattgtt gtccagttca aacagcatgg cgcgcttttt atcatcgcgg
    25321 atcatcgact ggatccacat aaatgatgcc actcgtacgc cgcgggttac gggtggcacg
    25381 caatgctggc tgctggaggg atacaacacg agatcgcctg ccgggagttt tacccgatgt
    25441 tgtccgaagg tgtcattaac gaccagttcg ccgccgtcgt agctttgtgg atcgcttaaa
    25501 aacagcgtgg cagaaaggtc agtacgcatc cagccgtttt gtggatggct gcgtactgcg
    25561 ccatccacat ggaaaccata ggtttcattg ttctgatagc gattaaacag cggcgtggaa
    25621 agggtacgcg gcaaggccgc ggcaaagaac aaagcatgtt ggttaaccgc gttcagcacc
    25681 tcattttgca gggcggcgta taacgtgctg cgggtgtcga cctgttgatt gttcttaact
    25741 tgcgcacctt gtgcgccggt ggtgacgcgt ccatccaccc attcggcttg ttccagttgt
    25801 tcgcgaaacc gggcgacgtc ctgtggcgat aacacgccgg gaatgtggta catcatacaa
    25861 gttctccaaa aagtggggcc tgcgccccac atctgaatca gaaatgcata ttggctgtga
    25921 gcaagaaggt tcttggctcg cccgggtgat aacggtagcc gctcttgttg attgaggcga
    25981 cgtaatcagt atcaaacagg ttgtagacgt ttagctggaa gtcgagattg cgattaaccc
    26041 gataccccag tttggcatcg gcgacccagt aaccttcggt aaatgctggc gttcccaccg
    26101 cgccgtctga acctttatgc atactgccga tatagcgtgc gcccgcgcca acagagatat
    26161 cgtcggttgc ctgatattgg ctccataagg tgaaggcgtg ctccggggta tacggcagcg
    26221 atgaggaacc atcctgggca acatctttgc cgtttttgat ggttgctttt tgctgggtat
    26281 agccgccaat cacctgccac gcgggagtga tattcccggc cacggatatc tcatagcctt
    26341 cgacgcgttt cttaccgtat tgcgagtaag ttccgtcatc attttgctca acttcatttt
    26401 cgatatcagt acggaacagc gcggcggtga gcaacagacg tttatccaga acctgccatt
    26461 tggtgccaat ctcgctggtg ttggcttttt gcggtttaaa atcggtgcgg ttggcactgt
    26521 taccgctgcc agactgcgca agggcgaagt tgttgccgcc cggaggctgc tgggaaacgg
    26581 catagttaat atagacattg ccgttttccg tcaggtgata cagcgccccg gctttccagt
    26641 tcatcagatt gcccgacttg gcggtgtcga cggtggtgac cggagaacct tttgccacac
    26701 cagttgggca ggtgatggca ccgcgtccgc tgccgccgca ggcggtggca ctgtcatatt
    26761 cagtatgata attatccaga cggatcccgc cgttcagctc aaaatcacgg gtgatttgca
    26821 gcgtatcaaa ggcgtaaatt gcgaaggtat ccgtctgacc attggcgttt gcgccgttgc
    26881 gcgtcaggcc gccgggatga atgctgctgt caggatgata aatatttacc gcgggtaacg
    26941 tcaccggatt aacgccgtag ttagtctgcg tttcacgggt aaattccacg ccggtactga
    27001 catcatgacc gatagaaccg gtatagaacg tcgaggtcag gttggtctgg ttggtgagaa
    27061 ttttattact cacatctttg gtattcgccg tgcgtgacca ggttcagcta ttcacatcgc
    27121 tggtgggctg agtaatattc gacgccccgc ccataatcgc cgtcatcagg taatcctgct
    27181 ttacgcgcga ccaacgggta gtattgcgaa tggtggtgtt atcgttgatg tcgtgctcaa
    27241 aacgcatggt ggcggtgtcg gtggtcgaat cgtcgtaatc ggaatccgtg ccgtaaaagt
    27301 tatgagtatc aacttttccg gaatgattca gggccgccgt tcccgcagat ggggcagaat
    27361 agcccggcaa accgatggtc ggaatgccgc cgtctggcgt gttgtgctgg gtgacatgca
    27421 gataattaag atacaaacga ttcgctgtac caaggccaaa agcgacagaa ggggcgacgc
    27481 cgtaacgctc atttttgact ttgtcgcgtc cggcatcgtg cgttttttcg cccattacat
    27541 tcaggcgcac cgcggtggta tcaccaatga cctgattgac gtccagcgtg ccgcggcgga
    27601 accaggcgct gccaatactg gcggaggcgt caatgccgga atcattgcgc ggctgtttgc
    27661 tgatcatatt gatcgagccc gtcggtgcgc tgcgcccgta gtcggtgccg gacggccctt
    27721 taatcacttc gacctgctcg gtattgaagg tgtcgcgcga gacgctgccg atatcgcgaa
    27781 tgccatcaat ataaatactg ttagaggtat cggcaccacg catataaatg gcgtcgccag
    27841 tggtggagtt accgttctca cccgcaaaaa acgcacccac gc
//
LOCUS       DAEEKQ010000039        27353 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.039, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000039 DAEEKQ010000000
VERSION     DAEEKQ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27353
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.039"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(213..386)
                     /locus_tag="KLK57_001618"
     CDS             complement(213..386)
                     /locus_tag="KLK57_001618"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892007.1"
                     /translation="MMIYMVDPVLLKAHNGLRIQMNGTTPDIARVQVINTTHSNFLLL
                     TKLCTLLIDKSGR"
     gene            479..2392
                     /locus_tag="KLK57_001619"
     CDS             479..2392
                     /locus_tag="KLK57_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014883662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBE0892008.1"
                     /translation="MNHWYTIKAADVRGAADISIYEEIGGFGVTAKQFAEDLKALGDV
                     SHINLRIHSPGGDVFEGIAIYNLLRNHPADITVYIDGVAASMASVVAMAGDRVVMPEN
                     AMMMIHKPWGISGGNAGDMRDYADLLDKVETVLIPAYARKTGKSAQEITAMLEDETWM
                     DGKECLKHGFADELLPSVRAMARIESKRTGDFLHMPETIKGMITPPQGAANIAGNEQK
                     RINGISEVFSLFGSRYDGIKMACLEDASCTPEMAREKLLNELGRESTPSNKNTPPHIY
                     AGNGNITGDAIRQGLYSRLGYERPERGNPYAMMSLFEMAQASLVDRGITVSGFINRSQ
                     VVNAAFTHSSSDFSHILAGGAEKSVLKGWQDSGETFQKWTRTGSLSNFHEAKRVGLNG
                     FSKLDKVPEGAEYKYITTSDKGVPIALATYGNIFSVTRQAIINDDLTQLTTIPMAMGR
                     AAARTVGNLVYLLLTSNGKFTDGKALFHADHKNLIAKDMDMEGLNEARKLMRLQEDAN
                     GDSLNITPAFVLVPAALESAAHRAILSSSSLFPVDGVGTINQNPGIINVVKDMAEVIV
                     EPRLDKANNKEWYVAAAKGMDTIEVAYLDGIDTPYLEEQEGFTVDGVAWKVRIDAGVA
                     ALDYRGLLKSSGA"
     gene            2650..3135
                     /locus_tag="KLK57_001620"
     CDS             2650..3135
                     /locus_tag="KLK57_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1441 family protein"
                     /protein_id="HBE0892009.1"
                     /translation="MDRELKNLMLNINQLAAIAGICRQTAAARLKNIQPAGGHDKLKL
                     YRVTDILTCFLDLPVPASLEEMEPHDRKAWYQSERERLKFEQETAQLIPADDVRKEMA
                     IWGEIVSEELAKLPNILARDAGLKPMAVNRVQSIIDDLRNQIISRMVKNDVVNEVAKQ
                     A"
     gene            3138..3419
                     /locus_tag="KLK57_001621"
     CDS             3138..3419
                     /locus_tag="KLK57_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892010.1"
                     /translation="MTESEILRLIRCAGGISQLADEQAAQPDTVTAENYARVVAEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            3794..4909
                     /locus_tag="KLK57_001622"
                     /pseudo
     CDS             3794..4909
                     /locus_tag="KLK57_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            4906..5118
                     /locus_tag="KLK57_001623"
     CDS             4906..5118
                     /locus_tag="KLK57_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892011.1"
                     /translation="MNQNDIEAMIQRYTEAEMAVLDGKSVTFNGQQMTMENLSEIRQG
                     RQEWERRLAALITRRRGHPGYRLARF"
     gene            5046..6626
                     /locus_tag="KLK57_001624"
     CDS             5046..6626
                     /locus_tag="KLK57_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011703754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBE0892012.1"
                     /translation="MGAPPCGSDYTTTGASRVPAGEVLMAILDDVIGVFSPGWKAARL
                     RSRAVIQAYEAVKTTRTHKARRENRTADQLSQYGAVSLREQARYLDNNHDLVIGVFDK
                     LEERVVGKNGIIVEPHPVLRNGAIARDLAAEIRTRWSEWSVSPEVTGQFTRPMLERLM
                     LRTWLRDGEVFAQMVSGRINSLTPSAGVHFWLEALEPDFIPMTSDESNRLNQGVFVDD
                     WGRPEKYLVYKSRPVSGRQMETKEVDAERMLHLKFVRRLHQMRGTSLLSGVLIRLSAL
                     KDYEDYELTAARIAAALGMYIRKGDGQSYEPDGNGSKENERELTIQPGIIYDDLKPGE
                     EIGMVKSDRPNPNLETFRNGQLRAVAAGSRLSFSSTARNYNGTYSAQRQELVESTDGY
                     LILQDWFIGAVTRPMYRAWLKQAVASGVIRLPRDLDRSSLYTAVYSGPVMPWIDPVKE
                     AEAWKIQIRGGAATESDWVRAGGRNPDDVKRRRKAEIDENRKLDLVFDTDPASDKGGS
                     SAATKRQEPQHTDDQSEE"
     gene            6571..8598
                     /locus_tag="KLK57_001625"
     CDS             6571..8598
                     /locus_tag="KLK57_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308856.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HBE0892013.1"
                     /translation="MPQRNDRSRSTPTTSPKNNSWFRMQAGHQSDADIYIYDEIGFWG
                     VTAKQFISDLNALGDITHINLHINSPGGDVFEGIAIFNALKTHGASITVYVDGVAASM
                     ASVIAMVGNPVIMPENTFMMIHKPFGFTGGDAEDMRTYADLLDKVEAVLLPAYAQKTG
                     KTTDEIAAMLADETWMSGAECLAHGFADQVTPAVKAMACIQSKRTEEFKKMPESIRNM
                     ITPPRNSAPRVQDDEPAASRTPVQAAAPVVDENSIRAQVLAEQKARVNGINDLFAMFG
                     GRYQTLQAQCLADPECSLEQAREKLLNEMGRESTPSNKNTPAHIYAGNGNFVGDGIRQ
                     ALMARAGFEKTERDNVYNGMTLREYARMSLTERGIGVSSYNPMQMVGAAFTHSTSDFG
                     NILLDVANKAILQGWEDAPETYEQWTRKGQLSDFKIAHRVGMGGFSALRQVREGAEYK
                     YVTTGDKQATIALATYGELFSITRQAIINDDLNMLTDVPMKLGRAAKSTIADLVYAIL
                     TSNPKISTDNVSLFDKAKHANVLESAAMDVASLDKARQLMRVQKEGERHLNIRPAFVL
                     VPTAMESVANQVIRSSSVKGADINAGIINPVKDFATVIAEPRLDDNSQTTFYLAASKG
                     SDTIEVAYLNGVDTPYIDQMEGFSVDGVTTKVRIDAGVAPVDHRGLVKCTA"
     gene            8685..9008
                     /locus_tag="KLK57_001626"
     CDS             8685..9008
                     /locus_tag="KLK57_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2190 family protein"
                     /protein_id="HBE0892014.1"
                     /translation="MAKNFVEEGKTVAIVASAAISSGDLVQVGDVFAVALTDIPQGET
                     GDGLTEGVFMLPKLKTDDMKTGKKVYLKSGKVQLTNSGSDPLVGVVWADAGTSAEEVP
                     VKLNV"
     gene            9001..9276
                     /locus_tag="KLK57_001627"
     CDS             9001..9276
                     /locus_tag="KLK57_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA breaking-rejoining protein"
                     /protein_id="HBE0892015.1"
                     /translation="MSDPFSRLAARMDAITVRKMGKTASINDVDMTVIPGETLAELNA
                     LSGPAVSLVVFSSGYRPRRGDRVVYDGQQWTVTRHERFNGKPMIFIE"
     gene            9288..9866
                     /locus_tag="KLK57_001628"
     CDS             9288..9866
                     /locus_tag="KLK57_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0892016.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNLPAIKLGTARVRLARR
                     GGKLQYRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKVPLSGPLTQ
                     AFEDARDRIIAAEMPKQLGYALKQQLRLWLTR"
     gene            9863..10264
                     /locus_tag="KLK57_001629"
     CDS             9863..10264
                     /locus_tag="KLK57_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0892017.1"
                     /translation="MNRHTQIRQAVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFIRAQAPDSELDMWMESTIFPALNDIPALSGLID
                     TLIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            10276..11019
                     /locus_tag="KLK57_001630"
     CDS             10276..11019
                     /locus_tag="KLK57_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0892018.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGKGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVS
                     VTGLTVAPASASVKVGATTTLTFTVKPDGASDKAISVHSTDPQTATVTLNGLVATVKG
                     VKQGSVSIVGMTSDGDFVAVAAVAVSAAG"
     gene            11080..11466
                     /locus_tag="KLK57_001631"
     CDS             11080..11466
                     /locus_tag="KLK57_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBE0892019.1"
                     /translation="MFLKQGTFNYEKQSVVLSELSGLQRIEYLAFVQQRTAKFDAEEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASVITTWSYDALGAGAEM
                     VLSLSGMGVIENAGDLEHEVLTPEKS"
     gene            11475..11804
                     /locus_tag="KLK57_001632"
     CDS             11475..11804
                     /locus_tag="KLK57_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBE0892020.1"
                     /translation="MQFVMRLAREFRRADWRRMLSEMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            11776..14841
                     /locus_tag="KLK57_001633"
     CDS             11776..14841
                     /locus_tag="KLK57_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBE0892021.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTTQAASSAATAASVHAQTVEKNAQAHERMAREVEKTRQRMEALSQK
                     MREEQAQAMALAEAQDKAAAAFYRQIDSVKQASAGLQELQRIQQQIRQARNSGGIGQQ
                     DYLALISEVTAKTRVLTQAEEEATRQKVAFIRQLKEQATRQNLSSSELLRAKAAQLGV
                     SSAAEVYIRKMEQAGKATHSLGLKSAAARQEIGVLIGELARGNLGALRGSGITLANRA
                     GWIDTLMSPKGMMLGGVIGGIAAAVYGLGKAWYDGQKEGEEFNRQLSLTGHYAGVTAG
                     QLWTLSRAISGNGITQHAAAGALAQVVGSGAFRGNDIGMVARAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNELHFLTATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     RRTADIDNNLNALGSTLKYLSDLWSRFWDAAMNIGREDSLDEQISALQEKVSRAKRLP
                     WTASSSQVEYDQQRLNELQEKKRQKDLQDAKEQAEQNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINSMQYADQAVRDAAIQRENERYEKALASGKKKTREPRNDEATRLLL
                     QYSQQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLA
                     RKDELIQALTLLDVKQQELQKQTALNELKKKTIQLTSQLAEEERAQRQQHDLDIATVG
                     MGDQQRQRYQVQLSLRQKYQQQLEQLRRDSEQKGTYNTDDYRKAEQALTESLNRQLNE
                     NRRYWQQLEVVQGNWKNGVLRAFQNFTVDADNTAGTAEQVFSSAFSNMGNGLATFVTT
                     GKLNFKSFTSSVLSDMAKILAQATMMKSIKGIGSVLGFDLSSLSLNANGGIYQSADLS
                     RYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADIGGSGMAMFAPQ
                     YNIEINNDGTNGQIGPAALKAVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            14841..15170
                     /locus_tag="KLK57_001634"
     CDS             14841..15170
                     /locus_tag="KLK57_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0892022.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKCDHPSLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            15180..15878
                     /locus_tag="KLK57_001635"
     CDS             15180..15878
                     /locus_tag="KLK57_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBE0892023.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVIWQGRQYQVYPIQGTGFELNGKGSAARPTLTVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            15884..16627
                     /locus_tag="KLK57_001636"
     CDS             15884..16627
                     /locus_tag="KLK57_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0892024.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     DYFRMAPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLSGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRNGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLMCCFGSSVPNHAAIYCGDSELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGIYNDLAAASTFE"
     gene            16564..17172
                     /locus_tag="KLK57_001637"
     CDS             16564..17172
                     /locus_tag="KLK57_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019843089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBE0892025.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGSEAIRALAMQNPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLAGAKSGGVFQVVLGAALIAVA
                     WWNPVGWLGAAAVSGMYAAGASMILGGVAQMLAPKARTPTAASTDNGKQNTYFSSLDN
                     MVAQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            17232..20627
                     /locus_tag="KLK57_001638"
     CDS             17232..20627
                     /locus_tag="KLK57_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBE0892026.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFSIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTFKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPNNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISIGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGEGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFTQ
                     LALGRYMLTVRAVNAWGLQGDPASVSFRIAAPAAPSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLDFFKGQITESHLGKELLEKVELTEDNASKLEEFSKEWKDAS
                     DKWNAMWGVKIEQTKDGKHYVAGIGLSMEDTEEGKLSQFLVAANRIAFIDPSNGNTRP
                     MFVGQGNQIFMNDVFLKRLTAPTITSGGNPPAFSLTPDGRLTAKNADISGNVNANSGT
                     LNNVTINENCRVLGKLSANQIEGDLVKTVGKAFPRDSRAPERWPSGTITVRVYDDQPF
                     DRQIVIPAVAFSGAKHEREHTDIYSSCRLIVRKNGAEIYNRTALDNTLIYSGVIDMPA
                     GHGHMTLEFSVSAWLVNDWYPTASISDLLVVVMKKATAGISIS"
     gene            20695..21294
                     /locus_tag="KLK57_001639"
     CDS             20695..21294
                     /locus_tag="KLK57_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBE0892027.1"
                     /translation="MRKVCAAILSAAICLSVSGAPAWASEHQSTLSAGYLHASTNVPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYAGDKNRQLTRYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGMAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDGRHSNTSLAW
                     GAGVQFNPTESVAIDIAYEGSGSGDWRTDGFIVGVGYKF"
     gene            21359..24322
                     /locus_tag="KLK57_001640"
     CDS             21359..24322
                     /locus_tag="KLK57_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0892028.1"
                     /translation="MAVKISGVLKDGTGKPVQNCTIQLKAKRNSTTVVVNTLASENPD
                     EAGRYSMDVEYGQYSVILLVEGFPPSHAGTITVYEDSRPGTLNDFLGVMTEDDARPEA
                     LRRFELMVEEVARNASAVAQNTAAAKKSASDAGTSAREAATHATDAAGSARAASTSAG
                     QAATSAQSASSSAGTASTKATEASKSAAAAESSKSAAATSAGAAKTSETNAAASQQSA
                     ATSASTATTKASEAATSARDAAASKEAAKSSETNASSSASSAASSATAAGNSAKAAKT
                     SETNARSSETAAGQSASAAAGSKTAAASSASTASTSAGQASASATAAGKSAESAASSA
                     STATTKAGEATEQANAAARSASAAKTSETNAKASETSAESSKTAAASSASSAASSASS
                     ASASKDEATRQASAAKGSATTASTKATEAAGSATAAAQSKNTAESAATRAETAAKRAE
                     DIASAVALEDASTTKKGIVQLSSATNSTSESLAATPKAVKAAYELANGKYTAQDATTA
                     QKGIVQLSNATNSTSEMLAATPKSVKAAYDLANGKYTAQNATTAQKGIVQLSSATNSA
                     SETLAATPKAVKAANDNANGRVPSARKVNGKALSADITLTPKDIGTLNSTTMSFSGGA
                     GWFKLATVTMPQASSVVSITLIGGAGFNVGSPQQAGISELVLRAGNGNPKGITGALWQ
                     RTSTGFTNFAWVNTSGDTYDIYVAIGNYATGVNIQWDYTSNASVTIHTSPAYSANKPE
                     GLTDGTVYSLYTPSEQFYPPGAPIPWPSDTVPSGYALMQGQTFDKSAYPKLAAAYPSG
                     VIPDMRGWTIKGKPASGRAVLSQEQDGIKSHTHSASASSTDLGTKNTSSFDYGTKSTN
                     NTGAHTHSISGTANSAGAHQHKSSGAFGGTNTSIFPNGYTAISNLSAGIMSTTSGSGQ
                     TRNAGKTSSDGAHTHSLSGTAASAGAHAHTVGIGAHTHSVAIGSHGHTITVNAAGNAE
                     NTVKNIAFNYIVRLA"
     gene            24484..24906
                     /locus_tag="KLK57_001641"
     CDS             24484..24906
                     /locus_tag="KLK57_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE0892029.1"
                     /translation="MAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVT
                     WLSPEGEYQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIA
                     TEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPAVPVME"
     gene            25444..>27353
                     /locus_tag="KLK57_001642"
     CDS             25444..>27353
                     /locus_tag="KLK57_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE0892030.1"
                     /translation="MEPISITVATYVATKLIDQFISQEGYGCIKKALFPQKRYVDRLY
                     QLIEETAIEFEETYPVESGAIPFYHSEPLFEMLNEHIFFKEFPDKEILLDKFKEYPSI
                     TPPTQQQLSLFYEMLSLKINNCSKLKKLHIEETYKEKIFDINEELIQVKLILRSIDEK
                     LTFHLSDDWLNEKNSQAIADLGGRYTPELNVKLEIAEIFDGLGRTNDFSKIFYSHIDS
                     FLVAGKKLHSCDVISSELFEINQSLKEISDIYQEINFSKLDEIPINKFNNYVSSCQTA
                     IGGAVSILWELREKSEQVGETKHYSDKYSSTLRMLREFDYACNELRIFINSTTVKLAN
                     NPFLLLEGKAGIGKSHLLADVIKNRITSGYPSLLILGQQLTSDESPWSQIFKRLQLKI
                     TSREFLEKLNLYGKKIGKRVLVFIDAINEGNGNKFWNDNINSFVDEIRCFEWLGLIMS
                     VRTTYRNVTISHENVVRNNFEIHEHIGFQNVELDAVSLFYDYYNIERPSSPNLNPEFK
                     NPLFLKLLCEGIKKNGLTKVPVGFNGISNIFNFLVEGVNKSLASPKKYAFDPSFPLVK
                     DALNEIIKFKLEIGRNSISLKDAHSVVQSVVNDYVADKTFLSALIDEGLLTKGIVRND
                     DNSTEEVVYVAF"
BASE COUNT         6939 a         6272 c         7896 g         6246 t
ORIGIN      
        1 gttatttctg ttttcacctg tacaatattt ttttctccct gtcattagac cagtttgcaa
       61 ttaattgaaa tatataaata aatctgtttt tcacctgcca ggtggagttg cctgtgtcaa
      121 aacgtgttca gatggcggga tatttatgcc ggattttatc cggctttttt gtgtctgaat
      181 ctcattaatc tatttttatg atagaaatat gtttatctac cacttttatc gatcaataat
      241 gtgcacagtt tagtcagtaa gaggaagtta ctgtgagttg tattaatgac ctgaacacgg
      301 gcgatatcag gggtggttcc gttcatctgg atgcgcagac cgttatgcgc cttaagcagt
      361 acaggatcga ccatataaat catcatcctg acaaaccatt accaggtgtg gcgcagattg
      421 tcaggcatgc cgtaaacgcc tggcttaatc agagtggttt tgcatcggag agtgagcaat
      481 gaatcactgg tacaccatta aggcggcgga tgttcgcgga gcggcggata tatctatcta
      541 tgaggagatt ggcggcttcg gtgttactgc aaagcagttc gcggaagacc tgaaagccct
      601 tggcgatgtt tcacatatca atctgaggat ccattcacca ggtggtgatg tgtttgaagg
      661 catcgccatc tataacctgc tacggaatca tccggcagac attacggttt atatcgatgg
      721 tgttgcggct tcaatggctt cggttgtcgc aatggctggc gatcgtgtag tgatgccgga
      781 gaacgccatg atgatgatcc ataaaccgtg gggtatctct ggcggaaatg ctggcgatat
      841 gcgtgattat gctgatttgc tggataaggt ggaaaccgtg ttaatccctg cttatgccag
      901 aaaaacgggc aaatcagcac aggaaattac cgccatgctg gaggatgaaa cctggatgga
      961 tgggaaagaa tgccttaagc acggttttgc tgatgaattg ttgccatccg tcagagcaat
     1021 ggcgcgaatt gaatcgaaac gcacaggaga ttttttacat atgccggaaa ccattaaagg
     1081 aatgattaca ccgccacagg gagcggcaaa tattgctggt aatgaacaga agcgcatcaa
     1141 tggaataagt gaagtgttta gcttgttcgg cagtcgttac gacgggatca aaatggcgtg
     1201 tctggaagat gcatcatgta caccggaaat ggcccgtgaa aagctgttga acgagctggg
     1261 gcgcgagtcc acgccatcca ataaaaatac cccgcctcat atctatgccg gaaacggaaa
     1321 cataacaggt gatgcaattc gtcaggggct ttattcccgt cttgggtatg aacgccctga
     1381 acgaggcaac ccttacgcga tgatgagcct ttttgaaatg gcccaggcat cactggttga
     1441 tcgtggtatc actgtgagcg gttttattaa tcgctcgcag gttgttaatg cggcttttac
     1501 acacagcagc agcgattttt ctcatattct ggctggtggc gctgaaaaat cagtactgaa
     1561 aggctggcag gacagcggcg aaacgttcca gaaatggacg cgtaccggtt cgctttcaaa
     1621 ctttcatgaa gcaaagcgcg ttggtctgaa tggtttttca aagctggata aagtaccgga
     1681 aggcgcggaa tataaataca tcaccactag cgataaaggt gtacctattg cgctggccac
     1741 gtacgggaat attttttccg ttacccgtca ggccattatc aacgatgacc tgacccagtt
     1801 aactacaatc cccatggcga tgggacgcgc agctgccaga acagttggca atctggttta
     1861 tctcctgtta accagcaacg gcaagtttac ggatggtaaa gcgttattcc atgccgatca
     1921 taaaaatctt attgcgaagg atatggacat ggaggggctt aacgaagccc gtaagctgat
     1981 gcgcctccag gaagacgcta acggcgattc gctgaatatt acccccgcat ttgtcctggt
     2041 ccccgccgcg ctggagtctg ccgcacatcg cgccattctg tcatcgtcat cactctttcc
     2101 ggttgatggc gtgggcacta tcaatcagaa ccccggcatc attaacgtgg tgaaagatat
     2161 ggcggaggta atcgttgagc cacgtcttga taaagccaat aacaaggagt ggtatgtggc
     2221 agcggcgaag ggtatggaca cgatagaggt cgcttatctt gatggtattg atacgccata
     2281 tcttgaggag caggagggct ttactgttga tggcgtcgcc tggaaggtgc gcatagatgc
     2341 aggtgtcgcg gccctcgact atcgcggatt actgaaatcg agtggagcat gacaacaagg
     2401 gcggcgatag ccgccttttt ttacgggtcc tcccggtggg gtggtctgcc acggggcggg
     2461 agcggcgcgg aaaaaggcta gtttttgcat tttcattcgt catcatcatc tttctatgat
     2521 attgaatttt aagtgttttt atttttggta tgttaatttt gcttgtttta tgctcaacat
     2581 atagcgcatt ttttgacctc ttctgaaagt tgttcgcaag atgcatgttt aaaacattct
     2641 ggagcgggta tggatcgaga actgaaaaat ctgatgctga acattaatca actggcggcg
     2701 atagcgggaa tatgtcgtca gactgcggcg gcaaggctga aaaacatcca gccagccgga
     2761 gggcatgaca agctaaaact ctatcgggtg actgacattc tgacctgttt tcttgatctt
     2821 cccgttccgg catcactgga agaaatggag ccacatgacc gtaaggcctg gtatcagtcc
     2881 gaacgtgagc gcctcaagtt cgaacaggaa acggcgcaac tcatacccgc cgatgatgtg
     2941 cgaaaagaga tggctatatg gggggaaatc gtaagcgagg aactggcaaa actccccaat
     3001 attctggcac gtgatgccgg gcttaaaccg atggcagtaa acagagtgca gtcaattatt
     3061 gacgatttgc gtaatcagat tatcagccgg atggtaaaaa atgacgtagt gaatgaggtc
     3121 gcaaaacagg catgataatg accgaatctg aaatactgcg attaatccgc tgtgctggtg
     3181 gaatcagcca gttggctgac gaacaggccg cgcagccgga tacagtcacc gctgaaaatt
     3241 acgcgcgtgt ggtggctgag gtgatgcgcc gtgacggtat tgagcttaac ggcgttgata
     3301 tgcgcaacat acgaaccaga gttcttgagt tgctggcata ccgtcgccgt tctcaacaac
     3361 ggagggagag cgcgaaaaat acttaccagt ggaggaagcc ggagcactta cggcggtaac
     3421 tggttgatat tatcgaaagt gcaaaaatgc acttagcaac tggtggcgag ttgcagatct
     3481 gcaactcgac tatgaaacta cggaaactac ccgtagtttg ggtagtaaga gtaacaccca
     3541 gattttgggg cttactcgcg atacccaaat aaagggtatc ggtggaagaa atatcgtttc
     3601 tcatatgtga gttccgagag cggaattccg cctctgattt gtcattgtga tcatgcatag
     3661 cggcaataat taatattgct tcctttttgg cttttttatc gcttaagcag gtactaaatc
     3721 tgggactatg ttgtaggggg cataactcaa tagtacctta taatcattta gttacctttt
     3781 atttacttct tcctggatgg agggtccggc agctgcttac cagacactct cgcaactcgt
     3841 ttacaaactg cttactgcag aacaggaata cgagacaacc ggaagtgaag aaacactcaa
     3901 gacggttatc aataccgact ggggattacc ttatcttccc cgtgccagca tggagcaacg
     3961 aaaaagtgaa ctgcttgagc agcgggcaga gccagttcct tcccgcagtg tgccggatgg
     4021 cgttaatttc ctggtggcga cagttgatgt gcaggcggga cgtcatcgcc gttttgtggt
     4081 tcaggtaacg ggctatggca gccgtggcga acgctggatt attgatcgtt acaacatcac
     4141 gcagtcattg cgcggtgaca gcgacgggga gagccagcga attgatccgg ccagctatcc
     4201 ggaagactgg gatgtcctgc tgacggatgt ttttcataaa agctggccgc tggcctccga
     4261 tccttctcaa caaatgcgac tgatggcaat ggcggtggac tccggcggtg aagacggggt
     4321 cactgataat gcctataaat tctggcgtcg ttgccgtcgt gatggccttg gtaaacgtat
     4381 ttacctgttt aagggcgaca gcatccggcg cgcaaaactg atcacccgta cattccctga
     4441 taacaccgga cgaacgggcc gacgggcgca ggccgcaggt gatgtgccgc tctggcttct
     4501 tcagacggat gccctgaaag accgggtgaa taacgcgtta tggcgtgact cgccaggtcc
     4561 cggctatgtg catttccctg actggctggg gagctggttt tacgacgaac tgacgtatga
     4621 agagcggagt agtgacggga aatggagtaa gccgggtcgc ggtgccaacg aagcttttga
     4681 cctgatggtg tatgccgagg ctctggtcat tctgcatgga tacgaaaaga tccgctggcc
     4741 ggatgcaccg gagtgggcga gccgggaaac ctggctggag tgtgtcccgg acagtaccga
     4801 accgtcaccc tcaccggaac cggtatccac gcctgttaaa aaacaaaaac ggaagaaaac
     4861 agtaactgac gatgttaacc cctggctgac ttccggagga tggttatgaa ccagaatgat
     4921 atcgaagcca tgattcagcg ttatacggaa gctgaaatgg cggtgctgga cggaaaatcc
     4981 gtcaccttta atggtcagca gatgaccatg gaaaacttat ctgagatccg gcagggacgg
     5041 caggagtggg agcgccgcct tgcggctctg attacacgac gacgggggca tcccgggtac
     5101 cggctggcga ggttctgatg gctattcttg atgatgtgat tggcgttttt tcaccaggat
     5161 ggaaagcggc aaggctgcgt tcccgtgcgg tgatccaggc ttatgaggcc gtaaaaacga
     5221 cgcggacaca caaagcccgg cgggaaaacc gaactgccga ccagttaagc cagtacgggg
     5281 ccgtgtcgtt acgtgagcag gcccgctacc ttgataacaa ccacgatctg gttattggtg
     5341 tatttgacaa gctggaagaa cgggtggtgg ggaaaaacgg gattattgtc gagccacatc
     5401 cggtattacg caatggggcc attgcccgtg atctggcagc ggagattcgc acccgatgga
     5461 gtgaatggtc tgtcagcccg gaagtcaccg ggcagtttac ccgtccgatg ctggaacgtc
     5521 tgatgctgcg tacctggctg cgcgatggtg aggtgtttgc ccagatggtt tccgggcgca
     5581 taaacagcct gacgccttct gccggtgttc atttctggct ggaggcgctc gagccagact
     5641 ttattcccat gaccagtgat gagagcaaca ggctgaatca gggcgtgttt gttgatgact
     5701 gggggcgtcc cgaaaaatat ctggtgtata aaagccgtcc cgtatccgga cggcagatgg
     5761 aaaccaaaga agtggatgca gagcgaatgc tgcatcttaa atttgttcgc cgtctgcacc
     5821 agatgcgcgg gacgtctttg ttgtccggtg tgctgatccg cctcagtgcc ctgaaagatt
     5881 atgaagatta tgagctgact gcagcaagga tcgccgctgc tctggggatg tacatccgga
     5941 aaggcgacgg gcagagctat gaaccggatg gtaatggcag caaggagaat gaacgcgagc
     6001 ttaccattca gccaggcatt atttacgacg atctgaaacc cggcgaagaa atcggaatgg
     6061 tgaagtcgga tcgtcccaat cctaaccttg aaacttttcg taatggtcag ttgcgtgccg
     6121 tggcggcggg cagtcgtctg agtttttcca gtacggcacg caactataac ggcacttaca
     6181 gcgcccagcg tcaggaactg gttgagtcta ctgatggcta cctgatcctg caggactggt
     6241 ttattggtgc cgtcacccgc ccgatgtatc gtgcctggct gaaacaggct gtggcatccg
     6301 gtgttatcag gctaccccgc gatcttgacc gttcttcact gtataccgcg gtgtattccg
     6361 gaccggtgat gccgtggatt gaccctgtta aggaggctga ggcctggaaa atccagattc
     6421 gtggtggagc ggcgacagaa tcagactggg tacgtgctgg tggtcgtaat ccggatgatg
     6481 tcaaacgtcg gcgcaaggcc gaaattgatg aaaaccgcaa gctggatctg gtatttgata
     6541 ccgatccggc cagtgataaa ggaggcagca gtgccgcaac gaaacgacag gagccgcagc
     6601 acaccgacga ccagtccgaa gaataattcc tggttcagga tgcaggctgg tcaccagagt
     6661 gacgcggata tttatattta tgacgagatt ggtttctggg gtgttacagc gaagcagttt
     6721 atcagtgatc tgaatgcact gggcgatatc acccacatta atctccatat taattcaccg
     6781 ggtggcgatg tctttgaagg catcgccatt tttaatgcgc ttaaaacaca tggtgcgtcc
     6841 attaccgttt atgtcgacgg tgtggcggcg tcaatggcgt cggtcattgc gatggtggga
     6901 aacccggtca ttatgccgga aaacaccttc atgatgattc ataaaccatt tggctttacg
     6961 ggcggtgatg cggaggacat gcgcacctat gccgacctgc tcgataaggt tgaggcggtt
     7021 ctgttacccg cttatgcaca gaaaaccggg aaaaccaccg atgaaattgc tgccatgctg
     7081 gcggatgaga cctggatgtc cggtgccgaa tgtctggcac atggatttgc tgatcaggta
     7141 acgccagccg ttaaggcaat ggcatgtatt cagtcaaaac gtacagagga atttaaaaag
     7201 atgccggaat ccattcgaaa catgattact ccgccacgca acagtgctcc acgcgtacag
     7261 gatgatgaac ctgcagcctc ccggacgcca gtgcaggcag cagcacccgt ggtggatgaa
     7321 aacagtatcc gtgcgcaggt actggcagag caaaaagcgc gtgtaaacgg tattaatgat
     7381 ctgtttgcca tgtttggcgg gcgttatcag acactgcagg ctcagtgtct tgccgatcct
     7441 gaatgttcgc tggagcaggc ccgcgaaaag ctgttgaacg agatggggcg cgagtccacg
     7501 ccatccaata aaaatacccc ggctcatatt tatgccggta acggtaattt tgtgggggac
     7561 gggatccgcc aggcgctgat ggcgcgtgcc ggatttgaaa aaaccgaacg tgataatgtc
     7621 tacaacggga tgaccctgcg tgaatatgcc cgtatgtcac tgactgaacg gggtattggg
     7681 gtttccagtt ataacccgat gcagatggtc ggtgcggcgt tcacacacag tacgtctgac
     7741 ttcggtaata ttctgctgga tgttgcgaac aaagccattt tgcagggctg ggaagatgcc
     7801 cctgaaacct atgaacagtg gacgcggaaa ggtcagttgt ctgattttaa aattgcccat
     7861 cgtgtgggta tggggggctt cagtgctctg cgtcaggtgc gtgaaggggc ggaatataaa
     7921 tacgtcacca ccggagataa acaggccact attgcactgg cgacctatgg cgagctgttc
     7981 agtatcaccc gtcaggccat tatcaatgat gatctgaata tgctgaccga tgtcccgatg
     8041 aaactgggcc gtgcggcgaa atccactatt gccgatctgg tttatgccat tctgacgtct
     8101 aacccgaaaa tctccacaga taatgtaagt ctgttcgata aagcgaaaca tgcaaacgta
     8161 ctggagagcg ctgcaatgga cgtggcatcg ctggataaag cccgccagtt gatgcgcgtt
     8221 cagaaagagg gggagcgtca tctgaatatt cgtcctgcgt tcgtactggt accgacggcg
     8281 atggagtctg ttgctaacca ggtcattcgc tcctcaagtg tcaagggggc tgacattaac
     8341 gccggtatta ttaacccggt gaaagatttt gcgaccgtta ttgcagagcc tcgtcttgat
     8401 gataacagcc agaccacttt ctacctggct gcgtccaaag gctccgatac gattgaagtg
     8461 gcttatctca acggtgtgga tacgccatat attgatcaga tggagggctt cagtgtggat
     8521 ggcgtgacaa cgaaagtgcg tattgacgcc ggtgtcgcgc cagttgatca ccgcggtctg
     8581 gtgaaatgta cggcgtaaac gtcgcagaca acaactctga tggcccgtaa gggctttttt
     8641 tgtacctgaa atcagcccct gaacggggct gtgcggagac agttatggca aagaattttg
     8701 tagaagaagg aaaaacggtg gcgattgttg ccagtgcagc catcagcagc ggagatctgg
     8761 tgcaggtggg cgatgttttt gcggtggcgc tgaccgatat tccacagggt gaaacaggcg
     8821 acggcctgac cgaaggtgtg tttatgctgc ctaagctgaa aacggatgac atgaaaacgg
     8881 gtaagaaggt ttatctgaag tccggaaaag ttcagctgac taacagcggc tctgatccgc
     8941 tggtcggggt tgtctgggca gatgccggaa ccagtgcaga agaagtgccg gtaaaactca
     9001 atgtctgatc ccttttcccg gttggcagcg cgtatggatg ctatcacggt cagaaagatg
     9061 ggaaagacag cctcgattaa tgatgtcgat atgactgtga tcccgggaga aacactggca
     9121 gagctgaatg ctctgtccgg gcctgcggtc tctctggtgg tgttttcttc gggataccgc
     9181 ccacggcgcg gggatcgcgt tgtttatgac ggacaacaat ggacggtcac acggcatgaa
     9241 cgctttaacg gtaagccaat gatctttatt gagtaaagag gtgtgggatg aaggggcttg
     9301 agaatgccat ccgtaatctg aacagccttg atacccgtat ggtgccacag gccagcgcat
     9361 gggcgataaa ccgtgtggca cagaaagcgg tctcggttgc cacccggcag gttgccggga
     9421 ataccgttgc gggagataac caggtgaaag ggatccccct gaaactggta cgtcagcgtg
     9481 tccgggtgtt taaagccagt ccgtcaggaa aaatgacggc caggatccgc gttaaccggg
     9541 gcaatctgcc cgccattaag ctggggacag cccgggtcag actggcccgg cgtggtggaa
     9601 aactgcagta ccgtggcagt gtgctgaagg tgggtaaata tcttttccgg gatgcgttta
     9661 ttcagcaact ggcgaatggt cgctggcatg tgatgcggcg tattgatggc aaaaatcgtt
     9721 accccattga tgtggtgaaa gtcccgctgt ccggaccgct gacacaggca tttgaagatg
     9781 cccgcgaccg cattattgct gcggaaatgc cgaaacagct ggggtatgca ctgaaacaac
     9841 aactgaggtt atggctgacc cgatgaaccg acatacacaa atccgccagg ccgtactggc
     9901 acgccttcgg gaacagtgtg gagacagcgc cacgtttttt gacgggcttc cggcatttat
     9961 tgatgcgcag gaactgcctg ccgtggcggt gtggctgagt gatgctcagt acaccggaaa
    10021 aatgacggat gaagatgact ggcaggctgt tctgcatatt gctgtcttca tccgggcaca
    10081 ggcaccggat tcagagctgg atatgtggat ggagagcacc attttcccgg ccctgaatga
    10141 tataccggca ctttccggac tcatcgacac cctgatccct ctcggtttta actatcaacg
    10201 tgataatgag atggccacct gggcgatggc ggaaatcacg taccagatca cgtacacgaa
    10261 ttaaaggagg tggcaatgac cacaccaaat ccactggcaa aaacgaaagg tgcgggaacg
    10321 acgttctgga tgtacaccgg caagggcgat gcgtttgcga accctttatc ggacactgac
    10381 tggctgcgtc ttgcgatggt gaaggatctg caacctggcg aaatgaccgc tgatgcagaa
    10441 gatgacactt atctcgatga tgaagatgca gactggaaaa cgacaaccca ggggcagaaa
    10501 tccgtcggtg atacttcggc gacgctggcc tggcgtccgg gtgacagcgg gcagaaaaaa
    10561 ctggttcagt tgttcgactc cggtgaagtc tgcgcgtttc gtatcaaata tcccaacggc
    10621 actgttgatg ttttccgtgg ctggctgagc tcactgggta aaaccattgc ctcaaaagac
    10681 gtgatgaccc gcacagtgaa aatcagcggt gtggggcgtc cgtatctggc agaggaaggc
    10741 actgaaacag tgagcgttac cgggctgacg gtggcaccgg catctgccag tgtaaaagtg
    10801 ggagcaacca ccacgctgac ctttacagta aaacctgacg gagccagtga caaagcgatc
    10861 agtgtgcatt cgacagatcc acagactgcc acggtgaccc tgaacgggct tgtggccacg
    10921 gtgaaaggcg tgaagcaggg cagtgtcagc attgtgggca tgacttctga cggcgatttt
    10981 gtggcagtgg ctgcggtggc tgtcagcgcc gcaggttaac aggacgatac tcatcatttg
    11041 ccccggttat ccggggcttt tttgcaggtg gagaacatga tgtttctgaa acagggcacg
    11101 tttaattatg aaaagcagtc cgtggtgctc agtgagctgt ccgggctgca gagaattgaa
    11161 tatctggcgt ttgttcagca gcgaacggca aagtttgatg ccgaagaggg agaactgccg
    11221 gaggctgaac gacagattgc ttttctgcgg atggggatgg atatcaatgc ctggctggtt
    11281 tcccgctcac tgtggaatgc ggaacagtct caggatgttg agacgctttg cgcatccgtt
    11341 attacaacat ggtcgtatga tgccctggga gcgggggcgg agatggttct gtcgctgagc
    11401 ggtatgggag tcattgagaa tgccggggat ttggagcatg aggtgctgac gccggaaaag
    11461 tcctgacgcg ggaaatgcag tttgtcatgc ggcttgcccg ggagttccgg cgggcagact
    11521 ggcggcggat gctgtcggaa atgtcggcca ctgagcttgg tgagtggggc gattatttcc
    11581 ggatgcagag cttcagtgat gtgtggatgg atgcgcagtt tgcctcgctg aaggcattga
    11641 tcgtgagaat ggtgtccggt agcagtgatg ctgcggtggc tgatttcagc cttttaccgg
    11701 aagagaacgg gataccggag cgaacggacg aagaactgat gcatcttggg gaaggtattt
    11761 ccggaggtgt gcgttatgga ccagatagcc aacctggtca ttgatttggg gattgatgcg
    11821 gcagagttta aaaatgaaat tccccgtatc aaaaaccttc tgaatggtgc agccagcgat
    11881 gcagaacggt cttctgcccg tatgcagcgt tttatggagc gtcagactca ggccgcccgg
    11941 cagacaacgc aggcagcttc ttcggctgca acagccgcat ccgtccatgc gcagacggtg
    12001 gagaagaacg cacaggctca tgaacgcatg gcccgcgagg tggagaaaac ccgccagcgc
    12061 atggaggcac tgagccagaa aatgcgcgag gaacaggcgc aggccatggc tctggcggag
    12121 gctcaggata aagcggctgc tgcgttttat cgtcagattg acagtgtgaa acaggccagt
    12181 gcggggctgc aggaattaca gcgtattcag cagcagatcc gacaggccag aaacagtggc
    12241 gggattggtc agcaggatta tctggcgctg atttctgagg ttactgcgaa aacccgtgtt
    12301 cttacacagg ctgaggaaga ggctacccga cagaaagtgg cgtttatccg tcagcttaaa
    12361 gagcaggcaa cccgccagaa tctttcttct tctgagttgc ttcgtgctaa ggctgcccag
    12421 ctgggggtaa gcagtgctgc agaagtgtat atccgcaaaa tggagcaggc aggaaaagcc
    12481 acgcattcgc tgggtctgaa aagtgcagcg gcccgccagg agataggcgt tctgataggt
    12541 gaactggctc gcggcaattt aggtgcgctg aggggatccg ggataacgct ggctaaccgt
    12601 gccggatgga tagacacact gatgtcaccg aaaggcatga tgctgggcgg ggttattggc
    12661 ggtattgccg cggccgtcta tggtctgggt aaagcctggt atgatggtca gaaggagggg
    12721 gaagaattta accgccagtt gtcgctgacg gggcattatg ccggagtcac tgccgggcag
    12781 ctgtggacgc tcagtcgtgc tatttccggg aatggtatca cgcaacatgc tgcagccggt
    12841 gcgctggctc aggtggtggg gagtggtgca tttcgtggaa acgatatcgg tatggtggcg
    12901 agagctgccg cacagatgga gcgatcggtt ggccagtcgg tcagcgatac cataaatcag
    12961 tttaagcggc tgaaggatga tcctgtaaat gccgcgaagg ctctggacaa tgagctgcat
    13021 tttcttactg ccactcagct tgagcagata cgcgtccttg gggaacaggg gcggtccagt
    13081 gatgcggcac ggatagccat gtctgcactg gcagaggaaa ccggtcggcg tactgcggat
    13141 attgataata acctcaatgc gctgggcagt acgctgaagt atctgtctga tttatggagt
    13201 cgtttctggg atgcggccat gaatattggt cgtgaagact cgctggatga acagatttcc
    13261 gctttacagg agaaagtgtc gcgggcgaaa agactcccct ggacggcatc atcttctcag
    13321 gttgagtacg atcagcagcg tcttaacgag cttcaggaga aaaaacgcca gaaggatttg
    13381 caggatgcaa aagagcaggc agagcagaat tatcaggagc aacagaaacg ccgtaatgct
    13441 gaaaatgctg cactgaaccg gatgaatgaa acggaagcag cacgacatca gcgtgaaatt
    13501 gcgcgtatta attccatgca gtacgccgat caggctgtca gggatgcggc gatacaacgt
    13561 gaaaatgaac gttacgagaa agccctggca tccggtaaga aaaaaacacg cgaaccccgt
    13621 aatgatgagg ccacccggtt attgctgcag tacagtcagc aacaggcaca ggtggaagga
    13681 cagattgctg ctgccagaca gtcagcaggc attgccacgg aaaggatgac agaagcgcat
    13741 aaacagcttc tggctctgca gcagcgcatc agcgacctgg acgggaaaaa actgacggca
    13801 gatgaaaaga gtgtgctggc ccgtaaagat gaactgattc aggcactgac gctgctggat
    13861 gtaaaacagc aggagcttca gaaacagacg gcactcaacg agctgaagaa aaaaacaatt
    13921 cagctgacca gtcaactggc tgaagaagag cgcgctcagc gtcagcaaca tgacctggat
    13981 atcgccacgg tgggtatggg tgatcagcag cggcagcgat atcaggtaca actgagtctt
    14041 cgccagaaat accagcaaca gctggagcag ttgaggcggg atagtgagca gaaaggaaca
    14101 tataacacgg atgactacag aaaggccgag caggcgctga cggagagcct gaaccgacaa
    14161 ctgaatgaga atcgccgtta ctggcaacag cttgaagttg tgcagggtaa ctggaaaaac
    14221 ggagtcctgc gtgcatttca gaattttacc gtggatgcag ataatacggc aggaacagca
    14281 gaacaggtgt tctcgtcagc cttcagcaac atgggaaatg gcctggcaac ttttgtcact
    14341 accggcaaac tcaatttcaa atccttcacc tcttctgtgc tgtcagatat ggcgaaaatc
    14401 ctggcgcagg caaccatgat gaaatcgata aaagggattg gcagtgtact gggatttgat
    14461 ctcagcagcc tttccctgaa tgccaatggg gggatttatc agtctgctga tttgagtcgt
    14521 tacagtggca cggtggttaa ccgtccgacg ttttttgctt ttgcaaaagg cgcgggtgtg
    14581 atgggggaag cgggacctga agccattctg ccactgcgtc gtggtgctga cggtaagctg
    14641 ggggttgtgg cggatattgg tggttcaggt atggcgatgt ttgccccgca gtacaacatc
    14701 gagatcaata acgatggcac gaacgggcag ataggtccgg ctgccctgaa ggcggtttat
    14761 gacctcggga aaaaagcggc agcggacttt atgcaacagc aggcccgtga tggtggtcgg
    14821 ttaagtggag catatcggta atggagacgt ttcactggaa agtgcgcccg gatatgaatg
    14881 tggtatcaga gccgaaagtg gtgacagtga agctgggcga tggttatgaa cagcgtcgtg
    14941 cggcgggact gaataaccag ttgtcgactt acagcgtgac gatacgtgtt cgtaaatgtg
    15001 accacccatc tttaaaagcc tttctggaac ggcacggtgg cgtccgtgca tttcagtgga
    15061 cgccacctta tgactggaaa ccgatcaggg tggtttgtcg taaatggtcg gcaagcgtgg
    15121 gggcgctgtg ggtaaccata acggcagatt ttgaacaggt cgtggcatag gaggctctga
    15181 tgcaggatat tccacaggaa acacatcatg agacgacacg cctcactcag tcagcccagg
    15241 tggtgctctg ggaaatcgat ctgacagagg tcggtggtga acgttatttt ttctgtaatg
    15301 agcagaacga aaaaggtgag ccggttatct ggcaggggcg gcagtatcag gtatacccca
    15361 ttcaggggac gggatttgaa ctgaacggca agggcagtgc tgcccgtccg acactgacgg
    15421 tttctaacct gtacggtatg gtcaccggga tggcggaaga tctgcagagt ctggtcggcg
    15481 gaacggtggt ccggcgtaag gtttacgccc gttttctgga tgcggtgaac ttcgtcaacg
    15541 gaaacagtga cgccgatccg gagcaggagg tgatcagccg ctggcgcatt gagcagtgca
    15601 gcgaactgag cgcggtgagt gcctcttttg tactgtccac gccgacggaa acggacggcg
    15661 ctgtttttcc gggacgtatc atgctggcca acacctgcac ctggacctat cgcggtgatg
    15721 agtgcggtta tcacggtccg gcagtcgcgg atgaatatga ccagccgacg tccgatatca
    15781 cgaaggataa atgcagcaaa tgcctgagcg gctgtaagtt tcgcaataac gtcggcaact
    15841 ttggcggctt cctttccatt aacaaacttt cgcagtgaat cccatgacag agacagaatc
    15901 agcgattctg gcgcacgccc ggcgatgtgc gccagcggag tcgtgcggct tcgtggtgag
    15961 aacgccggag ggggaaagat attttccctg cgtgaatatc tccggtgagc cggaggatta
    16021 tttccggatg gctccggagg actggctgca ggcagaaatg cagggtgaga ttgtggcgct
    16081 ggtccacagc caccccggtg gtctgccctg gctgagtgag gccgaccggc ggctgcaggt
    16141 gcagagtgat ttgccgtggt ggctggtttg ccggggggcg attcacaagt tccgctgtgt
    16201 gccacatctt tccgggcggc gctttgagca cggggtgacg gactgttaca cgctgttccg
    16261 ggatgcttac catctggcgg ggattgagat gccggatttt catcgcgagg atgactggtg
    16321 gcgtaacggt cagaatctct atctggataa tctggaggcc acagggctgt atcaggtgcc
    16381 gttgtcagcg gcgcagccgg gcgatgtgct gatgtgctgt tttggttcat cggtgccgaa
    16441 tcatgccgcc atttactgtg gtgacagcga gctgctgcac catattcctg aacaactgag
    16501 caaacgagag aggtacaccg acaaatggca gcgacgcaca cactccctct ggcgtcaccg
    16561 ggcatggcac gcatctgcct ttacggggat ttacaacgat ttggccgccg catcgacctt
    16621 cgagtgaaaa cggggtccga agccatccgg gcgctggcca tgcagaaccc ggcgtttcgt
    16681 cagaaactga gcgacggctg gtaccaggta cgcattgccg ggcgtgatgc aggtgaaacc
    16741 gaattgtctg cccgtcttaa tgagccgctg gcaaatggtg ccgtgatcca catcgtgccg
    16801 cgtctggcgg gagctaaaag tggcggtgtg tttcaggtgg tgctgggggc ggcgctgatt
    16861 gcggtggcat ggtggaaccc tgtgggctgg ctgggtgccg cggctgtatc gggcatgtat
    16921 gcggcagggg ccagtatgat cctgggcgga gtggcgcaga tgctggcacc gaaagccagg
    16981 acgcccacgg cagcaagtac agataacggc aaacagaaca cctatttctc ctcactggat
    17041 aacatggttg cccagggcaa tgttctgccc gttctgtacg gtgaaatgcg cgtggggtcg
    17101 cgggtggtct ctcaggagat cagcacggca gacgaagggg atggtggtca ggttgtggtg
    17161 attggtcgct gatgaaaaac gtttatgtga aaccgcctcc gggcggtttt gtcgtttatg
    17221 gagcatgacg aatgggtaaa ggcagcagta aggggcatac tccgcgcgaa gcgaaggaca
    17281 acctgaagtc cacgcagttg ctgagtgtga tcgatgccat cagcgaaggg ccggttgaag
    17341 gtccggtgga tggattaaaa agcgtgctgc tgaacagtac gccagtgctg gacagtgagg
    17401 ggaataccaa tatatccggc gtcacggtgg tgttccgggc cggtgagcag gagcagacac
    17461 cgccggaggg atttgaatcc tccggctccg agacggtgct cggtacagaa gtgaaatatg
    17521 acacgccgat cacccggacc atcacgtcgg caaacattga ccgtctgcgt tttactttcg
    17581 gcgtgcaggc actggtggaa accacctcaa agggggacag gaatccatcg gaagtccgcc
    17641 tgctggttca gatacaacgt aacggtggct gggtgacgga aaaagacatc accattaagg
    17701 gtaaaaccac ttcacagtat ctggcctcgg tggtggtgga taacctgccg ccgcgcccgt
    17761 tcagtatccg gatgcgcagg atgacgccgg acagcaccac agaccagctg cagaacaaaa
    17821 cgctctggtc gtcatacacc gaaattatcg atgtgaaaca gtgctacccg aacacggcac
    17881 tggtcggcgt gcaggtggac tcggagcagt tcggcagcca gcaggtgagc cgtaattatc
    17941 atctgcgcgg gcgtattctg caggtgccgt cgaattataa cccgcagacg cggcaataca
    18001 gcggtatctg ggacggaacg tttaaaccgg catacagcaa caacatggcc tggtgtctgt
    18061 gggatatgct gacccatccg cgctacggca tggggaaacg tcttggtgcg gcggatgtgg
    18121 ataaatgggc gttgtatgtt atcggccagt actgcgacca gtcagtgccg gacggttttg
    18181 gcggcacgga gccgcgcatc acctgtaatg cctggctgac cacacagcgc aaggcgtggg
    18241 atgtgctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac gggcagacgc
    18301 tgacgttcgt gcaggaccga ccatcagata aggtgtggac ctataaccgc agtaatgtgg
    18361 tgatgccgga tgatggcgcg ccgttccgct acagcttcag cgccctgaag gaccgccata
    18421 atgccgttga ggtgaactgg attgacccga acaacggctg ggagacggcg acagagcttg
    18481 ttgaagatac gcaggccatt gcccgttacg gtcgtaatgt tacgaagatg gatgcctttg
    18541 gctgtaccag ccgggggcag gcacaccgcg ccgggctgtg gctgattaaa acggagctgc
    18601 tggagacgca gaccgtggat ttcagcgtgg gcgcagaagg gcttcgccat gtgccgggcg
    18661 atgttattga aatctgcgat gatgactatg ccggtatcag catcggtggt cgcgtgctgg
    18721 cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg ccatcctccg
    18781 gcaccacgct gataagcctg gttgacggtg agggtaatcc ggtcagcgtg gaggtccagt
    18841 ccgtcaccga cggcgtgaag gtgaaagtga gccgtgttcc tgacggcgtt gctgaataca
    18901 gcgtgtgggg gctgaagttg ccgacgttgc gccagcgcct gttccgctgc gtgagtatcc
    18961 gtgagaacga cgacggcacg tatgccatca ctgccgtgca gcatgtaccg gagaaagagg
    19021 ccatcgtgga taacggggcg cactttgacg gcgaccagag cggcacggtg aatggtgtca
    19081 cgccgccagc ggtgcagcac ctgactgccg aagtcaccgc agacagcggg gaatatcagg
    19141 tgctggcccg ctgggacacg ccgaaagtgg tgaagggcgt gagcttcctg cttcgcctga
    19201 ccgtggcagc ggatgacggc agtgagcggc tggtcagcac ggcccggacg acggaaacca
    19261 cataccgctt cacgcaactg gcgctggggc gttacatgct gacggtccgg gcggtaaatg
    19321 cgtgggggct gcagggcgat ccggcatcgg tatcgttccg gattgccgca ccggcagcgc
    19381 cgtctcggat tgagctgaca ccgggctatt ttcagataac cgccacgcca catcttgcgg
    19441 tttatgaccc gacggtacag tttgagttct ggttctcgga aaaacggatt gcggatatca
    19501 ggcaggttga aaccacagcc cgctatcttg gcacggcgct gtactggata gctgccagta
    19561 tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac accgttggca
    19621 aatcggcatt cgtggaggct gttggtcggg cgagcgatga tgcggaaggt tacctggatt
    19681 ttttcaaagg ccagataacc gaatcccatc ttggcaagga gctgctggaa aaagtcgagc
    19741 tgacggagga taacgccagc aaactggagg agttttcgaa agagtggaag gacgccagtg
    19801 ataagtggaa tgccatgtgg ggcgtcaaaa ttgagcagac caaagacggc aaacattatg
    19861 tcgcgggtat tggcctcagc atggaggaca cggaggaagg caaactgagc cagttcctgg
    19921 ttgccgctaa ccgtatcgcg tttattgatc cgtctaatgg caacacaaga ccaatgtttg
    19981 ttggtcaggg caatcagata ttcatgaacg acgtgttcct gaaacgcctg acggctccga
    20041 ccattaccag cggcggtaat cctccggcat tttccctgac accggacggg cggctgacgg
    20101 cgaaaaatgc cgatatcagc ggtaacgtga atgcgaactc cgggacgctc aacaacgtca
    20161 cgattaacga gaactgtcgg gttctgggaa aactgtccgc gaaccagatt gaaggcgatc
    20221 tcgttaaaac agtgggcaaa gctttccccc gggactcccg tgcaccggaa cggtggccat
    20281 cagggaccat taccgtcagg gtttatgacg atcagccgtt tgaccggcag attgttattc
    20341 ctgcggtggc attcagcggc gctaaacatg agagagagca tactgatatt tactcctcat
    20401 gccgtctgat agtgcggaaa aacggtgctg aaatttataa ccgtaccgcg ctggataata
    20461 cgctgattta cagtggcgtt attgatatgc ctgccggtca cggtcacatg acgctggagt
    20521 tttcggtgtc agcatggctg gtaaatgact ggtatcccac agcaagtatc agcgatttgc
    20581 tggttgtggt gatgaagaaa gccaccgcag gcatcagtat cagctgaatt ttataaccca
    20641 tatacgggcg ccagaaatgg cgcctttttt attgcagaaa agcgagaggt aattatgcgt
    20701 aaagtttgtg cagccatttt gtccgcagcc atctgtctgt ccgtatccgg tgcgcctgca
    20761 tgggcatctg aacatcagtc cacgctgagc gcgggctatc ttcatgcctc gacgaacgtt
    20821 cccggcagcg atgatcttaa cgggattaac gtgaaatacc gttatgagtt tacggacaca
    20881 ctggggctgg tgacgtcatt cagctatgca ggagacaaga atcgccagct tacccgttac
    20941 agcgataccc gctggcatga agattccgtg cgtaaccgct ggttcagcgt gatggcgggg
    21001 ccgtctgtgc gcgtgaatga atggttcagc gcgtatgcga tggcgggtat ggcttacagc
    21061 cgtgtgtcga ctttctccgg ggattatctc cgcgtaactg acaacaaggg gaaaacgcac
    21121 gacgtgctga ccggaagtga tgacggtcgc cacagcaaca cgtctctggc gtggggagct
    21181 ggcgtgcagt ttaacccgac cgaatccgtg gccattgata ttgcttatga aggctccggc
    21241 agtggcgact ggcgcactga cggtttcatc gtgggtgtcg gttataagtt ctgattagcc
    21301 aggtaacaca gtgttatgac agcccgccgg ttcaggcggg cttttttgtg gggtgaatat
    21361 ggcagtaaag atttcaggtg tactgaaaga cggcacagga aaaccggtac agaactgcac
    21421 aatccagctg aaagcaaaac gtaacagcac cacggtggtg gtgaacacgc tggcctcaga
    21481 aaatccggat gaagccgggc gttacagcat ggacgttgag tacggtcagt acagcgttat
    21541 tctgttggtg gaaggattcc cgccgtcaca tgccgggacc atcaccgtgt atgaagattc
    21601 ccgacccggt acgctgaatg attttctcgg tgtcatgacg gaggatgatg cccgtccgga
    21661 ggcactgcgc cgttttgagc tgatggtgga agaggtggcg cgtaacgcgt ccgcggtggc
    21721 acagaacacg gcagccgcga agaagtcagc cagcgatgcc ggcacatcag cccgtgaggc
    21781 ggcaacccat gcgactgatg ctgcaggctc agcacgtgca gccagcacat cagccgggca
    21841 ggccgcgacg tcggctcagt cagcgtcttc cagcgcagga acggcatcaa caaaggctac
    21901 tgaagcatca aaaagtgctg ccgctgcaga gtcctcaaaa agcgcggcgg ctaccagtgc
    21961 cggtgcggcg aaaacgtcag aaacgaatgc cgcagcgtca caacaatcag cagccacttc
    22021 tgcatccacc gcgaccacga aagcgtcaga agctgccacc tcagcccggg atgcggcggc
    22081 ctcaaaagag gcagcgaaat catcagaaac gaacgcatcc tcgagcgcca gtagcgcagc
    22141 ttcctcggca acggcggcag gaaattccgc gaaggcggca aaaacgtccg agacgaacgc
    22201 caggtcttct gagacggcag cgggacagag cgcctcagct gcggcaggct caaaaacagc
    22261 ggctgcgtcg tctgccagta ccgcgtcaac aagtgccggg caggcctcag ccagtgccac
    22321 cgccgccgga aaatcggcag aaagtgccgc atcgtctgct tcaacagcca caacgaaggc
    22381 tggcgaagcc actgaacagg ccaacgcagc agcgaggtca gcttccgcag cgaagacatc
    22441 cgaaacgaac gcgaaagcgt cggaaaccag cgcagaatcc tcaaaaacgg ctgccgcatc
    22501 gtccgccagt tcggcggcgt catcggcctc atcggcgtct gcttcaaaag atgaggcgac
    22561 cagacaggca tcagcagcga aaggcagcgc cacgacggca tccacgaagg caacagaggc
    22621 tgctggcagt gcgacggcgg cagcacagag caagaatacg gcagaatccg cagcaacgcg
    22681 cgccgagaca gcagctaaac gggcagagga tattgcatcc gccgtggcgc ttgaggatgc
    22741 aagtacgacg aaaaagggga tagtacagct cagcagtgcg actaacagca cttccgagtc
    22801 actggcggca acgccaaaag ccgttaaggc cgcgtatgag ctggctaacg ggaaatacac
    22861 cgcacaggat gcaacgacag cacagaaagg gatagttcag cttagcaacg cgaccaacag
    22921 cacatctgaa atgctggcgg caacgccaaa gtcggtaaag gcagcctatg accttgctaa
    22981 cgggaaatat actgctcaga acgctacgac agcacaaaaa ggaattgtcc agctcagtag
    23041 tgcaaccaac agcgcatctg aaacgcttgc cgcgacaccg aaagcagtga aagcagctaa
    23101 tgataatgcg aatggtcggg taccttctgc ccgtaaggtg aatggtaagg cgctttcagc
    23161 ggatataaca ctgacgccga aagatattgg tacgcttaac tcaacaacaa tgtcattcag
    23221 cggtggtgct ggttggttca aattagcaac ggtaaccatg ccacaggcga gttctgttgt
    23281 ttcaattacg ttgattggtg gtgcgggatt taacgtgggg tcacctcaac aggcaggtat
    23341 atctgaactt gttttgcgtg caggtaatgg taatccgaag gggattactg gtgctttatg
    23401 gcagcgcaca tcgacagggt ttacaaattt tgcctgggtc aatacatctg gtgatactta
    23461 cgatatttac gttgcaatcg gaaattatgc gactggtgta aatattcaat gggattatac
    23521 cagtaatgcc agcgtgacga ttcatacgtc accagcatat tctgctaata agccggaagg
    23581 gttaacggac ggtacagttt attcactcta tacgccatca gagcagtttt atccgcctgg
    23641 cgcaccaatc ccgtggccat cagataccgt tccgtctggc tatgccctga tgcaggggca
    23701 gacttttgac aaatctgcat acccgaaact tgcagccgct tatccgtcag gcgtgatccc
    23761 tgatatgcgt ggctggacga ttaagggcaa acccgccagt ggtcgtgccg tattgtctca
    23821 ggaacaggac ggcattaaat cgcacaccca cagcgccagc gcatccagta cggatttggg
    23881 gacgaaaaac acatcgtcgt ttgattacgg aaccaaatcc acgaataaca ccggggcgca
    23941 tacccatagt attagcggga ctgcaaatag tgccggtgcg caccaacaca agagttccgg
    24001 tgcatttggt ggcacgaaca cgagcatttt ccctaatggt tataccgcga tttcaaatct
    24061 aagcgcgggg attatgagca caacaagcgg tagtggccag actcgtaatg cagggaagac
    24121 atcatcagat ggtgctcata cccactcgct gtccggcact gctgcaagcg caggcgcaca
    24181 tgcacatact gtcggtattg gtgctcatac gcactccgtt gcgattggtt cacatggaca
    24241 caccatcacc gttaacgctg ctggtaacgc ggaaaacacc gtcaaaaata tcgcatttaa
    24301 ctatattgtg aggcttgcat aatggcattc agaatgagtg aacaagcacg gaccataaaa
    24361 atttataatc tgctggccgg aactaatgaa tttattggtg aaggtgacgc atatattccg
    24421 cctcatacag gtctgccagc aaacagtacc gatattgcac cgccagatat tccggctggc
    24481 tttgtggctg ttttcaacag tgatgaggca tcgtggcatc tcgttgaaga tcatcgggga
    24541 aaaaccgtct atgacgtggc ttccggcgac gcgttattta tttctgaact tggctcgtta
    24601 ccggaaaatg tcacctggtt atcgccggaa ggggaatatc agaagtggaa cggcacagcc
    24661 tgggtgaagg atacggaagc agaaaaactg ttccggatcc gggaggcgga agaaacaaaa
    24721 aacagcctga tgcaggtagc cagtgagcat atagcgccac ttcaggatgc tgtagatctg
    24781 gagatcgcaa cggaggaaga aaactcgttg ctggaagcct ggaaaaagta tcgggtgttg
    24841 ctgaaccgtg ttgatacatc aactgcaccg gatattgagt ggcctgctgt ccctgttatg
    24901 gagtaatcgt tttgtgatat gccgcagaca cgtcgtatgc agaaacgtgc tgcggctggc
    24961 tggtgaactt tcaatagtgc gagtattgaa tgatttccag ctgttatcga ttttatgtgt
    25021 tttttgcatg aggggatttc caccacctcc caccgaccat ctaagatttt atgccactgt
    25081 ccctaggact gctatgtact aggagcggat gttaaactca gactcgtttc agctacattg
    25141 cgttttgaat aatattccat cataataact ctttgaaaaa tgtgatcttt tcatttataa
    25201 cactgatgac ttgcttatct cattgggata tcggaggaga atacttaact atgacaagcc
    25261 cgattattat gacactggct atattatata gattgatatt aaaatgtagg gttaggttct
    25321 tgccaaggtg tcaagattta cagataggtt taaaaccata taaatatgtt ttacggtgag
    25381 atacaataca tattgtaagg cataaacgct tggtaaaatt ttaattattg gaagaagcta
    25441 atcatggaac ccatatcaat tacagtggca acttatgtag caactaaact tattgatcaa
    25501 ttcatctctc aagaaggata tggttgtatt aagaaagcat tattccccca aaaaagatat
    25561 gtggatagat tatatcaact aattgaagag acggcaattg agtttgaaga aacatatcca
    25621 gtagaaagtg gagcaatacc attttatcat tccgaaccat tgtttgagat gttgaatgag
    25681 cacatctttt ttaaagagtt ccctgacaaa gagatattat tagacaagtt caaagaatat
    25741 ccaagtatca ctcccccaac tcaacaacaa ctcagccttt tttatgagat gttatcatta
    25801 aaaatcaata attgttcgaa gttaaaaaag ctacatatcg aagaaacgta taaagaaaaa
    25861 atattcgata ttaatgaaga gctcattcaa gttaaactta ttttacggtc tatagatgag
    25921 aaactaactt ttcacttaag tgatgattgg ttaaatgaaa aaaatagtca agcaatagct
    25981 gacttgggag gtcgatacac acccgaactc aacgtaaagc tagaaatagc agagatattt
    26041 gatggcctcg gtagaactaa tgatttttct aaaatatttt attcgcatat agatagtttt
    26101 ctggtcgctg gaaagaaatt acatagttgc gatgtaattt cctcagaatt atttgaaata
    26161 aaccagtcct taaaagaaat ttctgatata tatcaggaga ttaatttttc taaattagat
    26221 gaaatcccta taaataaatt taataactat gtttctagct gccagacagc tattggcgga
    26281 gcggtatcaa tattgtggga actccgagaa aagtcagagc aagtaggtga aaccaagcat
    26341 tacagtgata agtattcatc tactctgcga atgcttcggg aatttgacta tgcgtgcaat
    26401 gaattacgta tattcattaa ttcaacaaca gtgaagttgg ctaacaaccc attcttactt
    26461 ctcgaaggaa aagcaggaat tggtaagtct catttactgg ctgatgtgat taaaaatcga
    26521 attacttctg ggtatccttc actactcata ctagggcaac aacttacttc agatgaatct
    26581 ccatggtcac aaatcttcaa gagattacag cttaaaatca cttctcgtga attcctagaa
    26641 aaactgaatt tatatggcaa aaaaatagga aaaagagtct tagtttttat tgatgctatt
    26701 aatgaaggta atggaaataa attctggaat gacaatatta acagttttgt cgatgaaatc
    26761 agatgctttg aatggcttgg tctgataatg tcagtcagaa caacatatag aaatgtaaca
    26821 atttcacatg agaatgttgt gcgaaataat tttgaaattc atgaacatat tggattccag
    26881 aacgttgagt tggatgcggt tagtctattt tatgattatt acaatattga gaggccttca
    26941 tctcctaacc ttaatccaga gtttaaaaat cctctatttc ttaagttatt gtgtgaaggc
    27001 attaagaaaa atggtttaac caaagtgcct gttggattta atgggatttc aaatattttt
    27061 aactttttag tggaaggggt aaataaatca ttagcatcgc caaaaaaata tgcattcgat
    27121 cccagttttc ctcttgttaa agatgctctc aatgaaatca taaaattcaa attagagatt
    27181 ggtcgtaata gtatttcact taaagatgct cactcagtgg ttcaatctgt agttaatgat
    27241 tatgttgctg ataaaacctt cctcagcgcc ttgattgacg aaggattatt gactaaaggc
    27301 atagtgagaa atgatgataa ttctactgag gaagtagttt atgtggcttt tga
//
LOCUS       DAEEKQ010000040        27234 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.040, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000040 DAEEKQ010000000
VERSION     DAEEKQ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27234
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.040"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            182..463
                     /locus_tag="KLK57_001643"
     CDS             182..463
                     /locus_tag="KLK57_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892031.1"
                     /translation="MTEAEILGLIRRVAGISQQVDEQAMQPDSVTAENYARVVDEVMR
                     RDGIELNGVDMRNIRTRVLELLAYRRRSQQRRESAKNTYQWRKPEHLRR"
     gene            1103..1423
                     /gene="clpS"
                     /locus_tag="KLK57_001644"
     CDS             1103..1423
                     /gene="clpS"
                     /locus_tag="KLK57_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HBE0892032.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            1454..3730
                     /gene="clpA"
                     /locus_tag="KLK57_001645"
     CDS             1454..3730
                     /gene="clpA"
                     /locus_tag="KLK57_001645"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HBE0892033.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(4136..4223)
                     /locus_tag="KLK57_001646"
     tRNA            complement(4136..4223)
                     /locus_tag="KLK57_001646"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4187..4189),aa:Ser,seq:gga)
     gene            complement(4477..4695)
                     /gene="infA"
                     /locus_tag="KLK57_001647"
     CDS             complement(4477..4695)
                     /gene="infA"
                     /locus_tag="KLK57_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HBE0892034.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(4980..5684)
                     /gene="aat"
                     /locus_tag="KLK57_001648"
     CDS             complement(4980..5684)
                     /gene="aat"
                     /locus_tag="KLK57_001648"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HBE0892035.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGAPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWHEDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFISHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQK"
     gene            complement(5726..7447)
                     /gene="cydC"
                     /locus_tag="KLK57_001649"
     CDS             complement(5726..7447)
                     /gene="cydC"
                     /locus_tag="KLK57_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HBE0892036.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGGAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTVGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(7448..9214)
                     /gene="cydD"
                     /locus_tag="KLK57_001650"
     CDS             complement(7448..9214)
                     /gene="cydD"
                     /locus_tag="KLK57_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HBE0892037.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWAVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEVELASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEHELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGQIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(9337..10302)
                     /gene="trxB"
                     /locus_tag="KLK57_001651"
     CDS             complement(9337..10302)
                     /gene="trxB"
                     /locus_tag="KLK57_001651"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HBE0892038.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            10847..11341
                     /gene="lrp"
                     /locus_tag="KLK57_001652"
     CDS             10847..11341
                     /gene="lrp"
                     /locus_tag="KLK57_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HBE0892039.1"
                     /translation="MVDSKKRLGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            11476..15543
                     /gene="ftsK"
                     /locus_tag="KLK57_001653"
     CDS             11476..15543
                     /gene="ftsK"
                     /locus_tag="KLK57_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HBE0892040.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTAAQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQPQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPVQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVETAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVAPQQQYQQPQQPVAPQPQYQQPQQPV
                     APQQQYQQPQQPVAPQPQYQQPQQPVAPQQQYQQPQQPVAPQPQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            15698..16309
                     /gene="lolA"
                     /locus_tag="KLK57_001654"
     CDS             15698..16309
                     /gene="lolA"
                     /locus_tag="KLK57_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HBE0892041.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            16320..17663
                     /gene="rarA"
                     /locus_tag="KLK57_001655"
                     /gene_synonym="mgsA"
     CDS             16320..17663
                     /gene="rarA"
                     /locus_tag="KLK57_001655"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HBE0892042.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLGTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            17754..19046
                     /gene="serS"
                     /locus_tag="KLK57_001656"
     CDS             17754..19046
                     /gene="serS"
                     /locus_tag="KLK57_001656"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HBE0892043.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            19285..21729
                     /gene="dmsA"
                     /locus_tag="KLK57_001657"
     CDS             19285..21729
                     /gene="dmsA"
                     /locus_tag="KLK57_001657"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HBE0892044.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPEWASQITGVPADKIIKLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLVNQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            21740..22357
                     /gene="dmsB"
                     /locus_tag="KLK57_001658"
     CDS             21740..22357
                     /gene="dmsB"
                     /locus_tag="KLK57_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBE0892045.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            22359..23222
                     /gene="dmsC"
                     /locus_tag="KLK57_001659"
     CDS             22359..23222
                     /gene="dmsC"
                     /locus_tag="KLK57_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HBE0892046.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDSWAMRLLPAISVLALVVSGVVSVMQGAELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(23258..23884)
                     /locus_tag="KLK57_001660"
     CDS             complement(23258..23884)
                     /locus_tag="KLK57_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE0892047.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            24199..25347
                     /locus_tag="KLK57_001661"
     CDS             24199..25347
                     /locus_tag="KLK57_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0892048.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            25557..26987
                     /locus_tag="KLK57_001662"
                     /pseudo
     CDS             25557..26987
                     /locus_tag="KLK57_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
     gene            complement(26988..>27234)
                     /locus_tag="KLK57_001663"
     CDS             complement(26988..>27234)
                     /locus_tag="KLK57_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0892049.1"
                     /translation="DVDAELDAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWITERP
                     GFQIYYPNRQYMSCGLRAFLDYVKTGQICQSQRHRPQ"
BASE COUNT         6761 a         6651 c         7251 g         6571 t
ORIGIN      
        1 aaaaaagcgc atttttgtgg ttttatcgtc atcatcatca tgtgtgtaac ctgttgtttt
       61 taatgtagtt gatgcaaaaa agatgatgat tgtggttaat ttttgttcga catcttttag
      121 cgtgacagat tctttacaaa aaatctgagc ttgttttctt aaccagcgcg atgggggcac
      181 aatgacagaa gccgaaatac tgggattaat ccgccgcgtc gccggaatca gccagcaggt
      241 tgacgaacag gccatgcaac cggacagcgt gaccgctgaa aattatgcgc gtgtggtgga
      301 tgaggtaatg cgccgtgacg gtattgagct taacggcgtg gatatgcgca acatacgaac
      361 cagagttctt gagttgctgg cataccgtcg ccgttctcaa caacggaggg agagcgcgaa
      421 aaatacttac cagtggagga agccggagca cttacggcgg taactggttg atattaccga
      481 aagtgcaaaa atgcacttag caactggtgg cgagttgcag atctgcaact cgactatgaa
      541 actacggaaa ctacccgtag tttgggtagt aagagtaaca cccagatttt ggggcttact
      601 cgcgataccc aaataaaggg tatcggtgga agaaatatcg tttctcatat gtgagtaccg
      661 agggcggaat tccgccttcg gttacttatt gtgcgcatgc acagggaggg gcgggtcaaa
      721 tctctgtacc ctgacgtctt ccggactgcc agccccatcg attttttata cccgcgaaaa
      781 atgaaaaacg cttcacgctg gtgggcctga tgccgataac tggtttcatg actgatagcc
      841 ggaaaatgat aacgattaaa gcgtgttgat ctttgttcgt ggttgttcga tattgttcgt
      901 ggaacggtgt agttaatggt gtagttaatt ttactgtttt tgacgagctt attgtttgca
      961 actcaatgaa aaataaaggc taataagcca aatgctataa atgatagtta tctatcatgt
     1021 ggagtagatt ggtcaggcaa ataagctctt gtcagcggca gggcgttctg ccgataaccg
     1081 taaccgaaga tgataactga caatgggtaa aacgaacgac tggctggact ttgatcaact
     1141 ggcggaagaa aaagttcgcg acgcgctaaa accgccatct atgtataaag tgatattagt
     1201 caatgatgat tacactccga tggagtttgt tattgacgtg ttacaaaaat tcttttctta
     1261 tgatgtagaa cgtgcaacgc aattgatgct cgctgttcac taccagggga aggccatttg
     1321 cggagtcttt accgccgagg ttgcagaaac caaagtggcg atggtgaaca agtacgcgag
     1381 ggagaatgag catccattgc tgtgtacgct agaaaaagcc tgaatgcagg cataaaaatt
     1441 gggggaggtg cctatgctca atcaagaact ggaactcagt ttaaatatgg ctttcgccag
     1501 agcgcgcgag caccgtcatg agtttatgac cgtcgagcac ttgttactgg cgctgctcag
     1561 taacccatct gcccgggagg cgctggaagc gtgttctgtg gatttggttg cgctccgtca
     1621 ggaactggaa gcctttattg aacaaaccac acccgttctg cctgccagtg aagaggagcg
     1681 cgacacacag ccgacgctga gttttcagcg tgtactgcaa cgtgcggtct tccatgtcca
     1741 gtcctccggt cgcaatgagg ttaccggtgc aaacgttctg gtcgctatct ttagcgaaca
     1801 ggagtcgcag gcggcatatc tgttgcgtaa acatgaagtc agccgtctcg atgtggtgaa
     1861 ttttatctct catggcacgc gtaaagacga gccgacacag tcttctgatc ctggcagcca
     1921 gccaaacagc gaagaacaag ctggtgggga ggaacgtatg gagaatttca cgacgaacct
     1981 gaatcagctt gcgcgcgtgg gcggaatcga cccactgatt ggtcgtgaga aggagctgga
     2041 gcgtgctatt caggttctct gccgtcgccg taaaaacaac ccgctgctgg tgggggaatc
     2101 tggtgtcggt aaaaccgcga ttgcggaagg tcttgcctgg cgaattgttc agggcgatgt
     2161 gccggaagtg atggctgact gtacgattta ctctctcgat atcggttctc tgttagcggg
     2221 cacaaaatat cgcggcgact ttgaaaaacg ttttaaagcg ttgctcaagc agctggagca
     2281 ggacactaac agcatcctgt ttattgatga gatccacacc attatcggtg cgggggcagc
     2341 gtctggtggt caggttgatg cggctaacct aatcaaaccg ttgctctcca gcggtaaaat
     2401 tcgtgtaatt ggttcgacaa cctatcagga gttcagcaac attttcgaga aagaccgtgc
     2461 tctggcgcgt cgcttccaga aaattgatat tactgaaccg tcaatcgaag aaactgttca
     2521 aatcatcaat ggcctgaaac cgaagtatga agcgcaccac gacgtgcgtt ataccgcaaa
     2581 agcggtgcgt gcggcggtag agctggcggt gaaatacatc aatgatcgtc atctgccgga
     2641 taaagcgatt gatgttatcg acgaagcggg cgctcgcgca cgcctgatgc cggtaagcaa
     2701 acgcaagaaa accgttaatg tggcggatat tgagtccgtg gtggcccgta ttgcacgcat
     2761 tccagagaag agtgtttctc agagtgaccg cgataccctg aaaaacctcg gcgatcgcct
     2821 gaaaatgctg gtcttcggtc aggataaagc cattgaggcg ctgactgaag ccattaagat
     2881 ggcgcgtgca ggtttaggtc acgaacataa accggtcggt tcgttcctgt ttgccggtcc
     2941 taccggggtc gggaaaacag aggtgacggt acagctttcg aaagcgttgg gcattgagct
     3001 tctgcgcttt gatatgtccg agtatatgga acgccatacc gtcagccgtc tgattggtgc
     3061 gcctccggga tacgttggtt ttgatcaggg cggtttgctg accgatgcgg taatcaagca
     3121 tccacatgcg gtgctgctgc tggacgaaat cgagaaagcg catccggacg tgttcaatat
     3181 tctgttgcag gtgatggaca acggtacgct gaccgataac aacggacgca aagcggactt
     3241 ccgtaacgtg gtgctggtga tgaccaccaa cgccggggta cgggaaactg agcgcaaatc
     3301 cattggtctt atccaccagg ataacagcac cgatgcgatg gaagagatca agaagatctt
     3361 tacaccggaa ttccgtaacc gtctcgacaa cattatctgg tttgatcatc tgtcaaccga
     3421 cgtgatccat caggtggtgg ataaattcat cgtcgagttg caggttcagc tggatcagaa
     3481 aggtgtttct ctggaagtga gccaggaagc gcgtaactgg ctggccgaga aaggttacga
     3541 ccgggcaatg ggcgctcgtc cgatggcgcg tgtcatccag gacaacctga aaaaaccgct
     3601 cgccaacgaa ctgctgtttg gttcgctggt ggacggcggt caggtcaccg tcgcgctgga
     3661 taaagagaaa aatgagctga cttacggatt ccagagtgca caaaagcaca aggcggaagc
     3721 agcgcattaa tctaattgtt aggtaggttg gtgaagtccg taatctcgaa agaggttacg
     3781 gactttttgt ttatggggtg gaacggtgaa aaccctattt ttggaggtga aggtaatttg
     3841 ttgataatta gtgctgctgg aaaaggtaag gataaaaaag ggtgacagca ggaaaatgag
     3901 atggttttgc tttattaaca acggactaaa cgtgtagtat ttgagttcac tgccgtacag
     3961 gcagcttaga aatcgacggg gtgcggtaaa acctttgcga acgcgttcac tgccgtacag
     4021 gcagcttaga aattcacagg taacatactc cacccgccca ccatgttcac tgccgtacag
     4081 aaagataaaa tgcgaaaaaa aagctcgcac tttcgtacga gctcttcttt aaatatggcg
     4141 gtgagggggg gattcgaacc cccgatacgt tgccgtatac acactttcca ggcgtgctcc
     4201 ttcagccact cggacacctc accaaattgt tttgctacca aacctcatgg gtggcaacgg
     4261 ggcgctacta tagggagttg gagtaaaacg gtcaagaaga attttaatga taattattgt
     4321 ttgctcatac tgtaaacaag ttgtgcagta tatctacatc gagacaagtt acggacttat
     4381 acttccaaag tacttcatac atatcacaaa ataaaaaggc cggttaaacc gaccttttac
     4441 tcgttctttc tcttcgccca tcaggcggta aaacaatcag cgactacgga agacaatgcg
     4501 gcctttgctc aggtcgtacg gggtcagttc aacagtcact ttgtcgcccg tcaggatgcg
     4561 gatgtagttt ttgcgcattt taccggagat gtgtgcagta accacgtgac cgttttctaa
     4621 ctctacgcgg aacatggtat taggcaacgt ttcaagaacg gtaccttgca tttcaatatt
     4681 gtcttctttg gccatctaat cctctggggt atcactaccg taatttgaac cggcaagata
     4741 atgccgaagt tctgtaaata agtaaagatt tgcgcgctaa atcgcaacaa acaggttcgg
     4801 cacattactc cgaaaacaca cggctaagcc gcaccaaaag cgcaacgtat aagggagcgg
     4861 tgagataaac gatgggcgtt acctgacgcg aaaaattcct tatcggcagc ggggtaatga
     4921 gcataaccaa ctctgcgacc gcaattataa cactctgggg agaaatgtgc cgaaaacatt
     4981 catttttgtg gtgaaaacaa gcatcgtggc acccagaaat tattcggcaa tcgtccgagg
     5041 cgcatttgat tgagataatt cagataatcc cgacggggaa tttcgcaggc accaagcgat
     5101 gctgtgtgat cgttaaggac ctggcagtcg ataagcttac cgccatgact gataaattcc
     5161 tcacagaata ccagaagcgc cgttttagac gcattttcca tccggctgaa catggactcg
     5221 ccacaaaata gcgttccctg ggccacgccg tacataccgc cgacaagctc atcttcatgc
     5281 cagacttcaa tggagtgggc atgaccgagt tcgtgaaggc gatggtaggc ttcgaccacg
     5341 ccacgcgtga tccaggttcc ttcttcgcga tcgctggcac aaccttcaat gacctgacca
     5401 aaagcgtaat tcatcgtgac acgatagggc gagcgtttat gaaatcgctt catactacgg
     5461 ctgatatgca gtgattcagg ccaaagcacc gcgcgaggat cgggcgacca ccagaggatg
     5521 ggggcgcctg gagaaaacca cggaaaaata ccacgctggt aagccattaa caggcgcgca
     5581 gggctaagat cgccccccag agccagcagg ccgttaggct cacgtaatgc gccttccggg
     5641 gaagggaagg ctattgaatg gcgagaaagc tgaaccaggc gcatgaccgc aaaactccac
     5701 gcaagtcgga tcgttcaata atagcttaca aaccctgctt aaactggtaa taacgcccct
     5761 gtctggcaag cagttctgcg tgagtacctt gctcaataat ttgcccgttg tccatcacta
     5821 ttatttgttg gaaacgagag agtccgcgaa gtcgatgggt gaccattaac accgttttct
     5881 cacgcatcat ttctgcaagc aattcaagga tctggctttc ggttgtggca tctaagcctt
     5941 cggtaggttc atccagcaac accagtggcg catcatgtaa cagcgcacgg gcgatagcca
     6001 gacggcgcag ttcaccaccg gagagctggc gtccgccttc acctaaccaa ctgttgagac
     6061 ctgcgtcctc gagcagcttc tccagaccaa cgcgacgcaa gatctccgac agagcctcat
     6121 cactactgcc aggcgaggcg agtaacagat tatcacgcag cgtggcgcta aacagatgca
     6181 ctcgctgagg aacaacgctg atggtctgtc gtagagccgc ttcattcagg ctggctatgg
     6241 ggctatcgtt aagcaaaatc tcgccctgtt gcgggtccca agcgcgggtc agcagttgta
     6301 acagtgttga tttgccgcat ccggttcgcc cgagaatcgc aatatgttcc ccggcgttta
     6361 cctgaagaga aatcccttta agtgcctgtt gggattgctc cggataagtg aactgaacat
     6421 cccgtaatgt cagcgaaacg cgatcggcaa cacgagtttg ggtatcaggg aaggtgactt
     6481 ccggtttttg atccgttaaa tcagtgatcc gaacggcaga ggcaatgact tgccccagat
     6541 gctgaaatgc acccgttact ggtgccagtg cttcaaacgc ggctaacgcg cagaagacaa
     6601 acagggcaat taacgcgccg ggttgagcat tgccgccaac gccgccagac gccatccaca
     6661 gcatcaggat caccgctaac gcgccaatga gcagcattat cgcttgcgac aatgcggtca
     6721 gttcagattg acggcgttgc gcttccagcc attgaatttc tgtattctct agttgcgtgc
     6781 gataacgatc gctggcacca aaaatggtca gctcagcttg cccttgcagc caggccgtca
     6841 gttgttggcg atactgtccg cgaagatgag tcagattttg cccggtgctt tttcccgcac
     6901 gataaaacag cggtggcatc aggaaaagcg tcagtaacat aatgccgccc agcgtaaagg
     6961 cgagggtgaa atcaaggaaa cttaacccga ctgtcaccac cataatcacc acaaaagcgc
     7021 ccaccagcgg cgagataacg cgcaggtaaa gatgatcgag cgtatcaaca tccgccacca
     7081 cgcgattgag caattcgccc tgacgatagc gcgccagtcc ggcaggggag aggggcaaca
     7141 atttgctgaa ggtgtaaatg cgcagatgct gcaacacgcg gaaagtcgcg tcgtgactta
     7201 ccagacgttc aaaataacgc ccggcagtac gggtgattgc ggcaccacgc acgcccgcag
     7261 cgggtagcat atagttgaag ctgtacagtc cggcaccccc cgcaaccgct gaggccgaga
     7321 ggaaccagcc ggaaagtgtc aacagaccga tactggcgag cagcgtcaca attgccagca
     7381 caataccaag acttaacatc catttatgac gtttatacag tgccagatag ggtagcaaag
     7441 cgcgcattta aatctcctcc tgacgatggg ccagtaatgt ggcgaatggg ccaccagcca
     7501 cacttaattc cgcgtaacgt ccttgctcaa taatctgacc atcctgcatt acccaaatga
     7561 catcccagtc agcaagatct tctaactggt gggtgaccat taacgttgtc tggcgcagag
     7621 aggcggcatt cagcgcctcc attacgcgct gttcactgtg agcatcaagg ctggcagcgg
     7681 gttcatccaa cagtaatagc gaacagggat ttagtaacgc acgggccacc gccacgcgct
     7741 gcgcctgccc cacggaaagg cgggcagcct ggtcgccaac aggcgtatca acgccttgtg
     7801 gcaggagcgg tagaaactcg ctgacccagg cgttatccag cgctgcttgt aattcatgtt
     7861 cgctggcatc aggtcgcgcc agtagtacgt tatcccgtaa tgttgctgcc ggtaattgtg
     7921 gattttgccc aacccaggag agatgtttac gccatgattc tggtgataaa tcgcgtaatt
     7981 ctatcccgtt gattcgtagc gatccctgat atgagagaaa accagaaagc gcgttcaaca
     8041 gtgaactttt acctgaaccg ctgcgaccaa ccaacacggc acgttggcct gctggcaaag
     8101 taaagttcag cggtccggcc agcgttttac cttccggcga cgtgataaac agatcctcgg
     8161 cctcaatggt caccggatcg gtcgatgcta attccacctc gccacgctgc ggatgggcga
     8221 gcggggtttc cataaacgtt ttcagactgt cagctgcacc aacagcctgg gctttagcat
     8281 gataaaacgt accgagatcg cgtaatggct ggaaaaactc tggcgcaagg atcagggcca
     8341 gaaaacccgc agccagcgtc acgccagtat cgtagtgacc aaaatccagc tcgccgagat
     8401 aggaaaaacc aaagtagacc gccaccagag caattgacag cgaggtaaaa aattcgagaa
     8461 tgccggagga taaaaatgcc agccgtagca cttccattgt ccgttggcgg aaatcttccg
     8521 aagcagaacg aatactttca atttcagctt caccacgacc aaaaatacgc aatgtttcca
     8581 tgccgcgcag gcgatcgagg aaatgcccac ttaagcgagc aagagcgaga aagttacgtc
     8641 ggttagcatc ggcagccccc attccaacca gcgccataaa caacggaatt aacggtgcag
     8701 tgcccagcag aatgagcgcc gcagcccagt ttgaggggaa gattgccacc acaatcagca
     8761 acggcaccga cactgccagc gccatttgcg gcaggtagcg tgcatagtaa tcatgcatat
     8821 cgtcaatttg ctcgagtacc agcgtcgccc agctccccgc aggtttaccc tgaatccacg
     8881 ctggccctgc ttgttgcaga cggtcgagaa cctgacggcg gatggcaaag cggatatgct
     8941 gcccggcgtg ataacccacc cgttcgcgta accagaccgc ccatgcgcgc agtacaaagg
     9001 tcagaaccag taacgtaaag ggaagcagca gggcttcacg gggaatattc tccataatca
     9061 tatgttgcag aattcgcgcc atgaaccagg cctgggcaat gatcaatatg ccgcttacaa
     9121 agcccagcag acgagaaata ttcagccaac gttgggagat gacgctttgc tgttttaacc
     9181 agcgggttaa ctctttttga cgagatttat tcattgcacg cttagcaggt gagttatcag
     9241 aattatttgc agagcaatgt tacaacgggg aaaaaataaa ggcgacccat agtcgcaagg
     9301 tgtcgccttc tttacttttg ttactgattt gtaaaattat tttgcgtcag ctaaaccatc
     9361 gaggtagcgt tccgcatcaa gtgctgccat gcagccagta ccggccgaag taatggcctg
     9421 gcgataaatg tgatccatca cgtcgcctgc ggcaaagacg ccaggaatgc tggtctgggt
     9481 ggcattacca tgaatacccg actgtacttt gatgtagccg ttttccagtt ccagctgccc
     9541 ttcgaaaatc gcagtattcg ggctgtgacc gatagcaaca aacagaccgg caacgtcgag
     9601 tgactcgatg ttatcgctgt tttgcgtatc gcgcagacga acgccagtga cacccatttg
     9661 atcgccggtc acttcttcca gcgtacggtt ggtgtgcaga atgatgttgc cgttctccac
     9721 tttatccatc agacgcttaa taaggatttt ttccgcgcgg aaaccgtcac ggcggtgaat
     9781 cagatgcact tccgaagcga tgttagacag atacagcgcc tcttcaaccg cggtattgcc
     9841 gccgccgatg accgcaactt tctggttgcg atagaagaaa ccgtcgcagg ttgcacaagc
     9901 agaaacccca cggcctttaa aggcttcttc agagggcagg ccgagatagc gtgcagaagc
     9961 tccggtggca ataatcagcg cgtcgcaagt gtattcgccg ttatcgccat tcagacggaa
    10021 cggacggttt tgcagatcca ccttgttgat atgatcaaaa atgatctcag tttcaaactt
    10081 ggtggcatgt tcgtgcatgc gctccattaa taacggaccg gtcagatcgt ttggatcacc
    10141 aggccagttt tccacttccg tggtggtggt cagttggccg cctttttcca tgccggtaat
    10201 cagcacaggt tgcaggttgg cgcgcgccgc gtagacagca gcggtgtatc ccgccgggcc
    10261 tgaacccagg ataagcagtt tactgtgttt ggtcgtgccc atgagatccc catagttgtt
    10321 ggcagacaat gggcaggatt gtagggaatt tacagacgta aaaaaagagt atgacgattt
    10381 tgttaacaat ttgtgcaatc ggcagcatcg ataagcaggt caaattctcc cgtcattatc
    10441 acctctgcta cttaaatttc ccgctttata agccgattaa atgatgaata aacgcccctg
    10501 ttaatgaata tctggcatgt tgtactaaaa atcgatgttt tgctttgaca atcccctggt
    10561 gttttgcgaa aacattcgag gaagaaaaaa aacagtattc ttatatgcgc ataaccatgc
    10621 atgtaaatac catgtttacc gtgctagtga aatctacgta tggcgtggac agacgccatt
    10681 cgtgatgtcg atagctgcca caaggcaacg gtcttctcac cgtagaccca ggcattgcgc
    10741 gccgtgaatc ttcatgattt cggtctatcg tgacgggtag cgactctgaa cagtgatgtt
    10801 tcaggggcag acaggagtag ggaaggaata cagagagaca ataataatgg tagatagcaa
    10861 gaagcgcctt ggcaaagatc tcgaccgtat cgatcgtaac attcttaatg agttgcaaaa
    10921 ggatgggcgt atttctaacg tcgagctttc taaacgtgtg ggactttccc caacgccgtg
    10981 ccttgagcgt gtgcgtcggc tggaaagaca agggtttatt cagggctata cggcgctgct
    11041 taacccccat tatctggatg catcacttct ggtattcgtt gagattactc tgaatcgtgg
    11101 cgcaccggat gtgtttgaac aattcaatac cgctgtacaa aaacttgaag aaattcagga
    11161 gtgtcattta gtatctggtg atttcgacta cctgttgaaa acacgcgtgc cggatatgtc
    11221 agcttaccgt aagttgctgg gggaaaccct gctgcgtctg cctggcgtca atgacacacg
    11281 gacatacgtt gttatggaag aagtcaagca gagtaatcgt ctggttatta agacgcgcta
    11341 acacggaaca ggtgcaaaat cggcgtattt tgattacact cctgttaatc catacagcaa
    11401 cagtactggg gtaacctggt actgttgtcc gttttagcat cgggcaggaa aagcctgtaa
    11461 cctggagagc ctttcttgag ccaggaatac actgaagaca aagaagtcac attgacaaag
    11521 ttaagtagcg gccgccgcct tctggaggcg ttgctgatcc ttattgtcct gtttgccgtc
    11581 tggttgatgg ctgccttact aagctttaac ccttcggacc ccagctggtc gcaaacggcc
    11641 tggcatgaac ctatccataa tttaggtggg atgcccggtg cgtggttggc agatacgctg
    11701 ttctttattt ttggcgtgat ggcttacacc attcccgtca ttattgtcgg cggttgttgg
    11761 tttgcctggc gtcatcagtc cagcgacgaa tacattgatt attttgccgt ttcgctacgc
    11821 atcattggcg ttttggcgct catccttacc tcctgtggtc tggcggcaat caacgctgac
    11881 gatatctggt attttgcctc cggtggcgtc attggcagct tactaagcac tacgctacaa
    11941 ccactgctac acagtagcgg gggaactatt gcgctgctct gcgtttgggc agcgggcctg
    12001 acgttgttca ccggttggtc atgggtgacc attgctgaaa aactcggcgg ctggatttta
    12061 aacattctca ccttcgccag taatcgtacc cgtcgcgatg atacctgggt cgatgaagat
    12121 gagtatgaag acgacgaaga gtatgaagat gaaaatcacg gcaaacagca tgaatcacgc
    12181 cgtgcccgta ttcttcgcgg cgcgctagcg cgtcgtaaac ggttggcgga aaaattcatt
    12241 aatccgatgg ggcggcaaac agacgctgcg ttgttctccg gtaagcggat ggatgatgac
    12301 gaagagatta cctacactgc acgcggtgtg gctgccgacc ctgacgacgt cctattttcg
    12361 ggcaatcgtg caacgcagcc agaatatgac gaatacgatc cattattaaa cggtgcgcca
    12421 attaccgaac ctgtcgctgt agcagctgct gctaccacgg cggcacaaag ctgggctgcg
    12481 ccggttgaac ctgtgactca gacgccgcct gttgcctctg ttgatgttcc accttcgcaa
    12541 cctacagtag cctggcagcc tgtaccgggt ccacaaacgg gagagccggt tattgctcct
    12601 gcaccggaag gttacccaca gcagccacaa tatgcgcagc ctgcagtgca atataatgag
    12661 ccgctgcaac aaccagtaca gccacagcag ccgtattatg cacctgcagc tgaacaacct
    12721 gtgcaacaac cttactacgc tcctgcggcg gaacaacctg ttcagcaacc gtattacgcc
    12781 cctgcgccag aacaaccggt ggcaggtaac gcctggcaag ccgaagagca gcaatccact
    12841 tttgctccac agtctacata ccagactgag caaacttatc agcaaccagc cgctcaggag
    12901 ccgttgtacc aacagccgca acccgttgaa cagcagcctg ttgtggagcc tgaacccgtt
    12961 gtagaagaga caaaacccgc gcgtccgcca ctttactatt ttgaagaagt ggaagagaag
    13021 cgagcccgtg aacgtgaaca acttgcggcc tggtatcaac cgattccaga accggttaaa
    13081 gaaccagaac cgatcaaatc ttcgctgaaa gcaccttctg ttgcagcagt acctccagta
    13141 gaaaccgctg ccgctgtttc tccgctggca tctggcgtga aaaaagcgac actggcgacg
    13201 ggggctgccg caaccgttgc cgcgccagtc ttcagtctgg caaatagcgg tggaccgcgt
    13261 ccgcaggtca aagaggggat tggcccgcag ttgccacgac cgaaacgtat ccgcgtgcca
    13321 actcgccgtg aattggcgtc ttacggtatt aagctgccct cacagcgtgc ggcggaagaa
    13381 aaagcccgtg aagcccagcg caatcagtac gattctggcg atcagtacaa cgatgatgaa
    13441 atcgatgcga tgcagcagga tgaactggca cgtcagttcg cccagacaca gcagcaacgc
    13501 tatggcgaac agtatcaaca tgatgtgccc gtaaacgcag aagatgcaga tgctgcggca
    13561 gaggctgaac tggctcgtca gtttgcgcaa actcaacaac aacgttattc cggcgaacaa
    13621 ccggctgggg cgaatccgtt ctcgctggat gattttgaat tttcgccaat gaaagcgttg
    13681 ctggatgatg gtccacacga accgttgttt acgccaattg ttgaacctgt acagcagccg
    13741 caacaaccgg ttgcaccgca gcagcagtat cagcagccac aacagccggt tgcgccacag
    13801 cagcaatatc agcagccgca acagccggtt gcgccgcagc cacaatatca gcagccgcaa
    13861 caaccggttg cgccacagca gcaatatcag cagccgcaac aaccagttgc gccgcagccg
    13921 cagtatcaac agccgcaaca accggttgcg ccgcagcagc agtatcagca gccacaacag
    13981 ccagttgcgc cacaaccgca ggataccctg cttcatccgc tgttgatgcg taatggcgac
    14041 agccgtccgt tgcataaacc gacgacgccg ctgccttctc tggatttgct gacaccgccg
    14101 ccgagcgaag tggagccggt agataccttt gcgcttgaac aaatggctcg cctggtggaa
    14161 gcgcgtctgg ctgatttccg tattaaagcc gatgttgtca attactctcc ggggccggtt
    14221 atcactcgct ttgaattgaa cctggcaccg ggcgtaaaag cggcgcgcat ttctaacttg
    14281 tcacgggacc ttgcccgttc actttcgacg gtggcggtgc gtgtcgttga agttattcct
    14341 ggcaaaccct atgtaggtct ggagttaccg aataaaaaac gacaaaccgt ttatctgcgc
    14401 gaagttttgg ataacgccaa attccgcgat aatccgtcgc cattaaccgt ggtgctgggt
    14461 aaagatatcg ccggtgagcc ggtggttgcc gatctggcga aaatgccgca cttgttggtt
    14521 gcggggacta ccggttccgg taaatctgtc ggtgtgaacg cgatgatcct gagcatgctt
    14581 tataaagcac agccagaaga tgtgcgtttc atcatgatcg acccgaaaat gctggagctt
    14641 tcggtttatg aaggcattcc gcatctgtta acggaagtcg ttactgatat gaaagatgcc
    14701 gccaacgcgc tgcgctggtg tgttaacgag atggagcgtc gttataaact gatgtctgcg
    14761 ctgggtgtgc gtaatctggc gggttataac gaaaaaattg ctgaagccga tcgcatgatg
    14821 cgtccgattc cagacccgta ctggaagccg ggtgacagta tggatgccca gcatccggtg
    14881 ctgaaaaaag aaccatacat tgtggtgttg gttgacgaat ttgccgacct gatgatgacg
    14941 gtaggtaaaa aagtggaaga gctgatagca cgtctggcgc aaaaagcccg tgccgcgggt
    15001 atccacctcg tactggcaac tcagcgtcca tcggttgatg ttattactgg tctgattaaa
    15061 gcgaatattc cgacccgtat cgcctttacc gtatccagta agattgactc acgtaccatt
    15121 cttgatcagg ctggcgcgga atcactgctg gggatggggg atatgctcta ctctgggccg
    15181 aactccacgt tgccggtacg tgtccatggt gcttttgttc gcgatcagga agttcatgcc
    15241 gtggtgcagg actggaaagc gcgtggtcgc ccacagtatg ttgatggcat cacttccgac
    15301 agtgaaagcg aaggtggcgc gggcggtttc gatggcgctg aagaactgga tccgttgttc
    15361 gatcaggcgg tgcagtttgt cactgaaaaa cgcaaagcgt caatttctgg cgtacagcgt
    15421 cagttccgca ttggttataa ccgtgcagcg cgtattatcg aacagatgga agcgcagggg
    15481 attgtcagcg aacaggggca caacggtaat cgtgaagtgc tggctccacc gccgtttgac
    15541 taattaatgc attgccggat aaggcgcggt agcgtcgcat ccggcactct atcaactgaa
    15601 aattcagtat tttcttcttt cctcaagctg attattagcc tggaatagag agtagaggga
    15661 actcccgatc gggagtgacg taatttgagg aataatgatg aaaaaaattg ccatcacctg
    15721 tgcattactc tcaagcttag tagcaagcag cgtttgggct gatgccgcaa gcgatctgaa
    15781 aagccgcctg gataaagtca gcagcttcca cgccagcttc acacaaaaag tgactgacgg
    15841 tagcggcgcg gcggtgcagg aaggtcaggg cgatctgtgg gtgaaacgtc caaacttatt
    15901 caactggcat atgacacaac ctgatgaaag cattctggtt tctgacggta aaacactgtg
    15961 gttctataac ccgttcgttg agcaagctac ggcaacttgg ctgaaagatg ccaccggtaa
    16021 tacgccgttt atgctgattg cccgcaacca gtccagcgac tggcagcagt acaatatcaa
    16081 acagaatggc gatgactttg tcctgacgcc gaaagccagc aatggcaatc tgaagcagtt
    16141 caccattaac gtgggacgtg atggcacaat ccatcagttt agcgcggtgg agcaggacga
    16201 tcagcgcagc agttatcaac tgaaatccca gcaaaatggg gctgtggatg cagcgaaatt
    16261 taccttcacc ccgccgcaag gcgtcacggt agatgatcaa cgtaagtaga ggcacctgag
    16321 tgagcaatct gtcgctcgat ttttcggata atacttttca acctctggcc gcgcgtatgc
    16381 ggccagaaaa tttagcacag tatatcggcc agcaacattt gctggctgcg gggaagccgt
    16441 tgccgcgcgc tatcgaagcc gggcatctgc attctatgat cctctggggg ccaccgggta
    16501 ccggcaaaac aaccctcgct gaagtgattg cccgctatgc gaacgctgat gtggaacgta
    16561 tttctgccgt cacctctggc gtgaaagaga ttcgcgaggc gatcgagcgc gcccggcaaa
    16621 accgcaatgc aggtcgccgc actattcttt ttgttgacga agtgcaccga ttcaacaaaa
    16681 gccagcagga tgcatttctg ccacatattg aagacggcac catcactttt attggcgcaa
    16741 ccactgaaaa cccgtcgttt gagcttaatt cggcactgct ttcccgtgcc cgtgtctatc
    16801 tgttgaaatc cctgggtaca gaggatattg agcaagtact aactcaggcg atggaagaca
    16861 agacccgtgg ttatggtggt caggatattg ttctgccaga tgaaacacga cgcgccattg
    16921 ctgaactggt gaatggcgac gcgcgccggg cgttaaatac gctggaaatg atggcggata
    16981 tggccgaagt caatgatagc ggtaagcggg tcctgaagcc tgaattattg accgaaatcg
    17041 ccggtgagcg aagcgcccgc tttgataaca aaggcgatcg cttttacgat ctcatttccg
    17101 cactgcataa gtcggtacgt ggtagcgcac ccgatgcggc gctctactgg tatgcgcgaa
    17161 ttattaccgc tggtggcgat ccgttatatg tcgcgcgtcg ttgcctggca attgcttccg
    17221 aagatgtcgg caatgccgac ccacgggcga tgcaggtggc aattgcggcc tgggattgct
    17281 ttactcgcgt tggcccggcg gaaggtgaac gcgccattgc tcaggcgatt gtttacctgg
    17341 cctgcgcgcc aaaaagcaac gctgtctaca ctgcgtttaa agctgcgctg gcggatgctc
    17401 gcgaacgccc ggattatgac gtgccggttc atttgcgtaa tgcgccgacg aaattaatga
    17461 aggaaatggg ctacgggcag gaatatcgtt acgctcatga tgaagcaaac gcttatgctg
    17521 ccggtgaggt ttacttcccg ccggaaatag cacaaacacg ctattatttc ccgacaaaca
    17581 ggggccttga aggcaagatt ggcgaaaagc tcgcctggct ggctgaacag gatcaaaata
    17641 gccccataaa acgctaccgt taatgttatc gttgcggtaa tgttgttact gtatccctgt
    17701 ggtcgcaggc tgtggccaca tctcccattt aattcgataa gcacaggata agcatgctcg
    17761 atcccaatct gctgcgtaat gagccagacg cagtcgctga aaaactggca cgccggggct
    17821 ttaagctgga tgtagataag ctgggcgctc ttgaagagcg tcgtaaagta ttgcaggtca
    17881 aaacggaaaa cctgcaagcg gagcgtaact cccgatcgaa atccattggc caggcgaaag
    17941 cgcgcgggga agatatcgag cctttacgtc tggaagtgaa caaactgggc gaagagctgg
    18001 atgcagcaaa agccgagctg gatgctttac aggctgaaat tcgcgatatc gcgctgacca
    18061 tccctaacct gcctgcagat gaagtgccgg taggtaaaga cgaaaatgac aacgttgaag
    18121 tcagccgctg gggtaccccg cgtgagtttg actttgaagt tcgtgaccac gtgacgctgg
    18181 gtgaaatgca ctctggcctc gactttgcag ctgcagttaa gctgactggt tcccgctttg
    18241 tggtaatgaa agggcagatt gctcgcatgc accgcgcact gtcgcagttt atgctggatc
    18301 tgcataccga acagcatggc tacagtgaga actatgttcc gtacctggtt aaccaggaca
    18361 cgctgtacgg tacgggtcag ctgccgaaat ttgctggcga tctgttccat actcgtccgc
    18421 tggaagaaga agcagacacc agtaactatg cgctgatccc aacggcagaa gttccgctga
    18481 ctaacctggt gcgcggtgaa atcatcgatg aagatgatct gccaattaag atgaccgccc
    18541 acaccccatg cttccgttct gaagccggtt catatggtcg tgacacccgt ggtctgatcc
    18601 gtatgcacca gttcgacaaa gttgaaatgg tgcagatcgt gcgcccggaa gactcaatgg
    18661 cggcgctgga agagatgact ggtcatgcag aaaaagtcct gcagttgctg ggcctgccgt
    18721 accgtaaaat catcctttgc actggcgaca tgggctttgg cgcttgcaaa acttacgacc
    18781 tggaagtatg gatcccggca cagaacacct accgtgagat ctcttcctgc tccaacgttt
    18841 gggatttcca ggcacgtcgt atgcaggcac gttgccgcag caagtcggac aagaaaaccc
    18901 gtctggttca taccctgaac ggttctggtc tggctgttgg tcgtacgctg gttgcagtaa
    18961 tggaaaacta tcagcaggct gatggtcgta ttgaagtacc agaagttctg cgtccgtata
    19021 tgaacggact ggaatatatt ggctaatacc caatttttct gaatctaaaa agcgcctgcg
    19081 ggcgcttttt ttgtctccct ttgataccga acaataatta ctcttcactt acacgtaata
    19141 ctactttcga gtgaaaatct acctatctct ttgattttca aattattcga tgtatacaag
    19201 cctatatagc gaactgctat agaaataatt acacaatacg gtttgttact ggaatcaatc
    19261 gtgagcaagc ttgagtgagc cattatgaaa acgaaaatcc ctgatgcggt attggctgct
    19321 gaggtgagtc gccgtggttt ggtaaaaacg acagcgatcg gcggcctggc aatggccagc
    19381 agcgcattaa cattaccttt tagtcggatt gcgcacgctg tcgatagcgc cattccaaca
    19441 aaatcagacg aaaaggttat ctggagcgcc tgtacagtta actgtggtag tcgctgcccg
    19501 ctacgtatgc acgtcgtgga cggtgaaatc aaatatgtcg aaacggacaa taccggcgat
    19561 gacaattacg acggcctgca ccaggttcgc gcctgcctgc gtgggcgttc catgcgtcgc
    19621 cgtgtctaca atccggaccg cctgaaatat ccgatgaaac gagtcggggc gcgcggtgaa
    19681 ggcaaattcg agcgcattag ctgggaagaa gcctacgaca tcatcgcgac caatatgcag
    19741 cgcctgatca aagagtacgg caacgagtct atctatctga actatggcac cggtacgctg
    19801 ggcggcacca tgacccgctc ctggccgccg ggaaataccc tggtcgcgcg gctgatgaac
    19861 tgctgcggcg gctatctgaa ccattacggc gactactcct ccgcgcaaat tgcggaaggt
    19921 ttgaactata cctacggcgg ctgggcagat ggcaacagcc cgtcggatat cgaaaacagt
    19981 aagctggtag tgctgtttgg taataaccct ggcgaaacgc gaatgagtgg cggtggggtg
    20041 acttactatc ttgaacaggc acgccagaaa tctaatgccc gcatgatcat catcgatccg
    20101 cgctataccg acaccggtgc cgggcgcgaa gatgagtgga tccctattcg tccgggaaca
    20161 gatgccgcac tggttaacgg tctggcgtac gtcatgatca ctgaaaacct ggtggatcag
    20221 gcattcctcg ataaatattg cgttggctac gatgagaaaa ccctgccagc cagtgcgccg
    20281 aaaaatggcc actataaagc ttatattctg ggtgaagggc cagatggcgt ggctaaaaca
    20341 ccggaatggg cctcacaaat caccggtgtt ccggcagaca aaatcatcaa actggctcgt
    20401 gaaatcggca gtaccaaacc ggcgtttatc agccagggat ggggcccgca gcgtcacgct
    20461 aacggtgaaa tcgcaacccg tgctatctcg atgctggcga ttctgaccgg taacgttggt
    20521 attaatggag ggaacagcgg cgcgcgtgaa ggttcataca gcttaccgtt tgtccgtatg
    20581 ccgaccttgg aaaacccgat ccagaccagc atttcgatgt ttatgtggac cgatgccatt
    20641 gaacgtggcc cggaaatgac ggcgctgcgt gatggtgtac gcgggaaaga taagctggat
    20701 gtgccgatca aaatgatctg gaactatgcc ggtaactgcc tggttaacca gcattctgaa
    20761 atcaaccgta cccatgaaat ccttcaggat gataagaagt gcgagctgat tgtggttatc
    20821 gactgccaca tgacctcatc ggcgaaatat gctgacatcc tgctgcctga ctgcaccgct
    20881 tccgaacaga tggactttgc gctggatgca tcctgcggga atatgtctta cgtgattttc
    20941 aacgatcagg tgattaaacc gcgctttgaa tgtaagacca tctatgaaat gaccagcgaa
    21001 ctggcaaaac gtcttggcgt tgagcaacag tttactgaag gccgtaccca ggaagagtgg
    21061 atgcggcatc tgtatgccca gtcgcgggaa gcgattcctg aactgccaac gtttgaagag
    21121 ttccgcaagc aggggatctt taaaaagcgc gacccacaag ggcatcacgt tgcttataaa
    21181 gccttccgtg aagatccgca ggcaaaccca ctgactacgc catcgggcaa aattgagatt
    21241 tattcgcagg cgctggctga cattgccgct acctgggaat tgccagaagg cgatgtgatc
    21301 gatccactgc cgatctacac gccgggcttt gaaagttatc aggatccgct gaacaaacag
    21361 tatccgctgc agcttacggg tttccactat aaatctcgcg ttcactcaac ttacggcaac
    21421 gttgatgtgc tgaaagcagc ttgccgtcag gaaatgtgga tcaacccgct tgatgcgcaa
    21481 aaacgcggta tcaacaacgg cgataaagtc aggatcttta acgatcgtgg tgaagttcat
    21541 attgaggcga aagtgacgcc acgaatgatg ccaggtgtgg tcgcactggg tgaaggtgcc
    21601 tggtatgacc cggatgcaaa acgtgtcgat aagggtggtt gtattaacgt actgaccact
    21661 caacgtccgt ctcctctcgc taaggggaat ccgtcacata caaaccttgt tcaggttgaa
    21721 aaggtgtaag gagtaaccga tgacaaccca gtatggattt tttattgatt ccagccgttg
    21781 caccggttgc aaaacctgcg agctggcttg taaagactac aaagatttga cgccagaagt
    21841 cagcttccgc cgcatttatg aatatgctgg cggcgactgg caggaagata acggtgtctg
    21901 gcaccagaac gtgtttgcct actatctgtc gatttcgtgc aaccactgcg aagatccggc
    21961 ttgtactaaa gtctgcccga gcggtgcgat gcataaacgt gaagatggtt ttgttgtggt
    22021 cgatgaagat gtgtgcattg gctgccgcta ctgccatatg gcttgcccgt atggcgcacc
    22081 gcaatataac gaaacgaaag gccatatgac caaatgcgat ggttgttatg accgtgttgc
    22141 ggagggtaaa aagccgatct gtgttgaatc ttgtccgctg cgggcgctgg atttcggccc
    22201 tatcgacgag ctgcgtaaaa aacatggcga tctggcggcc gttgcgccgt tgccgcgagc
    22261 tcactttacc aaaccgaata ttgtgatcaa acccaatgcc aatagccgcc cgaccgggga
    22321 taccactggc tatctggcaa acccgaagga ggtgtgagat gggaagtgga tggcatgaat
    22381 ggccgctgat gatcttcacg gtcttcgggc aatgtgtggc gggtggtttt atcgttctgg
    22441 ctttggcgct gctcaaaggc gacctgcgag cagaagccca gcagcgtgtt atcgcctgca
    22501 tgtttggctt atgggtgctg atgggcattg gctttatcgc ttctatgctc catcttggtt
    22561 caccgatgcg cgcttttaac tcgctcaacc gggtaggggc ttcagcactc agtaacgaaa
    22621 tcgccagcgg ttcgatcttt tttgccgtag gcggcatcgg ctggttgctg gcaatgctga
    22681 aaaagctgtc accggcattg cgtacgcttt ggctggtggt gacgatggtt cttggcgtcg
    22741 tctttgtctg gatgatggtg cgtgtgtata acagcattga taccgttccg acctggtaca
    22801 gcatctggac gccgatgggc ttcttcctga cgatgttcat gggcggcccg ttactggggt
    22861 acctgttgct gagtctggct ggcgtcgata gctgggcgat gcgtctgctg ccagccattt
    22921 ccgtactggc actggtggtg agtggcgtgg tgtcagtgat gcagggcgca gagctggcga
    22981 ctattcatag ttctgtgcag caggccgcag cgctggtgcc ggactatggt gcgctgatgt
    23041 cctggcggat cgtgcttttg gccgttgccc tgtgcttgtg gattgcacca cagctaaaag
    23101 gttatcagcc tgcggttccg ctactatccg tctcattcat tctgcttctg gcaggggaat
    23161 taatcggtcg cggcgtattc tatggtttgc atatgaccgt ggggatggcc gtcgcaagct
    23221 aatcagtaac aaccggggtt tcggccccgg ttctctttta tttctgcttc gttaacgtgt
    23281 cataactggt catcaaatta cgataatcag gaatatggtt agagaacaac gtcgccagtc
    23341 cttcaatatc attacgccag tcacggtgca gctcgcaagc cacgccgaac catgtcatca
    23401 attgcgcacc tgcttgcgac atgcgatccc atgccgaatg ccgggtaatt tcattaaagg
    23461 tccctgacgc gtcagtcaca acaaacacat caaacccttc ttcaatggct gaaagcgccg
    23521 ggaatgccac gcaaacttcg gttaccacac cggcaataat taactgtttt ttacctgtcg
    23581 ctttgactgc ttttacaaaa tcttcgttat cccaggcgtt aatattgccc gggcgagcaa
    23641 tgtaaggtgc atcggggaat tgtgctttca gttctggaac taatgggccg ttaggaccgg
    23701 tttcaaaact ggtggtaaga atagttggta agttgaaata cttagccagg tcacccagcg
    23761 ccagcacgtt atttttaaac ttatcgggtt cgatatcccg tacaagggaa agtaaaccag
    23821 cctggtgatc aacaagcaaa acagcagcat catttttatc aagacgaaca tacggtttgg
    23881 tcatcctctt ctcctttcga gagtcagtgg tacgcctgaa atcggtcaga cgtgatgcat
    23941 tgctctgaaa gcatagacgg gaaatatgag tttgctgtga ccatgaaatt tttcgactga
    24001 acgaagcgta tgaaatttgt gttagatcaa taaaaacaat cagatgagat taatcatctc
    24061 ttcagtatta gcgttatgaa tgagtaatat aaatcgataa taccgccgtt gcgatatgcg
    24121 cgtattttca cccattgaca atgtttttgg cggtggcatg atgcgcatga aatttgaact
    24181 tcctcacggt tttaattcat gtccacgtat acccggcctg tcatgctttt gctgtctggc
    24241 ctgcttttgt tgactctggc gatcgcggtg ttaaatacac tcgtgccgct ttggctcgcc
    24301 caggaacaca tgtccacatg gcaggtaggc gttgtcagct catcctattt taccggcaac
    24361 cttgtcggta cattgctgac agggtatgtc attaaacgca ttggttttaa ccgcagctat
    24421 tatctggcct ccttcatttt tgccgctggc tgtgccggcc ttggcctgat gattggattc
    24481 tggagttggt tggcgtggcg ttttgtcgcg ggcgtcggct gtgccatgat ttgggtagtc
    24541 gttgagagcg cgctgatgtg cagtgggacg tcacgtaacc gtgggcgttt gcttgctgca
    24601 tacatgatgg tttattacgt gggaacgttt ttaggccagt tactggtcag caaggtctca
    24661 accgagctga tgagcgtatt gccgtgggtt acaggtttga cattggcagg gatcttaccg
    24721 ctgttgttta cgcgtgtcct gaatcagcag gctgaaaacc atgattcgac gtcaattacg
    24781 gcaatgctaa aactccgtca ggcgcggctt ggcgtgaatg gctgcattat ctcaggaatc
    24841 gttctgggat ctctatatgg cctgatgcct ctgtacctca atcacaaagg ggtgagcaat
    24901 gccagtattg gtttctggat ggcggtactg gtcagtgcgg gtatcctcgg acaatggccg
    24961 attggacgtc tggcggataa gtttggtcga ctgctggttt tgcgtgttca ggtctttgtc
    25021 gtcattctcg gcagtattgc gatgcttagc caggcggcga tggccccagc gttattcatc
    25081 ctcggtgccg ctggctttac gctatatccg gtggcgatgg catgggcttg cgagaaagtt
    25141 gaacatcatc aactggtggc gatgaaccaa gccttactgt tgagctatac tgtgggaagt
    25201 ctgcttggcc cgtcatttac cgctatgcta atgcagaatt tctccgataa tttattgttt
    25261 atcatgatcg ccagcgtatc gtttatctat ttgctgatgc tgctgcgcaa cgccggtcat
    25321 acgccgaaac ccgttgctca cgtgtaaatg aattcaagca gagtgtgaac ttactgtttc
    25381 acactctgct tttttgtttc ttctatctga cttgctttat tccaaatttt attcgtttaa
    25441 aaataaaatg tgtagcaggt tataattttg catttcgcta tttccgcact tcttatttgc
    25501 cgcgcataat ccctcgtttt acagatgccc ctataatttt ggcgaaggat ttgtctatgg
    25561 ctgggaatgt tcaggaaaaa cagctgcgat ggtacaacat tgcgctgatg tcttttatca
    25621 ctgtctgggg ttttggcaac gttgttaata actacgccaa ccaggggctg gtggttgttt
    25681 tttcatgggt gtttatcttt gcgctctatt tcacacctta tgcgctaatt gttggtcagt
    25741 taggctcgac cttcaaagat gggaagggcg gggtcagtac ctggattaaa cacacgatgg
    25801 gacccggact ggcttatctc gccgcgtgga cctactgggt ggtgcatatt ccctatctgg
    25861 cacaaaaacc ctaggcaatt ctgattgcgc tcggttgggc gatgaaaggc gacggttcgc
    25921 taatcaaaga atattcagtc gtagcgttac aggggttaac gctggtgctg tttatcttct
    25981 ttatgtgggt tgcttcacgc ggtatgaaat cgctgaaaat cgtcggttct gtggcaggga
    26041 ttgctatgtt tgttatgtcg ctcctgtatg tggcgatggc ggtaaccgcg cctgcaatta
    26101 ctgaagtgca tattgcgacc acaaacatta cctgggaaac gttcattcct catatcgact
    26161 ttacctacat taccactatt tcaatgctgg ttttcgcggt tggcggagca gagaagattt
    26221 ctccttacgt taatcaaacg cgcaacccag gaaaagaatt tccaaaaggg atgttatgcc
    26281 tggcggtgat ggttgcggtt tgtgccattc tgggctcgct ggcgatgggg atgatgtttg
    26341 attcgcgtaa tatcccggat gacttaatga ccaacggtca gtattacgcc tttcagaagc
    26401 tgggcgagta ttacaacatg ggtaatactt taatggtgat ttacgccatt gcgaataccc
    26461 tgggacaagt agcggcgctg gtattctcga ttgatgcccc gcttaaagtg ctattaggtg
    26521 atgctgacag caaatatatt ccagccagtt tatgtcgtac caacgcttct ggtacgcccg
    26581 ttaatggcta ttttctgacc ctggtactgg tggcgattct gattatgctg ccgactctcg
    26641 gcattggtga tatgaacaat ctctataaat ggctgttgaa ccttaattcg gtagtgatgc
    26701 cgctgcgtta tttatgggta tttgttgcat ttattgcagt cgttcgcctg gcgcagaaat
    26761 ataaaccaga atatgtcttt attcgtaata agcctctggc aatgaccgtc gggatttggt
    26821 gttttgcctt taccgccttt gcctgtttga cggggatctt cccgaaaatg gaagccttca
    26881 ctgcagagtg gaccttccag ttggcgctga atgttgcaac gccgtttgtg ctggtaggat
    26941 taggactgat attcccgctg ctggcgcgta aagcgaatag taaataatta ttgtggtcgg
    27001 tgacgctgac tctggcagat ctgacccgtt tttacataat cgagaaatgc gcgcaagccg
    27061 caggacatat actggcggtt tgggtaatag atctggaagc ctggacgctc tgtgatccag
    27121 tcttccagta cacattctaa acgaccggta tcaaggtact ccttgatctg ttcatagagc
    27181 agatacccga taccagcacc catcaaaacg gcatccagtt cagcatcaac atca
//
LOCUS       DAEEKQ010000041        27104 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.041, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000041 DAEEKQ010000000
VERSION     DAEEKQ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 27104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.041"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..921
                     /locus_tag="KLK57_001664"
     CDS             <1..921
                     /locus_tag="KLK57_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0892050.1"
                     /translation="SATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLN
                     DSLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVN
                     IGANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLT
                     APQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQM
                     RIDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTV
                     NMRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="KLK57_001665"
     CDS             complement(964..1824)
                     /gene="rsmI"
                     /locus_tag="KLK57_001665"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HBE0892051.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            1888..3924
                     /gene="lpoA"
                     /locus_tag="KLK57_001666"
     CDS             1888..3924
                     /gene="lpoA"
                     /locus_tag="KLK57_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HBE0892052.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKKQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGTPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGAML
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPVS"
     gene            3882..4277
                     /locus_tag="KLK57_001667"
     CDS             3882..4277
                     /locus_tag="KLK57_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HBE0892053.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            4297..4887
                     /gene="diaA"
                     /locus_tag="KLK57_001668"
     CDS             4297..4887
                     /gene="diaA"
                     /locus_tag="KLK57_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HBE0892054.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            4897..5472
                     /gene="yraP"
                     /locus_tag="KLK57_001669"
     CDS             4897..5472
                     /gene="yraP"
                     /locus_tag="KLK57_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HBE0892055.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(5586..6626)
                     /locus_tag="KLK57_001670"
     CDS             complement(5586..6626)
                     /locus_tag="KLK57_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBE0892056.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(6699..7334)
                     /locus_tag="KLK57_001671"
     CDS             complement(6699..7334)
                     /locus_tag="KLK57_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HBE0892057.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            7462..7980
                     /gene="yhbO"
                     /locus_tag="KLK57_001672"
     CDS             7462..7980
                     /gene="yhbO"
                     /locus_tag="KLK57_001672"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HBE0892058.1"
                     /translation="MSKKIAVLISDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(7960..8403)
                     /locus_tag="KLK57_001673"
     CDS             complement(7960..8403)
                     /locus_tag="KLK57_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HBE0892059.1"
                     /translation="METLITISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARQAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            8454..8756
                     /gene="yhbQ"
                     /locus_tag="KLK57_001674"
     CDS             8454..8756
                     /gene="yhbQ"
                     /locus_tag="KLK57_001674"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HBE0892060.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(8743..9246)
                     /locus_tag="KLK57_001675"
     CDS             complement(8743..9246)
                     /locus_tag="KLK57_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE0892061.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(9240..9764)
                     /locus_tag="KLK57_001676"
     CDS             complement(9240..9764)
                     /locus_tag="KLK57_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HBE0892062.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            9973..10968
                     /locus_tag="KLK57_001677"
     CDS             9973..10968
                     /locus_tag="KLK57_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBE0892063.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            10977..11855
                     /locus_tag="KLK57_001678"
     CDS             10977..11855
                     /locus_tag="KLK57_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HBE0892064.1"
                     /translation="MKYSLGPVLWYWPKEALEEFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELTSMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12061..13068
                     /locus_tag="KLK57_001679"
     CDS             12061..13068
                     /locus_tag="KLK57_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="luciferase-like monooxygenase"
                     /protein_id="HBE0892065.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="KLK57_001680"
     CDS             complement(13186..14430)
                     /gene="mtr"
                     /locus_tag="KLK57_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HBE0892066.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="KLK57_001681"
     CDS             complement(14584..16473)
                     /gene="deaD"
                     /locus_tag="KLK57_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HBE0892067.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="KLK57_001682"
     CDS             complement(16466..16546)
                     /gene="yrbN"
                     /locus_tag="KLK57_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HBE0892068.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="KLK57_001683"
     CDS             complement(16653..17537)
                     /gene="nlpI"
                     /locus_tag="KLK57_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HBE0892069.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="KLK57_001684"
     CDS             complement(17646..19781)
                     /gene="pnp"
                     /locus_tag="KLK57_001684"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HBE0892070.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="KLK57_001685"
     CDS             complement(20028..20297)
                     /gene="rpsO"
                     /locus_tag="KLK57_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HBE0892071.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(20446..21390)
                     /gene="truB"
                     /locus_tag="KLK57_001686"
     CDS             complement(20446..21390)
                     /gene="truB"
                     /locus_tag="KLK57_001686"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HBE0892072.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="KLK57_001687"
     CDS             complement(21390..21791)
                     /gene="rbfA"
                     /locus_tag="KLK57_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HBE0892073.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(21955..24627)
                     /gene="infB"
                     /locus_tag="KLK57_001688"
     CDS             complement(21955..24627)
                     /gene="infB"
                     /locus_tag="KLK57_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HBE0892074.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEAHRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="KLK57_001689"
     CDS             complement(24652..26139)
                     /gene="nusA"
                     /locus_tag="KLK57_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HBE0892075.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="KLK57_001690"
     CDS             complement(26167..26619)
                     /gene="rimP"
                     /locus_tag="KLK57_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HBE0892076.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(26826..26902)
                     /locus_tag="KLK57_001691"
     tRNA            complement(26826..26902)
                     /locus_tag="KLK57_001691"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(26866..26868),aa:Met,seq:cat)
BASE COUNT         6419 a         7516 c         6812 g         6357 t
ORIGIN      
        1 tctgcaacgc taagtagcac taactggcaa tacgcctgtt cctgctctgc gggtaaggca
       61 gttaaacttg tctatatggt cagccccgta cttaccacca ctggacatca gacaggatat
      121 tacaaactca atgacagcct ggatattaaa accacattac aggcaaacga cattccagga
      181 ctcacaaccg accaggttgt ctctgttaac acccgattca cacagataaa aagcagcacc
      241 gtatattctg ctgcaaccca aacgggtgtt tgccagggtg acacgtctcg ttatggaccc
      301 gttaatattg gtgcaaatac cacctttacc ctgtatgtca ccaagccatt tctcggctcg
      361 atgaccattc cgaaaacgga tattgccgtc attaaaggcg cgtgggtcga tggaatggga
      421 agcccgtcta caggtgactt ccatgattta gtcaagttat cgattcaggg aaatctcacc
      481 gccccacagt cgtgcaaaat taatcagggc gatgttatta aggttaattt tggattcatc
      541 aatggtcaga agtttaccac ccgcaatgcc atgccagacg gttttactcc agtagacttt
      601 gatatcactt atgactgtgg tgatacttca aagattaaaa actcgttgca aatgcgcatc
      661 gacggtacaa ctggggtagt agaccagtac aacctggtcg ccaggcgaag aagttcagac
      721 aatgtgcccg atgtcggtat tcgtattgaa aatctcggcg gcggagttgc aaatattcct
      781 tttcagaacg gtatccttcc cgttgatcct tccgggcatg gcaccgtcaa tatgcgcgcc
      841 tggccagtta atctggtcgg tggtgagctg gaaacaggaa aatttcaggg caccgccacc
      901 attaccgtca tggtgcggta aaagaagacg ttgcgttttg attaacgcaa cgcttcggca
      961 ctgttacccc tgctgctcca gcgcatactt atacagcgca tttttcttca cgccatgaat
     1021 ttctgcggcc agcgccgccg cttttttcag cggcagttct gcctgtagca gcgccagcgt
     1081 gcgcagggca tcggcgggta agtcttcttc ctgtgcttta tgaccttcga caatcagcac
     1141 catttcgcct ttgcgacggt tttcatcttc ctttacccac gccagcagct cgccaacggg
     1201 cgcgccgtga atggtttccc aggttttggt cagctcacgc gccagaacca cgtagcggga
     1261 ttcgcctaat accgcaacga tatcttccag gctatctaac agacggtggg tagattcata
     1321 aaaaatcagc gtgcgcggct ccgcttcaat ggcttttagc gcatcacggc ggccttttga
     1381 tttggcaggt aaaaagcctt cgtaacagaa acggtcagag ggtaaacccg cggcgcttaa
     1441 cgcagtgata gcagcacacg gtccgggtag cggcaccaca cgaatccccg cttcacggca
     1501 ggtacgcacc agatggtagc caggatcgtt aattagcggc gttccggcat cggaaaccag
     1561 cgcaatgttt tgcccctctt gcagcttcgc cagcagcgtt tcggcttttt gttgttcgtt
     1621 atggtcgtgc agcgcaaaca accgggcatt aatcccaaaa tgttgcagca ataaaccggt
     1681 gtgacgagta tcctcggcgg caatcagatc aacggcctgt aatacctcta acgcacgctg
     1741 ggtgatatcc gccagattgc cgattggcgt cggtacaatg taaagctggc cctgagaatt
     1801 atccgccgat tggtgttgtt tcattgtgtc gtccgtattg ccgatttaat attgagcatt
     1861 gcgtaaaaaa atatcactgg atacattatg gtaccctcaa cattttctcg tttgaaagcc
     1921 gcgcgttgtc tgcctgttgt tctggcagcc ctgattttcg ccggttgtgg cacccatact
     1981 cccgatcagt ccactgctta tatgcagggc acggcgcagg ctgattctgc cttttatctt
     2041 cagcagatgc agcaaagctc tgatgatacc aggatcaact ggcaattact cgccattcgt
     2101 gcactggtga aagaaggtaa aaccgggcag gcggttgagt tgtttaacca actaccgcaa
     2161 gaactgaacg attctcagcg tcgcgagaaa acactgctgg cggtagagat taaactggcg
     2221 cagaaagatt ttgctggcgc gcaaaacttg ctggcgaaaa tcacacctgc cgatttagaa
     2281 caaaaccagc aagcgcgtta ctggcaggca aaaatcgatg ccagccaggg gcgtccttcc
     2341 attgatttac tgcgcgcgtt aattgctcag gaaccgctgc tcggcgcgaa agaaaaaaag
     2401 cagaatattg atgccacctg gcaggcgctc tcctccatga ctcaggaaca ggcgaatacg
     2461 ctggtgatca acgccgacga aaatattctg caaggctggc tggatctgca gcgcgtctgg
     2521 tttgataacc gtaacgatcc cgacatgatg aaagccggga tcgccgactg gcaaaaacgt
     2581 tatccgaaca atccgggcgc gaaaatgctg ccaacgcagt tggttaacgt aaaagcgttt
     2641 aaaccagcct cgaccaacaa aatcgccctg ctgttgccgc tgaatggcca ggcagcggta
     2701 tttggtcgca ccattcagca aggctttgaa gcggcgaaaa atatcggcac tcagccagtg
     2761 gcggctcagg tagctgccgc acctgccgca gacgtagctg aacaacctca gccgcaaacc
     2821 gtggatggcg ttgccagccc ggcacaagcc tcggttagcg atctgaccgg tgatcagcct
     2881 gcggcccagc cggtgcctgt aagcgccccg gcgacaagca ccgcagcggt aagcgcaccc
     2941 gcaaatccat ccgcagagct gaaaatctac gatacctcat cacaaccact tagccagatc
     3001 ttaagccagg ttcagcagga tggcgcgagt attgtggttg gtccgttgct gaaaaataac
     3061 gttgaagagt tgctgaagag caacaccccg ctgaacgtgc tggcactgaa ccagccagag
     3121 aatatcgaaa accgcgtcaa tatttgttac ttcgcgcttt cacctgaaga cgaagcgcgc
     3181 gatgcagcgc gtcatattcg tgaccagggt aaacaagcgc cgctggtgct gatcccacgc
     3241 agttcattgg gcgatcgcgt agccaatgcg tttgcgcaag agtggcagaa actgggcggc
     3301 ggcaccgttc tgcaacaaaa atttggttcc accagcgaat tacgcgcggg tgttaacggc
     3361 ggttctggta tcgctttaac gggtaccccg attactccca gagcgacaac cgactccggc
     3421 atgacgacca acaatccaac gctgcaaacc acgccaaccg atgaccagtt caccaataat
     3481 ggcggtcgtg tcgatgcggt gtacattgtg gcaacgccgg gtgaaatcgc ttttatcaaa
     3541 ccgatgatcg ccatgcgtaa cggtagccag agcggtgcaa tgctgtacgc cagctcccgc
     3601 agcgcacaag gcaccgcagg cccggatttc cgtctggaga tggaaggttt gcagtacagc
     3661 gaaatcccga tgctggcggg cggtaatctg ccgttaatgc agcaggcact cagcgcggtg
     3721 aataacgatt attcactggc tcgcatgtat gcgatgggcg tcgatgcctg gtcgctggca
     3781 aatcatttct cacaaatgcg ccaggttcag ggttttgaaa tcaacggtaa taccggaagc
     3841 ctgacggcaa acccggattg cgtgattaac aggaagttat catggctaca gtaccaacaa
     3901 ggtcaggtag tccccgtcag ttaaccacca aacagaccgg cgatgcgtgg gaagcacaag
     3961 cgcgtcgctg gctggaaggc aaaggactgc ggtttatcgc cgctaacgtg aacgagcgtg
     4021 gcggcgagat cgatctgata atgcgtgaag gccggaccac cgtttttgtc gaggtacgct
     4081 atcgccgctc tgcgctttat ggcggcgcgg cagccagtgt gacccgcagc aaacaacaca
     4141 aattattaca gactgcccgc ttgtggctcg cgcgtcataa tgggagtttt gatactgtgg
     4201 attgccggtt cgatgtggta gccttcaccg ggaatgaggt tgagtggatt aaggatgcct
     4261 ttaatgacca ctcataatta aggtttaagg attagcgtgc aagaaagaat taaagcttgc
     4321 ttcactgaaa gcattcaaac tcaaattgcg gcggcagagg cgcttccgga tgccatctcc
     4381 cgtgcagcca tgacgctggt tcagtctctg ctcaatggca acaaaatcct ctgttgtggt
     4441 aatggaactt ccgctgccaa tgcacagcat tttgctgcca gcatgatcaa ccgtttcgaa
     4501 acggagcggc ccagcttacc tgccattgca ctaaatactg ataatgttgt cttaacggcg
     4561 attgccaacg atcgcttaca tgatgaagtg tatgcaaaac aggtgcgggc gctgggtcat
     4621 gcgggagatg tattgttagc catttccacc cgtggcaaca gccgcgatat tgttaaagca
     4681 gttgaagccg ccgttacgcg tgatatgacc attgtggcat tgaccggcta tgacggcggc
     4741 gagcttgcag gtttgttagg gccacaggat gtggagatcc gcattccttc gcatcgtagt
     4801 gctcgcattc aggaaatgca tatgctgacg gtaaattgcc tgtgcgatct gatcgataac
     4861 acgcttttcc ctcaccagga tgattaagga gaatacatga aggcattatc gccaatcgca
     4921 gtccttattt ccgcgctgct gttgcaaggt tgtgttgccg ctgccgtagt gggtaccgct
     4981 gctgtgggta ccaaagccgc aactgaccca cgcagtgtcg gcacccaggt ggacgatggt
     5041 accctggaag tgcgcgtgaa cagcgcattg tcgaaagacg aacagattaa gaaagaaacc
     5101 cgtattaatg tcacggcgta tcagggcaaa gtgctgctgg ttgggcagtc accaaacgct
     5161 gaactttcgg ctcgcgccaa acagattgct atgggcgtag acggtgccaa cgaagtgtat
     5221 aacgagattc gtcagggcca gccgattggt ctgggcgaag catctaacga tacgtggatc
     5281 accaccaaag tgcgttcgca gctcttaacc agcgacctgg tgaaatcgtc caacgtgaaa
     5341 gtgaccaccg aaaacggtga agtgttcctg atggggctgg tgactgaacg tgaagcgaaa
     5401 gcggcggcag atattgccag ccgggtgagc ggcgtaaagc gggtaaccac agcgtttacg
     5461 tttattaaat agcagcccct tgtaatgcct gatgcgacgc ttgccgcgtc ttatcaggcc
     5521 tacaacgatg caaaccgtag gtcggataag gcgtttacgc cgcatccgac atgttttccc
     5581 tcaaatcaga ataacagcgc cagcccgccg acaatcacgc ctgacactgc caccatcgcc
     5641 cccgtcagcc ataaggcttt cgccgggaac gctttgcgca gcataatcag tgacggcaaa
     5701 ctcactgccg ggagcgtcat caacaatgcc agcgccggag cggttcccat acctgccagc
     5761 atcatcgttt gtacaatcgg aatttctgct gccgtgggaa tgacaaacaa gcatcctgct
     5821 accgccatcg ccaccaccca catcaggctg ttatcgacag caccatcggc atgggggaat
     5881 aaccagacgc gtgcggcacc caacaccagt actgcaagga tgtaaaccgg gatcgtactc
     5941 cagaaaagtg tccatagcgc cctgccccag cggctaaaaa aaccgctctg tgcttccggt
     6001 atgtcaattt cgaccggtgc ctgcgtttgc ggtgtttcac gcacccattt ttgcaccagc
     6061 gtcgcaatca gcaacaccat caccagcccg gccaccagac gaatcgccgc aaaaccccag
     6121 ccgaggacaa accccataaa caccagcgtc gccgggttta acaccggatt gcccatccag
     6181 aatgccagcg caccgcccat cgatacctgt tggcgacgca ttcccgccgc gaccggagcc
     6241 gcacagcagg tacacatcat gcccggcagc gaaaacagcg ttcccagcag cgtgccgcga
     6301 aagcgcgatt gtccaagcgt acgcaacaac cagtcacgcg ggatcagcac ctgaatcaac
     6361 gaaccgagga tcacccccag caccgccgct ttccataccg cgaggaaata gatcatcgcg
     6421 taatccaacg ccgcctgcca cggattagca tccgcctgcg caaggataga tttaccgata
     6481 ctgtgggttt cggcagcagt aaacgctttg ccgtagtaag gttgccattt cacataccag
     6541 agaccggcaa tgacaacgag aaagaaaagc gcgggtttcc accactgaat gggcgttgcc
     6601 gcctgagatg aagactgacc agtcatagca ttccccagga ataagttatg ataattgagc
     6661 gcgtgaatat tacgctcact atcaattctt gggaataatt attcagctct tttgcgtaat
     6721 tctgaagagc ttaaaatagt cacgccttca tgctcaggcc gcatcgcctc tgccagcatg
     6781 acccgcgcca cgtctctcgc atcaatggat ttccagttac cgggcagtaa acgaaacagc
     6841 ggcgcaaaaa gcgtttcgtt catccgctgt ttgctacgat cgcccagcaa catcgacggg
     6901 cgagcaatgg tcaatttcgg ccagttctgg gcaattaatg cttcttccat ctcccctttg
     6961 acgcggttat agaaaaacgg cgagtgggca ttggcaccca tagcactgac caccagcata
     7021 tgctgcgcgc ccagtcgccg cccggttaat gcggtatcca ctaccagcgt gtaatcggca
     7081 tgaataaacg cctctttgct ccccgcttct cgccgcgtgg tgccgagaca acaaaacaca
     7141 atgtcgatgg gttcggtgac ctgcgccaac gcgtcactca gttgcggatc atggggatta
     7201 aacaccccgg gcatatcgcc caacggacgt cgcgtcggcg cggctatggc gttaactttc
     7261 ggttcgttaa tcaacatccg cagcaggtga ccgcccacca gccccgttgc gcctgtaatc
     7321 agtacctgac tcatcttcgc tcctttacag aattgtctgc cttgcgctcc acggctcatg
     7381 caccaggctt aatagaccat gaggtaatta tccccgattg tgtgaaattc gcctcatcca
     7441 atgcaacaac gcggaggaag catgagtaag aaaattgccg ttttaatcag tgatgaattt
     7501 gaggattcag aatttacttc acccgcagac gagttccgta aagccggaca cgaagtgatt
     7561 accattgaaa aacaagcggg taaaacggtg aaaggcaaaa aaggagaagc cagcgtgacc
     7621 atcgataaat ccatcgatga agtgacgcca gcggagtttg atgccctgct gctaccgggt
     7681 ggtcattcac cggattatct gcgtggcgac aaccgttttg tcacctttac ccgtgatttt
     7741 gtgaatagcg gcaaacctgt tttcgccatc tgtcacggtc cgcagctgct gatcagcgcc
     7801 gatgtgattc gggggcgcaa actgaccgca gttaaaccga tcattattga tgttaaaaat
     7861 gcgggcgcgg aattttacga tcaggaagtc gtggttgata aagatcagct ggttaccagc
     7921 cggacaccgg acgatctgcc agcgtttaac cgcgaggcgt tacgcctgct cggtgcctga
     7981 gtcgcgtagc caaatcattt ttttaccaaa gcccagcgtg ttgtcggtga atttgatttc
     8041 atcgagccgg atttcccaca ccggggccga cagcattctg gcaaccggaa agcgacgatt
     8101 gtacgcctgg cgcgcgaggt cgctttcctc accttccagc ctgcggatct cacctttaaa
     8161 ctgcacgccg cgaattaacg ctaccgtttt cggctgaccg tttaccgttc cggcaaccgc
     8221 agcctgcggc ccactcatct gcgcgtggcg cgttttttct tccgtcaaaa tgtagaaggc
     8281 gactttctgc gcatcaaaca gataaaaagc attagcgcac cacagttccc cttcctgctg
     8341 cacacaccag gtgacaacat gttgttttgc caaccaacgg ctgatggtga tgagtgtttc
     8401 cattgctgct ctcttttata ctgtgggcct gaccttaaca tgcgacgcag acgatgacac
     8461 cctggtttct ttacttgatc cgtaccgccg acaataagct ttataccggg atcaccacgg
     8521 atgtcgaacg ccgctatcag cagcaccaaa gcggcaaagg ggcgaaagca ctgcgcggga
     8581 aaggagaact gacgctggcg ttttctgcgc cagtgggcga tcgttcgctg gcgttacggg
     8641 cggaatatcg cgttaagcaa ctgaccaaac ggcaaaaaga gcgtctggta gcggaaggcg
     8701 cagggtttgc agagctgtta agcagtctgc aaaccccgga gattaaaagc gattgaaatg
     8761 ctcgtgatac tcaaccagac cggttacgcc attcagcgcg tcatccgcta aacgatgtac
     8821 ctggaaggcg ctttcagtgc ccggccagcg gcaacgcaga tcgtgatgcg ccgccagttc
     8881 aaagccgaaa cgactgtaca gcgccggatc gcccagcgtc accactgcgg catagccgaa
     8941 ctcattaagc gaatcgagtc cttcatagac cagttggcgt gccagcccct gtccacggta
     9001 tttttcatca accgccaacg gtgccatgcc gacccattgc aggtcttcgc cctgcacatc
     9061 aaccggacta aatgccacat agccaatgac ctgaccttcg tcatctgtcg ccaccagccc
     9121 cagcgtcaga aagccatctt cacgcagatc gtgaaccagc ttcgcttccg catcactttc
     9181 gaatgagcga cgcagcaggg catcaatacc cggcgcatca atgggaattt ctactcgaat
     9241 tagcatggtt cacctaccga tgtctgtttg gtttcaggcg cggttttcat tcccgcctca
     9301 acaaaatccg ccagttgcag cagcatcatg cgtaaggctt tcggcatttg ctccagttca
     9361 atggcgtcca tcaggttttt cacatacagc cccagctccg tatcgccttc aatcaccagc
     9421 cgacgctgga agaagagcgt atccggatct tgtttacgcg ccgcaatcat cagcagatcg
     9481 ctggcgtcgg cactaaaact cacatcagct tgcgcgttct ggctgaccac cagtttgcca
     9541 ttcaccaccg aggtaaacca ttgcaggtca atatcacgca catgaatact taaccagcgg
     9601 ccttcaagaa actccagctc accatcatcc agcgcctgac ggaattgcca gcttaagacc
     9661 tgctcaagaa cctggcgttt tagcgcaaat ggcgtcagtt ttaccggtac actcaacaga
     9721 gatggcccca aatgcacaat acgggaacgc agtttatcca acacgagttt tactccctgt
     9781 ttcaacaatc atcctatttt gccatatcag aaaaataaca tagcggtata aatcaacaat
     9841 tccatatgaa attgctgcta ccaccaatac aactttaact gccttaaatc aaaaattgtc
     9901 gcagcaaggt taactaaaat cccagttcgt taacattttt gcgttttgaa agcgcaacct
     9961 tcaggaaaaa ttatggagct gctctgccct gccggaaatc tcccggcgct taaggcggcc
    10021 atcgaaaacg gcgcagatgc tgtttatatc gggctaaaag atgatactaa tgcccgtcac
    10081 ttcgccggcc ttaactttac cgagaaaaaa ttgcaggaag cggtgagttt tgtccatcaa
    10141 catcgccgca aacttcacat cgcgattaac acttttgcgc atccggacgg ttacgcccgt
    10201 tggcagcgcg ccgtggatat ggcggcgcag ctgggtgccg acgcgctgat cctcgccgac
    10261 ctcgccatgc tggagtacgc cgccgagcgt tacccgcata ttgagcgtca tgtgtcagtg
    10321 caggcttcgg cgaccaatga agaggcgatt aacttttatc atcgccattt tgatgttgcc
    10381 cgcgtggtgc tgccgcgcgt gttgtcgatt catcaggtga aacagctggc acgggtcaca
    10441 cctgtcccgc tggaagtctt tgcttttggc agcctgtgca ttatgtcgga aggtcgttgc
    10501 tatctgtcgt cgtatctgac gggtgagtcg cccaacaccg tgggcgcgtg ttctccggcc
    10561 cgtttcgtgc gctggcaaca aacgccgcag gggctggaat cccgcctgaa cgaagtgctg
    10621 atcgaccgtt atcaggatgg cgaaaacgca ggttatccga cgctgtgtaa agggcgttat
    10681 ctggtggacg gcgagcgcta tcacgcgctg gaagaaccaa ccagtctcaa taccctggaa
    10741 ctgctgccgg agttaatggc ggcgaatatt gcttcggtga aaattgaagg ccgccagcgt
    10801 agcccggcgt atgtcagcca ggtggcgaaa gtctggcgtc aggctatcga ccgttgtaag
    10861 gccgatccgc aaaacttcgt accgcaaagc gcgtggatgg agacgctcgg gtcgatgtcc
    10921 gaaggcacgc agaccaccct tggcgcgtat caccgtaaat ggcagtgaga agagcaatga
    10981 aatattcctt agggccagtg ctgtggtact ggccaaaaga ggcgctggaa gaattttatc
    11041 agcaggccgc caccagcagc gccgacgtga tttatcttgg tgaagcggta tgcagcaagc
    11101 gccgggcaac caaagttggc gactggctgg agatggcaaa atcgctcgcc ggaagcggga
    11161 agcagatagt gctctccacg ctggcgctgg tgcaggcatc ctctgaactg ggcgaactga
    11221 aacgctatgt tgagaacggt gagtttctga ttgaagccag cgatctcggc gtggtgaata
    11281 tgtgcgctga acgcaaactg ccgttcgtcg ccgggcacgc gctgaactgc tacaacgcgg
    11341 tgacgctgaa aatattgctc aaacagggca tgatgcgctg gtgtatgccg gtggagcttt
    11401 cccgcgactg gctggtgaat ctgcttaatc agtgcgatga gttggggatt cgcaaccagt
    11461 ttgaagtgga agtcctgagc tacggtcatc taccgctggc ctactccgcc cgctgcttta
    11521 ccgcgcgttc ggaagaccgc ccgaaagatg agtgtgaaac ctgctgcatt aagtatccga
    11581 acgggcgcaa cgtgctgtcg caggaaaacc aacaagtgtt tgtactcaat ggcattcaga
    11641 ccatgagcgg ctacgtttac aacctcggta acgagctgac atccatgcag ggattggttg
    11701 atgtggtccg cctgtcaccg cagggtactg acactttcgc gatgctcgac gccttccgcg
    11761 ctaatgaaaa tggcgctgcg ccactgccgt tgacggcaaa cagcgactgt aacggctact
    11821 ggcggcggct ggctgggctg gagctgcaag cctaagtaaa tatgctcgtc atacttcaag
    11881 ttgcatgtgc tgcatctgca ttcgttcacc ccagtcactt acttatgtaa gctcctgggg
    11941 attcactctc ttgtcgcctt cctgcaactc gaattattta gagcacagct cactttgtta
    12001 acaactttaa ctactcttta atgcagtatt aaagattaat cggtaacaaa gtgagctgtt
    12061 atgactgata aaaccattgc gttttcgcta ctcgatctgg cccccattcc cgaaggttct
    12121 tcagcgcgag aagcattctc ccactctctc gatctcgccc gtctggctga aaagcgcggc
    12181 tatcatcgct actggctggc agaacaccac aatatgactg gcattgccag tgctgccacg
    12241 tcggtattga ttggctatct ggcggcgaat accaccacgc tgcatctggg gtctggcggc
    12301 gtgatgttgc ctaaccactc accgttggtc attgccgaac agttcggcac gctcaataca
    12361 ctctatccgg ggcgaatcga tttggggctg ggtcgtgcgc caggtagtga ccaacggaca
    12421 atgatggcgc tgcgtcgcca tatgagcggc gatattgata atttcccccg cgatgtcgcg
    12481 gagctggtgg actggtttga cgcccgcgat cccaatccgc atgtgcgccc ggtaccaggc
    12541 tatggcgaga aaatccccgt gtggttgtta ggctccagcc tttacagcgc gcaactggcg
    12601 gcgcagcttg gtctgccgtt cgcgtttgcc tcacacttcg cgccggatat gttgttccag
    12661 gcgctgcatc tttatcgcag caacttcaaa ccgtcggcac gactggaaaa accatacgcg
    12721 atggtgtgca tcaatattat cgccgccgac agcaaccgcg acgctgaatt tctatttacc
    12781 tcaatgcagc aagcctttgt gaagctgcgc cgtggcgaaa ccggacaact gccgccgccg
    12841 attcaaaata tggatcagtt ctggtcaccg tctgagcagt atggcgtgca gcaggcgctg
    12901 agtatgtcgt tggtgggtga taaagcgaaa gtgcgtcacg gcttgcagtc gatcctgcgc
    12961 gaaaccgacg ccgatgaaat tatggttaac gggcagattt tcgaccacca ggcgcgcctg
    13021 cattcgtttg aactggcgat ggatgtgaag gaagagttgt tgggatagtg tgtcttaacg
    13081 cgggaagcct tatccgagct ggcaacgctg tcctacgtag acctgataag cgaagcgcat
    13141 caggcattgt gtaggcagca gaaatgtcgg ataaggcacc gctgattact gatacaccgg
    13201 cagcaaatta aagctcgata agatatggac cagtgcgttg ccaacgccaa acaccagaat
    13261 cagcgtaatc atcggcttgc cgccccagac acggaatttc gggctgccaa agcgtttacg
    13321 cgatgcgcgg gctaacagcg ccggaacaat tgccgcccag atggtagctg ctaaaccagc
    13381 ataaccaatg gcgtacagga atccgttcgg gaacagtaga ccacccacaa caggcggggc
    13441 aaaggtcagc aatgccgttt tcaagcgccc cagggctgag tcatcgaaac caaacagatc
    13501 tgccagatag tcaaacaaac ccagcgttac gccaaggaac gaactcgcta ccgcaaagtt
    13561 tgagaacacg accagcagaa ggtccaggct acggctgttc agcacgccgc ttaatgcctg
    13621 taccagcaca tcaatattac cgcccttctc tgcaataccg ataaactccg gacgcgggat
    13681 gttacccatc gtcgccagca accagatggt atacagcgcc agtgccatca gcgtaccgta
    13741 caccaggcat ttcacgatgg ttttcggatc tttgccgtaa tacttcatca ggctcggcac
    13801 gttaccgtga taaccaaacg atgccagaca gaacggcagg gtcatcagca aatacggcgc
    13861 ataagatgca ttgctttcgg cgacgttgaa caatgtcgca ggctgcacat gccccagtag
    13921 gctaccgaag gtgaggaaga aggtaatgac tttcgccccc agtacaatcg ccgtcatgcg
    13981 gctgacggcc ttagtactca accacaccac aaacgctacc agcagtgcaa aaccaaaacc
    14041 cgccgcccgt gccgggacgt ttagcgacat ctctgcgaag gtgtgatgca gaattgaacc
    14101 actggcagaa atataggcat aggtcaggat atagagcaca aaggcaatgg aaataccgtt
    14161 gaccacgttc cagcctttgc ccagcaaatc tttggtgatg gtgtcaaaac tcgaaccgat
    14221 tcggtaattc aggttagctt ccagaatcat caagccggaa tgcagcatac agaaccaggt
    14281 aaagatcagc gccgccattg accagaaaaa ccacgccccg gacatgacca ctggcagaga
    14341 aaacatccct gcgccaataa tggtgccgcc gataatcacc acgccgccaa gcagcgacgg
    14401 tgacgtttgg gtggtggtta gtgttgccat gagagcttct ctccagtgaa aaatagtgcg
    14461 actgcgttgt tatgcattgc actgtaccag tacacgagta caaaagacag aaaaaaagcc
    14521 ccgatggtaa aaatcggggc tgtatatatt attttacaga ttgtgttcgc tgttcagcga
    14581 tgattacgca tcaccaccga aacgacgacg accggtagag tcatcacgac gcggagcgcg
    14641 gccttcacga cgttcgccgc taaaacgacg accatcacca cggccacctt cacggcgttc
    14701 accgctgaag ttacgaccgc cttcacgacg ttcgccaccg aaaccacgac caccgccacg
    14761 acgctcaccg ccagtatgcg gctgtgcatc gcccagtaac tgcatgttca tcggcttgtt
    14821 gagaatgcga gtgcgcgtaa agtgttgcag cacttcaccc ggcatacctt tcggcagttc
    14881 gatggtggag tgagaagcaa acagcttgat gttaccaatg taacggctac tgatgtcgcc
    14941 ttcgttagca atcgcaccaa cgatatgacg aacttcaaca ccatcatcgc ggcccacttc
    15001 aatgcggtac agctgcatat cgccaacatc acgacgttca cgacgcggac gatcttcacg
    15061 gtcaccacgc gggccacggt cgttacgatc gcgcggacca cggtcatcac ggtcacggaa
    15121 ttcacgtttc ggacgcatcg gcgcatctgg cggtacgatc agagtacgtt caccctgtgc
    15181 cattttcagc agtgccgcag ccagagtttc gagatccagc tcttcacctt ctgcagtcgg
    15241 ctgaattttg ctcagcagtg cgcggtattg atccagatcg ctgctttcca gctgctgctg
    15301 tactttagcg gcgaattttt ccagacggcg tttgcccagc agttctgcgt tcggcagttc
    15361 tacttccgga atagtcagct tcatagtacg ttcaatgttg cgcagcagac gacgctcgcg
    15421 gttctcaacg aacagcagcg cgcggccagc acgacccgca cgaccggtac gaccgatacg
    15481 gtgaacgtaa gactcagaat ccatcgggat atcgtagtta actaccaggc tgatacgctc
    15541 aacgtccagg ccacgggctg caacgtcggt cgcaatcagg atgtccagac gaccatcttt
    15601 caggcgttcc agtgtctgtt cacgcagcgc ctggttcatg tcaccgttca gcgcggcgct
    15661 gttgtagccg ttacgctcaa gagcttcagc cacttccaga gtcgcgtttt tggtacgaac
    15721 gaagataatc gccgcatcaa aatcttccgc ttccaggaaa cgtaccagtg cttcgttttt
    15781 gcgcataccc cagacagtcc agtagctctg gctgatgtca ggacgggtag tcacgctgga
    15841 ctgaatgcgc acttcctgcg gctctttcat aaagcggcgg gtaatgcgac gaatcgcttc
    15901 cggcatggtt gcagagaaca gagcggtctg atgaccttcc gggatctgcg ccataatggt
    15961 ttcaacgtct tcgatgaagc ccatgcgcag catttcgtca gcttcatcca gaaccagacc
    16021 gctcagttta gagaggtcca gagtgccacg tttcaggtgg tccagcagac ggcccggagt
    16081 accgacaacg atctgcggcc cctgacgcag ggcgcgtaat tgcacgtcat aacgctggcc
    16141 gccgtacaga gcaaccacat ttacgccgcg catgtgttta gagaaatccg tcattgcttc
    16201 agcaacctgt accgccagtt cgcgggtcgg tgccagcacc agaatctgtg gtgctttcag
    16261 ctcaggatca agattctgca acagaggtaa agagaatgct gcagtttttc cgctccccgt
    16321 ctgggccata cccagaacgt cgcggccatt cagcagatgt ggaatacact ctgcctgaat
    16381 tggagatggt ttttcgtaac ccagatcgtt aagggcttca aggataggag ccttcaggcc
    16441 cagatctgca aaagtggttt cgaattcagc catgtagtac gtgtgcctca aaattaatgg
    16501 cggccagtct acataactca tcatgaaatt gatcagcaat tttcattgaa aagtgtgaac
    16561 cggctcaaag taggtgtatt aacgaacaac aacgccctca cccgttaagg tgatggcaat
    16621 caaaaaagat tacgggctga tgtgtacgtc agctattgct ggtccgattc tgccaggtca
    16681 tcttggtcct ggcccaggag cgataattcc aacaatgcgt atcggtgctc aacaaagtta
    16741 tgaacgttgt tggcaaccgc cagtttgaac agtgccgtgg cgctgtccaa atcccccaga
    16801 cttaggtagt acttacctaa atagaagttg gtttcactga gatgctcagc gagcgaggtg
    16861 ttatccgttg cgtccgcctt gagcctttcc attaacgttt gttcgctaat gttgcccagg
    16921 tagaactcga caatgttcca tccccactgt tccttatccg atttttcgaa gtgctgtttc
    16981 aacacttctt tagcctgctt ctcatcgagc ttctgctcgg cgagataaag ccacagacta
    17041 cggaaaggat cattgggatc gtcttgataa aacgccagca gatcatcttg cgctaacttg
    17101 tcacgaccgc cgtaatataa tgcgatcccg cgattcaagt gcgcgtagtt gtaagttgga
    17161 tcaagctcaa gtacagaatc aaacgcttca taggcagcat caaaattgcc tgcctgcgtt
    17221 aaatatatgc ctaagtaatt gaatacttca ggcatatccg gtcggattgc cagcgcttgc
    17281 gaaaaatcgt tacgcgctaa tgccctcaga ccgagactat catacaacac tccgcgctca
    17341 tataaaagct gtgcgcgttc gtcatcggtt aaagcccgac tggcaaggat ttgttccata
    17401 cgtgccagaa tcacttcctg ctgtaaagtc ggttgcaatg gtaccgcgag gacttcactt
    17461 ttacgccagg aagtattact gcatcctgca agcgtaagtg ctgtcgcaac gaaacaccag
    17521 cgcaaaaaag gcttcatttc ccactcccga agaccacggt tgaatgaacg tcctgttccc
    17581 ggttgctaac aaggcgtcct gcccggttaa aagccccccg ccgcagcgga gggcaaatgg
    17641 caaccttact cgccctgttc agcagccgga gcttccggtg ctgcagcagg ttgagactgc
    17701 tcagtcgctt ctttaatgct cagacggata cggccctggc gatcaacttc tagaactttc
    17761 accggtactt cctgacccat ctgcaggtaa tcggtcactt tctcaacgcg tttgtcagcg
    17821 atttgagaga tgtggaccag accttcttta ccgccgccga tggcaacaaa tgcgccaaag
    17881 tcaacgatac gggtcacttt accagtgtag acgcggccca cttcgatttc tgcagtgatc
    17941 tcttcgatac gacgaatagc atgtttcgct ttctcgccgt cggtcgctgc gatcttcaca
    18001 gtaccgtcat cttcgatttc gatggtagtg ccagtttctt cggtcagggc acggataaca
    18061 gagccgcctt taccgataac gtctttgatc ttatccgggt tgatcttgat ggtatggata
    18121 cgcggtgcga actcagagat atcgccacgc ggcgcgttga tcgcctgttc cattacgccc
    18181 aggatatgca gacgcgcacc tttagcctgg ttcagcgcaa cctgcatgat ctctttggtg
    18241 ataccttcaa ttttgatatc catctgcagt gcagagatac cgtcgcggga acctgcaact
    18301 ttgaagtcca tatcgcccag gtgatcttcg tcgcccaaaa tgtcagacag tacaacgtag
    18361 ttgtcgcctt ctttcaccag acccattgcg atacccgcaa cggcagcttt gattggcaca
    18421 cctgcgtcca tcagcgccag agacgcgccg cacacggaag ccatagaaga agaaccgttg
    18481 gattcggtga tttcagacac tacacgtacg gtgtacggga atttgtccat atccggcatg
    18541 actgccagca cgccgcgctt cgccagacga ccgtgaccaa tttcacgacg cttcggagaa
    18601 ccgaccatgc cggtttcgcc tacggagtac ggagggaagt tgtagtggaa caggaaggta
    18661 tcggtacgtt cgcccatcag ttcatcaaga acctgcgcgt cacgagcggt acccagcgtt
    18721 gcagtaacca gtgcctgcgt ttcaccgcgg gtgaacagcg cagaaccgtg agtacgcggc
    18781 agcacgccag tacgcacatc cagaccacgg atcatatctt tttcacgacc gtcgatacgc
    18841 ggttcgcctg ccagtacgcg gctacgaaca acgtttttct cgatagcgtg cagaatttca
    18901 cccagttcgt tttcgtccag ggtttcgtct tcagcaagca gcgtcgcgat ggtttcagat
    18961 ttgatgacat caacctgcgc ataacgctct tgtttgtcgg tgatgcggta agcatcactc
    19021 agacgagctt cagccagtgc agcaacgcgc gcgttcagcg cttcgtttac cggctccggc
    19081 tgccagtccc aacgcggttt accggcttct ttcactagtt cattgatgtt ctgaataaca
    19141 acctgctgtt gttcatgacc gaacactact gcgcccagca tctgatcttc gctcagcagt
    19201 tcagcttcag attcaaccat cagtacagcg gcttcagtac cggcaacaac cagatccagt
    19261 ttgctctctt tcagctcgtc ctgagtcggg ttcagtacgt actggtcatt gatgtaacct
    19321 acgcgggcag caccaatcgg gccattgaac ggaataccag acagagacag tgctgcggaa
    19381 gcaccaatca tcgcgacgat atccgggtta acctgcgggt taacagaaac cacggtggcg
    19441 ataacctgaa cttcgttgac gaagccttcc gggaacagcg ggcgaatcgg gcggtcaatc
    19501 agacgcgcga tcagggtttc gccttcgctt ggacgacctt cacgacggaa gaagctaccc
    19561 gggatacgac cagcagcgta ggtacgctcc tgatagttaa cggtcagtgg gaagaagtcc
    19621 tgacctggtt tggctttttt ctgtccaaca acggtaacga ataccgcggt gtcatccatg
    19681 ctaaccataa cagcggcagt agcctgacga gccatcatgc cggtttccag agtcacggtg
    19741 tgttggccgt actggaattt acgaacgatc ggattaagca atgtaatatc ctttctcttt
    19801 cttagacagt accttacggc actggtgtta atacccgatc ttctgcgcat cctcgcgact
    19861 aatgacaacc ctaacccagc tctatgtggg taaagcctct cattagccgc gcgaacctct
    19921 gcaacggaag atcattcata gcaacaatac attagtttcc agtgaattgc tgccgtcagc
    19981 ttgaaaaaag gggccactca ggcccccttt tctgaaactc gcaagaatta gcgacgcaga
    20041 cccaggcgct cgatgagctg ggtgtaacgt gctacgtctt tacgtttcag gtagtcgagc
    20101 agtttacgac gctgagaaac catgcgcagc agaccacgac ggctgtggtg atcttttttg
    20161 tgctctgcaa agtggccctg caggtggttg atctgtgcag tcagcagtgc tacctgaact
    20221 tcggtagaac cggtgtcgtt tgcgtcacga ccaaactcag aaacgatttt agctgttgct
    20281 tcagtactta gagacatttt aaaactccaa agtatataga atgaaaggac gccgatctct
    20341 aattcagcga tcccagtgta cgttacgcaa agtgttaaac aatttacgcg acgttaagcg
    20401 gcagtattct actcgtagcg acctgttatc gcaagacggt taacattacg ccgggtattc
    20461 aaccaccagg cgacgaggcg cgacgcggcc ttcatcgtca atttcgccca taccgataaa
    20521 cttgccgttc tcaccttctg taacgcgaac cagtccttcc agcggcgcac cggatgtacg
    20581 aactgggtta ccatttttga agtaaacaga agacgttaac ggaagattca ccaccgggta
    20641 gtccgaagct ggactgtcca ttggcatcag taatggatca agtaactccg cagccggaat
    20701 atcctgctgt tcggcttgtt cgaccagttc acgcagatgt tccagggtca ccatccgttc
    20761 aaccggatat ttacttaccg ccagacgacg caggtaaata acatgcgcgc cacaaccgag
    20821 tttttcaccc aggtcatcaa tgatggtgcg gatataagtg ccttttgagc agtgaatttc
    20881 cagctccagc tcattgcctt cgtggcgaat aaacagcaat tcataaacgg taatcgggcg
    20941 cgcttcacgc ggtacttcaa tgccctgacg cgcatattcg tacagttttt taccctgata
    21001 tttcagtgct gaatacatcg aagggatctg ttcgatatcg ccacggaaag tatccagcgc
    21061 cgccgccagt tgctctgcgc taaaggttac cggacgttct tcaacgattt gtccgtcggc
    21121 atcagaagta tcggtacgct gtccaagacg cgcaatgacc cgatagcgtt tgtcggagtc
    21181 cagcagatac tgggaaaact tcgtcgcttc cccgaggcaa attggcaaca tgcctgttgc
    21241 cagcgggtcc agcgcaccgg tatgcccggc acggttggcg ttataaatcc gtttcacttt
    21301 ttgcagcgca tcattgctgg acataccctg cggtttatcc agcaacaaaa cgccgttaat
    21361 gtcgcgaccg cgacgacgag gacgactcat tagtcctcct tgctgtcgtc cgggttaaca
    21421 cgacgttctt cgtcatgttt gaccacgctg gtcaccaggt ttgacatgcg catcccttca
    21481 accagagagt tgtcgtagaa gaaggtcagt tccggcacga tacgcaggcg catcgctttc
    21541 cccagcaggc tgcggatgaa accagaagct tcttgcaacg ctttgatgcc cgctttaacc
    21601 gcgtcttcat ctttgtcgtt gaggaacgtc acatatactt tggcatacgc caggtcgcga
    21661 gacatttcga caccggaaac ggtggtcatc atgcccaggc gaggatcttt aatttcacgc
    21721 tgcaggatga gagcgatctc tttttgcatt tcctgcgcta cgcgctgcgg gcgaccaaat
    21781 tctttcgcca taataaattc tcctgacaaa aaaggggctg ttagcccctt tttaaaatta
    21841 atttcaggtg gaagggctgt tcacgttggc ctgataagac gcgccagcgt cacatcaggc
    21901 aatccatgcc ggatgcggcg taaacgcctt atcccgcatg gaaccctaaa aaccttaagc
    21961 gatggtacgt tggatctcga tgatttcgaa tacttcgatc acatcgccag tgcggacgtc
    22021 gttgtagttc ttaacgccga taccacattc cataccgtta cggacttcgt taacgtcatc
    22081 tttgaagcgg cgcagggact ccagctcgcc ttcgtagata accacgttgt cacgcagaac
    22141 gcggatcggg ttgtgacgtt taaccacacc ttcggtaacc atacagcctg cgatagcacc
    22201 aaatttcggc gatttgaaca cgtcacgaac ttccgccaga ccgataatct gctgtttcag
    22261 ttccggagac agcataccgc tcatcgccgc tttcacttcg tcaatcaggt tatagatgac
    22321 ggagtagtaa cgcagatcca ggctttccgc ttcaatcact ttacgtgcag aggcatcagc
    22381 acgtacgttg aagccaacca ggatggcgtt ggacgccgca gccagggtgg cgtcggtttc
    22441 ggtgatacca cctacgccag aaccgatgat cttcacttta acttcgtcag tagacagttt
    22501 cagcaaggag tcggagatcg cttccacaga accctgtacg tctgccttca ggacgatgtt
    22561 cacttcgtga acttcgcctt cggtcatgtt ggcgaacatg ttctcgagtt tagatttctg
    22621 ctgacgcgcc agtttaactt cgcggaattt accctgacga tacagtgcaa cttcacgcgc
    22681 tttcttctcg tcacgtacaa cggtaacttc atcacccgca gccggtacgc cggacaggcc
    22741 gaggatttcc accggaatgg acggacccgc ttccagcact tcctgaccca gttcgttacg
    22801 catcgcacga acacgaccgt attcgaagcc acacagaacg atatcgccct tgtgcagagt
    22861 accttcacgt accagaacgg tagcaaccgg accacgacct ttatcgagga aggattcgat
    22921 aaccgcaccg ctcgccatac ctttacgtac cgctttcagc tccagaactt ccgcctgcag
    22981 caggatagcg tccagcagtt catcgatgcc ggtacccgct ttcgcagata cgtgtacgaa
    23041 ctggctttca ccgccccact cttccggcag gatgccatac tgggagagtt cgttcttaac
    23101 acgatccgga tcggcttctg gtttatcaat cttgttcact gcaaccacca ccggtacctg
    23161 cgccgctttc gcgtgctgga ttgcttcgat ggtctgcggc atcacgccgt cgtcggcagc
    23221 aacaaccagg actacgatgt ccgttgcctg cgcaccacgt gcacgcattg aagtaaacgc
    23281 ggcgtgcccc ggggtgtcca ggaaggtgat catgccgttt tcagtttcaa cgtggtatgc
    23341 accaatgtgc tgggtaatgc cgcccgcttc gccagaggca actttcgttg aacgaatgta
    23401 gtccagcaga gaggttttac cgtggtcaac gtgacccatg atggtcacaa ccggcgcgcg
    23461 cggttcagcc gcagcacccg tgtcacggtc gctcattacc gcctcttcca gctcgttttc
    23521 acgacgcagg ataactttgt ggcccatctc ttcagcaacc agctgtgcgg tttcctgatc
    23581 gataacctgg ttgatggttg ccattgcgcc cagtttcatc atcgctttga tgacctgaga
    23641 gcctttaacc gccatcttgt tcgccagttc gccaacggtg atggtttcgc cgatcacaac
    23701 gtcacggtta acggcctgag ctggcttctg gaagccttgc tgcagggaag aacctttacg
    23761 ttttccgcct ttaccgccac gtactgctgc gcgtgcttct tcacgatcag cttttgattc
    23821 agcgtgtttg ttgcctttct tcggacgcgc tgctttcgcg ttacgaccac ggccacgacc
    23881 gccttcgact tcacgatcgc tttcgtcttc tgcctggcga gcatgttgag aagtagtgac
    23941 gtgataatcg ctggaatctt cagtcggttc cgcgttatca gtccatttgt tttcttccgc
    24001 catacgacgt gcttcttcag caacgcgacg tgcttcttct tcgagtttac gatgcgcttc
    24061 ttcttcggct ttacgcttga gctctgcagc ttcctgctca cggcgggctt tttcagcctg
    24121 ggcgttttta gtcatatcgt cttgttgatt gctcactttg tctttttccg cagcttcacg
    24181 tttcgcttgt tcagcagctt cacgcttagc ttgttctgca gcctcacgtt cagctttttg
    24241 ttgcgcctcg cgtttagccg attcttctgc ctcacgacgg gcttgctctt ccgcttcacg
    24301 ctgcgcttgc tcttccgctg caaggcgttc agcctcttgc ggatcgcgtt tcacaaaggt
    24361 gcgtttcttg cggacttcga tttgtaccga tttgcttttt ccaccggtac caggaatgtt
    24421 aagggtgctg cgtgttttac gttgcagcgt caatttgtcc gggcctgaat ttttctgatt
    24481 caggtggtca atcaaagtct gtttctcttg tgcagacaca gagtcgtcag cagacttccg
    24541 gatacctgca tcagcaaatt gctgtaccag gcgttccacg gaggtctgtc gctctgcggc
    24601 cagcgtttta atcgttacat ctgtcatgct gttccttcct gctacagttt attacgcttc
    24661 gtcaccgaac cagcaaatat tacgggcagc cataatcagt gctccggcct tttcgtcggt
    24721 caacccttcg atatcagcca gatcatcaat gccctgttcg gcgagatctt ccagcgtaca
    24781 aacgccacgg gcggccagtt tgaatgccaa atcacgatct accccttcaa ggttcagcag
    24841 atcgtcagcc ggtttgttat caccgaggct ttcttcctgg gcctgtgcaa tggtggccag
    24901 tgcattttta gcacgctcgc gcagtgcttc aacggtcggc tcatcaaggc cttcgatttc
    24961 caacagctct ttcatcggca cataggccag ttcttccagc gtcgagaagc cttcttctac
    25021 cagaacagtc gcgaagtctt cgtcgatgtc gagatatttg gtgaaggtgt cgatcgctgc
    25081 gtgcgcttcc gcctgatgct tagcctgcag gtcgtcaacg gtcatcacgt tgagttccca
    25141 accgctcagc tgcgaagcca gacgcacgtt ctgaccgtta cggccaatcg cctgcgccag
    25201 gttaccggct tcaacggcga tatccatggt gtgtttatct tcatccacca cgatagaagc
    25261 aacgtctgcc ggtgccattg cgttaatcac gaactgcgcc gggttatcat cccacaggac
    25321 gatatcgata cgctcgccac ccagttcagt agacaccgcc tgaacacgcg cgccacgcat
    25381 acctacgcaa gcacctaccg gatcgatacg tttatcgttg gttttcaccg cgattttcgc
    25441 acgagaaccc ggatcgcgag ccgctgcttt aatttcaatc acttcttcgc cgatttctgg
    25501 cacttcaata cggaacagtt cgatcagcat ttccggcttg gaacgagtga cgaacagttg
    25561 cgcgccacgc gcttccgggc gaacggaata aagcacgcca cgaacgcggt cgccagggcg
    25621 gaagttttca cgcggcagca tatcttcacg caggatcacg gcttcagcgt tgttgcccag
    25681 atccagagag atgttgtcgc ggtttacttt tttcaccacg ccggtgatga tttcaccttc
    25741 gtgttcacgg aactgatcaa ccaccatcgc acgttcggct tcacgcactt tctgcacgat
    25801 aacctgtttt gccgtctggg tagtgatacg gtcaaaggta acagactcaa tctgatcttc
    25861 aacgtaatcg cccaggttca ggctttcatc ttcataacgt gcggcttcaa gggtgatttc
    25921 cttggtcggc tgggtgactt catcaacaac taaccagcga cggaaggtgt caaaatcacc
    25981 gcttttgcga tcgatctgta cgcggacgtc gatctcttgt tcatattttt tctttgttgc
    26041 tgtcgccagc gcgctttcca atgcttcgaa aatcttctcg cgaggtagcg ccttttcatt
    26101 ggatacggct tcaactacag ccaaaatttc tttgttcatc gcgggctttt cacctcatcc
    26161 agactattaa aagtggggaa ccaggttcgc cttctggata ttactcagcg cgaacacttc
    26221 atctttacct tcgacggtaa ctgtgatcat ttcaccgtct accgctttga taacgccctg
    26281 ccatttacga cggttttgta ccgccatacg gagaaccaga gtcacctctt ctccgacaaa
    26341 acgggcgtag tgttcagccg tgaacagtgg gcgatcgaga cccggtgagg agacttccag
    26401 gttataagca acggtgatgg gatcttcaac atccagcaca gcacttacct ggtggctcac
    26461 atcagcacaa tcatcaacat tgatgccatc ttcactatca atatagatgc gcagtgtgga
    26521 tgtgcgaccg cgaataaatt cgatgccaac cagttcaaaa cccagggcct caactggcgc
    26581 agtaatcatc tctgttaatt tttgctctaa tgtggacaag cccaccccca agacataaaa
    26641 aaagggccta aagcccagtt attctgtagt cagataacaa aaaaccccga taaatcgggg
    26701 ctttatataa ctgaacccta taaccgcaac tgcggtctgg agcactttcc agaaggattt
    26761 tttcaaatcc cactacgaag gccgaagtct tcacagtata tttgaaaaag gactctaagg
    26821 gaaagtggtt gcgggggccg gatttgaacc gacgaccttc gggttatgag cccgacgagc
    26881 taccaggctg ctccaccccg cgcctgaaac gtggcaaatt ctactcgttt tgggtaaaaa
    26941 atgcaaatac tgctgggatt tggtgtaccg agacgggacg taaaatctgc aggcattata
    27001 gtgatccacg ccacattttg tcaacgttta ttgctaatca tgtgaatgaa tatccagttc
    27061 actttcattt gttgaatact tttgccttct cctgctcccc ctta
//
LOCUS       DAEEKQ010000042        26772 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.042, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000042 DAEEKQ010000000
VERSION     DAEEKQ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26772
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.042"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            110..382
                     /locus_tag="KLK57_001692"
     CDS             110..382
                     /locus_tag="KLK57_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HBE0892077.1"
                     /translation="MGLSSAAFAAETATTPAPTATTTKAAPAKTTHHKKQHKAAPAQK
                     AQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQPAKPAAQPAA"
     gene            475..558
                     /locus_tag="KLK57_001693"
     CDS             475..558
                     /locus_tag="KLK57_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892078.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            658..1479
                     /locus_tag="KLK57_001694"
     CDS             658..1479
                     /locus_tag="KLK57_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HBE0892079.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTNDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(1518..1847)
                     /gene="mdtI"
                     /locus_tag="KLK57_001695"
     CDS             complement(1518..1847)
                     /gene="mdtI"
                     /locus_tag="KLK57_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HBE0892080.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(1834..2199)
                     /gene="mdtJ"
                     /locus_tag="KLK57_001696"
     CDS             complement(1834..2199)
                     /gene="mdtJ"
                     /locus_tag="KLK57_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HBE0892081.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            2611..3645
                     /gene="tqsA"
                     /locus_tag="KLK57_001697"
     CDS             2611..3645
                     /gene="tqsA"
                     /locus_tag="KLK57_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HBE0892082.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLISVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            complement(3670..5058)
                     /gene="pntB"
                     /locus_tag="KLK57_001698"
     CDS             complement(3670..5058)
                     /gene="pntB"
                     /locus_tag="KLK57_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HBE0892083.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(5069..6601)
                     /gene="pntA"
                     /locus_tag="KLK57_001699"
     CDS             complement(5069..6601)
                     /gene="pntA"
                     /locus_tag="KLK57_001699"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HBE0892084.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            7125..8069
                     /locus_tag="KLK57_001700"
     CDS             7125..8069
                     /locus_tag="KLK57_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0892085.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEILGVATTA
                     SPSSEVGRFFETQSSKGGRYTVMLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            8255..9637
                     /locus_tag="KLK57_001701"
     CDS             8255..9637
                     /locus_tag="KLK57_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0892086.1"
                     /translation="MKKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            9674..10396
                     /gene="folM"
                     /locus_tag="KLK57_001702"
     CDS             9674..10396
                     /gene="folM"
                     /locus_tag="KLK57_001702"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HBE0892087.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSPHGLRAILHNASAWMAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHIAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(10393..10728)
                     /locus_tag="KLK57_001703"
     CDS             complement(10393..10728)
                     /locus_tag="KLK57_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HBE0892088.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVSCWGISAWV
                     LIICWIKLH"
     gene            10857..11576
                     /gene="rstA"
                     /locus_tag="KLK57_001704"
     CDS             10857..11576
                     /gene="rstA"
                     /locus_tag="KLK57_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HBE0892089.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            11580..12881
                     /gene="rstB"
                     /locus_tag="KLK57_001705"
     CDS             11580..12881
                     /gene="rstB"
                     /locus_tag="KLK57_001705"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HBE0892090.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALITFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            12957..13886
                     /gene="tus"
                     /locus_tag="KLK57_001706"
     CDS             12957..13886
                     /gene="tus"
                     /locus_tag="KLK57_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HBE0892091.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(13883..15286)
                     /gene="fumC"
                     /locus_tag="KLK57_001707"
     CDS             complement(13883..15286)
                     /gene="fumC"
                     /locus_tag="KLK57_001707"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HBE0892092.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQVKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(15429..17075)
                     /gene="fumB"
                     /locus_tag="KLK57_001708"
     CDS             complement(15429..17075)
                     /gene="fumB"
                     /locus_tag="KLK57_001708"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBE0892093.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            17274..18449
                     /gene="manA"
                     /locus_tag="KLK57_001709"
     CDS             17274..18449
                     /gene="manA"
                     /locus_tag="KLK57_001709"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HBE0892094.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            18550..20058
                     /locus_tag="KLK57_001710"
     CDS             18550..20058
                     /locus_tag="KLK57_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HBE0892095.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(20239..21549)
                     /gene="uidC"
                     /locus_tag="KLK57_001711"
     CDS             complement(20239..21549)
                     /gene="uidC"
                     /locus_tag="KLK57_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HBE0892096.1"
                     /translation="MRKIVAMAVICLTAASGLISAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKSGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAQIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLKSEITPERKTSGVSDC"
     gene            complement(21588..22961)
                     /gene="uidB"
                     /locus_tag="KLK57_001712"
     CDS             complement(21588..22961)
                     /gene="uidB"
                     /locus_tag="KLK57_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HBE0892097.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGVLFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSRKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            complement(22958..24769)
                     /gene="uidA"
                     /locus_tag="KLK57_001713"
     CDS             complement(22958..24769)
                     /gene="uidA"
                     /locus_tag="KLK57_001713"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HBE0892098.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYAGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            complement(25158..25748)
                     /gene="uidR"
                     /locus_tag="KLK57_001714"
     CDS             complement(25158..25748)
                     /gene="uidR"
                     /locus_tag="KLK57_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HBE0892099.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIKGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            complement(25969..26736)
                     /gene="hdhA"
                     /locus_tag="KLK57_001715"
     CDS             complement(25969..26736)
                     /gene="hdhA"
                     /locus_tag="KLK57_001715"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005153726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HBE0892100.1"
                     /translation="MRHAPCAMRHAPCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAISKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
BASE COUNT         6716 a         6939 c         6435 g         6682 t
ORIGIN      
        1 ccattcccag ggatatagtt atttcaacgg ccccgcggtg gggttaaatg aaaaaacaaa
       61 ttgagggtat gacaatgaaa aagtattagc tctggttgtt gccgctgcta tgggtctgtc
      121 ttctgccgcc tttgctgcag agactgcgac cacacctgct ccgactgcga cgaccaccaa
      181 agcagcgccg gcgaaaacga cacatcataa aaaacagcat aaagcagcac ctgcccagaa
      241 agcgcaggcg gctaaaaagc atcataaaaa tacgaaagct gaacagaaag cccctgaaca
      301 aaaagcgcag gcagcgaaga aacacgccgg gaaacacagc catcagcaac cggcaaaacc
      361 tgctgcacaa cccgcagcgt aagttttcaa cagtaatgct ggcgcgcccc ctcgcgcctg
      421 aaaattacgg tgctaagcgg gtaacgttta gcaccgcctt tagcggaggg tagtatggtg
      481 ggccgttatc gctttgagtt cattcttatc atccttattt tatgcgcact gattgccgcc
      541 cgtttttatc tttcctgact gtagttatct gattttactc ccactttcat cccgtcccgt
      601 ctatagtatt tatgagggtt tgcttttaat aatcataatt acccaccaga gtgtgatatg
      661 cgtacaacca ttgctgtagt gttgggtgca attagtttga cgtctgcttt tgtgtttgca
      721 gataaaccag acgttgccaa atcggcaaac gatgaggtca gcaccctgtt ttttggtcat
      781 gatgatcgtg tgccagtgaa tgacacgacc caatcaccgt gggatgcggt tgggcaactg
      841 gaaacggcca gcggcaattt atgtacggcg acgctgattg cacccaatct ggcattaacg
      901 gcaggacact gtttattgac acctccaaag ggtaaagccg ataaagcagt ggcgctgcgt
      961 tttgtgtcaa ataaaggtct ttggcgctat gagatccacg acatagaagg ccgcgttgat
     1021 ccgacactgg gaaagcggtt aaaagcagat ggggatggtt ggattgtacc tcccgcagcc
     1081 gcgccgtggg acttcggatt gattgtgcta cgtaatcccc cttctggcat tacaccgttg
     1141 ccgttatttg agggagataa agccgcgctt actgccgcat taaaatcggc tggccgtaaa
     1201 gtaactcagg cgggataccc tgaagatcat ctcgatacgt tgtacagtca tcaaaactgt
     1261 gaagtgactg gctgggcgca aacgtcggtg atgtcacatc agtgcgatac cttgccgggt
     1321 gacagcggtt cgcctctgat gttgcatacc aatgacggct ggcaattaat tggggtgcaa
     1381 agttcggctc ctgccgcgaa agatcgctgg cgcgccgata accgggccat ttctgttacc
     1441 ggttttcgcg acaagctgga tcaactgtcg caaaaataat gttcaaacgc tgcccgactg
     1501 tgcgggcagc gtgttcatca ggcaagtttc accatgatca ttccagccaa cagcaagacc
     1561 aggccaatcc agcctttacg atttaaccgt tgaccaaaca agatccaacc tgcggctaac
     1621 gtggcggcaa taccaaaccc gccccacaat gcataagcga cagacaagtc gatcccttta
     1681 acggcctgag aaagcgcact aaaggcagcc agcaccgccg ccagggagag caagccaaat
     1741 attttgcgac gaaagccgtc agaaaatttc aaaaagacgt tagcaacgat ttccagcacg
     1801 attgccaatg ccagccaggc ggcgtgaacc cattcaaact gcgccatggt tcacctccag
     1861 ttcaggttta cgcgctttac gggtacctga ttttatcaac acaatcccgg cgaccagggt
     1921 ggttaacccg gcaattttca tcagcgataa actttcgtcg aataacaaaa cgctaaacaa
     1981 ggtaataaat aaaataccga taccttccca cagcgcataa gctacgccta aggcgatctt
     2041 tttaacggcg aaagagagaa atatatacga cagagaaatc atcaccagca ttaaaataaa
     2101 gccgccattt ccctcactga cgctcgccca tttcattgac agcgtaccgg taatttctgt
     2161 agcaatagcc aggcctaata aaatccaata aatatacatt gtccttctcc tgcaagagaa
     2221 ttattttaat tttcgcttaa ttcagcgaaa ccaaagtaaa gtggtcaagc cccgcgcata
     2281 gggcaagctt aggcagaaga aaggactaaa gcgcgttgcg ccagtgctgc tcacctacga
     2341 gcaagatagt ggatgaggta cgaagatggg taaatgttga aaataacgtc ctgaacaaat
     2401 tgtccataat attacaatta tccgcagtgt tgcttctcgt catcgcggat gataattgtc
     2461 ctcggtagtt gaacacgcct gatttgtatc atagcttaag aattaactca aaatattttc
     2521 acttctttac ctgagcggtt tgattttcgt tatgatgacg gagcgaaaaa gacattatta
     2581 ttagcaaagg aagaaaaaac ggggacaagc atggcaaagc cgatcatcac gctcaatggc
     2641 ctaaaaatcg tcattatgtt gggaatgctg gtcattattc tctgcggtat ccgttttgcc
     2701 gccgagatca tcgtgccgtt tattctcgca ttatttattg ctgttattct taacccgctg
     2761 gtgcaacaca tggtccgctg gcgagtgccg cgtgtactgg cggtgtcgat tttgatgacc
     2821 atcatcgtga tggcgatggt gttgctatta gcttatctgg gttccgcgct caacgagttg
     2881 acgcggacgt taccgcaata tcgcaactct attatgacgc cgctgcaagc tcttgagccg
     2941 ttgttgcaac gcgtagggat tgacgtctca gttgaccagc tggcgcatta cattgatccg
     3001 aacgcggcga tgacgttgct caccaactta ttgacgcagt tatctaatgc catgtcatca
     3061 atatttttat tgctgctgac ggtgctgttt atgctgctcg aagtgccaca attgcccgga
     3121 aaatttcagc aaatgatggc gcgtccggtt gaagggatgg cggcgattca acgtgcaatt
     3181 gacagtgttt ctcattatct ggtgctgaaa acagccatca gcatcatcac cggcctggtc
     3241 gcctgggcga tgctcgccgc actcgatgtt cgcttcgctt ttgtctgggg attgctggcc
     3301 tttgcgctta attacatccc taatattggt tcagttctcg cggcaatccc ccctatcgct
     3361 caggtactgg tgtttaatgg cttctacgaa gcgttgctgg tgctggcggg atatctgctg
     3421 attaatctgg tcttcggcaa tattctggag ccgcgcatca tgggacgtgg gctggggctt
     3481 tccacattgg tggtattttt gtcgttgatt ttttggggat ggttgttagg accggtgggt
     3541 atgctgattt ccgtgccgtt aacaattatt gtcaaaattg cgcttgaaca aacagcggga
     3601 ggtcaaagca tcgccgttct gttaagcgat ctcaataaag agtgacggcc tccgcagagg
     3661 ccgtcagggt tacagagctt tcaggattgc atccacgcta gctttggcgt caccaaacag
     3721 catgtgggtg ttttccttga agaacagcgg gttttgcaca ccagcatagc cagtgttcat
     3781 cgaacgttta aagacaatca cgttctgcgc tttccacact tccagcacag gcataccagc
     3841 aataggactc ttcggatcat cctgcgccgc cgggttaacc gtatcgttag caccaatcac
     3901 cagtacggta tcggtatcag caaagtcatc gttgatctcg tccatttcca gcacgatgtc
     3961 atacggtact tttgcttcag ccagcaatac gttcatatgt ccaggcaaac gccccgcaac
     4021 cgggtggata ccgaaacgca cgttgatacc acgagcgcgc agtttctcgg taatttcagc
     4081 gacaggatat tgcgcctgcg cgactgccat gccgtacccc ggagtaatga tcactgaatg
     4141 ggagtttttc agcagttccg ctgtctcttc tgcggtgatt tcgcggtgct cgcccacttc
     4201 ctgatcatcg ccagtagaag agccgtcggt gccgaaacca cccgcaataa cgctgataaa
     4261 ggaacggttc atcgccttac acataatgta agaaaggata gcccccgaag aaccgaccag
     4321 cgcaccggtc acaatcagca ggtcgttgct gagcataaag cccgcagccg cagccgccca
     4381 gccggagtac gagttcagca tcgacaccac cactggcata tctgcaccac cgatggaggc
     4441 gactaaatgc cagccgaata ccagcgcaat ggcggtcatt attagcaatg ccagcacttg
     4501 caggccgacg ctgtccgtgc gaacaaatac aatcagcagc aggaaggaaa cgaccagagc
     4561 cgccaggttc attttgtgac ggtttggcag catcaacggt ttagacgaaa tcttgccaca
     4621 cagtttgccg aacgccacca ccgaacccgt gaacgttacc gccccgatga agataccgag
     4681 gaacacttcc gtcaggtgaa tattgaccag aatcggtgcc attcccgcgt catgatgcag
     4741 atagctgtta aagccaacca gcactgccgc cagacccacg aagctgtgca ggatcgccac
     4801 cagttctggc atttcggtca tttcaacttt cttcgccaga cggataccaa ttgccccacc
     4861 aatgaccatc gccagcaaga tccagccaac attacccgta tccggtccaa aaatggttgc
     4921 gattaacgca atcgccatcc cggcgatacc gaagttgtta ccctggcgag acgtttcatg
     4981 tttcgaaaga ccggccagac tgaagataaa caggatcgcg gcaacaatgt atgcagctgt
     5041 aactaatcct ccagacatat gttacccctt aatttttgcg gaacattttc agcatgcgct
     5101 gagtcacggt gaagccaccg aaaatattaa tgctggctat aagcaccgcg ataaaactaa
     5161 ggaagctaac ccagccgccc tggccaatct gcaacagtgc tccgacaaca ataatccctg
     5221 aaatcgcgtt ggtgaccgac atcaacggtg tatgcagcgc gtgcgataca ttccacacca
     5281 cgtaataacc gacaacgcag gccagcgcga aaacggtgaa gtgcccaaga aactctttcg
     5341 gcgcaacgct tgccagccaa ccgaaaagga tgattgccag cgccatcaac gcgtatttac
     5401 gccacggtga gcaggcacat ttttcctcag ttttcacttc cggtgccgct ttttgtgccg
     5461 cctgcggctg agctgatacc tgaatcggcg gtgccggcca ggtaatttcg cccgcacgga
     5521 tcacggtcac gccgcgaatc accacatcat caaaatcaac agtgatattg ccgtctttct
     5581 ctttgcacaa cagtttcagc agattaacga ggttagtacc gtaaagctgc gaggattgcg
     5641 tcggcagacg gcccggaaga tcggtataac caatcacttt gacaccattt tccgtagtga
     5701 agatttcacc cggcacggtg tattcacagt tgccaccgtt ttgggctgcc aggtcgacaa
     5761 tcacactgcc cgccttcatg gagtcaacca tttcacgggt aattagcttc ggcgctggtt
     5821 tgcctggaat aagcgcggtg gtgacaatga tatcgacctc ttttgcctgg gcggcaaaga
     5881 gttccatttc cgctttaatg aacgcgtccg acatcacttt ggcatagcca tcgccactgc
     5941 ccgcttcctc tttaaaatcc agctcgagga attccgcgcc catactttga acttgttctt
     6001 tcacttccgg acgggtgtcg aatgcacgca caatcgcgcc gagactgttt gctgcgccaa
     6061 tggcggccag acctgcaaca cccgcaccaa tcaccatcac ttttgccggt ggcactttcc
     6121 cggccgcagt aatttgcccg gtaaagaagc gcccaaattc atgtgccgct tcaacaatgg
     6181 cgcgataacc ggcgatgttc gccatcgagc ttagtgcgtc cagcgattgt gcgcgtgaga
     6241 tacgcggcac agaatccatc gccatcacgg tcacgttacg ttccgcaagt ttttgcatta
     6301 attccggatt ctgcgcaggc cagataaaac tcaccagcgt tgtcccagga ttcagtaacg
     6361 caatttcatc atctaacggc gcattgacct tcagaatgat ctctgactgc cagacgctat
     6421 tcccttctac aatttcagcg cccgcttgca caaacgcttt atcgtcaaaa cttgccagtt
     6481 gacccgcgcc gctctctacc gcgacggtaa aacccagttt cagcagctgt tccactgttt
     6541 ttggcgttgc tgcaacacgg gtttcattgg ttaaccgttc tcttggtatg ccaattcgca
     6601 tgatattccc ttccatcggt tttattgatg atggtttgcc tgtgtcagga gccacacaag
     6661 ccgctcatgt acgagctaaa tgttactccg ttaaaataaa ttagtaacaa acgccctata
     6721 acgtactgaa aattatgcct gtgatctagc gccaaaaatc agtatttcgg cgtgaactcg
     6781 caaaatatta acgattcagc cgtgatagtg ggataaacac cttagaacgc cggataaaga
     6841 ctgataattg tcttcgacgg ccgggtaaaa cgagacatcg ccccggcacg attcactact
     6901 taacattaaa ttaacttata caattcagtt gcttcagtag taatgatgct gatacggctg
     6961 ttttttaagc atagacggtc atttgagcag gattaaaatt ggcttaagga atgtgatatg
     7021 aaaaatgacg cagacagtta caccgtttaa atgcaataat cagccacgtt tctcgttaat
     7081 aacaatacca gtacctggtt tgcgcaaggc gaaggattat ttttatgaag cttaagaaca
     7141 ccctcctggc gtcggcactg ctttctgcta cggcattctc cgttaacgca gcaacagaac
     7201 tgacaccgga gcaagcggca gcggttaaac cttttgaccg tgtagtggtt accggtcgtt
     7261 ttaatgctat tggcgaagcg gtgaaagccg tttctcgtcg cgcagataaa gaaggtgccg
     7321 cctcttttta tgttgtcgac acttctgatt ttggtaacag cggtaactgg cgtgtggtcg
     7381 ctgacctcta taaagccgat gctgaaaaag cagaagaaac aagtaatcgc gtaattaacg
     7441 gtgttgtcga actgccgaaa gatcaggctg ttctgattga accgtttgac acggtcaccg
     7501 tccagggctt ctatcgtagc cagccagaag tcaatgatgc catcaccaaa gcggcaaaag
     7561 cgaaaggtgc ctactctttc tacatcgttc gtcaaatcga tgccaaccaa ggcggcaacc
     7621 agcgtattac tgcattcatc tataaaaaag atgctaagaa acgtatcgtc cagagcccgg
     7681 atgtgatccc ggcagattcc gaagcaggac gtgcagctct ggctgccggt ggcgaagccg
     7741 cgaagaaagt tgagatccta ggtgttgcga ctaccgcatc accaagttct gaagtcggtc
     7801 gtttctttga aacccagtca tcaaaaggcg ggcgttacac cgtcatgctc ccggatggca
     7861 ctaaagtcga agaactgaac aaagcgaccg cagcgatgat ggtcccgttc gacagcatca
     7921 aattctctgg caactacggc aacatgaccg aagtctccta tcaggttgcg aaacgtgccg
     7981 cgaagaaagg tgctaagtat taccacatca cccgccagtg gcaggaacgt ggtaataacc
     8041 tgaccgtcag cgcagatctg tataaataac tattcagtta ctcctataag gcggcttgat
     8101 gccgcctttc gcatttttag tcacaaattt ccccgcgacc attgcatgca gacacatctc
     8161 ttcgtaaaat cccgcgcttc agtggcaaac tccattttta tgcgttttgc cctaatattt
     8221 attcttctta tcacgtttta atcactggat atcgatgaaa aagaaactgg gactgagcgc
     8281 actcaccgcg ctggtattaa gctcaatgct gggcgcgggt gttttcagtc tgccgcaaaa
     8341 tatggcggca gttgccagcc cggcagcact gctcatcggc tggggtatta ctggcgctgg
     8401 cattttattg ctggcctttg ccatgctgat cctcacgcgc attcgtcccg aacttgacgg
     8461 cggtatcttt acctatgccc gcgaagggtt tggcgagcta atcggctttt gttccgcatg
     8521 gggatactgg ctgtgcgcag tcatcgccaa cgtctcctat ctggtgatcg ttttttccgc
     8581 gttaagcttt tttacggaca cgccggaatt gcgcctgttc ggcgatggta atacctggca
     8641 atcgatcgtc ggtgcatcgg ctttattgtg gattgttcac tttttgatcc tgcgcggggt
     8701 gcaaaccgct gccagcatta acctggtggc gacactggca aaattgttgc cgctgggtct
     8761 gtttgttgtg ctggcgatga tgatgttcaa actggatacc ttcaagctcg acttcaccgg
     8821 acttgcactt ggcgtacccg tttgggaaca ggtgaaaaac accatgctga tcaccctgtg
     8881 ggttttcatt ggtgtggaag gtgccgtggt tgtttctgcg cgtgcgcgta ataaacgtga
     8941 tgttggcaaa gcgacactgc tggcggttct ctccgctctg ggcgtttact tgctggtaac
     9001 gctgctttcg ctgggtgtgg tggcacgtcc tgaactggct gaaattcgta acccgtcaat
     9061 ggccggtctg atggtggaaa tgatgggacc atggggcgaa atcatcatcg ctgccggttt
     9121 gatcgtttcc gtttgcggcg cgtacttaag ctggaccatc atggcggcag aagttccgtt
     9181 cctggctgcc actcataaag cattcccacg catttttgcg cgccagaacg cacaagctgc
     9241 gccatccgcc tcactgtggt tgaccaacat ctgcgtgcaa atttgtctgg tactcatctg
     9301 gctgaccggt tccgattaca acacgttgct gaccatcgcc tcagaaatga ttctggtgcc
     9361 ctatttcctg gtcggcgcgt tcttgctgaa aatcgcaacc cgtccactgc ataaagcggt
     9421 aggtgtcggt gcctgcattt atggcttatg gttattgtac gcctctggtc caatgcactt
     9481 gctgttatcc gttgttctgt atgcgccggg gctgctggtt ttcctgtatg cgcgcaaaac
     9541 gcatacacat gataatgtac tgaaccgtca ggaaatggtg ctgattggca tgctgttaat
     9601 tgcctcagtt ccggcgacct ggatgctggt gggataacgt tcatcccatc gttggatagc
     9661 aaaggagata acgatgggta aagcccagcc cttgccaata ttaattactg gcggaggtcg
     9721 tcgcatcggc ctcgccctcg cctggcattt cattaatcaa aagcaaccgg tgattgtcag
     9781 ctatcggacg cactatccag ccattgatgg actgattaag gcaggtgcgc agtgtattca
     9841 ggctgatttt tcgaccaacg acggtgtgat ggcgtttgcc gatgaagtac taaaaagccc
     9901 ccatggtctg cgtgctattt tgcataacgc cagtgcgtgg atggcggaaa aaccgggtgc
     9961 gccactggcc gacgtactgg cttgcatgat gcagatccac gttaataccc catacctgct
    10021 caaccatgcg ctggaaagat tactgcgtgg gcacggacac gccgccagcg atatcattca
    10081 ctttaccgat tatgtggtgg agcgcggtag cgacaagcat attgcgtatg ctgcaagcaa
    10141 agcggcactg gataatatga cccgctcgtt tgcccgcaag ctggcaccgg aagtgaaagt
    10201 gaactctatt gctccatcgc tgatcctgtt taatgaacat gatgatgccg aatatcgaca
    10261 acaggcgctg aataaatcac tgatgaaaac cgcgcctggc gagaaagaag tgatcgacct
    10321 ggtcgattac ttacttacca gttgctttgt caccggacgc agtttcccac ttgatggcgg
    10381 tcgtcatctg cgttaatgca gctttatcca gcatataatc aacacccatg cgcttatccc
    10441 ccaacacgaa acagaaccaa caaaagcggc gggcaagcgc atcattccgg tgaaatacca
    10501 cagcgacacc aggtaaacaa aatagggaat aatcgaccac atactaaaaa tgatggttgc
    10561 gcgtaaggct tcaatgccgc gttcgctggc aacaatataa tgcgcgataa gcgcaaaggt
    10621 cgggaaaagt ggaatcagcc cggcgatata ataatttttc gtttttgcta aaacaccaat
    10681 caacagcact accagcgcac cgagcgcggc tttgattacc agccccattt ttttacctta
    10741 acacttccat aacaagtcat cagtagaata cctgatgaaa acttgtttag aaacgattga
    10801 tagtaagtaa aaacagcgcg gtgtattgtg acgtttttat atctaccgtg aatgttatga
    10861 acactatcgt atttgtggaa gatgatgcgg aagtcggttc actgattgcc gcgtacctgg
    10921 caaaacatga tatgcaggtt accgtagagc cgcgcggcga ccaggccgaa gaaaccattt
    10981 tgcgagaaaa tccggatttg gtgttactcg acatcatgct accaggcaag gacggcatga
    11041 ccatttgtcg tgatttacgc gcaaagtggt ctggaccgat tgttcttcta acctctctcg
    11101 atagcgatat gaaccacatc ctggcactgg aaatgggtgc ctgcgactat attctcaaaa
    11161 cgacgccccc tgctgttttg ctagcgcgtt tacgtttgca tttgcgtcag aatgagcaag
    11221 ccacactgac caaaggtctt caggaaacgt ctctgactcc ctacaaagcc ctgcatttcg
    11281 gcacgttgac catcgatccc atcaaccgcg tagtcaccct ggctaacact gaaatctcgc
    11341 tctcgacagc tgatttcgaa ttattgtggg aattagctac ccatgccggg caaatcatgg
    11401 accgcgatgc attgctgaaa aatttacgcg gcgtcagtta tgacggactg gatcgtagcg
    11461 tggacgtggc tatttcgcgg ttaagaaaaa aactgctcga taacgccgca gaaccttatc
    11521 gcattaaaac tgtgcgtaac aaaggctatc tttttgcgcc tcatgcatgg gaataagcga
    11581 tgaaaaaact gtttatccag ttttacctgt tattgtttgt ctgcttcctt gtgatgtctc
    11641 tgctggttgg gctggtgtac aaatttaccg ccgaacgcgc gggcaaacag tcgctggatg
    11701 atttgatgaa cagttcactg tatctgatgc gcagcgaatt gcgtgagatc cccccacacg
    11761 actggggtaa gacgctgaaa gagatggatt taaatctctc tttcgatctg cgtgtcgagc
    11821 cactgagtaa ataccatctt gatgatattt ccatgcaccg actgcgtggc ggcgaaattg
    11881 tcgccctgga cgatcagtac acgtttttgc agcgtatccc gcgcagccac tacgtgctgg
    11941 cagttggtcc tgttccttat ctttattatc tccatcagat gcgattgctg gatatcgccc
    12001 tgatcacttt tattgctatt tccctcgcct ttccggtgtt tatctggatg cgtccgcact
    12061 ggcaggatat gttaaaactg gaagcagcgg cgcaacgatt tggcgatggg catctcaatg
    12121 aacgtatcca ctttgatgag ggttcgagct ttgaacgact tggtatcgca tttaaccaga
    12181 tggcggacaa tatcaacgcc ttgattgcca gcaaaaaaca gcttattgac ggtatcgctc
    12241 acgaactgcg aacaccgtta gtgcgcctgc gttatcgact ggagatgagc gataacctga
    12301 gcgccgccga atcccaggcg ttgaatcgtg atatcagtca acttgaagct ttaattgaag
    12361 agctgctgac ttatgcccga ctcgatcgcc cacaaaacga gcttcatctt agcgaaccag
    12421 acctgccgtt gtggctgtca acgcatctgg cagacattca ggcagtaacg cccgataaaa
    12481 cggtacggat taaaacgctc gcgcaaggcc attatgcggc gttggatatg cgcttaatgg
    12541 agcgcgtgct ggataatttg ctcaataacg ccctgcggta ctgccattca acggttgaaa
    12601 ccagcctgct actgtcgggg aatagagcga cattaattgt tgaggatgat ggcccaggga
    12661 ttgccccaga aaaccgcgaa catatctttg aaccttttgt tcgcctcgat cccagccggg
    12721 atcgctcaac cggcggctgc gggctggggc tggcaattgt ccactctata gcactggcaa
    12781 tgggcggtac ggttaattgt gacaccagcg aactgggtgg tgctcgcttc tcgtttagct
    12841 ggccgttatg gcataacatc ccgcaattta cctctgcctg acactacgcg cacgatggtc
    12901 aagtcaccac cactgtgcta taaaataagt atgttgtaac taaagtggtt aatattatgg
    12961 cgcgttacga tctcgtagac cgactcaaca ctacctttcg ccagatggaa caagagctgg
    13021 ctgcatttgc cgctcatctt gagcaacaca agctattggt tgcccgcgtg ttctctttgc
    13081 cggaggtaaa aaaagaggat gagcataatc cgcttaatcg tattgaggta aaacaacatc
    13141 tcggcaacga cgcgcagtcg ctggcgttgc gtcatttccg ccatttattt attcaacaac
    13201 agtccgaaaa tcgcagcagc aaggccgctg tccgtctgcc tggcgtgttg tgttaccagg
    13261 tcgataacct ttcgcaagca gcgttggtca gtcatattca gcacatcaat aaactcaaga
    13321 ccacgttcga gcatatcgtc acggttgaat cagaactccc caccgcggca cgttttgaat
    13381 gggtgcatcg tcatttgccg gggctgatca cccttaatgc ttaccgcacg ctcaccgttc
    13441 tgcacgaccc cgccacttta cgctttggtt gggctaataa acatatcatt aagaatttgc
    13501 atcgtgatga agtcctggca cagctggaaa aaagcctgaa atcaccacgc agtgtcgcac
    13561 cgtggacgcg cgaggagtgg caaagaaaac tggagcgaga gtatcaggat atcgctgccc
    13621 tgccacagaa cgcgaagtta aaaatcaaac gtccggtgaa ggtgcagccg attgcccgcg
    13681 tctggtacaa aggagatcaa aaacaagtcc aacacgcctg ccctacaccg ctgattgcac
    13741 tgattaatcg ggataatggc gcgggcgtgc cggacgttgg tgagttgtta aattacgatg
    13801 ccgacaatgt gcagcaccgt tataaaccac aggcgcagcc gcttcgtttg atcattccac
    13861 ggctgcacct gtatgttgcg gattaacgcc cggctttcat actgccgacc atctgttctg
    13921 gccgtaccca gctgtcaaac tcggcttcgc taagataccc cagcgcaagg gccgcagctt
    13981 ttaaggtcag cccttcttta tgcgcttttt tggcgatctc cgcggctttg tcataaccaa
    14041 tgtgggtgtt aagcgcagtc accagcatca gcgattcatt gagtaattga ttgattcgct
    14101 cacgattcgg ttcaataccc actgcgcagt gtttgttaaa actttccatg ccatctgcca
    14161 gcaagcgcac cgattgcagg aaattgtgga tcaccattgg acggaagacg ttcagttcaa
    14221 agttaccgga agcgccaccc atgttgatcg ccacgtcgtt ccccattacc tgacagcaga
    14281 gcatggttaa tgcttcacac tgggttgggt tcactttccc cggcatgatt gagctgcccg
    14341 gctcattttc cgggattgag atttcaccaa ttccgcagcg cgggccagag gccagccagc
    14401 ggacatcatt ggcgattttc atcagtgacg cagccaaccc tttcaacgcg ccgtgcgcct
    14461 gaaccagggc atcacaggtc gcaagcgctt caaatttgtt cggcgcggta acaaacggtg
    14521 cacaggtaat gactgccagt tcatctgcta cgcgacgcgc atactccgga tgggtattta
    14581 gtccagtacc caccgctgta ccgccgagag ccagttccgc tacgtgaggc aggctgtatt
    14641 cgatatgttt gagattatgc tcgagcatcg ctacccagcc ggaaatctcc tgccccagtg
    14701 ttagcggcgt ggcgtcctgc aagtgggttc gaccgatttt gacgatatcg gcaaatgcac
    14761 gcgatttttc actcagtgtc tgtgtcaggg ttttaacctg cggaatgagt tgcttgcgca
    14821 gcgccagcag cgccgcaacg tgcatcgccg tcggaaagac atcgttggaa ctttggcttt
    14881 tgttcacgtc gtcgttaggg tgaactttac gttccatccc gcgcacgccg ccgagtaatt
    14941 cactggcccg gttagccagc acttcgttca tgttcatgtt actttgcgtg ccggagccgg
    15001 tctgccagat agccagcggg aattcgtcgt catgctgtcc tgccagtact tcatccgccg
    15061 cctgccgaat ggcgctcgct ttctcttcag acaacaagcc taaatcttca ttaacttttg
    15121 ccgctgcgcg cttggttagc gccagcgcat gaatcagtga ggtgggcatt ttctccgtcg
    15181 aaatgcggaa atgctccagc gagcgttgag tttgtgcgcc ccacagctta tctgccggga
    15241 catcaatcgc ccccatcgaa tctttttcgc tgcgtactgt attcatggcc tgctcctcac
    15301 ctgattaatt ttttctttct gttttgcctt cgttaagcaa cttttagctc acttattatt
    15361 tactatttga taacaaatgt ttggtctttc gtgccatgta aaaaaaccgc cccgaagggc
    15421 ggctctgttt atttcacaca gcgagtgcac tgtgtgagtt gtatctgctg gaagaagtca
    15481 tttcctttat catccacaag gataaacgcc gggaaatctt ccacttcaat tttccagatg
    15541 gcttccattc ccagttccgg atattcaaca cattccaggc tcttaatact tccctgcgcc
    15601 aatacagcgg ccggaccacc gatactgcca aggtagaagc cgccgtgttt tttacaggca
    15661 tccgtcacct gctggctgcg gttgcctttc gccagcatga tcatacttcc gccctgcgct
    15721 tgcagttgat cgacataaga atccatccgt ccggcggtcg ttgggccaag agaaccggag
    15781 gcataacctt ccggcgtttt ggccggaccc gcgtagtaaa tcggatgatc tttaatgtac
    15841 tgcggcagcc cttcaccgtt atccatccgc tctttcagtt tggcgtgagc aatatcacga
    15901 ccgacgataa tcgtgccgtt aagcgataag cgtgtagaaa cgggatactg cgacaactgt
    15961 gcgaggatct ctttcatcgg acggttaagg tcaacgcgca ccgcttcgcc ttctcccgct
    16021 ttacgcagct cttcagggat atatttacct ggattatgtt ccagtttttc gatccagatc
    16081 ccctgacggt tgatcttcgc tttgatatta cggtcagcag agcaggaaac acccatacca
    16141 accggacagg atgcgccgtg acgtggcagg cgaatcacgc gtatgtcgtg agcgaagtat
    16201 ttaccaccaa actgcgcacc cagaccgaga ttttgcgctt cgatcagcaa ttctttttcc
    16261 agttccacat cgcggaatgc ctgaccgtgc tcattccctt ccgttggcag ttcgtcatag
    16321 tatttagcgg aagccagttt cactgtttta aggttagttt ctgcagaagt tccaccaatt
    16381 acgaacgcaa tatgatacgg aggacaggcc gccgtaccca gcgtgcgcat cttctcaacc
    16441 aggtaatttt tcagtttccc cggcgttagt aacgctttgg tttcctgata gagatacgtc
    16501 ttgtttgccg aaccaccacc tttggcgata cagaggaatt tgtactcgtc gccatcaacg
    16561 gcataaagat cgatctgcgc tggcagattg gtgccggtat tcacctcttt atacatatcc
    16621 agcggcgcgt tttgcgagta gcgcaaatta tcttcgatat aagtgttata gacaccgcgc
    16681 gccagcgccg cttcatcaca gccaccggtc catacacgct gccctttttt accaacaata
    16741 atcgcagtgc cggtatcctg acaggttggc agaacacctt tcgccgcgat gtcggagttg
    16801 cgcaggaatt gcagcgccac atatttatca ttttcgctgg cttccgggtc acgcagaatg
    16861 tcggccacct gttgttggtg agccggacgc agcataaatg acgcatcatg aaacgcctga
    16921 cgcgccaaca gagttaacgc ttcgggtgcg actttcaaaa tctcctgccc ttcaaattca
    16981 gatacgctaa cgtgttcgct ggttagcagg taatactcag tatcatcttt tttgagtgga
    17041 aaaggagcct gataatgaaa gggtttgttt gacattgttc tctcacttac tgcctggttt
    17101 ggttatgctc tgggcgggtg ttccgttgcc ctgttaaaag cgagtaacaa tatcctacac
    17161 acttttttaa caaaaactga gactagtacg actttttgcg gctccaggtt acttcccgta
    17221 ggattcttgc tttaatagtg ggattaattt ccacattaaa acagggattg atcatgcaaa
    17281 aactcattaa ctcagtgcaa aactatgcct ggggcagcaa aacggcgttg actgaacttt
    17341 atggtatgga aaatccgtcc agccagccga tggccgagct gtggatgggc gcacatccga
    17401 aaagcagttc acgagtgcag aatgccgccg gagatatcgt ttcactgcgt gatgtgattg
    17461 agagtgataa atcgactctg ctcggagagg ccgttgccaa acgctttggc gaactgcctt
    17521 tcctgttcaa agtattatgc gcagcacagc cgctctccat tcaggttcat ccaaacaaac
    17581 gcaattctga aatcggtttt gccaaagaaa atgccgcagg tatcccgatg gatgccgccg
    17641 agcgtaacta taaagatcct aaccacaagc cggagctggt ttttgcgctg acgcctttcc
    17701 ttgcgatgaa cgcgtttcgt gagttttccg agattgtctc cctactccag ccagtcgcag
    17761 gtgcacatcc ggcaattgct cactttttac aacagcctaa tgccgaacgt ttaagcgaac
    17821 tgttcgccag cctgttgaat atgcagggtg aagaaaaatc ccgcgcgctg gcgattttaa
    17881 aatcggcgct cgacagtcag cagggtgaac cgtggcaaac gattcgttta atttctgaat
    17941 tttacccgga agacagtggc ctgttctccc cgctgttact gaatgtggtg aaattgaatc
    18001 ctggcgaagc gatgttcctg ttcgctgaaa caccgcacgc ttacctgcaa ggcgtggcgc
    18061 tggaagtgat ggcgaactcc gataacgtgc tgcgtgcggg tctgacgcct aagtacattg
    18121 atattccgga actggttgcc aatgtgaaat tcgaagccaa accggctaac cagttgttga
    18181 cccagccggt gaaacaaggt gcagaactgg acttcccgat tccagtggat gattttgcct
    18241 tctcgctgca tgaccttagt gataaagaaa ccaccattag ccagcagagt gccgccattt
    18301 tgttctgcgt cgaaggcgat gcaacgttgt ggaaaggttc tcagcagtta cagcttaaac
    18361 cgggtgaatc agcgtttatt gccgccaacg aatcaccggt gactgtcaaa ggccacggcc
    18421 gtttagcgcg tgtttacaac aagctgtaag agcttactga aaaaattaac atctcttgct
    18481 aagcttatta aaggcttata acaccttcag gcggccagtc cgcctgattt cattttatgg
    18541 ataatcatta tgaataaatc gctggtagcg gtaggcgtca ttgttgcgct aggcgtagtc
    18601 tggacaggcg gcgcatggta tacaggcaag aagattgaaa cccatctcga agacatggtc
    18661 gcgcaggcga acgcgcaact caaactgaca gctcctgaat ccaacctgga agtgagttat
    18721 caaaactatc atcgcggcgt attcagcagc cagttgcaac tgttggtgaa acccattgcc
    18781 gggaaagaaa atccgtggat taaaagcggt cagagcgtca tcttcaacga atcggttgat
    18841 catggtccct tcccgcttgc ccagcttaaa aaactgaacc tgatcccgtc gatggcatca
    18901 attcaaacca cgctggttaa taacgaagta agcaaaccac tgtttgatat ggcaaaaggt
    18961 gaaacgcctt ttgagattaa ctcgcgcatt ggttacagcg gtgattccag ttccgatatt
    19021 tcgctcaagc cactgaatta cgagcaaaag gatgaaaaag tcgcctttag cggcggcgag
    19081 ttccagttaa atgctgacag agacggcaaa gccatctccc tttccgggga ggcgcaaagt
    19141 ggtcggatag acgcagttaa cgaatacaac cagaaagtgc agttgacctt taataatctg
    19201 aaaaccgacg gttccagcac gctggcaagt tttggtgagc gcgtaggaaa ccaaaaactg
    19261 tcactggaaa aaatgaccat ttcagtggaa ggcaaagaac tggcactgct ggaaggcatg
    19321 gagatcagcg gtaaatcgga tctggtcaat gacggtaaaa cgatcaatag ccaactggat
    19381 tactcgctaa acagcctgaa ggtacagaat caggatctgg gcagcggcaa gctgacttta
    19441 aaagtcggcc agattgatgg tgaagcctgg catcagttta gccagcaata taacgcgcaa
    19501 actcaggcgc tgctggcaca gccagaaatt gccaacaatc ccgaacttta tcaggagaaa
    19561 gtgacggaag ccttctttag cgccctgccg ctgatgttga aaggcgatcc ggtgattact
    19621 atcgcgccgc taagctggaa aaacagtcag ggtgaaagtg cgctgaatct gtcgctgttc
    19681 ctgaaagatc cggcaacgac taaagaagcg ccgcaaacgc tggcgcagga agtagatcgt
    19741 tcggttaaat ctctggatgc gaaactgacc attccggtgg atatggcaac tgagtttatg
    19801 actcaggtag cgaagctgga aggttatcag gaagatcaag cgaaaaaact ggcgaaacag
    19861 caagttgaag gtgcatcagc aatggggcag atgttccgtc tgaccacctt gcaggacaat
    19921 accatcacca ccagcctgca atatactaac ggtcagataa cgttaaacgg gcagaaaatg
    19981 ccactggaag atttcgttgg tatgtttgcg atgccagctc ttaacgttcc ggctgtaccg
    20041 gcaattccgc agcagtaatt cacggccaaa acgccggaga attttcactt cggcgttttc
    20101 atctgaaaac aaaccagaga ttgcctgatg cgctacgctt atcaggccta ccttttcctg
    20161 caacactttg aatttatgag tttttgtagg ctggataagg cgtttacacc gcatccggca
    20221 taaaaaacgc gcactttgtc aacaatctga aacgccggag gtttttctct ccggcgttat
    20281 ttctgacttc agcataaagt catactgaat actcattttc acatcccagt aattcaggcg
    20341 atcgcccagc gtactgccat cgcgcttttc ccgcccttta tcggcagcaa gaaagaaatt
    20401 catcccttgt aacggccctt cgctgacccg atagttcata ccacctaaga tacccgcgcc
    20461 ttcaatccgc acatctcgtt tactggattc atagccgcga gtcacaatct gtgcaaatgt
    20521 cagtgtaaat tgcggcgtta aacgatagtt gacgcccaat tgccaggact gcattccttc
    20581 acggttacga tcaagactta atgaaaaggg atagccaatg tcggtatcca ccacggcacc
    20641 attcattgca tggcgcaaat aaccaccact gccaaatacg cttccccacg gcgcattcca
    20701 ggtcaattgt ccactaacca acgccgtttc attgggctgg ctggtattac gacttaaacc
    20761 ttcgagctgc gcgtaaaagc cgagcaactc ccctttcaac gtataattcg ggtcgagcgg
    20821 tatgcttttc tcgagaatac tttgtatctg attggaaaca tctttctgta ccgatgcccc
    20881 cagagaatag cgactggtat catcatgcca actggcggct aaaaaagaac gtgcgcgatg
    20941 tttatcgtat tttcccgtct ggctgttata caccccttca tcacgaatac cggttgttga
    21001 atcagtccat aaaaatactc gtgaacgcca cattccccac agatcaatat ttggtgttaa
    21061 agcccaataa cctaatgctc cttcagaaac tgtcggtaat gtacgttgcg aactgccagc
    21121 cattaacgga atacgataag gtaagctacc ataactataa tccgtcccga agcgacctga
    21181 tttaagcagg ctattttcac tgggttttat ttttactgcc cccagggcaa agccaaaact
    21241 tttatcacca tcaagatacc accgggtact catatcagca cgagcaccta atttataaac
    21301 ataatatgcc ccgccttcaa cgccaataat attggaataa taaccactat tgaaatcgag
    21361 caatcctccc tgtacccatg cgtaaatatc tcttcccgcg ccagcactgg gcttatccgc
    21421 cctgcgcaat tcgtttttca gtctgatgcg tagtcccgcc tcatcgtcag ccagttgcgc
    21481 cgcataagca gagataaggc cagaggcagc cgtcaggcaa ataacggcca tggccactat
    21541 ttttctcata gttgcacctt tgatgttctg attattttta ttgattatta attagtgata
    21601 tcgctgatta attgctgctg cactttttta cgattatcaa tttcaaccac gatttctttg
    21661 aattttttat ccgtaagcgg ataaaaccag ataataacga atgccagtag cataaatccg
    21721 caaggtacta aggcaattga tgtgcggatg cccataataa cttccggcgt ttgcacctga
    21781 ttggcaatat atccgcttaa ccccaaaata aaggcaggaa ttgaacctcc gattgcctga
    21841 ccgcatttac gggtaaatga gaatagtgaa taggtgagcc cttcaattcg cacgccggtc
    21901 aggtattcac cgtattctac ggtatcagct tccagtgccc acatcactgt catagtaacg
    21961 ccctgaccaa ttgaagcgat ggccaacgca accaacgcca ccggcagtga ccagacggaa
    22021 acccagaaga atagcagata accgcaggtt cccagcaaag cgccaatcag gaaggtattc
    22081 tttttaccga tcctcgcgac catccccggc acaagcggtg ccgatgccac agtaccaacc
    22141 aggttttgca ccagtaccag cacagtgaac agcccggtat catttaacac atagcgcacg
    22201 tagaacaacg acgaggcgct gaccgcaaag gtcgaaatca gcacacacag cgcaccgatg
    22261 cacaacataa acagcgggcg attccgtttc agggtttgca aactgatctt caatgacggc
    22321 tgcgcaacga tacgtaccac attctcacgc gtcgatttga agcagatgaa gtaaagcacc
    22381 attccggcaa tcgccagcac aatcgtccag aaatggtata ccgacaccat ctcttccggg
    22441 ctggagttct taatgctcgg ccctatcaga aatgccaggc agacaaaggt caatgaagcg
    22501 gcaatcccac gagccgcgcc cagacgggcg cgggattgtg gttgttgggt catcgcggta
    22561 gcaagtgaac cataaggaat attcaccagg ctgtagcaaa gcccgaggcc catgtaggtc
    22621 aaatatgcat acactacttt gcggctatgg ctccagtcgg tcggtaccca gaataccagc
    22681 acgctgaaga tcattaacgg cgcagtaccg aagagtaaaa acgggcggaa ttttccccag
    22741 cgggtattca cactgtccac cactcgtccg gcaaagacgt cggcgaaggc atcgaatacc
    22801 cgcaccagta acagcatggt gcccgccgca gcggcaccga cgccagcgac gtcggtgtag
    22861 taactcaaca ggaagagcac ccccattgcg aaggcgaagt tattggcgac gtcaccgagg
    22921 ctgtagccga cgatggtgcg ccaggagagt tgttgattca ttgtttgcct ccctgctgcg
    22981 gtttttcacc gaagttcatg ccagtccagc gtttttgcag cagaaaagcc gccgacttcg
    23041 gtttgcggtc gcgggtgaag atccccttct tgttaccgcc aacgcgcaat atgccttgcg
    23101 aggtcgcaaa atcggcgaaa ttccatacct gttcaccgac gacggcgctg acgcgatcaa
    23161 agacgcggtg atacatatcc agccatgcac actgatactc ttcactccac atgtcggtgt
    23221 acattgagtg cagcccggct aacgtatcca cgccgtattc ggtgatgata atcggctgat
    23281 gcagtttctc ctgccaggcc agaagttctt tttccagtac cttctctgcc gtttccaaat
    23341 cgccgctttg gacataccat ccgtaataac ggttcaggca cagcacatca aagagatcgc
    23401 tgatggtatc ggtgtgagcg tcgcagaaca ttacattgac gcaggtgatc ggacgcgtcg
    23461 ggtcgagttt acgcgttgct tccgccagtg gcgcgaaata ttcccgtgca ccttgcggac
    23521 gggtatccgg ttcgttggca atactccaca tcaccacgct tgggtggttt ttgtcacgcg
    23581 ctatcagctc tttaatcgcc tgtaagtgcg cttgctgagt ttccccgttg actgcctctt
    23641 cgctgtacag ttctttcggc ttgttgcccg cttcgaaacc aatgcctaaa gagaggttaa
    23701 agccgacagc agcagtttca tcaatcacca cgatgccatg ttcatctgcc cagtcgagca
    23761 tctcttcagc gtaagggtaa tgcgaggtac ggtaggagtt ggccccaatc cagtccatta
    23821 atgcgtggtc gtgcaccatc agcacgttat cgaatccttt gccacgcaag tccgcatctt
    23881 catgacgacc aaagccagta aagtagaacg gtttgtggtt aatcaggaac tgttcgccct
    23941 tcactgccac tgaccggatg ccgacgcgaa gcgggtagat atcacactct gtctggcttt
    24001 tggctgtgac gcacagttca tagagataac cttcacccgg ttgccagagg tgcggattca
    24061 ccacttgcaa agtcccgcta gtgccttgtc cagttgcaac cacctgttga tccgcatcac
    24121 gcagttcaac gctgacatca ccattggcca ccacctgcca gtcaacagac gcgtggttac
    24181 agtcttgcgc gacatgcgtc accacggtga tatcgtccac ccaggtgttc ggcgtggtgt
    24241 agagcattac gctgcgatgg atcccggcat agttaaagaa atcatggaag taagactgct
    24301 ttttcttgcc gttttcgtcg gtaatcacca ttcccggcgg gatagtctgc cagttcagtt
    24361 cgttgttcac acaaacggtg atacgtacac ttttcccggc aataacatac ggcgtgacat
    24421 cggcttcaaa tggcgtatag ccgccctgat gctccatcac ttcctgatta ttgacccaca
    24481 ctttgccgta atgagtgacc gcatcgaaac gcagcacgat acgctggcct gcccaacctt
    24541 tcggtataaa gacttcgcgc tgataccaga cgttgcccgc ataattacga atatctgcat
    24601 cggcgaactg atcgttaaaa ctgcctggca cagcaattgc ccggctttct tgtaacgcgc
    24661 tttcccacca acgctgatca attccacagt tttcgcgatc cagactgaat gcccacaggc
    24721 cgtcgagttt tttgatttca cgggttgggg tttctacagg acgtaacata agggactcct
    24781 cattaagata aaaatactgg tcaaccttta atctgattag ttaaccaatt ctgtgttgca
    24841 cacagttatc tgtgagaaac ccatggaaga gaaatggaaa taaaaagaac tgacaccgcg
    24901 atcaaaaaac caaaaactgc aatacccaca gcgtgatatt gatcgcatta atctttaaaa
    24961 cggcgttgac agaagccata aaataagcaa ccgaacgttc gttcgagcat attgggaacg
    25021 ataaactctc atgacatatg gctacggtga atatttttgg gggggaattc tccgatcccg
    25081 gctagattgt attttgtaca gggaaattaa agtatgaaac gcgggcacag ttatcaccag
    25141 cccgcgaaat gagaggatca ggatgcggtt aagataccgc caatcatcgc gcgtaatccc
    25201 tgagcaaacg acgcttcacg cgccagatct tccgcttcga tatcggccag tacaccgtag
    25261 gtcagatcca gcagtaaacg tgaagtcatt gacgtgttga tgtctgggct tatctcgcct
    25321 ttttgctggg catcacgcat ccgctgggca acaaattccg tgatcgtcat atgcttattt
    25381 ttaagcatgg cggcgacctg tgggttacgc atcccttccg ccataatttc gaccaccagc
    25441 gcccgttgcc caaaggcttc atgggtgaga gagacaatag actcgaccat atagtcaaca
    25501 aaatgaatcc ctttaatcgg ttcccggaaa cgagccagcg ccctctcctg gtcctgtaag
    25561 ataatcgcct gaatcaaggc ttctttggag atgaaatggt gatagagcgt cccgggacta
    25621 atggcgcaag atttacagat ggctttcatc gaggcactgt gaaatccatt ttctgaaaaa
    25681 atctctctgg cagcattgag gatccgggtc cgtgtcggtt gtgcttcagt ctgcatgtta
    25741 tccatcatta aaccattata gttagcgtga ataactagtt taccgcgttg tcaggtgagc
    25801 gcctatcatt gctgttgaat agtgcgaagg cacactctat tcgtatactt ttcactctga
    25861 aattggttaa ccaatagccg tgatttggct gccagttatt tagcagagac gttgatgagt
    25921 ttatcccggt aaacaacacg cacggttgtt taccggtccg ttagtgtatt aattgagctc
    25981 ctgtacccca ccaccggaga cggtgagaat ttgtccgctt acccagctcg cagcaggcga
    26041 gcaaaggaac agcgctgcgt tagcaatatc ttgcggttgg cccagacgtc tgatcggcgt
    26101 gtgctgtaac attttttgtt caatttctgg tgtaataacg gatttcaggg catcggttaa
    26161 tattgccccc ggcgcaatgc catttacccg aatatttttt tcacccaggt caaacgccat
    26221 atttctgacc agatgactgg ctgcagcttt agatgatgca taggaagtca tgtttatatt
    26281 tttattttct gccgccatag aagtgatggt cagaataacg ccaccgccat ttttttccat
    26341 ttctggcgca acaagttgtg acagatggaa aaaagaaaac acattcagtt cataagcacg
    26401 gcgaaaatcc gccattggca tatcaaacgg tttaggtcca ccgccaccgg cgttgttaac
    26461 cagaatatca actttaccca gcttactgat agcaaagtct gccagtgcag agagttcctg
    26521 ttcggaagta atatcacaac ggcaggcaaa tgcctgacca cccagttgtt gaatttcgtc
    26581 tacaacatgg ttagctgcgt cggcgttaat atcactgacc accacagatg cgccagctgt
    26641 cgcgaatgta atggcgattt ctttaccaat acctgcaccc gcacctgtga tgatggcgca
    26701 tggcgcatgg cgcatggcgc atggcgcatg gcgcatggcg catggcgcat ggcgcatggc
    26761 gcattttccg tc
//
LOCUS       DAEEKQ010000043        25970 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.043, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000043 DAEEKQ010000000
VERSION     DAEEKQ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25970
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.043"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..664
                     /locus_tag="KLK57_001716"
     CDS             <1..664
                     /locus_tag="KLK57_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HBE0892101.1"
                     /translation="SQAITNGFQRSMFSNEAGMGSTPNAAAAAASWPPHPAAQGIVQM
                     IGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQLIQKAMRVLMGSWGAEFVTLVVIL
                     FAFSSIVANYIYAENNLFFLRLNNPKAIWCLRICTFATVIGGTLLSLPLMWQLADIIM
                     ACMAITNLTAILLLSPVVHTIASDYLRQRKLGVRPVFDPLRYPDIGRQLSPDAWDDVS
                     QE"
     gene            734..1510
                     /gene="yaaA"
                     /locus_tag="KLK57_001717"
     CDS             734..1510
                     /gene="yaaA"
                     /locus_tag="KLK57_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HBE0892102.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQHLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEASSSNGELVFKRYEQR"
     gene            complement(1663..1959)
                     /locus_tag="KLK57_001718"
     CDS             complement(1663..1959)
                     /locus_tag="KLK57_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HBE0892103.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(2173..3459)
                     /gene="thrC"
                     /locus_tag="KLK57_001719"
     CDS             complement(2173..3459)
                     /gene="thrC"
                     /locus_tag="KLK57_001719"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HBE0892104.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALCDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(3460..4392)
                     /gene="thrB"
                     /locus_tag="KLK57_001720"
     CDS             complement(3460..4392)
                     /gene="thrB"
                     /locus_tag="KLK57_001720"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HBE0892105.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     SLNNLGRFADKLPSEPRENIVYQCWERFCLELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(4394..6856)
                     /gene="thrA"
                     /locus_tag="KLK57_001721"
     CDS             complement(4394..6856)
                     /gene="thrA"
                     /locus_tag="KLK57_001721"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HBE0892106.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEGELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     MERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(6883..7002)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(6938..7003)
                     /gene="thrL"
                     /locus_tag="KLK57_001722"
     CDS             complement(6938..7003)
                     /gene="thrL"
                     /locus_tag="KLK57_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HBE0892107.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(7217..7903)
                     /locus_tag="KLK57_001723"
     CDS             complement(7217..7903)
                     /locus_tag="KLK57_001723"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBE0892108.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDVIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(8303..8443)
                     /locus_tag="KLK57_001724"
     CDS             complement(8303..8443)
                     /locus_tag="KLK57_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892109.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            8539..9255
                     /gene="arcA"
                     /locus_tag="KLK57_001725"
     CDS             8539..9255
                     /gene="arcA"
                     /locus_tag="KLK57_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HBE0892110.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(9315..10667)
                     /gene="creD"
                     /locus_tag="KLK57_001726"
     CDS             complement(9315..10667)
                     /gene="creD"
                     /locus_tag="KLK57_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HBE0892111.1"
                     /translation="MLKSSLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITFGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFL
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSVDSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(10725..12149)
                     /gene="creC"
                     /locus_tag="KLK57_001727"
     CDS             complement(10725..12149)
                     /gene="creC"
                     /locus_tag="KLK57_001727"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HBE0892112.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(12149..12838)
                     /gene="creB"
                     /locus_tag="KLK57_001728"
     CDS             complement(12149..12838)
                     /gene="creB"
                     /locus_tag="KLK57_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HBE0892113.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(12851..13324)
                     /gene="creA"
                     /locus_tag="KLK57_001729"
     CDS             complement(12851..13324)
                     /gene="creA"
                     /locus_tag="KLK57_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HBE0892114.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            13535..14404
                     /gene="robA"
                     /locus_tag="KLK57_001730"
     CDS             13535..14404
                     /gene="robA"
                     /locus_tag="KLK57_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HBE0892115.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(14401..15048)
                     /gene="gpmB"
                     /locus_tag="KLK57_001731"
     CDS             complement(14401..15048)
                     /gene="gpmB"
                     /locus_tag="KLK57_001731"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HBE0892116.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            15100..15621
                     /gene="yjjX"
                     /locus_tag="KLK57_001732"
     CDS             15100..15621
                     /gene="yjjX"
                     /locus_tag="KLK57_001732"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HBE0892117.1"
                     /translation="MHQVICATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAGKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(15706..16032)
                     /gene="trpR"
                     /locus_tag="KLK57_001733"
     CDS             complement(15706..16032)
                     /gene="trpR"
                     /locus_tag="KLK57_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HBE0892118.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(16122..18059)
                     /gene="sltY"
                     /locus_tag="KLK57_001734"
     CDS             complement(16122..18059)
                     /gene="sltY"
                     /locus_tag="KLK57_001734"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HBE0892119.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQVQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDVIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQVDLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            18270..19937
                     /gene="ettA"
                     /locus_tag="KLK57_001735"
     CDS             18270..19937
                     /gene="ettA"
                     /locus_tag="KLK57_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HBE0892120.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(20244..21476)
                     /gene="nadR"
                     /locus_tag="KLK57_001736"
     CDS             complement(20244..21476)
                     /gene="nadR"
                     /locus_tag="KLK57_001736"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HBE0892121.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(21497..22879)
                     /gene="radA"
                     /locus_tag="KLK57_001737"
     CDS             complement(21497..22879)
                     /gene="radA"
                     /locus_tag="KLK57_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HBE0892122.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(22928..23896)
                     /gene="serB"
                     /locus_tag="KLK57_001738"
     CDS             complement(22928..23896)
                     /gene="serB"
                     /locus_tag="KLK57_001738"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HBE0892123.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            24002..24646
                     /locus_tag="KLK57_001739"
     CDS             24002..24646
                     /locus_tag="KLK57_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HBE0892124.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            24674..25690
                     /gene="lplA"
                     /locus_tag="KLK57_001740"
     CDS             24674..25690
                     /gene="lplA"
                     /locus_tag="KLK57_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HBE0892125.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTVEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGCLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            25722..25871
                     /locus_tag="KLK57_001741"
     CDS             25722..25871
                     /locus_tag="KLK57_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892126.1"
                     /translation="MIDLENQEREIINLMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
BASE COUNT         6214 a         7165 c         6468 g         6123 t
ORIGIN      
        1 aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg cgggaatggg
       61 ttcgacgcca aacgcggcag cggcagcggc gtcctggcct ccgcatccgg cagcgcaagg
      121 gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg caagcgccat
      181 gctgatatta ctggcgggta acgacacaac ctacatgccg ctggaaggta ttcagcttat
      241 ccagaaggcg atgcgggtgc taatgggttc ctggggtgct gagtttgtta ccctcgtggt
      301 tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa acaatctctt
      361 ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca ccttcgcaac
      421 ggtcatcggc ggcaccttgc taagtcttcc gctgatgtgg caactggcag atatcataat
      481 ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg tggttcatac
      541 cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt ttgatccgtt
      601 gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg tttcgcagga
      661 gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca taaatttcct
      721 gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt accaaagccc
      781 gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc acttgatcca
      841 tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca gcgacaaact
      901 ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc cggagaatgc
      961 ccgccaggct attttggcgt ttaaaggtga tgtctacacc ggcttgcagg ccgaaacttt
     1021 cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg gcttgtatgg
     1081 cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga tccgtcttga
     1141 gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca acaagctgaa
     1201 cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag atgagtattt
     1261 taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt tcctcgatga
     1321 gaagaacggc aaatttaaga taatcagctt ctacgctaag aaagcacgcg gtctgatgag
     1381 tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt ttaatagcga
     1441 aggttacttc tttgatgaag cttcctccag caatggcgaa ctggtgttta aacgctacga
     1501 gcagcgttaa gtttgcagtc aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa
     1561 agcatgcaga ttcaatatat tgcagaattc acgtaggcct gataagacgc gccagcgtcg
     1621 catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt gatgtttgcc
     1681 tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg gcggtggcgg
     1741 cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc accagccgtg
     1801 gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc ctatctgtaa
     1861 ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag cgaccagaac
     1921 cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct tttatcattg
     1981 cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt attctccgca
     2041 atcctaatct ctaagttacc gcatttattg agaatttctc ctttttccct gtcgtttttc
     2101 tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc tgattgagat
     2161 aatgaataga ttttactgat gattcatcat caatttacgc aacgcagcaa aatcggcggg
     2221 cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg gcagatccaa
     2281 cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg cggtgccgag
     2341 gaacaagcca tattcgcctg gattcaactg gtcacacagc gcacgatacg ctacggcagc
     2401 gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt gcgtggtttc
     2461 atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc ggaacaactc
     2521 ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata acgtcgcctg
     2581 agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat cgttcacgtt
     2641 ggtcgcagca ataaaacgtt tcaccggcag accgagtgac ttcgccagca gacccgccgt
     2701 caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg cttcctgcgg
     2761 cagctgcgca actgcttcaa agtagtagca aatctgcgcc agcaaacggc tgatgttaat
     2821 cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa acgcctgctt
     2881 caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga tattgccgcc
     2941 caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat agaggataac
     3001 cactttcaca ttcggcaaac cgtagaaagc atgagccact gccgctccgg tatcaccgga
     3061 ggtcgcggtc agaatggtca ctggcttatc gcccgcaata tgggtcagca tttgtgccat
     3121 aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt ccagacaacc
     3181 gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc gcacgcgctc
     3241 ttccaggatt tcctgcggga tttcatcacc aataaacgcc gagaggatct tcgcactgcg
     3301 ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt ccggcaggtc
     3361 atgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt gcgcaaagct
     3421 gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca gtactcgtgc
     3481 gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca ggtagttctt
     3541 acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag cgaacaaggt
     3601 cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg cctgccgcgc
     3661 ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat ctttcatcag
     3721 cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag ccagatgtcg
     3781 cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc gggcttctgc
     3841 cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa accctggcac
     3901 ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa gaaaacacgg
     3961 tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc ccatcaaagc
     4021 cagcaaacgg gtgtcattaa gtggcttgcc gcagtgttca ttcatcgcca tcagcgccgc
     4081 gaccaccgaa caggcgctgg agcctaagcc cgagccgatc ggcatattct tttccagagt
     4141 catcgccact ggaatttgct tgcccagctc caggcaaaaa cgctcccagc actgataaac
     4201 gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgaggt tgttgagact
     4261 gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat caacgggtgt
     4321 caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag ccggggcata
     4381 aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga tcggcaaaga
     4441 caccagcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac ggcagcggct
     4501 gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg aacagcggat
     4561 cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca atattgccaa
     4621 catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca aagagatcgt
     4681 cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta aactctgcgg
     4741 gcagcacagg ttcaatttca atatccgcca gttccagttc acgtcccgtt tcacgagcga
     4801 gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg tccggttcgg
     4861 tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct tcgtctaact
     4921 tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc aattcatcac
     4981 ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca acgttggtgt
     5041 catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga tagtaatcca
     5101 tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg cgcaggaagt
     5161 cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc gggttcagca
     5221 gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct ttggcttgtg
     5281 caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc agagccttcg
     5341 agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag ctttgctgac
     5401 gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca aacacttcga
     5461 taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg gtcgcatcat
     5521 cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg gcgacaatgt
     5581 tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc aaggtgcgca
     5641 taccatcacc taccaccgag ataatggcca gccgttccat caccgccagc ggctccagta
     5701 agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagcc cgcacacagt
     5761 cgctttgcgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta atcagcacca
     5821 cggaaatacg ggcgcgtgac atcgcggcaa agacgcgcgc cgccatgccg accattcctt
     5881 tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa atgcccttga
     5941 ccggtaattc gccttcatca cgactggcac caatgagcgt acctggtgct tgaggatttc
     6001 cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg gtgcgggggt
     6061 gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac atcgacttca
     6121 acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca acgtccgtcc
     6181 aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag tagtcggaac
     6241 cgttgcgtcc aagtaccacc agttcgcctt tttcattacc ggcggtgaag cctgccatca
     6301 gcaccatgtg atcagccgga atacgacttg ccgcaatacg gcgggtggac tcagcaatat
     6361 cgacggtaga ttcgaggtaa tgccccactg ccagtagttt ttcgaccgga tcgataacgg
     6421 taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac attttctcgc
     6481 cacggcaaat cagcgcagcg ttaatgctat ccgggcactg ccctaacaaa ctaatgccat
     6541 gcaggacatg ttttatttgg gcaaattcct ggtcgacgaa agttttcaat tgcgccagcg
     6601 ggaatcccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata cgttcggcat
     6661 cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc gccaccaggt
     6721 ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc ctggcattgc
     6781 tttccagaat atcggcaacc cgcagaaaac gttctgcatt tgccactgat gtaccgccga
     6841 acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa agcccgcact
     6901 gtcaggtgcg ggcttttttc tgtgtttcct gtacgcgtca gcccgcaccg ttacctgtgg
     6961 taatggtgat ggtggtggta atggtggtgc taatgcgttt catggatgtt gtgtactctg
     7021 taatttttat ctgtctgtgc gctatgccta tattggttaa agtatttagt gacctaagtc
     7081 aataaaattt taatttactc atggcaggta accagttcag aagctgctat cagacactct
     7141 ttttttaatc cacacagaga catattgccc gttgcagtca gaatgaaaag ctgaaaaata
     7201 cttactaagg cgttttttat ttggtgatat ttttttcaat atcatgcagc aaacggtgca
     7261 acattgccgt gtctcgttgc tctaaaagcc ccaggcgttg ctgtaaccag tcgaccagtt
     7321 ttatgtcatc tgccactgcc agagtcgtca gcaatgccat gactcgttcg cgtaaagctt
     7381 gcagttgatg ttggtctgcc gttgtgtcac ttttcgccgg ttgttgtatt aatgttgcta
     7441 attgatagca atagaccatc accgcctgcc ccagattgag cgaaggataa tccgccacca
     7501 tcggcacacc agtaagaacg tcagccaacg ctaactcttc gttagtcagc ccggaatctt
     7561 cgcgaccaaa caccagcgcg gcatggctca tccatgaaga tttttcctct aacagcggca
     7621 ccagttctac tggcgtggcg tagtaatgat atttcgcccg actgcgcgca gtggtggcga
     7681 cagtgaaatc gacatcgtgt aacgattcag ccagtgttgg gaaaacttta atattatcaa
     7741 taacatcacc agatccatgt gcgacccagc gggtggctgg ctccaggtgt gcctgactat
     7801 cgacaatccg cagttcgcta aaacccatcg ttttcattgc ccgcgccgct gccccaatat
     7861 tttctgctct ggcgggtgcg accaggataa tcgttatacg catattgcca ctcttcttga
     7921 ccaaataacc gcgaacaggg tgatcactgt caacttatta cgcggtgcga atttacaaat
     7981 tcttaacgta agtcgcagaa aaagcccttt acttagctta aaaaaggcta aactatttcc
     8041 tgactgtact aacggtttaa ttgttaaaaa atgctacata tccttctgtt tacttaggat
     8101 aattttataa aaaataaatc tcgacaattg gattcaccac gtttattagt tgtatgatgc
     8161 aactagttgg attattaaaa taatgtgacg aaagctagca tttagatacg atgatttcat
     8221 caaactgtta acgtgctaca attgaacttg atatatgtca acgaagcgta gttttattgg
     8281 gtgtccggcc cctcttagcc tgttatgttg ctgttaaaat ggttaggatg acagccgttt
     8341 ttgacactgt cgggtcctga gggaaagtac ccacgaccaa gctaatgatg ttgttgacgt
     8401 tgatggaaag tgcatcaaga acgcaattac gtactttagt catgttacgc cgatcatgtt
     8461 aatttgcagc atgcatcagg caggtcaggg acttttgtac ttcctgtttc gatttagttg
     8521 gcaatttagg tagcaaacat gcagaccccg cacattctta tcgttgaaga cgagttggta
     8581 acacgcaaca cgttgaaaag tattttcgaa gcggaaggct atgatgtttt cgaagcgaca
     8641 gatggcgcgg aaatgcatca gatcctctct gaatatgaca tcaacctggt gatcatggat
     8701 atcaatctgc cgggtaagaa cggtcttctg ttagcgcgtg aactgcgcga gcaggcgaat
     8761 gttgcgttga tgttcctgac tggccgtgac aacgaagtcg ataaaattct cggcctcgaa
     8821 atcggtgcag atgactacat caccaaaccg ttcaacccgc gtgaactgac gattcgtgca
     8881 cgcaacctgc tgtcccgtac catgaatctg ggtactgtca gcgaagaacg tcgtagcgtt
     8941 gaaagctaca agttcaatgg ttgggaactg gacatcaaca gccgttcgtt gatcggccct
     9001 gatggcgagc agtacaagct gccgcgcagc gagttccgcg ccatgcttca cttctgtgaa
     9061 aacccaggca aaattcagtc ccgtgctgaa ctgctgaaga aaatgaccgg ccgtgagctg
     9121 aaaccacacg accgtactgt agacgtgacg atccgccgta ttcgtaaaca tttcgaatct
     9181 acgccggata cgccggaaat catcgccacc atccacggtg aaggttatcg cttctgtggt
     9241 gatctggaag attaatcggc tttaccaccg tcaaaaaaaa cggcgctttt tagcgccgtt
     9301 tttatttttc aaccttattt ccagatacgt aattcatcgt ccgttgtaac ttctttactg
     9361 gctttcattt tcggcagtga aaacgcatac cagtcgatat tacgagtcac aaacatcatg
     9421 ccggccagcg ccaccaccag cacactggtt cccaacaaca gcgcgctatc gacagagttg
     9481 agcagtcccc acatcactcc atccagcaac aacagcgcga gggtaaacaa catgctattg
     9541 cgccagcctt tcaataccgc ctgcaaataa ataccgttca ttaacgcccc aatcagactg
     9601 gcgattatcc atgccacggt aaaaccgata tgttcagaaa gcgccagcaa gagcaaataa
     9661 aacatcacca atgaaagccc caccagcaaa tattgcattg ggtgtaaacg ttgcgcggtg
     9721 agcgtttcaa aaacaaagaa cgccataaaa gtcagtgcaa tcagcagaat ggcgtactta
     9781 gtcgcccggt ccgttaattg gtattgatcg gctggcgtcg tcactgcgac gctaaacgcg
     9841 aggaagtttt cccagccggt atcattgcct gaagcaaaac gctcaccgag attattagca
     9901 aaccagctgc tttgccattg cgcctgaaaa cctgactcgc taacttcccg tttggctggc
     9961 aaaaaatcac ctaaaaaact gggatgcggc cagttgctgg ttaaggtcat ttcgctatta
    10021 cgcccggcag gcaccacaga aagatcgccg gtaccgctta aattcagggc catattcagc
    10081 ttcaggttct gcttccgcca gtccccttcg ggtaaaggga tatgcacgcc ctgcccgcct
    10141 tgctctaacc cggtgccggg ttcaatggtc agcgccgttc cgttaacttc aggcgctttc
    10201 accacaccaa taccacgcgc atccccgacg ctaatcacaa taaatggctt gccaaaggtg
    10261 atatttggcg cgtcgagttc gctaagacgc gaaacatcga aatcggcttt tagcgttaaa
    10321 tcactgtgcc agacctgacc ggtataaatc cctatcttgc gttcttccac gttctgattg
    10381 ccatcaacca tcaatgactc aggtaaccaa aaatggataa aacttcgttt ccgctccacg
    10441 gttttatcct cttcctgtac cgtataaagc tcggtcacag gaatagcgat aagcggccca
    10501 acaagttttt gcggcccgct ggtactttgg cgaatcgcat cttccacatc gctacggtaa
    10561 tcggcacgtt cgacaatcac ctgccgaatc agcattatcg gaatcaacaa cagcaatact
    10621 gcaccaaaca gggtggtcat tttccagaac agggaggatt tcaacatagt cttctccttt
    10681 gcaatggcag caggatacga aggctatgtg ggaagaattt gaagctatgt gaagtgacgg
    10741 tgaagtcgaa gcgaggccag cacgccacct tcctgcacgt tgcgcagcgt gacttcgccg
    10801 ttaaacaaac gggcgacctc actgacgaac gccaacccca gaccgctgct tttttgccca
    10861 tttgcacgcg gcaaagagta aaagcgttca aaaatacgtg acagcgcgta gtcaggaata
    10921 ccactaccgg tatccagcac cttaagcgtg acgtgttcct gatccacttc ggcgcttagc
    10981 gttatacgac cgctctcggg ggtaaaatcg atggcgttat ccagtaaatt ccccagcgcc
    11041 tgatccagta acgccggttc agcagcaaca ttaacctcgg tgggcgtaac atgtagagtg
    11101 atgttttttt ctgccaactg cacggtgcgc gcttcgctga cgcggcggaa taatgccgcc
    11161 acatcaacag cagtcaaaac gacttcctga cgattctcca gtcttgcctg gcgtagtaac
    11221 gtttccacca gtgcctgcat tcgcgcattt tgcgtcagaa tgttgtcagt aaaacgagcc
    11281 actacttcag gcggcggacc ttcgcgtaaa atttccgccg cgccacgaat cgccgccagt
    11341 gggcttttta gctcatgggt taacgcataa acatactgct caatatagtt tttcccttcc
    11401 agcttcacgc gcatactttc cagcgcctgc gcgagtttac gcagctcgct actaccgaga
    11461 tcgggaagag gaacgggctt attgtcagtg acggaatcag catagcgagt gagccgggca
    11521 atggagcggt tgatccacca aaccatgcct gcgccaatca ccagtgcgat ccccaacaaa
    11581 atggcgctgg cccataaaat tcgccgctcg ctgcgtttaa tgaccggagc catcgccgcg
    11641 ttcggtttgc ctacgctcaa aacgccaata agccgcgagc cgtccataat cggcgcggca
    11701 acatacatca ccgaactttc gggatcggca ggattttgca acgtgctacg cgcaccatac
    11761 tgaccacgca acgttagcca gacgtcattc cagcgcgaat aatcctgccc aacggcttta
    11821 tttgccgaat cgaacaatac tttgccctgc gcatcggtca tatagacatg atattcattg
    11881 cgcactttgt taatgccacc gatattggcg cgaaacgggc gatgttgtag ctgattaaac
    11941 gcctgagcca gttgcccatg cgttgggtcc ccagagagca aatcgggacg cgccagctcc
    12001 gccagcaacg ttgcggtatc gattaacgtc ccctccgttg ctcttcgcac gcccggttta
    12061 acttctttga caaaaatagc cagtacgaac caggccgcca ccgccaccag taaaaaatag
    12121 cccagcagca accgcatgcc gatacgcatt acaggcccct caggctatat cccatgccgc
    12181 gatgagtatt aatcggagaa agatcggggt tgatggcgcg cagcttggca cgcaacgttt
    12241 taatgtgggt atcgacggtg cgatcgtagg tgtcctgcgc atcttcccac acgctatcca
    12301 ttagttgctg gcgggaccag acgcggcctg gcgacttgag taacgtcttc agcaataaaa
    12361 actcataccg cgtcagcgtt aatggcgtgt caaaccagct gatctgcgcc gcgggttcat
    12421 tcaattcaaa atgaccaata cggatgacgg gagacggcga cgagaacttc ttcacccgac
    12481 gcagtaaggt gcgcaccctg gcgcacactt cgcggggtga aaacggttta gccacatagt
    12541 cgtcagcgcc aatttccagc ccaagcaggc gatcgacctc ttcacttcgg gccgtcagga
    12601 acagtacagg taacgccgga tggagcgcca gtaactggcg gcacaattca aagccgctga
    12661 tatcaggcaa accaacatcg agaatcatga cgtcgggaac ctgctggcga gctttatcca
    12721 gcaccggcaa gcctcgctca aagacctcga cggcaaaacc ttcctgctgc aacatgtaga
    12781 ccagcgtgtc ggctatccct tgctcatctt ccactaacca gaccgtttcc cgttgcataa
    12841 atcgcctctg ttattgccgc cacggcatga caggaaccgc gctaatcgcg tttttcggcg
    12901 aaccttctac aactttgtcg gagtaagcca gataagcgag cgcgttgcgt ttggcgtcat
    12961 aaaagcgcac gacctgtaac gacttaaaga ccagggacgt gcgttttttg aatactacct
    13021 cgccctgagc tttgccgttt ttaatacgat ccgacagttc aatcggcccg acttgctgac
    13081 aagaaatagc cgcatcggag gtatcttccg ccagacccaa tcccccttta ataccacctg
    13141 ttttcgcccg gctcacataa caggtgacat ttttcacatc gggatcgtca aaggcttcca
    13201 caacaatttt gtgatccggg ccgatcattt taaatacggt gtcgaccgaa ccaatctctt
    13261 ctgcatgagc caatggcccc agcattatca ggcttaaaga caggatcaaa tgcttgtatt
    13321 tcatattgtt accattttta tctattacac gagcaattag ttcgtcacgg taaataacat
    13381 attgattaag aacaaaatct caatactttt atttccgtca agccctaaaa catactctac
    13441 taagaaaaaa acactgaatg ctaaaacagc aaaaaatgct attatccaat tacctgatgt
    13501 caggtgctcg ttgttgaaag gatgaggata ttttatggat caggccggca ttattcgcga
    13561 ccttttaatc tggctggaag gtcatctgga tcagcccctg tcgctcgaca atgtagcggc
    13621 gaaagcaggt tattccaagt ggcacttaca gagaatgttt aaagatgtca ctggccatgc
    13681 tattggcgcg tatattcgtg ctcgtcgttt gtcgaaatcg gcggtcgcac tacgcctgac
    13741 tgcgcgtccg attctggaca tcgcgctgca ataccgcttc gactctcaac agacatttac
    13801 ccgcgcattc aagaagcagt ttgcccagac tcctgcactt taccgccgtt ctcctgaatg
    13861 gagcgccttt ggtattcgcc cgccgctacg tctgggtgaa ttcacaatgc cagagcacaa
    13921 atttgtcacc ctggaagata cgccgctgat tggtgttacc cagagctact cctgttcgct
    13981 ggagcaaatc tctgatttcc gccatgaaat gcgttatcag ttctggcacg attttctcgg
    14041 caacgcgccg accattccgc cagtgctcta cggcctgaat gaaacgcgtc cgagtcagga
    14101 taaagacgac gaacaagagg tattctatac caccgcgtta gcccaggatc aggcagatgg
    14161 ctatgtactg acggggcatc cggtgatgct gcagggcggc gaatatgtga tgtttaccta
    14221 tgaaggtctg ggaaccggcg tgcaggagtt tatcctgacg gtatacggaa cgtgcatgcc
    14281 aatgctcaac ctgacgcgcc gtaaaggtca ggatattgag cgatactacc cggcagaaga
    14341 cgccaaagcg ggagatcgcc caattaatct gcgctgtgaa ctgctgattc ctatccgccg
    14401 ttaacgctgc agctcatcta atgcaggggc gtccagatgc gagatgtccc ctgcggtttc
    14461 aacgacccag ccagacgcca gccacaggct ttcctgataa tccacgcgcg aaatagaaca
    14521 gttacgcaga cgtaagcggc gctctgccca tgctggtaat ccgagaatcg tactcaccag
    14581 acatcccagt gcaataccat ggctcaccag cagcggtcgg cttccctgcg gtaagtcccg
    14641 gcaagattcc agtgccgcat tgacgcgatc gctgagctcc tgcattgact cgccttcagg
    14701 aatacgcccg tcaacggtgc cattgaccag ctgccgacgc cagttctctt cttcttcggt
    14761 cagagaatcg atatgtcttt tctccagcac acccatgttt aattcacgca ggcgagaatc
    14821 aaagatgatg tcacagccgc aggcctgggc gatgatttcc gccgtacgcc gggtgcgtcc
    14881 taaatcgcta ctgataatat gcgtaatgcc aagctctttg gcacgggttg ccacctgcat
    14941 cgcctgttgc tcacctttgg cggtcagcgg gctgtcagac tggccctgaa tacgtcgctc
    15001 ggcgttccac tgcgtttcac cgtggcggac taggtatacc tgtaacatgc tttttttccg
    15061 tatactgctg taacgttaat tttctgagta atgctgatta tgcaccaagt tatctgtgcg
    15121 accaccaatc ccgctaaaat tcaggccatt ctgcaggcat ttcacgagat cttcggcgaa
    15181 ggatcctgcc atattgcatc cgttgccgtc gagagcggtg taccggaaca gccctttggc
    15241 agtgaggaaa cgcgcgctgg cgcacgaaat cgggtagcaa atgcccgccg tttacttcca
    15301 gaggctgatt tttgggtggc gattgaagct ggcatcgatg gtgacagcac tttcagttgg
    15361 gtggtgattg aaaacaccag ccagcgcggc gaggcgcgtt cggctacttt gccgttacct
    15421 gcggtgattc tgcagaaagt gcgtgaaggt gaggcgcttg gtcccgtgat gtcgcgttat
    15481 accggtattg atgagattgg tcgtaaggaa ggggcgatcg gcgtgtttac cgccgggaaa
    15541 ctcactcgcg ccagcgtcta tcaccaggcg gtgatcctgg cgctgagtcc gtttcacaat
    15601 gccgtgtatc aggcattgta ggacggataa ggcgttcacg ccgcatccgg cattcggtgc
    15661 acgatgcctg atgcgacgct gccgcgtctt atcaggccta caaaatcaat cgcttttcag
    15721 caacacctct tccagccact ggcgcagctc cacgggcgcg gctttcaggc tgttagatcc
    15781 acgcgtaatc gtcgcgatgc ccgcgccgag ttcatttttt aactcacgct gactcatttc
    15841 gccacgcaac agctcttcga caatacgcac gcgagtcccc aacgcttcgc gctcatctgg
    15901 cgtcagcatc aggtttaaca acggtaaatg gagatcgttt tggtaggcat tcttaagcag
    15961 gtcgacaaaa cgtaaccact cctggtgacg ctgttctgcc atcgctgctg aatagggtga
    16021 ttgttgggcc ataatatgtc gccattgtta gcgggggaag caaaatgcct cccccggttg
    16081 tactcgctaa agagtacgat agcatatcat aaacgtgcgg atcagtaacg acgtccccat
    16141 tccgtggcgc tcatcaacgt cggtttatcc cccatgaaat aacggtagta agcgtcataa
    16201 gccagcacgt tcttcacata accgcgcgtc tcggagaacg gaatactctc gacaaatgcc
    16261 acagcgtcga tacgcccggc gctgttgcca agccaggttc gcacccgtcc aggtccggcg
    16321 ttataagctg ctgaggagaa aatacggtta ttgccaaact gctgataaac atattgcagg
    16381 taactggtgc caatgttgat attcgtttcc ggatccagca attgcccagg gctgctataa
    16441 ccaggaatag agaacatctt caccgtatgg gtcgctgtac caggcataat ctgcatcagg
    16501 ccgctggccc ctaccggtga tttcactttc ggattccagg cgctctcctg acgggcaatc
    16561 gccatcgcat agctttgcgg gatctctttc ccactggtgt aacgtttgaa aagatcgttg
    16621 taagccagcg ggaatcgctc ttccagatga tcccaaagct tcccggcgat cgttgcctga
    16681 acgctaagat cccaccattg gttattgaaa gcataccgag ccagttgagc ctgctctgtt
    16741 tttgacttgc tcttcaccag attggcccac tcgctacgcg cggtgttatc gagattccag
    16801 tacatcaact cgcgcacgcg cgccatctcc ggcccctgag tcagggcgct gtcaacattc
    16861 tgcggcgctt tatcaatctt cagctcatac tcttctccga tgcgttgtgc tgcaaccatc
    16921 gggtagaaac cacgctgttg catgagttga tgcaaaatct ctttcgcttc agcttcacgt
    16981 ccgcgttcca gcaataaatc cacctgccag taacgccatt catctttctc ttttgcttcc
    17041 atcggcagac gcgccagcca ggtattcagg ccgcgacgat cgccggtgcc aagtgccatt
    17101 cgcacacggc gttcaatcaa cgacgtggat tgcgagcgca taatgacgtc atcgcgccat
    17161 ttcgcctgct cgtcggtgac atcgttgccc atcaaacgcc aggcgacgat atcgcgcagc
    17221 tcctgaatct gatcttcatt aagctgctgc acctgggcaa gcgacgggat catcagccgt
    17281 gcattttcag catcctgccg cgccacactg gcaaacgcca ccgctgccat ttgacgggta
    17341 aaatcggtcg ctccggttgt acgcgcgaag gtcagtaccg tattagggtt gttcgccagt
    17401 gaaatgattg ccgaggcgat agtctggtaa tcggcaggca tctgccctgc cagcactgtt
    17461 accaggccag tgttacccgc tttcatcgcc agacggatac gctctaaata cgccagcgga
    17521 tcttgtttac ctgacgcacg ccagacgcta aataacttgt cacaggcgtt aggctggctc
    17581 ttgccggtta gccacagctc tttcgcccct tgccaggctt cttcactctg cccggtgttc
    17641 catttcgcat agtagtaatt acattgcgct tcggtagttc cgggcttttc cgggctaaag
    17701 gctaacaagc cacgccagtc ttcacgccgc gccagttcat tgacgaaacg agattgcagc
    17761 gtgcgagcgg gaggaagcgt ggggttagcg cgaacaaagt tagtgaccgt caccgccggt
    17821 tgattcatca gatcatcggt gatctggcgg tattccaggt agggataaag cggataatcc
    17881 ttcagtccag gcatcatttg ttcgaccaca tccatttgtc gattatccca ggcctgcttg
    17941 atttgcgcgt aacggctacg ctgctcatcc agtgagtcgg ctcgcgccac gctgctgacc
    18001 gtcagcagac agacaccggc agccaacagc cgccaggtaa cttgtttggc tttttccaca
    18061 agcgcatcct ctaagtgtaa atacatcaat gcagcgtcat gccgcgtaat agatttatgc
    18121 taaccagtca ttgccgttta cgccacgtta cggacacttt tttactttta ctgcgaaggt
    18181 gatcgaccaa ctgggattag gcggggaaaa aggctacact tcgcctttga aaactcatca
    18241 tcacgataaa catagaggcg aagtccaacg tggctcaatt cgtttatacc atgcatcgtg
    18301 tcggcaaagt tgttccgccg aaacgtcata ttttgaaaaa catctctctg agtttcttcc
    18361 ctggggcaaa aattggtgtc ctgggtctga acggcgcggg taagtctacc ctgctgcgca
    18421 ttatggcggg cattgataaa gacatcgaag gtgaagcgcg tccgcagcca gacatcaaga
    18481 ttggttacct gccgcaggaa ccgcagctga acccggaaca caccgtgcgt gagtccattg
    18541 aagaagcggt ttctgaagtg gttaacgccc tgaaacgcct ggatgaagtg tatgcgctgt
    18601 acgccgatcc ggatgccgat tttgacaagc tggccgctga acaaggccgt ctggaagaga
    18661 tcattcaggc tcacgacggt cataacctga acgtacagct ggagcgtgcg gcggatgcgc
    18721 tacgtctgcc ggactgggac gcgaaaatcg ctaacctctc cggtggtgag cgtcgtcgcg
    18781 tagcgttgtg ccgcctgctg ctggaaaaac cagacatgct gctgctcgac gaaccgacca
    18841 accacctgga tgccgaatcc gtggcctggc tggaacgctt cctgcacgac ttcgagggca
    18901 ccgtggtggc gattacccac gaccgttact tcctcgataa cgttgcaggc tggatcctcg
    18961 aacttgaccg cggtgaaggt attccgtggg aaggcaacta ctcctcctgg ctggagcaga
    19021 aagatcagcg cctggcgcag gaagcttcac aagaagcggc gcgtcgtaag tcgatcgaga
    19081 aagagctgga gtgggtacgt cagggaacta aaggccgcca gtcgaaaggt aaagcacgtc
    19141 tggcacgctt tgaagagctg aacagcaccg aatatcagaa acgtaacgaa accaacgaac
    19201 tgtttattcc acctggaccg cgtctgggcg ataaagtgct ggaagtcagc aacctgcgta
    19261 aatcctacgg tgatcgcctg ctgattgatg acctgagctt ctcgatcccg aaaggggcaa
    19321 tcgtcgggat catcggtccg aacggcgcgg gtaaatcgac cctgttccgt atgatctctg
    19381 gtcaggaaca gccggacagc ggcaccatca ctttaggtga aacggtgaaa ctggcgtcgg
    19441 ttgatcagtt ccgtgactca atggataaca gcaaaaccgt ttgggaagaa gtttccggcg
    19501 ggctggatat tatgaagatc ggcaacaccg agatgccaag ccgcgcctac gttggccgct
    19561 ttaactttaa aggggttgat cagggtaaac gcgttggtga actttccggt ggtgagcgcg
    19621 gtcgtctgca tctggcgaag ctgctgcagg ttggcggcaa catgctgctg ctcgacgaac
    19681 caaccaacga cctggatatc gaaaccctgc gcgcgctgga aaacgccctg ctggagttcc
    19741 cgggctgtgc gatggttatc tcgcacgacc gttggttcct cgaccgtatc gccacgcaca
    19801 tcctggacta ccaggatgaa ggtaaagttg agttcttcga aggtaacttt actgagtacg
    19861 aagagtacaa gaaacgcacg ctgggcgcag acgcactgga gccgaagcgt atcaagtaca
    19921 agcgtattgc gaagtaatgc gtaaaatgcc ggatgcggcg tgaacgcctt atccggccta
    19981 caaaccaaga aatttcaata cgttgtgaat ctccaggcct gataagacgc ggtaagcgtc
    20041 gcatcaggca ttgagcaccg aatgccggat acggcgtgaa cgccttttcc ggcctacaac
    20101 agccttagta tccgcaattt cgtagcgggt cacccgtgca ggttttttca aaccttgttt
    20161 aatctcgaca gcctcttcca gagaggtttt caactcatca aaaaacttat tttatacctc
    20221 gcctttgagc cgtttcatcg cggttatctc tgctccccca tcatctcccg caccagctcc
    20281 acgcagcgca ggaaacggct gtcataatcg tcctcttcaa cccgcacgaa ttcgatattg
    20341 ttctcttcca gcatctccac cagcaagttc tggaactctt tgcgatccac cgaactgccg
    20401 aggctgcgta aaccatccgc cacccacggc gtgttgttct ccagcaggat caccagatcg
    20461 aaacggtatt catcaatcaa cgcctgtacg aacggatgct cacgcccttc gtactttttg
    20521 cagaacgcct gggtagtgac aaaatcagtg tcaataaacg ccactttatt ggcatatttc
    20581 actgcaaaat caatgtattg tgcgtggccc agcgcgattt tatcgtaatc ggaatactgc
    20641 aatgcgatct catcaccgcc gaggtgtgaa aagacataat cgcgaccata ttcccacgca
    20701 ctggtggtat tgaagatatt ggcaagtttg tttaccaggg tggatttacc gctcgactcg
    20761 ccaccaagga tcgccacggt acgtacaaag aacggcttca cttccgtagg aatatattcc
    20821 cagtagcgga acgggttttc gcggatctgc gcaccgctga tactcataaa ggtacgtttc
    20881 ggatcgacca gcaccgtatc gatccccaga tgttccatat actgtggcgc atcggcttct
    20941 tccgaggtgt agatcaggtc cggctgaatc cccttttcag ccataaactt tttgatgccg
    21001 ttgctccaca catcccagcc gtgcggatac ggctccatgc cctcttcgtt gaaagcatga
    21061 atgcgaatat ttttctgata tttaaaagtt tgtaataacc agcgcagacg atccggcacc
    21121 gtgggctgct gcgacatggc gctgtcttca aacaacgcgc ggtcgcgggt atcgtcaaaa
    21181 cccataatga tatgcagctc gtcaacctgg ctacaggcgc gctggataag gtagatatgt
    21241 ccggtatgca gcgggtagaa cttaccgaat acgacaccaa tggttttctt ctgtcgggga
    21301 aattcaagcc ccaaaaaacg gtgcaacgcc tccagctttt gcgcgctggg gcttttaatt
    21361 ttggcattca gtaactggct taaataccct ttggtcatac cgctggcatc agctacctgc
    21421 tgtagcgtgc agccctgttg cttgatggca gttttcaggt aatcaaatga cgacatatct
    21481 ccctccgtat ctctcattat aagtcgtcga acacgctaag cgcgtcggag agttttttaa
    21541 cgccaaaaat ctgcatccct tccggcgctt ttttcggtac gttagccgcc ggaacaatcg
    21601 cccggcgaaa accgtgtttc gccgcttctg aaattcgttc ctgaccgctg ggcaccgggc
    21661 ggatctcccc tgccagcccg acttcaccaa acaccaccag atcctgtggc agcggtctgt
    21721 cacgcaggct ggaaaccatc gccagcagta acgctaaatc ggcactggtt tcggttacct
    21781 tcacgccgcc gaccacgttc acaaatacat cctgatcggc catttgcaga ccaccgtgac
    21841 ggtgcaagac agccagcagg attgccagac ggttttgttc cagccccact gccacgcggc
    21901 gcggattcgc catcatcgag tgatcgacca gcgcctgaat ctccaccagc aacggacgcg
    21961 ttccttccca caccaccatc acggagctgc cggaggtcac ttcatctccg cgacttaaga
    22021 aaattgccga agggttgctg acttcacgca gcccctgttc ggtcatcgcg aagacgccca
    22081 gctcattcac cgcgccgaag cggtttttat ggctgcgcaa ggtgcgaaaa cgggagtcgg
    22141 catcaccatc cagaagcacc gaacagtcga tacagtgttc cagcacttta gggccagcca
    22201 gcgagccatc tttggttacg tgcccaacca tgacaatcgc cacaccgcgc gttttggcga
    22261 agcgcgtcag ataagccgcc gtttcacgca cctgcgccac gctaccaggc gatgattgta
    22321 catccgccat atgcatcacc tggatcgagt caattaccat cagtttcggt tgctcttctt
    22381 cggcaatcag gcagatctgt tcgatgctgg tttccgacaa catattgaga ttgtcagtcg
    22441 gcaggccaag gcgatgagcg cgcattgcca cctgttgcag cgactcttcg ccagtgacat
    22501 acagcgtttt catctgctgg gccagtttgc acagcgtttg cagtagcagc gtggatttcc
    22561 ccgcacctgg gttaccgcca atcagaatgg cgcttcccgg caccacgccg ccgcctagta
    22621 cgcggtcgaa ctctttaaat ccggtggaaa aacgcggcag ctcttcaagg ctgatatcgg
    22681 agagtttctg gacttttgcc accccggcgc taccggcata gccgctgaga cgctcgttac
    22741 gcgccaccgt tggcgacgca gcaagacgca cctcggtgat ggtgttccag gcatgacagg
    22801 cactgcactg cccctgccag cgcggataat cggccccgca ttcattacaa acaaaggcgc
    22861 gttttggagc ttttgccacg gtgtacctcg ttaatgctgt gccgcccgca ggatggcggg
    22921 cgagcaatta cttctgattc aggctgccag agaggatgca gaataccccc atcaggtcag
    22981 cgtgacggat ggtgacttcc gccttttcat tcacttttgg cttggcatgg taggcaatcc
    23041 ccagccctgc tgctttgatc atcggcaggt cattagctcc atcgccaatc gccacggtct
    23101 gcgccagcgg gatttcatac tcctgcgcga ggcgagtcaa agttttcgct ttgtactgcg
    23161 cgtctacgat gtcgccaatc acattgccgg taaatttacc gtccatgatc tccagttcat
    23221 tggctaccac ggcggtcagg cgcagcttgt cgcgcagata ttcagcaaag aaagtaaagc
    23281 cgccggaggc aatcgccact ttccagccca gcgtttccag cttgagcacc agttgcgtta
    23341 agcctggcat cagcggcaga ttttcacgca cctgttgcag aatattggcg tcagcgcctt
    23401 tcagcgtcgc cacacggctg cgcaggctgg cggtaaaatc gagttcgccg cgcatcgccc
    23461 gttcggttac ttccgccacc atctcgcccg ttccggccag tttggcaatt tcatcaatac
    23521 attcaatctg gatggcggtg gaatccatgt ccatcaccag caaacccggc gtgcgcaggt
    23581 gcgggatttt ccccagcggg gcgacatcca gttgcgcttc atgggccagg cgcgtagccc
    23641 gtgcggtgag tgaacctgcc agacgaatca cctgataatc ttccacgcac caggcggcaa
    23701 caatcaccat cgccgcaccc agtttgctct ggtattgggt cagacgctgt ttatccagcc
    23761 cacgaccata cagcagccag ccgctacgac ctgcgtggta atccagtggc atcacttcat
    23821 caccacttaa tgaaagaggc agacccggcc ataaagagac atcttcaggc aggtcgcacc
    23881 aggtaatgtt aggcattaag gctcctgtaa aatcgttcga agcagggaaa ataacgcatg
    23941 aggctacctt gtatccattg cttctggcaa cattaagtct caaattttca aagggtggaa
    24001 gatggctcgc acaaaactga aattccggct gcatcgggca gtgattgtcc tgttctgtct
    24061 tgccttgtta gtggcactga tgcagggggc gtcatggttt agtcaaaacc accagcgaca
    24121 gcgtaatcca cagctggaag aactggcccg caccctggcg cgtcaggtga cgctgaacgt
    24181 tgcaccgctg atgcgtaccg actcaccaga tgaaaaacgc attcaggcga tcctcgatca
    24241 gttaacggat gaaagccgca tcctcgacgc gggtgtgtat gacgaacaag gcgatcttat
    24301 cgcccgttca ggcgaaagcg tcgaagtgcg cgaccggctg gcgctcgacg gtaaaaaagc
    24361 aggcggctat tttaaccagc agattgtcga gccaattgcg ggtaaaaacg gaccgctcgg
    24421 ctatctgcgc ctgacactcg acacccatac gctcgccacc gaagcccaac aggtggataa
    24481 caccactaac attttacgcc tgatgttgct gctctcactg gcaatcggcg tagtgctgac
    24541 ccgcacgctg ctacagggta aacgcacccg ctggcagcaa tcgcccttcc tgttaaccgc
    24601 cagcaaaccg gtgccggaag aggaagaaag cgagaaaaaa gagtgaccca ttactacaag
    24661 aaaggaaatc gttatgtcca cattacgcct gctcatctct gactcttacg acccgtggtt
    24721 taacctggcg gtggaagagt gtatttttcg ccaaatgccc gccacgcagc gcgttctgtt
    24781 tctctggcgc aatgccgaca cggtagtgat tggtcgcgcg cagaacccgt ggaaagagtg
    24841 taatacccgg cggatggaag aagataacgt ccgcctggcg cggcgcagta gcggtggcgg
    24901 cgcggtgttc cacgatctcg gcaatacctg ctttaccttt atggctggca agccggagta
    24961 cgataaaacc atctctacgt cgattgtgct caatgcgctg aacgcgctcg gcgtcagcgc
    25021 cgaagcgtcc ggacgtaacg atctggtggt gaaaacagtc gaaggcgacc gcaaagtctc
    25081 aggctcggcc tatcgcgaaa ccaaagatcg tggcttccac cacggcactt tgctgctcaa
    25141 tgccgacctt agccgcctgg caaactatct caatccggat aaaaagaaac tggcggcgaa
    25201 aggcattacc tcagtgcgtt cccgcgtgac caacctcacc gagctgctgc cggggatcac
    25261 ccatgagcag gtttgcgagg ccataaccaa ggcctttttc gcccattatg gcgagcgtgt
    25321 agaagcggaa atcatctccc cggacaaaac gccagacttg ccaaacttcg ccgaaacctt
    25381 tgcccgtcag agtagctggg aatggaactt cggtcaggct ccggcattct cgcatctgct
    25441 ggatgaacgc tttagctggg gcggcgtgga actgcatttc gacgttgaaa aaggccatat
    25501 cacccgcgcc caggtgttta ccgacagcct caaccccgcg ccgctggaag ccctcgccgg
    25561 gcgactgcaa ggctgcctgt accgcgcgga tatgctgcaa caagagtgcg aagcgctgtt
    25621 ggttgacttc ccggaccagg aaaaagagct acgggagttg tcgacgtgga tagcgggggc
    25681 ggtaaggtaa ctcgctgatt aaaataccaa aggaagggaa tatgatcgat ctggaaaacc
    25741 aggagcggga gattatcaat ttaatgttaa gccagcgtat ttcctggcta gccgccgtgc
    25801 gtatcaggca taaattatca cttgctgaag taagtaaaat gttaggtatc tcgattaatt
    25861 ctctaaaata gatcgaaaag acggaacggc ttagctcaaa catcaaaagc aaaatgggag
    25921 aaatttatgg ttgtccacca gagttgctga tatgcccttc ctggatgacg
//
LOCUS       DAEEKQ010000044        25823 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.044, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000044 DAEEKQ010000000
VERSION     DAEEKQ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25823
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.044"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            16..486
                     /locus_tag="KLK57_001742"
     CDS             16..486
                     /locus_tag="KLK57_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HBE0892127.1"
                     /translation="MVDQNLPWSASLKVGDKKLPVVVVRVGEASVDVLVGQQTWTLTH
                     KWFESVWTGDYLLLWKMSPEGESTITRDSSEEEILWLETMLNRALHISTEPSAEWRPL
                     LVEKIKQFQKSHHLKTDGVVGFSTLVHLWQVAGESAYLYRDEANISPETTVKGK"
     gene            488..907
                     /gene="gspB"
                     /locus_tag="KLK57_001743"
     CDS             488..907
                     /gene="gspB"
                     /locus_tag="KLK57_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative general secretion pathway protein GspB"
                     /protein_id="HBE0892128.1"
                     /translation="MFEFYIAAREQKETGHPGIFSRQKHSTIIYVICLLLICLWFAGM
                     VLVGGYARQLWALWIVKAEVTVEAETPAFKQSTQHYFFKKQPLPVVESVEEEDDPDVA
                     VENAPSSSEDEENTVKESDEKAGLRERVKNALNELER"
     gene            1145..1456
                     /gene="rpsJ"
                     /locus_tag="KLK57_001744"
     CDS             1145..1456
                     /gene="rpsJ"
                     /locus_tag="KLK57_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HBE0892129.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            1489..2118
                     /gene="rplC"
                     /locus_tag="KLK57_001745"
     CDS             1489..2118
                     /gene="rplC"
                     /locus_tag="KLK57_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HBE0892130.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     EVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2129..2734
                     /gene="rplD"
                     /locus_tag="KLK57_001746"
     CDS             2129..2734
                     /gene="rplD"
                     /locus_tag="KLK57_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HBE0892131.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            2731..3033
                     /gene="rplW"
                     /locus_tag="KLK57_001747"
     CDS             2731..3033
                     /gene="rplW"
                     /locus_tag="KLK57_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HBE0892132.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3051..3872
                     /gene="rplB"
                     /locus_tag="KLK57_001748"
     CDS             3051..3872
                     /gene="rplB"
                     /locus_tag="KLK57_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HBE0892133.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            3889..4167
                     /gene="rpsS"
                     /locus_tag="KLK57_001749"
     CDS             3889..4167
                     /gene="rpsS"
                     /locus_tag="KLK57_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HBE0892134.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4182..4514
                     /gene="rplV"
                     /locus_tag="KLK57_001750"
     CDS             4182..4514
                     /gene="rplV"
                     /locus_tag="KLK57_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HBE0892135.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            4532..5233
                     /gene="rpsC"
                     /locus_tag="KLK57_001751"
     CDS             4532..5233
                     /gene="rpsC"
                     /locus_tag="KLK57_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HBE0892136.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5246..5656
                     /gene="rplP"
                     /locus_tag="KLK57_001752"
     CDS             5246..5656
                     /gene="rplP"
                     /locus_tag="KLK57_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HBE0892137.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            5656..5847
                     /gene="rpmC"
                     /locus_tag="KLK57_001753"
     CDS             5656..5847
                     /gene="rpmC"
                     /locus_tag="KLK57_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HBE0892138.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            5847..6101
                     /gene="rpsQ"
                     /locus_tag="KLK57_001754"
     CDS             5847..6101
                     /gene="rpsQ"
                     /locus_tag="KLK57_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HBE0892139.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6266..6637
                     /gene="rplN"
                     /locus_tag="KLK57_001755"
     CDS             6266..6637
                     /gene="rplN"
                     /locus_tag="KLK57_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HBE0892140.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            6648..6962
                     /gene="rplX"
                     /locus_tag="KLK57_001756"
     CDS             6648..6962
                     /gene="rplX"
                     /locus_tag="KLK57_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HBE0892141.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            6977..7516
                     /gene="rplE"
                     /locus_tag="KLK57_001757"
     CDS             6977..7516
                     /gene="rplE"
                     /locus_tag="KLK57_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HBE0892142.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            7531..7836
                     /gene="rpsN"
                     /locus_tag="KLK57_001758"
     CDS             7531..7836
                     /gene="rpsN"
                     /locus_tag="KLK57_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HBE0892143.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            7870..8262
                     /gene="rpsH"
                     /locus_tag="KLK57_001759"
     CDS             7870..8262
                     /gene="rpsH"
                     /locus_tag="KLK57_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HBE0892144.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8275..8808
                     /gene="rplF"
                     /locus_tag="KLK57_001760"
     CDS             8275..8808
                     /gene="rplF"
                     /locus_tag="KLK57_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HBE0892145.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGVDKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            8818..9171
                     /gene="rplR"
                     /locus_tag="KLK57_001761"
     CDS             8818..9171
                     /gene="rplR"
                     /locus_tag="KLK57_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HBE0892146.1"
                     /translation="MDKKFARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9186..9689
                     /gene="rpsE"
                     /locus_tag="KLK57_001762"
     CDS             9186..9689
                     /gene="rpsE"
                     /locus_tag="KLK57_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HBE0892147.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            9693..9872
                     /gene="rpmD"
                     /locus_tag="KLK57_001763"
     CDS             9693..9872
                     /gene="rpmD"
                     /locus_tag="KLK57_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HBE0892148.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            9876..10310
                     /gene="rplO"
                     /locus_tag="KLK57_001764"
     CDS             9876..10310
                     /gene="rplO"
                     /locus_tag="KLK57_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HBE0892149.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10318..11649
                     /gene="secY"
                     /locus_tag="KLK57_001765"
     CDS             10318..11649
                     /gene="secY"
                     /locus_tag="KLK57_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HBE0892150.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFVGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            11681..11797
                     /gene="rpmJ"
                     /locus_tag="KLK57_001766"
     CDS             11681..11797
                     /gene="rpmJ"
                     /locus_tag="KLK57_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HBE0892151.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            11944..12300
                     /gene="rpsM"
                     /locus_tag="KLK57_001767"
     CDS             11944..12300
                     /gene="rpsM"
                     /locus_tag="KLK57_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HBE0892152.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12317..12706
                     /gene="rpsK"
                     /locus_tag="KLK57_001768"
     CDS             12317..12706
                     /gene="rpsK"
                     /locus_tag="KLK57_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HBE0892153.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            12740..13360
                     /gene="rpsD"
                     /locus_tag="KLK57_001769"
     CDS             12740..13360
                     /gene="rpsD"
                     /locus_tag="KLK57_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HBE0892154.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            13386..14375
                     /gene="rpoA"
                     /locus_tag="KLK57_001770"
     CDS             13386..14375
                     /gene="rpoA"
                     /locus_tag="KLK57_001770"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HBE0892155.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            14416..14799
                     /gene="rplQ"
                     /locus_tag="KLK57_001771"
     CDS             14416..14799
                     /gene="rplQ"
                     /locus_tag="KLK57_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HBE0892156.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            14906..15274
                     /locus_tag="KLK57_001772"
     CDS             14906..15274
                     /locus_tag="KLK57_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HBE0892157.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLDKINDN"
     gene            15285..15710
                     /gene="zntR"
                     /locus_tag="KLK57_001773"
     CDS             15285..15710
                     /gene="zntR"
                     /locus_tag="KLK57_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HBE0892158.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            15766..15984
                     /gene="arfA"
                     /locus_tag="KLK57_001774"
     CDS             15766..15984
                     /gene="arfA"
                     /locus_tag="KLK57_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HBE0892159.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGAC"
     gene            complement(15981..16391)
                     /gene="mscL"
                     /locus_tag="KLK57_001775"
     CDS             complement(15981..16391)
                     /gene="mscL"
                     /locus_tag="KLK57_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HBE0892160.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLAEIRDLLKEQNNRS"
     gene            complement(16521..17897)
                     /gene="trkA"
                     /locus_tag="KLK57_001776"
     CDS             complement(16521..17897)
                     /gene="trkA"
                     /locus_tag="KLK57_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HBE0892161.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(17919..19208)
                     /gene="rsmB"
                     /locus_tag="KLK57_001777"
     CDS             complement(17919..19208)
                     /gene="rsmB"
                     /locus_tag="KLK57_001777"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HBE0892162.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNTSDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVHDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(19254..20201)
                     /gene="fmt"
                     /locus_tag="KLK57_001778"
     CDS             complement(19254..20201)
                     /gene="fmt"
                     /locus_tag="KLK57_001778"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HBE0892163.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQESQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLA"
     gene            complement(20216..20725)
                     /gene="def"
                     /locus_tag="KLK57_001779"
     CDS             complement(20216..20725)
                     /gene="def"
                     /locus_tag="KLK57_001779"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HBE0892164.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            20855..21979
                     /gene="dprA"
                     /locus_tag="KLK57_001780"
     CDS             20855..21979
                     /gene="dprA"
                     /locus_tag="KLK57_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBE0892165.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLLVTTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQRREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            21951..22424
                     /locus_tag="KLK57_001781"
     CDS             21951..22424
                     /locus_tag="KLK57_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HBE0892166.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            22453..22995
                     /locus_tag="KLK57_001782"
     CDS             22453..22995
                     /locus_tag="KLK57_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HBE0892167.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RLLKSSADGHIVKVLEGQVCPACGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23000..23572
                     /gene="tsaC"
                     /locus_tag="KLK57_001783"
     CDS             23000..23572
                     /gene="tsaC"
                     /locus_tag="KLK57_001783"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HBE0892168.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            23577..24395
                     /gene="aroE"
                     /locus_tag="KLK57_001784"
     CDS             23577..24395
                     /gene="aroE"
                     /locus_tag="KLK57_001784"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HBE0892169.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKLLQQELSA"
     gene            24392..24649
                     /locus_tag="KLK57_001785"
     CDS             24392..24649
                     /locus_tag="KLK57_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HBE0892170.1"
                     /translation="MNQAIQFPDREEWDENKRCVCFPALVNGMQLTCAISGESLAYRF
                     TGGTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(24625..25179)
                     /locus_tag="KLK57_001786"
     CDS             complement(24625..25179)
                     /locus_tag="KLK57_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HBE0892171.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            25652..>25823
                     /locus_tag="KLK57_001787"
     rRNA            25652..>25823
                     /locus_tag="KLK57_001787"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6591 a         6087 c         6765 g         6380 t
ORIGIN      
        1 gcttctttac aaacattggt agatcagaat ttgccctgga gcgcatcgct taaagtcggg
       61 gacaaaaaat tgcctgttgt ggtcgttcgc gtcggtgagg ctagcgttga tgtcctcgta
      121 ggtcagcaaa cgtggacgct gacacataaa tggtttgagt cggtatggac gggtgattat
      181 cttctgttgt ggaaaatgtc gccggaaggg gagagtacca ttacgcgtga tagcagcgag
      241 gaagagattc tctggctgga aacgatgtta aaccgtgcat tgcatatttc gacagaacct
      301 tcggcggagt ggcgtccctt gttggtagaa aaaataaagc aatttcaaaa aagccaccac
      361 ttgaaaactg acggtgttgt tggcttttca acgttagtac atctctggca ggtggcaggg
      421 gagagtgcct acttatatcg ggatgaagca aatatttccc cggaaacaac agtgaagggg
      481 aaatgacatg tttgaattct atatcgccgc gcgtgagcag aaagaaacag gtcaccctgg
      541 tatcttctcc cgacaaaaac acagcaccat aatatatgtc atttgtttat tgctgatttg
      601 cctgtggttt gcaggaatgg tgttggttgg tgggtatgcc aggcagctat gggcgctttg
      661 gatagtaaaa gcagaagtca ctgtagaggc tgaaacgccc gcttttaaac aatcaacaca
      721 acactatttc ttcaaaaaac agccactccc cgtcgtcgaa tccgtcgagg aggaagatga
      781 cccagacgtc gcggttgaga atgctccctc ttcttctgaa gacgaagaaa atactgtaaa
      841 agaaagcgac gagaaagccg gtttaagaga gcgtgtcaaa aatgcgctga acgaacttga
      901 gagataaccc gaaggctgtt tactttacta gcaatacgct tgcgttcggt ggttaagtat
      961 gtataatgcg cgggcttgtc gtagttgaca gcaggttcaa tctgaacctc agttagccga
     1021 atttggctac ctaacaatgc tcccaatcgg ggagctacgt aagaacggtt acactctccc
     1081 atcaatcgta atgggtctga ggagtaatca ttttcgttta taaaataatt ggagctctgg
     1141 tctcatgcag aaccaaagaa tccgtatccg cctgaaagcg tttgatcatc gtctgatcga
     1201 tcaagcaacc gcggaaatcg tcgagactgc caagcgcact ggtgcgcagg tccgtggtcc
     1261 gatcccgctg ccgacacgca aagagcgctt cactgttctg atctccccgc acgtcaacaa
     1321 agacgcgcgc gatcagtacg aaatccgtac tcacttgcgt ctggttgaca tcgttgagcc
     1381 aaccgagaaa accgttgatg ctctgatgcg tctggatctg gctgccggtg tagacgtgca
     1441 gatcagcctg ggttaatcag gtcattgagc gattgagagg ttgaaacaat gattggttta
     1501 gtcggtaaaa aagtgggtat gacccgtatc ttcacagaag acggcgtttc tatcccagta
     1561 accgtaatcg aagttgaagc aaaccgcgtt actcaggtta aagacctggc taacgatggc
     1621 taccgtgcta ttcaggtgac caccggtgct aaaaaagcta accgtgtgac caagcctgaa
     1681 gctggccact tcgctaaagc tggcgtagaa gctggccgtg gtctgtggga attccgcctg
     1741 gctgaaggcg aagagttcac tgtaggtcag agcattagcg ttgaactgtt tgctgaggtt
     1801 aaaaaagttg acgtaactgg cacctctaaa ggtaaaggtt tcgcaggtac cgttaagcgc
     1861 tggaacttcc gtacccagga cgctactcac ggtaactcct tgtctcaccg cgttccgggt
     1921 tctatcggtc agaaccagac tccgggcaaa gtgttcaaag gcaagaaaat ggcaggtcag
     1981 atgggtaacg aacgtgtaac cgttcagagc cttgacgtag tacgcgttga cgctgagcgc
     2041 aacctgctgc tggttaaagg tgctgtcccg ggtgcaaccg gtagcgacct gatcgttaaa
     2101 ccagctgtga aggcgtaagg agatagcaat ggaattagta ttgaaagacg cgcagagcgc
     2161 gctgactgtt tccgaaacta ccttcggtcg tgatttcaac gaagcgctgg ttcaccaggt
     2221 tgttgttgct tatgcagctg gtgctcgtca gggtactcgt gctcagaaga ctcgtgctga
     2281 agtaactggt tccggtaaaa aaccgtggcg ccagaaaggc accggccgtg cgcgttctgg
     2341 ttctatcaag agcccgatct ggcgttctgg tggcgtgacc tttgctgctc gtccgcagga
     2401 ccacagtcaa aaagttaaca agaagatgta ccgcggcgcg ctgaaaagca tcctgtccga
     2461 actggtacgt caggatcgtc tgatcgttgt cgagaagttc tctgtagaag cgccgaaaac
     2521 taagctgctg gcacagaaac tgaaagacat ggctctggaa gatgtgctga tcatcaccgg
     2581 tgagctggac gaaaacctgt tcctggctgc gcgcaacctg cacaaggttg acgtacgcga
     2641 tgcaactggt atcgacccgg ttagcctgat cgccttcgac aaagtcgtaa tgactgctga
     2701 tgctgttaag caagttgagg agatgctggc atgattcgtg aagaacgtct gctgaaggtg
     2761 ctgcgtgcac cgcacgtttc tgaaaaagcg tctactgcga tggaaaaatc caacaccatc
     2821 gtactcaaag ttgctaaaga cgcgaccaaa gcagaaatca aagctgctgt gcagaaactg
     2881 tttgaagtcg aagtcgaagt cgttaacacc ctggtagtta aagggaaagt taaacgtcac
     2941 ggacagcgta tcggtcgtcg tagcgactgg aaaaaagctt acgtcaccct gaaagaaggc
     3001 cagaatctgg acttcgttgg cggcgctgag taagtcggag gagtaataca atggcagttg
     3061 ttaaatgtaa accgacatct ccgggtcgtc gccacgtagt taaagtggtt aaccctgagc
     3121 tgcacaaggg caaacctttt gctccgttgc tggaaaaaaa cagcaaatcc ggtggtcgta
     3181 acaacaatgg ccgtatcacc actcgtcata tcggtggtgg ccacaagcag gcttaccgta
     3241 ttgttgactt caaacgcaac aaagacggta tcccggcagt tgttgaacgt cttgagtacg
     3301 atccgaaccg ttccgcgaac atcgcgctgg ttctgtacaa agacggtgaa cgccgttaca
     3361 tcctggcccc taaaggcctg aaagctggcg accagattca gtctggcgtt gatgctgcaa
     3421 tcaaaccagg taacaccctg ccgatgcgca acatcccggt tggttctact gttcataacg
     3481 tagaaatgaa accaggtaaa ggcggtcagc tggcacgttc cgctggtact tacgttcaga
     3541 tcgttgctcg tgatggtgct tatgtcaccc tgcgtctgcg ttctggtgaa atgcgtaaag
     3601 tagaagcaga ctgccgtgca actctgggcg aagttggcaa tgctgagcat atgctgcgcg
     3661 ttctgggtaa agcaggtgct gcacgctggc gtggtgttcg tccgaccgtt cgcggtaccg
     3721 cgatgaaccc ggtagaccac ccacatggtg gtggtgaagg tcgtaacttt ggtaagcacc
     3781 cggtaactcc gtggggcgtt cagaccaaag gtaagaagac ccgcagcaac aagcgtactg
     3841 ataaattcat cgtacgtcgc cgtagcaaat aattttagag gataagccat gccacgttct
     3901 ctcaagaaag gtccttttat tgacctgcac ttgctgaaga aggtagagaa agcggtggaa
     3961 agcggagaca agaagcccct gcgcacttgg tcccgtcgtt caacgatctt tcctaacatg
     4021 atcggtttga ccatcgctgt ccataatggt cgtcagcacg ttccggtatt tgtgaccgac
     4081 gaaatggtcg gtcacaaact gggtgaattc gcaccgactc gtacttatcg cggccacgct
     4141 gctgataaaa aagcgaagaa gaaataaggt aggaggaaga gatggaaact atcgctaaac
     4201 atcgccatgc tcgttcttct gctcagaagg ttcgccttgt tgctgacctg attcgcggta
     4261 agaaagtgtc gcaggctctg gatattttga cctacaccaa caagaaagcg gctgtactgg
     4321 tcaagaaagt tctggaatct gccattgcta acgctgaaca caacgatggc gctgacattg
     4381 acgatctgaa agttacgaaa attttcgtag acgaaggccc gagcatgaag cgcattatgc
     4441 cgcgtgcaaa aggtcgtgca gatcgcatcc tgaagcgcac cagccacatc actgtggttg
     4501 tgtccgatcg ctgagactct ggagactagc aatgggtcag aaagtacatc ctaatggtat
     4561 tcgcttgggt attgtaaaac catggaactc tacctggttt gcgaacacca aagaattcgc
     4621 tgacaacctg gacagcgatt ttaaagtacg tcagtacctg actaaggaac tggctaaagc
     4681 gtctgtatct cgtatcgtta tcgagcgtcc ggctaagagc atccgtgtaa ccattcacac
     4741 tgctcgcccg ggtatcgtta tcggtaaaaa aggtgaagac gtagaaaaac tgcgtaaggt
     4801 cgtagcggac atcgctggcg ttcctgcaca gatcaacatc gccgaagttc gtaagcctga
     4861 actggacgca aaactggttg ctgacagcat cacttctcag ctggaacgtc gcgttatgtt
     4921 ccgtcgtgct atgaagcgtg ctgtacagaa cgcaatgcgt ctgggcgcta aaggtattaa
     4981 agttgaagtt agcggccgtc tgggcggcgc ggaaatcgca cgtaccgaat ggtaccgcga
     5041 aggtcgcgta ccgctgcaca ctctgcgtgc tgacatcgac tacaacacct ctgaagcgca
     5101 caccacttac ggtgtaatcg gcgttaaagt gtggatcttc aaaggcgaga tcctgggtgg
     5161 tatggctgct gttgaacaac cggaaaaacc ggctgctcag cctaaaaagc agcagcgtaa
     5221 aggccgtaaa taaggagcgt cgctgatgtt acaaccaaag cgtacaaaat tccgtaaaat
     5281 gcacaaaggc cgtaaccgcg gtctggcgca gggtacggat gttagcttcg gcagcttcgg
     5341 tctgaaagct gttggccgtg gtcgtctgac tgcccgtcag atcgaagcag cacgtcgtgc
     5401 tatgacccgt gcagttaagc gtcaaggtaa gatctggatc cgtgtgttcc cggacaaacc
     5461 gatcactgaa aagccgctgg cagtgcgtat gggtaaaggt aaaggtaacg tggagtattg
     5521 ggttgccttg attcagccgg gtaaagtcct gtatgaaatg gacggtgttc cggaagagct
     5581 ggcccgtgaa gcattcaagc tggcagcagc gaaactgccg attaaaacca cctttgtaac
     5641 taagacggtg atgtaatgaa agcaaaagag ctgcgtgaga agagcgttga agagctgaac
     5701 accgagctgc tgaacctgct gcgtgagcag ttcaacctgc gtatgcaggc tgcaagtggc
     5761 cagctgcaac agtctcacct gttgaagcaa gtgcgtcgcg atgtcgcacg cgttaagact
     5821 ttactgaacg agaaggcggg tgcgtaatga ccgataaaat ccgtactctg caaggtcgcg
     5881 ttgttagcga caaaatggag aaatccattg ttgttgctat cgaacgtttt gtgaaacacc
     5941 cgatctacgg taaattcatc aagcgtacga ccaaactgca cgtacatgac gagaacaacg
     6001 aatgcggtat cggtgacgtg gttgaaatcc gcgaatgccg tccgctgtcc aagactaaat
     6061 cctggacgct ggttcgcgtt gtagagaaag cggttctgta atacagtaca ctctctcgat
     6121 acgaataaac ggctcagaaa tgagccgttt attttttcta cccatatcct tgaagcggtg
     6181 ttataatgcc gcgccctcga tatggggatt tttaacgacc tgattttcgg gtctcagtag
     6241 tagttgacat tagcggagca ctaaaatgat ccaagaacag actatgctga acgtcgccga
     6301 caactccggt gcacgtcgcg taatgtgtat caaggttctg ggtggctcgc accgtcgcta
     6361 cgcaggcgta ggcgacatca tcaagatcac catcaaagaa gcaattccgc gtggtaaggt
     6421 caaaaaaggt gatgtgctga aggcggtagt ggtgcgcacc aagaagggtg ttcgtcgccc
     6481 ggacggttct gtcattcgct tcgatggtaa tgcttgtgtt cttctgaaca acaacagcga
     6541 gcagcctatc ggtacgcgta tttttgggcc ggtaactcgt gagcttcgta gtgagaagtt
     6601 catgaaaatt atctctctgg caccagaagt actctaagga gcgaatcatg gcagcgaaaa
     6661 tccgtcgtga tgacgaagtt atcgtgttaa ccggtaaaga taaaggtaaa cgcggtaaag
     6721 ttaagaatgt cctgtcttcc ggcaaggtca ttgttgaagg tatcaacctg gttaagaaac
     6781 atcagaagcc ggttccggcc ctgaaccaac cgggtggcat cgttgaaaaa gaagccgcta
     6841 ttcaggtttc caacgtagca atcttcaatg cggcaaccgg caaggctgac cgtgtaggct
     6901 ttagattcga agacggcaaa aaagtccgtt tcttcaagtc taacagcgaa actatcaagt
     6961 aatttggagt agtacgatgg cgaaactgca tgattactac aaagacgaag tagttaaaaa
     7021 actcatgact gagtttaact acaattctgt catgcaagtc cctcgggtcg agaagatcac
     7081 cctgaacatg ggtgttggtg aagcgatcgc tgacaaaaaa ctgctggata acgcagcagc
     7141 agacctggca gcaatctccg gtcaaaaacc gctgatcacc aaagcacgca aatctgttgc
     7201 aggcttcaaa atccgtcagg gctatccgat cggctgtaaa gtaactctgc gtggcgaacg
     7261 catgtgggag ttctttgagc gcctgatcac tattgctgta cctcgtattc gtgacttccg
     7321 tggcctgtcc gctaagtctt tcgacggtcg tggtaactac agcatgggtg tccgtgagca
     7381 gatcatcttc ccagaaatcg actacgataa agtcgaccgc gttcgtggtt tggatattac
     7441 cattaccact actgcgaaat ctgacgaaga aggccgcgct ctgctggctg cctttgactt
     7501 cccgttccgc aagtaaggta gggttactaa atggctaagc aatcaatgaa agcacgcgaa
     7561 gtaaaacgcg tagctttagc tgataaatac ttcgcgaaac gcgctgaact gaaagcgatc
     7621 atctctgatg tgaacgcttc cgacgaagat cgttggaacg ctgttctcaa gctgcagact
     7681 ctgccgcgtg attccagccc gtctcgtcag cgtaaccgct gccgtcaaac aggtcgcccg
     7741 catggtttcc tgcggaagtt cgggttgagc cgtattaagg tccgtgaagc cgctatgcgc
     7801 ggtgaaatcc cgggtctgaa aaaggctagc tggtaattgt caccaattga atcacgggag
     7861 taaagacaga tgagcatgca agatccgatc gcggatatgc tgacccgtat ccgtaacggt
     7921 caggccgcga acaaagctgc ggtcaccatg ccttcctcca agctgaaagt ggcaatcgcc
     7981 aacgtgctga aggaagaagg ttttattgaa gattttaaag ttgaaggcga caccaagcct
     8041 gaactggaac ttactctgaa gtatttccag ggcaaagctg ttgtagaaag cattcagcgt
     8101 gtcagccgcc caggtctgcg catctataaa cgtaaagatg agctgccgaa agttatggcg
     8161 ggtctgggta tcgcagttgt ttctacctct aaaggtgtta tgactgatcg tgcagcgcgc
     8221 caggctggtc ttggtggcga aattatctgc tacgtagcct aatcggagga aaaaatgtct
     8281 cgtgttgcta aagcaccggt cgttgttcct gccggcgttg acgtaaaaat caacggtcag
     8341 gttattacga tcaaaggtaa aaacggcgag ctgactcgta ctctcaacga tgctgttgaa
     8401 gttaaacatg cagataatac cctgaccttc ggtccgcgtg atggttacgc agacggttgg
     8461 gcacaggctg gtaccgcgcg tgccctgctg aactcaatgg ttatcggtgt taccgaaggc
     8521 ttcactaaga agctgcagct ggttggtgta ggttaccgtg cagcggttaa aggcaatgtg
     8581 attaacctgt ctctgggttt ctctcatcct gttgaccatc agctgcctgc gggtatcact
     8641 gctgaatgtc cgactcagac tgaaatcgtg ctgaaaggcg ttgataagca ggtgatcggc
     8701 caggttgcag cggatctgcg cgcctaccgt cgtcctgagc cttataaagg caagggtgtt
     8761 cgttacgccg acgaagtcgt gcgtaccaaa gaggctaaga agaagtaagg taacactatg
     8821 gataagaaat ttgctcgtat ccgtcgtgcg acccgcgcac gccgcaagct ccaggagctg
     8881 ggcgcaactc gcctggtggt acatcgtacc ccgcgtcaca tttacgcaca ggtaattgca
     8941 ccgaacggtt ctgaagttct ggtagctgct tctactgtag aaaaagctat cgctgaacaa
     9001 ctgaagtaca ccggtaacaa agacgcggct gcagctgtgg gtaaagctgt cgctgaacgc
     9061 gctctggaaa aaggcatcaa agatgtatcc tttgaccgtt ccgggttcca atatcatggt
     9121 cgtgtccagg cactggcaga tgctgcccgt gaagctggcc ttcagttcta aggtagaggt
     9181 gtaagatggc tcacatcgaa aaacaagctg gcgaactgca ggaaaagctg atcgcggtaa
     9241 accgcgtatc taaaaccgtt aaaggtggtc gtattttctc cttcacagct ctgactgtag
     9301 ttggcgatgg taacggtcgc gttggttttg gttacggtaa agcgcgtgaa gttccagcag
     9361 cgatccagaa agcgatggaa aaagcccgtc gcaatatgat taacgtcgcg ctgaataacg
     9421 gcactctgca acaccctgtt aaaggtgttc acacgggttc tcgcgtattc atgcagccgg
     9481 cttccgaagg taccggtatc atcgccggtg gtgcaatgcg cgccgttctg gaagtcgctg
     9541 gggttcataa cgttctggct aaagcatatg gttccaccaa cccgatcaac gtggttcgtg
     9601 caactattga tggcctggaa aatatgaatt ctccagaaat ggtcgctgcc aagcgtggta
     9661 aatccgttga agaaattctg gggaaataaa ccatggcaaa gactattaaa attactcaaa
     9721 cccgcagtgc aatcggtcgt ctgccgaaac acaaggcaac gctgcttggc ctgggtctgc
     9781 gtcgtattgg tcacaccgta gagcgcgagg atactcctgc tattcgcggt atgatcaacg
     9841 cggtttcctt catggttaaa gttgaggagt aagagatgcg tttaaatact ctgtctccgg
     9901 ccgaaggctc caaaaaggcg ggtaaacgct tgggtcgtgg tatcggttct ggcctcggta
     9961 aaaccggtgg tcgtggtcac aaaggtcaga agtctcgttc tggcggtggc gtacgtcgcg
    10021 gtttcgaggg tggtcagatg cctctgtacc gtcgtctgcc gaaattcggc ttcacttctc
    10081 gtaaagcagc gattacagcc gaagttcgtc tgtctgacct ggctaaagta gaaggcggtg
    10141 ttgtagacct gaacacgctg aaagcggcta acattatcgg tatccagatc gagttcgcga
    10201 aagtgatcct ggctggcgaa gtaacgactc cggtaactgt tcgtggcctg cgtgttacta
    10261 aaggcgctcg tgctgctatc gaagctgctg gcggtaaaat cgaggaataa gtagcagatg
    10321 gctaaacaac cgggattaga ttttcaaagt gccaaaggtg gcttaggcga gctgaaacgc
    10381 agactgctgt ttgttatcgg tgcgctgatt gtgttccgta ttggctcttt tattccgatc
    10441 cctggtattg atgccgctgt acttgccaaa ctgcttgagc aacagcgagg caccatcatt
    10501 gagatgttta acatgttctc tggtggtgct ctcagccgtg cttctatctt tgctctgggg
    10561 atcatgccgt atatttcggc gtcgatcatt atccagctgc tgacggtggt tcacccaacg
    10621 ttggcggaaa ttaagaaaga aggggagtct ggtcgtcgta agatcagcca gtacacccgc
    10681 tacggtactc tggtgctggc aatattccag tcgatcggta ttgctactgg tctgccgaat
    10741 atgcctggta tgcaaggcct ggtgattaac ccgggctttg cattctactt caccgctgtt
    10801 gtaagtctgg tcacaggaac gatgttcctg atgtggttgg gcgaacagat tactgaacga
    10861 ggtatcggca acggtatttc aatcattatc ttcgtcggta ttgtcgcggg actcccgcca
    10921 gccattgccc atactatcga gcaagcgcgt caaggcgacc tgcacttcct cgtgttgctg
    10981 ttggttgcag tattagtatt tgcagtgacg ttctttgttg tatttgttga gcgtggtcaa
    11041 cgccgcattg tggtaaacta cgcgaaacgt cagcaaggtc gtcgtgttta tgctgcacag
    11101 agcacacatt taccgctgaa agtgaatatg gcgggggtaa tcccggcaat cttcgcttcc
    11161 agtattattc tgttcccggc gaccatcgcg tcatggttcg ggggcggtac tggttggaac
    11221 tggctgacaa caatttcgct gtatttgcag cctgggcaac cgctttatgt gttactctat
    11281 gcgtctgcaa tcatcttctt ctgtttcttc tacacggcgt tggttttcaa cccgcgtgaa
    11341 acagcagata acctgaagaa gtccggtgca tttgtaccag gaattcgtcc gggagagcaa
    11401 acggcgaagt atatcgataa agtaatgacc cgcctgaccc tggttggtgc gctgtatatt
    11461 acctttatct gcctgatccc ggagttcatg cgtgatgcaa tgaaagtacc gttctacttc
    11521 ggtgggacct cactgcttat cgttgttgtc gtgattatgg actttatggc tcaagtgcaa
    11581 actctgatga tgtccagtca gtatgagtct gcattgaaga aggcgaacct gaaaggctac
    11641 ggccgataat tggtcgcccg agaagttacg gagagtaaaa atgaaagttc gtgcttccgt
    11701 caagaaatta tgccgtaact gcaaaatcgt taagcgtgat ggtgtcatcc gtgtgatttg
    11761 cagtgccgag ccgaagcata aacagcgcca aggctgattt tttcgcatat ttttcttgca
    11821 aagttgggtt gagctggcta gattagccag ccaatctttt gtatgtctgt gcgtttccat
    11881 ttgagtatcc tgaaaacggg cttttcagca tggaacgtac atattaaata gtaggagtgc
    11941 atagtggccc gtatagcagg cattaacatt cctgatcata agcatgccgt aatcgcatta
    12001 acttcgattt atggcgtcgg caagacccgt tctaaagcca tcctggctgc agcgggtatc
    12061 gctgaagatg ttaagatcag tgagctgtct gaaggacaaa tcgacacgct gcgtgacgaa
    12121 gttgccaaat ttgtcgttga aggtgatctg cgccgtgaaa tcagcatgag catcaagcgc
    12181 ctgatggatc ttggttgcta tcgcggtttg cgtcatcgtc gtggtctccc ggttcgcggt
    12241 cagcgtacca agaccaacgc acgtacccgt aagggtccgc gcaaaccgat caagaaataa
    12301 tcggggtgat tgaataatgg caaaggcacc aattcgtgca cgtaaacgtg taagaaaaca
    12361 agtctctgac ggcgtggctc atatccatgc ttctttcaac aacaccatcg tgactatcac
    12421 tgatcgtcag ggtaacgcgt tgggttgggc aacagccggt ggttccggtt tccgtggttc
    12481 tcgcaaatcc actccgtttg cagctcaggt tgcagcagag cgttgcgctg acgccgtgaa
    12541 agaatacggc atcaagaatc tggaagttat ggttaaaggt ccgggtccag gccgcgaatc
    12601 tactattcgt gctctgaacg ccgcaggttt ccgcatcact aacattactg atgtgactcc
    12661 gatccctcat aacggttgtc gtccgccgaa aaaacgtcgc gtataacgcc tcgttttcca
    12721 ggtttgttgg agaaagaaaa tggcaagata tttgggtcct aagctcaagc tgagccgtcg
    12781 tgagggcacc gacttattcc ttaagtctgg cgttcgcgcg atcgatacca agtgtaaaat
    12841 tgaacaagct cctggccagc acggtgcgcg taaaccgcgt ctgtctgact atggtgtgca
    12901 gttgcgtgaa aagcaaaaag ttcgccgtat ctatggtgtg ctggagcgtc agttccgtaa
    12961 ctactacaaa gaagcagcac gtctgaaagg caacaccggt gaaaacctgt tggctctgct
    13021 ggaaggtcgt ctggacaacg ttgtataccg tatgggcttc ggtgccactc gtgcagaagc
    13081 acgtcagctg gttagccata aagcaattat ggtaaacggt cgtgttgtta acatcgcttc
    13141 ttatcaggtt agtccgaatg acgttgtaag cattcgtgag aaagcgaaga agcagtctcg
    13201 cgtgaaagcc gctctggagc tggctgagca gcgtgaaaag ccaacttggc tggaagttga
    13261 tgctggcaag atggaaggta cgtttaagcg taagccggag cgttctgatc tgtctgcgga
    13321 cattaacgaa cacctgatcg tcgagcttta ctccaagtaa agcttagtac caaagagagg
    13381 acacaatgca gggttctgtg acagagtttc taaaaccgcg cctggttgat atcgagcaag
    13441 tgagttcgac gcacgccaag gtgacccttg agcctttaga gcgtggcttt ggccatactc
    13501 tgggtaacgc actgcgccgt attctgctct catcgatgcc gggttgcgcg gtgaccgagg
    13561 ttgagattga tggtgtacta catgagtaca gcaccaaaga aggcgttcag gaagatatcc
    13621 tggaaatcct gctcaacctg aaagggctgg cggtgagagt tcagggcaaa gatgaagtta
    13681 ttcttacctt gaataaatct ggcattggcc ctgtgactgc agccgatatc acccacgacg
    13741 gtgatgtcga aatcgtcaag ccgcagcacg tgatctgcca cctgaccgat gagaacgcgt
    13801 ctattagcat gcgtatcaaa gttcagcgcg gtcgtggtta tgtgccggct tctacccgaa
    13861 ttcattcgga agaagatgag cgcccaatcg gccgtctgct ggtcgacgca tgctacagcc
    13921 ctgtggagcg tattgcctac aatgttgaag cagcgcgtgt agaacagcgt accgacctgg
    13981 acaagctggt catcgaaatg gaaaccaacg gcacaatcga tcctgaagag gcgattcgtc
    14041 gtgcggcaac cattctggct gaacaactgg aagctttcgt tgacttacgt gatgtacgtc
    14101 agcctgaagt gaaagaagag aaaccagagt tcgatccgat cctgctgcgc cctgttgacg
    14161 atctggaatt gactgtccgc tctgctaact gccttaaagc agaagctatc cactatatcg
    14221 gtgatctggt acagcgtacc gaggttgagc tccttaaaac gcctaacctt ggtaaaaaat
    14281 ctcttactga gattaaagac gtgctggctt cccgtggact gtctctgggc atgcgcctgg
    14341 aaaactggcc accggcaagc atcgctgacg agtaaccgga tcacaggtta aggttttact
    14401 gagaaggata aggtcatgcg ccatcgtaag agtggtcgtc aactgaaccg caacagcagc
    14461 catcgccagg ctatgttccg caatatggca ggttcactgg ttcgtcatga aatcatcaag
    14521 acgactctgc ctaaagcgaa agagctgcgc cgcgtagttg agccgctgat tactcttgcc
    14581 aagactgata gcgttgctaa tcgtcgtctg gcatttgccc gtactcgtga taacgagatc
    14641 gtggcaaaac tgtttaacga actgggcccg cgtttcgcga gccgtgccgg tggttacact
    14701 cgtattctga agtgtggctt ccgtgcaggc gacaacgcgc cgatggctta catcgagctg
    14761 gttgatcgtt cagagaaagc agaagctgct gcagagtaat ctgaagcaat gtaaaaaaac
    14821 ccgccccggc gggttttttt atacccgtag tatccccact tatctacaat agctgtactc
    14881 tttttgttca tcccctggag tatttatgtg gttacttgac cagtgggcag agcgccatat
    14941 agcagaagcg caagcgaaag gtgagtttga taacctggca ggtagcggcg aaccattgat
    15001 actggatgat gattctcacg tgccaccgga attacgtgcg gggtatcgct tgctgaagaa
    15061 tgccggttgc ttaccgccag aacttgagca acggagagaa gcaattcagc ttctggatat
    15121 tctcaaaggt atccgtcacg atgatccgca atatcaagag gttagccgtc gattgtcatt
    15181 actggaattg aagctgcgac aagctggatt gagtaccgat tttttacgcg gcgattatgc
    15241 tgacaagttg ttggacaaaa tcaacgataa ctagtggagt atgtatgtat cgcattggtg
    15301 agctggcaaa aatggcggaa gtaacacctg acacgattcg ttattacgaa aaacagcaga
    15361 tgatggagca tgaagtgcgt actgaaggcg ggtttcgcct gtataccgaa agcgatctcc
    15421 agcgattgaa atttatccgc catgccagac aactaggttt cagtctggag tcgatccgcg
    15481 agttgctgtc gatccgcatc gatcctgaac accatacctg tcaggagtca aaaggcattg
    15541 tgcaggaaag attgcaggaa gtcgaagcac ggatagccga gttgcagagt atgcagcgtt
    15601 ccttgcaacg ccttaacgat gcctgttgtg ggaccgccca tagcagtgtt tattgctcga
    15661 ttcttgaagc tcttgaacaa ggggcgagtg gcgttaagag tggctgttga ttttttgcac
    15721 tgacaggatt acactcgcgc cgttaaataa ccaactggag tttttatgag ccgatatcag
    15781 catactaaag ggcagataaa ggataatgcg atagaagcat tactacatga tcccttattc
    15841 cgacagcgag tagagaaaaa taagaaaggg aaaggcagtt acatgcgaaa aggaaaacat
    15901 ggcaatcggg ggaactggga ggccagtggc aagaaagtaa atcacttttt taccactggt
    15961 cttctgcttt caggcgcttg ttaagagcgg ttattctgct ctttcagcaa atcacgaatt
    16021 tctgccagta atacttcttc tttagttggt gcaggtgcgg cagctggttc ttcttttttc
    16081 cgattcagtt tgttgattag cttaatcgcc ataaagatgg caaaggccac aatcagaaaa
    16141 tcaaagacgt tttgaatgaa gacaccgtaa tgcatcacaa cagcagggat atccccctgc
    16201 gcatcgcgta gcgtgacagc aaactgttta aagtcgatcc cgccaattaa taagcccaga
    16261 ggtggcatga tgatatcggc aaccagtgaa gatacaatct tcccgaatgc cgcaccgata
    16321 atgacaccca ccgccaaatc caccacgttc ccgcgcatcg caaattcgcg aaattcttta
    16381 ataatgctca tgttattctc cctatgaagc cgacaaccat aagtctaaca aatgttaagc
    16441 cattttcctg ccaggaatga atttaattaa tccttgataa tcaaagggaa ataataaggc
    16501 gtcattagac gccttattaa ttacaagaag aaagggcttg gctggaagag tctttcgacg
    16561 tcggtaataa actttttatc tgtgaggaac ataattacgt gatcgccttg ctcaatgcgc
    16621 agattgtcat tggcaatcat cacgtcgttt ccacgtacca ccgctccaat aatcgttcct
    16681 ggcggtagct tgatttcgtc aatgactctg ccgacaacgc gtgaggtgct ttcatcaccg
    16741 tgagcaacgg cttcaatagc ttctgctacg ccgcggcgca atgaggaaac accaacaata
    16801 tctgcttttc gcacatggct aagcaacgca gaaatagttg cttgttgtgg tgaaattgca
    16861 atatcgatca cgctcccctg aaccagatcc acataagcgc gacgctggat caataccatc
    16921 acctttttcg cacccatacg tttggcaagc atggcggaca tgatattggc ctcgtcatcg
    16981 ttggtgacgg caataaacag atcaacttga tcgatatgtt cttcggccag cagttcttga
    17041 tccgacgcat caccaaaaaa gacgatcgta ttctgcaact tttccgccag ttcggcagcg
    17101 cgctgctgat tacgttcgat gagtttaacg ctgtaatctt tttccagacg acgcgccagc
    17161 cctgcaccga tattaccgcc accaaccagc atgatccgct tatacggttt ttccagtcgc
    17221 tgtaattcac tcatcaccgc gcggatatgc tgtgaagcgg caataaagaa cacttcatca
    17281 ccagcttcaa caatggtcga accttgtgga cgaatggggc gatcgtggcg gaaaattgct
    17341 gccacacgag tatcgatatg tggcatatgt tcgcgcatgg tcgaaagtgc attaccaatc
    17401 agcgggccgc cataataggc tttaactaca gcgaggctga ctttaccctc agcgaagttc
    17461 accacctgca atgcgcctgg atactcaatc agtcggtaaa tattatcgat aaccaactgc
    17521 tctggtgcga tcagatgatc aatcggtaca gcatctgaat gaaatagctt atcggcatcg
    17581 cgtacgtagt ctggtgagcg gatgcgagcg atgcgattag gggtgttgaa aagtgagtag
    17641 gctacctggc aggcaaccat attggtttca tctgaactgg ttacagcaac cagcatatcg
    17701 gcgtcgtcgg cacctgcctc ccgcaatacg cgtggatgag agccatgccc ctgcacgact
    17761 cgcaggtcaa atttatcctg caaggtccgc agacgctcac cgttggtatc gacaacagta
    17821 atatcgttgt tctcgccaac caggttttcc gccagtgtgc cgccaacctg gccggcaccc
    17881 agaatgataa ttttcatcag tctcgacccg ttatctcatc actttttgat tagcttagcg
    17941 taaaagaagc cgtcgccctc ttcggcacca ggcagatttt gtttacccgg ttgctctggt
    18001 gttcctgttt cgcaaagttc ggcatcagcg gtacgttgca aaaaagcttt aatctgcagg
    18061 ctattctctt ccggtaacac cgaacaggtg gcatagacca gggttccacc ggattttaaa
    18121 tgcggccaaa tggcgtcgag aatttcagac tgcaattgcg cgagttccgg gatatcgcga
    18181 tcgcgacgta accatttaat atccggatgg cgacgaatca cccccgttgc tgaacaaggc
    18241 gcatctaata aaatgcgatc aaactgttgc tcgccacacc attgggaagg gtaacggcca
    18301 tcaccttgtt tcacggtcgc cttcatacca aggcgtttta aattgtcgtg aacgcgagag
    18361 aggcgctgtt cgtcaatatc aaccgcaaca acctgcgctt ctggtgccac ctcaaggata
    18421 tgcgttgttt taccgccggg ggcggcacaa agatccagaa tgtgttcacc gttttgtggc
    18481 gcaagccagg tcatgcaacc ttgtgctgat gcatcctgaa cggtaaccca tccgtcttca
    18541 aaaccaggta gcgcatgaac aggtgcaggt gtttccagcc gtacagcatc agggtaatcc
    18601 gcatgcggga aacctttcat tcctgcttca tccaacaatg caagccagct gtcgcgggaa
    18661 tgatgcgtac gattaacgcg cagccacatt ggcggacgct ggttattggc ttcgacgatg
    18721 gattgccact gctctggata cgctttttgc agacgcttca gcaaccagga aggatgcaga
    18781 taacgtgcat cactggtatt aaactcggct aataactctt cttgctgacg ctggaactgg
    18841 cgtaatacac cgttaatcaa ccctttaagt tgcggacgct taattgcgat agcgccttca
    18901 accgtttcag ccagcgcagc atgaggtgga atgcgggtat aaagcagttg atacaaacca
    18961 accataatca ggtaatgcac agtgcgctgt ttgccggtca tcggacgggc cattaactta
    19021 ttaatcagcc agtctaactg cgaaagcgta cgcagtacgc caaagcacaa ctcttgaaga
    19081 agtgctttgt ctttatcaga aactttttgc tggagcggtg gcagaatgtt gcttaatgat
    19141 tgcccttgct cgacgacttg ttcaacggcc tgggccgcca tgctacgtaa attacgttgt
    19201 tttttcataa gtataaaaat gcccggtaag accgggctta gaagagtgga ctatcaggcc
    19261 agacggttgc ccggaacaaa ccattcccga cgagaattca ggaggtcttg cgcgctcatc
    19321 gctttcttac ccgcaggttg taacgagagc aggttcagga tgccatcacc agtcgcaacc
    19381 tgaatgcctt gtttgttggc ttcaaggatc gttcctggtg cagcgttggt tgccgtatca
    19441 atgaccgacg ctttccagac tttaaccggt tgtccttcaa tttccagcca gctcattggc
    19501 catgggttga aagcgcgaat gcagcgttca agctgcgctg ccgaaagcga ccagtcaata
    19561 cgcgcttctt ctttactcaa cttctcggcg taagtgacaa gagtttcgtc ctgaacttct
    19621 ggtttcgccg tgccgtctgc tagttgtttc aacgtggtga ttagcccttg tgggccaagc
    19681 tctgccagct tgtcgtacag cgtaccactg gtatcttctg cagtaatcgg gcaggagagc
    19741 ttatagagca tatcaccggt gtctaaaccg acatccattt gcataatggt cacaccagtt
    19801 tctgcatcac ccgcccatag tgagcgttgg attggtgcag caccgcgcca gcgtggcagc
    19861 agtgaaccat gaacgttgat acagccaaga cgcggcatct ccagcactgc tttaggcaga
    19921 attaagccat aggcgacgac gaccataaca tcagcctgca gatcggcgac cagttgctgg
    19981 ctttcttgtg gacgcaggga aacaggttga aaaacgggca gacctttttc ctcagccaga
    20041 actttaaccg ggctgggcat cagtttttta ccgcgtcctg ccggtcggtc tggctgggtg
    20101 aacacgccaa cgacgttatg accagaagac aacagcgcgt cgagatgacg cgctgcaaag
    20161 tcaggtgtac ccgcaaaaat aatacgtagt gattctgaca cgttagttct tatccttaag
    20221 cccgggcttt cagacgatct agtttttcaa ctttctgacg aatacgttgt tgtttcagcg
    20281 gtgacagata atccataaac agtttgccga ccaggtgatc catctcatgc tgaatacaga
    20341 tggctaacag cccctctgct tccagttcaa atggtttacc gtcgcggtca agggcgcgaa
    20401 ttttaacttt ctctgcgcgc ggcactaaag cacgttgttc agggatcgac aggcaacctt
    20461 cttcaatgcc tgtttcgccg cttttttcta aaagctccgg attgattaac accagccgtt
    20521 cgtcacggtt ttccgaaaca tcaataacaa tgatacgttg atggatatca acctgggttg
    20581 ccgccaggcc aataccttct tctgcgtaca tcgtctcgaa catatcatcg acgatacgct
    20641 gtatttctgc attcacttct tctaccggtt tagcaacttt gcgaagccgc tcgtccggaa
    20701 tatgtaacac ttgcaaaact gacataaatc tccagagatg tgttcagggg ttagaaagat
    20761 tatttcttct attctagaca aatccccctc tgattgacag catcactgac caatcgcaaa
    20821 gattgctaag gctgcttatg gcagggagat aaggatggtc gatacagaaa tttggctgcg
    20881 tttaatgagt atcagcagct tgtacggcga tgatatggtc cgtatagctc actggctggc
    20941 aaaacagtcg caaattgatg cggttggatt gcagcaaaca gggcttacat tgcggcaggc
    21001 acaacgcttt ctttcatttc cgcgaaagag tatcgaaagc tcactttgtt ggttggagca
    21061 acccaaccat catttaatcc ctgcggacag cgaattttat cctcctcaac ttctggtgac
    21121 gacagattac cccggcgcac tgtttgttga aggagaactg cacgcgctgc attcatttca
    21181 gcttgccgta gtggggagtc gggcgcattc atggtatggc gagcgatggg gacggttatt
    21241 ttgcgaaact ctggcgacgc gtggagtgac aattacgagt ggactggcgc gtggaatcga
    21301 tggtgtggcg cataaagctg ccttacaggt aaatggcgtc agcattgctg tattggggaa
    21361 tggacttaat accattcatc cccgccgcca tgcccgactg gctgccagtc tgcttgaaca
    21421 tggcggagct ctcgtctcgg aatttcccct cgatgttcca ccccttgctt acaatttccc
    21481 acgaagaaat cgcattatca gtggtctaag taaaggtgta ctggtggtgg aagcggcttt
    21541 gcgcagtggt tcgctggtga cagcacgttg tgcgcttgag cagaggcgtg aagtttttgc
    21601 cttgccaggt ccaataggga atccgggaag cgaagggcct cactggttaa taaaacaagg
    21661 tgcgattctt gtgacggaac cggaagaaat tctggaaaac ttgcaatttg gattgcactg
    21721 gttgccagac gcccctgaaa attcatttta ttcaccagat cagcaagacg tggcattgcc
    21781 atttcctgag ctcctggcta acgtaggaga tgaggtaaca cctgttgacg tcgtcgctga
    21841 acgtgccggc caacctgtgc cagaggtagt tactcaacta ctcgaactgg agttagcagg
    21901 atggatcgca gctgtacccg gcggctatgt ccgattgagg agggcatgcc atgttcgacg
    21961 tactaatgta tttgtttgaa acctatattc acactgaagc tgagttgcgt gtggatcaag
    22021 acaaacttga acaggatctt accgacgcag gttttgagcg agaagatatc tacaatgccc
    22081 tgctatggct ggaaaaactt gctgattatc aggaagggtt ggcagaaccg atgcaactgg
    22141 cctcggatcc tctctccatg cgtatttata caccggaaga gtgtgaacga ctggatgcca
    22201 gctgccgtgg gtttctgctt ttccttgagc agattcaggt gctcaacctt gaaactcgtg
    22261 aaatggtgat agagcgagtg ctcgcgctgg ataacgcaga gttcgaactg gatgatctga
    22321 aatgggtgat cctgatggtg ttgttcaaca tcccggggtg cgaaaatgcg taccagcaaa
    22381 tggaagaatt actctttgaa gtgaatgaag gtatgctgca ttaatcattt ctttaattca
    22441 gcataagttg ttatggcaaa atcagcactg ttcacggtgc gtaataatga gtcctgccca
    22501 aagtgcgggg ctgaactggt tattcgatcc gggaaacacg gtccgtttct tggatgctca
    22561 cagtatccgg cgtgtgacta cgtccgtctt ctgaaatctt cagcggatgg acatattgtc
    22621 aaagttctgg aggggcaggt ttgccctgca tgtggcgcaa atctggtatt acgccaggga
    22681 cgctttggta tgtttattgg ttgcagtaac taccctgaat gcgaacatac cgaacttatc
    22741 gataaaccgg acgaaacagc aattacatgc ccccaatgtc ggacgggcca tctggtccag
    22801 cgccgctccc gttatggcaa aacatttcac tcctgtgatc gctacccgga gtgtcaattt
    22861 gccattaact tcaaaccaat agctggagaa tgccctgagt gtcattatcc gctactcatc
    22921 gaaaagaaaa ccgcgcaggg tgtaaaacac ttttgtgcca gtaaacaatg tggaaagccg
    22981 gtttcggcgg aataataacg tgaataataa cctgcaagga gacgctatcg cagctgcgat
    23041 agatgtcctc aatgaagaac gtgtcatcgc ctatccaacg gaagccgttt tcggtgttgg
    23101 gtgcgatcct gatagcgaaa cagcagtgat gagactgttg gagttaaaac agcgtccggt
    23161 tgataagggg ctgattttaa tcgcggcaaa ttacgagcag cttaaaccct atattgatga
    23221 caccatgctg actgatgcgc agcgtgaaac cattttttcc cgctggccag gtcctgtcac
    23281 ctttgtcttt cccgcgcctg cgacaacacc gcgctggttg acgggccgct ttgattcgct
    23341 tgctgtacga gtcaccgacc atccgttggt ggttgctttg tgccaggctt atggtaaacc
    23401 gctggtttct accagtgcca acttgagtgg attgccacct tgtcgaacag tagacgaagt
    23461 tcgcgcacaa tttggcgcgg cgttcccggt tgtgcctggt gaaacggggg ggcgtttaaa
    23521 tccttcagaa atccgcgatg ccctgacggg tgaactgttt cgacaggggt aacataatgg
    23581 aaacctatgc tgtttttggt aatccgatag cccacagcaa atcgccattc attcatcagc
    23641 aatttgctca gcaactgaat attgaacatc cctatgggcg cgtgttggca cccatcaatg
    23701 atttcatcaa cacactgaac gctttctttc gtgctggtgg taaaggtgcg aatgtgacgg
    23761 tgccttttaa agaagaggct tttgccagag cggatgagct tactgaacgg gcagcgttgg
    23821 ctggtgcagt taataccctc aagcggttag aagatggacg cctgctgggt gacaataccg
    23881 atggtgtagg cttgttaagc gatctggaac gtctgtcttt tatccgccct ggtttacgta
    23941 ttctgcttat cggcgctggt ggagcatctc gcggcgtact actgccactt ctttccctag
    24001 actgtgcggt gacaatcact aatcggacgg tatcccgcgc ggaagagttg gctaaattgt
    24061 ttgcgcacac tggcagtatt caggcgttgg gtatggacga actggaaggt catgagtttg
    24121 atctcattat taatgcaaca tccagtggca tcagtggtga tattccggcg atcccgtcat
    24181 cgctcattca tccaggcatt tattgctatg acatgttcta tcagaaagga aaaactcctt
    24241 ttctggcatg gtgtgagcag cgaggctcaa agcgtaatgc tgatggttta ggaatgctgg
    24301 tggcacaggc ggctcatgcc ttccttcttt ggcatggcgt tctgcctgat gtagaaccgg
    24361 tcatcaaact attgcaacag gaattgtcag cgtgaatcag gccatccagt ttccggatag
    24421 ggaagagtgg gacgagaata aaagatgtgt atgttttcct gctctcgtga atggtatgca
    24481 actgacatgt gcgatctctg gcgagagtct ggcgtatcgc tttactggag gtacgccaga
    24541 acagtggtta gcgagttttc gtcagcatcg ctgggacctg gaagaagaag cggaaaactt
    24601 aattcaggaa caaagtgaag atgatcaagg ttgggtctgg ttaccttgat ccagatattc
    24661 gtccttccat ttcacgtagt tatttgcgga atagcgtaac ccagccttct cttcatcact
    24721 taacgggcgg atctgtttga cgggactacc gagatacaga tatccgctct ccagccgttt
    24781 attttgtggg accaggctac ccgcaccaat catcacatca tcttctacta ttgcgccatc
    24841 aagtaaaatt gagcccatcc caaccaaaac tcgattgcca atggtgcagc cgtggagcat
    24901 caccttgtga ccaacagtga catcttcgcc aatggttaat gggttgccat ctgggttgta
    24961 cgaggattta tgagtgacat gcaacatact gccatcctgg atattggtgc gtgctccgat
    25021 ctgtacataa tgtacatctc cacgaatcac aacgagcggc cagatcccca catcatcagc
    25081 cagacgaacg tcaccaatca cgacgctgct atcgtcgatc attacgcgct ggccgatttg
    25141 tggaaaaaga tcgcggtatg ggcgtaaaac atcagacata ctcacctcaa caataaatga
    25201 tttcctaatg actttgggtg cattattagc cttgtgcaag tcttttagta tgcaaaaaag
    25261 caccgttttg tgtgcgaatg cagcaaaaag ggtgaaaaaa caacaaacag aaaaaaagat
    25321 caaaaaagta cttgtgcaaa aaattgggat ccctataatg cgcctccgtt gagacgacaa
    25381 cgtgaaacac ttcacaggat ggtcggaaca acgaagagaa aaaattctga aattcagggt
    25441 tgactctgaa agaggaaagc gtaatatacg ccacctcgcg acagtgagct gaaagccgcg
    25501 tcgcaactgc tctttaacaa tttatcagac aatctgtgtg ggcactcgaa gatacggatt
    25561 cttaacgtcg caagacgaaa aatgaatacc aagtctcaag agtgaacacg taattcatta
    25621 cgaagtttaa ttctttgagc atcaaacttt taaattgaag agtttgatca tggctcagat
    25681 tgaacgctgg cggcaggcct aacacatgca agtcgaacgg taacaggaag cagcttgctg
    25741 cttcgctgac gagtggcgga cgggtgagta atgtctggga aactgcctga tggaggggga
    25801 taactactgg aaacggtagc taa
//
LOCUS       DAEEKQ010000045        25805 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.045, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000045 DAEEKQ010000000
VERSION     DAEEKQ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25805)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25805)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25805
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.045"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="KLK57_001788"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="KLK57_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE0892172.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(286..2400)
                     /gene="fusA"
                     /locus_tag="KLK57_001789"
     CDS             complement(286..2400)
                     /gene="fusA"
                     /locus_tag="KLK57_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HBE0892173.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="KLK57_001790"
     CDS             complement(2497..2967)
                     /gene="rpsG"
                     /locus_tag="KLK57_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HBE0892174.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="KLK57_001791"
     CDS             complement(3064..3438)
                     /gene="rpsL"
                     /locus_tag="KLK57_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HBE0892175.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="KLK57_001792"
     CDS             complement(3564..3851)
                     /gene="tusB"
                     /locus_tag="KLK57_001792"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HBE0892176.1"
                     /translation="MLHTLHRSPWLADFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="KLK57_001793"
     CDS             complement(3859..4218)
                     /gene="tusC"
                     /locus_tag="KLK57_001793"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HBE0892177.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLPGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="KLK57_001794"
     CDS             complement(4218..4604)
                     /gene="tusD"
                     /locus_tag="KLK57_001794"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HBE0892178.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGVVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4604..5326)
                     /locus_tag="KLK57_001795"
     CDS             complement(4604..5326)
                     /locus_tag="KLK57_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0892179.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="KLK57_001796"
     CDS             complement(5493..6305)
                     /gene="fkpA"
                     /locus_tag="KLK57_001796"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE0892180.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6526..6744
                     /locus_tag="KLK57_001797"
     CDS             6526..6744
                     /locus_tag="KLK57_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HBE0892181.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="KLK57_001798"
     CDS             complement(6793..7383)
                     /gene="slyD"
                     /locus_tag="KLK57_001798"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE0892182.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7478..7678)
                     /locus_tag="KLK57_001799"
     CDS             complement(7478..7678)
                     /locus_tag="KLK57_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HBE0892183.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="KLK57_001800"
     CDS             complement(7688..9493)
                     /gene="kefB"
                     /locus_tag="KLK57_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HBE0892184.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="KLK57_001801"
     CDS             complement(9493..10044)
                     /gene="kefG"
                     /locus_tag="KLK57_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HBE0892185.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10175..12088
                     /locus_tag="KLK57_001802"
     CDS             10175..12088
                     /locus_tag="KLK57_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0892186.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12088..13110
                     /locus_tag="KLK57_001803"
     CDS             12088..13110
                     /locus_tag="KLK57_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE0892187.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13104..13322
                     /locus_tag="KLK57_001804"
     CDS             13104..13322
                     /locus_tag="KLK57_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HBE0892188.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13376..14245
                     /locus_tag="KLK57_001805"
     CDS             13376..14245
                     /locus_tag="KLK57_001805"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HBE0892189.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14300..14704)
                     /locus_tag="KLK57_001806"
     CDS             complement(14300..14704)
                     /locus_tag="KLK57_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HBE0892190.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15006..15638
                     /gene="crp"
                     /locus_tag="KLK57_001807"
     CDS             15006..15638
                     /gene="crp"
                     /locus_tag="KLK57_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HBE0892191.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15689..17779
                     /locus_tag="KLK57_001808"
     CDS             15689..17779
                     /locus_tag="KLK57_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HBE0892192.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDSPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="KLK57_001809"
     CDS             complement(17846..19066)
                     /gene="argD/dapC"
                     /locus_tag="KLK57_001809"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HBE0892193.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGEILWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINPPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQYKGRARDFLYAGAEAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="KLK57_001810"
     CDS             complement(19152..19715)
                     /gene="pabA"
                     /locus_tag="KLK57_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HBE0892194.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19747..20349)
                     /locus_tag="KLK57_001811"
     CDS             complement(19747..20349)
                     /locus_tag="KLK57_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HBE0892195.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSKAGESE"
     gene            complement(20339..20506)
                     /locus_tag="KLK57_001812"
     CDS             complement(20339..20506)
                     /locus_tag="KLK57_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HBE0892196.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="KLK57_001813"
                     /gene_synonym="rotA"
     CDS             complement(20611..21183)
                     /gene="ppiA"
                     /locus_tag="KLK57_001813"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HBE0892197.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21454..22635
                     /gene="tsgA"
                     /locus_tag="KLK57_001814"
     CDS             21454..22635
                     /gene="tsgA"
                     /locus_tag="KLK57_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HBE0892198.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22897..25440
                     /gene="nirB"
                     /locus_tag="KLK57_001815"
     CDS             22897..25440
                     /gene="nirB"
                     /locus_tag="KLK57_001815"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HBE0892199.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25437..25763
                     /gene="nirD"
                     /locus_tag="KLK57_001816"
     CDS             25437..25763
                     /gene="nirD"
                     /locus_tag="KLK57_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HBE0892200.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
BASE COUNT         6295 a         6985 c         6459 g         6066 t
ORIGIN      
        1 gtgtcatatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc acgagcagca ccgccgtagg ttttagccag tacggtggtg
      121 attgcagcgg tcagagtagt tttaccgtgg tcaacgtggc cgatagtacc aacgttaacg
      181 tgcggttttg tacgttcaaa tttttcttta gacacggcta tattccttac tatagtgctc
      241 tccccttcag gagagagcac gggactttgg tattaaccct taggcttatt taccacgggc
      301 ttcaattacg gcctgagcaa cgttactcgg cgcttcatca tacttcagga attccatagt
      361 gtatgatgca cgacctttgg tcagagaacg cagctgagtt gcgtatccga acatttcaga
      421 cagcggtact tcagcgtgga tcttaacgcc agtaacttca gattcctgac ctttgagcat
      481 accacgacga cggctcaagt caccgataac gtcaccggtg ttctcttccg gagtttctac
      541 ttcaaccttc atgatcggct caagcagaac tggtttcgct ttcttaaagc cttctttaaa
      601 ggcgatagaa gcagccagtt taaacgccag ttcagaggag tcaacgtcat ggtaagaacc
      661 gaagtgcaga cgaataccca tgtctactac cgggtagcct gccagcggac ctgctttcag
      721 ctgttcctgg atacctttat caacggccgg gatgtattcg ccagggatta caccaccttt
      781 aatgtcgttg atgaactcgt agcctttcgg gtttgaaccc ggctccagcg ggtacatgtc
      841 gataacaaca tgaccatact gaccacgacc accagactgt ttcgcgtgtt taccttcaac
      901 atcggtaact ttctggcgga tagtttcacg gtaagcaacc tgcggtttac ctacgttcgc
      961 ttcaacgttg aattcacgct tcatacggtc aacgatgatg tcgaggtgca gttcgcccat
     1021 acccgcgatg atggtctggt tagattcttc gtcagtccat acacggaaag acgggtcttc
     1081 tttagccaga cggcccagag ccagacccat tttttcctgg tcagctttgg ttttcggttc
     1141 aactgcgatg gagattaccg gctcagggaa ttccatacgt tccagaatga tcggcgcatc
     1201 cgggtcacac agggtgtcac cagtggttac gtctttcaga ccgatagcag cagcgatgtc
     1261 gcccgcgcga acttctttga tctcttcacg tttgttagcg tgcatctgaa cgatacgacc
     1321 gaaacgctca cgtgcagctt tcacggagtt cagtacggta tcaccagagt taaccacacc
     1381 ggagtaaaca cggaagaagg tcaggttacc aacaaacggg tcggtagcga ttttgaacgc
     1441 cagtgcagag aacggctcgt catcacttgc gtgacgctca gccggagtgt ctttaccgtc
     1501 gtccaggata ccgttgatcg caggtacgtc aaccggggat ggcaggtaat caattaccgc
     1561 atccagcatc gcctgaacac ctttgttctt gaacgcagaa ccacaggtta ccaggatgat
     1621 ttcgttgttc agaacgcgct gacgcagagc acctttgatt tctgcttcag tcagttcttc
     1681 accacccagg tatttttcca tcagctcttc agaagcttca gctgcggatt cgatcaggtt
     1741 ctggtgccat tcgttagcca gttcaaccat gtctgccggg atatcttcgt attcgaaggt
     1801 tacgccctgg tcagcgtcgt tccagttgat agctttcatt ttcaccaggt caacaacacc
     1861 ggtgaaatgt tcttcagcac caatcgccag ctgcagcgga accgggttcg cgcccagacg
     1921 ggttttgatc tggttaacaa ctttcaggaa gttcgcaccc atgcggtcca ttttgttaac
     1981 gaacgcaatg cgcggaactt tatatttgtt tgcctgacgc catacggttt cagactgcgg
     2041 ctgaacacca ccaactgcgc agtaaaccat taccgcacca tcgagaacac gcatggaacg
     2101 ttctacttcg attgtgaagt caacgtgccc cggggtgtcg atgatgttga tgcgatgcgg
     2161 ctcatactgc ttagccatac cagaccagaa tgcagtagtc gcagcggaag tgatggtaat
     2221 accacgttcc tgctcctgct ccatccagtc catggttgca gcgccgtcat gaacttcacc
     2281 gattttatgg tttacaccgg tgtagaacag aatacgttcg gtagtagtgg ttttaccggc
     2341 gtcgatgtgc gcactgatac cgatgttacg gtagcgtgcg atgggtgttg tacgagccat
     2401 ttgtttcctc gtttatcttt taggcgttca atttaagtag cccaaagcgg gctgcttact
     2461 ggaagcgccc gcctggtgac taaaactccg aagggattac caacggtagt gtgcgaacgc
     2521 cttgttggct tcggccatac ggtgaacgtc ttcacgtttc ttaactgcag tacctttgtt
     2581 ttctgcagca tcagaaagtt cgttcgccag gcgcagagcc atggatttat caccgcgttt
     2641 acgagcagct tcaacgatcc aacgcattgc cagagcatta cgacgaaccg gacggacttc
     2701 aactggtacc tgataagtag aaccaccaac gcggcgagac ttaacttcta cagtcgggcg
     2761 cacgttttcg agagctactt cgaatgcttc cagttcagat ttaccagagc gctgagccag
     2821 ggtctccagc gcgctgtata cgatagattc agcagtagat tttttaccat ctaccatcag
     2881 gatatttaca aatttagcca gcagttctga tccaaacttc ggatccggca gaattttacg
     2941 ctgaccaatg acgcgacgac gtggcatgga aatactccgt tgttaattca ggattgtcca
     3001 aaactctacg agtttagttt gacatttaag ttaaaacgtt tggccttact taacggagaa
     3061 ccattaagcc ttaggacgct tcacgccata cttggaacga gcctgcttac ggtctttaac
     3121 gccggagcag tcaagcgcac cacgtacggt gtggtaacga acacccggga ggtctttaac
     3181 acgaccgcca cggatcagga tcacggagtg ctcctgcagg ttgtgacctt caccaccgat
     3241 gtaggaagtc acttcgaaac cgttagtcag acgaacacgg catactttac gcagcgcgga
     3301 gttcggtttt ttaggagtgg tagtatatac acgagtacat acgccacgtt tttgcgggca
     3361 tgcttccagc gcaggcacgt tgcttttcgc aactttgcga gcacgtggtt tgcgtaccag
     3421 ctggttaact gttgccatta aatagctcct ggttttagct tttgcttcgt aaacacgtaa
     3481 taaaacgtcc tcacacaata tgaggacgcc gaattttagg gcgatgccga aaagatgtca
     3541 agaaatatac aacgatcccg ccatcaccag gccatctggc tggagtgctt aaccgtaagt
     3601 ctgacgaaat cagtatagtc aatgggaatg atgtcgttcg aaatttgacc agtcaaaccg
     3661 cgggcaataa ggtcttcgtt cagggcatag accttaatgg gggcattacg cagactttca
     3721 aggtagcggt taccgtcaac tgcggcagtt acgccatctt gcaataatag cagttcgtcg
     3781 ccttcactga gcagacgcag cagcgcagca aaatccgcca gccagggtga acgatgtaat
     3841 gtgtgcagca taaaagcctc aaaacctcaa aataacatcg tagttggcga gttcgcggcg
     3901 taaggcatct gcttcgagcg gcgtggcttc gacaacaaag ggggtctgcg gatctaaccc
     3961 gcgttcgcgc agtgaagccg cacaaaccca gcactgttca atgtcgtaca gacccaacaa
     4021 tttaaaagtg gcaatgtaat cacgcgccag cactgcatcg ggcttttgtc ctggcagcag
     4081 ctgaaaaacg ccatcagcta taaagaagac agccagatca tcagttaatg cggaagttgc
     4141 cagtaaagca tctaaacctt cccgaccagc agctgtacca tgaggcgcag tagaaaaaac
     4201 aaacgcaatt cgtttcatca gaactgtacc accctgtcac aggtcagcga ggcttccgcc
     4261 agcgcgccaa gtccgcttaa ggtaaatccc ggctgaaggt ttgacgaggc cagccccagc
     4321 cttccggcct ccgtttcatc aacaacgcca cggcgtaatg ctgccgctac gcagatattc
     4381 agcgccacac catgttgcgc attcaactgt tgccagccgc gcacaaggtc aaattcgtcg
     4441 cttgccggtg aggtcaattg gttagcgtta tagacccctt cccgataaaa aaagacgctg
     4501 cttaactcat ggccttctac tatcagcgcc tgcgcaaact gaaaagcact actcgcctgt
     4561 tgcgtaccgt atgctggccc ggtcaccacg atggcaaaac gcattactta tcttgcccct
     4621 ggaaatcacc gcttttgaac tggcggatgt agagatagac agtgtgtttg gagatgttca
     4681 ggcggtcagc aacctggttg atcgcgtctt taatatcgaa gatccctttc tcgtagagat
     4741 tcagcacgat ctgacgattt ttggcgttat tagaaacatt gcgatcggca tttacttctt
     4801 cgatggtgaa ctccagcgtt tgggtaacca gatcttcaac agaagaggca aagttgacgc
     4861 ttgaaccgac atccggggtt tctggcggca caaaagtgct cataatctgc gagaagggaa
     4921 catcaagatt catattgatg cacagcagac caattacacg ctgttcgcgg ttacgaatcg
     4981 cgatagtcag ggacttcatt aatacgccgc ttttggcgcg agtaaagtag catttagaaa
     5041 cgctgctatc cgctcccgtc atatcgtgca gcatacgtag cgccaggtca gtaattggcg
     5101 aaccaatctt ccggcctgta tgttcaccgt tagcaatgcg aatggctgaa cattttagat
     5161 cctgcaaaga gtgcaaaacg atttcacagt gggagccaat aagcatcgct aacccgtcca
     5221 ccaccgcttc gtaggatttc agaatatcaa aatcggtctg gtcgaaagga cgttgatcca
     5281 gtaaatccaa ctcactggtt tcgttggtta aaagcgacct ggacatgaaa aaaaacactc
     5341 cttttcagga gcctgtcgtt aacttttcag ggcaggctca ttaatgatgc gggtaactaa
     5401 attaatacag cggaggttcc gctttccagc actaattata tccggcctgt aataaaaaaa
     5461 ccgccgcctg gtcaggcggc ggttcttaat gcttattttt tagcagaatc tgcggctttc
     5521 gcatcagctt ccggctttgc atcagccttc ggcgctggtt tcacatccag cagctctacg
     5581 tcaaacacca gggtagaatt cggtgggatc cccggaacac ccgctttgcc gtaagccagt
     5641 tctggtggaa taaccagttt gatcttaccg cctttcttga tgttcttcag accttctgtc
     5701 caccccggga taacaccgtc cagacggaaa gagagcggtt caccacgggt gtaagagttg
     5761 tcgaactctt taccgtcgat cagcgtacct ttgtagttca ctacaacagt atcgctgtct
     5821 ttcggtgctt cgcctttacc ggcttctact acctgataaa ccagaccagt tgaagaggtt
     5881 ttcacacctt tctctttggc aaatttctcg cggtactctt taccttttgc ttcgttatca
     5941 gccgcgtctt tttccatctt cgcctgagca gaagacttca cgcgagcttc gaatgcttgc
     6001 agagtctgtt cgatctcttg gtcggagagt ttgctcttat cagcaaatgc atcctgaaca
     6061 ccagcgatca gctgatcttt atccagtttg atgcccagtt tttcttgttc tttaagagag
     6121 ttttccatgt aacgacccag cgaagcaccc agtgcataag ctgatttctg atcgtcattt
     6181 ttgaacgctg ctttgctgtc agcagttgta gcaggttttg cagcttcagc agcaaaagtg
     6241 attggtgcat gcagggcaac ggccattgtg gtcgccagca gcgttacttt aaacagtgat
     6301 ttcatccata tctccaggat cggggcatct caccccaggg ttaactatta tcagaagggt
     6361 actataaagc gttgtcgaac aaatctacat acagacacgc cctattatca tctattttca
     6421 gactcttttt gtttaaatta gtttcgatga ccgcgaaatg agtgctgtct cgggcagcaa
     6481 agttaagtag aatccgcggc gaccattcga caaaagaggt gaatcatgca ggatttatca
     6541 ttggaagcac gcctggcaga gctggagagc cgactggctt ttcaggaaat caccattgaa
     6601 gaactgaacg tcacggtgac cgctcatgaa atggagatgg cgaaactgcg cgatcatctg
     6661 cgtctgctga ccgagaagtt aaaagccagc cagccgtcga acatcgcgtc gcaggctgaa
     6721 gaaacgccac cgccacatta ttgaggcgta aaaaaagcgg ggattccccg cttttttgtc
     6781 accttttcgg tattagtggc aaccacaacc gccgttgcct ttaccgccac agcagccttc
     6841 gccaccgtgt tcatgaccgt gatcgtggcc atgaccgccg cagcaaccgt cgtggtcgtg
     6901 atcgtggtga tgatcgtgcg cgccgtgaac gtgaccatga gccagttctt cttcggtcgc
     6961 ttcacgaatc gccacaacct caacgttgaa tttcaggttc tgaccggcca gcatgtggtt
     7021 accatcaacc actacgtgat cgtcttcaac cgcagtgatt tcaaccggta ccggaccctg
     7081 gtcggtttca gccaggaaac gcatacctac ctgcagttca tcaacgccca taaatacgtc
     7141 tttaggaaca cgttgcacca ggttttcgtc gtactgaccg taagcgtcgt tcgcgccaac
     7201 agcgacatca aatttgtcgc caacttcatg accttccagc gccgtttcca ggccagagat
     7261 cagggaaccg tgaccatgca ggtagtccag cggcgcactc accggagact catcaaccaa
     7321 cacaccgtct tctgtacgta cctgataggc caggctgacc accaggtctt ttgctacttt
     7381 catgatatct cctgagcatg ggaagaatag tggcgcagat tgtagcggaa ttctgcagcc
     7441 gtgtactcac tagcttaaaa aaactcggcg catatcgcta gtccggatga aaaatcccga
     7501 tcacttgctc atctttgcga acgtgatcgc gggcttcttt gtctgcttct cgcatctgat
     7561 gtccgcactt aacgcattca acaatatcaa tattattttc gcgccacatc gccattgaat
     7621 cctgtgcctg acaggccggg cattttgcgc ccgcaataaa acgttttcgg attgccatct
     7681 ttaccctcta ctcaaattca tcccagccgt ccagctggcg tcgttcttgt tgcatttcac
     7741 gctggaaaat ctcttccagt tcgcgtcggg cttccctggc gcgagaaatt tgtacagtat
     7801 cggcatgcat tgggatgagc tctcgcagca tccgcatatc caggcggcga aaatgcagtt
     7861 gcgcccgctg cgcctgatgc ggatgcatcc caagcgtgac caaagtcttg cgccccagct
     7921 ctaacgcact ggagaatgtt tcacgggaaa actgcgtcac ccctgcctgt aataactcat
     7981 gcgcttccac acgtccgcgc gctcgcgcaa gaatatgcaa atgcggaaag tgctgctggc
     8041 atatttccac cagcttcatg gtgtcttccg gctcgttaca ggtaatgacg atagactcag
     8101 cggcctctgc acccgcagaa cgtaatagat cgacctgcgt ggcgtcgccg taataaactt
     8161 tgtagccata tttacgcatc aggttaacgg cgctgatatc ccgctccagc acggtaatgc
     8221 gcattttatt tgccatcagc aaacgaccaa tcacctgacc aaaacgcccg aagcccacga
     8281 caatgacctg gggtttatca tcgttgaccc acggtttttc gtcttcttct tccggtccgt
     8341 taaactggcg ggatagccat ttatccacca gcttcatcag caacggcgtg gtcatcatgg
     8401 aaagcgtcac cgtcaccagc aacagcgcca tctggtcgcc ctggaataaa cgttgtgaag
     8461 aagcggtaga aaagaggaca aaggcaaact caccgccctg actcaacacg ccagcaaact
     8521 gcatccgctc tgagctacgc acgccataca atcgcgccag cagatacagc acgagaattt
     8581 tcaccgccac cagcacaacc acgcttatca ctacccacaa cagatgggta taaagcaccc
     8641 cgaggttgag cgacatgccg acagagataa agaacaaacc gagcagcaag cctttgaagg
     8701 gatcgatagc cgtttccagt tcatggcgat attcactttc cgccagcagc acgcccgcaa
     8761 taaatgtacc gagcgccatc gacagcccca gcgcatccat aaacaatgcg gaacccaaca
     8821 ccagcagcag cgtcgcggcg gtgaacactt cccgcacgcc agaagctgca ataaagcgga
     8881 ataccggacg cagtaaatag cgcccaccaa tcagcatgcc gacaaacgcc agcaccttca
     8941 tgccgatctt catccagtcg aaatgttcgt ctgccgaccc cgccaacaac ggcactaacg
     9001 ccagtgctgg gatcactgcc agatcctgaa acagcagaac cgaaaacccg agctggccgg
     9061 attcgctgcg attcatccct ttctcacgca tcaattgcaa cgccattgca gttgaagaca
     9121 tcgcaaggcc aatgccaccg accaccgccg cctgccaggc gaaatccgtc agcatcaata
     9181 atcccgccag caacgccgcg cttaacagca cctgtgccgc gcctacgcca aaaatcgaac
     9241 gccgcagttg ccaaagtttg gagggattca actcaaggcc gatgataaac atcaggaata
     9301 ccacgccgag ttccgaaaag tggaggatct catcgacgtc gctaataaac cccagccccc
     9361 acgggccaat tgcaatccct gccagcaaat atcccaacac agcgccaatc cccagccgcg
     9421 atgccaacgg caccgcagcc accgccgcga agagaaacag cactcctgcg agtaaaaaat
     9481 cggaaccttc catcagcggc ctcctggaga cagcggattt gccagccagt caccgtaggc
     9541 tctggcgtgg ctcgccagct cctttgcgct ttgccgtctc gcccagtaaa taatgatggg
     9601 acttaaccaa tgcatccggc acatgcccgc cgccagttca aaggggcgca gcacatcgct
     9661 catcgggtag cgattcagcg cgtcataacg gtaagcactt tccggctcgc cggtggtaat
     9721 cacgttacgc cagtactttc ccgccagttg gtttcctccc ggcccgctgg caaaaccacg
     9781 acttaatacc cggtccagcc actctttcag tagcgccggg cagctatagg tataaagagg
     9841 atgctgaaag acaatcacct cgtgctcgcg cagtaatgcc tgctcacggg ggatatcaat
     9901 aaaaaaatcg ggatagtgcg cgtaaaggtc gtgcacggta acattgctga gctgcgtggc
     9961 cggtttaagc agtacccggt ttgccaccga gtcctgagat tccggatggg catacagcag
    10021 caaaactttc gctggctgag acatcatccc cctcccggta tggtttttgt gtatagtcgc
    10081 tgttttgggc taccattgcg cccggtgcgg cagctcgccc atacattaca ttatcataat
    10141 gataagttaa catagtctga acatacggcg ccttatgatt gttttctcct cgttacaaat
    10201 tcgtcgcggc gtgcgcgtcc tgctggataa tgccaccgcc accatcaacc ccgggcagaa
    10261 agtcggcctg gtgggtaaaa acggctgtgg taaatctacc ctgctggcat tgctgaaaaa
    10321 tgaaatcagc gccgacggcg gcagctacac ctttccggga agctggcaac tggcgtgggt
    10381 gaatcaggaa acgccagcgt taccgcaagc ggcgctggaa tatgtcattg acggcgaccg
    10441 tgaatatcgt caactggaag cgcagctgca cgacgccaac gaacgtaacg acgggcacgc
    10501 cattgcgacc attcatggca agctggatgc tattgacgca tggagtattc gctcccgtgc
    10561 cgccagcctg ctgcacggcc tcggtttcag caatgaacaa ctggagcgcc cggtaagtga
    10621 tttctccggt ggctggcgta tgcgtcttaa ccttgcccag gcgctgattt gccgttcaga
    10681 cttgctgctg ctcgacgaac cgactaacca cctcgatctc gatgccgtta tctggctgga
    10741 aaaatggttg aagagctatc agggcacgct gatcctgatc tctcacgacc gcgacttcct
    10801 cgatccgatc gttgataaaa ttattcatat cgaacaacaa agcatgttcg agtacaccgg
    10861 caactacagt tcgtttgaag tacagcgcgc cacccgtctg gcgcagcaac aagcgatgta
    10921 tgaaagccag caggaacgcg tggcacatct gcaaagttat atcgaccgtt tccgtgccaa
    10981 agccaccaaa gcgaagcagg cccagagccg cattaagatg cttgagcgta tggagctgat
    11041 tgcccccgcg cacgtcgaca acccgttccg ctttagcttc cgcgcgccgg aaagcctgcc
    11101 aaatccgtta ctgaagatgg aaaaagtcag cgcgggctat ggcgatcgca ttattctcga
    11161 ctcgattaaa ctgaacctgg tgcccggctc gcgcattggt ctgttaggcc gcaacggcgc
    11221 gggtaaatcg acattaatca aactgttagc cggtgaactt gcgccagtca gcggtgaaat
    11281 tggtctggcg aaagggatca agctcggcta cttcgcccag catcaacttg aatacctgcg
    11341 cgccgacgaa tcacctattc aacatctggc acgtttagcg ccgcaggagc tggaacaaaa
    11401 actgcgtgac tacctcggcg gctttggttt ccagggcgat aaagtaaccg aagaaacgcg
    11461 ccgcttctcc ggtggggaaa aagcccgcct ggtgctggca ttaattgtct ggcagcgtcc
    11521 gaatctgctg ctgctcgacg aaccgactaa ccaccttgac ctcgacatgc gtcaggcact
    11581 caccgaagca ttaatcgagt ttgaaggcgc gctggttgtc gtttcgcacg accgtcattt
    11641 gctgcgttcc accactgacg atctctacct ggttcacgat cgtaaagtcg aaccgttcga
    11701 cggcgatctg gaagattatc aacagtggtt gagcgacgta caaaagcagg aaaaccagac
    11761 cgacgaagcg ccaaaagaga acgcgaacag cgcccaggca cgtaaagatc agaagcgtcg
    11821 ggaagcggag ctgcgtgcgc aaacccagcc gctgcgtaaa gagattgccc gtctggaaaa
    11881 agagatggag aagctgaacg cgcaactggc gcaggcggaa gagaaactcg gagacagcga
    11941 actgtatgac cagagccgta aagcggagtt gaccgcctgc ctgcaacagc aagccagcgc
    12001 caaatccggc ctggaagagt gcgaaatggc gtggctggaa gcccaggagc agcttgagca
    12061 gatgctgctg gaaggccaaa gcaactgatg gcgcagataa cgacgaccga tgccaatgaa
    12121 ttcagcagca gtgctgaatt cacccctatg cgcggcttta gcaattgtca tctacaaact
    12181 atgctgccgc gtttgtttcg tcgtcaggtg aaattcaccc cgtactggca gcggctggag
    12241 ttgcccgacg gcgattttgt cgatctcgcg tggagtgaag cccctgcaca ggctcggcat
    12301 aaaccgcgtt tagtggtatt tcacgggctg gaaggcagtc tcaatagccc ttacgcccac
    12361 ggtctggttg aggcggcgca aaagcgcggc tggctgggcg tggtgatgca ttttcgcgga
    12421 tgcagcggtg aaccaaaccg tatgcaccgc atttaccatt cgggcgaaac cgaagacgcc
    12481 agttggtttt tacgctggct gcaacgcgaa tttggtcatg cgccaacggc tgccgtcggc
    12541 tattcgctcg gcggtaatat gctggcctgt ttgctggcaa aggaaggcaa tgatctcccg
    12601 gttgatgcgg cggtgattgt ctctgcgccg tttatgctgg aagcctgtag ctatcatatg
    12661 gaaaagggct tttcccgcgt ttatcagcgt tacttgctga acctgttaaa agccaatgcc
    12721 gcgcgcaagc tggcagccta ccccggaacg ctgccgatta atctcgcgca gttaaaatcg
    12781 gtacgtcgca tccgtgaatt tgacgatcta atcaccgcca gaattcacgg ctacgccgac
    12841 gctatcgact attatcgtca gtgtagcgcc atgccgatgc tgaaccggat cgccaaaccg
    12901 acgctgatta ttcacgccaa agacgatccg tttatggatc atcaggtgat cccgaaaccg
    12961 gaaagtctcc ccccgcaggt ggagtatcaa ctgactgaac atggcggtca tgttggcttt
    13021 attggcggta cattacttca tccgcaaatg tggctggagt cacgcattcc tgactggtta
    13081 acaacgtatc tggaggcgaa atcatgttga ttccgtggca agacctctcc cccgaaacgc
    13141 tggaaaattt aattgaaagc tttgtgttac gtgaaggcac cgattatggt gaacatgagc
    13201 gtacacttga acagaaagtc gccgacgtca aacgccagct acagtgcgga gaagcggtgc
    13261 tggtatggtc ggagctgcac gaaacggtca atatcatgcc gcgcagtcag tttcgcgaat
    13321 agcctgcaac cgtcggcgga cgagataata atgatagcta agtcatggag ttactatgtc
    13381 tgccaaacat ccggtcattg cggtaacagg atccagcggc gcggggacca ccaccaccag
    13441 cctcgcgttt cgtaaaatat tcgcgcagtt aaatctgcat gcagctgagg tggaaggcga
    13501 cagttttcac cgttacaccc gcccggaaat ggacatggcg atccgcaaag cgcgcgacgc
    13561 cgggcggcat atcagctact tcggccccga ggctaacgac ttcggcctgc tggaacaaac
    13621 cttcattgaa tacggtcaga gcggcaaagg gaaatctcgc aaatatctgc atacctacga
    13681 cgaagccgta ccgtggaatc aggtaccggg gacattcacc ccctggcaac ctttaccgga
    13741 acccactgat gtactgtttt atgaaggttt acacggcggc gtagtcacgc cacagcataa
    13801 cgttgcgcag catgtggact tactggtcgg cgtggtgcct atcgttaacc ttgagtggat
    13861 tcaaaaactg atccgcgaca ccagcgagcg cgggcactca cgagaagcag tgatggactc
    13921 agtagtgcgt tcaatggaag actatatcaa ctacatcaca ccgcagtttt cccgcaccca
    13981 tcttaacttc cagcgcgttc ccaccgtcga cacttcaaac ccgttcgcgg caaaaggtat
    14041 cccgtcgctc gatgaaagct ttgtggtgat ccattttcgt aatctggaag ggatcgattt
    14101 cccctggctg ctggcgatgt tgcaaggctc attcatttcc cacatcaata cgttagtggt
    14161 accgggcggc aaaatgggtc tggcaatgga attaattatg ctgccgctgg tgcaacgatt
    14221 gatggaagga aagaaaatcg agtaactctg ctattacgcc ggataaaata ctatccggct
    14281 tcacaacggg atagttaaat cacgcggcaa ccacttcata cgagtgagta atattcaccg
    14341 ctttttccag catcaacgcc actgaacaat atttctcagc agagagatca accgcacgcg
    14401 caaccgctgc gtctttcagg tcgcgaccgg tgacgataaa atgcagatta atgtgcgtaa
    14461 acaggcgtgg tgcctcttcg cggcgttcag aggtcaattt tacttcacaa tcgaccacat
    14521 cctgacgccc tttttgcagg atagaaacca catcgatggc actgcaacca cccgccgcca
    14581 tcaacaccat ttccatcgga cttggtgctt tatcgcctga gttgccgtcc attaaaatct
    14641 ggtgaccaga ggcggattcg cccagaaaag ttaacccttc gacccacttc actcgcgctt
    14701 gcatttttgc tactccactg cgtcaatttt cctgacagag tacgcgtact aaccaaatcg
    14761 cgcaacggaa ggcaacctgg gtcatgctga agcgagacac caggagacac aaagcgaaag
    14821 ctatgctaaa acagtcagga tgctacagta atacattgat gtactgcatg tatgcaaagg
    14881 acgtcacatt accgtgcagt acagttgata gccccttccc aggtagcggg aagcatattt
    14941 cggcaatcca gagacagcgg cgttatctgg ctctggagaa agcttataac agaggataac
    15001 cgcgcatggt gcttggcaaa ccgcaaacag acccgactct cgaatggttc ttgtctcatt
    15061 gccacattca taagtaccca tccaagagca cgcttattca ccagggtgaa aaagcggaaa
    15121 cgctgtacta catcgttaaa ggctctgtgg cagtgctgat caaagacgaa gagggtaaag
    15181 aaatgatcct ctcctatctg aatcagggtg attttattgg cgaactgggc ctgtttgaag
    15241 agggccagga acgtagcgca tgggtacgtg cgaaaaccgc ctgtgaagtg gctgaaattt
    15301 cgtacaaaaa atttcgccaa ttgattcagg taaacccgga cattctgatg cgtctgtctg
    15361 cacagatggc gcgtcgtctg caagtcactt cagagaaagt gggcaacctg gcgttcctcg
    15421 acgtgacggg ccgcattgca cagactctgc tgaatctggc aaaacaacca gacgctatga
    15481 ctcacccgga cggtatgcaa atcaaaatta cccgtcagga aatcggtcag attgtcggct
    15541 gttctcgtga aaccgtggga cgcattctga agatgctgga agatcagaac ctgatctccg
    15601 cacacggtaa aaccatcgtc gtttacggca ctcgttaatc ccgtcggagt ggcgcgttac
    15661 ctggtagcgc gccattttgt ttcccccgat gtggcgcaga ctgatttatc accccgatat
    15721 caactatgca cttcgacaaa cgctggtgct atgtttgccc gtggccgttg ggttaatgct
    15781 tggcgaatta cgattcggtc tgctcttctc cctcgttcct gcctgttgca atattgcggg
    15841 ccttgatacg cctcataaac gttttttcaa acgcttaatc attggtgcgt cgctgtttgc
    15901 cacctgtagc ttgctgacac agctactact ggcaaaagat gttcccctgc cctttttgct
    15961 gaccggatta acgctggtac ttggcgtcac tgctgagctg gggccattgc acgcaaaatt
    16021 gcttcctgca tcgctgctcg ccgccatttt taccctcagt ctggcgggat acatgccggt
    16081 ctgggaaccg ttgctcatct atgcgttggg cactctctgg tacggattgt ttaactggtt
    16141 ttggttctgg atctggcgcg aacaaccgct gcgcgagtca ctaagtctgc tgtaccgcga
    16201 actggcagat tattgtgaag ccaaatacag cctgcttacc cagcacaccg accctgaaaa
    16261 agcgctgccg ccgctgctgg tgcgccagca aaaagcggtc gatctaatta cccagtgcta
    16321 tcagcaaatg catatgcttt ccgcgcaaaa taatactgat tacaagcgga tgctgcgtat
    16381 tttccaggag gcgctggatt tacaggaaca tatttcggtc agtttgcatc agccggaaga
    16441 ggtgcaaaag ctggtcgagc gtagccatgc ggaagaagtt atccgctgga atgcgcaaac
    16501 cgtcgccgct cgcctgcgcg tgctggctga tgacattctt taccatcgcc tgccaacgcg
    16561 ttttacgatg gaaaagcaaa ttggcgcact ggaaaaaatc gcccgccagc atccggataa
    16621 tccggttggg caattctgct actggcattt cagccgcatc gcccgcgtgc tgcgcaccca
    16681 aaaaccgctc tatgcccgtg acttactggc cgataaacag cggcgaatgc cgttacttcc
    16741 ggcgctgaaa agttatctgt cactaaagtc tccggcgcta cgcaatgccg gacgactcag
    16801 tgtgatgtta agcgttgcca gcctgatggg caccgcgctg catctgccga agtcgtactg
    16861 gatcctgatg acggtattgc tggtgacaca aaatggctat ggcgcaaccc gtctgaggat
    16921 tgtgaatcgc tccgtgggaa ccgtggtcgg gttaatcatt gcgggcgtgg cgctgcactt
    16981 taaaattccc gaaggttaca ccctgacgtt gatgctgatt accaccctcg ccagctacct
    17041 gatattgcgc aaaaactacg gctgggcgac ggtcggtttt actattaccg cagtgtatac
    17101 cctgcaacta ttgtggttga acggcgagca atacatcctt ccgcgtctta tcgataccat
    17161 tattggttgt ttaattgctt tcggcggtac tgtctggctg tggccgcagt ggcagagcgg
    17221 gttattgcgt aaaaacgccc atgacgcttt agaagcctat caggaagcga ttcgcttgat
    17281 tcttagcgag gatccgcaac ctacgccact ggcctggcag cgaatgcggg taaatcaggc
    17341 acataacact ctgtataact cattgaatca ggcgatgcag gaaccggcgt ttaacagcca
    17401 ttatctggca gatatgaaac tgtgggtaac gcacagccag tttattgttg agcatattaa
    17461 tgccatgacc acgctggcgc gggaacaccg ggcattgcca cctgaactgg cacaagagta
    17521 tttacagtct tgtgaaatcg ccattcagcg ttgtcagcag cgactggaat atgacgaacc
    17581 ggggagttct ggcgatgcca atatcatgga ttcgccggag atgcagccgc acgaaggcgc
    17641 ggcaggtacg ctggagcagc atttacagcg ggttattggt catctgaaca ccatgcacac
    17701 catttcgtcg atggcatggc gtcagcgacc gcatcacggg atttggctga gtcgcaagtt
    17761 gcgggattcg aaggcgtaat gcaggctaaa ccatcggata aggcattcac gccgcatccg
    17821 acatcttttg cctgatgctt cgtatttacg ccccaaccac cttcgccacc gcgtgggcga
    17881 aacgttgcat cccttcatcg atatccgcat cttccaccac cagcgacggt gcaaaacgca
    17941 tcacatccgg tccggcattc agcaccatta cgccagcctc tgcgcccgca tacaggaaat
    18001 cacgcgcccg acctttgtac tgtggtttca gctctgcgcc aatcaacagc cccataccgc
    18061 gaatatcgct aaacacatcg tactgctgat cgatcttctg cagatggtca acaaaacgct
    18121 ggcgtttcgc ctgaatgcct tccagcactt cagggggatt gatgatatca aacgccgccc
    18181 ccgctactgc acaggccaga ggattaccgc cgtaggtgga accgtgagaa ccaggatgaa
    18241 acgcagaagc aatttccgcc gtggtcagca tggcgctaat cgggaagccg ccgcctaacg
    18301 ctttcgcaga ggtcagaata tccggcgtca cgccgtagtg catgtaagca aacaaatcgc
    18361 cggtccgccc catcccgcac tgcacttcat caaacaccaa taatgcctga tgttgatcgc
    18421 acagctcgcg caagccctgc aaaaactctg gcgtcgcagc cgtcacgccg ccctcgccct
    18481 ggatcggctc aaccaccacc gcacaggtgt gatcatccat cactgctttc actgcgtgga
    18541 gatcgttaaa gggaacgtgg atgatgtctg ccggtttcgg cccaaagccg tcggaatatt
    18601 ttggctgccc acccaccgaa acggtaaaca gcgagcgacc atgaaaagcg ttatggaagg
    18661 caataatttt ggttttgaac gggctatgac gcacacaggc gtaatgacgt gccagtttaa
    18721 aggcggtttc gttagcttcc gtgccggagt tcataaacac cacgcgttcg gcaaacgttg
    18781 cctcaatcag tttacgccca agacgcagcg ccggttcatt ggtgaaaacg ttactgatat
    18841 gccacagaat ttcgccctgg gtttttaacg cgttcaccag cgcaggatgg caatggccca
    18901 acgccgtaac tgcaatgcca cccgcgaaat cgacatactc cttgccttgc tgatcccaga
    18961 ttcggctgcc ctgacctttt accggaataa actctgccgg agcataaatc ggcaggatca
    19021 cttcatcgaa agtcgcgcgt gtaattgctg tttgttcaat tgccatctca tgatcaccct
    19081 gttacgcata aacaaatgtg aaattataat cacaaaatat gcataaaaaa tcactaaatg
    19141 gcaatcagaa atcagcgatg caggaaatta gccagcagtt gatgtccttg ttcgctaaga
    19201 atactttctg gatggaactg cacaccttcc agatcccact ggcgatggcg aatccccata
    19261 atctcgcggg tttcgctcca ggccgtcacg tcaaagcacg ctggtaatga gtcaggttcc
    19321 accaccagcg aatggtagcg tgtcacggta agtggatttg ccagcccccg aaatacgccc
    19381 tcaccgctat gtgtaatcgg cgaggttttg ccgtgcatga cctttgcggc gcgcacaact
    19441 ttgccgccaa atgcctgcgc cattgcctga tgaccgaggc agacgccaag aatcggcaag
    19501 cgcccggcat agtgacgaat aacgtcaagg gagatcccgg cttcatctgg cgtacagggg
    19561 ccaggtgaga tgacaatttt ttgtggttta agggcgtcga tatccgccag cgtcaacgca
    19621 tcgttgcgct taaccagcac atccgccccc agttcacaaa agtactggta gaggttccag
    19681 gtaaaagaat cgtagttatc tataagcagg atcatggcgg ctccggtacg aaagaaccgc
    19741 gctattttac tcagattccc cggctttgct taccactttg ctaaatatca tctgcagtgc
    19801 ggtgagatcc cccattgccc cactctgatt tgcctgattc caggcctctt tttcgatacc
    19861 ctgccagctc agttgatatc cggcatgaat cgccagttgc tcgaagaaga tccgctgtgc
    19921 cagaccactt cccacccgga agggatgcag cacgttgatt tcacaatagt aatgcgccag
    19981 ccgctcgacg aacttcgctt tctccaggcc aaccagataa ccttcttcct ccagatcctg
    20041 catcagggca ttgccctctt tttcgatata agcaaagtgg cagaacggcg tatcgccctg
    20101 ataaatatca acttcacgca gttgccctgc ccagtcgaaa atatcctggt acagctggcg
    20161 atggatagtg cgcaaatgcg gtaaaccgcg caccagcgga ccaagctcaa tggtcgcagc
    20221 acgcagcgcc gtcatttcgt aagcggcctg ttccagccgc tgctgctgac ggatgttcag
    20281 ccggttacgc atgatatcaa ggccaggata aagatacgga tcgcgccctt caccgaattt
    20341 atcgctcata gtgcctcctc agctcttcaa ggcgcgctaa agcctctgcg gcagtaagag
    20401 tgactaaagg catctcgacg ccttcaagac ggcgactggc ctggaaatta cgattacgct
    20461 gaagctccca gagacgggac ttttgcttat cggtgagttt cttcacttga tgcctccctg
    20521 aacgtgtcca tttgccacaa gtataagcgg caaatccggg ttacgccggg agagcagaag
    20581 cgcgggcaag attgcccgcg cgagaaatca ttacggcagg actttagcgg aaaggataac
    20641 taccggtttt gacggcacat tctggtacgg accaacatca tgagtcggca cctgggaaat
    20701 cttatcggca acgtccatgc ctttcaccac tttaccaaat accgcgtaac cgaaatcacg
    20761 ctgaccatgg tcaaggaagg cgttatcggc aacgttgata aagaactggc tggtggcgct
    20821 gtctttgtca gcggtacgcg ccatcgcgat ggtgccacgc gtgttgcgca ggccgttatc
    20881 ggcttcattt ttgattggcg ggtttggttt tttctgctgc atctgctcgg tgaaaccgcc
    20941 gccctgaatc ataaagccag gaatgacgcg gtgaaaggta gtgttgttat aaaaaccgct
    21001 gttcacataa tcgacaaagt tttgcacaga cactggcgct ttttgtttat ccagctccag
    21061 ttcgatgtta ccagctgagg ttgtcaacaa tacgtgcggg tcccctttcg ctgccattgc
    21121 tgcgggagaa agagcagaaa gagcgaaaac agcagccatc gccgccaggg tcgatttgaa
    21181 catgagattt ccttaacaaa cagagcagaa aaaaagcaag tgaagagatt ctaaatagcc
    21241 ttcaagaaca aggccatccc tttacctaat tttacgtatc tgaaatatat gtaacctgac
    21301 gatcactttt tattccgtga tcaaaatcac ctcttaaaat gcaatttagc aaccgattgc
    21361 aataaaacat ttaaacagat cacaaaatca cctaaaatcg cccgctcgcg gcatatcccg
    21421 ctatgccatt ttatttttga acgcaggcca atcatgacta acagcaatcg catcaagctc
    21481 acatggatta gctttctctc ctacgcactg actggtgcgt tggttattgt caccgggatg
    21541 gtgatgggaa atatcgccga ttatttcaat ctgcctgttt ccagtatgag taacaccttc
    21601 accttcctca acgccggcat tttaatctct atcttcctca acgcctggct gatggaaatc
    21661 gtcccgttga aaacgcagtt acgttttggc tttctcctga tggtgctggc ggttgccggt
    21721 ttgatgttca gccacagcct ggcgctgttc tcgacggcga tgttcattct cggggtggtc
    21781 agcggcatta ctatgtcgat tggtacattc ctgataacac aaatgtatga agggcgtcag
    21841 cgcggttccc gcctgttatt tactgactcc ttcttcagta tggctgggat gattttccca
    21901 atgatcgccg cgtttctact ggcgcgcagc attgagtggt actgggttta tgcctgcatc
    21961 gggctggtgt atgtcgctat ttttattctg accttcggct gtgagttccc ggcactgggc
    22021 aaacatgcac caaaaacgga tgctccggta gagaaagaaa agtgggggat cggcgtactg
    22081 tttctctctg ttgcggcgct gtgctacatc ctcggtcagt taggttttat ctcctgggtg
    22141 cctgagtatg ccaaaggcct gggcatgagc ctgaacgacg cgggcacgct ggtgagtaac
    22201 ttctggatgt catacatggt cggcatgtgg gcgttcagct ttattcttcg cttctttgat
    22261 ttgcaacgca ttctgaccgt actggctggt ctggctgcga ttctgatgta cgtctttaac
    22321 accggaacac cagcacatat ggcgtggtca attctcgctc tgggcttctt ctccagcgcg
    22381 atctatacca ccatcatcac cctgggttca cagcagacca aagtaccgtc gccaaaactg
    22441 gttaactttg tcctgacctg cggaaccatc ggtactatgt tgacctttgt ggttaccggc
    22501 ccgattgttg aacatagcgg tccgcaggcg gcactgctta cagcaaacgg tctgtacgct
    22561 gtcgtctttg tgatgtgctt cctgttaggt ttcgtcagcc gtcaccgtca gcataacacc
    22621 ctgacctctc attaattgct aatgccggac ggcactatcg tcgtccggcc ttttcctctc
    22681 ttcccccgct acgtgcatct atttctataa acccgttcat tttgtccatt ttttgcacaa
    22741 acatgaaatg tcagacaatt ccgtgactta agaaaattta tacaaatcag caacatacca
    22801 cttaaggagt atataaaggt gaatttgatt tacatcaata agcggggttg ctgaatcgtt
    22861 aaggtaggcg gtaatagaaa agaaatcgag gcaaaaatga gcaaagtcag actcgcaatt
    22921 atcggtaacg gtatggtcgg ccatcgcttt atcgaagatc ttcttgataa atctgatgcg
    22981 gccaactttg atattaccgt tttctgtgaa gaaccgcgca tcgcttatga ccgcgtacac
    23041 ctctcgtctt acttctctca ccacaccgcc gaagagctgt cgctggtgcg cgaaggcttc
    23101 tacgagaaac acggcatcaa agttctggtc ggcgaacgcg ctatcaccat caaccgtcag
    23161 gagaaggtga ttcactccag cgccggacgt accgtttttt atgacaagct gatcatggca
    23221 accggttcct acccgtggat cccaccaatc aaaggttctg atactcagga ctgctttgtc
    23281 tatcgcacca ttgaagacct caacgccatt gaatcctgcg cccgtcgcag caaacgcggt
    23341 gccgttgttg gtggcggcct gttaggtctg gaagccgctg gcgcgctgaa aaacttaggt
    23401 attgaaaccc acgttatcga atttgcccct atgctgatgg cggaacagct tgatcagatg
    23461 ggtggcgagc agctgcgtcg caaaatcgaa agtatgggcg tgcgcgttca caccagcaaa
    23521 aacacccttg agattgtgca ggaaggtgtc gaagcgcgta aaaccatgcg ttttgccgac
    23581 ggcagcgaac tggaagtcga ctttatcgtc ttctctaccg gtatccgtcc gcgcgataag
    23641 ctggcaaccc agtgtggtct ggacgttgct ccgcgtgggg gtatcgtcat taatgattcc
    23701 tgccagactt ccgatccgga tatctacgcc atcggtgaat gcgcaagctg gaacaaccgt
    23761 gtatttggtc tggtagcacc tggctacaaa atggcgcagg tcgccgttga ccatattctc
    23821 ggtagcgaaa acgcctttga aggtgctgac cttagcgcca agctgaaact gctgggcgta
    23881 gacgtaggcg gtattggtga tgcgcacggt cgcacgcctg gcgcacgtag ctacgtttac
    23941 ctcgacgaaa gtaaagagat ctacaaacgc ctgattgtca gcgaagacaa caaaaccctg
    24001 ctcggtgcgg tactggtggg cgataccagc gactacggta acctgctgca actggtgctg
    24061 aacgctatcg aactgccgga aaacccggat tccctgatcc tgccagcaca ctctggtagc
    24121 ggcaagccgt ctatcggtgt tgataaactg ccggacagcg cgcaaatctg ctcctgcttc
    24181 gacgtcacta aaggtgatct gattgctgcc atcaacaaag gctgccacac agttgcggcg
    24241 ctgaaagctg aaaccaaagc gggtactggc tgcggtggct gtatcccgct ggtcactcag
    24301 gtactgaacg cggaactggc gaaacagggc atcgaagtta acaacaacct gtgcgaacac
    24361 tttgcttatt cgcgtcagga actgttccat ttgatccgcg ttgaaggcat taaaaccttc
    24421 gaagaactgc tggcgaaaca cggcaaaggc tacggttgtg aagtttgtaa accaaccgtc
    24481 ggttcgctgc tggcctcctg ctggaacgaa tacattctga agccggaaca tactccgctg
    24541 caggattcta acgacaactt cctcgctaac atccagaaag acggcaccta ctcggtgatc
    24601 ccgcgttctc cgggcggtga aatcaccccg gaagggctga tggcagtggg tcgtatcgcg
    24661 cgtgaattta atctctacac caaaatcacc ggctcccagc gcctggcgat gtttggcgca
    24721 cagaaagacg atctgccgga aatctggcgt cagctgattg aagctggctt cgaaaccggt
    24781 catgcctatg cgaaagcact gcgtatggcg aaaacctgcg tgggtagcac ctggtgccgc
    24841 tacggtgttg gcgatagcgt cggcctcggc gtggaactgg aaaaccgcta caaaggcatc
    24901 cgtacgccgc acaaaatgaa gttcggtgtc tccggctgta cccgtgaatg ttcagaagct
    24961 cagggtaaag acgtgggtat tatcgccact gaaaaaggct ggaacctgta tgtttgcggt
    25021 aacggcggca tgaaaccgcg tcatgcggat ctgctggcag cggatatcga tcgcgaaacg
    25081 ctgatcaaat atctcgaccg cttcatgatg ttctacatcc gtaccgccga caaactgacg
    25141 cgtaccgcgc cgtggttaga aaacctcgaa ggcggcatcg attacctgaa agcggtgatc
    25201 attgacgaca aactggggct gaacgctcat ctggaagaag agatggcgcg cctgcgtgaa
    25261 gcggtagtgt gtgagtggac tgaaacggtc aatacgccgt ctgcgcagac tcgcttcaaa
    25321 cacttcatca acagcgacaa gcgtgacccg aacgtgcaga tggtgccaga gcgcgaacag
    25381 caccgtccgg caacgccgta tgaacgtatc ccggtaactc tggtggagga caacgcatga
    25441 gccagtggaa agacatctgc aaaatcgatg acatcctgcc tgaaaccggc gtctgcgcgc
    25501 tgttaggtga cgagcaggta gcgattttcc gcccgtatca cagcgaccag gtgtttgcga
    25561 tcagcaacat tgatccgttc ttcgagtcca gcgtgctatc acgcgggctg attgcggaac
    25621 accagggcga gctgtgggtc gccagcccgc tgaaaaaaca gcgttttcgc ttaagtgatg
    25681 gcttgtgcat ggaagacgaa cagttttccg tcaaacatta cgaagcgcga gtgaaagacg
    25741 gcgtggtgca gctgcgcggt taatgtttta acgggaggcg caatgcctcc cctttttgca
    25801 tggtc
//
LOCUS       DAEEKQ010000046        25648 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.046, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000046 DAEEKQ010000000
VERSION     DAEEKQ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25648)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25648)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25648
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.046"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(76..807)
                     /gene="rlmB"
                     /locus_tag="KLK57_001817"
     CDS             complement(76..807)
                     /gene="rlmB"
                     /locus_tag="KLK57_001817"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HBE0892201.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(987..3428)
                     /gene="rnr"
                     /locus_tag="KLK57_001818"
     CDS             complement(987..3428)
                     /gene="rnr"
                     /locus_tag="KLK57_001818"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HBE0892202.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYVPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="KLK57_001819"
     CDS             complement(3467..3892)
                     /gene="nsrR"
                     /locus_tag="KLK57_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HBE0892203.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4097..5395)
                     /gene="purA"
                     /locus_tag="KLK57_001820"
     CDS             complement(4097..5395)
                     /gene="purA"
                     /locus_tag="KLK57_001820"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HBE0892204.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVATTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5499..5696)
                     /locus_tag="KLK57_001821"
     CDS             complement(5499..5696)
                     /locus_tag="KLK57_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HBE0892205.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="KLK57_001822"
     CDS             complement(5778..6782)
                     /gene="hflC"
                     /locus_tag="KLK57_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HBE0892206.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="KLK57_001823"
     CDS             complement(6785..8044)
                     /gene="hflK"
                     /locus_tag="KLK57_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HBE0892207.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="KLK57_001824"
     CDS             complement(8130..9410)
                     /gene="hflX"
                     /locus_tag="KLK57_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HBE0892208.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="KLK57_001825"
     CDS             complement(9487..9795)
                     /gene="hfq"
                     /locus_tag="KLK57_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HBE0892209.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSCAQNTSAQQDSEETE
                     "
     gene            complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="KLK57_001826"
     CDS             complement(9881..10831)
                     /gene="miaA"
                     /locus_tag="KLK57_001826"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HBE0892210.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="KLK57_001827"
     CDS             complement(10824..12671)
                     /gene="mutL"
                     /locus_tag="KLK57_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HBE0892211.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKLKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLALPVAERWLRQVQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="KLK57_001828"
     CDS             complement(12681..14018)
                     /gene="amiB"
                     /locus_tag="KLK57_001828"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HBE0892212.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="KLK57_001829"
     CDS             complement(14037..14498)
                     /gene="tsaE"
                     /locus_tag="KLK57_001829"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HBE0892213.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="KLK57_001830"
     CDS             complement(14470..16017)
                     /gene="nnr"
                     /locus_tag="KLK57_001830"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HBE0892214.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            16016..17155
                     /gene="queG"
                     /locus_tag="KLK57_001831"
     CDS             16016..17155
                     /gene="queG"
                     /locus_tag="KLK57_001831"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HBE0892215.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCIACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAQ
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILAALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17138..17191)
                     /locus_tag="KLK57_001832"
     CDS             complement(17138..17191)
                     /locus_tag="KLK57_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892216.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17425..17500)
                     /locus_tag="KLK57_001833"
     tRNA            complement(17425..17500)
                     /locus_tag="KLK57_001833"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17465..17467),aa:Gly,seq:gcc)
     gene            complement(17536..17611)
                     /locus_tag="KLK57_001834"
     tRNA            complement(17536..17611)
                     /locus_tag="KLK57_001834"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17576..17578),aa:Gly,seq:gcc)
     gene            complement(17769..17844)
                     /locus_tag="KLK57_001835"
     tRNA            complement(17769..17844)
                     /locus_tag="KLK57_001835"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17809..17811),aa:Gly,seq:gcc)
     gene            complement(18055..18600)
                     /gene="orn"
                     /locus_tag="KLK57_001836"
     CDS             complement(18055..18600)
                     /gene="orn"
                     /locus_tag="KLK57_001836"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HBE0892217.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18695..19747
                     /gene="rsgA"
                     /locus_tag="KLK57_001837"
     CDS             18695..19747
                     /gene="rsgA"
                     /locus_tag="KLK57_001837"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HBE0892218.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19844..20812
                     /gene="psd"
                     /locus_tag="KLK57_001838"
     CDS             19844..20812
                     /gene="psd"
                     /locus_tag="KLK57_001838"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HBE0892219.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKICQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20834..24157
                     /gene="mscM"
                     /locus_tag="KLK57_001839"
     CDS             20834..24157
                     /gene="mscM"
                     /locus_tag="KLK57_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HBE0892220.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNMRDKPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(24308..>25648)
                     /gene="yjeM"
                     /locus_tag="KLK57_001840"
     CDS             complement(24308..>25648)
                     /gene="yjeM"
                     /locus_tag="KLK57_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HBE0892221.1"
                     /translation="LMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIW
                     MVSTSAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKI
                     ARITAVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVV
                     FAIFAYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVL
                     SNGSVNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFT
                     LCYSPLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASA
                     FFNKLTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTRMSAMIAMVVVVLVVT
                     FANVFTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRMANKPELALD"
BASE COUNT         5935 a         7193 c         6500 g         6020 t
ORIGIN      
        1 tcaccgtaat tatcttacca gctatagaca aaaaaaacca tccaaatctg gatggctttt
       61 cataattcag acgaactagc tgcgctgacg aaccgcttca aataagcaaa ttccggttgc
      121 aaccgaaacg ttcagggaag aaacacttcc tgccattggg atgctgatca actcatcgca
      181 atgttcacgg gtcaggcgac gcataccttc accttccgcg cccatcacca gcgccaagcg
      241 tccggtcatt ttgctctgat agagtgtatg atccgcctcg cctgccgtac cgacgatcca
      301 gatattctct tcctgcaaca tacgcatggt gcgcgcaagg ttagtcaccc gaatcagtgg
      361 aacgctttct gccgcgccgc aggctacttt tttcgccgta gcgttgagct gtgcggagcg
      421 atctttcggc acaatcaccg cgtgaacgcc agcagcgtcc gcgctacgca ggcacgcgcc
      481 gaggttgtgc ggatcggtta caccgtcgag gatcagcagg aacggttgat cgagcgaagc
      541 gatcagatcc ggcagatcgt tttcctgata ctgacgtcct ggcttcacgc gggcgataat
      601 gccctgatgc acggcaccgt cgcttttctc gtcgagatat tggcggtttg ccaactggat
      661 aaccacgccc tgagactcaa gggcgtgaat cagcggtaac agacgtttat cttcacggcc
      721 ttttaaaata aagacttcct gaaaacgttc aggggcgcgc tccagcaggg cctgcactgc
      781 gtggatgccg taaatcattt cgctcattaa tgtactcgtt gtttacgttt tgcctgatgc
      841 actacgttta tccggcctac atgatctctg caatatattg aatttgcgtg attatgtagg
      901 ccggataagg cgttcacgcc gcatccggca tgaacaaagc gcattttgtc agcaatctaa
      961 ccctcttctt ttaaagaggg tattgatcac tctgccactt ttttcttcgc cgcacgcttc
     1021 gctttggtcg ctgcggctat tttctgcgtt ttcgccgatg gctttttcgc ttttctcgcg
     1081 tctttcttcg ccgctttcgg cttcgttttt ttctcaccgc ggaaggcgct gtctggctca
     1141 aagtttacct ttttaccgac ctgacgacgc ttgccgcctt ttttgcctgc atcgcctttt
     1201 ttcgctttct cgcgcgccgt tttaccgacg ttgcgcggtg cgcgttcact ggagatcaga
     1261 ctaaagtcga ttttgcgctc gtccatatta accgcttcga cgcgaacttc cacgcgatcg
     1321 cccaggcgat aagtctggcc gctggattcc cccatcaggc gttgccctac ctggtcaaag
     1381 cgatagtagt cattgtccag cgaagagaca tggaccagac catcaatgaa caagtcgtcc
     1441 agacggacga agaagccaaa gccagtgacg ctggaaatta cgcctttaaa gacgttacct
     1501 acctggtcga gcatgaagtc acacttcagc cagtcagcga catcgcgcgt tgcttcgtcg
     1561 gcacgacgtt ccgccatcga acagtgctga cccagttgca acatctcttc catcgaataa
     1621 tggtagccgc cggtttcagt ggtgttgccc tgatgcccct gctctttcgc cagcagatac
     1681 ttaatggcgc ggtgcagcgt caggtcagga taacgacgaa tcggcgaagt aaagtgcgca
     1741 taggactgca atgccaggcc aaagtgacca cggttttctg gatcgtaaat cgcctgtttc
     1801 atcgagcgca gcagcatggt ttgcagcatt tctgcatcag gacggtcggc aaccgactcc
     1861 agcagttccg cgtagtcacg cggttccggc ttgttaccac ccggcagctc cagccccagc
     1921 tccgccagca ctgaacggaa agaggtaatc gcttcggtgc tcggcttgtc gtgaatacgg
     1981 aacagtgccg gttctttcgc tttctcaacg aaacgcgccg ccgagatatt cgccagaatc
     2041 atgcactctt caattaattt gtgcgcgtcg ttacgctggg tctgttcgat acgttcaata
     2101 cggcgttcag cgttgaaaat gaacttcgct tcttcgctct caaatgagat cccaccgcgt
     2161 tcttcacggg ctttatccag cactttatag aggttatgca actcttcgag atgcttaacc
     2221 agcgggacgt actgctcgcg cagatcctga tcgccctgca gaatatgcca gactttggtg
     2281 taggtcagac gcgcgtgaga gctcatcacc gcttcgtaga atttgtagcc cgtcaggcgg
     2341 cctttcgacg aaaccgtcat ctcgcacacc atacacaggc ggtctacctg cgggttgagc
     2401 gaacacaggc cgttagagag cacttccggc agcatcggga taacctgcga agggaagtac
     2461 accgacgtgc cacggttacg cgcttctctg tccagcggcg ttggcggacg cacatagtag
     2521 ctgacgtcgg caatcgcgac ccataaacgc cagccgccgc cgcgtttttt ctcgcagtaa
     2581 actgcatcgt caaagtcacg ggcgtcttcg ccatcaatgg tgaccagcgg taaatcgcgc
     2641 aaatcgacac ggcccgcttt tgcttcttcc ggcacttctt ctttcagccc cgcaacctgt
     2701 tgctcaacag cctgcggcca gatatacgga atttcatggg tacgcagagc gatatcaacc
     2761 gccatgccgg tgcccatatt gtcgcccagc acttcgacga ttttacccac cgctttggtg
     2821 cggcgagtcg gacgctgagt cagttcgact acgaccacaa agcccatccg cgcgcccatg
     2881 atctgatcgg gcgggattaa gatatcgaag ctcagacggc tatcgtcagg aaccacaaag
     2941 ccgacgcccg cttcggtaaa gtagcgacca acaatctggc tggtttttgg caccagtacg
     3001 cggacaatac gcgcttcacg acgaccttta cggtcagcac ccagcggctg cgccagcacc
     3061 tgatcgccat gaatacaggt tttcatctgc tcgctggaga gatacaaatc atctttacgc
     3121 ccttcaaccc gcagaaagcc gtagccatca cggtggccaa taacggtacc tttcaccagg
     3181 tcgaggcgtt ccggcagcgc atagcactga cgacgagtga agaccagttg accatcgcgc
     3241 tccatcgcgc gcaggcggcg acgcaggcct tcaagctgct cttcgccttc aatgtgcagt
     3301 tctaccgcca gctcatcacg gctggccggt ttttcacgtt tggttaaatg ttcgaggata
     3361 aattcccgac tagggatggg attcgcgtat ttttcagctt cgcgttcctg gaaaggatct
     3421 tgtgacatct cggttcctcc gttgtcatct ctgatgaaga ttttcgtcac tccaccagca
     3481 ataatttata aagcggttga ttctcttcaa ccaaatcggc aagcgtgtag ttatccagtt
     3541 ccgtaagaaa actttgcacg gccttagaaa gcgcctgttt caaccgacag gcaggtgtaa
     3601 tgtggcaaaa ctcactgctg caattcacca gcgataaggg ctccagctcg cgcaccacat
     3661 caccaatacg tatcgcactc gccggtttac ccaggcgaat gccgccattt tttccacgaa
     3721 cagcagtcac gtagccggca cgactaagtt gattgattat tttgaccata tgattacggg
     3781 agacgccgta gacgtcagtc acttcagaaa tactggtcat ccgcccttct ggcaatgacg
     3841 ccatgtagat cagcgcacgt aatccgtaat cagtgaaact cgttaactgc acatcaacct
     3901 caaaagggaa atcgggaaaa aatacattta tattgatgat aaaccagcca caagctgtgt
     3961 cgctaattta tttcagttcg gggaaggaaa aaagcgagga tttaacactg tgtcggatag
     4021 cgtaatggct taaccgacac agcaatgtat cacactgtta gcccggcagg caaaatatct
     4081 gccaggcgta ccagaattac gcgtcgaacg gatcgcgcag aatcatggtt tcagtacgat
     4141 ccggaccggt agagatgata tcgatcggca caccggtcag ctcttcaata cgcttgatgt
     4201 agttcagcgc cgcctgcggc aggccgctac gatctttcac gccgaaagtg gattcagacc
     4261 agcccggcat ggtttcgtaa atcggctcta cacctttcca gtcgtcagct gccagcggag
     4321 tggtagccac ttcgcgacca tccggcatac ggtaagccac gcagagttta acctctttca
     4381 ggccatccag aacgtccagc ttggtcaggc aaaagccaga cagggagttc agctgtaccg
     4441 cacgacgaac ggcaacggtg tccagccagc cggtacgacg acgacgaccc gtagttgcgc
     4501 cgaattcgtt accctgcttg cagaggaact cgccagtttc atcaaacagt tcggtcggga
     4561 acggacctgc acctacacga gttgagtaag ctttgaggat acccagaaca taatcaacat
     4621 aacgcgggcc caggccggaa ccggtcgcca cgccaccagc agtggtgttg gaagaagtta
     4681 cgtacggata agtaccgtgg tcgatatcca gcagcgtacc ctgcgcacct tcaaacatga
     4741 cgaaatcgcc acgctgacgc gcctggtcga gcaggtcaga aacgtcaacc accatagaag
     4801 tcaggatgtc ggcaacagcc atcgtatcat ccagaacttt ctggtaatca accgcttcag
     4861 ctttgtagta gttaaccaac tggaagttgt gatattccat cacttctttc agtttttcag
     4921 cgaaggtttc tttgtcgaaa aggtcgccaa cacgcagacc gcgacgtgct actttatctt
     4981 cataagcagg cccgatacca cgaccggtgg tgccgatcgc tttcgcgcca cgcgctttct
     5041 cacgcgcgtt atccagcgca acgtgataat caaggatcag cggacatgct tcagacagca
     5101 gcagacgctc acgaacgggg atgccacggt cttccagttc tttcatctct ttcatcagcg
     5161 cggccggaga cagcacaaca ccgttaccga tgatgctggt tacattctcg cggagaatac
     5221 ctgatggaat aagatggaga acggtttttt caccgttgat tacgagagta tggcctgcgt
     5281 tgtgaccgcc ctggtagcgt acaacatatt tagcccgttc agtcagaaga tcgacgatct
     5341 tacctttacc ttcgtcaccc cattgggtgc ccagtacgac gacgttgtta cccatttttc
     5401 aaaatcaccg tttgcttaaa aatggattct accatcgctt tttcagagtt acagcacttt
     5461 ttgcactcaa aaatgacctc gatcagtctg ttttttgttc agccaatcgt tttcctcaac
     5521 atgtagtaga ccacaacgcc cgcaaccaca agtccaccgc caaaacgacg taaaatatta
     5581 tcgggcaaat tggtcatcgc agagatcatc ttcttccatg ccttcgggta aagcatcggc
     5641 cctaaacctt ccagtaccaa aaccagggca agcgccagcc agattgtcga attcattcat
     5701 tatccttata gaaaaagaaa accaccgaca tccctgagga tgcggtggct ttattgacct
     5761 gtaccgcagt cgttatatta acgcgttgcg gaagtcggcg tcttcatgta gcggaagaaa
     5821 tcgctatccg ggctcatgac catcacgtcc tgattgccag agaagctgtt ctcataagca
     5881 cgcaggctac ggatgaatgc gtagaagtcc ggatctttac tgaatgcatc agcaaacagt
     5941 ttggctgctt cggcatcgcc ttcaccacgc atgatgcggc cctgacgctc agcttctgcc
     6001 agcgttctgg tcacttcata gtcggcagtc gcgcgcagtt tttccgcttc ttcctgacct
     6061 tgtgaacggt gacgacgcgc taccgcttca cgctcggcgc gcatacggtt gtagatcgct
     6121 tcagacactt cggtcggcag gttgatctgc ttgatacgca catcgacaac ttcaataccc
     6181 agcgccgcca tactgttcgg gttgatgacc ggaactttgc ccttcgtctc agccgttacg
     6241 cgctctgccg cttcggcaat ggcgttatct gccgccgggg tagtaacttc atcttctgta
     6301 cccgcagaac cggagttcag cgcgtcacgt acttcgaggg tcagacgacc acgggaatcg
     6361 gtgacgatgt ctttcacgtc caggcgacca atttcagaac gcagacggtc agagaactta
     6421 cgtttcaaca gcacttccgc ttgcgaaatg tcgccaccac ccgttgccag gtagtaacgg
     6481 ctgaaatcgc tgatgcgcca tttgatgtaa gaatcgacga tcaggtcttt cttctctttg
     6541 gtcacaaagc ggtcggcctg gttgtccatg gtctgaatac gtgcgtcgag cattttcacc
     6601 gtttcaatga acggtatctt gaaatgcaga cccggctcat aaaccagagg tttgttgtca
     6661 tcgtcacgca gtaccttacc aaaacgcagc gtaataccgc gctcaccttc tttgacgaca
     6721 aagacagaca tgtaaagcac taccagcacg atgatgataa tcgcgataac tgacttacgc
     6781 atcgttattc cccctgacgc tggtagtcgt tacgctgcgc gttggcgcgg cgttggtcca
     6841 taatatcgcc ctgactggtg gacgacgtgt tgcttgctcc actggttgtg gaggaagagg
     6901 ctggcggcag acgcagcaga ttgctggcac cgttatcgct cttcgccgca ggggcgttac
     6961 cgcctttcag catctggtct aacggcagaa ccatcaggtt gccaccttta tcgttaacca
     7021 gcactttgcg ggtgttaccc aacacttttt ccatcgtctc gatatacaga cgctcgcgag
     7081 taatttccgg cgcggcttta tattccggca gaagtttagc aaagcgcgcc acttcaccct
     7141 gagcttccag gatggtctgg gccttgtacg cacgcgcctc ttcgaggata cgttgcgcct
     7201 gaccgttcgc acgcggctga acttcgttgg tatacgcttc tgcttcacga atgtattgct
     7261 gttcgttttc acgcgcggca atcgcatcgt caaacgccgc ttttacttct tccggcggac
     7321 gagctgcctg gaagttgacg tccagcagag tgatacccat gtcatacgga cggatagtct
     7381 cttccagttc gcgctgagta tcgctacgaa tcacggtacg accttccgtc agaatgcggt
     7441 ccatggtgta tttaccgata actccacgca gggcgctgtc ggtagcctga cgcaggctgt
     7501 catccgggct ggtcacgcta tacagatatt tttccggatt ggtgacgcgg tactgcacgt
     7561 tcatctcaac gcgcactacg ttctcgtccg acgtcagcat cacaccagaa gcggccagtt
     7621 cacgcacggc ttccacgttc accggtttga cttcgtcgat aaacgtcggt ttccagttca
     7681 gacccggctc aaccagatgg ctgaatttac caaagcgtgt taccacgccg cgttcggctt
     7741 ctttaatggt atagaaacca ctggccgccc agataatgac aatcgctgcc gctgcgatgg
     7801 taacgacacg accgccaagc tgcgggcgcg ggccttgcga tgaactgcca ccgccagatc
     7861 cggtgccttt accgccgccc agaccaccga gctttttgct cagtttgcgg aagatatcat
     7921 ctaaatcagg tggcccttga tcgcgaccgc ctttgtttcc atttccctca gagttgccgc
     7981 caggtttgct gcttccccac gggtcgcggt cttgtccgtt attaccgggc tgattccacg
     8041 ccatgtttgt gctccatatt tgttatgcgg tgatccctgt tgtctttcga cgccagagga
     8101 tatgactcca cgcttcagac gctacgccgt tagatcaggt aatcgatcaa cgccggttct
     8161 tgtttacaga ggcgacgcca gtcaacgatc ggcatacgaa cttgcagact tacgctgccg
     8221 tcctcctcca tccactcttt ttctattgcc tgaagctgat aaaaacgact tctcagacgt
     8281 ccttcctgcg gcggcaaacg caatgtatgc tgcgccacct cgccggaaag ccgctccgtc
     8341 aaagcctgaa aaagctgtgg tatccccgct ccggtctgtg cggaaagcca gacacggatc
     8401 ggtttgttct cttcgtcccg atcaatacgc ggttcgaaat cttccagcat atcgatcttg
     8461 ttcatcacca gcagggttgg gatctcgtga gcgtcgatct cttcaagaac cgtattcacc
     8521 gcttcgatgt tttcttgtac acgtacatcc gccgcatcaa tgacgtgcag cagtaatgtg
     8581 gcttgccgcg tctcttgtaa cgtggcttta aatgctgcca ccagatcgtg cggcaggtgg
     8641 cgaataaacc ctacggtatc tgcaagtacg gtttcaccga catccgcgac gtcaatacgc
     8701 cgcaacgtcg ggtcgagggt ggcaaacaac tggtctgccg cgtagacccg cgcttcggtg
     8761 atgcgattga aaagggtaga tttaccggcg ttggtatatc ccaccagcga aacagtagga
     8821 acgtcggctt tgatacgcga ttgccgcccc tgctcacgct gcttttcaac tctttctagg
     8881 cgcgactgta tctgcacgat gcgattacgc aacaaacgac ggtcggtttc gagctgggtt
     8941 tcacccggac cacgcaaacc tatcccgcct ttctgtcttt caaggtgggt ccagccacgc
     9001 accaggcgcg tagccagatg gcgcagctgc gctagctcaa cctgcaactt accctcatgg
     9061 gtacgcgcac gttgggcgaa aatatctaaa ataaggccgg tgcggtcgat aacacgacac
     9121 tcgcacaaac gctccaggtt acgctcttgc gccgggctca gggcatggtc aaaaagaacg
     9181 accgaagcac ccgtcgcttt gacagcttcc gcaatttcaa ctgctttacc ttcacctaca
     9241 aaatactttg ggtgcggcgc tttacggcta ccggtaatca cctgcaatgc ttcgacaccg
     9301 gcggaagaga ccagagattc aaactcctgg aggtcttcca tatctttgtc ttgcgtaaaa
     9361 tagatgtgta ccagtaccgc ctgctcacca gcatcataac ggtcaaacaa gcgtataacc
     9421 ctctaaatag atcagcgggg aacgcaggat cgctggctcc ccgtgtaaaa aaaacagccc
     9481 gaaaccttat tcggtttctt cgctgtcctg ttgcgcggaa gtattctgcg cgcagctacc
     9541 atgatggtag ttactgctgg taccgccacc ggcgttgtta ctgtgatgag aaaccgggcg
     9601 agacgggaca acagtagaaa tcgcgtgctt gtaaaccatc tggctgaccg tgtttttcaa
     9661 caggatcacg aactgatcaa aagactcgat ttgcccttgc agcttaatac cattcaccaa
     9721 ataaatagaa actggaacac gttcccgacg cagtgcgttc aggaacggat cttgtaaaga
     9781 ttgcccctta gccattctct cttttcctta tatgcttatt tgtactttga acctttcgat
     9841 tctgaaaaaa ttgcgcacga tacgtctcaa ttgtacacat tcagcctgcg atagcaccaa
     9901 caacctgtaa tacttcgtca cgcgcctgtt ctggtttttc actgtcaagc cagtgaaccc
     9961 cttcccaacc acgcagccag gttatctgcc gcttcgccaa ctgtctcgtg gcgcaaacac
    10021 ctcgataaac catttcatcg tatgagattt cgccttcaag gtaagaccac atctggcgat
    10081 aacccacgca acgaatggaa ggcaagtccg tatgcaaatc tcctcgggca aaaagcgccc
    10141 ggacttctgc ttcaaaacct gaagccaaca tctgatgaaa acgctgctca atgcgttgat
    10201 ggagcagttc acggctcgcc ggggcgatgg cgaactgatg cacctgatac ggtagagcgt
    10261 ctcctgacgt ttgcgtcagt tccgttaaag ttttacccga aatgaaaaaa acttccagtg
    10321 cccgggaaag cctttgtgga tcatttggat gaatccttgc tgccgcaacc ggatctacct
    10381 cctgaagttg acgatgcaat gactcccaac cttgctctgc cgcctgttgc tcaattctgg
    10441 cccgcacttc cgggtctgcc gacggtagcg gcgacaaccc ttccagcaat gccttgaaat
    10501 acaacatcgt accgcccact aacagtggga tccgccccgc cgcggtgata tcggccattt
    10561 ccgccagcgc atcgcggcga aaatcagcag ccgagtaagc ctgcgacgga tcgcgaatat
    10621 ccagcaatcg gtgcggcgcg gcgagtaact cttcagcgtt cggcttcgcc gtcccgatat
    10681 ccatcccttt gtaaataagg gcagaatcaa cgcttatcaa ctctactggt aaaattttac
    10741 gcagctcaat ggctaacgcc gttttaccgg aggccgtcgg ccccatcaaa aaaatcgcct
    10801 taggcaggct cgccttactg atatcactca tctttcaggg cttttatcgc cggatgtaaa
    10861 tcaacagatt gtaacagacc acccggcggc gttttcacaa gttgcggaca taaccgttcc
    10921 acgtccgcca gcagggttat ggcctgtgcc attgaccact gcgcatgttc gctcatcaga
    10981 tttcgtgcaa tccactgcgc aatattgcca ggttcgaata cggactgctt cgccaggtag
    11041 cctatcagtt caggaatcaa gatttgtaaa ttttgttggc gtaagggtaa aggcacggcc
    11101 ctgatggtca catgctgtgc atctgactgg aaatcaatac ccaattccgc cagggcagac
    11161 tgcgcttttt ctaatgccga tttttcttcg ccagaaactt ttagccgcaa cggaataagc
    11221 agcggctgag cgcaaactgg cgcttcaccc ggcgtcagtt gtacctgacg cagccaacgt
    11281 tctgccactg gcaaggctaa aagtgaaatg ttgccgtcgc gctccagcaa cgcacagtcg
    11341 gaatggacga tagtcagtac ccgaccaaaa ctctgactgt tcgccgcaag tgcaggttcc
    11401 tgcggttccg gcgcttttaa tttttgcatc ggcgcgggcg tttgcaaaag ctggcgatac
    11461 acttcacctt gctgtttctg gtagcctggc tgcgcattcg gccagggggc agccggacga
    11521 ctgcctgatg ctggcgcagg agtgtagcgc ggagctaccg gctcacgagc tgccggttct
    11581 gcaaagtgat tgcgccccgc cgccacgcgg ttttccggaa tggaacgcgg tgcaggttgg
    11641 ggttcatcgt ccagcggtag cggcgtttcc agttgctgtt gtagcacgct cagcacgccc
    11701 tgatagataa aatcatgcac cagacgcgac tgatggaaac gcacttcgtg tttggcgggg
    11761 tgcacgttga cgtccacctg atgcggatcg atctccagat acagcacaaa tgccggttgc
    11821 tgatcggccc ccagtttgtc ttcgcaggcc tggcggatcg cgtgattgat caggcgatcg
    11881 cgcatcatgc gaccgtttac gtagcaatac tgaatttctg ccagtgcggg cgtggtgtga
    11941 tttggatcgg ccacccagcc gcgtagcgtg agatcgccgt gttgccattc aatcgccagc
    12001 gcttgttcaa gaaaagcggt gccgcaaatc gcgcctaagc gccgttcttt ttgcccgcct
    12061 tccggcactg cgcggtactg acgcacaatt ttaccgttat gcgacaggtt gatcgtgacg
    12121 tcgaaacgcg ccagcgcaat gcggcggatg atctcatcaa tgtggttaaa ttcggttttc
    12181 tcggtgcgca ggaatttgcg ccgcgccggg gtgttgtaga acagatccag cacctccagc
    12241 gtcgtcccca caggatgcgc cgccggtttt accgtcacgt tcatatcgcg cccttcggca
    12301 taggcctgcc aggcttcctg ctgttctgcg gtgcgtgaag tgagcgtcag gcgggaaacc
    12361 gaactgatac tcgccagcgc ctcaccgcga aagcccaggc tgataatggc ttcgagatcg
    12421 tccagagagg cgattttact ggtggcatga cgagccagcg ccagcgccag ctcatctttt
    12481 ttgataccgc agccgttatc acgaatgcgg ataagtttcg ccccaccgcg ttcgatatca
    12541 atatcgatac gcgtcgcacc tgcatcgagg ctgttttcca ccagttcttt gactaccgac
    12601 gcaggtcgct cgaccacctc acctgcggca atctggttcg ccagttgtgg cggtaagacc
    12661 tgaattggca tcaatcgtcc ttagtttggc agcgtgcgat ctggcgtcgt caccgtactg
    12721 gcagtttgtg ccgttgcccc ctgcggcgca gattgcatcg gatgcgcaag gaaataattg
    12781 cgcaggcctt tgtaaatggc ttctgccagc tgttgttggt aatcgtcgct cgccagcaaa
    12841 cgttcttcgc tgttgttgct gataaaaccg gtttcgacca gtactgacgg gatatccggc
    12901 gaacgcagaa cgccgagact cgcgtgttct ggtcggcgtt tatgtatttc gccaatgcgt
    12961 tgcaactgac tgatcatact ggtcgctaca tcatacccta cccgctggga atgaccgaac
    13021 tgtaaatcca gcaccgcctg gcttaaatag gggtcagact gactgttcgc cagcacatcg
    13081 cccgccccac ccagcagctc cgactgtttc tcgtgctgct ccagccagct ggccatttcg
    13141 ctgttggcgc gacggttaga gagcacccat acggaagcgc cagtcgcact gcggttcggt
    13201 gcggcatcag cgtgaatcga cacgaggaaa ttggcgtttt gcttacgtgc cacatcgctg
    13261 cgccccatca ccgagataaa gtaatccccg tcacgggtta aaacgccttt aaacatcgga
    13321 tcgtcattga gcaaagtgcg caatttacgc gcgatggcga tggtgacatt tttctcccgc
    13381 gtaccaccgg ggccgatagc gccagggtcc tgaccgccgt gtccggcatc aatagcgatg
    13441 ataattttat cgccagtgtt agccgtcgcg cgcgctgccg gacgcgttac cgtattactg
    13501 ctgataacac ccgtagtgcg gttactttcc gttttaaacg gattgcgcgc cggttcgctg
    13561 acgcgcggtg cgacaaccgc aggcgtttca acgcgtttcg caaccacggg cggtggagga
    13621 ggcggtggcg gcacatcggc gttaatcgta aagacgacag tgtaattgct gccattctgc
    13681 cgcttcaccg cttcggtttt accgttttcg gtaagatcga ccaccagccg tagcgtttgt
    13741 gcatctttag gcgttccaga gcgaatcgcc ttcaccagat tattgccgct gaacaacaac
    13801 ggcagtccct gaatcacgcc cgtttgtttg atatcgagcg ccacggtgcg tttgctttga
    13861 tggctaaacg cataatcagg atcgccaata aaactcaacg ttatccgcgc ctgttgatta
    13921 ccgttagaaa cctgaatatc agagagcgtc gcggcaccca ccggcgtgca cagcagcagc
    13981 agcgtcgcta ccaaccaatt tctgatgcga tacatcatcc cgccaccttt caaaggttaa
    14041 ccggctaaac gcgccagcaa caattcaccc gcagaggaaa ccgcactcac gcgcgcctca
    14101 cggccttgtg cctgataatc aatgtgtatt tcgacatccg ggtcaggaag aacacctgta
    14161 ccttgttgtg gccactccac caggcagatg gcatcgttgg caaaataatc gcggatcccc
    14221 ataaactcca gctcctcggg atcggcaagg cggtacaaat caaagtgata aaccattaaa
    14281 ttgtcgagcg tatagggttc gaccagcgta taagtggggc ttttgacatt accctgatga
    14341 cccagagctt gtaaaaaacc ccggctaaag gtggttttac ctgcgcctaa atcgccatac
    14401 agatagatta cggttgcgcc atcgcaggct ttcgctaccc gctcgcccag gtctaatgtt
    14461 gcctgctcat cagggagcgg aattactcga ttcatcatgg tttttatcag tcacttccgg
    14521 gttaacaata cgctgtagcg tggaaaagag atcggttgcc agcatcccgc gcgttccaaa
    14581 acgcgccgcc agtacgtcag ctgccgcacc gtgcgcgaca cagcccgcac aggcggcatc
    14641 atacggcgac agtttttgcc caagcaatgc gccaataata ccagagagca catcgcccat
    14701 gccgccgctc gccatgcctg catttccggc atcaataatg cctaaagcgt caggatgggc
    14761 ggcgaccacg gttccggcac ctttcagcac cgctacgccg ccataacgtt gtaccagacg
    14821 tttggcgcaa tgtaagcggt cactttcaat ttcagcgacg gaacagccta acaaccgtgc
    14881 ggcctcgcca ggatgcggcg tgatcacgcg attgtgacgc ttatcgggat tgattgccag
    14941 caggttcaat gcatcggcat cccacaacat cggtttgcga aaattctcaa ctttttgcag
    15001 tgcttttttc ccccactctt gctggcccag accgggacca atcaccacca catcggccca
    15061 ttccaggctt tcggtaagag agtccatcgt cagttcatgc accatcaatt ccggtcgtgc
    15121 agtcagcagc ggcgcaatgt tttcactgcg ggtcagtact cggactaaac cagcaccagc
    15181 acgcagcgcc gcttcccccg tcatacgaat agcccccgcc gtgccgtgat cgccaccgat
    15241 aatcaccagc cgcccgtgat cgcctttatg cgaagtcggg cgacgcggtt tcagccagtg
    15301 agaaagttgt tctgccgaaa accgctgaat tttcgtctcc tgacctgcca gccagctatc
    15361 cagccccagt gagtcaaaat gcagttgtcc tgtaacatcc cgcgcttttc cagtaagcaa
    15421 gcctggtttc agcgcaataa aagtgatggt gtgatcggcg ttgatcactg cgcctggcgt
    15481 agcgccggtt tcagccagca ggccggaagg gatatcaacc gccacaatcg gcgcaggatg
    15541 ggaattagcg tggtcgatta actggctaat ggattcgcgg ggcgcttgct gcaagccggt
    15601 accgagcagc gcatcaacaa tcagatctac cgattcgggc cagacaatat tcgaagcatg
    15661 gatctcgcca cccgcgttta accatgcttc gcgtgccagc gcggcctctt ccggcaacgg
    15721 tttgtcgctc tcctgggcca acaacgtgac ctcaatgccg accgctttgg ccagtcgcgc
    15781 gaccacgtag ccatcgccgc cgttattacc atgaccgcac agcaccagcc agtggcgggc
    15841 gtcaggatac gccgaacgac acacctggaa tgcggcctcg ccagcgcgaa gcatcagctc
    15901 atagagtgtg agccccagca catctgccgc ctcgcgttct ccgcggcgga tatcgtcggc
    15961 gtaccagacg gtgtgtggta tacttacggg gtttttcttc attgtatggt ccgtcatgtc
    16021 agagcccctc gatctcaatc agttagcgca aaaaattaaa cagtgggggc tggaactggg
    16081 ctttcagcag gtaggtatta ccgataccga tctcagcgag tccgagccca aactgcaagc
    16141 atggctggac aaacaatacc acggcgaaat ggactggatg gcacgtcacg gtatgctgcg
    16201 cgctcgcccc catgagttat taccaggtac gctgcgtgta atcagcgtgc ggatgaatta
    16261 ccttcctgct aacgccgcat ttgccagcac gctgaaaaac cccaaactcg gctatgttag
    16321 ccgttatgcg ctgggccgtg actatcacaa acttctgcgc aaccgactca aaaagctggg
    16381 cgagatgatt cagcaacatt gtgtttcgct gaattttaga ccgtttgtcg attctgcgcc
    16441 tattctcgag cgcccgttag ctgaaaaagc tgggctcggc tggacaggta agcactcact
    16501 tatcctcaat cgcgaggccg gttctttctt ctttttaggc gaattgctgg tcgatattcc
    16561 gctgcccgtg gatcaaccag tcgaggaagg atgcggtaaa tgcatagcct gtatgacgat
    16621 ttgcccgacc ggtgccatcg tcgagccata taccgtcgat gctcgccgct gtatctctta
    16681 tctcaccatc gaacttgaag gggcgatccc ggaagagttg cgaccgttaa tgggaaaccg
    16741 tatttacggt tgcgatgact gccagcttat ctgcccgtgg aatcgctatt cgcaactcac
    16801 tacagaagac gatttcagcc cgcgtaagcc gctacacgca caggaactca ttgagttatt
    16861 tgcctggagc gaagagaagt ttttaaaagt cacggaagga tcggcgattc gccgtatagg
    16921 tcacctgcgc tggctgcgta atatcgccgt ggcattaggc aatgcgccct gggatgaaac
    16981 gattctggcg gcgctggaga gtcgcaaagg tgagcaccca cttcttgatg agcacatagc
    17041 gtgggcgatt gcgcagcaaa tcgagagacg aaatgcgtgc atagtcgaag tgcaattgcc
    17101 gaaaaaacag cgtctggttc gggtgattga aaaagggtta ccgcgtgacg cctgattcat
    17161 ccacagcttg tgaataaaaa taaaaacgca ttgcaattca agacgcataa aatcgtcaag
    17221 tgatcgactc gacaaaacgg aatcttgatt tttaacttat ttatcaatag gttaaaaaaa
    17281 taccattcac cgtgcgaagt ttctttgaaa ttaagattac agcctgagga tgtggataac
    17341 tctgtttaca agagtttgtc agagacaaca aaaaccagca ataacgcatc gcgtcgctgt
    17401 ggatatttta ttgagagaag aatttggagc gggaaacgag actcgaactc gcgaccccga
    17461 ccttggcaag gtcgtgctct accaactgag ctattcccgc ttgggtggtc tgtgccttac
    17521 agcactttca aattttggag cgggaaacga gactcgaact cgcgaccccg accttggcaa
    17581 ggtcgtgctc taccaactga gctattcccg cttaatcttc gtctttcgag ccgctactgc
    17641 cttggctgcc ttcactcacc ttagtcacat acttttgtaa gttcccaagg attcgttcag
    17701 ttgccgcctt gtcgcaattc gaaatactgc gattgggtgg tctgtgcttt gcagcacttt
    17761 tcaaattttg gagcgggaaa cgagactcga actcgcgacc ccgaccttgg caaggtcgtg
    17821 ctctaccaac tgagctattc ccgcgtacta cttaattttg ctttcgtaat tttcgcattt
    17881 ctgcgtcgtt acgggaggcg cattatacga gaaatctttc cctctgcaac cccccaaaaa
    17941 agtttttttt gcgtttttat ctcaagtgct gaaattatca gcaagacgtt caatttagcg
    18001 tcaaaatcac gccgccgcaa gtggattccg gcgcttacgt gaccaggaaa aattttacag
    18061 cttgataaaa tgctcgcggt agtaagccag ctccgccacc gattcacgga tatcatccat
    18121 tgcctgatgc gtcccctgct tggtaaaacc atccagaatt tccggcttcc agcggcgcgc
    18181 cagctctttc agggtgctga catcgagata acggtagtgg aagtaggctt ccagctccgg
    18241 catgtattta aacaggaaac gacggtcctg tccgatgctg ttaccgcaaa tcggcgattt
    18301 tcccgcaggc acccactgtt ttaagaattc gagcgttgcc agttcagctt cccgatcgcc
    18361 catcgtgctc gctttcacgc gctctaccag cccgctggcg gtatgggtgc gcacgttcca
    18421 gtcatccatc agcgccagct gttcatcgga ctgatgtact gcaatggtcg gcccttctgc
    18481 cagaatattc agattggcat cggtcaccag cgtggcaatc tcaataatgc gatcgcgctc
    18541 gggatccaga ccggtcatct caagatcgat ccaaatcagg ttgttttcat tggcactcat
    18601 gctattttcc acccattcac agtgactata ttgatccaca attgcgtgta tcatagatgt
    18661 tttgcccatc aggggcgacc aggagtcagt acgattgagt aaaaataaac tctccaaagg
    18721 ccagcagcgc cgcgtgaacg ccaatcacca gcgtcgtctt aaaacgtcta aggagaagcc
    18781 cgactacgac gacaatctgt ttggcgagcc tgatgaaggt atcgtcatca gccgctttgg
    18841 tatgcacgct gatgtggaat ccgccgatgg cgacgttcac cgctgcaata ttcgccgtac
    18901 catccgttcg ctggtaaccg gcgaccgcgt agtctggcgt ccgggtaaac cggcggcgga
    18961 aggcgtaaat gtcaaaggga tcgtggaagc ggtgcatgag cgtacctcgg tgttgacgcg
    19021 tccggatttc tacgacggcg taaaacctat tgccgccaac atcgaccaga ttgtcattgt
    19081 ctccgccatt ttgccggagc tgtcgctcaa tattatcgac cgttacctag tggcctgcga
    19141 aaccttgcag attgagccga ttattgtgct caacaagata gacctgctgg acgacgaagg
    19201 catggcgttc gtcaacgagc agatggatat ctaccgcaat atcggttatc gcgtattgat
    19261 ggtttccagc catactcagg atgggctaaa accgctggaa gaggcgttga ccgggcgcat
    19321 cagcattttt gccgggcagt ctggcgtcgg caaatccagc ctgctgaacg cgttgctggg
    19381 gctgcaaaaa gagatcctga ccaacgatgt ctctgacaac tcggggcttg gtcagcacac
    19441 cacgaccgcc gctcggctgt atcacttccc gcacggcggt gatgtgattg attcccctgg
    19501 tgtgcgtgag ttcggcctct ggcacctgga gccggaacaa atcactcagg gctttgtcga
    19561 attccatgac tatttaggtc tgtgtaaata tcgcgattgc aaacacgata ccgatccggg
    19621 ctgcgctatc cgggaagcgg ttgaggaagg gaaaatcgcg gaaacccgtt tcgaaaacta
    19681 tcaccgtatt ctggaaagca tggcgcaggt aaaaacgcgt aaaaactttt ctgatacgga
    19741 tgactgacaa ttaagctaac cgtcgttaga atcgtcccct ttttttcagg atccggcatg
    19801 taggccgaat caggaacgac aaaacaatgg cctggaggct accttgttaa attcatttaa
    19861 actttcgcta cagtacattc tgccgaaact atggcttact cgcctggcgg gttggggcgc
    19921 aagcaagcgg gcaggatggt tgacaaaact ggttatcgat ctgttcgtta aatactacaa
    19981 ggtcgacatg aaagaggcgc aaaagccgga caccgccagc taccgcacct ttaacgaatt
    20041 ctttgtccgt ccgctgcgtg acgaagtacg cccaatcgat accgatccga atgtactggt
    20101 catgcctgcc gatggcgtta tcagccagtt gggtaaaatc gaagaagata aaatcctgca
    20161 agccaaaggc cacaactaca gcctcgaagc cctgctggca ggcaactatc tgatggcgga
    20221 cctgttccgc aacggtacgt ttgtgaccac ttacctctcc ccgcgtgact accaccgcgt
    20281 acacatgccg tgcaacggta ttctgcgtga gatgatctac gtgccgggcg atctcttctc
    20341 cgttaaccat ctcacggctc agaacgtgcc gaatctgttt gcccgtaacg aacgcgtgat
    20401 ttgccttttc gataccgaat ttggcccaat ggcgcagatt ctggtcggag cgacgattgt
    20461 tggcagcatt gagacggtct gggcgggcac cattacgccg ccgcgcgaag gtatcatcaa
    20521 gcgctggacc tggcctgccg gggaaaacga cggttctgtg gcactgctga aaggccagga
    20581 aatgggtcgc tttaaactcg gttccaccgt tatcaacctg tttgcaccgg gtaaagtgaa
    20641 tctggttgag caactggaaa gcctgtctgt cacgaaaatt tgccagccgc tggcagtatc
    20701 taccgaaacc tttgttacgc cagacgctga acctgccccg cttcctgctg aagagatcga
    20761 agcagaacac gacgccagcc cattggttga cgacaaaaaa gaccaggtct aatccatcaa
    20821 aggaaacgct gacgtgcgcc tgattatcac ttttctgatg gcctggtgcc tcagttgggg
    20881 ggcgtacgcc gcgacggccc ccgatagcaa acaaatcact caggaactgg agcaggcaaa
    20941 agcggcgaaa cccgcacagc cggaagtcgt agaggcgctt cagtctgcct taaatgcgct
    21001 tgaggaacga aaaggttccc ttgagcgcat caaacaatat cagcaagtta tcgataatta
    21061 tccgaaactc tccgctactc tgcgcgcaca attaaacaac atgcgtgaca agccgcgcag
    21121 cgtgtcgccg ggaatgtcta ccgacgcgct gaatcaggaa attctccagg tcagcagcca
    21181 gttgctggat aaaagccgtc aggcccagca agagcaggag cgcgcccgcg agattgccga
    21241 ttcgctgaat caactgccgc aacagcaaac cgacgcccgc cgtcagttaa atgagatcga
    21301 gcgccgcctg ggaacgctta ccggcaatac tccgctcaat caggcacaaa atttcgcgtt
    21361 gcagtctgac tctgcacgtc ttaaggcgct cgttgatgaa ctggagctgg cgcagctgtc
    21421 tgccaataac cgccaagaat tagcgcgctt acgctcagag cttgcggaaa aagagagcca
    21481 gcaactggat gcgtatttgc aggccttacg taatcagttg aatagccaac gtcagcttga
    21541 ggcggagcgg gcgctggaaa gtaccgaatt actggcagaa aacagtgccg atttgccgaa
    21601 agatatcgtc gcgcaattca aaattaaccg cgaactatcg gcagcgctga atcaacaggc
    21661 gcagcggatg gatctcgttg cctcgcaaca gcgtcaggct gccagccaga cgttacaggt
    21721 ccggcaggcg ttgaatacgc tgcgtgaaca gtcgcaatgg ctgggatcgt ccaatctgct
    21781 cggtgaagcg ttgcgagcgc aggtggcacg gctaccagaa atgccaaaac cacaacagct
    21841 tgataccgaa atggcgcagt tgcgtgtgca acggttacgt tatgaggatc tgcttaataa
    21901 acagccgctg ctacggcaaa tccatcaggc cgacggtcag ccgctgaccg ccgagcaaaa
    21961 ccgtattctg gaagcacaac tgcgcactca gcgtgagttg ctgaactcat tgttgcaggg
    22021 tggcgacacg ctactattgg aactgaccaa gctgaaagtc tccaacgggc aactggagga
    22081 tgcgctgaaa gaggtgaacg aagcaacgca ccgctatctg ttctggacct ctgacgtgcg
    22141 tccgatgacc atcgcctggc cgctggaaat cgcccaggat ctgcgtcgtc tcatttcgct
    22201 ggacaccttc agtcagttgg gcaaagccag tgtgatgatg ctgaccagca aagagacaat
    22261 tttgccgctg tttggcgcgt tgattctggt cggttgcagt atttactcgc gccgctattt
    22321 cacccgtttt cttgaacgtt cggcggcgaa agttggcaaa gtgactcagg atcacttctg
    22381 gctgacgttg cgcactcttt tctggtcgat tctcgtcgcg tcaccgttac cggtgctgtg
    22441 gatgacgctg ggttacggct tgcgcgaggc gtggccttat ccgctggcgg tcgcgattgg
    22501 cgatggtgta acggccaccg tgccgctgct gtgggtagtg atgatttgcg ccacctttgc
    22561 ccgcccgaac ggcttgttta tcgctcattt tggctggccg cgcgaacgcg tatctcgtgg
    22621 gatgcgctac tacctgatga gcatcgggct tattgtgccg ctgattatgg cgctgatgat
    22681 gttcgataac ctcgacgacc gtgaattctc cggttcgctg ggaaggcttt gctttatcct
    22741 catttgcggt gcgctggcgg tggtcaccct cagcctgaaa aaggccggga ttccgctgta
    22801 tctcaacaaa gaaggcagcg gcgacaacat taccaaccat atgctgtgga acatgatgat
    22861 tggcgcgccg ttggttgcca ttctggcgtc ggctgtgggt tatctggcaa cggcacaggc
    22921 gctgttagcg aggcttgaaa cctcggttgc catctggttc ctgctactgg tggtttatca
    22981 cgttatccgc cgctggatgc tgatccagcg ccgcaggctg gcgtttgacc gggcgaagca
    23041 tcgccgggca gagatgttag cgcaacgtgc gcgtggcgaa gaggaagcgc atcatcacag
    23101 tagcccggaa ggagcaattg aagtcgatga aagcgaagtc gatctcgatg ccatcagtgc
    23161 gcaatccttg cggctggtgc gctcaatttt gatgttgatc gccctgcttt ctgtcattgt
    23221 gctgtggtca gaaatccatt ccgctttcgg cttcctcgaa aatatttcgc tgtgggatgt
    23281 cacctccacg gtacagggcg tagaaagtct ggagccaatt accctcggtg cggtgctgat
    23341 tgccattctg gtgtttatca tcaccacgca gctggtgcgc aacctgcccg cgctgctgga
    23401 actggcgatt ttgcagcacc tggatttaac gccgggtacg ggctacgcca tcaccaccat
    23461 caccaaatat ctgctgatgc tgattggcgg gctggtcggc ttctcgatga ttggtattga
    23521 gtggtcgaaa ttgcagtggc tggtcgccgc gctcggtgtt ggtctcggtt ttggtttgca
    23581 ggaaattttc gccaacttta tctctggtct gattatcctg ttcgaaaaac cgattcgcat
    23641 tggcgatacg gtgacaattc gcgatctcac cggtagcgtg acgaaaatta acacccgcgc
    23701 caccaccatc agcgactggg accgtaaaga gataatcgtg ccgaacaagg cgtttattac
    23761 cgagcagttt atcaactggt cgctctctga ctcggtcacg cgcgtagtgt tgacgattcc
    23821 ggcccctgct gatgccaaca gtgaagaagt gacggaaatc ctgctcaccg cagcgcgtcg
    23881 ctgctcgctg gtgatcgaca acccggcacc ggaagtcttc ctggtggatc tgcaacaagg
    23941 gattcagatt ttcgagctgc gtatttatgc cgctgagatg ggtcaccgta tgccgctacg
    24001 ccatgagatc caccagctga ttctggctgg cttccatgcc cacggtatcg atatgccatt
    24061 cccgcccttc cagatgcgtc tggaaagcct caacggtaaa caaacgggga gaacgctgac
    24121 gtctgcgggc aaaggtcgtc aggcgggaag tttgtaatgc atgaagctgc caaatgatgt
    24181 caacgtggct catttagcag cttaacatca tcttcagtta tcaccggccc tggagagtat
    24241 catccatacg cattacttcc tgacaactca tcgccggaga gacgatgagt tgcaggattt
    24301 catatgatca gtcgagagct aactcaggtt tattcgccat gcgactgcaa tagttctggt
    24361 aaatcgccat cgctaacagc gagaagaaga cagggccgcc aatcatccac aatgtgctgt
    24421 cccagtctcc ggcttcaacc acaggctgaa taatggtgaa gacgttcgca aatgtcacca
    24481 ccagtacgac aaccaccatc gcaatcattg ccgacatacg cgttttgaaa atcacaaatg
    24541 gtctgtcgag atcctgacgt gctttaaaga atgggaaagc cagcgcgagg aacaggtaag
    24601 gaagcgtcat agacacgttc gccatcagcg tcagcttgtt aaagaacgcg gatgcggtac
    24661 cgccaccaaa cgaaaccagc aggatgaaga cagtaaccaa cccgcactgc atccacatgg
    24721 cgatagacgg catccccatc gcattcaggc gcgtcatcgg ttccggccac aacgctttcg
    24781 gcgtcccctg gatgatggct ttcagcggtg aatagcaaag cgtaaagaac gcaccggtat
    24841 aagcgaggaa catcgaaagt ccggtaatac gcgcaaacca tacgcccagc gacaatgacg
    24901 cttcaggtga caaatgcagt gcgttaccca gcgtcacccc gaggctcttc atcagcacat
    24961 aggtaatatt gccaaggtta acggaaccat tacttaatac ctgctgccag tttgtgctga
    25021 cgccccataa aaatattgcc agcgaataac cgattgaaat aacaatagcg gcaaaaacaa
    25081 tacctttggc aaagttcttt tctggatttt ccgttttatc gaccagacca ccaaccgctt
    25141 caattccgcc ataggcaaaa atagcaaata ccacaaacga tagcattgcc agaccggact
    25201 gataacccgg gttcggtgat gcaaggaaat taatatcctg cgcgaaatgc ccaccattta
    25261 ataacaaaat agtaatgctt actaacagca atactaaatt cagacacatt actgcaatac
    25321 cgcccaccgc agtaatgcgg gcaattttat taatcccctt tgaagcaacg acggtgacca
    25381 gaatcatcca tgctactgcc agcagaccaa ccacctgcgt aggctccagt ccggcaatac
    25441 gccagtgctg ggtcatgtcg ctaccataga ggaatgttga gaacggtacc caaactttcg
    25501 cggaggtact caccatccag atgatataag aggaaaacca cataaacgta ccaatgaagg
    25561 caaaacgtgg tccgacacta ttattcatcc aggaatagat accaccttct tctttgcgat
    25621 aagcagctcc catttcagcc atcattaa
//
LOCUS       DAEEKQ010000047        25273 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.047, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000047 DAEEKQ010000000
VERSION     DAEEKQ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25273)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25273)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25273
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.047"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..748
                     /locus_tag="KLK57_001841"
     CDS             <1..748
                     /locus_tag="KLK57_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBE0892222.1"
                     /translation="IEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLM
                     RYATAAMQRHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAF
                     PLVDITIVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVKGFTNDSQLQTL
                     FNYLLQCGDTSRFTRFIEEISERSPLQKERLMTIAERLRQEGHQIGWQKGMHEQAIKI
                     ALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(789..1592)
                     /gene="yfaU"
                     /locus_tag="KLK57_001842"
     CDS             complement(789..1592)
                     /gene="yfaU"
                     /locus_tag="KLK57_001842"
                     /EC_number="4.1.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxy-L-rhamnonate aldolase"
                     /protein_id="HBE0892223.1"
                     /translation="MNALLTNPFKERLRKGEVQIGLWLSSTTSYMAEIAATSGYDWLL
                     IDGEHAPNTIQDLYHQLQAVAPYASQPVIRPVEGSKSLIKQVLDIGAQTLLIPMVDTA
                     DQARQVASATRYPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESKTALDN
                     LDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAAGFLAV
                     APDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(1610..2899)
                     /locus_tag="KLK57_001843"
     CDS             complement(1610..2899)
                     /locus_tag="KLK57_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416749.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0892224.1"
                     /translation="MSTALLDAVVKKNRVRLIPFMLALYVLAFLDRSNIGFAKQTYQI
                     DTGLSNEAYALGAGIFFVVYAFLGVPANLLMRKLGARTWIGTTTLLWGFLSAAMAWAD
                     TEAKFLIVRTLLGAAEAGFFPGMIYLTSQWFPQRNRASIMGLFYMGAPLALTLGSPLS
                     GALLEMHGFMGHPGWFWMFVIEGLLAVGAGVFTFFWLDDTPEQARFLSKQEKTLLINQ
                     LASEEQQKVTSRLSDALRNGRVWQLAIIYLTIQVAVYGLIFFLPTQVAALLGTKVGFT
                     ASVVTAIPWVAALFGTWLIPRYSDKTGERRNVAALTLLAAGIGIGLSGLLSPVMAIVA
                     LCVAAIGFIAVQPVFWTMPTQLLSGTALAAGIGFVNLFGAVGGFIAPILRVKAETLFA
                     SDAAGLLTLAAVAVIGSLIIFTLRVNRTVAQTDVAHH"
     gene            complement(2956..4160)
                     /gene="rhmD"
                     /locus_tag="KLK57_001844"
                     /pseudo
     CDS             complement(2956..4160)
                     /gene="rhmD"
                     /locus_tag="KLK57_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311162.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnonate dehydratase"
     gene            complement(4175..4957)
                     /locus_tag="KLK57_001845"
     CDS             complement(4175..4957)
                     /locus_tag="KLK57_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HBE0892225.1"
                     /translation="MLESSKVPALTRAIDILNLIARIGPCSAATIIDTLGIPKSTAYL
                     LLNELRRQRFLSLDHQENFCLWTRLVELSGHALSKMDLRELARPRLTQLMDTTGLLCH
                     LGIIDNGSAYYILKVESSATISVRSHEGKSLSLYRSGIGKCLLAWQPAAVQQSIIEGL
                     VWEQATPTTITHPQQLHEELARIRRQGWSYDNGEDYPDVRCVAAPVFNANNELTAAIS
                     VVGTRLQINEEYRDYLAGKAIACARDISRLLGWKSPFDLQAS"
     gene            complement(5177..6379)
                     /locus_tag="KLK57_001846"
     CDS             complement(5177..6379)
                     /locus_tag="KLK57_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HBE0892226.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVRLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQSPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(6479..7021)
                     /locus_tag="KLK57_001847"
     CDS             complement(6479..7021)
                     /locus_tag="KLK57_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892227.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIKSYEEANAGARYTIMHPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            7300..7725
                     /gene="nudI"
                     /locus_tag="KLK57_001848"
     CDS             7300..7725
                     /gene="nudI"
                     /locus_tag="KLK57_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HBE0892228.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(7764..8366)
                     /locus_tag="KLK57_001849"
     CDS             complement(7764..8366)
                     /locus_tag="KLK57_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HBE0892229.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            8674..9813
                     /gene="arnB"
                     /locus_tag="KLK57_001850"
     CDS             8674..9813
                     /gene="arnB"
                     /locus_tag="KLK57_001850"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HBE0892230.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTHRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            9817..10785
                     /gene="arnC"
                     /locus_tag="KLK57_001851"
     CDS             9817..10785
                     /gene="arnC"
                     /locus_tag="KLK57_001851"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HBE0892231.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            10785..12767
                     /gene="arnA"
                     /locus_tag="KLK57_001852"
     CDS             10785..12767
                     /gene="arnA"
                     /locus_tag="KLK57_001852"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HBE0892232.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPAEKAFYGS
                     VARLAAERGIPVYAPDNVNHPLWVERIAQLSPEVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGHRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISVAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQMLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NARRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            12764..13654
                     /locus_tag="KLK57_001853"
     CDS             12764..13654
                     /locus_tag="KLK57_001853"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HBE0892233.1"
                     /translation="MTKAGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            13654..15306
                     /gene="arnT"
                     /locus_tag="KLK57_001854"
     CDS             13654..15306
                     /gene="arnT"
                     /locus_tag="KLK57_001854"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HBE0892234.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGFTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            15303..15638
                     /gene="arnE"
                     /locus_tag="KLK57_001855"
     CDS             15303..15638
                     /gene="arnE"
                     /locus_tag="KLK57_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HBE0892235.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            15638..16024
                     /gene="arnF"
                     /locus_tag="KLK57_001856"
     CDS             15638..16024
                     /gene="arnF"
                     /locus_tag="KLK57_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HBE0892236.1"
                     /translation="MGLMWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(16018..16284)
                     /gene="pmrD"
                     /locus_tag="KLK57_001857"
     CDS             complement(16018..16284)
                     /gene="pmrD"
                     /locus_tag="KLK57_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HBE0892237.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCKVAGKTQ"
     gene            complement(16328..17749)
                     /gene="menE"
                     /locus_tag="KLK57_001858"
     CDS             complement(16328..17749)
                     /gene="menE"
                     /locus_tag="KLK57_001858"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HBE0892238.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     DYEIISFTSQFCHSIAVDNRFIC"
     gene            complement(17746..18708)
                     /gene="menC"
                     /locus_tag="KLK57_001859"
     CDS             complement(17746..18708)
                     /gene="menC"
                     /locus_tag="KLK57_001859"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HBE0892239.1"
                     /translation="MRSAQVYRWQIPMDAGMVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQIVLLAWVNNWLAGDGELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVLEQLL"
     gene            complement(18708..19565)
                     /gene="menB"
                     /locus_tag="KLK57_001860"
     CDS             complement(18708..19565)
                     /gene="menB"
                     /locus_tag="KLK57_001860"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HBE0892240.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNVGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(19580..20338)
                     /gene="menH"
                     /locus_tag="KLK57_001861"
     CDS             complement(19580..20338)
                     /gene="menH"
                     /locus_tag="KLK57_001861"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HBE0892241.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VYLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILNFWLVGYSLGGRVAMMAACQGMPG
                     LCGVVVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(20335..22005)
                     /gene="menD"
                     /locus_tag="KLK57_001862"
     CDS             complement(20335..22005)
                     /gene="menD"
                     /locus_tag="KLK57_001862"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HBE0892242.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     SGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALVLLRQVSAPLILIVVNNNGGQIFSLLPTPKSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETALADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(22094..23389)
                     /gene="menF"
                     /locus_tag="KLK57_001863"
     CDS             complement(22094..23389)
                     /gene="menF"
                     /locus_tag="KLK57_001863"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HBE0892243.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIKAREFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADNVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(23468..23773)
                     /gene="elaB"
                     /locus_tag="KLK57_001864"
     CDS             complement(23468..23773)
                     /gene="elaB"
                     /locus_tag="KLK57_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HBE0892244.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(23828..24289)
                     /locus_tag="KLK57_001865"
                     /pseudo
     CDS             complement(23828..24289)
                     /locus_tag="KLK57_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311182.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            24354..25271
                     /gene="rbn"
                     /locus_tag="KLK57_001866"
     CDS             24354..25271
                     /gene="rbn"
                     /locus_tag="KLK57_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HBE0892245.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPMEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         6114 a         6815 c         6315 g         6029 t
ORIGIN      
        1 tattgaaaaa agtttacgcg cttactactc ggatgttttg tggtccgtgg aaaccagcga
       61 cggtgacggc tatatctact gcgtgattga acatcaaagc tctgcagaaa agaatatggc
      121 ttttcggcta atgcgctatg ccactgccgc catgcagcgt cacctggata aaggctatga
      181 cagagttccg ctggtggtgc cattgctgtt ttatcatggc gaaacatcgc cctacccgta
      241 ctcacttaac tggctggatg agtttgacga tccgcaactt gcccggcagt tgtacaccga
      301 agcttttccg ttggtggata tcaccatcgt acctgacgat gagatcatgc aacatcggcg
      361 tatagctctg cttgaactga ttcaaaagca tattcgcgac cgcgatttaa tcggtatggt
      421 cgacaggatc accacgcttt tggttaaagg cttcactaat gacagccagc tacaaacact
      481 gtttaattat ctgctgcaat gcggcgatac ctcccgtttc acccgtttta ttgaggagat
      541 ttccgaacgt tcaccactac aaaaggagag attgatgact attgctgagc ggctacggca
      601 ggaaggacac caaattggct ggcagaaagg tatgcatgaa caagccatta aaattgcttt
      661 gcgcatgctg gaacagggca ttgatcgtga ccaggtgctc gcggccaccc agctaagcga
      721 agccgatctg gcagcgaata accactaatt aacacaggcc cacagccgat ccccatgggc
      781 ctttgatatc aataactacc ttttatgcgt ggcccatttt tgcctgattt aaacatcgcc
      841 agtcgttgat ccagggcatc gctgtagagc atcgtgtcaa cgccaacagc gacaaagttc
      901 gctccccacg ccaggcattg ctgcgccata tcaggagcca cagccagaaa accagccgct
      961 ttacccgcag cacggatccg ccgaatgctg gtttcaataa ttcgctgcac ttccgggtgc
     1021 ccggcgttat ccgggtagcc caacgacgca gaaagatccg ctggtccaat aaacacgcca
     1081 tcaatccctt cgacgtcgag gatctcgtcc aggttatcca gtgccgtttt actttccacc
     1141 tgcactaaca gacaaagcga atcgttgact tgcgccatgt aattctcaat gcgtccccag
     1201 cgcgcagccc gtgccacact ggccccaaca ccacgctcac cgtagggagg atagcgcgtg
     1261 gcagacgcca cctgacgagc ctgatcggca gtatcgacca tcgggatcaa tagagtttgc
     1321 gcgccaatat ccagaacttg tttaatcagc gatttactgc cttccaccgg acggatcacg
     1381 ggttggctgg catagggcgc taccgcctgt agctggtgat aaagatcctg aatggtgttt
     1441 ggcgcgtgct ccccgtcaat cagcaaccag tcataaccgg aagtagcggc aatttctgcc
     1501 atataagaag tagtcgagct taaccacaga ccaatttgca cttcgccctt gcgtaaacgt
     1561 tctttaaagg gattggttaa taatgcgttc atcgtgttcc ttaacctatt taatgatgtg
     1621 ccacgtcggt ctgcgcaaca gtacgattta cacgcagagt gaaaataatc agcgaaccga
     1681 tgaccgccac cgctgccagt gtcagtaatc ctgccgcatc gctggcaaat aacgtttctg
     1741 ctttcacgcg caggatcggg gcaataaacc cgcccactgc accaaacagg tttacaaaac
     1801 caatccccgc agccagcgcc gtaccggaaa gcagttgtgt cggcatcgtc cagaacaccg
     1861 gctgcacggc aataaacccg atagctgcaa cacacagcgc tacgatcgcc attactggag
     1921 aaagcagccc ggacagacca ataccaatgc ccgccgccag taatgtcagc gctgcgacat
     1981 tacgccgttc gccggtttta tcggaatagc gcggaataag ccaggtccca aacaaggccg
     2041 caacccacgg aatggcggtg accaccgacg ctgtaaagcc cacttttgtt cccagcaatg
     2101 ccgcaacctg ggtcggcagg aagaaaatta atccgtaaac cgccacctga atggtcaggt
     2161 agataatcgc tagttgccag actcggccat tacgcagcgc atcgctcagc cgagaagtca
     2221 ctttctgttg ttcttcactt gccagttgat tgataagcaa cgttttttct tgtttactca
     2281 gaaaacgtgc ctgctccggt gtgtcatcaa gccaaaagaa tgtgaatacc ccagcaccga
     2341 ctgccaacaa tccttcaatc acaaacatcc agaaccagcc gggatgcccc ataaatccat
     2401 gcatctccaa cagcgcgccg gaaagcggtg atcccagtgt taacgccagc ggtgcgccca
     2461 tatagaacag ccccataatg ctggcgcgat tacgctgcgg aaaccattgc gaggtgagat
     2521 aaatcatacc agggaaaaat ccggcctccg cagcaccaag cagagtgcga actatcagaa
     2581 atttcgcttc agtatcggcc catgccatgg ctgccgaaag aaatccccac agcagtgttg
     2641 tcgtaccaat ccaggttctg gcccccagtt tgcgcatcaa aagattcgcc ggaacaccca
     2701 gaaacgcata taccacaaag aaaatgcctg ctcccagcgc ataagcttca ttactcaacc
     2761 cggtatcaat ctggtaggtc tgtttggcaa aaccgatatt cgaacggtcg agaaacgcca
     2821 gcacatacag cgccaacata aacggaatta aacgcacacg gtttttcttc accacggcgt
     2881 caagcaaagc ggtgctcata aaaagctcct tagaatatgt gggcgttacc gttgtcacag
     2941 caacgcccgg agtgattagt ggctgtaggg gcgtttcaaa ttgcagtcac gattgagttc
     3001 gacgccaaaa ccgggtttat caagcactga tttatgaata cgaccattca ccggaaccgg
     3061 ctcattgagc agaatcgggt caaactgcgg acgcatcgtt gaacaatccg ggctggtcat
     3121 caggaattcg ctgaatggcg tattggtgaa ggtgatcacc gcatggtgag agtaaacaga
     3181 cgaaccgtgc ggcaccacca gttgccccct ggatttggcg attgcggcaa tttccaccag
     3241 cgtggttaaa ccaccgcacc agccaacatc cggctgcata atgtcgatac cggtttctga
     3301 aagcgtacga aaagattgca gtgtgccgtg gtgctcaccg ctggtgacca tcattccgac
     3361 tggcgcgttg cgtttcagtt cgcgataacc ttcatactgc tgtggcggca ggcactcttc
     3421 gatccatttc aggttatagg gcgcgcaagc gtgggccagt ttggtggcat agttcacgtc
     3481 ctgactcatc cagcagtcga gcattaacca gaaatcctca ccgcattttt cacgcatatc
     3541 cgcgaccata acggcatctt tgcggatccc cgcatcgcca tcatgtggcc cccagtgcgt
     3601 cggcattttg ccaccgataa agcccatctc ttttgccaga tccggacgcg cgcctgtggc
     3661 gtagaactga atctcatcac gaactgcgcc gcctaaaagt ttataaaccg gaagcccgac
     3721 cactttgccg aacagatccc acagagccag atcgacacag gaaatcgtgt tcatcaccag
     3781 gccaccagag ccggagtaat acagggtggc attgagcatt tgatcatgga tcagtttgat
     3841 atcactgaca catttaccct caatgaaacg gttaagatgt ttttcgacaa taaaacagcc
     3901 catttcaccg gctgtcgaaa cagcgaatcc ggtctggccg ttttctgctt cgacttcaac
     3961 aatcaacgtg ccaagaacgt taatgccaaa tgactggcgt gactgctcgt aatcgcggta
     4021 tttactcatc ggggtggcaa tatgatcgtc aatccagtga ttcgccccct ggtcgtgata
     4081 atcaccgcgc cagcaccttt ttctgctgtc gcaccgccag taaaccaggc gcgaacctgt
     4141 ttgatttttg gtagggtcat gatgttctcc attgttatga ggcctgtaag tcaaagggac
     4201 ttttccatcc caacagacgt gaaatatccc tggcgcaggc aatggccttg cccgccagat
     4261 aatcacggta ttcttcattg atttgtaagc gggtaccgac caccgagatc gcagcggtaa
     4321 gctcgttatt ggcgttaaac accggcgcag cgacacaacg gacatcgggg taatcttcgc
     4381 cgttgtcata gctccagccc tgacggcgaa tacgcgccag ttcttcgtga agttgctgtg
     4441 gatgagtaat cgttgtgggt gtcgcctgct cccagaccag cccttcgata atactttgct
     4501 gtaccgctgc aggttgccag gcaagcaggc atttacctat tccggagcga taaagcgaaa
     4561 ggctttttcc ttcatgggaa cgcacgctga tagtggctga tgactccact tttaaaatgt
     4621 aataagcgct gccgttatca ataatcccca ggtgacataa cagcccggtg gtatccatca
     4681 gttgcgtcaa acgcggtcga gccagttccc ggagatccat cttgcttaac gcatgaccag
     4741 aaagctccac cagcctggtc cacaaacaaa aattttcctg gtgatcaagg ctaagaaaac
     4801 gctgacgcct gagttcatta agcagcaaat aggccgtgct tttagggatt cccaatgtgt
     4861 caatgatcgt cgcagcacta caggggccaa tgcgggcaat caaattgaga atatcaatag
     4921 cccgtgtcag ggcgggaact ttgcttgatt ccaacatact ggactccagt cttagatact
     4981 ggaatcagtc tcgttgctca gggggagtaa aattgtgaag cgcatcaaat cccgctcctc
     5041 gtccagcgga ctggagaaat tacgcacaaa tttgtgaatc agtgcacgga ttttttctta
     5101 ttgagtacaa caggttggac aagttttgcc ggagggggaa tgtgccggtg cagtaaaccg
     5161 gcacagagtg gcggaatcag acagataagg tcatggactc aacaacctca atccagccat
     5221 gctcactggc gatatcctgg ccgtttaacc aacggcgcag catattcagt gccatcattg
     5281 cgcacacttc ctgacggata gccaggctgt agcgtgtagt gctgaaacgc acacgcagag
     5341 cgaaagtgcc gtctggcgtg gctagcgcaa agttgagatg ctcgttctcg aaacccgaaa
     5401 cagccagtgc cagcccggca aaatggttgg cccgccgttc tgtaatccag tgcgcagttt
     5461 gcgccagggt ttcttcctgt gaaggaacca cttcacacgc cagcaatgga gcacctgcgc
     5521 gagaaagttg caaagccaat aaaccaccgg tgaattgctc gctcaacgtc aggctgaact
     5581 ggcggttttg caattcgcga ctgatctgcg cgggcagtcc ttcggtgcct tcgaaaatca
     5641 cgctctgtcc ggcaacacgt ttaacgtcca gccacagttt ttccatcgcc tgttgctcgc
     5701 ttgccggtcc ggtgagtttc agttcgatga tcggcattga ggagcgatag cccattgtta
     5761 cgcccggcgg cagttgtaga gtgtccaggc tttgtgccag atcgctttcc gaacgaccaa
     5821 aggtagttaa acgcagacaa accggcgact gtggtaaaga aaaacgctcg cgcaggcgcg
     5881 gcaggatttc gtgctcgacc atcaccttaa attctgacgg tacgccggga gtaaagaaca
     5941 tcaggcaacg attaagccgc acggcaaaac cacaggcggt gcctaccggg ttattgataa
     6001 attcagcact ggcaggcagc tccgcttgtt tacggttgct cggtgccatt actcgtccac
     6061 gttcgtggaa atagcgttcc atctctttga gccaggcttc atgcagcacc aggccttcac
     6121 cttttgctgt cgcagcggcg agtgcactta aatcatcact ggtcggtccc agcccgccgt
     6181 taacgatcag cacatcggcg tgctgactac gttcgcgaag aatggtgact aagtcatcaa
     6241 ggttatcccc caccgtattg cggcgagata atggcaaccc ctgatgaaag aaaaaatcgg
     6301 ccagccaggc agcgttagtg tcaacgattt gcccgtgtaa cacttcatcc ccggtggata
     6361 acatttccac ttttaacatg gttatctcct gcttttgtta tcgaatcact ataacgcaaa
     6421 gggagataag gcgtagtaag aaactgaatg gcgcgacaga atgccgcgcc ggaagggatc
     6481 agaaactggc gttaacccca acatacgggc cgtcagccac ggcgttgtcg cggttaccgt
     6541 ctttacccga cagattcagg tagcgataac ccgcctcaat actgactgga tgcataatgg
     6601 tgtaacgcgc gccagcattc gcttcctcat aacttttaat accgctggag agtgaatccg
     6661 gagagtagta atactcgcca aacaaacgga agctgttgcc aattttccac tgcaaaccac
     6721 ctcctaccgc tgcggcataa ccttcatcgc cgtaattcgg gttggtgtaa acgccttttc
     6781 cgccaacggt cgccattaac ggcccgagag gcagattcaa ccccagcccc acgccagcga
     6841 cgtcaccgtc gtcgtcgtta tgtgtccagt taccgcttaa cgccaggccc gtcgattcag
     6901 tcccaaaacc gacaccaata ttggtgtatt ctttacccgc ctgaccgctg atgctcattg
     6961 cattgaccga tgcagaaacc aacagcattc cggccagacc tgctagcgca atttttttca
     7021 ttatcccctt tcccacaagc aattaatcaa aattcccaaa gctgcgggat tgtactgcct
     7081 ggcacagagg attcaatgag cgaaaaaagg gatctagtca cttattgtaa ctaaatactt
     7141 cttaaataat taaacgccga aaaaataaac acactttacc gatacacaca aaaataataa
     7201 taagtgcagt tatcatcgca aaagagatta ctgtcacttt cacatatttc tgagttaccg
     7261 tattctcatg gtttcttttt atttataagg aatacccctg tgcgacaacg gactattgta
     7321 tgccctttga ttcaaaatga tggtgcttat ttgctgtgca aaatggccga cgatcgcggc
     7381 gttttccccg gtcaatgggc gctttcgggt ggcggcgtgg agtctggcga acgaattgaa
     7441 gaggcactac gccgcgaaat tcgcgaagaa ctgggagaac agctgctttt gacagaaatc
     7501 acgccgtgga ccttcagcga tgatattcgc accaagacgt atgcagatgg tcgcaaggaa
     7561 gagatttata tgatttacct gatttttgac tgcgtttctg ccaaccgaga agtgaaaata
     7621 aacgaagagt ttcaggacta cgcgtgggta aaacctgaag atctggtgca ttatgattta
     7681 aatgttgcca cccgaaaaac gttacgtttg aaaggtcttc tttaacaatg atgacagcca
     7741 tcactggctg tcagattgaa aatttagtgg ttaacgaatt ccccatccag ataaactttg
     7801 cctttttcca catgcatgac taaaccatcc agataatcag gtttaaatgt cgcatcacgc
     7861 ttatttttag caatatatgt caggcaatga ttatgggtga aaataacgat atttttatca
     7921 ggcgctttgc tttgtaagtc cttaattgca ctataaatct cattaccgca ctgaagaagt
     7981 cgtttatcta ccgtcagttt tttacccgcg gaaaaccagg tcgccgactg aatggtccgg
     8041 acggtattac tggaataaag atcgaaatca gggatgtcag cgctaaaagc gttgcccagt
     8101 tcacgggcat cctgggtacc tttaaccgta atacctgttt tatctgacaa gcattgattg
     8161 gttgaacgat cgcaacgttc agcatggcga aacaaaacga caaccgggtg ctgctgtgcc
     8221 agtctggcca atgttttatt gtcgatacgt ggcaaaccat tgctactcca ggcggcatga
     8281 gtacccagtc cggcaattgc agcgagcgcc agtaaaatga tgaaatattt ttttgacttc
     8341 aatgatgagc ggcaaaaagc taacacgtaa actccaccta tagacaagcg caaccagaca
     8401 attaccgtga aattgagcga catttctggc gataattagc agttggtgta atattaaaaa
     8461 tcctacgatg tcggcaaaat gcctcaaaat tttgccaaat gcaaagccta aataagaaaa
     8521 aatataaaaa tttcaatatt tacgtctaat attagtttct taaggttaag ttaatattct
     8581 atccttaaaa tttcgctcca aatggcaaaa tatacacaac actctttata gcaaatataa
     8641 gtggacaggt attcaatggc ggaaggaaaa gcaatgtcag aatttttgcc tttttcgcga
     8701 ccagcaatgg gcgtggagga actcgctgca gttaaagagg ttctcgaatc cggttggatc
     8761 acaaccggtc cgaagaatca ggcgcttgag caagcctttt gccagttgac gggaaatcag
     8821 catgccatcg cggtcagttc agccaccgcc ggaatgcata tcacgctaat ggcgttggaa
     8881 attggcaagg gcgatgaagt gattacgcct tccctgacct gggtttcaac cctcaatatg
     8941 atttccttgt tgggtgcaac gccggtaatg gtggatgtcg accgcgatac gctgatggtc
     9001 acgcctgaag cgatcgagtc agccattacg ccacgcacta aagccatcat tccggtgcat
     9061 tatgccggtg cgccagcaga tattgacgcc attcgcgcca ttggcgaacg ttacggcatc
     9121 gcagttatcg aagatgctgc ccatgccgtc ggtacgtatt acaaagggcg acatattggc
     9181 gcaaaaggta ccgctatttt ttcatttcat gccattaaaa atattacctg tgcggaaggt
     9241 ggcctgattg taactgataa tgaaaacctt gcccgccagc tacggatgct gaaatttcac
     9301 ggtctgggtg tcgatgccta tgacagacaa acctggggcc gtgcaccgca ggctgaagtc
     9361 ttaacaccgg gctataagta caatctgacc gatattaacg ccgcgattgc cctgacacag
     9421 ttagcaaaat tagagcacct caacacccat cggcgcgaaa ttgcccagca atatcagcaa
     9481 gcactggcag ctctcccctt tcagccatta agccttcccg cctggccgca cgttcacgcc
     9541 tggcatctgt ttattattcg tgtcgatgaa caacgttgtg gtatcagtcg cgatgcgttg
     9601 atggaagcgt taaaagaaag aggcattggt accgggttac atttccgcgc cgctcacaca
     9661 caaaaatatt atcgcgagcg ttttcccacg ctgtcgttac cgaataccga atggaatagc
     9721 gaacgcatct gttctttgcc gctgttcccg gatatgacta ccgccgatgc cgaccgtgtc
     9781 atcacagccc ttcagcaact cgcaggacaa taagccatgt ttgaaatcca ccctgttaag
     9841 aaagtctcgg tggttattcc cgtttataac gagcaggaaa gcttaccgga attaatcagg
     9901 cgcaccacca cagcctgtga atcgttgggg aaagagtatg agatcctgct gattgatgac
     9961 ggcagtagcg ataattccgc gcatatgctg gtcgaagcct cacaagcgga gaacagccat
    10021 attgtgtcta ttttgcttaa ccgcaattac gggcaacatt cagcgattat ggcgggattc
    10081 agtcacgtta ctggcgactt aattattacc cttgatgccg atctccagaa tccgccagaa
    10141 gaaatccccc gcctggtggc aaaagccgat gaaggttacg acgtggtagg gactgtacgc
    10201 cagaaccgcc aggacagctg gtttcgtaaa acagcttcga agatgattaa caggcttatt
    10261 cagcgcacca ccggcaaagc gatgggtgac tacggttgta tgctgcgcgc ctatcgccgt
    10321 catattgtcg atgcgatgtt gcactgccat gaacgtagca cctttatccc gattctggcg
    10381 aatatcttcg cccgccgtgc cattgaaatt ccagtacatc atgccgagcg tgagtttggt
    10441 gaatccaaat acagttttat gcgcctgatt aatttgatgt acgacctggt gacctgcctt
    10501 accacaacgc cgctacgtat gctgagtctg ctcggcagca ttattgcgat tggaggtttt
    10561 agcattgcgg tgctgctggt gattttacgc ctgaccttcg gaccacaatg ggcggcagaa
    10621 ggcgtcttta tgctatttgc cgtgctgttt acttttattg gcgctcagtt tatcggcatg
    10681 ggattgctcg gtgaatatat cggcaggatc tacaccgatg tccgcgcccg cccccgctat
    10741 tttgttcagc aagttatccg tccatccagc aaggaaaatg aataatgaaa accgtcgttt
    10801 ttgcctacca cgatatggga tgcctcggta ttgaagctct gctggctgcc ggttacgaaa
    10861 ttagcgccat ttttacccat accgataatc ccgctgaaaa agccttttat ggttcggtgg
    10921 ctcgtctggc ggcggaaaga ggcattccag tttatgcgcc ggataacgtt aatcatccgc
    10981 tgtgggtgga acgcattgcc caactctcac cagaggtgat tttctctttt tattatcgcc
    11041 atcttatttg cgacgagatt ttgcagctcg ctcccgcagg tgcatttaat ctgcacggtt
    11101 cgctgttacc aaaatatcgt ggtcgcgcgc cgctgaactg ggtgctggtg aacggtgaaa
    11161 cggaaacggg cgttacattg caccgaatgg taaaacgtgc cgatgctggg gccattgtgg
    11221 cgcaactgcg cgttgccatt gcgccagacg atatcgctat tacgctgcat cataaattgt
    11281 gccatgccgc gcgccagcta ctggaacaga cattacccgc cattaaacac ggtaatattc
    11341 tggaaattgc ccagcgcgaa aacgaagcca cctgttttgg tcacagaacg ccagatgaca
    11401 gtttccttga atggcataaa ccggcatccg tactgcacaa catggtacgt gccgttgccg
    11461 atccgtggcc gggtgccttc agctatgttg gcaatcagaa attcaccgtc tggtcgtcgc
    11521 gtgttcatcc tcatgccagc aaagcacagc cggggagcgt gatttctgtt gcgccactgc
    11581 tgattgcctg tggcgatggc gcgctggaaa tcgtcaccgg acaggcgggc gacggcatta
    11641 ctatgcaggg ctcgcaatta gcgcagatgc tgggcctggt gcaaggttca cgcttgaata
    11701 gccagcctgc ctgcaccgcc cgacgccgta cccgggtact catcctcggg gtgaatggct
    11761 ttattggcaa ccatctgaca gaacgcctgc tgcgcgaaga tcattatgaa gtttacggtc
    11821 tggatattgg cagcgatgcg ataagccgtt ttctgaatca tccgcatttt cactttgtcg
    11881 aaggcgatat cagtattcat tccgaatgga ttgagtatca cgtcaaaaaa tgtgatgtcg
    11941 tcttgccgct ggtggcgata gccacgccga ttgaatatac ccgcaacccg ctgcgcgtat
    12001 ttgaactcga ttttgaagag aatctgcgca ttatccgcta ctgcgttaag taccgtaagc
    12061 gaatcatctt cccgtcaact tcagaagttt atgggatgtg tagcgataaa tacttcgatg
    12121 aggaccattc taatttaatc gtcggcccgg tgaataaacc acgctggatt tattcggtgt
    12181 caaaacaatt acttgatcgg gtgatctggg cctatggcga aaaagagggt ttacagttca
    12241 ccctcttccg cccgtttaac tggatgggac cacgactgga taaccttaat gcagcgcgaa
    12301 ttggcagctc ccgcgctatt acgcaactca ttctcaatct ggtagaaggt tcaccaatta
    12361 agctaattga tggcggaaaa caaaaacgct gctttactga tatccgggac ggtatcgagg
    12421 cgttgtaccg cattatcgaa aatgcgggaa atcgctgcga cggcgaaatt atcaacattg
    12481 gcaatcctga gaacgaagcg agcattgagg aactgggcga gatgctgctg gcgagcttcg
    12541 aaaaacatcc gctgcgccat cacttcccac cgtttgcggg ctttcgtgtt gtcgaaagta
    12601 gcagctacta cggcaaagga tatcaggacg tagagcatcg taaaccgagc atccgcaatg
    12661 cccgccgctg cctggactgg gaaccgaaaa ttgatatgca ggaaaccatc gacgaaacgc
    12721 tggatttctt cctgcgcacc gttgatctta cggataaacc atcatgacca aagccggctt
    12781 acgcattgat gtcgatacct ttcgtggcac ccgtgaaggc gtgccacgtc tgctggaaat
    12841 cttgagtaag cataatattc aggccagcat ttttttcagc gtcggcccgg acaatatggg
    12901 ccgccatctc tggcgactgg tgaagccaca gtttttgtgg aagatgctgc gctcaaacgc
    12961 ggcatcgctt tatggctggg atattttact ggcaggtacg gcctggccag gtaaagagat
    13021 tggtcatgcc aatgccgata tcattcgtga agcggctaaa catcacgaag tcggcctgca
    13081 cgcctgggat caccatgcct ggcaagcccg tagcggtaac tgggatcggc aaacaatgat
    13141 cgacgatatt gcacgcggtc ttcgcactct ggaagagatt atcggtcaac cggtaacctg
    13201 ttctgccgct gcgggctggc gtgccgacca gaaggtgatc gaagcaaaag aagcgttcca
    13261 tttgcgctac aacagcgatt gtcgtggggc catgccgttc cgtccactgc tcgaatcagg
    13321 aaaccctggc actgcgcaaa ttccggtgac cttacccacc tgggatgaag tgattggtcg
    13381 ggatgtgaaa gcagaagatt ttaacggttg gttactcaac cgcatcctgc gagataaagg
    13441 cacgccggtt tataccattc atgcagaagt tgaaggctgc gcttatcagc ataattttgt
    13501 ggatctcctc aaacgcgcag ctcaggaagg cgtgacattt tgccctttaa gcgaactgtt
    13561 atcagagacg ttgccgctcg gacaagttgt tcgcggaaat attgccggac gtgaaggctg
    13621 gctgggttgc caacaaattg cgggtagtcg ctgatgaaat cggtacgtta ccttatcggc
    13681 ctcttcgcgt ttattgcctg ctattacctg ttaccgatca gcacgcgtct gctctggcaa
    13741 cccgatgaaa cgcgttatgc ggaaatcagt cgagaaatgc tggcatccgg cgactggatt
    13801 gtgccccatc tgttagggct acgttatttc gaaaaaccca ttgccggata ctggattaac
    13861 agcattgggc aatggctatt tggcgcgaat aactttggtg tgcgggcagg cgttatcttt
    13921 gcgaccctgt taactgccgc gctggtgacc tggtttactc tgcgcttatg gcgcgataaa
    13981 cgtctggctc tactcgccac agtaatttat ctctcattgt ttattgtcta tgccatcggc
    14041 acttatgccg tgctcgatcc gtttattgca ttctggctgg tggcgggaat gtgcagcttc
    14101 tggctggcaa tgcaggcaca gacgtggaaa ggcaaaagcg caggattttt actgctggga
    14161 atcacctgcg gcatgggggt gatgaccaaa ggttttctcg cccttgccgt gccggtatta
    14221 agcgtgctgc catgggtagc gacgcaaaaa cgctggaaag atctctttat ttacggctgg
    14281 ctggcggtta tcagttgcgt actgacggtt ctcccctggg gactggcgat agcgcagcgg
    14341 gagcctgact tctggcacta ttttttctgg gttgagcata ttcaacgctt tgcactggat
    14401 gatgcccaac atagagctcc gttctggtac tacgtgccgg tcatcattgc cggtagcctg
    14461 ccgtggctgg gattactccc cggtgcactg tacacaggct ggaaaaaccg caagcattcc
    14521 gcaaccgtct atttgttgag ctggacgata atgccgctgc tgtttttctc cgtcgctaaa
    14581 ggtaaattgc ccacctatat tctttcctgc tttgcatctc tggcaatgct gatggcgcat
    14641 tacgctttgc tggcagcaaa aaataatcct ctggcgctgc ggattaatgg ctggattaac
    14701 atcgcttttg gcttcactgg cattattgcc acatttgtgg tctccccgtg gggaccaatg
    14761 aacacgccgg tgtggcaaac cttcgagagc tataaagtct tttgtgcctg gtcgattttt
    14821 tcgctatggg catttttcgg ctggtacacc ttaacaaacg tcgaaaagac ctggtctttt
    14881 gccgcgcttt gcccgctggg gctggcgttg ctggtaggat tttcaattcc tgacagagtc
    14941 atggaaggaa aacatccgca attttttgtc gagatgacac aagaatcact tcagccaagc
    15001 cgctatattc ttactgatag cgtcggtgtt gccgcaggtc tggcatggag cctgcaacgc
    15061 gatgacatca tcatgtatcg ccagacaggt gagttgaaat acggccttaa ttatccggat
    15121 gcgaaaggga gatttgtcag cggtgatgag ttcgcaaact ggcttaatca acatcgtcag
    15181 gaggggatta ttacactcgt gctttcggtt gaccgcgatg aagatatcaa cagtctcgcc
    15241 attccgcccg cagatgccat cgatcgtcag gagcgtctgg tgctgattca gtatcgtccc
    15301 aaatgatctg gctaacatta gtctttgcca gcttgcttag cgttgccggg cagttgtgtc
    15361 agaaacaggc aacctgcttt gtggcgataa acaaacggcg caaacatatc gtgctgtggc
    15421 tgggactggc gctggcttgt cttggtcttg ccatggtgct ctggctgctg gtcttgcaga
    15481 acgtaccggt aggcattgcc tacccgatgt taagtctgaa ttttgtctgg gtgacgctgg
    15541 ctgcagtaaa actgtggcac gaaccggtat cgccgcgtca ctggtgtggg gtggcgttca
    15601 ttattggcgg cattgtgatc ctcgggagta cggtgtaatg ggcctgatgt ggggattatt
    15661 cagcgtcatt attgcctcag ttgcgcaatt aagcctgggt tttgcggcga gtcatctgcc
    15721 gccgatgacg cacttatggg attttatcgc ggctctgtta gcttttggcc tggatgcccg
    15781 gatactgtta ctcgggctgc tgggttatct gctttccgtt ttttgttggt ataaaacgct
    15841 acataaactt gcgctaagca aagcctatgc cttgctcagt atgagttatg tgctggtatg
    15901 gattgcttca atggtacttc ccggctggga agggactttt tcgctgaaag cactactggg
    15961 agtagcctgt attatgagcg ggttgatgct gattttcctg cccacgacaa aacaacgtta
    16021 ctgagttttc cctgccactt tacactgccg ttcccactca tcggggctat aactgcttgc
    16081 cgagaggact ttcaccgtat gcagcttttc acgattaata caataaagcg cattctgcaa
    16141 aggcgagagt aaatccccca ctttcacggc gaagtcactc ttcacttcgg cgatcatttt
    16201 tatcgcgccg ccagcatcgc agagcataag tacatgtctg ttatcttgtt tattgcagca
    16261 cgattttttg accagccatt ccattgcatt atcctgtttg ctaagagttt tcacatcaat
    16321 ttacagatta acagataaac ctattatcaa cggcaatgga atggcaaaat tgcgaagtga
    16381 aactaatgat ttcgtaatcc tggcgttgca cccactcttt taacgcctga cgtgaaattt
    16441 taataccgcc gtttttcagc tccggcggta aagttagcca gcgcaccggt tgttgaaaac
    16501 gagccagctt atctttcacc cattcgccaa gatcaacggt ttgctggtca tactccacca
    16561 ccgctaccgg tcgatgacca aactccttgt cggcaacggg gacgataaac acctgcaaaa
    16621 ccgcaggatg tgcagcaatt acgcgctcga cttcttccgg ctgaataccc tctccgccac
    16681 tgaagaacag attgtctaaa cgtccgacaa tggtcagctt gccattatgc atctcaccgc
    16741 gatcgcgcgt agcgtaccag ccttcgtcat taaccagtga aaccagttgc ccgttacgcc
    16801 agtaaccttc tgccatactg gcagcccgca gccacacttc attattaacg attttcactt
    16861 cccgacccgg cagcggcgaa ccaacgtctg ccaggccgtc ggcttctttc gcacacaccg
    16921 tggaggcaaa ctcggtcaga ccatagccgc aaaagcaacg aatcccctgc tcgcgcgcct
    16981 gttccgtcaa ctcgaccggg atagccgcgc cgccaagtaa caccgctttc agggaaacgg
    17041 aactgcggtt aaccagcaaa cgccagagtt gtgttggcac cagtgaagcg tgagtacagc
    17101 ctgccagcat ttgctccaat ggctgtttat cacgcaccgt catccgcgca ccagcgtata
    17161 accagcgcca cataattccc tgaccggaga cgtgaaacag cggtaaagag agcaaccagt
    17221 catcgtgatc gccaaacgga atcagcgata acacaccttg cgcactggca agatgggcct
    17281 gataagtatg gacagcggct ttcggcaaac cggtagaacc cgaggtcaac gtcattgagc
    17341 acagacgcgt cggctgccac gtagcggcat gtgcgccttc aacccgctga atgtgcagcg
    17401 acgttaatgc cggaaacgtg ttttccccat ccggcaccag cgcaaattgc agcgtcagat
    17461 tgggcagcaa ttcttcaagc aacggttgcg gcagctgagg gttcacgggc aacacccgcg
    17521 ccccgcattg cagtaacgcc agccaggcga gcagcgtttg cggcgtattc cacgcccgca
    17581 acatcacgcc gctgccctca accacaccct gcgccgcaaa gcccgtagct aactcatcga
    17641 cgcgagcaca aagctcgcgc cagttgagtt gctcgtcatt aagacgtaag gcgatggctt
    17701 ctccccgcac ttgccgccag tgacgccacg gccagtcaga gaagatcata acaactgctc
    17761 cagtacatca gcgtccacta acggcagtgg gctccccggc cagcgacgaa cctgctgcgc
    17821 ctgcatcaga tccagcgtgt ccagtcccgg aatggtgtct ggcgttaacc aggcagcaat
    17881 ccgcgccagt tgcgttagac ctaagctcga ctcaatggaa gaactgatca ccgccgtcag
    17941 ccccagcgca tgcgccgcct gtacctgctc gcgtactttt tccagactgc ccgtgagcgt
    18001 gggtttgata actaccgcgc gcacgccctc ttcagccaca aaggcaaaat ccggctcgcg
    18061 caggctttca tcccaggcaa tggcaatgcc ggtttcacgg gcaaacgctc gcgaatcatc
    18121 gcgggttttg cacggctctt cgagaaacgc gatgcggtgg cgataatccg ggttaacata
    18181 tttggcaaac tgctgacctt tcagcggtgt ccaggcgcga tttgcgtcaa gacgcaaatg
    18241 cagatccgga attgcctcca gcaacagatt aaccaccatg ccgtcgcgca cggcttcgta
    18301 caatcccact ttgaccttcg ccactttctc gcctggcata tcagcaagtt tgaggatcag
    18361 atcgtcagga tcgccattac acagtggtgc cgcacggtaa ttggccgcct gcgggagcgt
    18421 atctgccagc tccgccaatg cacagcttac gccaaaggca acggaaggca tctgcggtag
    18481 ctcgccatcg cctgccagcc agttatttac ccaagcaagc agcacaattt gcgcctcttc
    18541 ccaggtttcc tgactgaagc ccggcagtgg ggagatctcc ccccaccctt cgcgctcgcc
    18601 ttcacgcagg caaacataca gcccgtcgcg ggtttttaac cgcctgtcgc gcagaaccat
    18661 ccccgcgtcc atggggatct gccagcggta tacctgcgcg ctacgcatta cggattccgt
    18721 ttaaatttgc tgaaatcagg ctgacgtttc tggttgaaag cgttgcgacc ttcctgacct
    18781 tcttccgtca tgtagaacag catggtggcg ttgcccgcca gctcctgcag acccgcctga
    18841 ccgtcacagt cggcgttcag cgcagctttc aggcagcgca gcgccatcgg gctgttttgc
    18901 agcatttcgc ggcaccaacg gacggtttct ttttccagat ccgccagcgg taccacggtg
    18961 ttgacaaggc ccatatccag cgcctgtttt gcatcgtact gacggcacag gaaccagatt
    19021 tcacgcgctt ttttctgtcc gacgatgcga gccatgtagg aagcgcccca gccgccgtcg
    19081 aaggaaccga ctttcgggcc agtctgaccg aagatggcat tatctgccgc gatagtcagg
    19141 tcgcacatca tgtgcagaac gtgaccaccg ccgatggagt agccagccac catcgcgaca
    19201 accggtttcg gacaggtacg gatctgacgc tggaagtcca gcacgttcag gtgatgtacg
    19261 ccggaatcat ctttatagcc gccgtaatca ccacgcactt tctggtcacc accggagcag
    19321 aacgctttat cgcctgcgcc agtcagaatg atcacgccga cgttgtcgtc ataacgcgca
    19381 tctgccagtg cctggatcat ctcttttacc gtcagaggac ggaaggcatt gcgcacctgc
    19441 ggacgattaa tggtgatttt tgcgataccg tcggtggatt tttcataacg aatgtcctca
    19501 aaaccttcgg agcagtcgtg ccattcaacc ggtgcgtaaa gcattgcttc atcaggataa
    19561 atcatattgt gtcctttagt cagaaacgca agatctgcgc cagacttgcg ataacgccag
    19621 cgggattttc ccgatgcgcg ttatgtccgg cgcgaggaat gacatggcag tcggcagcca
    19681 gttccgccgc caaggcgcgg aatttgctgt cacgttcacc acataaataa taaaacgcaa
    19741 atgtgcgggc gctaaggtta gcacgtaaat caggctggac ggcgagagaa gtcgcctcca
    19801 gcatggcggc aagggttgcg ccattattgt tactgcgcag cgccaccagc tcccggcgtt
    19861 gatcgtcatt gagcgaggca aaaacaggct gttgatacca gtcggcaaat accgccgtta
    19921 acggttctgt gcgaaaacgc tgcgcccatt ggcgatcgga acgctgacgt tccgcgcgtt
    19981 gttcagcatt ttgcaaccca ggatgcccgc cttcgacaac aaccccacaa agccccggca
    20041 tcccctggca agccgccatc atcgccaccc gtcccccaag cgagtacccc accaaccaga
    20101 agttaaggat gttgtaacta accagggttt tacgcagtaa gtcggtgaca tcatcaaatc
    20161 catcgacgct aatcgccgcc gaaccaccgt gacctgggag ataaacatac aaccgtgagt
    20221 agtcggcaaa cgcctcgccc acttcttgcc attcgtggca atcgccggaa aaaccgtgca
    20281 aaaacaccag ccagggtaaa cctggtttgc cgtgttttgc ctgcgcgtgc aggatcataa
    20341 atggcttacc tgcgccagaa gttgctggag cgtttgcgca ccatcggtgt cgttaaccac
    20401 catttcaatt accgtggtgg ttggcgtgcg ccaggcatcg gcaagtgccg tttcaagttc
    20461 ctgccagttt tgcggacgat gatatttcag ctcgaacatc gcggcggcgt gctcaaaatg
    20521 gacgttttgc ggcatcagat agaaacgctc gcgctcgctt ttcggcgttg gcagcagcga
    20581 gaaaatttgc ccgccgttgt tgttcaccac aattaatatc agcggcgcgg aaacctgacg
    20641 caataacacc agcgcgttga gatcgtaaag tgcggagaga tcgcccacaa tcgccagcgt
    20701 cggtttgccg cttgcccgct gaacgccggc ggcagtcgag agcagcccgt cgataccgct
    20761 ggccccacgg ttgctgtaca ccgggtaacc tgccggaagt tgcgaaagcg catcaatcag
    20821 acgtaccacc aggctgttac cgacaaacaa ttgcccctgt tcaggcagat agtcgctgat
    20881 gcgatgcgcc agttgcgctt cgccaaacgc atcgcgacgg gcaataaccg cctgcattgc
    20941 ctgttccgcc aggcgcggga tttcaacgca ccagggctgg cgtttttctg ccggatgcag
    21001 ctccagccag tcggcaatat tggcaattaa gcggcgtcca cgatggtgcg ccggatcaag
    21061 ccgcccttcg atgtcatcca caatccagta ctcttcgggt tcacagcttg cctgccattg
    21121 caggagccgt ttgcccgtca ggctgcttcc cagttgcacc acaatttgcg cctgttgcag
    21181 ttcgctggtc gctttggcat tgcctaacca gagatcggca cacggcagcg gctgtccggt
    21241 ttgcgacagc acgtcgccaa tcagcggcca gccaagagtt tgcgcccaca gtgcaacttt
    21301 tttgccctct tccgcactca tgcgcccggc aaccaccacg ccgcgttttt gtcgccagaa
    21361 gaaccagtcg cgctgttttt cactttccag acgaggcgct tcacgcagcc acggtttgtc
    21421 gtcctgccac cagtcgccca gccgctgttg ccagctaagc ccggtgtcgt ccatttcgcc
    21481 atacagcggt tcggcaaacg ggcagttgat atggactcca ccagaatgaa gcgtaccgag
    21541 agcatggtcg atggtagaaa ccagccaacg tgcggggata tcctgggtcg ggcgcggcaa
    21601 tgaaatactg tgcgtggggt gagaggcgaa cattcccggc tgacgaatcg cctgattcgc
    21661 gccgcagtca atcagctccg gcgggcgatc ggcggttaac aggatcagtt tttctccggt
    21721 taacccagct tcaatcagtg ccggatagag atttgctacc gccgtaccgg aggtcacaat
    21781 caccgccacc ggctgcttgc tgactttcgc cagccccagc gccagatgcc ccaacccacg
    21841 ctcatcgaaa tgggtgtggt gaatgaatgc ggaattctcc gccgccgcta acgttaacgg
    21901 tgtagaacgc gagccagggg cgatacagat gtgtctgacg ccgtgacgcg ttaatgcttc
    21961 cagaatgacc gccgcccagc gtcggttaaa tgcgcttact gacatgagtt tgtccggtat
    22021 caatattgcg gctaagtata aggagctaca aaaatagatt attgatatga atcggtaatg
    22081 atgcgactca ttactattcc atctgtaata aagtacgcag ccctgccgct ttgttgtcga
    22141 tttcctgcca ctcttgctcg gggtcggaac cacggacaat gcccgcgcca gcatataatc
    22201 gcacgacatt gccgctaatt tttgctgagc gcagggaaac gcagaattcg ctttgctgta
    22261 atgagagata gcccgcagaa ccggcgtacc attctcgggt gaacggttcg tgacgggcga
    22321 taaactgtcg cgccagatcg cgcggtaagc cagcaactgc tgccgttggc tgcaactgat
    22381 gtaaacagat cacattatcc gctttgttga gtgaagtcca gatacagcgg cgaagatgct
    22441 gcactttacg cagacgcagt acctgcggcg gtaaaacatc cagcgtctgg gtatcggcct
    22501 gtaaccgttg gcagatatct tccaccacca gcatattctc gcgctggttt ttatcatccg
    22561 ccatcagcca ctcacctaac tgctgcgcct gcttatcatc agggtgattt gctaccgttc
    22621 ccgccagcgc ttcagtacgc agcgctttgt cacgccgccg ccataaccgt tccggtgaag
    22681 agccaagaaa agcattttcg ccatcaaagg ccatgtaaaa atggtagcaa ttcagattca
    22741 gtcgacgact ggcagccatc atcgccgccg cgttgaccgg acttgcgaaa tgcaggtcag
    22801 ttgcccgagc gagcaccact ttgtcgagct caccttcggc gatggttttc gttgccagtt
    22861 cgattaattg cgtccagccc gttttgtccg gccagtgttg ttctcgcgtg gtggttaaat
    22921 gtaacccagg caagggcttg atactcacca gtgtggcgat aaattctctt gccttaatcg
    22981 catcgtgctg aagggagctt tcgctgaata gcgtcagccg cagcgtggct ttaccgccac
    23041 agcgtcgcca ttccaggcgt ggtaaaagta aattgccctg cgacgggtca aacgcattca
    23101 gcccccaaat gcgtaagtcg gcgtgttccg ggtgctggcg aagaaaacgt tgtgcctggt
    23161 ccaacgacgt aaaacgggta atcgcgccca ggacggcagc ttcttcatca ccattacgtt
    23221 gttgccagta gaattgcggg tatgtttgct gactggccag ccagctcaag gcatcaaaag
    23281 cgtctttgag agggaaagga atatcgataa cccgaatgcc gggtgtcgcc ggaatctctt
    23341 gcgacaaatg gcgcagtaga ttttccagcg ccgtagtaag tgattgcacg cggacctctc
    23401 cctgttaaaa gcctcacatt atacggggta ctacaaaaaa aatgcagtac cccggtgtag
    23461 ggaggtttta acggcgtgcc agcaacagtc ctagtaccag cccaacggcc gcgcccacac
    23521 caattccttg ccagggtttt tcgtggacgt agtcatcagc acgataaact gcctgcttcg
    23581 cccgatagta ataactgtct gaagcctggc taacccgttt tttcacatca tccagcgctt
    23641 tttctgcacg cgctttcagc tcaacatatt tctgatcggc gggatcgcca gaggagcgga
    23701 gcacctcttc cagtgtttca ctaagcagcg tcaggtcgtc atcgatacgt gtatcaccaa
    23761 actgattaga cattctcgtt ctccatttgc gtaaaacctg tcactaacta tagacaaggg
    23821 ttgttgatta cgcctgaatt acctcgcgtg ccatcccaat gtgtgggata ccatcttctt
    23881 catagacctc cgtcacaggg ataaaaccga aactctggta gaagttttgc agatgcgcct
    23941 gcgccccgag gtaaacaggc ttatcaggcc agtgatgcgt gcaggtgtcg agggttttac
    24001 tcatcagttg ctgccccact ttttcgccac gcaacgcttc actgacaatc acccgaccaa
    24061 taacgaccgg ctcaagatca tcatcacttt tcagaatcct cgcatatgcc accagttcat
    24121 catttttcca accgagaata tggcgattat cccccgtcaa atcatcacca tcgatatcct
    24181 gataagggca attctgctcg acaacaaaca ccgcgcaacg cagttgtaat aaggcataaa
    24241 gctgggaaac cgataattca gagtgatgca gatcttacca ttcaatcata atgctctcct
    24301 tttagatgta tgccttatac tagccggaat attttttgaa acgtgatgaa ctcatggaat
    24361 taattttttt aggtacttca gccggtgtgc caacccgcac gcgcaatgtc acggcaatat
    24421 tgcttaacct gcaacatccg acccagagcg gactttggtt gtttgactgc ggtgaaggca
    24481 cccagcatca gctactgcat accgccttta accctgggaa actggacaag atttttatca
    24541 gtcaccttca tggcgatcat ctctttggtt tacccggctt gctgtgcagt cgttctatgt
    24601 caggcattat ccaaccctta acgatttatg gtccgcaagg tatccgtgaa tttgtggaaa
    24661 ccgcgctgcg gattagcggc tcatggaccg attatccgct ggaaattgtc gaaattggcg
    24721 ctggcgaaat tctcgatgat ggcctgcgca aagtgaccgc ttatccgatg gaacacccac
    24781 tggaatgtta tggctatcgt attgaagaac atgataaacc gggtgcatta aatgcccagg
    24841 cattaaaagc tgctggcgtg ccgcctggcc cactgtttca ggaattaaaa gcgggcaaaa
    24901 caatcacgct ggaagatgga aggcagatta acggcgcaga ttacttagct gctccagtgc
    24961 caggtaaagc gctcgctatt ttcggcgata ccggcccctg cgatgccgca cttgacctgg
    25021 ctaaaggtgt cgatgtcatg gtgcacgaag cgacgctgga tataaccatg gaagccaaag
    25081 ccaatagtcg cggccatagc tctacacgcc aggctgcgac actagcccgt gaggctggag
    25141 tcggcaagct aatcattacc cacgtcagct cgcgctatga tgacaaaggt tgtcaacacc
    25201 tgttacgtga atgcaggtca attttcccgg cgactgaact ggcgaatgat ttcaccgtgt
    25261 ttaacgttta act
//
LOCUS       DAEEKQ010000048        25178 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.048, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000048 DAEEKQ010000000
VERSION     DAEEKQ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25178
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.048"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..1092
                     /gene="dacC"
                     /locus_tag="KLK57_001867"
                     /pseudo
     CDS             1..1092
                     /gene="dacC"
                     /locus_tag="KLK57_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
     gene            complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="KLK57_001868"
     CDS             complement(1139..1897)
                     /gene="deoR"
                     /locus_tag="KLK57_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HBE0892246.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFNPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="KLK57_001869"
     CDS             complement(1955..2551)
                     /gene="ybjG"
                     /locus_tag="KLK57_001869"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBE0892247.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            2836..4063
                     /gene="mdfA"
                     /locus_tag="KLK57_001870"
                     /pseudo
     CDS             2836..4063
                     /gene="mdfA"
                     /locus_tag="KLK57_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
     gene            complement(4104..4388)
                     /locus_tag="KLK57_001871"
     CDS             complement(4104..4388)
                     /locus_tag="KLK57_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892248.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYARHLASVGEGMVIEHQIDDHHH"
     gene            complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="KLK57_001872"
     CDS             complement(4474..5289)
                     /gene="ybjI"
                     /locus_tag="KLK57_001872"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HBE0892249.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLLSFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(5289..6497)
                     /locus_tag="KLK57_001873"
     CDS             complement(5289..6497)
                     /locus_tag="KLK57_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0892250.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTRTAMENS"
     gene            6581..7117
                     /gene="rcdA"
                     /locus_tag="KLK57_001874"
     CDS             6581..7117
                     /gene="rcdA"
                     /locus_tag="KLK57_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HBE0892251.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(7292..8977)
                     /locus_tag="KLK57_001875"
     CDS             complement(7292..8977)
                     /locus_tag="KLK57_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HBE0892252.1"
                     /translation="MNINVAELLNGNYILLLFVVLTLGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            9247..9624
                     /locus_tag="KLK57_001876"
     CDS             9247..9624
                     /locus_tag="KLK57_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HBE0892253.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="KLK57_001877"
     CDS             complement(9654..9911)
                     /gene="grxA"
                     /locus_tag="KLK57_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HBE0892254.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            10071..10358
                     /locus_tag="KLK57_001878"
     CDS             10071..10358
                     /locus_tag="KLK57_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HBE0892255.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            10342..10807
                     /gene="nfsA"
                     /locus_tag="KLK57_001879"
                     /pseudo
     CDS             10342..10807
                     /gene="nfsA"
                     /locus_tag="KLK57_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
     gene            10868..11770
                     /gene="rimK"
                     /locus_tag="KLK57_001880"
     CDS             10868..11770
                     /gene="rimK"
                     /locus_tag="KLK57_001880"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HBE0892256.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11858..12334
                     /locus_tag="KLK57_001881"
     CDS             11858..12334
                     /locus_tag="KLK57_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HBE0892257.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12685..13797
                     /gene="potF"
                     /locus_tag="KLK57_001882"
     CDS             12685..13797
                     /gene="potF"
                     /locus_tag="KLK57_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HBE0892258.1"
                     /translation="MTALNKKWLSGLAAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13892..15025
                     /gene="potG"
                     /locus_tag="KLK57_001883"
     CDS             13892..15025
                     /gene="potG"
                     /locus_tag="KLK57_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HBE0892259.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIASRVHEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            15035..15988
                     /gene="potH"
                     /locus_tag="KLK57_001884"
     CDS             15035..15988
                     /gene="potH"
                     /locus_tag="KLK57_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HBE0892260.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALLYIWLILLF
                     LLPFLIVFKISLGEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGCPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15985..16830
                     /gene="potI"
                     /locus_tag="KLK57_001885"
     CDS             15985..16830
                     /gene="potI"
                     /locus_tag="KLK57_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HBE0892261.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDGITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            16890..17378
                     /locus_tag="KLK57_001886"
     CDS             16890..17378
                     /locus_tag="KLK57_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HBE0892262.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            17419..18546
                     /gene="rlmC"
                     /locus_tag="KLK57_001887"
     CDS             17419..18546
                     /gene="rlmC"
                     /locus_tag="KLK57_001887"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HBE0892263.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGDVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVMTLLVKQ"
     gene            complement(18721..19452)
                     /gene="artJ"
                     /locus_tag="KLK57_001888"
     CDS             complement(18721..19452)
                     /gene="artJ"
                     /locus_tag="KLK57_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HBE0892264.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDSVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19744..20412)
                     /gene="artM"
                     /locus_tag="KLK57_001889"
     CDS             complement(19744..20412)
                     /gene="artM"
                     /locus_tag="KLK57_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HBE0892265.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLALLLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(20412..21128)
                     /gene="artQ"
                     /locus_tag="KLK57_001890"
     CDS             complement(20412..21128)
                     /gene="artQ"
                     /locus_tag="KLK57_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HBE0892266.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(21135..21866)
                     /gene="artJ"
                     /locus_tag="KLK57_001891"
     CDS             complement(21135..21866)
                     /gene="artJ"
                     /locus_tag="KLK57_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0892267.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21884..22612)
                     /gene="artP"
                     /locus_tag="KLK57_001892"
     CDS             complement(21884..22612)
                     /gene="artP"
                     /locus_tag="KLK57_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HBE0892268.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22830..23345)
                     /locus_tag="KLK57_001893"
     CDS             complement(22830..23345)
                     /locus_tag="KLK57_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0892269.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATLLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23994..>25178
                     /locus_tag="KLK57_001894"
     CDS             23994..>25178
                     /locus_tag="KLK57_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBE0892270.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNSSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENMPSANYQFNTFFSNAFETDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQDQLDKHFTIESHPLLYRHT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKGAFIHFYDYSRRYAISENIEKDAVTNNIA
                     LMNTFYDSDNTSGEVLNNDFTEEEPFETALDVEHTIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMRSSHYHDICSVCVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGFFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLG"
BASE COUNT         6307 a         6053 c         6453 g         6365 t
ORIGIN      
        1 atgcgcgtgc atggatttta atggattacg ccagcggtaa agtgctggca gaaggcaacg
       61 cggatgagaa actggatccc gcgagcctga ctaaaatcat gaccagctat gtggttgggc
      121 aggcgcttaa ggccgataag attaaactca ccgatatggt gacggtcggt aaagatgcct
      181 gggcgacggg aaatccggca ctgcgtggtt catcggtaat gttcctcaaa ccgggcgatc
      241 aggtttcggt ggcagacttg aacaaaggtg tgattatcca gtccggtaat gacgcctgta
      301 ttgcgctggc cgattacgtt gccgggagcc aggagtcatt tattggtttg atgaatggtt
      361 atgccaaaaa aactgggtct gaccaacact accttccaga cggtgcacgg tctggatgcg
      421 ccggggcagt tcagtaccgc gcgcgatatg gcattgctgg gtaaagcatt gatccacgat
      481 gtgccggaag agtacgccat tcataaagag aaagagttca ccttcaacaa aattcgtcag
      541 cctaaccgta accgtctgct gtggagcagc aatctgaatg ttgatggcat gaagacagga
      601 accacggcag gtgcgggata taatctggtt gcttcggcta cccagggtga tatgcgttta
      661 atctccgtag tgctgggggc gaaaactgac cgtatccgtt ttaatgagtc tgagaaatta
      721 ttgacctggg gtttccgctt ctttgaaact gtgacgccaa ttaaacctga tgccaccttt
      781 gtgactcagc gcgtctggtt tggtgataag agcgaagtga atctcggggc aggcgaagcg
      841 ggctccgtga ccataccgcg tgggcagctg aaaaacctga aggcgagcta cacgttaacg
      901 gaaccgcagc ttaccgcacc gctgaaaaaa ggtcaggttg tcgggaccat tgatttccag
      961 cttaacggta aatccattga gcagcgtccg ctgatcgtga tggaaaatgt ggaagagggc
     1021 ggattctttg gtcggatgtg ggatttcgtg atgatgaaat tccatcagtg gttcggcagc
     1081 tggttctctt aatcttctga taaccggatg gcgcgaaacg tcatccggtt atacgtcatt
     1141 aatacatcaa cttaatacgg tgcgtctgcg cgtacttcac atactcatct tccgggcaac
     1201 aatcactcac cacaatatca aagcgtttca ggtcacccat gcgcgccgga cgcaccttgc
     1261 caaatttact gtggtcgaca accagcacat gcttttgcgc catcgacatg gcccagtgtt
     1321 ttaccggcaa ctcttcaaga ttaaaacagg tagcgccttt actgacatgc acgcccgccg
     1381 cggaataaaa agcgatatcc gggcaaaaat tattcaacgt ttgctgaaaa tcgatggggt
     1441 tgaaaatggc gttgctggcg tgaaattcac caccgcacag aaatgcgcgg caatggggtt
     1501 tctctttcag cgccagaaag gtatttagcg aataacaaac ggcggtaaaa gggatttcat
     1561 tatcaatcgc ttcaataatc cacggcgtgg tggtgccaca gtcaaaaaag agggtctgat
     1621 cgggttctac cagtgtcgca gccagttttg ccgcccggcg tttttcttcc accaggcggg
     1681 atttttgatc gcttaacagg taatggctgg cactgcgcgg ttccagaaca atatagccgc
     1741 cgagcaaaac gacgggcgca ctgtggttgt tcagatcgcg acgaatcgtc atctccgaaa
     1801 ccccaagcag ggcggcggcg tctttaagat gtaacttatc gctgcgtttt aattcttgca
     1861 gcagctgccc gatacgctct tcgcgacgtg tttccataat ccctctgaat agttattgaa
     1921 gcgagccgct caatactaca ctttttagca gagatcagtc acgcacccag cctttgcgga
     1981 tcggtaatgc aaaacagacg cgataccacg attgcagacg ttgatagagt ttatgcccca
     2041 tcgcttgcca gataatctgg gcactaaggc agccaatcat acctgccagc aatccaccga
     2101 gcatatccag cggccagtgg acgccaagat aaacgcgcga ccaggcaatg acgacggcca
     2161 gaaccattaa aagtgagccg gaccacaggc gatgccagca taaaaatgcc agtgcaaagg
     2221 tgaaaatcac cgtaccgtga tcgctcggga atgagtcatc cgccgcatga tgcaggaagt
     2281 tatagccgat attttcgaca aagggtcggt cgtgcggaaa aagatgtccc atcgtccagg
     2341 acacaaacag gctgaccgcc agcgcgatag cgatttttat caccagttgc cgttgtgctg
     2401 taagccccca caaccaaagt accgcggcca gcaacggcac cacggtaatc aaatctttag
     2461 caataaaaat cgccaacgag atcatccacg gagccgagtc tggcgtcgcg ttaataagag
     2521 agaatagaga gagattcaaa ttttccagca taacttcccg acgcaaagtg attaaaaggg
     2581 agccaataca ggcaagtcgt tgagaataaa gtgcaggtta aactgggtaa agcggcatcg
     2641 tcttatttcc ctcaagcggc ctgtttacgg tgggtgattg taacgggcat aggttaaata
     2701 aaacttaaag aaagcgtagc tatactcgca ataatgtaag aatgtgctta accgtggttt
     2761 cagctacaaa attcgctttc tcgttagctg cgcttttatt aaactctgcg cgattattat
     2821 tggcgaagaa attgcatgca aaataaatta gctttcggtg ccaggcttgg acgtcaggcg
     2881 ttacttttcc ctctctgtct ggtgctttac gaattttcaa cctatatcgg caacgatatg
     2941 atccaacccg gtatgttggc cgtggtggaa caatatcagg cgggcattga ttgggttcct
     3001 acttcgatga ccgcgtatct ggcgggcggg atgtttttac aatggctcct ggggccgctg
     3061 tcggatcgta ttggtcgccg tccggtgatg ctggcgggtg tggtgtggtt tatcgtcacc
     3121 tgtctggcaa tattactggc gcaaaacatt gaacaattca ccctgttgcg cttcttgcag
     3181 ggcataagcc tctgtttcat tggcgctgtg ggatacgccg caattcagga atccttcgaa
     3241 gaggcggttt gtatcaagat caccgcgctg atggcgaacg tggcgctgat tgctccgcta
     3301 cttggtccgc tggtgggcgc ggcgtggatc catgtgctgc cctgggaggg gatgtttgtt
     3361 ttgtttgccg cattggcagc gatctccttt ttcggtctgc aacgagctat gcctgaaacc
     3421 gccacgcgta taggcgagaa actgtcactg aaagaactcg gtcgtgacta taagctggtg
     3481 ctgaagaacg gccgctttgt ggcgggggcg ctggcgctgg gattcgttag cctgccattg
     3541 ctggcgtgga tcgcccagtc gccgattatc atcattaccg gggagcagtt gagcagctat
     3601 gaatatggct tgctgcaagt gcctattttc ggggcgttaa ttgcgggtaa cttgctgtta
     3661 gcgcgtctga cctcgcgccg caccgtacgt gataattatg ggcggctggc cgattatgat
     3721 tggtctgttg gtcgctgctg cggcaacggt tatctcatcg cacgcgtatt tatggatgac
     3781 tgccgggtta agtatttatg ctttcggtat tggtctggcg aatgcgggac tggtgcgatt
     3841 aaccctgttt gccagcgata tgagtaaagg tacggtttct gcggcgatgg gaatgctgca
     3901 aatgctgatc tttaccgttg gtattgaaat cagcaaacat gcctggctga acgggggcaa
     3961 cggacagttt aatctcttca accttgtcaa cggaattttg tggctgtcgc tgatggttat
     4021 ctttttaaaa gataaacaga tgggaaattc tcacgaaggg taaaaaaatg cctgactgct
     4081 ttgtgcgatc aggcattctc gaattaatgg tgatggtcgt caatctggtg ttcgataacc
     4141 atcccttcac ctacgctggc aagatggcga gcataaggat gcgggcggta agccggagct
     4201 ggcgcaggag ccacatagac ggtttgcggc acagtcgcga cgctgaccgc ttgtggaacg
     4261 ctgactgaat caggaactac aaccactttg tatccactcg gcacatcgac ggtgacactt
     4321 tgcgccatcg ccacaccagt aaagcccatt aaaagcgcgc ccaacagtag acaatttttc
     4381 ataatcattt cgcctgaaat aatgcagcgg gttaatttcc gcgaattatg cagccttcat
     4441 tcaggtattg tgttgccggg cgtgctgccg tttttattgg tcaaatggcg cttcgtgttt
     4501 aagcacttta tcgatcacat ccagtacgcc ttcacggtta ttggagcctg cccggtattt
     4561 tgccgctgcg acgaccgcgc tgccggcatt ttccattgca aaactaaagc ctgcctgacg
     4621 cagcatctca atatcgttac cgccatcgcc aaagaccacc acttcgctgt cgtctattcc
     4681 ccataatttc tgcagttggc gaaggccatt ggctttatgt acgccgggga taatcagatc
     4741 gatgctgccg ttgccggtgt ggactggcac cataatatcg ccgatggcct catgtaatgc
     4801 tttttgtact tgtggaatca gttcatcgga aagattcagg ccaaacttaa agaaaatatc
     4861 ttctaagttg tcaaagttat cgacgtattc cagacggtga taatacattt ctgccaccgt
     4921 tttcatggca tcgtcatatt ttttgagcgt ataggcgcta ttttttccgc aggcaataat
     4981 ttccacttcc gggcgcgtca gcaaatgttc cacgacagta gtaaaggcat ccttcgacag
     5041 ctcgccatta aaaacatctt tgccttcact cactacccag ccgccgtttt cagccacaaa
     5101 ggcaatttca ttagcaattt cagggaagaa agagagcaac tgataatatt gattcccgct
     5161 ggcgaccaca aagcgaatgc cttgtgcttt catttgctga tactgagcca taaaccgctc
     5221 acggttatag gttttttgat cgcttaagaa agtaccatcc atgtctaccg caattaattt
     5281 aatgctcatc aactattctc catcgccgtc cgcgttttgg tatcgggttt ggcgacggct
     5341 ttcgcgacaa tagccgcgag aataaccagc gccagtacaa ccagcattgc actacgtaat
     5401 ccataatgtt cgccgagata gcccagcagc ggcggcccga cgaggaaagc cagataaccg
     5461 gtcgttgcta ccacactgac gcgggttggt gcatcggggc cggtatcgct ggcggcagaa
     5521 atggtcagcg ggaagcccag cgaggcaccc agtccccaga gtacaacaga caccccagcg
     5581 acccaggcgc tatcgacaaa aataatcagc ccaataccca acgcccccat tagtgcactg
     5641 gcccgaacca cggcaacgcg actgtaacgg tcgatgaacc aaccgccagt gaagcgtcca
     5701 acggtcatcc ctagggtaaa accggcataa atcagcgagc cggaagtagg gctaaaaccg
     5761 tgaccgtcaa ccattaataa gggtaaccag tcgttggcag aaccttcggc aaaggccatc
     5821 gccagcacta caacaccaat caacagcaac tggatatcgc gataaaaagg aatgcctttt
     5881 tcgccatgct gggtgccatc ggcagcattt ttgcccgtac cgtcagggat tgcctgaatg
     5941 gcgatataaa taggcgcgat acctaccagc gccgccaata aaatgtgcac cgttgccgga
     6001 acgccaaagg ccgtcagtgc catcccgaca ccagcgcctg ccagcgtgcc taggctataa
     6061 aaaccgtgca tcatcggcaa aaccgtttta ttcatttctc gctcaacggc ggcaccttca
     6121 acgtttatcg ccacttccgc agaaccaaaa cttgccccaa agacgccgag accaacggca
     6181 aagagcaggg gcgatgtcag ccagagtgcc agacttaata tcatcatccc gatcaatgcg
     6241 caggacatcg tgaccaggat gacattacgt gtcccaaagc gtttcactaa ccacgccgag
     6301 cagagaatac cgctcatcga accgatcgac agaccaaaga gaacaccgcc catttcagcg
     6361 atcgagacag agagaatatc gcggatagca ggcgtacggg ttgcccagga cgccattaac
     6421 aggcctggca aaaagaagaa cataaacagc gcccaggttc ggcgtttcaa tgcattacgt
     6481 gaagaattta cggtcataga tcacgtcaaa ataagaagag ggaagacaac attagcaagg
     6541 ttgtgtacat ttgtacacaa ttgcagacag aggaaatgac atgcgtcgcg ctaacgatcc
     6601 gcaacggcga gaaaaaatta tccaggccac gctggaggcg gtgaaacttt acggaataca
     6661 tgctgttacg caccgcaaaa ttgctaccct tgccggggta ccgttgggat cgatgaccta
     6721 ctatttttca ggaattgatg agttgttgct ggaggcgttc agcagtttta ctgagattat
     6781 gtcccggcaa tatcaggcat tttttagcga tgttagtgat gctcaaggcg catgccaggc
     6841 tatcaccgat atgatctaca gctcacaggt tgcaacgccg gataatatgg agctgatgta
     6901 ccagctctac gcgctggcta gccgaaaacc gctattaaaa acggtaatgc aaaactggat
     6961 gcagcgcagt cagcaaacgc tcgaacaatg gtttgaaccc ggaaccgccc gcgcgcttga
     7021 tgcgtttatt gaggggatga cgctgcattt tgtcaccgac cgtaagccgc tatcgcgcga
     7081 ggagattttg aggatggttg agagggttgc agggtagtag ataaatttta gataacaaaa
     7141 aacccatcaa ccttgaaccg aaatggcggg gttgatgggc tccacaaaat ggggacatca
     7201 aagaaaagca gtggcactaa ttaagactga tgccctgcgg aaaagttctg cggttgtgca
     7261 aaaaaatttc attttcaggg caacttcagt tttatcctaa tcctggccat accatgacga
     7321 tgattgtccc tgccagcgtc agcaggacgt tggcgattgc gtaggtgccc gcatagccca
     7381 gcgcagggat gttactgcga gctgtatcac tgatgatctc cattgccggc gcgcaggtgc
     7441 gtgcgcccat cattgcgccg aacaacagtg cgcggttcat tcgcaataca taagcaccga
     7501 acaagaaaca gataaccacg ggcacaagac tgacaattaa tccggcaatc aacatctgac
     7561 cgccaatcgc gcccaggccg ttattaatac cgctaccggc gctcagacca acgcctgcca
     7621 taaacaccat caagccgaac tctttcacca tgcttaatgc accttgcgga atgtaaccga
     7681 aggtcgggtg gttagcacgc ataaagccca gcataattcc ggcgaataac aacccggcag
     7741 cgttccccat gccgaaactg aatgtgctga actggaaggt gatcatcccg atcatcagcc
     7801 caataacaaa gaaggcgcaa aatgccagca ggtcagtgac ctggctgtga atcgagataa
     7861 agccgatgcg atcggcgatg gtttttacgc gacgggcatc accgctgact tgtaaaacgt
     7921 cacctttgtt aagcacgacg ttgtcatcta tcggcatctc aatctggcta cgaatgacgc
     7981 ggttaaggaa gcaaccgtga tcggtcaact tcagttgtgc gagacgttta cctacagcgt
     8041 tatggttttt aacgaccact tcttcagtga cgatacgcat gtcgagaagg tcacgatcga
     8101 aaacttcttt accgttacgg aagctgggat cgagtcgggc atgggcgtcg ggatagccta
     8161 ccaacgctat ttcatcgccc atttgtagca cggcatcacc gtctggattt gccagaatcc
     8221 cgttacgtcg aatacgttca atgtagcagc cggtttgtcg ataaataccc agttcacgca
     8281 gatttttgcc gtcggtccag gccaccagct ccgggccgac gcgataggcg cggatcaccg
     8341 gtaaataaac cttacggttg gcatcagtgt ccaggccacg ttcgcgggcg atttgctggg
     8401 cgctggtctg taagtcctga tgctgcaatt tcggcaagta acgcgcacca acaatcaaac
     8461 tcaccagacc gattaaatag gttaaggcat acccgaggct cagattatcc agtgccagtg
     8521 agagctgcct gctttccatg ccggaatgac gcagtgtatc gccagcaccg accagaaccg
     8581 gtgtcgacgt catagagcct gctaacatac cggccgtcag gccaatatcc cagccaaaca
     8641 gcttacctaa ccctaaggcg atcaccagcg cactgccaac catcaccagt gctaacatta
     8701 ggtaattttt cccatcgcga aaaaaaatgg aaaaaaagtt cggtccggct tcgaccccga
     8761 cgcagaaaat aaacagcata aagccaagat taagcgcatc ggtgttaatg ctgaaatgtt
     8821 gttggcctaa taacagcgat acgactaaaa cgccaatgga attacccagt tggatcgaac
     8881 caagtcgtaa ctttccgaga catagcccaa gcgtgaggac cacaaataat aacagaatgt
     8941 aattcccatt taacaattcg gcgacgttta tattcacgga ggctaacttc ttgtttacta
     9001 gtaagctgtt gaaagaaatg gtaatttacg ataatgtttt ttaccagaat tcagggcgca
     9061 gattcattca gcgcacctaa acgatagtaa agtaacaata tattttacta gtgtaatcac
     9121 attaggtatc aacggctata tgaattgcgt tggcctatat tagcatggaa tgcgaagcgg
     9181 ctttatctta ctgaacgcca cactggcgaa aaatgtgttc gatagacgca gtgtcaggag
     9241 gaacgagtga aacataaaca acgttgggcg ggggcaatct gctgttttgt cctcttcatt
     9301 gtggtgtgcc tttttctggc gacgcacatg aaaggcgctt ttcgggctgc cgggcatcct
     9361 gaaatcggct tgctgttttt cattcttcct ggagcagttg ccagtttctt ttcacagcgt
     9421 agagaagtcc tgaaacctct atttggcgca atgctggcgg caccctgttc aatgctcatt
     9481 atgcggctgt ttttttcacc gacgcgctca ttctggcaag agctggcatg gttactaagc
     9541 gcggtgttct ggtgtgcgct gggggcactg tgtttcttat ttatcagtag tttgtttaaa
     9601 ccacagcaca gaaaaaatca gtaaagccct caacgcgagg gcttgtcaga cgatcaggcg
     9661 tccagatttt ctttcaccca tgcagcaaaa tcggtatagc cgccgatatg ttgctgatcg
     9721 acaaaaatct gcggcacggt ttctacgggt ttacctgcct tttgttgtag atcttcttta
     9781 gtgatccctt ccgcacgaat atctacatac tgatactgaa aatcatcgcg ttcattgctc
     9841 aatttctcag ccagatcttt tgcacgcaca cagtaagggc aacccgaacg accaaaaata
     9901 acggtttgca ttatttctct cctcatagat ttatgcctgt aatgatcacg ctaaaatgta
     9961 ttcgctgaaa gtaggtttaa cctgttgcat taattgctaa aagctataac tgttaaacac
    10021 aatacagtga aaagttttag actgaaggct cactttgcag agggaagcgt atgcgcgcga
    10081 tcggtaaatt gcctaaaggc gtgttgatac tggaatttat cggaatgatg ctactggcgg
    10141 tggcgctgct gtcggtaagc gactccctgt cgctgcctga gccattttct cggccagaag
    10201 tgcagattct gatgattttt ctcggtgttt tgctcatgct tcccgctgcg gtggtggtta
    10261 ttcttcaggt ggcaaaacgt cttgccccac agctgatgaa ccgtccaccg caatattcac
    10321 gttcagaaag agaaaaagat aatgacgcca accattgaac ttatttgtgg ccatcgctcc
    10381 attcgccatt tcactgatga acccatttcc gaagcgcagt gtgaggcgat tattaacagc
    10441 gcccgtgcga cgtccagttc cagttttttg cagtgtagta gcattattcg cattaccgac
    10501 aaagcattac gtgaagaact ggtgacgctg accggcgggc aaaaacacgt agcgcaagcg
    10561 gcggagttct gggtgttctg tgccgacttt aaccgccatt tacagatctg tccggatgct
    10621 cagctcggcc tggcggaaca actgttgctc ggtgtcgttg atacggcaat gatggcgcag
    10681 aatgcattaa ccgcagcgga atcgctggga ttgggcgggg tatatatcgg cggcctgcgc
    10741 aataattaaa gaaagccgcc catttattct ggattatttg cacaaacagg gttgggcgac
    10801 gcgctaaaac cgccacgtcg atgtatgata cgcgggcttt tgaccaggtc tgacagagag
    10861 gtgcagggtg aaaattgcca tattgtcccg ggatggaacg ctctattcgt gtaagcggct
    10921 gcgtgaagcc gctatacagc gcggtcacct ggttgaaatt cttgatccgc tttcttgcta
    10981 catgaacata aatcctgcgg cgtcttctat tcactacaaa ggccgcaagt taccccattt
    11041 tgacgcagtg atcccgcgta ttggcaccgc cattaccttt tatgggacgg cggcactgcg
    11101 ccagttcgag atgctgggga gctatccgct caatgagtcg gtcgccattg cccgggcgcg
    11161 tgacaaattg cgttccatgc aactgctggc gcgtcagggc atcgacctgc ctgtcacggg
    11221 cattgcgcat tcgccggatg ataccagcga tttaatcgac atggtcggtg gtgcgccgct
    11281 ggtggtcaag ttggttgaag gcacgcaggg aattggcgtc gtgctggcgg agacgcgtca
    11341 ggcggcggaa agcgtgattg acgctttccg cggtctgaac gcgcatattc tggtgcagga
    11401 atatatcaaa gaggcgcaag ggtgcgatat ccgctgtctg gttgttggcg atgaagtggt
    11461 cgctgcgatt gaacggcggg cgaaagaggg cgattttcgt tccaatttgc atcgtggcgg
    11521 cgcggcaagc gtcgccagta tcacaccaca ggagcgtgaa atcgcgataa aagccgcgcg
    11581 aacgatggcg ctggacgttg ctggtgtgga tattctgcgt gctaatcgcg ggccgttggt
    11641 gatggaagtg aatgcgtcgc cggggctgga aggaatagaa aaaaccactg gtatcgacat
    11701 cgcgggtaaa atgatccgct ggatcgaacg ccacgctacg acagaatatt gcctgaaaac
    11761 gggtggttag tcgcaatcac attactgatc atggttttgc ctgcgctttt tgcgtaagct
    11821 gtgccggtct ttttatcgaa agaggttgta caaaattatg acatcgctgg tcgttcctgg
    11881 tctggatacg ctgcgtcaat ggctcgatga cctggggatg agtttttttg aatgtgataa
    11941 ctgtcaggct ctgcatctgc cccatatgca gaatttcgac ggtgtctttg atgccaaaat
    12001 cgatctgatc gataacacga tcctgttttc tgccatggcg gaagtccgac cttcagccgt
    12061 attgccgctg gcggcggatt tatctgccat caatgccagt tcgctgaccg tgaaagcatt
    12121 tcttgatatg caggatgata atctgccaaa gctggtggtt tgccagtctt tatccgttat
    12181 gcagggcgta acctatgagc agtttgcatg gttcgtgcgt cagagcgaag agcagatttc
    12241 gatggtcatt cttgaagcta atgcccatca actgctgtta ccgactgacg atgaagggca
    12301 aaacaacgtt accgaaaact atttcctcca ctgataactc ctttcgagca cgcagtcgct
    12361 ggtgcagtgg ctgcgcgctg caaaattatc tgctgttttt aaccttttct taaagattat
    12421 ttcacttctc ttgtgtcgat ttggctttat cacatagagc aaatacgcat aaaaatttgt
    12481 taaataccgt tttttaatcc gagctatagt ctcaaaccct ggctaaagtt attcttgcga
    12541 tgcttttata tagcgagcag tgctggccgg gagagagttc tcttttctta caccccgccg
    12601 ataaaaaata tgcacgttta ttgcatatct tttagtgtga caacttttgt tcgtttgtta
    12661 acgaactttc agaaggaaag agatatgacc gccttaaata aaaaatggct ttcgggtctg
    12721 gctgcgggtg ctctgatggc cgtctctgtc ggcacgctcg cggctgaaca aaaaacactc
    12781 cacatttata actggtctga ttatatcgcc ccggacacgg tggccaattt tgaaaaagaa
    12841 accggtatta aagtcgtcta cgatgttttc gactctaacg aagtactgga aggcaaatta
    12901 atggcgggta gcaccggctt tgacttggtg gttccatctg ccagctttct tgaacgccag
    12961 ttgactgcgg gagttttcca gccgctggac aaaagcaaat tgccggagtg gaagaatctc
    13021 gacccggagc tgctgaagct ggtcgccaaa cacgatcccg acaataaatt tgctatgccc
    13081 tatatgtggg cgacgaccgg gattggctat aacgttgata aagttaaagc tgtgctgggc
    13141 gaaaacgcgc ccgtcgatag ctgggacttg atcctcaaac ctgaaaatct ggaaaaactg
    13201 aaaagctgcg gtgtctcttt cctggatgcg ccagaagaag tttttgctac cgtgttgaat
    13261 tatctcggca aagatcccaa cagcactaaa gcggatgatt acaccggacc ggcaaccgat
    13321 ctgctgttaa agctgcgccc gaacattcgt tatttccatt catctcaata cattaacgac
    13381 ctggcaaacg gcgatatttg cgtcgctatc ggctgggcag gtgatgtctg gcaggcgtca
    13441 aaccgcgcga aggaagcgaa gaatggagtg aatgtctcgt tctcgattcc aaaagaaggg
    13501 gcgatggcgt tctttgatgt attcgccatg cctgcggatg ccaaaaacaa agacgaagcc
    13561 tatcagttcc tgaattacct gctgcgcccg gatgtagtag cgcatatttc cgaccatgtg
    13621 ttctatgcca acgccaataa agcagccacg ccgctggtga gtgcggaagt ccgtgagaac
    13681 ccgggtattt atcctccggc ggacgttcgt gcgaagctat tcactctgaa agtacaggat
    13741 ccgaaaatcg accgtgtgcg cacccgcgcg tggaccaaag tgaagagcgg aaaataatcc
    13801 gcagccttag atgccgggcg ggcgcaccac accagccggc aattcgcacc atcatggtgc
    13861 gcttgcacac attcaatgcc ggagagcagc cgtgaatgac gctatccctc gcccgcaggc
    13921 gaaaacccgt aaggcgctga cgccgctatt agaaatccgc aacctcacca aatcctacga
    13981 tggtcagcat gcggtcgatg atgtcagcct gaccatctac aaaggtgaaa tcttcgcgct
    14041 gctgggcgca tccggctgtg gcaagtccac gctgctgcgt atgctggcag gttttgaaca
    14101 accttctgcc gggcagataa tgctcgatgg cgtcgatttg tcacaggtgc cgccttacct
    14161 gcgccccatc aatatgatgt ttcagtctta cgcgctgttt ccgcatatga ccgtggaaca
    14221 gaacatcgct tttggcctga aacaggacaa actaccgaaa gcggaaattg ccagtcgggt
    14281 ccatgagatg ctcggcctgg tgcacatgca ggagttcgcc aaacgcaaac cgcatcagct
    14341 ttccggtggt cagcgacagc gtgtggccct ggcccgaagc cttgccaaac gcccgaaact
    14401 attactgctc gatgagccga tgggcgcgct ggataaaaag ctgcgtgacc ggatgcagct
    14461 tgaagtggtg gatattctgg agcgcgtcgg tgtgacttgt gtgatggtca cccacgatca
    14521 ggaagaggcg atgaccatgg cggggcgtat cgccattatg aatcgcggga aatttgtcca
    14581 gattggcgag ccggaagaga tctacgagca tccaactacc cgctacagcg ctgaatttat
    14641 cggttcggtt aatgtctttg aaggtgtact caaagagcgt caggaagatg gcctggtgct
    14701 tgattcgccg gggctggtgc atccactgaa agtcgatgcg gatgcttcag tggtcgataa
    14761 cgtgccggta cacgtagcgc tgcgcccgga aaaaatcatg ctttgcgaag agccgcccgc
    14821 caatggttgt aacttcgcgg tgggggaggt gatacacatt gcctatctcg gcgatctttc
    14881 ggtgtatcac gttcgactga aaagtgggca gatgatcagc gcccagctac aaaacgccca
    14941 tcgtcatcgt aaagggttac cgacctgggg cgacgaagtg cgtttgtgct gggaagtgga
    15001 cagctgtgtg gtgctgacgg tttaaggagc aaagatgagt acacttgaac ctgctgccca
    15061 gtcgaaaccg ccgggcggat ttaagctgtg gttgtcgcag ctgcaaatga agcatgggcg
    15121 caaactggtc attgcgttgc tatatatctg gttgatcttg ctgtttctgc tgccatttct
    15181 gattgtcttt aaaataagcc tgggagagat ggcgcgcgct attccacctt ataccgaact
    15241 gatggagtgg gctgacgggc aactttccat cactcttaat ctcggtaatt ttctgcaact
    15301 gaccgacgat ccgctctatt ttgatgctta tctccagtcg ttacaggtgg cggcgatttc
    15361 gacattttgc tgtttactga tcggctatcc gctggcgtgg gcggtggcgc acagtaagcc
    15421 ttcgacccgt aatattttat tactactggt gatcctgccg tcgtggacct cgtttctgat
    15481 ccgcgtttat gcctggatgg gaatattaaa aaacaacggt gtgctgaata attttctgct
    15541 gtggctgggg gttatcgatc aaccgctgac cattctgcat accaatctgg ccgtttatat
    15601 cggcattgtt tacgcttacg tgccgtttat ggtactgccg atttataccg cgttgattcg
    15661 tattgattat tcgctggtgg aagcagcgct ggatctcggt gcacgaccgc tgaaaacgtt
    15721 ctttactgtg atcgtgccgc tgactaaagg tgggattatt gccggatcga tgctggtgtt
    15781 tatcccggct gtgggcgagt ttgtgatccc ggaactgctc ggttgcccgg acagcatcat
    15841 gatcgggcgc gtgttatggc aagagttttt caataaccgc gactggccgg tggcctcggc
    15901 ggtagcgatc atcatgttgc tgctgctaat tgtgccgata atgtggttcc acaaacacca
    15961 gcaaaaaagc gtgggagaac acggatgaat aatttaccgg tagttcgttc gccctggcgg
    16021 attgtgattt tgctgctggg ctttaccttt ctctacgcgc caatgctgat gctggttatc
    16081 tattcgttta acagctcgaa gctggtgacg gtgtgggccg gctggtcaac gcgctggtat
    16141 ggtgagttat tgcgcgatga cgcgatgatg agtgcggttg gtttaagcct gacaattgct
    16201 gcctgtgcgg caacggcggc ggcgatcctc gggactattg cggcggtggt gctggtgcgt
    16261 tttggcaggt ttcgcggatc aaatggtttt gcctttatga tcaccgcgcc gctggtgatg
    16321 ccagatggca tcacgggctt gtcgctgttg ttattattcg tcgcgcttgc tcatgccatt
    16381 ggctggcctg cggaccgcgg tatgctcacc atctggctgg cgcatgtcac tttttgtacg
    16441 gcttatgtgg cggtcgttat ttcttcaaga ttgcgggaac tggatcgctc gatagaagaa
    16501 gcagcgatgg atctcggtgc gacgccgctg aaagtgtttt tcgtcattac gctaccgatg
    16561 atcatgcccg cgatcatttc tggctggtta ctggctttta ctttgtcgct tgatgatctg
    16621 gtgatcgcca gctttgtttc cgggccggga gccaccacgt taccgatgct ggtcttttcc
    16681 agcgtgcgga tgggagtgaa tccggaaatc aacgcccttg caacgttaat tctcggtgcg
    16741 gtcggaattg tcggatttat cgcctggtat ctgatggctc gcgcagaaaa acagcggata
    16801 cgcgatatcc agcgtgcaag acatggctga agacactaaa atttgccaac ctggctacat
    16861 aatgccgcgc atgccgcggc attgttttca tggaagacga aacgttggga ttttttaaga
    16921 aaacatcttc atctcatgct cgcctgaatg tgcctgcgct ggtgcaggtg gcggcgctcg
    16981 ccattattat gatccgtggc ctcgacgtgc tgatgatttt caatacgctg ggcgtgcgcg
    17041 gtattggcga gttcattcat cgcagcgtac aaacctggag tttaacgctg gtctttttaa
    17101 gcagtctggt gctggttttc attgagatct ggtgtgcgtt ttcactggtg aaagggcgtc
    17161 gctgggcgcg ctggctatat ctgctgacac aaatcaccgc cgcaagttac ttgtgggcgg
    17221 cttcgctggg gtacggttat ccggagctgt tcagcattcc cggtgaatca aaacgtgaaa
    17281 tcttccatag cctgatgctg cagaagctgc cggatatgct catcctgatg ctgctgttcg
    17341 ttccctcgac cagtcggcgg ttcttccagt tgcaataatg tgtataatcg tcgcccctga
    17401 tgatgtgaag gtcaatgtat gcagtgcgca ctttacgacg cgggtcgctg tcgttcctgt
    17461 cagtggataa cgcagccgat tccagagcaa ctctccgcta aaaccgccga tcttaaaaat
    17521 ctgctcgccg attttccggt tgaggaatgg tgcgcgccgg tgtcaggccc ggaacaaggg
    17581 tttcgtaata aagccaaaat ggtggtgagt ggtagcgttg aaaaaccact gctcggtatg
    17641 ctgcatcgcg atggcacgcc ggaagacctt tgtgactgcc cgctttatcc tgcctctttt
    17701 gcgcccgttt ttgcggcgct aaaaccgttt atcgcccgtg cggggttaac gccctacaac
    17761 gtggcgcgta agcgtggcga actgaaatac attctgctga ctgaaagcca gagcgatggc
    17821 ggcatgatgc tgcgctttgt actgcgttct gaaaccaaac tggcacaact gcgtaaggcg
    17881 ctgccgtggt tacaggaaca actaccgcaa ctgaaagtta ttaccgtcaa tattcagccg
    17941 gtacatatgg cgattatgga aggggagacg gagatctacc tgactgaaca acaggcgctg
    18001 gcagagcgtt ttaatgacgt accgctgtgg atccgtccgc aaagtttctt ccagactaat
    18061 ccggcggtcg ccagccagct gtacgctacc gcgcgcgact gggtacggca gctgccagtt
    18121 aaacatatgt gggatctgtt ctgcggtgtg ggtggctttg gtttacactg cgcgacgcct
    18181 gacatgcagt taaccgggat cgaaattgca tcagaggcca ttgcctgtgc aaagcagtca
    18241 gccgctgaac tgggcttaac gcgtttgcaa tttcaggcgc tggactccac tcagtttgcc
    18301 accgctcagg gggatgtgcc ggagctggtg ctggttaacc cgccgcgccg cggcattggt
    18361 aaaccgctgt gtgattatct ctcaacgatg gcaccgcgtt ttattatcta ctccagctgt
    18421 aacgcccaaa ccatggcgaa agatatccgc gaactgccag gttaccgcat cgaacgggta
    18481 cagctttttg atatgttccc gcacaccgcg cactatgaag tgatgacgct gctggtgaag
    18541 caataaaaaa gccgcaggtg cggcttcaga ttgctgatca ggtgcgcttt gttcatgccg
    18601 gatgcggcgt gtacgcctta tccggcctac aaaatcatgc taaatcaata cattgcagag
    18661 ttgatgtggg ccagataagc gcagcgcatc aggcaatctt acttttgtca tcagtctcaa
    18721 ttactgcggg aaccactggt cattgatttt ctgatatgta ccgtcagctt taattgctgc
    18781 cagcgcgtta ttcagttttt ccagcagagc tttgttatcc ggacgtacag cgatgcccag
    18841 accggtgccg aagtattgcg gatcggtcac tttttcagta gcaacgccca gttgcggatt
    18901 ggttttcagc cattcgttta ccaccgctgt gtcaccaaat accccatcaa tacgaccatt
    18961 tttcagatcg ataaaggcat tctgataact gtcataggag acagttttca cttcggggtg
    19021 ctgatcctga atatatttct ggtgcgtggt gccgttttcc atcccaatac gtttgccttt
    19081 cagatcggca aacgttttgt aggtatcttt tttggcaatc acgacggctg agttttcata
    19141 gtagggcgtg gtaaacgata cctgcttgct acgctcgggc gtgatatcca taccggagat
    19201 tacggagtca tattttctga atttcagtga cgggatcagg ctgtcgaacg cgtgattagt
    19261 aaaagtacat tctgcctgca tttgtttgca caaggctttt gccagatcga tatcaaagcc
    19321 gacaatctca ttattagcac ctatagattc aaagggtgga taggtggctg aaacgccaaa
    19381 attgattttc tctgcggcag aagcaccgaa agtaaaggaa gcaagtaaag cggcaagaac
    19441 taactttttc atgatggaac tcccgtctgt caatcttatg atttttggcc gtgtctgcgg
    19501 catgggataa caatgccatc aagtgaattt atatgcaata aatatgatta aataatttaa
    19561 atgaaataaa aaaagacgga taacttagtg agttgtccgt cttcattata agaatttatg
    19621 cactatgtag gccggataag gcgtccacgc cgcatccggc acaggcaccg tgctgatgtc
    19681 tgatgcgacg ctggcgcgtc ttatcagacc tacaaacccc cccggcgaat gtacgcagct
    19741 acattaattc cgccgttcga atgccagtgc tttgcgctcg atcagacgca tcatcagcgt
    19801 cagcaggccg ttgacgacca ggtaaataat ccctgccgca ccgaacacca ttacatcgta
    19861 ggtgcgtccg tacaacaact ggctgtatcc catcacttcc atcagcgtaa tggtgtaagc
    19921 cagagaggta cttttgaaca ccagcaccac ttcgttggaa taagaagaga gcgagcgttt
    19981 aaaggcatac ggcagcagga gcgccagcgt atcttttttg ctcattccca gggcgctaca
    20041 ggactgccac tgaccttctg ggatcgcacg aattgcaccg taaaacagct gcgtggtata
    20101 cgccgcactg ttcagcgata acgcaatcag cgcacataac cacggttctg acaacaaatg
    20161 ccacagtgcc ggatactcct gcaacgtcgg aaactggccc ggcccgtaat aaatcaggaa
    20221 gatctgcacc agcagcggcg taccggtaaa cagcgtgata taaccccgca ccagccacac
    20281 cagcaccggc gttttcagcg tcaggatgat ggtaaaaatc aatgccagaa tcagtgccac
    20341 aatcagcgag gcaacggtta gcgtcaggct ggtgtgtagc cctttcatca gttcgggtaa
    20401 atactcaaac attagctggg cctccgctca aaacgtgtcg cgcgcaggtc aatgcgtttg
    20461 agaatgtact gactgagcag ggtgatcacc aggtaaatcg ccgccgccac aatgtaccag
    20521 gtaaatggtt cctgggtacg agtagcgatg ctttttgttt gcagcattaa atcattcaca
    20581 ctaatcaaac tgaccagcgc ggtatctttc agcagcacca gccactggtt accgaggcca
    20641 ggcagcgcat gacgccacat ctgcggcatc accagacgga aaaagatagc cgatttcgac
    20701 agccccagcg cctgaccaga ctcccactga cccaccggca ccgctttcaa cgcgccccga
    20761 agcgtttgcg aggcataggc ggcatacagc agtgacagag cgatgacacc acaaaggaac
    20821 gggctaacgt cgaagttctc aatgtccatc tgcactggga tctgcacgaa cccaagattg
    20881 atagtgaagc catccgaaag cgtcagcagc agctgcgagg agccaaaata gataaacagc
    20941 accaccagaa tttctggcag gccacgcaga atggttacca gcgctgaacc tgcccacgcg
    21001 acaggacgcc attttgccga ctcccatacc gcaaagaaca tcgccagcgc cagcccgaca
    21061 atcaatgcac aaacggcaag gccgacggtc atcccggcgg cgcttgctaa aggaaaaaat
    21121 tcattcatca ggaattactt ctggaaccat ttgttgtaga tggtttcgta agtgccatct
    21181 ttcttcactt tttccagcgc agtgttgagt ttctgctgca gctcagtgtt gccctgacgt
    21241 accgcgatgc cgaggccagt gccgaagtaa tctttatcgg tcactttgtc gcccaccgcc
    21301 gccagtttcg ggttatcttt cagccactca gtgaccactg cggtgtcacc gaagacgccg
    21361 tcgatacgcc cgttttgcag atccagtttt gcgttctggt agctgtcata cggaacggta
    21421 gtgatttccg ggtgcttatc cataatgaat ttctggtgtg tcgtcccgtt ctgtacgccg
    21481 acttttttgc ctttcagctg atcaacactg gtgtatttgc cttgctgacc cacaaacagg
    21541 gcagagttgt catagtacgg ggtggtaaac agcacctgct tttcacgctc cggagtgata
    21601 tccatgcccg ccatcacggc ttctacgcga cggaatttca ggcttgggat caggctgtca
    21661 aacgcctggt tagagaaagt gcaggttgca tcaatctctt tacacagcgc ttgtgccagg
    21721 tcgacgtcaa aaccaacgat ctggttgttt gcatcaatcg attcaaacgg aggataggat
    21781 gcttcggtag caaaacgaat ggtttcggca gctgtggcgg aaagactaaa acctgcaatt
    21841 aacgcggcaa tcagaacttt tttcattgtt gttatcccga atcttagtga gagagatagt
    21901 ttttaaatgc ttcggtttgc ggctcggtaa agcagctcgc gtcgccttgt tctacgatat
    21961 gaccattttc catatacacc actcggctgg cggttttacg cgccacttca acttcgtggg
    22021 tgacgatcac ctgggtaata ttcgtttctg ccagctcacg aatgatgctg acgatttgtg
    22081 ccgtaatttc cgggtccagt gcggcggtcg gttcatcgaa cagcagtacc tgcggttcca
    22141 tcatcaacgc acgggcaata gcaacacgct gctgctgacc accagaaaga tgcagcgggt
    22201 aacgatcgct ataaggtttg agacgcagac gttccagcag tttttctgca cgggccagcg
    22261 cctgatcttt actcaacccc agtacacggc agggcgcttc aatcaggttt tgctgcacgg
    22321 tcagatgcgg ccacaggttg tattgctgaa acaccatgcc aacgttacga cgcaaatcgc
    22381 gaatcgcttt gtcagagggt gttttggtga aatcgaaatg gttgcctgca atgttgagcg
    22441 taccggagcg tggcatctca agcagattga gtacacgcag cagcgagctt ttacccgcgc
    22501 cgctggggcc aagtaacacc agcgtttcgc cctgtgggca atccagcgtg atatcgaaca
    22561 gcgcctgatg cgcgccgtag aagcaattaa tgccgtttaa ttgaatactc attgacactc
    22621 gtatactggc agtctgatag ctattgaggt cgaagatagt acctttgaca gaataattat
    22681 gcaatatttc tgctttaaaa gttaaaagca aagcgcatta ttcaataaac atagcacaaa
    22741 ataacggggg cggtggtcgg cgagcataaa tgtcggcatt cctcacgaaa tgccggacaa
    22801 tttacggggt ttattggttg atcaaggcgt tagcgattct cgatggactg acggagcgta
    22861 cccgccgtgg catgaacgct gccgcctaag tagcgcacat cgtcgatgac ccaacactgg
    22921 ccttcctgaa tcattaacac ttcatcctgc caaccctggt caccctgttt gagatctacg
    22981 cgcaatggaa tgttacgggc atcacgatta gggatagtcg atgcactggc aacgtgggcg
    23041 ctatctggca aggtggttcg actggagaac ggatcgctgc ttaacaattc acgatgacta
    23101 ttatcgcggc tggcatcgct aagaagggtt gccagtttgt cactcaaata ggggcgcagg
    23161 gcggtaatgt cattgctgcg atgttgaatg cggtagtcat agaattgttg agccacgctg
    23221 tcaggccccc cctggacaca ggaaccggtg cggggaccgt tatctttata agctggcgtg
    23281 acggtggtac aggcactcag cagcagtgca catggaataa gtagagatag tgaacggtag
    23341 cgcataatca tttccttatt agcttgctgt ctgcgtcaca ctacgaccaa acgctatcag
    23401 attatttaat acattgtttt taatacaatt ctgaaacaat ctgaacacct gattgggttt
    23461 gagtccattg ctggctatcc actcaaggtt gctcgtagct gacttactta aattacccaa
    23521 acaatagaca gtgtaaacca tttgtgaggt tgagcgtgtt atatggctga aatgatcatt
    23581 taaggtaaat atgatagttc tgaaatggtg gtataaatcc tcctgtagag gttaagagca
    23641 tctgcaagaa gccagtttca catttataac gcgtgataaa tgaacgcggt tattgtctaa
    23701 tcattacaaa atttttcaca tgcgataaga gatcctgcga ttaagatgat tgccaaaatt
    23761 acgttgtatt tttgaaaatg agcgaaattt gatagtccca gctttgactc tcgataatat
    23821 tatttatgct acaattgtgg ctaaagaatt taagctaatg aattttagcg aagaataaat
    23881 taagttgttt cagtatctcg ttaattaata tgaatataaa aggcgttgtg cttttataat
    23941 tataaagcac gaggagttcg gtggattatc gtttttcttg ggtgattttt aatatgataa
    24001 acaatatatc tgatcaagca tcatcatttc caggcacaca actaaatcaa agtgataatt
    24061 tcctggattc actgagggaa tttttcgcga tattaaattc cagtcgtaag ggtgagttgt
    24121 ctacatggga taccatctac ttgcatctga tattagcaat aaatgctgac agtgatctca
    24181 taaaaaatga tgtgttgctc gcggaaaata tgccatcagc gaattaccaa tttaatactt
    24241 tttttagtaa tgctttcgag actgatgtta aaaaatatct gggtaaatca gaagataacg
    24301 aagttgaaat aaaggcgggt aatgaaagaa tctctattgg aatacgtaat gtatcgaatg
    24361 ggaaacttga aagacaacag tttcttttcc ctctggacta tgagaataaa ttacaagatc
    24421 aattagacaa gcattttacg attgaatcgc acccattatt atacaggcat accattggta
    24481 gtaaaattgc gaatgtgatt tttgaaaaat tatacagccg tattgacttt aacaaagaac
    24541 aatatatatc ctttataaag ggcgcgttta ttcattttta tgattatagc aggcgatatg
    24601 caattagtga gaatatcgaa aaagacgctg taacaaacaa tatcgcattg atgaacactt
    24661 tttatgacag tgataatacg tcaggtgagg ttttaaataa tgattttaca gaagaagagc
    24721 catttgaaac tgcgttagat gtagagcata caatagtgct tggatttgct gatgataatt
    24781 ttgaaacaaa acctgttcat tatcaggatt tattaacacg atttagtgcc tttcaagata
    24841 ccgtgtttaa tctgtttcct gaaatgcgct cttcgcatta ccacgatatt tgtagcgtat
    24901 gtgtggatat gacaaaggga acgcaatgca tgattcactt gatggtgaat gaagaagttt
    24961 tcatgtctct gcctgttcca gttgccacga tggtacgtga agatgcaagt aatttagtca
    25021 atcttaaaac actactcaat gatggttttt tcattaaata tagtcatttt aatgacgtgg
    25081 cattaataaa gcaaaacata agtaatctct acttatcaca cactgttgtg aatgaaagca
    25141 tattgaaaaa atgttgtttt gaaaacggca gtttaggt
//
LOCUS       DAEEKQ010000049        25104 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.049, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000049 DAEEKQ010000000
VERSION     DAEEKQ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.049"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            194..382
                     /locus_tag="KLK57_001895"
     CDS             194..382
                     /locus_tag="KLK57_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HBE0892271.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            394..1146
                     /gene="bcsQ"
                     /locus_tag="KLK57_001896"
     CDS             394..1146
                     /gene="bcsQ"
                     /locus_tag="KLK57_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HBE0892272.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            1143..1307
                     /locus_tag="KLK57_001897"
     CDS             1143..1307
                     /locus_tag="KLK57_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HBE0892273.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPR"
     gene            1500..3761
                     /gene="bcsA"
                     /locus_tag="KLK57_001898"
     CDS             1500..3761
                     /gene="bcsA"
                     /locus_tag="KLK57_001898"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HBE0892274.1"
                     /translation="MNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSLILA
                     LICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRYTST
                     LNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVDIFV
                     PTYNENLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIARTTHE
                     HAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHFFSP
                     DPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSYAVIRRKPLDEIGGIAVET
                     VTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLDNPL
                     TGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFVLPH
                     MIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGGLVE
                     EEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVLGGA
                     VAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQILEGQ
                     KVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWALWQD
                     SYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRHPERS
                     ETAQPSDQALAQQ"
     gene            3772..6111
                     /gene="bcsB"
                     /locus_tag="KLK57_001899"
     CDS             3772..6111
                     /gene="bcsB"
                     /locus_tag="KLK57_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HBE0892275.1"
                     /translation="MKRKLFWICAVAMRMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKSDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKTPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            6118..7224
                     /gene="bcsZ"
                     /locus_tag="KLK57_001900"
     CDS             6118..7224
                     /gene="bcsZ"
                     /locus_tag="KLK57_001900"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HBE0892276.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVIA
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPIGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            7206..10698
                     /gene="bcsC"
                     /locus_tag="KLK57_001901"
                     /pseudo
     CDS             7206..10698
                     /gene="bcsC"
                     /locus_tag="KLK57_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
     gene            10780..12768
                     /gene="hmsP"
                     /locus_tag="KLK57_001902"
     CDS             10780..12768
                     /gene="hmsP"
                     /locus_tag="KLK57_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HBE0892277.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPIMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFAHPLPIEIFEESYLEEK"
     gene            12951..14237
                     /gene="dctA"
                     /locus_tag="KLK57_001903"
     CDS             12951..14237
                     /gene="dctA"
                     /locus_tag="KLK57_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HBE0892278.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            14457..15953
                     /locus_tag="KLK57_001904"
     CDS             14457..15953
                     /locus_tag="KLK57_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBE0892279.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNIGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="KLK57_001905"
     CDS             complement(16049..16978)
                     /gene="kdgK"
                     /locus_tag="KLK57_001905"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HBE0892280.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVCLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            17210..17977
                     /gene="pdeH"
                     /locus_tag="KLK57_001906"
     CDS             17210..17977
                     /gene="pdeH"
                     /locus_tag="KLK57_001906"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HBE0892281.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHDLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            18047..20107
                     /locus_tag="KLK57_001907"
     CDS             18047..20107
                     /locus_tag="KLK57_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBE0892282.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWFKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIATNIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPTAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(20289..21611)
                     /locus_tag="KLK57_001908"
     CDS             complement(20289..21611)
                     /locus_tag="KLK57_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBE0892283.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="KLK57_001909"
     CDS             complement(22022..23035)
                     /gene="yhjD"
                     /locus_tag="KLK57_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HBE0892284.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(23084..23983)
                     /locus_tag="KLK57_001910"
     CDS             complement(23084..23983)
                     /locus_tag="KLK57_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0892285.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAMVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGQLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEYLD"
     gene            24503..>25104
                     /locus_tag="KLK57_001911"
     CDS             24503..>25104
                     /locus_tag="KLK57_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE0892286.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKQES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRTTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
BASE COUNT         5895 a         6365 c         6991 g         5853 t
ORIGIN      
        1 ttagcttgtg aatcaattta gaaggtcaca agacgtgtga ttttagtctt ttcgttttta
       61 actttttctg ttgcgtaacg gaaagtcaaa aagtgagcaa attcccgtct cgccgtaaac
      121 aataaaccgg cgaaatgctc acggttagaa ttgtttcatg aacggtacgg ttatttcata
      181 gggatcaagc aaaatgaata acaatgaacc agatactctg cctgatcccg cgataggcta
      241 tatcttccag aatgatattg tggcgttaaa gcaggcattt tcactgcctg atattgatta
      301 tgccgatatt tcccaacgcg aacagttggc cgcggcatta aaacgctggc cgttgctggc
      361 agagtttgcg caacaaaagt aggggattgg tgaatggccg tactgggatt gcagggggtg
      421 cggggaggcg tggggacaac aaccatcacc gccgcattag cctggtcatt acaaatgttg
      481 ggagaaaatg tcctggtggt cgatgcctgc ccggacaact tgttgcgcct gtcatttaat
      541 gttgatttta cccaccgtca gggctgggcc agagcgatgc tggatggcca ggactggcgt
      601 gacgctgggt tgcgctacac ctcgcagctc gatttgctgc cttttggtca gttatccatt
      661 gaagaacaag aaaatccaca gcactggcaa acccggctga gcgatatttg ctccggctta
      721 cagcaactaa aagccagcgg gcgttaccag tggattttaa tcgacttacc gcgtgatgcc
      781 tcgcagataa cccaccagct gctgagtttg tgcgatcact cgctggcaat cgtcaatgtg
      841 gatgccaact gccatatccg actgcatcag caagcgctgc cggatggcgc acatattttg
      901 attaatgact tccgtattgg cagtcaggtt caggacgata tttaccagct ttggttgcaa
      961 agccagcgcc gattactgcc gatgctcatt catcgtgatg aagcgatggc tgaatgcctg
     1021 gcggctaagc aaccagtagg tgaatatcgc agtgatgcgc tggcggctga agagatactg
     1081 acgctggcga actggtgcct gttgaactac tccgggctga aaacgccagt cgggagtgca
     1141 tcatgagtat cctgacccgg tggttgctta tcccgccggt caacgcgcgg cttatcgggc
     1201 gttatcgcga ttatcgtcgt cacggtgcgt cggctttcag cgcgacgctc ggctgtttct
     1261 ggatgatcct ggcctggatt tttattccgc tggagcaccc gcgctagcag cgtattcgcg
     1321 cagaacataa aaacctgtat ccgcatatca acgcctcgcg tccgcgtccg ctggacccgg
     1381 tccgttatct cattcaaaca tgctggttac tgatcggtac atcgcgcaaa gaaacgccga
     1441 aaccgcgcag gcgggcattt tcaggtctgc agaatattcg tggacgttac catcaataga
     1501 tgaacgagct gcctgagcgc gttagccata aaacacagca tcttgatgag aaaaaagagc
     1561 tcggtcattt gagtgccggg gcgcggcggt tgatcctcgg tatcatcgtc accttctcgc
     1621 tgattctggc gttaatctgc gttactcaac cgtttaaccc gctggcgcag tttatcttcc
     1681 tgatgctgct gtggggtgta gcgctgatcg tacggcggat gccggggcgc ttctcggcgc
     1741 taatgttgat tgtgctgtcg ctgaccgttt cttgccgtta tatctggtgg cgttacacct
     1801 ctacgctgaa ctgggacgat ccggtcagcc tggtgtgcgg gcttattctg ctcttcgctg
     1861 aaacgtacgc gtggattgtg ctggtgctcg gctacttcca ggtagtatgg ccgctgaatc
     1921 gtcagccggt gccattgccg aaggatatgt cgctgtggcc gtcagtggat atctttgtcc
     1981 cgacttacaa cgaaaatctc aatgtggtga aaaataccat ttacgcctcg ctgggtatcg
     2041 actggccgaa agacaaactg aacatctgga tcctcgatga cggcggcagg gaagagtttc
     2101 gccagtttgc gcaaaacgtg ggggtgaaat atatcgcccg caccactcat gaacatgcga
     2161 aagcgggcaa catcaacaat gcgctgaaat atgccaaagg cgagttcgtg tcgattttcg
     2221 actgcgacca cgtaccaacg cgatcgttcc tgcaaatgac catgggctgg ttcctgaaag
     2281 agaaacagct ggcgatgatg cagactccac accacttctt ctcgccggac ccgtttgaac
     2341 gcaacctggg acgttttcgt aaaacgccga acgaaggcac gctgttctat ggtctggtgc
     2401 aggatggcaa cgatatgtgg gacgccactt tcttctgcgg ttcctatgcg gtgattcgtc
     2461 gtaagccgct ggatgaaatt ggcggcattg ctgtcgaaac cgtgactgaa gatgcgcata
     2521 cttctctgcg tttgcaccgt cgtggctata cctccgcgta tatgcgtatt ccgcaggcgg
     2581 cggggctggc gaccgaaagt ctgtcggcgc atatcggtca gcgtattcgc tgggcgcgcg
     2641 ggatggtaca aatcttccgt ctcgataacc cgctcaccgg taaaggactg aagtttgctc
     2701 agcggctgtg ctacgtcaac gccatgttcc acttcttgtc gggcattcca cggctgatct
     2761 tcctgactgc gccgctggcg ttcctgctgc ttcatgccta catcatctat gcgccagcgt
     2821 tgatgatcgc cctattcgtg ctgccgcata tgatccatgc cagcctgacc aactcgaaga
     2881 tccagggcaa atatcgccac tctttttgga gtgaaatcta cgaaacggtg ctggcgtggt
     2941 atatcgcacc accgacgctg gtggcgctga ttaacccgca caaaggcaaa tttaacgtca
     3001 ccgccaaagg tggactggtg gaagaagagt acgtcgactg ggtgatctcg cggccctaca
     3061 tcttccttgt tctactcaac ctggtgggcg ttgcggtagg catctggcgc tacttctatg
     3121 gcccgccaac cgagatgctc accgtggtcg tcagtatggt gtgggtgttc tacaacctga
     3181 ttgttcttgg cggcgcagtt gcggtatcgg tagaaagcaa acaggtacgc cgatcgcacc
     3241 gcgtggagat gacgatgccc gcggcaattg cccgcgaaga tggtcacctc ttctcgtgta
     3301 ccgttcagga tttctccgac ggtggtttgg ggatcaagat caacggtcag gcgcagattc
     3361 tggaagggca gaaagtgaat ctgttgctta aacgcggtca gcaggaatac gtcttcccga
     3421 cccaggtggc gcgcgtgatg ggtaatgaag ttgggctgaa attaatgccg ctcaccaccc
     3481 agcaacatat cgattttgtg cagtgtacgt ttgcccgtgc ggatacatgg gcgctctggc
     3541 aggacagcta cccggaagat aagccgctgg aaagtctgct ggatattctg aagctcggct
     3601 tccgtggcta ccgccatctg gcggagtttg cgccttcttc ggtgaagggc atattccgtg
     3661 tgctgacttc tctggtttcc tgggttgtat cgtttattcc gcgccacccg gagcggagcg
     3721 aaacggcaca accatcggat caggctttgg ctcaacaatg atgataacgc gatgaaaaga
     3781 aaactattct ggatttgtgc agtggctatg aggatgagtg cgttcccctc tttcatgacg
     3841 caggcgacgc cagcaacgca accactgatc aatgctgagc cagctgtagc cgcccagacg
     3901 gaacaaaatc cgcaggtggg gcaagtgatg ccgggcgtgc agggcgctga tgcgccagtc
     3961 gtggcgcaga acggtccttc gcgtgatgtg aagctgacct ttgcgcaaat tgcgccgccg
     4021 ccgggcagca tggtgctacg tggcattaac ccgaacggca gcattgagtt tggtatgcgc
     4081 agcgatgaag tggtgacgaa ggcgatgctc aacctcgaat acacgccatc gccatcgtta
     4141 ctgcctgtcc agtcgcagtt aaaggtttat ctcaatgatg agctgatggg cgtgctgcca
     4201 gtgaccaaag aacagttggg taaaaaaacg ctggcgcaaa tgcccattaa cccactgttt
     4261 attaccgact tcaaccgtgt gcggctggag tttgtcggcc attatcagga cgtgtgcgaa
     4321 aacccggcca gcaccacgct ttggctggat gttgggcgga gcagtggact ggatctgacc
     4381 tatcagaccc tgaatgtgaa gagtgacctg tcacacttcc cggtgccatt ctttgacccg
     4441 cgcgataacc gtactaacac cttgccgatg gtctttgcgg gtgcgccgga tgttgggctg
     4501 caacaagcat ctgccattgt cgcctcgtgg tttggttcgc gttctggctg gcgtgggcag
     4561 aacttcccgg tgctctataa ccaattgccg gatcgcaatg ccattgtctt tgcaaccaac
     4621 gacaaacggc cggacttcct gcgcgatcat ccggcggtaa aagccccggt gattgagatg
     4681 attaaccatc cgcagaatcc ttacgtcaaa ctgctggtgg tgtttggtcg tgacgacaaa
     4741 gacctgttgc aggcagcgaa aggtatcgct cagggtaaca ttctgttccg tggtgaaagc
     4801 gtggtagtga atgaagtgaa accgctgcta ccgcgtaagc cgtacgatgc gccgaactgg
     4861 gtacgtaccg atcgtccggt cacatttggc gaactgaaaa cctatgaaga acagttacaa
     4921 tccagcggtc ttgagccagc agcgattaac gtttcgctaa acctgccgcc ggatctctac
     4981 ctgatgcgca gtaccggcat tgatatggat attaattacc gctacaccat gccgccggtg
     5041 aaagacagtt cgcggatgga tatcagcctg aataaccagt tcctgcaatc cttcaacctg
     5101 agcagtaaac aggaggcgaa ccgcctgctg ctgcggattc cggtattaca aggtttgctg
     5161 gatggcaaaa cggatgtctc tattccggcg ctgaaactgg gcgcgaccaa ccagctgcgc
     5221 ttcgactttg agtatatgaa cccgatgccg ggcggttcgg tggataactg tattaccttc
     5281 cagccggtgc agaatcatgt ggtgattggt gacgattcca ccatcgactt ctcgaagtat
     5341 taccacttca tcccgatgcc ggatctacgc gcctttgcta acgcgggctt cccgttcagc
     5401 cggatggcgg atctgtcgca aaccatcacc gtgatgccga aaacgcctaa cgaagcacag
     5461 atggaaacgt tgctgaatac tgttggtttt atcggcgcac agacgggctt cccggcgatt
     5521 aatctgacgg tgaccgatga tggcagcacc attcagggca aagatgccga catcatgatc
     5581 atcggtggta tcccggacaa attaaaagac gataagcaga tcgacctgtt ggtgcaggcg
     5641 accgaaagct gggtgaaaac accgatgcgc cagaccccgt tccccggcat tgtgccggac
     5701 gagagcgatc gtgcggcaga aacccagtca acgctgacct cttccggtgc gatggcggcg
     5761 gtgattggct tccagtcgcc gtataacgac cagcgcagcg tgattgcgct gctggcagat
     5821 agcccacgcg gttatgaaat gcttaacgat gcggtgaacg atagcggcaa acgcgccacc
     5881 atgttcggtt cggtcgcggt gatccgcgag tccggtatca acagcctacg tgttggcgac
     5941 gtttattacg taggtcatct gccgtggttc gagcgcttgt ggtatgcgct ggcaaaccat
     6001 ccgattctgc tggcggtgct ggcggcaatc agtgtgatat tgctggcatg ggtactgtgg
     6061 cgtctgctgc gaattattag tcgtcgtcgt cttaacccgg ataacgagta attgaagatg
     6121 aatgtgttgc gtagtggaat cgtgacgatg ctgctgttgg ctgcctttag tgttcaggca
     6181 gcctgtacct ggcctgcctg ggagcagttt aaaaaggatt acatcagtca ggaagggcgc
     6241 gtcatcgccc ccagcgacgc gcgcaaaatc accacctccg aagggcaaag ttacggtatg
     6301 ttctttgccc tggcggctaa cgaccgtgta gctttcgata atattctcga ctggacgcag
     6361 aacaatctcg ctcagggctc tttaaaagaa cgtttgcccg cctggctgtg gggcaagaaa
     6421 gagaacagta agtgggaagt gctggacagc aattcggcct ccgatggtga tgtctggatg
     6481 gcctggtcgt tgctggaggc ggggcgtttg tggaaagagc agcgttatac cgacatcggc
     6541 agcgcgttgc taaaacgtat cgcgcgggag gaagtggtga cggtgcctgg gctgggttcc
     6601 atgttgttac cgggcaaagt gggttttgct gaggataaca gctggcgttt taaccccagc
     6661 tacctgccgc cgacgctggc gcagtatttc acccgctttg gcgcgccgtg gaccacgctg
     6721 cgcgaaacca atcaacgttt attgctggaa accgccccga aaggtttttc gccagactgg
     6781 gtgcgctatg agaaagacaa aggctggcag ctaaaagccg aaaaaacatt gatcagcagc
     6841 tacgacgcta tccgcgttta catgtgggta ggcatgatgc ctgacagcga tccgcaaaaa
     6901 gcgcggatgc tcaaccggtt taaaccgatg gcgacattca ctgagaaaaa cggttatccg
     6961 ccggaaaaag tggatgtggc tacggggaaa gcgcagggta aaggaccgat cggtttttct
     7021 gccgccatgc tgcccttttt acaaaaccgc gatgcgcagg ccgttcagcg ccagcgcgtg
     7081 gccgataact ttcccggcag cgatgcctat tacaactatg tgctgaccct gtttggacaa
     7141 ggctgggatc aacaccgttt ccgcttctcg acaaaaggtg agttattacc tgactggggc
     7201 caggaatgcg caaattcaca ctaaacatat tcacgctttc cctcggtctg gccgtcatgc
     7261 cgatggtcga ggcagcacca accgctcagc aacagttgct ggagcaagtt cggttaggcg
     7321 aagcgaccca tcgtgaagat ctggtgcaac agtcgttata tcggctggaa cttattgatc
     7381 cgaataaccc ggacgtcgtt gccgcccgtt tccgttcttt gttacgtcag ggcgatattg
     7441 atggcgcgca aaaacagctc gatcggctgt cgcagttagc gccgagttca aatgcgtata
     7501 aatcgtcgcg gactacgatg ctactttcca cgccggatgg tcgtcaggca ctgcaacagg
     7561 cacgattgca ggcgacgacc ggtcatgcag aagaagctgt ggcgagttac aacaaactat
     7621 tcaacggtgc gccgccggaa ggtgacattg ctgtcgagta ctggagtacg gtggcgaaaa
     7681 ttccggctcg ccgtggcgaa gcgattaatc agttaaaacg catcaatgcg gatgcaccgg
     7741 gcaatacggg cctgcaaaac aatctggcgc tattgctgtt tagtagcgat cgccgtgacg
     7801 aaggttttgc cgtcctggaa cagatggcaa aatcgaacgc cgggcgcgaa ggggcctcta
     7861 aaatctggta cgggcagatt aaagacatgc ccgtcagtga tgccagtgtg tcggcgctga
     7921 aaaaatatct ctcgatcttt agtgatggcg atagcgtggc ggctgcgcaa tcgcaactgg
     7981 cagaacagca aaaacagctg gccgatcctg ctttccgcgc tcgtgcgcaa ggtttagcgg
     8041 cggtggactc tggtatggcg ggtaaagcca ttcccgaact acaacaggcg gtgcgggcga
     8101 acccgaaaga cagtgaagct ctgggggcgc tgggccaggc gtattctcag aaaggcgatc
     8161 gcgccaatgc agtggcgaat ctggaaaaag ccctcgcact ggacccgcac agcagcaaca
     8221 acgacaaatg gaacagtctg ctgaaagtaa accgctactg gctggcgatc cagcagggcg
     8281 atgctgcgct gaaagccaat aatcctgacc gggcagaacg cctgttccag caggcgcgta
     8341 atgtcgataa caccgacagt tatgcagtgc tggggctggg cgatgtggcg atggcgcgaa
     8401 aagattatcc cgccgccgaa cgttattatc agcagacctt gcgtatggac agcggcaaca
     8461 ctaacgccgt gcgcgggctg gcaaatattt accgccagca atcgccagaa aaagctgaag
     8521 cgtttatcgc ctcgctctct gccagtcagc ggcgtagcat tgatgatatc gaacgcagcc
     8581 tgcaaaacga ccgtctggca cagcaggcag aggcactgga aaaccagggc aaatgggcgc
     8641 aggcggcagc acttcagcgg caacgactgg cgctggaccc cggcagcgta tggattactt
     8701 accgactttc gcaggatctc tggcaggccg gacaacgcag ccaggccgat acgttaatgc
     8761 gcaatctggc gcagcagaag tcgaacgacc cagagcaggt ttacgcttac gggctgtacc
     8821 tacgcttacg ggctgtacct ctctggtcat gaccaggaca gagcggcgct ggcgcatatc
     8881 aacagcctgc cgcgtgcgca gtggaacagc aatattcagg agctggttaa tcgactgcaa
     8941 agcgatcagg tgctggaaac cgctaaccgc ctgcgagaaa gcggcaaaga ggcagaagcg
     9001 gaagcgatgc tgcgccagca accaccttcc acgcgtattg acctcacgct ggctgactgg
     9061 gcgcaacaac gacgtgatta caccgccgcc cgcgctgcat atcagaatgt cctgacgcgg
     9121 gagccagcta acgccgacgc cattcttggt ctgacggaag tggatattgc tgccggtgac
     9181 aaagcggcgg cacgtagcca gctggcgaaa ctgcccgcta ccgataacgc ctcgctgaac
     9241 acacagcggc gcgtggcgct ggcacaggcg cagcttggcg ataccgcagc agcgcagcgg
     9301 acgtttaata agttgatccc gcaggcaaaa tctcagccac cgtcgatgga aagcgcgatg
     9361 gtgctgcgtg atggtgcgaa gtttgaagcg caggcgggcg atccaacgca ggcgctggaa
     9421 acctacaaag acgccatggt cgcatccggt gtgactacga cgcgtccgca ggataacgac
     9481 acctttaccc gactgacccg taacgacgag aaagatgact ggctgaaacg tggcgtgcgc
     9541 agcgatgcgg cggacctcta tcgccagcag gatcttaacg tcacccttga gcacgattac
     9601 tggggttcga gcggcaccgg tggttactcc gatctgaaag cgcacactac catgttgcag
     9661 gtggatgcgc cgtattctga cgggcggatg ttctttcgca gtgatttcgt caatatgaac
     9721 gtcggcagtt tctccactaa tgccgatggc aaatgggatg acaactgggg cacctgtaca
     9781 ttacaggact gtagcggcaa ccgcagccag tcggattccg gtgccagcgt ggcggtcggc
     9841 tggcgaaatg acgtctggag ctgggatatc ggtaccacgc cgatgggctt caacgtggtg
     9901 gatgtggtcg gcggcatcag ttacagcgat gatatcgggc cgctgggtta caccgttaac
     9961 gcccaccgtc ggcccatctc cagttctttg ctggcctttg gtgggcaaaa agactccccg
    10021 agcaataccg ggaaaaaatg gggtggcgta cgtgccgacg gtgtggggct aagtctgagc
    10081 tacgataaag gtgaagcaaa cggcgtctgg gcatcgctta gtggcgacca gttaaccggt
    10141 aaaaatgtcg aagataactg gcgcgtgcgc tggatgacgg gctattacta taaggtcatt
    10201 aaccagaaca atcgccgcgt cacaatcggc ctgaacaaca tgatctggca ttacgacaaa
    10261 gatctgagtg gctactcact cggtcagggc ggttactaca gtccgcagga atacctgtcg
    10321 tttgccatac cggtgatgtg gcgggagcgc acggaaaact ggtcgtggga gctgggggcg
    10381 tctggctcgt ggtcgcattc acgcaccaaa accatgccgc gttatccgct gatgaatctg
    10441 atcccgaccg actggcagga agaagctgcg cggcaatcca acgatggcgg cagcagtcag
    10501 ggcttcggct acacggcgcg ggcattactt gaacgacgtg ttacttccaa ctggtttgtt
    10561 ggcacggcaa ttgatatcca gcaggcgaaa gattacgcac ccagccattt cctgctctac
    10621 gtacgttatt ccgccgccgg atggcagggt gacatggatt taccgccgca gccgctgata
    10681 ccttacgccg actggtaagt tttcagatag cgcctctctt aatgccgctg cgatcgggta
    10741 tactcgggcg gcaatctggg atttccgggg ggagacaatt tgcgcgtaag tcgctcgtta
    10801 acaatcaagc agatggcaat ggtggcagcc gttgtcctgg tgttcgtttt tattttttgc
    10861 accgttttgc tgttccatct ggtccagcag aatcgctata acacggctac gcaactggaa
    10921 agcattgctc gctctgtccg cgaaccctta tcttcagcta ttttgaaagg cgatattccc
    10981 gaagcggaag ctattcttgc cagcattaaa ccggcaggcg tggtcagccg tgccgatgta
    11041 gtgctgccta accagttcca ggcgctgcgt aaaagtttta ttccagagcg cccggtgccg
    11101 ataatggtta ctcgcctgtt tgagctaccg gttcaaatct cgctgggcgt ttactcgctc
    11161 gaacgtccgg caaacccgca gccaattgcc tatctggtac tacaggcgga ttccttccgt
    11221 atgtataagt tcgtgatgag caccctctca acgttagtga ccatttactt acttttgtcg
    11281 cttatcctga ccgtcgccat cagctggtgc attaaccgcc tgattttgca tccgttacgc
    11341 aatattgctc gcgaacttaa cgccatccca gcccaggagc ttgttggtca ccaactggca
    11401 ttaccgcgtc tgcatcagga cgatgaaatc ggtatgttgg tgcgcagtta caacctcaac
    11461 cagcaattgc tgcagcgcca ttatgaagaa cagaacgaaa atgcgatgcg cttcccggtg
    11521 tcggatttgc cgaacaaagc cttgctgatg gagatgctgg agcaggttgt cgcgcgtaaa
    11581 caaaccaccg cgctgatgat catcacctgt gaaaccctgc gtgatactgc gggcgtgctg
    11641 aaagaggcgc aacgagaaat tctgctgctg acgctggtgg aaaaactcaa atcggtactg
    11701 tcgccacgta tgatcctcgc gcagattagc ggttatgact ttgctgtcat tgccaacggt
    11761 gtacaggaac cgtggcacgc aatcacctta ggtcagcaag tgctcaccat catgagcgag
    11821 cgcctgccga ttgaacgtat tcaactccgt ccgcactgta gcattggcgt ggcgatgttc
    11881 tacggcgatc tcaccgccga acagctttac agtcgcgcta tttctgcggc atttaccgct
    11941 cgccataaag gcaagaatca gattcagttc tttgatccgc agcagatgga agccgcccag
    12001 aagcggttga cggaagagag cgatatcctt aatgcactgg aaaatcatca gtttgctatt
    12061 tggttacagc cacaggtcga gatgaccagc ggtaaactgg tcagtgcgga agtgttactg
    12121 cgtatccagc aaccggatgg cagttgggac ctgccggatg gcttaatcga tcgcattgag
    12181 tgctgtgggc tgatggttac cgtcggtcac tgggtgctgg aagagtcctg tcgattgctt
    12241 gcagcctggc aagagcgcgg cattatgctg cccttgtcgg taaacctctc tgcgctgcaa
    12301 ctgatgcacc caaatatggt ggcggatatg ctggaactgt taacccgcta tcgcattcag
    12361 ccgggaacac tgattctgga agtgacagaa agccgacgta ttgacgaccc tcatgctgcg
    12421 gtggcaatcc tccgtccgct gcgcaatgcc ggagttcggg tggcgctgga tgatttcggc
    12481 atgggctacg cagggctgcg tcagctgcag catatgaaat cgttgccaat cgacgtactg
    12541 aaaatcgaca aaatgtttgt tgaaggcttg ccggaagata gcagcatgat tgctgcaatt
    12601 atcatgctgg cgcagagcct gaacttacaa atgattgccg aaggcgtgga gactgaagca
    12661 caacgcgact ggctggcaaa agcgggcgtt ggtattgccc agggcttcct ttttgctcac
    12721 ccactcccta ttgaaatctt cgaagagagt tacctggaag aaaagtagct accccaaact
    12781 gattacaaaa ctttaaaaag tgctggtttg tgcgagccag ctcaaacttt ttaacctttt
    12841 tgtttcaatt atgatccagg tacatttctg tgatgttgtc tgggtgttat tttaaggccg
    12901 caggtacccc ataaccttac aagacctgtg gttttactaa aggacaccct atgaaaacct
    12961 ctctgtttaa aagcctttac tttcaggtcc tgacagcgat agccattggt attctccttg
    13021 gccatttcta tcctgaaata ggcgagcaaa tgaaaccgct tggcgacggc ttcgttaagc
    13081 tcattaagat gatcatcgct cctgtcatct tttgtaccgt cgtaacgggc attgcgggca
    13141 tggaaagcat gaaggcggtc ggtcgtaccg gcgcagtcgc actgctttac tttgaaattg
    13201 tcagtaccat cgcgctgatt attggtctta tcatcgttaa cgtcgtgcag cctggtgccg
    13261 gaatgaacgt cgatccggca acgcttgatg cgaaagcggt agcggtttac gccgatcagg
    13321 cgaaagacca gggcattgtc gccttcatta tggatgtcat cccggcgagc gtcattggcg
    13381 catttgccag cggtaacatt ctgcaggtgc tgctgtttgc cgtactgttt ggttttgcgc
    13441 tccaccgtct gggcagcaaa ggccaactga tttttaacgt catcgaaagt ttctcgcagg
    13501 tcatcttcgg catcatcaat atgatcatgc gtctggcacc tattggtgcg ttcggggcaa
    13561 tggcgtttac catcggtaaa tacggcgtcg gcacactggt gcaactgggg cagctgatta
    13621 tctgtttcta tattacctgt atcctgtttg tggtgctggt attgggttca atcgctaaag
    13681 cgactggttt cagtatcttc aaatttatcc gctacatccg tgaagaactg ctgattgtac
    13741 tggggacttc atcttccgag tcggcgctgc cgcgtatgct cgacaagatg gagaaactcg
    13801 gctgccgcaa atcggtggtg gggctggtca tcccgacagg ctactcgttt aaccttgatg
    13861 gcacatcgat atacctgaca atggcggcgg tgtttatcgc ccaggccact aacagtcaga
    13921 tggatatcgt ccaccaaatc acgctgttaa tcgtgttgct gctttcttct aaaggggcgg
    13981 caggggtaac gggtagtggc tttatcgtgc tggcggcgac gctctctgcg gtgggccatt
    14041 tgccggtagc gggtctggcg ctgatcctcg gtatcgaccg ctttatgtca gaagctcgtg
    14101 cgctgactaa cctggtcggt aacggcgtag cgaccattgt cgttgctaag tgggtgaaag
    14161 aactggacca caaaaaactg gacgatgtgc tgaataatcg tgcgccggat ggcaaaacgc
    14221 acgaattatc ctcttaatct caccacttat gcccgcactc ccttccccga gtgcgggtgt
    14281 ttgcgcataa ttgacccctg tgcctgaact taccacctgg gtgagttcag gtttatttca
    14341 cttcttccgt gacattttca tgttcttgcg gtctaacacg aagtgttttt aacgtcattt
    14401 agactgactg acaacgggca gttttttatt accaggatag ttgatcaggg gttcacatgc
    14461 agggcacaaa aattcgactt ttagcgggcg gtttgctgat gatggccact gctggctatg
    14521 tgcaggcaga tgcgctccag cctgatccag catggcaaca ggggacgctt tccaacggtt
    14581 tacagtggca agtgctgacc accccccagc gtcccagcga tcgtgttgaa attcgcctgc
    14641 tggttaatat cggttcgctc gccgaaagta cacaacagag cggttacagt cacgccatcc
    14701 ctcgtattgc gctaacgcaa agcggtggcc ttgacgcagc acaggcgcgt tcattgtggc
    14761 agcaggggat cgaccctaaa cgcccgatgc cgccggtaat tgtctcttat gacaccacgc
    14821 tgtttaatct gagtttgccc aataaccgta acgatttgct gaaagaagcg ctctcttatc
    14881 tggcaaatgc cactggcaaa ttgaccatca caccagaaac catcaaccac gcgctgcaaa
    14941 gtcaggacat ggtggcaacc tggcctgccg atactaaaga gggctggtgg cgctatcgtc
    15001 tgaaagggtc aaccttgtta ggtcacgatc ctgccgatcc gctgaaacaa cccgttgaag
    15061 cggaaaaaat taaagatttc tatcagaaat ggtacacccc ggatgcaatg acgctactgg
    15121 tggtgggaaa cgtggatgcg cgctcggttg tcgaccaaat caacaaaacg tttggcgaac
    15181 tgaaaggcaa acgtgaaacg ccagctccgg tgccgacgct ttctccgctg cgtgcggaag
    15241 cggtgagtat tatgactgac gcggtgcgtc aggaccggtt atctatcatg tgggatacgc
    15301 cgtggcagcc gattcgtgaa tcagccgcac tgctgcgcta ctggcgtgcg gacctggccc
    15361 gcgaggcgct gttctggcat gttcagcaag cgttaagcgc cagtaacagc aaagacatcg
    15421 gtcttggatt tgactgccgt gtgctgtatc tgcgtgcgca gtgtgccatc aacatcgaat
    15481 caccaaacga caagctgaac agcaacctta atctggtggc gcgtgaactg gcgaaggttc
    15541 gcgataaagg tctgccggaa gaagagttca atgcgttagt ggcgcaaaag aaactggagc
    15601 tgcagaaact gtttgccgcc tatgcgcggg ctgataccga tattctgatg ggtcagcgga
    15661 tgcgttcgct gcaaaatcag gtcgtcgata tcgcgccgga gcagtatcag aaactgcggc
    15721 aggatttcct taacagcctg acggtggaga tgttaaatca ggatctgcgt cagcagttgt
    15781 cgaatgatat ggcgttaata ctgctgcagc cgaaaggcga gccggaattt aacatgaaag
    15841 cgttgcaggc ggcctgggat caaatcatgg ccccatcgac tgctgctgcc gccacctctg
    15901 tcgccacgga tgacgtacat cctgaagtga cggatattcc acctgcacag taatgcctga
    15961 tggtgatgag ttagcacaac catcaggtat ttatggatga gctggataga ggggtaacac
    16021 tttatccagc cttttgcata tgctgcgttt acgctggcat cgcctcacgc gggataatcg
    16081 cgccgcgata ctgaataacg gtacttgcgg tcaggtgccc gcgtttcgcc gcgttttccg
    16141 cactgccgcc cgtcaaacat accgccagat aaccggcgct gaaagagtcg ccagctgcgg
    16201 tggtatcgat cactttttct ttcggcagtt tcaccgccgg aacatccact aacccttcgc
    16261 cagcaatgga caccaggcaa gaatccgccc cgcgtttcac caccacttct ttcacgcccg
    16321 cgttatgggt gcgcgcaatg acgtcttcca ccggctgttg accccacagc gcgtcttcat
    16381 cgtccagcgt caggaaggcg atatccgtgc attccagcat ttgttggtac acctgctgtg
    16441 tctcttcttt gctggcccac aggcgcggac gatagttatt gtcgaaaatc acttttccgc
    16501 cgttggcgcg gcattcgcgc agcagggaaa gcagcttttc gcggctggtc gggcttaaga
    16561 tcgccaggct aatcccgctc aggtagagat aatcgaaatt cgccagctct tcgcaaatcg
    16621 ccgcagactg ctcactctcc agccagaatt tggcggcggc ttcgttccgc cagtagtaga
    16681 acgtacgctc gccggtgctg tcggtttcaa tgtagtaaag gcccggcaga cggttttcca
    16741 tccgttgggt cagggaagta tcaacgttct cgccgtgcca ggcgtccagc atttgctggc
    16801 taaaactgtc cgttcccagc gccgttacgt aatgaacggt taatgccgca ggatcgacct
    16861 gacgggcgat atagacggaa gtgttcaggg tatcgccgcc gaaaccgcgc ttaacgtccg
    16921 cgcctttctc ggaaagctca atcatgcatt cgccaatcac ggcaatcttt ttggacatag
    16981 tcgtgaacct gatctgtaaa aaattagcgt tagtttgcgc tgtggtggtt tgctggtcaa
    17041 ccatattaaa acagtgttcc attatttttt tgagccagaa cgtattcctg aaagattccg
    17101 ttgtggagtg aggaaattta ccccggccag gcggtaaagt tctgcccttg cgcgccgata
    17161 atctttgtcg agtccgggca gcatcacttt taaacacagg acatctttga tgataaggca
    17221 ggttatccag cgaataagca accctgaagc aagcatcgag agcttgcagg aacggcgttt
    17281 ttggttgcag tgtgagcgtg cttacacctg gcagccgatc tatcaaacat gcggacggtt
    17341 aatggccgtg gagctattaa cggtggtcac gcatcccttg aacccttcgc aacgcctgcc
    17401 gccggatcgc tattttactg aaatcaccgt cagccatcgg atggaggttg tgaaagagca
    17461 gattgatttg ctggcgcaaa aagccgactt ctttgtagag cacgacctgc tggcatcggt
    17521 caatattgat ggccctacgc tcatcgccct gcgtcagcaa ccaaaaatcc tgcgccaaat
    17581 tgagcgtctt ccctggctgc gtttcgaact ggtggaacac atccgcctgc cgaaagattc
    17641 aacctttgcc tcgatgtgtg aatttggccc gctgtggctg gatgattttg gcaccgggat
    17701 ggcaaatttc tctgcgctaa gtgaagtgcg ttatgactac atcaaaatcg cgcgagagct
    17761 gttcgtgatg ctgcgtcagt cgccggaagg ccgcacactc ttttctcagc ttttacatct
    17821 aatgaatcgc tattgtcgcg gggtgattgt cgagggcgta gaaacaccgg aagagtggcg
    17881 tgatgttcag aactcgcccg cattcgccgc acaaggctgg tttctttcac gcccggcacc
    17941 gatggaaacg ctgaatacgg cggttctggc gctataagct gcctcatttt ccgctgggct
    18001 ggactatctt taggactggc acaggaagaa tatgaggaag cgaacgatga gcaaggcagg
    18061 caaaataacc gctgcgattt caggggcttt cttgttgttg attgtcgtgg cgatcatttt
    18121 gattgcaact tttgactgga atcgactcaa accgaccatc aaccagaaag tctctgcgga
    18181 gttgaatcgt ccgttcgcta tccgtggcga tctgggcgtg gtgtgggagc ggcaaaaaca
    18241 agaaactggc tggcgcagct gggtgccgtg gcctcatgta catgcagaag acatcattct
    18301 tggtaatcca ccggatattc ccgaagtcac gatggtgcat ttgccgcgag tagaggcaac
    18361 gctggcaccg ctggcgctgc tgaccaaaac ggtctggctg ccgtggttca agctcgaaaa
    18421 gcccgacgcg cgcctgattc gcctctccga aaagaacaat aactggacgt ttaatcttgc
    18481 caacgatgac aacaaagacg cgaatgcaaa gccgtcggca tggtcgtttc ggctggataa
    18541 tattcttttc gatcaagggc ggatcgccat tgatgacaaa gtaagcaaag cggatctgga
    18601 aatttttgtt gatccgttag gcaagccgct gccgttcagc gaagttactg gatcgaaagg
    18661 taaagcggat aaagaaaagg tgggcgatta cgtttttggc ctgaaggcgc agggacgtta
    18721 taacggtgaa ccgctcacgg gtacgggaaa aataggcggt atgctggcgc tgcgtggcga
    18781 agggacgccg tttccggtac aggctgattt ccgttcaggt aatacccgtg ttgcttttga
    18841 tggcgtcgtg aatgacccaa tgaagatggg cggtgtcgat ttacggctta aattttctgg
    18901 cgattcactg ggtgatctct atgaactgac gggcgttctg ctgcccgata ccccgccgtt
    18961 tgaaacggat ggtcggctgg tagcgaaaat cgacactgaa aaatcgtcgg tctttgatta
    19021 tcgcggtttt aatgggcgaa ttggcgatag cgatatccac ggttctctgg tctacaccac
    19081 cggaaagcca cgaccaaaac tggaaggtga tgtcgagtcg cggcaattgc ggctggcgga
    19141 cctgggaccg ttgattggcg ttgattccgg gaaaggggca gaaaagtcga aacggtctga
    19201 acagaagaag ggcgaaaaaa gcgttcagcc tgcgggcaaa gtgctgcctt atgaccgctt
    19261 cgaaaccgat aaatgggacg ttatggatgc cgatgttcgc ttcaaagggc ggcgcattga
    19321 gcatggcagt agcctgccga ttagcgatct ttctactcat atcatcctca aaaatgctga
    19381 cctgcgcctg caaccgctga aatttggcat ggcgggcggc agcattgcga cgaatattca
    19441 tctggaaggc gataaaaagc cgatgcaggg gcgggcagat attcaggctc gtcgactgaa
    19501 actgaaagaa ctgatgcccg atgtggaact gatgcagaag acgctggggg aaatgaacgg
    19561 tgacgcggaa ctacgcggta gcggtaactc ggtggcggcg cttttaggca acagtaacgg
    19621 caacctgaaa ctgttgatga atgacgggct ggtgagccgc aacctgatgg agattgttgg
    19681 gctgaatgtc ggcaactaca ttgtcggtgc gatatttggt gacgatgagg tgcgggtgaa
    19741 ctgcgcggcg gcgaatctga atattgccaa cggcgtggcg cgcccgcaga tttttgcttt
    19801 cgatactgag aacgcgttga ttaatgttac cggcacggca agttttgctt cagaacagct
    19861 ggatttgact attgatccgg agagtaaagg gattcggatt atcacactgc gttcgccgct
    19921 gtatgtgcgt gggacgttta aaaatccgca ggctggggtg aaagccggac cgctgattgc
    19981 ccgtggtgct gttgctgcgg cactggcaac gctggtaaca ccgacggcgg cgttactggc
    20041 actgatctca ccttccgaag gggaggctaa tcagtgtcgg acgatattgt cgcagatgaa
    20101 gaagtgatga taattgccgg atgtctgaca tccggcaatt aaacagattt agatttttgg
    20161 caaagtgtgc tttgttcatg ccggatgcgg cgtgaacgcc ttatccggcc tacaaatcct
    20221 tgtgaattca atatattgta tggaaattca gtcctgataa gcgtagcgca tcaggctttt
    20281 cacacttatt acaacgactg atgtcgcgtc tcatgggtca gcagcagggc gattaacgtc
    20341 aagccagcca tcgccgccag atataacccc accgcaccta acccgtagtt agtctgcaac
    20401 caggctgcga tatacggtgc aacggaagcc ccgagaatcg acgctacgtt gtaagagaat
    20461 gatgctccgg tgtaacgcac ttctgttggg aacagctctg gtaacagcgc gcccatcggc
    20521 ccgaaggtca gccccatcag acttaacccc agcagcagga aggcaaaaac cagaatcggg
    20581 ttgccagaac cgagcagcgg gttaaaggcg aacagcgcga acaggatgat cagcgtggtg
    20641 ataattacca tgcttttacg gcgaccaaag gcatcagcca gtaatccggc gactggcacc
    20701 atcacgccaa aaccaataac tgccatcatc agcatccaca acacttcgtt acgcggcagg
    20761 ccaagcccaa ctggcgcggc ggcggtactg aaggtcatag agtagaccgt catgatgtaa
    20821 aacagcgtat aggttgccag catgatgaac gtacccagta cggttacgcg aacatgtttg
    20881 gtcagcagcg tacccagcgg gatcttcacc tgttttttcg ctttagcgac tttctcaaac
    20941 accggcgact catgcagcga cacgcgaaca tacaggccga taatgaccag taccgccgag
    21001 aagataaatg gcacacgcca gccccagctc ataaactgct cgtcggtcag tagccaggaa
    21061 agcagcaaga aagtgccgtt ggcaaagaag aagccaatcg gtgcgcccag ctgtgggaag
    21121 gagccataca gtgcacgttt gcgcggtggg gcgttttcag tcgccagcag cgccgcgccg
    21181 ccccattcac cgcctaagcc cagaccctga ccaaatcgag ccagcgccag cagcagcggg
    21241 gcgaaaatac caatcgtggc atagcccggc agcagaccaa tcaccacggt tgaaatcccc
    21301 atcgttagca acgaggcgac cagcgtcgct ttacgcccaa cgcgatcgcc aaaatgacca
    21361 aaaacggcag agccaatggg gcgcgcgacg aaggcgatgg cgaaggtggc gagcgactgt
    21421 agcgttgctg ccgttggatc gccctgcggg aagaagatat gcggaaacac aataacggcc
    21481 gcagtggcgt aaatgtaaaa gtcgaagaac tcaatggccg tgccaatgag agaggcgaca
    21541 aggactttat tacgcgagtt gatcggcgtg tattcttgct cgtggtcgag tgttgtggct
    21601 gttgcttgca taatcttttc ttattttttg gctaacgaat agccatatta cgcacagcaa
    21661 aagtgatatt tcaatgtgta acaaatcgcg gaaatggtca aaaaccaggc aaaaagggga
    21721 taatgtttag gccagtgatt tttcttctat gaaatgcttc acataatttt ataaatagtg
    21781 gataaaactg gtttcaggtt aaataaaagt taccaactgg atttatatgc tggggttatg
    21841 agttggaatg aaattgtttg ctcccaaata gatggctgaa gaggctctgg ggaagagttt
    21901 caagaggtta tgtgcgcatt atagccggat gcgatgctgg cgcatcttat ccggcctacg
    21961 gggagccagg ccgaataaaa tcgtatgccg gataaggcgt ttacgccgca tccggcctat
    22021 tttaaggctg cgttttccct ggcattcgcg ggtcgtcttt atattcggcg gtggcaatcc
    22081 acgccgcgca aaacagcgtc aaacgggcga agaagtagaa aaatgccatc agccccagca
    22141 cggaaccaaa tgctgcgcca gagggggatt tcatcagcga tggcagggtg taggtcatca
    22201 cgattttaat cacttcaaaa ccaatagcag ccagaaatgt tccgcgaatc agggcttttt
    22261 tgcgcggacg gtggcgcggt aaccgccaga agatccagaa gaaaagcaga tagttggcga
    22321 agatggaaat cgccaggcca atcaaccgcc acgtcggttt cagccactca atgctgttca
    22381 ggtgcagggc gctaataatc atttgctgcg ccgaaccggc aaccgaggtg atcgaaagcg
    22441 tcacaatcag cgcaatcaac aaaccaatca gcgaaataaa atcacgtaga tattttaccc
    22501 agaacttctc ctgatcttgc ggcgagcgtt cccagacatc gcgcgactgg gcgcgaatcg
    22561 cttcacgcag gttacccatc cagttgatgc cggaataaag cgccacagcc aggccgacaa
    22621 gccctacagt cgtacgctgc tgaacggcgg tgttgatggt gtttttcaac gtggcggcta
    22681 gcgtcggatc gctgatgttt tgcagaattt tgtcgaagat atcctgtagc aacatcggat
    22741 gggaggccag cacaaagccc cctgcggcaa acgacaccat caaaatcggg atcatcgaca
    22801 aaaatgagaa ataggtgata gccgcaccaa actggttacc cagccgatcg ttaaagcgtt
    22861 ctgtcgcgcg aatcaggtgc gcaataaccg gttggcgttg gactttttcg gcagtggtgg
    22921 tgacggtttc cagcgcctgg ctaactttgc cgcctttctc gctattatcc aactgcttaa
    22981 tcggctcgtg ctccagatcc tgggtgggac gtttgatctc gttttcctgc gtcatccgct
    23041 cttttgtcct tctgttgtgg tcattcttaa aaagtatagt cggtcagtcc aggtactctt
    23101 tcattacgcc agccagccac tgcataaaca ggtttacacg ccgggaaagc tcccgacgct
    23161 gcggataaac cagcgaaagg gagagcggct cggcacggta gcctggcaac acttcaataa
    23221 gctgcccggc acgcagggct tcgcgcacgg caatgcgcgg gatctgaata atccccagac
    23281 cggcaagacc ggcggcgaga tagttttcgc tactgtttac cgtcaacatg ccgccagact
    23341 taaaccactg gacgccatta acgctggcaa cctcaaaacc taacggatgt acacccaggt
    23401 gcggtgtata acgcaccatt gcgtgtgaag tcagatcatc ggggctttgc ggataaccaa
    23461 agcgcgtcag atagtgcgga ctggcacagt tgaccatggt cagtttgccg aggggacggg
    23521 cgataacgcc atcttccggt aacgcgccag tgcgtatcac gcaatcaaaa ccatcatgaa
    23581 gaatatctac cggacggtta tggctactca gttccagctc aattcccgga tgctgataga
    23641 gaaattccga cagacgcggc agtaacaggt ttttcgcgat tcctgacggg atgtcgatgc
    23701 gtaatttacc gctgatactg gtggcatcct gttgaaacag accgtccagt tcgctgagat
    23761 tactcaacac atcttttgcc cgttgataat aggtcatgcc ttctggcgtg agtttgaccc
    23821 gtcgcgtagt gcgctggaga agctgggtgc caagttgatg ttccagtgcc tgtatctggc
    23881 gcgaaacact tccctttggc aaagcaaaga aatccgccgc gcgggaaaaa ctttccagct
    23941 ccgcgacttt gatgaacaac tgcattgcgt gaattttatc catttctttg accgattgtt
    24001 gttcacagcg aaacagtaac gcgcatatcg cattgtttat tgttttttct aacatctaat
    24061 gccgggtttc acaatgttgt catgacgaat gtggcgattt ctcagcagga aaagggcgga
    24121 atagctttat gagaaaggtg aatcccctta aacagagcgt atttttcagt gattcccttt
    24181 aaagtgaata gaggacgtat tggctatttg tgcttatttt tgtctcattg ccatttgtta
    24241 ttttggcttg tagtcgcgga atttcatttt taattgcatg cgtactaacc accaatataa
    24301 ggaaatcctc aaaattgttt taaatagtgc cctcgacatg taatttcagg cgtttagctg
    24361 taactattta tatcggtaac tattttctac gcgtcatctc tgtagaatgg ccaccgccaa
    24421 atggcgaatt cttatttttt ctcgcgggta atacccttcc actacttaag tctttttaac
    24481 gtattcaggt ggagattcag ttatgcaaat agtcatgttt gacaggcagt caatatttat
    24541 tcatggaatg aaaatcagtt tacagcagcg tattccagga gtgagtattc agggggccag
    24601 tcaggcagac gagttatggc aaaagcagga aagttaccct gaagccttag ttatgctcga
    24661 tggcgatcag gatggtgagt tttgctactg gttgctgcaa aaaaccgtgg tgcagtttcc
    24721 tgaggttaag gtgttaatca cggcgacgga ttgcaataaa cggtggttac aggaagttat
    24781 tcattttaat gtgctggcca ttgtgcctcg tgattcaacc gtcgagacat ttgcgttggc
    24841 ggtaaatagt gcggcgatgg ggatgatgtt tctgcctgga gactggcgga cgacgccgga
    24901 aaaggacatt aaagatctca aatcgttaag cgcccgccag cgggagattt taaccatgtt
    24961 agcggcgggc gaatcaaata aggagatcgg cagagcgctg aatatcagta ccggaacggt
    25021 aaaagcgcat ctggaatccc tgtatcgccg tctggaagtg aaaaatcgca cccaggcggc
    25081 aatgatgtta aatatctcct cctg
//
LOCUS       DAEEKQ010000050        24180 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.050, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000050 DAEEKQ010000000
VERSION     DAEEKQ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.050"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            67..228
                     /locus_tag="KLK57_001912"
     CDS             67..228
                     /locus_tag="KLK57_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892287.1"
                     /translation="MEFSFSPKLLVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(312..1547)
                     /locus_tag="KLK57_001913"
     CDS             complement(312..1547)
                     /locus_tag="KLK57_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBE0892288.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAARDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1694..1770)
                     /locus_tag="KLK57_001914"
     tRNA            complement(1694..1770)
                     /locus_tag="KLK57_001914"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1734..1736),aa:Pro,seq:tgg)
     gene            complement(1813..1899)
                     /locus_tag="KLK57_001915"
     tRNA            complement(1813..1899)
                     /locus_tag="KLK57_001915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1863..1865),aa:Leu,seq:cag)
     gene            complement(1920..1995)
                     /locus_tag="KLK57_001916"
     tRNA            complement(1920..1995)
                     /locus_tag="KLK57_001916"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1960..1962),aa:His,seq:gtg)
     gene            complement(2054..2130)
                     /locus_tag="KLK57_001917"
     tRNA            complement(2054..2130)
                     /locus_tag="KLK57_001917"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2094..2096),aa:Arg,seq:ccg)
     gene            complement(2231..3616)
                     /locus_tag="KLK57_001918"
     CDS             complement(2231..3616)
                     /locus_tag="KLK57_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0892289.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3807..4547)
                     /gene="rffM"
                     /locus_tag="KLK57_001919"
     CDS             complement(3807..4547)
                     /gene="rffM"
                     /locus_tag="KLK57_001919"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HBE0892290.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4550..5902)
                     /gene="wzyE"
                     /locus_tag="KLK57_001920"
     CDS             complement(4550..5902)
                     /gene="wzyE"
                     /locus_tag="KLK57_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HBE0892291.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNREPNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5899..6978)
                     /gene="rffT"
                     /locus_tag="KLK57_001921"
                     /gene_synonym="wecF"
     CDS             complement(5899..6978)
                     /gene="rffT"
                     /locus_tag="KLK57_001921"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HBE0892292.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6975..8225)
                     /gene="wzxE"
                     /locus_tag="KLK57_001922"
     CDS             complement(6975..8225)
                     /gene="wzxE"
                     /locus_tag="KLK57_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HBE0892293.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8227..9357)
                     /gene="rffA"
                     /locus_tag="KLK57_001923"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8227..9357)
                     /gene="rffA"
                     /locus_tag="KLK57_001923"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HBE0892294.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9362..10036)
                     /gene="rffC"
                     /locus_tag="KLK57_001924"
                     /gene_synonym="wecD"
     CDS             complement(9362..10036)
                     /gene="rffC"
                     /locus_tag="KLK57_001924"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HBE0892295.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAQLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGGIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10014..10895)
                     /gene="rfbA"
                     /locus_tag="KLK57_001925"
     CDS             complement(10014..10895)
                     /gene="rfbA"
                     /locus_tag="KLK57_001925"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HBE0892296.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     DIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10914..11981)
                     /gene="rffG"
                     /locus_tag="KLK57_001926"
     CDS             complement(10914..11981)
                     /gene="rffG"
                     /locus_tag="KLK57_001926"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HBE0892297.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVVHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11981..13243)
                     /gene="wecC"
                     /locus_tag="KLK57_001927"
     CDS             complement(11981..13243)
                     /gene="wecC"
                     /locus_tag="KLK57_001927"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HBE0892298.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEVLATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13240..14370)
                     /gene="wecB"
                     /locus_tag="KLK57_001928"
     CDS             complement(13240..14370)
                     /gene="wecB"
                     /locus_tag="KLK57_001928"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HBE0892299.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANDPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14426..15472)
                     /gene="wzzE"
                     /locus_tag="KLK57_001929"
     CDS             complement(14426..15472)
                     /gene="wzzE"
                     /locus_tag="KLK57_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HBE0892300.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15484..16587)
                     /gene="wecA"
                     /locus_tag="KLK57_001930"
     CDS             complement(15484..16587)
                     /gene="wecA"
                     /locus_tag="KLK57_001930"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HBE0892301.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16827..18086)
                     /gene="rho"
                     /locus_tag="KLK57_001931"
     CDS             complement(16827..18086)
                     /gene="rho"
                     /locus_tag="KLK57_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HBE0892302.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18171..18272)
                     /gene="rhoL"
                     /locus_tag="KLK57_001932"
     CDS             complement(18171..18272)
                     /gene="rhoL"
                     /locus_tag="KLK57_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HBE0892303.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18413..18742)
                     /gene="trxA"
                     /locus_tag="KLK57_001933"
     CDS             complement(18413..18742)
                     /gene="trxA"
                     /locus_tag="KLK57_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HBE0892304.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18873..20138
                     /gene="rhlB"
                     /locus_tag="KLK57_001934"
     CDS             18873..20138
                     /gene="rhlB"
                     /locus_tag="KLK57_001934"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HBE0892305.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20272..21756
                     /gene="gppA"
                     /locus_tag="KLK57_001935"
     CDS             20272..21756
                     /gene="gppA"
                     /locus_tag="KLK57_001935"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HBE0892306.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIANAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVEKEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21803..23824)
                     /gene="rep"
                     /locus_tag="KLK57_001936"
     CDS             complement(21803..23824)
                     /gene="rep"
                     /locus_tag="KLK57_001936"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HBE0892307.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
BASE COUNT         6071 a         6636 c         5984 g         5489 t
ORIGIN      
        1 agggatctca attatcaatg cttaattacg tcatcatttt gatgacatga aaccggagca
       61 ttttgcatgg aattttcgtt ttcacccaaa cttcttgttg ttgctgtcgc cgccgctctt
      121 cctctcatgg ccagcgcagc agataccccg tcaactgcca ccgcacgcaa aggctttgcc
      181 ggatacgatc acccaaacca gtatccacag aacgctgcaa tttattgaat ttgcacgatc
      241 atgtaggccg gataaggcgt ttacgccgca tccggcaatc aaccgcaggc ggccacaaaa
      301 tggccgcttt tttacttatt caccaccagc aaatgtgcct gcataatgtc actggccggg
      361 cgaccgtgcg ccagcaaatc agccattgct ttaagatatg gcggtagatg gcggaaataa
      421 cgctgatacc cggcacacaa ataattcagc cccggtttgc cgctggcatc gagcatgaag
      481 cggtgtttcg ggcagcctcc ccagcacgct tttaacacgt tacaactgcg acactgcgcc
      541 ggtaattgct taaatttatc ttcaccaaac acctgctgtt gcggggaatc gatcatttct
      601 gcaattgttt gctgatgcat attccccagc cgatattgcg gataaacata gtgatcgcag
      661 gcgtaaacgt cgccgttgtg ctcaacaatc accgagcgcc cacaggttgg ctgatgatgg
      721 caaaccgcac ccggcgcacc gacaaaattg gcaaacgccc attcgatatt catcacgaaa
      781 atcttgccga cgtcgcgttt gatccagtgg tcgaatatcg ccaccagaaa ttcaccaaat
      841 tcatcggggc gcaccgacca ttccgtcagt tccccctgaa tatcaccagg cgcatgcaat
      901 ttcagtccat cgcgggcagc tgtttcatca gccagacgct cgaccaccgg aataaactgg
      961 atgaattcga ctcccgcatc gcacaaaaaa tcataaacct gcaacggttg ctgcgcgctg
     1021 gtgcggttga cgcagaccag cacgttatag tcgacatgat gtttttgcag cagcgtcagg
     1081 gcacgcatca ccagcttatg cgttggtctg ccacctttgg tcacgcgata ttgattgtgg
     1141 atctcagccg gaccgtccag cgataaccca acaagaaaat gattttctgc cagaaatgca
     1201 caccatttat catcgagcag cacgccgtta gtctggaagc tgttacttat cttcctgcca
     1261 gcaccgtatt tcgcctgtaa ctttacggca cggcggtaaa aatccagccc gagtaacgtt
     1321 ggttcgccgc cctgccaggt aaaagccact tcgttttgcg gttcgctggc agcgatatag
     1381 tgacggacat acgcttccag cgtgtcatcg tccatatgcg tgactggctt ttcgcggtaa
     1441 agggattgtt tttcgagata aaaacagtag tcacagttca gattacaatc ggaaccactc
     1501 ggtttcgcca tcacatgaaa agcacgcgtt ggaacctgtt gcagcatggt cactccttgc
     1561 ctgaagtcat ccttcaatac gttaagggcg aaatcatacc gtggggcaat gagttattac
     1621 gggcgtttat tgcgaaggga gggtgacgaa atgcacagaa aacaaaaaac cccgccgaag
     1681 cggggttcaa aattggtcgg cgagagagga ttcgaacctc cgacccactg gtcccaaacc
     1741 agttgcgcta ccaagctgcg ctactcgccg aaatactgct ttttgaattt ttagttcaat
     1801 tctttaaagt cgtggtgcga ggggggggac ttgaaccccc acgtccgtaa ggacactaac
     1861 acctgaagct agcgcgtcta ccaattccgc caccttcgca ttgtcacaac ttctaataat
     1921 ggggtggcta atgggattcg aacccacgac aactggaatc acaatccagg gctctaccaa
     1981 ctgagctata gccaccacta caaatcttgt tacgcggtat tactaccacc gcagctcaag
     2041 cgccgggact aaatggcgcg cccgacagga ttcgaacctg agacctctgc ctccggaggg
     2101 cagcgctcta tccagctgag ctacgggcgc ttagcgccgt tgcgggggga taatacggac
     2161 ttcccacccc tctgtctagt ccctttttaa ataaattgcg cgtttggtta cggtttgtgc
     2221 gttttgctgc ttattcctcc agtttatgcg ctttcccgtg gcgattaagg ccaaaaactt
     2281 tataaatcgc cgtcaccgcc agcataaaga tgatgccaac aaacagcgac atacgcgtat
     2341 cttcattaaa gtacatgccg atcaaaacgc aaatcaggaa tgccattgtt acgtagttgg
     2401 cccacgggaa caggattgag cggaacggat ggctggcaat cgccgcttta tgtgcacgcc
     2461 gaaaacgcag ctggcttatc aatatcacaa accatggcac catccccgga agcacgctgg
     2521 cactgtagac gtagacaaac acacgctgcg gattgggaat gatgtagttc aggcatgagc
     2581 caattagcag aatagcaata gatactgcca cacccgcaac tggtacgccg tgacgggaaa
     2641 ctttcgccat cgccgccggt aactgacggt ttttcgccag tgcgtagagc atacgtccgc
     2701 aactgtacat gccgctgtta cagccagaga gcgcagccgt cagcaccaca aagttgataa
     2761 tgcccgccgc tgcggtaata ccgattttgg caaaagtcag tacgaacggg ctgccgttgc
     2821 tgccaatttc attccacggg aagatggtga cgataacgaa aatcgcgcct acgtagaaaa
     2881 tcaggatccg ccacagcact ttgcctacag cactgcgcag cgtcacctgc ggattcttcg
     2941 cttcaccggc agtaatgcca atcagctcca cgccctggta ggacgccacc acaatacaca
     3001 gcgcggtcag gaaccctttc cagccacccg caaagaaacc gccatgctct gtgagattgc
     3061 taaaaccaat cgactgcccg ccattgccaa agccaaagaa aatcacgccc aggccaatga
     3121 caatcatcac gataatcgtg gtgactttga tcatcgcgaa ccagaactcg atttcgccgt
     3181 acaaccgcac cgccgccaga ttcgccaacg ccaccagcgc caccgcgatc aatgcaggta
     3241 tccactgcgc catctccggg aaccagaact ggacataaac gccaatggcg gtgatttctg
     3301 agatccccac cgccatccac ataaaccagt aagaccaggc ggtgagataa ccaaaaaacg
     3361 ggctcatata acgatgcgca taaacggcga acgaaccggt aacaggttca aggaacaaca
     3421 tttcgcccat tgaacgcatg atgaaaaaga cgaacagccc ggcgatgata taggccaaca
     3481 atacggatgg cccggcccat ttcagggtac tggcggcccc cataaacagg ccgacgccaa
     3541 tagtgccccc cagggcgatg agttcgatat gtcgagcttc cagcccacgc tgtagctctg
     3601 gtttgttatc tgccataaat cctcgtgttg tgtttgcatg ctttccggtg ttaccggtta
     3661 tcgttatggg tacatcgagt gttgcgaatg ttttcgtaat tcaggagaaa tggcaaataa
     3721 agcattaaaa atttgaatgc tttgtgtaat aaaaaagcag acaggcgacg gagtgaccac
     3781 tccgtcgctt tacaaagaga ggaaaatcag aggttgccgg tgtagtgcca gcgtaaataa
     3841 cgcagcaaac gaagctgacg cttaatgcgg ctcggctgcg aaagcaggcg gtagagccac
     3901 tccagtccca gcgtttgcca gattttcggt gcgcgtttta cgtgaccggt gaaaacatcg
     3961 taagtcccgc caacgcccat atacagcgca tctggatgca ccagacggca gtcgcgcatg
     4021 aagatctcct gctttggcga tcccatcgca acggtgacga tttgcgctcc gctggcatga
     4081 atgcgttcaa acagcgcctg acgctgctcg ggtttaaaat aaccatcctg actgccaacg
     4141 atattcacat tccattggtt gcgcagttta gcttcagttt gcgccagcac ttcaggttta
     4201 ccgcccacaa gaaataccgg cgtcccttct ttgcctgcgc gcgccatcag ctcttcccag
     4261 agatcggcac cggcaacgcg ggaaacctgc gcctgcgggt actttttacg tactgaacgt
     4321 acaacgctga tgccatccgc atatttaaat tcggcagcgt taattaactc cctgacctcg
     4381 gcgttatctt caatggtcag cattttttca gcattaatgg caaccagcgt tccctgctta
     4441 agctgcccgt cagcaaacag ataatcgagg gcgtgctgca tatcacgcca accaatcaac
     4501 tgtaagccac gcagcgtata ggtcggtgcc gtggtgttgt tattcattgt tatccttcaa
     4561 cctgcgtccg gagcgatgat tttgtacgtt tatgaatgag tccggcgctt tcaaaaagcc
     4621 agtacagcag ttttgcgatc atcagacatg cgccgaagac cacgataaaa aagactacgc
     4681 gtgagacaaa cgaatccagc ccttcacgcg ccagcacgat catattgaag atcgccccaa
     4741 agcagaaact gtgcaatatc gcagccttat agcgattagg ctcgcggttg cccagctcat
     4801 acagccagtc gaaccatttg atgatcagac caaccacgat cgccccgagc gggatgaaca
     4861 acgcgccgcc catcaccacc agtgagccta taagcgtagg cgagatcgcc agtccggagt
     4921 ggttattcag cacttcccag gtaaagtagt tggctgagtt cagcaccata ctcgggcgac
     4981 ccggccacag ccaggaaggg ataaagacat agaaatcgcg gacaattgga gccaggccct
     5041 ggaagtcgat gttgtcgtag ttctgcaaca gcaacgccag attctcccac ggggagaagg
     5101 tgtcgcgagt gagatagaga aacgtataga acgcttcatc gccgctcaca ttcattccat
     5161 agcgttttag cgccagccag aacatgccaa caatccccag cacgcccgcc gccgccagca
     5221 tccacaacga aatccagccg cgaataatgc caataaacag gaagatagcg aatgcgatga
     5281 tgatattggc gcgagtgccg ccgacaatca tataagtcag caagccaaag gcgaccgtgc
     5341 tgacgaggaa aaacagccac gctttgctgt cctggcgcag aaagtagacc accagcatcg
     5401 ccgggatgaa aaagtaaaag aagcgtttta acgccacgcc ggagacttca ctggagaaga
     5461 tctgactgta ggagttaagc cggaacagca aaaagccgtt gtgcatgaag aagatgccga
     5521 cgcttaccag cgcgataccc atcaggatca cccacgtaag attggtctcc acgcggttca
     5581 tggtaaacag cggacggcgc ggtacatcag caacgcgttt gcgtaggcgg gttttgtagg
     5641 tgacatagta aaccgcgtag aagcagcccg cagaaagcaa cgcctgcaac aagatttctg
     5701 gcggcgcgac accaacatca aagcgaaata ccagcacgct ggtcagcggg aagccgaaga
     5761 aaaaggtgag caaaaacagc aatgaaaaga agacattgaa gttaaagcgc acacggcgaa
     5821 actcaaacca ggtcagcgtg gcaataaaca gcgtgcagag cagccagacc acaaacaggc
     5881 cactgaattg cagcagactc atgcgacctc cccggcggca atagccaacg cccgctgcca
     5941 gccttgtaga tagttagggc taaagaaggc aatggtgttt ttatccaccg acgccaactg
     6001 gcgctgcgct tcacgcacaa tatcctcgtt gagatcgtcg gtagtaaaca gcaccggcaa
     6061 atgctgttcc gtcatatcct gccagaacgg attttcccgg ttaagcacac aaggaatgcc
     6121 cgcctgaatc agtaagcaaa gcgtaccaat gccctgctgg cgggcaaaaa taaagtaacc
     6181 aagatcgcac tgacgaagta gcgccagata ggcgtcaaat tccagttttt cgctcaaaat
     6241 ttgcagattt tcttcgctga ataactccag ccccgcctga cgaacttcct caatgtacgc
     6301 ttcgttatta ggcggatatc ccatcggcac caccactttt accgtatcgc caaattgctg
     6361 atgaacggcg cgcaaggcag caatatggtc attgctgcgg tcaccggagt tccccaccag
     6421 aatggtcatt ttcccttcac gttgccgatc attcgccatc gtattgagag aagggtccat
     6481 ccgcgtcggg aagtacagca gttcgccccg cacctttgga tgcgttttgg caaaaaagct
     6541 caaatcaccg cgggtggcaa atacacagcc gactcgcttt tgcgccaggc gacgtagcgg
     6601 gtaaaaaagc ttatatctca agccactgga aagctcatac aggtctgccc cccagatatg
     6661 ccagaaaaac tggctgggct taatgccacc actcagcaga gccagccaca gtgtgggatt
     6721 gaactgaccg tggaaaaaaa atcgctgctg acggttagct tttgctttcg cgatgaccgc
     6781 ttccgccagc gattttttcc cagggaaaaa ttgcacagaa agcgccggac agctatcact
     6841 taagccgtcg tccttgccaa caaccataaa ctcgcgcgcg tgctcgctcg tcgcggccag
     6901 cgcgtcattg aaaaaccgca aaacggttcg gttatggtga gggatatccg atcccagtac
     6961 gtgaatcagt acagtcatgc ccgcctacgc cagagtaaaa acacgccaca acaaagagaa
     7021 aaatagacga tataagttgc catatatgcc tgcgccgcgc ccagtgcacc atgcgcaggg
     7081 attagccagt gggcaaatac catcaataaa gtgaactggc tgacttccgc cagaatataa
     7141 aaccgcagtg acgctttggc gatcaccaga taaccaaaga cataagcgcc cacttttaac
     7201 acatcaccca ctaactgcca ggcaaagaga tcgcgcatag cggtaaattt attcgacaac
     7261 agcaaccaga tagcaaaatc acgcagcaac cagacggtaa aactcgccgc cgccactgcc
     7321 ggtaagacga atttcagcga tttaaccact tcccgggtga tatcgcgctt ttccgttagc
     7381 cgcgacaacg tgggcagcaa atatacgctg aacgatgccg taataaattg caggtaggca
     7441 tcggaaatac tgctcacccc ttgccagatc cccacttcat cccagctata ctgcgccgcc
     7501 agcagtttac gcatcatgat gtaagcaaca ggcaaggtca ccgacgtaat caacgccatg
     7561 agcgtaaatt tgctcaactg ccctgccaga ccgttatccc agctgggttt cagatagctt
     7621 aacgggatga caccacgttt gatcaacatg atggcggcag gaatcaccac cagcgcggga
     7681 atcagcgcca gacccagcaa cgccccttca taaccgccca aacggtaact gacgtagtac
     7741 gcgagaacgc caatcaagct gccgacaatc agcgataacg cattacctgc ggcatcgcga
     7801 aagcctttca tcagcgccag taacaggttg ccccaggcga tccccatttg caccagcgcc
     7861 actaaacgca ccagcccctg atagtcggta ttaccaaaca gtccctggct gattggcgca
     7921 gctgccagca caaaaaccag cgccatcagc gtagagaaac caagtaccat cgctgatgaa
     7981 gtgccgacca cgcggcgcag ctgttgcgga ttatcatggt actgggcaac gtatttggtt
     8041 acaccgttaa agatgccagc tccggcaagc acgccgagca cggtaatcaa ctggcggaaa
     8101 tttgccgcca gcccaagccc cgccggacca aatgacacag ccagcaactt accgaccagt
     8161 aacccggcac caatcttgac cagtgtactg gccgccgtcc acaaggacgc ttttgccaac
     8221 gacatatcag gagaagtagt tcaacaaagt cgcaattacc gtacgctgat tgacgggcga
     8281 caggttgtag aacagcggca ggcgcagcag gcgctcgctc tctttggtgg tgtagcgatc
     8341 ttcaccgtgg aactcaccaa agcgttcccc cgcagggcaa ccgtgcagcg gaatgtaatg
     8401 gaacaccgcc atgatttccg cttctttcag aaagttaatc aacgcgctcc ggtcatcaat
     8461 atcccgcagt ttaatgtaga acatatgcgc gttctgcacg cagccatcgg gaatcgacgg
     8521 cagctcgata cgcccggctt tcgccagagg cgctaacgca tcgtagtagt tttgccacag
     8581 cgccagacgt tgctggttga tacgatccgc tgcttccagt tgcgcccaca ggtacgcagc
     8641 ttgcagatcg gacatcaaat agctggagcc aatatcgcgc caggtatatt tatcgacctg
     8701 accacggaag aactggctgc ggttagtgcc cttttcacgg atgatctcgg ctcgttcgat
     8761 taacgcttta tcgttaatca gcgtcgcgcc gccttcacca cccgccgtat agtttttggt
     8821 ttcatggaag ctaaagcagc caatatgacc aatggttccc agtgcacgcc ctttgtaagt
     8881 ggacatcacg ccctgagcgg catcttctac cacaaacaga ttatgctttt tcgccaacgc
     8941 cataatggtg tccatttcgc aggccacacc cgcgtaatgg accggcacga taacgcgcgt
     9001 tttgtcggtg atcgccgctt caatcagcgt ttcgtcgatg ttcatggtgt ccgggcgaac
     9061 atccacaaaa acgatttttg cgccacgcag cacaaaggca ttggcggtgg agacaaaggt
     9121 gtagctcggc atgatcactt catcgccagg ctggatatcg agcagcagcg ccgccatctc
     9181 cagcgaagcg gtgcaggacg gcgtcagtaa cactttggcg ctgccaaaac gttgctccag
     9241 ccactgctgg cagcgacggg taaaaccgcc atcgccacac agtttgccgc tacccattgc
     9301 cgactgcata tagtcgagtt cggttcccac caccggcggt gcgttaaatg gaatcatgtg
     9361 atcacctgta taaccagtac gcggtgcttt ctacattcgc accactttgt atgtatcgtt
     9421 taagcgcggc ggtgttgccc atttgggtcg ccacccgcaa agttgtttta ccgcgagcat
     9481 acgcccagtt tagcgccgtt tgcatcagct cagcacctgc accgcgtcca gccagcaggc
     9541 caattcgcgc atctgtcgca ttgagttccc gtaaagagac ataaccgcga ataccgccgg
     9601 acgccgcacg taaaatcaga cattgatgat caaaggtgcc gcgcacggca ttttcaatcc
     9661 actgtgcata aaagcgtccg ctggcgtcag gcgcatacca cggcgcacga aaacggcttt
     9721 gcgcaaatgc ggcgctggct aactgacgta atgcgggaat atcggtctct tgtgccacta
     9781 cagcaccact atcactgaca ttgttcacgg gtagtgccaa atcaacttca ccttctacca
     9841 gggagaatcc cagctgttgc agggcatcca gttcacccgt atttgatgcc gcaattttgg
     9901 cctgcacccg tgaccacggc gctaacacgt ctggcgtcag gagctgtgct tcagacgtaa
     9961 tgcgcacgat ggcgctgtta acaccaaaga aggcgttttc ccaggttagt ggctcaatac
    10021 tggcgcggac gggcacgaag taactccagc agatattggc cgtagccagt tttcgctaat
    10081 gaactggcag cacgcttcac accctcgtca tcgagccagc cgttacgcca ggcgatctct
    10141 tccaggcagg caatcttaaa gccctggcgt ttttctactg tctgcacaaa ggtactggct
    10201 tcaatcaggc tgtcgtgagt gccagtgtcc agccaggcaa atccgcgccc gagcagttca
    10261 acggtcaggt tgcccgcctc gaggtacatc tggttgatgg aggtaatctc cagttcacca
    10321 cgctccgacg gcttcacctg ctttgcgtac tccacgactt tactgtcgta gaaataaagc
    10381 ccggtcaccg cccagtttga cttcggctgt tttggctttt cttccagcga gatagcgcgg
    10441 aaattgtcgt caaactccac cacgccaaag cgttccgggt ccatcacctg atagccaaat
    10501 accgtcgccc cttccgtgcg cgccgcaaca tgacgcagct tcggactgaa gccctgaccg
    10561 aagaagatgt tatcgcccag caccagacaa gaaggttcac cgttgaggaa ggtttcaccg
    10621 atgataaagg cctgcgccag accgtccggg ctgggctgtt cggcgtattc cagctggata
    10681 ccgaactcac taccatcgcc cagcaggcgc tggaaataac ctttatcttc cggcgtagtg
    10741 atgatgagaa tttcgcggat atcggccaac atcagcaccg acagcggata gtaaatcatt
    10801 ggcttatcgt aaatcggcaa cagttgcttc gatacgccgc gcgtaatcgg atgcaatcgg
    10861 gtgccggaac cgcccgccag gataatacct ttcatgtttg cctccgctga aaattagcct
    10921 ttcagaccta aacgctcgcc ctgatagctg ccgtcctgca cctgcttcca ccagctttca
    10981 ttagccagat accactgcac cgttttacgc attccacttt caaaggtttc ctgtggcagc
    11041 cagccaagtt cacgggcaat tttcgaagca tcaatggcat aacgcagatc atgccccgga
    11101 cggtcagcga caaaggtgat caagtcacga tagtgcacca cgccgtgcgg cttgttcgga
    11161 gccagttctt ccagcagctc gcaaatggtt tccacaacat cgagattctt acgctcgttg
    11221 tgaccaccaa tattataggt ttcaccgact ttcccggtgg tcgccacgca atacagcgcg
    11281 cgggcgtgat cttccacata cagccagtca cggatttgct gcccgttgcc atataccggc
    11341 agcggtttac ccgccagcgc gttgaggatc atcagcggga tcagtttttc cggaaagtgg
    11401 taaggaccgt agttattcga gcagttggtg ataagcgtcg gcagaccgta ggtccgcagc
    11461 caggcgcgca ccagatggtc gctgctggct tttgacgcgg aataagggct gctcggcgca
    11521 tacggcgtgg tttcggtgaa gaaatcatcc gtcgagtgca ggtcaccata tacttcgtcg
    11581 gtggagatat gatgaaaacg gaacgctgat tttttatctt ccgtcagcgt attccagtaa
    11641 gcccgcgccg cttcaagcaa tgtataagtc ccgacaatgt tggtttcaat aaacgctgcc
    11701 gggccgtcaa tagaacggtc aacatggctt tcggctgcca gatgcatgac acagtctggc
    11761 tgatgctcag tgaatacgcg tgccagttct gcccgatcgc agatatcaac tttctcaaag
    11821 gcaaagcgct cgctttgcgc gaccggtgcc agcgacatca ggtttccggc gtaggtcagc
    11881 ttatcgacca ctaccaccgc gtcgctcgtt tcgttgatga tataacgcac cagcgccgag
    11941 ccaataaacc cggcaccacc tgttatcaga atttttctca tcagcgccag actcctttgg
    12001 catcgacgac atactgctga tggacattgt cgccattgat aactttaaac tgactatgat
    12061 cgaccagcat caccagcacg tctgccgttg ccagcacctc gtcaagctgc gccagagtac
    12121 aaagcccggt cagttttttc ggcaactggt ggatgttagg ctcaacaacc agagtttcgc
    12181 cgctatgcca ctgggcgatc agttcagcga tttccatcgc cgggctttcg cgcaggtcat
    12241 caatattcgg tttaaacgcc agaccaaagc aggcgatttt cagttcactg gcgcgtttat
    12301 cggtagccgc caggcaatca gccaccgccg ctttcacctg atcgataacc cagaacggtt
    12361 tgtgatcgtt cacttcgcgc gcggtacgga taagccgcgc ctgctggggg ttctgtgcca
    12421 cgataaacca cggatcaaca gcaatgcagt gaccgcccac gccagggcca ggctgaagaa
    12481 tattaacgcg agggtgacga ttcgccaggc gaatcagttc ccagacgtta atcccctgat
    12541 cggcacaaat cagcgacaat tcattagcaa aagcgatatt cacatcgcgg aagctgtttt
    12601 cggtgagctt acacatttcc gccgtccgcg agttagtgac gacacactca ccttcgagga
    12661 aaattttgta cagttcgctg gcgcgggtcg aacaaaccgg cgtcatacca ccaatcacgc
    12721 gatcgttttt aatcagctcg accataacct ggcccggtaa cacgcgttcc gggcagtaag
    12781 caatgttgac gtccgcctgc tcgcccacct gctgtgggaa agtgagatcc ggacgcatct
    12841 ctgctaacca ttctgccatc ttctcggttg accccaccgg cgaggtggat tcaaggatca
    12901 ccagcgcgcc ttttttcagc actggcgcaa tggagcgagc agccgattca acgtaggtca
    12961 tatctggctc atgatcgcct ttaaacggcg tgggtacagc aatcagccag gcatccgctt
    13021 caactggcgt cgtgctcgct cgtaaaaaac cgccttctac ggcagttttt actacgctcg
    13081 ccaaatcagg ctcgacgata tggatttcgc cacgattgat ggtatcaacc gcgtgttggt
    13141 tgatatcgac accaatcacc tgtttttgcc gtgaggcaaa cgctgctgct gtaggcagcc
    13201 cgatgtaacc cagtccgata acagaaatgg tcgcaaaact catagtgata tccgattatt
    13261 ttttaacgct tccagaatgc gagagcatgc ctgaccatca ccatacgggt tatgggcgcg
    13321 gctcatagtt tgatattcgt tttcgtcttt taaaagacgc gtcacttcct cgacaattcg
    13381 ctgcttatcc gtgcctacca gacgcaccgt acccgccgtc accgcttccg gacgctcagt
    13441 ggtatcgcgc atcaccagca ccggtttccc cagcgaaggc gcttcttcct gaatgccgcc
    13501 tgagtcggtc aaaatcagcc aggcgtggtt catcagccag acaaacggta aatactcctg
    13561 gggatcgatc agaatgacat ttttcacatg ccccagaatg cgattgaccg gctctctgac
    13621 gttcgggttg agatgcaccg gatagacaat ctggatgtcc tggtgcgtgg tggcaatgtc
    13681 tgccaacgcg tggcagattt cttcaaagcc acgaccgaaa ctttcacgcc tgtgaccggt
    13741 caccagaatc atctttttat cggggtcgat aaacgggtca tttgccgcca gttctgaacg
    13801 cagcgtatcg ctgctcatca cctggtcacg cacccataac agtgcatcaa taactgtatt
    13861 accagtaatg aagattcggc tatccgcaac gttttcacgc agcaagtttt gccgggaagt
    13921 ttcggttgga gagaagtgat acatcgccag atgcccggtc aatgtacggt tagcctcttc
    13981 cggccacggc gaataaagat cgcccgtgcg cagaccagcc tcaacgtgac caacaggaat
    14041 acgctgataa aacgccgcca ggctggttgc cagcgtcgtc gttgtgtcgc cgtgaaccag
    14101 cacgacgtct ggtttgaact cggcaagaat aggttttagc ccttccagaa tccgacaggt
    14161 tatctctgtc aggccctgtc ctggctgcat tatgttgaga tcgtagtcag gtacaatgga
    14221 aaagagtttc agcacctgat cgagcatctc ccgatgctgc gcagtgacgc aaactttagc
    14281 ctcaaaaaaa ggatcttttg ccaacgcatg caccaacggc gccatcttga tggcttccgg
    14341 gcgcgtacca aatacagtca gtactttcac atcgattctc ttcgaataag cggcgagcgc
    14401 ctttgcgctc accgcagcag tgttgctatt tcgagcaacg gcgggttaat gcgacaccag
    14461 ccccgatcag ccccccgaca atgccccaca taatcatcag gaaggcacga cgtgggctat
    14521 cgcgttttac cggttcttcc ggcgtacgca aatagcgata ggtctgaaaa cgcggatcca
    14581 gggttggacc aacattcagg gtgtttaaca tggcccgatt ctgatcatag tcgagatcaa
    14641 aggccggacc gacggcctgt aaattttcca gtcgagcctg gagcattgga cgcccaagca
    14701 ggaacatttc tgaatcaggt aattcctcgg caggcacatc tgtcgcactg cgcgaaatat
    14761 tatgctgctc agcaattttc agcgcctgtt caatactgtt catccggcgg tcgtagatgg
    14821 ctttcgccac ctcttcctga cgcttcacct gtgctttcat ctggatggta cgcgccgccc
    14881 atgcgccttt cagctcatca ttcagatggc tggctgcacg ctggctggca aaagcaacat
    14941 actgacgtaa caggttatta gcgtcaggcg cggtttcagc aataagcttc acgctgtcat
    15001 tgaccgcgcg ggtaaagtct ccggggataa actggatgtt gttaatcatt tcatccagca
    15061 acgccgcatc ggctttgctg ttgcccacca tccgctgttt gtaatagtcg gtttgcagcc
    15121 agaactctct gcgggtatcc cacgaggcca gttgcataac aaactcttta taggcttcgt
    15181 ccatgaccga tggttggtcg gcagaagcca tgtttgaacg gacatccagg ttacgcaaaa
    15241 attgctgctg cgagtaatat ccccccagca tattcaccgt tggacgatcg gtaatcgccg
    15301 tcgagctcca ctcctgacga gcaaaaaaag tatacgccag cgcgattaac gcaaatgcca
    15361 gccccatgcc aataatccat agcttcccag cccacaaggt acgaaacaac ccacgaatat
    15421 ccagttcatt ttcagcgtct tcggccggtt tcccaggcat tggttgtgtc atcacatcct
    15481 catttatttg gttaaattgg ggctgccacc acgatttcta cgcagtctgc gttttacgcg
    15541 cttaataaag cgagcaactt tccaggcacg cttaatgcaa tatccataga ggaagaatgc
    15601 tagcaaaaag agcaccagca tgacccactc cgggacaaaa tgagaatatt ctgccagcac
    15661 gccaatggaa gcgagcagtg ccgcagcaag ggtaatcagc acaaacgcct gacgggaagt
    15721 aaacccggca cgcatgatca aatggtgaat atgctgacgg tcaggagaga atgggctcat
    15781 gcctttacgc aggcgacggt acataatcgc caccatatcc attagcggaa tggcgattat
    15841 ccacaaagcg gtaaccgggc tgatgggatg ggttttgccc tgggtcgttt cgagcaggat
    15901 ccagataacg gtaaaaccaa tcagcgtact gcccgcatca cccataaaga ctttgtagcg
    15961 gcgacccagg ataccaaggt taagcatgat gtatggcagg atggcggcga tcatcgcaaa
    16021 gcaccagatt gcgaggctgg tttgcccgtc gaaccacaaa atcataccga ttgctgcaaa
    16081 cgagacgcag gacaacccgc ccagcaagcc atcaatgcca tcaaccatgt tgaacgcatt
    16141 aatggccgcc cagacggcaa atagcgtcag gaagtaacca aacggtccga gcaccatctc
    16201 ccaggagcca aagatataac ccaggctact gagataaagt ttgccgaaca ccatcataac
    16261 aatgccaaca gcggcctgta tggtggcacg gatttttacg ctgatatcaa aacggtcatc
    16321 cagcgcgcca atgaaaacaa gcacaccggc acaagcgaga tagagagatg catgcggaat
    16381 atagtaatcg acaattccga acgtgaagca aatccctgcg taaaccgaaa tccccccaac
    16441 gagaggtatc aatccctggt gacgtttgcg gaagtttggt ttatccacta aaccgacttt
    16501 ttttgccacc ttacgggcaa aaaacagaaa cagtgtcgtg aataaaaaaa tactgatgag
    16561 atcagtactc actgtcagta aattcacaat gcatgctctc agagaaaatt attagcagaa
    16621 gtataaccac gaagaccttt attcagaagg gaaagtccgt tccgaaccca ccagtcctgt
    16681 gcaaaaatta accatttgat atgcaaattg cataacacgc cagcaaattc caggaatttt
    16741 cctaacatcg ccagcgcggc attaagatta cagatataaa agcaaaacgc cacgtaaaca
    16801 cgtggcgttt ttggcataag acaaatttat gagcgtttca tcatttcgaa gaaatcgtca
    16861 ttggtcttgg tcatggccag tttattaatg aggaattcca ttgcatcgat ttcgcccatc
    16921 gggtgaatga ttttgcgcag gatccacatt ttctgcagtt cttcctgagt agtgagcagt
    16981 tcttctttac gggtaccgga acggttgtag tcgatagccg ggaagacgcg tttttcagcg
    17041 atcttacgag agaggtgcag ttccatgttg cctgtacctt taaactcttc gtagataact
    17101 tcgtccattt tagaaccggt atcgataagc gccgtcgcga taatagtcag gctgccgccc
    17161 tcttccacgt tacgcgccgc accaaagaag cgtttcggac gatgcagggc gttggcatcc
    17221 acaccaccgg tcaacacttt acctgacgcc ggaacaacgg tgttgtaagc gcgcgccaga
    17281 cgagtgatgg agtcgagcag aatgataacg tctttcttgt gctcaaccag gcgtttggcc
    17341 ttctcgatca ccatttccgc aacctgaacg tggcgagatg cgggttcgtc aaaggtagaa
    17401 gcaacaactt caccttttac cagacgctgc atctcggtta cttcttccgg acgttcgtcg
    17461 atcagcagaa ccatcagcac acaatccggg tggttgtaag caatgctctg ggcaatgttc
    17521 tgcagcagca tggttttacc ggctttcggc ggtgccacaa tcagaccacg ctgaccacga
    17581 ccgataggtg atgccagatc cagtacgcga gcagttaaat cttcagtaga accgttacca
    17641 cgttccatac gcagacgaga atttgcgtgc agcggggtta agttctcaaa gaggattttg
    17701 ttgcgggcgt tttcaggttt gtcgaagtta acttcgttaa ctttcagcag cgcaaaatag
    17761 cgttcacctt ctttcggcgg gcgaatctta ccagagatgg tatcaccagt gcggaggttg
    17821 aaacggcgga tttggctagg ggaaacgtag atgtcatcag gaccggcgag gtaggagctg
    17881 tctgcggaac ggaggaaacc aaatccatcc tgcaatatct ccagtacgcc atcaccaaag
    17941 atatcttcgc cactctttgc gtgctgcttc aggatggcaa aaataatgtc ctgcttacgc
    18001 atgcgagcca ggttttccag ccccatattt tcgccgagag tgatcagctc agaaaccggc
    18061 gtattcttta attcggtaag attcatagtg gtgtgagttc ttaaacttgg ggtaaatctc
    18121 gaacttaatg ttgtgaatgg tatggcagga tcatccatgc ctgtttacgg tcatcgactc
    18181 atgtcttttc gctgcctggt cacaggagag tacgcagaac tgaaacgaca agacggattg
    18241 agtgacgagc cagaaatctg ttcacttcgc atttaagata aaacgggaag cgttggaaat
    18301 tacaagattc aaacttaata aggtatgttt aatacgaagt caacactaag ttagcatgac
    18361 tcacgccggg cgtccagttt ttagcgacgg ggcacccgaa catgaaattc ccttacgcca
    18421 ggttagcgtc gaggaactct ttcaactgac ctttagacag tgcgcccact ttggttgccg
    18481 ccacttcacc gtttttgaac agcagcagag tcgggatacc acggatgcca tatttcggcg
    18541 cagtgccagg gttttgatcg atgttcagtt ttgcaacggt cagtttgccc tgatattcgt
    18601 cagcgatttc atccagaatc ggggcgatca ttttgcacgg accgcaccac tctgcccaga
    18661 aatcgacgag gatcgccccg tccgctttga gtacatccgt gtcaaaactg tcgtcagtca
    18721 ggtgaataat tttatcgctc atatataact ccacaggaat aagcctggcg tgttggtttc
    18781 gttgttggtg taacattaac caactaaagg ttgactttat ttcaccggat acgctttcgt
    18841 aaagcaatag taagctgata ttctaccaca ctatgagcaa aacacattta acagaacaga
    18901 agttttccga cttcgccctg catccgaagg ttgtagaagc ccttgaaaaa aaagggtttc
    18961 ataactgtac gcccattcag gcactggccc ttccgctgac gctggcgggt cgtgacgtag
    19021 ccgggcaggc gcaaaccggt accgggaaaa cgatggcgtt tcttacgtca acgtttcatt
    19081 atcttctctc tcatcctgcg attgccgatc gcaaggtgaa tcaaccgcgt gccttaatta
    19141 tggcaccgac gcgtgaactt gccgtgcaga tccatgccga cgcagaaccg ctggcggaag
    19201 ctactggcct gaagctgggt ctggcttacg gtggtgatgg ctacgacaaa cagctgaaag
    19261 tgctggaaag cggcgttgac attctgattg gcaccacggg tcgtttaatt gactacgcca
    19321 agcagaacca cattaacctc ggtgccattc aggtagtggt tctggatgaa gccgatcgca
    19381 tgtacgatct gggctttatt aaagatatcc gctggctgtt ccgccgtatg ccgcctgcaa
    19441 accagcgcct caacatgctg ttctccgcca cgctttcgta ccgggtacgt gaactggcgt
    19501 tcgagcagat gaacaatgcc gaatatattg aagtggaacc ggaacagaaa acgggccacc
    19561 gtattaaaga agagcttttc tacccttcta acgaagaaaa aatgcgtttg ctgcaaacgc
    19621 tgatcgaaga agagtggcca gacagagcga ttattttcgc caataccaaa caccgttgtg
    19681 aagagatctg gggccacctg gcggcagatg gtcatcgtgt cggtttgttg acaggcgatg
    19741 tcgcgcagaa aaaacgtctg cgtattcttg atgaatttac ccgtggcgat ctggatattc
    19801 tggttgccac cgacgttgcc gcgcgtggtt tgcatattcc ggcagtgacg cacgtcttta
    19861 actacgattt acccgatgac tgtgaagatt acgttcaccg tattggtcgt acaggtcgcg
    19921 caggcgcaag cggtcactct atcagcctgg cgtgtgaaga gtatgcattg aatttgcctg
    19981 ctattgaaac ctatattggt cactcaattc cggtaagcaa atacaatccg gacgcattga
    20041 tgaccgatct gccaaaaccg ctgcgcctca cgcgcccacg tacaggcaat ggtccgcgtc
    20101 gtactggcgc tccgcgtaat cgtcgtcgtt caggttaaat aaaatgtcat actcaaaatc
    20161 attcaaactg tatcaagatg gcaagagaat gaatcccgat gagcatactg tagtaagtga
    20221 ttcaggtgaa agaatgcagc caacacagag acagattgaa ggatgaagag tatgggttcc
    20281 acctcgtcgc tgtatgcagc cattgatctc ggttcgaata gttttcatat gctggttgtg
    20341 cgcgaggtgg ctggaagcat ccagacgctg acgcgaataa aacgcaaagt gcgtctggct
    20401 gctggcctga atagcgaaaa tgccctgtct aatgaagcaa tggagcgcgg ttggcaatgt
    20461 ctgcgcctgt ttgctgaacg tctgcaagat atccctccct cgcaaattcg cgttgtcgct
    20521 acggcgacat tacgtcttgc cgtcaatgcg ggtgatttta ttgccaatgc gcaggaaatc
    20581 ctcggttgtc cggtacaggt gatcagcggt gaagaggaag cacgtctgat ttatcagggc
    20641 gttgctcaca caactggcgg tgccgatcag cgcctggtgg tggatatagg cggtgccagt
    20701 actgaactgg taaccggcac gggtgcacaa accacctcgt tgttcagcct gtcgatgggc
    20761 tgcgtcacct ggctggaacg ctattttgcc gatcgtaatc tggggcagga aaattttgat
    20821 gctgcagaaa aagcggcacg cgaagtgtta cgtccggttg ccgatgaatt acggtatcac
    20881 ggctggaaag tgtgcgttgg tgcttccggc accgtgcagg cgttacagga aatcatgatg
    20941 gcacagggga tggatgaacg cattaccctg gaaaagttgc agcaattgaa acagcgagcc
    21001 attcattgcg gtcggctgga ggaactggag attgacgggc tgacgctgga acgtgcgtta
    21061 gtgttcccga gtggtctggc gatcctgatc gccattttta ccgaactgaa tattcagtgt
    21121 atgaccctgg cgggcggtgc gctgcgtgaa ggcctggtct acggcatgtt gcatctggcc
    21181 gtcgagcagg atattcgcag ccgtacgctg cgtaatattc agcgccgctt tatgatcgat
    21241 attgatcagg cgcagcgcgt agccaaagtt gcggctaact tcttcgatca ggtggaaaag
    21301 gaatggcatc ttgaagcaat aagccgcgat ttgctcatca gcgcctgtca gcttcatgaa
    21361 atcggcctga gcgttgactt taaacaagcg ccgcaacacg ctgcttatct ggtgcgtaat
    21421 ctggatcttc ccggttttac ccccgcacag aaaaaattgc tggcgacgct actgctcaac
    21481 cagactaatc cggtcgatct ctcatcgctg catcagcaaa atgccgtacc gccgcgcgtc
    21541 gcagaacaac tctgccgttt actccgcctg gccatcattt ttgccagccg tcgccgtgac
    21601 gatctcgtgc cagagatgac attacaggct aaccatgaac tgttgacctt gacgcttccg
    21661 caaggttggc taacccaaca tccgctgggt aaagagatta ttgctcagga aagccagtgg
    21721 cagagctatg tccactggcc gctggaagtg cattaatgag taagtgccgg atgcgatgct
    21781 gacgcatctt ttccggcctt gattatttcc ctcgttttgc cgccatcatc gctttcagat
    21841 tcgccagatg gctttgccct ttctgcatcc gttcttcggc gctgaccact ttgcgctcct
    21901 gttcccaaat cagatcatcc tgcggcagct ccagcaaaaa gcggctcggc tccgggcgca
    21961 ccagctcgcc gtactgacgg cgttctttac acagcgtaaa ggtcaattcc ttctgggcgc
    22021 gggtaatgcc gacataggcc agccgccgct cctcatcgat attatcttca tcgatgctgc
    22081 tctggtgcgg caaaaaccct tcttccatac cgaccatgta gacataagga aactccagcc
    22141 cttttgacgc gtgcagagtc atcagttgca cctgatccag ctcttcttca ctctcaccac
    22201 gctccatcat gtcgcgcaaa gtaaagcgcg tcaccacctg ggtgagcgtc atcggctcat
    22261 ccagttcgct gccttccagc atctccgtca tccagctaaa cagttggttg acgttcttca
    22321 tgcgcatttc ggcggctttc gggctgggcg atgtttcgta cagccaggat tcataatcca
    22381 tgccgtggat cagatcgcgc accgcggcaa tcggctcccg ctccgccaga cgctggattt
    22441 ctgccaacca gtgagtaaag cgggtcaatg cttcataacc acgtccgcta agcgtctgac
    22501 tcaggcccat atcaaagctg gcggtaaaca tgcttttatt gcgcgtcatc gcccactcac
    22561 ccagcttttt cagcgtcgcc gggccaatct ctctcttcgg cgtgttaacg atacgcagaa
    22621 atgcgctgtc atcgtccggg ttagtcagca cgcgcagata agccagcaag tccttgattt
    22681 caggacgaga gaaaaacgac gtaccaccag aaattttgta cgggatgcgg ttttgcatca
    22741 ggaatttttc aaacactcgc gactgatggt taccgcgata aagaatagcg taatctttgt
    22801 actgcgtttt attgacgaag tgatgcgcaa tcagttcacc agtgacgcgc tcggcttcat
    22861 gttcttcgtt attcgcgctt aaaactttaa gctctgtgcc gtaacccagt tcggagaaca
    22921 gacgcttctc aaagacgtgc gggttattgg cgatcaggat gttcgccgct ttcaaaatac
    22981 gtccggaaga gcggtagttc tgctcaagct taatcacctt cagcgccgga aaatcctgac
    23041 tcagcagcac caggttttgt ggacgtgcgc cgcgccagga gtagatcgac tggtcgtcat
    23101 cgcccaccac ggtaaagcgc gcgcggctgc ccaccagcag tttcaccagt tcatactggc
    23161 tggtgttggt gtcctgatac tcatccacca gcagatagcg aattttgttc tgccagcgct
    23221 cgcggacttc ttcattgcgt tgcagcagca acgtcggcaa taaaatcaga tcatcgaagt
    23281 cgagaacatt gcacgctttc aggtgtgcat catacagccc ataacaatgg gcaaaaatac
    23341 ggtcccgctc gccaatcgca ctggctgccg cctgggacgg tgttttgaga tcgttcttcc
    23401 agttagagat ggtcgaaatc aactgttgca ggagaacttt gtcatcttca atcagccctt
    23461 cggtcaactc tttgagcaac gccagctgat cggtatcgtc aaacagcgaa aagttcgcct
    23521 tcatccccag cgccgcatac tcacgcttga tgatatccag ccccagcgtg tggaaggttg
    23581 agatcatcag cccacgcgcc tctttgcgac ccagcgtctg tccgacacgc tctttcatct
    23641 cgcgcgccgc cttattggta aaggtcaccg ccgcgatatg tcgcgcctga taaccgcaac
    23701 cgcggatcag atgggcgatt ttattggtga tcacacgagt tttaccggaa cccgcgcccg
    23761 ccagcaccag gcaggggccg gtaacgaatt cgacagcttg ttgttggccg gggtttagac
    23821 gcataggtgt attgctcaat cttcgaacgg ggggaggatt gtagcagaat cgcatttgaa
    23881 tgtgaccgcg tgtaagccgc taaccgattg gctgcggatg tttaaatcag agagggaaga
    23941 ccgaaaccgg agttcatcgt gaatccatta gacttagaaa atatcgggtc gtaacgttgt
    24001 gttattactt tttcagagtt tactaaggag aggtgtactc gtggcgatta atttcagtcc
    24061 taaggttggt gaaatactgg attgcaactt tggtaactat cctgttagcc agaatggaca
    24121 gttcagcacc acgtattacg atggccgcat tcctcctgaa atgataaaaa acaggttagt
//
LOCUS       DAEEKQ010000051        23954 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.051, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000051 DAEEKQ010000000
VERSION     DAEEKQ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23954)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23954)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23954
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.051"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(39..401)
                     /locus_tag="KLK57_001937"
     CDS             complement(39..401)
                     /locus_tag="KLK57_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HBE0892308.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            938..2851
                     /gene="mtlA"
                     /locus_tag="KLK57_001938"
     CDS             938..2851
                     /gene="mtlA"
                     /locus_tag="KLK57_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HBE0892309.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            3081..4229
                     /gene="mtlD"
                     /locus_tag="KLK57_001939"
     CDS             3081..4229
                     /gene="mtlD"
                     /locus_tag="KLK57_001939"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBE0892310.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISDLDANSEVVSEAVTAYKAMQ"
     gene            4229..4816
                     /gene="mtlR"
                     /locus_tag="KLK57_001940"
     CDS             4229..4816
                     /gene="mtlR"
                     /locus_tag="KLK57_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HBE0892311.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(4828..5037)
                     /locus_tag="KLK57_001941"
     CDS             complement(4828..5037)
                     /locus_tag="KLK57_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892312.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            5322..5684
                     /locus_tag="KLK57_001942"
     CDS             5322..5684
                     /locus_tag="KLK57_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HBE0892313.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
     gene            6227..6905
                     /locus_tag="KLK57_001943"
                     /pseudo
     CDS             6227..6905
                     /locus_tag="KLK57_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
     gene            6949..11799
                     /locus_tag="KLK57_001944"
     CDS             6949..11799
                     /locus_tag="KLK57_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HBE0892314.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDSWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTASIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ETVTNLGEDALKWDKDNGVFTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTND
                     AVATNTTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDG
                     TVTATSSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALA
                     AINSSFSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLH
                     GVSSYVVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADA
                     GENGAFSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVN
                     ADGTITAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITN
                     VANGSISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIEENGAGINYVRTN
                     DNDLAFNDASASGVGATAVGYNAVASGASSVAIGQNSSSTVDTGIALGSSSVSSRVIA
                     KGSRDTSVTENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQ
                     NAIGAVATTPTKYFHANSTAEDSLAVGEDSLAMGAKTVVNGNAGIGIGLNTLVLADAI
                     NGIAIGSNARANHANSIAMGNGSQTTRGAQTGYTAYNMDAPQNSVGEFSVGSEDGQRQ
                     ITNVAAGSADTDAVNVGQLKVTDERVAQNTQSITNLNNQVTNLDTRVTNIENGIGDIV
                     TTGSTKYFKTNTDGVDANAQGKDSVAIGSGSIAAADNSVALGTGSVAEEENTISVGSS
                     TNQRRITNVAAGVNATDAVNVSQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGSTTR
                     ISNVSAGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAM
                     TGLPQAYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAAL
                     GAGIQW"
     gene            12167..13822
                     /gene="lldP"
                     /locus_tag="KLK57_001945"
     CDS             12167..13822
                     /gene="lldP"
                     /locus_tag="KLK57_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HBE0892315.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASKIDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            13822..14598
                     /gene="lldR"
                     /locus_tag="KLK57_001946"
     CDS             13822..14598
                     /gene="lldR"
                     /locus_tag="KLK57_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HBE0892316.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            14595..15785
                     /gene="lldD"
                     /locus_tag="KLK57_001947"
     CDS             14595..15785
                     /gene="lldD"
                     /locus_tag="KLK57_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HBE0892317.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            15981..16454
                     /gene="trmL"
                     /locus_tag="KLK57_001948"
     CDS             15981..16454
                     /gene="trmL"
                     /locus_tag="KLK57_001948"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HBE0892318.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(16507..17328)
                     /gene="cysE"
                     /locus_tag="KLK57_001949"
     CDS             complement(16507..17328)
                     /gene="cysE"
                     /locus_tag="KLK57_001949"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HBE0892319.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(17408..18427)
                     /gene="gpsA"
                     /locus_tag="KLK57_001950"
     CDS             complement(17408..18427)
                     /gene="gpsA"
                     /locus_tag="KLK57_001950"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HBE0892320.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(18427..18894)
                     /gene="secB"
                     /locus_tag="KLK57_001951"
     CDS             complement(18427..18894)
                     /gene="secB"
                     /locus_tag="KLK57_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HBE0892321.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSVVSRGTFPQLNLAPINFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(18957..19208)
                     /gene="grxC"
                     /locus_tag="KLK57_001952"
     CDS             complement(18957..19208)
                     /gene="grxC"
                     /locus_tag="KLK57_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HBE0892322.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(19350..19781)
                     /locus_tag="KLK57_001953"
     CDS             complement(19350..19781)
                     /locus_tag="KLK57_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HBE0892323.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            20026..21570
                     /gene="gpmM"
                     /locus_tag="KLK57_001954"
                     /gene_synonym="pgmI"
     CDS             20026..21570
                     /gene="gpmM"
                     /locus_tag="KLK57_001954"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HBE0892324.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            21580..22863
                     /gene="envC"
                     /locus_tag="KLK57_001955"
     CDS             21580..22863
                     /gene="envC"
                     /locus_tag="KLK57_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HBE0892325.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIATKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            22867..23826
                     /locus_tag="KLK57_001956"
     CDS             22867..23826
                     /locus_tag="KLK57_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HBE0892326.1"
                     /translation="MFPFRRNVLAFAALLALSSPVIAGKLAIVIDDFGYRPNNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNTVPDTPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKLNASVN"
BASE COUNT         6045 a         6109 c         6212 g         5588 t
ORIGIN      
        1 cagcaccacg taagttaaaa caatcaatag atccataatt agatctccgg gaacgtggaa
       61 tagtcagtct ttttctcggc ggcgagttga tgctctagcc gggaaattcg gtcagaaagt
      121 gcggcgattt ccgggtcagt cgctttctcc tgcgacgcaa catgtgactg catgccccac
      181 ccgcgctccg gttgatagag cgtcgcccag atccacagga acggccagat aacatgcagg
      241 gtaaacaggc tcacccagcc cgccgtatga atagcgtcgg catggggatg gttgcgcttt
      301 ttggcaatca gataagggat gtcatgtatc gcgatgattc cataaaaaat caccagaaag
      361 acgaagatca gcactcccaa cgcgaaatag tttagaaaca tatctgcctc ggattcacgt
      421 ttatcagtgt tgtttttggg ctggcagcca gaagggagtc aggctgatat tttgacaata
      481 atccgggttc gcgattctcg ccataacacc aaagaataat ttttagaggt gatgagttgc
      541 ttagttacat aacaattgta tgacgaaggc ataacatgct gtagatcaca tcaggtgaac
      601 gccgtaagaa aatatcttgt gattcagatc acaaagattc aacaaaccat caaaacaaaa
      661 atgtgacact actcacattt aaatgccatt tttagcgaaa atcgccgcct tgttgctttt
      721 ttacacaagc gttttgtgat gaacgtcacg ttaattacct ctctaccccc tatatttatg
      781 tgattgatat cacacaaaag gccgtcgact ggacagttaa ccgattcagt gccagatttc
      841 gcagtatcta caaggtccgg ctacctctgc cgccacatta acaaaaaacc tcgggcttcc
      901 agcctgcgcg acagcaaaca taagaagggg tgtttttatg tcatccgata ttaagatcaa
      961 agtgcaaagc tttggtcgtt tcctcagcaa catggtgatg ccaaatatcg gcgcgtttat
     1021 cgcgtggggt atcatcaccg cgttatttat tccaacaggg tggttaccga acgagacgct
     1081 ggcgaagctg gtcgggccga tgatcactta tctcctgccg ctgctgatcg gttataccgg
     1141 tggtaagctg gtaggcggcg aacgtggcgg cgtagtcggt gccatcacca ccatgggcgt
     1201 tatcgtcggc gcagacatgc cgatgttcct cggttctatg attgcaggtc cgctgggcgg
     1261 ctggtgcatt aagcacttcg accgctgggt agacggtaag atcaaatccg gttttgagat
     1321 gctggtgaat aacttctccg caggcatcat cgggatgatc ctcgctattc tggcattcct
     1381 cggcattggc ccgattgttg aagccctgtc caaaatgctg gctgcgggcg ttaacttcat
     1441 ggttgtccat gacatgctgc cgctggcgtc tatctttgtt gaaccggcga aaatcctgtt
     1501 cctcaacaac gccattaacc acggtatctt ctcgccgctg ggtattcagc agtcccatga
     1561 actgggtaaa tcaatcttct tcctgattga agctaaccca ggtccaggta tgggcgtgct
     1621 gctggcgtac atgttctttg gtcgtggtag cgctaaacag tctgcgggcg gtgcggcaat
     1681 catccacttc ctgggtggta tccacgaaat ctacttcccg tatgtgctga tgaatccgcg
     1741 tctgatcctc gcagtcatcc tcggcggtat gactggcgtg ttcacgctga ctatcctggg
     1801 cggtggtctg gtttctccgg catctccggg ttctatcctt gctgtactgg cgatgacacc
     1861 aaaaggtgct tatttcgcta acatcgcggg tgtgtgtgcg gcgatggctg tctccttcgt
     1921 tgtctctgct attttgctga aaaccagcaa agtgaaagaa gaagatgata ttgaagcagc
     1981 aactcgtcgt atgcaggaca tgaaagctga gtctaaaggc acatctccgc tgtctgcggg
     2041 cgatgtgact aacgacctga gccacgtacg taaaatcatc gttgcctgtg acgccggtat
     2101 gggttccagt gcgatgggcg caggcgtgct gcgtaagaaa attcaggatg caggtctgtc
     2161 acagatttcg gtcactaaca gcgcgatcaa caacctgccg ccagatgtgg acctcgtcat
     2221 cactcaccgt gacctgaccg aacgcgctat gcgccaggtt ccgcaggcgc agcatatttc
     2281 gctgaccaac ttcctcgaca gcggcctgta caccagccta accgaacgtc tggttgctgc
     2341 ccagcgccat actgaaaacg aagtgaaagt gaaagacagt ctgaaagaca gctttgacga
     2401 ttccagtgct aacctgttca aactgggcgc ggagaacatc ttcctcggtc gcaaagcagc
     2461 aaccaaagaa gaagcgattc gttttgctgg cgagcagctg gtgaaaggcg gttacgttga
     2521 gccggaatac gttcaggcga tgctggatcg tgaaaaactg accccgactt atctgggtga
     2581 gtctatcgcg gtgccacacg gtacggttga agcgaaagat cgcgtactga aaacgggcgt
     2641 ggtgttctgc cagtacccgg aaggcgtgcg cttcggtgaa gaagaagatg acattgcccg
     2701 tctggtgatt ggtattgcag cccgtaacaa cgagcacatt caggttatca ccagcctgac
     2761 caatgcactg gatgatgagt ccgtcatcga gcgtctggca cacaccacca gcgtggatga
     2821 agtgctggaa ctgttggcag gtcgtaagta atccaatccc accctctcca catggagaag
     2881 gtggggttaa ttgcctgatg cgctacgctt atcaggccta caggatgcat cacgatttgt
     2941 tgaatttgca cgttcttgta ggccggataa ggcgcttacg ccgcatccgg cgctgcccct
     3001 ctcctcacgg agagggtttg ggtgagggaa aagcctcacc ccagccctct cgggtaaaaa
     3061 cattgatgaa ggttaatact atgaaagcat tacattttgg cgcaggtaat atcggtcgtg
     3121 gctttatcgg taaactgctg gcagacgcgg gtatccaact gacgtttgcc gatgtcaatc
     3181 aggtggtact tgatgccctg aatgcccgtc atagctatca ggttcatgtg gttggtgaaa
     3241 ccgagcaggt agataccgtt tccggcgtca atgctgtcag cagcattggt gatgatgtcg
     3301 ttgatctgat tgctcaggtc gatttagtca ctaccgccgt tggcccggtt gtgctggaac
     3361 gtattgctcc ggctatcgcc aaagggctgg tgaaacgcaa agaacaaggt aatgaatccc
     3421 cgctgaacat catcgcctgt gaaaacatgg tacgcggtac cacgcagctg aaaggccatg
     3481 tgatgaacgc cctgccggaa gacgccaaag cgtgggtaga agaacacgtt ggctttgtcg
     3541 attccgccgt tgaccgcatc gtaccgcctt cggcttcggc aactaacgat ccgctggaag
     3601 tgacggtaga aactttcagc gaatggattg tcgataaaac gcagttcaaa ggcgcactgc
     3661 cgaacatccc aggcatggag ttaaccgaca acctgatggc atttgtcgaa cgtaaactct
     3721 tcaccctgaa cacgggtcat gctataaccg cgtacctcgg aaaactggcc ggtcatcaga
     3781 ccattcgtga cgctattctc gacgagaaaa tccgcgcggt ggtaaaaggt gcgatggaag
     3841 aaagtggtgc ggtactgatc aagcgctacg gctttgacgc agacaaacat gcggcgtaca
     3901 tccagaaaat cctcggtcgt tttgagaacc cgtatctgaa agatgatgta gagcgcgtag
     3961 gccgtcagcc gctgcgtaaa ctgagtgctg gcgaccgtct gatcaagcca ttgctcggta
     4021 cgctggaata cagccttccg cacaagaatc tgattcaggg gattgctggt gcaatgcact
     4081 tccgcagtga agatgatccg caggctcagg aactggcagc actgatcgct gacaaaggtc
     4141 cgcaggcggc gctggcacag atttccgatc ttgatgccaa cagcgaggtt gtatccgagg
     4201 cggtaaccgc ttataaagca atgcaataat ggtggaccag gcgcaggaca ccctgcgccc
     4261 gaataacaga ttgtcagata tgcaggcaac aatggaacaa acccaggcct ttgaaaaccg
     4321 tgtgcttgag cgtctgaatg ctggcaaaac cgtgcgaagc tttctgatca ccgccgtcga
     4381 gcttttgacc gaggcggtaa atcttctggt gcttcaggta ttccgcaaag acgattacgc
     4441 ggtgaagtat gctgtagaac cgttactcga cggcgacggt ccgctgggcg atctttctgt
     4501 gcgtttaaaa ctcatttacg ggttgggcgt cattaaccgc caggaatacg aagatgcgga
     4561 gctgctgatg gcattgcgtg aagaactaaa tcacgacggc aacgagtacg cctttaccga
     4621 cgacgagatc cttggaccct ttggtgaact gcactgcgtg gcggcgttac caccgccgcc
     4681 acagtttgaa ccagcagact ccagtttgta tgcaatgcaa attcagcgct atcaacaggc
     4741 tgtgcgatca acaatggtcc tttcactgac tgagctgatt tccagaatca gcttaaaaaa
     4801 agcctttcaa aagtaagcaa cgtctgctta ctgcccctct acctgcttcg gccgataaag
     4861 ccgacgataa tactccagac gttgtagata taacggcacg ctttcatcgg gtattccgga
     4921 cggaatcgcg ttacggggag gaagtttttt cagatactcc cggaacgcct ggcttgatgc
     4981 catgaaatct acggctttat cgataagaag cggaacgttt tcacctaatt tgcccatgat
     5041 gatatctccg tattaccccg cgccgggaat gcgcggccgc caattttagt ttaggttccc
     5101 ggtaaagatg acattaagga aaacgtgctg aatcctcaat aatcatccgc tgctgtttat
     5161 tatctttttt ctatagattt cttaattaat caataaattg cgtagttttt aagcatgtta
     5221 atgattacgt tacagaacta ttgcaatgtc atcagttgtg aacaaagcac ctggtcgcgc
     5281 atactagggg ctataaattt atctttatca gaagccatca catgaaagaa gtcgaaaaaa
     5341 acgaaatcaa acgtctcagc gatcgtctgg acgccatccg ccaccagcag gccgatctgt
     5401 cgctggttga agccgcagac aaatatgccg agctggaaaa agagaaagcc acgctggaag
     5461 cagaaattgc tcgcctgcgt gaagttcata gccaaaaact gagtaaagaa gcacagaagc
     5521 tgatgaagat gccgtttcag cgcgcaatta ccaaaaaaga gcaggctgat atgggcaagc
     5581 tgaagaaaag tgttcgcgga ctggtggttg tgcacccaat gaccgcgctg ggccgtgaaa
     5641 tgggcctgca ggagatgact ggattctcta agactgcgtt ttaagcgcct cagacgactg
     5701 gttcgatcat atcaacccgc atctggcttg ccttagctgg tcaatgcggg gactctcctg
     5761 tctactcgcc taattaccaa acaattcact ccctttacgt cgatttaccg ctggttgata
     5821 ttctgaaaat gcatttttac cgaaatagcc acctataaac aataaaaata tatagaagat
     5881 atcatttatt aattaacaag cagttacgtc tctttttttc ggatttaatt gtgaaatttt
     5941 aatcatcata ttcatacaat aaattatctg taaatttttt cccagacatg gtttctttca
     6001 tatttgacat ttattaataa tcatcattag aatccacgaa aataatatat tcatacaaac
     6061 acagacatct tttgaaacga atgatacatt tttatgatga atgcttaaaa caatatttaa
     6121 tcgacacaca tcactgtcat taatgtaaag caacatagat caccaataat acaatggaca
     6181 actctaccgg cattgatcgc tggtcggagg aaataaggat ttttacatga acttgaagaa
     6241 gatttttttc agtgctgtca ctgtttcagt tctctgcgca ttaacgggat gtgactacat
     6301 tgaagaaggc aagccagaaa gtagtttatt gaaacaacaa gaagaacatt aaaattgatc
     6361 tacttgaaaa acaacaagca cagctaaaga gtcaactgga aactattcaa aaacaacaaa
     6421 ctggcataat aagcagtaca aagactttaa ctcacgtaat taaatccgtt aaagatcaac
     6481 aaaacacttt tatttttacc gagtttaacc cagcaaaaac gaagtatttc atcctgaaca
     6541 atggttcggt ggctttagcg ggtcgagtgt tatccattga cgccacagag aatggcagtg
     6601 ttattcatat ttcactggtc aacttattaa gtacacctat ctcaaatatt ggttttaatg
     6661 cgacatgggg tggtgaaaaa cctgtcgatg ccaaagagtt tgcccggtgg caacaattac
     6721 ttttcaacac atcaatgaaa tccacattga aattattacc aggtcaatgg caagacatta
     6781 atttgaccct gaagggtgta tcgcccaata acctgggata tctgaagtta gccatcaaca
     6841 tggaaaatat tcagttcgac aatcttccct ctgctgaaaa tcggcagaaa agaagcaaaa
     6901 aataaatgca gttagtacgc ttataacaag gaatagcgag catcattaat gaacaaaata
     6961 tttaaagtta tctggaaccc tgcgacaggg aattatactg ttaccagcga aacggcaaaa
     7021 agccgtggca agaaatctgg gcgcagtaag ctgttaattt ctgcgctggt tgcgggtgga
     7081 atgttgtcgt cgtttggggc attggcgaat gccgggaatg acaacggcca gggtgttgat
     7141 tacggtagtg gatcagctgg cgacagctgg gttgctatag gcaaaggggc gaaagcaaat
     7201 acttttatga acaccagtgg ttccagtact gctgtgggtt atgacgctat agctgaaggc
     7261 caatatagct ctgccatcgg gtcaaaaacc catgcgattg gtggtgcatc aatggccttt
     7321 ggggttagtg caatatcaga aggcgataga agtatagcac tgggtgcctc ttcgtattca
     7381 ttgggccaat actcaatggc cctcggccgt tattcaaaag cattgggtaa attgtctatt
     7441 gctatggggg actcttccaa agcggaagga gcaaacgcca ttgccctggg aaatgccact
     7501 aaagctactg agattatgag tattgctctt ggcgacaccg ccaatgcgtc aaaagcgtat
     7561 tcaatggcgc tgggagcaag tagcgtcgca tctgaagaaa acgcaattgc cctggggcgt
     7621 agcagtgtag ctagcggtac tgacagcctc gcatttggca gacaatcact tgccagcgca
     7681 gcgaacgcta ttgcgatagg tgctgagacc gaagccgctg aaaatgcaac tgctattggc
     7741 aataatgcga aggcaaaagg gactaatagc atggcaatgg ggttcggaag ccttgccgat
     7801 aaagtcaata ctatcgcatt aggaaatggc agccaggctc tggcagataa tgcaatcgcc
     7861 ataggccagg gcaacaaagc tgatggcgtg gatgccatcg ctctgggtaa tggtagccag
     7921 tcgagaggct taaacaccat tgccttaggc acagccagta atgcaactgg tgataagagt
     7981 cttgcgcttg gtagtaatag cagtgccaac ggtattaact ctgtcgcgct gggcgcagat
     8041 tccattgcgg atttagacaa taccgtctct gtcggcaata gttcattaaa acgcaagatc
     8101 gttaatgtga aaaatggcgc gatcaagtct gacagttacg atgccattaa tggttcacag
     8161 ctttatgcca ttagcgactc ggtagcaaaa aggcttggag gaggggctgc agtagatgtt
     8221 gatgacggta ctgttacagc accaacctac aatttaaaaa atggtagcaa aaataacgta
     8281 ggggctgcgc tcgctgtact tgatgaaaac accctgcaat gggaccaaac caaaggcaaa
     8341 tacagcgctg ctcatggtac tagtagccca actgccagcg taatcaccga tgttgcggat
     8401 ggcacgattt cagcctccag taaggatgcg gttaacggtt cccaactgaa agctaccaat
     8461 gacgatgtcg aagccaacac cgccaatatc gctactaata ccagcaacat tgccacgaat
     8521 acagcaagta ttgccaccaa taccaccaat atcaccaacc tgacggattc cgttggtgac
     8581 cttcaggctg atgccctgct ctggaacgaa actaaaaagg cattcagtgc agctcacggc
     8641 caggatacca ccagcaaaat caccaacgtt aaagatgccg acctgacggc tgacagcact
     8701 gatgctgtta acggctctca gctgaaaacc accaacgatg ctgtggcgac gaataccacc
     8761 aatatcgcca ataacacttc caatattgcc actaacacca ccaacatctc taatctgact
     8821 gagacggtga ctaatcttgg tgaggatgcg ctgaaatggg ataaggacaa tggtgtattc
     8881 acggcagctc atggcaccga gaccaccagc aaaatcacca acgttaaaga tggcgacctg
     8941 acgactggca gcaccgatgc cgttaacggc tctcagctga aaaccaccaa cgatgccgtg
     9001 gcgacgaata ccaccaatat cgccactaac accaccaaca tctctaatct gactgagacg
     9061 gtgactaatc ttggtgagga tgcgctgaaa tgggataagg acaatggtgt cttcactgca
     9121 gctcatggca acaataccgc cagcaaaatc accaatatcc tggacggcac agtcactgca
     9181 accagttccg atgccattaa cggtagccag ctttatgact taagcagcaa tatcgccacc
     9241 tacttcggcg gcaatgcttc tgtgaatact gacggtgtgt ttaccggtcc aacctacaaa
     9301 atcggtgaaa caaattatta taacgtcggc gatgcactgg ctgcgattaa ctcctcattt
     9361 agcacgtctc tcggcgatgc tctgctttgg gatgccaccg caggtaaatt cagtgccaaa
     9421 cacggtacta atggtgacgc aagcgtgatc actgatgtcg cagatggtga aatttcagac
     9481 tccagttctg acgcagtaaa cggctcacaa ctccacggcg tgagcagtta tgttgttgat
     9541 gcgctggggg gtggtgccga agtcaatgca gacggcacca tcactgcgcc gacgtacacc
     9601 attgctaatg ctgattacga taatgtcggt gatgccctga atgctatcga taccactctt
     9661 gacgacgctc tgctctggga tgcggacgcc ggtgaaaatg gtgcatttag cgccgctcac
     9721 ggaaaagata aaactgccag tgtaatcact aacgtcgcta acggtgcaat ctctgctgcc
     9781 agcagcgacg cgattaacgg ctcacaactc tataccacca ataagtacat cgctgatgcg
     9841 ctgggtggtg acgcagaagt caacgctgac ggcaccatca ccgcaccgac ttacaccatt
     9901 gcgaacgccg agtacaacaa cgtcggtgac gccctggatg cgcttgatga taacgccctg
     9961 ctgtgggatg agactgccaa tggcggtgct ggagcctaca atgccagcca tgacggtaaa
    10021 gccagcatca tcactaatgt cgctaatggc agtattagtg aggacagtac cgatgcagtg
    10081 aacggttctc agttgaatgc gacgaatatg atgattgagc agaacaccca aattatcaat
    10141 cagctcgctg gtaacaccga cgcaacctat atcgaagaaa atggcgctgg tatcaactac
    10201 gttcgtacta acgacaacga tttagccttt aacgatgcaa gcgcctctgg tgtcggcgct
    10261 acagctgtag gttataacgc tgtcgcgtct ggtgccagca gcgtagccat tggtcagaac
    10321 agcagcagca ccgttgatac cggtattgcg ctgggtagca gctccgtttc cagccgtgtg
    10381 attgccaaag gttctcgtga cactagcgta acggaaaatg gcgtggtgat tggttatgac
    10441 accactgacg gcgaactgct aggtgcattg tcaattggtg atgacggtaa ataccgccaa
    10501 atcatcaacg tagctgatgg ttcagaagcc catgacgccg ttacggttcg ccagttgcag
    10561 aacgctattg gagcggtcgc cactacgcca accaagtact ttcacgccaa ctcaacggca
    10621 gaagactcac tggccgttgg tgaagactcg ctggcaatgg gtgcgaaaac tgtcgttaat
    10681 ggtaatgcag gtattggtat cggtttgaac actctggttc tagctgatgc gatcaacggc
    10741 attgctatcg gcagtaacgc acgcgcaaac catgcaaaca gtatcgcaat gggtaacggt
    10801 tctcagacca cccgtggtgc acagactggc tacaccgcct acaacatgga cgcaccgcag
    10861 aactctgtag gtgagttttc tgtcggtagt gaagacggtc aacgtcagat cacaaacgtc
    10921 gcagctggtt cagcggatac cgatgcggtt aacgtgggtc agttgaaagt cactgatgag
    10981 cgcgtagcgc aaaataccca gagcattact aacctgaaca atcaggtcac taatctggat
    11041 actcgcgtta ctaatatcga aaacggtatt ggcgacattg tcaccaccgg tagcaccaag
    11101 tacttcaaga ccaacaccga tggcgtagat gccaacgccc agggtaaaga tagcgttgct
    11161 attggttctg gttccattgc tgccgctgac aacagcgtcg cactgggtac cggttccgtt
    11221 gcagaggaag aaaatacaat ctctgtaggt tcttctacta accaacgccg tatcaccaac
    11281 gtagctgccg gtgttaatgc caccgatgcg gttaacgtgt cgcagctgaa atcttctgaa
    11341 gcaggcggcg tgcgctacga caccaaagct gatggttcta tcgactatag caatatcacc
    11401 ctcggtggtg gcaacggcag tacgactcgt atcagcaacg tctccgctgg cgtcaacaac
    11461 aacgacgcgg tgaactacgc gcagttgaag caaagcgtgc aggaaacgaa gcaatacacc
    11521 gatcagcgga tggttgagat ggataacaaa ctgtctaaaa ctgaaagcaa gttgagcggt
    11581 ggtatcgctt ctgcaatggc aatgaccggt ctgccgcagg cttatacacc gggtgccagc
    11641 atggcttcta ttggtggcgg tacttacaac ggtgaatcgg cagttgcttt aggtgtatcg
    11701 atggtgagcg ccaatggtcg ttgggtctac aaattacaag gtagtaccaa tagccagggt
    11761 gaatactccg ccgcactcgg tgccggtatt cagtggtaat gatccattaa gttagtgtga
    11821 ctaagggcaa acgccaaatt gcctgatgcg ttacgcgcat caggcaagat aaggcgcccc
    11881 gggtcaacaa tctaagggca attctctgat gaggattgcc cttttcttta ccagacatct
    11941 ccccccacaa gaattggccc taccaattct tcgcttatct gacctctggt tcacaattcc
    12001 ccaattaaaa ctcacatcaa tattgccaat acataacatt tagttaacca ttcattgtca
    12061 ttatccctac acaacacaat tggcagtgcc acttttacac aacgtgtgac aaggagatga
    12121 gcaacagact cattacacga tgtgcgtgga ctccaggaga cctgcaatga atctctggca
    12181 acaaaactac gatcccgccg ggaatatctg gctttccagt ctgatagcat cgcttcccat
    12241 cctgtttttc ttctttgcgc tgattaagct caaactgaaa ggatacgtcg ccgcctcatg
    12301 gacggtagca atcgcccttg ccgtggcttt gctgttctat aaaatgccgg tcgctaacgc
    12361 gctggcctcg gtggtttatg gcttcttcta tggtttgtgg cccatcgcgt ggatcattat
    12421 tgcagcagtg ttcgtctata agatctcggt gaaaaccggg cagtttgaca tcattcgctc
    12481 gtctattctt tcgataaccc ctgaccagcg tctgcaaatg ctgatcgtcg gtttctgttt
    12541 cggcgctttc cttgaaggag ccgcaggctt tggcgcaccg gtagcaatta ccgccgcatt
    12601 gctggtcggc ctgggtttta aaccgctgta cgccgccgga ctgtgcctga ttgttaacac
    12661 cgcgccagtg gcatttggcg cgatgggcat tccaatcctg gttgccggac aagtaacagg
    12721 catcgacagc tttgagattg gccagatggt ggggcggcag ctaccgttta tgaccattat
    12781 cgtgctgttc tggatcatgg cgattatgga cggctggcgc ggtatcaagg agacgtggcc
    12841 tgcggtcgtg gttgcgggcg gctcgtttgc catcgctcag taccttagct ctaacttcat
    12901 tgggccggag ctgccggaca ttatctcttc gctggtatca ctgctctgcc tgacgctgtt
    12961 cctcaaacgc tggcagccag tgcgcgtatt ccgttttggt gatttggggg cgtcaaagat
    13021 tgatatgacg ctggcccaca ccggttacac tgcgggtcag gtgttacgtg cctggacacc
    13081 gttcctgttc ctgacagcta ccgtaacact gtggagcatc ccgccgttta aagccctgtt
    13141 cgcatcgggt ggtgcgctgt atgagtgggt gatcaacatt ccggtgccgt acctcgataa
    13201 actggttgcc cgtatgccac cagtggtcag cgaggctaca gcctatgccg ccgtgtttaa
    13261 gtttgactgg ttctctgcca ccggcaccgc cattctgttt gctgcactgc tctcgattgt
    13321 ctggctgaag atgaaaccgt ctgacgctat cagcaccttc ggcagcacgc tgaaagaact
    13381 ggctctaccc atctactcca tcggcatggt gctggcgttc gcctttatct cgaactattc
    13441 cggactgtca tcaacactgg cactggcact ggcgcacacc ggtcatgcat tcaccttctt
    13501 ctcgccgttc ctcggctggc tgggggtatt cctgaccggg tcggatacct catctaacgc
    13561 cctgttcgcc gcgctgcaag ccaccgcagc acaacaaatt ggcgtctctg atctgttgct
    13621 ggttgccgcc aataccaccg gtggcgtcac cggtaagatg atctccccgc aatctatcgc
    13681 tatcgcctgt gcggcggtag gcctggtggg caaagagtct gatttgttcc gctttactgt
    13741 caaacacagc ctgatcttca cctgtatggt gggcgtgatc accacgcttc aggcttatgt
    13801 cttaacgtgg atgattcctt aatgattgtt ttacccagac gcctgtcaga cgaggttgcc
    13861 gatcgtgtgc gggcgctgat tgatgaaaaa aacctggaag cgggcatgaa gttgcccgct
    13921 gagcgccaac tggcgatgca actcggcgta tcacgtaatt cactgcgcga ggcgctggca
    13981 aaactggtga gcgaaggcgt gctgctcagt cgacgcggcg gcgggacgtt tattcgctgg
    14041 cgtcatgaca catggtcgga gcaaaacatc gtccagccgc taaaaacgct gatggccgat
    14101 gatccggatt acagtttcga tattctggaa gcccgctacg ccattgaagc cagcaccgca
    14161 tggcatgcgg caatgcgcgc cacacctggc gacaaagaaa agattcagct ttgctttgaa
    14221 gcaacgctaa gtgaagaccc ggatatcgcc tcacaagcgg acgttcgttt tcatctggcg
    14281 attgccgaag cctcacataa catcgtgctg ctgcaaacca tgcgcggttt cttcgatgtc
    14341 ctgcaatcct cagtgaagca tagccgtcag cggatgtatc tggtgccacc ggttttttca
    14401 caactgaccg aacaacatca ggctgtcatt gacgccattt ttgctggtga tgccgacggg
    14461 gcgcgtaaag caatgatggc gcaccttagt tttgttcaca ccaccatgaa acgattcgat
    14521 gaagatcagg ctcgccacgc acggattacc cgcctgcccg gtgagcataa tgagcattcg
    14581 agggagaaaa acgcatgatt atttccgcag ccagcgatta tcgcgccgca gcgcaacgca
    14641 ttctgccgcc gttcctgttc cactatatgg atggtggtgc atattctgaa tacacgctgc
    14701 gccgcaacgt ggaagatttg tcagaagtgg cactgcgcca gcgtattctg aaaaacatgt
    14761 ccgacttaag cctggaaacg acgctgttta atgagaaatt gtcgatgccg gtagcactgg
    14821 caccggtggg tttgtgtggc atgtatgcgc gtcgtggcga agttcaggca gccaaagcgg
    14881 cggacgcgca tggcattccg tttactctct cgacggtttc cgtttgcccg attgaagaag
    14941 tcgcgccagc catcaagcgc ccaatgtggt tccagcttta tgtactgcgc gatcgcggct
    15001 ttatgcgtaa cgcgctggag cgagcaaaag cagcgggttg ttcgacgctg gttttcaccg
    15061 tggatatgcc gacaccgggc gcacgctacc gtgatgcgca ttcaggtatg agcggcccaa
    15121 atgcggcaat gcgccgctac ttgcaggcgg tgacgcatcc gcaatgggcg tgggatgtgg
    15181 gcctgaacgg tcgtccacat gatttaggta atatctcagc ttatctcggc aaaccgaccg
    15241 gactggaaga ttacatcggc tggctgggga ataacttcga tccgtccatc tcatggaaag
    15301 accttgagtg gatccgcgat ttctgggatg gcccgatggt gatcaaaggg atcctcgatc
    15361 cggaagatgc gcgcgatgca gtacgttttg gtgctgatgg aattgtggtt tctaaccacg
    15421 gtggccgcca gctggacggt gtactctctt ccgctcgtgc gttgcccgct attgcagatg
    15481 cggtgaaagg tgatatcgcc attctggcgg atagcggaat tcgtaacggg cttgatgtcg
    15541 tgcgtatgat tgcgctcggt gccgacaccg tactgctggg tcgtgctttc ctgtatgcgc
    15601 tggcaacagc gggccaggcg ggtgtagcta acctgctaaa tctgatcgaa aaagagatga
    15661 aagtggcgat gacgctgact ggcgcgaaat cgatcagtga aattacgcaa gattcgctgg
    15721 tgcaggggct gggtaaagag ttgcctacgg cactggctcc gatggcgaaa gggaatgcgg
    15781 catagtcgtt tgcgccccct caccctaacc ctctccctca gggagagggg accgttcggc
    15841 gctgtatgta ctccctcact ctgaaacgac accgcactct ttttttctcc ctcgcccctc
    15901 cggggagggc cggggtgagg ggaaaaggcc gcacggttca ccaattttct gctatcctgc
    15961 ccccgcacta agggggcagt atgctaaaca tcgtacttta cgaaccagaa attccgccaa
    16021 atactggcaa catcatccgt ctttgcgcta ataccggctt tcgtctgcat atcatcgaac
    16081 cgatgggatt tgcctgggac gataagcgcc tgcgccgcgc ggggctggac tatcacgagt
    16141 ttaccgccgt tacgcgtcat catgactatc gcgcgttcct cgaagcagaa aatccccagc
    16201 gcctgtttgc cctcaccacg aaaggtacgc ctgctcacag cgccgtaagc tatcaggatg
    16261 gcgattatct gatgttcggc ccggaaacac gcggcctgcc agcgagcatt cttgatgccc
    16321 tgcccgctga acaaaaaatt cgcattccga tggtgccgga cagccgcagc atgaatctgt
    16381 ccaatgcggt gtcggtagtg gtgtatgaag cctggcggca gctgggatat ccgggagcgt
    16441 tgttgagaga ttagttactg tatgccggat agatgattac atcgcatccg gcacgatcac
    16501 aggacattag atcccatccc catactcaaa tgtatggtta ataccgttga aatgctggtc
    16561 catatccatt gatggcttat cgctgtctgg tttaccgaca atacgagccg gaacgccagc
    16621 ggcggttgta tgcggcggca ccggttgcag caccacggaa cctgcgccaa tcttcgcgcc
    16681 gcgaccaact tcaatattgc cgaggatttt cgcgcccgcg ccaatcatca caccttcacg
    16741 aattttcggg tgacggtcac caccagattt acccgtaccg ccaagcgtca cagattgcag
    16801 aatcgatacg tcgttttcaa ttaccgccgt ttcacccaca acgatgcccg tggcgtggtc
    16861 gagcataatg ccacggccaa tttttgctgc cgggtgaata tcgacctgga acgtcacaga
    16921 aacctggttt tgcagaaaga ttgccaatgc gcgacgcccc tgattccaca accagtgacc
    16981 gatgcgatag gcctgcaagg catgaaaacc cttcaggtat aacaacgggg ttgagtattt
    17041 atcgactgcc gggtcgcggg tacgcaccgc ctgaatatca caggccgcag aggcgatcat
    17101 ttccgggtca gcggcgtagg cttcttccac cacttcacgg atagcaatag caggcataat
    17161 tggcgatgac agcttgttcg ccagcatgta gctcagtgca ctaccaaggt tttcgtgctt
    17221 gagtagcgtc gcgtggtaaa aactggccag cattggctca cagtccgcca gcgttctggc
    17281 ttcggcttta atattgttcc agacaatttc cagttcttca cacgacattg cttactccac
    17341 acgatgagat aatgaccggc ccgttctgcg cgggccgggt catagcggta acaaaggttc
    17401 cctggggtta gtggctgctg cgctcgtcct tgcgtgcacg acctaataac gtcaatgctg
    17461 cctcgcgcgc gttttttccg caatataata cttgataaat ttcctcggtt attggcattt
    17521 ctacgccgaa gcgatgcgcc agttcgcgga cttctttcgt attgcggtag ccttccacca
    17581 cctgaccaat cttctcctgc gcgctttgta catccatgcc ctgaccgagc atcatgccaa
    17641 aacggcggtt acgcgactgg ttgtcggtac aggtaagcac cagatcgccc agccccgcca
    17701 tgcccataaa ggtggcagga tcggcaccca gcgccgcgcc aagacgcgac atttcagcca
    17761 gcccacgggt gatcagtgcc gtacgcgcat tcgcaccaaa accgataccg tcggacatcc
    17821 ccgcgccaat ggcaatgacg tttttcaccg caccgccaag ctgcacaccg atgaaatccg
    17881 ggttgctgta aacgcggaaa cttttgccac agtgcagcaa ttgttggaga tcatcggcaa
    17941 aggtctggtc ggtagaggcc aacgaaatag ccgtcggtaa acctgccgcc agttctttcg
    18001 caaacgttgg gccagagata accgccagcg gaatttgatc gcctaaggcc tcacgcgcca
    18061 cgtcctgtaa cagacgtccg gtttccgctt ccagcccttt ggtcgcccac accagacgcg
    18121 catcaggacg catcagcggt ttaatctggc gcagcacttc accaaagaca tggctgggta
    18181 cgacgacgag aatattacgg ctggctgcca gcgcagtggc gagatcgctt tcaagatgga
    18241 gcgtatcggg aaaaggcaca tcggggagaa acgcggcgtt acagcggtcg cgttcaagcg
    18301 ttgcgatatg ttcagggtca tggccccaga ggacaacctc gtggccattt cttgccaggg
    18361 taatggcaag agcggtgccg tacgagccgg caccgatcac agtcattgaa gcattacgtt
    18421 ggttcatcag gcatcctgat gttcttcagt accttcgcca gcctgctgct gcaaatagtt
    18481 catgaacagc gcatcgaagt taatcggcgc aaggttcagt tgcgggaatg taccgcggga
    18541 taccacgctg gtgatgcact cacgagcata cgggaacaga atgttcgggc agtatgcgcc
    18601 caggcaatgt gccatctggg tgccttcgat acccgcgatg gagaaaatac cgccctgctg
    18661 aacttcacac aggaacgcgg tttcttcgcc cagggaggcc gttacggtaa cacgcagtac
    18721 cacttcgtat acgtcatctg ccagttggga agatgccgta tccagatcaa gtttaacttc
    18781 tggttgccaa tctttctgga aaacgtgcgg cgcgttcggc gcttcgaaag agatatcctt
    18841 ggtataaata cgttggatct ggaaagtcat ttcagtgttg ttttgttctg acatgtgtag
    18901 aaaaccctta agtgttgtcc ttaaatactg cgcaatgtaa tgccaacgtt cacacgttat
    18961 ttcagcaggg gatccagtcc accacgtgca tccaatgcat acaagtcatc acagccgcca
    19021 atgtgctgtg cgtcaataaa aatctgggga accgtggtgc gaccgctgcg tttgatcatc
    19081 tcttcacgct tggcggcatt gccatcgatc ggcaactcct gaaaactcac gcccttgctg
    19141 ctcagcagtg cttttgcacg atggcaatac gggcaggttt ctttggtata gatttcaaca
    19201 ttggccataa tttagctcct gtttttttac cctgaaattt catgttgcag ggcggctgca
    19261 aaatggagtg tccccagaag cttataacaa taagtcgctg ggggcctaat tgcagctaac
    19321 ggcctgcatc atgaaagacg acaggtaaat tatttgccgc gcaccagagg caagttttcg
    19381 ccagcccagc cagcaacgcc ctctttcagc acgaatactt gcgcaaaacc agctttcgtc
    19441 agcgcgtttg caggctcctg gcactgcatg ccagaaccgt ctaccacgat aaccggtttg
    19501 tctttgtgct tctcaagctc accaacattg ttggctttga tttcgctcgg caacagattg
    19561 atagaacctg cgatatggcc tttacggaag tcatcacgct gacgtaaatc cacaaccaca
    19621 gcgtcttctt tgttgatcag acgcgtagct tcaccacgag taatcacctt cactttcgag
    19681 gtcaggctct taaacgtagt cacaagaacc gccaccagta acgcgatcca ggcgatactc
    19741 agtatgggat gacggccaac aaattgcata atttcttgca tggggggtaa caactcccga
    19801 cgtagtgatt aaaaaaacca ggaaaggagt atacctgcgc ggtgcggcaa atacagccag
    19861 cgcgttaact ggaatgcaat tttgcggggc acgacgaaaa aaaagcgcaa atctggcatc
    19921 ggcttgccgc gagcggtcgt atattttgat cttcagaggc tattttatcg attcagctgt
    19981 agtaaaatta cgcaaatttt gactcttgag tatgaggttg tcgcaatgtc ggtttctaaa
    20041 aaacctatgg tactggtgat tctggatggc tatggctatc gtgaagaaca gcaggataac
    20101 gccattttta gtgctaaaac cccggtaatg gatgcactgt gggccaatcg tccgcatacc
    20161 ctgatcgacg cttccggtct ggaagtcggt ctgcctgacc gtcagatggg taactccgaa
    20221 gtaggccacg ttaacctggg tgccggccgc atcgtgtatc aggacctgac tcgtctggac
    20281 gttgaaatca aagatcgcgc tttctttgct aatccagtgc tgactggcgc ggtagataaa
    20341 gcgaaaaacg caggtaaagc agtacacatt atgggtctgc tctccgcagg cggcgtacac
    20401 agccacgaag atcacatcat ggcgatggta gaactggcag ctgaacgcgg cgcagaaaaa
    20461 atctacctgc acgcattcct tgacggtcgc gacaccccgc cgcgcagtgc tgaatcctcg
    20521 ctgaaaaaat ttgaagagaa atttgctgcg ctgggtaaag gccgcgtagc gtccatcatt
    20581 ggtcgttact acgcgatgga ccgcgataac cgttgggatc gcgttgaaaa agcctatgac
    20641 ctgctgactc tggcgcaggg tgagtttcag gccgatactg ccgttgctgg tttgcaggca
    20701 gcttatgctc gcgacgaaaa cgacgagttc gtgaaagcga ccgttattcg tgcagaaggt
    20761 cagccagatg cggcaatgga agacggcgat gcgctgattt tcatgaactt ccgtgctgac
    20821 cgcgcgcgtg aaatcactcg tgcattcgtg aacgctgatt tcgatggctt cgcgcgtaag
    20881 aaagtggtta acgtcgattt cgtgatgctg accgaatacg ccgctgacat caaaactgcg
    20941 gttgcttacc cacccgcttc cctggttaac accttcggcg agtggatggc gaaaaacgac
    21001 aaaactcagt tgcgtatttc cgaaaccgaa aaatatgccc acgttacttt cttcttcaac
    21061 ggtggcgtag aagagtcgtt caaaggcgaa gatcgcattc tgatcaactc accgaaagtg
    21121 gctacctacg atctgcaacc ggaaatgagc tccgcagagc tgaccgaaaa actggttgcg
    21181 gccatcaaga gcggcaaata cgacaccatt atctgtaact atccgaacgg cgacatggta
    21241 ggtcacaccg gggtgatgga agcggcggtt aaagcggttg aagcactgga tcactgcgtg
    21301 gaagaagtcg cgaaagcggt tgagtccgtg ggtggacaac tgctgatcac cgctgaccac
    21361 ggtaacgctg aacagatgcg cgatccggca acgggtcagg cacacacggc acacaccaac
    21421 ctgccagttc cgctgattta cgttggtgat aagaacgtga aagcggttgc aggcggcaaa
    21481 ctttctgaca tcgcgccgac catgttgtcg ctgatgggta tggaaatccc gcaagagatg
    21541 actggtaagc cgctgttcat cgtggaataa tccctcccca tgaggggaaa ggcgattaat
    21601 accatgacac gggccgtgaa accgcgcagg tttgcaatca ggcccatcat ctacgccagc
    21661 gttcttagcg ctggcgtatt gttgtgcgcc ttttccgccc acgcggatga gcgtgaccaa
    21721 ctcaaatcta ttcaggccga tatcgccaca aaagagcgcg cggtacgcca aaagcaacaa
    21781 caacgcgcaa gcctgctcgc acaattgaaa aagcaggaag aagcgatctc tgaagccacc
    21841 cgtaagctgc gcgaaacgca aaacacgctc aatcaactca ataaacagat tgatgagatg
    21901 aacgcgtcga ttgccaaact ggagcagcaa aaagccgccc aggagcgcag cctcgccgca
    21961 caactggatg ccgcattccg tcagggcgag cataccggta ttcagctaat tctcagcggt
    22021 gaagaaagcc agcgtggaca gcgtttacag gcttatttcg gctatctcaa ccaggcgcga
    22081 caagaaacca ttgcccagtt gaagcaaacg cgtgaagaag tcgccatgca gcgtgctgaa
    22141 ctggaagaga aacaaagcga gcaacaaacg ctgttatatg aacagcgcgc ccaacaggcg
    22201 aaactgactc aggctctgaa cgagcgtaaa aagacgctgg cggggctgga atcttccatc
    22261 cagcaaggtc agcagcagct aagtgaactg cgtgccaacg aatcccgtct gcgtaacagt
    22321 attgcccgtg cggaagccgc ggcgaaagcg cgtgctgaac gagaagcgcg tgaggctcag
    22381 gcagttcgcg acaggcagaa agaagcgacg cgcaaaggca ccacctacaa accgaccgaa
    22441 agcgaaaaat cgctgatgtc ccgaactggt ggcctgggtg cgccgcgtgg tcaggcattc
    22501 tggccagttc gtggccctac gctgcatcgc tatggcgagc agctacaggg tgaattacgc
    22561 tggaaaggaa tggttatcgg tgcctctgaa ggtactgaag ttaaagcaat tgccgacggt
    22621 cgggtgattc tggctgactg gctgcaaggc tacggtctgg tggtggtggt tgagcacggt
    22681 aaaggcgata tgagtcttta cggctataat caaagcgcgc tggtgagcgt tggttcgcag
    22741 gttcgtgcgg gccagccaat tgcactggtg ggcagcagtg gcggtcaggg cagaccttca
    22801 ctctatttcg aaattcgccg ccagggtcag gccgtcaatc cacagccgtg gttgggaaga
    22861 taagttttgt ttccatttcg tcgtaacgtt cttgcatttg ccgctctgtt ggcgctctcc
    22921 tcccctgtaa ttgctggcaa acttgccatc gtcattgatg attttgggta tcgcccgaac
    22981 aacgaaaacc aggtgctggc gatgccttcc gctatctccg tcgctgtatt acccgattca
    23041 ccgcacgcca gagagatggc gaccaaagcg cataacagcg gtcacgaagt gttgattcat
    23101 ctcccgatgg caccgttgag taaacagccg ctggagaaaa atacgctgcg cccggagatg
    23161 agcagcgacg aaattgagcg cattattcgt agtgcggtca ataacgtgcc ctatgccgtg
    23221 gggatcaata accacatggg cagcaagatg acctctaacc tgtttggtat gcagaaagtg
    23281 atgcaggcgc tggagcgtta taatctttac ttcctcgaca gcgtaaccat tggtaatacc
    23341 caggcgatgc gcgccgcgca aggcactggc gtgaaggtga tcaaacggaa ggtgttcctc
    23401 gacgattcac agaacgaagc ggacatccgt gtgcaattca atcgcgcaat tgacctagcg
    23461 cgtcgcaacg gatcaaccat tgccattggg catcctcacc cttcaacggt acgcgtgttg
    23521 caacagatgg tttataacct gccgccagac attacgctgg tgaaagccag cagcttgctg
    23581 aatgaaccgc aggttgatac gtctacaccg ccgaaaaaca ctgtgcccga cacaccgcgt
    23641 aatccgttcc gtggcgtgaa gctgtgcaaa ccgaagaaac cgatagaacc tgtttatgct
    23701 aatcgcttct ttgaagtatt aagcgaaagc atcagccaga gcacgctgat cgtttacttc
    23761 cagcatcagt ggcaaggctg gggcaaacag cctgaagcgg cgaagcttaa cgctagcgta
    23821 aattaaaggc gctacgcgta atgcgatggt gcgaccgcgt tttgtcgcgc cattgccata
    23881 agcggaaaga ctacagtaac gcctgataac ccacctttac gctgcgcaca ttggtaatca
    23941 ggcgcttata cata
//
LOCUS       DAEEKQ010000052        23732 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.052, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000052 DAEEKQ010000000
VERSION     DAEEKQ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23732
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.052"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(506..1303)
                     /locus_tag="KLK57_001957"
     CDS             complement(506..1303)
                     /locus_tag="KLK57_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0892327.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPASCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNSELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHVNCLLLSDSK
                     QSLISRCYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSILDDVRLNNA
                     LSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
     gene            complement(1313..1864)
                     /gene="ybcL"
                     /locus_tag="KLK57_001958"
     CDS             complement(1313..1864)
                     /gene="ybcL"
                     /locus_tag="KLK57_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HBE0892328.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGV
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(2033..2365)
                     /gene="emrE"
                     /locus_tag="KLK57_001959"
     CDS             complement(2033..2365)
                     /gene="emrE"
                     /locus_tag="KLK57_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HBE0892329.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(2699..3013)
                     /gene="fliE"
                     /locus_tag="KLK57_001960"
     CDS             complement(2699..3013)
                     /gene="fliE"
                     /locus_tag="KLK57_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HBE0892330.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            3229..4887
                     /gene="fliF"
                     /locus_tag="KLK57_001961"
     CDS             3229..4887
                     /gene="fliF"
                     /locus_tag="KLK57_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HBE0892331.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYCFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIETIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4880..5875
                     /gene="fliG"
                     /locus_tag="KLK57_001962"
     CDS             4880..5875
                     /gene="fliG"
                     /locus_tag="KLK57_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HBE0892332.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTYVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5868..6554
                     /gene="fliH"
                     /locus_tag="KLK57_001963"
     CDS             5868..6554
                     /gene="fliH"
                     /locus_tag="KLK57_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HBE0892333.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            6554..7927
                     /gene="fliI"
                     /locus_tag="KLK57_001964"
     CDS             6554..7927
                     /gene="fliI"
                     /locus_tag="KLK57_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HBE0892334.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7946..8389
                     /gene="fliJ"
                     /locus_tag="KLK57_001965"
     CDS             7946..8389
                     /gene="fliJ"
                     /locus_tag="KLK57_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HBE0892335.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            8386..9513
                     /gene="fliK"
                     /locus_tag="KLK57_001966"
     CDS             8386..9513
                     /gene="fliK"
                     /locus_tag="KLK57_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HBE0892336.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALASETTTD
                     KAAPQVLVATDKPTTKGELLISDIVSDAQQADLLIPVDETLPVINVEQSTSTPLTTAH
                     TMTLAAVADKNTTKDEKADDLNEDLTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLLTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEEDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            9618..10082
                     /gene="fliL"
                     /locus_tag="KLK57_001967"
     CDS             9618..10082
                     /gene="fliL"
                     /locus_tag="KLK57_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HBE0892337.1"
                     /translation="MTDYAISKKSKRSLWLPILVFITLAACASAGYSYWHSHQVAAED
                     KAQHRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVMYTAFILR"
     gene            10087..11091
                     /gene="fliM"
                     /locus_tag="KLK57_001968"
     CDS             10087..11091
                     /gene="fliM"
                     /locus_tag="KLK57_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HBE0892338.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGNGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDGIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            11088..11501
                     /gene="fliN"
                     /locus_tag="KLK57_001969"
     CDS             11088..11501
                     /gene="fliN"
                     /locus_tag="KLK57_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HBE0892339.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLISQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            11504..11869
                     /gene="fliO"
                     /locus_tag="KLK57_001970"
     CDS             11504..11869
                     /gene="fliO"
                     /locus_tag="KLK57_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HBE0892340.1"
                     /translation="MNNHATVQSSTPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11869..12606
                     /gene="fliP"
                     /locus_tag="KLK57_001971"
     CDS             11869..12606
                     /gene="fliP"
                     /locus_tag="KLK57_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HBE0892341.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     SVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            12616..12885
                     /gene="fliQ"
                     /locus_tag="KLK57_001972"
     CDS             12616..12885
                     /gene="fliQ"
                     /locus_tag="KLK57_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HBE0892342.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12894..13679
                     /gene="fliR"
                     /locus_tag="KLK57_001973"
     CDS             12894..13679
                     /gene="fliR"
                     /locus_tag="KLK57_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HBE0892343.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFVIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEMFNLLADIISELPLI"
     gene            13964..14587
                     /gene="rcsA"
                     /locus_tag="KLK57_001974"
     CDS             13964..14587
                     /gene="rcsA"
                     /locus_tag="KLK57_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HBE0892344.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQHIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKKLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(14631..14819)
                     /gene="dsrB"
                     /locus_tag="KLK57_001975"
     CDS             complement(14631..14819)
                     /gene="dsrB"
                     /locus_tag="KLK57_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HBE0892345.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14982..15209
                     /gene="yodD"
                     /locus_tag="KLK57_001976"
     CDS             14982..15209
                     /gene="yodD"
                     /locus_tag="KLK57_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HBE0892346.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            15507..16322
                     /locus_tag="KLK57_001977"
     CDS             15507..16322
                     /locus_tag="KLK57_001977"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HBE0892347.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPTALWLSRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQDIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIVKGLNREGVHLHDEDPTRVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(16319..18013)
                     /gene="yedQ"
                     /locus_tag="KLK57_001978"
     CDS             complement(16319..18013)
                     /gene="yedQ"
                     /locus_tag="KLK57_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HBE0892348.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLSVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNTSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFIGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSNNYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRFLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMDSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     SLQWQAWHDTLTRLYNRGALFEKARPLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTEAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVCASDNA"
     gene            complement(18184..18366)
                     /locus_tag="KLK57_001979"
     CDS             complement(18184..18366)
                     /locus_tag="KLK57_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HBE0892349.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(18445..19362)
                     /locus_tag="KLK57_001980"
     CDS             complement(18445..19362)
                     /locus_tag="KLK57_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HBE0892350.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            19535..20456
                     /gene="yedA"
                     /locus_tag="KLK57_001981"
                     /pseudo
     CDS             19535..20456
                     /gene="yedA"
                     /locus_tag="KLK57_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
     gene            complement(20445..20915)
                     /gene="vsr"
                     /locus_tag="KLK57_001982"
     CDS             complement(20445..20915)
                     /gene="vsr"
                     /locus_tag="KLK57_001982"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HBE0892351.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGSPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRGREKLTDEALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20896..22314)
                     /gene="dcm"
                     /locus_tag="KLK57_001983"
     CDS             complement(20896..22314)
                     /gene="dcm"
                     /locus_tag="KLK57_001983"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HBE0892352.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPSAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(22381..23037)
                     /locus_tag="KLK57_001984"
     CDS             complement(22381..23037)
                     /locus_tag="KLK57_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HBE0892353.1"
                     /translation="MDLQHWQAQFENWLKNHHQHQDAAQKLAADDDVDMLVILTACYF
                     HDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCHAIAAHSFSAQIAP
                     LTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQHRPLDDKRYALDH
                     FQTKLLKLPQTMQTARGKQLAQHNAHFLVEFMAKLSAELAGENEGVDHKVIDAFSPAG
                     "
     gene            complement(23077..23442)
                     /locus_tag="KLK57_001985"
     CDS             complement(23077..23442)
                     /locus_tag="KLK57_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBE0892354.1"
                     /translation="MEKCDFYHIIVLSLNFPGYLKMEYGSTKMEERLSRSPGGKLALW
                     AFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWLGALVGFLFMALVGAL
                     LGSIAWYTRPRPARSRRYE"
BASE COUNT         5903 a         6149 c         5903 g         5777 t
ORIGIN      
        1 atctacttca caatttaaat gagaaactaa aacttacatc ttgaaataat ctcattgatt
       61 ggatgaatat ttgtagtgca gtgacatcat ttttaaataa tagttcaaat aaaagggctc
      121 acgatgaaaa aattaacagt ggcaatttct gctgtagctg catcttaacg tataaactag
      181 atttaataat aacgaactag atttaataac aacgatatcc gtacctatcc ggatatctaa
      241 tgtcataagc atccctttaa ttatcccctc aacctcttgt aactttccca ctcttgaact
      301 ccacatagtc caaacgccgg atgcaaaaaa atcacacgtg catttctgaa attgtgatgg
      361 aggaagatag tatcaattat atgtgcttat aaacgtcccc gattagcgca gaagtcaccg
      421 gattgttcag gctccaatag gttgagtatg ccgtatttat tttccaaaat aaaaatcgat
      481 gctgttgctc cttcataaaa tacttttact ctctggacgc ttttcttagc tctcttggtg
      541 ttatcttaaa acgattatgg aacctttcag taaaacgaga aggacacttg taaccatttt
      601 ccctggcaat ctcgctgatg ggttttactg tcgtttgtat agcagacaac gcattattta
      661 acctcacgtc gtccaatata ctttgaaaac ttactccctc gcttgccaga cggcgatgca
      721 atgtggaaac agaaatgtag agatatctcg ctaccttgtt tgctgtccat tttgttccag
      781 gttcggatag cattaggtta taacaacgac ttattaatga ttgtttacta tctgataaaa
      841 gtaaacaatt aacatgatgt gctcctaatg aaagtagaac gcccattgct aagtgttcct
      901 gaatttgcgt tgagaagcct cgggaaatag atgtttttag ttgctcccaa caatatatta
      961 actcggaatt ctgaggtaag cagaaacctg tcgtgttacg aatttgatcg gttatcgcat
     1021 aaagattttg gaacttttca attagatcaa tgggataata aagcatttct gcaagataaa
     1081 gacctgcttc tgggtaattc gcaatataaa attcatatcc acagggaaat aatattatct
     1141 gattattatc aacagttaga ctatgcgtct cccaattgat aacttttttt ccctgacgga
     1201 tacgacatga agctggcata agaggcttaa ccctgtgaat ctcatgatgt ttatgcatcc
     1261 gtatttcttc aatctttagg ttagtcctat cttttgccag catactctta tcctacttta
     1321 cctcataaac gggtattatc tcagcggttg caattttatt ggcattaagc atataaccaa
     1381 ctaacgctcc gctggaatta gaatccacag gaatcttatc agtttttaga gcccatattt
     1441 taaactggta atgatggggt ttatctcctt tgggaggaca tgctccacca aatcccgcat
     1501 agccaaaatc atttcggcct tggacagcac cagtcggaag ttttgtacca tcacgtcttc
     1561 ctgcatcagc gggcaaatat gttaccgttg ctggaatatt agcaacagtc cagtgccacc
     1621 atccgctgcc tgtaggcgca tctggatcat acacagttac ggcaaagctt ttggtgcctt
     1681 ctggagcacc agaccaggtt aatgagggcg atgtattacc cccctcacac ccaactccag
     1741 aaaatacatg agacgttgta agttgctctc ctgtttttat ttcattacta gtgacctgaa
     1801 atgctgcggc ctgcgcagaa aatgttatga atgccaatac acttgaaacg ataagttttt
     1861 tcataaaaac ctctttgttg tgacctatcg ttattttatt tgatgtgcat tgatctcatt
     1921 atgcaaaaag gcgcaatgat cgcgcaaatg caatcataat tataaagttg ccccgaacat
     1981 tagccataat atgcacaaat cagattcctg ttatctcaag gatgattatt ttttaatgcg
     2041 gtgcgcttcg tgacaacaag ttaatgacta acacaccggc acaaatcaac atcatgccta
     2101 tgatggctgg caggtccagt cgttggccaa aaaatcccca ggaaagcaaa ctaatcagaa
     2161 cgataccgac tcctgaccag atagcataag caatccctgt aggtatataa gccagtgtct
     2221 gagctaataa ccaaaatgat gcacaatagc aaataattgt gccaacagag ggccataacc
     2281 gtgtaaaccc ctctgaaaac ttcattaagg ttgtaccaat gacctctgca agtattgcgc
     2341 caccaagata aatataaggg ttcatagcat attctttcct gttcatactg gagagaattg
     2401 tactacagtt tgaacacaac tcaccgtttt catcctggtg tatgtgtatc cagggggctc
     2461 tggcgtggca agagataaag gcaggaacaa taatgatcag aaggaacccc aggctaacaa
     2521 aagagttcac tcttcctatg agcaaagcga agacaaaaca tgttatcgcg tttacggatc
     2581 gtggatggca gaaaactttc aggatcaggt atccatttta aaccagaaat tgagtgagtt
     2641 tgccccatcc atgccccacg cggtgagatc ggatgtaatt aacaacaggt tacaaaacct
     2701 acacctgcat ggacatcact tcctgataag ccgccaccag cttattacgc acctgaatcc
     2761 ccatttgcat agaaactgag gctttttgca tatcggtcat cacatcgttt aacgccacgc
     2821 cgggttcacc gagagtgaat ttttctgcct gcgtgcgggc ggctgtttgt gtatcgctta
     2881 tgcgatcgag cgcggcgtgc agctgcccgg caaaactaat ggtcggttgt ggcagtgatt
     2941 cctgcgcacg cgcactcatc gccgtagcct gtaactggct gataacccct tcaatcccct
     3001 gtatcgctga cattttcatc tcccggataa tttctggtag caaagcttac cagtaagtca
     3061 ataagacaaa ggcgctaaat agcaacaaaa aaaacgggtt tattggcgga tagaaaaaaa
     3121 cgaaagcaca aataatggca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt
     3181 gttccacttt gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact
     3241 gcagcccaga caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca
     3301 ttgattgttg ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa
     3361 gcccccgact accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc
     3421 agccaactga cgcaaatgaa tattccttac tgcttcagcg aagccagcgg cgctattgaa
     3481 gttccggcag ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa
     3541 ggcggcgcgg tcggtttcga actgcttgat caggaaaagt ttggtatcag ccagttcagc
     3601 gaacaggtga attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgaaactatc
     3661 ggcccggtaa aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt
     3721 gaacaaaaat ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat
     3781 gaagggcaaa ttagcgccat tgtgcacctg gtttccagcg ccgttgctgg tctgccgccg
     3841 ggaaacgtca cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg
     3901 cgcgatctta atgacgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg
     3961 cgtattgaag caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg
     4021 gcgcaactgg acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat
     4081 gaatctcatg cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt
     4141 tatccgggcg gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca
     4201 atcagcacgc ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat
     4261 agcgggccgc gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt
     4321 cgtcatacca aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat
     4381 tacaaaacct tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt
     4441 gaagatctga cccgcgaagc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc
     4501 gttaactcgc cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag
     4561 caagcgttta tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg
     4621 tggctgctgt ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa
     4681 gctgtacagc aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg
     4741 agcaaagacg aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg
     4801 agccagcgta tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc
     4861 cagtggataa ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct
     4921 gatgaccatt ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt
     4981 gcaaaccctg agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccta
     5041 tgtgctggcg gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa
     5101 cgattatctg cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct
     5161 ggaagatatt ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga
     5221 gccgcagagt gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct
     5281 ggtgcatctg aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg
     5341 ccacgacgtg atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga
     5401 actgaccgaa gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg
     5461 cggcgtgaga acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt
     5521 tattaccgcc gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt
     5581 cctgttcgaa aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt
     5641 ggattccgaa tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt
     5701 cctgcgcaat atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg
     5761 tccggtgcgt ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg
     5821 ccttgccgaa actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc
     5881 tgccgtggaa aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca
     5941 tggtcgagcc ggaagaaacc atcattgaag aggccgaacc tagccttgag cagcaactgg
     6001 cgcaactgca aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc
     6061 agcaaggtca tgagcagggc tatcaggaag gactggccca ggggctggag caaggtctgg
     6121 cagaggcgaa gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat
     6181 ttcaaactac ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc
     6241 tggaggcggc acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca
     6301 aacagatcca acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg
     6361 tgcacccgga tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg
     6421 gctggcgctt gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg
     6481 aaggcgatct cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac
     6541 caggagtggt gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga
     6601 agccaaaatg gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg
     6661 gctggtgctg gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg
     6721 ccagaacggc agcgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg
     6781 gctgttttta atgccgctgg aggaagtgga aggtgtcctg cccggcgcgc gggtttatgc
     6841 caaaaacatt tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt
     6901 attaggtcgc gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac
     6961 gacggaaacc ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga
     7021 acatgtgctg gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca
     7081 gcgtatgggg ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc
     7141 acgttacacc cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt
     7201 aaaagatttt attgagaata tcctcggtgc tgaagggcgt gcacgctccg tggtgattgc
     7261 cgctccggcg gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat
     7321 tgccgaagat tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg
     7381 ctacgcgatg gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg
     7441 ttatccaccg tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat
     7501 tagcggcggc ggctcgatta ccgcgtttta taccgtgctt actgaaggcg atgaccagca
     7561 ggaccccatt gcagactccg cacgggcgat cctcgacggt cacattgtgc tgtctcgccg
     7621 actggcggaa gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat
     7681 gacggcgttg atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc
     7741 gagttttcag cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc
     7801 gatgctcgat aaagccatcg ccctgtggcc gcagctggag ggctatttac aacaaggcat
     7861 ttttgaacgt gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt
     7921 gtcataaccc aggagataac ggtagatggc agaacatggt gcgctggcga ccctgaaaga
     7981 tctggcagaa aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcgggtg
     8041 tcagcaggcg gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa
     8101 cctcaacagc gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt
     8161 tatccagacg ctggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca
     8221 gaaagttgac attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca
     8281 gacactgcag gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa
     8341 aaagatggat gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg
     8401 cccttgatta ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct
     8461 gcacaagatt ttctcgcgtt gttgagcgaa gcattagcaa gcgagacaac taccgacaaa
     8521 gcggcccccc aggtgctggt ggcaacagat aagcccacga caaaaggcga gctgctgatc
     8581 agcgatattg tttccgacgc gcaacaagcg gatttactga tccctgtgga tgaaacactg
     8641 cctgtcatca acgtcgaaca atccacatcg acaccgttaa ccaccgctca tacgatgacg
     8701 ttggctgcgg tggctgacaa aaatacgaca aaagacgaaa aagcggatga tctgaatgaa
     8761 gacctcaccg caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc
     8821 aaagtgactg atgcgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa
     8881 ctgacttctg cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag
     8941 ccattaacac cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca
     9001 ccggtgaccg ctgccgccag cccgctaatc actccacacc agacacagcc actgctcacc
     9061 gtcgccgcgc ctgtcttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc
     9121 cagcatattt cgctgttcac ccgccaggga caacaaagtg cagagttgcg tctgcacccg
     9181 caggatttag gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag
     9241 atggtttcgc cgcatcagca cgtacgcgcc gccctggaag cagcgctgcc ggtactgcgt
     9301 acgcagctgg ccgagagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt
     9361 agtggtcagc agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa
     9421 cctctggcgg gggaagaaga cgatacgctt ccggttcccg tctctttaca agggcgcgta
     9481 acaggcaaca gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt
     9541 cttttccccg ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca
     9601 caggaagacc gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt
     9661 ggctcccgat tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact
     9721 ggcattcgca tcaggttgcc gctgaagaca aagcgcagca tcgcgtcgtg ccctcaccgg
     9781 tcttctatgc gctggatacc tttacggtca atttgggcga tgcggatcgc gtgctttata
     9841 tcggtataac cctgcgactc aaagatgaag ctacccgctc gcggctgagt gagtatttgc
     9901 cggaagtccg tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga
     9961 cagaagaagg caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg
    10021 ttgccgggca accgaaacag gatgtcaccg acgtgatgta taccgctttt attctgcgat
    10081 aacgacatgg gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac
    10141 agcgaagtca aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac
    10201 gatccgaata cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat
    10261 gagcgctttg cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat
    10321 ataaccgtcg gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg
    10381 ccgaccaacc tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt
    10441 tcaccgagtc tggtgtttat cgccgtggat aacctgtttg gcggcaatgg acgcttcccg
    10501 accaaagtgg aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta
    10561 aagctggcgc ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag
    10621 tacgtgcgtt cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt
    10681 gtggttaaca cgccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt
    10741 ctgccattca gcatgatcga gccgctacgg gaattgttgg ttaacccgcc gctggaaaac
    10801 tcgcgtaatg aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag
    10861 ctggagctgg tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg
    10921 aaacccggcg acgtcctgcc gatagaaaaa cccgatggca tcatcgccca tgttgacggc
    10981 gtcccggtgc tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa
    11041 catttgatta acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga
    11101 ataatccggc cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg
    11161 aacaaaaatc aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg
    11221 gtgatgtcag cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga
    11281 ccgtcgagct gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt
    11341 ccgtcgtggc gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt
    11401 taatctccca gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata
    11461 tcattactcc gtctgagcga atgcgtcgcc tgagccgtta gtgatgaata accacgctac
    11521 tgtgcaatct tccacgccgg tttctgctgc gccactgctg caggtcagcg gcgcactcat
    11581 cgccattatt gccctgatcc tcgctgctgc ctggctggta aaacggttgg gatttgcccc
    11641 gaaacgcact ggcgttaacg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga
    11701 acgggttgtg gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca
    11761 aatcaatctg ctgcataaac ttcccccttc agcaccaaca gaagagatac cgcagaccga
    11821 ttttcagtcg gtcatgaaaa atttgcttaa gcgtagtggg agatcctgat gcgtcgtttg
    11881 ttgtctgtcg cacctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgccg
    11941 ggtatcacca gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg
    12001 ctggtgttca tcacctcgtt gacgtttatt ccggcaattt tactgatgat gaccagtttc
    12061 acccgcatca tcattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca
    12121 cctaaccagg tattgctggg gctggcacta tttttgacct tttttattat gtcatcggtg
    12181 atcgacaaaa tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag
    12241 gaggcgctgg aaaaaggggc gcagccgcta cgtgagttta tgctgcgtca gacccgtgag
    12301 gcagatttag ggttgtttgc cagactagcg aataccggcc cgttgcaggg acctgaagcc
    12361 gtgccgatgc gcattttgct cccggcctac gtgaccagcg aattgaaaac cgcatttcag
    12421 ataggcttca cgattttcat tccttttttg attatcgacc tggtgattgc cagcgtgttg
    12481 atggcattgg ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg
    12541 ctgtttgtac tggtggatgg ctggcaattg ctggtcggtt cgctggcgca gagcttttac
    12601 agctagagag gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa
    12661 gtcgcgctgg cactggctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc
    12721 agtattttgc aggccgccac gcagatcaac gaaatgacgc tgtcgtttat tccaaaaatc
    12781 atcgccgtat ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat
    12841 tacgtccgca ccttgttcac taacctgccg tatatcatcg ggtaggccgt acaatgatgc
    12901 aggtgacaag cgatcaatgg ctttcctggt taagcctgta cttctggccg ttactgcgcg
    12961 tgctggcgct catttctacc gcaccaattc tgagcgaacg cagcgtgccg aaacgggtaa
    13021 aactggggct ggcaatgatg atcacgttcg tcattgcccc atcattacct gccaacgatg
    13081 ttcctgtttt ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcattg
    13141 cgcttggttt taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg
    13201 gtctgcaaat ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc
    13261 ccgttttagc gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc
    13321 atttatggtt gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac
    13381 cgttgaacag caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg
    13441 ggctgatgct ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt
    13501 tactgaatcg tatggccccg caattatcca tttttgttat tggatttcca ttaactctga
    13561 ctgtcggtat ctctttaatg gcggcattaa tgccgttaat tgcacctttt tgcgaacatt
    13621 tattcagtga gatgtttaat ttgctggctg atattattag tgaattgcca ttaatataat
    13681 tccgtaacgt ttatcatgtt atcctaagga ttatccgaaa aataatacct acgaacatct
    13741 tccaggatac tcctgcggcg aaatatttgt tttaagctca ctcacatatc gcaacattta
    13801 ctttaagaca attccaggca aattatacaa cactttacgg gatagtaagt ccgcctgaaa
    13861 aatcgcgaga gtggcgcatt aggtgaccca tgttgttccg tttagtcatg atgaaatatt
    13921 caggtaaggg gaattatcgt tacgcattga gtgagggtat gccatgtcaa cgattattat
    13981 ggatttatgt agttacaccc gactaggttt aaccgggtat ctgttgagta gaggggttaa
    14041 aaaaagagaa atcaacgaca ttgaaaccgt tgatgacctt gccatagctt gtgattcaca
    14101 gcgcccttca gtggtgttta ttaatgagga ctgtttcatc catgatgctt ctaacagtca
    14161 gcacatcaag cacatcatta atcaacatcc caatacgtta tttatcgttt ttatggcaat
    14221 tgccaatgtt cattttgatg aatatctatt ggtcagaaaa aaattattga taagttctaa
    14281 atcgattaaa ccggaatctc tcgacgatat ccttggcgat attctgaaaa aagagacaac
    14341 gataacctct tttttaaata tgccgacgtt atcattgagc cgaaccgaat cgagtatgtt
    14401 gcgaatgtgg atggcaggtc agggaaccat tcaaatctct gaccaaatga atatcaaagc
    14461 caagaccgtt tcatcgcata aaggtaatat taaacgtaag atcaaaacac ataataaaca
    14521 ggttatctac catgtcgtcc gactgacgga taatgtgact aatggtattt ttgtcaacat
    14581 gcgctaacac attctgactg gtggtttccc accagtcagg ctgaataaga ttactctgct
    14641 ttctccacaa agataccgtc ctgatgccct gcttcattaa agaaccagat gccgagcggg
    14701 tagtcttcca gcgaaaccag gtacattgtg ccttcactaa actcctcaac tgccagtacc
    14761 acgccaggac gacgcggacc gccatccgtt ttgactgtta cccgatcatt caccttcatc
    14821 gttttcctcc tgtggctttg tgccagtgta gaacaatttc gtttttctgg cagctccagg
    14881 cgcgcgcgag tgctgatttt ctcgacggtc tatacttaag agatgccagg cggacttaac
    14941 gactggcggc aacaacagag taacggttgc gaggaaagat gatgaaaacc gcaaaagagt
    15001 acagcgatac cgcaaaacgt gaggtcagcg tcgatgtcga tgccctgctg gcggcgatca
    15061 atgaaattag cgaaagcgaa gttcatcgca gccagaacga ttctgaacac gttagcgtcg
    15121 atggacgtga atatcataca tggcgtgaat tggcggatgc cttcgaactg gatattcatg
    15181 acttcagcgt ctctgaagtg aatcgttgaa tgcacaataa aaaaatcccg accctgaggg
    15241 ggtcgggatg aaacttgctt aagcaagaag cacttaaaaa attcgttaca ccaggaaatc
    15301 tgatgtgttc atcaccttat ccgcaatttt tttcgctgac aagaaaatat tcgcgatatg
    15361 aataattaac gctacttatg aatagaaatg tgacgtgcgt caccccttgg tggctctggc
    15421 gtcaacggga tcgctcccga aaaaatcatc tttgattatt tgtgctgttt tgagaatcat
    15481 cctggtaagg ggagaactgc acgaccatgt tttcaattca acaaccacta ctggttttta
    15541 gcgatcttga tggcaccctg ctggacagtc atagttatga ctggcaaccg actgccctct
    15601 ggctcagccg tttacgcgaa gcgaatgttc ccgtcattct ctgtagcagt aaaacttcag
    15661 cggaaatgct gtacttgcaa aaaacgttgg ggctacaagg gttaccactg attgccgaaa
    15721 atggcgcagt gatccagctt gctgaacaat ggcaggatat agacggtttt ccacgcatca
    15781 tctcaggtat tagccatggc gaaatcagcc aggttttaaa tacgctacgc gagaaagaac
    15841 attttaaatt cacgactttc gatgatgtcg acgatgcaac catcgccgaa tggacgggat
    15901 taagccgtag ccaggcggcg ctgacgcagc tgcatgaggc gtcggtaacg ctaatctggc
    15961 gcgacagtga cgagcgtatg gcacaattta ccgctcgtct gaacgaactg ggcttacagt
    16021 ttatgcaggg tgcgcgcttc tggcacgtcc tggatgcctc tgccggaaaa gatcaggccg
    16081 ccaactggat tatcgcgacc tatcaacaat tatcaggcaa acgcccaacc acacttggcc
    16141 tgggcgatgg gccaaacgat gcgcccttac tggaggtaat ggattacgcg gtgattgtga
    16201 aagggctaaa ccgtgaaggg gtgcatctgc atgatgagga tccgacccgc gtctggcgaa
    16261 cgcagcgtga aggaccggaa ggctggcgtg aagggctgga ccattttttc tccgctcgtt
    16321 aagcgttatc gctcgcgcat acccgattac gcccggcctg tttagcgaga taaagccgac
    16381 ggtcagccag tgactggagt tgttcaaaat cataatcacc ggtttcctcg ctgctactta
    16441 cccccaacga ggcactgatg cgtatcgtcg tactcttagc aatcaacatc tctttttcat
    16501 ttaacttcag gcgaatacgt tctgcgactt ccgcagcctc cgtcagactc gcgcctggca
    16561 gaatcacaca aaactcctca ccaccgaccc gcccggcaac gtcctgcgca cgcaaggaac
    16621 tgctaattaa tccggcagca tgagaaagaa cacggtcgcc cgcctgatga ccaaagcggt
    16681 cattaatcgc tttaaaatgg tcaaggtcga cctgaatgac agaaaaagga tgttggtgcg
    16741 tctgacacaa tttcgcgagc ggacgggctt tttcaaacag tgcgccacga ttatataaac
    16801 gggttaaggt gtcgtgccac gcctgccact gcaacgagct ttgcagaaca tacatgttgc
    16861 taaccatccg gcgaatcaca taccaggaga tgagtaacat ggtggtaaag agcgcccaca
    16921 gcagggttaa tgcaatgctg atactgccga aatcgccgcg cacgccttcg cttagcgtat
    16981 ggacgcgcac cagcacaccg tcgaaatggt ccagacgttc ccagctaaca tagcgactgt
    17041 ccatacgaat gccgccccgc gtgtcatgtt ccatcgcctg cgccagcaag gccagttcac
    17101 gaggatcaaa aatattccct gttggatggt caggattgga agaggtcaaa aatctcagct
    17161 tactgtcata gagctgatac tcaccatcga ggtttttatc gatggcgttt cttaaaaatt
    17221 gctgcatggt acgcacggga atactcatcc ccagcacgcc ataccagtag ttattactgt
    17281 ctaccggaac actgacggta acctgcggtt cagtattgct ggcgtgctcc ggttgcgaag
    17341 taaaccagcg taccgcgcgg tgacgatttt ctcgttgcga atggccgata aaccagggtt
    17401 gggtgacata gccgtaataa cgcgttggta cattgcgcgt aaacaaggtc ggctgcgtcg
    17461 aaacgtaaaa tccggcacgc gagacataca tcgcctgttc aaccatcgac gaggtgttgt
    17521 gcgccagtcg cagcaagtaa ccaacttcca gtgcagcggt aatttcattg tcgaggcttt
    17581 cattttcgcg agacaggaga ttcccctcgc tgactaatgc atccgatacg ccattgactg
    17641 acagggtgcg tcgtcggttg agttcaattt gccaggcgtg ctcgtcgcga tgctgttcga
    17701 actcggttac agcattacgc agtgaagtga aatccagtgg cgctacgaga gcttcgcgca
    17761 tgccattacg caaaaagatc agtttgtcga cgttatactg caaatgctta tcgagcgcgt
    17821 tggctacgtt ttccagatga ttacgctggc tggagatata ggcatcttcc agcactacca
    17881 cttcacgcca ggtcagcaag gtggaaaaaa gcaataccac gataaagcag agattaacga
    17941 catgaccagg ccccaggcgg cgtgcgagtt ttttcaacca gctctggttt tccatttttg
    18001 tctcgtgctg caccctgacg actcccaacc tgctttttta tgattctggc ataacctggc
    18061 gatagcgtac cgcaaaacag cgataatttt ataatggtgg catgataaga cgcgactatc
    18121 attcattgca ggcattggca caccaggccg gaagtgatcc ggcctggtgt taatgatacc
    18181 ctttcaaact tcttccgcag aacgactccc ctcccccgga acaagctcgg tttcatgaaa
    18241 agcttcccgc ttgaccccgt aaccgtcata ccaacgacac tcaaccatac cgctggagta
    18301 tccagtgaca atcatccgcg ggccgccctc tttaaccgta acttcctcac taaccataaa
    18361 gctcatactc gcctcctttt ttcgagtgaa acttgttcac cttagttgaa gatggcgaat
    18421 tttgcataat agcctgtacg agtattaatg tgcctgaccg cgcattttcg ctacggcttt
    18481 cactcccagc accacgatgc cgccgatgat aaatccaaga atcagattta aaacagtcgg
    18541 taatatcatc gccaccactg cactttgctg cccggcgaaa tgttcaatgg cgtgatgcag
    18601 cggcgcaata ccatgtacca caataccgcc gccgacgagg aacatcgcca gcgtgccgac
    18661 aatcgataac gccttcatca accagggcgc aatgatcaat aaccctttac ctaatgcctg
    18721 catcagcgcg ctggattttt ccgccagcca ataccccagg tcatcaatct taacgataac
    18781 ccctaccaga ccgtagacgc ccacggtcac taccagcgcg atgcctgaaa gcaccagcac
    18841 ctgattaagc aacggcgctt cggccacaat ccccagcgtg atggcgacga tttcggcaga
    18901 caatataaaa tcggtacgaa tcgccccttt tattttgtcc ttttcaaact tcagcggatc
    18961 ctgcgccgcc agcttctcca gacgctgctg gctctgcgcc ggatcttctt tatgtttacg
    19021 cgcctccagc atatgcagca ctttctctac tccttcaaag cagagaaacg cgccaccaat
    19081 catcaacaga ggcgtaatcg cccacgggat aaacgcactg atgatcagcg ccagcggcac
    19141 cagaatcact ttattaatca gcgatccttt cgccacgccc cagaccacgg gaagttcccg
    19201 gttggcccgc acgcctgaaa cttgttgcgc attgagcgat aagtcatccc ctaatacacc
    19261 ggcggttttc ttcgccgcca gtttgcccat cacggagata tcgtccagta gtgtggcgat
    19321 atcatcgagc aacgtcagta aactactccc ggccaaaagc attccctctc atttttattg
    19381 ttgaagggtg taagtatgaa gcaaaaacgg cagctctgat acttgcttca caggtcaaca
    19441 ttttttttac aatcaagtgc aattatatct ctcgccagca gaatgatttt cacgtttact
    19501 atgaaccacc tttttatttc caccgtgagg gattatgcgt ttccgccagt tgttaccgct
    19561 ttttggcgcg ctgtttgcgt tgtatatcat ttggggctca acctattttg tcattcggat
    19621 tggcgtggaa agctggcctc cgttaatgat ggcaggcgtt cgattcctgg cagccggtat
    19681 tttattgatg gcatttttgc tactgcgcgg acacaagctc cccccgctac gtccgctgct
    19741 caatgccgcg ctgattggcc tgttattgct ggctgtcggt aatggcatgg tgacggttgc
    19801 cgagcatcaa aatgttcctt ccggcatcgc cgccgttgtg gttgcaaccg tgcccctctt
    19861 taccctggtg cttcagccgc ctgtttggca ttaaaacgcg caaactggaa tgggtgggta
    19921 tcgccattgg gcttgccgga atcatcatgc tcaatagcgg tggaaattta agcggcaatc
    19981 cgtggggcgc gattctgatt ttaatcggct cgattagctg ggcgtttggc tcggtttatg
    20041 gctcgcgcat taccttacct gtagggatga tggcgggtgc gattgagatg ctggcggcag
    20101 gcgtggtgtt aatgatcgcg tcgatgattg caggtgaaaa actgacggcg ctcccttccc
    20161 tttcaggctt ccttgcggtc ggctatctgg cgctgtttgg ttcgattatc gccatcaacg
    20221 cttatatgta tttaatccgt aatgtcagtc cggctctcgc caccagctac gcctacgtta
    20281 acccggtggt cgcggtcttg ctgggtacgg gactgggcgg agagacgctg tcgaagattg
    20341 aatggctggc gctcggcgta attgtcttcg cggtggtact ggtcacgttg ggaaaatatc
    20401 tcttcccggc aaaacccgta gttgcgccag ttattcagga cgcatcaagc gagtaaatga
    20461 atcccctgcg tgtcgatctg cgcgctggca ccttcgccgc agatccactc ttccagacgc
    20521 tcggtaagcg cttcatccgt cagcttctcg cgcccacgta atgcacactc ccagacaatc
    20581 agtacgcgcc agccgagttc ctgcaaacga ctgatatcgc ggcgatcgcg ttcaacattt
    20641 ttacctatct tctccagcca gaactcggtt cgagtcgcag gcactttaaa cagatagcag
    20701 tgatgatgat gccagaagca gccatgggta aatatcacgc agcgatattc atcaacgaca
    20761 aaatccgggc ttccgggcag actggcgtcc tgaacgcgaa acgccaggcc ctgcccggtt
    20821 aacagactgg cgaggcgctt ctcaatcgcc gtatcacgcg tggcaatcgc gcgcatattt
    20881 ttgctgcgag tggccttatc gtgaacgtcg gccatgttgt gcctcttgct gacgcaacgc
    20941 caccgcctgt ttgatttttg gctcaagcag ttttgccacc gcggcaaaga ccggcacgac
    21001 caccgagtta ccgaactggc gataggcctg agtgtccgaa accggaatgc ggaatttcgc
    21061 ttctcccggc gcttcaaaac ccattaagcg cgcgcattcc cgaggcgtta accgacgtgg
    21121 acgatgttgc tgattcagcg gatcgtcaaa atctttctca cccgtggcca tatcccagcc
    21181 gcgatcgatt aaaatttccg cgccatcttt gtaataacgc gcagacagcg tacgcgtgac
    21241 gctttgcgga ttgttcggat aaaccattcc ataaccgaag ccgttaccgc gcgcctgatg
    21301 ttttttcgca tatcgataga ggtacttcca cagcaccggc gtcaggatat atttcgcctc
    21361 gaccatcggg tccaacagct gcgccagcgt cactcgctgt gcagggaaac attcgctgat
    21421 atcacgcaga gtaaaatcgg ctttcagatt aagatcgcga cgaaaaccca ccagcacgat
    21481 gcgttcacgg tgctgcggca gaaaatgttt accatcgatg attttcggat cgtccggccc
    21541 gttatcttct gcatcagcca cgtcatagcc cagttcgtcc agcgtctgca tgatgatgcg
    21601 gaacgtttta ccctggtcgt gacttttcag gtttttgacg ttttcgagca caaacatcgc
    21661 cggacgacgc gcgtcgataa tgcgtaccac atcaaaaaac agcgtgccct gggtatcgca
    21721 ggcaaaaccg tgcgcccgcc cgagcgagtt ctttttcgat acgccagcca gcgaaaatgg
    21781 ctgacaaggg aaaccggcca gtaaaacatc gtgttcagga atgtgttgac gaatatgttc
    21841 cgccgccgcc tcatcactca cgccttcttt atggctgagg gtgatgtcgc ggatatcttc
    21901 attaaaatga tgcgtcgccg gatcgcaata atggttggct ttataagtgc gtaccgcatg
    21961 tttgttccat tcgctggtaa acacgcattg tccgccaatc gattcaaaac cgcgacggat
    22021 gccgccaatt ccggcaaata gatcgataaa gcgaaacgca taatgcggat gatgtgccga
    22081 tggtttgggt aacaacgttt gcagatgggc gaactctttc tcacttaaac ggtgccatgc
    22141 cgagtcattc gccagcgcac gttttaaaat tgccgcgctc cagtgattct cacccacacc
    22201 attaagctgc gccaccagcg ttttaacatc ataaatttgc agcagtttct ccagcattgc
    22261 ctgtgcggca ttcccggtgc tgtatgaatc ggttactgat atattttcct gcatagattt
    22321 aaccggccat ctgagatggc cgacaggtta acataattac agccatttag ggccacgcgc
    22381 ttatcccgcg ggtgaaaacg catctatcac cttgtgatcg acaccttcat tctcccccgc
    22441 cagttcggca ctgagcttcg ccataaactc gactaaaaaa tgcgcgttgt gctgcgccag
    22501 ctgcttgcct cttgcggttt gcatggtttg cggtagcttc agcaacttag tctggaaatg
    22561 atccagcgcg tagcgtttat catcaagcgg gcgatgctgt gcgaacggat cttcaccatc
    22621 aaacagcgcc acgcccaatg cccctgaaac ggcaaacacg cgcgccaggc caatcgcccc
    22681 cagggcttcc agccgatccg catcctggac gattttagct tccgttgtta agggggcgat
    22741 ttgcgcgctg aaactgtgag cggcaatggc atgacaaacg gcctcgattt tctccgccgg
    22801 aaattgctcg aactcttcac ggagcaggcg acgcgtctct tctgccgcca ggattgaaga
    22861 acgctgcctt tgcggatgat ttttcgccag gctcacaata tcgtgaaaat aacaggcggt
    22921 taaaatcacc agcatatcga catcgtcatc tgccgccagc ttctgtgccg cgtcctggtg
    22981 ctgatgatgg tttttcaacc agttttcgaa ctgtgcctgc cagtgttgta agtccatacg
    23041 cgctccgttg ccaaagagtc gccaccttag cagtttttat tcatagcgtc tgctacgtgc
    23101 gggacgaggt cgggtgtacc aggcaatgct gcctaacaat gccccgacca gagccataaa
    23161 taaaaatccg accagtgccc ctaaccactt tcccgcggta gagcctaaat cgctttcaaa
    23221 tcccgacacc ggggcatcag ggatccggct catcacccag atgtaacgcg ccattgccca
    23281 gacgaaatag ccacaccatg tatagaaagc ccacaaagcc aatttgccgc cagggctgcg
    23341 agagagtctc tcttccatct ttgtcgaacc gtattccatt ttgagataac ccggaaaatt
    23401 cagacttagt actattatgt gataaaagtc acatttttcc acattggatg agccagtcaa
    23461 gactcaatca ttttttgaac aaatagataa attttgcgca acggcaataa ttactggaaa
    23521 aacagcaata aagatttatt tatatccttg gtaatagttt tattaattca cccaaacaat
    23581 atattagcac tgaatacaat tatacctttt ttgatttctg tgtcatgata accccgttat
    23641 tagcctttta tcgtcttgtt tatatttttt gggccggcat gatgccggct ttttttatac
    23701 cttcattaat gtgcgcgtga tcacgcctgc cg
//
LOCUS       DAEEKQ010000053        23235 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.053, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000053 DAEEKQ010000000
VERSION     DAEEKQ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23235
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.053"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(38..238)
                     /gene="glgS"
                     /locus_tag="KLK57_001986"
     CDS             complement(38..238)
                     /gene="glgS"
                     /locus_tag="KLK57_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HBE0892355.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            507..1136
                     /locus_tag="KLK57_001987"
     CDS             507..1136
                     /locus_tag="KLK57_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HBE0892356.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYNSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKIFTKGVKVLIICRLSATRYLAENNPWPQIL"
     gene            1163..2824
                     /locus_tag="KLK57_001988"
     CDS             1163..2824
                     /locus_tag="KLK57_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBE0892357.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            3063..3122
                     /locus_tag="KLK57_001989"
     CDS             3063..3122
                     /locus_tag="KLK57_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HBE0892358.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
     gene            3439..3498
                     /gene="ibsE"
                     /locus_tag="KLK57_001990"
     CDS             3439..3498
                     /gene="ibsE"
                     /locus_tag="KLK57_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HBE0892359.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="KLK57_001991"
     CDS             complement(3618..5051)
                     /gene="hldE"
                     /locus_tag="KLK57_001991"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HBE0892360.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPSSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKQGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="KLK57_001992"
     CDS             complement(5099..7939)
                     /gene="glnE"
                     /locus_tag="KLK57_001992"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HBE0892361.1"
                     /translation="MKPLSSPLQQYRQTVVERLPEPLAEEPLSVQAKSVLTFSDFVQD
                     SIIAHREWLTELESQPPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSCSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMNNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLISDFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALNHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSTEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGRTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSGDCFTAERDLVRASWQKWLVEE"
     gene            complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="KLK57_001993"
     CDS             complement(7962..9263)
                     /gene="ygiF"
                     /locus_tag="KLK57_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HBE0892362.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQFPSEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSHHAAELKSVFCQPLGDLYRDQLPRLTRDIDSILLLAGYYDPVVAH
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            9505..10125
                     /locus_tag="KLK57_001994"
     CDS             9505..10125
                     /locus_tag="KLK57_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HBE0892363.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            10189..11427
                     /gene="cca"
                     /locus_tag="KLK57_001995"
     CDS             10189..11427
                     /gene="cca"
                     /locus_tag="KLK57_001995"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HBE0892364.1"
                     /translation="MKIYLVGGAIRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTDFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="KLK57_001996"
     CDS             complement(11590..12411)
                     /gene="bacA"
                     /locus_tag="KLK57_001996"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HBE0892365.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDISMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(12502..12870)
                     /gene="folB"
                     /locus_tag="KLK57_001997"
     CDS             complement(12502..12870)
                     /gene="folB"
                     /locus_tag="KLK57_001997"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HBE0892366.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAM
                     ARAANVGVIIERGNNLKENN"
     gene            12975..13592
                     /gene="plsY"
                     /locus_tag="KLK57_001998"
     CDS             12975..13592
                     /gene="plsY"
                     /locus_tag="KLK57_001998"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HBE0892367.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="KLK57_001999"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(13605..14537)
                     /gene="ttdR"
                     /locus_tag="KLK57_001999"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            14744..15655
                     /gene="ttdA"
                     /locus_tag="KLK57_002000"
     CDS             14744..15655
                     /gene="ttdA"
                     /locus_tag="KLK57_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HBE0892368.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEDVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            15652..16257
                     /gene="ttdB"
                     /locus_tag="KLK57_002001"
     CDS             15652..16257
                     /gene="ttdB"
                     /locus_tag="KLK57_002001"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HBE0892369.1"
                     /translation="MKKILTTPIKAEDLQDIRVDDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            16306..17769
                     /gene="ttdT"
                     /locus_tag="KLK57_002002"
     CDS             16306..17769
                     /gene="ttdT"
                     /locus_tag="KLK57_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HBE0892370.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLIVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="KLK57_002003"
     CDS             complement(17812..18825)
                     /gene="tsaD"
                     /locus_tag="KLK57_002003"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HBE0892371.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            19062..19277
                     /gene="rpsU"
                     /locus_tag="KLK57_002004"
     CDS             19062..19277
                     /gene="rpsU"
                     /locus_tag="KLK57_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HBE0892372.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            19388..21133
                     /gene="dnaG"
                     /locus_tag="KLK57_002005"
     CDS             19388..21133
                     /gene="dnaG"
                     /locus_tag="KLK57_002005"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBE0892373.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            21328..23169
                     /gene="rpoD"
                     /locus_tag="KLK57_002006"
     CDS             21328..23169
                     /gene="rpoD"
                     /locus_tag="KLK57_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HBE0892374.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT         5655 a         6331 c         5904 g         5345 t
ORIGIN      
        1 aatacaaaca ctaaaacggg ccatcaggcc cgttatatca gtgctctaac tccagctctc
       61 ttgcctgcat catctgttgg caataccagg cataacgtgc gcaaaaccag gttctatgtt
      121 cttcatccat gttaccagta acggcaagaa tatcgaccgg gcgaccttct gcgtgcattc
      181 tggcaaaact acgcgccagg aaatcgaaat tatttaaaga attaagacta tgatccataa
      241 gcacatcctc cggttatcgc atacctttta aggccatatg ctttttctga tttcagtatt
      301 ccccaatcag tgtagtgcct ttaaacataa cgtgcgtaaa tatattgtcc ccgatcacac
      361 tttttagtga aaaataaaaa actatattaa aaaactttct cttccagaac ttctctaaca
      421 tgtgttatta ttaacaaaga taaatgtaag caaggaaact atcccaaagt aaatattgcg
      481 tcactaaatg gacattggag tgacatatga ttttattcgc cgactataat accccttatc
      541 ttttcgctat ttcctttgta ctgttgatcg gccttttaga aattttagcg cttatctgcg
      601 gtcacatgct ctccggtgct cttgatgcac atcttgacca ttataattcc ataaccacag
      661 gtcatattag ccaggcactt cattatctca atatcggaag attgccagct ctcgtcgttc
      721 tctgcttgct ggctggtttt tttggtctta ttggcatcct gttgcagcat gcctgcatca
      781 tggtctggca atcaccactg tcgaacctgt tcgtggttcc tgtcagttta ctgtttacga
      841 ttattgcggt gcattatacc ggtaaaatag ttgcgccctg gattccacgc gaccatagtt
      901 cagctattac agaagaagaa tatattggta gcatggcatt aattaccggt catcaagcca
      961 cttcaggtaa tccctgcgaa ggaaaactca ccgatcaatt tggccaaata cattatttac
     1021 tactggagcc tgaagaagga aaaattttca ctaaaggagt caaagtgtta attatttgcc
     1081 gactttctgc aacgcgatat ttggcggaaa acaatccctg gccacaaata ttgtaattta
     1141 ttaatcaaaa ggaaatttaa taatggatga tattgttaat tctgtgccct cctggatgtt
     1201 taccgcgatt attgccgtat gcattctgtt tattattgga attattttcg ccaggctcta
     1261 tcgtcgcgct tcggcagagc aagcttttgt tcgtactggt ttaggtgggc aaaaagtggt
     1321 gatgagcggt ggcgcaatcg tgatgccgat ctttcatgaa ataatcccca tcaatatgaa
     1381 tactctgaag ctggaagtca gccgctcaac cattgatagc ctgattacga aagatcgtat
     1441 gcgtgtcgat gtagtagtcg ctttctttgt acgggtaaaa ccgtctgtag aaggcatcgc
     1501 cactgcggcg cagacgttgg ggcaacgcac gctatcacca gaagacttac gtatgttggt
     1561 tgaagataaa tttgtcgatg ccctccgtgc aacagctgca caaatgacca tgcatgagtt
     1621 acaggatacc cgcgagaact tcgtgcaggg agtacaaaat actgtcgcag aagacctatc
     1681 gaaaaacggt cttgagctgg aaagcgtttc acttaccaac tttaaccaga cctcgaaaga
     1741 acatttcaat ccgaacaatg cctttgacgc cgaaggttta accaaactga ctcaggagac
     1801 ggagcgccgt cgtcgcgaac gtaacgaagt tgaacaggat gtagaagttg cggtgcgtga
     1861 gaaaaaccgt gatgcacttt cgcgcaagtt ggagattgaa caacaagaag cgtttatgac
     1921 gcttgagcag gagcagcagg ttaaaacccg tactgccgaa cagaatgcac gtattgcggc
     1981 ttttgaagct gaacgtcgtc gtgaagcaga gcagacgcga attctggctg aacgacaaat
     2041 tcaggaaacg gagatcgagc gcgagcaggc cgtccgctca agaaaggttg aggctgaacg
     2101 tgaagttcgt attaaagaga tcgaacagca gcaggtcacc gaaatcgcca accagacgaa
     2161 atcgatcgct attgccgcca aatcggaaca acagtcccag gcagaagcgc gtgctaacct
     2221 tgcactcgca gaagcagtaa gtgcgcaaca aaacgtagaa accactcgcc agaccgcaga
     2281 agccgatcgt gctaaacaag ttgccctaat cgctgccgcg caggatgcag aaaccaaagc
     2341 ggttgaactg accgtgcggg cgaaagcaga aaaagaagcc gcagagatgc aggcggcggc
     2401 tatcgttgag ttagccgaag ctacacgtaa aaagggtctg gcggaagcag aagcacaacg
     2461 tgcgctgaac gatgctatca acgtactttc tgatgaacaa accagcctta aattcaaact
     2521 ggccttgttg caggcgctac ctgcggtaat agagaaatcc gttgagccga tgaagtccat
     2581 tgacggtatc aagattattc aggtcgatgg tctgaatcgt ggcagcgctg cgggtgatgc
     2641 aaacacgggt aatgtggggg gcggaaacct agcggaacaa gcattatcag ccgctctctc
     2701 ttaccgcaca caggcaccgc tgattgactc cttgctcaat gaaattggcg tttcaggcgg
     2761 ctcactggcg gcattgactt cacccttaac ctcaacaact cccgtcgccg aaaacgtaga
     2821 ataaattttc agctatgaac gtccctggca caaaaagccg gggatttttt atatctgcgt
     2881 tccgctaaaa actgcaaatg atcaggccgt tgcaacgttt tgcgtgaccg ctcggggaag
     2941 gcaaaactgc ctctgggaaa gcgttgcgcg aggtccggcg ctcatcaaca atcggggagt
     3001 cagcaaaggg ctgaaacggg aaagcccctc ccgaggaagg ggccttacag aaggaaaggg
     3061 ttatgatgaa gctcgtcatc atactgattg tgttgttact cgtaagtttc gcagcttatt
     3121 aacagccaat cagagggggg agaaatcctc cctcaccctt gttcctttac tttaggttga
     3181 aaaaaaatca gcgtcaagaa ggtttcatta acacatatcc ctggcccaaa aagccgggga
     3241 ttttttatat ctgcgttccg ctaaaaggtg caaatgctca ggacgttgca gcgttttgcg
     3301 tgatcgctcg gggaaggcaa aactgcttca gggaaagcgt tgcgcggggt ccggcgctca
     3361 tcaacaatcg gggagtcagc aaggggctga aacgggaaag cccctcccga ggaaggggcc
     3421 ttatagaagg aaagggttat gatgaagttc gtcatcatac tggttgtgtt gttactgtta
     3481 agtttcccga cttactaaca actgatcaga gggggaagaa atcctccctt acccttgttc
     3541 ctttacttta ggttgaaaaa acaacagcgt caataggcct gcctgctaca aagcgagatc
     3601 tgtgaaccgc tttccggtta gcctttttta tcctgttgga tcttcttgat gatgttagtg
     3661 gtcgagcaac cgtcttcgaa gttgagcacc agcacttcgc cgccgttggc ccagacttct
     3721 ttactcccgg cgatctcttc tggtttatag tcgccgccct tcaccagtaa atctggcagg
     3781 atcccggcga tcaagcgctg cggcgtgtct tcttcaaacg acaccaccca gtcgaccgct
     3841 tccagtgcgc ccagcacaat catacgctgt tcgagcgggt taacggggcg ggaatcccct
     3901 ttcagccgtt tggtggaggc atcgctgttc acagcgacaa tcagacggtc accctgtttg
     3961 cgagcatttg ccagatagga gacgtgcccg gcgtgcagga tgtcaaagac accgttggtc
     4021 attaccacct tttcgccgcg tttacgcgct gccgctacgg ccagcttcag ttcctcttcg
     4081 gtcatcacgc caaagccggt atctgcgcgc ccacgcactg cattttccag ctcgatcggc
     4141 gaaaccgtgg aggttcccag tttgccgacc accacgccag ccgccgcatt ggcaaagaag
     4201 caggcttctt ccagcgaatt acccgctgcc agcgttgccg ccaggacgcc aatcaccgtg
     4261 tcgcccgcac cggtaacgtc atacacttcc tgcgcctggg ttggcatatg cagcggcgct
     4321 ttacccggtt gcagcagcga cataccctgt tcggaacggg tcactaacag agccgagagt
     4381 tcgtaatcgg caatcagttt catgccgcgc tcaacaatct cttcttcggt cttacattta
     4441 ccgacaacag cttcaaattc cgagagattc ggcgttaaca gcgtagcgcc gcggtagcgc
     4501 tcaaaatcgg taccttttgg atcaatcagc accggaacgc ccgctttacg cgccagttgg
     4561 atcatctgct gtacgcttgc cagcgcacct ttggcgtagt cagaaagcac cagcgcgcca
     4621 atcgaactca gcgcctgatt aatccgctcg tgcagcggct gcggatcaac gccttcgaaa
     4681 ccttcttcga aatccagacg gatcagctgt tggttgcggg aaagtacccg taatttggtg
     4741 atggtcggat gcgtcggtac agaaacgaag tcgcatttga cgttgacgtc ggccagagat
     4801 ttactcagcg cgcgcgctgc atcgtcaatg cccgtcaacc cgaccaggcg tgcattagca
     4861 ccgagagaag cgatattcat cgccacgtta gccgcgccgc ccggacgttc ttcgatggta
     4921 ttcactttaa ccacgggcac cggcgcttcc ggcgagatac ggctggaggg accgtaccag
     4981 taacgatcca gcattacatc accaaccacc atcactcctg cacgttcaaa ctctggcagc
     5041 gttactttca ttcctgtctc ctgagagatt caaaatttgc gcgcgataat accatacttc
     5101 attcttccac cagccacttc tgccagcttg cccgcaccag atcacgctct gcggtgaagc
     5161 aatcccccga cacatggccc ggcaattcct gtaatgccag atgatgaagt tcatcgcgca
     5221 gcgtcgtgta agcacgggtc agcgccatcg cttcctgctc ttccataatg tcgttttgcg
     5281 ccagcagctc cagaatacgc acgttgtctg accagcgcgt taacttcggt ttttcatgag
     5341 cgtagcgcaa caccagatat tgggtaataa attcgatatc ggtaattccg ccttcatcag
     5401 ctttgatatc aaagcgatcg cgatgtttat tgccgagatg agcgcgcatt ttctcgcgca
     5461 tttcccgcac ttccgtttgc agagttctac cttcacgcgg cagcgtcata atctcgcgac
     5521 gcactgcgtc aaagtgcgcg gtgagctgcg gatcgccgta cactacccgc gcacgcacca
     5581 gcgcctgatg ttcccacgtc caggcctcgt ttttctgata atcggcaaat gcttctgtgg
     5641 aggtaaccag cattcccgcc gccccggacg ggcgcagccg cgcatccact tcgtacaaaa
     5701 tgccggaaga ggttcgcgtg ctgaacaggt gcataatgcg ttgcgccaga cgaagataaa
     5761 actgtcgtcc gtcgatttcc cgctcgccgt cagtcatcgc atccattggg caatcatgga
     5821 ggaagatcag gtcaagatcg gaactgtagc ctaactccca gccgcccagc ttgccgtagc
     5881 cgaccactgc aaaaccacgc ccttcgcgat cgttgaggtg attcggctta ccgtagcggg
     5941 caaccatttg aacccacgcc tgctgaacga cggcatctat catggcttcc gccagccagg
     6001 ttaagtgatc gctcactttc atcaccggta gcgtaccagc gatatccgct gcggcgatgc
     6061 gtaacagctg cgcctgtttg aactgacgca gcgcctcaag ctgttgctct tcgtcatctt
     6121 ccggcacgcg cagcaaatac tggcgcaact catcgcggta ggcatcggtc gccgtcggct
     6181 ggtaaagagt gtttggatcg agcaattcat ccagcaataa tggataacgc gccagctggc
     6241 tggcaatcat cggcgatgcg gcacacagag aaatcaaatg gttaagcgcc gcagggaatt
     6301 cactgagcaa ttctaaatag gtggtgcggg taacaatccc caccagcaag gcggtaatgc
     6361 gcgacagcgt gacagcagcg tcttcacgcg cacagacatc acttagcaga tgcggcatca
     6421 gatggtcgag cacctgacgt cctcgcggcc cgatggtgcg cttatccagc tctttgcgga
     6481 aatcggaaat cagcgttagc acctgtttgc gatcatcctc gctaagatgc gccagcactg
     6541 gcgtagtgtc atcttcctgc aacgcatcct gccacagctc acgccactgt tccgacagcg
     6601 actcttcctg agtttcactt tcgtcgtcgc caatcaattc attaaacacc cggcgcacat
     6661 tgttcatatg tgcggtcagc gccccggtca gttgcggcca gtcagcaaag tccatcgccc
     6721 acgccagccg cgcacggtta agctcatcag aaggaagcgt ctgggtttgt tcgtcgttaa
     6781 tgctttgcag caggttttcc agacgccgca ggaacagata cgccactcgc aattgttcag
     6841 catcgttttc agaaagcaga tgcagcgcag caatggcgct gagcgttggc agtaaagagc
     6901 acgattgcag cgacggttcg cgcccgccgc gaatgagctg gaatacctgg acgataaatt
     6961 caatttcgcg aataccaccc gcgccaagtt tgatgttgtc ggtcaaaccg cgccgacgca
     7021 cttcacgggc aatcattcct ttcatgttgc gcagcgactg aatcacgctg aaatcgatgt
     7081 aacgacggaa aacaaacggg cgtagcatcg cacgcaactc gttagcatag acgccttcgc
     7141 tatcgcccat aatccgcgct ttgaccatcg cgtaacgctc ccagtcgcgc ccctgctcct
     7201 ggtaataatc ctccaacgcg gcaaagctca gcaccagcgg gccactttcg ccaaacggac
     7261 gcagtcgcat atccacgcga tagacgaagc catcctgcgt tggttgatcc agcactttaa
     7321 tcagccgctg ccccatgcgg gtaaaaaact gcgcgttatc cagttcccgg cgtccgccct
     7381 gcgtacaacc atgttccggc caggcaaaaa tcagatcgat atcagaggag aaattcagtt
     7441 ccccgccgcc cagcttaccc atgcctaaga tcagcagcgg ttgcgcttcg ccctgcgcat
     7501 tgcacggcgt tccccactcg cggcagcagg cgtcatacag ccagtcacgc gccgcaacaa
     7561 tcagcgtctc cgccagataa ctgagctgct gcaatatgct ctcttcagta accagtgcca
     7621 gcgtttgcgc ccatgcgatg cgcaccataa tgcgccgccg gaatagccgc agctcgcgca
     7681 ttaacccggc ttcgtcactc acattactga gcgcctcctg caaccatgca gcgtaatgct
     7741 gccattcatc ggcctgcggc ggttggcttt ccagttccgt caaccactct cgatgcgcaa
     7801 ttatgctgtc ctgcacaaaa tcactgaaag taagtactga cttcgcctgt acacttagtg
     7861 gttcctcggc taaaggctct ggcagccgct caacaacggt ctgcctgtac tgctgtaacg
     7921 gtgaagagag cggcttcata agcgatttta tccttgcctg attaacgttt tccgctgtgc
     7981 aaccagaacg gttcctgatt gtttgcctca ttacggaaat gctcaatttc aatgcgttgc
     8041 ccggtcgcaa tagcgtgacg cagcccctgc cagttctcca gccaggcatg cgcgacgaca
     8101 ggatcatagt aacccgccag caacagtatt gagtcaatgt cacgcgtcag gcgtggcaac
     8161 tggtcacggt agagatcgcc taacggctgg cagaaaacgc ttttcagttc agcggcatgg
     8221 tgggaaagat ggatatcggc aaagcgtttg aaggagtcgc tgattttgcc ctgcgctttg
     8281 gcatctaaaa atggctgcca tgctttgctt accaaccatt cggtcaacgc cagcttcgcc
     8341 attgccgttt cggtagagta gaccgccgtc acggcagaaa ccgcagaagc aatggtcgct
     8401 tcgcattggg tcagcaaatc acgtaagtga gtgctcgctt tacgcggcac aataccaccg
     8461 aacagcatca gcgtatgacg gaccaggcta atggctgcca gcacctgttc tttcgccgca
     8521 tcgttaccgc gtacccacag ttcttcatga tactgccatt gcgctaacgc cagctcgagc
     8581 gccgcttcca gcccctgttc tacatcggct tttgccgcaa catgcaaaat ggtggtcggt
     8641 ttgatttcac gcgccggatt gccctgcgcc agatgatagc cacgcgccgc tttgctcagg
     8701 ctgccctggc gtaatccggt ttgcgatacc agttggttcg ccagtttcag caccgcgcgc
     8761 gtgtcgccgc taagcagttc cagttccagc tcacagatag gttcagcaaa ttcccccgct
     8821 ttcacctccc cctgatcgag agcgatttca attcggctgc catcgaccgc caccagccat
     8881 ttttcgcgat aaaaatcggt gctgaacagc ggttgtacgc gggaagcgag atcggcaggc
     8941 aattcaccgt tcggccagac ttccgaggga aactgcgcca ggtcgagcgt cggttcgctc
     9001 aacgccacgt tatattccgg gcgctggtgt aagccgcctg tcactcttcc tgcaactttc
     9061 atggtcatct catagcgacc gttttcgcca cgaatacgta agcccatatc gtgcctacgc
     9121 agccagttat ccggcgtttc gtagtaaata ttcagcaact gcacggggtc atggtgctcg
     9181 ccgcccagcg tattgagatg gtcacgcaac gcctcaacgg cactgtgatt aacaataaac
     9241 tttaattcga tttcctgagc catggccttt acttatcggt tatgtcacat aagtgacgat
     9301 gaacggcgaa cttaatgcga tcttttttgt cagtagatag tattttgcgc caaattgcca
     9361 tgcaacgggc aatttgacgg gcgtaaaagt ttgaagcagt ggcaagacct gacacagagg
     9421 attccgattg atgtcgaatg ctttgcgtag cgacactgat gccactacta tcgttccact
     9481 ttccatgaca ataacgacag cctgatgcca aaattacgcc tgatcggatt aactttactc
     9541 gcacttagcg cgactgccgt ctcacacgct gaagaaacgc gctatgtttc tgacgaactg
     9601 aatacctggg tccgtagcgg tccgggagat cattatcgcc tcgtgggcac ggttaacgcc
     9661 ggcgaggaag tgaccttatt acaaactgac gccaacacca attatgccca ggtgaaagac
     9721 agctctggcc gtaccgcctg gatcccgttg aaacaactta gcactgagcc aagcctgcgc
     9781 tcccgtgtgc cagatctgga aaatcaggtc aaaaccctga ccgataaact caccaatatc
     9841 gataacacct ggaatcagcg caccgcagaa atgcagcaaa aagtggcgca gagcgacagc
     9901 gtgatcaacg ggttaaaaga agaaaatcag aaactgaaaa acgagctgat tgtcgcgcag
     9961 aaaaaggtcg atgccgccag cgtacagctg gatgacaaac agcgcaccat catcatgcaa
    10021 tggtttatgt atggcggtgg cgtgctgggg cttggcttgc tgctcggtct ggtactgccg
    10081 cacctgatcc caagccgcaa acgcaaagat cgctggatga actaaatcgc cttcttcgtt
    10141 gcactgtctt attattagga attgtgtatt tgacgaaggg atgatggcgt gaagatttat
    10201 ctggtcggtg gtgctattcg ggatgcattg ttagggctac cggtcaaaga cagagattgg
    10261 gtggtagtcg gcagtacgcc acaggagatg ctcgacgcgg gctaccagca ggtaggccgc
    10321 gattttcctg tgtttctaca tccgcaaacg catgaagagt atgcgctggc acgtaccgaa
    10381 cggaaatccg gttccggtta caccgatttt acttgctatg ccgcaccgga tgtcacgctg
    10441 gaagatgatc ttaagcgtcg cgatctgacc attaatgcgc tggcccagga cgataacggc
    10501 gagattatcg acccgtacaa cggtctgggc gatctgcaaa atcgtctgtt gcgccatgtt
    10561 tcccccgctt ttggcgaaga tccgttacgc gtactgcgcg tggcgcgttt tgctgcgcgt
    10621 tatgcccacc tcggttttcg tattgccgat gaaactctga cgctgatgcg cgagatgacc
    10681 catgcgggtg aactggaaca cctgacgcct gaacgggtat ggaaagagac ggaaagcgcc
    10741 ctcaccaccc gcaatccaca ggtgttcttc caggtactgc gcgattgcgg cgcactgcgc
    10801 gttttattcc cggaaattga cgcactgttt ggcgttccgg cccctgccag gtggcatccg
    10861 gaaatcgaca cgggtattca taccttaatg acgctctcaa tggcggcgat gctgagtccg
    10921 caggtcgatg tccgtttcgc aactttatgc cacgatctcg gtaaaggact gacgccgcca
    10981 gaactttggc cgcgtcatca tggtcacggt ccggcgggtg tgaaattagt ggaacaactc
    11041 tgccagcgtc tgcgcgtgcc aaatgaaatt cgcgatttag ccagactggt ggctgagttt
    11101 cacgatctca tccacacctt cccaatgctg aacccaaaaa ctatcgtcaa attgtttgat
    11161 tccatcgacg cctggcgtaa accgcagcgt gtcgagcaac tggcgctgac cagcgaggct
    11221 gacgtacgcg gcagaaccgg ttttgaatca gcggactacc cgcaaggccg ttggttgcgc
    11281 gaagcctggg aagtggcgca gtcagtgccg acaaaagcgg tcgttgaagc gggatttaaa
    11341 ggtgtggaga ttcgcgagga gctgacccga cggcggattg cggcggtagc cagttggaag
    11401 gaacaacgtt gcccaaagcc tgactgagta taaaactaaa cgttaacacg aaccatcccc
    11461 tcgccccttc ggggagaggg ttagggtgag gggaacagac cagcaccggt gcgaacaatc
    11521 acccccaccc cggccctctc cctacagagc gagggagaaa accaggcctg ctcccgaacc
    11581 ttttctcaat caaaagaaca cgacgtacac cgcagccgcc acaataaagc gataaatggc
    11641 gaacgggata aacgaaatgc gcttaatcaa ttgcaggaag gttttaatcg ctatcagcgc
    11701 caccacaaaa gcggtgataa acccaacggc aaacatcgag atatcgccgg ttgtcaggaa
    11761 gccccagctt ttgtagagat cgagcgccgt tgcgcccatc atcatcggta ccgccagcag
    11821 gaacgagaac tcggaagcag cgtaacggct cacgcccatc agcatcccac ctgaaatggt
    11881 ggccccggaa cgggagaaac ccggccacag cgccagacac tggaaacagc caatcataaa
    11941 cgcctgacga taggtcatat catcaagacc cggcgcacgc ggctctttcg gcttcaggca
    12001 ttcggcggca atcagcaaca aaccgccaac gaccagtgca tacatcacat ttatcgggtt
    12061 aaacaatgac ttaatcgtgt cgtggaacaa cagccccagt accaccgccg gaatcatccc
    12121 cagcaaaatg tggatcagcg ttaaacgacc tttgctttct ccttcgtgct gcaacgggcg
    12181 gccaaagtgg atgccaatca ggccaaacag acgccgccag aacatcacta ctaccgccag
    12241 aattgatcct aactggatca caacttcaaa ggttttcgcc gtgtcgccct cgaaccccaa
    12301 caagtgaccg acaataatca tatggcccgt gctggatacc ggcagaaatt ctgtcaatcc
    12361 ttcgaccaca cccaatattg ccgctatcag cagcgagtgc atatcgctca tcaataaacc
    12421 cctaaattat taaaatgtac cgcttgtccg gactactgca gtaagaccag gttataaccg
    12481 tttggtttaa cagctgtaaa attaattatt ttctttcaga ttattgccac gctcaatgat
    12541 tacaccaaca ttcgccgccc gcgccattgc gcctggcttg ctgagtttga tgcgcaccca
    12601 cggcgagttg aagcgtgcta acagcagctc cgccacctct tcagccacgc gttccaccag
    12661 cgcaaaacgc gccccctcga cgtggctgac caccgtttct gcaatgtcag cgtaactgag
    12721 gcaatccgcc acatcatcac ttttcgccgc tttacggtta tcccatgcca tttcgatatc
    12781 gaacactaac ttctgttcga tggtctgttc ccagtcgtaa acaccaatag tggtgattac
    12841 cgaaagttgc tctataaata caatatccat cacgtcctgc ctgctttttg gctaaccgga
    12901 taccacttcc ggcgaaatgt gcgtattatc cacagattca tcgttgaaca cgaattttca
    12961 aaacggaaca gcttatgagt gcaatcgcgc ctggaatgat cctcatcgcg tacctctgcg
    13021 gctccatttc cagtgccatt ctggtttgcc gcttgtgtgg gctgcccgat ccgcgaacca
    13081 gcggctccgg caatccaggc gcaaccaatg tgttacgtat cggtggcaag ggagcagccg
    13141 tagcagtact gattttcgac gttctgaaag gaatgttgcc cgtctggggc gcgtatgaat
    13201 taggtgtcag ccccttctgg ttaggcttaa ttgccatcgc cgcctgtctt ggacacatct
    13261 ggcccgtttt cttcggattt aaaggaggaa aaggcgttgc taccgctttt ggtgccatcg
    13321 cacccattgg ctgggatctc accggagtaa tggcgggaac ctggttactg accgtgctat
    13381 tgagcggata ctcgtcgctg ggagcgattg tcagtgcact gattgctccg ttttatgtct
    13441 ggtggtttaa gccacaattc accttcccgg tttcgatgct ctcttgcctg atcctgctgc
    13501 gtcatcatga caacatccag cgtctgtggc gtcgtcagga gacaaaaatc tggacgaaat
    13561 tcaaaagaaa gcgcgaaaag gatcccgagt gatttctggt ggatctacat gacctgatag
    13621 ccttcatcgg gcttgcccag ccgttgctgg caccatgccg ccagaaattc cacgcagaca
    13681 cgtaatttca tgctgcgata gagcggctcc cggtaaacag cccagatatt ggcgctttgt
    13741 gcatactccg gcaatacttg caccagtttg ccactctcca gaaacggcaa cacatcccac
    13801 tcggaacgca gcataatccc tttgccctcc agcgcccatt gcaacactat ctcgccgcta
    13861 ttggaggaaa gatgcccgct aaccttcacc gattttttct cctgaccgtt ccccaactcc
    13921 catattccgt gggtcatatc gcgctctttg gtcaccagac agtcatgacg acttaattcc
    13981 tgtaatgatt gtggctgcgg atatttttgc agatactcag gtgctgcgca taatattctt
    14041 ttatttttcg ttaacagatg cgcaatataa taatcaggaa tttcgtcatt aatacgaata
    14101 tccagatcaa tattatcctg cactaaatca atttgccgat cgaacaattc aaaatgcacc
    14161 tgtaactcag gataattgcg cataagttcg gtaatggctg gcgcaatatg gctgcgccca
    14221 aaaccaaaac tgcaaccaat acgaatcatc ccctccgggc gcgttttgat ttacgtgacg
    14281 tcatcgacca gtcgctgata ttgggtgagg atttccagcg catgttcgta acaacgttgg
    14341 ccactttcag tcagcgccac gccgcgagcg gagcggttca gcagcgttgt tgccagcgta
    14401 ttttcaagga tttggatccg cttggtgaca aatgcaggcg tctgcccaag cgtcgccgct
    14461 gcggcgctaa aactcccgct gtggacgatt tctaccagta cctgcaagtc tttggctaaa
    14521 ggccagctat tcagcatcgt taattatccg cagttgtgat aagcgcagtg tatttcgcaa
    14581 aacattgcca ttccctgctc tggcgcaata ttcattctta ttttcgcgtt gattcacgaa
    14641 ttgttaatta catattcacg gtggcaaaaa atataaaacc acatttttag tggtagtttg
    14701 tggcggtgaa tttttccaga caaatacaaa aactggagtt gccatgatga gcgaaagtaa
    14761 taagcaacag gcagtgaata agttgacgga gattgtcgct aactttaccg ccatgatttc
    14821 tacccgaatg cctgatgacg tggtggataa actaaaacag ctaaaggatg ccgaaacgtc
    14881 gtcgatgggg aaaattatct accatacgat gttcgacaac atgcaaaaag cgattgacct
    14941 gaatcgtcct gcctgtcagg acaccgggga gattatgttc ttcgttaaag tcggttcccg
    15001 cttcccactg cttggcgagc tgcaaagcat actcaaacaa gccgtggaag aggcaaccgt
    15061 caaagcgcca ctacgtcaca atgcggtaga aatttttgac gaagtaaaca ccggcaaaaa
    15121 taccggtagc ggcgtaccgt gggtcacctg ggacatcatc cccgacaatg acgatgcgga
    15181 aatcgaagtt tacatggcag gcggcggctg cacgctacct ggccgctcga aagtgttaat
    15241 gccgtcagaa ggctacgaag acgtggtgaa attcgtcttc gaaaatatct ccaccctcgc
    15301 cgtaaacgcc tgtccaccgg tactggtggg cgtgggcatc gccacctcgg tggaaaccgc
    15361 cgccgtactc tcgcgtaaag ccattttgcg cccgattggc agccgccatc ccaatccaaa
    15421 agcggcagaa ctggagctac gcctggaaga aggactcaac cgtctgggga ttggtccaca
    15481 agggctgact ggcaacagtt cagtgatggg cgtacatatc gaatctgccg cccgccatcc
    15541 gtcaaccatc ggcgttgctg tctctaccgg ctgctgggcg catcgtcgcg gcacactgct
    15601 ggttcatgcc gatctcacct ttgaaaatct gtctcacacc cggagcgcgt tatgaaaaag
    15661 atcctgacaa ccccgatcaa agctgaagat ctgcaagata ttcgcgtcga cgatgtgatc
    15721 tacctgaccg gtacgctggt gacctgccgt gacgtttgtc accgccgctt gatcgaactg
    15781 aaacgtccga tcccttacga tctcaacggc aaagcgattt tccacgctgg ccccatcgtg
    15841 cgcaaaaacg gcgataaatg ggagatggtt tccgttggcc cgacaaccag tatgcgtatg
    15901 gaaagttttg aacgtgaatt tatcgagcag accggcgtga aactggtggt tggcaaaggc
    15961 ggaatggggc cgctgaccga agaaggctgc cagaaattca aggcgctaca tgtgattttc
    16021 ccggcaggct gcgcggtgct ggcggcaacc caggtggaag agattgaaga agtgcactgg
    16081 acagagctcg gaatgccgga gtcgctgtgg gtctgtcggg tcaaagagtt cggcccgctg
    16141 attgtctcta ttgataccca cggcaacaac ctgatagccg aaaacaaaaa gctgttcgcc
    16201 gaacgccgcg atcccatcgt ggaagagatc tgcgagcacg tccattacat caaataaccc
    16261 tcccggagag gctcgccccc tctccttttt cgcaggcata acacgatgaa accttccact
    16321 gaatggtggc gataccttgc gccgctggcg gtcatcgcca ttattgctct aattccggtt
    16381 cccgcagggc tggagagtca tacctggctc tactttgccg tttttactgg cgtgatcgtt
    16441 ggactgatcc tcgaacccgt gccgggtgcc gtggtggcga tggtgggtat atccatcatc
    16501 gccatactct ctccctggct gctgttcagc ccggagcagc tcgctcagcc aggctttaaa
    16561 ttcactgcaa aatccctctc gtgggccgtt tccggttttt ctaattcggt tatctggctg
    16621 attttcgccg cctttatgtt tggcacaggc tatgaaaaaa ccgggcttgg acgccgcatt
    16681 gcgctgattc tggtgaaaaa gatgggacat cgcacgctgt ttctcggcta tgcggtgatg
    16741 ttctccgagc tgatcctcgc acctgtaaca ccgtccaact cggcgcgtgg tgcggggatt
    16801 atctatccca tcatccgtaa cctgccaccg ctctatcaat cacaaccaaa cgacagcagt
    16861 tcgcgcagca ttggctcgta catcatgtgg atggggattg ttgccgactg cgtgaccagc
    16921 gccattttct tgacggcgat ggcaccaaac ttgctgttaa ttggactgat gaaaagcgca
    16981 tctcacgcca cgctgagttg gggcgactgg ttcctcggga tgttgccgct cagtattttg
    17041 ctggtgctgc tggttccctg gctggcttat gtgctgtacc cgccggtact gaagtctggc
    17101 gatcaggtgc cgcgctgggc agagacggaa ctgcaggcaa tgggcccgct ctgttcgcgt
    17161 gaaaaacgga tgctggggct gatagtaggc gcgctggtgc tgtggatttt cggcggtgat
    17221 tatatcgatg ccgcgatggt cggttacagc gtggtggcac tgatgctgct tctgcgcatt
    17281 atcagttggg acgacattgt cagtaataaa gcggcgtgga acgttttctt ctggctggcc
    17341 tcgcttatca ccctcgctac cggactcaac aacaccggtt ttattagctg gtttggcaaa
    17401 ctgttagcag gcagcttaag cggttattcg ccaacgatgg tgatggtggc gttgattgtg
    17461 gtgttttatc tgctgcgcta ctttttcgcc agcgccacgg cgtatacctc cgccctcgca
    17521 ccgatgatga ttgccgctgc gctggcgatg ccggaaatcc cgctgccggt gttctgcctg
    17581 atggttggcg cggcaattgg tctggggagc attcttacac catacgccac cggacccagt
    17641 ccgatttact acggtagtgg ttatctgcca acggcggatt actggcgact gggggcgatt
    17701 tttgggctga tattcctcgt gttgctggtg attaccggct tactgtggat gcccgtggtg
    17761 ttgctttaag tgaggacggt gccggaaatt gctctccggc acccaatagt tttacgcagc
    17821 cggtaactcc gccagcggcc agcgcggacg cacgctaacg ccgagatccg ccgtcgcgcc
    17881 tgctttaaac cgcaccattc cggcataggc gatcatcgcg ccgttatcag tacaaaactc
    17941 cggacgcgcg tagaacactt cgccgcggcg ttttttcatc atttcagcca gcttcgcccg
    18001 taacgtgcgg ttagcactca cgccgcccgc catgaccagt cgcttaaagc ccgtctgatc
    18061 caacgctcgc ttacacttaa tcatcaacgt atcgaccacc gcatcttcaa aggcgcgggc
    18121 gatatcagca cgcgtctggt cgtcggtgcc gttgtcacga atcgtgttcg ccgcaaaggt
    18181 tttcagacca gaaaagctga aatccagccc cggacggtcg gtcatcggac gcgggaaaac
    18241 aaagcgcccg gcagtaccct gagccgccat tttcgacagt aacggtccgc caggataatc
    18301 cagccccagc agcttcgcgg ttttatcaaa cgcttcaccg gcggcatcat cgatagactc
    18361 gccgagcagc tcgtactgac caatgccagt cacgctgatt aactgcgtat gaccgccgga
    18421 caccagcagc gcgacaaacg gaaattccgg cgggttatct tccagcatcg gcgctaacag
    18481 atgcccttcc atatggtgta ccgggattgc cggaacgttc caggcaaacg ccagagaacg
    18541 ccccacggtt gcgccaacca gcagtgcgcc gactaatcca gggcctgcgg tataggccac
    18601 agcatcaatg tcttttgccg ttaagccaga ctcctttagc gccgcctgga tcaacggtac
    18661 ggttttgcgc acgtgatcgc gggaggccag ttcaggcacg acgccgccat agtcagcgtg
    18721 caatttcacc tgactataca attggttggc taacaaacct ttttcatcgt cgtaaatggc
    18781 gatgccggtt tcatcgcagg aagtttcaat acccagtaca cgcatgactt gttttacctc
    18841 gctttattac cgcgcagtgt aggaccaatg cgggttgatg taaaactttg ttcgcccctg
    18901 gagaaagcct cgtgtatact cctcaccctt ataaaagtcc ctttcaaaaa ggccgcggtg
    18961 ctttacaaag cagcagcaat tgcagtaaaa ttccgcacca ttttgaaata agctggcgtt
    19021 gatgccagcg gcaaaccgaa ttaatcaaag gtgagaggca catgccggta attaaagtac
    19081 gtgaaaacga gccgttcgac gtagctctgc gtcgcttcaa gcgttcctgc gaaaaagcag
    19141 gtgttctggc ggaagttcgt cgtcgtgagt tctatgaaaa accgactacc gaacgtaagc
    19201 gcgctaaagc ttctgcagtg aaacgtcacg cgaagaaact ggctcgcgaa aacgcacgcc
    19261 gcactcgtct gtactaattc cccgagagcg ttgctctccg atcagaccga gttgtagttg
    19321 taaggccgtg cttccgaaag gaatgcgcgg cttattttcg tttatgaatt gctaaaaatc
    19381 ggggcctatg gctggacgaa tcccacgcgt attcattaat gatctgctgg cacgcactga
    19441 catcgtcgat ctgatcgatg cccgtgtgaa gctgaaaaag cagggcaaga atttccacgc
    19501 gtgttgtcca ttccacaacg agaaaacccc gtcattcacc gttaacggtg agaaacagtt
    19561 ttaccactgc tttggatgtg gcgcgcacgg caacgcgatc gacttcctga tgaactacga
    19621 caagctcgag ttcgttgaaa cggtcgaaga gctggcagca atgcacaatc ttgaagtgcc
    19681 atttgaagca ggcagcggcc ccagccagat cgagcgccat cagaggcaaa cgctttatca
    19741 gttgatggac ggtctgaata cgttttacca acaatcttta caacaacctg ttgccacgtc
    19801 tgcgcgccag tatctggaaa aacgcggatt aagccacgag gttatcgctc gctttgcgat
    19861 tggttttgcg ccccccggct gggacaacgt cctgaagcgg tttggcggca atccagaaaa
    19921 tcgccagtca ttgattgatg cggggatgtt ggtcactaac gatcagggac gcagttacga
    19981 tcgtttccgc gagcgggtga tgttccccat tcgcgataaa cgcggtcggg tgattggttt
    20041 tggcgggcgc gtgctgggca acgatacccc caaatacctg aactcgccgg aaacagacat
    20101 tttccataaa ggccgccagc tttacggtct ttatgaagcg cagcaggata acgctgaacc
    20161 caatcgtctg cttgtggtcg aaggctatat ggacgtggtg gcgctggcgc aatacggcat
    20221 taattacgcc gttgcgtcgt taggtacgtc aaccaccgcc gatcacatac aactgttgtt
    20281 ccgcgcgacc aacaatgtca tttgctgtta tgacggcgac cgtgcaggcc gcgatgccgc
    20341 atggcgagcg ctggaaacgg cgctgcctta catgacagac ggccgtcagc tacgctttat
    20401 gtttttgccg gatggcgaag accctgacac gctggtacga aaagaaggta aagaagcgtt
    20461 tgaagcgcgg atggagcagg cgatgccgct ttccgcattt ctgtttaaca gcctgatgcc
    20521 gcaagttgat ctgagtaccc ctgacgggcg cgcacgtttg agtacgctgg cactgccatt
    20581 gatatcgcaa gtgccgggcg aaacgctgcg aatatatctt cgtcaggaat taggcaacaa
    20641 attaggcata cttgatgaca gccagcttga acgattaatg ccaaaagcgg cagagagcgg
    20701 cgtttctcgc cctgttccgc agctaaaacg cacgaccatg cgtatactta tagggttgct
    20761 ggtgcaaaat ccagaattag cgacgttggt cccgccgctt gagaatctgg atgaaaataa
    20821 gctccctgga cttggcttat tcagagaact ggtcaacact tgtctctccc agccaggtct
    20881 gaccaccggg caacttttag agcactatcg tggtacaaat aatgctgcca cccttgaaaa
    20941 actgtcgatg tgggacgata tagcagataa gaatattgct gagcaaacct tcaccgactc
    21001 actcaaccat atgtttgatt cgctgcttga actgcgccag gaagagttaa tcgctcgtga
    21061 gcgcacgcat ggtttaagca acgaagaacg cctggagctc tggacattaa accaggagct
    21121 ggcgaaaaag tgatttaacg gcttaagtgc cgaagagcga tcgggaagcc cccgacagcc
    21181 gcactgagag gcagcggcaa atatataagt acgccctcgt aattatcgtt ggcggtaaac
    21241 aaccgttgga tttcagcgtt aacggctgaa ggacatcggg tcaatcgccc aacaccaacc
    21301 tcatgaaata agtgtggata ccgtcttatg gagcaaaacc cgcagtcaca gctgaaactt
    21361 cttgtcaccc gtggtaagga gcaaggctat ctgacctatg ccgaggtcaa tgaccatctg
    21421 ccggaagata tcgtcgattc agatcagatc gaagacatca tccaaatgat caacgacatg
    21481 ggcattcagg tgatggaaga agcaccggat gccgatgatc tgatgctggc tgaaaacacc
    21541 gcggacgaag atgctgccga agccgccgcg caggtgcttt ccagcgtgga atctgaaatc
    21601 gggcgcacga ctgacccggt acgcatgtac atgcgtgaaa tgggcaccgt tgaactgttg
    21661 acccgcgaag gcgaaattga catcgctaag cgtattgaag acgggatcaa ccaggttcaa
    21721 tgctccgttg ctgaatatcc ggaagcgatc acctatctgc tggaacagta cgatcgtgtt
    21781 gaagcagaag aagcgcgtct gtccgatctg atcaccggct ttgttgaccc gaacgcagaa
    21841 gaagatctgg cacctaccgc cactcacgtc ggttctgagc tttcccagga agatctggac
    21901 gatgacgaag atgaagacga agaagatggc gatgacgaca gcgccgatga tgacaacagc
    21961 atcgacccgg aactggctcg cgaaaaattt gcggaactac gcgctcagta cgttgtaacg
    22021 cgtgacacca tcaaagcgaa aggtcgcagt cacgctgccg ctcaggaaga gatcctgaaa
    22081 ctgtctgaag tattcaaaca gttccgcctg gtgccgaagc agtttgacta cctggtcaac
    22141 agcatgcgcg tcatgatgga ccgcgttcgt acgcaagaac gtctgatcat gaagctctgc
    22201 gttgagcagt gcaaaatgcc gaagaaaaac ttcattaccc tgtttaccgg caacgaaacc
    22261 agcgatacct ggttcaacgc ggcaattgcg atgaacaagc cgtggtcgga aaaactgcac
    22321 gatgtctctg aagaagtgca tcgcgccctg cagaaactgc agcagattga agaagaaacc
    22381 ggcctgacca tcgagcaggt taaagatatc aaccgtcgta tgtccatcgg tgaagcgaaa
    22441 gcccgccgtg cgaagaaaga gatggttgaa gcgaacttac gtctggttat ttctatcgcc
    22501 aagaaataca ccaaccgtgg cttgcagttc cttgacctga ttcaggaagg caacatcggt
    22561 ctgatgaaag cggttgataa attcgaatac cgccgtggtt acaagttctc cacctacgca
    22621 acctggtgga tccgtcaggc gatcacccgt tctatcgcgg atcaggcgcg caccatccgt
    22681 attccggtgc atatgattga gactatcaac aaactcaacc gtatttctcg ccagatgctg
    22741 caagagatgg gccgtgaacc gacgccggaa gaactggctg aacgtatgct gatgccggaa
    22801 gacaagatcc gcaaagtgct gaagatcgcc aaagagccaa tctccatgga aacgccgatc
    22861 ggtgatgatg aagattcgca tctgggggat ttcatcgagg ataccaccct cgagctgccg
    22921 ctggattctg cgaccaccga aagcctgcgt gcggcaacgc acgacgtgct ggctggcctg
    22981 accgcgcgtg aagcgaaagt tctgcgtatg cgtttcggta tcgatatgaa caccgaccac
    23041 acgctggaag aagtgggtaa acagttcgac gttacccgcg aacgtatccg tcagatcgaa
    23101 gcgaaggcgc tgcgcaaact gcgtcacccg agccgttctg aagtgttgcg tagcttcctg
    23161 gacgattaat cggtaggccg gatcaggcgt tacgccgcac ccggcactag tccctctgca
    23221 caaacgccac ctttt
//
LOCUS       DAEEKQ010000054        22883 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.054, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000054 DAEEKQ010000000
VERSION     DAEEKQ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22883
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.054"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            103..576
                     /locus_tag="KLK57_002007"
     CDS             103..576
                     /locus_tag="KLK57_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HBE0892375.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            671..1195
                     /gene="sat2"
                     /locus_tag="KLK57_002008"
     CDS             671..1195
                     /gene="sat2"
                     /locus_tag="KLK57_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HBE0892376.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            1253..2041
                     /gene="aadA1"
                     /locus_tag="KLK57_002009"
     CDS             1253..2041
                     /gene="aadA1"
                     /locus_tag="KLK57_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBE0892377.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            2117..2614
                     /locus_tag="KLK57_002010"
     CDS             2117..2614
                     /locus_tag="KLK57_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892378.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            2675..3046
                     /locus_tag="KLK57_002011"
     CDS             2675..3046
                     /locus_tag="KLK57_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892379.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            3456..3833
                     /locus_tag="KLK57_002012"
     CDS             3456..3833
                     /locus_tag="KLK57_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892380.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            3856..4008
                     /locus_tag="KLK57_002013"
                     /pseudo
     CDS             3856..4008
                     /locus_tag="KLK57_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4064..5680)
                     /locus_tag="KLK57_002014"
     CDS             complement(4064..5680)
                     /locus_tag="KLK57_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HBE0892381.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(5681..7207)
                     /locus_tag="KLK57_002015"
     CDS             complement(5681..7207)
                     /locus_tag="KLK57_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HBE0892382.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(7210..8877)
                     /locus_tag="KLK57_002016"
     CDS             complement(7210..8877)
                     /locus_tag="KLK57_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBE0892383.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(8874..10982)
                     /locus_tag="KLK57_002017"
     CDS             complement(8874..10982)
                     /locus_tag="KLK57_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBE0892384.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(10969..11790)
                     /locus_tag="KLK57_002018"
     CDS             complement(10969..11790)
                     /locus_tag="KLK57_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HBE0892385.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(11949..13778)
                     /gene="glmS"
                     /locus_tag="KLK57_002019"
     CDS             complement(11949..13778)
                     /gene="glmS"
                     /locus_tag="KLK57_002019"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HBE0892386.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLDGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(13940..15310)
                     /gene="glmU"
                     /locus_tag="KLK57_002020"
     CDS             complement(13940..15310)
                     /gene="glmU"
                     /locus_tag="KLK57_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HBE0892387.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKVGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(15661..16080)
                     /gene="atpC"
                     /locus_tag="KLK57_002021"
     CDS             complement(15661..16080)
                     /gene="atpC"
                     /locus_tag="KLK57_002021"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HBE0892388.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(16101..17483)
                     /gene="atpD"
                     /locus_tag="KLK57_002022"
     CDS             complement(16101..17483)
                     /gene="atpD"
                     /locus_tag="KLK57_002022"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HBE0892389.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(17510..18373)
                     /gene="atpG"
                     /locus_tag="KLK57_002023"
     CDS             complement(17510..18373)
                     /gene="atpG"
                     /locus_tag="KLK57_002023"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HBE0892390.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKSVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(18424..19965)
                     /gene="atpA"
                     /locus_tag="KLK57_002024"
     CDS             complement(18424..19965)
                     /gene="atpA"
                     /locus_tag="KLK57_002024"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HBE0892391.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(19978..20511)
                     /gene="atpH"
                     /locus_tag="KLK57_002025"
     CDS             complement(19978..20511)
                     /gene="atpH"
                     /locus_tag="KLK57_002025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HBE0892392.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(20526..20996)
                     /gene="atpF"
                     /locus_tag="KLK57_002026"
     CDS             complement(20526..20996)
                     /gene="atpF"
                     /locus_tag="KLK57_002026"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HBE0892393.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(21058..21297)
                     /gene="atpE"
                     /locus_tag="KLK57_002027"
     CDS             complement(21058..21297)
                     /gene="atpE"
                     /locus_tag="KLK57_002027"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HBE0892394.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(21344..22159)
                     /gene="atpB"
                     /locus_tag="KLK57_002028"
     CDS             complement(21344..22159)
                     /gene="atpB"
                     /locus_tag="KLK57_002028"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HBE0892395.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(22168..22548)
                     /gene="atpI"
                     /locus_tag="KLK57_002029"
     CDS             complement(22168..22548)
                     /gene="atpI"
                     /locus_tag="KLK57_002029"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HBE0892396.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAYTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         5882 a         5878 c         5204 g         5919 t
ORIGIN      
        1 aagtctattt aatacaagtg attatattaa ttaacggtaa gcatcagcgg gtgacaaaac
       61 gagcatgctt actaataaaa tgttaacctc tgaggaagaa ttgtgaaact atcactaatg
      121 gtagctatat cgaagaatgg agttatcggg aatggccctg atattccatg gagtgccaaa
      181 ggtgaacagc tcctgtttaa agctattacc tataaccaat ggctgttggt tggacgcaag
      241 acttttgaat caatgggagc attacccaac cgaaagtatg cggtcgtaac acgttcaagt
      301 tttacatctg acaatgagaa cgtattgatc tttccatcaa ttaaagatgc tttaaccaac
      361 ctaaagaaaa taacggatca tgtcattgtt tcaggtggtg gggagatata caaaagcctg
      421 atcgatcaag tagatacact acatatatct acaatagaca tcgagccgga aggtgatgtt
      481 tactttcctg aaatccccag caattttagg ccagttttta cccaagactt cgcctctaac
      541 ataaattata gttaccaaat ctggcaaaag ggttaacaag tggcagcaac ggattcgcaa
      601 acctgtcacg ccttttgtac caaaagccgc gccaggtttg cgatccgctg tgccaggcgt
      661 taggcgtcat atgaagattt cggtgatccc tgagcaggtg gcggaaacat tggatgctga
      721 gaaccatttc attgttcgtg aagtgttcga tgtgcaccta tccgaccaag gctttgaact
      781 atctaccaga agtgtgagcc cctaccggaa ggattacatc tcggatgatg actctgatga
      841 agactctgct tgctatggcg cattcatcga ccaagagctt gtcgggaaga ttgaactcaa
      901 ctcaacatgg aacgatctag cctctatcga acacattgtt gtgtcgcaca cgcaccgagg
      961 caaaggagtc gcgcacagtc tcatcgaatt tgcgaaaaag tgggcactaa gcagacagct
     1021 ccttggcata cgattagaga cacaaacgaa caatgtacct gcctgcaatt tgtacgcaaa
     1081 atgtggcttt actctcggcg gcattgacct gttcacgtat aaaactagac ctcaagtctc
     1141 gaacgaaaca gcgatgtact ggtactggtt ctcgggagca caggatgacg cctaacaatt
     1201 cattcaagcc gacaccgctt cgcggcgcgg cttaattcag gagttaaaca tcatgaggga
     1261 agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
     1321 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
     1381 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
     1441 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
     1501 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
     1561 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
     1621 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
     1681 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
     1741 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
     1801 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     1861 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     1921 catacttgaa gctaggcagg cttatcttgg acaagaagat cgcttggcct cgcgcgcaga
     1981 tcagttggaa gaatttgttc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     2041 atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     2101 agagctgggg aagactatgc gcgatctgtt gaaggtggtt ctaagcctcg tacttgcgat
     2161 ggcatcgggg caggcacttg ctgacctgcc aattgtttta gtggatgaag ctcgtcttcc
     2221 ctatgactac tccccatcca actacgacat ttctccaagc aactacgaca actccataag
     2281 caattacgac aatagtccat caaattacga caactctgag agcaactacg ataatagttc
     2341 atccaattac gacaatagtc gcaacggaaa tcgtaggctt atatatagcg caaatgggtc
     2401 tcgcactttc gccggctact acgtcattgc caacaatggg acaacgaact tcttttccac
     2461 atctggcaaa aggatgttct acaccccaaa aggggggcgc ggcgtctatg gcggcaaaga
     2521 tgggagcttc tgcggggcat tggtcgtcat aaatggccaa ttttcgcttg ccctgacaga
     2581 taacggcctg aagatcatgt atctaagcaa ctagcctgct ctctaataaa atgttaggcc
     2641 tcaacatcta gtcgcaagct gaggggaacc actagtgtca tacgaacctc caagagacgg
     2701 ttacacaaac gggtacattg ttgatgtcat gtatgacaat cgcccaagta agtatccagc
     2761 tgtgttcaga acgtacgtcc gaattccaga ctatgtcgaa ctagataacg cggaggagct
     2821 ggccgacgca atcattcgag aggttgagtt aaaggaggca tggctcacaa gggtcttggg
     2881 gaactcagcc gatcctgacg cttctgtggg caagtgggac cggcgcctag cgcagtgcgg
     2941 tggtattgat gagatgtcaa gtggggtgca ggcaacatac ttcagtccaa acggtcagct
     3001 ggtcggctta gtggcataca ttgatgccga atggatggat gaatagctct gacgttacga
     3061 ttcaggtgag acaaccagat gggcattgcg tacaagctca gttgccaccg atgtggctac
     3121 gagtctgatc ttctttacct aggccaaggt atggcgatgc ttcctgagca aatagtcgga
     3181 acgtgcacat gctgcgacaa tctaacgacg atttctataa atgaaagcta cggagtatgc
     3241 tcactttgtg gggctaagga tcgtgtgata tttacaagct atcagcatga ggcaaggcca
     3301 agaaatccct gctatctaga gagaagtttc aaatatcaat gcccaagatg ccatgccttc
     3361 agtatggagc ctccggatct accggagatg ctttgggact aatgctccgc tttgcctcgg
     3421 gtatccgttc gacctatgtt taagaggaaa cgcacgtgtc tgccgccaaa gctcttgcct
     3481 atgcactttg tgacaagttg gaaagcaaaa aagttgatga agtcgtacga aatcttgttt
     3541 ctagcggctg gaatattagg gaagtggcct gggaagcttc gtcctttgat catgaaatgc
     3601 cctactttga cacggggcgg gacttcgata ttggatttcc tgacacgctg tcatccaaga
     3661 ttgagttttt gaccgcgaat ggatcaatcg tccaagcacg tgctcgcctg cacttcaagc
     3721 aggctctaat ttttgccaag gcgtccaaac atgtcgccgc gctcaagaac tcattggatt
     3781 actactatgg atcgtctgta atcccccctc aaaaccacgg aggctatttg tagaaaattc
     3841 cattaatatg aactcaaggt gttccctacg gacgaatcag cgatgaaggt ggtgtacctg
     3901 gctatccagg cggcgaccaa gaaatggacg atgccgatcc gcaactggaa gccggccatg
     3961 aaccgtttta tgatcgaatt tggtgaccgt ttaaatggcc acctttaact gaccggcaaa
     4021 agcacttaca caaaagggtt tacaggctct ttcagtcatt cctttaatgc gtaaattgct
     4081 ctctaaccac tctctcagcc cacccttcca tatcctctct cgatacttgc agctccccca
     4141 acccctttgg atgattcacc cctctatgcc cgtcttgtcc atataattga tcaaacaaac
     4201 tatttggcca attcaatgag ctcttcacca ctccacgccg tatcttttca aaatcattcc
     4261 gccatgtttc gctatcaacg ccactcaaca ctttggtaga aagcattttc accccatccg
     4321 atgcatccac ctctagcaat acaaattcac tgccattacg ttgcacccca acggctttaa
     4381 tcactcttgg tgagccatct ttacacagat gtaatcggct acgcccaact tttggcaaaa
     4441 ccaaggtttc ttcaaaaagc acccgacatg caaatttagt ttttagaatt gaaagtagct
     4501 catcaaaagc agcaaatcga ttagcaaaaa tagagttgta attggttgca tcctgcttcc
     4561 cacccacatc tgccgctgct aggacaccac ctaagtgtgg ctcatcagta ctaaccaaat
     4621 tactattttc acttgttgtt gcttcttctt tttctaaaat agtttggcta ctttttcttg
     4681 ccctgcgaga tttttcaatt cgactaggtc tactaaaact tatccatgtc ggctcagcct
     4741 ctatgactcg tgtgtctgtg tctatattgg ctgtttcctc atcgtccaac tgtagatgct
     4801 catcatcaac aactgactct gttgaaaccg ctatgtgctg cgtactacca tcacctgcct
     4861 ccttttcctg aaaagaggca tggctaatag ctgttgtgct aggaagcata gcgttgattt
     4921 ctaaacctac tatttcctca actaaataat ccttatcctc gttagaagaa cgtcctttta
     4981 catgtaattt ccaaccctgc atgggcggag ggtcaaagct aaagcaccag ctttcaacgc
     5041 cgttcttaat ttctctattt tgttgatagt gcctaaaaat actttcatac gaatccataa
     5101 catcttgatc cgaaaacaac caaaccaaaa gctgcactac ggccgactgc tctaacgccc
     5161 ctttaggaaa cgatgaactg ggtaagatcc ttatctctaa atgatctcgc tcaacctcat
     5221 actgaacgtc gaactcttgc tgtaaagcgg tactgctcaa acagcttcga cagaaatagg
     5281 agttaattaa aaataatgac cgagctagct ctaactgtgg gatgtgataa cgagtcttgc
     5341 tatatggtgt ttcagcttcg taaataaatg aactctgcgc tccatccttg ttgcgtacgg
     5401 aagcaaagga cgagagatta ggaaaatcga ttattttggc tcgttgaaga tccgatgctt
     5461 caaactcaat cacacgatcc gccttattta tttgctttgt tgtactgtta accaccttag
     5521 ctcgacttaa taaaggcaaa tgagtaaatc gagtccattt ccgttcttgc attggattaa
     5581 accaaacaaa aatacgccac tccttatgac ccgagttacg gaataaatga ccaatatgta
     5641 caacctgcac attgtcatta aatgtagcta gcctaaccac tcactcttcc ccataaacca
     5701 tttccaagaa tctttgtgct tcctcagtta tccgctcttt agataacgtt gcacttctta
     5761 ataatcgcca gcgcctaagc tcaacatctt cctgtttaag cttaataaaa gcttggctaa
     5821 tccgtctaat ttgataatct tccacactct ctgagtaacg ctttaagcaa agcgctacca
     5881 aaggcagttt ctgtagattt tttgcaagag aggttccgtt aggagtttgc tttaacagcc
     5941 agctcgatgt cgctcttggg tgatcaaggc tactatctag acgctttatg attcttaata
     6001 gttgtcgtac agcaattcga tctctttggt tccaatcaac tctaggggcg ggtgccagac
     6061 gctcttgttg atgctgttga ttccagtgaa ctagccaatc cctgtcatgt cggtaaagcc
     6121 aagcgtatag caccccaccc tctaaagact gtcttgccgc cttaattcct tggtatttat
     6181 gcactagttg ctgccagtct ttatgcttaa cagataaatc ttcagagtta ggttgcacag
     6241 actggctaac aggtcttgtc gttatagagt gctcagtaag agcacttgcc tgctgtagcg
     6301 cttctataac cgttagtttt ggcaataagg cttgccacac aatactatgc tgtaaataac
     6361 taaaggcttt tctatgctta cggaatatac ttttcagcca acacgtgtcc ttgttctctg
     6421 ccaactttaa atccagtttc tctagtgcct catcactaaa agtctgcctc actctctccg
     6481 ccaccaagtc atgacgaatg tgcttgcttt tggttagccc tagatcctgc gctaagcgct
     6541 gataaaacag cgtccactgc tcaaggcttg gggaaagctc ttgcgctcgt ggagcatcta
     6601 acagaggggc tatataagca gctagtgctg ttaattgaga tagggagtct ttggggtagt
     6661 ctgaaagcag ctcagtatga cccaaagccc aaaattgatg tcggtgatca tctacagctc
     6721 tatcaaagaa gactaaagca ccgtgttttg gacaatatgg caaagcgggc aaataccaat
     6781 ctcgttgcca aaaggcttcc ccatacctat ttagctgaag agcaacgcaa tcagggcagt
     6841 atctaaagcg gttatcgctc ttaactctag aagcagcgac tcctagcatt aaatgcaccg
     6901 caccttgcgc ttggtactcc attaaccgaa tagcttcgtc tcggcgctcc ttgcctacaa
     6961 acggagcata taaagggaat aaggtatgct cataaataag ctgctgaaca gcgtaacgtc
     7021 ctgtttgatg tagatgtctt gctatcacac ctaaatgcga gggcagacct aaggtagcga
     7081 ccaccttgcg gttgccatac acctcatcca acagctgctt aggactaaca atcccttgat
     7141 aaacgcctgc ccgtgcaata gtgctataaa tcagctcatt cgagtacgga acaggaaagt
     7201 ttctcatggc taacctgctt gtttaaataa gctcgccata tccactatta cccctttccc
     7261 ttttaaccgt tcatgcatgg ttttttcagg ttggcgttgt gaatagatat aacgtaaatc
     7321 cgtatcaggc aagctatccc agtcgctggg cttgactacc tttatagccg aaaccttttt
     7381 actcttggag ggcttttcta gttctgacac taccgtttcg ccttccatca accactgcaa
     7441 aacaagaggc agtaactttt gtcttgtcat cgttggattc tggctaaacg cttttttaat
     7501 agtgggaatt aacaggcttg aatcgtaatc ctctttcagc atcagatata aatgacgctg
     7561 atcttcggta tctaactctt gaagggcttt ttcttctggt gtttgttctt gtatcgctgc
     7621 gatatctagc tgaagttgga ttaaccgttt atcaatctcg ggaacgacta gatcagaata
     7681 acgagcaatg cgttctggga tacccgagcg taatgcctct agcatggggt gcacaggctt
     7741 taactcatct tgatacactt gccgcaataa accagcggta atacgctcat tgcctaaagc
     7801 tagcgcacgg agctgagcga gtacaaaaag ttttactaca atgtccatca ctccttggct
     7861 tagctcatac cacacatcac ggacctcatc cgataacagc gcatctttgc gttgtaaaag
     7921 ctgtaattgc caaagattat ccgtaaaagc gatccactct tgattgggct ttccacgttg
     7981 cgtttgttgt ataggatccc agaatatagc tccaaaccct gcccctctac gtgcagaccg
     8041 caaatcagcc tcaaaaatct ctcgtgcttt aggggtacca atcaacatca ctggtacgcc
     8101 aataatattc accatcgtca caaaaaagtt cagcatctct tgagatccac ccgaacgaga
     8161 gcggcttaaa tgctgaattt catcaataac caacaaccct aaagcatgtg cattggctat
     8221 ttgcgacatc aaagccaaca tggtttctat accatgacgt tttaagccat aacgacgctc
     8281 atagttcgag cccaaggctc gatccaacgc tctgaaaaaa ttcaagcaga tttcttttag
     8341 cgaaccatta tgcgagcagt ctattttcaa atacaccacc tgctctacat tgagttcacg
     8401 atggtaaatc acctgaggat acgtggctag aatacgatga agagaggtcg tcttcccact
     8461 accagaacaa ccaattaaca ataagctttg tgccgtagat cgtgcctcct caaagcgaaa
     8521 tgtctccaac tctcccgttt gaacacgctc ataaccattt tgtaaatgct tttgtaaatc
     8581 tcctgtttta ggatttctgc ctacgtagcc acctcgaatc atgaccgaaa tacgctcact
     8641 tagtagcaaa tgcgtaccta atggctgaaa atagtcatct ggaatacgac aaatggtatg
     8701 agctctgata acacgggact tttgcaagtc agaggaagta agctgtaaag aggatttcag
     8761 tgatgcagca ctattcactg actcttgtaa tggtggtaag gcctcgataa aaggattatc
     8821 acgataagcc tctacccccg tatcacgata aactgcttga atccgggtag cactcatgac
     8881 tcatcctttt ctggtggatc ctgaaataat tcaggcacgt atgtgggtag gctgtaatct
     8941 tcttgatcat ccgcttccac tgcgttgaaa ggaataactt tagcctcatc acctgaagag
     9001 cttggcttca aatgctccgc acgtttttta cgctccgagg tcacggcttc ttttttatta
     9061 gttttaatct gcttaatgcg tgttgatttg ggctcagtag tactgggcgt taacttattc
     9121 gctttctgaa tggtttgctg aataaacgcc tcaagctccc tgcgtttagt taactcatcc
     9181 tgcttcgcat tggctttatt gtgtttttct tgtgcttgta tatcccaaac ctcccaaaat
     9241 gagagacctt taaactgccg actacgttcc gtcagattac agcgccaaaa tacacggctg
     9301 ccaacttgcg gaaacaaata aatcgtatca accagcactg ggtcataagc cgcttctaaa
     9361 tgttgaggtc tagctatatc agtgctccgc tgcaaccaac cctcacgcag aatctctgac
     9421 cccgagtaat acaaacccca caaattaacg ccaaatgaag aaatagagac ctttcggcga
     9481 ggcagtaacg ctactcgcaa ctgctcttgc tccacagccc ttaaactacc tgtacgatgc
     9541 tgcatacccc attgccatag ctggacagga atagacggta aatctgtagg aaaatcagca
     9601 tctcgatcgt atttatccat caccagatgg ttatttctga ataagatcgt acgcaaaata
     9661 atttgtgtga actcaaatac cgacagagat gcatctaacc tatagtctgt ttcaccatgg
     9721 cttttgatcc gactgccctc tacaatgcca ggtgcaaagg acttaaactc ggcttgtagt
     9781 gttctaaaag tgctttccac tatgccttta gcatcgccac gtctaggtgg agcactttcc
     9841 actcgcacat taaaactaga aactaaggct tcgacctgat gactcattaa ttcgccacgg
     9901 tccgctagca acacatctgg caaacctaca cacggccagt ctgagctact aatctcaata
     9961 tcatgctggg cacaaatggc cgttttgtca gagcaagcat ttacaaaagc ctgcatcgcc
    10021 accacataag acggattttc aaagccgata taaaagcccg tgatcatccg actaaacaca
    10081 tcaatcacaa tgtaaagcgt tggtcttcct atgatttttt ggcgatcatg atgatccact
    10141 aaataaatat cagcaatcgt ggcatcaatc tcataacgac tcccagggcc taacgcctga
    10201 gaagtggctg tactacttaa gggtcgtacg tcttttttat atacccctgc tttaactcta
    10261 gactttaagc gctgagcttt agggtattct cgatcataaa aataacgaaa ctgacgtagt
    10321 gttgggtaat cctcttgggg aatgcgagga aaatactgag caaacaagtc cacaaatcgt
    10381 ctataggcaa cggtggtctt tgtacctttt tgatttaaca ggtgcttttc tatggtcaac
    10441 ctaaaaaggc gttcaatctc gggcgttacc ttggttcctt cacccttacc atattctctg
    10501 gctcggccaa tctttgctgt tcctgtcgct gaacgtcttt cccctggtgc accgctgttt
    10561 ttgtagtcag gaattaatgc attaggcgtt tgaccacgct gccagtaacg gcgtaacaac
    10621 ttataaactg tagccttagt aaccttatgt tcttggacca catgctcaac gagttcgctt
    10681 ctgactttag ggtcgaaacg atccttacta ttaataatag gaagaatttt tcgataatcc
    10741 tcctcgcgct tctggaagct gacagaatct acagacggct cttctaaatc aagatgtaca
    10801 taagggtcat cagttctaac taagcggcct tcatctaaat actgcattag caacaactca
    10861 gccctagctt gtggaactcc tttatcagcg cttatttgca tccagacaac ttggccatcc
    10921 tctatagcca aaatgcgata cggatcatta tcaaatagca caacctcatt aatttgccac
    10981 atagcgcaac tcctccatat tcactacttg gctaatacag agatctgcac acttatttgc
    11041 cctgaaagac ttataaatat tgaacttaat aaaaccattt gcggttaagg ctcgtatctc
    11101 actcaatgtt ttgcctaact ccaaatcata agcaatatca acctgcttac agacattgat
    11161 aatgttttca tctccttttt cttgcaggat atgggccaat ggggatagtt gtgctaaaag
    11221 ctccgcagaa acttcttctg ttttcactga ataaagccat tcaatatttt cttttactac
    11281 gggatttatt tctttatcag taaaaatgaa ccaaggaatt tgcttttgct gccaatagcg
    11341 acgctctagt tctagttttt ctaaggtacg ctcgtcttgt aaggctgctg caggtttgac
    11401 ttgaatagca aactgctcaa aaggaccatc tttgcagtcc actaaaaaat cagtagacat
    11461 aacctgatct acaccacgaa taacaggatg cttaatacca ctatctattg caatctgcct
    11521 ggtatcacta ggtaataagg ggaactgctc gcgtatatct agcacgctgc tctcccactc
    11581 aagactgaga aaaacagcaa gctctaagtc agatagcaaa tgatggactc gtcccgtctt
    11641 atgagaataa atacggtggg aacgacctga agaaggaact tcttgtactg ttagccatgg
    11701 aatatagtct ttaccatgcc cttggccacg cccctcttta atacgacggg caatttgcac
    11761 ttcagaaaat gaagagtttg ctttagccat aacaaaagtc cagtatgctt tttcacagca
    11821 taactggact gatttcagtt tacaactatt ctgtctagtt taagacttta ttgtcatagt
    11881 ttagatctat tttgttcagt ttaagacttt attgtccgcc cacagccctt ttttatctat
    11941 gatagtgatt actcaaccgt aaccgatttt gccaggttac gcggctggtc aacgtcggtg
    12001 cctttgatca gcgcgacatg gtaagccagc agctgcagcg gaacggtgta gaagatcggt
    12061 gcaatcacct cttccacatg cggcatctcg atgatgtgca tgttatcgct acttacaaaa
    12121 cccgcatcct gatcggcgaa gacatacaat tggccgccac gcgcgcgaac ttcttcaatg
    12181 ttggatttca gtttttccag caattcgttg ttcggtgcaa cgacgataac cggcatatcg
    12241 gcatcaataa gcgccagcgg accgtgtttc agttcaccag cagcgtaggc ttcagcgtga
    12301 atgtaggaga tctctttcag cttcaatgcg ccttccagtg cgattgggta ctgatcgcca
    12361 cggcccagga acagtgcgtg atgtttgtca gagaaatctt ctgccagagc ttcaatgcgt
    12421 ttgtcctgag atagcatctg ctcaatacgg ctcggcagcg cctgcagacc atgcacaatg
    12481 tcatgttcaa tggaggcatc cagacctttc aggcgagaca gcttcgccac cagcatcaac
    12541 agcacagtta actgagtggt gaatgcttta gtggacgcca cgccgatttc cgtacccgcg
    12601 ttggtcatca gcgccagatc ggattcgcgc accagagaag aacccggaac gttacagatt
    12661 gccagcgaac caaggtaacc cagctctttc gacagacgca ggcccgccag ggtatccgcg
    12721 gtttcgccag actgtgacaa ggtgatcatc aggctgttac gacgcacggc agatttacga
    12781 tagcggaatt cagaggcgat ttcgacgtcg cacggaatac ctgccagcga ttcaaaccag
    12841 tagcgggaaa ccataccgga gttataagaa gtaccacagg cgaggatctg aatatgctca
    12901 accttcgaca gcagttcgtc ggcgttcggt cccagctcgc ttaaatcaac ctgaccgtgg
    12961 ctgatgcgcc cggtaagggt gttttttatc gcgttcggct gttcgtagat ctctttctgc
    13021 atgtagtgac ggtaaatacc tttatcgccc gcgtcatatt gcagattgga ttcgatatcc
    13081 tgacgtttta cttccgcgcc agttttatcg aagatgttta ccgaacggcg agtgatttcc
    13141 gcaatatcgc cctcttcaag gaagataaag cgacgggtca ccggcaacag cgccagctgg
    13201 tcagaagcga taaagttttc gcccataccc aggccaatca ccagcggact accagaacgt
    13261 gccgccagca gggtatccgg gtgacgggag tccatgatca ctgtaccgta cgcaccgcgc
    13321 agctgcggga tagcacgcag aacagcctca cgcagagtcc cgccttgttt cagctcccag
    13381 ttcaccagat gggcaatcac ttcggtgtcg gtttcagaaa cgaaggtata gccacgcgct
    13441 tttaactctt cacgcagcgg ttcatggttt tcgatgatgc cgttatgcac caccacaatg
    13501 tgttcagaaa catgcggatg cgcgttagct tctgaaggtt caccgtgggt cgcccagcga
    13561 gtgtgagcaa taccggtgcc gccatccaga ggatgttctt ccgctgcctg agccagcatc
    13621 tggactttac cgaggcgacg caggcgggtc atatgacctt ccgcatcaac aacggccaga
    13681 ccggcagagt catatccgcg gtattccaga cgacgtaaac cttcaagaag gatttctgct
    13741 acatcacgtt gcgcgatcgc gccaacaatt ccacacatag tttttgattc cgatttatat
    13801 cgttgtcggt caacctgtat gcccgttttt tcgggcgccc cgagccttgt agagagtggg
    13861 gttattttta tagttactgc ttgtgggagg gagattatgt tatctcctca tcccagatga
    13921 ccggattacc cggccagaat tactttttct ttaccggacg acgccagcct tctttctgag
    13981 tctgcggcac acggctgata gctaatgcat tttcgccgac attacgcgtc acagttgtac
    14041 ccgcagcaat ggtcgcgcct ttgcctactg ttaccggggc caccagctga gtgtcggaac
    14101 cgacaaacac atcgtcgccg ataatggtct taaatttatt cgcaccatcg tagttgcagg
    14161 taatggttcc cgcgccgatg ttaacgttat cgccaatttc cgcatcgccc aggtaagtca
    14221 gatgcccgac tttcgagcct ttacccagac gcgctttttt catctcaacg aagttaccaa
    14281 cgtgtgcacc ttccagcaac tcagcaccag gacgcaaacg agcaaacggg ccaatagtac
    14341 aggccgctgc cagattcgcg tcttccacaa cggtatacgg actgatttcg caatcatcgc
    14401 caatcacgct gtttttaatc acgcaaccgg tgccgatttt cacgcgatga ccgagagtca
    14461 cgttgccctc gatgataacg ttagtatcaa tttcaacatc gcgcccgtga gtaagcgtac
    14521 cgcgcagatc aaaacgcgcc ggatcgcgca gcataacgcc tgctaacagc agtttttcag
    14581 cctgttcgga ctggtaaacg cgctccagac gggagagttg caggcggtta ttcacacctt
    14641 ctacttcgct taaacgttgc ggatgaacgg cgacgatttc acgcccttcc tgatacgcca
    14701 gcgcaataat gtcggtgatg tagtattcgc cctgagcatt attgttggtc agcttcgcca
    14761 gccagcgttt catatctgcg ccgttagcga tcagaatgcc ggtgttgatc tcctgaatct
    14821 gacgctgctc gtcggtggca tctttgtgct caacaatgcc ggtaactttg ccattttcac
    14881 gggtgatacg tccataaccg gtcggatcat ccagtttcac cgtcagcaga ccaatgccac
    14941 cctgcggttt agcatcacgc aggcgctgga gtgtttcgac agagatcagc ggcacgtcgc
    15001 cgtagagcat taaaatgtct tcatcatcgg caaagaaagg tgcggcctgc tgcattgcat
    15061 gacccgtacc cagctgctct gcctgaagca cccagttaag gttgtcgtct ttcagcgcct
    15121 gtttaagcag atcgccgccg tgaccgtaca ccaagtgaac gtgcgctgcg cctaattcat
    15181 tcgcagcatc aatgacatgc tgaaccatcg ctttcccggc aagggtatgc agcactttcg
    15241 gaagatcgga atacatgcgc gtgcctttgc ctgcggcaag gatcactacg ctcatagcat
    15301 tattcaacat acgcgtcctg actgtaattt gagaacgaat ttaaaccgct tcaccttgaa
    15361 aaaactacat ttttttcatc gtgaaatgga cagaggataa attgttcaat aacggattat
    15421 ccccgctgac gaaagtgcca ttttcgacca tcgtcacgcc ttttgtctgc agaaaattaa
    15481 ggcagtacct tcatttaagc gtcaggtgga tgtttttgct cttatttcga tcaatgaata
    15541 aacgggaaaa caggggtgtt ttatatcagc aggatctatg tgaacgctct tcaggacgga
    15601 tcacgcgtaa aaaaaagcca gcctgtttcc agactggctt ttgtgctttt caagccggtg
    15661 ttacatcgct tttttggtca actcgataac gcgcagctgc gcgatcgctt tggccagttc
    15721 cgcagacgcc tgagcgtaat ctacgtcgcc gtgagagctg ctaatgtgct cttcagcctt
    15781 acgtttcgct tccatggctc gcgcttcgtc gagatcctgg ccgcgaattg cggtgtcggc
    15841 cagaacggtc acgttgccag gctgcacttc aagaatgccg ccagacagat agataaactc
    15901 ttcgtgaccg tgctgtttca cgatgcgaat cataccaggc ttaatggcgg tgagcagcgg
    15961 tgcgtggcca gggtagatcc ccagttcacc ttcgctaccc gttacctgga ttttctcgac
    16021 cagaccagag aacatttgtt gctctgcgct gacgacgtcc aggtggtaag tcattgccat
    16081 atcaccctcc gattaaggcg ttaaagtttt ttggcttttt ccacagcttc ttcgatggaa
    16141 ccgaccatgt agaacgcctg ctccggcagg tgatcgtatt cgccttccat gatgccttta
    16201 aagccacgga tggtgtcttt cagggagacg tatttacccg gagaaccggt gaatacttct
    16261 gccacgaaga acggctggga caggaagcgc tggatcttac gagcacgcgc taccaccagt
    16321 ttgtcttctt cagacagttc atccataccc aagatggcga tgatgtcttt cagttcctga
    16381 taacgttgca ggatggactg aacgccacgc gcggtgtcgt agtgttcctg accaaccacc
    16441 agcgggtcca gctgacggct ggtggagtcc agcgggtcaa cggccgggta gatacccaga
    16501 gacgcgatct gacggctcag taccacggtt gcgtcaaggt gcgcaaaggt ggttgccgga
    16561 gacgggtcag tcaagtcatc cgcaggtacg tataccgcct gtacggaggt gatagaacca
    16621 gttttggtgg aggtgatacg ttcctgcaga acgcccatct cttccgccag ggtcggctga
    16681 taacctaccg ctgaaggcat acggcccagc agtgcggata cttccgtacc ggccagggtg
    16741 taacgataga tgttgtcaac gaacagcaga acgtcacgac cttcgtcacg gaatttctca
    16801 gccatggtca gaccggtcag agcaacgcgc agacggtttc ccggcggctc gttcatctgg
    16861 ccatacacca gggatacttt gtcgataacg ttggagtcgg tcatttcgtg gtagaagtcg
    16921 ttaccctcac gagtacgttc acctacgccc gcaaacacag agtaaccgga gtgctcgatc
    16981 gcgatgttac gaatgagctc catcatgttt acggttttac ctacacccgc accaccgaac
    17041 agaccaactt taccgccctt agcgaacgga cacatcaggt cgataacttt gataccggtt
    17101 tccagcagtt cctgagagtt tgacagctct tcgtaggaag gtgctgcgcg gtgaatcgcc
    17161 caacgctctt cttcaccgat ctcgcctttc atgtcgaccg gttcacccag tacgttcatg
    17221 atacggccca gagtcgcttt acctaccggg acttcgatcg ggtgttcgag gtcttttaca
    17281 tccagaccgc gacgcagacc gtcggaggaa cccattgcga tggtacgcac gataccgccg
    17341 ccgagctgct gctgaacttc cagcaccaga cgctcattac cattttgcac ctcaagagca
    17401 tcgtacacgc gcggtacggc atcctgaggg aattcgacgt caactacggc gccgattacc
    17461 tggacaatct ttccagtagc catcttaaat cctctacgaa ataacctgtt taaaccgcgg
    17521 cggcccccga gacgatctcg gtgagttcct gagtaatgct ggcctgacga gctttgttgt
    17581 ataccaactg cagctcttta atcaggctgc cgccattgtc ggtcgcggct ttcatcgcca
    17641 ccatacgggc ggcctgctcg ctggccaggt tttcaaccac gccctgataa acctgagatt
    17701 cgacataacg acgcagcagg gtatccagca acgccttcgg atcgggttcg tacaggtaat
    17761 cccaggattt atgtttcaga tcatcatcat ctgatgccgg taacggcagc agctggctga
    17821 tggtcggaac ctgagacatg gtgttaataa atttgttgct gacaatgtaa agcttgtcca
    17881 gacggccttc gtcgtaagcc tgcaacatca cttttaccgg accgatcagt tcggacaggg
    17941 aagggttatc ccccatgccg gtgacctggg caacaacatt gccgcccacg gagttgaaga
    18001 acgacacgcc tttcgagccg atcattgcga ggtcgcattg aacgcttttg tcggtccagg
    18061 tcttcatttc cgccagtagt tttttgaaca ggttaatgtt caaaccaccg cataaaccac
    18121 ggtcggtcga caccaccagg tagcccacgc gtttaacgtc gcggtcttcc aggtaagggt
    18181 gcttatattc cagattaccg tgtgcaaggt gaccaatcac tttgcgcatg gtttctgcat
    18241 aaggacggct ggccgccatg cgatcctgcg atttacgcat tttggaagcg gcgaccatct
    18301 ccatcgcttt agtgatcttt tgcgtgttct ggacgcttgc gatcttacta cgtatctctt
    18361 ttgcgccggc catgagcttc tcctcaatgc cttgcggcct gccctaaggc aagccgccag
    18421 acgttaccag gattgggttg ctttgaagga atcgaggatg cctttcagct tgccttcgat
    18481 ttcgtcgttg tagccaccgg tctggttgat ctcttgcatc aacggagcgt gatcacggtc
    18541 gacgtaagcc agcagagcgg cttcgaagct gccaattttc gacagttcaa catccgccag
    18601 gtaaccacgt tctgctgcga acagaaccag agactgctgc gcaacggaca tcggcgcata
    18661 ctgtttctgt ttcagcagtt cggtcacttt ctgaccgtgg tcaagctgct tacgtgttgc
    18721 atcgtcaagg tcggatgcaa actgagagaa cgctgccagt tcacgatact gtgccagagc
    18781 ggtacggata ccaccggaca gttttttcat gatcttggtc tgtgctgcac caccaacacg
    18841 ggatacggaa atacccgggt taaccgcagg acgaataccg gcgttgaaca ggttggtttc
    18901 caggaagatc tgaccatcgg taatggagat tacgttggtc ggaacgaacg cagaaacgtc
    18961 acccgcctga gtttcgataa tcggcagtgc ggtcagagaa ccggttttcc ctttcacttc
    19021 acctttggtg aaggcttcaa cgtattcggc gttaacacgt gcagcacgct ccagcagacg
    19081 agagtggagg tagaaaacgt cgcccgggaa tgcttcacgt cctggcggac gacggagcag
    19141 cagggagatc tgacggtaag caacagcctg tttagacagg tcatcgtaaa tgatcagcgc
    19201 atcttcaccg cggtcacgga agtattcgcc cattgcgcaa ccggcatacg gtgccaggta
    19261 ttgcagtgca gcggattcag acgcggttgc taccacaacg atggtgttag ccagtgcgcc
    19321 gtgctcttcc agtttacgta ccacgttaga aatggtggac gctttctggc cgatagcgac
    19381 atagatacat ttgataccgg aatcgcgctg gttgatgatg gcatcgatag ccagtgcggt
    19441 tttacctgtc tgacggtcac cgatgatcaa ttcacgctga ccacgaccga ttgggatcat
    19501 ggagtcaacg gctttataac cggtctgtac cggctgatct acggactgac gttcgataac
    19561 gcccggagcg attgcttcta cagcagagaa gccgtcgtga tccagcggac ctttaccgtc
    19621 gattggtgca cccagagtgt taaccacacg gcccagcagg ccacggccaa ccggaacttc
    19681 caggatacgg ccagtacact taactttcat gccttcggca aggtcagcgt acggacccat
    19741 aacaaccgca cctacagagt cgcgctcaag gttcagtgcg atagcgtaac ggttacccgg
    19801 cagggagatc atttcaccct gcatacaatc ggccaggccg tgaatgcgga taacaccgtc
    19861 acttacagaa acaatagtac cttcgttgtg agcttcactc acaacattga actgagcaat
    19921 gcgctgcttg atcagttcgc tgatttcggt ggaattcagt tgcatgctcc agtcccctta
    19981 agactgcaag acgtctgcaa ggcgctcaag acgaccgcgt acgctgccat caatgaccat
    20041 atcacccgct cggatgataa cgcctgccat tacagactta tcgattttgc aattcagctt
    20101 aactttgcgt gacagacgtt tttccatcgc agcagaaatt ttcgcgagct gttgttcact
    20161 cagtgcggca gcggaaatga cgtctacctc agcggtagcc tcactcacgg cacgaaggtg
    20221 aataaactgc tccagaacat ccgggagcgc gttaagacga ccattttcag ccattacccg
    20281 aatcaggttc tgaccgtttt cgtccagttg ctcaccacaa actgcgataa acgactcggc
    20341 gagcgtttct ggcgcaagcg cgccagagag aagctctgcc atttgttcgt ttttggttac
    20401 ctcggcggca aacgccagca tgtcctgcca gcgttctaca ctttggtgtt cgacggcaaa
    20461 gtcaaaagct gctttggcgt aggggcgagc taccgtaata aattcagaca tcagcccctc
    20521 cctccttaca gttcagcgac aagtttatcc acgatgtcgc tgttagcagc ttcatccacg
    20581 gaacgttcga tgatcttctc ggcgccagca acagccagga tagcaacttg cttacgcagc
    20641 tcttcacggg cacgtttacg ctcggcttca atttctgcct gcgcctgggc cacgatttta
    20701 gtacgttcct gttctgcctc agctttcgct tcgtccagaa tctgcgagcg gcgtttgttc
    20761 gcctgctcga tgattacctg ggcttccgct ttcgcttttt tcagctggtc ggtcgcgctg
    20821 gcctttgcaa ggtcaaggtc cttatgtgct cgttctgcgg aagcaaggcc gtcagcaatt
    20881 tctttttgac gtttttcgat ggctgccatt aatggcggcc atacgtactt catgcagaac
    20941 agaacgaaca ggacaaacgc gatggcctgg ccgaggattg ttgcgttaag attcacagca
    21001 caatgcctct atttagttaa cgttctgata ttgctcttta aataaaagca acgcttacta
    21061 cgcgacagcg aacatcacgt acagacccag acctacagcg atcatcggga tagcatccac
    21121 cagacccata acgataaaga actgagtacg cagcagagga atcagatcag gttgacgcgc
    21181 tgcgccttcc aggaatttac ccccgaggat gccgataccg atcgcagcac cgattgccgc
    21241 cagacccatc atcacagcgg cagccatgta cagcagatcc atattcaggt tttccatgac
    21301 agtctccagt ttgtttcagt taaaacgtag tagtgttggt aaattaatgt tcttcagacg
    21361 ccatcgacag atagacgatc gtcagaacca tgaagatgaa ggcttgcagc gtaatgatca
    21421 ggatgtggaa aatggcccac ggcacattca ggatccactg tgaccaccac ggcaacagac
    21481 cagcaatcag aatgaaaatc agctcaccgg catacatgtt accgaacagt cgcaaaccga
    21541 gtgaaactgg tttggacagc aggcttaccc cttcaaggat taagttgaca ggaatgaacg
    21601 cccagtgatt gaacggctgc agcgtcaact ctttcgtgaa gccgccgatg cctttcattt
    21661 tgatgctgta gaacagaatc aggataaata cgcccagtgc catagacagc gttacgttca
    21721 cgtccgcaga cggaaccaca cgcagtgcag gcagacccag tacatgttca gcaatgtacg
    21781 gcagcaggtc gataggcagt aaatccatca ggttcatcag gaatacccag acgaagatcg
    21841 tcagggccag cggagcaatc agcttgcttt tgccatggta catgtctttc acgctaccat
    21901 taacaaagcc gatcaccagc tcaatcgcgg tctgaaactt acctggcaca ccgctggtcg
    21961 cctttttggc tacgctacgg aataaaacca ggaacaacag acccagcacc accgagaaga
    22021 acatggagtc aatattgatt gtccagaagg tggctggggg gttttgtgga tccaccagcg
    22081 agaatgtacg caggtccagc tgaaggttat tcaggtggtg tcctatgtaa tcctgcggcg
    22141 tcatattttc tgaagccatg atgcctttta ccctttgttg ttaattacag ccggtgccag
    22201 tatctgaacc accagcacca aaacccacgt aacgatcagc ggcaagaata ccgcctttaa
    22261 aaccgccaac gccaccacca gtaacaccaa catcgccaga actttgaaag cttcgccaaa
    22321 tgcgaatgtc caggccaccc ggcctttcgc tggtgtatac gcctggtgac gccaggcaaa
    22381 tatcataaac aaaacgttag gcagaaagac tgccaggccc ccgcttattg cagagacgcc
    22441 ccagaagggg tctttgaggc tgaacagcaa tccacttgct atcaccacca gtaactgaac
    22501 gagcagaagc ttccgagcaa cgtttcgact cacgagcgac acagacatca cgtttttcac
    22561 tcctgctccc ttcgaggtat gccgcgtgtc gtataaaact ttctttaagg cttagagtca
    22621 agcatcaaaa agcggtcaaa ttatacggtg cgcccccgtg atttcaaaca ataagtagcc
    22681 aaaaggtgaa taaatgttta aatatttttc cagtgcatac aattgcgact tttccgctaa
    22741 ccctgttcga tcatgaaaaa ctgtaaataa cgcgtaaaca ctagtgataa agcgtgcttc
    22801 agatcacata ttgcgcatgt tcgcgcacag tatatttatt tacttggcaa atgatgcctt
    22861 tgcaagttta tgatatttca gtc
//
LOCUS       DAEEKQ010000055        22731 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.055, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000055 DAEEKQ010000000
VERSION     DAEEKQ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.055"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..455)
                     /gene="folK"
                     /locus_tag="KLK57_002030"
     CDS             complement(<1..455)
                     /gene="folK"
                     /locus_tag="KLK57_002030"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HBE0892397.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRA"
     gene            complement(452..1870)
                     /gene="pcnB"
                     /locus_tag="KLK57_002031"
     CDS             complement(452..1870)
                     /gene="pcnB"
                     /locus_tag="KLK57_002031"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HBE0892398.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1909..2835)
                     /gene="gluQRS"
                     /locus_tag="KLK57_002032"
     CDS             complement(1909..2835)
                     /gene="gluQRS"
                     /locus_tag="KLK57_002032"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HBE0892399.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWTLTAVPESAIVNSTFSNASC"
     gene            complement(2872..3327)
                     /gene="dksA"
                     /locus_tag="KLK57_002033"
     CDS             complement(2872..3327)
                     /gene="dksA"
                     /locus_tag="KLK57_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HBE0892400.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3505..4209)
                     /gene="sfsA"
                     /locus_tag="KLK57_002034"
     CDS             complement(3505..4209)
                     /gene="sfsA"
                     /locus_tag="KLK57_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HBE0892401.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATRGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMNVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLSVTL"
     gene            complement(4224..4754)
                     /gene="thpR"
                     /locus_tag="KLK57_002035"
     CDS             complement(4224..4754)
                     /gene="thpR"
                     /locus_tag="KLK57_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HBE0892402.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4828..7257
                     /gene="hrpB"
                     /locus_tag="KLK57_002036"
     CDS             4828..7257
                     /gene="hrpB"
                     /locus_tag="KLK57_002036"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HBE0892403.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERHYLPLPAHQRFDEAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFEPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPVVNLMAAKRL
                     LQMLGALDGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPLMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANGMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGGVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7453..9987
                     /gene="mrcB"
                     /locus_tag="KLK57_002037"
     CDS             7453..9987
                     /gene="mrcB"
                     /locus_tag="KLK57_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HBE0892404.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDGELYE
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10207..12396
                     /gene="fhuA"
                     /locus_tag="KLK57_002038"
     CDS             10207..12396
                     /gene="fhuA"
                     /locus_tag="KLK57_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HBE0892405.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAAKHSATATKTDTPIEKTPQSISVVTNEEMQMRQFQSVKEA
                     LGYTPGVTVSSRGASNTYDFVIIRGFSSVGLNQNNYLDGLKLQGNFYNDAVIDPYMLE
                     RVELMRGPTSVLYGKSNPGGIISMVSKRPTTEPLKEIQFKMGTDNLFQTGFDFSDALD
                     DNGEFSYRLTGLARSTNEQQKNSESQRYTIAPSFSWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGASNNTYSRNQKMVGYSFEHGFNDTFTVRQNLR
                     FSEMKTSQKSVYGTGIAGDGHTLNRGTVVDNERLQNFSVDTQLESKFATGDVEHTLLT
                     GVDFMRMRNDINASFGSAPSIDLYNKYHPEYFAFGSAEPYQMNESKQTGIYVQDQAEW
                     NKWVFTLGGRYDWSKQATTVRENSYTPTEGYIERNDHQFTWRGGVNYLFDNGISPYFS
                     YSQSFEPSAFDLWSNPRISYKPSKGEQYEAGVKYVPNDMPVVVTGAVYQLTKTNNLTA
                     DPTNPLAQVPAGEIRARGVELEAKAALNANINLTASYTYTDAEYTKDTNLKGKTPEQV
                     PEHMASLWGDYTFNEGPLSGLTLGTGGRFIGSSYGDPANTFKVGSTAVMDAVVKYDLA
                     RFGMAGSSLAVNVNNLLDREYVASCFQTYGCFWGAERQVVATATFRF"
     gene            12447..13244
                     /gene="fhuC"
                     /locus_tag="KLK57_002039"
     CDS             12447..13244
                     /gene="fhuC"
                     /locus_tag="KLK57_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HBE0892406.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13244..14134
                     /gene="fhuD"
                     /locus_tag="KLK57_002040"
     CDS             13244..14134
                     /gene="fhuD"
                     /locus_tag="KLK57_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HBE0892407.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14131..16113
                     /gene="fhuB"
                     /locus_tag="KLK57_002041"
     CDS             14131..16113
                     /gene="fhuB"
                     /locus_tag="KLK57_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HBE0892408.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALVGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVAAERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSIGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLYRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16271..17551)
                     /gene="hemL"
                     /locus_tag="KLK57_002042"
     CDS             complement(16271..17551)
                     /gene="hemL"
                     /locus_tag="KLK57_002042"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HBE0892409.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIIEPVAGNMNCVPPLPD
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVEPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17776..19197
                     /gene="clcA"
                     /locus_tag="KLK57_002043"
     CDS             17776..19197
                     /gene="clcA"
                     /locus_tag="KLK57_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HBE0892410.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19221..19286
                     /locus_tag="KLK57_002044"
     CDS             19221..19286
                     /locus_tag="KLK57_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892411.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19279..19623
                     /gene="erpA"
                     /locus_tag="KLK57_002045"
     CDS             19279..19623
                     /gene="erpA"
                     /locus_tag="KLK57_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HBE0892412.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19670..20292)
                     /locus_tag="KLK57_002046"
                     /pseudo
     CDS             complement(19670..20292)
                     /locus_tag="KLK57_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
     gene            complement(20330..21130)
                     /gene="btuF"
                     /locus_tag="KLK57_002047"
     CDS             complement(20330..21130)
                     /gene="btuF"
                     /locus_tag="KLK57_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HBE0892413.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21123..21821)
                     /gene="mtnN"
                     /locus_tag="KLK57_002048"
     CDS             complement(21123..21821)
                     /gene="mtnN"
                     /locus_tag="KLK57_002048"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HBE0892414.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21905..>22731
                     /gene="dgt"
                     /locus_tag="KLK57_002049"
     CDS             21905..>22731
                     /gene="dgt"
                     /locus_tag="KLK57_002049"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HBE0892415.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEQLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVA"
BASE COUNT         5140 a         5793 c         6359 g         5439 t
ORIGIN      
        1 gctcttgtat gtaagacttc acgcaacgtc tccccatcag gaaacgccag ctccggtgca
       61 atctcaaaca gcggccacag cataaatcca cgattcttca tatcgtagtg cggaacggtc
      121 aggcgttcgg tatttatcac ttcattacca aacagcatga tatcgagatc cagcgtgcgt
      181 ggtccccagc gttcagcttt acgaacacga ccctgttgca gttctatgcg ctgtgtatgg
      241 ttgagtaact cttccggggc gagcgaagtt tcaagcgcca cggcggcgtt gaggtaatcg
      301 ggttgatctt gcggccccag cggtggagtg cggtaaaacg aagaaacggc aagaatgtgg
      361 ctttcaggga tatcgcctaa tgctttcagg gcagcattga cctgctccag cggagaggcc
      421 agattgctgc ctatggcaat atacgccact gtcatgcggt accctcacga cgcggtgcgc
      481 gtttgcgtgg acgacgagta cggcgacgcg gtgacggctc ttcatccaac tcgttaagca
      541 tccctttttg atctggtggc gcggaaacct ggaactcacc ccaccatttc accagacgct
      601 gcagttcagc gttacgttca acttcagctc gcaaggccaa caggtcataa gccgcacgga
      661 acttaggatg ctccagcagt ttccatgcgc gtttaccctg acgtcgggac atacgcaact
      721 gcaactgcca gatatcgcgg gttaacgtcg tcaggcgttt cgggattgcc agtgaacggc
      781 aggcttcgtc cagcacgtcg ttcatcgcca gcgcgaaagc gtcgtgatag gtcaggccgc
      841 tttcctgggc gatcttctgt gccgtctcca gcagtgggta ccagaacatg gcggcaaaca
      901 agaacgccgg gttcacgcgc atatcgttat ggatacgcgt atcggtattc ttcagcacct
      961 gttcaatgat ccgctccatc gggctgtcgc cattttccgt gaagtagcgg gtaatggtcg
     1021 ggaacagcgg ctggaacaga tgatattcac acaacagctt ataggtttcg taaccgtagc
     1081 ccgcttgtag cagtttaagc gattcttcaa acaggcgtgc cggtgggata tcgttcagca
     1141 gggtagcgag gcgagggatc ggttctgcgg tttccgggct gatgcgcata cccaatttgg
     1201 cggcaaaacg taccgcgcgc agcatccgta ccggatcttc acggtagcgc gtttccgggt
     1261 taccaatcag acggataacg ccgtccttca gatccttcat accgccaacg taatcacgga
     1321 cggtaaaatc cgctacgctg taatagaggc tgttgatagt gaaatcgcgg cgctgggcgt
     1381 cttcttcgat ggagccgaaa atgttgtcgc gcagcaacat gccgttttgc ccgcgttggg
     1441 aggtcgtgcg gtcgctgacg ttaccttcgt ggtgtccacg gaaggtcgca acttcgataa
     1501 tctccgggcc aaacattaca tgagccagac ggaaacggcg acccaccagg cggcagttac
     1561 ggaacagttt gcgcacctgc tcaggcgtgg cgttagtggt tacgtcaaaa tctttcggct
     1621 ttttgccaag taacaggtcg cgcacgccgc cgccaaccag ccaggcttcg tatcccgctt
     1681 tgttgagcct gtacattacc ttcagggcat tttcactgat atctttgcgg gaaatagcat
     1741 gctgctcacg cgggatcacc gtcacctgtg gacgggcgac tgcctgttca gcctcgcttt
     1801 cctcgcggct tagcaccttg cggcaaaaat tagcgactcg ggtaaaaata gtacacctcg
     1861 gtagtgtcaa acatcattca ggacaaaaaa atagcggcta atcatagctc agcatgacgc
     1921 atttgagaat gttgaattta caattgccga ctcgggcacg gcggtcagcg tccagttttt
     1981 gactgctgac tgaaggattt gttcgacgct gaaatcctgc cagtgtgctt ctgcctgctg
     2041 acccagaaat tgaagtgccg cgattagtac cggacgtgga tcgcctttcg gcaacgcagg
     2101 cgcatgattc tgcttggaaa gtttagcgcc ttgtggatta agcgccagcg gcagatgaat
     2161 gtaatctggc actttccagc caaaaagctg gtacagcgag atttgcctta ctgttggttc
     2221 aatcagatca gccccacgca ctatttctgt aacgccctgg aaatgatcat caaccacaac
     2281 agccaggttg taggcgaaca acccatcacg gcgatgaatg ataaaatctt cccgtgccag
     2341 tttttcgtcg gcgtgaataa tgccgcgcag ctggtcagta aattgcgtga ccggatgctg
     2401 ctggcggata cgcactgcgg cattgtctgg tccatgatgc aacacccggc aatgaccgtc
     2461 gtaaataccg ccaatgcttt gaatacgcgc acgcgtacag gtgcagtaat aacttagtcc
     2521 ttgttcatgt aaccaggcga gtgcttcacg ataggcgtcg tgacgttgcg attgccagag
     2581 aacatcgccg tcccagtgca gaccgtaatg ttccagctgg cgcaggatag tttctgcggc
     2641 accgggaact tcacgaggcg ggtcgatatc ttctatgcgt accagccagc gaccttgccg
     2701 ggcgcgagcc tgcaaatagc tgccgagcgc ggcgatcaga gagccaaaat gaagctcgcc
     2761 ggaaggagag ggggcgaagc ggccaatata ctgtgtgtct gtcatctctt tgaacaaaaa
     2821 ataaggcggg agcatttccc gcctgtggta aacgtgatgg aacggctgta attagccagc
     2881 catctgtttt tcgcgaattt cagccagcgt tttgcagtcg atgcacagat cggctgtcgg
     2941 gcgcgcttcc agacggcgaa taccaatttc aacaccgcag gattcgcagt agccgaaatc
     3001 ttcgtcttcc acttttttca gcgtcttctc gatctttttg atcagcttac gctcgcgatc
     3061 gcggttacgc agttcgaggc tgaactcttc ttcctgggct gcacggtcta ccgggtccgg
     3121 gaagttggct gcttcatcct gcatatgtgt aacggtgcga tcgacttcat ccctgagttg
     3181 attacgccat gcttccagaa tacgacggaa gtgcgccagc tgggcttcat tcatatactc
     3241 ttcgcccggc ttctcctgat atggttccac cccagcgatg gcgagaatac tcagggacga
     3301 tgttttacgg ttttgccctt cttgcatgtt gcttctcctt aacacgcact atcgatcccc
     3361 atgttcgggg gaaaaatgag gccgctataa atagcagatg cttttccgga tagcaattat
     3421 ctaaacgtaa cacttgacaa ctgtgtgagg aaaagcgtat ttgcgcacgc gaccagaatg
     3481 taaattaacc agttacttac tttactacaa tgtaaccgac agtgattttt taagagccat
     3541 gccttcagca gaaatttccg ctttgtaagc cagaatttct acccccctct gttgagcttc
     3601 tgacaatagt tgcgcgtatt tctcatcgat gtggcgcgcg ggtgaaaacc gtgtaatggc
     3661 tgaatgcagc acggcgaaaa agataaccgc acgctggcct tcagccgcta cgttcatcaa
     3721 ctcccgaagg tgtttctgac ctcgttcagt gaccgcatcg ggaaaatatc cctgttcgtt
     3781 ctccgctaac gtaaccgatt tcacttcaat atagcagtct ggacgcgaat ccgcctgcaa
     3841 cataaagtca atacggctgc gttctgcgcc gtattttact tcgcttttca gcgagctata
     3901 gcctgacagt tctgaaattg attcattaag gatagcctct ttcgtcaacc tgttagccca
     3961 aagcgtattg acgcaaataa atgcgccgct ctggctttga gttagttccc aggtgtgtgg
     4021 gtatttccgt ttggtgttgt ctgaagtcga ataccagacg gtatcgccac gcgttgcaca
     4081 accggtcatc gcacccgtat tcgggcagtg tagcgttaat tcgcgaccat cgggtgtgat
     4141 cacatcggct aaaaaacgtt tgtaacgctg aattagcgtc gcgcgctgta gagggggaga
     4201 gaattccatt gcgacaatcc ttgttattgc gttagcgccc agcgttttag cggcgtgtag
     4261 cgtgtgcgtc cacgggcaaa cgaggaggcg taaagggtga actccgtcac cgcataagac
     4321 cagttaaaac ctggcggcgg gattgtcacc gcctcgctgg cgtcgcgcaa taaggtaata
     4381 tgtggatgaa acggacgatt gctttgaaaa caaccgctgc gggcagcctg tgaacggagc
     4441 atattcgcca gctggattaa gccgcgtggc ggctgacgca tccctaacca caccacacgc
     4501 gaacgcagcc attgtccggc gtcatcaagc gtgagtgtga aaccaggttg acgaatccgt
     4561 ccggctaaaa gagaaagcgc cttctctttc tcggcgctca cttcgcctaa aaatgccaga
     4621 gtcagatgca aattatcggc ggcgaccgga cgtcccgcct caggtgggaa gtgtgtggcg
     4681 cgccagtgga taatctgttc gcggatttct gcaggtaagt cgatagcaaa gaacagacgt
     4741 tgcggttcag acatgtgagg cactcggtta tgaattaccg cgatgctaca atgtggcgcg
     4801 aagaatgtta accctctgga gcgttttgtg tcgtcgttgc ccgttgctgc cgtcttacct
     4861 gaattactta ccgctctcga ttgtgcgccg caggtattat taagtgcgcc gaccggggcc
     4921 gggaaatcaa cctggctgcc gctgcaactg ctggcgcatc ccggcattaa cgggaaaatt
     4981 atcctgctgg agccgcgtcg tctggcggca cgtaacgtcg cgcaacggct ggcggagctg
     5041 cttaacgaaa agccaggcga taccgttggc taccggatgc gtgcgcaaaa ctgcgtcggg
     5101 ccaaataccc gtctggaagt agttaccgaa ggcgtgctga cgcggatgat ccagcgtgac
     5161 ccggagttga gcggtgttgg attggtgatc cttgatgaat ttcatgagcg cagcttgcag
     5221 gcggatttgg cgttggcgct gttactcgat gtgcaacaag gtctgcgtga tgaccttaaa
     5281 ctgctgatta tgtcggctac gctggataac gaccgcttgc agcaaatgct gccagaagcg
     5341 cccgtcgtca tctcagaagg gcgctcgttt ccggttgaac gccattattt accgctgccc
     5401 gcgcatcagc gttttgacga agccgttgcg gtagccaccg ccgaaatgct gcgtcaggaa
     5461 agcggatcat tactgttatt tttacctggc gtcggagaaa ttcagcgtgt gcaggaacaa
     5521 ctggcttcgc gcatcggcag tgatgtgttg ctctgcccgc tgtatggcgc gttgtcgctg
     5581 aacgatcagc gaaaagcgat cctcccggca ccgcaaggga tgcgcaaagt ggtgctggcg
     5641 accaatattg ctgaaaccag tttaaccatt gaaggtattc gtctggtggt ggattgtgcc
     5701 caggagcgtg tggcgcgttt tgagccgcgc acggggctta cgcgactgat tactcaacgc
     5761 gttagccagg catccatgac gcagcgtgcc gggcgcgccg ggcgtctgga gccgggtatc
     5821 tgcctgcatt taatcgccaa agaacaagca gaacgcgctg ccgcgcaaag tgaaccggag
     5881 atcttacaaa gcgatctttc cggtttgctg atggaattac tgcaatgggg atgcagcgat
     5941 ccggcgcaga tgagctggct ggatcaaccg ccagtagtga atctaatggc cgcgaaacgt
     6001 ctgttacaaa tgctgggggc gctggacggt gaacggctta gtgcgcaagg gcaaaaaatg
     6061 gcagcgctgg gtaacgatcc gcgtttagcg gcaatgctgg ttagcgcgaa gaacgacgac
     6121 gaagctgcta ccgcggcaaa aattgccgcc attctcgaag agccgccact gatgggcaat
     6181 agtgacctgg gcgtggcgtt ttcgcgcaat caaccagcct ggcagcaacg tagtcagcaa
     6241 ctgttaaaac gcttaaacgt acgcggcggt gaggcagaca gttcgcttat cgcgccgcta
     6301 cttgccgggg cgtttgccga tcgcattgct catcgccgtg ggcaagatgg acgctatcaa
     6361 ctggcaaacg gcatgggagc gatgctcgat gccgacgacg cgctaagccg ccacgaatgg
     6421 ttgatcgcac cgttattatt gcagggcagc gcctcgccgg atgcgcggat tttactggcg
     6481 ctaccggtcg atattgatga attagtacaa cgctgtccgc agctggtgca gcaatctgac
     6541 accgtggaat gggatgacgc gcaaggtacg ctgaaagcct ggcgtcggct acaaatcggt
     6601 cagttgacgg tgaaagtgca gccgctggcg aaaccgtcag aagacgagtt gcatcaggcg
     6661 atgcttaatg gcatccgtga taaaggttta agcgtgctca actggacggc ggaagcggaa
     6721 cagctacgct tgcgtttgtt atgcgccgca aagtggttgc cggaatatga ctggccagcg
     6781 gttgatgatg aaagtttatt ggcagcgctg gaaacgtggc tgctgccaca tatgacaggc
     6841 gtacattcac tacgcggcct gaaatcactg gacatttatc aggcactacg cggattactt
     6901 gattggggaa tgcagcaacg tctggatagt gaattgcctg cgcattacac tgtgccgacg
     6961 ggaagccgga tcgccattcg ttatcatgaa gataacccgc ccgcactggc ggtgagaatg
     7021 caggagatgt ttggtgaggc cactaatccg actatcgccc aggggggcgt gccgctggtg
     7081 ctggagttgc tttcacccgc ccaaaggcca ctacaaatca cacgagattt gagcgccttc
     7141 tggaaaggag cgtaccgtga ggtgcaaaaa gagatgaaag ggcgttatcc caaacacgtc
     7201 tggccggacg acccggcaaa cacggcaccg acgcgacgga cgaaaaagta ttcgtaaggt
     7261 tggttttctc cctctccctg tgggagaggg ccggggtgag ggcatcagcg cgcacgttca
     7321 tcctcaccct aaccctctcc ctcaagggag aggggaccga tcgagcacaa attttgagag
     7381 atatcttctt ctgtcttgta acagaagaac agaaaatcgg gcttttgcgc ctgaatattg
     7441 cggagaaaaa gcatggccgg gaatgaccgc gagccaattg gacgcaaagg gaaaccgacg
     7501 cgtccggtca aacaaaaggt aagccgtcgt cgttacgaag atgacgatga ttacgacgat
     7561 tatgatgact atgaggatga agaaccgatg ccgcgcaaag gtaagggcaa aggcaaaggg
     7621 cgtaagcctc gtggcaaacg cggctggcta tggctgctgt taaaactggc tatcgttttt
     7681 gccgtgctga tcgccattta cggcgtttat ctcgatcaaa aaattcgtag ccgtattgat
     7741 ggcaaggtct ggcaactgcc cgcggcagtt tatggccgaa tggtcaacct tgaaccagat
     7801 atgaccatca gcaagaacga aatggtgaag ctgctggagg caacacagta tcgtcaggtg
     7861 tcgaaaatga cccgtcctgg cgaatttacc gtgcaggcca acagcattga gatgatccgc
     7921 cgtccgtttg atttcccgga cagcaaagaa gggcaggtgc gtgcgcgtct gacctttgat
     7981 ggcgatcatc tggcgacgat cgtcaatatg gagaacaacc gtcagttcgg tttcttccgt
     8041 cttgatccgc gcctgatcac catgatctct tcgccaaacg gtgagcagcg tctgtttgtg
     8101 ccgcgcagtg gtttcccgga cttgctggtg gatactttgc tggcgacaga agaccgtcat
     8161 ttctacgagc atgatgggat cagtctctac tccatcggac gtgcggtgct ggcaaacctg
     8221 accgccggac gcacggtgca gggagcgagt acgctgacgc aacagctggt gaaaaacctg
     8281 ttcctctcca gcgagcgttc ttactggcgt aaagcgaacg aagcttacat ggcgctgatc
     8341 atggacgcgc gttacagcaa agaccgtatt cttgagctgt atatgaacga ggtgtatctc
     8401 ggtcagagcg gcgacaacga aatccgcggc ttcccgcttg cgagcttgta ttactttggt
     8461 cgcccggtag aagagctcag cctcgaccag caggcgctgt tagtcggtat ggtgaaaggg
     8521 gcgtccatct acaacccgtg gcgtaacccg aaactggcgc tggagcgacg taatctggtg
     8581 ctgcgtttgc tacaacaaca gcagattatc gatggtgaac tgtatgaaat gctcagcgcg
     8641 cgtcctctgg gcgtacagcc gcgcggtggg gtaatctctc cacagccagc ctttatgcaa
     8701 ctggtgcgtc aggagctgca ggcgaaatta ggcgataagg taaaagatct ctccggcgtg
     8761 aagatcttca ctacctttga ctcggtggcc caggacgcgg cagaaaaagc cgccgtggaa
     8821 ggcattccgg cactgaagaa acagcgtaag ttgagcgatc ttgaaactgc aattgtggtc
     8881 gtcgaccgct ttagtggtga agttcgtgcg atggtgggag gttctgagcc gcagtttgcg
     8941 ggctacaacc gtgcgatgca ggcgcgtcgt tcgattggtt cccttgcaaa accagcgact
     9001 tatctgacgg ctttaagcca gccgaaaatc tatcgcctga acacgtggat tgccgatgcg
     9061 ccaatcgcgc tgcgtcagcc gaatggccag gtctggtcac cgcagaacga tgaccgtcgt
     9121 tatagcgaaa gcggcagagt gatgctggtg gatgcgttga cccgttcgat gaacgtgccg
     9181 acggtaaatc tggggatggc gctggggctg cctgcagtta cggagacctg gatcaaactg
     9241 ggcgtgccga aagatcagct gcatccggtt ccggcgatgc tgctgggggc gttgaactta
     9301 acgccaatcg aagtggcgca ggcattccag accatcgcca gcggcggtaa ccgtgcaccg
     9361 ctttctgcac tgcgttcggt aatcgcggaa gatggcaagg tgctgtatca gagcttcccg
     9421 caggcagaac gcgctgttcc ggcgcaggcg gcgtatctga cactatggac catgcagcag
     9481 gtggtacaac gtggtacggg tcgtcaactt ggggcgaaat acccgaacct gcatctggca
     9541 gggaaaacag ggactaccaa caataacgta gatacctggt ttgcgggcat tgacggcagc
     9601 acggtgacca tcacctgggt aggccgtgat aacaaccagc cgaccaaact gtatggtgcc
     9661 agcggggcaa tgtctattta tcagcgttat ctggctaacc agacgccaac gccgctgaat
     9721 cttgttccgc cagaagatat tgcagatatg ggcgtggact acgacggcaa ctttgtttgc
     9781 agcggtggca tgcgtgtctt gccggtctgg accagcgatc cgcaatcgct gtgccagcag
     9841 agcgagatgc agcagcagcc gtcaggcaat ccgtttgatc agtcttctca gccgcagcaa
     9901 cagccgcaac agcaacctgc tcagcaagag cagaaagaca gcgacggtgt agccggttgg
     9961 atcaaggata tgtttggtag taattaacat ctaagcgtga aataccggat ggcgaattgc
    10021 catccggtaa aataacatcc cacctaaagt attaaccctt tcttttcatc tggttgttta
    10081 ttaacccttc aggaacgctc agattgcgta ccgcttgcga acccgccagc gtttcgaata
    10141 ttatcttatc tttataataa tcattctcgt ttacgttatc attcacttta catcagagat
    10201 ataccaatgg cgcgttccaa aactgctcag ccaaaacact cactgcgtaa aatcgcagtt
    10261 gtagtagcca cagcggttag cggcatgtct gtttatgcac aggcagcggt tgaaccgaaa
    10321 gaagacacta tcaccgttac cgctgcacct gcgccgcaag aaagcgcatg ggggcctgct
    10381 gcaactattg cggcgaagca ctctgctact gcgactaaaa cggatacacc aattgaaaaa
    10441 acgccgcagt ctatttcggt tgtgacaaat gaagagatgc agatgcgtca atttcagtct
    10501 gtaaaagaag cattaggtta tacacctggc gttacggtta gcagtcgtgg tgcttctaat
    10561 acatatgatt ttgttatcat tcgtggtttc tcatctgttg gtctgaatca aaataattac
    10621 cttgatggac taaaacttca gggcaacttt tataacgatg ctgtgattga tccttacatg
    10681 ctcgagaggg ttgaactgat gcgtgggccg acgtctgttc tctatggaaa aagtaatcct
    10741 ggtgggatta tttctatggt gagtaagcgt ccgaccactg aacccctgaa agaaattcag
    10801 tttaaaatgg ggacggataa tctgtttcag accggattcg atttcagtga tgcactggat
    10861 gataacggtg agttctctta ccgtttgacg ggcctcgctc gctcaacaaa cgaacagcag
    10921 aaaaactctg aatctcagcg ttataccatt gctccatcat tctcatggcg tccagacgac
    10981 aaaaccaatt ttactttcct gtcctatttc cagaatgaac cagaaacggg ttattacggt
    11041 tggttgccga aagaggggac cgttgagcca ttgcctaatg gtaagcgtct accgactgac
    11101 ttcaatgaag gtgcatcgaa taatacatac tcccgtaacc agaaaatggt gggatatagt
    11161 tttgaacatg gttttaatga caccttcacc gtgcgtcaga atctgcgttt cagtgaaatg
    11221 aaaacctcac agaaaagtgt ttatggcaca gggattgctg gtgatggtca taccctaaac
    11281 cgcgggacag tggtggataa tgagcgtcta caaaacttta gcgttgatac ccaacttgaa
    11341 agtaagtttg ctacaggtga cgtcgagcat actttgctga caggggtaga cttcatgcgg
    11401 atgcgtaatg atatcaatgc cagctttgga tccgcaccat ccatcgatct ttataacaaa
    11461 tatcaccctg aatactttgc atttggtagc gcagagccat accagatgaa tgaaagcaaa
    11521 caaacaggta tttatgttca ggatcaggcg gaatggaata aatgggtatt cactctgggg
    11581 ggacgctacg attggtctaa gcaagcgacc actgttcgtg aaaactctta tacgccgact
    11641 gaaggttata ttgagcgcaa tgatcatcag ttcacctggc gcggtggtgt aaattactta
    11701 ttcgataatg gtatttcacc ttactttagc tatagccagt cctttgaacc gagtgctttc
    11761 gatctgtgga gcaacccgcg catttcctat aagccatcga aaggtgaaca gtatgaagct
    11821 ggcgtaaaat atgttccgaa tgatatgccg gtcgttgtta cgggcgcagt ctatcaattg
    11881 acgaaaacaa ataacctgac agcagaccca acaaacccat tagcgcaagt cccagcaggt
    11941 gagattcgcg ctcgtggtgt ggaacttgaa gcaaaagcgg cgttaaatgc caatattaac
    12001 ttgacggctt cttataccta caccgatgcg gaatacacca aagacaccaa tctcaaaggt
    12061 aaaactccag aacaagtacc ggagcatatg gcatctctct ggggggatta taccttcaat
    12121 gaagggccgc tttctggttt aacattggga acaggtggtc gttttattgg ttccagctat
    12181 ggtgatccgg caaacacttt taaagtgggt agcacagctg taatggatgc tgttgtaaaa
    12241 tatgatctgg cacgctttgg tatggcggga tccagccttg ctgtgaacgt caacaatttg
    12301 ctcgatcgtg agtatgttgc cagttgcttc cagacttatg gctgcttctg gggcgcagaa
    12361 cgtcaggtcg ttgcaaccgc aaccttccgt ttctaatttc tcttttgggg cacggatttc
    12421 cgtgcccatt tcacaagttg gctgttatgc aggaatacac gaatcattcc gataccactt
    12481 ttgcactgcg taatatctcc tttcgtgtgc ccgggcgcac gcttttgcat ccgctgtcgt
    12541 taacctttcc tgccgggaaa gtgaccggtc tgattggtca caacggttct ggtaaatcca
    12601 ctctgctcaa aatgcttggt cgtcatcagc ctccgtcaga aggggagatt cttcttgatg
    12661 cccagccgct ggaaagctgg agcagcaaag cgtttgcccg caaagtggct tatttgccgc
    12721 agcagcttcc tccggcagaa gggatgaccg tgcgtgaact ggtggcgatt ggtcgttacc
    12781 cgtggcatgg cgcgctgggg cgctttggtg cggcagatcg cgaaaaggtc gaggaagcta
    12841 tctcgctggt tggcttaaaa ccgctggcgc atcggctggt cgatagtctc tctggcggcg
    12901 aacgtcagcg ggcgtggatc gccatgctgg tggcgcagga tagccgttgt ctgttgctcg
    12961 acgaaccgac ttcggcgctg gatatcgccc accaggttga tgtgctggcg ctggtgcacc
    13021 gtttaagtca ggaacgtggc ctgacggtca ttgccgtgtt gcacgatatc aatatggcgg
    13081 cacgctactg tgattatctg gtcgccctgc gcagtggtga aatgattgct cagggaacgc
    13141 ctgcggaaat tatgcgcggc gaaaccctcg aaatgattta tggcatcccg atgggtattt
    13201 tgccgcatcc ggcgggtgct gcacctgtga gttttgttta ttgatgagcg gcttacctct
    13261 tatttcgcgc cgtcgactgt taacggcgat ggcgctttct ccgttgttat ggcagatgaa
    13321 taccgcccac gcggcggcta ttgaccccaa tcgtattgtg gcgctggagt ggttgccggt
    13381 ggaattactg ctggcgctcg gcatcgtgcc ttacggcgtg gcggatacca tcaactatcg
    13441 cctgtgggtc agcgaaccac cattgccgga ctcagtgatt gacgtcggtt tgcgtacaga
    13501 acctaacctt gaactgctga ccgaaatgaa accgtcgttt atggtctggt cggcaggata
    13561 tggcccttca tcagaaatgc tggcacgtat tgcgccgggt cgcggattta acttcagtga
    13621 cggcaaacag ccgttggcga tggcgcgtaa atcgctgacg gaaatggcag atttacttaa
    13681 cctgcaaagc gcagcggaaa cgcatttagc gcaatacgaa gactttatcc gcagcatgaa
    13741 accccgcttt gtgaagcgtg gtgcacgccc gttactgctg acgacgctta tcgatccgcg
    13801 ccatatgctg gtcttcggtc caaacagctt gttccaggaa attctcgatg agtacggcat
    13861 cccaaatgcc tggcaagggg aaaccaactt ctggggcagt accgccgtca gtatcgatcg
    13921 tctggcagcg tataaagacg ttgatgtgct ctgttttgat cacgacaaca gcaaagacat
    13981 ggatgcgcta atggcaaccc cgctgtggca ggccatgccg tttgtccgcg ccggacgctt
    14041 tcagcgcgta cctgcagtct ggttttatgg tgcgacactc tcggcaatgc actttgtgcg
    14101 cgttctggat aacgccatcg gaggtaaagc gtgagtaaac gaattgcgct ttttccggcg
    14161 ttattgctgg cgctgttagt gattgtcgct acggcgctca cctggatgaa cttctcgcag
    14221 gcgctgccgc gtagccagtg ggcgcaggct gcctggtcgc cagacatcga tgtcatcgag
    14281 cagatgattt ttcactacag cttgttgccg cgtctggcga tttcgctgct ggtgggcgcg
    14341 ggcctggggc tggtgggcgt gctgtttcag caagtgctgc gtaacccgct ggcggagccg
    14401 acgacgcttg gcgttgctac aggcgcgcaa ctgggaatta ccgtcaccac actttgggcg
    14461 atcccaggtg ctatggcgag ccagtttgcc gcactggtgg gcgcttgtgt cgttggctta
    14521 atcgtctttg gcgtcgcgtg ggggaaacgg ctttcgccgg ttacgcttat tctcgcgggg
    14581 ctggtggtga gcctttattg cggggcaatt aatcagctac tggttatctt ccatcacgac
    14641 cagctgcaaa gcatgttcct gtggagcacc ggaacgctga cgcaaaccga ctggggcggc
    14701 gttgagcgtt tatggccgca gttgctgggc ggcgtgatgt tgacgttatt gctacttcgc
    14761 ccgttaaccc tgatggggct tgatgatggc gtggcgcgca atctcgggct ggccttgtcg
    14821 ctcgcgcgtc tggcggcgct gtcgctggcg atagtcatca gtgcgctgct ggtgaatgcg
    14881 gtggggatta tcggctttat cggtttgttc gcgccgctgc tggcgaaaat gctgggggcg
    14941 cggcgtttgt tgccacggtt gatgctggcg tcgctgattg gggcgctgat cctctggctt
    15001 tccgatcaaa tcatcctctg gctgactcgc gtgtggatgg aagtgtccac cggttcggtc
    15061 actgcgttga tcggtgcgcc gctgctactg tggctgttgc cgcgtttacg cagcattagc
    15121 gcgccggata tgaaggtcaa cgatcgtgtc gcggctgaac gccaacatgt gctggcgttt
    15181 gccctcgcgg gcggcgtgct gctgttgatg gctgtggtgg tggcgctgtc gtttggtcgt
    15241 gatgcgcacg gctggacgtg ggcgagcggg gcgttgctcg aggatttgat gccctggcgc
    15301 tggccgcgaa ttatggcggc gctgtttgcg ggcgtcatgc tggcggtggc gggctgtatt
    15361 attcagcggc tgaccggaaa cccgatggca agcccggaag tgctggggat tagctctggc
    15421 gcggcgtttg gcgtggtgtt aatgctgttt ctggtgccgg gtaatgcctt tggctggctg
    15481 ttacccgcgg gcagtatcgg ggcggcggtg acgctgttga tcattatgat cgccgccggc
    15541 cgcggtggat tttccccaca ccgtatgtta ctggcgggga tggcgttaag taccgcattc
    15601 accatgcttt tgatgatgtt gcaggcgagc ggtgacccgc gaatggcgca agtgctgacc
    15661 tggatttccg gttcgaccta caacgcgacc gatgcgcagg tctggcgtac cggaattgtg
    15721 atggtgattt tgctggcgat taccccgctg taccgccgct ggctgaccat tttaccgctg
    15781 ggtggtgata ccgcacgagc cgtaggaatg gcgctgacgc cgacgcgaat tgcgctgctg
    15841 ctgttagcgg cttgcctgac ggcgaccgcg acgatgacta ttggaccgtt gagttttgtt
    15901 ggtttaatgg caccgcatat tgcgcggatg atgggctttc gacggacgat gccacacatc
    15961 gtaatttcgg cgctggtggg tggtttactg ctggtattcg ctgactggtg tgggcggatg
    16021 gtgctgtttc cattccagat cccggcgggg ctgctgtcga cctttatcgg cgcgccatat
    16081 tttatctatt tgctgagaaa gcagagccgt taatttttcc atataacggc cctgtacgcc
    16141 tggacggata aagcgtttac gcgacaacgg atgcccgatg cgacgctggc gcgtcttatc
    16201 gggcctacaa acggccccga atcgtaggtc ggataaggcg ttcacgccgc atccgacaaa
    16261 ccatgctgga tcacaacttc gcaaacaccc gacgtgcagc atcgatggtg ttattgatat
    16321 cttccatgct gtgcgccacg gacataaagc ccgcttcaaa cgctgacggt gccaggtaaa
    16381 caccttcgtc cagcatcata tggaagaaac gcttaaagcg ttccacgtca caggccatca
    16441 catcctgata gcacgtcacg gactcggcgt cggtaaagaa aataccgaac atgccgccaa
    16501 cgtggttaac gaccagcgga attccggctt cttctgccgc ttccagcaga ccttctgcca
    16561 gacgtgttgt cagctcatcc aacgtttcgt gaacgcccgg ttgcgcgact tcattcagac
    16621 aggcgaaacc ggctgccatc gcaattgggt taccggaaag cgtacccgcc tgatagaccg
    16681 gacccgtcgg ggccagcgca tccattacat cacgacgacc accgaatgcg cctaccggca
    16741 ttccaccgcc gatgattttg cccaggcagg tgagatccgg ttccacaccg taataatcct
    16801 gtgcgccagc cagtgccacg cggaagccgg tcattacttc atcgatgatc agcaatgcgc
    16861 caaattcgtc gcacagcgca cgcagacctg gcaggaagtc tggcagcggt ggaacacagt
    16921 tcatattgcc tgccaccggc tcgatgataa tacaggcaat ctcttgcggg tattgctcaa
    16981 acgcggcgcg tacagaagcc agatcgttat aagtacaggt taaggtatgt ttggcgaaat
    17041 ctgccggaac gcccggcgag tttggctggc ctaacgtgag tgcgccagaa ccggctttca
    17101 ccagcaggca gtcagcgtga ccgtggtaac acccttcaaa tttaatgatt ttgtcgcgac
    17161 cggtaaaacc acgggccaga cggatggcgc tcatcgtcgc ctcggtgccg gagttcacca
    17221 tgcgcaccat atccatggtc gggaccaatt cggtcaccag ttgcgccatt ttcacttcca
    17281 tttcggttgg tgcaccaaag cttaaaccac gctcggcggc ttcaatcacg gcattgcgga
    17341 ttgccggatg gttatggccc agcaccatcg gcccccagga accgacataa tcgatatagg
    17401 ctttgccatc aacatcgtac agataagcgc cgtccgcttt ttcgataaac agtggagtgc
    17461 cgcccacgcc agtaaaggcg cgaacagggg agttcacacc gccagggatc agctcgcgcg
    17521 ctgcgctgta aagattttca gacttactca tggagggttc ctgattcgta gaaaaagtga
    17581 atggctgcta ttctatgtta ttcataacaa gttaaatact cgtcaaacat caggctgctt
    17641 gtactggtgc aatcctgatt tcttagagta taaaagtttt gtgcatttga aacattacgc
    17701 tttgcaaagg attttcatgg aacgtgcgag taaaatgccg tcatcttatt tgtatgacca
    17761 ataagtgatc attggatgaa aactgatact ccctctttag aaacaccgca ggccgcgcgc
    17821 ctgcgacgca gacaactgat tcgccaactt cttgagcgcg ataaaacccc gttagccatt
    17881 ttgtttatgg cggcagtcgt cggcacgctt gttgggctgg cagcggttgc ttttgacaaa
    17941 ggtgtcgcct ggttgcagaa ccaacgtatg ggggcgctgg tacatactgc tgataattat
    18001 ccgcttctgt taaccgtcgc ttttctctgt tcggcggtgc tggcgatgtt tggctacttt
    18061 ttggtgcgca aatacgcgcc ggaagcaggt ggttcgggga tcccggaaat tgaaggggcg
    18121 ctggaagatc aacgtcccgt tcgctggtgg cgtgtattgc cggtgaagtt ctttggcggg
    18181 ctggggacac tcggcggagg catggtgttg gggcgcgaag ggccaaccgt gcagatcggc
    18241 ggtaacattg gccgtatggt gcttgatatt ttccgcctga aaggtgacga agctcgccat
    18301 acgctgctgg caaccggtgc tgctgcgggg ctggctgcgg cctttaacgc gccgctggcg
    18361 ggtattttgt ttattatcga agagatgcgt ccgcagtttc gctatacgtt aatttcgata
    18421 aaagcggtat ttattggcgt cattatgtcg accattatgt accggatttt taatcatgaa
    18481 gttgcgttga ttgacgtcgg taaactttct gatgcgccgc ttaatacgct gtggctttat
    18541 ctgatcctcg gtattatttt tggcattttc ggccctattt ttaataaatg ggtgctgggg
    18601 atgcaggatt tgctgcaccg tgtgcacggc ggcaatatta ccaaatgggt actgatgggc
    18661 ggtgcgattg gcggtctgtg tggattgctg gggtttgtgg caccagcaac gtcgggcggc
    18721 ggttttaacc tgattcctat cgctaccgcg gggaatttca gcatgggaat gctggtgttt
    18781 atcttcgtcg ctcgggtcat taccacctta ctctgcttct cttccggcgc gccgggcggt
    18841 atttttgccc cgatgctggc gctgggtact gtgctgggaa ccgctttcgg aatggttgcc
    18901 gttgagctgt ttccgcaata tcaccttgag gcggggacgt ttgctattgc cggaatgggg
    18961 gcattactgg cggcatctat tcgcgcgccg ttaacgggga tcattctggt tctggagatg
    19021 accgataact accagctcat tttgccaatg attattaccg gtcttggcgc aacactatta
    19081 gcgcaattta ccggcgggaa accgctatac tcggcgattc ttgcgcgcac gctggcaaaa
    19141 caggaagctg agcaactggc gcgaagcaag gccgcatcag ccagcgagaa tacttgaacg
    19201 aaataccagg gtattagata atggcgatta ttattgggtt agaatttgcc caattgccga
    19261 tgtcgtttgg agcaaaatat gagtgatgac gtagcactgc cgctggagtt taccgacgca
    19321 gcagccaaca aagttaaaag cctgatcgct gacgaagata acccgaatct gaaattacgc
    19381 gtgtatatca ccggtggcgg ttgcagcggc ttccagtatg gtttcacctt tgatgatcag
    19441 gtgaacgaag gcgatatgac catcgaaaaa cagggcgttg gcttggtggt tgatccgatg
    19501 agcctgcaat atctggtcgg cggttccgtt gactataccg aaggactgga aggttctcgt
    19561 ttcatcgtga ccaacccgaa cgcgaaaagc acctgcggtt gcggttcttc ctttagcatc
    19621 taatctgttg ttcatgtgcc ggataaacta tccggcgcat cttctctctt caacgcccat
    19681 tctcatccag cgcaaacgtc ggtagcttaa gatgccaacg aatagccgcc agccgaatca
    19741 atagcgtcac gaccatgccc atcatactgg ctgtttccag tggtacggaa aatgtgtaat
    19801 aagccgtagc gtggacaata ccgccgataa tacaggcagt tgcgtagatt tctgtacgta
    19861 aaatcatggg gatctcgcgg gccagtacat cacgaatgat cccgccgcca acgccagtaa
    19921 tgacgaccat acaaaccgcg attaacggac cgacttccgc attaaaggct ttattcacgc
    19981 caatgccgac aaacaccgcc agaccaacgg cgtccagcac cggcaacatc cttttggtaa
    20041 gcgtcttggc tggcgcacca gcacgatggt cagcatgctg gttaccattg caacgaccag
    20101 atcggtggga tctttcaccc aaaataccgg gccgtgatcc agcgccatgt cgcgaattgt
    20161 cccgccgcct acagcggtaa ccacgcccag taccagaaca ccaaaagggt ccatacgcaa
    20221 ttttccggct aacaaaacgc cggagatggc aaataccgct gtgccgacta tatccagcca
    20281 atagacgagc attgttcaat ccccactgag cacctaaaaa ggtcaggcgc taatctacct
    20341 gtgaaagcgc attacagagc tgttgtgcag cgaggataat acgtgggctt gcacgttcaa
    20401 accagtcact cgtgagagga ataacgggaa ttttgagctg ttcaccccag tattgtttga
    20461 ttttaggaat ttggtccggt ccgcctgtaa tgacaatcgc ctgtggcgag cgtgctaaca
    20521 cctgttcgcg gctaacttgc ggccagggaa cccggctgtc tttaaagatg ttttctccgc
    20581 cacaaacttc gagtacctgg ttttgaatcg actcttttcc actggtaaat ggcggattaa
    20641 tgccgaattg cagaaaaaca cgttttttag gtttatcagc atattgcgct ttcaattgcg
    20701 cgtactgatc cagcagggat tgcgcggctt gttcggcctt atccggttgc ggactccagg
    20761 gggccaattg acgtaacgca ttggcaattt gttcaatgct tgtcgcatcg acccacatca
    20821 cttttattcc cagcgaagcc agctggtcaa cctgccgctc ggcattacct ccacgccagg
    20881 caatcaccag atcgggtttc agcgcgacaa tgcgttccag attcatcccc tgccaggtgg
    20941 aaacctgctc aatcttttgc gcttgtggag gatagtcgga atagctgctg accccaaccg
    21001 gcgtgatccc ggcggcaaag gcaagttcag tgttggcggg agaaagcgtg atgacgcgcg
    21061 gcgcggcgtt gagccacagt ggcgcaagaa aagacagggc gaccagcgcc ctgaacagtg
    21121 acttagccat gtgccagttt ctgcaccagt gactcaacca tcaggctgga ctgtttagcg
    21181 gcaacagcca ggaactcatc gaagctaaga tgagactgtt gatcggccac gtcggagatg
    21241 gcgcgtacga caacaaacgg gacgttgaaa ttgtggcaga catgggcgat tgccgtcgct
    21301 tccatctcta cagcaatggc ctgtgggaag ttgtggcgga ttttcgccag accaacagaa
    21361 ccgttgatga aagcgtcgcc gctaacaatc aggccacgta cagcgttaag attcagttcg
    21421 gcaatgcagg cctcagcggc agcgatcagt ttatcgtcag ctttaaagcc tgccggacag
    21481 cctggtaact gaccgtattc ataaccaaat gccgtgacat ccgcgtcgtg ataacgcgct
    21541 tcgtccgaga caacgatatc gcccactttc agcgttggtg ccaggccacc ggcagaaccg
    21601 gtgttaataa tcacatctgg cttgcagtgt tccaacagca gagtggcacc cagcgccgca
    21661 gcgactttac cgatgcccga tttcagaagc gcaacctcgg ttccattcag ttggccggta
    21721 tagatttcgc aaccgccgag actgatagtt tgacggtttt cgattttgtc acgcagcagc
    21781 gtaacttctt cttccattgc accaatgatg ccgattttca tagatttact cgcgataagc
    21841 ccgatttgaa agcatagttt accatgcgct tacggggaag cgtatttctc acgcgggaga
    21901 ggacatggca cagattgatt tccgaaaaaa aataaactgg catcgtcgtt accgttcacc
    21961 gcagggcgtt aaaaccgaac atgagatcct gcggatcttc gagagcgatc gcgggcgtat
    22021 catcaactct ccggcaattc gtcgtctgca acaaaagacc caggtttttc cactggagcg
    22081 caatgccgcc gtgcgcacgc gtcttaccca ctcgatggaa gtccagcagg tgggacgcta
    22141 catcgccaaa gaaattttaa gccgtctgaa agagcttaaa ttgctggaag catacggcct
    22201 ggatgaactg accggcccct ttgaaagcat tgttgagatg tcatgcctga tgcacgatat
    22261 cggcaatccg ccgtttggtc attttggcga agcagcgata aatgactggt ttcgccaacg
    22321 tttgcacccg gaagatgccg aaagccagcc tctgactgac gatcgctgca gcgtggcggc
    22381 actacgttta cgggacgggg aagaacagct taacgagctg cggcgcaaga ttcgtcagga
    22441 cttatgtcat tttgagggga atgcacaagg cattcgtctg gtgcatacat tgatgcggat
    22501 gaatctcact tgggcacagg ttggcggtat tttaaaatat acccgtccgg cgtggtggcg
    22561 tggcgaaacg cctgagacac atcactattt aatgaaaaag ccgggttatt atctttctga
    22621 agaagcctat attgcccggt tgcgtaaaga acttaatttg gcgctttaca gtcgttttcc
    22681 attaacgtgg attatggaag ctgccgacga catctcctat tgtgtggcag a
//
LOCUS       DAEEKQ010000056        22592 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.056, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000056 DAEEKQ010000000
VERSION     DAEEKQ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22592)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22592)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22592
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.056"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            235..1341
                     /gene="ompC"
                     /locus_tag="KLK57_002050"
     CDS             235..1341
                     /gene="ompC"
                     /locus_tag="KLK57_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBE0892416.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKSEDGDQTYVRLGFKGVTQVTDQLTGYGQWEYQIQGNTSEDNKENSWTRVAFAGL
                     KFQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFG
                     LVDGLNFAVQYQGKNGSVSGEGMTNNGRGALRQNGDGVGGSITYDYEGFGIGGAISSS
                     KRTDDQNSPLYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQN
                     FEAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMST
                     YVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1453..2508
                     /gene="apbE"
                     /locus_tag="KLK57_002051"
     CDS             1453..2508
                     /gene="apbE"
                     /locus_tag="KLK57_002051"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HBE0892417.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGTKTDGAMDITVGPLVNLWGFGPEQQSVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLNVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGLPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2582..3646
                     /gene="ada"
                     /locus_tag="KLK57_002052"
     CDS             2582..3646
                     /gene="ada"
                     /locus_tag="KLK57_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HBE0892418.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3646..4296
                     /gene="alkB"
                     /locus_tag="KLK57_002053"
     CDS             3646..4296
                     /gene="alkB"
                     /locus_tag="KLK57_002053"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HBE0892419.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTGDCRYNLTFRQAGKKE"
     gene            4372..6015
                     /gene="yojI"
                     /locus_tag="KLK57_002054"
     CDS             4372..6015
                     /gene="yojI"
                     /locus_tag="KLK57_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HBE0892420.1"
                     /translation="MELLVLVWRQYRWPFISVMELSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAITLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAVT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6233..7879
                     /gene="mqo"
                     /locus_tag="KLK57_002055"
     CDS             6233..7879
                     /gene="mqo"
                     /locus_tag="KLK57_002055"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HBE0892421.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDADKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNGDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8188..8676)
                     /gene="eco"
                     /locus_tag="KLK57_002056"
     CDS             complement(8188..8676)
                     /gene="eco"
                     /locus_tag="KLK57_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HBE0892422.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLTGQTLEVDCNLHRLGGKLENKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
     gene            complement(8811..8975)
                     /locus_tag="KLK57_002057"
     CDS             complement(8811..8975)
                     /locus_tag="KLK57_002057"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892423.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            9084..9578
                     /gene="napF"
                     /locus_tag="KLK57_002058"
     CDS             9084..9578
                     /gene="napF"
                     /locus_tag="KLK57_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HBE0892424.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECCFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            9568..9831
                     /gene="napD"
                     /locus_tag="KLK57_002059"
     CDS             9568..9831
                     /gene="napD"
                     /locus_tag="KLK57_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HBE0892425.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            9828..12314
                     /gene="napA"
                     /locus_tag="KLK57_002060"
     CDS             9828..12314
                     /gene="napA"
                     /locus_tag="KLK57_002060"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HBE0892426.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            12321..13016
                     /gene="napG"
                     /locus_tag="KLK57_002061"
     CDS             12321..13016
                     /gene="napG"
                     /locus_tag="KLK57_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HBE0892427.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            13003..13866
                     /gene="napH"
                     /locus_tag="KLK57_002062"
     CDS             13003..13866
                     /gene="napH"
                     /locus_tag="KLK57_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HBE0892428.1"
                     /translation="MANRKRDAGREALVKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSCSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            13863..14312
                     /gene="napB"
                     /locus_tag="KLK57_002063"
     CDS             13863..14312
                     /gene="napB"
                     /locus_tag="KLK57_002063"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HBE0892429.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            14322..14924
                     /gene="napC"
                     /locus_tag="KLK57_002064"
     CDS             14322..14924
                     /gene="napC"
                     /locus_tag="KLK57_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HBE0892430.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            14943..15560
                     /gene="ccmA"
                     /locus_tag="KLK57_002065"
     CDS             14943..15560
                     /gene="ccmA"
                     /locus_tag="KLK57_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HBE0892431.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFDDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            15557..16219
                     /gene="ccmB"
                     /locus_tag="KLK57_002066"
     CDS             15557..16219
                     /gene="ccmB"
                     /locus_tag="KLK57_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HBE0892432.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            16261..16998
                     /gene="ccmC"
                     /locus_tag="KLK57_002067"
     CDS             16261..16998
                     /gene="ccmC"
                     /locus_tag="KLK57_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HBE0892433.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            16995..17204
                     /gene="ccmD"
                     /locus_tag="KLK57_002068"
     CDS             16995..17204
                     /gene="ccmD"
                     /locus_tag="KLK57_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HBE0892434.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            17201..17680
                     /gene="ccmE"
                     /locus_tag="KLK57_002069"
     CDS             17201..17680
                     /gene="ccmE"
                     /locus_tag="KLK57_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HBE0892435.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMLGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            17677..19620
                     /gene="ccmF"
                     /locus_tag="KLK57_002070"
     CDS             17677..19620
                     /gene="ccmF"
                     /locus_tag="KLK57_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HBE0892436.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMIASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASYSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSIDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            19617..20174
                     /gene="dsbE"
                     /locus_tag="KLK57_002071"
     CDS             19617..20174
                     /gene="dsbE"
                     /locus_tag="KLK57_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HBE0892437.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            20171..21223
                     /gene="ccmH"
                     /locus_tag="KLK57_002072"
     CDS             20171..21223
                     /gene="ccmH"
                     /locus_tag="KLK57_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HBE0892438.1"
                     /translation="MRFLLGMLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVAYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIDGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(21258..21905)
                     /gene="narP"
                     /locus_tag="KLK57_002073"
     CDS             complement(21258..21905)
                     /gene="narP"
                     /locus_tag="KLK57_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HBE0892439.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            22225..>22592
                     /locus_tag="KLK57_002074"
     CDS             22225..>22592
                     /locus_tag="KLK57_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0892440.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVGLE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAVNQTTLNNGVLQVYG"
BASE COUNT         5058 a         5667 c         6427 g         5440 t
ORIGIN      
        1 tgatgttagg tgcttatttc gccattccgc aataatctta aaaagttccc ttgcatttac
       61 attttgaaac atctatagcg ataaatgaaa catcttaaaa gttttagtat catattcgtg
      121 ttggattatt ctgcattttt ggggagaatg gacttgccga ctgattaatg agggttaatc
      181 agtatgcagt ggcataaaaa agcaaataaa ggcatataac agagggttaa taacatgaaa
      241 gttaaagtac tgtccctcct ggtcccagct ctgctggtag caggcgcagc aaacgctgct
      301 gaagtttaca acaaagacgg caacaaatta gatctgtacg gtaaagtaga cggcctgcac
      361 tatttctctg acaacaagtc agaagacggc gaccagacct atgtacgtct tggtttcaaa
      421 ggcgtaactc aggttactga ccagctgacc ggttacggcc agtgggaata tcagatccag
      481 ggcaatacct ctgaagacaa caaagaaaac tcctggaccc gtgtggcatt cgcaggtctg
      541 aaattccagg atgtgggttc tttcgactac ggtcgtaact acggcgttgt ttacgacgta
      601 acttcttgga ccgacgtact gccagaattc ggtggcgaca cctacggttc tgacaacttc
      661 atgcagcagc gtggtaacgg cttcgcgacc taccgtaaca ctgacttctt cggtctggtt
      721 gacggcctga actttgctgt tcagtaccag ggtaaaaacg gtagcgtaag cggcgaaggc
      781 atgaccaaca acggtcgtgg tgctctgcgt cagaacggcg acggcgttgg cggatctatc
      841 acttatgatt acgaaggctt cggtatcggt ggtgcgatct ccagctccaa acgtactgat
      901 gatcagaaca gcccgctgta catcggtaac ggcgaccgtg ctgaaaccta caccggtggt
      961 ctgaaatacg acgctaacaa catctacctg gctgctcagt acacccagac ctataacgca
     1021 actcgcgtag gttccctggg ttgggcgaac aaagcacaga actttgaagc tgttgctcag
     1081 taccagttcg acttcggtct gcgtccgtct gtagcatacc tgcagtctaa aggtaaaaac
     1141 ctgggtacca tcgctggtcg taactacgac gacgaagata tcctgaaata tgttgatgtt
     1201 ggtgctacct actacttcaa caaaaacatg tccacctacg ttgactacaa aatcaacctg
     1261 ctggacgaca accagttcac tcgtgacgct ggcatcaaca ctgataacat cgtagctctg
     1321 ggtctggttt accagttcta atctcgattg atatcgaaca aagggcctgc gggccctttt
     1381 ttcattgttt tcagcgtaca aactcatttt tttggtgtac tcttgcgacc gttcgcatga
     1441 ggataatcac gtatggaaat aagctttacc cgcgtggcac tgctggctgc cgcgctcttc
     1501 tttgttggtt gcgatcaaaa accacaaccc gccaaaaccc acgctactga agttaccgtt
     1561 ctagaaggca aaaccatggg taccttctgg cgtgccagca tcccgggcat tgatgccaaa
     1621 cgcagtgccg aacttaaaga aaagattcag acccagctgg acgccgacga tcagctgctt
     1681 tcgacctata aaaaagattc cgcgctgatg cgctttaacg attcgcaaag tttatcgccg
     1741 tggccggtaa gtgaagcgat ggccgatatc gtcaccacct cgctgcgcat tggcacgaag
     1801 accgatggcg cgatggatat aaccgtcggg ccgctggtga atctgtgggg ctttggcccg
     1861 gaacaacagt cggttcaaat tccgagccag gaacagatcg atgcgatgaa agccaaaacc
     1921 ggcttacagc acctgacggt cattaatcag tcgcatcagc aatatctgca aaaagacctg
     1981 ccggatttaa atgtcgatct ctccaccgtc ggtgaaggtt atgcggcgga tcacctggca
     2041 cgcttgatgg agcaggaagg gatttcccgc tatctggtgt cggtgggcgg cgcgctgaac
     2101 agccgtggta tgaacggtga aggcctgccg tggcgggtag cgattcaaaa accaaccgat
     2161 aaagaaaacg cggttcaggc tgtggtggat atcaacggtc atgggattag cacctctggc
     2221 agctaccgta actattacga actggacggc aaacgtcttt cccatgttat cgatccgcaa
     2281 accgggcgtc ccatcgaaca caatctggta tccgtgacgg tgattgctcc gacggcgctg
     2341 gaagccgatg cctgggatac tggcttgatg gtactcgggc cggagaaagc caaagaggtt
     2401 gttcgccggg aagggctggc ggtttatatg atcaccaaag aaggcgatag ctttaaaacc
     2461 tggatgtcac cacagtttaa aagcttcctt atcagcgaaa aaaattaaag cgcaagattg
     2521 ttggtttttg cgtgatggtg accgggcaga ctaaaggcta tccttaacca gggagctgat
     2581 tatgaaaaac gccacatgct taactgacga tcaacgctgg caatctgtct tagcccgcga
     2641 cccgaatgcc gacggcgaat tcgttttcgc cgtgcgcacc acgggtatct tttgccgtcc
     2701 gtcttgccgc gccagacatg ccttgcggga aaatgtctcc ttctacgcaa atgccagcga
     2761 ggcactcgcc gccggatttc gcccctgcaa acgttgtcag ccagacaaag ccaatccccg
     2821 gcaacatcgg ttggataaaa tcacccacgc gtgtcgactg ctggaacagg aaacgcctgt
     2881 aacgctggaa gccttagccg accaggtggc gatgagtcca tttcatctac atcggttgtt
     2941 taaagcgact accggaatga cgcctaaagc ctggcaacag gcctggcgcg ctcgccgttt
     3001 gcgcgaatcg ctggcgaaag gggagagcgt gacgacgtct attcttaacg ccggattccc
     3061 cgacagcagc agttactacc gcaaagccga tgaaacgctg ggcatgacgg ctaaacaatt
     3121 ccgtcacggt ggcgaaaatc tggcggtgcg ttacgcgctg gctgattgtg agctgggtcg
     3181 ttgcctggtg gcagaaagcg agcgggggat ttgcgcgata ttgctgggcg atgatgacgc
     3241 gacattaatc agcgagttgc agcagatgtt tcccgctgcc gacaacgcgc ctgccgatct
     3301 gacgtttcag caacatgtgc gtgaagtgat cgccagcctc aatcaacgcg atacgccgct
     3361 gacgttaccg ctggacattc gcggcactgc ttttcagcaa caagtctggc aggcactgcg
     3421 cacgatacct tgtggtgaaa ccgtcagtta tcagcaactg gctaacgcca tcggcaaacc
     3481 gaaagcggta cgggccgttg ccagcgcctg tgccgccaac aagctggcta tcgtaatacc
     3541 ctgtcatcga gtggtccgtg gtgatggcac actttccggt taccgctggg gcgtgtcgcg
     3601 taaagcgcaa ctgctgcgcc gcgaagctga aaatgaggag aggtaatgtt ggatctgttt
     3661 gccgatgctg aaccgtggca agagccactg gcggctggtg cggtaatttt acggcgtttt
     3721 gcttttaacg ctgcggagca actgatccgc gatattaatg acgttgccag ccagtcgccg
     3781 tttcgccaga tggtcacccc cgggggatat accatgtcgg tggcgatgac caactgtggg
     3841 catctgggct ggacgaccca tcggcaaggt tatctctatt cgcccattga tccgcaaaca
     3901 aataaaccgt ggcccgccat gccacaaagc tttcatgatt tatgtcaacg tgcggctacg
     3961 gcggcgggct atccagattt ccagccagat gcttgtctta tcaaccgcta cgctcctggc
     4021 gcgaaactgt cgctgcatca ggataaagac gaaccggatc tgcgcgcgcc gattgtttct
     4081 gtttctcttg gcttacccgc gatttttcaa tttggtggcc tgaaacgtaa tgatccgctc
     4141 aaacgtttgt tgttggaaca tggcgatgtg gtggtatggg gcggtgaatc gcggctgttt
     4201 tatcacggca ttcaaccgct gaaagcgggg tttcatccac tcaccggcga ctgccgttac
     4261 aacctgacat tccgtcaggc aggtaaaaaa gaataaaaat aagaattatt attgctgtgc
     4321 gcgcgaagat gtttaaactg cgggctgtaa ttcattgtcc gggttttctg catggaactt
     4381 cttgtacttg tctggcggca gtatcgctgg ccatttatca gtgtgatgga gctaagcctc
     4441 gccagtgcgg ctttaggcat tggcttaatt gcttttatca atcagcgcct tatcgaaacg
     4501 gcggatacca gtctgctggt gttgccggag tttctgggat tattgctgct gttgatggca
     4561 atcactctcg gatcgcaact ggcgctcacc actttggggc atcacttcgt ttaccgactg
     4621 cgtagtgaat ttatcaagcg gattctcgat acccacgtcg agcgcattga acaactcggt
     4681 agcgcctcgt tgctggcggg attaaccagc gatgtgcgca atatcaccat tgcttttgtg
     4741 cgtctgccgg aactggtgca ggggatcatt ctcactatcg gttcagcggc gtatctgtgg
     4801 atgctgtcgg gcaaaatgtt gctggtaacg gctatctgga tggcggtcac catctggggc
     4861 ggttttgtgc tggtggcgcg ggtgtacaaa catatggcga ccctgcgtga aaccgaagat
     4921 aagctgtaca cggattttca aaccgtactc gaagggcgca aagagctgac tctgaaccgt
     4981 gaacgcgccg agtatgtgtt taacaacctc tacattcctg atgcgcaaga gtatcgccat
     5041 catattatcc gcgcagacac cttccatctt agtgccgtga actggtcaaa catcatgatg
     5101 ctgggcgcta tcggcctggt gttctggatg gcgaacagcc tcggttgggc tgataccaac
     5161 gttgctgcga cctattcgtt gacgctgtta ttcctgcgta cgccgctgct ttcggcggtt
     5221 ggcgcattgc cgacgctgct gacggcgcag gtggcgttta acaagctgaa caaattcgcg
     5281 ctcgcgcctt tcaaagcaga gtttccgcgc ccgcaggcgt ttcccaactg gcaaacgctg
     5341 gagctgcgta atgtgacgtt tagctatcag gataacgcgt tttccgttgg gccgattaac
     5401 ctcaccatta aacgtggcga gctgctgttt ctgattggtg gcaacggtag tggaaaatcg
     5461 acgctggcga tgttgctgac gggcttgtac cagccacaaa gcggcgaaat cttgctggat
     5521 ggcaaacctg tcagcggcga acaaccggaa gattatcgca aactgttttc ggcagtgttt
     5581 accgatgtct ggctgtttga tcaactgctg gggccggagg ggcaacccgc taacccgcaa
     5641 ctggttgaga agtggctggc gcagctgaaa atggctcata agcttgagtt aagcaacggg
     5701 cgtattgtta acctgaagtt atcaaaaggg cagaaaaaac gcgtggcgct gttgctggcg
     5761 ctggcagaag aacgcgatat tatcctgctg gatgaatggg cggcggatca ggatccacac
     5821 ttccgtcgtg agttttatca ggtgttgctg ccgctgatgc aggagatggg taaaactatt
     5881 ttcgccatca gtcatgatga tcattacttt atccacgccg accgcctgct ggaaatgcgc
     5941 aatgggcaac ttagcgagct gacgggcgaa gagcgcgatg ccgcttcgcg tgatgccgtt
     6001 gcccggacgg cataatatta cgacgctgac ctggcggact gctgccgcca ggtcaatatt
     6061 ctaaaaatcc cttcatttct aataacccta taattaatca acgaaattat aatgtttcta
     6121 aaattagaat ataatttata aacattattt aaatgttgtt actttagtgt taccgttgat
     6181 gccgcgcaaa tctcacctgc aataaagcga ctaaaagtaa ggcattaaca agatgaaaaa
     6241 agtgactgcc atgctcttct cgatggccgt ggggcttaat gccgtttcga tggcggcaaa
     6301 agcgaaagcg tccgaggagc aggaaactga tgtactgttg attggcggcg gcattatgag
     6361 cgccacgttg gggacctatt tacgcgagct ggagcctgaa tggtcgatga ccatggtgga
     6421 gcgcctggag ggtgtcgcgc aggagagttc gaacggctgg aacaacgccg gaaccgggca
     6481 ttctgcactg atggaactga actacacccc gcaaaacgcc gatggcagta tcagtattga
     6541 aaaagcagtc gccattaacg aagcatttca gatttcccgc cagttctggg cgcaccaggt
     6601 cgagcgcggc gtgctgcgta ctccgcgttc atttatcaat accgttccgc atatgagctt
     6661 tgtctggggc gaggataacg ttaatttcct gcgcgcccgt tacgccgcgt tgcaacaaag
     6721 ctcgctgttt cgcggtatgc gttactctga agatcacgcg cagatcaaag agtgggcacc
     6781 gttagtgatg gaagggcgcg atccgcaaca gaaagtggca gccacgcgta cggaaattgg
     6841 taccgatgtg aactacggcg agatcacccg ccagttaatt gcttccttgc agaagaaatc
     6901 taacttctcg ctgcaactca gcagcgaagt ccgcgcccta aagcgtaatg acgataacac
     6961 ctggaccgtt accgttgccg atctgaaaaa tggcactgca cagaacattc gtgccaaatt
     7021 tgtctttatc ggcgcgggcg gtgcggcgct gaagctgtta caggaatcgg ggattccgga
     7081 agcgaaagac tacgccggtt tcccggtggg cggacagttc cttgtttcgg aaaacccgga
     7141 cgtggttaat caccatctgg cgaaggttta cggtaaagca tccgttggcg caccaccgat
     7201 gtcggttccg catatcgata cccgcgttct ggacggtaaa cgcgtagtgc tgtttgggcc
     7261 atttgccacc ttctcaacca aattcctcaa aaacggttca ttgtgggatc tgatgagttc
     7321 caccaccacc tctaacgtga tgccgatgat gcacgtcggg ctggataatt tcgatctggt
     7381 gaaatacttg gtgagtcagg tgatgttgag tgaagaggat cgttttgaag cgttgaaaga
     7441 gtactatccg caggcgaaaa aagaggactg gcgtttgtgg caagcggggc agcgtgtgca
     7501 gattatcaag cgtgatgccg ataaaggtgg tgtactgcgt ctgggtactg aagtggtcag
     7561 tgaccagcaa gggaccattg ccgcgctgct gggggcatcg ccaggggcgt caaccgctgc
     7621 gccgattatg ttggatctgc tggaaaaagt atttggcgat cgcgtttcca gcccgcaatg
     7681 gcaggctacg ttgaaagcga tcgttccatc ttatggacgc aagctgaacg gtgatgtagc
     7741 ggcaacagaa cgcgagttgc agtacaccag cgaagtgctg ggcctgaaat acgacaagcc
     7801 gcaggcagca gatagtacgc cgaaaccgca gttgaaaccg caacccgttc aaaaagaagt
     7861 ggcggatatt gcgttgtaat gatgtgccac atccggcatg gtatgccgga tgtggcgtaa
     7921 ccgccttatc cgacctacgg ggtggagcac ttcacagcgg tgtaggcctg ataagacgcg
     7981 tcagcgtcgc atcaggcaac cggctcgggc gttagatgtc ggatgcggcg taaccgcctt
     8041 atccaaccta cggggtggag cacttcacag cggtgtaggc ctgataagac gcgccagcgt
     8101 cgcatcaggc aaccggcttg ggcgttagat gtcggatgcg gcgtgaccgc cttatccgac
     8161 ctacggggtg acgcacttca cagcagttta gcgaactacc gcgttgtcaa ttttctcttc
     8221 cgccttccag acgcggtact tcacatctac attgtctggc gtatacacca cgatcggcag
     8281 cttgctgttg taacgcagca ttccagcatc gcccagatac gcggtgacaa atttcttctc
     8341 tttcttgcca tccgggcagg ccatcatcgt ggaaaccggc gagctaactt tatcaaagac
     8401 ataataatca tagccccagc cttccagcgt tttgttttcc agcttcccgc cgagacgatg
     8461 caaattgcaa tcgacttcca gcgtctgacc ggttaacagt tctactttca gggtagattc
     8521 atcttcttgc ggggttaact gaatcacctg acgcttcatc cctttttcag cttgtggata
     8581 aggcgcgatt ttttccagtg gctggacgct ttctgccgcc caggcggaag tggtggcgaa
     8641 agcggcaaac aatactgcag gtagaatggt cttcattttt ttgctttcct tcttcattta
     8701 taggttttca ccgatgatgt cgctgaaggt taacatattc atcagattta ttaatttgct
     8761 ttatgaataa cttaaagaac attcagacaa ttttacctgc tcttttagtg ttaaattctg
     8821 atgagggagc aaaccagcag aaatatctct ggtgaattaa tcgctatata aatatgttta
     8881 taaccatttg aaatgtgagc aaaagcccgt ttttgccacg cttcccgcca ctcttttgat
     8941 cctgctacag gttttacccc gatcggggta tgcatctttg acacatcctt taatatctta
     9001 gcggctataa aaatggctta ttaattatgc ggtttatttg gtcgctctca attttcagag
     9061 cgcgttaatg atggaaggtc aatgtgaaga ttgatgcatc ccgtcggggc atactcactg
     9121 gtcgctggcg caaagccagt aacggtatcc gtccgccctg gtccggtgat gaatctcatt
     9181 ttctgaccca ttgcacccgt tgcgacgcct gtattaatgc ttgtgaaaac aacattctgc
     9241 aacgcggcgc gggcggctat ccgagcgtta atttcaaaaa taacgagtgc tgcttctgtt
     9301 acgcctgcgc gcaggcttgc cccgaatcgc tattttctcc gcgccacacc agggcctggg
     9361 atttgcagtt caccattggg gacgcgtgcc tggcgtatca gtcagttgaa tgccgccgct
     9421 gtcaggatag ctgtgagcca atggcaatta tctttcgccc gacgctctcc gggatctacc
     9481 agccgcaact taatagccaa ctttgcaacg gatgtggagc atgtgcagcc agctgtccgg
     9541 tatcagccat aactgcggag tatctccatg cacactaact ggcaagtttg cagcctggtc
     9601 gtgcaggcca aaagcgaacg aatttcagac atcagcaccc aactgaacgc ctttcccggc
     9661 tgtgaagttg ctgtcagcga cgcgccgagc ggtcagttga ttgtggtggt ggaagcagaa
     9721 gacagcgaaa cgctgatcca aaccattgag tcagtacgca acgtagaggg cgtgctggcg
     9781 gtgtcgctgg tttatcacca gcaggaagag caaggtgagg aaacaccatg aaactcagtc
     9841 gtcgtagctt tatgaaagct aacgccgttg cggccgctgc ggcggctgcc ggtctcagcg
     9901 tgccgggcgt tgcccgcgcc gttgttggtc agcaggaagc cattaaatgg gataaagcgc
     9961 cgtgccgttt ctgcggtact ggttgcggcg ttctggtcgg aacgcagcag gggcgtgtgg
    10021 tggcctgtca gggcgacccg gacgcaccgg ttaaccgtgg cctgaactgc attaagggct
    10081 atttcctgcc caagatcatg tacggtaaag accgtttgac gcagccgctg ctgcgtatga
    10141 aaaacggtaa atatgacaaa gaaggcgaat ttaccccaat cacctgggat caggccttcg
    10201 atgtgatgga agagaaattc aaaaccgccc tgaaagaaaa agggccggaa tcgatcggta
    10261 tgttcggttc tggtcagtgg actatctggg aaggttatgc cgcgtccaag ctgtttaaag
    10321 cgggcttccg ttcgaacaac atcgacccga acgcgcgtca ctgtatggcg tcggcagtag
    10381 ttggctttat gcgtaccttt ggtatggatg agccgatggg ctgctatgac gacatcgagc
    10441 aggctgacgc gtttgtgctg tggggcgcaa acatggcgga gatgcacccg atcctctggt
    10501 cgcgcatcac taaccgtcgt ctctctaatc agaacgtcac cgtggcggtg ctttctacct
    10561 accagcatcg tagcttcgag ctggcggata acggcatcat ctttacgcca cagtctgacc
    10621 tggtgatcct gaactacatc gccaactata tcattcaaaa caatgcgata aatcaggact
    10681 tcttcagcaa acacgttaac ctgcgcaaag gggcgacgga catcggctac ggtttacgtc
    10741 cgacccatcc gctggaaaaa gcagcgaaga atccaggttc tgacgcctcc gaaccgatga
    10801 gctttgaaga ttacaaagcc ttcgttgccg agtatacgct ggaaaaaacc gctgaaatga
    10861 ccggcgtgcc gaaagaccag ttagaacaac tggcgcagct gtatgccgat ccgaacaaga
    10921 aagtcatctc ctactggacg atgggcttca accagcatac tcgtggcgtg tgggctaaca
    10981 acctggtcta caacctgcac ctgctgaccg gcaaaatttc ccagccgggt tgcggtccgt
    11041 tctccctgac cgggcaacct tctgcgtgtg gtactgcgcg tgaagtgggc acctttgctc
    11101 accgtctgcc tgcggacatg gtggtgacca acgagaaaca ccgtgatatc tgcgagaaga
    11161 agtggaatat cccgagcggc accattccgg cgaaaatcgg tctgcacgcg gtggcacaag
    11221 accgtgcgct gaaagacggc aaactgaatg tttactggac catgtgtacc aacaacatgc
    11281 aggccgggcc gaacatcaat gaagagcgta tgccgggctg gcgcgatccg cgcaacttca
    11341 tcatcgtctc cgatccgtat ccgacagtca gtgcgctggc cgccgacttg atcctgccga
    11401 ccgcaatgtg ggtagagaaa gagggtgctt acggtaacgc cgaacgccgt actcagttct
    11461 ggcgtcagca ggtacaggca ccgggcgaag cgaaatcgga tctctggcag ttagtccagt
    11521 tctcccgccg cttcaaaact gaagaagtgt ggccggaaga gctgctagcg aagaaaccgg
    11581 aactgcgtgg caaaacgctg tacgaagtgc tttatgccac accggaagtg agcaaattcc
    11641 cggtatccga actggcggaa gatcagctga acgatgaatc ccgcgagctg ggcttctatc
    11701 tgcaaaaagg gctgttcgaa gagtacgcat ggtttggtcg cggtcacggt cacgatctcg
    11761 cgccgttcga tgactaccac aaagcgcgcg gtctgcgctg gccggtggtg aacggtaaag
    11821 aaacgcagtg gcgttacagc gaaggtaacg acccgtacgt gaaagcgggc gaaggctata
    11881 agttctacgg taaaccggat ggtaaagcgg tgatcttcgc gctgccgttc gaaccggcgg
    11941 cggaagcacc ggatgaagag tacgacctgt ggctctctac cggacgagtt ctggagcact
    12001 ggcacactgg cagtatgact cgccgtgtgc cggaactgca ccgcgccttc ccggaagcgg
    12061 tcttgtttat tcacccgctg gatgcgaaag cgcgcgatct gcgccgtggc gacaaagtga
    12121 aagtggtttc tcgccgtggc gaagtgatct cgattgttga aacgcgcggt cgtaaccgtc
    12181 cgccacaggg cctggtgtac atgccgttct tcgacgccgc acagctggtt aacaaactaa
    12241 cgctggacgc gaccgatccg ctctcgaaag agacggactt caagaagtgc gcagtcaaac
    12301 tggagaaggt gtaagccatt atgtcccggt cagcgaaacc tcaaaatggt cgccgccgct
    12361 ttctgcgcga tgttgttcgc acagcaggcg ggctggctgc cgtgggtgtg gcgctggggt
    12421 tacaacagca aaccgcacgc gcatctggcg tgcggttgcg cccgcccgga gccataaacg
    12481 agaacgcctt tgccagtgcc tgtgtgcgtt gcggtcagtg tgttcaggct tgcccttacg
    12541 acaccttaaa actggcgacg ctggcttctg gcctgtcggc gggcacgcca tattttgtcg
    12601 cacgggatat tccttgcgaa atgtgtgagg acattccgtg cgccaaagtg tgcccgagtg
    12661 gtgcgctgga tcgtgagatc gaatcgatcg acgacgcgcg gatggggctg gcggtgctgg
    12721 tggaccagga aaactgtctc aactttcagg ggctgcgctg cgatgtttgt tatcgcgaat
    12781 gcccgaaaat tgatgaggcc atcaccctgg agctggagcg caacacgcgt accggtaagc
    12841 acgcccgctt tctgccgacg gttcacagcg acgcctgtac cggctgcggt aagtgcgaaa
    12901 aagtgtgcgt gctggaacaa ccggcaatca aggtgttacc gctgtcactg gcgaaagggg
    12961 agttaggtca ccattaccgc ttcggctggc tggaggggaa caatggcaaa tcgtaaacgt
    13021 gacgccgggc gcgaggcgct ggtgaaaaaa ggctggtggc gcagtcaccg ttggctggtg
    13081 ttacgtcgcc tttgtcagtt cttcgtgctg gggatgtttt tgagcggtcc gtggtttggt
    13141 gtgtggatct tacacggcaa ctacagctgt agcctgttat tcgacaccgt tccgctgacc
    13201 gatccgctga tgacgctgca aagtcttgcc agtggtcatc tgcctgccac ggtggcgttg
    13261 accggtgcgg tgataatcac tgtgctctac gccctggcag gaaagcgatt attttgcagc
    13321 tgggtctgcc cgctgaaccc gattaccgac ttagcgaact ggttacgcag gcgctttgac
    13381 ctcaatcagt ctgcgacgat ccctcgccac attcgctacg tgctgttggt ggtcattctg
    13441 ctgggatcag ccttaaccgg cacgctcatc tgggaatgga tcaaccccgt ttccctgatg
    13501 gggcgtagcc tggtaatggg cttcggcagc ggcgcgctgc ttattctcgc gctgttttta
    13561 tttgatctac tggtcgttga acacggctgg tgcgggcaca tttgtccggt tggcgcgctg
    13621 tatggcgtgc tgggtagcaa aggtgtgatt accgttgcgg ccagcgatcg gcagaaatgt
    13681 aaccgctgta tggattgttt tcatgtttgc ccggaaccgc atgtgctacg tgccccggtg
    13741 ctggatgaac aaagcccggt gcaggtcacc agccgcgatt gcatgacgtg cggtcgctgc
    13801 gtggatgtct gttctgagga tgtatttaca ataactacac gatggagttc gggagcgaaa
    13861 tcatgaaaag ccatgacctg aagaaagcgc tgtgtcaatg gacggcgatg ctggccctgg
    13921 tggtaagcgg cgcggtttgg gcggctaatg gcgtcgattt tagccagtcg ccggaagtat
    13981 ccgggacgca ggaaggggcc attcggatgc caaaagagca ggatcggatg ccgctgaact
    14041 atgtgaatca gccgccaatg atcccgcata gcgttgaagg ttatcaggta acgaccaata
    14101 ccaaccgctg cttgcagtgc cacggtgtcg aaagctatcg caccactggc gcgccgcgta
    14161 tcagtcctac tcactttatg gacagcgacg gcaaagtggg cgcggaagtg gctccgcgtc
    14221 gttatttctg tctgcaatgt cacgtaccgc aggccgatac cgcgccaatc gtggggaata
    14281 cctttacccc atcaaaaggt tacgggaaat aagaggttat tatgggaaat tctgaccgta
    14341 agcctggtct gattaagcgc ctgtggaaat ggtggcgtac ccccagccgt ctggcgctgg
    14401 gaacgctgct gttgatcggt tttgtcggcg gcatcgtctt ctggggcggc tttaacaccg
    14461 gaatggaaaa agccaatacc gaagagttct gcattagctg ccacgaaatg cgtaacacgg
    14521 tgtatcagga atacatggat tccgtgcact acaacaaccg tagcggcgtc cgtgcgacct
    14581 gtccggattg tcacgttccg cacgagtttg tgccgaagat gatacgcaag ctcaaagcaa
    14641 gtaaagagct gtatggtaaa atttttggcg ttattgacac gccgcagaaa tttgaagctc
    14701 atcgtctgac aatggcacag aatgagtggc ggcgcatgaa ggacaataac tcgcaggagt
    14761 gccgtaactg tcacaacttc gagtatatgg atacaaccgc ccagaaatcg gttgccgcga
    14821 agatgcatga ccaggcggtg aaagatgggc aaacctgtat tgattgccat aaagggatag
    14881 cgcacaagct gcccgatatg cgtgaagtcg agccaggttt ttaacagggt tattgcgtgg
    14941 gtatgcttga agccagagag ttactttgtg agcgggatga acgaacctta tttagtggct
    15001 tgtcatttac gctgaacgca ggagagtggg tacaaatcac cggtagcaac ggcgcgggga
    15061 agacaacgct tctccgtttg ctgacggggt tgtctcgccc tgacgcaggc gaggttctct
    15121 ggcaagggca gcccttgcat caggtacgcg acagctacca tcaaaacctg ttatggatag
    15181 gccatcagcc ggggatcaaa acccggctga cggcgttaga aaatctgcac ttttatcatc
    15241 gcgatggcga taccgcacaa tgtctggaag ccctggcgca ggccgggctt gccggattcg
    15301 acgatattcc ggtaaatcaa ctttctgccg ggcaacaacg ccgcgtcgct ttagcgcgtc
    15361 tgtggctgac ccgtgccacg ttatggatcc tcgacgagcc ttttaccgcg attgacgtta
    15421 acggcgtcga tcgtctgacc cagcgtatgg cgcagcatac ggagcagggg gggattgtga
    15481 ttctgactac ccaccagccg ctcaacgttg ctgaaagtaa aattcgccgc atttcactga
    15541 cgcaaacgag ggccgcatga tgttctggcg cattttccgt cttgagctgc gtgtagcgtt
    15601 tcgccatagc gccgaaatcg ccaacccgct gtggttcttc ctgattgtaa ttaccctttt
    15661 tccgctcagt atcggtccgg agccgcaact gctggcgcgt attgcaccgg gcattatctg
    15721 ggttgctgcg ctgctttcat ccttgctggc gctggaacga ctgttccgtg acgatttgca
    15781 ggacggcagt cttgaacaat tgatgttgtt gccgttaccc ttgcccgccg ttgtgctggc
    15841 gaaggtgatg gcgcactgga tggtaaccgg tctgccgtta ctcatccttt cgccactggt
    15901 agcaatgcta ctgggaatgg atgtttatgg ctggcaagtg atggcgctga cgctgctgct
    15961 gggaacgcct acgcttggct ttctcggtgc accgggtgtg gcgttgacag tgggacttaa
    16021 gcgcggtggt gtgctgctca gcatactggt gttaccgctg actatcccat tactcatctt
    16081 tgccaccgcc gcgatggacg cggcttccat gcatttgccc gttgacgggt atctggcaat
    16141 tttaggcgcg ttgctggcag gcaccgcgac attaagtcct tttgcgacgg cggcagcgtt
    16201 acgaatcagc attcaataac gctggattac tgatttttgt tcgagtctgg tatcgaaact
    16261 atgtggaaaa cactgcatca actggcgatc ccaccacggc tgtatcaaat ctgtggctgg
    16321 tttataccgt ggctggcaat tgccagtgtg gtcgtgctta ccgtcggctg gatctgggga
    16381 ttcggctttg ctccggctga ttatcagcag ggaaatagct accgcattat ctacctgcat
    16441 gtgcctgcgg cgatctggtc gatgggcatt tatgcatcaa tggcagtggc agcgtttatt
    16501 ggccttgtct ggcagatgaa aatggccaac ctggcggtgg cggcgatggc ccccattggt
    16561 gccgtgttta cctttattgc cctggttacc ggctctgcat ggggaaaacc gatgtggggc
    16621 acctggtggg tatgggatgc acgtctgact tctgaactgg tgctgctgtt tttgtatgtg
    16681 ggtgtgattg ccctgtggca cgccttcgac gaccgccgtc tggcgggccg tgcggcaggt
    16741 atcctggtgc tgattggcgt ggtgaatctg ccgattattc attactccgt ggagtggtgg
    16801 aacaccctgc atcagggatc aacgcggatg cagcaaagta tcgatccggc gatgcgttcg
    16861 ccgctgcgct ggtcgatttt tggcttcctg ctcctgtctg ccacgctgac gctgatgcgg
    16921 atgcgtaatt tgattttgct gatggaaaaa cgccgtccgt gggtgagtga actgatactg
    16981 aaaagaggcc gtaaatgacc cctgcatttg cttcctggaa tgaatttttc gcaatgggcg
    17041 gttacgcctt ttttgtctgg ctggcggtgg tgatgaccgt tattccgctg gtggttttgg
    17101 tcgtgcactc ggtgatgcaa catcgcgcaa ttctgcgtgg tgtggcgcaa cagcgggcgc
    17161 gtgaggcgcg tttacgtgct gcgcaacagc aggaggctgc atgaatattc gccgtaaaaa
    17221 ccgcttgtgg attgcctgtg ccgtgttggc agggctggcg ctgactatcg gtctggtgct
    17281 atatgcgctg cgctcgaata tcgatctctt ttatacgccg ggggaaattc tctacggcaa
    17341 gcgtgaaact cagcaaatgc cggaagtcgg tcagcgtttg cgcgttggcg ggatggtgat
    17401 gctgggtagt gtgcagcgcg atcccaattc gctgaaagtg accttcacta tttacgatgc
    17461 tgaaggctca gtggatgtct cttacgaagg cattttgccg gatctgttcc gtgaagggca
    17521 gggcgttgtg gtgcagggcg agctggaaaa aggcaatcat atcctcgcga aagaagtgct
    17581 ggcgaaacac gacgaaaact acacgccgcc agaagttgag aaagcgatgg aagctaacca
    17641 ccgtcgcccg gcgagtgttt ataaggaccc agcatcatga tgccggaaat tggaaacggg
    17701 ctgctgtgtc tggcgttagg gattgcgctg ctgctgtccg tgtatccgct atggggcgtg
    17761 gcgcgcggag atgcgcgcat gatcgcgtct tcccgcttgt ttgcctggct gctgtttatg
    17821 tctgtggctg gcgcatttct ggtgctggtc aatgctttcg tggtcaacga ctttaccgtc
    17881 acctatgttg ccagctactc caatacccag cttccggtgt ggtatcgcgt ggcggctacc
    17941 tggggcgcgc atgaaggctc gctactgctg tgggtgctgc tgatgagcgg ctggaccttt
    18001 gcggtggcga tttttagtca gcgtattccg ctggatattg tggcccgcgt actggcgata
    18061 atggggatgg tcagcgtcgg ctttttgctg ttcattctct ttacctctaa cccattctca
    18121 cgcacgctgc cgaacttccc gattgaaggg cgcgatctta acccgctgtt acaggacccg
    18181 gggctgatct tccatccgcc tctgctttat atggggtacg tgggtttctc ggtggcgttt
    18241 gcttttgcca ttgcttcttt gctgagcggg cgtctggaca gcacttatgc gcgttttact
    18301 cgcccgtgga cgctggcggc gtggattttc ctgacgctcg gcattgtgct cggttccgca
    18361 tgggcctatt acgaactcgg ctgggggggc tggtggttct gggatccggt agaaaacgcc
    18421 tcgtttatgc cgtggctggt ggggactgcg ctgatgcact cactggcggt cactgaacaa
    18481 cgcgccagct tcaaagcgtg gacattactg ctggcaatca gtgccttctc gttgtgtctg
    18541 ttggggactt tcctcgtgcg ttccggcgtg ctggtatcgg tacacgcgtt tgcgtctgat
    18601 ccggcgcgcg gtatgtttat cctcgccttt atggtgctgg tgattggagg ttcgctgctg
    18661 ctgtttgccg cgcgtggaca caaagttcgc tcacgcgtaa acaatgcgct gtggtcgcgg
    18721 gaatctctgc tgttagcgaa caacgttttg ctggtcgccg cgatgctggt ggtgttgctg
    18781 gggacgctgc tgccgctggt gcacaagcaa ctgggactgg gcagtatttc gattggcgaa
    18841 ccgttcttca acaccatgtt tacctggctg atggtgccgt ttgcgctact gctgggtgtc
    18901 ggtcctctgg tgcgctgggg gcgcgatcgc ccacgtaaaa tccgcaattt attgattatc
    18961 gccttcatct caacgctggt gctgtctctg ctgttgccgt ggctgtttga gagcaaagtt
    19021 gtggcgatga cggtgctcgg cctggcaatg gcctgctgga ttgcggtgct ggcaattgcg
    19081 gaagctgcgc tgcgtatttc acgcggcacg aaaaccacct tcagttattg ggggatggtg
    19141 gctgctcacc tggggctggc agtgacaatt gttggcattg cttttagcca gaactatagc
    19201 gttgagcgtg atgtgcgcat gaagtccggc gatagcatcg atattcatga atatcgcttc
    19261 accttccgtg atgtcaaaga ggtgactggc ccgaactggc gtggcggtgt ggcgactatc
    19321 ggcgtaacgc gagagggtaa accggaaaca gtgctgtatg cggaaaaacg ttattacaac
    19381 actgccgggt cgatgatgac cgaagcggcg attgacggtg gcatcacgcg tgacctgtac
    19441 gccgcgctcg gtgaagagct ggaaaacggc gcgtgggctg tgcgtcttta ctacaaacca
    19501 tttgttcgct ggatttgggc gggcgggctg atgatggcgt tgggcggact gctgtgtctg
    19561 tttgatcctc gctatcgtaa gcgcgtgagt ccgcaaaaaa ctgcgccgga ggctgtatga
    19621 agcgcaaagt attgttaatt ccgttgatta tcttcctggc gattgccgcg gcgctgctgt
    19681 ggcagctggc gcgtaatgcc gaaggggatg atccgaccaa tctggaatcg gcgctcattg
    19741 gcaagccagt gccgaagttt cgcctcgaat cactggacaa tccggggcag ttttatcagg
    19801 cggatgtgct gactcagggc aaaccagtgc tgcttaacgt ctgggcgacc tggtgtccga
    19861 cctgccgtgc ggaacatcaa tatctgaatc agctttctgc gcagggcatc cgcgtagtcg
    19921 gcatgaacta taaagacgat cgccagaagg caatcagctg gctgaaagag ctgggcaatc
    19981 cttacgcgct aagcctgttt gatggcgatg gcatgttagg gctggatctc ggtgtctatg
    20041 gcgcgccaga aacgttcctt attgacggca acggcatcat tcgctatcgc catgcgggcg
    20101 atctcaatcc tcgcgtctgg gaagaagaga tcaagccgct gtgggagaaa tacagtaagg
    20161 aggccgcaca atgaggtttt tattgggcat gctgatgctg atgatctccg gctcagcgct
    20221 ggcgaccata gacgtgttgc agtttaaaga tgaagcacag gaacaacagt tccgtcagct
    20281 cactgaagaa ctgcgctgcc cgaaatgcca gaacaacagc attgccgatt ccaactcgat
    20341 gattgccacc gacctgcgcc agaaagtgta tgaactgatg caggaaggta aaagtaaaaa
    20401 agagattgtc gattatatgg tggcgcgtta cggcaacttc gtcacttacg atccgccgtt
    20461 aacgccgctg accgtgctgc tgtgggtgct gccagtagta gctattggca ttggcggttg
    20521 ggtcatatac gcccgctcgc ggcgtcgggt acgcgtggtg ccggaagcgt ttcctgaaca
    20581 aagcgtgccg gaaggtaagc gtgccggata tgtcgcttat ttgccgggca ttgtggtggc
    20641 gttaattgtg gctggcgtca gctactacca gaccggaaat tatcaacagg tgaaaatctg
    20701 gcagcaggcc acggcacagg ctccggcgtt gctggacagg gcgctggatc cgaaagctga
    20761 tccgctcaac gaagaagaga tgtcgcgcct tgcgctgggg atgcgtactc aactgcaaaa
    20821 aaatccggga gatatagacg gctggattat gttgggccgc gttggcatgg cgctgggtaa
    20881 cgccagtatc gccaccgatg catacgctac tgcgtatcgc ctcgatccga aaaacagtga
    20941 tgccgcactg ggttatgctg aagcgttgac acgttcatct gatcccaacg acaaccgcct
    21001 cggcggcgaa ctgctacgtc agctggtgag aacggaccat agcaatatcc gtgtgctaag
    21061 catgtatgcg tttaatgcct ttgagcagca gcgatttggc gaagccgttg ccgcgtggga
    21121 gatgatgttg aaactcttac ctgccaacga tactcgccgt gcggtgattg aacgtagtat
    21181 cgcgcaggcg atgcaacatt tgtcgccgca ggagagtaaa taaaaagagg cccgatggtg
    21241 atgccatcgg gctattttta ttgtgccccg cgttgttgca ggaacagaat ggtggccgcc
    21301 acgcgtgagc ggacattgag tttacgcagc agatttcgaa tatgaacttt tactgtctgc
    21361 tcggaaatat tcaacacaga ggcaatctgt ttatttgaca gcccctgtgc cagctcgtgc
    21421 agaacatcca gctcgcgctc cgtcagcacg ctgaagggat cttcttccgc gccaaacatt
    21481 tcacgttcac gtaagtactg attgacgcgt tcgctaaaga ctttgctgcc tttcgctccg
    21541 gcacgaatcg cttccagcaa cacttccggg tcgctgtctt tcagcagata accgtctgcg
    21601 cctgcgtcta tcagcgcaaa gacatcgctg gaggcatcgg atacggtcag gataataatt
    21661 tgcgcggtaa cgccatccct gcgcaaggca ttgagggtat ccaggccact catacctttc
    21721 atattgagat ccagcaagat cacgtcgata tccagtctat tcgccagatc gatcgcgctc
    21781 gcgccgtcgc ccgcttcggc gaccacttca aagccaggat caagctccag taactgacga
    21841 acaccgcgtc gcataagtgg atgatcatcc acaatcatca cctgaaaagg cgttgcttca
    21901 ggcatagtag tctcctgagg ttttattaga atcattatgt ttgagcgtag cgttggtaac
    21961 gctaccaggc gcgtgaatgt taccccattt tatgtatgga catgtagtga aaactacaac
    22021 cttgccgttc gctttggcat cattttcatt ataaagacgg aattttgcat cagcttctca
    22081 tcaagaaaaa caactaaaaa atcaatcaca atacttgtgc atttctttaa acagttttta
    22141 aaaactgact ccgggtatgg agctatgggt attttctgta cccaatgctt ttaacagcaa
    22201 ttaatttcat aggatgaaag ctcaatgcat caatctggtt ctgtttctct ttgtcgttcc
    22261 gcaatatctg ttctggtggc tacagcgtta tattcaccca tagcattggc atcaacagtt
    22321 gagtatggtg agacagttga tggtgttggc ctggaaaaag atatccagct ggtttatggg
    22381 accgccaata atacgaaaat caatcctggc ggagaacagc atataaaaga atttggtgta
    22441 agtagtaata ctgaaattaa cggcgggtat cagtacattg aaatgaatgg cactgcagaa
    22501 tactcagtat taaatgatgg ttatcaaatt gttcaaatgg gtggcgcggt aaaccagact
    22561 acgctcaata atggagtgct acaggtttat gg
//
LOCUS       DAEEKQ010000057        21643 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.057, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000057 DAEEKQ010000000
VERSION     DAEEKQ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.057"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="KLK57_002075"
     CDS             complement(<1..1127)
                     /gene="fsr"
                     /locus_tag="KLK57_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HBE0892441.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIAD"
     gene            1345..2997
                     /gene="ushA"
                     /locus_tag="KLK57_002076"
     CDS             1345..2997
                     /gene="ushA"
                     /locus_tag="KLK57_002076"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HBE0892442.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTREDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMIGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="KLK57_002077"
     CDS             complement(3034..3513)
                     /gene="ybaK"
                     /locus_tag="KLK57_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HBE0892443.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(3717..4511)
                     /locus_tag="KLK57_002078"
     CDS             complement(3717..4511)
                     /locus_tag="KLK57_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HBE0892444.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPTCEALEERINEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQITMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            4649..4990
                     /locus_tag="KLK57_002079"
     CDS             4649..4990
                     /locus_tag="KLK57_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBE0892445.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(5205..7709)
                     /gene="copA"
                     /locus_tag="KLK57_002080"
     CDS             complement(5205..7709)
                     /gene="copA"
                     /locus_tag="KLK57_002080"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HBE0892446.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARVVTADGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGESVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTIVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKTGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            7971..8903
                     /gene="glsA"
                     /locus_tag="KLK57_002081"
     CDS             7971..8903
                     /gene="glsA"
                     /locus_tag="KLK57_002081"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HBE0892447.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            8906..10198
                     /locus_tag="KLK57_002082"
     CDS             8906..10198
                     /locus_tag="KLK57_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0892448.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            10323..10730
                     /gene="cueR"
                     /locus_tag="KLK57_002083"
     CDS             10323..10730
                     /gene="cueR"
                     /locus_tag="KLK57_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HBE0892449.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(10731..11189)
                     /locus_tag="KLK57_002084"
     CDS             complement(10731..11189)
                     /locus_tag="KLK57_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HBE0892450.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(11186..12103)
                     /locus_tag="KLK57_002085"
     CDS             complement(11186..12103)
                     /locus_tag="KLK57_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HBE0892451.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGTSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            12249..12926
                     /gene="fetA"
                     /locus_tag="KLK57_002086"
     CDS             12249..12926
                     /gene="fetA"
                     /locus_tag="KLK57_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HBE0892452.1"
                     /translation="MQENSPLLQLQNVGYLAGDAKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNRQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            12913..13692
                     /gene="fetB"
                     /locus_tag="KLK57_002087"
     CDS             12913..13692
                     /gene="fetB"
                     /locus_tag="KLK57_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HBE0892453.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="KLK57_002088"
     CDS             complement(13755..14609)
                     /gene="cnoX"
                     /locus_tag="KLK57_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HBE0892454.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNFTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGMVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="KLK57_002089"
     CDS             complement(14670..15479)
                     /gene="ybbO"
                     /locus_tag="KLK57_002089"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HBE0892455.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="KLK57_002090"
     CDS             complement(15469..16092)
                     /gene="tesA"
                     /locus_tag="KLK57_002090"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HBE0892456.1"
                     /translation="MNFNNVFRWHLPFLFLALLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            16063..16749
                     /locus_tag="KLK57_002091"
     CDS             16063..16749
                     /locus_tag="KLK57_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0892457.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            16746..19160
                     /locus_tag="KLK57_002092"
     CDS             16746..19160
                     /locus_tag="KLK57_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0892458.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYDKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMQLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
     gene            complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="KLK57_002093"
     CDS             complement(19394..20488)
                     /gene="mnmH"
                     /locus_tag="KLK57_002093"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HBE0892459.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQAAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTDSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKTQ"
     gene            complement(20557..21483)
                     /gene="allS"
                     /locus_tag="KLK57_002094"
                     /pseudo
     CDS             complement(20557..21483)
                     /gene="allS"
                     /locus_tag="KLK57_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
BASE COUNT         5015 a         5684 c         5799 g         5145 t
ORIGIN      
        1 tgatcggcga taagccccag aactgccgct cccagacctc ccatgccaaa agcaaaaccg
       61 aaaaagagtc cagaaaccat accgatacgt cccggaagta gctcctgagc gtagaccaga
      121 atggcagaga atgccgaagc gaggataaat ccaataatca ccgttaaaac ccccgtccag
      181 tgcaggctgg cgtagggtaa aatcagcgta aacggcgcaa cgccgaggat agagccccaa
      241 atcacatatt tccgtccaat tttatcccct acaggcccgc cgatcaccgt acctgccgca
      301 acggcaaaca ggaaggcaaa cagatgaagc tgggcattct ggatagataa tccgaatttt
      361 tgcatcagat aaaaggtgta atagctgctg atgctcgcca tatagaaata tttcgagaaa
      421 atgaggatta acagaatgct gactgccagt accactttgt tgcgcggcag tggattgatt
      481 atcgtcgctt tgggttttcc tttattcatt cggtgctgtg ccgagtacca acggctaatt
      541 tgcgccaaca ccacgatcgc cagcagtgcc gcaagcacaa accaggcaac gttgcctttg
      601 ccataaggcg cgataatcac cgccgccagc aagggtccca gggaactgcc aaagttgccg
      661 ccgacctgaa agatagattg cgccaggcca tgccgcccgc cggaagccat acgggctacg
      721 cgagaagatt ccggatgaaa gaccgatgaa ccggtaccga ccagcgccgc cgccagcaga
      781 actgcgccaa aactgcccgc cagcgcaagc agcaccagac cacttaaggt aaagcacatg
      841 ccaattggca acgaccacgg catcggatat ttatcggtcc agtagccgac cactggttgc
      901 agtagcgaag aggcgagctg gaaggtgagg gttatcatgc caatctgcat aaatgtcaga
      961 gaaaattctg actgaagcag cggataaatc gccagaatca gcgattggat catgtcgttc
     1021 agcagatgtg agaggctgat agcacctaaa ataccaaacg atgttcgggc cttggtcgtt
     1081 gacgcagccg cgcccgccac aggctggggt tgttcactca ttgccatagg aaagtcactt
     1141 tttcagggtt gcgatgtaaa gaatgatctt atttgtgatt attacccgac taacatacct
     1201 gtatgcgtcg tctgaaggaa gtctcaacgc cgaatacaga atttctaatc tggatgcaga
     1261 tttatcttca ccggacgcag acttgtctat gatgtcgcgt catactattt ttcaacacgt
     1321 tgaaatcagg tcagggagag aagtatgaaa ttattgcagc ggggcgtggc gttagcgctg
     1381 ttaaccacat ttacactggc gagtgaaact gctctggcgt atgagcagga taaaacctac
     1441 aaaattacag ttctgcatac caatgatcat catgggcatt tttggcgcaa tgaatatggc
     1501 gaatatggtc tggcggcgca aaaaacgctg gtggatggta tccgcaaaga ggttgcggct
     1561 gaaggcggta gcgtgctgct actttccggt ggcgacatta acactggcgt gcccgagtct
     1621 gacttacagg atgccgaacc tgattttcgc ggtatgaatc tggtgggcta tgacgcgatg
     1681 gcgatcggta atcatgaatt tgataatccg ctcaccgtat tacgccagca ggaaaagtgg
     1741 gccaagttcc cgttgctttc cgcgaatatc taccagaaaa gtactggcga gcgcctgttt
     1801 aaaccgtggg cgctgtttaa gcgtcaggat ctgaaaattg ccgttattgg gctgacaacc
     1861 gatgacacag caaaaattgg taacccggaa tacttcactg atatcgaatt tcgtaagccc
     1921 gccgatgaag cgaagctggt gattcaggag ctgcaacaga cagaaaagcc agacattatt
     1981 atcgcggcga cccatatggg gcattacgat aatggtgagc acggctctaa cgcaccgggc
     2041 gatgtggaga tggcacgcgc gctgcctgcc ggatcgctgg cgatgatcgt cggtggtcac
     2101 tcgcaagatc cggtctgcat ggcggcagaa aacaaaaaac aggtcgatta cgtgccgggt
     2161 acgccatgca aaccagatca acaaaacggc atctggattg tgcaggcgca tgagtggggc
     2221 aaatacgtgg gacgggctga ttttgagttt cgtaatggcg aaatgaaaat ggttaactac
     2281 cagctgattc cggtgaacct gaagaagaaa gtgaccaggg aagacgggaa aagcgagcgc
     2341 gtgctgtaca cccctgaaat cgctgaaaac cagcaaatga tctcgctgtt atcgccgttc
     2401 cagaacaaag gcaaagcgca gttggaagtg aaaataggcg aaaccaatgg tcgtctggaa
     2461 ggcgatcgtg acaaagtgcg ttttgtacag accaatatgg ggcggttgat tctggcagcc
     2521 caaatggatc gcactggtgc cgactttgcg gtgatgagtg gaggaggaat tcgtgattct
     2581 atcgaagcag gcgatatcag ctataaaaac gtactgaaag tgcagccatt cggcaatgtg
     2641 gtggtgtatg ccgacatgat cggtaaagag gtgattgatt acctgaccgc cgtcgcacaa
     2701 atgaagccag attcaggtgc ctacccgcaa tttgccaacg ttagctttgt ggcgaaagac
     2761 ggcaaactca acgacctgaa aatcaaaggc gaaccggtcg atccggcgaa aacttaccgt
     2821 atggcgacat taaacttcaa tgccaccggc ggtgatggct atccgcgcct tgataacaaa
     2881 ccgggctatg tgaataccgg ctttattgat gccgaagtgc tgaaagcgta tatccagaaa
     2941 agctcgccgc tggatgtgag tgtttatgaa ccgaaaggtg aggtgagctg gcagtaatcc
     3001 gaaagtgccg gatatttgca tccggcacaa tgcttaatcg cggcgggcga tatcagcaaa
     3061 tttggcatcg aggatcttcg ccagatcgcc tgccgccagt tcgatatcca gtccgcgctt
     3121 gccgccagaa acataaatag tggcaaattc ttgtgcgggg gcgtcgataa tcgtcggcag
     3181 acgttttttc tgccccaatg ggctaatccc cccaaccagg tatcccgtcg aacgctgcgc
     3241 ggccatcgga tcggccatct caactttctt ggcacccagc gcttttgcta cttttttaag
     3301 atccagttga cctgcgaccg gcgtaacggc cactgccaga tgtttcatat caccgttcac
     3361 tgccaccagt agcgttttgt agacttgatc cggattcaaa cctaattttt tgacgacttc
     3421 atcgccaaaa ttggtttcag ccggatcgtg ctcgtaggta tggatttgaa acgaaatctt
     3481 gtttttttcg agtaatttaa ctgcgggtgt catggtaatt ttcctgcctt aaacaaaaaa
     3541 acgaagcaag catacgcctg atggcggtcc aaaaaaaaga gtcatcttgc ctaagagtat
     3601 tggcaggatg gtgagattga gcgacaatcg agttacaccg tcgcttaaag tgacggcata
     3661 ataataaaaa aatgaaattc ctctttgacg ggccaatagc gatattggcc atttttttag
     3721 cgcaacattt gcggcaaatt cccttctcca tacaggtgta gtgcaccgac cgcgaccaca
     3781 tatcgccccg gcggcatggc gcgtaattta tcccgccagg cgagatttcg ctgatgcatc
     3841 agcacatcgt acagcgactg actgaacgta ttgggcagcg ttatatcatt attttgcgga
     3901 ggtgcattca gccaccagct catcatttgt tgcagcaacc gtgcgttggt atgccagtgg
     3961 gtcagcgtat cgtccagcag cgccagtcct ttgtcaggga gctggagcaa catggtaatc
     4021 tggttttcag ccccttccag ttcaatcacg ggtttatgtt gttgcttcgc cgcctgcaat
     4081 aactggtaat cgataccgta ttctgcccgc agccccagtt tttgtgcctg cgtcgcctga
     4141 agaaccatcg cgatttgcca cagcggttga gtagaaaaga gcgacggaga aatgcccatc
     4201 tcctggctaa tgtgctgcag attttggagt tgctcctcgt taatgcgctc ttccagcgcc
     4261 tcgcaagtag gcaaattagc aaaaggcgta tcgctggtgg aaacatccgc ctcgacgatc
     4321 agcgcatcgg cgtttttgag ctttttgagc aaacgggtgg gcaggggagc catatcgtgg
     4381 ctacccatat gaatactgcc aatcagatga aaatggcgat tgccgggaag ggtgatatcg
     4441 atggcaggcc aggtgtaatg attaccgcgc agcgcggccc aaagcgtttt tacccggtac
     4501 aacagatcca tacgacctcc ctttgtgaaa tatcatgcta gcgcgcggtg agggatggcg
     4561 caaatggagt tggggacttg tttaatgtgt ttgtatgatt cagtatgttc ttgcatcgct
     4621 attcacaggg aagcaacagt taaaaaccat gaaacaggca acaagaaaac cgacgacacc
     4681 cggagatatt cttctctatg aatatctgga accgctcgat ttgaaaatca atgagttagc
     4741 agagttgctg catgttcatc gtaatagcgt cagtgcactg atcaataaca atcgtaaact
     4801 caccactgag atggcattcc gtctggcgaa agtttttgat accacagtcg attttaggct
     4861 aaacctccag gcggcagttg atctttggga agttgaaaac aacatgcgca cccaggaaga
     4921 attgggacgg attgaaacag tggctgaata tttggcacgc cgtgaagagc gtgcaaaaaa
     4981 ggtcgcgtaa ggccaaaaca tcgcttcatc tcgtaggccg gataaggcgt tcacgccgca
     5041 tccggcatcc gagcaccatt gcctgatgcg acgcttaccc gtcttatcag gcctacaaac
     5101 ctgtgcctga accgtaggcc ggataaggcg tttacgccgc atccggcagt catgcgtcga
     5161 tgccaaatgc gccgccctaa agcagcgcat ccgcaatgac gtacttattc cttcggttta
     5221 aaccgcagca accggttggc gttactcact acggtaatcg acgagagcgc cattgccgct
     5281 ccggcaacta ccgggttaag cagtgttccc gtaaacggcc acaaaatacc ggcggcgacc
     5341 ggaataccga tgctgttgta gataaaggca ccgagcaggt tctgcttcat gttgcgcagc
     5401 gttgcgcggg aaatcgcgag cgcatccgca acgcccatca ggctatggcg catcagggta
     5461 atcgccgcgg tttcaatggc aacatcactg ccgccaccca tcgcaatgcc gacatccgcc
     5521 tgggccagcg ctggcgcgtc gttaatgccg tcgcccacca ttgccacctg acgtccttca
     5581 ctttgcagac gtttgatcgc ttcggcttta ccgtccggca gcactccggc aatcacttca
     5641 tcaattccag cttctttggc gatcgcattg gcggtggttg ggttatcccc ggtcaacatc
     5701 accagacgat atcccgtttt atgcaggcgt tgcagcgccg ccacgctatc actacgcaac
     5761 ggatcgcgta cagccagcag ggctactgct ttcccgtcaa tcgccagcag cacaggcgtt
     5821 gctccttgcg atgcctgagc agtaatctcc gcttcgatag ctttggtacc aacctgttgc
     5881 tcatttaaca gcgcctgatt gcccagcaat aacgcatgac cttcagcttc accgctcacg
     5941 cccagcccgc gcaatgtgcg gaaaccgttg acctgcggca gctgcatatc acctgcttta
     6001 tcgaggatcg cccgtgccag cggatggctg gaaccttgct ccagtgccgc cgccagacgc
     6061 aatgcctgcg cttcatcaac atcagcaaac gttttcactg cgacaacctg cggcttccct
     6121 tcggtcagcg tcccggtttt atcgaacacg atggtatcga gtgtactggc gcgttgcagc
     6181 gcgtcggcgt cccgcaccag cacgccaaac tcagccgccc gcccgacgcc ggaaataatc
     6241 gacatcggcg tcgccagccc cagcgcacac ggacaggcga taatcagtac cgtggtggca
     6301 atcaccaggg tataaacaat ttgcggtgcc ggaccaaaga agtaccagat tgccgcactg
     6361 accagcgcaa tcaccaccac cacaggtaca aatacggctg agattttatc cgccagctga
     6421 ccgatttctg gcttgctgct ctgggcctgg cgcaccatgc gaatgatccg tgacagcgtg
     6481 gtatggctgc caaccgcact ggcacgaaac agcacactgc cgtcctgtac cactgtcccg
     6541 gcatggacgc tttcgccttc gcctttttgc tgtgggattg gttcgcccgt cagcatcgct
     6601 tcatccagcc atgcttcgcc ctgggtaatc tcgccatcta ccggcacgcg atcgccggtc
     6661 gtcaggcgca gtaacatccc tggctgaact tccgccagcg gcacgctttt ttcaccatct
     6721 gcggtgacca ctcgcgccgt cggtggggtt aaatcgagta atttctccag cgccttagaa
     6781 gaacgctgac gtgcgcgcgc ttccagcata tggccgagat taatcagacc gataatcatc
     6841 gcactggctt catagtaaag atgccgcgct tccatcggga accactgcgg ccacaggttg
     6901 acgctcatcg aatagagcca cgccacaccc gttcccagcg ccaccagcgt atccatcgtc
     6961 gctgcaccgt tcagcaggct tttccatgca ctgcggtaaa aatggccgcc ggcgaaaacc
     7021 atcactgcca gggttatcag gccgataact aaccacaagc tgcggttgtc agcggtgacc
     7081 atcatgttat cgccgatcat cccccagacc attaccggga tacccacagc cagtgcaaca
     7141 attgcctgcc agcggaagcg cttcatcgta gctacggcgg tttcttgctg gcgctcgcgg
     7201 cgtttagcgt catcttcaat cgcttccgca ccgtagcccg ctttttccac cgcctgcact
     7261 aaatcttgtg gggaggcact gcccatcacc agcgcagtac gctccgccag gtttacccgt
     7321 gcctgagtga cgcccggtac gctttgcagc gcattttgta cgcgggtgac acagctggcg
     7381 cagctcatgc cgctcagcag caactgctgg ctgtcatcgt catcggcggt cgctgccgga
     7441 agcgcctcag aaaccgctgt cagtgcttcc gacgggattg atgactccgc cagcggttta
     7501 gcctttgggt ggcttacaga tgcgtcataa cccgcttgtt tgatggtttc gatcagctgt
     7561 tctgcactgg cagtcccggt aacgtgcgct tcagtgatag acacatccgc ctgctcaaca
     7621 tccgggcgct gttcaagact ttctttcacg cgtttaacgc agtgaccgca ggacaggccg
     7681 tccagggtca ggtcgatagt ttgtgacata aaacactcct ttaagacagt tttgactggc
     7741 tgtgataaag gttaaacctt ccagcaaggg gaaggtcaag aaattaataa accaggcggg
     7801 taaaagtccg taaagattaa aaaatcggct cgatttgcat caggattaga catttatttc
     7861 tttgttttcc tgtagttaag ttgcgggtgc taagttaaat caggatgcct gaaaatcggc
     7921 accggggtga ggaattacct cccgcatcta taaaaaggag ttaacaaaag atgttagatg
     7981 caaacaaatt acagcaggca gtggatcagg cttacaccca atttcactca cttaacggcg
     8041 gacaaaatgc cgattacatt ccctttctgg cgaatgtacc aggtcaactg gcggcagtgg
     8101 ctatcgtgac ctgcgatggc aacgtctata gtgcgggtga cagtgattac cgctttgcac
     8161 tggaatccat ctctaaagtc tgtacgttag cccttgcgtt agaagatgtc ggcccgcagg
     8221 cggtacagga caaaattggc gctgacccga ccggattgcc ctttaactca gttatcgcct
     8281 tagagttgca tggcggcaaa ccgctgtcgc cactggtaaa tgctggcgct attgccacca
     8341 ccagcctgat taacgctgaa aatgttgaac aacgctggca gcgaatttta catatccaac
     8401 agcaactggc tggtgagcag gtagcgctct ctgacgaagt caaccagtcg gaacaaacaa
     8461 ccaacttcca taaccgggcc attgcctggc tgctgtactc cgccggatat ctctattgtg
     8521 atgcaatgga agcctgtgac gtgtacaccc gtcagtgctc tacgctcatc aatactgttg
     8581 aactggcaac gcttggcgcg acgctggcgg cgggtggtgt gaatccgttg acgcataaac
     8641 gcgttcttca ggccgacaac gtgccgtaca ttctggccga aatgatgatg gaagggctgt
     8701 atggtcgctc cggtgactgg gcgtatcgcg ttggtttacc gggcaaaagc ggtgtaggtg
     8761 gcggtattct ggcggtcgtc cctggcgtga tgggaattgc cgcgttctca ccaccgctgg
     8821 acgaagaagg caacagtgtt cgcggtcaaa aaatggtggc atcggtcgct aagcaactcg
     8881 gctataacgt gtttaagggc tgatcatgat gaacacggaa ggtaataacg gtaacaaacc
     8941 cctcggtcta tggaacgtcg tttccatcgg tattggggca atggtggggg cggggatctt
     9001 cgcgctgctg gggcaggctg cgttgctaat ggaagcctcg acctgggtcg cctttgcttt
     9061 tggcggtatt gtggcgatgt tttccggtta tgcctatgcg cgtctggggg cgagctatcc
     9121 cagcaatggc ggtattatcg acttttttcg tcgcggatta ggcaacggcg tcttttcgct
     9181 agcgctctcg ttgctgtacc tgttgacgct ggcggtgagc atcgccatgg ttgcccgtgc
     9241 ttttggcgct tatgccgtgc agtttttgca tgaaggcagc caggaggagc accttatttt
     9301 gctctacgcg ttggggatca ttgcggtgat gacgcttttt aactccttaa gcaaccatgc
     9361 ggtagggcgg ctggaagtga tcctcgtcgg cattaaaatg atgatcctgt tattgctgat
     9421 tattgccggt gtctggtcgc tgcaaccggc gcatatttcc gtctctgcgc cccccagctc
     9481 cggtgcgttc ttctcctgta ttgggataac cttccttgcc tatgcgggct ttggcatgat
     9541 ggcgaacgcg gcggataaag tgaaagatcc gcaggtcatt atgccacggg cgtttctggt
     9601 ggcgattggc gttaccacgt tgctttatat ctcgctggcg ctggttttgc ttagcgatgt
     9661 atcggcatta gagttagaaa aatatgctga taccgccgta gcgcaggctg cttctccgct
     9721 gctcgggcat gtgggttatg tgatcgtcgt catcggcgct ttactggcga cggcttcagc
     9781 cattaacgcg aacctgttcg ccgtgtttaa catcatggac aacatgggca gcgaacgcga
     9841 actgccgaag ctaatgaata aatccctgtg gcggcagagt acctggggca acattattgt
     9901 cgtggtgttg attatgctga tgacggcggc gctaaattta ggctcactcg ccagtgttgc
     9961 cagcgccacc tttttgattt gctacctggc ggtgtttgtg gtggcgatcc gcctgcgtca
    10021 tgatattcac gcctcgttgc cgattcttat cgttggtacg ttggtgatgt tgttggtgat
    10081 cgttggcttt atctacagtc tgtggtccca gggtagccgt gcgttgatat ggattattgg
    10141 ctcactctta ctcagcctta ttgtggcaat ggtcatgaag cgcaataaaa ccgtataaca
    10201 tctctctgtg cgcagtactt cctgtattat tgtggtggcg gtcgatattc gcactggcaa
    10261 aaaaacgtgc ttgaatatct gttgaaaccc tttaacaaag cacaggaggc gttgcgcgaa
    10321 cgatgaacat cagcgatgta gcaaaaatta ccggcctgac cagcaaagcc attcgcttct
    10381 atgaagagaa ggggctggtg acgccgccga tgcgcagcga aaacggttat cgcacctaca
    10441 cgcagcagca tctcaacgaa ctgaccttac tgcgccaggc acggcaggtg ggctttaacc
    10501 tggaagagag cggcgagctg gtgaatctgt ttaacgaccc gcagcggcac agcgccgacg
    10561 tcaaacggcg cacgctggag aaggtggcgg agatcgaacg acacattgag gagctgcaat
    10621 ccatgcgcga ccagctgctg gcactggcga atgcctgccc tggcgatgac agcgccgact
    10681 gcccgattat cgaaaatctc tccggctgct gtcatcatcg ggcagggtga ttaagacgag
    10741 acggctcgga tatgtagggt tatcccttct atcgcaatga cttcaacatg cgtacctgca
    10801 ccgagatcct cgctggcgct gacaggccat gaactgtcac cgacgcgcat atgaccgcgc
    10861 ccgttgacca gcggagattc cagcacaaaa cgtcggccaa tcagctgctg cccgcgctgg
    10921 tttaaatgac tatcgctgtg cttttgttcg cgcacccgcc gcgacaacca tttccaccac
    10981 agccaggcgg cgagcagcgt caggatggca aacattaccc cttgccactc ccaacccagc
    11041 ggcaccagcc agaccaccag gccggtaatc accgctgcca cgccgctcca caacaaataa
    11101 ccatttccgc ccagcatctc ggctgccagc agcaaaccgc cgagactgag ccagaaaata
    11161 tgtggatgaa cgaccattaa ctccatcatg gctgagtccg tttgttggcg ctgtctttca
    11221 ccagctcggc aatcccggca atcgacccca tcaggctgct ggcctctaat ggcatcatca
    11281 ctactttgct gttactggag gtaccgatct gctgtaacgc ttcggtgtat ttctgcgcta
    11341 cgaagtagtt caccgcctga atatcaccgg aggcgatagc ttcagacacc attttggtgg
    11401 cgcgggcttc tgcttcggcg gaacgttcac gcgcttcagc ctgtaaaaac gccgactgac
    11461 gttcgccttc cgctttcagg atttgcgact gtttttcacc ttcggctttg aggatttccg
    11521 cctgacggat cccttccgct tcaagaatgt aagcgcgttt ggtacgttcc gctttcatct
    11581 gcgcgttcat tgaagagata agctctgccg gtgggcgcac gtcgcgaatt tcaatacggg
    11641 tgactttaat cccccacggg ttggtggctt catcgacaat acgcagcagg cgtgagttga
    11701 tgctgtcgcg ctgagagagc atttcgtcaa gttccattga acccaacacg gtacggatgt
    11761 tagtcatggt caggttgatg atcgccagct ccagattgct gacttcataa gccgcgcgcg
    11821 gcgcgtcaat cacctgaata aaacagactg cgtcgatggt aacgttggcg ttatctttcg
    11881 agataacttc ctgggaaggg atatcgagca cttgctccat catattgatc ttgcgaccaa
    11941 tgcgatccat aaacggcacc accagactga gccccggctg taacgttttg gtatagcgac
    12001 caaaacgttc taccgtccat tgatagccct gcggcacgat tttgacgccc gcgccgacaa
    12061 tgaccagcgc gacaaaaatg agaatcggga taaagataag catcggaaaa acctcctgtt
    12121 gtaccgtcca taatcagcaa aattgctgct tgattaaaca aattatacct gattactgaa
    12181 agagagttcc cccttattcc tgcgaaggat aaactgtttt tagtaaaaat cagaaaaagg
    12241 gaacagcgat gcaggaaaat agtcctttgc ttcagctaca aaacgtagga tatctggcgg
    12301 gtgatgcgaa gattcttaat aacatcaatt tttcgctgcg tgctggcgaa tttaagttaa
    12361 ttaccggtcc ttctggttgt ggcaaaagta cgctgctaaa aatagttgct tcattgatca
    12421 gcccaaccag cggaacgtta ctgtttgaag gtgaggatgt cagcacacta aagccagaaa
    12481 tctaccgcca acaagtctct tactgcgccc agacaccgac gctgtttggc gatacggtat
    12541 acgataatct gatctttccc tggcagatcc gtaaccggca gcctgaccca gccatttttc
    12601 tcgattttct cgaacgcttc gccttgccgg acagcatttt gacgaaaaat atcgccgagc
    12661 tatctggtgg tgaaaaacaa cgcatctcac tgattcgtaa cctgcaattt atgccgaagg
    12721 ttttattgct ggatgaaatc accagtgcgc tggatgaaag taataaacat aacgttaatg
    12781 agatgatcca tcgttatgtg cgcgagcaaa atattgccgt gctgtgggtg acacacgata
    12841 aagacgaaat taatcatgcg gataaagtga ttacactgca accgcatgcc ggagaaatgc
    12901 aggaagcacg ctatgaactc gcataatatt actaacgaat cattagcact ggcattaatg
    12961 ctggtggtgg tggcaatctt aattagccat aaagaaaaac tggcgctgga gaaagatatt
    13021 ctctggagcg tcggacgagc gataattcag ctgattattg tcggctatgt gctgaagtat
    13081 attttcagcg tggatgatgc cagcctgaca ttattgatgg tgttatttat ctgctttaat
    13141 gcggcgtgga acgcgcaaaa acgcagtaaa tatattgcta aagcttttat ctcatcgttt
    13201 attgccatta cggtcggggc gggaattacc ctggcggtgc tgattctctc agggtcgatt
    13261 gaatttatcc cgatgcaggt gatccctatc gccgggatga ttgccggtaa cgccatggta
    13321 gcggtggggt tgtgttacaa caatttaggg caacgggtca ttagcgaaca gcaacagatt
    13381 caggagaaac tgagtcttgg tgcgacgccg aagcaggctt cagcgatatt gattcgcgac
    13441 agtattcgcg cggctttaat tccgacggtc gattcagcaa aaacggttgg cttagtgagt
    13501 ttaccaggaa tgatgtccgg gctgatattt gccgggattg atccggtgaa ggcgattaaa
    13561 tatcagatta tggtgacctt tatgctgctc tcaaccgcca gcttgtcgac cattattgcc
    13621 tgctatttaa cctatcgtaa gttttataat tcgcgccacc agttggtggt gacgcaattg
    13681 aagaagaaat gatgcgatgc cggatgcgca gcatcgcatc cggcgttgtg gttcatgtgc
    13741 cgatcaacgg aatatcaata caacaatgca tacagctggc ggcgatactt cgacgccagt
    13801 gcatcacccg tacccagcgc agcgaggatc tcctggaacg ttttacgcgt ctgaccgtct
    13861 gcggcggtga gatctttacg cagatgcccg aacagcaact ccagcgcctc ttcattgcgc
    13921 ccaacctgat gcagttgcag cgccagttgc gtcgccagtg cggcatcttc tggattctcc
    13981 gccacctgct gttgcaactg ttgaatttcc ggcgtatcag ccgcctgctt cagcagttcg
    14041 atttgcgcca ccatcccctg gtagcgggtg tcctgatcct gtaacggaat ggttttcagc
    14101 accgcttctg catcttcaga acggttcagc gcaatcagcg tttctgccaa cagcaggccg
    14161 atctccccgt tctgattcga caactgccag gcgtctttca gcaatggcag ggcatcggtg
    14221 aaattgcctt cctgcatcag ttgcatcgcc tgctgcgctt tcagctcttc ttcgcgcggc
    14281 agcactttat ccagcagggc gcggatcgcc tcttccggtt gcggcccctg gaagccatct
    14341 accggttgcc cgttctggaa cagatacacc gtcggaatcg cgcgcagacc aaactgggcg
    14401 gcaatcatct gctccgcatc gcagtccagc ttcgccagaa taaattgccc gttgtactgc
    14461 gccgcaaggc tttccagaat tggggttaac tgcaaacagt gctggctacg ttcagaccaa
    14521 aaatagaaca gcaccggagt ggtcatcgac tgttcaagaa cctgttgcag gttagattcg
    14581 ttaatgttga caatattttc tacggacatg gagtcgctct ctgttgtcga tattttcttt
    14641 gacatggggg cttaagcgcg cgcttcaact cacccctgca atattttgtc catcacgcgc
    14701 cccggcagca ggcgcttaag caccattact gcccaagtca ccagcgttac cggatagcgc
    14761 atcttcggct tctcgctaat aaaagcatgg cgtactttgt ccaccaccgc ttccggtccc
    14821 aacgtaaagc gggcggcgat gccgggattt tcgactggtt tatcactttg cgtctggttg
    14881 acgttgtcgg tgaagcgagt acgaatggga ccgggttcga tcaggctgac tttaattccg
    14941 ctgtggcgta gctccatgcg cagcgcgtct gaccatgcct ccagcgcata tttactggcc
    15001 gcgtaagcgc cacgacccgg tgtggagatt aatcccatca ccgatgatgt catcacaata
    15061 cgcccttcac cgtgcggtaa catcgcgggt aacaggcgca tggtgagctg gtgtgcgccg
    15121 aaaaagttgg cggaaaactg ctgttccatc tgcgcgcggc tgatggtgga aagggggcca
    15181 tacatgccga atccggcatt gttaaagatc ccatacagac aattatcggt cagggcgatc
    15241 acttcgtcgg ctgcgcgatc aacactttct ggcgaatcca gatcgatcaa cacgccggta
    15301 aatcccatgc tgttcatacg ctcaacatca tccggtttcc ggcaacctgc cagcacatga
    15361 aaaccctggc gttttaattc gagcgcgctt tccaggccaa ttccactgga acatccggta
    15421 attaagaccg atttttgcat aactttacct gtcaggatct ccgttgcttt atgagtcatg
    15481 atttactaaa ggctgcaact gcttcgccat ccagtcggca ataaacggct gggcgtcgcg
    15541 gttgggatga ataccgtcat cctgcatcca ttgtggcttg aggtagacct cttccataaa
    15601 aaagggcagc agcggaacat caaactcttt ggcgagtttg gggtaaatgg cgctaaaggc
    15661 ttcattataa cggcgaccat agtttgcagg cagacgtatt tgcattaaca atggttcagc
    15721 gttggcggct ttgacatcct gcaaaatctg gcgcagcgtt tgctcggttt gctgtggctg
    15781 aaaaccacgc aaaccgtcat tgccgcccag ttcaaccagc acccaacgcg gctgatgctg
    15841 tttcagcaga gccggaaggc gcgccagtcc ttgttgcgag gtgtcgccgc tgatgctggc
    15901 attaactacc gacgttttac tctgccactt atcattcaac aaggcaggcc aggccgcgct
    15961 ggcagacatt cgatacccgg cgctcaggct atcacccaga atcaataacg tgtccgctgc
    16021 ggcggcacgg aaggttaaca gggccaggaa caggaagggc aaatgccagc ggaaaacatt
    16081 gttgaagttc atcatcttaa gaagtccgtc ggtcaggggg agcatgaact ctccatcctc
    16141 accggagttg agctggttgt caaacgtggc gagaccatcg cactggtggg cgagtcggga
    16201 tcgggtaagt caaccttgct ggcgatcctc gccgggcttg atgacggcag cagtggcgaa
    16261 gtgagtctgg tgggacaacc gctacataat atggacgaag aagcgcgggc aaagttgcgc
    16321 gcgaagcacg tcggctttgt ttttcagtca tttatgttaa ttcctaccct taacgcgctg
    16381 gaaaacgtcg agcttccggc tctgctgcgc ggtgagagta gcgcggaaag tcgtaacggg
    16441 gcgaaagcgt tgctcgaaca gttagggctg ggtaaacgtc tggatcatct tccggcacag
    16501 ctttccggcg gtgaacagca acgagtggcg ctggcacgag cctttaatgg tcgacctgat
    16561 gtgctgtttg ccgacgaacc caccggcaac cttgaccgcc agacgggcga taaaattgcc
    16621 gacctgctgt tttccctcaa ccgtgaacat ggcaccacgt tgattatggt gacccacgac
    16681 ctgcaactgg cggcacgctg cgaccgctgc ttacggctgg tgaacgggca gttgcaggag
    16741 gaagcatgat tgcacgttgg ttctggcgcg aatggcgttc gccgtcgcta ttaattgtct
    16801 ggctggcgct aagcctggcg gtggcctgcg tgctggcgct gggcaatatc agcgatcgca
    16861 tggagaaggg cttaagccag caaagccgtg agtttatggc gggcgatcgg gcgttgcgca
    16921 gttcacgcga agtgccgcaa gcgtggctgg aggaagcgca aaagcgcggc ctgaaagtcg
    16981 gcaagcagct gactttcgcc acaatgacct ttgcaggcga cacaccgcag ctggcgaacg
    17041 tcaaagcggt ggatgatatc tacccgatgt atggcgatct gcaaactaat ccccctggcc
    17101 tgaaaccgca ggcgggcagc gtattgctgg ccccacgcct gatggcactg cttaacctga
    17161 aaacgggcga caccattgac gtgggcgatg ccaccttgcg gattgccgga gaagtgattc
    17221 aggaaccgga ttccggtttt aaccccttcc agatggctcc gcgtctgatg atgaatctgg
    17281 cggatgtcga taaaaccgga gccgtgcagc cggggagtcg ggtcacctgg cgttataaat
    17341 tcggcggcaa cgagaaccag ctcgacggct atgataaatg gttgttacct cagcttaaac
    17401 ccgaacaacg ctggtacggt ctggaacagg acgaaggcgc gctggggcga tcgatggaac
    17461 gctcgcaaca gttcctgctg ctttcggcgc ttctgacctt gctgctggca gtggcagcgg
    17521 tggcggtagc gatgaatcat tactgtcgca gtcgctacga tctggtggcg atcctcaaaa
    17581 cgctgggggc agggcgagcg caactgcgta agctaatcgt cggtcagtgg ttgatggtgc
    17641 tgacgctttc agccgttacc ggtggggcca taggcctgtt gttcgaaaac gtgttgatgg
    17701 tgctgctcaa gcccgttctg cctgctgcac taccgccagc cagcctctgg ccgtggctgt
    17761 gggcgcttgg caccatgacg gtcatctcgc tgctggtggg gctacgacca taccgcttgt
    17821 tgctggcaac gcagccttta cgcgtattac gtaatgatgt ggtagcgaac gtctggccgc
    17881 tgaagtttta tctgccgatt gtcagtgtgg tggttgtgct gctgctcgcc ggattaatgg
    17941 gtggcagcat gctgctttgg gcggtgctgg cgggcgcggt agtactggct ttgctgtgcg
    18001 gtgtgctggg ctggatgcag ctgaatgtac ttcgccgcat gacgctgaaa tcgctacctc
    18061 tgcgcctggc ggttagccgc ctgttacgtc agccgtggtc aacgttaagc cagctttcgg
    18121 cattttcgct ctcctttatg ctgctggcgc tgttgctggt attgcgtggc gatctgctcg
    18181 accgctggca gcagcagcta ccgccagaaa gcccgaacta ctttttaatt aacatcgcca
    18241 cagaacaggt agcaccgcta aaagcgttcc tcgcggaaca tcatatagtc ccggaatcat
    18301 tttatccggt agtgcgggcg cggctgacgg cgattaacga taagccgaca gaaggtaatg
    18361 aagatgaggc gcttaatcgc gaactcaatc ttacctggca aaatacgcgg cccgatcata
    18421 atccgattgt cgccggtaac tggccgccaa aagccgatga agtgtcgatg gaagaggggc
    18481 tggcgaaacg cttaaacgtt gccctcggtg ataccgtgac ttttatgggc gatacccagg
    18541 agttccgcgc taaagtgacc agcctgcgca aagtggactg ggaaagtctg cggcctaatt
    18601 tctattttat tttcccggaa ggggcattag acgggcaacc gcagagctgg cttaccagtt
    18661 tccgctggga gaatggcaac ggcatgttga cgcaactcaa ccgccagttc ccgaccatta
    18721 gcctgttaga tattggcgcg attttaaaac aggtcggtca ggtgctggag caggtaagtc
    18781 gggcgctgga ggtgatggtg gtgctggtca ccgcctgcgg tatgttgctg ttgctggcac
    18841 aggtgcaggt gggaatgcgt cagcgtcatc aggagctggt ggtgtggcgc acactcggtg
    18901 cggggaaaaa actgctgcgt accacgttgt ggtgtgagtt cgccatgctt gggtttgttt
    18961 ctggcctggt agccgcaatt ggtgcggaaa cggcgctggc agtgttgcag gcgaaagtgt
    19021 ttgatttccc gtgggagcca gactggcgat tgtggattgt tctgccgtgc agcggtgcac
    19081 tgctgctgtc gcttttcggc ggctggctgg gtgcgcgact ggttaagggt aaggcgctgt
    19141 tcaggcagtt tgcggggtga tgacaggtgt tagattgcct gatgcgctac gcttatcagg
    19201 cctacgaaga gcgtgcaata tattgatttt gcaagattgt gtaggccgga taaggcgttt
    19261 acgccgcatc tggcatgaac agtgtgcatt ttggcaataa atatgaaacc aactgtcatg
    19321 tcggtttttt atatctgtat tctggtgcaa ccattctgtt ctcacgacaa tcgccgtttc
    19381 agaataccgc ccatcactgc gtcttaaccc attccgccac ttccgcccac tcaccgcgaa
    19441 agacaacttt ttccgctttt ttctcaagct gatagcgata catcgggtcg taatattctt
    19501 taagtaacgg caccagccag gccagatgac cgtcggtgct gtcggtggtg agttgcgttg
    19561 tcagcgccgt atccagctgg gcagcgagtt cgttatagcg ctgtaacccc agccgacgct
    19621 taatagccga aagtccgtga tgcaggtatt cgcaatattc ctgccagccc tgttcgtcgc
    19681 cgtacgcgtg ggtaaaatca tgatgcatac gcaagaaata ctcttcgttc aggcgctcaa
    19741 gacggatctc aaacggatct tctaccaccg caatcgccgc ctgagtcatt cgctcgcgca
    19801 ggcattccgg caggtgattc gaaccgatca tccggctttc gtcttccagc acccacaggc
    19861 gcaaatcctg acgggcgtcg gtttttagca tttcggcagc cagcaggttt tcaaaactcg
    19921 cctggctaag ttgtggttgt aaggtgcgac caaacgccga accgcgatga cgcgctaacc
    19981 cttccagatc aacaccgttc ggctgttgct gcactaacag cgttttaccg ctgccggtac
    20041 aaccgccaat cagcactatc ggtttttgtg ccagttcaat agtcgcctga atcgcggctt
    20101 ggcgcagtgc cttataaccg ccttccacca gcggataatc aatccccgct gcatacaacc
    20161 agctttgcac aatatgtgag cgctgaccgc cacgggcgca gcagagaata ccttgcggat
    20221 tttgcaggca cgctgcccgc caggcgtcca tgcgctgctg acgaatttca cccgccacca
    20281 gtttatgccc cagcgccagc gccgcgtctg agccttgctg tttataacaa gtgccaacgg
    20341 cggcgcgttc atcgttattc attaacggca ggttgatagc ggcgggcatt gcgccttgct
    20401 caaactcgat aggggcgcga acatcaataa tgggcgtatc agcaatcagc agggcacgat
    20461 agtcctgttc cgtgtgtctc tcttgcatag ttaaaagtga acctcaaatc agcttgcgcg
    20521 ctattttacg cgccaacgcg caaggaaact tgatttttaa ctgcgtgggt tgccgaaaat
    20581 ttctaaaaat ccgctgattt ccggcctgcg ctgggtaaac aaggtcacaa tatcttctac
    20641 cgctttgccg ctgccaaatt tgcgccatgc cagactcaat ggcgaaggag ggcgcatcgt
    20701 tgggattacc cggctgacca gttgttgatt atcgatcatt gactggcaaa gtgattttgg
    20761 caaaaaacca atgccaacac ccgccagatg ggcggcgatt ttcgtttcca tatcgggaac
    20821 aataatctct ttttgccccg gcaatcgcca ggcgacgcgt ttggttaagg ttcgggcgct
    20881 gtcttcaata ttgaccgccg gaaagcgccg caactgcgct tctgttagcg gctcttcaac
    20941 gttcgccagc ggatgatccg ccgccatgac aaagcgccat tgcaccgatc ctaagggatc
    21001 aagactaaag gtatttgcca gcgcctcagt tcccgtgacc ccgatagcca gcgaaaaacc
    21061 ttcgtacaat agcgagtccc agacgcccat atagatttgt cgggagatgt gaaactgggt
    21121 aaaggggtaa cgctcattca gccacgccag caactgggcg acggcctggg ggttatagag
    21181 caggttgttg atgacaatat tcacctagcg ttccacgcca tcattcacct gttgcagctc
    21241 gctgggcata ctttccagcc agctcagcca gtctctggcc tgggaaagta gatgctcgcc
    21301 agccgctgtc aacgtcacgc tgcgagtcgt gcggaaaaac agcgccactc cggtattctc
    21361 ttccagaagt ttaatgcgat aactgatcgt cgccgtggtt ttacataatc gttctgccgc
    21421 ttttgaaaaa cttcctgttt cagcaaccgc aatgaaagtc cgcaaggttt ctggatcgaa
    21481 catcttcagg tatccccttt taaatccgca agttgcgtga ttttcttatc ctctgattta
    21541 tcagtatttt tacatgataa ccctgttcaa tttgtggact aaatctagtt ttggaaaaat
    21601 attccaactt ttgtattgat gttgttctct taaggtttta gat
//
LOCUS       DAEEKQ010000058        21455 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.058, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000058 DAEEKQ010000000
VERSION     DAEEKQ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21455
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.058"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(331..876)
                     /gene="hemG"
                     /locus_tag="KLK57_002095"
     CDS             complement(331..876)
                     /gene="hemG"
                     /locus_tag="KLK57_002095"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HBE0892460.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAQLTNKPTLK"
     gene            complement(888..2339)
                     /gene="trkH"
                     /locus_tag="KLK57_002096"
     CDS             complement(888..2339)
                     /gene="trkH"
                     /locus_tag="KLK57_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HBE0892461.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            complement(2378..2992)
                     /locus_tag="KLK57_002097"
     CDS             complement(2378..2992)
                     /locus_tag="KLK57_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HBE0892462.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITSVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYHQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="KLK57_002098"
     CDS             complement(2992..4323)
                     /gene="pepQ"
                     /locus_tag="KLK57_002098"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HBE0892463.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHASVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            4513..6702
                     /gene="fadB"
                     /locus_tag="KLK57_002099"
     CDS             4513..6702
                     /gene="fadB"
                     /locus_tag="KLK57_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HBE0892464.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            6712..7875
                     /gene="fadA"
                     /locus_tag="KLK57_002100"
     CDS             6712..7875
                     /gene="fadA"
                     /locus_tag="KLK57_002100"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HBE0892465.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQAGLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRARELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMEHKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(8065..8766)
                     /gene="fre"
                     /locus_tag="KLK57_002101"
     CDS             complement(8065..8766)
                     /gene="fre"
                     /locus_tag="KLK57_002101"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HBE0892466.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="KLK57_002102"
     CDS             complement(8813..10306)
                     /gene="ubiD"
                     /locus_tag="KLK57_002102"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HBE0892467.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            10473..10961
                     /gene="rfaH"
                     /locus_tag="KLK57_002103"
     CDS             10473..10961
                     /gene="rfaH"
                     /locus_tag="KLK57_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HBE0892468.1"
                     /translation="MQSWYLLYYKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASLAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="KLK57_002104"
     CDS             complement(10958..11740)
                     /gene="tatD"
                     /locus_tag="KLK57_002104"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HBE0892469.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVAHGIYIGITGWVYDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTRFGIAF"
     gene            complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="KLK57_002105"
     CDS             complement(11782..12558)
                     /gene="tatC"
                     /locus_tag="KLK57_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HBE0892470.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="KLK57_002106"
     CDS             complement(12561..13076)
                     /gene="tatB"
                     /locus_tag="KLK57_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HBE0892471.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="KLK57_002107"
     CDS             complement(13080..13349)
                     /gene="tatA"
                     /locus_tag="KLK57_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HBE0892472.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="KLK57_002108"
     CDS             complement(13428..15068)
                     /gene="ubiB"
                     /locus_tag="KLK57_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HBE0892473.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVATLEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="KLK57_002109"
     CDS             complement(15065..15670)
                     /gene="ubiJ"
                     /locus_tag="KLK57_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HBE0892474.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="KLK57_002110"
     CDS             complement(15684..16439)
                     /gene="ubiE"
                     /locus_tag="KLK57_002110"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HBE0892475.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="KLK57_002111"
     CDS             complement(16534..17961)
                     /gene="rmuC"
                     /locus_tag="KLK57_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HBE0892476.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQNEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            complement(18102..18863)
                     /gene="udp"
                     /locus_tag="KLK57_002112"
     CDS             complement(18102..18863)
                     /gene="udp"
                     /locus_tag="KLK57_002112"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HBE0892477.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGIMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            19125..19937
                     /locus_tag="KLK57_002113"
     CDS             19125..19937
                     /locus_tag="KLK57_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HBE0892478.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTLDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHAASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPILGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGEKRS"
     gene            20331..21392
                     /gene="hcp"
                     /locus_tag="KLK57_002114"
                     /pseudo
     CDS             20331..21392
                     /gene="hcp"
                     /locus_tag="KLK57_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278761.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
BASE COUNT         5265 a         5740 c         5376 g         5074 t
ORIGIN      
        1 cactcttgag acttggtatt catttttcgt cttgcgacgt taagaatccg tatcttcgag
       61 tgcccacaca gattgtctga taaattgtta aagagcagtg ccgcttcgct ttttctcagc
      121 ggcgcggggt gtgcataata cgctttcccg ctacagagtc aagcatttat ttttgctttc
      181 tctgtcggaa ttctcaggag aaccccgctg acccggcggc gtgtttgccg ttgttccgtg
      241 tcagtggtgg cgcattatag ggagttattc cggcctgaca agaggaaatt taaaataatt
      301 ttctgaccgc gcatttttta ttctttatgc ttatttcagc gtcggtttgt tggttaactg
      361 ggcgatttct cgggcgaaat tcgccacctg ctcccaatcg gtatagacaa cttctttgcg
      421 cgtatccgtt tcaccgcctg acatcttcat aattagcttg atcataaaac gatcgtacca
      481 gcgataacgt gggtaacgca gcgccccggc aatgaccgcg cagcgatcgg gacgccattg
      541 cgagttcatc agaaacttcc gcgcgtagct gttggtctgt ggagtacgct tctccggttt
      601 gcgcgccacc aggtttacgg aataaaaggc gctcggcatc gaattcagtc gcgtcgcatg
      661 ttttttgaca aattcctgga acgcagagtg gtagtgacca tagcgaatag aagcaccaat
      721 gaccacacgg tcataatttt cccattgtgg ttcttcaatg cggtgcacat tggcgacatc
      781 cgtctggatc cccagttctt tcagttccga agccaggtag gaggcaatct cgcgcgtttg
      841 tccgtccctt gttgagaaaa gaattaatgt tttcacgtat tactccatca ttcacgccag
      901 aaagtcgggg taaagagcac cagcaatgta aagacctcga gacgaccaaa cagcatgttg
      961 gcaatcagga tccatttagc caccgggttc atactggtaa agttatcagc aaccacgcca
     1021 agccctggcc ccaggttatt caatgtcgca acaactgaag caaaggcaga aaagtcatcc
     1081 acgcccgtgg cgataatcgc cagcatactg acaataaaca ccaatgcata ggcggagaaa
     1141 aatccccaaa cggcttcgag gatacgttcc ggcagtgcgc gattccccag cttaatgcta
     1201 tagacggcgt tcggatgcac cagtcgtttc agctcacggt tcccctgctt aaacagcagc
     1261 aggatgcgga tcactttcag gccaccgccc gttgacccgg cgcaaccgcc gataaaagct
     1321 gaacataaaa gcagtaccgg caaaaagagc ggccagcggg caatgctgtc agtcgtaaac
     1381 ccggcggttg tcgccatcga taccacctgg aaaaacgcct ggttaattgt catcagcgcc
     1441 gaactgtaga cattatgaaa ccacagtacg agtgtacaaa taaccaccag cgtaaactgt
     1501 acgccgataa acatgcgaaa ttccggatcg cgccaataaa ccttcagact acgcccactt
     1561 aacagtgaaa agtgcagacc gtagttacag ccggagatca gcaggaagat agcaatgata
     1621 gtgttaatag tcgggctgtc gaaataaccg atactggcat catgtgtcga gaagccgcca
     1681 atagcgatag tcgcaaagct atggccgatg gcatcaaagg catccattcc ggcaaaccac
     1741 aacgccagcg cacaggcgac ggtcagcaag acataaatca accacagggt tttcgccgtt
     1801 tccgcaattc gcgggcgcat tttgttatct ttcagcgggc cgggcatttc tgcgcgatag
     1861 agctgcatcc cacccacgcc gaggataggc agtatcgcaa ccgctaacac gatgatcccc
     1921 atcccgccaa accattgcag catctggcga taaaagagga tggcatgagg gagcgaatcc
     1981 agccccacca gcgtagtggc cccggtggtg gtcaggccag agaaagattc aaaaaacgca
     2041 tcggtaatcg tgaggttcgg gctttccgag aagataaaag ggagcgcacc gacactaccc
     2101 agcacggtcc agaacagcac cactatcaga aacccctcac gggatttcag ttcgcccttc
     2161 tctttacggt tcggccacca cagcatagaa ccaatggcga gggcgacaaa aaaggtctgg
     2221 gtaaaagcgc ggcccgctcc atcccggtag atgagtgcta ccagcccagg gataatcatg
     2281 gtccctgaaa ataagatgac cagtagtcca acgattcggg taatggcgcg aaaatgcatc
     2341 tctgccgctt ccttagttct gcaaaacgaa gtggggatta ttcttcaatc gctaacaatt
     2401 gcaatgaacc acggctaaaa tccgccagct ttgcggaaaa ttcagccact ttcgccgccg
     2461 gaagcgccac ccgcagcaga acgaatgcct gataatcact gttgataatt ttgccgtcac
     2521 actgccccag caacgcttca atgccggtta actgatgata ttcacattgc aaagtatatt
     2581 cggttaatgg cgtcttgcgt tgggtcgtta gctggcgcag cgcctgattc acgccgccgc
     2641 catacgcttt cactaaccca ccggtgccaa gcaatatgcc gccgtagtag cgcactacca
     2701 cagaggtaat ttccccgacg ccgctgccca ttagctgggc gagcatcggt ttacctgccg
     2761 ttcctgccgg ctccccgtcg tcagagaaac ccagctgttg agaatcatcc ggcgcacccg
     2821 ccacccacgc cacgcaatgg tggcgggcat cggggtgttc tgcccgcacc gattcaacaa
     2881 acgctttcgc cgcctcaacg ccatcggtat gcgccaacat cgttatgaag cggctttttt
     2941 tgatctcttc aacgaccgtg accggtgccg caggaattaa ccagctttcc atcacgccag
     3001 tttcagatcc cgggtcatgt tttccacgtt gttttcgtgg atcaccacgt tgtcttcgat
     3061 acgaatgccg ccgaacggtt tcagtgcttc aattttctgc cagttgaagt gcttgctgaa
     3121 ctgcccttca cgccacggtg ccagtagcga ttcaatgaag tagatacccg gttcgatggt
     3181 taacaccata cccggctgga gaatacgggt gcagcgcagg tacggatatt ttgccggtgc
     3241 cgcgaggtgt gtaccgctat catcctgcat aaaaccggct acgtcatgca cctgcaggcc
     3301 cagcggatga ccgataccat gcggcataaa cggtccggtg agatcgtttt cgaccatcgc
     3361 ttcttcactc atatcggtga tgatttgatg tttacgcagc agtttggcga tgcgctgatg
     3421 gaactggatg tggtaatcca catagctaac gcctgctttc atggtggcga tcagcgccag
     3481 ttgttcatca tttacgtctt tcaccagctg tgcgtagtcg ttgtcgcttt ttgccgacca
     3541 ggtacgagtc aggtcagccg catagccgtt atattcggcc ccggcatcca gcaggaagct
     3601 gcgcatctct tccggtgcct gatgatccag tttggtgtaa tgcagcaccg aagcgtgttc
     3661 gttaagcgcc acaatgttgc tgtaaggtac gtcggtatca cgatgaccgg tcgcggtcag
     3721 ataggcgatg ttgatatcga actcgctcat gccagaacgg aacgcttctt ctgccgcacg
     3781 atgaccgttg accgccattt tctgcgcttc acgcatacag gccagttcgt actcggtttt
     3841 gaaggagcgg tagtaatgca ggtagtcgat cacccctttc gggttgatgt tgctggcctc
     3901 aatacccagt tgcagcgcac gttccggcac cggaccgata taaccgatat tgccgcgcgc
     3961 agcgggcagc aggctaccaa tgccatcggc tttcggcagt gcgatcactt ccacatcttc
     4021 agtccagaag gaggtcggca gcggttcgac gttgtgccag taatcaaccg gcagatagaa
     4081 ccacagtttc ggcttgttca cgccatccac cagcaaccag cagtttggca cctgagttac
     4141 cggcacccac gctttgaatt gcgggttcac tttaaacgga tagggatgat cgtcgagaaa
     4201 aacgttgaac agctcgccgg agtgaataag taatgcatcc agcttgaagc gcgccagcgc
     4261 atcgcgagtc cgttcttgta aggtagctat atgattttta tagagcgagg ccagtgattc
     4321 cattttttac ccttctgttt ttttgacctt aagtctccgc atcttagcac atcgttcatc
     4381 cagagcgtga tttctgccga gcgtgatcag atcggcattt ctttaatctt ttatttgcat
     4441 atttttaaca caaaatacac acttcgatgc atctggtacg accagatcac cttgcggatt
     4501 caggagactg acatgcttta caaaggcgac accctgtacc ttgactggct ggaagatggc
     4561 attgccgaac tggtatttga tgccccaggt tcagttaata aactcgacac tgcgaccgtc
     4621 gccagcctcg gcgaggccat cggcgtgctg gaacagcaat cagatctaaa agggctgctg
     4681 ctgcgttcga acaaagcagc ctttatcgtc ggtgctgata tcaccgaatt tttgtccctg
     4741 ttcctcgttc ctgaagaaca gttaagtcag tggctgcact ttgccaatag cgtgtttaat
     4801 cgcctggaag atctgccggt gccgaccatt gctgccgtca acggctatgc gctgggtggt
     4861 ggctgcgaat gcgtgctggc gaccgattat cgtctggcga cgccggatct gcgcatcggt
     4921 ctgccggaaa ccaaactggg catcatgccg ggctttggcg gttctgtacg tatgccacgt
     4981 atgctgggcg ctgacagtgc gctggaaatc attgccgccg gtaaagatgt cggcgcggat
     5041 caggcgctga aaatcggtct ggtggatggg gtagtcaaag cagaaaaact ggttgaaggc
     5101 gcaaaggcgg ttttacgcca ggccattaac ggcgacctcg actggaaagc gaaacgccag
     5161 ccgaagctgg aaccgctaaa actgagcaag attgaagcca ccatgagctt caccatcgct
     5221 aaaggaatgg tcgcgcaaac ggcggggaaa cattatccgg cccccatcac cgcagtaaaa
     5281 accattgaag ctgcggcccg ttttggtcgt gaagaagcct taaatctgga aaacaaaagt
     5341 tttgtcccgc tggcgcatac caacgaagct cgcgcactgg tcggcatttt ccttaacgat
     5401 caatatgtaa aaggcaaagc gaagaaactc accaaagacg ttgaaacccc gaaacaggct
     5461 gctgtgctgg gcgcaggcat catgggtggc ggtatcgctt accagtccgc gtggaaaggc
     5521 gtgccggttg tcatgaaaga tatcaacgac aagtcgttaa ccctcggcat gaccgaagcc
     5581 gcgaaactgc tgaacaagca gcttgagcgc ggcaagatcg atggtctgaa actggctggc
     5641 gtgatctcca cgatccaccc aacgctcgac tacgccggat ttgaccgcgt ggatattgtg
     5701 gtagaagcgg ttgttgaaaa cccgaaagtg aaaaaagccg tactggcaga aaccgaacaa
     5761 aaagtacgcc aggataccgt gctggcgtct aacacttcaa ccattcctat cagcgaactg
     5821 gccaacgcgc tggaacgccc ggaaaacttc tgcgggatgc acttctttaa cccggtccac
     5881 cgaatgccgt tggtagaaat tattcgcggc gagaaaagct ccgacgaaac catcgcgaaa
     5941 gttgtcgcct gggcgagcaa gatgggcaag acgccgattg tggttaacga ctgccccggc
     6001 ttctttgtta accgcgtgct gttcccgtat ttcgccggtt tcagccagct gctgcgcgac
     6061 ggcgcggatt tccgcaagat cgacaaagtg atggaaaaac agtttggctg gccgatgggc
     6121 ccggcatatc tgctggacgt tgtgggcatt gataccgcgc atcacgctca ggctgtcatg
     6181 gcagcaggct tcccgcagcg gatgcaaaaa gattaccgcg atgccatcga cgcgctgttt
     6241 gatgccaacc gctttggtca gaagaacggc ctcggtttct ggcgttataa agaagacagc
     6301 aaaggtaagc cgaagaaaga agaagacgcc gccgttgaag acctgctggc agaagtgagc
     6361 cagccgaagc gcgatttcag cgaagaagag attatcgccc gcatgatgat cccgatggtc
     6421 aacgaagtgg tgcgctgtct ggaagagggc attatcgcca ctccggcgga agcggatatg
     6481 gcgctggtct acggcctggg cttccctccg ttccacggcg gcgcgttccg ctggctggac
     6541 accctcggta gcgcaaaata cctcgatatg gcacagcaat atcagcacct cggcccgctg
     6601 tatgaagtgc cggaaggtct gcgtaataaa gcgcgtcata acgaaccgta ctatcctccg
     6661 gttgagccag cccgtccggt tggcgacctg aaaacggctt aaggagtcac aatggaacag
     6721 gttgtcattg tcgatgcaat tcgcaccccg atgggccgtt cgaagggcgg tgcttttcgt
     6781 aacgtgcgtg cagaagatct ctccgctcat ttaatgcgta gcctgctggc gcgtaacccg
     6841 gcgctggaag cggcggccct cgacgatatt tactggggtt gtgtgcagca gacgctggag
     6901 cagggtttta atatcgcccg taacgcggcg ctgctggcag aagtgccgca ctctgtcccg
     6961 gcggttaccg tcaatcgctt gtgtggttca tccatgcagg cactgcatga cgcagcacga
     7021 atgatcatga ctggcgatgc gcaggcaggt ctggttggcg gcgtggagca tatgggccat
     7081 gtgccgatga gtcacggcgt cgattttcac cccggcctga gccgcaatgt cgccaaagcg
     7141 gcgggcatga tgggcttaac ggcagaaatg ctggcgcgta tgcacggtat cagccgtgaa
     7201 atgcaggatg cctttgccgc gcggtcacac gcccgcgcct gggccgccac gcagtcgggc
     7261 gcatttaaaa atgaaatcat cccgaccggt ggtcacgatg ccgacggcgt cctgaagcag
     7321 tttaattacg acgaagtgat tcgcccggaa accaccgtgg aagccctcgc cacgctgcgt
     7381 ccggcgtttg atccagtaag cggtacggta acggcgggta catcttctgc actttctgat
     7441 ggtgccgcgg ccatgctggt gatgagtgaa agccgcgccc gtgaattagg tcttaagccg
     7501 cgcgcccgtg tgcgttcgat ggcggtcgtt ggttgtgatc cgtcgattat gggttacggc
     7561 ccggttcctg cctcgaagct ggcgctgaaa aaagcggggc tttctgccag cgatatcggc
     7621 gtgtttgaga tgaacgaagc ctttgccgcg cagatcctgc cgtgcattaa agatctcggg
     7681 ctaatggagc agattgacga gaagatcaac ctcaatggtg gtgcgatcgc gctgggtcat
     7741 ccgctgggtt gttccggtgc gcgtatcagc accacgttgc tgaatctgat ggaacacaaa
     7801 gacgttcagt ttggtctggc gacgatgtgt atcggtctgg gtcagggtat tgcgacggtg
     7861 tttgagcggg tttaaccact tgccggatgc ggcgtgaacg ccttatccgg tctaccgatc
     7921 ctgcaccaat gtaggcctga taagacgcgc cagcgtcgca tcaggcatcg tgcaccgatt
     7981 gccggatgcg acatagatct aaatgccact gaataaagtt ttagttgccg ttcttcccgc
     8041 ctgtcagggg cgggttttta tatctcagat aaatgcaaac gcatcgccaa acagacgatc
     8101 ttcccgcgca ttacgctcac tgcaaaacag gtcacgagca attttcgcca tctcaaaacg
     8161 tccggcaata tagatatcat gctctgccag cgtaccatga tcctgcaaga ccgccgttaa
     8221 cacggtgcca gtacgcccac gccagcccgc ttccggttgt tcaaccaccg gcaccacttg
     8281 cagaccagga tgcttcagcg aaagcgcctc aagctcgcag agatcataca gatgttgctc
     8341 ttcacgccca ccccagtaaa tggtgatatc gcgattggga ttacgcgcca acgccgtcaa
     8401 taaaatcgag cgcgcatagg agaacccggt gccgcccgca atcaaaatca tcggacgctc
     8461 ttcatcatcg cgcagccacg cttctccgtg gggaatatcg accacgattt gatgatcttt
     8521 gaggatgcgg tccatgactg ctttcgcgta aaggttgatt tcagaagcgc caatatgcag
     8581 ctcgataaac cctttttcat ccggcgtcga agccattgaa aacggacgtt tgtcgcgctc
     8641 atccatcact accatcaaat actgaccagc acgaaaagaa aaggccgcgt ctggtacgat
     8701 tcggacacgg tatacagtat ccgtgatagc ttctaccgag gtcactttac agcttaaggt
     8761 tgtcatgcgc tttctctgtc ggatcaaata aatagggcaa aactaacgcg catcaggcgc
     8821 ttttaccgtt gttaaaaata gccagttcat cccagatggc gtcaatatgc gcgacgacat
     8881 caggatcttt tttgatggga cgtccccatt cacgctgggt ttcccccggc catttattcg
     8941 tggcatccag ccccattttt gaacccagcc cggagacagg cgaggcaaaa tccagataat
     9001 caataggcgt attttctacc agaacagtat cccgcgccgg gtccatacgg gtggtaatcg
     9061 cccaaatcac atcgttccag tcgcgtgcgt tgacgtcatc atcgcaaacg atcacaaatt
     9121 tagtgtacat aaactggcgt aagaacgacc agacgcccat catgacgcgc ttcgcgtgtc
     9181 cggcgtactg ttttttgatt gtcactaccg ccagacgata agaacagcct tccggtggca
     9241 ggtaaaaatc gacaatttcc gggaactgtt tttgcagaat cggcacaaac acttcgttca
     9301 gtgcgacacc cagcaccgcg ggttcatccg gcggacgacc agtataggtg gagtggtaaa
     9361 tcgcatcttc acgctgggta atatgcgtca cggtaaatac cgggaaacta tcgacttcgt
     9421 tatagtaacc ggtgtggtcg ccatacggcc cttccggcgc agtttcgcct tgttcgatat
     9481 acccttccag cacaatctcc gcactggcgg gcacttcaag gtcattggag atacacttca
     9541 ccacttcggt cttggtaccg cgcagcaatc cggcaaacgc atactctgaa agtgtatccg
     9601 gaacgggggt gactgcaccg agaatcgtgg cgggatcggc acccagcgcc acagaaaccg
     9661 ggaaacgttc gcccggatgc gccgcacacc actcctgata atccagcgcg ccgccgcgat
     9721 gcgacagcca gcgcataatc agtttgtttt taccaatcag ctgctggcga taaatgccca
     9781 gattctgccg ctctttatgc gggccgcgcg ttacggtcag cccccaggta atcagcggcg
     9841 cggcatcttc cggccagcag gtcataatgg gaatgcgatt gagatcgacg tcatcgccag
     9901 agacgatttt ttgttggcag ggcgcaccac gcagccgctt tgtcggcatg ttcaatactt
     9961 gcttaaactg cggcagttta tcaaacaggt cgcggaaacc ttttggcggc tcgggctctt
    10021 tcagaaacgc caataattta ccaacttcac gcagcgccga aacatcttcc tgccccatgc
    10081 ccatcgccac gcgctttggc gtaccgaaca ggttgcacag caccggcatt gagtagcctt
    10141 tagggttttc gaacaacagc gcaggcccac cggcacgcaa agtgcggtca gcaatttcag
    10201 tgatttccag atgcggatcc accgggagcg tgatacgttt tagctcaccc tgctgttcaa
    10261 gcagcgtcaa gaagtcgcgt aaatcgttat atttcatggc gtccattgta gcctcttaat
    10321 ctacgcccat tatacggcgt tcatctttgc gatgctgtaa atttgttaaa ttagcgtgaa
    10381 ctctgacggt ataacgcaaa ccggggaata taattaactt agcgtaaagc ttttgctatc
    10441 cttgcgcccc gattaaacgg ataagagtca ttatgcaatc ttggtattta ctgtactaca
    10501 agcgcgggca acttcaacgt gcccaggaac accttgagag acaggctgtg aattgcctgg
    10561 caccgatgat caccctggaa aaaatcgtgc gtggaaaacg tactgcagtc agtgagccat
    10621 tgtttcccaa ctacctgttt gtcgaatttg atccagaagt gattcatacc acgactatca
    10681 acgcgacccg cggtgtcagc cacttcgtgc gctttggcgc gtcgctagcg atagtcccat
    10741 cggcggtgat tcatcagcta tcggtatata aaccgaaaga cattgtcgat ccggcaaccc
    10801 cttatccggg agataaggtg attattaccg aaggcgcgtt cgaaggcttt caggccattt
    10861 tcaccgaacc cgatggtgag gctcgctcca tgctattgct taatcttatt aataaagaga
    10921 ttaagcacag tgtgaagaac accgagttcc gcaaactcta aaacgcaatc ccaaaccgtg
    10981 ttttgacatt agcatccgtg gtggcagcca gccatgcggc atcttctcca cgccagtgcg
    11041 caatacgttg caaaatatgg ggcagatggg ctggctcgtt gcgccgggat gatggctttg
    11101 gcgtgagatc gcgagggagc agatacggcg catcagtttc aatcagtaat ttttccgccg
    11161 gaatcaacgg caaaagttcc cgcagctcca gtccgcgtcg ttcatcgtaa acccaaccgg
    11221 taatgccgat ataaattcca tgcgccacgc acgcctgcat ctcttcgcgt gtgccggtaa
    11281 agcaatgaag aaccgcacca ggcagtttat ccagccacgg ctccagcaat gtcataaacc
    11341 gctcgtgggc atcgcgacag tgcataaata ccggcatgtt taaatctgcg gcaatgcgta
    11401 gctgggcaac aaaagcgcgt tcctgctctt ccggcgtcga aaagttgcgg ttaaagtcga
    11461 gaccacattc accaatcgcc accacttctg gctgcgcggc cagctcaata atcgcttctt
    11521 cagtcgcagc ttgccactgg ctgctgtcgt gaggatgtac gcccgccgtt gaccaacagg
    11581 acgaatactg acgcgccagc ttttgcgcct gctggctttc acgcaggtta gtgccggtga
    11641 tgagtagccc attaactccc gcgtcaaaag cgcgcgctac aacatcatca cggtctttcg
    11701 caaattgcga actggtcaaa ttaacgccga tatcaaacat cctgtactcc atatgacaac
    11761 cgccctgacg ggcggttgaa tttattcttc agttttttcg ctttctgctt cagcgtcgtt
    11821 ttcctcttcc cgatttcgcc ctttaccaac gtaaaagcgt gagaagaaga caccgatttc
    11881 aaacagacag tacatcggga tcgccaatag cgtttgcgag aagacatccg gcggcgtcag
    11941 caacatcccg acaacgaatg caccaaccag cacatacggg cgttttttgc gtaagtcttc
    12001 tggcgaggta atccccatcc agcacagcag cacaattgcc accggcactt caaaggagac
    12061 accaaacgcc ataaacagcg ccataacgaa gcttaaatag ctggcgatgt cggtggatac
    12121 ctgcacccct tccggcgcgg tattggcaag gaagccaaat gccagcggaa agaccacaaa
    12181 gtaggcgaat gccatgccga tataaaacag cagagagctg gaaaccagca gcggcaccac
    12241 caggcgacgt tcatgcttat acagcgctgg ggcgataaat gcccacacct gatagagaat
    12301 caccggcgct gacagaatca gcgacaccat aaaggtcagc ttgatcggcg taaagaacgg
    12361 cgaggccacg tcggtggcga tcatcgttga accttgcggc aactgcttga tcaatggcgc
    12421 ggataccagg tgatagatgt cattggcgaa atagaccaga cacaggaata tcacgatcac
    12481 cgcgataatg cagttcagca gacgcttacg cagctcaatt agatgcgtga taagcggttg
    12541 agtatcttct acagacatgt ttacggttta tcactcgacg aaggggaagg tgcagcggtt
    12601 ttcggttcag cgtccgcagc aggttttacc accggctctg gcgtggtttc tggcttctgt
    12661 tccggcgaac tggcctgcgt ttgtgcagcg gcaggcgtta cgccctcatg cgcagcttca
    12721 ttatctttca ccaccgggtt atggatggtg tgcgcttcat cgctcgcctt ttcaggatcg
    12781 tttgcaacgt aggaacgctt catcgactcc gcggcctggc gtagttcatc catcgacgct
    12841 ttcagttcgg gcgtcaggtt agtgaggctc gccttttcaa cctttttcag actgtcctga
    12901 aactcctgga gttttaactc ctgggtcagt tcgttctgca ccgttgtcgc cagtgaacgc
    12961 aacgcgcgaa tccagcccgc taccgttttt accgccacag gcagtcgttg cggccccaga
    13021 acgacgaggc cgatgatgaa caccaatagc agttcgctaa aaccgatatc aaacacggat
    13081 tacacctgct ctttatcgtg gcgcttcgcg tcttctgttt tagcctgttc ctgattcgta
    13141 tccgcctgct tatcggcgat agttttcgca gtaaaatcag catcctgact ggttttatcc
    13201 tgctttggtt catcatcgct cattgctttt ttaaagcctt tgatcgacgc accaagatcg
    13261 gaaccgatgg agccgagctt tttggtgcca aaaagcagta caacgatgac ggcaataatc
    13321 aataactgcc aaatactgat accacccata catgttcctc tgtggtagat gatgattaaa
    13381 caaggccgca ttatacgtta cacgacccgc cttgagcgat gaaaaaatca gcgtgttttg
    13441 cgccaaccga caaaccaggc gatcagacca cctgccatta accagccggg catcagcccc
    13501 cattcaggtc ggctgaccaa caagaatgtg ccacttaata ctaacgtagc gccaattccg
    13561 agaaaataac gcgattgtcc ctgacgtaca tgatttgact gaagctcgcg ggcaatctta
    13621 tcaacactgt gctgtaaata cttgccctgg cgcaaactgt cgtaaaccaa ttcaggcagt
    13681 tctggcattt tttcgaccca gaacggcgct ttttctttaa atgctctcac cagggcagga
    13741 ataccgacct gatctttaat ccacgactcc aggaaaggct tcgctgtttt ccataaatcg
    13801 agctgcggat aaagctggcg tcctacccct tcgacataga gcagggtttt ctggagtaac
    13861 accagttgcg gctgcacttc catattgaag cgacgcgccg tattaaacag atttaacagt
    13921 acatgtccaa acgaaatttc ggccagcggt ttctcaaaga taggttcaca gaccgtacga
    13981 atggcaaatt cgaactcttc aacgttggta tctggtggca cccagccaga atcgacgtgt
    14041 agctctgcca ctttgcgata gtcgcgatta aagaaggcga taaagttttc tgccagatag
    14101 cgtttatctt ctttgtttag cgagccaaca atcccgcaat caatgccgat atatttcggg
    14161 ttttccgggt gttcatagct tacgaaaatg ttgccagggt gcatatcggc atggaaaaag
    14221 ctgtcgcgaa agacctgagt gaagaacacc tgcacgccac gttccgccag caatttcatg
    14281 ttggtgccat ttttctccag cgtcgcaaca tcagacaccg gaatgccgta aatgcgctcc
    14341 atcaccatca tcccttcact acaatagtca gggtaaactt ccgggatgta gagcatcggg
    14401 ctgtcttcaa aatttcgccg aagctgaatg gcgttggcag attcccgcag caaattcagt
    14461 tcatcaatca aggtcttttc gtactcgcgc accacttcgg ttgggcgcag acggcgacca
    14521 tccggcagca aacgcggcac ccagcgagcc agacggtaga taagtttcag atccgcttta
    14581 ataaccggca aaatatccgg gcggatgact ttaatcacca cctctttacc attcgatttc
    14641 aatcgcgcgg tatgaacctg ggcgatagaa gcagaagcca gtggcttgat ttcaaaatcg
    14701 tcaaaccacg cttctaccgg caagccgccc attgcagctt caatctgctg cttcgccagc
    14761 ttgccatcaa acggagcaac tttgtcctgc aataacgcca gctgatcggc aatatgcggt
    14821 ggaaaaagat cgcggcgggt tgataacatt tgcccgaact tgatccaaac cggccccagt
    14881 tcttgcaggg ccagtcgtag tcgctcacct aaaagtttgt ctttatgccg atttggcatc
    14941 cagaataatg agtatcgcca tagccgtagc ggcagggtga tacgcatttt ggggatcagt
    15001 tcatcaagtc cgtagcttaa aaaagtgcga atgatgaaat ataggcgccg tacttcacct
    15061 ggcgtcattt agcctccagt ttttccagcc gtttggtcag ggcatcaaca gcacgctcga
    15121 cggcagccgt ctcttccgca aaccaggcca cttcaagcgg accgggtgcc atacgccact
    15181 cttcagtaat ggcttccgcc acataacgtt gctggcgctt aatgccgtga tgcaggaact
    15241 ttgcgcctcc gcgcatggct ttgctgattc cttcagcggc gatatcaccg gtataagggg
    15301 ccagcagttc cgcagggtcg aactctgcca gatctgccag cgcaacgaag ttttgcacca
    15361 cctgaatatc gccctgcact tccagctcac cactgcgaat cagtgcggta agctgctggc
    15421 gatcgcgaag tttcggcaac acactggcgt aggcgataac ggtgcagtca gcatcgcctg
    15481 cccattcgcc cagtacatca acctggcgtt cgctgaacac cagaatcaat gacgtcgaaa
    15541 agccttttac ctccacgcgc aatactttac ccagcagacg cgagcgggct gttttcagcg
    15601 cgggtgagcg atacaggaag gtgttgagca gactttcaat tcctgccgtc actaaaggtt
    15661 taaaaggcat ttccggtctc ctgtcagaac ttataaccac gatgcagcgc cacaacccct
    15721 gccgtcagat tgtagtagtc gacactttcg aatccggcat cctgcatcat ggctttcagg
    15781 gtatcctgat cgggatgcat acggatggat tctgccagat aacggtagct gtcggcgtcg
    15841 ttcgcgacca gtgagccaat acgcggcagc acatggaagg agtatgcatc ataggctttg
    15901 ctcagcggct cgataattgg cttcgagaac tcaagcacca gcaggcggcc gccgggtttc
    15961 agcacgcgat acattgaacg cagtgcttta tctttgtcgg tgacgttacg cagaccaaac
    16021 gaaatggtga tgcaatcaaa ggtgttatcc gggaacggca gcgcctcagc gttcgcctga
    16081 acatactcaa cgttgccaat cacaccgata ttacgcagct tctcgcgtcc cattttgagc
    16141 atggattcat tgatatcagc aaggaccact ttgccagttt ctccgaccag gcgggagaat
    16201 ttcgctgtca ggtcgccggt gccaccagcc agatccagca cggtctgccc acggcgtacg
    16261 ccgctgcaat caatcgtgaa tcgcttccac aaacgatgaa taccaaatga catcaaatca
    16321 ttcatgacat cgtattttga tgccacggaa tggaaaacgt gggcgaccat atccgctttt
    16381 tgttccttcg cgacggtctg aaaaccaaag tgcgtcgttt cttgtgactt atccaccatc
    16441 tcaatgcctg ctcatcaaaa aattgttcca gaagtgtaac agattgggcg tcgatgccct
    16501 agatttctac ccggcttaac tacccctaat gggctagcgc gactgctgat tatattcatc
    16561 atcgcgttga taagcttcat cattcggctg ctccggaacc gaccgaagtc gatactcttc
    16621 attctggctc accgcctgtt cagccaaatc cggattaatc tcgcgtttaa tttctactcc
    16681 taaaccgcga aacgcttctg cctgcgccag cacatttccg cgacctgaag agagtttttt
    16741 cattgcctgg cggtagttat cctgcgcttt gtcgagactt tgaccaatcg cggacatgtc
    16801 atcgatgaac aaacgcatct tgtcgtacag cttgctggca cgatcggcga tttgctgggc
    16861 gttgcggctt tgatgctcat aacgccacag gttggcaata gtacgcaacg ccaccagcag
    16921 cgtagtcggg ctaaccagca tgatattgtt tttcaacgct tcggtgatca gctccggctg
    16981 gcggtcaagc gccagtaaaa aagcgggttc aacgggaata aacatcagca cgtaatccag
    17041 agttcgcagc cccggcagct gttgataatc tttgcgtccc agcaaacgga tatggttacg
    17101 caccgacgcg atatgttcct gtagcgcgct ttcgcgggtg tagtcgtctt cggcgttaaa
    17161 atagcgttca taggcgacca gcgtcatttt ggcgtcgatc accacatctt ttccctgcgg
    17221 cagccgcacg atgacatccg gctgcatccg cgagcgggcg tcattttcga tgctgacctg
    17281 ggtttcatat tcataccctt cacgcagacc ggaagcctcc agcacccgcg tcaatactac
    17341 ctcgccccag ttgccctggg ttttattgtc gcctttcagc gcgcgcgtca ggttgatcgc
    17401 ttcctgggcc atttgcgcgt tgagttgctg gagattgcga atttcgtggg tcagggtatg
    17461 gcgttcttgt gcttctttac cgaagctgtc ctgaacctga cggcggaaac cgtccagttg
    17521 ttcacgtagc ggcgacaaca ggctgttcag actctgacgg ttttgctcat caacccggcg
    17581 attgctgtgc tcaaaaatac ggttggcgag gttttcaaac tgctcactga ggcgctgctc
    17641 gctgttaatc atctggcgaa ttttatcgtc agcatgttgc tgtgcggctt ccatccgcgt
    17701 ggttacttca cgcagatcgg cctccagaga ggtgttaata ctttgcaggc tgcgcacttc
    17761 gttattgagt aactcgcact ctgcacgcca gtgctcgctt tgggtaattt gttgttttgc
    17821 cgcgcttaac tccgcgacca tctcttcacg ttcagctaat tgctcggctt tttgctgcgc
    17881 atgttgataa ctggcaaaca gccagccaat tgccacaccc accaacgcaa taactgcgta
    17941 aaccatgatt gagaaatcca caacgcctcc tgacgcaaca acttacccgc acaaaataga
    18001 attgtgctgt acaaacgtcc agttggaaag gcatttcctg cgaggcgcta cgcaaaaata
    18061 caaaaggccg aacgcgtcgg ccttcagaca ggagaagaga attacagcag acgacgcgcc
    18121 gcttccacca cgattttcac cgcttggctt tcagtttgtt tcatcgtctc agcattcggg
    18181 atctcttgct gggtgcggtt aacgataaca cccgctacca taccggcacg caggccctga
    18241 cttgcacaca tggtcagcag ggttgcagat tccatttcat agttcattat gcccatcgcc
    18301 tgccactctt ccatagaacc tttaaagtga cgaactacgc gaccagagta agtatcgtaa
    18361 cgttcctgac ctgggtagaa ggtatcagaa gaagctgtca cgccaacgtg agttgtcgcg
    18421 ccaatggatt tcgcagcttc aaccagcgca gtcgtacatt cgaaatcagc gacagccggg
    18481 aattccagcg gtgcgaagtg caggctcgcg ccatccagac ggacagacgc cgtggtaacc
    18541 aggacatcac ccacattaat atgcggctga atagcgcccg ttgtaccgat acgcaggaag
    18601 gtgcgaatgc ccagctgtgc cagctcttca acagcaatag aggtagacgg gccgccgata
    18661 ccggtagagc agacgataac aggtttacca tccagctctg cacgccaggt agtgaattcg
    18721 cggtgagatg ccagcttaac cggcttatcc atcagcgcgg cgatcttttc cacacgatcc
    18781 gggtcgccag ggacgatggc aagcgtagcc ccttgtaaat cgtttttagt gaggccgaga
    18841 tgaaaaacat cagacttgga catatacaac tcctctgtga atcggtttag tcagaagaag
    18901 caaaaagaca ctttaccgaa gggtttaaca ttttttcgtg atactcatca ccatgacgca
    18961 aatgcgttgc ataaatttac ccaccaaaag tgattcaaat cacataaatc actcttcatt
    19021 ctgcaggact gcataaaaga tgagccgatt caggcaatgt gatttgttgc atgctgacta
    19081 tagttaacat tgcgcaacat ttttataagg gtacggagaa taccatggca acaacacaac
    19141 aatctggatt tgcacctgct gcatcgcctc tcgcttcgac catcgttcag accctggacg
    19201 acgcgattgt ggcgggcttc acctctatcc cttcacaagg ggataacatg cctgcttacc
    19261 atgccagacc aaagcaaagc gatggcccac tgccagtggt cattgtagtg caggaaattt
    19321 ttggcgtgca tgaacatatc cgcgacattt gtcgccgtct ggcgctggag gggtatctgg
    19381 ctatcgcacc tgaactttac ttccgcgaag gcgatccgaa tgattttgcc gatatcccca
    19441 cgttgcttag cggtctggta gcaaaagtgc ctgactcgca ggtgctggcc gatctcgatc
    19501 atgccgccag ttgggcttcc cgcaacggcg gcgatgttca tcgtttaatg atcaccggat
    19561 tctgctgggg tggacgtatc acctggctgt atgccgcgca taatccacag ctaaaagctg
    19621 cagtggcgtg gtacggcaaa ctgacgggcg acaagtcgct gaattcaccg aaacaacctg
    19681 ttgatatcgc aaccgatctt aacgcgccga ttctcggctt atatggcggt caggataaca
    19741 gcattccgca ggagagcgtt gaaaccatgc gccaggcgct gcgggctgct aatgcgaaag
    19801 cagagattat cgtgtacccg gatgccgggc atgcattcaa cgctgattat cgcccgagct
    19861 atcatgccga atctgcgaaa gatggctggc agcgtatgct ggaatggttt aagcagtatg
    19921 gagagaagcg gagttagttt cctcttagtc tatatttgag atggtgagta aacgagtgcg
    19981 ttttgctgcc atctctgtcc cccaaaaaat aatcagtatg aattgattgg agattacgac
    20041 tccgctattt gaccttaaga aagtcggcaa gtcatagtca taaatgagtc tcttcaatgg
    20101 aagacacctt agagatttac catcaactcc cattcacaaa caaacattaa ttattatcaa
    20161 cacattcaca ttaattataa atcatctcag accttcctga taaaaagata ttaaagtgaa
    20221 acattttgtt tttcacattt tagttcatca cttttaggcg taaataaagg taaaaaaaac
    20281 aacaatacta tttctctttc acaaaagaat taacaaaagg tttttcaagg atgagtgata
    20341 ttatttacct gaccataacc ggagaacaac aaggatgtat ttcctctcgt tgtggaacga
    20401 gcgcatcaat aggtaatcgc tggcagattg gccatgatga tgaaatcttc gcgttctcgc
    20461 tctctaatag tattacgaat acaggcaaag ggtcataatt gcatggcctt agtttctgta
    20521 aattgattga caaaagctcc ccgttactta ttaacgctat taacaataac gagcagttgt
    20581 tcatggagtt tgacttctat cggatcaata ggtttggtcg atgggagaaa tattacaaca
    20641 ttcaactccg cggcgcgctc ttatcagcaa tcaatcacct gtttactgag aacaatctgg
    20701 atacagaagc catcactgtc agttattaat atatactctc caggcatctg attgctaata
    20761 ctgaatttag ttattgggca tttcccgata actacaaccg tctgtttata ccacggccaa
    20821 aaactaaagc tggtaatggt ttcaaaacac ttaatagtaa agcggttggc agactgcttg
    20881 ctgctgtcgg catatacaat gggaatatcg aaggattcag ggaaacagca gacaagttgg
    20941 ggggagatgc acctgctggt tatgatcaat ttatggataa caaagggtta ctcatcgctg
    21001 gtgcttcaat agttgctgga gcagttctgg ggagaatgag actccctgat ttggaatcac
    21061 ttgagcattt cggagccaga ggagcagtat caggaagacc atttaatccc gagcttgccg
    21121 gtggtccaat agaaaacctg acgacagatg gcgtaacaat caacagggaa ggtatcgcta
    21181 ttgttgaaaa acatatagcc agatttggtc atgatcctgt aaatgaagtg atgatcaata
    21241 gactgaaaga cattgagaaa ggaaaaatcc ccccagaaca agtagatctt aacttctata
    21301 ctcatgagtg tcgggaatat cagcgttact gtaatcttgg ttgggaaaca ggacaacctg
    21361 atggtgacgc aggctatgca ctctggaatc atactggatt tgcccctata tttccagaca
    21421 tctgttatca cttaacccat tacaagcccg ctgcc
//
LOCUS       DAEEKQ010000059        21005 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.059, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000059 DAEEKQ010000000
VERSION     DAEEKQ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.059"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..172)
                     /locus_tag="KLK57_002115"
     CDS             complement(<1..172)
                     /locus_tag="KLK57_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892479.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYG"
     gene            344..1564
                     /gene="coaBC"
                     /locus_tag="KLK57_002116"
     CDS             344..1564
                     /gene="coaBC"
                     /locus_tag="KLK57_002116"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HBE0892480.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEVAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            1542..2000
                     /gene="dut"
                     /locus_tag="KLK57_002117"
     CDS             1542..2000
                     /gene="dut"
                     /locus_tag="KLK57_002117"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HBE0892481.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDSTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            2107..2703
                     /gene="slmA"
                     /locus_tag="KLK57_002118"
     CDS             2107..2703
                     /gene="slmA"
                     /locus_tag="KLK57_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HBE0892482.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="KLK57_002119"
     CDS             complement(2740..3381)
                     /gene="pyrE"
                     /locus_tag="KLK57_002119"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HBE0892483.1"
                     /translation="MKPYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KNHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(3447..4163)
                     /gene="rph"
                     /locus_tag="KLK57_002120"
     CDS             complement(3447..4163)
                     /gene="rph"
                     /locus_tag="KLK57_002120"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HBE0892484.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            4290..5153
                     /locus_tag="KLK57_002121"
     CDS             4290..5153
                     /locus_tag="KLK57_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HBE0892485.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            5375..6199
                     /gene="dinD"
                     /locus_tag="KLK57_002122"
     CDS             5375..6199
                     /gene="dinD"
                     /locus_tag="KLK57_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HBE0892486.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFRQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAI
                     DFAIFQNHGYRGLYGGLDQKAIHQRKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSINQLENSVKITEKK"
     gene            6491..7108
                     /locus_tag="KLK57_002123"
     CDS             6491..7108
                     /locus_tag="KLK57_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HBE0892487.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="KLK57_002124"
     CDS             complement(7105..8787)
                     /gene="ligB"
                     /locus_tag="KLK57_002124"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HBE0892488.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEIARVRNEWWKAKLPFVT
                     DGVVVRAAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQRLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            9045..9668
                     /gene="gmk"
                     /locus_tag="KLK57_002125"
     CDS             9045..9668
                     /gene="gmk"
                     /locus_tag="KLK57_002125"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HBE0892489.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            9723..9998
                     /gene="rpoZ"
                     /locus_tag="KLK57_002126"
     CDS             9723..9998
                     /gene="rpoZ"
                     /locus_tag="KLK57_002126"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HBE0892490.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            10017..12125
                     /gene="spoT"
                     /locus_tag="KLK57_002127"
     CDS             10017..12125
                     /gene="spoT"
                     /locus_tag="KLK57_002127"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HBE0892491.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            12132..12821
                     /gene="trmH"
                     /locus_tag="KLK57_002128"
     CDS             12132..12821
                     /gene="trmH"
                     /locus_tag="KLK57_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HBE0892492.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            12827..14908
                     /gene="recG"
                     /locus_tag="KLK57_002129"
     CDS             12827..14908
                     /gene="recG"
                     /locus_tag="KLK57_002129"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HBE0892493.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACMTEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="KLK57_002130"
     CDS             complement(15077..16282)
                     /gene="gltS"
                     /locus_tag="KLK57_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HBE0892494.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            16562..17953
                     /gene="xanP"
                     /locus_tag="KLK57_002131"
     CDS             16562..17953
                     /gene="xanP"
                     /locus_tag="KLK57_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HBE0892495.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            18074..19783
                     /locus_tag="KLK57_002132"
     CDS             18074..19783
                     /locus_tag="KLK57_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HBE0892496.1"
                     /translation="MKFIGKLLLYILIALLVAIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMVHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSAE
                     LVQ"
     gene            complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="KLK57_002133"
     CDS             complement(19835..>21005)
                     /gene="yicI"
                     /locus_tag="KLK57_002133"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBE0892497.1"
                     /translation="LAIYDFTNPDACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQW
                     FDGSDPQKMHNHYAYIYNELVWNVLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYA
                     NYESMAESLRGGLSIGLSGFGFWSHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSK
                     SYRVPWAYDDESCDVVRFFTQLKCRMMPYLYREAARAKARGTPMMRAMMMEFPDDPAC
                     DYLDRQYMLGDNVMVAPVFTEAGEVQFYLPEGRWTHLWHNNELDGSRWHKQQHGFLSL
                     PVYVRDNTLLALGNNDQRPDYAWHEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAT
                     RTGNTITVTGAGEARNWTLCLRNIVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
BASE COUNT         5083 a         5713 c         5387 g         4822 t
ORIGIN      
        1 agccataaag agagccgaaa ttctccagca tctcttttgc cagggttaat acatctttac
       61 cgcgcgttcc ggtacgcaga aatagcgcca gcagctcgac atccgttaag gcgctaatac
      121 caaacttcag cattttttcg cgcggcatca acagctgtgc attgtttttc accttcacct
      181 cctttgtggt gctcgcatcc tgtcatagcc gtttcgttaa atcgacggcc agttttcaat
      241 cctggaaagc gcctcgcaaa gtgaatctca ggtgaaggca cgacaacaaa agtattgtga
      301 taaaatcgcc aacttctggt gtcaccctac aggaaaaatc atcatgagcc tggccggtaa
      361 aaaaatcgtt ctcggcgtta gcggcggtat tgctgcctat aaaacccctg aactggtgcg
      421 tcgtttgcgc gatcgcgggg ccgacgtccg cgtagccatg accgaagcgg caaaagcctt
      481 tatcacccca cttagcttgc aggcggtttc tggttatccc gtttccgaca gtctgctgga
      541 cccggcagcc gaagccgcta tgggccatat tgagctgggt aaatgggctg atttagtgat
      601 tctcgcccct gccacggcag atttgattgc ccgtgttgct gccggaatgg cgaatgacct
      661 ggtatcgacg atttgtctgg ctacacctgc gcctgtagcc gtgctccccg ccatgaacca
      721 gcagatgtac cgtgccgctg ccacgcagca taatttagag gtgcttgctt cccgtggttt
      781 gctcatctgg gggccagaca gtggcagtca ggcttgtggt gatatcggtc ctgggcgaat
      841 gctcgatccg ttaaccattg tggatatggc ggtagcgcat ttttcgcccg tcaacgacct
      901 gaaacatctg aacattatga ttaccgccgg cccgacgcgt gaaccgctcg atccggtgcg
      961 ttatatctct aatcacagct ccggcaagat gggttttgct atcgccgccg ccgctgcccg
     1021 tcgtggcgcg aacgtcacgc tggtatcagg cccggtttcg ctaccgacgc caccgtttgt
     1081 taaacgtgtt gatgtgatga ccgcgctgga aatggaagtc gccgtgaatg cttctgtaca
     1141 gcagcaaaat atttttatcg gctgcgccgc cgtggcggat tatcgcgcag ctaccgtggc
     1201 cccagagaaa atcaaaaagc aggccacgca gggtgatgaa ttaacaataa aaatggttaa
     1261 aaaccccgat atcgtcgcag gcgttgccgc actaaaagac catcgaccct acgtcgttgg
     1321 atttgccgcc gaaacaaata atgtggaaga atacgcccgg caaaaacgta tccgtaaaaa
     1381 ccttgatctg atctgcgcga acgatgtttc ccagccaact caaggattta acagcgacaa
     1441 caacgcatta caccttttct ggcaggacgg agataaagtc ttaccgcttg agcgcaaaga
     1501 gctccttggc caattattac tcgacgagat cgtgacccgt tatgatgaaa aaaatcgacg
     1561 ttaagattct ggacccgcgc gttgggaagg aatttccgct cccgacttat gccacctctg
     1621 gctctgccgg acttgacctg cgtgcctgtc tcgacgacgc cgtagaactg gctccgggtg
     1681 actctacgct ggttccgacc gggctggcga ttcatattgc cgatccttca ctggcggcaa
     1741 tgatgctgcc gcgctccgga ttgggacata agcacggtat cgtgcttggt aacctggtag
     1801 gattgatcga ttctgactat cagggccagt tgatgatttc cgtgtggaac cgtggtcagg
     1861 acagcttcac cattcaacct ggcgaacgca tcgcccagat gatttttgtt ccggtagtac
     1921 aggctgaatt taatctggtg gaagatttcg acgccaccga ccgcggtgaa ggcggctttg
     1981 gtcactctgg tcgtcagtaa cacatacgca tccgaataac gtcataacat agccgcaaac
     2041 atttcgtttg cggtcatagc gtgggtgccg cctggcaagt gcttattttc aggggtattt
     2101 tgtaacatgg cagaaaaaca aactgcgaaa aggaaccgtc gcgaggaaat acttcagtct
     2161 ctggcgctga tgctggaatc cagcgatgga agccaacgta tcacgacggc aaaactggcc
     2221 gcctctgtcg gcgtttccga agcggcactg tatcgccact tccccagtaa gacccgcatg
     2281 ttcgatagtc tgattgagtt tatcgaagat agcctgatta ctcgcatcaa cctgattctg
     2341 aaagatgaga aagacaccac agcgcgcctg cgtctgattg tgttgctgct tctcggtttt
     2401 ggtgagcgta atcctggcct gacccgcatc ctcactggtc atgcgctaat gtttgaacag
     2461 gatcgcctgc aagggcgcat caaccagctg ttcgagcgta ttgaagcgca gctgcgccag
     2521 gtattgcgtg aaaagagaat gcgtgagggt gaaggttaca ccaccgatga aaccctgctg
     2581 gcaagccaga tcctggcctt ctgtgaaggt atgctgtcac gttttgtccg cagcgaattt
     2641 aaataccgcc cgacggatga ttttgacgcc cgctggccgc taattgccgc gcagttgcag
     2701 taatatgacg ccggatgact tttcatccgg cgagtttctt taaacgccaa actcttcgcg
     2761 ataggcctta accgccgcca gatgttccgc catttccggc ttctcttcca ggtaagcaat
     2821 caggtctttc agggtgatga tagagatcac tttgcagttg taatcacgct caacttcctg
     2881 aatcgccgaa atctcgctgc gcccccgttc ctgacgatcg agcgaaatca acacgccagc
     2941 aagcgtcgcg ccattggcct gaataatttc catcgactca cgaatcgccg ttccggcggt
     3001 gatcacatca tctaccagca ttacgcgtcc ttgtaacgcg ctaccaacca gattgccgcc
     3061 ttcaccgtgg ttttttgcct ctttgcggtt aaagcagtac ggcaggtcca ggtcatgatg
     3121 ctccgccagc gccacggcgg ttgtggtggc aatcgggatc cctttgtaag cagggccaaa
     3181 cagcagatcg aactcaatgc cggaatccac caatgcttca gcgtaaaaac ggcctaacag
     3241 tgccaggtcg cgcccggtat taaacagccc ggcgttgaag aaataggggc ttttacgccc
     3301 ggatttcagc gtaaactcgc caaactttaa cacctgcttg ctaagcgcaa attcaataaa
     3361 ctggcgctga tatggtttca tgccttcgct cctcatctta cttttctaca gacaaaaaaa
     3421 aggcgactca tcagtcgcct taaaaatcag tttgccagcg ccgccttctg cgtcgctaca
     3481 atggattcga ttccccctcg ggccagagcc aacaaggtga gtaattcttc gtgggtgaac
     3541 ggttcgcctt ctgccgtccc ctgcacttca atgatgcgcc cgtcttcggt catcactacg
     3601 ttcatgtcgg tctctgcggc agagtcttca acgtattcca gatcgcaaac cgcttcgccg
     3661 ttcacaatcc cgacagaaac cgcggctacc atccctttca tcggattggt tttcagctta
     3721 ccgttttcca ccagcttctg tagcgcatct gccagcgcca cgcaggcacc cgtaatcgaa
     3781 gcggtacgag tgccaccatc agcctgaagc acgtcgcagt ccagcgtaat ggtgaactca
     3841 cccagcgctt tcaaatctac tgccgcgcga agcgcacggg cgatcagacg ctggatttcc
     3901 attgtgcgtc caccctgctt acctttcgcc gcttcacgag cgttacgggt gtgggtagaa
     3961 cgcggcagca taccgtactc tgcggtgatc cagccctggc cctgaccttt caggaagcgc
     4021 ggcacgcctt cttcaataga ggcggtacac aacactttgg tatcgccaaa ttcgaccagc
     4081 accgagcctt ctgcatgttt tgtatagtta cgagtcaggg taacgggacg cacctgatta
     4141 ttgctacggc ctgctggacg catattgaaa tctccggctt gaaacaaatg tggctgcgca
     4201 ttatacggac ttccggaggt tattcctatc ctgacaaggc atcgatggct ataatccttc
     4261 cacctctcct tttataaaca ggaacgtcta tgatccgcag tatgaccgcc tacgcccggc
     4321 gtgaaatcaa gggtgaatgg gggagcgcaa cctgggaaat gcgctcggta aaccagcgtt
     4381 atctggaaac ttactttcgt ctgccggagc agttccgtag ccttgaacct gtcgttcgcg
     4441 agcgtattcg ttctcgcctg acgcgcggta aagtggaatg taccctgcgc tatgagccag
     4501 atgttagcgc gcaaggtgag ctgatcctca acgaaaaact ggctaaacag ctggtaactg
     4561 ccgcgaactg ggtaaaaatg cagagtgacg aaggggaaat caacccggtt gatattctgc
     4621 gctggccggg cgtaatggca gcccaggagc aggatctcga cgcgattgcc gctgaaattc
     4681 tcgcggcgtt ggatggtacc ctggacgact ttatcgtcgc acgtgaaacc gaaggtcagg
     4741 cgctgaaagc gttgatcgag cagcgtctgg aaggcgtcac cgccgaagtg gtcaaagtcc
     4801 gcgcccatat gccggaaatc ctgcaatggc agcgtgagcg tctggtcgcg aagctggaag
     4861 atgctcaagt gcaactggaa aacaaccgcc tggagcagga gttggttctg ctggcacaac
     4921 gaattgatgt tgccgaagaa ctggaccgcc tggaagcgca tgtcaaagag acctacaaca
     4981 ttctgaagaa aaaagaagcc gttggtcgtc gtctggactt tatgatgcaa gagttcaacc
     5041 gcgagtcgaa cactcttgcg tcgaaatcta tcaatgccga agtgacgaat tctgccatcg
     5101 agctgaaagt gttgattgag cagatgcgcg agcagattca gaatattgag taactcgtaa
     5161 cactaattct tacctttcag ccttcacaaa tgccgcccac tcaaacagag cggcattttt
     5221 cttccccgca acattcaatt ctgttttgcg tacctgctcc agattttgcg atgttttttt
     5281 gcgcagcgca gtgagaacgt gagatactca caactgtata taaatacagt tatagactta
     5341 ctttctttgc aatcgatatc acatggagtg ggcaatgaac gaacatcatc aaccttttga
     5401 agaaatacgg cattatggca ctgaagggca agaattttgg tcagcgagag aattggcacc
     5461 gttactggat tatcgggact ggcgcaattt tcaaaaggta ctggcccgcg cgactcaagc
     5521 gtgcgaagcc agcaatcaag ctgcatctga ccatttcgtt gaaaccacca aaatggttgt
     5581 attaggttca ggggctcaac gagaacttga agatgttcat ctctctcgct atgcctgtta
     5641 cctggtagta caaaacggcg accctgcgaa accggttatt gcggcagggc aaacttattt
     5701 tgctatccag acccgacggc aggagctggc tgacgatgaa gctttcaggc aacttcgtga
     5761 agacgaaaag cgtctttttc tgagaaatga gttgaaagaa cataacaaac aattagttga
     5821 ggcagcgcag caagctgggg tagcgacagc tatcgatttc gccatatttc agaatcatgg
     5881 ttaccggggg ctgtatggtg gattagatca gaaagccatc catcagcgga aggggctgaa
     5941 aaagagtcag aagatcctgg atcatatggg ttcaacagaa ctggcggcta atctctttcg
     6001 agctacccaa acagaagaaa aactcaagcg ggatggcgta aattcaaaac agcaagctaa
     6061 taccacacac tttgacgtgg gtagcaaggt gaggcaaacc attcaggaac ttggcggaac
     6121 catgcctgag gagttgccga ccccgcaggt cagcatcaat cagttggaaa acagtgtaaa
     6181 aattacagag aagaaatagc agaaactaaa aactcttttt cttaattgag atacccgatg
     6241 cgtaagccaa agtccaggtg cagtgaacac aatggctaaa cattgcacct ttcttttcca
     6301 cccctcaatt ttaacctatt ttttcttatg cattttctca gacaagaagt cagaagaatg
     6361 catctacact acagaaaata gcgatttcac ataactacaa gttatcaatt tcctctcacc
     6421 ttaaaaaatc tcaatcgtga caatacgcac aaatcgctac cctgccagac agatttttag
     6481 ggagagaacc atgctgttac acattttgta tttggttggc attactgccg aagccatgac
     6541 gggggcgctg gcggccggac gacggcgcat ggatacattt ggcgtaatta ttattgcgac
     6601 cgccaccgca attggcggag ggtcagtgcg cgatattctg ctgggccact atccgctcgg
     6661 ttgggtcaaa cacccggaat atgtgattat cgtcgctacc gccgcagtac tcaccactat
     6721 cgttgctcct gtaatgcctt acctgcgcaa agtgtttctg gtgctcgatg cgctcggact
     6781 ggtggtcttt tctatcatcg gcgcacaggt tgcactggat atggggcacg gcccaattat
     6841 tgccgttgtc gcggcggtaa ccaccggcgt gtttggcggc gttttgcgcg atatgttctg
     6901 taaacgcatc ccactggtat tccagaaaga gttgtatgcc ggtgtctcat ttgcctccgc
     6961 cgtgctgtac atcgcgctgc aacactatgt ttctaaccat gatgtggtga ttatctccac
     7021 cctggtattc ggcttttttg cccgcttact ggcgctacgt cttaagctgg gattaccggt
     7081 cttttactac agccacgaag gtcactaagg ttcaaaacct gtgatctgct gggcagccag
     7141 ccaactgccc agcttcttga tttgcgcatt ttccttccat tcaataacct gtctggcgcg
     7201 cccagatcca gtccccggca gccgctgcca gaactgctcc gtgctaaata aaagttgcga
     7261 ccaggaccgt tcatcactgg cattaagcgc cgcccgggtt aacggtattc ccattgccat
     7321 cacccagcga gtaaaaggct gcttacgagc cagattaaac tgatgccata actgcgcact
     7381 tttacttttc gcgatccccg gcgtgttctg taattgctct ggcgttaata aaagccagga
     7441 aaagatatgc tcaaagcgat gagtctgatg cagcgcgcgc cagccggcct caccaatgcc
     7501 atccagccca agaacctgtt ttgaccccag ccagactaag cgtgaaatga actgttcctg
     7561 acaaacatca gaagcaaagt agcaggtcaa cgagttaaag cggttttctg gtggtgtcgg
     7621 ttttgtacgt tctgcaccgc gccagaccac atcatcaatg cgaggaatac cctgaccggc
     7681 aaggctgacg aggatctgat caccaggcgc aatatcccac tcctgccagc gcctgacgga
     7741 accaatattc acccgctgga cttttttatc atccagcatg acaggtgcga gtgacgcaac
     7801 caccgatatt ttaccgctct tacccaccgc aaactgaatc gccttcactt cggcaacctg
     7861 agctacaggt tgatatttcc aggccaccaa ccattctgcc tggcctggta gccaatggcg
     7921 ggattcgggt tccttagccg ctcgcacaac cacgccatcg gtgacgaagg gtaatttcgc
     7981 tttccaccac tcattgcgta cgcgcgcaat ttcatcagca tttttaaccg cacgggtata
     8041 cctctgcgtt agagtaaaac ctgcggtagc cagctccttt aaacgatcgg tcattaactg
     8101 cggtccatcc ggccatgccc agacaaaaac agccagtgag tttagcgtgt cgctattccc
     8161 ctggcgcatc atcaggccag caacttttgc gcgggcattt attcccccca tttgttgttg
     8221 gatatgcccc tcgcgctgga gaaatatttc cccctgaagc gtactgttgg ctaaaggccc
     8281 gctaacggtt tgcggcacag cggaaattaa gctaactttc tgcgtccagt cctcgccttt
     8341 caggccatta ccgcgactga ttgctttgtt cagtttcccg tcccgataaa ccagggttac
     8401 cgcaacgcca tcaacttttg gctgcaccca aagatcgcta cgttctcgca tccacagact
     8461 taatgcgttt ttatccacca ttttgcgtac acccgtatgg gcaaccggat gcataaccgc
     8521 accatttaaa ggcggcatca tcacgtcgcg gggctcgctc ccaaagcagc gttgccactg
     8581 cgtaagacgg gcgcttaact gatcgtaaac accgtcttcc acctcacttt tcccttcctt
     8641 ccagtagtca tcgtcccact gttttatttg ctgttgcagg cgggaaattt cttcctgtgc
     8701 tctggctggc gaccaggccg gacagaccgc ccacacagat gattgccagc acaagatact
     8761 tattaatatc gccatccata ctttcatcat cacctccgct gtagatagtc aggcagatat
     8821 acaacgtgat gaaagcaaag ccgagtggca aaaacggagt ctgcgaggac gcttcctgaa
     8881 aatcgtcttt attgcaatga atgacaggca aatgcggaag cagctacgca aaacgcaaca
     8941 actttgcgca aaaagtgtga gcaagggcta cgtcacatgg ccgcgccgtg tataataagc
     9001 tcgtatgtag gctttatttc gctaatcaca tacgaaagat actcatggct caaggcacgc
     9061 tttatattgt ttctgccccc agtggtgcgg gtaaatccag cctgattcag gctttattaa
     9121 aaacccaacc gttgtatgac acccaggttt ctgtttcaca caccacacgc caaccgcgtc
     9181 ctggtgaagt ccacggtgaa cattatttct ttgttaatca tgatgaattt aaagaaatga
     9241 ttagcagaga tgcgttcctc gaacacgcag aagtttttgg taattactat ggcacttcgc
     9301 gtgaggccat tgagcaagta ctggcgaccg gtgtcgatgt ttttctcgat atcgactggc
     9361 agggcgcgca gcaaattcgc cagaagatgc cgcacgcgcg gagtatcttt attttaccgc
     9421 cgtccaaaat tgaactggac cgccgtctac gcggtcgcgg tcaggacagc gaagaggtca
     9481 ttgcaaagcg tatggcgcaa gctgttgcag aaatgagcca ttacgccgaa tatgattatc
     9541 tgattgtgaa tgatgacttc gataccgcgt tgaccgattt gaagaccatt attcgcgccg
     9601 aacgtctgcg catgagccgc caaaagcagc gtcatgacgc tttaatcagc aaattgttgg
     9661 cagactgaac ctgatttcag tatcatgccc agtcatttct tcacctgtgg agctttttaa
     9721 gtatggcacg cgtaactgtt caggacgctg tagagaaaat tggtaaccgt tttgacctgg
     9781 tactggtcgc cgcgcgtcgc gctcgtcaga tgcaggtagg cggaaaggat ccgctggtac
     9841 cggaagaaaa cgataaaacc actgtaatcg cgctgcgcga aatcgaagaa ggtctgatca
     9901 acaaccagat cctcgacgtt cgcgaacgcc aggaacagca agagcaggaa gccgctgaat
     9961 tacaagccgt taccgctatt gctgaaggtc gtcgttaatc acaaagcggg tcgcccttgt
    10021 atctgtttga aagcctgaat caactgattc aaacctacct gccggaagac caaatcaagc
    10081 gtctgcggca ggcgtatctc gttgcacgtg atgctcacga ggggcaaaca cgttcaagcg
    10141 gtgaacccta catcacgcac ccggtagcgg ttgcctgcat tctggccgag atgaaactcg
    10201 actatgaaac gctgatggcg gcgctgctgc atgacgtgat tgaagataca cccgccacct
    10261 accaggatat ggaacagctt tttggtaaaa gcgtcgccga gctggtagag ggggtatcga
    10321 aacttgataa actcaagttc cgcgataaga aagaggcgca ggccgaaaac tttcgcaaga
    10381 tgattatggc gatggtgcag gatatccgcg tcatcctcat caaacttgcc gaccgtaccc
    10441 acaacatgcg cacgctgggc tcacttcgcc cggacaaacg tcgccgcatc gcccgtgaaa
    10501 ctctcgaaat ttacagcccg ctggcgcacc gtttaggtat ccaccacatt aaaaccgaac
    10561 tcgaagagct gggttttgag gcgctgtatc ccaaccgtta tcgcgtaatc aaagaagtgg
    10621 tgaaagccgc gcgcggcaac cgtaaagaga tgatccagaa gattctttct gaaatcgaag
    10681 ggcgtttgca ggaagcggga ataccgtgcc gcgtcagtgg tcgcgaaaag catctttatt
    10741 cgatttactg caaaatggtg ctcaaagagc agcgttttca ctcaatcatg gacatctacg
    10801 ctttccgcgt gatcgtcaat gattccgaca cctgttatcg cgtgctcggg caaatgcata
    10861 gcctgtacaa gccgcgtccg ggtcgcgtga aagactacat cgccattcca aaagcgaacg
    10921 gctatcagtc tttgcacacc tcgatgatcg gcccgcacgg tgtgccggtt gaggtccaga
    10981 tccgtactga agatatggat cagatggcgg agatgggtgt tgccgcgcac tgggcttata
    11041 aagagcacgg cgaaaccagc accaccgcgc aaatccgcgc ccagcgctgg atgcaaagct
    11101 tgctggagct gcaacagagc gccggtagtt cgtttgaatt tatcgagagc gttaaatccg
    11161 atctcttccc ggatgagatt tacgttttca caccggaagg gcgaattgtc gagctgcctg
    11221 ccggtgcaac gcccgtcgac ttcgcttatg cagtgcatac cgatatcggt catgcctgcg
    11281 tgggcgcacg cgttgaccgc cagccttacc cgctgtcgca gccgcttacc agcggtcaaa
    11341 ccgttgaaat cattaccgca ccgggcgctc gcccgaatgc cgcgtggctg aactttgtcg
    11401 ttagctcaaa agcgcgcgcc aaaattcgtc agttgctgaa aaacctcaag cgtgatgatt
    11461 ctgtaagcct gggccgtcgt ctgctcaacc atgctttggg tggtagccgt aagctgaatg
    11521 aaatcccgca ggaaaatatt cagcgcgagc tggatcgcat gaagctggca acgcttgacg
    11581 atctgctggc agaaatcgga cttggtaacg caatgagcgt ggtggtcgcg aaaaatctgc
    11641 aacatgggga cgcctccatt ccaccggcaa cccaaagcca cggacatctg cccattaaag
    11701 gtgccgatgg cgtgctgatc acctttgcga aatgctgccg ccctattcct ggcgacccga
    11761 ttatcgccca cgtcagcccc ggtaaaggtc tggtgatcca ccatgaatcc tgccgtaata
    11821 tccgtggcta ccagaaagag ccagagaagt ttatggctgt ggaatgggat aaagagacgg
    11881 cgcaggagtt catcaccgaa atcaaggtgg agatgttcaa tcatcagggc gcgctggcaa
    11941 acctgacggc ggcaattaac accacgactt cgaatattca aagtttgaat acggaagaga
    12001 aagatggtcg cgtctacagc gcctttattc gtctgaccgc ccgtgaccgt gtgcatctgg
    12061 cgaatatcat gcgcaaaatc cgcgtgatgc cagacgtgat taaagtcacc cgaaaccgaa
    12121 attaatgttt tatgaaccca acacgttatg cacgcatctg cgaaatgctc gccaggcggc
    12181 agcctgatct gaccgtctgc atggagcagg tccacaaacc tcataacgtt tctgcgatta
    12241 ttcgtaccgc agatgccgtt ggcgtacatg aagttcacgc cgtctggcct ggtagccgca
    12301 tgcgcaccat ggcttcggca gcggcgggta gtaacagctg ggtacaggtg aaaacacacc
    12361 gcaccattgg cgatgccgtc gctcatctga aaggccaggg tatgcagatt ctggcaaccc
    12421 atctttctga taacgctgtc gatttccgcg aaattgatta cactcgcccg acctgcattt
    12481 tgatgggaca ggagaaaacg ggcatcacgc aggaagcatt ggccctggcg gatcaggaca
    12541 tcatcattcc gatgatcggc atggtgcagt cgctgaatgt ttccgttgcc tcagccctca
    12601 ttctttacga agcccagcgt cagcggcaaa atgcaggcat gtacctgcgt gaaaacagca
    12661 tgttgccgga agcagagcaa caacgcctgt tgtttgaagg cggctatccg gtgctggcga
    12721 aagtcgcaaa acgcaaaggc ctgccttatc cccacgtcaa tcagcaaggc gagatcgaag
    12781 ctgatgccga ctggtgggct actatgcagg ctgcagggta agtgccatga aaggtcgcct
    12841 gttagatgct gtcccactca gttccctaac gggcgttggc gcagcactta gtaacaaact
    12901 ggcgaaaatc aacctgcata ccgtgcagga tctactctta caccttcccc tgcgctacga
    12961 agatcgcacc catctctacc ccatcggaga actactgccg ggcgtttatg ccacggtgga
    13021 aggcgaagtg ctgaactgca atatctcctt cggcggtcgg cggatgatga cctgccagat
    13081 cagcgacggt tccggcatcc tcaccatgcg ctttttcaat ttcagcgcgg caatgaaaaa
    13141 tagcctggcg acgggccgcc gtgtactggc ttatggcgaa gcaaagcgcg gtaaatatgg
    13201 tgcggagatg atccacccgg aataccgcgt gcagggcgat ctcagcacgc cagaattaca
    13261 ggaaacgctc acgccggttt atccaacaac ggaaggcgta aagcaggcca cgctgcgtaa
    13321 attaaccgac caggcgctgg atctgctcga cacctgcgcc attgaagaac tcctgccgcc
    13381 ggaactgtca caaggaatga tgacgctacc ggaagcgttg cgcactttgc accgcccgcc
    13441 accgacgcta cagcttagcg atctggaaac cgggcagcat ccggcgcaac gtcgtctgat
    13501 tctggaagaa ctgctggcgc acaacctcag catgttagcc ttacgtgccg gagcacagcg
    13561 ttttcatgcc cagccgctga gcgccaatga tgcgctgaaa aataaactcc tcgcagcctt
    13621 accgttcaag ccaacgggcg cacaggcacg cgtagtggcg gagatcgagc gcgatatggc
    13681 gctggatgtg ccgatgatgc gcctggtaca gggcgatgta ggttccggta aaacgctggt
    13741 cgccgccctc gccgcgctgc gtgcgattgc ccacggcaaa caggtggcat tgatggcacc
    13801 aaccgaatta ctcgccgaac agcacgccaa taacttccgc aactggtttg aaccgctcgg
    13861 tatcgaagtg ggctggctcg ccggtaagca gaaaggcaaa gcgcgactgt ctcagcagga
    13921 agccatcgcc agcggtcagg tgcagatgat tgttggtacg cacgccatct tccaggaaca
    13981 ggtgcagttt aacggtctgg cgctggtgat tatcgacgaa caacatcgct ttggcgtgca
    14041 tcagcgtctg gcgttatggg agaaaggcca gcagcaaggc ttccatccgc atcagttgat
    14101 catgaccgcc acgccgatcc cccgcacgct ggcaatgacg gcgtatgccg atctcgatac
    14161 ctcggtgata gatgaactgc cgccaggccg cacgccagtg actacggtcg ctattcctga
    14221 tactcgccgt accgacatca ttgaccgcgt gcgccacgcc tgcatgactg aaggtcgtca
    14281 ggcgtactgg gtttgtacgt tgattgaaga gtcagagtta ctggaagcgc aggcggcgga
    14341 agctacctgg gaagagttga aactggcgct accagaattg aatgttggcc tggtacacgg
    14401 gcggatgaaa ccagccgaga aacaggcggt gatggcgtcg tttaaacaag gtgagctaca
    14461 tctgctggtt gccactaccg ttattgaagt cggtgttgat gtgcctaacg ccagcctgat
    14521 gattatcgaa aaccctgagc gtctgggtct ggcgcagtta caccagctgc gcgggcgcgt
    14581 aggtcgtggc gcggtggctt ctcactgcgt gctactctac aaaacgccgc tttctaaaac
    14641 ggcgcaaatt cgcctgcaag tgctgcgcga cagtaacgac ggttttgtga ttgcgcaaaa
    14701 agatctggag attcgcggcc ctggcgaatt gttaggcacg cgtcagacgg gtaatgctga
    14761 atttaaagtg gcggatttac tgcgcgatca ggcgatgatc ccggaagttc agcgcctggc
    14821 acgccatatt cacgaacgtt acccacaaca ggcaaaagcc ctgatagaac gctggatgcc
    14881 ggagacggaa cgttactcga atgcgtaaaa ggcggcagtg agaagaccgc catttcaggt
    14941 taccctacct tcctgccgga tgcgattcat caccctacaa attcaataaa ttatgaatca
    15001 atacgcaggc ttgataagcg aagcgtatca ggcatttgtc tttgttgcgg cggcacttct
    15061 accgccgctt catcggttaa ccggcaaaaa tcggcaacat caaatacaac ttaattacca
    15121 gcgcattgac gatatcgata aagaacgcac cgaccatcgg caccaccaaa aacgccatgt
    15181 gcgacgggcc aaagcgttca gtaatcgcct gcatgttggc gattgccgtt ggcgttgcac
    15241 cgaggccaaa accacagtga cctgcagcca gcactgccgc atcgtagttt ttgcccatca
    15301 tgcgccaggt aacgaagatg gcatacaacg ccatgaagat ggtctgtacc accagaatcg
    15361 ccagcatcgg cagcgccagc gaagccagct cccacagttt cagccccatc aacgccatcg
    15421 ccaggaacaa cgacaagctt acgttaccca gcacggatac cgcacgctca aagacgcggt
    15481 aaaagcccat tattgacaga ccgttgctca gaatcacgcc aacaaacagt acacagacga
    15541 aggtcggcag ttcaaaagca gtgccagcca aaagttgcgc aacaattttc cccaccgtca
    15601 ggcagatagc aatcagcgcg atagtttcaa tcagcaccaa cgaggtgatc atgcgtccca
    15661 catccggctt ttcaaacgcc gtcgggactt cctggtcatc cggaataccg ttcggcgtgg
    15721 tggagtgttt caccagatag cgcgccaccg gaccgccaat caagccgccc agcaccagac
    15781 cgaacgttgc acaggccatc gccacttccg tcgcattggt gaagccataa cgttcaatga
    15841 acaatttact ccacgcagcg cccgtaccgt gaccgccgga aagcgtaata gaaccggcca
    15901 acagccccat cagcggatca agccctaaca ggctagccat accaatgcca atggcatttt
    15961 gcatcaccaa cagaccaaca accacaatca agaagatgcc aaccacacgc ccaccggcac
    16021 gcaaactggc aatgttggcg ttcaggccaa tggtggcgaa gaaagccagc attaacggat
    16081 cgcgcaggga catatcaaag ttgacttccc agcccatgct ttttttcagt actagtagcg
    16141 ccagcgccac caacaaacca cccgcaacag gttccggtat ggtgtatttc ttcaaaaagg
    16201 agacggaatg gaccaactta cgcccgagca gcaacgtcag cgttgcggca acaagcgttg
    16261 ctaaagtatc gagatgaaac atagttactc ctttgtatcc gcatcactct ttcatactcg
    16321 tcatacttca tcgcgcagtg gcaacttcat gaactatgac gggtatcgag ttatgctttc
    16381 ctgggtcaaa ttctcagctg ttaatgaaca aatggcatgg attttaagca gaaaaatacc
    16441 aaaagttata taaaaaagtg catttatctc atttaaatat ttagatgagg atgtggcaaa
    16501 cgtttgcttt tctctttcgg tcagctaaaa tgcccgcttt gctaccacgg gattgttttc
    16561 gatgtctgtt tccaccctcg agtcagaaaa tgcgcaaccg gttgcgcaga ctcaaaacag
    16621 cgaactgatt taccgtcttg aagatcgtcc gccgcttcct caaaccctgt ttgccgcctg
    16681 tcagcatctg ctggcgatgt tcgttgcggt gatcacgcca gcgctattaa tctgccaggc
    16741 gctgggttta ccggcacaag acacgcaaca cattattagt atgtcgctgt ttgcctccgg
    16801 tgtggcatcg attattcaaa ttaaggcctg gggtccggtt ggctccgggc tgttgtctat
    16861 tcagggcacc agcttcaact ttgttgcccc gctgattatg ggcggtaccg cgctgaaaac
    16921 cggtggtgct gatgttccta ccatgatggc ggctttgttc ggcacgttga tgctggcaag
    16981 ttgcaccgag atggtgatct cccgcgttct gcatctggcg cgccgcatta ttacgccgct
    17041 ggtttctggc gttgtggtga tgattatcgg cctgtcgcta attcaggtgg ggctgacctc
    17101 cattggcggc ggttacgcag ccatgagcga taacaccttc ggcgcaccga aaaatctgct
    17161 gctggcaggc gtggttttag ccttaattat cctgcttaac cgtcaacgta acccttactt
    17221 acgcgtggcc tcactggtaa ttgcgatggc ggccggatat gcgctggcgt ggtttatggg
    17281 catgttgcca gaaagcaacg aaccgatgac gcaagaactg attatggtgc caacgccgct
    17341 ctattacggt cttggcattg aatggagtct gctgctgccg ctgatgctgg tctttatgat
    17401 cacttcgctg gaaaccattg gcgatatcac ggcgacctct gacgtttccg aacagccagt
    17461 gtccggtccg ctgtacatga aacgcctgaa aggcggcgtg ctggcaaacg gcctgaactc
    17521 gtttgtttcg gcggtgttta acaccttccc gaactcctgc ttcgggcaaa acaacggagt
    17581 gatccagttg accggtgttg ccagccgcta tgtcggtttt gtcgtcgcgc tgatgttgat
    17641 cgtgctgggt ctgttcccgg cagtgagcgg ttttgtacaa cacattccag aaccggttct
    17701 gggcggcgca acgcttgtaa tgtttggcac catcgccgcc tccggtgtgc gtatcgtttc
    17761 tcgtgagccg ctgaaccgtc gggcgattct gattatcgcg ctgtcgctgg cggttggtct
    17821 gggcgtgtct cagcagccgc tgattttgca gtttgcccct gaatggctga aaaacctgct
    17881 ctcctccggg atcgccgcgg gcggtattac tgccatcgtg ctgaatctga ttttcccacc
    17941 agaaaaacag taattcactg gcggcggtaa gataattacc gccgcagtct cacctcttta
    18001 accattcccg ccttgaggat tgcgcgtaaa tcgtgcataa ctcccttatg tgcatttcac
    18061 gggatggaag accatgaaat ttattgggaa gctgcttctc tacattctca tcgctctgtt
    18121 agtggcgatc gctggcctct attttcttct gcaaacccgc tggggagcag aacatatcag
    18181 cgcatgggtt tccgagaata gcgactatca tctggccttc ggggcgatgg ttcaccgttt
    18241 ttccgcgcca tctcatatcg tgctggagaa tgtcacgttt ggtcgtgatg gccagcccgc
    18301 gaccctggtg gccaaaagtg tcgacattgc gctaagtagt cggcaactga ccgaaccacg
    18361 ccatgtcgat accatcctgc tggaaaacgg gacgctgaat ctcaccgacc agaccgcgcc
    18421 gctaccgttc aaagccgatc gtctgcaact gcgtgatatg gcgtttaata gcccgaatag
    18481 cgaatggaaa ctgagcgcgc agcgggtaaa tggcggcgtg gttccgtggt caccagaagc
    18541 cggtaaagtg ctgggtacga aggcgcagat tcagtttagt gccggatcgc tttcgctcaa
    18601 tgatgttcct gccaccaatg tactgattga aggcagtatt gataacgatc gcgttacgct
    18661 gactaacctg ggtgccgaca tcgcgcgcgg gacattaacc ggaaacgcgc agcgtaacgc
    18721 cgacggcagc tggcaagtgg aaaacctgcg catggcggat atccgtctac aaagcgaaaa
    18781 atcgctaacc gacttctttg cgccattacg ctctgtcccg tcgttgcaga ttggtcgcct
    18841 ggaagtgatc gatgctcgtt tgcaaggtcc ggactgggcg gtgaccgacc tcgatctcag
    18901 cttgcgcaac atgaccttca gtaaagatga ctggcagaca caagaaggca aactgtcgat
    18961 gaacgctagc gagttcattt atggttcgct gcatttattt gacccgatta taaacgcgga
    19021 attttctccg cagggcgtag cgctgcgcca gttcaccagc cgctgggaag ggggtatggt
    19081 cagaacgtca gggaactggc tgcgtgacgg gaaaacgttg atccttgatg atgcggcaat
    19141 tgccggactg gaatatacct tgccgaaaaa ctggcaacag ttgtggatgg aaacgacacc
    19201 cggttggtta aacagcctgc aactgaagag atttagcgcc agccgcaatc tgatcattga
    19261 tatcgaccct gacttcccgt ggcagctcac cgcgctcgat ggttacggtg ccaacctgac
    19321 gctggttacc gatcataaat ggggcgtctg gagtggctcg gcgaatctga atgccgccgc
    19381 cgcgacgttt aatcgtgttg atgtccgtcg tccgtcgctg gcgctaaccg ccaacagcag
    19441 cacggtgaat atcagcgaac tgagtgcatt tactgaaaaa ggcattctgg aagccaccgc
    19501 cagtgtttca caaacgccac aacgtcagac acatatcagc ctgaatggac gcggtgtgcc
    19561 ggtgaatatt ttgcaacagt ggggatggcc tgaattaccg ttgactggcg acggcaatat
    19621 tcagcttacc gccagtggcg atattcaggc caatgtcccg ttgaaaccta cggttagcgg
    19681 gcaattgcat gccgtgaacg ccgcaaagca gcaagtgact caaaccatga atgcgggcgt
    19741 cgtttccagc ggtgaagtta catcggcaga gctggtgcag taaacatcaa tctcgccggg
    19801 tacgactcag acgtacccgg cattcttcaa tagattacaa cgtaattgtc agcgcattcc
    19861 cttgaggcgt aaccaccagc ccctgctcac tttcagcctg cgaaccgcct tgcagaccat
    19921 ttactttcac aatattgcgc aagcacagtg tccagttcct cgcctcgccc gcaccagtca
    19981 cagtaatcgt gttaccagta cgtgttgctt ttagagtaaa gatcaccgaa ccgtcagcag
    20041 cgggcacttc acatacagct tcatgcccgt cttgcagatt aaagagatgg aatgccgtgc
    20101 cttcgtgcca cgcgtaatcg ggacgttgat cgttgttgcc cagcgccaat agggtgttat
    20161 cacgcacata aacgggcaga ctcaggaagc cgtgctgctg tttatgccag cgactaccgt
    20221 cgagctcatt gttgtgccac aggtgtgtcc agcgaccttc cggcaagtag aactgcacct
    20281 cgcccgcctc agtgaacacc ggagcaacca tcacgttgtc gcctagcatg tattgacggt
    20341 caaggtaatc acaagccgga tcgtccggga actccatcat catggcccgc atcatcggcg
    20401 taccccgcgc tttcgcacgc gcagcttcac gatacagata cggcatcatg cggcatttca
    20461 gttgcgtgaa gaagcgcacc acatcacagg actcatcatc gtaggcccac ggcacacgat
    20521 aagatttgct gccatgtaaa cgactatggc tggagagcaa accaaacgcg caccagcgtt
    20581 tgtaaacgtg tgccggcgcg gtattttcaa agccgccgat atcgtggctc cagaagccaa
    20641 aacctgaaag gccaatagac aaaccaccgc gcaggctttc cgccattgat tcgtagttag
    20701 cgtaacaatc gccaccccag tgtaccggga atttctgcgc accgacggag gccgagcggg
    20761 caaacaagac agcttcttcc tcaccaacgg tgtccttgag cacgttccac accagttcgt
    20821 tgtagatgta cgcataatgg ttatgcattt tctgcggatc ggaaccgtca aaccactgaa
    20881 catcagttgg gatacgttcg ccaaagtcgg tcttaaagca atcaacgccc atcgcgacca
    20941 gacctttcag tttgtcggcg taccatttgc aggcatccgg attggtaaag tcataaatcg
    21001 ccaga
//
LOCUS       DAEEKQ010000060        20880 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.060, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000060 DAEEKQ010000000
VERSION     DAEEKQ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20880
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.060"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..384
                     /locus_tag="KLK57_002134"
     CDS             <1..384
                     /locus_tag="KLK57_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBE0892498.1"
                     /translation="VQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLLAQAIKQSI
                     RKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRLQHIAPQKEIGFSSGIYAMKEN
                     DTLHDAYKASDERLYVNKQNKNSRS"
     gene            427..930
                     /gene="yeaK"
                     /locus_tag="KLK57_002135"
     CDS             427..930
                     /gene="yeaK"
                     /locus_tag="KLK57_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HBE0892499.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(931..1035)
                     /locus_tag="KLK57_002136"
     CDS             complement(931..1035)
                     /locus_tag="KLK57_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892500.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            1205..1651
                     /locus_tag="KLK57_002137"
     CDS             1205..1651
                     /locus_tag="KLK57_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HBE0892501.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(1608..2429)
                     /gene="nimR"
                     /locus_tag="KLK57_002138"
     CDS             complement(1608..2429)
                     /gene="nimR"
                     /locus_tag="KLK57_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HBE0892502.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKGPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            2526..3707
                     /gene="nimT"
                     /locus_tag="KLK57_002139"
     CDS             2526..3707
                     /gene="nimT"
                     /locus_tag="KLK57_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HBE0892503.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFIGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLATPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMIVPLA
                     LNGFGWQGALLMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLIYYVIIGWLPAILISHGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            3762..4109
                     /locus_tag="KLK57_002140"
     CDS             3762..4109
                     /locus_tag="KLK57_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HBE0892504.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4131..4385)
                     /gene="yoaF"
                     /locus_tag="KLK57_002141"
     CDS             complement(4131..4385)
                     /gene="yoaF"
                     /locus_tag="KLK57_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HBE0892505.1"
                     /translation="MKIISFALPCLLVLAGCSTSSQPEVPKPPQIGMANPASVYCQQK
                     GGTLIPVSTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            4568..5593
                     /gene="dgcP"
                     /locus_tag="KLK57_002142"
     CDS             4568..5593
                     /gene="dgcP"
                     /locus_tag="KLK57_002142"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HBE0892506.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGISTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNPRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHSAPHC"
     gene            5626..5724
                     /locus_tag="KLK57_002143"
     CDS             5626..5724
                     /locus_tag="KLK57_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HBE0892507.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            5727..5801
                     /gene="yoaJ"
                     /locus_tag="KLK57_002144"
     CDS             5727..5801
                     /gene="yoaJ"
                     /locus_tag="KLK57_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HBE0892508.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(5860..6108)
                     /locus_tag="KLK57_002145"
     CDS             complement(5860..6108)
                     /locus_tag="KLK57_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBE0892509.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(6256..6438)
                     /locus_tag="KLK57_002146"
     CDS             complement(6256..6438)
                     /locus_tag="KLK57_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HBE0892510.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYDTENEHDVCGW"
     gene            complement(6442..6801)
                     /gene="yeaR"
                     /locus_tag="KLK57_002147"
     CDS             complement(6442..6801)
                     /gene="yeaR"
                     /locus_tag="KLK57_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HBE0892511.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFEVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            complement(6974..7612)
                     /gene="leuE"
                     /locus_tag="KLK57_002148"
     CDS             complement(6974..7612)
                     /gene="leuE"
                     /locus_tag="KLK57_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HBE0892512.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            complement(7739..8662)
                     /gene="dmlR"
                     /locus_tag="KLK57_002149"
     CDS             complement(7739..8662)
                     /gene="dmlR"
                     /locus_tag="KLK57_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HBE0892513.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITIEFLRQYFAEHYPNFSLEHA"
     gene            8765..9850
                     /gene="dmlA"
                     /locus_tag="KLK57_002150"
     CDS             8765..9850
                     /gene="dmlA"
                     /locus_tag="KLK57_002150"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HBE0892514.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFDLSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGSATTPQVADAICKIILR"
     gene            10101..11711
                     /gene="yeaV"
                     /locus_tag="KLK57_002151"
     CDS             10101..11711
                     /gene="yeaV"
                     /locus_tag="KLK57_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HBE0892515.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANTIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISTWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYPPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            11743..12867
                     /gene="yeaW"
                     /locus_tag="KLK57_002152"
     CDS             11743..12867
                     /gene="yeaW"
                     /locus_tag="KLK57_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HBE0892516.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            12923..13888
                     /gene="yeaX"
                     /locus_tag="KLK57_002153"
     CDS             12923..13888
                     /gene="yeaX"
                     /locus_tag="KLK57_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HBE0892517.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALNEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(13942..15057)
                     /gene="rnd"
                     /locus_tag="KLK57_002154"
     CDS             complement(13942..15057)
                     /gene="rnd"
                     /locus_tag="KLK57_002154"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HBE0892518.1"
                     /translation="MITTDDALASLCEAVRVFPAIAQDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(15139..16824)
                     /gene="fadD"
                     /locus_tag="KLK57_002155"
     CDS             complement(15139..16824)
                     /gene="fadD"
                     /locus_tag="KLK57_002155"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HBE0892519.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLIDMFEQSVVRYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCCRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(17029..17610)
                     /gene="yeaY"
                     /locus_tag="KLK57_002156"
     CDS             complement(17029..17610)
                     /gene="yeaY"
                     /locus_tag="KLK57_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HBE0892520.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MNAPDLYVGQEARFGGKVVVVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGTVDGKVNNAPYKFMVMQVTGYKRWHITQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVETIVTE"
     gene            complement(17650..18345)
                     /gene="tsaB"
                     /locus_tag="KLK57_002157"
     CDS             complement(17650..18345)
                     /gene="tsaB"
                     /locus_tag="KLK57_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HBE0892521.1"
                     /translation="MRILAIDTATEVCSVALWNDGNVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWRGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLRKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(18403..20313)
                     /locus_tag="KLK57_002158"
     CDS             complement(18403..20313)
                     /locus_tag="KLK57_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HBE0892522.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGCAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDLLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            20445..20789
                     /locus_tag="KLK57_002159"
     CDS             20445..20789
                     /locus_tag="KLK57_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HBE0892523.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         5091 a         5389 c         5115 g         5285 t
ORIGIN      
        1 gtgcaatccg gttcttcggt gatgtttatt gcaattgaca tggacaagtt aaagcaaata
       61 aatgacaccc tcggtcatca ggagggggat ttagcgatta cgttattagc tcaggcgatt
      121 aaacaatcga ttcgtaaaag tgattatgcc atccgactcg gtggcgatga attctgcatc
      181 attcttgtcg attcgacgcc gcaaattgca gcacaactgc ctgaacgtat cgaaaaacgt
      241 ctgcaacata tcgcgccgca gaaagaaatc ggcttctctt ccggtattta cgcgatgaaa
      301 gaaaacgata cgttacatga tgcgtataaa gcttccgatg agcgtttata tgtcaataag
      361 cagaacaaaa acagccgttc atgataacct tctgtggttg tttgcttgta atctcaggag
      421 cgtgaaatga ctgaaatggc taaaggaagc gtgacgcatc agcggttaat cgctttatta
      481 tcacaagaag gtgctaactt ccgcgtcgtt acccatgaag cggtaggtaa atgtgaagcg
      541 gtgtcagaaa ttcgtggcac cgctttaggc cagggagcta aggcactggt atgtaaagtc
      601 aaaggcaatg gcgtaaatca acatgttctg gcgatcctcg ctgccgatca acaggccgat
      661 ctgagccaac ttgccagcca tataggcgga ttacgcgctt cgttagccag ccctgctgaa
      721 gtcgatgaac tcacaggctg tgtcttcggc gcgatccccc ctttcagctt ccatccaaaa
      781 ctcaaactgg ttgccgaccc tctactcttc gaacgatttg atgaaatcgc cttcaatgca
      841 gggatgctgg ataaatccgt tattttgaaa accacggatt atttgcgcat tgcgcaacca
      901 gaactgctca atttccgccg cactgcgtaa ctagcctgtc cgttcaataa gcagaacgga
      961 caaaaccagc acaacagcaa tggcaaaaaa cgatgacgtg ataatcagtg tctcgacgaa
     1021 catttgatcg ttcatagcat gcccccggga gatagctgtt ttcacgttat tgttagcgtg
     1081 cacaaatggc agtttgatga caattcgcct gattttttat gcaaaaaacg taaagatttt
     1141 ctacttcctt cctgcagcaa acgtaaagta agcaggctta ttattttttg gcaaggaaac
     1201 cacgatgttt gatgtcactt tgctgatcct gctcgggtta gctgcgctgg gctttatcag
     1261 tcataacacc actgtcgccg tttcaattct ggtgttgatc attgtccgcg tcacaccgtt
     1321 aagcaccttt ttcccgtgga ttgaaaaaca ggggctaagt atcgggataa tcattctgac
     1381 gattggcgtt atggccccca tcgccagcgg gacgctaccg ccctcaacct tgattcactc
     1441 gtttctgaac tggaaatcgc tggtggcaat tgcagtaggc gtgattgtct cctggctggg
     1501 tgggcgcggc gtgaccttaa tgggcagcca gccgcaactg gtcgccgggc tgctggtagg
     1561 cactgtttta ggcgtggcgc tgtttcgcgg cgtaccggtc ggcccgctta ttgccgcggg
     1621 tctggtttcg ctgattgtgg ggaaacagta gttaatcccg caatatatcg gcctggcgtt
     1681 tgccccaggc ctttcttaaa catagtaata aaggccgttg ttgaatcata ccccagcgta
     1741 tgagcaactt tctgtacagt atcgcctttc actaatccct gaagtgccat aatcagctgt
     1801 aactgctgac gccactggcg aaaactcaac cccgtctctt ttactatcag tcgcgcaaga
     1861 ttgcgttcac tcatcgcaaa aaagccagcc cattgcccta atgaccccca ctcgacgggc
     1921 cctttcgcca tcatctccac catcgtgcgg attttaggat gagaagaaac gggcaggtgc
     1981 agctgttgtt gtggctgctg agggagttca tcaaacagga cctgaattaa tctgcgggtc
     2041 atcggctcag cacgctgagt cgtcgtcctg tttgccaggg ttaaaatcaa ttcccggcat
     2101 aagggcgata ttttcaatgt gcaacatgtt gtcggcattg tcactgcgct gggttcgata
     2161 aacagaaaac agagttcagc gttagctgtc acttgattgc tatgttccac cccgcccggt
     2221 atccaaaccg catattgcgg cggcaccatc cataaagcat tttccaccgt acaggtaatt
     2281 gcaccatgta atgctagtat cagctgtcct ttgcggtgct gatgtacagg actggttaac
     2341 tcatccgttc cggcgtgaat acaaaatgcg accgcggctt catggtgtcg gtcgggttca
     2401 tagccattga gattcaacct gtgcatcatt ttgtccgaat ttagcgataa tttgtcattt
     2461 tagcttgatt caacataaca ataaaaacgg taaggtacag cctcgtttgt aacaatgaga
     2521 agcatatgac ctgttcaact tcattaagcg gcaaaaacag gattgtcctt atcgctggca
     2581 ttctgatgat tgccacaaca ttacgcgtca cctttatcgg cgcagcaccg ttactggata
     2641 cgattcgttc cgcttactcg ctgacgacag cgcaaaccgg cttattgacc accctgccat
     2701 tattggcctt tgcgctaatc tcacctttgg ccaccccggt agcgcgacgt tttggtatgg
     2761 aacgtagcct gtttgccgcg ttacttttga tctgtgctgg tatcgcaatt cgctctctcc
     2821 cttcgcctta cttattattt ggcggtacag cggtcattgg cggtgggatt gcattaggca
     2881 atgtcttact gccaggatta attaaacgcg atttccctca ttccgtcgcc agacttaccg
     2941 gcgcatattc cctgacaatg ggagctgcag cggcactggg atcggctatg atagtgccgc
     3001 tggctttgaa cggttttggc tggcaaggcg cgttgctcat gctgatgtgt tttcctctgc
     3061 tggctctttt tttatggctg ccacagtggc gaagccaaca acatgcaaat ttgagtacct
     3121 cgcgcgcctt acatactcgg ggtatctggc gttcgccgct tgcctggcag gtaacattgt
     3181 ttcttgggat caactcactg atctattacg tgattattgg ctggcttccg gcgatcctca
     3241 tcagtcacgg ctatagcgaa gcacaggcgg gttcactgca tggtttgctg caactagcca
     3301 cagcagctcc cggtttgctg atccctcttt tcttacatca tgtgaaagat cagcgtggta
     3361 ttgcagcgtt cgttgccttg atgtgcgcag tgggcgctgt tgggctctgc tttatgccag
     3421 cgcacgcgat cacctggact ctgcttttcg gttttggttc cggcgcaaca atgatactgg
     3481 ggttgacgtt cattggtctt cgggcaagtt ctgcgcatca ggcggcggca ctctcgggga
     3541 tggcacaatc cgtcgggtat ttgctggcag cctgtgggcc gccgctaatg ggtaaaatac
     3601 acgatgctaa cggtgactgg tccgtaccac tgatgggtgt tgcaatactt tcactactga
     3661 tggcgatttt cggactttgc gccgggagag acaaagaaat tcgctaatat ccggtgctat
     3721 agtgacgtga caaatcatgc gtgaaaggga gaacaaacac tatgaatatt cagtgcaaac
     3781 gcgtttatga tccggctgaa cagagcgatg gttatcgcgt actggtcgac cgcctctggc
     3841 cgcgcggtat caaaaaaacc gatttagccc ttgatgagtg ggataaagaa atcacgccgt
     3901 caacggaact gcgcaaagcc tttcacggcg aagtcgtcga ttttgcaacc tttcgcgagc
     3961 aatatcttgc agaactggcg caacacgagc aagaaggaaa gcggctggcg gacatcgcca
     4021 aaaaacagcc gctgaccctg ctctacgcag caaaaaacac cacgcagaac catgcgctgg
     4081 tgctggccga ctggctacgt agcttgtgat tttagtacag catccggcgg ttatttttca
     4141 ccagccggat ggtcccgtcg ccacaatgcc cattcatcaa tcgtttcacc gcccggtaat
     4201 ttgcaattgt tgctgacccc ttgcgctgtc gacactggaa tgagcgtccc gcccttctgc
     4261 tggcaataga ccgacgccgg atttgccata ccaatctgcg gcggtttagg tacttctggc
     4321 tgagaagagg ttgaacaacc agccaggacc agcaagcaag gcaaagcaaa actgataatt
     4381 ttcatttatt gatctcactc atttatctac gcttagagta tcgcggtatc gttttgtttt
     4441 gcagcactat ttttattaca ttcactcaaa acatattacg tcttgtttca tctttgttgg
     4501 tgatgtttta tcatgcctgc aaagattaaa taatcagcat ttacccgccg tatcctggag
     4561 ttgtttcgtg tcagatcaga ttatcgcccg cgtctcgcaa tcccttgcca aagaacagtc
     4621 actggaaagt ctggtccgac agcttctgga gatgctggaa atggtcactg atatggaatc
     4681 aacctacctg accaaagtgg atgtcgaagc gcgcctgcag catattatgt ttgcccgtaa
     4741 cagccagaaa atgcacatcc cggagaattt taccgtctcg tgggattact cgttatgcaa
     4801 acgcgccatt gatgaaaact gctttttcag cgatgaagtc cctgaccgtt ggggcgactg
     4861 tattgcggca cgcaatcttg gcatctccac atttctgagc acaccaattc acttaccgga
     4921 tggatcattc tatggcacgc tttgcgccgc cagcagtgag aagcgccagt ggagtgaacg
     4981 cgcggaacag gttttacagt tattcgccgg actgattgca caatatattc aaaaagaggc
     5041 actggttgaa cagttgcgcg aagccaatgc tgcgctgatt gcgcaatcgt ataccgactc
     5101 gttaaccggg ttgccgaatc cgcgggcgat ttttgaaaat ctgacgacgc tgttttctct
     5161 cgcccggcat cttaaccata agataatgat cgcgtttatc gatctggata acttcaaatt
     5221 aatcaatgat cgttttggtc ataatagtgg cgatctgttt ctcattcagg ttggcgagcg
     5281 ccttaatacg ctccagcaaa atggcgaagt tattggtcgt ctcggcggtg atgagttttt
     5341 ggttgtttca ctaaacaacg agaatgcgga tatttcgtcg ctgcgagaac gcattcagca
     5401 gcaaatacgt ggagaatatc acttaggtga tgttgatttg tattatcccg gtgccagtct
     5461 tggcatagta gaagtcgatc ctgaaacaac cgatgcagac agtgccctgc atgctgccga
     5521 tattgcgatg tatcaggaga aaaaacacaa acagaaaaca ccttttgtcg cgcattcagc
     5581 gccacattgc tgaggcgtat tcacatcctt ttgattggtg ataacatgcg aatcggtatt
     5641 atttttccgg ttgtaatctt tattacagcg gtcgtatttt tagcatggtt ttttattggc
     5701 ggctatgctg ccccgggggc ataaagatga aaaaaacaac gattattatg atgggtgtgg
     5761 cgattattgt cgtactcggc actgagctgg gatggtggta acgtcacctc taaaaaatag
     5821 caaaggctgc ctgtgtgcag cctttgtgca atttaagcgt taacttttaa tcttcctgta
     5881 gataaatagc acgacaatcg caccaataac ggcaaccacg aagctgccaa aattgaagcc
     5941 atcgacttta ccaaagccaa acagcgtgct gatccatccg ccgactacgg caccgactat
     6001 ccccagcagg atagtcataa agaatccacc accatcttta cctggcatga tccacttcgc
     6061 cagaataccg gcaataagcc caaaaataat ccatgacaga atgcccattg tttcctcact
     6121 tatctgtttt gcattagcgg gttagtcgct gataaaaagc atagcacaac atcgggaggg
     6181 caagatttgt gacgagcatc acggaggatt tttttgcgat ggcgcagaaa ttgcgccatc
     6241 aacgatcagt gataattacc aaccacaaac atcatgttcg ttttccgtgt cataagaacg
     6301 cacggtattc accagatctt ttatcacttc agccgccact tctggcacca gcaaagtcat
     6361 cggcgtctct gtttcataat cgacagaaac gccattactg ttattggtga cggtcacggt
     6421 atacgttgct ttgcccatga ttcatttccc gttatgaatg actttccgtt gttgcgcacc
     6481 ttccatcagg acttcaggag ccacgaagaa gtcaatgttg aaataagtat cgtcagtcat
     6541 ggcttcaatg ttgtgccact tttctggagg gaacacctca aactgccccg cttcgataag
     6601 gatcacctga tcaggctctg cactgtgttc atcagcgtag ccgagatatt tgaccgcccc
     6661 atgcataacg gaaaggcgtg ggtaaacccc cgggcgcgtt cctttatcaa gatgacgttc
     6721 gaatattccg gcaggcgcag tttgtttatt ccagaaaggc gttgagcgcg tatgaatata
     6781 attctgtggg atttgcagca tccttttccc tccttcggtg aatgcgctga aaacggttta
     6841 ttccagccgt ttcagggtac gcctgataat ttgcatttta aataccattt attggttact
     6901 ttttagcacc atatcagaga aaaatcaggg aggattacaa gtgggaagcc cgcgacaacg
     6961 cgggctgaat gtatcaggat tgcagcgtcg ccagtcgggc ggcgaaaccc acgaacatca
     7021 aaccaatcag tgagttgcca actttagcca gtttcttttt ggtacgtatg tactgcgtga
     7081 caaaagcacc agaaataatc aggaagctca aatagcagaa actcaccagt tccagcgtcg
     7141 tcgccagaat aaagaatgaa attcccgtat gtggggcatt aacatcgata aactgtacga
     7201 aaaacgacac atagaacaaa atggctttcg gattagtcag gctcaaaatt aacgcgcgtt
     7261 taaaaatggc cccgtattgg ggctcatcgg atttggcctc gttattttta cccttcaggg
     7321 tcgcgtagag aattttactc cccagataga gcaaataaaa cgcaccaaga taacgtacaa
     7381 tgttgaataa tatcggggtg gtcttaatta atgtcgccac tccagcccat gccagaaaca
     7441 tcaatactgc atcgccaata aatacaccgc aggccgcaag ataaccgcct ttcataccgc
     7501 tactgacgct atttttgagt acaaacaggg tatttggccc tggcaccaac acaataaaaa
     7561 tggccccaac cagataggtc cagtaattca gaaccccgta ttcagcgaac acattaacct
     7621 ctttaattat ctttcgatca tgcgcgatta aaggtgaata tgctaaccaa tctgtagcgg
     7681 cttagaaagg agaaaatcag gttttaaccc gatatcaacc cgataattga ataattaatc
     7741 aggcatgctc cagtgaaaaa ttcgggtagt gctcggcaaa atactggcgt aaaaattcta
     7801 tcgttatccg cactttcgct gacgtcgcca gccttgaaac ataaacggcc cagacgttcg
     7861 ctggctggta atattccggt agcacttgca ctaaatgacc actggcaatg ttttcgctaa
     7921 catcccacca ggagcgcaga gcaatcccct gcccatcgag acaccactga tggacaattt
     7981 ctccatgatt agacgacagt gggccggtga ctttaatcgc atggtggcct tctttattgc
     8041 gtaattgcca gacaccaaaa ggatgatcgc gctctttaat gaccaggcac ggcaatgcgg
     8101 aaagatcggt taaatgtttc ggcgcgccat gttgcgcaat aaattccggc gacgcgcaga
     8161 gaatgcggta attagtcgcc agtttgcggg cgattaaatt aggggcgatg tcatcgccaa
     8221 tgcgaatatc gagatcaaca ccttcattga ccaaatcgac caaccggtct tccacatcaa
     8281 aacgtaattc cagttgcgga tacgccttag ccagcgctga taacgccgga gccaccacct
     8341 gtcgtccaaa accaaagctg ctgataatac gcagcatccc ctgcggcacc tgacgcacgt
     8401 cagaaagttc gtccatcatc tgaccgacat cctgcaaaat ccgctgcgcc cattcataaa
     8461 tccgctctcc ttcttcggta atggtgacgc ggcgggtggt gcggtgtaac aacaccacgt
     8521 ttagcgtttg ctccagcaag gcgatgcgct tgctgacgaa cgccggtgaa acgcccagtt
     8581 cttccgccac ggcggcaaaa ccagcccggc gagccaccag cataaagacg cgcaaatcgt
     8641 tcagcagcgg taaattattc atgattcgtg ttttatgttt caccagttac ggggattaat
     8701 tccttttcag tcaattatag gatggtgatg ttgtcaattt tgatggtcag gaagtgagaa
     8761 cccaatgatg aaaacgatgc gtattgctgc gatcccggga gatgggattg gcaaagaagt
     8821 ccttcctgaa gggattcgcg tattacaggc tgccgctgag cgctggggct tcgacttgag
     8881 ttttgagcaa atggagtggg cgagctgcga gtattacagc catcacggta aaatgatgcc
     8941 ggacgactgg catgagcaac ttagccgttt cgacgccatc tattttggtg ccgtcggctg
     9001 gccggatacc gttccggacc atatttcgtt gtggggttcg ctgctgaaat ttcgtcgtga
     9061 attcgaccag tacgtcaacc tgcgcccggt tcgtctcttt cctggcgttc cctgcccgct
     9121 ggcgggaaaa cagcctggcg acatcgattt ttacgtggtc agggaaaaca ccgaaggcga
     9181 atattcctcg ctcggcggta gagtgaatga aggtacagag catgaagtcg tcattcagga
     9241 atcggtattt acgcgtcgtg gtgtcgatcg cattttgcgt tatgccttcg aacttgcgca
     9301 aagccgccca cgtaagacgc taacttctgc cactaaatca aacggtttag ccatcagcat
     9361 gccgtactgg gatgagcgag tggaagcaat ggccgagaat tacccggaga tccgctggga
     9421 caagcagcat attgatattc tctgcgcgcg ttttgtgatg cagccggaac gcttcgatgt
     9481 ggtggtggcg tccaatttgt ttggcgatat cctttccgat cttggcccgg cctgcaccgg
     9541 caccattggc attgccccat ccgccaacct gaatccggaa cgcactttcc cgtcgctctt
     9601 cgagcctgtc cacggttccg cgccggatat ctacgggaaa aatattgcta accctatcgc
     9661 cacaatttgg gccggtgcga tgatgctcga ttttctcggc aatggcgatg agcgtttcca
     9721 gcaagcgcat aacggtattc tggcagcgat tgaagaagtg attgctcacg ggccgaaaac
     9781 accggatatg aaaggcagtg ccaccacgcc acaggttgcc gacgcgattt gcaaaattat
     9841 tttgcgttaa ggtcaaacca gtttatttga accgcgtcac tgacgcggtt tttttattcg
     9901 ttctttgcag ttaataacct gcgtcatttc accttttatt gtttccgttt cgtgttttat
     9961 ggctttccgt attcttaatt gtttaattta tgtaacatgc aaataatcaa tttgttaaac
    10021 acaacataca actcattacc ctgcaaataa ttacaactgg caaaagccag agacgggctt
    10081 ttatgcccag gagatgactc atgatgagca atgttaagaa aaaagatgtg ccgctgataa
    10141 gcatcagcct ggtggccatt cttttcatcg cagctgcatt aagccttttc ccacaacaat
    10201 cggccgacgc ggccaacaca atatacactt ttgttacgcg tacgttaggt tccgccgtac
    10261 aggtattggt tttgctggca atgggactgg tgatttattt agccaccagt aaatacggca
    10321 atattcgtct tggcgaagga aaaccggaat acagcacgct ctcctggctg tttatgttta
    10381 tttgtgccgg tttaggttct tctacgcttt attggggggt tgctgaatgg gcctattatt
    10441 atcaaacgcc tggattaaat atcgcaccac gttcacaaca ggcactcgaa tttagcgtcc
    10501 cttactcctt cttccactgg ggcatcagca cctgggcaac ttatacgctg gcctcattaa
    10561 tcatggctta tcactttcat gtgcgtaaaa acaaaggtct gagtctttcc ggcattattg
    10621 ccgcgattac cggcgttcgc ccgcaaggcc catggggaaa actggttgat ttgatgtttc
    10681 tgatcgccac tgtcggcgca ctgactattt cccttgttgt caccgcagca acctttactc
    10741 gtggactttc cgcgctgacc ggtttacccg ataacttcac cgtgcaggca tttgtgatcc
    10801 tgctttccgg cggcattttt tgcctaagct cgtggattgg tatcaacaac ggtttgcaac
    10861 gtctgagcaa aatggttggc tggggcgcgt tcctgctgcc attactggtg ctgattgtcg
    10921 gtccaaccga atttattacc aacagcatca tcaatgccat cggcctgacc acgcaaaact
    10981 tcctgcaaat gagcttattc accgatccgc ttggcgatgg ttcatttacc cgcaactgga
    11041 ccgttttcta ctggctgtgg tggatctcat acccccctgg cgtagcaatg tttgtcaccc
    11101 gcgtttcccg cggtcgtaag attaaagaag ttatctgggg actgatcctc ggcagcaccg
    11161 tcggttgctg gttcttcttc ggcgtaatgg aaagctatgc cattcatcag tttatcaatg
    11221 gcgtaatcaa cgtcccacag gtgctggaaa cactgggcgg cgaaacagct gtacagcaag
    11281 ttctgatgtc gttgccagcc ggtaaattgt tcctcgccgc atacctgggc gtgatgatta
    11341 ttttccttgc ctcgcatatg gatgcagtgg cctacaccat ggcggcgacc agtacgcgta
    11401 atctccagga aggtgacgat cctgaccgtg ggctgcgtct tttctggtgc gtggtgatca
    11461 ctctgatccc gctttccatc ttgtttaccg gtgcttcgct ggaaacgatg aaaaccaccg
    11521 tcgtgctcac agcccttccc ttcctcgtca ttttactggt gaaagtcggc ggatttattc
    11581 gctggctgaa acaggattat gccgacattc cggctcatca agttgaacat tatctcccgc
    11641 agacaccggt tgaagccctg gaaaaaacac cagtgctccc tgcgggaacc gtattcaaag
    11701 gcgacaactg agcgccatca tcctaacgat aaaggtatcc ctatgagcaa tctgagccct
    11761 gactttgtac tacccgaaaa tttttgcgct aacccgcaag aggcgtggac cattcctgcc
    11821 cgtttttata ccgatcagaa cgcgtttgaa cacgaaaaag agaacgtctt cgccaaaagc
    11881 tggatttgcg tcgctcacag cagcgaactg gcgaatgcca atgattatgt gacgcgtgag
    11941 atcattggcg aaagcatcgt gctggtacgc ggtcgtgata aggttttgcg cgcgttctat
    12001 aacgtgtgtc cgcaccgtgg tcatcagttg ttgagcggtg aaggaaaagc aaaaaatgtg
    12061 attacctgcc catatcacgc atgggcattc aaactcgatg gcaatctggc ccatgcacgt
    12121 aactgcgaaa acgtcgccaa tttcgatagc gacaaagcgc aactggttcc ggtgcgtctg
    12181 gaggaatatg ccggattcgt cttcatcaac atggacccca acgccaccag cgtagaagat
    12241 cagttacccg gtctgggcgc gaaagtgctg gaagcctgcc cggaagtcca cgatctgaaa
    12301 ctggcggccc gctttaccac ccgcacgcct gccaactgga agaacattgt cgataactat
    12361 ctcgagtgct atcactgtgg tccggcgcat ccaggtttct ccgactccgt acaggttgat
    12421 cgctactggc acaccatgca cggtaactgg acgctgcaat acggtttcgc caaaccgtcc
    12481 gaacagtcgt ttaaatttga agagggtacg gatgcggcat tccacggttt ctggctgtgg
    12541 ccgtgcacga tgctgaacgt caccccgatc aaagggatga tgacggtcat ttatgaattc
    12601 ccggtggatt ctgaaactac cctgcaaaac tacgatattt acttcaccaa tgaagagtta
    12661 accgacgagc aaaaatcgct gattgagtgg tatcgcgatg tgttccgtcc ggaagattta
    12721 cgtctggttg aaagcgtaca gaaagggctg aaatcgcgtg gctatcgtgg tcaggggcgc
    12781 atcatggccg acagtagcgg tagtggcatt tccgaacatg gtatcgccca tttccataat
    12841 ctgctggcgc aggtgtttaa ggactaatga catcggcggc ggtattttcc gccgctgggc
    12901 tgatttttga tggagtacag caatgtcaga ctatcaaatg tttgaagtac aggtgagtca
    12961 ggtggaaccg cttaccgaac aggtgaaacg cttcacgctg gtggcaaccg atggcaaacc
    13021 gctgcctgcg ttcaccggag gaagtcacat cattgtgcag atgagcgatg gtgataacca
    13081 gtacagcaat gcgtattcac tactgagttc gccgcatgac acctcttgtt atcagattgc
    13141 cgttcggctg gaggaaaact cgcgcggcgg ttcccgcttt ttgcatcagc aggtaaaagt
    13201 gggcgatcgg ttaacgattt caacgcctaa taacctgttt gcgctaattc cctcagccag
    13261 aaagcatctg tttatcgcgg gcggtattgg tatcacccct ttcctgtcgc acatggcaga
    13321 gttgcaacac agcgacgtcg actggcagct acattactgc tcgcgaaatc cagaaagttg
    13381 cgcatttcgt gatgagctag tccagcatcc gcaggctgag aaagtccatt tgcatcattc
    13441 gtcaaccgga acacgactgg aattagcgcg attattggcg gatatcgaac ctggcacaca
    13501 cgtttatacc tgtggccccg aggcgctaaa tgaagcggta agaagtgaag ctgcgcgtct
    13561 ggacatcgcc gccgatacgc tgcactttga gcaatttgct atcgaagaca aaaccggcga
    13621 tgcctttacc ctggtgcttg cccgttccgg aaaagagttt gtggtgccgg aagagatgac
    13681 tattttgcag gttattgaaa ataataaagc cgcgaaagtg gaatgtttat gtcgtgaagg
    13741 ggtatgcgga acctgcgaaa cagcaatact ggaaggtgaa gctgaccatc gggatcagta
    13801 ttttagcgat gaagagcgtg ccagccagca aagtatgttg atctgttgtt cgcgtgcgaa
    13861 gggtaaacgc ctggtgttgg atttgtagtt tgccaaagcc ggatgtggcg ctgagcgcgc
    13921 ccagtccggc ttcggaagat tttactgcgg atattcctgc aataaattgt gtaatgcttc
    13981 cgccatcagc tcaccacgcc agccggaaat cagctccggc aaattgttct gcggtttcag
    14041 tttccagtgc cagttcagca gttggttgat ttgccgacgc gatgccagca attcggcgct
    14101 gatcttatgc gtttcgctca cgtcagtaat cagcgactta atcgctttaa acgctttacg
    14161 ataacccggc atgtccatca ggttaagcat cggctgcggt aaggcctctt ccggcaatgt
    14221 ctgcgctttt tccaccagcg ccagcagcgt tttaccgtga aagcggattt cgctaccgga
    14281 taaacccagg ctgtccagtt cgcctaaact tcccggcata taacgcgcga ccgaccacaa
    14341 atgctcttca cgcacgacaa agttcaccgc cagatcacgc tcacgagcct tgcgcagtcg
    14401 ccagtcggct aacagttgca gacaggccag ttggcgtgtg cgtaattgcc aggcattggt
    14461 gatatcacgc caggcatctt ccggcgcaac gacttcctga cgacgcattt gcatcaggcg
    14521 gcattcatcc agcgccgcag gtagccagcc ggaggcctcc gtttctacca taagcttggc
    14581 ggtgatcggt aacagatacc agacatccgc cgctgcgtat tcacactgac gttcggtcag
    14641 cggtctggcc agccagtcgg tgcgcgattc actcttgtcc agcgtaacgc cggaatactc
    14701 ttccaccatg gaagcgaaac cccatgacat cgggcgtccg cagaaggcag caaggatttg
    14761 cgtgtcaatc aggggttgtg gtaattcgcc aaagacattg aggaacactt ccagatcttc
    14821 actgcctgca tggagaaatt ttgtgatgga cggatcgcgc aggatcgctt tcagcggtga
    14881 ccagtcggtg atcccgagag gatcaattag cgccagatgc tcaccatcga aaagttgaat
    14941 caaccccagc tgcgggtaat aagtgcgcgt acgaacaaat tcagtatcct gggctatcgc
    15001 cggaaagaca cggacggctt cacacaaaga agccagcgca tcgtccgtgg taatcatttg
    15061 gtaattcaaa ttgtattctc tttagtgggc gtcaaaaaaa acgccggatt aaccggcgtc
    15121 tgacgactga cttaacgctc aggctttatt gtccactttg ccgcgcgctt cgtcacgtaa
    15181 ttctcgtcgc aaaatttttc cgacgttaga tttcggtaac tcatcacgaa actccaccag
    15241 cttcggtact ttgtagcccg tgagctgacg gcagcaaaag gtcaccagtg actcttcggt
    15301 aagcgatgga tcttttttca ctacgaagat tttcaccgct tcaccactgg agccggaagg
    15361 tacgccaaca gccgcgactt cctgtacgcc aggatgctgc atgacgacat cttcaatctc
    15421 gttgggatag acgttaaaac cggaaaccag aatcatgtct tttttacgat cgacaatgcg
    15481 caggaaccct tcttcatcca tcaccgcgat gtcgccggtg tgtaaccagc catttttgat
    15541 gatctcatcg gtagcatcgg gacgctgcca gtaacccagc atcacctgcg gtcctttgac
    15601 acaaagctca cccggttgac ccggtggtac ttcattatcg tcgtcatcca ccagtttggc
    15661 ttccgtcgac ggcaccggca aaccgatgct accactatga taatcaatat catatgggtt
    15721 aacgctgacc agcggcgcac actcggtaag accatagcct tccagcagat actgtcctgt
    15781 cagtttcacc caacgctctg ccaccacttg ctgcactggc atcccaccgc ctgcggaaag
    15841 atgcagactg gagaaatcca gctgctggaa ctctttattg ttcagcaacg cattgaacaa
    15901 ggtgttaacg ccagtgatag cggtaaacgg atatttcgct aactctttta ccaaccctgg
    15961 aatatcgcgc gggttagtga taagcaggtt ctgcccaccc agttcgataa acagcaggca
    16021 gttaatggtc agggcaaaaa tgtgatacag cggcagtgcc gttactacca gttctttacc
    16081 tggatgcagc aacggcccat aggtggcgtt gacctgttcc aggttcgcca gcatattgcg
    16141 gtgagtcagc atcgcgcctt tcgccacacc tgtggtgccg ccggtgtatt gcaggaaggc
    16201 taaatcttcc ggcaccagtt cgggtttgac gtactgcatc cggtagccgt tatgcagtgc
    16261 gctacgaaat gaaatggcat ctggcagatg gtatttcggc accaaacgct tgatgtattt
    16321 aacaacgaaa ttgactaccg tgccttttgc cgtagatagc tgatcgccca tacgggtcag
    16381 aattacgtgc tgaacggcgg ttttatcaac cactttttcc agtgtgtgag caaagttaga
    16441 cacgataaca atcgccgatg cgccgctatc gttaagctga tgctcaagct cacgcggggt
    16501 atacaacggg ttaacgttta cgacgatcat cccggcacgc aaaatgccaa acagcgccac
    16561 cggatattgc aataaattag gcatcatcaa cgcaacgcga tcgcctttct tcagccccaa
    16621 cccttgttgc aaataagcgg caaacgcgcg actgcgttct tccagcttgc ggaaggtcat
    16681 tacctccccc atattcacaa acgcaggctg atcggcgtag cggacgaccg actgctcaaa
    16741 catatctatc agagattgat aacggtcagg gttgatctcc gtcggaacat ccgcgggata
    16801 acggttaagc caaaccttct tcaattcttc acctctaaaa tgcgtgttcg tcgtcatcgc
    16861 aaccccaagt gataaacatg ttgttaacat aacattaact catcttacca gcttgataat
    16921 tagacaacga aaaggttgcg aagcgcgtca ctatttattt ttatagttgc cgtatgaacg
    16981 cagaaacagc ggaccagccg ctgtttcttt ttctttaaaa acaacttgtt actcggttac
    17041 aattgtttca actcttgcag ggccaggatt ataccaaccc catccgccgt aaccataggg
    17101 ccagccgcgt ccaccataaa accatggatc aattggctgc ggcggcataa tcacctgttg
    17161 agtgatatgc cagcgtttgt aacctgttac ctgcataacc ataaatttat atggtgcatt
    17221 atttactttg ccatccactg tgccagtgat tggcccgaca accgtcacca gttgtccacg
    17281 aaaatcgacc ggatcaagaa aaccgttcac atcggcataa atgcgcccgc gtgaaggttc
    17341 tcccaaggtt ggtcttgctc cgctgtccag cggtacggta gcaatttcca ggcgggtttt
    17401 cccttgctgg ttttgtacca caaccacttt gccgccaaag cgcgcctcct gaccaacata
    17461 taaatctggc gcattcatca cgcgtaccag atcttgctgt ggtgttggac tgctgccttt
    17521 aatggcgtcc ggcacagtga cacaaccgct cagcattagc gcaaacgtac ctgccagtat
    17581 gcctttgata acattctttt gaaccgccat gatgcgactc ctttttttct cagggcatac
    17641 tcttaagatt cattctttgc ccggaagttt cttccatgcg acgttgttac gtaaataaac
    17701 cggttcggca tgttccaccg ccaccgtttt accctcggca aacatctgac acgcaatcgg
    17761 cagcatatct tccgccgctg gcagtaaaac ttctccgtcg cgcaaaacca gcccgctctc
    17821 tttacggaga tccggccagg cttgccagcc cgtccctacc gttacccatt cgccggaaag
    17881 ctgttgcatt cgttcatgaa cgagttcggg tttgagtacg gcttcggttt cttcaccgcg
    17941 ccagataccg ttttcatcac gctgatattc ggcccagtaa acttcgccca ttcgcgcgtc
    18001 aatggctgcc agcacgcggg ttgcgccgtt tttgcgccac gccccctgcg ccatcgtcat
    18061 tagcgtagag acaccaatca tcggtaactc cgcgccaagc gccagccctt gcgcaatacc
    18121 aataccaatg cgcacgccag taaagctacc ggggccgcgc ccgtaagcca gagcgtttat
    18181 gtcagtcagg gaagttccgc tggtggtcag gatatcctgc accatcggta agattcgttg
    18241 agtatgttca cgagggcaaa gctcaaaatg agcgttgaca ttaccgtcgt tccacagagc
    18301 gacagagcag acctctgtcg cggtatcgat agccagaatt cgcatgggtc ttcgtgctta
    18361 gatcaataaa aaggcgcgca tcataccata ctccgtaaca aattacctgg aggatggtat
    18421 cgcaaggaaa cgaaccgcac gggcaatgtc acgggtgcgt ggcgcgggcg gcagactggc
    18481 gagaaacgtc gcgccgtaag gacgcatcac cagccgattg tcacaaatta ccagcacgcc
    18541 gcgatcgtcg gcgtcgcgaa tcagtcgccc tactccctgc ttgagagtaa tgaccgcatc
    18601 cggcagctgc acttcatcga acgggtcgcc accgcgcaaa cgacaatctt ccatgcgcgc
    18661 ttttaacagt agatcatccg gcgaggtaaa cggcaatttg tcgataatta ccaatgacaa
    18721 tgtatcgcca cgcacgtcca ccccttccca gaaactactg gttgccacaa gaagcgcgtt
    18781 accggcgctg acaaattgct gcaacagttg ccctttgctg gtttccccct gcaacaaaac
    18841 gggaagcgtc atggtagcgc ggaactgctc ggccagatcg cgcatcatgg catgcgaggt
    18901 acaaagcata aaacaacgac cgttgttagc ttcgatgatc ggtcgcagca ttgccgccag
    18961 ttgccgtgca caacccggtt gattggtttg cggcaggttg cgcggcacac agagtaacgc
    19021 ctggcggctg taatcaaacg ggcttggcaa tagcaacgac tccgcctgtt ctatcccaag
    19081 ccgcgaggtg aaatgatgca gatcgtcgtt caccgacagc gttgctgagg tgaagatcca
    19141 gctaccgggc ttttgcgcca ttaactcttt gaatttatcc gccacgctga gcggcgtgag
    19201 agccagagta aaatggcgcg aagtgcattc gtaccagtag ctgtagcccg gctgattgat
    19261 ctcttttagc cgcttcagcc gtgtgcgata caacgtggcg cgctcaaatg ccgcatccag
    19321 caaggcggaa cgccccagtg acagtttcgc cacgtcataa caaagttcca gggtgtcatc
    19381 gagcagtaaa aatgcccgct gaatttgcgg attagctaac agctcacgca ggttaccgcg
    19441 ataacctggc tcaccgagtt gcagacgaaa atcctgtgca ctctgggcaa gacgatcagc
    19501 gcacttttgt aactgctggg tgtcttttaa ttcggtgcgg taggcgatgg tgatgtcttt
    19561 tgccaggtcg agcagttgtc gactggagag tgactgacca aaatactggc tggcaatgtc
    19621 cggtagctgg tgggcttcgt cgaagatcat gacgtccgct tcagggatca actcgccaaa
    19681 tccactctct ttaaccacca tatccgccag aaagagatga tggtttacca ccaccacatc
    19741 ggcgtccatc gcttttttac gtgctttgac cacaaagcaa tctttataca tcgggcagtc
    19801 gctgccaaga cagttgtcgt tggtgctggt gaccagcggc cacgcctgtg aatcttccgc
    19861 cacgctgacg caggtgctga tatcaccatc gactgtttga ttagaccagg agcgcagcag
    19921 gatcacatcg cttaagattt gtaccggcag atcgcccccc gccagcgcct gctgttcgag
    19981 acgttcgagg cagaggtagt ttgagcgccc tttcagcagc gccacgttgc ccgtatattt
    20041 caatgccttt gagactgttg gcaaatcgcg gctgtagagc tgatcctgca acgcttttga
    20101 gccggtcgag ataatgactt tctttttcgc ccgcagcgca ggagccaggt aagcgtaggt
    20161 tttgcccgta ccggttcctg cttccaccac cagcggctgg cctttttcta tcgcctgggt
    20221 gacggctacc gccatctgtc gctgtggttc tcgcggctta aagcctggta tcgctttcgc
    20281 cagctgaccg tctggtgcaa aatcgtccgt cacactaccc cctgttgatt tgaacaggga
    20341 ttatgtcagg atgagggcgc tttcgccagt tgaagtggtg acggcaacct cacattgtgg
    20401 cagtctttgc agcacgaaaa tggaagtata atgaggacaa aatgatgact atcgttcgta
    20461 tcgatgctga agcccgctgg tctgatgtag taatccacaa caacacgctc tactacactg
    20521 gtgtaccgga aaacctcgac gccgatgcct ttgagcaaac cgccaacacg ctggcacaga
    20581 ttgacgccgt gctggaaaaa cagggcagca ataaatcgag cattctggat gccaccattt
    20641 tcctggccga taaaaacgac ttcgcggcga tgaataaagc gtgggatgcc tgggttgtcg
    20701 cgggtcatgc gccagtgcgc tgcacggtac aagcgggttt gatgaacccg aagtataaag
    20761 ttgaaattaa gattgtggct gcggtgtaag ctttatcgaa gcaaaataag tcagacgata
    20821 atttatcgat aatactggtc ggttttacat aaatcgacca gagaataaga tattactgtt
//
LOCUS       DAEEKQ010000061        20759 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.061, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000061 DAEEKQ010000000
VERSION     DAEEKQ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20759
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.061"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(131..1780)
                     /gene="actP"
                     /locus_tag="KLK57_002160"
     CDS             complement(131..1780)
                     /gene="actP"
                     /locus_tag="KLK57_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HBE0892524.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKLVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSITVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1777..2090)
                     /locus_tag="KLK57_002161"
                     /pseudo
     CDS             complement(1777..2090)
                     /locus_tag="KLK57_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
     gene            complement(2222..4180)
                     /gene="acs"
                     /locus_tag="KLK57_002162"
     CDS             complement(2222..4180)
                     /gene="acs"
                     /locus_tag="KLK57_002162"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HBE0892525.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEKMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4574..6010
                     /gene="nrfA"
                     /locus_tag="KLK57_002163"
     CDS             4574..6010
                     /gene="nrfA"
                     /locus_tag="KLK57_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HBE0892526.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6055..6621
                     /gene="nrfB"
                     /locus_tag="KLK57_002164"
     CDS             6055..6621
                     /gene="nrfB"
                     /locus_tag="KLK57_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HBE0892527.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6618..7289
                     /gene="nrfC"
                     /locus_tag="KLK57_002165"
     CDS             6618..7289
                     /gene="nrfC"
                     /locus_tag="KLK57_002165"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HBE0892528.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7286..8242
                     /gene="nrfD"
                     /locus_tag="KLK57_002166"
     CDS             7286..8242
                     /gene="nrfD"
                     /locus_tag="KLK57_002166"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HBE0892529.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8262..9980
                     /locus_tag="KLK57_002167"
     CDS             8262..9980
                     /locus_tag="KLK57_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HBE0892530.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWEFKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFCH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRAQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            9973..10356
                     /gene="nrfF"
                     /locus_tag="KLK57_002168"
     CDS             9973..10356
                     /gene="nrfF"
                     /locus_tag="KLK57_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HBE0892531.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10353..10949
                     /gene="nrfG"
                     /locus_tag="KLK57_002169"
     CDS             10353..10949
                     /gene="nrfG"
                     /locus_tag="KLK57_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HBE0892532.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTCAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11291..12604
                     /gene="gltP"
                     /locus_tag="KLK57_002170"
     CDS             11291..12604
                     /gene="gltP"
                     /locus_tag="KLK57_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HBE0892533.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12683..13372)
                     /locus_tag="KLK57_002171"
     CDS             complement(12683..13372)
                     /locus_tag="KLK57_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HBE0892534.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13466..15613)
                     /gene="fdhF"
                     /locus_tag="KLK57_002172"
     CDS             complement(13466..15613)
                     /gene="fdhF"
                     /locus_tag="KLK57_002172"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15194..15196),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HBE0892535.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVTERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15811..17277)
                     /gene="mdtP"
                     /locus_tag="KLK57_002173"
     CDS             complement(15811..17277)
                     /gene="mdtP"
                     /locus_tag="KLK57_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HBE0892536.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17274..19325)
                     /gene="mdtO"
                     /locus_tag="KLK57_002174"
     CDS             complement(17274..19325)
                     /gene="mdtO"
                     /locus_tag="KLK57_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HBE0892537.1"
                     /translation="MSALNSLPLPMVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGRMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAISQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRISESEAVAARECNLENICQTLLQLGQMNPNTPPTPA
                     AKPPSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVSNPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19325..20356)
                     /gene="mdtN"
                     /locus_tag="KLK57_002175"
     CDS             complement(19325..20356)
                     /gene="mdtN"
                     /locus_tag="KLK57_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HBE0892538.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSINATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20375..20650)
                     /locus_tag="KLK57_002176"
     CDS             complement(20375..20650)
                     /locus_tag="KLK57_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892539.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4676 a         5497 c         5684 g         4902 t
ORIGIN      
        1 tatcattttc tacgactgtc aaaaatcgct cattttttaa tgagtttatt tgtttaatat
       61 tttgggaaaa ggtgatgcat gctggagagg aagagtattc cccggtcagg cgaccgggga
      121 aggggagaga ttaatgtgcg cggccttgct caacgccaaa gccggtctgg gagcggataa
      181 actgcgcgcg gaacagttca cgctcgcgcg ccccttccgc tgagttatcg gttgccgaga
      241 agaaccagat gccgaggaat gccacggtga tagagaacag cgccgggtat tcatacggga
      301 agatggcttt ttcgtgacca aggatctgta cccaaatcgt cgggccgagg atcatcagta
      361 ctactgcggt aatcagcccc agccagccac ccatcatcgc gccacgcgtg gtcagtttcg
      421 accagtacat agaaagcaga atgatcggga agttacagct cgccgcgatg gcaaacgcca
      481 gccccaccat aaaggcgatg ttctgattct caaacagcac gccgaggata atcgcaatca
      541 cgccgaggat cagtacggtg atttttgata cccgcagctc ttcacgttcg gtcgcgcctt
      601 ttttgaagac gttagcgtac aagtcatgcg aaaccgcgga tgcgcccgcc agcgtcagac
      661 ccgcaaccac cgcgaggata gtggcgaaag caaccgctga aataaaaccg aggaacaggt
      721 tgccgcccac tgcattcgcc aggtgaacgg ccgccatgtt gttaccacca atcagatggc
      781 ccgccgcgtc tttatattcc ggattcgcac caaccagcat gatcgcgccg aagccgataa
      841 taaaggtcag aatatagaag tagcccataa acccggtggc gtagaacacg ctcttacgtg
      901 cttcgcgggc atcgctgact gtaaagaagc gcatcagaat gtgcggcaag cccgccgtac
      961 caaacatcag tcccagaccc agagagagtg cggagatcgg atctttcacc agcccgcccg
     1021 gcttcatgat gtcgacacct ttcgggtgta ccgccatcgc ttcactgaac agattgttga
     1081 agctaaagcc gacgtgtttc atcaccataa aggccataaa gctggcaccg aacagcaaca
     1141 gcacggcttt gataatctgt acccaagtgg tcgccagcat gccgccgaac aggacgtaca
     1201 tcatcatcag cacgccgacc agcaccaccg caatgtgata gttaaggcca aacagcagct
     1261 cgatcagttt acctgcgccc accatctggg cgataaggta aagcgccacc accaccagag
     1321 aaccacaggc cgaaagaata cgaatcggcc cttgtttcag acggtaagag gccacatcgg
     1381 caaaggtgta gcgccccagg ttacgcagac gttcggcaat caggaacaaa atgatcggcc
     1441 agcccaccag gaagcccagc gagtaaatta agccgtcata gccggaggta aacaccagcg
     1501 cggagatccc caagaatgag gcggcggaca tatagtcccc ggcaatcgcc agcccgttct
     1561 ggaagccagt gatattgccg cctgcggtgt agtagtcgct acgagaacgt acgagttttg
     1621 atgcccagta ggtaatgccg agcgtaaaca cgacgaaaat caggaacata ataatcgcct
     1681 gccagttcgt tggctggcgc tctacggccc cgctaatagc atccgcggcg ttagctgcga
     1741 aagggagtgt ggcggcaagc gccgtcagaa ctcttttcat gatgcttgta cctcatgcag
     1801 gacttcatta ttaagacggt cgaattcgcc gttcgcccgc cagatgtaga taccggtgag
     1861 aacaaaggag atcacaatca ctccaacacc aattggaata ccgcgtgtga cgctggtgtt
     1921 cggattcagc ggcgtgccag ccagccgggc gcgaaggcga tcagtaaaat aaagccgata
     1981 taaactgcca gcataataat cgacaggatg gtggcaaacc gttgccgttt ttcgactaac
     2041 tccctgaaat gcgcattgtc ttctatccgc tgataaatag tgccattcat cacagattct
     2101 ccagaggtaa tgtagggatt gttttaattc ccgctccctt atgggagaag gttaacgctc
     2161 gggtaaccct tgccgaatgt aggccggata aggcgtttac gccgcatccg gcaattgtgg
     2221 gttacgatgg catcgcgata gcctgcttct cttcaagcag cttctcgact acgccaggat
     2281 cggcaagcgt cgaggtatcg cccaggttgc tggtatcgcc cgccgcaatt ttgcgcagaa
     2341 tacggcgcat aattttgccg gagcgggttt taggcaggga gtcggtccag tgcagcacgt
     2401 ctggcgtcgc cagcgggcca atctctttac gcacccagtt gcggacttct gcgtacagtt
     2461 ctggtgacgg ttcctccccg tgattaagcg tgacgtaggc gtagatcgcc tgacctttaa
     2521 tattgtgcgg aatacctact acggcggctt cggcaatctt cggatgcgcc accagcgccg
     2581 actcaatctc tgccgtcccc agacggtgac cggagacgtt cagcacgtcg tccacacgcc
     2641 cggtgatcca gtaatagcca tcttcatcgc gacgcgcgcc gtcgccgctg aaatacatat
     2701 ttttgaaggt ggagaagtag gtctgttcaa aacgttcgtg atcgccaaac agcgtacgcg
     2761 cctgacccgg ccaggagtcg gtgatcacca ggctgccttc ggtagcgcct tccagcgggt
     2821 taccttcgtt atcgaccagc gccggttgca cgccgaagaa cggacgtgtt gccgaaccgg
     2881 ctttcagctc ggtagcgcca ggcagcggcg tgatcatgaa accgccagtt tcggtctgcc
     2941 accaggtatc gaccaccgga catttctcgt tgccgatttt tttccagtac cactcccacg
     3001 cttccgggtt aattggctcg cccacggaac cgagaatgcg cagcgacgaa cggtcggtac
     3061 cctcgatcgc tttatcgcct tccgccatca gcgcgcggat cgccgtgggt gcggtataga
     3121 gaatattgac ctgatgcttg tccaccacct gcgccatacg ggcaggcgtc ggccagttgg
     3181 gtacgccttc aaacatcagc gtggtcgcac cgcaggccag cgggccgtac agcaagtaac
     3241 tgtgtccggt cacccagccc acatcggcgg tgcaccagta gatatcaccc ggatgataat
     3301 caaagacata tttaaaggtc agcgccgcgt acaccagata accgccggta gtatgcagca
     3361 caccttttgg cttaccggta gaaccggagg tgtagagaat aaacagcgga tcttcggcgt
     3421 tcatcttttc cgcctggtgc tgatcgctcg cttgctcaac caggtcgtgc caccacaggt
     3481 cgcgcccttc ctgccagtca attttcccgc cagtacgctt cagtaccacc acatgctcta
     3541 cgctggtgac gttcgggttt ttcagcgcgt catcaacgtt tttcttcagc ggaatactgc
     3601 gcccggcacg cacaccttcg tcggaagtga tcaccagtcg tgagttggaa tcaataatgc
     3661 gcccggcaac ggcttccggc gagaagccgc cgaaaatcac cgaatgcacc gcgccaatgc
     3721 gggcgcaggc cagcatcgca accgcggctt ccggcaccat cggcatataa atcgccacca
     3781 catcaccttt tttaatgccc agctcgagca gggtattggc gaagcggcag acgtcgcggt
     3841 gcagctcttt atagctgata tgtttgctct ggctggcgtc gtcgccttcc cagatgatgg
     3901 cggtacgatc gccgttttct tgcagatggc ggtcaaggca gtttgccgcc agattcagcg
     3961 tgccgtcctc gtaccattta atggacacat taccgggggc aaaggaggtg tttttcacct
     4021 tctggtaagg tttgatccag tcaagaattt ttccctgttc gccccagaag gtatcaggta
     4081 cgttaataga ttgttgatac atcgcctcgt actgctgagg gtttatcagg caacggtctg
     4141 cgatgttggc aggaatggtg tgtttgtgaa tttggctcat gcttttgttc tccttgtagg
     4201 gtgttaataa tatgtggcat aagcgttaaa tgtaggggta ttggcagttt tgtttagtat
     4261 ttgggcgaca gatcacgcaa aagtagaatt gtgcaaataa acggcagggt aatttttgaa
     4321 ggtcagcaac aaaagttgat taattctttg aggaacatgc agttatgcat tgctgttgaa
     4381 aaagaggaag atactaacta actctaaagt ggtattttac atgcacttac aattgattaa
     4441 agacaacatt cacagtgtgg ttatttgtta cacatagggg cgagcaatgt catgacagtg
     4501 taggtgcggt tactgtcgtg aaaaacaata aaaaccgcca ttgtaacaat ggcgcaattc
     4561 ggatgaagcc cctatgacaa ggataaaaat aaacgcacgc cgtatcttca gcttattgat
     4621 tccttttttc tttttcactt ctgttcacgc tgaacaaacg gcagctcccg caaaacctgt
     4681 aactgtggaa gcgaagaatg aaacctttgc cccgcagcat cccgatcaat atctctcctg
     4741 gaaagccacc tcggaacagt cagagcgtgt tgacgccctg gcggaagatc cacggctggt
     4801 gatcctgtgg gcggggtatc ccttctcgcg cgattacaac aagccgcgtg gacatgcctt
     4861 tgctgtgacc gatgtgcgtg aaaccctgcg taccggtgcg ccgaaaaacg ctgaagatgg
     4921 tccgctaccg atggcgtgct ggagttgtaa aagcccggat gtggcgcgtc tgatccagaa
     4981 agacggcgaa gatggctact tccacggtaa gtgggcgcgc ggcggcccgg aaatcgtcaa
     5041 caacttaggt tgtgccgact gccataacac cgcctcacca gagttcgcca aaggcaaacc
     5101 ggagttaacc ctttcccgtc cgtatgcggc tcgcgcgatg gaagccattg gtaaaccttt
     5161 tgagaaagcc ggacgtttcg accagcaatc gatggtttgc ggtcagtgcc atgtggagta
     5221 ttacttcgac ggcaaaaaca aagcggttaa attcccgtgg gatgacggca tgaaagtcga
     5281 aaatatggag cagtattacg acaaaattgc cttctctgac tggactaact ccctgtcgaa
     5341 aacgccaatg ctgaaagcgc agcacccgga atatgaaacc tggacagcgg gcattcacgg
     5401 taaaaacaac gtgacctgta tcgactgcca tatgccaaaa gtgcagaacg ccgaaggcaa
     5461 actctatacc gaccataaaa ttggtaatcc gtttgataac ttcgcccaga cttgtgcgaa
     5521 ctgccatacc caggacaaag ctgccttgca aaaagtggtc gcggaacgta agcagtcgat
     5581 taacgacctg aaaatcaagg ttgaagatca actggttcac gctcacttcg aagcgaaagc
     5641 agcgctggat gcaggcgcga cggaagctga aatgaagcca attcaggacg atatccgtca
     5701 tgcccagtgg cgctgggatc tggcgatcgc ttcccacggc attcatatgc acgcaccgga
     5761 agaaggttta cggatgctcg gtacggcgat ggataaagcg gcggatgcac gcaccaaact
     5821 ggcgcgcctg ctggcgacca aaggcatcac ccatgaaatc cagatcccgg atatctcaac
     5881 caaagagaaa gcccagcagg ccattggcct gaacatggaa caaatcaagg ccgagaagca
     5941 ggacttcatc aaaacggtga tcccgcagtg ggaagaacag gcacgtaaaa acggtctgtt
     6001 aagccaataa ccccgttccg cctcgcaagg ggcggaaaac acaatggagt gaatatgagc
     6061 gtattacgtt cgttattaac tgccggggtg ctggcgtcgg gcctgttgtg gagcctgaac
     6121 ggaattaccg ctacccctgc ggcgcaggca tctgatgatc gctacgaagt tacccagcag
     6181 cgtaacccgg atgccgcctg tctggactgt cataaaccag ataccgaagg tatgcatggc
     6241 aaacatgctt ccgttatcaa cccgaataac aaactgccgg tcacctgcac caactgtcac
     6301 ggccagccat caccgcaaca ccgcgaaggg gtgaaagatg tgatgcgctt taacgagccg
     6361 atgtacaagg ttggagagca gaacagcgtc tgtatgtcct gtcatctgcc ggaacagttg
     6421 caaaaagcgt tctggccgca cgatgtccac gtaaccaaag tggcgtgcgc cagctgccat
     6481 tccctgcatc cacagcaaga tacgatgcag acgttaagtg acaaaggacg gattaagatt
     6541 tgcgtcgatt gccacagcga tcagcgcacc aatccgaact ttaacccggc gtctgttccg
     6601 ttgcttaagg agcagccatg acctggtctc gtcgccagtt tctcaccggc gtcggcgtgc
     6661 tggcagccgt cagcggcacc gcagggcgcg tggtggcgaa aacgttgaat atcaatgggg
     6721 tgcgttacgg catggtgcat gacgagtcgt tatgcatcgg ctgtaccgcc tgtatggatg
     6781 cttgtcggga agtgaacaag gtgccggaag gcgtctcgcg cctgacgatt attcgtagcg
     6841 agccgcaggg cgaatttcct gatgtgaaat atcgcttctt ccgtaagtct tgccagcact
     6901 gcgatcatgc gccgtgcgtt gacgtctgcc cgaccggtgc gtcttttcgt gatgccgcta
     6961 gcgggattgt cgatgttaat cctgatctct gcgtcggttg tcagtactgc atcgccgcct
     7021 gtccgtaccg cgtgcgcttt atccatccgg tcacgaagac ggcggacaaa tgcgatttct
     7081 gccgtaaaac caatttgcag gccggtaagc tgcccgcgtg tgttgaggct tgcccgacga
     7141 aggcgctgac gtttggcaat ctggacgatc ctaacagtga gatttcgcaa ctgctgcgcc
     7201 agaagcctac ttaccgctac aagctggcgc tgggaaccaa accgaagctg taccgcgtac
     7261 cgtttaaata cggggaggtg agccaatgac gcagacttcc gcatttcatt ttgaatcgct
     7321 ggtgtgggac tggccgattg ccatctacct gtttttgatt ggtatttctg ccggtctggt
     7381 gacgctggcc gtgctgttac gtcgcttcta cccgcaggcg ggcggtgcag acagtacgtt
     7441 gctgcgcacc acgctgattg tcgggccggg cgcggtgatc ctcggtctgt tgatcctcgt
     7501 cttccacctg acaagaccgt ggaccttctg gaagctgatg ttccactaca gttttacctc
     7561 ggtgatgtcg atgggggtga tgctgtttca gctctacatg gtggtgctgg tgctgtggct
     7621 ggcgaaaatc tttgaacatg atttgcttgc cctgcaacaa cgctggttgc cgaagctggg
     7681 gatcgtgcaa aaggttctga gcctgctgac gcccgttcat cgcggactgg aaacgctgat
     7741 gctggtgttg gcggtgctgc tgggggctta taccggcttt ctgctgtcgg cgctgaaatc
     7801 gtatccgttc ctcaataacc cgatcctgcc ggtgctgttc ctcttctccg gcatctcgtc
     7861 cggtgcggcg gtggcgctga tcgccatggc gatacgccaa cgcagtaacc cgcattccac
     7921 ggaagcgcac tttgttcacc ggatggaaat ccccgtggta tggggtgaaa tcttcctgct
     7981 ggtggcgttt tttgtcggtc tggcgctggg cgatgacggt aaagttcgtg cgctggtggc
     8041 ggcattaggt ggcggtttct ggacgtggtg gttctggctt ggtgtcgccg ggctggggct
     8101 gattgtgcca atgttgctca aaccgtgggt caatcgcagt tccggcattc ctgccgtgct
     8161 ggcggcgtgt ggggccagtc tggtcggcgt gttgatgctg cgctttttca ttctctacgc
     8221 cggacagttg acggtggcgt aagccagaaa agaggtggtt tctggacgta ttccttcctg
     8281 aagtcggttt tctggcgttg ttgttaagtc tcggggtcaa cgtgttgacc ccgttgacgg
     8341 cctttgcggg agtgcggttg cgctggcctg ccatgatgcg actcacttgc atcggcattc
     8401 tggcgcagtt cgcgctcctg ctgctcgcct ttggcgtact gacgtattgt tttctcatca
     8461 gcgatttctc ggtcatttat gtcgcgcaac atagctatag cctgctgtcg tgggaattca
     8521 agctggcagc ggtgtggggc ggccatgaag gttcgctgct gctttgggtg ctgctgcttt
     8581 ccgcctggag cgcgctgttt gcctggcatt atcggcagca aaccgatccg ctatttccgc
     8641 tgacgttagc cgttttatct ctcatgctcg ccgcactgct actgtttgtg gtgctgtggt
     8701 ccgatccctt cgtgcggata tttccaccag caatcgaagg ccgcgatctc aatccgatgc
     8761 tgcaacatcc cggtcttatc tttcatccac cgctgcttta ccttggctat ggcggtttga
     8821 tggtagcggc gagcgtggcg ctggcgagtc tactgcgcgg cgagtttgat gcggccagcg
     8881 cccgaatttg ctggcgctgg gcgttacctg gctggagtgc attaacggcg gggatcatcc
     8941 tcggttcctg gtgggcctac tgcgaactcg gctggggcgg ctggtggttc tgggatccgg
     9001 tggaaaatgc ctctttatta ccctggcttt ctgccactgc gctgctgcac agtttatccc
     9061 tgacacgcca gcgggggatt ttttgccact ggtcgctgtt gctggcgata gttactctga
     9121 tgctgtcgct gctgggcacc ttaattgtcc gttctggcat tctggtttcg gttcatgcgt
     9181 tcgcactgga taacgtccgc gccgtgccgt tgttcagcct gtttgcactg attagccttg
     9241 cgtctttggc tctgtatggc tggcgagcgc gggacggtgg cccggcggtg cgtttttcgg
     9301 ggttatcgcg ggaaatgtta atcctcgcta cgctgttgct gttttgcgca gtgctactga
     9361 tcgtgctggt gggaacgctt tatccgatga tttacggtct gctaggctgg ggacgcctct
     9421 ccgttggcgc gccgtatttt aaccgcgcga cgttaccgtt tggtctgttg atgctggtgg
     9481 tgattgtgct ggcgacgttt gtctctggca aacgcgcgca gcttccggcg ctggtcgctc
     9541 atgcgggcgt gctgttattt gccgcaggga tcgtggtttc cagcgtcagc cgtcaggaga
     9601 tcagcctgaa tttacagccg ggtcagcagg tgacgctggc aggatacacc ttccgttttg
     9661 agcgcctcga tctgcaagcc aaaggcaatt acaccagcga aaaagcgata gtggcgctgt
     9721 ttgaccatca gcaacgcatt ggtgaattaa cgccggagcg gcgtttttat gaagcacgcc
     9781 gtcagcaaat gatggaaccg tcaattcgct ggaacggcat ccatgactgg tatgcggtca
     9841 tgggtgaaaa aaccggagcg gatcgttacg cttttcgttt gtatgtacaa agcggtgtgc
     9901 gctggatctg gggtggagga ttgttgatga ttgcgggcgc attgttaagc ggatggcggg
     9961 ggaagaagcg cgatgaataa agggcttctc acgctactgc tgttatttac ctgttttgcg
    10021 cgcgctcagg tcgtagacac ctggcaattc gccaatccgc aacaacagca acaggcgtta
    10081 aatattgcca gccagttacg ttgtccgcag tgccagaatc aaaacttact cgaatccaat
    10141 gcgccggtgg cggtcagtat gcgccatcag gtttatagca tggtgtcgga ggggaagaac
    10201 gaagtcgaaa tcattggctg gatgaccgaa cgctacggag attttgttcg ctataaccca
    10261 ccgttaacgg gtcagacgct ggtgttgtgg gcgctgccag tggtgttgtt attgctgatg
    10321 gcactgatcc tctggcgagt gagggctaag cgatgaaaca gcctcaaata ccggtgaaaa
    10381 tgctgacaac actcacgata ttgatggtat tcctctgtgt cggcagttat ctgttaagcc
    10441 caaaatggca ggcggtacgt gcggagtatc agcgtcagcg cgatccgcta catcagtttg
    10501 ccagccagca aaccccggaa gcgcagcttc aggcattgca ggataaaatc cgcgctaatc
    10561 cacaaaacag cgaacaatgg gcgttactgg gcgagtacta tctgtggcaa aacgattaca
    10621 gcaattcgct gctggcgtat cgtcaggcgt tgcaactacg tggtgagaac gctgaactgt
    10681 atgcggcgct ggcgacggtg ctttattacc aggccagcca gcatatgacc gcccagactt
    10741 gcgcaatgat cgacaaagcc ctcgcgctgg actctaacga aatcaccgct ctgatgctgc
    10801 tggcttccga tgcgtttatg caggcgaatt acgcgcaagc catcgaacta tggcaaaaag
    10861 tgatggatct caactcaccc cggatcaacc ggacacagct ggtcgagtcg attaatatgg
    10921 caaaactgtt gcagcggaga tcggattaat catctggtga tgaagattat gtcattgagc
    10981 tgcataaaaa ataatcgaat gaacatatgc caaaaataat cactaatcag tattattgca
    11041 gattaaacaa ataaaaatct ttccataaca aatggttatt cattaatcct gccataaact
    11101 cgcgttatta tgcattaatg cagcgaaaag ctctgttgtt aaagggttgc gcaacatacc
    11161 gcgcaaatga tactgatcat aagcgttaaa aaaatctaca aaccaacgca acacaattca
    11221 tgccctggca gtatgtcacg ttctcgcgtt tctgaacggg gaacggcgct ccattgagga
    11281 agttattctg atgaaaaata taaaattcag cctggcctgg cagattctgt ttgctatggt
    11341 gctgggcatt ctcctgggaa gctacctgca ctaccatagc gacagccgcg actggctggt
    11401 cgtcaatttg ctctctccgg cgggtgatat cttcatccat ctgatcaaaa tgattgttgt
    11461 gccgattgtg atctccacgc tggtggtggg tatcgcgggt gttggtgatg ccaaacagct
    11521 cgggcgtatt ggcgcgaaaa ccattattta cttcgaggtg atcaccaccg tcgccatcat
    11581 tttggggatc actctggcga acgtcttcca gcccggtgcc ggggtggata tgtcgcagct
    11641 ggcgaccgtc gatatctcga aatatcagag cactacggaa gcggtacaaa gcagttccca
    11701 cggcattatg ggcacgattt tgtcgctggt gccgacgaac attgtggcgt cgatggcgaa
    11761 aggcgaaatg ctgccgatca tctttttctc ggtgctgttt ggtctggggc tttcttccct
    11821 gcccgcgacg catcgtgaac cgctggtgac cgtgttccgc tccatctctg aaaccatgtt
    11881 taaagtgact cacatggtga tgcgttatgc accggtgggt gtgtttgcgc tgattgcggt
    11941 gacggtggct aactttggtt tctcttctct gtggccactg gcgaaactgg tgctgctggt
    12001 gcatttcgcc attctgttct tcgcgctggt agtgctggga attgtggcgc gcctgtgcgg
    12061 gttaagcgtc tggatcctga ttcgtattct gaaagatgag ctgattctgg cgtactccac
    12121 tgccagctct gaaagcgtac tgccgcgaat tattgagaag atggaagcct acggagcacc
    12181 ggcgtcgatc accagcttcg tggtgccgac cggttactct tttaaccttg atggttcgac
    12241 gctgtatcaa agtattgccg ctattttcat cgctcagctg tatggcattg acctgtccat
    12301 ctggcaggaa atcattctgg tgctgacgct gatggtgacc tcgaaaggga ttgctggcgt
    12361 gccgggcgtg tcgtttgtgg tgctgctggc aacgctgggt agcgtaggta ttccgctgga
    12421 aggtctggcg tttattgctg gtgttgaccg tatcctcgac atggcgcgta ctgcgctgaa
    12481 cgtggtgggt aatgcgctgg cggtgctggt gattgccaag tgggaacaca aatttgaccg
    12541 caaaaaggcg ctggcttatg agcgtgaagt gctgggcaaa tttgataaaa ctgcggatca
    12601 ataattgaag attgccgggg atatccaccc ggcaatgtgt gaatgcctga tgcgacgctt
    12661 gccgcgtctt atcaggccta ccttaaccgt tggttaattt ttcaaactct tcacacccgg
    12721 tatcaaaccc ttccatacag ctcaggttca accagtgcag cgccttttgc ttgttcttct
    12781 cgataaaacc ttcttcaccg tttaagaaca tcattcccgc ccagtactcg gaataaccgg
    12841 ttcgggaaat tgcagagctg cgtttgaaat accaggttgc cttgtcatcg tcggccttaa
    12901 tgcccacgcc gttggcgtaa atcaaaccaa gcagcatttg ggcatcgacg gcagagtcgt
    12961 tctccagatc ttccgaggcg ttttctaaca gcgaaatagc ctttggataa tccggtttac
    13021 ccgcttgagt atttaccaga atatgcgcca gcgttacttc accttcttta ctgcccgctt
    13081 gcgccgcttt ctctgcaaga acttttgctt gtggatagtc cagactgacc ggattggtga
    13141 ttttgatctg cgccagcagc gcgcaggcat cagcatcacc gctgtcggcg gctttctgtg
    13201 cccaatattc ggctttgctc aaatcgccgg agctaaacca gctgtcggca agaaaatatt
    13261 gtgcgcgtcg gtccccggcc tctgctgcct ttaaatactg gctgccaggc tcggagtcgt
    13321 tggcgtgggc aaagaatgtc aaaaacaaca tcaatgcgat aatttttttc attttgataa
    13381 tacgattggg tagggagtaa ccagtataaa gagttcgaat ggataaaaaa acagcctccg
    13441 aaaggaggct gtagaaagga ctgtattacg ccagtgccgc ttcgcgcagg cgagttttca
    13501 acttgttgta ctcgtcaatc acgtactgct cggcggcgcg ctgatcggcg atcggctcga
    13561 cgcgcacggc gcagtatttg tattccggcg ttttcgtaat cgggcttaag ttttcggtaa
    13621 ccagctcgtt acaggcacca atccaccact ggtaggtcat gtaaatcgcc cctttgttcg
    13681 gacgatcgct gacctgcgca cgggtgataa ttttgccttt acgcgagtgc acccaaacca
    13741 atgcctcatc ttcaataccc agacgtttgg cgtcttcggt attgatttgt gcgtagccag
    13801 gttcatcagc cagcgccgcc agtgccgcac agttaccggt catcgaacgg caagagtagt
    13861 gaccaacttc acgcaccgtt gacagtacca tcgggtactc gtcggtgagt ttgtcgattg
    13921 gcgctaccca gtcgcaggtg aagaactgcg ccagaccgtt cggggtatca aacttctctt
    13981 taaacagata agaagtcccc tgatcggcat ctgaagtatc gcggcaaggc cactgaatga
    14041 agcccagttc gcccattttc tcgtaagtcg caccgtagaa atccgggcac agatgacgca
    14101 actcatccca gatctcctgg gtgttgttgt agtgcatcgg ataacccata cgggtggcga
    14161 tttcactgat gatttgccag tccgttttca gatcccattt cggttcaact gctttgaaga
    14221 aacgctggaa gccacggtca gccgcagtaa acacgccttc atgctcgccc cacgacgttg
    14281 acggcaaaat gacatccgcc gccgacgcgg ttttggtcat aaagatgtcc tgaacgataa
    14341 ccagttccag atcttcaaag gctttacgta ctgccgacag ctccgcgtca gtttgcagcg
    14401 gatcttcgcc cataatgtac gcggcacgca cttcgccatg cgctgcgcgg tgcggcagct
    14461 cgctgatgcg ataaccggta tgagcaggca ggctttccac gccccaggct ttggcgaatt
    14521 tctcgcggtt agccggatct ttcacgtact ggtatcccgg atacgtatcc ggcagcgcgc
    14581 ccatatcgca ggcaccctgc acgttgttct gaccacgaac cgggtttaca cccgcatgcg
    14641 gcttaccgag gttaccagtc agcatcgcga ggctggtcag agaacgcacg gtttccacgc
    14701 cctggtagaa ctgggttaca cccatgcccc acaggatggc ggcgcttttc gcctgggcat
    14761 acatccgtgc cgcctgacga atctcactgg cgctgacgcc ggtgatatct tcaaccgact
    14821 ccggcgtgta gccttcaacg attttacgat actcttcaaa gccttctgta cgggaagcga
    14881 cgaacgcttt gtcgtacaga ttttcttcaa taatgacatg gcccatcgca ttcaacagcg
    14941 cgatgttcga gccgtttttc agtgcaatgt gcatgtcagc aatgcgcgcg gtttcaattt
    15001 tgcgcggatc gcagacgata attttcgccc cgttacgttt agcgttaatt acgtgattcg
    15061 ccacgattgg gtgggaatca gccgggttgt acccgaaaac gaacactaaa tcggtattat
    15121 caatttcgtt aatagcattg ctcattgcgc cattaccgac cgattggtgc agacctgcaa
    15181 ccgatgggcc gtgtcagaca cgagcgcagc agtcaacgtt attggtacca ataacggcgc
    15241 gcgcaaattt ttgcattaca tagttggttt cgttacccgt accacgcgag gagccggtcg
    15301 tctggatggc atccggaccg tacttctctt tgatggcgct caggcgctcg gtaacgtaat
    15361 tcagtgcctc atcccaggaa acaggttcga gtttgccgcc acgctggcga cggatcatgg
    15421 gggttttcag gcgcggggtc aggatctggg tatcgttaat gaaatcccag ccataataac
    15481 ccttcagaca cagggtaccc tggttggttt tcccctgcgc tgcctccgcc cggacgattt
    15541 tgccgttatc gacgaccagg ttgattttgc aacctgatgc gcaatagggg caaaccgtga
    15601 cgactttttt catcggtctc gctccagtta atcaaatcac gcatacgcgc tctcgactac
    15661 agtatgcatc ttttatgcca cattttattt ggggtcattc cctgatatta cgggcactat
    15721 ttattcaaaa ctctgacgaa aaacaggctg tcgtcagttt tgacgtgacg aaacgaaata
    15781 ccgcgtgaca gccatcacgc ggcagacatt ttattttttc tcgacgacgg gagccgcctg
    15841 atacccgccg cccagcgatt tcatcaactg aatgctttgg atcacccggc ggctgtccag
    15901 catcagtaat gacatctctt cggcaagtac tggcaaccgg gcttcagtgg cctgtaagcg
    15961 gctggttaag ccgcgctgat aggcggcctc ggcagcgcgc tgggtgaagc gcgttgcttc
    16021 cacgcgttca gcctgcattt ctcgctcgtc gttgagcgtt tgcagacgcg tgccgttgac
    16081 ggcaacgtca cgcaccgcgt tcagtactga ctggttgtaa cgttcaatca tcatgttgct
    16141 ggcggcgcgc gtgccttcga gattggcatt caaccgtcca ccgtcaaaca gcggcaattt
    16201 cagacccggg atgaagttga actggcgact ggtttttttg aataaggtat ccaggtggat
    16261 ggcatcaagg ccgaaaaacg ctttgatatc aaagctcggg tagaacagcg cccgcgcgga
    16321 atccacctga tctaatgacg cctgaacata ccagcgcatg gcttgcagat ccgggcgtct
    16381 ggcgagcaac tcataagaga gtgtcgccgg aatgccggtc tggactcgtg gtaatgccac
    16441 cggtttgatc tccggcatat cgctggcccc cgcgccaatc aatgcacgca gagattctcg
    16501 cgtttcggtg atttgccctt tgacggcagc aatttgttta tcgaccgcca gaatctgtgc
    16561 ccgcgcgccg tggaaaggca cttgtgcttc cagaccgtgc gccactttac tctggtgcgc
    16621 tttcaccgcg taatcaatca catcgcgagt ttgttctaac agatcgagca tctgatagct
    16681 ggcctgcata ctgtaataaa gctgcgctac gcccgtggtc agcgatagct ctactgctgc
    16741 ggtttctgcc agcgcggcat tatgcgcgcc aatggcggcg gcaaccgctg agcgatgcac
    16801 accccacaaa tcaagatcca gtccggcaaa caaacctact gtggcttccg tatagtacgg
    16861 cccgtccata cccagtgcgg gcgcatccat cgcataaggg cttaaaaagc cgttcgccga
    16921 gacgcgttgg cggttgagca tccctaacgc tgcgacctgt aattgtgaac cggcatctaa
    16981 caaatcggcc tgggactgcg ctttttcttc ccgcagtttc gcttcggcga gggtgtgtga
    17041 accacttagc gtccgttgga tcagcgcatc cagctgcggg tcattgagtt gtttccacca
    17101 ctgcgcctgc ggccagccgg agctggcaag atgaatatca tcggccagtt tgatttgttc
    17161 cggtttgagc tgttgatgag gcgcagaatc cttgcgtacc aaggcacagc cggaaatcag
    17221 cgtcgtgctg ccgagaatgc tgcacaacag cagacgtgaa agttgacgat tgatcattgc
    17281 gtggctcctt gtgcctgttc cgtggcgggc gttaatgccg gggctgtcca gtctggcaag
    17341 cgggcgaaaa gctggcagac gtgttgctcc tgttttaata aggtgggcag gatggcctgc
    17401 gaggcgggtg tctcttcaag cgttatttga ggagaacggc tgagtgcggt tgccagaccg
    17461 gcagcgtatt tttccagggc gtcggcaaac tgacctgctc tgtccggctg tagtgcgtta
    17521 tccagcgcct gcgccgaatt ccaggtggca tcccaggcgt gaaggatatc gcggccctga
    17581 cgaataaccg tttgcgaacg ttcaatcagt aaggcgcgtt cttcgctgtc cagttgacgc
    17641 tccagcgcca cgcgttggca catctcttca caggcattaa acgccgcatg cagaccgata
    17701 cgaatttgca gataagtgcg cagagccgtg acttcctgct ggcgtgggat ccgcattact
    17761 ttacttaaca tacccagcgc gccagccagt ttttgcggca gcgtgcgcgc ttcactttca
    17821 ggccagacaa aggtgtaaat caccgcggac accacggtac caatgatgat acccagggcg
    17881 cgatcgcgaa tttccaccag gtcgtacact gggccaaaaa cgttttcgag cgtggcgagc
    17941 gcgaaggtga ccaccatctg tgtgccgata taagaagagc gttcagagct ggtggcaatc
    18001 catgcgccca acaggaaaat cggtgccagc acaaacagca attcgacaat attgtccagc
    18061 cagggcatga ccagtagcgt gaataacagc gccagaatcg cgccgcaaaa ggccccgcca
    18121 aaacgcagca ccatcttctg gtacgacgaa ccgacatttg gattagagac gatcacgcag
    18181 gtcagcatac aggtgtgaat gccttcccag tccacgccgc tgtaaaaggt gtaacagatc
    18241 aaacaggcga gcaatgtttt taccgcgtag cgtatatagt ctggattggt aaaagcatcg
    18301 gcgaccattg atggcggttt ggcggcgggc gttggcggcg tattcgggtt catctggccc
    18361 agttgtaaca acgtctggca gatattctcc aggttacatt cccgtgccgc caccgcttca
    18421 ctttcactga tccgccagtc gctttgccag cactgacctt cagcaacggc atgctgcagc
    18481 ttgttgattt ctgaggctaa tttttgccga aattcaataa ttgcctgaga atcagcaaaa
    18541 gaggtgggat cgtagcgatt cagcgtcgag taaatgtagg ttaccgttgc cacgcagctt
    18601 tgccaccatg cgctttgagt tcgccagttg gcatcgtccg cgagacaaaa gacattcagt
    18661 ttttgcagcg ccagcgcctc tctttcaatc cgcgtttcgg gtagcggtgc gaggctgtct
    18721 gtcaggtgac taatggcatc atcaagccga tcattaagcg cctgatgcat ttgcgaaatg
    18781 gcacgactgg gaaaccacag cacgccgatt aacgtcatca gcaaggttgg atagaggcca
    18841 acaacgatac accacagcgt taagcgcacg accacttccg gatagtcgag catggcgggg
    18901 aaggtttgcc cgtaaatggc gacaatggcg acagcgaaaa agaccagtcc cagccgatgg
    18961 gtgcgcatca aaaacatgcg gcccatcagg atcggcccgg cgatgatcaa tcggatcaac
    19021 ggttcgccgt atgaccattt gtagatcaaa aacaggctgc cgatctccag caccgtggca
    19081 accacaaaca agatcgccac aaatttggtg taaaacgcgt tcgactgaat accgtaaaac
    19141 agcactgcca gcgataacgc cacaaaaggg atctcaaaag tcatcgagat cagaatcacc
    19201 agcaggcagc ctacccagag ttgcacgatc tgcggcacgc ggccgggtcg ccgctcgctt
    19261 aactcttcat gaaagaacgc cagcagcctg accatcggta atggcaggga gttgagcgcg
    19321 ctcattattg cggctcaaga ttagcgactg ccgaagcgcc gatgcggaac atttccgggt
    19381 caggtttatc gaccatgatt ttgaccggaa aacgctgggc aacgcggacc cagttaatag
    19441 agcgagaaac tttcggcagg ccgcccagca ccaggccgcc gtcatccggt agcacgccgt
    19501 agccaatcga atccacttta ccctcgaagg ttttgccgct gtcactcatc aggcgaatcg
    19561 ttgcgggtgt acctgagcga atatttttca gatcggtttc gcggaagttg gcgatcacgt
    19621 accagtgacg agtgtcgatt agggtaaaaa tagggcgcat ggcagaagca aattgcccga
    19681 cggaggtttt gagggaaatg acccggccat caaacggcgc gcgaacggtc gccatttcca
    19741 gatgcagttt ggtcagggca atatccgctt cgaccgccgc acgttgcgcc accagcgcat
    19801 cgacgccgct gacggcgctg gcggctgact gcgcctgtaa caatacggca ttaagatcgg
    19861 cttctgcggc gcgctgcgca gttcttgcac ggtcaacgtc ttccgctgag acaaaaccct
    19921 ctttcagtaa tggctcggta cggcgtaatg tatctgtggc ctgtttcgcg gcggcacggg
    19981 ctttttctac cgtggcatta atcgagtcgg caccaaactg ttgcgcatca acgctacgct
    20041 gggtgagcat aatttgctta tccagcgccg cgagggaggc ttcagctttc gccagattgg
    20101 cttcgtacgg gcgcgggtca atgcggaaca gcaaatcgcc ctgtttgact gcctggttgt
    20161 cggtgaccgc cagttctaca atgcggccgc tgacttccgg caccacatca atggtatctg
    20221 ctgacgcgta agcgtcatta gttgatggcg cactgtctac gcgccagata acgaaaacaa
    20281 gggcaaccag cgccaatgca accactaaca gagcagggaa tttactgcga ggagcttttt
    20341 tcggcgtact ttccatgagc gcgtctcaaa tagattagaa aaatgccagc cagaacagca
    20401 tggcgtagag agcaaaaagg gcggtataaa taattccgac aaatgccaga ttgatatttg
    20461 cccgctgaat aactcgcctc gtgatgagca tcaaaataag gctggcaatg gcgcagaaaa
    20521 accagctggg ataataagca ccgatcaccg ggattgccgg agataatgag catcccgaaa
    20581 ccatgaagac gggaattatc caggctgttt ttttgagttg catcgccatg cgagagagaa
    20641 ctgttggcat attaaataca tcctgtctgc tgcgaacaat atctgtagtg aatatcaata
    20701 tccattgaag gcgtcagtat ggctattttc tcgatgaata aaaatagctt tattcagtc
//
LOCUS       DAEEKQ010000062        20721 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.062, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000062 DAEEKQ010000000
VERSION     DAEEKQ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.062"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(81..2903)
                     /gene="traG"
                     /locus_tag="KLK57_002177"
     CDS             complement(81..2903)
                     /gene="traG"
                     /locus_tag="KLK57_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer mating pair stabilization
                     protein TraG"
                     /protein_id="HBE0892540.1"
                     /translation="MNEVYVIAGGEWLRNNLNAIAAFMGTRTWDSIEKIALTLSVLAV
                     AVMWVQRHNVMDLLGWVAVFVLISLLVNVRTSVQIIDNSDLVQVHRVDNVPVGLAMPL
                     SLTTRIGHAMVASYEMIFTQPDSATYSKTGMLFGANLIVKSTDFLSRNPEIINLFQDY
                     VQNCVLGDIYLNHKYTLEDLMASADPYTLIFSRPSPLRGVYDSNNNFITCKDASVTLK
                     DRLNLDTKTGGKTWHYYVQQIFGGRPDPDLLFRQLVSDSYSYFYGSSQSASHIMRQNV
                     TMNALKEGITSNAARNGDTASLVSLATTSSMEKQRLAHVSIGHVLMRNLPMVQTILVG
                     ITIGIFPLLVLAAVFNRLTLSVLRGYVFALMWFQTWPLLYAILNSAMTFYAKQNGAPV
                     VLSELSQIQLKYSDLASTAGYISVMIPPLSWMMVKGLGAGFSSVYSHFASSAISPTAS
                     AAGSVVDGNYSYGNMQTENVNGFSWSTNSTTSFGQMMYQTGSGATATQTRDGNMVMDA
                     SGAMSRLPVGINATRQIAAAQQEMAREASNRAESALHGFSSSIASAWNSLSQFGSNRG
                     SSDSVTGGADSTMSAQDSMMASRMRSAVESYAKAHNISNEQATQELASRSTRASAGMY
                     GDAHAEWGVKPKILGVGGGLGVRGGGRAGIDWEDNDAHTASSNTQSSHNARHDIDARA
                     TQDFKEASDYFTSRKVSESGSHTDNNADSRVDQLSAALNSAKQSYDQYTTNMTRSHEY
                     AEMASRTESMSGQMSEDLSQQFAQYVMKHAPQDAEAILTNTSSPEIAERRRAMAWSFV
                     QEQVQPGVDNAWRESRGDIGKGMESVPSGGGSQDIIADHQGHQAIIDQRTQDSNIRND
                     VKHQVDNMVTEYKGNIGDTQNSVRGEENIVRGQYSELQNHHKTEALSQNNKYNEEKSA
                     QERMPGADSPQELMKRAKEYQDKYKQ"
     gene            complement(2900..4273)
                     /locus_tag="KLK57_002178"
     CDS             complement(2900..4273)
                     /locus_tag="KLK57_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraH"
                     /protein_id="HBE0892541.1"
                     /translation="MLRIKPLLVLCAALLTVTPAASADVNSDMNQFFNKLGFASNTTQ
                     PGVWQGQAAGYAYGGSLYARTQVKNIQLISMTLPDINAGCGGIDAYLGSFSFINGDQL
                     QRFVKQIMSNAAGYFFDLALQATVPEIKTAKDFLQKMASDINSMNLSSCQVAQGIVGG
                     LFPQTQVSQQKVCQDIAGESNIFADWAASRQGCTVGGQSSSVRDKASDKDKERVLKNI
                     NIIWDSLSKNRMLDGNKELKEFIMTLTGTLIFGEDSEITPLPARTTDRDILRAIMEGG
                     TAKVYHCNDSDKCLKVVADANVTIARDKALKSQITKLLTSIQNKAVSDTPLDDREKGF
                     ISSTTVPVFKYLIDPQMLGVSSSVVYQLTDYIGYDIMLQYIQELLQQARAMIATGNYP
                     QTVLDSVLENLNQAQQQIAVFQGQVKVQQDALLVVDRQMSYMRQQLSARMLSRYQNNY
                     HFGGSTL"
     gene            complement(4260..4685)
                     /gene="trbF"
                     /locus_tag="KLK57_002179"
     CDS             complement(4260..4685)
                     /gene="trbF"
                     /locus_tag="KLK57_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F-type conjugal transfer protein TrbF"
                     /protein_id="HBE0892542.1"
                     /translation="MNHRKYQRKLIMKEKRNDAELKNRKTKRDYDYERRVSDIYFDLF
                     FVFVAAGTFLWVIMHSIFDACIDSWKADPELNNFRYMWNILMYVIPYTLWAFAGGFLI
                     VYVRNPLNELINGGIRIFRLKRRMRREKKLREGGNNASH"
     gene            complement(4633..4980)
                     /gene="trbJ"
                     /locus_tag="KLK57_002180"
     CDS             complement(4633..4980)
                     /gene="trbJ"
                     /locus_tag="KLK57_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-type conjugative transfer protein TrbJ"
                     /protein_id="HBE0892543.1"
                     /translation="MPPVLWRGWIPFCRCTERKKVRNKQVVLLIAGISGIATGIIVSL
                     NIPFIRQGLFYPASPVEIVVSLCLTFSVSVVFFVGAIVGWISVSEIYYSRMNGLNESS
                     EISEETYNERKKK"
     gene            complement(4910..5455)
                     /gene="trbB"
                     /locus_tag="KLK57_002181"
     CDS             complement(4910..5455)
                     /gene="trbB"
                     /locus_tag="KLK57_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000052622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly thiol-disulfide isomerase TrbB"
                     /protein_id="HBE0892544.1"
                     /translation="MSLTKLLPVILLMMATGVQASTRDEIERLWNPQGMAVQPAQPAA
                     DTSARTEKPAPRWFRLSNGRQVNLADWKVVLFMQGHCPYCHQFDPVLKQLAQQYGFSV
                     FPYTLDGQGDTAFPEALPVPPDVMQTFFPNIPVATPTTFLVNVNTLEALPLLQGATDA
                     ASFMARMDTVLQMYGEEKGTK"
     gene            complement(5442..5726)
                     /gene="traQ"
                     /locus_tag="KLK57_002182"
     CDS             complement(5442..5726)
                     /gene="traQ"
                     /locus_tag="KLK57_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     chaperone TraQ"
                     /protein_id="HBE0892545.1"
                     /translation="MISKYRFSLPRLDITGMWVFSLGVWFHIVARLVYSKPWMAFFLA
                     ELIAAILVLFGAYQVLDAWIARVSLEEREALEARQQAMMEGQQEGGHVSH"
     gene            complement(5845..6189)
                     /gene="trbA"
                     /locus_tag="KLK57_002183"
     CDS             complement(5845..6189)
                     /gene="trbA"
                     /locus_tag="KLK57_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbA"
                     /protein_id="HBE0892546.1"
                     /translation="MHTGDLCFFTGASLFIVGVVGYFFTGPNNKGVYLLSSFVFFVVN
                     VISVCMLPDNFLFLCGAVMNQCAVPVVSMAVSTIPVITKFDSFRRMFVCIMLSSAWSG
                     VMWFFIRGLMTG"
     gene            complement(6203..6946)
                     /gene="traF"
                     /locus_tag="KLK57_002184"
     CDS             complement(6203..6946)
                     /gene="traF"
                     /locus_tag="KLK57_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TraF"
                     /protein_id="HBE0892547.1"
                     /translation="MNKALLPLLLCCFIFPASGKDAGWQWYNEKINPKEKENKPVPAA
                     PRQEPDIMQKLAALQTATKRALYEAILYPGVDNFVKYFRLQNYWTQQAGLFTMSAKKA
                     MLAHPELDYNLQYSHYNGTVRNQLAADQAQQRQAIAKLAEHYGIMFFYRGQDPIDGQL
                     AQVINGFRDTYGLSVIPVSVDGVINPLLPDSRTDQGQAQRLGVKYFPAMMLVDPKQGS
                     VRPLSYGFISQDDLAKQFLNVSEDFKPNF"
     gene            complement(6939..7196)
                     /gene="trbE"
                     /locus_tag="KLK57_002185"
     CDS             complement(6939..7196)
                     /gene="trbE"
                     /locus_tag="KLK57_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbE"
                     /protein_id="HBE0892548.1"
                     /translation="MKVIFTSNRLVDFLIRLSITAIVISPVIIWSWDTVKETTADGML
                     AAAFVILYSGVLLFILYFCFSALTGLQKDDERKNDERNEDE"
     gene            complement(7223..9073)
                     /gene="traN"
                     /locus_tag="KLK57_002186"
     CDS             complement(7223..9073)
                     /gene="traN"
                     /locus_tag="KLK57_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system mating-pair
                     stabilization protein TraN"
                     /protein_id="HBE0892549.1"
                     /translation="MKRILPLILALVAGMAQADSNSDYRAGSDFARQIQGQGTGSIQG
                     FKPQESIPSYNANPDETKYYGGVTAGGDGGLKNDGTTEWATGETGKTITESFMNKPKD
                     ILSPDAPFIQTGRDVVNRADSIVGNTGQQCSAQEINRSEFTNYTCERDTMVEEYCTRT
                     ASITGDWKYTDEYREVTIPHSQFRFSMNGLKLVFSVTAPVTGTVESASLSVYAAFFFL
                     NSRYTFMNTTFNVGLANGQSDTYPLSGATGLQVTQGQVLTGSGCTANGNCLPHGNGDR
                     KVYESLVSGASTFTLKLRMKVRDKEWVPRVEWVESCPFNKADGVLTGTECSEPGGTKT
                     GVMEGKPWNITQACWAYRDKYVTQSADNGTCQKYVDNPACTLASRQCAFYSDEGTCLH
                     EYATYSCESRTSGKVMVCGGDVFCLDGECDKAQSGKSSDFGEAVSQLAALAAAGKDVA
                     ALNGVDVRAFTGEAKFCRKAAAGFSNCCKDGGWGQDVGLAKCNSEEKALGKAKDNKLT
                     VSVGEFCSKKVLGVCLQKKRSYCQFDSKLAQIVQQQGRNGQLRIGFGSAKHPDCRGIT
                     VDELQKIQFDRLDFTNFYEDLMNNQKIPDSGVLTQKVKEQIADQLKQAGQ"
     gene            complement(9070..9708)
                     /gene="trbC"
                     /locus_tag="KLK57_002187"
     CDS             complement(9070..9708)
                     /gene="trbC"
                     /locus_tag="KLK57_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001733892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system pilin
                     assembly protein TrbC"
                     /protein_id="HBE0892550.1"
                     /translation="MNRKIFALFALTAGMHLAVHASENVNTTENRQFLKQQENLSRQL
                     REKPDHQLKAWAEKQVLENPLQRSDNHFLDELVRKQQASQDGKPRQGALYFVSFSIPE
                     EGLKRMLGETRHYGIPATLRGMVNNDLKTTAEAVLSLVKDGATDGVQIDPTLFSQYGI
                     RSVPALVVFCSQGYDIIRGNLRVGQALEKVAATGDCRQVAHDLLAGKGDSGK"
     gene            complement(9717..10022)
                     /locus_tag="KLK57_002188"
     CDS             complement(9717..10022)
                     /locus_tag="KLK57_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892551.1"
                     /translation="MATMDSKYIQHLRKISAVLLRLISRTLKLFPFGLVIVPVILFYF
                     APDSFLAEIIHEWKNADISEQVTLVRLYLKVTFTCAFVAVFLNYLFSDEFMPVNSRK"
     gene            complement(10052..11044)
                     /locus_tag="KLK57_002189"
     CDS             complement(10052..11044)
                     /locus_tag="KLK57_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus assembly protein TraU"
                     /protein_id="HBE0892552.1"
                     /translation="MKRSLWLLMLFLLAGHVPAASADSACEGRFVNPITDICWSCIFP
                     LSLGSIKVSQGKVPDTANPSMPIQICPAPPPLFRRIGLAIGYWEPMALTDVTRSPGCM
                     VNLGFSLPAFGKTAQGTAKKDEKQVNGAFYHVHWYKYPLTYWLNIITSLGCLEGGDLD
                     IAYLSEIDPTWTDSSLTTILNPEAVIFANPIAQGACAADAIASAFNMPLDVLFWCAGS
                     QGSMYPFNGWVSNESSPLQSSLLVSERMAFKLHRQGMIMETIGKNNAVCNEYPSPILP
                     KERWRYQMVNMYPDSGQCHPFGRSVMRWETGKNPPNTKKNFGYLMWRKRNCVFL"
     gene            complement(11041..11673)
                     /gene="traW"
                     /locus_tag="KLK57_002190"
     CDS             complement(11041..11673)
                     /gene="traW"
                     /locus_tag="KLK57_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TraW"
                     /protein_id="HBE0892553.1"
                     /translation="MRCRGLIALLIWGQSVAAADLGTWGDLWPVKEPDMLTVIMQRLT
                     ALEQSGEMGRKMDAFKERVIRNSLRPPAVPGIGRTEKYSSRLFDPSVRLAADIRDNEG
                     RVFARQGEVMNPLQYVPFNQTLYFINGDDPAQVAWMKRQTPPTLESKIILVQGSIPEM
                     QKSLDSRVYFDQNGVLCQRLGIDQVPARVSAVPGDRFLKVEFIPAEEGRK"
     gene            complement(11670..12056)
                     /gene="trbI"
                     /locus_tag="KLK57_002191"
     CDS             complement(11670..12056)
                     /gene="trbI"
                     /locus_tag="KLK57_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system protein TrbI"
                     /protein_id="HBE0892554.1"
                     /translation="MTTTQKTTDVTAPRRSHWWWTVPGCLAMVLLNAAVSYGIVRLNA
                     PVTAAFNMKQTVDAFFDSASQKQLSEAQSKALSARFNTALEASLQAWQQKHHAVILVS
                     PAVVQGAPDITREIQQDIARRMRAEP"
     gene            complement(12053..14683)
                     /gene="traC"
                     /locus_tag="KLK57_002192"
     CDS             complement(12053..14683)
                     /gene="traC"
                     /locus_tag="KLK57_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000069745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein TraC"
                     /protein_id="HBE0892555.1"
                     /translation="MSNNPLEAVTQAVNSLVTALKLPDESAKANEVLGEMSFPQFSRL
                     LPYRDYNQESGLFMNDTTMGFMLEAIPINGANKSIVEALDHMLRTKLPRGIPLCIHLM
                     SSQLVGDRIEYGLREFSWSGEQAERFNAITRAYYMKAAATQFPLPEGMNLPLTLRHYR
                     VFISYCSPSKKKSRADILEMENLVKIIRASLQGASIATQTVDAQAFIDIVGEMINHNP
                     DSLYPKRRQLDPYSDLNYQCVEDSFDLNVRADYLTLGLRENGRNSTARILNFHLARNP
                     EIAFLWNMADNYSNLLNPELSISCPFILTLTLVVEDQVKTHSEANLKYMDLEKKSKTS
                     YAKWFPSVEKEAKEWGELRQRLGSGQSSVVSYFLNITAFCKDNNETALEVEQDILNSF
                     RKNGFELISPRFNHMRNFLTCLPFMAGKGLFKQLKEAGVVQRAESFNVANLMPLVADN
                     PLTPAGLLAPTYRNQLAFIDIFFRGMNNTNYNMAVCGTSGAGKTGLIQPLIRSVLDSG
                     GFAVVFDMGDGYKSLCENMGGVYLDGETLRFNPFANITDIDQSAERVRDQLSVMASPN
                     GNLDEVHEGLLLQAVRASWLAKENRARIDDVVDFLKNASDSEQYAGSPTIRSRLDEMI
                     VLLDQYTANGTYGQYFNSDEPSLRDDAKMVVLELGGLEDRPSLLVAVMFSLIIYIENR
                     MYRTPRNLKKLNVIDEGWRLLDFKNRKVGEFIEKGYRTARRHTGAYITITQNIVDFDS
                     DKASSAARAAWGNSSYKIILKQSAKEFAKYNQLYPDQFQPLQRDMIGKFGAAKDQWFS
                     SFLLQVENHSSWHRLFVDPLSRAMYSSDGPDFEFVQQKRKEGLSIHEAVWQLAWKKSG
                     PEMASLEAWLEEHEKYRSVA"
     gene            complement(14809..15156)
                     /locus_tag="KLK57_002193"
     CDS             complement(14809..15156)
                     /locus_tag="KLK57_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000836682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892556.1"
                     /translation="MKSIYLKSVLAFIFVGVMAMIVCIPFYIVYLAQQPATPEQLTEI
                     LQETPCAAEAFQETLNYQSEPLTLGKANKIASECRKRNEMAEVKRVRENERNKIREKQ
                     IQALNDAHSVKER"
     gene            complement(15184..15402)
                     /locus_tag="KLK57_002194"
     CDS             complement(15184..15402)
                     /locus_tag="KLK57_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892557.1"
                     /translation="MHTFIFLVLIKNINGGGSWNITPMPNTDVCVQVLRTIEAHGGRG
                     FGNTGECIEVKTNEPLNSVMETLNGGGQ"
     gene            complement(15482..15958)
                     /locus_tag="KLK57_002195"
     CDS             complement(15482..15958)
                     /locus_tag="KLK57_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000549587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892558.1"
                     /translation="MHKSENEKIDFILDEHERISRKQGSLCMALGKGFLVIAILCGVA
                     TFCVSGLYLKSLCLSVACCCGVFSRIFNYYSVPEESRMVLSRQELLRLMRLTEDVPDV
                     RQELLNRLLSGKKLTGRDEQDIRRLWQEKVDAMQESATRQREQDTIRKFTEGNKSE"
     gene            complement(15951..16172)
                     /gene="traR"
                     /locus_tag="KLK57_002196"
     CDS             complement(15951..16172)
                     /gene="traR"
                     /locus_tag="KLK57_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraR"
                     /protein_id="HBE0892559.1"
                     /translation="MSDEADEAYSVTEQLTMTGINRIRQEINVHGIPVYLCEACGNPI
                     PEARRKIFPGVTLCVECQAYQERQRKHYA"
     gene            complement(16307..16822)
                     /gene="traV"
                     /locus_tag="KLK57_002197"
     CDS             complement(16307..16822)
                     /gene="traV"
                     /locus_tag="KLK57_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system lipoprotein
                     TraV"
                     /protein_id="HBE0892560.1"
                     /translation="MKQISLFIPLLGTLLLSGCAGTNTEFECNATTSDTCMTMEQANE
                     KAKKLEQSSEAKPVAASLPRLAEGNFRTMPVQTVTATTPSGSRPAVTAHPEQKLLAPR
                     PLFTAAREVKTVVPVSSVTPVTPPRPLRTGEQTAALWIAPYIDNQDVYHQPSSVFFVI
                     KPSAWGKPRIN"
     gene            complement(16819..17187)
                     /locus_tag="KLK57_002198"
     CDS             complement(16819..17187)
                     /locus_tag="KLK57_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TrbD"
                     /protein_id="HBE0892561.1"
                     /translation="MFISLMTEQTEATEIKMNMRNINVITARVVPDKNVSDDFMHAVL
                     SNCTTKIVLPAPKVFSSESLPHNFNMAAVGVMKKGMSMSLFSSPGNTVVSGHPCAGKT
                     NLTAELISAVVGSNKKGEGQ"
     gene            complement(17126..17716)
                     /locus_tag="KLK57_002199"
     CDS             complement(17126..17716)
                     /locus_tag="KLK57_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001528624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraP"
                     /protein_id="HBE0892562.1"
                     /translation="MANNMSSRQACHAARYVVARVLRGLFWCLKYTVILPLATMALMA
                     LFVLWKDNTTPGKLLVKEINFVRQTAPAGQFPVSECWFSSSDSSGRSEIQDICHYRAA
                     DAADYVRETDRSLMQLVTAFWATLALMYMSFAAGTGKYPVLPGKMKCIRVVTADEYLK
                     DVYTEDASLPGKIRKCHVYFPDDRTNRSNGDKNEHA"
     gene            complement(17706..19133)
                     /locus_tag="KLK57_002200"
     CDS             complement(17706..19133)
                     /locus_tag="KLK57_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001553749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraB"
                     /protein_id="HBE0892563.1"
                     /translation="MASINTIVKRKQYLWLGIVVVGTASAIGGALYLSDVDMSGNGET
                     VAEQEPVPDMTGVVDTTFDDKVRQHATTEMQVTAAQMQKQYEEIRRELDVLNKQRGDD
                     QRRIEKLGQDNAALAEQVKALGANPVTATGEPVPQMPAPPPGPEGEPQPGNTPVSFPP
                     QGSVAVPPPTAFYPGNGVTPPPQVTYQSVPVPNRIQRKVFTRNEGKQGPSLPYIPSGS
                     FAKAMLIEGADANASVTGNESTVPMQLRITGLVEMPNSKTYDATGCFVGLEAWGDVSS
                     ERAIVRTRNISCLKDGKTIDMPIKGHVSFRGKNGIKGEVVMRNGKILGWAWGAGFVDG
                     IGQGMERASQPAVGLGATAAYGAGDVLKMGIGGGASKAAQTLSDYYIKRAEQYHPVIP
                     IGAGNEVTVVFQDGFQLKTVEEMALERTQSRAEEDNPESPVPVPPSAESHLNGFNTDQ
                     MLKQLGNLNPQQFMSGSQGGGNDGK"
     gene            complement(19133..19861)
                     /gene="traK"
                     /locus_tag="KLK57_002201"
     CDS             complement(19133..19861)
                     /gene="traK"
                     /locus_tag="KLK57_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type-F conjugative transfer system secretin
                     TraK"
                     /protein_id="HBE0892564.1"
                     /translation="MRKNNTAIIFGGLFFSCSVMAANGTLAPTVVPMVNGGQASIAIS
                     NTSPNLFTVPGDRIIAVNSLDGALTNNEQTASGGVVVATVNKKPFTFILETERGLNLS
                     IQAVPREGAGRTIQLVSDLRGTGEEAGAWETSTPYESLLVTISQAVRGGKLPAGWYQV
                     PVTKETLQAPAGLSSVADSVWTGNHLKMVRFVVENKTLSALNIRESDFWQPGTRAVMF
                     SQPASQLLAGARMDVYVIRDGEGN"
     gene            complement(19848..20414)
                     /gene="traE"
                     /locus_tag="KLK57_002202"
     CDS             complement(19848..20414)
                     /gene="traE"
                     /locus_tag="KLK57_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraE"
                     /protein_id="HBE0892565.1"
                     /translation="MEHGARLSTSRVMAIAFIFMSVLIVLSLSVNLIQGVNNYRLQNE
                     QRTAVTPMGFNVPFAVSQNSADASYLQQMALSFIALRLNVSPEIVDASHQALLQYIRP
                     GAQNQMKVILAEEAKLIKKDNVNSAFFQTSVRVWPQYGRVEIRGIRKTWIGNSEPFTD
                     IKHYILILKRENGVTWLDNFGETDDEKK"
     gene            complement(20436..>20721)
                     /gene="traL"
                     /locus_tag="KLK57_002203"
     CDS             complement(20436..>20721)
                     /gene="traL"
                     /locus_tag="KLK57_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type IV conjugative transfer system protein
                     TraL"
                     /protein_id="HBE0892566.1"
                     /translation="KYRFPETLTNQSRWFGLPLDELIPAAICVGWGITTSKYLFGIGA
                     AVLVYFGIKKLKKGRGSSWLRDLIYWYMPTALLRGIFHNVPDSCFRQWIK"
BASE COUNT         4907 a         5766 c         4857 g         5191 t
ORIGIN      
        1 agataacagg cgataaactg tatcaccgtt accagagtaa tatgggttaa acatctcatt
       61 atcatctccc aaaaacagca ttattgctta tacttatcct gatattcctt tgcccttttc
      121 atcagttcct gtggactgtc agccccgggc attctttcct gagccgactt ttcttcatta
      181 tatttattat tctgagataa tgcctctgtt ttatggtgat tctgcagttc agaatactgt
      241 cctcttacaa tattttcctc gccacgtacg ctgttctgag tatcacctat attcccttta
      301 tattctgtga ccatattatc aacctgatgt tttacatcat tacggatatt actgtcctgc
      361 gttctttgat caataatggc ctgatgcccc tgatggtcag caataatatc ctgactgcca
      421 ccacccgaag gtacgctctc cattccttta ccaatatcac cacgggattc ccgccaggca
      481 ttatcaacac caggctgtac ctgttcctgc acaaaagacc aggccatcgc ccgacgacgt
      541 tctgcaatct ccggtgaact ggtgtttgtc agaatagctt ctgcatcctg tggcgcgtgt
      601 ttcattacat actgtgcaaa ctgttgcgac aggtcttcac tcatctgccc actcatgctt
      661 tcagtacggg atgccatttc agcatattca tggctgcggg tcatattcgt cgtgtactgg
      721 tcgtaactct gcttcgctga attcagggct gcagacaact gatccacacg ggaatcagca
      781 ttattgtccg tatggctgcc ggactcactg accttgcgac tggtaaaata atcgctggcc
      841 tctttaaagt cttgtgtagc tctggcatcg atatcatgac gagcattatg gctcgactga
      901 gtattactac ttgccgtatg cgcatcatta tcttcccaat caataccagc ccgaccacca
      961 cctctaacgc ctaatcctcc accaacacca agaattttag gtttaacccc ccattcagca
     1021 tgagcatctc catacatccc ggcagaagct ctcgtacttc tggatgccag ttcctgagtc
     1081 gcctgctcat tactgatatt atgcgccttc gcatagcttt ccactgcact gcgcatacgg
     1141 ctggccatca tggagtcctg tgcgctcatc gtgctgtcag caccacctgt gacagaatca
     1201 ctgctccccc ggttagaacc aaactggctg agcgagttcc aggcactggc aatactgctg
     1261 ctgaacccat gcagggcgct ttctgctctg ttcgacgcct cccgggccat ttcctgttgt
     1321 gccgctgcaa tctgacgcgt tgcattgata ccgaccggta accgggacat cgccccgctc
     1381 gcatccatca ccatattacc gtcacgggtc tgtgtggcag ttgcgccact gccggtctgg
     1441 tacatcatct gaccaaacga cgtggtgctg ttggtgctcc agctgaagcc gttcacgttt
     1501 tccgtctgca tgttgccgta ggagtaatta ccgtcaacca cactacctgc cgcactggca
     1561 gtcgggctga tagcagagga agcgaaatgg ctgtacacgc tggaaaaacc tgcgccaagg
     1621 ccttttacca tcatccatga caacgggggt atcatgacag atatatatcc ggcagtggac
     1681 gccaggtcgg agtatttcag ctgtatctga gagagttcag agagcacgac cggtgcacca
     1741 ttctgctttg catagaatgt catggcactg ttcagaatgg catacagcaa cggccaggtc
     1801 tggaaccaca tcagagcaaa cacatagcct ctcagaacag acagcgtcag tctgttgaag
     1861 acagccgcca gtaccaggag cgggaagata ccaatggtaa tgcccaccag aatggtctgc
     1921 accatcggca ggtttcgcat cagcacatga ccaatggaga catgtgccag acgctgtttc
     1981 tccatcgacg aagtcgtggc cagactgacc aggctggcgg tatcaccgtt acgggctgca
     2041 ttactggtga taccctcttt cagggcattc atggtgacgt tctggcgcat aatatgactg
     2101 gcagactggc tggagccgta gaaataactg taactgtcac tcaccagttg cctgaataac
     2161 aggtccgggt ccggtctgcc gccaaatatc tgctgcacat aataatgcca ggtcttgcct
     2221 cccgtctttg tatcgagatt cagcctgtct ttcagcgtga ccgacgcatc tttacaggtt
     2281 atgaaattat tattgctgtc ataaacgccc cgtagcgggc tgggccggga aaaaatcagc
     2341 gtgtaggggt cagcggaggc catcaggtct tccagcgtgt atttgtggtt caggtaaata
     2401 tcccccagca cgcagttctg gacgtagtcc tgaaacagat tgatgatttc cggattccgg
     2461 gacagaaaat cggtgctttt cacaatcagg ttcgccccga acagcatgcc tgtcttgctg
     2521 taggtggcgc tgtcaggctg ggtgaatatc atctcgtaac tggccaccat tgcatgcccg
     2581 atacgggtcg tcagtgaaag tggcatcgcc agtccgacag gcacattatc cacccggtga
     2641 acctggacca ggtcactgtt atcaataatc tgcaccgatg tacggacatt aaccagcagg
     2701 ctgataagca caaacacggc cacccagccc agcaaatcca tcacgttgtg ccgctgtacc
     2761 cacatcacgg ccaccgcgag aacagacaat gtgagcgcaa ttttttcgat ggaatcccac
     2821 gtccgggtcc ccataaaggc ggcaatggcg ttcaggttat tgcgtaacca ctcaccaccg
     2881 gcaatcacat agacttcatt cacagcgtgc tccctccgaa gtgatagttg ttctggtaac
     2941 ggctgagcat gcgggcggaa agctgctggc gcatatagct catctgacgg tcaacaacca
     3001 gcaatgcatc ctgctggacc tttacctgcc cctggaaaac cgcaatctgt tgctgtgcct
     3061 gattcagatt ctccagcaca ctgtccagca cggtctgtgg atagttcccg gtggcaatca
     3121 tggcccgggc ctgctgaagc agttcctgaa tgtactggag catgatgtcg tagccgatat
     3181 agtccgtcag ctggtaaacc acgctgctgg atacaccgag catctgcggg tcaataagat
     3241 atttaaagac ggggacggtg gtgctggaaa tgaagccttt ttccctgtca tccagtggag
     3301 tatcactcac cgctttattc tgaatactgg tgagcagttt tgtaatctgt gatttcaggg
     3361 ctttatcccg ggcgatggtc acatttgcat ccgccaccac cttcagacac ttgtccgaat
     3421 cgttgcagtg ataaactttt gccgttcccc cctccataat cgcccggagg atatcgcggt
     3481 cagtggtacg cgccggcagg ggggtaatct cgctgtcttc cccaaaaatc agcgtaccgg
     3541 tgagcgtcat aataaattct ttcagttctt tgttaccatc aagcatcctg tttttggaca
     3601 gcgagtccca gatgatattg atgttcttca gcacccgttc cttatctttg tcactggcct
     3661 tgtcacgaac agacgatgac tgtcctccga ccgtgcagcc ctgacgggac gcggcccagt
     3721 cagcaaaaat attgctctca ccggcaatgt cctgacagac cttctgctgt gacacctgag
     3781 tctgaggaaa taatcctccg acaatcccct gcgccacctg acaggagctg aggttcatac
     3841 tgttaatgtc gcttgccatt ttctgcagga agtcttttgc ggttttgatt tccggcaccg
     3901 ttgcctgcag ggcaaggtca aaaaagtaac cggcagcgtt gctcataatc tgcttaacaa
     3961 aacgctgcaa ctgatcgcca ttaataaaac tgaacgagcc gagataggcg tcgatgccgc
     4021 cacatccggc attgatatcc ggcagcgtca tggaaatcag ctggatattt ttgacctggg
     4081 tgcgggcata cagggagcca ccataggcat acccggcggc ctgcccctgc cagacgcccg
     4141 gttgtgtggt attggaagca aatcccagct tattaaagaa ctgattcatg tcgctgttca
     4201 catccgctga cgctgcgggt gtgaccgtca gtaaagcggc acaaagaacc agaagaggtt
     4261 taatgcgaag cattattgcc accctccctt aatttcttct cccggcgcat acggcgtttc
     4321 agccggaata ttctgatccc cccgtttatt aattcattta aagggttcct gacgtatacg
     4381 ataagaaatc cacctgcaaa cgcccataac gtatacggaa tgacatacat caggatgttc
     4441 cacatataac gaaaattatt taattccggg tcagctttcc acgaatcaat acaggcatca
     4501 aaaatactat gcataatcac ccataaaaaa gttccggcag caacgaacac aaaaaacaga
     4561 tcaaaatata tatcagaaac cctgcgttca taatcataat cccgtttagt ttttctgttt
     4621 tttagttccg catcatttct tttttctttc attataagtt tcctctgata tttccgatga
     4681 ttcattcaga ccgttcatgc ggctgtaata tatttcagac accgaaatcc atcccacaat
     4741 tgcccccaca aaaaacacaa cagaaacaga aaaggtaaga cacagcgaaa cgacaatttc
     4801 cacagggctg gcgggataaa aaagcccctg acggataaag gggatattca ggctgacgat
     4861 tattcccgtc gcgatgccgg atattccggc aatgagtaac accacctgct tatttcgtac
     4921 ctttttcctc tccgtacatc tgcagaacgg tatccatccg cgccataaaa ctggcggcat
     4981 ccgttgcccc ctgcaaaagc ggtaatgcct caagtgtgtt gacgttgacc aggaaggtag
     5041 tcggtgtggc caccgggata ttcgggaaga aggtctgcat cacgtccggt ggcaccggta
     5101 atgcttccgg aaaggcggta tccccctgac catccagggt gtagggaaaa acggaaaagc
     5161 cgtactgctg tgccagctgt ttcagtaccg ggtcaaactg gtgacagtaa gggcaatgcc
     5221 cctgcataaa cagcaccacc ttccagtcgg ccaggttaac ctgcctgccg ttgctgaggc
     5281 ggaaccagcg gggagccggc ttttctgttc tggctgatgt gtctgctgcc ggttgtgcag
     5341 gctgcacggc cataccctgc ggattccaga gtcgttcaat ttcatcccgg gtggaggcct
     5401 gcacacctgt ggccatcatc aacaaaatga ccggcagtaa tttagtgaga gacatgtccg
     5461 ccctcctgct gcccttccat catggcctgt tgcctggctt ccagtgcctc acgctcttcc
     5521 aggctgacac gtgcaatcca tgcatccagc acctggtatg cgccgaacag cacaaggatg
     5581 gccgcaatca actccgccag aaaaaaagcc atccagggtt tgctgtaaac aagacgggcg
     5641 acgatatgaa accagacacc cagggaaaat acccacattc ccgtaatatc aagccggggt
     5701 aaagagaatc tgtatttact tatcatgtta aactccggtt attatttaca tgagagtatt
     5761 catgtctgta atattcgcgt ttgtgtgata ttaatcagat caacctcatt attttgtatg
     5821 gtttttatta ccgttttata tgacttagcc tgtcataagc ccccttataa aaaaccacat
     5881 cactccggac catgctgatg acaacataat acaaacaaac atccttctga aactgtcaaa
     5941 cttcgtgata accggtatgg tactgactgc cattgaaaca accggtacgg cacactgatt
     6001 cataaccgca ccacaaagaa acaagaaatt atctggcagc atacatacac ttatgacatt
     6061 tactacaaag aagacaaacg agctcagcag ataaactcct ttattatttg gtcctgtaaa
     6121 gaaataacca acaaccccca cgataaacag gctggcacct gtaaaaaaac acaaatcccc
     6181 tgtatgcata tataaccttt ggttaaaaat tcggtttaaa atcttcagaa acgttcagga
     6241 actgttttgc caggtcgtcc tgtgaaataa agccgtatga taacgggcga acactgccct
     6301 gtttcgggtc aaccagcatc atggccggga aatatttcac accgaggcgc tgcgcctgcc
     6361 cctggtcagt ccgggaatcc ggcaacagcg gattaatcac gccatccacg gaaacgggaa
     6421 taacactcag accataggta tccctgaagc cattaatgac ctgcgccagt tgcccgtcga
     6481 tggggtcctg cccccggtaa aagaacatga tgccgtagtg ttcagccagt ttcgcaatgg
     6541 cctgtcgctg ctgcgcctgg tctgccgcca gctggttccg taccgtgccg ttgtaatggc
     6601 tgtactgcag gttatagtcc agttcgggat gtgccagcat ggccttttta gcactcatgg
     6661 tgaaaagccc ggcctgctga gtccagtaat tctgcagccg gaaatattta acaaaattat
     6721 ccacgccggg atacagaatg gcttcgtaca gcgcccgctt tgttgccgtc tgcagcgcgg
     6781 ccagtttctg cataatatcc ggctcctgac ggggggctgc aggtacaggt ttattttctt
     6841 tttccttcgg atttattttc tcgttatacc actgccagcc tgcatctttc cctgacgccg
     6901 gaaaaataaa gcagcagagt aacagtggca gtaatgcttt attcatcttc attcctttca
     6961 tcattttttc tttcatcatc tttctgcagg ccagtcaggg cagaaaaaca aaaatacaga
     7021 ataaacagga gcacaccgga gtaaaggata acgaaagcag cggcaagcat cccgtctgcc
     7081 gtggtttctt ttaccgtatc ccacgaccag ataattaccg gggaaatgac aatcgctgta
     7141 atcgaaagcc ggataagaaa atcaacaaga cgattagaag tgaatataac tttcatcatt
     7201 tcccccggtt attttccata ccttactgtc ctgcctgttt cagctggtca gcaatctgct
     7261 ctttcacttt ctgcgtcaga acgccactgt ccgggatttt ctggttattc atcaggtctt
     7321 cgtagaagtt agtgaaatcc agtctgtcga actgaatttt ctgcagctca tcaaccgtaa
     7381 tcccccgaca gtcaggatgc ttcgcactgc caaacccgat acgcaactgc ccgttacgcc
     7441 cctgctgctg gactatctgc gccagcttcg aatcaaactg gcagtagctg cgctttttct
     7501 gcagacagac acccagcact ttcttcgaac agaactctcc gacactgacg gtcagtttgt
     7561 tgtcctttgc ctttcccagc gccttttctt cgctgttgca cttcgccaga cccacatcct
     7621 gaccccagcc accatcctta cagcagttac tgaagccggc tgcggcctta cggcagaatt
     7681 tcgcctcacc cgtgaaagcc ctgacatcca caccattcag ggcggcaaca tccttacctg
     7741 cggcggcaag cgcagccagc tgtgatactg cttcaccaaa atcactgctt tttccgctct
     7801 gtgccttatc gcattcaccg tcaaggcaga agacatcacc accacagacc atcacttttc
     7861 ctgatgttct ggactcgcag gagtacgtgg cgtactcatg cagacaggtt ccctcatcgg
     7921 aataaaacgc gcactgacgt gaggccagcg tacaggccgg attatcgaca tatttctgac
     7981 aggtgccgtt atctgcggac tgagtgacat atttatcccg gtatgcccag catgcctgtg
     8041 tgatgttcca gggtttacct tccatcactc cggttttcgt accacccggc tctgaacact
     8101 cagtgcctgt cagcacgcca tccgctttat taaacggaca actctccacc cactccaccc
     8161 ggggaaccca ttctttatca cggaccttca tcctgagttt cagcgtaaag gtggaagcac
     8221 cactgacaag cgattcatag actttcctgt caccattccc gtgcgggagg caattaccgt
     8281 tagcagtaca gccactaccg gtcagaacct gcccctgtgt cacctgcaaa cctgtcgccc
     8341 cggacagggg gtaagtatcc gactgcccgt ttgccaggcc gacattaaag gttgtattca
     8401 tgaaggtata acgggagttc aggaagaaaa aagcggcgta tacagacaat gatgcacttt
     8461 ccactgtacc ggttaccgga gccgtgacac tgaacaccag tttcagcccg ttcatactga
     8521 accggaactg tgagtgcggt atcgtcactt cacgatattc atccgtgtat ttccagtccc
     8581 cggtgatact ggcagtccgt gtgcagtact cctccaccat cgtgtcccgc tcacaggtat
     8641 agtttgtgaa ttcactgcgg tttatctcct gcgcactgca ctgctgtccg gtatttccca
     8701 caatgctgtc agcccggttc accacatccc tgccggtctg aatgaacggt gcatccggtg
     8761 acagaatgtc tttcggcttg ttcatgaagg actctgtgat ggtttttccg gtttcaccgg
     8821 tcgcccattc cgtggtgccg tcatttttca ggccgccatc cccgccggca gtcacgccac
     8881 cgtaatattt tgtctcgtcc ggattcgcgt tatagctggg gatactctcc tgtggcttaa
     8941 agccctgaat actgcctgta ccctgcccct gaatctgacg ggcaaaatca gaaccggccc
     9001 ggtaatcact gttgctgtca gcctgtgcca tgccggcaac cagagccagt atcagaggta
     9061 aaatacgttt catttcccgg aatctccttt ccctgccagt aaatcatgag ccacctgccg
     9121 gcagtcaccc gtggcggcca ctttctccag cgcctggccg acccgcaggt ttccccggat
     9181 gatgtcgtat ccctgactgc agaacaccac cagtgccggt acactgcgaa tgccgtactg
     9241 tgaaaacagc gtcgggtcga tctgaacacc atcagtcgcg ccgtctttca ccagggacag
     9301 cacggcttca gcggtggtct tcaggtcatt gttcaccatg ccccgcagtg tggcgggtat
     9361 accgtagtgc cgggtttcgc ccagcatccg tttcagcccc tcttcgggaa tggaaaacga
     9421 cacaaaatac agggcaccct gccggggttt cccgtcctgc gaagcctgct gtttacgaac
     9481 cagctcatcc agaaaatggt tatctgaacg ctgaaggggg ttttccagca cctgtttctc
     9541 cgcccaggct ttcagctgat ggtcaggttt ctctcgcagt tgtctgctta aattttcctg
     9601 ctgcttcagg aactggcggt tttcagttgt gttcacattt tctgaagcat gaacagcaag
     9661 atgcatccct gctgtcagag caaaaagtgc aaatattttc cggttcatga tatttctcac
     9721 tttctgctat ttacaggcat gaactcatca gaaaaaagat aattgagaaa aacagccaca
     9781 aacgcacatg taaatgtcac cttcagatat aatctgacaa gtgtcacttg ttctgatata
     9841 tctgcgttct tccattcatg aataatttca gcgagaaaac tgtcaggtgc aaaataaaat
     9901 aatataacgg gcactattac aagaccaaaa ggaaataatt ttaatgttct gcttatcagc
     9961 ctgagcagta cagcacttat tttcctgagg tgctgaatat atttactgtc cattgttgcc
    10021 attttcattt cctcttattc attaaaatga tttacaggaa tacacagtta cgtttacgcc
    10081 acatcaggta gccgaagttt ttctttgtgt tgggcggatt ttttcccgtc tcccagcgca
    10141 tcacactgcg cccgaacgga tggcactgtc cgctgtccgg atacatattc accatctgat
    10201 aacgccagcg ttctttaggc aggattgggg acggatattc attacagacg gcgttatttt
    10261 tcccgatggt ttccataatc atgccctgac ggtgtagttt gaacgccatg cgttcactga
    10321 ccagcaggga ggactgcaac ggactggact cattactcac ccagccattg aacgggtaca
    10381 tactgccctg cgaaccggca caccagaaca gaacatcgag aggcatatta aaggcgctgg
    10441 caatggcatc tgctgcacag gccccctgtg ctatcggatt ggcaaagatg acagcttccg
    10501 gattgagaat ggtggtcagg ctgctgtccg tccaggtggg gtcaatttca gaaagataag
    10561 cgatatccag gtcaccacct tccagacagc ccagcgatgt gatgatgtta agccagtacg
    10621 tcagcgggta tttgtaccag tgaacgtgat agaacgcccc gttcacctgc ttctcatcct
    10681 ttttcgccgt tccctgtgcc gttttaccaa aagccggcag gctgaagccc aggttcacca
    10741 tgcatcccgg tgaccgggtg acgtccgtca gcgccatcgg ctcccagtaa ccaatggcca
    10801 gcccgatacg cctgaacagc ggcggcggtg ccggacaaat ctgaatgggc atcgacgggt
    10861 tcgccgtgtc ggggacctta ccctgactga ctttgatact gcccagcgag agcgggaaaa
    10921 tacagctcca gcagatatcc gtgatcgggt ttacaaaacg cccctcacag gcagaatccg
    10981 cagatgccgc agggacatga ccggcgagaa ggaataacat cagcagccac aggcttcgct
    11041 tcattttctg ccctcctctg ccggaataaa ttccaccttc aggaagcgat cgccgggaac
    11101 ggcgctcacc cgtgccggga cctgatcaat gccgaggcgc tggcagagca caccgttctg
    11161 gtcaaagtaa acacggctgt ccagagactt ctgcatctcc gggatactgc cctgcacgag
    11221 gataattttg ctctccagtg tgggcggcgt ctggcgtttc atccaggcca cctgcgccgg
    11281 atcatcgcca ttgatgaaat acagcgtctg gttaaaaggc acatactgca gcggattcat
    11341 cacttcaccc tgacgggcaa acacccgccc ttcgttatcc cggatatccg cagccagtct
    11401 gacggacggg tcaaacaacc ggctgctgta tttctccgtg cgtccgatac cgggcacggc
    11461 agggggccgc agactgttgc ggatcacccg ctccttaaag gcatccattt tccggcccat
    11521 ctcaccggac tgctccagtg ccgtcaggcg ctgcatgatc accgtcagca tgtccggctc
    11581 ctttaccggc cacagatcgc cccaggtgcc aagatcggcg gcggccacac tctgccccca
    11641 tatcagcagg gcaatcagcc cccggcatct catggttccg ctctcattcg ccgggcaata
    11701 tcctgctgga tttcccgggt gatatccgga gccccctgta ccacggcagg cgacaccagg
    11761 atgaccgcgt ggtgtttctg ctgccacgcc tgcaggctgg cttccagtgc cgtgttaaaa
    11821 cgcgctgaaa gggcttttga ctgtgcttct gacagttgtt tctgactggc gctgtcaaaa
    11881 aacgcatcca cggtctgttt catgttgaag gcggcagtca ccggtgcgtt cagtctgaca
    11941 atgccgtaac tgacggctgc gttcagtaac accattgcca ggcatcccgg caccgtccac
    12001 caccagtggc tgcgtcgggg ggctgtaaca tccgtcgttt tttgcgttgt ggtcatgcaa
    12061 cactcctgta tttctcatgt tcttccagcc aggcttccag cgaagccatc tccggccctg
    12121 acttcttcca cgccagctgc cacaccgcct catgaatact cagcccctct ttacgtttct
    12181 gctgcacaaa ctcaaaatcc gggccgtcag agctgtacat ggcgcggctt aacgggtcca
    12241 caaacaggcg gtgccaggag gaatggtttt ccacctgcag caggaaggaa ctgaaccact
    12301 ggtctttggc cgcaccaaac ttaccaatca tgtcgcgctg cagtggctgg aactggtccg
    12361 gatacagctg gttgtatttt gcgaactcct tcgcactctg tttgaggata attttgtagg
    12421 aggagttacc ccatgccgca cgggccgcac tggaggcctt gtcagagtca aagtcgacga
    12481 tgttctgtgt gatggtgata taggcaccgg tatgacggcg ggcggtacgg taacctttct
    12541 caataaactc accgaccttg cgatttttga agtccagcag acgccagcct tcatcaataa
    12601 cgttcagttt cttgaggtta cgcggcgtgc ggtacatcct gttctcgatg tagataatca
    12661 gggagaacat caccgcaacc agcagtgacg gacggtcttc cagtccgccc agctccagca
    12721 ccaccatttt cgcatcatcc cgcagggacg gctcatcgga gttaaaatac tggccgtaag
    12781 tgccgttggc agtgtactgg tcaagcagca caatcatttc gtccagacgg ctgcggatgg
    12841 tcggtgaccc ggcatactgc tcgctgtcac tggcgttttt caggaaatcc accacgtcat
    12901 caatacgtgc cctgttctct ttggccagcc aggaggccct gaccgcctgc agcagcagac
    12961 cttcatgcac ttcatccagg ttaccgttgg ggctggccat cactgacaac tggtcacgga
    13021 cacgctccgc tgactggtca atatcggtga tgttcgcaaa cgggttaaaa cgcagggttt
    13081 caccgtccag atatacgcca cccatgttct cacacaggga cttgtagcca tcccccatgt
    13141 cgaacaccac ggcaaagccc ccggagtcca gcacgctgcg gataagtggc tgtatcagcc
    13201 cggttttacc ggccccggag gtgccacaga ccgccatgtt gtagttggtg ttattcatcc
    13261 cccggaagaa aatatcgata aacgccagct ggttacggta ggtgggtgcc agcagacctg
    13321 ccggtgtcag ggggttatcc gccactaacg gcatcaggtt ggcaacatta aagctctctg
    13381 cgcgctgaac cactccggcc tctttcagct gtttaaacaa ccctttcccg gccataaagg
    13441 gcagacaggt caggaaattg cgcatgtggt taaagcgcgg tgaaatcagc tcaaaaccgt
    13501 ttttacggaa gctgttcagg atgtcctgct ccacttccag cgccgtttca ttgttgtcct
    13561 tgcagaatgc tgtgatgttg aggaagtagg acacgacaga agactgaccg gagcccagac
    13621 gctgacgcag ttccccccac tccttcgcct ctttctccac ggacggaaac catttggcat
    13681 aggaggtctt cgactttttc tccaggtcca tgtacttcag gttggcttcg ctgtgggttt
    13741 tcacctggtc ttccaccacc agagtcagcg tcaggatgaa cggacaggag atggacagtt
    13801 ccgggttcag caggttgctg tagttgtcgg ccatattcca caggaaggcg atttccgggt
    13861 tacgggccag atggaaattc aggatacggg ccgtgctgtt cctgccgttc tcacgcaggc
    13921 ccagcgtcag gtaatcagcc cggacattca ggtcaaaact gtcctccaca cactgataat
    13981 tcagatcaga atacgggtcc agctgacgtc ttttcgggta cagggagtcc gggttatggt
    14041 taatcatctc cccgacaata tcgataaagg cctgtgcatc caccgtctgt gtggcgatac
    14101 tggccccctg taacgacgcc cggatgattt tcaccaggtt ttccatttcc agaatgtcag
    14161 cccggctttt tttcttcgag ggagaacagt acgagataaa tacccggtaa tggcgcaggg
    14221 tcaggggcag attcatcccc tccggcagcg gaaactgtgt cgctgccgct ttcatataat
    14281 aggcccgggt aatggcgtta aaccgttcag cctgttcacc tgaccaggag aactcacgca
    14341 gcccgtactc aatcctgtca ccaaccagct gactggacat cagatggata cacaacggaa
    14401 taccgcgcgg cagtttggtg cgcagcatgt gatcgagtgc ctccacaatg gacttattcg
    14461 ccccattgat gggaatggct tccagcataa agcccatcgt ggtgtcattc atgaacagac
    14521 cggattcctg gttgtaatca cggtacggca gcagacggct gaactgcggg aagctcattt
    14581 cgcccagaac ttcattggcc tttgcggatt cgtcaggcag tttcagtgct gtgacgaggg
    14641 agttaaccgc ctgagtgacg gcctcaagtg ggttattact catatgagta ttccttagtg
    14701 cgtttaaaaa aggaaaatat cagtctgtgg ccgtggatat atcacgcagt gccattcctg
    14761 aaaccacagc acaaataaga gatggaggct tttaacagga aaccaggatt aacgttcctt
    14821 tactgaatga gcatcattta atgcctgaat ttgtttttcg cgaatcttat tgcgctcatt
    14881 ttctcttaca cgtttcacct ctgccatttc gtttcttttt ctgcattctg atgcgatttt
    14941 atttgcctta ccaagtgtta aaggttcaga ctgataatta agtgtctcct gaaatgcttc
    15001 tgctgcacag ggagtttcct gtaaaatctc tgtcagttgt tctggtgttg caggttgttg
    15061 cgcaaggtaa acgatgtaaa aaggaataca tacaatcata gccataacgc caacaaatat
    15121 aaaagccaga acagatttta aatatatcga cttcatttca ttttccttcg aagcagtaag
    15181 cactcattgc ccaccaccat ttagtgtttc catcacgctg ttcaaaggtt catttgtttt
    15241 aacttcaata cactcccctg tatttccaaa gcccctgccg ccatgtgctt cgattgtccg
    15301 aagaacctgc acgcatacgt ccgtatttgg catgggtgta atattccagc ttccgccacc
    15361 gttaatattt tttatcagca caagaaaaat aaaggtatgc attctttatt atctccgctt
    15421 gtatattaat cactgaatat cccttcagtt aacttatgag gtcataaaac actgcagatt
    15481 ctcattcgga tttattccct tcagtaaact tccgaatagt atcctgttca cgctgacggg
    15541 tggcagactc ctgcattgca tccactttct cctgccagag acgccggata tcctgttcat
    15601 cccgaccagt cagtttttta ccggacaaca aacggttaag cagttcctgt ctcacatctg
    15661 gtacgtcttc agtcagacgc ataagccgga gtaattcctg acgactgagt accatgcgtg
    15721 attcctccgg tacgctgtaa taattaaaaa tacgactgaa taccccacaa cagcaggcaa
    15781 cggacaggca cagggatttc agatacaggc cagaaacaca gaatgtggca acaccacaaa
    15841 gaatagctat aactaaaaat cccttaccca gtgccataca caaagaaccc tgttttctgg
    15901 atatccgctc atgctcatcc agaataaaat ctattttttc attttctgat ttatgcatag
    15961 tgtttcctct gcctttcctg atacgcctga cattcaacgc acaacgtcac accgggaaat
    16021 attttccgcc gggcttccgg aataggattt ccgcatgctt cacagagata aacaggaata
    16081 ccatgaacat ttatttcctg gcgtatccgg tttattcctg tcatggtcag ttgttctgtc
    16141 actgaatatg cttcatcggc ttcatcactc acggaatatt cctcccgaca tgttctgaca
    16201 cgctaccgcc catccctttt cacgcacagg gcactcgttt tacaggcgga ttttgaatac
    16261 agaaagcccg ccggcattgc cggctgatat acaacttcag ggccagttaa ttaatacgtg
    16321 gttttcccca cgcagacggt ttaataacaa aaaacacgct ggatggctga tgataaacat
    16381 cctggttatc aatataaggc gctatccata atgctgccgt ctgctcaccc gttcttagcg
    16441 gacgaggagg tgttaccggc gtaactgaac tgaccggaac aaccgttttc acttcccggg
    16501 cagcggtaaa cagcgggcgt ggtgccagca gtttctgctc cggatgtgct gtcaccgcag
    16561 gtctgctgcc ggacggagtg gtcgcggtga cggtctgcac cggcattgtc cggaagttcc
    16621 cttcagccag gcgcggcagt gatgccgcaa ccggctttgc ttctgaggac tgctccagtt
    16681 ttttggcctt ctcattggcc tgctccatcg tcatacaggt atcggatgtg gtggcgttac
    16741 actcaaattc cgtatttgtt cctgcgcagc cggaaagtaa taaggttccc agcagaggaa
    16801 taaataaaga aatctgtttc attgcccttc tcctttttta ttactcccca caacagcaga
    16861 aattaactct gctgtcagat ttgttttccc tgcacaggga tgtccggaga ccactgtatt
    16921 tcccggagaa gaaaataagc tcatcgacat tcccttcttc atcacaccga cagcagccat
    16981 attaaaattg tgcggcaatg attcagaact gaacaccttc ggagcaggaa gaactatttt
    17041 tgtggtgcag ttactgagaa cagcatgcat aaaatcatct gatacgtttt tatccggcac
    17101 aacgcgggct gtaataacat taatattacg catgttcatt tttatctccg ttgcttctgt
    17161 ttgttcggtc atcagggaaa taaacatggc atttcctgat ttttcccggc agggaagcgt
    17221 cttccgtata aacgtccttc agatattcat ctgcggttac cacccggata catttcattt
    17281 ttccgggcag caccggatat ttcccggtgc ctgccgcaaa cgacatatac atcagtgcca
    17341 gcgttgccca gaatgcggtg accagttgca tcagtgaccg gtcagtctcc ctgacataat
    17401 ctgcagcatc agccgcgcgg taatggcaga tatcctgtat ttctgatctg cctgaagaat
    17461 cagaggaaga aaaccagcat tcactgaccg ggaactgtcc ggcaggtgct gtttgtctta
    17521 cgaagttaat ctccttaacc agtaatttcc ccggcgtggt gttgtccttc cataaaacaa
    17581 acagagccat cagggccatt gtggccagtg gcagaatgac ggtgtacttc agacaccaga
    17641 acagtcctcg cagaacccgg gcgacaacat accgggcggc atggcacgcc tgacgggacg
    17701 acatattatt tgccatcgtt gccccctccc tggctgccgg acataaactg ctgcggattc
    17761 aggttgccca gctgcttcag catctggtca gtattaaagc cgttaagatg actttcagcc
    17821 gatggcggaa caggaaccgg actttccgga ttgtcttctt ccgctctgct ctgcgtgcgt
    17881 tccagcgcca tctcttccac ggttttcagc tggaagccgt cctggaacac cacggtcact
    17941 tcgttgcccg caccaatcgg tatcaccggg tgatactgtt cggcacgttt gatgtagtag
    18001 tcactgagcg tctgtgcggc tttcgatgcg ccgccaccga tacccatttt caggacatca
    18061 ccagccccgt aagcggctgt ggcacccagc ccgacagccg gttgggaggc gcgctccatt
    18121 ccctgaccga taccgtcaac aaatcccgcg ccccatgccc agccgaggat tttgccgtta
    18181 cgcatcacca cttcgccctt gataccgttt ttaccccgga agctgacatg ccccttaatc
    18241 ggcatatcaa tagttttgcc gtccttcagg caactgatat tgcgggtgcg tacaatggca
    18301 cgttcactgg acacatcccc ccaggcttcc agccccacaa aacatcccgt tgcgtcatac
    18361 gtcttgctgt tcggcatttc caccaggccg gtgatacgca actgcatcgg caccgtggat
    18421 tcattaccgg tgactgaggc attggcatcc gccccttcaa tcagcatggc tttcgcaaaa
    18481 cttcctgacg gaatgtacgg cagcgatggt ccctgttttc cctcattacg ggtaaacacc
    18541 ttacgctgta tccggttagg caccggcaca gactggtacg tcacctgtgg tggtggcgtg
    18601 acaccattcc cgggataaaa cgccgtcggc ggtggaacag caacgctgcc ctgcggcggg
    18661 aaggatacgg gggtgtttcc tggctgtggt tcgccttccg ggccgggcgg tggggcaggc
    18721 atctgcggta caggctcacc ggtcgccgtg acgggattag cacccagggc ttttacctgc
    18781 tctgccaggg cggcattgtc ctgtcccagc ttttcaatac gacgctggtc atcaccgcgc
    18841 tgtttgttca gaacatccag ctcacgacgg atttcctcat actgcttctg catctgcgct
    18901 gccgtcacct gcatctctgt ggtggcatgc tgacgcacct tgtcatcaaa ggtcgtatcc
    18961 accacaccgg tcatatccgg cacaggctcc tgttcagcca cggtttcacc gttaccggac
    19021 atgtccacat cagacagata cagtgcccca ccaatcgcgg aggctgtacc gacaaccaca
    19081 atccccagcc acaggtactg cttgcgtttc acaatggtat tgatactggc catcagttgc
    19141 cctccccgtc acggatgaca tacacatcca tgcgcgcacc tgccagtaac tggctggcag
    19201 gctggctgaa catcacggca cgggttcccg gctgccagaa atcactttcc cggatattca
    19261 gggcagacag cgttttattt tccacgacaa agcggaccat cttcaggtga ttccccgtcc
    19321 atacggaatc tgccactgaa gacagccccg ccggggcctg cagggtttcc tttgtcacag
    19381 gaacctgata ccagcctgca ggtaattttc cgccacggac agcctggctg atggtcacaa
    19441 gcagggattc gtaaggcgtg gacgtttccc acgctccggc ttcttctccg gttccgcgca
    19501 ggtcactgac cagctgaatg gtacgccccg cgccttcacg gggaacagcc tgaatggaaa
    19561 ggttcagacc acgctctgtt tccagaatga acgtaaaggg ctttttgttg acggtggcca
    19621 ccaccacacc gccggaggcg gtctgctcat tattggtcag ggcaccatcc agactgttca
    19681 cggcgataat ccggtcaccg ggaacggtaa acagattcgg gctggtattg ctgatggcaa
    19741 tactggcctg accaccgttc accattggca ccacagtggg ggccagcgta ccgtttgcgg
    19801 ccatcacgct gcaggaaaaa aacaggccgc cgaatattat tgccgtatta ttttttctca
    19861 tcgtctgttt ccccgaaatt atccagccag gtcaccccgt tttcccgctt cagaataagg
    19921 atgtaatgtt tgatatcagt aaaaggttcg gagttaccaa tccatgtttt ccgaattcca
    19981 cggatttcca cacgaccata ctgaggccag acacggacac tggtctggaa aaaagcagaa
    20041 ttcacgttat cttttttaat gagtttcgct tcttcagcca gaataacttt catctggttc
    20101 tgtgcacccg ggcggatata ttgcagaagc gcctgatgtg aagcatcgac gatttccggt
    20161 gagacattca gacggagggc aataaatgac agcgccatct gctgcaaata agaggcatcg
    20221 gcactgttct gtgacacggc aaagggcaca ttaaatccca ttggggtcac ggcagtgcgt
    20281 tgctcattct gaagacggta gttattcacc ccctgaatga ggttaacaga gaggctgaga
    20341 acaataagca ctgacataaa tataaaggcg atggccatta cacgactggt acttaaacgg
    20401 gcaccgtgtt ccatataaca atcctggtat cagttctatt taatccactg ccggaaacac
    20461 gaatcgggaa cattatgaaa aataccgcgc agcagggctg ttggcatata ccagtaaatc
    20521 aggtcacgta accaggaact gccccgccct tttttcagtt ttttaatccc gaaataaacc
    20581 agaaccgctg caccaatacc gaacagatat ttcgatgttg tgatacccca gccaacacag
    20641 attgctgcgg ggatcagttc atccagtggc aggccaaacc agcggctctg gttggtcagt
    20701 gtttccggga aacgatattt c
//
LOCUS       DAEEKQ010000063        20711 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.063, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000063 DAEEKQ010000000
VERSION     DAEEKQ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.063"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(73..4533)
                     /gene="mukB"
                     /locus_tag="KLK57_002204"
     CDS             complement(73..4533)
                     /gene="mukB"
                     /locus_tag="KLK57_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HBE0892567.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTAQRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     EQEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNVVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQES"
     gene            complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="KLK57_002205"
     CDS             complement(4533..5237)
                     /gene="mukE"
                     /locus_tag="KLK57_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HBE0892568.1"
                     /translation="MSSTNIEQVMSVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="KLK57_002206"
     CDS             complement(5218..6540)
                     /gene="mukF"
                     /locus_tag="KLK57_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HBE0892569.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="KLK57_002207"
     CDS             complement(6537..7322)
                     /gene="cmoM"
                     /locus_tag="KLK57_002207"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HBE0892570.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7458..8237
                     /gene="elyC"
                     /locus_tag="KLK57_002208"
     CDS             7458..8237
                     /gene="elyC"
                     /locus_tag="KLK57_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HBE0892571.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTAKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEELNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8214..9107)
                     /locus_tag="KLK57_002209"
     CDS             complement(8214..9107)
                     /locus_tag="KLK57_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HBE0892572.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="KLK57_002210"
     CDS             complement(9261..10007)
                     /gene="kdsB"
                     /locus_tag="KLK57_002210"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HBE0892573.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="KLK57_002211"
     CDS             complement(10004..10186)
                     /gene="ycaR"
                     /locus_tag="KLK57_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HBE0892574.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10238..11470)
                     /locus_tag="KLK57_002212"
     CDS             complement(10238..11470)
                     /locus_tag="KLK57_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HBE0892575.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="KLK57_002213"
     CDS             complement(11507..12493)
                     /gene="lpxK"
                     /locus_tag="KLK57_002213"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HBE0892576.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="KLK57_002214"
     CDS             complement(12490..14238)
                     /gene="msbA"
                     /locus_tag="KLK57_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HBE0892577.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAIDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14275..16539)
                     /locus_tag="KLK57_002215"
     CDS             complement(14275..16539)
                     /locus_tag="KLK57_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HBE0892578.1"
                     /translation="MKITTVDVCIICGIFPLLILPQLPGTLALAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITYLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQLWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASLIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAVLLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSSKVKHRYQQQGYKWLDTPHQGQIAVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="KLK57_002216"
     CDS             complement(16746..17030)
                     /gene="ihfB"
                     /locus_tag="KLK57_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HBE0892579.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="KLK57_002217"
     CDS             complement(17190..18863)
                     /gene="rpsA"
                     /locus_tag="KLK57_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HBE0892580.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="KLK57_002218"
     CDS             complement(18974..19657)
                     /gene="cmk"
                     /locus_tag="KLK57_002218"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HBE0892581.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPASDALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="KLK57_002219"
     CDS             complement(19830..20594)
                     /gene="ycaL"
                     /locus_tag="KLK57_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HBE0892582.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4970 a         5732 c         5045 g         4964 t
ORIGIN      
        1 aatgtgccac ttttttagtg cagaaacgga gttttcggaa aaagaaaagg cggcattgct
       61 gccgccttaa ttttaactct cctgagaagg cgcttcgtca cttcctggaa gcgtttcagg
      121 gagttgcggc gcaaatcctc gcaggccgac gacgtgaacg tgttcggtat tctggaagac
      181 tttacgcacc agtttatagg tggtgccttt ctccgggctg atattttccg gcgctgcgat
      241 gatgagctgc atttgcaaac gttcacacaa ttcaaacagc gtggcgatag aacgagcatc
      301 cagtcgcgct gcttcatcga ggaacagcag gcggcaagga gagatatctt taccgcgcag
      361 gcggcgagat tcatcttccc agctttgtac caccatcacc agaatcgaca taccggtacc
      421 aatcgcctca ccggtcgaca atgcaccaga ctctgcgcgc aaccagccat cggaaccacg
      481 gttaacctca acttccattt ccagatagtt gcggtaatcc agcagttctt cgccaatggt
      541 ctgcggcgtg cgctgcccca tatcaatctg cggattaaga cgctgataca atttcgccag
      601 cgcttccgag aaggtcagac ggttgctgtt aaacagatcc tgatgctgct cgtgctgttc
      661 agagagcaca tccagcagca tggcgtgcgt ttcacgcacg ttcacgttca gacgtacgct
      721 gttcacctga ccaaacgata cgttctgcaa cccctggttg agcatacgga tacggttctg
      781 ttcgcgctga atggttttgc gaatgatgtt cgccacgctg cgggaactga tcgccagttt
      841 ctgttcgcgg gaggttaact cctcggtcag tcggctaagt tcgatttcca tctgctcgat
      901 ggcttccacc ggatcatcgg tacgaataat atcctgacga atacgttcac gcagatgctg
      961 ataaaccgcc acgaagaact gaattttacg ttccggacgt tttggatctt ccgacatgcg
     1021 cagcacgtcg cgcagatgtt cgttatccgc caccgccaga cgcagcgcac ccaatgcctt
     1081 atccgacata gagcgcaaat catcagcgga gagataagcc agctcgcgac ggtgtaagcg
     1141 ccgttcaacg acgttatctt tcaccatgcg catcactgca caccagcccg ctttggcggt
     1201 cactacctgc tcgcgcatct caaagtaatc ccgctccagc ttgcgcagtt tgcgggtcag
     1261 attgtccatc tccgcttcgc agaaagtcag tgctttttcc agctggttgc ggcgggaccg
     1321 gttattactc agttgcgcat gcagctcgtc acggcgaatt cgcgcccgct cttctgcccc
     1381 gctatcggcg cgcacgccga tatcctgcaa ttcacgttgc agatcgttga gtagctcttt
     1441 tttggtgtcg taagaacttt tcagcgaagc cagcacctgg ttgtactgac tcaactgcgc
     1501 tgcgtgacca cgcaacgctt cacgagcgcg agtacgctcc gcttccgcct gttccagacg
     1561 ttcacgcagc ttttcgttga gatcgctatt accgctcagc atttctgccg agtcagaata
     1621 gctaaagtgc gcacgacgct gcaccacttc cgtcagggca aacgcctgct gacgggcatc
     1681 gcgctgcatc tgctgagagt acgcgtaatc ttcttttagc tgttcgaact gttccgggtc
     1741 gctctgcaat accgaaacga tcggttccag tttcgccagt tgattgccaa actgctgaac
     1801 aaaacgcgcg gcttcctggg cttcatccag acgttcgcgg atttcatcga cgcgatccgc
     1861 caggctgtca tcagccaaca ggttgagacg cggcagaatg cggttcagcg ccgtaacgcc
     1921 ttctttcgcc tgctcaaact gaatacgctg ctgctggtta tcattttcat gattactcaa
     1981 cgcccgctcc agttcgacgc gacggctgtt cagttgacgg atttctgctt ccgggtcaga
     2041 ctcaaacgcc accgccagat gactgccgat aaagcggctg aacgcctgat gcagacgctg
     2101 ggttttctgt acatcaaagg agagcgtggc gaagcgttcg gaaagcactt cacgctcggc
     2161 atggaggctt tcaatacggc tttcacgcgc agcacgacca aacagcggca cttccgggaa
     2221 acgtgaataa cgccactgac gatcggcgat tttcaccact accgcttttt ccagctcatc
     2281 aacgctgaac acgctgtcat cgaatgactg cggatcccct tcgatcaaat agagatcttc
     2341 cgggcaatcg gtcaagcctt ccaggtgttc agttacctgt gacagatctg gcaccacgat
     2401 ggcgtggcgt gacgggccat acagcgctga gaagtacggc gcatcttcca ggctaacgtc
     2461 gtcataaatt tctgacagca gcacgccgcc aaaacgctcc gccagcgcgt tcagacgctg
     2521 atcttcagag ccgccaggct ggcttaaacg ttcgatctct tcatcgacag cgtttttgcg
     2581 cgcgcccact tcatcgcgtt caacaatcgc ctctcgctca cgctccagca actgttgcag
     2641 atattcggtg acgtcctggc tggaggtaaa ctcttcaccg cactgctcgc tcaactggtt
     2701 gagactgttt tgcgctgcca gccaaaccgg cgcacgctgc atcaaactct gaatgcgaga
     2761 ctgcagctgt tcctgttcct ggcgcagtgc catgcgctct tcacgggcgt tagacacgct
     2821 atcggaaaga gaggcaatgc gcgcttccag ctcctgatgc agagcttcca gttcatcaat
     2881 atcaaaattc ttgccctgac gtttgcagaa gtctgccagc aggcgttcgg cttcttgctg
     2941 ctcgcgcaga cgctgttcca gttcacttaa gcgcatccgc aacggttgaa cctgctctgc
     3001 caggtgacgc tgatcgaccc cttcgcgcag cagttcgcgg gcgacatccc aagcctcgtt
     3061 acgcgccagc gggccgttga ttgccaccac cagctgataa gcctgctcaa actggctgtg
     3121 cgcggtttgc gccatgctca ttttctgctc aagagagagc attttttcag tcgcttcctg
     3181 ctctttcgcc tggaaggttt ccagccattc ggcggcgctg tcggcggtta agtccggcag
     3241 atggcacagt tctttggcgc gattaagcgc agcaatcgcc tggttatact ggatcgcgcg
     3301 cgtttgctgg acgtccagcg cctgctggta gtcagcaagc tggcttttca gctcgtccac
     3361 ttccagttcg gcagcttccg cgcgagcctc gttctcttcc tggcgttcga tggcttctgc
     3421 caccacttcg ttttgctctt ccagacggat ctgcagctca tcgagatccg cttcgtagcg
     3481 ttcgatcttc tcctgctgac gctgtgcggt ttgcaccagg ttcaggtgat cgctggccgc
     3541 ctgataatcc gcttccagat cgccttcggc accgttgtgc tctgccagct cacgcgccat
     3601 atcgacgtgt ttgtactgct cagccgccag ttgctgacgc gaagtatgta actcgcgacg
     3661 aaactccagg gctttgtcga gatggacacg gcgctcgttg gcgtgacgca tgtagtccgc
     3721 cgccacgtag ttggtagctt cgctgatcag atgcttaaac aggtcacggt ccgactgggt
     3781 gacacgaatc gcttccagcg tcatgcggtt ttcacgcagc gccgcttcca tgtcctggaa
     3841 cgctttacgc acgccgctgt tttccggcaa caggtagtcg cgcagagaac gggtaatggc
     3901 actggagatc ccgccataca gcgaagcttc gatcagacga tagaatttgc tacggtcaga
     3961 tgcggagcgc agacgacgcg cgatgatgcc cagatcgaac atcagcgagt ggtaatcggt
     4021 aatggagttg aactgtttaa actgcacgcc ttccatcgcc tcgagcttgt ctttcagctc
     4081 gttaagcggc agcacgcgcg cctggcgttc gttcagggtt tcggtcacca gctgtgtcgg
     4141 ctgcaccgac atcggcagtc cctgaatggc aaatggtttg atatccactt tacgatcgcg
     4201 tccggcaacc tgttgcagac gcacaccgac caccacgcgc tggtggcgcg agttaatggt
     4261 gtcgagcatc gaataacaga cacccgcttt cagcttaccg tgcagacctt tatcgcgcga
     4321 accgctggtg gccccggctt ccgtagtgtt acggaaatgc agcagggtca ggtcggggat
     4381 cagcgccgta acgaacgccg ccatggtggt ggatttaccc gccccgttac cgccagaaag
     4441 cgtcgtgacc agctcgtcaa ggtcaaaagt tcgggcaaaa aagccgttcc agttaatcag
     4501 cgtcagtgag cgaaatttac cgcgttcaat cattattctt cctctccgct atctggctga
     4561 ttctcttcgg tttcatcgtt gagttgcaga tgattttcaa tcggcattgc ttcgccatca
     4621 cgaatcaggc gacgctgtgc ttcacgggga tcgtcgccag cacgcacatc ggctccgaag
     4681 cggaacaccg attcggtaat gcgaaacttg ctgctgtcgt ggcccataaa ccacaccatg
     4741 cctaaacgac gcagacggtt gagcgaagaa cgtactttct cctgcaactt ctgacggtca
     4801 acgtctgaac cggttgaacg gttgttcacc agtttcagca gttttgcttc atcggccagg
     4861 gtgagcagtt cgtcgtacag ttcctgctgg gtgaaaatcc cctcattcgc cagccgttcc
     4921 gggctgagat agagataaca gaggattttc ccgaccatca tatccagttc cgacaagacg
     4981 gaacgcggga taagagtggt ggaacgtggg cgtaaataga agaacccttc cggtgcgcga
     5041 ataagctcaa cgttataacg cgcgtaaaac tcttccagat attcctgaaa atccatcagg
     5101 aatgcatgat tatccagttc gtcgaggcca atatggcggc ctgaacgtaa ggcgctgtcc
     5161 agcgccggaa ataacggatt cgccagcgcc tgcgccagct taaccgacat cacttgttca
     5221 atatttgtcg atgacatgcg cctgtacctt ggctccgtaa tcattaatcg gctgccattt
     5281 cgctggcagt ccggtgaaat ctgcttgcgc tacgccaaga cgtaccgcct gatcaataac
     5341 aatacgcgca acgtcaaagt gacgtgcacg cggatactgt gacagatatt cgcgtaccac
     5401 cagaccaaga tccagcggca cttgtctggt tttgtacacg gcaagttgtt cttcgatgat
     5461 cgccgccagc tgttcgcgga tctcgttaaa ctcttcgtat tccagatcct caggaagttc
     5521 cccagtcact tcttcatcgc gcagtgccat ctcttcgtca cgcatatcca gcagacgatc
     5581 ggcattggca taagttagcg cccacggctc atcaaaatag gtttgtaccg actgacgtaa
     5641 ccgctgagca aagacgcggt ttttatccat atcgatcgcg gtacgaataa atttgtgtac
     5701 gtggcggtcg tagccaatcc acaagtcgat ggattgctgg ccccaactga taatacgatc
     5761 gagtttgctc tgcagatcga acaccagacg atcgacgaaa tgcagatcgt catgggtcat
     5821 cgtcgcatcc tgaatgcgca acagattagc ctgcaatttg tcgcctgccg cttccagcgt
     5881 atcctgcaat tcacgcagcg ttccggaagt ttccgaaagc aacaattcac agctggaaat
     5941 cgccgcccgc cagtctttgt tcagcaactg ggcgatatcg tccttcacct gctgctgctg
     6001 ttcgtccatc agacgttgcg tcaggtcgat actgtcgaaa atttctgcta ccgaatattt
     6061 cagtggcgca tagacattac ggtgccagtg aaattcatca ccgccctctt cggcggcatc
     6121 tgctgcgcgt ttgagctcac ccgccacaat cgacaactgc atagaaagac gcagcgtaga
     6181 aaactcgcgc tgacggatgt agtagtcagt aatgccgatg ccgagcggcg tcagacggta
     6241 aattgcgttc ccttccgcct gctcgctggt aaagcggttc agcagacgtt gacgcaccat
     6301 gtcgttgatc gcgttattgg cgcgcacgcc gatggtttcg ctggtttgct caaacgcatc
     6361 actcacatgg cggaatgcat ccaccagctc gccttcactc atctcaccat ccagacgctc
     6421 gccgttcagc gtggcaaccg ccagcagaaa agagagtcgg tctaccggca gcgagatgga
     6481 gaagtcattt tttctggccc aggcaaccag ttcggggact gtctgggaaa attcactcat
     6541 actttatcct tgctctgcgg tttgcgcgcg gtgacatgaa tataacgccc cagggtaata
     6601 tacggttcct gacggcaata acgcgtttct aattcaagta atgcttcata gcagtcgcgc
     6661 tgctggtgct tctcgcgcag ataatcatga aacacgcgaa cgcctgtctt acccataatt
     6721 tgccaaccag cttcttccag ccacagataa acctgcgccg ggtcgcgtgg ataatctggc
     6781 gaaagcgtcc gttttttctt tttcggcatt cccgcctgca cgtaatcaaa attcccggcg
     6841 accatgttat gcatcaacaa accatgcgca ttgtagaaca ttaacgacaa cacgccgcct
     6901 ggacgcaaca ctgaccacag ggtctgcaat acactgcggg gatcggccac ccactcgagc
     6961 accgcatgga acaatatcag atcaacgggc gtttccaaat gcgaagcaac atcctgagcg
     7021 gcgcaatgta taaattgcat gttgtcgctc acaccttttg cttctgccgc ctgttttgcg
     7081 cggtcgatca tctgcgcaga aagatcgcat aaaatgacct gatgcccgcg ctcggccatt
     7141 ttgattgcgg tctgcccttc tccaccgccc gcatccagca cacgcagttt ttgcggaccc
     7201 atttccgcca gcacgcgatc gagatcctgc cacagaatag cctgtcgaag ctgccctttg
     7261 gtggtgccgt aaatgttacg ggaaaacttt tccgcaatat catcaaaatt gcgatcctgc
     7321 attgctttct ccaccggttg gctaagacgt tttctgataa agacgctatt ttgtcacagc
     7381 cacggcgaga atgaacccgt ctggtgtaat atcgctggcg atcccttgct atatggttaa
     7441 aaaaggaacc agaaaggatg ctttttacac tgaaaaaagt gattggcaat atgttgctgc
     7501 cacttccgct gatgttgtta attattggcg ctggcctggc gctgctgtgg tttagccgtt
     7561 ttcaaaaaac cgcgaagatt tttattagca ttgggtggct ggcactcttg ttattgagtt
     7621 tgcaaccggt agccgaccgc ctgctgcgtc ccatcgaaag cacctatccg acatggaata
     7681 attcgcaaaa agtggactat atcgtggtgc tcggaggtgg ttatacctgg aacccgcagt
     7741 gggcaccgag ctctaattta atcaataaca gtctgccacg cctgaatgaa ggtattcgct
     7801 tatggcgcga aaatccggga tcaaaactga tcttcacggg aggcgtagca aaaaccaata
     7861 cggtgagtac agcggaagta ggtgccagag ttgcgcaatc gctgggcgtg ccgcgcgagc
     7921 aaattatcac cctggatttg ccaaaagata ccgaagaaga agctgcggca gtgaaacagg
     7981 cgattggtga cgcccctttc ctgctggtga cctccgcatc acatctgccg cgggcgatga
     8041 tcttttttca gcaggaagag ttaaatccgc taccagcccc ggcgaatcag ttggccatcg
     8101 actcgccgct taatccgtgg gaacgggcaa tcccttctcc ggtctggctg atgcatagcg
     8161 atcgtgttgg ctatgaaacg cttggccgca tctggcagtg gctgaaaggt ccgtcaggcg
     8221 agccacggca agagtgattt tgatgccaga tcgaagttgc gccgactaaa tcgtccggta
     8281 ttaaccagtt gcgcaacttc atcccataac acatacaacc aacgccgcca gatgaacgac
     8341 tccgccactg gcgcgcgttg caggtaactc cagagcaaat cttccgccag agaattatcc
     8401 attagtcgga acagttcgta ttctcgcggt gcccaaagca ttagtcccgg cccgaccatc
     8461 gccagtaact gatcgctgcg cgaatctttc agcatgctgc gtaaacagaa gttaccgtga
     8521 atcaacacac aattgtcgtt aaagccttca aataatgccg ggagacattc gcgagtgcga
     8581 aacaggatcc gcttatcctg catcgtcaga ccggtgttat tgaactgatt gagcgtggtc
     8641 catagcactt caacatgttg ccggtaccat gagggccaga aattttcctg agtattgtcg
     8701 accgcgccga cgcaaccgcg actgtcctga cggtgccagg ccagtaaggc ttcaacgatt
     8761 tggtctttga gctgttccca gcgttctggt gttcgggctg gtgcctccac cgaaacacca
     8821 cgcatccgct ccagtagcag gacatcaggg ccggggtgtt cttcatgcgt catcacacca
     8881 taaatagtcg gcatacggac agtcccactt cttgccagca tggtggtttt ccatgccagt
     8941 tgtcgggctt ttccgggcgt actaaagctt cttgccatta acggcattgg gtttccctgg
     9001 ctgtcataca aagcccacaa cgccgtatcc gctttttcat tgacgcactc aatacgactg
     9061 agtttttcgc ccagtaaatg gcttaattcg gcacgcagct gttccattca gattaccctc
     9121 ataaaagcta ctgctttcat aatgagcgcc aatataaaag atgtcacctg aatgagatca
     9181 attgttgaaa agaaaaattg aggaaaatcg taacggtacg acactcctcc caaaattggc
     9241 tgaagtgtcg tgaagtgaaa ttagcgcatt tcagcgcgaa cgcgctcaag atcttcaggg
     9301 gtatccacac ctgtgccagg aacttcctga gcaacagcaa catggatttt ttcgccgtac
     9361 cacagaacac gaagctgctc taacatttcg atgtgttcta acggacttgc ctgccagttg
     9421 acgtaacgac ggataaagcc tgcacggtag ccataaatac caagatgacg caggaagtta
     9481 tcgccaacgg tttcaaggcc ttctgcaaaa cgatcacgat cccaaggaat ggtggcgcgt
     9541 gagaagtaca gtgcataccc ttcagcgtcg agaaccactt tcaccgcatt cgggttaaac
     9601 gcttcttccg cattgtggat tggcaccgcc agagtcgcca tacccacctg acgctgagcg
     9661 aggttatcag caacctgacg aatgattgtc gcagggatca tcggttcatc accctgcaca
     9721 ttaacgatca ccgtgtcgtc gctgaatgcg catttttcga caacttccgc cagacgttct
     9781 gttcctgact gatgatcggc gcgcgtcata catacttcac cgccagcggc ttcaacggcg
     9841 cgggcaacat cctcatgatc ggttgccacg atgatgcgct cggcacctga ttcacgcgcg
     9901 cgttcaagaa catgaacaat catgggtttg ccgttaatat caaccaatgg tttaccgggc
     9961 agacgcgtgg acgcgtagcg cgcgggaata atgaccacaa aactcatgat ttactctcat
    10021 cagcagtcag cacgcgggct tccgtttcca gcaacactgg aatgccatcg cggagcggga
    10081 aagccaggtt atccagtttg caaataagct cttgtttttc ctggttatac caaagttttc
    10141 cgttgcaaac cgggcaggca atgatttcaa gcaaacgatg atccatagtt cctccagatg
    10201 gatcgggtta tgaatgcata aatcttatca taatcattta tgcgacgggg tctatttccc
    10261 agccagtgcg gcaatcagta aagagacctt gcgggcagcg tcctaatgtc acccgcgttg
    10321 cctgctgcca gctcgcgaaa tcagtaatcg cctgacgtaa ccctttttgc agcattgtcg
    10381 ttggtttaat gccttcctgt aaccacagag agataacttc aaggatgcct gtctggcgat
    10441 gcattttggc atccattcgc ccaactaatt gcccacgatg taataatggc agaacaaaat
    10501 agccatactg gcgtttcggc gctggggtat agcactccag ccggtagcta aaatcaaaaa
    10561 gctgctctgc gcgtttgcga tcccagacaa caggatcaaa aggcgaaagt accgcgctgt
    10621 gcgttgcagt gagctttccg gccagcgcac gctcgagtag cggcagcaaa tcagcatgca
    10681 gccaaagatt gcccaatttt tcaacatgca cagcaatgat ttgctgctgt tcagccctcg
    10741 cttcgcgcca cgccgccagt gccggacgtt tcagccgata gtaatctgcc agccactgtt
    10801 cgcggaatat tcccaagcta cgcgcactgt tatccagcat gatgatttct gcttctgttt
    10861 gcgaaacgag atcgcgctca tcatcccagt caggcatgac acggtgggtt aaatcataaa
    10921 cgcgctggaa gttgcgccgt tcaatcacca tcacctttcc ggcagtaaat aaaccttcca
    10981 gatgccgttt atgcggcttc cattcccacc agccgcttgc acctttacga ggatgctcaa
    11041 aatcggctga acgtaccggc cccttatcat gaatatgctg aattaactgt gcaatttccg
    11101 cctcatgttc ctgcatccag gcgtctttgt atttccagcc cattttttca ggtgccagca
    11161 tgcggtggcg aataagacga aagtcgctac gcggcataaa gcaggcttca tgcgcccagt
    11221 attccattaa ttcgccacgc gccagagact catccagcca ctgggcagga taatttccca
    11281 gacgactgaa aagcaccaga tatggactac gggcaacaat attgatggta tcgatttgca
    11341 gcaaggacat gcgggagatc gttgccggaa tatcctccaa cgacgctcga cggcggggtt
    11401 tgtttaacag gccttgtgcg gcaaggtgaa gattacgcgc atcagcaagg gagaggtgcg
    11461 gcagtgacat tcatgactcc atcaatcgaa cgctgccgcg gcgtaactag ttgccagaag
    11521 ccagcgaggt tagttgcgta agcagtttcg ctggttcatc acctgaaagc tgtgcgtcta
    11581 caggcaaata ccaccaattt tcttctgcaa aggcccggca tttcaccgca tctttttcag
    11641 tcattaccag cgtttgcccg gcgcttacca acgcactgac atccgcatgg ttcaaagact
    11701 gatgatcggc cagcggtaca catttttccg gttgtacgcc acacatcttc agcgtggcaa
    11761 aaaagcgcgg cggatgccca atccccgcca tcgccactac atgttcaagc tgagcaacgt
    11821 cacaacgcgt accggtacgt aaattcaccg cctgacccgg cagcagatgc atggggattt
    11881 caccgctgcg agggacaccg ccgttgacga ttaccgcatc aaccgacttt aagcgccccg
    11941 ctcgctcacg cattggcccc gccggcaacc accagccatt gccaaagcga cgcacaccat
    12001 caataacgac aatttccaca tcacgcgcca gacggtaatg ctgtaaaccg tcgtcggtta
    12061 cgatgatctg cacatcaggg tgttgcgcca gaatggcttt taccgcatca gaacgaacgg
    12121 gagaaaccgc aacaggcgca tcagtgcgtt gataaatcaa cacaggttca tcacccgcct
    12181 gtgctgttgt ggtatctgcc gacaataaca gcggataaga ttcagcctta ccaccatatc
    12241 cccgcgatac gacccccacg cgaataccgc gctgttgcaa ctgttccacc agccagacaa
    12301 cgaccggggt ttttccgttg ccgcctgcgg tgagattacc aaccacgaca accggtacgg
    12361 gggcacgcca ggcgcgcttc agttttagtt tatagcaaag acggatcgcg ccactcacca
    12421 ggccatacaa ccaggagagt ggcagcaata gccgccacaa aggggattca ccagaccaga
    12481 ttttttcgat cattggccaa actgcatttt gtgaagttgc gcgtaaacgc cgcggtgctc
    12541 aagcaaatcg ttatgcgtac cgcgttccac aatgacacca tcctcaacga ccacgatttc
    12601 gtctgccttt tcaatggtag acaagcggtg ggcaatcacc agagaggtac ggtttttctg
    12661 caactcatcc agtgccgcct gaatcgcacg ttcggattcg gtatccaacg ccgaggtagc
    12721 ttcgtccaga atcagaatcg ggctatcacg caacaaggct cgagcgatag caatacgctg
    12781 acgctgaccg ccagagagca gcacgccgtt ttcaccaatc actgtatcga gaccgttatc
    12841 catcttattg atgaagtcta tggcgtaggc catacgcgcc gcttcttcaa tttgctcacg
    12901 gctgtactgt tcagtccgtg cgtaagcaat gttgttagca accgtatcgt taaacagatg
    12961 gacattctgc gacaccagag caacctggtt acgtaacgac gccagggtat actcgcgcag
    13021 atcgtgacca tccatcagga tttcgccttc atcaatatcg taaaaacgcg tgatcaggct
    13081 ggcgatggtt gatttacctg aaccagagcg tccaaccaga gcgaccgttt tccctgccgg
    13141 aattttcagg ttgatgttac gcaatgcggg cacgtcacgt cccggataag taaaggtgac
    13201 attgcggaat tccacgtcgc cagtcgcacg ctcgatcacg cgcttacctt cgtctttctc
    13261 ctgctcactg tccagaatgg taaacagcgt ctgacaagcc gccataccgc gctggaactg
    13321 ggcgttaacg ttggtcagcg atttcagcgg acgcatcagt gcaatcattg aagagaaaac
    13381 aacggtaatc gtaccggcag tcaggttatc cataacactt gggaagctcg ccgcatacag
    13441 aacaaacgcc agcgccaaag aggcgattag ctgaatgatc ggatcagaga tggaagaggc
    13501 tgaaaccatt ttcatcccct gaagacgcat tcggttactg actttatcga agcgtttcgt
    13561 ttccacttcc tgaccaccga aaatcaatac ttctttgtgg cccttcagca tttgttctgc
    13621 gctggtggtc acctgcccca tggtgttctg catgttttta ctgatgttgc gaaaacgctt
    13681 cgatacaacg cgaatcgcaa tcgaaacaat cggtgccagc acaatcaaaa tgatcgacag
    13741 ttgccaactg taatagaaca tcatgatgaa caggccgatg attgacgcac cttcacgcac
    13801 aaccgttatc agtgcgccgg aagaagaaga agcaacctgt tcggaatcgt aggtgatacg
    13861 tgacaacagc gtacccgttg actgtttgtc aaaaaatgaa accggcattc ccatcatgtg
    13921 accaaacagg cggcgacgca tggtcattac cacctttcct gatacccagg agatacagta
    13981 gctggagaca tagctggtga taccacgtaa aatcatcagc ccgatcacca ccagcggcat
    14041 ccacaccagc acggagcgat ctgttttacc aaagccatca tcaagaagtg gcttaaggag
    14101 cgataacatg aaggtatcgc tggctgcgtt gaggattaac gctacgcccg ccacgatcag
    14161 acccgcttta aaaggcgcaa tggttggcca cagtcggcgg aacgtctgcc acgtagagag
    14221 atctttgtcg ttatgcattc aaaaaaccag catttgttga aatagccgca tattctaccc
    14281 gttatccact ggcacgccaa accactgatg ataccaacga ggtaaaattt gctccctgag
    14341 gctgctaatc cgccagcctt gcgctgaaaa attgaccgct atttgcccct gatgaggagt
    14401 atcaagccac ttataaccct gttgttgata gcgatgttta actttgcttg agggcaatcg
    14461 ccatgcgtta tagcgcgatg ccgatgcgag tgccactttt ccattcactc gctgaattaa
    14521 tggcaacgat gatgaggtat tactgccatg gtgaggtacc tgaagcaatg ttgtctgcac
    14581 ttgctgccag taacggctta gcatcttttg ttcagcgggg acttcaatat caccggttag
    14641 aagaatgcta ttcgtcccgt catcaacctt aaccacacag gaatggttat ttcctttatc
    14701 gttgctagct tgtaaaggcc agtgcacgct gaaacgcaat ccttgccatt gccacgcttc
    14761 gccacgcaca cagggctgat gatgttccca gtttaacgga cttctgatcc ataacatcgg
    14821 ccatgtgtgc aatattgagt ccagccctcc ccggtgatcc agatgttcat gactcagaat
    14881 aacgccttcc ggttcaagat tatgccagtg gagccagggg ataatcagtt gttgcccact
    14941 atccccttcg ggccaggcca gtcctgtgtc atagagaatc gctttgccgt ttctggctat
    15001 caccattgcc agcccttgcc cgacatcaag catgtacacc tgccactcgt caggtcgagg
    15061 tttttgccac agcggccagc acatcagcaa gcctacagcc acacacattg ctggcaacgt
    15121 tcgccaggcg tttaatcgcc ataccaccag taagaaccat ggggaaaatg atagccattg
    15181 ccaacgttca gcaatgttga tccacccttc tggcaaactc tttaaccccc agaaaagtaa
    15241 agccaaagac cggtcggcaa gaaaccataa cccttgctcc aggattaacg gcccgcttaa
    15301 atgcacaacc atcgcggcga ggatcaacgg aaccgtgata aatgtcacca agggaattgc
    15361 taatagattt gcaataaacg aggtcagact aatgccatga aatatgacga tttgcacggg
    15421 cataagcaga agtgtgatac ccagttgcag atggatgagg gaaacaactg cacgcaatac
    15481 cggcggcagt tgccactcag gacagggaaa ccactgatac caaaaaatca gtgccgcgac
    15541 cgcagcggca gagagccata aactttgcga gagaatggca acaggatcca tcagcaaaat
    15601 tgccgccaga caacatatcc atacatccca gccactccac tgtcgcccac ttaacttaag
    15661 cattccccac gtagcaagcg ccaccacggt acgcaatgca ggaggttgca ttcccgtcaa
    15721 ccaggcataa aaagcagcac agcagatccc accaattaat ggtatttgcc agtggatcca
    15781 gcgcccaggc agaaaaattt gtccactgcg aatgagtccg gcagccagca acaccgcaaa
    15841 agcgatgtgc aatcccgata tcgccattaa atgcgccgtt ccagtatcgc gcatgatatt
    15901 tttgatttct ttggggacgg ataaccgttc ccccatacct aaaccaagaa taaccgcatt
    15961 ccacggatag ggttgcagcg ttgtttgtag tgacgccaga tactgtgcac gcaggctaca
    16021 attcggttca ataagacttg cctgcagaaa gcggccggtg agcggctgat gctgggcaat
    16081 ggcataacgc tggctatcaa agccgccatc attaagttga ccatgaacgg cacgaacttt
    16141 gagtttcatt gaccacagtt gtccggcaca aaccgcttgc ggaagatatt cgccatacag
    16201 cacaaggcct ggcgcaggga atatacgtcg accttgtaga taagtaattt gaccgtaatg
    16261 agtggtcatg ccgtcagtag cagtgatctc aacaattgca tcctgcgtcg cgccagttaa
    16321 ggtttctcct gcccacaaaa tttgctttgc tgataatatg ccccaaacga aaaacagcaa
    16381 cgtcagcgcg atataacgga cggttttaac aggtataaat gccagtacgc aggcgaagag
    16441 agtcagaaac gcaagggcta atgtcccagg caattggggc aaaatcagca acggaaaaat
    16501 tccgcaaatt atgcatacgt cgactgtcgt tattttcatg cactcctccc tgtttatgga
    16561 aagtgtgcaa ctttgttgag cggttgtctg gtgggaattt tgcgttcccc gaggcacatt
    16621 cagaagaatt taacgagagg gtgaaaactg ctcaaaactc tgcgaggcag cttccagttc
    16681 ctgattagag aaatgaaaaa agcacccgac aggtgctttt ctcgcgttca agtttgagta
    16741 aaaacttaac cgtaaatatt ggcgcgatcg cgcagttctt taccaggttt aaagtgagga
    16801 acgtattttc cttccagttc tactttatcg ccagtcttcg gattacgtcc ggtacgtggt
    16861 gcgcggtagt gcaaagagaa actgccgaaa ccgcggattt caatacgctc gccctgcgca
    16921 agagtcgagg ccatatgctc cagcatctct tttactgcat cttcaaccgt cttggcggga
    16981 atgtgcgatt gctgggtggc aagtctttct atcaattctg acttggtcat gattcctccg
    17041 gttccttaaa ggcaaattaa gcggctgctg ttgctgcatt gattaagggc ggccgtagcc
    17101 gcccttagtg cttgattaca ggacgaaacc tgcaatctgt caagtaaact caacaaactt
    17161 cggaataaaa atcccgaaga gtcagagaat tactcgcctt tagctgcttt gaaagcttca
    17221 gccattgcgt tgttggagaa gtttgcatct tcctgtttgt taacagttgc gattgcatct
    17281 ttctcgtcag cttcgtcttt cgcacgaaca gacaggctga ttgcgcggtt tttacgatca
    17341 acgccggtga atttagcttc aacttcgtcg ccaacgctca gaaccaaggt agcgtcttca
    17401 acgcggtcac gagatgcttc agaagcacgc aggtaacctt caacgccgtc agccagttct
    17461 acggttgcgc ctttagcgtc aactgcagtt actttaccgg ttacgatagc gcctttcttg
    17521 ttcagagcaa cccagttgtt gaacggatct tctgcgagct gtttaacgcc cagggagata
    17581 cgttcacgtt ctgcgtcaac ctgcagaaca actgcagcga tttcgtcgcc ttttttgtat
    17641 tcacgaactg cttcttcgcc tgcaacgttc caggagatgt cagacaggtg aaccaggccg
    17701 tcgatgccgc cgtccaggcc gatgaagata ccgaagtcag tgatagactt gattttacct
    17761 tcaacacggt cgcccttgtt gtgggtttcc gcgaactgct gccacgggtt agctttgcac
    17821 tgtttcagac ccagggagat acgacgacgt tcttcgtcga tatccagaac cataacttcc
    17881 actacatcgc caacgttaac aactttggac gggtggatgt ttttgttggt ccaatccatt
    17941 tcggaaacgt gtaccagacc ttcaacgcct tcttcgattt caacgaagca gccgtagtca
    18001 gtcaggttgg tcacgcgacc agtcagtttg gtaccttccg gataacgttt agcgatagct
    18061 acccacggat cttcgcccag ctgtttcagg cccagggata cacgggtacg ttcgcggtcg
    18121 aacttcagca ctttaacagt gatttcgtcg cccacgttga cgatttcgct cggatgctta
    18181 acgcgtttcc aggccatgtc agtgatgtgc agcaggccgt caacgccgcc cagatcaacg
    18241 aatgcaccgt agtcagtgag gttcttaacg atacctttaa cttccatgcc ttcctgcagg
    18301 ttttccagca gctgatcgcg ctctgcgctg ttttcggatt cgataacggc acgacgagaa
    18361 acaacaacgt tgttgcgctt ctgatccagc ttgattactt taaattcaag ctctttgcct
    18421 tccaggtgca gagtgtcacg caccggacga acgtctacca gagaacctgg caggaacgca
    18481 cgaataccgt tcagctcaac agtgaagccg cccttaactt tgccgttgat aacaccggta
    18541 acagtttcag catcttcgta agctttttcc agcgtgatcc aggcttcgtg acgtttagct
    18601 ttctcacggg acagcagagt ttcaccgaag ccgtcttcta ctgcgtccag agcaacgtca
    18661 acttcgtcac ctacctggat ttccagctcg ccctgggcgt ttttgaactg ctcagccggg
    18721 atggcggact cagatttcag accagcgtca accagtacta cgtctttgtc gatagcaaca
    18781 acaacgccac gaacgataga acccgggcgg gtttcgattt cttttaagga ctcttcaaag
    18841 agttgagcaa aagattcagt catgtttaat cttcaggttt atatttaacg tccacctggc
    18901 tccatgccgg atggggttgt ttaacatacc cgctgtcatt ccattgcaac gggggtactg
    18961 caaattcggt cgcttatgcg agagccaatt tctggcgcgc gtattgtagc gctttttcaa
    19021 tcacttgctc aatgcttaag gtggtggaat ccaacactaa agcatcggat gccggaacca
    19081 gtggcgctac cgctcggtta cgatcgcggt cgtcgcgttc tttgatctcg gccaaaaggc
    19141 gctcaaagtt aacactaaag cccttctcct gcaactgtag catgcggcga tgcgcacgtt
    19201 cttccgagga ggcgtcaagg aaaattttca ctggcgcatc agggaatacc accgttccca
    19261 tgtcgcggcc atcggcaatc aggcctggta attcgcgaaa cgcgcgttgg cgacgcaata
    19321 atgcttcacg aacgcgtggg aatgccgcga cttgtgaagc tgcattcgcc acttcctgag
    19381 tacgaatttc gccgctgaca tcttcccctt cgaggatcac ttccagattg ccattggttg
    19441 acacaaaacg tacatccaga tgggatgcca gcggtaccag cgcatcttct gacgcaacat
    19501 caacatggtg atgtaatgcc gccaacgcca gtacgcgata aattgcaccc gaatccagca
    19561 gatgccattg caatgcttcc gccatagcct tacacaaggt gcctttccct gcaccgcttg
    19621 ggccatcaat ggtaataacc ggggcaattg ccgtcatctt tatctcctta aaacaggcgt
    19681 accgttaaca taaccgcgcg cattatacgc gccgacgcca caaatcgtta acgttgtgtg
    19741 caaaaaccag ttttgtctgt attcagatgg ggatttattg cgcaattata gcgggctggc
    19801 agaagaccag cccgaaagat gacaatgatt tacttaccag aggcgatacg atcacggatg
    19861 tgttgcgcac gctctgttga tggtgggtga gagtcaaaca tggactgggt gcgaccgcca
    19921 tccagactag ccagtttttc aaagctgcca accagcccct gggtgctgat gccacgtttc
    19981 ttcaacagat caaaggagaa atcatctgct tcggactctt tatcacggga gtatttagca
    20041 ttaatagcgc cttctgcgat atcacccaat tgtgagcggg aaagctggga agccacaccg
    20101 ctggtagctg aaatggcatc gcgtgcggca acgatcgcat aagaagcttt catttcagcc
    20161 agcgagtgac ccaacgcgac gtggcccagt tcatggccca gaacaccttc aatttcgttg
    20221 tcattcatca tgtccatcag gccactgtag acacggacac agccgttcgc catcgcccat
    20281 gcgttgacgt cgctggtcat ataaacctta tagttgaccg gcgtgccatt aatattgtta
    20341 cccaatgctt tggcgatttt tgccagacgt ttaccgtact tgctggattt acttgcgact
    20401 tgattgccgc tgtccatttc ggcacagccc tgattggcaa tcgcttttgc atcggcatcg
    20461 cttaatgttg ctgctttata ggcatttaaa ccggagctga tagccatatt ggtatcaata
    20521 ccgtgggtat tttgacaccc tgtcagtaat gctgcagagg tcgcaatcgc cagcagtaat
    20581 ttagtattct tcatgtgaat catccgttga ttaatcatat ataaatatta tgtatcaaca
    20641 acttacattg acaagcgacg ctaccattaa tcaaaatgac tcacaaggtc cgaaaaaaaa
    20701 cgccgacaat g
//
LOCUS       DAEEKQ010000064        20578 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.064, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000064 DAEEKQ010000000
VERSION     DAEEKQ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.064"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="KLK57_002220"
     CDS             complement(<1..986)
                     /gene="cmtA"
                     /locus_tag="KLK57_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HBE0892583.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     LVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWVFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVATLVSFA"
     gene            complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="KLK57_002221"
     CDS             complement(1014..1457)
                     /gene="cmtB"
                     /locus_tag="KLK57_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HBE0892584.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTAASEKQLADIISRG"
     gene            complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="KLK57_002222"
     CDS             complement(1771..3762)
                     /gene="tkt"
                     /locus_tag="KLK57_002222"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HBE0892585.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIIGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            4040..4798
                     /gene="loiP"
                     /locus_tag="KLK57_002223"
                     /gene_synonym="yggG"
     CDS             4040..4798
                     /gene="loiP"
                     /locus_tag="KLK57_002223"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HBE0892586.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGIK"
     gene            complement(4941..5861)
                     /gene="speB"
                     /locus_tag="KLK57_002224"
     CDS             complement(4941..5861)
                     /gene="speB"
                     /locus_tag="KLK57_002224"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HBE0892587.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLTFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5999..7975)
                     /gene="speA"
                     /locus_tag="KLK57_002225"
     CDS             complement(5999..7975)
                     /gene="speA"
                     /locus_tag="KLK57_002225"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HBE0892588.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="KLK57_002226"
     CDS             complement(7984..8115)
                     /gene="yqgB"
                     /locus_tag="KLK57_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HBE0892589.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            8251..8466
                     /locus_tag="KLK57_002227"
     CDS             8251..8466
                     /locus_tag="KLK57_002227"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892590.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            8770..9924
                     /gene="metK"
                     /locus_tag="KLK57_002228"
     CDS             8770..9924
                     /gene="metK"
                     /locus_tag="KLK57_002228"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HBE0892591.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            10360..11754
                     /gene="galP"
                     /locus_tag="KLK57_002229"
     CDS             10360..11754
                     /gene="galP"
                     /locus_tag="KLK57_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HBE0892592.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            11831..12328
                     /locus_tag="KLK57_002230"
     CDS             11831..12328
                     /locus_tag="KLK57_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HBE0892593.1"
                     /translation="MKTSRLPIAIQQAVMRGLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            12423..13130
                     /gene="endA"
                     /locus_tag="KLK57_002231"
     CDS             12423..13130
                     /gene="endA"
                     /locus_tag="KLK57_002231"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HBE0892594.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMYNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            13210..13941
                     /gene="rsmE"
                     /locus_tag="KLK57_002232"
     CDS             13210..13941
                     /gene="rsmE"
                     /locus_tag="KLK57_002232"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HBE0892595.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            13954..14904
                     /gene="gshB"
                     /locus_tag="KLK57_002233"
     CDS             13954..14904
                     /gene="gshB"
                     /locus_tag="KLK57_002233"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HBE0892596.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTENGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            15013..15576
                     /locus_tag="KLK57_002234"
     CDS             15013..15576
                     /locus_tag="KLK57_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HBE0892597.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            15576..15992
                     /gene="ruvX"
                     /locus_tag="KLK57_002235"
     CDS             15576..15992
                     /gene="ruvX"
                     /locus_tag="KLK57_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HBE0892598.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(16167..17147)
                     /locus_tag="KLK57_002236"
     CDS             complement(16167..17147)
                     /locus_tag="KLK57_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HBE0892599.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFEAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRHGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSSKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQKREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMITFQQSFQQRVGEGRL"
     gene            17165..17869
                     /gene="yggS"
                     /locus_tag="KLK57_002237"
     CDS             17165..17869
                     /gene="yggS"
                     /locus_tag="KLK57_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HBE0892600.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            17887..18453
                     /gene="yggT"
                     /locus_tag="KLK57_002238"
     CDS             17887..18453
                     /gene="yggT"
                     /locus_tag="KLK57_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HBE0892601.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            18450..18740
                     /locus_tag="KLK57_002239"
     CDS             18450..18740
                     /locus_tag="KLK57_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HBE0892602.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQGNSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            18748..19341
                     /locus_tag="KLK57_002240"
     CDS             18748..19341
                     /locus_tag="KLK57_002240"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HBE0892603.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARYAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            19334..20470
                     /gene="hemW"
                     /locus_tag="KLK57_002241"
     CDS             19334..20470
                     /gene="hemW"
                     /locus_tag="KLK57_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HBE0892604.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
BASE COUNT         5011 a         5431 c         5292 g         4844 t
ORIGIN      
        1 gcaaaggaca ccagggtagc taccgtaaca ccggcaattg tcgccaggaa cgagcctttc
       61 ggcgtcagtg ccaggtaagc aaagatagac cccggacttg ggccagccac cagaccaccg
      121 tccagtaagt taaacaccca ggtgccagac ataccgcccg caatcatggc aataatggtc
      181 agcggcttca tcagcacata cgggaaatac agttcgtgga tcccaccgag gaagtgaata
      241 atcatcgccc cgggcgcaga acgtttactc atccctttac caaacaaggt aaacgccagc
      301 agcagcccta agccaggccc aggattggac gccaccataa agaagatgga tttaccgtta
      361 accgaagcct gttgcattcc cagcggataa tagacgccct gatcgatcgc attattgagg
      421 aaaagcactt tcgccggttc attgatgact gacagcagag gcagataacc cgcatgtacc
      481 agcgcctcaa tacactcttt gacgagagta ttggcaatta acaccgccgg gccgataact
      541 tcaaaaccca gcagacaaag gagcatcccc gcgataccta atgagaagtt attgataacc
      601 atctcaaaac cggcaggtat gcgtttttcc agtgccttat caacatattt tatgaccagc
      661 ccaccgagcg gccccataat cattgagcca aggaacatcg ggatctctgc gccgacgatc
      721 acaccaatag tacctattcc gcccatgacc gcgccgcgtt taccgccgac cagatgacca
      781 ccggtagaac caatcatcac gggcaataaa taggtaatca tcgggccgac aattttggcg
      841 aaatgttcgt taggcagcca accggtggga ataaataacg cagtaataaa accccaggca
      901 ataaaagcac caatattggg gatgaccatt gccgtcaaaa agcccccaaa agcctggacc
      961 tttgcacgag cagacttgtt ttccataata ttatcctgta gaggagagag taattatccg
     1021 cggctgatta tgtccgctaa ttgtttttct gatgctgctg ttaagagttg ttcgagtatt
     1081 tcttcttcgc acagtaattc acttaacgcc tgaatagcgc caatgtgcga atcggcatcg
     1141 gcagcagaaa ggccgatgag taattttatt ggttcgtcat tccccggaaa ataaacacct
     1201 tgttcaagta atgtcaaaga catcccggtt ttaagcgccc cacattccgg tcgcgcatga
     1261 ggcattgcca cgcctggtgc gagaatataa taagggccat tgttaatggt ggaatcttta
     1321 attgcctgaa tgtaattctc gctgatatag tttttatcca gcaatgatac catcgagaaa
     1381 tcgatagctt cctgccaatc ttttgctgaa tgtataactg agattgatga ttctggaaaa
     1441 tagtcactaa gccgcataaa ttatccttat tttattttta gggtaccgtc cggcggtaaa
     1501 aataaaatac tcgtgcggtt taatgaaagc aattgagcag ggaaaccggt gttttgaaaa
     1561 cagtgaatta ttaatttctt atataacatt gagttataga tataacaaca aatgtcaata
     1621 cgcatatcgt gatgcatatt acataattga tatttattgc atataaatat ttgtgatcta
     1681 caacacgctt tatctattgc ttgtccgcaa acggacatat caaggtaata aaaaaggtcg
     1741 ccgaagcgac ctttttaccc gaaatgctaa ttacagcagt tcttttgctt tcgcaacgac
     1801 gttgtcgacg gtgaagccga actcttcgaa cagcaactct gccggagctg actcaccgaa
     1861 ggtggtcata ccgatgatag cgccattcag gccaacatac ttgtaccagt agtcagcaat
     1921 acccgcttct accgcaacgc gtgcagtaac cgctttcggc agtacggatt cacggtaagc
     1981 cgcatcctgc ttgtcgaatg catcggtaga cggcatggaa accacgcgcg ccttcacgcc
     2041 ttcggcagtc agtttttcgt aggcggcaac agccagttca acttccgaac cggtcgcgat
     2101 gaagatcagt tccggctgac cagcacagtc tttcagcaca taaccaccgc gcgcgatgtt
     2161 tgccagttgc tcttcagtac gttcctgctg cgccagattc tgacgggaga ggataagcgc
     2221 ggtcggaccg tcctgacgct caacaccgta tttccacgcg accgcggatt caacctggtc
     2281 acacggacgc catgtagaca tgttcggggt cacgcgcaga gaagcgacct gctcaaccgg
     2341 ctggtgagtc gggccgtctt cgcccagacc gatggagtcg tgggtgtaaa ccatcacctg
     2401 acgctgtttc atcagcgcag ccatacgtac agcgttacgt gcgtattcga caaacatcag
     2461 gaaggtggag gtgtacggca ggaagccacc gtgcagggag ataccgttag caatcgcggt
     2521 cataccgaac tcgcgaacac cgtagtggat gtagttaccc gcagcatctt cgttgattgc
     2581 tttagaacca gaccacaggg tcaggttaga cggtgccagg tcagcggagc cgccgaggaa
     2641 ttccggcaac agcgggccga acgcttcaat agcattctga gacgctttac ggctggcgat
     2701 tttcgccgga ttagcctgca gtttagcgat aaactctttc gctttggcgt cgaagtcaga
     2761 cggcatttcg cctttcatac ggcgggtaaa ttcagccgct tcctgcggat aagctttcgc
     2821 gtaagccgca aacttctcat tccatgcaga ttctttcgcc tggcctgctt ctttcgcatc
     2881 ccactgcgca tagatttcag acgggatttc gaacggtgcg tatttccagc ccagctgttc
     2941 gcgggtcaga gcgatttctg catcacccag cggcgcaccg tgggagtcgt gagtaccggc
     3001 tttgttcggg gaaccgaaac cgatgatggt tttgcacatc agcagggacg gtttgtcagt
     3061 cactgcgcgc gcttcttcta ctgcgcgttt gatggatgct gcgtcatgac cgtcgatgtc
     3121 gcgaataacg tgccagccgt aagcttcgaa acgcattgcg gtgtcgtcgg tgaaccagcc
     3181 ttcaacgtga ccatcaatag agataccgtt gtcatcgtag aacgcaatca gtttacccag
     3241 cttcagcgta cccgccagag agcaaacttc gtgggagatg ccttccatca tgcagccgtc
     3301 gcccatgaag gcgtaggtgt agtggtcgac aatgtcgtgg cccggacggt taaactgcgc
     3361 cgccagcgtt ttttctgcaa tcgccatacc gactgcgttg gcaataccct gacccagcgg
     3421 accggtggtg gtttccacac cagcggtata acctacttcc gggtggcccg gagttttaga
     3481 gtgcagctga cggaagtttt tcagttcttc catcggcaga tcgtaaccgg tgaggtgcag
     3541 caggctgtag atcagcatgg agccgtggcc gttggacagc acgaagcggt cacggtcagc
     3601 ccaggacgga ttctgcgggt tgtgtttcag gaaatcacgc cacaggactt cggcaatgtc
     3661 agccataccc ataggggcac ccgggtgacc ggatttggct ttctgtactg cgtccatgct
     3721 cagcgcacga atagcattgg caagctcttt acgtgaggac attttgactc cagatcggat
     3781 gatgaagggc acgcccttaa cgacttgacg acagcgcgtt ttgggctacg ccggaaaatt
     3841 tgccaacaat ttaccgcaag ccgcgcgtca tgtacatgga acatcctttt gccgcttcag
     3901 aaatctctgg atcatgctcg catgttgcgc aatctactcg cccgtccgct gcgcttttcc
     3961 ttatactgag actgagcgtc gattcacctg caaacggcgc atttttagaa taatcctgac
     4021 cttgtgcgga agagaaaaca tgaaaattcg cgccttattg gtagcaatga gcgtggcaac
     4081 ggtactgact ggttgccaga atatggactc caacggactg ctctcatcag gagcggaagc
     4141 ttttcaggct tacagtttga gtgatgcgca ggtgaaaacc ctgagcgatc aggcatgtca
     4201 ggagatggac agcaaggcga cgattgcgcc agccaatagc gaatacgcta aacgtctgac
     4261 aactattgcc aatgcgctag gcaacaatat caacggtcag ccggtgaatt acaaagtgta
     4321 tatggcgaag gatgtgaacg cctttgcaat ggctaacggc tgtatccgcg tctatagcgg
     4381 gctgatggat atgatgacag ataacgaagt cgaagcggtg atcggtcacg agatggggca
     4441 cgtggcgtta ggccatgtga agaaagggat gcaggtggcg cttggtacaa atgccgtgcg
     4501 agtagctgcg gcctctgcgg gcgggattgt cggcagttta tctcaatcac aacttggtga
     4561 tctgggcgag aaattagtca attcgcaatt ctcccagcgc caggaagcag aagccgatga
     4621 ttattcttac gatcttctgc gccaacgcgg catcagcccg gcaggtcttg ccaccagctt
     4681 tgaaaaactg gcgaaactgg aagaaggccg ccaaagctca atgtttgacg accatccggc
     4741 atccgccgaa cgcgcccagc atattcgcga tcgcatgagc gcggatggga ttaagtaaag
     4801 cctggtggtg tcgaacgtag gtcagataag gcgttcacgc cgcatctgac atcaatggca
     4861 catttgttac cctgtgcgca ttgccggatg cgatgctgac tgcatcttat ccggcctacg
     4921 ggtggcatcg catctggtgc ttactcgccc tttttcgccg cttggatata cagcatctcc
     4981 agcgccagcg tcgccgcagc cagagcggtg atttccgact gatcgtatgc cggagccact
     5041 tccactacgt ccatcccaac aatgttgaga tctttcaggc cgcgtaccag tttaatagcg
     5101 cgatcggagg tcaggccgcc aatcactggc gtaccggtgc ctggtgcaaa agcaggatcc
     5161 aggcagtcga tatcaaaagt caggtaaacc ggcatatcac ccacaatctg tttcacttgg
     5221 gcgataacgt catccacgct gcgatcgttc acctggcagg cgtccagcac ggtaaagccg
     5281 ttgtctttat caaactcggt acgaatacca atctgcacgg aatgattcgg gtcgatcaga
     5341 ccttctttcg gcgcggtata gaacatagtg ccgtggtcaa attcacaacc gttcgcatag
     5401 gtatcggtgt gggcgtcaaa gtgtaccagc gccattttgc cgaaatgctt cgcatgagca
     5461 cgcagcagcg gcagcgtaac aaagtggtca ccaccgaagg tgagcatacg cttaccggca
     5521 gccagcagct tctcggcgtg cgcctgcagc ttttcgctca tctcacgggc atcgccaaag
     5581 gcatatacca gatcgccgca gtccacgacg ttcagacgct cacgcatgtc gaaattccac
     5641 gggaagcggt tgtgttccca ggccagattc gtcgaaacct gacggatcgc tgccggaccg
     5701 tggcgaccac ccgcacgacc agaagtggcc atatcgaacg gcacgccagt aatcacccag
     5761 tctgcatcgc tgtcatacgg ctggaagttc atcggcaggc gtaaaaaacc aaaggcattg
     5821 gaaaccagtg agttatcgta ttgatgacct aaggtgctca tggatggact cctattacaa
     5881 agtcgatata aaaaaaccct tccgcgtcgt taggcccgac gaggaagggt tggatttgtc
     5941 acaataaatt gtggcggatt atcaccgcta aattaagcgg attcaagtaa cacaggactt
     6001 actcatcttc aagataagta taaccgtaca aacctgcctc gaactcttca aggaactgtt
     6061 gttgcagttc agcatcaaga tcggttttct tcacttgatc gcggaactgg gttaacagcg
     6121 ttttcggatc gagctgtaca tattgcagca tgtccgccac ggtatcgcct tcgtcagaca
     6181 gttctacttc tacgctaccg tcagggaaca cgaacacgtc aaccgcttcg gtatcaccga
     6241 acaggttgtg catattgcca aggatctcct gatatgcgcc gaccataaag aaaccgagca
     6301 tcggcggatt ctctggatcg tactccggca ttggcattgt cgtggcaata ccatcaccat
     6361 caatatagtg gtcgatagca ccgtcagagt cacaggtaat atccagcagc acagcgcgac
     6421 gttccggcac ttgatccagc ccttccagcg gcagaaccgg gaacaactgg tcgatccccc
     6481 atgcgtccgg cattgactgg aacagcgaga aattgacgta cattttgtcc gccatacgtt
     6541 cctgcagttc gtcgattatc ggacgatgcg cacggttttg cggatccagt tgcttctgca
     6601 cttcatggca catgctcaga tagagttgtt ctgcccatgc acgttcttgc aggctaaagg
     6661 tgccggaaga gtagccgata tgaatgtcgt gcagatccat ctggctgtcg tgtaaccatt
     6721 cacgcaaaga acggcgagtt cccggttcgt gcatttcctg ccaggtttcc cacatgcttt
     6781 gcagcgcgcg cggcgcatct tctgcgggcg cggtcggcac cgtatattcg ttacgttcca
     6841 cgccgatgat attcgacacc agcacggtat ggtgcgcagt caccgcacgg cccgattcgg
     6901 tgattaccgt cggatgcggc agaccatttt cttcacacgc atcgccaatc gcccagatga
     6961 tgttgttggc gtattcattg aggccatagt ttacggagca gtcagactgc gaacgggtgc
     7021 cttcatagtc cacgcccaga ccgccgccga cgtcgaagca ctgaatattg acgcccagct
     7081 tgtgcagttc cacatagaaa cgcgcggatt cacgaacgcc cgtggcgata tcgcgaatat
     7141 tcgccatctg cgaaccgagg tggaagtgca gtagttgcag gctgtcgaga cgcccggctt
     7201 cacgcagggt ttcaaccagt tgcagtacct gagtcgcagc caggccgaac ttcgattttt
     7261 ccccgccgga ggactgccat ttacccgagc cctgcgaagc cagacgtgca cgcacgccca
     7321 gacgaggaac gacattcaga cgttctgctt catccagcac aatggcgatt tctgacatct
     7381 tctcaatgac cagatagacc ttgtgcccca tcttctcgcc aattaatgcc aggcggatat
     7441 attcgcggtc tttataaccg ttgcagacga tgacgctacg ggtcatgcca gcatgtgcca
     7501 gtactgccat caactcggct ttggaaccgg cttccagacc cagcggttcg cccgaatgaa
     7561 tcagggactc aatcacgcgg cggtgctggt taactttgat cggataaaca aggaagtaat
     7621 cgccgttata gccgtaggat tccctcgcac gtttgaacgc ggcgttaatg gaacgcaaac
     7681 ggtgctgcag gatctgtggg aaacagaaca gtgcaggcag acgctgaccc tgtgcttcac
     7741 gagttttcac taactgcgcg agatcgacgc gagcttccgg gacgtccggg tccgggcaca
     7801 cgctgatgtg gcccagctcg ttaacgtcat agtagttatt gccccaccag gcaatattgt
     7861 aagtacgcag catcttgctg gcttcctggg agctcattgc aacctcctgc atggagcgta
     7921 gtacaccgtg ttcgcccgct gacgaaggca aacccataga catgtcgtca gacatagcga
     7981 acctcaaatt attttattaa gtgtaaaaca gttaacgact atcgcagcct ggaactgcga
     8041 taacaaccca taagcacatg gattttccag cagtgaatgc tgacgctcca actgcgcaac
     8101 cggtttcttt ttcataacat tattaagcac ataaccgaac gtaagtgtga cagttcggcg
     8161 aaaccacgag aaaactcttg tttttacaag agcgcccttg ttcagtcctc agtaactgta
     8221 accagctctt gaatcctgag aagcgccgag atgggtataa catcggcagg tatgcaaagc
     8281 agagatgcag agtgcgggga acgaatcttc accagaacgg tgagacaggt taagcagcag
     8341 acaacggttc attatttcgt atcacctcca cggccgcctg ttaagacgaa cccacaagcc
     8401 aaaactctga tttcaacccg gctggaagtg gcaacacgaa agaaacgtcg tgtgcttttt
     8461 atttaagccg cgcgccgcgt tttatacccc acaatggcag aaaattgcaa aagataaata
     8521 cgcagaatgc cggcattgtc aggaaaaatt tccagccacg tttttaacag aatgagacac
     8581 gattcaaaaa aaagtggaaa tcgggtgaag aattgaccta aaatagccat ccagatgtta
     8641 atccatccat accgattaac actcagactg ccagtgtttt taacctgcag agtcgtggta
     8701 ggatccgcta ccacagaaaa tccacacaac agtttgagct aaccaaattc tctttaggtg
     8761 atattaaata tggcaaaaca cctttttacg tccgagtccg tctctgaagg gcatcctgac
     8821 aaaattgctg accaaatctc tgatgccgtt ttagacgcga tcctcgaaca ggatccgaaa
     8881 gcacgcgttg cttgcgaaac ctacgtaaaa accggcatgg ttttagttgg cggcgaaatc
     8941 accaccagcg cctgggtaga catcgaagag atcacccgta acaccgttcg cgaaattggc
     9001 tatgtgcatt ccgacatggg ctttgacgct aactcctgtg cggttctgag cgctatcggc
     9061 aaacagtctc ctgacatcaa ccagggcgtt gaccgtgccg atccgctgga acagggcgcg
     9121 ggtgaccagg gtctgatgtt tggctacgca actaatgaaa ccgacgtgct gatgccagca
     9181 cctatcacct atgcacaccg tctggtacag cgtcaggctg aagtgcgtaa aaacggcact
     9241 ctgccgtggc tgcgcccgga cgcgaaaagc caggtgactt tccagtatga cgacggcaaa
     9301 atcgttggta tcgatgctgt cgtgctttcc actcagcact ctgaagagat cgaccagaaa
     9361 tcgctgcaag aagcggtaat ggaagagatc atcaagccga ttctgcccgc tgaatggctg
     9421 acttctgcca ccaaattctt catcaacccg accggtcgtt ttgttatcgg tggcccgatg
     9481 ggtgactgcg gtctgactgg tcgtaaaatt atcgttgata cctacggcgg catggcacgt
     9541 cacggtggcg gagcattctc tggtaaagat ccatcaaaag tggaccgttc cgcagcctac
     9601 gcagcacgtt atgtcgcgaa aaacatcgtt gctgctggcc tggccgatcg ttgtgaaatt
     9661 caggtttcct acgcaatcgg cgtggctgaa ccgacctcca ttatggtaga aactttcggt
     9721 actgagaaag tgccttctga acaactgact ctgctggtac gtgagttctt cgacctgcgc
     9781 ccatacggtc tgattcagat gctggatctg ctgcacccga tctacaaaga aaccgcagca
     9841 tacggtcact ttggtcgtga acatttcccg tgggaaaaaa ccgacaaagc gcagctgctg
     9901 cgcgatgctg ccggtctgaa gtaatctttc ttcacctgct ttcaaaggcc agcctcgcgc
     9961 tggccttttt cttttacctc accgcggtgg ataggcattc acgccgcatc cggcaaaaag
    10021 ctgcccgcac aataacatca ttcttcctca acacgtttta tcgcaaatta tcatcactac
    10081 tgaaaccgat tacaccaata acaccagcca aatatttgta atattttcat attattattc
    10141 agttttcaca gttgttacat ttcttttcag taaagtctta attgcagata acagcgttta
    10201 atctatgatg atataactca attattttca tgcacttaaa ccataactaa gataaatgtt
    10261 agtgtaagcg attacactga tgtgatttgc ttcacatctt tttacgtcgt actcacctat
    10321 cttaattcac aataaaaaat aaccatattg gagggcatca tgcctgacgc taaaaaacag
    10381 gggcggtcaa acaaggcaat gacgtttttc gtctgcttcc ttgccgctct ggcgggatta
    10441 ctctttggcc tggatatcgg tgtaattgct ggcgcactgc cgtttattgc agatgaattc
    10501 cagattactt cgcacacgca agaatgggtc gtaagctcca tgatgttcgg tgcggcagtc
    10561 ggtgcggtgg gcagcggctg gctctccttt aaactcgggc gcaaaaagag cctgatgatc
    10621 ggcgcaattt tgtttgttgc cggttcgctg ttctctgcgg ctgcgccaaa cgttgaagta
    10681 ctgattcttt cccgcgttct actggggctg gcggtgggtg tggcctctta taccgcaccg
    10741 ctgtacctct ctgaaattgc gccggaaaaa attcgtggca gtatgatctc gatgtatcag
    10801 ttgatgatca ctatcgggat cctcggtgct tatctttctg ataccgcctt cagctacacc
    10861 ggtgcatggc gctggatgct gggtgtgatt atcatcccgg caattttgct gctgattggt
    10921 gtcttcttcc tgccagacag cccacgttgg tttgccgcca aacgccgttt tgttgatgcc
    10981 gaacgcgtgc tgctacgcct gcgtgacacc agcgcggaag cgaaacgcga actggatgaa
    11041 atccgtgaaa gtttgcaggt taaacagagt ggctgggcgc tgtttaaaga gaacagcaac
    11101 ttccgccgcg cggtgttcct tggcgtactg ttgcaggtaa tgcagcaatt caccgggatg
    11161 aacgtcatca tgtattacgc gccgaaaatc ttcgaactgg cgggttatac caacaccaca
    11221 gagcaaatgt gggggacagt gattgtcggc ctgaccaacg tacttgccac ctttatcgca
    11281 atcggccttg ttgaccgctg gggacgaaaa ccaacgctaa cgctgggctt cctggtgatg
    11341 gctgctggca tgggcgtact cggtacaatg atgcatatcg gtatccactc tccgtcggcg
    11401 cagtatttcg ccatcgccat gctgctgatg tttattgtcg gttttgccat gagtgccggt
    11461 ccgctgattt gggtactgtg ctccgaaatt cagccgctga aaggccgcga ttttggcatc
    11521 acctgctcca ctgccaccaa ctggattgcc aacatgatcg ttggcgcaac gttcctgacc
    11581 atgctcaaca cgctgggtaa cgccaacacc ttctgggtgt atgcggctct gaacgtactg
    11641 tttatcctgc tgacattgtg gctggtaccg gaaaccaaac acgtttcgct ggaacatatt
    11701 gaacgtaacc tgatgaaagg tcgtaaactg cgcgaaatcg gcgctcacga ttaatctcct
    11761 caggcttcct cccgtcgcgg aggaagccgc ctcttgcagt catcttttct tcgctctatc
    11821 ctctgccgct atgaaaacat cccgtctccc tatcgccatc caacaggccg ttatgcgcgg
    11881 cctgcgggaa aaactcgccc aggccaacct gaagctaggg cgtaactacc cggagccaaa
    11941 actctcttac acccagcgcg gaacctccgc cggaacagcc tggctggaaa gctatgaaat
    12001 ccgcctcaat cccgttttgc tgttggaaaa cagtgaagct tttattgaag aagtggtacc
    12061 gcacgaactg gcacatttgc tggtatggaa acatttcggc cgcgtagcgc cacatggcaa
    12121 agagtggaag tggatgatgg aaagcgtgct gggtgttccc gcccgtcgta cgcatcagtt
    12181 cgaactgcaa tccgtgcgtc gcaacacctt cccctaccgc tgcaagtgcc aggagcatca
    12241 gcttaccgta cgccgccata atcgcgtagt tcgtggcgag gccgtctatc gctgtgttca
    12301 ctgcggtgaa cagctggttg cgaaataacc atctgaacta tcaggaactt tcctgatctg
    12361 gctgattgca taccaaaaca actttcgcta cgttgctggc tcgttttaac acggagtaag
    12421 tgatgtaccg ttatttgtct attgctgcgg tggtactgag cgcagcattt tccggcccgg
    12481 cgttggccga aggtatcaat agtttttctc aggcgaaagc cgcggcggta aaagtccacg
    12541 ctgacgcgcc cggtacgttt tattgcggat gtaaaattaa ctggcagggc aaaaaaggcg
    12601 ttgttgatct gcaatcgtgc ggctatcagg tgcgcaaaaa tgaaaaccga gccagccgcg
    12661 tagagtggga acacgtcgtt cccgcctggc agttcggtca ccagcgccag tgctggcagg
    12721 acggtggacg taaaaactgc gctaaagatc cggtctatcg caagatggaa agcgatatgt
    12781 ataacctgca gccgtcagtc ggtgaggtga atggcgatcg cggcaacttt atgtacagcc
    12841 agtggaatgg cggcgaaggc cagtacggtc aatgcgccat gaaggtcgat ttcaaagaaa
    12901 aagctgccga accaccagcg cgtgcacgcg gtgccattgc gcgcacctac ttctatatgc
    12961 gcgaccaata caacctgaca ctctctcgcc agcaaacgca gctgttcaac gcatggaaca
    13021 agatgtatcc ggttaccgac tgggagtgcg agcgcgatga acgcatcgcg aaggtgcaag
    13081 gcaatcataa cccgtatgtg caacgcgctt gccaggcgcg aaagagctaa cctacactag
    13141 cgggattctt tttgttaacc cctaccccac gcgtacaacc gcgtggggag acgacgcgga
    13201 tttttaacta tgcgtatccc ccgcatttat catcctgaac cactgaccag ccattctcac
    13261 atcgcgcttt gcgaagatgc cgccaaccat atcgggcgcg tactgcgcat ggggccgggg
    13321 caggcgttgc aattgtttga cggtagcaac caggtctttg acgccgaaat taccagcgcc
    13381 agcaaaaaaa gcgtggaagt gaaggtgctg gaaggcaaga tcgacgatcg cgaatctccg
    13441 ctgcatattc acctcggtca ggtgatgtcg cgtggtgaaa aaatggaatt tacgatccag
    13501 aaatcgatcg aactcggtgt aagcctcatt acgccacttt tttctgagcg ctgcggcgtt
    13561 aaactggata gtgaacgtct gaacaagaag ctccagcagt ggcagaagat tgcaattgct
    13621 gcctgtgagc agtgtggtcg taaccgggtg ccggaaatcc gtccagcgat ggatctggaa
    13681 gcctggtgtg cagagcagga tgaaggactg aaactgaatc ttcacccgcg cgccagtaac
    13741 agcatcaata cgttgccgtt accggttgaa cgcgtccgcc tgctgattgg cccggaaggc
    13801 ggtttatcgg cagatgaaat tgccatgact gcccgctatc aatttactga tatcctgttg
    13861 ggacctcgcg ttttgcgtac agagacaact gcgctcaccg ccattaccgc gctacaagta
    13921 cgatttggcg atttgggcta acggagaaga ataatgatca agctcggcat cgtgatggac
    13981 cccatcgcaa acatcaacat caagaaagat tccagtttcg ctatgttgct ggaagcacag
    14041 cgtcgtggtt acgaacttca ctatatggag atgggcgatc tgtatctgat caatggcgaa
    14101 gcccgcgccc atacccgcac gctgaacgtg aagcagaact acgaagagtg gttttcgttc
    14161 gtcggtgaac aggatctgcc gctggccgat ctcgatgtga tcctgatgcg taaagacccg
    14221 ccgtttgata ccgagtttat ctacgcgacc tatattctgg aacgtgccga agagaaaggg
    14281 acgctgatcg ttaacaagcc gcagagcctg cgcgactgta acgagaaact gtttaccgcc
    14341 tggttctctg acttaacgcc agaaacgctg gttacgcgca ataaagcgca gctaaaagcg
    14401 ttctgggaga aacacagcga catcattctt aagccgctgg acggtatggg cggcgcgtcg
    14461 attttccgcg tgaaagaagg cgatccaaac ctcggcgtga ttgccgaaac cctgactgag
    14521 aatggcactc gctactgcat ggcacaaaat tacctgccag ccattaaaga tggcgacaaa
    14581 cgcgtgctgg tggtggatgg cgagccggta ccgtactgcc tggcgcgtat tccgcagggg
    14641 ggcgaaaccc gtggcaatct ggctgccggt ggtcgcggtg aacctcgtcc gctgacggaa
    14701 agtgactgga aaatcgcccg tcagatcggg ccgacgctga aagaaaaagg gctgattttt
    14761 gttggtctgg atatcatcgg cgaccgtctg actgaaatta acgtcaccag cccaacctgt
    14821 attcgtgaga ttgaagcaga gtttccggtg tcgatcaccg gaatgttaat ggatgccatc
    14881 gaagcacgtt tacagcagca gtaacccacc ttagcgagaa ggatctcgtt gagactctga
    14941 gtgacagcgc ccttctttcc acgcatactg ggcgctgttg cttttttgaa ccaggaaaca
    15001 gaacctctga caatgaattt acagcatcac tttcttattg ccatgcctgc tctccaggat
    15061 ccgattttcc gtcgttccgt ggtctacatt tgcgaacata ataccaatgg tgcaatgggg
    15121 atcatcgtca acaagccgct ggaaaatctc aaaattgaag ggattctgga aaagctgaag
    15181 atcacgccgg agccgcgtga tgaatcaatc cgtctggata aaccggttat gctcggcggt
    15241 ccgctggctg aagatcgcgg gtttattttg catactccgc cctccaattt tgcttccagc
    15301 attcgcattt cagacaacac agtgatgacc acttcccgcg atgtgctgga aacgctcggc
    15361 accgataaac aaccgtctga cgtattggtg gctctaggtt atgcctcctg ggaaaaaggt
    15421 caactggaac aagaaattct cgataacgcg tggctaacgg ccccggcgga tctgaatatt
    15481 ctgttcaaaa cgccgattgc cgaccgctgg cgcgaggcgg caaaactgat tggtgtggat
    15541 attctcacca tgcctggtgt ggcagggcac gcctgatgag tggaacctta ctcgccttcg
    15601 acttcggcac caaaagcatt ggcgtagcgg tcggccaacg cattaccggc actgctcgcc
    15661 ctttgcctgc aattaaagca caggacggta cgccggactg gaacctcatc gaacgtttac
    15721 tgaaagagtg gcagccggac gaaatcatcg tcggtttgcc gctgaatatg gacggcaccg
    15781 agcaaccatt gactgccaga gcgcgtaaat ttgccaaccg tattcatggt cgtttcggtg
    15841 ttgaagtaaa gctccatgac gaacgtctta gcaccgtgga agcccgttcc ggtctgtttg
    15901 aacagggcgg ctatcgggcg ctcaacaaag gcaaagtcga ctctgcctct gcggttatta
    15961 ttctcgaaag ctatttcgag cagggatatt aaggcgattt aaaagcctgg cggatagtcg
    16021 ttaccgccat cttgccggat gcggcgtgaa cgcctatccg gcctacatgt ggcagcgatt
    16081 gtaggtctga taagacgcgc aaacgtctca tcagacgttt attgcctttg taggcccgat
    16141 aagcttgcac atcgggcatg gcaacgtcac aaacgccctt cccccactcg ctgctgaaaa
    16201 ctctgctgaa acgttatcat ccccacctgc tgcccggttt gaataacatg cggtaactgg
    16261 tgggttttcc cttcgcgaat caaattcccc accgcgggag tgttaatcaa taattcaaac
    16321 agcgccacgc gtccttcctg tttatccact tccagctttt gtgacaacac ggcccgtaaa
    16381 ctacctgcca gttgattacg tacgggatct ttttcctgcg ccggaaatga atccaccagt
    16441 cgctcaactg cctgcgcggc accacgcgta tgtaatgttg ccagcaccaa atgcccggtt
    16501 tctgccgccg tcagtgccag acggattgtc tcgctgtcac gcagctctcc gagcaaaatc
    16561 acatcaggat cttcccgcaa tgcggcccgc aatcccgatg cgaacgtcat acagtgcaaa
    16621 ccaatttccc gcttctggat caaacatcgc tggctggcat agatatattc aacaggatct
    16681 tccagcgtca gaatatgcgc atcggcatgt tgattgagat agccaaccat cgccgccagc
    16741 gtggtagatt tgctgctccc cgtcgcccct gtcaccagaa tcaggccatt ctcactcttg
    16801 agtaattccg gcaataccgt tggtgcgcca agctgttcga gctgcgggca gtgcgaaggt
    16861 aacagccgta acgccagcga aatgccatgc cgttgcgcaa atgcgctgcc acgcaatcgc
    16921 tggttttccg ccagcgacac ggcaaaatcc agctggccat tttccagcaa tattgcccgc
    16981 tgatcgtcat ccagccactc ccgcagtagc tcttcgacgt ccggcgcctc aaacggcgca
    17041 gcttccatta gcccgcgaat gcgccatcgt gcgggccagg cgctgcacag gtgtagatcc
    17101 gagacgttat gctttacact aagggccaca atttcttcca tattcatact aagatcctcg
    17161 gaaaatgaac gatattgcgc ataacctggc acaggtccgg gacaaaatct cagcggctgc
    17221 aacgcgttgc ggccgttctc cagaagaaat tacgctgctt gcagtcagta aaacaaaacc
    17281 tgcgagcgcc atcgcagaag ccattgatgc cgggcagcgt caatttggtg aaaactacgt
    17341 tcaggaaggg gtagataaaa ttcgccactt tcaggaactg ggcgtaacag gattagaatg
    17401 gcattttatt ggcccgttgc agtctaataa aagccgcctg gtggcagagc atttcgactg
    17461 gtgtcatacc atcgaccgtt tgcgcattgc tacccgcctc aacgatcagc gcccggcaga
    17521 acttccccct cttaacgtac tgattcaaat taatattagt gatgaaaaca gtaagtccgg
    17581 gattcaactg gcagaactgg acgagctggc agcggcggtc gctgaactac cgcgtttacg
    17641 tctgcgcggg ctgatggcaa tccctgcgcc tgagtcagaa tatgtaaggc agtttgaagt
    17701 tgcacgccaa atggctgtag catttgccgg actgaaaacg cgctacccgc atatcgacac
    17761 gctctctctg ggaatgtcgg acgatatgga agccgccatt gcggcaggta gcacgatggt
    17821 tcgtatcggc actgcaattt ttggtgcgcg tgattactct aaaaaataag gaattaaagg
    17881 aacgccatga atacgttgac tttcctgctt tcaacggtca ttgagctgta taccatggtg
    17941 ctgttattac gcatctggat gcagtgggct cattgtgatt tttacaaccc cttctcacag
    18001 tttgtagtga aggtaacgca gccaattatc gggccactgc gccgcgttat tccggcaatg
    18061 ggccccattg acagcgcctc gctgctggtt gcctatattc tcagttttat caaagccatc
    18121 gtgctgttta aagtggtgac cttcctgcca atcatctgga ttgctggttt actgattctg
    18181 ctgaaaacca tcggcctgct gattttctgg gtcctgctgg tgatggcgat tatgagctgg
    18241 gtaagccagg gtcgtagtcc gattgaatac gtgctgattc agctggccga tccgctgctg
    18301 cgcccgattc gccgcctgct accggcaatg ggtgggattg atttctcgcc gatgatcctc
    18361 gttctgctgc tgtatgtcat caatatgggt gtcgcagaag tattacaggc aaccggaaac
    18421 atgctgctgc cggggctgtg gatggcgtta tgagtgccgt aacagttaat gatgacggtc
    18481 tggttttacg gctctatatt cagccgaaag ccagccgtga ttctattgtc ggtttacatg
    18541 gcgacgaagt taaagtcgcc attaccgcgc cgccggttga cggccagggc aacagtcatc
    18601 tggtgaagtt tctcggtaag caattccggg ttgccaaaag ccaggtggtg attgaaaaag
    18661 gcgaacttgg ccgccacaaa caaattaaaa tcattaatcc gcaacaaatc ccgccagaaa
    18721 tcgcggcgtt aattaattag gtatcctatg caaaaagttg tcctcgcaac cggcaatgcc
    18781 ggtaaagtgc gtgagctggc gtcgctgctt agcgacttcg gtcttgatat cgtggcccaa
    18841 acagacctcg gcgtagattc cgctgaagaa accggcctga cctttatcga aaacgcgatt
    18901 ctgaaagcgc gctatgcggc aaaagtgacc ggtttaccgg caattgccga cgactctggt
    18961 ctggcggtag atgcgctggg tggcgcgccg ggtatttact ccgcgcgtta ttccggtgaa
    19021 gacgcgaccg atcaaaagaa tctgcaaaaa ctgttggaaa cactgaaaga cgtaccggac
    19081 gaccaacgtc aggcgcgttt ccactgcgtg ctggtatatc tgcgtcacgc ggaagatccg
    19141 actccgctgg tatgccacgg cagctggccg ggcgtgatta ctcgcgaacc agcgggcact
    19201 ggtggctttg gttatgatcc aatcttcttc gtaccttccg aagggaaaac cgccgccgaa
    19261 ctgacccgcg aagaaaagag cgccatttcc caccgtggtc aggcgttgaa actgctgctg
    19321 gacgctttac gtaatggtta aattaccgcc gctgagtctc tacattcaca tcccgtggtg
    19381 cgtgcagaaa tgcccgtact gcgatttcaa ctctcacgcg ttgaaaggag aagtgccgca
    19441 cgacgattat gttcagcatc tgctttgcga tctggacaat gatgtggctt acgctcaggg
    19501 ccgtgaagta aagacaatct ttattggcgg tggtacgccg agcctgcttt ccggcccggc
    19561 gatgcaaacg ctgctggacg gcgtgcgtgc gcgtttgccg ctggcagcgg atgcagaaat
    19621 tactatggaa gcgaaccctg gaacggtaga agccgatcgc tttgtcgatt atcagcgtgc
    19681 tggtgtgaac cgcatctcta ttggtgtgca gagttttagc gaagaaaagc tgaaacgact
    19741 tgggcgtatt catggcccgc aagaagcgaa acgcgcggca aagctggcga gcgggctggg
    19801 gctgcgtagc tttaacctcg atttgatgca tggactaccg gatcaatcac tggaagaggc
    19861 gcttggcgat ctacgccagg ccattgaact gaatccgccg catctttcct ggtatcaact
    19921 gaccatcgaa cccaatacgc tgtttggttc gcgaccaccg gtgctgccgg acgatgacgc
    19981 gctgtgggat attttcgaac aggggcatca gttattaacc gcagcgggtt atcagcaata
    20041 tgaaacttcc gcttacgcca aacccggtta tcagtgccag cacaatctca actactggcg
    20101 atttggtgac tacatcggta ttggctgcgg cgcgcacggc aaagtcacct tcccggatgg
    20161 gcgcattctg cgtaccacta aaacgcgtca tcctcgtggt tttatgcaag gaaggtatct
    20221 ggaaagccag cgtgatgtcg aagccgcaga taagccgttt gagttcttta tgaatcgctt
    20281 ccgtctgctg gaggccgcgc cgcgcgtgga gtttattgcg tataccgggc tttgcgaaga
    20341 tgtgattcgc ccacagttag acgaggcgat tgcccagggt tatctcaccg aatgtgcgga
    20401 ttactggcag ataaccgagc acggtaaatt attcttaaac tcacttcttg aattattcct
    20461 cgctgagtaa tcattaatgc gcctgcgggc gcattaatca cttcttttcc ccccgttaat
    20521 taacttcggc aaaatatatt tcttactgaa taaacatcga tgatatttat tagatata
//
LOCUS       DAEEKQ010000065        20323 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.065, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000065 DAEEKQ010000000
VERSION     DAEEKQ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.065"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..251)
                     /locus_tag="KLK57_002242"
     CDS             complement(<1..251)
                     /locus_tag="KLK57_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HBE0892605.1"
                     /translation="MAQVAIFKQIFDKVRNNLNYHWFYSELKRHNVSHYIYYLATENI
                     HLVLENDNTVLIKGQGKVVNVRFSKNKCLIEATLKGFKSG"
     gene            complement(309..542)
                     /locus_tag="KLK57_002243"
     CDS             complement(309..542)
                     /locus_tag="KLK57_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HBE0892606.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALVQKGSGNFSAWVIEA
                     CRRRLCSEKRVSPEANKEKSDITELLRKQVRPD"
     gene            931..1476
                     /locus_tag="KLK57_002244"
     CDS             931..1476
                     /locus_tag="KLK57_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HBE0892607.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIRWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            1451..3376
                     /locus_tag="KLK57_002245"
     CDS             1451..3376
                     /locus_tag="KLK57_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HBE0892608.1"
                     /translation="MNISNSQVNRLRHFVRAGLRSLFRPEPQTAVEWADANYYLPKES
                     AYQEGRWETLPFQRAIMNAMGSDYIREVNVVKSARVGYSKMLLGVYAYFIEHKQRNTL
                     IWLPTDGDAENFMKTHVEPTIRDIPSLLALAPWYGKKHRDNTLTMKRFSNGRGFWCLG
                     GKAAKNYREKSVDVAGYDELAAFDEDIEQEGSPTFLGDKRIEGSVWPKSIRGSTPKVR
                     GTCQIERAASESPHFMRFHVACPHCGEEQYLKFGDKETPFGLKWTPDDPSSVFYLCEH
                     NACVIRQQELDFTDARYICEKTGIWTRDGILWFSSSGEEIEPPDSVTFHIWTAYSPFT
                     TWVQIVKDWMKTKGDTGKRKTFVNTTLGETWEAKIGERPDAEVMAERKEHYSAPVPDR
                     VAYLTAGIDSQRDRYEMRVWGWGPGEESWLIDRQIIMGRHDDEQTLLRVDEAINKTYT
                     RRNGAEMSISRICWDIGGIDPTIVYERSKKHGLFRVIPIKGASVYGKPVASMPRKRNK
                     NGVYLTEIGTDTAKEQIYNRFTLTPEGDEPLPGAVHFPNNPDIFDLTEAQQLTAEEQV
                     EKWVDGRKKILWDSKKRRNEALDCFVYALAALRISISRWQLDLSALLASLQEEDGAAT
                     NKKTLADYARALSGEDE"
     gene            3373..3579
                     /locus_tag="KLK57_002246"
     CDS             3373..3579
                     /locus_tag="KLK57_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gpW family protein"
                     /protein_id="HBE0892609.1"
                     /translation="MTRQEELAAARAALHDLMTGKRVATVQKDGRRVEFTATSVSDLK
                     KYIAELEVQTGMTQRRRGPAGFYV"
     gene            3576..5177
                     /locus_tag="KLK57_002247"
     CDS             3576..5177
                     /locus_tag="KLK57_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HBE0892610.1"
                     /translation="MKTPTIPTLLGPDGMTSLREYAGYHGGGSGFGGQLRAWNPPGES
                     VDAALLPNFTRGNARADDLVRNNGYAANAIQLHQDHIVGSFFRLSHRPSWRYLGIGEE
                     DARAFSREVEAAWKEFAEDDCCCIDVERKRTFTMMIREGVAMHAFNGELFVQATWDTS
                     SSRLFRTQFRMVSPKRISNPNNTGDSRNCRAGVQINDSGAALGYYVSEDGYPGWMPQK
                     WTWIPRELPGGRASFIHVFEPVEDGQTRGANVFYSVMEQMKMLDTLQNTQLQSAIVKA
                     MYAATIESELDTQSAMDFILGANSKEQRERLTGWIGEIAAYYAAAPVRLGGAKVPHLM
                     PGDSLNLQTAQDTDNGYSVFEQSLLRYIAAGLGVSYEQLSRNYAQMSYSTARASANES
                     WAYFMGRRKFVASRQASQMFLCWLEEAIVRRVVTLPSKARFSFQEARSAWGNCDWIGS
                     GRMAIDGLKEVQEAVMLIEAGLSTYEKECAKRGDDYQEIFAQQVRETMERRAAGLKPP
                     AWAAAAFESGLRQSTEEEKSDSRAA"
     gene            5158..6477
                     /locus_tag="KLK57_002248"
     CDS             5158..6477
                     /locus_tag="KLK57_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HBE0892611.1"
                     /translation="MTAELRNLPHIASMAFNEPLMLEPAYARVFFCALAGQLGISCLT
                     DAVSGDSLTAGEAPAALALSGDDDGPRQARSYQVMNGIAVLPVSGTLVSRTRALQPYS
                     GMTGYNGIIARLQQAASDPMVDGILLDMDTPGGMVAGAFDCADIIARVRDIKPVWALA
                     NDMNCSAGQLLASAASRRLVTQTARTGSIGVMMAHSNYGAALEKQGVEITLIYSGSHK
                     VDGNPYSHLPDDVRETLQSRMDATRRMFAQKVSAYTGLSVQAVLDTEAAVYSGQEAID
                     AGLADELVNSTDAITVMRDALDARKSRLSGGRMTKETQSTTVSATASQADVTGVVQAT
                     EGENASAAQPDVNAQITAAVAAENSRIMGILNCEEAHGREEQARVLAETPGMTVETAR
                     RILAAAPQSAQTRSDTALDRLMQGAPAPLAAGNPASDAVNDLLNTPV"
     gene            6487..6819
                     /locus_tag="KLK57_002249"
     CDS             6487..6819
                     /locus_tag="KLK57_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HBE0892612.1"
                     /translation="MTSKETFTHYQPLGNSDPAHTATAPGGLSAKAPAMTPLMLDTST
                     RKLVAWDGTTDGAAVGILAVAADQTSTTLTFYKSGTFRYEDVLWPEAASDETKKRTVF
                     AGTAISIV"
     gene            6875..7900
                     /locus_tag="KLK57_002250"
     CDS             6875..7900
                     /locus_tag="KLK57_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HBE0892613.1"
                     /translation="MSMYTTAQLLAANEQKFKFDPLFLRLFFRESYPFTTEKVYLSQI
                     PGLVNMALYVSPIVSGEVIRSRGGSTSEFTPGYVKPKHEVNPQMTLRRLPDEDPQNLA
                     DPAYRRRRIIMQNMRDEELAIAQVEEMQAVSAVLKGKYTMTGEAFDPVEVDMGRSAAN
                     NITQSGVTEWSKRDKSTYDPTDDIEAYALNASGVVNIIVFDPKGWALFRSFKAVKEKL
                     DTRRGSHSELETAVKDLGKAVSYKGMYGDVAIVVYSGQYVENGVKKNFLPDNTMVLGN
                     TQARGLRTYGCIQDADAQREGINASARYPKNWVTTGDPAREFTMIQSAPLMLLADPDE
                     FVSVQLA"
     gene            7942..8340
                     /locus_tag="KLK57_002251"
     CDS             7942..8340
                     /locus_tag="KLK57_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HBE0892614.1"
                     /translation="MTKDELIARLQELGEQLNRDVSLTGTKEELALRVAELEEELDDT
                     DETAGQDTPLSRENVLTGHENEVVSAQPDTVIQDTAELVTVVALVTLHTDALHATRDK
                     PVAFVLPGTAFRVSAGVAAEMTERGLARMQ"
     gene            8352..8705
                     /locus_tag="KLK57_002252"
     CDS             8352..8705
                     /locus_tag="KLK57_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail joining protein"
                     /protein_id="HBE0892615.1"
                     /translation="MADFDNLFDAAIARADETIRGYMGTSATMTSGEQSGAVIRGVFD
                     DPENISYAGQGVRVEGSSPSLFVRTDEVRQLRRGDTLTIGEENFWVDRVSPDDGGSCH
                     LWLGRGVPPAVNRRR"
     gene            8717..9295
                     /locus_tag="KLK57_002253"
     CDS             8717..9295
                     /locus_tag="KLK57_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0892616.1"
                     /translation="MAIKGLEQAVENLSRISKTAVPGAAAMAINRVASSAISQSASQV
                     ARETKVRRKLVKERARLKRATVKNPQARIKVNRGDLPVIKLGNARVVLSRRRRHKKGQ
                     RSSLKGGGSVLVVGNRRIPGAFIQQLKNGRWHVMQRVAGKNRYPIDVVKIPMAVPLTT
                     AFKQNIERIRRERLPKELGYALQHQLRMVIKR"
     gene            9292..9687
                     /locus_tag="KLK57_002254"
     CDS             9292..9687
                     /locus_tag="KLK57_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0892617.1"
                     /translation="MKHTELRAAVLDALEKHDTGATLFDGRPAVFDEEDFPAIAVYLT
                     GAEYTGEELDSDTWQAELHIEVFLPAQVPDSELDSWMESRIYPVMSDIPALSDLITSM
                     VASGYDYRRDDDAGLWSSADLTYVIIYEM"
     gene            9695..10435
                     /locus_tag="KLK57_002255"
     CDS             9695..10435
                     /locus_tag="KLK57_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0892618.1"
                     /translation="MPVPNPTMPVKGAGTTLWVYKGSGDPYANPLSDVDWSRLAKVKD
                     LTPGELTAESYDDSYLDDEDADWTATGQGQKSAGDTSFTLAWMPGEQGQQALLAWFNE
                     GDTRAYKIRFPNGTVDVFRGWVSSIGKAVTAKEVITRTVKVTNVGRPSMAEDRSTVTA
                     TTGMTVTPASASVVKGQSTTLTVAFQPDGATDKSFRAVSADKTKATVSVSGMTITVKG
                     VAAGKVNIPVVSGNGEFAAVAEINVTAS"
     gene            10451..10873
                     /locus_tag="KLK57_002256"
     CDS             10451..10873
                     /locus_tag="KLK57_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HBE0892619.1"
                     /translation="MFLKTESFEHNGVTVTLSELSALQRIEHLDLMKRQAEQAESDSN
                     RKFTVEDAIRTGAFVVAMSLWHNHPKKTQMPSMNEAVKQIEQEVLTTWPTEAISHAEN
                     VVYRLSGMYEFVVNDAPEQAEDAGPAEPVSAGKCSTVS"
     gene            10855..11289
                     /locus_tag="KLK57_002257"
     CDS             10855..11289
                     /locus_tag="KLK57_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HBE0892620.1"
                     /translation="MFDGELSFALKLAREMGRPDWRAMLAGMSSTEYADWHRFYSTHY
                     FHDVLLDMHFSGLTYTVLSLFFSDPEMHPLDFSLLNRREADEEPEDDVLMQKAAGLAG
                     GVRFGPDGNEVIPASPDVADMTEDDVMLMTVSEGIAGGVRYG"
     gene            11282..13843
                     /locus_tag="KLK57_002258"
     CDS             11282..13843
                     /locus_tag="KLK57_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBE0892621.1"
                     /translation="MAEPVGDLVVDLSLDAARFDEQMARVRRHFSGTESDAKKTAAVV
                     EQSLSRQALAAQKAGISVGQYKAAMRMLPAQFTDVATQLAGGQNPWLILLQQGGQVKD
                     SFGGMIPMFRGLAGAITLPMVGATSLAVATGALAYAWYQGNSTLSDFNKTLVLSGNQA
                     GLTADRMLVLSRAGQAAGLTFNQTSESLSALVKAGVSGEAQIASISQSVARFSYASGV
                     EVDKVAEAFGKLTTDPTSGLTAMARQFHNVTAEQIAYVAQLQRSGDEAGALQAANEAA
                     TKGFDDQTRRLKENMGTLETWADRTARAFKSMWDAVLDIGRPDTAQEMLIKAEAAFKK
                     ADDIWNLRKDDYFVNDEARARYWDDREKKRLERDAAQKRVDQQRQQDKNAQQQSDTEA
                     SRLKYTEEAQKAYERLQTPLEKYTARQEELNKALKDGKILQADYNTLMAAAKKDYEAT
                     LKKPKQSGVKVYAGDRQEDSAHAALLTLQAELRTLEKHAGANEKISQQRRDLWKAESQ
                     FAVLEEAAQRRQLSAQEKSLLAHKDETLEYKRQLAALGDKVTYQEHLNALAQQADKFA
                     QQQRAKRAAIDAKNRGLTDRQAAREATEQRLKEQYGDNPLALNNVMSEQKKTWAAEDQ
                     LRGSWMAGLKSGWSEWEESATDSMSQVKSAATQTFDGIAQNMAAMLTGSEQNWRSFTR
                     SVLSMMTEILLKQAMVGIVGSIGSAIGGAVGGGASASGGTAIQAAAVKFHFATGGFTG
                     TGGKYEPAGIVHRGEFVFTKEATSRIGVGNLYRLMRGYAEGGYVGGAGSPAQMRRAEG
                     INFNQNNHVVIQNDGTNGLPGPQMMKAVYDMARKGARDEIQTQMRDGGLFSGGGR"
     gene            13840..14169
                     /locus_tag="KLK57_002259"
     CDS             13840..14169
                     /locus_tag="KLK57_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0892622.1"
                     /translation="MKTFRWKVKPGMDVASAPSVRKVRFGDGYSQRAPAGLNANLKTY
                     SVTLSVPREEAMVLESFLEEHGGWKAFLWTPPYEWRQIKVTCAKWSSRVSMLRVEFSA
                     EFEQVVN"
     gene            14169..14867
                     /locus_tag="KLK57_002260"
     CDS             14169..14867
                     /locus_tag="KLK57_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBE0892623.1"
                     /translation="MQDIRQETLNECTRAEQSASVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQPYPIQGSGFELNGKGTSTRPTLMVSNLYGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLSTPTETDG
                     AVFPGRIMLANTCTWTYRGDECGYHGPAVADEYDQPTSDITKDKCSKCLSGCKFRNNV
                     GNFGGFLSINKLSQ"
     gene            14873..15616
                     /locus_tag="KLK57_002261"
     CDS             14873..15616
                     /locus_tag="KLK57_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0892624.1"
                     /translation="MTETESAILAHARRCAPAESCGFVVRTPEGERYFPCVNISGEPE
                     AYFRMSPEDWLQAEMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHREDDWWRHGQNLYLDNLEAT
                     GLYQVPLSAAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWHASAFTGICNDLAAASTFV"
     gene            15553..16185
                     /locus_tag="KLK57_002262"
     CDS             15553..16185
                     /locus_tag="KLK57_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBE0892625.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRAMATQLPAFRQKLSD
                     GWYQVRIAGQDVSTSGLTAQLHEALPDGAVIHIVPRVAGAKSGGVFQIVLGAAAIAGS
                     FFTAGATLAAWGAAIGAGGMTGILFSLGASMVLGGVAQMLAPKARTPRTQTTDNGKQN
                     TYFSSLDNMVAQGNVMPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            16246..19743
                     /locus_tag="KLK57_002263"
     CDS             16246..19743
                     /locus_tag="KLK57_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBE0892626.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVEGPVDGLKS
                     VLLNSTPVLDSEGNTNISGVTVVFRAGEQEQSPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRFTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVDNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGSQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGSTEPRITCNAWLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRQSDKVWAYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGIRTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGQGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     HQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDQSGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVTADDGSERLVSTARTTETTYRFTQ
                     LALGNYRLTVRAVNAWGQQGDPASVSFRIAAPAALSRIELTPGYFQITATPHLAVYDP
                     TVQFEFWFSEKRIADIRQVETTARYLGTALYWIAASINIKPGHDYYFYIRSVNTVGKS
                     AFVEAVGQPSDDASGYLDFFKGEIGKTHLAQELWTQIDNGQLAPDLAEIRTSITDVSN
                     EITQTVNKKLEDQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMQDGRLYIAGIGAGIEN
                     TPDGMQSQVLLAADRIAMVNPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     SAFSLTPDGKLTAKNADISGSVNANAGTLNNVTVNENCTIKGMLEATQVRGDFVKAVS
                     KSFPKQAGTWGNTETPNGTVTVTISDDHNFDRQIIIPPIIFNGIAYDDPGSGNNPGGT
                     RYTGYGFEVRKNGVLIASRETKGAIPGSYSAVIDMPSGGGSVTLEFKIFQKGNQGAGN
                     ITDCTVIVTKKAASGISIR"
     gene            19814..20284
                     /locus_tag="KLK57_002264"
                     /pseudo
     CDS             19814..20284
                     /locus_tag="KLK57_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309676.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
BASE COUNT         4670 a         5055 c         6293 g         4305 t
ORIGIN      
        1 cctgatttga atccttttaa ggtggcttct ataaggcatt tattttttga aaatcttaca
       61 tttacaacct taccctgtcc ttttattaaa accgtattat cgttttcaag aacaagatga
      121 atattctctg tggctaaata gtaaatgtaa tgtgagacat tgtgacgttt tagttcagaa
      181 taaaaccagt gatagtttaa attatttcgc actttatcga atatttgttt aaaaatggca
      241 acctgagcca ttgtagtacc ttccatgtga tatgaggggg cgtagtctgc acgattatct
      301 aaattgcttc aatctggtct aacctgtttt ctgagcaatt cagtaatgtc actcttttct
      361 ttgtttgctt caggcgaaac tcttttttct gagcacagtc tccggcggca ggcttcaatg
      421 acccaggctg agaaattccc ggaccctttt tgaacaagag cgatgttaat ttgttcaatc
      481 atttggttag gaaagcggat gttgcgggtt gttgttctgc gggttctgtt cttcgttgac
      541 atgaggttgc cctgtattca gtgtcgctga tttgtattgt ctgaagttgt ttttacgtta
      601 agttgatgca gatcaattaa tacgatacct gcgtcataat tgattatttg acgtggtttg
      661 atggcgtaga tgcacgttgt gacatgtaga tgataattat tatcattttg cgggtccttt
      721 ccggcgatcc gacaggttac ggggcggaag gcgcgcgggt tttcgctatt tatgacaatt
      781 ttccggttta aggcgtttcc gttcttcttc gccgtaacct aatgttttta tttaaaacac
      841 cccctgaaaa gaaaggaaac gacaggtgct gaaaacgggc tttttggcct ctgtcgtttc
      901 ctttctctgt ttttgtccgt ggaatgaaca atggaagtca acaaaaagca gctggctgac
      961 attttcggtg cgagtatccg taccattcag aactggcagg aacagggaat gcccgttctg
     1021 cgaggcggtg gcaagggtaa tgaggtgctt tatgactctg ccgccgttat aagatggtat
     1081 gccgaaaggg atgctgaaat tgagaacgaa aagctgcgcc gggaagttga agaactgcgg
     1141 caggccagcg agacagatct ccagccaggg actattgagt acgaacgcca tcgacttacg
     1201 cgtgcgcagg ccgacgcaca ggagctgaaa aatgccagag actccgctga agtggtggaa
     1261 accgcattct gtactttcgt gctgtcgcgg atcgcaggtg aaattgccag tattctcgac
     1321 gggatccccc tgtcggtgca gcggcgtttt ccggaactgg aaaaccgaca tgttgatttc
     1381 ctgaaacggg atatcatcaa agccatgaac aaagcagccg cgctggatga actgataccg
     1441 gggttgctga gtgaatatat cgaacagtca ggttaacagg ctgcggcatt ttgtccgcgc
     1501 cgggcttcgc tcactgttca ggccggagcc acagaccgcc gttgaatggg cggatgctaa
     1561 ttactatctc ccgaaagaat ccgcatacca ggaagggcgc tgggaaacac tgccctttca
     1621 gcgggccatc atgaatgcga tgggcagcga ctacatccgt gaggtgaatg tggtgaagtc
     1681 tgcccgtgtc ggttattcca aaatgctgct gggtgtttat gcctacttta tagagcataa
     1741 gcagcgcaac acacttatct ggttgccgac ggatggtgat gccgagaact ttatgaaaac
     1801 ccacgttgag ccgaccatcc gcgatattcc gtcgctgctg gcgctggctc cgtggtatgg
     1861 caaaaagcac cgggataaca cgctcactat gaagcgtttt tccaatggtc gtggcttctg
     1921 gtgcctgggc ggtaaagcgg caaaaaacta ccgtgaaaag tcggtggatg tggcgggtta
     1981 tgatgaactt gctgcctttg atgaggatat tgaacaggaa ggctctccga cgttccttgg
     2041 cgacaaacgt attgaaggct cggtctggcc aaagtccatc cgtggctcca cgcccaaagt
     2101 gagaggcacc tgccagattg agcgtgcagc cagtgaatcc ccgcatttta tgcgttttca
     2161 tgttgcctgc ccgcactgcg gggaggagca gtatcttaaa tttggcgaca aagagacgcc
     2221 gtttggcctc aaatggacgc cggatgaccc ctccagcgtg ttttatctct gcgagcataa
     2281 tgcctgcgtc atccgccagc aggagctgga ctttactgat gcccgttata tctgcgaaaa
     2341 gaccgggatc tggacccgtg atggcattct ctggttttcg tcatccggtg aagagattga
     2401 gccacctgac agtgtgacct ttcacatctg gacagcgtac agcccgttca ccacctgggt
     2461 gcagattgtc aaagactgga tgaaaacgaa aggggatacg ggaaaacgta aaaccttcgt
     2521 aaacaccacg ctcggtgaga cgtgggaggc gaaaattggc gaacgtccgg atgctgaagt
     2581 gatggcagag cggaaagagc attattcagc gcccgttcct gaccgtgtgg cttacctgac
     2641 cgccggtatc gactcccagc gggatcgcta cgaaatgcgc gtatggggat gggggccggg
     2701 tgaggaaagc tggctgattg atcggcagat tattatgggc cgccacgacg atgaacagac
     2761 gctgctgcgt gtggatgagg ccatcaataa aacctatacc cgccggaatg gtgcagaaat
     2821 gtcgatatcc cgtatctgct gggatattgg cgggattgac ccgaccattg tgtatgaacg
     2881 ctcgaaaaag catgggctgt tccgggtgat ccccattaaa ggggcatccg tctacggtaa
     2941 gccggtggcc agcatgcctc gtaagcgaaa caaaaacggg gtttacctta ccgaaatcgg
     3001 tacggatacc gcgaaagagc agatttataa ccgcttcaca ctgacgccgg aaggggatga
     3061 accgcttccc ggtgccgttc acttcccgaa taacccggat atttttgatc tgaccgaagc
     3121 gcagcagctg actgctgaag agcaggtcga aaaatgggtg gatggcagga aaaaaatact
     3181 gtgggacagc aaaaagcgac gcaatgaggc gctcgactgc ttcgtttatg cgctggcggc
     3241 gctgcgcatc agtatttccc gctggcagct ggatctcagt gcactgctgg cgagcctgca
     3301 ggaagaggat ggtgcagcaa ccaacaagaa aacactggca gattacgccc gtgccttatc
     3361 cggagaggat gaatgacgcg acaggaagaa cttgccgctg cccgtgcggc actgcatgac
     3421 ctgatgacag gtaaacgggt ggcaacggta cagaaagacg gacggcgagt ggagtttacg
     3481 gccacttccg tgtctgacct gaaaaaatac attgcggagc tggaggtgca gaccggcatg
     3541 acacagcgac gcaggggacc tgcaggattt tatgtatgaa aacgcccacc attcccaccc
     3601 ttctgggacc ggacggcatg acatcgctgc gtgaatatgc cggttatcac ggcggtggca
     3661 gcggatttgg tgggcagttg cgggcgtgga acccaccggg tgaaagtgtg gatgcagccc
     3721 tgctgcccaa ctttacccgt ggcaatgccc gcgcagacga tctggtacgc aataacggct
     3781 atgctgccaa cgccatccag ctgcatcagg atcatatcgt cgggtctttt ttccggctca
     3841 gtcatcgccc aagctggcgc tatctgggca tcggggagga agacgcccgt gccttttccc
     3901 gcgaggttga agcggcatgg aaagagtttg ccgaggatga ctgctgctgc attgacgttg
     3961 agcgaaaacg cacgtttacc atgatgattc gggaaggtgt ggccatgcac gcctttaacg
     4021 gtgaactgtt cgttcaggcc acctgggata ccagttcgtc gcggcttttc cggacacagt
     4081 tccggatggt cagcccgaag cgcatcagca acccgaacaa taccggcgac agccggaact
     4141 gccgtgccgg tgtgcagatt aatgacagcg gtgcggcgct gggatattac gtcagcgagg
     4201 acggctatcc tggctggatg ccgcagaaat ggacatggat accccgtgag ttacccggcg
     4261 ggcgcgcctc gttcattcac gtttttgaac ccgtggagga cgggcagacc cgcggtgcaa
     4321 atgtgtttta cagcgtaatg gagcagatga agatgctcga cacgctgcag aacacgcagc
     4381 tgcagagcgc cattgtgaag gcgatgtatg ccgccaccat tgaaagtgag ctggatacgc
     4441 agtcagcgat ggattttatt ctgggcgcga acagtaagga gcagcgggaa aggctgacgg
     4501 gctggattgg tgaaattgcc gcgtattacg ccgcagcacc ggtccgtctg ggaggcgcaa
     4561 aagtgccgca cctgatgccg ggggactcac tgaacctgca gacggctcag gacacggata
     4621 acggctactc cgtgtttgag cagtcactgt tgcggtatat cgctgccggg ctgggtgtct
     4681 cgtatgagca actttcccgg aattacgccc agatgagcta ctccacggca cgggccagtg
     4741 cgaacgagtc gtgggcgtac tttatggggc ggcgaaaatt cgtcgcatcc cgtcaggcga
     4801 gccagatgtt tctgtgctgg ctggaagagg ccatcgttcg ccgcgtggtg acgttacctt
     4861 caaaagcgcg cttcagcttt caggaagccc gcagtgcctg ggggaactgc gactggatag
     4921 gctccggtcg tatggccatc gatggtctga aagaagttca ggaagcggtg atgctgatag
     4981 aagccggact gagcacctac gagaaagagt gcgcgaaacg cggtgacgac tatcaggaaa
     5041 tttttgccca gcaggtccgt gaaacgatgg agcgccgcgc agctggtctt aaaccgcccg
     5101 cctgggcggc tgcggcattt gaatccgggc tgcgacaatc aacagaggag gagaagagtg
     5161 acagcagagc tgcgtaatct cccgcatatt gccagcatgg cttttaatga gccgctgatg
     5221 cttgaacccg cctatgcgcg ggttttcttt tgtgcgcttg caggccagct tgggatcagc
     5281 tgcctgacgg atgcagtatc cggcgacagc ctgactgccg gagaggcacc cgcggcgctg
     5341 gcgttatccg gtgatgatga cggaccacga caggcccgca gttatcaggt aatgaacggc
     5401 atcgccgtgc tgccggtgtc cggcacgctg gtcagccgga cgcgggcgct gcagccgtac
     5461 tcggggatga ccggttacaa cggcattatc gcccgtctgc aacaggctgc cagcgacccg
     5521 atggtggacg gcattctgct cgatatggac acaccgggcg gaatggtggc gggagcattt
     5581 gactgcgctg acatcatcgc ccgtgtgcgt gacataaagc cggtatgggc gctggccaat
     5641 gacatgaact gcagcgcagg tcagctgctt gccagtgccg cctcccggcg tctggtcacg
     5701 cagaccgccc ggacaggctc catcggcgtc atgatggctc acagtaatta cggcgctgca
     5761 ctggagaaac agggcgtgga aatcacgctg atttacagcg gcagccataa ggtggatggc
     5821 aacccctaca gccatctacc ggatgatgtc cgggaaacat tgcagtcccg gatggatgca
     5881 acccgccgga tgtttgcaca gaaggtgtcg gcatataccg gcctgtccgt gcaggctgtg
     5941 ctggataccg aggctgcagt gtacagcggt caggaggcca ttgatgccgg actggctgat
     6001 gaacttgtta acagtaccga tgcgatcacc gtcatgcgtg atgcactgga tgcacgtaaa
     6061 tcccgtctct caggagggcg aatgaccaaa gagactcaat caacaactgt ttcagccact
     6121 gcttcgcagg ctgacgttac tggcgtggtg caagcgacgg agggcgagaa cgccagcgcg
     6181 gcgcagccgg acgtgaacgc gcagatcacc gcagcggttg cggcagaaaa cagtcgcatt
     6241 atggggatcc tcaactgtga ggaggctcac ggacgcgaag aacaggcacg cgtgctggcc
     6301 gaaacccccg gtatgaccgt ggaaacggcc cgccgtattc tggccgcagc accacagagt
     6361 gcacagacgc gcagtgacac tgcgctggat cgtctgatgc agggggcacc ggcaccgctg
     6421 gctgcaggta acccggcatc tgatgccgtt aacgatttgc tgaacacacc agtgtaaggg
     6481 atgtttatga cgagcaaaga aacctttacc cattaccagc cgctgggcaa tagtgacccg
     6541 gctcataccg caaccgcgcc cggcggattg agtgcgaaag cgcctgcaat gaccccgctg
     6601 atgctggaca cctccacccg taagctggtt gcgtgggatg gcaccaccga cggtgctgcc
     6661 gttggcattc ttgcggttgc tgctgaccag accagcacca cactgacgtt ctacaagtcc
     6721 ggcacgttcc gttatgagga tgtgctctgg ccggaggctg ccagcgacga gacgaaaaaa
     6781 cggaccgtgt ttgccggaac ggcaatcagc atcgtttaac tttacccttc atcactaaag
     6841 gccgcctgtg tggctttttt tacgggattt ttttatgtcg atgtacacaa ccgcccagct
     6901 gctggcggca aatgagcaga aatttaagtt tgatccgctg tttctgcgtc tctttttccg
     6961 tgagagctat cccttcacta cggagaaagt ctatctctca caaattccgg gactggtaaa
     7021 catggcgctg tacgtttcgc cgattgtttc cggtgaggtt atccgttccc gtggcggctc
     7081 cacctctgaa tttacgccgg gatatgtcaa acccaagcat gaggtgaatc cgcagatgac
     7141 cctgcgtcgc ctgccggatg aagatccgca gaatctggcg gacccggctt accgccgccg
     7201 tcgcatcatc atgcagaaca tgcgtgacga agagctggcc attgctcagg tcgaagagat
     7261 gcaggcagtt tctgccgtgc tcaagggcaa atacaccatg accggtgaag ccttcgatcc
     7321 ggttgaggtg gatatgggcc gcagtgcggc gaacaacatc acacagtccg gcgtcacgga
     7381 gtggagcaag cgtgacaagt ccacgtatga cccgaccgac gatatcgaag cctacgcgct
     7441 gaacgccagc ggtgtggtga atatcatcgt gtttgacccg aaaggctggg cgctgttccg
     7501 ttccttcaaa gccgtcaagg agaagctgga tacccgtcgc ggctctcatt ccgagctgga
     7561 gacagcggta aaagacctgg gcaaagcggt gtcctataag ggaatgtatg gcgatgtggc
     7621 catcgtcgtg tattccggac agtacgtgga aaacggcgtc aaaaagaact tcctgccgga
     7681 caacacgatg gtgctgggga acactcaggc tcgcggtctg cgcacctatg gctgcattca
     7741 ggatgcggac gcacagcgcg aaggcattaa cgcttctgcc cgttacccga aaaactgggt
     7801 gaccaccggc gatccggcgc gtgagttcac catgattcag tcagcaccgc tgatgctgct
     7861 ggctgatcct gatgagttcg tgtccgttca actggcgtaa tcatggccct tcggggccat
     7921 tttctctctg tggaggagtt catgacgaaa gatgaactga ttgcccgtct tcaggagctg
     7981 ggtgagcaat tgaaccgcga tgtcagtttg acgggaacga aagaagaact ggcgcttcgt
     8041 gtggcagagc tggaagaaga gcttgatgac acggatgaaa ctgccggtca ggacacccct
     8101 ctcagccggg aaaatgtgct gaccgggcat gaaaatgagg tggtatcagc gcagccggat
     8161 accgtgattc aggatacggc tgaactggtc acggtcgtgg cactggtgac gctgcatact
     8221 gatgcacttc acgccacgcg ggataaacct gtggcatttg tgctgccggg aacggcgttt
     8281 cgtgtctctg ccggtgtggc agccgaaatg acagagcgcg gcctggccag aatgcaataa
     8341 cgggaggcgc tgtggctgat ttcgataacc tgttcgatgc tgccattgcc cgcgccgatg
     8401 aaacgatacg cgggtacatg ggaacgtcag ccaccatgac atccggtgag cagtcaggtg
     8461 cggtgatacg tggtgttttt gatgaccctg aaaatatcag ctatgccgga cagggcgtgc
     8521 gcgttgaagg ctccagcccg tccctgtttg tccggactga tgaggtgcgg cagctgcggc
     8581 gtggagacac gctgaccatc ggtgaggaaa atttctgggt agatcgggtt tcgccggatg
     8641 atggcggaag ttgtcatctc tggcttggac ggggcgtacc gcctgccgtt aaccgtcgcc
     8701 gctgaaaggg ggatgtatgg ccataaaagg tcttgagcag gccgttgaaa acctcagccg
     8761 tatcagcaaa acggcggtgc ccggtgccgc cgcaatggcc attaaccgcg ttgcttcatc
     8821 cgcgatatcg cagtctgcgt cacaggttgc ccgtgagaca aaggtacgcc ggaaactggt
     8881 aaaggaaagg gccaggctga aaagggccac ggtcaaaaat ccgcaggcca gaatcaaagt
     8941 taaccggggg gatttgcccg taatcaagct gggtaatgcg cgggttgtcc tgtcccgccg
     9001 caggcgtcat aaaaaggggc agcgttcatc cctgaaaggt ggcggcagcg tgcttgtggt
     9061 gggtaaccgt cgtattcccg gcgcgtttat tcagcaactg aaaaacggcc ggtggcatgt
     9121 tatgcagcgt gtggccggga aaaaccgtta ccccattgat gtggtgaaaa tcccgatggc
     9181 ggtgccgctg accacggcgt ttaaacagaa tattgaacgg atacggcgtg aacgtcttcc
     9241 gaaagagctg ggctatgcgc tgcagcatca actgagaatg gtaataaagc gatgaaacat
     9301 actgaactcc gtgcagccgt actggatgca ctggagaagc atgacaccgg ggcgacgctt
     9361 tttgatggtc gccccgctgt ttttgatgag gaagattttc cggcaattgc cgtttatctc
     9421 accggcgctg aatacacggg cgaagagctg gacagcgata cctggcaggc ggagctgcat
     9481 attgaagttt tcctgcctgc tcaggtgccg gattcagagc tggattcgtg gatggagtcc
     9541 cggatttatc ctgtgatgag cgatatcccg gcactgtcag atttgatcac cagtatggtg
     9601 gccagcggct atgactaccg gcgcgacgat gatgcgggcc tgtggagttc agccgatctg
     9661 acttatgtca ttatctatga aatgtgagga cgctatgcct gtaccaaatc ctacaatgcc
     9721 ggtgaaaggt gccgggacca ccctgtgggt ttataagggg agcggtgacc cttatgcgaa
     9781 cccgctttca gacgttgact ggtcgcgtct ggcaaaagtt aaagacctga cgcccggcga
     9841 actgaccgct gagtcctatg acgacagcta tctcgatgat gaagatgcag actggactgc
     9901 gaccgggcag gggcagaaat ctgccggaga taccagcttc acgctggcgt ggatgcccgg
     9961 agagcagggg cagcaggcgc tgctggcgtg gtttaatgaa ggtgataccc gtgcctataa
    10021 aatccgcttc ccgaacggca cggtcgatgt gttccgtggc tgggtcagca gtatcggtaa
    10081 ggcggtgacg gcgaaggagg tgattacccg cacggtgaag gtcaccaatg tgggacgtcc
    10141 gtcgatggca gaagatcgca gcacggtgac ggcgacgacc ggcatgacgg tgacacccgc
    10201 cagtgcttcc gtagtgaaag ggcagagcac cacgctgacc gtggcattcc agccggatgg
    10261 cgcaaccgac aagagcttcc gtgcggtgtc tgcggataaa acaaaagcca ccgtgtcggt
    10321 cagtggtatg accatcaccg tgaaaggtgt tgctgcaggc aaggtcaaca ttccggtcgt
    10381 atctggtaat ggtgagtttg ctgcggttgc agaaatcaac gtcaccgcca gttaatccgg
    10441 agagtcagcg atgttcctga aaaccgaatc atttgaacat aacggtgtga ccgtcacgct
    10501 ttctgaactg tcagccctgc agcgtattga gcatctcgac ctgatgaaac ggcaggcaga
    10561 acaggcggag tcagacagca accggaagtt tactgtggaa gacgccatca gaaccggcgc
    10621 ttttgtggtt gcgatgtccc tgtggcataa ccatccgaag aagacgcaga tgccgtccat
    10681 gaatgaagcc gttaaacaga ttgagcagga agtgcttacc acctggccca cagaggcaat
    10741 ttctcatgct gaaaacgtgg tgtaccggct gtccggtatg tatgagtttg tggtgaatga
    10801 tgctcctgaa caggcagagg acgccgggcc tgcagagcct gtttctgcgg gaaagtgttc
    10861 gacggtgagc tgagttttgc cctgaaactg gcgcgtgaga tggggcgacc cgactggcgc
    10921 gccatgcttg ccgggatgtc atccacggag tatgccgact ggcaccgctt ttacagtacc
    10981 cattattttc atgatgttct gctggatatg cacttttccg ggctgacgta caccgtgctc
    11041 agcctgtttt tcagcgatcc ggagatgcat ccgctggatt tcagtctgct gaaccggcgc
    11101 gaggctgacg aagagcctga agatgatgtg ctgatgcaga aagcggcagg gcttgccgga
    11161 ggtgtccgct ttggcccgga cgggaatgaa gttatccccg cttccccgga tgtggcggac
    11221 atgacggagg atgacgtaat gctgatgaca gtatcagaag ggatcgcagg aggagtccgg
    11281 tatggctgaa ccggtaggcg atctggtcgt tgatttgagt ctggatgcgg ccagatttga
    11341 cgagcagatg gccagagtca ggcgtcattt ttccggtacg gaaagtgatg cgaaaaaaac
    11401 agcggcagtc gttgaacagt cgctgagccg acaggcgctg gctgcacaga aagcggggat
    11461 ttccgtcggg cagtataaag ccgccatgcg tatgctgcct gcacagttca ccgacgtggc
    11521 cacgcagctt gcaggggggc agaatccctg gctgatcctg ctgcaacagg gtggtcaggt
    11581 taaggactcc ttcggcggga tgatccccat gttcaggggg cttgccggcg cgatcaccct
    11641 gccgatggtg ggggccacct cgctggcggt ggcgaccggt gcgctggcgt atgcctggta
    11701 tcagggcaac tcaaccctgt ccgatttcaa caaaacgctg gtcctttccg gtaatcaggc
    11761 gggactgacg gcagatcgta tgctggtcct gtccagagcc gggcaggcgg cagggctgac
    11821 gtttaaccag accagcgagt cactcagcgc actggttaag gcgggggtaa gcggtgaggc
    11881 tcagattgcg tccatcagcc agagtgtggc gcgtttctcc tatgcatccg gcgtggaggt
    11941 ggacaaggtc gctgaagcct tcgggaagct gaccacagac ccgacgtcgg ggctgacggc
    12001 gatggcacgc cagttccata acgtgacggc ggagcagatt gcgtatgttg ctcagttgca
    12061 gcgttccggc gatgaagccg gggcattgca ggcggcgaac gaggccgcaa cgaaagggtt
    12121 tgatgaccag acccgccgcc tgaaagagaa catgggcacg ctggagacct gggcagacag
    12181 gactgcacgt gcattcaaat ccatgtggga tgcggtgctg gatattggtc gtcctgatac
    12241 cgcgcaggag atgctgatta aggcagaggc tgcgtttaag aaagcagacg acatctggaa
    12301 tctgcgcaag gatgattatt tcgttaacga tgaagcacgg gcgcgttact gggatgatcg
    12361 tgaaaaaaaa cgcctggagc gggatgcagc ccaaaagagg gtagatcaac agcgtcaaca
    12421 ggacaaaaat gcgcagcagc agagcgatac cgaagcgtca cggctgaaat ataccgaaga
    12481 ggcgcagaag gcttacgaac ggctgcagac gccgctggag aaatataccg cccgtcagga
    12541 agaactgaac aaggcactga aagacggaaa aatcctgcag gcagattaca acacgctgat
    12601 ggcggcggcg aaaaaggatt atgaagcgac gctgaaaaag ccgaaacagt ccggcgtgaa
    12661 ggtgtatgcg ggcgatcgtc aggaagacag tgctcatgcg gccctgctga cgcttcaggc
    12721 agaactccgg acgctggaga agcatgccgg agcgaatgag aaaatcagcc agcagcgccg
    12781 ggatttgtgg aaggcagaaa gtcagttcgc ggtactggag gaggcggcac aacgtcgcca
    12841 gctgtccgca caggagaaat ccctgctggc gcataaagac gagacgctgg agtacaaacg
    12901 ccagctggct gcacttggtg acaaggttac gtatcaggag cacctgaatg cgctggcgca
    12961 gcaggcggat aaattcgcac agcagcaacg ggcaaaacgg gcagccattg atgcgaaaaa
    13021 ccgggggctg actgaccggc aggcagcgcg ggaagccacg gaacagcgcc tgaaggaaca
    13081 gtatggcgat aatcctctgg cgctgaataa cgtcatgtca gagcagaaaa agacctgggc
    13141 ggctgaagac cagcttcgcg ggagctggat ggcaggcctg aagtccggct ggagtgagtg
    13201 ggaagagagc gccacggaca gtatgtcaca ggttaaaagt gcagccacgc agacctttga
    13261 tggtattgca cagaatatgg cggcgatgct gaccggcagt gaacagaact ggcgcagctt
    13321 cacccgctcc gtgctgtcca tgatgacaga aattctgctt aagcaggcaa tggtggggat
    13381 tgtcgggagt atcggcagcg ccattggcgg ggctgttggt ggcggcgcat ccgcgtcagg
    13441 tggtacagcc attcaggccg ctgcggtgaa attccatttt gcaaccgggg gatttacggg
    13501 aaccggcggc aaatatgagc cagcggggat tgttcaccgt ggtgaatttg tcttcacgaa
    13561 ggaggcaacc agccggattg gcgtggggaa tctctaccgg ctgatgcgcg gctatgcgga
    13621 aggtggttat gtgggcggtg ccggaagtcc ggcgcagatg cggcgggctg aaggcattaa
    13681 ttttaatcag aacaatcacg tggtgattca gaacgacggt acgaatggtc tgccaggtcc
    13741 acagatgatg aaggccgtgt atgacatggc ccgcaagggt gcccgtgatg aaattcagac
    13801 acagatgcgt gatggtggcc tgttctccgg aggtggacga tgaaaacctt ccgctggaaa
    13861 gtgaaacccg gtatggatgt ggcttcggcc ccttctgtaa gaaaggtgcg ctttggtgat
    13921 ggctattccc agcgagcgcc tgccgggctg aatgccaacc tgaaaacgta cagcgtgacg
    13981 ctttctgtcc cccgtgagga ggccatggta ctggagtcgt ttctggaaga gcacgggggc
    14041 tggaaagcct ttctgtggac gccgccttat gagtggcggc agataaaggt gacctgcgca
    14101 aaatggtcgt cgcgggtcag tatgctgcgt gttgagttca gcgcagagtt tgaacaggtg
    14161 gtgaactgat gcaggatatc cggcaggaaa cactgaatga atgcacccgt gcggagcagt
    14221 cggccagcgt ggtgctctgg gaaatcgatc tgacagaggt cggtggagaa cgttattttt
    14281 tctgtaatga gcagaacgaa aaaggtgagc cggtcacctg gcaggggcga cagtatcagc
    14341 cgtatcccat tcaggggagt ggttttgaac tgaatggcaa aggcaccagt acgcgcccca
    14401 cgctgatggt ttctaacctg tacggtatgg tcaccgggat ggcggaagat ctacagagtc
    14461 tggtcggcgg aacggtggtc cggcgtaagg tttacgcccg ttttctggat gcggtgaact
    14521 tcgtcaacgg aaacagtgac gccgatccgg agcaggaggt gatcagccgc tggcgcattg
    14581 agcagtgcag cgaactgagc gcggtgagtg cctcttttgt actgtccacg ccgacggaaa
    14641 cagatggcgc tgtttttccg ggacgtatca tgctggccaa cacctgcacc tggacctatc
    14701 gcggcgatga gtgcggttat cacggtccgg cggtcgcgga tgaatatgac cagccaacgt
    14761 ccgacatcac gaaggataaa tgcagcaaat gcctgagtgg ctgtaagttt cgcaataacg
    14821 tcggcaactt tggcggcttc ctttccatta acaaactttc gcagtaaatc ccatgacaga
    14881 gacagaatca gcgattctgg cgcacgcccg gcgatgtgct ccagcggagt cgtgcggctt
    14941 cgtggtaaga acgccggagg gggaaagata tttcccctgc gtgaatatct ccggtgagcc
    15001 ggaggcgtat ttccgtatgt cgccggaaga ctggctgcag gcagagatgc agggtgagat
    15061 tgtggcactg gtccacagcc accccggtgg tctgccctgg ctgagtgagg ccgaccggcg
    15121 gctgcaggtg cagagtgatt tgccgtggtg gctggtctgc cggggggcga ttcacaagtt
    15181 ccgctgtgtg ccacatctta ccgggcggcg ctttgagcac ggggtgacgg actgttacac
    15241 gctgttccgg gatgcttacc atctggcggg gattgagatg ccggattttc atcgtgagga
    15301 tgactggtgg cgtcacggtc agaatctcta tctggataat ctggaggcca cagggctgta
    15361 tcaggtgccg ttgtcagcgg cgcagccggg cgatgtgctg ctgtgctgtt ttggttcatc
    15421 ggtgccgaat catgccgcca tttactgtgg tgacggcgag ctgctgcacc atattcctga
    15481 acaactgagc aaacgagaga ggtataccga caaatggcag cgacgcacac actccctctg
    15541 gcgtcaccgg gcatggcacg catctgcctt tacggggatt tgcaacgatt tggccgccgc
    15601 atcgaccttc gtgtgaaaac gggtgccgaa gccatccggg cgatggccac acagctccct
    15661 gcgtttcgtc agaagctgag cgacggctgg tatcaggtgc gtattgccgg gcaggatgtt
    15721 agcacgtccg gattaacggc acagttacat gaggctctgc ctgacggcgc tgtgattcat
    15781 attgttccca gagtcgccgg ggccaagtca ggtggtgtat tccagattgt cctgggagca
    15841 gccgccattg ccggatcatt ctttaccgcc ggagccaccc ttgcagcatg gggggctgcc
    15901 attggtgccg gtggtatgac cggcattctg ttttctctcg gtgccagtat ggtgctcggc
    15961 ggtgtggctc agatgctggc accgaaagcc agaactcccc gcacacagac aacggataac
    16021 ggtaagcaga acacctattt ctcctcactg gataacatgg ttgcccaggg caatgttatg
    16081 cctgttctgt atggtgaaat gcgcgtgggg tcacgtgtgg tatctcagga gatcagcacg
    16141 gcagacgaag gggacggtgg tcaggttgtg gtgattggtc gctgatgcaa aatgttttat
    16201 gtgaaatcgc ctccgggcgg ttttatcgtt tatggagcat gacgaatggg taaaggcagc
    16261 agtaaggggc ataccccgcg cgaagcgaag gacaacctga agtccacgca gctgctgagt
    16321 gtgatcgatg ccatcagcga agggccggtt gaaggtccgg tggatggatt aaaaagcgtg
    16381 ctgctgaaca gtacgccggt gctggacagt gaggggaata ccaacatctc cggcgtcacg
    16441 gtggtgttcc gggcaggtga gcaggagcag tcaccgccgg agggatttga atcctccggc
    16501 tccgagacgg tgctgggtac ggaagtgaaa tatgacacgc cgatcacccg gaccatcacg
    16561 tcggcaaaca ttgaccgact gcgttttacc ttcggtgtac aggcactggt ggaaaccacc
    16621 tcaaagggag acaggaatcc gtctgaagtt cgcctgctgg ttcagataca gcgtaacggt
    16681 ggctgggtga cggaaaaaga catcaccatt aagggcaaaa ccacctcgca gtatctggcc
    16741 tcggtggtgg tggataacct gccgccgcgc ccgtttaata tccggatgcg cagaatgacg
    16801 ccggacagca ccacagacca gctgcagaac aaaacgctct ggtcgtcata caccgaaatc
    16861 atcgatgtga aacagtgcta cccgaacacg gcactggtcg gcgtacaggt ggattcggag
    16921 cagttcggca gccagcaggt gagccgtaat tatcatcttc gcgggcgcat tctgcaggtg
    16981 ccgtcgaact ataatccgca gacgcggcaa tacagcggta tctgggacgg aacgttaaaa
    17041 ccggcataca gcaacaacat ggcctggtgt ctgtgggata tgctgaccca tccgcgctat
    17101 ggcatgggga aacgtcttgg tgcggcagat gtggataaat gggcgctgta tgtcatcggc
    17161 cagtactgtg accagtcggt gccggacggc tttggcagca cggagccgcg catcacctgt
    17221 aatgcctggc tgaccacaca gcgcaaggcg tgggatgtgc tcagtgattt ctgctcggcg
    17281 atgcgctgta tgccggtatg gaacgggcag acgctgacgt tcgtgcagga ccgacagtcg
    17341 gataaggtgt gggcctataa ccgcagtaat gtggtgatgc cggatgatgg cgcgccgttc
    17401 cgctacagtt tcagcgccct gaaagaccgc cataatgccg ttgaggtgaa ctggattgac
    17461 ccggataacg gctgggagac ggcgacagag cttgtggagg atacgcaggc cattgtccgt
    17521 tacggtcgta acgtcacgaa gatggatgcc tttggctgta ccagccgggg gcaggcacac
    17581 cgcgccgggc tgtggctgat taaaacggaa ctgctggaga cgcagaccgt ggatttcagc
    17641 gtgggtgctg aagggcttcg tcatgtaccg ggcgatgtca ttgaaatctg cgatgatgac
    17701 tatgccggta tccgcaccgg cgggcgcgtg ctggcggtga acagccagac ccggacgctg
    17761 acgctcgacc gtgaaatcac gctgccatcc tccggcacca cgctgataag cctggttgac
    17821 gggcagggga atccggtcag cgtggaggtc cagtccgtca ccgacggcgt gaaggtaaaa
    17881 gtgagccgtg ttcctgacgg tgttgctgaa tacagcgtgt gggggctgaa gctgccgacg
    17941 ctgcaccagc gattgttccg ctgcgtgagt atccgtgaga acgacgacgg cacgtatgcc
    18001 atcaccgccg tgcagcatgt gccggaaaaa gaagccatcg tggataacgg ggcgcacttt
    18061 gacggcgacc agagcggcac ggtgaatggt gtcacgccgc cagcggtgca gcacctgact
    18121 gccgaagtca ccgcagacag cggggaatat caggtgctgg cgcgctggga cacgccgaag
    18181 gtggtgaagg gcgtgagctt cctgctccgt ctgaccgtaa cagcggatga cggcagtgag
    18241 cggctggtca gcacggcccg gacgacggaa accacttacc gcttcacaca actggcgctg
    18301 gggaactaca ggctgacagt ccgggcagta aatgcgtggg ggcagcaggg cgatccggcg
    18361 tcggtatcgt tccggattgc cgcaccggca gcgctgtcgc ggattgagct gacgccgggc
    18421 tattttcaga taaccgccac gccgcatctt gcggtttatg atccgacggt acagtttgag
    18481 ttctggttct cggaaaagcg gattgcggat atcaggcagg ttgaaaccac agcacgctat
    18541 cttggcacgg cgctgtactg gatagccgcc agtatcaata tcaaaccggg ccatgattat
    18601 tacttttata tccgcagtgt gaacaccgtt ggcaaatcgg cattcgtgga ggctgttggt
    18661 cagccgagtg atgatgcatc cggttatctg gattttttca aaggcgagat agggaaaacc
    18721 catctggctc aggagctgtg gacgcagatt gataacggtc agcttgcgcc tgacctggct
    18781 gaaatcagga cgtccattac ggatgtcagc aatgaaatca cgcagaccgt caataagaaa
    18841 ctggaagacc agagtgcggc aattcagcag atacagaagg ttcaggttga tacaaataat
    18901 aacctgaaca gcatgtgggc tgtgaagctg cagcagatgc aggacggacg cctttatatc
    18961 gcgggtattg gtgccggtat tgagaacacc cctgacggca tgcagagtca ggtgctgctg
    19021 gcggcggaca ggattgcgat ggttaatcct gcgaatggca acacaaaacc gatgtttgtt
    19081 ggtcagggcg atcagatatt catgaacgac gtgttcctga aacgcctgac ggcccccacc
    19141 attaccagcg gtggaaatcc atcggcattt tccctgactc cggacggaaa gctgaccgct
    19201 aaaaatgcgg atatcagtgg cagtgtgaat gcgaacgccg ggacgctcaa caatgtcacg
    19261 gtaaatgaaa actgtacgat taagggcatg ctggaggcga ctcaggtcag aggtgacttc
    19321 gttaaagctg tatccaaatc atttccgaaa caggctggta cgtggggtaa cacggaaaca
    19381 ccaaacggga cggttacagt caccataagc gatgatcata actttgaccg ccagattatt
    19441 attccgccca ttatttttaa cggtatagcg tatgacgatc cggggagcgg aaataaccca
    19501 ggaggcacgc gatacacggg ttatggtttt gaagttcgca aaaacggcgt attaatcgca
    19561 tccagagaaa ctaaaggtgc cattcccggt agttacagtg cagttattga tatgcctagt
    19621 ggtggtggta gcgtcactct ggagtttaag attttccaga aaggcaatca gggggcaggc
    19681 aatatcaccg actgtacggt gattgtgacc aaaaaagcgg cttccggcat cagtattcgt
    19741 tgaaatattt ataaccccaa taaatggcgt caggaatgac gcctttttta ttgcagaaaa
    19801 gcgagaggta attatgcgta aagtttgtgc agccattttg tccgcagcca tttgcctggc
    19861 cgtatccggt gcgcctgcat gggcgtctga acatcagtcc acgctgagcg cggggtatct
    19921 tcatgcccgg accaacgttc ccggcagtga tgatctgaac gggattaacg tgaaataccg
    19981 ttatgagttt acggatacgc tggggctggt gacgtcattc agctatgcag gagacaagaa
    20041 tcgccagctg acccgttaca gcgatacccg ctggcatgaa gattccgtgc gtaaccgctg
    20101 gttcagcgtg atggcggggc cgtctgtgcg cgtgaatgaa tggttcagcg cgtatgcgat
    20161 ggcgggtgtg gcttacagcc gtgtgtcgac tttttccggg gattatatcc gcgtaactga
    20221 caataagggg aaaacgcacg atgtgctgac cggaagtgat gacggtcgcc acagcaacac
    20281 gtctctggcg cactgacggt ttcatcgtgg gtgtcggtta taa
//
LOCUS       DAEEKQ010000066        20147 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.066, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000066 DAEEKQ010000000
VERSION     DAEEKQ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.066"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1277)
                     /locus_tag="KLK57_002265"
     CDS             complement(<1..1277)
                     /locus_tag="KLK57_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892627.1"
                     /translation="MEYFMKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDEIQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYM
                     STLDQIKRMQDVISNPKRLDGVPGQMNAVHIRFDSNSPVMADVQEEGKPQLWNHKQND
                     ALGLYLDLLIQAIDTGTINAEDWQKGDRLKSVALLIAYLDKANFYVMEDSGAWEEDAR
                     LNTSSVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYE
                     RITLQLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAG
                     PYGIKRYEKDNYQSANFWFNDIKTDTDQNSHAK"
     gene            complement(1470..1539)
                     /locus_tag="KLK57_002266"
     tRNA            complement(1470..1539)
                     /locus_tag="KLK57_002266"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1505..1507),aa:Gly,seq:ccc)
     gene            complement(1618..2373)
                     /locus_tag="KLK57_002267"
     CDS             complement(1618..2373)
                     /locus_tag="KLK57_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HBE0892628.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            2788..5085
                     /gene="xdhA"
                     /locus_tag="KLK57_002268"
     CDS             2788..5085
                     /gene="xdhA"
                     /locus_tag="KLK57_002268"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HBE0892629.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGRN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFAPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPSAFGCTFVD
                     LTVDIALCKVTINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            5096..5974
                     /gene="xdhB"
                     /locus_tag="KLK57_002269"
     CDS             5096..5974
                     /gene="xdhB"
                     /locus_tag="KLK57_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HBE0892630.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLADGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEAPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGSAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            5971..6450
                     /gene="xdhC"
                     /locus_tag="KLK57_002270"
     CDS             5971..6450
                     /gene="xdhC"
                     /locus_tag="KLK57_002270"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HBE0892631.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(6490..8268)
                     /locus_tag="KLK57_002271"
     CDS             complement(6490..8268)
                     /locus_tag="KLK57_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HBE0892632.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQFEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            8747..9934
                     /gene="ygeW"
                     /locus_tag="KLK57_002272"
     CDS             8747..9934
                     /gene="ygeW"
                     /locus_tag="KLK57_002272"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HBE0892633.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEERTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            9992..11188
                     /gene="dpaL"
                     /locus_tag="KLK57_002273"
                     /pseudo
     CDS             9992..11188
                     /gene="dpaL"
                     /locus_tag="KLK57_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
     gene            11246..12457
                     /locus_tag="KLK57_002274"
     CDS             11246..12457
                     /locus_tag="KLK57_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HBE0892634.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            12510..13894
                     /gene="hyuA"
                     /locus_tag="KLK57_002275"
                     /pseudo
     CDS             12510..13894
                     /gene="hyuA"
                     /locus_tag="KLK57_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
     gene            13942..14874
                     /gene="arcC"
                     /locus_tag="KLK57_002276"
     CDS             13942..14874
                     /gene="arcC"
                     /locus_tag="KLK57_002276"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBE0892635.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVIAVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERFIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(14915..16540)
                     /locus_tag="KLK57_002277"
     CDS             complement(14915..16540)
                     /locus_tag="KLK57_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HBE0892636.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EELDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILWVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMVDPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTDVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(16588..17358)
                     /gene="yqeC"
                     /locus_tag="KLK57_002278"
     CDS             complement(16588..17358)
                     /gene="yqeC"
                     /locus_tag="KLK57_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HBE0892637.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            17461..18039
                     /gene="mocA"
                     /locus_tag="KLK57_002279"
     CDS             17461..18039
                     /gene="mocA"
                     /locus_tag="KLK57_002279"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HBE0892638.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMFPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLMQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAQKRYTKI"
     gene            18361..>20147
                     /gene="ygfK"
                     /locus_tag="KLK57_002280"
     CDS             18361..>20147
                     /gene="ygfK"
                     /locus_tag="KLK57_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HBE0892639.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMLQPSDSGKSFIFNMSIGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRENLQALPARIPTS
                     MVQGVPLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMVLAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQ"
BASE COUNT         5224 a         5179 c         4765 g         4979 t
ORIGIN      
        1 cttttcgcat gtgaattttg atcggtatcg gttttaatat cattgaacca aaaatttgcc
       61 gactgataat tatccttctc gtatcttttt ataccatacg gacctgctag ttttttaact
      121 atttttagaa cctgctcttt tcttctggtg tttatctttg caagatttgc aggataaata
      181 acattaagta aagccgcatc ggcttctctg taatgtttat ctttttccag ataacctggg
      241 gattctcccc ctaaatcaag ctgtaacgtt attctttcat aacctttatc tatgaggtgg
      301 ttcaacctcg ttgttgaaag aggttcatcc agttcattgg ctttagcttc gcgtagtaaa
      361 tcagagacaa aaacagagtc tttcttcgag agtaagttcg acaaacgttc cagtccagag
      421 gtcaccagtg caacggaaga tgtgtttaat cgggcatctt cttcccatgc tccggaatct
      481 tccataacat agaaattggc tttatccaga taagcaatca gcagcgcaac cgattttaat
      541 cggtcacctt tttgccaatc ttcagcgtta attgtaccag tgtcgattgc ttgtataagg
      601 agatcaagat aaagacctaa cgcatcattt tgtttatggt tccacagctg cggcttgcct
      661 tcttcctgca catctgccat cacgggggag ttgctgtcaa atcggatatg cacagcattc
      721 atttgcccgg gaactccatc cagtcgtttg gggttgctaa tgacgtcctg catccgctta
      781 atctggtcga gcgtagacat ataatcccac agggtgagca gaaccttttt tgcggctaca
      841 ctatttccct gatctgatac taatgccata tagccccata aactatccct cagccagaca
      901 gcatcatagt gggtttctgc cgctgttttg tcatcttcga aagtagaagc aagaataaat
      961 gttccccagg gtttctccgt caacgttaac tttcgtgtgt tttctgttaa tgttaacaac
     1021 ccttgaattt cgtctttggt atagtggctc tttatttcat tttttaattt ggctgagtga
     1081 atcagaagac gataattagg gtacttatct ggatttaacg ataaataact tttatctgtt
     1141 gctgcccccg tggaattaag aatgttaaat acagattcac tcgtcacttc tgcccctgtc
     1201 gcctggcatg tgccgcacga aaatatacca gcgagcagaa gagttaatgc agatttccct
     1261 ttcataaaat attccatcaa tcgtttgtta aaagcgtaaa taaccactta tttaacaact
     1321 tgaaaataag gtgttttacc tgggttgtta caaaaggatt gcattgcgta aacgcttttt
     1381 atttacaaca aaatggggaa atattacggc gattgcgaaa agtctgaagc gattgaatta
     1441 agataatata gaaaatcaga tacagcaaaa gcggcgaagg gaatcgaacc ctcgtataga
     1501 gcttgggaag ctctcgttct accattgaac tacgcccgct tcgagatgcg taaggcatta
     1561 taaaccttac gctctcctta gcaagtgccg cgctgctgac tgctgattaa ttcgccatca
     1621 gcattttggc ttgctgccct gcggcggcaa gtaacgtagc ggatcaattg ccgttgcacg
     1681 gtaacgaatc tggaaatgca ggcgaacaga tgccgcatcc gtgctcccca tagtggcgat
     1741 tttttgccca gccttcacgc tttgcccatt atttaccagc atcgtgtcat tatgggcgta
     1801 agccgtaatg taatcttcac tgtgtttaat catgatgaga ttaccgtagc cacgcagctg
     1861 gttgcccaca tacaccacct ttcctgcacc cgcggcgtaa ataggtgtac cccgtggagc
     1921 tgagatatca atccctttat tgccgccatc tgctgtcgaa tacggcatga taactttccc
     1981 tgtcgttggc cataaccaac aacgttgccc tactggcggc caggatgatt tcggtaccgc
     2041 tgatgacggt gtaaccgatg cggttttggt cgttgatttg gcggttgatt tacgtgtact
     2101 actgctactt ttcgccccac ccagttttag tttctgacca acttcaatgg tgtaaggggg
     2161 ggaaatgccg ttcagtcgcg ccagttcttt tacgctggtt cccgtggtgc gcgaaatacg
     2221 atatagcgta tccccccgtt tcacggtgta aacggagccg gaatacgttc ctgtatcgga
     2281 tgatttgcta cccgaacagc ccgccaaaag cagtccaacc gataacaaca tcacgatacc
     2341 cagagatttt ttattcaggc gtcccgcact caaaatcttt cctcgctaaa aaaacaagac
     2401 gccttatcat agcagccagc ctcttgatac ccaatctctt gcttctggaa aaacgtggta
     2461 atagagaaag aggttaattt catttggcaa agggaagtat catcagcaaa atattcgctg
     2521 tagaggtatc gaacttcctg actttttacc ctatttccca tcctgatgcg taataatccc
     2581 aatttatcac attgatttta tcgccactca tattgatacg tatcactgcc cctattcaga
     2641 gtcctgtttc gtatcatatc tgtgtttttt taacgtcatc acacttccta cctcccctaa
     2701 cgcttatcgt cgtttctggc gtaaatcttg cctgcttaga ctaaatcttt gccataagaa
     2761 ccaaatgttc aaatcgaggg gatttctatg gaagcgcggg aagcaaccgc tacgggtgaa
     2821 tcatgcatgc gcgtcgatgc cattgctaag gtcaccgggc gggcacgata tactgacgat
     2881 tatgttatgg cgggcatgtg ttatgcgaaa tatgtacgta gccctatcgc acatggttat
     2941 gccgtaagta ttaatgatga acaagccaga agtttgccgg gcgtactggc gatttttacc
     3001 tgggaagatg tgcctgatat tccattcgct acagctgggc atgcctggac acttgacgaa
     3061 aacaagcgcg ataccgccga tcgcgcactg ctaactcgcc atgttcgtca tcatggtgac
     3121 gccgttgcca tcgtcgtggc ccgcgatgaa ctcacggcag aaaaagcggc gcaattggtc
     3181 agcattgagt ggcaagaatt acccgttatc accacgccag aagcggcgct ggcagaagac
     3241 gctgcaccaa tccataacgg tggcaattta ctgaaacaaa gcacgatgtc gacgggtaat
     3301 gtccaacaaa caatcgatgc cgccgactac caggtacagg ggcactatca gacccccgtt
     3361 attcaacatt gtcacatgga aagcgtaaca tcgctggcgt ggatggagga tgactcgcga
     3421 attaccatcg tttccagcac ccagatcccg cacattgttc gccgcgtggt tggtcaggcg
     3481 ctggatattc cctggtcatg cgtacgagtc atcaaaccgt ttgtcggtgg cggttttggt
     3541 aataaacagg atgtactgga agagccaatg gcagcattcc tgaccagcaa gcttggcggc
     3601 attccggtga aagtttccct tagccgtgaa gagtgtttcc tcgcaacccg tacccgccac
     3661 gcttttacca ttgacgggca aatgggcgtg aaccgcgacg gaacattgaa aggttatagt
     3721 ctggatgttc tgtctaacac cggcgcttat gcatctcacg ggcactccat cgcttctgcg
     3781 gggaggaata aagtcgctta cctttatcct cgttgtgcct acgcttacag ttcaaagacc
     3841 tgctatacca acctcccctc ggctggtgcg atgcgtggtt atggcgcgcc acaagtcgta
     3901 tttgccgttg agtctatgct tgatgacgcc gcgacagcgt taggtattga tcctgttgaa
     3961 attcgtttac gcaacgccgc ccgcgaagga gatgctaatc cgctcacggg caaacgtatt
     4021 tacagcgcag ggttgccgga gtgtcttgaa aaaggccgga aaatctttga atgggaaaaa
     4081 cgccgtgcag aatgccagaa ccagcaaggc aatttgcgcc gcggcgttgg cgtcgcctgt
     4141 tttagctata cctctaacac ctggcctgtc ggcgtagaaa tagcaggcgc gcgccttctg
     4201 atgaatcagg atggaaccat caacgtgcaa agcggcgcga cggaaatcgg tcagggtgcc
     4261 gacaccgtct tctcgcaaat ggtggcagaa accgtggggg ttccggtcag cgacgttcgc
     4321 gttatttcaa ctcaagatac cgacgttacg ccgttcgctc ccggcgcatt tgcctcacgc
     4381 cagagctatg ttgccgcgcc tgcgctgcgc agtgcggcac tattattaaa agagaaaatc
     4441 atcgctcacg ccgcagtcat gctacatcag tcagcgatga atctgaccct gataaaaggc
     4501 catatcgtgc tggttgaacg accggaagag ccgttaatgt cgttaaaaga tttggcgatg
     4561 gacgctttct accaccctga acgcggcggg cagctctctg ctgaaagctc catcaaaacc
     4621 accactaacc catcggcgtt tggctgtacc tttgttgatc tgacggtcga tattgcgctg
     4681 tgcaaagtca ccatcaaccg catcctcaac gttcatgatt cagggcatat tcttaatcca
     4741 ctgctggcag aaggtcaggt acacggcgga atgggaatgg gcattggctg ggcgctattt
     4801 gaagagatga tcatcgatgc taaaagcggc gtggtccgta accccaatct gctggattac
     4861 aaaatgccga ccatgccgga tctgccacaa ctggaaagcg cgttcgtcga aatcaatgag
     4921 ccgcaatccg catacggaca taagtcactg ggtgagccac caataattcc tgttgccgct
     4981 gctattcgta acgcggtgaa gatggctacc ggtgttgcaa tcaatacact gccgctgacg
     5041 ccaaaacggt tatatgaaga gttccatctg gcaggattga tttgaggata acatcatgtt
     5101 tgattttgct tcttaccatc gcgcagcaac ccttgccgat gccatcaacc tgctggctga
     5161 caacccgcag gccaaactgc tcgccgatgg cactgacgta ctgattcagc tccaccatca
     5221 caatgaccgt tatcgccata ttgttgatat tcataatctg gcggagctgc ggggaattac
     5281 gctggcggaa gatggctcgc tacgtatcgg ctctgcaacg acatttaccc agctaataga
     5341 agctcctata actcaacgtc atctcccggc gttatgtgct gcggccacgt ccattgctgg
     5401 accgcagatc cgtaacgtcg ctacctacgg tggaaatatt tgcaacggtg ccaccagcgc
     5461 agattctgcc acgccaacgc taatttatga cgcgaaactg gagatccact ccccgcgcgg
     5521 tgttcgtttc gtcccgatta atggctttca caccgggccg ggcaaagtgt ctcttgagca
     5581 tgacgaaatc ctcgtcgcct ttcattttcc gccacagccg aaagaacacg cgggcagcgc
     5641 gcattttaaa tatgccatgc gcgacgcaat ggatatttca acgattggca gcgccgcaca
     5701 ttgccgactg gataacggca atttcagcga attacgcctg gcatttggtg ttgccgcgcc
     5761 aacgccgatt cgctgccaac atgccgaaca gactgcacaa aatgcgccat taaacctgca
     5821 aacgctggaa gctatcagcg aatctgtcct gcaagatgtc gccccgcgtt cttcatggcg
     5881 ggccagtaaa gagtttcgtc tgcatctcat ccagacgatg accaaaaaag tgattagcga
     5941 agccgtcgcc gcggcggggg gaaaattgca atgaatcaca gcgaaacaat taccatcgaa
     6001 tgcaccatta acgggatgcc ttttcagctt cacgccgcac caggcacgcc gctctcggaa
     6061 ttactccgcg aacaaggact gctaagtgtc aaacaagggt gctgcgtggg tgaatgtggt
     6121 gcctgtacgg tgttggtcga cggcacagca atagacagtt gcttatacct tgccgcctgg
     6181 gctgaaggaa aggagatccg cacgctggaa ggtgaagcga aaggcggaaa actttctcat
     6241 gttcagcagg cttatgcgaa atccggcgca gtgcagtgcg ggttttgtac gcctggcctg
     6301 attatggcta ccacggcaat gctggcgaaa ccacgcgaga agccattaac cattacggaa
     6361 attcgtcgcg gactggcggg aaatctttgt cgctgcacgg ggtatcagat gattgtaaat
     6421 acagttctgg attgcgagaa aacgaagtaa aaggatatcc ggcctgaatt caggccggat
     6481 tcactgaggt tatgtgttta acaactcata tttcttaatc ttgcgataga gcgtagcaat
     6541 gccgatgccc agttcatcag caacttgctt cttgctgtta tgacgtgaaa gcgcctcgcg
     6601 gatcatttgc ttttccatct cctccagcgc cgtgccgccc gcatcatcga gtgacaggtg
     6661 cgcctcactg acctctgtta catcactttg ctccgttgtg ccattattca gcagatttgg
     6721 cggcaatagc gtgctgtcga taacttcacc tgaaggaacc acgttaacca gatattccat
     6781 caaattgctt aactcgcgca ggtttccggg ccaacgatgc ttacgcaata tttcgacgac
     6841 atcgggagca atgccaggat aaaccgatcc cagacgacgg gtatgcagat gtaaaaagta
     6901 atgcaccaat agttcaatat cttcctgacg ttcacgcagc ggtggcagag ttatcgggat
     6961 aacattaagt cggtagaaga gatcttcgcg gaatttacct tcggcaatga actgggccaa
     7021 attctgatta gttgcagaaa tgatgcgaat gtcgacttgt attgggctac tggcaccaat
     7081 cggcagaatt tcacgcgcct caatagcgcg cagtaattta gcctgcaaca ttaatggcat
     7141 atcacctatt tcatcgagaa acagcgtgcc cgtattcgcc gcctgaatca accctgtttt
     7201 accgttggca gaagcaccag taaatgcgcc tttaacataa ccgaacagtt cgctttccag
     7261 aagctgctcc ggaatcgcgg cacagttgat agcaataaag ggtttattcc gtcttccgct
     7321 caacttatgg attgctcggg cgacgacttc tttacccgtg ccgctttcac caaccaccat
     7381 aacgctggat gggctgggtg caatacggct aatgagtcgt tttaattgcc gcataacacg
     7441 gcactcgcca accaattgtt caatatgtgg ttcatcaggt gcatttgcta cagaaaaact
     7501 ggtatgcgat tggtgaaatg ccattaaaaa taattgtcgg ccctgaatgt tatgcaattg
     7561 accaatgatt aattcacttt tatcgtccca cgaaacaata tgctgcatat gtccatgagt
     7621 aaaattactc tcaaatgtta atggtctgaa acggataggt ttcccaataa tattattttg
     7681 tacaacacca agtgttttta aggcagtctg attaacaaac tgaacccgac tttcatcatc
     7741 aacaactaat acgccctgat ccatattatc gatcatggtc gcgaatattt tactgatgtt
     7801 atctcctggc ccctgatcct ccagaagttt cgaaacaaaa atggtggata tatggcgaac
     7861 ataatcagaa aattcgcgta aattatcact gatatgctct tgttgctcgt gggtaacggc
     7921 aatcaaactt atcaccccaa cacaacgatc ctgtaaaatg acaggcgtac ccagaaatgc
     7981 tttttcgcgg caattttctt tactatcgca accttcgcaa aggggatcga agcgagactg
     8041 tgtcacaact ttttcagttt tcgtttccag gacgtggcgg agcaggcgtg agttgccgct
     8101 caactggcga ccaagaaact tcccatacgc gcccgttccg gcgacgcgac acaagttttc
     8161 atcaacgatc tcaacctcaa actgcaaaac gctggcaagc attctggcaa aacgctgaat
     8221 tgtcggttga atttgcatca atactgactg cgtagtagca agctccatag ctttaccttc
     8281 cagacttact taaaagtcga tcattgaaga cgttgatggt tcacagatca tgatgatatt
     8341 aactcaggcg aaattggctt tgataaaaac ataagatttt tatcattttc taatgcaatt
     8401 atggaagaga tatcacattt ctatatcaat atgagaatta cggcggtgag tttatcaaac
     8461 tgaagagaga tagcctgccc ctttatctta tttctgatac ttagcagaaa ataaataacg
     8521 cgataaaaaa agccaaacgt tttcgtattt tacaaacaac cagaagctgg catcaatttg
     8581 tgaacaaccc cacacattat ccgtcaaatt agtcttttgc agccgcgcgg ataattctgg
     8641 cacacttatt gttagtccca ggtatagcta tgaaaacacc aatcactttg gcaagtcaca
     8701 gtgaaataaa ccactttgcc tgtcattcca ctaccgggac tgtatgatga aaactgttaa
     8761 tgagctgatt aaggatatca attcgctgac ctctcacctt cacgagaaag attttttgtt
     8821 aacgtgggaa cagacgccag atgaactgaa acaagtactg gacgttgccg cagcattaaa
     8881 agcactgcgt gctgaaaaca tctcaaccaa agtctttaat agtggattag gtatttccgt
     8941 attccgcgac aactccaccc gtacccgctt ctcttatgct tccgcgctta acctgctcgg
     9001 ccttgcacaa caagatctcg acgaaggcaa atcacaaatc gctcacggcg aaaccgtgcg
     9061 tgaaaccgcc aatatgatct ccttctgcgc cgacgctatt ggtattcgcg acgatatgta
     9121 tctgggcgca ggcaacgcct atatgcgtga agttggcgct gcacttgatg acggttacaa
     9181 gcagggtgta ctgccacagc gtccggcttt agtgaacctg caatgcgata ttgaccaccc
     9241 gactcagtca atggctgacc tcgcgtggtt acgtgaacac tttggttcac tggaaaacct
     9301 gaaaggtaaa aaaatcgcca tgacctgggc ctactctcca agctatggca aaccgctctc
     9361 tgtaccacaa ggcatcatcg gtctgatgac tcgcttcggt atggatgtca ccttggccca
     9421 tccggaaggc tacgacctga tcccggatgt ggttgaagtg gcgaaaaaca atgctaaagc
     9481 ctccggtggt agcttccgtc aggtcaccag catggaagaa gccttcaaag acgcagacat
     9541 cgtttatccg aagtcatggg caccttacaa agtgatggaa gagcgtactg aattgctgcg
     9601 tgcgaacgac cacgaaggct taaaagcact ggaaaaacag tgtctggcac agaacgcgca
     9661 acacaaagac tggcattgta ctgaagagat gatggaactg acccgtgatg gcgaagccct
     9721 gtacatgcac tgcctgccag ctgatatcag cggcgtatcc tgcaaagaag gtgaagtgac
     9781 tgaaggcgta ttcgaaaaat accgtatcgc tacctacaaa gaagccagct ggaagcctta
     9841 tatcatcgcc gcgatgatcc tgtcccgtaa atacgccaaa ccaggtgcac tgctcgagca
     9901 actgctgaaa gaagcgcaag aacgcgtgaa ataacccctc gggccgatct gctggtcggc
     9961 cctctatttc cagaggccaa aaggatagga tatgtccgtt ttctcattga agattgatat
    10021 cgccgataac aaatttttca acggcgaaac atcaccgctc ttttcgcaaa gctaggccaa
    10081 actggcgcgc cagttccacc agaaaatagc tggttatcgc ccaacaccgc tttgtgcgct
    10141 ggacgatctc gcaaaccttt ttggtgtgaa gaaaattctc gtcaaagacg aatcaaaacg
    10201 attcggtctg aacgccttca aaatgcttgg cggtgcgtac gccatcgctc aattattgtg
    10261 tgaaaaatat catcttgata tcgaaacgct gtcatttgag cacctgaaaa atgccatcgg
    10321 cgaaaaaatg actttcgcga ccaccaccga cggcaaccac gggcgcggtg tggcgtgggc
    10381 agcacagcaa ctcggacaga atgcggtgat ttacatgccg aaaggttctg ctcaggaacg
    10441 cgttgacgcc attctgaacc tcggtgccga gtgcatcgtc acggatatga actatgacga
    10501 taccgttcgc ctgaccatgc aacacgcgca gcagcacggc tgggaagtgg tacaggacac
    10561 ggcatgggaa ggttacacca aaatcccaac ctggatcatg caaggctacg caaccctggc
    10621 agatgaagcc gtcgagcaaa tgcgtgaaat gggcgtaacc ccgacgcacg ttctgctgca
    10681 agccggtgtc ggagcaatgg ccggtggtgt gctgggttat ctggtcgacg tctacagccc
    10741 gcaaaatctg cacagcatta ttgttgaacc tgacaaagct gactgtattt atcgctccgg
    10801 cgtcaaaggc gacatcgtca acgttggcgg tgatatggcc accatcatgg cgggcctggc
    10861 ctgtggcgaa cctaacccgc tgggctggga aatcctacgt aactgcgcca cccaattcat
    10921 ctcctgccag gacagcgttg ccgcattagg tatgcgcgtg ctgggtaatc cgtacggcaa
    10981 cgacccgcgc atcatctccg gtgaatccgg cgctgtcggt ttgggcgttc tcgcagcggt
    11041 tcattatcac ccgcaacatc aaagcctgat ggaaaaactg gcgctggaca aagatgccgt
    11101 agtgctggtt atcagcactg aaggcgacac cgacgtgaag cactaccgcg aagttgtctg
    11161 ggaaggcaaa cacgctgtag caccttaatc accctattgg aactactccc tgcaaaacgg
    11221 ggagtaaaaa atctggagaa aaataatggc taagaatatt ccattcaaac tgattcttga
    11281 aaaagcaaaa gattaccagg cggacatgac tcgcttcctg cgcgacatgg ttgctattcc
    11341 cagtgaaagc tgcgacgaaa aacgcgtagt acatcgtatt aaagaagaga tggaaaaagt
    11401 cggcttcgat aaagttgaaa tcgacccgat gggcaacgtt ctcggttata tcggccacgg
    11461 cccgcgtctg gtggcaatgg acgctcatat cgataccgtc ggcattggca acatcaaaaa
    11521 ctgggacttc gatccgtacg aaggcatgga aactgatgag ctaatcggtg gtcgcggtac
    11581 ttccgaccag gaaggcggca tggcatctat ggtttatgcc ggtaaaatca ttaaagacct
    11641 cggtctggaa gatgaatata ccctgctggt taccggtact gtgcaggaag aagactgcga
    11701 cggtctgtgc tggcagtaca ttattgaaca atccggcatt cgcccggaat ttgtggtcag
    11761 taccgaacca accgactgcc agatataccg tggtcagcgc ggtcgtatgg aaattcgtat
    11821 tgatgttcag ggtgttagct gccacggttc tgcgccagaa cgcggtgaca acgccatttt
    11881 caaaatgggt ccgattcttg gcgaattaca agaactctcc caacgtctgg gttatgacga
    11941 attcctcggc aaaggcaccc tcaccgtttc tgaaatcttc ttcacatccc caagccgttg
    12001 cgctgtagca gatagctgcg ccgtctctat tgaccgccgt ctgacctggg gcgaaacctg
    12061 ggaaggcgcg ctggacgaaa tccgcgccct gcctgcagta cagaaagcta acgcggttgt
    12121 ttctatgtac aactacgacc gtccgtcctg gactggcctg gtttacccaa ccgaatgcta
    12181 cttcccgacc tggaaagtgg aagaagatca cttcaccgtt aaagcactgg tgaatgccta
    12241 cgaagggctg tttggcaaag cgccggttgt tgataagtgg accttctcaa ctaacggcgt
    12301 atctatcatg ggccgtcacg gcattccggt gatcggcttt ggcccgggta aagaacctga
    12361 agcgcatgca cctaacgaaa aaacctggaa atctcacctg gtgacctgtg ccgcgatgta
    12421 cgctgcaatc ccgttaagct ggctggcaac agagtaatta ctcttcactt attcccctcc
    12481 ggttcgccgg agggtttttg gagtttgcta tgcgcgtatt gatcaaaaac ggcactgtcg
    12541 ttaacgcaga tggacaagcc aaacaggatt tgctgattga aagcgggatt gttcgccagt
    12601 tgggcaacaa tatttcgccg cagctcccgt atgaagaaat tgatgccact ggctgttacg
    12661 ttttccctgg cggcgtggat gtccatacgc atttcaatat tgatgtcggc atcgcgcgca
    12721 gttgtgatga ttttttaccg gtacccgcgc agctgcgtgt ggcggtacaa caaccattat
    12781 tgaccatatg ggatttggcc caaacggctg tcggttacgc catcaactgg aggtttatcg
    12841 tggttatgcc gcccataaag cggtcatcga ttacagcttt cacggtgtga tccagcacat
    12901 taatcacgca atcctcgacg aaatcccgat gatggtcgag gaaggactga gcagttttaa
    12961 actctattta acctatcaat acaaactcaa cgatgacgag gttttgcagg cattacgccg
    13021 cttgcatgaa tccggcgcgc tgaccaccgt gcacccggaa aatgatgcgg ctatcgccag
    13081 caagcgggcg gaatttatcg ccgcagggtt aaccgcgccg cgctatcatg ccttgagtcg
    13141 ccctctggaa tgcgaagcgg aagccatcgc ccgcatgatt aacctggcac aaattgccgg
    13201 taacgccccg ctctatatcg tgcacctgtc taacggctta ggtctggatt atctgcgtct
    13261 tgcccgtgcg aatcaccagc cagtctgggt tgaaacctgc ccacaatatc tcctgttgga
    13321 cgaacgcagt tacgatacag aagatggcat gaagttcatt cttagcccac cgctgcgtaa
    13381 cgtacgcgag caggacaaac tgtggtgtgg catcagcgat ggtgcgattg acgtggtggc
    13441 gaccgatcac tgcaccttct cgatggctca acgcctgcaa atttctaaag gcgatttcag
    13501 tcgctgccca aatggcttac ccggtgtgga aaaccgcatg cagttactgt tttccagtgg
    13561 cgtgatgacg ggacgtataa caccggaacg ctttgttgaa ttaaccagcg caatgcccgc
    13621 caggctgttt ggcctgtggc cgcaaaaagg attattagcg cccggttccg atggcgacgt
    13681 ggtgattatc gacccacgtc agagccaaca aattcagcat cgccatctcc acgacaacgc
    13741 cgactactcg ccatgggagg gttttacctg tcagggcgcg attgtcagaa ccttatcccg
    13801 tggtgaaacg attttctgtg acggcacctt tacaggcaaa gccgggcgag gtcgtttcct
    13861 gcgacgcaaa ccgtttgtcc ctcccgtgct ctaaccactg tcagggatct atacacaatc
    13921 aagaatgatg agtgaggaaa aatgagtaag aaaattgttc tcgccctggg cgggaatgcg
    13981 ctgggcgatg acctggccgg acaaatgaaa gcggtaaaaa ttacttctca ggcaattgtt
    14041 gatttaattg cccaaggaca tgaagttatc gtcactcatg gtaacgggcc gcaggtgggc
    14101 atgattaacc aagcctttga agctgccgca aaaactgaag cacactcgcc aatgctgccg
    14161 atgtctgtct gtgttgcctt aagccagggt tatattggtt acgatctaca aaacgcgtta
    14221 agagaagaac tgctttctcg gggcattaat aaacctgtag caacgctggt tactcaggtc
    14281 gaagtcgacg ctaacgatcc ggcattcctc aacccgacca agccgatcgg ttcgttcttt
    14341 accgagcaag aagcagagca actgacaaaa cagggttaca ctctgaaaga agatgctggt
    14401 cgcggctatc gccgtgttgt tgcctcgcca aaaccggttg atatcattga aaaagaaacg
    14461 gttaaagctc tggtagatgc aggccaggtg gtgatcgccg ttggcggtgg tggtatccct
    14521 gttattcgtg aaggtaacca tctgcgtggt gccagcgcgg ttatcgataa agactgggcc
    14581 agcgcccgtt tagcagaaat gattgatgcc gatatgctga tcattctgac tgcggtagaa
    14641 aaagtggcca ttaacttcgg taaagagaat gaacagtggc tcgatcgcct gtcattaagt
    14701 gatgctgaac gctttatcga agaaggtcat tttgcgaaag gttctatgct gcctaaagtg
    14761 gaagctgccg cctcgtttgc tcgctctcgc gcaggtcgtg aagcactgat taccgtattg
    14821 agcaaagcga aagaagggat tgaaggcaaa accggaacgg tgatttgtca gtaattttaa
    14881 ggccgggtgg gtaataccat ccggccattt tcatttaagc cacttccagc acttcttttg
    14941 tcgctttcac gccctgatgc atcaacatca ttaacgcctc aagtacgccg ccgccaatcg
    15001 cccgggcttt atcagaaacg ctggtgaaat cagcggtttc accacgagga tcgatatcac
    15061 caattttaaa accaccaacc actgccaggc cgtcgttcaa caagccacgc accatccccg
    15121 tcaacggtgc tttaatttca tgctcaccaa tccaggcaat cacatcgccc tctttcacca
    15181 gatcgcctaa tttcacgttg gatcgcataa tgcctgcagc aggagcacgg atcacccgtc
    15241 gggtggtatg ccccataata ttgccaggaa catcggtatt ctcctgcgca caaccagagt
    15301 aaatcacctg accgagccag tgcccgcgat ttgtttcaat taccgcatga caatccttcc
    15361 ctgcagtaaa gcccggccca agcgcgattg ttactggtgc catatctgcc cgcgttccca
    15421 aattctgttt cgccagaata gcgtccacca cgcaaagcgg tttcagttca tcaagcaatg
    15481 aacaggcggg atctaccatc acagggatga atccgcgttc ggtaagtttc atcgcttccg
    15541 cagagctggt tgccaggcga gcagtgacgc cttcgaccgt catttcgcca tcgaacacgg
    15601 cctgggcaaa cgccacggta caacgaatca ctgtcggttt ttccacttcc agcatgatca
    15661 ctttaaaacc cgcatgatac agacgtagcg ccacaccact ggcgatatct cccgcaccgc
    15721 gaatcaccac cagctggtgt ccggaaggat gagaaggttt catcatcagc ccacccggcg
    15781 cgttattttt cacccataat atttctgcca gcacgctgat ggcgatctcc tgcggcgttt
    15841 ccgcacctat gttgtaacca acgggcgcat gtaaacgggc aatatgttct tcagccacgc
    15901 ctttctcacg caattgacgc aggaaaagct gaaccttgcg gcgacttgcc agcaacccca
    15961 accatgcaat gggctgttca atgagtttgt cgagggcttc acgatcctga ttattcgtgg
    16021 caatcaggac aaaattatca gggcgaatat ccagttcttc cactgccgca ccaaacgact
    16081 cagcatggag aagcgtggtt gatggtggga atagttcagg attgaggctt tcgcgataaa
    16141 tatcggcaac ggcaatatca aatcctaata gcgccgcact ctgggctatc gcccggttga
    16201 catgccccgc gccaatcaac accagacgtg gacgcatacc atggacgctg ataaatactg
    16261 acattgcacc tccacaatct gacccgacag catccgcacc gttacgagcc atacgtccat
    16321 ggaataatcg cggcttacgt tcctgcaatg cctgaagtga ctcttcaatc accttccgct
    16381 caaccattcc gccaccaatt gtaccgacga tagaaccatc ggcgcgcact aacatttgtg
    16441 cagaatggcg gggagtcgag cctcggctat caacaatttg cgccatcgca aacggacaat
    16501 tttgctcttc gagttttgca gcctctgtga aaatattcat accaacctca atggatatcc
    16561 tttcagtaac ccggaatacc cgggccgcta attcacaaat ctgcgcgcga ttgcaggatg
    16621 ttcttgtata tcgcccagcc agattgcctc tacgtcgtgt tgttgcagcg gttgaaggag
    16681 ctcgctttgt gcaatcgcat tctcacattg agaaaaacgg ttaatgaacc agactcgccg
    16741 acaaccttgc ggtacgtttt taaacgcccc ctgagggtgg cgaaccagcg caacgagatc
    16801 gctcagttgc aaggttgcat caggtgttaa cccggtaata tcagcaaact gcgaccagcg
    16861 atggacattt tctgtgctca ctttcgcgcc caaagtatgt cctcccatca cagcaatcac
    16921 gcagcaactg cttttgggta tgcaaggttc gtgctcatca ggcgctttta acggcattcc
    16981 acgcgagcca tctgcttcaa tgagaattac gtcacactct ggtcgttgca ccagtgcatc
    17041 aatcgcttct ggcgtaaatc cctgcacttt tccctggttc gctttccagc tgtgaaaaca
    17101 aaatgaaatg ggggatgtaa gagacgcatg aggaagcatg gcgggatcac gacagaaaac
    17161 cacgggccag tgagacgttg gcataaacat atgtgtcgtc gtcgtgatta acacacgcct
    17221 gccgctagcc tggagcagtt ctgccagcca aaaaagcaag ctggttttcc ccccagcacc
    17281 aacaacagaa atcacagtgg gacgtttctg cgcacctaaa tcaatgacta atgccgatgg
    17341 gtcaactata cttttcacga gtctttatga cctctctggg ataaattatc cccaacttaa
    17401 tccatcagga agtaacgcaa ttatcaggcg ttattagccc ctataaataa tggaaccact
    17461 atgtcagcca tcgactgtat aattaccgct gccggattat catcaagaat ggggcaatgg
    17521 aaaatgatgt tcccctggga acagggaaca attcttgata caagtatcaa aaatgcgttg
    17581 cagttttgta gccgaattat tttagtcacc ggctatcgtg gtaatgaact gcacgaacgt
    17641 tatgcgaacc agagcaatat cactattatt cacaacccag attatgcgca gggtttacta
    17701 acatccgtca aggccgcagt acccgcagtg caaacagaac attgttttct cacccacggt
    17761 gacatgccaa ccctcaccat cgatattttt agaaaaatct ggtcgttacg aaatgatggc
    17821 gcaatactgc cactccataa tggcatcccc ggccatccga ttttagtgtc aaaaccatgc
    17881 ctgatgcagg caatccagcg acccaatgtc accaatatgc gtcaggcact ccttatggga
    17941 gatcattatt ccgtcgaaat agaaaatgca gaaataattt tagatattga tactccggac
    18001 gactttatta cagcgcaaaa aaggtatact aaaatttaga aaatgtagtt aaacgattgc
    18061 gttcaaatat ttaatctctc cagcactctt tatcataatg ataaggaatc actgattttg
    18121 agaaaagaga aaaagtgata aatgggctga caataataat tttcaggagt gattttaatt
    18181 ctcatttggc acctgtgagt tcattcacaa aaagccttta agtttcgagc cacctcacat
    18241 ttttttatat ttcccgccaa acctggcaag agtggtgcga ttgttgctct atccccctaa
    18301 accaccggat ttctcaacac cggtcactca atgatatctg tataagctaa ggagagggtt
    18361 atgggggata ttatgcgtcc cattccgttt gaggaacttt tgacgcgcat atttgatgaa
    18421 taccaacaac aacgctcaat ctttggtatt cccgagcaac agttttactc acctgtaaaa
    18481 ggtaaaactg ttagcgtctt cggtgaaacc tgtgccactc ccgtcggccc tgccgctggc
    18541 ccgcacacgc agctcgcgca aaatattgtc acttcctggc tgactggcgg acgcttcatc
    18601 gaactaaaaa ccgtccaaat tcttgaccgc ctggagctgg aaaagccctg tattgatgcc
    18661 gaagacgaat gctttaacac cgaatggtct accgaattta ccttgcttaa agcctgggat
    18721 gaatacctca aagcctggtt tgccctgcac cttctcgaag cgatgttaca gccttctgat
    18781 tccggtaaat cgttcatctt taatatgagc atcggttaca acctcgaagg tattaagcaa
    18841 ccgccgatgc aacagttcat cgacaatatg atggacgcat ctgaccatcc gaaattcgct
    18901 caatatcgcg atacgctgaa taaattactc caggatgacg catttttagc ccgccacgga
    18961 ttgcaggaaa aacgcgaaaa cttgcaagcc ttacccgctc gcatccccac cagtatggtg
    19021 cagggcgtcc ccctctccac catgcacggc tgtcctccgc atgaaatcga agccatttgc
    19081 cgctacatgc tggaagaaaa agggctcaac acctttgtca aacttaaccc gaccttactg
    19141 gggtacgcgc gtgttcgtga gatcctcgat gtctgcggtt tcggttacat aggcttaaaa
    19201 gaagagtcat ttgatcacga cctcaagctg acgcaagcac tggaaatgct ggagcgcctg
    19261 atggtactgg caaaagaaaa atcactcggc tttggcgtaa aactgactaa cactctcggc
    19321 accatcaaca ataaaggcgc actgcctggt gaagagatgt atatgtcagg ccgtgcgctg
    19381 ttcccgctct ccatcaatgt tgcagcagtt ctctctcgcg cctttgacgg caaactgccc
    19441 atttcttatt ccggtggtgc cagtcagctg actatccgcg atatttttga tacaggtatt
    19501 cgccctatta ctatggcaac cgacctgctg aaacctggcg gctatctgcg cttaagtgcc
    19561 tgcatgcgcg agctggaagg ctccgacgcc tggggacttg accatgttga cgtcgaacga
    19621 ctgaacagac tggcagcaga cgcgttaacc atggaataca cccagaaaca ctggaagcca
    19681 gaagagcgta ttgaagtggc agaagacctg ccgctgaccg attgctacgt tgccccctgt
    19741 gttactgcct gcgctatcaa gcaagatatt ccggaataca tccgtctgct tggcgaacac
    19801 cgctatgccg acgcgctgga actcatctac caacgcaacg ccctacctgc cattaccggt
    19861 catatttgcg atcaccagtg ccaatacaac tgtacccgcc tggattacga cagtgcgctg
    19921 aatatccgcg aactgaaaaa agtcgcgctg gaaaaaggtt gggatgaata taagcaacgc
    19981 tggcacaaac ccgccggttc tggttcacgc catccggttg ccgtgattgg tgcaggtccg
    20041 gcgggtctgg cagcaggtta cttccttgcc agagcgggcc atccggttac gctgtttgaa
    20101 cgcgaagcca atgctggcgg cgtggtgaaa aatatcattc ctcagtt
//
LOCUS       DAEEKQ010000067        19837 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.067, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000067 DAEEKQ010000000
VERSION     DAEEKQ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19837
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.067"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            296..1066
                     /locus_tag="KLK57_002281"
     CDS             296..1066
                     /locus_tag="KLK57_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HBE0892640.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1057..1530
                     /locus_tag="KLK57_002282"
     CDS             1057..1530
                     /locus_tag="KLK57_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HBE0892641.1"
                     /translation="MGVIEFLFALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1638..2177
                     /gene="dnaT"
                     /locus_tag="KLK57_002283"
     CDS             1638..2177
                     /gene="dnaT"
                     /locus_tag="KLK57_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HBE0892642.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2180..2917
                     /gene="dnaC"
                     /locus_tag="KLK57_002284"
     CDS             2180..2917
                     /gene="dnaC"
                     /locus_tag="KLK57_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HBE0892643.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2966..3460
                     /locus_tag="KLK57_002285"
     CDS             2966..3460
                     /locus_tag="KLK57_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HBE0892644.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3714..6005
                     /gene="opgB"
                     /locus_tag="KLK57_002286"
     CDS             3714..6005
                     /gene="opgB"
                     /locus_tag="KLK57_002286"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HBE0892645.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWQGKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6144..7166)
                     /gene="lgoD"
                     /locus_tag="KLK57_002287"
     CDS             complement(6144..7166)
                     /gene="lgoD"
                     /locus_tag="KLK57_002287"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HBE0892646.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7305..8219
                     /locus_tag="KLK57_002288"
     CDS             7305..8219
                     /locus_tag="KLK57_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE0892647.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVVVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8434..9785
                     /locus_tag="KLK57_002289"
                     /pseudo
     CDS             8434..9785
                     /locus_tag="KLK57_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(9834..11489)
                     /gene="tsr"
                     /locus_tag="KLK57_002290"
                     /pseudo
     CDS             complement(9834..11489)
                     /gene="tsr"
                     /locus_tag="KLK57_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
     gene            complement(11608..12054)
                     /gene="hpaR"
                     /locus_tag="KLK57_002291"
     CDS             complement(11608..12054)
                     /gene="hpaR"
                     /locus_tag="KLK57_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HBE0892648.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
     gene            12326..13615
                     /locus_tag="KLK57_002292"
     CDS             12326..13615
                     /locus_tag="KLK57_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HBE0892649.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTHKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEDTAK"
     gene            13612..15078
                     /gene="hpaE"
                     /locus_tag="KLK57_002293"
     CDS             13612..15078
                     /gene="hpaE"
                     /locus_tag="KLK57_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HBE0892650.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADSLGELALEADIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15080..15931
                     /gene="hpaD"
                     /locus_tag="KLK57_002294"
     CDS             15080..15931
                     /gene="hpaD"
                     /locus_tag="KLK57_002294"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HBE0892651.1"
                     /translation="MGKLALAAKITHVPSMYHSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            15941..16321
                     /locus_tag="KLK57_002295"
     CDS             15941..16321
                     /locus_tag="KLK57_002295"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HBE0892652.1"
                     /translation="MPHFIVKCSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            16432..17235
                     /gene="hpaH"
                     /locus_tag="KLK57_002296"
     CDS             16432..17235
                     /gene="hpaH"
                     /locus_tag="KLK57_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HBE0892653.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17246..18034
                     /gene="hpaI"
                     /locus_tag="KLK57_002297"
     CDS             17246..18034
                     /gene="hpaI"
                     /locus_tag="KLK57_002297"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HBE0892654.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HTPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATAVKPGVY"
     gene            18209..19585
                     /gene="hpaX"
                     /locus_tag="KLK57_002298"
     CDS             18209..19585
                     /gene="hpaX"
                     /locus_tag="KLK57_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HBE0892655.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLSVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGMLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATL"
     gene            19595..>19837
                     /locus_tag="KLK57_002299"
     CDS             19595..>19837
                     /locus_tag="KLK57_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HBE0892656.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPS"
BASE COUNT         4732 a         5347 c         5119 g         4639 t
ORIGIN      
        1 atttattcaa tgaaatctga tggttttaac ttgttaacat taaaaaacgt gcccggttat
       61 ctattatcct gattcgtaaa ggccaaacaa taaattcatt atagtgataa tattcatgtg
      121 atagtttgtt tgcggcgaga gataattcgc tttttatcac cgagcttgtc ccgataacca
      181 ggattaatca gcgcaatttt gatcttaacc taccgcaaaa caaccgtgct tcatgataca
      241 aatggattct cattatctga acaatacgca gcacgtctat gacaaagggc gagttatgca
      301 aactgagcaa cagcgagccg taacacggct ttgtatccag tgtggattat ttcttttgca
      361 acatggtgcg gaaagcgcgt tggttgatga gctttcctca cgactgggtc gggcgttggg
      421 gatggacagc gtcgaaagtt ctatctcttc gaacgccata gtgctgacaa ccattaaaga
      481 tgggcaatgc ctgacatcga cacgtaaaaa tcacgatcgc ggcattaata tgcatgtggt
      541 gactgaagtc cagcacattg tgattctggc ggagcaccat ctgctggatt acaaaggcgt
      601 agagaaacga tttagccaaa ttcagccatt acgttaccca agatggctgg tagccttaat
      661 ggttggcctt tcttgcgcct gtttctgcaa actcaataaa ggtggctggg atggtgccgt
      721 catcaccttc tttgccagta cggccgcgat gtatatccgc cagctgctgg cacaacgtca
      781 tcttcatccg cagatcaact tttgcctcac cgctttcgcc gccaccacca tttccggatt
      841 gcttttgcaa cttcccactt tcagcaatac acccactatc gcgatggccg ccagcgttct
      901 gctgctggtg ccgggatttc cgttgattaa tgccgtcgcc gatatgttta aaggccacat
      961 caataccgga ctggcacgct gggcgatcgc cagtctgctg acactggcta cctgcgtcgg
     1021 cgtggtgatg gcactgacga tttgggggct acgcggatgg gtgtgatcga gtttctgttt
     1081 gcattggcgc aggatatgat cctcgccgcc attccagcgg tcggctttgc gatggtgttc
     1141 aacgttcccg ttcgggcatt acgctggtgt gcgctgcttg gcgcgattgg tcatggttca
     1201 cgaatgatct taatgaccag cgggctgaat attgagtggt caacctttat ggcctccatg
     1261 ctggtcggca ccattggtat tcaatggtcg cgctggtatc tggcgcatcc gaaagtgttt
     1321 accgtggcgg ccgttatccc tatgttcccg ggcatatcgg cttataccgc aatgatttcg
     1381 gcggtaaaaa tcagccagtt aggttacagc gaaccgttga tgattaccct gttaaccaac
     1441 tttcttacag cttcatcgat tgttggtgcg ttatccatcg gtctttccat tcctggatta
     1501 tggttgtacc gcaagcgccc tcgcgtataa aattgcctcg cttaaagcac acggatgaga
     1561 gacagcctcc tctcctccgt gtgttactat aaaagttatc tcccttctcg ttcatcgttc
     1621 catatttgag aaacagtatg tcttccagag ttttgacccc ggacgtcgtt ggtattgacg
     1681 ccctggtaca cgatcaccaa accgttctgg caaaagctga aggcggtgtg gttgccgtat
     1741 ttgctaacaa tgccccggcg ttttatgccg tcacgcctgc acgcctggct gaactgctgg
     1801 cgctggaaga aaagctggcg cgtccgggaa gcgatgtcgc tctggacgat caactctatc
     1861 aggaaccgca agccgctccc gttgctgtac ccatggggaa attcgccatg tatccggact
     1921 ggcaacccga tgccgatttt atccgcctgg cggcgctatg gggtgtggca ctaagagagc
     1981 cggtgaccac cgaagaactg gcctcattca ttgcctactg gcaggcggaa ggtaaagtct
     2041 ttcaccatgt gcagtggcaa caaaaactgg cgcgcagcct gcaaatcggt cgtgccagca
     2101 acggcggact gccgaaacga gatgtgaata cggtcagcga acctgacagt caaattccac
     2161 caggattcag agggtaacga tgaaaaacgt tggcgacctg atgcaacgcc tgcaaaaaat
     2221 gatgcctgcc catatcaaac ctgcattcaa aacgggtgaa gaactactgg cctggcagaa
     2281 agaacaagga gcgatccgct ccgccgctct cgaacgtgaa aatcgggcga tgaaaatgca
     2341 gcgcaccttt aaccgctccg gtattcggcc actgcatcag aactgctcct ttgagaacta
     2401 tcgcgttgag tgtgaagggc agatgaatgc gttaagcaaa gcgcgccagt acgtcgaaga
     2461 gtttgacggc aacatcgcca gcttcatctt ttctggtaag ccaggaaccg gcaaaaacca
     2521 tctggcggcg gcaatctgca acgagctgct gctacgcggt aaatccgtat tgatcatcac
     2581 cgtggccgat attatgtcgg cgatgaaaga taccttcagg aatagcggta ccagtgaaga
     2641 acaactgctt aacgatctga gcaacgttga tctgctggtg atcgatgaga tcggcgtgca
     2701 gaccgaatcg aaatacgaaa aagtgatcat caaccagatc gtcgatcgcc gctcttcttc
     2761 caaacgccca accgggatgc tgaccaacag taatatggaa gagatgacca agttgctggg
     2821 cgaacgcgtg atggaccgta tgcgcctggg taacagtttg tgggtgatct tcaactggga
     2881 tagctaccgt agccgggtaa caggtaaaga gtattaatcc ccgtttctgg cggtatactg
     2941 cgacacaatt ccaccttgag taatgatgat gaaaactgtt aaacatcttc tgtgctgtgc
     3001 cattgccgcc agcgcattaa tttccaccgg ggtgcatgct gcgtcctgga aagatgcgct
     3061 ctccagcgcc gccagcgaac ttggcaacca aaacagcacg acacaggaag gcggttggtc
     3121 gctcgcgtca ttaactaact tgcttagcag cgggaaccag gccttaagcg cagataacat
     3181 gaacaacgcc gcaggcattc tgcaatactg cgcgaagcaa aagctggcgt cggtaaccga
     3241 tgccgaaaac atcaagaacc aggtgctgga aaagctgggc ctgaacagtg aagagcaaaa
     3301 agaagacacc aactatctgg atggcattca gggtttgctg aaaaccaaag atggtcagca
     3361 actcaatctg gataacatcg gaacgactcc gctggcagaa aaggtgaaaa ccaaagcctg
     3421 cgatctggtg ttaaagcagg ggctgaactt catttcctga accgcgaaac gccgcgtttt
     3481 agctgttacc cggctgacgc ggctctctcc tgcccttttc cctgctcctg gacggttttc
     3541 cccctgttac acgggaagcg accgcgatca aaaaagtctg tttataaact aattcctaac
     3601 aacatcgcat ttttttgaca ctagaattgc agcaatatgg cgacgccatt acattgtgct
     3661 tctcaaaaag cttaaacgtt ggctaaacgg cccggtttag tgaggatcgc ccgttgtcag
     3721 aactactctc tttcgccctt tttctcgcct ctgtgctgat ttacgcatgg aaagcgggac
     3781 gtaacacctg gtggtttgca gccacattaa cggtgctggg gctatttgtc gttttaaata
     3841 tcaccctgtt tgccagcgac tattttactg gcgatggtat taacgacgcg gttctctata
     3901 ccttaaccaa cagcctgacc ggtgctggcg tcagcaaata catcctgccg ggtatcggca
     3961 ttgtgctggg gctgacagcg gtgttcggtg cgctgggctg gatcctgcgc cgtcgtcgcc
     4021 atcatccgca ccattttggt tacagcctgc tggcgctctt actggcgctg ggttcggtgg
     4081 acgccagccc ggcatttcgt cagataacgg aactggtgaa atcccagtca cgcgatggcg
     4141 acccggactt tgcggcttat tataaagagc cgtcgaaaac tatccctgac ccgaaactca
     4201 acctggttta tatctacggc gaaagtctcg agcggaccta ttttgataac gaagctttcc
     4261 cggatctcac gcctgaactg ggcgcgttga aaaatgaagg cctggatttc agccacacgc
     4321 agcagctgcc aggaacggat tacacgattg cgggcatggt ggcttctcag tgcggcatac
     4381 cgctgtttgc cccctttgaa ggcaacgcct ccgcctctgt ctccagcttc ttcccgcaga
     4441 acatctgtct gggcgatatc ctgaaaaact cgggttatca gaactatttc gtgcagggcg
     4501 cgaatctgcg ttttgccggt aaagatgtgt tcctgaagtc gcatggcttc gaccacttat
     4561 acggctcaga agagctgaaa agcgtggtgg ccgacccgca ctatcgcaac gactggggat
     4621 tctacgacga taccgttctc gatgaagcgt ggaaaaagtt tgaagagctt tcccgctcag
     4681 gtcagcgatt ctcactgttt accctgacag tcgataccca tcacccggat ggttttatct
     4741 cgcgtacctg taaccgcaaa aaatatgatt ttgacggtaa gccgaaccag tcattcagcg
     4801 cggtaagttg cagccaggag aatatcgcgg cgtttatcaa caaaatcaaa gcgtcaccgt
     4861 ggtttaaaga taccgtcatc gtcgtctctt ctgaccattt agcgatgaac aacacggcgt
     4921 ggaaatacct caataagcag gatcgcaata acctgttttt tgtcattcgt ggcgacaagc
     4981 cgcagcaaga gacgctggca gtgaagcgta acacgatgga taacggcgcg acggtgctgg
     5041 acattctcgg tggcgataac tatctcggac ttggtcgtag cagtttatcc gggcagtcga
     5101 tgtcggaaat cttcctcaat atcaaagaga aaacattggc gtggaagccg gatatcatcc
     5161 gcctgtggaa attccctaaa gagatgaaag agttcaccat cgaccagcag aaaaacatga
     5221 ttgccttctc gggtagccat ttccgtttgc cgctgctgtt gcgggtttca gacaaacgcg
     5281 tggaaccgct gccggaaagc gaatactcag caccgctgcg tttccagctg gccgatttcg
     5341 ctccacgcga caatttcgtc tgggttgacc gttgctataa gatggcacaa ctctgggctc
     5401 cggaactggc actctccacc gactggtgtg tctcgcaagg gcagcttggc ggtcagcaaa
     5461 ttgttcagca tgttgacaaa acaacatggc agggcaaaac ggcatttaaa gatacggtca
     5521 tcgacatggc gcgttataaa ggcaatgtcg atacgctgaa aattgttgat aacgatattc
     5581 gctacaaagc cgacagtttc atcttcaacg tcgccggtgc gccagaagag gtgaaacagt
     5641 ttagtgggat ttcccgtcca gagtcgtggg gccgctggtc caacgcgcag ctgggcgatg
     5701 aagtaaaaat cgagtacaag catccgctgc caaagaaatt tgacctggtg attaccgcca
     5761 aagcatacgg caataacgcc agccgtccta ttccggtacg cgtaggcaat gaagaacaaa
     5821 cccttgtgct gggcaatgaa gtgaccacca ccacactgca tttcgataac ccaaccgatg
     5881 ccgacacact ggtaattgtg ccgccggaac ctgtctcaac caacgaaggg aatatcctcg
     5941 gacactcgcc gcgtaagctc gggatcggca tggtggaaat taaagtggtg gaacgtgaag
     6001 gataattcct gactgatgtg taggtcgcat aaggtgcgac ctacacacct tctgcggcct
     6061 acaaaacatc attgagaaac agaaaaaaac agcgcccaac acttccccgg acgctgcatg
     6121 gcaactttaa taatctcgcc ttatcagaaa gtaattacac ccttaatcaa ctcacgattg
     6181 ttaatcacat cgcgctcgta ggtttctgcc agcgtggcga acggatagcg atgggttaac
     6241 atcatgtcag cagtgatttt tccttccgcc atcagtcgac cgactttagc aaaatcttcc
     6301 ggcgtggcgt tgcggctacc catcatcgtc gtttcttttt tatggaattc cggatcggag
     6361 aactgcaact cacctttaaa caggccgaca aataccaccg tgccgccgtg acgaatcaaa
     6421 ttcacggtgt tattcatcgc atgttgatta cctgtcgcgt cgatcacttt ctgcgccagc
     6481 gaaccaccaa actgcgcccg tagctgcgcg tcaaaatctt cggctgacgg gtccagtaaa
     6541 ggtaattcca gacgcgttgc cacatgttca cggcgcgccg gactggtatc cgccaccacc
     6601 acctgtgcgc catcggcttt agcgattgcc gccgcgccca gaccgattgg ccccgccccg
     6661 accaccagca cctgctcgcc gggagcaatg gctgcgcgac gcaccgcatg agcgctaata
     6721 gcgaaaggtt caatcaatgc tgccgcctgc gggtcaatac cgtctgcggg caaaatgttc
     6781 gccaccggca cgctcaaata ctcactaaaa ccgccatcct gatgcacgcc aatgactgaa
     6841 attttttcac agcaattggt acgcccgctt ttacacgccg ggcattgctg acaggcaaca
     6901 taagggatca cagcaacttg ctgaccattt ttaagatcag caatattttt acccagccca
     6961 acaatctccc cacatatttc atggcctaaa acacgtggat aactaaaaaa tggttgattt
     7021 ccaccccagg catgaatatc ggtaccgcaa atcccgacag acttaatttt tattaatgct
     7081 tcattgtcac ccggaatagg tatctcgcgt tgtttccaga ctaattcttt cggctgctgg
     7141 caaattaaaa cattcatcgt agacatgatt cggctctcca ttttttgttg ccttagatat
     7201 caatgaaaat gaaaaatggc agcggaacgg aaaatctgtt ttgtgaaaac acacgcataa
     7261 aaatgtttta aatcgggttt taatggactc acaaggagga aaatatgagt cgttcacaaa
     7321 atttacgcca caatgtgatt aaccaggtca ttgatgatat ggcgcggggt catatccctt
     7381 ccccacttcc atcgcaaagt gcgctggcgg aaatgtacaa catcagccgc accacagtgc
     7441 gtcatattct cagccactta cgcgaatgcg gcgtcttgac gcaggttgga aacgactatg
     7501 ttattgcgcg taaacctgac catgatgacg gtttcgcctg tactaccgct tcaatgagtg
     7561 aacaaaacaa agtgtttgag caggcttttt tcacgatgat aaaccagcgc cagttacgcc
     7621 caggggaaac gttttctgaa ctgcaactgg ctcgggccgc gggtgtcagc ccggttgtag
     7681 taagagaata tcttttaaaa ttcggccgtt ataatcttat tcacagcgaa aagcgcggcc
     7741 agtggagcat gaagcaattc gatcaatcct acgccgagca gctgtttgag ctacgcgaaa
     7801 tgctcgaaac acattcactt cagcactttc tcaacctgcc tgatcatgat ccccgctggc
     7861 tgcaggcgaa aaccatgctc gaacgtcatc ggctactgcg tgacaacatc ggcaatagct
     7921 ttcgcatgtt ctcgcaactt gacagagatt ttcactcctt actgctttca gctgccgaca
     7981 atatcttttt tgatcaatcg cttgagatca tctcagtcat ctttcatttt cactatcaat
     8041 gggacgaaag cgatcttaaa cagcgcaata tcattgccgt cgatgagcat atgaccattc
     8101 tcagcgctct gatttgccgc agtgaccttg atgccaccct tgcgctccgt aaccatttga
     8161 attcagctaa acaatcaatg attcgatcca ttaatgagaa cacgcgttat gctcattaag
     8221 tacggattaa aaacagcaaa ctattagcgg gtgcacggtt ttatcaaaag ataaataccg
     8281 ggaaattccc gcttacctat gctcacaatc agtcgactaa aaatgttttt gtgagcggta
     8341 gtaaagtcct aaaactttaa cctgccgtta atgtcaacct tgcgtaattc acaattacgt
     8401 aaacaattaa aattataaat acaggagtcc ggcgtggaaa aagaaaatat caccctcgat
     8461 ccgcgttctt catttactcc atcttcgtcg gcagatattc ccgtgccacc agatggatta
     8521 gttcaacgca gtacccgaat taaacgcatt caaaccaccg ccatgttgtt attatttttt
     8581 gcggcggtaa tcaattatct cgaccgcagt tcgctgtcgg tagcaaattt aacgattcgt
     8641 gaagaattgg gattaagtgc caccgaaatc ggcgctttgc tctccgtgtt ttcactcgct
     8701 tacgggattg cgcaacttcc ttgcggccca ctattggatc gtaaaggccc acgcctgatg
     8761 ctgggactgg ggatgttctt ctggtcactg ttccaggcaa tgtctggcat ggtgcacaac
     8821 tttacgcagt tcgtgttggt gcgtatcggt atggggattg gtgaagcgcc gatgaaccca
     8881 tgcggtgtaa aagtcattaa cgactggttc aacatcaaag agcgcggacg cccgatgggc
     8941 ttcttcaacg cagcttctac cattggcgtt gccgtaagcc caccgattct ggcggcgatg
     9001 atgctggtga tgggctggcg cgggatgttt attaccattg gtgtactggg gatttttctc
     9061 gccatcggct ggtatatgct ctatcgcaac cgcgagcacg tagaactgac tgccgttgaa
     9121 caagcttatc tcaatgcagg tagcgtcaat gcccgccgag atccgctcag ttttgccgaa
     9181 tggcgcagcc tgttccgtaa ccgtacaatg tggggaatga tgctcggatt cagtggcatc
     9241 aactacactg cgtggctgta tctggcctgg cttcctggtt acctgcaaac agcctataac
     9301 ctggatttaa aaagcacagg gttgatggcg gctatccctt tcctgtttgg ggctgccggg
     9361 atgctggtca acggttacgt tactgactgg ctggtcaaag ggggaatggc tccgattaaa
     9421 agccgtaaga tctgcattat tgccgggatg ttctgttctg ccgcctttac gctggtcgta
     9481 ccgcaagcga caacatccat gacagcggtt ctgctgattg gcatggcact gttctgtatt
     9541 caacatcctg ctggggcttg atccacgtcg cagttgcttc tcgcatgact gcgtcggtgg
     9601 gcagtatcca gaactttgcc agcttcatct gcgcctcttt tgcgccgatc attactggtt
     9661 ttattgttga taccacccac tcattccgtc tggcactaat catctgcggt tgcgtcaccg
     9721 cagcgggggc actggcgtac atcttcctgg ttcgtcagcc gatcaacgac ccacgtaaag
     9781 attaatctcc ttatgcccga taacattttg cttatcgggc attttcatgg cgattaaaat
     9841 gtttcccagt tctcctcgct atctgccacg gccattttac gcggcgcagc tggcgtcacg
     9901 gtttttacca cagccgatgt ttcacgctgc tgttgctgaa tccggaacac tgccactgct
     9961 tcggtcaggc gactggcctg ctcttccagc gcggcggcgg cagcggcaga ctcttccacc
    10021 agcgcggcgt tctgttgagt tacccggtcc atctcagcaa ccgctaagcc aacctgatcg
    10081 ataccacggc tctgctcatc agaagcagaa gcaatttcgc ccataatgtc cgtcacgcgg
    10141 gtcacggcgc tgacaatctc cgccattgtt tccccggcgc tttcgaccag cgtagagcca
    10201 acatccactt tccccaccga gtcttcaatc aggcttttaa tttcacgagc cgcctgggcg
    10261 ctgcgctggg ccagattacg cacttctccc gcgaccaccg caaaaccgcg cccttgctca
    10321 cccgcacgcg cagcctcaac cgccgcgttc aaagccagaa tattggtctg gaaggcaatg
    10381 ccgtcaatta cgctgataat atcggcgatt ttctgcgaac tggtggagat atcgcgcata
    10441 gtctgcacca cgttatctac cactttgccg ccgcgttgcg ccgtttcaga agcacttaac
    10501 gccagatggc tggcctggcg cgcattttcg gcgttctgct tcaccgttgc ggtcagttac
    10561 tccatgctgg ctgccgtctc ttccagcgaa gcggcctgtt gctcggtgcg cgaagagaga
    10621 tcgttattgc cggtggcgat ttcgctggca ccgctataga tggcattggc cccgttgcgc
    10681 acatcaccga cggtacgcat cagctctccc tgcatatggc gcaaactctc tgccagttgc
    10741 cccatctcat tagagccatc cacctcaatc ggtttcacca gatcgccgcc tgcaatatga
    10801 cgaatgctgt caatcaggcg attcattggc gctaccagcg aggctttaat accgaaccag
    10861 acggcgaaga tgaccgccag tacgacgatc atcacgccca ccagaatcca catcgcctgg
    10921 ctgtaggagg cattgttatc gctgacggcg atatcataga gccgatcgtt ttgctccatg
    10981 taagccacat actgcttctc gaaaccgtcc tgatatccct gggtcggctg atcaaagaac
    11041 tcgttgattt tgcctgcacc taacagttgg atcagctccg ccagcgcatt gtgataaata
    11101 tcgtaattac gtttgatctc tgccgctgcg gcggtgctct gacgcgggtc acgcggcaac
    11161 gcttcgtaat ccgcccagtt tttttccgcc tgtttcagcg aaatactggc actctgcatc
    11221 agctcagcaa cggttgaacc gctaccaata ttgttctgat ccatcatgta gcggataccc
    11281 gcgcggttga gggtgttacg cgtctgcaac aacgcgaccc agctgccatt cagcgtggat
    11341 tgctgctggc gaatggtttg taaaacagtg aaattttctt tgtcattctt taaggcatta
    11401 aagaacagac cgcctgatgt cagttgtaaa aggccaaaaa cggccaaaac cagcagtaag
    11461 ctggtcacga tcttgatacg ttttaacatg gtttctcttt cctgtggacc gatgcaagat
    11521 tttcggcctg gaaagcaaaa actttatgaa aaggtgtaac ccaccatcca tggaaaaacc
    11581 aggcgctggt tatcagatac taaaaagtta ttcattatcg cccgggatgg cttcttgtcg
    11641 ggaattgccc agagcaataa attcctctaa cagatgggtt aactgttgca ttttctcggc
    11701 agtaaactga gcttcaatct gccgataggc ttcttcaatc tgtgtctgag cgcggttata
    11761 cagcgcctgc ccctctttgg ttagcgagat atagagcttc cgctgatcat tgatcggctt
    11821 taatcgcaac actaaaccgt cgcgttccat ccgcgtgagg atcccggtca ggcttgggcg
    11881 caaaatgcag gcgcgatacg ccagatcgtg aaaatccatt gatgggcttt ccgccagaat
    11941 acgcacgatg cgccactgtt gctcagtcaa attatgccgt ttaacgattg ggcgaaaata
    12001 actcatcgcc gcttctcgcg cctggagcaa cgcaatggtt agtgagtcgt gcattatctt
    12061 tcccctgaaa aattcaaaat cattaatata gaaacagtta atcgtcacgt taccgggact
    12121 gcgctgcccc tgcaattcct gattttatca aaattgatga tattttatta acttactgat
    12181 atttaatatt ttattatctt taaatgtaaa tagtttgtta attagatcac atttacatca
    12241 cttacttttg ccaaactacg caatcgggaa taaaagcaat cattaacata ttaatgatta
    12301 agatgatcga atccgaggag tggtaatgaa aggcactatc ttcgccgtag cgttgaacca
    12361 tcgcagccaa cttgatgcat ggcaggaagc gttccagcaa tccccctaca aagccccgcc
    12421 taaaactgcg gtctggttta ttaaaccgcg caatacggtg attggttgcg gtgaaccgat
    12481 tccctttcca cagggtgaaa aggtactgag cggtgcgact gttgcgctga ttgtgggaaa
    12541 aacggcgacg aaagtacgtg aagaagatgc ggcagagtac atcgccggat atgcgctggc
    12601 taacgacgtc agcctgccgg aagagagctt ttaccgcccg gcaatcaaag caaaatgtcg
    12661 tgatggattc tgccccattg gcgaaaccgt ggctctcagc aatgtcgata atctgaccat
    12721 ctataccgag atcaacgggc gtcctgccga tcactggaat accgccgatt tacaacgtaa
    12781 cgccgcgcag ttgctgagcg ccctgagcga atttgccacg ctgaatccag gcgacgccat
    12841 tctgctcggc acgccacagg cgcgcgtgga aatacagcca ggcgatcgcg ttcgtgttct
    12901 cgcagaaggt ttcccgccgc tggaaaatcc ggtagtggac gaacgtgaag tgaccacgca
    12961 caagagcttc ccaacgcagc cacacccgca cggcacgctg tttgccctcg gcctgaacta
    13021 cgccgaccac gccagcgaac tggaatttaa gccaccggaa gaaccgctgg tgttcctgaa
    13081 agcgccaaat accctcactg gcgataacca gacctccgtg cgtccaaaca atattgaata
    13141 catgcactat gaagcggagc tggtggtagt tattggcaaa caggcgcgta acgtcagcga
    13201 agccgatgcc atggattatg tcgcgggcta caccgtgtgt aacgactacg ccattcgtga
    13261 ctatctggaa aactactacc gccctaacct gcgggtaaaa agccgcgacg gactgacgcc
    13321 gatgctttca accatcgtgc cgaaagaggc gatcccggac ccgcataatc tgacccttcg
    13381 caccttcgtc aacggcgagt tacgccagca aggcaccacc gccgatctga tcttcagcgt
    13441 gcccttcctg atcgcctact taagtgaatt tatgaccctg aatccgggcg acatgatcgc
    13501 caccggcaca cccaaaggct tatctgacgt ggtgcctggc gatgaagtag tggtggaagt
    13561 agaaggcgtg ggccgcctgg tgaaccgaat tgtgagtgag gatacagcga aatgaaaaaa
    13621 gtaaatcatt ggatcaacgg caaaaatgtt gcaggtaacg actacttcca gaccaccaat
    13681 ccggcaacgg gtgaagtgct ggcggatgtg gcctctggcg gtgaagcgga gatcaatcag
    13741 gcggtagcgg cagcgaaaga ggcgttcccg aaatgggcca atctgccgat gaaagagcgt
    13801 gcgcgcctga tgcgccgtct gggcgatctg atcgaccaga acgtgccaga gatcgccgcg
    13861 atggaaaccg cggacaccgg cctgccgatc catcagacca aaaatgtgtt gatcccacgc
    13921 gcttcccaca actttgaatt tttcgcggaa gtctgccagc agatgaacgg caagacctat
    13981 ccggttgacg acaagatgct caactacacg ctggtgcagc cggtgggcgt ttgtgcgctg
    14041 gtatcgccgt ggaacgtacc gtttatgacc gccacatgga aggtcgcgcc gtgtctggcg
    14101 ctgggcaata ccgcggtact gaaaatgtcg gaactctccc cgctgaccgc tgacagcctg
    14161 ggtgagctgg cgctggaagc cgatattccg gcaggcgtgc tgaacgtggt acagggctac
    14221 ggcgcaaccg caggggatgc gctggttcgt catcatgacg tacgtgccgt gtcgttcacc
    14281 ggcggtacgg ccaccgggcg caacatcatg aaaaacgccg ggctgaaaaa atactccatg
    14341 gaactgggcg gtaaatcgcc ggtgctgatt tttgaagatg ccgatattga acgcgcgctg
    14401 gacgccgccc tgttcaccat cttctcgatc aacggcgaac gctgcaccgc cggttcgcgc
    14461 atctttattc agcaaagcat ctacccggaa ttcgttaaac gctttgccga acgcgccaac
    14521 cgtctgcgcg tgggcgatcc gaacgatccg aatacccagg ttggcgcgct tattagccag
    14581 cagcactggg aaaaagtctc cggctatatc cgtctcggca ttgaagaagg cgcaaccctg
    14641 ctggcgggcg gcccggataa accgtccgac ctgcctgcgc acctgaaagg cggcaacttc
    14701 ctgcgcccaa ccgtgctggc agacgttgat aaccgtatgc gagtcgccca ggaagagatt
    14761 ttcgggccgg tcgcctgcct gctgccgttt aaagacgaag ccgaaggctt acgtctggca
    14821 aacgacgtgg agtacggcct cgcgtcgtac atctggacac aggatgtcag caaagtgtta
    14881 cgcctggcgc gtggcattga agctggcatg gtgttcgtca acacccagaa cgtgcgtgac
    14941 ctgcgccagc catttggcgg cgtaaaagcc tccggcaccg ggcgtgaagg cggtgagtac
    15001 agcttcgaag tgttcgcgga aatgaagaac gtctgcattt ccatgggcga ccatccaatt
    15061 ccgaaatggg gagtctgata tgggtaagtt agcgttagcc gccaagatca cgcacgtacc
    15121 gtcgatgtat cactctgaac tgccagggaa aaaccacggt tgccgccagg gcgcgatcga
    15181 cgggcataaa gagatcagca agcgttgccg ggaaatgggc gtcgatacca ttatcgtttt
    15241 cgatacccac tggctggtca acagtgctta tcacatcaac tgtgcagacc attttgaagg
    15301 cgtctacacc agcaacgagc tgccgcattt tatccgcgat atgacctaca actacgaggg
    15361 caacccggag ttggggcagc ttattgccga tgaagcctta aagctcggcg tgcgggcaaa
    15421 agcgcacaac attcccagcc tgaaactgga atacggcacg ctggtgccga tgcgctacat
    15481 gaatgaagac aagcacttca aagtggtctc catttcggct ttctgcacgg ttcacgattt
    15541 tgccgacagc cgcaagctgg gcgaagcgat tctgaaagcg atcgaacagt acgacggcac
    15601 cgtggcggtc cttgccagcg gttcgttatc gcaccgcttt attgacgatc agcgtgcgga
    15661 agaagggatg aacagctaca cccgcgagtt cgaccgccag atggacgagc gcgtggtgaa
    15721 gttgtggcgc gaaggccagt tcaaagagtt ctgcaatatg ctgccggagt acgccgacta
    15781 ctgctacggc gaaggcaata tgcacgacac ggtgatgctg ctggggatgc tcggctggga
    15841 taaatacgac ggcaaggtgg agtttatcac cgagctattc ccaagctctg gcaccggtca
    15901 ggttaacgct gttttcccgc ttcccgcgta aggacgtttt atgccgcact ttatcgttaa
    15961 atgcagtgac aacatccgcg aagaagccga cctgccgggg ttgttcgcca aagtgaatcc
    16021 gacgctggca gccacgggta tttttccgct ggcgggtatt cgcagccgcg tgcattgggt
    16081 cgatacctgg cagatggccg acgggcagcg tgattatgcc ttcgtgcata tgacgttgaa
    16141 aatcggcgca ggtcgcagcc tggaaagccg ccagcaggcg ggtgaaatgc tgtttgaact
    16201 gattaaaacg cacttcgccg ccctgatgga gagccgcctg ctggcgttgt cgtttgagat
    16261 tgaagagctg catccgacgc tgaattttaa acaaaacaac gtgcacgcat tgtttaagta
    16321 gcgcgcagat tgcccggtgg cgctgcgctt accgggccta caaaacccca aaccgtacac
    16381 cgtaggccgg ataaggcgca gccgcatccg gcaatgccac aggatatcgc tatgttcgat
    16441 aaacacaccc acaccctgat cgcccagcgt ctggatcagg cagaaaaaca gcgcgaacag
    16501 atccgcgcga tctcgctgga ttacccggag atcaccatcg aagacgctta cgcggtgcag
    16561 cgtgaatggg ttcgcctgaa aatcgccgaa ggtcgcacgc tgaaaggcca caaaatcggc
    16621 ctgacctcga aagcgatgca ggcaagctcg cagatcagcg aaccggatta cggcgcgctg
    16681 ctggatgaca tgttcttcca cgatggcagc gatatcccga ccgatcgctt tatcgtgccg
    16741 cgcattgaag tggagctggc tttcgtgctg gcaaaaccgc tgcgtgggcc aaactgcacg
    16801 ctgttcgacg tctacaacgc cacggactac gtgatcccgg cgctggagct gatcgacgct
    16861 cgctgccaca acatcgatcc ggaaacccag cgcccgcgca aagtgttcga caccatttct
    16921 gataacgccg ccaacgccgg ggtgatcctc ggcggtcgtc cgatcaaacc agacgaactg
    16981 gatctgcgtt ggatctccgc cctgatgtat cgcaatggcg tgattgaaga aaccggcgtc
    17041 gccgctggcg tgctgaatca tccggcaaac ggcgtggcct ggctggcgaa caaactcgca
    17101 ccgtatgacg tacaactgga agccgggcaa atcattctcg gtggttcgtt cacccgcccg
    17161 gttccggcgc gtaagggcga caccttccac gtcgattacg gcaacatggg ttccattagc
    17221 tgccgctttg tttaaggaga gaacgatgga aaacagcttt aaagcggcgc tgaaagcagg
    17281 ccgtccgcag atcggattat ggctggggct gagtagcagc tacagcgcag agttactggc
    17341 cggagcagga ttcgactggt tgttgatcga cggtgagcac acgccgaata acgtgcaaac
    17401 cgtgctcacc cagctacagg cgattgcgcc ctaccccagc cagccggtag tacgtccgtc
    17461 gtggaacgat ccggtgcaaa tcaaacaact gctggacgtc ggcacacaaa ccttgctggt
    17521 gccgatggta caaaacgctg acgaagcccg tgaagcggta cgcgccaccc gttatccccc
    17581 cgccggtatt cgcggtgtgg gcagtgcgct ggcccgcgcc tcgcgctgga atcgtattcc
    17641 tgattacctg caaaaagcca acgatcaaat gtgcgtgctg gtgcagatcg aaacgcgtga
    17701 ggcaatgaag aacttaccgc agattctgga cgtggaaggc gtcgacggcg tgtttatcgg
    17761 cccggcggat ctgagcgccg atatgggtta tgccggtaat ccgcagcacc cggaagtaca
    17821 ggccgccatt gagcaggcga tcgtgcagat ccgcgaagcg ggcaaagcgc cggggatcct
    17881 gatcgccaat gagcaactgg caaaacgcta tctggaactg ggcgcgctgt ttgtcgccgt
    17941 cggcgttgac accaccctgc tcgcccgcgc cgccgaagcg ctggcagcac ggtttggtgc
    18001 gcaggccacc gccgtgaagc ccggcgtgta ttaatgccgg atgcgctgcg cttatccggc
    18061 ctacactcgc accaaaccgt aggccggata aggcgtttac gccgcatccg gcaaaaagcc
    18121 gtaccaaatc gcggaccgga taaggcgtta cgccgcatcc gccataaacc ttgtcgtacc
    18181 ctacaaaaat cccattagag gaagaaaaat gagcgacacc tcacctgcca taccggagag
    18241 tatcgatccg gcgaatcagc ataaagcgct gactgccgga caacaggcgg ttattaagaa
    18301 gctatttcgc cgcctgagcg tctttctgtt cgtgctgttt atcttctcgt tccttgatcg
    18361 catcaacatc ggctttgccg gactcacgat gggacgcgac ctcggtctga gcgccaccat
    18421 gtttggcctc gctaccaccc tgttctatgc cgcttatgtc atcttcggca ttcccagcaa
    18481 cattatgctg agtattgtcg gtgcgcggcg ctggatcgcc accatcatgg tgctctgggg
    18541 catcgcctct actgccacca tgtttgccac tggccccacc agcttgtacg tactgcgtat
    18601 actggttggc attaccgaag ccggctttct gcctggtatt ctgctgtatt taaccttctg
    18661 gtttccggcc tatttccgcg cccgtgccaa cgccttgttt atggtagcaa tgccggtaac
    18721 gacagcgttg ggctcgatcg tttccggcta cattttgtcg ctggatggcg tcatggcatt
    18781 aaaaggctgg cagtggctgt ttttgctgga aggcttcccg tcggtattac tcggcgtcat
    18841 ggtgtggttc tggcttgatg actcaccgga caaagctaag tggctgacga aagaagacaa
    18901 aaaatgcctg caagagatga tggataacga tcgtctgacg ctggttcagc cagagggagc
    18961 catcagccac cacgccatgc aacaacgcag tatgtggcgg gagatcttca ctccggtggt
    19021 gatgatgtat accctggcgt atttctgcct gaccaacaca cttagtgcga tcagcatctg
    19081 gacaccgcag atcctgcaaa gttttaatca gggcagcagt aatatcacca tcggcatgct
    19141 ggccgccgta ccgcagattt gtaccattct cgggatggtc tactggagcc gtcactcaga
    19201 tcgccgccag gaacgaaggc atcacaccgc ccttccttat ttgttcgctg ccgcaggttg
    19261 gttactggct tcggcaactg atcacaacat gatccagatg ctggggatca ttatggcttc
    19321 gaccggatca ttcagcgcaa tggcgatttt ctggacaaca ccggatcagt ccatcagcct
    19381 gcgggcacga gcgatcggta ttgcggtgat caacgccact ggcaacattg gttcagcgtt
    19441 aagtccgttt atgatcggct ggttgaaaga tttgaccggc agctttaaca gtggattgtg
    19501 gtttgttgcc gcgctgctgg tgattggtgc ggggattatc tgggcgattc caatgcagtc
    19561 ctcccgtccg cgagcgaccc tgtaaggaac gacgatgtgt gaccgtcaga ttgccaatat
    19621 tgatatcagc aaagagtacg atgaaagcct gggcacggac gatgtgcatt atcagtcgtt
    19681 cgcccgcatg gcggcatttt ttggccgcca tatgctgcca catcgccacg aacagtactt
    19741 tcagatgcat ttcctcaata gcggacagat tgagctacag cttgacgatc atcgctactc
    19801 ggtggaagcg cccctgtttg tcctgacgcc gccgtca
//
LOCUS       DAEEKQ010000068        19800 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.068, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000068 DAEEKQ010000000
VERSION     DAEEKQ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19800
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.068"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(84..290)
                     /locus_tag="KLK57_002300"
     CDS             complement(84..290)
                     /locus_tag="KLK57_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HBE0892657.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            603..692
                     /gene="kdpF"
                     /locus_tag="KLK57_002301"
     CDS             603..692
                     /gene="kdpF"
                     /locus_tag="KLK57_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HBE0892658.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            692..2365
                     /gene="kdpA"
                     /locus_tag="KLK57_002302"
     CDS             692..2365
                     /gene="kdpA"
                     /locus_tag="KLK57_002302"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HBE0892659.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2388..4436
                     /gene="kdpB"
                     /locus_tag="KLK57_002303"
     CDS             2388..4436
                     /gene="kdpB"
                     /locus_tag="KLK57_002303"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HBE0892660.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHIEANGGHFPADVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            4445..5017
                     /gene="kdpC"
                     /locus_tag="KLK57_002304"
     CDS             4445..5017
                     /gene="kdpC"
                     /locus_tag="KLK57_002304"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HBE0892661.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKLIAARV
                     AALRAANPDASASIPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5010..7694
                     /gene="kdpD"
                     /locus_tag="KLK57_002305"
     CDS             5010..7694
                     /gene="kdpD"
                     /locus_tag="KLK57_002305"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HBE0892662.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRLAYRGRHICEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPSEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANALERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHFEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7691..8368
                     /gene="kdpE"
                     /locus_tag="KLK57_002306"
     CDS             7691..8368
                     /gene="kdpE"
                     /locus_tag="KLK57_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HBE0892663.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVFEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSPVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFML"
     gene            8643..8747
                     /locus_tag="KLK57_002307"
     CDS             8643..8747
                     /locus_tag="KLK57_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HBE0892664.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9058..11256
                     /gene="speF"
                     /locus_tag="KLK57_002308"
     CDS             9058..11256
                     /gene="speF"
                     /locus_tag="KLK57_002308"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
                     /protein_id="HBE0892665.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGETLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELIAEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNARQVVDKIGHLCDYILFDSAWVGYEQFIPMMADCS
                     PLLLDLNENDPGILVTQSVHKQQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMH
                     ASTSPFYPLFAALDINAKMHEGVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELV
                     DGKPWQSYETAQIAVDLRFFQFVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNG
                     EYEAFGVPATILANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLL
                     ESDAPLAEVLPSIYKQHEERYAGYTLRQLCQEMHDLYARHNVKQLQKEMFRKAHFPHV
                     SMNPQEANYAYLRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFS
                     ALEEGINLLPGFAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            11253..12572
                     /gene="potE"
                     /locus_tag="KLK57_002309"
     CDS             11253..12572
                     /gene="potE"
                     /locus_tag="KLK57_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HBE0892666.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGVIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12628..14268)
                     /gene="pgm"
                     /locus_tag="KLK57_002310"
     CDS             complement(12628..14268)
                     /gene="pgm"
                     /locus_tag="KLK57_002310"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HBE0892667.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQQFVEGLADI
                     VDMAAIQKTGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14294..14839)
                     /gene="seqA"
                     /locus_tag="KLK57_002311"
     CDS             complement(14294..14839)
                     /gene="seqA"
                     /locus_tag="KLK57_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HBE0892668.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15024..15788
                     /gene="ybfF"
                     /locus_tag="KLK57_002312"
     CDS             15024..15788
                     /gene="ybfF"
                     /locus_tag="KLK57_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE0892669.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     RDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMHQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            15928..16221
                     /gene="ybfE"
                     /locus_tag="KLK57_002313"
     CDS             15928..16221
                     /gene="ybfE"
                     /locus_tag="KLK57_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HBE0892670.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16361..16891
                     /gene="fldA"
                     /locus_tag="KLK57_002314"
     CDS             16361..16891
                     /gene="fldA"
                     /locus_tag="KLK57_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HBE0892671.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17101..17187
                     /gene="uof"
                     /locus_tag="KLK57_002315"
     CDS             17101..17187
                     /gene="uof"
                     /locus_tag="KLK57_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HBE0892672.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17180..17626
                     /gene="fur"
                     /locus_tag="KLK57_002316"
     CDS             17180..17626
                     /gene="fur"
                     /locus_tag="KLK57_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HBE0892673.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(17711..18037)
                     /locus_tag="KLK57_002317"
     CDS             complement(17711..18037)
                     /locus_tag="KLK57_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892674.1"
                     /translation="MKKLILIAIMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQHFADQESVRVLDYQQYLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18087..19493)
                     /gene="chiP"
                     /locus_tag="KLK57_002318"
                     /pseudo
     CDS             complement(18087..19493)
                     /gene="chiP"
                     /locus_tag="KLK57_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
BASE COUNT         4543 a         4984 c         5264 g         5009 t
ORIGIN      
        1 aaatattcct ctgaacatct tcctgataca aaaaaccgcc agcgtttccg ctggcggttt
       61 tcaatgtatt cagaactcag caatcagtaa aaatcaccgg ttgccttttc ggcctgatcc
      121 atccacaccg gtttatcgct ggttttcgac cagacgcggt gcaggtagct gtaaaagcgg
      181 gcgcggtctt tccagaaaag catgaaaggc agcgccagaa cgcccgctgc aaccgcatat
      241 gtacggcgta aaaagataag ccatgcagga tattctctgt agagttccat acttttctcc
      301 cccttttgta tctacccggt gaatagcacc ggaaaaatga atttgtttat ctgatgaaaa
      361 tagtaccgcc ttttgtgtaa ttttactact catccgacca cttatttttg cttattgatg
      421 gtttatttac attcactctg taattaagtt acacaaaagt taaattaata ctaaacatta
      481 gttaaatcat ggcttttgcc atttttatac tttttttaca ccccgcccgc agatttttgc
      541 gaaatctttg cagccagaat tctactcttc cggtatcact tttaggccac tggaggtgca
      601 ctgtgagtgc aggcgtgata gccggcgtgt tgctggtgtt tttattactg ggttatttgg
      661 tttatgccct gatcaatgcg gaggcgttct gatggctgcg caagggttct tactgattgc
      721 cacgttttta ctggtattaa tggtgctggc gcgtccttta ggcagcgggc tggcgcggct
      781 gattaatgac attcctcttc caggtacagc gggcgttgaa cgtatactct ttcgtttacc
      841 tggcgtctct gaccatgaga tgaactggaa gcaatatctt tgtgccattc tcggcctgaa
      901 catgctgggg ctggcggtgc tgttttttat gttgctcggt cagcactatc tgccgcttaa
      961 tccacagcag ttgccggggc tgtcgtggga tctggcgctg aataccgccg tcagctttgt
     1021 cacgaatacc aactggcaat cttatagcgg tgaaaccacg ttgagctatt tcagccagat
     1081 ggcaggctta acggtacaaa actttctttc tgccgccagc gggattgcgg tgatttttgc
     1141 cttcatccgt gcgtttactc gccagagcat gagcacactc gggaatgcct gggtcgatct
     1201 gctacgcatc acgttatggg tgctagtccc tgtggcgttg ttgatcgcac tgttttttat
     1261 tcaacaaggt gcgctgcaaa actttctgcc ctatcaggca gtgaataccg ttgaaggagc
     1321 gcaacagctg ttacccatgg gaccagtagc ttctcaggaa gcgatcaaga tgctcggtac
     1381 taacggcggt ggcttcttta atgccaactc gtcgcatccg tttgaaaacc caaccgcact
     1441 gaccaacttc gtgcagatgc tggcgatctt cttgatccca acagcgctgt gctttgcctt
     1501 tggtgaagtg acgggcgatc gccgccaggg gcgcatgttg ctgtgggcga tgtcagtgat
     1561 ttttgtcatc tgcgtaggcg tggttatgtg ggcagaagtt cagggtaatc ctcatctgct
     1621 ggcgctcggc gcagacagca gcatcaatat ggaaggtaaa gagagccgtt tcggcgtgct
     1681 ggtcagtagc ctgtttgcgg tcgtgactac ggcggcgtcc tgtggcgcgg tgattgcgat
     1741 gcatgattcg tttaccgctc tcggcggcat ggtgccgatg tggctaatgc aaattggtga
     1801 agtggtgttc ggcggtgtcg gttctggtct ttacggcatg atgctgtttg tcctgctggc
     1861 ggtgtttatt gccgggctga tgattggtcg tacaccggaa tatctgggta aaaaaatcga
     1921 cgtacgcgag atgaaactga ctgcactggc gattctggtt actccaacac tggtactgat
     1981 gggcgcggcg ttggcgatga tgaccgacgc cggacgtagc gccatgctta accctggccc
     2041 gcatggtttt agcgaagtgc tgtacgccgt gtcgtccgcc gctaacaaca acggcagcgc
     2101 ctttgccgga ttaagcgcca actctccgtt ctggaactgt ttactggcgt tctgcatgtt
     2161 tgtcggtcgc ttcggggtga ttatcccggt gatggcaatt gccggttcgc tggtgagtaa
     2221 aaagagccaa cccgccagct ccggcacgct gccaacgcac ggcccgctgt ttgttggcct
     2281 gttaatcggc accgtgttgc tggttggcgc actgaccttt atccctgccc tggcgcttgg
     2341 tccggtggcg gaatatctct cctgatgata ttgagcgagc actgaatatg agtcgtaaac
     2401 aactggcgct attcgaacca acacttgtcg ttcaggcgct gaaagaagcg gtgaaaaaat
     2461 taaacccgca ggcgcaatgg cgcaatccgg tgatgtttat cgtctggatc ggcagtctgc
     2521 tgaccacctg tattagcatc gcgatggcaa gcggtgcgat gcctggcaat gcgctgttta
     2581 gcgcggcaat tagcggctgg ctgtgggtca ccgtactgtt cgctaatttc gccgaggcgc
     2641 tggcagaagg ccgcagtaaa gcgcaggcca acagtcttaa aggggtgaaa aaaactgcct
     2701 ttgcccgcaa gctgcgtgag ccgaaatatg gcgctgcggc ggacaaagtt cctgccgatc
     2761 aactgcgtaa aggtgacatc gtgctggtag aagctggcga tattatcccc tgcgatggtg
     2821 aagtcattga aggcggcgca tcggtcgatg aaagcgccat caccggggaa tcggcaccgg
     2881 tgatccgtga atccggcggc gattttgcct ccgtcaccgg cggcacgcgt attctttctg
     2941 actggctggt gattgagtgt agcgttaacc ccggtgagac atttctggat cggatgatcg
     3001 cgatggtgga aggcgcacag cgacgcaaaa caccgaacga gattgccctg actatcctgc
     3061 tgattgctct gaccatcgtc tttttactgg caaccgccac gctgtggccg ttttccgcgt
     3121 ggggcggtaa tgccgtgagt gtaacggtac tggtggcgct gctggtctgt ctgatcccaa
     3181 ccactattgg cggcctgttg tcagcgatcg gcgtcgccgg gatgagtcgg atgctgggcg
     3241 cgaatgtgat tgccaccagc ggacgtgcag ttgaagcggc aggtgacgtt gacgttctgc
     3301 tactggataa aaccggcacc atcacactcg gtaaccgtca ggcgtcggag tttatccccg
     3361 cgcagggcgt ggatgaaaaa acgctggctg acgccgcaca actggcttcg ctggctgatg
     3421 aaacgccgga aggtcgcagt attgtgatcc tcgccaagca acgttttaac ctgcgcgagc
     3481 gcgatgtgca gtcgctccat gccacctttg taccgtttac tgcgcaaagc cggatgagcg
     3541 ggatcaacat cgacaaccgc atgatccgta aaggttctgt cgatgccatt cgccgccata
     3601 ttgaggctaa cggcggtcac ttccctgccg atgttgatca aaaagtcgat caggttgcgc
     3661 gtcagggagc cacgccgctg gtggtggtgg aaggttctcg tgtgctgggc gttattgcgc
     3721 tgaaagatat cgtcaaaggc ggtattaaag agcgcttcgc ccagctgcgc aaaatgggca
     3781 ttaaaacggt gatgattacc ggcgataacc gtctgactgc cgccgcgata gctgcggaag
     3841 cgggtgtcga tgattttctc gccgaagcaa caccggaggc caagctggca ttgatccgtc
     3901 agtatcaggc ggaaggtcgt ttggtagcga tgaccggcga cggcaccaac gatgctccgg
     3961 cgctggcgca ggcagatgtc gcggtggcga tgaactccgg cacccaggcg gcgaaagagg
     4021 cgggcaatat ggtggatctc gactctaacc cgaccaagtt gatcgaggtg gtgcacattg
     4081 gcaaacagat gctgatgacc cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg
     4141 cgaaatactt cgccattatt ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc
     4201 tgaacatcat gcgcctgcat tcgcccgact ccgccattct cagtgcggtc attttcaacg
     4261 ccttgattat cgtctttttg attcctctgg cgttaaaagg cgtgagttat aaaccgctta
     4321 ccgcttctgc catgttgcgc cgtaacttat ggatttacgg tctgggtggg ctgctggtgc
     4381 cgtttattgg tatcaaagtc attgatttac tgctgaccgt ttgcggtctg gtgtgaggtt
     4441 taccatgagt ggattacgtc cggcattatc gacatttctc tttctgttat tgattactgg
     4501 cggcgtttac ccgctgctga ccaccgcact ggggcaatgg tggtttccct ggcaggccaa
     4561 cggttcgttg atccgtgaag gtgatacggt gcgtggctcg gcattaatcg ggcagaattt
     4621 taccggcaac ggctattttc atggtcgccc gtcagcaacg gctgaaatgc cctataatcc
     4681 acaggcttct ggcgggagca atctggcggt cagtaaccct gagctggata aactaatagc
     4741 cgcacgcgtt gctgcattac gggccgctaa cccggatgcc agcgcgagca ttccggttga
     4801 actggtgacg gcatcggcaa gcgggctgga caataatatc accccgcaag cggcggcctg
     4861 gcagatccca cgcatcgcga aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat
     4921 cgcaaaatac agccaacaac cgctggtgaa atatatcggc cagccggtcg tcaacattgt
     4981 cgaactcaat ctggcgctgg ataagcttga tgaataacga acccttacgt cccgaccccg
     5041 atcgtctgct ggaacaaact gccgcgccgc atcgggggaa gctgaaagtt ttcttcggtg
     5101 cctgtgcagg cgtcgggaag acctgggcga tgctggcaga agcccagcga ctgcgggcgc
     5161 aagggctgga tattgtggtt ggcgtggtag aaacccacgg gcgaaaagat accgccgcaa
     5221 tgctggaagg gctggctgtt ctgccgccaa aacgcctggc gtaccgtggg cggcacatct
     5281 gcgagtttga cctcgatgcc gccctcgccc gccgtccggc gctgatctta atggacgaac
     5341 tggcgcacag taatgcgcca ggttctcgtc accctaaacg ctggcaggac atcgaagagc
     5401 tgctggaagc aggcattgat gttttcacta ccgtcaacgt tcagcatctg gaaagtctga
     5461 atgatgtggt cagcggcgtc accggaattc aggtacggga aaccgtgccc gatccttttt
     5521 tcgatgccgc cgacgacgtg gttctggtgg acttgccccc ggacgatctg cgccagcggc
     5581 tgaaagaagg caaagtctat attgccgggc aggcggagcg cgccattgaa cattttttcc
     5641 gcaaaggcaa tctgatcgcc ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg
     5701 atgagcaaat gcgcgcctgg cgggggcatc ctagcgaaga gaaagtctgg cacacgcgcg
     5761 acgcgatcct tttatgcatc ggccataaca ccggtagtga gaagctggtc cgcgcggcgg
     5821 cgcggctggc gtcacggctg ggtagcgtct ggcacgcggt gtatgttgaa acccctgccc
     5881 tgcatcgctt accggagaaa aaacgtcgtg caattctcag cgccttacgt ctggcgcagg
     5941 aactgggcgc ggagacggca acactttctg atccagcgga agagaaagcg gtagtgcgtt
     6001 atgcccgtga acataatctc ggcaagatta ttctcggtcg cccggcctcg cgccgctggt
     6061 ggcgtcggga aacgtttgct gaccgactgg cgcgcatcgc ccccgatctc gatcaggtgc
     6121 tggtcgcgct tgatgaacca cccgcccgca cgattaacaa cgcgccggat agccgctctt
     6181 ttaaagacaa gtggcgggta cagattcagg gatgcgtggt tgccgccgcg ttatgcgccg
     6241 ttatcacctt aattgccatg cagtggctga tggcgtttga tgccgccaac ctggtgatgc
     6301 tgtatctgct tggcgtggtg gtggtggcgc tattttatgg acgctggcct tcggtggttg
     6361 ccaccgtcat taatgtggtg agtttcgatc tcttttttat cgccccacgc ggcacgctcg
     6421 ccgtctctga tgtgcaatat ctgctgacct tcgcggtgat gttaaccgtc gggctggtga
     6481 tcgggaacct tactgctggc gtgcgttatc aggcgcgggt agcccgttac cgcgagcaac
     6541 gcacacggca cttatatgaa atgtcgaaag ctctggcggt gggccgcagt ccgcaggaca
     6601 tcgccgccac cagcgaacaa tttattgcct ccacgtttca tgcccgcagt caggtgttgt
     6661 tgcccgatga caacggtaaa ttgcagccgt taacacatcc gcaaggaatg acgccatggg
     6721 acgatgccat cgcgcagtgg agttttgata aaggcctgcc tgcgggcgcg ggcaccgaca
     6781 cgttacccgg tgtaccgtac cagattttgc cgctaaaaag cggcgagaaa acctacgggc
     6841 tggtggtggt ggagccgggg aatctgcgcc agttgatgat cccggaacag cagcgcctgc
     6901 tggagacgtt tacgctgtta gtcgccaatg cccttgagcg gctgacgcta accgccagcg
     6961 aagaacaggc gcggatggca agcgaacgtg aacagatccg caacgccctg ctggcagcgc
     7021 tttcgcatga tttacgcacg ccgcttacgg tgctgtttgg tcaggcagaa atcttaacgc
     7081 tcgatctggc aagcgaagga tcaccccacg cccgccaggc cagcgagatt cgtcagcatg
     7141 tgctgaacac tacccgactg gtgaataatc tactggatat ggcgcgaatt cagtccggcg
     7201 gctttaattt gaagaaagag tggttaacgc tggaagaagt agtcggcagc gcgctgcaaa
     7261 tgctggaacc gggtttatcg tcgcccatca atctttctct gccagaaccg ctgaccttaa
     7321 tccacgttga cgggccactc tttgaacggg tgctgattaa tctgctggag aacgcggtga
     7381 aatatgcggg tgcgcaggcc gaaattggta tcgatgccca ctttgagggc gaaaatctac
     7441 aactggatgt ctgggataac ggccccggtc ttccgccagg ccaggagcag acgatatttg
     7501 ataagtttgc tcgcgggaat aaagagtcgg cggtgccggg tgtcgggctt gggttggcaa
     7561 tttgtcgggc gattgtggat gtacacggcg gcaccattac cgcgttcaac cgaccggaag
     7621 gtggtgcctg ttttcgtgtt acacttcccc agcaaaccgc tcctgaactt gaagactttc
     7681 atgaggatat gtgacaaacg ttctgattgt tgaagatgaa caggctattc gtcgctttct
     7741 gcgcacggcg ctggagggcg acgggatgcg cgtctttgag gccgaaactc tgcaacgcgg
     7801 cttgctggaa gcggcaaccc gtaagccaga tttgattatt ctcgatctcg gcctgcccga
     7861 tggtgatggg attgagttta tccgcgacct gcgccagtgg agcccggtgc cggtgattgt
     7921 gctttccgca cgcagcgaag agagcgacaa gatcgccgcg ctggatgccg gagcggatga
     7981 ttatctgagt aagccgtttg gcattggcga attgcaggcc cgtctgcgcg tcgctttacg
     8041 ccgccactcc gccaccgccg cgcccgatcc gctggtgaaa ttttccgatg ttaccgtcga
     8101 tttagccgcc cgcgtgattc accggggtga ggaagaggtg catctcacac caattgagtt
     8161 ccgcctgctg gcggtgctgc tcaacaatgc cggaaaagta ctcacccagc gccagctcct
     8221 taaccaggtg tgggggccaa acgcggtcga acacagtcac tatttgcgta tttatatggg
     8281 acatctgcga caaaaactgg aacaggatcc ggcccggcct cgccacttca ttactgaaac
     8341 cggtattgga tatcggttta tgctttgaat tgttattcaa taaattcgct taaatagtga
     8401 ttaatttatt attaaacctg cgccgaatat tattatttgg caggtttaat ttcttaacgc
     8461 aaatataaaa cacgaaatta caacatttaa gtaacaaaac agcatctaat atggcgatat
     8521 tcataattaa tgatttatta tctggggtat gatcgttttt tgttgatctt cttcacagat
     8581 tatagccatt tcatggatag aataactcta ccttcaactg acacagcaag aggtaaaggt
     8641 aaatggaaaa taataaccgc ttaatgcctc atataaggcg gacaacccat atcatgaagt
     8701 ttgcccatcg taatagtttt gactttcatt tctttaatgc ccgctagtct tctgactaaa
     8761 gggcacccca acgtacaggt ctccctgact ttaagcatta caggttaata cctgtattcc
     8821 tcggtgctca tatactgcta acccttttaa actctaaata attcgagtcg cagcacttgc
     8881 aacttgaggt atgacgagta tagccagtta ccgggctggt ctgggttatt gcatctgcaa
     8941 aaagcaaact actgatttat ttatcagcgg tggagctttg ctttttttcc ggcgtgatcg
     9001 atttctcctt tgagaaattg aggacctgct attacctgaa ataaagagat gaataaaatg
     9061 tcagaattaa aaattgccgt tagccgttct tgcccggatt gtttttccac tcatcgtgca
     9121 tgcgtgaata ttgacgaaag taattatatt gacgttgccg ccattatttt atcagtcaat
     9181 gatgttgaac gtggaaaact cgatgaaata gacgctactg gctatggcat tcctgttttt
     9241 attgcaacgg aaaatgaaga acgtgtacca gcagaatatc tgccacgtat ttctggtgtc
     9301 ttcgagcatt gcgaatcacg gaaagagttt tatggacgtc agttagaaac cgctgctagc
     9361 cattatgaaa cgcaactgcg cccgcccttc ttccgcgcac tggtcgatta tgtcaatcag
     9421 ggcaatagcg cttttgactg cccgggtcat cagggaggtg agtttttccg tcgtcatccg
     9481 gcagggaatc agttcgtgga gtacttcgga gaaacgctgt tccgttccga cttatgcaac
     9541 gcagacgtag caatgggcga tctgctgatt cacgaaggcg cgccatgcat tgcacagcaa
     9601 catgcggcaa aagtgtttaa tgccgataaa acatacttcg ttctaaatgg cacttcatct
     9661 tctaacaaag tggttttaaa cgccctgcta acaccgggtg atctggtgct gtttgatcgc
     9721 aataaccaca aatctaacca ccacggagcg ttgctacagg ctggtgcaac accggtttat
     9781 ctggaaacgg cacgtaaccc gtatggcttt atcggtggca ttgatgcgca ctgttttgaa
     9841 gaaagttacc tgcgtgagct gatcgcggaa gtcgcaccgc agcgggcaaa agaggctcgt
     9901 cctttccgcc tcgctgtgat tcagttaggc acctacgacg gtacgattta taacgcccgc
     9961 caggtggtgg ataaaattgg tcatctgtgt gactacattc tgtttgactc cgcatgggtt
    10021 ggctatgagc aatttatccc gatgatggct gactgttcgc cgctgttgct ggatcttaat
    10081 gagaacgatc cgggtattct ggttacgcaa tccgtacata aacagcaggc tggtttttct
    10141 caaacttcac aaatccataa aaaagacagc cacatcaaag ggcaacagcg ttatgtaccg
    10201 cacaaacgca tgaacaacgc ctttatgatg cacgcctcca ccagcccgtt ctatccgctg
    10261 tttgccgcac tggatatcaa cgccaaaatg catgaaggtg tcagcggtcg taatatgtgg
    10321 atggattgtg tggtaaatgg cattaatgcc cgcaaactga tcctcgataa ctgtcagcat
    10381 attcgtccgt tcgtacctga actggtggat ggtaaaccct ggcagtcgta tgaaacagcg
    10441 caaattgcgg ttgatctgcg cttcttccag tttgtaccag gggaacactg gcattctttt
    10501 gaaggctatg cagagaatca atactttgtc gatccttgca aactgttgct gacaacccca
    10561 ggtattgatg cacgtaacgg cgaatatgaa gcgttcggtg tacccgcgac gattcttgct
    10621 aacttcctgc gcgaaaatgg cgtagtgccg gaaaaatgcg atcttaactc catcctcttc
    10681 ctgctgactc cggcagaaga tatggccaaa cttcagcaac ttgttgccct gctggtacgc
    10741 ttcgaaaaac tgcttgagtc cgacgcgcca ttagcagaag tgctgccttc catctacaaa
    10801 cagcatgaag agcgctacgc cggttatacc ctgcgtcagt tgtgccagga aatgcatgat
    10861 ttgtatgccc gccacaacgt gaaacaactg caaaaagaga tgttccgcaa agcgcacttc
    10921 ccacacgtca gcatgaatcc gcaagaagcc aactacgcct atttacgcgg tgaagtggag
    10981 ctggttcgtc tgccggatgc cgaaggtcgt atcgctgccg aaggggcgct tccttatcct
    11041 ccgggtgtgc tgtgtgttgt tccgggtgaa atctggggtg gtgctgtttt gcgttacttc
    11101 agcgctctgg aagaagggat caacctgctg ccaggttttg caccggagct gcagggtgtc
    11161 tatatcgaag aacatgatgg tcgtaagcaa gtttggtgct atgtcatcaa gcctcgtgat
    11221 gcgcaaagca ccctgttgaa aggggaaaaa ttatgagtca ggctaaatcg aacaaaatgg
    11281 gcgtcgttca gttaaccata ctgacgatgg tcaacatgat gggctccggt atcatcatgc
    11341 tgccgacaaa gcttgccgaa gttgggacaa tttcaattat ctcctggctg gtgacagccg
    11401 taggctcaat ggcactggca tgggcattcg caaaatgcgg tatgttcagc cgtaaatcag
    11461 gtggtatggg cggttatgcc gagtatgctt tcggtaaatc cggtaacttt atggcgaact
    11521 atacctacgg cgtctcactg ctgattgcta acgtcgcgat tgctatttcg gcggttggtt
    11581 acggcaccga actgctcggc gcgagtttgt cgccagtgca gattggtctt gcgaccatcg
    11641 gggtgctgtg gatttgtacc gtggctaact ttggtggtgc gcgcattacc gggcaaatca
    11701 gtagcattac cgtgtggggg gtcattattc cggtggtagg cctgtgcatt atcggctggt
    11761 tctggtttag cccgacgctt tacgttgatt cctggaatcc gcatcatgca ccgttcttca
    11821 gtgcagtagg ttcttccatc gctatgacgc tgtgggcttt tctcggtctg gagtctgcgt
    11881 gtgcgaatac tgatgtagtg gaaaacccgg aacgtaatgt gccaatcgcg gtactcggcg
    11941 gtacgttagg tgcggcggtg atttatatcg tctccaccaa cgtgattgca gggattgtgc
    12001 ctaatatgga gctggcaaat tcaacggcac catttggcct ggccttcgca cagatgttca
    12061 caccagaagt gggtaaagtc attatggcgc tgatggtgat gtcctgctgc ggttcgctgc
    12121 ttggctggca gttcaccatt gcccaggtgt ttaagtcttc atctgatgaa ggctacttcc
    12181 ctaaaatttt ctcccgtgta accaaagtgg atgcaccggt gcagggaatg ttgaccattg
    12241 tgattatcca gagcgggttg gcgctgatga cgattagccc gtcgctaaac agtcagttca
    12301 acgtgctggt taacctggcc gtggtgacca atatcattcc gtatattctg tcaatggcgg
    12361 cattagtcat tattcagaag gtcgctaatg tgcccccctc aaaagcgaaa gttgcaaact
    12421 ttgttgcttt tgttggcgcg atgtatagct tttatgcgct gtactcatcc ggggaagaag
    12481 ccatgctgta cggttccatc gtgaccttcc ttggttggac actgtatggt ctggtatcac
    12541 cacgctttga actgaaaaat aaacacggtt aaaaaagcaa agtaaaaaag ggcgatcttg
    12601 cgaccgccct ttttttatta aatgtgttta cgcgtttttc agaacttcgc taacaatctc
    12661 aaccgcttct ttctcaatct gcttgcgatg ttcttcaccg aggaagcttt cgcagtagat
    12721 cttatatgcg tcttccgtgc ctgacggacg cgcggcgaac cagccgttgt cagtcatcac
    12781 tttcagaccg ccgatagaag caccattgcc cggtgccgcc gtcaggcgcg cggtgatcgg
    12841 gtcgcctgcc agggtgctgg cgctcaccat ttccggagac agcttagaca gcgccgcttt
    12901 ttgtgcggaa gtcgcagctg cctgcaaacg gttgtagctc ggcgcaccaa agcgttttgc
    12961 cagttcgttg tagtgttcct gcgggttctt accggtgaca gcggtgattt ccgccgccag
    13021 cagacacatg atgatgccgt ctttgtcggt ggaccacggc gtgccgtcga aacgcaggaa
    13081 ggaagcccct gcgctctctt cgccgccaaa gccgaagctg ccgtcgaaca gaccatcaac
    13141 aaaccatttg aaacctaccg ggacttctac cagcttgcgg cccaagtcat tgaccacacg
    13201 gtcgatcatc gcagaagaaa ccagcgtttt accgacagca acatctttgc cccactgcgg
    13261 acgatgctgg aacaggtagt tgatcgccac cgccaggtag tggttcggat tcatcaaacc
    13321 tgccggagtg acgataccgt gacggtcata atccgggtcg ttagcaaacg ccagatcgaa
    13381 cttatcacgc aatgccagca ggcccgccat cgcacactcg gaggagcagt ccatacggat
    13441 cgcgccgtct ttatcgaggt gcataaagcg gaaggtttga tcgacctgat cgttaacgat
    13501 ggtcaggttg aggttgtaat actcgccaat tcgcttccag tattcgatac cggaaccacc
    13561 cagcggatca acgcccagcg tcaggcccgt tttctgaatc gccgccatat caacgatatc
    13621 ggccagacct tccacaaact gctgcaccag atcctgctct ttcacatgac cggatgccat
    13681 cgcttcgtcg agggagatac gcttcacgcc tttcaggcca tcggccatca gtgcgttggc
    13741 acgatcttcc accactttag tgacgttggt atcagccggg ccaccatttg gcggattgta
    13801 cttgatacca ccatcttccg gcgggttatg ggagggtgta atcacgatac cgtctgccag
    13861 cgggccacct tttttattat gaaccaggat ggcattggaa acggcaggcg tcggagtgaa
    13921 gccattgttt tcctgcacaa tgacatcaac gccgttcgct gccagcactt ccagtacgga
    13981 aatgaacgca ggctcggaca gggcgtgagt atctttaccc acatagcaag ggccagtgat
    14041 gccgtttttc gcacgttctt cagcaattgc ctgagcgatc gccagaatgt gcggctcgtt
    14101 aaagctgtgg cgcgctgcac tgccacggtg accggaagta ccgaatttca ccgcgtgctc
    14161 cgcattccct gcttctggtt tcagtacata atattgcgcc gtcagttggg cgacgttaat
    14221 caaatcactc tgttgtgcag gttggcctgc acgattgtgg attgccattg ctttgtcctt
    14281 tgtctgcaac gttttagata gttccgcaaa ccttctcaat caattccgcc gggaattgca
    14341 tcgactgcat gatgtgttcg atcatgctgc atttacggcc ggtgttggtg ttggtgatca
    14401 cccaatacgg cgtgcctggc acatgtttcg gcttggtctg attaccattt ttcagcagcg
    14461 tttgttcatc tgccgcaaag taaacgcgtg tacgaccgtg caacgattcc gttgcttcag
    14521 caaacgcctg ggcgtcaaga gaatatagtg tagacaacag cagcataaag cgattgaccg
    14581 ctcgcttttg ctctgcgtat tcgtcagaaa gcagaagttc acgcattgcg cgaaccttgt
    14641 ctttaatcgt tttgaccggc ttcgcttcga cgatagcagg tgacgcaacg cgaacctctt
    14701 tcgtcaccgg agcagcaggc tgtgatgcgg cggaaaattt caacatacgc cgtaaaatgt
    14761 cggatgcgct ctcgccgata tgcttagtgt ggctggcaat atagctgtag agttcatcat
    14821 caacttcaat cgttttcatc ttaatccagt acagtgtctt agctgaatac aagtcgccaa
    14881 gagtatatga ataaatcccg gcagcggata gcgtcaagcc tggcttgcag aaaaactcgg
    14941 aataaacctt atttgttgca gccctgtggc acaatggtca acataatacc ctaacccgac
    15001 aacgcgaaaa aaagaacttt gccatgaaat tgaatatccg cgcgcaaact gcacaaaacc
    15061 agcacaataa ttctcccatc gttcttgtcc atggtctgtt tggtagcctc gacaaccttg
    15121 gcgtactggc tcgcgatctg gtaaacgatc acaatatcat ccaggttgat atgcgtaacc
    15181 acggtctttc accgagagat ccggtaatga attacccggc gatggcgcag gatcttgttg
    15241 atactctgga tgcacagcag atcgacaaag caacatttat cggtcactcc atgggcggta
    15301 aagcggtaat ggcacttacg gcactggccc gcgatcgcat cgataaactg gtggcgatcg
    15361 atatcgcacc ggtcgactat cacgtacgcc gtcatgatga gattttcgcg gctatcaacg
    15421 cggtcagtga atcggacgca caaactcgcc agcaagcagc agcaataatg caccagcatc
    15481 ttaatgaaga aggggtgatt cagtttctgt tgaaatcttt tgttgacggg gagtggcgct
    15541 ttaacgtgcc ggtattgtgg gatcagtatc cgcatattgt gggttgggag aaaatcccgg
    15601 catgggatca ccctgccctg tttatccctg gcggcaattc tccgtatgtt agcgagcagt
    15661 accgtgatga tttactggca caatttccac aggcacgggc gcatgtgatt gcaggcgcgg
    15721 gtcactgggt ccatgctgaa aaaccggatg cggtattacg cgctatccgt cgctatctca
    15781 atgattaacg caactgatta aaaacccagc gtccgcgtcg ctttccgcgc gtgggcgttg
    15841 gcgtgactca tcgcgctgat gtattatggc gcgctatcca tccgggcaga ggcctggctg
    15901 attgtttccc ccgaagtcac caagatcatg gccaaagaac aaacggaccg tacgacatta
    15961 gatctgttcg cgcacgagcg tcgaccggga cgaccgaaaa ctaatccgct ttcgcgcgat
    16021 gaacagctgc gtattaataa acgcaaccag ctaaaacgcg acaaagtacg tggccttaag
    16081 cgtgtcgaac tgaagctgaa cgcggaagct gtcgaggcgc tgaacgagct ggcggagtcg
    16141 cgcaatatga gccgtagcga actgatcgaa gagatgttaa tgcagcaact ggcggcgctg
    16201 cgtagtcagg gcatcgttta aatttccact ttcatgtagc acagtgtgca gtcctgctcg
    16261 tttgctgatt ccgcaagact gcctgttctg ctatgattgc ctttatccgt gggcaatttt
    16321 ccacccccat ttcaataagt ttcaagaggt tatttcactc atggctatca ctggcatctt
    16381 tttcggcagc gacaccggta ataccgaaaa tatcgcaaaa atgattcaaa aacagcttgg
    16441 taaagacgtt gccgatgtcc atgacattgc aaaaagcagc aaagaagatc tggaagctta
    16501 tgacattctg ctgctgggca tcccaacctg gtattatggc gaagcgcagt gtgactggga
    16561 tgacttcttc ccgactctcg aagagattga tttcaacggc aaactggttg cgctgtttgg
    16621 ttgtggtgac caggaagatt acgccgaata tttctgcgac gcattgggca ccatccgcga
    16681 catcattgaa ccgcgcggtg caacgatcgt tggtcactgg ccaactgcgg gctatcattt
    16741 cgaagcatca aaaggtctgg ctgatgacga ccactttgtc ggtctggcta tcgacgaaga
    16801 ccgtcagccg gaactgaccg ctgaacgtgt agaaaaatgg gttaaacaga tttctgaaga
    16861 gttgcatctc gacgaaattc tcaatgcctg atgtgatgcg gcgtagactc atgtctacgc
    16921 cgtattaata gataatgcca atcaaaataa ttgctacaaa tttgtaactt ttgctgttgt
    16981 acctgtacaa tgtcccggtg ttcaagtggc cttgccgttg taaatgtaag ctgtgccacg
    17041 tttttattaa caatatttgc cagggacttg tggttttcat ttaggcgtgg caattctata
    17101 atgatacgca ttatctcaag agcaaattct gtcacttctt ctaatgaagt gaaccgctta
    17161 gtaacaggac agattccgca tgactgataa caataccgcc ctaaagaaag ctggcctgaa
    17221 agtaacgctt cctcgtttaa aaatcctaga agttcttcag gaaccggaca accatcacgt
    17281 cagtgcggaa gatttataca aacgtctgat cgatatgggt gaagaaattg gtctggctac
    17341 ggtatatcgc gtactgaacc agtttgacga cgctggtatc gtcacccgcc acaattttga
    17401 aggcggtaaa tccgtatttg aactgacaca gcaacatcac cacgatcacc tgatctgcct
    17461 cgactgcggc aaggttatcg aatttagtga tgattccatc gaagcgcgtc agcgtgaaat
    17521 tgccgcaaaa catggcattc gcctgactaa ccacagtctc tatctttacg gtcactgtgc
    17581 cgaaggcgat tgccgcgaag atgaacatgc gcacgaaggc aaataagcca gcctgaatga
    17641 gaaaaagcca acctgcgggt tggctttttt atgcaaggga aataagaaaa gcgagagtta
    17701 cagctctcac ttatttgtta ttactgcgaa tttcctgcca aactttatca caatcggctt
    17761 tcaccgcctg atcatttccg gtttgcgttg cacgcagata ctgctgataa tccagtacgc
    17821 ggacactttc ctggtcggca aaatgctgat gttgtttctc tttcttcagc acgtttaaca
    17881 cgctctggca ggcttcaatt ttttctggcg aaccttgtgc ggtgttgata caggcgctgt
    17941 atgcctcttt cagacgactg tcttcctgtg gcgcggttga ttgcgcacaa gctaccagcc
    18001 ccgatgccat tatggcgatg agaatcagtt ttttcatagc aaattcctca aacgtgcggc
    18061 aggcacaaaa cctgccgcgt cgggcatcag aagatggtga atggtgcgat taccataaat
    18121 ttcacgtcac gctcatcctg gaagatgttg ccgtaaccac cgccccagct tgggatgtcg
    18181 gagtggttgt cgtattcggt gaagtgcagt ttgaacatcg tgcctttggc gcgaccgtcc
    18241 tgaatggtgt agaccgcatc caggctgtat gcagactctt caatagtccg gtttttgtcg
    18301 tagtacgcat ccggattgct ctgctaggtc gcaggtttag catcccatgc gtaaacgtag
    18361 gaagcgccga tggcgaagcc tggaagattc cagtttttca ggtcatacat cgcaccgaag
    18421 aagaccgctt tttcgccgtt agcgttgaag tcggaacggt tatcccacca gatatccagg
    18481 cggccgttgg aggaagcgta ggttggagtc atacgttgca ggaagtatcc ctgctgaccg
    18541 tcagccttaa cccaggtgcc ttcgaggcgc aaatccacta cgtcagccgc ccggtaacca
    18601 aaggtcaacg cttgcagcca ggcggtgccg tcataaaggt cgttgacgct gcgatcgtca
    18661 actttatcgc gcgtaccata gaactggtag ctggtggtta acgggctacc ggcgatatca
    18721 aatttgtagc tggctttggc aaaatattga tcgatatacc cttccgcctg accaaatgcc
    18781 gcttccagta cgaagttatt tttgaagtcg tatttcgccc caagggagtg cagataatca
    18841 actttggtgg ttttatcgtt ctgataaaac tcatccattt ccagatgcca cggcgctttg
    18901 tattcgttgg tccacatgta ggagaaactc aacgcaccag catcgccgta atcaaaattc
    18961 gccccggctt ccgccccctg ataagtacct ggcataaagc tccagtgcgg cgctaacagc
    19021 gtttgaccag ttggctgaat gtaacctgcc ctcgcccaaa ccggaccgta tttaaatttg
    19081 gccgcagctt tatacaggct tataccgctt ttgtcgccgg accagtcttc gtcataggct
    19141 ttattacttt ttgaaaacgc gatttcgttc gggtgggagc tgtcgccgtt ttccgccatt
    19201 tcaatcgccg taaacgcagc aatatcaagg ccgaacatat cagcagcata accggactga
    19261 aaatcgaggt tggcattcca ggtggagtga gaaaggttgg ttttgtattt gtcgccgtcg
    19321 gtaacatctt tacggtcgcg ttcacgctgc cagtaatata taccgccggt taaggttgaa
    19381 tcgtcgatga atccttcggc gcgagcctcc ggcattgcca taaagcccga cattgctgta
    19441 acaccggcga tagccagcgc cagcgtacta cgtttgccac taaacgtacg catgggttaa
    19501 tcctctttga cgtataaatt gctgcaccaa aggtgaacag cgaaaaaata aaaattaaaa
    19561 aagtagcgtt gagaaaagtc tcgctgacta caggaacaga ctatttttcg cgacgcgaat
    19621 tatcaatctc tttctgtaac cagaatgtaa gaacatgagc aaccgcacat attttgatga
    19681 tttttgttac aaggcttaag tttgcggttg aaaggcttgc agaagaataa aagcgtgaaa
    19741 ttccagcaag atgaaagcgg ttacatttta tttaattatg ttttgttatt ttaaatttat
//
LOCUS       DAEEKQ010000069        19598 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.069, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000069 DAEEKQ010000000
VERSION     DAEEKQ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19598
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.069"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(187..1836)
                     /gene="pgi"
                     /locus_tag="KLK57_002319"
     CDS             complement(187..1836)
                     /gene="pgi"
                     /locus_tag="KLK57_002319"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HBE0892675.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2361..3710
                     /gene="lysC"
                     /locus_tag="KLK57_002320"
     CDS             2361..3710
                     /gene="lysC"
                     /locus_tag="KLK57_002320"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HBE0892676.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHSNLFE"
     gene            3962..4234
                     /locus_tag="KLK57_002321"
     CDS             3962..4234
                     /locus_tag="KLK57_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HBE0892677.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(4367..5239)
                     /gene="rluF"
                     /locus_tag="KLK57_002322"
     CDS             complement(4367..5239)
                     /gene="rluF"
                     /locus_tag="KLK57_002322"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HBE0892678.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            5587..6424
                     /locus_tag="KLK57_002323"
                     /pseudo
     CDS             5587..6424
                     /locus_tag="KLK57_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313031.3"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
     gene            6494..7300
                     /locus_tag="KLK57_002324"
     CDS             6494..7300
                     /locus_tag="KLK57_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0892679.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEVHKGYQFWPTDISSAKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            7310..7717
                     /locus_tag="KLK57_002325"
     CDS             7310..7717
                     /locus_tag="KLK57_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HBE0892680.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGDANVEAVEFVPGENAGDIV
                     AKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATAEEDF"
     gene            7717..8211
                     /locus_tag="KLK57_002326"
                     /pseudo
     CDS             7717..8211
                     /locus_tag="KLK57_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            8277..9074
                     /locus_tag="KLK57_002327"
     CDS             8277..9074
                     /locus_tag="KLK57_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HBE0892681.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALGWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWRKAEPQLQATASTALDQLDD"
     gene            9085..9909
                     /locus_tag="KLK57_002328"
     CDS             9085..9909
                     /locus_tag="KLK57_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HBE0892682.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRRHLVFFNTTPAVCGPVIGVTAAMEEARANGAEIDDGTINGIKVG
                     LMGPLAGVGDPLVWGTLRPITAALGASLALSGNILGPLLFFFIFNAVRLAMKWYGLQL
                     GFRKGVNIVSDMGGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGST
                     VIMTVQNILDQLCPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            9961..11189
                     /gene="sorE"
                     /locus_tag="KLK57_002329"
                     /pseudo
     CDS             9961..11189
                     /gene="sorE"
                     /locus_tag="KLK57_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312968.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
     gene            11572..12261
                     /gene="pepE"
                     /locus_tag="KLK57_002330"
     CDS             11572..12261
                     /gene="pepE"
                     /locus_tag="KLK57_002330"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HBE0892683.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TCDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(12352..13983)
                     /locus_tag="KLK57_002331"
     CDS             complement(12352..13983)
                     /locus_tag="KLK57_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HBE0892684.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(14203..17886)
                     /gene="metH"
                     /locus_tag="KLK57_002332"
     CDS             complement(14203..17886)
                     /gene="metH"
                     /locus_tag="KLK57_002332"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HBE0892685.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDGLV
                     VAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFETLGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAEAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWEVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNEPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            18086..18910
                     /gene="iclR"
                     /locus_tag="KLK57_002333"
     CDS             18086..18910
                     /gene="iclR"
                     /locus_tag="KLK57_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HBE0892686.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            19227..19598
                     /locus_tag="KLK57_002334"
                     /pseudo
     CDS             19227..19598
                     /locus_tag="KLK57_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4680 a         5094 c         5075 g         4749 t
ORIGIN      
        1 ccgttaaatc acagacaagg ggatttatct gataaaacaa tgccgactta ttgtcggcat
       61 tgtttttaag gttatttaac tcctcatact atgcattaat gtcgatattg gcatcaggct
      121 ccggttgccg gatgcggcgt aaacgcctta tccggcctac atatcggcat caggcacgca
      181 tgatgattaa ccgcgccacg ctttatagcg gttaatcaga ccattggtcg agctatcgtg
      241 gctgctgatt tctttgtcat ctttcagctc tggcagaata cggttcgcca gttgtttacc
      301 caactccacg ccccactggt cgaaggtaaa gatgttcagg atcacgccct gagtaaagat
      361 tttgtgctca tacagcgcaa tcaacgcacc caggctgaac ggagtgattt cgcgcagcag
      421 gatggagttg gtcgggcggt taccttcgaa cactttgaac ggcaccacgt agtcaagcgt
      481 tgccggatct ttaccctgat cgcgatattc ctgctcaacc acttcgcggg atttaccaaa
      541 cgccagcgct tcggtctggg cgaagaagtt agacagcagt ttctggtggt gatcagagag
      601 cgggttatgg gtgatagccg gggcgatgaa atcgcacggt accattttgg ttccctggtg
      661 gatcagctgg tagaacgcgt gctgaccgtt agtgcctggt tcaccccaga taatcgggcc
      721 agtctggtaa tccacaacgt taccgttacg gtcaacatac ttaccgttgg actccatatt
      781 gccctgctgg aagtacgccg cgaaacggtg catatactgg tcatacggca gaatcgcttc
      841 agtttccgca ccaaagaaat tattgtacca gatgccaatc agcgccagca ggacaggcag
      901 gtttttctcg gcaggcgtgg tggagaaatg cttgtccatc gcgtgtgcgc cagaaagcag
      961 ttcaacgaag ttatcaaagc cgatggagag aacaatcgac aggccaatcg ctgaccacaa
     1021 agagtaacgg ccgccgaccc agtcccagaa ctcgaacatg ttggcagtat caataccaaa
     1081 ctcgccaacg gctttggcat tggtggaaag cgccgcaaag tgttttgcaa cgtgtttttc
     1141 atcacctgcc gctttcagga accagtcacg cgcgctatgg gcgttggtca tagtttcctg
     1201 agtggtgaag gttttagatg ctaccaggaa cagcgtggtt tccgggttta cttttttcag
     1261 cacttcagcg atgtgagtcc catcgacgtt agaaacaaag tgcatgttca ggtgattttt
     1321 gtacggacgc agtgcttcgg tcaccatgta cgggccgagg tcagaaccac cgatcccgat
     1381 gttcactacg tcagtgattg ctttgccggt ataacccttc cactcaccgg agataatcgc
     1441 ttccgagaag gttttcatct tctccagcac cgcgttgact tccggcatta cgtctttgcc
     1501 atcaaccaga atcggggtat tgctacggtt acgcagcgct acgtgcagca cggcgcggtt
     1561 ttcagtgcgg ttgatcttct cgccagagaa catcgactta atcgcgcccg ccagatcgca
     1621 ctctttcgcc agatcctgta atttcgccag cgtctcttca gtgatgcggt ttttggagta
     1681 atccaccagc atctgatcgt cgaatgttgc ggagaactta gaaaaacgat cgccgtcttt
     1741 agcaaaaaga tcggcgatcg taacgtcttt catttcatcg aagtgtttct gtagtgcctg
     1801 ccaggcagcg gtctgcgttg gattgatgtt tttcattagc aatactcttc tgattttgag
     1861 aattgtgact ttggaagatt gtagcgccag tcacagaaaa atgtgatggt tttagtgccg
     1921 ttagcgtaat gttgagtgta aacccttagc gcagtgaagc atttattagc tgaactactg
     1981 accgccagga gtggatgaaa aatccgcatg accccgtcgt tgacaaccgc cccgctcacc
     2041 ctttatttat aaatgtacta cctgcgctag cgcaggccag aagaggcgcg ttgcccaagt
     2101 aacggtgttg gaggagccag tcctgtgata acacctgagg gggtgcatcg ccgaggtgat
     2161 tgaacggctg gccacgttca tcatcggcta caggggctga atcccctggg ttgtcaccag
     2221 aagcgttcgc agtcgggcgt ttcgcaagtg gtggagcact tctgggtgaa aatagtagcg
     2281 aagtatcgct ctgcgcccac ccgtcttccg ctcttccctt gtgccaaggc tgaaaatgga
     2341 tcccctgaca cgaggtagtt atgtctgaaa ttgttgtctc caaatttggc ggtaccagcg
     2401 tagctgattt tgacgccatg aaccgcagcg ctgatattgt gctttctgat gccaacgtgc
     2461 gtttagtagt cctctcggct tctgctggta tcactaatct gctggtcgct ttagcggaag
     2521 ggctggaacc tggcgagcga ttcgaaaaac tcgacgctat tcgcaacatc cagtttgcca
     2581 ttctggaacg tctgcgttac ccgaacgtta tccgtgaaga gattgaacgt ctgctggaga
     2641 acattactgt tctggcagaa gcggcggcgc tggcaacgtc tccggcgctg acagatgagc
     2701 tggtcagcca cggcgagctg atgtcgaccc tgctgtttgt cgaaatcctg cgcgaacgcg
     2761 atgttcaggc acagtggttt gatgtacgta aagtgatgcg taccaacgac cgatttggtc
     2821 gtgcagagcc agatgtagcc gcgctggcgg aactggccgc gctgcagctg ctcccacgcc
     2881 tcaatgaagg cttagtgatc acccagggat ttatcggtag cgaaaataaa ggtcgtacaa
     2941 cgacgcttgg ccgtggaggc agcgattata cggcagcctt gctggcggag gctttacacg
     3001 catctcgtgt tgatatctgg accgacgtcc cgggcatcta caccaccgat ccacgcgtag
     3061 tttccgcagc aaaacgcatt gatgaaatcg cgtttgccga agcggcagag atggcaactt
     3121 ttggtgcaaa agtactgcat ccggcaacgt tgctacccgc agtacgcagc gatatcccgg
     3181 tctttgtcgg ctccagcaaa gacccacgcg caggtggtac gctggtgtgc aataaaactg
     3241 aaaatccgcc gctgttccgc gcgctggcgc ttcgtcgcaa tcagactctg ctcactttgc
     3301 acagcctgaa tatgctgcat tctcgcggtt tcctcgcgga agttttcggc atcctcgcgc
     3361 ggcataatat ttcggtagac ttaatcacca cgtcagaagt gagcgtggca ttaacccttg
     3421 ataccaccgg ttcaacctcc actggcgata cgttgctgac gcaatctctg ctgatggagc
     3481 tttccgcact gtgccgggtg gaagtagaag aaggtctggc gctggtcgcg ttgattggca
     3541 atgacctgtc aaaagcctgc ggcgttggca aagaggtatt cggcgtactg gaaccgttca
     3601 acattcgcat gatttgttac ggcgcatcca gccataacct gtgcttcctg gtgcccggcg
     3661 aagatgccga gcaggtggtg caaaaactgc atagtaattt gtttgagtaa atactgtatg
     3721 gcctggaagc tatatttcgg gccgtattga ttttcttgtc actatgctta tcaataaacg
     3781 agcctgtact ctgttaacca acgtctttat cgagaataat tgcctttaat ttttttatct
     3841 gcatctctaa ttaattatcg aaagagataa atagttaaga gaaggcaaaa tgaatattat
     3901 cagttctgct cgcaaacgaa ttcccgtaca ctgaactctc tggctcactg agggcaccat
     3961 catggcactc ccccgtatta cccaaaaaga gatgactgaa cgcgagcagc gtgaacttaa
     4021 aacactgctg gatcgggcgc ggattgcgca tggtcgcgta ctgaccaact ccgaaaccaa
     4081 cagcattaag aaagagtaca tcgataaatt aatggtcgag cgtgaggctg aagcgaaaaa
     4141 agcccgccaa ttgaagaaaa agcaggctta taaaccggat cctgaagcgt catttagctg
     4201 gtcagcaaat acgtcgacgc gcggcaggcg ctaatgaaca atcaacgcct gagattttca
     4261 ctcgtctgtg tgaacacaat tttcccctca ccctaaccct tttcccgaag gggcgagggg
     4321 actgtccgag cacttttggg ccgttttcat cagtctgagg cgttaatcag cgccccttct
     4381 ttttacgccc cggcgaggta aaacgcttac cgttggacgc cgggcgaccg cctttttccg
     4441 ccgttttttc catcttaacg actggacgtt tgatgcccgc tgttttcggt ttggccttcg
     4501 ctttgggctt cgcctcggaa gaggaatttt caatgagctt aaagagatcg ataagctcgt
     4561 cgtcggttaa atcgcgccat tctcccagcg gaatgccgct taagctgacg ttcataatgc
     4621 gcgtacgttc cagctttttc acttcatagc cgaaatgctc gcacatgcgc cggatctgac
     4681 ggttcaaccc ctgcaccagg gtaatgcgga agacaaacgg tgcttctttt ttgactttgc
     4741 actttttcgt caccgtcccg aggatcggca ctcccgcccc cataccacga ataaattcat
     4801 cggtaatcgg tttatcgacc gtcaccagat actctttctc atgatcgtta cccgcacgga
     4861 ggatcttatt caccagatcg ccgtgattgg tgaggaaaat cagcccctgg gagtctttat
     4921 ccaggcggcc aatcgggaac acgcgtttgc tgtggttaac gaaatcgaca atgttatcgc
     4981 gctcgccatc ttcggtggtg ctgacaatac cgacgggctt gttcagggcg ataagtacca
     5041 aatcttcggc ttcccgaggc tcaatcaact gaccgtttac tttcacaatg tcgccgggtt
     5101 tcacctgatc accaatggtg gctcgcttgc cattaaggaa cacattgcct tgctcgatat
     5161 agcgatccgc ttcgcggcgt gagcaaattc cgctttcgct gatgtattta tttaaacgga
     5221 ctgatgagtc gggcagcata aattctcctg taaaacgcgg aatataccgt acctcttagc
     5281 ggggaaaaac agggttatgc ggcaacgtcg aaattaaatg tgatctgcca cgccttttat
     5341 cacaaaagtg tctccggaag tacgatgcaa atttgcctgg caaaataagg ataatcaaca
     5401 tattacgaat cgctgcacac ctgtgctgat gactactacg cagagaaaca aatgtgcaag
     5461 agaaagcgct atttctcccc atgcaaagag tgaaaaatcg ctttgaaagg ccctcataat
     5521 ggggttatat cagatcaagc ggtgagaaag cgtatttgca caaatgtgca gaaccaggag
     5581 gcggcaatgg aaaacagtga cgatatccgt ttgattgtga agattgccca actctattac
     5641 gaacaggata tgacgcaggc gcaaatcgcg cgcgaactgg gtatttaccg caccaccatc
     5701 agccgcttgc ttaaacgagg ccgcgatcag ggaattgtca ccatcgccat caactatgac
     5761 tacaacgaaa atctctggct ggagcagcaa ctgaagcaaa agtttggctt gaaagacgtt
     5821 gtggtggtgt cgggaaatga tgaggatgaa gagactcaac tggcgatgat ggggttacac
     5881 ggcgcgcaac tgctggatcg cttgctggag cctggcgata ttgtcggttt ttcctagggt
     5941 cgcgcggtga gcgcactggt tgaaaacttg ccgcaagcgg ggcaatcgcg gcagttaatc
     6001 tgcgtgccaa ttattggcgg accgtccggt aaactcgaaa gccgctatca cgtaaacaca
     6061 ttaacctaca gcgtggcagc gaagctgaaa ggggaatcgc atctcgcgga ttttccggct
     6121 ctgctggata accccttaat tcgtaatggg atcatgcagt ctcagcactt taaaaccatc
     6181 tctgcctact gggataatct ggatgtcgcc ctggtgggaa ttggctcacc ggccattcgc
     6241 gacggcgcta actggcatgc gttttatggt ggtgaagaga gtgacgacct gaaagcaggc
     6301 gcggtattcc attggcattg ccatgagtga agaaaaatac agcggaattg ttggtgcact
     6361 gcgtggaaaa tatattaatt gtctggtaac gaatagcagc acagctgaac tattactgaa
     6421 ataaaaatct tcccgatgtt taccgtaata aaaattacgg cgggtcccta ttatctaaaa
     6481 tcaggagtct gttatgcaaa cgtggttaaa tttgcaggat aaaatcatta tcgtcaccgg
     6541 cggcgcatcc ggtattggtc tggcgattgt agaggaatta ttagcacaag gcgcaaacgt
     6601 acagatggta gatattcacg gtggcgatgg tcaatatgaa gttcataaag gctatcagtt
     6661 ttggccgacc gatatttcca gcgccaaaga ggtaaatcat acggtagcgg aaattatcca
     6721 gcgttttggt cgcatcgacg gtctggtcaa taacgccggg gtcaatttcc cgcgtctgct
     6781 ggtcgatgag aaagcgcctg ccgggcagta tgaactcaac gaagctgcat tcgaaaaaat
     6841 ggtcaatatc aaccaaaaag gcgtttttct gatgtcgcag gcggtggcgc gccagatggt
     6901 caaacaacat gatggcgtga ttgtgaatgt ttcctcagaa agtggactgg aaggctcaga
     6961 aggccaaagc tgttacgccg ctaccaaagc cgcgctcaat agcttcacgc gctcctggtc
     7021 gaaagagctg ggtaaacacg gtatccgtgt ggtcggtatc gcgccgggga ttctggaaaa
     7081 aaccgggcta cgcacgccgg aatatgaaga ggcgctggcg tggacgcgca atatcaccgt
     7141 cgagcagctg cgtgaaggct ataccaaaaa cgccattcct attgggcgcg ccggaagatt
     7201 agcagaagtc gctgattttg tttgttatct gctgtctgaa cgcgccagct acatcaccgg
     7261 agtaaccact aacattgcgg gcggcaaaac gcgcggataa ggaggcttta tggtcaacgc
     7321 cattttttgt gcccacggca aattagcttg cgccatgctg gaatcggttc agatggtgta
     7381 cggcgacgcc aatgtcgagg cggttgaatt tgtaccaggc gagaacgccg gagacatcgt
     7441 agcaaagctg gaaaagttag taagcattca caatcaagat gagtggctga ttgccgtgga
     7501 tttacagtgc ggcagcccgt ggaatgcggc agcgatgctt gcgatgcgca atcctcgtct
     7561 acgggtaatc agcggccttt ccctgccgct ggcgctcgaa ctggttgata accaggacag
     7621 catgaatgtt gatgaactgt gtgaacacct gacgcaaatc gcaaagcaaa cctgcgttgt
     7681 ctggaaacaa ctggcaacgg cagaggaaga tttctgatga acattacgct cgcccgcatt
     7741 gatgaccgtc tgatccacgg ccaggtcact accgtctggt cgaaagtggc taacgcccag
     7801 cgcattatta tctgcaatga cgaagtttat aacgatgaag tgcgccggac attattgcgt
     7861 caggctgcgc caccgggaat gaaggttaac gtggtcaata ttgaaaaagc cgtcgcggtt
     7921 tatcacaacc cgcaatatta ggacgaaacc gttttttatc ttttcactcg cccacaggat
     7981 gccttagcga tggttcgcca gggggtaaaa ataggcaccc tgaatattgg cggcatggcc
     8041 tggcggcccg gtaaaaaaca gttaaccaaa gcggtttcat tagatgatga tgatattaac
     8101 gcatttcatg agttaaataa tcttggcgtc ataatggatt tacgcgtcgt agcgtcagat
     8161 ccctcaatca atattatcga caaaataaac gaacagttaa tcgcaaatta aataatagcg
     8221 cctgtgtaca tatgctttaa caggcaggga tgacgacact taaaaggtga caaatcatgg
     8281 aaataagtac cctacaaatt attgctatat ttcttttttc ctgtattgcc ggaatgggca
     8341 gcgtgctgga tgaatttcag actcatcgcc cgttaattgc ctgtacggtg attggtttaa
     8401 ttctcggtga tttaaaaacc ggaataatac tcggtggtac gctggaattg atagctctcg
     8461 gctggatgaa cgtcggcgcg gcgcaatctc cggattctgc actcgccagc ataatctccg
     8521 ccattctggt tatcgttggt cagcaaagca tcgccaccgg aatcgccatc gcgttgcctg
     8581 tggctgcggc aggccaagtg ctgacggtgt ttgcccgtac catcaccgta gtgttccagc
     8641 acgcggcgga taaagcagcg gaagaggcgc ggtttcgcac tctcgatatt ctgcatgtct
     8701 ccgcgcttgg cgtgcaggcg ctgcgcgttg ctattccggc actgattgtc tcactgttcg
     8761 tcagcgccga tatggtcagc aatatgttaa acgccattcc cgaatttgtg acccgtggac
     8821 tgcaaattgc tggcggtttt atcgtggtgg tcggttacgc catggtgcta cgtatgatgg
     8881 gcgtgaaata tttgatgcct ttctttttcc tcggtttcct cgcaggtggc tacctcgatc
     8941 tcagcctgct ggcgttcggt ggcgtcggcg tgatcatggc cctggtctac atccagctaa
     9001 atccacagtg gcgtaaagct gaacctcaac tccaggccac cgcctccacc gcccttgacc
     9061 aacttgacga ttaacggagc catcatggaa cagagaaaaa ttacacgcag cgatctggtg
     9121 agcatgtttc tgcgctccaa cctgcaacag gcgtccttta actttgaacg tattcacgga
     9181 ctgggctttt gctacgacat gatccccgcc atcaagcgac tttacccatt aaaagaggat
     9241 caggttgcgg cgctcaggcg acacctggtg ttcttcaata ccacgccagc cgtatgtggc
     9301 ccggtcatcg gcgtcactgc cgccatggaa gaggcgcggg ccaacggcgc ggaaattgat
     9361 gacggtacca ttaacggcat caaagtcggt ctgatgggac cattggcagg agttggcgat
     9421 ccactggtct ggggaacgct gcgcccgatt accgctgcgc tcggcgcatc tctggcactt
     9481 tctggcaaca tcctcggccc gctgctgttt ttctttattt tcaatgcggt gcgtctggcg
     9541 atgaagtggt atggcctaca gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg
     9601 ggcgggaatg tgctgcaaaa actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg
     9661 ggcgtgctgg tcaccaaatg gacgtcaatc aacgtaccgt tggtggtttc acaaacgcat
     9721 gccgccgatg gctccaccgt catcatgacc gtgcagaaca ttctcgacca actttgccct
     9781 ggtttgctgg cgctcggtct gacgctactg atggttcgtc tgctcaacaa aaaaattaac
     9841 ccggtatggc tgattttcgc cctgtttggc ttagggatta tcggcaatgc gctgggcttc
     9901 ctgtcctgat tcttcgcccc ggcacgactg ccggggccat cgctcaacat gaggtggttt
     9961 atgaaaacaa cagctctgcg tctttatggt aaacgtgatt tacgcctgga aacctttgac
    10021 cttcctgaaa tgcaggagga tgaaatcctc gcgacggtgg tcactgacag cctgtgcctc
    10081 tcttcctgga aagaggcaac ctgggtgaaa accataaaaa agtacccgac gatgtggcga
    10141 ccaacccaat catcatcggc cacgagtttt gcggcgatat tctggccgtg ggtaaaaagt
    10201 ggcagcacaa attccagccg ggtcagcgtt atgtgattca ggccaacctg aaactccctg
    10261 accgcccgga ctgccccggc tactccttcc cgtgggtggg cggcgaagcc acgcatgtgg
    10321 ttattcccaa cgaggtcatg gaacaagatt gcctgctggc atacgacggc gaaacctatt
    10381 ttgaaggctc gctggttgaa ccgctttcct gcgtgattgg cgcgttcaac gccaactatc
    10441 atcttcagga aggtagttat aaccacacga tggggattcg cccgcaaggg cgcacgctga
    10501 tcctcggcgg caccggacca atgggattgt tggcgattga ttatgcgctg catggaccag
    10561 ttaacccgtc gctgctcgtc attaccgata ccgacaacga caaattgagt tatgcgcgca
    10621 agcactatcc atcagaaccg caaacactga ttcattatct caatgccacc gatgcagcat
    10681 ttgatacgtt aatggcgctg agtggcggtc acggcttcga tgatattttc gtctttgtgc
    10741 ctaatgaagg actggtgact ctcgcctctt ccttgctggc gacagatggt tgcctgaatt
    10801 tcttcgccgg accgcaggat aaacatttca gcgcgccaat taatttctac gatgtgcatt
    10861 atgcatttac ccactacgtg ggcacgtcag gcggcaatac cgacgacatg cgcgcggcgg
    10921 tcaaactgat tgaagagaaa aaagtgcagg ccgcaaaagt ggtaacacat attcttgggc
    10981 tgaatgccgc gggcgaaacc acgcttgaat tgcctgccgt cggcggcggc aaaaagctgg
    11041 tgtataccgg gaaatacctg ccgctgacgt cactcacgca gattcaggat caagcactgg
    11101 cggcgattct ggcgcgtcat caggggatct ggtccggtga ggcggagcaa tatctgctca
    11161 ctcatgcaga ggcaatttcc catgattaat cgcgctactt aactctgcat gtcgctggct
    11221 ggtcgccgcg gcaacttcgc gacctgatgt gcggctgatt gctgaagcgg cccaatgtgc
    11281 acgagaagga taaaaaaaca tcagcgcctt aaggggcgct caaattggtg gcaaagtaca
    11341 cattgtttat gccggatgcg gaataaagcc ttattcggcc tacataatct cgaaaattca
    11401 atatattgca gttatctggt aggcctggta agcgtagcgc atcaggcaat gctgtgtttg
    11461 tcatcagtct cagcgcccgg tgcaatacgt gacatcgtga gccatgtcag taagtggtta
    11521 aattcgcagt ggtagcatgc cttccgtgat ttcttactaa ccggagaagt aatggaactg
    11581 cttttattga gtaactcgac gctgccgggt aaagcctggc tggaacatgc actgccgcta
    11641 attgctgaac agttgcaggg tcgccgctca gcggtgttta tccctttcgc tggcgtaacg
    11701 cagacctgtg atgattacac agcgaaaacg gctgcggttc tcgctccgct gggtgtttct
    11761 gtcaccggta ttcatagcgt tgtcgatccc gttgccgcga ttgaaaatgc tgagatcgtg
    11821 attgtcgggg gcgggaatac tttccagttg ctgaaacagt gccgcgagcg cgggctgctg
    11881 gcaccaatta ctgacgtggt taaacgtggt gcgctgtata ttggctggag cgcaggcgct
    11941 aaccttgctt gcccaaccat tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt
    12001 ttcgatgcgc taaatctgtt cccgctgcaa atcaacccgc acttcaccaa cgcgctgccg
    12061 gaaggccata aaggtgaaac ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca
    12121 gaactgacga ttattggtct accggaaggt aactggatca cggtgagtaa aggtcacgct
    12181 acgctgggtg gcccgaacac cacttatgtg tttaaggccg gtgaagaagc ggtgccgctg
    12241 gaagcgggtc accgttttta agtgaattgc cggatgcatg atccggcatc ttgttcatag
    12301 tgcctgatgc gacgcttgac gcgtcttatc aggcctacag gattcacaat attaatactc
    12361 gtcccgtcct tcatcttcat ccggctgttc cagcacactg taagccaccg aacaaaacag
    12421 cgagttcaga cgctgcatat ctcccagtaa gcctaaatgt agcgaactgg tttcaatgct
    12481 ctgcacgttt tgctgatgca ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat
    12541 gcgaaagcga tgcttgctgc ggcgcaaacg acgagcgctg gtgacatcgc cagagaagaa
    12601 caccgacatt gccagcttta aattactgag caattgctca tagagcgcat ccagttcctt
    12661 caacccatca agcgaaaatg cccgccgtgc tgccagcgat ttatcagcaa tttcgctgcc
    12721 catgcgctcg acgatatcgg aagcctgttc aaggttgagc gacatttcaa taatctccgc
    12781 ccagcgacgc gactcttctt ctgccagttc ctcttttggc attcgcgcca gatacagctt
    12841 aatggcggtg tagagaacgt tgatatcatc tgccagctta cgcagctctt tctcctgccg
    12901 tggctcgccg tgcatcactt tattcagccc ttccatcatc tgttccatgg cgtcgccaat
    12961 gcgcagggtt tcgcgcgcgg cgttcgccag agcaagcgtg ggcgtatcca gcgcgctgac
    13021 atccagatgt ttaggccgta gctgggtatc cagttccggt tcatcgcgaa taatcgtttt
    13081 gcaaaaccgt gccatcgggt caacaaatgg cagcatgacc aggcaacgta caaggttgta
    13141 gaagacgtgg aaatagatca ccagttccgc ttttggcaat gacaacttcc ccattgtctc
    13201 tgccagcaaa tggacaaacg gcaggataat caggctaccc accagcttaa acagcaaact
    13261 acccagcgcg acacggcggg ctgcggcatt ggcggcactg ttgttgagca tcgccagcag
    13321 gccggaaccg aggttagcac caatcaccag acagagcgcc acggggaagg agataatgcc
    13381 tgcggcggtc agagtcgcgg tcagcagtac agcagcaagg ctggagtagc tgataatggc
    13441 gaacatcgcg ccaatcagcg catccagcag aatatcgccg gtcagcgagg caaagatcac
    13501 ctgaacgccg tttgcctggg tgatcggcgt tacggcctgc acaatcaact ccagcgccag
    13561 caaaatcagc ccaagaccaa taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg
    13621 tccgaggaaa aaaatcacgc cgataaaaat aagtaacggt gacagccagg ataagtcgaa
    13681 ggtgagaata cgcgccatta gcgccgtccc gacatctgca cccagcacaa tcaccagagc
    13741 cggtgcgagg gctaccagat cctgagcgac gaacgaggtc accagcatgg tggtggcatt
    13801 actgctctgt accagtgcgg taacgccgat ccccgcgcaa aaggcgagcg gctttttttc
    13861 gacgctccgg ctaaggacag tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg
    13921 aacaatatga gtcccccaga ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa
    13981 cacgagttca ggttctcctt atcgttatat gttcctgaag caattgaatg accaaaggac
    14041 cggaaacacg ctgactggca tctttactgt gccttttgac attcgttttt ccggctaaga
    14101 ttattccagc ctgtttccag tataagtgtt tcatattaat aaagagacag ggcactcatt
    14161 gagcgccctg tttgttgtaa ggaaaagtga cagatttgtg aatcagtccg cgtcataccc
    14221 cagattcggt gccagccagc gctcaacatc ggagacgctc atacctttac ggcgggcata
    14281 gtcttcaacc tgatcgcgct gaatttgcgc caccgcgtag tacttgctgt ccgggtggct
    14341 gaaataccaa cccgaaaccg atgcaccagg ccacatggcg aaagattctg tgagtttcat
    14401 gccagtatgt ttttccactt ccagcagctc ccagatggtg gctttttccg tatgttccgg
    14461 gcaggccggg tagcccggtg ccggacggat gccctggtag ttttcgcgga tcagttcttc
    14521 gttgctgagg ttctcgttcg gcgcatagcc ccagtagact ttacgcacac gctcatggag
    14581 atactccgca aaggcttcgg ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta
    14641 atcatcgtgc tgcgcttcaa aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac
    14701 ggcaaatgcg ccgatgtaat ctgctttacc agaaagcttc ggcgcaacga agtcagcgag
    14761 acagtagtta gcgaatcccg ttttttcggt ctgttgacgc agatggtggc tgacgttgat
    14821 cacatgggtg cgcgtttcgt cacggtagat ttcaatgtca tcgcccacac ggtttgccgg
    14881 gaacaagccc accacgccac gcggattcag cgttttctcg gcgcttaatt tatccagcat
    14941 gtcgttggcg tctttaaaca gccgctgcgc ctcaacgccc accacttcat cttccagaat
    15001 gcgcggatac ttcccggcca gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt
    15061 acgcagagtt tcgatgctgg cttcaacttc ctgcacgccg agacggtgcg ccaccggcgg
    15121 cgtgtaagcc tgccagtcaa aagcgaagtc gttatcgcgc gccgcttcca gcgtgaccgg
    15181 tggtgtgcgc ggtttcttgc gcccgtgctg aatacgtacg gtttcatact ccttgcgggt
    15241 acgagcgaca aaatcatcgc gctgagtatc ggaaagcagc gccgccacca caccaacggt
    15301 gcgcgaggca ttctgcacat acaccgtcgg gccgctgtag ttctgctcaa ttttcaccgc
    15361 cgtgtgcgct tttgaggtcg tcgcgccacc gatcagtaac ggaatggtga agccctgacg
    15421 ctccatctct ttcgccacgt taaccatctc gtccagcgac ggcgtgataa gccccgaaag
    15481 tccaatcaga tcagcattca cttctttagc ggtacggaga attttttccg caggcaccat
    15541 aacgccgaga tcgacaattt cgtagttgtt acattgcagc accacaccaa cgatattttt
    15601 accgatgtcg tggacgtcgc ccttcacggt ggcgatcacc atcttgccgt tggttttgcc
    15661 ctgctctttg ctggcttcaa taaacggttc gaggtaggcc actgcctgtt tcatgacgcg
    15721 cgccgatttg accacctgtg gcaggaacat tttcccctcg ccaaacagat cgccgaccac
    15781 gttcatgccg tccatcaacg ggccttcaat cacttcaatc gggcgcgtag cctgctggcg
    15841 ggcttcttcg gtatcctgct cgataaactc agtaatgcct ttgaccagcg agtattccag
    15901 acgtttattc acttcccacg agcgccactc cgcctgctgg gcgttagcgg tgtcgtcggt
    15961 tttgctgccg cgatatttct cggcaagctc cagtaaacgt tcagtgccat catcgcgacg
    16021 attaagaatc acatcttcca ccgcgtcgcg cagttcagcg ggcaggtcgt cgtaaatcgc
    16081 cagttgcccg gcgttgacga tccccatatc catgccattg cgaatagcgt agtagaggaa
    16141 caccgcgtga atggcttcgc gcaccggttc gttgccacgg aatgagaaag aaacgttaga
    16201 tacgccgccg gaaatcagcg cgtgcggcag ttcgcgtttg atgtcttcgc aggcgccgat
    16261 aaagtcctgc gcatagttgt tgtgctcttc aatgccggtg gcgaccgcga agatgttcgg
    16321 gtcgaagatg atatcttctg gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg
    16381 acggcaaatc tcgattttcc gtgcgcgggt atcggcctgt ccctgttcgt caaaggccat
    16441 taccaccacc gctgcaccgt aacgacgcaa cagtttcgca tgatggataa aggcgtcgac
    16501 gccctctttc attgagatag agttaacaat gcctttgccc tgaatacact tcagcccctt
    16561 ttcgatgacc tcccattttg aggagtcgat cataatcggc acgcgagcga tatccggttc
    16621 accggcaatc agattgagaa aacgcaccat cgccgcttcg gcgtcgagca tcccttcatc
    16681 catattgata tcgataatct gcgcgccgtt ttccacctgc tggcgggcca catccagtgc
    16741 ctcgctgtat ttctcttctt tgatcaggcg cttgaactta gcggaaccgg tgacgttggt
    16801 gcgttcaccc acgttcacaa acaggctatc ttcgccaatg ttcagcggct ccaggccgga
    16861 caaacggcag gctacgggga tttccggcag tttgcgcggc gctaatcctt ctactgcacg
    16921 actcatcgct gcaatatgtt gtggcgtggt gccacagcag ccgccgacga tattgagaaa
    16981 gcccgcttcc gcccattcac gtatttgttt tgccatcgtg tcggcgtcga gatcgtactc
    17041 accaaaggcg ttgggtagcc cggcgttcgg gtgcgcggtg acgtagcatt ccgcaatccg
    17101 tgacagctcc tgcacgtact ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa
    17161 ggtcagcgct tcggcgtggc gcaatgagtt gtaaaatgct tcggtggtct gcccggagag
    17221 cgtgcgcccg gaggcgtcgg tgatggtgcc agagatcata atcggcagct caacgcccag
    17281 cgtttcaaac tccgttttta ccgcaaatac cgccgctttg gcgttaaggg tgtcgaaaac
    17341 ggtttcaatc aggatcagat ccgcaccacc ttccaccagc gctttggtgg actctcgata
    17401 agccaccacc agcccgtcaa aagtgatatt acgaaatgcc ggatcgttga cgtccggaga
    17461 aatagacgcc gtgcggttgg ttgggccgag aacaccggca acgtagcgcg gtttctccgg
    17521 cgtgcgcgcg gtccactcgt cagcacaggc gcgcgccagt ttcgccgccg caaagttgat
    17581 ttccgccgac agggattcca tctggtaatc cgccatcgca atggtcgtgg agttgaaggt
    17641 gttggtttcg atgatatccg cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac
    17701 ttccggttta ctgagtacca gcaggtcgtt gttgcctttg aggtcgcatg gccagtcggc
    17761 aaagcgttca ccacgaaaat cggcttcgtt cagtcgatag ctctggatca tggtgcccat
    17821 accgccgtcc agcaccagaa tacgttcatt taactgcgca cgcagttgtt ccactttgct
    17881 gctcacactt gctcccgaca cgctcaaccc agatttaata aaaattcaac aaaccatact
    17941 ggcataaacg catctgtggt aaaagcgacc accacgcaac atgagatttg ttcaacatta
    18001 actcatcgga tcagttcagc aactattgca ttaactaaca ataaaaatga aaatgatttc
    18061 cacgatacag aaaaaggaga ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa
    18121 acccgccgtt gccaccgcac cagcgactgg acaggttcag tctttaacgc gtggcctgaa
    18181 attactggag tggattgccg aatccaatgg cagtgtggca ctcacggaac tggcgcaaca
    18241 agccgggtta cccaattcca cgacccaccg cctgctaacc acgatgcaac agcagggttt
    18301 cgtgcgtcag gttggcgaac tgggacactg ggcaatcggc gcacatgcct ttatggtcgg
    18361 cagcagcttt ctccagagcc gtaatttgtt agcgattgtt caccctatcc tgcgcaatct
    18421 aatggaagag tctggcgaaa cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc
    18481 gattattatc gatcaggtac agtgtactca cctgatgcgt atgtccgcac ctatcggcgg
    18541 taaattgccg atgcacgctt ccggtgcagg taaagccttt ttagcccaac tgagcgaaga
    18601 acaggtgacg aagctgctgc accgcaaagg gttacatgcc tatacccacg caacgctggt
    18661 gtctcctgtg catttaaaag aagatctcgc ccaaacgcgc aaacggggct attcatttga
    18721 cgatgaggaa catgcactgg ggctacgttg ccttgcagcg tgtattttcg atgagcaccg
    18781 tgaaccgttt gccgcaattt ctatttccgg accgatttca cgtattaccg atgaccgcgt
    18841 gaccgagttt ggcgcgatgg tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg
    18901 aatgcgctga ctttttctgg cgggcagagg caatattctg cccatcatac ctgagtggca
    18961 atagaataag ggtgtctgtt aatcgcattg acgccaaaat aacttaatgt catacacttc
    19021 acacactttt attaactcag cattattttt aaacatcaaa ccacttaatt attaacaaaa
    19081 tcgcaagatc tcattccacc gaaactttta cggctgcatt atgagattga gcgatttatg
    19141 atatttcttt agttgtaatc atattaaatt ttttcactgt tctgatagtt aaaatttaag
    19201 acatcaataa acaatgagat atttaaatga ttactcgtat tcctcgtagt tctttctctg
    19261 caaatattaa taatacagcc cagacaaatg aacaccaaac cctgagtgaa ttgttttata
    19321 aagaactcga ggataaattt tctggcaagg agctggcgac gcctctatta aaaagcttct
    19381 cagagaactg tagacaaaat ggtcgtcata tctttagcaa caaggatttt gtcattaaat
    19441 tttccacgtc cgtcttacaa gctgataaga aagaaattac gataattaat aaaacgaaaa
    19501 cacgacactc actcaaacca ttgctccaat atttgaagaa tacctaatgg aaattttacc
    19561 tcaacgctca gacactcttg ataaacaaga attaaacc
//
LOCUS       DAEEKQ010000070        19408 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.070, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000070 DAEEKQ010000000
VERSION     DAEEKQ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19408
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.070"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..245
                     /locus_tag="KLK57_002335"
     CDS             <1..245
                     /locus_tag="KLK57_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HBE0892687.1"
                     /translation="AHADYPGDEYQFGKEIAESELLWPVDRVQKSLNGELQQINGADY
                     FIFGHMMFDNIQTFANQIYIDTGTPKSGRLSFYKIK"
     gene            complement(296..1063)
                     /locus_tag="KLK57_002336"
     CDS             complement(296..1063)
                     /locus_tag="KLK57_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE0892688.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            1259..2167
                     /locus_tag="KLK57_002337"
     CDS             1259..2167
                     /locus_tag="KLK57_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBE0892689.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVANAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            2164..3426
                     /locus_tag="KLK57_002338"
     CDS             2164..3426
                     /locus_tag="KLK57_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HBE0892690.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALSVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGRPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            3423..4061
                     /locus_tag="KLK57_002339"
     CDS             3423..4061
                     /locus_tag="KLK57_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBE0892691.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            4066..4842
                     /locus_tag="KLK57_002340"
     CDS             4066..4842
                     /locus_tag="KLK57_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HBE0892692.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGYEYKPRGLTEEGLGWFDAWR"
     gene            4931..6295
                     /locus_tag="KLK57_002341"
     CDS             4931..6295
                     /locus_tag="KLK57_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HBE0892693.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINKHQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="KLK57_002342"
     CDS             complement(6389..7381)
                     /gene="rpoS"
                     /locus_tag="KLK57_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HBE0892694.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="KLK57_002343"
     CDS             complement(7444..8583)
                     /gene="nlpD"
                     /locus_tag="KLK57_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HBE0892695.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="KLK57_002344"
     CDS             complement(8723..9349)
                     /gene="pcm"
                     /locus_tag="KLK57_002344"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HBE0892696.1"
                     /translation="MVSRRVQALLEQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(9343..10104)
                     /gene="surE"
                     /locus_tag="KLK57_002345"
     CDS             complement(9343..10104)
                     /gene="surE"
                     /locus_tag="KLK57_002345"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HBE0892697.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(10085..11134)
                     /gene="truD"
                     /locus_tag="KLK57_002346"
     CDS             complement(10085..11134)
                     /gene="truD"
                     /locus_tag="KLK57_002346"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HBE0892698.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGAQRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="KLK57_002347"
     CDS             complement(11131..11610)
                     /gene="ispF"
                     /locus_tag="KLK57_002347"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HBE0892699.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="KLK57_002348"
     CDS             complement(11610..12320)
                     /gene="ispD"
                     /locus_tag="KLK57_002348"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HBE0892700.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="KLK57_002349"
     CDS             complement(12339..12650)
                     /gene="ftsB"
                     /locus_tag="KLK57_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HBE0892701.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(12844..13167)
                     /locus_tag="KLK57_002350"
     CDS             complement(12844..13167)
                     /locus_tag="KLK57_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HBE0892702.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="KLK57_002351"
     CDS             complement(13217..13822)
                     /gene="cysC"
                     /locus_tag="KLK57_002351"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HBE0892703.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="KLK57_002352"
     CDS             complement(13822..15249)
                     /gene="cysN"
                     /locus_tag="KLK57_002352"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HBE0892704.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="KLK57_002353"
     CDS             complement(15251..16159)
                     /gene="cysD"
                     /locus_tag="KLK57_002353"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HBE0892705.1"
                     /translation="MDQIRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            16411..17448
                     /gene="iap"
                     /locus_tag="KLK57_002354"
     CDS             16411..17448
                     /gene="iap"
                     /locus_tag="KLK57_002354"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HBE0892706.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="KLK57_002355"
     CDS             complement(17716..18009)
                     /gene="cas2"
                     /locus_tag="KLK57_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HBE0892707.1"
                     /translation="MSMVVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQITQLAGCGNVVMAWATNIESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="KLK57_002356"
                     /pseudo
     CDS             complement(18006..18929)
                     /gene="cas1e"
                     /locus_tag="KLK57_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endonuclease Cas1"
     gene            complement(18926..>19408)
                     /gene="cas6e"
                     /locus_tag="KLK57_002357"
     CDS             complement(18926..>19408)
                     /gene="cas6e"
                     /locus_tag="KLK57_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBE0892708.1"
                     /translation="RFFVLSQERPAESDIFTIECRPFAPELRTGQSLCFNLRANPTIC
                     KAGKRHDLLMEAKRQVRGQVEGSDVWLHQQQAALDWLAAQGERSGFTLLDTSVDAYRQ
                     QQLRRENSRQLIQFSSVDYTGMLTVTAPGLFLQRLSQGYGKSRAFGCGLMLIKPGAEA
                     "
BASE COUNT         4592 a         5231 c         4848 g         4737 t
ORIGIN      
        1 tcgcacatgc agattatccg ggtgatgaat atcagtttgg taaagaaata gcagaaagcg
       61 aattactctg gcctgttgat cgtgtgcaga aatcgcttaa tggcgagtta caacaaataa
      121 acggcgctga ttattttata tttggacata tgatgtttga taacattcag acgttcgcta
      181 accagattta tattgatacc ggcacgccga aaagcgggcg gctgtcattt tataaaataa
      241 agtagtctca tgcttcttct gtgaagcatg agtaaccaaa tgttattgca ggccattatt
      301 catttttcgc taccagcaaa gagagatcct gcttcgccag cgcgcgactg gcactctccg
      361 gcaaaccgtc gtctgtgata atctgatcaa actcgcttaa tggtaacgcc agccatgtcg
      421 ccacctgacc atatttcgtc gcatcacaga ccaaaactcg ctggcggctg gcactggcaa
      481 tcgcccgttt caccgtgact ttatcttccg ctggcgtaga aatcccccgc acactccatg
      541 acgatgcaga aataaaagcc tgatcaatca tcaggctgcg cagcatggtc gcagcggctt
      601 ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc agtgtgaata attgtgcaat
      661 tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt tgtgaccaca gtcagtgact
      721 ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt tcccgcatcc agatagatac
      781 aacttcccgg ctgaacaaga cttgccgcca gcttgccaat agccgctttt tgcgtcattg
      841 ccagcgcagt ttttacctga tgagaaggtt catgcgccac gcgtcccgga gactggacgc
      901 ctccggacac cagcacaacg gctccctgct gctccagttt ttgtaaatcc cgacgaatgg
      961 tcatatgtga cacattcatt ctgtccgtta gttcagcaat actgacaatg cctttttcag
     1021 ctaccatctc aaggatgatt tggcgacgct ctacgggtat caacttttgc tccttccttt
     1081 gtcctgctga cattctacgc tatttgcctg cgaaacgtgc gcggcgcaac taacgcttag
     1141 ttcacataaa ataacacaca atgttaattt atgtgaatca gctcaccata tcgttatctt
     1201 ccagcgctta tattcacaat atcaaacaaa atatcactta aattaacaat gagagcagat
     1261 gaaaacggga tctgagtttc atgtcggtat cgttggctta gggtcaatgg gaatgggagc
     1321 agcactgtca tgtgtccgcg caggtctttc tacctggggc gcagacctga acagcaatgc
     1381 ctgcgctacg ttgaaagagg caggtgcttg cggggtttct gataacgccg cgacgtttgc
     1441 cgaaaaactg gacgctctgc tggtgctggt ggccaatgcg gcccaggtta aacaggtgct
     1501 gtttggtgaa acaggcgttg cacaacatct gaaacccggt acggcagtaa tggtttcttc
     1561 cactatcgct agtgctgatg cgcaagaaat tgctaccgct ctggctggat tcgatctgga
     1621 aatgctggat gcgccagttt ctggtggtgc agtaaaagcc gctaacggtg aaatgactgt
     1681 catggcctcc ggtagcgata ttgcctttga acgactggca cccgtgctgg aagccgttgc
     1741 cggaaaagtt tatcgcatag gtgcagaacc gggactaggt tcgaccgtaa aaattattca
     1801 ccagttgtta gcgggcgtac atattgctgc cggagccgaa gcgatggcac ttgcagcccg
     1861 tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc aatgccgccg gaaattcctg
     1921 gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat tacaccccgc attcagccgt
     1981 cgatattttt gttaaggatc ttggtctggt tgccgataca gccaaagccc tgcacttccc
     2041 gctgccattg gcctcaacag cattgaatat gttcaccagc gccagtaacg cgggttacgg
     2101 gaaagaagac gatagcgcag ttatcaagat tttctctggc atcactctac cgggagcgaa
     2161 atcatgatca agattggcgt tatcgccgat gattttaccg gcgcgacgga tatcgccagt
     2221 tttctggtgg aaaacggtct accaacggta caaattaacg gtgttccaac aggtaaaatg
     2281 ccggaagcaa tcgacgcact ggtgatcagc ctgaaaacgc gctcctgtcc ggtggttgaa
     2341 gccacacagc aatcgctggc ggctctgagc tggttgcaac agcaaggttg caaacagatc
     2401 tatttcaaat actgctctac tttcgacagt acggcgaaag gtaatattgg cccggttacc
     2461 gatgccttaa tggatgctct cgacacgccg tttacggtct tctctccggc cctgtcggtc
     2521 aacggacgta cggtttatca ggggtatttg ttcgtaatga atcaactgct ggccgaatcc
     2581 gggatgcgcc atcacccggt aaatcccatg accgacagct atcttccccg tctggttgaa
     2641 gcgcaatcca cagggcgctg cggcgtcgtt tcggcacatg ttttcgaaca aggcgtggat
     2701 gccgttcgtc aagagctggc tcgcttacag caagagggct accgctacgc ggtgcttgat
     2761 gcgctgaccg aacaccatct ggaaattcag ggagaagcct tgcgcgatgc cccactggta
     2821 acgggcggtt ctggtctggc gattggcctg gcccggcagt gggcgcaaga aaacggtaac
     2881 caggctcgcg aagcagggcg tccgctcgct gggcgcggcg tagtgctctc cggttcatgc
     2941 tctcaaatga ccaaccgcca ggtggcacat taccgtcaaa ttgcaccagc ccgtgaagtt
     3001 gatgtggcac gctgcctctc aaccgaaact ctggccgctt atgcacacga actggcagag
     3061 tgggttctgg gccaggaaag tgtacttgct ccactggttt ttgccaccgc cagcactgac
     3121 gcattggcag caattcaaca gcaatacggt gcacaaaaag ccagtcaggc agtagaaact
     3181 ctgttttctc aactagcggc gcggttagca gcggaaggcg tgacacgctt tattgtcgca
     3241 ggcggtgaga cctccggcgt agtcacacag agcctgggca taaaagggtt tcatattggc
     3301 ccaaccattt cccccggcgt gccgtgggta aacgcactgg ataagcctgt ctcactcgcc
     3361 cttaaatctg gcaacttcgg tgatgaagcc tttttttcac gagcccaaag agagttttta
     3421 tcatgagcga tttcgcaaaa gtagagcagt ctttgcgaga ggagatgacg cggattgcca
     3481 gttcattctt tcagcgcggc tatgcaaccg gttcggctgg caatctgtcg ctgcttttac
     3541 ctgacgggaa tttactggcg acaccgacag gttcatgcct gggcaatctc gatccgcagc
     3601 ggctttccaa agtcgccgcg gatggcgaat ggttaagtgg tgacaaaccc tcgaaagagg
     3661 tgctctttca tctggcgctg tatcgcaaca atccgcgctg taaagcggtg gtgcatttgc
     3721 acagcacatg gtcgacggcg ctttcctgcc tgcaagggct ggacagcagc aacgttattc
     3781 gtccgttcac accatacgtg gtgatgcgga tgggaaatgt cccgctggtg ccttattacc
     3841 gaccgggcga taaacgcatc gcacaggatc tggcggaact ggcagcagac aatcaggctt
     3901 ttttactggc aaatcatggc ccagtggttt gcggtgaaag cctgcaagaa gccgccaaca
     3961 atatggaaga gctggaggaa acggcaaagc tgatttttat tctcggtgac cgcccgatcc
     4021 gttatctgac cgcaggtgaa attgcggaat taaggagtta atgcaatgcc tcgttttgca
     4081 gctaatttat ccatgatgtt caccgaagtg ccttttattg aacgcttcgc cgcagcgcga
     4141 aaagccgggt tcgatgctgt ggaatttctg tttccctatg actactccac cctgcaaatc
     4201 caaaagcaac tggagcaaaa ccatctgaca ctggcgctgt tcaataccgc tcccggagat
     4261 attaatgcgg gggaatgggg attatccgcc cttcccggac gtgagcacga agcacacgca
     4321 gacatcgacc tggcactgga atatgcattg gcgctcaatt gtgaacaagt ccatgtgatg
     4381 gcaggcgtcg tgcccgctgg cgaagacgct gaacggtacc gggcagtatt tatcgataat
     4441 ctccgctacg ctgccgaccg ctttgcgcta cacggcaagc gaatattagt tgaagcactc
     4501 agccccgacg ttaagcctca ttatctcttc tccagccagt atcaggcact ggctattgtt
     4561 gaagaggttg cgcgagataa cgtgtttatt caactagata cttttcatgc gcaaaaagta
     4621 gatggcaacc tgacgcattt aatccgcgac tacgctggga aatatgcgca cgtacaaatt
     4681 gccggactac ctgatcgaca tgaaccggac gatggagaaa tcaactatcc gtggctgttc
     4741 cgcctgttcg atgaggtggg atatcagggc tggatcggtt atgaatataa acctcgtggc
     4801 ctcaccgaag aagggcttgg ctggtttgac gcctggcgct aattcgttaa atccctaatt
     4861 acaacgtacc catacatccc cccatctacg gatggggtag gttaactata actttcagac
     4921 agggtttccc atgtccacaa ttacattgtt atgcattgcg ttagctggcg taatcatgct
     4981 gttgctgctg gtcatcaagg caaaggtaca accattcgtt gctctgctcc tcgtcagcct
     5041 gttagtcgca cttgcggcgg gtataccggc gggcgaagtg ggtaaagtga tgatcgccgg
     5101 gatgggcggt gtgcttggtt ccgtcactat tattattggt ctgggcgcta tgctggggcg
     5161 tatgatcgaa cactctggtg gtgcagagtc actggctaat tatttcagtc gcaagttagg
     5221 tgacaaacga actatcgctg cgctgactct ggcagcgttc ttcctcggta ttcccgtctt
     5281 ctttgatgtc ggctttatta ttcttgctcc aatcatttac ggttttgcca aggttgccaa
     5341 aatatcgcca ctcaaatttg gcctgcctgt cgctgggatc atgctaactg tacatgtggc
     5401 ggtaccgccg catccaggcc ctgtcgccgc agcggggtta ctccacgcag acatcggctg
     5461 gctaaccatc atcggtattg cgatttctat tcccgtaggg attgttggct actttgcagc
     5521 gaaaataatc aataagcacc aatatgcgat gtcggtggaa gtactggaac agatgcaact
     5581 ggctccggcc agtgaggaag gcgcgacaaa attaagcgat aaaataaatc caccgggcgt
     5641 cgcgctggtc acctcgctaa ttgttattcc tatcgcgatt atcatggcgg gtacggtttc
     5701 cgcaacactg atgccgcctt cgcatcccct gcttggtacg ctacagctga tcggctcacc
     5761 aatggtagct ctaatgattg cgctggtgct ggcattctgg ttattggctt tacgtcgcgg
     5821 ctggagctta caacacacca gcgacattat gggctcagcc cttcccactg cggcggtagt
     5881 gattttggtt actggtgctg gaggggtatt tggcaaagtg ctggtggaat cgggcgtcgg
     5941 caaagccctt gccaacatgc tacaaatgat tgacctgcct ctgttaccag ccgcatttat
     6001 tatttcactg gcgctgcgtg catcgcaggg gtcagcaacc gtagcaatcc tgacaaccgg
     6061 cgggttactc tcagaagcgg tgatgggatt gaatccgatt cagtgcgtat tggtgacgct
     6121 ggcagcctgc tttggtgggc ttggggcgtc acatattaat gactcagggt tctggattgt
     6181 gaccaaatat ctggggttgt cggtagcaga cggtctgaaa acctggacag tgttaacgac
     6241 cattctcggt tttaccggat tcttaattac ctggtgcgta tgggcggtaa tttgacctta
     6301 ataaaaaggt ccgatgagca tcggaccttt tattgtgcac agaaaaggcc agcctcgctt
     6361 gagactggcc tttctgacag atgcttactt actcgcggaa cagcgcttcg atattcagcc
     6421 cctgcgtttg caggatttca cgcaaacggc gcaggccttc aacctgaatc tggcgaacac
     6481 gttcacgggt gaggccaatt tcacgaccta catcttccag tgttgccgct tcgtacccca
     6541 gcaaaccgaa tcgacgtgcc agtacttcac gctgtttggc gttcagctcg aacagccatt
     6601 tgacgatgct ctgcttcata tcgtcatctt gcgtggtatc ttccggaccg ttctcttttt
     6661 catcggccag gatgtccagc aacgcttttt cggaatcacc acccagcggg gtgtctaccg
     6721 aggtaatgcg ctcgttaaga cgaagcatac ggctgacgtc atcaactggc ttatccagtt
     6781 gctctgcgat ctcttccgca cttggttcat ggtccagctt atgggacaac tcacgtgcgg
     6841 ttcgcaggta aacgttcagc tcctttacga tgtgaatcgg caaacgaata gtacgggttt
     6901 ggttcataat cgcccgttca atcgtctggc gaatccacca agttgcgtat gttgagaagc
     6961 ggaaaccacg ttccgggtca aacttctcta ccgcgcggat cagccccagg ttgccctctt
     7021 cgataaggtc cagcaacgcc agaccacgat tgccataacg gcgggcaatt tttaccacca
     7081 gacgcaagtt actctcgatc atccggcggc gagaggcgac atctccacgc agtgcgcgac
     7141 gcgcaaaata aacttcttct tcggccgtta acagtggtga ataaccaatc tcaccaaggt
     7201 aaagctgagt cgcgtccaac acacgctgtg tggctccctg cgataacagt tcctcttcgg
     7261 ccaaatcgtt atcactgggt tcctcttcta ctaaggcctt ttcgtcaaaa acctcaactc
     7321 cgttctcatc aaattccgca tcttcattta aatcatgaac tttcagcgta ttctgactca
     7381 taaggtggct cctacccgtg atcccttgac ggaacattca agcaaaagcc tggttccgcc
     7441 gatttatcgc tgcggcaaat aacgcagcgg gtttacggat ttccccttgt aacgaatttc
     7501 aaaatgcaag cgtgttgaac tggttccggt gctacccatg gttgctattt tttgccccgc
     7561 cttcacttct tgttgttccc ggaccagcat tgtgtcgtta tgggcgtagg cactcaggta
     7621 atcatcatta tgtttgatga taatcagatt accgtagccg cgcagcgcgt taccggcata
     7681 aacaacgcgg ccatctgcgg tcgcgataat tgcctgtcct ttgctgcctg cgatatcaat
     7741 ccccttgttg cccccctcag aagcgccaaa ggtttcgatc actttgccct cagtcggcca
     7801 gcgccaggtg gagataggcg tactggttga tgtactgctg acagtcggct cggttgtgct
     7861 tgctgttggt accgttacag gcgctgtgac tgtggtcgca gttggcttgt tgttcggcaa
     7921 cattttgtta gcactctgtt cacccgaaga ctcagaatac gtaattgtcg gttgcgacgc
     7981 aacagcaacg gtggaatttt gtgcaggctt gatcacaact ccttgctctg ctgcgtcggc
     8041 ctgggtaatg gcatttccgc cagtgattgg cgtaccggaa gcattaccca cctgcaaggt
     8101 ctgaccaacg ttcagcgcgt atggtgcctg aatattgttg cgctgagcaa ggtcacggaa
     8161 atcgttgcca gtaatccagg cgatatagaa aagtgtgtcg ccttttttca cggtataggt
     8221 actgccgcta taactgcctt tcggaatgtt cccatactga cggttataga cgatgcgtcc
     8281 gttttccatc tgtaccggct gctgagctac tggctgcact ggctggattt gcggttgttg
     8341 agtagcctga atttgtggct gctgtaccgg ctgaatttgc ggttgctggg ctgtagacgt
     8401 cgtccccatt ttcggcggcg gcgtaatcaa cataccagaa ttagtatttg caggcgcatt
     8461 gccattaacg gagctgaccg gtgccggtgg atttgaagtg tcagaacagc ctgccagcca
     8521 tagcgaaacc agtgacaaag cagcaatgcg gcgaacggtg aattttgggc ttcccgcgct
     8581 catttatccc ccaggaaaaa ttggttaata accagtgaca taattaccgt gcaaggcacc
     8641 ctactgaaca ctggaaaaga tgttcacgat acgctgacct gcggcaaaat aaccaggaaa
     8701 aatccaggta tttcctcacg ttttaagcca gctcaccctt cactaaaggg acaaagcgca
     8761 cggcctccac ggtatcgata ataaattcgc ctccccgacg acgcacccgt ttcaaatact
     8821 ggtgctcctc ccctacgggt aagacgagaa tcccgccttc gtccagctgc gtcattagcg
     8881 cagttggaat ttccggcggt gccgccgtaa caatgatagc gtcaaacggc gcacgtgcct
     8941 gccaaccttg ccatccatcg ccatgacggg ttgaaacatt atgtaaatca agatttttca
     9001 ggcggcgacg tgcctgccac tgcaagcctt taatccgttc aaccgagcaa acatgctgga
     9061 caagatgcgc taggattgcc gtttgatatc ccgaaccagt gccaatttcc agcacccgcg
     9121 actgcggcgt cagctcgagt aattcggtca ttcgcgccac catatacggc tgcgaaattg
     9181 tctgcccctg acctatcggc aaggcgatat tgtcccaggc tttttgttca aacgcttcat
     9241 caacgaattt ttcacgcggc acggcggcaa gtgcattcag cacctgctca tcctgaatac
     9301 cttgcgcacg taattgctcc agaagtgctt gtacgcgtct gcttaccatt gcgtgccaac
     9361 tcccacgctg tttaaccagt ctgaaaccac atcttgcgcg ctatgcgcag ttaaatccac
     9421 atgcagcggc gtgatggaga catagccctc atctaccgca gcaaaatcgg tccccggacc
     9481 ggcatcacat ttaccgcccg gcgggccaat ccagtacagc gtattgccgc gcggatcttg
     9541 ctgcgggatc acctgatctg ccggatgtcg tgtaccgcag cgcgtcacgc gaataccttt
     9601 gatttgatcc aagggtaaat ccggaacgtt aatattaaga atacgtccgg tgcgcagcgg
     9661 ctctttacac agtgcgcgca aaattgaaca ggttaccgcc gcggcagtgt cgtaatgttt
     9721 atgcccgtca agcgagacgg ccagcgccgg aaaacctaaa tgacggcctt ccatcgcggc
     9781 ggctaccgta ccggaataaa taacgtcatc ccccagattc ggcccggcgt taatgccgga
     9841 cacaacaatg tccgggcgcg gacgcatcag agcattcacg ccaagataga cgcaatcggt
     9901 cggggttccc atttgcacag caatatcacc attttcaaag gtaaacgtgc gcagggagga
     9961 ttccagtgtc agagaatttg aagcgccgct gcggttacga tcgggggcga ccacctgaac
    10021 gtcagcaaac tcacgcaagg ctttcgccag cgtttgtata ccgggtgcat gtaccccgtc
    10081 atcattactc agcaatatgc gcataatcac ctgttgtgtt gataagttcc ctgacaacgc
    10141 tggttgcaaa actacccgcc ggaagccaga aacggatctc tacggtgacg tcatcccacc
    10201 aattccagct taattgttgc ggatacagca acatcgctct gcgcgcggct tcaacttttt
    10261 cgcgcaccag taaagcttgt aattcagttt ctgcggcgac agctgccttt tcgaatgcca
    10321 gcgcttcacg ctgagttccc cattcgccac tacccggcaa tgcggcggtt atcatcagct
    10381 ctttatcgtt gacgcgacgc tgtaattccg ccagttcttc ggtggttgcg acaaaccagc
    10441 taccacgtcc ggctaattgt agcgcatcgc cgtcaacaac ttgattaacg tctgcttttt
    10501 tgaggcgctc agcaacaatc tgattaaaca acgcactgcg ggctgccgac aaccaaaaac
    10561 tccgtttatt gcgatcgcgc accggagtat tggtttgcgc ccagcgctgc gccccctgca
    10621 agttgctacc gccaatccca aaacgttggg caccgaagta gttcggtaca ccttttacgc
    10681 aaatatcgat cagacgttgt tcaacgtcat cgcgattgct cacttcgcgc agaaccaggg
    10741 taaaggcgtt acctttcagc gcgcctaaac gcagcttgcg cttgtgccgc gcatactcca
    10801 gcacctggca gccttccagt tgaaaggcgc tcagatcggg catttccttg cctggcacgc
    10861 gagcgcataa ccactgttcc gtaacagcat gtttgtcttt ttgcccagcg aagctgactt
    10921 cacgggcatg aattttcagg aatttcgcca gtgcatccgc cacaaaacgg gtattgcagc
    10981 cgtttttgag gattctaacc agaatatgct caccttcacc atcaggctca aagcccaaat
    11041 cttccaccac cacaaagtct tccggattgg ctttcagcag cccggtgcct tgcggtttac
    11101 cgtggaggta agtgagatta tcaaactcaa tcattttgtt gccttaatga gtagcgccac
    11161 cgcttcacag gcaatccctt ccccacgtcc ggtaaatcca agtttttccg tagtagtggc
    11221 tttcacgtta acatcatcca tatggcagcc gagatcttcg gcaataaaca cgcgcatttg
    11281 tggaatgtgc ggcaacatct tcggtgcctg agcgataata gtgacatcga cgttgcccag
    11341 ggtataaccc ttcgcctgaa tacgacgcca ggcttcgcgt agcagctcgc ggctgtcggc
    11401 acctttaaat gccggatcgg tatccgggaa cagcttgccg atatccccca gcgccgccgc
    11461 gccaagcaat gcatcggtca acgcatggag cgccacgtcg ccatcagaat gcgccagcaa
    11521 tcctttttcg taaggaatgc gtacgccacc aatgataatt gggccttcac cgccaaaggc
    11581 atgtacgtca aaaccgtgtc caattcgcat tatgtattct cctgatggat ggttcgggtg
    11641 aggtaaaact cggccagtgc caaatcttcc gggcgcgtga ctttaatgtt atccgcacgg
    11701 ccttcgacca actgaggatg gaatccgcaa tattccagcg ccgaggcttc gtcggtaata
    11761 gtcgcgcctt catttagagc gcgcgtcaga cagtcatgta acagctcacg agggaaaaat
    11821 tgcggcgtca gcgcgtgcca taagccgttg cgatcaacgg tatgagcaat ggcatttttg
    11881 cccggttcgg cacgtttcat cgtatcgcgc actggtgcgg ctaggatccc tcccgtgcgg
    11941 ctggtttcgc tcaacgccaa caatcgcgcg aggtcatcct gatgcagaca aggacgagcg
    12001 gcgtcatgca ccaataccca ctgcgcgtcg ccagcggctt tcaaacctgc cagcacggaa
    12061 tcggcacgct catcaccgcc atctacaacg gtgatttgcg gatgattcgc cagaggaagt
    12121 tgtgcaaaac ggctatcgcc aggacttatg gcaatgacga cacgtttcac ccggggatgc
    12181 gccagcagcg catgcaccga gtgttcaaga atggtttgat taccgattga gagatattgc
    12241 ttaggacatt ccgtttgcat tcgacggcca aatccggccg ccggaaccac ggcgcaaaca
    12301 tccaaatgag tggttgccat gttaattccc gggctgattt atcgattgtt ttgccccgca
    12361 gactgtgcgc gcttcgacgc gtcaggcacc agacgataaa aagtttcgcc cggcctggtc
    12421 atgctgagtt cattacgcgc acgctcttcg agcgcctcct ggccgccatt gagatcgtca
    12481 atttcggcaa aaagttgatc gtttcgcgct ttaagtttcg cgtttgtagc ttgctgtgcc
    12541 gccacatcat cattgacgcg ggtatagtca tgtataccgt tcttaccgaa ccacagcgaa
    12601 tactgtagcc agaccagaat agccagcaac agcagcgtta gtttacccat cctgccccct
    12661 gaaaaacggc atcatcatcc catgcatccg aagacgactc tacatcctct gttggggata
    12721 ccgcgacaac gcgggcaaat gtaccacatt tgtccattgt tacgtatacc cagggcgtgc
    12781 agaacataat ctcattatta gttacggttt gaattatgaa cagaggagac aagaaagtac
    12841 aaattagccc agtagccaca taaacagtgc gccaaacata atgcctactg tcaccagggt
    12901 gaaaacaata ctgtagcgta gctttccgtc catcaatgaa tgcagcgcaa tccccaccac
    12961 taccgcgacg ggcatcagcg ccagaaagaa aggccaggtg tagataaaga agaacagcgt
    13021 gttagagcca taaatcaaca tcggcatcgc cagcgcaaat aaccaggaga taaaaccgac
    13081 cacggcacca ggcagtgacc atgtggtttc ttcatcctca gtaaggctgt cgttatttgt
    13141 tagtgtaatg ttatggctat tacgcatatt tgatcctgtt actttgacga accgggcatg
    13201 gaaacccggt ggtgtctcag gatctgataa tatcgttctg tctcaacaga tctaataatt
    13261 gctgtaccaa atttgttact aattgttcac cattgagatg aatttctgcc gattcaggcg
    13321 cttcgtaaac ggaatctatt cccgtaaagt tgcgcagttc accggcacgc gctttcttat
    13381 ataagccttt tggatcgcgg gcttcgcaaa tcgccagcgg cgtatcgaca aacacttcga
    13441 taaagcgccc ttctcctacg cgttcacgaa ccatctggcg ttcggcgcgg tgtggcgaga
    13501 taaatgcggt cagcaccacc agtccggctt caaccatcaa attcgccact tcaccgacgc
    13561 gacggatatt ctctttacga tcggcatcgc taaaaccgag atcgctgcat aatccgtggc
    13621 gaacattatc gccatccagc agatacgtac tgacgccgag tttatgtaac gcctcctcca
    13681 gcgccccggc gaccgttgat ttaccggacc cggagaggcc ggtaaaccac agcactacac
    13741 cacgatgacc gtggtgtagc tcgcgttgtt gcacagtgac cggatggcta tgccagacga
    13801 cgttttcgtc atgcagcgcc attatttatc ccccagcaaa tcgcgcgcgc cccagtgcgg
    13861 aaagtggcga cgaaccagag cattcaattc cagttcgaat gcactgaatt cagatggcgc
    13921 agcagttgcc tggctaactg gctcgtgcac cataccggca cccacggtca cattgctcag
    13981 gcgatcgata aaaatcagcc cacccgtcac cggattttgt tgataacgat ctaacaccag
    14041 cggctcgtca aaagtgagat ccacgaggcc gatcccattc agtggcaggt tttcaacttc
    14101 acgctgggta aggttattaa tatcaacctg atagcgaatg ccatcaacac gcgcgcgcgt
    14161 cttcttaccg gcaattttga tgtcgtaact ctgccctgga gaaagcggct gttccgccat
    14221 ccataccaca tccaccgacg cgctctgcac cgccggtaac gcttcgtctg ccgccagcag
    14281 cagatcgcca cggctgatgt cgatctcatc cgtcagcacc agggtgatcg cttctccggc
    14341 aaaggcttct tcgcgatcac catcaaaagt cacgatccgc gcgacgtttg attccacacc
    14401 agagggcagc acttttacac gttgcccgac ttccacgcga ccggatgcca gcgttccggc
    14461 gtagccacga aaatcgagat tcgggcgatt aacgtactgc accgggaagc gcattggctg
    14521 agcatccacc actcgctgga tctccacggt ttccagcact tcgagcagtg tcggaccgct
    14581 gtaccacggc atactttcac tttgcgatgc cacgttgtcg ccttccagtg cagagagcgg
    14641 cacaaagcgg atatccagat tacccggcag ctgcccggca aaggtcagat aatcttcacg
    14701 aatacgggtg aacgtctctt cactgtaatc caccagatcc attttgttga tcgccacgac
    14761 cagatgtttg atccccaaca gtgtggagat aaaactgtga cgacgggttt gatcgagcac
    14821 gcctttacgg gcatcgatca gtaagatcgc cagttcacat gtcgatgcgc cagtcgccat
    14881 attgcgagtg tactgctcgt gccctggggt gtcggcgata ataaatttac gcttctcggt
    14941 agagaaatag cggtaggcca catcaatagt gatgccctgt tcgcgctcag cttgcaggcc
    15001 gtccaccagc agagccagat ccagcttttc gccctgggta ccgtgacgct tactgtcgtt
    15061 atgcagcgat gagagctgat cttcatagat ttggcgagta tcgtgcagca ggcgaccaat
    15121 cagagtgctt ttgccgtcat cgacgctgcc acaggtcaga aaacgcagca ggcttttatg
    15181 ttgttgcgca atcatccagg cttcgacgcc gccttcattg gcgatttgtt gtgcaagtgc
    15241 ggtgttcatc ttaaaaatac ccctgacgtt ttttcagctc catagatccc gcctggtcgc
    15301 ggtcaatcac gcggccctga cgttcactgg tggtggaaac cagcatctct tcgatgattt
    15361 ccggcagtgt ttgtgcattt gactccaccg caccggtcag cggccagcag cccagcgtac
    15421 ggaaacgcac catccgtttt ttaatcactt cgcccggttg caggtcgata cggttgtcat
    15481 caatcatcat caacataccg tcgcgttcca gaaccggacg ttccgcagcg agatacagcg
    15541 gcacaatgtc gatattttcc agccagatgt attgccagat atcctgctcg gtccagttag
    15601 agagtgggaa gacgcggatg ctttcgcctt tgttaatttg cccgttgtag ttgtgccaca
    15661 gctccgggcg ctgatttttc ggatcccagc gatggaagcg gtcacggaaa gagtagattc
    15721 gctctttagc gcgggatttc tcttcgtcac ggcgcgcacc accgaaggcg gcatcaaaac
    15781 cgtatttgtt cagcgcctgt ttcaggcctt cagttttcat aatatcggta tgtttcgcgc
    15841 tgccgtgcac gaatggatta atccccatcg ccacgccttc cgggttttta tgcaccagca
    15901 gctcgcagcc gtaggctttc gccgtacgat cgcggaactc atacatctcg cggaatttcc
    15961 agccggtatc gacatgcagc aacgggaaag gcagcgtacc tggataaaac gccttgcgcg
    16021 ccaaatgcag catgacgctg gaatctttac cgatagagta gagcatcacc ggatttgaga
    16081 attctgccgc cacctcgcga ataatgtgga tgctttccgc ctccagttgc cgcaggtgag
    16141 taagtcgtat ttgatccata accgttcctt tgcaataccg ctattttctt gccatcagat
    16201 gtttcgacta tagggagcgt aagagaacga atgaaattac caattagaat gagtagttcc
    16261 ttaacggaat aacgatttgg caaagctaat atcaaaaagt gcttaaggca ccggatttct
    16321 ggcgtttagg aagatttgaa attgttttag cgcagcggaa gtttcatact atggcggtaa
    16381 aaaaatttgc atggtattta aggactcact atgttttccg cattgcgcca ccgtaccgct
    16441 gccctggcgc tcggcgtatg ctttattctc cccgtacacg cctcgtcacc taaacctggc
    16501 gattttgcta atactcaggc acgacatatt gctactttct ttccgggacg catgaccgga
    16561 actcctgcag aaatgttatc tgccgattat attcgccaac agtttcagca aatgggttat
    16621 cgcagtgata ttcggacatt taatagtcgg tatatttata ctgcccgcga taatcgtaag
    16681 agctggcata acgtgacggg aagtacggtg attgccgctc atgaaggcaa agcgccgcag
    16741 cagatcatca ttatggcgca tctggatact tacgccccgc tgagcgatgc tgacgccgat
    16801 gccaatctcg gcgggctgac gttacaagga atggatgata acgccgcagg tttaggtgtc
    16861 atgctggaac tggcagaacg cctgaaaaat acgcctaccg agtatggtat tcgatttgtg
    16921 gcgaccagcg gcgaagagga aggaaaatta ggcgctgaga atttactcaa gcggatgagt
    16981 gacaccgaaa agaaaaatac gctgctggtg attaatctcg ataacttaat tgttggcgat
    17041 aaattgtatt tcaacagcgg tgtaaaaacc cctgaagcag taaggaaatt aacgcgcgac
    17101 agggcgctgg caattgcgcg cagtcacgga atagccgcaa cgaccaatcc gggtttgaat
    17161 aaaaattatc cgaaaggcac tgggtgttgt aatgacgcag aaatattcga caaagcgggc
    17221 attgctgtac tttcggtgga agcgactaac tggaatcttg ggaataagga tggttatcag
    17281 caacgcgcaa aaacagccgc attccctgcg ggaaatagct ggcatgacgt aagactggat
    17341 aatcaacagc atattgataa ggcacttcct ggaagaatag aacgtcgctg ccgtgacgtt
    17401 atgcggataa tgctacctct ggtgaaggag ttggcgaagg cgtcttgatg ggtttgaaaa
    17461 tgggagctgg gtgttctacc gcaggggcgg ggaattctaa gtgatatcca tcatcgcatc
    17521 cagtgcgccc ggtttatccc cgctggcgcg gggaactctt actgcttggt atgcggaatc
    17581 acaccctgaa cggtttatcc ccgctggcgc ggggaacaca ctaagcatac ataactgttt
    17641 ttaaacaaat ttattccgca tcaacaatct accaactaaa ttaaaacatt tccttatttt
    17701 taaagaacgg ataacttatt gattatcaac aggaagaaaa gaaaccaaac gtaacccatc
    17761 caaatccacc ggaatacgtc tgttttctcc ccaggtctga aattcaaaac ccgactcgat
    17821 attggtcgcc caggccatca ccacatttcc gcaaccagcc agttgggtaa tttgctgcca
    17881 gatcatctcc cgaatacgtt ttgatgtatc accaacatac acaccggcac gcacttccag
    17941 tagccagatt gcgagccgtc cacgtaagcg cggagggaca ttttctgtaa caaccacgac
    18001 catgctcatc cgccgcgccc ccggtgacca ctatcaccca gcgtttcagg ttcagggatg
    18061 gcaggcggta acatatccgg cgcgggttgt ggtggttcaa tttcacctgc agcaaggact
    18121 tcctcaatta acggtattaa tttgcccgtt aacttagtgc tacggaaaat atcgcgacag
    18181 gctaatctga cttctttatc aggttctgcg ggttgcctcg ctgctatttc aaatgccttt
    18241 ggcacaaccg aatcaaattt aatgatatcg gctatgtcat aaacaaatga aagcggtttg
    18301 ccactatgga taaacccaat agcgggcgca tatcccgcgg ctaatactgc cgcttcagat
    18361 ataccgtaca gacatgatgt agcagcactg atgcagcgat tcacaacatc gcctttttcc
    18421 cagtctttag gatcgtattt gcgaccattc catttcacac catattgttt cgccagtaat
    18481 gcatacgtct ggcgaacgcg agatccctca attccacgta actgctccac tgaacggcga
    18541 gctggcggtg gttcacgaaa acgtaattca tacattttgc gcaccacctt caaccgtaga
    18601 tcttccgtta aagccagctt tgcctagtag agtaatttat ctgcccgcgc ccctccgggt
    18661 tgtccggagg agtaaacgcg aacgcccgct tcaccgaccc agaccagcag tgttcccacc
    18721 gtggcggcca gatggactgc cgcgtgggaa actcttgttc ccggttcgag cataatgcag
    18781 gcgaccgatc ccactggaat atgcgtgcgg atcccggttt tgtcgatcag cacgaaagcg
    18841 ccgtccagta cgtcgatttg accgtactgg aggaagatca tggaagtgcg atcttttaat
    18901 gggatcgggc tcagcggtac aaacgtcacg cctctgctcc gggtttgatc agcatcagac
    18961 cacaaccgaa cgcccggctt ttgccatacc cctgactgag acgttgcaga aataacccag
    19021 gagcggtgac cgtaagcatc cccgtataat ccacgctact gaactggatc agttgccggg
    19081 agttttcccg ccgcagttgc tgttgtcggt aggcatcaac cgaagtatcc agcagtgtaa
    19141 aaccgcttct ctccccctgc gctgccagcc agtccagcgc agcctgttgt tgatgcaacc
    19201 agacatcact tccttccacc tgccccctca cctgccgttt cgcctccatc agcaagtcgt
    19261 ggcgtttgcc cgctttacaa attgttggat tcgcccgcag gttgaaacaa agggattgtc
    19321 cggtacgcaa ctctggcgca aatggtcggc attcgatggt gaaaatatca ctttccgccg
    19381 ggcgttcctg cgacaggaca aaaaagcg
//
LOCUS       DAEEKQ010000071        18844 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.071, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000071 DAEEKQ010000000
VERSION     DAEEKQ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.071"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(253..2124)
                     /gene="ppiD"
                     /locus_tag="KLK57_002358"
     CDS             complement(253..2124)
                     /gene="ppiD"
                     /locus_tag="KLK57_002358"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HBE0892709.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(2316..2588)
                     /gene="hupB"
                     /locus_tag="KLK57_002359"
     CDS             complement(2316..2588)
                     /gene="hupB"
                     /locus_tag="KLK57_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HBE0892710.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(2797..5151)
                     /gene="lon"
                     /locus_tag="KLK57_002360"
     CDS             complement(2797..5151)
                     /gene="lon"
                     /locus_tag="KLK57_002360"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HBE0892711.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(5339..6613)
                     /gene="clpX"
                     /locus_tag="KLK57_002361"
     CDS             complement(5339..6613)
                     /gene="clpX"
                     /locus_tag="KLK57_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HBE0892712.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(6739..7362)
                     /gene="clpP"
                     /locus_tag="KLK57_002362"
     CDS             complement(6739..7362)
                     /gene="clpP"
                     /locus_tag="KLK57_002362"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HBE0892713.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(7608..8906)
                     /gene="tig"
                     /locus_tag="KLK57_002363"
     CDS             complement(7608..8906)
                     /gene="tig"
                     /locus_tag="KLK57_002363"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HBE0892714.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(9250..9567)
                     /gene="bolA"
                     /locus_tag="KLK57_002364"
     CDS             complement(9250..9567)
                     /gene="bolA"
                     /locus_tag="KLK57_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HBE0892715.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            9872..10450
                     /locus_tag="KLK57_002365"
     CDS             9872..10450
                     /locus_tag="KLK57_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0892716.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            10494..11969
                     /gene="ampG"
                     /locus_tag="KLK57_002366"
     CDS             10494..11969
                     /gene="ampG"
                     /locus_tag="KLK57_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HBE0892717.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPTGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            12430..13377
                     /gene="cyoA"
                     /locus_tag="KLK57_002367"
     CDS             12430..13377
                     /gene="cyoA"
                     /locus_tag="KLK57_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HBE0892718.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            13399..15390
                     /gene="cyoB"
                     /locus_tag="KLK57_002368"
     CDS             13399..15390
                     /gene="cyoB"
                     /locus_tag="KLK57_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HBE0892719.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            15380..15994
                     /locus_tag="KLK57_002369"
     CDS             15380..15994
                     /locus_tag="KLK57_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HBE0892720.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            15994..16323
                     /locus_tag="KLK57_002370"
     CDS             15994..16323
                     /locus_tag="KLK57_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HBE0892721.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            16335..17225
                     /gene="cyoE"
                     /locus_tag="KLK57_002371"
     CDS             16335..17225
                     /gene="cyoE"
                     /locus_tag="KLK57_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HBE0892722.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGFAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            17410..17721
                     /locus_tag="KLK57_002372"
     CDS             17410..17721
                     /locus_tag="KLK57_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HBE0892723.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            17705..18247
                     /locus_tag="KLK57_002373"
     CDS             17705..18247
                     /locus_tag="KLK57_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0892724.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(18303..>18844)
                     /locus_tag="KLK57_002374"
     CDS             complement(18303..>18844)
                     /locus_tag="KLK57_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HBE0892725.1"
                     /translation="GIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATCRKDMID
                     RKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSIRRPSGK
                     EINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTTIVAATA
                     TINGKDVELLKAGAIEQKW"
BASE COUNT         4215 a         4953 c         4581 g         5095 t
ORIGIN      
        1 cgccgtcggt ttcgccacag aagctgccgc cagcgcgcta tgagacattc cggcacaggc
       61 cagggacagg gtaatgagca gtgctttaat tccgtgtttc atactgtttt ctccttgttt
      121 gttaacagtg gaatcacctt aacgggagtg aaaacagtaa acaaatggca atgttcagaa
      181 atggaaaagg ccgcgaaagc gaccttttct tgttacagct gcaacgcatt tttttgcgag
      241 gcggcttcag gattattgct gttccagcgc atcgccaatt ttgattttcg cctctttacg
      301 caggttactc atcagagctt caaagacgat ttgtgcgttg ttctgggtga taccctgcac
      361 catcgctttt ttctgatctt ccggcattga accttgtttc acttcatcca gcgccagcag
      421 aaccacatta ccttgcatat cggtcgccat accgtagctc ggtttgtctt tcgctggcag
      481 tggcagtgca aacgccgcct ggctaatcgg gtcacgaccg gaacggctta aggttttcgg
      541 ctcgccaaat ttcagaccgg cagcctgcat agcttccgcc cctttgccgg ctttcaaatc
      601 aaccagcagt ttctcagcat ccactttcgc ctgttgttca gctttgttgt gctgaaccag
      661 tgccttaact tgttcctgaa catctgccaa cggtttcacc gcttccggtt tgtgctcgct
      721 gatgcgcagc acgaatgcgc ggtcgccgtc tacggtgatg atgtcagagt tgatgcccgg
      781 cgcgccgttt tcacctacca gaccgccgtt aaagatagcg tcggcaaccg gcttgaagtt
      841 caactcttcc ggcaggttat ctttgctgaa ccaacccgtc tgagtggctt taacgccggc
      901 agcttgctct gcaccggcca gagactcggt gtcgttgctt gccgcatcgc tcactttctg
      961 ctgcagcgca tagtacgcat cgagggcttt ttcgtgtttc actttcgccg caatgtcgtc
     1021 acgtacttcg tctaacgatt tcactttcgc tggctgaatg tcgtccagac gtacaatcag
     1081 gaaaccgacc gaagatttga tgacaccaga cagttggcct ttttctttca gaccagcatt
     1141 tttcagttcg tcggggatag tggcatcttc taaccaaccc atatcgccgc cgttacgggc
     1201 agagataata tcggcagatt tttctttggc taatgcagca aaatcaccgc ctttattcag
     1261 ctcatcaagt accgctttcg cttcatcttc agttttggtc tggatgatgc tgtagcgggt
     1321 acgctgcggc tgggtgaatt gatcctgatg ctgatcgtag tagctctgga tatccgcatc
     1381 gctaaccggt tgctgcatcg ttgcggcatc cagcttgatg taactcacgc ggaattgttc
     1441 cggcgtcatg aaattgtttt tgttttgttc gtagtagctg gcaatctcct gttcggtcac
     1501 aggctgcttc gccgccagcg cgttaacatc gatagtcgcc tcacgcacca cgcgttgttg
     1561 cgcgaccagt gccgccagct cgtcggtttc acctttcagc ataaaatcgg taccggcaac
     1621 accgttaatc agctgttggg tagtgagctg gttacgcagc gcctgggcgt actgatcggc
     1681 ggtcatcccc atctggttga ggataccgtt atagcggctg ttatcaaatt tgccgtcaac
     1741 ctggaaggct ggggtcgcga aaatcgcctg tttaacctgc tcatcactga tacccagttt
     1801 cagctcacga gcgtactgat ccaacagcgc ctcgtcgatc agacgattca gcacctgttg
     1861 acgcagggtt ttcatatagc cttcgttcgc tgccagctcg gagtattgat cgcccagctg
     1921 ttgctgcatg cgattacgct cgctgttaaa ggcgttctcg aactgcccac ggctgatttc
     1981 ctggtcattc acttttgcgg cgtagttatt gcctccgcca atcaggtaac cactcacgcc
     2041 ggtcaatatg aacgacacga taatgatacc gaaaataatc ttgagcacga gactgtttgc
     2101 agccgtgcgt aagctgtcca tcatggtgta acaacactcc gctgtagatg actgtgtacc
     2161 tcacgcaacg tatcgatgcg cgcaaaggcc tattgtgaca agaaacgggg gcaattgtca
     2221 gcccacaaga cacagaaagt caccgaatac aaataaaaaa ggcacatcag tagatgcgcc
     2281 cttgaacttc gtcacatccc cactggggac aacgcttagt ttaccgcgtc tttcagtgct
     2341 ttacctgcac ggaagctcgg tactttagcg gcagcgatgg tgatctcttt accggtctgc
     2401 gggttgcggc cagtacgggc agcacgctct ttaacggcaa aagtaccaaa acctaccagt
     2461 gctacatcat ccccttcttt cagagattca gttacggaag caataatagc atctaacgca
     2521 cggccagccg cagctttaga gatatcagcc cctgcagcaa tcttgtcgat caattgagat
     2581 ttattcactc ttctcttcct ctttataatt tatatcgcac ttgaatcctt caaggtacga
     2641 acgcgcagca gttatatcag gccagccatc cccttacaac accccttaat aagggcaagc
     2701 ccgatccgcc atctaactta gcgagacaaa aaaaggctgg caagcccgaa ttagcctgcc
     2761 agccctgttt ttattagtgc attttgcgcg aggtcactat tttgcagtca caacctgcat
     2821 gccagacggt tcattttgca gcgccagagt cagaacttcc tcaatgcgct tcacaggatg
     2881 aatgtccaga tcggcaatta cgttgtcagg aatctcttcc agatcgcgtt tattttcgaa
     2941 cggaattagc actgttttaa tcccgccgcg atgcgctgcc aggagttttt ctttcaaacc
     3001 accgatcggc agtacctgac cacgcagagt gatctcaccg gtcattgcca catcggcacg
     3061 aaccgggtta ccggtcaggc aagaaaccag cgcggtgcac atagcaatac cggcactcgg
     3121 accatctttc ggcgtcgcac cttccggtac gtggacgtgg atgtcacgtt tttcgtaaaa
     3181 atcagggttg atccccagtt tttccgcacg cgcacgaacc accgttaacg ccgcctgaat
     3241 agactcctgc atcacttcgc cgagcgaacc ggtataggtc agtttgcctt tacccggaac
     3301 acatgcggtt tcaatggtca gcaagtcacc gcccacttcc gtccacgcca gaccggttac
     3361 ctgaccgaca cggttttcgt tatcagcgcg accatagtcg aaacgctgaa caccgaggta
     3421 gtcatgcagg ttatcgccgt taatttcgat atgttttaat gacttatcga gcagtaactg
     3481 cttaaccgct ttgcggcaca gtttggagat ttcacgctcc agaccacgca cgcccgcctc
     3541 acgggtgtag taacgaataa tgccgataat ggcgctatcg tcgacggtca gctcaccttt
     3601 tttcagtgca ttacgttcaa tctgcttcgg cagcaggtga cgtttggcga tgttcagttt
     3661 ttcatcttcg gtataaccgg agaggcgaat cacttccata cgatccagca gcggtgccgg
     3721 aatgttcatg gagttcgacg tcgcgacaaa catcacgtcg ctgagatcgt aatccacttc
     3781 caggtagtgg tcgctgaacg ctacgttctg ctctggatcc agcacttcaa gcagtgcaga
     3841 ggccggatcg cctcgcatgt cagaagacat tttgtcgatc tcatcgagca ggaacagcgg
     3901 gtttttcacg cccactttcg ccattttctg gatcagttta cccggcatag aaccgatgta
     3961 agtacggcgg tgaccacgga tttccgcttc atcacgcacg ccgcccagcg ccatacggac
     4021 atatttacgc ccggtggctt tggcaatgga ctgaccaaga gaggttttac ctacccccgg
     4081 cggccctacc aggcagagga tcggtccctt gattttgttg acacggcttt gaaccgcaag
     4141 gtactcaagg atgcgatctt tcacgcgctc cagaccataa tggtcggtat caaggatttc
     4201 ctgcgcctga cgcaggtctt ttttgacctt gctgcgcgca ttccacggta cctgtaccat
     4261 ccagtcgata taaccacgca ctacggtcgc ttctgccgac atcggagaca tcattttcag
     4321 cttctgcaac tctgcttccg ctttctcttt tgcctctttc ggcatcttcg ccgcgtcgat
     4381 tttgcgcttc agggcttcgt tttcgtccgg cgcgtcgtcc atttcaccga gttctttctg
     4441 aatagctttc atttgctcgt tcagatagta ctcacgctgg gatttctcca tctgcttttt
     4501 aacgcggttg cgaatgcgtt tctcaacctg cagcagatcg atttccgatt ccatcattgc
     4561 catcagatat tccagacgtt cgttaacgtc ggacatctcc agaacggact gtttgtcagc
     4621 cagtttcagc ggcatatgtg cagcaatggt atccgccaga cgcgccggat cgtcgatgct
     4681 attcagcgac gtcagcactt ctggtgggat ttttttgttc agcttgatgt agccttcgaa
     4741 ctggctgatt gcagtacgca ccagcacttc ctgttcccgc tcatcaatgg tcggcgactc
     4801 cagatactcc gccttcgcag aaaagtgttc gccattgtca gagagcgcag aaatacgcgc
     4861 gcgctgtaac ccctcgacca gcactttgac ggtgccgtca ggcagtttca gcatctgcaa
     4921 tatagaggcc acggtcccga cggtgaaaag atcgtttaca cccggctcat ccgttgaagc
     4981 ttctttctgc gcgaccagca taattttttt atcatggtcc atcgccgctt ccagacaacg
     5041 gatagatttt tcccgcccga caaataaggg gatgaccatg tgcggataaa ccaccacatc
     5101 gcgcagcggc aatacgggga tttcaatgcg ttcagaacgc tcaggattca tagagctctc
     5161 tcttagttta atttccgcca ggtaatcaga tgacacgact gtgcttcacg ccatctatta
     5221 acatgtacgt cagtatatgg ggatgtttcc cccacattca acgccgagaa tagaggaaaa
     5281 attaaagggg agataaaatc cccccttttt ggttaactaa ttgtatgggt atggttaatt
     5341 attcaccaga tgcctgttgc gcttccggct taccataaat cagcaacggt ttgctttgac
     5401 catcaattac cgactcgtcg ataaccactt tttcgacgtc ttccatggac ggcagatcgt
     5461 acatggtatc gagcagtgcg gcttctacga tggaacgcag gccacgggca ccggttttac
     5521 gcgccatcgc tttcttagcg atagcatcca gcgcctcgtc acggaattcc agatccacgc
     5581 cttccagatt aaacagcgcc tgatactgct tggtcagggc gtttttcggc tctttgagga
     5641 tctgaatcag agcttcttcg ctcagttcat tcaacgttgc tacaaccggc agacgaccaa
     5701 tgaactcagg gataagacca aacttgatca gatcttccgg ttcaacctgc gccagcagct
     5761 cgccttcgct tgctttgtcg gacttcgctt ttaccgtcgc gccaaaacca atgccggagc
     5821 cggtttctac acggtgggaa atcactttat ccagaccggc aaacgcaccg ccacaaataa
     5881 acaggatctt agaggtatca acctgcaaga attcctgctg cggatgttta cgcccacctt
     5941 gcggtggaac agcagctacc gtaccttcga tcagtttcaa cagtgcctgc tgtacgcctt
     6001 caccggaaac gtctcgggta atggacgggt tgtctgactt acgagaaatc ttgtcgattt
     6061 catcgatgta gacaatcccg cgctgcgctt tctgtacgtc gtaatcgcac ttttgcaaca
     6121 gcttctgaat gatgttttca acgtcttcgc ccacataacc ggcttcggtc agcgtggttg
     6181 cgtcggccat ggtgaacggg acgtccagca ggcgcgccag cgtttcagcc agcagcgttt
     6241 taccggaacc ggtcggaccg atcagcagaa tgttactttt gcccaactcg acaccattgc
     6301 tggtatcgcc gttgcgcaga cgtttgtaat ggttgtatac cgcgaccgcc agcacttttt
     6361 tcgcctgttc ctggccgata acgtaatcgt ccaggtggtt gcgaatttca tgcggcgtcg
     6421 gtagcgcact gcgttcacga tgcggtgcaa cttctttaat ctcttcgcga atgatgtcgt
     6481 tacataaatc aacacattcg tcgcagatat acacggatgg accggcaatc agcttgcgca
     6541 cttcatgctg gcttttgccg caaaaagagc aatacagcaa tttgcctgag ccatctttgc
     6601 gtttatctgt catgggtcaa aacctcttct ttgttctttg tgccgcacac gacgacgcaa
     6661 atgccattct caggcgcaag ccgcttacag cgttgtgccg ccctggataa gtatagcggc
     6721 acagttgcgc ctctggcatc aattacgatg ggtcagaatc gaatcgacca gaccgtattc
     6781 caccgcttca ggggcggaaa ggaagcgatc gcgctcggta tcacgttcaa tctgttctaa
     6841 tgattgaccc gtatgaagcg ccataagttc attcatgcgc cctttaactt tcagaatttc
     6901 acgggcatga atttcgatat cggtcgcctg gccctggtag ccgcccaacg gttggtgaat
     6961 catcacgcgc gaattcggca ggcaaaaacg tttacctttt gcccctgcgg tcagcaagaa
     7021 agcgcccatc gaggccgcct ggcccataca gatggtgctg acatcaggct tgataaactg
     7081 catggtgtca tagatagaca tcccggcagt gatcaccccg cctggggagt taatgtacag
     7141 atagatatct ttttctgggt tttccgcttc taggaacagc atctgcgcca caatcaggtt
     7201 cgccatgtgg tcttcaacct ggccagtcag aaaaatgacg cgttccttaa gtagacgaga
     7261 atagatatca aaagagcgct caccgcgtga ggtctgttca atgaccatcg gcaccagcgc
     7321 catatggggt gcaaagttat ctcgttcgcc gctgtatgac atttccgtct cctggataaa
     7381 attgaaaaaa cctgctgtac cgattgtaac cttatggacg gattatcagc tagtccctgt
     7441 gttatgggta cggcatcacc atatttcaag cataacaatc ttttgttgtt acgctaacac
     7501 cgaaagaggc ttttcgcacg gttccatgca aaattcatga caaaaaaaag cccaccacct
     7561 ggcggtgacg ggcctttgtg cgaatttagc gcgttatgct gcgtaaatta cgcctgctgg
     7621 ttcatcagct cgttgaaagt ggtttctttt tcagtcactt tcgctttcgc cagtacagct
     7681 tcaacagcct gttcttccag agcaacattg cgcatgttgt ccatcagttc tttgtttttg
     7741 ctgtagaact cgataacttc tttcggatct tcgtacgcag aagccatctc ttcgatcagg
     7801 cctttcacgc gctcttcgtc agctttcagt tcgttggtgc ggataacttc gcccagcagc
     7861 aggccaacaa ctacgcggcg tttagcctgt tcttcgaaca gttcgcgcgg cagttccaga
     7921 gcttgttttt cgttgccacc gaaacgctgt gcagcctggc gacgcagaac gtcgatttcg
     7981 ctgtcgatca gcgcagccgg tacgtcgatg tcgttagctt ttaccagacc ttcgatcgcc
     8041 tgagacttaa cgcggttacg gatggcgctc ttcagctcgc gctccatgtt tttacgcact
     8101 tcagcgcgca gaccttctac ggaaccatct tcaacgccga aacgtttgat gaattctgca
     8161 gtcagttccg gcagttcacg ctcttcaact ttcttcaggt tgatagcgaa tttcgctgct
     8221 ttacctttca gattttctgc gtggtattct tccgggaagg tcacgtcgat ggtgaactct
     8281 tcgccagctt tgtggccttt gataccgtct tcaaagcccg ggatcatacg accctggccc
     8341 atcgccagta cgaaatcaga cgctttaccg ccttcgaact cttcgccgtc tacagaaccg
     8401 gtgaagtcga tggttacgcg gtcttctgct tcaacggcgc cgtctttttc tttccaggtc
     8461 gcctgctgtt tacgcagagt atccagcatg ccgtcaacgt cagcgtcggt cacttcaacg
     8521 atcggttttt caacttcgat cgcttccaga ccctgcagtt caacttccgg ataaacttca
     8581 aactctacag agtaggtgaa gtcttcaccc agcttgtatt cgcccggaac ataagtcggt
     8641 gcgccagccg gattgatttt ttctttaatg atggcgtcaa tgaagttacg gctcatcagg
     8701 tcacccagaa cgtcctggcg tacagacgcg ccataacgct gagcaacgat attcattggc
     8761 actttgcctt tgcggaagcc gtcaatacgt acttttttcg caacgttgac cagctcgctt
     8821 ttaacagcgg tctcgatgct gtcagcagcg atagtaatcg ttacacggcg gccaaggcct
     8881 tgagtggttt caactgaaac ttgcatcttg ttacctcaaa aaatcacagt gctcggtcaa
     8941 ctcttacccc gcaagcacag gttgttggct tcgcggaacc gggatgttct tataatcaat
     9001 cacattccct gttaccagaa tcatcccgaa agcattcaaa taggacgcgg cattatagcg
     9061 gcatcacttt tatgagtcga gaacggttat tgcgcgctgt cgcgtatttt ttcacaattc
     9121 ccgggcaatt ttcgtctgaa tgctgggcaa aaagcgcaaa atccagacaa aacaaaacgg
     9181 cccgcaggcc gtttttcata aaatctttag caacatactg gaaaagcgcc gacagttgca
     9241 aatgcgtttt tacgcgatgc ttcctgctcc acgacaggga ggagaggcaa agacggtgtc
     9301 ctgcaaccct tcccactcct taatagtgta agtatgcaga gccagcgcat gaacggtagt
     9361 agagagttcc tccgctaaag tactgtaaat cattcgatga cgattcagaa aacgttcacc
     9421 cgtaaaacga tcgctgacca gcacaacttt aaaatggctt tcagagccgg ctgggacatt
     9481 gtgacgatag ctttcatcca ctacttcgag gaatacgggt tggaacgccg cccttaattt
     9541 ttcttctatc cgctcacgta tcatcatgaa atatctcctc cgacaacgct gagatgtcat
     9601 ccatcccttt aaatactagc cgcttttacc gtttccattg cagcttaaca acaaatattt
     9661 aggtttcgtc ctggtttttc cgtcaagcgt atgaagttta ctgatttaaa gattgaaaca
     9721 caggcctcgc atcagcgttt cttttaccag gggtgattat ttcgccaaca tgatgaattt
     9781 acatgcctta cccacttccc ctcgccgttg gcaatgttat gatggcggga atttttacca
     9841 aacacgctta agagtctctg agagcccgaa catgttcaaa aaaatcctct tcccgttagt
     9901 tgctctgttt atgcttgcag gatgcgcaaa accgccaaca actattgaag tttccccgac
     9961 gattacactg ccacagcagg atccaagcct gatgggcgtc accgtaagca ttaatggtgc
    10021 cgatcagcgt accgatcagg cgctggcaaa agtcacccgc gataatcaaa tcgttaccct
    10081 gaccgcctcc cgagatctgc gtttcctgct gcaagaagtg ctggaaaaac agatgaccgc
    10141 gcgtggttac atggttggtc cgaatggccc ggttaatctg caaatcatcg ttagccaact
    10201 gtatgctgac gtgtcccagg gcaacgtgcg ctacaacatc gcgaccaaag cggatatcgc
    10261 catcatcgct accgcgcaga atggcaacaa aatgaccaaa aactatcgtg ccagctacaa
    10321 cgttgaaggt gcgttccagg cctccaacaa aaatatcgcc gatgcggtta acagtgtgct
    10381 gaccgatacc atcgctgata tgtctcagga caccagcatc cacgaattca tcaagcagaa
    10441 cgcgcgttaa tttctgccct ctggcccggt gcaaaccggg cctgtagacg cccatgtcca
    10501 gtcaatattt acgtattttt caacagccgc gttcagccat attgctgatc ctgggttttg
    10561 cttccgggct accgctcgcc ctgacatccg gtaccttaca ggcctggatg acggtcgaga
    10621 atattgatct caaaaccatt ggtttcttct ctctggtagg ccaggcttac gtttttaaat
    10681 tcctctggtc accgctgatg gaccgctaca cgcccccatt ttttgggcgg cggcgcggtt
    10741 ggctgctcgc cacgcaaatc ctgttattag tcgccattgc ggcgatgggt tttctcgaac
    10801 ctggcaccca actccgttgg atggcggcac tggcggtggt gatcgctttt tgctcggcct
    10861 cccaggatat tgtcttcgat gcgtggaaaa ccgatgtgct cccggcagaa gaacgtggtg
    10921 cgggcgcggc aatcagcgtg ctgggttacc gtttagggat gctggtttcc ggcggcttag
    10981 ccctgtggct ggcagataaa tggctgggct ggcagggcat gtactggttg atggcggcgc
    11041 tgttgatccc ttgtattatc gcgacgttgc ttgcaccaga accgaccgac accattcctg
    11101 tgccaaaaac gctggaacaa gcggttgttg cacctctgcg agatttcttt ggtcgcaata
    11161 atgcctggct tattttgctt cttatcgtgc tgtataagct gggcgacgca ttcgccatga
    11221 gcctgacaac cacgtttttg attcgcggcg tcgggtttga tgcgggtgaa gtaggcgtgg
    11281 ttaacaaaac gcttggctta ctggcgacca ttgtgggcgc attgtacggt gggattttga
    11341 tgcagcgcct gtcgctgttc cgggcgctgc tgattttcgg cattttacaa ggtgcgtcta
    11401 acgctggtta ctggctgctg tcgattactg ataagcatct ctacagcatg ggcgcagccg
    11461 tctttttcga aaacctctgt ggcgggatgg gcacatcagc ctttgtcgca ctgttaatga
    11521 cgctgtgtaa taagtcattt tccgctactc aatttgccct gctctcagcg ctttccgctg
    11581 taggacgagt ttatgtcggc cccgtggcgg gttggtttgt tgaagcacac ggctggtcga
    11641 cattctatct attctccgtc gccgctgccg taccggggct tattttgctg ctggtttgcc
    11701 gccagacgct tgaatataca cgagtaaatg acaactttat ctcccgtacc gaatatccga
    11761 caggttatgc ctttgccatg tggacactgg cggcgggcgt cagcctgttg gccgtgtggt
    11821 tactgctgtt gacgatggac gcgctggatt tgacgcactt ctctttcctg cctgctctgc
    11881 tggaagtcgg ggttttagtc gccctttctg gcgtcgtgct tggtggtttg ctggattatc
    11941 tggctctacg aaaaacgcat ctgacgtaat ctgtaaatat tatttagctg tagtaatcac
    12001 tcgccgtaat tattacggct aaataaacat cagtatcgct taataatatt tgttcttttg
    12061 tgcggcttag cgtttggaaa ttattgacgc aatttatgaa ttctattttt tcttacacga
    12121 ttcagctaat gagtctttat tttcccccca cccaattgtc accataatga taattatttg
    12181 ttaaacaatt gttttattca agattggtta taccaattgc ccgtccaaac tccttctgca
    12241 ctcccctttc gttataacgc ccttttgcaa cagcttctta aaatcaactt gatatgtttt
    12301 gcaacatatg taacctgaca gccaaatcca agtaacagga atttaatcat gtttacagta
    12361 atgtaacctc cccgtaaaat gcccacacac tttaaacgcc accagatccc gtggaattga
    12421 ggtcgttaaa tgagactcag gaaatacaat aaaagtttgg gatggttgtc attatttgca
    12481 ggcactgtat tgctcagtgg ctgtaattct gcgctgttag atcccaaagg acagattggt
    12541 ctggagcaac gttcactgat actgacggca tttggcctga tgttgattgt cgttattccc
    12601 gcaatcttga tggctgttgg tttcgcctgg aagtatcgtg cgagcaataa agatgctaag
    12661 tacagcccga actggtcaca ctccaataaa gtggaagctg tggtctggac ggtacctatc
    12721 ttaatcatca tcttccttgc ggtactgacc tggaaaacca ctcacgctct tgagcctagc
    12781 aagccgctgg cacacgacga gaagcccatt accatcgaag tggtttccat ggactggaaa
    12841 tggttcttca tctacccgga acagggcatt gcaaccgtga atgaaatcgc tttcccggcg
    12901 aacactccgg tgtacttcaa agtgacctcc aactccgtga tgaactcctt cttcattccg
    12961 cgtctgggta gccagattta tgccatggcc ggtatgcaga ctcgcctgca tctgatcgcc
    13021 aacgaacccg gtacttatga cggtatctcc gccagctaca gcgggccggg cttctcaggc
    13081 atgaagttca aagctattgc aacaccggat cgcgccgaat tcgaccaatg ggtcgcaaaa
    13141 gcgaaacagt cgccgaacac catgtctgac atggctgcgt tcgaaaaact ggccgcgcct
    13201 agcgaataca accaggtgga atatttctcc aacgtgaaac cagacttgtt tgccgatgtg
    13261 attaacaagt ttatggctca cggtaagagc atggacatga cccagccaga aggtgagcac
    13321 agtgcacacg aaggtatgga aggcatggac atgagccacg cggaatccgc ccattaaagg
    13381 ggttgaggaa gaataaagat gttcggaaaa ttatcacttg atgcagtccc gttccatgaa
    13441 cctatcgtca tggttacgat cgctggcatt attttgggag gtctggcgct cgttggcctg
    13501 atcacttact tcggtaagtg gacctacctg tggaaagagt ggctgacctc cgtcgaccat
    13561 aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga tgttgctgcg tggttttgct
    13621 gacgccatta tgatgcgtag ccagcaggct cttgcctcgg cgggcgaagc gggcttcctg
    13681 ccacctcacc actacgatca gatcttcacc gcgcacggcg tgattatgat cttcttcgtg
    13741 gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc cgctgcagat cggcgcgcgt
    13801 gacgttgcgt tcccgttcct caacaactta agcttctggt ttactgttgt tggtgtgatt
    13861 ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga ccggctggct ggcctatcca
    13921 ccgctatcgg gaatagagta cagtccggga gtcggtgtcg attactggat atggagtctc
    13981 cagctatccg gtataggtac gacgcttacc ggtatcaact tcttcgttac cattctgaag
    14041 atgcgcgcac cgggcatgac catgttcaag atgccagtat ttacctgggc atcactgtgc
    14101 gcgaacgtac tgattattgc ttccttccca attctgacgg ttaccgtcgc gttgttgacc
    14161 ctggatcgct atctgggcac ccatttcttt accaacgata tgggtggcaa catgatgatg
    14221 tacatcaacc tgatttgggc ctggggccac ccggaagttt acatcctgat cctgcctgtt
    14281 ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta aacgtctgtt tggttatacc
    14341 tcgctggtat gggcaaccgt ctgtatcacc gtgctgtcgt tcatcgtttg gctgcaccac
    14401 ttctttacga tgggtgcggg cgcgaacgta aacgccttct ttggtatcac cacaatgatt
    14461 atcgccatcc cgaccggggt gaagatcttc aactggctgt tcaccatgta tcagggccgc
    14521 atcgtgttcc attctgcgat gctgtggacc atcggtttta tcgtcacttt ctcggtgggc
    14581 gggatgactg gcgtgctgct ggcagtaccg ggcgcggact tcgttctgca taacagcctg
    14641 ttcctgattg cgcacttcca taacgttatc atcggcggcg tggtcttcgg ctgcttcgca
    14701 gggatgacct actggtggcc taaagcgttc ggtttcaaac tgaatgaaac ctggggtaaa
    14761 cgcgcgttct ggttctggat catcggcttc ttcgttgcct ttatgccgct gtatgcgctg
    14821 ggcttcatgg gcatgacccg tcgtttgagc cagcagattg acccgcagtt ccacaccatg
    14881 ctgatgattg cagctagcgg tgcagtactg attgcgctgg gtattctctg cctcgttatt
    14941 cagatgtacg tttctattcg cgaccgcgac cagaaccgtg acctgactgg cgacccgtgg
    15001 ggtggccgta cgctggagtg ggcaacctct tccccgcctc cgttctataa ctttgccgtt
    15061 gtgccgcacg ttcacgagcg tgatgcattc tgggaaatga aagagaaagg cgaagcgtat
    15121 aaaaagcctg accactatga agaaattcat atgccgaaaa acagcggtgc aggtatcgtc
    15181 attgcagctt tctccaccat cttcggtttc gccatgatct ggcatatctg gtggctggcg
    15241 attgttggct tcgcaggcat gatcatcacc tggatcgtga aaagcttcga cgaggacgtg
    15301 gattactacg tgccggtggc agaaatcgaa aaactggaaa accagcattt cgatgagatt
    15361 actaaggcag ggctgaaaaa tggcaactga tactttgacg cacgcgactg cccacgcgca
    15421 cgaacacggg caccacgatg caggcggaac caaaatcttc ggattttgga tctacctgat
    15481 gagcgactgc attctgttct caatcttgtt tgctacctat gccgttctgg tgaacggcac
    15541 cgcaggcggc ccgacaggta aggacatttt cgaactgccg ttcgttctgg ttgaaacttt
    15601 cttgctgttg ttcagctcca tcacctacgg catggcggct atcgccatgt acaaaaacaa
    15661 caaaagccag gttatctcct ggctggcgtt gacctggttg tttggtgccg gatttatcgg
    15721 gatggaaatc tacgaattcc atcacctgat tgttaacggc atgggtccgg atcgcagcgg
    15781 cttcctgtca gcgttctttg cgctggtcgg cacgcacggt ctgcacgtca cttccggtct
    15841 tatctggatg gcggtgctga tggtacaaat cgctcgtcgc ggcctgacca gcactaaccg
    15901 tacccgcatc atgtgcctga gcctgttctg gcacttcctg gatgtggttt ggatctgtgt
    15961 gttcactgtt gtttatctga tgggggcgat gtaatgagtc attctaccga tcacagcggc
    16021 gcgtcccatg gcagcgtaaa aacctacatg acaggcttta tcctgtcgat cattctgacg
    16081 gtgattccgt tctggatggt gatgacagga gctgcctctc cggccgtaat tctgggaaca
    16141 atcctggcaa tggcagtggt acaggttctg gtgcatctgg tgtgcttcct gcacatgaat
    16201 accaaatcag atgaaggctg gaacatgacg gcgtttgtct tcaccgtgct aatcatcgct
    16261 atcctggttg taggctccat ctggattatg tggaacctca actacaacat gatgatgcac
    16321 taagagcggc ggttatgatg tttaagcaat acctgcaagt aacgaaacca ggcatcatct
    16381 ttggcaacct gatctcggtg attgggggat tcctgctggc ctcaaagggc agcattgatt
    16441 atcccctgtt tatctacacg ctggttgggg tgtcactggt tgtggcgtcg ggttgtgtgt
    16501 ttaacaacta catcgacagg gatatcgaca gaaagatgga aaggacgaag aatcgggtgc
    16561 tggttaaagg cctgatctct cctgctgtct cgctggtgta cgccaccttg ctaggctttg
    16621 ctggctttat gctgctgtgg tttggcgcga atccgctggc ctgctggctg ggggtgatgg
    16681 gctttgtggt ttatgtcggc gtttatagcc tgtacatgaa acgccactcg gtctacggca
    16741 cattgattgg ttcgctctcc ggcgctgcgc cgccggtgat tggctactgt gcagtaaccg
    16801 gcgagttcga tagcggcgca gcgatcctgc tggctatctt cagcctgtgg cagatgcctc
    16861 actcctatgc catcgccatt ttccgcttta aggattatca ggcggcaaac attccggtat
    16921 tgccagtggt aaaaggcatt tcggtggcga agaatcacat cacgctgtat atcatcgcct
    16981 ttgccgttgc cacgctgatg ctctctcttg gcggttacgc tgggtataaa tatctggtgg
    17041 tcgccgcggc ggtgagcgtc tggtggttag gtatggccct gcgcggttat aaagttgctg
    17101 atgacaggat ctgggcgcgc aagctgttcg gcttctctat catcgccatc acagccctct
    17161 cggtgatgat gtccgttgat tttatggtgc cggactcgca tacgctgctg gctgctgttt
    17221 ggtaacacaa cctctctatt aaaaaggtgc tacggcacct tttttattgt ctgagatcag
    17281 agatacggct aataaccgta tgtcttgaca gagtgagcag atgaacataa attaatcata
    17341 cggttgatag ccgtattatg tatgagatcg actccctacg atttcattca gttaatcaaa
    17401 gaggtcataa tgcctggaaa aacagccact ctacccgatg tcgataaaac gctgaaaaat
    17461 gcgcgggtcg aactcaaaac tagtcccgac gccaaaaaca aacttcgtga agctgcgcaa
    17521 gccgttgggg tagatttaag cgcttttatt ttgagcgctg ctatggaacg cgccgaaagt
    17581 gtgcttgata accaacgtcg ccgtgagctt tcgaatcaaa gctgggaact gatgaaccaa
    17641 ctcatcgctg aacctgctca accgacgctc gccctcaagg cgttaatgaa aaggaaaaac
    17701 agcgatggtc gacaagcatg aagagattac tctgcccata gtcctctcct gtaattatca
    17761 gtctgatatt acttatcctg ggcaaaaaca gtttgattgc ggtaaccctg ttatcgataa
    17821 atttgtacgc gcatcgctaa agaaaagtgt gcgtaatagc gactgtgcgg ctaaagcact
    17881 tattgacaga caaagtggtg aactgatcgg catctgtact tttacggcat attcgctgga
    17941 aaaacaacgc gtttctggcg tccttcaggg ttcacaacct tcagaaattg gtgttgtcag
    18001 attagtcatg ttgggggtag cacggaagta tcaaaagcgg ggctttggtc aggacctact
    18061 atgtgatttt tttgaacatg taaaaataat tcaccaggca ttaccaatta aaggggttta
    18121 tcttgatgct gaccctgccg ccattaattt ttatgctcgt ctcggctttg ttcagctttc
    18181 agcgacacca aatgcttttg gtgctgtacc tatgtttttg gcgattcagc atattctcgc
    18241 ggcttagatt attatcgcca cccgccaggg caagttttgc cttagccggg ggctgagcag
    18301 cgttaccact tttgttcaat tgcccccgct ttcagcaact ccacatcctt gccgttaatg
    18361 gttgccgttg cggcaacgat tgtggtcctt acagttgcac aagattttcg ctcaaattcc
    18421 ggtttataaa gaataagaga cgaattactg gtaaatgaac caaataggta atcagtaaag
    18481 ctaatattca gccggtttaa accgaaaggc gcgaagccaa ccgtttggtt aatctcctta
    18541 ccggatgggc gacgaatgct gaatgtcacg cgaagattgg ccacttcgcc taatttattg
    18601 gtggtggcga agaggctatc catgcaggaa aaacctttat ctaaaaaatt atctcgcatc
    18661 ccagaacggg taaccgtaat cacgggtaac gtatcgtcaa ccgtttttct gtcaatcatg
    18721 tcttttcgac aagtagccag aaagtcttcc gggccaccat aaatgtggac acctttatca
    18781 agatattcct tcgctttctt acaatctacc tcaacatact gaccattggc gtacataatg
    18841 ccca
//
LOCUS       DAEEKQ010000072        18523 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.072, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000072 DAEEKQ010000000
VERSION     DAEEKQ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.072"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..1710
                     /locus_tag="KLK57_002375"
     CDS             <1..1710
                     /locus_tag="KLK57_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HBE0892726.1"
                     /translation="EMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQVQDSISTK
                     LSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLDALVDGPW
                     GGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGARFTWWGED
                     GNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPLLHRALVLAWAQ
                     EMNLPCKTLLKGLDEAIARRGTKDEDFSEEDIRDINDSLILDTPESPLADAVANVLTM
                     TLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAILSGLTAEQ
                     STIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVPDILSFGD
                     ELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSNEIDSDAL
                     YLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQLLEKARNE
                     MGELAYMVPVKELTGTVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAAGSEWTGM
                     QVK"
     gene            1711..3360
                     /locus_tag="KLK57_002376"
     CDS             1711..3360
                     /locus_tag="KLK57_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HBE0892727.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            3365..4141
                     /locus_tag="KLK57_002377"
     CDS             3365..4141
                     /locus_tag="KLK57_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HBE0892728.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTSGIGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="KLK57_002378"
     CDS             complement(4215..5399)
                     /gene="atoB"
                     /locus_tag="KLK57_002378"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA acetyltransferase"
                     /protein_id="HBE0892729.1"
                     /translation="MKNCVIVSAVRTAIGSFNGSLASTSAIDLGATVIKAAIERAKID
                     SLHIDEVIMGNVLQAGLGQNPARQALLKSGLAETVCGFTVNKVCGSGLKSVALAAQAI
                     QAGQAKSIVAGGMENMSLAPYLLDAKARSGYRLGDGQVYDVILRDGLMCATHGYHMGI
                     TAENVAKEYGITREMQDELALHSQRKAAAAIESGAFTAEIVPVNVVTRKKTFVFSQDE
                     FPKANSTAEALGALRPAFDKAGTVTAGNASGINDGAAALVIMEESAALAAGLTPLARI
                     KSYASGGVPPALMGMGPVPATQKALQLAGLQLADIDLIEANEAFAAQFLAVGKTLGFD
                     SEKVNVNGGAIALGHPIGASGARILVTLLHAMQARDKTLGLATLCIGGGQGIAMVIER
                     LN"
     gene            complement(5430..6752)
                     /locus_tag="KLK57_002379"
     CDS             complement(5430..6752)
                     /locus_tag="KLK57_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00366 family protein"
                     /protein_id="HBE0892730.1"
                     /translation="MIGRISRFMTRFVSRWLPDPLIFAMLLTLLTFVIALWLTPQTPI
                     SMVKMWGDGFWNLLAFGMQMALIIVTGHALASSAPVKSLLRTAASAAKTPVQGVMLVT
                     FFGSVACVINWGFGLVVGAMFAREVARRVPGSDYPLLIACAYIGFLTWGGGFSGSMPL
                     LAATPGNPVEHIAGLIPVGDTLFSGFNIFITVTLIVVMPFITRMMMPKPSDVVSIDPE
                     LLMEEADFQKQLPKDAPPSERLEESRILTLIIGALGIAYLAMYFSEHGFNITINTVNL
                     MFMIAGLLLHKTPMAYMRAISAAARSTAGILVQFPFYAGIQLMMEHSGLGGLITEFFI
                     NVANKDTFPVMTFFSSALINFAVPSGGGHWVIQGPFVIPAAQALGADLGKSVMAIAYG
                     EQWMNMEQPFWALPALAIAGLGVRDIMGYCITALLFSGVIFVIGLTLF"
     gene            complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="KLK57_002380"
     CDS             complement(6749..7399)
                     /gene="atoA"
                     /locus_tag="KLK57_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit beta"
                     /protein_id="HBE0892731.1"
                     /translation="MDAKQRIARRVAQELRDGDIVNLGIGLPTMVANYLPEGIHITLQ
                     SENGFLGLGPVTTAHPDLVNAGGQPCGVLPGAAMFDSAMSFALIRGGHIDACVLGGLQ
                     VDEEANLANWVVPGKMVPGMGGAMDLVTGSRKVIIAMEHCAKDGSAKILRSCTMPLTA
                     QHAVHMLVTELAVFRFIDGKMWLTEIADGCDLATVRAKTEARFEVAADLNTQRGDL"
     gene            complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="KLK57_002381"
     CDS             complement(7399..8061)
                     /gene="atoD"
                     /locus_tag="KLK57_002381"
                     /EC_number="2.8.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate CoA-transferase subunit alpha"
                     /protein_id="HBE0892732.1"
                     /translation="MKTKLMTLQDATGFFRDGMTIMVGGFMGIGTPSRLVEALLESGV
                     RDLTLIANDTAFVDTGIGPLIVNGRVRKVIASHIGTNPETGRRMISGEMDVVLVPQGT
                     LIEQIRCGGAGLGGFLTPTGVGTVVEEGKQTLTLDGKTWLLERPLRADLALIRAHRCD
                     TLGNLTYQLSARNFNPLIALAADITLVEPDELVETGELQPDHIVTPGAVIDHIIVSQE
                     SK"
     gene            complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="KLK57_002382"
     CDS             complement(8257..9642)
                     /gene="atoC"
                     /locus_tag="KLK57_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate metabolism transcriptional
                     regulator AtoC"
                     /protein_id="HBE0892733.1"
                     /translation="MTAINRILIVDDEDNVRRMLSTAFALQGFETHCANNGRTALHLF
                     ADIHPDVVLMDIRMPEMDGIKALKEMRSHETRTPVILMTAYAEVETAVEALRCGAFDY
                     VIKPFDLDELNLIVQRALQLQSMKKEIRHLHQALSASWQWGHILTNSPAMMDICKDTA
                     KIALSQASVLISGESGTGKELIARAIHYNSRRAKGPFIKVNCAALPESLLESELFGHE
                     KGAFTGAQTLRQGLFERANEGTLLLDEIGEMPLVLQAKLLRILQEREFERIGGHQTIK
                     VDIRIIAATNRDLQAMVKEGTFREDLFYRLNVIHLILPPLRDRREDISLLANHFLQKF
                     SSENQRDIIDIDPMAMSLLTAWSWPGNIRELSNVIERAVVMNSGPIIFSEDLPPQIRQ
                     PVCNAGEVKTASVGERNLKEEIKRVEKRIIMEVLEQQEGNRTRTALMLGISRRALMYK
                     LQEYGIDPADV"
     gene            complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="KLK57_002383"
     CDS             complement(9639..11465)
                     /gene="atoS"
                     /locus_tag="KLK57_002383"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     AtoS"
                     /protein_id="HBE0892734.1"
                     /translation="MHYMKWIYPRRLRNQMILMAILMVIVPTLTIGYIVETEGRSAVL
                     SEKEKKLSAVVNLLNQALGDRYDLYIDLPREERIRALNAELAPITENITHAFPGIGAG
                     YYNKMLDAIITYAPSALYQNNVGVTIAADHPGREVMRTNTPLVYSGRQVRGDILNSML
                     PIERNGEILGYIWANELTEDIRRQAWKMDVRIIIVLTADLLISLLLIVLFSRRLSANI
                     DIITDGLSTLAQNIPTRLPQLPGEMGQISQSVNNLAQALRETRTLNDLIIENAADGVI
                     AIDRQGDVTTMNPAAEVITGYQRHELVGQPYSMLFDNTQFYSPVLDTLEHGTEHVALE
                     ISFPGRDRTIELSVTTSRIHNTHGEMIGALVIFSDLTARKETQRRMAQAERLATLGEL
                     MAGVAHEVRNPLTAIRGYVQILRQQTSDPIHQEYLSVVLKEIDSINKVIQQLLEFSRP
                     RHSQWQQVSLNALVEETLVLVQTAGVQARVDFISELDNELSPINADRELLKQVLLNIL
                     INAVQAISARGKIRIQTWQYSDSQQAISIEDNGCGIDLSLQKKIFDPFFTTKASGTGL
                     GLALSQRIINAHQGDIRVASLPGYGATFTLILPINPQGNQTV"
     gene            11632..14481
                     /gene="rcsC"
                     /locus_tag="KLK57_002384"
     CDS             11632..14481
                     /gene="rcsC"
                     /locus_tag="KLK57_002384"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HBE0892735.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="KLK57_002385"
     CDS             complement(14681..15331)
                     /gene="rcsB"
                     /locus_tag="KLK57_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HBE0892736.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="KLK57_002386"
     CDS             complement(15348..18020)
                     /gene="rcsD"
                     /locus_tag="KLK57_002386"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HBE0892737.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEKIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAAGPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         4526 a         4535 c         4867 g         4595 t
ORIGIN      
        1 gagatgcgcc agacgctgac gctgcacaaa ggggcgaatt atatttcgct ggcgcagaac
       61 attcagcaat ctggcttgtt aagcgcagaa ctgcaacaaa atgggcaagt gcaggacagc
      121 attagcacaa aactgtcttt tgtggataac agctggcccg ttgaacagca gaaaaatgtc
      181 atgctcggcg gtggcgagaa cgcgctgatg ttgcccgagc aggctagcaa tatccggcta
      241 caaagtagcg aaacgccgca ggagattttc cgcaacaatc ttgatgcgtt agtcgatgga
      301 ccatggggtg gcgtgatcaa caccggtagc cgtctgatcc cgctcagtct cgcctggcgt
      361 tcgcttgccg atcatcaaag tgccgccgct aacgacattc gtcagatgat tcaggataac
      421 cgtctgcggc tgatgcaact ggcggggccc ggagcgcgct ttacctggtg gggtgaagat
      481 ggcaatggtg acgccttcct tacggcatgg gcatggtacg ccgactggca ggccagccag
      541 gcgctcggcg taacgcaaca accggaatac tggcagcata tgctcgacag ttatgccgag
      601 caggcggata acatgccgtt attgcatcgg gcgctggtgc tggcgtgggc acaggagatg
      661 aatctgccgt gcaaaacgtt gttgaaaggg ttggatgaag ctatcgcccg gcgcggaact
      721 aaagatgaag atttctctga ggaagacatc cgcgatatca atgacagcct gatcctcgat
      781 acaccggaat ctccactggc agatgcggtg gcaaacgtct taaccatgac gttgctgaaa
      841 aaagcgcagt tgaagtccac ggtgatgcca caggttcagc aatatgcgtg ggataaagcg
      901 gcaaacagca atcagccgct ggcgcacacg gttgtgctgc tcaatagcgg tggtgacgct
      961 acccagacgg ccgctatttt aagtggtttg accgctgagc aatccactat tgagcgcgcg
     1021 ctggccatga actggctggc gaaatatatg gcgacaatgc ctccagttgt tttgcctgcg
     1081 cctgcgggcg catgggctaa acataagtta actggagggg gcgaagactg gcgttgggtt
     1141 ggtcagggtg tgccggacat tctctctttt ggtgacgaat tatcgccgca aaatgtgcag
     1201 gtccgctggc gtgaaccggc aaaaacggct caacaaagta acattccggt gaccgttgaa
     1261 cgccagttgt atcggcttat ccccggtgaa gaagagatga gctttactct gcagccggtg
     1321 accagcaatg agattgacag cgatgcgctg tatctcgatg aaattacgct taccagcgag
     1381 caggatgcag ttctgcgcta tggtcaggtg gaagtaccgc tcccgccggg agccgacgtt
     1441 gagcgcacaa catggggcat ttcagtcaat aaacccaacg ccgcgaaaca gcaggggcaa
     1501 ttgctggaaa aagcgcgtaa tgaaatgggc gaactggcct atatggtgcc ggtgaaagaa
     1561 ctgacgggaa cggtcacttt ccgccatttg ctgcgcttct cgcaaaaagg gcaattcgtt
     1621 ctgcctcctg ctcgttatgt gcgttcctat gcacctgcgc agcaaagtgt tgcggcaggg
     1681 agtgaatgga ccgggatgca ggtgaaataa gtgaactggc gcagaatcgt ctggctattg
     1741 gcgttggtta ctctgccaac gcttgcggag gagacgccgt tacaactggt gctgcgcggt
     1801 gcgcagcacg atcaactgta tcagctgtcg tcatcaggcg tgaccaaagt ctcagcatta
     1861 cctgactcgc tcaccacgcc gttagggagt ttgtggaaac tgtatgttta cgcctggctg
     1921 gaggataccc atcaaccgga gcagccctac cagtgtcgcg gaaactcccc ggaagaggtt
     1981 tattgttgtc aggctgggga gagcataacg cgcgataccg cgctggtacg ttcctgtggg
     2041 ctctattttg cgccgcagcg gttgcatatt ggcgcagatg tgtgggggca atactggcaa
     2101 cagcgtcagg cacctgcgtg gttagcgtct cttacaacgc tgaagccaga aacctcggta
     2161 acggttaaat cgctgctcga ttcattagcg acgttacctg cgcaaaacaa agctcaggaa
     2221 gtcttactgg atgtggtgct ggacgaggcg aaaatcggtg tcgcatccat gctcggtagt
     2281 cgggtacggg tgaaaacctg gagctggttt gccgatgata aacaggaaat ccgccagggt
     2341 ggttttgctg gctggttaac cgatggtaca ccgctgtggg ttactggcag tggcaccagc
     2401 aaaacggtac taacccgtta tgcgacggtg cttaatcggg ttttgccagt gccgacgcag
     2461 gttgccagtg ggcaatgtgt agaggtggag ctgtttgccc gctatccgct gaagaaaatc
     2521 acagcggaaa agagtacgac ggcagtaaaa cccggcgtac ttaatgggcg ctatcgtgtg
     2581 acgtttacta atggtaacca cattacgttt gtcagccacg gtgagacgac cttactgagc
     2641 gaaaagggca aactgaaatt gcagtcgcat ctcgatcgtg aagagtacgt tgccagagtt
     2701 ctggatcgcg aggcgaaaag tacgccacct gaggcagcga aagccatgac cgtggcgata
     2761 cgtacttttt tacagcaaaa cgccaatcgg gaaggtgatt gcctgaccat ccccgacagc
     2821 agcgccacgc agcgtgtttc tgcttcgcca gcgaccacgg gggcgcgaac gatgaccgct
     2881 tggacgcagg atctcatcta tgcaggcgat ccggttcatt atcatggcag tcgcgctacc
     2941 gaagggacac tttcctggcg gcaagccacg gcccaggcgg ggcaggggga gcgttacgac
     3001 cagatactcg cgtttgctta tcccgacaac agccttagcc gctggggagc tccacgctca
     3061 acctgccagt tattacccaa agcgaaagcc tggctggcga aaaaaatgcc gcagtggcgg
     3121 cgtatattac aaggcgagac ggggtacaac gaaccagacg tgtttgcggt ctgtcgttta
     3181 gtctcaggtt tcccctatac cgatcggcag cagaaacggc tgtttattcg taacttcttt
     3241 acgcttcagg atcggctgga tttaacccat gagtatctgc acctggcctt tgatggttat
     3301 cccaccgggc ttgatgagaa ctatatcgaa acgctgaccc gacaattatt aatggactaa
     3361 tgctatgcga aagatttttc ttccgttgtt actggtggca ctttcgcctg tcgctcacag
     3421 tgaaggtgtg caggaagtcg agattgacgc accgctctct ggctggcatc cggcagaggg
     3481 cgaagatgcc agtttttcac aatcgatcaa ttacccggcg tcgtccgtca acatggcaga
     3541 tgatcaaaat atctcagcgc agatccgcgg caaaataaag aattatgctg cggcgggtaa
     3601 agttcagcag ggccggctgg tggtcaacgg tgccagtatg ccgcagagaa ttgaatccga
     3661 tggttcattt gcacgccctt atattttcac tgaaggcagc aacagcgtgc aggtcatcag
     3721 cccggatggg caaagccggc aaaaaatgca gttttactca acgtcgggta taggaacgat
     3781 tcgtgcacgt ttacggctgg ttctctcgtg ggatacggac aataccgatc tcgatctcca
     3841 tgttgtcacg cccgatggcg aacacgcctg gtacggtaac accgtgctga aaaacagtgg
     3901 tgcactggat atggatgtca cgacggggta cggacccgag attttcgcca tgccagcacc
     3961 agttcacggg cgctatcagg tgtatatcaa ctactatggt gggcgcagcg aaacggaatt
     4021 gacgaccgcc caactgacgc tgatcaccga tgaaggatcg gtcaatgaga aacaggaaac
     4081 atttattgta ccgatgcgta atgctggcga gctgacgctg gtgaaaagtt ttgactggtg
     4141 attaatgggg ttaccttcgc cgtcgatgtt caagaaaacg cccgataact ttcgctatcg
     4201 ggtgttttta ttgattaatt caaccgttca atcaccatcg caattccctg gccgccacca
     4261 atgcacagtg ttgccagccc cagcgtttta tcgcgtgcct gcatggcatg taatagtgtg
     4321 accagaatac gagcaccact ggcaccgata ggatgcccga gcgcgatggc cccgccgttg
     4381 acattcactt tctcagaatc aaagcccagg gttttcccaa cggcgaggaa ctgtgcagca
     4441 aatgcttcat tagcttcgat gagatcaatg tctgccagtt gcagccccgc cagttgtaac
     4501 gctttttgcg tggcaggtac tggccccata cccatcaatg cgggaggcac gccaccgctg
     4561 gcataacttt taatgcgagc caggggggta aggcctgctg ccagcgccgc agattcttcc
     4621 ataatcacca gagcggcagc accgtcgtta ataccagacg cgttcccagc ggtgactgtt
     4681 cctgctttat cgaaggccgg gcgcaatgca cctaacgctt cagccgttga attcgctttc
     4741 gggaattcgt cttgactgaa gacgaaggtt ttcttccgag tgacaacatt taccgggacg
     4801 atttcggctg taaaagcacc ggactcaatt gcggctgccg ctttacgctg tgaatgtagc
     4861 gccagttcat cctgcatttc acgggtaatt ccgtactctt tagccacgtt ttcggcggta
     4921 atccccatat gataaccatg ggtggcgcac atcaggccat cgcgcaggat tacgtcataa
     4981 acctgtccgt ctccaagacg ataaccagag cgtgcttttg catcgagtaa gtagggcgct
     5041 aaactcatat tttccatacc ccccgccaca atgctcttcg cctgacctgc ctgaattgcc
     5101 tgggcggcaa gcgccacact tttaagaccc gaaccacaca ctttattgac cgtgaatccg
     5161 cacaccgttt ctgccagccc gctttttaac agtgcctgac gtgccggatt ttgccccaac
     5221 ccggcttgca acacgttacc cataatcact tcatcaatgt gtagtgaatc gatttttgca
     5281 cgttcaatgg cggctttaat tactgtcgcc cccaggtcga tggcgctggt ggaagcgagt
     5341 gaaccgttaa aactaccgat agcagtacgt accgcactga cgatgacaca atttttcatt
     5401 ttatattcct tctgtctgta gggttgccgt cagaacagcg ttaaaccaat gacgaaaatg
     5461 acgccggaga agagcagggc agtgatgcaa tagcccatga tgtcgcggac accgagtccg
     5521 gcgattgcca gtgctggcag cgcccagaat ggttgttcca tgttcatcca ttgctcgccg
     5581 taggcgatcg ccattaccga tttaccgaga tcagcgccca gcgcctgggc tgcgggtatc
     5641 acgaaaggtc cctgaataac ccagtgaccg ccgccagacg gaacggcgaa gttaatcagt
     5701 gcagaactaa aaaaggtcat taccgggaag gtgtctttgt tcgcaacatt gatgaagaat
     5761 tcggtaatga gtccacccag accagaatgc tccatcatca gttggatccc agcgtagaag
     5821 gggaattgca ccagaatacc ggcagtactg cgtgctgccg cgctgatagc acgcatataa
     5881 gccattggcg ttttatgtag caacagaccc gcaatcataa acatcaggtt gacggtattg
     5941 atggtgatgt tgaagccatg ttcgctgaag tacatcgcca ggtaagcgat accgagtgcg
     6001 ccgatgatca acgtcagaat gcggctttct tccagtcgct cggatggtgg ggcatctttc
     6061 ggtagctgct tttgaaaatc agcctcttcc atgagtagtt ccggatcgat actcaccacg
     6121 tcagacggtt ttggcatcat catgcgggtg ataaatggca tcaccacaat caacgtcaca
     6181 gtgatgaaaa tgttaaaacc actgaacaga gtatcgccca ccgggatcag cccggcgata
     6241 tgctcaaccg ggttgcccgg tgttgcagcc aacagaggca ttgatccaga gaagccgcca
     6301 ccccaggtga gaaaaccaat gtaggcgcag gcaatgagca acggataatc agaaccgggg
     6361 actcgccggg cgacttcacg ggcaaacatt gcgccgacaa ccaaaccaaa tccccagttg
     6421 atgacacaag cgactgaacc gaagaaagtg accagcatga cgccctgtac gggcgtcttt
     6481 gcggcggagg cggcagtacg cagcaaactt ttcaccggag cagagctggc aagggcatga
     6541 ccggtaacga tgataagcgc catctgcata ccaaacgcca gcaagttcca gaaaccgtca
     6601 ccccacattt tcaccatgct gatcggcgtt tgtggtgtta accaaagcgc gatcacgaat
     6661 gttagcaatg tcagcaacat ggcaaagatc agtggatcgg gaagccatcg gctgacaaaa
     6721 cgcgtcataa aacgcgatat gcgaccaatc ataaatcacc ccgttgcgta ttcagatcgg
     6781 cggcgacttc aaaccgagct tctgttttgg cacgcacggt ggctaaatca cacccgtcgg
     6841 caatttcggt gagccacatt ttgccgtcaa taaaacgaaa gacagccagt tcagtaacca
     6901 gcatatgcac cgcatgttgc gcagtgagtg gcatggtgca gctgcgcaaa atttttgctg
     6961 aaccatcttt ggcgcaatgt tccatggcga tgatcacttt gcgcgacccg gtcaccagat
     7021 ccatcgcgcc acccataccg ggcaccattt tcccaggcac tacccagttc gcgaggtttg
     7081 cttcttcgtc tacttgcaaa ccgccgagca cgcaggcatc aatatgaccg ccacggatta
     7141 gcgcaaatga catggcgcta tcaaacatgg ctgcaccggg taaaacaccg cacggttgcc
     7201 cgccagcgtt caccagatct ggatgcgctg tcgtgaccgg gcctaaaccg aggaagccgt
     7261 tttccgattg cagagtgata tgaataccct ccggtaaata attggcgacc attgtgggta
     7321 aaccgatccc taagttaacg atgtcaccat cacgaagctc ttgcgccaca cggcgcgcaa
     7381 tacgttgttt cgcatccatt atttgctctc ctgtgaaacg atgatgtggt cgataacggc
     7441 accaggggtg acaatatggt caggttgcag ctcgccggtt tcgaccagtt catctggctc
     7501 taccagcgtg atatcagccg caagggctat cagggggtta aagttgcggg cgctaagttg
     7561 ataggtcagg ttgccaagtg tgtcgcaacg atgagcgcga attagcgcca ggtcggcgcg
     7621 cagtgggcgt tcgagcagcc aggttttacc gtcgagtgtc agtgtctgtt tgccttcctc
     7681 tacgacggtg ccgacacccg ttggggtgag aaaaccacca agtccagctc caccacagcg
     7741 aatttgctcg attagcgtac cttgcggcac cagaacgacg tccatctcac cagatatcat
     7801 gcgccgacct gtttccgggt tggtgccgat atgtgaagca atcactttgc ggactcgacc
     7861 attgacgatg agcggaccga tgccggtatc aacaaacgcg gtatcattgg ctatcaatgt
     7921 caggtcgcga acaccagatt ccagtaatgc ttcaaccagg cgggatggag tgccaatccc
     7981 cataaatccg cccaccatga tggtcatgcc gtcacgaaag aagccggtgg cgtcttgtaa
     8041 tgtcatcaat tttgttttca ttttttatcc ctcttgcata caccgttgtg ggtacagtca
     8101 ggcaatagca aggggagtgc cagaatgaga ttattggatt tagttattta actaattgaa
     8161 tatgaaggga taaattggat ggggatttat aaggagaatg agcggctatg cagaaaattg
     8221 cgcactgtgc aaatttctgc atagcaagtt ttggtgttat acatccgccg gatcgatacc
     8281 gtattcctgg agtttataca tcaatgcacg gcgactgatg cccagcatta aagcagtgcg
     8341 ggttcggttt ccttcttgtt gttccagcac ttccataatg atgcgttttt cgacgcgttt
     8401 aatttcctct tttaaattac gctcaccgac agaggctgtt tttacctcgc cagcattaca
     8461 gactggctga cgaatctgtg gcggaagatc ctcagaaaaa atgatcgggc ctgaattcat
     8521 cacgacggcg cgttcaataa cgttagaaag ctctcgaata tttcccggcc atgaccaggc
     8581 ggtaagcagt gacattgcca tcggatcgat gtcgataata tcgcgctgat tctcactact
     8641 gaatttttgc aaaaagtgat tagctaacag ggaaatatct tcccggcgat cgcgcagagg
     8701 cggcagtatt aaatgaataa cgttaaggcg ataaaagaga tcttcacgga aggtgccttc
     8761 ttttaccatt gcctgcaagt cacggttggt ggcagcaatg atgcggatat caacttttat
     8821 ggtctgatgg ccgccaatcc gttcaaattc ccgttcctgt agaatgcgta gtaatttggc
     8881 ttgtagtacc agcggcattt cgccaatttc gtcgaggagc agagtacctt cgttggctcg
     8941 ttcaaataat ccctgacgca aggtttgtgc accagtaaat gcaccttttt catgaccaaa
     9001 cagttcactt tcgagcaacg attccggcag cgccgcgcag ttgactttaa tgaacggccc
     9061 ctttgcccgc cgcgaattgt agtgaatcgc tctggcaatc aactctttcc cggtgccgct
     9121 ttcaccgcta atcaagacgc tggcctgaga aagggcaatt ttggcggtgt ctttgcagat
     9181 gtccatcatc gccgggctgt tggtgagaat gtgcccccat tgccagctgg cgctcagtgc
     9241 ctggtgcaga tgacggatct cttttttcat tgactggagt tgtaaagcgc gctgaacgat
     9301 taaattcaac tcatcgagat caaacggttt aataacatag tcgaaggctc cgcagcgtag
     9361 cgcttcgacg gcggtttcca cttccgcata ggccgtcatc agaataacgg gtgtccgggt
     9421 ctcatggctg cgcatctcct ttagtgcctt gatgccgtcc atctctggca tgcggatatc
     9481 catcaacacc acatcagggt gaatatcggc aaacaggtgt aatgctgtgc gtccgttgtt
     9541 cgcacaatgt gtttcgaatc cttgtagtgc aaaagcggtg ctcagcatac ggcgaacatt
     9601 atcttcatca tccacaataa ggatgcgatt aatagcagtc atacagtctg atttccctgc
     9661 gggttgatcg gtaaaataag cgtgaaggtt gcgccgtagc ccggcaaact ggcgacgcga
     9721 atatcaccct gatgggcatt aatgatgcgt tgacttaacg ccagaccaag cccggttcct
     9781 gaggctttgg tggtgaaaaa gggatcgaag atcttttttt gcagcgagag atcaatgcca
     9841 cagccgttgt cctctatcga aatggcctgt tgtgagtcgc tgtattgcca ggtttgaatg
     9901 cgaattttcc ctcgtgcgct gatagcctgg acggcattga tcaggatatt cagtagtacc
     9961 tgtttgagca gttcacgatc ggcgttaatc gggcttaatt cattatccag ttcgcttatg
    10021 aagtcgaccc gcgcttgtac gccggcggtt tgtaccagta ccagagtttc ttcaaccaat
    10081 gcattgaggc tgacttgttg ccattgactg tggcgtggac gtgaaaattc gagcaattgc
    10141 tgaataactt tgttaattga atcgatttct ttgagtacta cggacagata ttcctgatgt
    10201 attgggtcac tggtttgttg gcgcaagatc tgtacataac cacgaatagc cgttaacgga
    10261 ttacgtactt catgcgcgac gccagccatc agctcaccca gtgtggcgag gcgttctgct
    10321 tgcgccatgc ggcgctgggt ttctttgcgg gcagttaaat cagagaaaat caccaaagca
    10381 cctatcattt caccgtgcgt gttatgaata cgactggtag tgacactgag ttcaatggtg
    10441 cggtcacgac ctggaaaact gatctccagc gccacatgtt cggtgccatg ttccagcgta
    10501 tccagtactg gactgtagaa ctgagtattg tcgaacaaca tggagtaagg ctgccctacc
    10561 agttcatggc gttgatagcc agtgataact tctgctgctg ggttcatggt ggttacatca
    10621 ccctggcggt caatggcaat gacgccatcg gcagcgtttt caataatcag atcgttaagt
    10681 gtccgcgttt cacgcagtgc ctgggcgagg ttattaacac tctgactgat ttgccccatt
    10741 tcaccgggca attgtggtaa tcgagtggga atattttgtg ccagagtcga gaggccatcg
    10801 gtgatgatat caatattggc gctcaggcga cgggagaaaa ggacaatcaa tagcaggctt
    10861 atcagcaaat cggcggtgag cacaatgata atcctcacat ccattttcca ggcctggcgg
    10921 cgaatatctt cggttaattc attggcccag atatagccga ggatttcacc attacgctca
    10981 atggggagca ttgaattcaa aatatcgccg cgcacctgcc tgcctgaata aaccaaaggg
    11041 gtatttgtac gcatgacttc gcgaccaggg tgatctgcgg caatggtaac gccgacatta
    11101 ttctgatata gcgctgaagg cgcgtaggtg attatcgcat ccagcatttt gttgtaataa
    11161 ccagcaccta tgccagggaa ggcgtgagtg atattttcgg taatgggggc aagttctgca
    11221 tttaatgcgc ggatacgctc ctcacgtggt aagtcgatgt agagatcata gcgatcgcct
    11281 agtgcctgat taagcaggtt gaccacggca gaaagttttt tctctttttc agataagact
    11341 gctgaacgtc cttccgtttc tacgatataa ccaatagtaa gcgttgggac aatgaccatc
    11401 aggattgcca tcaggatcat ttgattgcgt aagcggcgtg gataaatcca cttcatataa
    11461 tgcatgctga aatccactag tcttgtccgg tatatgacga ttatcagagg ttaaggtgat
    11521 gatttctcgg cggtgtatca tattccagag aagagagaac attgcggtaa cacgctttta
    11581 ccgctacctt aaccacactc catcggtcac ctgaggcgga gcttcgcccc tttgaaatac
    11641 cttgcttctt ttcgtacaac cctgaaagcc tcgcgctaca tgttcagagc attggcgtta
    11701 gtgctctggc tgttgattgc tttttcatcc gttttttaca tcgttaatgc gttacatcag
    11761 cgagaatcgg aaattcgtca ggaatttaat ctgagttccg atcaggctca gcgctttatt
    11821 caacgcacct ctgatgtgat gaaagagctg aagtacatcg ccgaaaatcg cttatcggca
    11881 gaaaacggtg tgctttcccc gcgtggacga gaaacgcagg cggatgtgcc tgcgtttgaa
    11941 ccgctgtttg ccgactccga ttgttccgca atgagtaaca cctggcgagg ttctctggag
    12001 tcattggcgt ggtttatgcg ctactggcgc gataattttt ctgcggctta cgatctaaac
    12061 cgggtatttt taatcggcag cgataacctc tgcatggcca atttcggtct gcgtgatatg
    12121 ccagtggaac gcgataccgc gttgaaagct ttgcatgaac gcatcaataa atatcgaaat
    12181 gcaccacaag atgatagcgg cagtaacctc tactggatca gcgaaggtcc gcgccctggc
    12241 gtcgggtatt tttacgcgtt gacgccagtt tatctggcga accggttgca ggcgcttttg
    12301 ggtgtcgagc agaccatccg gatggagaac tttttcttac cgggtacgtt gccgatgggg
    12361 gttaccattc ttgatgaaaa tggtcatacc ctgatttcgc ttaccggacc agaaagtaaa
    12421 attaagggcg atcctcgctg gatgcaggaa cgctcctggt ttggctatac ggaagggttc
    12481 cgggagctgg tgctgaagaa aaatctgcca ccctcatcgc taagcatcgt gtattcggtg
    12541 ccggttgata aggtgctgga acgcattcgc atgttgatcc ttaacgcaat tttgctgaat
    12601 gtgcttgccg gagctgcatt gtttactctc gcacggatgt acgagcgacg tattttcatt
    12661 ccggcggaaa gcgacgccct gcgactggaa gaacatgagc agttcaatcg caagattgtc
    12721 gcctccgcgc cagtgggtat ctgcattttg cgtaccgctg atggcgtcaa tattttaagt
    12781 aacgaactgg cgcataccta tctcaatatg cttacgcatg aggaccgcca acgactgacg
    12841 caaattatct gtgggcagca ggtcaatttt gttgatgtcc tgaccagcaa caataccaat
    12901 ctgcaaatca gcttcgtcca ttcgcgctat cgtaatgaaa acgtggccat ttgtgtgctg
    12961 gtggatgttt cttcgcgcgt gaagatggaa gagtcgttgc aggagatggc acaagcagcg
    13021 gaacaggcga gccagtcaaa atcgatgttc cttgccaccg tcagtcatga gctgcgaacg
    13081 ccgctgtatg gcattatcgg taacctggat ctgttgcaaa ccaaagagtt accgaaaggc
    13141 gtcgatcggc tggtgacggc aatgaacaac tcttccagcc tgttgttgaa aattatcagc
    13201 gatattctcg atttctcgaa gattgaatcg gaacagttga agatcgaacc gcgtgagttt
    13261 tcaccgcgtg aagtgatgaa ccacatcacc gccaactatt taccgctggt ggtacgcaag
    13321 cagttaggct tgtactgctt tattgaaccg gatgtgccag tggccttaaa tggcgacccg
    13381 atgcgtttac agcaggtcat ctccaacctg ttgagtaacg ccataaaatt caccgatacc
    13441 ggctgtatag ttttgcatgt ccgcgctgat ggcgattatc tctctatccg tgttcgcgat
    13501 accggcgtgg ggattccggc gaaagaagtg gtgcgcttgt ttgatccctt cttccaggtc
    13561 ggaacgggcg tacagcgaaa cttccagggg accggtctgg gtctggcgat ttgtgaaaaa
    13621 ctgatcagca tgatggacgg cgatatctcg gtagattcag aaccgggaat gggcagccag
    13681 tttaccgtgc gtattccgtt gtacggcgct cagtacccgc agaaaaaagg cgtggaaggg
    13741 ttgagtggta aacgctgctg gctggcggtc cgcaatgcgt cgctctgtca gttcctggaa
    13801 accagtttgc agcgcagcgg catcgtcgtt acaacatacg aagggcagga accgactccc
    13861 gaagatgtgt taattactga cgaggtagtg agtaaaaaat ggcagggtag agcggtagtg
    13921 accttctgtc gtcgccatat tggtattccg ctggagaaag cgccagggga gtgggtacac
    13981 agtgtggctg ccccgcatga gctaccggca ttgttggcgc gtatttattt gatcgagatg
    14041 gagagcgacg atcctgctaa cgctctgccg tcgacggaca aagcggtcag cgataatgac
    14101 gatatgatga ttctggtcgt ggatgatcat ccgattaacc gacgtctgct ggcagatcag
    14161 ttgggatcgt tgggctatca atgtaaaacc gcgaatgatg gtgtcgatgc gcttaatgta
    14221 cttagcaaga atcatattga tatcgtgctt agcgacgtca acatgccaaa tatggatggt
    14281 taccgcttga cgcaacgcat tcgtcagttg ggactgacgt tgccggtaat cggagtaact
    14341 gctaatgcgt tggctgaaga gaagcagcgg tgtctggagt ccggtatgga cagctgcctg
    14401 tcgaagccgg taacgctgga tgtgataaaa cagacgctga cggtatatgc cgagagggtc
    14461 aggaaatcgc gggaatcgta ggacggataa ggtgtttatg ccgcatctgg cattcagtgc
    14521 aaatgccaga tgcgacgctg acgcgtctta tctggcctac tttgatgtaa tctgttgaaa
    14581 taatgggaat cgtaggacgc ataaggcgtt tacgccgcat tcggcaatcg gtgcaaatgc
    14641 cagataagac actaacgcgt cttatctggc ctacaggtga ttagtcttta tctgccggac
    14701 ttaacgtcac tgaagagaga taattcagca gggcgatatc gttctcgaca cccagcttca
    14761 tcatcgcaga tttcttctgg ctactgatgg ttttaatact gcggttcagc tttttagcga
    14821 tctcggtcac caggaagcct tccgcaaaca ggcgcagaac ttcactctct tttggcgaga
    14881 gacgcttgtc accgtaacca ccagcactga ttttttccaa caggcgagaa acgctttccg
    14941 gggtaaattt cttccctttc tgcagcgcgg cgagagcttt cggcagatcg gtcggtgcac
    15001 cttgtttcag cacgatccct tcgatatcca gatccaatac cgcactaaga atcgccgggt
    15061 tgttgttcat agtcagaaca atgatcgaca ggcttgggaa atggcgcttg atgtacttga
    15121 ttaaggtaat gccatcgccg tacttatcgc caggcatgga gagatcggta atcaacacat
    15181 gcgcatccag tttcggcagg ttgttgatca gtgctgtaga gtcttcaaat tcgccgacaa
    15241 cattcaccca ctcaatttgc tcaagtgatt tgcgaatacc gaacaagact atcggatggt
    15301 catcggcaat aattacgttc atattgttca tgtaataggc taccttgcta cagcaagctc
    15361 ttgacataac tgtcaatgtc gctgatgtat ttttctattc ctggaacatc cttctcacga
    15421 atcagatgtt ccagcgtttc acataactgc ttgccgggta ccagatttag catggcaaat
    15481 acgcctttaa gacgatgagc cgtttgggct aacgcagcaa agtcactggt tgctgcttca
    15541 gtatacagcc tcttaacatc atccggtact gtgtctacaa agagcgcata atagccgctg
    15601 gcatggagtt gcgcattttc atctccgccc agaggcgatt ctgtcacctc ttcctgcgcc
    15661 agttgcactt caattaattg taagaccgct tcctgcatag cgttgctcat attgaagttg
    15721 acgcacaatt gaccaggccc aatttcccgt acgccagact catcatcgct taaaagcaag
    15781 ccagaggcag taagattaga cggattatcc gttaaaaaga tatcataatc ttgactaatt
    15841 aatctttcat cgggtgtgat acaggttgca ccccaatttt ctaactggcg agtgacaata
    15901 ttccgaattt ctgccgaagt aacatccacc attacgcaga catcatccag taaacgctct
    15961 tcttcctctt caacttccgg gccagcggcg agcattttga tatgcacaga gtagcgtgta
    16021 ccaagcccat cccgcgtttt gatgtttaaa tgaccgccca gtttacgtgc cagttgatcg
    16081 ctcagccaga atgccagcgg gtccgccttt ccataacgat cgttttgggt ctggttgata
    16141 aacgggaagt gcaaattatc catttcatga atacttacgc cttctccggt gtccagaatg
    16201 cggaatgtca ggcggtcttc agaggactca tcctgatcaa cctcaagggt gatttttccc
    16261 aattgcgttg aggtcacggc atattgcatc agtagcagca aaatacgtcg taaggcatcg
    16321 cgatcgccgc ggcgcatatc gtgtgctttc agatgattgt taatcagcag ttgcagacct
    16381 ttacgcttga tggcaggcaa cactgaaggc acaacttcat caattaaatc ctgcacggag
    16441 aacagcaccg tctcactttt ccagctatcg tccgcaagca tgttcgctaa ctgtatttca
    16501 tcgaccagcc gcaccagcac atctgcctga ttcgccagtt gtttgctttc cggggcgttg
    16561 agtttagccg cgctctccgc cagggactgt gcaggttctt tcagcgcatc gccaatgttt
    16621 ttcataaagg tcatccggcc ctgctggttt ttctcataca gacgctgcgc ctgcttgagt
    16681 ttcttgttta ccagcacttc gcgatcctga tcgcgaataa tgaaaatttg tgtgcgcggc
    16741 gcgacctggc tgcggaacat gcggatctca tacagctcgt tattgatcgt cgcctgaata
    16801 atcccctgat gctgttccgc catggtggtg atgttttgca gattcaaatg cggcagcaaa
    16861 tgatcggcaa ttttgttact tatgacagtg cggttcgatt cctgatcgtg aaccagcagg
    16921 ccgagcggca gcagtgagac tatcttttca ttgattgccc gtaaaatgcg caattcgtta
    16981 ttgaccgccg tgttgggtag actttctgta ctgcgactgg agaaatggcg gaatgtggta
    17041 tagccaaata acgccagcgc cagcaaaccg atgttcagca gcagtggcag cagaatgttt
    17101 tgcaacgtat ccagcaataa ggtgccataa ggaacctgcc agaccaggcg catatcggta
    17161 gagttgagtg ccgaggagat ttcaatcttc gtactgttaa agtggatact gacgctatcc
    17221 gttccttctt tctcattatc attgtctccc gttgccgtcg tgtctggctc aaggcggaaa
    17281 ctgtccagcg gcatacccgg tgggatcaaa tcattaatcg gcagatcaaa agccacgacc
    17341 gttgccagat gtcccggctg gttgaacgtg gtacgcaagg taaagtaatg accgttctgc
    17401 caggccaggc ggcgcatgtt agaaaaactt tcgcgttcat cgagggcgtt ggcctgttgc
    17461 aacatttctg cacgacgtga atcaacaatg tcactgacgg tcgattcttt aaatccggag
    17521 gtgagatctt tgaggggcag ggttgagatc agtaccagac tgttatcctg accattcagg
    17581 taatacatcg accacggtac attttctgca ccccacaatg tatccagata agtggacatc
    17641 cgctgagtca tctcaagcgt tgagttgtcg tgagagccaa agattaacgc ttccgttttg
    17701 cggcgtggtt tctccagata gtagacatcc tgtttcaggc gcgtctcttg taaaccttcg
    17761 ccggaggagg gggaggtcgt cgcggcaatg ttgtcgtaga tctgccaggt cacgtaacgc
    17821 caggtatcga cgcgcttttg gattgcgtgg gtaatgtcga caatctggta acttttatct
    17881 ttcagccagg cgttaacggc gctttgtacc attaccccca tcgtcaccag taacacaatg
    17941 atcaacagta aaaagaagcg ggtaatgctc cccggtagga gtgaaaagcg ggtcgtggcc
    18001 gttgtctctt tctgacgcat tcgtgtttat gacctgttaa aacttcgcga agtgataggg
    18061 cagtataaag ggtacagtgt gaaattccag actcttacct gattgatgat tctctattct
    18121 cccgtgatat ttatcatcct gcattggggg aaatagatgt acaagcgcca ttttgctgta
    18181 caaataacct acaaaaagcc caacgaaatt aattgcaaat aaacgaagat tgctggaaat
    18241 tatgctgatg ttaattattt gtgaaatagt tgacaagagt tatagttttt ctgtggtagc
    18301 acagaataat gaaagtgtgt aaagaagggt aaaaaaaacc ggatgcgagg catccggttg
    18361 aaataggggt aaacagacat tcagaaatga atgacggtaa taaataaagt taatgatgat
    18421 agcgggagat attctagttg cgagtgaagg ttttgttttg acattcagtg ctgtcaaata
    18481 cttaagaata agttattgat tttaaccttg aattattatt gct
//
LOCUS       DAEEKQ010000073        18515 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.073, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000073 DAEEKQ010000000
VERSION     DAEEKQ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.073"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            78..290
                     /gene="cspH"
                     /locus_tag="KLK57_002387"
     CDS             78..290
                     /gene="cspH"
                     /locus_tag="KLK57_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HBE0892738.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            737..1042
                     /locus_tag="KLK57_002388"
     CDS             737..1042
                     /locus_tag="KLK57_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892739.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAASEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            1149..1793
                     /gene="gfcB"
                     /locus_tag="KLK57_002389"
     CDS             1149..1793
                     /gene="gfcB"
                     /locus_tag="KLK57_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HBE0892740.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            1790..2536
                     /locus_tag="KLK57_002390"
     CDS             1790..2536
                     /locus_tag="KLK57_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBE0892741.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPMEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            2536..4632
                     /locus_tag="KLK57_002391"
     CDS             2536..4632
                     /locus_tag="KLK57_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBE0892742.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            4678..5817
                     /locus_tag="KLK57_002392"
     CDS             4678..5817
                     /locus_tag="KLK57_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HBE0892743.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            5805..6251
                     /gene="etp"
                     /locus_tag="KLK57_002393"
                     /gene_synonym="yccY"
     CDS             5805..6251
                     /gene="etp"
                     /locus_tag="KLK57_002393"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HBE0892744.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     IKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            6271..8451
                     /gene="etk"
                     /locus_tag="KLK57_002394"
     CDS             6271..8451
                     /gene="etk"
                     /locus_tag="KLK57_002394"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HBE0892745.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(8566..9864)
                     /gene="appA"
                     /locus_tag="KLK57_002395"
     CDS             complement(8566..9864)
                     /gene="appA"
                     /locus_tag="KLK57_002395"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HBE0892746.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIKTALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="KLK57_002396"
     CDS             complement(9944..10036)
                     /gene="cbdX"
                     /locus_tag="KLK57_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HBE0892747.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            complement(10049..11185)
                     /gene="appB"
                     /locus_tag="KLK57_002397"
     CDS             complement(10049..11185)
                     /gene="appB"
                     /locus_tag="KLK57_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HBE0892748.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            complement(11197..12741)
                     /gene="appC"
                     /locus_tag="KLK57_002398"
     CDS             complement(11197..12741)
                     /gene="appC"
                     /locus_tag="KLK57_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HBE0892749.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVIFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKTVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLPLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            complement(12875..13732)
                     /locus_tag="KLK57_002399"
     CDS             complement(12875..13732)
                     /locus_tag="KLK57_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HBE0892750.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPISCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSQRQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRKRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="KLK57_002400"
     CDS             complement(13789..14127)
                     /gene="hyaE"
                     /locus_tag="KLK57_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HBE0892751.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSEFRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLREFPGYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHP"
     gene            complement(14124..14711)
                     /locus_tag="KLK57_002401"
     CDS             complement(14124..14711)
                     /locus_tag="KLK57_002401"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HBE0892752.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTLEEQG"
     gene            complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="KLK57_002402"
                     /pseudo
     CDS             complement(14708..15414)
                     /gene="hyaC"
                     /locus_tag="KLK57_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
     gene            complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="KLK57_002403"
     CDS             complement(15433..17226)
                     /gene="hyaB"
                     /locus_tag="KLK57_002403"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HBE0892753.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFNKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNTSPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            complement(17223..18341)
                     /gene="hyaA"
                     /locus_tag="KLK57_002404"
     CDS             complement(17223..18341)
                     /gene="hyaA"
                     /locus_tag="KLK57_002404"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HBE0892754.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAAWPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDCGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
BASE COUNT         4545 a         4913 c         4783 g         4274 t
ORIGIN      
        1 cggcggtctt aaatatcgct ggctttattt tattaacccc gtatagtgca gcgcagtatt
       61 ttcagtaagg aatttgtttg tcccgtaaaa tgacaggaat tgtcaaaacc tttgatcgca
      121 aaagcggcaa aggattcatt atcccctccg acggtcgtaa agaagttcag gtccatattt
      181 ccgcattcac tccccgcgac gcagaagtgc ttataccagg attacgtgtg gaattttgcc
      241 gcgtaaatgg cctgcgagga ccaacagcgg caaatgttta tctctcttaa tatgacgctg
      301 cttgtttaaa gctgactggt tttattggaa tagtgcaata gtcaacatca tacattataa
      361 tatcagttac ttttccacaa agaaactaat ccatttaagc gaatatctct gcacctaata
      421 gttataaatg ccatttaaca gccagttata gtaccacttg aaactatatt accaacaata
      481 attaatagct ttcgtaacaa tttccttatc tttaatttgt attttgctaa gatttatctt
      541 atgctcacaa gtcgtatttc cagaggggat cgttaataat cttatatttc cctctgcgca
      601 cctttcctac ccgcagtatc agtatatagt gcaagcggta tgaggaaagg gagatttcac
      661 aaggatgtta tactcaaagt agacaatcct gatctcaagc gtacgtattg tccatgcgat
      721 gaataaagga aaagttatga aacacaagct ttctgcaatc cttatggcct tcatgttgac
      781 gacgccagct gcgtttgctg cctctgaagc aacaaacggg accgaggcaa cgacgggtac
      841 tactggcacc acaacgacca ctaccggcgc aaccacgact gctactacca ctggtggcgt
      901 ggctgctggc gctgtcggca cggcgacagt tgtcggtgtg gcaacggctg ttggtgtggc
      961 gacgttggca gttgttgcag caaatgacag tggcgatggc ggttctcata acacctcaac
     1021 caccacgtcc actacacgct aatactgctt taacgcgtcg aaattaaagt taaatgatac
     1081 ccggttgcga tattcgtacc gggctttaga gatttgcatt tttttaatta acctttcaag
     1141 gattaaaggt gcgccctctt attttatcga ttttcgcact atttcttgcg ggatgtacgc
     1201 acagccagca aagtatggtc gatacatttc gcgccagcct tttcgataat caggatatca
     1261 ccgtagcgga tcagcagatc caggcgttgc cttattccac gatgtattta cgccttaatg
     1321 aagggcaacg aatctttgtg gtactgggat atatagaaca agaacaaagc aaatggttat
     1381 cccaggataa cgccatgctg gttacccaca atggacgtct tttaaaaacc gtcaaactta
     1441 ataataatct gctggaagtg acaaattccg ggcaggaccc tctgcgtaac gcgctggcaa
     1501 taaaagatgg cagccgctgg acgcgcgata ttctctggag tgaagacaac cattttcgct
     1561 ctgcgaccct gagttctact ttttcctttg ctggattaga gacgctgaat attgcgggtc
     1621 gcaatgtgct gtgtaatgtc tggcaggaag aggtgacttc cacgcggcca gaaaaacagt
     1681 ggcaaaacac attctgggtc gattcggcta ctggccaggt tcgtcaaagt cggcaaatgt
     1741 taggcgcagg ggttattccc gtagaaatga cgtttcttaa acccgcacca tgaataaatt
     1801 acagtcgtat ttcattgcca gcgtacttta cgtaatgaca ccccatgcct ttgcgcaagg
     1861 aacggtgact atttatctgc ctggcgaaca acagacactt tccgttggcc ccatggaaaa
     1921 tgttgtccag ttggtgacac aaccgcaact gcgcgatcgc ctctggtggc ctggtgcctt
     1981 gctgaccgac tctgcggcga aagctaaagc gcttaaagac taccaacatg tcatggcaca
     2041 gcttgcttcc tgggaagcag aagctgacga cgatgtggcg gcaacgatta aatccgtgcg
     2101 ccagcagtta ctcaatctca atatcactgg tcgcttaccg gtcaaactgg accctgattt
     2161 tgtacgagtg gatgagaaca gcaatccccc gttggtcggg gactatacgc tctataccgt
     2221 acagcgtccg gtgactatta ctctgctggg tgcggtttct ggggccgggc aattgccctg
     2281 gcaggcaggc cgtagcgtta ccgattatct gcaagatcac ccccgccttg caggcgcgga
     2341 caagaataac gttatggtga ttacgcctga gggtgagact gtcgtcgccc ctgttgccct
     2401 gtggaataaa agacatgtgg aaccaccgcc tggcagccag ctttggttgg gcttttctgc
     2461 gcatgtactg cccgaaaaat atgctgattt aaatgaccag attgtgtctg ttttaacgca
     2521 acgtgttcct gactgatgaa gaagaattct tatcttttaa gctgcctggc cattgccgtc
     2581 tccagtgcct gtcatgctga agtattaacc tacccggatc cgctgggttc gtcgcaatca
     2641 gactttggcg gcacaggatt gttgcagatg ccaaatgcgc gcatcgcacc ggaaggtgaa
     2701 ttcagcgtca actaccggga taacgatcaa taccggttct actccacctc cgtggcgctg
     2761 ttcccatggc tggaaggcac cattcgttat acggatgtgc gcacacgcaa atatagccag
     2821 tgggaagatt tcagcggcga tcagtcatac aaagacaaat cattcgattt taaacttcgc
     2881 ctgtgggaag aaggttactg gctaccgcaa gtggcgtttg gtaaacgtga tattgctggt
     2941 acgggtctgt ttgacggtga gtatctggtg gccagcaagc aagcggggcc atttgatttc
     3001 accctcggga tggcatgggg ctacgccggt aatgcgggca atattaccaa cccgttttgc
     3061 cgggtgagcg ataaatattg tcatcgcgca gagtctcacg atgcgggcga tatcagcttt
     3121 agcgatatct ttcgtggccc ggcttccatc tttggcggca ttgagtatca aacgccgtgg
     3181 aatcccctgc gtctgaaact cgaatacgat ggcaacaatt accagaatga tttcgctggc
     3241 aaactgcctc aggcaagcca tttcaacgtc ggcgcagttt atcgcgctgc cagctgggca
     3301 gatctcaacc tgagttatga acgcggtaac acgttgatgt ttggcttcac gttacggacc
     3361 aatttcaacg atctgcgccc tgccctgcgc gatacgccaa aaccggcata tcaacctgcg
     3421 cctgaatctg aaggattgca gtacaccaca gtagcgaacc aacttaccgc gctgaagtac
     3481 aacgcaggtt ttgaagcacc ggaaattcag ctgcgcgata agacgctgta tatgtctggt
     3541 caacaataca aataccgtga ttctcgcgaa gcggtcgatc gtgccaaccg gattctggtg
     3601 aataacctgc cgcaaggcgt tgagaagatt agcgtgacgc aaaagcgcga gcatatggcg
     3661 atggtgacta ccgaaaccga cgtagccagc ctgcgcaaac agctggctgg tacagcgcct
     3721 ggtcaatcag agcaacttca acaacaacgt gttgaagcag aagatctttc tgcctttggt
     3781 cggggctacc gtattcgtga agatcgcttt agctactctt tcaacccaac actttcacag
     3841 tcgctgggcg gcccggaaga tttctatatg ttccagctgg ggctgatgtc cagtgcccgc
     3901 tactggttta ccgaccacct gctgcttgat ggcggtattt tcaccaatat ttacaacaac
     3961 tacgacaagt ttaagtcttc gctgttgccc gcggactcta ccctgccccg cgtgcgcacg
     4021 catatccgtg attacgttcg caatgacgtt tatctcaaca acttgcaggc gaactacttt
     4081 gccgacttag gcaatggttt ctatggccag gtgtatggcg gttatctgga aacgatgtac
     4141 gccggtgtcg gttccgagct gctttatcgc ccgctagatg ccagctgggc gctgggtgtg
     4201 gacgttaact acgtgaagca acgtgactgg gacaacatga tgcgcttcac cgattattcc
     4261 acgccaactg gtttcgtgac ggcttactgg aacccgccga cgctcaatgg cgtactgatg
     4321 aaacttagcg ttgggcaata tctggcaaaa gataaagggg caacgatcga cgtcgccaaa
     4381 cgctttgaca gcggcgtggc ggtaggggta tgggcggcaa tcagtaacgt atctaaagat
     4441 gactacggcg aaggcggctt tagtaaaggt ttttatatct cgattccgtt cgacttgatg
     4501 accattggac ctaaccgcaa ccgcgcggtg gtttcgtgga caccattgac gcgtgatggt
     4561 ggacaaatgc tgtcacgcaa ataccagctc tatccaatga cggcagagcg agaagtaccg
     4621 gttggacaat aacctgacgt aattcataac attacattga ctgataaata taaaccaatg
     4681 aaaaagaaca tttttaagtt ttcggtactg acattggctg ttctgtcttt gacagcatgt
     4741 acccttgttc ctggtcagaa tttatcgacc agtaacaagg atgtcattga actacctgac
     4801 aatcagtatg acctggacaa aatggtcaat atctatcccg tcacgccagg attgattgat
     4861 cagttgcgcg ccaaaccgat tatgtcgcag gctaacccgg agctggagca gcaaatcgca
     4921 aactatgaat atcgtatcgg gattggtgat gtgttgatgg ttacggtctg ggatcacccg
     4981 gaactaacca cccccgcagg gcaataccgt agcgccagtg ataccggcaa ctgggttaac
     5041 gccgatggtg cgattttcta cccctatatt ggtcgcctga aagtggccgg gaaaacactt
     5101 acccaggtac gcaacgagat tacagcgcgt ctcgattcag tcattgaaag cccgcaagtc
     5161 gatgtcagcg tcgcggcgtt ccgatcgcaa aaagcgtacg tcaccggcga agtcagcaaa
     5221 tcgggtcagc agccgattac caatattcct ctgaccatca tggatgcgat taacgccgcg
     5281 ggcggtctga ccgcagatgc agactggcgc aacgtggtgc tcacccagaa tggcgtaaag
     5341 accaaagtga acctctatgc gctgatgcag cgtggcgact tgcgccagaa caaactgctg
     5401 cacccgggcg atattttatt tattccgcgc aacgatgatc tgaaagtctt cgtgatgggt
     5461 gaagtgggta aacagagcac gctgaaaatg gaccgcagcg gtatgaccct ggcggaagcg
     5521 ctgggtaacg ctgagggtat gaatcaggat gtcgctgatg cgacggggat ttttgttatt
     5581 cgtgcaacgc aaaacaagca gaatggcaaa atcgccaata tttatcagct gaatgcgaag
     5641 gacgcatcgg caatgatact cggaacggaa ttccagcttg aaccttacga cattgtttat
     5701 gtcactaccg cgccgctggc tcgttggaac cgtgtgattt ctctgctggt accgacgatt
     5761 tccggggtgc atgacctgac agagacttca cggtggattc aaacatggcc caactaaaat
     5821 ttaactcaat cctggtggtt tgtaccggca acatttgccg ttcgcccatt ggcgagcgct
     5881 tgctgcgtaa acgtctgccg ggcgtaaaag tcaaatcggc aggtgttcat ggtctgataa
     5941 aacaccctgc agatgcgaca gcggcagatg ttgccgctaa tcatggcgta tctcttgaag
     6001 gacatgccgg acgtaagctc actgctgaga tggcacgaaa ttacgatctg attctggcaa
     6061 tggagtcgga acatattgct caggttaccg caattgctcc cgaggtccgc gggaagacaa
     6121 tgctgtttgg gcaatggctg gaacagaaag agatcccgga tccctatcgt aaaagtcagg
     6181 acgcatttga acatgtctac ggtatgttgg agcgcgccag tcaggaatgg gcgaagcgcc
     6241 tcagccgata atttgagttt ataaaatacg atgacaacta aaaatatgaa tacgccacca
     6301 ggcagcactc aggaaaatga gatcgatctg cttcgtctgg tcggcgagtt atgggatcac
     6361 cgtaagttta ttatcagcgt gaccgcgtta ttcacgctga tcgctgtcgc ttactcgctg
     6421 ttaagcacac caatttatca ggcagatact ctggtccagg ttgagcaaaa acagggcaac
     6481 gccattctca gcggcctgag cgatatgatc cctaactcat cgcccgagtc tgcaccggag
     6541 atccaactgc tgcaatcgcg catgattctc ggtaaaacca ttgctgaact gaatctgcgc
     6601 gacatagttg agcagaagta ttttccgatt gtgggtcgcg gctgggcgag attaaccaaa
     6661 gaaaaaccag gtgagctggc gatcagctgg atgcatattc cacaactgaa tggtcaggat
     6721 cagcaactga cactcacggt tggggaaaac ggccactata cactggaagg tgaagagttc
     6781 accgtcaatg gtatggtcgg ccagcgtctg gaaaaagatg gcgttgcgct gactatcgcg
     6841 gacattaagg ccaaaccagg aacacagttt gtcctgagcc agcgtaccga actggaagcg
     6901 attaacgcat tgcaggaaac ctttaccgtt agcgaacgca gtaaagaaag cgggatgctg
     6961 gaacttacca tgactggtga tgatccccag ttgattactc gtattctgaa cagcatcgct
     7021 aacaactatt tgcaacagaa tatcgctcgc caggcggcgc aggattcaca aagccttgaa
     7081 ttcttacagc gccagttacc tgaagtgcgc agcgagctgg accaggcgga agaaaaactc
     7141 aacgtttatc gccagcagcg cgattcggtt gaccttaacc tggaagccaa agccgttctt
     7201 gagcagattg tgaacgttga taatcaactc aatgagctga ccttccgcga ggcagagatc
     7261 tcccagctgt ataagaaaga tcacccaact tatcgtgcgc tgctggaaaa acgccagacg
     7321 ctggagcaag aacgcaaacg cctgaataag cgggtatcgg caatgccttc cacccaacag
     7381 gaagtgttgc gtttaagtcg tgacgtagaa gcgggccgtg cggtatatct gcaattactt
     7441 aaccgccagc aggagttgag tatttcgaaa tccagtgcca ttggtaacgt gcggattatc
     7501 gacccggcag tcactcagcc gcagccagtg aaaccgaaaa aagcgttgaa tgtggtgctt
     7561 ggttttattc ttggcctgtt tatttctgtg ggtgccgtgc tggcgcgtgc gatgttgcgt
     7621 cgtggtgtag aagccccgga acaactggaa gagcacggca tcagcgttta tgccactatc
     7681 ccaatgtccg agtggctgga taaacgcacc cgtctgcgta agaaaaattt attttctaat
     7741 cagcagcgcc atcgtactaa aaatatcccc ttcctggcgg tggataaccc ggcggattct
     7801 gctgtggaag ccgtacgtgc gctacgaacc agtctgcatt tcgctatgat ggagacggag
     7861 aataacattc tgatgatcac cggtgcgacg ccagacagtg gtaaaacgtt tgtcagttca
     7921 actctggcag cggtgatcgc ccagtccgat caaaaagtgt tatttattga tgccgactta
     7981 cgccgtggtt attcgcataa cctgtttacc gtgagtaatg aacatggctt gtcggaatat
     8041 ctggcaggta aagatgagct caacaaagtg atccagcatt ttggcaaagg aggctttgat
     8101 gtgattactc gcggtcaggt gccacctaac ccgtctgaac tgctgatgcg cgatcggatg
     8161 cgtcaattac tggaatgggc gaacgaccat tacgatctgg tgattgtcga tacgccgccg
     8221 atgctggcgg tgagtgatgc cgcggtcgtg gggcgttctg ttggcaccag cctgctggtt
     8281 gcgcgttttg gcttgaacac cgccaaagag gtgagtttgt caatgcagcg tctggaacag
     8341 gcaggcgtca atattaaagg cgctatcctc aatggtgtga ttaaacgcgc cagcaccgct
     8401 tacagttacg gctataacta ttacggttat agttactccg agaaagagta aaactgaaag
     8461 tggccggatg cggcgtgaac gccttttccg gtctacgcat tagacagttc ttcgtttgtc
     8521 atcagcctca gagcattcag gtaactgaat gctctttttt atgcattaca aactgcacgc
     8581 cggtatgcgt gcttcattca cgatttgcgt aaaacctgcc aacgaacaca tgccctgcgc
     8641 atttcgctct tcacatcctg ccagggtcag tttcacctct ccgggcggcg tatttaatga
     8701 cagcggcgtt ttatcacgca tctgctgtaa agtctggaag accagcgaaa cctgaatcca
     8761 ctggctgtta tcgcttagcc gacgccagcg ttcaaacacc agttcaccac ctggcggcgt
     8821 gttatccggc tgaccgggaa gcgtccagtt gagctccagt gcgccgccga gatttgccag
     8881 attagtatcg tgtccggcga taaacagcac tgaagtgggt aatgtcacac catacgcctg
     8941 tttttgcggt ggatggggcg tcaacgctgt cttgatcaaa tctaataacg gggtggcgcg
     9001 gctgcgggca acctctggcg tgcgttgtag caaataaaat tgcgcgttat gcaaacttag
     9061 caaggtgttc cactggtgtg aatcggtgat ccttccccac cccggctccg gcattccctg
     9121 tgcttgttgc aggagaaata tctccgtcag cattgatgcg aggcttaccg caccggttaa
     9181 tgagacattg tcggcgctca ccttgagttc cgatggtaat gcctgcgtta atgaacagct
     9241 ttcgtcctgt ttctcacgtt taaggcacaa gtttgattgc ggaaaattaa gcacccgttc
     9301 cagttcgcga aacgccgttt gccgatgccc ggtaaagtca gcaattgacc ctcctgccct
     9361 gctgaggatc gcgtcagtca cgttcgcgtt atccagttgg caaacgccag tttttagagg
     9421 attaaataac ggatcgggac tggacgtatc agcctgggta tgtacggtta ttgcacagtc
     9481 aggtgccagc ccggcggcga aggcttcgcc tgttttacgg gtacgctcgt cgacatcagc
     9541 aataatcgcg acctgaccag actgcgggca gccctttttc gccagcaatc cgtcggctac
     9601 cagacgctgg cgttggtaat gtccgagata ggcgattagc tcaccaccgc gcggtgtcag
     9661 ccaacccagt tttaccggcc aggttggcca tgcgtctggg gtgacatcct gcatcagttg
     9721 cgtggccttg gttggagcac gcacaccatg acgactgaca atcaccacac tttccagctt
     9781 cagctccggc tcactctgag cgaatgcaga ttgcggggtt aacggaatca gaagagataa
     9841 aaatgggatt aagatcgctt tcatcgatat gtttccgctt ttcatatctg acattattga
     9901 ttgcctgatg cgatgctaat gcgccgcatc aggcctacgt tcgttaactt ttcagacgcg
     9961 ggtccagcca taccaacaca agggtggaga gcgaacacat caacaaaatg ccgacgaacc
    10021 aaagtaaata ccacattgtt cctgctcctt agtacaactc gttttcgtta cggcggagag
    10081 tttctgttgt catgcgtccc cacattttgt agtagctcca gagagtgtag agcaacacaa
    10141 tgggcaaaaa tatcagcacg attaccaaca taatgctcag cgtcagctga ctggaagtgc
    10201 tgtcccacaa cgtcaggctg gagattggac tcacgcttga cggcatgaca aaggggaaca
    10261 gcgtgatgcc tgccgtgaaa atcacgccaa attgcatcaa tgacgccatt aaaaatcccc
    10321 aacccgggcg accacgataa atcgccatca ccgtcagcaa tgggcagaag aaccccagca
    10381 gcgggaagat ccacaaaacg ggcgattcga cgaaattatt catccaggca ccaggtagca
    10441 ctgccaccag tttcattaac ggattggaag gaccgttagc atcctgggcg agcagtacaa
    10501 agccatcaat accgacccac agccagtaac ccgccagcaa aaagcacagc atcaccaaca
    10561 gtgcagcgcg tttggtggcc agctgtgaac gcagatgaat cacaccaaca gttttcagtt
    10621 gtaaccagac gccaccttgc aaaatcacca tcccaaggct gagcaatccg cacaataaag
    10681 ggaatggcgt cagcagttgc cagaagctgc cgagatactc cacgcgtaat tgcggtgtga
    10741 aggcaaacgg cacgccgagc aacaagttgc cgaaggcgat accgaagact accggcggca
    10801 ccagactgcc gatgaccaga ccggcgtccc acattttacg ccagcgtgca tcggcgattt
    10861 ttccgcgata atcaaaggcc agcgggcgga agaacagtga gcacagcacc aggatcatcg
    10921 ccacataaaa gccggaaaac gccgctgcat acactctggg ccaggcggca aataatgccc
    10981 caccagcgag gatcaaccag acctggttgc cttcccagtg tgcaccaacg ctgtttatca
    11041 ctatccggcg ttcatcatca ttacgcgcca ccagcggcag cagacagccg atccccatgt
    11101 caaatccgtc ggagatcata aagaccacca ggatcacgcc aatcagcagc caccagatga
    11161 agcgcaatgt ttcataatca aacatgattt tcccctttac ccctgttgct gcgtcggttg
    11221 ttcactctgc atcgcgctcg gccccagacg ggcatatttc tgcatcaggt agacttcggc
    11281 gattaagaac agggtgtaaa gccctacgat catgatcagt gagaaagcca gttgtcctgt
    11341 ggttaaagcg gagtgcgcgg agtatgtcgg taagatgtcc tgtatcgccc acggctgacg
    11401 accaaactcg gtcataaacc acccggcttc aatcgcaatc cacggcaacg gcaaactcca
    11461 gagcgccatt ttcagcaccc agcgatgctg gtcgattttg ccacgcagcg tctggacaag
    11521 cgcaatcagc atcaccagta gcggcaggga accacagccc accatgatgc ggaaactcca
    11581 gaataccggc gcaacctgag gtatcgcgcc acgcatcgcc gcctggtact gtgcggctgt
    11641 gacatgattc atatccggcg catagcggga gagcaacatg ccgtagccca ggtcaccttc
    11701 cagttcccgg aatgcctgca acacatgcgg ctcacgattg ccttgcgata tttcctgcat
    11761 taacagccag gccatacgtc cgcgttgcaa gcgtgggtag gtttcagcca tcaaattttt
    11821 cagacccggc acggttttat ctaatgagtg agtggcgagg atccctaaca gcgcgggaat
    11881 tttgatggca aaggcgttac gctcttgatc ctgttccggc caggcaacca catggaacgg
    11941 tgcaggagca ggttccgttt gccactcccc ttccatcgcc gccagtttta ccggttgtac
    12001 ttgcgcgact tcatacgcag aactgtctcc gagttgtagc gtgccgataa tcgccagagt
    12061 accgaagacg gaaccgatgg caaacgagcg taatgcaaca tcgcgctccc gtccgcgcag
    12121 taaataccag gcgctgatcg ccataataaa catggccccg gtcacgtagc ccgccattac
    12181 ggtgtgcaca aatatcacct ggctgaccgg gttaaagacc agctcgctga agctggtcat
    12241 ctccatacgt agggtgtcga tatcaaaatg cgcaccggtc gggtattgca tccaaccgtt
    12301 ggcattcaat atccacaatg cagagagatt tgaaccgaac gccaccagcc acgtcaccag
    12361 caggtgctgg tatttattca gccgctgcca gccgaagaag aaaagcccga caaaggtgga
    12421 ttcgaggaag aaggccatta atgcttccat cgccagcggt gcgccaaaaa tatcgcccac
    12481 atagttggaa tagaatgacc agttagtacc aaactgaaac tccatggtca ggccggtagc
    12541 cacgccaaga gcaaagttga taccgaagag cttaccccag aagcgcgtca tatcgcggta
    12601 gattgttttg ccggtgacca cgtaaatggt ttccatgata gccagcaaaa aaatcagccc
    12661 cagggtaagg ggtacaaata aaaagtgata cagcgcggtc agagcaaact gccagcgcga
    12721 taaatcaatg acatcccaca tgcgcactcc tgtaggcgaa gagagaaaaa taacccgata
    12781 atgttagatc tccctcttta tcctgaaact ctacgaccag aggtattcgc cgattgcgat
    12841 attcataaga ctgaaagcga tacttaccgt ctttttacgt cggtgcagct tccgccagcc
    12901 actgacatac ctcgctaagc cgctgcgcag agtcgaccaa atcttctggc gctgccagca
    12961 ccacttccgg tattgggcag atttcataac tttccagtaa aggacctttt aaggtgtccg
    13021 tacagcgtaa atgccagaca tggcgtaacc ccgtggcgtt gatatagctc tcgccatagc
    13081 caatggtacg aatctgaata tttcccggcc cacagagacg tgagagaaaa agccgatcag
    13141 cctcgctgat gggtaattgc gtcagattaa tgctgtgcgg ctgcgcgtcg gggttacgta
    13201 catgcgccag taactcatgc gccaacggta ggccattcat caggccatca atgggcggcg
    13261 gtaacagcga atctgtcggc aagagatttt gcgtggccgc ctgccacaac gccagcggcg
    13321 cgcagccggc ctccagtttg tcctccagca acttttcgcc gcgacgtttg cgcacccgcc
    13381 ataatccgca gaagatcgcc tcctgtattt cactttcgct gtcgtcagcc tgctgaatgc
    13441 gcactgacac ttccccttcc ccgagaagcg tattgataaa gtgacgatcg tcggcgttga
    13501 gatttaacag atcgactgcc agcacctcgc ccaacggcgg ctggcgttga cttagttgat
    13561 gttgtaactc gtttaacagc gcaatcacct gcgggctgtg agcacattgc tccagggccg
    13621 ccatactcgg ttcatcattc acctgacagc tgatcggcag tggattcatg ctgaaactgt
    13681 catcgttcgg ttgcgttcct ggccccagca gatggaaaaa agtttcgctc atgaggcacg
    13741 ctcctgttgt ggttcgacaa gcccacgcat caggtttatc agttccgctc acgggtgaat
    13801 accattcagc acaccgcgat agtttccgcc ggtaaacact aatgtggcgg gaaagcgaaa
    13861 gacgccaaag cgatctccga tggcttcgct ctgttcaagg tcagcaatcg ccacctgcca
    13921 tgtgtagccg ggaaactcgc gcagtaattc gccaatcatt accggattat cgctgacctc
    13981 tggcgtgcgt ttcgggtcac tgcttaataa caccacgccg tctggcgctt gcgtaagcca
    14041 gtcgtcaaga cggaattcac taaccggcgt ccagccgcgc gccagcattc gttgccacaa
    14101 cgcatcaaat ggcgtgtcgt tgctcatccc tgctcctcca gtgtcatcct gtactggcgc
    14161 aagcgaacgc cttcgtaatt ttccatcgac agacagtcat aattgagaca gcgcgattca
    14221 ttagccggtt gcggcacaat tccccatgca gccagctgcg ccagcgccgc ctgttccgct
    14281 gcgggcagtt gctcccgtgc cagttcgctc aggctgccgc cgtagtcgtc gagcattgcg
    14341 ggttgcagac cgacgagggc aatatgtgct ggcagatgtc cgcggatatc tgccagcgcc
    14401 agcacttcgg agaaactgtt ctggtgcagg ctcattttct tcgcgctgag ataagccgga
    14461 atacgttctc cggcataggt tcgcagcgtt ccaggttcca gcccgtagtc aatggcatcg
    14521 agaatcaaca gatggctggc gctttcgaca taccccagca agttcagtcc ctgagtaccg
    14581 ccatcgacaa tctccacata ctcgggccag tggtaatggg catacaaccg ttccgccacc
    14641 cgcacgccga aaccttcatc ggcccacagc aggttgccca gccccattac cacaacgcgt
    14701 tgctcgctca tgaacgctcc ttgttactta ttttgccaaa tttgtggcta cggtagccgt
    14761 tgaccatggt ggagatcacc gtgtcgtcgg acatgatgtc ttcacgcagc gccatataga
    14821 catgaccgat cacaaacgcg ccaatcagcc acatccccag ccgatgccag ctgtgaatgt
    14881 ccattgagtt gcctcccgtc cagtagaaaa attccaccac ataacggaac ggcgcaaaaa
    14941 tagcgtactg gctgtgttcg ctgtacagcg caaagccggt gatgatcata aagaccgaca
    15001 tcaggaaata gccgaacatc gccgcctggg cgatggggtt atggcctata tcggcactcg
    15061 gacgttttgc cagaacagat accagcggat ttcataccac acgccctgcc accagctttt
    15121 acgccatacc ggcacgataa acagctcgcg ggagtatcga ttgccaacaa aagcccagta
    15181 gatccgcatc agcaagacca cggtaaaaac catcccggcg ctgaagtgaa ttaacctgat
    15241 gtagcccata tagaacagat acgtcgcctc gccgctgacg gaaggtagcg gcttgccgat
    15301 aaagtatccg gtgaccatca acaccgccat gcataacacc gtcagccagt gccagatgcg
    15361 gactggcgct tcaaagacat agtggctgac aacgttgtcg cttttctgtt gcatgatgat
    15421 tctccttcgc tgttaacgca cctgcacgga gatcagctcg ctaccgtcgt cgcccagcac
    15481 gtgtgttgaa caggcgaggc acgggtcaaa gctgtgcaga gtacgcagga tctccagcgg
    15541 ttgctcgggg atcgccattt tggtgttcat cagcgccgct tcataagcgc caatctgccc
    15601 tttgggatcg cgcgggctgg tgttccaggt ggtcggcacc acgcactggt agagatcaat
    15661 cttgccatcg cgaatggcgg cccagtggcc taacgccccg cgcggcgctt cggtaaagcc
    15721 gacgccacgg cactctgtcg gccaggttgc aggttcccat ttttccgtgg aggcggtggc
    15781 aagattgccg tttttcaggt tagtcatcag cttgttgaag aaatattgca acttacctgc
    15841 ggcccactgt gcttcgtgcg cgcggcacaa aatgcggccc aacgttgact ggataccgga
    15901 aagcggcagg ttcagcgccg acatcatgcg atcgaccgac tcaacggtcg cagcatcgcc
    15961 tttgtgataa gcgattaacg tgcgtgccag cggccccact tccatcgcgt taccgcgcca
    16021 gcgcggcgct ttgatccacg agtagcgttc ctgttcattc agctgctgaa tgttggtatc
    16081 gctgcctttg acatcgccgg ggttgtacca cgggtcggta atgccatcga atggatgacg
    16141 cccgacctga tcgttgggat aacgatacca ggcgtggtcg acaaactcct gcacctgctg
    16201 cggatcaacc aaatccactg gcagcacatt gttgaagtcg ccgttaatca ccgcgccgcc
    16261 aggcatcagc agacttttct caccaaagtc gttggcaata tccgggaacg caccgtagct
    16321 gaggacacat ttatcagaaa gacccgtgcc gatttcgctc cacggtttgt tgaactgacc
    16381 gatggctaag gcgtcgggga tcatcacgtt gttaatgaag tccgccgtgc gggtgataat
    16441 cgactgcacc aggttcaggc gttccatatt gaccgccccg accgcgccgc tttcgtcaat
    16501 gttaatggcg caaggcatcc cgccgacaat ccagtttgga tgcgggtttt taccgccaaa
    16561 gaccgcgtgg attttgacaa tttcacgctg gaaatcgaga gcttcgagat agtgggcaaa
    16621 gcccatcagg ttagcttctg gcggcagttt gtactgcggg tgcccccagt agccattgcg
    16681 gaagatcccc aactgcccgc cttcaacaaa ttttttcagg cggttttgta cgtcgaagaa
    16741 atagccaggg gatgatttcg gccaagagga gagactttgc gccagttcgg aggttttccg
    16801 cgggtcggct ttcagcgcat ctaacacatc gatccagtcc atcccggcaa gctgatagaa
    16861 gtgcaccaga tgatcgtggc accagagcgt tgccagcata atgttgcgga tgatattggc
    16921 gttgtccggc actttaatac cgatagcatc ttcgatggcg taaaccgaag ccagggcgtg
    16981 tacgccagta cagacgccgc agatacgttc aacgaacgcc cacgcatcgc gcgggtcgcg
    17041 cccttgtagg atgatctcca gcccgcgaaa catggtgccg caggagacgg cattggtgat
    17101 cacattctga tcgttaatat tcacttcgca gcgcatgtgg ccttcgatgc gcgtaatcgg
    17161 gtcgaccacc aggcggcgtc cggcattatt gatggtgtat ccctgagttt cgtactgagt
    17221 gctcatgcct gtttatcctc attgcctggc tgatgttcgg tttctgtagg ttgctggtta
    17281 tgacgtctgc gctggtcaac ggcgctggcg actgcgtgca caccaacagc cgctgccacc
    17341 acaccaagcg cggttaaacc gacggtatcg gcggtggaat gagtacccat ttgcggaata
    17401 tcgaccacgc ggctgtagaa cgaaccgcaa tcccagaaac cattttccgc acagcccagg
    17461 cagccgtgac cagactggat tgggaaagaa acgccatcat tccagcgtgt ggaggaacag
    17521 gcgttatagg tggtaggccc tttgcagccc attttgtaca ggcagtaacc tttgcgggca
    17581 gcgtcatcat cccaactctg gacgaactct ccggcgtcga agtgggcgcg gcgatagcat
    17641 ttatcgtgga ttcgctgacc atagaacatc agcggacggc ccattctgtc gacatctggc
    17701 aagcgatcaa aggtcaccat gtaagtaatg atggcgctca tcacatccgg gatcggcggg
    17761 cagccaggta ctttgataat gggtttgtcg gtgatgactt tgtcgatagg cgttgcctgc
    17821 gtcggattgg gccacgcggc ctgcacgcag ccccaggacg cgcaggttcc ccaggcgata
    17881 atcgcgctgg ctccggcagc ggcacgtttg agtttctcaa taaacggtcg accgctgctg
    17941 atacagaaca tcccctgctc gcccagcggc ggattacctt ctactgcgag gatatatttg
    18001 ccattgtatt gcgtgatgat gtcttcgaag acttcttccg cctgggttcc ggcggcagcc
    18061 atcaaagtat cgtcataatc gagggaaatc agggaaagga tgacgtcctt cgccagtggg
    18121 tgagcggagc ggataaaaga ttcggtacag caggtgcatt ccagaccgtg gatccatacc
    18181 accggaatgc ggggtttgtt ctccagcgcc caggcaatct ttggtgccat tcccgcgcct
    18241 aatcccagcg acgtggcagc cagactacaa tatttgagaa agctgcgccg ggtaacgccc
    18301 tgacgccgca tggcctggta aaatgtttcc tcgttattca tatcgcacgt ctcttctcct
    18361 tgcgacaccg gcaggacacc gggcgcttat ggtcggcaag cgacgtccct gtagcaaggc
    18421 aaaccatcgc ttcacgtgag atgctgaaaa cgaaagctca tccttctgca ctggcgcacg
    18481 tcgccagaaa gtattgttaa taaagcgtag tgaaa
//
LOCUS       DAEEKQ010000074        18214 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.074, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000074 DAEEKQ010000000
VERSION     DAEEKQ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18214
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.074"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            15..1091
                     /locus_tag="KLK57_002405"
     CDS             15..1091
                     /locus_tag="KLK57_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
                     /protein_id="HBE0892755.1"
                     /translation="MQRKKYDPNLPRNLTYRRRDKAYYWRNPLTKEEFTLGKISRRDA
                     VTQAIEANHYIYKNYSPAALIEKLKGFDSFTMADWIERYKTILIRRKVSRNTYKIRAN
                     QLETIKEKLGEILLTEITTRHIAEFLDLWIEGGKNTMAGSMRSVLSDMFREAIVEGRI
                     SQNPVTPTRAPKIVVTRERLKLKIYNCIREAADQLPAWFPLAMDLALVTGQRREDITN
                     MRFSDIYDDRLHVRQIKTGMMIAIPLSLSLPVAGLRLGTVVERCRLVSRGDYLISAGI
                     RKNSPDGSIHPDGLTKKFVAARKFTGIQFSENPPTFHEIRSLAGRLYKETCGEEFAQR
                     LLGHTSEKTTKMYLDEREKTYLLL"
     gene            complement(1484..1825)
                     /locus_tag="KLK57_002406"
     CDS             complement(1484..1825)
                     /locus_tag="KLK57_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HBE0892756.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(1838..2710)
                     /gene="copD"
                     /locus_tag="KLK57_002407"
     CDS             complement(1838..2710)
                     /gene="copD"
                     /locus_tag="KLK57_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HBE0892757.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWTDVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNMPRLLFMLTTAQFILLAGMGHATLNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMCFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQEEDRAAPWFVWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="KLK57_002408"
     CDS             complement(2714..3088)
                     /gene="yobA"
                     /locus_tag="KLK57_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HBE0892758.1"
                     /translation="MASTARSLRYALAILTTSLVTPFVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            3227..3457
                     /gene="holE"
                     /locus_tag="KLK57_002409"
     CDS             3227..3457
                     /gene="holE"
                     /locus_tag="KLK57_002409"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HBE0892759.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            3559..4215
                     /locus_tag="KLK57_002410"
     CDS             3559..4215
                     /locus_tag="KLK57_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HBE0892760.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            4239..4901
                     /gene="exoX"
                     /locus_tag="KLK57_002411"
     CDS             4239..4901
                     /gene="exoX"
                     /locus_tag="KLK57_002411"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HBE0892761.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="KLK57_002412"
     CDS             complement(4898..6958)
                     /gene="ptrB"
                     /locus_tag="KLK57_002412"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HBE0892762.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPCENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTDERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDNHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(7167..7826)
                     /locus_tag="KLK57_002413"
     CDS             complement(7167..7826)
                     /locus_tag="KLK57_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HBE0892763.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(8153..8509)
                     /locus_tag="KLK57_002414"
     CDS             complement(8153..8509)
                     /locus_tag="KLK57_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HBE0892764.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="KLK57_002415"
     CDS             complement(8576..8866)
                     /gene="yebG"
                     /locus_tag="KLK57_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HBE0892765.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            9000..10178
                     /gene="purT"
                     /locus_tag="KLK57_002416"
     CDS             9000..10178
                     /gene="purT"
                     /locus_tag="KLK57_002416"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HBE0892766.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVADIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWKYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="KLK57_002417"
                     /gene_synonym="eda"
     CDS             complement(10234..10875)
                     /gene="kdgA"
                     /locus_tag="KLK57_002417"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HBE0892767.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(10912..12723)
                     /gene="edd"
                     /locus_tag="KLK57_002418"
     CDS             complement(10912..12723)
                     /gene="edd"
                     /locus_tag="KLK57_002418"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HBE0892768.1"
                     /translation="MNPQLLCVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSLGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEQ
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="KLK57_002419"
     CDS             complement(12958..14433)
                     /gene="zwf"
                     /locus_tag="KLK57_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HBE0892769.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            14771..15640
                     /gene="hexR"
                     /locus_tag="KLK57_002420"
                     /gene_synonym="yebK"
     CDS             14771..15640
                     /gene="hexR"
                     /locus_tag="KLK57_002420"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HBE0892770.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRTVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            15768..17210
                     /gene="pyk"
                     /locus_tag="KLK57_002421"
     CDS             15768..17210
                     /gene="pyk"
                     /locus_tag="KLK57_002421"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HBE0892771.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(17270..18112)
                     /locus_tag="KLK57_002422"
     CDS             complement(17270..18112)
                     /locus_tag="KLK57_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HBE0892772.1"
                     /translation="MHHIKTAADLNCFLNSMVAVSQPTVDKIIFGAEATLINKIGTCW
                     IASMDVLRKAVFEGVNIIITHEPTFYSYADLEGDDLEFSWARKIMDYTRGELSYLKII
                     EQKKEFLHKNNLVIIRCHDVMDREPTFGMSKALAQQLELDVTNIVASDEMYCVYAIDP
                     DSAINITKRFAKNLKIYNLPGIQFYGDKARVVRTVGIGAGCFCDPIQYMEYQADYYIT
                     INDSIKTWVQTQYSKDSGLPMAVIGHSVAEEAGMRRLASYLDLHSGYPCIHFTGGCDY
                     DWIE"
BASE COUNT         4672 a         4575 c         4391 g         4576 t
ORIGIN      
        1 ggagaagtta aaacatgcag cggaaaaaat acgatcccaa tttgcctaga aacttaacat
       61 atcgaaggag agacaaagca tattactggc gcaaccctct gacgaaagaa gaatttacac
      121 taggtaaaat ttcaagaaga gatgcagtaa cgcaggcaat tgaagcaaat cattatatat
      181 acaaaaacta ctctcccgct gccttaattg aaaaacttaa agggttcgac tcatttacta
      241 tggcagactg gattgaacgt tacaaaacga ttcttataag gagaaaagtg tccagaaata
      301 cttataaaat tcgggcaaat caactggaga caataaaaga aaaattggga gagattttac
      361 tgacagaaat aaccactcgc catattgccg agtttcttga tttgtggatt gaaggaggga
      421 aaaacacaat ggcaggatca atgcgttctg tgttgtctga tatgtttcgc gaggccattg
      481 ttgaaggacg tatatctcaa aatccagtca cgccaacaag agcaccgaaa atagtagtta
      541 caagagaacg actgaaacta aagatataca actgcatcag ggaggcagca gatcaacttc
      601 cggcatggtt cccattagct atggatttag cccttgtaac aggacaacgt cgcgaagaca
      661 taacgaatat gcggttcagt gatatttatg atgatcgtct ccacgtcagg caaattaaga
      721 caggaatgat gattgctatc cccctgtcac tcagccttcc tgtcgctggt ctacggcttg
      781 gtacagtagt tgaacgatgc cgcctggtaa gccggggaga ttatctaatc agtgccggga
      841 ttagaaaaaa cagccctgac ggcagcattc acccggatgg cctgacaaaa aaatttgtcg
      901 cagccagaaa attcacaggt atccagttca gtgaaaaccc accaactttt cacgagatca
      961 gaagcctggc tggacgattg tacaaagaaa catgtggaga agaatttgct cagcgtctac
     1021 ttggccacac atcggagaag acaacaaaaa tgtatcttga tgagagagaa aaaacgtact
     1081 tactgctctg attttaacgt aaatggattg ttaaatgtat tttggttgtg atataaccaa
     1141 aaagaccgga atacagaaat tcgagtaaat ttcggggaat ttcgggggag acatttgcaa
     1201 ctaattgatt tcaaatgcaa ttaaaaaaag accgaatacg attcctgtat tcggtccagg
     1261 gaaatggctc ttgggagaga gccgtgcgct aaaagttggc attaatgcag gcttagttgc
     1321 cttgcccttt aagaatagat gacgacgcca ggttttccag tttgcgtgca aaatggtcaa
     1381 taaaaagcgc ggtggtcatc agcttaaatg ttaaaaaccg cccgttctgg tgaaagaact
     1441 gaggcggttt ttttattgga aatcaaaagg ctattttagg taattaacag agtttttcag
     1501 ctcgttctat aaacggtgcc agactcattt tttcgccggg attgttagga tcatcaatct
     1561 gaatcaccga aatgggttgg gcattggtct tcccactggc aacttccttt tgtgcgatat
     1621 cgtttaaagg atactgcacg agggtactcg gattaataac atacaaagca ttacccggtc
     1681 ggcaagtcag catcacctct tcgcgattaa acgcccattt gtctttaccc acttcaaaac
     1741 gactgacggt aatcacctgc ggtgcagcca gcgccgctgc agaactggtg agtaacagaa
     1801 acgccagaat actttttttc atcattttcg taatccatca aaaaggttcg agagtcgcaa
     1861 gcaggctgat gatcaccagc actaccgcac ctatcgccca ttcgagttta gtcatccaca
     1921 caaaccaggg tgccgcacga tcttcctcct gcctcatgcg cggtacaaga acataacgat
     1981 tagccagcgc aatgaccacc ataatcatca ccaggatggc tttgagcaat aagagttggc
     2041 cccagtaagt cgtaagcgtg gggggaaatc cggtaatcaa caatgcatta agcacgccgc
     2101 ttgccagtac gccgatcacc gcaaaatgcc cgcaccatga aaaacacatc agcgcctgaa
     2161 tagcctggtg tcgccaacgg cctttgatga gttgcataca ccagagcacc ggcagtaatc
     2221 ccccaaacca cgccgccgca caaatcaggt gaatcgcgtg attagtctga tggattttcg
     2281 ccgttacccc ttcattcagt gtcgcatgcc ccattcccgc cagcagaata aattgcgcgg
     2341 tagtgagcat aaacagcaaa cgtggcatat tccgcggttg cataagggcg acgatcaacg
     2401 ttacgagggc gagaacaatt tgccataacc agacgccacc aaactgcgtt tgcaaaacag
     2461 cctgccaaat gtttggcgaa aatacatctg tccatcccgt tcccatcaac ccgccctgaa
     2521 cggcgagcat tgcagtggca ctgatcaaac tccagacggc agcgtgctgt tgtaaacgca
     2581 gaaaacgctt cgccagcaag cgacgaatcg tcaagggggc cagccaggca ccgtacattg
     2641 cgaagccaaa aaccagcatc agcgaggtga aatggataaa tcgcagcgcg atccaggtaa
     2701 acgccagcat gatttatttc acgctaaagg tgtagtgtcc tttcgtttta tgcccgtcca
     2761 ccgaaacaac atgccagtca acggtgtagg ttccgggttt cagcgaatcg gccagtggaa
     2821 caatcagttg cttttggtcc tgctcatttc gcttcgccgg taatgtttta atattttcgt
     2881 tttttggccc cgtgattttt gcaccactga atcctgtttc cacaccttcc gagaagttta
     2941 aagtgattgc ctgcggtgca gctgtcactt gcgcgtttgc cgcaggatac tgatgcgtta
     3001 aatgcgcatg tgcccagacg aaaggtgtca ccaacgaagt ggtcaggatt gccagcgcgt
     3061 agcgaaggga gcgtgcagtt gaagccatat tatctattcc tttttgtaat aactttttta
     3121 cagagcataa ccttgtctaa tgtctgagtc gaggatcatc aattccggct tgccatcctg
     3181 gctcactctt agtaactttt gcccgcgaat gatgaggaga ttaagaatgc tgaagaatct
     3241 ggctaaactg gatcaaacag aaatggataa agtgaatgtc gatttggcgg cggccggggt
     3301 ggcatttaaa gaacgctaca atatgccggt gatcgctgaa gcggttgaac gtgaacagcc
     3361 tgaacatttg cgcagctggt ttcgcgagcg gcttattgcc caccgtttgg cttcggtcaa
     3421 tctgtcacgt ttaccttacg agcccaaact taaataaaac ttatacaggg ttacactttc
     3481 ttacataacg cctgctaaat tatgagtatt ttctaaaccg cacccataat ttgcagtcat
     3541 tttgaaaagg aagtcattat gtcgttctgg aaagttgcag cagcgcaata tgagcctcgt
     3601 aaaacctcac tcaccgaaca agtagctcat catctggagt ttgtgagggc cgccgccagg
     3661 caacaatgtc agctgttagt ttttccatcg ctttctttac tggggtgtga ttattcgcga
     3721 cgtgcccttc ctgccccacc cgatctctca ctgttagacc cgctttgtta tgccgcaacg
     3781 acctggcgaa tgaccatcat tgccggcctt cccgttgaat ataacgatcg ctttattcgt
     3841 ggaattgcgg tgttcgcacc gtggcgaaaa acgcctggga tctaccatca gagtcatggt
     3901 gcatgtctgg gccgtcgttc cagaaccatc acggtggtcg atgaacaacc ggaaggtatg
     3961 gatatggacc cgacctgttc actgtttacg acagggcaat gtctgggaga acccgacctg
     4021 ctggcgtcgg cccgtcgctt acaatttttt tcacatcagt actcgattgc tgtgctgatg
     4081 gcaaatgccc gtggtaacag tgcactatgg gatgaatatg gtcgtctcat cgttcgcgcc
     4141 gatcgtggtt cattattgtt agtcggtcag cgttcatcac agggttggca aggcgatatc
     4201 attccattac gctaggcttt ttcggcctgg agtatgccat gttgcgcatt atcgatacag
     4261 aaacctgcgg tttgcaggga gggatcgttg agattgcctc tgttgatgtc attgacggaa
     4321 aaatcgtcaa ccccatgagc cacctggtgc gccccgatcg tcctattagt ccacaagcga
     4381 tggcgattca tcgcatcacc gaagccatgg tcgccgataa accgtggatt gaagatgtga
     4441 tcccacacta ttacggcagt gaatggtatg tcgcgcacaa cgccagcttt gaccgccgcg
     4501 tactgcctga gatgcccggt gagtggattt gcaccatgaa actggcccgt cgcttgtggc
     4561 caggaatcaa gtacagcaat atggcgttat ataaaacacg gaagctcaat gtacagacgc
     4621 cgccgggcct gcatcatcac cgcgcgttgt atgactgtta tatcaccgcc gcgttgctta
     4681 tcgatattat gaacacctcc ggctggacgg cagaacagat ggccgatatc accggacgtc
     4741 cgtcgttgat gacgaccttc acctttggca aataccgtgg caaagcggtt tccgacgttg
     4801 ccgaacgcga tccgggctat ctgcgctggt tatttaataa cctggacagc atgagcccgg
     4861 agctgcgttt aacactgaaa cattatctgg aaaatactta atccgcaggc gtagcgggta
     4921 atgttccctg cgccagcgcg accagaaaag catattccat cgctacgcct tcgtacgatt
     4981 taaagcggcc agatttaccg ccatggcctg agtccatgtc ggtacagagc aataaaagat
     5041 ggttatcggt tttcagctcg cgcaatttag cgacccattt tgccggttcc caatattgca
     5101 cctgagaatc gtgtaaaccg gtcgttacca gtaaatgcgg ataagcctgt gcggtgacgt
     5161 tgtcatacgg gctgtagctt ttcatgtact cgtaatattg cggatcctgc gggttacccc
     5221 actcttcaaa ctcaccagtg gtaagaggaa ttgattcatc aagcatcgtt gttacaacat
     5281 caacaaacgg tacctgggcg ataacgccgt ggaataattc cgggcgttga ttaattgcaa
     5341 cgcccatcaa catgcccccc gcactcccgc ccatcgcata acaaagcgaa ggagagccat
     5401 agcccagttt taacaatgca tcgcaggcat caagataatc attaaacgta tttttcttct
     5461 tcagaaattt tccgtcttcg taccattgtt gccccagctc accaccgccg cgaacatgga
     5521 caatggcgta gacaaagcca cgatctaaca aactcaagcg gctaaaactg aaatcggcat
     5581 caatacttgc gccgtaagaa ccatagccat acaccagcaa cgggttgtgt cctttgcgaa
     5641 aatgtttgcg atggtagacc aacgaaaccg gaacttcgac gccatcacgg gcgactatcc
     5701 acaggtgttc actgcggtaa ttcgccgcat caaaaccagg aacttccgtt tgttttaata
     5761 cacgacgctc atcggtatcc atatccagtt caaacaaagt gtctggtgtg gtcatggaag
     5821 aataaccata acgcaatcgc gcggtttcag gttctggatt gtaggcaatc caggtcacat
     5881 aggctggatc atcaaaggca ataccaatga cttcccgggt cttgcggtta atttggcgca
     5941 aactggttaa cccgcgctga cgctcttcaa ccaccagcca gtcggtaaac agcgtaaacc
     6001 cttccagcat gatgttttcg catggcggaa ttaactcttc ccactgttgc tcatcacgca
     6061 tacgggtacg gtataagcca aagtttttgc cgtggcggtt ggaacgcaga taaaaacgat
     6121 gctggtagtg atcaaggctg tattcgtgat ctttgcggcg cggcagaaaa acaaacggct
     6181 cggcatcggc catttccgcg tccagcaggc gaacttcact ggtggtggcg ctggccaaat
     6241 gaatgactac atagtgcttc gaggtcgttt tatgcaggct gacgtaatag gtatcgtctt
     6301 tttcttcgta gatcagttta tcttgcgatg ctggcgtacc gatggcgtga cgccagacct
     6361 gataaggcag cagcgtcacc ggatgcttgc gaacatagta gaaagtccag gagtcatttg
     6421 cccagacaaa gctgggttca acgttatcca gcagttccgg gtaccagtta ccagtttcca
     6481 gattacgaaa acgaatgccg tactggcgtc gggaaagaaa atcttctgcc agcgccataa
     6541 tggtgttatc gggcgtaatc gccattccgc ccatcgaata aaactcacta tgagctgcgc
     6601 gcttattggc atcgagcaat atttcccact catcccactc ttcactgaat gccgattgac
     6661 gctggtagat agcatattca cagcctggtt cataaatatg ccgatagcgg tagccatttt
     6721 tgatgtaggg cgcagaaact tctcgttgcg gaatgcggtc gatgatttcc tttaagatgc
     6781 gatcctgcaa ggcttgttgt gaggccatca cccgatgacc gtaactattt tcttgttgca
     6841 ggtagtccag gacttctggc tgagaacgcg tatcatcccg cagccagtag taattatcga
     6901 tgcgcgtatc gccatgaagc gtcatggcgt ggggaatgcg ggcggctttt ggtagcatgt
     6961 tattgttctt tctggttgaa acatcttata agggtggcaa aactcaccgg ggatgcaagc
     7021 gaaacagggg agtcattgct tagatgatga caggtaatgg cacggatatc gaatgttatg
     7081 cagacagaga aactcagcct gttcaaatgg ctgtgcggtt ctggatagcc cgaaatagtc
     7141 aacttcaggc tatccagaga gcggaattat tccgccaaag tgcgtttttg ctgttcgaga
     7201 tcgcgttcaa tgccgtcacg aacatcctgg gggattttca gcgcgtcacc cagtgcattc
     7261 aggtaactgc gttccataaa atggtcaata tcaatagccg cgcaactcag gaaatagatt
     7321 tccagcgcct cttcttcatt gcggaccccg gtagccaggc gttgtggatc cagcggttgt
     7381 tcgattgcct gctcaatgag tacacgcccc tgctcttcca cgccggcttc acgcaattgc
     7441 tggtcgattg ccgcacgttc tttggcatca atatgaccat cacttttagc ggcaaagacc
     7501 agcgcaagga tcaaacgttc tgtacgctca tccagcggcg tactttgcgc gccaaactgc
     7561 ggttcgtcct gatgcgccgc gcgaatttta tctttgtatt tattccacag caccgtaccc
     7621 gctaccgctc cgccgccaac cagtaacgcg ttggtgccat atttcgtaag aagtttacgt
     7681 gctgatttat ttgcgaccag cagcccagcc agtccgccta atgcgcctgg cactaacagt
     7741 ttgcccaatc cctgatccgc agacgaggag gtagaagaac tgctttgccc aagcagggat
     7801 tgcagttgat ttaaccagtt agccatattt gctcctcaat aaccattttt agtgcctgta
     7861 gcatagcgga tgaggatgtc agaaaatgta tatcgagaca aaagatgcgc aaataagcgg
     7921 ggcggtaggc cgttattcaa agaaattcgc gccaggtaag gtatttactg tatatcaggt
     7981 gataagggta ttttaggtga aagtagtttg tcgttttgag tggcattgtt tgatgctgat
     8041 tgcccgatgc gacgctgttg cgtcttctca ggcctacgat tgtggtgcgt tctggggcgg
     8101 acaaggcact cgcgccgcat ccaccaacgg tcaatcgaat tatcagaagc cattaacgcc
     8161 gctgatattc cgccattccc gctttgcagt ccacgctgac ctggtaatga atatcggcac
     8221 ttttaccacg cacggttagc ggtactgacc atttatcatc tttaccctga atgtcctgca
     8281 aactgaccca agccacggga tcggcctgac cgacaatttt ttgatcatct gcccaacgcg
     8341 ccacgcgatt ttgttgataa tcacgtttta cgctcgcggc aattccggca gcatccagac
     8401 cttcacactt tgggaaagtg accgacttgc tggtttcatt attggcagcg aaaactgatg
     8461 cgcaggcaga aaccaacaac agccctaaaa acgcccctct ttttttcatg tttttctcca
     8521 tagcacaatg attcaggaga aagcatggta caaattgtca ggagcgcaag ttgcttcagg
     8581 ccgcgtgtga ggcatcttcc tcttcggatt cagcaccgac gacctgtggg gacggtaatt
     8641 tgccggtttt cagaatggtg ctcagcacat ctttttgttc tgccagccat agcgaaaggg
     8701 cttcacgttg ctcgtcttcc atttgcactg gagaatttgt cagccaggtg tcaagcagat
     8761 ccgccgtatc aagcattttg tcataagcat cggcttcctt tttgctggta aacgacattt
     8821 tctcttcgcc ctcacgaatg actacgtatt taacttcaac cgccatttgc agcctctcat
     8881 aataactgtg attttataca gtatatttct tttcggttga gaaatcaaca tcagcaataa
     8941 agacacacgc aaacgttttc gtttatactg cgcgcggaat taatcagggg atattcgtta
     9001 tgacgttatt aggcactgcg ctgcgtccgg cagcaactcg cgtgatgtta ttaggctccg
     9061 gtgaactggg taaagaagtg gcaatcgagt gtcagcgtct cggcgtagag gtgattgccg
     9121 tcgatcgcta tgccgacgca ccagccatgc atgtcgcgca tcgctcccat gtcattaata
     9181 tgcttgatgg tgatgcatta cgccgtgtgg ttgaactgga aaaaccacat tatatcgtgc
     9241 cggagatcga agctattgcc accgatatgc tgatccaact tgaagaggaa ggactgaatg
     9301 ttgtcccctg cgctcgcgca acgaaattaa cgatgaatcg cgagggtatc cgtcgcctgg
     9361 cggcagaaga gctgcagctg cccacttcca cttatcgttt tgccgatagc gaaagccttt
     9421 tccgcgaggc ggttgctgac attggctatc cctgcattgt aaaaccggtg atgagctctt
     9481 ccggcaaggg gcagacgttt attcgttctg cagagcaact tgctcaggca tggaagtacg
     9541 ctcagcaagg cggtcgcgcc ggagcaggcc gcgtaattgt tgaaggcgtc gttaagtttg
     9601 acttcgaaat taccttgcta accgtcagcg cggtggatgg cgtccatttc tgtgcaccgg
     9661 taggtcatcg ccaggaagat ggcgactacc gtgaatcctg gcaaccacag caaatgagcc
     9721 cgcttgccct tgaacgtgcg caggagattg cccgtaaagt ggtgctggca ctgggcggtt
     9781 atggtctgtt tggtgtcgag ctgtttgtct gtggtgatga ggtgattttc agtgaggtct
     9841 cccctcgtcc acatgatacc gggatggtga cgttaatttc tcaagatctt tcagagtttg
     9901 ccctgcatgt acgtgccttc ctcggacttc cggttggcgg gatccgtcag tatggtcctg
     9961 cagcttctgc cgttattctg ccacaactga ccagtcagaa tgtcacgttt gataatgtgc
    10021 agaatgccgt aggcgcagat ttgcagattc gtttatttgg taagccggaa attgatggca
    10081 gccgtcgtct gggtgttgcg ctggcgaccg ctgagagtgt tgttgacgcc attgaacgcg
    10141 cgaagcacgc cgccggacag gtaaaagtac agggttaaac ccggcaaaaa aacgctacaa
    10201 aaatgcccga tcgtagatcg ggcgttttga cttttacagc ttagcgcctt ctacagcttc
    10261 acgcgccagc ttagtaatgc ggtcgtaatc gcccgcttcc agcgcatctg ccggaaccag
    10321 ccaggaacca ccgatgcaca gcacgctttt cagcgccagg tagtcacggt agttagccgg
    10381 agaaatacca cccgtcgggc agaaacggac ctgggagaac ggacccgcga tcgcctgcag
    10441 ggctttcacg ccgccgttag cttcagccgg gaagaatttg aactctttca aaccgtagtc
    10501 catacccagc atcagttcgg aaacagtgct gatccccgga attagaggaa tagtcccttc
    10561 ggtagcagct ttcagcagcg gctcggtcag acccgggcta attgcgaact gtgcacccgc
    10621 ttcagtgact tctgccagct gctgtggatt cagcaccgta ccggcaccca caatcgcttc
    10681 aggcacttct ttggcgatag cacggatagc gtcaactgca cactcggtac gcagagtcac
    10741 ttccagaacg cgcaccccac cagcaaccaa cgcttttgcc atcggcactg cgtgttccag
    10801 tttttttacc acgataaccg gtacaaccgg gccggtggtc aggattgatt ctgcacttgt
    10861 tttccagttt ttcatcagag ttttctctcg cctgattaca aatttgtcgt cttaaaaagt
    10921 gatacaggtt gcgccctgtt cggcaccgga cagtttttca cgcaaggcgc tgaataattc
    10981 acgtcctgtc cccacgcgtg acgcgctcag gtcaggaatg tgcggttcgc gagcagccag
    11041 ttccgcttcg tctaccagca gcgtcagttc gcctgtctgt ccattcacac gaatgatgtc
    11101 cccgtcgcgc acttttgcca gcagcccgcc atcgtaggct tctggtgtta cgtggatagc
    11161 tgacggcact ttacctgaag cgccggagag tcgtccatcg gtaactaacg caattttgaa
    11221 acaccggtcc aataatacac caagtggcgg catgagttta tgtaattctg gcattccgtt
    11281 cgcttttggc ccctgatgac ggacaacaac gacacaatcg cggtccagca aacccgcttc
    11341 aaaggccggc ataacgtcat gctggctttc aaaaacaacc gctggcgctt caatcacctg
    11401 gttctcaacc ggcacggcag aggttttcat aaccgcacgg cccaggttac cgcttaacac
    11461 ttttgtccca ccatgatgag agaaaggttg ttcgaaggaa gcgatcacat tgctgtcgag
    11521 tgatttttcc gccccttccc gccagtcctg ttcaccatta ttcagccatg gttcaagggt
    11581 ataacgagac agaccaaagc ctgccaccgt attgacatct tcatgcagca ggcctgcttt
    11641 gagcagttca cgcaccagaa ccggtacgcc acctgccgcc tggaagtggt taatatcggc
    11701 cggaccgttc gggtagagac gtgccatcag cggtacaaca tcagaaaggt cagagaagtc
    11761 atcccagtta atctgaatac cggccgcgcg cgccatcgcc accaggtgca tggtgtggtt
    11821 agtggaacca ccggtcgcca gcagtgcaac gataccgttc accaccactt tctcatcgat
    11881 catcttaccg atcggcatcc attcattacc attaccggtc atgcgtgtaa cctgacgcgc
    11941 agcggcagcg gtcaaagcat cgcgcagcgg agaatccgga tgaacaaaag aagagcctgg
    12001 caactgcatc cccataaatt ccaccaccat ctggttggtg ttggcagtac cgtagaaagt
    12061 acatgttccc ggcgcatggt aagacgcggc ttctgactcc agtaaggcca tgcggtccac
    12121 tttaccttcg gcataaagct ggcgaatacg cactttttct ttatttggca aaccgcttgc
    12181 catcggtcca gacggcacaa acaccgcagg caaatgacca agcgacaggg ctgccatcgt
    12241 cagacccggg acaatcttgt cgcacacacc gaggaacaga gcaccatcaa acatgttatg
    12301 ggacagcccc accgccgcag acatcgctat cacttcgcgg cttagcagcg acaattccat
    12361 tccatcctgc ccctgggtga caccatcaca catcgccgga acaccgcccg caacctgacc
    12421 aaccgcattc gcttcatgca gggctttacg aatgatttct ggatagtgtt cataaggctg
    12481 gtgcgcggag agcatgtcgt tataggaggt gatgatggcg atattgttac gcaacatgct
    12541 tttcaaagag gctttgtctt ctggctggca ggcagcgaaa ccgtgtgcca ggttaccgca
    12601 tgccaactgc gaacgatgaa cggtcgacgt tttcgcttgt tctatccggg cgagataagc
    12661 agagcgagtc tcgcgcgaac gttcaatgat tcgatttgtt acgcataaca attgtggatt
    12721 cataaaggct cctgaaattg agttgtcaga gcaggatgat tcacaacgcg tttcattcag
    12781 aggatttatg agtgaaacgc ctgtaaccgg agctcatagg gcaaaaacgt ttcagtcagt
    12841 gtaataaaaa aagcctcgtg ggtgaatccg cacgaggcct gaaagtgtaa aaattgttct
    12901 acaatctgtg caagatcatg ttaccggtaa aataaccata aaggataagc gcagatatta
    12961 ctcaaactca ttccaggaac gaccatcacg ggtaatcatc gccaccgagg caacgggtcc
    13021 ccaggttccg gcctgatacg gtttcggcgc atcattgtcc atcgcccacg cctcagtaat
    13081 ggagtctacc catttccagg cttcttccac ttcatcacga cgtacaaaca gtgcctgaat
    13141 accacgcatg gtttccagca gcagacgttc ataggcatcc gccagatgcg tctgattaaa
    13201 ggtttctgaa tagctcagat ccagcttggt gatttgcagg ttatgtttgt ggtcaaggcc
    13261 aggaacttta ttcagtacct ggatatccac gccttcatca ggttgcagac ggatagtcag
    13321 tttattctgc ggcagatcct gccacgactc tttaaacaga ttcagttcag gtgttttgaa
    13381 atagaccacg acttcagaac atttggtcgg cagacgttta ccagtacgca ggtagaatgg
    13441 cacaccggcc cagcgccagt tatcaatgtc gacgcggatc gccacgaagg tttctgtatt
    13501 gctgctcttg ttcgcgccct cttcttccag atatcccggc acttttttgc cctgggcgaa
    13561 gcccgcagta tattgcccgc gtacggtttt ttcgcgtacg ttggagcggt cgatgcggcg
    13621 cagggacttc agtactttca ctttttcatc gcggatgctg tctgcgctca ggtcagatgg
    13681 cggagacatc gcaatcatgc agagaatttg tagcaggtgg ttctgaatca tgtcacgcat
    13741 ctgaccggct ttatcaaaat agccccagcg cccttcgatc cccacttctt ctgccacggt
    13801 aatctcaaca tgatcaatgg tgcgattgtc ccagttattc acaaacaggg agttagcaaa
    13861 acgcagcgcc aacaggttca gcaccgtttc tttaccaaga tagtggtcga tacggtaaac
    13921 ctggcactcc tcgaagtatt cgccaacctg atcattgatt tcctgcgagg tcgccagcga
    13981 cgtccccagc ggtttctcca tgactacgcg tgccggttta gcattcagtt ttgcctcgcc
    14041 aagccctttg caaattgcgc caaaagtgct gggcggcatg gcaaagtagt taatggtgat
    14101 acgatttttt tgatccagca tcgcgccgag acggttgaat gcagcagtgt cattgacatc
    14161 gagattacaa aaatccagac gtgcactcag ggtgtcccat aaaccttcat caatggtttc
    14221 tttcatgaaa gtttcgagcg cctcgcggac aactttggta tatgccgctt tatcccagtc
    14281 agcacgccct acgccgataa tccgggtgtc cgggttgagc tgaccggctt tttccagttg
    14341 atacagggaa ggcagcaatt tacgacgcgc aaggtcgcct ttcgcgccga aaatgaccag
    14401 gtcacaggcc tgggctgttt gcgttaccgc catgtcattc tccttaagtt aactaacccg
    14461 gtacttaagc cagggtatac ttgtaatttt cttacggtgc actgtactgc ttttacgagc
    14521 ttgcgaaaac tgtaaacgct tatccacccg tgcgattacg ggaaaagcgc gcaaagtgcg
    14581 gcaaaactga taaaaaaatc atcgttttcc attcggtaaa aatctgacac tgatcatgtt
    14641 atgaaaaaaa ataacaactt ttttatctgc ttttgtcatt aacacggcac acaggcgtaa
    14701 tatctgacaa aactgcattt cgatttcttt cagtgcggaa atcgtcatta cccgtgagtc
    14761 tctttacatc atgaatatgc tggaaaaaat ccagtctcag ctggaacatt tgagcaaatc
    14821 agagcgcaaa gttgccgagg tcattctggc ttcgcccgat aacgcgatcc attcgagtat
    14881 tgctgctatg gcactggaag ccaatgttag cgaaccgacg gtgaatcgtt tctgtcgcag
    14941 catggacacg cgcggttttc ctgattttaa acttcatctg gcacagagtc tggcgaatgg
    15001 cactccctat gttaatcgca atgtcaatga agatgacagc gttgaatcat acacagggaa
    15061 aatatttgag tccgcaatgg caacgcttga tcatgtccgt cattcactgg ataaatctgc
    15121 catcaaccgc accgtcgact tgctcactca ggcaaaaaaa atcgcctttt tcggattagg
    15181 ctcttcagcc gccgttgccc acgatgcgat gaataagttc tttcgtttta atgttccggt
    15241 ggtgtactcc gatgatatcg tgctgcaacg catgagttgt atgaattgta gcgacggaga
    15301 cgtggtggtg ctgatttctc acactggaag aacaaaaaat ctggtcgagc tggcgcagct
    15361 ggctcgcgaa aacgacgcaa tggtgattgc cctcacctct gcgggtaccc cgctcgcccg
    15421 ggaagcaacg ctggcaatta ccctcgacgt accggaagat actgacattt atatgcccat
    15481 ggtttctcga cttgcacagc tgaccgtgat agatgtgctg gcgacaggat ttactttgcg
    15541 acgcggtgca aaattcagag ataacttgaa gcgggtcaag gaagcgctga aggaatcgcg
    15601 ttttgataag cagttactta atttaagtga cgatcgctaa aaacgactgt cactgtccta
    15661 atcttatacg acatccgaat gagattaatt tatcgccatc gcggcgttat ttcattcgga
    15721 tttcatgttc aagcaacacc tggttgtttc agtcaacgga gtattacatg tccagaaggc
    15781 ttcgcagaac aaaaatcgtt accacgttag gcccagcaac agatcgcgat aataatcttg
    15841 aaaaagttat cgcggcgggt gccaacgttg tacgtatgaa cttttctcac ggctcgcctg
    15901 aagatcacaa aatgcgcgcg gataaagttc gtgagattgc cgcaaaactg gggcgtcatg
    15961 tggctattct gggtgacctc caggggccca aaatccgtgt atccaccttt aaagaaggca
    16021 aagttttcct caatattggg gataaattcc tgctcgacgc caacctgggt aaaggtgaag
    16081 gcgacaaaga aaaagtcggt atcgactaca aaggcctgcc tgctgacgtc gtgcctggtg
    16141 acatcctgct gctggacgat ggtcgcgtcc agttaaaagt actggaagtt cagggcatga
    16201 aagtgttcac cgaagtcacc gtcggtggtc ccctctccaa caataaaggt atcaacaaac
    16261 ttggcggcgg tttgtcggct gaagcgctga ccgaaaaaga caaagcagac attaagactg
    16321 cggcgttgat tggcgtagat tacctggctg tctccttccc acgctgtggc gaagatctga
    16381 actatgcccg tcgcctggca cgcgatgcag gatgtgatgc gaaaattgtt gccaaggttg
    16441 aacgtgcgga agccgtttgc agccaggatg caatggatga catcatcctc gcctctgacg
    16501 tggtaatggt tgcacgtggc gacctcggtg tggaaattgg cgacccggaa ctggtcggca
    16561 ttcagaaagc gttgatccgt cgtgcgcgtc agctaaaccg agcggtaatc acggcgaccc
    16621 agatgatgga gtcaatgatt actaacccga tgccgacgcg tgcagaagtc atggacgtag
    16681 caaacgccgt tctggatggt actgacgctg tgatgctgtc tgcagaaact gccgcagggc
    16741 agtatccgtc agaaactgtt gcagccatgg cgcgcgtttg cctgggtgcg gaaaaaatcc
    16801 cgagcatcaa cgtttctaaa caccgtctgg acgttcagtt cgacaatgtg gaagaagcta
    16861 ttgccatgtc agcaatgtac gcagctaacc acctgaaagg cgttacggcg attattacca
    16921 tgaccgaatc gggtcgtacc gcgctgatga cctcccgtat cagctctggt ctaccaattt
    16981 tcgccatgtc gcgccatgaa cgtacgctga acctgactgc tctctatcgt ggcgttacgc
    17041 cggtgcactt tgatagcgct aatgacggcg tagcagctgc cagcgaagcg gttaatctgc
    17101 tgcgcgataa aggttacttg atgtctggtg acctggtgat tgtcacccag ggcgacgtga
    17161 tgagtaccgt gggttctact aataccacgc gtattttaac ggtagagtaa tctcacctgc
    17221 cccggccgct gatggccggg gtatttagca acacataaat taggactact tattcaatcc
    17281 aatcataatc acagccccct gtaaaatgaa tacagggata gcctgaatgg agatctaaat
    17341 agcttgcaag acgtcgcata cccgcttcct ctgcaacact atgtccaata actgccatcg
    17401 gtaagccact atctttggag tattgtgtct gcacccatgt tttaattgaa tcgttaatcg
    17461 taatataata atcagcctga tattccatat attgtattgg atcacaaaaa cagcctgccc
    17521 caatgccaac agttctgact actctggctt tatctccata aaattgaatg ccaggaagat
    17581 tatatatttt caaatttttg gcaaaacgtt tggtaatgtt aatggcactg tcagggtcta
    17641 tagcatatac acagtacatc tcatcagaag caacaatatt tgtaacatcc agttctaact
    17701 gctgagccaa agccttagac attccgaacg taggctctcg atccatgaca tcatggcaac
    17761 gaatgattac caaattattt ttatgcaaaa actccttttt ttgctcaatt atttttaaat
    17821 aactgagttc accgcgagta taatccatta tttttctggc ccatgaaaac tcaagatcgt
    17881 caccctccag atcagcataa gaataaaatg taggctcatg tgtaataatg atattgacac
    17941 cttcaaaaac agcctttctt aaaacatcca ttgaggcaat ccagcaggtc ccaattttat
    18001 taataagtgt cgcctcagca ccaaaaataa ttttatcaac agttggttgc gaaacagcaa
    18061 ccattgaatt taaaaaacaa ttaagatccg cggctgtttt aatatgatgc attcaacatc
    18121 cttcacaggt ttcatcagca ataaaataat ttcaacattt ctgggaaacg ttttgcccat
    18181 gcagactcag agtgggtatc acctccccat aaat
//
LOCUS       DAEEKQ010000075        17957 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.075, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000075 DAEEKQ010000000
VERSION     DAEEKQ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17957
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.075"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(227..1201)
                     /gene="cysB"
                     /locus_tag="KLK57_002423"
     CDS             complement(227..1201)
                     /gene="cysB"
                     /locus_tag="KLK57_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HBE0892773.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFTIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(1411..4008)
                     /gene="topA"
                     /locus_tag="KLK57_002424"
     CDS             complement(1411..4008)
                     /gene="topA"
                     /locus_tag="KLK57_002424"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HBE0892774.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTIGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKIGEIVTDRLEENFRELMNYDFTAQMENSLDLVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            4388..4639
                     /locus_tag="KLK57_002425"
     CDS             4388..4639
                     /locus_tag="KLK57_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HBE0892775.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FIGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="KLK57_002426"
     CDS             complement(4675..5724)
                     /gene="sohB"
                     /locus_tag="KLK57_002426"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HBE0892776.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            5944..6702
                     /locus_tag="KLK57_002427"
     CDS             5944..6702
                     /locus_tag="KLK57_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HBE0892777.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            6699..7289
                     /gene="cobO"
                     /locus_tag="KLK57_002428"
     CDS             6699..7289
                     /gene="cobO"
                     /locus_tag="KLK57_002428"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HBE0892778.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="KLK57_002429"
     CDS             complement(7329..8201)
                     /gene="rluB"
                     /locus_tag="KLK57_002429"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HBE0892779.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(8414..9528)
                     /locus_tag="KLK57_002430"
                     /pseudo
     CDS             complement(8414..9528)
                     /locus_tag="KLK57_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
     gene            complement(9556..10176)
                     /locus_tag="KLK57_002431"
     CDS             complement(9556..10176)
                     /locus_tag="KLK57_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HBE0892780.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(10173..11054)
                     /gene="yciV"
                     /locus_tag="KLK57_002432"
     CDS             complement(10173..11054)
                     /gene="yciV"
                     /locus_tag="KLK57_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HBE0892781.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEVLVHRAVEMRVGTLAITD
                     HDNTAAIVPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGARRLAQGGAVTRGHFARFLVGCGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            11192..11236
                     /gene="trpL"
                     /locus_tag="KLK57_002433"
     CDS             11192..11236
                     /gene="trpL"
                     /locus_tag="KLK57_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HBE0892782.1"
                     /translation="MKAIFVLKAWWRTS"
     misc_feature    11213..11306
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            11328..12890
                     /gene="trpE"
                     /locus_tag="KLK57_002434"
     CDS             11328..12890
                     /gene="trpE"
                     /locus_tag="KLK57_002434"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HBE0892783.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVENEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKNTRIQASLFAPNEEEKQRLTARLNDLRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSSYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSIPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            12890..14485
                     /gene="trpD"
                     /locus_tag="KLK57_002435"
     CDS             12890..14485
                     /gene="trpD"
                     /locus_tag="KLK57_002435"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HBE0892784.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPILMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            14486..15847
                     /gene="trpCF"
                     /locus_tag="KLK57_002436"
     CDS             14486..15847
                     /gene="trpCF"
                     /locus_tag="KLK57_002436"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HBE0892785.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIASIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLILVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPARELQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            15859..17052
                     /gene="trpB"
                     /locus_tag="KLK57_002437"
     CDS             15859..17052
                     /gene="trpB"
                     /locus_tag="KLK57_002437"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HBE0892786.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPEKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            17052..17858
                     /gene="trpA"
                     /locus_tag="KLK57_002438"
     CDS             17052..17858
                     /gene="trpA"
                     /locus_tag="KLK57_002438"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HBE0892787.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISTPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKVATRS"
BASE COUNT         4315 a         4749 c         4488 g         4405 t
ORIGIN      
        1 cagcgaattt acgaatcgtc tccagtgaat aattcaatct tttgacctga aatagatatt
       61 tgaaaactgg agtttgcgag gcgggtaatt agacactact ttacaggtaa tttaccctat
      121 aaatatgagg gataaaaata ttgtgcacgg tatagaaata agtaacaaaa gagaatatat
      181 tccggcacct tcgctacata aaaggtgccg aaaataacgc aagaaattat ttttccggca
      241 gttttatatc tttaaacatg acctcaattt cttcattaga gcgcaatgcg acagccgcat
      301 caacgacatc acgcgttaaa tgcggtgcaa aacgctgaat gaaatcatac atataactgc
      361 gcaagaaagt actacggcga aaaccaattt tggttgtact gtggctgaag atatcgtgag
      421 catcaacacg cacaaggtcg ggatcggcga ccggatccac cgccatgctg gcaatgaccc
      481 ctacccccag ccctaaccgg acgtaagttt taatgacgtc agcatccgtt gccgtgaaga
      541 cgatacgcgg cgttaaccct gcgcggttaa aggcagtatc cagttctgaa cgtccggtaa
      601 agccgaaggt atatgtcacc aacggatatt gcgccagttc ttcaatggta atggcttttt
      661 tgcctgccag cgggtgatcc ggagtgacta caatagcccg attccagtgg tagcacggta
      721 acatcactaa atcttcatac agatgcagcg cttctgtggc gatagtgaaa tcagcattgc
      781 ctttagagac ggcatcagca atttgtgtcg gcgagccctg gtgcatatgc aaagaaacgc
      841 ggggataacg ctcaataaag cctttgataa cgtttggtaa cgcgtagcgt gcctgggtat
      901 gcgtggtggc gatatacagt gagcctttat ccggccaggt gtgctctccg gcaaccgatt
      961 ttatggcatc gactttcgac aggacttcgc gagcgatacg aattatttct tgccctgctg
     1021 gcgttacctg cgtcaggtgc ttgccgcttc gggaaaaaat ttgaatgcct agctcgtctt
     1081 ccagcattct gacttgttta ctgatcccgg gttgtgatgt gtaaagtcct tccgctgttg
     1141 atgagacatt caggttatga ttgaccacct caacaatata gcgaagttgt tgtaatttca
     1201 tgttaaacca tccacttaga cgccatcaga acatcgtttt ttcgtcacac ctggcgggtg
     1261 actaatacga tttaataata aaaaaggtgc taactataac cactatatca tttatagcct
     1321 tagtgtatag gtgtgaagaa aaaataatat gcaataaaaa agggccgctt tcgcgaccct
     1381 ttgtttataa aaacctgaca gaattaaagg ttattttttt ccttcaaccc atttgccatc
     1441 aacataaaat gctgaccagc cagtcgcctt tccgtctttt tccgaagaga catattgctg
     1501 tttggtttta cggctaaagc gaaccatggt cttattacct tccggatcct gctgtggcgc
     1561 atcggccaga taacgcagtt tttccggcag acggtcgcgg aagcgataaa gctcttccac
     1621 cagtggcgca cgcgtttcac gcgatttcgg gaaagtgttg gcagccagga aaacacccgc
     1681 agcgccgtca cgcagcacga aataagcatc tgatttttcg cacggcagct caggtaatgg
     1741 caccggatct tctttcggtg gtgccacttc gccgttacgt aaaatcttac gtgtgttttt
     1801 acactcttcg ttggtgcagg ccatgtattt accgaaacgc cccattttca ggtgcatttc
     1861 agagccacat ttttcacact caacgatcgg gccgtcataa cctttaatgc ggaattcgcc
     1921 ctcttcgatc tcgtaaccgt cgcaggttgg gttattacca cagacatgca acttacgttt
     1981 cggatcgatg agatagctgt ccatcgccgt gccgcatttc gggcaacgac gttttgcgcg
     2041 cagcgcgttg gtttcagcgt cttcgccttc cagtacgttc agcacttcgt tttccggcac
     2101 caggttaatg gtggttttgc aacgctcttt cggcggcagc gcataaccag aacagccaag
     2161 gaataccccg gtgctcgctg tgcgaatccc cattttgcga ccacaagtcg ggcagtcaat
     2221 gctggtcaga accatctggt tcgggcgcat accaccctct tccggatctt tttcagcttt
     2281 atctaactgc tgggtgaaat ccgagaagaa gtgatccagt acagctttcc actctgcttc
     2341 gtgatttgcc accaggtcga ggctgttttc catctgtgcg gtgaagtcgt agttcattaa
     2401 ctcgcggaaa ttttcttcaa ggcgatcggt gacgatttcg ccaatttttt ccgcatagaa
     2461 acgacgattt tctactcgca cgtagccacg atcctgaatg gtcgaaatga tcgacgcata
     2521 ggtagacgga cgaccgatac cgcgtttttc cagctcttta accagcgatg cttcactgaa
     2581 acgggctggc ggcttggtaa agtgctgggc tggtgtaagt tcaacgagcg tcagagcatc
     2641 gcctttatta actgctggta agatgcgatc ttcatcgcct ttacgcaacg caggcatcac
     2701 ttttgtccag ccatcaaaac gcaaaatacg accgcgtgct ttcaggcgga aatcgcccgc
     2761 accaatggtc agcgtcgtgg agtcatattt cgctggggtc atctggcagg ctacgaactg
     2821 acgccagatt aactggtaca gtttctgcgc atctgcttcc atatccttca gcgattccgc
     2881 catcacattg acgtcagaag gacgaatcgc ttcgtgcgct tcctgtgagt tttctttgct
     2941 ggcgtactga ttcggacttt ccggcagata tttcttacca aaattatcgc tgatataacc
     3001 acgaaccata tttaccgcgt cctgactcag gttagtggag tcggtacgca tgtaagtgat
     3061 atagcctgct tcatacaaac gctgcgccat catcatggtt tttttcacgc caaatccaag
     3121 acgggtgctg gcagcttgtt gcagcgtaga ggtaataaaa ggagcgccag gtttactggt
     3181 tgtcggtttg tcttcacgtt ccagcacgct gtagcgcgct ttttccagca gacttaccgc
     3241 agcctgagtt tgttctttgt tgaccggacg gaacggtttg tcgttctgat gagtcacctg
     3301 taacgccaac gcttcaccag atggcgtggt cgtgctggca tcgacttccc agaactcttc
     3361 cggcacgaac gctttaattt cacgctcacg ctcgaccacc aggcgaaccg ccaccgactg
     3421 cacacgaccg gcagacagac cacgagcgat ctttttccat agcagcggcg aaaccatata
     3481 ccccaccacg cggtccataa agcgacgcgc ctgctgggca ttaacacgat caatattcag
     3541 ctcacccggt ttgttaaatg cctggcggat cgcgttttta gtaatttcgt taaacaccac
     3601 tcggctatag cgcgcatcat cacccccaat cacttcccgc aggtgccatg caatggcttc
     3661 cccttcgcgg tcaaggtcgg ttgcgagata gatgtggtcg gctttttcag ccagttgttt
     3721 cagttcagag acgaccttct ctttaccagg caacacttca tagtgcgcct cccaattgtg
     3781 ccacgggtca acccccatac ggttgacgag agcgccacgt tcatcctttt taggcttttt
     3841 agccgtcttg gtggaggtag agtcggcact ctttttggca gctgagccac tggtcggcaa
     3901 atcgcggatg tgaccgacgc tggatttcac cacgtagtca ctacccagat acttgttgat
     3961 cgttttggct tttgccgggg actcaacgat gacaagagct ttacccatat tcaccttacc
     4021 taatttgatt cttccaggaa tgcgtcgcac gttgataaac cttccactgg caacgagtta
     4081 ccgatatgga ctcgttctct gcggatatca accccttgtc gtgactctgt tcacgaaaat
     4141 tcaggtgact gagttaacag gtcttggcct acaggcgcta tagcacgacg gaattttggt
     4201 cgaatgtcaa gcaaatctat tgccagattg acgaaagcgt cacactgtac ctgataaaat
     4261 ccgatacgca actttattag catgcaaaat ccaaagttgc cagtaaggcg ctcttgccac
     4321 cagcgtggta tatttgtctg caagccatgc ctggcgtaga ctaatgtcct tgtttaagga
     4381 gtaaataatg aacaaagaga cacaacccat cgaccgcgaa acgctgttga aagaggccaa
     4441 caaaatcatt cgtgagcatg aggatacgct cgcagggatt gaagccaccg gcgtcacgca
     4501 gcgtaatggc gtgctggtat ttatcggcga ctacttttta gatgaacaag gattgccaac
     4561 agccaaaagc accgcggtgt ttaatatgtt caaacacctg gcccatgtgc tttcagaaaa
     4621 atatcatctg gttgattaaa gcaaaacgcg aggtcttagc ctcgcgtttg tcttttacat
     4681 caatggcttt tgaccccgct gccaccagcg tagcaacaat cgatcggcgc tctctgccgc
     4741 gctgccggtg aatcggtcaa tgagtcgttt acgctgcata tagcgtacat taaccacttc
     4801 acggccttcc atcaggctaa gaataacttc atcactggtg ttgatttcat caaccaggcc
     4861 tttctctacc gcctgttgtc cgtaccagtg ttcacccgtt gccacctgtt caatatccag
     4921 agacggacgc atacgcttca caaaatcttt aaataactga tgcgtttcgt tcagctcttc
     4981 gcggaatttc tcccgccctt cttcggtatt ttcacccagc aaggtcagcg tgcgcttata
     5041 ctgcccggcg gtgtgcagtt cgatatcaat atctttgctt ttcaggaagc ggttaaagtt
     5101 gggcatttgc gccaccaccc caatggaacc cacaatagca aacggtgcgg aaacaatttt
     5161 gtccgccaca caggccatca tgtaaccgcc gctggcagcg actttgtcta ccgtaacagt
     5221 taaaggaatg tttttatcac gcagacgctg cagctgcgaa gccgccaacc cgtaaccatg
     5281 caccatgcca ccagggcttt ccagacggag cacaacctga tcctgcggtt tgaatgctgc
     5341 gagtaccgcc gttatctctt cacgtagcga gttcacttca tgggcgtcca tgctgccttt
     5401 aaaatccagc acccagacgc gaggtttact gtcagttgcc acctcgccca gtttggcttt
     5461 cgcttttgct gctttcgctt cttgcttgtg ctttttcttc tgcgctttgt gccactgttt
     5521 ttgctgatgt gagtccatca gcgccgcggc cagttcttct ttcatctcct tatactgttc
     5581 gctgagattg ttgacccgta actcgccacg ctggcgttta ttacgttgag caacattgac
     5641 aataatggcg gcaatcgccg caatcgctag cacaacggta acgattttcg ccaaaaacaa
     5701 accatattca gacaacaatt ccacgagtcc ccaccttggt taaacaacgc tttggctcac
     5761 agtttacaac aggcatgtta agccgtctcg cagagatgtt attgcagcga aagtatgaca
     5821 aagtatccat tttgcggtgt tatcgcccca ggctgattga aatgcccttc tgtttcaggc
     5881 ataaagcccc aaagtcataa agtacactgg cagcgcgaca gacaacgtcg ttgaggagta
     5941 gccatgcatt accagccaaa acaagattta ctcaatgatc gcattatcct ggtgacggga
     6001 gccagcgatg ggattggtcg tgaagccgcg atgacgtatg cacgctatgg tgcgacagtg
     6061 attctgttgg gccgtaatga agaaaaatta cgtcaggtag ccagccacat aaacgaagaa
     6121 actgggcgtc agccacagtg gtttattctc gatttgctga cctgcacgtc cgaaaattgc
     6181 caacaactgg cacagcgcat tgtcgttaat tatccgcgtc tggatggtgt tttgcataat
     6241 gccggattgc tcggcgatgt ttgcccaatg agcgaacaaa atccgcaggt ctggcaggac
     6301 gtcatgcagg tcaacgttaa tgccaccttt atgctcaccc aggcactgct tcctttatta
     6361 ctcaaatcgg acgccggttc actggtcttt acttcatcaa gcgttgggcg tcagggacga
     6421 gccaactggg gtgcatatgc agcgtcgaaa tttgccaccg aagggatgat gcaggtactg
     6481 gccgatgaat atcagcagcg cctgcgcgtc aactgcatta acccaggcgg tacgcgcacc
     6541 gcaatgcgcg caagcgcctt cccgaccgaa gatccacaga aacttaaaac acccgctgat
     6601 atcatgccgc tctacctctg gctgatgggc gatgacagcc gccgtaaaac cggcatgacc
     6661 tttgacgccc aaccgggccg taaaccagga atttcccaat gagtgatgaa cgctaccaac
     6721 agcgtcagca gcgagtgaaa gaaaaagtag atgctcgtgt ggcccaggcc caggatgaac
     6781 gcggtattat catcgtcttt accggcaatg gaaaaggcaa aaccaccgcg gcatttggta
     6841 cggcaacacg cgcagttggt cacggaaaaa aagtgggcgt cgtgcagttt attaaaggca
     6901 cctggcctaa tggcgaacgc aatctgctgg agccacatgg cgttgagttt caggtgatgg
     6961 caacgggctt tacctgggat acacaaaacc gcgagtctga taccgccgcc tgccgcgaag
     7021 tctggcaaca tgcaaagcgg atgcttgctg attcctcact ggatatggtt ttgcttgatg
     7081 aactgacgta tatggtggcg tatgactatt tgccactgga agaagtggtg caggcgttaa
     7141 atgaacgtcc acatcaacag acggtgatta tcacgggtcg aggctgtcat cgggatattc
     7201 ttgaactggc ggatacggta agtgagctgc gccccatcaa acatgcgttt gatgccggtg
     7261 ttaaggcgca aatagggatt gattattaat ctgtaaaaat gccggatatg acgtccggca
     7321 tactttgatt aaccgttatt acgcccgcca gaacgacgac cgccgctcac ctgactgtga
     7381 cgtttcaccg cacgacgaat ctgattagcc ttcatgcgac gacggtcttt ttctacagcg
     7441 actttagagc tggtttctgg cggaagctcc accagttcgc gcagatagtt agtctgggcg
     7501 aggtccagct ccgtccagcc accgcgtggc agaccttttg gcagtgggat atcaccgtaa
     7561 cgaacacgga tcaggcggct cacctgcaca ccaaccgctt cccacagacg acgaacttca
     7621 cggttacggc cttcggtcag agtcacgttg taccactggt tgatcccttc gccgccgctg
     7681 aacttgatgg ttttaaaagc tgccggacca tcttccagtt gcacgccacg actcaaatca
     7741 cgcagtttcg cgtcgtcaac ctgaccaaat acacgcacgg cgtattcacg ttcaacttca
     7801 cggcttggat gcattaaacg gtttgccagt tcaccatcgg tggtgaacag cagcagacca
     7861 caggtgttaa cgtccagacg acctacggca atccagcgtg cgccacgcag ctttggcaga
     7921 cggtcaaaca ccgtcggacg cccttccgga tcgttacggg tacacaactc accttccggt
     7981 ttgtaatagg ccagaacgcg acaaatttgt tcagcggact cacgtaccga aatcagatga
     8041 ccatcgatac gaattttcag cccaggggta acttcaacgc gatcgccgag tttagcaatt
     8101 ttgccatcaa cactcacacg accggcttca ataatagatt cgatttcacg gcgggagccg
     8161 tggccagcgc gggccagcac tttctgtaat ttttcgctca tagagcttcc ttttggtgtc
     8221 gccttcccag gcgtctgata tgcttttgtg caacagtgag tcagaactta catctgactc
     8281 actgatagta aagtctgttt gaactcttca ggccttaagt aaacgactcg acgttttaac
     8341 tggcctggct ttgaggcgca acatactaca ctatctttat ggttaaagct tgtttcgatt
     8401 aacatcagct taattaccag ccaccaccgc ctccgccacc cgcgccgcca ccagcaccac
     8461 caccggaata acttcgcccc aacaaactgg ttttggcccc cagactcagg tgcatctgta
     8521 atgaaaacaa aacgccacta cgggcaattt ccggggcgct aacagccgct gtgagtgatg
     8581 gggtgatacg tgctacccat tctcttccaa ggtttgctga gactgcccaa cctagcaacg
     8641 cgtaatccag tttccgtgtt tcccctttcc gccgacgccc actgtgtgtt gctgcctctt
     8701 tacgcgcaag atagttaacg atagcttcac cgtgggcata acgttgctgc ccagtttgtg
     8761 taaactttgg cagaaaaata taccccatgc cagtgagata accggtcagg aaaacagcgc
     8821 taaaataacc cgctggcata taccagtaaa agaatacatc tccgacattg atgtagagag
     8881 aatagaaggc cacgcctgaa caaatcagtg gtaaacaatt cttccgtcct caaggatgcg
     8941 gccatttcga tatgtgtcgc cctcttctcc agtaaagaga tcgatttcgc taaatggatt
     9001 acccgcgata tcagggaact tgagtgaaaa tcgggtctta tagatttcaa acggccagtg
     9061 gttaccagtc acgttccaga ttaacagatc tgaatctccc tcacgcagga aagcattttt
     9121 aacctgataa tgaatttgat aatgatagtc gccttgaggc aagaaacgtt gtttatcgcc
     9181 cacgacaata ctcattaacc cgatatgcca gtcatgatgc cagggttcag gaataccatc
     9241 acgggtgacg ccaacaatat gatagttcag cagtgcagcg tccccaccag gctgcatcca
     9301 gagtcgtggt aaatcacgat aaaatccgtg tttaatctgc tgatttcgcg cctgaaaatg
     9361 aatattttct ttgatgtcga gcaccccatc cggttgcaga gtggcttcaa catcaaagag
     9421 cgaaatccct tcttcataag aagggatctc cgtgttttct gcataactta gagatgatac
     9481 aaatagccct gctatcaaca gcaaaatgca gcgaaacttc cctgccatag tcttccatcc
     9541 gtggaacgca atttgttata agaaaggctt cacatcacct acgccttcac gcaccacgac
     9601 cggcgtatca tcggtaagat caataaccgt tgtcggtttc tgcccgagat agccgccgtg
     9661 aataatcaaa tctacctgtt tttccagacg gtctttaatt tcttcgggat cggattcggt
     9721 aaattcgctg cctggcagca ttagcgaagt ggaaagcatc ggttcgccca gtgcttcaag
     9781 caacgcctgc gcaatcgggt tagaaggcac acgcataccg atggttttgc gtttttcctg
     9841 caacagacgg cgtggcactt ccttcgtccc cttcaggatg aaggtatagt tgcccggcgt
     9901 gttgtttttc attaaacgaa acgcaacgtt atcaacaaat gaataggtcg agagttcaga
     9961 aagatcgcga cacatcaggg taaagttgtg accgtccggc agctggcgaa tacgacaaat
    10021 acgctccatc gcgtttttat cttcaatttt acagccgagc gcatagccgg aatcagttgg
    10081 ataaacaatc accccgcctt tacgcacgat ctccaccgcc tggttgatca gacgttgctg
    10141 tgggttatca ggatgaatat aaaaaaactg gctcataatt ccctctctgt ggtgttctgc
    10201 ggctgttccc atagctgcca gacgccttca acgcctgcgg gcagccagag tttacgacct
    10261 agttcaatcc acgggcatgg ctgatgaaaa tcagatcctt gtgatgccca taaatgatgc
    10321 tgacgcgcaa gggccgccag ctgggtacgt tcattgggcg attgctggca ctgcgcgact
    10381 tccatcgcgt caccgtggtg ttcggcaaaa tgcgcaacca atcttttcag ccatttagca
    10441 ctaagattgt accgcccagg atgagctagc accgccttac cgccagaatg atgaatgaca
    10501 tcaatagctt gttctattgt acaccactgt ggcggaacgt atccggtttt cccgcgcgcc
    10561 agatattttt taaagacatc cgccattgaa ctggctttgc cgcaccccac aaggaaacgc
    10621 gcaaaatgac cgcgcgtcac cgcaccgccc tgcgccagtc gtcgcgcgcc ttccagtgcc
    10681 cctggtattt gcgctttttc aagtctttcg gcaatcagtt gcgcccgctg attgcgccgt
    10741 tctgtctgct gcgcgaggaa ctcacacatt agcggatgag taatatcaat attcagcccg
    10801 acaatatgaa tttcatgatt ttcccagacc gtggaaattt ccacgccggg aataagattc
    10861 agcgccagtc ccgaacgtga aatttcctct cttgctggca cgattgcagc tgtgttgtca
    10921 tggtcggtga tcgccagggt accgacgcgc atctcgactg cacggtgcac caatacttct
    10981 ggcgtcaggc agccatcgga agctgtggta tggctgtgca ggtcgtaaat cactgcgtaa
    11041 ttcgtgtcgc tcaaggcgca ctcccgttct ggataatgtt ttttgcgccg acatcataac
    11101 agttctggca aatattctga aatcagctgt tgacaattaa tcatcgaact agttaactag
    11161 tgcgcaagtt cacgtaaaaa gggtatcgac aatgaaagca attttcgtac tgaaagcttg
    11221 gtggcgcact tcctgaaacg ggcagtgtat tcaccatgcg taaagcaatc agatacccag
    11281 cccgcctaat gagcgggctt ttttttgaac aaaattagag aataacaatg caaacacaaa
    11341 aaccgactct cgaactgcta acctgcgaag gcgcttatcg cgacaacccg actgcgcttt
    11401 ttcaccagtt gtgtgggaat cgtccggcaa cgctgctgct ggaatccgca gatatcgaca
    11461 gcaaagatga tttaaaaagc ctgctactgg tagacagtgc gctgcgcatt acagcattag
    11521 gtgacactgt cacaattcag gcactttccg gcaacggcga agccctgctg gcactactgg
    11581 ataacgccct gcctgcgggt gtggaaaatg aacaatcacc aaactgccgt gtgctgcgct
    11641 tcccccctgt cagtccactg ctggatgaag acgcccgctt atgctccctt tcggtttttg
    11701 acgctttccg tttattgcag aatctgttga atgtaccgaa ggaagaacga gaagcaatgt
    11761 tcttcggcgg cctgttctct tatgaccttg tggcgggatt tgaagattta ccgcaactgt
    11821 cagcggaaaa taactgccct gatttctgtt tttatctcgc tgaaacgctg atggtgattg
    11881 accatcagaa aaaaaacacc cgcattcagg ccagcctgtt tgctccgaat gaagaagaaa
    11941 aacaacgtct cactgctcgc ctgaacgatc ttcgccagca gctgaccgaa gccgcgccgc
    12001 cgctgccggt ggtttccgtg ccgcatatgc gttgtgaatg taatcagagc gatgaagagt
    12061 tcggtggcgt agtgcgtttg ttgcaaaaag cgattcgcgc tggagaaatt ttccaggtgg
    12121 tgccatctcg ccgtttctct ctgccctgcc cgtcaccgct ggcggcctat tacgtgctga
    12181 aaaagagtaa tcccagcccg tacatgtttt ttatgcagga taatgatttc accctgtttg
    12241 gcgcgtcgcc ggaaagttcg ctcaagtatg acgccaccag ccgccagatt gagatctacc
    12301 cgattgccgg aacacgtcca cgcggtcgtc gtgccgatgg ctcgctggac agagacctcg
    12361 acagccgcat cgaactggaa atgcgtaccg atcataaaga gctttctgaa catctgatgc
    12421 tggtggatct cgcccgtaat gatctggcac gcatttgcac ccccggcagc agctacgtcg
    12481 ccgatcttac caaagttgac cgttactctt acgtgatgca cctggtctcc cgcgtggtcg
    12541 gtgagctgcg ccacgatctc gacgccctgc acgcttaccg cgcctgtatg aatatgggga
    12601 cgttaagcgg tgcgccgaaa gtacgcgcca tgcagttaat tgccgaggcg gaaggtcgtc
    12661 gccgcggcag ctacggcggc gcggtaggtt atttcaccgc gcatggcgat ctcgacacct
    12721 gcattgtgat ccgctcggcg ctggtggaaa acggtatcgc caccgtgcaa gccggtgctg
    12781 gcgtagtcct tgattctatt ccgcagtcgg aagccgacga aacccgtaat aaagcccgcg
    12841 ctgtactgcg cgctattgcc accgcgcatc atgcacagga gactttctga tggctgacat
    12901 tctgctgctc gataatatcg actcttttac gtacaacctg gcagatcagt tgcgcagcaa
    12961 tgggcataat gtggtgattt accgcaacca tattccggcg cagaccttaa ttgaacgcct
    13021 ggcgacgatg agcaatccga tactgatgct ttctcctggc cccggtgtgc cgagcgaagc
    13081 cggttgtatg ccggaactcc tcacccgctt gcgtggcaag ctgcccatta ttggcatttg
    13141 cctcggacat caggcaattg tcgaagctta cgggggctat gtcggtcagg cgggcgaaat
    13201 tcttcacggt aaagcgtcga gcattgaaca tgacggtcag gcgatgtttg ccggattaac
    13261 aaacccgctg ccggtggcgc gttatcactc gctggttggc agtaacattc cggccggttt
    13321 aaccatcaac gcccatttta atggcatggt gatggcagta cgtcacgatg cggatcgcgt
    13381 ttgtggattc cagttccatc cggaatccat tctcaccacc cagggcgctc gcctgctgga
    13441 acaaacgctg gcctgggcgc agcagaaact agagccagcc aacacgctgc aaccgattct
    13501 ggaaaaactg tatcaggcgc agacgcttag ccaacaagaa agccaccagc tgttttcagc
    13561 ggtggtgcgt ggcgagctga agccggaaca actggcggcg gcgctggtga gcatgaaaat
    13621 tcgcggtgag cacccgaacg agatcgccgg ggcagcaacc gcgctactgg aaaacgcagc
    13681 gccgttcccg cgcccagatt atctgtttgc tgatatcgtc ggtactggcg gtgacggcag
    13741 caacagtatc aatatttcta ccgccagtgc gtttgtcgcc gctgcctgtg ggctgaaagt
    13801 ggcgaaacac ggcaaccgta gcgtctccag taaatctggt tcgtccgatc tgctggcggc
    13861 gttcggtatt aatcttgata tgaacgccga taaatcgcgc caggcgctgg atgagttagg
    13921 tgtttgtttc ctctttgcgc cgaagtatca caccggattc cgccacgcga tgccggttcg
    13981 ccagcaactg aaaacccgca ccctgttcaa tgtgctgggg ccattgatta acccggcgca
    14041 tccgccgctg gcgttaattg gtgtttatag tccggaactg gtgctgccga ttgccgaaac
    14101 cttgcgcgtg ctggggtatc aacgcgcggc ggtggtgcac agcggcggga tggatgaagt
    14161 ttcattacac gcgccgacaa tcgttgccga gctgcatgac ggcgaaatta agagctatca
    14221 attgaccgct gaagattttg gcctgactcc ctaccaccag gagcaactgg caggcggaac
    14281 accggaagaa aaccgtgaca ttttaacacg cttgttacaa ggtaaaggcg acgccgccca
    14341 tgaagcagcc gtcgctgcga acgtcgccat gttaatgcgc ctgcatggcc atgaagatct
    14401 gcaagccaat gcgcaaaccg ttcttgaggt actgcgcagt ggttccgctt acgacagagt
    14461 taccgcgctg gcggcacgag ggtaaatgat gcaaaccgtt ttagcgaaaa tcgtcgcaga
    14521 caaggcgatt tgggtagaaa cccgcaaaca gcagcaaccg ctggccagtt ttcagaatga
    14581 ggttcagccg agcacgcgac atttttatga tgcgctacag ggtgcacgca cggcgtttat
    14641 tctggagtgc aagaaagcgt cgccgtcaaa aggcgtgatc cgtgatgatt tcgatccggc
    14701 acgcattgcc tccatttata aacattacgc ttcggcaatt tcggtgctga ctgatgagaa
    14761 atattttcag gggagctttg atttcctccc catcgtcagc caaatcgccc cgcagccgat
    14821 tttatgtaaa gacttcatta tcgaccctta ccagatctat ctggcgcgct attaccaggc
    14881 cgatgcctgc ttattaatgc tttcagtact ggatgacgac caatatcgcc agcttgcagc
    14941 cgtcgctcac agtctggaga tgggggtgct gaccgaagtc agtaatgaag aggaactgga
    15001 gcgcgccatt gcattggggg caaaggtcgt tggcatcaac aaccgcgatc tgcgtgattt
    15061 gtcgattgat ctcaaccgta cccgcgagct tgcgccgaaa ctggggcaca acgtgacggt
    15121 aatcagcgaa tccggcatca atacttacgc tcaggtgcgc gagttaagcc acttcgctaa
    15181 cggttttctg attggttcgg cgttgatggc ccatgacgat ttgcacgccg ccgtgcgccg
    15241 ggtgttgctg ggtgagaata aagtatgtgg tctgacgcgt gggcaagatg ctaaagcagc
    15301 ttatgacgcg ggcgcgattt acggtgggtt gattttggtt gcgacatcac cgcgttgcgt
    15361 caacgttgaa caggcgcagg aagtgatggc tgcggcaccg ttgcagtatg ttggcgtgtt
    15421 ccgcaatcac gatattgccg atgtggtgga caaagctaag gtgttatcgc tggcggcagt
    15481 gcaactgcat ggtaatgaag atcagctgta tatcgatacg ctgcgtgaag ctctgccagc
    15541 acacgttgcc atctggaaag cattaagcgt cggtgaaacc ctgcccgccc gcgagcttca
    15601 gcacgttgat aaatatgttt tagacaacgg ccagggtgga agcgggcaac gtttcgactg
    15661 gtcactatta aatggtcaat cgcttggcaa cgttctgctg gcggggggct taggcgcaga
    15721 taactgcgtg gaagcggcac aaaccggctg cgccggactt gattttaatt ctgctgtaga
    15781 gtcgcaaccg ggcatcaaag acgcacgtct tttggcctcg gttttccaga cgctgcgcgc
    15841 atattaagga aaggaacaat gacaacatta cttaacccct attttggtga gtttggcggc
    15901 atgtacgtgc cacaaatcct gatgcctgct ctgcgccagc tggaagaagc ttttgtcagc
    15961 gcgcaaaaag atcctgaatt tcaggcccag ttcaacgacc tactgaaaaa ctatgccggg
    16021 cgtccaaccg cactgaccaa atgccagaac attacggctg gcacgaacac cacgctgtat
    16081 ctgaagcgcg aagatttgct gcacggcggc gcgcataaaa ctaaccaggt gctcggtcag
    16141 gctttactgg cgaagcggat gggtaaaacc gaaatcatcg ccgaaaccgg tgccggtcag
    16201 catggcgtgg cgtcggccct tgccagcgcc ctgctcggcc tgaaatgccg tatttatatg
    16261 ggtgccaaag acgttgaacg ccagtcgcct aacgtttttc gtatgcgctt aatgggtgcg
    16321 gaagtgatcc cggtgcatag cggttccgcg acgctgaaag atgcctgtaa cgaggcgctg
    16381 cgcgactggt ccggtagtta cgaaaccgcg cactatatgc tgggcaccgc agctggcccg
    16441 catccttatc cgaccattgt gcgtgagttt cagcggatga ttggcgaaga aaccaaagcg
    16501 cagattctgg aaagagaagg tcgcctgccg gatgccgtta tcgcctgtgt tggcggcggt
    16561 tcgaatgcca tcggcatgtt tgctgatttc atcaatgaaa ccaacgtcgg cctgattggt
    16621 gtggagccag gtggtcacgg tatcgaaact ggcgagcacg gcgcaccgct aaaacatggt
    16681 cgcgtgggta tctatttcgg tatgaaagcg ccgatgatgc aaaccgaaga cgggcagatt
    16741 gaagaatctt actccatctc cgccggattg gatttcccgt ctgtcggccc acaacacgcg
    16801 tatcttaaca gcactggacg cgctgattac gtgtctatta ccgatgatga agcccttgaa
    16861 gccttcaaga cgctgtgcct acacgaaggg atcatcccgg cgctggaatc ctcccacgcc
    16921 ctggcccatg cgctgaaaat gatgcgcgaa aacccggaaa aagagcagct actggtggtt
    16981 aacctttccg gtcgcggcga taaagacatc ttcaccgttc acgatatttt gaaagcacga
    17041 ggggaaatct gatggaacgc tacgaatctc tgtttgccca gttgaaggag cgcaaagaag
    17101 gcgcattcgt tcctttcgtc acgctcgggg atccgggcat tgagcagtca ttgaaaatta
    17161 tcgatacgct aattgaagcc ggtgctgacg cgctggagtt aggcatcccc ttctccgacc
    17221 cactggcaga tggcccgacg attcaaaacg ccactctgcg cgcctttgcg gcaggtgtga
    17281 ctccggcaca atgttttgaa atgctggcac tgattcgcca gaaacacccg accattccca
    17341 ttggcctgtt gatgtatgcc aatctggtgt ttaacaaagg cattgatgag ttttatgccc
    17401 agtgcgagaa agtcggcgtc gattcggtgc tggttgccga tgtgccaatt gaagagtccg
    17461 cgcccttccg ccaggccgcg ttgcgccata atgtcgcacc tatcttcatc tgcccgccaa
    17521 atgccgatga cgacctgctg cgccagatag cctcttacgg tcgtggttac acctatttgc
    17581 tgtcacgagc gggcgtgacc ggcgcagaaa accgcgccgc gttacccctc aatcatctgg
    17641 ttgcgaagct gaaagagtac aacgctgcac ctccattgca ggggtttggt atttccaccc
    17701 cggatcaggt aaaagcagcg attgatgcag gagctgcggg cgcgatttct ggttcagcca
    17761 ttgttaaaat catcgagcaa catattaatg agccagagaa aatgctggcg gcactgaaag
    17821 tttttgtaca accgatgaaa gtggcgacgc gcagttaatc cccctgccgc cagttccgct
    17881 ggcggcattt taactttctt taacgaagcc ggaaaaatcc taaattcatt taatatttat
    17941 ctttttaccg tttcgct
//
LOCUS       DAEEKQ010000076        17612 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.076, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000076 DAEEKQ010000000
VERSION     DAEEKQ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17612
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.076"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            12..514
                     /locus_tag="KLK57_002439"
                     /pseudo
     CDS             12..514
                     /locus_tag="KLK57_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
     gene            complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="KLK57_002440"
     CDS             complement(581..2038)
                     /gene="nuoN"
                     /locus_tag="KLK57_002440"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HBE0892788.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPMGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLYSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSASGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="KLK57_002441"
     CDS             complement(2045..3574)
                     /gene="nuoM"
                     /locus_tag="KLK57_002441"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HBE0892789.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRLGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     AEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     EIASQELPGMSLRELFMILLLVVLLVLQGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="KLK57_002442"
     CDS             complement(3738..5579)
                     /gene="nuoL"
                     /locus_tag="KLK57_002442"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HBE0892790.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEILHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAIVGILLA
                     AWLWLGKRTLVSSIANSAPGRLLSTWWYNAWGFDWLYDKVFVRPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="KLK57_002443"
     CDS             complement(5576..5878)
                     /gene="nuoK"
                     /locus_tag="KLK57_002443"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HBE0892791.1"
                     /translation="MIPLQHGLILAAILFILGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="KLK57_002444"
     CDS             complement(5875..6429)
                     /gene="nuoJ"
                     /locus_tag="KLK57_002444"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HBE0892792.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="KLK57_002445"
     CDS             complement(6441..6983)
                     /gene="nuoI"
                     /locus_tag="KLK57_002445"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HBE0892793.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPIYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="KLK57_002446"
     CDS             complement(6998..7975)
                     /gene="nuoH"
                     /locus_tag="KLK57_002446"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HBE0892794.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="KLK57_002447"
     CDS             complement(7972..10698)
                     /gene="nuoG"
                     /locus_tag="KLK57_002447"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HBE0892795.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="KLK57_002448"
     CDS             complement(10751..12088)
                     /gene="nuoF"
                     /locus_tag="KLK57_002448"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HBE0892796.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="KLK57_002449"
     CDS             complement(12085..12585)
                     /gene="nuoE"
                     /locus_tag="KLK57_002449"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HBE0892797.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="KLK57_002450"
     CDS             complement(12588..14390)
                     /gene="nuoC"
                     /locus_tag="KLK57_002450"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HBE0892798.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="KLK57_002451"
     CDS             complement(14484..15146)
                     /gene="nuoB"
                     /locus_tag="KLK57_002451"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HBE0892799.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="KLK57_002452"
     CDS             complement(15162..15605)
                     /gene="nuoA"
                     /locus_tag="KLK57_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HBE0892800.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="KLK57_002453"
     CDS             complement(16234..17172)
                     /gene="lrhA"
                     /locus_tag="KLK57_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HBE0892801.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPIEMMSPYLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4375 a         4929 c         4578 g         3730 t
ORIGIN      
        1 aggaaaaata aatgggaatg attggttatt ttgcagaaat tgactcagaa aagattaacc
       61 aactgcttga gtctactgaa aaacctttga tggataatat acatgacacg ctttcaggat
      121 tacgccgact ggatattgat aaacgctggg attttttaca ctttgggctg acaggcaccc
      181 ctgcttttga tcccgcgaag aatgatccgc tgagtcgtgc cgtgcttggt gaacacagcc
      241 tcgaagatgg catagatggc ttcctgggat tgacctggaa tcaggagtta gccgcgacaa
      301 tcgaccgact ggaatcactg gatcgaagtg agctgcgtaa acagttttcc attaagcgac
      361 tcaatgaaat ggagatttat ccgggagtga ccttttcaga agagttagag gggcaactct
      421 ttgcttcgat catgctgata tggaaaagct gattagcgcg tatcgacgta tgctgcgaca
      481 agggaatcat gcgctgacgg ttattgttgg gtgatggtgt cagggcacca aaaaaaaccg
      541 ccgaacatgt cggcggcttt ctgacttaca aactgatagc ttacatcagc ggcattgcta
      601 accgcacaat gctaatcagc ggttgtggcc atacacccag caccagtacc aacagtgcag
      661 agatcagcac cacgataccg cctgcgctgt actgccagtt tgacggtgca tcgcgacccg
      721 gttgttccgg ggcgtgcagg tacaggctca ccgccacgcg caggtagtag taaaggccgc
      781 ttgccgaacc gacaaccacg gcgcccacca gccaccacaa gtgtgcctgg acaccgaccg
      841 ccagcacgta gaacttacca ataaagccca gcgtcatcgg aataccggcc agagacagca
      901 tcatcaccgt catcaccgcc gcgaggatcg ggcgatgcca gaacagtccg cggtaagagt
      961 acagggaatc agcatccggg ccacgatacg ggctggacat caggctgacc acgccgaacg
     1021 cgccgaggct gctaaacaga taaccggcca ggtaaacccc taccgcttcc atcgacatct
     1081 cgccagtttg cagcgcaatc agcgccacca gcagatagcc gaggtgagag atagatgagt
     1141 aaccgagcag acgtttgata ttggtctggc tcagcgccat caggttaccg aagatgatgg
     1201 aggcaaaggc gataatcgcc agcaccacgc gaatcgcttc gctgtcaccc atcggtgcgt
     1261 agaggaacag acgcatcacc acaccgaaga tagcgatttt gctcgccgtc gccaagaaag
     1321 tggaaaccgg cgcaggcgcg ccctggtata cgtctggcgt ccacaggtgg aacggcacca
     1381 gagagagttt gaagccgagg ccaacaatca tcaggccgaa acctgccagc aacagcggct
     1441 cgttgagcat accgtcgcca aggtttttac ccaacgcgac aaacgacagg tcgccagact
     1501 gcgcatacac cagcgccata ccaaacagca ggaaagaaga cgctgcggca gaaaggatgg
     1561 tgtatttgat actggcttcc agtgaacgtt tctggcggaa agcgtaaccg accaggccaa
     1621 acagcggcaa agagatcagt tcgataccga ggaacagaga cgccagatgg ttggcattcg
     1681 ccagcaggat cccgcccagc gcggcaatta acaccaacag gtagaactcg tccttgttgt
     1741 cgttatagcc ttcaagccac gggtaggcga aagtacaggt ggcgaggctc gccaacaata
     1801 ccagcccggt gtaaagcatg gcgaaaccat caacgcgcat cagcggcgta acgtccatag
     1861 cgcctgcctg gccgacaaac cagagcgaaa ccagcgccgc gttaagccca ataaccgaga
     1921 gcgtagcgtt gaggaaatga ttgcgtcgcc acgcaatgga gagcatcaca accaccaccg
     1981 tcaagccgac gatcagcaac ggtagcagtg cgatcaggtt ttgtggagtt attgtcatgg
     2041 cgatttacgg ccttgtagta gtaacggaat taacaaacca ctgctggata ttgccaatcg
     2101 cggagtgcga ggtatccaga atcggctgcg gatagaagcc ctgcagtacc agcagcacca
     2161 ccagcaacag gatcataaac agctcacgca gcgacatccc tggcagttcc tggctggcaa
     2221 tctcgctttt cgctttaccg aagtaagcgc gatgcaacat cgccagcgaa taaacagatg
     2281 caaagaccag cccaaaggta gagataacgg taatcaccgg gacaacctgg aagctgccga
     2341 acagaatcat aaattcgccg acgaagttac cggtgccagg catcccaagc gttgccaccg
     2401 caaagaacag cgacagtgct ggcagccatt tcatcttgct ccacagaccg cccatcatgc
     2461 gcatgtcgcg ggtatggata cgttcataaa gctgaccaca aagaataaac agacccgccg
     2521 ccgacaagcc gtgcgcaatc atctggatta ccgcgccctg gtaggccaac tggctgccgg
     2581 tgtagatagc aatcagcacg aagcccatgt gggaaaccga ggtgtaggcg atcagacgtt
     2641 tgatatcggt ctgggcgaag gccatccacg caccgtagaa gatgccgata acacccagcc
     2701 acatagcaat tggcgcgaac tctgccgacg cgttcgggaa cagcggcagg gagaaacgca
     2761 gcaaaccgta agcggcagtt ttcagcaaga tccccgcgag gtcaacggaa ccggcggtcg
     2821 gagcctggga gtgcgcatcc ggcagccagc catgcagcgg aaccaccggc attttgactg
     2881 cgaaggcgat gaagaagccc agcattaaca ggtattccgc accactggac attggcgtat
     2941 tcagcagctc ttcatagttg aaggtccaga cgccggtcgc attgtagtga acaaaaacca
     3001 gcgccaggat ggcaatcaac atcaccagac cactcgcctg ggtgtaaatg aagaacttgg
     3061 ttgccgccgt gatacgcgtt ttaccgtcag aggctttatg cccccacagt gcgatcagga
     3121 agtacatcgg caccagcatc atttcccaga agaagaagaa caggaacatg tcgatggcaa
     3181 ggaacacgcc gataacgccg cccaggatcc acatcaggtt gaggtggaag aagccctgat
     3241 atttttcgat ctctttccac gaacatagta ccgccagcac accgagcaga ccggtcagca
     3301 cgaccatcag cagcgacagc ccgtcaatgg cgagatgaat agagataccc aaacgcggga
     3361 tccacggcat gtcgaattca gactgccact gcggaattcc ggcggattgc gtcagtgaat
     3421 aaccgccctg caaccacagt tgcagcgaca gcgccagcgt caatcccatg gtgatcagcg
     3481 cgatccagcg tggcaccttg acgccaaagc gttcggtctg ccagcacagg aagccgccaa
     3541 taaagggaat taatattagc cagggtagta acatggcgat ctttattcct tgtaaaagtc
     3601 ccgtcaggac cggattttca acgaattctc acgacagaaa attctcttcg gactgggggt
     3661 agtgtcggat gcaccgcgaa ggcggcttat ccgacctacg ttcttatatg cccaggggca
     3721 atcccacaat ccttaactca acgcagtacc atcaacagtg ccagcaccac gaccgcaccg
     3781 atgctcatgg atgccacata ccagcgcaga tagccgttct cacttaacag cagaccttta
     3841 cctgcaaagc gggaaaggac agccggaatg ttcatcattg agttcagcgg atcgcgtttc
     3901 agcaaccagg caatacccag gaacggcctg acgaacactt tgtcgtacag ccagtcaaag
     3961 ccccaggcgt tgtaccacca ggtactcagc aggcggcccg gcgcactgtt ggcgatggag
     4021 gacaccagag tacgtttacc cagccacagc caagctgcca gcagaatgcc gacaatcgcg
     4081 accacgccag aggtaatttc cagggtcaac atgctgccgt gcgccagttc cgtcgtttgc
     4141 ggaagcacgc cctgcagcgg cggtacaatc agtgcgccaa cgaaggtgga aaggatcagc
     4201 agcacaatca gcggcaggct gtgagttacc cctttcacgg cgtgagcgtg aatttgttct
     4261 tttccgtgga agacgatgaa aatcatacgg aaggtgtaga gcgaggtcat aaacgcaccg
     4321 accagacctg ccaccatcag attgatatga ccattcgcca tcgcacccgc gaggatctca
     4381 tccttactga agaagcccgc agtgaccagc ggcagtgccg acagtgctgc gccgcccacc
     4441 aggaagcaga gataaaccag cggaatagat ttacgcagac cgcccatctt gaagatgttc
     4501 tgttcgtgat ggcaggccag aatgacggaa ccggatgcca ggaacagcag cgctttaaag
     4561 aacgcgtgag tcatcaagtg gaaaatcgcc gcatcccatg cctgcacgcc aagcgcgagg
     4621 aacatgtagc caatctggct catggtagag taagcgagaa cacgtttgat gtcggtctgt
     4681 accagcgcgg caaaaccggc cagcagcagc gtaaccgccc cgacaatgcc caccagatgc
     4741 agaatttccg gcgtcatcag gaacaggccg tgggtacggg cgatcaggta gacacccgcg
     4801 gttaccatgg ttgcggcgtg gatcagcgcg gagacaggcg tcgggcccgc catcgcgtcg
     4861 gcaagccatg tctgcaacgg caactgcgca gatttaccga ccgcaccgcc cagcagcatc
     4921 agcgtcgccc acatcagcat gttattgccg tcagcaaagt gcgctggtgc cagttccacc
     4981 atttcgcgga agttcagggt gcccagttcg ttgtaaagaa tgaacagtgc gaaagcgagg
     5041 aaaacgtcac ccacacgggt cacgacgaac gctttcattg ccgctgcgcc attcttcgga
     5101 tcggtgtaat agaaaccgat cagcagatag gagcacaggc ccacgccttc ccagccgagg
     5161 tacatcagca gcaggttgtc ggcaagcacc agaactacca tgctggcgat gaacaggttg
     5221 gtgtaggcga agaagcgaga gtagccctct tcaccgcgca tgtaccagga ggcgtacatg
     5281 tgaataagga aacccacacc agtgaccacc gagagcatgg tcagcgacag gccgtccagc
     5341 accaggttaa aaccgatgtt aaagtcgcct accgacatcc acgtccacag cggctggctg
     5401 tatgcctgct cgccgttagc gaagaaatca acgccgataa aggcggttac cagcgccgcc
     5461 aggcccacag agccaacgcc gacgatcgcc gaaacgtttt cagaccagcg cccacgggag
     5521 aacgccagca ggacgaagcc aatcaatggc aaaataatgg ttaaggcaag catgttcatc
     5581 cgcgcatctc acttactgaa tcgatgttca ggttctggcg acgacggtga agttgcagca
     5641 gcagcgcaag gccgatactc gcttctgccg ccgcgaggct gatggcgaga atgtacatca
     5701 cctgaccgtc ggtctgaccc cagtagcttc cggcgacaac aaaggccagc gcggaggcgt
     5761 taatcatgat ttccagacca atcagcataa acagcagatt gcgacggata accagaccgg
     5821 ttaagccaag aatgaataag attgccgcga ggatcagtcc atgttgtaag gggatcatgc
     5881 gtgctcctcc gtttttcttt tcgcgctgtc gtctttacga ttgctcagca cttcacccgc
     5941 acgctcttca cgaccgacgt ggaaggccac aaccagacct gcgagcagca gcatagaagc
     6001 cagttccacc gccagtacgt aaggcccgaa cagcgtaata cccactgctt tagcactgat
     6061 tggcgtaccg tcgataccct gatcgttaac accgaggatg gcgtaaacaa tcaccaccag
     6121 catgatggcc gacaaaattg ccggaccaat ccacacctgc ggtttcagcc actggcgttc
     6181 ctgttcgatt tctgaaccgc ccaggttgag catcatcacc acgaacacga acagcaccat
     6241 aatggcaccc gcgtagacga taatttccag cgcaccggcg aagtaagcgc ccagtgagaa
     6301 gaacaccccg gaaatcgcca gcagcgaaat aatcaggtac agcagtgcgt gtaccggatt
     6361 ggtatgggtg atcactcgca aggtcgcaag tatggctatc aggccacaga tataaaaagc
     6421 gaactccatt gcccctctcc ttacggtaac aggctcttga cgtcgatagg cttggcttcg
     6481 ttctctgctt cgcccttatc tttgccgtcg attgccatac ctgccatccg gtagaagtta
     6541 tattccgggt atttgcccgg accggagatc agcagatcct ctttttcgta aaccagatcc
     6601 tggcgcttgt attcccccat ttcgaaatcc ggggttaact gaatcgccgt ggtcggacag
     6661 gcttcttcgc acagaccaca gaaaatgcag cgtgagaagt tgatgcggaa aaattccgga
     6721 taccagcgac cgtctttggt ttctgctttt tgcagcgaga tacagccgac cgggcaggct
     6781 accgcgcaga ggttacaggc tacgcaacgc tcttcgccgt ccgggtcgcg ggtcagaacg
     6841 atacgaccac gataacgggg cggcagatag atcggctctt ccgggtacat tcgcgtttcg
     6901 cgtttggcga acgcgtgcag gccgatcatc cagatactac gaacctgggt gccgaaacct
     6961 actaacaatt cttttaaggt catggtctta ttgcccctta ttgcgcctgc cagagaatga
     7021 cagccgccgt taccagcaag ttgatcagcg tcagcggcag gcagattttc cagccgaagg
     7081 acattacctg gtcataacgc ggacgcggta acgacgcacg aatcaaaatg aacatcatca
     7141 taaagaacgc ggttttcagc gcgaaccaga tgaatggcgg taacaacggg ccttgccagc
     7201 caccgaagaa cagcgtcacc atcaatgcag agatggtcac aatcccgatg tactcaccca
     7261 cgaagaacag accgaacttc ataccggaat attcaatgtg gtaaccatcc gccagttcct
     7321 gctcggcttc cggctggtca aacgggtgac ggtgacatac cgccacgccc gcgatggcaa
     7381 aggtaataaa accaaagaat tgcgggataa cgttccacac atgcgcctgg ctgttgacga
     7441 tgtcggtcat gttgaatgaa ccggcctgcg ccaccacgcc catcagggaa agcccgagga
     7501 acacttcgta gctcagggtc tgcgcagaag cacgcatcgc acccagcaac gagtatttgt
     7561 tgttacttga ccagcccgca aacagcaccg cgtaaaccgc cagacctgcc atcatcagga
     7621 agaacaaaat cccgatgttc aggtcggcaa ccacccaacc cggactgact ggcacaatcg
     7681 caaaggccag cagcagcgag gtaaaggcaa tcatcggtgc cagggtaaag atgacgcgat
     7741 ccgagaattt cgggatccag tcttctttaa agaacatttt gatcatgtcc gcaaccagct
     7801 ggagcgaacc gccccagcca acacggttag gtccgtaacg gttctggaac agacccagca
     7861 ggcgacgttc gccaaagctc atgaatgccc cgcaggtgac aaccaccagc aggatcacca
     7921 ccgctttgag gatggtcagc aggatctcaa tcagttccgg tgatatccaa ctcattgttg
     7981 tgcctccttg agatcctcaa gatgcgcgcc agccagcacc ggagcaatac cggacatacc
     8041 catcggcaag cccacctgcc ctgccgtcag cccttcagcg atttcaaccg gcagcgtgac
     8101 cgtattgcca tcgtaactaa aggagacgtg tgtacctgcg ttcacaccta acttcgcggc
     8161 atccgctggg ttgagtttga tgtacggctg cggcatacgg ctctggaaga ccggagcacg
     8221 ctgtgacaat tcatcgctgc caaacaggtg gtaatacggc gcgatacgcc atttcccgtc
     8281 ctgcggctgg aagcgtgccg gtacgctggt gaagtaatcc agaccatttt cgctggtttc
     8341 aaacagacgc acgcccggat cgccaaagcg cagtttgccg cccacttcgt cctggaattt
     8401 gttccacgcc tgcggggagt tccagcccgg cgcccaggca aacggcactt gcgaacggtg
     8461 cgcagtcggc tggttgttac cttccatcga gaaggtgaac atggtgtcaa tatcctgcgg
     8521 ctgacgcggc tcatgaacgc tgatattggc gcgcatggcg gtacgaccgc tgtaacggtg
     8581 cggttcacgg gccagtttct gcccacgaat acggaatgtc gcatccggcg cagcatcttt
     8641 gatacctgcc agttccggga ttttcgccac aacagcgtca atcacatggt cgagctgcgt
     8701 ccagtccact tcacggctca gcagggtgct gtgcagcgag tgtaaccagc gccagctttc
     8761 cagcatgaca gttttgctgt cgtaataagc aggatcgtaa acctggaaga aacgttgggc
     8821 gcggccttcg ttgttgatca ccgtaccgtc gctttcagca aaactggcag cagaaagtac
     8881 cagatgggcg ttttccataa tcgctgtgcg ttgatgatca accaccatca ccagcggcgc
     8941 tttagccagc gcagcattca cgcgggtagc ggaagcgtga cgatgcagat cgttttccag
     9001 caccaccacc gcgtcggcgc gtccggtttc cagttcggtc aacgcttctt caagcgaacc
     9061 gccacccata atgcccagcc ccatgctgtt gacggaacgg gcaatcatgg tgataccaac
     9121 gtcagcgccg cgacctttca gggctttcgc gacgttagcc gcagcctgaa tcacctctat
     9181 gctaccggcg ttcgtcccgg agataatcaa cggtttcttc gcacctgcca gtgcctgcac
     9241 gatgacgtcg attttgcttt gcagctcagg ttcgataccg tcaaccgctg gcgcagagtt
     9301 atccagcgca tgggcgatgg caaaacctaa acgcgcctga tcttcaaccg gtgcgcggta
     9361 agtccacgcc gcgatatcat ccagacgggt gtcatcaacg ttggtaacaa acagcggatg
     9421 cttcgcacgt tgaccgatgt tgaggattgc cgcaatctgc cagtcagcca ctttctgtgc
     9481 tgccgccatt tcgcgcgctt tacctttcac agcctgacgc actgccagcg cgacgcgcgc
     9541 gccggtctgg gtaacgtctt cgcccagcac cagtaccgca tcgtaagatt cgatttcgcg
     9601 cagagccgga gtataaatgc cgccttcacg cagcactttc agcgccagtt gcagacgttc
     9661 ctgctcaccg tgagcgatac cggtgtagaa gttttcttcg cccaccagtt cacgcagcgc
     9721 aaagttgctt tccacgctgg cacgcggaga accaataccg atcactttct tcgactgacg
     9781 cagaatatct gccgcgccct gcattgcctg ttcggcgttg agggtaatga aatcatcgcc
     9841 acgacgctgt actggctgac gcggacgatc cttcaggttg acgtaaccgt aaccgaaacg
     9901 accacggtcg cagaggaagt agtggtttac cgtaccgttg taacggtttt cgatacgacg
     9961 cagttcgccg taacgttcac cggggctgat gttacagccg atggaacatt gctggcagat
    10021 gctcggcgca aactgcatat cccatttacg gttgtaacgc tcggagtgcg ttttgtcggt
    10081 aaatacgccg gtcgggcaaa tttcgaccag gttaccagaa aattcgcttt ccagcgtgcc
    10141 gtcttccggg cgaccgaagt agacgttgtc gtgcgcaccg taaacgccca gatctgtacc
    10201 gtcagcgtaa tctttgtagt aacgcacaca gcggtagcag gcgatgcagc ggttcatttc
    10261 gtgagagatg aatggcccca aatcctgatt acggtgggta cgtttggtga aacggtagcg
    10321 acggaagctg tgtccggtca tcacagtcat atcctgaaga tggcagttac cgccttcttc
    10381 acataccgga cagtcgtgcg ggtggttggt cattaaccac tcgaccacac tttcacggaa
    10441 ctgtttcgct tcttcgtcgt caatggaaat aaaggtgcca tcggaagccg gtgtcataca
    10501 ggacatcacc aggcgaccac gcgtgtcttc cgcgttttgg tattgcttca ccgcacactg
    10561 gcggcaagca ccgacgcttc ccagcgccgg atgccagcaa aagtaaggaa tatcaaggcc
    10621 cagagacaga caagcttcca gcaggttgtc cgctccgttg acctcgtatt ctttgccgtc
    10681 tacatgaatt gtagccatta gcatgcttcc agttttctcg gtcagggacc gagcgttaat
    10741 cgaaattcgg ttaccagcgc tctttcagca ggttcggctg aatcccatta atcaaatggg
    10801 tattgctgaa cggctgtttg attcccgcct caaattcttc gcggaaatat ttgatggcgc
    10861 tctgtaacgg ctccactgca ccaggtgcgt gggcacagaa agttttaccc gggcctaaga
    10921 atcgacacag ttgctcaagt gtttcgatat cgcccggctg gccttcgcca cgctccagcg
    10981 cacgcagaat tttcacgctc cacggcagac cgtcgcggca cggcgtacac cagccgcagg
    11041 actcacgggc gaaaaactct tccaggttac gcaccagcga caccatgttg atctcatggt
    11101 caaccgccat cgccagcgcc gtacccagac ggctgcccgc tttaccgata ctttcgaatt
    11161 ccatcggcag atcaaggtgc gcttcggtca ggaagtcagt ccccgcgccg cctggctgcc
    11221 aagctttaaa tttcagacca tcacgcatac caccggcgta atcttcgagg atctcgcgtg
    11281 cggtggtgcc gaacggcagt tcccacagtc ccggattttt cacccgaccg gagaagccca
    11341 tcagcttggt gccagcatct ttacttttcg agatgttctg ataccactcc acgccgttag
    11401 cgaggatcgc cggaacgtta cacagggttt cgacgttgtt gacacaggtc ggtttgcccc
    11461 atacgccgga ggttgccggg aaggggggtt tcgagcgtgg gttagcacga cgcccttcca
    11521 gggagttgat taacgctgtt tcttccccgc agatgtagcg ccctgccccg gtatggacga
    11581 acagttcgaa gtcgaaacct gttcccataa tgtttttgcc aagcaagccc gcttcagtgg
    11641 cttcggcaat ggcgcggcgc agatttactg ccgcttcgat atattcgcca cgcaggaaga
    11701 tgtagccacg gtaagctttc agcgcaaacg cggagatgag cataccttcc accagcaggt
    11761 gcggcagttg ctccatcaac aggcggtctt tataggtgcc cggctccatt tcatcggcgt
    11821 tacacagcag gtaacggatg ttcatggatt cgtctttcgg catcaggctc cacttcaggc
    11881 cagtcgaaaa gcccgctccg ccgcgccctt tcagaccagc gtcttttacc tgattaacga
    11941 tttcgtccgg agacagcccg gtcagcgcct tacgcgcccc ttcgtaaccg tttttgctgc
    12001 ggtattcgtc cagccacact ggctgtttgt catcgcgcag acgccaggtc agcggatgcg
    12061 tttcgggagt acggataatg tttttcattt ataccgctcc agcagttcag ggatcgcttc
    12121 cggggtcaga tgcgcgtgag tgtcctcatc gatcatcatg tttggccctt tatcacagtt
    12181 ccccaggcag caagttggca gcagcgtaaa gcggccatca aatgtcgttt gccctggttt
    12241 gatgttcagc tttttctcga gcgccgcctg aataccctga tacccgttga tatgacagac
    12301 cacgctgtcg caataacgga tcacatggcg accaaccggc tggcggaaga tctgactgta
    12361 gaacgttgcc acaccttcga cgtcgcttgc cggaataccc agcacatcgg cgatcgcgtg
    12421 gatcgcacca tccggcaccc agccacgctg cttctgaacg attttcagcg cttcaatgga
    12481 cgccgcacgc gggtcttcgt agtggtgcat ctcgtgctca atcgcttcac gctctgccgc
    12541 actcagctca aaagcctcgg tttgtggttg ttgattctcg tgcataatta gcggtccaca
    12601 tctgacataa caaaatcgat actgcccaga taaacaatca ggtcagacac caggctgccg
    12661 cggatcgccg ccggaatttg ctgcaaatgc gcataactcg gcgtgcggat acgagtgcga
    12721 taactcatgg tgctaccgtc gctggtcagg tagtaactgt tgatcccctt ggtcgcctca
    12781 atcatctgga aagattcatt ggcaggcatc accggccccc acgacacttg caggaagtgg
    12841 gtgatcaggg tttcgatatg ttgcagcgtg cgctctttcg gcggcggcgt ggtcagcggg
    12901 tgatccgctt tgaacgggcc ttccggcatg ttgttgaggc actgctcaag aatgcgcaga
    12961 ctctggcgca gctcttccac tttcagcatt acgcgggtgt agcagtcaga aacaccacct
    13021 ccaaccggga tttcaaagtc gaagttttca tagccagaat aaggacgcgc cttacgcacg
    13081 tcgaagtcga tcccggtagc acgcaggccc gcgccagtgg tgccccactc cagcgcctct
    13141 ttcgcgccat aggcggcaac gccctgggaa cgacctttca gaatggtatt ttgcagcgcc
    13201 gctttctcgt aagacgccag acgtttcggc atccagtcga ggaactcacg cagcaggcga
    13261 tcccagccgc gcggcaggtc gtgcgctacg ccgccaatac ggaaccacgc cgggtgcata
    13321 cggaaacccg tgatcgcttc caccagatcg taaattttct gacgatcggt aaaggcgaag
    13381 aacacgggcg tcattgcgcc gacgtcctga ataaaggtcg agatgtacag caggtggctg
    13441 ttgatacgga acagttcgga gagcataacg cgaatgacgt taacgcgatc cggcacggtg
    13501 atcccggcca gtttctctac cgccagcacg taaggcattt cgttaacgca gccgccgagg
    13561 tattcaatac ggtcggtata cggaatgtag ctgtgccagg actggcgctc gcccattttc
    13621 tccgcaccac ggtggtggta gccgatgtct ggtacgcagt cgacaatctc ttcgccgtcg
    13681 agttgtaaaa cgatacggaa agccccgtgc gccgacgggt ggttcggacc gaggttgagg
    13741 aacatgaagt cctcgttttc ggtgccgcgc ttcatccccc actcttccgg tttgaaggtc
    13801 agggcttcca tctccagatc ctgtttggct ttggtcagct caaacggcga gaattcggta
    13861 gcgcgcgccg ggtaatcttt acgcagcggg tgacctttcc aggtttgcgg catcatgatg
    13921 cggcgcaggt tcgggtgacc gtcgaaagta atgccaaaca gatcccaggt ttcacgctca
    13981 taccagttag cgttcgggaa cagtttggtg aaggtcggta cgtgcaggtc gttttctgcc
    14041 agcgccacct tcagcatgat gtcgcggtta cgatcgatag aaatcagatg gtagaaaacg
    14101 gaaaaatccg cggcaggtaa cccttcgcgg tgtgtgcgca gacgttcgtc catgccgtgt
    14161 aagtcaaaca gcatgacgta aggtttcggc agtttcttta agaaatcgcc aacttccagt
    14221 aattgttcac gcttgatcca cacaacggga accccggtgc gagtcgcctg aacagtaaag
    14281 gcatccggcc caaaacggtt gcgcagttcg ccaatcaccg gatcatcaag atgatcacgg
    14341 gtctgccagg cgggttcttg cgcggttaag tcggtcatat tgttcaccat tgcaaatggt
    14401 ccgtggtgac tgtcggcgca gtaagcttcg cgaaatagat ttaggaataa gcgaagaaaa
    14461 atccctttgc cgacaggcgc aaattaaatc tcgtcaggtg tacgcaggtt ggttacggca
    14521 atgcgttcac cgcgcttgcg ttcgcgctct gattgcatat tggcgcgata aacgccctga
    14581 tcgccaacca cccaggagag cggacgacgt tctttgccga tagattcctg caacagcatc
    14641 agtgcctgca tgtacgcttc aggacgcggc gggcagcccg ggatatacac atcaaccggg
    14701 atgaatttat cgacgccctg cacaacggaa taaatatcgt acataccacc agagttggca
    14761 caggcaccca ttgagataac ccattttggt tccagcatct ggtcatacag acgctgaata
    14821 accggtgcca ttttggtaaa gcaggttcct gcaaccacca tcaggtcagc ctgacgcggc
    14881 gaagcacgca atacttctgc gccaaaacgc gccacgtcat gcaccgcggt aaacgaagtc
    14941 accatctcaa cgtaacagca ggaaagaccg aagttatacg gccaaattga gtttttacga
    15001 ccccagttaa ccatgtcatt gagcttgccc ataaacacgt ttttgttaac ttcttgctcc
    15061 agagggtcgg ttacgatctc ctgcttttgc agggggtaac ggtcgttctc accgttggga
    15121 tctatgcggg tgagcgtata atccatctta atgcctcgcg gttagcgttg acgattagcg
    15181 atactgttcg tttccgggtt catacgctcg cggcgtgaac gcgcgggcgt ccagtccagc
    15241 gcgccaatac gcaccagata aaccagacct gccagtaaca caaaaataaa aattgcagct
    15301 tccacaaagc ctacccagcc gctttcgcgg atagaggttg accatgcgaa cagatacagc
    15361 gcttcaacgt cgaagataac gaagaacatg gccaccagat aaaacttggc ggacaggcgt
    15421 aagcgggcgg agccgaccga gtcgataccg gattcaaacg gcacgttttt cgacctcgcg
    15481 cgtgcgcgac cgcctaaaaa ccaaccgcct accagcatca ggcaacacag gccaatggca
    15541 acgataagaa agatagcgaa tgcccagtga tgagcgatga cttcagtgga tgttgacata
    15601 ctcattgctt actcatcaaa agtagcgcca gattcactgc tcttcacggc agatggacgc
    15661 cacatcgatt catggggagg aataaaaaaa accttacaat cactgtagaa attcttttat
    15721 acagctaatt gatgtggtct tttactcctt tctataacct tttgtcaact ttaacaaaag
    15781 tttcttcaca ttagtttaca taatatcaac accattagca tttaatgccc tttcacgcca
    15841 gatccttgac gactccagga taattagatg ttgttgaatc gtgtccgttg tgaagcaatg
    15901 gaaaaaatac gggtctattt tgacaggaat ttgtgtcgat tcctcccccc aaaagagagt
    15961 attttcttga tctgtgacac gcttttgtca ttccataaca aaaacgcagc aacaaattta
    16021 cgcattttta acatcattgt agcaggtgat ttttttcagg cgattatttg tgcgttcggg
    16081 acgtgaatct ctggtgattg aaaaatgaac agttttgtac gttctgcact atgcgaggaa
    16141 ggatttttac taaaaaaaag ccgctggggt ttaaaacacc cccagcggct cgtttttaca
    16201 ctattgtctc aggaattatc tatcgtccgt cgattactcg atatcccttt caatcaacaa
    16261 tgaatcatcc ccttccggag cagacattgg gctgtattgc cacggattat ggtagctctc
    16321 catcgcctga tagatcacct gggccagctc attattactg gacgggtcat agcacagcaa
    16381 atattcggta tcaggtagcg gcggtaagcc atctactcca ctcagcacac gcaggtacgg
    16441 gctcatcatt tcaatcggcc ttgccgtcac gccaagaccg gctttcactg ccgcacgaac
    16501 ggccggaagc gtcgaggcga cataagccag tcgccatgga atatctgctt tattaagcgt
    16561 cgccagcacc atatcgcgaa acggacttgg atcatccagc aatacaagag ggatcggctc
    16621 gcctttttgc aatacgtatt ccgctgcgca gtaccagtgt gttggcgagg tacgcaggtt
    16681 caatgcctta aacgctgaag gacgatgggt ggttaccatc aaatccactt cctgggattc
    16741 cagcatttct gccatatagg cattacgttt aacgcggaca tccagcgcta atttcggata
    16801 aaccgaactc acgcgattta acaggaaagg taagatcgta tcggcagatt catctgaagc
    16861 accgatagtt aacacgccct gaagattact gaacattaat gatgagcagg cctcatcatt
    16921 aaaacgcagt attttcctgg cgtaaccaag aagttgaatg ccatgttcag ttaacagttt
    16981 gttgcgaccg tgacgagcga acagttcttt cccaacgagt tgttccagac gctgcatttg
    17041 ctgacttacg gcggactgag tacgacacac agcggcagct gcggcagcaa aagtgttcag
    17101 atcggcaaca gcaacaaatg ttctcagcag atcgaggtcg aggttaatta tcggacgatt
    17161 tgcacttatc atatattatc acttactggc ggctcatact gagctggtta atgctgtgca
    17221 cacacaaaca agcaattcca tttgtaatgt gcctccctgg cagtttcatc cggaacccgg
    17281 acgaaagtaa aaatgcatat gagttgcact aaaaaagcga ctcacattgt tccgttataa
    17341 tgcctgaagt agatcacaga atatatcttc agggatcgca tatctattaa gttactcact
    17401 cttttctatt tatgacatgc gcgtgtttgt ataaatgtaa atgtgagtcc ttgttccact
    17461 ctcgtgcagc atcgctggtc atacgcgaac acgtacaaac agcaatggtg aggcggcatc
    17521 aagagcaggg atccgtcatt tatgcgagca ttttacccca aaaacctttt atttataagg
    17581 gtcattgcga attatctgat gcaaagttat gt
//
LOCUS       DAEEKQ010000077        17448 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.077, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000077 DAEEKQ010000000
VERSION     DAEEKQ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17448
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.077"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..502)
                     /locus_tag="KLK57_002454"
     CDS             complement(<1..502)
                     /locus_tag="KLK57_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001642584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBE0892802.1"
                     /translation="MALTDIKVRTAKPTDKQYKLTDGGGMHLLVHPNGSKYWRLQYRY
                     EGKQKMLALGVYPEITLADARIRRDEPRKLLANGVDPGDKKKSDKVEQSKARTFKEVA
                     IEWHGTNKKWSEDHAHRVLKSLEDNLFAALGERNIAELKTRDLLAPIKAVEMSGRLEM
                     AARLQQR"
     gene            complement(688..772)
                     /locus_tag="KLK57_002455"
     tRNA            complement(688..772)
                     /locus_tag="KLK57_002455"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Leu,seq:caa)
     gene            968..1987
                     /gene="ahr"
                     /locus_tag="KLK57_002456"
     CDS             968..1987
                     /gene="ahr"
                     /locus_tag="KLK57_002456"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HBE0892803.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYEPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKTLAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSVSGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRNGKARYRVV
                     LKADF"
     gene            complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="KLK57_002457"
     CDS             complement(1991..2554)
                     /gene="idnK"
                     /locus_tag="KLK57_002457"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBE0892804.1"
                     /translation="MAGESFILMGVSGSGKTLIGSKVAALLSAKFIDGDDLHPAKNID
                     KMSQGIPLSDEDRLPWLERLNDASYSLYKKNETGFIVCSSLKKQYRDILRKGSPHVHF
                     LWLDGDYETILARMQRRAGHFMPVALLKSQFEALERPQADEQDIVRIDINHDIANVTE
                     QCRQAVLAIRQDRICAKGGSASDQRCE"
     gene            2771..3802
                     /gene="idnD"
                     /locus_tag="KLK57_002458"
     CDS             2771..3802
                     /gene="idnD"
                     /locus_tag="KLK57_002458"
                     /EC_number="1.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HBE0892805.1"
                     /translation="MQVKTQSCVVADKKTVAVTEQTIDWNNNGTLVQITRGGICGSDL
                     HYYQEGKVGNFMIKAPMVLGHEVIGKVIHSDSSELHEGQTVAINPSKPCGHCKYCIEH
                     NENQCTDMRFFGSAMYFPHVDGGFTRYKMVETSQCVPYPAKADEKVMAFAEPLAVAIH
                     AAHQADELQGKRVFVSGVGPIGCLIVSAVKTLGSAEIVCADVSPRSLSLAKEMGADVL
                     VNPQNDDMDHWKAEKGHFDVSFEVSGHPSSVNTCLEVTRARGVMVQVGMGGAMAEFPM
                     MTLIGKEISLKGSFRFTSEFNTAVSWLANGVINPLPLLSAEYLFTDLEEALRFAGDKT
                     QAAKVQLVF"
     gene            3826..4590
                     /gene="idnO"
                     /locus_tag="KLK57_002459"
     CDS             3826..4590
                     /gene="idnO"
                     /locus_tag="KLK57_002459"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HBE0892806.1"
                     /translation="MNDLFSLAGKNILITGSAQGIGYLLATGLGKYGAQIIINDITAE
                     RAELAVEKLHQEGIQAVAAPFNVTHKHEIDAAVEHIEKDIGPIDVLVNNAGIQRRHPF
                     TEFPEQEWNDVIAVNQTAVFLVSQAVTRHMVERKAGKVINICSMQSELGRDTITPYAA
                     SKGAVKMLTRGMCVELARHNIQVNGIAPGYFKTEMTKALVEDEAFTAWLCKRTPAARW
                     GDPQELIGAAVFLSSKASDFVNGHLLFVDGGMLVAV"
     gene            4655..5974
                     /gene="idnT"
                     /locus_tag="KLK57_002460"
     CDS             4655..5974
                     /gene="idnT"
                     /locus_tag="KLK57_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gnt-II system L-idonate transporter"
                     /protein_id="HBE0892807.1"
                     /translation="MPLIIIAAGVALLLILMIGFKVNGFIALVLVAAVVGFAEGMDAQ
                     AVLHSIQNGIGSTLGGLAMILGFGAMLGKLISDTGAAQRIATTLIATFGKKRVQWALV
                     ITGLVVGLAMFFEVGFVLLLPLVFTIVASSGLPLLYVGVPMVAALSVTHCFLPPHPGP
                     TAIATIFEANLGTTLLYGFIITIPTVIVAGPLFSKLLTRFEKAPPEGLFNPHLFSEEE
                     MPSFWNSIFAAVIPVILMAIAAVCEITLPKTNTVRLFFEFVGNPAVALFIAIVIAIFT
                     LGRRNGRTIEQIMDIIGDSIGAIAMIVFIIAGGGAFKQVLVDSGVGQYISHLMTGTTL
                     SPLLMCWTVAALLRIALGSATVAAITTAGVVLPIINVTHADPALMVLATGAGSVIASH
                     VNDPGFWLFKGYFNLTVGETLRTWTVMETLISIMGLLGVLAINAVLH"
     gene            6041..7039
                     /gene="idnR"
                     /locus_tag="KLK57_002461"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
     CDS             6041..7039
                     /gene="idnR"
                     /locus_tag="KLK57_002461"
                     /gene_synonym="gntH"
                     /gene_synonym="yjgS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator IdnR"
                     /protein_id="HBE0892808.1"
                     /translation="MRNHRISLQDIATLAGVTKMTVSRYIRSPKKVAKETGERIAKIM
                     EEINYIPNRAPGMLLNAQSYTLGILIPSFQNQLFADILAGIESVTSVHNYQTLIANYN
                     YDRDSEEESVINLLSYNIDGIILSEKYHTIRTVKFLRSATIPVVELMDVQGERLDMEV
                     GFDNRQAAFDMVCTMLDKRVRRKILYLGSKDDTRDEQRYQGYCDAMMLHNLSPLRMNP
                     RAISSIHLGMQLMRDALSANPDLDGVFCTNDDIAMGALLLCRERNLAVPEQISIAGFH
                     GLEIGRQMIPSLASVITPRFDIGRMAAQMLLSKIKNNDHNHNTVDLGYQIYHGNTL"
     gene            7117..8619
                     /locus_tag="KLK57_002462"
     CDS             7117..8619
                     /locus_tag="KLK57_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HBE0892809.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAEHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="KLK57_002463"
     CDS             complement(8738..9820)
                     /gene="lptG"
                     /locus_tag="KLK57_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HBE0892810.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVASQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="KLK57_002464"
     CDS             complement(9820..10920)
                     /gene="lptF"
                     /locus_tag="KLK57_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HBE0892811.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVVLDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            11187..12698
                     /gene="pepA"
                     /locus_tag="KLK57_002465"
     CDS             11187..12698
                     /gene="pepA"
                     /locus_tag="KLK57_002465"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HBE0892812.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            12832..13275
                     /gene="holC"
                     /locus_tag="KLK57_002466"
     CDS             12832..13275
                     /gene="holC"
                     /locus_tag="KLK57_002466"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HBE0892813.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            13275..16130
                     /gene="valS"
                     /locus_tag="KLK57_002467"
     CDS             13275..16130
                     /gene="valS"
                     /locus_tag="KLK57_002467"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HBE0892814.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGADVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(16186..17382)
                     /locus_tag="KLK57_002468"
     CDS             complement(16186..17382)
                     /locus_tag="KLK57_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HBE0892815.1"
                     /translation="MAQVINDMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLIGCV
                     LTLSLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMALGMGTV
                     FFISTKMLHANSSSSVIISVVLMTIVCIVFIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGIM
                     PSLPFL"
BASE COUNT         4484 a         4335 c         4453 g         4176 t
ORIGIN      
        1 tgcgctgttg aagacgagca gccatttcaa gacgtccaga catttctacg gccttaatag
       61 gtgctaataa atctcgagtt tttaactcag cgatattacg ttcaccaagc gctgcaaaaa
      121 gattatcttc cagacttttt agcacacgat gggcatgatc ttcagaccac tttttattgg
      181 tgccatgcca ctcaatcgcg acttctttaa aggttcgtgc tttactctgt tcaaccttat
      241 cacttttctt tttgtctccc ggatcgacgc cattcgcaag cagcttacgt ggctcgtcac
      301 gacgtattct ggcatccgcg agtgtgattt caggataaac cccaagtgcc agcatctttt
      361 gctttccctc ataacggtac tgcaaacgcc agtacttaga accatttgga tggacaagca
      421 gatgcatacc gccaccatca gtcaacttat attgcttatc tgttggctta gctgttctga
      481 ctttgatgtc tgttaatgcc atgcgtattc cctccctgtt ggtacaagca ttatcgaacc
      541 ggaaatacca tcatctgtac caacatatgt tggtagaagt acgttgatat cggttgacct
      601 tgagagagtt aatatagcgg gaaaggtaga taaatactga atttcaggca taaaaaagac
      661 ctcagttgag gtctattgac atacttttgg tgccgaaggc cggactcgaa ccggcacgta
      721 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
      781 ggtatgcgga aaacgttgtg gattatacct gtcgcacgcc accatgcaag cgccagcttg
      841 ctcgcacctc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
      901 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
      961 ccaaaaaatg tcgatgataa aaagctatgc cgcaaaagaa gcgggcggcg aactggaagt
     1021 ttatgagtac gagcccggtg aattgaagcc acaagatgtt gaagtgcagg tggattactg
     1081 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
     1141 gctggttgcc gggcatgagg tgattgggcg cgtggtggcg ctcgggagcg ccgcgcagga
     1201 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
     1261 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
     1321 tatgaatcgc ggcggttttg ccgagaagtt gcgtgcagac tggcaatggg tgatcccact
     1381 gccggaaaat atcgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
     1441 taaaccactg ttgatgcacc atatcactgc taccagccgc gttggggtaa ttggtattgg
     1501 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
     1561 tagttcgaat cctgcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
     1621 tagccgcgat ccgcaggcac tgaaaacact tgcggggcag ttcgatctca ttatcaacac
     1681 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacgtatg gcggtaattt
     1741 ccacaccgtc ggtgcggtcc tcacgccgct gtctgttccg gcctttacgt taattgcggg
     1801 cgaccgcagc gtctctggtt cagcaaccgg cacaccttat gagcttcgta agctgatgcg
     1861 ctttgcggcc cgcagcaagg ttgcgccgac caccgaactg ttcccgatgt cgaaaataaa
     1921 cgacgccatc cagcatgtgc gcaacggtaa ggcgcgttac cgcgtggttc tgaaagccga
     1981 tttttgaaaa tcattcgcag cgctgatctg aggcgctacc ccctttcgca catattctgt
     2041 cttgtcgtat cgccagcacg gcctgccgac attgttcggt gacattggca atatcatggt
     2101 tgatatcaat gcgtacaata tcctgttcat ctgcttgtgg acgctccagc gcctcaaact
     2161 gactttttag taacgctacc ggcataaaat gcccagcccg ccgctgcatt cgcgcgagaa
     2221 tagtttcata gtcgccatct aaccagagga aatgaacatg ggggctaccc ttgcgtaaaa
     2281 tatcacgata ctgttttttt aatgatgaac agacaataaa tcctgtttca ttctttttat
     2341 aaagactgta tgaagcatca tttaagcgtt ccagccaggg aagtcgatct tcatcagata
     2401 atggaatacc ctgcgacatt ttatctatat ttttggctgg atgaagatcg tcaccatcaa
     2461 taaatttagc agataataac gcggcaacct tgctaccaat taatgtttta ccactccctg
     2521 aaacgcccat caaaataaag ctttcacccg ccatttaaaa tctcctgaat catcttacct
     2581 gctgcgatac tacgcctggt ggcataataa tttcacgttt ttttatttct gctgataaga
     2641 attacaaggc acatcacgtt atgcgtaaca tagtaatgta acaattttct gacgtgatct
     2701 tcatcacaaa taatgacaat taaaaccgct taaatgcttc cgaggtgtct acctgaccag
     2761 tgaggaattt atgcaagtga aaacacagtc ctgcgttgtt gcggacaaga aaactgttgc
     2821 cgttaccgag cagacgatag attggaataa taatggaaca ttagtacaaa taacccgagg
     2881 tggaatttgc ggttccgatt tacattatta tcaggaagga aaagtaggta atttcatgat
     2941 aaaggcaccg atggtgttag gtcatgaagt tatcggtaaa gttattcata gcgactcatc
     3001 agaattacat gaagggcaaa cggtagccat taatccgtct aaaccgtgcg gtcactgcaa
     3061 atactgcatt gaacataacg agaatcagtg tacagatatg cgtttttttg gcagtgccat
     3121 gtatttccct catgttgatg gtggttttac ccgttataaa atggtcgaaa cgtcgcaatg
     3181 tgtcccttat ccggccaaag ctgacgaaaa ggttatggct tttgccgaac ctttagccgt
     3241 cgcgattcat gccgcacatc aggccgacga gttacagggc aagcgagtat ttgtttccgg
     3301 tgttgggccc attggctgcc tgattgtcag tgctgtgaaa acactggggt ccgcggaaat
     3361 tgtctgtgcc gatgtgagtc cccgttccct ttcgctggcc aaagagatgg gtgcggatgt
     3421 gctcgtaaac ccacaaaacg acgacatgga tcactggaaa gcggaaaaag gccatttcga
     3481 tgtcagcttt gaagtgtccg gtcatccttc atcagtgaat acctgtctgg aggtcactcg
     3541 tgcacgcggc gtaatggtgc aggtagggat gggaggcgcg atggcagaat tcccaatgat
     3601 gacgttgatt ggtaaggaga tttcactcaa aggctctttc cgttttacca gcgaatttaa
     3661 taccgcagtg tcatggctgg cgaatggcgt tatcaatcca ctgcctttac tgagtgctga
     3721 atatctcttc actgacctgg aagaggcgct acgtttcgcc ggtgataaaa cccaggcagc
     3781 aaaagtccag cttgttttct aagagataaa taaaggaata atacaatgaa cgatctattt
     3841 tcactggcag gaaaaaatat cttgattacc ggttcagcac agggcattgg ctatttactg
     3901 gcaaccggcc tgggtaaata tggcgcacaa ataattatta atgatattac tgccgaacgc
     3961 gcagaacttg ctgtagaaaa actccaccag gagggtattc aggccgttgc cgcacctttt
     4021 aatgttactc ataaacatga aattgatgcc gccgttgaac atatcgaaaa ggacatcggc
     4081 cccattgatg tgctggtgaa taacgccggt atccagcgcc gtcatccttt tactgagttc
     4141 cctgaacaag agtggaatga tgtgatcgca gtaaaccaga ccgccgtgtt cctggtatcg
     4201 caagcggtaa ctcgtcacat ggttgaacgc aaggcaggta aagttattaa tatttgctcg
     4261 atgcaaagcg aactgggacg tgacaccatc accccttatg ccgcatcgaa aggggcggta
     4321 aaaatgctca cccgcggcat gtgcgtcgag ctggcgcgcc acaatattca ggtcaacggt
     4381 attgcgccgg gctatttcaa aacagaaatg actaaagcac tggttgagga cgaagccttc
     4441 accgcctggt tgtgcaaacg gacccccgcc gcacgctggg gagatccgca ggaactgatt
     4501 ggtgctgcgg tgttcctttc ttcaaaagcc tctgatttcg taaacggcca cctgttgttt
     4561 gttgatggcg gcatgttagt ggctgtttaa cagccaccac cctacgtgca gcgcgaaagc
     4621 gctgcttttc tggcactaat aagagataaa gactatgcca ttaatcatta ttgcggcagg
     4681 cgtcgcgctg cttcttatcc tgatgatcgg ctttaaagtt aacggcttta ttgccctcgt
     4741 tctggtagct gccgtcgtcg gatttgccga agggatggat gcacaggccg tcctgcactc
     4801 tatacaaaat ggtatcggca gcacgctcgg agggctggca atgatcctcg gtttcggggc
     4861 catgttaggc aagctgattt ctgatacggg cgcggcacaa cgtatcgcca ctacgctgat
     4921 tgctactttt ggtaaaaaac gcgtgcaatg ggcgctagtg atcaccggtc tggttgtggg
     4981 cctcgccatg ttttttgaag tgggttttgt cctgctgttg ccgttggtat ttaccatcgt
     5041 ggcatcatct ggattacccc tgttgtatgt tggcgtaccg atggtagcag cgctctctgt
     5101 aacccactgt tttctgccgc cacatccagg gcctactgcc atcgcgacta tctttgaggc
     5161 taatctcgga acgactttac tgtatggatt tatcattacc attccgacag ttattgtcgc
     5221 aggaccgctg ttttctaaac tgctaactcg ctttgagaaa gcaccaccgg aaggcttatt
     5281 taatcctcat ctgtttagcg aagaggagat gccctccttc tggaacagta tttttgctgc
     5341 agtgatcccg gtcatcctga tggctatcgc cgccgtttgt gaaattacgt taccgaaaac
     5401 taacaccgtg cgcctcttct ttgaatttgt cggtaaccct gccgttgcgc tgtttattgc
     5461 cattgttatt gcgattttca cactgggccg acgtaatgga cgcaccatcg agcaaatcat
     5521 ggatatcatt ggggattcta taggcgctat cgcgatgatt gtgtttatta tcgctggcgg
     5581 cggcgcgttt aagcaggtat tagtagatag tggtgtcggg cagtatattt cacacttaat
     5641 gaccggaact acgctttcgc cgttattgat gtgctggact gtcgcggcgc tgttgcgtat
     5701 cgctctgggc tctgccaccg tcgcggccat taccaccgcg ggtgtggtgt tgccgattat
     5761 caacgttacc catgccgatc ccgctttaat ggtactggca actggtgcgg gcagcgtgat
     5821 cgcgtcacac gtaaacgacc ctggcttctg gctatttaaa gggtatttta atctgacggt
     5881 tggtgaaacg ttgcgtacct ggacagtgat ggaaaccctt atttctatta tgggtttgct
     5941 gggcgtgtta gccattaacg ccgtattgca ctgaaagaat aaaatattgt gatgtagttt
     6001 cgggggaaga ttgaagattc ccccgacgtt gggagaagtt atgcgcaatc acagaatttc
     6061 tttacaggat atcgctacgc tggctggcgt aacaaaaatg accgtgagtc gttatatccg
     6121 ctcgccgaaa aaggtggcaa aggaaacagg cgagcgcatc gccaaaatta tggaagagat
     6181 caattatatc cctaatcgcg ctcccggcat gttgttgaat gcacaaagtt ataccctcgg
     6241 tatcttgatt ccgtcatttc aaaatcaact cttcgctgac attctcgcgg gaatagaatc
     6301 cgtcacctct gtgcataatt atcagaccct tattgctaat tacaattacg atcgcgattc
     6361 cgaagaagag tcggtgatta acctgctctc ttataacatt gacgggatta ttctttcgga
     6421 aaaatatcac accatcagga cggtgaaatt tctgcgttca gccaccattc cggtcgtgga
     6481 attgatggat gtacagggag aacggctgga tatggaggtc ggttttgata atcggcaggc
     6541 tgcttttgac atggtgtgta ccatgctgga taagcgagtc aggcgcaaaa ttctgtatct
     6601 cggttccaaa gatgacaccc gcgatgaaca gcgttatcag gggtattgcg atgcgatgat
     6661 gctgcataat ctttccccat tacgcatgaa tccacgcgcc atctcatcca ttcatttagg
     6721 aatgcagtta atgcgtgatg cccttagcgc aaacccggat ttagacggtg tattttgcac
     6781 caatgacgat attgcgatgg gtgcgcttct gctgtgccgc gagcgaaacc tcgccgtgcc
     6841 agaacaaatt tccattgcgg gttttcatgg gctggagatt ggcaggcaaa tgatcccaag
     6901 cctcgccagc gtaattactc cccgttttga tattggtcgg atggccgccc aaatgttgct
     6961 gagcaagatt aagaataacg atcacaacca caacactgtt gacctcgggt atcagattta
     7021 tcacggtaac accctttagg ttattttctc ttcattacac atattctctt tgccggacgt
     7081 tgcctatact ccaggcagga aactggagga aatctcatga gcgaacccct gttaattgcc
     7141 cgcacgccgg acacagaact gtttttactg ccgggaatgg ctaaccgtca cgggctgatt
     7201 actggcgcaa cggggacggg taaaactgtc acgttgcaaa aactggcaga gtcattgtcg
     7261 gaaatcggtg ttccggtgtt tatggccgat gtgaaaggcg atctgaccgg catcgcgcag
     7321 gcaggaacgg cgtcggaaaa actgctcgca agactgaaaa atatcggcgt caacgactgg
     7381 caaccgcatg ccaatccggt ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg
     7441 cgggcgacgg tttcggatct ggggccgctg ttgctggcac gactgttgaa tctcaacgat
     7501 gtgcaatctg gcgtgctgaa tattatcttc cgcattgcgg acgatcaggg attgttgctg
     7561 ctcgacttta aagatttgcg ggcgattacc cagtacatcg gcgataacgc caaatccttc
     7621 cagaatcagt acggaaatat cagtagcgca tcggttggtg ccatccagcg cggattactg
     7681 tcgctggaac agcaaggcgc agaacacttc tttggtgagc cgatgctgga tatcaaagac
     7741 tggatgcgca ccgatgccaa cggtaaaggc gttatcaata tcctcagcgc cgagaaactt
     7801 tatcagatgc cgaaactgta cgccgccagc ctgctgtgga tgctttcaga gttatatgaa
     7861 caattgcctg aagcgggcga tctggagaaa ccaaaactgg tgtttttctt cgacgaagca
     7921 catctgctgt ttaacgatgc gccacaggta ctgctggata agattgagca ggtgataagg
     7981 cttattcgct caaaaggcgt gggcgtctgg ttcgtttcgc aaaacccgtc agatatcccg
     8041 gataacgtgc tagggcagct cggtaatcgc gttcaacacg ctttgcgtgc ttttacgcct
     8101 aaagatcaga aagcagtgaa ggcagcggcg caaaccatgc gggccaatcc ggcgtttgat
     8161 accgaaaagg caatccagga actggggacc ggcgaggcgt taatctcgtt tctcgatgcg
     8221 aaaggaagtc cttcagtggt ggaacgggcg atggtgatcg caccttgttc gcgaatgggg
     8281 ccggtgacgg aagatgagcg taatggcctg attaatcact ccccggtgta tggcaaatat
     8341 gaagatgagg tggaccgcga gtccgcctat gagatgctgc aaaaaggctt tcaggccagt
     8401 accgagcagc aaaataatcc ccccgtgaaa gggaaagagg tggcggtgga tgacggtatt
     8461 cttggtggat tgaaggatat tttgtttggc actaccggac cacgcggcgg gaagaaagat
     8521 ggtgtggtgc aaacaatggc gaaaagcgcc gctcgccagg tgacgaatca gattgtacgc
     8581 gggatgttgg ggagtttgct gggggggaga aaaaggtaag tcagacgtga tctgtgcccc
     8641 tcaccctaac cctctcccca aaggggcgag gggaccgttc gaactcgtat ttgactttgt
     8701 caacaataat aaagggagct ttcgctccct tgttagatta cgattttctc attaacagcc
     8761 acaggctgat taagaagaag ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc
     8821 cataaaccaa cgtcagcggg ccgaagatct ggtccagtac gtagaagaca aaaccgaagc
     8881 tgataccggt gaccacacgc acgcccatcg gtacgctacg cagtgggcca aagatgaacg
     8941 acagcgccat caacatcatc accgccacgg atagcggctg gaagattttg ctccacatgt
     9001 tgagctgata acgtccggca tcctgaccgc tcgacttcag atacttcaca tagttgtgca
     9061 aaccactgat ggagagtgca tccgggtcca gcgccaccac gcccagtttg tctggcgtga
     9121 ggttggtttt ccaggtgccg ctcaccgtct gcgaaccggt gatctgtttc ggattggtca
     9181 gatcagattc atcaacctgc gacagacgcc agactttatg ttccgggtca aacttcgcgg
     9241 tagcggcata acgtacggat tgcagacgac gattctcgtt aaaggcataa atactgatgc
     9301 cacctaactc ttcgtcacct ttaacccgct caatgtagac gaagttgttg ccatctttcg
     9361 cccataagcc ttgctgagtg gagagcaacg agccgccgta catcgcctgc gcacggtagt
     9421 tacgcgccat ctgctcgccc tgcgacgcga cccattcacc aatcgccatc gtcagcaaga
     9481 ccagcggaat ggcggttttc atcaccgaca gcgccacctg catacgggta aaaccagaag
     9541 cctgcatcac caccagttcg ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa
     9601 gcagagccgc catcgggaag aagatttgca catctttcgg cacgctcagc aaggtataca
     9661 ttcctgcgcc taacgcgtcg taactcccct gcccggcttt tttcagctga tcgacaaact
     9721 tgataatgcc cgacagcgac accagcatga acagcgtcat catgatggtg gtgaaaatag
     9781 ttttaccgat atagcggtca agtacgccaa aaggttgcat cacaccgctc ctttacgcga
     9841 aaaactggcg cgcaggcggc ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa
     9901 agccagataa atcaggttaa cggtccacat ccacagcgtc gggtccagct tacctttacc
     9961 gccgttcgat ttcagggagg tctggatcag gaagaaaagt agatacagca gcatggctgg
    10021 cagcatcgac agtacgcgtc cctgacgcgg gttaaccacg ctcaacggta caaccataag
    10081 tgccatcata aacacggtga ataccaacgt gatacgccag ttcagttctg cgcgggcacg
    10141 atcggtgtca gtgttccaca atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag
    10201 caccaccgcc tggtgaccaa tgatcgcctg ataatcctga aagtccgtaa tgcggaaatc
    10261 gcgtaacagt gcagtgcctt cgaagcgcgt tccctggttg agagtgacga cctgggagcc
    10321 gtcgcgcagc tgggttaaat gtccggaatc ggccaccacc acagaaggac gtgcattacc
    10381 ttttggtcga atttgtgcga ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat
    10441 gaacagcacc gagctgccat tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat
    10501 gccagggttc gctttcgctt ctgctaacac ttcatcctga tgacgcgatg accatggtcc
    10561 cgcccacatc acgttaaccg ccgcgacgat tgccgtgaac accgcaagga tcattgccgc
    10621 tttcaccagc accgctttgc tcagaccgca ggcatgcatt accgtaattt cactttcggt
    10681 atacagtttg cccagcgtca tcagcagccc gaggaacagg cttaatggca ggataagctg
    10741 cgccatttcc ggcacgccca acccgagaag ggagagcacc agattcgccg gaatatcgcc
    10801 gtcaaccgct gcgccgagga tcctcactaa cttttgacag aagaagatca aaagcaagat
    10861 gaagagtatc gccagctggc ttttgagcgt ctcccgcacc agatatctta tgattatcac
    10921 tttaaatacg cccgtaaaaa ctcgtctttt gcaggatttt agcttgtttc atggcttaaa
    10981 cgtcatttat tctcttgagt cgtcgaaatc gtcgctaaga taattatact caacggattc
    11041 acctctcaga ttttgttctg acgtgccaat gccgtaataa cgttaagatt aacacgaagt
    11101 catcgcaaca gcggacatga gttacgaaag cttgcaattc tatctgtagc caccgccgtt
    11161 gtctttaaga ttcaggagcg tagtgcatgg agtttagtgt aaaaagcggt agcccggaga
    11221 aacagcggag tgcctgcatc gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg
    11281 cagaacagct cgataaaatc agcgatgggt acatcagcgc cctgctacgt cggggcgaac
    11341 tggaaggaaa accggggcag acactgttgc tgcaccatgt tccgaatgta ctttccgagc
    11401 gaattctcct tattggttgc ggcaaagaac gtgagctgga tgaacgtcag tacaagcagg
    11461 ttattcagaa aaccattaat acgctgaatg atactggctc aatggaagcg gtctgctttc
    11521 tgactgaact gcacgttaaa ggccgtaaca actactggaa agtgcgtcag gctgtcgaga
    11581 ctgcaaaaga gacgctctac agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc
    11641 gtccgctgcg taaaatggtg ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc
    11701 gcgcgatcca gcacggtctg gcgattgccg ccgggattaa agcagcaaaa gatctcggca
    11761 atatgccgcc gaatatctgt aacgccgctt acctcgcttc acaagcgcgc cagctggctg
    11821 acagctacag caagaatgtt atcacccgcg ttatcggcga acagcagatg aaagagctgg
    11881 ggatgcactc ttatctggcg gtcggtcagg gttcgcaaaa cgaatcgctg atgtcggtga
    11941 ttgagtacaa aggcaacgcg tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt
    12001 taaccttcga ctccggcggt atctccatca agccttcaga aggcatggat gagatgaagt
    12061 acgatatgtg cggtgcggca gcggtttacg gcgtgatgcg gatggtcgca gagctacaac
    12121 tgcctattaa cgttattggc gtgctggcag gctgtgaaaa catgcctggc gggcgtgcct
    12181 atcgtccggg cgatgtgtta accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg
    12241 atgccgaagg ccgcctggta ctgtgcgacg tgttaactta cgttgaacgt tttgagccgg
    12301 aagcggtgat tgatgtggcg acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcata
    12361 ttactggtct gatggcgaac cataatccgc tggcccatga actgattgcc gcgtctgaac
    12421 aatccggtga ccgcgcatgg cgcttaccgc tgggtgacga gtatcaggaa caactggagt
    12481 ccaattttgc cgatatggcg aacattggcg gtcgtcctgg tggggcgatt accgcaggtt
    12541 gcttcctgtc acgctttacc cgtaagtaca actgggcgca cctggatatt gcaggaaccg
    12601 cctggcgttc tggtaaagca aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt
    12661 tcctgttaaa ccgcgctggg tttaacggcg aagagtaagt ttgcgtttta ccctcaccct
    12721 aaccctctcc ctaagggaga gggaaccgat cgagcgcaat agaaattcat tgctcatctt
    12781 tcagggtgag gaatgcagca atatttaaat ccaccacaag aagccccata tatgaaaaac
    12841 gcaacgttct accttctgga caatgacacc accgtcgatg gcttaagcgc cgttgagcaa
    12901 ctggtgtgtg aaattgccgc agaacgttgg cgcagcggta agcgcgtgct catcgcctgt
    12961 gaagatgaaa agcaggctta ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc
    13021 tttgtcccgc ataatttagc gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc
    13081 tggccgcaaa aacgcagcag cagcccgcgc gatatattga ttagtctgcg aacaagcttt
    13141 gcagattttg ccactgcttt cacagaagtg gtagacttcg ttccttatga agattctctg
    13201 aaacaactgg cgcgcgaacg ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg
    13261 gcaacctgga aataatggaa aagacatata acccacaaga tatcgaacag ccgctttacg
    13321 agcactggga aaagcagggc tactttaagc ctaatggcga tgaaagccag gaaagtttct
    13381 gcatcatgat cccgccgccg aacgtcaccg gcagtttgca tatgggtcac gccttccagc
    13441 aaaccatcat ggataccatg atccgctatc agcgcatgca gggcaaaaac accctatggc
    13501 aggtcggtac tgaccacgcc gggatcgcta cccagatggt cgttgagcgc aagattgccg
    13561 cagaagaagg taaaacccgt cacgactacg gccgcgaagc tttcatcgac aaaatctggg
    13621 aatggaaagc ggagtccggc ggcaccatta cccgtcagat gcgccgtctc ggcaactctg
    13681 tcgactggga gcgtgaacgc ttcacgatgg acgaaggcct gtccaatgcg gtgaaagaag
    13741 ttttcgttcg tctgtataaa gaagacctga tttaccgtgg caaacgcctg gtaaactggg
    13801 atccgaaact gcgcaccgct atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt
    13861 cgatgtggca catccgctat ccgctggctg acggcgcgaa aaccgcagac ggtaaagatt
    13921 atctggtggt tgcgactacc cgtccggaaa ccctgttggg cgataccggc gtggccgtta
    13981 acccggaaga tccgcgttac aaagacctga ttggcaaata tgtcattcta ccgctggtta
    14041 accgtcgtat tccgatcgtt ggcgacgaac acgccgacat ggaaaaaggc accggctgcg
    14101 tgaaaatcac tccggcgcac gactttaacg actatgaagt gggtaaacgt cacgcgctgc
    14161 cgatgatcaa catcctgacc tttgacggcg atatccgtga aagcgcccag gtgttcgata
    14221 ccaaaggtaa cgaatctgac gtttattcca gcgaaatccc ggcagagttc cagaaactcg
    14281 agcgttttgc tgcacgtaaa gcagtcgttg ctgcggttga cgcactcggc ctgctggaag
    14341 aaattaaacc gcacgacctg accgttcctt acggcgaccg tggcggcgta gttatcgaac
    14401 caatgctgac cgaccagtgg tacgtgcgtg ccgatgtact ggcgaaaccg gcggttgaag
    14461 cggttgagaa cggcgacatt cagttcgtac cgaagcagta cgaaaacatg tacttctcct
    14521 ggatgcgcga tattcaggac tggtgtatct ctcgtcagct gtggtggggt caccgtatcc
    14581 ctgcatggta cgacgaagca ggtaacgttt atgttggccg caacgaagaa gaagtgcgca
    14641 aggaaaataa cctcggtgcc gacgttgccc tgcgtcagga cgaagacgtt ctcgacacct
    14701 ggttctcttc tgcgctgtgg accttctcta ccctaggctg gccggaaaac actgacgcac
    14761 tgcgccagtt ccacccaacc agcgtgatgg tatccggctt cgacatcatt ttcttctgga
    14821 ttgcccgcat gatcatgatg accatgcact tcatcaaaga tgaaaatggc aaaccgcagg
    14881 tgccgttcca caccgtttac atgaccggcc tgattcgtga cgacgaaggc cagaagatgt
    14941 ccaaatccaa gggtaacgtt attgacccgc tggatatggt tgacggtatt tcgctgccgg
    15001 aactgctgga gaaacgtacc ggcaatatga tgcagccgca gctggcggac aaaatccgta
    15061 agcgcaccga gaagcagttc ccgaacggta ttgagccgca cggcaccgat gccctgcgct
    15121 tcaccctggc ggcgctggcg tctaccggtc gtgacatcaa ctgggatatg aagcgcctgg
    15181 aaggttaccg taacttctgt aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca
    15241 cggaaggtca ggattgcggc ttcaacggtg gtgaaatgac gctgtcgctg gcggaccgct
    15301 ggattctggc agagttcaac cagaccatca aagcgtaccg cgaagcgctg gacagcttcc
    15361 gcttcgatat cgctgcaggc attctgtatg agttcacctg gaaccagttc tgtgactggt
    15421 atctggagct gactaagccg gtaatgaacg gtggcaccga agccgaactg cgcggtactc
    15481 gccatacgct ggtgaccgta ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt
    15541 tcatcaccga aaccatctgg cagcgtgtga aagtactttg cggtatcact gccgacacca
    15601 tcatgctgca gccgttcccg cagtacgatg catctcaggt tgatgaagcc gcactggccg
    15661 acaccgaatg gctgaaacag gcgatcgttg cggtacgtaa catccgtgca gaaatgaata
    15721 tcgcgccggg caaaccgctg gagctgctgc tgcgtggttg cagcgcggat gcagaacgtc
    15781 gcgtaaatga aaaccgtggc ttcctgcaaa ccctggcgcg tctggaaagc atcaccgtgc
    15841 tgcctgccga tgacaaaggt ccggtttccg ttacgaagat catcgacggc gcagagctgc
    15901 tgatcccgat ggctggcctc atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag
    15961 tggcgaagat cgaaggcgaa atcagccgta tcgagaacaa gctggcgaat gaaggctttg
    16021 tcgcccgcgc accggaagcg gtcatcgcga aagagcgtga gaagctggaa ggctatgcgg
    16081 aagcgaaagc gaaactgatt gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca
    16141 gtgatgaaaa cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa
    16201 agaaggcata attccccgtg aaatactggc taagaatcct ttatctggtt gttcttcttt
    16261 attgattaaa ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc
    16321 aatcatccat attttcagca gtggataagc cagaccgccc gtaatcccgg atatcaccac
    16381 caacgcacac atgcgataaa gcataccgtg gtatgttaaa gttgagcgaa aacgaatacg
    16441 cccatcattc agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact
    16501 ggtgctaaca gcaatcccaa aataatagat taactgcgcg attatcattt tacgttgcat
    16561 ttccataaat tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc
    16621 atttaatatt tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagccaa
    16681 aaatgcgaga atggcatatt ttatacaata tgtagtatct aatttcacct tgaaacgatg
    16741 tataccgaaa ctggtattac tccagagaaa actcattacc agactatata aagtgccatt
    16801 aaaaatacca atgaaaacaa tacaaactat tgtcatcaga accacagata taataacgct
    16861 acttgaacta ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaag
    16921 ggccattaaa atcggcaaga aaaaggtcac ccaccagaac cctttcatag agcaattaaa
    16981 actaaatctt acgccgttga gactggtcat caaggcctga taacgcagtc cttttgctgc
    17041 cataaatata agcaaaacca acagtgacaa agtcaaaaca cagccaataa gcggcatatc
    17101 tgctgacact gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa
    17161 aacattccca ccggtaatcc cataagaaaa tcgttgtccg ttaacttcca tattagcata
    17221 gagataacgc ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt
    17281 taacaacaca ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa
    17341 cgaatgggac ggaacatcca tatcattaat aacttgagcc ataaaagttc cctgaattaa
    17401 aaaatccatt gatataattt atatatacag acagtaaaat gttctgcc
//
LOCUS       DAEEKQ010000078        17306 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.078, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000078 DAEEKQ010000000
VERSION     DAEEKQ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17306
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.078"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..354)
                     /locus_tag="KLK57_002469"
     CDS             complement(<1..354)
                     /locus_tag="KLK57_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892816.1"
                     /translation="MKVFHNIFKHFSSNHQDKHSDKVNSHQHHGKVDKKYRSNTVEVN
                     MLNDFSQIDNDLGLNIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVI"
     gene            754..1737
                     /locus_tag="KLK57_002470"
                     /pseudo
     CDS             754..1737
                     /locus_tag="KLK57_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(2053..3255)
                     /locus_tag="KLK57_002471"
     CDS             complement(2053..3255)
                     /locus_tag="KLK57_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HBE0892817.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAEDETGFTLDLNGMTVGCEKLVMATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPNVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            3487..4986
                     /gene="pitA"
                     /locus_tag="KLK57_002472"
     CDS             3487..4986
                     /gene="pitA"
                     /locus_tag="KLK57_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HBE0892818.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            complement(5230..5565)
                     /gene="uspB"
                     /locus_tag="KLK57_002473"
     CDS             complement(5230..5565)
                     /gene="uspB"
                     /locus_tag="KLK57_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HBE0892819.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            5956..6390
                     /gene="uspA"
                     /locus_tag="KLK57_002474"
     CDS             5956..6390
                     /gene="uspA"
                     /locus_tag="KLK57_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HBE0892820.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            6708..8177
                     /gene="dtpB"
                     /locus_tag="KLK57_002475"
     CDS             6708..8177
                     /gene="dtpB"
                     /locus_tag="KLK57_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HBE0892821.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLAPVIADRYGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFKPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(8226..8978)
                     /gene="rsmJ"
                     /locus_tag="KLK57_002476"
     CDS             complement(8226..8978)
                     /gene="rsmJ"
                     /locus_tag="KLK57_002476"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HBE0892822.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(8986..11028)
                     /gene="prlC"
                     /locus_tag="KLK57_002477"
     CDS             complement(8986..11028)
                     /gene="prlC"
                     /locus_tag="KLK57_002477"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HBE0892823.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTQALNDCRENVARVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKSVNGLFEVVKRIYGITAKERKEVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            11231..12073
                     /gene="rlmJ"
                     /locus_tag="KLK57_002478"
     CDS             11231..12073
                     /gene="rlmJ"
                     /locus_tag="KLK57_002478"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HBE0892824.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            12145..13497
                     /gene="gorA"
                     /locus_tag="KLK57_002479"
     CDS             12145..13497
                     /gene="gorA"
                     /locus_tag="KLK57_002479"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HBE0892825.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(13551..13634)
                     /locus_tag="KLK57_002480"
     CDS             complement(13551..13634)
                     /locus_tag="KLK57_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HBE0892826.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            13662..13835
                     /locus_tag="KLK57_002481"
     CDS             13662..13835
                     /locus_tag="KLK57_002481"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892827.1"
                     /translation="MHPLTHPLPVTAHVSLLDDDYLTPARASVNGTTRTSDQDFESVY
                     AHYQSENASELTG"
     gene            14374..14727
                     /gene="arsR"
                     /locus_tag="KLK57_002482"
     CDS             14374..14727
                     /gene="arsR"
                     /locus_tag="KLK57_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HBE0892828.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            14781..16070
                     /gene="arsB"
                     /locus_tag="KLK57_002483"
     CDS             14781..16070
                     /gene="arsB"
                     /locus_tag="KLK57_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HBE0892829.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            16083..16508
                     /gene="arsC"
                     /locus_tag="KLK57_002484"
     CDS             16083..16508
                     /gene="arsC"
                     /locus_tag="KLK57_002484"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HBE0892830.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(16642..16938)
                     /locus_tag="KLK57_002485"
     CDS             complement(16642..16938)
                     /locus_tag="KLK57_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0892831.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            17045..>17306
                     /locus_tag="KLK57_002486"
     CDS             17045..>17306
                     /locus_tag="KLK57_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HBE0892832.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAG"
BASE COUNT         4037 a         4444 c         4344 g         4481 t
ORIGIN      
        1 tatcacatgg ttattgtctg gtataattgt aaagataaac gtgttagtag tattatcggc
       61 gaaactatta tattcttgaa tatctataga tggctcacta ttgtaaaacc atattttctc
      121 catctgaaga ctatgttcat ttaccctcca gtgaccatgt tgcaggaaat agatgatatt
      181 caggcctaaa tcgttatcta tttgcgaaaa gtcattcagc atattaactt caactgtgtt
      241 tgatcgatat tttttatcaa ccttgccatg gtgttgatga ctgttaactt tatctgaatg
      301 cttatcttga tggtttgaag agaagtgctt aaatatattg tgaaatactt tcataaataa
      361 cctgtaatat ttttacttgt tagtaacaaa ataaacaata catgtctgaa agacatattt
      421 acgatgatac aaagcaataa tacttatatg ttcgatgatt ttaagcaccg agactatccg
      481 gtcttttact aaataatgga aaacaatcca tccctggaag ataattgtaa aaaaaacaac
      541 agaataagaa ttaatcttag gataaatatt tatttatcat ggccttttaa ccggtaaaaa
      601 atggcactcg gcacagtaaa ccccgctata gttatgctat gcatttgtct ttaatgatat
      661 ctcgtcgtta tattaacagg ctgaaattat cattaatgca catttcatta ttagcaggga
      721 tacctgagga gtttaaagtg aacatatata tcgggtggct ttgtgctgga aagttcaggg
      781 caaagcgagt atttcgtcgc gggtcatgtg ctgatttctc tggcggccat atgcctggca
      841 ttattcacta ccgcatttat tatcatttcg cagctcacgc gcggcgttaa tacgttttac
      901 aatacattgt tccccattat tggctatgcg gggtcaatta tcaccatgat atggggttgg
      961 gcactgttag caggcaatga tgtgatggca gacgagtttg tcgccggcca tgttattttc
     1021 ggcgttggta tgattgccgc ctgtgtatcg acggtggcag cgtcatccgg tcactttctg
     1081 ctcattccca aaaatgcagc ggggagcaag agcgatggaa cgccggtaca ggcttattct
     1141 tcattaatcg gtaattgcct cattgccgtt cccgttttac tcaccctgct cggtttcatt
     1201 tggtctatta cgctgttacg tagtgctgac ataactccgc attatgtcgc gggtcacgta
     1261 ttgcttgggt taaccgcaat ctgtgcctgt ctaattggcc ttgttgccac aattgtccat
     1321 caaacacgta atacgttttc aactaaagaa cactggctgt ggtgttattg ggttattttt
     1381 ctcggctcaa tcacggtact gcaggggata tacgtcttag tcagttccga tgcaagcgcc
     1441 cgactggctc ccggcattat tcttatttgc ctcggaatga tctgttacag catattctca
     1501 aaagtctggc tactggcact ggtctggaga cgtacctgtt cgttagccaa cagaataccg
     1561 atgatacccg tcttcacctg cctgttttgc cttttcctgg catcatttct tgcggaaatg
     1621 gcgcagaccg acatgggata ttttattcct tcgcgagttc tggtcggttt gggagcggta
     1681 tgctttacgt tgttctcaat cgtttcaata ttagaagcgg gttctgctaa aaaataattg
     1741 caacgtaccg gataaaacca gcgttgacca tttgcgtaac gctggttttt cttaggcatc
     1801 atgaaataac gcagcattaa tgcatagtgg ttaaggataa aaaaacaaca aagttactgt
     1861 ctttttcagc ctgttcatat tttataaaga tctggccact gggtgagttt tcaatcacct
     1921 ttccttccac cttatattaa gcctggaggg tttcagttca cgggctcatt agaaaataat
     1981 cacaagatag ccactatcaa aattagctca tttttaatgc ggctctaata attttcatct
     2041 ttaggtaaca ggtcaggacg acttcgctgc aatcaaatcc tgcgcacaag cccacgcact
     2101 cgaccacgcc cactggaagt tatagccccc cagccagccg gtaacgtcca tcacttcgcc
     2161 gatgaagtac agcccaggca ctttgcgcgc ttccatcgtc cgtgaagaga gttcgttggt
     2221 gtccacgccg ccgagcgtca cttcggcagt gcgatagcct tcagtgccgt tgggttgtac
     2281 acgccagtcg gtcaatgtgc taatcagtgc ctgttggtca cgcacgttga gctgttttag
     2341 cgaaacattc gggatttgcc cgagttgctg taagcgttca accaaccgct tcggtagatg
     2401 aaccgccagt gtgtttttca ggctctgatt cggatgtgcg ttacgctgct cattcaggaa
     2461 ggtttcgaga tccacatccg gtagcagatt gatactgaca aattcacccg gttgccagta
     2521 gctggaaatc tgcaacaccg ccggtccaga caagccgcga tgggtgaaga gtaagttctc
     2581 acggaaaacg atgccgtttt cagcggtaat cacggaaggc accgccacgc ccgccagcac
     2641 ctgtaactct tcgagcaacg gtttatgcag agtgaatggc accagacccg cacgggtcgg
     2701 cagcacgttg aggccaaatt gttcggcaat cttataacca aacggcgacg cgcccagccc
     2761 cggcattgac agcccaccgg tcgccatgac cagcttttcg caaccgacag tcatgccgtt
     2821 cagatcaagc gtgaagcctg tttcatcctc cgccacactc agcacttcgc tacgcaatct
     2881 gaaggtcaca ttgcccttct cgcactcatc caccagcatg tcgacaatct gctgcgcgga
     2941 gtcatcgcag aagagttgcc ctaacgtttt ctcgtgccat gcgatgccgt gtttattgac
     3001 cagatcaatg aaatcccact gggtaaaacg tgcgagtgca gacttacaaa aatgcggatt
     3061 ctggctcaga taagcgcctg gttcgacata aaggttggta aagttgcagc gcccaccgcc
     3121 agacataagg attttgcgcc ctggtttttt accattatcg atcagcagaa cccggcgtcc
     3181 tgcctgacct gccagcgcag aacagaacat acccgccgca ccagcgccta taataatggc
     3241 atcaaacctt tccacgttgc gctcctctta gaaaaaacgg gcgtgaattg taaagattcc
     3301 tcagtggtcg caccagcatc aatattacta aaaggaagta tttgcctgaa ttatataaga
     3361 taattatttt ttgagtgaaa tccatacagg aggcaaatca aaaaaagtct atatttcact
     3421 ttgcccgcgc cgcgaaagtc actgataatg cgccgcgttc atgtcctcaa aatggcgtaa
     3481 cgtcctatgc tacatttgtt tgctggcctg gatttgcata ccgggctgtt attattgctt
     3541 gcactggctt ttgtgctgtt ctacgaagcc atcaatggtt tccatgacac agccaacgcc
     3601 gtggcaaccg ttatctatac ccgcgcgatg cgttctcagc tcgccgtggt tatggcggcg
     3661 gtgttcaact ttttgggtgt tttgctgggt ggtctgagtg ttgcctatgc cattgtgcat
     3721 atgctgccga cggatctgct gcttaatatg ggatcgtctc atggccttgc catggtgttc
     3781 tctatgttgc tggcggcgat tatctggaac ctgggtacct ggtactttgg tttacctgca
     3841 tccagctctc atacgctgat tggcgcgatc atcgggattg gtttaaccaa tgcgttgatg
     3901 accgggacgt cagtggtgga tgcactcaat atcccgaaag tattaagtat tttcggttct
     3961 ctgatcgttt cccctattgt cggcctggtg tttgctggcg gtctgatttt cttgctgcgt
     4021 cgctactgga gcggcaccaa gaaacgcgcc cgtatccacc tgaccccagc ggagcgtgaa
     4081 aagaaagacg gcaagaaaaa gccgccgttc tggacacgta tcgcgctgat cctttccgct
     4141 atcggcgtgg cgttttcgca cggcgcgaac gatggtcaga aaggcattgg tctggttatg
     4201 ttggtattga ttggcgtcgc gccagcaggc ttcgtggtga acatgaatgc cactggctac
     4261 gaaatcaccc gtacccgtga tgccatcaac aacgtcgaag cttactttga gcagcatcct
     4321 gcgctgctca aacaggctac cggtgctgat cagttagtac cggctccgga agctggcgca
     4381 acgcaacctg cggagttcca ctgccatccg tcgaatacca ttaacgcgct caaccgcctg
     4441 aaaggtatgt tgaccaccga tgtggaaagc tacgacaagc tgtcgcttga tcaacgtagc
     4501 cagatgcgcc gcattatgct gtgcgtttct gacactatcg acaaagtggt gaagatgcct
     4561 ggcgtgagtg ctgacgatca gcgcctgttg aagaaactga agtccgacat gcttagcacc
     4621 atcgagtatg caccggtgtg gatcatcatg gcggtcgcgc tggcgttagg tatcggtacg
     4681 atgattggct ggcgccgtgt ggcaacgact atcggtgaga aaatcggtaa gaaaggcatg
     4741 acctacgctc aggggatgtc tgcccagatg acggcggcag tgtctatcgg cctggcgagt
     4801 tataccggga tgccggtttc cactactcac gtactctcct cttctgtcgc ggggacgatg
     4861 gtggtagatg gcggcggctt acagcgtaaa accgtaacca gtattctgat ggcctgggtg
     4921 tttacccttc cggctgcggt actgctttcc ggcgggctgt actggctctc cttgcagttc
     4981 ctgtaatcgt acgcaccaaa acgagcgggt cagctggccc gcttcagatt gtgacatagt
     5041 gcgctttgtt catgccggat gcggcgtgaa cgccttatcc ggcctacaaa aacatgcaaa
     5101 ttcaatagat tgcagagatt atgtaggcct gataagcgta gcgcatcagg caattttgcg
     5161 tttgtaatca gtctcgagcg ggtcagttta ctggcccgct ttttttatgc gttattattg
     5221 ctgaagtgct taatgccaaa tcatcaatgc aatcaggctg accaccacca gaccacacaa
     5281 tgcgctggtc agaataaact gccgacgcac ccgctcacag cggcgaataa attcatcatc
     5341 gtgatgatcg cgataacgtt gggcatagat ataccaaacg agacgcacct gtttgttggg
     5401 ttggccatgt gaggtaaaaa agccccctcc atcaacatat tgatagagca atggatcgca
     5461 gttacgcagt accactaaca acgcgcgtag tgatgagaaa tagcgcgcca tgttaacaat
     5521 gcaaacgaca cataaagccc aaaataatgc gacggtgctt atcatacctc ctccccggcg
     5581 acctgcccgc ggagttccac cccggggcta ccgctcccga tacgctgcca atcagttaac
     5641 accaggtcct ggagaaaccg cttttgtggt gaccaacatc cgagcggctc tatagatagt
     5701 gtaggagatc aggttgtttt ttttccagaa ggttaaccac tatcaatata ttcatgtcga
     5761 aaatttgttt atctaacggg taagcaaggc ggattgacgg atcatccggg tcgctataag
     5821 gtaaggatgg tcttaacact gaatctttac ggctgggtta gccccgcgca cgtagttcgc
     5881 aggacgcggg tgacgtaacg gcacaagaaa cgctagctgg ccagtcatcg acaactttat
     5941 ggaaggagta acactatggc ttataaacac attctcatcg cggtcgatct ctccccggaa
     6001 agcaaagttc tggtagagaa agcagtctct atggctcgcc cctacaatgc gaaagtttct
     6061 ctgatccacg tagatgtaaa ctactctgac ctatacaccg ggcttattga tgtgaatctg
     6121 ggtgatatgc agaaacgcat ctctgaagag acacatcatg cgctgaccga gctttccact
     6181 aatgcaggct acccaatcac tgaaaccctg agcggcagcg gcgacctggg ccaggttctg
     6241 gtcgatgcaa tcaagaaata cgatatggat ctggtggttt gtggtcacca ccaggacttc
     6301 tggagcaaac taatgtcttc cgcacgtcag ctgatcaaca ccgttcacgt tgatatgctg
     6361 attgttccgc tgcgcgacga agaagaataa tcatcccccc acgacgtgtt cctgaacgcc
     6421 cgcatatgcg ggcgttttgc tttttggcgc gccttgttac ctgatgcagc gtaaacccct
     6481 tatctggcct acgatctgcg tacgcaatca aaatctcctg ccaatacaac atttaacact
     6541 atcatatttt ccatcattag tgtgatcagc tggttatttt ctgttgtaat agtgtattaa
     6601 tctattcacc gcatcaatat taagaatctc tgacagatgt aaactttttc gcgcgttatc
     6661 ccttacgcgt tcatactttt caggatggta ttggaaggtt aataaatatg aatacaacaa
     6721 cacccatggg gatgctgcag caacctcgcc catttttcat gatctttttt gtcgagttat
     6781 gggagcgatt cggctactac ggcgtgcagg gcgtactggc ggttttcttc gttaaacagc
     6841 ttggattctc gcaagagcag gcttttgtca cttttggtgc ttttgctgcg ctggtctatg
     6901 gcctcatttc cattggcggc tatgtcggcg accacctgct ggggaccaaa cgcaccattg
     6961 ttctcggagc acttgtgctg gcgattggct acttcatgac cggcatgtcg ctacttaagc
     7021 ctgacctgat tttcatcgcc ctggggacta tcgctgtcgg taacggcctg tttaaagcta
     7081 acccagccag cttgctttcg aagtgctatc cgccgaaaga tccgcggctt gatggcgcat
     7141 tcaccctgtt ctatatgtcg atcaatatcg gctcgttgat agcgttatcg ctggcccctg
     7201 tgatcgctga tagatacggt tattcagtca cctacaacct gtgcggggcg gggttaatta
     7261 tcgcattact ggtttacatc gcctgtcgtg gaatggtgaa agacattggt tctgaacccg
     7321 acttcaagcc gatgagcttc agcaaactgt tgtacgtatt acttggcagc gtggtgatga
     7381 tcttcgtatg tgcatggctg atgcacaacg tagaagtcgc caatctggtg ctgattgttc
     7441 tctccatcgt cgtcaccatc atcttctttc gtcaggcatt caagctggat aaaaccgggc
     7501 gcaataaaat gtttgtcgcc tttgtcctga tgctcgaagc ggtggtgttt tacattctct
     7561 acgcccagat gccaacgtcg ctgaacttct ttgccatcaa caacgtgcat catgaaattc
     7621 tcggcttttc cataaacccg gtcagcttcc aggcgcttaa cccgttctgg gtggtactcg
     7681 ccagcccaat actggcaggc atttacacgc atctgggtaa caaaggcaaa gacctctcga
     7741 tgccgatgaa atttactctc ggcatgttta tgtgctcact gggctttttg acggcggcag
     7801 ctgcgggaat gtggtttgcg gatgcacaag ggctgacatc gccatggttt atcgtgctgg
     7861 tgtatttatt ccagagcctg ggtgaattgt ttattagcgc ccttggcctg gcgatgattg
     7921 ctgccctggt gccgcagcat ttgatgggct ttattctcgg gatgtggttc ctgacgcagg
     7981 ctgccgcatt cttgctgggc ggctatgtgg caacatttac cgcagtgccg gacaacatta
     8041 ccgatccgct tgagacgtta cccgtctata ccaacgtgtt tggtaagatt ggcctggtta
     8101 cgctgggcgt tgcagtagtg atgctgttga tggtgccgtg gctgaaacgc atgattgcga
     8161 cgccggaaag ccattaatta ttcttgcaga aagcagggta gcgttatcgc taccctgctt
     8221 tacttttaca cgggcgtacc tgcatagata tcaaagcgat gccctttagt cacaaccgcg
     8281 tttggcgtgg cgacattcgc tagcggtggc gcgtagtccg ggcgctttac taccacgcgt
     8341 ttggtcgcca gcaagcgtgc aggctccagc aatccatcgg catcaagatc cggccccacc
     8401 agcgactgaa agacacgcat ctcttttttc accagcgcgc ttttctgctt atgcgggaac
     8461 atcgggtcga gatagaccac ctgcgggcgt ggggtaatat cggttagcgc cgtcaggctg
     8521 gaggcgtgaa ttaactgcaa acgctcctgc aaccagccgc cgatttccgc atccgcataa
     8581 ccacgcgcca ggccgtcgtc gagcagcgcg gcaacaactg gattacgctc cagcatccgc
     8641 acgcggcaac cgactgaagc cagcacaaag gcatcgcgcc ccagtcctgc agtggcatcc
     8701 accacatccg gcaaataatc gcctttaatg cccaccgctt tcgccaccgc ctcaccgcga
     8761 ccaccgccga atttgcgtcg gtgcgccatc gctccgccaa caaaatcaac aaagatgccg
     8821 ccaagttttg gctcatcacg cttgcgcaat tccagatgtt ccggcgttaa caccagcgcc
     8881 atcaggttgt cttcatcgtg ctccagcccc cagcgggccg ccagaacaga taaggcgccg
     8941 tctccggcgc ctgtttcatc aattaagcag attttcactg aatgatcagc ccttaatgcc
     9001 gtaatgctcc agcatcgcat ccagctgcgg ttcacgacca cggaagcgtt tgaacagatc
     9061 catcggctct tctgaaccgc cacggctcag aatgttgtcg aggaacgact gcccggtttc
     9121 acggttgaaa atgccctctt cctcaaagcg cgagaaagca tctgccgcca gtacgtcagc
     9181 ccacaggtag ctgtagtaac ctgccgcata accaccggcg aaaatatggc tgaaagcgtg
     9241 cgggaaacgg ccccaggacg gagatggcac cacggcaacc agtttcttga tttctgccag
     9301 agtttcgagg atttttgccc cctgatccgg gcggaactcg gcatgaaggc ggaaatcaaa
     9361 caggccgaac tccagctgac gcagaataaa cagcgccgcc tggtagttct tcgccgccag
     9421 cattttatcc agcaactctt tcggcagcgg ttcgccggtt tcatagtgac cagagataaa
     9481 cgccagcgcc tccggctccc agcaccagtt ttccataaac tgactcggca gttcgaccgc
     9541 atcccacggc acaccgctga taccggaaac accagcggtt tcgatgcggg tcagcatatg
     9601 gtgcaggccg tgaccgaact cgtggaacag ggtgatcact tcgtcgtgag tgaacagcgc
     9661 cggtttacca tttaccgggc ggttgaagtt acaagtcaaa tacgcgaccg gtttttgcag
     9721 agaaccatca gctttacgca tctggcctac gcagtcatcc atccacgccc cgccgcgctt
     9781 gttttcacgg gcatacagat cgaggtagaa gctgccgcgc agttcgttat tttcgtcata
     9841 cagttcgaag aaacgtacat ccggatgcca gacatcaacc tctttacgct ctttagcggt
     9901 gatgccgtaa atacgtttca ccacttcaaa caggccgtta accgatttgt tttccgggaa
     9961 gtacggacgc agctgctcgt cgctgatgct gtagaggtgc tgtttctgtt tttcgctgta
    10021 gtaagcgata tcccacggct gcaactcatc gacaccaaat tcggctttag cgaaggcacg
    10081 cagttgctcc agctcttttt cgccttgcgg acgcgcgcgt tttgccagat cggttaagaa
    10141 atccagcacc tgctgcgggt tttctgccat tttagtggca agggatttaa aggcgtagtt
    10201 ttcaaagccc agcagttgcg ccagttcgtg acgcagcgcg aggatctctt ccatcacctt
    10261 gctgttatcc cacttaccgg cgttcgggcc ttgatcggag gcgcgggtgc tgtaagcgcg
    10321 atacatctct tcgcgcagag cctggttgtc gcagtaggtc attaccggca ggtagcttgg
    10381 gatatccagc gtcagcaggt agccttccag ctctttcgct tcggcctggg cttttgccgc
    10441 agccagcgcg ctttctggca tccccgccag ccccgcttcg tcggtaacga gtttggtcca
    10501 gcccatcgtc gcatcgagga cgttgttgct gtactggttg cccagttcag aaagacgggt
    10561 agcaatttcg ccgtaacgct gctgtttctc tttcggcaga cctatgccag agagttcaaa
    10621 atcacgcagg gcgttatcaa ccgctttctt ctgcgccgtg ttcagcgtgg cgtaatgatc
    10681 gccatcgcgc aggtcgcggt acgctttata cagcccttca tgttgcccta cccaggtgct
    10741 gtattccgac agcagcggca gggtttgctc gtaggcttca cgcagttccg ggctattttt
    10801 caccgagttc aggtggctga ccggggagaa gatacgcccc aacacatcgt ccacttccgc
    10861 caacggctgg cagagatttt cccaggtgta cggtgcccct tgcgctacta cgcgcgccac
    10921 attttcgcgg caatcgttca gcgcctgagt cacggctgga acgacatgtt ccgggagaat
    10981 tttagaaaac ggaggcaatt caaagggagt cagtaacgga ttcgtcattc gcgcagtcct
    11041 ggttaaagag gttaaggaag cgctcaacag gcgctttaca taatgtgtgt agcatggggt
    11101 taagtgtagt gaatttcaat gagaaacgtt acgctttcgc ggcggcggcc tcttttcggt
    11161 atactgtcct gatacgcttt tgtgcgcccc gaatacgggc cgatttttac ttaccggaac
    11221 acctttaccc atgctcagtt atcgccacag ctttcacgct ggcaaccacg ccgacgtcct
    11281 taaacatacc gttcagagcc tgatcatcga gtcgctgaaa gagaaagata aaccgtttct
    11341 ctatctcgat acccacgcag gggccgggcg ttatcagtta ggcagcgaac atgccgagcg
    11401 taccggcgaa tatctcgaag gtatcgcccg tatctggcag caggacgatt tgcccgcaga
    11461 actggaggcg tacatcaatg tggtaaaaca cttcaaccgt agcggtcagt tgcgttacta
    11521 ccccgggtcg ccgttgattg ctcgccagct actgcgcgaa caagacagcc tgcaactgac
    11581 cgaactgcac ccgagcgatt acccgttgct gcgttctgaa tttcagaaag acagccgtgc
    11641 gcgcgtcgaa aaagccgacg gtttccagca gcttaaggcc aaacttccgc cggtttcccg
    11701 ccgtggttta atccttatcg acccgccgta tgaaatgaaa accgactatc aggcggtggt
    11761 cagcgggata gcagaaggtt acaaacgttt cgccactggt acttacgcac tgtggtatcc
    11821 ggtggtgctg cgtcagcaaa ttaagcgcat gatccacgat ctggaagcga ccggtattcg
    11881 caaaattctg caaattgaac tggcggtact gccagacagc gatcgccgtg gcatgaccgc
    11941 ttccggcatg attgtgatta acccgccgtg gaaactggaa cagcagatga ataacgtgct
    12001 gccgtggctg cacagcaaac tggttccggc aggcaccggg cacgccaccg taagctggat
    12061 cgtgccggag taattgcagc cattgctggc acctattacg tctcgcgcta caatcgcggt
    12121 aatcaacgat aaggacactc tgtcatgact aaacactatg attacatcgc catcggcggc
    12181 ggcagcggcg gtatcgcctc catcaaccgc gcggctatgt acggccagaa atgcgcactg
    12241 attgaagcca aagagctggg cggcacctgc gtgaacgtcg gctgcgtgcc gaaaaaagtg
    12301 atgtggcacg cggcgcaaat ccgtgaagcg atccatatgt acggccctga ttatggtttc
    12361 gataccacca tcaacaagtt caactgggaa acgttgatcg ccagccgtac cgcctatatc
    12421 gaccgtattc atacttccta tgaaaacgtg ctcggtaaaa ataacgttga tgtaatcaaa
    12481 ggctttgccc gcttcgttga tgccaaaacg ctggaggtaa acggcgaaac catcacggcc
    12541 gatcatattc tgatcgccac aggcggtcgt ccgagccacc cggatattcc gggcgtggaa
    12601 tacggtattg attctgatgg cttcttcgcc cttcctgctt tgccagagcg cgtggcggtt
    12661 gttggcgcgg gttacatcgc cgttgagctg gcgggcgtga ttaacggcct cggcgcgaaa
    12721 acgcatctgt ttgtgcgtaa acatgcgccg ctgcgcagct tcgacccgat gatttccgaa
    12781 acgctggtcg aagtgatgaa cgccgaaggc ccgcagctgc acaccaacgc catcccgaaa
    12841 gcggtagtga aaaatgccga tggtagcctg acgctggagc tggaagatgg tcgcagtgaa
    12901 acggtggatt gcctgatttg ggcgattggt cgcgagcctg ccaatgacaa catcaaccta
    12961 gaagccgctg gcgttaaaac taacgaaaaa ggctatatcg tcgtcgataa atatcaaaac
    13021 accaatattg aaggtattta cgcggtgggc gataacacgg gtgcagtgga actgacaccg
    13081 gtagcagttg cagcgggtcg ccgtctctct gaacgcctgt ttaataacaa gccggatgag
    13141 catctggatt acagcaacat tccgaccgtg gtcttcagcc atccgccgat tggtactgtt
    13201 ggtttaacgg aaccgcaggc gcgcgagcag tacggcgacg atcaggtgaa agtgtataaa
    13261 tcctctttca ccgcgatgta taccgccgtc accactcacc gccagccgtg ccgcatgaag
    13321 ctggtgtgcg ttgggccaga agagaagatt gtcggtattc acggcattgg ctttggtatg
    13381 gacgaaatgt tgcagggctt cgcggtggcg ctgaagatgg gcgcaaccaa aaaagacttc
    13441 gacaataccg tcgccattca cccaacggcg gcagaagagt tcgtgaccat gcgctaaatg
    13501 ttaaagggct aagagtagtg tgctcttagc ccttaattac gtttccgcta tcagttcaga
    13561 agctgaagca gaaagcggat cagttccagc agcgcaatta acgctccaag aacgatgatt
    13621 gctttatcaa tcacccgttt tctccatgcg atggagtgag aatgcatccg cttactcatc
    13681 cactgcctgt cacggcgcat gtctcattgt tagatgacga ctatctcact ccggccagag
    13741 catcagttaa cggcaccacc cgtacttctg accaggactt tgaaagcgtt tatgcgcatt
    13801 accagagtga aaatgcctca gagctaactg gataatcata cagtacattc aggttataaa
    13861 accagcatgt ccttgcaata gtttcagtat ggtattagca ttgatgcgtt agatgatgac
    13921 tatctcactc cagccagagc caccaactca gggctgaaga gtaaaaaaac cgacgcaaag
    13981 tcggtttttt tacatccgga ttcggacaag gcttaatatg acgatgaccc agtgaaagta
    14041 tataaatcgt cattgcgata tataccgaag tgccccctcc gccagctgaa gaaatcgcta
    14101 attcttgcaa tgttagccac tggctaatag tattgaaccg ttagatgtgg gcattctcac
    14161 tccagccaga gccaccaact caggctgaag agtaaaaaac cgacgcaaag tcggtttttt
    14221 tacgtcctga ttcaaaccgc ctttcaaatg aatagccaac tcaaaattca cacctattac
    14281 cttcctctgc acttacacat tcgttaagtc atatatgttt ttgacttatc cgcttcgaag
    14341 agagacacta cctgccacaa tcaggagcgc aatatgtcat ttctgttacc catccaattg
    14401 ttcaaaattc ttgctgatga aacccgtctg ggcatcgttt tactgctcag cgagctggga
    14461 gagttatgcg tctgcgatct ttgtactgcc ctcgatcagt cgcagcccaa gatctcccgc
    14521 cacctggcat tgcttcgtga aagcgggcta ttgctggacc gcaagcaagg taagtgggtt
    14581 cattaccgct tatcaccgca tattccagca tgggcggcga aaattattga tgaggcctgg
    14641 cgatgtgaac aggaaaaggt tcaggcgatt gtccgcaacc tggctcgaca aaactgttcc
    14701 ggggacagta agaacatttg cagttaaaaa tttagctaaa cacatatgaa ttttcagatg
    14761 tgttttatcc gggaggcatt atgttactgg caggcgctat ctttgtcctg accatcgtat
    14821 tggttatctg gcagccgaaa ggtttaggca tcggctggag tgcaacgctc ggcgcagtac
    14881 tggcgttagt tacgggcgtg gttcatccgg gtgatattcc ggtggtgtgg aatatcgtct
    14941 ggaacgcgac ggctgcgttt atcgccgtca ttatcatcag cctgctgctg gatgagtccg
    15001 gcttttttga atgggcggcg ctgcacgtct cacgctgggg taatggtcgt ggtcgcttgc
    15061 tgtttacctg gattgtcctg ctcggtgctg ccgttgccgc cctgtttgcc aatgatggcg
    15121 cggcgcttat tttgacgccg attgtcatcg ccatgctgct ggctttagga ttcagtaaag
    15181 gcacgacgct ggcgttcgtg atggcggccg gattcattgc cgataccgcc agcctgccgc
    15241 ttattgtctc caacctggtg aatatcgttt ccgctgattt ctttggcctc ggctttcgcg
    15301 aatacgcctc ggtgatggtg ccggtggata tcgccgcgat tgttgccacg ctggtgatgc
    15361 tacatctcta ttttcgcaaa gatattccgc agaactacga tatggcgctg ctgaaatctc
    15421 ccgcagaagc gatcaaagat cctgctacgt tcaaaactgg ctgggttgtt ttactgcttc
    15481 tgctggtggg atttttcgtc ctggaaccgc tcggcattcc ggtgagcgcc attgcagctg
    15541 tgggcgcgct gatattattt gtcgtcgcta aacgcggtca tgcgattaat acgggtaaag
    15601 tcctgcgcgg tgccccctgg cagattgtca tcttctcgct cggcatgtat ctggtggttt
    15661 atggcctgcg caatgccgga ttaacggaat atctttctgg cgtactcaac gtgctggcgg
    15721 ataacggcct gtgggccgcg acgctcggca ccggattcct caccgccttc ctctcttcta
    15781 ttatgaacaa tatgccgacg gtactggttg gcgcgttgtc cattgatggc agcacggcat
    15841 ctggcgttat caaagaagcg atggtttatg ccaatgtgat tggctgcgat ttgggaccga
    15901 aaattacccc aattggtagc ctggctacgc tactctggct gcacgtactt tcgcagaaga
    15961 atatgactat cagctgggga tattacttcc gtacagggat tatcatgacc ctgcctgtgc
    16021 tgtttgtgac gctggctgcg ctggcgctac gtctctcttt cactttgtaa tgagatactg
    16081 atatgagcaa cattaccatt tatcacaacc cggcctgcgg cacgtcgcgt aatacgctgg
    16141 agatgatccg caacagcggc acagaaccga ccattatcca ttatctggaa actccgccaa
    16201 cgcgcgatga actggtcaaa ctcattgccg atatggggat ttccgtacgc gcgctgctgc
    16261 gtaaaaacgt cgaaccgtat gaggagctgg gccttgcgga agataaattt actgacgatc
    16321 ggttaatcga ctttatgctt cagcacccga ttctgattaa tcgcccgatt gtggtgacgc
    16381 cgctgggaac tcgcctgtgc cgcccttcag aagtggtgct ggaaattctg ccagatgcgc
    16441 aaaaaggcgc attctccaag gaagatggcg agaaagtggt tgatgaagcg ggtaagcgcc
    16501 tgaaataaag cggcgatatc cactgccgat agttggtaaa gtgcgctttg tttatgtcgg
    16561 atgcgacgct aacgcgtctt atccgaccta caaaaccgtg caaattcaat atatggcatg
    16621 aaatatgtag agcgtagcgc atcaggcaat tttgctattt acgcagcact cctcgcgtaa
    16681 gaattcaatt atcgcttcca gcattgcgta ttgcgaaaca cacatcagcg tccgactctc
    16741 acggttttgt ttgaccagtc ctaccgatac cagtgctgat atatggtggc tgagtgtgga
    16801 gccaggtatc ccaagctgct tttgcaactc accgaccggc aacccttgtt ccccggcttt
    16861 gacaagatgt ttaaatatga acaaacgggt cgggtgacct agctctttca gcgctttagc
    16921 gacttcttcc aactgcatat gacacccctc tgtctggttg atatttcgat aataccagaa
    16981 atatcttgat ctgaatttca tttcgattat tctggaaata tagatttatc caatgaggtt
    17041 acgtatgagt agttggcttg cgatgctgca agatgccgca gagatgtttg tgtttctcgc
    17101 cgtcgagctt tcgttgctgt ttatagtgat tagtgccggt gtcagcctga taagacaaaa
    17161 ggtgccagac cataaaatcc agcagatgat gggggccaga aaagggagag gctatctcct
    17221 ggctgctctg ttgggagccg ttaccccgtt ctgtagttgc tcgacaatac ccatgttacg
    17281 tggattgttg tcagcgaaag ccgggt
//
LOCUS       DAEEKQ010000079        17044 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.079, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000079 DAEEKQ010000000
VERSION     DAEEKQ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.079"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            122..445
                     /gene="soxS"
                     /locus_tag="KLK57_002487"
     CDS             122..445
                     /gene="soxS"
                     /locus_tag="KLK57_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HBE0892833.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="KLK57_002488"
     CDS             complement(448..2034)
                     /gene="pdeC"
                     /locus_tag="KLK57_002488"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HBE0892834.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQI
                     TDYVIDNVFRDLGAYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVEKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            2464..2744
                     /locus_tag="KLK57_002489"
                     /pseudo
     CDS             2464..2744
                     /locus_tag="KLK57_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
     gene            complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="KLK57_002490"
     CDS             complement(2843..3379)
                     /gene="ssb1"
                     /locus_tag="KLK57_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HBE0892835.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            3633..6455
                     /gene="uvrA"
                     /locus_tag="KLK57_002491"
     CDS             3633..6455
                     /gene="uvrA"
                     /locus_tag="KLK57_002491"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HBE0892836.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(6490..6846)
                     /locus_tag="KLK57_002492"
     CDS             complement(6490..6846)
                     /locus_tag="KLK57_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBE0892837.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(6850..7266)
                     /locus_tag="KLK57_002493"
     CDS             complement(6850..7266)
                     /locus_tag="KLK57_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HBE0892838.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PLHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="KLK57_002494"
     CDS             complement(7377..8090)
                     /gene="aphA"
                     /locus_tag="KLK57_002494"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HBE0892839.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            8492..8714
                     /locus_tag="KLK57_002495"
                     /pseudo
     CDS             8492..8714
                     /locus_tag="KLK57_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="KLK57_002496"
     CDS             complement(9218..10411)
                     /gene="tyrB"
                     /locus_tag="KLK57_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HBE0892840.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(10664..11743)
                     /gene="alr"
                     /locus_tag="KLK57_002497"
     CDS             complement(10664..11743)
                     /gene="alr"
                     /locus_tag="KLK57_002497"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HBE0892841.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSIGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="KLK57_002498"
     CDS             complement(11796..13211)
                     /gene="dnaB"
                     /locus_tag="KLK57_002498"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBE0892842.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            13294..14277
                     /locus_tag="KLK57_002499"
     CDS             13294..14277
                     /locus_tag="KLK57_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HBE0892843.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREEDLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="KLK57_002500"
     CDS             complement(14443..14685)
                     /gene="pspG"
                     /locus_tag="KLK57_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HBE0892844.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="KLK57_002501"
     CDS             complement(14819..15856)
                     /gene="dusA"
                     /locus_tag="KLK57_002501"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HBE0892845.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(16219..17044)
                     /locus_tag="KLK57_002502"
                     /pseudo
     CDS             complement(16219..17044)
                     /locus_tag="KLK57_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
BASE COUNT         4423 a         4274 c         4070 g         4277 t
ORIGIN      
        1 tagcagcgct ttaatgcggg gtaatttctt ttccataaat cgctttacct caagttaact
       61 tgaggaatta tactccccaa cagatgaatt aacgaactga acactgaaaa gaggcagatt
      121 tatgtcccat cagaaaatta ttcaggatct tatcgcatgg attgacgagc atattgacca
      181 gccgcttaac attgatgtag tcgcaaaaaa atcaggctat tcaaagtggt acttgcaacg
      241 aatgttccgc acggtgacgc atcagacgct tggcgattac attcgccaac gtcgcctgtt
      301 actggccgcc gttgagttgc gcaccaccga gcgtccgatt tttgatatcg caatggacct
      361 gggttatgtc tcgcagcaga ccttctcccg cgttttccgt cggcagtttg atcgcactcc
      421 cagcgattat cgccaccgcc tgtagtttta ttgcccgcgc gttaactccc gcgcgggtaa
      481 ttgctccatc cattgcataa acacctgcgg cggcatcgcc ttcgcaaaaa accatccctg
      541 gcaatagcgc acgccgcgtt tgcgcagcca gttaacctgc tcctcagttt cgacgccttc
      601 agcgatcgtt tttaacccca ggctgtgcgc cagctcgatg atgtgttccg caatcaaatg
      661 actggttttg tgggtggtca gcgtttcaac aaacgattta tcgattttca aaatatcgac
      721 attcaatgat ttaaggttat gcaagttaga gtagccaata ccaaaatcat caattgccac
      781 ttcgtaacca gcctggcgga aagccagaat aatcggcgtc attttttcga catcaagaaa
      841 tgcatgctca gtcacttcaa atttaatttg ctgcggacgc accgcgtatt gctctgtttt
      901 ctgattgatt cgcgctatca accgtgacgt atgaaaatcg gaggccgaca ggttaataga
      961 aacatagcga tcggcatgtg ttgccaagta cgcgcccaga tcgcggaaga cattatcaat
     1021 aacataatca gttatctgtt ctatcatccc ctccttttct gccagcggaa taaactctgc
     1081 cggattcatt atttgcccct gctcaccaag ccaacgtaac aacgcttcag cgccgataca
     1141 tttttctgtt ttgatatcga ttattggctg gtaataaaga caaagttgat gtttttcgag
     1201 ggcgcgttgc aatttacgtt tgggagataa atagttttgt cgaatacgta gccagagtaa
     1261 tagcagaaca agactcccca aaataccggc gggcaacgca aaaataagat gattataaaa
     1321 atgggttata agacgttgat atgaagtcgc aacaatggct gcaattgggc gttttgtcga
     1381 ataaactgtc gcatataaat agccatttct ttgtacggtt aaatccttca aatgaatcag
     1441 cggagaaaac gttgctgccg aggcctcttt gcttaacgag aaaaaggttt tcgtcaccgt
     1501 atcatacaca ccccattgca atgtcgggtc atcagacatc acttcactcc agaagagagg
     1561 gttgataacc gccacataat ttccccgctg catataggtc attttatagc cagagaaaaa
     1621 aggcgtatcg cggtaataat agatagaaac gttaggttca cgcttataat cggccggtgc
     1681 aatcgtatag ccgtttacag gcgctatcag cgatgagcat aaaaaatggt tatcacgggc
     1741 atagatcaat tcattaatat aaagatagcc acgaataata ttcaacattc gcttttgatg
     1801 ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta aatctgcctg
     1861 gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga gctgattatt
     1921 cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg gaaagagaac
     1981 taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac tcattgctat
     2041 caatttcctg cctgtttata cccggccatt gcaaggtgaa aaccctgggt accgacgtct
     2101 ttagataaat cttacggtat aaatagcact tttgcctgat ttgaaattga tacctttcta
     2161 atcgtcacct ggatgaaaat aattcattga tattcaaaat taatttccta caggaatgca
     2221 gaggcggcgg gaagatatgc ccttaatgcc actgaaaaac cacataatct gtacagcaat
     2281 aaaagtcacg gcctaatatg gctaacaaac aatcaattgt gatatagctc acaaaattaa
     2341 tgaaacaaac agagtgtttc atttttgtgt tctgcaagcc ccgcgatagc cagagtgtac
     2401 cacgcctccc gtgaacaacg ccgcgctgtc cagggcatcg gctcttttca gaggaattgt
     2461 ttgatggcta ccctcaccac tggcgtggtt cttcttcgct ggcagcttct tagtgccgta
     2521 atgatgtttc tggccagcac atcaacatcc gttttcgtcg gtctgattat gtcgggcttg
     2581 cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg gggttgcttg
     2641 gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg atgatagaag
     2701 agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta gaagcctcca
     2761 taacggaggc ttctttttta tggctggcag atgctttaat catccacctt aaaacaatat
     2821 aacctattgt tttaatgaca aatcagaacg gaatgtcatc atcaaagtcc atcggcggct
     2881 cgttagacgg cgctgccgga gcggactgct gcgggcgaga ctgcgcgccg ccgctgaact
     2941 gattgccacc ctgcggctgc tgaggctgcc cccaaccgcc ctgcggctga ccaccaccga
     3001 tattaccacc tgccggagcg ccaccaccct gacgaccacc cagcatctgc atggtgccgc
     3061 caacgttcac cacgacttct gtggtgtagc gatcctgacc ggattgatcg gtccatttac
     3121 gggtacgcag ctgaccttcg atataaacct gagaaccttt acgcagatat tcgctggcca
     3181 cttctgccag tttgccgaac agcacaacgc ggtgccattc agtctgttct ttcatctcgc
     3241 cggtcgcttt atcacgccag gattcggaag tagccagcgt aatgttggca actgcgccac
     3301 catttggcat gtagcgtact tccgggtcct gacccagatt accaacgaga ataaccttgt
     3361 ttacgcctct gctggccatg ttcgtgtctc ctgaaaaaaa tcgttctgaa taagtgtaaa
     3421 cgcgcgattg taccattacc aatagcgctt ttactatgtt gtgacctcgg ttccgggaaa
     3481 caaacctggc cagacattgt tacacaacac tccggataat gcattccaat actgtatatt
     3541 cattcaggtc aatttgtgtc ataattaacc gtttgtgatc gccggtagca ccatgccacc
     3601 gggcaaaaaa gcgtttaatc cgggaaaggt gaatggataa gatcgaagtt cggggcgccc
     3661 gcacccataa tctcaaaaac atcaacctcg ttatcccccg cgacaagctc attgtcgtga
     3721 ccgggctttc gggttctggc aaatcctcgc tcgctttcga caccttatat gccgaagggc
     3781 agcgccgtta cgttgaatcc ctttccgcct acgcgcggca gtttctgtca ctgatggaaa
     3841 agccggacgt cgatcatatt gaggggcttt ctcctgccat ctcaattgag cagaaatcga
     3901 cgtctcataa cccgcgttct acggtgggga caatcaccga aatccacgac tatttgcgtt
     3961 tgttgttcgc ccgcgtcggc gaaccgcgct gtccggacca cgacgtcccg ctggcggcgc
     4021 aaaccgtcag ccagatggtg gataacgtgc tgtcgcagcc ggaaggcaag cgtctgatgc
     4081 tgctcgcgcc aatcattaaa gagcgcaaag gcgaacacac caaaacgctg gagaacctgg
     4141 caagccaggg ttacatccgt gctcgtattg atggcgaagt ctgcgatctt tccgatccgc
     4201 cgaaactgga actgcaaaag aaacacacta ttgaagtggt ggttgatcgc ttcaaggtgc
     4261 gtgacgatct tacccaacgt cttgcggagt cgtttgaaac cgcgctggag ctttccggtg
     4321 gtacagcggt agtggcggat atggacgacc cgaaagcgga agagctgctg ttctccgcca
     4381 acttcgcctg cccaatttgc ggctacagta tgcgtgaact ggagccgcga ctgttttcgt
     4441 ttaacaaccc ggcaggtgcc tgcccgacct gtgacggcct tggcgtacag caatatttcg
     4501 atcctgaccg cgtgatccaa aaccccgagc tgtcactggc tggcggtgcg atccgtggct
     4561 gggatcgccg caacttctat tatttccaga tgctgaaatc gctggcagat cactataagt
     4621 tcgacgtcga agcgccgtgg ggcagcctga gcgcgaacgt gcataaagtg gtgttgtacg
     4681 gttctggcaa agaaaacatt gaattcaaat acatgaacga tcgtggcgat acctccattc
     4741 gtcgtcatcc gttcgaaggc gtgctgcaca atatggagcg ccgttataaa gagacggaat
     4801 ccagtgcggt acgtgaagaa ttagccaagt ttatcagcaa tcgcccatgc gccagctgcg
     4861 aaggaacgcg tctgcgtcgg gaagcgcgcc acgtgtatgt cgagaatacg ccgctgcctg
     4921 ctatctccga catgagcatc ggtcatgcga tggaattctt caacaatctc aaactcgcag
     4981 gtcagcgggc gaagattgca gaaaaaatcc ttaaagagat cggcgatcgt ctgaaattcc
     5041 tcgttaacgt cggcctgaat tacctgacac tttcccgctc ggcagaaaca ctttccggcg
     5101 gtgaagccca gcgtatccgt ctggcgagcc agattggtgc gggcctggtt ggcgttatgt
     5161 atgtactgga cgagccgtct atcggcctgc accagcgcga taacgagcgc ctgttgggta
     5221 cgcttatcca tctgcgcgat ctcggtaata ccgtgattgt ggtggagcat gacgaagacg
     5281 caattcgcgc cgctgaccat gtgatcgata tcggtcctgg tgcgggtgtt cacggcggtg
     5341 aagtggtcgc agaaggtccg ctggaagcga ttatggcggt gcctgaatcg ttgaccgggc
     5401 agtacatgag cggtaaacgc aagattgaag tgccgaagaa acgcgttccg gcgaatccag
     5461 aaaaagtgct gaagctgaca ggtgcacgcg gcaacaacct gaaggacgtg acgctcacgc
     5521 tgccagtcgg tctgtttacc tgcatcacag gggtttcagg ttccggtaaa tcgacgctga
     5581 ttaacgacac actgttcccg attgcccaac gccagttgaa tggtgcgacc atcgccgaac
     5641 cggcaccgta tcgcgatatt caggggctgg agcatttcga taaagtgatc gatatcgacc
     5701 aaagcccaat tggtcgtact ccacgttcta acccggcgac ctataccggc gtgtttacac
     5761 ctgtgcgcga actgtttgcg ggcgtaccgg aatcccgtgc gcgtggttat acgccaggac
     5821 gtttcagctt taacgtccgt ggcgggcgct gcgaagcctg tcagggcgac ggtgtgatca
     5881 aagtggagat gcacttcctg ccggatatct acgtgccgtg cgatcagtgc aaaggtaaac
     5941 gctataaccg tgaaacgctg gaaattaagt acaaaggcaa aaccatccac gaagtgctgg
     6001 atatgaccat cgaagaggcg cgtgagttct ttgatgcggt gccagctctg gcgcgtaagc
     6061 tgcaaacgtt gatggacgtt ggtctgacgt acattcgcct ggggcagtcc gcaaccacac
     6121 tttctggtgg tgaagcccag cgcgtgaagc tggcgcgtga gctgtcaaaa cgcggcaccg
     6181 ggcagacgct gtatattctc gacgagccga ccaccggtct gcacttcgcc gatattcagc
     6241 aactcctcga cgtgctgcat aaactgcgcg atcagggcaa taccattgtg gtgattgagc
     6301 acaatctcga cgtgatcaaa accgctgact ggattgtcga cctgggacca gaaggcggca
     6361 gtggcggcgg cgaaatcctc gtctccggta cgccagaaac cgtcgcggag tgcgaagctt
     6421 cgcatacggc acgcttcctc aagccgatgc tgtaatcgtt aaggccgctt tctgagcggc
     6481 cttttccttt cagagttgta ccagcaattt acgcttttct tccggtagta aattcactgc
     6541 ctgctgataa gacgcatcca ccagataata gatttgcgaa tccggcagcg aaccgtcgag
     6601 atacacggtg ctccagtgcg ctttattcag atggcggcta ggacgcacat cgctgtgctg
     6661 ctgacgtagc agctccgcca gctccgggct ggttttcagc gaaacagctg ggcgattttc
     6721 tacttctttc accatcgcaa acagtacatc ttccactttg atctgcgtcg ctttccagtc
     6781 gttatgcacg ctctgttctg cgcctggttt tgccatgcaa tattgtagca actccgaaat
     6841 ggtcattttt tactcccctt gtagtgtcgc gatgatgcga cgcgatccgc cgtggatgcg
     6901 atgttccccc agccagatgc cttgccaggt gccggtctga atacgccctt tatgtaacgg
     6961 caatacaagc gatgttccca gcattgagga tttgatatga gaaggcatat cgtctgctcc
     7021 ctcatagtca tgctcataat ttccgttgtc gggaacggtg cggaggaaaa aacgctccat
     7081 gtcgtggcgt acggtgggat cgcagttctc attaagtgtc agagaggcgg aggtatgttg
     7141 cagcaacaga tgcagtaagc cgatgttaac gcgcggcata tcagccagct gattcagaat
     7201 ttcatccgtt accagatgaa acccacgaga tttggcgcta agcgtgagcg tcttttgata
     7261 ccacatgtgc tgctccttga taaaactctc ttaatcagtt tgcagcaaga cagcgaaagg
     7321 ataaaggtgt gattaaaaaa acagcattcg ggagagcatc acgctctccc gctctgtcag
     7381 tattctgaat tgacgatcac ctcttcacca aacgcacccg cttgtggcaa gggtttgtag
     7441 gtagagttgg aggcgcgcag aatgcggata ccacgagcgc cgacatcgcg tgcggcggta
     7501 atatcgttat cggaatcgcc ataaaaaatt cggatatttt tatcctgcag ccattgcgat
     7561 tttgtatttt gccctggttt atcgcccgca aagattaccg gattcatgtt ggtggcagga
     7621 atatgaaaat tatccgccag cgtttttgaa accgtttctg ttttcgtcgg gctacgacca
     7681 gtcacaaaga agatcgcgtc accgcggcgt acatgcatat caatcagctg gcgagcgacc
     7741 tcttttggaa tgctgaattc atcccagcca ttattcattt tttcccagaa cacaggattt
     7801 ttcagataat cttcgctttc tggcgagaag gtttttttgc cgcgccagaa ccccggactg
     7861 gaaaaaagta ccgtgtcatc gatatcaaac cccaccgcca ttggcggacg ccctgcgagg
     7921 ctattttcaa tttgtgcgac cgaaacccaa tgaattggtg cctgttcagc aagcctggca
     7981 acgttagtcc cagggttaag cggtgaagga gatgaggcca gggcaacagc ggaactgttt
     8041 agagcgaaca ataagcaaac ggcactgatt gcctgtgtga tcttgcgcat atttttccct
     8101 aaatagtcag tatgttgaaa ctttttattg tgagatttgt tgcaaaaacc atctgaccat
     8161 aacgccagct gccggggaaa ggaaggatta tctgcggttt ttgtgatgaa aagcaggatg
     8221 attatattta acccagtaaa tacgttcctg tctttttttg tataaaaaat gtttattact
     8281 cgatgtaagg atacattaat tttacgttta ttatgatgaa tcctctgcaa atgagcgaaa
     8341 tttgaaagaa catttatctg ctgtacgatt taatgaactt aatgatacag cattacaaaa
     8401 acaatcgaag tttataaaga tgattcctga ttgaccagaa ccctatccta caatggtgac
     8461 taattttatt actcccgatg gagatgatga tatgaatata tcatatgtga atagcaacaa
     8521 aacgacatct ttacctgttg agctagatgc actaaataat aaagacatct cttatgccaa
     8581 agatttttct tatgctaaag atttcttttt atacatagaa acacagttaa aaattgcaaa
     8641 agatttttgt agacctggag aagaagtatc aagttctatt gcaagtaaag tttttcacgc
     8701 atttatcgat ttagttaaca aaatcagggg taagaaagac tttatgtata tttgcacgct
     8761 ttgctgtttt gccgaggagg ttaaaggtga ttattctcat tacaggacct ttttatttga
     8821 tattggtaat caatataagg ttaaacttac acaaagcgga aaaaaagagt tctctttaac
     8881 attagaattt aacgatacta taattgaatc tcagaaagtc acaggcaata aggcaaagta
     8941 tattcttgag gatatagaaa tattctatcg taacaaaccc gatacttatt attaaattta
     9001 ataatggtta gatgtgcaga aattattact ccacacgaaa aaatagggaa aggaattcca
     9061 gataattggg ataaacttaa caataaaaga actgacaacg ttttattatc tctcacgtag
     9121 aacaacggca tcaaaaaaat catctacaaa gaaatagtga aagcgacgac agtttaattt
     9181 cactgcaggc tgggtaactc cagcctgctt tcctgcatta catcaccgca gcaaacgcct
     9241 ttgccacacg ttgtacattt gccgtattta acccggcgac acacatgcga ccgctggcga
     9301 tgagatagac accaaattct tcacgtagtc ggtcaacctg agcggcactt aaaccggtat
     9361 aactgaacat gccgcgctga ttaagcagat aatcgaaatt gcgttctggc atctctgtgc
     9421 ttaatacctt caccaattcc tgacgcattg ccagaatgcg agtacgcatc tcttctactt
     9481 ccgccagcca gctggctttc aatgcctcgt cattcagcac tgcagccacc acctgcgcac
     9541 caaaattcgg cgggctggag tagttgcggc gaactgttgc tttcaattgc cccagtacgc
     9601 ggccagcggc ttcggcatct tcacacagaa cagaaagtcc gccgacgcgc tcgccgtaaa
     9661 gggagaaaat tttcgagaac gaattgctca ccagagcggg taatccagcg ctggcaatgg
     9721 cgcgaatagc gtaggcatcc tcttccatac cggcactaaa tccttgatag gcaatatcga
     9781 ggaatgggat aagctcgcgg gctttgagaa tttcaatcac cgcatcccac tgatcattag
     9841 tgagatcggc acccgttggg ttgtggcaac atggatgcag caacacaata ctgcgggcag
     9901 gtaatgtttt cagcgtcgcc aacaggtcat taaagcgcac gctgttagtc gcttcgtcat
     9961 accaggggta agtactcact tcgaatccag ccccggcgaa tattgctacg tggttttccc
    10021 aggtaggatc gctgacccag acgcctgatt ccgggaagta gcgtttcagg aaatccgcgc
    10081 ccactttcaa tgcccctgag ccgccaaggg tttgaatggt tgctacgcgc tgttgttgca
    10141 gtaccggatg gtccgcacca aacagcagcg gcgcaatggc atggcgatag ctgttaagcc
    10201 cttccatcgg taaataaagc gaagcgccat ggggctgcgc attcaggcgc gcttccgcct
    10261 ccgccacggc tttcagttgt ggaataattc cgtcttcgtt gtagtacaga ccgatactta
    10321 aattcacttt gtcgctgcga gggtcttctt taaaacgctc cataagcgta agaatcgggt
    10381 cgccagcgta ggcgtcaact ttttgaaaca cgcgatggtt ctccaggttt acgggcaggt
    10441 ggttaaaaca caataaaccg gaagaaggcg aagatcgagt ggatgttcag gagcgaacgg
    10501 caattagcaa cagagtgaga ctcatgacaa acgaacatcc gccagaggca cgacctttat
    10561 aagagcatga aagaacatca acttattgaa tttttaggat tttattggcc ggataaggca
    10621 ttcacgccgc atccggcaca gacaatcaaa tattacagaa cgattaatcc acgtatttca
    10681 tcgcgaccct tgaagtcagg cgcgtaataa gttcgtaagc gctcactttt gtcatttcag
    10741 cgatacgttc tacgggcaaa ccttcgcccc ataaaatgac cggatccccg gctttgtcct
    10801 gcgcctgtgg acctaagtct acgcagatca tatccatcgc cacgcgtccg acaatcggta
    10861 cttcgcgacc gttcaccagc actggcgtac cggatggagc ggcgcgcgga taaccatcgc
    10921 catagcccat cgcgactacg ccaagacggg tatcacgttc gcttacccag gttccaccat
    10981 aaccaacagg ctctccggct ttatgctcac gcacggcaat caggctggag gttagtgaca
    11041 tcactggctg acagccaaaa tcggcaccga tggagcgatc ttccagcggc gagacgccat
    11101 aaagaatgat gcccgggcgc acccagtcaa aatgcgattg tggccacagc agaatgccac
    11161 ccgacgcggc aatggaacgt tgaccaggtt tgccttcgca aaaggtatta aagatagcga
    11221 gttgtttctc ggttgcgcca cattttggtt catccgcgcg cgcaaaatgg ctgacgatat
    11281 tcaccggctg acgaacgttt ttgcactgtg tcaggcgatg ataaaacgcc tcagcctgtt
    11341 ccggccttac gcccagacgg tgcataccgg tatcgagttt catccagacg gtaaccggct
    11401 cgtccaggct agcctcttcc agcgcagcca gctgttcttc gttatgcacg gcggtatgaa
    11461 aatgttgcgc agaaatcgtc ggcagatctc tggcatcaaa aaagccttcg agtaacagta
    11521 caggtttggt gattcccccc gcacgcagtc gcagagcttc ttcgagacgg gctacgccaa
    11581 aggcgtcagc atcggggagc gttcgcgcgg tctcaagaag accgtgacca taagcgttcg
    11641 ctttcaccac cgcaaccatt ttactggcag gggccagttc acgaagacgt tgcaggttgt
    11701 gtcgcagagc gcggcggtta atcacaacag ttgccgcttg catttgtgtt ccttgataag
    11761 tgtttgcttt aattacctaa ttcataaaat aattattatt cgtcgtcgta ctgcggcccc
    11821 gcatagttgt cgaagcgcga ccattgaccg ttaaaggtca ggcgtaccgt cccgattggg
    11881 ccgttacgtt gtttaccgat aataatttcc gcgatgcctt ttaaatcact gttttcgtga
    11941 tacacctcat cacgatagat aaacataatc aagtccgcat cctgctcgat agagccagat
    12001 tcacgcaggt cggagttgac cgggcgtttg tcggcacgtt gttccagaga acggttcaac
    12061 tgggacagcg ccaccaccgg cacgttcagt tctttcgcca gtgctttcag cgagcgagag
    12121 atttctgcaa tttccagcgt acggttatcg gaaagcgccg gtacgcgcat cagttgcagg
    12181 tagtcgatca tgataagccc gatgccgccg tgttcacggg caatacggcg tgcgcgggaa
    12241 cgcacttccg ttggcgtcag gccggaggag tcatcgatat agatattgcg tttttcgagc
    12301 aaaataccca tggtgccgga aatgcgcgcc cagtcttcat catcgagctg cccggtacgg
    12361 attttagtct ggtcaacgcg cgacagcgac gccagagaac gcatcataat ctgttctgaa
    12421 ggcatctcca gcgagaagat aagtactggt ttatcctgca acatcgccgc gttttcgacg
    12481 aggttcatcg caaatgttgt tttacccatc gacggacgcg cggcgacgat gatcaaatcc
    12541 gacggctgca agccagcggt ttttttgttg agatcgtcat aaccggtgtt tactccggta
    12601 acgccatcgt gtggctgctg aaacaactgc tcaatacgcg ccacggttgc gtcgagcaca
    12661 tcggcgatgt tcttcggccc ttcgtctttg tttgcacgac tttcggcaat tttaaagacg
    12721 cgggattcag caaggtccag cagatcttcg ctggtacgcc cctgcggatc aaaaccggct
    12781 tcagcaatct cattcgcaac cgagatcatc tcacggacaa cggcacgttc acgcacgatg
    12841 tcagcataag cactgatgtt cgccgcactt ggcgtatttt ttgacagctc tgccagataa
    12901 gcaaaaccac cgacgctatc gagttgcccc tggcgttcca gcgattccgc aagggtaatc
    12961 agatcgatag gactaccgct ttcctgcaaa cgcgccattt cagtaaagat atggcggtgt
    13021 gggcgggtgt aaaaatcgtc tgccaccaca cgctcggcta catcgtccca gcgttcgtta
    13081 tccagcatta aaccgcccaa caccgactgc tccgcttcga tcgagtgcgg aggcactttc
    13141 agcccggcaa cttgtggatc gcgttcgcgg ggttcagcct gctgtttgtt gaagggttta
    13201 tttcctgcca tagtgaatgg agttaccgag ataaagaatg ggtcgaaact ttaccatatg
    13261 aagcagaccc tgacgatacg ttctggagga cacatggcaa cacgaattga atttcacaag
    13321 cacggtggcc cggaagtact tcaagccgta gagttcactc ctgccgatcc ggcagagaat
    13381 gaaatccagg tcgaaaataa agccatcggc atcaatttta tcgacacgta tatccgcagc
    13441 ggcctttacc cgccgccatc actacccagc ggattaggta ccgaagcggc aggcatcgtg
    13501 agtaaagtcg gcagtggtgt aaagcatatt aaggcaggcg atcgtgtagt ctatgcgcag
    13561 tcggcgttag gcgcttacag ctctgtgcat aacattaatg cggataaagc ggcgattctg
    13621 cctgcggcaa tttcttttga gcaagctgcg gcatccttcc tgaaaggctt aacggtttat
    13681 tatctgctgc gcaaaaccta tgaaattaaa cccgatgaac agttcctgtt ccacgcagcg
    13741 gctggcggcg ttggcttaat tgcctgccag tgggcaaaag ccctgggcgc gaaacttatc
    13801 ggcaccgtag gaaccgcgca aaaagcgcag agcgcgctaa aagcgggcgc gtggcaggtt
    13861 attaactatc gtgaagagga tctggtcgag cggttaaaag agatcaccgg cggtaagaaa
    13921 gtgcgcgtgg tgtacgattc cgtgggcaga gacacctggg aacggtcgct ggattgcctg
    13981 caacgccgcg gcttaatggt cagttttggc aactcatcag gtgcggttac cggtgtgaac
    14041 ttaggcattc tcaatcaaaa aggctcgttg tatgtgacac gcccttccct gcaaggctat
    14101 atcaccacgc gggaggaatt aaccgaggcc agtaatgaac tgttctcttt gattgccagc
    14161 ggtgtgatta aggtcgatgt cgccgagcag cagaaatatc cgctgaagga tgcgcagcgt
    14221 gcgcatgaga ttctggaaag ccgggcgacg caaggttcca gcctgttgat tccataaaag
    14281 aaatagggct tccacctggg aagccctttc tttttatagt tcggctgtat gtagggtaca
    14341 gcacgatgaa tctgttagag gcgcaatagt gacagatttg attatcaatt cctattttgt
    14401 tctaaggata aaaccttagg ttgtgatcat ccgcacaatc ccttagtaac gccagcggtc
    14461 ataacgctga tatttcggca cttttggtgc tttaatcgcc ttaataaccc acaccaccgc
    14521 aatcgccagt agtaaccacg gcagcaactt aatcatcaat gccagcatac cgccgaggaa
    14581 cataatggcc gtcgccacaa ccagcgcggc gataatgccc agcaacgaaa cgccggtgac
    14641 catcagcatg acaaaaaagc caatcacaaa aagtagttcc agcatgatgc tctcccaaat
    14701 atgaaatctc ttgctggcat tacaagaatc atgccaaaaa taatctattg atttaacagc
    14761 aaaacgcccc gcgacggtgc gcagggcgtg gtgaatttga ctactttttg gtgaaaagtt
    14821 aacgcttatc cgccaccagt ttgagcgcgt gttccagcac attaatgtct gcacccgctt
    14881 tatgggcatt ttcacttaaa taacgccgcc actgccgcgc gccaggaata ccctggaaca
    14941 agcccaacat atgccgggta atatggccga gatacgtccc ctggctgagt tcacgctcaa
    15001 tgtacggata catggcgcgc actaccgcca ccggatcggc atcggtatcc gaggaaccaa
    15061 agatctcccg gtctaccgcc gccagaatac ccggattctg atacgcctcg cgcccgacca
    15121 tcacgccatc catatgttgc aggtgtgctt tggcctcttc cagcgacttg ataccaccgt
    15181 taatcgacat tgtcagatgc ggaaagtcac gcttcagttg atacacacgc ggataatcga
    15241 gcggcgggat ttcacggttt tctttcgggc ttaaccccga aagccaggct ttacgtgcgt
    15301 ggatgataaa catctcacac tcgcctttgc cggaaacggt gttgatgaaa tcgcagagaa
    15361 attcatagct gtcctggtca tcgatgccaa tacgcgtttt caccgtcact ggaatcgaca
    15421 ccacatcgcg catcgctttc acgcagtcgg caaccagctg cgcattaccc atcagacacg
    15481 caccaaacat gccgttctgc acccggtcag acgggcagcc gacattcagg ttgatctcat
    15541 catatccacg cgcttctgcc agcttcgcac actgtgccag cgccgccgga tcgctaccgc
    15601 cgagttgcaa cgctaccgga tgttcttctt cactgtacgc caggtaatca cctttaccgt
    15661 gaataatcgc ccctgtggtc accatttcgg tatacagcaa cgtattgcgg gaaagcagac
    15721 gcaagaaata gcggcaatgt ctgtccgtcc agtcgagcat tggtgcaacg ctaaaccgac
    15781 cactccagtg aacgtcagtt ttttcaggca ttgcgctggt ttggttgatt ttttgcattt
    15841 cagaattacc gtgcattttc aaatgtagag attattttat cgatatatca tggggttatg
    15901 ttattcagca tcactattca ggaggctcaa tagcggggta ctataccata acaacaggaa
    15961 gcgcctgtct cattgcaaaa gaaaattgag atcctctcaa ggcatgaagc tctcacgaag
    16021 tgatggaaat aatcttatta gccgttaact ttgttaaggc caatgataaa caatccaggt
    16081 tcgacgataa ataaaaaatc acacattaaa ctctggtgat atatctccct gctaatagca
    16141 ttgatagaga aaaaagaacc caataaatat tgggtccttt tatataatgc cttccatact
    16201 atcgaagaac ttcacatatt atttctccga tttaaccccg aacaaatcat aaattaattt
    16261 agaagtgtct gtaagtattt taatctcttc ctttgaggtt gggtcaaacg acttcgcgaa
    16321 gctaattaat tgtggtgcag catctcgcat tttgcttaaa atatcaggat cgagcgttcc
    16381 ttcattcacc agatgtgaca tcttatccag atagccatca aaattcattt ctcccccatc
    16441 agccagacgc ttcatctctc ccaggtactt cttcatatca cgttgagata atttttcaaa
    16501 ctgagctttc aggtagctct cattattttc attaatatac actgtttctg ataaatctgc
    16561 gtaggcacta gaatatgaaa gtgaaattaa cagtgaagca aataatttta agctgttgtc
    16621 atcatgttcc tggcaggcat taacaaacgt taaaaaacca gaacctattg cttgaaaatg
    16681 tatattcgct aaaggttcat tatccttaga ttcctcataa aatagctgaa ccgtggaggg
    16741 aaggccattc ttagtttcta catcaaaagt acattgttca atgggagtcg catctttatt
    16801 atccccagga ttacatatac tattaatcgc gaaatgaaaa atagcccggt caacaactga
    16861 tgcaataaag tcacgatcgt ttaaatcctc atcagtgcaa tcgtcaatat acttattgac
    16921 ccacattttg agatcattta tttgctcaat taaaaacaca tttttttacg tgcatgccat
    16981 aaattatgcg tgattcgttg aataaacaaa tctatttttg caataattat atcccgatga
    17041 cgca
//
LOCUS       DAEEKQ010000080        16847 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.080, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000080 DAEEKQ010000000
VERSION     DAEEKQ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16847
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.080"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            92..1132
                     /locus_tag="KLK57_002503"
     CDS             92..1132
                     /locus_tag="KLK57_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HBE0892846.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLHTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="KLK57_002504"
     CDS             complement(1164..2357)
                     /gene="lplT"
                     /locus_tag="KLK57_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HBE0892847.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETMSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(2350..4509)
                     /gene="aas"
                     /locus_tag="KLK57_002505"
     CDS             complement(2350..4509)
                     /gene="aas"
                     /locus_tag="KLK57_002505"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HBE0892848.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            5095..6126
                     /gene="galR"
                     /locus_tag="KLK57_002506"
     CDS             5095..6126
                     /gene="galR"
                     /locus_tag="KLK57_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HBE0892849.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLVVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="KLK57_002507"
     CDS             complement(6133..7395)
                     /gene="lysA"
                     /locus_tag="KLK57_002507"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HBE0892850.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            7517..8452
                     /gene="lysR"
                     /locus_tag="KLK57_002508"
     CDS             7517..8452
                     /gene="lysR"
                     /locus_tag="KLK57_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HBE0892851.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(8439..9086)
                     /locus_tag="KLK57_002509"
                     /pseudo
     CDS             complement(8439..9086)
                     /locus_tag="KLK57_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase"
     gene            complement(9217..10635)
                     /gene="araE"
                     /locus_tag="KLK57_002510"
     CDS             complement(9217..10635)
                     /gene="araE"
                     /locus_tag="KLK57_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HBE0892852.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="KLK57_002511"
     CDS             complement(10950..11711)
                     /gene="kduD"
                     /locus_tag="KLK57_002511"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HBE0892853.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIKQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQRNGGKIINITSMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPIVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(11741..12577)
                     /gene="kduI"
                     /locus_tag="KLK57_002512"
     CDS             complement(11741..12577)
                     /gene="kduI"
                     /locus_tag="KLK57_002512"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HBE0892854.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(12864..14045)
                     /locus_tag="KLK57_002513"
     CDS             complement(12864..14045)
                     /locus_tag="KLK57_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HBE0892855.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            14300..15529
                     /locus_tag="KLK57_002514"
     CDS             14300..15529
                     /locus_tag="KLK57_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBE0892856.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSACEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTYLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
     gene            15989..16621
                     /locus_tag="KLK57_002515"
     CDS             15989..16621
                     /locus_tag="KLK57_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0892857.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNTAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT         4448 a         4417 c         3890 g         4092 t
ORIGIN      
        1 ccggctggcc ttaagatttt tctctttccc ttttcccttc cctctgccat ttttatattc
       61 cttatgtcgt gattataaaa aggaaacggc tatgcaatat caccgtatac cccacagttc
      121 gctggaagtc agcacgctgg ggcttggcac gatgacgttt ggtgaacaga acagcgaagc
      181 cgacgcccac gcacaactcg actatgccgt cgctcagggc attaacctta tcgacgttgc
      241 cgaaatgtac ccagtacctc cgcgccccga aacgcaaggg ttaaccgaaa cctacgtcgg
      301 caactggctg gcgaaacatg gcagccgcga aaagttaatt atcgcctcca aagtgagcgg
      361 accgtcgcgc aataatgaca agggcatccg cccggatcag gcgctggatc ggaagaatat
      421 ccgcgaagcg ctgcatgaca gcctcaagcg cctacacact gattacctcg atctttatca
      481 ggtgcactgg ccgcagcgcc cgaccaactg cttcggcaaa ctcggttata gctggacgga
      541 ttctgcgcct gcggtttcgc tgctggatac gctggacgca ctggcagagt accaacgcgc
      601 gggaaaaatt cgttatatcg gcgtgtcgaa cgaaactgca tttggcgtaa tgcgctacct
      661 gcatctggcg gacaaacacg atctgccgcg tattgtcacc attcagaacc cttacagtct
      721 gttaaaccgc agttttgaag taggtctggc agaagtaagc cagtatgaag gggtcgaact
      781 gctggcctat tcgtgcctag gtttcggcac gctgaccggg aaatatctca atggtgcaaa
      841 acccgctggc gcacgtaata cgctctttag tcggttcacc cgctatagcg gtgagcaaac
      901 gcaaaaagcc gtcgcggcgt atgttgatat cgccagacgt catggcctag accctgctca
      961 gatggcgctc gcgtttgtac gccgtcaacc gtttgttgcc agcactctgc tgggcgcaac
     1021 cacgatggat cagctgaaaa ctaacatcga aagtttgcat ctggagttaa gcgaagacgt
     1081 attagctgaa attgaagcgg tgcatcaggt ttatacttat ccggcaccat aagattaacg
     1141 ccggttaaaa ccggcgttaa atattaatga cggcgctgcc agatccacag cgccgttatt
     1201 gccagcgcaa acagcgcacc gaagccaatg ccaatgggca cgaccgggat gcctaccatt
     1261 accgccagcg agtaaatgcc cagcatcaac aacatggcgc tgttttcgcc aaggttttgt
     1321 actgcaatcg cattccccgc cccgacgctt tttttacccc gctcctgtag caacgcattg
     1381 agcggaacga caaaaaagcc ccccatcacg ccaatcagca tcaacaaggc ataggctggc
     1441 agcagctcgt gttgcaggga aaaaatcagt accaccacgc caatcaaaat cccggctggc
     1501 atacagcgtg acatggtttc cagcgtaact aacttcgctg ccgcacctgc gccaaccacg
     1561 ataccaatcg ctaccatcgc gttgagatag gtgggcgtag cgttatcggt aatgcccagc
     1621 gccaccggta cccacagcac caacaggaaa cgcagcgtga cacccgctcc ccagaataaa
     1681 ctggtgccca ccagcgaaaa acgcgtttca ccattgcgcc atagcgaggt gcaggcattc
     1741 aggaaactgc gggtcatgtt gatgagattc caggactgcc ccggacgcgc cgccgccagt
     1801 ttgggaatgt agatattggc aacgaccgca ccaccgtagg ccagtgcgca tgcggccagg
     1861 gcgacgagga catgccagtc agccagcaca ccaccggcta cggaaccgag caaaatcgcc
     1921 gctatggtag aagcttccat taaaccgtta gctttcacta acttactacc cgtggttaat
     1981 tcgccgagaa taccgtattt cgccggtgaa taggctgcag caccaacacc caccagcgta
     2041 tagccgagaa acggattgat accaaagcag atactggctg cgcccagcag cttcaggccg
     2101 ttggcaaaca tcatcacccg gcctttggcg aagctatccg ccacctgccc gacaaacggc
     2161 gcaaaaagaa tgtaagcacc tacaaacacc atttgcagga tgggctggct ccactccgga
     2221 tagaactgcg ctttcagtaa cgccagagtg gcaaacagta gggcattatc gccaaacgca
     2281 gagagaaact gcgccacgat aaccgctttc atccccttcg accacaacga agtgttagtg
     2341 tgcactgact cactcatcgt gttgttccgc ttcgtctacc cagcttttca acgtgacaaa
     2401 gtcaggtttg ccgctgccaa gtaatggcat ctgtttcaga tagcgaatat cgcgcggtac
     2461 agcaagctcc ggtacgccgt gctcgcgggc atactgttgc aacttatcgc gcgtcagttc
     2521 gttatctgtg gtgaaaagca ccagtgcctc gcctttgctg gcatcgctct taatcgcagt
     2581 ggcatggact ttatctggcg aaacaccaag tgccagttgt tccaccattt ccagcgacac
     2641 catttcgcct gcaattttgg caaagcgttt tgcgcggccc tgaatctgca caaagccctg
     2701 ctcgtcaaaa cgcacaatat cgccagtgtc ataccagccg cgctccattt cgccgcgaac
     2761 attctcggcg gtgggcactt ccagtacacc tggcttctcc acccgcagat agccgttcat
     2821 tatgttcggc cctttcagtt gcaggcgtcc gccctcttcg ataccaggga ccgacaacag
     2881 gcgcgcatcc attcctggta gaatacgccc taccgtaccg ggtttcgccg ccatcggtac
     2941 gttgatagaa acgacaggcg cgcattcggt cacgccgtag ccttcaagga tgcgcaggcc
     3001 aaatttatcc tgccaaagct gtttggtact ttcttgtaat ttttctgcgc ctgccaccac
     3061 atagcgtaga cgatagaagt catacgggtt ggcgaaacgc gcgtagtgac cgaggaaagt
     3121 cgaggtgccg aacaacacgg tgcaactgcg gtcatacacc aactccggca caatacggta
     3181 atgcagcggg cttggataaa gaaacacttc tgcacctgtg agcagtggcg taaacaggcc
     3241 taccgtcagc ccaaaggagt gaaacagcgg taacgccgac ataaagcgat cgttggtggt
     3301 gaagtcggca atcgttttaa tctgctcgac gttcgccaga atgcttttat ggctatggac
     3361 gacgcctttc ggatggcctt cagaaccgga ggtaaagagg atcagcgcct cttcttccgg
     3421 ctgctgtttt acctgtgcca gacgtggcat cagcagatgc gcgaagatcc atactttgtc
     3481 ggcagtggtg acatctgctt ttaaatcttc cagatagacc cagcgcacct gagtaagttg
     3541 ctccggcaga tgccagagtt tgcctttatc gagaaactgg cgggaagtga agatggtttt
     3601 gatttcagcc gccgtaatag cactggtcag cccttttacc ccggcagtgt agttcatcat
     3661 tgcgggaatg cggcgacggg cgatggcccc aaaaatcact gccgcactga tgcctgcatt
     3721 gggcagcatt aagccgatgc gttcgccttc aacactgtat ttttcaagga tgcgtccaac
     3781 aaacagcgtt ttcgtaagca atttgcgata ggagtctggg gtaaagttga cgtcttcgac
     3841 acatttcttc ccggctccga agcggtacat tgcactcagt aaagattcgt acagcgtttc
     3901 acgcgggcgc accgccattc gcgcttccat cattatttga tgcagcattt caccagcgat
     3961 tttgcgacgg tcacgggcac gcggcgcatc cggcatctcc acctgcgttg gtggcaaaat
     4021 atgcagagta atttgcggga acaagcgacg tttaaccaga cctttcaggc ggctgaagtg
     4081 cgtaagttcc gccccttcaa tacgcacagg aataaccgtt gcaccagact tcgccgcgac
     4141 aaaacccgcg ccatcgtaga ttttcatcag cgagcctgtg gtggtgatgc gtccttcagg
     4201 gaaaatcacc actggtcggc cctgttccac cagacgtacc agatgtttaa tcgccatagg
     4261 ttgcgtcggg tcgagaggaa caaagtcgat aaatgatttc agccaacgca tataccactg
     4321 ttggcttatt gaggtgtaaa cggcaaacac tggacgcaca ggtaaaaaca gtccaagcaa
     4381 aatgccatca ataaaagaga cgtgattagg cgtaattaga acgcgctcgc ccttcagtgc
     4441 ctgggtgtca cccgtaacgc gaacgcgata caaaacacgg cacaaatttc gaaaaaagct
     4501 aaaaagcatt ccaactccct ttgctctgat tcagtaaaag cgaatggagg gagattacac
     4561 gagataaaga acgcgagcga cagtaaatta ggtgcgaaaa aaaacctgcg catccgcgca
     4621 ggttggtgca agagacaggg tacgaagagc gtaccgaata atctcaccaa tcaatacctc
     4681 tgggatcttg attgtggtct gcacgacgac tcttcgccag cgagaaaacg caaaggaatg
     4741 aagggaaatg caacgaggtg tgtaaattgt cggttactgt tacagattga tgaccggcaa
     4801 aaaaaaacct gcgcatctgc gcaggctggt gtaattcatg tgctcaaccc gaagttgact
     4861 tcacctatca atacctctgg gatcaccact ttagcaacct gaagccaaac gccaccagca
     4921 gtcaatcgca acagcatttc gcaaagtgta accaaaggtt tgcaatctct ttttttgtct
     4981 tacttatttg cgtttggctc acattcccac gatgaaaaca cgccgcccct tgaaccaacg
     5041 ggcgttttcc gtaacactga aacaatgtaa gcgtttaccc actaaggtat tttcatggcg
     5101 accataaagg atgtagcccg actggcaggc gtttcagtcg ccaccgtttc ccgcgtcatt
     5161 aataattcac ccaaagccag cgaagcgtcc cggctggctg tgcatagtgc aatggagtct
     5221 cttagctatc acccgaacgc caacgcccgt gcgctggcgc agcagaccac tgaaacggtc
     5281 ggtctggtcg ttggtgatgt ttccgatccg tttttcggtg caatggtgaa agcggtcgaa
     5341 caggtggctt atcacaccgg taatttttta ttgattggca atggttacca caacgaacaa
     5401 aaagagcgtc aggccattga gcaactgatc cgccatcgct gtgctgcgtt ggtcgtccat
     5461 gccaaaatga tcccggatgc cgatttagcc tcattaatga aacaaatgcc cggtatggtg
     5521 ctgatcaacc gtatcctgcc tggcttcgaa aaccgttgta ttgctctgga cgatcgttac
     5581 ggtgcctggc tggctacgcg tcatttaatt caacaaggtc atacccgcat tggttatctg
     5641 tgctctaacc actctatttc tgacgccgaa gatcgtctgc aagggtatta cgatgccctt
     5701 gctgaaagtg gtattgcggc caatgaccgg ctggtgacat ttggtgaacc agacgaaagc
     5761 ggcggcgaac aggcaatgac cgagcttttg ggacgaggaa gaaatttcac tgcggtagcc
     5821 tgttataacg attcaatggc ggcgggtgcg atgggcgttc tcaatgataa tggtattgat
     5881 gtaccgggtg agatttcgtt aattggcttt gatgatgtgc tggtgtcacg ctatgtgcgt
     5941 ccgcgcctga ccaccgtgcg ttacccaatc gtgacaatgg cgacccaggc tgccgaactg
     6001 gctttggcgc tggcggataa tcgccctctc ccggaaatca ctaatgtctt tagtccgacg
     6061 ctggtacgtc gtcattcagt gtcaactccg tcgctggagg caagtcatca tgcaaccagc
     6121 gactaaccgc agttaaagca attccagcgc cagtaattct tcgatggtct ggcgacggcg
     6181 aatcaaccgc gcctgaccat tatcaaacag aacttctggt aacagcggac ggctgttgta
     6241 gttggatgac attgatgcgc catatgcccc tgtatcatgc agtaccagat aatcacccgc
     6301 cttcacttcc ggcaaggcgc gggtttcaac atttccccct tcctgctggg taaagacatc
     6361 gcccgattca cataatggcc cggcgacgac ggtttccacc gttggcgcgt gttccagaga
     6421 acgaccatca gctgccaggg cactgatatg gtggtaacta ccgtacattg ccgggcgcat
     6481 cagatcgttg aacccggcat caaccagcac aaagtggcgg ctacccattt gtttgacgct
     6541 ccgcacctga gtaattaaca cgccagactg cgctaccaga aagcgacccg gttcaatttc
     6601 cagtttcaca gggtggccca aatggcgagc gatttgctca cgcgcggcat tccacagacc
     6661 ataataatgt tcggtatcaa ccgcctcttc accctgttga taaggaatag aaagcccacc
     6721 gcccgcagaa atagcctgta aatcctgacc gaattcgatg acctgacgta ccatagcacc
     6781 acacacctgt tccagatggg cataatcaac gccagaacca atgtgcatgt gaatgccgac
     6841 cagctgcaga tgatgacgtt gtatcacgtc cagtgcggcg ggcagatcgg tgtaccagat
     6901 accgtgcttg ctgttttcgc caccggtatt ggttttttgg ctatgtccgt gaccaaaccc
     6961 cgggttaacg cgcagccata cccgatgccc tggcgaaacc tggcccagtt ggtcgagcat
     7021 atcaacagaa cccgcattca ccggaatttg caattcactg acgcgttcaa gcgtcgcctg
     7081 atcgataaca tctgccgtaa aaacaatatc atcggggtgc gtttgcggat tgtaacccgc
     7141 cgccaacgca cgctctattt cgcctaacga gacggaatcc actttcacgc cctgctcacg
     7201 cattaatcgc aaaatatgaa tattggaaca ggctttctgt gcaaagcgca ccacatcaaa
     7261 ctgtttcagc gctgcaatct gccgacgaat aatttgcgca tcgtagaccc acaccgggca
     7321 gccaaattca gcgggcaaac gcagcagatt ttcggcggtg agatcggtat cggtgctgaa
     7381 cagtgaatgt ggcataacaa actccagatg agtgcttttt tatgattacg ccacatcata
     7441 aaaagaataa aaaatatcga tttatgtcga gtctatgcaa aaatgatata gattaccgga
     7501 ttgcgagaga gcgctaatgg ccgccgttaa cttacgtcat attgaaattt ttcatgcggt
     7561 aatgaccgcc ggaagcctga ctgaggcagc acacctgcta cacacctcac agccaaccgt
     7621 cagccgcgaa ctggcgcgct ttgagaaggt gatcgggcta aaattgtttg agcgcgtacg
     7681 tggacgatta catcctaccg tgcaaggact gcgtctgttt gaagaagtgc aacgatcctg
     7741 gtacggactg gatcgcattg tcagcgccgc agaaagtctg cgcgagtttc gccagggaga
     7801 actgtctatt gcctgcctgc cggtcttttc gcaatctttt ttaccgcagc tcctgcaacc
     7861 ctttctggca cgttatcccg atgtcagctt aaatatcgtg ccccaggaat caccgctact
     7921 tgaagagtgg ctctcggccc agcgtcatga tttaggactc actgagacac tccatacgcc
     7981 tgcgggaaca gaacgtaccg aattactctc tttagatgaa gtgtgtgtgt tacctccggg
     8041 tcatccgctg gcggtaaaaa aggtattaac gccggatgat tttcagggtg agaactacat
     8101 cagcctttcc cgtactgaca gctatcgcca gttgctggat cagctattta ctgaacatca
     8161 ggttaaacga cgcatgatcg tagaaaccca cagcgccgcg tcagtctgcg caatggtacg
     8221 ggcgggggta ggtgtttcgg tggttaaccc actcaccgca ctggattatg cggcaagcgg
     8281 tttagtggta cggcggttca gtattgcggt tccgttcacc gtcagcctga tccgccccct
     8341 gcaccgcccg tcatcagcgc tggttcaggc gtttagtgag catttacaag cggggttacc
     8401 gaaactggtc acttctcttg acgcgatttt gtcgtcagct acgacagcat aaaagcgaca
     8461 gcatcctcgg catggatcgc cgcagtatca aacacaggca aaacactgcg ctcttctgga
     8521 accaataaac caatttctgt gcagccaaaa atgacaccct gcgccccctg ttctgcaagg
     8581 cgagcaatca cttgcgcata ataagcgcgt gacgcttcgg taaattgccc cagacacagt
     8641 tcttcaaaaa taatctgatt aatcttcgcc cgttcatccg cttccggaat aagacaattg
     8701 atggaaaatt attccgtcag ccgcccgcga taaaaatcct gttccatggt gtaacgcgta
     8761 cccagcagcg ccacacgagt cattccggcc ccggtaattg cacgtccggt ggcatccgcc
     8821 actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg ctgtaagcca
     8881 agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg ctggcactct
     8941 tctatttcat gaaaatcgac gctatgtagc agcacttgcg cagagtgaag cccaccaagc
     9001 cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc ccagctcatt
     9061 cctcccagca aaccaattgt tttcattcct ctctctcctg atgtgtgtta ccgcaacaag
     9121 gctgaataag gaacgagaca aacgcctcaa cggccaggtg cccaatctct attaacgaaa
     9181 aaagggccgg atgtacagca catccggccc gtgaaatcag acgccgatat ttctcaactt
     9241 ctcgcctgcc atcagtttgc gttcgatatg ttccagcgtg acatttttgg tttccggaat
     9301 gagccagaaa gtgatgccca caaacgcaat gttcagcgca gtgtagagcc agaacgtacc
     9361 ggcagcacca atgctgtcga gcagcgtcag gaaggtcgcg ccgataatca tattcgacac
     9421 ccagttcgtg gtggtcgaac aggtaatacc gaaatcgcgg catttcagcg gctgaatttc
     9481 agagcacagg atccacacca ctggcgcggc gctcatcgca taaccggcaa tacacatcat
     9541 cgtcatgcca acagagagcc aggacaagcc actggaagcc gtaccgttat caaactgcat
     9601 caggcaatag cccagcacca gagtgcctaa cgccatcacg ctgaaaccaa ttttcagagc
     9661 cggtttacgc cctgctttat ctaccgtaaa caccgcaata aaggtggcga acataaaggt
     9721 cagccctacg accagagtcg caatcatctg ttgttctgtg gtcgtaaagc ccgccatttt
     9781 gaagatacgc ggcgcgtagt acatgatgat gttcataccg gtaaactgct gcatcgcctg
     9841 caacaacata ccgagaaaca cagcacgacg gacgttacgg ttgatcttaa acagtgccca
     9901 accgccctgt tttaacttca ggctttcacg aatttcgttg agttcttctc gcgctttttc
     9961 cgacgtatcg cgcagcatac gcaacacttc ttccgcctca atatgacgcc ccttttccgc
    10021 cagccagcgc gggctatttg gcaggaagac caccagaata atcagcagga ctgctggtaa
    10081 agcaagaacc cccaacattg cgcgccagtt accgctataa ctgaacgctg tatcggataa
    10141 aaacgccagc acgatgccga gtgtgaccat caactggtac atactgatca tcttaccgcg
    10201 aacgttttca cttgccattt cagaaagata cagaggagcg gtgtaagacg cgatcccgac
    10261 agcaatgccc agcaccaccc gagcggcgat taacatctct acgctggtcg caaaagcgga
    10321 ccctatagaa ccgagtacaa acaggatggc ccccgccatc aggctgtatt tacgccccag
    10381 gcggaacgac agccaaccat taaacagcgc accaattgct gcgccgagca tcatgctact
    10441 aaccacccat tcctgcaaac gactggtcag cacaaagtga tcggtaatga acggcaacgc
    10501 tccggcgatt acgccgatat caagaccaaa taacaatcct gcgaccgcag cagctaccga
    10561 aacaaacata ttcatacgcc gcgtatcccg caaagaacgt ggcgttaaag cagattccgt
    10621 attgatagta accatttttt cctgccagca gagagtaaga catagtgaaa aaatacgtga
    10681 acaactcaca caggtgtcag gtcggaaaca gcataaatat ggattaaatt gctgcgacat
    10741 gtcgttatgt gatggatatt ccaattttca aattaagttg aattattgag attattatta
    10801 accacctaat tttacagcag ataaaattca taaagttcat taattgataa ttgatatgga
    10861 ttatttcata accatgatat gaattatgat gacctacagg tataaaaaac cctgccatga
    10921 ggcagggtca taaaagtaag aggaatgaat taacgagcca gccaaccgcc atctacggca
    10981 atggtataac cattcacata atctgaagcg ctggaggcaa ggaacactat cggccccatc
    11041 aggtcactcg gcagtcccca acgaccagct ggaatgcggt cgagaatttc cgcgctacgt
    11101 tgttcatctg cccgtagttg ttgagtattg ttggtcgcca tgtaacccgg agctatcgca
    11161 ttaacattaa tgttgtgttt agcccattcg ttcgccatca atcgcgtcac gcccatcacg
    11221 ccgctttttg atgcggtata agaaggcaca cggatcccgc cctggaagga gagcattgac
    11281 gtgatattga taatcttgcc gccattgcgt tgcgcgataa agtgtttcgc cgctgcctga
    11341 gacatgaaga atacgctctt gatattcagg ttcatgacat cgtcccagtc tttttcgctg
    11401 aactcgagag catcttcgcg gcgaatcaat ccggcgttat tcaccaggat atcaatatga
    11461 ccaaactcag ctaccgcgcg atccagcagt gctggaatgc catcaatctt tcgcagatcg
    11521 gcggtcaggc ttaaaaaacg acgccccagc gccgtgacct gcttgatggt ttcagtcggt
    11581 tcaacgatgt taatgccaac aatgtcacag cccgcttgcg ccagccccaa cgccatcccc
    11641 tggcccagtc cagtatcaca accagtgacg accgcaactt taccttcgag agaaaatgca
    11701 cttaaaatca taacaatacc ttattcgttt atgccgacaa ctagcgcaaa tctttaacgg
    11761 ccacatggtc catatcatca aagacctggt tttcaccaac catgccccag ataaaggtat
    11821 aagctttggt tccgacgccg gaatggatcg accagctggg ggagatcacc gcctgctcgt
    11881 tatgcatcac aatgtgacgc gtttcttgcg gctgccccat catgtggaaa acgcaggcgt
    11941 catcatccat attgaaatag aaataaactt ccatccggcg ctcgtgagtg tgacacggca
    12001 tggtgttcca caagttacct ggagccagct ccgtcagccc catactcaat tggcaggttt
    12061 ccagcacatc cgggacaaaa tatttgttaa ttgtgcgacg gttactggtg aggttatcgc
    12121 ctaacgtaac tggagatact tcgtccggtg tgactttttt ggtgggatac gtcgtatgcg
    12181 cgggtgcgca attgtaataa aactttgccg gagtggcggt atcaatactg gcaaagacaa
    12241 cttcttttgc acctttacca acatacaggg cgtcgcggtg accgatttca tagcattggc
    12301 catcgacagt aatcgtaccg gcaccgccaa tattgataac acctaactcg cgacgttcaa
    12361 ggaaatagct tacgccgagt tgtttaccaa cttccccgcc aacggaaacc gttttagtta
    12421 tcggcataat gccgccaaca ataattcggt caatgtggct gtaaaccatg gtgtactcat
    12481 cggcgacaaa taccttttca accaaaaatt cattgcgcag cccttgggta tccagcgttt
    12541 ttgcgtgcgc actgtggatg ctctgtctta cgtccacatc aacctccgat aatcagtgaa
    12601 gcgtgaaaat agaaaacgat agtgaaacac tgtttcgttt tctctataaa gggatgatat
    12661 gcccatgggc tagggttttc aattaaaaat aaaacaatgt ttcattttta agttaaggat
    12721 taaaaaaagt gcagtgtcga tcacgaatat gtcatgagaa tggaaaaatg agggagccca
    12781 gaaaatgtat gtcgacttgc cagccatcat tattcaacag attaatgacg gcagatattt
    12841 atcggtaagg aggaaatgca gagctactcg tcacgttcaa tggtcaatgc cacgccctgg
    12901 cccccgccga tacaaagcgt tgccagccct ttgcgggcat tacgtttcac catttcatga
    12961 accagagaaa ccaggattcg gcaaccggaa gcgcctatcg ggtgaccgag tgcgatcgcg
    13021 ccaccattga cattgacccg acgctcatcc cactcaagca tcttgccaac cgaaagcgcc
    13081 tgtgcagcaa acgcttcatt agcctcgata agatcgacat cagccaactg ccagcctaca
    13141 cgctccaggc aacggcgggt cgcatacacc ggcgcaattc ccatcaatgc cggatctaca
    13201 ccaacgctgg caaatgcgcg gatgcgggcc agcacgggta aattcaacgc tcgtgctttg
    13261 gcttcgctca tcatcattac cgcagctgcg ccatcgttta tggatgatgc attccccgcg
    13321 gtcacagaac cgagactatc aaatgaagga tttaaacggg ctaaaccttc tgcactggcg
    13381 tcagtgcgtg gctgttcatc ggtatcaaca accaacgtct gaccgttact ttggatcatt
    13441 accgggacga tctcatcttt aaatcgtccg gcgtcaatcg ccgctcgcgc tttttgttgc
    13501 gagctaagtg cgtaagcatc ctgcaactga cggctgatgc catattcgcg agccagattt
    13561 tcggcggtga caccaatatg ataatcattg aaggcatccc acaacccatc atgcaccaga
    13621 ctgtcaacca actggctatt gccaagctgt gcaccggtgc ggctatcagt cagaacatgt
    13681 ggtgcgcggc tcatgttttc ctggccaccg gcgatgacaa tatcagcctc gccacactgt
    13741 atcgcctgag tagccagatg cagtgcttta agtccggaac cgcaaacgtc attaatcgtg
    13801 attgcagaaa cgctattagg aagaccacct ttaatagccg attgccttgc cggattctgc
    13861 cctgcccctg cagtcaacac ctgaccaaga attacttcat ccaccgcata tgcaggaacg
    13921 ccggtacgtt ctattaacgc tttcacgacc agactaccaa gttccacggc ggaatgaccc
    13981 gctaacgcac cacgaaagca gccgataggt gtccgtaacg ccccgacaat cacaacgtct
    14041 ttcatcacaa cctcgcacca attaacagtg caatagtaaa tgattgttaa ttacagttat
    14101 cttaattgtt taaaaaaagt gatttttatc acaaaggaaa tatgcctgag cagtagtcag
    14161 agtcataact ggcacgtaag gtttgcaacc actaacccac caatagatgg gtagataagg
    14221 cgttaatctc ccatacttaa cctggtttat ggtaaattgc cctccatttt gtttaatttg
    14281 tagatgatac gttcagataa tgtctaatat ttggtctaaa gaagaaactc tgtggagttt
    14341 cgcgctctac ggcacagccg ttggtgcagg cacgctcttc cttcctattc agttaggttc
    14401 ggcaggggct gtggtcctgt ttattactgc tctggtcgcc tggcctttaa catattggcc
    14461 acataaagcc ttatgccagt tcatcctctc atcaaaaaca tcagcatgtg aagggataac
    14521 gggcgcggta acacactact atggcaagaa gattggtaat ctgattacca cgctgtactt
    14581 catcgccttt tttgtcgtcg tgttgatata tgcagtggca attaccaact cacttacgga
    14641 acagctggca aagcatatgg ttattgatct tcgcatccgt atgttggtga gtctgggtgt
    14701 tgtattaatt ctgaatctca tttttctgat gggacgtcat gccactattc gggtaatggg
    14761 atttttggta ttcccattga ttgcctattt cttatttctt tccatttacc ttgtcggtag
    14821 ttggcaacct gatctattaa cgacccaggt agagttcaat cagaataccc ttcaccagat
    14881 atggatatcg attcccgtga tggttttcgc ctttagccat acgcccatta tttctacgtt
    14941 tgccatagac agacgtgaaa aatatggcga acacgctatg gataaatgca aaaaaattat
    15001 gaaagtcgct tatctcatca tctgcataag tgtactgttc tttgtcttta gctgcctgct
    15061 ttctattcca ccttcgtata ttgaagcggc taaagaagaa ggggtcacca ttttatcggc
    15121 gctttctatg ctgccgaacg ccccagcatg gttgtcaatt tccgggatta ttgtcgcagt
    15181 agttgcgatg tcgaaatcat tcctgggtac gtactttggc gttattgaag gtgccacgga
    15241 ggtcgtcaaa acaacactac agcaggttgg tgtaaagaaa agtcgtgcat ttaaccgcgc
    15301 actatcaatt atgttggtat cgctgattac cttcattgtt tgttgcatta acccgaacgc
    15361 gatttcgatg atttatgcga tcagcggccc gctcattgcc atgatacttt tcatcatgcc
    15421 tacgttgtca acgtatctga tcccggcgct taaaccctgg cgctccatcg gaaatctgat
    15481 tacgctgatc gtgggtatcc tgtgcgtatc ggtaatgttc tttagctaat atccgttgtg
    15541 gatggttcaa aattgctaca attttgaacc attccatttt tcataatatt catttgttat
    15601 tattccgccc tatcacttct ctcgtcatat cctagaatta tcagttagtt aaacacacaa
    15661 agagatctga acatcttaaa taagactatt taagatgcat aacttagatt cgcaagatat
    15721 cttctggtca ttcaaaaaca atttgcgata tagatattct cataccatga gatagttatc
    15781 tggaccacgt tcgaccatgc aagattaaat cagtgaaaca taatcatatt taataacgag
    15841 aaatgcattt ttaaatgtct taatcagaat tatagcaaat acagattaat cgcaattttt
    15901 cccagcaaaa ttacaaaatc aagatgtatt gacatcatca atcaccaatg aaattatgca
    15961 attatatacg gatagggagg ttcttaacat ggggctttgt agtcgttata aaagtcttac
    16021 atgcaatagt tgctcaatgc attgccaaat aatgccagaa gagtcaccgc gtttgcagta
    16081 ttgcgcaaac tcgtgttttt gtatgtggcc cgaagaaagt tcatatttta atcgtggtgt
    16141 ggtggaaggt atcttaacaa aaaaccacaa tgcgagatta agcggatata tttttgtgga
    16201 tttttcagta agttttttac gtctattcct tgaaaaagac tggattgact atcttgcaag
    16261 tactgacatg gggatagttt tagtcagcga ccgcaatatg caatcgttag ctaactactg
    16321 gcgaaaacac aacaccgcaa tatctgctgt catatataat gatgatggtc ttgatgttgc
    16381 caatgaaaag atcagacaac tatttattgg tcgttattta tcatttacca gaggtaacac
    16441 attaactcag atggaattca ccataatggg gtatatggtt tctggttata atccatatca
    16501 gattgctgaa gtgctggata tggatatccg tagcatctac gcgtacaagc aacgaatcga
    16561 aaagagaatg ggtggtaaaa taaacgaatt atttattcgt tcacattcgg tccaacattg
    16621 atactcaacg accagccaga atcatactct gttcatacgg gcatctattg aaatgcccgt
    16681 attttataaa taagcagtaa ccccaagagt aaacaacata gtatttaaaa ccatcacacg
    16741 aaaaattcag aagcattacg aaatatgaat ttacaaacaa tagtggcata aatgttaacc
    16801 atgttaattt acgtaaagtt ttacgttgca acattaaagc ctcattt
//
LOCUS       DAEEKQ010000081        16480 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.081, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000081 DAEEKQ010000000
VERSION     DAEEKQ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16480
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.081"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(49..201)
                     /locus_tag="KLK57_002516"
     CDS             complement(49..201)
                     /locus_tag="KLK57_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBE0892858.1"
                     /translation="MLTKYALVAIIVLCFTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(466..1200)
                     /gene="cysH"
                     /locus_tag="KLK57_002517"
     CDS             complement(466..1200)
                     /gene="cysH"
                     /locus_tag="KLK57_002517"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HBE0892859.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIRPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1275..2987)
                     /gene="cysI"
                     /locus_tag="KLK57_002518"
     CDS             complement(1275..2987)
                     /gene="cysI"
                     /locus_tag="KLK57_002518"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HBE0892860.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIITGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(2987..4786)
                     /gene="cysJ"
                     /locus_tag="KLK57_002519"
     CDS             complement(2987..4786)
                     /gene="cysJ"
                     /locus_tag="KLK57_002519"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HBE0892861.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLKGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     PQTPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5105..5467
                     /gene="queD"
                     /locus_tag="KLK57_002520"
     CDS             5105..5467
                     /gene="queD"
                     /locus_tag="KLK57_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HBE0892862.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5545..6816
                     /locus_tag="KLK57_002521"
     CDS             5545..6816
                     /locus_tag="KLK57_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE0892863.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLEP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6807..7067
                     /locus_tag="KLK57_002522"
     CDS             6807..7067
                     /locus_tag="KLK57_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HBE0892864.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7084..7659
                     /locus_tag="KLK57_002523"
     CDS             7084..7659
                     /locus_tag="KLK57_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HBE0892865.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGGMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7807..8667)
                     /locus_tag="KLK57_002524"
     CDS             complement(7807..8667)
                     /locus_tag="KLK57_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HBE0892866.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8664..9443)
                     /locus_tag="KLK57_002525"
     CDS             complement(8664..9443)
                     /locus_tag="KLK57_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HBE0892867.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9421..10830)
                     /locus_tag="KLK57_002526"
     CDS             complement(9421..10830)
                     /locus_tag="KLK57_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0892868.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVDFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10852..12306)
                     /locus_tag="KLK57_002527"
     CDS             complement(10852..12306)
                     /locus_tag="KLK57_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBE0892869.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSRPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTDFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12376..13192)
                     /locus_tag="KLK57_002528"
                     /pseudo
     CDS             complement(12376..13192)
                     /locus_tag="KLK57_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13510..14787
                     /locus_tag="KLK57_002529"
     CDS             13510..14787
                     /locus_tag="KLK57_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0892870.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIFQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFGVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSIIGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALGMVIVFTFLLFQKIRTADSAPAIASSK"
     gene            14814..16299
                     /locus_tag="KLK57_002530"
                     /pseudo
     CDS             14814..16299
                     /locus_tag="KLK57_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311659.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
BASE COUNT         3901 a         4351 c         4155 g         4073 t
ORIGIN      
        1 accaaaccat cacaattcag tacgcggggt tttacccccg cgtggtggct acttcttcga
       61 ttcgtaagcg agaactgcct taaactccat accacgttct ctgatactca actcacacaa
      121 cgagtcgcct accatcagcg tgaatcccag tactgtaaaa cacagcacta tgattgccac
      181 aagggcatat tttgtcagca tgtcttgcct ccctgcaaag aagagactaa cattcgaatt
      241 gtttgggttc gaacgctggc ctcaggttga tagaaatatc gcctggggct tttgtccatc
      301 tggaacctcg cgaatgctta acgccagaca gcctcaagca cccgacgcca ttctatacct
      361 gataattctt ccagcggcgt tcacgtttaa aatcaggaat actgctcgca aaataaaaac
      421 gcccccatca tttctgacag aggcgtttaa tttatccggc aatatttacc cttcgtgcaa
      481 tccacattcc ctttttaagc caaagaaacg tgtttcttct tccgacatgc caggttccca
      541 tttacgggtt gtatgggtat caccgaccga taaatatcct tcatcccata atgggtgata
      601 tttcaggcca tgtttttgca ggtactgata aatagttcgg ttatcccagt cgataatcgg
      661 cagcacttta aatacgccac gctgaattgc cagcaccggt aaattggcgc gactgccgga
      721 ttgttcacgg cgcaggccag caaaccaggt ttgcgcgtta agttctttca gagcccggtt
      781 catcggttcg actttgttga tgtcattgta cttttcaatg ccttcaacgc cctgctccca
      841 cagttttccg tagcgtgctt cctgccaggc tgcgctttcg gtagcacggt acactttcag
      901 gttgagcttg agtttgtccg ttaactcgtc aataaagcgg taggtttccg ggaacaagta
      961 acccgtatcg gtgaggatca ccggaatatc cgggcgaatt tgattcacca gatgcaggct
     1021 caccgccgcc tgaatgccaa agctggaaga aagtacatat tcaccgggca gattatccag
     1081 cgcccaggct acgcggcctt cagcgtccag tttttccagt tcggcgttag tttccgccag
     1141 cgccagaata cgatctacct tcggcagttc gttcagggcg tttagatcga gtttggacat
     1201 agattcctca ctgtttgcct tgcctgatgc gacgctcacg cgttcttatc aggcctacaa
     1261 gaccgggctg atggttaatc ccacaaatcg cgcgccggat cgagcaccgg gcgaatgatg
     1321 cccgcacgca ccgtaaagtc gccgaagcct tcacccgctt cgcgctcttt cgcccagcgc
     1381 cctatcagtt catcaagcga cgccaggatt tccggctcgg tgatgttttc tttatacatc
     1441 cgtgggatac gtgtcccaat gcggttgccg ccaagatgca ggttgtagcg acccggcgct
     1501 ttacccacca ggcccacttc cgccagcatc gcgcgaccac aaccgttcgg gcagcctgtt
     1561 acacgcatca cgatatgctc atcgctgaca ccatgtttcg ccattaaatt atcgatgttg
     1621 tcgataaaag acggcaggaa acgctctgct tccgccatcg ccagcgggca agtcgggaat
     1681 gacacgcagg ccatcgagtt ttcacgctgc ggcgtgacgg cattcattaa cccgctctct
     1741 ttggcgatct tctcgatctt cgctttctcg ctttccggta caccggtgat gatcagattc
     1801 tggttcgccg taatgcggaa atcgcctttg tggatcttcg cgatctccag caggccggtt
     1861 ttcagcggac gccccggata atcaaggatg cgaccatttt cgataaacag cgtcaggtgc
     1921 cagttatcat caatgccctt aacccagcca atacgatcgc ctcgtccggt gaactcatat
     1981 ggacggatcg gttcaaattt gatccccgcg cgacgctcca cttccgcttt aaacgtctca
     2041 acccccacgc gctccagcgt gtatttggtt ttggcatttt tacgatcggt tcggttaccc
     2101 cagtcacgct gagttgtcac gacggcttcg gccaccgcca gcgtatgctc cagcggcaga
     2161 tagccaaact cgctcgccgt gcgggcgtag gttttcttgt tgccgtgttc gatagaaagc
     2221 ccaccgccca ccagcaggtt gaagcctacc agcttgccgt tttcggcgat tgccacgaag
     2281 ttcatgtcgt tggcatgcag atcgatatcg ttctgcggcg ggatcactac cgtggttttg
     2341 aacttacgcg gcaggtaggt ctgaccaagg atcggttctt catcagtagt ggcgactttt
     2401 tcctggtcga gccagatctc cgcatacgcg cgggtacgag gcaacagatg ctcagaaatc
     2461 ttcttcgccc actcgtacgc ttccgcgtgc agctgcgact cgtaagggtt cgaggtgcag
     2521 agtacgttac ggttcatgtc gttagctgtc gccagcgcat caagaccgac cgagtgcagc
     2581 atctggtgca ccggtttgac gttctttttc agaatgccgt ggaactgaaa cgtctggcgg
     2641 ttggtcaggc gaatgctgcc atagatggtg ttttcaccgg caaatttgtc gatcgcctgc
     2701 cactgtttag tggtaatcac cccacccggc agacgacagc gaagcagcat cgcgtggcgc
     2761 ggctccagct tctgttcagc acgttcggca cggatgtcgc ggtcatcctg ctgatacatg
     2821 ccgtggaagc ggatcagcag gaagttgtcg cccttaaagc cgccggtcag accgtcgttt
     2881 aaatcttccg caatggtgcc gcgcaggtag ttgctttcaa gcttcatgcg ctcggcgtct
     2941 gtcagttttc cttcgaccac taaaggccct ggatgttttt cgctcattag tagacatctc
     3001 gctgataacg gcgctctacg cgcagctcac ttaaaaattc atccgccgct tcggtgtcca
     3061 tgccaccaaa ttcggcaatc acttccagaa gtgcctgctc aacgtctttc gccatgcgat
     3121 tagcgtcgcc gcagacataa atgtgggcac cgtcattgat ccagcgccac agctccgcgc
     3181 cctgttcgcg cagtttgtct tgtacataaa ctttttcttt ttggtcgcgc gaccaggcaa
     3241 gatcgatacg cgtcagcacg ccctctttga cgtagcgctg ccactccacc tggtagagga
     3301 aatcttccgt aaagtgcggg ttgccaaaga acagccagtt tttacccggc gcttcgtcgg
     3361 cggcgcgttg ctgcataaag gcacggaacg gcgcgatgcc ggtaccgggg ccaatcataa
     3421 tcactggcgt ttgcgggtta gcgggcaggc ggaaattgtc gttatgttcg ataaatacgc
     3481 ggacttcgcc ctcttcttcc acacgatccg cgaggaagct ggaggcacca ccggcacggg
     3541 cgcggccttc cacgtcgtaa cgcaccacac caacggtgac gtgtacttcg ttctcgactt
     3601 ccgcctgcga ggaggcgatg gaatacaggc gcggggtcag cgggcgcagc aggttaatca
     3661 gcgcttcggc atccagttgc gccggagaga aacgcaccat gtcgacaatc ggcgtggtcg
     3721 cggcgtaatg ctgtaacttc gctttatcgc ccaccagcgg cagcagcgtt tcgctgcggg
     3781 taagcgtggc atagttctca acaatgttgg cggtgttgac ggtcagttcg aagtgccact
     3841 gtagcgcttc gttcagaggc aacgttttgc cctcgacggt gacaggttca tcgcctttca
     3901 gccacagcag ttcgacaagt tctttcacca gtgccgggtc gttctgatac cagacaccca
     3961 gcgcgtcacc cggctggtaa cgcaggcctg aatcgcctaa gtcaatttcg atatgacgaa
     4021 cgtctttttc agagttacgc ccggtaattt tctggttaac cgaaaggctc gccaccagcg
     4081 gcgcgtcttt gctgtacggg ctggtgtgga tttcatttac cgcgccagta gcgacggatt
     4141 gcgaaggtgc cgcgacaggc gcacgcgatt taagcgcatc aaccacgcgg gcgcgccact
     4201 cgctggcagc agcctggtat tcaacatcgg catcgacacg gtcgagcagg cgttcaccac
     4261 ccagttccgc cagcttgctg tcgaaatctt tcccggactg gcagaaaaat tcataagagc
     4321 tatcgccgag gctaaacacg gcaaacgcgg tgttttccag ctttggcgct tttttggaga
     4381 acaggaactt atgcagcgcg acggcttctt ccggcggttc cccttcccct tgcgttgacg
     4441 tcactacgat gagcagtttt tcgctggcga tttgtttgaa tttatagtcg cccgcgttca
     4501 ccagcttaac gttcagtttt gctgctaata aatcatcgcg taatgcttca gcaacccggc
     4561 gcgcattgcc ggtttgcgag gcggagataa tagttatacc cggcatttct gcggctggcg
     4621 ctggcgtcgc tgcaagcgca gcaggctgct gattgagtac gccccagaaa tagccagaaa
     4681 cccaggcaag ctgggtaggc gttaaatcgg tcgtggccgc ctgaaggcgt gccagttgct
     4741 ccgggttcaa cggaagcaac gcggaaggtg ggacctgtgt cgtcatgcgt cgttatgttc
     4801 cagtaagcaa agctgtttct gcgccctgtc agcgcccata aaacagaaga gaagataagg
     4861 ttaacggggt gaacggtgtg gattaaagac gggatagcga taactaataa ccaaatcgac
     4921 taacctgttt tagcaatagt ctttaacaac aaaatagatt aaccaaccca atgaaaaaca
     4981 aatgaattta gccaatcatt aagataaatc agcgattttg cgcaacaaag tcgttttaga
     5041 taatgcgaaa aaacagcctt tccggtactc tacggcggtt ttatcgtcct gtagagaaat
     5101 tatgatgtcc accacgttat ttaaagattt caccttcgaa gccgctcacc gcttaccaca
     5161 cgtcccggaa gggcataaat gtggtcgcct gcacgggcat tcctttatgg tgcgactgga
     5221 aattaccggg gaagtcgatc cgcatacggg ctggattatc gatttcgctg aactgaaagc
     5281 ggcgtttaag ccaacttatg agcgcctcga tcaccattat ctcaatgata ttccaggtct
     5341 ggaaaaccca accagcgaag ttttagcaaa atggatttgg gatcaggtta aacccgttgt
     5401 gccgctgtta agtgcggtga tggtaaaaga aacctgcacc gcagggtgta tctatcgcgg
     5461 cgaatgataa gagtgtgtcg gcggtcaatt tcccttaagt aacgctatgt tagggtgttg
     5521 tgttctggat atctggggca tgacatggaa gacgactgcg acattattat tattggtgcc
     5581 ggtattgcag gcaccgcttg cgcgttacgc tgcgcgcgag cgggtttatc cgttttgtta
     5641 ctggaacgcg ctgaaatccc cggcagcaaa aatctttccg gcgggcggtt atatacccat
     5701 gcactcgcgg aactcctccc gcaatttcat ctgaccgcgc ctcttgaacg acgcatcact
     5761 cacgaaagcc tttccctgtt aacgcccgat ggcgtaacga cgttttccag cttacagccc
     5821 ggcggtgaat cctggagtgt attacgtgca cggttcgatc cgtggctggt tgccgaagcc
     5881 gaaaaagaag gtgtcgaatg catccccgga gcgacggtgg atgcactgta tgaagaaaac
     5941 ggcagagtct gtggcgttat ttgtggtgac gatattctcc gcgcccgtta tgtggtgctg
     6001 gcagaaggtg ccaacagcgt cctggctgaa cgtcacgggt tagtgactcg tcctgctggc
     6061 gaagcgatgg cgttggggat caaagaagtg ctgtcgctgg aaacatccgc tattgaagaa
     6121 cgttttcatc tggagaataa cgaaggcgca gcgttgctgt tcagcggcgg aatctgtgat
     6181 gacttacccg gcggcgcatt tctttatact aatcaacaaa cgctctcgtt agggattgtt
     6241 tgcccgctct cttcccttac gcaaagtcgt gttccggcaa gcgagctgct gactcgcttt
     6301 aaagcgcatc cggcagtgcg cccgcttatc aaaaacacgg aatcactgga gtatggtgcg
     6361 catctggagc cagaaggtgg cttgcacagt atgccggtgc aatacgccgg taacggctgg
     6421 ctgctggtgg gcgatgcgtt gcgcagttgc gtcaataccg gaatttccgt gcgcggcatg
     6481 gatatggcgc tgactggcgc acaggcggcg gcacaaacgc taataagcgc ctgccagcac
     6541 cgcgagccgc aaaatctgtt tgcgctttat catcacaacg tagagcgcag cctgctatgg
     6601 gatgttctac aacgttatca gcatgttccg gcgcttttgc aacgccctgg ctggtatcgg
     6661 gcgtggcctg cgttaatgca ggatatttcc cgcgatttat gggatcaggg tgataaacct
     6721 gttccaccgc tgcgccagtt attctggcgt catttacgtc gtcatggcct gtggcatctg
     6781 gcgggcgatg ttatcaggag tctgcgatgt ctgtagccca taatctctgg cgcgttgctg
     6841 atgcgccgca cattgttccg gctgactccg ttgagcgtca gatggtacaa cggttgatta
     6901 acgcctgtcc ggcaggtctt ttttcgctca caccggaagg tgacttacgc gttgattatc
     6961 acggctgcct ggagtgtggc acctgccgtt tgctgtgcga cgaatccaca ctacaacagt
     7021 ggcgctatcc gccttccgga ttcggcatca cctaccgctt tggataaata ataaggataa
     7081 tttatgcccc tcttacacct actccgccag aatccggtga ttgctgccgt taaagataat
     7141 gccagcctgc aactggctat cgattctgaa tgccagttta tttccgtgtt gtacggcaat
     7201 atctgcacca tcagtaatat cgttaaaaag attaagaacg ccgggaaata tgcttttatt
     7261 catgttgatt tgctggaagg cgcgtcaaat aaagaagtcg ttattcagtt tttgaaactg
     7321 gtgaccgagg cggacggcat tatcagtacc aaagcctcaa tgttgaaagc ggcaagagcg
     7381 gaaggttttt tctgtattca tcgcctgttt attgttgatt caatttcgtt tcacaacatt
     7441 gataagcaag ttgcgcaatc gaatccggat tgtattgaga tcctgccagg cggtatgccc
     7501 aaagtgctgg gctgggtgac agagaaaatc cgccaaccgc tgattgccgg tgggctggtg
     7561 tgcgatgaag aagatgcgcg taatgcgatt aacgcgggtg tcgtggcgct ttccaccacg
     7621 aataccgggg tctggacgtt agcgaaaaaa ttactttgac gggataatcg taaccaattg
     7681 aatttggttt gatttgtagg ccgcacgcca catccgacat tcagcgcctg atgcgacgct
     7741 tgacgcgtct tatcaggcct acaagtcccc aggccagaac cgtaggcagg atcatccggc
     7801 aacacgttat tggcaatctg cgtgaatgtt tgtcaccagt gcctccagca ccactttcca
     7861 gtcatcaacc acgccaacat ctgcctgtga aaataccgca gcgctggcgt catgattaat
     7921 cgccaccaca aacttgctat tgcgtacgcc cgccattaac gctgctgcgc cagaagcgcc
     7981 aacaacaata cacacctcag gtgccagcag atgtccggaa ataccaatca ctttttcagc
     8041 atcaacgcca ccgttcatca ctcgcgcccg actgtagccc acttcggctc ccagcttttc
     8101 tgccagcata gcgatttcct ggttatctgc ctctccgccc tgccccacca ccagcacccg
     8161 tctggcctca gctaatgggt cgcgagtaac atttttcaga tcctctgtgc taaccagcca
     8221 gtccggaagc gcacccggga caatgttcag ttgttgcatt ccagagggca acgtggcgtt
     8281 tttcgctgct cccgcctgac gcgccaggga aaggcatagc gggcgttttt ctgtttgcag
     8341 cgtcgccgtt agcgcatttc cccagtggga tttgcgcacg cttacggttg gaatatctaa
     8401 cgaagtcacc tggcagatgc ttgcaccatg taaacgccag gccagccggg tcgacagttc
     8461 atcgccaaac gttcccggcg gaaagagaac aacgtccgca ggcgttcgct gccactgttc
     8521 caccaacgca tccagcacct gttccgccac tacaggctga ggttcaatct gccagtgcgc
     8581 cagggtacaa ccgctgaaat cctgcgccgc cagccagctt gctattgcgg cattctcctg
     8641 atttattgtc acaattgcga tattcatggc tgcatccttt ggcgtaaata gtcctgccat
     8701 agcttttgag ctttttctgc taccgtttgc ccatcaatca gcgtcgctcc gcgtcgttgc
     8761 tctgctcttg ccagttgcag acactgcatc gctggcattt cggcagcaac agttttacgg
     8821 ataatttctg ctttccccgc agccattcgc tggcgcattc ccggtactgg caaggccact
     8881 tcaccgcact gacgcacggc aatcaccgca ggcaagcgaa cccggcaaca acgcaaccca
     8941 tgttcagtac gctgttcgag ggtgataaac agcgcgtcga gagtgaaacg ctccacctgg
     9001 gtgaagcagg gccagcccag catttccgcc agcaaaaatg gcgtttgccc attctgccct
     9061 tcgctgcttt ggcagccggt aattatcaga tccagcggat tctgatgctg ccattcggca
     9121 atatggcgag cgacaaattc cggcgcaaag cgcagatctg ccgccgtctc cagcaataca
     9181 gcttcctcaa acccaagagc catgagatag cgcagccagt gcaatgcccg ttcatccccc
     9241 atgctcaacg cggttaaaga catcggcgta ccgttttttc tctgcgccaa cagcagcgcg
     9301 gcagcggcct gttcatcagc accgagtaaa cttcgcagta gcgaaatatc cggtccgctt
     9361 ttaccctgag ccgccgcctg ccactctttt tccgccagca ttccggcatc cggttcggct
     9421 ttaaacgcta acagaatgtt cattcgcacc tcctacattt cccgccgcca ccagcgggag
     9481 ggctttagtt tctggtgccc atagccaggt cacaacaaaa tcaaccaaca acactgtcgc
     9541 caggagcaat aaggtgactt gcattcccca ctgcgccagc acccacggca gcaggccagt
     9601 acttaccgcc gcgccaagtc gactcatggc ggtggcaaaa ccgacgccca gcgagcgaat
     9661 gtcagtagga aaactttccg caggcaaaat gcccaccaga ttactgactg ccgaaatggt
     9721 ggtgctgaag agaacaaaaa gcagcagcgt taatgaactg ccggaaggca aacaggccat
     9781 cactaccagc gttgccgcca gcagcaaaaa acttcccagc aaaaatttgc gatgtgccag
     9841 caggtgcgtc agaactaatc ccagcagcgc gcccacaatt aacaacgcat taagcatcag
     9901 gctggcagtc agcgcatctt ccagaccaat agtctgggcg atagttggca gccaggtata
     9961 aatcacaaac catgggatta cgaggcagac aaagaagacg ctgttaaacg ccgtgcgccg
    10021 ccagtaacgc gaagagaaca aggttttgat gtgtttatgg gtcgccgtta ccacttcatc
    10081 gcccagtaaa acatggggac caaaatagcg atgcacgata gcgtgagctt ctgcaaaacg
    10141 cccctggcgc agtagccagc gtggtgattc tggcgttccc cagcgtaata acgtaatcaa
    10201 caacgcgggc agagctgccg atgccagtag ccagcgccag gcctccgggt tttcggaaat
    10261 aaagtgatgt ccggcaatac ttgccagcac atagcctacg gtccacacca cgctgaatgc
    10321 gcccagcaaa ataccgcgat ggcggcgcgg ggaaaattca gccagcaagg tgtgacctac
    10381 tgaataatcg cctcccagac caatgccaat caaaatgcgc agtccaataa gatgctctgg
    10441 cgtggtggca aaaaattgta aaaacgaagc aagcgtaatc agcaaaaagc tgaaggtgaa
    10501 gattttttgc cgaccaatat ggtcggagat ccacccaaga accaggctgc caaggaacaa
    10561 accaaggagc gccgagccgc cgatcattcc cgccataaac ggcgtcagtt gcatggcggg
    10621 cgtaagctga ataatggcgt aaccaatgac gccgagaaca taaccgtcgg tcaggtgtgc
    10681 gccgaaagtg agcgcagcaa tgcggcagtg aaaacggtta agcggtaaat catccattcg
    10741 caccggtgaa gtgttcatct ttaccccttc attgtccggt aataagaacg gcttctcgcc
    10801 tgagaagccg aaaaggcaac gccccgtcat cgcggaacgc tgcctgacac attatttttc
    10861 aatcggatag atagtacccg tattcataat gccattagga tcgaactgct ttttcagccc
    10921 ttccagcaac gcccacgcgc tgccgtgttc cagtttgctc cagtgaacgc gatgtttacc
    10981 gataccgtgg tggtgcacca tcgaaccgcc gaggcgaatg gtttcttcac agatgatctt
    11041 gttgagcgga ttgtggtact tgtcgatttc ctcttccggc ttacagtcaa cgacgttgta
    11101 atcgtagacg aagtacatgt tggtgccgtt ctgatagcta tgagaggaat gaccgcccag
    11161 catggtgatg tcgtcggcgt gcgggaactc agtacgaata cggttaataa cgctttcgta
    11221 gatttcgtgg atgcagctcc agcagccgga cacttcagtg gtaaagccca tgttgccggt
    11281 tttgaggatc tgcacacgtt cggcagccac tttatccggt ccccagttca ggttgttaaa
    11341 ccaggtttcg atcagcttgc tgtccacgcg ctggcattgc gggtagcggg caacgatttc
    11401 cgcaatccct tcgcccgtcg ccttcgcaat gcgagggtta ccttcagcca taaagatcag
    11461 cacgcatttt ccgtctgcaa aatgggtgaa gtgttgggtg ccatcttcag cgtcatacaa
    11521 acgagcgatc gacggacgat acccttccac catgacttca cgcaggatgt tgaagtcggt
    11581 tttcatgtct tccaggatat agccgtagaa gaggttgttt tccggggtga atttaaagat
    11641 tttcactgtt acttcagtga tatagcaaag tgcaccttcg ttaccgatga tgatgtggcg
    11701 aatgtccggg ccagccgcgc ggcgtggcac gtttttaatg cgtgtgacgg tgccatcagc
    11761 caatactgct tccagaccaa cgaccatatc ttcgattgcg ccgtagagtg tggagaactg
    11821 cccgatactg cgggttgcta ccaagccgcc catctgcgcc agcggccttg actgcggaga
    11881 atgccccgtg gtgtaacctt tttcacgcaa cgcattttcc agcacctcca gcggaacgcc
    11941 acattgcgcc gtcgcctgca tattctcaat atcaatatta atgatttgat tcatggcgga
    12001 gccgtcaagc accaccgagt tttccacaac ggtttccagc ccaccttcag tggcggaagc
    12061 gccggtacgc ggcacaccgt taattttgtg cgcattcata aaattcagca cacgggatac
    12121 ttgctctgtg gaaccgagtt ttacgaccgc tgccggaatc ggcaaagtat aaatgccatg
    12181 aatatccgga aatttacgaa aacggtcaat actgttcttc tttaatactg tttcatcggt
    12241 aattacgcga tctgcaccaa caatttcctt tagttggtcg acaatcgctg cgcgagataa
    12301 agacatagta attccttcct gataataaaa atggaataat cattaataag agaaaacaga
    12361 aaccaccgtt atggattagc gcactaaata accaccattg accactaata aatgcccatt
    12421 gacataattt gaagccggac ttgcgaggaa tacagctgcg cccattaaat cctgagtatc
    12481 gccccaacgg tttgccggaa tatgatcaag aacgcgctga ttggtttctg gattactgcg
    12541 tgtcgccagc gtaatatctg ttgcataata gccaggagca ataccattta cctgaatatt
    12601 atattgacct agttcatcac aataagcttt ggtgaatccg gcaagagcat gtttagtggc
    12661 agaatatgca ggtgaccatt gtccacctaa gtaagagaac aatgaacaga tattaataat
    12721 tttgccgctt ttctgcggga tcataatttt tgcagcttca tagcttaact cgaatgcggc
    12781 ggtcaggttc acatcaatca tcggatccca gtcggcacga ccgaagtcca gcaccttatt
    12841 cagcttacaa ataccggcat tgtttaccag aatatcaact gtaccgaaac gctcacagca
    12901 agcagcgata atcttctgcg gcgcgccttc tgcggtgata tccacctgca tgaagtccac
    12961 ctcaacaccc tgtttttcaa tcatttcctt tgtttcgccg ttatctttca atcatttcct
    13021 ttgtttcgcc gttatctttg acgaaactag gaataaagat atttgcgcca gctttggcca
    13081 acgccatggc aaatgcctgg cctaaaccgc tattcccacc ggtaacaatt gcggttttac
    13141 ctttcagtga gaaaaaatcc attgagaacg cattgagaga ttcgattgac atagttagct
    13201 ccaatttccg taaggcaaaa aaaagaaagg taacttcccc cgcgagaggg aagttacctt
    13261 tctcattatc tctggtaact ggagataatt tttagcataa cggcataact gctaatgtga
    13321 tcgtaatcac agtgtgatat tcgtattgtc aaatagtttt aaaaagcaga agcgtgatta
    13381 ctctcacaca acacctctgc tttccatgtt atttcttatt gcaattacta gagacctgag
    13441 aaaagtaatc cacctgaaag ggcggcttac ttttctcttt tttttgtcta ttacaaatgg
    13501 gaatacgata tgcaacacaa ctcatatcgc cgttggataa ccctcgcgat aattagtttt
    13561 agcggcggcg ttagttttga cctggcttat ttacgttata tttttcaaat tcccatggcg
    13621 aaatttatgg gattcagcaa taccgagata ggtttaataa tgagtacctt tggtattgcg
    13681 gctattattc tttatgcccc cagcggcgtt attgccgata aattttcaca ccgcaaaatg
    13741 attacttccg cgatgatcat taccggatta ctgggtctgt taatggcaac gtatccaccg
    13801 ctgtgggtaa tgctctgtat tcaggtcgcc tttgcgataa cgacgatttt aatgctgtgg
    13861 tcggtgtcga ttaaagccgc ctcgttgctg ggcgatcata gtgagcaagg gaaaattatg
    13921 ggctggatgg aagggctgcg cggcgtcggt gtaatgtcgc tggcggtgtt taccatgtgg
    13981 gtcttttctc gctttgcacc ggatgacagc gccagcctga aaacagtcat tatcatctac
    14041 agtgtggttt acatcttgtt ggggattctg tgctggtttt ttgttagcga taacaacaac
    14101 ctgcgcagtg ccaataacga agaaaaacag tcattccagc ttagcgacat ccttgccgtt
    14161 ttgcgtatca gcaccacctg gtattgcagc atggtgattt ttggtgtctt cacaatctac
    14221 gccattctga gttactccac caactatctg accgaaatgt acggcatgtc gctggtggca
    14281 gcgagctaca tggggattgt gatcaacaaa atcttccgcg cgctgtgcgg cccacttgga
    14341 ggcattatca ccacctacag taaagtgaaa tcccctaccc gcgtgattca aatcctttcc
    14401 atcatcggtt tgctggcgtt gacagccctg ctcgtcacga actctaaccc ccaatcggtc
    14461 gcgatgggga ttggcctgat tttactgctg ggattcacct gttacgcctc acgcgggctg
    14521 tactgggcct gccccggcga agcgagaaca ccgtcttaca ttatgggcac cacggtaggt
    14581 atttgttcgg tgattggatt cctgccggat gtcttcgttt acccgattat cggccactgg
    14641 caagacaccc tgcccgctgc agaagcctac cgcaatatgt ggctgatggg catggcggcg
    14701 ctcggcatgg tgattgtctt tacctttttg ctgttccaaa aaattcgtac tgctgatagc
    14761 gcccccgcaa tagctagcag caagtaagcc aggaaaatta caggagaaaa atcatgtcga
    14821 aaaaatacat catagggatt gatggcggaa gtcagagcac aaaagtggtg atgtacgatc
    14881 tggaaggtaa tgtggtttgc gaaggtaaag gcttattaca gccgatgcac acgcctgatg
    14941 ccgatactgc agaacatcct gacgacgatt tatgggcatc attatgtttt gcaggtcacg
    15001 atttgatgag ccagtttgcc gggaataaag aagatattgt tggcatcggc ctgggctcca
    15061 tccgttgctg ccgtgcgtta ttgaaagccg acggcacgcc agctgcgccg ttgattagct
    15121 ggcaggatgc acgcgttaca cgcccttacg aacatacgaa tcctgacgtg gcgtatgtaa
    15181 cctctttttc gggttatctg acgcatcgct taaccggcga atttaaagat aatatcgcca
    15241 actattttgg tcagtggccg gtggattata agacctgggc atggagcgaa gatgccgcag
    15301 tcatggagaa gtttaatatc ccccgtcata tgctgtttga tgtgcaaatg ccaggcactg
    15361 ttcttgggca tatcacacct caagccgcgc tggcgacgca tttcccggca ggactgccgg
    15421 ttgtttgtac caccagtgat aaaccggtag aagctctggg agccggatta ctggatgatg
    15481 aaactgcggt aatttctctt ggcacttaca tcgcattgat gatgaacggc aaagccttgc
    15541 cgaaagatcc ggtggcgtac tggccgatta tgtcttctat tccgcaaaca ttgctgtatg
    15601 aaggttacgg tattcgcaaa ggcatgtgga cggtgagctg gctgcgcgac atgttaggcg
    15661 agtcgttaat tcaggatgcc agggcgcagg atctttcacc ggaagattta ctcaacaaga
    15721 aagcctctag tgtgccacct ggctgtaatg ggctgatgac ggtgctggac tggctgacca
    15781 atccgtggga accgtacaaa cgcgggatta tgatcggctt tgattccagc atggattacg
    15841 catggatata tcgttcgata ttggaaagcg tggcgctgac gctgaagaac aattacgaca
    15901 atatgtgtaa tgaaatgaat cactttgcga aacatgtaat cattactggc ggcggttcga
    15961 acaggatctg tttatgcaga tttttgccga cgtgttcaac cttccggcac gccgtaacgc
    16021 cattaacggt tgtgcaagcc tgggggcagc gattaataca ggggtaggtc tggggctata
    16081 cccggattac gcaacggctg tcgataaaat ggttcgcgtg aaagatatct ttataccgat
    16141 tgagagcaat gccaaacgct acgacgcgat gaataaaggc attttcaaag acctaaccaa
    16201 acatactgat gtgatcctga aaaaatcgta tgaagtgatg catggggaat tggggaattg
    16261 gggaatgcgg attcgatcca gagctggtcg aatgcgtgaa taaggtaggc cgggcggaaa
    16321 actgcccggc ctgaacatac ctgaatggtt atccccgctg gcgcggggaa cataagttcg
    16381 acaacttaaa aaatgataac aagtccggtt tatccccgct gatgcaggga acataataaa
    16441 aactttaact agttcggttt atccctgttt ttacaaggaa
//
LOCUS       DAEEKQ010000082        16367 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.082, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000082 DAEEKQ010000000
VERSION     DAEEKQ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16367)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16367)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16367
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.082"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            250..750
                     /gene="tssB"
                     /locus_tag="KLK57_002531"
     CDS             250..750
                     /gene="tssB"
                     /locus_tag="KLK57_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HBE0892871.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GLVSNK"
     gene            785..1009
                     /locus_tag="KLK57_002532"
     CDS             785..1009
                     /locus_tag="KLK57_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0892872.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFYEFQQSASHWQRVLH"
     gene            1060..2535
                     /gene="tssC"
                     /locus_tag="KLK57_002533"
                     /pseudo
     CDS             1060..2535
                     /gene="tssC"
                     /locus_tag="KLK57_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
     gene            2542..2955
                     /gene="tssE"
                     /locus_tag="KLK57_002534"
     CDS             2542..2955
                     /gene="tssE"
                     /locus_tag="KLK57_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HBE0892873.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2959..4809
                     /gene="tssF"
                     /locus_tag="KLK57_002535"
     CDS             2959..4809
                     /gene="tssF"
                     /locus_tag="KLK57_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HBE0892874.1"
                     /translation="MEFEERYFREELDYLRQLSKLLATEKPHLARFLAEKDADPDIER
                     LLEGVAFLTGNLRQKIEDEFPELTHGLIKMLWPNYLRPVPAMTLIEYTPDMDKSSVPV
                     LIPRNEQFTTNAGEIRVDEVLPSDAKKEEPPPCTFTLCRDIWLLPVRLEQIENRSTTR
                     NGVINITFSVAPGTDFRTLDLNKLRFWLGNDDNYTRDQLYLWFCEYLQGADLTVGEQH
                     IRLPEFMLKAVGFEPQDAMLPWPKNVHSGYRILQEYFCYPDAFLFFDLCGCPALPDGL
                     QAEFFTLQLRFSRPLPVDIRLRRDSLRLYCAPAINLFIHHAEAITLDNRRADYPLVPS
                     RHYPQHYDVFSVNSVVSQVQDMFRKKDLGRPVSTQAARQWPAFESFSHQIEYSRKREV
                     VYWHHRTKTSLFHRGFDHTLAFIHADGSYPSDESLLSNEVVSVSLTCTNRELPSQIRS
                     GDITGTTGKNAAVASFRNITRPTQPLWPVIDGSLHWPLLSAMNLNYLSLLDTDALKQV
                     IANFDRHAIHHPQTARLSQQKLDAIERLETRPVDRLFTGIPVRGLASTLYLHPEPFVC
                     EGEMYLLGTVLSHFLSLYASVNSFHMLTVVNTESQETWKWTERIGQHPLI"
     gene            4773..5855
                     /gene="tssG"
                     /locus_tag="KLK57_002536"
     CDS             4773..5855
                     /gene="tssG"
                     /locus_tag="KLK57_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HBE0892875.1"
                     /translation="MDGKNRAASSYLSPGNPPADKEQNDPLAQVFHNACSYNFFAMAE
                     LLHRLAKGEKGTPELSLRDDPAQETLRFSADASLAFPCSDISALKRDTSGAFRMTTTF
                     MGLQGSQSPLPGYYLAHLAWKAVHEQSPVGDFLDMFSHRLTQFVWHIWRKYRYHISFR
                     NGGVDAFSQRMYSLVGLGHRQLRDKLAINHSKMLAYSGILANPGRSPEIICGLVSHCF
                     DLSEVTLQNWQRRKVDIEPDQQNSLGSYSLKNGEKLAGRSVLGNFVLGTRVPDLSGKF
                     QLSITSLTRKQFLSFLPSGENFLPLTMFVSFILRDQLAWDLHLGLAPEQVGAMRLGDN
                     KSALLGWTSFLGTPEERPSVTIRVRS"
     gene            5880..7160
                     /gene="tagH"
                     /locus_tag="KLK57_002537"
     CDS             5880..7160
                     /gene="tagH"
                     /locus_tag="KLK57_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HBE0892876.1"
                     /translation="MPEEKLQTLSLQVINGSELESGRAARCLFTQQGNVGHGPECHWS
                     VQDRQQSIPAQAFTVILHDGTFCLRPQTAQLWLNQAKVTATSDLIQLRQGDEIQIGRL
                     MVRVHLNRGDIPHYDEEMATPETIVTNRDMLTDTLLSTEGAPHYPGMTHRHQLADTVV
                     NGFSADPLQALQSESLITTGDPLSGIAAVRPSAPLSDPASNGGINTPFMDLPPIYASP
                     GDRNDDVSAAEMAQRHVAVTPLLRGLGGSLTMSNSDDADDFLEEAGRTLQAAIKGLLD
                     LQQQRNSLSDKHLRPLEDNPLRLNMDYATALDVMFAEGKSPVHLAAPAAVSESLRNVR
                     HHEEANRAAIVESLRVLLDAFSPQNLLRRFVQYRRSHELRQPLDDAGAWQMYSHYYEE
                     LASDRQQGFEMLFNEVYAQVYDRVLREKQREPEA"
     gene            7157..7681
                     /gene="tssJ"
                     /locus_tag="KLK57_002538"
     CDS             7157..7681
                     /gene="tssJ"
                     /locus_tag="KLK57_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system lipoprotein TssJ"
                     /protein_id="HBE0892877.1"
                     /translation="MNRKAFLACVLMCVILTGCETAKKISEVIKNPDIQVGSLKEQPS
                     EITVTLLTEPDTNTNAEGESAAVDVQLVYLTDDSKLLAADYDQIASTPLPDVLGKNYI
                     DHQDFNLLPDTIKTLPPVKLDEKTQFIGVVAYFSDDQATEWKQIETVEGIGHHYRLLV
                     HVRQSSIEMKKEDE"
     gene            7684..9013
                     /gene="tssK"
                     /locus_tag="KLK57_002539"
                     /pseudo
     CDS             7684..9013
                     /gene="tssK"
                     /locus_tag="KLK57_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308252.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
     gene            9018..9779
                     /gene="icmH"
                     /locus_tag="KLK57_002540"
     CDS             9018..9779
                     /gene="icmH"
                     /locus_tag="KLK57_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IVB secretion system protein IcmH/DotU"
                     /protein_id="HBE0892878.1"
                     /translation="MDEGSLSLPPFTGHDEKSQRNYHLALRGNSLNPMIDAATPLLGM
                     VMRLSTMNSQTMPEHLFAQVVTDVQAVEQLLQEQGYEPGVIISFRYILCTFIDEAALG
                     NGWSNKNEWIKQSLLVHFHNEAWGGEKVFILLERLIREPKRYQDLLEFLWICFSLGFR
                     GRYKVAAQDQGEFEQIYRRLYHVLHKLRGDAPFPLLHQDKKTQGGRYQLISRLTVKHI
                     FCGGVVVLALFYLFYLLRLDSQTQDILHQLNKLLR"
     gene            9788..12565
                     /gene="tssH"
                     /locus_tag="KLK57_002541"
                     /pseudo
     CDS             9788..12565
                     /gene="tssH"
                     /locus_tag="KLK57_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
     gene            12562..13305
                     /gene="tagO"
                     /locus_tag="KLK57_002542"
     CDS             12562..13305
                     /gene="tagO"
                     /locus_tag="KLK57_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
                     /protein_id="HBE0892879.1"
                     /translation="MSRLRVFRQTGWFIAGLMTGLPATAAPAEASSMAGVAVAVATTT
                     PPDATATLQAMQSCRRESAALERLDCYDRLLAPLSPSGFDGALVKAGFVGEAWTRATE
                     QEKRREGNTTELLVTQVPGERPTVVITTPAIGHVPPRPVLMFSCVDNITRMQVALMHP
                     LDVHDIAVTLNADNRALRSHWFVRENGTLLESSRGLSGIDEIKQLFGAKTLTVDTGAD
                     NAAGKLTFNIDGLARAIAPLRDACHWAGE"
     gene            13310..14722
                     /gene="tssA"
                     /locus_tag="KLK57_002543"
     CDS             13310..14722
                     /gene="tssA"
                     /locus_tag="KLK57_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssA"
                     /protein_id="HBE0892880.1"
                     /translation="MAMDLRDPNVWISHLLENLPEEKLASALKDDNPNWEYIDGEIVK
                     LGSLAHSQLDIPELQRRGLVILASESKDFRLLAHLLRTLQHAGDPHLALRLLALYVEH
                     YWAVAAPQNMAHKKRFASQVIKRFETGIEVFSQNAATAQRDALSGELAKLAQCWQSHN
                     APELAQATDDLFALYQRAFNRAAPAPVPTPAASGSSPQTTATSESGVTQPSAPAPQIV
                     IDSHDDKAWRDTLLKVAAILCERQPDSPQGYRLRRHALWQNITSTPQAESDSRTPLAA
                     VSADMVADYHAQLGSADMALWQQVEKSVLLAPYWLDGHCLSAQTALRLGYKQVADAIH
                     DEVIRFLERLPQLTGLLFNDHTPFISEQTKQWLAASPDAKVAPVAQIGEESKAARACF
                     AEQGLEAALRYLDMLPEGDPRDQFHRQYLAAQLTEEAGLVQLAQQQYRMLFRMGLQMM
                     VADWEPSLLEQLEQKFTAEQ"
     gene            14741..>16367
                     /gene="tssM"
                     /locus_tag="KLK57_002544"
     CDS             14741..>16367
                     /gene="tssM"
                     /locus_tag="KLK57_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HBE0892881.1"
                     /translation="MFRLPTPRLLSGLKSALRPAMPRFKVSAFWLLILAWIFLLVWIW
                     WKGPMWTLYEEQWLKPLANRWLATAAWGIIALVWLTVRVMKRLQQLEKMQKQQREEAV
                     DPLSVELNAQQRYLDRWLLRLQRHLDNRRFLWQLPWYMVIGPAGSGKTTLLREGFPSD
                     IIYAPEGARGAEQRLYLTPHVGKQAVIFDIDGTLCAPADADILHRRLWEHALGWLKEK
                     RARQPLNGIILTLDLPDLLTADKRRREHLLQTLRSRLQDIRQHLHCQLPVYVVLTRLD
                     LLQGFAALFQSLNRQDRDAILGVTFTRRAHENDDWRTELNAFWQTWVDRMNLALPDLM
                     VAQTHTRASLFSFSRQMQGSREPLVSLLEGLLDGENMNVMLRGVYLTSSLQRGQMDDI
                     FTQSAARQYRLGNNPLASWPLVDTAPYFTRSLFPQALLAEPNLATESRAWLIRSRRRL
                     TVFSATGGVAALLLITGWHHYYNGNYQSGITVLKQAKAFMDVPPPQGEDDFGNLQLPL
                     LNPVRDATLAYGDWGDRSRLADMGLYQGRRIGPY"
BASE COUNT         3748 a         4537 c         4547 g         3535 t
ORIGIN      
        1 aaattattgc cataatacac ctgaagcgaa ttgcacaggt tcacacaatc tatttaacgc
       61 ttgcagaata cccctcttta cgatcgataa aaagtccttt tcttatttat tttgaagcgt
      121 aaaatgcggg gtgatgaagg agtggtgatt ttattttcac cgccattagg gcggcattgc
      181 cgatattttt ttgcggtaca aatatgccgt gttcatggcc tgtacgagat aacatgacgg
      241 atcactttta tgagcaaaaa atttgaaggc agcgtcgcgc cccgcgagcg tattaatata
      301 tcgtatgtcc caaaaacaga tggccagacg gctgaagtgg aacttccgct taacatgctc
      361 gttgtcggcg ataccggaaa tacgcaggaa acatccccac tggatgaacg tcaggcagtt
      421 tccgttaaca agcacaattt cggtgcagtg atggctgaag cagccattgg cctcaatttt
      481 actgtccccg ccacgctgaa gggaagcaca acagacgatg aactgaatgt ggcgctgaat
      541 atcaaatcac tggatgattt ttcaccagat agcgtggccc ggcaggttcc ggaagtgaat
      601 aaattgctgg aactgcgtga agcactgact gcactgaagg ggccgatggg taaccttccg
      661 gcattccgta cccagctcca ggcattgctg gaaaatgaag agtcccgcga gcagttgctc
      721 aaagaaatcg gtctggtgag caataaataa ccaccactat ttcattaaac agggttatcc
      781 aggcatgacg gcacacaaaa atatctccgg gacataccac ctctctgttg cagacattct
      841 gcaggtggtt taccaggtat gtttcagccc gtccgttgaa atcaaccagg atggtgtggc
      901 tgcgctgatt acaacactgg atcgccgtat cagtgatcta ttggatgaga ttatccattt
      961 ttatgagttt cagcagagcg cgtcgcactg gcagcgcgta cttcactgat ggcgaaaagc
     1021 cggtttcaga aacacagcta aaacagaagg attttcagta tgtctttaca ggaagaggaa
     1081 ctcgtttcca gtcatgccgg gcagcctgag caggcatcgt cgctgcttga tcaaatcatg
     1141 gcacagaccc gtattcagcc cggttcggaa ggttatgacg ttgccctcca gggtgtgaca
     1201 gcctttattg ccagcatcct gcaatccact gcatctgccg aaccggtcaa taaactggcc
     1261 gtggacagca tgattgcaga tattgatgaa cgcatcagcc gccagatgga tgtcatcatc
     1321 catgcgcctg cctttcagca ggtcgagtcg ttctggcgct cactgaaaac catggtggat
     1381 cgcgttgatt tccgtgaaaa catcaaggtc aacgtgcttc atgtcaccaa gcaggaactt
     1441 ctggaagact ttgagtttgc gccagaaatt attcagtccg gattttataa gcacgtttat
     1501 tcgtccggtt tcggacagtt tggcggtaaa cccatcgctg ccgtgctggg cgcttatgag
     1561 ttcaaaaata ccgcgcctga catgaaactg ctgcaatacg tcagtgccgt gggcgcaatg
     1621 gcgcacgcac cgttcctgtc ctccgtttcc ccggagttta tggggctgaa ctcgtggacc
     1681 gaactgccta atatcaaaga tctttatgcc atctttgaag gtccggccta caccaaatgg
     1741 cgcgccctgc gtgactcgga agattcgcgc tatttagggc ttacggcacc ccgcttcctg
     1801 cttcgccagc cctattcacc aacagataac cctgttaaga actttaacta ttacgaagat
     1861 gtcagccaga accacgaaga ttatctgtgg ggtaatacgg cctggatgct ggcatgcaat
     1921 atcgcagaca gttttgccaa ataccgctga tgtccaaaca ttatcggtcc gcaaagcggc
     1981 ggcgcagtga aagatctgcc gatgcatctg ttcgaaacga tggggcagat tcaggccaaa
     2041 atcccaaccg aggtgctggt caccgatcgc cgcgaatttg aactggctga agagggtttt
     2101 atcaccctta ccatgcgtaa agactctgat aatgcagcct ttttctctgc aaactcggta
     2161 caaaaaccga agcactttcc gggaaaagat gcagaaacca actataagct gggtacgcag
     2221 cttccttatc tcttcatcat caatcggtta gcgcactaca tcaaggtgtt gcagcgtgaa
     2281 caactggggt catggaaaga aaggagcgac ttagaaagag agcttaatac ctggatccgt
     2341 cagtatgttg ccgaccagga aaacccgcct gctgacgtgc gcagccgcaa acctctgcgc
     2401 gctgccaaag ttgaggttat ggatgtagaa ggcgaacccg gctggtacca ggttgcgcta
     2461 agcgtgagac ctcattttaa atttatgggg gcaaattttg agctttccct ggttggccgg
     2521 ttagacaggg agtaacacac catgataccg gatcgggaaa gaaccacagg ttctcttttt
     2581 gagcgtatgg aagcctcttc ggccagaaac cggcagggag gcagtattca ttcgctcagg
     2641 caatccattc gccagaacct acgaaacatt cttaataccc ggtccggtag ttgccggggt
     2701 gcgcctgagc tgggcattga tgaaccggaa ggggctgaaa acttccgcga atccatgagt
     2761 cgggccatcg agcaatgtat agaacgctat gaaccccgga tctcacacgc tgaagtgcag
     2821 gcagtggttt catccgcctc cagcccgctg gatatgacgt ttcatattac agcctgggtg
     2881 acatttaacg aaacgcatga ggtgcttgaa tttgatatgg caccgaatgg cagccagcat
     2941 taccgggtgg actgacaaat ggaatttgaa gaacgctatt tccgggaaga actcgattac
     3001 ctgcgccagc ttagcaagct gctggcaacg gaaaaacccc atctggcccg cttcctggcc
     3061 gaaaaagatg cagatccgga tattgaacgc ctgctggaag gggtggcttt tcttaccggc
     3121 aatctccgcc agaaaattga ggatgaattc ccagaactga cgcacgggct tattaagatg
     3181 ctatggccta attacctgcg tccggttccg gcaatgaccc ttattgaata tacgccggat
     3241 atggataagt cttctgtacc ggtgttaatc ccccgtaatg agcagtttac aaccaacgcc
     3301 ggggaaatca gagttgatga agtgctgccc tctgatgcta aaaaggagga gccgcctccc
     3361 tgtaccttca ccctctgccg ggatatctgg ctgctgcccg ttcgcctgga gcagattgaa
     3421 aaccgcagta cgacccgtaa tggtgttatc aacatcacct tttcggtcgc accgggaacg
     3481 gacttccgca cgctggatct gaacaaactt cgcttctggc tcggcaatga cgacaactat
     3541 acccgtgacc agctttattt atggttctgc gaatacttgc agggtgccga cctgactgtg
     3601 ggtgaacagc atattcgcct gcctgagttt atgctaaaag ctgtcggttt tgagccgcag
     3661 gatgccatgc tgccctggcc gaaaaacgtc cacagcggct accggatcct tcaggagtat
     3721 ttctgttacc ccgatgcgtt tctctttttt gatctttgtg gttgtccggc tttgcctgac
     3781 ggattgcagg cggaattctt taccctgcaa ctgcgttttt cgcgcccttt gcccgtggac
     3841 atccggctgc gccgcgattc cctgcgcctg tattgcgcac ctgccattaa tttatttatc
     3901 caccatgcag aagccatcac gctggacaac cggcgggcag actatccgct ggttcccagc
     3961 cgccattacc cacaacatta cgatgtattt tccgttaaca gtgtggtgag ccaggtccag
     4021 gatatgttca ggaaaaaaga tctggggcgt cctgtttcga cgcaggccgc gcgccagtgg
     4081 ccagcctttg aaagtttcag ccatcagata gaatacagcc ggaagcggga agtggtgtac
     4141 tggcatcacc ggaccaaaac atccctgttc catcgcggct ttgatcatac ccttgccttt
     4201 atacatgctg atggcagtta tccgtcagac gaatctctgc tcagtaatga agtggtttcg
     4261 gtatcgctga cctgtaccaa ccgtgagctt ccgtcacaaa ttcgttccgg cgatatcacc
     4321 ggcacaaccg gaaaaaatgc ggctgttgct tcatttcgca acattacccg cccgacgcaa
     4381 ccactctggc cggtcattga tggcagcctg cactggcccc tactctccgc catgaacctg
     4441 aattatctgt cattactgga tacggacgcg ctgaagcagg tcatcgccaa ctttgatcgc
     4501 cacgcaatcc atcatccgca gacggcacgg ctgtcacaac aaaagctgga tgccattgag
     4561 cgtctggaga cccgccccgt tgatcgcctg tttacgggta ttcccgtccg ggggctggct
     4621 tccacgctgt atctgcaccc ggagccgttt gtctgtgaag gggaaatgta tctgctcggt
     4681 acggtgcttt cgcattttct gtcgctgtac gccagcgtta actcattcca catgctgacc
     4741 gttgtgaaca cagaaagcca ggagacatgg aaatggacgg aaagaatcgg gcagcatcct
     4801 cttatctgag tccgggaaat cccccggcag ataaagagca gaatgatccg ctggcacagg
     4861 ttttccataa tgcctgctct tacaattttt ttgcgatggc ggagctgttg caccgcctgg
     4921 caaagggtga aaaggggacg ccagaattat ccctgcgcga cgatccggca caggaaaccc
     4981 tgcgtttcag cgccgatgcc agccttgcct tcccgtgcag tgatatcagt gcgctgaaac
     5041 gggatacgtc aggggcattc aggatgacca ccacctttat ggggttacag gggagccagt
     5101 cgcctctccc cggttattac ctcgctcacc tggcctggaa agcggtacat gaacaaagtc
     5161 cggtaggtga ttttctggat atgttcagcc accgcctgac ccagtttgtc tggcatatct
     5221 ggcgtaagta ccgctatcac atcagttttc gtaacggcgg tgtggatgcg ttctcgcagc
     5281 gcatgtactc ccttgtcggg ctgggccacc gccagcttcg cgataagctg gcgattaatc
     5341 acagcaaaat gctggcgtat tccgggatcc tggcaaatcc gggacgatcc ccggaaatta
     5401 tctgtggcct ggtgtcccac tgctttgatt tgtcagaagt gacactgcaa aactggcaac
     5461 gtcgcaaggt ggatattgaa ccggatcagc aaaatagtct ggggagctat tctcttaaaa
     5521 atggcgaaaa actggcggga agatcggtac tgggcaactt tgttctgggt acacgagtgc
     5581 ccgatctcag cggaaaattc cagctcagta ttaccagcct gacccgaaaa cagttcctct
     5641 cttttctgcc gtccggcgaa aacttcctgc cactgacgat gttcgtgtcc ttcatcctgc
     5701 gcgatcagct tgcctgggac ttacatctgg ggctggcgcc ggagcaggtg ggcgcaatgc
     5761 gcctgggcga taacaaaagt gcgctgctgg gctggaccag cttcctcggc acaccggaag
     5821 aacgaccctc agtcaccatc agggtacggt cgtaaattat gataatcacg gagcagaata
     5881 tgccggaaga gaaactacag acgctttcat tgcaggtcat taacggcagt gagctggaaa
     5941 gcgggcgggc ggcgcgctgt ctgttcacac agcagggaaa tgtcggccat ggccccgaat
     6001 gccactggtc ggtacaggat cgtcagcaga gcattccggc ccaggctttt accgttatcc
     6061 tgcacgatgg cacattttgt ctgcgcccgc agacggcaca actgtggctg aatcaggcaa
     6121 aagtcacagc aacatcagac ctaatacagt tgcgccaggg cgatgagatc cagatcggac
     6181 ggctgatggt gagggttcat ctgaaccggg gagatattcc ccattacgat gaggaaatgg
     6241 ccactcccga aaccatcgtt accaatcgcg atatgctcac ggataccctg ctatcaacgg
     6301 agggtgcgcc acactatccg ggaatgactc accggcacca gcttgcagac accgtggtaa
     6361 acggtttttc tgccgatcca ctccaggctc ttcagtccga aagcctgatt accacgggcg
     6421 atccgctttc aggcattgcg gctgtccggc catcggcacc gctgtccgat ccggcaagta
     6481 atggggggat caatactccg tttatggatc tgccgcccat ttatgccagc cccggcgatc
     6541 gtaatgacga cgtctctgcg gcagaaatgg cacaacgcca cgttgcggtc acccccttac
     6601 tgcgcggtct tggcggctcg cttaccatga gcaattccga cgatgcggat gattttctgg
     6661 aggaggccgg acgaacgtta caggccgcaa taaaaggtct gctcgatttg cagcagcagc
     6721 gtaacagcct ctcagacaaa catttgcgcc cgctggaaga taacccgctg cgcctgaaca
     6781 tggattacgc caccgcgctc gacgtaatgt ttgccgaagg taaaagcccg gtacatctgg
     6841 cggctcccgc cgccgtcagt gaaagcctgc gcaatgtccg ccaccacgaa gaagctaacc
     6901 gggcagcgat tgtggagtcg cttcgtgtcc tgctggatgc tttctcacca caaaatctgc
     6961 tgcgccgctt tgtgcagtac cgccgcagcc atgaactgcg ccagccgctg gatgatgccg
     7021 gagcatggca aatgtacagc cattattacg aagaactggc ctccgatcgc cagcaggggt
     7081 ttgagatgct gtttaacgag gtctacgccc aggtctatga ccgggtgctt cgtgaaaaac
     7141 agcgggagcc ggaagcatga acaggaaggc gtttctggcc tgcgtgctga tgtgcgtcat
     7201 attaaccggc tgcgaaacgg cgaaaaaaat cagtgaggtg atcaaaaacc cggatatcca
     7261 ggtcgggagc ctgaaggagc aaccttcgga aataaccgtc accctgctga ccgaaccgga
     7321 caccaatacc aatgcggaag gtgaatcagc cgccgtggat gtgcaactgg tgtacctgac
     7381 tgacgactca aaattgctgg cagccgacta tgaccagata gccagcacgc cgctaccgga
     7441 tgtgctgggt aaaaactata tcgaccacca ggatttcaac ctgttgccgg acaccatcaa
     7501 aaccctgccg ccggtaaagc tggatgagaa aacgcagttt attggtgtcg tggcctattt
     7561 ctccgatgac caggccactg aatggaaaca aatcgagacg gtggaaggaa tcggccacca
     7621 ttaccgcctg ctggtgcatg tacgtcaaag cagcatcgaa atgaaaaaag aggatgaata
     7681 attatggcaa ccacccgcaa caaggtgatg tggcaggaag ggatgctgat gcgtccacac
     7741 catttccagc agcagcagcg ttacaacgac tacctggata accagcgttt ccgggccatg
     7801 aatgatttat cctggggatt taccgaactc accctcaaca atgaactgct ggcgcagggt
     7861 aagatcatga ttgacagcgc gtcaggcaca ctgcccgacg gcaccgtctt ttctatcccc
     7921 gaccaggacg cactgcccga tccgctgcac ccgcaaaatt ttccggacga aagaagccgc
     7981 aatatctacc tcgctctgcc ggtcgccagt gatgtgagaa atgaaatcag cgacggacgg
     8041 cgaatcgggc gttaccggct gaattatgcc gatgtccggg atttgcattc agaagaaggt
     8101 gacgcgcgaa cattgacgct gggtcaactg acgccgcgca ttatgagcgg tgcagaagat
     8161 atgagcgcct atattacgct cccgctttgc cgtatcagcg atcgccgccg atggctccct
     8221 gacgttggat gacgatttta tcccctcctg ccagaatatt caggtcagta agaaactgcg
     8281 tgtttatctc aaagaggtac agggggccat tggcggacgg gcaagcgatc tggcaaaccg
     8341 tattggctct ccggcgcaga gcggcatcgc ggatgtggcg gaatttatga tgttgcagtt
     8401 acttaaccgt aaccagaccc ggtttaccca tcacgcccgt cgatcccagc tccacccgga
     8461 agatttctac cttgatcttg ccgggttgct gggtgaactg atgaccttta ccgagccgtc
     8521 gcgcctgccc tgcccgcttg atgtgtatga tcatcatgac ctgaccaaaa tatttaaaac
     8581 gctgttaccg gaagtcaaac gggcgctgca taccgtactg tcgccaagag ccgtcaatct
     8641 gccgctgcat ctgcgtgacg gtatctggca ggccgatatc catgacacag aactgctgca
     8701 atctgccacc tttgtgctgg ccgtggcggc aaacatgccg gtcgatcaga tccagcgcca
     8761 gtttatccag cagtcgaaaa tttcctcgcc ggaaaaaatc cgcaatatgg tcagtgtgca
     8821 gatccccggt attccattgc gtgcccttat ggtggctccc cgccagcttc cttaccattc
     8881 cgggttcagc tatttcgaac tcgacaagag tggccaggcc tggacagaaa tggctgccgc
     8941 cggggccgtt gcactgcatg tttccggcag tttcccggat ctgaacatgc aactgtgggc
     9001 gataagaggg taagacgatg gatgaggggt cactgtctct gccaccattt accggacacg
     9061 acgaaaaaag ccagcgcaac taccatctcg cgcttcgtgg caacagcctg aatccgatga
     9121 tcgacgcggc cacgccgcta ctcggtatgg taatgcgcct ttcaaccatg aacagccaga
     9181 cgatgccgga gcatcttttt gctcaggtgg tgacagatgt tcaggccgtg gagcaactgc
     9241 ttcaggagca gggctacgaa ccgggcgtga ttatctcgtt tcgctacatc ctctgcacgt
     9301 ttattgatga agcggcgctc ggtaacggct ggtcaaacaa gaacgagtgg atcaagcagt
     9361 cattgctggt ccatttccac aacgaagcct ggggtggcga gaaagttttc atcctgctgg
     9421 agcgcctgat ccgtgaaccc aaacgttacc aggatctgct ggaatttctc tggatctgtt
     9481 tttctctggg atttcgcggg cgctacaaag tggcagcaca ggatcagggg gaatttgagc
     9541 agatctaccg ccgtctgtac cacgttctgc ataaactgcg cggcgatgcc cctttcccgc
     9601 tgttacacca ggacaaaaaa acccagggcg ggcgttatca gctcatcagt cgcctgactg
     9661 ttaaacatat tttttgtggt ggcgttgtcg tgctggcgtt gttttacctg ttctatctgt
     9721 tgcggctgga cagccagacg caggacatcc tgcaccagct aaacaaatta cttcgataag
     9781 gacgtttatg atccagattg atcttcccac gctggtaaaa cggctgaacc tgttctcccg
     9841 ccaggcgctg gagatggccg cctctgaatg tatgagtcag caggcagcgg aaattaccgt
     9901 cagccatgtg ctcattcaga tgctcgccat gccacgcagt gacctgcggg ttattacccg
     9961 ccagggcgat attggcatgg aagagttgcg ccaggcgctg acggtagaga actacacaac
    10021 cgcccgttct gcggacagct acccggcgtt ttccccgatg ctggttgagt ggcttaaaga
    10081 gggctagctg ctggcgtcgg ctgagatgca gcacagcgaa ctgcgcggcg gcgtgttgct
    10141 gctggccctg ctgcattcgc cgctgcgtta tataccgcct gctgccgccc ggctgttgac
    10201 cggcattaac cgtgaccgtc tgcaacagga ctttgtgcag tggacacagg agtcggcgga
    10261 atcagtcgtg ccggatgcag acggtaaagg cgcaggcaca atgacggacg cctctgacac
    10321 cctgcttgcc cgctatgcca aaaacatgac cgcagacgcc cgtaacggca ggcttgaccc
    10381 ggtactgtgc cgcgaccacg aaatcgacct gatgatcgac attctctgcc gccgccgtaa
    10441 aaacaacccg gtggtggtgg gcgaagcggg cgtgggcaaa agcgcactga ttgaagggct
    10501 ggcgctgcgc atcgtggcag gccaggtgcc ggacaagctg aaaaacaccg atatcatgac
    10561 ccttgacttg ggcgcattgc aggccggggc gtcggtgaag ggtgaattcg aaaaacgttt
    10621 caaagggctg atggcggagg tcatttcctc cccggtgccg gtcattctgt ttatcgacga
    10681 agcacatacc ctgattggcg cgggcaacca gcagggcggg ctggatatct ccaacctgct
    10741 caaaccggcg ctggcgcgcg gcgagctgaa aaccatcgcc gccaccacct ggagcgagta
    10801 caaaaaatac ttcgaaaaag atgccgccct gtcgcgccgc ttccagttgg tgaaggtcag
    10861 cgaacccaac gctgccgaag ccaccattat tctgcgcggt ctgtcggcgg tctatgaaca
    10921 gtctcacggc gtgctgattg atgatgacgc cttgcaggcc gctgcgacat taagcgagcg
    10981 ttatctctcc gggcgtcagt taccggacaa agcgattgat gtgctggata ccgcctgcgc
    11041 ccgtgtggcc atcaacctgt cgtcgccgcc gaagcaaatc tcggcgctga ccactctgag
    11101 ccaccagcag gaggcggaaa ttcgccagct tgagcgcgag cttcgcatcg gactgcgtac
    11161 cgacacatca cggatgaccg aggtgctggt gcagtatgat gaaacgctga cggcgctgga
    11221 tgaactggaa gcggcctggc accagcagca gacgctggtc cgggagatta tcgcgctgcg
    11281 ccagcagtta ctgggcgtgg cagaggacga tgcggcgccg ttgccggacg cagataccgt
    11341 ggaggatacg cagccagagt cagagtcaga gtcagagtca gagcaggata ataccggtgc
    11401 cgaaccggct gatgaggccg acagagaaca accggaagag accgctgaaa cagtttcccc
    11461 ggtacagcgg ctggcccagc tcactgccga actggacgcc ctgcataacg accggttgct
    11521 ggtctccccg cacgtcgata aaaaacagat tgcggcggtg attgccgaat ggaccggcgt
    11581 gccgctcaac cgcctgtcgc agaatgaaat gtcggtcatc accgacctga cgaaatggct
    11641 gggtgacacc atcaaaggcc aggacctggc gattgccagc ctgcataagc atctactgac
    11701 cgcacgcgcc gacctgcgtc gtccgggacg cccgctcggt gcgttcctgc tggctggccc
    11761 cagcggtgtg ggtaaaaccg aaaccgtcct gcaactggca gagctgctct acggcggtcg
    11821 ccagtacctg accaccatca atatgtccga attccaggag aaacacaccg tctcgcggct
    11881 gattggttcg cctccgggct acgttggcta cggtgaaggc ggcgtactga ccgaagcgat
    11941 tcgccagaaa ccctactcgg tagtactgct cgatgaagtg gaaaaagcgc acccggatgt
    12001 gctcaacctg ttctaccagg cgttcgataa gggcgaaatg gcagacggtg aaggccgcct
    12061 gattgactgc aaaaatatcg tcttcttcct gacgtccaac ctcggctacc aggtaatagt
    12121 cgagcatgcc gatgacccgg aaaccatgca ggaagtacag tatccggtgc tggccgactt
    12181 cttcaaacct gccctgctgg cgcgtatgga agtggtgccg tatctgccgc tgtcgaaaga
    12241 gacgctcgcc accattatcg ccggaaaact ggcccgtctg gataacgtgc tgcgcagtcg
    12301 ctttggtgta gaagtggtca ttgaaccgga agtgacggac gaaatcatga gccgcgtcac
    12361 ccgcgcggaa aacggcgcga ggatgctgga atcggtcatc gatggcgaca tgctaccgcc
    12421 gctctcgctg ctgctgttgc agaaaatggc ggctaacacg gcgattgccc ggattcggtt
    12481 gtcggcagtg gacggcgcat ttacggcaga cgtggaagat gctcagaacg acgagtccgt
    12541 cacaaaggat gaaacggttt tatgagcagg ttgcgcgttt tccggcagac aggatggttt
    12601 atcgcgggcc tgatgacggg cttacccgcc actgccgcgc ccgcagaagc atcgtcaatg
    12661 gccggtgtgg cggtcgccgt cgccaccacg accccgccgg atgcgacagc cacgttacag
    12721 gccatgcagt cctgtcggcg ggagtccgcg gcgctggagc ggcttgactg ctatgaccgc
    12781 ctgctggcac cgctgtcccc gtcagggttt gacggggcgc tggtcaaagc cggttttgtg
    12841 ggcgaggcct ggacccgcgc cacagagcag gaaaaacgcc gcgagggcaa caccacagaa
    12901 ctgctggtga cgcaggtgcc gggggagcgc ccgacggtgg tgataaccac cccggctatc
    12961 ggtcatgttc cgccacgccc ggtgctgatg ttcagttgcg tggacaatat cacccggatg
    13021 caggtggcgc tgatgcaccc tctggatgtc catgatatcg ccgtcactct gaatgccgac
    13081 aaccgggcat tgcgcagcca ctggtttgtg cgcgagaacg gcaccctgct ggagtccagc
    13141 cgggggttat ccgggattga tgaaattaag caactgttcg gcgcaaaaac gctgacggtg
    13201 gataccggcg cagacaatgc cgccggaaag ctgaccttta acattgacgg gctggcaagg
    13261 gcgattgctc ccctgcgcga tgcctgccac tgggcgggag aataaaaata tggcaatgga
    13321 cttacgcgat ccgaatgtct ggatatcgca cctgctggaa aacctgccgg aagagaaact
    13381 ggcatcggcg ctgaaagatg acaacccgaa ctgggagtat atcgacggcg aaatcgtcaa
    13441 gctgggatcg ctggcccaca gtcaactgga tatcccggaa ctccagcgcc gggggctggt
    13501 cattctggct tcggagagta aggattttcg tctgctggcg catctgctaa gaaccctgca
    13561 acacgccggt gatcctcatc tcgcattgcg cctgctggcg ctgtatgtgg agcattactg
    13621 ggcggtggct gcgccgcaga atatggcgca taaaaaacgc tttgccagcc aggtcattaa
    13681 acgctttgaa acaggtattg aagttttttc acagaacgcc gccacagcgc agcgcgatgc
    13741 gctgtcaggg gaactggcaa aactggcgca atgctggcag tcacataacg ccccggaact
    13801 ggcacaggct actgatgatc tttttgccct gtaccagcgt gcgtttaatc gtgcggcacc
    13861 ggctcctgtc cccactccgg cggcctccgg tagttcacca cagaccaccg ccacgtctga
    13921 aagcggcgtg acgcaaccca gtgctccggc tccccaaatc gtcatcgaca gtcacgacga
    13981 caaagcctgg cgcgacacgc tgttaaaagt ggcggccatt ttatgtgaac gccagccgga
    14041 ttcaccgcag ggctaccgtc tgcgccgcca tgcgctttgg cagaacatca ccagtacgcc
    14101 ccaggcggaa agcgatagcc gtactccact ggctgcggtc tccgccgata tggtggcaga
    14161 ttaccatgct cagctcggta gtgccgatat ggcgctgtgg cagcaggtgg agaaaagcgt
    14221 actgctggct ccttactggc tggatggtca ctgcctttct gcacagacgg cgctgcgtct
    14281 gggttacaaa caggtggcag acgccatcca cgatgaggtc atccgcttcc ttgagcgtct
    14341 gccccagctt accgggctgc tgtttaatga ccacacaccg tttatcagtg agcagacgaa
    14401 acaatggctg gcagcttcgc ccgacgccaa agttgcaccg gttgcgcaaa tcggtgagga
    14461 atcgaaggca gccagagcct gttttgctga gcagggtctg gaggcggcgc tgcgatatct
    14521 ggacatgcta cccgaaggcg atccccgcga tcagtttcac cgccagtacc ttgccgcaca
    14581 gttgacggaa gaggcggggc tggtacagct tgcgcagcag cagtaccgga tgttgttcag
    14641 gatgggattg cagatgatgg tggcagactg ggagccgtcc ctgcttgagc agctagaaca
    14701 aaaattcacg gcagaacaat aaaagagaac aggagttttt gtgttcagat taccaacacc
    14761 ccgactactc agcggactca aatcagccct gcgaccggcg atgccccggt ttaaagtctc
    14821 tgctttctgg ctgctgatac tggcgtggat ttttctgctg gtgtggatct ggtggaaagg
    14881 cccgatgtgg acgctgtatg aagaacagtg gctcaaacca ctggcgaacc gctggctggc
    14941 aacggcggcg tgggggatta ttgccctggt gtggcttacc gtccgggtga tgaagcgcct
    15001 gcaacagctt gaaaaaatgc aaaagcaaca gcgcgaggaa gccgttgatc cgctcagcgt
    15061 ggaactgaac gcccagcagc gttatcttga ccgctggctg ctgcgcctgc aacgccatct
    15121 cgacaaccgc cgtttcctgt ggcagttgcc gtggtatatg gtcatcggcc cggcgggcag
    15181 tggcaaaacg acactgctgc gcgaggggtt tccgtccgac attatttatg ccccggaggg
    15241 cgcacgcggc gcagaacaac gcctgtacct cacgccccat gtcggtaaac aggcggtgat
    15301 ctttgatatc gacggcacac tctgcgctcc cgctgatgcg gatatcctgc atcgccgtct
    15361 gtgggaacat gccctcggct ggctgaaaga aaagcgcgcg cgccagccgc tgaacgggat
    15421 tattctgaca ctcgatttac ccgatctgct gaccgcagac aaacgccgcc gcgagcatct
    15481 gttacagacg ctgcgcagcc gcttacagga tatacgccag catcttcact gccagttacc
    15541 ggtttacgtg gtactgaccc ggcttgattt attacagggt tttgccgccc tgttccagtc
    15601 actcaacaga caggatcgcg atgcgattct gggcgtcacg ttcacccgcc gtgcccatga
    15661 aaatgatgac tggcgaacag agttgaatgc tttctggcag acatgggtgg atcgaatgaa
    15721 tctggcgttg ccggatctga tggtcgctca gactcacacc cgcgcgtctt tattcagttt
    15781 ttcccgccag atgcagggaa gccgtgaacc gctggtgtca ctgcttgagg gtctgcttga
    15841 tggcgaaaat atgaacgtga tgctgcgtgg tgtctatctc acctcttcgc ttcagcgtgg
    15901 acagatggat gatatattca cccagtctgc cgcccgccag taccggctgg gcaataaccc
    15961 actggcgtcc tggcccctgg tggacaccgc gccttatttc acccgcagcc tgttcccgca
    16021 ggcattactc gcagagccta atctggcaac agagagccgc gcctggctga tacgttcccg
    16081 tcgccgcctg acggttttct ctgccacagg cggcgtggca gcactgctgc tcatcaccgg
    16141 ctggcatcac tattacaacg gtaactatca gtccggcatc accgtgctta agcaggccaa
    16201 agcctttatg gacgtgccgc ctccgcaggg ggaagatgac tttggcaacc tgcaactgcc
    16261 gctcctgaac ccggtacgcg atgccacact ggcctatggc gactggggcg accgcagccg
    16321 tctggccgat atgggactgt accagggacg acgtatcggg ccttatg
//
LOCUS       DAEEKQ010000083        16341 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.083, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000083 DAEEKQ010000000
VERSION     DAEEKQ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.083"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            429..1646
                     /gene="alaA"
                     /locus_tag="KLK57_002545"
     CDS             429..1646
                     /gene="alaA"
                     /locus_tag="KLK57_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HBE0892882.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1730..2329
                     /gene="yfbR"
                     /locus_tag="KLK57_002546"
     CDS             1730..2329
                     /gene="yfbR"
                     /locus_tag="KLK57_002546"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HBE0892883.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVNMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2388..4220)
                     /locus_tag="KLK57_002547"
     CDS             complement(2388..4220)
                     /locus_tag="KLK57_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HBE0892884.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4307..4955)
                     /gene="hxpA"
                     /locus_tag="KLK57_002548"
                     /pseudo
     CDS             complement(4307..4955)
                     /gene="hxpA"
                     /locus_tag="KLK57_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416796.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
     gene            complement(4966..5460)
                     /locus_tag="KLK57_002549"
     CDS             complement(4966..5460)
                     /locus_tag="KLK57_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HBE0892885.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5543..5998)
                     /locus_tag="KLK57_002550"
     CDS             complement(5543..5998)
                     /locus_tag="KLK57_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HBE0892886.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6336..7538
                     /gene="ackA"
                     /locus_tag="KLK57_002551"
     CDS             6336..7538
                     /gene="ackA"
                     /locus_tag="KLK57_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HBE0892887.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7613..9757
                     /gene="pta"
                     /locus_tag="KLK57_002552"
     CDS             7613..9757
                     /gene="pta"
                     /locus_tag="KLK57_002552"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HBE0892888.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9947..11467
                     /gene="yfcC"
                     /locus_tag="KLK57_002553"
     CDS             9947..11467
                     /gene="yfcC"
                     /locus_tag="KLK57_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HBE0892889.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATMIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGTRMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11500..12042)
                     /gene="yfcD"
                     /locus_tag="KLK57_002554"
     CDS             complement(11500..12042)
                     /gene="yfcD"
                     /locus_tag="KLK57_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HBE0892890.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(12100..12651)
                     /gene="yfcE"
                     /locus_tag="KLK57_002555"
     CDS             complement(12100..12651)
                     /gene="yfcE"
                     /locus_tag="KLK57_002555"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HBE0892891.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12707..13351)
                     /gene="yfcF"
                     /locus_tag="KLK57_002556"
     CDS             complement(12707..13351)
                     /gene="yfcF"
                     /locus_tag="KLK57_002556"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HBE0892892.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLKPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RRIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13487..14134
                     /gene="yfcG"
                     /locus_tag="KLK57_002557"
     CDS             13487..14134
                     /gene="yfcG"
                     /locus_tag="KLK57_002557"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HBE0892893.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLTEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14191..14553
                     /gene="folX"
                     /locus_tag="KLK57_002558"
     CDS             14191..14553
                     /gene="folX"
                     /locus_tag="KLK57_002558"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HBE0892894.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14574..15467
                     /locus_tag="KLK57_002559"
     CDS             14574..15467
                     /locus_tag="KLK57_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HBE0892895.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVFISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNDLRG
                     PFNMVSPYPVRNEQFAHALGQALHRPAILRVPATAIRLLMGESSILVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15515..>16341)
                     /gene="rpnB"
                     /locus_tag="KLK57_002560"
     CDS             complement(15515..>16341)
                     /gene="rpnB"
                     /locus_tag="KLK57_002560"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HBE0892896.1"
                     /translation="TARDFIDIHLPAPLRKLCDLTTLKLESNSFIDEDLRQYYSDLLW
                     SVKTQEGVGYIYVVIEHQSKLEELMAFRMMRYSIAAMQNHLDAGYKELPLVIPMLFYH
                     GCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDITVVPDDEIMQHRKMALLELIQKH
                     IRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQTGDAQRFRAFIGEITERAPQEK
                     EKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLDRELVMMVTRLSPDDLIAQSH"
BASE COUNT         4087 a         4342 c         3982 g         3930 t
ORIGIN      
        1 tgaatattaa ataataatta attagcatca tcctcattca ttaattccgc ttaacaatag
       61 tttcacacct tgcccccatt gcgacacctc ggaaatcatc acgctgttaa actctccgta
      121 atgcggaaat atcatcatcg cgtttcatta gtgaattctt aaatgaggca ttttcacaca
      181 attatcttac agataaaaaa accagacttg caattaagaa tcagaacaat tcactatata
      241 acattgcatg caaagcatat acgcctcatt attttgtcat tattaagtta ttaacagcac
      301 aatcgagcct tcccctctgg caaaatctta ttctgcagac cttcaaaaca ccgtcctggg
      361 ggagtacatt gttctaagct gacttccacg gcagggagtg gcgataacag caaaaaaggt
      421 caagattcat gtcccccatt gaaaaatcca gcaaattaga gaatgtctgt tatgacatcc
      481 gtggtccggt gctgaaagaa gcaaaacgcc tggaagaaga aggtaacaag gtactgaaac
      541 tgaacatcgg caacccagcc ccattcggtt ttgacgcgcc agatgaaatc ctcgttgacg
      601 tgatacgcaa cctgcctacc gctcaagggt attgcgattc caaagggctt tactccgcgc
      661 gtaaagccat catgcagcac taccaggctc gtggcatgcg tgatgttacc gtggaagata
      721 tttacatcgg caatggtgta tcggagctta tcgttcaggc aatgcaggca ttgctgaaca
      781 gcggggacga aatgttggtt cctgcaccag attacccact ctggaccgcg gcggtttcgc
      841 tttccagcgg taaagcggtg cattatcttt gcgatgaatc ctctgactgg ttcccggacc
      901 tcgatgatat tcgcgctaaa attacgcctc gtacgcgtgg gatcgttatt atcaacccaa
      961 ataacccaac cggcgcggta tattccaaag agcttttaat ggagattgtg gagattgcac
     1021 gtcagcataa tctcattatc ttcgccgatg aaatttatga caaaattctc tacgacgatg
     1081 ctgagcatca ctcaattgcg ccactggcac ctgacctgct gaccattacc tttaacggac
     1141 tgtcgaaaac gtaccgcgtt gcaggcttcc gtcaggggtg gatggtgttg aacgggccga
     1201 aaaaacacgc caaaggctac atcgaaggtc tggaaatgct ggcttcaatg cgcctgtgtg
     1261 ctaacgttcc tgcgcaacac gccattcaga ccgcgctggg tggttatcag agcatcagtg
     1321 aatttattac ccctggcggt cgtctttatg agcagcgtaa ccgcgcgtgg gaactgatca
     1381 acgatattcc gggcgtttcc tgcgtgaaac ctcgtggtgc gctgtatatg ttcccgaaaa
     1441 tcgacgccaa acgctttaac attcacgacg atcagaaaat ggtgctggat ttcctgttgc
     1501 aggaaaaagt tctgttggtg caagggacgg cattcaactg gccgtggccg gatcacttcc
     1561 gcattgtcac gctaccgcgt gtcgatgata tcgagctgtc tttgagcaag ttcgcgcgtt
     1621 tcctttctgg ttatcatcag ctgtaatctt aatttcactg ccggagattg catccggcag
     1681 cgttatcccg ccacaatgac ctgatgatgt catcatacgt aaggtcacta tgaaacagag
     1741 ccattttttt gcccatctct cccgcctgaa actcattaac cgctggccgc taatgcgcaa
     1801 cgtgcggacg gaaaatgtgt ccgaacacag tttgcaggta gcgatggtcg cccatgcgct
     1861 ggcagctatc aaaaatcgaa aatttggcgg taatgtcaac gccgaacgta tcgctttact
     1921 ggcgatgtac cacgatgcct cagaagtgct caccggcgat ctccctactc cggtgaaata
     1981 cttcaattcg caaatcgctc aggagtacaa ggctattgaa aaaatcgctc agcaaaaact
     2041 ggtcaatatg gttccggaag agctgcggga tatctttgcg ccgttaattg acgagcatgc
     2101 atatagcgat gaagaaaaat cgctggtgaa acaggcagat gcactgtgtg catatctgaa
     2161 atgtctggaa gaactcgcgg ccggaaataa tgaattcttg ctggcaaaaa cgcgactgga
     2221 agcgacgctt gaagcgcgtc gcagccagga gatggactac ttcatggaag tatttgttcc
     2281 cagcttccat ctttcgctcg atgagattag ccaggattca ccgctgtaag cagccggagt
     2341 ctgcgtcgca tcaggcaata agcgccggat gcgacatcag gctcttgtca aaacggaaac
     2401 agcatcggga tcatcaccac acaaaccgcc atcacgataa tggtgaacgg tacccccaac
     2461 ttcacaaagt cactgaagct gtaatttccc ggacctaaaa ccagtgtgtt aacaggtgaa
     2521 gaaaccggtg tcataaaggc ggcggatgct gccatcgcca cgaccatcgc gaatggataa
     2581 ggcgacaccc ccatcgtttt ggcagcagcc agcgcaatcg gagccatcaa caccgccgtc
     2641 gcggtattag agataaatag cccaataacc gccgacaaga caaacaaaca ccccagcatc
     2701 atatgtggcc cgtaaccacc gccaatgtcc atcagccctt tcaccgccag cgcgacacct
     2761 cccgttttct gtaatgccac agcaaacggc atcatcccaa cgatcaaaat aatgctcggc
     2821 cagtgaatgg atttataggc gctttcagca tctatacagc ggaatttccc catcagcagg
     2881 caggcgatga tagcggcaac aggattagga atttcatctg tcagcattaa cgccaccatt
     2941 agcaccagac agaaaatggc atggggtgcc tggctgtgcg cgggcgatgc ttcactcacc
     3001 tcttccggta agttcagcgc tacgaagtcg cggccctgtt tggccagcat accgatcagt
     3061 ttccagttac ccacaaccag gatgatatcg cccagcagca gaggctcatc cgccagcgaa
     3121 ccttccagcg ccacgccatt gcgctttagc cccaccacat tcagtccgta gcgggtacga
     3181 aaaccaattt cgcgcaccga tttaccaatc agttctgact caggaattaa tgaaatctct
     3241 gccatgccca catcaagggc ctggtcagaa aaatactcgc cgcgcagtac catcggctcc
     3301 agcaattgct cactacaaaa ttgccggaga tcgacatcag ccgcagacat atcaataagc
     3361 aaaacgtcac gcgcgcgaaa ttcagaaacc ccattaacgt tcacgataac gcgacgaaaa
     3421 cgccgccagc gttcaacacc gatgacgtta gcgccataac gctcacgtaa tttgagatca
     3481 tccagccgtt gaccaatcat tggcgatccg gggcgaatag ccagacgtcg cgcacgcccg
     3541 gtcagtcgat attcacggat aagatcgcga aaggttcgac gcgtccagcc ttcgcgctgc
     3601 ggggtctggg tatccccttt cagcatgaaa cgcatcacta acatatacaa aatacccagc
     3661 accagcacga ccaggccaat aggtgttacg ctaaagaaac tgaagccgtg atagccttca
     3721 cgcagcaatt cactgttgac taccaggttc ggcggcgtcg ccaccagcgt catcatgccg
     3781 cttatcagcc cggcaaaact caacggcatc atcagacgtg acggcgaagt ttgcatacgc
     3841 atggcaacgc ttaacaccac gggaataaag atagcgacaa cgccggttga actcataaac
     3901 gcgccaagcc ccgcgacggt cagcatcaac aaaaccaaca ttttgatttc actgttgccc
     3961 gcaactttga ccagccatgt tcccattacg gtggcaacac cggtacggac caaaccatcg
     4021 ccaataataa acaaggcggc aatcaggaca acgttaggat cagaaaagcc ggaaaatact
     4081 tcggggactg tcagcgttcc gcttaatgca aacgcgacaa taacaaacaa agcgaccgca
     4141 tccatacgca ctctgcccgt cgcaaacaag acaatggcaa cggccagtaa tgaaagaacc
     4201 caaatcaatt caccgttcac aacttatcct tgttaattga gggagatgtc ttgattctgc
     4261 cataaaaaag ccccgacgag acggggctaa atcatgatca ggtgtttcac tgaataataa
     4321 catcgccatt tggctgtttg gtcacagtaa tttgctccag actgtggagg accaaatcga
     4381 cctcattcag gcgcggggta tctgccggag cgttaaccgc aatgacatga caacccgccg
     4441 ccaggccaga aagcacgcca gcggagcatc ttccaccacc acacactcct gcggcgcaag
     4501 ccccagcagc tgcgcgccta acagatacgc atcaggttct ggttttccgc gcttcactcg
     4561 ctcagcggtt acaaacacct ctggtgcggg aagcccagct attttatggc gcgctcgcgc
     4621 taccggcatg gagccagaag tcacaatggc ccacggaata cctgctttat tcaaatgact
     4681 gagtaaggcg attgcccccg gaagcgggta ataccttcgg tttccgtggc ctcgatgtgc
     4741 tccagacgcg taaactcggc ggcaatatca gcctcggatt tgcccgccat aaaatggcgc
     4801 agagaggtga tcgcctgttt accgtgaatg aaagccagca cctcttccgg cgctaaccca
     4861 tgacgtctgg cccagttgct ccacgcccgt tctaccgcag gcagggaatc caccagcgtt
     4921 ccatcaagat caaacagaaa acctttgcac cgcacgcggg cctcctcagg cattgataat
     4981 ttgattaatt tcgttggcgc tcaaatggta ctgacgcggg caggcatgcc acacattaag
     5041 catgcgctga tatttttccc acattggcgt ctgggcgtta aaaccgtgag ttccggcgtc
     5101 aaaatgggta tagcgccctt ccacattaac cataaagcgg acataaccga ggtaacgtgc
     5161 ttcagtggcg gcgtcaaagc cgaggaaggt gacacgacgt tcatcgatgg attgctggtc
     5221 ctgcaaatta gaccaggaaa catgcaacgc atgatacatc tccataatgt cgatgatagt
     5281 gcggcaggtt tcttctttca gctcgccaaa ctcgcgatcc aattcacgca tctgtaatcc
     5341 gtaacctcgc tcaataattg tttgcaggcg acggtaacgt tcagcatttg ccggatcgag
     5401 catagtcatc atcttgtact gattagacaa aataagacgt tgcgcgttgg tcatttccat
     5461 tgttgactcc tgtatcactc tactacggtg aaaaaaaaga aggctgagta tgccttcttt
     5521 tatatgcgta atcaggggtc aattacaaat catcaaggaa agttttatcc agttgtttga
     5581 aggcgcgctt aagcgtgtca gctaatgcct ggtaatcagg cttgccttca acgggtgcca
     5641 acacctgtcc agactcctgc aatttaccgc gaacttcata aaaccagtta aggattgcag
     5701 ggggtaatgg cgttacagaa cgcttgccca gccaccacaa tccctgcatg ggtaaactta
     5761 aggcgaacag tgcagtggca actgccggcc caagctgacc gcccagggca atctgccagc
     5821 agagagtaaa tacggcgatc ggcggcataa aacggatcgc ataacgcgtc atcttgataa
     5881 cgcgattttc gacaaagacc ggggcaaggc gtttttccag tggccacgtc tttgagtaat
     5941 gctgtccgcg gcgaaacaag ctaaaaaaat taacagaacg attatccggc gttgacatgc
     6001 ttcacctcaa cttcacatat aaagattcaa aaatttgtgc aaattcacaa ctcagcggga
     6061 caacgttcaa aacattttgt cttccatacc cactatcagg tatcctttag cagcctgaag
     6121 gcctaagtag tacatattca ttgagtcgtc aaattcatat acattatgcc attggctgaa
     6181 aattacgcaa aatggcatag actcaagata tttcttccat catgcaaaaa aaatttgcag
     6241 tgcatgatgt taatcataaa tgtcggtgtc atcatgcgct acgctctatg gctccctgac
     6301 gtttttttag ccacgtatca attataggta cttccatgtc gagtaagtta gtactggttc
     6361 tgaactgcgg tagttcttca ctgaaatttg ccatcatcga tgcagtaaat ggtgaagagt
     6421 acctttctgg tttagccgaa tgtttccacc tgcctgaagc acgtatcaaa tggaaaatgg
     6481 acggcaataa acaggaagcg gctttaggtg caggcgccgc tcacagcgaa gcgctcaact
     6541 ttatcgttaa tactattctg gcacaaaaac cagaactgtc tgcgcagctg actgctatcg
     6601 gtcaccgtat cgtacacggc ggcgaaaagt ataccagctc cgtagtgatc gatgagtctg
     6661 ttattcaggg tatcaaagat gcagcttctt ttgcaccgct gcacaacccg gctcacctga
     6721 tcggtatcga agaagctctg aaatctttcc cacagctgaa agacaaaaac gttgctgtat
     6781 ttgacaccgc gttccaccag actatgccgg aagagtctta cctctacgcc ctgccttaca
     6841 acctgtacaa agagcacggc atccgtcgtt acggcgcgca cggcaccagc cacttctatg
     6901 taacccagga agcggcaaaa atgctgaaca aaccggtaga agaactgaac atcatcacct
     6961 gccacctggg caacggtggt tccgtttctg ctatccgcaa cggtaaatgc gttgacacct
     7021 ctatgggcct gaccccgctg gaaggtctgg tcatgggtac ccgttctggt gatatcgatc
     7081 cggcgatcat cttccacctg cacgacaccc tgggcatgag cgttgacgca atcaacaaac
     7141 tgctgaccaa agagtctggc ctgctgggtc tgaccgaagt gaccagcgac tgccgctatg
     7201 ttgaagacaa ctacgcgacg aaagaagacg cgaagcgcgc aatggacgtt tactgccacc
     7261 gcctggcgaa atacatcggt gcctacactg cgctgatgga tggtcgtctg gacgctgttg
     7321 tattcactgg tggtatcggt gaaaatgccg cgatggttcg tgaactgtct ctgggcaaac
     7381 tgggcgtgct gggctttgaa gttgatcatg aacgcaacct ggctgcacgt ttcggcaaat
     7441 ctggtttcat caacaaagaa ggtacccgtc ctgcggtggt tatcccaacc aacgaagaac
     7501 tggttatcgc gcaagacgcg agccgcctga ctgcctgatt tcacaccgcc agctcagctg
     7561 gcggtgctgt tttgtaaccc gccaaatcgg cggtaacgaa agaggataaa ccgtgtcccg
     7621 tattattatg ctgatcccta ccggaaccag cgtcggtctg accagcgtca gccttggcgt
     7681 gatccgtgca atggaacgca aaggcgttcg tctgagcgtt ttcaaaccta tcgctcagcc
     7741 gcgtaccggt ggcgatgcgc ccgatcagac tacgactatc gtgcgtgcga actcttccac
     7801 cacgacggcc gctgaaccgc tgaaaatgag ctacgttgaa ggtctgcttt ccagcaatca
     7861 gaaagatgtg ctgatggaag agatcgtcgc aaactaccac gctaacacca aagacgctga
     7921 agtcgttctg gttgaaggtc tggtcccgac acgtaagcac cagtttgccc agtctctgaa
     7981 ctacgaaatc gctaaaacgc tgaatgcgga aatcgtcttc gttatgtctc agggcactga
     8041 caccccggaa cagctgaaag agcgtatcga actgacccgc aacagcttcg gcggtgccaa
     8101 aaacaccaac atcaccggcg ttatcgttaa caaactgaac gcaccggttg atgaacaggg
     8161 tcgtactcgc ccggatctgt ccgagatttt cgacgactct tccaaagcta aagtaaacaa
     8221 tgttgatccg gcgaagctgc aagaatccag ccctctgccg gttctcggcg ctgtgccgtg
     8281 gagctttgac ctgatcgcga ctcgtgcgat cgatatggct cgccacctga atgcgaccat
     8341 catcaacgaa ggcgacatca atactcgccg cgttaaatcc gtcactttct gcgcacgcag
     8401 cattccgcac atgctggagc acttccgtgc cggttctctg ctggtgactt ccgcagaccg
     8461 tcctgacgtg ctggtggccg cttgcctggc agccatgaac ggcgtagaaa tcggtgccct
     8521 gctgctgact ggcggctacg aaatggacgc gcgcatttct aaactgtgcg aacgtgcttt
     8581 cgctaccggc ctgccggtat ttatggtgaa caccaacacc tggcagacct ctctgagcct
     8641 gcagagcttc aacctggaag ttccggttga cgatcacgaa cgtatcgaga aagttcagga
     8701 atacgttgct aactacatca acgctgactg gatcgaatct ctgactgcca cttctgagcg
     8761 cagccgtcgt ctgtctccgc ctgcgttccg ttatcagctg actgaacttg cgcgcaaagc
     8821 gggcaaacgt atcgtactgc cggaaggtga cgaaccgcgt accgttaaag cagccgctat
     8881 ctgtgctgaa cgtggtatcg caacttgcgt actgctgggt aatccggcag agatcaaccg
     8941 tgttgcagcg tctcagggtg tagaactggg tgcagggatt gaaatcgttg atccagaagt
     9001 ggttcgcgaa agctatgttg gtcgtctggt cgaactgcgt aagaacaaag gcatgaccga
     9061 aaccgttgcc cgcgaacagc tggaagacaa cgtggtgctc ggtacgctga tgctggaaca
     9121 ggatgaagtt gatggtctgg tttccggtgc tgttcacact accgcaaaca ccatccgtcc
     9181 gccgctgcag ctgatcaaaa ctgcaccggg cagctccctg gtatcttccg tgttcttcat
     9241 gctgctgccg gaacaggttt acgtttacgg tgactgtgcg atcaacccgg atccgaccgc
     9301 tgaacagctg gcagaaatcg cgattcagtc cgctgattcc gctgcggcct tcggtatcga
     9361 accgcgcgtt gctatgctct cctactccac cggtacttct ggtgcaggta gcgacgtaga
     9421 aaaagttcgc gaagcaactc gtctggcgca ggaaaaacgt cctgacctga tgatcgacgg
     9481 tccgctgcag tacgacgctg cggtaatggc tgacgttgcg aaatccaaag cgccgaactc
     9541 tccggttgca ggtcgcgcta ccgtgtttat cttcccggat ctgaacaccg gtaacaccac
     9601 ctacaaagcg gtacagcgtt ctgccgacct gatctccatc gggccgatgc tgcagggtat
     9661 gcgcaagccg gttaacgacc tgtcccgtgg cgcactggtt gacgatatcg tctacaccat
     9721 cgcgctgact gcgattcagt ctgcacagca gcagtaacct cgtcatcatc cgcagctttg
     9781 cgctgcggat atctgaaccg gaaataatca ctatttccgg ttttttattc tcttaatctg
     9841 cattaatcct ttctgattat cttgcttaac tgcgctgcat caatgaattg cgccatttca
     9901 ctttacatac ttaccacttt gttttgtgca agggaatatt tgcgctatgt ccgcaatcac
     9961 tgaatccaaa ccaacaagaa gatgggcaat gcccgatacg ttggtgatta tcttttttgt
    10021 tgctatttta accagccttg ccacctgggt agttccggtg gggatgtttg acagtcagga
    10081 agtgcagtat caggttgatg gtcaaacaaa aacacgcaaa gtcgtagatc cacactcatt
    10141 tcgcattctg actaacgaag caggcgaacc tgagtatcac cgcgtacagc tgttcacgac
    10201 gggcgatgaa cgcccgggcc tgatgaactt cccgtttgaa ggattaacct caggatcgaa
    10261 atacgggaca gctgttggca tcatcatgtt tatgctggtg attggcggcg cgtttggcat
    10321 tgtgatgcgt acaggaacca ttgataacgg tatcctggcg cttattcgcc atacccgcgg
    10381 gaatgaaatt ctctttattc ctgcgctgtt tattctgttt tcacttggcg gcgcggtatt
    10441 tggtatggga gaagaggccg tcgcctttgc cattatcatc gcaccgctaa tggtccggct
    10501 gggctatgac agtattacca ccgtcctggt gacctatatt gccacgcaaa tcggttttgc
    10561 cagttcgtgg atgaacccgt tttgtgtggt cgttgctcag gggattgccg gcgttccggt
    10621 gctttctggc tccgggttgc gcatcgtggt gtgggttatc gccactatga ttggcctgat
    10681 ctttaccatg gtgtacgcct cacgagtgaa aaagaatcct cttctgtcac gcgtgcatga
    10741 gtccgaccgc ttctttcgtg aaaagcaggc ggatgttgaa caacgtccgt ttacctttgg
    10801 tgactggctg gtattgattg tcctgaccgc cgtaatggtc tgggtgattt ggggcgtgat
    10861 cgttaatgcc tggtttattc cagaaattgc cagccagttc ttcaccatgg gtctggtgat
    10921 tggcatcatc ggcgttgttt tccgccttaa cggcatgacg gttaatacca tggcttcatc
    10981 ctttaccgaa gggacgcgaa tgatgatcgc ccctgccctg ctggtgggtt tcgccaaagg
    11041 gattttgctg ctggtcggta atggtgaagc gggtgatgcc agcgtgttaa ataccatcct
    11101 caacagcatt gccaatgcca ttagcggtct ggacaacgcg gtcgcggcct ggtttatgtt
    11161 gctcttccag gcggtattta atttcttcgt gacgtccggt tctggtcagg cggcgttaac
    11221 catgccgtta ctggcaccgc ttggcgatct ggtcggtgtt aaccgtcagg ttaccgtgct
    11281 ggcattccag tttggtgatg gcttcagtca catcatttac ccaacctcag cttcgttaat
    11341 ggcaactctc ggtgtgtgca gggtggactt ccgtaactgg ctgaaggtgg gcgcgaccct
    11401 acttggactg ctgtttatta tgtccagcgt cgtagtgatc ggcgctcagt tgatgggcta
    11461 ccactaaaaa tgttaagagc cgcaattgcg gctctttttt cattctgccg tttcagtctc
    11521 tacggcttca tttttggcat tgcgcttcat ccacaacgcc agcgctttca gcgagtctgg
    11581 agtgaactca tcgcagcgtg cggtgatttc ttccggcgtc agccagcaaa cttcactgac
    11641 ttcatcttcc tgtagggcga agggaccgtg agagacgcag ctgaacaacg cgccccagac
    11701 acggcaattt ttatcttcga aatagaactg cccgtgctcg gcaaagggga caccggcaat
    11761 gcccaactct tcttccgctt cgcgacgcgc ggattccagc agttgctcat cggcctgaac
    11821 cacaccgcct gcggtcgcat ctaacatgcc gggtaaaaag tcttttgtct cggtacgacg
    11881 ctggaccaga attttgccca tgccatcatg cacgacgatg taagttgcac gatgacgcag
    11941 acactgtgcc cgcatttgtt cccggctggc ttgtgcaatg acttcgttct cttcattgac
    12001 aatatccacc cattcagtac ttgccaaacg acgctgttcc attatcagga aaccttcttt
    12061 ttctggcgct cttacggcgc attttgagtt gtgggtaaat tacggattaa tcgcgacctg
    12121 cgcaatgata ctttgatcat tgagtgcgat tacactaagt acgtcattat ccagcatgcc
    12181 ataactcgcc ggattaccgc ctttcggaat actcaccgag ccggggttga agtggaaaat
    12241 ctccccccgc tgttctgcca ctggcagatg ggtatgaccg tacaccagca catcgttctg
    12301 gtttaaagca ggtagatttt ccgggccaaa aagatgacca tgcgtcaaaa acagacgttg
    12361 tttttccagt aatacctgtt gccacggcgc ggttatcggg aaatgcagca gcatttgatc
    12421 cacttcgctg tcgcagttgc cgcgcacagc gataacctta tgtgccactt cattaagccg
    12481 ttcagcgact ttggctggcg cgtaaccctc cggtaaagca ttacgcgggc cgtgattcaa
    12541 cacgtcgcca aggatcacca gccactgggc accgctttgg gcaaacaact ccagaacacg
    12601 ttccgtcgcc ggtaacgacc catgaatgtc cgatgcaaac atcagtttca tcactcactc
    12661 ctcgtcgaaa aaacgtaagc tatgatactg gattctgtcg ttgctatcag ccagattgct
    12721 tcgccgaaag tgcaataaaa cgctggacag acgctcgctg ccactggaat gtcgcataat
    12781 ccaccaggcg ttctggcacc tcatcgccat gtagtaccag gcggttaatc attagcgcca
    12841 gatcagtatc agcaatgcac cattcaccaa ataaattcgg ctgacccagt gctaacaaat
    12901 gttctgccat cgcgaacagt ttctctgcac tggcttttcc ctcggccgtt agtggcgctt
    12961 ttttcgcccc cgcaaagaca acatccgtcg gacgctcttc gcggatgggc atcagatcgc
    13021 tgcgcagcca ggcctgaatc cgtcgtgcac gcgcacgatt ttctaaatca agcggataaa
    13081 tacgttccca ggtcggtggc gcaaatcgat cttccagata ctccgcaatg gcagaagatt
    13141 cactcaactc aaaatcatcg atttgtaata acggcacacg gcgtgtctga ccgtaacctt
    13201 gccacgtcgg cttcaaatgt tcaccgctgt cgaggtcgat ggtcttgata tgaaacgaca
    13261 ggcctttttc ctgcaacgcc acccaggcgg ataacacata aggggagaaa aagtgggcat
    13321 ctgaccaaag cgtgatagcg ggtttactca ttatgtcctc ggcaataaag ggggctggct
    13381 atcaaactat agagcctttt tgtcactgtc acgtgacgaa tactcacgta agtcgccgcg
    13441 ctttctatac ttgtggtgtt cagatgatta aacaaccgga gctgcaatga tcgatctcta
    13501 tttcgccccg acacccaatg gtcacaaaat tacgctgttt ctcgaagaag cagagcttga
    13561 ttatcgcttg attaaggtag acctggggaa aggcggtcag tttcgtccgg aatttttgcg
    13621 catttcgccc aacaacaaaa ttccggcaat tgttgatcat tctcctgccg atggcggcga
    13681 accgctaagc ctctttgagt ctggtgccat tttgttgtat ctgactgaga aaacaggact
    13741 ctttttgagt catgaaacgc gtgagcgcgc cgccacatta cagtggttat tctggcaggt
    13801 aggcggactg gggccgatgc ttgggcaaaa tcatcatttt aatcacgcag ccccccaaac
    13861 cattccttac gctattgaac gttatcaggt tgaaactcag cgtctttacc atgtactaaa
    13921 caagcggctg gaaaactcgc cctggctggg aggcgagaac tacagcattg cggatattgc
    13981 ctgctggccg tgggttaatg cctggactcg ccagcgaatt gacctcgcaa tgtatccggc
    14041 agtcaagaac tggcatgagc ggatccgttc gcgccctgcc accgggcagg cactgctaaa
    14101 agcacaactc ggtgatgagc gttcggatag ttaacagaaa caggttctcg tgtattattt
    14161 catcctaagt aaaacaacgg agaacctgca atggcacaac ctgccgctat tattcgtata
    14221 aagaaccttc gtttacgtac gtttatcgga attaaggaag aagaaattaa caaccgtcag
    14281 gatattgtta tcaatgtgac gatccactac cccgccgata aagcgcgcac tagcgaagat
    14341 atcaacgatg cgctgaatta tcgcaccgta acgaaaaaca ttattcagca tgtagagaat
    14401 aaccgtttct ctttgttgga aaaattaact caggatgtgc tcgatatcgc acgtgaacat
    14461 cactgggtga cgtatgctga agtggagatc gataaactgc acgcgctgcg ctacgccgat
    14521 tcggtatcca tgaccttaag ctggcagcgt taaccgccat atcgggaggc tgcatgaaca
    14581 tagtgatcac cggagggacg ggattaattg gtcgccattt gattccgcgt ttgctggagc
    14641 tgggccatca aatcacggta gtgacgcgta acccacagaa agccagttcc gttctcggcc
    14701 ctcgggtgac gctatggcaa gggctagccg atcaaagcaa cctcaacggc gttgatgcgg
    14761 taatcaacct ggccggagaa ccgattgctg ataaacgctg gactcacgag caaaaagagc
    14821 gtctctgcca aagccgctgg aatatcacgc aaaaactggt cgatttgatt aatgccagcg
    14881 acacgccgcc gtcggtattc atttccggct cggcaacggg ctattatggc gacttaggtg
    14941 aagtggtggt taccgaagag gaaccgccgc ataacgaatt tactcataaa ctctgcgccc
    15001 gctgggaaga gattgcctgc cgggcgcaaa gtgacaaaac gcgagtgtgc ctgctgcgaa
    15061 ccggcgtggt actggcaccg gacggcggta ttctcggtaa aatgctgcca ccgtttcgtc
    15121 ttggcctggg cgggccgatt ggttccggtc ggcagtatct ggcctggatt catattgatg
    15181 atatggtcaa cggcattctc tggctgctgg ataacgacct gcgcgggcca tttaatatgg
    15241 tttcgcccta cccggtacgc aatgaacaat ttgcccatgc gctcggtcaa gcgctgcatc
    15301 gcccggccat tttgcgcgtc cccgcaaccg ccattcggct gttaatgggc gaatcttcaa
    15361 tactggtatt aggtggacaa cgcgcgctgc ctaaacggct ggaagaagcg ggttttgcgt
    15421 ttcgctggta cgatttagaa gaggcgctgg cggatgtcgt tcgctgatgt ggtttacagc
    15481 aaacatccgc cagttaactc ccggtgttac aggattagtg gctttgcgcg ataagatcgt
    15541 ctggtgaaag tcgggtcacc atcataacta actctctgtc taaacctcta tccagcatct
    15601 cctgagcaat acgcagggct tcttcgtgtt tgccctgcat tgcgccttct tcacgtaatc
    15661 tgtcagcaat ggtcatcagt ttctcctttt cttgtggtgc gcgttctgtt atctcaccaa
    15721 taaatgcacg gaaacgctgg gcatcccctg tttgtaatac gtaattaaac agggctttta
    15781 gctgtctgtc attagtgttc cctgtaacta gcagcgaaac aatttggtcg actaatccca
    15841 acagatcgcg ctgacgaata tgtttctgaa ttaactccaa cagcgccatt ttgcggtgtt
    15901 gcataatctc gtcatccggc accacggtaa tatccaccaa cggaaacgct gatgaatata
    15961 ttttgcgggc tatagcaggc tcggcaaatt catcaagcca gcagagtgaa taaggataag
    16021 gacttctgca accatgataa aacagcatcg ggatcaccaa tggaagctct ttatagcccg
    16081 catcaagatg gttttgcatt gccgcaatgg aataacgcat catgcgaaaa gccattaatt
    16141 cttccagctt actttggtgc tctatcacta cataaatata acccactccc tcctgcgttt
    16201 tcacagacca caagaggtcg gaataatatt gccgcaggtc ttcatcaata aaactgtttg
    16261 attctagttt aagcgtcgtt aaatcacaca gtttgcgcag cggcgcggga agatgaatat
    16321 caataaaatc ccgcgcggtg t
//
LOCUS       DAEEKQ010000084        16292 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.084, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000084 DAEEKQ010000000
VERSION     DAEEKQ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.084"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..577
                     /locus_tag="KLK57_002561"
     CDS             <1..577
                     /locus_tag="KLK57_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HBE0892897.1"
                     /translation="GRPNNPFEPRRNRYAQKYLTLALMGGAAFFVLKGCGDSSDVDND
                     GDGTFYATVQDCIDDGNNADICARGWNNAKTAFYADVPKNMTQQNCQSKYENSYYDNV
                     EQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASRPVWRNTSGDYSWRSGSGKKES
                     YSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            580..1743
                     /locus_tag="KLK57_002562"
     CDS             580..1743
                     /locus_tag="KLK57_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HBE0892898.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWHASDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIDSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            1827..3452
                     /locus_tag="KLK57_002563"
     CDS             1827..3452
                     /locus_tag="KLK57_002563"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HBE0892899.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQVLCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCL"
     gene            complement(3569..3844)
                     /gene="yjfN"
                     /locus_tag="KLK57_002564"
     CDS             complement(3569..3844)
                     /gene="yjfN"
                     /locus_tag="KLK57_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HBE0892900.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(3993..4322)
                     /gene="bsmA"
                     /locus_tag="KLK57_002565"
     CDS             complement(3993..4322)
                     /gene="bsmA"
                     /locus_tag="KLK57_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HBE0892901.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            4504..5253
                     /gene="yjfP"
                     /locus_tag="KLK57_002566"
     CDS             4504..5253
                     /gene="yjfP"
                     /locus_tag="KLK57_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HBE0892902.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIIAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(5250..6005)
                     /gene="ulaR"
                     /locus_tag="KLK57_002567"
     CDS             complement(5250..6005)
                     /gene="ulaR"
                     /locus_tag="KLK57_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HBE0892903.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ERGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(6113..7177)
                     /gene="ulaG"
                     /locus_tag="KLK57_002568"
     CDS             complement(6113..7177)
                     /gene="ulaG"
                     /locus_tag="KLK57_002568"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HBE0892904.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            7532..8930
                     /gene="ulaA"
                     /locus_tag="KLK57_002569"
                     /pseudo
     CDS             7532..8930
                     /gene="ulaA"
                     /locus_tag="KLK57_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
     gene            8946..9251
                     /gene="ulaB"
                     /locus_tag="KLK57_002570"
     CDS             8946..9251
                     /gene="ulaB"
                     /locus_tag="KLK57_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HBE0892905.1"
                     /translation="MTVRILAVCGNGQGSSMIMRMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            9261..9725
                     /gene="ulaC"
                     /locus_tag="KLK57_002571"
     CDS             9261..9725
                     /gene="ulaC"
                     /locus_tag="KLK57_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HBE0892906.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            9739..10389
                     /gene="ulaD"
                     /locus_tag="KLK57_002572"
     CDS             9739..10389
                     /gene="ulaD"
                     /locus_tag="KLK57_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HBE0892907.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            10399..11253
                     /gene="ulaE"
                     /locus_tag="KLK57_002573"
     CDS             10399..11253
                     /gene="ulaE"
                     /locus_tag="KLK57_002573"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HBE0892908.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKARDWVKARMA
                     KAGTVEAA"
     gene            11253..11939
                     /gene="ulaF"
                     /locus_tag="KLK57_002574"
     CDS             11253..11939
                     /gene="ulaF"
                     /locus_tag="KLK57_002574"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HBE0892909.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATYLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGDAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            12036..12587
                     /locus_tag="KLK57_002575"
     CDS             12036..12587
                     /locus_tag="KLK57_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1440 domain-containing protein"
                     /protein_id="HBE0892910.1"
                     /translation="MAINGLFVRTDPQRRRYGVALLVGVLSGIISAFVKWGAEVPLPP
                     RTFSGGRDEFNPPYIFLRDYLGIDPTQTVYTFSEHIINPVMVTHIIFSLVFAIGYCIV
                     AEIFPKVKLWQGILAGLIVTVAVHGIFCPALNLTPPLTQLPFDEYASEILGHIFWFWI
                     IEIMRRDLRNRITHEPDPEVAIR"
     gene            complement(12662..12977)
                     /locus_tag="KLK57_002576"
                     /pseudo
     CDS             complement(12662..12977)
                     /locus_tag="KLK57_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
     gene            13304..13699
                     /gene="rpsF"
                     /locus_tag="KLK57_002577"
     CDS             13304..13699
                     /gene="rpsF"
                     /locus_tag="KLK57_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HBE0892911.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            13706..14020
                     /gene="priB"
                     /locus_tag="KLK57_002578"
     CDS             13706..14020
                     /gene="priB"
                     /locus_tag="KLK57_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HBE0892912.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            14025..14252
                     /gene="rpsR"
                     /locus_tag="KLK57_002579"
     CDS             14025..14252
                     /gene="rpsR"
                     /locus_tag="KLK57_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HBE0892913.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            14294..14743
                     /gene="rplI"
                     /locus_tag="KLK57_002580"
     CDS             14294..14743
                     /gene="rplI"
                     /locus_tag="KLK57_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HBE0892914.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(14855..15826)
                     /locus_tag="KLK57_002581"
     CDS             complement(14855..15826)
                     /locus_tag="KLK57_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HBE0892915.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKNKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(15879..>16292)
                     /locus_tag="KLK57_002582"
     CDS             complement(15879..>16292)
                     /locus_tag="KLK57_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HBE0892916.1"
                     /translation="WAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIGTFMDMT
                     PAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVSKLPINK
                     IIKPMLPFYAVMVLVLAMVTYIPQISMALPKALGY"
BASE COUNT         3825 a         3981 c         4333 g         4153 t
ORIGIN      
        1 tggtagaccg aataatcctt ttgaaccgcg tcgcaatcgc tacgcacaaa aatacttaac
       61 gttggcgcta atgggcggtg ccgctttttt cgtattgaaa ggttgtggcg atagtagcga
      121 tgtcgataac gacggcgacg gaacgtttta cgcgacagtg caggattgta ttgatgacgg
      181 taataatgcc gacatttgcg cgcgtggctg gaacaacgcc aaaacggcat tttatgccga
      241 tgttccgaag aatatgactc agcagaactg tcagtctaag tacgaaaatt cctactacga
      301 caatgttgaa cagagttgga tcccggtcgt ttctggattt ttgttaagcc gggttattcg
      361 taaagatcgc gatgagccgt tcgtctataa cagcggcgga tcctcatttg cttcccgccc
      421 cgtctggcgc aacacttccg gtgattactc ctggcgctcc ggttctggca aaaaagagtc
      481 ttactcttca ggcggcttta ccaccaaaaa agcgtcaacc gtttcgcgcg gcggctatgg
      541 tcgttcttcc agcgcccgtg ggcattgggg aggctaatca tgctgagaca caacgttcct
      601 gtgcggcggg atctggacca aattgccgct gacaacggtt tcgactttca tatcatcgac
      661 aatgaaatct attgggacga gagtcgggct taccgtttta ctctgcgcca gattgaagag
      721 cagatcgaaa aaccaactgc ggaactgcat cagatgtgcc ttgaggtggt ggatcgcgcg
      781 gttaaagacg aagagatcct gacgcaactg gcgatcccgc cgttgtactg ggatgtgatc
      841 gccgaaagct ggcacgccag cgatccttcg ctgtatggcc ggatggattt tgcctggtgt
      901 ggcaatgcgc cggtgaagct gctggagtac aacgccgata cgccgacttc gttgtatgag
      961 tcggcttatt tccagtggtt gtggctggag gatgcccggc gcagcggcat tattccgcgt
     1021 gatgccgatc agtacaatgc cattcaggaa cgcctgattt cacgctttag cgagctttat
     1081 agccgggagc cgttttattt ctgctgctgt caggacaccg atgaagacag gactaccgtg
     1141 ctgtacttgc aggactgcgc ccagcaggca gggcaggagt cgcggtttat ctacattgaa
     1201 gatctcggtt tgggcgtcgg cggtgtactg accgatcttg atgataatgt catccagcgt
     1261 gcatttaagc tgtatccgct ggagtggatg atgcgtgacg ataacggtcc gctgctgtgc
     1321 aagcgtcgcg agcaatgggt ggagccgtta tggaaaagta tcttgagtaa taaagggcta
     1381 atgccgctgc tttggcgctt cttccctggt catcctaacc ttcttgcatc ctggtttgag
     1441 ggtgaaaaat cgcagattgc cgctggcgaa agctatgtgc gtaaaccgat ctactcgcgc
     1501 gaaggcggaa acgtcaccat tttcgacggt cagaataacg tcgttgacca cgctgatggt
     1561 gattacgccg atgaaccgat gatctaccag gcgtttcaac ctctgccgcg atttggcgat
     1621 agctacacac tcatcgatag ctggattgtc gatgatgaag cgtgcggaat ggggatccgt
     1681 gaagataaca cactgatcac caaagacacc tcacgtttcg ttccgcatta cattgctgga
     1741 taagaatgtt ttagcaatct ctttctgtca tgaatccatg gcagtggcca tactaatggt
     1801 gactgccatt gatggaggga gacacagtgc actggcaaac tcacaccgtt tttaatcaac
     1861 ctataccatt aaataacagc aacttatacc tgtctgatgg cgcgctttgc gaagcggtaa
     1921 cgcgtgaagg tgctggctgg gatagcgatt ttctagccag tattggtcag cagttaggaa
     1981 cggctgaatc ccttgaactg gggcggctgg cgaatgtaaa cccgcctgaa ctgttgcgct
     2041 acgatgccca ggggcggcgt ctggacgatg ttcgtttcca tcccgcatgg cacctgctga
     2101 tgcaggtgtt atgtaccaat cgggtgcaca atcttgcctg ggaagaagac gctcgctccg
     2161 gcgcatttgt ggcgcgcgcg gcgcgtttta tgttacatgc gcaggttgag gctgggtcgt
     2221 tatgtccaat aacgatgacc tttgccgcca cgccattgtt gttacagatg ttacccacgc
     2281 cgtttcagga ctggaccacg ccgctgctga gcgatcgcta cgattctcac ttattgccag
     2341 gtgggcaaaa acgcggtttg ttgattggca tgggaatgac ggaaaagcag ggtggttccg
     2401 atgtcatgag caacaccacc cgtgcagagc gtttggaaga tggctcttat cggctggtgg
     2461 ggcataaatg gtttttctcg gttccgcaaa gcgatgcgca tctggtgctg gcgcagacca
     2521 cgggcggtct gtcctgtttt tttgtgccgc gctttttgcc tgacgggcaa cgcaacgcga
     2581 ttcgcctcga acggctgaaa gataagctgg gtaatcgctc taacgccagt tgtgaagtgg
     2641 agtttcagga tgccattggc tggttattgg ggcaggaagg ggaaggaatt cgtctgatcc
     2701 tgaaaatggg ggggatgacg cgttttgatt gcgccctggg tagccatgcc atgatgcgcc
     2761 gtgcgttttc gctggcgatt tatcatgcac atcaacgcca tgtttttggt aatccattga
     2821 tccaacagcc ccttatgcgt catgtcttaa gtcgcatggc acttcagctt gaagggcaaa
     2881 cggcgttgct gtttcgtctt gcgcgagcgt gggaccggcg cgccgatgcc aaagaagccc
     2941 tgtgggcgcg tttatttacg cctgcggcaa aatttgtgat ctgcaaacga ggtatgccgt
     3001 ttgtggccga agcgatggag gtgctgggcg gcattggtta ttgcgaggag agcgagctgc
     3061 cgcggcttta ccgggagatg ccggtaaaca gtatttggga aggttccggc aatattatgt
     3121 gcctggatgt gttgcgcgtt ctcaataagc aagcgggcgt atacgactta ttgtcggaag
     3181 catttgtgga agtgaaaggg caggatcgct attttgatcg cgcggttcgt cgtttacagc
     3241 agcagctgcg taagccagct gaagaactgg ggcgagagat tactcatcag ctattcctgc
     3301 tgggctgcgg tgcgcaaatg ttgaaatatg cttctccgcc aatggcgcag gcgtggtgtc
     3361 aggtgatgtt agatacgcgc ggcggcgtac ggttgtcaga gcagatccag aatgatttat
     3421 tgctgcgggc gacgggggga gtgtgtttgt aaacgtatac gactgatgcg acgctggttt
     3481 cgattaactc aatgaaatat gtgaaaattg taggccggac aaggcgctcg cgccgcatcc
     3541 ggcattgttc atttgctgta gatgacactc atgcatacag tatcgcctgt acgcgccagt
     3601 tatcaggctg ctggtcttcg tacatggtaa taatgcggta ccatgacgca ccttgttcat
     3661 cggcctttaa cgccacgata cgttccacgt cctgcggatc ccctgaaata ttgctaactg
     3721 agatctgacc tatttcattc aggcccgtca cgcagtcagc actggcgaat tctgcagact
     3781 gtgccgtggc gctcgcagga taagtcacta attgcagcga gggtgaggca agtaattgtt
     3841 tcatcgtcag ctccattttt cgtttcccca catgttgcag ggcattccat cgcggtaata
     3901 aacgcccagg cttccagccg gtaatttatt gtgcacaggt aaacgcatag ggacgcaaag
     3961 caatgtaaag ctgactatgt aagttgtctg atttatttac ggtacaaaat ggcttgtgaa
     4021 taccactgtc ctgtcacgat ggtttcgtcc accatgacga caacgtaata atcagctttt
     4081 gcagcgacag cttttgctct gatttctgct aatgcgtcat ccggagaacc ccgaaccatc
     4141 gtgcttacgc tacctattcg ctgtaaccct tgcgtctggt cgcggcgaat ctcttgcgga
     4201 tggtccgtta ctggcggtgc tggctgtggc gtaccttgca gtgcgctaca ggcacttaac
     4261 atcaacacca ataataaact ggcaaaccgg taaataacgc tattacgttt cctgctaacc
     4321 atagtgtagt gccttattaa tttaactttg ggggaatgtt cccgaattgt tacctggtac
     4381 gcgattttcg aatgaaaacg tgacgaaagc gtaacccaca tctcaacatt atgaactaat
     4441 accgccagca gaaacttgtg ctaaggattt cttcatgtca taccagacaa aaaggagaga
     4501 cagatgattg aaatagaatc acgcgagctg gcagatattc ccgttcttca tgcttatcct
     4561 gtcgggcaaa aagatacccc gttaccgtgc gtaatttttt atcacggctt tacttcatcc
     4621 agcctggtgt atagctattt tgccgttgcg ctggcgcagg ctggtttgcg ggtgatcatg
     4681 ccggatgcgc ccgatcacgg tagccgtttt agtggcgacg aagcgcggcg gttaaatcaa
     4741 ttctggcaaa tcttgctaca aagtatgcag gaattcacta ctttacgtgc ggcaatagcc
     4801 gaagaaaact ggctgcttga tgaccgtctg gcagtcggtg gcgcgtcgat gggcgcgatg
     4861 acggcactgg gaattaccgc tcgccacccc acggtgaaat gtaccgccag catgatggga
     4921 tcgggctatt ttacatcgct cgcccgttca ctgtttccac cgctgatacc tgaaacggca
     4981 gcacagcaga atgaattcaa taacattatc gcgccgctgg cagagtggga agcgacaaac
     5041 cacctggaac aacttgggga cagacctcta ctgctgtggc atggcctcga cgatgacgtt
     5101 gtgcctgccg acgaatcact acgtttgcag caggccttaa gcgagacggg acgggataaa
     5161 ctgctaacct gttcatggca gccaggcgtg cgtcaccgca ttacgcctga ggcgttagat
     5221 gctgccgtga catttttccg ccagcatctt taaacacgca gaatgctgac cccttgcgct
     5281 tccagttgtt gcaggatttc cgggttagca tttttgccgg tgataagcat atcgatttga
     5341 tcggcacggc taaaaagcat tcccgcgcgt tcgccaatct tactgctatc aaccagtacc
     5401 accagtttcc ctaccacgct cagcatcttc tgctctgcca ttgctgtcag catatcggtt
     5461 ttatacaacc cttctgcggt cagccctttt ccgctggtaa acatccagtg cccggcatag
     5521 agactgtttt cgctgccctg cgggcttaaa gtgatggact gacttttgtt gtactgtcca
     5581 cccataatga tcacgctgtc atgttcttga tcgatcaggt aatttgccag cggtagataa
     5641 ttagtgatga tttgcactgg cttgccacac atttcccgcc caagcagaaa cgcggtggag
     5701 ccgcagttga tgactacgct ttcgcccgga ttaaccagct gcgaggccgc tttagcaata
     5761 cgtacttttt catcgtgatt ctgcgcctga tgcagattca tcggcgtcca gcgcgggcgc
     5821 tgttgggtaa tagcttctgc gccattgcgc acctttttca gtttgccgcg ttcgtcaagt
     5881 ttattgatat cgcgtcgcgc agtggcaggc gaaattccca gacgctcaac gactttctca
     5941 acggtcacaa agcccaattg tgcgagcatt tccaggagga tttgatgtct ttgtgcttca
     6001 gtcatgagct attccgataa aatttgattt ttttagatga tatttgaaat agctaggaaa
     6061 tactacgccg gatagcgcga acttctccac gctatccggt ataaacgaca gattacagga
     6121 atgacttgaa cggcagatcc ggttcaatag tgaagcaatc atcgaaaccg cgcggatagt
     6181 ggtactcgaa gttgtcttta tccagcggcc aggtaaattt gccgccaacc tgccagataa
     6241 acggcttgaa gccatacttc agacgatctt ttttcatctc ccacagcacg cggatctctt
     6301 gcggatcggc ctggaagttt gaccagatgt cgtggtggaa cgggataacc actttcgcat
     6361 tcagggcttc acccatacgc agcatatcgg cgctggtcat tttgtcggta ataccgcgcg
     6421 ggttctcacc atacgatcct aacgccacat cgatctgatg ttcgttaccg tgcttcgcat
     6481 aatagttaga gtagtgggaa tcgccactgt gatataggga accgccaggg gttttgaaca
     6541 ggtagtttac tgcgcgatcg tccatgccat ctggcagtac gccagccgct ttttgatcgg
     6601 caggcagggt gatcagtgca gtacggtcga aagcgtcaag cgcatgaatt tcaatgtctt
     6661 tcacttttac aacatcgccc ggtttgacca cgatgcaacg ctctttcggt acgccccagc
     6721 caatccacaa atcaacgcag gttttcggtc caatgaatgg tacgtcatca gcacagttct
     6781 gcatcacggc agcagcgacg ttaacgtcga tatgatcgtt gtgatcgtga gtcgccagta
     6841 ccgcgtcgat ctggcgaatc gcaaacggat caagaacaaa cggggtggtg cgtaggtttg
     6901 gctgcagttt tttcacgcca gccatgcgct gcatctggtg accctgtttc attaacgggt
     6961 taccgtgact ttgtttgcca gtgccgcacc agaaatcaac gcaaacgttg gtgccacctt
     7021 ctgatttcaa ccagatcccg gtgcagccta gccaccacat cgcaaatgtg ccaggggcga
     7081 cctgctcttg ttcaatttct tcattcagcc agctacccca ctccgggaaa gtgctcagga
     7141 tccaggattc tcgggtgata cttttcactt tactcatcgc catttacctt catgatagtt
     7201 caattcgaat caatatgtga ttggttttga ttaatcctga cactattttt tcaggaaggc
     7261 aatgaccatt ttttgacttt ttgccaggga agttgttgtt gattttgagt gtggaaagat
     7321 ttaatggaat gtgtagttaa attaactaaa tgaatttaaa taaataattg tttcgttgtg
     7381 tgaatcccac tctatccgtg tggaattatt tgcgggtcgc gtcacattta atcataaata
     7441 atcttgttgt gattactttt gaaaattaga gtgagtgcac aacattccgg gtgtgcggaa
     7501 tacccggtta cctcttcttc aggagatcgt tatggagatc ctctacaaca tctttaccgt
     7561 gttttttaac caggtcatga ccaatgcccc gttgttgctg ggtattgtga cctgtctggg
     7621 ctacatccta ctgcgcaaaa gtgtcagcgt tattattaaa ggcacgatta aaaccataat
     7681 tggtttcatg ttgttgcagg cagggtccgg catcctcacc agcaccttca aaccggtggt
     7741 ggcgaaaatg tccgaagtct acggcattaa acggcgcaat ttccgatact tacgcgtcga
     7801 tgatggcaac catcgaccgc atgggcgatg cctatagctg ggtaggttac gcggtgttgt
     7861 tagcgctggc gctgaacatc tgttacgtgc tgctgcgtcg cattaccggt attcgtacca
     7921 tcatgctgac tggtcatatc atgttccagc aggcggggct gattgccgtt acgttgttta
     7981 ttttcggcta ctccatgtgg acaaccatta tctgcacggc gattctggtt tcgctctact
     8041 ggggcattac ctccaacatg atgtacaagc cgactcagga agtgacggac ggctgcggtt
     8101 tctccatcgg tcaccagcaa cagtttgcat catggactgc ctataaagtt gcgccgttcc
     8161 tcggcaaaaa agaggaaagc gttgaagacc tcaaactgcc aggctggctg aatattttcc
     8221 acgacaacat cgtctccacg gcgattgtca tgaccatctt ctttggtgcc attctgctct
     8281 ccttcggtat cgacaccgtg caggcgatgg caggcaaagt gcactggacg gtctatatcc
     8341 tgcaaaccgg tttttccttc gcggtggcga tcttcatcat cactcagggt gtgcgcatgt
     8401 ttgtggcgga actctctgaa gcgtttaacg gtatctccca gcgcctgatc cctggtgcgg
     8461 ttctggcgat tgactgtgcg gctatttata gcttcgcgcc gaacgccgtg gtctggggct
     8521 ttatgtgggg caccatcggt cagctgattg cggttggcat cctggtcgcc tgcggttcct
     8581 cgagcctgat tatcccaggc tttatcccga tgttcttctc caacgccacc atcggcgtgt
     8641 tcgctaacca cttcggcggc tggcgtgcgg cgctgaagat ttgtctggtg atggggatga
     8701 tcgaaatctt tggttgcgtc tgggcggtga aacttaccgg tatgagtgcc tggatgggga
     8761 tggcggactg gtcgattctg gcaccgccga tgatgcaggg cttcttctcc atcggtatcg
     8821 cctttatggc cgtcatcatt gtaattgcac tggcttatat gttcttcgct ggccgcgcac
     8881 tgcgcgcaga agaagatgca gaaaaacaac tggcagaaca gtctgcttaa taaggagttt
     8941 tgattatgac cgtacgtatt ctggctgtgt gtggcaacgg acaaggcagt tccatgatca
     9001 tgaggatgaa agtggaccag tttttaaccc aatcaaacat tgaccacacg gtaaatagct
     9061 gcgcggttgg cgaatacaaa agcgagttga gcggcgcgga tatcatcatc gcttctacgc
     9121 acattgcggg cgaaatcacc gtgaccggca acaaatacgt ggttggcgtg cgcaacatgc
     9181 tctctcctgc cgactttggc ccgaaactgc tggaagtgat caaagcgcat ttcccgcagg
     9241 atgtgaagta aggacgcgcc atgaaattac gtgattcgct ggcggagaat aaatccatcc
     9301 gcctgcaggc tgaagctgag acatggcagg acgcagtaaa aatcggcgtt gacctgctgg
     9361 tggcggcgga tgttgtcgag ccgcgttact accaggcgat tctggatgcg gttgaacagc
     9421 acggtccgta ctttgtgctc gcaccaggcc tggcaatgcc gcacggtcgc ccggaagagg
     9481 gcgttaagaa aaccggtttt gcgctggtga cgctgaaaaa gccgctggag ttcaaccacg
     9541 aagataacga cccggtggat atcctcatca ccatggcggc ggtcgatgcc aacacgcacc
     9601 aggaagtggg catcatgcag atcgtcaacc tgtttgaaga tgaagaaaat ttcgaccgtt
     9661 tacgtgcctg ccgtaccgag caggaagtac tggatctcat tgaccgcacc aacgcggcag
     9721 cttaagaagg aattgaagat gtcattaccg atgttgcaag tcgcgctgga caaccagact
     9781 atggatagcg cctacgaaac cactcgcctg attgccgaag aagtcgacat tatcgaagtg
     9841 ggtaccattc tgtgcgtggg cgaaggcgtg cgtgcggttc gcgatctgaa agcgctctac
     9901 ccgcacaaaa tcgtgctggc agacgccaaa attgccgatg cgggcaaaat tctttctcgt
     9961 atgtgcttcg aagccaacgc tgactgggtg accgtgattt gttgtgcgga tatcaacacc
    10021 gccaaaggtg cgctggacgt agcaaaagag tttaacggcg acgtgcagat cgaactgacc
    10081 ggttactgga cctgggaaca ggcgcaacag tggcgcgacg caggtattca gcaggtggtt
    10141 tatcaccgca gccgtgacgc gcaggccgct ggcgtggcgt ggggtgaagc cgatatcact
    10201 gcgatcaaac gtctttccga tatgggcttc aaagtcaccg tcaccggtgg cctggcgctg
    10261 gaagatctgc cgctgttcaa aggcattcca attcatgtgt ttatcgccgg tcgcagcatc
    10321 cgtgatgccg cttctccggt tgaagccgcg cgtcagttca aacgttccat cgccgaactg
    10381 tggggctaag gagcggatat gttgtccaaa caaatcccgc ttggcatcta tgaaaaagcg
    10441 ctccccgccg gggagtgctg gctggaacgc ctgcgactgg caaaaacgtt gggcttcgat
    10501 tttgtcgaga tgtcggtaga tgaaactgac gagcgcctgt cgcgcctcga ctggagccgc
    10561 gagcagcgtc tggcgctggt gaatgcgatt gttgaaaccg gcgtgcgtgt gccgtccatg
    10621 tgcctttctg cccatcgtcg tttcccgttg ggcagcgaag atgacgcggt gcgggcgcag
    10681 gggctggaga ttatgcgtaa agccatccag ttcgcccagg acgttggcat tcgcgtgatc
    10741 cagctggcgg gctatgacgt ttactatcag gaagccaata acgaaacgcg tcgtcgtttc
    10801 cgtgacggcc tgaaagagag cgttgagatg gcgagccgcg cgcaggtgac gctggcgatg
    10861 gagatcatgg attatccgtt gatgaactcc atcagcaagg cgctgggtta cgcgcactat
    10921 ctcaacaacc cgtggttcca gctctacccg gatatcggca acctgtcggc gtgggacaac
    10981 gatgtgcaga tggagttgca ggccggaatc gggcatatcg tcgcggtgca tgtgaaagac
    11041 accaaaccgg gcgtcttcaa aaacgtgcca tttggcgaag gtgtggtgga tttcgaacgt
    11101 tgcttcgaaa cgctcaaaca gagtggctat tgcgggccgt acctgattga gatgtggagc
    11161 gaaacggcgg aagacccggc ggcagaagtg gtgaaagcgc gtgattgggt gaaagcgcgc
    11221 atggccaaag caggcacggt ggaggcggca taatgcaaaa gctaaaacag caggtgtttg
    11281 aagccaacat ggatctgccg cgctatgggc tggtgacctt tacctggggc aacgtcagcg
    11341 ctatcgaccg cgaacgcggg ctggtggtga tcaagcccag cggcgttgcc tacgaaacca
    11401 tgaaagcaga cgatatggtg gtggtcgata tgagcggcaa ggtggtggaa ggggagtatc
    11461 gcccgtcttc cgacaccgcg acgtatctcg aactctaccg tcgttacccg tcgcttggcg
    11521 gcattgtcca tacccactcc actcatgcta ctgcatgggc gcaggcgggg ctggcgatcc
    11581 cggcgttagg cactacgcac gccgactact tctttggcga catcccgtgt acgcgcggat
    11641 taagcgaaga agaggtgcag ggcgagtatg aactgaacac cggcaaagtg attatcgaaa
    11701 cgctgggtga cgccgagccg ctgcatacgc cggggattgt ggtgtatcag cacggtccgt
    11761 tcgcctgggg gaaagatgcc cacgacgcgg tgcataacgc ggtggtgatg gaagaagtgg
    11821 cgaaaatggc gtggattgcg cgcagtatta acccacaact gaatcacatc gacagcttcc
    11881 tcatgaataa acacttcatg cgtaagcacg gtcctaacgc ctattacggg cagaagtaag
    11941 cagtgcgccc cggaattatt acctcctgcg ggatgtaatt ggtctgattc cggggccgct
    12001 tcagttaaaa aatcgtataa acaacaggtg tttttatggc tatcaatgga ttgtttgtca
    12061 gaacggatcc gcaacgtcgc cgctatggcg ttgcgttact tgtgggtgta ctttcaggaa
    12121 ttatttcagc atttgttaaa tggggagcgg aagtcccctt gccaccgcgc acattttccg
    12181 gtggtcgcga tgaatttaac ccgccgtata tctttctacg ggattattta ggtatcgacc
    12241 caacccaaac tgtgtataca ttctcggaac atattatcaa cccggtgatg gtgacgcata
    12301 tcatcttctc actggttttt gcgatagggt attgtattgt cgcggaaatt ttcccgaaag
    12361 tgaaattatg gcaggggata ttagcagggc ttattgtgac agtggcagtc cacggtatat
    12421 tttgcccggc gctgaattta acgccgccgc tcactcaatt gccttttgac gaatatgcct
    12481 ctgaaatcct ggggcatatt ttctggttct ggataataga aattatgcgc cgcgacttac
    12541 gtaatcgcat tactcatgag ccagatccgg aagtcgcaat ccggtgaata accgcataaa
    12601 aaagccccct gcgaaggggg caaagcaaac tatggcaatg tttcgttggt tatacctggt
    12661 gctagcgata aatatccgcg ctggcgtgca tattgccgtt actccccggt tcccgcatca
    12721 gaattacgtg gtagtacgtt actccccggt tcccgcatca gaattacgtg gtagtacgtt
    12781 gcgccctgcg catctgtctc ttcatttaat gcctgacgcg cttcgctttc agtggcgaaa
    12841 ttatgattga tataaatcac gcctaagctt tgcacatcat ccatgtttct ggcctggtgg
    12901 ttattaattt caatggctgc ccatgtattt gcacttagca aaagcacagc cagaagggct
    12961 aaaactcgac tgaacataga tacctcctcg acggctgact ttgtgtgctc tcctctgtga
    13021 tgatcttctg atttaatttt aatcaatgat aaagaagttg atggtgacca tttctgatgc
    13081 agttgttcaa aaaaacacca tgatgaagtg tgatgaactc caaatcagcg gtttagaggt
    13141 taattgcgaa aggggagatt tatttcggct ctgcccttga gtttagcggg gcatacaagt
    13201 actataacgc gtcatttttc agccgacctt taacacgttc cttgcctccc cgggattcgg
    13261 ctgacccaga caggaggctg aataatccgt aaggagcaat tcgatgcgtc attacgaaat
    13321 cgtttttatg gtccatcctg accagagcga acaggttccg ggcatgatcg agcgctacac
    13381 tgctgccatc actggtgcag aaggcaagat ccaccgtctg gaagactggg gccgccgtca
    13441 gctggcttac ccgatcaaca aactgcacaa agcacactac gttctgatga acgttgaagc
    13501 tccgcaggaa gtgatcgatg agctggaaac taccttccgc ttcaacgatg ccgttatccg
    13561 cagcatggtt atgcgtacca agcacgctgt taccgaagca tctccgatgg ttaaagcgaa
    13621 agacgagcgc cgtgagcgtc gcgatgattt cgcaaacgaa accgctgatg atgctgaagc
    13681 tggggattct gaagagtaat ttctgatgac caaccgtctg gtgttgtccg gcaccgtgtg
    13741 caggactccc cttcgaaagg tcagtccatc aggaattcct cactgccagt tcgtgcttga
    13801 gcatcgttct gtgcaggagg aagccggttt tcaccggcag gcgtggtgcc aaatgcccgt
    13861 tattgttagc ggacacgaaa accaggccat tactcacagt ataacggtcg gcagtcgcat
    13921 aaccgttcag gggttcattt catgccacaa ggcaaagaac ggactgagca aaatggtttt
    13981 gcatgccgag cagattgaat tgatagattc tggagactag ccatatggca cgttatttcc
    14041 gtcgtcgcaa gttctgccgt ttcaccgcgg aaggcgttca agagatcgac tataaagata
    14101 tcgctacgct gaaaaactac atcaccgaaa gcggtaagat tgtcccaagc cgtatcaccg
    14161 gtacccgtgc aaaataccag cgtcagctgg ctcgcgctat caaacgcgct cgctacctgt
    14221 ccctgctgcc gtacactgat cgccatcagt aatcggtcac ggtccattaa tacgactttg
    14281 agaggataag gtaatgcaag ttattctgct tgataaagta gcaaacctgg gtagcctggg
    14341 tgatcaggta aacgttaaag cgggctatgc tcgtaacttc ctggtaccgc agggtaaagc
    14401 tgttccagct accaagaaaa acattgaatt cttcgaagca cgtcgcgctg aactggaagc
    14461 taaactggct gaagttctgg cagctgctaa tgctcgcgct gagaaaatca atgcactgga
    14521 aactgttacc atcgcgtcta aagctggcga cgaaggtaaa ctgttcggtt ccatcggtac
    14581 tcgcgacatc gctgacgctg taactgcagc tggcgttgaa gtggctaaga gcgaagttcg
    14641 tctgccgaac ggcgttctgc gtaccactgg cgagcacgaa gtgagcttcc aggttcacag
    14701 cgaagtattc gcgaaagtga tcgtaaacgt agtagctgaa taattcgtta ttcaacgaga
    14761 cgtaaaaacg ccgaccagtg gtcggcgttt tgcttttgta gcgagcactt gcagatatcg
    14821 ataagggcca caataagcag ccctacgaag ataattactt agctgcctga atatcagcca
    14881 gcattggggc aagggctggg ttgtccttag cgaagtttgc gtacattggc tgtactttct
    14941 cgataaacgg agctttatct acctggtaga ctttcacgcc cattttatcc agttttgcaa
    15001 attcttgttt ctcttcctcg ctccacaagt ctttcatgta gctcattgat tctttagctg
    15061 cttttaatat aactgcctga ttttccgggc taagtttatc aaacgcagca ttgctcatca
    15121 gaaccacatc gggaaccata gtgtgttcgt caaggctgaa ggattttgcc acttcaccgt
    15181 gacgcatggt ggtcagcgcc atcacgctgt tttctgccat atcgaccaca ccctgctgta
    15241 aggctgtata caactcgccg taatccagtg gcgtcgggtt accgcccatg actttgatca
    15301 tttcaactgc gccagggctt ggctggacac ggactttcat gcctttcaga tcgtctggtt
    15361 tcagtactgg tttattgcca tagaaactgc gggcgcctcc atcataaaaa gtcagcccga
    15421 aataaccttt gcttttcgtt gagtcgagga tttttctccc gatatcgccc tgcatgacct
    15481 gataataatg atcacgatcg cggaacaaat aaggcaggct aaataattta taggaatttt
    15541 caaatgcttc catttctgcc gcattggttt ttaccagtgg aatagcgcca gaacgaatca
    15601 gctccattgt ttctcgttga gtacccaatg ttccatttgg ataaatacga atagtaatat
    15661 caccgttaga gtactctttg ttctttttcg caaagaactc catcgcttta tgaacaggat
    15721 gagacttatc ctgattatga ctcagtttta atgtcactgc ccaggactgg cttgcggata
    15781 acataagaac agcaccgata atgccagcgg atacttttat tttcatagat gttccttttc
    15841 taataaatat ggcggcaggt gccgccagtg aatgcgcatc agtagcccag tgcttttggc
    15901 aatgccatac tgatttgcgg aatataggtc accattgcca gcaccaatac catcacggca
    15961 tagaatggca gcataggttt gataatttta ttaatcggta atttagatac actacaaccg
    16021 acaaataaca cactgcctac aggcggagta caaataccga tacacatgtt cagtaccatg
    16081 acaataccaa agtgaatcgg gtccatgccc agttcggtga caacaggtaa aaatatcggc
    16141 gtaaaaatca gtaccgccgg tgtcatatcc ataaaagtgc cgataatcaa caggataata
    16201 ttgataataa gcaggattat tattgggttg tcagaaatag tgttaagcaa atcgagaatc
    16261 aggaagggaa cgtcagcgtt tgacattgcc ca
//
LOCUS       DAEEKQ010000085        15961 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.085, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000085 DAEEKQ010000000
VERSION     DAEEKQ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15961
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.085"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..453)
                     /gene="spy"
                     /locus_tag="KLK57_002583"
     CDS             complement(<1..453)
                     /gene="spy"
                     /locus_tag="KLK57_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HBE0892917.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPA"
     gene            complement(783..1751)
                     /gene="astE"
                     /locus_tag="KLK57_002584"
     CDS             complement(783..1751)
                     /gene="astE"
                     /locus_tag="KLK57_002584"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HBE0892918.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGDPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(1744..3087)
                     /gene="astB"
                     /locus_tag="KLK57_002585"
     CDS             complement(1744..3087)
                     /gene="astB"
                     /locus_tag="KLK57_002585"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HBE0892919.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRLAAKQ
                     GLLKMKTLADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAPVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLREGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(3084..4562)
                     /gene="astD"
                     /locus_tag="KLK57_002586"
                     /pseudo
     CDS             complement(3084..4562)
                     /gene="astD"
                     /locus_tag="KLK57_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
     gene            complement(4559..5593)
                     /gene="astA"
                     /locus_tag="KLK57_002587"
     CDS             complement(4559..5593)
                     /gene="astA"
                     /locus_tag="KLK57_002587"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HBE0892920.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRVDFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(5590..6810)
                     /gene="astC"
                     /locus_tag="KLK57_002588"
     CDS             complement(5590..6810)
                     /gene="astC"
                     /locus_tag="KLK57_002588"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HBE0892921.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDLQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPPDIRHAAYNDINSASALIDDATCAVIVEPIQGEGGVVPASNAFLQGLRELCDRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLTTEECASVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKAGVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            7256..8062
                     /gene="xthA"
                     /locus_tag="KLK57_002589"
     CDS             7256..8062
                     /gene="xthA"
                     /locus_tag="KLK57_002589"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HBE0892922.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            8229..8939
                     /locus_tag="KLK57_002590"
     CDS             8229..8939
                     /locus_tag="KLK57_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBE0892923.1"
                     /translation="MNAERKFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFVLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            8944..9621
                     /gene="ydjY"
                     /locus_tag="KLK57_002591"
     CDS             8944..9621
                     /gene="ydjY"
                     /locus_tag="KLK57_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HBE0892924.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYATPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKRGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            9636..10343
                     /locus_tag="KLK57_002592"
     CDS             9636..10343
                     /locus_tag="KLK57_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HBE0892925.1"
                     /translation="MMKMQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAADLTSIRFRSFFIATGLGQLPTTIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            10343..10891
                     /locus_tag="KLK57_002593"
     CDS             10343..10891
                     /locus_tag="KLK57_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HBE0892926.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSSDKLLAVAD
                     WRQNPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            10901..12067
                     /locus_tag="KLK57_002594"
     CDS             10901..12067
                     /locus_tag="KLK57_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE0892927.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTQGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKEAPDDA
                     TFARVTAPLWQYLDALHPYLWREGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFREGMIGNVHFVTIPANANASAAAKVVANFLLSPDAQLRKADPVV
                     WGDPSVLDPQKLPDGQREILQSRMPQDLPPVLAEPHAGWVNALEQEWLRRYGTH"
     gene            12040..13576
                     /locus_tag="KLK57_002595"
                     /pseudo
     CDS             12040..13576
                     /locus_tag="KLK57_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            13576..14229
                     /locus_tag="KLK57_002596"
     CDS             13576..14229
                     /locus_tag="KLK57_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HBE0892928.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALL
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            14296..15603
                     /gene="ynjE"
                     /locus_tag="KLK57_002597"
     CDS             14296..15603
                     /gene="ynjE"
                     /locus_tag="KLK57_002597"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HBE0892929.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTLDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKLPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(15612..>15961)
                     /locus_tag="KLK57_002598"
     CDS             complement(15612..>15961)
                     /locus_tag="KLK57_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBE0892930.1"
                     /translation="FYALVPFGFILAAPEQNALAGGWLLFAFIGTGSSFLAFAALAAK
                     HQIDNPGYAHKSFYYLGGLTEGTETILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRV
                     WSGYLTLKSLQRQ"
BASE COUNT         3701 a         4345 c         4162 g         3753 t
ORIGIN      
        1 cgctggacgt tctgtcagac gcttctcaaa attagcatta aattgctttt tctgttccgg
       61 cgtcaggatg ttgtaaattt tgttctgggt ttccatgtgc gccagcatgt tagctttgcg
      121 ctgttcttcc atttttgcga tctgcgcttc agcttttgct ttatcgaagg tatcgctggc
      181 aatgatgtca tgcattgcgc ggcgttcttc cagcggcgga cgtttcatct ggtcacgctg
      241 gcctttcatg atttcgcgga tctgctgttt ctgcgcgtcg gtcaggttca ggtctttgaa
      301 catcatgtcc tgatgcggac cgaacttgcc tttgtggtgc atcatcggct tcgcgtcagc
      361 cggtgctgcg gtagtggtgt ctgcggcatg ggccaggtta gccgcgccaa gagccagggt
      421 agaggcaaca aacagtgcag ttaatttacg catattctat atccttcctt tcagttattt
      481 attacggctt tcttaagtag cgtgccgtgt tgacgagatt aactttactg actttagcgt
      541 caattaatca gagcaacggt aaaacaatga aagtgtaaaa aacacttttg cgccaattat
      601 ggagaaaaaa agaaaatttg atggagagtg atgagagaat attacaacac aatgattttg
      661 cagagattat gaagaactat accggatgac tggtgataaa taaagcaaat aaccaggatt
      721 aatctgtatt aatttataag aaagcaactt aatacccgca gaatgatttc tgcgggtaag
      781 tattagctta ttttttcgag cattaatccc gcgcgtaatc ccaacgctac caacggatta
      841 gggaataaca catactctac atcatgggtt acggtaaaac gttcctctcc gtcctgcgcc
      901 agcaatgttc ctttctcaaa cggcataaaa ttcagcgtgt cacttgccat atgcatttcg
      961 aaggacggcg agtggcgagt aatttgcgaa accacccgat aacggagcgg cggtgttctc
     1021 acgataccga cactctcacc agatagcagc gcagcaattg cgctggcagt tactgcaaac
     1081 tggcgaagat cgttttgccc aaagggcaac gctttgccaa gttccagcgt acaggccagc
     1141 gcgccaaaat gtctggcgct gaaatgggta aacgtaccac caggctcctg atggaacacc
     1201 agcgcctcca gccccgccgc acccagccac gtcagaaatt tctcgtccca gggaatgtcg
     1261 cgttgcggta atacaccgaa ctgaggatgc aaggagccac gaattgcggt atgtagatca
     1321 aggtgccaac gcacagattc tttgccctgg tcataaaaat cttccaggca ctgttccagt
     1381 tcgcgcgccc gacaggtttc tccgctttca gcaaatagct gccaacgacc gccaaacatt
     1441 cggttcatat cgctatggca ataacgtttc ccttgcttca gcgcaggagg atccccgagg
     1501 atcaccagca accgccaacg taacgggatc tcgccgtgag atatcgcgcc aagcaacgcg
     1561 tccagcattt ccacaggtgc cgtctcatta ccgtggatcc ccgcagaaat caccagcgcg
     1621 ccttgcggtg gcgttaatgg cgtcagttcc agcacaccat cgcccagcca gcgccagcga
     1681 acgccgttga tttctcgctc ggtgataacc ggttttttac ccgttaaggt cagagcaaga
     1741 aaattatcca ttgccccctc cctcgcgctg gaacggataa accgaaccga gattcagtaa
     1801 ttggctcaat acatccagtg cttcccgccc ttcgcgcagc aattgcgggt cggccagatc
     1861 ggcagcagta aggcgatcgc ggtagtaacg atccacccag tcattgagcg cattaaacag
     1921 cgtatcgttc atcatcaccg ccggattcac cgcccggcgt tcttcttctg tcaatactac
     1981 ccgcaaccgc aggcacgccg ggccgccgcc attcgccatg ctttcacgta aatcaaagac
     2041 ttttagttcg ctaatcgggt tgtcagcggc aaggagttca ttgagataac cccatactcc
     2101 ggcgtgttcc cgacactcct gaggcagcac caacatcatg gaaccatcat cgcggctcaa
     2161 cagttggctg ttaaacagat aggtagacac cgcatccgac acggaaacct gagttgccgg
     2221 aacttctatc gccataaaac cgttcacccg cgcccgcagg tttgccagta actgtgactg
     2281 gcgagcgaac gcctgttggt ggcaaaacag cacctggcgg ttacttacgg caatcacgtc
     2341 attatgaaaa acaccctggt cgataacgtc cgggttttgc tgggcgaaaa tcacctgttg
     2401 gggattcacc tgattaagcc ttgccaccgc ctcgctggct tcgcgagtct gtcgcgccgg
     2461 ataacgggaa ggccgggtat cattgccttc ttctcgcccg tagacaaaaa gttgcatacc
     2521 cggctcaccg taatgaccgc cgagacgatt gtggtttgcc gccccctcat caccgagcaa
     2581 cgctacctgc ggcaacgccg aatggacgct aaatttctct tcgtcgttaa aaatagcttt
     2641 taacagcgat tcagtgacgg gcgcttccag cgaacggtga aatttattgt tcaggttggc
     2701 aacggtgaga tgcactttac catccagcgt atcggcagat ggcgcgatcg ttgccgcatt
     2761 ggctacccac attggcgaag cggagctgac gctggaaagc cagtgcggtg cctggcgtgc
     2821 aactttttcc agtacctgct catcgctacc actgaatccc aactgacgca gcaccggaat
     2881 aaacggacgc tcgtgcggcg ggatcacggc ctgggggaat cccgcatcgg caagggtttt
     2941 cattttcagt aagccctgtt tcgccgccag tcgcgggtta gacacctgaa aacggtgacg
     3001 ggtagaggct tcattaccaa acgacaggcc agcgtaatga tgcgtcagcc ctaccagccc
     3061 gtcgaaatta acttcccagg cgttcatcgc accacctcat cggaaaaatc cagcccgggg
     3121 ttaagcgttg cgggcaaagt taacgagtcc gactccaggc tcgccatagg ccatgcgcag
     3181 taatctgcgg cataccaggc gctggggcga tggttaccgg atgcaccaat gccgccgaat
     3241 ggcgcggtac tggcagcacc agtaagcggt ttgttccagt taacaatccc cacccgcgcc
     3301 tccagcaaca gttgatcgaa tttttcccgc tcgggggaaa ccagaccgca agagagtccg
     3361 aagcgagtgt tattcgccat tagaatcgct tcctcgaaag aatcataacg ccagacgcgc
     3421 aataacggtc cgaacacctc ttcatctggt acgccagcaa cgcctgtcat ttcaatgatc
     3481 cccggcgtca gcaacgatgt ctctgattgt aataagcgcg gcgcaagcag ggttcgtccg
     3541 cccatcgctt ccagttgctg ctaggcagta accacctgct gtgcggcctg ttcagaaatc
     3601 agcccgccaa taaacggctg cggttcgtca tcccagttgc ccggcgttaa tcgctggctg
     3661 acggcaacca gacgagcaag aaacgcatcg ccctgtgctc cgcttttgag caataaacgg
     3721 cgggcgcagg tgcagcgttg cccggctgtg acaaacgccg actgaatggt cagatggaca
     3781 gccgagtcga tatccgccac ctcatcgata attagcggat tattaccgcc catctcaagg
     3841 gcgagaattt tctccggctg accggagagc tggcgatgca gctggtagcc ggtattggcg
     3901 ctaccggtaa acagcaaacc gtcgagatcc tccagcgcac tcagcgcctg acccgtttca
     3961 cgcccgccct gcaccaggtt cagcacgccc ggcggcaagc cagcctgctg ccataaacgc
     4021 attaccgctt cgccactcca cggtgtcagt tcgctgggtt taaagataat ggtgttacct
     4081 gccagcaatg ccggaacgat atgtccgttc ggcaaatgac cagggaaatt atacggccca
     4141 aacaccgcca gcacgccgtg cgggcgatgt cgcaggctcg ccgcaccgtc cggcatttca
     4201 ctacgctgct cgccggtacg aacgtgatac gccttaattg atatcgcgat tttattgatc
     4261 atcgccgtca cttcggttgc cgcttcccag cgcggcttac ccgtttctct ggcaataatc
     4321 gcggttaatt cggctttatt gctttccagc aatccggcaa agcgttcgac aacgacctga
     4381 cgttcagcca atgagagccg cgcccagcgc ggaaacgccg cacgggctgc ccgacaagcc
     4441 tgttcgacct gagcggcatc ggcatcattg ccttgccata acacctcgcc cgataccgga
     4501 ttacgcttca cacgcgatgc gccctggccc gttatccagt caccgttaat ccataaagtc
     4561 atgctgtttt ctcctctgcg cacaggcgca ccagacgaac gcgatcccct gcgtggcatt
     4621 tgagggcatc cagttgtgcg gcggttaaaa tcaaacgctc ggttgccgga tcggtacgcg
     4681 ccagcaccac gcggaaatgg tgataatttt cattggcgac caggcaggcc ggaaaatcgc
     4741 cctgcgcagg ctgcccttct gccacttcca ccagccgact tttacggatg gcgcgcacgc
     4801 ggtcgatgtc acactcaagc gttggcccgc cgtcgaagat gtcgatatag ttgcggtaac
     4861 gaaaaccttc tttctccagc accgcgcggg caggcgcggt ttgcggatgt acctgaccga
     4921 tgacgtcctg ggcttcctgg gataaaaagt gggtatagat cggatgtttc ggcatcagtt
     4981 ctgcaataaa tgccttttgc ccggtgccgc agagaaaatc gacgcggcta aaatccatcg
     5041 aaaagaagcg tttaccgagg ctttgccaga acggtgaata gccgtgttcg tcaatcaccc
     5101 cgcgcatttc agcaaccact ttgtcattaa acttgtcgcg aaaagccgcc ataaacataa
     5161 agcgcgattt cgacagcaaa tagccgttgc cctctttgcg ccagtccggg tcgagaaaca
     5221 gcgtgcacag ctcgctgctg ccggtgtgat cgttactaag aaacagcgtc ggcaatgcgt
     5281 tatagacatt cagctctttt gaggcgtgaa ccaacgtgcc gacccgatag ttgtaccagg
     5341 gatcgttcag cccaaccgcc acctcaatgg cgcaaatccc cgccacggtg cctgtctcgc
     5401 tatcttccag cacgaacaca tagccctgct cgcttttggg cagttcgcct tgccaggttt
     5461 tgattgccct ttcgatacgc gcggaaagcg tggcttcatt ggcgggaagc gacgtcaggc
     5521 cgccgcccgt tttgctggca agctgcatca gcgccgagac atctgagcgc tcaacgggac
     5581 ggatgaccat catgatgaac ctcggctaac aaagtgttcg caagcagctg caaagcgatc
     5641 cagtccggtc gtcacctctt cttcgctgac attgagcgca ggcgcaaaac gcaccacgtt
     5701 gccacccgca atcagtacca taacgcctgc tttcgccgcc tcctgagaga tctgtttcgc
     5761 ttgtccggcg taatcggcat tcagtacaca gccaatcaac aaacctaagc cgcgaacttc
     5821 actgaacaaa ccgtagcggt gattaatggt attaagacgc tcaacaaacc agtcgtgacg
     5881 ctgtttaacg ccattaagca tctctggtgt gttgatgagc tccagcactt tgcccgccac
     5941 cgccgaggcc agcggattgc cgccataggt ggtgccatga gtgccaacgg tcatcacgct
     6001 ggcgcactct tcggttgtta acaacgcacc gaccgggaaa ccgccgccca gcgctttggc
     6061 ggtagttaac agatcaggtg tcacgccgta gtgcatatag gcatacagtt ccccggtgcg
     6121 cccgacgccg gtttgtactt catcaaaaat caacagcgca ttgtggcggt cacacaattc
     6181 acgcagacct tgtaaaaacg cgttgctggc tggcaccaca ccgccttccc cctggatggg
     6241 ttcgacaatc accgcacagg tggcgtcgtc aatcagcgcg ctggcagagt taatatcgtt
     6301 atatgcagca tgacggatat ctggtggcag tggcgcaaaa tcctgtgaat aggctggctg
     6361 cccacccgca ctgacagtaa acagcgtgcg accatgaaac gcatttttaa acgccacgat
     6421 accgctctta tggctgccgt agcggtcgtg agcgaattta cgcgccagtt ttagcgccgc
     6481 ttcgttggct tccgcaccgg agttacaaaa gaagacgcga tcggcaaacg tggcgtcgat
     6541 caattttttc gccagtcgca gtaccggctc gttggtgtaa ccgttgccgg tatgccagaa
     6601 cttactcgcc tgttcgttca gcgcttcacg cagttccgga tgcgcatggc ccagcgcgtt
     6661 caccgcaatg ccacccgcga agtcgatata ctctttcccc tgcagatccc acaagcgcga
     6721 accttcgcca cgtaccggta taaagggtgc cggagcgtaa acaggtatca tccattcatc
     6781 aaagttttca cgcgtaattg gctgagacat agcgacctct acagtaaata attcgctatc
     6841 tatatgttaa caataagtaa tgtttgcgct gtaaatgtag attgcagggt tcgtgccagc
     6901 cagtgataaa agtgcataaa cggcggaggc taactggaaa tcaaggagtt ataaccaaac
     6961 catatgcatt taaagtgcat ataaagtgaa tacgtttgcg atgtgggtga ataaaaagaa
     7021 taaaaaacgc aatgttatgc agaagtaaaa tataattctg gaattgtgat cattgacgaa
     7081 atttactgga aattactgcg ccattctgac gcagcgcgca ccaaaagcgg gcattttttg
     7141 cgccatcgtt gacatcatta acaaccatcg atcaaatcac ttaacaacag gcggtaagca
     7201 acgcgaaatt ctgctaccat ccacgcactc tttatctgaa taaatggcag cgactatgaa
     7261 atttgtctct tttaatatca acggcctgcg cgccagacct caccagcttg aagccatcgt
     7321 cgaaaagcac caaccggatg tgattggcct gcaggagaca aaagttcatg acgatatgtt
     7381 tccgctcgaa gaggtggcga agctcggcta caacgtgttt tatcacgggc agaaaggcca
     7441 ttatggcgtg gcgctgctga ccaaagagac gccgattgcc gtgcgtcgcg gctttcccgg
     7501 tgacgacgaa gaggcgcagc ggcggattat tatggcggaa atcccctcac tgctgggtaa
     7561 tgtcaccgtg atcaacggtt acttcccgca gggtgaaagc cgcgaccatc cgataaaatt
     7621 cccggcaaaa gcgcagtttt atcagaatct gcaaaactac ctggaaaccg aactcaaacg
     7681 tgataatccg gtactgatta tgggcgatat gaatatcagc cctacagatc tggatatcgg
     7741 cattggcgaa gaaaaccgta agcgctggct gcgtaccggt aaatgctctt tcctgccgga
     7801 agagcgcgaa tggatggaca ggctgatgag ctgggggttg gtcgatacct tccgccatgc
     7861 taatccgcaa acagcagatc gtttctcatg gtttgattac cgctcaaaag gttttgacga
     7921 taaccgtggt ctgcgcatcg acctgctgct cgccagccaa ccgctggcag aatgttgcgt
     7981 agaaaccggc atcgactatg aaattcgcag catggaaaaa ccgtccgatc acgcccccgt
     8041 ctgggcgacc ttccgccgct aatttagcag ctctcctggc tcaaactggg tcaggagaat
     8101 taaccttgag aaaaatcaac aaactgtcag taatgatttg ttgcctgccg tcctttgtta
     8161 taccgtcact gcgtttttag ttgtctgacc acttctctat tatcaagttt gatataggaa
     8221 actccacgat gaatgctgag cgtaaatttc ttttcgcctg tcttattttt gcgctggtca
     8281 tttacgctat ccacgctttc ggtttattcg atctgctcac cgatttaccc cacttacaga
     8341 cactcattcg ccagagcgga ttgttcggtt atagcctcta tattctgtta ttcatcattg
     8401 ccaccctctt tgtgttacca ggaagcatac tggtgatcgc tggaggaata gtttttggcc
     8461 cgctcttagg gacactactc tcattaattg ccgccacgct ggcctcctcg tgctcattcc
     8521 tgctggcgcg gtggctggga cgtgatttac tgctgaaata cgtgggccat agccatacct
     8581 tccaggccat tgaaaaaggc attgcgcgta acggtattga ttttcttatt ctgacccgct
     8641 taatcccgtt gtttccttac aatattcaaa attacgctta cggattaacc accatcgcct
     8701 tctggcctta tacccttatt tcggcactca cgaccctgcc cggtattgtt atttataccg
     8761 tgatggcaag cgatctcgcc aatgaaggca ttacgctgcg ctttatttta caactctgtc
     8821 tggcgggcct ggcgctgttt attctcgtcc agctcgcaaa actctacgcc cgacacaaac
     8881 atgtggatct gtctgcttcg cgccgcagcc cgcttactca cccaaaaaat gaaggataga
     8941 acgatgtcgc aacattattc agtgtcatgg aaaaaaggac tggctgcact ctgcttactg
     9001 gctgttgccg ggcttagcgg ctgcgatcaa aaagagaatg cggcggcaaa agtggaatac
     9061 gacggacttt cgaacagcca accgctgcgc gtcgatgcca ataaccatac ggtaaccatg
     9121 ttggtgcaaa ttaatggtcg tttcctcacc gacgacactc gtcacggtat tgtgtttaaa
     9181 gatggctcca acggacataa atcgctgttt atggcttatg cgaccccgaa agcattttat
     9241 gaagccctga aagaggcagg tggtacgccg ggcgaaaaca tgacgatgga taataaagaa
     9301 acgactcatg tcacaggcag caaactggat atttcggtca actggcaagg ggcggcaaaa
     9361 gcgtattcct tcgatgaagt gattgttgac agtaatggca agaaactgga catgcgcttt
     9421 ggcggtaatt taacggcagc agaagagaag aaaacgggtt gcctggtgtg tctggatagc
     9481 tgcccggtcg gcatcgtcag caatgcaaca tacacttatg gtgcggtgga aaaacgcggc
     9541 gaagtgaaat tcaaaggcaa tgcctcagtt ctcccggcgg ataacacgct ggcaacggtt
     9601 acctttaaaa tcaccgaata aagccaggat aaaggatgat gaagatgcaa tcgcgtaaaa
     9661 tctggtacta ccgtataacc ctcatcatcc tgttgttcgc tatgctgctc gcatgggcgc
     9721 tgcttcccgg cgtccatgag tttatcaatc gcagcgttgc ggcgtttgcc gccgtggacc
     9781 aacagggtat agaacgcttt attcagtctt acggcgcact ggcggcagtt gtctcgttct
     9841 tgttgatgat tttacaggcc attgctgcac cgctacctgc gtttttgatc acctttgcca
     9901 atgcgtcgct gtttggcgcg ttctggggcg gcttgctgtc gtggaccagt tcgatggccg
     9961 gcgcggcgct gtgctttttt atcgccagag tgatgggccg cgaagtggtg gaaaaattaa
    10021 ccggcaaaac cgtgcttgac agtatggacg gctttttcac tcgctacggc aaacacacca
    10081 tcctggtctg tcggttattg ccttttgtcc ctttcgatcc aatcagctat gctgccgatc
    10141 tgacttcaat acgttttcgc tcgtttttta tcgccaccgg gcttggtcaa ttaccgacga
    10201 ctattgttta ttcctgggcg ggcagcatgt taacaggcgg tactttctgg tttgtcaccg
    10261 gactgtttat tctgtttgcc ctgaccgtgg tgatttttat ggcgaagaaa atatggcttg
    10321 agcgccagaa gaggaatgac tgatgggttt accgccgctt agcaaaattc ctttcatttt
    10381 acgtccgcag gcgtggctgc atcgtcgcca ttacggcgag gtgctaagcc cgattcgctg
    10441 gtgggggcgg atcccgttta tcttttattt ggtgtcaatg tttgttgggt ggctggagcg
    10501 caaacgctca ccgctcgatc cggtagtgcg atcgcttgtc agcgcgcgca ttgcgcaaat
    10561 gtgcctgtgt gagttttgtg tggatatcac cagtatgaaa gtcgccgagc gtaccggcag
    10621 cagcgataaa ctgctggcag tggctgactg gcggcaaaac ccgctcttta gcgatgaaga
    10681 acggctggcg ctggagtacg ccgaagccgc aagcgtcaca ccgccaacag tcgatgatgc
    10741 cctgcgtacc cgactggctg cgcattttga cgctcaggcg ctcaccgaac tgacggcatt
    10801 gatcggcctg caaaacctgt cggctcgttt taattctgcc atggacattc ccgctcaggg
    10861 gctgtgccgt attccggaaa aacgttctta aggagagaag atgcgccatt gtgggtggtt
    10921 gctgggattg ttatcgctgt tttctttggc aacacatgcc agtgactggc aggaaattaa
    10981 aaatgaggcc aaagggcaaa ccgtctggtt taacgcctgg ggcggcgata ccgcaattaa
    11041 ccgctatctc gactgggtta gcggcgagat gaaaacccat tacgctataa acctgaagat
    11101 tgttcgtctc gcggatgccg cagacgcggt gaagcgcatt cagaccgaag ctgctgccgg
    11161 tcgtaaaacg ggcggctcgg tggatctgct ctgggtgaac ggcgaaaact tccgcacctt
    11221 aaaagaggcc aatttattac aaacgggctg ggcggagact ctgcccaact ggcgctatgt
    11281 cgacacacag ctgccggtgc gggaagattt ttcagtgccg acacaaggtg cggaatcgcc
    11341 ctggggcggc gcacaactga cgtttatcgc ccgccgcgat gttacgccac agccaccaca
    11401 aacgccgcaa gccttactgg agtttgctaa agccaatccc ggcacggtta cttatccgcg
    11461 cccaccggac tttaccggca cggcgtttct tgaacagttg ctgattatgc tgacgccaga
    11521 tcccgccgca ttaaaagaag cgccggacga tgcgactttc gcccgtgtca ctgcgccctt
    11581 gtggcaatat cttgatgcac tgcatccgta tttgtggcgc gaaggaaagg atttcccgcc
    11641 ttcgcccgcg cggatggatg ctctgctgaa agccggcaca ttgcgcctgt cgctgacctt
    11701 taaccccgcg catgcgcagc aaaaaatcgc cagcggcgat ttgccggcaa gcagttacag
    11761 ttttggcttt cgcgagggga tgattggcaa cgtgcatttc gtcaccattc cagccaacgc
    11821 gaatgccagt gctgcggcga aggtagttgc caatttcctg ctctcacccg atgcgcaact
    11881 gcgtaaagca gatcccgttg tctggggcga tccttctgtt ctcgatccgc aaaaactgcc
    11941 tgacgggcag cgcgaaatat tgcaatcaag aatgccgcag gatctgccgc cggtactggc
    12001 tgaaccgcac gcaggatggg tgaatgcact ggaacaagaa tggttacgcc gttacggtac
    12061 gcattaatct ttttgctgtg ggcgatggtg gcggtgattt atgcaccgct gatcccggca
    12121 gctctcacgc tgatatcgcc tgcgttgtcg ttgacacact ggcaggcgtt atttgccgat
    12181 ccacagttac cgcatgcatt actggcaacg ctggtatcga caaccatcgc ggcggtcggg
    12241 gcattgttga ttgccctgct ggtgattgtg gcgctatagc ctgggccgaa atggcagcgt
    12301 atgtgcgccc gtctgccgtg gctgctcgcc attccccatg tggcttttgc caccagcgcc
    12361 ctgctgctct ttgctgacgg agggctgcta tatgactatt tcccgtattt cactccgcca
    12421 atggaccgat ttggcatcgg tctgggcctc acccttgcag tgaaagaaag cgcatttctg
    12481 ctgtggatct tagcggcagt attgagcgaa aaacggctgt tgcagcaggt cattgtgctg
    12541 gattcgctgg gctacagccg ctggcaatgc ctgaactggc tgctgttgcc ctccgtcgcg
    12601 ccttcgctgg caatggcgat gctggcgatt gttgcctggt cgctttcggt cgtggatgtg
    12661 gcgattattc ttgggccagg taatccaccg acgctggcag taattagctg gcagtggtta
    12721 actcagggcg acgcggatca acaaacgaaa ggcgcactcg ccagtctgct gctgatgctg
    12781 ttactcgccg cctacgtttt gctgagctat ctgctatggc gcagctggcg gcgcactatt
    12841 ccccgcgtag atggcattcg caatgcctgc cacaccttta ttaccaggca ctacgctggc
    12901 gagtttttta cccttaaccg gtgtgctgtg tgtggttctg ctggcgatcc tcgcggatca
    12961 gtcgacgatc aatagtgaag cactcatcaa cagtctgaca atggggctgg tagcaacatt
    13021 catcgctttg ctcctgttac tgctgtggct ggaatggggg ccacagcgtc gccagttgtg
    13081 gctatggttg cccattctat tgcccgatct gccactggtg gcgggccagt acacgctggc
    13141 gctatggctg aacctggatg gaagctggac ggcggtggtc tgggggcatc tgctgtgggt
    13201 aatgccgtgg atgctgttta tcctgcaacc cgcctggcag cgcattgatt cacggttaat
    13261 tttgattgcg caaacactgg gctggtcgcg ggccaaaatc ttcttttacg tgaaatgccc
    13321 actcatgttg cgccctgtgc tgattgcctt cgcggtggga ttttcagtca gtattgcgca
    13381 gtatatgccg acgctgtggc tgggcgcggg gcgttttccg acgctcacca ctgaggcggt
    13441 ggcattaagc agcggcggca gcaacggtat tctcgccgcc caggctttat ggcaactgtt
    13501 gttaccgctt attatttttg ccctgaccgc gttagtcgca aaatgggtag gttatgtcag
    13561 acaaggactc cgctaatgct ctgcgtgaaa aatgtttcgc tacgtttacc agaaagccgc
    13621 ttgctgacaa acgttaactt tacggttgat aaaggtgaca tcgtcacgtt aatggggccg
    13681 tctggctgtg gaaaatccac tctgttttca tggatgattg gtgcactggc tggacagttt
    13741 tcttgtacag gtgagctatg gctcaatgag caacggattg acatgctacc caccgcacag
    13801 cgtcagattg gcattctttt ccaggatgca ctgttatttg accagttcag cgtcgggcaa
    13861 aatttactgc tggcgctact gtcgacgctc aaggggactg ccaggcgcaa tgccgtgaaa
    13921 gacgcgcttg atcgagcggg actggctgaa acttatcacc aggaccctgc gacattatca
    13981 ggcggtcagc gagcgcgggt ggccctgctg cgcgcccttc tcgctcagcc aaaagcgtta
    14041 ctcctggatg agccattcag ccgtcttgat gtggctctgc gcgataattt tcgccagtgg
    14101 gtgttcagcg aagttcgcga actggcgatc cccgtcgttc aggtaacgca cgatctccag
    14161 gatgttcctg ctgatagttc tgttctggat atggcgcagt ggtcagaaaa ttacaacaaa
    14221 ctgcgataac gcaaagtttt tctcaatgcg tcagttcaga atggcgcact caaaactaca
    14281 atgtcgggat tttcgatgaa acgtgtttct caaatgaccg cgctggcaat ggctttaggg
    14341 ctggcttgcg cttcttcgtg ggccgctgaa ctggcgaagc ctcttacact tgaccagctt
    14401 caacaacaaa atggcaaagc gatagatact cgccccagcg cgttttataa cggctggcca
    14461 caaaccttaa atggcccttc tggtcatgaa cctgccgcct taaacctctc tgccagctgg
    14521 ctcgacaaaa tgagcaccga acagctcaac gcgtggatca agcaacataa cctgaaagcc
    14581 gatgccccgg tggcgctgta cggtaatgac aaagatgtcg acgccgtcaa aacgcgactg
    14641 caaaaagcag gttttacgca tatctccatt ctgagtgacg cgctaagcga accttcccgt
    14701 ctacaaaaac tgccgcactt tgagcagctg gtttatccgc aatggctgca cgacctgcaa
    14761 caaggtaaag aggttacggc gaaacctgcc ggtgactgga aagtcattga agcggcctgg
    14821 ggcgctccta agctttacct tatcagccat attcccggtg ctgactacat cgataccaac
    14881 gaagtggaaa gcgaaccgct gtggaacaaa gtttctgatg aacaactaaa agcgatgctg
    14941 gcaaaacacg gcattcgcca tgacaccacg gtcattctgt acgggcgtga cgtatacgct
    15001 ggagcgcgtg tggcgcaaat tatgctctac gcaggcgtga aagatgtgcg tctgctggat
    15061 ggcggctggc aaacctggtc cgacgcggga ctgcctgttg agcgaggaac gccaccgaaa
    15121 gtgaaagcgg aaccggattt cggcgtgaag ctcccggcac aaccgcagtt gatgctcgat
    15181 atggaacagg cccgtggact gctgcatcgc caggatgcat cgctggtgag cattcgttcg
    15241 tggccagaat ttatcggtac gaccagcggt tacagctata ttaaaccaaa aggtgaaata
    15301 gccggagcac gttggggaca cgctggtagc gactcgacgc atatggaaga ttttcataac
    15361 ccggatggca ccatgcgcag cgccgatgat attaccgcta tgtggaaagc gtggaatatc
    15421 aaaccagagc agcaagtttc attctactgc ggcaccggct ggcgcgcgtc cgaaaccttt
    15481 atgtacgcac gcgccatggg ttggaagaat gtttccgtgt atgacggcgg ctggtacgaa
    15541 tggagcagcg atccaaaaaa tccggtagca accggtgaac gcggcccgga cagtagcaaa
    15601 taacattgca attactgacg ctggagcgac ttcagcgtca gatacccact ccacacccgc
    15661 gtaaaggtcg tcatccagca caacgcaccg aagatccacg caaaccacgc aaaccatgca
    15721 gggaataaac atcccagcac aaacagtaag atcgtttctg tgccttcggt taatcccccc
    15781 agatagtaaa acgacttatg tgcataaccg gggttatcaa tctgatgttt tgctgccagt
    15841 gcagcaaagg cgagaaaact gctgcccgtg ccgataaacg caaacaacaa ccagccgcct
    15901 gccagcgcat tttgctccgg tgcagcaaga ataaagccaa acggcaccag cgcgtaaaag
    15961 a
//
LOCUS       DAEEKQ010000086        15883 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.086, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000086 DAEEKQ010000000
VERSION     DAEEKQ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15883
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.086"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            413..1027
                     /gene="evgA"
                     /locus_tag="KLK57_002599"
     CDS             413..1027
                     /gene="evgA"
                     /locus_tag="KLK57_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HBE0892931.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGILVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            1032..4625
                     /gene="evgS"
                     /locus_tag="KLK57_002600"
     CDS             1032..4625
                     /gene="evgS"
                     /locus_tag="KLK57_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HBE0892932.1"
                     /translation="MKFLPYIFLLCCGLWSTISFADEDYIEYRGISSNNRVILDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQRVRGINADYLNLLKRTLNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVASPPLNDDIAATKPLIITFPALVTTLHDSMRPLT
                     SSKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVVLNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDILPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIKVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDHAVYI
                     CGWQDITETRDLIHALEVERNKAINATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYSTGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALICSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4681..5826)
                     /gene="yfdE"
                     /locus_tag="KLK57_002601"
     CDS             complement(4681..5826)
                     /gene="yfdE"
                     /locus_tag="KLK57_002601"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HBE0892933.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5900..6844)
                     /gene="yfdV"
                     /locus_tag="KLK57_002602"
     CDS             complement(5900..6844)
                     /gene="yfdV"
                     /locus_tag="KLK57_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HBE0892934.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6914..8608)
                     /gene="oxc"
                     /locus_tag="KLK57_002603"
     CDS             complement(6914..8608)
                     /gene="oxc"
                     /locus_tag="KLK57_002603"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HBE0892935.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8662..9912)
                     /gene="frc"
                     /locus_tag="KLK57_002604"
     CDS             complement(8662..9912)
                     /gene="frc"
                     /locus_tag="KLK57_002604"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HBE0892936.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDSAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10425..11048)
                     /locus_tag="KLK57_002605"
     CDS             complement(10425..11048)
                     /locus_tag="KLK57_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HBE0892937.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            11345..11620
                     /gene="ypdI"
                     /locus_tag="KLK57_002606"
     CDS             11345..11620
                     /gene="ypdI"
                     /locus_tag="KLK57_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis lipoprotein YpdI"
                     /protein_id="HBE0892938.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(11697..11939)
                     /locus_tag="KLK57_002607"
     CDS             complement(11697..11939)
                     /locus_tag="KLK57_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HBE0892939.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            12292..13212
                     /gene="lpxP"
                     /locus_tag="KLK57_002608"
     CDS             12292..13212
                     /gene="lpxP"
                     /locus_tag="KLK57_002608"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HBE0892940.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNPKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            13568..13639
                     /gene="ypdK"
                     /locus_tag="KLK57_002609"
     CDS             13568..13639
                     /gene="ypdK"
                     /locus_tag="KLK57_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HBE0892941.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(13704..14942)
                     /gene="alaC"
                     /locus_tag="KLK57_002610"
     CDS             complement(13704..14942)
                     /gene="alaC"
                     /locus_tag="KLK57_002610"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HBE0892942.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDITEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            15319..>15883
                     /gene="ypdA"
                     /locus_tag="KLK57_002611"
     CDS             15319..>15883
                     /gene="ypdA"
                     /locus_tag="KLK57_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HBE0892943.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSDGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVSILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIV"
BASE COUNT         4481 a         3692 c         3370 g         4340 t
ORIGIN      
        1 tatctctcat ttcgcataga tgaaatttat gaattgaatg aaaagagtat tcattcttca
       61 tatcaaacac agtgcatgtg ttgcacatta tcccaggaac gttggaggcc taatttaaat
      121 gaggtgcaaa aaaaacaaca gaggactaaa ccgtggcttt tgcaatacaa ttcttacgcc
      181 tgtaggatta gtgagaagac ttatagtgct aacttgaaac tataaatcat cggtataatc
      241 cctgatttta ttgttgacat ttcatttatg ccgactattt atatggtata cttgtcgaat
      301 tatcttaaag gaagctcaga ttttcttatt tttattgaga aaatgagatg acgccttatg
      361 tctgtattac tacagggaga agggagatgc ttcattgcaa agggaataat ctatgaacgc
      421 aataattatt gatgaccatc ctcttgctat cgcagcaatt cgtaatttat tgatcaaaaa
      481 cgatattgaa atcttagcgg agttaactga aggcggaagc gccgttcagc gggtggaaac
      541 acttaagcct gatatcgtca tcattgatgt cgatatcccc ggagttaacg gtatcctggt
      601 gttagaaacg ctgaggaagc gccaatatag cggaattatt attatcgtct ccgctaaaaa
      661 tgaccatttt tacgggaaac attgtgctga tgctggcgct aatggtttcg tgagtaaaaa
      721 agaaggcatg aacaatatca ttgcggctat tgaagctgca aaaaatggct actgctattt
      781 ccccttctct ctcaaccggt ttgttggaag tttaacgtcc gaccagcaaa aactcgactc
      841 cttatcgaaa caagaaatta gtgtcatgcg gtatattctt gatggcaagg ataataatga
      901 cattgcggaa aaaatgttca tcagcaacaa aactgtcagc acttataaaa gtcgcctgat
      961 ggaaaaatta gaatgtaaat cactgatgga tctttacaca ttcgcacaac gtaacaaaat
     1021 cggctaacca catgaagttt ttaccctata tttttcttct ctgttgtggt ctttggtcga
     1081 ccataagttt cgcagacgaa gattacatcg aatatcgtgg catcagtagt aacaaccgtg
     1141 tcatacttga tccagtacgt ctgagcaaca aggaattacg ttggttagcg agcaaaaaaa
     1201 atcttgtgat tgcagtacat aagtcccaaa cggctacgtt gttgcatacc gattcgcagc
     1261 aacgagttcg tggtattaat gctgattatt taaatctttt aaaaagaacg ttaaatatca
     1321 aattaacact ccgggaatac gccgatcatc aaaaagcaat ggacgcgctg gaagaaggtg
     1381 aagtcgatat agtgttatca catttagtag cttcgccgcc tcttaatgat gacattgctg
     1441 caaccaaacc actgataatt acctttccgg cgctggtaac cacccttcac gattcaatgc
     1501 gaccgcttac ctcatcaaaa ccagtaaata ttgctcgagt agcaaattat ccccctgacg
     1561 aggtaattca tcaatcattt ccaaaagcaa caattatctc ttttacaaat ttatatcagg
     1621 cattagcatc cgtctcagcc ggacagaatg attactttat tggtagtaac atcattacca
     1681 gcagtatgat ttcccgctat ttcactcact ccttaaatgt agtgaaatat tataactcac
     1741 cgcgtcaata taattttttc ttgaccagaa aagaatctgt cgttcttaat gaagtactca
     1801 atcgatttgt tgatgctttg acaaatgaag ttcgctatga agtatcacaa aattggcttg
     1861 atacaggaaa cctggccttt ctgaacaaac cattagaact cactgaacat gaaaaacaat
     1921 ggattaagca gcatcccgat ttaaaggtgc tggaaaatcc ttactcccct ccctattcta
     1981 tgacggatga aaatggctcg gttcggggcg ttatggggga tattcttaat attattactt
     2041 tgcaaacagg tttaaatttt tctccgatca ccgtttcaca caatatccat gctggaacac
     2101 agctgaatcc cggcggatgg gatatattac ccggtgctat ttatagtgaa gatagagaaa
     2161 ataatgtttt atttgctgaa gccttcataa caacgcctta cgtttttgtc atgcaaaaag
     2221 cgcctgacag tgaacaaaca ttaaaaaaag gaatgaaagt tgccattcca tattattatg
     2281 agcttcattc gcaattaaaa gagatgtatc cggaggtaga gtggataaaa gtcgataacg
     2341 ccagcgctgc atttcacaag gtcaaggaag gcgaacttga tgctctggtc gcgacacagt
     2401 taaattcacg ttacatgatc gaccattact atcctaatga actttatcat tttcttattc
     2461 ccggcgttcc gaatgcatcg ctttcgttcg cttttcctcg cggagaaccg gaacttaagg
     2521 atattattaa taaagcactg aatgcaatcc ccccaagcga agttctgcgc ctgaccgaaa
     2581 aatggattaa aatgcccaat gtgaccattg acacatggga cctctatagc gagcaatttt
     2641 atattgttac gacattatcc gttttattag ttggcagtag ccttttatgg ggattttacc
     2701 tgttacgctc agttcgtcgt cgtaaagtca ttcagggtga tttagaaaac caaatatcat
     2761 tccgaaaagc actctcggat tccttaccga atccaactta tgttgtaaac tggcaaggta
     2821 atgtcattag tcataatagt gcttttgaac attatttcac tgcggattac tacaaaaatg
     2881 caatgttacc attagaaaac agtgactccc cctttaaaga tgttttttct aatgcgcatg
     2941 aagtcacagc agaaacgaaa gaaaatcgaa caatatacac acaggttttt gaaattgata
     3001 atggcatcga gaaaagatgc attaatcact ggcatacatt atgcaatctg ccagccagcg
     3061 atcatgctgt ttatatttgt ggttggcaag atattacaga gacgcgtgat ttaattcatg
     3121 cactcgaagt agagagaaat aaagcgatca atgcaactgt cgcaaaaagt cagtttctgg
     3181 caacaatgag tcacgaaata agaacaccaa taagctccat tatgggcttc ctggaacttc
     3241 tgtcgggttc tggtcttagc aaggagcaac gggtggaggc gatttcactt gcctactcca
     3301 ccggacaatc actcctcggc ttaattggtg aaatccttga tgtcgacaaa attgaatcgg
     3361 gtaactatca acttcaacca caatgggtcg atatccctac tttagtccag aacacttgtc
     3421 actctttcgg tgcgattgct gcaagcaaat cgatcgcatt aatttgcagc agtacatttc
     3481 ctgatcatta cctggttaag atcgaccctc aggcgtttaa gcaggtctta tcaaatttac
     3541 tgagcaatgc tctcaaattt accaccgagg gggcagtaaa aattacgacc tccctggttc
     3601 acattgatga caaccacgct gttatcaaaa tgacgattat ggattctgga agtggattat
     3661 cgcaggaaga acaacaacaa ctctttaaac gctacagcca aacaagtgca ggtcgccagc
     3721 aaacaggatc tggcttaggc ttaatgatct gcaaagaatt aattaaaaat atgcagggcg
     3781 atttgtcatt agaaagtcat ccaggcatag gaacaacatt tacgatcaca atcccggtag
     3841 aaattagcca gcaagtggcg actgtcgagg caaaagcaga acaccccatc acactacctg
     3901 aaaagttgag catattaatc gcggatgatc atccgaccaa caggctatta ctcaaacgcc
     3961 agctaaatct attaggatat gatgttgatg aagccactga tggtgtgcaa gcgctacaca
     4021 aagtcagtat gcaacattat gatctgctta ttactgacgt taatatgccg aatatggatg
     4081 gttttgagtt gactcgcaaa ctccgtgagc aaaattcttc cttacccatc tgggggctta
     4141 cagccaacgc acaggctaac gaacgtgaaa aagggttaag ttgcggcatg aacttatgtt
     4201 tgttcaaacc gttgaccctg gatgtactga aaacacattt aagtcagtta caccaggttg
     4261 cgcatattgc acctcagtat cgccaccttg atatcgaagc cctgaaaaat aatacggcga
     4321 acgatctaca actgatgcag gagattctca tgactttcca gcatgaaacg cataaagatc
     4381 tacccgctgc gtttcaagca ctagaagctg gcgataacag aactttccat cagtgtattc
     4441 atcgcatcca cggtgcggct aacatcctga atttgcaaaa gttgattaat attagccatc
     4501 agttagaaat aacacctgtt tcagatgaca gtaagcctga aattcttcag ttgctgaact
     4561 ctgtaaaaga acacattgca gagctggacc aggagattgc tgttttctgt cagaaaaatg
     4621 actaaatagc ggctcccaca atgttctaat gtgggagcta tttaccagca catcttacat
     4681 ttatgatgag aactcctggc gaatttgttc cccatgctgg tcgagcgttg ccgctcccgg
     4741 cataacatgc gggtccgcgc agccgctgat ttttatcgga ttacccggca tcattattcc
     4801 tccggcttca atcaacatat ttctcgcctg agtttgtggc aaattaatgg cctcagccac
     4861 acttaataac ggcgcgacgg gcacaccaac ttcatgtatt ttggctaacc aaacttcagc
     4921 tgcctgcgtt tttaacgttc gctcaatata ttgtttaaga atagcctggt tttgtacgcg
     4981 taaaatattg ctgctaaatc ggggatcatt aaccagttcc gtaagctcca gtgcctggca
     5041 taacgcagaa aaaagcttgt cattaccaca acaaatcgta atcggcttat cctgagtatc
     5101 gaaaacatca aaaggtgcca tgtagggatg gcgatttccc aggcgttgtg gtgacttccc
     5161 tgtcgcaata tatgccatca gaccatgctc cagaaaactc agcgtggcat caaacatcgc
     5221 tatatcgaca tgcgcccctc tctggctctt ttcgcggcca taaagtgcac tcactattcc
     5281 gctgaataaa tagacaccgc cgcatagatc cgcaagagag gtaccaacac gcactggcgg
     5341 agcatcaggg taccctgttt ccatcattat cccactcatt gcctgaatga tggtatcgta
     5401 ggcaggagca tcttttagcg gaccggtatg tccgaaacct gacgatgaag catatatgag
     5461 gcgcgggttg atttcttgta acctttccca tgaaaacccc agtttttcca ttgtacctgg
     5521 gcgaaaattc tcagctaata catcagcttg tttgagcata tttataaata tacttttatc
     5581 gtgatcattc tttaaatcaa gaaccacact ctctttgcca tgattaataa aactgtaata
     5641 gagtgactgt ccatccacat agggaccaaa tgtgcgggta tcatcaccat gacctggtgg
     5701 ctcaacttta attacccttg cgcccatatt acaaagaagt tgagttccga aaggtccatt
     5761 aaggacatgt gtcatatcga taactaataa gccttcaaac ggccctttgc tttcattatt
     5821 tgtcatttgc ttatgccttg aaaaaagccg aaggtgaaaa gacacttcgg catttatgta
     5881 aaatcaataa catgattaat tatgaaacca gtcgactgac ataaatccac aagggagccg
     5941 tgacgacaaa gcccagtacg ctcacagcca atgacgctgt accagtacgg gtgtagacat
     6001 taaaccggct ggcaataatg attccggaga atgccggcgg taatgcgcct gccagtacca
     6061 tcatctgcaa atgttcgctg ttcaaatgac atgccatacc aacgagaagc agcgccagtg
     6121 gcatcagaat caacttcagg aaggtgttat aagcaatttc ggcactgaac tcgaatttat
     6181 gtgctgccag agtcaacccc gcagcgaata ccgctacccc tgagttagcc ttcgcaatca
     6241 gattaaaggt cgggtcccat gctgccggaa tttttacccc aaccagcacc aggatcgttg
     6301 ccagaacagg tgcccatact actggctcct ttgccgcaga aattaatgcg ctcagattac
     6361 tattcttctt accatccgct cctgaggaag gattcagcaa atacagacca ataggaatag
     6421 taattgcgtt aacaataata gaaataattg ctaccactaa acctgttgat acggaatcac
     6481 cataaatagg atcgagaact gcaaacccca ggaatccaat ggtaggtgaa cccgcaatta
     6541 atgcacatac agcggcttct gcatgggtac gtttaaaaaa tttgtagcaa ccaaaccaag
     6601 agaaaaagaa acatccgaca ataaccacaa gtgatacaag ggtcagacga gtgtccgcaa
     6661 aaatcatttc cctgtttgcc cgggtaatag aaacaaatag agccgcagga agagcgtagt
     6721 ttaataccag tttattaaat gcgcgggctt gatcttctga aaaagtttct cgtctgccgc
     6781 taaaataacc caacagcatg attacgataa taggcaataa atcgccaata aaaaatgtta
     6841 gcataatagt atttccgata agggtcccct caccatcgca taatgagttg gcaagaggag
     6901 acgatgtcag tatttaatta ccagcgactt gttttgggtt aagtttggta atatggccac
     6961 tttcagttcc tgcagcaggg tcgatgacca cattaataat ggtcggtttg cgcgactgaa
     7021 taccggtggt taaagcatga cgaagttcat ctgtcgtggt gacgttatag ccaacgccac
     7081 gaaacgcatc cattaatttg tcataccttg catggtgcaa cagatccgtt ggtgatggtg
     7141 caccagcgcc actgagatca acaccgtctc ctctgtagat gccgccatta ttaaaaataa
     7201 cgatcgtcac cggcaggtta tatcgacaaa tcgtttcaat ttccatccca ctgaaaccaa
     7261 aagcactatc accttcaatg gcgacaaccg gagaaccaga agtcacgcta gcaccgatgg
     7321 catagcccat accgatgccc atgacacccc aggtaccaca atccagacga cgacgtggtt
     7381 tatacatatc aataatattt cgtgcattat ccagggtatt tgcaccttca ttaactaaat
     7441 aaatatcctg gttctcgcgc aatacgtcgc gcacagcact taatgcatta aagtaattta
     7501 atggttgagt atctgtactt aatttttcat gcattttttg tgcattttgc tgcttgtgga
     7561 tatttaaaat atcgcgccat accagtggag tcgtaaatgt gttttgtttc agttctgcca
     7621 gcataccttg catactggat gcaatatcac caacgactgg cacagcaatg gggcggttgc
     7681 tgtcaatttc ctgcggttca atatccagtt gaataaactg tgtatctgcc gcccatcctt
     7741 ttttaccgtg tgccaataac caattcagtc gtgcaccaac aagcatgaca acgtcagcat
     7801 ttgccagggc aaacgaacgc gcagctgccg cagaaagtgg atgcgtatct tcaaggatcc
     7861 ctttcgccat agacattggc aggaatggaa tctgagcact ttcaataaat tcacgaagct
     7921 gttcatcagc ttgtgaatac gccgcgcctt tgccaaggat aattaatggc cgttcagctt
     7981 ttgctaaaag cgaaattgcg ctagtgactg acttcgggca tggcaataat gctggcgacg
     8041 gattttcaac tttaacaatc gtggttaacg cttcgtcttt ttccatcgtc gcggccagga
     8101 catttgctgg caaatcaaga taaactccgc cagggcgacc cgatacagag acccggatag
     8161 cgcgtgccaa tgcaatgcca agatcctgcg gctgattaac gcgaaatgct gctttggcat
     8221 acggttttgc cgcattcatt tggtccagct cttcataatc accttgctgt aggtcgacga
     8281 tcgcgcggtc gctggagccg ctaatcatga tcatcggaaa accatttacc gttgcgttgg
     8341 ccaatgcggt caaaccattg aggaatcctg gcgcagaaac tgtcaggcag atccccggtt
     8401 tttgggtaag aaaaccgctt gccgcagcgg catagcctgc cgactgctca tgacgaaaac
     8461 caatataacg aatgccttcc gcctgggcat ggcgtgccat atccgtcaca ggaataccta
     8521 caacaccata aatagtgtca atattattct gttttaatgc ttcaacgatg atatgcatac
     8581 catctgtcat ttgaagttga tctgacatga ttaataacct cgaaattttt ataaattggg
     8641 agccagaagc ccccgttaat atcagatggc gtggttttgc ttcattgcag caatttcatc
     8701 gtcgctataa cccagctcct gcaatacagc agcggtatgt tcacctaata gcggcgcagc
     8761 tttaatatcc ggcgtaaagg cagagaattt cattggacaa ccaacggtca gatattttcc
     8821 acgcaacggt tgttccactt caacaacact gccactttgg cgcaaagagg gatcaagtga
     8881 aatttctttc atacttaaaa ccggtgcaca aggaatatca aactgagtca aataggccac
     8941 cgcttcatgt ttatcaatag tgacagtgta tttttcgatt tcagcaaaaa tatcgaaaat
     9001 atgtggctgt cgggcatggg ctgtactgta tgccgaatcg gtaatccatt ctggtttgcc
     9061 gatggcttta caggtgtttt cccagttttg ctcctgaata gtgaaataaa tataggcgtt
     9121 aggatcggtt tcccagcctt tacatttcag gatccagcca ggctgaccgc caccacctgc
     9181 attaccaccg cggggaactg catcaccaaa tgtaccattc ggatactgcg ggtattcttc
     9241 cagataaccc aatttatcga gacgctgctg gtcacgtaat ttcacgcggc aaaggttcaa
     9301 tacggcatcc tgcattgaca tggtgactcg ttgcccacgc cccgtttttt cgcgatgcag
     9361 caaagcagca agtaaaccga tcagcaaatg cattccggtg ttgctgtcac ccaacgctgc
     9421 agcgcttacc agcggcggac catcccaaaa acccgtagtg gatgccgcgc cacccgctgc
     9481 ctgagcaacg ttttcatagg cttttacatt cacataaggc gaacactcat caaacccttt
     9541 gatcgaacca aaaatcagac gtggattgat ttcttgaata tgctcccagg tgaagcccat
     9601 gtgatcaatg gcccctggat gaaagttctc gactaagata tcagcttcgc ggatcagctt
     9661 ttccattacc tctttgcctt ccgctgtttt ggtatttaac tcaatagaac gtttgttact
     9721 gttaagcatg gtgaagtaaa gcgcatcgat atcaggaata tctcgcagtt ggtgacgcgt
     9781 tacgtcacca acgccgggac gttcaatttt aataacgtca gcgccaaacc aggccagcat
     9841 ttgagtacaa gatgggccag attgcacacc ggtgaaatcg agaactttaa ttccttgaag
     9901 tggagttgac atagcaatac ctattagtta attaaatatg tgttaattta gcgtttattt
     9961 aaggcataac gccctctata aataatctgc aaaatatctt cgtagcggca ctcgatattt
    10021 ttaaattcct gtatcaagta ctgaaaatta tttcatcgcc acttccaatt cctttctgca
    10081 tggtaagtag tgaggacgaa aagaagaata tgataaatat ttattcgtgg taatcagaga
    10141 gagtcgcgac atttgtgatg ttattcttac aactgtaaga agcgccggtg atttacaagt
    10201 aagagtatga ttacgctttt taaagcgtac cataaagcat aacataataa atgatttgtg
    10261 atgacactgg attattgcgg tttactttga ggaaaataaa atgcgtgttt atagaaccgc
    10321 gagcgccaaa taattttatg accacaacct gtcgtattgt ggtcataaaa aattgtcggc
    10381 tggagggagt taaatccttt acctcaatat actgcctgtc agcattagtt cacaaacagt
    10441 gcttcgctat cgacgatgat tccatcttcc gcacctttaa gcgccaggtt tgcctcgtag
    10501 tattgctttt tatcaagcag tttctgcgca tcagcaagtg cattacgagt ctgtttaagc
    10561 ggcatcagat attggttttc cattacgcca acaccggcca ggcgaagctc ttccattgcg
    10621 ccctttttat cccctttcgc cattttttca ttggcaattt ttatcgcggc ttctttttcg
    10681 ggtgttgcta cataactctc agaaattccg acagatgcat taatgacaat atattggtca
    10741 tcattgagat tggtcttttt acctggctta gcgaactttg cccattcggt actgtcatct
    10801 gacagcaaag cggaagcttc attggtcagt tcttttgcct tttccggatc gccatgaaac
    10861 agagccaggc gcgctacctg cacgtcacgc atcgcatata aaccttgttc agagatacgc
    10921 tctgctgctg ccgttttctg ggtttgctga gtttgctgtt gtgccgctac cggtgcatta
    10981 tcagcagccc atacagttga agatgccaga attgctgtga ccatcgtagc cataattaaa
    11041 cgtttcatac ttacctcatt ctttaacagt tttagaaaac gccgccatca tattgggtga
    11101 gcatgaaatg agaaatcaga gagcaacggt cattttgaaa gggttatctt acaattgtag
    11161 ttattatcgc aaagcggcat ttagtcttac agttgtagga atatgcttcc tgtcgcagag
    11221 aactattctt atataccaca aagagatgtc atttattata ttgatgtaaa tattgattaa
    11281 ttatatgtaa atacagccat aacatcgtat ggttctcagt gaaccatttt caggataact
    11341 ttctatgaaa gtaaacttaa tacttttcag cttattttta ttggtctcta ttatggcatg
    11401 caatgttttt gcattttcca tttctggtgg tgtaagtgag aggagctata aagagactga
    11461 aaaaacatca gcgatgacaa ccacacactc tacaaaactt cagccatcac aggcgatttt
    11521 gtttaagatg agagaagatg cgccaccatt aaacctcaca gaagaaatca cgcctactta
    11581 tccgacaaag gcgaattatc ttattcatcc tgtgcgatag cgtctgtcac atggcaggtg
    11641 aacaatcatg tctgatgctg atgtgaacac ctgcccaaat gagccatcct gacccactat
    11701 cgcctgccat accccaccat aaagcgaatc atcaagccac aagccggggc aagaaacata
    11761 aatatccccg tcaccagttc attaccgcca gttaaccaca tggtgaagta ataaatgagt
    11821 gcagcgagga tcaccatgcc gaagaaagaa gagaccgccg ataccacgtt cagtacctga
    11881 ccgatgtagc cgcttacgca gacaatacac agggcgagaa acatccataa ataaatcata
    11941 gctcaacctc ctgccgcgct aaaaaacctg taacttcagt cactttaata gtaagacaat
    12001 ccttaacctc tggcaatttt tgctaagcat caatgaaaac agcacgttaa atgcaacgct
    12061 attgcgtgcg gaaaattttt tactttgcac gattaataat cagtagctga aagcagtcag
    12121 ccagtgataa agtagagcca ccgcattcat gctcctcgtg gttatgtcct gactagtctt
    12181 tacactcttt acaggaacca ttgtcgtaca tgatggccca accaattgag tgtaccgctg
    12241 tcgacatgga atcttctgcg atacaacaat tcgtatctac agaaggtaac tatgtttcca
    12301 caatgcaaat tttcccgcga gtttctacat cctcgctact ggctcacatg gtttgggctt
    12361 ggtgtactct ggctttgggt acagcttcct tatcctgttc tctgctttct cggcacgcgt
    12421 attggcgcaa tggcgcgacc attcctgaaa cgtcgtgaat ctatcgcccg taaaaacctg
    12481 gaactttgtt tcccgcagca ttctgcggaa gaacgcgaga agatgattgc cgaaaacttt
    12541 cgttcactcg gcatggcgct ggtagaaacc ggcatggcat ggttctggcc cgacagtcgc
    12601 gtacgtaaat ggtttgatgt tgaagggttg gataacccta aacgcgcaca aatgcaaaat
    12661 cgcggcgtaa tggttgtcgg cgtccatttt atgtcgctgg aactgggcgg ccgcgtgatg
    12721 ggactgtgcc aaccaatgat ggctacctat cgtccacata ataatcagct gatggaatgg
    12781 gtgcagaccc gtgggcgcat gcgctctaac aaagcgatga tcggcagaaa taatctgcgc
    12841 ggcattgtcg gtgcactgaa gaaaggtgaa gcggtatggt ttgctcccga tcaggattat
    12901 ggtcgtaaag gcagctcctt cgcgccgttc tttgcggtgg aaaatgtcgc cacaaccaat
    12961 ggcacctatg ttctctcccg tctctctggc gcagccatgt tgaccgtaac gatggtaaga
    13021 aaagcggatt acagcggata tcgtttgttt atcaccccag agatggaagg ctacccgaca
    13081 gatgaaaatc aagccgctgc ctatatgaac aagattatcg agaaagagat catgcgcgca
    13141 ccggagcagt acctctggat ccaccgtcgc tttaaaacgc gcccggtggg agaatcgtcg
    13201 ttgtacattt aacaaactgc tcttactctt tccctcctca aatcggccat aataacctca
    13261 tggtttttat ggcttttttc ttaaggtttt ttccttcgct gaatatctaa ttattaccgt
    13321 cagaaattaa actgtcgccg atatgagaca cttgtaagtt actaaaagta aataagaaaa
    13381 tgcctcttgt ctcgtttcag ttttaggcgt acattaacgc cgtcttgagc agtttagctc
    13441 agaattctgg ggtgatgccc acggtaaaat acagggcttt taaggtcgct ttgcccggtg
    13501 tcaactcact gtatcaggtg taatgaagtc attcaggcgt aacagtaatt acgcggagag
    13561 atgtaaagtg aaatatttct ttatgggcat ttcttttatg gtcatcgttt gggccggtac
    13621 ttttgccctg atgatctaaa agcaaactgt gcaatgaaaa acaggagcca tcaggctcct
    13681 gtttgcattt ctgacgacgg tgtttattcc gcgttttcgt gaatatgttt gctgctggcg
    13741 ggtagtaaac cgtcggcacg gaacatcgct ttaatccctc tgatggcctg acgaatacgg
    13801 tcgcggtttt caatcagtgc aaagcgaaca tgcgtatcac catagtcgcc aaagccaatc
    13861 cccggcgaga cacaaacctt cgcttcgtta aggagcttct tggcaaattc cagcgatccc
    13921 atggccgcat atggttccgg gattttcgcc cagacataca tcgaagcctt cggcatttcg
    13981 accatccagc ccgcttcatg cagcccttta accaatacat cacggcggcg tttgtactgt
    14041 tcggtaatgt cgcgcacgca ctgttgatcg ccctcgagtg ccgcaatcgc cgccacctgc
    14101 aacggcgtaa aagtgccgta atcgtgatag cttttgattc gcgccagggc actgaccagc
    14161 gttttgttgc cgaccataaa gccgatacgc cagcccgcca tgttgtagct tttcgacagc
    14221 gtaaagaact cgaccgccac atcgcgtgca cccggcacct gcataattga cggcgctttc
    14281 cagccatcgt agacgatatc ggcataggcc aggtcatgga ccaccagcac atcgtagcgt
    14341 ttcgccagcg ccacaacctt ttcgaagaac tccagttcca cgcattgcgc ggttgggttc
    14401 gacgggaagc cgaggatcat catcttcggt ttcggataac tttcacgaat ggcgcgctcc
    14461 agttcgttga agaaatcgac accttccacc agcggcactg agcgtacctg cgccccggca
    14521 atcaccgcgc cataaatatg gatcgggtaa cttggattcg gcaccagcac cgtgtcacca
    14581 tgatccagcg tcgccagcat cagatgcgcc aggccctctt tcgaaccaat agtgacgatg
    14641 gcttctgatt ccgggtcgat ttcaacgtcg tagcgatcct gataccagcg ggaaatggcg
    14701 cgacgtaacc gcggaatgcc gcgtgaagtg gagtaaccat gcgtgtccgg gcgctgggcc
    14761 acagtacata atttttcgac gatatgcggc ggagtcgcac cgtccgggtt acccatgctg
    14821 aaatcgataa tatcttcgcc gcgccgacgc gcagccattt tcagttcagc ggtgatgtta
    14881 aaaacgtagg gcgggagacg atcaatgcgc gtaaagcgac gttcagggcg agtgtcagcc
    14941 ataatttcct cagattaacg ttagcgcccg gaccgtccga gcgacgctgc cacaatggtg
    15001 gctgttttga aaatagcctg attaatttct gtctgtccag cgttttagaa catttttatc
    15061 catggcttta aatgaggaac tccacttcaa aaacaggaaa tttttctacg ctaacaagaa
    15121 aattaccacc aattttatta acattataat ttcaataaat ttaccagcca gaagtgcatt
    15181 atcaagagat acattgctaa tacaccattt atctttcaat ccccttttaa atttatggtt
    15241 tttcctcaaa aacggcctgc tggtcatcag ccttcaggtt acctatcata gaggtttaat
    15301 ccttattcag agtcacccgt gcacgaaata ttcaacatgc tgctggcggt cttcgatcgg
    15361 gcagcgttaa tgcttatctg cctgttcttt ctcatccgta tccgcctgtt tcgcgaactg
    15421 ttgcacaagt cggcgcactc cccaaaagaa ttgctcgccg ttaccgccat tttctcgctg
    15481 ttcgccctgt tcagcacctg gtccggcgtt cccgtagaag gctcgctggt gaacgtacgt
    15541 attatcgcgg tgatgtccga cgggattctg tttggcccgt gggtaggcat cattaccggc
    15601 gtgattgcgg gtattcaccg gtatttaatt gatatcggcg gcgtgacggc gatcccctgc
    15661 tttatcacca gcattctggc gggttgtata tcaggctgga taaacctgaa aatccccaaa
    15721 gcacagcgct ggcgcgtcag tattctcggc ggcatgttgt gtgagacgtt gaccatgatt
    15781 ctggtaattg tctgggcacc aactaccgcg ttggggatcg atatcgtctc taaaatcggc
    15841 attccaatga tcctcggtag cgtctgtatc ggctttattg tgc
//
LOCUS       DAEEKQ010000087        15584 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.087, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000087 DAEEKQ010000000
VERSION     DAEEKQ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15584
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.087"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..2067
                     /locus_tag="KLK57_002612"
     CDS             <1..2067
                     /locus_tag="KLK57_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBE0892944.1"
                     /translation="SGYVPPENWERGINAFYTSYYVSQYYSDYKASGNSKSTYVRFNS
                     GLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGFAQLLGTLRVGDMYTSSDIFDSV
                     RFRGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVTIEQNGFVVYQKEVPPGPFAITD
                     LQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQPGVSKYDFAAGRSHIEGASKHS
                     DFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGWNTRIGAISVDATKSHSKQDNGD
                     VFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVWANNKDNYRRDENDVYD
                     IADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGRSGSSKDYQLSYSNNLR
                     RISYTLAASQAYDENHHEEKRFNIFISIPFDWGDDVSTPRRQIYMSNSTTFDDQGFAS
                     NNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPVATVNGSYSQSSTYRQA
                     GASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQKYRTTNRNGVVVYDGMT
                     PYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTDQRKPWFIKALRADGQP
                     LTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQQGLSCTITFGKEIDES
                     RNYICQ"
     gene            2083..3117
                     /locus_tag="KLK57_002613"
     CDS             2083..3117
                     /locus_tag="KLK57_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HBE0892945.1"
                     /translation="MEIRIMLFILMMMVMPVSYAACYSELSVQHNLVVQGDFALTQTQ
                     IATYEHNFNDSSCVSTNTITPMSSSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTS
                     FTSGYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGG
                     KSWDACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGN
                     KATINSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLAGSNTILKGAENNGVGFML
                     ESNGSPVTFLNITDNSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALE
                     ATALINVKYD"
     gene            complement(3199..3537)
                     /gene="rcnB"
                     /locus_tag="KLK57_002614"
     CDS             complement(3199..3537)
                     /gene="rcnB"
                     /locus_tag="KLK57_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HBE0892946.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(3756..4580)
                     /gene="rcnA"
                     /locus_tag="KLK57_002615"
     CDS             complement(3756..4580)
                     /gene="rcnA"
                     /locus_tag="KLK57_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HBE0892947.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDYEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            4701..4973
                     /gene="rcnR"
                     /locus_tag="KLK57_002616"
                     /pseudo
     CDS             4701..4973
                     /gene="rcnR"
                     /locus_tag="KLK57_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
     regulatory      5065..5161
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5196..5984
                     /gene="thiM"
                     /locus_tag="KLK57_002617"
     CDS             5196..5984
                     /gene="thiM"
                     /locus_tag="KLK57_002617"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HBE0892948.1"
                     /translation="MQVDLLGSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGLGSFVPHFLDALWQLTQEVQA"
     gene            5981..6781
                     /gene="thiD"
                     /locus_tag="KLK57_002618"
     CDS             5981..6781
                     /gene="thiD"
                     /locus_tag="KLK57_002618"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HBE0892949.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            6846..7664
                     /locus_tag="KLK57_002619"
     CDS             6846..7664
                     /locus_tag="KLK57_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HBE0892950.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQVRLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKSTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            7716..8462
                     /locus_tag="KLK57_002620"
     CDS             7716..8462
                     /locus_tag="KLK57_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HBE0892951.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(8436..9401)
                     /locus_tag="KLK57_002621"
     CDS             complement(8436..9401)
                     /locus_tag="KLK57_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HBE0892952.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEELQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGEQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(9398..10402)
                     /locus_tag="KLK57_002622"
     CDS             complement(9398..10402)
                     /locus_tag="KLK57_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HBE0892953.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(10399..11676)
                     /locus_tag="KLK57_002623"
     CDS             complement(10399..11676)
                     /locus_tag="KLK57_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0892954.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQILGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSTDEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYQEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            11933..12985
                     /gene="fbaB"
                     /locus_tag="KLK57_002624"
     CDS             11933..12985
                     /gene="fbaB"
                     /locus_tag="KLK57_002624"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HBE0892955.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGTTIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13304..14158
                     /gene="gatY"
                     /locus_tag="KLK57_002625"
     CDS             13304..14158
                     /gene="gatY"
                     /locus_tag="KLK57_002625"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HBE0892956.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTYAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADVFYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            14187..15444
                     /gene="gatZ"
                     /locus_tag="KLK57_002626"
                     /pseudo
     CDS             14187..15444
                     /gene="gatZ"
                     /locus_tag="KLK57_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
     gene            15454..>15584
                     /locus_tag="KLK57_002627"
     CDS             15454..>15584
                     /locus_tag="KLK57_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="phosphoenolpyruvate-dependent sugar
                     phosphotransferase system; catalyzes the phosphorylation
                     of incoming sugar substrates concomitant with their
                     translocation across the cell membrane; IIB is
                     phosphorylated by IIA and then transfers the phosphoryl
                     group to the sugar; IIC forms the translocation channel;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBE0892957.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIAR"
BASE COUNT         4089 a         3728 c         3988 g         3779 t
ORIGIN      
        1 agtggctatg ttccaccgga aaactgggag cggggtatta atgcgtttta tacctcttat
       61 tatgtgagtc agtattacag tgactataaa gcgtcgggta atagtaagag tacatatgta
      121 cgttttaaca gcgggttaaa tttactgggg tggcaactgc attctgatgc cagtttcagt
      181 aaaacaaata acaatccagg ggtgtggaaa agcaataccc tgtatctgga acgtggattt
      241 gcccaacttc tcggcacgct tcgcgtgggt gatatgtaca catcaagcga tatttttgat
      301 tctgttcgct tcagaggtgt gcggttgttt cgtgatatgc agatgttgcc taactcgaaa
      361 caaaatttta cgccacgggt gcaggggatt gctcagagta acgcgctggt aactattgaa
      421 cagaatggtt ttgtggttta tcagaaagag gttcctcctg gcccgtttgc gattacagat
      481 ttgcagttgg ccggtggtgg agcagatctt gatgtcagcg tgaaagaggc ggacggttcg
      541 gtaaccacct atctggtgcc ttatgcagcg gtgccaaata tgctgcaacc cggcgtgtcg
      601 aaatatgatt ttgcggcggg tcgtagccat attgaagggg cgagcaaaca tagtgatttt
      661 gtccaggcgg gttatcagta tggttttaat aacttattga cgttgtatgg tggctcgatg
      721 gttgcgaata attattacgc gttcactttg gggactggct ggaatacacg cattggtgcc
      781 atttccgtcg atgccacgaa gtcgcatagt aaacaagaca acggcgatgt gtttgacggg
      841 caaagttatc aaattgccta caacaaattt gtgagccaaa cgtcgacgcg ttttggtctg
      901 gcggcctggc gttattcgtc gcgtgattac cggacattta acgatcacgt ttgggcaaac
      961 aataaagata attatcgccg tgatgaaaac gatgtctatg acattgccga ttattaccag
     1021 aatgattttg gtcgcaaaaa tagcttttcc gccaatatga gccagtcatt gccagaaggt
     1081 tgggggtctg tgtcattaag tacgttatgg cgagattact gggggcgtag cggcagtagt
     1141 aaggattatc agttgagtta ttccaacaac ctgcgacgga taagctatac cctcgcggca
     1201 agccaggctt atgacgagaa tcatcatgaa gagaaacgtt ttaatatttt tatatcgatt
     1261 ccctttgatt ggggtgatga cgtttcgacg cctcgtcggc aaatatatat gtctaactca
     1321 acgacgtttg atgatcaggg gtttgcctca aataatacgg gattatcagg aacagtaggg
     1381 agtcgggatc agttcaatta tggtgtcaac ctgagtcatc aacatcaggg aaatgaaacg
     1441 acagctggag cgaatttaac ctggaacgcg ccggtggcaa cagtgaatgg cagttatagt
     1501 cagtcgagta cttatcgaca ggctggagcc agtgtttcag ggggcattgt cgcctggtcg
     1561 ggtggcgtta atctggcgaa ccgtctttcc gaaacgtttg ctgtgatgaa tgcgccagga
     1621 attaaagatg cttatgtcaa tgggcaaaaa tatcgcacaa caaaccgtaa tggagtggtg
     1681 gtatacgacg gaatgacacc ttatcgggaa aatcacctga tgctggatgt gtcgcaaagc
     1741 gatagcgaag cagaattacg tggcaaccgg aaaattgccg caccttatcg cggcgcggtt
     1801 gtactggtta attttgatac cgatcagcgc aagccctggt ttataaaagc gttaagagcg
     1861 gatgggcaac cattaacgtt tggttatgaa gtcaatgata tccacggtca taatattggc
     1921 gttgtcggcc agggaagcca gttatttatt cgcaccaatg aaataccgcc atcggttaat
     1981 gtagcaattg ataagcaaca aggactttca tgcacaatca ccttcggtaa agagattgat
     2041 gaaagtagaa attatatttg ccagtaatag tcaggtggtt ctatggaaat tcgcataatg
     2101 ctatttatat taatgatgat ggttatgcct gtgagctatg cggcatgtta tagtgagtta
     2161 tctgttcagc acaatttggt tgttcagggg gattttgcac ttactcaaac acaaatagcg
     2221 acatatgagc ataattttaa tgattcgtca tgcgtgagta caaatactat cacccctatg
     2281 agttcgtcgg atattattgt tgggctttat aacgatacca taaaattaaa tttacatttt
     2341 gaatggacca ataaaaacaa catcacgttg tcaaataatc agaccagttt caccagtggt
     2401 tattcagtta cggtgacacc tgcggccagt aatgcaaaag tgaatgtttc tgcggggggc
     2461 ggcggttcag tgatgattaa tggtgttgcg acattatcca gtgcttcatc atcgacacgc
     2521 gggagtgccg cagtacaatt tctactgtgt ttattaggtg gcaagtcatg ggatgcatgt
     2581 gtaaatagct acagaaatgc attggcacaa aatgcaggtg tctattcctt taatctgaca
     2641 ttgtcataca acccgataac cacaacctgc aaaccggacg atttattaat tactttagac
     2701 agtattcccg tttcacaatt accagccaca ggtaacaaag caacaataaa tagtaaacaa
     2761 ggggatatta ttctgcgttg taaaaattta ttaggtcaac aaaatcaaac atcacggaaa
     2821 atgcaggtgt atttatcaag ttctgatttg ttagccggca gtaacacgat cctgaaaggt
     2881 gcagaaaata atggagtcgg atttatgctt gaaagcaatg gttctccagt cacattttta
     2941 aatatcactg ataacagcaa aggatacaca aatttaaaag aaattgcggc aaagtcaaaa
     3001 cttacagata caacggtttc aattccgata actgccagtt actacgtcta cgatacaaac
     3061 aaagttaaat ctggcgcact ggaggcaacc gcattaatca acgtgaaata cgactaataa
     3121 caagcaagac gagcaagtgg ctaataataa aaaataacaa ggtgtctgca tttccccctc
     3181 atgatgaggg ggcttttttt agcgatgata aaaaatctca ccgtcgtagg ctttaatgat
     3241 tttaccgtcg gtgtcgctga tcaacacgta cgcgccaccc atataggtcc agtgcgtccc
     3301 ggcatcaggc gcgggcaggt tacgtaactg ccactgctta atgttgtact catcggtgcg
     3361 gtacatctct gggacggtat cacctggttt gaaatgctta aagtcagcaa tgaaactact
     3421 taactcatac ttagaaattc ccgaggtatt ggtcgaaccc gcggtggctg gtgcggccca
     3481 ggcggcactg gtaaccagca aaagcacacc cagcaacatc ttatttttaa tggtcataaa
     3541 ctctccactt tggggatctg taatatcagt cgcatatctt tcctgccata caatataact
     3601 gacaagcgat attttgttag tgatcccatg atttcgtaaa aagtgtaaat gaaaggatgc
     3661 cctaaaagca tccttagcga gcagagagtc agcggaaata gctgatttaa gaatcatcct
     3721 gggctgaaga taacatgcaa gcctaaagga ttgagttatc gcattatgcc catgaagcca
     3781 tgtacgccca tatacacacc gactaagcca atcaacagac tggaaaaata gggggcgcgt
     3841 ttagcgagag tgttaaatcc gctccagcgt tttgcgacct gctgaacgct gattgctgcg
     3901 ccaacgccta cggtgacaag cgttaacgcc aggccaatgc tgaaactgac gaccagtgtt
     3961 gcgcccagtg tcagggcttt caactgaatg catatcaaca gcacggtaat tgctgccggg
     4021 caggggataa ggccaccggt taagccaaat agcaaaattt gccagttggt gacctctcta
     4081 ccatcaaagc gtcgtttaat gtcattggca tgggctcgtg catgggcatc ctgatactcg
     4141 ccatgttcgt gatggtgatg atgtccatgg tcgtggtgat cttcgtgatc gtgatgatga
     4201 tgctcataat catactcgtg catattctcc agccagttgc gttcgccgcg ccaggtacgc
     4261 cagaacatcc agaacgcggt gccaataatg atcactgcgg aaatcagctg gagccacggt
     4321 tctgctgatt gagcagtaaa gcgcttgctg atcaccatcc cgccaaaggc aattaaccag
     4381 accactgcgg tatgcgaaat agttgctgcc agtccgagca tcaccgcttg tttaatggtg
     4441 cctttgatgg cgatgataaa cgccgccatc atcgtttttg agtgccctgg ttccaggcca
     4501 tgaagcgcac caagtaagat ggcgctgggg atgaagaacc aggcgtttcc ttgctgaaga
     4561 agagttgtaa attcggtcat gataataatt cttagtatta attcggcaat ctgattctac
     4621 tccccccagt acctgatact accccccagt agattaatag tgctatgata ttttcatatt
     4681 cttgttaacc aggtgttgcc atgtctcata caatccgtga taaacagaaa ctgaaagcgc
     4741 gtgccagtaa gattcagggc taggtcgtgg cgctcaagaa aatgctcgac gagccgcatg
     4801 aatgcgctgc agttttacaa cagattactg ctatccgtgg cgcggtaaac ggtctgatgc
     4861 gggaagtgat taaaggtcat ctgacggaac acatcgttca ccagggggat gagctaaaac
     4921 gtgaagaaga tctggatgtc gttctgaagg tgctggattc atatatcaaa taatttatta
     4981 acgcgattgt aaaactgccg tttttcctcg tttacaacgc gtgcgctgga cattaccatc
     5041 ctcctctgcg atttatcatc gcaaccaaac gactcggggt gcccttctgc gtgaaggctg
     5101 agaaataccc gtatcacctg atctggataa tgccagcgta gggaagtcac ggaccaccag
     5161 gtcattgctt cttcacgtta tggcaggagc aaactatgca agtcgacctg ctgggttcag
     5221 cgcaatctgc gcacgcgtta cacctttttc accaacattc ccctcttgtg cactgcatga
     5281 ccaatgatgt ggtgcaaacc tttaccgcta ataccttgct ggcgctcggt gcatcgccag
     5341 cgatggttat cgaaaccgaa gaggccagtc agtttgcggc tatcgccagt gccttgttga
     5401 ttaacgttgg cacactgacg cagccacgcg ctcaggcgat gcgtgctgcc gttgagcaag
     5461 caaaaagctc tcaaacaccc tggacgcttg atccagtagc ggttggtgcg ctcgattatc
     5521 gccgccattt ttgtcatgaa cttttatctt ttaaaccggc agcgatacgt ggtaatgctt
     5581 cggaaatcat ggcattagct ggcattgcta atggtggacg gggagtggat accactgacg
     5641 ccgcagctaa cgcgataccc gctgcacaaa cactggcacg ggaaactggc gcaatcgtcg
     5701 tggtcactgg cgaggtggat tatgttaccg atggccatcg cgccgttggc attcacggtg
     5761 gcgatccgtt aatgaccaaa gtggtaggaa ctggctgtgc attatcggcg gttgtcgctg
     5821 cctgctgtgc gttaccaggc gatacgctgg aaaatgtcgc atctgcctgt cactggatga
     5881 aacaagccgg agagcgcgca gtcgccagaa gcgaggggct aggcagtttt gttccacatt
     5941 tccttgatgc gctctggcaa ttgacgcagg aggtgcaggc atgaaacgaa ttaacgctct
     6001 gacgattgcc ggtactgatc cgagtggtgg tgcggggatt caggccgatc ttaaaacctt
     6061 ctcggcactt ggcgcttatg gttgctcagt tattactgca ctggtggcgc aaaatacccg
     6121 tggcgtacag tcggtgtatc gcattgagcc tgattttgtc gccgcccagc tcgattcggt
     6181 gttcagcgat gtgcgaatcg ataccactaa aatcggtatg ttggcggaaa ccgatattgt
     6241 tgaagcggtg gcagaacggt tgcaacgtta tcagatccaa aacgtggtac tcgacaccgt
     6301 tatgctggca aaaagcggcg acccactgct ttcaccttcg gcagttgcca cgctacgcag
     6361 tcgattattg ccacaggttt cattaataac gccaaacttg cccgaagcag ccgccttgct
     6421 cgacgcgcca cacgcgcgca ccgaacagga aatgctggaa caagggcgat cgctgttggc
     6481 gatgggctgt ggcgcagtgc taatgaaagg tggtcatctg gatgatgagc aaagcccgga
     6541 ctggctgttt acccgcgagg gtgaacaacg gtttaccgca ccgcgcatta tgaccaaaaa
     6601 cacccacggt actggttgta cactctctgc ggcgttggct gcactacgcc cgcgccatac
     6661 aaactgggct gacaccgtac aggaggcaaa aagctggctt tcatcggcgt tagcccaggc
     6721 cgacacgctg gaagttggtc acggtattgg tccggttcac cacttccacg cctggtggtg
     6781 agcaccgtac tatagctgct ttggttattg tcagcctttg gcactgacat tcagggatga
     6841 aaccaatgca gctaagaatt accagtcgaa aaaaactcac cgctctatta tgcgctcttg
     6901 ggctaatttc catcgttgca atttatccgc gtcagacggt gaattttttc tattcgacag
     6961 cagttcagat tacagactac atccacttct acggttatcg cccggttaaa tctttcgcta
     7021 ttcgtattcc tgccagttac accattcacg gaatagatgt ttcgcgctgg caggagcgga
     7081 tcgactggca gcgtgtggca aaaatgcgcg ataacggtat tcgcttacaa tttgccttta
     7141 ttaaggcgac ggaaggcgaa aagctggtgg acccctattt ttcgcgtaac tggcaactaa
     7201 gccgcgaaaa tggcttgctg cgcggggcat atcattattt ctccccgtcg gtagcagcac
     7261 cggttcaggt gcgattattt ctgcaaacgg tagatttctc acaaggcgat ttccctgcgg
     7321 tactggacgt agaagagcga ggaaaattat cggcaaaaga attacgcaag agggtaagtc
     7381 agtggctaaa aatggtcgaa aaaagtacgg ggaaaaagcc gattatttac tcaggagccg
     7441 ttttttatca cacgaatctg gcgggctatt tcaatgaata tccgtggtgg gtggctcact
     7501 actatcaacg tcgtccggac aatgacggca tggcctggcg cttctggcag cattccgacc
     7561 gtggacaggt agatggcatc aatggtccgg tggattttaa tgtgtttaat ggcacggtgg
     7621 aggagttgca gggattcgtt gatgggatta aagaaacgcc ttaaataaga agcagtgata
     7681 tactcccacc agactccctg gcaggaaaaa gacaaatgga acaagcgcat acccagctga
     7741 ttgcccaact gaacgaacgt attttagcgg cggataacac gccgctttat attaagtttg
     7801 ccgaaacggt aaaaaatgcc gtgcgcagcg gggtgctgga gcatggcaat attttgcccg
     7861 gtgagcgtga tttaagtcag ttaaccggcg tttcgcgcat tacggtgcgc aaggcgatgc
     7921 aggcgctgga agaagagggc gtggtgacgc gttcgcgcgg ttacggcacg cagatcaaca
     7981 acatcttcga atactcgttg aaagaagcgc gaggtttttc tcagcaggtg gtgttgcgcg
     8041 ggaaaaagcc cgatacgcta tgggttaaca agcgtgtcgt gaaatgtccc gaagaagtcg
     8101 cgcagcagct ggcggtcgaa gcaggaagtg atgtcttttt gcttaagcgt attcgctatg
     8161 tcgatgaaga agcggtatcg attgaggaat cgtgggttcc ggcgcattta attcatgatg
     8221 ttgatgccat cgggatttca ctttatgact atttccgcag ccagcacatt tacccacaac
     8281 gtacgcgttc ccgcgttagc gcccggatgc cggatgccga gtttcagtca catattcagt
     8341 tagatagcaa aataccggtg ctggtgatca agcaagtggc gcttgaccaa cagcaacggc
     8401 ctattgagta cagcattagc cactgtcgca gcgatctata cgtttttgtg tgcgaggagt
     8461 agttcctcgc gcgttggcgc acaatcaccg ccccgatgcc cgacaaccca cgacgccact
     8521 gcattgccca gtaatacggc atccgccagt ggcagaccag aggccagccc ggcaagtacg
     8581 ccacctgcat gactgtcgcc cgccccaatg gtgtctacaa cttgcgttgg aaatgccgga
     8641 atgcagccag aagcgtcgtt gctgaaatac catgcgcctt ctttatcgag acgaacgatc
     8701 aacggcgcgg caaatttctc ctgccattgc tcgccaagtg ttgttatctc tgcggataaa
     8761 gcaaaacgtt cggcggcaat ctcagcctct tgacgattga gcgacactaa aggtcgacag
     8821 gccatgatcc gcgccagtaa tgcatccggg atatcgccaa tacgtgggcc aaaatcgata
     8881 aacggcgtca cgtcttgcag ctcttccagc cattccacta acaattcgcc gcagggcgag
     8941 gccagttgat aaccggaaaa atagagcagg ctgccaggcg caacggttaa tcgcgccagc
     9001 cactggcgat tccactgatt ttccacaccg ctgaatgaca taaaagtgcg ttcgccatcc
     9061 ggctcaacca gcgccagaca ccagccgtta tcaccttcgg cgttatcgat caggctgatt
     9121 aagccctctt ttgccatccg gttgcgaata atctccgccc acacgccctg accgagcggc
     9181 aaggcattac ccgcttcgat gccgaggcgc tttaacgcca cggcaatatt cagtgcgcag
     9241 ccgccaacgt taacgctctg ctgtttcagt tcgatatcgc acccacgcca ggggagggca
     9301 taagcgtcgg cgatcacgtc aatgaccgcc gcgccgacga ccatcaccgg ctgacgcgtg
     9361 gttaattcag gcagcagcgt gtgtaatcga gcgccgctca taccgcctcc cgttgttgac
     9421 gatatttcgc cagcgctgtg gcatagcggt tgaaatcaag ctgatttacc gcatccagtt
     9481 ccgcctttaa cgcaggatcg atagcgttaa cgccatgcaa cgcgccgcaa attgccgtcg
     9541 ccatagcacc gatggtgtct gtgtcgccgc caaggttagc gcacaggacg gcgcagcgat
     9601 tcgggtcggt ttgtgctagt tcaaccagcg caatggcgca cggaacggac tcaatagtgc
     9661 tggtacctgc gccaacgacc tggtaaagct gttcgctggc ggattcggtg ccgtcggcat
     9721 tgcgcacaat tttcagcgca atctccagcc gcgccgccag tgaggcgctg aacgtagtga
     9781 tgcgtttttg ctgtgcatgt cgcgcaattg aaggcaggga atcaacaatc gctgaccagc
     9841 tttctccgtc aatggcacga gaaatcgccc atgcgatgac taccgcgcct gcaaccgcca
     9901 gatcggattt atgggtcggg ctggacgcca gcgctacatc atcaataaag gaatcaacat
     9961 cacgcgccgg aagcaaacaa cctaatggcg agacgcgcat cgctgcgccg ttggtcacgc
    10021 cgttgttttc cagttcagca acgggtttac cgtcgcgaat ggcgttaagc gcaatcttcg
    10081 aggtcggacc tagtacgttt ttgttaaagg cgtcgaaacg cagcgcccag tcgagaatat
    10141 tacgcccaat cagatccgga tcgatcttgc cttcacgttc cagtaacgca tccgccagac
    10201 acagcgccat cgaggtatcg tcggtgaatt cggcgcggtt aaaataacag gccgcgttat
    10261 tctcctttgg tccaggaaga aaacggtcaa tccagccaaa gtgtgcttta acgcggctgc
    10321 gtggccaaag ctcggagggc atccccatcg catcccctaa cgcctgccca taaagagcac
    10381 cgagaatacg ttctgttttc atttaacttc cccttgtgtc aacgcaatat cgcgatcatc
    10441 gaccttgata gccgtaattt cgttgtcgga ttcgcggaaa aaaatcatga acagcacggc
    10501 gataatggca atcatcaccg cgccgaaagt ccacatcccg gaccagttga aagtcagtcc
    10561 gtttaccggt tcctgataag cgaacatctt ttccatcatc acaccgccaa gacgatagcc
    10621 gagcaaactg ccgaagccct ggcagcagag cgtgatcagc ccctgcgcag cggtacgcat
    10681 atgcacgggg gcttttttat cgacatagat gtaagcggta acgtagtaaa aatcgtaact
    10741 tacgccgtga agcaaaatac cgaggaacag taacgcgtag gtgaaatatt catccgtact
    10801 accgtaaata aagaagccat agcggatcgc agcggtgacc agaccaagca ataatacctt
    10861 tttgatacca aagcgtttag tgaaaaacgg caatgccagc ataaagaaga tttcagagaa
    10921 ctggccgagc gtcatccagc cggtggcgtt tttcatgcca acttcggtca gataaccgtt
    10981 ggcaaagatg taatagaacg ccagtggcat cgcaaacagg aatgaacaga agaaaaagac
    11041 gaggaagttt ttatcgcgca gcaggatcag cgcatccagg ccgagcatga ctttaatatc
    11101 cattttgccg gtgctttttg gtggcgtgtc gggcaggaaa aacgcaaaca caccgagcag
    11161 agcagaactt ccggcggtaa tcagcagcgg gatgttagtc ggtgagatat cggcataccc
    11221 cagtatttgc ggcaagaaac cacatgccag accggaggca atccagccga tagtgcccat
    11281 cacacgaatg cgcgggaaat cacgctccac atccggcacg ttggcaaaag cgatgctgtt
    11341 agtcagcgca atggtcggca tataggttag agagtaagcc agcagtaacg gaaagaaccc
    11401 ggcaaaagtg gtctgttgcg cagcgaaata catcagcagc gcgcctgcga acatcaatac
    11461 cgccagcact ttttgcgccg agaaaaagcg gtcagtgatg gagccaacca gaattggcga
    11521 caggatcgcc gcaatggcgg tacaggcata cgaccagcca atttctccgg cactaaaacc
    11581 gcttttactt aaccacaacc acaatggcac aaaccacgcg ccccagataa accattcaac
    11641 aaacatcatg aacgacagct ttgctgttgt tttcatcttt taattccttc atgacaggta
    11701 atgtaaggta cgcgatgaca ataccactta acaatacctt ttaaatacct ttgcggtgaa
    11761 aacttgaccg cgctaacatt ttttctgatg aatcgagcca acagaaaacg ctgaaaaaac
    11821 atccaaaaga tggaaaaact cgcccgtaac gaccaggatt atgattgccc gcaaaaggcg
    11881 ggccaggaca atccatagcc gatatccaat cggaatttac gggagcatag taatgacaga
    11941 tattgcgcag ttgcttggca aagacgccga caacctttta cagcaccgtt gtatgacaat
    12001 tccttctgac cagctttatc tccccggaca tgactacgta gaccgcgtaa tgattgacaa
    12061 taatcgcccg ccagcggtgt tacgtaatat gcagacgttg tacaacaccg ggcgtctggc
    12121 tggcacagga tatctttcta ttctgccggt tgaccagggc gttgagcact ctgccggagc
    12181 ttcatttgct gctaacccgc tctactttga cccgaaaaac attgttgaac tggcgatcga
    12241 agcgggctgt aactgtgtgg cgtcaactta cggcgtgttg gcgtcggtat cgcggcgcta
    12301 tgcgcatcgc attccatttc tcgtcaaact taatcacaac gagacgctaa gttacccgaa
    12361 tacctacgat caaacgctgt atgccagcgt ggagcaggcc ttcaacatgg gcgcggtggc
    12421 ggttggtacg actatctatt ttggctcgga agagtcacgt cgccagattg aagaaatttc
    12481 tgcggccttt gaacgcgcgc acgagctggg catggtgacc gtgctgtggg cctatttgcg
    12541 aaactccgcc tttaagaaag atggcgttga ttaccatgtt tccgccgacc tgaccggtca
    12601 ggcaaaccat ctggcggcaa ccatcggtgc agatatcgtc aaacaaaaaa tggcggaaaa
    12661 taacggcggc tataaagcaa ttaattacgg ttacaccgac gatcgtgttt atagcaaatt
    12721 gaccagcgaa aacccgattg atctggtgcg ttatcagtta gctaactgct atatgggccg
    12781 ggctgggttg ataaactccg gcggtgctgc gggcggtgaa actgacctca gtgatgcagt
    12841 gcgtactgcg gttatcaaca aacgcgctgg cggaatgggg ctgattcttg gacgtaaagc
    12901 gttcaagaaa tcgatggctg acggcgtgaa actgattaac gccgtgcagg acgtttatct
    12961 cgatagcaaa attactattg cctgatacgc ctcatacgaa cagcaccacc ccgtaggacg
    13021 gataaggcgt ttacactgca tccggcaacc tatgcctgat gcgacgcgga agcgtcttat
    13081 catgcctaca tagcactgcc acgtatgtcg gaaaaggcgt ttacaccgca tccggcataa
    13141 aaacacgcgc actttgctac ggcttcccta tcgggaggcc gtttttttgc ctttcactcc
    13201 tcgaacagtt ttcatattgt cgttttgtga tcgttatctc gacatttaaa aacaaatcat
    13261 ttcattatat tttgaaatcg aaaacaagca acaggatatg aaaatgtacg tggtatcgac
    13321 aaagcagatg ctgaacaacg cacagcgcgg cggttatgcg gttccggcat tcaatattca
    13381 caatctcgaa acgatgcaag tggtggtgga aaccgctgcc aacctgcatg cgccggtcat
    13441 catcgccgga acgcctggca cattcactta tgctggtaca gaaaatctgt tggcgctggt
    13501 cagcgcgatg gcgaagcaat atcaccatcc actggcaatt catctcgacc atcacacgaa
    13561 atttgacgat atcgctcaga aggttcgttc tggcgtgcgc tcagtcatga ttgacgcctc
    13621 gcatttgcct tttgcgcaaa atatttcacg ggtcaaagag gtggtggatt tttgccatcg
    13681 ctttgatgtc agcgtcgaag cggagctggg gcaacttggc ggccaggaag atgatgtgca
    13741 agtcaatgaa gccgatgttt tttataccaa ccccgctcag gcgcgtgaat ttgccgaggc
    13801 aaccggaatt gattccctgg cggtcgccat cggcacggct catgggatgt atgccagcgc
    13861 accggcgctt gatttttctc gactggagaa cattcgccag tgggtgaact taccgctggt
    13921 gctgcatggc gcgtcagggt tatcgactaa ggatattcag caaaccatca aactggggat
    13981 atgcaaaatc aacgttgcaa cggagctgaa aaatgccttc tcgcaggcgt taaaaaatta
    14041 cctgaccgag caccctgaag cgaccgatcc ccgggattat ttgcagtcgg ctaaatccgc
    14101 aatgcgcgat gtggtgagca aagtgattgc cgattgtggc tgcgagggca gggcataacg
    14161 cacctgccat ttaacaagga aaaaacatga aaacgttaat tgcccggcat aaagctggtg
    14221 aacatatcgg catatgttca gtctgttctg cccatccgtt ggttatcgaa gcggcgctgg
    14281 catttgatcg caacagcacg cgcaaagtgc tgattgaagc aacgtcaaac caggtcaatc
    14341 aatttggcgg ttataccgga atgacaccgg cagactttcg cgaatttgtt tttacgattg
    14401 ccgataaagt cggatttgca cgcgaacgca ttattctcgg cggcgatcat ctggggccaa
    14461 actgctggca gcaagaaaat gcggatgcgg cgatggaaaa atccgtcgag ctggtaaaag
    14521 catatgttcg tgctggcttc agtaaaattc atcttgatgc gtcaatgtcc tgcgcggggg
    14581 atcccatacc gttagcacca gaaacggttg cggaacgagc tgctttgctt tgctgcggaa
    14641 agtgtggcga cagattgcca gcgtgagcaa ctgagctatg tcattggcac tgaagttccg
    14701 gttccgggcg gtgaggccag cgccattcag tcagtacaca tcacccatgt tgaagatgcc
    14761 gccaatactt tacgtacgca tcaaaaggcc tttattgccc gtgggctgac agaggcgtta
    14821 acacgcgtga ttgccatcgt ggtgcagccg ggcgtggaat ttgatcacag caatattatc
    14881 cattatcagc cgcaggaagc gcagccgctg gcgcaatgga tagaaaacac ccgaatggtt
    14941 tatgaagcac attctaccga ttaccagacc cggacggctt attgggaatt agtccgcgat
    15001 cactttgcaa tattgaaagt cggtcccgca ttaacctttg ctttacgcga ggcgatattt
    15061 gcactggcac aaattgagca ggaacttatc gcccctgaaa atcgcagcgg ttgcctggcg
    15121 gtaattgaag aagtgatgct cgacgaaccg caatactgga aaaaatatta tcgtacgggt
    15181 tttaacgatt cattactgga tattcgttac agcctgtcgg atcgtattcg ttattactgg
    15241 ccgcatagtc ggattaaaaa tagcgtcgaa acgatgatgg tgaatcttga aggcgtggac
    15301 atcccactgg gcatgattag tcagtatctt cccaaacaat ttgaacgcat tcagtccggg
    15361 gaattatcag caataccgca tcagctgatt atggataaaa tttatgatgt tttgcgcgcc
    15421 tatcgctacg gctgtgcgga ataaggacgg tatatgacta acctgtttgt tcgtagcgga
    15481 atttcttttg tcgatcgtag cgaagtttta acccatatcg gtaatgagat gctcgccaaa
    15541 ggtgtggttc atgatacctg gccacaggca ttaattgcca gaga
//
LOCUS       DAEEKQ010000088        15526 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.088, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000088 DAEEKQ010000000
VERSION     DAEEKQ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15526
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.088"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="KLK57_002628"
     CDS             complement(97..1209)
                     /gene="ydiK"
                     /locus_tag="KLK57_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HBE0892958.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGSIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            1598..4654
                     /locus_tag="KLK57_002629"
     CDS             1598..4654
                     /locus_tag="KLK57_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HBE0892959.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKTNTTIGRIYNTVY
                     QRCRLQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            4651..5061
                     /gene="menI"
                     /locus_tag="KLK57_002630"
     CDS             4651..5061
                     /gene="menI"
                     /locus_tag="KLK57_002630"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HBE0892960.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            5161..5349
                     /locus_tag="KLK57_002631"
     CDS             5161..5349
                     /locus_tag="KLK57_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HBE0892961.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSTELKEEIYFAWRLGVH"
     gene            5897..6265
                     /gene="sufA"
                     /locus_tag="KLK57_002632"
     CDS             5897..6265
                     /gene="sufA"
                     /locus_tag="KLK57_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HBE0892962.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            6274..7761
                     /gene="sufB"
                     /locus_tag="KLK57_002633"
     CDS             6274..7761
                     /gene="sufB"
                     /locus_tag="KLK57_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HBE0892963.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            7771..8517
                     /gene="sufC"
                     /locus_tag="KLK57_002634"
     CDS             7771..8517
                     /gene="sufC"
                     /locus_tag="KLK57_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HBE0892964.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            8492..9763
                     /gene="sufD"
                     /locus_tag="KLK57_002635"
     CDS             8492..9763
                     /gene="sufD"
                     /locus_tag="KLK57_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HBE0892965.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPLSHHFAHNDLLLAEDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKMAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            9760..10980
                     /gene="sufS"
                     /locus_tag="KLK57_002636"
     CDS             9760..10980
                     /gene="sufS"
                     /locus_tag="KLK57_002636"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HBE0892966.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEIITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            10993..11409
                     /gene="sufE"
                     /locus_tag="KLK57_002637"
     CDS             10993..11409
                     /gene="sufE"
                     /locus_tag="KLK57_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HBE0892967.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLTELRDEDRSP
                     QNSIQGCQSQVWIVMRQNAHGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVSFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            11558..12562
                     /gene="ldtE"
                     /locus_tag="KLK57_002638"
     CDS             11558..12562
                     /gene="ldtE"
                     /locus_tag="KLK57_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HBE0892968.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIVPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="KLK57_002639"
     CDS             complement(12625..12861)
                     /gene="lpp"
                     /locus_tag="KLK57_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HBE0892969.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="KLK57_002640"
     CDS             complement(13171..14583)
                     /gene="pykF"
                     /locus_tag="KLK57_002640"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HBE0892970.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
     gene            15140..15349
                     /gene="fumD"
                     /locus_tag="KLK57_002641"
     CDS             15140..15349
                     /gene="fumD"
                     /locus_tag="KLK57_002641"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HBE0892971.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         3927 a         3747 c         4100 g         3752 t
ORIGIN      
        1 agctgaatgg cggtaagcag gaaaatatgg ggaagagcaa gcaaatatca attagcggtg
       61 attaacaatc aggcggcaga gccgcctgat gagaaattac ttattcggtt tctcgatttc
      121 gccaagttct tcgagaattt cttccggttg gtccgtcggc ggcgggactt cttccaccca
      181 cgcggcaaac agacgccagg aaacggctaa cagaaccgga ccaataaaca gaccgatcat
      241 cccgaaagca atcaaaccac caataacgcc agagaggatc aggatcagcg gtaaatcggc
      301 acccatgcga attaacattg ggcggatgac gttatccagc gtgccaacca caccgctcca
      361 cactaacaat accgttcccc aggtggtatc gccggtccag tagagccaga taatcgccgg
      421 aatcagtacc ggcaacgggc caagctggac aaggcaggag aggatcatta acaccgttag
      481 caacgttgca taaggtacgc cggatacggc gaggccgata ctgccaagca ctgcctgtac
      541 taacgccgtc accaccacac ccagcgccac cgcgcggata gcctgtgccg ccagcagcac
      601 ggcggcatct ccgcgaacgc ctgccagacg ggttgcaaaa tggcgaatgc cttgtgccac
      661 ctgttcaccg cgccagtaca gcagggcact gaagagaagc atcagcgcac aatgcaccat
      721 aaagcgcccg atatgcgccg cctgcccaac gaaccaggtg gtggtggtgc caatataagg
      781 gcggactttc gccatgatcg ccgtaccccc catatccagc aagttgtgcc agcctgcata
      841 cagcttcgcg ccaatcaccg gaatggtatt aagccacgcc agatcgggta acgtcatgtc
      901 gccgctggaa atggctttaa ttagcgggcc gctgccgtcg acgatactgt taaccagcaa
      961 agcaataggg atgataaaca ccatcactaa taacagcgtc atcaccagaa cggcgagaga
     1021 gcggcggcca aacatgatct tttgcaaacg taacaatacc ggccaggtgg cgataaccac
     1081 cgtaccggcc catgcaaagc cgagaataaa gggttgaaca atccacagac atgccacaat
     1141 catgatggct aaaaacagca ccgaaagcag aatttgtgcg acatccctgg gctgacgaac
     1201 atttaccata actacttttc accttttttg tgcgtcaaat cgtcgacgcg acgtgaacac
     1261 gcgaaactca cctgcataat gatgagcaat ttcagcggtt tttaacaggc cgattctgcc
     1321 cttaattctg atgggcgtgt tagaaaaaaa tgtggtacaa caactcaggc aacacgcaaa
     1381 cgatttactc gtcgtatttc aagttgcatg cgctgcggct acgttcgttc accccagtca
     1441 cttactattg taagctcctg gagttcactc gcttgccgcc ttcctgcaac tcgaaatcca
     1501 tagagtaaat aaacatcacc tttcagtgcg ctattgcagc cgcctgaaag aatagtgtat
     1561 cgacctacat cacagaccgc aggaaagggt caatataatg attccacaga tttcccaggc
     1621 acccggcgtc gttcaactgg tgcttaattt tttgcaagag ctggagcaac aaggttttac
     1681 cggcgatacg gcgacaagtt atgccgatcg tctgacaatg tcgaccgaca acagtattta
     1741 ccaacttctc cccgatgcgg tggtatttcc gcgttcaacc gcagatgtgg cgctgatcgc
     1801 ccgtcttgcc gcgcaggaac gctattcatc gctgatcttt accccccgcg gcggcggcac
     1861 cggcactaac ggtcaggcgc tcaaccaggg gattattgtt gatatgtccc gccatatgaa
     1921 ccgcatcatc gaaattaacc ctgaagaggg ctgggtgcgc gttgaggccg gggtgataaa
     1981 agatcaactc aatcagtacc tgaaaccgtt cggctacttt tttgcaccag aactttcgac
     2041 cagcaaccgg gcaacgctcg gcgggatgat caataccgat gcatccggtc agggatcgct
     2101 ggtctatggc aaaacgtcag atcacgtact tggcgtacgc gcggtgttgt tggggggcga
     2161 tattctcgat acgcaacctt tacccgtcga actggcggaa acgctgggta aaaccaatac
     2221 cacaatcggg cgaatttata acacggttta tcaacgttgc cgtctgcaac gccagttaat
     2281 catcgacaac ttccccaaac ttaaccgctt tcttaccggt tacgatctgc gtcatgtctt
     2341 taacgatgag atgaccgagt tcgacctgac gcgcattctg acgggttcag aagggacgct
     2401 ggcctttatt accgaagcgc ggctggatat tacgcccttg cctaaagtgc gccgtctggt
     2461 gaacgtcaaa tatgactctt ttgactccgc gctgcgtaac gcgccgttta tggttgaggc
     2521 gcgggcgctt tcggtagaga cggtggactc aaaagtgctg aatctggcgc gggaagatat
     2581 tgtctggcat tccgtcagcg agttgattac cgatgtgcct gacaaagaga tgctcgggct
     2641 gaacattgtg gaatttgctg gtgatgatga ggcgctgatt gacgagcgag ttaatgcact
     2701 ctgtgtgcgg cttgatgagc tgattgccag ccaacaggca ggtgtgattg gctggcaggt
     2761 gtgccgcgag ctggcggggg ttgaacgtat ctatgcaatg cgcaaaaaag ccgttggtct
     2821 gcttggcaat gccaaaggtg ccgctaagcc aattccgttt gctgaggata cctgcgtacc
     2881 gccggaacat ctggccgatt atattgctga atttcgcgcg ctgctcgaca gccacggctt
     2941 aagctacggt atgttcggtc acgtcgacgc aggtgttttg cacgtccgtc cggcactgga
     3001 tatgtgcgat ccgcaacaag agattttgat gaagcaaatc tctgatgacg tggtggcgct
     3061 gactgcgaaa tacggtggtt tgttgtgggg cgagcacggc aaaggttttc gcgctgaata
     3121 cagcccggcg tttttcggtg aggaactttt tgcagaactg cgcaaagtga aagcggcatt
     3181 tgacccgcat aaccgactca acccagggaa gatttgcccg ccagaaggtc tcgatgcgcc
     3241 gatgatgaaa gtggacgcgg tgaagcgcgg tacattcgat cggcagatcc ccattgcggt
     3301 acgccagcag tggcgcggtg cgatggagtg taacggcaac ggtttatgct tcaactttga
     3361 tgcccgtagt ccgatgtgtc cgtcgatgaa gatcacccag aaccggattc attcaccgaa
     3421 agggcgcgca acgctggtgc gtgaatggct gcgtttgttg gcggatcgcg gcgttgatcc
     3481 actcaaactg gaacaagaac tgcctgaatc cggcgtcagt ttacggacgt taattgcccg
     3541 cacgcgcaat agctggcatg cgaataaagg cgaatatgac ttctcacacg aagtcaaaga
     3601 ggcgatgtcg ggctgtctgg cctgtaaagc gtgttcgacc cagtgcccca tcaaaattga
     3661 tgtgccggag tttcgctctc gtttcctgca gctctatcac acccgttatt tacgcccgct
     3721 gcgcgaccac ctcgtcgcta cggtcgagag ctacgcaccg ctgatggcac gcgcgccgaa
     3781 aacctttaac ttcttcatta accagccgct ggtgcgcaaa ctctcggaaa aacatatcgg
     3841 catggttgat ttgccactgc tgtcggtccc ctcgctacaa caacaaatgg tggggcatcg
     3901 ctcggcaaac atgacgctgg aacagcttga agctctcaat gcagagcaga aagcgcgcac
     3961 ggtgttggtg gtgcaggacc cctttaccag ctattacgat gcgcaagtgg tggcggattt
     4021 tgtccgtctg gttgaaaaat taggtttcca gcctgtgtta ctgccatttt cgccaaatgg
     4081 caaagcccag catatcaaag gctttcttaa tcgttttgcg aagacggcga aaaagacggc
     4141 ggatttcctc aaccgtatgg ctaagcttgg gatgccaatg gtgggcgtcg atccggcgct
     4201 ggtactttgt tatcgcgatg aatataaact ggcgctgggc gaggaacgtg gcgagtttaa
     4261 cgtcttactt gcgaatgaat ggctggcaag cgcacttgag tcacatccag tggctacagt
     4321 cagcggtgaa tcatggtatt tctttggtca ctgtaccgag gttaccgctt tgccgggcgc
     4381 gccagcacaa tgggccgcga tatttgcccg ttttggcgcg aaactggaaa atgtcagcgt
     4441 gggttgctgc ggcatggcag ggacttacgg acatgaagcg aaaaaccatg aaaattcgct
     4501 cgggatctat gagttatcct ggcatcaggc tatgcagcga ctgccgcgta accgctgtct
     4561 ggcgaccgga tattcctgcc gtagccaggt aaaacgggtt gaaggcacgg gggtacgcca
     4621 tcctgtgcag gctttactgg agattattaa atgatatgga aacggaaaat caccctggaa
     4681 gcactgaatg ctatgggtga aggaaacatg gtggggttcc tggatattcg ctttgaacat
     4741 attggtgatg acacccttga agcgacaatg ccagtagact cgcggacaaa gcagcctttc
     4801 gggttgctgc atggaggagc atccgtggta ctggccgaaa gtatcggttc cgttgccggt
     4861 tatttatgta ccgaaggtga gcaaaaagtg gttggtctgg aaatcaatgc taatcacgtc
     4921 cgctcggcac gagaagggcg ggtgcgcggc atatgtaaac cgttgcatct cggttcgcgt
     4981 caccaggtct ggcagattga aatcttcgat gagaaagggc gtttgtgctg ttcgtcacga
     5041 ttgacgaccg ccattttgtg acgttgtcac cagaaaagtg tgacgcatat tgcttttggt
     5101 cattatctgc acagtaaacg gtggtatact gtgcacgtct tgtctaaaag cgaggatgct
     5161 atgtctactc aacttgatcc cacccagctg gccattgaat ttttacgccg tgaccagagc
     5221 aatctttcac ctgcccaata tctcaaacgt ctgaaacaac tagaacttga atttgctgac
     5281 ctgcttactc tctcttctac tgaattaaaa gaagaaatct actttgcctg gcgtctgggc
     5341 gtgcattgat cctgacttaa tgccgcaagt tcgccaaatc gacctggatt aacctccgtc
     5401 caggttcgat gaccttctta atactgctta tctaccgcct gatattgttc aaaaataata
     5461 ctaatcgcag tttgtgttaa aacggcgggt tagctttatg agattattct tatcgcccct
     5521 tcaagagcta agccactgag agtgccggag ataagcgccg gatggggtag aaacccttaa
     5581 gcctgtgtcg cacagactta agggttttct tatttctgaa cgctgcactt ttttacctat
     5641 ctcttaaata gtcattttca atacaacttt ttctttttca tcacagcctt aatgctcctt
     5701 agataataac cattatctaa caatgagata cctaattctt agcgtgcctg ttaacccaca
     5761 catcagggtc tatgcttatt aaaagcaggc aaaaagcatg ttttacggta aagcccctgc
     5821 gtttgctggg ttgaactgat aatcattatc actagcatgc tgttatacgc tgaaagcgat
     5881 gaagtgaggt aaatcgatgg acatgcattc aggaaccttt aacccacaag atttcgcctg
     5941 gcaaggctta acgctgacac ccgcagcggc gatacacatc cgtgagctgg tggcaaagca
     6001 gccgggtatg gtcggcgtgc gcttaggcgt gaagcaaacg ggctgcgcgg gctttggcta
     6061 tgtgctcgac agtgttagcg agccggacaa agacgatctg ctgtttgaac acgacggcgc
     6121 gaagctgttt gtcccgctgc aagcgatgcc gtttattgat ggcacggaag tcgatttcgt
     6181 tcgtgaagga cttaatcaga tattcaaatt tcacaaccct aaagcccaga atgaatgtgg
     6241 ctgtggcgaa agctttgggg tataggcggt actatgtctc gtaatactga agcaactgac
     6301 gatgtcaaaa cctggaccgg cggcccgctg aattataaag aaggattctt cacccagtta
     6361 gccaccgatg agctggcaaa ggggataaac gaagaggtgg tgcgcgcaat ttcggcgaag
     6421 cgtaatgagc cggagtggat gctggagttt cgtctaaacg cctatcgcgc atggctggag
     6481 atggaagaac cgcactggtt gaaagcgcac tacgacaagc tgaattatca ggattacagt
     6541 tactactcag caccatcgtg cggtaattgt gacgacactt gcgcgtctga acctggcgcg
     6601 gtgcagcaaa ctggcgcgaa cgccttttta agtaaagagg tggaggcggc gtttgagcag
     6661 ttgggcgttc ccgtgcggga aggcaaagag gtggcggtgg atgccatttt cgactctgtt
     6721 tcggttgcca ctacttatcg cgaaaaactg gcggagcagg gaattatttt ctgttccttt
     6781 ggtgaggcga tccacgatca tccggaactg gtgcgtaaat atctcggcac tgtggtgccg
     6841 ggaaatgaca acttctttgc cgcgcttaat gcggcggtag cctctgacgg tacgtttatt
     6901 tatgtgccta aaggcgtgcg ctgcccgatg gaactttcca cctattttcg cattaacgcg
     6961 gaaaaaaccg ggcagtttga gcgcaccatt ctggtggccg acgaagacag ctacgtcagc
     7021 tacattgaag gctgttccgc tccggtgcgt gacagctatc agttacacgc ggcggtggtg
     7081 gaagtcatca tccataaaaa cgccgaggtg aaatattcca cggtacaaaa ctggttccct
     7141 ggcgataaca acaccggcgg tattctcaat ttcgtcacca agcgtgcttt gtgcgaaggc
     7201 gaaaacagca aaatgtcatg gacgcaatca gaaaccgggt cagcgattac gtggaaatat
     7261 cccagctgca ttttgcgcgg cgataactcc attggtgagt tttactcagt ggcgctgacc
     7321 agcggtcatc agcaagcgga taccggcacc aagatgatcc acatcggtaa aaacaccaaa
     7381 tcgaccatta tctcgaaagg gatctctgcc ggacatagtc agaacagtta tcgcggctta
     7441 gtgaaaatca tgccgacggc aaccaatgcg cgcaatttca ctcagtgcga ctcaatgctg
     7501 attggcgcta attgtggggc gcataccttc ccgtatgtcg aatgtcgcaa taacagcgca
     7561 caactggagc acgaggcaac gacatcacgt attggtgaag atcaactgtt ttactgcctg
     7621 caacgcggga ttagcgaaga agacgccatc tcgatgattg ttaacggttt ctgcaaagac
     7681 gtgttctcag aactgccgct ggaatttgcc gttgaagcac aaaaactcct cgccatcagt
     7741 cttgaacaca gcgtcggata aggaataaac atgttaagta ttaaagattt acacgtcagc
     7801 gtggaagata aagctatcct gcgtggatta agcctcgacg ttcgtcccgg cgaagttcac
     7861 gccattatgg ggccaaacgg ttcgggcaaa agtaccttat cggcaacgct tgccgggcga
     7921 gaagattatg aagtgacggg cggcacggtt gagttcaaag gcaaagattt gcttgcgctg
     7981 tcgccggaag atcgtgcagg cgaaggcatc tttatggcct tccagtatcc ggtggagatt
     8041 cccggcgtca gtaaccagtt tttcctgcaa acggcactta atgcggtgcg cagctatcgc
     8101 ggccaggaaa cgctcgaccg ctttgatttt caggatttga tggaagagaa aatcgctctc
     8161 ctgaagatgc cggaagattt attaacccgt tcggtaaacg ttggtttttc cggcggcgag
     8221 aaaaagcgca acgatatttt gcaaatggcg gtgctggaac cggagttatg cattcttgat
     8281 gaatcggact ccgggctgga tattgacgca ttaaaagtgg ttgccgatgg cgtgaactcg
     8341 ctgcgtgacg gcaagcgctc attcatcatt gttacgcact accaacgcat tctcgactac
     8401 atcaagcctg attacgtcca tgtgctatat cagggacgaa ttgtgaaatc cggcgatttc
     8461 acgttggtca aacaactgga ggagcagggt tatggctggc ttaccgaaca gcagtaatgc
     8521 gctgcaacag tggcatcact tgtttgaagc tgaagggaca aaacgctcac cgcaagcaca
     8581 gcagcattta caacaattgc tgcgtaccgg actgccgaca cgtaaacatg aaaactggaa
     8641 atatacgccg ctggaagggc tgaccaatag tcagtttgtc agcattgcgg gagatatatc
     8701 cccacagcag cgtgatgcct tagcgttaac gttagacgcc gtgcggctgg tgtttgtcga
     8761 tggacgttac gtgcccgcgc tgagcgatgc aattgaaggc agcggatatg aagtgagcat
     8821 taacgacgac cgtcaggggt tacccgacgc tattcaggcg gaagtgtttc tgcatttgac
     8881 ggaaagcctg gcacaaagcg tgacgcatat cgccgtgaag cgcggtcaac ggccggcaaa
     8941 gccattgctg ttaatgcata tcacccaggg cgtggcaggt gaagaggtga acactgccca
     9001 ttaccggcat catctggatc tggcggaagg tgccgaagca acggtgatcg aacattttgt
     9061 cagccttaat gatgctcgtc attttaccgg ggcacggttc actatcaatg tcgcagcgaa
     9121 cgcccacttg cagcatatca agctggcgtt tgaaaacccg ctcagtcacc actttgctca
     9181 taacgatttg ttgctggctg aggatgccac cgcatttagc catagtttcc tgctgggtgg
     9241 cgcagtgtta cgacacaaca ccagtacgca actcaatggc gaaaacagca cgctgcggat
     9301 caatagcctg gcgatgccgg tgaaaaacga ggtgtgtgat acccgtacct ggctggaaca
     9361 caataaaggt ttttgtaaca gccgacagtt gcacaaaact atcgtcagcg acaaaggccg
     9421 cgcggtattt aacggtttga tcaacgtcgc gcagcacgcc atcaaaacgg atggtcagat
     9481 gaccaacaat aatctgctga tgggcaaaat ggcggaagtg gatacaaaac cgcagctgga
     9541 aatctatgca gatgatgtga aatgcagcca cggcgcgacg gtggggcgta ttgatgatga
     9601 acagatgttc tatctgcgct cgcgcgggat caatcagcag gatgcccagc agatgatcat
     9661 ttacgccttt gctgccgaac tgacggaagc actgcgtgat gaggggctta aacagcaggt
     9721 gctggcccga atcggtcaac ggctgccagg aggtgcaaga tgactttttc cgtcgacaaa
     9781 gtgcgggccg actttccggt gctttctcgt gaggtgaacg gtttgccgct ggcttatctc
     9841 gatagcgccg ccagtgcgca gaaaccaagc caggtgattg acgccgaggc tgagttttat
     9901 cgtcacggct acgcggcggt gcatcgcggt attcatacct taagcgccca ggcgaccgag
     9961 aaaatggaga acgtgcgcaa gcgggcatcg ctgtttatta atgcccgttc ggcggaagag
    10021 ctggtgttcg tccgcggcac gacggaaggg atcaatctgg tcgccaatag ttggggcaac
    10081 agcaacgtgc gggcgggcga taacatcatc atcagtcaga tggagcacca cgctaacatt
    10141 gttccctggc agatgctttg cgcacgcgtt ggcgcagagc tgcgtgtgat ccctctcaac
    10201 cccgacggta cgctgcaact ggagacgctg cctacgctgt ttgatgagaa aactcgcctg
    10261 ctggcaatta ctcatgtctc caacgtgctg ggaacagaaa atccgctggc ggaaataatc
    10321 acgcttgcgc accagcatgg cgcaaaagtg ctggtggatg gcgctcaggc ggtgatgcat
    10381 catccggtgg atgttcaggc gctggattgc gacttttacg tgttctccgg gcataaactg
    10441 tatggcccca ccggaattgg cattctttat gtgaaagaag ccttgttgca ggagatgccg
    10501 ccgtgggaag ggggcggttc tatgatcgcc accgtcagcc tgagtgaagg cactacctgg
    10561 accaaagcac catggcggtt tgaagccggt acacccaata ccgggggcat tattggtctt
    10621 ggcgcggcgc tggagtatgt ttcggcgctg gggcttaata acatagccga gtatgaacag
    10681 aatctgatgc attatgcgct atcacagctg gaatctgtac cggatctcac tctctatggc
    10741 ccacaaaaca ggcttggcgt tattgctttt aatctcggta aacaccacgc ctatgatgtt
    10801 ggcagttttc tcgataatta cggcattgct gtgcgtaccg gacatcactg cgctatgcca
    10861 ttaatggcct attacaacgt ccctgcgatg tgtcgggcgt cgctggccat gtataacacc
    10921 catgaagaag tggatcgtct ggtgaccggc ctgcaacgta ttcaccgttt gctgggataa
    10981 cagggaggca ctatggcttt attgccggat aaagaaaaat tgctgcgtaa ttttttacgc
    11041 tgcgccaact gggaagagaa atatctctac attattgagc tgggccagcg tctgactgaa
    11101 ttacgcgacg aagaccgaag cccacaaaat agcattcagg gctgccagag tcaggtgtgg
    11161 attgtcatgc gccagaatgc gcacggaatt attgaattgc agggcgacag cgatgcggcg
    11221 attgtgaaag ggcttattgc ggtagtcttt attctctacg atcagatgac gccgcaggat
    11281 attgtcagtt tcgatgtgcg tccgtggttt gaaaaaatgg cactcaccca acatctcacc
    11341 ccatctcgtt cacaaggtct ggaagcgatg attcgcgcaa ttcgcgccaa agccgctgca
    11401 cttagctaaa ctaaagggac agctttcatc cggttggctc acattgccgg atggtcaatg
    11461 tttacactga cctgcacagg agtgttttgg tttcaggtga acataagcgc catccgacgt
    11521 ttcagcgtga gtctccggca aatacaggag gtttacaatg aaacgcgcgt ctttgcttac
    11581 actcacgctt atcggcgctt ttagcgccat ccaggctgcc tgggcggttg attatccgct
    11641 accaccaacc ggaagccgac tggttgggca aaatcaaacg tatacggtgc aagaagggga
    11701 taaaaacctt caggctattg cccgacgttt tgatactgcg gcaatgttga tccttgaagc
    11761 caataacact atcgtcccgg tgccaaaacc tggtacgacg ataactattc cttcacaact
    11821 gttattacct gatgcaccgc gtcaggggat tatcgttaac cttgcagagc tgcgccttta
    11881 ttattatccg ccgggagaaa atattgtgca ggtttatcca ataggtattg gattgcaggg
    11941 gctggaaacg ccggtgatgg aaacgcgtgt tgggcagaaa atccctaacc caacctggac
    12001 gcctacggca ggcattcgtc agcgttcgct ggagcgtggc attaaattac cgccagtcgt
    12061 tcctgccgga ccaaataacc cgctaggacg ttacgcactg cgcctcgcgc atggtaatgg
    12121 cgaatacctc attcatggca ccagtgcgcc ggacagcgtt ggtttgcgcg tcagttcagg
    12181 gtgtattcgc atgaatgcac cggatattaa agccttgttc tccagcgtgc ggacgggaac
    12241 gccggtgaaa gtgatcaacg aaccggtgaa atattccgtg gaacctaacg ggatgcgtta
    12301 tgttgaagta catcgaccac tatcagcaga agaacagcaa aacgttcaga caatgccata
    12361 cacactgcca gcaggcttta cgcaatttaa agacaataag gctgtagatc agaagttagt
    12421 cgataaagcg ttgtatcgtc gggcagggta tccggtttcg gtgagcagtg gagcaactcc
    12481 cgcagccagc aatgcgcctt cagtagagtc agcgcagaat ggtgaaccag agcaagggaa
    12541 tatgttacgc gcgacgcagt agtggtaaat agcagacaaa aaaatggcgc acaatgtgcg
    12601 ccatttttca cttcacaggt actattactt gcggtattta gtagccatgt tgtccagacg
    12661 ctggttagca cgagctgcgt catctttagc agcctgaacg tcggaacgca ttgcgttcac
    12721 gtcgttgctc agctggtcaa ctttagcgtt cagagtctga acgtcagaag acagctgatc
    12781 gattttagcg ttgctggagc aacctgccag cagagtagaa cccaggatta ccgcgcccag
    12841 taccagttta gtagctttca ttattaatac cctctagatt gagttaatct ccatgtagcg
    12901 ttacaagtat tacacaaagt tttttatgtt gagaatattt ttttgatggg aaggcactta
    12961 tttttgatcg ttcgctcaaa gaagcatcga acgctgggtt ttagataaaa aaactgagag
    13021 aaaacaggat gcttccatcg gattcatctt agataaagtg agattatata gtgaaatccg
    13081 atttgatttt ttattgcttc tggttatcga ttaaataaaa aagcgcccat cagggcgctt
    13141 cgatatacaa attaattcac aaaagcaata ttacaggacg tgaacagatg cggtgttagt
    13201 agtgccgctc ggtaccagtg caccagaaac cataactacg acgtcacctt tgtgtgccag
    13261 accgctctgc agagccagtt ctttacccag acggtagaaa tcatcagtag aagtgatctc
    13321 tttaaccagc tgcggcacaa cgcctttgct cagtaccaac tgatgagccg ttttttcgtt
    13381 ggtggtcagt gccagtatgg tggcatccgg gaagtattta cgtactgcgc gagcagattt
    13441 accgccctgg gtagcaacca cgatcagcgg agcatccagt ttttcagcag tttcaacggc
    13501 accacggcat accgcttcgg taatgcgcag tttacggttg tcattgttga actcgagacg
    13561 gctgttcatc acgcggtcgg tacgttcgca gatggtcgcc atgatagaaa ccgcttccag
    13621 cgggtattta ccttttgcgg attcaccaga cagcatcact gcgtcagtac cgtcgaggat
    13681 ggcgtttgca acgtcaccgg cttctgcgcg agtcgggcgt gggtttttga tcatggaatc
    13741 cagcatctgg gtcgcagtga taacgacttt acgtgcacgg atacattttt cgatcatcat
    13801 cttctgggcg aagataactt cttctaccgg aatttctaca cccaggtcgc cacgcgcaac
    13861 catgatgccg tcagaggctt cgaggatttc gtcgaagttg ttgaggcctt cctggttttc
    13921 gattttggag atgatgtgga tgttttcgcc gccgtgcgct ttcaggtgct cacggatttc
    13981 gataacgtca gaacgcttac gaataaagga agcagcaaca aagtctacgc cttgttcgca
    14041 accaaagatc aggtcctgtt tgtctttttc agccagtgct ggcagggcaa tggaaacgcc
    14101 aggcaggttc acacctttgt tttcgcccag gtcgccgttg ttcagcactt tacagataac
    14161 tttgttacct tcaatggcag taacttccat accgatcaga ccatcgtcaa ccagtacggt
    14221 gttgccaaca gacaggtcag tagtgaaacc ttcatacgtt accgcaacca tttcgctgtt
    14281 gccgataaca gatttatcag tggtgaaagt aaaggtctga cccgctttca gagaaacgtc
    14341 gttaccgcct tccagtttca tggtgcggat ttccggacct ttggtatcaa gcaggatagc
    14401 ggcggtttta ccagttttgc tcatcacgtt gcgcagattc tgaatgcgct gaccgtgttc
    14461 tgcatagtca ccatgagaga agttcagacg cataacgttc atgccagcgt ccagcatttt
    14521 agctaacatc tcttcagatt cggttttcgg tccgatggtg caaacaattt tggtcttttt
    14581 catgacagtc ttagtcttta agttgagaag gatgggagaa acttgctttc tgggcgcact
    14641 gccggaaagc gaatccggta ttacgaaagt ggtggtgcct tgctctaagg ataggtgaca
    14701 tcgaaaaagc gtgcagagga atgtgtgctt gtgtctcagc ccaacgtgag tgcctgtttt
    14761 tgagtcgttg agttctacag tccaaagtgc tgaaaccatt caagagtcaa ttggcgcgca
    14821 ttatacgctg aaattaatcc aaaaggaaaa caaaaacagc gtttcaaaaa atatacaact
    14881 tgttagacaa taaattgtta tcaaagattt aacgttccag ggaaaaggtt acgcaaaaac
    14941 ttcgctgttt gcgcaacgtc tggtgccgaa gttgtcagtg tgaggataag tgaaagttca
    15001 tccggtgtat aaatatatca tcgctcaatt gctacagtta aaataattta tcgcgttatt
    15061 tgtctgattt tgtttgattt ttgtttaatt ggttagattg atatatgctg atagttggca
    15121 tgataaatag gtataaagaa tgggaaatcg gacaaaagag gatgaactgt accgagaaat
    15181 gtgcagagtt gtcggtaaag ttgtgctgga aatgcgcgat ctggggcagg aaccaaagca
    15241 tattgttatc gcaggcgtat tacgtactgc attagccaat aaacgcattc agcgcagtga
    15301 gctggaaaag caggcgatgg aaaccgtcat taacgcactg gtaaaatgac cattcaggat
    15361 gaggcggtaa gcttcatcct gatattcttg cttcagttgc ggtccagtta atcattctaa
    15421 ataacaactg ctgaaattat acgaattgcg gtatgtattt taatctataa cgataatatt
    15481 atttttattt tctgcaatga acgtcacaaa taattgtcgc ttaagt
//
LOCUS       DAEEKQ010000089        15514 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.089, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000089 DAEEKQ010000000
VERSION     DAEEKQ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15514
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.089"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..1114
                     /locus_tag="KLK57_002642"
     CDS             <1..1114
                     /locus_tag="KLK57_002642"
                     /EC_number="4.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBE0892972.1"
                     /translation="DLRCVNMVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWR
                     WRKRMEAAGKPTDKPNLVCGPVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEAC
                     DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHIDAASGGFLAPFVAP
                     DIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFA
                     INFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICAGRPD
                     EGIPAVCFKLKEGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFE
                     MDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            1270..2805
                     /gene="gadC"
                     /locus_tag="KLK57_002643"
     CDS             1270..2805
                     /gene="gadC"
                     /locus_tag="KLK57_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HBE0892973.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2936..4255
                     /locus_tag="KLK57_002644"
     CDS             2936..4255
                     /locus_tag="KLK57_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HBE0892974.1"
                     /translation="MDICSRNEKLAIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4627..6009
                     /gene="dosC"
                     /locus_tag="KLK57_002645"
     CDS             4627..6009
                     /gene="dosC"
                     /locus_tag="KLK57_002645"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HBE0892975.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIAVAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKILLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVHSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            6034..8433
                     /gene="dosP"
                     /locus_tag="KLK57_002646"
                     /gene_synonym="pdeO"
     CDS             6034..8433
                     /gene="dosP"
                     /locus_tag="KLK57_002646"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HBE0892976.1"
                     /translation="MKLTDAENAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMQAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8692..9273
                     /gene="ddpX"
                     /locus_tag="KLK57_002647"
     CDS             8692..9273
                     /gene="ddpX"
                     /locus_tag="KLK57_002647"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HBE0892977.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFSCFISPGTQHVS"
     gene            9287..10837
                     /locus_tag="KLK57_002648"
     CDS             9287..10837
                     /locus_tag="KLK57_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE0892978.1"
                     /translation="MKRSISFRPTLLALVLATTFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDTPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSGQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10839..11861
                     /locus_tag="KLK57_002649"
     CDS             10839..11861
                     /locus_tag="KLK57_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0892979.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLAGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYFSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11858..12754
                     /locus_tag="KLK57_002650"
     CDS             11858..12754
                     /locus_tag="KLK57_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HBE0892980.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLISWHILRNSLPPLIVPA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12751..13737
                     /locus_tag="KLK57_002651"
     CDS             12751..13737
                     /locus_tag="KLK57_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0892981.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGYVHVLNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGDDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAITLLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGVTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISI"
     gene            13766..14656
                     /locus_tag="KLK57_002652"
     CDS             13766..14656
                     /locus_tag="KLK57_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0892982.1"
                     /translation="MNFPARKSWLGKTTEHVHAINGIDLQIRRGETLGIVGESGCGKS
                     TLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLSVWRIITEPLWIAKRSS
                     EQQRRALAKELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQPDVIVLDEPTSA
                     LDISVQAQILNLLVTLQENHGLTYVLISHNVSVIRHMSDRVAVMYLGQIVELGDAQQV
                     LTAPAHPYTRLLLDSLPAIDKPLEEEWALCKTDLPGNRTLPQGCFFYERCPLATHGCE
                     VRQSLAIREDGRELRCWRAL"
     gene            complement(14712..15143)
                     /gene="osmC"
                     /locus_tag="KLK57_002653"
     CDS             complement(14712..15143)
                     /gene="osmC"
                     /locus_tag="KLK57_002653"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HBE0892983.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTQSGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDADFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3809 a         3867 c         3949 g         3889 t
ORIGIN      
        1 cgacctgcgt tgcgtaaata tggttgccga tctgtggcat gcgcctgcgc cgaaaaatgg
       61 tcaggccgtt ggcaccaaca ccattggttc ttccgaggcc tgtatgctcg gcgggatggc
      121 gatgaaatgg cgttggcgca agcgtatgga agctgcaggc aaaccaacgg ataaaccaaa
      181 cctggtgtgc ggtccggtac aaatctgctg gcataaattc gcccgctact gggatgtgga
      241 gctgcgtgag atccctatgc gccccggtca gttgtttatg gacccgaaac gcatgattga
      301 agcctgcgac gaaaatacca tcggcgtggt gccgactttc ggcgtgacct acaccggtaa
      361 ctatgagttc ccgcaaccgc tgcacgatgc actggataaa ttccaggccg acaccggtat
      421 cgacatcgac atgcacatcg acgccgccag cggtggcttc ctggcaccgt tcgtcgcccc
      481 ggatatcgtc tgggacttcc gcctgccgcg tgtgaaatcg atcagtgctt caggccacaa
      541 attcggtctg gctccgctgg gctgcggctg ggttatctgg cgtgacgaag aagcgctgcc
      601 gcaggaactg gtgttcaacg ttgactacct cggtggtcag attggtactt ttgccatcaa
      661 cttctcccgc ccggcgggtc aggtgattgc acagtactat gaattcctgc gccttggtcg
      721 tgaaggctat accaaagtac agaatgcttc ctaccaggtc gctgcctatc tggcggatga
      781 gatcgccaaa ctggggccgt atgagttcat ctgtgccggt cgcccggacg aaggcatccc
      841 ggcggtttgc ttcaaactga aagagggtga agatccggga tacaccctgt acgacctctc
      901 tgaacgtctg cgtctgcgcg gctggcaggt tccggccttc actctcggcg gtgaagccac
      961 cgacatcgtg gtgatgcgca ttatgtgtcg tcgcggcttc gaaatggact ttgctgaact
     1021 gttgctggaa gactacaaag cctccctgaa atatctcagc gatcacccga aactgcaggg
     1081 tattgcccag cagaacagct ttaaacatac ctgataacgt ttaacggtaa cggtgtcccg
     1141 aaacgaaccc gtttcgggat aatttccaaa gtctgttcac tggcattagc aacggaaaat
     1201 attgttctga atacgcttca gaacaaaaca ggtgcggttc cgacaggaat accgttttag
     1261 ggggataata tggctacatc agtacagaca ggtaaagcta agcagctcac attactcggc
     1321 ttttttgcca taacggcatc gatggtaatg gctgtttatg aataccctac cttcgcaaca
     1381 tcgggctttt cattagtctt cttcctgcta ttaggcggga ttttatggtt tattcccgtg
     1441 ggactttgtg ctgcggaaat ggccaccgtc gacggctggg aagaaggtgg tgtcttcgcc
     1501 tgggtatcaa atactctggg gccgagatgg ggatttgcag cgatctcatt tggctatctg
     1561 caaatcgcca ttggttttat tccgatgctc tatttcgtgt taggggcact ctcctacatc
     1621 ctgaaatggc cagcgctgaa tgaagacccc attaccaaaa ctattgcagc actcatcatt
     1681 ctttgggcgc tggcattaac gcagtttggt ggcacgaaat acacggcacg aattgctaaa
     1741 gttggcttct tcgccggtat cctgttacct gcatttattt tgatcgcatt agcggctatt
     1801 tatctgcact ccggtgcccc cgttgctatc gaaatggatt caaagacctt cttccctgac
     1861 ttctctaaag tgggcaccct ggttgtattt gttgccttca ttttgagtta tatgggcgta
     1921 gaagcatccg caacccacgt caatgaaatg agcaacccag ggcgcgacta tccgttggct
     1981 atgttactgc tgatggtagc ggcaatctgc ttaagctctg ttggtggttt gtctattgcg
     2041 atggtcattc cgggtaatga aatcaacctc tccgcagggg taatgcaaac ctttaccgtt
     2101 ctgatgtccc atgtggcacc ggaaattgag tggacggttc gcgtgatctc cgcactgctg
     2161 ttgctgggtg ttctggcgga aatcgcctcc tggattgttg gtccttctcg cgggatgtat
     2221 gtcacagcgc agaaaaacct gctgccagcg gcattcgcta aaatgaacaa aaatggcgta
     2281 ccggtaacgc tggtcatttc acagctggtg attacttcta tcgcgttgat catcctcacc
     2341 aataccggtg gcggtaacaa catgtccttc ctgatcgcac tggcgctgac ggtggtgatt
     2401 tatctgtgtg cttatttcat gctgtttatt ggctacattg tgttggttct taaacatcct
     2461 gacttaaaac gcacatttaa tatccctggt ggtaaagggg tgaaactggt cgtggctatt
     2521 gtcggtctgc tgacttcaat tatggcgttt attgtttcct tcctgccgcc ggataacatc
     2581 cagggtgact ctaccgatat gtatgttgaa ttactggtcg ttagtttcct ggtggtactt
     2641 gccctgccct ttattctcta tgctgttcat gatcgtaaag gcaaagcgaa taccggcgtc
     2701 actctggagc caatcaacag tcagaacgca ccaaaaggtc acttcttcct gcacccgcgt
     2761 gcacgttcac cacactatat tgtgatgaat gacaagaaac actaaccatg tctgaatggt
     2821 tataagacaa gggagcgata attcatcgct ccctttttcg tgctcgctgt cgtattgact
     2881 taaccgggat aagtacgaga atgagcgcac atctgtttac cggaaaccag catatatgga
     2941 tatctgctcc cgaaacgaga aattagcgat tagaagacca gcgatactgg ttgcactggc
     3001 acttttactg tgtagttgta aaagcacgcc tccagagtcc atggtgacac caccagcagg
     3061 ttcaaagcca ccagccacga cgcaacaatc gtcacaaccg atgcgtggca tctggctggc
     3121 cacggtgtct cgcctcgact ggccaccggt ttcctcggtt aacattagta accccaccag
     3181 ccgggcccgt gtacaacaac aggcgatgat cgacaaactg gatcatctgc aacgtctcgg
     3241 tataaacacg gtctttttcc aggttaagcc ggacggtacc gccctgtggc catcgaaaat
     3301 tttgccatgg tccgatctta tgaccggtaa gattggtgaa aatccgggtt acgatccgct
     3361 gcaatttatg ctcgacgaag cccacaagcg tgggatgaaa gtacacgcct ggtttaaccc
     3421 ctatcgcgta tcggttaata cgaagcccgg tactatcagg gaactgaata gcactctgtc
     3481 tcaacaaccg gcgagcgtct atgtgcaaca ccgtgactgg atcagaacgt ctggcgatcg
     3541 ctttgtcctc gacccgggca tccctgaggt tcaggactgg atcacatcaa tagtcgcaga
     3601 agtggtttcc cgctatccgg tagatggcgt gcagtttgac gactatttct atactgagtc
     3661 accgggttca cggctaaatg ataacgaaac gtaccgcaaa tacggaggcg catttgcgtc
     3721 aaaagcagac tggcggcgca acaatactca gcagttaatt gcaaaagtat cgcacaccat
     3781 taaaagcatt aagccgggag tcgaatttgg cgttagcccg gcaggcgtgt ggcgtaaccg
     3841 atcacacgat ccgctcggtt ccgatacccg aggcgcggca gcctatgacg aatcctacgc
     3901 tgacacccgt cgatgggtgg aacaaggatt gctggattac attgctcccc aaatttactg
     3961 gccgttctca cggagtgccg cgcgttatga cgtgttggca aaatggtggg cggatgtcgt
     4021 taaaccgact aggacccgcc tgtatatcgg tatcgccttc tataaagtgg gtgaaccttc
     4081 aaagatagag ccagactgga tgattaacgg cggcgtaccg gaactgaaaa agcagctcga
     4141 tcttaacgat gcggtaccag aaattagcgg caccatcttg ttccgtgagg actatctgaa
     4201 taaaccgcag actcaacaag cggtcagcta tctgcaaagt cgttggggca gttaaactcc
     4261 catgcttact ttgtaacaag ccggtgcttt acccaccggc ttgttacacc ttgtgaaata
     4321 tcacactagt aatcatgctt acataagtca aattaaccac gcaattaacc acacttagtt
     4381 aaacagctat aaatccctaa taaaaatgta tgaatatgaa aaaccatacg atattcgctg
     4441 tcaccgaaaa ataattacga tgggctatac tcttctgaag aacagtagcg tagattgaaa
     4501 ctgtcctaag tcgcttattt cttttcagta tatcttcata tttcaggaga atatggtgca
     4561 gttagtcaat ctcgcacgtt gcgttttgat tccgtctgat ttcccagcct tataagggtg
     4621 tgaattatgg agatgtattt taaaagaatg aaagatgagt ggaccggact tgtcgaacag
     4681 gcagatccac tcattcgtgc taaagccgcg gaaattgccg ttgcgcatgc tcattatctg
     4741 agtatcgagt tttatcgaat tgtccgcatc gacccgcatg ccgaagaatt cttgagtaat
     4801 gaacaagttg agcggcagtt gaaaagtgcg atggaacgct ggattattaa cgtgctttct
     4861 gcccaggttg acgatgtcga aaggctaata caaatccagc ataccgtcgc ggaagtgcat
     4921 gcccgcatag gaattccggt agaaattgtc gagatggggt ttcgggtgct gaaaaaaatc
     4981 ctctatccgg taatcttctc ttcggattat tccgccgcag aaaaacttca ggtctatcat
     5041 ttctcgatta acagtattga tatcgcgatg gaagtgatga cccgcgcgtt tacctttagt
     5101 gacagtagtg cctcaaagga agatgaaaat tatcgtatct tctcgttact ggaaaacgcc
     5161 gaagaagaaa aagaacggca aatagcctca atactttcat gggaaataga tattatctat
     5221 aaaatcctgc tggattctga tttaggcagt agtttgccat taagccaggc tgattttggc
     5281 ctgtggttta accataaagg tcgacattat tttagtggca ttgccgaagt aggccatatc
     5341 tcccgtctca ttcaggattt cgacggtatt ttcaatcaaa ccatgcgtaa caccaggaat
     5401 ttgaataaca gaagtctgcg ggtgaaattt ttattacaga taagaaatac cgtatcgcag
     5461 attattacct tgctgcgtga attgtttgaa gaagtatcgc gccacgaagt cggtatggat
     5521 gtactaacga aattacttaa ccgccgtttc ctgccgacta tcttcaaacg cgaaattgcc
     5581 catgccaacc ggaccggtac accgctgtca gtgctgatta ttgacgttga taaattcaaa
     5641 gagatcaacg atacgtgggg ccataacact ggcgatgaaa ttctgcgtaa agtctctcag
     5701 gccttttatg acaacgtcca cagtagtgat tatgtttttc gctacggggg cgatgaattt
     5761 atcattgttt tgactgaagc ttctgaaaac gaaacgttac gtaccgcaga acgtattcgc
     5821 agtcgggtgg agaaaaccaa actgaaagcc gcaaacggcg aggatattgc cctctcactt
     5881 tccatcggtg ccgccatgtt taatggtcat cctgactatg agcgcctcat tcaaatagcc
     5941 gatgaagctc tgtatatcgc caaaagacga ggtagaaacc gtgttgaact ctggaaagcc
     6001 agtctttaga tgcgccagga tgcagaggta atcatgaagc taaccgatgc ggaaaatgcc
     6061 gccgatggca tttttttccc cgcccttgaa caaaatatga tgggcgcggt gttaattaac
     6121 gaaaatgatg aagtgatgtt tttcaacccc gccgcagaga agctctgggg gtacaaacgt
     6181 gaagaagtca ttggcaataa cattgatatg ctgattccgc gggatttgcg tcctgcgcat
     6241 cctgaataca ttcgtcataa ccgtgaaggt ggtaaagcgc gcgtcgaggg gatgagtcgg
     6301 gagctgcagc tggagaaaaa agacggcagt aaaatctgga cccgttttgc gctatcgaaa
     6361 gtgagcgccg aggggaaagt ttattacctg gcgctggtac gggatgccag cgtagaaatg
     6421 gcgcaaaaag aacagacccg acaattgatt attgccgttg accatctcga ccgaccggtg
     6481 attgtcctcg atccggaacg ccatattgtg cagtgcaatc gcgcatttac cgaaatgttt
     6541 ggttactgca ttagcgaagc cagcggtatg cagcccgata cactcctgaa cattcctgaa
     6601 ttccctgccg ataaccgcat tcgtttacaa cagttgctat ggaaaaccgc ccgcgatcag
     6661 gacgaatttc tgctgttgac acgcaccggt gaaaaaatct ggattaaagc ctctatcagc
     6721 ccggtttatg acgtgctcgc gcatctgcag aacctggtaa tgaccttctc ggatatcacc
     6781 gaagaacggc agatccgcca gcttgaaggc aatattctcg ccgccatgtg cagcagcccg
     6841 ccatttcatg aaatggggga aatcatttgt cgtaacatcg aatctgttct caacgaatcg
     6901 catgtttcgc tgttcgcact gcgcaacggg atgccgatac actgggcgtc atcttcgcac
     6961 ggtgcagaag ttcaaaatgc gcaaagctgg tcagcgacca ttcgtcagcg tgatggtgcg
     7021 cctgcgggga tcctgcaaat taaaacctcg tcaggagcag aaaccagcgc ctttatcgaa
     7081 cgcgtggcag atatcagcca gcatatggcc gcgctggcgc tggaacagga aaaaagccgt
     7141 cagcatattg aacaactcat ccaatttgat ccgatgaccg gtctgccaaa tcgcaataac
     7201 ctgcacaatt acctcgatga ccttgtcgac aaagccgtct ctccggtggt gtatctcatc
     7261 ggtgttgacc atattcagga tgtgattgat agccttggct atgcgtgggc cgatcaggca
     7321 ttgctggaag tggtcaatcg ctttcgtgaa aaactcaaac cggatcagta tctctgtcgt
     7381 atcgaaggta cgcagtttgt cctcgtgagc ctggaaaacg acgtcagtaa cattacccaa
     7441 atcgccgatg agctacggaa tgtggtcagc aagccgataa tgattgacga taaaccattc
     7501 ccgctaacct tgagtattgg catcagctac gacgtgggta aaaaccgcga ttacctgctc
     7561 tccactgctc acaatgcaat ggattatatt cgcaagaatg gcggtaacgg ctggcagttc
     7621 ttcagcccgg cgatgaacga aatggtaaaa gagcgtttgg ttttaggcgc agcgctgaaa
     7681 gaagcgatta gcaataacca actgaaactg gtttaccagc cgcaaatctt cgcagaaacg
     7741 ggtgaactgt acggcatcga agcccttgct cgctggcacg atccccagca tggtcatgta
     7801 cccccttcac ggtttattcc tctcgcagaa gagattggtg aaatcgaaaa tattgggcgc
     7861 tgggtcatcg cggaagcttg ccgtcagtta gcagaatggc gtagccagaa tattcatatc
     7921 ccggcgttat ccgtgaactt gtcggcgctg cactttcgca gtaatcagct gcctaatcag
     7981 gtgtctgatg caatgcaagc ctggggtatt gacggccacc agctgacggt agaaatcacg
     8041 gaaagcatga tgatggaaca cgataccgaa atctttaagc gcattcagat cctgcgcgat
     8101 atgggcgtgg gcttatcggt agatgatttt ggcacgggct tttccggatt atcccgctta
     8161 gtcagtcttc cggtaacgga aatcaaaatt gacaaaagtt ttgtcgatcg ttgtctgacg
     8221 gaaaaacgca tccttgcctt acttgaagcc attaccagca ttgggcaaag cctcaattta
     8281 accgtcgtgg cggaaggcgt cgaaaccaaa gagcaatttg agatgctacg caagatccac
     8341 tgtcgcgtta ttcagggata tttcttttcc cgccccctac ccgccgagga aattccaggc
     8401 tggatgagca gcgtgttacc gctgaaaatc tgacaaattc ctctcgcccg cactcgcggg
     8461 ttttcattta acgtgacgct gtcacttaaa tactgtgatt tcagctacga ttccgggaga
     8521 ttccttcctt aacttcccct cttgcaccaa aactgcacta cgctgcacaa atgttgatca
     8581 aaagttaaaa aatataactt taagccagca ttctggcgct tatcccggca tggcatgaga
     8641 tctgcataag cggaaagcgc agcaattttt gtcttaatac aggaaccgtt tatgtcggat
     8701 accaccgaac tggttgattt agccgtgatc ttccctgatc tggagatcga attgaaatac
     8761 gcctgcgctg ataacatcac aggtaaagct atttaccagc aagcgcgttg tctgttacac
     8821 aaggatgcga ttaccgcgct ggcgaaaagt atcagtatcg cccagctgtc aggcttacaa
     8881 ctggtgattt acgatgcgta tcgcccacaa caagcacagg cgatgttgtg gcaagcctgc
     8941 ccagacccgc aatatgttgt tgatgtgacg gtcggttcta atcacagccg tggcacggcg
     9001 atcgacctga cgcttcgtga tgagcacggg aacatcctgg atatgggcgc agggttcgat
     9061 gaaatgcacg agcgctccca tgcctatcac ccttccgtcc cgcccgccgc tcagcgcaat
     9121 cgcctgttgc tgaatgcgat aatgactggt ggcggttttg tcggtatctc cagcgaatgg
     9181 tggcacttcg aattacccca ggcagcgagt taccctctgc tcgctgatca attctcttgt
     9241 tttatatccc ctggcacaca gcacgtcagt taatcaggaa actgtcatga agagatcgat
     9301 atcgtttcgt cccacattgc tcgcgctcgt ccttgccaca actttcccgg ttgcgcacgc
     9361 cgccgtaccg aaagatatgc tggtgattgg taaggccgcc gatccacaaa ccctcgaccc
     9421 ggcggtaaca atagataata acgactggac agtgacctac ccgtcttatc agcggctggt
     9481 tcagtacaaa acggacggtg ataaaggctc aaccgacgtt gaaggcgatc tggcaagtag
     9541 ctggaaagcg tctgacgatc aaaaagagtg gacgttcacc ctgaaggaca atgctaaatt
     9601 tgccgatggc acacctgtca ctgccgaagc agtaaaactc tcttttgagc ggttactaaa
     9661 aatcggccag gggccagcag aagcatttcc caaagattta aagattgata ctcccgacga
     9721 acatacggtg aagtttaccc ttagccaacc attcgcaccg ttcctctaca cgctagcgaa
     9781 tgacggtgca tccattatca atccggcggt cttaaaggaa catgcagcgg atgatgctcg
     9841 cggcttcctc gcgcaaaata ccgccggttc cggaccattt atgctgaaaa gcgggcaaaa
     9901 aggtcagcaa ttagttctgg tgccaaatcc gcattacccc ggcaataaac cgaactttaa
     9961 gcgagtatcg gtaaaaatta tcggtgaaag tgcctcccgt cgcctgcagc tctcccgtgg
    10021 cgacattgac attgccgatg cgctgccggt ggatcaactc aacgccctga agcaggaaaa
    10081 taaagtcaat gtggcagagt atccgtcact gcgcgttacc tatctgtatc tgaataacag
    10141 caaagcgcct cttaatcagg cggatctgcg tcgggccatt tcctggtcta ccgattatca
    10201 gggcatggtt aacggcattc tgagtggtaa cggaaaacag atgcgcggcc cgattccgga
    10261 aggcatgtgg ggctacgatg cgacggcaat gcaatacaac catgacgaaa cgaaagccaa
    10321 agccgaatgg gataaagtga cgagcaaacc caccagcctg acgtttctct actccgataa
    10381 cgatccgaac tgggagccta ttgctctggc gacacaatcc agtctcaaca agctgggcat
    10441 caatgtgaag ctggaaaagc tggcgaacgc caccatgcgc gacagagtgg gtaaaggtga
    10501 ttacgacatt gcgattggca actggagtcc ggattttgcc gacccgtata tgtttatgaa
    10561 ttactggttt gagtcagaca aaaaaggtct gccgggtaac cgctcgttct atgaaaacag
    10621 tgaggtcgat aagttactgc gcaatgcgct tgcgaccacc gaccagacgc agcgtacccg
    10681 ggactaccag caggcacaga aaatcgtcat tgatgacgct gcttatgtgt acctgttcca
    10741 gaaaaactac caactggcga tgaacaaaga ggtgaaaggc tttgtgttca atcccatgct
    10801 ggaacaggtc ttcaatatca ataccatgag taaataacgt gaccttctgg agtattttac
    10861 gccaacgctg ctgggggctg gtgctcgtgg tggcgggcgt ctgcgtgatt acgtttatta
    10921 tctcgcatct gatccctggc gatccggcgc ggttactggc gggtgaccgc gccagcgatg
    10981 ctatcgtgga aaatattcgc cagcaactgg gactggacca gccactgtat gtacagtttt
    11041 accgctactt cagcgacctg tttcatggtg acctgggaac atccattcgt accgggcgtc
    11101 cggtgctgga agagttgcgt atatttttcc cggcgacgct ggaactggct ttttgtgccc
    11161 tgctgctggc actcctgatt ggcatcccgc tgggcatact ctctgcagtc tggcgaaatc
    11221 gctggctgga tcatctggtg cgaataatgg ccattaccgg aatctccaca cctgcgttct
    11281 ggcttggact gggcgtcatt gtgctgtttt atggtcatct gcaaattctt cccggcggcg
    11341 gaaggcttga tgactggctg gatccaccaa cgcacgttac cggcttttat ctgctcgatg
    11401 cgctgcttga aggcaacggt gaagtcttct tcaatgcgtt gcaacatctc atcttaccgg
    11461 cattaacgct ggcgttcgtt cacctgggaa ttgtcgctcg ccagatccgc tcagcgatgc
    11521 tggaacaatt gagtgaagac tacattcgta ccgcccgggc cagcggcttg cccggctggt
    11581 atatcgtttt atgttatgcg ctacccaatg cgttgatccc atcgattacc gtattgggtc
    11641 tggcgctggg cgatttgttg tatggcgcag tgctcaccga aaccgttttt gcctggcccg
    11701 gaatgggtgc atgggtagta acatcaatac aggcgctcga cttcccggca gtgatgggct
    11761 ttgccgtcgt ggtttcattt gcttatgtgc tggtcaacct ggtggtggat ttgctctatt
    11821 tgtggattga tccgcgtatc ggacgtggag gtggtgaatg atgctaagcg aggaaacgtc
    11881 cgccgtacgc ccacaaaaac aaacgcgatt taacggtgca aaactggtat ggatgctgaa
    11941 aggcagtccg ctcaccgtga ccggcgcagt catcattgta ttaatgctat tgatgatgat
    12001 tttttcaccg tggcttgcga cgcatgatcc caacgccatt gatttaaccg cccgcctttt
    12061 gccgccttct gcggcgcact ggtttggcac cgatgaagtg ggacgcgatc tgttcagccg
    12121 cgtactggtc ggcagtcagc aatcaattct cgccggatta gtcgtggttg ccattgcggg
    12181 tatgattggt tcgctactcg gatgtctatc cggtgtgctt ggcggacgcg cagacgccat
    12241 catcatgcgc atcatggaca ttatgctgtc gattccttcg ttggtactga caatggcact
    12301 ggctgccgct ctcgggccga gtttatttaa cgccatgctg gcgattgcta ttgtgcgaat
    12361 tcccttttat gtgcgcctgg cgcgggggca agcattagtt gtacgccagt atacctatgt
    12421 tcaggcggcg aaaacctttg gtgcgtcgcg ttggcatctg atcagctggc atattttacg
    12481 taactccctg ccgccgctga tcgtgccggc atcgctggat atcggtagcg cgattttaat
    12541 ggccgccacg ttgggattta ttggcctggg tgctcaacaa ccgagtgctg aatggggcgc
    12601 gatggtagct aatggtcgca actatgtact cgatcagtgg tggtattgcg catttccggg
    12661 ggcagcgatt ttgcttaccg ccgtcgggtt taatctcttt ggtgatggta ttcgcgatct
    12721 gcttgacccg aaagcaggag gaaagcagtc atgacccaac ccgttctgga cattcaacag
    12781 ctgcatttga gtttccccgg ttttaacggt tatgttcacg tgctcaacaa tgtgtccttg
    12841 cagattaacc gcggtgaaat tgtcggtctg gtgggagaat ccggctcagg taaatcagtc
    12901 accgcaatgc tgattatgcg tctgctaccg acgggcagtt attgcgtaca tcgggggcag
    12961 atttcactgc tgggagacga tgttcttaac gctcgggaga aacagcttcg tcagtggcgc
    13021 ggcgcacgag tggcgatgat ctttcaggaa ccgatgaccg ccctcaatcc gacacgtcga
    13081 ataggtcttc agatgatgga cgtgatccgc catcatcaac caataagtcg tcgggaagcc
    13141 agagctaaag cgattaccct gctggaagag atgcaaatcc cggatgccgt ggaagttatg
    13201 tcgcgctatc cgtttgagct ttcaggtggt atgcgccagc gggtaatgat tgcgctggca
    13261 ttctcctgcg agccgcaatt gattattgcc gacgaaccga ctacggcgct ggacgtcacg
    13321 gtacagttgc aggtactgcg tttgcttaaa cataaagctc gcgccagtgg aactgcggta
    13381 ctgttcatca gccatgatat ggccgtggtg tcgcaactgt gcgatagcgt ttacgtgatg
    13441 tatgccggaa gcgtaattga aagcggcgtg acggcagacg ttatccatca tccccggcat
    13501 ccgtatacca ttggtttgct gcaatgcgca ccagaacatg gagtaccacg ccagccatta
    13561 cccgccattc cagggacggt accaaacctc acccatttgc ctgacggctg cgcttttcgc
    13621 gatcgttgct atgcggcagg tgcacagtgt gaaaacgtcc cggcgctgac agcgtgtggt
    13681 gacaacaacc agcgctgcgc ctgttggtat ccgcagcagg aggtcataag tatctgacac
    13741 gttattaacg ttacgcgacg tccatatcaa tttcccggcc cgtaaaagct ggcttggtaa
    13801 aactacggaa catgttcatg cgattaatgg tattgattta cagatccgcc gtggtgaaac
    13861 cttagggatc gtcggtgagt caggctgcgg caaaagcacc ctcgcacagc ttttaatggg
    13921 tatgctgcaa ccgagccacg ggcaatacat ccgttcagga tcacaacgca ttatgcagat
    13981 ggtgtttcag gacccgctct cttcgcttaa tccgcgctta tcggtgtggc gcatcatcac
    14041 agaaccgctc tggatagcta agcgtagtag tgaacaacag cggcgagcgt tggcaaagga
    14101 gttggctgtg caggtgggta ttcgtccgga gtatctcgac cgcctgcctc atgcgttctc
    14161 cggcgggcag cggcaacgca tcgccattgc cagagcactc tcttcgcagc ctgacgtgat
    14221 tgtgcttgat gagccaacct cggcgctgga tatctccgtg caggcgcaga tcctcaattt
    14281 actggtaacg ctacaggaaa atcacgggct gacttatgtg ctgatttcac acaatgtctc
    14341 ggtgatacgt catatgagcg atcgggtggc ggtgatgtat ctcgggcaga ttgtagaact
    14401 gggcgacgcg cagcaggtgc tgacggcacc tgcacatcca tacacccgat tattgctgga
    14461 ttccctcccc gccattgata aaccgctgga ggaagaatgg gcattatgta aaacggatct
    14521 gccgggaaac cgcacgttgc cacaaggctg ttttttctac gaacgttgcc ctctggcaac
    14581 ccacggatgt gaagtccggc aatcattagc aataagggag gacggacgtg agctccggtg
    14641 ctggcgggcg ctgtagagat gctaacaacg catcaggcat tcacgcgcct gacgcgtcat
    14701 ccggcaatgc tttacgattt caactggtaa tccagcgtaa tttccgcttt cagtacctga
    14761 gaaaccgggc atcctgcttt tgctttctgg atgatgccgt caaaggtaga ggcatcaata
    14821 cccggcaccg caacttcact cttcagtgcg attttcgtaa tcgcaaaatc ggcatccact
    14881 ttatccagcg acacatcggc ggtggtatca atcgatgttg gcgtgaatcc cgcttccccc
    14941 agcattaatg aaagcgccat tgagaaacat gcggcatgcg ctgcgccaat cagttcttca
    15001 gggttggttc ctttttcgcc ttcaaaacgc gtgttaaatc catacggctg ttggttcagc
    15061 acgccactct gggtggatac tgttcccttc ccgcgtttga tatcgccttc ccagtgtgcc
    15121 tgacctttct tatggattgt cattgttgct ctcctgtggg cttaaaaagg aaattataga
    15181 ctacgtgaga aacagcacga ggataagcag gatattccga ataaaatacg gcgaaaatat
    15241 tacttctgca cttaaattaa attcccacaa tagataaccg ggaatatata tctcaagaga
    15301 tattgcatca atatcaatca actcaccaca ataataatac atatcaatat catggagtta
    15361 aaatataaaa atcatcaacc aggactaatc ttaacaacga agcggcaaat atttgccatg
    15421 ctgaatgtgc tttataaatc tgcgatccgt agcagacacc ataaatacac agacacggag
    15481 aatcactatg tttacttatt atcaggcaga aaat
//
LOCUS       DAEEKQ010000090        15406 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.090, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000090 DAEEKQ010000000
VERSION     DAEEKQ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.090"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            10..453
                     /locus_tag="KLK57_002654"
     CDS             10..453
                     /locus_tag="KLK57_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0892984.1"
                     /translation="MDLALVTGQREGDLAAMRWEDIRDGRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGGDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            669..758
                     /locus_tag="KLK57_002655"
                     /pseudo
     CDS             669..758
                     /locus_tag="KLK57_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphatase"
     gene            complement(759..1817)
                     /gene="modC"
                     /locus_tag="KLK57_002656"
     CDS             complement(759..1817)
                     /gene="modC"
                     /locus_tag="KLK57_002656"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HBE0892985.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(1820..2509)
                     /gene="modB"
                     /locus_tag="KLK57_002657"
     CDS             complement(1820..2509)
                     /gene="modB"
                     /locus_tag="KLK57_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HBE0892986.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(2509..3282)
                     /gene="modA"
                     /locus_tag="KLK57_002658"
     CDS             complement(2509..3282)
                     /gene="modA"
                     /locus_tag="KLK57_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0892987.1"
                     /translation="MARKWLNLFAGAALSFAVAGNTLADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTASRQ
                     TLLGNSLVVVAPKASEQKDFIIDSTTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVVSKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="KLK57_002659"
     CDS             complement(3448..3597)
                     /gene="acrZ"
                     /locus_tag="KLK57_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HBE0892988.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            3726..4514
                     /gene="modE"
                     /locus_tag="KLK57_002660"
     CDS             3726..4514
                     /gene="modE"
                     /locus_tag="KLK57_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HBE0892989.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDIQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            4582..6054
                     /gene="modF"
                     /locus_tag="KLK57_002661"
     CDS             4582..6054
                     /gene="modF"
                     /locus_tag="KLK57_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HBE0892990.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     ESFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            6315..7331
                     /gene="galE"
                     /locus_tag="KLK57_002662"
     CDS             6315..7331
                     /gene="galE"
                     /locus_tag="KLK57_002662"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HBE0892991.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            7341..8387
                     /gene="galT"
                     /locus_tag="KLK57_002663"
     CDS             7341..8387
                     /gene="galT"
                     /locus_tag="KLK57_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HBE0892992.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            8391..9539
                     /gene="galK"
                     /locus_tag="KLK57_002664"
     CDS             8391..9539
                     /gene="galK"
                     /locus_tag="KLK57_002664"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HBE0892993.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            9533..10573
                     /gene="galM"
                     /locus_tag="KLK57_002665"
     CDS             9533..10573
                     /gene="galM"
                     /locus_tag="KLK57_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HBE0892994.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKSCPVNMTNHVYFNLDGEQSDVRNHKLQILAGEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            10776..11528
                     /gene="gpmA"
                     /locus_tag="KLK57_002666"
     CDS             10776..11528
                     /gene="gpmA"
                     /locus_tag="KLK57_002666"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HBE0892995.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="KLK57_002667"
     CDS             complement(11687..12739)
                     /gene="aroG"
                     /locus_tag="KLK57_002667"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HBE0892996.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPVTENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            13055..13435
                     /locus_tag="KLK57_002668"
     CDS             13055..13435
                     /locus_tag="KLK57_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HBE0892997.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEKHKNTMCKDGRCPD
                     INKKVQIGDGINNDVDTKTDGTTQ"
     gene            13549..14490
                     /gene="zitB"
                     /locus_tag="KLK57_002669"
     CDS             13549..14490
                     /gene="zitB"
                     /locus_tag="KLK57_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HBE0892998.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSIVVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVITLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="KLK57_002670"
     CDS             complement(14487..15206)
                     /gene="pnuC"
                     /locus_tag="KLK57_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HBE0892999.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(15244..>15406)
                     /gene="nadA"
                     /locus_tag="KLK57_002671"
     CDS             complement(15244..>15406)
                     /gene="nadA"
                     /locus_tag="KLK57_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HBE0893000.1"
                     /translation="CAHCPWMAMNGLQSIAEALEQEGSNHEVLDERLRERALVPLNRM
                     LDFAATLRG"
BASE COUNT         3917 a         4043 c         3850 g         3596 t
ORIGIN      
        1 ggcctttgca tggatcttgc actggtaaca ggacagcgcg aaggagatct ggcggctatg
       61 agatgggagg atataagaga tggcaggcta tacgtggaac aacaaaaaac aggggcaaaa
      121 attcgcattt ctctccccac cacgatttcc agattgaatt taacacttgc cgatgtactg
      181 gataacttga aaaaaataaa cggtaaaaac gaaaagttac tcggagggaa gacagccagg
      241 acaatcgcag cacagttcag gattgcccga gacacatccg gattgaaatg ggaaggtgac
      301 ccaccgccat ttcatgaaat ccgaagttta tccggtcgcc tgcactcagc agaaaaaggc
      361 ggtgatttca ctcaggcgct ccttggacat cgttcatcca gcatgacgga taaataccgt
      421 gacggacgag ggagggaatg gaaagacatt taaaattaat ttcgaatgat ttcgaaacac
      481 cacttgtaat taattgatta taaatgatta attcctaatg ggcatttgct tcatgaatgc
      541 tcctttgcat ttagcaactg actgtaacag ctaaaattag tcgcttttgg cggtgaatgc
      601 tgaattaaaa cgcagaattt tgtccgatag tggtatcggt gtaccattcg cgcagacgaa
      661 aattcttaaa tgacgcggta aaagcgcgcg ccaacattgt gattggtgat aacaccaccg
      721 acagcattgc ccagttcatt tatagccacc tgatttaatc aggcggttat cgacacactt
      781 ttaatttgcg cgtacagcca caggccaggt ttgatcgcca gttcatccct ggcccacggg
      841 ctgatacgcg cccacagcgt tttaccgccg acttccagct ccacttccac ctggccgttg
      901 tcgtcataac tattaacaac ttttgcccgc aatacgttac gaatgctggt ttgctgcggc
      961 ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga tacgtagcgc agcttgcagc
     1021 ggttcgtcca gcttattgac ccacaaatgc tgatcgccca gcgccagcgc ggtcatcgcg
     1081 taatgcggat gatgctcaag taccgtcact ttcagaatgc tactttgttg ctctttcggc
     1141 agccacggat tcatcacgct actgccccac acttcctcca gcgcgccaaa ggctttcacc
     1201 tgaccgtttt ccagtaccat cactctgtct gccagatgga ggatctcatc cagcgaatgg
     1261 ctgacataca acatcggaat gttgatttcc cgtgtcagcc gttgcagata aggcaacagt
     1321 tcgcgtttac gcggaatatc cagtgacgcc agcggttcat ccagcaacag caattccggt
     1381 gctgtcagca aagcccgacc aatcgccacg cgctgttttt cgcctccgga caggctgcct
     1441 ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca ccagcttatc gaactgatcg
     1501 accatacttt tcgacatgcc gtagcgcaga ttgccacgca ctttgtaatg cgggaacagc
     1561 cgcgcatcct gaaaaacata gccaacgcga cgcttttccg gcgtcaggca gatacctttt
     1621 tcggcatcat ttagtacccg cccattgagg acaatccgcc ctttttgcgg gcgcgtcagt
     1681 ccactgatgg cgttaatcag cgaagttttt ccggcaccgg agacgccaaa gatagcagtg
     1741 atgccattgg cgggcagtgt ttcattaatg gtcaggcaat ggttgcccaa cgtctgggaa
     1801 aaattcagct ccagcatgat tagcgccccg cccgttcacg gctgattctg gccagccatt
     1861 ctgaaatcaa cagggagatc atcgccagcg caatagaaat aatgcacagt ctcgccgcgc
     1921 cactttcccc gccgggggtc tggatcaggg tatacatggc agagggaatg gttcgcgttt
     1981 cgccaggaat gttcgacaca aaggtgatgg ttgcaccaaa ctcaccgaga gaacgagcaa
     2041 aagccagtac cgtaccaaca ataattcccg gtaaggtcag cggtaacgtg atagtaaaga
     2101 aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg ttcgagtttg acatcaaccc
     2161 cttccagcgc cagacgaatt gcccgcacca tcagcggaaa cgacatgacg gcggcagcga
     2221 gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa ccagtcatac agacgttcac
     2281 cgataaatcc gcgccgtccc atcgaaacta ataataagta accgacgacc acgggcggta
     2341 acaccagcgg tagatgcagt acgctgtcga gcagagcttt gcccggaaac gtgcaacgca
     2401 ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa cagcacagcc agggaagaaa
     2461 ctttcaggct taataaaact gcctgccatt ctggatcggt cagtatcatt acttggttgt
     2521 aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc ttcagataat cataaaatgc
     2581 tttcactgtc gcattgttat gcccttccac aacagcaacc ggatattcca cttttttatg
     2641 tgaatcttcc gggaaggtgg caaccacttt tacccctttg ctgacaactg cgtcagaacc
     2701 gtaaacaatg cccaacggcg cttcattgcg ttcgaccaac gccagcgcgc cacgaacatc
     2761 ttccgccgga gccagtttcg gggagagcgt atcccatgcg cccagttttt gcagtgcttc
     2821 ttttgcataa atgccagcag ggacatgttc cggatcgcca accgcgaggc gaccgccatt
     2881 cagcagcgaa gtccagttag ttgtgctgtc gatgatgaaa tctttctgct cgctggcttt
     2941 cggtgctaca acgaccaggc tattgccgag cagtgtctgg cgcgaagccg tatcgatcgc
     3001 ttttttatca accgcataat ccatccattt ctgatcggca gagataaaca gatccgcagg
     3061 cgctcccgct tcaatctgac gggccagagt agatgacgaa gcgaaagaag aaaccacatc
     3121 cacgcctttt tcttttttat actgcgtagc aatgtcctgc attgcgttag tcagtgatgc
     3181 ggcggcgaac accgtgattt tcccttcatc tgccagtgta ttgccagcaa cagcgaaaga
     3241 gagtgccgcc ccggcaaaca agttcaacca tttacgagcc attggtaacc ccttaatgta
     3301 acgttatata ggtgacaatg taacggctag ctcagaattt tcccagcagt tattgctaac
     3361 ctttgtgagg tagataagaa aaaatatcgg ctggaaaagc aggaagttga gaaaaagaaa
     3421 tgcccgacta agcgggcatt cagggaatca atgattttgt ccgggctggt cttttttacc
     3481 aacaccagaa aagatgttga atacttcacc aagaccgtaa atcagaccca ggatgatggc
     3541 catcacgaca ggtaccatga ttacggcgaa taccagactt tttaataact ctaacatggt
     3601 caactccaga tatagtcatg agactattct aaccgctaag cacagaaaag cactcccctt
     3661 ttgtgcggtc agctttgcgt ggcgttcgtt ttccgtcaca ataagacttt tgccaggaca
     3721 ttgttatgca ggccgaaatc cttctcaccc ttaagctcca acaaaaatta ttcgccgacc
     3781 cgcgccggat ttcgctacta aaacacattg cgctttccgg ttccattagc cagggagcga
     3841 aagatgccgg tatcagctat aaaagcgcct gggatgccat taacgagatg aatcagttaa
     3901 gcgagcatat tctggttgag cgcgcaacag gcggtaaagg tggcggcggc gcagtactga
     3961 cccgctatgg tcagcgactg attcagctct atgacttact ggcgcaaatc cagcaaaaag
     4021 cctttgatgt gttaagtgac gatgacgccc tgccgctgaa cagcctgctg gccgcgatct
     4081 cacgtttttc actgcaaacc agcgcccgta accagtggtt cggtaccatc accgcccgcg
     4141 atcatgatga cattcaacag catgttgatg tcttactggc tgacggaaaa acacgcctga
     4201 aagtcgcaat taccgcacaa agcggcgcac gtctggggct ggatgaaggc aaagaagtgt
     4261 tgatattgct aaaagcgccg tgggtaggca ttactcagga cgaagcggtc gcacaaaacg
     4321 ccgacaacca attaccgggt attattagtc atattgagcg cggcgcagag cagtgcgaag
     4381 tattaatggc gctacccgac gggcaaacac tgtgcgccac agtgccggta aatgaagcga
     4441 cttctcttca gcaagggcag aatgtcacgg cctactttaa tgccgacagc gtgattatcg
     4501 ccacgctgtg ctaagcgtgt tgacaatttg ttatgaaaca cgtatccctg tcagtaatcg
     4561 ctgcacaaaa tgggatataa aatgtcatcg ttgcaaattt tgcaaggcac gtttcgtctt
     4621 agcgacacaa aaacgctgca attgcctcag ctaacgttaa acgcgggtga tagttgggcg
     4681 tttgtcggtt cgaatggaag cgggaaatcg gccctggccc gcgcgctggc gggggaactt
     4741 ccgcttttga aaggtgaacg gcaaagccag ttttcccaca tcactcgtct ctccttcgag
     4801 caattgcaaa aactcgtcag cgacgaatgg cagcggaata acaccgatat gctcggccct
     4861 ggcgaagatg acaccggacg cactacggct gaaatcattc aggatgaagt aaaggatgca
     4921 ccgcgttgca tgcaactggc gcagcagttc ggtattaccg ccctcctcga ccgacgcttt
     4981 aaataccttt ccactggcga gacgcgaaaa accctgctgt gtcaggcgct gatgtcggag
     5041 cctgacttgt tgattcttga tgagtcgttc gatggcctgg atgttgcctc acgtcagcag
     5101 ctggctgagc gactcgcctc gttacatcag tccggtatta ctctggtact ggtgctcaat
     5161 cgcttcgatg agatcccgga gtttgtccag tttgctggcg tgctggcgga ttgcacgtta
     5221 gcggaaactg gcgctaaaga ggaactgctc caacaagcac tcgtcgcgca actggcgcat
     5281 agtgaacagc ttgaaggtgt gcaactgccg gagccggatg aaccttcagc acgtcacgcc
     5341 ttacccgcca acgaaccgcg cattgtgctg aacaatggcg tggtttctta taacgatcgc
     5401 cccattctta ataaccttag ctggcaggtg aatccaggtg aacactggca aattgtcggg
     5461 ccaaatggtg caggaaaatc gacgttatta agcctggtta ctggcgatca tccgcaaggt
     5521 tacagcaacg atttgacgct tttcggacga cgtcgcggca gcggcgaaac catctgggat
     5581 atcaaaaagc atatcggtta cgtcagcagt agtttgcatc tggattaccg ggtcagcact
     5641 accgtgcgta atgtgattct ttctggctat tttgattcga ttggcattta tcaggccgtt
     5701 tcggatcgcc agcaaaaact ggtgcagcag tggctggata ttctcggcat tgataaacgt
     5761 acggctgacg ctccgttcca tagtctttcc tggggacagc agcgtctggc gctgatcgtc
     5821 cgcgcactgg tgaaacatcc gacgttgctt attctcgatg aaccactaca ggggcttgat
     5881 ccgctcaatc gccagcttat ccgccgtttt gttgatgtgc tgattagcga aggtgaaacg
     5941 caattgttat ttgtttcgca ccacgctgaa gatgcgcctg cctgtattac ccatcgcctt
     6001 gagttcgtgc cggacggtgg actctatcgc tatgtgctga caaaaatata ttgagtcggt
     6061 agtgctgacc ttgccggagg cggcctaagc gccctctccg gccaacggtt cgacgcatgc
     6121 aggcatgaaa ccgcttcttt tttcagataa aaagcgcaat cattcataaa ccctctgttt
     6181 tataatcact taatcgcgca taaaaaacgg ctaaattctt gtgtaaacga ttccactaat
     6241 ttattccatg tcacactttt cgcatctttg ttatgctatg gttatttcat accataagcc
     6301 taatggagcg aattatgaga gttctggtta ccggtggtag cggttacatt ggaagtcata
     6361 cctgcgtgca attactgcaa aacggtcatg atgtcatcat tcttgataac ctctgtaaca
     6421 gtaagcgcag cgtactgcct gttatcgagc gcttaggcgg caaacatcca acgtttgttg
     6481 aaggcgatat tcgtaacgaa gcgttgatga ccgagatact gcacgatcac gctatcgaca
     6541 ccgtgatcca cttcgccggg ctgaaagccg tcggcgaatc ggtacaaaaa ccgctggaat
     6601 attacgacaa caatgtcaac ggcactctgc gcctgattag cgccatgcgc gccgctaacg
     6661 tcaaaaactt tatttttagc tcctccgcca ccgtttatgg cgatcagccc aaaattccat
     6721 acgttgaaag cttcccgacc ggcacaccgc aaagccctta tggcaaaagc aaattgatgg
     6781 tggaacagat cctcaccgac ctgcaaaaag cccagccgga ctggagcatt gccctgctgc
     6841 gctacttcaa cccggttggc gcgcatccgt cgggcgatat gggcgaagat ccgcaaggca
     6901 ttccgaataa cctgatgcca tacatcgccc aggttgctgt aggccgtcgc gactcgctgg
     6961 cgatttttgg taacgattat ccgaccgaag atggtactgg cgtacgcgat tacatccacg
     7021 taatggatct ggcggacggt cacgtcgtgg cgatggaaaa actggcgaac aagccaggcg
     7081 tacacatcta caacctcggt gctggcgtag gcagcagcgt gctggacgtg gttaatgcct
     7141 tcagcaaagc ctgcggcaaa ccggttaact atcattttgc accgcgtcgc gaaggcgacc
     7201 ttccggccta ctgggcggac gccagcaaag ccgaccgtga actgaactgg cgcgtaacgc
     7261 gcacactcga tgaaatggcg caggacacct ggcactggca gtcacgccat ccacagggat
     7321 atcccgatta aggaacgacc atgacgcaat ttaatcccgt tgatcatcca catcgccgct
     7381 acaacccgct caccgggcaa tggattctgg tttcaccgca ccgcgctaag cgcccctggc
     7441 agggggcgca ggaaacgcca gccaaacagg tgttacctgc gcacgatcca gattgcttcc
     7501 tctgcgcagg taatgtgcgg gtgacaggcg ataaaaaccc cgattacacc gggacttacg
     7561 ttttcactaa tgactttgcg gccttgatgt ctgacacgcc agatgcgcca gaaagcaacg
     7621 atcctttgat gcgttgccag agcgcgcgcg gtaccagccg ggtgatctgc ttttcaccgg
     7681 atcacagtaa aacgttgcca gaactgagcg ttgcggcatt gacggaaatc gtcaaaacct
     7741 ggcaggagca aaccgcagag ctgggaaaaa cgtacccgtg ggtgcaggtc tttgaaaaca
     7801 aaggtgcggc gatgggctgc tctaacccgc atccgcacgg acagatttgg gcaaatagct
     7861 tcctgcctaa cgaagctgag cgcgaagacc gcctgcaaaa agaatatttt gccgaacaga
     7921 aatcaccaat gctggtggat tatgttcagc gcgagctggc agacggtagc cgtaccgttg
     7981 tcgaaaccga acactggtta gccgttgtgc cttactgggc tgcctggccg ttcgaaacgc
     8041 tactgctgcc caaagcccac gttttgcgga tcaccgattt gaccgacgcc cagcgcagcg
     8101 atttggcgct ggcgttgaaa aagctgacca gtcgttatga caacctcttc cagtgctcct
     8161 tcccctactc tatgggctgg cacggcgcac catttaatgg cgaagagaat caacactggc
     8221 agctgcacgc gcacttttat ccgcctctgc tgcgctccgc caccgtacgt aaatttatgg
     8281 ttggttatga aatgctggca gagacccagc gagacctgac cgcagaacag gcagcagagc
     8341 gtttgcgcgc agtcagcgat atccattttc gcgaatccgg agtgtaagaa atgagtctga
     8401 aagaaaaaac acaatctctg tttgccaacg catttggcta ccctgccact cacaccattc
     8461 aggcgcctgg ccgcgtgaat ttgattggtg aacacaccga ctacaacgac ggtttcgttc
     8521 tgccctgcgc gattgattat caaaccgtga tcagctgtgc accacgcgat gaccgtaaag
     8581 ttcgcgtgat ggcagccgat tatgaaaatc agctcgacga gttttccctc aatgcgccca
     8641 ttgtcgcgca tgaaaactat caatgggcga actacgttcg tggcgtggtg aaacatctgc
     8701 aactgcgtaa caacagcttc ggcggtgtgg acatggtgat cagcggcaat gtgccgcagg
     8761 gtgccgggtt aagttcttcc gcttcactgg aagtcgcggt cggaaccgta ttgcagcagc
     8821 tttatcatct gccgctggac ggcgcacaaa tcgcgcttaa cggtcaggaa gcagaaaacc
     8881 agtttgttgg ctgtaactgc gggatcatgg atcagctaat ttccgcactc ggcaagaaag
     8941 atcatgcctt gctgattgac tgccgctcac tggggactaa agcagtttcc atgccgaaag
     9001 gtgtggctgt cgtcatcatc aacagtaact tcaaacgtac cctggttggc agcgaataca
     9061 acacccgtcg tgaacagtgc gaaaccggtg cgcgtttctt ccagcagcca gctctgcgcg
     9121 atgtcaccat tgaagagttc aacgctgttg cacatgagct ggacccaatc gtggcgaaac
     9181 gcgtgcgtca tatcctgact gaaaacgccc gcaccgttga agctgccagc gcgctggagc
     9241 aaggcgatct gaaacgtatg ggcgagttga tggcggagtc tcatgcctct atgcgcgatg
     9301 atttcgaaat caccgtgccg caaattgaca ctctggtaga aatcgtcaaa gcagtgattg
     9361 gcgacaaagg tggcgtacgc atgaccggcg gcggatttgg cggctgtatc gtcgcgctga
     9421 tcccggaaga gctggtgcct gccgtacagc aagctgtcgc tgaacaatat gaagcaaaaa
     9481 caggtattaa agagactttt tacgtttgta aaccatcaca aggagcagga cagtgctgaa
     9541 cgaaactccc gcactggcac ccgatggtca gccgtaccga ctgttaactt tgcgtaacaa
     9601 cgcagggatg gtagtcacgc tgatggactg gggtgcgact ttactttcag ctcgtattcc
     9661 gctttccgat ggcagcgtcc gcgaggcgct gctcggctgt gccagcccgg aatgctatca
     9721 ggatcaggcc gcgtttctgg gggcctctat tggtcgttat gccaaccgca tcgccaatag
     9781 ccgttatacc tttgacggtg aaacggtgac gctttcgcca agtcagggcg ttaaccagct
     9841 gcacggcggc ccggaagggt tcgacaaacg tcgctggcag attgtgaacc agaacgatcg
     9901 tcaggtgctg tttgccctga gttcagatga tggcgatcag ggcttcccgg gtaatctcgg
     9961 cgcgacggtg caatatcgtc tgaccgacga taaccgtatc tccattactt atcgcgccac
    10021 agttgataaa tcttgcccgg tgaatatgac taatcacgtc tatttcaatc ttgacggcga
    10081 gcagtctgac gtgcgcaatc acaagttgca gattctggcg ggcgaatatc tgccggttga
    10141 tgaaggcggc attccgcacg acggcctgaa atctgtcgcc ggaacctctt ttgatttccg
    10201 cagcgccaaa atcatcgcca gtgagtttct tgccgacgac gatcagcgca aagtgaaagg
    10261 ttacgatcac gcattcttgt tacaggccaa aggcgatggc aagaaagtgg cggcgcatgt
    10321 ctggtcagca gatgaaaaat tgcagctgaa ggtctacacc accgctcccg ctcttcaatt
    10381 ctactccggc aacttcctcg gcggcacacc gtcgcgggga accgaacctt acgccgactg
    10441 gcaaggactg gcgttggaaa gcgagtttct gccggacagc ccgaaccacc ctgaatggcc
    10501 gcaaccggac tgcttcctgc gtcctggcga agagtattcc agcctgacgg aatatcagtt
    10561 tattgctgag taatgttaag ccctcgtcat gagggcttta tcttcatatt gttcaaatca
    10621 ccagcaaaca ccgacatatt tgcaactcaa tattcacaac aaccttacac tgcgccacta
    10681 ttttcgctat ggttatgcgt aagcattgct gttgcttcgt cacggcaata taatgagaat
    10741 tattatcata aaaagataat ttgaggagta agtatatggc tgtaactaag ctggttctgg
    10801 ttcgtcatgg cgaaagtcag tggaacaaag aaaaccgttt caccggttgg tacgacgtgg
    10861 atctgtctga gaaaggcgta agcgaagcaa aagcagcagg taagctgctg aaagaggaag
    10921 gttacagctt tgactttgct tacacttctg tgctgaaacg cgctatccat accctgtgga
    10981 atgtgctgga cgaactggat caggcatggc tgcccgttga gaaatcctgg aaactgaacg
    11041 aacgtcacta cggtgcgttg cagggtctga acaaagcgga aaccgccgaa aagtatggcg
    11101 acgagcaggt gaaacaatgg cgtcgtggtt ttgccgtgac tccgccggaa ctgactaaag
    11161 atgatgagcg ttatccgggt cacgatccgc gttacgcgaa actgagcgag aaagagctgc
    11221 cgctgactga aagcctggcg ctgaccattg accgcgtgat cccttactgg aatgaaacta
    11281 ttctaccgcg tatgaagagc ggtgagcgcg tgatcatcgc tgcacacggt aactctttac
    11341 gtgcgctggt gaaatatctt gataacatga gtgaagaaga gattcttgag ctgaatatcc
    11401 cgaccggcgt gccgctggtg tatgagttcg acgagaattt caaaccgctg aaacgctatt
    11461 atctgggtaa tgctgacgag atcgcagcga aagcagcggc ggttgctaac cagggtaaag
    11521 cgaagtaaac gtcattcgtt taaaaatgag aaagccgact gcaagtgagt cggctttttt
    11581 gttgctaaca atggagcaca ttgcctgatg cgacgctgcg cgtcttatca ggcctgtggt
    11641 gattcatcgg atacgccact ctgacggcgc atccgacaat taaaccttac ccgcgacgcg
    11701 cttttactgc attcgccagt tgacgtaaca gagcatcggt atcttcccag ccaatgcagg
    11761 catcggtgat gctcttaccg taggccagcg gctccccgct atcgaggctc tgattgcctt
    11821 ccaccagatg gctttccacc atcacgccaa taatggcctt ttcgccaccg gcaatctgct
    11881 ggcaaacgtc agcacaaaca tccatctgct ttttgaattg tttggacgag ttagcatggc
    11941 tgaaatcgat catcacctgt gctggcaggc ctgctttgtt cagcccttct ttcacttcag
    12001 caacgtgctt cgcgctgtag ttaggctctt taccgccgcg cagaatgata tggcaatcac
    12061 cgttaccgct ggtattcaca atcgccgaat gcccccattt agttacggac aggaagcagt
    12121 gcggcgcacc ggcggcatta atggcatcga tagccacttt aatcgtaccg tctgtgccat
    12181 ttttaaaacc taccggacaa gaaagaccag acgccagttc gcggtgcacc tgcgattccg
    12241 tggtacgtgc accaattgcg ccccagctca tcaggtcagc gagatactga ggagtgatca
    12301 tatcgagaaa ctcacccgcc gctggcagac cgctgtcgtt aatatcaagc agcaatttac
    12361 gggctatacg cagaccgtcg ttgatctgga agctgttatc catatgcgga tcgttaatca
    12421 accctttcca gcccaccgtg gtacgcggct tttcaaaata gacgcgcatt acgatttcca
    12481 gctcatcttt cagctcttca cgcagcgcca gcaagcgagt ggcatactct ttagccgcga
    12541 caggatcatg aattgagcac gggccaatca ccaccaacag gcgatcatca ttacctttca
    12601 ggatcttatg gatcgctttt cgggcatggg caaccgtatt cgcggcattt tcagttacgg
    12661 ggaatttttc cagcaatgcg acaggaggaa gtaactcttt gatttctttg atgcgtaaat
    12721 cgtcgttctg ataattcatg tctgttccag tgttgccata cttatcttag tgaatgcaat
    12781 acttccaatc tatatcttcc gtcagaatgt gtaaacgggg ttttacacta tgaacggatt
    12841 aatcctggaa ttagcaagaa aaacgccaga ttgtcgcgaa aaacgagatc tctcctacaa
    12901 tttctaactg taactccttt gtttgttaat tatttcaaga ttctctgctg cgtttcataa
    12961 actggctgaa atcttaaacc aatgccttat attcacctgc aaatgcactg ttggaagagg
    13021 ttatccgaca taacgaccat aacaggagca tcctatgaaa atgacaaaac tggccacatt
    13081 atttctgact gccactctaa gccttgccag cggtgccgca ctggctgccg atagcggagc
    13141 gcaaactaat aacggccagg caaacgccgc agctgatgcg ggccaggtag cccctgacgc
    13201 ccgtgaaaat gtcgcgccaa ataacgtcga caataacggg gtaaataccg gttctggcgg
    13261 cacaatgctg cattcggatg gttcttcaat gaacaatgac ggaatgacca aagatgaaaa
    13321 gcacaagaac accatgtgca aagatggtcg atgtccggat atcaataaaa aggtacaaat
    13381 cggtgatggc atcaataatg atgtcgatac caaaaccgac ggtaccacgc agtaaccctg
    13441 caaaatagcc aataatggga gagcggagtc tctccctttt aatttttcac ttctaaaagc
    13501 agtatgatgt cataaagtta agtgagaata attaagggat gactcgtcat ggcgcactca
    13561 cactcacaca cgtcctcaca cctgccagaa gataataatg ctcgtcgctt gttgtatgct
    13621 ttcggcgtga ctgccgggtt tatgctggta gaagtcgttg gtggttttct ttctggttct
    13681 ctggcattgc tggccgatgc gggtcatatg ttgaccgata ctgccgccct gcttttcgcc
    13741 ctactcgccg tgcaattttc acgtcgtcct cccaccattc gccacacttt cggttggcta
    13801 agactgacca cgctcgcggc gtttgtgaat gccatcgccc tcgtggtaat taccattttg
    13861 attgtctggg aggcgataga acgcttccgc acgccgcgtc cggtcgaggg cggcatgatg
    13921 atggcaattg ccgtggctgg gttgctggca aatatacttt ctttctggtt acttcatcac
    13981 ggcagcgaag agaaaaacct caacgtacga gcagcggcat tgcatgtgct cggggacctg
    14041 ttgggttcgg ttggggctat tattgccgcg ttgattatta tctggaccgg ctggacacct
    14101 gctgacccca ttctctcgat agtggtgtcg cttctggttc tgcgtagcgc gtggcgactg
    14161 ttgaaagata gcgtgaatga attacttgaa ggtgcaccgg tatcgctgga tatcgctgaa
    14221 ctgaagcgtc gtatgtgccg ggaaatcccg gaagtccgca atgtgcacca tgtacatgta
    14281 tggatggtag gcgagaagcc ggtgataacg ctgcatgtgc aggtgatccc gccacacgat
    14341 cacgatgcct tgttggatca gatccaacac tacctgatgg atcactatca gattgagcac
    14401 gccaccattc agatggaata tcaaccttgt catgggccag actgccacct gaatgaaggg
    14461 gtgtctggcc attcacatca tcaccattaa tgggacagcg cgcgtgagcc tctttcacgt
    14521 gcgctgttga tccacatccg actgccgttg agcgcaataa aggtcaggat gatgtactcc
    14581 agagacatgg cgtaaacgcc ctgaagtgca aaaataacga cgctaatcac gttaataatc
    14641 acccacaaca gccagttttc cacatactta cgcgtcatca gaatcattgc cacgatagat
    14701 aacaccatca tgcatgaatc ccagaacggg aaagcgtccg gttgcagttc aggcatcaca
    14761 acctgtaatc ctaatgcttg catgatcatg actgccacgc gggtcaaaaa tgcaaacacc
    14821 ggattgataa ataccgtcat cagaccaatc gaaacaacgc aaaccgccaa ccagctgagc
    14881 gccttcggca atggtaacca gcgaattttc aactccgcct cgttctgact ggtttgtcgc
    14941 gaccacgcat accaaccgta aatattcgcg gcaaagaaaa acacctgtaa tagcaggctg
    15001 gcatacagct gaatctgaaa gaaaataatg ccaaacaagg tgacgttgat caggccaaag
    15061 aagtagttgc tgatcttctc cagactggca aggccaatac acagcaaccc ggcgatcgtg
    15121 cctaccgctt cgatccatga gagatcataa ccgcctgccc ctattggtat atgtaccagg
    15181 atattctgca cactaaaaaa atccatcttt cccccaaagc gtaacgttac gccttattat
    15241 tcgttatcca cgtagtgtag ccgcaaaatc cagcatacga ttgagcggca ccagcgccct
    15301 ctctcgcagc ctttcatcaa gaacctcgtg attgcttcct tcctgttcta atgcctctgc
    15361 gatggactga aggccattca tggccatcca cggacaatgc gcgcag
//
LOCUS       DAEEKQ010000091        15348 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.091, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000091 DAEEKQ010000000
VERSION     DAEEKQ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15348
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.091"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(266..1297)
                     /gene="rsmC"
                     /locus_tag="KLK57_002672"
     CDS             complement(266..1297)
                     /gene="rsmC"
                     /locus_tag="KLK57_002672"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HBE0893001.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATVDDVADCDMLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            1400..1813
                     /gene="holD"
                     /locus_tag="KLK57_002673"
     CDS             1400..1813
                     /gene="holD"
                     /locus_tag="KLK57_002673"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HBE0893002.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            1782..2228
                     /gene="rimI"
                     /locus_tag="KLK57_002674"
     CDS             1782..2228
                     /gene="rimI"
                     /locus_tag="KLK57_002674"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HBE0893003.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            2243..2920
                     /gene="yjjG"
                     /locus_tag="KLK57_002675"
     CDS             2243..2920
                     /gene="yjjG"
                     /locus_tag="KLK57_002675"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HBE0893004.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            3011..4600
                     /gene="prfC"
                     /locus_tag="KLK57_002676"
     CDS             3011..4600
                     /gene="prfC"
                     /locus_tag="KLK57_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HBE0893005.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYSVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            4993..5598
                     /gene="osmY"
                     /locus_tag="KLK57_002677"
     CDS             4993..5598
                     /gene="osmY"
                     /locus_tag="KLK57_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HBE0893006.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            5725..5886
                     /locus_tag="KLK57_002678"
     CDS             5725..5886
                     /locus_tag="KLK57_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HBE0893007.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            6008..7081
                     /locus_tag="KLK57_002679"
     CDS             6008..7081
                     /locus_tag="KLK57_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HBE0893008.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            7078..7860
                     /locus_tag="KLK57_002680"
     CDS             7078..7860
                     /locus_tag="KLK57_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE0893009.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRIHDKLAMHLKRHDLPCTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAVRVFDVLCELRPEPEDEIAEVLLNNTYALFSVSG"
     gene            complement(8376..9239)
                     /locus_tag="KLK57_002681"
     CDS             complement(8376..9239)
                     /locus_tag="KLK57_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HBE0893010.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQHLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(9211..10761)
                     /locus_tag="KLK57_002682"
     CDS             complement(9211..10761)
                     /locus_tag="KLK57_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HBE0893011.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGSSSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            11019..11798
                     /gene="deoC"
                     /locus_tag="KLK57_002683"
     CDS             11019..11798
                     /gene="deoC"
                     /locus_tag="KLK57_002683"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HBE0893012.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            11925..13247
                     /gene="deoA"
                     /locus_tag="KLK57_002684"
     CDS             11925..13247
                     /gene="deoA"
                     /locus_tag="KLK57_002684"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HBE0893013.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            13299..14522
                     /gene="deoB"
                     /locus_tag="KLK57_002685"
     CDS             13299..14522
                     /gene="deoB"
                     /locus_tag="KLK57_002685"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HBE0893014.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAVGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            14602..15321
                     /gene="deoD"
                     /locus_tag="KLK57_002686"
     CDS             14602..15321
                     /gene="deoD"
                     /locus_tag="KLK57_002686"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HBE0893015.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
BASE COUNT         3622 a         4016 c         4173 g         3537 t
ORIGIN      
        1 accatcaatt cttaaaaaga attgctacca cggaggcgca ttctagtggt tttcagcttt
       61 tcgtcaatag ttaattatcg ccggaggtgt aattgctgga aaaatgtcca tcaggaaact
      121 agcgtgcagg tttggtaggc atgcgggggc agatgccaga tgcgacgctg gcgcgtctta
      181 tctggcctac gaagggctaa cgtgcaggtt ttgtaggtcg gataaggcgt tcacgccgca
      241 tccgacacgg tattcggcga gataattaac ctttcttcgc ctggcgggtc ataatggcgc
      301 gatacacctt gaaacgcccg gtttgcgcaa tcacttcatg gaagccaaat gtctcatcca
      361 gcacgtccgg gtaaggcagg aaggcgttcg ctacaattcg cagctcgccg ccgctattaa
      421 gatgacgcac cgcgccgcga atcagcgttt gcgccgcatc caggctggtt tgcatcccat
      481 cgtggaacgg cgggttggag atgatcatat caaaacgacc tttcacctcg gaaaagacgt
      541 tgctggcaaa gacttcacct tcaacacagt tggccgcaag tgttgcgcgg ctggcttcta
      601 ccgctggcgc agagacatcg cacaaggtga gacgaatttt cggcgaatgg cgcgcaaagg
      661 caactgaaag cacccccgcg ccacagccga catccagcac tttacctttc gtgtgcggag
      721 ttaacgtcga gagcagcaac tggctaccga catccagacc gtcgcggcta aacacgccag
      781 gcagcgtttt gaccgtcagg ccatcgacgc tgtattcgcc ccagaattta tcggcatcaa
      841 ataccggctg tttttccaga cgaccaaaat agaggccaca gcgacgagcg ctgtcgactt
      901 tattcaacgg cgcataatct gccagcatct gctcggcgct gcgcacgccg ctgcggttct
      961 cgccaacgac aaaaatatct gtccccactg gcagcagaga aagtaaattc atcaactgga
     1021 actgggcttc cggtttgttc ttcggccagt agtaaatcag catatcgcaa tctgcgacgt
     1081 catccaccgt ggcgaccaga ctgaaacggg cgttatcccc catctggcgg cttaatacct
     1141 gccagtggtg gaattgctgg gtatgagcac ggctggccgc ggtatctaaa cgcgcgggca
     1201 ggtcatcctg taagtctccg gcaaacagaa tacggctttg ttcgaaatca tcactgtgac
     1261 gcagcaagac ttcacttgcc ggggtaaatg cagacatgga atgctcctca attgatactg
     1321 gcggcgatta tagccatatg ttggcgcggt atcgacgaat ttgctatatt tgcgcccctg
     1381 acaacaggag cgattcgcta tgacatcccg acgagactgg cagttacagc aactgggcat
     1441 tacccagtgg tcgctgcgtc gccctggcgc gttgcagggg gagattgcca ttgcgatccc
     1501 ggcacacgtc cgtctggtga tggtggcaaa cgatcttccc gccctgaccg atcctttagt
     1561 gagcgatgtt ctgcgcgcat taaccgtcag ccccgatcaa gtgctgcaac tgacgccaga
     1621 aaaaatcgcg atgctgccgc aaggcagtcg ctgcaacagc tggcggttgg gtactgacga
     1681 accgctatca ctggaaggcg ctcaggtggc atcaccggcg ctcaccgaat tacgggcaaa
     1741 cccaacggca cgcgccgcgt tatggcaaca gatttgcaca tatgaacacg atttcttccc
     1801 tcgaaacgac tgatttacca gcggcttacc acattgaaca acgcgcccac gcctttccgt
     1861 ggagtgaaaa aacctttgcc agcaaccagg gcgagcgtta tctcaacttt cagttaacgc
     1921 aaaacggcaa aatggcggcg tttgcgatta cgcaagtggt gctggatgaa gctacattgt
     1981 tcaatattgc ggtcgatcct gactatcagc gtcagggatt gggaagggtg ctgctggaac
     2041 atctgatcga cgaactggaa aaacgcggcg tggcgacact atggctggaa gtccgtgcct
     2101 caaatgctgc cgccattgcc ctgtacgaaa gtttaggctt taacgaggcg acgattcgcc
     2161 gcaattacta ccccaccacg gacggtcgcg aagacgccat catcatggcg ttgccaatca
     2221 gtatgtaaga caaggtggaa taatgaagtg ggactggatt ttctttgatg ccgatgaaac
     2281 gctgtttacc tttgactcat tcaccggcct gcagcggatg tttcttgatt acagcgtcac
     2341 ctttaccgct gaagattttc aggactatca ggccgttaac aagccactgt gggtggatta
     2401 tcaaaacggc gcgatcactt cattacagct tcagcacggg cgttttgaga gctgggccga
     2461 acggctgaaa gttgaagcag gcttgcttaa cgatgccttt attaatgcga tggcggaaat
     2521 ctgcacgccg ctgccgggcg cggtttctct gcttaacgcc attcgtggca acgccaaaat
     2581 cggcatcatc accaacggct ttagcgcctt gcagcaagtg cgtctggaac gcacgggcct
     2641 gcgtgattac ttcgatttgc tggtgatttc cgaagaagtt ggcgttgcca aaccgaataa
     2701 gaaaattttc gattatgcgc tggaacaggc gggcaatcct gaccgttcac gcgtgctgat
     2761 ggttggcgac actgccgagt ccgatattct cggtggcatc aacgccgggc ttgcgacctg
     2821 ctggctgaat gcgcacaatc gcgagcaacc agaaggcatc gcgcccacct ggaccgtttc
     2881 ttcgttgcac gaactggagc agctcctgtg taaacactga ttgcctcccc cccgttgatg
     2941 ggtaaaatag ccgcaatttt tcgttttcaa caagcgcggc gcgatgccgc ttactcaaga
     3001 agaaagaatt atgacgttgt ctccttattt gcaagaggtg gcgaagcgcc gcacttttgc
     3061 cattatttct cacccggatg ccggtaagac taccatcacc gagaaggtgc tgctgttcgg
     3121 acaggccatt cagaccgccg gtacagtaaa aggccgtggt tccaaccagc acgctaagtc
     3181 ggactggatg gagatggaaa agcagcgtgg gatctccatt actacgtctg tgatgcagtt
     3241 tccgtatcac gattgcctgg ttaacctgct cgacaccccg gggcacgaag acttctcgga
     3301 agatacctat cgtaccctga cggcggtgga ctgctgcctg atggttatcg acgctgcaaa
     3361 aggtgttgaa gatcgtaccc gtaagctgat ggaagttacc cgtctgcgcg acacgccgat
     3421 cctcaccttt atgaacaaac ttgaccgtga tatccgcgac ccgatggaac tgctcgatga
     3481 agttgagaac gagctgaaaa tcggctgcgc accgatcacc tggccgattg gctgcggcaa
     3541 gctgtttaaa ggcgtttacc acctttataa agacgaaacc tatctctatc agagcggtaa
     3601 aggccacacg attcaggaag tccgtattgt taaagggctg aataacccgg atctcgacgc
     3661 tgcggttggt gaagatctgg cacagcagct acgtgacgaa ctggaactgg tgaaaggcgc
     3721 gtctaacgag ttcgacaaag aattgttcct tgcgggcgaa atcactccgg tattcttcgg
     3781 tactgcgctg ggtaacttcg gcgttgatca tatgctggat ggcctggtgg agtgggctcc
     3841 tgcgccgatg ccgcgtcaga ctgatacccg taccgtagag gcgagcgaag acaaatttac
     3901 cggcttcgta tttaaaattc aggccaacat ggacccgaaa caccgcgacc gcgtggcgtt
     3961 tatgcgcgta gtatccggta aatatgaaaa aggcatgaag ctgcgccagg tgcgtactgc
     4021 gaaagatgtg gttatctccg acgcgctgac ctttatggcg ggcgaccgtt cgcacgttga
     4081 agaagcgtat ccgggcgata tcctcggcct gcacaaccac ggcactattc agatcggcga
     4141 cacctttacc cagggtgaga tgatgaagtt caccggtatt ccgaacttcg caccagaact
     4201 gttccgtcgt atccgcctga aagatccgct gaagcaaaaa cagctgctca aagggctggt
     4261 acagctttcc gaagagggcg cggtgcaggt gttccgtccg atctccaaca acgatttgat
     4321 cgttggtgct gttggtgtgc tgcagtttga tgtggtggta tcgcgcctga agagcgaata
     4381 cagcgttgaa gcagtatatg aatcagtcaa cgttgccact gcccgctggg tagaatgtgc
     4441 ggacgcgaag aaattcgaag agttcaagcg taagaacgaa agccaactgg cgcttgatgg
     4501 cggcgataac ctcgcttaca tcgctaccag catggtcaac ctgcgcctgg cgcaggaacg
     4561 ttatcccgac gttcagttcc accagacccg cgagcattaa ttcttgcctc cagggcgcgg
     4621 tagccgctgc gccctgtcaa tttcccttcc ttattagccg cttacggaat gttcttaaaa
     4681 cattcacttt tgcttatgtt ttcgctgata tcccgagcgg tttcaaaatt gtgatctata
     4741 tttaacaaag taatgacatt tctgacggcg ttaaataccg ttcaatgcgt agatatcagt
     4801 atctaaagcc gtcgattgtc attctaccga tattaataac tgattcagag gctgtaatgg
     4861 tcgttattca tcacccatcg cttttgtgat ggcgaccatt gacttctgta gagggtgaag
     4921 tctctcccta ttcagcaatg caacctcgtg ttgccaggct caaattacga gcaaacatac
     4981 aggaataaat cgatgactat gacaagactg aagatttcga aaactctgct ggctgtaatg
     5041 ttgacctctg ccgtcgcgac cggctctgcc tacgcggaaa acaacgcaca gactaccaat
     5101 gaaagcgcag ggcaaaaagt cgatagctct atgaataaag tcggtaattt catggatgac
     5161 agcgccatca ccgcgaaagt gaaggcggcg ctggtggatc atgacaacat caagagcacc
     5221 gatatctctg tcaaaaccga tcaaaaagtc gtgaccctga gcggtttcgt tgaaagccag
     5281 gctcaggccg aagaggcagt gaaagtggcg aaaggcgttg aaggggtgac ctctgtcagc
     5341 gacaaactgc acgttcgcga cgctaaagaa ggctctgtga agggctacgc gggtgacacc
     5401 gccaccacca gtgaaatcaa agccaaactg ctggcggacg atatcgtccc ttcccgtcat
     5461 gtgaaagttg aaaccaccga cggcgtggtt cagctctccg gtaccgtcga ttcccaggca
     5521 cagagcgacc gtgctgaaag tatcgccaaa gcggtagatg gtgtgaaaag cgttaaaaat
     5581 gatctgaaaa ctaagtaatt cgtcgtaatt cgtcctcccg aactttgtcg ggaggcgcga
     5641 tgtgcaccac actaaaaata tcgcattgag tgattttcac gctcatatca agcggtgaca
     5701 ttaactatgg taaaggagac gcttatgttt cgttggggca tcatatttct ggttatcgcg
     5761 ttaatcgccg ccgcacttgg gtttggtggt ctggccggta ccgctgcagg cgcagctaaa
     5821 attgtctttg tcgtcgggat tattctgttc ctggtgagtt tgttcatggg ccgaaaacga
     5881 ccctagattt cagaccatta agaaaatttt atccaaagcc agtccagcgg actggctttt
     5941 gcggttttag cgaataataa attgcgttac tttgtcattc tttcacaaca aaaacaggaa
     6001 ggcagaggtg gggcagcgaa tacctgtaac gcttggtaat attgcgccgt tgtcgctaag
     6061 gccgttccag cctggacgaa tagctctggt gtgcgaaggc ggcggacagc gtggaatttt
     6121 cacggctggc gtgctggatg agtttatgcg cgcgcagttt aatcctttcg atctttatct
     6181 cggcacatct gccggggcgc agaacctctc ggcgtttatc tgcaatcagc ccggttacgc
     6241 gcgcaaagtc atcatgcgct ataccacaaa acgcgaattt ttcgatccat tgcgctttgt
     6301 ccgtggagga aatcttatcg atctcgactg gctggtggag gccaccgcaa gccagatgcc
     6361 gttgcaaatg gataccgccg cgcggttgtt tgacagcggc aaatcgtttt atatgtgcgc
     6421 ctgtcgtcag gatgactacg cgcctaatta ctttttacca accaaacaaa actggctgga
     6481 tgtgattcgc gcctccagtg cgatacctgg cttttatcgt agcggagtgt cgctggaagg
     6541 cattaactac ctggatggcg ggatcagtga tgcgattccg gttaaagagg cggcaaggca
     6601 gggcgctaaa acgttggtcg tcattcgcac tgtgccgtca caaatgtact acacgccgca
     6661 gtggttcaaa cgcatggaac gctggctggg tgacagtagc ctgcagccgc tggtcaatct
     6721 ggtgcagcat catgaaacca gctatcgtga cattcagcaa tttattgaga aaccaccggg
     6781 caagctgcgg atattcgaaa tttatccgcc gaagccatta catagtatcg cgcttggcag
     6841 tcggattccg gcgctgcgtg aagactataa acttgggcgt ttatgcggtc gttatttcct
     6901 cgccacggtt ggcaagctat taactgaaaa agcgccgctt acccgccatc tggtgccagt
     6961 ggtgacgccg gaatcgatcg ttattccgcc tgcgccagtc gccaacgata cgctggttgc
     7021 cgaagtgagc gatgctccgc aggcgaacga cccgacattt aacaatgagg atctggcttg
     7081 atttgccgtt ttatcgatac ccactgccat tttgatttcc cgccgtttag tggcgatgaa
     7141 gaggccagcc tgcaacgcgc ggcacaagcg ggcgtaggca agatcattgt tccggcgaca
     7201 gaggcggaaa attttgcccg cgtgcaggcg ttagcggaaa aatatcaacc gctgtatgcc
     7261 gcattggggt tgcatccggg gatgttggaa aaacatagcg atgtgtctct tgatcagcta
     7321 cagcaggcgc tggaaaggcg tccggcgaag gtggtggcgg tgggggagat cggtctggat
     7381 ctcttcggcg acgatccgca atttgagagg cagcagtggt tactcgacga acagctgaaa
     7441 ctggcgaaac gctacgatct gccggtgatc cttcattccc ggcgtataca cgacaaactg
     7501 gcgatgcatc ttaaacgtca cgatttaccg tgcactggcg tggttcacgg tttttccggc
     7561 agcctgcaac aggccgagcg gtttgtacag ctgggctaca aaatcggcgt aggcggtact
     7621 atcacctatc cacgcgccag taaaacccgc gatgtcatcg caaaattacc gctggcatcg
     7681 ttgttgctgg aaaccgacgc gccggatatg ccgctcaacg gctttcaggg gcagcctaac
     7741 cgaccggaac aggctgtgcg ggtgtttgac gtgctttgcg agttgcgccc ggaaccagaa
     7801 gatgagattg ccgaagtgtt gcttaataat acgtatgcgt tgtttagcgt tagtgggtag
     7861 cctacccttc gtaggcagca tccggcagtc ggcgcataat gcctgatgcg acgcttaacg
     7921 cgtcttatca tgcctacagg tttgtgccga accgtaggcc ggataaggcg ttcacgccgc
     7981 atccggcagt tggcgcacaa tgcctgatgc gacgcttgac gcgtcttatc atgcctactg
     8041 gtttgtgccg aaccgtaggc cggataaggc gttcacgccg catccggcag ttggcgcaca
     8101 atgcctgatg cgacgcttga cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg
     8161 ccggataagg cgttcacgcc gcatccggca gttggcgcac aatgcctgat gcgacgctga
     8221 cgcgtcttat catgcctaca agtctgtgcc gaaccgtagg ccggataagg cgttcacgcc
     8281 gcatccggca gtcggcgcat aatgcctgat gcgacgcttg tcgcgtctta tcatgcctac
     8341 aagtctgtgc cgaaccgtag gccgtatccg gcatgtcaca aatagagcgc cggaaatatc
     8401 aaccggctca ccccgcgcac ctttaacgca tcagccaacg gctcaacgtc ttccggcgtg
     8461 gcgctcgccc agctttgcgc ctcgccatac acgccgtggg catgaaacgc attcaggcga
     8521 accggaacat cgccaagtct cttgataaac gtcgccagct catcgatgtg atgcaaataa
     8581 tccacctggt ctggaattac cagcaaacgc agttccgcca gcttgccgcg ctctgcaagc
     8641 aaacagatgc tgcgcttaat ctgctgatta tcgcgtccgg ttaagtgttg atgacattcg
     8701 ctcccccacg ctttgagatc gagcattgcg ccgtcacaca ccgggagtaa tttttcccag
     8761 ccggtttcgc tcaacatgcc gttactgtcc accaggcagg tgaggtggcg cagttgcgga
     8821 tcgtttttga tagcagtaaa cagcgccact acaaacggca gttgggtcgt ggcttcgccg
     8881 ccgcttaccg ttatcccttc aataaacagc accgctttgc ggacatggct aagcacttcg
     8941 tccacgctca tggattgcgc cattggcgtg gcatgttgcg gacacctctt caggcaggta
     9001 tcacactgct cgcaaaccac agcgttccac accactttgc cgtcaacaat ctgcaacgcc
     9061 tgatgcggac actgtggcac gcactcccca cagtcattgc aacgtcccat cgtccacgga
     9121 ttgtgacagt ttttgcagcg cagattgcag ccctgcaaaa acagagccag acgactgcct
     9181 ggcccgtcaa cgcaggagaa ggggataatc ttactgacta aagcgcatct gctgttcatg
     9241 gcttatcacg cgcggctggc gttccagaat acgagtgttg cgtgcggctt cttcgcccag
     9301 ccaggtggtg ttggtgcgtg aaccttcggc gcgatatttt tctaaatccg acaaacgcac
     9361 catataaccg gtaacgcgaa ccagatcgtt accgctgaca ttggcagtaa attcacgcat
     9421 tccggcttta aaggcaccga ggcaaagctg taccagcgcc tgcggattac gtttgatggt
     9481 ttcgtcgagc gtcagaatgt cgctgatgcc ggaataataa taagcatgat gcggggcgac
     9541 agtttgcaga tgggtgatcg gatctggctc atcaccatac ggcaaacgcg cgcccggcgt
     9601 ggtgccgata tcggaactgc tccccgactg tgcgtgtaac atggcgcgtt tttgccagcc
     9661 atatttcacg ggggtattgg cgacaaactc cgccagttgc gcgctgatgc gataacccac
     9721 ttcatttgcg gcggcttctt taccgtagcg cgcggcaatc ccttctttct tacacagcaa
     9781 gttaaccgct tccgccagcc catacatgcc aaacattggc acaaaacgtt cagggttaat
     9841 cagcccttct ttcaccagga agctattctc aaagaagtgt gattgttgat agaggaattc
     9901 acaccgcgca tcgatgatgg cgatctgctg ctggcagtag tgcggtagag tgcgcgtaaa
     9961 gaagtcatcc agcgattcgc tgcgctcggc aatggctttc aggttaaggc gtaccagcgt
    10021 gctgccacca cccgccagcg gcagtgagtt gtaacagctc acaatcccgt agcccccttt
    10081 tgtgaaaatt ttatcatgca ccggaccgtt ggcgatgtgc ggtttgctac attcacagat
    10141 gttcttcgcc acttccagca gcaggtcatc aggggtgatt tcaggatcgt agataaaggt
    10201 caggttcggt gaaacctgct tcaactctgc gtctgcacgt aagatcgcgc gggtaatggg
    10261 cgaatcagac gggccgatat tggcgtgcat aaaggcgtct ggcagggttc tgtcgaggta
    10321 acgccagaaa cgttttattc gaacatcgat ctcgtcttgt gttagaattc taacatatgg
    10381 ttgcaacaac gcatccagtt gccccaggta gaccggcatc gatgtgaccg acggtacgtg
    10441 gtggtaaaga atggtcagca gagagagtgc gtcatcaaga tctttcgcgc cttccagctc
    10501 cagccattcg gaaccgttcg ccagaaaacg ggcgtaatcg ggtaagacat agcgcggttt
    10561 gtacggcgca tgaccttcaa acatatcgca gattacaccc tcatccagcg cgcggcgggc
    10621 ttcggcagga agctgcggat aaggcagatt gttttctgct tccagtgcca gaaaatggcg
    10681 cttctgctcc gggctaagca ctgggctggt gacaatttgc tggcaacgtt gttgcagtgc
    10741 attttcatga gaagtgggca tcttcttttc cttttatgcc gaaggtgatg cgccattgta
    10801 agaagtttcg tgatgttcac tttgatcctg atgcgtttgc caccactgac gcattcattt
    10861 gaaagtgaat tatttgaacc agatcgcatt acagtgatgc aaacttgtaa gtagatttcc
    10921 ttaattgtga tgtatatcga agtgtgttgc ggagtagatg ttagaatact aacaaactcg
    10981 caaggtgaat tttattggcg acaagccagg agaatgaaat gactgatctg aaagcaagca
    11041 gcctgcgtgc actgaaattg atggacctga ccaccctgaa tgacgacgac accgacgaga
    11101 aagtaattgc tctgtgtcat caggccaaaa ccccggtcgg caataccgcc gctatctgta
    11161 tctatcctcg ctttatcccg attgctcgca aaacgctgaa agagcagggc accccggaaa
    11221 tccgaatcgc tacggtaacc aacttcccac acggtaacga tgacatcgaa atcgcgctgg
    11281 cagaaacccg tgcggcaatc gcctacggtg ctgatgaagt tgacgttgtg ttcccgtacc
    11341 gcgcgctgat ggcgggtaac gagcaggttg gttttgacct ggtgaaagcc tgtaaagagg
    11401 cttgcgcggc agcgaatgta ctgctgaaag tgatcatcga aaccggcgaa ctgaaagacg
    11461 aagcgctgat ccgtaaagcg tctgaaatct ccatcaaagc gggtgcggac ttcatcaaaa
    11521 cctctaccgg taaagtggct gtgaacgcga cgccggaaag cgcgcgcatc atgatggaag
    11581 tgatccgtga tatgggcgta gaaaaaaccg ttggtttcaa accagcgggc ggcgtgcgta
    11641 ctgcggaaga tgcgcagaaa tatctcgcca tcgcagatga actgttcggt gctgactggg
    11701 cagatgcgcg tcactaccgc tttggtgctt ccagcctgct ggcaagcctg ttgaaagcgc
    11761 tgggccacgg tgacggtaag agcgccagca gctactaagt aagatgcttt acgcctgatg
    11821 cgctgcgctt atcaggccta cgagacgtat ctacccgtag gccggataag gcgtagacgc
    11881 atccggcaaa agccgcctca tactcttttc ctcgggaggt taccttgttt ctcgcacaag
    11941 aaattattcg taaaaaacgt gatggtcatg cactgagcga tgaagaaatt cgtttcttta
    12001 tcaacggcat tcgcgacaac actatctccg aagggcagat tgccgccctc gcgatgacca
    12061 ttttcttcca cgatatgaca atgcctgagc gtgtctcgct gaccatggcg atgcgagatt
    12121 caggaaccgt tctcgactgg aaaagcctgc atctgaatgg cccgattgtt gataaacatt
    12181 ccactggtgg cgtcggcgat gtgacttcgc tgatgttggg gccgatggtc gcagcctgcg
    12241 gcggctatat tccgatgatc tctggtcgcg gcctcggtca tactggcggt acgctcgaca
    12301 aactggaatc catccctggc ttcgacattt tcccggatga caaccgtttc cgcgaaatta
    12361 ttaaagacgt cggcgtggcg attatcggtc agaccagctc actggctccg gcggataaac
    12421 gtttctacgc gacccgtgat attaccgcaa ccgtggactc catcccgctg atcaccgcct
    12481 ctattctggc gaagaaactt gcggaaggtc tggacgcgct ggtgatggac gtgaaagtgg
    12541 gtagcggcgc gtttatgccg acctacgaac tctctgaagc ccttgccgaa gcgattgttg
    12601 gcgtggctaa cggcgctggc gtgcgcacca ccgcgctgct caccgacatg aatcaggtac
    12661 tggcctccag tgcaggtaac gcggttgaag ttcgtgaagc ggtgcagttc ctgacgggtg
    12721 agtatcgtaa cccgcgtctg tttgatgtca cgatggcgct gtgcgtggag atgcttatct
    12781 ccggcaaact ggcgaaagat gacgccgaag cgcgcgcgaa attgcaagca gtgctggaca
    12841 acggtaaagc ggcagaagtc tttggtcgta tggtagcggc acaaaaaggc ccaaccgact
    12901 tcgttgagaa ctacgcgaag tatctgccga cagcgatgct gacgaaagca gtctatgctg
    12961 ataccgaagg ttttgtcagt gaaatggata cccgcgcgct ggggatggca gtggttgcaa
    13021 tgggcggcgg tcgtcgtcag gcatctgaca ccattgatta cagcgtcggc tttactgata
    13081 tggcgcgttt gggcgaccag gtagacggtc agcgtccgct ggcggttatc cacgcgaaag
    13141 acgaaaacag ctggcaggaa gcggcgaaag cggtgaaagc ggcaattaaa cttgccgata
    13201 aagcaccgga aagcacacca actgtctatc gccgtatcag cgaataacgg tatactgatc
    13261 tgatcattta aatttgaagc actgagtacg gagaacatat gaaacgtgca tttattatgg
    13321 tgctggactc attcggcatc ggcgctacag aagatgcaga acgctttggt gacgtcgggg
    13381 ctgacaccct gggtcatatc gcagaagctt gtgccaaagg cgaagctgat aacggtcgta
    13441 aaggtccgct caatctgcca aatctgaccc gtctggggct ggcgaaagca cacgaaggtt
    13501 ctaccggttt cattccggcg ggaatggacg gcaacgctga agttatcggc gcgtacgcat
    13561 gggcgcacga aatgtcatcc ggtaaagata ccccgtctgg tcactgggaa atagccggtg
    13621 tcccggttct atttgagtgg ggatatttct ccgatcacga aaacagcttc ccgcaagagc
    13681 tgctggataa actggttgaa cgcgctaatc tgccgggtta cctcggtaac tgccactctt
    13741 ccggtacggt cattctggat cagctgggcg aagagcacat gaaaaccggc aagccgattt
    13801 tctatacctc cgctgactct gtgttccaga ttgcctgcca cgaagaaacc tttggcctgg
    13861 ataaactcta cgaactgtgc gaaatcgccc gtgaagagct gaccaacggc ggctacaaca
    13921 tcggtcgtgt tatcgctcgt ccgtttatcg gtgacaaagc cggtaacttc cagcgtaccg
    13981 gtaaccgtca cgacctggct gttgagccgc cagcaccgac cgtgctgcag aaactggttg
    14041 atgaaaaaca cggtcaggtg gtttctgtcg gtaaaattgc agacatctac gccaactgcg
    14101 gtatcaccaa gaaagtgaaa gcaactggcc tggacgcgct gtttgacgcc accatcaaag
    14161 agatgaaaga agcgggtgat aacaccatcg tcttcaccaa cttcgttgac ttcgactctt
    14221 cctggggcca ccgtcgcgac gtcgccggtt atgctgtggg tctggagctg ttcgaccgcc
    14281 gtctgccgga gctgatgtct ctgctgcgcg atgacgacat cctgatcctc actgctgacc
    14341 acggttgtga tccgacctgg accggtactg accacacgcg tgaacacatt ccggtactgg
    14401 tttacggccc gaaagtaaaa ccgggctcac tgggtcaccg tgaaaccttc gcggatatcg
    14461 gccagactct ggcaaaatat tttggtactt ctgatatgga atatggcaaa gccatgttct
    14521 gataatgggt gcggcttgat gccctcaccc cggccctctc cctcagggag agggtgaaaa
    14581 catgttgata aggataaaac aatggctacc ccacacatta atgcagaaat gggcgatttc
    14641 gctgacgtag ttttgatgcc aggcgacccg ctgcgtgcga agtatattgc tgaaactttc
    14701 cttgaagatg cccgtgaagt gaacaacgtt cgcggtatgc tgggcttcac cggtacttac
    14761 aaaggccgca aaatttccgt aatgggtcac ggtatgggta tcccgtcctg ctccatctac
    14821 accaaagaac tgatcaccga tttcggcgtg aagaaaatta tccgcgtggg ttcctgtggc
    14881 gcagttctgc cgcacgtaaa actgcgcgac gtcgttatcg gtatgggtgc ctgcaccgat
    14941 tccaaagtta accgcatccg ttttaaagat catgactttg ccgctatcgc tgactttgac
    15001 atggtgcgta acgcggtaga cgcggctaaa gcactgggca ttgatgctcg cgttggtaac
    15061 ctgttctccg ctgacctgtt ctactctccg gacggcgaaa tgttcgacgt gatggaaaaa
    15121 tacggcatcc tcggcgtgga aatggaagcg gctggtatct acggcgtcgc tgcagaattt
    15181 ggcgcgaaag ccctgaccat ctgcactgtg tctgaccaca tccgcactca cgagcagacc
    15241 actgccgctg agcgtcagac caccttcaac gacatgatca aaatcgcact ggaatccgtt
    15301 ctgctgggcg ataaagagta attgtgtttc gctgaaaggc gattgtcg
//
LOCUS       DAEEKQ010000092        15236 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.092, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000092 DAEEKQ010000000
VERSION     DAEEKQ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15236
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.092"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..749
                     /locus_tag="KLK57_002687"
     CDS             <1..749
                     /locus_tag="KLK57_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF945 family protein"
                     /protein_id="HBE0893016.1"
                     /translation="SKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYEL
                     DKLTKGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAVHPELANDEVALQEV
                     NAALFKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKLLN
                     FDVKLPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASL
                     QLRYTPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(790..1722)
                     /locus_tag="KLK57_002688"
     CDS             complement(790..1722)
                     /locus_tag="KLK57_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HBE0893017.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            2086..4872
                     /gene="polA"
                     /locus_tag="KLK57_002689"
     CDS             2086..4872
                     /gene="polA"
                     /locus_tag="KLK57_002689"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HBE0893018.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            complement(5254..5886)
                     /locus_tag="KLK57_002690"
     CDS             complement(5254..5886)
                     /locus_tag="KLK57_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HBE0893019.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            6468..6977
                     /gene="yihI"
                     /locus_tag="KLK57_002691"
     CDS             6468..6977
                     /gene="yihI"
                     /locus_tag="KLK57_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HBE0893020.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVAEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            7166..8539
                     /gene="hemN"
                     /locus_tag="KLK57_002692"
     CDS             7166..8539
                     /gene="hemN"
                     /locus_tag="KLK57_002692"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HBE0893021.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(8729..8839)
                     /locus_tag="KLK57_002693"
     CDS             complement(8729..8839)
                     /locus_tag="KLK57_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HBE0893022.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="KLK57_002694"
     CDS             complement(8951..10360)
                     /gene="glnG"
                     /locus_tag="KLK57_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HBE0893023.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="KLK57_002695"
     CDS             complement(10372..11421)
                     /gene="glnL"
                     /locus_tag="KLK57_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HBE0893024.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="KLK57_002696"
     CDS             complement(11595..13004)
                     /gene="glnA"
                     /locus_tag="KLK57_002696"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HBE0893025.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            13377..15200
                     /gene="typA"
                     /locus_tag="KLK57_002697"
     CDS             13377..15200
                     /gene="typA"
                     /locus_tag="KLK57_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HBE0893026.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
BASE COUNT         3965 a         3803 c         3915 g         3553 t
ORIGIN      
        1 agtcgaaatt taatcagatt aacattatca atcacgggga agacgttgcc caaatcgatg
       61 ctttcgttgc aaaaaccatg ctggatcgcg ttaaagacaa agattatatc aatgtcaatc
      121 tgacctacga acttgataag ttaacaaaag ggaatcagca actcggtagt ggtgagtggt
      181 cattgattgc tgaatctatt gatccctcag cagtgcgcca atttatcatc cagtataaca
      241 ttgctatgca gaagcagctt gctgtacacc ctgagttagc aaacgatgaa gttgctctgc
      301 aagaagtgaa tgctgcattg ttcaaagagt atttaccgtt attacaaaaa agtgagccga
      361 ccattaaaca accggtaaga tggaagaacg cactcggcga actaaatgcc aatctggata
      421 tcagtattgc cgacccagcc aaatcttcat catccacaaa caaagatatc aaattgctca
      481 attttgatgt gaagttaccg cttaatgtcg tcacagaaac cgcaaaacag cttaatttat
      541 ctgaaggaat ggatgcggaa aaagcgcaaa agcaggctga taaacaaatc agcgggatga
      601 tgacattggg tcagatgttt cagttaatca cgattgacaa caataccgcc tcgctgcagc
      661 tgcgttatac accgggtaaa gttgtgttta acggacagga gatgagcgaa gaagaattta
      721 tgtctcgtgc cggacgtttt gttcattaaa atactcactc gcctctgctc attcagaggc
      781 gagtaaaact tacttattct gacgctgtga tgacatgaga gacgtcagaa gccggtcttt
      841 ttcctgccac aatgagttca gccattgctg aaaatggcgc ttaaagcttt tgtcattgat
      901 gtagtcgcca tgtaactcat cggcaatagg ctgtaaatcc acatgtacga caatccgcgt
      961 cagtttaccg cttaacatat cgaagaacgg ctgacggtta ttgtccggat aacacagcgt
     1021 aacattaagc agtttatcga attgtttacc cagtacattt agcgccatcg caatgcctgc
     1081 cgcctttggt ggcaacaagt tttgaaaagt ggagtgggtt tgctgatgtt tttcttgcgt
     1141 gaaacgggag ccttcaacga aattgacaat ggtggtggga tgcaggcgaa acttttcaca
     1201 agaacgccga gtggtttcaa catctttacc acgtcgctcc ggatgacgta ataaataagc
     1261 gcgggaataa cgcttcataa acggcatatc cagcgcccag cacgccaggc caagaaatgg
     1321 cacccaggcc agctgttgct tgaggaaata tttattcatc ggaatgtgct tacgaaacag
     1381 cacgcacagt acgacaatat ctgcccaact acggtggtta caaataagca gataccagtt
     1441 cttcttactt aacccttcca gcccgtgaac ttcccattgc aggtgtgggt tcagatgcag
     1501 taataccgcc agaccttcac accagcaata catcataaaa tcacaaaaac gcgaaacctt
     1561 tcgccaaatg actggcacag gtagcaatag ttttacaatc ccggcaatga tgatcgggac
     1621 ggaacaaaaa atagtgacca aaatggtcaa tacgatactc agcaaaaggg ttatcgcagc
     1681 gagtattctc gtcataatga atttattcaa aaggttagcc atagacagtg cgctaaagaa
     1741 gcgcaaggcg cagattttag cagaaaacga cccaaataac ggatgatcct taaggagaaa
     1801 aataatccat atctatccac attagaaaaa atcccattat ctcaattatt agggatggct
     1861 ttatttttaa ctgcatgaaa aacaaagaca aacatcatgc tgtaaaaagc atgataataa
     1921 attaaaagcg atgtaaataa tttatgcaca aagttatcca catgacgatt tgcgagcgat
     1981 ccagaagatc tacaaaagat tttcacgaaa agcggtgaaa aactcatgtt ttcatcctgt
     2041 ctgtggcatc ctttacccat aatctgataa acaggcacgg acattatggt tcagatcccc
     2101 caaaatccac ttatccttgt agatggttca tcttatcttt atcgcgcata tcacgcgttt
     2161 cccccgctga ctaacagcgc aggcgagccg accggtgcga tgtatggtgt cctcaacatg
     2221 ctgcgcagtc tgatcatgca atataaaccg acgcatgcag cggtggtctt tgacgccaag
     2281 ggaaaaacct ttcgtgatga actgtttgaa cattacaaat cacatcgccc gccaatgccg
     2341 gacgatctgc gagcacaaat cgaacccctg cacgcgatgg ttaaagcgat gggactgccg
     2401 ctgctggcgg tttctggcgt agaagcggac gacgttatcg gtactctggc gcgcgaagcc
     2461 gaaaaagccg ggcgtccggt gctgatcagc actggcgata aagatatggc gcagctggtg
     2521 acgccaaata ttacgcttat caacaccatg acgaatacca tcctcggacc ggaagaggtg
     2581 gtgaataagt acggcgtgcc gccagaactg atcatcgatt tcctggcgct gatgggtgac
     2641 tcctccgata acattcctgg cgtaccgggc gtcggtgaaa aaaccgcgca ggcattgctg
     2701 caaggtcttg gcgggctgga tacgctgtat gccgagccag aaaaaattgc tgggttgagc
     2761 ttccgtggcg cgaaaacaat ggcagcgaag ctcgagcaaa acaaagaagt tgcttatctc
     2821 tcataccagc tggcgacgat taaaaccgac gttgaactgg agctgacctg tgaacaactg
     2881 gaagtgcagc aaccggcagc ggaagagttg ttggggctgt tcaaaaagta tgagttcaaa
     2941 cgctggactg ctgatgtcga agcgggcaaa tggttacagg ccaaaggggc aaaaccagcc
     3001 gcgaagccgc aggaaaccag tgttgcagac gaagcgccag aagtgacggc aacggtgatt
     3061 tcttatgaca actacgtcac catccttgat gaagaaacac tgaaagcgtg gattgcgaag
     3121 ctggaaaaag cgccggtatt tgcatttgat accgaaaccg acagccttga taacatctct
     3181 gctaacctgg tcgggctttc ttttgctatc gagccaggcg tagcggcata tattccggtt
     3241 gctcatgatt atcttgatgc gcccgatcaa atctctcgcg agcgtgcact cgagttgcta
     3301 aaaccgctgc tggaagatga aaaggcgctg aaggtcgggc aaaacctgaa atacgatcgc
     3361 ggtattctgg cgaattacgg cattgagctg cgtgggattg cgtttgatac catgctggag
     3421 tcctacattc tcaatagcgt tgccgggcgt cacgatatgg acagcctcgc ggaacgttgg
     3481 ttgaagcaca aaactatcac ttttgaagag attgcgggta aaggcaaaaa tcaactgacc
     3541 tttaaccaga ttgccctcga agaggccgga cgttacgccg ccgaagatgc ggatgtcacc
     3601 ttgcagttgc atctgaaaat gtggccagat ctgcaaaaac acaaagggcc gttgaacgtc
     3661 ttcgagaata tcgaaatgcc gctggtgccg gtgctttcac gcattgaacg taacggtgtg
     3721 aagatcgatc cgaaagtgct gcacaatcat tctgaagagc tcacccttcg tctggctgag
     3781 ctggaaaaga aagcgcatga aattgcaggt gaggaattta acctttcttc caccaagcag
     3841 ttacaaacca ttctgtttga aaaacagggc attaaaccgc tgaagaaaac gccgggtggc
     3901 gcgccgtcaa cgtcggaaga ggtactggaa gaactggcgc tggactatcc gttgccaaaa
     3961 gtgattctgg agtatcgtgg tctggcgaag ctgaaatcga cctacaccga caagctgccg
     4021 ctgatgatca acccgaaaac cgggcgtgtg catacctctt atcaccaggc agtaactgca
     4081 acgggacgtt tatcgtcaac cgatcctaac ctgcaaaaca ttccggtgcg taacgaagaa
     4141 ggtcgtcgta tccgccaggc gtttattgcg ccagaggatt atgtgattgt ctcagcggac
     4201 tactcgcaga ttgaactgcg cattatggcg catctttcgc gtgacaaagg cttgctgacc
     4261 gcattcgcgg aaggaaaaga tatccaccgg gcaacggcgg cagaagtgtt tggtttgcca
     4321 ctggaaaccg tcaccagcga gcaacgccgt agcgcgaaag cgatcaactt tggtctgatt
     4381 tatggcatga gtgctttcgg tctggcgcgg caattgaaca ttccacgtaa agaagcgcag
     4441 aagtacatgg acctttactt cgaacgctac cctggcgtgc tggagtatat ggaacgcacc
     4501 cgtgctcagg cgaaagagca gggctacgtt gaaacgctgg acggacgccg tctgtatctg
     4561 ccggatatca aatccagcaa tggtgctcgt cgtgcagcgg ctgaacgtgc agccattaac
     4621 gcgccaatgc agggaaccgc cgccgacatt atcaaacggg cgatgattgc cgttgatgcg
     4681 tggttacagg ctgagcaacc gcgtgtacgt atgatcatgc aggtacacga tgaactggta
     4741 tttgaagttc ataaagatga tgttgatgcc gtcgcgaagc agattcatca actgatggaa
     4801 aactgtaccc gtctggatgt gccgttgctg gtggaagtgg ggagtggcga aaactgggat
     4861 caggcgcact aagattcgcc tgaacatgcc cttttttcgt aagtaagcaa cataagctgt
     4921 cacgttttgt gatggctatt agaaattcct atgcaacaac tgaaaaaaaa ttacaaaaag
     4981 tgctttctga actgaacaaa aaagagtaaa gttagtcgcg tagggtacag aggtaagatg
     5041 ttctatcttt cagacctttt acttcacgta atcggatttg gctgaatatt ttagccgccc
     5101 cagtcagtaa tgactggggc gttttttatt gggcgaaaga aaagatccgt aatgcctgat
     5161 gcgctatgtt tatcaggcca acggtagaat tgtaatctat tgaatttacg ggccggatac
     5221 gccacatccg gcacaagcat taaggcaaga aaattattcg ccgtcctgcg tttcttctac
     5281 aggctgcatc tcgctaaacc aggtatccag tttctgccgc agcttgtcca cgccttgttt
     5341 cttcaacgaa gaaaacgttt caacctgcac atcaccgtta aacgccagta cagcttcacg
     5401 caccatattc aattgcgctt tacgtgcgcc gcttgccagt ttgtccgctt tggtcagcag
     5461 caccagaacg gcgatattgc tgtctaccgc ccactcaatc atctgctgat ccaaatcttt
     5521 cagcggatgg cgaatatcca ttagcaccac cagaccttgc aggctctgac gtttttcgag
     5581 gtattcgccg agcgcacgct gccatttgcg cttcatctct tccgggactt ccgcataacc
     5641 gtacccaggc aagtcaacca gacgcttgcc gtcagccact tcaaacaggt tgataagctg
     5701 ggtgcgccct ggggtttttg aggtacgagc caggcttttc tggttagtca gcgtgttcag
     5761 cgcgctggat ttacctgcgt tggaacggcc tgcaaaagcc acttcaattc cggtatcgga
     5821 aggtaggtgg cgaatatcag gcgcactcat cacaaaatgc gtctgttgat aattcaaatt
     5881 agtcaaagcg gtcgtctccg tcagtcaaag ctttgtggcg attatacctg aaccataata
     5941 aaaggcggat ttttcggcgt gagcgttgta agtaaaagcc atacgctttg tgagacattg
     6001 ccgatagtct ttatgcgaaa tagcagagaa aattctgcga tgcatgtcaa ataagctata
     6061 taaatcagtg aattgctttt atataaaact ctgaaaatgt gcaaatactg atggcggttg
     6121 attgtttgtt taaagcaaag gcgtaaagta gcacccatag agcgaggacg ctaacaggaa
     6181 caatgactca ggatgagggt caggagcgcc aggaggcgaa gacagaggat tgtcaggaag
     6241 acaaacgtcc ggagacgtaa ttaaacggaa atggaatcaa cacggattgt tccggctaaa
     6301 ggaaaaacag ggtgtgttgg cggcctgcaa ggattgtaag acccgttaag ggttatgagt
     6361 caggaaaaaa ggcgacagag taatctgtcg ccttttttct ttgcttgctt tctgttagat
     6421 tccgccgcaa atctatactg aataaaacgg ctaaaagacg aaccattatg aaaccatcat
     6481 cttcaaactc acgcagcaaa ggtcatgcaa aagcgcgtcg aaaaacacgc gaggagctgg
     6541 atcaggaagc tcgtgatcgc aagcgtcaga aaaaacgtcg tggtcatgcg ccgggcagcc
     6601 gcgcagcggg cggaaacacc acgtcaggca gcaaaggcca gaacgcacca aaagatccac
     6661 gtattggcag taaaacccct attccattgg gcgtggctga aaaagtcacc aaacagcaca
     6721 aaccgaagag tgagaaacct atgctttcac cgcaggcgga gttggaatta ctggaaacgg
     6781 atgagcgtct ggatgcgctg ctggaacgtc tggaagcagg cgaaaccctg agcgccgaag
     6841 agcaatcctg ggtggatgct aaactggatc gtattgatga gttgatgcag aaactcggcc
     6901 tctcttatga cgatgacgaa gaagaggaag aagacgagaa gcaagaagac atgatgcgtc
     6961 tgctgcgggg taactaacgg attgccaccg tgggccttcc cgttctgctg ataacccttc
     7021 cggttatatg ttatctggtg tggttattcg ttaaactaca gcggttgtcg cggcgacaaa
     7081 agtggctgcg caaccggctg atgacccgaa acgggcatcc gccggtacgc cgtagccgcc
     7141 agagacgcca tcggaaggag tgagcatgtc tgtacagcaa atcgactggg atctggccct
     7201 gatccagaaa tataactatt ccgggccacg atacacctcg tacccgaccg cgctggagtt
     7261 ttcagaagac ttcggcgaac aggcgttttt acaagccgtg gcgcgctatc ctgagcgtcc
     7321 attatctctc tacgtacata tcccgttctg ccataagctt tgttacttct gcggttgcaa
     7381 taagattgtt actcgccagc agcacaaggc cgatcagtat ctggacgcgc tggagcaaga
     7441 aatcgtccat cgtgcaccgc tgttcgccgg gcgtcacgtc agccaattgc actggggcgg
     7501 cggaacgccg acgtatctga ataaagcgca aatcagccgc ctgatgaagc tgctgcgcga
     7561 aaacttccag ttcaatgccg atgcggagat ttcgatcgaa gtcgatccgc gggaaatcga
     7621 actggatgta ctcgatcatt tacgcgccga aggctttaat cgcctgagca tgggcgtgca
     7681 ggacttcaac aaagaagtgc aacgtctggt taaccgcgag caggatgaag agttcatctt
     7741 tgcactgctt aaccatgcgc gtgagattgg ttttacctcc accaacatcg acctgattta
     7801 cggcctgccg aaacagacgc cggagagttt cgcctttacc ctgaaacgtg tggcggagct
     7861 gaaccccgat cgtctgagtg tctttaacta cgcgcatctg ccgaccattt ttgctgctca
     7921 gcgcaaaatc aaagatgctg acctgccgag tccgcagcaa aaactcgata tcctgcagga
     7981 aaccatcgcc ttcctgacgc aatcgggcta tcagtttatc ggtatggatc actttgcccg
     8041 tccggatgac gagctggcgg tggcccagcg tgaaggcgtg ctgcatcgta acttccaggg
     8101 ctacaccact cagggcgata ccgatctgct ggggatgggc gtttccgcca tcagcatgat
     8161 tggcgactgc tacgcgcaga accagaaaga gttgaagcag tactatcagc aagtggatga
     8221 acaaggcaat gctctgtggc gtggtattgc gctaacgcgt gatgactgta ttcgccgcga
     8281 tgtgattaag tcgctcatct gcaacttccg tctggattac gcccctattg agaaacagtg
     8341 ggatttgcac ttcgctgatt actttgcgga agatctcaag ctgctcgccc cgttagcaaa
     8401 agatgggctg gtggatgtgg atgagaaggg aatacaggtg acggcgaaag gtcgcttgct
     8461 gatccgcaac atttgcatgt gctttgatac ctatctgcgc cagaaagcgc ggatgcagca
     8521 gttctcacgg gtgatttaaa tagtgaatgg cgcttcgttt acaaagtaga aaacgaaagc
     8581 gccatcaatg tgctgagaac aagattgcct gatgcgctac gcttatcagg cctacaaaac
     8641 ctgttgaaaa tattgctttt gtaggccgga taaggcgttc acgccgcatc cggcatgaac
     8701 aaagagcact tcatcagcaa tctgaaactc aactaaacag cccaatcatt gcggcacaca
     8761 gcacggcagc aacggcagtt tgcggtgtgt ggctgtcaac tgcgccactc gataccagat
     8821 taattattga ttccagcata atgactctcc ccgttttccg ggcaagatca tactgaactt
     8881 atcggaacag taaagcgtaa aataccagca atttgcgctc aataatcaat ctttacacac
     8941 aagctgtgaa tcactccatc cccagctctt ttaacttacg cgtcagggtg ttgcggcccc
     9001 agccgagtag ccgcgccgct tcctgtttat gcccctgcgt atgtcgtaac gcggtcgtca
     9061 gtaacgtccg ctccagctct ggctgcgctt cggaaagcag attttgatga ccggaacgca
     9121 gcgctctgtc tgcccactgc gcaagaagcg ttgcccagct gtccggttgc atttgcgaag
     9181 tactctccgc aaccgttgat tcaaacagtt cgccgggcaa atcctgaatc aacacttcct
     9241 gcccggcggc catcaccgtt agccagcggc aggtgttttc cagctggcgc acgttgcctg
     9301 gccacgccag acgcgtcaga gcagcttcgg tttccggatg cagtaacttc gcttctacgc
     9361 ccagttcgcg cgcggcaacc tgtaaaaaat ggcgcgccag acggggaata tcttcccgac
     9421 gttcgcgcag cggcggcaga tgaacgcgga taacgttcag gcggtggaac agatcctcac
     9481 ggaacttacc ttcctgcact cgctgttcga gattctggtg agtggcagcg ataatacgca
     9541 catccacttt caccggcgca tagccgccaa cgcggtaaaa ctgaccgtct gccagcacgc
     9601 gcagcaaacg cgtctgcaca tccagcggca tatcaccaat ttcgtcgagg aataatgtac
     9661 cgccatcggc ctgttcaaaa cgcccctgac gaatggtatt cgcgccagta aacgcgcctt
     9721 tctcgtggcc aaacagttct gattcgatca aatcttttgg gatagctgcc atattcagcg
     9781 cgataaacgg cgctttggcg cgcggactgt ggcgatgcag ggcatgagcg accagttctt
     9841 taccggtgcc ggattcgccg ttaatcagca cgctaataga agaacgcgaa agccgaccga
     9901 taatacggaa cacgtcctgc atggctggcg cttcgccgat gatatcggtc gttgggccgt
     9961 taagctgaat attacgcggc tgctgctgtt cctggtaatg actgatagcg cgctcaacca
    10021 gcgccactgc ttcgtcgata tcaaacggtt tgggcagata atcaaacgcc ccttgttgat
    10081 aggcgctgac ggcagcatcc agatcggaat gtgcggtcat aatgatgacc ggaagcattg
    10141 gatggcgctg tttaatctgc ttgagcagcg ccagcccgtc cattcccggc atacggatat
    10201 ctgaaagcag cacatccggc gttttgctcg ccagcgcctc cagtacttcc gcgccgttct
    10261 caaatgtcgt acaggttaaa cccgctccag cgagcgcacg ttcaagcacc caacggatgg
    10321 aactatcgtc atcgactacc cagactatcc ctcgttgcat aaacgtcacc tttatttcct
    10381 gataggcagg taaaccgaga actcggtatg acctggccaa ctggtaaatt caattttgcc
    10441 tgaatgctga tcaatcaaat tacgggcgat ggataagcca agcccggtgc caccttcgcg
    10501 gccgctgacc atcgggtaaa acagcgtatc ctgcaaatga ggcggaatgc ccggcccgtt
    10561 atcttccaca tcaatccgcg ccgccagccg gtagcgctcg ccgtgtaagg tcagttgaaa
    10621 cgcggtgcgg gtacgcagaa tgatttcacc gccttccggc cccagcgcct gtagcgcatt
    10681 gcgcacaata ttcagcaaga cctgttcaat ttgatccggg tcgtgcgcca gttccggcag
    10741 gctggggtcg taatcacgaa tcaaccgcac gttgtccggc agttccatcg acaccagcgt
    10801 caccacgcgt tcagctactt tgtgaatact ttcggtaacg cgcgtaccgg gcagctgcgg
    10861 ccccaacaga cggtcgacca gatttcgcag ccggtccgcc tgttcgataa tcactttggt
    10921 atattcgagt agtgatgggt caggtaacgc tttgctgagc agctgcgccg cgccacgtaa
    10981 acctccaagc ggatttttaa tctcatgtgc caggccgcgc actaaatcac gggcagcaac
    11041 ctgctgggcg tgctgtagct gttcctgact taagcggcgc tggttatcca tcggagccat
    11101 ctccagcagg atcatgccgt ccggcatacg ctgggccgtc acagaaagga tatgcgagcg
    11161 cccgtcgatg accagcgtca cttcgttatc ggtaaaacct tgccccgcct ccagactttc
    11221 ttgcatcagc tcgatattta atgagaagta gctcaacagt tccggtaacg gtgtaccaaa
    11281 caatttgcgg gagctttggg cgagcagttg ttgcgcggcg gggttggcgt aatggatcgc
    11341 cagattgtca tcaattaaca aaatactgtt aatcagcgag ttgaggatct gcccagcatc
    11401 gggctgcgtg cctgttgcca taaagcagtc tcctgaacag gttgcaccat tttagtgcat
    11461 tatagctttt tacggataaa aagcgcgaag catcagagaa ttgacggaga aaaaagccca
    11521 tgcagagatg ggctgaaagt ttccacggca actaaatccc ggcgttgttg cgccgggtag
    11581 tacaacttca actattagac gctgtagtac agctcaaact ctaccggatg cggagtcata
    11641 cgcacgcggt catcttcttc gcgacgtaga gcgatgtacg catcaattgc ttcgtcagtg
    11701 aacacgccac cggctttcag gaactcgcgg tccagatcca gttcgttcag tgcttcttcc
    11761 agagagcctg caacctgtgg gatctctttc gcttcttctg gcggcaggtc atacaggttt
    11821 ttgtccatgg cttcgcccgg atggatcttg ttcttgatac catcaagacc ggccatcagc
    11881 agggcagcaa agcacaggta cgggttagct gccggatccg ggaaacgtac ttcgatacga
    11941 cgtgctttcg gagaagaaac caccggaata cggatagacg cagaacggtt acgcgcagag
    12001 taagccagca ttaccggtgc ttcatagccc gggaccagac gcttataaga gttggtggtc
    12061 gggtttgcca gggcgttaat cgctttagcg tgtttgatta cgccgccaat gtagtacagc
    12121 gcctgctcag acagacctgc gtatttgtcg cctgcgaaca ggttaacgcc gtttttagac
    12181 agagacatgt ggcagtgcat accggagccg ttatcaccga acatcggttt tggcataaag
    12241 gtcgcggttt taccgaagcg gtgcgctacg ttgtgcacaa catatttgta gatctgaatt
    12301 tcgtcagctt ttttggtcat agtattgaag cgggtagcca cttcgttctg accagcagtc
    12361 gctacttcgt ggtgatgggc ttcaaccacc agacccatct gttccatcac cagacacatt
    12421 tcagaacgaa tatcctgagc cgagtctacc ggtggaaccg ggaagtaacc gcctttcact
    12481 gccggacggt gacctttgtt accaccttcg tattgggtgg aggagttcca tgcgccttcg
    12541 atatcgtcga tagcaacgtg ggaaccggag atagatgatc cgaaacggat gtcatcgaac
    12601 aggaagaatt caggttctgg cccgaacagt acggtgtcgg caatgccagt ggaacgcagg
    12661 taatcttcgg cgcgcttcgc aatggagcgc gggtcacggt catagccttg cagggtgcca
    12721 ggttcaagga tgtcgcaacg gataatcagg gtggagtcgg cgaagaacgg gtcaatcact
    12781 gcggtggatg cgtctggcat cagcaccatg tcggactcgt taatgccttt ccagccgcca
    12841 atcgaggagc cgtcaaacat tttgccttct tcgaagaatt cagcattcac ctgatgagca
    12901 gggatagtga cgtgctgttc tttaccttta gtatcggtga agcgcaaatc aacaaacttc
    12961 acttcgtgct cgttcagcat cgtcagtacg tgttcagcgg acatacttta actctcctgg
    13021 attggtcatg gtcgtcgtgg taacgaaatc tgcaattatt ttggccgtgt cgccgtaaaa
    13081 aagataaagc gaaatctgtg ccaactttta aattgcccct aaaaggcgtt atcatgcgca
    13141 ccatcgtgca aaagggctgc accacgatgt gaatgttgca ccaatatagt gcttcaatgg
    13201 aaacattaag caccatgttg gtgcaatgac ctttggataa ccctttttat gctccgtgaa
    13261 agcgatcaca aagggactct gcaatacttg tttgcggagg atgtttgtga tcctgttttg
    13321 tagtgcgatt aatccgtgta caataacgcg ctatttctaa tgcctgaggc aaagttgtga
    13381 tcgaaaaatt gcgtaatatc gccatcatcg cgcacgtaga ccatggtaaa accaccctgg
    13441 tagacaagct gctccaacaa tccggtacgt tcgactctcg tgccgaaacc caagagcgcg
    13501 tgatggactc caacgatttg gagaaagagc gtgggattac catcctcgcg aaaaacaccg
    13561 ctatcaaatg gaatgattac cgtatcaaca tcgttgatac cccggggcac gccgacttcg
    13621 gtggtgaagt tgaacgtgta atgtccatgg tagactcagt gctgctggtg gttgacgcat
    13681 ttgacggccc gatgccgcaa acgcgcttcg taaccaaaaa agcgtttgct tacggcctga
    13741 agccgattgt tgttatcaac aaagttgacc gccctggcgc gcgtcctgat tgggttgtgg
    13801 atcaggtatt cgatctgttc gttaacctcg acgcgaccga cgagcagctg gacttcccga
    13861 tcgtttacgc ttctgcgctg aacggtatcg cgggtctgga ccacgaagat atggcggaag
    13921 acatgacccc gctgtaccag gcgattgttg accacgttcc tgcgccggac gttgaccttg
    13981 acggtccgtt ccagatgcag atttctcagc tcgattacaa cagctatgtt ggcgttatcg
    14041 gcattggccg catcaagcgc ggtaaagtga agccgaacca gcaggtcact atcatcgata
    14101 gcgaaggcaa aacccgcaac gcgaaagtcg gtaaagtgct gggccacctc ggtctggaac
    14161 gtatcgaaac cgatctggcg gaagctggcg atatcgtggc gatcacgggc cttggcgaac
    14221 tgaacatttc tgacaccgtt tgcgacacgc aaaacgttga agcgctgccg gcactctccg
    14281 ttgatgagcc gaccgtttct atgttcttct gcgttaacac ctcgccgttc tgcggtaaag
    14341 aaggtaagtt cgtaacgtct cgtcagatcc tggatcgtct gaacaaagaa ctggtacaca
    14401 acgttgcgct gcgcgtagaa gaaaccgaag acgccgatgc gttccgcgtt tctggtcgtg
    14461 gcgaactgca cctgtctgtt ctgatcgaaa acatgcgtcg tgaaggtttc gaactggcgg
    14521 tatcccgtcc aaaagtaatc ttccgtgaaa tcgacggtcg taaacaagag ccgtatgaaa
    14581 acgtgactct ggacgttgaa gaacagcatc agggttctgt aatgcaggcg ctgggcgaac
    14641 gtaaaggcga cctgaaaaac atgaatccag acggtaaagg ccgcgtacgt ctcgactacg
    14701 tgatcccaag ccgtggtctg attggcttcc gttctgagtt catgaccatg acttccggta
    14761 ctggtctgct gtactccacc ttcagccact acgacgacgt acgtccgggt gaagtgggtc
    14821 agcgtcagaa cggcgtactg atctctaacg gtcagggtaa agcggtcgcg ttcgcactgt
    14881 tcggtctgca ggaccgcggt aagctgttcc tcggtcacgg tgcagaagtt tacgaaggtc
    14941 agattatcgg tattcacagc cgctctaacg acctgactgt aaactgcctg accggtaaga
    15001 aactgaccaa catgcgtgct tccggtactg acgaagctgt tgttctggtt ccgcctatcc
    15061 gcatgactct ggaacaagct ctggagttca tcgatgatga cgaactggta gaagtgactc
    15121 cgacctctat ccgtattcgt aaacgtcacc tgacggaaaa cgatcgtcgt cgcgccaacc
    15181 gcgcaccgaa agacgattaa tttcgttttt tagttataaa aaacctgcca tcgctg
//
LOCUS       DAEEKQ010000093        15148 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.093, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000093 DAEEKQ010000000
VERSION     DAEEKQ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.093"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            88..444
                     /locus_tag="KLK57_002698"
     CDS             88..444
                     /locus_tag="KLK57_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBE0893027.1"
                     /translation="MQWYLSVLKNYVGFSGRARRKEYWMFTLINAIVGAIINVIQLIL
                     GLELPYLSMLYLLATFLPVLALAIRRLHDTDRSGAWALLFFVPFIGWLVLLVFFCTEG
                     TSGSNRYGNDPKFGSN"
     gene            complement(495..1391)
                     /gene="yhaJ"
                     /locus_tag="KLK57_002699"
                     /pseudo
     CDS             complement(495..1391)
                     /gene="yhaJ"
                     /locus_tag="KLK57_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
     gene            1496..2197
                     /locus_tag="KLK57_002700"
     CDS             1496..2197
                     /locus_tag="KLK57_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HBE0893028.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            2220..2384
                     /locus_tag="KLK57_002701"
     CDS             2220..2384
                     /locus_tag="KLK57_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893029.1"
                     /translation="MSKKSAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2518..3828)
                     /locus_tag="KLK57_002702"
     CDS             complement(2518..3828)
                     /locus_tag="KLK57_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HBE0893030.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVRIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3856..5187)
                     /locus_tag="KLK57_002703"
     CDS             complement(3856..5187)
                     /locus_tag="KLK57_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HBE0893031.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5462..6826)
                     /gene="tdcG"
                     /locus_tag="KLK57_002704"
     CDS             complement(5462..6826)
                     /gene="tdcG"
                     /locus_tag="KLK57_002704"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HBE0893032.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAAWMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6898..7287)
                     /locus_tag="KLK57_002705"
     CDS             complement(6898..7287)
                     /locus_tag="KLK57_002705"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HBE0893033.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQACLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(7301..9595)
                     /gene="tdcE"
                     /locus_tag="KLK57_002706"
     CDS             complement(7301..9595)
                     /gene="tdcE"
                     /locus_tag="KLK57_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HBE0893034.1"
                     /translation="MKIDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9629..10837)
                     /gene="tdcD"
                     /locus_tag="KLK57_002707"
     CDS             complement(9629..10837)
                     /gene="tdcD"
                     /locus_tag="KLK57_002707"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HBE0893035.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVVIDTEMNNRSNSFGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10863..12194)
                     /gene="tdcC"
                     /locus_tag="KLK57_002708"
     CDS             complement(10863..12194)
                     /gene="tdcC"
                     /locus_tag="KLK57_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HBE0893036.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLVLKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(12216..13205)
                     /gene="tdcB"
                     /locus_tag="KLK57_002709"
     CDS             complement(12216..13205)
                     /gene="tdcB"
                     /locus_tag="KLK57_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HBE0893037.1"
                     /translation="MHITYDLPIAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(13304..14242)
                     /gene="tdcA"
                     /locus_tag="KLK57_002710"
     CDS             complement(13304..14242)
                     /gene="tdcA"
                     /locus_tag="KLK57_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HBE0893038.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            14431..14775
                     /gene="tdcR"
                     /locus_tag="KLK57_002711"
     CDS             14431..14775
                     /gene="tdcR"
                     /locus_tag="KLK57_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HBE0893039.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
BASE COUNT         3983 a         3992 c         3491 g         3682 t
ORIGIN      
        1 tataacatcc gaaaaagaaa gatacgaata gcatttacac aatacccgga atagactttc
       61 gtcactttta attaaaggga tgtttttatg cagtggtatt tatccgtatt aaaaaattat
      121 gttggtttct ctggtcgcgc acgtcgtaaa gagtactgga tgtttactct gattaacgcc
      181 atcgtcggcg ctattatcaa tgtcattcaa ttgattttag gtctggagct tccatatctg
      241 tctatgcttt atttgctggc aaccttcctt cccgtccttg cgcttgctat ccgtcgctta
      301 cacgataccg accgttcagg tgcctgggcc ttgctctttt ttgttccgtt tattggctgg
      361 ctcgtgcttc tggttttttt ctgcacagaa ggtacttctg gcagcaatcg ttacggaaac
      421 gatccgaagt ttggttcaaa ttaatcttag aattggggcg atatttcgcc cctttttatt
      481 aacaataata aatattattt tccgctaaaa agtttgggaa tttcccgcag acaccaggat
      541 ttcgcttccc ccatactgtc acgacgccag gccataataa tatcgatctc actggtcgat
      601 tccgggctga cgacacgcaa ccgcccttcc gcaatatctt tttcgaccat cggatacggc
      661 atcgtcgcca cgccaagccc cgccagtaat gcctgacgtt tatcttcaat cgtgctcacc
      721 gttaagcgcg gctgtttgtc cagcagttgt acggtcaaca ccgggcgctc acgagcggta
      781 tccgctaccg caattccacg atatttcacg cgcgtcactt cagataacgg ttccggctcc
      841 tgatgaatcg ggtgatcagg cgcggcaacg tagacgttca ttaacgtata gagcttgcgc
      901 gagttgatct ccgacgagga acgaaaatgc atatccggcg cgataacaat atccgcccgc
      961 ccctgctcca gtcgttccta cgcccccgcc agcacttcgg tgatgattgc cagttgggta
     1021 ttggcttttg ccgccagttt gtcgattaac gggaaaaagg caggtgtcgg taccagcgct
     1081 tcggtcacaa tggtgagatg cgtttcccaa ccgcgcgcga gggcttccgc atcggtagtc
     1141 agtttatctg cggcttccag caaaacgcgc cctcgctcca gcaacatcct cccgacattg
     1201 gtgaatttgg tgcgatggcc cgagcggtca aacagcacca catccagctc ttcttccagt
     1261 ttttgcatgg tgtagctaag tgcggaaggc acgcgtccca gctcatccgc cgccgccgca
     1321 aaactgcccc ggcgatcgat cgcatccata acccgtagtg cttccagcgt taatgccctt
     1381 tctttggcca tttcgttctc attcaggaaa tttgaacata ccgggcagaa tatctggcta
     1441 acaatgtagc gtccagcccc ttaccataaa aggaagtaaa gagaggtcaa gaattatgat
     1501 tactacccga actgccaggc agtgtggaca agcagactac ggatggttgc aggcccggta
     1561 tactttttcc tttggacact acttcgaccc gaaattgtta ggctatgcct ccctgcgtgt
     1621 gcttaaccag gaagtgctgg ccccaggtgc cgcctttcag ccgcgaacct atcccaaagt
     1681 cgatatttta aatgtgattc tggatggaga agcagagtat cgcgacagcg aaggcaatca
     1741 tgttcaggcc agcgccggtg aggcgttgct gctctctacc cagccgggtg tcagctacag
     1801 cgaacacaat ctcagcaaag acaaaccgtt aacacgaatg cagctttggc tggacgcctg
     1861 cccgcagcga gagaatccgc tgattcaaaa actggcgctt aatatgggca agcagcaatt
     1921 aatcgcctcg ccagagggta cgatgggaag cctgcaatta cgccagcaag tgtggctgca
     1981 ccatatcgtg ctcgacaaag gcgaaagtgc gaatttccag ttgcatgggc cacgcgcgta
     2041 tttgcaatcg attcacggga aatttcatgc gcttacgcat catgaagaga aagcggcgct
     2101 gacctgcggt gatggggcgt ttattcgtga cgaggctaac attacgctgg ttgccgattc
     2161 cccactgcgc gctttgctga tagatttgcc tgtctagttg tttttacagg gagatgatga
     2221 tgagtaaaaa atcggccaaa aagcgccagc cagtgaagcc cgtggtggcg aaagaacctg
     2281 ctcgcaccgc caaaaatttt ggctatgaag agatgttgag cgagctggaa gccatcgtcg
     2341 cggatgctga aacgcgttta gccgaggatg aagctaccgc gtaaagacag gttgccggat
     2401 gcggcgaaaa cgccttatcc ggcctacaaa atccaacaac attaacgaat taaacaaccc
     2461 gtaggccgga taaggtgcgc cagcatcgca tccggcacaa tactcaaaac ctggcgttta
     2521 tctggccttg ctcgccataa tctcgataat ctgccgatcc gtttgctgca tcgaatggct
     2581 tgctaacgca cacaggttgg caatcgactg ctcaacatca tgcgccacga tcccttcatt
     2641 gccggtcacg gcggtatcat ccagcgccat taacaccgct ttccacgcag ccgaagcact
     2701 ggtcgaaacc ttcatcgcgc agctgttcga cgcaccatcg caaatcatgc cgctgacatc
     2761 gccgatcata ctgctgatcg ccatcgagat agtttcataa cgcccatcca ccagccatgc
     2821 catcccggcg gcggccccca ttgctgcggt cgttgcggca caaagtgcag acaaacgcgg
     2881 taactggtta tggatgtaaa ttgcgctcaa atgagaaagc atcagcgcac gcgccagtcg
     2941 ttcatcatcc gctccgaagt gttctgctac aaccaccaca ggcattgttg cggtgatccc
     3001 ctggttaccc gagccggagt tactcatagc cggaagcgta gcgccgccca tacgcgcatc
     3061 ggatgccgcg ctggtacgaa tcacaatgga tgaagagaga tctttcgcca gcaaaccgcg
     3121 ctcgcactgt ttttccagcg tcgcgccaat atgcagcccc cacttaccgc tcaaaccttc
     3181 ctgcgataac gcacaattta gcttcgcgga atcgagaata aagcggatcg ccgcaaacgg
     3241 gacttcattg acgaacttca ggatctcagc cagcgtcgtt ctggaaagca ccgtcagcgg
     3301 agactcttgc tcgccctctg ccacacacgc ctgctgggta aacaccacac cattgtgcgt
     3361 ctcaatacgc acaatgttgg tatgcccgcc gacgatggtg acacacgccc acttctcacc
     3421 gttccagact ttggcgcgtg agaagaggat ttcattgcaa ggttcctgga tcttaacgga
     3481 gactttcccc gccgccagca gtgctttggc atcggcaatt gcctgcgcag ttgcgtcttt
     3541 cagcacttcc agcccggcgt tggcatttcc acctaacgcc cccagcgccg ccgcaatcgg
     3601 cagccccacc attcccgtgc cgggaacggt gacgcccaga ccgttcttca tcagatttgg
     3661 cgaaacccag gcttctacac gttcaaccgg accttccagt tctgccgcag caaccgccgc
     3721 cgccagcgcc agtgaaatcg gttcagtaca tcccagcgcc ggttttactt cctcctgaac
     3781 ggcgaggatg taacgctgcc ataacggatt taaagtcgaa tcaaacatat taaaaacctt
     3841 aaaatttcag gtaaatcaag aaaatgccag gaacggagaa acacaaagca acaaaccagt
     3901 gacgataatc aggtacagag acatcccttt gtatttgtgc aatgccggta ctttgtaaac
     3961 cagccacgcc gggatcaggc accctaccat gccgaaaatc gggctacaga tagaggtgaa
     4021 gctcaacacc ggtgcgttca gtacgatagc gctccaggcc agcaaaatgg cgaaaatcat
     4081 gatgccgcgc tgaacaagat tttcgttaat cttctcggca ggcatcttgc gacgcaggat
     4141 gttcattacg atcccttgcg ttgcttcgcg aaagcctaaa tagacgccaa agaatgcagt
     4201 catgacagca aagatattga gaatgacgct gaccactttc acccacgctg cgccgtcacc
     4261 gctaataaac tgtgcggcaa tcgccagcgc ggaaatattc tgctcgtagg cttttaccgc
     4321 ttcgtcatgc cccatcgcca gcgtgaacga cacggcgtag aaaaagacgg tgacaaacaa
     4381 aatgccaaac gcgatattca ttgcccgcaa tgctttatgc cgcgccactt caatagattt
     4441 ttcccgcgag cgataagaga tcaccatcgg gcttaacgtc tggataaaca aaatcgacgt
     4501 cagggtaaac ggcagcgtaa taatggcgtt tttcaccagc agccccagcg gcggtagcga
     4561 accgacgtta tacagatgcc acattcccac catcgacacg cccagcgccg cgaccaccag
     4621 cagcttggtc agcaccatgc cggtcgaaat tttgaataac aatttctcgc cgcgtgaaga
     4681 gatcgccacc agaatgcaaa tcagtaccag accataaaag ggactgtctg acagcaaccc
     4741 ttccgtcacg ccgaaggtat gcagatagga agcactatcg ttggtgatgg cggtggaata
     4801 gacgaacatc caaatcacca gcattacgaa atagagcgca cctaacagga tgccccagtt
     4861 tttacctaaa taaccgctaa tgacactcgg gtaatcttta cattctggtg attctgccag
     4921 cgtattaata aacaaccgct gaaacagata cattgccggg taaccaatca ccgatgagag
     4981 caaaaatacc cacaatccca tcaaaccgac ctgcaccggg agaaaaacaa tccccgcgcc
     5041 aatcgccatc ccgatactca taatcaccca gccggtgtca gtactgtcga atttgatcgc
     5101 ttctcgccac tcgctctcac tcattccagc acgacccgcc ggggtcgaag cgtctgcaat
     5161 gacgccttta ttcgatgcga tttccataat ttctcgctca atattttgta gggcttatta
     5221 tttttttccg agccgcatca aggcgatatg cggtctacgt ttttgcaggc gagttattag
     5281 aataaagaaa tgatacgcgc gaggtcagag aaaatcttca caatcaagac tttgaaataa
     5341 cggagatgga taagaatttt ctactaaatt aatcgcaaga gaatattcat tctctatttg
     5401 cggcagatca caaaaaaaag gtgcacattt gtgcacccaa ggatgaaagc tgacagcaat
     5461 gtcagccgca gaccacttta atggccagtc ctccgcgtga tgtttcgcgg tatttatcgt
     5521 tcatatcttt gccggtttca tacatcgtct cgatcacttt atcgagtgaa acacgcggtg
     5581 ccgaggtgcg gcgcatcgcc atccacgcgg cgtttactgc tttcacggca ttaatggcat
     5641 tacgttcaat gcacgggatt tgtacctgtc cggcaaccgg atcgcaggtc agcccaaggt
     5701 tatgctccat cgcgatttcc gccgcattgc atacctgcgc cggactgccg cccagtagtt
     5761 cagttaaccc tgccgccgcc attgaacagg ccacgccaat ctccccctga cagccgacct
     5821 ccgcgccaga gatggaggcg ttcattttat acagcgcgcc aatagccccc gctgccagaa
     5881 aatagcgggc aattgaccgc tcgtttaccg gacgacggaa cttatcgtaa taagccagca
     5941 ctgccggaat aatgccgcac gcaccgttag tcggtgccgt taccacgcgc ccgccagctg
     6001 cgttttcttc actaaccgcc agcgcgtaca tgttgatcca gtcgatgaca ttcatcggat
     6061 cgttagagat gttatcgcta gaaaccagct gacgacgcag cgctacggca cgacgcggca
     6121 cattgagcgg accaggcagc acgccttcag tgttcatccc gcgttcaata ccgtcatgca
     6181 tcacttgcca gatacgggca aatcctgcgt caatttccgc tttgctgcgc agcgccagct
     6241 cgttgtgcat catcaggcca gatatagaca ggccgttgta atcacacatt ttcagcagtt
     6301 cacctgctga gtggaaatcg taaggtacgg acgtttcgac atcgtgcgac aggccgaagt
     6361 gttcttcttc gacaataaac ccgccgccga cagagtaata ggttttactt aatagcgctt
     6421 cctgtccctt ccaggcagtg atccgcattc cgttctcatg gcgaggcaac atttcgggat
     6481 ggaagataat gttctttgct acaggaaaat caacaatatg cgcaccagat gccactggca
     6541 gccgcccgct gcgcgttact aactctataa atgcagggat ctcatcaatg acaacatcct
     6601 gcggactgtt tcctgccagt cccatgataa tggcgacatc cgtggcatgg cctttgcccg
     6661 tcagtgacaa cgacccatac agatcgacca caatatggct cgtcgcggtt aataagccgc
     6721 tactttccag ccgatcaata aaactttttc cggcattcat tggccccacg gtatgcgaac
     6781 tggagggacc aatcccaatt ttgaaaatat cgaatgcact aatcatatcc acaccctcgg
     6841 attgccgttc agtgaagtgg agcggaacga ccttgcgacc gtcccgctca cgaggcttta
     6901 cgcactacgt actgcgatag cttcaatttc cagcttcaca tctttcggca aacgcgcaac
     6961 ctgcacacag ctccgggtcg ggtaggtcgc ctgatgctca tcgaagaact gcttatagac
     7021 ttcattgatg gtggcaaaat cattcagatc ggtgatgaac acggtcatct tgatgatatc
     7081 gcccacgctc agcccggcag caaccacgat cgctttgacg ttttcgaggc ttaaacacgc
     7141 ctgatcttgc acatcagccg ggatctcacc ggtctgcggg caaaccggaa tttgcccgga
     7201 ggtgaagacc atgctgccta aatcaacgcc ctgaacataa gggccgattg cgcctggggc
     7261 acgttgcgtt tcgataatct ttttcataca tcctccggcg tcagagcgcc tgggtaaaag
     7321 tacgtgaaat aacatcctgt tgctgttcac gggtcagtgc gttgaagcgc acggcgtagc
     7381 cagagacacg gattgtcagg ttaggatatt tttccgggtg ctcgatggca tccagcagca
     7441 tttcccgatt cattacgttg acgttgaggt gttgaccgcc ttcgacatcc gcttcgtggt
     7501 ggaaataccc atccagcagg ccgacaaggt tggttttacg tactggatct tctttgccca
     7561 gcgccgcagg aacgattgag aaggtgtacg agatcccatc tttggcgtag gtgaacggca
     7621 gtttcgccac cgacgtcaat gaggccacgg cacctttgcg gtcacgacca tgcatcgggt
     7681 tagcgcccgg cgcgaacggt gttccggcgc gacgaccgtc cggcgtatta ccggttttct
     7741 ggccgtacac cacgttagaa gtgatagtca gaatcgactg ggtagggacg gcgttgcgat
     7801 aggttggcag cgctttaatt ttcttcataa agcgttcaac caggtcgcag gcaatgctgt
     7861 ctacgcgctc gtcgttgttg ccgtactgcg gatattcacc gtcgatttca aagtccaccg
     7921 ccaggccgtt ttcgtcacgg attggtttca cgcgggcata tttgatggca gacagggagt
     7981 ccgtcgccac cgacaggccc gcgatgccgc atgccatagt gcgatagaca tcacgatcgt
     8041 gcagcgccat cagcgaagct tcgtagctgt acttgtcgtg catgtagtga atgatattca
     8101 gcgcgctgat gtactgcacc gccagccagt ccatgaagtg atcgaggctg tccatcactt
     8161 tgtcgtaatc cagcacgtcg tccatcagcg gtgctgtttt cggcccgacc tgaatcttca
     8221 gcttctcgtc caccccgccg ttaattgcgt acagcagtgt tttcgccagg ttagcgcgtg
     8281 caccaaagaa ctgcatttgc ttaccaatca ccattgggct gacgcaacaa gcaattgcgt
     8341 aatcgtcgct gttgaagtca gtacgcatca gatcgtcgtt ttcatactgc aaggaagagg
     8401 tgacgatcga cacctgcgcg gcatattttt tgaaggcaat cggtaattct tccgaccaaa
     8461 gaatggtcag gttaggttcc ggtgccggcc ccatagtgtg cagggtgtgc aaatagcgga
     8521 aggagttttt ggtcaccagc gtacgaccgt ccagccccat cccgccgatc acttccgtcg
     8581 cccagattgg gtcgccggag aacagcgaat caaattccgg cgtacgcaag aagcgcacca
     8641 tacggatctt cataatgaag tgatcgatca gttcctgtgc ctgctgctca ttgagtacgc
     8701 ctgctttaaa gtcgcgctca atgtagatat cgaggaacga cgccgtgcgc cccagcgaca
     8761 tcgcgccgcc gttctgtgat ttcactgccg ccagataagc gaagtagagc cactgcaccg
     8821 cttcctgcgc attctgcgcc gggcgagaga tatcaaagcc atatttcgcc gccatttcct
     8881 gaatctgcaa cagcgcatga cgatgctctg ccagctcctc acgcagacgg atggtggctt
     8941 ccagatcttc gcctttttcc agacgggact ggagatcggc aaattgcagt tcgcgttcac
     9001 gcaccagata actgatgcca tacagcgcta cgcggcgata gtcaccgata atgcgcccac
     9061 ggccatagcc atctggtaaa ccggtcagca cgccagattt acggcagcgc agcatatccg
     9121 gtgagtaaac atcaaatacg ccctggttat gggttttacg cagatcggta aacagatatt
     9181 caaattcact gtccatttca cggccatagg cgtggaatga acttttaatc atattaatgc
     9241 caccgaacgg atgcagcgcg cgctttaatg gcgcatccgt ttgcagacca acaatttttt
     9301 ccagcggctg gttaatatat cccgcatcat gagcggtaat tgtggtggca atattggtat
     9361 cgaaatcaac cggcgcgtgg gttgcgtttt cgatacggat gccttccatt actttttccc
     9421 acaattccgt ggtggcaggc gtcgcttcgg cgaggaaaga ttcatcgcct tcatacggtg
     9481 tatagttatg ttgaataaaa tcgcggacat taatttcgtt tttccagtcc gtacctttaa
     9541 agccaagcca tgcgtcggcg tacagcttat cgctggtatc aatatctatc ttcatgaaaa
     9601 ataatctctc tacaatactt caactaaatt atgcaaattc tgcgggcgcg ttaactttgc
     9661 ctaaatgaat ggcatccaaa gcaatcattt tttcttcgtt agtcggaata acggcacaaa
     9721 tgacacgcgc attttcactg gaaacaattc gctctccaaa agagttagag cgattattca
     9781 tttctgtatc aatcactacg cctaatacag ccaaatgttc catgaccaga cgacgaatta
     9841 aacttgaatt ctctcctatt ccgccggtga atataattcc atccaggcga cgtaatgaag
     9901 ctgcgtgtcc ggcaatatga cgggcaattc ggtgaacaaa ggttttaatt gccagttgcg
     9961 cgcgttcgtg accttcatgc caggcttttt ccagaacacg taaatccgaa gaaagaccgg
    10021 aaatacctaa taatcccgac tctttattca ctacgcgttc caggtcaccc aggctctggt
    10081 tggtttggct ggcgacccag gacatcgcac caaagtcgac atcgccactg cgggttccca
    10141 tcatcaagcc ttccagcggc gtcattccca ttgaagtatc aacactctga ccgttgcgaa
    10201 ccgcgcagat tgacgcgcca ttgccaagat gcgccacaac caggccggag tcatcttccg
    10261 ccagattcag cagagaatgt gcgcgctggg aaacatagcg gtgcgacgtg ccgtggaaac
    10321 cataacggcg tacacctaac tcttcataat atttccacgg caggccgtat aaataagctt
    10381 ccggagccat cgtctggtgg aaactggtat caaataccgc cacctgagtt acgcccggaa
    10441 ataattgctg cgccgattca ataccactta aattggcgta attatgcagg ggtgccagtg
    10501 gagaaacgcg acggatatta tcaatgactt catcggtaat aatggcggac tcggtaaaaa
    10561 tactgccgcc gtgagcgatg cggtggccaa ttaaggccac actgtcattt aaattccgtt
    10621 tttccagttc aaatgcaatt gccttcaatg caccttcgta gctgtggtga gccagcggtg
    10681 ctggctctcc cccatttacg gataagaatg cattttccga gttaataccg tcggcaatac
    10741 ctgacattaa tacttcacag tcgctggcat cgagcacgga aaacttaatc gaagacgaac
    10801 cacagttaat aaccaaaaca accggaaatt cattcatctc ttttctcatc ctgagttacg
    10861 gattaaaaca gtttgtatac gatgttcagg atggtcagca gaccaatcac ggtaacaaac
    10921 acgttatcca gacgaccacg gtatttcgcc agagacggcg ctttacggat ggcatacatc
    10981 ggcaacaggc acagcaggga tgcgataatc ggtgcgccca tggcttcaat caggtcgagg
    11041 atgttcgggt tggcgtaggc aacaacccag gtggagccca tgatgaagat catgctgata
    11101 gtgttcagtt tacccagcga cactttagtt ttgtcgcctt tataaccaaa cttcaggacc
    11161 aggccattca gaccttccag cgttcccaga tagtgaccga agaaagattt gaagatagcc
    11221 acgagtgcga tgatggaagc cgcatattcc agtgtaatcg cgaacgttgt tttggtaccg
    11281 gtcatggacg caaagtggtt agccagataa gaaagcactg gaatattctg cgctttggct
    11341 tccgccatgt tggccggaga cagcgtaaat aggcagctaa aggcaaagaa catcaccact
    11401 gcaaccatca gcatgctggc acgagaaatg atttgggaac atttacgttc ggtgaagtcg
    11461 cgaccgaagt ctttctcata ctcttcacgt ttagaaacca cgaaggaaga gacgattggc
    11521 gagaaattaa aggagaaaac catgatggaa atccccagcc agacggtgat caggataccg
    11581 tcatgaccgg ttaacgacag cgaaccgagg tcaacctggt cgataactgc agagttccag
    11641 taagggatca gcgacagaga aatcagcacc aggctggcga taaacggcca taccaggtag
    11701 ctcatcactt taaccatcag atccttacca aaccagatga cgaaagccat cagcagcagc
    11761 aggaacagcg ccacaaagcc gcgattcagc ggtgcaaagc cgagctggtt ttcccagaac
    11821 gtcataaagg tattggtaat agtaacgcca taaatccaca gcagtgggca aatcgcgaag
    11881 aagtacagga acgtgataac cacgccgcca gttttaccaa aatgctcttc caccgtttcc
    11941 gtaatgttgc cggaagggtt agagccagaa agacatagac gcgccagcgc ccggtggcaa
    12001 taaaatgcga tggggtatgc caataccaac atcagaagaa tcgggatcag tccgccaaaa
    12061 cctgcgcgga tagggaagaa cagcaccccg gcgccgattg ccgtaccaaa caagcctaac
    12121 gtccatgtgg tatctgattt acgccaggac gattgttttg tctggctgga tacaatgcta
    12181 tctgaagtac tcatatccta tcctcaacga attaattaag cgtcaacgaa accggtgatt
    12241 tgagagacgc gagaaagatc gatattgccg ccggaaataa tactgacggt ttttctgttt
    12301 tgaatatatt ggtctaattt accgcttaat aatgcagcac atgccagagc gcctgcgcct
    12361 tcggtgacga ctttattgcg ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg
    12421 ctgaccagca cgatgtcatc gactaattca cgaacgattt cgtaagttaa attacccggg
    12481 cgggagacat cacaaccatc cgccagggtg ccggtagttc ggtgcgtggt tatttctccg
    12541 gagtggaaag aagccgccat gccgtgaacg ttttcagact gtacgccaat aacacgaatg
    12601 gtcgggttaa tagatttaat tgccaccgca ataccagcaa ttaaaccgcc accaccaatt
    12661 ggcacaatca cgttatcgac atcatagaga tcttccataa tttccagacc aatcgttccc
    12721 tggccagcaa tcactttcgg atcatcgtaa ggtgggataa aaatacggcc ttccatttcg
    12781 acaatttcgc tcactttagc gatagtgtcg ttgaagttat caccatgcag aacgacttct
    12841 gcggagtagt cgcacgttgc cgctactttg gattttggcg caccttttgg catcaccact
    12901 ttaccgtcga tacccagcat cgcgcaggag agcgaaaccc cttgcgcatg gttgcccgca
    12961 gaacaggcca ccacgccttt gcgtttttcc gcatcggtca gtgaacttaa tttattaaat
    13021 gcgccacgaa ttttaaatga acccgtacgc tgcatatttt caaacttcag gaatatttca
    13081 cctttgcaac gttcactaaa atagttggag cgaggcatgc ctgttttata aattcgccca
    13141 gccagtcgtt gtttcgcttc aataatatca tcaatagcaa tcggcagatc gtatgtaata
    13201 tgcattataa aacctcttcg cctgaattaa atatgtaggt aaccgtaacc gacacctgca
    13261 aaacagacag gtggattatt tatgtttaaa taaaaacaaa taactaacca acttctatta
    13321 attgccttcg tctacaccca ttataagatg aatactcttt ggctaattcc accaaaaccg
    13381 atgctgcttt tttaatacga taatttttcg accataccgc ggcatattgt gccacaggta
    13441 atgtttcttc aaccggaata gtaataaatt gattagaacc aaaaggtgac gtcatatcac
    13501 aaggaattac agttaagaaa tcagcattga gaacaagatt ataaattgtc acgactgagt
    13561 cggttttaac gatgttttca atactgatgc catttctttg taacgtagta agcagttcgc
    13621 tgtagtaccc catattagtt tgtggcaaca cccactgttc gttcttcaac gactccagcg
    13681 tggtggtgcc ggtgcatgtt cgggacttac tggctaccag cacaaactcg gactcgaaca
    13741 gcggctcaac atgtaaatcc tgaagcttca tttctgcact taatgtacca atcgcaaaat
    13801 ccagtcgacc gtcgcggatt gccggtaaga acgaagacag ttgcgcttca tacatagaaa
    13861 cctgcgcttt cgggaacacc tctttgaact tgttgatcat ccctgacata aaagtaaaac
    13921 caatcaatga aggaaaacca aatgagactt ccaccaccgc ctcagaagac ataccgctta
    13981 tctcattaac catatttttc atttcacggg taatggattc ggaacgggag agtaacaatt
    14041 gaccggcagg tgttaatgtt acaccggtat ttttccgcac cactaattcc acaccaaaat
    14101 aatcttcaat atcgttaatg attttactga cggccggttg agttaaccct aattcttttg
    14161 cagccgagcc gatagagcca cttctaatga cttcctgaaa gactaccagg tgctgcgttt
    14221 tcggaagaag aatagtgctc ataacgacct acgttaatta cctcattgac ggcatgaagt
    14281 gtatcaaaat gaaatgaaca ggatatgtgc gaccactcac aaattaactt tcaatacttt
    14341 ccagggtatc gtcattacaa ttaaatacct ttaaatatca acaagttaaa gcataaaaat
    14401 cagcataaac cctgattttt gtcaaaaaaa atgacgggga taaccatatt ttatggcgat
    14461 aacatcattc gttatgtggt taatacaaaa aaggggctga gaccatattt caagcaatta
    14521 ccggataatt accaggcaaa atttgagtta aatttaatga gcaagttctc aaattttatt
    14581 ataaataaac cattttcagc gataaatact gcggcacgtc acattttttc acgctatttg
    14641 ttggagaaca aacatttatt ttatcaatat tttaaaattt cgaatacagg tattgatcat
    14701 ctcgaacaat tgattaacgt caactttttc tcttctgaca ggacgtcatt ttgtgaatgc
    14761 aatcgttttc cataaattct tctcccctca taggcgacga atagcatttt gtgttgagga
    14821 tcacaaaacg aataattgct gatcgccgcg ataaggtcag acaaagacaa caagggaaat
    14881 tttcacagag cttttgatcg tcgtaggcca cagaatgttg cttcgtttac tgtaacgccg
    14941 ggtaaatgag cgttttttga tagtgcgaaa gaaccctgcg aggaaaataa gcatctatta
    15001 ttgtttgtca tgacgacaag gcaggcatca atgaaaggct tcccaattgc acatattttt
    15061 cacccttcaa tcccgccaat gcacgcagtg gttaacaatc acaatagaaa tattgattat
    15121 tggacggtaa aaagaaagtt tgcagaaa
//
LOCUS       DAEEKQ010000094        15084 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.094, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000094 DAEEKQ010000000
VERSION     DAEEKQ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15084
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.094"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..781
                     /locus_tag="KLK57_002712"
     CDS             <1..781
                     /locus_tag="KLK57_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893040.1"
                     /translation="NKESLFFMSVMELRDLLIALESRLHQKHKLNAAARLQYEQARNK
                     VIKKMAENIPEILVDELKNADINRRVNTLELTDNQGENLTFVLTLHDGSKCELVVNEL
                     QIEMLARAIIHAINNAEMRELALRITSLLDFLPLYDVDCQDNGNLEYDTYSQPEWKHN
                     LFDHYLAVLYRFKDESGKEQFSGTVVKTREATPGKEIEAITRRMLDFSPRLKKLAGVP
                     CQVYVRTVAANNAQPLTQDQCLRALHHLRVQSTSKTAPQAK"
     gene            complement(984..1337)
                     /locus_tag="KLK57_002713"
     CDS             complement(984..1337)
                     /locus_tag="KLK57_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HBE0893041.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(1475..3121)
                     /gene="groL"
                     /locus_tag="KLK57_002714"
     CDS             complement(1475..3121)
                     /gene="groL"
                     /locus_tag="KLK57_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HBE0893042.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(3165..3458)
                     /locus_tag="KLK57_002715"
     CDS             complement(3165..3458)
                     /locus_tag="KLK57_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HBE0893043.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            3734..4990
                     /gene="yjeH"
                     /locus_tag="KLK57_002716"
     CDS             3734..4990
                     /gene="yjeH"
                     /locus_tag="KLK57_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HBE0893044.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAAQVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LTCFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLFLPKRKTPENGITT"
     gene            complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="KLK57_002717"
     CDS             complement(5012..5482)
                     /gene="fxsA"
                     /locus_tag="KLK57_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HBE0893045.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDR"
     gene            5819..7255
                     /gene="aspA"
                     /locus_tag="KLK57_002718"
     CDS             5819..7255
                     /gene="aspA"
                     /locus_tag="KLK57_002718"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HBE0893046.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            7373..8674
                     /gene="dcuA"
                     /locus_tag="KLK57_002719"
     CDS             7373..8674
                     /gene="dcuA"
                     /locus_tag="KLK57_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HBE0893047.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            8790..9128
                     /gene="cutA"
                     /locus_tag="KLK57_002720"
     CDS             8790..9128
                     /gene="cutA"
                     /locus_tag="KLK57_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HBE0893048.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            9104..10801
                     /gene="dsbD"
                     /locus_tag="KLK57_002721"
     CDS             9104..10801
                     /gene="dsbD"
                     /locus_tag="KLK57_002721"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HBE0893049.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSHFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVPQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTHLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGMLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPIFLLERVIGDIWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKKFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            10838..11413
                     /locus_tag="KLK57_002722"
     CDS             10838..11413
                     /locus_tag="KLK57_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0893050.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            11520..11595
                     /locus_tag="KLK57_002723"
     tRNA            11520..11595
                     /locus_tag="KLK57_002723"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11553..11555,aa:Phe,seq:gaa)
     gene            12212..13749
                     /gene="cadC"
                     /locus_tag="KLK57_002724"
                     /pseudo
     CDS             12212..13749
                     /gene="cadC"
                     /locus_tag="KLK57_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
     gene            14114..>15084
                     /gene="cadB"
                     /locus_tag="KLK57_002725"
     CDS             14114..>15084
                     /gene="cadB"
                     /locus_tag="KLK57_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HBE0893051.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLYLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLL"
BASE COUNT         3708 a         3764 c         3616 g         3996 t
ORIGIN      
        1 caataaagaa tcgttgttct ttatgtctgt catggaactg cgcgacctgc tgattgcgct
       61 ggaaagtcgt ctgcatcaaa agcataaact gaatgctgct gcacgccttc agtatgagca
      121 agctcgcaat aaagtcatta agaaaatggc agaaaacatc ccagaaattc tggttgatga
      181 gcttaaaaat gccgatatta atcgtcgggt taacacgctg gaacttaccg ataatcaagg
      241 cgaaaatctt accttcgtat taacgctaca tgacggtagc aaatgtgagt tagtcgtcaa
      301 tgagttgcag attgaaatgc tggcgcgagc aatcattcat gcaattaata atgcggaaat
      361 gcgtgaactg gcattacgta ttacctcact gttagatttc ctgccgttat acgacgttga
      421 ctgccaggac aatggcaatc tggaatatga cacctattcc caaccggaat ggaaacataa
      481 cctgttcgac cattatctgg cggtgcttta ccgctttaaa gatgaaagtg gcaaagaaca
      541 atttagcggc acagtggtga aaacgcgtga agcaacgccg ggtaaagaaa tagaggcgat
      601 cactcgccga atgcttgatt tcagcccacg actgaaaaaa ctcgcgggtg tgccatgtca
      661 ggtctatgtt cgcaccgtag cggcgaataa cgcccaaccg ctaacccagg atcaatgctt
      721 acgcgcgctg catcacttac gtgttcaatc caccagcaaa actgcaccac aggcgaaata
      781 atctcatctt tgctaatgcg aggctggagc catccagcct gaaatgactg ataacgtgat
      841 cgttggtcat gccggatgcg acgtaaacgc cttatccggc ctacaaattc tcaagcattc
      901 aatcagttgc aatgcctgct gtaggcctga taagcgaagc gcatcaggca ataatacgtt
      961 tgtcacccag cctctggatc acatcagttc gggcacttat aaacctgacc gataatctgg
     1021 ctatccgtcg ggacaaaact ggacaacatt ccctgcgacg ggctgctgat gccataaatc
     1081 acgttaccgc ccattgcagc cgcctggttg cgcagatcgt ttgctgcgcc gcgcatagaa
     1141 ccgccctctt ctccgtgttg cccggaaagc cagttgcttt gcttacctgt cgcagtacca
     1201 atcagctggc actctgcgcc tggctgctcg tccacaatgc gtacactctg accggcagca
     1261 ctcaattcgt tgctggagct acaacccgcc atcagtagcg cggcaccgac aatccctgct
     1321 aagtatttta cgtgcatgtt attccccata atcaatgagc tggacgccac tcgcgtcgtc
     1381 cgtgtctgaa tcttatacta gaaagatgac caaaagaaaa acccccagac atttctgccc
     1441 gggggtttgt ttatttctgc gaggtgcagg gcaattacat catgccgccc atgccaccca
     1501 tgccgcccat accgccagca gcgcctaagt cagctgcatc atttttcggc aggtcggtaa
     1561 ccatgcactc ggtggtgatc atcaggccag ccacagaagc cgcgtactgc agagcagaac
     1621 gggttacttt ggttgggtcc aggataccca tgtcgatcat gttgccgtat tcttcggttg
     1681 ctgcgttgta accgtagttg ccgtcgccgc ctttaacggt gttagcaaca acagacggtt
     1741 cttcgccgca gttcaggacg atctgacgca gcggagcttc cattgcacgc agtgcaactt
     1801 tgatacccac gttctggtct tcgttctgac cacgcaggtc agccagttta gacgctacgc
     1861 ggatcagcgc aacaccacca ccagcaacca cgccttcttc taccgcagca cgggtcgcgt
     1921 gcagggcgtc ttcaacgcgt gcttttttct ctttcatttc aacttcggta gcagcaccta
     1981 ctttgataac tgcaacgccg cctgccagtt tcgctacgcg ttcctgcagt ttttcacggt
     2041 cgtagtcaga agttgcttct tcaatctgct gacggatctg agcaacacgg ccctggattg
     2101 cagcttcttc acccacgcca tcgatgatag tggtggtgtc tttgttgatc acaacacgtt
     2161 tagcctgacc caggtcttcc agggttgctt tttccagttc cataccgatc tcttcagaga
     2221 tcacggtacc gccagtcagg gttgcgatat cctgcagcat agctttacga cgatcgccga
     2281 agcccggagc tttaactgca gcaactttca cgatgccacg catggtgtta acaaccagag
     2341 ttgccagcgc ttcgccttct acatcttcag cgatgatcag cagcggtttg cctgctttgg
     2401 caacggcttc cagaaccggc agcatttcgc ggatgttgga gattttcttg tcagccagca
     2461 ggatgaacgg gctttccagt tctactgcgc cagtttccgg cttgttgatg aagtaaggag
     2521 acaggtagcc acggtcgaac tgcatacctt caaccacgtc cagttcgtcc tgcagaccgg
     2581 taccgtcttc aacggtgata acgccttctt taccgacttt gtccatcgct tcagcgatca
     2641 gtttacctac ggtttcgtcg gagttagcgg agatggtacc aacctgagca atcgctttag
     2701 agtcagagca cggtacggac agcgctttca gttcttcaac tgcagcggta acggctttgt
     2761 cgataccacg tttcaggtcc atcgggttca tgcccgcagc aacagctttc agaccctcag
     2821 tgatgatagc ctgagccagt acagttgcag tggtggtacc gtcgcctgca gcgtcgttcg
     2881 ctttagaggc aacttctttc accatctgcg cacccatgtt ttcgaacttg tcttccagtt
     2941 cgatttcacg agcaacggaa acaccatctt tggtgatggt cggtgcaccg aaagatttat
     3001 ccagaactac gttacggcct ttcggaccga gggtaacttt cactgcatct gccagtacgt
     3061 ttacgccgcg cagcattttc acacgagcgt cgttaccgaa ttttacgtct ttagctgcca
     3121 ttatctttat tccttaaatt cgtatgttca gtgtcgtgcg aggattacgc ttcaacaatt
     3181 gccagaatgt cgctttcgga catgatcaac acttcttcat tgtcgatctt ctcagatttc
     3241 acaccgtagc catcgttgaa aataacgatg tcgccaactt tcacatccag cggcttcact
     3301 tcgccatttt caaggatacg gccattgccg acagccagca cttcgccgcg ggtggattta
     3361 gccgctgcag agccggtcag aacgatgccg ccagcagatt tagtttcaac ttctttacgc
     3421 ttgacgatca cgcgatcatg caatggacga atattcattg ataactctcc tttgagaaag
     3481 tccgtatctg ttatgggtga cgccggagct tacgtggttt cccggctggt gaccagagaa
     3541 atggggatga ggattcgccc cttcaagggg gaaaaagaaa aaaaattctg atcagcacaa
     3601 aatcgggtga aaaccctgat tcacctcaca tttcatcgca atttcttcat cgccgtcata
     3661 agcgaatctg attgtgctac catcgaaaat ctacgcagtt gcccaaaatt tgggcgcaat
     3721 cggatatcaa cccatgagtg gactcaaaca agaactgggg ctggcccagg gcattggcct
     3781 gctatcgacg tcattattag gcaccggcgt gtttgccgtt cctgcgttag ctgcacaggt
     3841 agcaggcaat aacagcctgt gggcgtggcc cgttttgatt atcttagtgt tcccgattgc
     3901 gattgtgttt gcgattctgg gtcgccacta tcccagcgca ggcggtgtcg cgcacttcgt
     3961 cggtatggcg tttggttcgc ggcttgagcg agtcaccggc tggttgtttt tatcggtcat
     4021 tcccgtgggt ttgcctgccg cgctacaaat tgccgccggg ttcggccagg caatgtttgg
     4081 ctggcatagc tggcaactgt tgttggcaga actcggtacg ctggcgctgg tgtggtatat
     4141 cggtactcgc ggtgccagtt ccagtgctaa tctacaaacc gttattgccg gacttatcgt
     4201 cgcgctgatt gtcgctatct ggtgggcggg cgatatcaaa cctgcgaata tccccttccc
     4261 ggcacctggt aatatcgaac ttaccgggtt atttgctgcg ttatcagtga tgttctggtg
     4321 ttttgtcggt ctggaagcat ttgcccatct tgcctcggaa tttaaaaatc cagagcgtga
     4381 ttttcctcgc gcattgatga ttggtctgct gctggcagga ttagtctact ggggctgtac
     4441 ggtagtcgtc ttacacttcg acgcctatgg tgaacaaatg gccgcggcag catcgcttcc
     4501 aaaaattgta gtgcaactgt tcggtgtagg agcgttatgg attgcctgcg taattggcta
     4561 tctgacctgc tttgccagtc tcaacattta tatacagagc ttcgcccgcc tggtctggtc
     4621 gcaggcgcaa cataatcctg accactacct ggcacgcctc tcttctcgcc atatcccgaa
     4681 taatgccctc aatgcggtgc tcggctgctg cgtggtgagc actttggtga ttcatgcttt
     4741 agagatcaat ctggacgctc ttattattta tgccaatggc atctttatta tgatttatct
     4801 gttatgcatg ctggcaggct gtaaattatt gcaaggacgt tatcgactac tggcggtggt
     4861 tggcgggctg ttatgcgttc tgttactggc aatggtcggc tggaaaagtc tctatgcgct
     4921 gattatgctg gcggggttat ggctgtttct gccaaaacga aaaacgccgg aaaatggcat
     4981 aaccacataa tccggcgttt cgacattaat cctagcgatc gtctttatga tcaaggcggt
     5041 cgcggtcatc atcctttcgc tggtactcac catcaaaagt attaccgcca ccggtcccgg
     5101 cgctaaaacc gccgcctggc atgcgagaaa agcgcaaatg tggcatcaac ttcactgtca
     5161 gatgcttttg caccggcggc aataaaagca gaagaccgag gaagtcggtg aaaaagcccg
     5221 gtaataaaag cagcaaaccg gcaataatca gcgaaacact tttaatcatc tccgccgcag
     5281 ggttttcacc cgccgccatt ttttgctgca tcagcacgaa attcttaaag ccctggttac
     5341 gtaccagtga cataccgata actgacgtga atataaccag cacgagggtc agcaataccc
     5401 ccaatacatg ggcaacctga ataaagattg aaatctcaat atagacataa aggaaaatgg
     5461 caataaaagg taaccagcgc aaaggtttct cctgtaatag cagccggtta aacccggcta
     5521 cctgaatggg ttgcgaatcg cgttcagctt atattgtggt cattagcaaa atttcaagat
     5581 gtttgcgcaa ctatttttgg tagtaatccc aaagcggtga tctatttcac aaattaataa
     5641 ttaaggggta aaaaccgaca cttaaagtga tccagattac ggtagaaatc ctcaagcagc
     5701 atatgatctc gggtattcgg tcgatgcagg ggataatcgt cggtcgaaaa acattcgaaa
     5761 ccacatatat tctgtgtgtt taaagcaaat cattggcagc ttgaaaaaga aggttcacat
     5821 gtcaaacaac attcgtatcg aagaagatct gttgggtacc agggaagttc cagctgatgc
     5881 ctactatggt gttcacactc tgagagcgat tgaaaacttc tatatcagca acaacaaaat
     5941 cagtgatatt cctgaatttg ttcgcggtat ggtaatggtt aaaaaagccg cagctatggc
     6001 aaacaaagag ctgcaaacca ttcctaaaag tgtagcgaat gccatcattg ccgcatgtga
     6061 tgaagtcctg aacaacggaa aatgcatgga tcagttcccg gtagacgtct accagggcgg
     6121 cgcaggtact tccgtaaaca tgaacaccaa cgaagtgctg gccaatatcg gtctggaact
     6181 gatgggtcac cagaaaggtg aatatcagta cctgaacccg aacgaccatg ttaacaaatg
     6241 tcagtccact aacgacgcct acccgaccgg tttccgtatc gcagtttact cttccctgat
     6301 taagctggta gatgcgatta accaactgcg tgaaggcttt gaacgtaaag ctgtcgaatt
     6361 ccaggacatc ctgaaaatgg gtcgtaccca gctgcaggac gcagtaccga tgaccctcgg
     6421 tcaggaattc cgcgctttca gcatcctgct gaaagaagaa gtgaaaaaca tccaacgtac
     6481 cgctgaactg ctgctggaag ttaaccttgg cgcaacagca atcggtactg gtctgaacac
     6541 gccgaaagag tactctccgc tggcagtgaa aaaactggct gaagtcactg gcttcccatg
     6601 cgtaccggct gaagacctga tcgaagcgac ctctgactgc ggcgcttatg ttatggttca
     6661 cggcgcgctg aaacgcctgg ctgtgaagat gtccaaaatc tgtaacgacc tgcgcttgct
     6721 ctcttctggt ccacgtgccg gcctgaacga gatcaacctg ccggaactgc aggcgggctc
     6781 ttccatcatg ccagctaaag taaacccggt tgttccggaa gtggttaacc aggtatgctt
     6841 caaagtcatc ggtaacgaca ccactgttac catggcagca gaagcaggtc agctgcagtt
     6901 gaacgttatg gagccggtca ttggccaggc tatgttcgaa tccgttcaca ttctgaccaa
     6961 cgcttgctac aacctgctgg aaaaatgcat taacggcatc actgctaaca aagaagtgtg
     7021 cgaaggttac gtttacaact ctatcggtat cgttacttac ctgaacccgt tcatcggtca
     7081 ccacaacggt gacatcgtgg gtaaaatctg tgccgaaacc ggtaagagtg tacgtgaagt
     7141 cgttctggaa cgcggtctgt tgactgaagc ggaacttgac gatattttct ccgtacagaa
     7201 tctgatgcac ccggcttaca aagcaaaacg ctatactgat gaaagcgaac agtaatcgta
     7261 cagggtagta caaataaaga aggcacgtca gatgacgtgc cttttttctt gtgagcagta
     7321 acttaaaaat aacaatctaa tatcaacttg ttaaaaaaca aggaaggcta atatgctagt
     7381 tgtagaactc atcatagttt tgctggcgat cttcttgggc gccagattgg ggggaatagg
     7441 tattggtttt gcaggcggat tgggggtgct ggttcttgcc gctattggcg ttaaacccgg
     7501 taacatcccg ttcgatgtca tttccattat catggcggtt atcgccgcta tttctgccat
     7561 gcaggttgct ggcggtctgg actatctggt tcatcagaca gaaaagctgc tgcgccgtaa
     7621 cccgaaatac atcacaatcc tcgcaccgat cgtgacctat ttcctgacaa tctttgctgg
     7681 tactggcaac atctctctgg cgacactgcc agttatcgct gaagttgcga aggaacaagg
     7741 cgttaaacct tgccgtccgc tgtctactgc agtggtatcc gcgcagattg cgatcaccgc
     7801 atcgccaatc tcagcggcag tggtttacat gtcttccgtg atggaaggtc atggcatcag
     7861 ctacctccat ctgctctccg tggtcatccc gtccaccctg ctggcggttc tggtgatgtc
     7921 cttcctggtc actatgctgt tcaactccaa actctctgac gatccgattt atcgcaagcg
     7981 tctggaagag ggcctggttg aactgcgcgg tgaaaagcag attgaaatca aatccggtgc
     8041 aaaaacgtcc gtctggctgt tcctgctggg cgtagttggc gtggttatct atgcaatcat
     8101 caacagccca agcatgggtc tggttgaaaa accgctgatg aacaccacca acgcaatcct
     8161 gatcatcatg ctcagcgttg caactctgac caccgttatc tgtaaagtcg ataccgacaa
     8221 catcctcaac tccagcacct tcaaagcagg tatgagcgcc tgtatttgta tcctgggtgt
     8281 tgcgtggctg ggcgatactt tcgtttccaa caacatcgac tggatcaaag ataccgctgg
     8341 tgaagtgatt cagggtcatc cgtggctgct ggccgtcatc ttcttctttg cttctgctct
     8401 gctgtactct caggctgcaa ccgcaaaagc actgatgccg atggctctgg cactgaacgt
     8461 ttctccgctg accgctgttg cttctttcgc tgcggtgtct ggtctgttca ttctgccgac
     8521 ctatccgacg ctggttgctg cggtacagat ggatgacacg ggtacaaccc gtatcggtaa
     8581 attcgtcttc aaccatccgt tcttcatccc gggtactctg ggtgttgccc tggccgtttg
     8641 cttcggcttc gtgctgggta gcttcatgct ataatgacca atcgcggggc gttcacgccc
     8701 cgctttcttt cccgccgact aacatccttc ccccgtccgt tgtatagtga cctctctctt
     8761 gcggttccat ctgttcttgc gaggtgttta tgcttgatga aaaaagttcg aataccgcgt
     8821 ctgtcgtggt gctatgtacc gcaccggatg aagcgacagc ccaggattta gccgccaaag
     8881 tgctggcgga aaaactggcg gcctgcgcga ccttgatccc cggcgctacc tctctctatt
     8941 actgggaagg taagctggag caagaatacg aagtgcagat gattttaaaa actaccgtat
     9001 ctcaccagca ggcactgctg gaatgcctga agtctcatca tccatatcaa accccggaac
     9061 ttctggtttt acctgttaca cacggagaca cagattacct ctcatggctc aacgcatctt
     9121 tacgctgatc ctgctacttt gcagcacttc cgtttttgcc ggattattcg acgcgccggg
     9181 acgttcacat tttgtccccg cggatcaagc ctttgctttt gattttcagc aaaaccaaca
     9241 tgacctgaat ctgacctggc agatcaaaga cggttactac ctctaccgta aacagatccg
     9301 cattacgccg gaacacgcga aaattgccga cgtgcagctg ccgcaaggcg tctggcatga
     9361 agatgagttt tacggcaaaa gcgagattta ccgcgatcgg ctgacgcttc ccgtaaccat
     9421 caaccaggcg agtgcgggag cgacgttaac tgtcacctac cagggctgtg ctgatgccgg
     9481 tttctgttat ccgccagaaa ccaaaaccgt tccgttaagc gaagtggtcg ccaacaacgc
     9541 agcgtcacag cctgtgtctg ttccgcagca agagcagcac accgcgcaat tgcccttttc
     9601 cgcgctctgg gcgttgttga tcggtattgg tatcgccttt acgccatgcg tgctgccaat
     9661 gtacccactg atttctggca tcgtgctggg tggtaaacag cggctctcca ctgccagagc
     9721 attgttgctg acctttattt atgtgcaggg gatggcactg acttacacgg cgctgggtct
     9781 ggtagttgcc gccgcagggt tacagttcca ggcggcgcta cagcacccat acgtgctcat
     9841 tggcctcgcc atcgtcttta ccttgctggc gatgtcaatg tttggcttgt ttactctaca
     9901 actcccctct tcgctgcaaa cacatctcac attgatgagc aatcgccaac agggcggctc
     9961 acctggcggt gtgtttgtta tgggggcgat tgccggactg atctgttcac catgcaccac
    10021 cgcaccgctt agcgcgattc tgctgtatat cgcccaaagc gggaacatgt ggctgggcgg
    10081 cggcatgctt tatctctatg cgttgggcat gggcctgccg ctgatgctaa ttaccgtctt
    10141 tggtaaccgc ttgctgccga aaagcggccc gtggatggaa caagtcaaaa ccgcgtttgg
    10201 ttttgtgatc ctcgcactgc cgatcttcct gctggagcga gtgattggtg atatatgggg
    10261 attacgcttg tggtcggcgc tgggtgtcgc attctttggc tgggccttta tcaccagcct
    10321 acaggccaaa cgcggctgga tgcgcgtggt gcaaataatc ctgctggcag cggcattggt
    10381 tagcgtgcgc ccacttcagg attgggcatt tggtgcgacg cataccgcgc aaactcagac
    10441 gcatctcaac tttacacaaa tcaaaacggt agatgagtta aatcaggcgc tcgttgaagc
    10501 caaaggcaaa ccggtgatgt tagatcttta tgccgactgg tgcgtcgcct gtaaaaagtt
    10561 tgagaaatac accttcagcg acccgcaggt gcaaaaagcg ttagcagaca cggtcttact
    10621 tcaggccaac gtcactgcta acgacgcaca agatgtggcg ctgttaaagc atcttaatgt
    10681 ccttggacta ccgacaattc tcttttttga cggacaaggc caggagcatc cacaagcacg
    10741 cgtcacgggc tttatggatg ctgaaacctt cagcgcacat ttgcgcgatc gccaaccgtg
    10801 aacgacactt gcagtgggaa agacggagga aaatagcgtg caacgtgaag atgtactggg
    10861 agaagccctg aaattattag aattacaagg gattgccaac accacgctgg agatggttgc
    10921 tgaacgtgtg gattatccac tggacgagct acgccgcttc tggccagaca aagaggcaat
    10981 cctctacgat gcgctgcgtt atcttagcca acagatagat gtctggcgtc gtcagctgat
    11041 gcttgacgaa acgcaaaccg ccgaacaaaa gctgctggca cgttatcagg cgttatcgga
    11101 gtgcgttaaa aacaaccgct atccgggctg tctatttatc gctgcctgta cgttttatcc
    11161 cgaccctggc caccctattc atcaactggc cgatcagcaa aaaagcgcgg cctacgattt
    11221 cacccacgaa ctgttaacca cgctggaagt tgacgatccg gcgatggtag caaagcagat
    11281 ggaactggtg ctggaaggct gtttaagccg aatgctggtg aatcgtagcc aggcggatgt
    11341 cgacaccgca catcggctgg cggaagatat cctgcgcttc gcccgctgcc gccagggtgg
    11401 tgcactgacc tgacagaaac acagaaaaga agcgatttgc cgcaatctta agcagttgaa
    11461 tcgcttttac tgaaattagg ttgacgagat gtgcagatta cggtttaatg cgccccgttg
    11521 cccggatagc tcagtcggta gagcagggga ttgaaaatcc ccgtgtcctt ggttcgattc
    11581 cgagtccggg caccaaattc atataaacgg acctccacgg aggtccgttt ttcgtttcag
    11641 gacgccacga tttaagcgtt ctgcctccaa atcaattcta ccgaactcaa ccagattctc
    11701 cccacatcac cagcattttg cgggtatatc ccaattcggg aaaatttgtt cctgagcgat
    11761 agcactgact gacgtgaaat gtcgtgcggc cccgtgatgt tgttgaacgt caaatgacgt
    11821 catcaggagc gtaacgcatc cataaagcac aacaccggtc tgaacggcga ctgatgagat
    11881 tttctgaatg agaacaaaga gaaatgtatc agtccgcttg ctcatgcaaa gactaacaat
    11941 ccattaaaat agtaagcgct ccggacaatt ttccatggat tattttctac acatttttct
    12001 ttggcaaaga tgatgaattt tgatggtaag gaatattact tctggttctc agtaaaatcc
    12061 tttcgtaata ctatgtaatc aagaagttta tggctggtaa aaataacgtc ttgcattcac
    12121 caataacatg taaataaacc catctattga tggtaaaaat aggtcatggc aattatcatt
    12181 gcaccattcc cttttcgaat gagtttctat tatgcaacaa cctgtagttc gcgttggcga
    12241 atggcttgtt actccgtcca taaaccaaat tagccgcaat gggcgtcaac ttacccttga
    12301 gccgagatta attgatcttc tggttttctt tgctcaacac agtggcgaag tacttagcag
    12361 ggatgaactt atcgataatg tctggaagag aagtattgtc accaatcacg ttgtgacgca
    12421 gagtatctca gaactacgta agtcattaaa agataatgat gaagatagtc ctgtctatat
    12481 cgctactgta ccaaagcgcg gctataaatg aatggtgccg gttatctggt acagcgaaga
    12541 agagggagag gaaataatgc tatcttcgcc tccccctata ccagaggcgg ttcctgacac
    12601 aaattctccc tcccacagtc ttaccattca aaacaccgca acgccacctg aacaatcccc
    12661 agttaaaagc aaacgattca ctaccttttg ggtatggttt ttttcctgtt gtcgttaggt
    12721 atctgtgtag cactggtagc gttttcaact cttgatacac gtcttcctat gagcaaatcg
    12781 cgtattttgc tcaatccacg cgatattgac attaatatgg taaataaaag ttgtaacagc
    12841 tggagttccc cgtatcagct ctcttacgcg ataggcgtgg gtgatttggt ggcgacatca
    12901 cttaacacct tctccacctt tatggtgcat gacaaaatca actacaacat tgatgaaccg
    12961 agcagttccg gtaaaacatt atctattgcc tttgttaatc agcgccaata ccgtgctcaa
    13021 caatgcttta tgtcggtaaa attagtagac aatgcagatg gttcaaccat gctggataaa
    13081 cgttatgtca tcactaacgg taatcagctg gcgattcaaa atgatttact ggagagttta
    13141 tcaaaagcgt taaaccaacc gtggccacaa cgaatgcagg agacgctcca gcaaattttg
    13201 cctcatcgtg gtgcgttatt aactaatttt tatcaggctc atgattattt actgcatggc
    13261 gatgacaaat cattgaatcg tgccagtgaa ttattaggtg agattgttca atcatcccca
    13321 gaatttacct acgcgagagc agaaaaagca ttagttgata tcgtgcgcca ttctcaacac
    13381 cctttagatg aaaaacaatt agcagcactg aacacagaaa tagacaacat tgttacactg
    13441 ccggaattga acaacctgtc cattatatat caaataaaag cggtcagtgc tctggtaaaa
    13501 ggtaaaatag atgagtctta ccaggcgata aatactggca ttgatcttga aatgtcctgg
    13561 ctaaattatg tgttgcttgg caaggtttat gaaatgaagg ggatgaaccg ggaagcagct
    13621 gatgcatatc tcaccgcctt taatttacgc ccaggggcaa acacccttta ctggattgaa
    13681 aatggtattt tccagacttc tgttccttat gttgtacctt atctcgacaa atttctcgct
    13741 tcagaataag taactccggg ttgatttatg ctctggaata tttgttgttg agtttttgta
    13801 tgttcccatt ggtataatat gttgcggcaa tttatttgcc gcataatttt tattacataa
    13861 atttaaccag agaatgtcat gcaatccatt gtaaacattg aatgtttatc ttttcatgat
    13921 atcaacttgc gatcctgatg tgttaataaa aaacctcaag ttctcactta cagaaacttt
    13981 tgtgttattt cacctaatct ttaggattaa tccttttttc gtgagtaatc ttatcgccag
    14041 tttggtctgg tcaggaaata gttatacatc atgacccgga ctccaaattc aaaaatgaaa
    14101 ttaggagaag agcatgagtt ctgccaagaa gatcgggcta tttgcctgta ccggtgttgt
    14161 tgccggtaat atgatgggga gcggtattgc attattacct gcgaacctag caagtatcgg
    14221 tggtattgct atctggggtt ggattatctc tattattggt gcaatgtcgc tggcgtatgt
    14281 atatgcccga ctggcaacaa aaaacccgca acaaggtggc ccaattgctt atgccggaga
    14341 aatttctcct gcatttggtt ttcagacagg tgttctttat taccatgcta actggattgg
    14401 taacctagcg attggtatta ccgctgtatc ttatctttcc accttcttcc cagtattaaa
    14461 tgatcctgtt ccggcgggta tcgcctgtat tgctatcgtc tgggtattta cctttgtaaa
    14521 tatgctcggc ggtacctggg taagccgttt aaccactatt ggtctggtgc tggttcttat
    14581 tcctgtggtg atgactgcta ttgttggctg gcattggttt gatgcggcaa cttatgcagc
    14641 taactggaat actgcggata ccactgatgg tcatgcgatc attaaaagta ttctgctcta
    14701 cctgtgggcc ttcgtgggtg ttgagtccgc agcagtaagt actggtatgg ttaaaaaccc
    14761 gaaacgtacc gttccgctgg caaccatgct gggtactggt ttagcaggta ttgtttacat
    14821 cgctgcgact caggtgcttt ccggtatgta tccgtcttct gtaatggcgg cttccggtgc
    14881 tccgtttgca atcagtgctt caactatcct cggtaactgg gctgcgccgc tggtttctgc
    14941 attcaccgcc tttgcgtgcc tgacttctct gggctcctgg atgatgttgg taggccaggc
    15001 aggtgtacgt gccgctaacg acggtaactt cccgaaagtt tatggtgaag tcgacagcaa
    15061 cggtattccg aaaaaaggtc tgct
//
LOCUS       DAEEKQ010000095        15051 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.095, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000095 DAEEKQ010000000
VERSION     DAEEKQ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15051
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.095"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            108..350
                     /locus_tag="KLK57_002726"
     CDS             108..350
                     /locus_tag="KLK57_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893052.1"
                     /translation="MKKVLYGIFAISALAATSVWAAPVQVGEAAGAAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            464..1102
                     /locus_tag="KLK57_002727"
     CDS             464..1102
                     /locus_tag="KLK57_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HBE0893053.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1099..1836
                     /locus_tag="KLK57_002728"
     CDS             1099..1836
                     /locus_tag="KLK57_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HBE0893054.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALEVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1836..3932
                     /locus_tag="KLK57_002729"
     CDS             1836..3932
                     /locus_tag="KLK57_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HBE0893055.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTIGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDAYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(3979..4257)
                     /locus_tag="KLK57_002730"
     CDS             complement(3979..4257)
                     /locus_tag="KLK57_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893056.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4258..4401
                     /locus_tag="KLK57_002731"
     CDS             4258..4401
                     /locus_tag="KLK57_002731"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893057.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPAWVRFVLPDAPPGAAYP
                     AYG"
     gene            4527..4937
                     /gene="psiE"
                     /locus_tag="KLK57_002732"
     CDS             4527..4937
                     /gene="psiE"
                     /locus_tag="KLK57_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HBE0893058.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(4981..6456)
                     /gene="xylE"
                     /locus_tag="KLK57_002733"
     CDS             complement(4981..6456)
                     /gene="xylE"
                     /locus_tag="KLK57_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HBE0893059.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VPLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6828..7718)
                     /gene="malG"
                     /locus_tag="KLK57_002734"
     CDS             complement(6828..7718)
                     /gene="malG"
                     /locus_tag="KLK57_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HBE0893060.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     AIGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7733..9277)
                     /gene="malF"
                     /locus_tag="KLK57_002735"
     CDS             complement(7733..9277)
                     /gene="malF"
                     /locus_tag="KLK57_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HBE0893061.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ECAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGEIGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGADPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLLFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9431..10621)
                     /gene="malE"
                     /locus_tag="KLK57_002736"
     CDS             complement(9431..10621)
                     /gene="malE"
                     /locus_tag="KLK57_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HBE0893062.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVAVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            10986..12101
                     /gene="malK"
                     /locus_tag="KLK57_002737"
     CDS             10986..12101
                     /gene="malK"
                     /locus_tag="KLK57_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HBE0893063.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12173..13513
                     /gene="lamB"
                     /locus_tag="KLK57_002738"
     CDS             12173..13513
                     /gene="lamB"
                     /locus_tag="KLK57_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HBE0893064.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13665..14585
                     /gene="malM"
                     /locus_tag="KLK57_002739"
     CDS             13665..14585
                     /gene="malM"
                     /locus_tag="KLK57_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HBE0893065.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            14814..15051
                     /locus_tag="KLK57_002740"
                     /pseudo
     CDS             14814..15051
                     /locus_tag="KLK57_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         3759 a         3841 c         3942 g         3509 t
ORIGIN      
        1 atcgctaatt ccccgcgccg taattcgcat gctttagttg tgtatactcg atcccgcccg
       61 aaatgttttt gggtaaatct ccattcattc aatgaaggga aattgttatg aaaaaagttc
      121 tgtatggcat ttttgccata tctgcgcttg cggcgacttc tgtgtgggct gcacctgtac
      181 aggtgggcga agcggcaggg gcggcagcaa cgtcggtttc ggcggggagt tcctccgcga
      241 ccagcgtcag caccgtaagc tcggcggtgg gtgtcgcgct cgcggcaacc ggtggcggtg
      301 atggttctaa taccgggacc acaacaacca caaccaccag tacccagtaa taaagtatgt
      361 atccccaaaa taattcgagt cattgcatct gtggcttgaa atatgaaggg attaaccata
      421 accacactcc ggtgtggtta ttctgcccct ctggagaaga gtcgtgaagc gacctgcact
      481 cattcttatc tgcctgttat tacaggcctg ttcagccacg actaaagagc tgggcaattc
      541 actgtgggac agtctgttcg gcacgccagg cgtacagctg acggacgatg atattcaaaa
      601 tatgccctac gccagccagt acatgcagct taatggcggg ccgcaattgt ttgtggtgct
      661 ggccttcgct gaagacggac aacaaaaatg ggtcactcag gatcaagcca ctcttgtcac
      721 acaacatggt cgtctggtga agactttgct tggcggcgac aacctgattg aagtgaataa
      781 ccttgccgcc gacccgctga ttaaacccgc acaaattgtt gatggtgcaa catggacgcg
      841 cacgatgggc tggaccgagt accagcaggt acgctacgcc accgcacgct cagtcttcaa
      901 atgggatggc accgataccg tcaaggtcgg cagcgatgaa accctggttc gcgtgctgga
      961 cgaagaagtc tccaccgacc aggcgcgctg gcataaccgc tactggatcg acagcgaagg
     1021 gcaaattcgc cagtcggaac agtatctcgg cgcggattat ttcccggtga aaaccactct
     1081 catcaaggcg gcaaaacaat gattaaacaa actattgtcg cgttgctttt gagtgtggga
     1141 gcgtcatcgg tcttcgcggc aggaaccgtc aaggtgttca gcaatggcag cagtgaggcc
     1201 aaaacgctaa cgggcgcaga gcatttaatc gatctggtag ggcaacctcg actggcaaac
     1261 agctggtggc ctggtgcggt gattagcgaa gagctggcaa cggcggcagc attgcgtcag
     1321 cagcaggcgt tgctgacacg gctggcagaa cagggcgcag attccagcac cgacgatgcc
     1381 gccgccatta acgccttacg ccagcaaatc caggcattgg aagtgacagg caggcaaaaa
     1441 atcaatcttg atcccgatat cgtccgcgtt gccgaacgcg gtaacccgcc gttgcagggc
     1501 aactacacgc tgtgggtcgg accaccgccg tccacggtca cgttgttcgg gcttatcagc
     1561 catcctggca atcagccatt cactccaggt cgcgacgtgg cgagctatct ctctgaccag
     1621 agcctgctca gcggtgcgga tcgcagctac gcgtgggtcg tttacccgga cggacgcacg
     1681 caaaaagcgc cggtggctta ctggaacaag cgtcacgtag agccgatgcc cggcagcatt
     1741 atttatgttg gcctcgcaga ctccgtctgg agtgagacgc ctgatgccct taacgccgac
     1801 attcttcaga ctctgacgca gcggataccc caataatgaa aaaaagacat ctgcttagct
     1861 tactggcgct gggcattagc acagcttgct acggcgaaac atatcctgcg cccattggtc
     1921 cgtcgcagtc ggatttcggt ggcgtaggat tattacaaac tcccaccgcg cgtatggcac
     1981 gggaagggga gttgagtctg aactatcgcg ataacgatca gtaccgttat tactcagctt
     2041 cagtgcaact cttcccgtgg ctggaaacaa cgctgcgtta caccgacgtg cgcacccggc
     2101 agtacagcag cgtcgaagcg ttctctggcg atcaaacgta taaagataaa gccttcgatc
     2161 tcaaactgcg tttgtgggaa gagagttact ggctgccgca agtggcggtt ggtgcgcggg
     2221 atatcggcgg tacggggctg tttgatgcgg aatatcttgt tgccagcaaa gcctgggggc
     2281 cgttcgattt tacgctcggc ctgggctggg gatatctggg caccagcggt aatgtgaaaa
     2341 atccgctctg ttcagccagt gataaatatt gctatcgcga taacagctac aaacaagcgg
     2401 gatctatcga cggcagtcag atgttccacg gcccggcctc actgtttggc ggcgtggaat
     2461 accagacgcc ctggcaaccg ctgcgcctga aactggagta tgaaggtaat aattatcagc
     2521 aggattttgc cgggaagctg gagcaaaaaa gtaagtttaa cgtcggggcg atttatcgcg
     2581 ttaccgattg ggccgacgtt aaccttagct atgaacgtgg caacaccttt atgtttggcg
     2641 tcacgctgcg caccaacttt aacgatctgc gcccgtccta catcgataac gcccgcccgc
     2701 aatatcaacc gcagccgcag gatgccattt tgcagcactc ggtggtggcg aatcagttaa
     2761 cgttgctgaa atacaatgcc ggacttgccg atccgcagat ccaggcgaaa ggcgatacgc
     2821 tgtatgttac cggcgagcag gtgaaatatc gtgattcgcg cgaagggatc atccgtgcta
     2881 atcggatcgt gatgaacgat ctgccggatg ggatcaaaac gatccgcatt acggaaaatc
     2941 gccttaacat gccgcaggtg acgacggaaa ccgatgtcgc cagcctgaaa aatcatctcg
     3001 ccggagagcc gttgggccac gaaacgacgc tggcgcaaaa acgcgtcgag ccagtggttc
     3061 cgcagtccac cgagcagggc tggtatatcg acaaatcacg ctttgatttc catatcgatc
     3121 cggtgctgaa ccagtcggtc ggtggcccgg aaaactttta catgtatcag ctgggcgtga
     3181 tgggaacggc agatttgtgg ctgacggacc atctgctgac catcggcagc ctgtttgcaa
     3241 atcttgccaa caactacgac aagtttaact acaccaatcc gccacaggac tcgcacttac
     3301 cgcgcgttcg tacccatgtg cgcgagtatg tgcagaacga tgcctatgtg aataacctgc
     3361 aagccaacta cttccagcat ctgggcaacg gcttctacgg tcaggtctat ggaggttatc
     3421 tcgaaaccat gtttggcggt gcgggggctg aagtgttgta tcgtccgctg gacagcaact
     3481 gggcgtttgg tctggatgcc aactacgtta aacagcgcga ctggcgtagt gcaaaagata
     3541 tgatgaaatt caccgactac agcgtgaaaa ccggacatct gaccgcctac tggacgccat
     3601 ctttcgccca ggacgtgtta gtgaaagcca gcgtcgggca gtatctggca ggggataaag
     3661 gcggcacgct ggagatcgcc aaacgctttg atagcggcgt ggtggtgggt ggctatgcca
     3721 cgatcacgaa tgtttcaaaa gaggagtacg gcgaagggga cttcaccaaa ggcgtgtatg
     3781 tctcggtacc gttggatctc ttctcgtctg gcccgacacg cagccgtgcg gcgattggct
     3841 ggacgccgct gacgcgtgac ggtggtcagc aacttgggcg taagttccag ttgtatgata
     3901 tgaccagcga ccgtagcgtc aatttccgct aagtcatggg aaaggtgcca gttttcgcaa
     3961 tcactggcac cctcattctt aatggcctgt tgagccatca ggaacgcagt tcacccccgg
     4021 tttgcgatct ggtgtgtaaa ggcgaaaaca ttctgtcttc ctgtccgtcg atatcggttg
     4081 caggtctggt tgcctgagcg actgggccat cattgggctg gagacattgc tttgcggtag
     4141 gtcggcttta tcagcagtac cgagcggacc agcataagca ggaagaacag agactgataa
     4201 catcaaagca gcaaaataag gcttcatttt taccaccttt atcaggttac gtttcatttg
     4261 ttccagagga acattgtcga tttttcgcgc attgctggtg gctgggaatc acctgaatgg
     4321 gtgatttttg aattaccggc ttgggtgcgg tttgtcttgc cggatgcgcc gccaggcgcg
     4381 gcttatccgg cctacgggta ggtatatccg gctttggtgg aggcgcgctc caaatccagg
     4441 ttgaacaaaa catacacaaa aaatatagat ctccgtcaca tttttgcgtt atacaggaag
     4501 ctcgccactg tgaaggaggt actgctatga cgtcactctc tcgtccgcgc gtggagttta
     4561 tctccaccat tttgcagacc gtactcaatc ttggcctgct gtgcctcggc ctgattttgg
     4621 ttgtcttcct cggcaaagaa acggtgcatc tggcagatgt gctgttcgcg ccagaacaaa
     4681 ccagcaaata tgagctggta gaaggactgg tggtttactt tctctatttc gaatttatcg
     4741 cgctgattgt gaagtacttt cagtccggtt ttcacttccc gctacgttac tttgtctata
     4801 ttgggatcac cgccattgtg cgcttgatca tcgtcgatca taaatcgcca ctggatgtgt
     4861 tgatctactc ggctgcgatc ctgttactgg tgatcactct gtggctgtgt aattcgaaac
     4921 ggctgaagcg ggagtaaaaa gaaagcacgc cgaactggcg cggcgtgctg gacaggaaga
     4981 ttacagcgta gcagtttgtt gtgttttctt cgtttccggt tcccagaggg cttccagctc
     5041 ctcaagggtt ttacctttgg tttccgggac aaatttccac ataaatagtg ctgccagaac
     5101 gcccatacac ccgtaaatcc agtaggagaa accgttgtgg aaatgggcca ccagccagga
     5161 gtttttgtcc atcatcggga aggtccagga gacgaagtag ttcgccagcc actgggccgc
     5221 caccgcgatt gccagcgctt taccacgaat agcattcggg aagatttccg acagcggtac
     5281 ccagcatacc ggaccccagg acatggcaaa ggcagcaaca tagaacagca tcgacagtag
     5341 cgccacaata cccggtgcct gagtgtaaaa cgcggtaccg aggctaaaca taccgattgc
     5401 cattccgagt gcgccgataa tttgcagtgg cttacgacca aatttatcca ccgtcataat
     5461 tgccagaacg gtgaaggtga ggttgataac tccgacaata atggtctgca acagcgcgat
     5521 atccgtgctg gcccccagcg ttttgaacac ttccggcgcg tagtacagca ccacattgat
     5581 gccgacaaat tgctggaaga tggagagcat aacgccgatg acaatcacgc ccacgccaaa
     5641 catcagcaga cgaccaccgg ttttgcggcc atgatccagg gagtgtttaa tttcctgtac
     5701 tgcctgagtt gcaagcgtgt tgcccataat tttgcgcagg atactttccg cctgttcttg
     5761 cttgccgcgt gacatcagcc agcgaggact ttctggcacg gtatacagca gcattaagaa
     5821 cagcagtgca gggatacatt ccgaggcaaa catataacgc cagccgtcag tattcagcca
     5881 gctggcatca ccggaacggg caataaaata gtttacgcag taaactaaaa gttgcccgaa
     5941 aataatcgca aactggttaa atgaaaccag ttttccgcga atatgagctg gagccagttc
     6001 cgcaatatac attggtgaaa gcattgaggc taaaccaacg ccaataccgc caataatacg
     6061 ataaataaca aattccggga cataacctgc cagataaata ggtactgtgt tgtccgggtt
     6121 tatagaggta aaaccaagtt ctggccaggc agaacctaca ccagaaataa aaaacaggac
     6181 agcagcaatc ttaagtgaat cacgacggcc gaagcggtta ctgcaataac caccgagggc
     6241 accgccgatg atgcaaccaa tcagagcgct ggctacgcaa aatcctaaca gggagttggc
     6301 agcggattca cttaagtttt gtggagcaac aaagacggta ttgagtgact caacagtacc
     6361 ggaaataacg gcggtgtcgt agccaaataa taaaccacct aatgtagcga ctaaggtaat
     6421 cgaaaatata taactggaat tatactgggt attcattcag acctgcctta gaccattctg
     6481 atcttttatc agtgcattga tgttggaatt gtcaggcagt taaggcaatt atcatttttt
     6541 acaatgcgtt tacgtaatgt cttttctgtg atcttaattg tgataattat caaaaattga
     6601 tgaaaacaat actattgccg tgactcagag cacgaaagag aattatcgta agtgggaaaa
     6661 caaataacgt aaaaataata agctctatga tggaaatatt aaccggcgaa cgattcagat
     6721 tgcagacgaa agaaaaaaag gcgctccgtg gagcgccgaa taacagtcac aagttgggat
     6781 aacgtaagtt gagggtgcag cggcataaca ttggcagaac aacatcttta acctttcaca
     6841 ccacctgccg tcaggccgtt gaccagccag cgttgagcca gcaagaagac gatggtgatc
     6901 ggtaatgcag acatcacggc agcggcggca aagtcacccc acaggtagtt ttgcgggttg
     6961 aggtattgct gcatccccac ggccagggtg tagctgttta cgtcacgcag taacagcgac
     7021 gcgaccggaa cttcagtaat ggcagcgata aacgacagga tgaataccac cgccagaatc
     7081 ggtactgaca gcggcaacag gacaaggcgg aaggcctgcc acggtgtcgc accatccagc
     7141 gcagccgctt cttccagcga actatcgatg gtttcgaaat agcctttgat ggtccagaca
     7201 tgcagcgcaa tcccacccag atacgcgaaa attacgccgc cgtgagtatt caggccaatg
     7261 aatggaatgt actcacccag acgatcaaac aacgcataca acgcgaccag tgaaagtact
     7321 gccgggaaca tctggaaaat cagcattcct ttcagcagcg tcgctttgcc tggaaagcgc
     7381 atacgggcga aagcgtaggc gcaggtggta gagagcgcga caatgccaat cgcggaaatc
     7441 ccggcgacct ttaccgagtt ccatagccac agcagtaccg ggaatggcgg tggcgtaatg
     7501 cgaccatcag cctgttccac gctaaaacct aaagccagtt tccagtgatc ccaggagatt
     7561 tgctccggga tcaggctgcc gatcgcaaag ttcccctggc gcagtgagat agcgacgacc
     7621 atcagcagcg ggaacataat cgccgcgata aaaagtagca gtagcaggtg agtaataaac
     7681 aaacgtgctt tttgcgattt cggttggacc attgccattt ttgttatctc ccttaatcaa
     7741 acttcattcg cgtggctttc aggttcacta tcgccagcgc acccaccagc aggaagagca
     7801 gcgtggcaat tgctgcagcc agaccgaagt cctgaccccc gccgccttca aaagcgatac
     7861 ggtaggtgta gttaacaagc aggtcggtat aaccggctgg cgtggtcgtg ccaagacgat
     7921 ccgggccgcc gttggttaac agttgaatca gcacgaagtt gttaaagtta aaggcgaagc
     7981 tggcgatcat cagcggcgtc agcggtttaa tcagcagcgg cagcgtaatc ttaaagaagt
     8041 tctggaacgg atctgcgcca tccattgctg aggcttcata caaatcgtcc ggaatcgctt
     8101 tcagcaagcc catgcagagg atcatcatgt acggataacc cagccaggta ttgacgataa
     8161 ttagcatcgt gcgggcggtg gtcggatcgc tgaaccaggc gggcttcacg ccaaacagcg
     8221 cgctcaacat catgttgatt tcaccgaagc tctggttaaa caaccctttg aaaatcaaga
     8281 ttgaaatgaa tgatggcacc gcgtagggca gaatcagcag aacgcgatag accgctttgc
     8341 cgcgcaatgc ttcccactgc accagacatg ccagaaccat gccgaccgcc accgttaaaa
     8401 agacagtgat cagcgagaac accacggtcc agacgaaaat ggcgaggaac ggtttctgaa
     8461 tgccttcgtc ggtgaagacg cgggtaaagt ttttccagcc agtagtcacg gtgtaaccgg
     8521 ggcttagctt ttcatcaccc cagttgccgt cggcggtaat ggactggtaa aagccaattt
     8581 ggttattcgg acgatatttc acgccgctct gattattcgt caacgtgccg tcaccgtcga
     8641 gtgtgtagag cggctgcgtg ccagaaaact ggcgcaggga gctcatcatc actttgttgc
     8701 catccggcag aatggcggta atgtcactca gcgcctgacg attctgggta atcacgcgta
     8761 gattcgcgcg ttcgccttcg ggctgggcgg tcgtttcttt cagttgcagt ttttgttcgc
     8821 cgccaaattt aaacgcgtcg gagaggtaat ttttgccgat ttcgccgtca ctgagcgcca
     8881 gttgccactc atcgcccgcc gggtaaagac caaagttata ggttttgcct gcttgccagg
     8941 agcgatctaa caacacttcc tgcgcacatt caaaagtcag ctggttagtg ctgctgtagt
     9001 tggtgaaggc aatggcgatg gtgcagacca gagggaagag gacgaataat cccattccag
     9061 ccattcccgg gtaaacatag cgccaggcgt aggctttacg attggcgaaa atatacagcc
     9121 ccgctgaact caatatcagc gtggtaatgg cgaacaggta ttccccttgt gcgtacatta
     9181 aaacaacaag gtaacccacc agcaggccga gcagacctag cactgaccat ttcagcgcgt
     9241 cgctttgcca ccaatgtttc tttttaatga catccatggg gttcttcctc attccaggac
     9301 ggataaggct ttcacgcctt atccgacaac aactgcctga tgcgacactg atgcgtctta
     9361 tcaggcctac atgcgtttca gttttgtagg ccggacaagg cgttcacgcc gcatccggca
     9421 tttcacagca ttacttggtg atacgagtct gcgcgtcttt cagggcttca tcgacagtct
     9481 gacgaccgct ggcggcgttg atcaccgcag tacgcacggc ataccagaaa gcggacatct
     9541 gcgggatgtt cggcatgatt tcacctttct gggcgttttc catggtggcg gcaatacgtg
     9601 gatctttcgc caactcttcc tcgtaagact tcagcgccac tgcacccagc ggtttgtctt
     9661 tattgaccgc ttccagacct tcgtcagtca gcagatagtt ttcgaggaac tctttcgcca
     9721 gctctttgtt cggactggcg gcgttaatac cggcgctcag cacgccaacg aacggtttag
     9781 atggttgacc cttgaaggtc ggcagtaccg ttacaccata attcactttg ctggtgtcga
     9841 tgttggacca tgcccacggg ccgttgatgg tcatcgctgt ttcgccttta ttaaaggcag
     9901 cttctgcgat ggagtaatcg gtgtctgcat tcatgtgttt gtttttaata aggtcaacca
     9961 ggaaggtcag acccgctttc gcgccagcgt tatccacggc cacgtcttta atatcgtact
    10021 tgccgttttc atacttgaac gcataacccc cgtcagcagc aatcagcggc caggtgaagt
    10081 acggttcttg caggttgaac atcagcgcgc tcttaccttt cgctttcagt tctttatcca
    10141 gcgccgggat ctcttcccag gtttttggtg ggttcggcag cagatctttg ttataaatca
    10201 gcgataacgc ttcaacagcg atcgggtaag caatcagctt gccgttgtaa cgtacggcat
    10261 cccaggtaaa cggatacagc ttgtcctgga acgctttgtc cggggtgatt tcagccaaca
    10321 ggccagattg agcgtagcca ccaaaacggt cgtgcgccca gaagataatg tcagggccat
    10381 cgccagttgc cgcaacctga gggaatttct cttccagttt atccggatgc tcaacggtga
    10441 ctttaattcc ggtatctttc tcgaatttct taccgacttc agcgagaccg ttatagcctt
    10501 tatcgccgtt aatccagatt accagtttac cttcttcgat tttggcgaga gccgaggcgg
    10561 aaaacatcat cgtcgttaat gcggataatg cgaggatgcg tgcacctgtt tttattttca
    10621 taatctatgg tccttgttgg tgaagtgctc gtgaaaacac ctaaacggac tctagtttct
    10681 ttatacggca acctctttcc atcctccttg cccctacgcc ccaccgttgc tttgtgtgat
    10741 ctctgttaca gaattggcgg taatgtggag atgcgcacat aaaatcgcca cgatttttgc
    10801 aagcaacatc acgaaattcc ttacatgacc tcggtttagt tcacagaagc cgtgttctca
    10861 tcctcccgcc tcctccccca taaaaaagcc agggggtgga ggatttaagc catctcctga
    10921 tgacgcatag tcagcccatc atgaatgttg ctgtcgatga caggttgtta caaagggaga
    10981 agggcatggc gagcgtacag ctgcaaaatg taacgaaagc ctggggcgag gtcgtggtat
    11041 cgaaagatat caatctcgat atccatgaag gtgaattcgt ggtgtttgtc ggaccgtctg
    11101 gctgcggtaa atcgacttta ctgcgcatga ttgccgggct tgagacgatc accagcggcg
    11161 acctgttcat cggtgagaaa cggatgaatg acactccgcc agcagaacgc ggcgttggta
    11221 tggtgtttca gtcttacgcg ctctatcccc acctgtcagt agcagaaaac atgtcatttg
    11281 gcctgaaact ggctggcgca aaaaaagagg tgattaacca acgcgtcaac caggtggcgg
    11341 aagtgctgca actggcgcat ttgctggatc gcaaaccgaa agcgctctcc ggtggtcagc
    11401 gtcagcgtgt ggcgattggc cgtacgctgg tggccgagcc aagcgtattt ttgctcgatg
    11461 aaccgctctc caacctcgat gctgcactgc gtgtgcaaat gcgtatcgaa atctcccgtc
    11521 tgcataaacg cctgggccgc acaatgattt acgtcaccca cgatcaggtc gaagcgatga
    11581 cgctggccga caaaatcgtg gtgctggacg ccggtcgcgt ggcgcaggtt gggaaaccgc
    11641 tggagctgta ccactatccg gcagaccgtt ttgtcgccgg atttatcggt tcgccaaaga
    11701 tgaacttcct gccggtaaaa gtgaccgcca ccgcaatcga tcaagtgcag gtggagctgc
    11761 cgatgccaaa tcgtcagcaa gtctggctgc cagttgaaag ccgtgatgtc caggttggag
    11821 ccaatatgtc gctgggtatt cgcccggaac atctactgcc gagtgatatc gctgacgtca
    11881 tccttgaggg tgaagttcag gtcgtcgagc aactcggcaa cgaaacccaa atccatatcc
    11941 agatcccttc cattcgtcaa aacctggtgt accgccagaa cgacgtggtg ttggtagaag
    12001 aaggtgccac attcgctatc ggcctgccgc cagagcgttg ccatctgttc cgtgaggatg
    12061 gcactgcatg tcgtcgactg cataaggagc cgggcgttta agcaccccac aaaacacaca
    12121 aagcctgtca caggtgatgt gaaaaaagaa aagcaatgac tcaggagata gaatgatgat
    12181 tactctgcgc aaacttcctc tggcggttgc cgtcgcagcg ggcgtaatgt ctgctcaggc
    12241 aatggctgtt gatttccacg gctatgcacg ttccggtatt ggctggacag gtagcggcgg
    12301 tgaacaacag tgtttccaga ctaccggtgc tcaaagtaaa taccgtcttg gcaacgaatg
    12361 tgaaacttat gctgaattaa aattgggtca ggaagtgtgg aaagagggcg ataagagctt
    12421 ctatttcgac actaacgtgg cctattccgt cgcgcaacag aatgactggg aagctaccga
    12481 tccggccttc cgtgaagcaa acgtgcaggg taaaaacctg atcgaatggc tgccaggttc
    12541 caccatctgg gcaggtaagc gcttctacca acgtcatgac gttcatatga tcgacttcta
    12601 ctactgggat atttctggtc ctggtgccgg tctggaaaac atcgatgttg gcttcggtaa
    12661 actctctctg gcagcaaccc gctcctctga agctggtggt tcttcctctt ttgccagcaa
    12721 caatatttat gactatacca acgaaaccgc gaacgacgtt ttcgatgtgc gtttagcgca
    12781 gatggaaatc aacccgggcg gcacattaga actgggtgtc gactacggtc gtgccaacct
    12841 gcgtgataac tatcgtctgg ttgatggcgc atcgaaagac ggctggttat tcactgctga
    12901 acatactcag agtgtcctga agggctttaa caagtttgtt gttcagtacg ctactgactc
    12961 gatgacctcg cagggtaaag gtctgtcgca gggttctggc gtcgcgtttg ataacgaaaa
    13021 atttgcctac aatatcaaca acaacggtca catgctgcgt atcctcgacc acggtgcgat
    13081 ctccatgggc gacaactggg acatgatgta cgtgggtatg taccaggata tcaactggga
    13141 taacgacaac ggcaccaagt ggtggaccgt cggtattcgc ccgatgtaca agtggacgcc
    13201 aatcatgagc accgtgatgg aaatcggcta cgacaacgtc gaatcccagc gcaccggcga
    13261 caagaacaat cagtacaaaa ttaccctcgc acaacaatgg caggctggcg acagcatctg
    13321 gtcacgcccg gctattcgtg tcttcgcaac ctacgccaag tgggatgaga aatggggtta
    13381 cgactacaac ggcgatagca aggttaaccc gaactacggc aaagccgttc ctgctgattt
    13441 caacggcggc agcttcggtc gtggcgacag cgacgagtgg accttcggtg cccagatgga
    13501 aatctggtgg taatagcaaa acctgggccg gataaggcgt ttacgccgca tccggcataa
    13561 aaacaggttg tcattatctg aaaggggcga aagcccctct gattatcggg tttagcgcgc
    13621 tattgcctgg ctaccgctga gctccagatt ttgaggtgaa aacaatgaaa atgaataaaa
    13681 gtctcatcgc cctctgttta tcagcagggt tactggcaag cgcgcctgga attagccttg
    13741 ccgatgttaa ctacgtaccg caaaacacca gcgacgcgcc agccattcca tctgccgcgc
    13801 tgcaacaact cacctggaca ccggtcgatc aatctaaaac ccagaccacc caactggcga
    13861 ccggcggcca acaactgaac gttcccggca tcagtggtcc ggttgctgcg tacagcgtcc
    13921 cggcaaacat tggcgaactg accctgacgc tgaccagcga agtgaacaaa caaaccagcg
    13981 tttttgcgcc gaacgtgctg attcttgatc agaacatgac cccatcagcc ttcttcccca
    14041 gcagttattt cacctaccag gaaccaggcg tgatgagtgc agatcggctg gaaggcgtta
    14101 tgcgcctgac accggcgttg gggcagcaaa aactttatgt tctggtcttt accacggaaa
    14161 aagatctcca gcagacgacc caactgctcg acccggctaa agcctatgcc aaaggcgtcg
    14221 gtaactcgat cccggatatt cccgatccgg ttgctcgtca taccagcgat ggcttactga
    14281 aactgaaagt caaaacgaac tccagctcca gcgtgttggt aggaccacta tttggttctt
    14341 ccgctccagc tccggttacg gtaggcaaca cggcggcacc agctgtggct gcacctgctc
    14401 cggtgccggt gaagaaaagc gagccgatgc tcaacgacac ggaaagttat tttaataccg
    14461 cgatcaaaaa cgctgtcgcg aaaggtgatg ttgataaggc gttaaaactg cttgatgaag
    14521 ctgaacgcct gggatcgaca tctgcccgtt ccacctttat cagcagtgta aaaggcaagg
    14581 ggtaattacg ccccacagtg ctgattttgc aacaactggt gcgtctcctg gcgcaccttt
    14641 ttttatgctt ccttcctggg atatgagcga ttttttatag tacttcactt cttcttcact
    14701 aagaatgtcc attatctcaa tgccttatca gagattcttt tcctttcgcc ggtagtgtct
    14761 ggacgctcag gctacttttc caggttattt tatttctgta ttacagaggt tttatgatag
    14821 gtcatatcac aaattctggc ggcaataact ctttgatgaa acatgatgtg gttcaaggaa
    14881 ataatatagt agatcttgat ttactacgta atttcaatgg ggtgacaggt ttaaacagag
    14941 ataactttat ttatatcagt gatatatttt taaatataaa gcaacggaac gaaaaaatca
    15001 tgcaataaat atgttacgtg aagtctcaat tagtaatgat actataagtg t
//
LOCUS       DAEEKQ010000096        15016 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.096, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000096 DAEEKQ010000000
VERSION     DAEEKQ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.096"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..164
                     /locus_tag="KLK57_002741"
     CDS             <1..164
                     /locus_tag="KLK57_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBE0893066.1"
                     /translation="FWQLAHSSADNFPALTVSHFITANLLPVMLGNIIGGAVLVSMCY
                     RAIYLRQES"
     gene            complement(202..1263)
                     /locus_tag="KLK57_002742"
     CDS             complement(202..1263)
                     /locus_tag="KLK57_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HBE0893067.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1476..2939
                     /gene="bepA"
                     /locus_tag="KLK57_002743"
     CDS             1476..2939
                     /gene="bepA"
                     /locus_tag="KLK57_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HBE0893068.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSQQQARYDARIDQLRQLQERFKPYTKM"
     gene            2960..3319
                     /gene="arsC"
                     /locus_tag="KLK57_002744"
     CDS             2960..3319
                     /gene="arsC"
                     /locus_tag="KLK57_002744"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HBE0893069.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKKNGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3457..4203)
                     /gene="hda"
                     /locus_tag="KLK57_002745"
     CDS             complement(3457..4203)
                     /gene="hda"
                     /locus_tag="KLK57_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HBE0893070.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4253..5542)
                     /gene="uraA"
                     /locus_tag="KLK57_002746"
     CDS             complement(4253..5542)
                     /gene="uraA"
                     /locus_tag="KLK57_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HBE0893071.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5628..6254)
                     /gene="upp"
                     /locus_tag="KLK57_002747"
     CDS             complement(5628..6254)
                     /gene="upp"
                     /locus_tag="KLK57_002747"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HBE0893072.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6579..7616
                     /gene="purM"
                     /locus_tag="KLK57_002748"
     CDS             6579..7616
                     /gene="purM"
                     /locus_tag="KLK57_002748"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HBE0893073.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7616..8254
                     /gene="purN"
                     /locus_tag="KLK57_002749"
     CDS             7616..8254
                     /gene="purN"
                     /locus_tag="KLK57_002749"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HBE0893074.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTIRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8425..10491
                     /gene="ppk1"
                     /locus_tag="KLK57_002750"
     CDS             8425..10491
                     /gene="ppk1"
                     /locus_tag="KLK57_002750"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HBE0893075.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYVASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10496..12037
                     /gene="ppx"
                     /locus_tag="KLK57_002751"
     CDS             10496..12037
                     /gene="ppx"
                     /locus_tag="KLK57_002751"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HBE0893076.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(12076..14319)
                     /gene="pdeF"
                     /locus_tag="KLK57_002752"
     CDS             complement(12076..14319)
                     /gene="pdeF"
                     /locus_tag="KLK57_002752"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HBE0893077.1"
                     /translation="MKLNATYIKMRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYMDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIVPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(14501..14653)
                     /locus_tag="KLK57_002753"
     CDS             complement(14501..14653)
                     /locus_tag="KLK57_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893078.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKTSVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            14671..14862
                     /locus_tag="KLK57_002754"
     CDS             14671..14862
                     /locus_tag="KLK57_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HBE0893079.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
BASE COUNT         3970 a         3824 c         3699 g         3523 t
ORIGIN      
        1 ccttctggca actggcgcac agtagcgcag acaattttcc ggcactgacg gtaagccatt
       61 ttattaccgc caatctgctc ccggtgatgc tgggtaatat tatcggcggt gcagtgctgg
      121 tgagtatgtg ttatcgggct atttatttac gtcaggaatc ctgaaaaatc agcccggcga
      181 aacagttcgt cgggctgaag attattcttg cgctatttgc ccatcgggcc aggcgtggat
      241 cacggctttg atcagcgtcg ccaatggaat agcgaagaat acgccccaga atccccacaa
      301 accaccgaag atcaccaccg ataaaataat caccagcgga tgcaggttaa ccgcttcgga
      361 gaacaacacc ggtactaaca gattaccgtc cagcgcctga ataatcagat acactgcgaa
      421 gcagctccag aattccgtgc ctgcaccaaa ctggaatagc gccacgccaa ccaccggaat
      481 ggtcaccaca aatgcgccaa tgtacggaat aagtaccgag aagccgacca gcaccgccag
      541 cagcagcgaa tagttcagcc caaagagcaa gaaccccagc caagtggcga tccccaccac
      601 gatcatctcc agcactttgc cgcggatata gttggtgatt tgttgattca tctccttcca
      661 cacctgtcct gccagtccac ggttgcgcgg cagcacccga cgaacggcgt tcagcatctg
      721 ctctttgtct ttcagcagga agaagaccat caatggtacc agcaccagat agacggcaat
      781 ggtcagcaaa ccgaccagcg aggcgaggga aattttcacc accgaatcgc ccatggtcag
      841 catccgactg cgcatatttt cggccattgc atcaataatg cccgcgtcca ttaatgccgg
      901 atagcggcgc ggcaacgtgg cggcaaagtc agaaagctta ttgagcatcc ccggcatatc
      961 gcggatcaag tagatgccct gttgccaggc gataggcagt accacgaacg ccatcagtag
     1021 caatataccg acgaaaacca ccaatacaat cgacgtcgcc cagcggcggg agcagccaat
     1081 agattgcagg cgcacggttg gccattccag caaataggcc agcacaatag ccaccagcaa
     1141 cggagcaagc aggccactaa agaaaaagat aatgccaaat ccggcaacta aaataaccag
     1201 caaggcaatc gcttccgggt cgctaaaacg gcggcgatac cattgcatca acatttcgag
     1261 cataaagacc ttccctgaac ctcaagagcg ggattgcgat ccgcaattgt atcgaaatgt
     1321 cacaaaaaag acttcgcttt ttatgacgga ttcaggaaac tgaaaagtca tttgagtggg
     1381 ctaatcttcg ccgttacact caaaggcggc gcggtgggaa caatatttca cagtatcggt
     1441 caaatgacta tcttcagaaa tacaggatag aggttatgtt caggcagttg aaaaaaaacc
     1501 tggttgcaac cctcattgct gctatgacca ttggtcaggt agccccggcg tttgccgaca
     1561 gcgcagacac cttgccggat atgggaacct ccgcaggaag cacgctttcc attggtcagg
     1621 aaatgcagat gggcgactat tatgtccgcc agctacgcgg cagcgcgccg ttaattaatg
     1681 acccgctgtt aacgcaatat attaattcgc tggggatgcg tctggtttcg catgccaatt
     1741 cggttaagac accgtttcat ttttttctga tcaacaacga cgaaattaac gcctttgctt
     1801 tctttggcgg caacgtggtg ctgcactctg ccctgttccg ttattccgat aacgaaagtc
     1861 aactggcttc agttatggcg cacgaaatct cccacgtcac ccaacgtcac ctggcgcgag
     1921 cgatggaaga tcagcagcgc agcgcgccgc tgacctgggt cggcgcgtta ggttctattt
     1981 tactggcgat ggccagtccg caggcgggga tggcggcgct gaccggtaca ctggcgggaa
     2041 cgcgtcaggg gatgatcagt ttcacccagc aaaatgaaca ggaagcggac cgcattggta
     2101 ttcaggtgct gcaacgctcg ggattcgatc cgcaggcgat gccaaccttc ctcgaaaaat
     2161 tactcgatca ggcgcgttac tcctcgcgcc cgccggaaat tttattgact cacccgttgc
     2221 cggaaagtcg tctggcagat gcccgcaacc gtgctaatca gatgcgcccg atggtggtgc
     2281 agtcgtcgga agatttctat ctggcgaaag cgcgcacact ggggatgtat aattccggac
     2341 gtaaccagct caccagtgat ttgctggatg aatgggcgaa aggaaacgtt cgtcagcaac
     2401 gagcggcgca atatggtcgt gctttacagg cgatggaagc caataaatac gacgaggcgc
     2461 gtaaaacgct gcaaccgtta ctggcggcag aacctggcaa cgcatggtat ctcgatctgg
     2521 ctaccgatat cgatcttggg caaaacaaag ccaatgaggc aatcaatcgc ctgaaaaatg
     2581 cccgtgattt gcgcaccaat ccggtgttgc agctcaacct ggcaaacgct tatctgcaag
     2641 gcggtcaacc acaagaagcg gccaatattc tgaatcgcta cacctttaat aataaagatg
     2701 acagcaacgg ctgggattta ctggcacagg cggaagccgc gctaaataac cgcgatcagg
     2761 agctggctgc gcgagcagaa ggttatgcgc tcgccggacg actcgatcag gccatttcgc
     2821 tgttgagtag cgccagttcg caggtgaaat taggcagcca gcaacaagcg cgttacgatg
     2881 cgcgcatcga ccagttgcgc cagctgcagg aacgctttaa gccttatacc aagatgtaat
     2941 tcgcattaaa ggaagaacaa tgaccaaaca ggtaaaaatc taccataacc cacgctgttc
     3001 aaagagccgg gaaacgctga acctgctgaa aaaaaacggc gtggagccag aagtggtact
     3061 ctaccttgag acacccgccg atgcggcaac gctgcgcgat ttgctgaaaa tgctggggat
     3121 gaatagcgcc cgtgaattga tgcgccagaa agaggatctt tataaagaac tgaacctggc
     3181 tgacagttcg cttagcgaag aggcgctgat tcaggcaatg gtggataatc cgaagctgat
     3241 ggagcgcccg attgtggtcg cgaatggcaa agcgcggatt ggtcgtccac cggagcaggt
     3301 actggaaatc gtgggttaag atgtgcccca tcgctagttg aagcacattg ccggatgcga
     3361 cgcttgccat gtcttatccg gcctacgaat actatggatt aacttcgcgt agttcggata
     3421 aggcgttcgc gccgcatccg acaataaaca ccttatctac aacttcagaa tttctttcac
     3481 aaacggaatg gtcagcttac gttgcgcggt aatcgacgca cgatccaact gatccaacgt
     3541 cataaatagc gtgcgcattt ctctgtcgag ccgcttcagc aagaaacgcc ccacatcttc
     3601 cggcagttca aaaccacgca aacgcgcgcg taactgtagc gcctgcaact tatcttcatc
     3661 agaaagtggc tgcaatttgt agatctgccc ccagtcgagt cgcgacgcga gatccggtaa
     3721 tcccagattc aactgccgcg gtggacgatc gccggtgatc aacaatcgtg ttttgcccga
     3781 ttccagaatt cgattgtaga gatcgaaaat cgccatctcc cacaactcat cgcctgcaat
     3841 acactcaatg ttgtcgatac agaccagcga caaatgctcc ataccgtcga gcacttccgg
     3901 aacaaaccag gtgcgtttat ccagcgggac atagcccacc gcatcgccac gctgcgacaa
     3961 ttccgcgcaa gccgcgtgca gcagatggct gcgccccgcg ccttcgcgtg cccagagata
     4021 gatgtaaccg ctatgttcct gacgcagcac gttttgcagc gcagccagta aagaggagtt
     4081 atcccccggc cagaaacttg caaaggtttc gtcgtcagga agataaagtg gcaaagagag
     4141 ctgtgccggt gtgttcagag atacctcaac caggatttca caaaatcgcg agaagtttac
     4201 cacagaatcc catgatgttt gaaccgggca gcaacactgc ccggtcggta cattatttgt
     4261 ctgttatatc cgcgtcttct gcgtccagca ccacttcttc agggcgcagc acactaatca
     4321 gtttgaagat caaacttaag ccgataccga cgatggtcgc cagcgccata cctttcagct
     4381 ccgccgcgcc aatgtttacc ttcgcgccac tgacaccgat gatcaaaatc acggaagtca
     4441 ggatcaggtt ctgtgctttg ttgtaatcca cttttgattc gatcaaaaca cgaataccgg
     4501 aagcaccgat gacaccataa agcagcagcg aaacgccgcc catcaccggc aatgggatca
     4561 tctggatagc ggcagccagt ttaccgacgc aggaaagcag gatagcgaaa atcgccgccc
     4621 caccgataac ccaggtactg taaacacggg tgatcgccat cacgccaatg ttttctccgt
     4681 aagtggtatt tggcgtagag ccaaagaagc cggagatcac agtcgacagg ccgttagcaa
     4741 acatcgaacg gtgcagacct ggatcgcgca gcagatcttt tttgacgata ttagccgtta
     4801 ctaccaggtg ccctacgtgt tcggcaataa ccactaacgc cgctggcaga atagtcagaa
     4861 tggcaaacca ctcgaagcgc ggcgtataga gggttggcag cgcaaaccag tgagcattaa
     4921 taatcggcgt ggtatcgaca attcccattg cgaaagagag cgcgtacccc accagcacgc
     4981 caattaaaat cgggataatt gccaggaaac cacggaacag cacggaacct aaaaccgtga
     5041 ccgccagggt ggtaatagag atgatgatgg ttttggagtc tggcgtttgc ccttcagccg
     5101 ggagtaaacc cgccataccg gcagctacgc ccgccagctc cagaccgatg acggcaacga
     5161 ttgcgcccat tgccgcaggt ggaaacagca cgtccagcca gccggtcccc gctttcttca
     5221 cgataaaaga aaccaggcag aacagcacgc cgcacataat aaagccgccc agcgcgactt
     5281 cataccctaa cggcaacagt aacaataccg gtgaaataaa ggcaaagctg gaaccaagat
     5341 aagccggaat tttcccttta cagatgaaga gatacagcag cgttccaata ccgttaaata
     5401 acagtacagt cgccgggtta atatgaaata agacgggcac caggacggtt gcaccaaaca
     5461 tggcgaacaa atgttgcaaa ctaagcggga ttgtctgtaa aagtggcggt ctttcactca
     5521 ccccgatagc acggcgcgtc atagtattat cctctgtatt atgtgttata ggcgctttac
     5581 tcaaaaaaaa gccgactctt aaagtcggct ttaattattt ttattcttta tttcgtacca
     5641 aagattttgt caccggcatc gccgaggccc ggaataatgt atccgtgctc gttcagtccc
     5701 tggtcaatcg atgcggtata cagttcgacg tccgggtgcg ctttttccag cgcagcgata
     5761 ccttctggcg cagctaccag caccagaact ttgatgctgc tgcagcccgc ttttttcagc
     5821 aggtcgatgg tcgcgataac ggaaccaccg gttgccagca ttgggtcaac gatcagcgcc
     5881 atacgctcat cgatgttaga aaccagtttc tggaagtacg gtaccggctc cagcgtttct
     5941 tcattacggt acataccgac aacgctgatg cgcgcgctcg gaacattttc cagcacaccg
     6001 tccatcatcc ccagaccggc acgcagaatt ggcacaacgg taattttctt acctttgatc
     6061 tggtcgattt ctaccgggcc gttccagcct tcgatagtta ctttttccgt ttcgaggtcg
     6121 gcggtcgctt cgtaagtcag caggctaccc acttcggaag cgagttcgcg aaagcgcttg
     6181 gtgctgatat cttgctcacg catcagtccc agcttgtgtt tgacgagtgg gtgtttgact
     6241 tccacgatct tcatactctt tctcctttga ggggcagcca caaaaaaaat cgacggatta
     6301 tacctccttt cttcaaggcg gcaatattct tttcgttgac tttagtcaaa atgataacgg
     6361 tttgagataa agttatttta tattcaaatg gttatgaaag aagattattc catccgaaaa
     6421 ctaacctttg ccctggcaca agtcttcttt cgccgcgcgt ctggggaaaa gacgtgcaaa
     6481 aaggttgtgc aaagcagtct cgaaaacgtt tgctttccct gttagaattg cgccgaattt
     6541 tatttttcta ccgcaagtaa cgcgtgggga cccaagcagt gaccgataaa acctctctta
     6601 gctacaaaga tgccggtgtt gatattgacg cgggtaatgc tctggttgga agaatcaaag
     6661 gcgtagtgaa gaaaacgcgt cgtccggaag tgatgggcgg tctgggcggc ttcggtgcgc
     6721 tgtgtgcatt gccgcaaaaa tatcgtgaac ccgtgctggt ttccggcact gacggcgtag
     6781 gtaccaagct gcgtctggca atggacttaa aacgtcacga caccattggt attgatctgg
     6841 tcgccatgtg cgttaatgac ctggtggtgc aaggtgcaga accgctgttt ttcctcgact
     6901 attacgcaac cggaaaactg gatgttgata ccgcttcagc ggtgatcagc ggtatcgcgg
     6961 aaggatgtct gcaatcgggc tgttcactgg tgggtggcga aacggcagaa atgccgggga
     7021 tgtatcacgg tgaggattac gatgtcgcgg gtttctgcgt tggcgtggta gaaaaatcag
     7081 aaatcatcga cggctctaaa gtcagcgacg gcgatgtgct gattgcactc ggttccagcg
     7141 gtccgcactc gaacggctat tcgctggtgc gcaaaattct tgaagtcagc ggttgtgatc
     7201 cgcaaaccac cgaacttgat ggtaagccat tagccgatca tctgctggca ccgacccgca
     7261 tttacgtgaa gtcagtgctg gagttgattg aaaaggtcga tgtgcatgcc attgcgcacc
     7321 tgaccggcgg cggcttctgg gaaaacattc cgcgcgtatt gccagataat actcaggcag
     7381 tgattgatga atcttcctgg cagtggccgg aagtgttcaa ctggctgcaa acggctggta
     7441 acgttgagcg ccatgaaatg tatcgcacct tcaactgcgg cgtcgggatg attatcgccc
     7501 tgcctgctcc ggaagtggac aaagccctcg ccctgctaaa tgccaacggt gaaaacgcgt
     7561 ggaaaatcgg tatcatcaaa gcctctgatt ccgaacaacg cgtggttatc gaataatgaa
     7621 tattgtggtg cttatttccg gcaacggaag taatttacag gcaattattg acgcctgtaa
     7681 aaccaacaaa attaaaggca ccatacgggc agttttcagc aataaggccg acgcgttcgg
     7741 ccttgaacgc gcccgccagg cgggtattgc aacgcatacg ctcatcgcca gcgcgtttga
     7801 cagtcgtgaa gcctatgacc gggagttgat tcatgaaatc gacatgtacg cacccgatgt
     7861 ggtcgtgctg gctggtttta tgcgcattct cagcccggcg tttgtctccc actatgccgg
     7921 gcgtttgctg aacattcacc cttctctgct gccgaaatat cccggattac acacccatcg
     7981 tcaggcgctg gaaaatggtg atgaagagca cggtacatcg gtgcatttcg tcaccgatga
     8041 actggacggt ggcccggtta ttttacaggc gaaagtcccg gtatttgctg gtgatacgga
     8101 agatgacatc accgcccgcg tgcaaaccca ggaacacgct atttatccac tggtgattag
     8161 ctggtttgcc gatggtcgtc tgaaaatgca cgaaaacgcc gcgtggctgg atggtcaacg
     8221 tctgccgccg cagggctacg ctgccgacga gtaatgcccc gtagttaaag cgccagctct
     8281 gccgctggcg tttttcaatt cacctgtaaa tcgcaagctc cagcagtttt tttccccctt
     8341 ttctggcata gttggacatc tgccaatatt gctcgccata atatccaggc agtgtcccgt
     8401 gaataaaacg gagtaaaagt ggtaatgggt caggaaaagc tatacatcga aaaagagctc
     8461 agttggttat cgttcaatga acgcgtgctt caggaagcgg cggacaaatc taacccgctg
     8521 attgaaagga tgcgtttcct ggggatctat tccaataacc ttgatgagtt ctataaagtc
     8581 cgcttcgctg aactgaagcg acgcatcatt attagcgaag aacaaggctc caactctcat
     8641 tcccgccatt tactgggcaa aattcagtcc cgggtgctga aagccgatca ggaattcgac
     8701 ggcctctaca acgagctgct gctggagatg gcgcgtaacc agatcttcct gattaatgaa
     8761 cgccagctct ccgtcaatca acaaaactgg ctgcgtcatt attttaagca gtatctgcgt
     8821 cagcacatta cgccgatttt aatcaatcct gacactgact tagtgcagtt cctgaaagat
     8881 gattacacct atctggcggt ggaaattatc cgtggcgata ccatccgtta cgcgctgctg
     8941 gagatcccat cagataaagt gccgcgcttt gtgaatttac cgccagaagc accgcgtcga
     9001 cgcaagccga tgattcttct ggataacatt ctgcgttact gccttgatga tattttcaaa
     9061 ggcttctttg attatgacgc gctgaatgcc tattcaatga agatgacccg cgatgccgaa
     9121 tacgatttag tgcatgagat ggaagccagc ctgatggagt tgatgtcttc cagtctcaag
     9181 cagcgtttaa ctgctgagcc ggtgcgtttt gtttatcagc gcgatatgcc caatgcgctg
     9241 gttgaagtgt tacgcgaaaa actgactatt tcccgctacg actccatcgt ccccggcggt
     9301 cgttatcata attttaaaga ctttattaat ttccccaatg tcggcaaagc caatctggtg
     9361 aacaaaccac tgccgcgttt acgccatatt tggtttgata aagcccagtt ccgcaatggt
     9421 tttgatgcca ttcgcgaacg cgatgtgttg ctctattatc cttatcacac ctttgagcat
     9481 gtgctggaac tgctgcgtca ggcttcgttc gacccgagcg tactggcgat taaaattaac
     9541 atttaccgtg tggcaaaaga ttcacgtatc atcgactcga tgatccacgc tgcgcacaac
     9601 ggcaagaaag tgaccgtggt ggttgagtta caggcgcgtt tcgacgaaga agccaacatt
     9661 cactgggcga agcgcctgac cgaagcaggc gtgcacgtta tcttctctgc gccggggctg
     9721 aaaattcacg ccaaactgtt cctgatttca cgtaaagaaa acggtgaagt ggtccgttac
     9781 gcacacatcg ggaccgggaa ctttaacgaa aaaaccgcgc gtctttatac tgactattcg
     9841 ttgctgaccg cagatgcgcg tatcaccaac gaagtacggc gggtatttaa ctttattgaa
     9901 aacccatacc gcccggtgac atttgattat ttaatggtgt caccgcaaaa ctctcgccgt
     9961 ctgttatatg aaatggtaga ccgcgaaatc gccaacgcgc agcaagggct gcccagtggt
    10021 atcaccctga agctaaataa ccttgtcgat aaaggcctgg ttgatcgtct gtatgtggcc
    10081 tccagctccg gcgtaccggt taatctgctg gttcgcggaa tgtgttcgct gatccccaat
    10141 ctggaaggca ttagcgacaa cattcgtgcc atcagtattg ttgaccgtta ccttgaacat
    10201 gaccgggttt atatttttga aaatggcggc gataaaaagg tctacctttc ttccgccgac
    10261 tggatgacgc gcaatattga ttatcgtatt gaagtggcga caccgctgct cgatccgcgc
    10321 ctgaagcagc gggtgctgga catcatcgac atattgttca gcgatacggt caaagctcgt
    10381 tatatcgata aagaactcag taatcgctac gttccccgcg gcaatcgccg caaagtacgg
    10441 gcgcagttgg cgatttatga ctacatcaaa tcactcgaac aacctgaata accctatgcc
    10501 aatacacgat aaatcccctc gtccgcagga gtttgctgcg gtcgatcttg gttcaaacag
    10561 ttttcacatg gtcatagccc gtgtggtaga tggtgccatg cagattattg gccgcctgaa
    10621 acagcgggtg catctggcgg acggcctggg gccagataat atgttgagtg aagaggcaat
    10681 gacgcgcggt ttgaactgtc tgtcgctgtt tgccgaacgg ctacaagggt tttctcctgc
    10741 cagcgtctgt atagtcggca cccatacgct gcgtcaggcg ctgaacgcca ctgactttct
    10801 gaaacgcgcg gaaaaggtca ttccctaccc gattgaaatt atttccggta atgaagaagc
    10861 ccgtctgatt tttatgggcg tggaacatac ccaaccggag aaaggtcgca aactggttat
    10921 tgatattggc ggcggatcta cggaactggt gattggtgaa aatttcgaac cgattctcgt
    10981 tgaaagccgc cggatgggtt gtgtcagctt tgcccagctt tacttccctg gcggggtcat
    11041 caataaagag aattttcagc gcgctcgtat ggcggcagca caaaaactgg aaactttaac
    11101 ctggcaattc cgtattcagg gctggaacgt ggcaatgggc gcttccggta ccataaaagc
    11161 cgcccatgaa gtgttaatgg aaatgggcga gaaagacggg ataattaccc cggaacgtct
    11221 ggaaaaactg gtaaaagaag ttttacgtca ccgtaatttc gcatcgctga gtttaccggg
    11281 tctttccgaa gagcggaaaa cagtcttcgt tcctggactg gcgattttat gcggtgtgtt
    11341 tgatgcttta gccatccgtg aactgcgcct ttctgacggg gcgttacgcg aaggcgtact
    11401 gtacgaaatg gaaggacgtt tccgtcatca ggatgtgcgt agtcgcaccg ccagcagcct
    11461 cgccaaccag tatcacatcg acagcgaaca ggcccgacgg gtgctggata ccactatgca
    11521 aatgtacgaa cagtggcggg aacagcaacc gaagctggcg catccgcaac tggaggcgct
    11581 actgcgatgg gccgccatgc tgcatgaggt cgggttgaat atcaaccaca gcggtttgca
    11641 tcgccactcc gcttatattc tgcaaaacag tgacttgccg ggttttaatc aggaacagca
    11701 gctgatgatg gcgacactgg tgcgctatca ccgtaaagcg attaaactcg acgatctgcc
    11761 gcgctttacc ttgtttaaga agaaacagtt cctgccactg attcagctat tgcgccttgg
    11821 cgtattactc aacaatcaac gtcaggcaac caccacaccg ccaacattga cactgattac
    11881 tgatgacagt cactggacac tgcgtttccc gcatgactgg tttagtcaga atgcgttggt
    11941 actgcttgat ctggaaaagg agcaagaata ctgggaaggc gtggctggct ggcggttgaa
    12001 aattgaagaa gaaagtacac ctgaaatcgc cgcttaatgc tttgcgggcc gacgagaaat
    12061 gtcggcccgc attattcagg cactttcgcg aatgggttcg atttcattca acgtatcaat
    12121 taacggctgc ggcttaccaa taagataacc ctgcatataa tcgatcccca aagagagcac
    12181 cgcctcgcgg atctcttcgt tttcaacgta ctctgccact acccgcattt tcttcattcg
    12241 cgccagatgg caaatcgacg ccacaatctg ataatccaga ctatttgaca caatattgcg
    12301 gataaagctg ccgtcaattt taagcagatc ggcattcaca tttttaagcc gtgcatagct
    12361 ggcgtagccg gtgccgaaat catcaatcgc aatctggcag cctaattcct gaagatgctg
    12421 caaggtgatt tgcgcctgct taacattggt cagagcatta ctttcggtga cttcaaaaat
    12481 aagttgccac gcttcaatct gatatttagc cagcaactga ctgacttcaa caggaaaacg
    12541 agcctgacat accgaggttg gagacagatt aatagcaaaa cggtgagcgg gcatcttcgc
    12601 tctgttttca gccataaatt gcagcgtatg ctcaatgacc cacatgtcga tactcgacga
    12661 taaaccaaat tcgtgcgcga ccggcaagaa gctatcgggg acgatcagtt catcattctc
    12721 acctttcatg cgcagaagaa tttcatggta aacatcacca cgcataccgg taatcggctg
    12781 ggccatcagg aaaaaatggt tgtgttccag cgcctgctgt agtcgattca tcatcgcgac
    12841 tttatctttc aattcgcgtt gcaaatacat tgccccgcga cgctgcatat tttccggggc
    12901 gttggtcacg atggaaagtt cggcgaccgt atttagctct cccagcagca ggtagatatg
    12961 attcactggc gagcgcacat agcagtaact gacgccaatc tgcggttgca tcggcatccc
    13021 atcccaaaag aaacgaaatt gcttgagatg actatccagt gcggtaatgc gctcctggtg
    13081 cgattctgta ttcagtcgca gcgcgagatc gttacccgaa agctgataaa catcttcacc
    13141 cggttccagc aagggtgaca gccagtgaga aagtttttgc ttgtattgaa tccgcagcat
    13201 gatgccatag ttcttaacca gcatttccat gccagggatg cgtaaataac aaagcgcaga
    13261 ccagggagca tcacgcaacg cgcgattcag ggcgcgaaca tttggcagat gaaccaccgg
    13321 gtccatatac gcaagccgct gaatgcgtct gactaccgct ctctgacggg ttgccagtac
    13381 agccatataa ttgacaataa aagagaatac cagataactg gaggaggtta tggtcagctg
    13441 cgtggtatag ccaggataaa tgggaatgta attttgatag ctgtggatgc tgatcatcaa
    13501 cacgaccgcc cagagcagcg aaatcagctt ataaccatag cgcatcgctc cccacatcat
    13561 caggggcagc aataatgaca aggtataatt ggtgctaaaa attgtgcttt tttcatttaa
    13621 cggcatgcat aacagcaaca ataaagcacc taatgccagt agccagagcg cgaactcttt
    13681 tttggtgact ttggcatcaa cctgctgttt taattgcgaa taatagctac gtaaataaaa
    13741 tggatttcgc actacccgaa tgatgaagta gcacagcggg acaccgatca gattacccac
    13801 cagtaaggcc tgataattga ttaaagtccc gaggttaaaa ggcatgacac cgaccagatt
    13861 ttctctgctc gccagtaatc ctacaaatgc agcaaactgg aaaagtatca gaaacagcgt
    13921 tgcaggaaac acaatctgcc agaatatacg ctgggaaatt aaacgggtat caccatgtga
    13981 aacgttgtta cgccggggag taaagaccct gtaaccgccc caacagagaa tgataatgat
    14041 aataaaatta gccgttaatg atagcgtttc gtaaaaaccc agctctgcat atttgcggac
    14101 aaaaatccct aaggcgatcc ctggcaatgc cgcccagctg aaaaacatca tcatgctgat
    14161 catcagtgcc agaggcagat aaaagagaaa aacctcaccg gaagaaatat gcgcgaaagt
    14221 attaatgtgg gcgaaaatgg gcaagattaa agaaggcagg aacagcggaa gcccccacca
    14281 tttatcacgc atttttatat aagttgcatt cagtttcata gatgctcagc agaatccccc
    14341 acatcctgaa ggaggtgtat tcagacaggc atcccacctg acttcgaatg atgattattc
    14401 atcactatag agagcattga ttctaagtgt catatgaaag ttccaattga tatatatcaa
    14461 acaaaataac cctgattaat gaattattac gtttatcatg ttaattcatc attattacat
    14521 catcattgta aataattaaa ttaacttcca taacattaaa atatgtatcc actgacgttt
    14581 ttttacataa cgaagaattg accattttgt cctgttgtgc cttaatgtaa gtaccgtcca
    14641 cagcgtggga catacttcaa ggaacctttt gtgagtcagg caaccagtat gcgaaaacga
    14701 caccgattta acagtcgcat gacccgtatc gtactgctca tcagctttat cttcttcttt
    14761 ggccgtttta tctactcgtc cgtcggtgcc tggcagcacc atcagagcaa aaaagaagct
    14821 cagcaatcca cactctccgt cgaatcaccg gtacaacgtt agcggttacc ttcttcactt
    14881 tcacagaaca taacggcact tcgctgtcgg atgcttttgc tctttgggat tatcaaagcg
    14941 gcagatattc tttcatctta aattttacgt ctttgtcctg actgatgttt atcctgtttg
    15001 gctgcggaat aaatat
//
LOCUS       DAEEKQ010000097        14677 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.097, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000097 DAEEKQ010000000
VERSION     DAEEKQ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.097"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..1403
                     /locus_tag="KLK57_002755"
                     /pseudo
     CDS             1..1403
                     /locus_tag="KLK57_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-mannosidase"
     gene            complement(1491..2462)
                     /gene="lpxM"
                     /locus_tag="KLK57_002756"
                     /gene_synonym="msbB"
     CDS             complement(1491..2462)
                     /gene="lpxM"
                     /locus_tag="KLK57_002756"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HBE0893080.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(2582..3904)
                     /gene="mepM"
                     /locus_tag="KLK57_002757"
     CDS             complement(2582..3904)
                     /gene="mepM"
                     /locus_tag="KLK57_002757"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HBE0893081.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAAHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(3920..4852)
                     /gene="znuA"
                     /locus_tag="KLK57_002758"
     CDS             complement(3920..4852)
                     /gene="znuA"
                     /locus_tag="KLK57_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HBE0893082.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPGAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            4931..5686
                     /gene="znuC"
                     /locus_tag="KLK57_002759"
     CDS             4931..5686
                     /gene="znuC"
                     /locus_tag="KLK57_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HBE0893083.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            5683..6468
                     /gene="znuB"
                     /locus_tag="KLK57_002760"
     CDS             5683..6468
                     /gene="znuB"
                     /locus_tag="KLK57_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HBE0893084.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(6615..7625)
                     /gene="ruvB"
                     /locus_tag="KLK57_002761"
     CDS             complement(6615..7625)
                     /gene="ruvB"
                     /locus_tag="KLK57_002761"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HBE0893085.1"
                     /translation="MIEADRLISAGTTLPEDGADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(7634..8245)
                     /gene="ruvA"
                     /locus_tag="KLK57_002762"
     CDS             complement(7634..8245)
                     /gene="ruvA"
                     /locus_tag="KLK57_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HBE0893086.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARTDASSETLIREALRAAL"
     gene            complement(8384..8449)
                     /gene="yobI"
                     /locus_tag="KLK57_002763"
     CDS             complement(8384..8449)
                     /gene="yobI"
                     /locus_tag="KLK57_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HBE0893087.1"
                     /translation="MYIFITHLFTEYVILKYLLPI"
     gene            8522..9124
                     /locus_tag="KLK57_002764"
     CDS             8522..9124
                     /locus_tag="KLK57_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HBE0893088.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGELLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(9126..9647)
                     /gene="ruvC"
                     /locus_tag="KLK57_002765"
     CDS             complement(9126..9647)
                     /gene="ruvC"
                     /locus_tag="KLK57_002765"
                     /EC_number="3.1.22.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HBE0893089.1"
                     /translation="MAIILGIDPGSRVIGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(9682..10422)
                     /locus_tag="KLK57_002766"
     CDS             complement(9682..10422)
                     /locus_tag="KLK57_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0893090.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(10451..10903)
                     /gene="nudB"
                     /locus_tag="KLK57_002767"
     CDS             complement(10451..10903)
                     /gene="nudB"
                     /locus_tag="KLK57_002767"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HBE0893091.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQVVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(11021..12793)
                     /gene="aspS"
                     /locus_tag="KLK57_002768"
     CDS             complement(11021..12793)
                     /gene="aspS"
                     /locus_tag="KLK57_002768"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HBE0893092.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTVLAELSIQVVKKAENN"
     gene            13103..13669
                     /locus_tag="KLK57_002769"
     CDS             13103..13669
                     /locus_tag="KLK57_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HBE0893093.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            14024..14272
                     /locus_tag="KLK57_002770"
     CDS             14024..14272
                     /locus_tag="KLK57_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HBE0893094.1"
                     /translation="MRIEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT         3639 a         3742 c         3457 g         3839 t
ORIGIN      
        1 ttgatgaaat atggaaagcg atgctgacct tgcaattcca tgacatcctt cccggctcat
       61 gtatttcaag agtttatcat gaaactgaaa aggaatatct taaactagaa gcaaaaaccg
      121 agaaaattat ttctgatgcg cagtctactt tattaagtaa aattgacact tcttcttata
      181 aagatccgca cattttgttt aataccacct gttttgctcg taacgaatgg atcaatataa
      241 acaacaactg gctcaaagca cgagtaaact cttatggtta tgctgtgatt gttccaaaga
      301 ataagatcgt aaatggtctg aaggcagaat ctcaaagtat tgaaaataat tacattaaac
      361 ttcttttttc agaaagcggt gatttaattt cgctatatga taaaagatat ggtaaagaat
      421 acattactga aaatatgcac agtgagatcc gtgcatacca cgaagatgca ggttttttcg
      481 cggcctggga ttttgcttca aattatcgtg atggggaatc atatgtttta ctcgctgaaa
      541 aaatgacaac tgtgatcagc gggcctaaaa ctacgatgac gctgatttat cactacaatt
      601 cctcttatct tcgtttagcc tttaccctga cacaggatag tccacgcgtt gatgtccaaa
      661 cgtttattga ttggcacgaa cctaatgttt ctctcaaagt aaaattcccg gtgtcagtac
      721 aaacatctct tgcacaatgt caaattcagt tcggtgtaat tgacagacca acgcatagcg
      781 atgattcgtt cgcttttgcc aaagatgaaa taccagcaca tcactgggtc gacctttctg
      841 atcgagaaac tggcatagct ttaatagcaa aaaacaaata tggctatcgt gtcaaagacc
      901 agacgcttga attaactttg ctgcgttcgc agcataaacc tggggaagtt gttaagacag
      961 acaattatga taatttcctg attaataatt ttgcagatat tggtaaacaa aaattcatat
     1021 tctccttatt tcctcatcat ggagattacc gtgtgggtgg tgttattaat caagcctata
     1081 taatgaatca tcccttacag gtcgtccctg tcaaaccgca cccgggtgaa ttaccccctt
     1141 cactatcatt ttttgctatc gatactaaca gcgttatcat cgaaacaata aaattagctg
     1201 aagacggcga cggtataatc gtaagattat atgagagtca tggattagaa atttcagcca
     1261 tgctttcgtg agtatgtaat tatcactacg ttcaatatgt cgatcttcaa gaaaataagt
     1321 tagatgacag tttttattgc aatcaatatt gcaatcttga gttcaaacct tttgaaatta
     1381 tcacgctacg attaactcaa tagcgtacta acctaaaaac tcaaaatagt gggaacactc
     1441 attgagattt tttatcgagt acagcctctc aataatttga gaggcttttt ttatttgata
     1501 ggataaagat ctttacgctt atacggttgg atttcgcccg gtttgcgagt tttcagcaat
     1561 tttaatatcc aggtgtattg ttctggtcgc ggaccaacaa aaatctcgac ttcttcattc
     1621 atccgccgcg caatcgtatg atcatccgcc tctaacagat catccatcgg tgggcgcacc
     1681 tgaatcgtca gacggtgcgt cttgccatca taaatcggaa acagcggaac aacgcgcgca
     1741 cggcacactt tcatcaaacg accaatcgcg ggcaacgtcg ctttataggt ggcaaagaaa
     1801 tccacaaatt cgctgtgctc tgggccatga tcctgatcgg gtaaataata tccccagtac
     1861 ccctgacgta ccgactggat gaatggttta ataccgtcat ttctcgcatg cagacgaccg
     1921 ccaaagcgac gacgcaccgt gttccagaca taatcaaaaa ccgggttgcc ctgattatgg
     1981 aacatcgctg ccattttctg cccttgcgag gccatcagca tggcaggaat atcgacggcc
     2041 caaccgtgcg gcaccagaaa gataactttc tcgttattac gcctcatctc ttcgatgatc
     2101 tccagccctt gccagtcaac gcgcggctga attttctccg gtccgcgtat tgccaactca
     2161 gccatcattg ccatcgcttg cggcgcggtg gcaaacatct catcaacaat cgcttcgcgt
     2221 tcagcttcac tacgttctgg aaagcagagc gacagattga ttaacgcacg acggcgtgag
     2281 ctttttccca gtcgtccggc aaaacgtccc agccgtgcca gaatgggatc acggaacttt
     2341 ggcggcgtta aagcgatacc cgccatcgct gctacaccca gccatgctcc ccagtagcgc
     2401 gggtggcgaa aggatttatc aaactcagga atgtattcgc tattattttt tttcgtttcc
     2461 atgcttttcc agtttcggat aaggcaaaaa tcaatctggt gatagtatag cggcgcaact
     2521 tgccccgcac caaataaaaa aagccgatac tgactgcgta ccggctgcga atggatgtta
     2581 attaatcaaa ccgtagctgc ggcacaatct ctttggcctg cgccaggaat tcgcgacgat
     2641 cggagccggt cagcccttcg gtacgcggca gttttgccgt cagcgggttt acggcctgct
     2701 ggtttatcca tacttcatag tgcagatgcg gcccggttga acgtccggta ttaccggaaa
     2761 gcgcgatacg gtcgccacgt ttcaccttct gtcccggttt caccagaatc ttgcgcaagt
     2821 gcatataacg cgtggtgtag ctgcgaccat gacgaatagc cacataataa cctgctgcgc
     2881 cactgcgttt ggcaaccacc acttcaccgt cacccactga aagcactggc gtaccctgcg
     2941 gcatggcgaa atcaacacct ctgtgtgctg caacgcgacc ggtcaccgga ttagtacgac
     3001 gcgggttaaa gttagacgag atacggaact gtttcgccgt cgggaatcgc aagaatcctt
     3061 tcgccagacc agtaccgtta cgatcgtaga atttgccatc ttcagcgcgg atagcgtaat
     3121 aatctttacc ttctgaacgc aaacgtacgc ccagcagctg gctttgctca cgtttaccat
     3181 caagcatttc tcgagacatt aacaccgcaa attcatcgcc ttttttcagt ttgcggaaat
     3241 ccatttgcca ctgcatggct ttaatcactg cgctcacttc ggcgctggtt aaaccggcgt
     3301 ttctggcgct ggcaacaaag ctccccccga cggtaccttt cagcagattg ttaacccact
     3361 ctccttgctg catttcgctg gtcattttaa aaccgttagc ggcagtacgg tcataggttc
     3421 gggtttcacg acgagacact tcccaggtga ggcgctgcag ttcgccgtcc gcggttaatg
     3481 tccaggagag ttgttgaccg attttcaggt tacgcaattc tttgtcggcc gcagccagtt
     3541 gggtgatatc acccatatca ataccatact gattgagaat gctgcttagc gtatcgccag
     3601 tggaaacaac atattcatgc acgcccgctt caccggcgat tttgtcatcc agttcgtcct
     3661 ggggaatggc ttcatcttct tgtgcagctt gatcaatcgg ctcactggct tcaggtaaga
     3721 gcgaacgaat ttcgttctgt tccagctcaa tggttttgac aattggcgtg gcatcgcggt
     3781 gataaacata gggccgccag acagcgacgg ccagagtaag aacggtgagc gaccccaaca
     3841 taacgcggtg tggtcgcggt aaattattaa acgccagggc gacagagcgg gctatctgtt
     3901 gcacgtaatc acttcctcat taatctcctt tcaggcagct cgcatactgg ttggctaatt
     3961 gactcaggaa ttctgaatag cttgttttac ccagtttgat attcgtcccc aggggatcca
     4021 acgttcccat acgaacggat gtccctcgtg cgacgctctc aacgaccgct ggcctgaact
     4081 gtggctcagc aaaaacgcag gttgcttttt gctcaaccaa ctgtgttctt atttcatgta
     4141 aacgctgcgc gccaggttga atctcagggt taacggtaaa atgaccaagc ggtgtcagtc
     4201 cgaactgttt ttcgaaatag ccgtaagcat cgtgaaaaac gaaataacct ttccccttga
     4261 gcggcgcgag ctcgttacca acctgcgttt cggttgaggc taattgtgcc tcaaaatcct
     4321 tcaggttggc gtcaagtttg gctcgacttt gcggcataag ttccactaat tttccatgga
     4381 ttgcaaccgc tgtagcccgc gctatctctg gggaaagcca aagatgcatg ttgaaatcgc
     4441 cgtgatggtg atcttcgtca cttttttccg cgtggtcgtg atcatcatca tcgccgtgaa
     4501 tacttttcat cagtagcggt ttcacatctt caagctgcgc aatcgttacc tgcttcgctc
     4561 ctggtaattt gcttaccggt ttttgcataa acgcttccat ctccgggcca acccaaacga
     4621 ctaagtccgc gttctgtaag cgttttacat ccgatgggcg cagtgaataa tcatgttctg
     4681 aagcgccgtc aggaagtaaa acctctgttt ctgttacccc atcagcaatg gcagaagcga
     4741 tgaacccaac gggtttaagc gaagcgacaa cggcggcatc tgcggcctgt gttgcacctc
     4801 cccagagagc ggcggataat gctgcgaaaa gaagcgtttt tttatgtaac ataatgcgac
     4861 caatcatcgt aatgaatatg agaagtgtga tattataaca tttcatgact actgcaagac
     4921 taaaattaac atgacaagtc tggtttcctt ggaaaatgtc tcggtttctt ttggccaacg
     4981 ccgcgtcctc tctgatgtgt cgctggaact taaacctgga aaaattttga ctttacttgg
     5041 gccaaatggc gcaggtaagt cgacactggt acgggtagtg ctcgggctgg taacacccga
     5101 tgaaggggtt atcaagcgca acggaaaact gcgcatcggc tatgtaccgc agaagctgta
     5161 tctcgacacc acgttgccac tgaccgtaaa ccgtttttta cgcttacgcc ctggtacaca
     5221 taaagaagat attttgcctg cactgaaacg tgtccaggcc gggcacctga ttaacgcacc
     5281 gatgcaaaag ctctctggtg gcgaaacgca gcgtgtactg ttagcgcgag cattgttaaa
     5341 tcgcccgcaa ttattagtgc tggatgaacc cactcaaggc gtggatgtaa atggccaggt
     5401 ggcgttatat gaccttattg accaactgcg tcgtgaactg gattgtggcg ttttaatggt
     5461 ttctcacgat ctgcatctgg tgatggcaaa aaccgatgaa gtgctgtgcc tgaatcacca
     5521 catttgttgt tccggcacac cggaagttgt ttccctgcat ccggagttta tttcaatgtt
     5581 tggtcctcgt ggtgctgaac aactgggtat ctatcgccat catcataacc atcgtcacga
     5641 tttacaggga cgaattgttt tgcgtcgggg aaatgatcgc tcatgattga attattattt
     5701 cccggttggt tagccgggat catgctcgcc tgtgccgcgg gtccgctggg ttcgtttgta
     5761 gtctggcgtc gtatgtctta tttcggtgat acgctggctc atgcctcatt acttggtgtc
     5821 gcgtttggtt tgttgctgga cgtgaatcca ttctatgcgg tgattgccgt tacgctgctg
     5881 ctggcgggcg gtctggtatg gctggagaag cgtccacagc tggcgatcga cacgttatta
     5941 gggattatgg cgcacagtgc cctgtcgctg ggcctggtgg tcgttagtct gatgtctaat
     6001 attcgtgttg atttgatggc ttacctgttc ggtgatttgc tggcagtgac gccagaagat
     6061 ctcatctcta ttgcgattgg cgtggtcatc gtggtggcta ttttgttctg gcaatggcgc
     6121 aatttgctgt cgatgacgat tagcccggat ctggcgtttg ttgatggtgt gaaattacag
     6181 cgcgtgaaat tgttgttgat gctggtgacg gcattgacga ttggtgtagc gatgaaattt
     6241 gtcggtgcgt tgattattac ttcgttgctg attattcctg cagctactgc gcgtcgcttt
     6301 gcccgtacgc cggaacagat ggctggtgtc gctgttttgg tggggatggt ggcagtaact
     6361 ggcggtttaa ccttttccgc gttttacgat acgccggcgg gtccgtcggt agtgctatgt
     6421 gctgcactgt tatttattat cagtatgatg aaaaagcagg ccagctaatc tgtcgctgaa
     6481 cacatttgtc ggatgcggcg cgagcgcctt atccgaccta cggttcgcta tctctggtag
     6541 gccggataag acgcgaatag cgtcgcatca ggcacactgc caatgtcgga tgcggtgcga
     6601 gcgaccaatc cgacttacgg catttctggc ggcgttatgc caaagtgatt ccacgcccgc
     6661 gtcgtcgcca tacgcccacg cggtgtacgc tgcaaaaagc cttgctgaat cagataaggc
     6721 tccagcacat cctcaatggt ttcacgttct tcgccaatgg ctgccgccag gttatccaga
     6781 cctaccggcc caccaaagaa cttatcgatt accgccagca acaatttgcg gtccatataa
     6841 tcgaaacctt cagcatcgac attcaacata tccagcgcct gagcagcgat atctgccgag
     6901 atggtgccat cgtgcttcac ttcggcgaaa tcacgcactc gacgcagcag acggttggca
     6961 atacgtggcg taccgcgagc gcgacgagca acttccagcg cgccgtcatc actcatctca
     7021 agccccataa agcgtgcgct gcgactgacg atatattgca gatccggcac ctgataaaac
     7081 tccagacgtt gcacaatacc aaaacggtcg cgcaacggtg atgtcagcga acctgcgcgc
     7141 gtggttgcac caatcagggt aaacggcggc aaatcaattt taatggagcg tgccgccgga
     7201 ccttcaccaa tcatgatatc cagttggtag tcttccattg ccgggtacag cacttcttca
     7261 acaactggcg atagacggtg gatctcatca ataaacagca cgtcatgcgg ttcaaggtta
     7321 gtgagcatcg ctgccagatc gcccgccttt tccagcaccg gaccagaagt cgtgcgtaaa
     7381 ttaacgccca tttcattggc gacaatgttg gcaagcgtag ttttacccaa ccccggagga
     7441 ccaaaaatca acagatgatc gagggcatcg ccgcgcagtt tcgctgcttt gatgaaaatc
     7501 tccatctgtg aacgaacctg cggctgacca acatactctt ccagtaattt agggcgaatg
     7561 gcgcgatctg ccccatcttc cggcaaagtg gtaccggcag aaatcagacg gtctgcttca
     7621 atcatccttt acctcataac gcggcgcgta gggcttcgcg aattaatgtt tcactgctgg
     7681 cgtcagtgcg agcgattttg ctcaccatgc ggcttgcttc ttgtggttta tagcccagcg
     7741 ccaccagcgc ggcaaccgct tcctgttcag catcgtcggt tgccgggctg gcaggcgacg
     7801 tgagcaccag atcggcggct ggcgtaaaga gatcgccgtg caaaccttta aatcggtctt
     7861 tcatttcgac aattaaacgc tcggcggttt ttttaccaat gcccggcagt ttcaccagtg
     7921 ctcccacttc ttcacgttca acggcattaa cgaactgctg cgctgacatt ccggagagga
     7981 tcgccagcgc caacttcggg ccgacgccgt tggttttgat taactcttta aacaacgtgc
     8041 gctcttgttt attgttaaaa ccgtacagca gttgcgcgtc ttcacgcacc acaaagtggg
     8101 tgaaaacgat cgcttcctga cccgcttcag ggagttcata aaaacaggtc atcggcatat
     8161 gcacttcata gcctacgccg cccacttcaa ttaacaccag cgggggttgt ttttcaatga
     8221 tgatgcctct gagtctgcct atcacatgac gctcctgcgt aatgaatcaa agataatgct
     8281 gtatgataaa aaaatgctgg atagatatcc agcgaaggat gaagaaaact tgcgaggtgt
     8341 ctcgatgaac tgaaaaatgg cacagtataa tttattctac aggttatatt ggaagcaaat
     8401 attttaatat tacatattca gtgaataaat gcgtaataaa aatatacatt gcgcctcctg
     8461 aaaaaaaata aattttttat gctattacgt atattcgtat ctatttcaat ggaatgacaa
     8521 cgtgaatatt aattaccctg ctgaatatga aattggagat atcgtcttta catgtatagg
     8581 tgctgcctta tttggtcaaa tatcagctgc atcaaattgc tggagtaatc acgtcgggat
     8641 cattatcggt cataacggtg aagacttcct ggttgcagaa agccgggttc ccctttcaac
     8701 catcaccacg ctatctcgtt ttattaaacg ttcgtctaat caacgctatg ctataaagcg
     8761 attagacgct gggctgacag aacaacaaaa acaacgaatt gttgaacagg ttccctcccg
     8821 gctacgcaaa ctttaccaca ccggttttaa atacgaatct tcgcgccagt tctgttcaaa
     8881 atttgttttt gatatttata aagaagcgct atgcattccg gtgggtgaaa tagaaacgtt
     8941 tggagaactg ttaaatagca atccgaatgc aaaactcact ttctggaaat tctggttctt
     9001 aggttctatt ccgtgggagc gtaaaaccgt cacgccagcc agtttgtggc atcatccggg
     9061 tctggtgttg attcacgcgg agggagttga aacgcctcag cctgaactga ccgaggcggt
     9121 ataacttaac gcagtcgccc tctcgccagg ttcagccgcg attcgctcat ctgcattgca
     9181 ttctggctga cgtgacaatg ggtaatcgcg atagctaacg catcggcggc atccgcctgt
     9241 ggattagcgg gcagtttcag caaggtgcgg accatatgct gcacctggct tttttcggca
     9301 ctaccaatac ctaccaccgt ttgctttacc tgacgtgccg cgtattcaaa taccggcaat
     9361 tcctgattca ccgccgccac aatcgccacg ccgcgcgcct gccccagttt cagagccgag
     9421 tcggcatttt tggccataaa gacttgttca atggcgaaat aatctggctg gaactgggtg
     9481 atgatttccg tcacgcccgc atagatgagc ttcagacgag acggtaaatc atccactttg
     9541 gtgcgtatgc atccgctacc caggtaggac agttgcctgc ctacctggcg gatgacacca
     9601 tagccgatca cgcgcgaacc cgggtcaatg ccgagaataa tagccatcac gcgtctccgt
     9661 tttgctgttt agcaggcccc atcagagagt tgctgcaacc tcatcagaga tttcaccgtt
     9721 atggtagact tcctgcacgt cgtcgcaatc ttccagcata tcgatcagac gcatcagttt
     9781 cggtgcggtt tctgcatcca tatcagcttt ggtggacggg atcatggaaa cttccgcgct
     9841 gtctgctttc agacctgccg cttccagagc gtcgcgcact ttacccattt cttcccatgc
     9901 agtgtagacg tcaatcgcgc cgtcatcata ggtcacaacg tcttcagcac ctgcttccag
     9961 tgctgcttcc ataatggtgt cttcatcgcc tttctcgaag gagatcacgc cttttttgct
    10021 gaacagatag gcaacggaac catcagtacc gaggttaccg ccacatttgc taaatgcatg
    10081 acgcacttca gcaacggtac ggttgcggtt gtcagacaga cattcaatca tgattgccgt
    10141 gccgccagga ccgtaacctt cgtagatgat ggtttccatg tttgcatcat catcaccgcc
    10201 cacaccacgt gcaattgcgc ggttcagtgt gtcacgggtc atgttgttag acagtgcttt
    10261 atccaccgcc gcacgcagac gcgggttagc gtccggatcg ccaccgccca gcttagccgc
    10321 ggttaccagc tcacgaatga ttttagtgaa gattttaccg cgcttagcat cctgcgcagc
    10381 tttacgatgt ctggtgttgg cccatttact atgacctgcc ataaaaatat ctccagatag
    10441 ccctgcctgt tcaggcagcg ttaattacaa actgttcaat cgcctgccgg ttgctccagg
    10501 acttagtgag cgccgcggca gcaggcgcat caagccactt gtaagccaga tgttcagtga
    10561 agactacctg gcgttcgtgc gggagcgcaa gacagaacca tgattccgta ttacgcgtca
    10621 cgcccggcgc atagcgatga cgtaaatgtg aaaaaatttc aaactctacc gtgcgctgac
    10681 agtcaattaa ggtcagttgt tcagcgacaa catcaatggt gacctcttcc tttacttcgc
    10741 gcatggcagc ttgcggcgcg gtttcaccct cttccacgct gccggttacc gactgccaga
    10801 aatcgggatc gtcacgccgc tgcaacatca gcacccgttt cgtatcttgt gcatagatga
    10861 ccactaagat cgaaacggga cgcttataca ctttatcctt cacgctgcct cttcgttgac
    10921 tgccttcgct catcccattc acatagttat ctatgctcat gggagttcac tcagttgccg
    10981 ccttgatgca acgcgaatga tttcgtgtat ttgagtcata tcagttattc tcagccttct
    11041 tcacaacctg aatgctcagc tcagccagta cagtcgggtt agcaaagctc ggtgcttcag
    11101 tcatcagaca cgctgccgcc gtggttttcg ggaaggcgat aacgtcacgg atattgtcgg
    11161 tgccagtcag cagcatggtc agacggtcaa gaccgaatgc cagacctgcg tgcggcggag
    11221 taccgtattt cagggcgtcg agcaggaagc cgaatttctc gcgctgttcc tcttcgttga
    11281 tacccagaat accaaacacc gtctgctgca tatcaccatt atggatacgt actgaaccac
    11341 cgcccacttc gtaaccattg atgaccatat cgtaagcgtt cgccaccgca ttttccggtg
    11401 cagctttcag ttctgcagca gtcatgtctt tcggtgaggt gaacggatgg tgcattgccg
    11461 tcaggccgcc ttcaccgtcg tcttcaaaca tcgggaagtc gataacccac agcggtgccc
    11521 atttgctttc gtcggtcaga ccaaggtctt tacccacttt caggcgcagt gcgcccatcg
    11581 cgtcggcaac aattttcttg ttgtcggcac cgaagaaaat catatcgcca tcttgcgcgg
    11641 cagtacgatc caggatggct tcgatgattt ctgcattaag gaacttcgct accgggctgt
    11701 tgataccttc cagacctttc gcgcgttcgt taactttgat gtaagccaga cctttcgcgc
    11761 cgtagatttt aacgaagtta ccgtattcgt cgatctgctt acgggtcagc gatgcgccac
    11821 ccggaacacg tagagcggca acacggcctt tcggatcgtt cgccggcccc gcaaatactg
    11881 caaactcaac agatttcagc agatcggcaa cgtcggtcag ttccatcggg ttacgcagat
    11941 ccggtttatc agaaccataa cggcgttctg cttccgcaaa ggtcattacc gggaaatcgc
    12001 ccagatccac gcccttcact tccagccaca aatgacgcac cagcgcttcc atcacttcac
    12061 gcacttgcgg cgcggtcatg aaagaagttt ccacatcgat ctgagtaaat tcaggctgac
    12121 gatcagcgcg caggtcttcg tcacggaagc atttaacgat ctgatagtaa cggtcaaaac
    12181 cggacatcat cagcagctgt ttgaacaact gtggggattg cggcagtgcg tagaatttac
    12241 ctttgtgcac acgagaaggc accaagtagt cacgcgcgcc ttccggcgtg gctttggtca
    12301 gcatcggagt ttcgatgtcg aggaagccgt ggtcatccat aaaacggcgc accaggctgg
    12361 tgattttagc gcgggttttc aggcgctgcg ccatttccgg gcgacgcagg tcgaggtagc
    12421 ggtatttcag acgcgcttct tcggtgttga cgtggttaga gtcaagcggc agaacatctg
    12481 cgcggttgat gatagtcagc gaggacgcca gcacttcaat ttcgccggtc gccatatcgc
    12541 ggttaatatt tttttcgtca cgcgcacgta cggtgcccgt aacctgaatg cagaactcat
    12601 tacgcagttc agaggccagc tttaacgcgt ccgcacgatc cggatcgaaa aatacctgca
    12661 cgataccttc gcggtcgcgc atatcgatga agatcaggct accaagatca cgacgacggt
    12721 tgacccaacc acacagagtc acctgctgcc ccacgtggga caaacggagc tgtccacaat
    12781 attctgtacg catgagatat cccttaactt agctgccggc ggatgccccc tgctgcgcag
    12841 gtgaccaagt cgcagcgtta gctgtatgtc acaactgaat gaaaaaaggc ggctattata
    12901 ctggaaattc tgccgcaccg taagagcctg gcccgcgctg taacgcctcg ttaccacttt
    12961 atatcgggcc tgaaatcaga ctctacgcca gtttgctata aaggtgttgc ccgaactcat
    13021 aaaaattaac aaaatttgtc gttccgccat cggctaatcg cattaaggtg tgaggcacga
    13081 ttttgttttt tcaggagtca gcatgcttga acttaatgct aaaaccactg cgttggtggt
    13141 gatcgattta caagaaggca tcttaccttt tgccggtggt ccacatactg ccgatgaggt
    13201 agttaatcgc gccgggaagc tggcggcgaa atttcgagcc agcggtcagc ccgtgtttct
    13261 ggtgcgagtt ggctggtctg ccgattacgc cgaagcatta aaacagccgg ttgatgcccc
    13321 ctcccccgct aaagtgttgc ccgaaaactg gtggcaacat ccagctgcat taggtgcaac
    13381 tgacagtgat atcgaaatca tcaaacgtca gtggggtgcg ttttacggta cggatctgga
    13441 gttgcaactg cgccgccgag gtatcgatac aatagtgtta tgtgggatct cgaccaatat
    13501 cggtgttgaa tccaccgccc gcaatgcctg ggaacttggc tttaatctgg tgattgccga
    13561 agacgcctgt agtgccgcca gcgccgagca acacaataac agcattaatc atatctaccc
    13621 gcgcatcgcc cgtgtgcgta gcgtggaaga gatccttcac gcgttatgat ttacatcggt
    13681 ctaccgcaat ggtcgcatcc taaatgggtg cggttgggga tcaccagcct tgaagagtat
    13741 gcccgccact ttaactgcgt gatacgggat ttttaaaaat cactaaagaa cgcccaagag
    13801 catgtgtttt ctttagaata atcaaagcgt tggattgcca tgccatatga aaatcggcaa
    13861 agttcatatg taacaaagtg tcccaatcat gccccaaaat gccccacaac aaatattttt
    13921 gccccatgca tgccccaaaa aatcactttc cctcactctt gacactgttt atccatacag
    13981 ttaaaaataa tactgtataa aaacacagtg tagagggatt tttatgcgta ttgaaatctg
    14041 catagccaaa gagaaaatga ctaaaatgcc aaccggtgct gtggatgcgt taaaggaaga
    14101 attaacccga cgcatcagta aacgttatga cgatgtagag gtgatcgtaa aagccaccag
    14161 caacgatggc ctttctgtta cgcgcaccgc cgataaagat tcagctaaaa cttttgttca
    14221 ggaaactctg aaagatacct gggagtctgc tgacgagtgg tttgttcact aattagcacg
    14281 taaaatcagt aacggctgga aatcattcaa tactcgcact atcggaagtt caccagccag
    14341 ccgcagcacg ttcttgcata cgacgtgtct gcggtctcaa ctccgcccgc ccccgccaat
    14401 catgattggg cagtaccagt acagcactag caaagacagg aaattagcag tctcagcaaa
    14461 acaccgacca gacggtgaga agacaaaaag atacgcaaag atgccgcggt tccagaagaa
    14521 taaaaaaccg ctaatggaca gcctccttaa cacagttttt tatatacatg attcactgat
    14581 accagtggca ttaatagcta ttatatagct tcagctcctt ttttcatacc ttaaggaaaa
    14641 attgccccct cccccaaaaa gtatcgtttt tttacat
//
LOCUS       DAEEKQ010000098        14422 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.098, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000098 DAEEKQ010000000
VERSION     DAEEKQ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14422
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.098"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            270..1388
                     /locus_tag="KLK57_002771"
     CDS             270..1388
                     /locus_tag="KLK57_002771"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HBE0893095.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWADD"
     gene            1454..1702
                     /locus_tag="KLK57_002772"
                     /pseudo
     CDS             1454..1702
                     /locus_tag="KLK57_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
     gene            1767..2135
                     /locus_tag="KLK57_002773"
     CDS             1767..2135
                     /locus_tag="KLK57_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HBE0893096.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2229..2882
                     /gene="nfsB"
                     /locus_tag="KLK57_002774"
     CDS             2229..2882
                     /gene="nfsB"
                     /locus_tag="KLK57_002774"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HBE0893097.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            2990..4237
                     /gene="ybdG"
                     /locus_tag="KLK57_002775"
     CDS             2990..4237
                     /gene="ybdG"
                     /locus_tag="KLK57_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HBE0893098.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="KLK57_002776"
     CDS             complement(4305..5681)
                     /gene="pheP"
                     /locus_tag="KLK57_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HBE0893099.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPDKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="KLK57_002777"
                     /pseudo
     CDS             complement(5783..8926)
                     /gene="cusA"
                     /locus_tag="KLK57_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
     gene            complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="KLK57_002778"
     CDS             complement(8938..10161)
                     /gene="cusB"
                     /locus_tag="KLK57_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HBE0893100.1"
                     /translation="MKKIALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGNEQRVITVDADGRFVPKRVAVFQASQGVTALHSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATNAH"
     gene            complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="KLK57_002779"
     CDS             complement(10177..10509)
                     /gene="cusF"
                     /locus_tag="KLK57_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HBE0893101.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="KLK57_002780"
     CDS             complement(10533..11915)
                     /gene="cusC"
                     /locus_tag="KLK57_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HBE0893102.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRTAMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQQ"
     gene            12072..12755
                     /gene="cusR"
                     /locus_tag="KLK57_002781"
     CDS             12072..12755
                     /gene="cusR"
                     /locus_tag="KLK57_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HBE0893103.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            12745..14193
                     /gene="cusS"
                     /locus_tag="KLK57_002782"
     CDS             12745..14193
                     /gene="cusS"
                     /locus_tag="KLK57_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HBE0893104.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDAQGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTEPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVTVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
BASE COUNT         3473 a         3880 c         3559 g         3510 t
ORIGIN      
        1 caaaatggct gtttatttat acagtaaatc ctgtttcgga cttctcactc ctcatttaat
       61 caactgaagc tgagcccgtt ttttctgcat gcctcaatcg tcaaaattct tcgccatcag
      121 ccagctctgc cataacttgc cacattgaaa aagtagccaa attgcttcag tcaccatcat
      181 caaaaaaaga aacattccag aacttccgca tctcgcccag ccgctccgac ttctatactg
      241 aatagaaaac gccaacataa gagaaaccta tgccattacc cgattttcat gtttctgaac
      301 cttttaccct cggtattgaa ctggaaatgc aggtggttaa tccgccgggc tatgacttaa
      361 gccaggactc ttcaatgctg attgacgcgg ttaaaaataa gatcacggcc ggagaggtaa
      421 agcacgatat caccgaaagt atgctggagc tggcgacgga tgtttgccgt gatatcaacc
      481 aggctgccgg gcaattttca gcgatgcaga aagtcgtatt gcaggcagcc gcagaccatc
      541 atctggaaat ttgcggcggt ggcacacacc cgtttcagaa atggcagcgt caggaggtat
      601 gcgataacga gcgctatcaa cgaacgctgg aaaactttgg ctatctcatc cagcaggcga
      661 ccgtttttgg tcagcatgtc catgttggct gcgccagtgg cgatgacgcc atttatttgc
      721 tgcacggctt gtcacggttt gtgccgcact ttatcgccct ttccgccgcg tcgccatata
      781 tgcaaggaac ggatacgcgt tttgcctcct cacgaccgaa tattttttcc gcctttcccg
      841 ataatggccc gatgccgtgg gtcagtaact ggcaacaatt tgaagccctg tttcgctgtt
      901 tgagttacac cacgatgatc gacagcatta aagatctgca ctgggatatt cgccccagtc
      961 ctcattttgg cacggtggag gttcgggtga tggatacccc gttaaccctt agccacgcgg
     1021 taaatatggc gggattaatt caggctaccg cccactggtt actgacagaa cgcccgttca
     1081 aacatcagga gaaagattac ctgctgtata aattcaaccg tttccaggcc tgtcgctatg
     1141 ggctggaagg cgtcattacc gatccgcaca ctggcgatcg tcgaccgcta acggaagaca
     1201 ccttgcgatt gctggaaaaa atcgcccctt ccgcacataa aattggtgca tcgagcgcga
     1261 ttgaggccct gcatcgccag gtcgtcagcg gtctgaatga agcgcagctg atgcgcgatt
     1321 tcgtcgccga tggtggctcg ctgattgggc tggtgaaaaa gcattgtgag atctgggccg
     1381 atgactaagt agggaattgc cttacagaag aatatccctg acaatggtgt tttaacttac
     1441 atttaacaat gttatgaaac accctttaga aaccttgact accgcagcag gcattttgct
     1501 gatggctttc ctctcttgcc tgctgctgcc cgcccccgca ctggggctga cgctggcaca
     1561 aaaactggtg accatgttcc atctgatgga tcttagctag ctttacactt tattgttttg
     1621 tctgtggttt ttagtgctgg gcgctattga gtattttgtt ctgcgcttta tctggcgacg
     1681 ctggttctcg ctggcggatt aaacgtcgat tatcgccgta cttcgcggca tactttgctt
     1741 attctccttc gcctaaagga acgtttatgg ataagcaatc actgcacgaa acggcgaaac
     1801 gcctggccct tgagttaccc tttgtcgagc tttgctggcc ttttggcccg gagttcgatg
     1861 tttttaaaat tggcggcaaa atttttatgc tgtcgtcgga gctgcgcggc gtccccttta
     1921 tcaatctgaa gtccgatcca caaaaatccc tgttaaatca gcagatatac ccgagcatta
     1981 agccagggta tcacatgaat aaaaagcact ggatttcggt gtatcccggc gaggaaatct
     2041 ccgaagcgtt acttcgcgat ctgatcaacg attcgtggaa tctggtggtt gatggtctgg
     2101 ctaaacgcga tcaaaaaaga gtgcgtccag gctaaagcgg aaatctatag cgcatttttc
     2161 tcgcttacca tttctcgttg aaccttgtaa tctgctggca cgcaaaatta ctttcacatg
     2221 gagtctttat ggatatcatt tctgtcgcct taaagcgtca ttccactaag gcatttgatg
     2281 ccagtaaaaa acttaccccg gaacaggccg agcagatcaa aactctcctg caatacagcc
     2341 catccagcac caactcccag ccgtggcatt ttattgttgc cagcacggaa gaaggtaaag
     2401 cgcgtgttgc caaatccgct gccggtaatt atgtgttcaa cgaacgtaaa atacttgatg
     2461 cctcgcacgt cgtggtgttc tgtgcaaaaa ccgcgatgga cgatgcctgg ctgaagctgg
     2521 ttgttgacca ggaagatgct gatggccgct ttgccacgcc ggaagcgaaa gccgcgaacg
     2581 ataaaggtcg caagttcttc gctgatatgc accgtaaaga tctgcatgat gatgcagagt
     2641 ggatggcaaa acaggtttat ctcaacgtcg gtaacttcct gctcggcgtg gcggctctgg
     2701 gtctggacgc ggtacccatc gaaggttttg acgccgccat cctcgatgca gaatttggtc
     2761 tgaaagagaa aggctacacc agtctggtgg ttgttccggt aggtcatcac agcgttgaag
     2821 attttaacgc tacgctgccg aaatctcgtc tgccgcaaaa catcacctta accgaagtgt
     2881 aattctctct tgccgggcat ctgcccggct atttcccctc agattctcct gatttgcata
     2941 accctgtttc agccgtcatc ataggctgct gttgtataaa ggagacgtta tgcaggattt
     3001 aatatcccag gttgaagatt tagcgggtat tgagatcgat cacaccacct cgatggtgat
     3061 gattttcggt attatttttc tgaccgccgt cgtggtgcat attattttgc attgggtggt
     3121 actgcggacc ttcgaaaaac gagccatcgc cagttcacgg ctttggttgc aaatcataac
     3181 ccagaataaa ctcttccacc gtttagcttt taccctgcag gggattatcg tcaatattca
     3241 ggcggtattc tggctgcaaa aaggcaccga agcggcagat attctgacta cctgcgcgca
     3301 gttgtggatc atgatgtatg cgctgctttc agtcttctcg ttgctggatg ttattttgaa
     3361 tctggcgcag aaattcccgg cggcatctca gttaccgctg aaagggatat ttcaggggat
     3421 taaactgatc ggcgcgatcc tggtcggcat tttgatgatc tcgctgctga ttggtcagtc
     3481 gccagcgatt ctgatcagcg gtcttggtgc aatggctgcc gtgctgatgt tggtatttaa
     3541 agatccgatt cttggtctgg tggccggtat tcagctttcc gcgaacgata tgctgaaact
     3601 gggcgactgg ctggagatgc cgaaatacgg cgcagatggc gcggtgatcg atattgggtt
     3661 aaccaccgtc aaagtgcgta actgggacaa taccattacc actattccca cctggtctct
     3721 ggtttctgac tcctttaaaa actggagcgg gatgtcagca tctggcgggc gacgtattaa
     3781 gcgcagtatc agtattgatg tcaccagtat tcgttttctt gatgaagacg aaatgcaacg
     3841 tctgaataaa gcgcatttgt taaagcctta tttaaccagc cgccatcagg aaattaatga
     3901 gtggaatcgc cagcaaggtt ctacggagtc ggtattaaac ctgcgccgaa tgaccaatat
     3961 tggaaccttt cgtgcctatc tgaacgaata tctgcgtaac catccgcgga ttcgtaaaga
     4021 tatgacctta atggtacgcc aactggctcc aggtgataac ggtttaccgc tcgagatcta
     4081 cgcgtttact aacaccgtgg tgtggctgga atatgaaagc attcaggctg atatattcga
     4141 tcacatattt gcgattgtcg aagagtttgg tctgcgactt catcagtcgc caaccggcaa
     4201 tgatattcgc tctctggcgg gtgcatttaa gcagtaatta aaaaaaccgc tctcatcgaa
     4261 tggatgagag cggacatcgt tttgtcggat gtagcgtgaa tgccttattt ccgacgcagc
     4321 gttttaaatg ccacaaacag gaatacaatc cacaccggca gcaggatcgc tgacaagcgc
     4381 atatcatcca tcgtgcacat cagcagcaaa atcatgccga ggaaggcaat gcagagatag
     4441 tttccgaacg gatagagcag cgccttaaac tgtgtttcac gcccctgacg tcgcatcgct
     4501 gcacgaaaac gcagatgcgc cagacagatc ataatccagt tcaacagcag cgttgctacc
     4561 accagcgcca tcagcagacc aaacgctttt tgcggcagca gatagttgat taacaccacc
     4621 agcgaagtga tcgctccgga aagcatcagc gagttaatcg gcacaccgcg acggctgacg
     4681 cgagtcaaaa acttcggcgc attaccctgc acagaaaggc caaacagcat gcggctgtta
     4741 gagtaaaccc cgctgttata cactgacagc gatgctacca gaatgacgaa gttcagcgca
     4801 gaagctacca cgttgctgtc gagattatgg aaaatcatca caaacgggct actgttggat
     4861 ttcacttcca cccacggata gagcgccagt aaaaccacca gtgaaccgat gtaaaacagc
     4921 aggatgcgat acaccacctg atttaccgct tttggaatgc ttttatccgg atcgcgcgct
     4981 tcagcggcag taatcccaat cagctccaga ccgccgaagg agaacataat taccgccagc
     5041 gacaaaatca gcccattcca gccggtggcg aagaaaccac cgtagcgcca gaggttgtcg
     5101 atactggctt tctcgccgcc gtgaccagaa aacagcagcc acaggccaaa gccgatcata
     5161 ccgatgattg ccagcacttt aatcaacgca aaccagaact cggtttcgcc atataagcgc
     5221 acgttcacca ggttaacggc gttgatgata ataaagaagg cggcagccca aatccacgtt
     5281 ggaacatccg ggaaccagta ctgcatatag atgcccgcag cggtcagctc tgccattccc
     5341 accagcacga acattaccca gtagttccag ccagagagga agcccgcaaa cggtccccag
     5401 tatttatagg caaagtgggc aaatgaaccg gataccggct cttcgaccac catctcgcca
     5461 agctggcgca taatcaggaa agcgatgatc ccggcgacgc cgtaacccag caatacagcc
     5521 ggacccgcca tctgaatcgc cggaccaatg ccaagaaaca gaccagtacc aattgcgcca
     5581 cccagcgcaa tcagttgaat atgacggtta tgtaatccgc gatgaagcgt cggctcttga
     5641 ttcgacgcag tatcttccga tacggttgac gcgtttttca cgcctttccc ctgtgtgtct
     5701 tttttgttga ggggcacctt ttaacattta gtgcccatcg tcgcaagaca caatccacac
     5761 ggttaaacgg ggtatcctgc ttttatttcc gtacccgatg tcggtgcagc cacatcagct
     5821 tatacgccgc cgggataata aacagcgaca gcaaaggtgc ggtgatcatg ccgccaatca
     5881 tcggtgcggc aatccggctc atcacctctg aaccagcccc cgttccccac agaatcggca
     5941 gcagaccggc gataatcacc gccaccgtca tcgctttcgg gcgcacgcgc agtaccgcgc
     6001 cgtgatacaa cgcctcatcc agcttctgct cactgaatgt ttgcggatta ttcaacgacg
     6061 gctcggcctc tatggcgtga cgtaaataca tcagcatcac cacgccaaat tcggcggcga
     6121 ccccggcaag ggcaataaaa ccggtgcccg ttgccacaga aagatgaaag cccatccacc
     6181 acagcaacca gatgccgccc accagcgcaa acggtacgct gctgataatc agcaacgctt
     6241 cgcccacccg acggaatgcc agatacaaca gcacgaagat gatcatcagt gtcatcggca
     6301 ccatgagttt cagcttatgg ttggcgcgct ccagtagctc gaactgcccg gagaatgcca
     6361 cgctggtgcc cggttttaac tgcacttttt cagctatcgc tttttgcaaa tcgtgaacca
     6421 ccgacaccat gtcacgatcg cgggcatcaa tataaatcca gctcgtcggg cgcgcattct
     6481 cggttttcag catcgacggt ccggtagaga ctttaacgtc ggccacgtct gccagggtga
     6541 tttgctgctt catcggcgtc aggatcggta gctggcgcag tgcctgcggg ctatcgcgcc
     6601 agctttgcgg ataacgcaga ttaatgggat aacgggcaat cccttccacc gtttcgccaa
     6661 ccatcgcccc gcccaccgca gaagtcacaa acaactgcac atccgccacc gtcataccgt
     6721 aacgcgcggc tttttcacgg ttaatctcaa cgttgatata gcgcccacct tccagacgct
     6781 cggctagagc agaagccacg cttggcaccg ttcgcgctac ttcttcaatt tgctcagcca
     6841 tcgcgtcgat atccgccagc acagtgccgg aaactttaat gccgatgggg cttttaatgc
     6901 cggttgagag catatcgata cggttacgaa ttggcggcac ccacagattc gccagccccg
     6961 gcagccgcac ggtgttatcc agttcctcaa tgattttgtc catcgtcatg ccaggccgcc
     7021 actgatcctg cggcttaagc tggatggtcg tttcaaccat ctcgagcgga gcagaatcgg
     7081 tggcggtttc cgctttcccg gttttgccaa atacccgcgc cacttcaggt acgctcataa
     7141 ttagcttgtc ggttttttgc agcatactcg ccgcctctgc tgcggaaatc cccggcagcg
     7201 tcgatggcat atacaacaag tcgccttcat tgatctgcgg taaaaattcc ccgccaactt
     7261 tattgagcgg ccagagaacc gtcagcaccg aaagcgctgc caccaacagc gtggttttcg
     7321 gccagtgcag tactttcagc aatagaggat gataaacacg aatcaaaaag cgattgagcg
     7381 ggttactgct ttccggcgga attttgccac ggatccagta ccccatcagg atagggatca
     7441 ctacgatcgc cagcagcgcc gcgcccgcca tcgcataggt tttagtgaac gccaacgggc
     7501 caaacagacg cccttcctgc ccttccaggg tgaagatcgg gataaacgac aacgtgataa
     7561 tcagcagact gataaatagc gccggaccca cttcaacaga tgcatcggtg atcacctgcc
     7621 agcgcgtttt attatccagc gtggcgtcag gatgctggtg ctgccactct tccagccgtt
     7681 tatgtgcatt ttcgatcatg acgatagcgg catcgaccat cgccccgacg gcaatcgcaa
     7741 tgccgcccaa cgacataata ttggcattca gtccctggaa gtgcatgaca ataaaagcaa
     7801 tacacaaccc cagcggcaac gaaataatcg ccaccagcgc cgagcgcaca tgccagagaa
     7861 acagcgcaca gactaccgcc accacgataa actcttccag caacttgccg ctgaggttgt
     7921 cgatggcgcg gtcaatgagc tggctgcgat cgtatgttgt aactatctcc acgccttccg
     7981 gcagactact tttcagcgtt tccagtttgt ccttcacgga ggcgatcact tctcgggcgt
     8041 ttttgccgga gcgcaggatc accaccccgc ccgccacttc gccttcgcca ttcagttcgg
     8101 caatgccccg gcgcatctcc gggccaatct ggaccttcgc aacatcacgc agataaacgg
     8161 gcacgccatt ttctctggct tttaaaacga tgtgattaaa gtcgtcgagc gtttgcagat
     8221 agccgctggc gcgcaccata tattccgctt ccgccagttc gatcgacgaa ccgcccgctt
     8281 cctggtttga agcatccagc gcgcttttta cttcggctag actgatgcca tattgcgcca
     8341 ggcgctgggg atcgataacc acctgatact ctttcaccac gccgcccacc gacgccactt
     8401 ccgcaacgtc agggatggtt tttagctcat atttgagaaa ccagtcctgc aatgagcgta
     8461 aatcggccag atcgtgctta ccgctgcgat ccaccagtgc atattcatag atccagccaa
     8521 cacccgtggc atctggtccc agctcggcgc tgactcccgc aggcagctta ccctgtacct
     8581 ggttgaggta ctccagcacc cgcgaacgcg cccagtacgg atcggtacca tcttcgaaaa
     8641 tgacatacac ataagagtct ccaaactgcg agaaaccgcg cacagtcttc gcgccaggca
     8701 ccgacaacat ggtggtggtt agcggataag tcacctgatt ttcaacgatt tgcggtgcct
     8761 gaccgggata gctggtttta ataatcacct gcacatcgga gagatccggc agcgcatcca
     8821 ctggcgtgtt aatgatggtc taggtgcccc agatgctcag aaacaacgcg cccatcagca
     8881 ccaaaaaacg gttcgccacc gagcgacgaa taatccattc aatcattggt tattccctca
     8941 atgcgcattg gtagcacttt cagagcgcat ccgctccagt gcgccagaaa tattggcttc
     9001 ggaatcaatc aggaacaggc cgctggaaac caccttttca ccttccgcca gaccagagtg
     9061 taacgcggtg acgccttgcg atgcctggaa aacggcaacg cgtttcggta caaagcgtcc
     9121 gtcggcatca acggtaatca cccgctgttc gttgccggta tcaatcagcg cctgtgacgg
     9181 aatgagcagc atcggttcgc tggcggtgtt gagttgcaac caggcgttca tgcccggttt
     9241 tagcgcctcg tcggcgttgt cgacttccag acgcagctgc agcgtgcggg tcgcggcatc
     9301 cacgccaggt agcagcgtcc atttgcggat ggtgagtgtt ttatccggtc gcgccggaac
     9361 ggtgagcgta aactgcgagg catctttcac cagccaggcg atagactccg ggatcgcagc
     9421 agtgacccac accgggtcca taccctgaat tttcgctacc acgttatctt tggcgatatt
     9481 cattcccgcg cgcagatcaa acgcggtgat cacgccatca atgggtgctt tgagcgtaaa
     9541 gcgagtctgg attttttgcg tggcgatcag gcggcgaata tccgcctccg gcattcccgc
     9601 cagtcgcagc cgctcaagaa tgccttcagt ctgggtcgcc gtaccgccgg tttcgcgcag
     9661 cagtaaatac tcactctgcg cttccaccca gtcaggaatg gtcaggtcga gaagcggtgt
     9721 gcccttttgt actttatcgc ccacggtaag cggatacacc ttgtcgataa acccggcagc
     9781 gcgagcctgc acaatggcat actgatactc gttgtaactg acattcgccg ggaaactctg
     9841 ggcaaaagtc agcggtccgc gcgtgacggt agccgttttc acccccagat tctgcgtctg
     9901 agtcgggtca atgcgcacac cagacgcaga actctcttca tcggcatatt tcggcaccag
     9961 atccatatcc ataaacggcg atttacctgg tttatcgaac cgcgtattgg ggtacatcgg
    10021 gtcgtaccag aataagattt tacgttctgc ggtcgacgtt ttttctgcgg gtggttcagc
    10081 ctttgcaaac caggtaaaac ctgccgcaga aataataccg cccgcgatca tgctgccgat
    10141 aataagcgcg atttttttca tctcattaaa cctgggttac tggctgactt taatatcctg
    10201 taataaagaa aggttgccct gctggacaaa attaaacgcc actttgtcgc cggttttaat
    10261 ttcactcatt ttcgtctgcg gggtgatggt aaagcgcata gtcatctccg gccagttcac
    10321 ggcagcaatc ggatcgtgat ggatggtgat ttttttgctt tccagatcaa taccctttac
    10381 cacgccagtg gcgctaataa cctgcggttg tacttcgctc atggtttcat gatgatgttc
    10441 gttagcctgg gcattaaagc caataacggt aaacagactg aacattgcga cttgcagtgc
    10501 ttttttcatt tattctctcc tggagttaaa aattattgct gccaaccgcc ccctaaggcg
    10561 gtatacaaag aaatttcgtt aacctgacgg gcataattca ggtcgagtaa agtttgtcgg
    10621 gttgcaaata aagaacgctc ggcatccagc acttccagat aacttaccgc gccgtgctga
    10681 tataatgccc gcgcccgttg caaagtaatt tgcagcgacg ccagataacg ctgctgggcg
    10741 ctgatttgat cgttcaggct ttgacgtaat gccagcgcat ctgccacttc tttaaaggcg
    10801 ttctggattt tctgttcata attcactacc gactgctgct ggcgaatttc ggcgatatcc
    10861 agattggcct ggttgcgtcc ggcattaaaa atggggatct caattttagg aataaaattc
    10921 cacatcccgc tgctagcgtt aaataatgaa gacagatcgc tgctggcggt tgatattccg
    10981 ctggtcaggc tgatagacgg gaaaaatgcc gcacgcgcag caccaatatt agcattagcc
    11041 gccattaacg cgtgttcagc ttccataata tccgggcgct gcaataagat ttgcgacgac
    11101 aagcccgccg gtaatttaac gctttgcagg ctgccgctgt ttactgtctg cgcttgcggc
    11161 agtttgccgt agcttcccaa taacagttgc aatgcattat tcgcctgcgc cagttccccc
    11221 tgacgtttag cgatgtcact gcgggtactt tctatcaccc cgcgagcctg ttccagcgcc
    11281 agaacattgc tgctaccggt caacagttgt ttttcgacaa acgcatatga ctgctgataa
    11341 ttacgcagcg tttcttcggc tatttgcaat tgcgcatacg ccagttgctg attgaaatag
    11401 ctttgcgcga cattagaaac cagcagaata tgcaccgcgc gctgagcttc ctcagtggct
    11461 aaataatttt gtcgctcggc ttcgctcatg ttctttaagc gaccgaaaaa atcgaggtca
    11521 aagctggcgt taaggccggt cgaaaactcc cgtgtcgtgg cggtattccc tttaagatta
    11581 ccgctccagc tgccgctgcc ctcgccattg agctgtgggt agcggtcggc atcggtcaga
    11641 cgatattgcg cccgtgcctc ctgcactttc agcgtcgcca tgcgcaaatc ccggttattc
    11701 accagcgcct cgctaatcag cgtcttcacc tgattatcaa caaaaaaggt gcgccagccc
    11761 acgttctggt agttatctgc tgcgttaacc aagccgttct ggctgagtga gaactgctgc
    11821 ggcacgggca ttgccgtacg ctgataatcc ggtgccagtg aacaaccggt tagcgcaagg
    11881 gccacacaaa atggcagaag tttacaagga gacataggct cataatttct ggtgatttta
    11941 taccgccaac tttactcgcc aggctctgat tttccggtga caggaaaatg acaaaattgt
    12001 cattttgcca ataagcgatt gccatctgat cccgctactc tagaattgcc cgggcaacat
    12061 gcggaggaaa aatgaaactg ttgattgtcg aagatgaaaa gaaaaccgga gaatacttga
    12121 ccaaagggtt aaccgaagcc ggttttgtgg tcgatttggc cgacaacggg ctgaatggct
    12181 accatctggc gatgaccggt gattatgacc tgataatcct cgatattatg ctgccggacg
    12241 tgaacggctg ggatatcgtg cgcatgttgc gctccgccaa taaagggatg ccgattctgt
    12301 tgcttaccgc gcttggcacc attgaacatc gcgtcaaggg gctggagttg ggggccgatg
    12361 actatctggt gaagccgttc gcttttgctg aactgctggc gcgggtgcgc actctcctgc
    12421 ggcgcggggc ggcggtgatt atcgaaagtc agtttcaggt tgccgatctg atggtcgatc
    12481 tcgtcagccg caaagtcacc cgcagcggca cgcgcatcac tctgaccagt aaagagttta
    12541 ctctgctgga gttttttctc cgccatcagg gcgaagtgct gccccgctcg cttatcgcct
    12601 cgcaggtgtg ggacatgaat ttcgacagcg acactaacgc cattgatgtg gcggtgaagc
    12661 ggctacgcgg caaaatcgac aacgactttg agccgaagct gattcagacc gtgcgcggcg
    12721 tgggctatat gcttgaggtg ccggatggtc agtaagccat ttcagcgccc gttttcgctg
    12781 gcaacccgtc tgaccttttt tatcagcctg gccaccatcg cggcgttttt cgcctttgca
    12841 tggatcatga tccactcagt gaaagtgcat tttgccgagc aggatattaa tgatttaaaa
    12901 gaaattagcg ccacccttga acgggtactt aatcatcctg acgagacgca agcccgacgc
    12961 ttaatgacgc tggaagatat cgtcagtggt tattccaacg tgttgatttc cctggcagat
    13021 agccacggca aaacggtgta tcactccccc ggtgcgccgg atatccgcga gtttacgcgt
    13081 gacgccatac ccgataaaga cgctcagggt ggcgaggtgt atctcctttc cggcccgacg
    13141 attatgatgc caggccacgg tcacgggcat atggaacaca gcaactggcg gatgattaac
    13201 ttgccggttg gcccgttggt ggacggcaaa ccgatttata cgctctacat cgcgctttcg
    13261 atcgattttc atcttcatta cataaatgat ttgatgaata aacttattat gaccgcatct
    13321 gtcatcagca tcctgatcgt ctttatcgtg ctattggcgg tacataaagg tcacgcgccg
    13381 atccgcagcg tcagccgtca aatccagaat atcacctcga aagatctcga cgttcgcctt
    13441 gacccgcaga ccgagcccat tgagctggaa cagctggtac tgtcgttcaa ccatatgatc
    13501 gagcgtattg aggatgtctt tacccgccag tccaatttct cagcggatat cgctcacgaa
    13561 attcgcacgc cgattaccaa tctcattacg caaacggaaa tcgccctcag ccagtctcgc
    13621 agccagaagg agctggaaga tgtgctctac tctaatctcg aagagctgac gcgaatggcg
    13681 aaaatggtca gcgatatgct gtttctcgct caggccgata acaaccagct aatccccgaa
    13741 aagaaaatgc tcaacctggc ggatgaagtc ggcaaagtgt tcgatttttt cgaggcgtta
    13801 gcggaagatc gcggcgtgga gttgcgattt gttggcgaca aatgtcaggt tgcgggcgat
    13861 ccgctgatgc tgcgtcgggc gttaagcaac ctgctttcta atgccctgcg ttatacgcca
    13921 accagagaga caattgtagt gcgctgtcag acggtcgatc accaggtgca agttaccgtc
    13981 gaaaaccccg gtacgcccat tgcgcccgag cacttaccgc gattgtttga ccgtttctac
    14041 cgcgttgacc cttcccgcca gcgaaaaggc gaaggtagcg gcattgggct ggcgatagtg
    14101 aaatcgattg ttgtcgcgca taaaggcacg gttgcagtaa cgtcagatgc gcgggggaca
    14161 aggtttgtga ttatgctgcc ggagagagag tgattattaa gttcaaattc agggcgtggt
    14221 gcatcacctc ctggtattgt tgatcaacaa attagagaaa aacttattaa acatatgaaa
    14281 tatcgaaggc ttttattttt caaagtttca cgagatgttt gtatatgaaa cttagttaaa
    14341 tctgtgtttc ttccaattgt agatcacaat gccctttcta aattttcatc gttataaaca
    14401 agccatctat catataaagc aa
//
LOCUS       DAEEKQ010000099        14307 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.099, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000099 DAEEKQ010000000
VERSION     DAEEKQ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14307
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.099"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            20..1246
                     /gene="rtcB"
                     /locus_tag="KLK57_002783"
     CDS             20..1246
                     /gene="rtcB"
                     /locus_tag="KLK57_002783"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HBE0893105.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVFARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            1250..2266
                     /gene="rtcA"
                     /locus_tag="KLK57_002784"
     CDS             1250..2266
                     /gene="rtcA"
                     /locus_tag="KLK57_002784"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HBE0893106.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRLGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAVVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(2309..5014)
                     /gene="malT"
                     /locus_tag="KLK57_002785"
     CDS             complement(2309..5014)
                     /gene="malT"
                     /locus_tag="KLK57_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HBE0893107.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSWICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKTFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIHTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            5625..8018
                     /gene="malP"
                     /locus_tag="KLK57_002786"
     CDS             5625..8018
                     /gene="malP"
                     /locus_tag="KLK57_002786"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HBE0893108.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            8028..10112
                     /gene="malQ"
                     /locus_tag="KLK57_002787"
     CDS             8028..10112
                     /gene="malQ"
                     /locus_tag="KLK57_002787"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HBE0893109.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVKA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVADQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(10157..11473)
                     /gene="gntT"
                     /locus_tag="KLK57_002788"
     CDS             complement(10157..11473)
                     /gene="gntT"
                     /locus_tag="KLK57_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBE0893110.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIDSIKVGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKRIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(11834..12409)
                     /gene="nfuA"
                     /locus_tag="KLK57_002789"
     CDS             complement(11834..12409)
                     /gene="nfuA"
                     /locus_tag="KLK57_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HBE0893111.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(12468..13151)
                     /gene="gntX"
                     /locus_tag="KLK57_002790"
     CDS             complement(12468..13151)
                     /gene="gntX"
                     /locus_tag="KLK57_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HBE0893112.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSLLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            13189..13959
                     /gene="bioH"
                     /locus_tag="KLK57_002791"
     CDS             13189..13959
                     /gene="bioH"
                     /locus_tag="KLK57_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HBE0893113.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSQGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDDWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVPMPFLRLYGYLDGLVPRKVVPML
                     DKLWPRSKSYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(13988..>14307)
                     /locus_tag="KLK57_002792"
     CDS             complement(13988..>14307)
                     /locus_tag="KLK57_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HBE0893114.1"
                     /translation="LMGLVEQMACLLSSGYANDRQIKGLFNYILQTGDAVRFNDFIDG
                     VAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADIMRFTGVSEEELAA
                     ASQ"
BASE COUNT         3443 a         3568 c         3996 g         3300 t
ORIGIN      
        1 acgaaaagag aaaaacaaaa tgaattacga attactgacc actgaaaatg ccccggtaaa
       61 aatgtggacc aaaggcgtgc cggtagaagc cgatgcgcgt cagcaactta ttaatacggc
      121 gaagatgccg tttattttca aacatattgc ggtaatgcct gatgtacacc tgggtaaagg
      181 ttccaccatt ggtagcgtga tcccgaccaa aggggcgatt attccggcgg cggtgggcgt
      241 ggatattggc tgtggaatga acgcgctgcg taccgcgtta acggcggcag acctgcctga
      301 aaacctggcg gagctgcgtc aagcgattga aacggccgta ccacacgggc gtactaccgg
      361 gcgttgtaaa cgtgataaag gcgcatggga aaacccgcct tttaacgtcg atgcgaaatg
      421 ggctgagctg gaggccggtt atcagtggtt aacgcaaaaa tatccgcgtt tccttaacac
      481 taacaactat aaacacctgg gaacgctggg aacgggtaac cactttattg aaatctgcct
      541 tgatgagtcg gagcaggtgt ggattatgct gcactccggt tcacgcggaa ttggtaacgc
      601 catcgggact tactttatcg atctggcgca aaaagagatg caggaccaac ttgagacgtt
      661 gccgtcgcgt gatctggcgt actttatgga aggtacggaa tactttgatg attacctaag
      721 agccgtggcc tgggcgcagc ttttcgccag ccttaaccgc gatgcgatga tggaaaacgt
      781 ggtaacggca ttgcagagca ttacgcagaa aacggtcaga cagccacaaa cgctggcgat
      841 ggaagagatc aactgtcacc acaactatgt gcaaaaagaa cagcactttg gtgaagagat
      901 ctacgtgacg cgtaaaggcg cggtgtttgc gcgtgctggt caatatggaa ttattccagg
      961 gtcgatggga gcaaaaagct ttatcgtccg tgggctggga aatgaagaat ctttctgttc
     1021 gtgcagccac ggtgccgggc gggtaatgag ccgtactaaa gcgaaaaaac tgttcagcgt
     1081 ggaagatcaa attcgtgcca ccgcgcatgt ggaatgccgt aaagatgccg aagtgatcga
     1141 cgaaatcccg atggcgtata aagatattga tgcggtgatg gcggcacaaa gcgatctggt
     1201 ggaagttatc tataccctgc gtcaggtggt gtgcgtaaaa ggataaatga tgaaaaggat
     1261 gattgcgctg gatggcgcac agggcgaagg tggcgggcag atcctgcgct cggcgctgag
     1321 cctgtcgatg ataaccggcc agccatttac catcaccagc attcgtgccg ggcgggcgaa
     1381 accggggctg ttgcgccagc atctgaccgc ggtaaaagcg gctgcggaaa tttgtagggc
     1441 aacggtggaa ggcgcggagc tggggtcgca gcgtctggtc ttccggcttg gcaccgtgcg
     1501 cggcggcgag taccgctttg ctatcggtag tgccggaagc tgtacgctgg tgctgcaaac
     1561 ggtgctgccc gcgctgtggt ttgccgatgg accttcgcgt gttgaagtga gcggaggcac
     1621 cgataacccg tcggccccgc ctgcggattt tatccgccgg gtgctggagc cgctgctggc
     1681 gaaaatagga attcatcagc aaaccacgct gttacgtcac ggtttttatc ctgccggagg
     1741 cggcgtggtg gcaacggaag tctcgcccgt ggcatcgttt aacaccttgc aacttggcga
     1801 gcgcgggaac attgtgcaga tgcgtggaga agttctatta gctggtgtgc cgcgccatgt
     1861 tgctgagcgt gaaatcgcta cactggcggg gagtttttcc ctgcatgaac agaatattca
     1921 taacctgccg cgcgaccagg ggccgggtaa taccgtctcg ctggaagtcg aaagtgaaaa
     1981 tatcactgaa cgctttttcg tcgtcggtga aaagcgcgtc agtgccgagg tggtagccgc
     2041 gcagttagtg aaagaggtga aacgctacct ggcaagcccg gcggtagtgg gggaatatct
     2101 cgccgaccag ctggtgctac cgatggcgct ggcgggcgcg ggagaattta cggtcgccca
     2161 tccttcatgc catctgctga ccaatatcgc cgtggtggag cgtttcttgc cggtgcggtt
     2221 tggtttgata gaaacagatg gcgtaacgcg ggtgagtatt gaatgatgta aagatgtaaa
     2281 ccggatccaa tgcattatcc ggctaaactc acaccccata tcccatcatc ttcagcagtt
     2341 tctgcgcgtg ttgtaccgcg gcctgacggt gggcgacgcc gagtttttgg tacagattgc
     2401 ggatatgcgt cttgatggtg gttgccgcca cttccagctc gcccgcaatt tgctcattgc
     2461 tgtaaccgga gtagatcagc cccaggacct gccattcacg ttgggtcaac gggctggtgt
     2521 ggatcagttc tggcacttcc ggatgattca gcagacgttc aacgaaattc tcatcgaaat
     2581 gggcgaattt atgccgatga tgttgattga tttctcgcag aatacgctgc gcgcgatgct
     2641 gttccagttc cggcagcgta ttgagttgaa tcagctggcg cagttgttgc gccatcgctt
     2701 cgccttcgat gacaaaatga ctgataaatc cggtgcgatt cgccagtttt aatgcgtcca
     2761 gcaacacgcg ctgggcgtca cttttacgtc cggcctgcca gtacagttga ttaagcagca
     2821 acaggttacg gttgagatcg ctcatcaacc gcagactgcg ggcattttca ttgagttctt
     2881 cgagaacaat ttccgcaggt tcaaactcgc ccagcaagat ttgtgcgcgg gcaatgttgc
     2941 gccattgacc ttgcaggaag tggttgttcg caaactccgg tttagccgta tgacgcaacc
     3001 agttggcagc ggcggcttta tcgccggtca tttgccagta aatcacccgg actttgttgg
     3061 cgttagagat ccagtcgctg tgatatttgc cattccccag caggttttcc agacggttca
     3121 gctggctacg ggcgttatct aaatcaccac gggccagcga gcattgaatc aacattgcca
     3181 ggcactgaag ctgttgctgt ggctgataag acgacaagac ttcaatcccg ctacgcgccg
     3241 acgcttcggc ttcatccagc cgtgcccagg cccataacag ttgtgctcgg atgcgcacca
     3301 gaaactcatg catcggcagc tgttccagat gctgctcgtt gatcagctgg aacgtttttt
     3361 cctgtgtttc ccacgcggtt tgcaggaacc cttgggcaaa cagaatttca ctttgctgga
     3421 ttaaactcca taaggcgtag tgccagacgt cgtgctggcg tgccatctgt tcggtttgct
     3481 gcattagcgc cagtgagcgg gtcaattcgc ctttgcagtg cagcacttca cccagcaccg
     3541 aggttgctac aatgcggcta tagaaccagc ccggtggcag ctcttccagt gccagttttg
     3601 ccagccgttc cgcttcatcc ggattaccat cgttaatcgc cacctgggcg cgcagagcgt
     3661 taaattctgc gtgcatggtg ccttctctga tgtccttgat ttcatgttca gcacgggcta
     3721 gcagggtgtt aacttcgcca tagcgatgtt ggctttgcat tagccacgcc tgcaataaca
     3781 ctaactgcgg attttccagc aaactgtccc acggcagggc cttaagcgac tcttccagca
     3841 gcgacagttc gctatggttg aacaaactcc aggcgtgatt aagcagaata tcgcgcagca
     3901 tcagcgcatc gcctgccgcc agcgcatgat gaatcgcttc gctgggaaat ccctgggcca
     3961 tccagctttc tgcggcggca cggtggattt ccggcagctc cgccgccagt tcccactggc
     4021 agcgctggcg caggaagttg ccaaacagcg ggtgatagca gaaccactcg ccggtatcat
     4081 ccatccgctg taaaaacagc ccctgacgct caatctcttc gagacgcatt tgcccgtttt
     4141 cttcgccagt cacacgggtg atgagggcat cgttcattga gcgcaaaatg gcgcttttca
     4201 acagaaaatg gcgcgttgcg agatcgacgt tatccaaaac ctcatcgacc agataatccg
     4261 aaagatggct ggcattgatt cccgccaggc ggcgtgccga cttatgggct gagtgggtat
     4321 tctgccgggc ggagagggcg attagctgta gtgccgtcgc ccaaccggaa acgtcatcgc
     4381 aaatccaact gctttctgcg gcttcaatcg gcgatgacag acggcaatca aaaaactgct
     4441 tcgcttcctg atgggtaaat gccagttgct gactgccaat ttccagcagt tggtcacgaa
     4501 cacgcagatt ggcaatgccc agttgcggaa ggttgcgtga caacaccacc agggtgagat
     4561 tttctggttg atggcgaata aagaagcgca ttgactcgtg gatcactgga ttagtgatca
     4621 gatgatagtc atcgatgacc agataaagtg ggctatgcca ttccgccagc tcaatgaaaa
     4681 gctgggcgaa gagtgacgtc aggctggcat attgccgttt ttgcgccatc gtctcacata
     4741 tcgcacagtg accgttggtt gcctgctgca cggcggcaat gagatagctg gcgaaacgct
     4801 cttgctggtt atcaccttca tccagcgagt accagccgat atcgtttttg cctgccgccc
     4861 actgggaaat gagggtggtc tttccgtagc ccgcaggact cgtgatcagc gccagccgga
     4921 agttgttcgc gccggaaagt ttagccagca ggcgctcacg aaccacggta tggtcgagtc
     4981 gaaccggacg acttagtttt gacggaatca gcatagttaa tcacttcact gtggaaaatg
     5041 aggaaatatt atttttttgc gcttcgtaat taatggttat aaggtcggcc agaaaccttt
     5101 ctaatgcaag cgatgacgtt tttttatgtg tctgaatttg cactgtgtca caattccaaa
     5161 tctttattaa caactcacct aaaacgacgc tgatccagcg tgaatactgg tttcccttat
     5221 gttcatcaga ttcatttaag caagggtttc ttcttcattc ctgatgaaag tgccatctaa
     5281 aaagatgatc ttaataaatc tattaagaat gagatggagc acattggata ttttacttgt
     5341 gaaactgttt cactccttta cttaatttat agagttacct tccgcttttt gaaaatacgc
     5401 aacggccatt ttttgcactt agatacagat tttctgcgct gtattgcatt gatttgctgc
     5461 taatcctgtg gtttgcacta gctttaagtg gttgagatca catttccttg ctcatccccg
     5521 caactcctcc ctgcctaatc ccccgcagga tgaggaaggt caacatcgag cctggcaaac
     5581 tagcgataac gttgtgttga aaatctaaga aaagtggaac tcctatgtca caacctattt
     5641 ttaacgataa gcaatttcag gaagcgcttt cacgtcagtg gcagcgttat ggcttaaatt
     5701 ctgcggctga aatgactcct cgccagtggt ggctagcagt gagtgaagca ctggccgaaa
     5761 tgctgcgtgc tcagccattc gccaagccgg tggcgaatca gcgacatgtt aactacatct
     5821 caatggagtt tttgattggt cgcctgacgg gcaacaacct gttgaatctc ggctggtatc
     5881 aggatgtaca ggattcgttg aaggcttatg acatcaatct gacggacctg ctggaagaag
     5941 agatcgaccc ggcgctgggt aacggtggtc tgggacgtct ggcggcgtgc ttcctcgact
     6001 caatggcaac tgtcggtcag tctgcgacgg gttacggtct gaactatcaa tatggtttgt
     6061 tccgccagtc ttttgtcgat ggcaaacagg ttgaagcgcc ggatgactgg catcgcagta
     6121 actacccgtg gttccgccac aacgaagcac tggatgtgca ggtagggatt ggcggtaaag
     6181 tgacgaaaga cggacgctgg gagccggagt ttaccattac cggtcaagcg tgggatctcc
     6241 ccgttgtcgg ctatcgtaat ggcgtggcgc agccgctgcg tctgtggcag gcgacgcacg
     6301 cgcatccgtt tgatctgact aaatttaacg acggtgattt cctgcgtgcc gaacagcagg
     6361 gcatcaatgc ggaaaaactg accaaagttc tctatccaaa cgacaaccat actgccggta
     6421 aaaagctgcg cttgatgcag cagtacttcc agtgtgcctg ttcggtagcg gatattttgc
     6481 gtcgccatca tctggcgggg cgtaaactgc acgaactggc ggattacgaa gttattcagc
     6541 tgaacgatac ccacccaact atcgcgattc cagaactgct gcgcgtgctg atcgatgagc
     6601 accagatgag ctgggatgac gcttgggcca ttaccagcaa aactttcgct tacaccaacc
     6661 ataccctgat gccagaagcg ctggaacgct gggatgtgaa actggtgaaa ggcttactgc
     6721 cgcgccatat gcagattatt aacgaaatta atactcgctt taaaacgctg gtggagaaaa
     6781 cctggccggg cgatgaaaaa gtgtgggcca aactggcggt ggtgcacgat aaacaagtgc
     6841 atatggcgaa cctgtgtgtg gttggcggtt tcgcggtgaa cggtgttgcg gcgctgcact
     6901 cggatctggt ggtgaaagat ctgttcccgg aatatcacca gctatggccg aacaaattcc
     6961 ataacgtcac caacggtatt accccacgtc gctggatcaa acagtgcaac cctgcactgg
     7021 cggctctgtt ggataaatca ctgcaaaaag agtgggctaa cgatctcgat cagctgatca
     7081 atctggaaaa attcgctgat gatgcgaaat tccgtcagca atatcgcgag atcaagcagg
     7141 cgaataaagt ccgtctggcg gagtttgtga aagttcgtac cggtattgag atcaatccac
     7201 aggcgatttt cgatattcag atcaaacgtc tgcatgagta caaacgccag cacctgaatc
     7261 tgctgcatat tctggcgttg tacaaagaaa ttcgtgaaaa cccgcaggct gatcgcgtac
     7321 cgcgcgtctt cctcttcggc gcgaaagcgg caccgggcta ctacctggcg aagaatatta
     7381 tctttgcgat caacaaagtg gctgacgtga tcaacaacga tccactggtt ggcgataagt
     7441 tgaaggtggt gttcctgccg gattattgcg tttcggcggc ggaaaaactg atcccggcgg
     7501 cggatatctc cgaacaaatt tcgactgcag gtaaagaagc ttccggtacc ggcaatatga
     7561 aactggcgct caatggtgcg cttactgtcg gtacgctgga tggtgcgaac gttgaaatcg
     7621 ccgagaaagt cggtgaagaa aatatcttta tttttggtca taccgtggaa caagtgaagg
     7681 caattctggc caaaggctac gacccggtga aatggcggaa gaaagataag gtgctggacg
     7741 cagtattgaa agagctggaa agcggtaaat acagcgacgg cgataagcat gccttcgacc
     7801 agatgctgca cagtatcggc aaacaaggcg gcgatccgta tctggtgatg gcggatttcg
     7861 cagcctatgt tgaggcacaa aagcaggtgg atgtgctgta ccgcgaccag gaggcctgga
     7921 ctcgcgcggc gatcctcaat accgcccgct gcggtatgtt tagctcggat cgctctattc
     7981 gcgattatca ggctcgtatc tggcaggcaa aacgctaagg aagctcgatg gaaagcaaac
     8041 gtctggataa tgccgcgctg gcggcgggga ttagccccaa ttacatcaat gcccacggta
     8101 aaccgcagtc gattagcgcc gaaaccaaac ggcgtttgct tgacgcgatg catcaacgta
     8161 ccgccacgaa agtggcggta acgccagtcc cgaatgtcat ggtttatacc agcggcaaaa
     8221 aaatgccgat ggtggtggag ggcagcggcg aatatagctg gctgctgacc accgaagaag
     8281 gaacacagta caaaggccat gtaacggggg gcaaagcgtt caatctaccg acgaagctgc
     8341 cggaaggtta tcacacgctg acactcaccc aggacgacca gcgcgcgcat tgccgggtga
     8401 ttgtcgcccc gaaacgctgt tacgaaccgc aggcgttgct gaataaacaa aagctgtggg
     8461 gtgcctgcgt tcagctttat acgctgcgat cggaaaaaaa ctggggtatt ggggattttg
     8521 gcgatctcaa agcgatgctg gtggatgtgg caaaacgtgg cgggtcgttc atcggcctga
     8581 acccgattca tgcgctctat ccggcaaatc cggagagtgc cagcccatac agcccgtctt
     8641 ctcgccgttg gctgaatgtg atttatatcg acgttaaagc cgtcgaagat ttccatctta
     8701 gcgaagaggc tcaggcctgg tggcagttgc cgaccacgca acagacgctg caacaagcgc
     8761 gggatgccga ctgggtcgat tactccacgg ttaccgccct aaaaatgaca gcattacgaa
     8821 tggcgtggaa aggtttcgcg caacgtgatg atgagcagat ggccgcgttt cgccagtttg
     8881 ttgcagatca aggcgacagc ctgttctggc aggcagcctt tgatgcgcta catgcccagc
     8941 aagtgaaaga ggacgaaatg cgctggggct ggcctgcatg gccagagatg tatcagaacg
     9001 tggattcacc agaagtgcgt cagttctgcg aagaacatcg taatgacgtc gatttttatc
     9061 tctggttgca gtggctggct tacagccagt ttgccgcctg ctgggagata agccagggct
     9121 atgaaatgcc gattggcttg tatcgtgatc tggcggttgg cgtagcggaa ggtggggcgg
     9181 aaacctggtg tgaccgtgaa ctatattgcc tgaaagcgtc ggttggcgcg ccgccggata
     9241 tcctcggccc gttggggcag aactggggat taccgccaat ggacccgcat atcatcaccg
     9301 cgcgtgccta tgaaccgttt atcgagctgt tgcgtgccaa tatgcaaaac tgcggcgcat
     9361 tacgaattga ccatgtgatg tcgatgctgc gtttgtggtg gataccgtat ggcgagacgg
     9421 cagatcaggg cgcgtatgtt cactatccgg tggatgatct gctctcgatt ctggcactcg
     9481 aaagtaaacg tcatcgctgt atggtgattg gtgaagatct cggtaccgta ccggtagaga
     9541 ttgtcggtaa gctgcgcagc agcggtgtgt actcctacaa agtgctctat ttcgaaaacg
     9601 accacgagaa gacgttccgt gcaccgaaag cgtatccgga gcagtcgatg gcggttgcgg
     9661 cgacacatga cctgcctacg ctacgcggtt actgggagag cggggatcta acgctgggca
     9721 aaaccctggg gctgtatccg gatgaagtgg tactgcgcgg tctgtatcag gatcgcgaac
     9781 tggcgaagca agggctgctg gatgcactgc ataaatatgg ttgtctgcca aaacgtgccg
     9841 ggcataaggc atcgttgatg tcgatgacgc cgacgctgaa ccgtggtttg cagcgctaca
     9901 ttgccgacag taacagtgct ctgttaggac tacagccgga agactggctg gatatggccg
     9961 aaccggtgaa tattcctggc accagttacc agtataaaaa ctggcgacgc aagctttccg
    10021 caacgcttga gtcgatgttt gccgatgatg gcgtgaacaa gttgctgaag gatttggaca
    10081 gacggcgcag agctgcagcg aagaagaagt agagtgcggt tgatgccgga tgcgatgcat
    10141 ccggcagtca gtgttatcaa atcaccatat tcagcagcag acagcctacc agaccgcaca
    10201 ccgagataat ggtttccagc atcgaccagg atttgatggt ctcgccgata gtcaggttaa
    10261 agtactcttt gaacagccag aagcccggat cgttcacatg agagaaaatc acactaccgg
    10321 aacccaccgc aataaccatc agctctgggc taacacccgt cgttgcaatc agcggtgccg
    10381 cgataccacc cgcagtgatt gccgcaacgg ttgcggaacc cagcgcgata cgcagtacgg
    10441 cagcaatcga ccaggccatc agcagcggag aaatgttggt ttcgtgcatc atggaagcaa
    10501 tgtatttgtc cacgccgctg tctaccagca cctgcttgaa cgcaccgcca ccaccgatga
    10561 tcaacagcat catcgcaatg attttgatgg aagaaaccag cgtgtcgtta atctgatcca
    10621 ttgaacgacc acggttcaga ccaaaggtga acatcgcaat cagcacggca atcagcgttg
    10681 ccattaccgg gtcaccgagg aactccgcta ccggcaggaa agcgtgacct ttcggcagga
    10741 tcatttcggc aatcgcacgc atcgccatca gcacaaccgg caccagagaa gtccagacgc
    10801 tgacgccaaa gctcggcatc tcttcttcgc tgaaggtttt cgcgctgtag agaccttccg
    10861 ggattggctt atcgatacct ttcaacacgc gagcgtaaac cggaccggcg agaatcacgg
    10921 tcgggattgc cagaatagta ccgtacagca gggttttacc catatcggca ttgaaaatgg
    10981 tggcaatcgc agtcgggccc ggatgcggtg gcaggaagcc gtgggtcaca gacagtgcag
    11041 ccgccattgg tacaccaaca tacagcagtg gaatattcgc ggaagccgcg atggtaaaca
    11101 ccagcggcag catcagcacg aagcccactt catagaacag ggcaaaacca acggtaaaac
    11161 cggttaacac caccgcccac tggatgcgct ttttaccaaa tttggcaatc agcgtggtgg
    11221 cgatacgttg tgcgccaccg cagtctgcca gcattttgcc cagcattgcg ccaaaaccca
    11281 tgatcagggc aaggctaccg agcgtcccgc cgacaccgac tttgatggag tcaataactt
    11341 tatccagcgg cattccttgc attaatccaa cagcaagcgc cacgaggacg agagcgatga
    11401 agccgttcat tttgaagcgg atcatcagga gcaacaacaa gattacaccg atagcaacaa
    11461 tgactaatgg catgatttac ctggcctttc atttgttatg ggtaacgtca attttctgac
    11521 gacaaactct aattatccca atcgggaaca gagatattgc ggcaccacga ctgataccca
    11581 ctaaaactaa ttattgtagt cagatgtcag gagtatgttt ggtacccatg tgaatgatac
    11641 gggtaacatt tggcgtttga gaatcaccag agcggggtaa atttaaatta tgagaggttg
    11701 gtcatattat cgcggggaaa cgaagggagg gatttgccaa agcaatgctg tgccaacgtc
    11761 tggcacatgt tcaacgtagg cccgaaatga cgctttagcg tcgcatcggg caatctacaa
    11821 aagaggggat aacttagtag taggagtgtt cgccgcgctg gtgttcggtg agatcgcgca
    11881 cacctttcag ctccgggaat tcgttcagca gctgcttctc gatcccttct ttcagcgtca
    11941 catcgaccat ggaacaaccg ttacagccgc cgccaaattg cagaatggcg taaccgtctt
    12001 cggtgatttc catcagcgaa acgcgaccac cgtgaccagc aagctgtggg ttgatctgcg
    12061 actgcagcat atactccacg cgctccatca gcggtgcatc gtctgccact ttacgcattt
    12121 tggcgttcgg ggctttcagc gttaactgag aacccaactg gtcggtaaca aagtcgatct
    12181 ctgcatcttc caggtatggt gcgcttaact catcaacata cgcggtcagc aggtcaaatt
    12241 tcagggcagt gtcggtggct tccacggcat ccggcggaca ataagaaaca ccacattcag
    12301 cgttaggcgt gccagggtta atcacaaata cgcggatttg tgtcccttct tcctgatttg
    12361 ccagcagttt ggcaaagtgc gcttgtgcag catcggaaat acggatcata gtaatggcct
    12421 aatagttgac tattttagtt ggttataata cgcccatcat cgaggctcta caaggttcga
    12481 caaaggcacc agacctggac agtcgccgca ccattgcgta aaagcaactg cgcaatctct
    12541 gcgacggtac ttccggtggt aacgacatca tccacaatca ccatatggcg accttgcacg
    12601 ggcaattcaa gacgaaaggc atttttcagg ttgcgcttgc gcagccgggc actgagaaaa
    12661 tgctgggtcg cagtggcccg tgtacgtgtg acggcttcgc tatcccattg gcagtgcaac
    12721 cagcgtgata acggctgaca cagcaaatcg ctctgattaa atccccgacg ccagtgacgc
    12781 cgctgccata acggaacgct gatgatgcga tccggcaatt gcaacccggt ggtgcgacga
    12841 gcgtgtaaga cttccaatag taacagacgt gacagggcgc tggcgatttc actgcgccgg
    12901 gaaaatttaa gctggtggat gagcagactt aacggcggcg catagtcggc aaccgtgacc
    12961 agtctttgcc agggcggcgg tttttgcagg cagcgaccgc agggaagatg ggagtgtgtg
    13021 gcgggtaatc cacattgtgg gcataacgtt ttatctgtgc gggtggcgcg tgaacagacc
    13081 gaacaaatcc cccaatgacc taacgccagt ggcattcggc atagccagca taatcccggt
    13141 actgttagca tatgttcatc cttgtaagtc aaaagagaac aatagcggat gaataacatt
    13201 tggtggcaga ccaaaggtca ggggaatgtt catcttgtgc tgctgcacgg atggggactg
    13261 aatgccgaag tgtggcgttg cattgacgag gaacttagct cgcattttac gctgcacctt
    13321 gttgatctgc ccggcttcgg gcgcagccag ggatttggtg cgctgtcact tgctgatatg
    13381 gccgaagccg tgctgcaaca ggcacctgat aaagccattt ggttaggctg gagtctgggc
    13441 gggctggtgg caagccagat tgcgttaacc catcccgagc gtgttcaggc gctggtcacc
    13501 gtggcgtcgt caccttgttt tagtgctcgt gacgattggc cggggataaa accggacgtg
    13561 ctggcgggat ttcagcagca actcagtgat gattttcagc gtacagtgga gcggttcctg
    13621 gcgttacaaa ccatggggac tgaaacggcg cgccaggatg cgcgggcgtt gaagaaaacc
    13681 gttctggcgt taccgatgcc ggaggttgac gtgcttaatg gcgggctgga aatcctgaaa
    13741 acggttgatc tccgtcagcc gctgcaaaac gtgcccatgc cgtttttgcg attgtatggc
    13801 tatctcgatg gtctggtgcc gcgcaaagtg gtgccgatgc tggataaact ctggcctcgc
    13861 agcaaatcat atatcttcgc caaagcggcc catgcgccgt ttatttcgca tccggtcgag
    13921 ttttgtcacc tgctggtggc gttgaagcag agggtgtagg caacttttga aatggcgaga
    13981 cagaaattta ctggctcgcc gcagccaact cttcttctga caccccggta aagcgcatga
    14041 tgtccgcaag agggactccg gattcaagca ttattttggc tatatgcagg gctttggatt
    14101 gttccccctc ctgccgcaat ctttccgcaa ttgtcattaa actctccttg tgtttcggtg
    14161 aacgttcggc aacgccgtcg ataaaatcgt taaaacgtac cgcgtcgccg gtttgcagta
    14221 tgtaattaaa cagccctttg atttgtctgt cattagcgta tccactactt aataagcagg
    14281 ccatttgctc taccagcccc atcaggt
//
LOCUS       DAEEKQ010000100        14107 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.100, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000100 DAEEKQ010000000
VERSION     DAEEKQ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.100"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(13..1371)
                     /gene="hprS"
                     /locus_tag="KLK57_002793"
     CDS             complement(13..1371)
                     /gene="hprS"
                     /locus_tag="KLK57_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HBE0893115.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGYLNIDVASPGTK
                     IHEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRIMLPQ
                     RN"
     gene            complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="KLK57_002794"
     CDS             complement(1371..2042)
                     /gene="hprR"
                     /locus_tag="KLK57_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HBE0893116.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRNNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            2175..2588
                     /gene="hiuH"
                     /locus_tag="KLK57_002795"
     CDS             2175..2588
                     /gene="hiuH"
                     /locus_tag="KLK57_002795"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HBE0893117.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2697..3701
                     /gene="msrP"
                     /locus_tag="KLK57_002796"
     CDS             2697..3701
                     /gene="msrP"
                     /locus_tag="KLK57_002796"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HBE0893118.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPTAWQNNLPLTPVDKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            3702..4337
                     /gene="msrQ"
                     /locus_tag="KLK57_002797"
     CDS             3702..4337
                     /gene="msrQ"
                     /locus_tag="KLK57_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HBE0893119.1"
                     /translation="MRLTAKQVTWLKVSLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFKRLRKQVHNKLSV"
     gene            4594..5244
                     /gene="zinT"
                     /locus_tag="KLK57_002798"
     CDS             4594..5244
                     /gene="zinT"
                     /locus_tag="KLK57_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HBE0893120.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKIKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQFLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            5587..6117
                     /locus_tag="KLK57_002799"
                     /pseudo
     CDS             5587..6117
                     /locus_tag="KLK57_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
     gene            complement(6687..6776)
                     /locus_tag="KLK57_002800"
     tRNA            complement(6687..6776)
                     /locus_tag="KLK57_002800"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6740..6742),aa:Ser,seq:cga)
     gene            6870..7667
                     /gene="mtfA"
                     /locus_tag="KLK57_002801"
     CDS             6870..7667
                     /gene="mtfA"
                     /locus_tag="KLK57_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HBE0893121.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTGLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            7768..7843
                     /locus_tag="KLK57_002802"
     tRNA            7768..7843
                     /locus_tag="KLK57_002802"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7801..7803,aa:Asn,seq:gtt)
     gene            8157..14107
                     /gene="yeeJ"
                     /locus_tag="KLK57_002803"
                     /pseudo
     CDS             8157..14107
                     /gene="yeeJ"
                     /locus_tag="KLK57_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
BASE COUNT         3991 a         3353 c         3170 g         3593 t
ORIGIN      
        1 tgaagcccta tgttaatttc tttgcggtaa cataatccgg aacacattat gcttattgag
       61 atagtgatac gtagcacttc ccccatgtaa ttcggcaatc gctttgacta aagaaagacc
      121 aagtccctga cctacggaat gacgcgaatt atctccccgc caaaatctac ggaagagttt
      181 ttcaggctca tgaattttcg ttccaggact ggcgacatca atattaagat agccgttggt
      241 atcaagaaaa ctggttatat gaatacgcga tttttctggt gaatatctaa tggcattaac
      301 aataagattc gataacattc gttgcagtaa aattttatcc gcaaagattt gctgattgca
      361 ctcgacctta aagcaaatct ctttctcgtc tgaaaggtat tcaagataat ccaacaaatt
      421 ttcgacttcc ttattgagag aaagcgagtc cagtttcacc aaaacattgt ttttatctgc
      481 ccgggcaaga aacagtatgt tctctgttaa ccgcgaaata ttttccagct cttcaatgtt
      541 accggcaatt gttttttgat attcagcgat acttctggtt tgactgagcg taacctgatt
      601 ctgacccagt aatgcattaa tgggcgttct aagttcatga gcgagatcgt cagcaaattg
      661 acttagacgc tcaaaatctt tgactaaagc atgatgcatt ttattcaacg cctgcccaag
      721 aggttttagt tctctcggta atgcgctaac ctcaacaggc tcccggctat cgttataatt
      781 cagcgcttcc gttacaccac tcaacttttt gatctctcgt aatcccgttc tgattaacag
      841 cggacttaat actgagcaaa gtacaatggc gacaatgcaa attataatgc tattaatttt
      901 atactgttca agcatgttat gtctggctga agccaattta gccacagtaa ccgttaatga
      961 tggcgaaact tcatcaatat taattcgtaa agcatcaatc tcagtatcat taataatgct
     1021 tctgtaaatg ccagctgcgc tgattgtctc actagcaggt atgttattta acatgtcatc
     1081 actgacattt gtccggttaa caattttatt gatgccatca ccatgaatga tcaagatatc
     1141 ctgactaaca tccatcatcc ggttaaagta tacaggtaac gtatctggat ttaccccatc
     1201 aattaacaac tgcttgatct gtgctgtccg gttaatgagt gttgtatcat cgcgccattt
     1261 caactcactt gccaggccat tatagagagt ccagacaatt ccagcgccag caacagacag
     1321 cagcaatata aaaagcaagg ttaaacggac ggttatagat aatcttttca ttattttttt
     1381 accgctacga atgaatagcc catcccccgg attgtggcga ttagcttttc aggaaaagga
     1441 tcatcaactt ttgcgcggag cctgcgaatg gcgacatcca ccgtattggt atcactatca
     1501 aagttgattc cccaaatttc actcgcaata accgttctgg gtataatttc gccagctctg
     1561 gaggccagta gccaaagtaa ctgaaactcc ttgcgcgtca gtgtaatact gatattgttc
     1621 ctgctcacac tttgacttac agagtccatt cttaagccac tgatttctaa tgttgaattc
     1681 aaagcgtgat gttgccttaa ttgtgcccga acccttgcca gcaactcaga aaatgaaaaa
     1741 ggttttacca gataatcatt tgctccactg tccagccctc tgactctgtc atcgacagaa
     1801 tcccttgcag taaggcaaat aacaggggtt tgctttgctg ttcttaacgt ttgtaagatc
     1861 tgccagccat ccatacccgg aagcataata tccagaatga tcaatgcata atcatccttc
     1921 agcgcaagat aaagcccatc tctgccatca gaaacggcat caatgacata acccgcttcg
     1981 gaaagcccct gcgttaccca ctcctgggtc ctttgattat cttcaataag taaaatcttc
     2041 atgaaaataa tatgccataa atcatatgtt atagaaacag cctagttcat tacaaaattg
     2101 taatgctgct gtaaggttac cctggccgct ttttcgctat cctcaaaact cattcacatg
     2161 acaaggatat aaacatgtta aagcgttatt tagtactctc cgtagtaacg gcagcatttt
     2221 cattaccttc tttggtttat gccgcacaac aaaacattct tagcgtgcac attttgaacc
     2281 agcaaaccgg aaaacccgct gccgacgtga cagtcactct tgaaaagaaa gcggacaacg
     2341 gctggttaca acttaatacc gccaaaacag ataaggatgg acgaattaag gcactgtggc
     2401 ccgagcaaac tgcaactacg ggcgattacc gtgtcgtatt taaaaccggg gactatttca
     2461 agaaacaaaa tcttgaaagt ttcttccctg agatccccgt tgaatttcat attaataaag
     2521 tgaacgagca ttatcatgtg cctttattac ttagccaata tgggtattca acctatcgtg
     2581 gcagttaatg cataaatatg taaaagcaga tctctgctac tgttagggag gacactccct
     2641 gacagattaa cagtaaacgg ctcttgctgg ctaacgacaa aaaagtgtga tggcttatga
     2701 aaaagaatca atttttaaaa gaatcagatg ttacggccga gtcggtattc tttatgaagc
     2761 gtcgacaggt gttaaaagca ctgggcatca gcgcagctgc actttctttg cctcacgctg
     2821 cgcatgccga tctgcttagc tggtttaaag ggaacgatcg cccgcccgcc cccgccggaa
     2881 aaccgctgga gttcagcaag cctaccgcct ggcaaaataa tctgccactg acgccagtag
     2941 ataaagtctc cggttataac aacttctatg aattcgggct ggataaagcc gatcccgccg
     3001 ctaatgctgg tagcctgaaa accgatccat ggacactgaa aatcagcggc gaagtggcaa
     3061 aaccattgac cctcgatcac gatgatttaa cccgtcgctt cccgctggaa gagcgtattt
     3121 atcgtatgcg ctgcgtggag gcatggtcga tggtggtgcc gtggattggt tttccgctgc
     3181 acaaattgct ggcgcttgcc gaacccacca gcaatgcgaa gtatgtcgct ttcgaaacaa
     3241 tttatgcacc ggaacaaatg ccaggccagc aggaccgctt tatcggcggc gggctgaaat
     3301 atccttatgt cgaaggattg cgtctcgacg aagcaatgca tccgctcaca ctgatgaccg
     3361 tgggtgttta tggcaaggcg ttaccgccac aaaatggcgc gccggtacga ctgattgtgc
     3421 cgtggaaata tggctttaaa gggattaaat cgatagtcag tattaagctg acccgcgagc
     3481 gtccgccaac cacctggaat ctggcagcgc ctgacgaata cggtttttac gccaacgtta
     3541 atccgcatgt tgatcacccg cgctggtcac aggctaccga acgatttatt ggttcaggcg
     3601 gcatcctcga tgttcagcgc cagccaacgc tactgtttaa tggttacgcc gaccaggtgg
     3661 catcgctgta tcgtggcctg gatttgcggg agaatttctg aatgcgtctg acagcaaaac
     3721 aggtgacatg gctgaaagtt agcctgcatc ttgccggatt gttgccgttt ctctggctgg
     3781 tctgggcgat caatcacggt ggactgggtg ccgatccggt gaaagatatt cagcatttta
     3841 ccggtcgcac tgcactgaaa tttttgctgg cgaccttgtt aatcacacct ctggcacgct
     3901 acgcaaaaca gccgttattg atacgcactc gccgcctgtt aggcttatgg tgctttgcct
     3961 gggcgacact gcatttaacc agttacgcat tgctggagct gggcgtgaat aatctggcgt
     4021 tattaggcaa ggagttaatt acccggcctt atttaacgtt aggaattatc agctgggtta
     4081 ttttgcttgc tttagcgttt acttcaaccc aggcgatgca gcgaaaactc ggcaagcact
     4141 ggcaacagtt gcataatttc gtctatcttg tcgcgatcct ggccccaata cattatctgt
     4201 ggtcagtgaa gattatttca ccgcagccgc tcatctacgc tgggctggct gtactgcttt
     4261 tagccttacg gtataagaag ttgcgttccc tttttaagcg gttacgcaag caggttcaca
     4321 acaaattatc tgtataactt catattgccg acaaagtacg cgttatccct gccggatgtg
     4381 gcgcgagcgc cctgtccggc ctgcgcagat cacatcaggt tatgtttcgt ttgtcatcaa
     4441 cctcaaatta tcaacataat tcattttatt tcaataagat agataagtac acctgagaaa
     4501 actatgctct catctcctaa gagttgttgc attttgccat atgttacaat ataacattac
     4561 acatcatata caatgactct ggaggaaact gttttggcaa ttcgtcttca caaactggct
     4621 gttgctttag gtgtctttat tgttagcgct cctgcctttt cgcatggtca tcactcacac
     4681 ggcaaaccct taacagaggt cgaacaaaaa gctgctaatg gtgtttttga tgatgccaat
     4741 gtacaaaacc gaacgctcag tgactgggat ggagtctggc aatccgttta tcctttactg
     4801 caaagtggca aacttgaccc cgtctttcag aagaaagcgg atgcagataa aattaaaaca
     4861 tttgctgaaa ttaaagatta ttatcacaaa ggttatgcaa cagatatcga gatgattggc
     4921 attgaggacg gcattgttga attccataga aataatgaaa caacatcctg taaatatgat
     4981 tacgatggat acaaaatact cacctataaa tcaggcaaga aaggcgttcg ttacttattc
     5041 gaatgtaaag atcctgaaag caaagcccct aaatatatac aatttagcga tcatataatt
     5101 gcaccacgaa aatcatctca ttttcacatt tttatgggta atgattcaca gcaattttta
     5161 ttaaatgaaa tggaaaactg gccaacgtat tatccatatc agttgagtag cgaagaagtg
     5221 gtcgaggaaa tgatgtctca ttgagtattc tcatgataac gcctcgatgc cgctttagta
     5281 agtcatcata actgccactg gtccatccac aaacgccact gaacgcaagc tagctacaga
     5341 cacgctcatc actatgacgt gtctgtatat taataagcta acccgcattg agttaaccaa
     5401 taacggattc catacacaat acggccaaca gagaaagata cctgtgctca cgccattgct
     5461 tatattggct ggttacaatg tgcactatca attttttaaa taacaaattc ttaaaaccac
     5521 atctgtataa ggaattttta aggttcgtgg gatagcttga ctgtaaaaat tacaggagct
     5581 acaaaaatga accgattctc aaaaactcaa atttatttac attggataac gctgcttttc
     5641 gttgcaataa cctatgccgc gatggaactc cgtggctggt ttcctaaagg tagtagtact
     5701 tatctgctga tgcgagaaac acattacaat gcgggtatat tcgtttgggt gttaatgttt
     5761 tcacgcctga ttataaaaca ccgttatagt gatccttcta ttgtaccacc gccacctgcc
     5821 tggcaaatga aagcggcttc gctaatgcac atcatgcttt atataacctt ccttgcactt
     5881 cctctgctgg ggattgcttt gatggcttac agtggaaaat cgtggagttt ccttggtttc
     5941 aatgtgtctc cctttgttac cccaaacagc gaaattaaag cactgataaa aaatattcac
     6001 gaaacctagg caaatatagg ttacttttta atcgcagctc acgctggcgc agcactcttt
     6061 catcactaca ttcagaaaga taatactctg ttacgaatga tgcatcgccg caaataagtc
     6121 gatcccctga caaggaaatg tgaaccatgt acatcaacgc gtcataaatg tttacgcaag
     6181 tgcacaatgc atcctttcta ctatcaacaa ggaggagcag aaactgtgta aatcgtacaa
     6241 tggtaagact tatcaaaaat acaggttaat tttggtattt aaaccttttt tacttgttag
     6301 tgtgatacaa aggctttcaa aaaagctggg aaattcaaca actcttggaa attatggact
     6361 ttgtcccgcg gctttaatct gacggccgcg ttcctttttt gactcaatta ttgacccctt
     6421 ctctacgcaa cttcagttcc caccaccaac tttgcggcag tttcatagga tgaatgtcta
     6481 aaagaataat ggtgagcgat aagaaaacga ctgaataact gcacattttc gctcgaaagc
     6541 ttcctgtcag atccatagcg aatcaagtgc tgaatgtcac agtatcgaac agaaaacagt
     6601 gacgatctaa cccttcaaga atattctaca attgttctgt ttaggaaaag caaggcggga
     6661 agtcaggaga taagtcattg ataaagtggc ggagagaggg ggatttgaac ccccggtaga
     6721 gttgccccta ctccggtttt cgagaccggt ccgttcagcc gctccggcat ctctccgttc
     6781 agatggttgc catgatgcca ggaaatttgg cattttaaca gtccctgttc gtgcaatttt
     6841 gttcaagtga cgagtttgcg agcaaaacga tgattaagtg gccctggaaa gtacaagaat
     6901 cagcacatca aactgccctt ccctggcagg aagcactatc gatccccctt ttaacgggtc
     6961 tgacggaaca ggaacaaagc aaattagtca ctcttgccga acgtttttta cagcaaaagc
     7021 ggcttgttcc tttacagggc tttgaactgg attcattaag aagctgccgg atagcacttc
     7081 tattttgcct acccgttctg gagttaggac tggaatggct ggatggtttt catgaagtct
     7141 taatttatcc tgcgccattt gtggtcgatg atgaatggga agacgatatc ggtctggtgc
     7201 ataaccaacg tattgttcag tcaggtcaga gctggcagca agggcctatc gttttgaact
     7261 ggttggatat acaagattct tttgatgcat ctggttttaa cctgattatt catgaagtcg
     7321 ctcataagct ggacacccgt aacggcgatc gcgccagcgg agttcccttt attccgttgc
     7381 gtgaggttgc tggctgggaa cacgatcttc atgctgcaat gaacaacatt caggaagaaa
     7441 tcgaattggt tggcgagaat gcggcgagca ttgatgctta tgcagccagc gatcctgctg
     7501 aatgttttgc cgtactttct gaatatttct ttagcgcccc agaacttttt gctcctcgtt
     7561 tcccttcatt gtggcaacgt ttctgccaat tttatcaaca agatcctttg cagagactgc
     7621 atcacgctaa tgatacagac tcgttttcgg cgacgaatgt tcattaatta acaactttgc
     7681 agattaatta accaattgaa atgacttatg aaatttagtg ttgacagaca aggtaccgct
     7741 aagtaatatg tgccccgttc acacgattcc tctgtagttc agtcggtaga acggcggact
     7801 gttaatccgt atgtcactgg ttcgagtcca gtcagaggag ccaaattcta aaaattcgct
     7861 tttttagcgc aatgtcactg accttagttg aacattgttt tttaacggat agcgggtttt
     7921 taacacctta agcgccctcg acctttatgg ttgagggcgt tttgctatga acgccatcac
     7981 cattttcccc tcgattataa aacttgagtt attcagtagt ctcccctctt gcaactcaca
     8041 cccaaaactg cctaacgaaa agttattaat tttcaatcgt attgctatca gtatttacat
     8101 tttttcgctg tgctagaaag ggcgcattta tgttagctcg ttcagggaag gtaagcatgg
     8161 ctacgaagaa gagaagtgga gaagaaataa atgaccgaca aatattatgc gggatgggaa
     8221 ttaaactacg ccgcttaact gcgggtatct gtctgataac tcaacttgcg ttccctatgg
     8281 ctgcggcagc acaaggtgtg gtaaacgccg caacccaaca accagttcct gcacaaattg
     8341 ccattgcaaa tgccaatacg gtgccctaca cccttggagc gctggaatcg gcccaaagcg
     8401 ttgccgaacg tttcggtatt tcggtggctg agttacgcaa actcaaccag tttcgtacgt
     8461 ttgctcgagg ttttgataat gtccgccagg gtgatgaact ggatgtcccg gcacaagtta
     8521 gtgaaaaaaa attaaccccg ccgccgggta ataacagtga caacctcgag caacagatag
     8581 ccagtacttc acagcaaatc gggtctctgc tcgccgaaga tatgaacagc gagcaagcgg
     8641 caaatatggc gcgtggatgg gcctcttctc aggcttcagg cgcaatgaca gactggttaa
     8701 gccgcttcgg taccgcaaga atcacgctgg gcgtggatga agattttagc ctgaagaact
     8761 cccagttcga ttttctccat ccgtggtatg aaacgcctga taatctcttt ttcagtcagc
     8821 atactctcca tcgtactgac gagcgtacgc agattaacaa cggcttaggt tggcgtcatt
     8881 tcactcccac atggatgtcg ggcatcaact tctttttcga ccacgatctt agccgttacc
     8941 actcccgcgt cggcattggc gcggagtact ggcgcgacta tctaaaatta agcagtaacg
     9001 gctatttgcg actgaccaac tggcgcagcg cacctgaact ggacaacgat tataaagcac
     9061 gcccggccaa tggctgggat gtacgcgcag aaagctggct acccgcctgg ccgcaccttg
     9121 gcggtaaact ggtctatgaa cagtattatg gcgattaagt ggccctgttc gataaagacg
     9181 atcggcaaag taatcctcat gccataaccg ctggacttaa ctataccccc ttcccgctga
     9241 tgaccttcag cgcggagcaa cgccagggta aacagggcga aaatgacacc cgttttgccg
     9301 tcgattttac ctggcaacct ggcagcgcaa tgcagaaaca gcttgacccg aatgaagtcg
     9361 ctgcacggcg tagccttgca ggcagccgtt atgatctggt ggatcgcaac aacaatatcg
     9421 ttctggaata tcgcaaaaaa gaactggttc gcctaaccct gacagacccc gtgacaggga
     9481 agtcaggaga agtgaaatca ctggtttcgt cgctacaaac caaatatgcc ctgaaaggct
     9541 ataacgtcga agccaccgca ctggaagctg ccggtggcaa agtggtcaca acgggtaaag
     9601 atattctagt taccctgccg gcttaccggt tcaccagtac gccagaaacc gataacacct
     9661 ggccgattga agtcaccgcc gaagatgtca aaggcaattt gtcgaatcgt gaacagagca
     9721 tggtggtcgt tcaggcacct acgctaagcc agaaagattc ctcggtatcg ttaagtaccc
     9781 aaacattgaa cgcggattcc cattcaaccg ccacactgac ttttattgcg catgatgcag
     9841 caggtaatcc tgttgtcggg ctggtgctct cgacgcgtca cgaaggtgtt caggacatca
     9901 ccctttctga ctggaaagat aatggtgacg gaagctatac ccagatcctg accacaggag
     9961 cgatgtctgg cacgctgacg ctgatgccac agctgaatgg tgtggatgcg gctaaagccc
    10021 ccgccgtggt gaatatcatt tctgtttcgt catcccgaac tcactcgtca attaagattg
    10081 ataaggaccg ttatctctcc ggcaatccta tcgaggtgac ggtagaactg agagatgaaa
    10141 atgacaaacc tgttaaggaa caaaaacagc aactgaataa cgcagtcagc atcgacaacg
    10201 tgaaaccagg agtcactaca gactggaaag aaaccgcaga tggcgtctat aaggcgacct
    10261 ataccgccta taccaaaggc agtggactta ctgcgaagct attggaatga agatttgcat
    10321 accgctggat ttatcatcga cgccaacccg cagtcagcga aaattgcgac attatctgcc
    10381 agcaataatg gtgtactcgc caatgagaat gcagcaaaca ccgtctcggt caatgtcgct
    10441 gatgaaggaa gcaacccaat caatgatcat accgtcacgt ttgcggtatt aagcggatcg
    10501 gcaacttcct tcaacaatca aaacaccgca aaaacggatg ttaatggtct ggcgactttt
    10561 gatctgaaaa gtagtaagca ggaagacaac acggttgaag tcacccttga aaatggcgtg
    10621 aaacaaacgt taatcgtcag ttttgtcggc gactcgagta ccgcgcaggt tgatctgcag
    10681 aagtcgaaaa atgaagtggt tgctgacggc aatgacagcg ccacaatgac cgcgaccgtc
    10741 cgggatgcaa aaggcaacct gctcaatgac gtcatggtca ctttcaatgt taattcagca
    10801 gaggcgaaac tgagccaaac cgaagtgaat agccacgacg ggatcgccac agctacgctg
    10861 accagtttga aaaatggtga ttatagggtt acggcctctg tgagctctgg ttcccaggct
    10921 aatcaacagg tgaattttat cggtgatcaa agtactgctg ccctgaccct cagtgtgcct
    10981 tcaggtgata tcaccgtcac caacacagct ccgcaacata tgactgcaac cttgcaggat
    11041 aaaaatggca acccactaaa agataaagaa atcaccttct ctgtgccaaa cgacgtcgca
    11101 agtaagttct cgattagcaa cggaggaaaa ggcatgacgg atagtaacgg ggttgcaatc
    11161 gcctccctga ccggcacgtt agcgggcacg catatgatca tggctcgtct ggctaacagc
    11221 aatgtcagcg atgcacagcc aatgacgttt gtggcggata aagacagagc ggttgtcgtt
    11281 ttgcaaacat cgaaagcgga aatcattggg aatggcgtgg atgagacaac tctgacagca
    11341 acagtgaaag atccgtcgaa tcatccggtg gcggggataa cggtaaactt caccatgcca
    11401 caggacgttg cggcaaactt tacccttgaa aataacggta ttgccatcac tcaggccaat
    11461 ggggaagcgc atgtcacgct gaaaggtaaa aaagcgggca cgcatacggt taccgcaacg
    11521 ctgggtaata acaataccag tgattcgcag ccggtaacat ttgtggcgga caaagcctcg
    11581 gctcaggttg tcctgcagat atcaaaagat gagatcacag gtaatggcgt cgatagcgca
    11641 acgctaactg caacggttaa agatcagttc gacaatgagg tgaataatct tccggtaaca
    11701 ttcagctcag cctcttcagg actcaccctg accccgggag taagtaatac caatgagtct
    11761 ggcatcgcgc aggccactct cgcaggcgtt gcctttggtg agcagacggt cactgcatca
    11821 ctggctaata atggtgccag cgacaacaaa actgtgcatt ttattggcga cacagcggcg
    11881 gcaaaaatta tcgagttgac gcctgtccca gacagcataa tcgccggtac cccgcagaac
    11941 agctccggca gcgccatcac cgccacagtc gttgataata atggctttcc ggtgaaaggt
    12001 gtgactgtga acttcaccag cagaacaaac tctgccgaaa tgacgaatgg cggccaagcc
    12061 gtaacgaacg aacagggtaa ggctaccgtc acttatacca atacccgctc ctcgatagaa
    12121 tcaggagcga gaccggatac cgttgaggcc agtctggaaa atggtagctc cacgcttagc
    12181 acatcaatta atgtcaacgc tgatgcgtct acggcacatc tcacctggct acaggcactt
    12241 tttgatacag tctccgcagg cgagacaacc agtctgtata ttgaggtgaa ggataattac
    12301 ggcaacggtg tcccccagca tcaggtgacc ctcagcgttt caccaagtga aggcgtgacc
    12361 cccagtaata acgctatata tactaccaac cacgacggca atttttacgc aagctttacc
    12421 gctacaaaag ccggggttta tcaagtgacg gcaaccctcg aaaatggcga ttcgatgcaa
    12481 caaacagtga cctatgtgcc gaacgtcacg aatgccgaaa tcacgctggc aacctcgaag
    12541 gatccggtga ttgccgacaa taacgatctc acgacactaa cagcaacagt cgctgataca
    12601 gagggcaatg cgatagccaa cactgaagta acatttactc tgccggaaga tgttaaggcg
    12661 aacttcacgc tgagcgatgg cggtaaagcg attactgatg ctgaaggcaa agcgaaagtc
    12721 acgctgaaag gtacaaaagc aggcgctcat actgttacag catcgataac tggcggtaag
    12781 agtgagcagt tggtggtgaa ctttactgcg gatacgctca ctgcgcaggt taatcttaac
    12841 gttaccgagg acaattttat cgctaataac gtcgggatga ccaggctgca ggcaacagtg
    12901 actgatggaa acggcaaccc gttagccaat gaggcggtga cattcacgct accggcagat
    12961 gtgagcgcaa gctttactct cggacaaggc ggttccgcca ttactgacat caacggcaag
    13021 gctgaagtta cactgagcgg tacaaaatcc ggcacctacc ccgtgacagt tagcgtgaac
    13081 aattatggtg tcagtgatac gaaacaggtg actttgattg ccgatgctgg taccgcaaca
    13141 ctagcctcct taacctctgt atactcattc gtcgtcagca cgaccgaagg cgcgaccatg
    13201 actgcaagcg tcactgacgc taacggcaac ccggtagaag gcataaaagt taatttccgc
    13261 ggaacctccg tcacgctaag cagcaccagc gttgaaacgg atgatcgggg tttcactgaa
    13321 attcttgtga caagcaccga agtcggactg aaaacagttt cagcctctct ggcagataaa
    13381 cctactgaag tcatatcgcg attactgaat gcaaaagcag atattaattc tgcaacgatt
    13441 accagtctgg agatacctga aggtcaggtc atggtcgcac aagacgtagc agttaaagct
    13501 cacgtcaacg accagtttgg caacccgatt cttaatgaat ctgtaacatt cagtgcagag
    13561 ccaccagagc acatgaccat cagccaaaat attgtctcta ctgatacgca tggtatagcc
    13621 gaggtctcca tgacgcccga aagaaacggt tcgtatatgg tgaaagcatc cctggcgaat
    13681 ggagcctcac ttgagaaaca actggaggct attgatgaaa aactgacact ctcggcgtcc
    13741 agcccgctta tcggtgtcaa ttccccaaca ggtgcaactc tgacggcaac gctaacttct
    13801 gcaaatggca ctccagtgga gggtcaggtc atcaacttta gcgtaacgcc agaaggtgcg
    13861 acgttaagtg gcggaaaagt gagaactaac tcttctggtc aggctccagt cgttctgacc
    13921 agcaataaag tcggtacata tacggtgact gcatcgttcc ataacggcgt aacaatacag
    13981 acacagacaa ccgtgaaagt cactggcaac tcaagcaccg cccatgttac tagctttatc
    14041 gctgatccat cgactatcgc cgccaccaac agtgatttaa gtaccttaaa ggcaacggtt
    14101 gaggatg
//
LOCUS       DAEEKQ010000101        14001 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.101, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000101 DAEEKQ010000000
VERSION     DAEEKQ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.101"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            75..2216
                     /gene="ldcC"
                     /locus_tag="KLK57_002804"
     CDS             75..2216
                     /gene="ldcC"
                     /locus_tag="KLK57_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBE0893122.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVEMAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            2273..2662
                     /locus_tag="KLK57_002805"
     CDS             2273..2662
                     /locus_tag="KLK57_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE0893123.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            2727..4025
                     /gene="tilS"
                     /locus_tag="KLK57_002806"
     CDS             2727..4025
                     /gene="tilS"
                     /locus_tag="KLK57_002806"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HBE0893124.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVRHCENICQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWIKSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(4074..4334)
                     /gene="rof"
                     /locus_tag="KLK57_002807"
     CDS             complement(4074..4334)
                     /gene="rof"
                     /locus_tag="KLK57_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HBE0893125.1"
                     /translation="MSMNDAYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(4321..4521)
                     /locus_tag="KLK57_002808"
     CDS             complement(4321..4521)
                     /locus_tag="KLK57_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HBE0893126.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            4687..5232
                     /locus_tag="KLK57_002809"
     CDS             4687..5232
                     /locus_tag="KLK57_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HBE0893127.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADYEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAKV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            5229..5590
                     /gene="arfB"
                     /locus_tag="KLK57_002810"
                     /pseudo
     CDS             5229..5590
                     /gene="arfB"
                     /locus_tag="KLK57_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
     gene            5664..6374
                     /gene="nlpE"
                     /locus_tag="KLK57_002811"
                     /gene_synonym="cutF"
     CDS             5664..6374
                     /gene="nlpE"
                     /locus_tag="KLK57_002811"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HBE0893128.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(6529..7353)
                     /locus_tag="KLK57_002812"
     CDS             complement(6529..7353)
                     /locus_tag="KLK57_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HBE0893129.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITVPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIYLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSSYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSGLAKAQLSSVGEGDKKSWFCSEFVTDAFAKA
                     GHPLTLAQSGWISPADLMHMRTGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(7406..9124)
                     /gene="proS"
                     /locus_tag="KLK57_002813"
     CDS             complement(7406..9124)
                     /gene="proS"
                     /locus_tag="KLK57_002813"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HBE0893130.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="KLK57_002814"
     CDS             complement(9235..9942)
                     /gene="tsaA"
                     /locus_tag="KLK57_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HBE0893131.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="KLK57_002815"
     CDS             complement(9939..10343)
                     /gene="rcsF"
                     /locus_tag="KLK57_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HBE0893132.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTLGCYRQAVCIGSALNITAK"
     gene            complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="KLK57_002816"
     CDS             complement(10461..11276)
                     /gene="metQ"
                     /locus_tag="KLK57_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HBE0893133.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(11316..11969)
                     /gene="metI"
                     /locus_tag="KLK57_002817"
     CDS             complement(11316..11969)
                     /gene="metI"
                     /locus_tag="KLK57_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HBE0893134.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(11962..12993)
                     /gene="metN"
                     /locus_tag="KLK57_002818"
     CDS             complement(11962..12993)
                     /gene="metN"
                     /locus_tag="KLK57_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HBE0893135.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            13181..13756
                     /gene="gmhB"
                     /locus_tag="KLK57_002819"
     CDS             13181..13756
                     /gene="gmhB"
                     /locus_tag="KLK57_002819"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HBE0893136.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         3401 a         3544 c         3717 g         3339 t
ORIGIN      
        1 cacttcggtg gccctttttt atcgccacgg tttgagcagg ctatgattaa ggaaggattt
       61 tccaggagga acacatgaac atcattgcca ttatgggacc gcatggcgtc ttttataaag
      121 atgagcccat caaagaactg gagtcggcgc tggtggcgca aggctttcag attatctggc
      181 cacaaaacag cgttgatttg ctgaaattta tcgaacataa ccctcgaatt tgtggcgtga
      241 tttttgactg ggatgagtac agtctcgatt tatgcagcga tatcaatcaa cttaatgaat
      301 atctcccgct ttatgccttc atcaacaccc actcgacgat ggatgtcagc gtgcaggata
      361 tgcggatggc gctctggttt tttgaatatg cgctggggca ggcggaagat atcgccattc
      421 gtatgcgtca gtacaccaac gaatatcttg ataacattac gccgccgttc acgaaagcct
      481 tgtttaccta cgtcaaagag cggaagtaca ccttttgtac gccggggcat atgggcggca
      541 ccgcatatca aaaaagcccg gttggctgcc tgttttatga ttttttcggc gggaataccc
      601 tcaaggctga tgtctctatt tcggtcaccg agcttggttc gttgctcgac cacaccgggc
      661 cacacctgga agcggaagag tacatcgcgc ggacttttgg cgcggaacag agttatatcg
      721 ttaccaacgg aacatcgacg tcgaacaaaa ttgtgggtat gtacgccgcg ccatccggca
      781 gtacgctgtt gatcgaccgc aattgtcata aatcgctggc gcatctgttg atgatgaacg
      841 atgtagtgcc agtctggctg aaaccgacgc gtaatgcgtt ggggattctt ggtgggatcc
      901 cgcgccgtga atttactcgc gacagcatcg aagagaaagt cgctgccacc acgcaggcac
      961 aatggccggt tcatgcggtg attaccaact ccacctatga tggcttgctc tacaacaccg
     1021 actggatcaa acagacgctg gatgtcccgt cgatccactt cgattccgcc tgggtgccgt
     1081 acacccattt tcatccgatc taccagggga aaagtggtat gagcggcgag cgtgttgcgg
     1141 gaaaagtgat cttcgaaacg caatcgaccc acaaaatgct ggcggcgtta tcgcaggctt
     1201 cgctgatcca cattaaaggc gagtatgacg aagaggcgtt taacgaagcc tttatgatgc
     1261 ataccaccac ctcgcccagt tatcccattg ttgcttcggt tgagatggcg gcggcgatgc
     1321 tgcgtggtaa tccgggcaaa cggctgatta accgttcggt ggagcgggcg ctgcattttc
     1381 gcaaagaggt ccagcggttg cgggaagagt ctgacggttg gtttttcgat atctggcaac
     1441 cgccgcaggt ggatgaagcc gaatgctggc ccgttgcgcc aggcgaacag tggcacggct
     1501 ttaacgatgc ggatgccgat catatgtttc tcgatccggt taaagtcact attttgacac
     1561 cggggatgga cgaacagggc aatatgagcg aggaggggat cccggcggcg ctggtggcaa
     1621 aattcctcga cgaacgtggg atcgtagtag agaaaaccgg cccttataac ctgctgtttc
     1681 tctttagtat tggtatcgat aaaaccaaag caatgggatt attgcgtggg ttgacggaat
     1741 ttaaacgctc ttacgatctc aacctgcgga tcaaaaatat gctacccgat ctctatgctg
     1801 aagatcccga tttctaccgc aatatgcgta ttcaggatct ggcgcaaggg atccataagc
     1861 tgattcgtaa acacgatctt cccggtttga tgttgcgggc attcgatact ttgccggaaa
     1921 tgatcatgac gccacatcag gcatggcaga gacagattaa aggtgaagta gaaaccattg
     1981 cgctggaaca actggtcggt agagtatcgg caaatatgat cctgccttat ccaccgggcg
     2041 taccgctgtt gatgccagga gaaatgctga ccaaagagag ccgcacggta ctcgattttc
     2101 tactgatgct ttgttccgta gggcaacatt accccggttt tgaaacggat attcacggcg
     2161 cgaaacagga cgaagacggc gtttaccgcg tacgagtcct aaaaatggcg ggataacttg
     2221 ccagagcggc ttccgggcga gtaacgtgct gttaacaaat aaaggagacg ttatgctggg
     2281 tttaaaacag gttcaccata ttgcgattat tgcgacggat tatgcggtga gcaaagcttt
     2341 ctactgcgat attcttggtt tcacgctgca aagcgaagtc tatcgcgaag cgcgcgactc
     2401 gtggaaaggg gatttggcgc ttaatgggca atatgtgatt gagcttttct cttttccgtt
     2461 cccgccggag cgccccagcc gaccggaagc ttgcggcctg cgtcatctgg cttttagcgt
     2521 tgatgatatc gatgcggcag tggcgcacct tgaaagccat aacgtgaagt gtgaagccat
     2581 ccgtgtcgat ccatacacgc aaaaacgctt cacgttcttt aacgatccgg acgggctgcc
     2641 gttggaactg tatgagcagt aaggcttgtc atcgccgcat ttgcccggta acgtgccggg
     2701 cattgctact gtaaaatcgc accatcatga cactcacgct caatagacaa cttctcacct
     2761 cacgccagat tctggtggcc tttagcggcg ggcttgactc caccgttctg ctgcatcagt
     2821 tggtgcagtg gcggacggaa aatccgggcg tcactctacg cgctatccat gtgcatcatg
     2881 gtttaagtgc taatgccgat gcctgggtta ggcattgcga gaacatctgc caacagtggc
     2941 aggtgccgct ggtggtcgag cgcgtgcaac ttgcgcaaga aggactgggc attgaggccc
     3001 aggcgcggca ggcgcgttat caggcatttg ctcgtaccct gttacccggt gaagtgctgg
     3061 tcaccgcgca acatctcgac gatcaatgtg aaacctttct gctggcgcta aaacgcggta
     3121 gcggccctgc cgggctttcg gctatggggg aagtttcgga atttgccgga acacggctta
     3181 tccgcccgtt gcttgcccgc acgcgggggg aactggagca gtgggcattg gcgcatggtt
     3241 tacgctggat tgaagacgaa agtaatcagg acgactcata cgatcgtaac tttctgcgcc
     3301 tgcgtgtagt gccgttattg cagcagcgtt ggccgcattt tgccgaagca acggcccgca
     3361 gcgccgcact ttgtgctgaa caagagagtc tgttggatga actgctggcg gatgatttag
     3421 cacactgcca aacgtcgcag gggacgctgc agattgcgcc aatgctggcg atgagtgatg
     3481 cccgtcgcgc ggcgattatc cgccgctggc tggcagggca gaatgcaccg atgccttccc
     3541 gcgacgcgct ggtgcggatc tggcaggaag tggcgctggc gcgggaagat gcctccccct
     3601 gtttacgttt tggcgcgttt gaaatacggc gctatcagtc gcaactgtgg tggattaaat
     3661 ccgtcactgg gcaaagcgaa accattgtgc cgtggcagac gtggctacaa ccgctggaat
     3721 taccggcggg gctgggaagt gtacagctta ctgcgggagg cgatattcgc cctccgcgtg
     3781 cagacgaagc ggtcagcgtg cgtttcaaag cgccaggatt gctgcatatt gtcgggcgta
     3841 acggcggacg taagctaaag aaaatctggc aagagctggg cgtgccgccg tggctacgtg
     3901 acaccacgcc actgctgttt tatggcgaaa cgctgattgc ggcggcaggg gtatttgtga
     3961 cgcaagaagg tgtggctgaa ggtgagaatg gcgtaagttt tgtctggcag aaaacgctta
     4021 gttaagtgaa agccggataa gacgcatcaa acgtcgcatc cggcgaaagt caatcaggat
     4081 tcgcttacca ccaccgtacc gatttccggg tggctaaagc tggtaatttt atccagacgc
     4141 agctcgcggg tttcgccagc ggcctcgacg accagatact ccacattttt gcgggagact
     4201 aaatcactgg cttttgcctg caatttttcg ccatctttca gctcaagtgt cagcattaaa
     4261 tgatgctggc aggcgagctc aagattatcg taatcatcac aattgattgg ttgatacgca
     4321 tcattcattg acataatcgc tcaccagtaa gtttgccgca gcgtatgctg ctttttccct
     4381 gacagcctca gaaagggcgt cgtcggcagc catttcattc agcactttca aaacgcagcc
     4441 cagcgcgtcc ggaacgtatc ctaagtctcc gctggcgatt tccgcgtacc gcttgcgtat
     4501 taactcacaa tatttttcca catgccctcc tgtcagcact ctgacttaac cgtggatgca
     4561 agtctaagcc tacgaagata aactctgttt cgcaaggtga ctataccaca ctcatttctg
     4621 caatatcagc gccgcaactg cacgtattcc gttacaatgg cctcctgatt cgaaaggagt
     4681 tttcttatgg cgcttaaagc gacaatttat aaagcgacgg ttaatgtggc cgatctcgac
     4741 cgcaaccagt ttctcgatgc ctctctgacg ctggcgcgcc acccttcaga aacccaggag
     4801 cgtatgatgc tgcgcctgct ggcgtggctg aaatatgctg atgaacgtct gcaatttacc
     4861 cgtggtttgt gtgccgatta tgagccggaa gcgtggctgc gtaacgatca cctgggcatt
     4921 gatttgtgga ttgagctggg gctgccggat gagcggcgga ttaagaaagc ctgcacccag
     4981 gccgcaaaag tggcgctgtt tacctataat agccgggcgg cgcaaatctg gtggcagcaa
     5041 aatcagagca aatgtgtgca gtttgccaat ctttccgtct ggtatctgga cgatgaacaa
     5101 ctggcgaaag taagcgcctt tgccgatcgt accatgacgc tgcaggcaac gattcaggat
     5161 ggcgtgatct ggttatcgga tgataagaat aatctggaag tgaacttaac cgcctggcaa
     5221 caaccttcat gattgtgatt tcccgacatg ttgctattcc cgatggtgag cttgagatca
     5281 ccgccattcg tgcgcagggc gcgggcgggc agcatgttaa taagacctca acggctattc
     5341 atctgcgttt tgacattcgg gcgtccagcc tgccagagta ttacaaagag cgtctgctcg
     5401 ccgccagcca tcatttgatc agcatgatgg cgtaattgtc attaaggcac aggaataccg
     5461 cagtcaggaa ctgaaccgcg aagcggcgct ggcccggctg gtggcagtga ttaaagattt
     5521 aacaacagaa caaaaagccc gacgacccac gcggcccacc cgtgcatcga aagagcgcag
     5581 gctggcatag aaagcacaaa aatcaagcgt gaaggcgatg cgcggcaaag tacgcagcgg
     5641 tcgggaataa aaagaaggaa tggatggtga aaaaagcgat agtgacagcg atggctgtaa
     5701 tcagcctctt tactctgatg ggatgtaata atcgggccga agtcgatacg ctttctccgg
     5761 cgcaggctgc cgaactaaaa ccgatgccgc aaagttggcg cggcgtgctg ccgtgtgccg
     5821 attgcgaagg gatcgaaacc tctctgttcc tcgaaaaaga cggaacatgg gtgatgaatg
     5881 agcgttatct cggtgctcgt gaagaacctt cctccttcgc ttcctacggt acatgggcgc
     5941 gaaccgctga caagctggta ttaaccgaca gcaaaggtga aaagtcatat tatcgggcga
     6001 aaggcgatgc gctggagatg ctcgatcgtg aaggcaatcc aattgaatcg cagttcaact
     6061 atacgctgga accggcacaa tccagtttac ctatgacgcc gatgaccctg cgaggcatgt
     6121 atttttatat ggctgatgcg gcgaccttca ctgattgcgc gaccggaaaa cgtttcatgg
     6181 tagcgaataa cgcagagctg gagcgtagct acctggctgc gcgcggtcac agtgaaaaac
     6241 cggtgttact gtcagtagaa ggtcacttta cgcttgaggc taatccggat actggtgcgc
     6301 caaccaaagt attagcgccc gatacggcgg gtaaatttta cccgaataag gattgcagta
     6361 gtttggggca gtaaacctgc ttaaggcaga aacaaacgca aaattgcctg atgcgctgcg
     6421 cttatcaggc ctacagcata taatgcaaca ttttgaatta gcacgatttt gtaggccgga
     6481 taaggcgttc acgacgcatc cggcattttg tgcacgaggc tggctttatc actgagtcag
     6541 cccaacaaag cgacccgcct tgatataaat ccctggcttc aggtgcccaa catactgtaa
     6601 ctgcgtttct ggcttaaacg cagagacatc gccagtacgc atatgcatca gatcggcagg
     6661 gctaatccag cctgattgcg ccagcgtaag cggatgcccg gctttggcaa atgcatcagt
     6721 gacaaattcc gaacaaaacc acgacttttt gtctccttcg cccacactgc ttaactgcgc
     6781 tttcgccagg ccgctgacgc actgttggcg aaaatcctcg gagaacggat tcagtgagca
     6841 catctggcga gtcaccataa agggaataaa ttcgacaatg ccgcgatagt tataactgct
     6901 atctttgatt ttattggcaa acgcggtgat atctgtggct tgttgcgggg taagatccgg
     6961 gactcgtaag acgaaaagct tatcactatg cttcatcgct tttttaaggg aaacgatctg
     7021 gacgccagcg cctgtcgctt ctgcaacgtt attatcaccc agatagattg caacgtgact
     7081 cacagaggaa gtgctgaaga cgcggattcc aaatgaggtt accccaaggc ttgaggagaa
     7141 cagcaaatcg ccgggtttga gatcaggtac cgttatttct tttattgatt gttcggtgaa
     7201 agagctttga tgctggaatt tgacagccca tgtttttgcc tcggcatcta ctgctgtggc
     7261 tgatgggtct ggctggctga tatcaacggt acaagccgaa agtaaaagaa agctggggag
     7321 aagcaggcgg cagtacgcct ttggtttatc cattttatac aatccatgta aaaaagggcc
     7381 ctgaaattca ggaccctttc tggcatcagc ctttaatctg tttcaccaga tattcgacga
     7441 tgtcaccagt cttaattaac tgtttctcgc cgttgcgacg atatttatat tcgatatcgt
     7501 cgttgtcgag gttacggtcg cccagcacaa tagtgtgcgg aataccgatc agttccatat
     7561 cagcaaacat cacgcccgga cgctctttgc ggtcatccag cagcacttcg ataccttgtg
     7621 cacgcagttc gctgtacagt ttctcagcaa gctcttgtac gcggaaggat ttgtgcatgt
     7681 tcatcggcag aatcgccacc tggaacggcg cgatagcgtc aggccatacg atgccgcgtt
     7741 cgtcgtagtt ctgctcaatc gccgcagcta ccacacgcgt taccccgata ccgtagcaac
     7801 ccatcgtcag gatttggtta cggccatctt caccctgtac ggaggctttc agtgcttcgg
     7861 agtacttggt acccagctgg aagatgtgac caacttcgat accacgtttg atcagcagcg
     7921 taccctggcc atccgggctt ggatcgccag ccaccacgtt acggatatct gcaacttccg
     7981 gggtagcgac atcgcgatcc cagttgatac cgaagtagtg tttaccatcg atgttagcac
     8041 cagcagcgaa atcactcatc gccgcaacgg tacggtcaat caccaccgga atcggcatgt
     8101 ttaccggacc cagtgaaccc ggaccggctt taaccacagc acgaatttct tcttcggtcg
     8161 cgaaagtcag cgggcttgca acctgcggca gtttttctgc tttaacttcg ttcagctcgt
     8221 gatcaccgcg caccagcagc gcaaccagcg ggaagctgct gccttcaacc gctttaacca
     8281 gcagagtctt aaccgttttc tcaatcggca gattgaactg ttcaaccagt tccgcgatgg
     8341 ttttcgcgtt cggcgtatca accagcgtca tttcctgggt agcagcagcg cgcggttctt
     8401 tcggcgcgat agcttctgcc agttcaatgt tcgctgcata gtcagaggtg tcggagaaga
     8461 ccacatcgtc ttcaccgctc tgcgccagca cctggaattc gtgagaggcg ctgccgccga
     8521 tagaaccggt gtcggcttgc acggcgcgga aatccagccc catgcggctg aagattttgc
     8581 tgtaggccgc atacattgca tcgtaggttt cctgcaggga ttcctgagaa gtatggaaag
     8641 agtaagcatc tttcatcagg aattcgcggg aacgcatgac gccgaaacgc ggacgcactt
     8701 cgtcgcggaa cttggtctgg atctgataga agttcagcgg cagctgtttg taagagctaa
     8761 gctcgttacg aatcaggtca gtgataactt cttcatgagt tgggccgagt acgaacggac
     8821 gctcgccacg gtcaacaaaa cgcagcagtt ccggaccgta ctgttcccaa cgaccactct
     8881 cttgccacaa atcggctggc tgaaccaccg gcatcgacac ctcgattgca ccggcgttgt
     8941 tcatctcttc acgcacgatg ttttcgactt ttttcagaac gcgcacgccg gtcggcagcc
     9001 aggtatataa cccggaggcc agcttgcgga tcatcccggc gcgcagcatc agctgatggc
     9061 tgatcacctc ggcgtcggca ggtgtctcct tgagagtgga gagcaggtat tggctagtac
     9121 gcatgttgtt acggttccag ttggaaggta gaacaggctc aaggcgagcc tgggacaaaa
     9181 aaagtgattt agtttaccag tgcaaaagaa atgtcaaaag agaagggcgt gaatttaacg
     9241 cggttccagc gcaaagactt caaaacctgc gtcggtgacg cgccagcgaa cgttaaaatc
     9301 atgtagccag acggcatagg ttttgcccgt ttcctcacct ttacgatagg ccgggcgcgg
     9361 gtcctgcgcc agtacttcgc ggataaacag cgttaactgc ggataacgct tctccagcgt
     9421 caaaagctgc ttttcgacct ctgcggtaaa actcaccgcc atctctgcag ctggcgcact
     9481 ttgcgcatag ctggcactgg catcgggaag cgattcggca aaggggagat acggtttgat
     9541 atccactacc ggcgtaccat cgaccagatc cagactgccg agcttcagaa tcacgctgtc
     9601 tttatggcaa acaacctctt tcagctctac cagcgacata ccaattgggt tagggcggaa
     9661 agtagagcgt gttgcgaaaa cccccattct ggcgttaccg ccgaggcgcg gtggacgcac
     9721 agtcggacgc cagccgcctt ccatcgtttg atgaaagacg aaaaggatcc ataaatggct
     9781 gaacgcttcc aggccgcgaa cggcgtcggc ctggttgtag ggagcaatga gatgcagttc
     9841 gccgttggcg ctttttacca gaccaggctg gcgcggaacg gcgaattttt ctttataggg
     9901 cgagcgaata acgcctattt gctcgaactg gaaactgctc atttcgccgt aatgttaagc
     9961 gcagagccga tacatacagc ctgacgatag cagcctagcg taccgctggt gacttcgcag
    10021 ctatgcagta ataccgcatt ggctttcatt ttagaggcgt tgatttgcat ccgcttacgc
    10081 gcggttggaa tgctcggcgg agagtcctga ttagaggcct ggcaagagtc gccactgact
    10141 tcaccgagat cgcggaacgg tttgccgact aattcttctg cattggtata aattcggacc
    10201 ggcgtggcgc gcggcgcttt cggttttgca ggctccgctt tcggctgggg tgcagtgctt
    10261 tgaacgggtt cgacagggga tctgcttaac atggaacagc cgcttagcat gagtgctact
    10321 aaacagatcg gtaaagcacg catagtattt cctcaatgta tgatcaaaac gtcaatattg
    10381 aatcaggagc ttgtaaaaat gacaagacgg gcaagcgccc gtcctgaatg atattacaaa
    10441 ttgtggaaac agcctaaaaa ttaccagcct ttaacagcgc cgccgttaaa cactttgttt
    10501 gctgcttcgt aaacttcatc agactgataa gcctggacga atttcttcac gttctcggcg
    10561 tctttgttat cttcacgcgt tacgatcagg ttcacgtacg gggagtcttt atcttcgaca
    10621 aagataccgt ctttcgctgg agtcaggcca atctggctgg cataggtggt attgataact
    10681 gccagagcga tttgcgcgtc gtccagagat cgaggcagct gcggtgcttc cagctcaaca
    10741 attttcagat tttttgggtt ctcaacaaca tcaagaacgg tcggcagcag gccaacgcca
    10801 tctttcagtt tgatcaagcc cactttttgc agcagcagca gtgaacgacc aagattagtt
    10861 ggatcgtttg gcacggcaac ctgcgaacca tcctgcagtt catccagtga tttgattttc
    10921 ttggagtaac cagcaatcgg ataaacaaaa gtgttgccta ctgcgaccag tttgtagcca
    10981 cgatctttca gttgctgatc aaggtacggt ttatgctgga aggcgttggc gtcgatatcg
    11041 cctttgctca atgcttcgtt tggcagaaca tagtcgttga aggttaccag ctcaacgtcc
    11101 aggccatatt tgtctttcgc aactttctgc gcgacttctg ctacctgctg ttcggcacca
    11161 acaatcacgc cgactttaat gtggtttgga tctttttcat cctgaccgca gcctaccagt
    11221 gccagtgatc caatcagggc tcccactgcc gcaaaggttt tgaatttgaa cgccatacct
    11281 tattccttct tcaattattt atgttgtgtt gaacgttact tgcgagtgac agcccggacg
    11341 atgcggtcgc ctgcgaactg aattaaataa accagaatga ccagcaatac cagtaccgta
    11401 ttcatcaccg tcgcgttgta gccgatgtag ccatactgat agccaatctg acctaaacca
    11461 ccggcaccga ctgcaccacc catcgcggaa taaccgacca gggtaatcag ggtgatagtt
    11521 gccgcattca ccagacccgg caacgcttcc ggtaacagca ctttacggac gatctgcatt
    11581 ggcgtggcac ccattgcgcg ggaagcttca attaacccgg ttgggatctc cagcagagcg
    11641 ttctcgacca tacgggcaat aaacggtgct gcaccaacgg ttaacggaac aattgctgcc
    11701 tgcaaaccaa tcgatgtacc gacaataacg cgggtaaacg gaatcatcca taccagcaag
    11761 ataatgaacg ggatggaacg gaaaatgttc acaatcgcag aaacggtacg atacagcttc
    11821 gcgttagcaa taatttgccc cggacgcgtg acataaagca gaacgccaac cggcagacca
    11881 atcacaaagc caaaaaatcc ggatacgaag gtcattgcca gcgtttccca tacgccacga
    11941 accagcagcc acatcatcgg ctcagacata acccagtacc tctactttta catggtgttc
    12001 ctgcagccag gcaatggcgg cttgcgtatc ttgttgtgtg ccgtgcattt cagtcagcat
    12061 gatgccgaat ttaacgccac cggcgtagtc catctgcgcg ctaataatgt tgttgttgac
    12121 gttgaaacga cgcgcggttt cagaaagcag tggggcatcg accgattgac cggtaaactc
    12181 cagacgcagc atcggcacgc agtcagtaaa tggctccgct tgcagacgtt cctggtaatc
    12241 ttccgggata tccagatgca gggtcgactg aataaacttc tgcgccagcg gcgttttcgg
    12301 atgcgagaac acttcactta ccgtgtcctg ctcgatcagt tcgccattgc tgatgaccgc
    12361 cacgcaatca caaatacgct tcacaacgtc catttcgtgg gtgatcaaca gaatcgtcaa
    12421 acccagacgg cggttaatgt ctttcagcag ttcgagaata gaacgtgtcg ttgccgggtc
    12481 cagcgcgctg gtggcttcat cacacagcaa tactttggga ttgctggcta atgcacgggc
    12541 aatcgccaca cgttgtttct gcccaccgga aagattcgac gggtagctat catgcttatc
    12601 gccaagacca accaatgaca gcaattccgt cacgcgacgt ttgatctcgt ctttcggtgt
    12661 gttgtccagc tccagcggca gagccacgtt gccaaaaaca gtacgcgaag agagcaggtt
    12721 aaaatgctgg aaaatcatac caatctggcg gcgagctttg gtcaactcgg attctgacag
    12781 cgtggtcaat tcttggccat cgaccagcac gctaccctcg gttgggcgct ccagcaggtt
    12841 tacacaacgt ataagcgtac tcttacccgc gcctgaggca ccgataacgc cataaatttg
    12901 tccagctggc acatgcaggc tgacgttgtt caacgcctgg atggtgcggg tgccctggtg
    12961 gaacactttg gtgatattcg aaagttttat cattgattat ttattatcgt cattaagtta
    13021 gtcgtggcat ctcgaatgcc tgaaacgggc aacgccgtca atgaaatgga tgttaaggca
    13081 tccagacgtc taaatcaatc aggtttatgc gaagagcact ttcttgcagg tcgaaacatg
    13141 cgatactagc gtcccatgcc ttattaagga gctataaaag gtggcgaaga gcgtacccgc
    13201 aatttttctt gaccgtgatg gcaccattaa tgtcgatcac ggctatgtcc atgagatcga
    13261 caactttgaa tttatcgacg gtgttattga cgccatgcgc gagctaaaaa aaatgggctt
    13321 tgcgctggtg gtagtaacca accagtctgg cattgctcgc ggtaaattta ccgaagcaca
    13381 gtttgaaacg ctgaccgagt ggatggactg gtcgctggcg gaccgagatg tcgatctgga
    13441 tggtatctat tattgcccgc atcatccgca gggtagtgtt gaagagtttc gccaggtctg
    13501 cgattgccgc aaaccacatc cggggatgct tttgtcagca cgcgattatt tgcatattga
    13561 tatggccgct tcttatatgg tgggcgataa attagaagat atgcaggcag cggttgcggc
    13621 gaacgtggga acaaaagtgc tggtgcgtac gggtaaacct attacacctg aagcagaaaa
    13681 cgcggcagat tgggtgttaa atagcctggc agacctgccg caagcgataa aaaagcagca
    13741 aaaaccggcg caatgattaa aagatgagcg gttgaaataa aaatgcattt ttccgcttgt
    13801 cttcctgagc cgactcccta taatgcgcct ccatcgacac ggcggatgtg aatcacttca
    13861 cacaaacagc cggttcggtt gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    13921 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    13981 tttaacaatt tatcagacaa t
//
LOCUS       DAEEKQ010000102        13903 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.102, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000102 DAEEKQ010000000
VERSION     DAEEKQ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13903
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.102"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            242..1495
                     /gene="kch"
                     /locus_tag="KLK57_002820"
     CDS             242..1495
                     /gene="kch"
                     /locus_tag="KLK57_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HBE0893137.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISIIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(1550..1723)
                     /locus_tag="KLK57_002821"
     CDS             complement(1550..1723)
                     /locus_tag="KLK57_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HBE0893138.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            1866..3326
                     /gene="cls"
                     /locus_tag="KLK57_002822"
     CDS             1866..3326
                     /gene="cls"
                     /locus_tag="KLK57_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HBE0893139.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            3361..3690
                     /locus_tag="KLK57_002823"
     CDS             3361..3690
                     /locus_tag="KLK57_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HBE0893140.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDIFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="KLK57_002824"
     CDS             complement(3743..4747)
                     /gene="oppF"
                     /locus_tag="KLK57_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HBE0893141.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATGGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPISALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTCCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="KLK57_002825"
     CDS             complement(4744..5757)
                     /gene="oppD"
                     /locus_tag="KLK57_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HBE0893142.1"
                     /translation="MSVIETATVPLAQQQADVLLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="KLK57_002826"
     CDS             complement(5769..6677)
                     /gene="oppC"
                     /locus_tag="KLK57_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HBE0893143.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="KLK57_002827"
     CDS             complement(6692..7612)
                     /gene="oppB"
                     /locus_tag="KLK57_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HBE0893144.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIVALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="KLK57_002828"
     CDS             complement(7698..9329)
                     /gene="oppA"
                     /locus_tag="KLK57_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HBE0893145.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDSTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            9613..9909
                     /locus_tag="KLK57_002829"
     CDS             9613..9909
                     /locus_tag="KLK57_002829"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893146.1"
                     /translation="MRFLLANTFCKIVKQFSFIDRIRIYSLHPCLNKHLSIQSNRDWL
                     SMSIFSDYSSSSEMHNNLTIDYYLALSSTKGSGITNIISIILQQAQDYDVAKIT"
     gene            complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="KLK57_002830"
     CDS             complement(10067..10714)
                     /gene="ychE"
                     /locus_tag="KLK57_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HBE0893147.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            11192..13867
                     /gene="adhE"
                     /locus_tag="KLK57_002831"
                     /gene_synonym="adhC"
     CDS             11192..13867
                     /gene="adhE"
                     /locus_tag="KLK57_002831"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HBE0893148.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
BASE COUNT         3659 a         3437 c         3229 g         3578 t
ORIGIN      
        1 cttatagccg gtcagtgcat tcactaccac aagcagataa taactttatc tgtatttctt
       61 tatatgatgt gagcatattc aggaaaaatg acctttcctg aggtacagtt gctcgctcaa
      121 atcctttgat tactgcggat aaagtcatct actattgttt aacacattca cgcgctgcat
      181 gtatgttcag aaaatgtgca cgctaagata attaaacaaa attgtggtta cagaggtatc
      241 agtgagtcac tgggctacat tcaaacaaac agcaacaaat ttatgggtga cccttcggca
      301 cgacattctc gcgctggccg tctttttaaa tggattgctt atttttaaaa caatctatgg
      361 tatgtcggtc aatttgcttg atattttcca tatcaaagca ttttcagagt tggatctctc
      421 cttgctggca aacgccccac tatttatgct cggcgtcttt cttgtcctga actccattgg
      481 cttactgttc cgggcaaagc tcgcatgggc aatcagtatc attttgttgt tgatagcgct
      541 aatttacacc ctgcattttt atccctggct gaaatttagt attggatttt gcatttttac
      601 gctggtgttt ttgctgatac tgcgcaaaga cttctcccac agtagcgccg cagccgggac
      661 aatttttgca tttattagtt tcacgacatt actgttttac tcaacctacg gtgcgcttta
      721 tttaagcgaa ggttttaatc cgcgaataga aagtttgatg accgcgttct atttttcgat
      781 agaaaccatg tcaaccgtcg gctatggcga tattgtccct gtttctgaat cagcacgatt
      841 gttcactatt tcgatcatta tttccggcat taccgttttt gccacatcta tgacctcaat
      901 ttttggtccg cttatccgcg ggggattcaa caaacttgta aaaggaaaca atcatacaat
      961 gcatcgtaaa gatcatttta ttgtttgcgg acattcgatt ctcgccatca atacgatcct
     1021 gcaactgaat caacgcggac aaaacgtaac ggttatcagc aacttgcctg aagatgatat
     1081 caagcaactt gagcaacgct taggcgataa cgctgatgtt atccccggtg acagtaatga
     1141 cagttcagta ttaaagaaag cgggaatcga tcgttgccgg gccattctgg cgctgagtga
     1201 taacgatgca gataacgcgt ttgttgtact ctcggcaaaa gatatgagca gtgatgtcaa
     1261 aacagttctc gccgtcagtg atagcaaaaa cctgaataag attaagatgg tacatccgga
     1321 tatcattctc tcaccgcaac tgtttggcag cgaaattctg gcgcgagttt taaatggtga
     1381 agagattaat aatgatatgc tcgtttcaat gttgttgaac tccggtcatg gcattttcag
     1441 cgataacgat gaacaagaaa cgaaagctga cagtaaagaa tcagtgcaaa aatagagttt
     1501 aaagacgaaa gagtaaactt attgggcacc gcaatggtgc ccagtatatt tagatattgt
     1561 agatcgcaaa caataacgag ttacgctgtt tgtttagaat gcacttcctg atgctgtgga
     1621 tacgcatatt tcggcgcgat tgcccctcaa gccaacgcga tttacgtcgg ctagcctgac
     1681 gctgcatgcg ccagcgcccc acttccgttc tactccgctt catgttaact actctatgca
     1741 ataacagaat ggtcattata cccccacccc cgacgctgac cagtgctttt cccgcgtttt
     1801 tatttgccag atgaatccat atgcgtaaac tcataacaat gcgctttcaa aaggatttct
     1861 aatctatgac aaccgtttat acgttggtga gttggttggc cattctggga tactggttgc
     1921 tcattgcagg cgtaacttta cgcattctaa tgaaacgacg cgcagttccc tccgcgatgg
     1981 cctggctgtt gattatttac attctgccgt tagtcggaat tattgcctat cttgccgttg
     2041 gcgagctcca tttaggcaaa cgccgcgctg agcgcgccag agcgatgtgg ccttccaccg
     2101 caaaatggct taacgacctt aaagcctgta agcatatctt cgccgaagaa aatagcagtg
     2161 tcgctgcgcc attattcaag ctttgcgagc gtcgtcaggg gatcgctggg gtcaaaggga
     2221 atcagctaca actgatgacc gagtcagatg atgtgatgca ggcgttaatc cgcgacatcc
     2281 agctcgcgcg ccataatatt gagatggtgt tttatatctg gcagcccggc ggcatggcgg
     2341 accaggtggc tgaatcatta atggcggctg cacggcgtgg tattcattgc cgattgatgc
     2401 tcgactccgc cgggagtgtg gcttttttcc gcagcccgtg gcccgagcta atgcgtaatg
     2461 ccggtattga agtggtcgaa gccttaaagg tcaatctgat gcgtgtgttt ttacgccgta
     2521 tggacctgcg ccaacatcgc aagatgatca tgatcgataa ttacatcgcg tacaccggca
     2581 gcatgaatat ggtcgatcct cgctacttca aacaagatgc gggcgtaggg caatggattg
     2641 atctgatggc gcgtatggaa ggccccatcg ccaccgcgat ggggattatt tattcctgcg
     2701 actgggagat tgaaaccgga aaacgtattc tgccgccacc accagatgtc aatattatgc
     2761 cgtttgaaca ggccagcggt cacaccattc acacaattgc ttctggcccc ggctttccgg
     2821 aagatctcat tcaccaggca ttattgactg cggcttattc ggcgcgtgaa tatttgatca
     2881 tgacaacgcc ctactttgtg ccaagcgatg atttacttca tgcgatttgc acggcggcgc
     2941 agcgcggggt ggatgtcagt attatccttc cgcgaaaaaa tgactcgatg ctggtcggct
     3001 gggccagtcg cgcattcttt acggaactgc ttgctgctgg ggttaaaatt tatcagtttg
     3061 aaggcgggtt actgcatacc aagagcgtgc tggtcgatgg cgaactaagt ctggttggca
     3121 ccgttaacct tgatatgcgt agtctgtggc tgaatttcga gattaccctg gcaatcgacg
     3181 ataaaggttt tggtgcagac cttgccgccg ttcaggacga ttatatttcg cgttcacgtc
     3241 tgctcgatgc ccgtttatgg ctaaaacgtc cattatggca acgtgtcgcc gagcgactgt
     3301 tttacttctt cagcccgttg ctgtaaaacg tcgccaacag aggttaaaca ggtagtgact
     3361 atggatatgg atctaaacaa tcgcctgact gaagatgaaa cgcttgagca ggcctacgat
     3421 atttttctcg aactggctgc cgacaatctc gacccagctg atgttctgct gttcaatctt
     3481 cagtttgaag agcgcggcgg cgcggagtta ttcgatccgg cggaagactg gcaggaacat
     3541 gttgatttcg atttgaaccc ggactttttt gccgaagtgg tgattggtct ggcggacagt
     3601 gaggatggtg aaatcaacga tattttcgcc cgcattctgt tatgccgcga aaaagatcac
     3661 aaactttgcc atatcatctg gcgggaataa ataatcgctt tcggcgtgaa aaagggctga
     3721 caactgtcag cccttattgt gtttaaagcg gatcgacttt caggcaagaa acggcgtgtc
     3781 tgaaactccc ttccagcaca ggacgtgttt tggcgcactc cggaccggca atcgggcaac
     3841 aggtacggaa aacacaaccg gaaggcgggt tgatcggcga cggtaattcc ccttccagta
     3901 actggatggt tttgttcttc tccagatccg gatcaggtat ggggactgcc gacatcaatg
     3961 ccctggtgta aggatgtagt ggattgtggt agacctcatc ataggtcccc agttctaccg
     4021 catggccgag atacatcacc aacacacgat cggaaatgtg ttttaccacg gccaggtcat
     4081 gagcgataaa aattaatgac aatcccatct cacgttgcag ctgctggagc aggttgacca
     4141 cctgcgcctg aattgacacg tccagcgccg aaatcggctc atcgcagata atcagcttcg
     4201 gttcaagaat aagagcacga gcaatcccga tacgctggca ctgcccaccg gagaactcat
     4261 gcggatagcg gttaatcagg ttaggcaata acccgacttt cagcatcatc gccttcacgc
     4321 gctcgcgaac ttcctggcgt gacattttcg gatgataagt acgcagtggt tcagcgatga
     4381 tctcgccgat ggtcatacgc gggtttagcg atgccaacgg atcctggaaa atcatctgaa
     4441 tatcactgcg aacggcacgc cattcatcgg gcttcatgcc cagcaactct ttacctaacc
     4501 aggcaacatg accgccggtc gctttaacca ggccaatgat ggcgcgagca aaggtggact
     4561 taccgcatcc cgattcccct accacaccta atgtttcccc ttcatacagg cgaagcgtga
     4621 caccatcgac ggctttgaga gttttcggcg gttgccagaa ccactgtttg ccatctttga
     4681 tttcaaagtg cactttcaga tcggcgattt cgaggaggac ttttcttcct tcggttacag
     4741 cattcataac agttcctcca ccggtttaaa gcaagcacgc agacggccag gcgtaaactc
     4801 ttccagcggc ggtgcgctac tacaaatttc catcgcatgc ggacaacgtg gctggaacgg
     4861 gcaacctttc ggtaatcgca gcaggtttgg cggattacca gggatggtca acattgtttc
     4921 accttccgca tcgagacgcg gcaccgcgtt gagcaaaccg atagaataag gatgaacggg
     4981 ttgataaaag acatcgcgcg cgttgccata ttccatcgtg cgcccggcgt acattaccag
     5041 cactttgtcg cagatccccg ccaccacgcc aagatcgtgg gtaatcataa tgatggcggt
     5101 attaaattcc cgcttcagtt catttaatag cgtcatgatc tgcgcctgta cggtgacgtc
     5161 cagcgcagta gtgggttcat ccgcaatcag cagcttaggt cgacatagca atgccatcgc
     5221 aatcataact cgctgacgca tgccaccaga aaattcgtgc gggtacattt tcatgcgttt
     5281 acgtgcttcc ggcattttta ccgcatcgag catccgcacc gactcttcaa acgcctcagc
     5341 tttgctcatg ttcttatgca gcatcagcac ttccatcaac tgctcaccga cgcgcatata
     5401 gggattcaac gaagtcattg ggtcctgaaa aatcattgag atttgttcag cgcgcagttt
     5461 attgagttca cgctctggca aattgaggat ttcacgccca ttgaaggttg ccgatccgcc
     5521 aatacgtccg ttggcagcca acaggcccat caacgcaaat gcagtttgcg atttacccga
     5581 accagactca cctacaatgc ccagcgtttc tccggcacgt agagaaaaat tcaaatcatt
     5641 gaccgccgtg acatcgccgt ccggggtact aaaggtgaca cgcaaatctt tcacgttcag
     5701 cagtacgtca gcctgttgtt gtgcgagcgg cacagttgca gtttcaatta cgctcatggc
     5761 tgcactcctt aacgatcttt cgggtcgaga gcatcacgca agccatcgcc gataaagttg
     5821 aaacaaaaca gcgtcaccac gaggaatccc gctgggaaca acaataacca tggagagact
     5881 tccatcgagt tcgcgccatc actcagcaat gcaccccagc tgcttaacgg ctcttgcgta
     5941 cccaacccca ggaagctaag gaaagattca aagaggatca tgctgggcac cagaagtgat
     6001 gcgtagacca ccaccacacc gagtacattc ggcacaatgt ggcgaataac aatgcccgat
     6061 gtcgatacac cgccaacttg tgccgcctca ataaactctt tgcgcttcag actcagggtt
     6121 tgtccacgca caatacgagc catatccagc caggaaacca tgccaatcgc cacgaaaatc
     6181 agcaggatgt tttgaccgaa aaaggtcaca agcaaaatga cgaagaacat gaatgggaag
     6241 gagttgagga tttccagcag acgcatcatt accgaatcca ctttaccgcc cagataaccg
     6301 gaaagcgaac cgtaaagtgt ccccacgacc actgccacca gtgccgcagc aacaccgacc
     6361 atgagtgaga tacgcccgcc aatcgcaacg cgcacaagca ggtcgcgacc ggatgagtca
     6421 gtaccaaagt agtgaccgga ctccatatcc ggggcgctgg acatcatcgc ccagtcagta
     6481 tcgtcatagg caaactgcga aagcatcggt gccaggatta caaataacgc gatcagcacc
     6541 agcactatca gactggcaac cgccgcacgg ttatgcataa aacgtcgacg tgcgtcctgc
     6601 cacaagctgc gcccttcgac ctccagcttt tcactgaaat tttccagcgt ctcgctgttt
     6661 ttcttactta acatcatcgc gagctccagt atcagtaacg gattttcggg tcgataaccg
     6721 catatagcac atcgacaatg gcattaaaca aaatggttaa agcaccaacc aggatggtca
     6781 ggcttaacac taaggaatag tcacggttca atgcgccatt aacgaacagt tgtccaatcc
     6841 ccggcaaacc ataaatggtt tcgataacca tagaaccggt aataatgccg acaaatgcgg
     6901 ggcccatata agagagcacg ggtaacagag caggttttaa tgcgtggcgt aaaatgatcc
     6961 gccgcatagg taaccctttc gcccgggcag tacgaataaa gttggagtgt aatacttcaa
     7021 tcatagagcc tcgggtaata cgcgcaatac tggcgatata agcgagtgac aacgccacca
     7081 tcggcaatat catgaattta agcgccccac cattccagcc accgcccggc agccaatgca
     7141 aaatgatcgc aaatatcatg actaataatg gcgcaaccac aaaactgggg ataacaaccc
     7201 cggtcattgc cagccccatc acggtatagt cccatttggt gttttgtttt aatgcgacaa
     7261 taacgccagc actaacaccc agtattaccg caaggaaaaa tgcggcggct cccagtttgg
     7321 cagaaacggg aaaactggat gcaaccaggt cattgaccga ataatcttta tatttaaacg
     7381 atggaccgaa atcaccgtgc gccagttgtt tcaggtagct gaaatactgt gtcatgatcg
     7441 gatcattaag atgatatttc gcttcgatat tggccatcac ttccggcggt aaagtacgtt
     7501 cgccggtaaa agggcttccc ggcgcgaggc gcatcataaa gaacgaaata gtaataagaa
     7561 taaatagcgt cggaatcgct tccagacagc gacgtagaat aaattttaac attgccctac
     7621 cttctggcct gtgccttctg taatgcgata aaaatcagac accgtggagc aggacactcc
     7681 tgccccacgt attgccatta gtgcttcaca atgtacatat tccgggtgta ggtattatcc
     7741 agcggatctt tgccggtata gccaccaacc cacggtttca ccagacgcgc attcacgtag
     7801 taataaacag gaacaatggc cgaatcctta tccagctgtt gttctgcttt agtgtacaga
     7861 gctgtgcgct gcgcctcgtc agtcactttc agcgtttccg ccataatgct gtcaaaggcc
     7921 gggctcttat aatgcgcggt attcatcgag ctgttcgaca gcatggtgtt caggaaggaa
     7981 gttggttcgt tgtagtctgc acaccagcct gcacgggcca catcaaaagt gccctggtga
     8041 cgggtgtcga ggaacgtttt ccactcctgg ttaaccagtt tgacgtttac accaatgttt
     8101 ttcttccaca atgaagaggc agcaatcgcc agctttttat gcagatcaga ggtgttatac
     8161 agcaggttga tggtcaacgg tttgtctgcg gtataacccg cttcagccag cagttttttc
     8221 gcttcttcgt tacgtttttc ctggctccag ccaaaccatt ctggctgagt caattttgcg
     8281 ccatcagtat acggtggagt gtaaccatag gcgggcatgt cgccctgcgc tttcacttta
     8341 ttaacaatga tatcgcggtc catacctagt ttcagtgcgg tacgcacacg cacatcgttg
     8401 aatggcggtt tctggttgtt aatttcgtaa tagtaagtgc acaggtatgg atcaacgtga
     8461 acttcgtccg ggatctcttt tttcagcttc tggaacaatt cgatcggcat gttgttatac
     8521 gtcatgtcga tttcaccact acggtagcgg ttgacatcgg taacttcaga agcaataggc
     8581 aaataggtta cctggttaat aacggttttc gcgttgttcc agtaggtcgg gctgcgttca
     8641 agaacgattc gttcgtttac gacccaatct tttaaggtat aggcaccgtt ggtgacgata
     8701 ttaccaggct gggtccattt ttcgccgaat ttctcgatag cggcttttgg caccggtgaa
     8761 gttgatgggt gaacaagtaa tttatagaag tacggaacgg gttcacttaa ggtgacttct
     8821 aatgtgtgat catcaatagc tttcacgccg agatcggtaa tcggtttttt cccttcaaga
     8881 atttcatcaa taccggcgat atgcccatat tgcagataac tggcatacgg agaagcagtg
     8941 ttcggatcaa cagaacgttg ccagctatac acaaagtctt gtgctgtgac tggcgtgcta
     9001 tcagaccatt tcgcatcttt acgcaaatgg aaggtccaga ctttcgcgtc tttattatcc
     9061 caggattcag cgacgccagg tgctggatga ccgtcaagat cgctgaccag taagccttca
     9121 aacaggtctc ggctgatatt agactccgga acaccttcaa ttttgtgcgg atctaatgac
     9181 tgaacttctg aaccattgtt acgtaccagt gtttgttttt ccgccagtgt gacgcctgcg
     9241 ggtacatcag ctgccagcgc gacattccct gccattagcg cagccagaac gccagctgct
     9301 actaaacttc tcttggtgat gttggtcatt gttttttgga ctccctcatt ataattactg
     9361 gtattaacca gcatgtgtaa tcccggattg ggaccctgca caggctcgga gactttctgc
     9421 tgtcaggtta ctttatcgtt acgtattact atcaccgact ttatttattc tggtgattca
     9481 gacgatcacc ttatgtcgat tcgtactgtg tctcccctgt cgagacacat tattctttcg
     9541 cgacgctctt taattgttaa ttattctcat taccaataaa gaatcgtctg gcggttgccc
     9601 ggaaagtacc aaatgcggtt cttactcgcc aatacatttt gcaaaattgt taagcaattc
     9661 tcttttattg accgtatcag aatctatagc ttgcatccat gccttaacaa acatctttca
     9721 attcaatcaa atagagattg gctaagcatg tctatctttt ctgattacag ttccagtagt
     9781 gaaatgcaca ataatttaac aattgattat tatttagcac tttcctctac caaaggcagt
     9841 ggaattacta atatcatttc aattattctt cagcaagccc aggattatga tgtggcgaaa
     9901 ataacatgat ggtgaggaga agcagttaag gaaatcctta taactaactg taatttgaga
     9961 agaatatttt tacctattcc ttttgctttt gataactgcg cgggaacaaa gtaaaaataa
    10021 tgacgaagta aacggagcat ggcagctccg tttcatttga aaggaattaa ttaagcaggc
    10081 cggggaaaat ccccttaata ccagtaacga taaattcaat ccccaatgcc atcagcaata
    10141 gccccataat acgcgtaatc acgttgatgc cggtctggcg taaaacccgt accagccacg
    10201 gtgccatgcg gaacaatccc cagcaacata aagcgaacaa tgcaatagcc acaaagaaac
    10261 caaacagata gctaatgctg tgataacgcg taccccagac gatggtagaa ctgatcgccc
    10321 ctggccccgc catcaacggc aacgccaatg gcaccacacc aatgctttca cgtaccgcgg
    10381 tttctgattt ttcttgcttg ttctgtttat cctcgccaag cttgccgctg atcatcgaca
    10441 tcgctatcgt caccaccagg atgccccctg cgatacggaa cgaatcaatt gatataccaa
    10501 aaagttgcag aatcgtgtcg ccgagaaaaa gcgagatcca caagataatg gccacagaca
    10561 ggttggctgt aaggttagtt ttgtttcgcg ctgccgctgt ctgataactg gtcatgctga
    10621 taaagacggg aataatccct accgggttga ccagcgcaaa taacccgatg aaaaatttga
    10681 agtaaacggg aaaatcaaaa aaggtctgaa tcacggttag ctccgaagca aaagccggat
    10741 aatgttagcc ataaattaag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
    10801 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
    10861 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
    10921 aagcgatgct gaaaggtgtc agcttagcaa aaatttgatt tggatcacgt aatcagtacc
    10981 cagaagtgag taatcttgct tacgccacct agaagtgacg cattagagat aataactcta
    11041 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
    11101 ataggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
    11161 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
    11221 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
    11281 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
    11341 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
    11401 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
    11461 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
    11521 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gtctgaagac
    11581 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgccacca acaaagcggc
    11641 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
    11701 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
    11761 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
    11821 cggtgtaggc gcgggcaaca ctccagttgt tatcgatgaa actgctgata tcaaacgtgc
    11881 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
    11941 gtctgttgtt gttgttgact ctgtttatga cgctgtacgt gaacgttttg caacccacgg
    12001 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaacgg
    12061 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
    12121 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
    12181 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
    12241 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
    12301 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
    12361 aacggctcgt atcctgatta acaccccagc gtctcagggt ggtatcggtg acctgtataa
    12421 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tgggggggta actccatctc
    12481 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
    12541 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
    12601 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
    12661 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
    12721 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
    12781 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
    12841 cgccgcgaag attatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
    12901 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
    12961 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
    13021 tgtaactgac gacgctactg gtcagaaata tccgctggca gactatgcgc tgactccgga
    13081 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
    13141 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
    13201 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
    13261 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
    13321 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
    13381 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
    13441 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
    13501 ggctcgccgt cgttatgctg aaattgccga ccacttgggt ctgagcgcac cgggcgaccg
    13561 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
    13621 tattccgaaa tctatccgtg aagctggcgt tcaggaagca gacttcctgg cgaacgtgga
    13681 taaactgtct gaagatgcat tcgatgacca gtgcaccggc gctaacccgc gttacccgct
    13741 gatctccgag ctgaaacaga tcctgctgga tacctactac ggtcgtgatt atgtagaagg
    13801 tgaaactgca gcgaaaaaag aagccgctcc ggctaaagct gagaaaaaag cgaaaaaatc
    13861 cgcttaatca gtagcgctgt ctggcaatat aaacggcccc ttc
//
LOCUS       DAEEKQ010000103        13833 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.103, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000103 DAEEKQ010000000
VERSION     DAEEKQ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13833
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.103"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(30..788)
                     /gene="dsbG"
                     /locus_tag="KLK57_002832"
     CDS             complement(30..788)
                     /gene="dsbG"
                     /locus_tag="KLK57_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HBE0893149.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWILDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGGKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGVMTPTY"
     gene            1160..1723
                     /gene="ahpC"
                     /locus_tag="KLK57_002833"
     CDS             1160..1723
                     /gene="ahpC"
                     /locus_tag="KLK57_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HBE0893150.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            1894..3459
                     /gene="ahpF"
                     /locus_tag="KLK57_002834"
     CDS             1894..3459
                     /gene="ahpF"
                     /locus_tag="KLK57_002834"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HBE0893151.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(3580..4008)
                     /gene="uspG"
                     /locus_tag="KLK57_002835"
     CDS             complement(3580..4008)
                     /gene="uspG"
                     /locus_tag="KLK57_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HBE0893152.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVIR"
     gene            4229..5467
                     /locus_tag="KLK57_002836"
     CDS             4229..5467
                     /locus_tag="KLK57_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HBE0893153.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDILGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            complement(5698..6108)
                     /gene="rnk"
                     /locus_tag="KLK57_002837"
     CDS             complement(5698..6108)
                     /gene="rnk"
                     /locus_tag="KLK57_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HBE0893154.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(6338..7144)
                     /gene="rna"
                     /locus_tag="KLK57_002838"
     CDS             complement(6338..7144)
                     /gene="rna"
                     /locus_tag="KLK57_002838"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HBE0893155.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEAHASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKNFVIDKVGY"
     gene            complement(7258..8721)
                     /gene="citT"
                     /locus_tag="KLK57_002839"
     CDS             complement(7258..8721)
                     /gene="citT"
                     /locus_tag="KLK57_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HBE0893156.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLSVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            complement(8772..9650)
                     /gene="citG"
                     /locus_tag="KLK57_002840"
     CDS             complement(8772..9650)
                     /gene="citG"
                     /locus_tag="KLK57_002840"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HBE0893157.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEIAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            complement(9625..10176)
                     /gene="citX"
                     /locus_tag="KLK57_002841"
     CDS             complement(9625..10176)
                     /gene="citX"
                     /locus_tag="KLK57_002841"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HBE0893158.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            complement(10180..11712)
                     /gene="citF"
                     /locus_tag="KLK57_002842"
     CDS             complement(10180..11712)
                     /gene="citF"
                     /locus_tag="KLK57_002842"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HBE0893159.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANQEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            complement(11723..12631)
                     /gene="citE"
                     /locus_tag="KLK57_002843"
     CDS             complement(11723..12631)
                     /gene="citE"
                     /locus_tag="KLK57_002843"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HBE0893160.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            complement(12628..12924)
                     /gene="citD"
                     /locus_tag="KLK57_002844"
     CDS             complement(12628..12924)
                     /gene="citD"
                     /locus_tag="KLK57_002844"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HBE0893161.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            complement(12939..>13833)
                     /gene="citC"
                     /locus_tag="KLK57_002845"
     CDS             complement(12939..>13833)
                     /gene="citC"
                     /locus_tag="KLK57_002845"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HBE0893162.1"
                     /translation="GNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKT
                     EYEALFRQCGFSTLTSVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPF
                     TNGHRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYI
                     ISRATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQ
                     DMRYWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLH
                     YLQNLLEHSRQDAAARQKTPA"
BASE COUNT         3400 a         3503 c         3676 g         3254 t
ORIGIN      
        1 atcgggcatt atctgaacat aaaacactat caataagttg gagtcattac ccccataatg
       61 atattaagcg ttttctgatc gggcaacccc acggcctgtt gcagcgtatt ttccttactc
      121 atgtaataga tagccggcgt gacatttgcc cccagatcgt ccatcagttt ctcattgtca
      181 cttaacactt tcatttgctc tgtacttacg tttgcaggca cgtttagctt aagcttgcca
      241 ccagaggctt catattgttg ccaggttttt gcgggatctt tggaggcaag aattgccgct
      301 gctgtcgccg ggctttctgg cttgataacc ccaaccaaca atgttcttaa ttgcactttg
      361 ccagaatcta cccacgggcg cgcctgctgc cagaactgtt tacaatatgg gcagaacgga
      421 tcggcgaaga cgtagacaat gaccggcgca tcttttttac cgtcgaggat ccagtgggat
      481 tgttccatcc gttgccacat ttcgcgtccg gctggtgcgt aaatttcttt ttcgataagt
      541 gtgttactca ggttttcacc tttctcgttg tacatgtaac cagagatagc gtgcttacca
      601 tcgggagtca ggtagatggt gacgcccata tcctgatact ttccgagata acctttcatt
      661 cctccggggg catcgaacgt tttgatgatt gtaatgccct gtttttcaat cgcttttact
      721 ggggaaggaa gttcctctgc gaaggcgatt gcaggaagca gagccagtaa aagtatcttt
      781 tttaacatta atttgtcctt ttcagtcagt gcaaaagtct agtaaaaggc ataacctatc
      841 actgtcatag gtaagagctt agatcaggtg attgcccttt gtttatgagg gtgttgtaat
      901 ccatgtcgtt gttgcatttg taagggcaac acctcagcct gcaggcaggc gctgaagata
      961 ccaaagggta gttcagatta cacggtcacc tggaaagggg gccattttac tttttatcgc
     1021 cgctggcggt gcaaagttca caaagttgtc ttacgaaggt tgtaaggtaa aacttatcga
     1081 tttgataatg gaaacgcatt agccgaatcg gcaaaaattg gttaccttac atctcatcga
     1141 aaacacggag gaagtataga tgtccttgat taacaccaaa attaaacctt ttaaaaacca
     1201 ggcattcaaa aacggcgaat tcatcgaaat caccgaaaaa gataccgaag gccgctggag
     1261 cgtcttcttc ttctacccgg ctgactttac tttcgtatgc ccgaccgaac tgggtgacgt
     1321 tgctgaccac tacgaagaac tgcagaaact gggcgtagac gtatacgcag tatctaccga
     1381 tactcacttc acccacaaag catggcacag cagctctgaa accatcgcta aaatcaaata
     1441 tgcgatgatc ggcgacccga ctggcgccct gacccgtaac ttcgacaaca tgcgtgaaga
     1501 tgaaggtctg gctgaccgtg cgaccttcgt tgttgacccg cagggtatca tccaggcaat
     1561 cgaagttacc gctgaaggca ttggccgtga cgcgtctgat ctgctgcgta aaatcaaagc
     1621 agctcagtac gtagcttctc acccaggtga agtttgcccg gctaaatgga aagaaggtga
     1681 agcaactctg gctccgtctc tggacctggt tggtaaaatc taaatttcct tcgtctttcg
     1741 cgctaccagc tccggcgcga aatacttcgg aaattcacct aattcttcgg gtgctgcggc
     1801 acccgatttt cttccccgca ccatgatgca agctgcatcc aggtagccgt agaggccgct
     1861 tgcatgatga tgtttaaagc ccaggagata aacatgctcg acacaaatat gaaaactcaa
     1921 ctcaaggctt accttgagaa attgaccaag cctgttgagt taattgccac gctggatgac
     1981 agcgctaaat cggcagaaat caaggaactg ttggctgaaa tcgcagaact gtcagacaaa
     2041 gtcaccttta aagaagataa cagcttgccg gtgcgtaagc cgtctttcct gatcaccaac
     2101 ccaggttcca accaggggcc acgttttgca ggctccccgc tgggccacga gttcacctcg
     2161 ctggtactgg cgttgctgtg gaccggtggt catccgtcga aagaagcgca gtctctgctg
     2221 gagcagattc gccatattga cggtgatttt gaattcgaaa cctattactc gctctcttgc
     2281 cataactgcc cggacgtggt gcaggcgctg aacctgatga gcgtactgaa cccgcgcatc
     2341 aagcacactg caattgacgg cggcaccttc cagaacgaaa tcaccgatcg caacgtgatg
     2401 ggcgttccgg cagtgttcgt aaacgggaaa gagtttggtc agggccgcat gacgttgact
     2461 gaaatcgttg ccaaaattga tacgggcgcg gaaaaacgtg cggcagaaga gctgaacaag
     2521 cgtgatgctt atgacgtatt aatcgtcggt tccggcccgg cgggtgcagc ggcagcaatt
     2581 tactccgcac gtaaaggcat ccgtaccggt ctgatgggcg aacgttttgg tggtcagatc
     2641 ctcgataccg ttgatatcga aaactacatt tctgtaccga agactgaagg gcagaagctg
     2701 gcaggcgcac tgaaagttca cgttgatgaa tacgacgttg atgtgatcga cagccagagc
     2761 gccagcaaac tgatcccagc agcagttgaa ggtggtctgc atcagattga aacagcttct
     2821 ggcgcggtac tgaaagcacg cagcattatc gtggcgaccg gtgcaaaatg gcgcaacatg
     2881 aacgttccgg gcgaagatca gtatcgcacc aaaggcgtga cctactgccc gcactgcgac
     2941 ggcccgctgt ttaaaggtaa acgcgtagcg gttatcggcg gcggtaactc cggcgtggaa
     3001 gcggcaattg acctggcggg tatcgttgag cacgtaacgc tgctggaatt tgcgccagaa
     3061 atgaaagccg accaggttct gcaggacaaa ctgcgcagcc tgaaaaacgt cgacattatt
     3121 ctgaatgcgc aaaccacgga agtgaaaggc gacggcagca aagtcgttgg tctggaatat
     3181 cgagatcgtg tcagcggcga tattcacaac atcgaactgg ccggtatttt cgtgcagatt
     3241 ggtctgctgc cgaacaccaa ctggctcgaa ggcgcagtcg aacgtaaccg catgggtgag
     3301 attatcattg atgcgaaatg cgaaaccaac gtcaaaggcg tattcgcagc gggtgactgt
     3361 acgacggttc cgtacaagca gatcatcatc gccactggcg aaggtgccaa agcctctttg
     3421 agtgcttttg actacctgat tcgcaccaaa actgcataag aagaagtaag attcacctgc
     3481 aattgcttag ccgccggggt caaacctggc ggctttttta tggcattaaa aagccctgcc
     3541 gggatgttcc aggcagggcg gcataaatac ttttatctgt tagcgaatga ccagtacagg
     3601 cagattggcg tggcggatca cgctcgaggc gttagaacct aacagatggg tcgaaatcga
     3661 tgggttgcga gaaccgataa ctacaacatc agcccccagt tcttctgcca actcattgac
     3721 ttcatcccgc acgctaccaa aacggacatg ttgtttaatg cgggaaggat cgatggtgaa
     3781 gtggctgacc atcgtttgca gacgttcttc tgcttcatgt tgcagatgct cttcaaaacg
     3841 acgcacatca gcggcaaaac ggtgcaggct caggctggct gacccgggta gtacgtgaag
     3901 tagatgaata actccgtcat cctgggcgag gaattcagcg tggcgaacag ctttgtcgct
     3961 caattccatt tcaaatacat caactggcat aatgattgtc ttatacataa ccctttctcc
     4021 ctgttaatca tgaacaaatc attcgccatg attataatat ttatccctga tatttgtctg
     4081 gttctttttc cttacgaacg gtttctgtga ttaatatatt ctcactgaac accaggaatt
     4141 ctcccaaaac ctgcggtacc gcccgttttc ccgctgtgat agctaccctt aaagactgac
     4201 tcttttttga actgtctctg gaggttgcat gaaagcattg acttatcacg gcccacatca
     4261 cgttcaggta gaaaatgttc ccgatccggg cattgaacag gctgatgata ttattctgcg
     4321 tattacggca acggcgatct gtggctctga cctccatctt tatcgaggca aaatacccca
     4381 ggttaaacat ggcgatattt tgggtcatga atttatgggg gaagtcgttg aaaccggaaa
     4441 ggacgtaaaa aatttgcaaa aaggcgaccg ggtggtaatt ccgtttgtca ttgcttgtgg
     4501 cgactgtttt ttctgtcgaa tgcagcaata tgccgcctgc gaaaatacca atgcgggtaa
     4561 aggcgctgcg ctcaataaaa aacagatacc agctcccgcg gcattgtttg gttatagtca
     4621 cctgtatggt ggcgttcctg gtggacaggc ggaatatgtc cgcgtcccta aagggaatgt
     4681 ggggccgttt aaagtaccgc ctttgctttc agatgataaa gcccttttcc tttctgatat
     4741 tctgccaacg gcatggcagg cagcaaaaaa tgcgcagatc caacaaggtt caagcgttgc
     4801 agtctatggt gccggtccag tgggattgtt gacaatcgcc tgtgcacggt tgctcggtgc
     4861 ggaacagatt tttgttgttg atcatcatcc ctaccgcttg cgtttcgccg ctgaccgcta
     4921 cggcgcgatc ccgattaatt tcgatgaaga tagcgatccg gcacagtcaa ttattgaaca
     4981 aacggcaggt caccggggcg tggatgcagt aatagacgcc gtcggttttg aagcgaaagg
     5041 cagcaccacg gaaacggtgc tgactaacct gaaactggag ggcagcagcg gtaaagcgtt
     5101 gcgccagtgt attgcggcgg tcaggcgtgg cggcattgtt agcgtaccgg gcgtctacgc
     5161 tggatttatt cacggtttcc tgtttggcga cgcctttgat aaagggttga cgtttaaaat
     5221 gggacagacc cacgttcacg catggctggg tgaattatta ccgttaattg agaaaggatt
     5281 actgaaacca gaagaaattg ttacccacta tatgccgttt gaagaggccg cccggggata
     5341 tgagattttc gaaaaacgtg aagaggagtg ccgtaaggtg attctggtac ccggtgcaca
     5401 aagcgcagag gcggcgcaga aggcggtttc aggtctggtg aatgcgatgc cggggggaac
     5461 aatatgatcg tcaggagtgg ttttcgaggt aaaggacagc catgacgata atcgccgcca
     5521 taatcagaaa tcctatcagg atgtaaaatg cttctgccat ggttattccc acaaacgaaa
     5581 cggaataatt ttgcagcaaa agtgaacagt gagaaccagg aaaaattgct gattttgcgt
     5641 aaagaggatg cgagtgcatc ctctgggcaa agcgagttat cgcttgtgca gatgggatta
     5701 aagcaggtag tcgccagcag cttctggctg gtattcgagt tccagcactt caaggtgggt
     5761 tgcaacgccg cccggaagtt cccagtgaat ggaatcgcca acgcgcagcc ccagcagtgc
     5821 ggcacctacc ggagccataa cggaaagctg agtattgcta tcggtcattt ttgccggata
     5881 caccagcgtg cgcacacgca cttcgccatc gctaagattg cggaatttaa cccggctgtt
     5941 cattgtcacc acgtcgtgtg gcatctcttc tggcgaacac atttgggcgc gatccaactc
     6001 tgcgtttaac gcgtcggcga ttggcaaacc agcataggcg ggttgctcca gcagaatatc
     6061 gatgcgttcg gcatccaggt cgttaatgat gatagttggt ctggacattt ttactccatg
     6121 tcgtcggtac tgcgagtgtc gcagataaac atacccaaaa gaaaaccctc accgtcaggc
     6181 ggcgagggtt taactcacat gatgatactg actgttgctc actctttgaa gtgatttgcg
     6241 tcacattcag ggaattcctc aatgcagcgc attatgtata attcttaatc gtcacggttt
     6301 atggaagacg aattagcgtg ttttgtaaat cagatgatta ataacccact ttatcaatca
     6361 caaagttttt gccacagttg cctgggtgtg gttgtggcaa gaatgagttt gcagaaagcg
     6421 gagcgttgat ggcgtcagct ttgatcgaga tctgaatttc agtcaaatac gcagggttac
     6481 cctggcacgt tagcttaact gctttcacgt tctcttttcc ccagcttttg gcaaaggcgg
     6541 cgtcaaagtc acggcggctc actgttttac cgtaattatc cgcaaggaat tttccggctt
     6601 cgctctcttt aatttcttga ttcaggcgta ccatcgtacc gaagtatgcg tccggatcaa
     6661 aaccaaagca ggcaccgtgt ttggcatatt cgtagcgttc caggcaggaa cgtccgccag
     6721 ctcctggcat gacttcactt agtttagcgg ccgtttccag tgataacccg gtttccggcg
     6781 atgaacacat ccggctggcg tgcgcttctg gtagattcgg gattgggcga gtagcgcaac
     6841 cgaagcgcat ccagcggcgt tcatcaacac cacgggcagc aaccgattta ggcaatcctg
     6901 gccacagacc atgtacggtc agaaaatcag ctttgttggt cgtttcggtt tgtaggcgac
     6961 attcatctcg ttcgttacga tttcgatcgt gttgactctg gcaaaatccg gtttgccagg
     7021 agagggccag gacatagcga tcaaaatcgc catactgttt tgcctgcaac gctacggcgt
     7081 tggcagaaga gaagggaagc agagaaaccg cgagcaacgc ggcgttacgc cagaatgctt
     7141 tcataatggg tgtggaactc atacatacac tgaatactat ctattaaatc ataaaaaagc
     7201 ccgccaggtg ggcttacctg gcgggcgtga tgatttattc agcgtttggc gaacgtatta
     7261 gttccacatg gcgagaatcg gccagccaac caacagcaac atagagatgt aaatcacccc
     7321 gaagattgcg ccaagacgcc agtaatcttt tgatttcaca tagccacagc cgtaaataat
     7381 caccccagga ccggttgcat acggcgtcag acagcccatg ataccgatag acagcaccag
     7441 caggatacac agttgttcca ttggtacgcc cggaatacct ttaccgacgg ccagaataac
     7501 cgacagcatg gttgcggtgt gcgcagacag gctggcaaac aggtagtgtg caaagtagaa
     7561 caccagaacc agtacaatca ccgttgcgtt tggtgagaat ccttccaggt gcgtactcat
     7621 ggtaccggcg aaccagtcaa taaagccaga acgagtcagg ccgttagcca tcacaaccag
     7681 agttgccagg ttgaccagcg tgttccatgc gctgttatag cgggtaatat ctttccaagg
     7741 cacaacgtgc agggccagca ttagcgaaac tgccagcaga ccaactgcag tagcattaat
     7801 gacttcactg ccaaaaaccc acaaacctaa gctgagcaat acaaggccaa tcagtgtcca
     7861 ctctctgcgt gtcagcgcac ccatggtttt cagttcatca cccgcccagg ttgccacttc
     7921 ttcactgtgt gtgatttccg gtttgtacag cacgtaggaa agccacggcg caatgataag
     7981 caagataacc ccaaccggca ggaagcagag gaaccactgc aaccagctaa tctggatacc
     8041 ggcaattttg ctgacgaact ccagacccag cacgtttggt gccgcaccgg tgacaaacat
     8101 ggacgaactc agactggtac taatgaccat catccacatc aaatagccgc caatacgacg
     8161 cgcggacgga tcgttgggga atgatttaaa cagcggcggc aggtttttaa tgaccggaaa
     8221 aaccgtaccc ccggtacgcg cggtgttgga cggtgtaaac ggtgccagca gaatgtcgat
     8281 aatgacaatc gcataaccca acgtcagcgt gcgtttgccc atgaatttca ccaggaaaag
     8341 ggcaatgcga cgacctaacc cggaaacttc ataccctaat gcaaaaataa atgcgccaaa
     8401 taccagccat accgtggtgc tggaaaaacc agccagaccc catttcagcg cctgtttttg
     8461 cgcattaaac gctgggtcag ctaattcttt ggcatcaaag agcaggtaat tactgccaat
     8521 aacgcaaata gtaaccgcaa taaaactgat cgctgttgcc ggaattggct cgaggatcat
     8581 gccgacaatc attgccacaa acacagcgaa gtaatgccat gcctgtggtg gcataccgtc
     8641 ggggacaggg ataagaaaca tgacacccat caccaccagt ggggccaata gtttccatat
     8701 attatctttt gctaaagaca tacgggttct ccgaaaatta atatttccaa atttatcaag
     8761 tgcttaaata attaaatctg tgctaaaaac caggtaagga tcagcaggtc ggcactgccg
     8821 cctggactga gatttcgttc gatacactcc ctgtcgaact gccggagata atcgagatcg
     8881 gcgggggttc gaatgccccc tttttgcaat aatgtttgcg cctcgcgctg tagccagcgc
     8941 aggcccccct cgccaccgcg cgatgcaacg ttggtatcgc cgttgatcgc catcagtagg
     9001 agcaaggtat cgagcaatgc cagttcagga tctaacccct gatccagcag agtgaggtaa
     9061 tgcggcaagg cgtgattgat caccagtgga taacccgctt cggcttcacc gcgtgcgccg
     9121 gtaaggccaa gctgctggta caaccgttga cctgccgtca gttgtgaatt attggtacgc
     9181 agttctcgat cggtcaggcc acggcagaaa cttgccgccg tagaacaaac ggttgttggc
     9241 gttaccggtt ggttgagttg aagcaaacgg ccaattgccg cacatagtag ccctaaagaa
     9301 aaaatgctgc ctttatgcgt gtttacgccc gcagtggcgc ggaacatatc accttcgcaa
     9361 gccataccaa ttgggcgtaa tccgtggagt accgcttctg gtgctatttc cgcactacag
     9421 gcaccaaatt caatgaaacg gggtagccag ccctgaatcg ccagcgcgct gcggtggaaa
     9481 tcttccagcg ccatatcttt atgtgcaccg cagttaatcc gatccacgag gcctggtttc
     9541 ggtgacagat tgacttcagt cagcatggcg cgccagccca gcagggcgta ctcatcgatc
     9601 aatgacgtcg caagctttgt ggttttagtt gacgttgcag gcatcgacat cgttcagcag
     9661 tgcctccatg cggttgagta aatcggtcag ttgatgggtt tttccacgcg cgcagacggc
     9721 tgcgctttgc tcgcacaaca ggcagcggcg aggcggcagt gaatagtcgc ggcgggagag
     9781 aatttcgcct tcgggcgtca ggacatcgat atcccataac cgcccgagag gatgactatg
     9841 ttcaagctca atggtggcga gcttgaggtc gcgagccggg gcggcaatgc tcaacatgcc
     9901 ctccggcccg ctggcggaaa ccagtgcagc ctgctcctga atttgccagc cctgttttgc
     9961 ggctaaggca cgcaaggctg tcacgccatg attaaaaatt cggcgtgtga cctcgctgtc
    10021 tttaatcggc ccaggcgcaa ccacggtaaa ggagaccagt ggaacaggat ggcgcttgag
    10081 ccagacgtgt tgccgtgctt gcctttcatc ccggctgacg agcagctcgg gaattgacac
    10141 cgcatggtgg ctggcgagtt caggaagcag gtgcatggct tattccttca cctgatgcac
    10201 aacatcgatc accgagccat cgcggtaacg cacaacggca acgacgcggt cggtgaattc
    10261 aatcggctgt ggttcaccgg tcagcagacg cgcgcgttcg cgcagccact caatggaaac
    10321 cactttaatg cccgcttcct gcagacgttc tgccagttcc ggacgtgccg ggttaactgc
    10381 gataccgtgg tctgtgacca gaatatcgac actggagcct ggggtgatgc aggtcagcac
    10441 gttatccacc agagtcggaa tacgaccgcg taccagcggc gcgacgatga tggaaagcgc
    10501 agaggcaatc gcggtatcgc agtgaccacc ggaagcacca cgcagtacgc cgtcagagcc
    10561 ggtcagcacg ttaacgttga actgggtgtc aatttccagc gcgctcagta ccaccacgtc
    10621 gagacgatca accgatgcgc ctttcgaacc ccagttagcg tactggttgg cgctgatttc
    10681 gatgtgattg gggttacggg ccagcgattg cgcagcatgg ctgtcaaagc tctgcacatc
    10741 cagcagtttg cggatcagac ctttttcgtg cagatcaacc atcgtcgcgg taataccgcc
    10801 gagggcgaag tcggcgcgaa tatcgcggct acgcattttg tcttccagga aacgggttac
    10861 cgccagcgat gcgccgccgg tgccggtttg catggagaaa ccttctttga agtagccaga
    10921 gttgacaatc acatccgcag cgctacgggc aataagcagt tcgcgcgggt tagtggtcat
    10981 acgggtcgcg ccagcgccga tttttgcagc atcgccaacg cggtcaactt tgacgatcaa
    11041 atcaacctga tcttgctcaa tgcttgccgg attatgcgga taaggcagca gttcttcggt
    11101 aagcatcaca acctgttttg cgttgtcggc atcaaccatt gcatagccga gggagccgca
    11161 gcaggcttta ccggtgtaac cgttggcatt accgaattca tcacaggacg ggacgccgag
    11221 gaaagccacg tcgatattca gttcgccgct ctgtaccaga tgcacacgtc cgccgtgaga
    11281 gtggatttgc accggttccg caagcagacc acgggaaatt tcttctgcca gtggaccgcg
    11341 caggccggag gtataaatgc gggtaaccac gccctggcga atgtgttcta ccagtggcgc
    11401 atggcaatca ctcagggagc tggacgccag ggtcaggttt ttaaagccca tcttagcgat
    11461 gacgtccatc accatattga cggtcaggtc accgccacgg aaagcgtgat ggaaggaaac
    11521 cgtcatgccg tcctgtaaac cagagcgacg aatcgcttct tcctggttgg cgcacagttt
    11581 gcgatcgcgc gctttttcag cctggtaggt ttgctttggt gagttctgga aagcggcaag
    11641 atcgcattca gcgcgacgat tccaggccgc tacccgttct tgtcgttgag attgttcaat
    11701 tttctgcgtc attttgattg ccttattctt cgcggatgcc ggaaagttct gcacgggaga
    11761 gcaccagacg ggcacgatcg ataaccggac cgtccaccat cttgccgttc agggaaacca
    11821 cgccgaggcc ttcgcgagcg gcggcttcag cggcttctac gacgcggcgg gcgtgatcca
    11881 cttctttctg ggtcggtgcg tagaggttgt gcaacagatc aatctgacgc gggttgatca
    11941 gcgatttgcc gtcaaagccc agctgtttga tgtgggcggc ttcttgcagg aatccggctt
    12001 cgttgttagc gtcggaatag acggtatcga acgcctgaat acccgcagag cgcgcggcct
    12061 gcaaaatgga acagcgtgcg aacagcagtt cagttccttc cggggagcgt tctgtacgca
    12121 ggttgcgcac atagtcttct gcaccgaggg cgataccgat caaacgctcg gaagcgtgag
    12181 cgatttccac tgcgcgggta atccccagcg gagattcaat cgccgccagc aggccggtgc
    12241 tgccgggttc acgaccacag gctttttcga tacgcaggat ctctttttcg atatccagaa
    12301 catcctgagc ggtatcggtt ttcggcagac gcacaacgtc cgcaccaccg cgaacgacgg
    12361 cttccaggtc gttgacaccc cattcggaat ccagcgcgtt gacacgcaca atggtttcaa
    12421 tatcgcgata cagcggatgt tgcagcgcgt ggtaaaccat gcggcgggcg gtgtcttttt
    12481 cacgcaatgc tacggagtct tcgaggtcaa acatcagggc atcagccggg tagatgaagg
    12541 agttgctgac catcgcggca ttggcaccag gcacaaacaa catgctgcgg cgggtgcgag
    12601 ttttacgttg ttgcagcgaa gcggaaatca ttggcaatcc tcccatggca gagccgggat
    12661 accgctggcg cgtgccagca gggcttccag tcgtgcacgt aaaatgcagt ccagtgcgcc
    12721 tttgtcatcg acattcagct gtacgccgcg cacgttgtag cgggcgagaa cgtccagaat
    12781 ggtggtgcga attgcatcgc caaactgttt ctcaacgctg ctattgattt gcaggtcgat
    12841 atcctgcgta tcgagtgggg cgatgcgtat catcacatcc ccagactcaa gggtgcctgc
    12901 aacggcgggc tggtttattt tcatttttca cctgtttctc atgcgggggt cttttgacga
    12961 gctgccgcgt cctggcggga gtgctcaagc aggttctgca aataatgcag cgtgactgca
    13021 gggaccagcg gcgcgatagc cgtgagatcg tttttcgcca gcagttgacg tacccgggaa
    13081 gcggatatcg gcatctcctg gtaacgcagc cgctcaattt caaccagttc gatgggcggt
    13141 gcggagatag tcggcgtttc cagccagtag cgcatatcct ggttgtactg ggcggtaacg
    13201 cgacaaaagg gttcagtacc gacaaagcgg tgagttacac ccagcgcggg agcgaggtac
    13261 tgacggaaaa tcttcagatc aatttcggtg taacaatggt taatgacgct ctgttcttta
    13321 atgaagtagc aagggaacgt agcgcgggag atgatgtatt cggagccgcg atgcacagtc
    13381 aggcgtggaa tatcggcggt gccttttaac accagatcca accggtcttc ataggggaag
    13441 cgtgaagaat cttctttgac taaaaacaga tgcaaccagt cgcactgtgc cgcagcttgt
    13501 tgaatcagat aacggtgacc attcgtgaag ggattggcgt tcatcacaat gcagccaatc
    13561 ttgttccctg gatgacgaaa ttttttcagc gattcggcat agcgtttcag ccgcgtggcg
    13621 ctgttttcca tcagcaccat cacgccgggt acgctggtca gcgtggaaaa accgcactgg
    13681 cggaacagcg cctcgtattc ggttttggta taaataaaca gatgcgtgct gtgccgctca
    13741 taggcgaggt ttatcaattc agtggctaat gtcagcgcca gtccttcacc gcgaacggat
    13801 tcactgatag caacgcattt aataatattt ccg
//
LOCUS       DAEEKQ010000104        13794 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.104, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000104 DAEEKQ010000000
VERSION     DAEEKQ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.104"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..147)
                     /locus_tag="KLK57_002846"
     CDS             complement(<1..147)
                     /locus_tag="KLK57_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HBE0893163.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKA"
     gene            complement(344..475)
                     /locus_tag="KLK57_002847"
                     /pseudo
     CDS             complement(344..475)
                     /locus_tag="KLK57_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310047.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
     gene            476..1021
                     /locus_tag="KLK57_002848"
                     /pseudo
     CDS             476..1021
                     /locus_tag="KLK57_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
     gene            complement(1063..2064)
                     /gene="gap"
                     /locus_tag="KLK57_002849"
     CDS             complement(1063..2064)
                     /gene="gap"
                     /locus_tag="KLK57_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBE0893164.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            2253..2783
                     /gene="cybB"
                     /locus_tag="KLK57_002850"
     CDS             2253..2783
                     /gene="cybB"
                     /locus_tag="KLK57_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HBE0893165.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVMYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3028..3201
                     /locus_tag="KLK57_002851"
     CDS             3028..3201
                     /locus_tag="KLK57_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893166.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="KLK57_002852"
     CDS             complement(3273..3422)
                     /gene="hokB"
                     /locus_tag="KLK57_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HBE0893167.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            3821..5461
                     /gene="trg"
                     /locus_tag="KLK57_002853"
     CDS             3821..5461
                     /gene="trg"
                     /locus_tag="KLK57_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HBE0893168.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASCIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMKDEPAGRNEIGRLNRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            complement(5499..6422)
                     /locus_tag="KLK57_002854"
     CDS             complement(5499..6422)
                     /locus_tag="KLK57_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0893169.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            6639..7982
                     /locus_tag="KLK57_002855"
     CDS             6639..7982
                     /locus_tag="KLK57_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE0893170.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8207..9862
                     /gene="opgD"
                     /locus_tag="KLK57_002856"
                     /gene_synonym="mdoD"
     CDS             8207..9862
                     /gene="opgD"
                     /locus_tag="KLK57_002856"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HBE0893171.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYALKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            10002..10226
                     /locus_tag="KLK57_002857"
     CDS             10002..10226
                     /locus_tag="KLK57_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HBE0893172.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            10289..10825
                     /gene="rimL"
                     /locus_tag="KLK57_002858"
     CDS             10289..10825
                     /gene="rimL"
                     /locus_tag="KLK57_002858"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HBE0893173.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(10820..11800)
                     /locus_tag="KLK57_002859"
     CDS             complement(10820..11800)
                     /locus_tag="KLK57_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HBE0893174.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIRDSLVYGQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ISGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            11924..12916
                     /gene="tehA"
                     /locus_tag="KLK57_002860"
     CDS             11924..12916
                     /gene="tehA"
                     /locus_tag="KLK57_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HBE0893175.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSENGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            12913..13506
                     /gene="tehB"
                     /locus_tag="KLK57_002861"
     CDS             12913..13506
                     /gene="tehB"
                     /locus_tag="KLK57_002861"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HBE0893176.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDRQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
BASE COUNT         3540 a         3197 c         3482 g         3575 t
ORIGIN      
        1 tgccttacgg gcatcctcgg cctgaccatc gggtatacgg gaaatgacag cctctgtagc
       61 agggtttacc acatcaatcc atgcgtctcc acgccaggta acaaactgtc catcgatata
      121 cataggatgt tgaacgggta ctgacatggg cgactcctgt gatttatatg tttgttttcg
      181 gtgaatatat tgttaattaa catcaatcat cagagagacg gcatcgggaa aggacaattc
      241 tgtgaagggc ttcataaaaa caagtgtaaa aggaataaaa ggcagaaata aaagtcagga
      301 gtcatgcccg tttgcaaaca agtggttcac aaacgggcaa tttttaacgt aacgagcgac
      361 tttccatcgc ctcgatgagc accgcatcat gtttcagcgt ttgccatgca tggatgactt
      421 ctgccggaaa atcaacgtga acgataaaat ctcgcccgcg gggaccgtag ccattgatca
      481 gttcgtcaat cggctgggtg aagcgatgca ggcggttaaa tttggtaacc ccgctgaacg
      541 caacgacatt gcgatggggc cgttgattaa cgccgcggcg ctggaaaggg ttgagcaaaa
      601 agtggcgcgc gcagtagaag aaggggcgag agtggcgttg ggtggcaaag cggtagaggg
      661 gaaaggatat tattatccgc cgacattgct gctggatgtt cgccaggata tgtcgattat
      721 gcatgaggaa acctttggcc cggttctgcc ggtggtcgca tttgacacgc tggaagaggc
      781 tatctcaatg gctaatgaca gtgattacgg cctgacctca tcaatctata cccaaaatct
      841 gaacgtcgcg atgaaagcca ttaaagggct gaagtttggt gaaacttaca tcaaccgtga
      901 aaacttcgaa gctatgcaag gcttccacgc cggatggcgt aaatccggta ttggcggcgc
      961 agatggtaaa catggcctgc atgaatatct gcagacccag gtggtttatt tacagtcgta
     1021 atgagtaaaa gaggcggagg ttttttcctc cgcctgtgcg cgtcagagtt tagcgaattt
     1081 ttcgagggtg cgaataagct gcgtgacgaa gccatattcg ttatcgtacc aggcgaccgt
     1141 tttcaccagt tgtaaatcgc ccacggcggt aatttccgtt tgcgtggcat caaacaccga
     1201 accgaaatgg ctgccaatga tatcggaaga gactatttct tcatcggtat aaccaaatga
     1261 ctcgttattg gtggttgctt gtttaagtgc gttattcacc tcttcggcag tcactttttt
     1321 tccgagaatc gataccagtt cagtgaccga acctgttttc accggcacgc gttgcgcatg
     1381 acctttcagt ttgccgctca gttccgggat caccagacca atggcttttg ccgcccccgt
     1441 agtgtgggga atgatatttt ctgccgctgc gcgtgaagca cgtaaatctt taccacgtgg
     1501 gccatccacc agtgactggg tgccagtata ggcatgaatg gtcgtcatcg tgccgacttc
     1561 tatcccgaaa ctgtcatgca aggctttggc catcggcgca agacagttag tggtgcatga
     1621 cgccacggaa acaatggtgt cgttgccatc cagagtgtcg tcattgacgt tataaacgat
     1681 agttttcatt tcaccggcag gggcggaaat caacaccttc ttcgcaccag catcaagatg
     1741 tgcctgcgat ttctcggcgg aggtataaaa gccagtacat tcgacaatga tttctgcacc
     1801 tttcgctttc cacggaatat ttttagcctc tttttcggcg taaaccgcga tacttttccc
     1861 atcaacgata agtgaatctt ccgtaaaatc aacgctccag gggaatggtc cgtagtttga
     1921 atcatgtttc agcaggtagg cgagaatttt tggggaagtg agatcattaa tagcgacaac
     1981 gtctatgttg cttttgactt caagtaatcg acgcaacacc agtcgaccga tacgaccaaa
     2041 accgttaata ccaactttac tcatggtttt ctcctgtcag gaacattcgg atgaaaattg
     2101 atcctttcca ggcttagacc aggattgtgg gatgggcaat ctccattctt acagtgaaac
     2161 gtaacgtact gaaaacgggt gaacaatatt taatgaaatt ttgagaaaag cccgttatgt
     2221 taacggaaaa ttatgttaaa gcaggaaatg ttatggaaaa taaatactca aggttacaaa
     2281 tcagcattca ctggctggtc tttttgctgg ttatcgcagc gtattgcgca atggagtttc
     2341 gtggtttctt cccacgtagc gatcggccac tcatcaacat gattcatgtt tcctgcggca
     2401 tctcaattct cgtgctgatg gtcgttcgtc ttttgttaag gctgaaatac ccaaccccgc
     2461 cgattatacc taagccaaaa ccgatgatga cgggactggc gcatttggga catttggtga
     2521 tgtatctcct gtttattgcg ctgccagtga ttggtttggt gatgatgtat aaccggggca
     2581 acccgtggtt tgcgtttggt ttgacgatgc cttacgcttc agaggccaat ttcgaacggg
     2641 ttgatagctt aaagtcgtgg catgagacgc tggcgaatct gggatatttt gtcatcgggt
     2701 tgcacgctgc ggctgcactg gcacaccact atttctggaa ggacaacaca cttctacgca
     2761 tgatgccgcg taaacgttcc tgaaggatat ttcaagaaaa tgcctgtact aaaaccgacc
     2821 cgtggtacag gcgaagaata cgggtctaca tcggaagcgc ctattatatt tatttgtatg
     2881 acaaataaaa ccataatcct tgcccatacg tccatctggc ttatttttaa tcaattcacc
     2941 cgatctttga tctcatcaac ggtatcaaaa taaaccagcg tataacgttc aaatcgaaca
     3001 taagattcga taaaccatgg aggttatatg aaaaaactgg cacttatttt gtttatggga
     3061 acgcttgttt ccttttatgc cgatgccggg cgcaaaccct gttctggttc gaaagggggg
     3121 atctcacact gtacggcagg cggcaaattt gtctgtaatg atggttctat tagtgcatcg
     3181 aaaaaaacat gcactaactg aagtgtaaaa ggggtgccat gagaaaatga atctgctagt
     3241 cagatgcgcg gggaaaatcc ccgcgcttgc ccttacctgg acgtgcaggc catgagcgca
     3301 gcaacctcct tatcaccgtc ccggaaccgc agttcgtaga gcgtttgtcg ggtcaggagt
     3361 gtgaatgtca gaatcgtaat gcagataatg agcagacaca ctaccagagg gttgtgcttc
     3421 atagcctttc tccttgccgg atggcgggta agaggctaag atctgaattg ctaggttcat
     3481 tcgttggcct cggttgatag aaatatcggt cggggccttc gtctttctga ttcccggtta
     3541 gcctgaaaac agaaagtctc aggcacccgc aggcatccta tgaggtttcc ttagggacga
     3601 aaataatcac ttcacgaaat tgcgtgctgt tttccagaat ttttcgtcat tcgggttagc
     3661 cagtttagcc attcgttact ctcttcattc caatagcatt aattttctct gcaataattg
     3721 ttgtaaaaat gtgacgcaaa gaggtttttg gtcataagta attaccgtca agtgccgatg
     3781 actttctatc aggagtaaac ctggacgaga gacaacggta atgaatacaa ctccctcaca
     3841 gcgattaggt tttttgcatc acatcaggct ggttccgtta tttgcctgca ttcttggcgg
     3901 tatcttagtt ctattcgcat taagttctgc tctggctggc tatttcctct ggcaggccga
     3961 tcgcgatcag cgtgatgtta ctgcggagat tgagattcgg accgggttag cgaacagttc
     4021 agattttttg cgttcagccc ggatcaatat gattcaggcc ggggctgcga gttgtattgc
     4081 ggaaatggaa gcaatgaagc gaaatattgc gcaagccgaa tcggagatta aacagtcgca
     4141 gcaagggtat cgtgcttatc agaatcggcc ggtgaaaaca cctgctgatg aagccctcga
     4201 cactgaatta aatcaacgct ttcaggctta tatcacgggt atgcaaccta tgttgaaata
     4261 tgccaaaaat ggcatgtttg aagcgattat caatcatgaa agtgagcaga tccgaccgct
     4321 ggataatgct tataccgata ttttgaacaa agccgttaag atacgtagca ccagagccaa
     4381 ccaactggcg gaactggccc atcagcgcac ccgcttgggt gggatgttca tgattggcgc
     4441 gtttgtgctt gccctggtca tgacgctgat aacatttatg gtgctacgtc ggatcgtcat
     4501 tcgtccactg caacatgccg cacaacggat tgaaaaaatc gctagtggcg atctgacgat
     4561 gaaggatgaa ccggcgggtc gtaatgaaat cggtcgctta aatcgtcatt tacagcaaat
     4621 gcagcattca ctggggatga cagtagggac tgttcgacag ggtgcggaag agatttatcg
     4681 tggcaccagc gaaatttcag ctggcaatgc ggacctgtca tctcgcaccg aagaacaagc
     4741 ggcggctatc gaacaaactg ccgccagcat ggagcaactc actgcgacgg tgaaacagaa
     4801 tgcggataac gcgcatcatg ccagcaaact ggcgcaagag gcttctatta aagccagcga
     4861 tggcgggcag acggtttccg gtgtagtaaa aacgatgggc gctatctcca cgagttcgaa
     4921 gaaaatttct gagatcaccg ccgtcatcaa cagtattgct ttccagacga atattctggc
     4981 actgaatgct gccgttgaag ccgcgcgagc gggtgagcaa gggcgtggat ttgccgttgt
     5041 cgccagcgaa gtacggacac tcgcaagtcg cagcgctcag gcggcgaaag agattgaagg
     5101 cttgatcagt gaatcagtca ggttaattga cctggggtcg gatgaggtgg caacggccgg
     5161 gaaaaccatg agcactattg ttgatgccgt cgcgagtgtc acacatatca tgcaggaaat
     5221 cgccgccgcc tcggatgaac aaagtagagg cataacgcag gttagccagg cgatttctga
     5281 aatggataag gtgacgcaac agaatgcttc tctggtagaa gaggcctcag cggcggcggt
     5341 gtcccttgaa gaacaggcgg cacgattaac tgaggcggtg gatgtattcc gtctgcacaa
     5401 acattctgtg tcggcagaac ctcgcggagc gggtgaacca gttagtttcg ctacggtgtg
     5461 aaaatgttca aggagggatc gacagatccc ttcacctttc agaacggcat tgattttcga
     5521 atagcgttaa tcatcaactg gcaaccagaa gagaacgtcg catctacgcg ggtcagtatt
     5581 ccaatcggtt cgcctgcacc atgtcccgga acaggcaggg ccaccagcgt ggcatgacgc
     5641 aggtcgtctt ttacagcgcc agaagggaca aaccacacgt aatcgtattc aaccgtaagt
     5701 tgacgagata gcgaagcaga cagcgtttcg atacaacccg aaggaatttt acagccctgg
     5761 ctctgcacta atgcatctga atgctggcgt ggcgcagtgc cttctggtga tacaacgacc
     5821 ggccattcca gcacccggct tagcgttacg ttctcctgaa gtagcgggtg attagggcgg
     5881 acaaccagct tcagcgattc aagaaacagc agttcgtaat taagcccggt catcagttca
     5941 ggatctgaca tccgaccaat gccgatatcg atttccccgg tttttaaacc cgccagaatc
     6001 atagggttac tcattgtcgc aacttgcaag gtcgtctctt tttgttgctg atgaaactga
     6061 cctataaccg aaggtaatat ccccagtgcc gcagtaggta gtgcaccaac cctgacgaca
     6121 tcattattaa gaccttcttt acgatgaagc gactgtccgg cagtgttgat ggcgtcaaga
     6181 actctgactg catgcgttaa aaattgttcg ccgggtaagg taagttgcgc cccctgacga
     6241 ccacgctcaa acaagcgagc gcccgtcagc tgctccagtt cattcaatgt cttagagagc
     6301 gcaggttgac tcaaattaag ggtttcagcc gcgcgcccca aagttccttg ttgtgcgaca
     6361 gctacgaatg tatgaaggtg gcgcaaacgg atgcgctgac taaacagact atttttttcc
     6421 ataagcgatg ttaaaaacga agcggtgtcg ctgacaagtg aagttgtttg attatgataa
     6481 cttgattgca aaatattatt aacaattaaa gcaattatgt tacagcaaaa tggataatat
     6541 tgatgttttc gcggcgagat cacagtttgt aaattcttcc cgccagagtg aatgcggtta
     6601 cctacactcc agattactga ccactggagg cagacactat ggcgaacagc atcacggcgg
     6661 atgagattcg ggaacagttt tcgcaggcaa tgtcagccat gtaccagcaa gaagttccgc
     6721 aatatggcac gctgctggaa ctggtagctg atgtgaatct ggctgtgctg gaaaacaatc
     6781 ctcaactgca cgaaaaaatg gtaaatgcag acgagctggc gcgactgaat gttgaacgtc
     6841 atggggcgat tcgcgttggg actgcacaag agcttgctac tcttcggcgg atgtttgcca
     6901 ttatggggat gtacccggtg agctattacg atctctcgca ggcaggggtg ccggtacatt
     6961 cgacagcatt tcggcccatt gatgatgctt ctctggcgcg taatcccttc cgcgttttta
     7021 cctccttact ccgccttgag cttatcgaga acgaaatttt gcgccagaaa gcggcggaga
     7081 ttctgcgtca gcgcgatatc ttcaccccac gttgtcgaca actgttagag gaatatgagc
     7141 agcagggcgg ttttaacgaa acacaggcac aggagtttgt gcaggaagcc ctggaaacgt
     7201 ttcgctggca ccagtcagca acggtagatg aagaaaccta tcgcgccttg cacaacgaac
     7261 atcggttgat tgctgatgtg gtctgttttc ctggatgcca tatcaaccac ctgacgccac
     7321 gtacgctgga tattgaccgg gtgcagtcga tgatgcctga atgcggaatt gaacccaaaa
     7381 ttctcatcga ggggccgccg cgccgcgagg taccgatttt actacgccag accagcttta
     7441 aagcactgga agagacggtg ttgtttgcgg ggcagaaaca gggcacgcat accgcgcgct
     7501 ttggtgaaat tgagcagcgt ggcgtggcat taacgccgaa agggcgacaa ctgtatgatg
     7561 atcttctgcg taacgctgga accgggcagg ataatctcac tcaccaaatg catttacagg
     7621 aaaccttccg cacttttcct gacagtgagt ttttaatgcg tcagcaaggg ttggcatggt
     7681 tccggtaccg tctgacgcct tcgggtgagg cgcatcgtca ggcgattcat cctggagacg
     7741 atccacagcc cttaattgaa cgtggttggg tagtggcgca acccatcacc tatgaagatt
     7801 tcttgcccgt tagcgcggcg gggatcttcc agtcaaatct gggtaatgaa acgcagacac
     7861 gcagtcacgg taatgccagt cgcgaagcat ttgagcaggc gttgggttgt ccggttttgg
     7921 atgagttcca gctttaccag gaagcggaag aacgcagtaa acgtcgctgt ggtttgcttt
     7981 aaattctgac catccgcctt tgcaaaaatt tgcctgattt tacaaacgaa tcaggctcat
     8041 cccatcgaca taaaaaaaat gccgatttat gcatattctc tcagttcaac aattggatta
     8101 ttaataaata ttgtctagag tgagcggtca taaataagca ctttcttgcc gctgaaaacg
     8161 accagcgcgg gaccattcac aacaccagaa ggactcactt tcaggtatgg atcgtagacg
     8221 atttattaaa ggttcaatgg ctatggccgc cgtgtgcggt accagcggca ttgcttctct
     8281 tttttctcag gcggcattcg cggcagattc tgatattgcc gacgggcaaa cccagcgttt
     8341 tgacttctcc attctacagt caatggcgca cgacttagcg caaacagcgt ggcgtggtgc
     8401 gcctcgtccg ttacctgaca cgctggcgac aatgacgccg caggcttata acagtattca
     8461 atacgacgcc gaaaaatcgc tctggcataa cgttgagaac cgtcaactgg acgctcagtt
     8521 cttccatatg ggaatgggat tccgtcgccg cgttcgtatg ttttctgtag atccagcaac
     8581 acatctggcg cgtgaaattc actttcgccc ggagttgttc aaatacaacg atgcaggtgt
     8641 tgataccaaa caattagaag ggcaaagcga tctcggcttt gccggttttc gcgtgtttaa
     8701 agcccccgaa ctggcgcgcc gtgatgtagt atcatttctc ggcgcgagtt atttccgcgc
     8761 cgttgatgat acatatcaat acggtttgtc ggcccgcggc ctggcgatcg acacttacac
     8821 cgacagtaaa gaagagttcc ccgactttac cgccttctgg tttgatacgg taaaaccggg
     8881 ggcaactacc tttaccgttt atgcgttgct cgatagcgcc agtattactg gtgcctataa
     8941 gttcactatc cattgtgaga aaagtcaggt gattatggat gtggaaaatc acctgtatgc
     9001 gctcaaagac attaaacagc tgggcattgc gccgatgacc agtatgttca gctgcggtac
     9061 taatgaacgt cggatgtgcg acaccattca tccgcaaatt catgactctg atcgtctgtc
     9121 catgtggcgg ggcaacggcg agtggatttg ccgtccgctg aacaatccgc aaaaattgca
     9181 gttcaatgct tacaccgaca acaacccgaa agggtttggt ttattgcaac tggatcgtga
     9241 cttctcccat tatcaggaca ttatgggctg gtataacaaa cgcccaagtt tgtgggtgga
     9301 accgcgtaac aagtggggta agggcaccat cggcctgatg gaaatcccaa caacgggcga
     9361 aacgctggat aacattgtct gcttctggca gccagaaaaa gctgtaaaag caggtgatga
     9421 gtttgcattc cagtatcgtc tgtactggag tgcgcaaccg cctgttcatt gcccattagc
     9481 gcgcgttatg gcgacgcgta ccggcatggg cggtttcccg gaaggttggg cgccaggtga
     9541 acactatccc gaaaaatggg cgcgtcgttt tgccgtcgat ttcgttggtg gtgatctgaa
     9601 agctgccgcg ccaaaaggca ttgagccggt gattacgctt tccagtgggg aagcgaagca
     9661 aatcgaaatt ctctatattg aacccatcga tggttatcgt attcagtttg actggtatcc
     9721 gacttcggac tctactgatc cggtcgatat gcggatgtat ctacgttgtc agggggacgc
     9781 tatcagtgaa acatggctgt atcagtattt cccgccagcg ccggataaac gtcagtatgt
     9841 tgacgaccgc gtgatgagtt aatcgttttt tcttcggcac cttcttcggg aggtgccgtc
     9901 tggttaaaca cgatcccgct cgcatttttc cctaagttaa atgagtaatc tgatggtgtg
     9961 tatttcagat acaccttgtc agccactaac agggagtgcg tatgtttcca gaataccgag
    10021 atttaatatc ccgtctgaaa aacgaaaatc ctcgctttat gtccttgttc gataaacaca
    10081 ataaacttga tcatgaaatt gccagaaagg aaggttccga cggtcgaggg tacaatgcgg
    10141 aagtggtccg catgaaaaaa caaaagctac agttaaaaga tgagatgctc aaaatcctgc
    10201 agcaggagag cgtcaaagag gtgtaaactt tcctaagccg ccagccaggc ggctttttta
    10261 acaacagcat ggattgactg gagataagat gactgaaacg ataaaagtaa gcgaatcact
    10321 tgaattacat gctgttgcgg aaagtcacgt cacacctctt tatcagttaa tctgtaaaaa
    10381 taaaacctgg ttacagcagt cgctaaactg gccgcagttc gttcagagtg aagaggacac
    10441 gcgaaaaacg gtgcagggta atgtgatgtt gcatcaacgc ggctatgcca aaatgttcat
    10501 gattttcaaa gaagatgaac ttatcggcgt tatctcgttt aatcgtattg aaccactgaa
    10561 taaaactgct gaaatcggct actggctgga cgaatctcat caggggcagg ggatcatttc
    10621 tcaggcgctg caggcattga ttcatcatta cgctcagtct ggtgaactta gacgcttcgt
    10681 gatcaaatgt cgggtggaca atccgcaaag caaccaggtc gctttgcgca atggttttat
    10741 ccttgaaggt tgcctgaaac aggctgagtt cctgaatgat gcctatgatg atgtgaactt
    10801 atacgcgcgt attatcgatt cataataacc ctgccagcgg cgttcgcgta atgcgctcct
    10861 cgccgttaat gacttttgga ccacggacat gaaccgtgtc gccatcaaag gcttcaacga
    10921 cggcgtggtc ggttaactcc acatggtttt caattatcac cgcacctgaa atacggctct
    10981 caccttgtat tacaacgtgc tcgtcgagca gaatcggtcc accacgtacc acggcatttc
    11041 cgccaatcaa gacatgatgt tttaacacac aattaccttc cacaatggca tattccgcca
    11101 cctgcgaact gtaatgaatc gtcggaatgg catcttcttc tatgccagct ttcacctgcg
    11161 cgtgaccgta gacttttgcg caatcgcata gccagacgtt gttctcttca ttaccttcaa
    11221 cactggcaaa atcaaaaact tcggcgcgat gttcaataaa agcataccgg acgacggcat
    11281 cgccataaat ttgtgcctgg tggacaatac gcgaggcgct aacccttgcg cgatcataaa
    11341 tttggagcag gagttgatgg tcgggcgtta agccttgtgc ggcgacgatc atagaatgtt
    11401 gatcaattaa ggcgtgacca aatattctac attgcccgta aactaatgag tcacgaatgg
    11461 tgacactgtc acttatatat gcaccctggc tgatttcact attatcgatc cagacgttat
    11521 ccgttgcgta aacctctccc cacagcacgc tggtgccggt gatacgcgta ttaccagaaa
    11581 tcaccgttcc gccgaacgca atagcattct ggtcgtaaat ccagcaattt ccttcctgtg
    11641 ctaacaccgt ttcgcgatcg atccagccgc cagcggttcc tgctatcaca tcgttaaaat
    11701 cgctaatggc gataatctgc cgtaataaca cacttttttt agtgccatct tcctgataac
    11761 taaaggctcg ctgttcttca cttagacgat atttgcgcat agcgttttct cacaggtgac
    11821 ttactataac cgtagcaaat tctgcggctc tggctatgct cgagaaaatt ccataaaatg
    11881 catttcaaat atactttata aattaaacaa aatgagtaag aagatgcaga gcgataaagt
    11941 gctcaatttg ccggcaggct actttggtat tgtgttgggg acgataggga tgggatttgc
    12001 ctggcgctat gccagccagg tttggcaggt cagccactgg ttaggggatg ggctggtgat
    12061 tctggcgatg atcatctggg gattattaac tagcgcattt attacccgac tcatacgctt
    12121 tccgcatagc gtgctggcgg aagttcgcca tccagtgctg agcagttttg tgagtttgtt
    12181 tccggcaacg acgatgctgg tggcgattgg ttttgttccg tggtttcgcc cactggcggt
    12241 gtgcctgttc agttttggtg tcgtggttca gttggcttat gccgcctggc aaactgcggg
    12301 attatggcgc ggatctcacc ctgaagaagc taccacgcct ggactgtatc tgccgacagt
    12361 tgccaacaac tttatcagcg caatggcctg tggtgcgttg ggctacaccg acgccggtct
    12421 ggtgttttta ggcgcaggcg ttttctcatg gctaagtctt gaaccggtga tcttgcagcg
    12481 tctgcgcagt tcgggagaat tacccacggc actgcggaca tcactcggca ttcagctcgc
    12541 tcctgcgctg gtggcctgta gtgcctggtt gagcgtcaac ggcggcgagg gtgatacgct
    12601 ggcgaaaatg ctttttggtt atggactgct gcaactgctg tttatgctac gtctgatgcc
    12661 atggtatctc tcccagccat ttaatgcttc attctggagt ttctcgttcg gcgtatctgc
    12721 actggcaacc accggtttgc atctggggag tggcagcgaa aatggatttt tccatacgct
    12781 ggcggtaccg ctgtttatct ttaccaattt tattattgca atactgctca tccgtacttt
    12841 tgcgcttctg atgcagggaa aattgttagt cagaaccgag cgcgccgttt taatgaaagc
    12901 agaggacaaa gaatgatcat tcgtgacgaa aactatttta ctgataaata tgaattaacc
    12961 cgcacacact ctgaagtact ggaagcggtg aaagtggtta aaccgggtaa aacgctggat
    13021 ctgggctgtg gcaatggtcg taacagtctt tacctggcag ccaatggtta tgatgttgac
    13081 gcatgggata aaaatgccat gagtatcgcc aatgtagagc gcattaaatc cattgaaaat
    13141 ctggataatt tacacacccg agtcgttgat ctgaataacc tcacatttga tagacagtac
    13201 gattttattc tttcgactgt ggtactgatg ttccttgagg ctaaaaccat cccagggctg
    13261 attgccaata tgcaacgttg cactaaacct ggtggttaca acctgattgt ggcggcgatg
    13321 gataccgctg attatccatg taccgtcggc ttcccgtttg ccttcaaaga gggagaatta
    13381 cgtcgatatt acgaaggctg ggagagggtg aaatacaatg aagacgtcgg cgagctgcac
    13441 cgcaccgacg ccaacggtaa tcgtattaaa ctgcgtttcg ccacgatgct ggcacgtaaa
    13501 aaatgacccg ctaagaataa ggcgcgtgaa aattccccac gctgagatga tttactgttc
    13561 ttcttttcgg taagcatatt ttttatcgaa gggatgtgaa attaatcaca gtagtcgaag
    13621 ttttttagca gcttaactta ctgaaattta actactgatg attgacttag cccctttttc
    13681 ggcattgact atgtcgtctg aaaaggggct gaaaaattta ttttcaccaa cacttttttt
    13741 gccacaacac gaagcggcgc tttttgctat aacttaaaaa gtaatataat catc
//
LOCUS       DAEEKQ010000105        13759 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.105, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000105 DAEEKQ010000000
VERSION     DAEEKQ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13759
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.105"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            254..2020
                     /gene="adeD"
                     /locus_tag="KLK57_002862"
     CDS             254..2020
                     /gene="adeD"
                     /locus_tag="KLK57_002862"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HBE0893177.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     GGEISGPIVIKGRYIAGVGAEYADAPALQRIDARGATAVPRFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGL
                     GGKELNAYITAGIENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSHSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDVEKFAFTTLEVTE"
     gene            complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="KLK57_002863"
     CDS             complement(2065..3456)
                     /gene="uhpT"
                     /locus_tag="KLK57_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HBE0893178.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="KLK57_002864"
     CDS             complement(3594..4913)
                     /gene="uhpC"
                     /locus_tag="KLK57_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HBE0893179.1"
                     /translation="MLPFLKAPADAPLMADKYEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="KLK57_002865"
     CDS             complement(4923..6425)
                     /gene="uhpB"
                     /locus_tag="KLK57_002865"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HBE0893180.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLVLP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="KLK57_002866"
     CDS             complement(6425..7015)
                     /gene="uhpA"
                     /locus_tag="KLK57_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HBE0893181.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="KLK57_002867"
     CDS             complement(7090..7380)
                     /gene="ilvN"
                     /locus_tag="KLK57_002867"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBE0893182.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="KLK57_002868"
     CDS             complement(7384..9072)
                     /gene="ilvB"
                     /locus_tag="KLK57_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HBE0893183.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIEAQPAVAEKAAAPAFSEESIRDVATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPSPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="KLK57_002869"
     CDS             complement(9178..9276)
                     /gene="ivbL"
                     /locus_tag="KLK57_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HBE0893184.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            9841..9930
                     /gene="tisB"
                     /locus_tag="KLK57_002870"
     CDS             9841..9930
                     /gene="tisB"
                     /locus_tag="KLK57_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HBE0893185.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            10210..11394
                     /gene="emrD"
                     /locus_tag="KLK57_002871"
     CDS             10210..11394
                     /gene="emrD"
                     /locus_tag="KLK57_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HBE0893186.1"
                     /translation="MKRHRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(11402..11899)
                     /locus_tag="KLK57_002872"
     CDS             complement(11402..11899)
                     /locus_tag="KLK57_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HBE0893187.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRCDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(11896..12258)
                     /locus_tag="KLK57_002873"
     CDS             complement(11896..12258)
                     /locus_tag="KLK57_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HBE0893188.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(12248..12595)
                     /locus_tag="KLK57_002874"
     CDS             complement(12248..12595)
                     /locus_tag="KLK57_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HBE0893189.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            12704..13153
                     /locus_tag="KLK57_002875"
     CDS             12704..13153
                     /locus_tag="KLK57_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893190.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFVGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(13402..>13759)
                     /locus_tag="KLK57_002876"
     CDS             complement(13402..>13759)
                     /locus_tag="KLK57_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0893191.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLAYKQLWRGGQVLAVPPA
                     YTSQRCVCCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQS
                     GRSLKQEPTEMIQATA"
BASE COUNT         3267 a         3817 c         3568 g         3107 t
ORIGIN      
        1 tcccatgctg aggtagttta aataatcgcg aaagcgtccc tgattcatta ctcacgtaat
       61 cggtattgtc attattcatt tttttgtcca ttggaggaga tttaatccct tgcttttgta
      121 aaacaaatga caacaacggt tcagtgataa ttatcacatt tcaattgcac attaatggat
      181 attctttaat aatctcgcga cgtttcttta tgataaataa taatcaaatt gataaaatca
      241 aaatgagaaa aatatgaata attctattaa ccataaattt catcacatta gccgggctga
      301 ataccaggaa ttgttagccg tttcccgtgg cgacgctgtt gccgattata ttattgataa
      361 tgtctctatt ctcgacctga tcaatggcgg agaaatttcc ggcccaattg tgattaaagg
      421 acgttacatt gccggtgttg gcgcagaata cgctgatgct ccggctttgc agcggattga
      481 tgctcgcggc gcaacggcgg tgccaaggtt tattgatgct cacctgcata ttgaatccag
      541 catgatgacg ccggtcactt ttgaaaccgc taccctgccg cgcggcctga cgaccgttat
      601 ttgcgaccct catgaaatcg tcaacgtgat gggagaagcc ggattcgcct ggtttgcccg
      661 ctgtgccgaa caggcaaggc aaaaccagta cttacaggtc agctcttgcg tacccgccct
      721 ggaaggctgc gatgttaacg gtgccagttt tacccttgaa cagatgctcg cctggcggga
      781 ccatccgcag gttaccggcc ttgcagaaat gatggactac cctggcgtaa ttagcgggca
      841 gaatgcgctg ctcgataaac tggatgcatt tcgccacctg acgctggacg gtcactgccc
      901 gggtttgggt ggtaaagaac ttaacgccta tattactgcg ggtattgaaa actgccacga
      961 aagttatcag ctggaagaag gacgccggaa attacaactc ggcatgtcgt tgatgatccg
     1021 cgaagggtcc gctgcccgca atctcaacgc gctggcaccg ttgatcaacg aatttaacag
     1081 cccgcaatgc atgctctgta ccgatgaccg taacccgtgg gagatcgccc atgaaggaca
     1141 catcgatgcc ttaattcgcc gcctgatcga acaacacaat gtgccgctgc atgtggcata
     1201 tcgcgtcgcc agctggtcga cggcgcgcca ctttggtctg aatcacctcg gcttactggc
     1261 acccggcaag caggccgata tcgtcctgtt gagcgatgcg cgtaaggtca cggtgcagca
     1321 ggtactggtg aaaggcgagc cgattgatgc gcaaacctta caggcggaag agtcggcgag
     1381 actggcacaa tccgctccgc catatggcaa caccattgcc cgccagccag tttccgccag
     1441 cgactttgcc ctgcaattta cgcccggaaa acgctatcgg gtcattgacg tcatccataa
     1501 cgaattgatt acgcactccc actccagcgt ctacagcgaa aatggttttg atcgcgatga
     1561 tgtgtgcttt attgccgtac ttgagcgtta cgggcaacgg ctggctccgg cttgtggttt
     1621 gcttggcggc tttggactga atgaaggtgc gctggctgcg acggtcagcc atgacagcca
     1681 taatattgtg gtgatcggtc gcagtgccga agagatggcg ctggcggtca atcaggtgat
     1741 tcaggatggc ggcgggctgt gcgtggtacg taacggccag gtccaaagtc atctgccgtt
     1801 gcccattgcc ggcctgatga gcaccgacac ggcgcagtcg ctggcggaac aaattgacgc
     1861 cttgaaagcc gccgcccgtg aatgcggtcc gttacccgat gagccgttta ttcagatggc
     1921 gtttctttct ctgccagtga tccccgcgct aaaactaacc agtcaggggc tatttgatgt
     1981 cgagaagttt gccttcacta cgctggaagt cacggaataa taaaaaagcc cggcgtcatg
     2041 ccgggcaaaa gtcaccagtt acgtttatgc cactgtcaac tgctgaattt ttttctcgcg
     2101 gcggattttg cgttcttcca tcaccgccac tatcgccatc aggcagatac aaccaatcgc
     2161 ggcgatatca agtgcggcga aggtgcctgc ccagccggta aggccgaata ccggcgtccc
     2221 atcggcaatc attcccagac ctaacttggc aaagctgtca ccaatcaggt aagcaaaggt
     2281 gcctttaata ccatcggcag cgccaatcgc ttttttaggt acaaagccaa cagcagccac
     2341 accaatcaac aattgcgggc caaagaccag gaaacccaac gcaaagagag aagccagata
     2401 gatatattcg ttactggcat gttgatacac accgagcgtg gcgataatca gcgccagcgc
     2461 gatgcaggcc accaggccac ggcgaccgtt cgccaggtca gagagccagc cccacagcag
     2521 cgtaccgacc agcgcaccag cttcaaacag cgtaaagccc tgaatcgcca ccgctttaga
     2581 gagtttcagt tcctggaacg cgtatacggt tgaccactgg tcgataccaa tacgtaccac
     2641 atagaggaaa atgttggcga agcacagcag ccagatcact ttgtttttca gcacatactc
     2701 aacaaagatc tgccacttgg tcatatcggt agattctgtc tctttgtcct cttcgctgat
     2761 ctcctcgccg aacagttctt cagctttgcc gaggccataa gattccgggg agtcgctgcc
     2821 gtaacgcagg ccgataaaac cgacaatcag cgcgataatc gacgggaaga taaacatgcc
     2881 gatgacatgg ccatcgaaca ggtaatttgc cccgaacagc gccacgcctg ctgcgcctgc
     2941 accgccaagg ttgtgagaaa tattccagaa accgaggaat gtcccgcgtt tacgacgcgg
     3001 cgtccatttg gtgatggtgg agtaactgca cgaaccgccg gtactctgga aaaagccgct
     3061 taaggcgtag aaggcaatca tcaggaacag gctaaccgag ccgctgccca tactggcact
     3121 gaagcccagc atacaaatgg cagacaggat cagcatgaac ggcaggaatt gtttggtgtt
     3181 tttgccgtcg gcgtagtagg aaaccagcgt tttacccacg ccataagtga tggagaaacc
     3241 caggccgatc atccccagct gcgtcatgct caacccgtag gtcgagatca tgtcgttctg
     3301 cgcgatgtta aagttcttgc gaatcaggta catcgtcagg tagccgataa agaccaccag
     3361 gtaggattgc atgaacggtt tgaaccacat tttgcgccgc acttcgagcg gaaggtccag
     3421 ggtcggcttg cgaacctggt ttaagaaagc cagcatgggt tactcctgaa atgaatacct
     3481 gcgtgaggca tgcattgtaa aaatcagcag caaatcaggc ctgagatagc gtccaggcaa
     3541 aacctggaaa atttcttagt tttgccggat atgaagtgaa aaggtgagat gcatcacgct
     3601 tcgcgcggtg tctgggcgtt caaaaagggc agtaacagca gtgcggaaat cccggcggcg
     3661 atagagataa ccacaaaaaa tccgctccag tgccaggtat cgagtacttt cgccagcggc
     3721 caaccagcaa gcgacgcccc cagataagca aacaagccga caaaccccgt cgccgcccct
     3781 gccgcctctt tgtgggaaca ctctgccgcc gccataccga ttaacatctg tgggccaaag
     3841 acaaaaaaac caatggtgaa gaagcaggtt gcctgcatca cgtagctggc aaatggcatc
     3901 agccacaggg agccgactga aagcaaaatt ccggcggcga aaatcaaatt catcggccct
     3961 cggttgccgt taaacaattt gtccgagccc caacccgcta ccagcgcacc gataaatcca
     4021 cccagttcaa acatcgtcac tgccgtattc gccgtgacca gatcgacgcc cagcgtctcg
     4081 gacatataca aattgcccca gtcgttgatc gccgcccgga ccacatagac cagcacatag
     4141 caaaacgaaa gcagccagat atacggattc agcaacacat atttggtgag gatctcttta
     4201 cgcgtcagcc ctgccccttc ttgttgttga gctatctcca gcgcgtcgtg tcgccattca
     4261 ccgaccgccg gtaaacctaa cgcctgcggg cgatcgcgca gccgccagca gagaaaaatt
     4321 cccacgacga tcgccataca accagcaatc atcatcccgg cacgccagcc gtaatgtagt
     4381 gcagccgctg ccatcacaat gggaatgagt gcgccgccga cgttatgcgc cgtgttccat
     4441 aatgcccacc aaccaccgcg ctcggtacgt gaataccagg ccgttaacag acgcgcacac
     4501 accggtgaac cccagccctg gaaaaaggcg ttcagcaccc agagtacggc aaacgcccat
     4561 agcgacgtcg agaagccaaa cagaatgttg ataatgcccg tggcgataag ccctatcccc
     4621 ataaaataac gggcatttga gcgatcgctg acaatgccgg agacaaactt cgacacgcca
     4681 taggtaatgt aaaacagggt cgctaacagg ccgatatcgc tacggctgag cacgccgtta
     4741 gcaaggattt ctggtacggc ggcgttaaaa cttttccgcg tgaagtaaaa cagcgcgtaa
     4801 cccagccaga tggtcagcag aatatgccga cgccagtagc gatagcgggc atcaatttca
     4861 tatttatcag ccattaatgg cgcatcggca ggcgctttca gaaacggcaa catcatcaaa
     4921 ccttagacat agcgttgagg tagagaaacg ctgacacgcg tgccgtgcag acaggaaatg
     4981 tgtaatgtgc cacccagcgc cgttacgcgc tcgcgcattc cggtgaggcc aaaaccttgt
     5041 tgcccggaac ccggcggcaa accgctgcca tcgtcttcaa taaccagcat caaccgttca
     5101 tcctgctgcc agccttgcag ggtgaccgcg ctggcatcag catgtttcac aatgttgttc
     5161 agcccttcct ggcagacacg aaacagcgtc acgcgctggt tttcgcttaa cgctgattca
     5221 tcgattcgcc attcgagatg gctgacaata ccgcgccctt ccagctccat ttcccgcatc
     5281 agtgagcgga tggcctgctc cagggtgaga tcatccaact ggcgcggacg taaccgaccc
     5341 aacaaacggc gcaccgcgtc gtaaacgccc agcgatagtt gttcgatgag ttgcccgctc
     5401 tgcttcacgc tggcgttatc tgccgccagc cgctgaacaa tgcccgcctg agtacgaata
     5461 gcagtgatgg tctggccgat atcatcatgc agctcacgcg ccacatcacg gcgcacgctc
     5521 tcttcggttt ccagcaaccg ttcagccaga tgctgattgc gcgccagttc cttttgcagc
     5581 gactggttaa gttcacgcaa ccgctggatg ccagcgccaa gcaacaaccc tgtcagactt
     5641 tgcaccagca gcgagagcaa taaatccacc ggatgatcgc gccaggtttg actggcgatc
     5701 agcgcgatgg cgttcatcaa cgtcgcaatc agcgcccctt gccaaccata gtgccaggcc
     5761 agcgcgataa tcggcagcac cagacagaat ggcgtaaagc gcgacagttc gtccggcaat
     5821 cccaactgga gccagagact gataacaaac agcagcaagt accagaccag atgtcgcccg
     5881 cgccagttga ttggctgaga aaccagtgac gggccgagcg gcagccaggt gttattggcg
     5941 agatagtgcc agaacaccag acatatcggg gccagcgtca ggccgccagt taaagtcagc
     6001 aacagcgcat tccacgactc tttgccgtgc caaagccagg gcagcgactg caacaacgcc
     6061 gccgccgtta acgccgcccc ctgtaacagc aaggtgcgcc agtcacgctg atggcgatag
     6121 cgcgagatca gcgctacggg cagtaacgtc agtaaactac cgatcatcaa taacggaaaa
     6181 tgggttaaac cgaccgcctg cgttagccag taaatcagca gccactccgc acccagcaat
     6241 acgggccagt atccgcgcgg gcattgcagc attagcccca gacgcagacc aaacggaaat
     6301 aacagcaccg ccatatcagg gcgctcaacc agatgcaggc tgatactcca caggcaaaac
     6361 catgcggcag agaagataaa aaagcaggca ataacggtaa ttaagcggga gaacaacgtc
     6421 ttcatcacca gccatcaaac atgcggcgcg ccagctctac gtcgttactg acgcccagtt
     6481 tttccatcag attggcgcga tggacgtgta ccgttttcgg tgacaagccc agttcggcgg
     6541 caatctcttt caccgccatt ccttgcgcca gtttttccgc cacctggcgt tcacgtttgg
     6601 ttagcgggtc ctgacgaccg gatgccagtt taatggcaat atccggcgtc agataacagc
     6661 cacccgtggc aaccgtatgc accgcagcaa tgagttcatc cgggctacag cgtttggaaa
     6721 gaaagccgcg tgcccccgcg ttaagcgcct gctcaaccag cgcaggactg tcgtgaacgg
     6781 agagcataat cgtcgccata cctttcggca gctggcttag cagctccaga ccggagatat
     6841 cgggcatgga gatatcgcaa atacacacct gcacaccgcg ccccggcagc cccgccagcg
     6901 cctcgcgccc cgaaccaaac tcggcaacta cctgcaaatc aggttccagc cccagcagct
     6961 gcgcaaagcc ggagcggacg atgaggtgat cgtctataag ggcaacggtg atcatggtct
     7021 tgtcctggcg ggtaaaaaaa aacgcgctta ccttaacgat aagcgcgatg ttgttcaagc
     7081 cttgagcggt tactgaaaaa acaccgcgat cttgttaaac atcgtcggat cggactgatt
     7141 acgctgcact ttcacgacat cttccagctt atcgatttgg cttatcatct gctccagacg
     7201 ctggtcgtca ttgaccagta gccagatatg gcttttgtcg ctgtcctgaa tcggcagaca
     7261 aagaatgcct tcaacgttaa aagcgcggcg ggcaaaaagg ccacaaacgt gggtcattac
     7321 gcccggatgg ttgcgaacgg tgagctccag aattacgttg tcatgagttg tgttttgcat
     7381 ggcttattcc cccaccattt cagtattcgc cgcacctggc ggcaccatcg ggtaaacttt
     7441 ttcttcggca tcaatgcgca catggatcag cgccgggcta gggcgattga tgatttcctg
     7501 caatgcagcc tgcggatcgg cttcgttatt caaatcacag gtttcgaggc cgaatccggc
     7561 ggcaatctgc ataaagttga ttttgcccgg ataggtggcg gcaaaaacgc cttgctcgta
     7621 gaacagactc tgttgctgat gcaccagccc cagcgcttcg ttgttcatca gaatgatttt
     7681 gacatccagc tgattttcac tggcggtcgc catctcctga atattcatca tcaggctgcc
     7741 gtcgccggag aaacacaaca ctttgcgatc cgggttcgcc agcgccgcgc caatcgccgc
     7801 aggcaggcca aaacccatcg tgcccagccc accggaggtc agccactggc gtgggcgatt
     7861 gagcggataa gcttgcgcgg tccacatctg atgctgaccc acatcggtgg tgataatcgc
     7921 attgtcatcg acacaggcgg caacggcgtt gatcaggccg taatggctta atggatcgca
     7981 cgctttcggg attggacacg ggaattcgcg ctgcaaatcc gctaccaact ggtgccactc
     8041 tgcacgcggt tgcgcttcca cctgcgggat caattgcgcc agcacgtcat caacatccgc
     8101 ctgaatcgcc acatgcggct gcttgatttt acccagctct gcgcgatcga tatcgacatg
     8161 aataattttg gcattcggac agaactgctc ggttttgcca atcgcccggt catcaaaacg
     8221 cgcaccgagc acaatcaaca aatccgcctc ctgcaaaata tagttggtac tgcgcacgcc
     8281 gtgcatcccc agcataccca gcgacaacgg atgcgctttt ggcagcatgc ccagcgccat
     8341 taaagtcatg gtggtaggca gttgcgcttt ctccgccagt tcacgcaccc gcgcaggcgc
     8401 attgatcaca ccaccaccca gataaagcac cgggcgtttg gcagcgttaa tcattgtagc
     8461 tacgtcacga atgctttctt cgctaaaggc gggtgcagcg gctttttctg ccaccgcggg
     8521 ctgagcttca atctcaaaaa ccgccgtttg tacatcctta ggaatgtcta tccacaccgg
     8581 gccagggcgg cctgattgcg caatgcggaa ggcatcgctc atgacctgcg ggagttcttc
     8641 gatatgtctg accagatagt tgtgtttggt gatggggata gagatgccgt aggtgtcgac
     8701 ttcctggaag gcgtcggtgc cgatcatcga ggcgggaacc tgaccagtga tgcaaatcag
     8761 cgggatggag tccagccgcg catcggcaat ggcggtcacc aggttagtcg cacccggtcc
     8821 gctacaggcc atacagaccg ccggtttacc gtcggtgcgc gccattccct gagcgataaa
     8881 gcccgcgccc tgttcatgac gggccagaat atggcggatt tgcgtgcttt ggcttaaggc
     8941 atcgtaaaca ggcaggatag aaccgcccgg aatgcctgtc acaatcttaa tgccctgctg
     9001 ttccaggaaa tgaacgataa attctgcgcc ggtaaagcgc ttacgcgtca atgttgtgcc
     9061 cgaacttgcc atgctccagt ccttttcttc tgggccgact ttccgggagg tgcttaaacg
     9121 aaaaaccccg cccggtttgc gccgacgggg ttttggaatc gtgtgttgtt ccagtcccta
     9181 cggcgcattg ccgacgacca ccaccacacg cacgacgacc actgcggcgg atggcgcagt
     9241 tggtagtagt tttgcgttga acatggaagt agtcattggg gaccttgttg gttttgtgtt
     9301 taacaatatt tatacaagca cagctttaca ggggagacaa tggaaaattt ttcagcaagg
     9361 gaaaattgag gggttgatca cgttttgtac tgaattgcag ataacaaaaa accccgccgg
     9421 agcgaggttt cgtcagtcgc ccgcggctgg taaccgcaaa gcacactgta ttatgtcaac
     9481 acagaaagta tacgtgttcc gcgcagaacg cgcaatttcg gcacgaattt tgacgtattt
     9541 agtgcatagt tgagtatcga tcacagtttg cgttttgtcc aaatattgct gtttatttat
     9601 acagtaaact tctataatat cactgtacgc aatgtgttat gcgggggccg catcgttacc
     9661 cggcgcacta agtcctggct gaaacgggtg gtgccgtcag cgccttaacc ccgcgtgagc
     9721 acactgtgtt atgtcaacaa gcacaaagtt tctccttgag ataccgcgtg cacaacagct
     9781 ggcaacaggc agcggaaagg tacgtcagct ggcagtgctc ctgaaccaca ggagacgcgt
     9841 atgagcctgg tggatatcgc cattcttatc ctcaaactca ttgttgcagc actgcaactg
     9901 cttgatgctg ttctgaaata cctgaagtaa ttcagattca agtcgcacca aaggggagcg
     9961 ggtaaccgct ccccttttat atttagcgtg cgggttggtg tcggatgcga tgctgacgta
    10021 tcttatccgc cctaccatct ctcccggcaa catttattgc cgcttttgtt tacatattct
    10081 gccgctaaac aactccccat tcctggcgta tatctggcta acattcatca atgtgataga
    10141 ttcctctccc gcatttatgg gaatgcgtag tgacttactc taattatttt tataaaagca
    10201 tccgtgataa tgaaaaggca tagaaacgtc aatttgttat tgatgttggt attactcgtg
    10261 gccgtcggtc agatggcgca aaccatttat attccagcta ttgccgatat ggcgcgcgat
    10321 ctcaacgtcc gtgaaggggc ggtgcagagc gtaatgggcg cttatctgct gacttacggt
    10381 gtctcacagc tgttttatgg cccgatttcc gaccgtgtgg gtcgccgacc ggtgatcctc
    10441 gtcggaatgt ccatttttat gctggcaacg ctggtcgcgg tcacgacctc cagtttgacg
    10501 gtattgattg ccgccagcgc gatgcagggg atgggcaccg gcgttggcgg cgtaatggcg
    10561 cgtactttgc cgcgagattt atatgaacgg acacagttgc gccatgctaa cagcctgtta
    10621 aacatgggga ttctcgtcag tccgttgctc gcaccgctaa tcggcggtct gctggatacg
    10681 atgtggaact ggcgcgcctg ttatctcttt ttgttggttc tttgtgctgg tgtgaccttc
    10741 agtatggccc gctggatgcc ggaaacgcgt ccggtcgatg caccgcgcac gcgcctgctt
    10801 accagttata aaacgctttt cggtaacagc ggttttaact gttatttgct gatgctgatt
    10861 ggcggtctgg ccgggattgc cgcctttgaa gcctgctccg gcgtgctgat gggcgcggtg
    10921 ttagggctga gcagtatgac ggtcagtatt ttgtttattc tgccgattcc agcagcgttt
    10981 tttggcgcat ggtttgccgg acgtcccaat aaacgcttct ccacgttaat gtggcagtcg
    11041 gttatctgct gcctgctggc tggcttgctg atgtggatcc ccgactggtt tggcgtgatg
    11101 aatgtctgga cgctgctcgt tcccgccgcg ctgttctttt tcggtgccgg gatgctgttt
    11161 cctctggcga ccagcggcgc gatggagccg ttccccttcc tggcgggcac ggctggcgcg
    11221 ctggtcggcg gtctgcaaaa cattggttcc ggcgtgctgg cgtcgctctc tgcgatgttg
    11281 ccgcaaaccg gtcagggcag cctagggttg ttgatgacct taatgggatt gttgatcgtg
    11341 ctgtgctggc taccgctggc gacgcggatg tcgcatcagg ggcagcccgt ttaagcgcac
    11401 gtcaccgcag catcgtcatc agctccatgg gagaacgatg ctgctttatc agatcacgca
    11461 tcacccgcat atgcggtgcg gagtaagaat aaaacgcctg atagcccgca caaagcacgc
    11521 tgtctggcgt ctcctgccga gcgtgcgggc acaggcgatg ccagtcgcac gcctggcagt
    11581 cacagcgcgg ttgcgggttt tcgctgccac accatttttg taaggtggcg tcaaaaagct
    11641 caatcgagat acgactgata tctgcccgca cccaacactc gaacagggca attaaaaatt
    11701 tcccccacag ctctgctgtc accgaagcgg ccgtcagatc gccccggtca tcaaattcaa
    11761 gatagggaat gagctgtacg gagcggatgc cccgctgttt gaaattgtga taaaaatcgg
    11821 caggttgcag agcatccttg tactctacaa ccagtttatg cctgtcctct tcagcatcaa
    11881 tgacctgact tcctgtcatg cgactctctc aataaatacg ataagttgat gtatatgcgt
    11941 tacagcaaac agtattgcga gagataacac cgcgagggag atcaaaaatt tgtcacggac
    12001 tacgtgaaat tgggaaaaat cgctattcgt gacatccatt aaattgcgat tacgggtgta
    12061 gtgccagagg atcagcgcca cgatggcgag aatgccaatc gaaatccaga ataacatgcc
    12121 cgcctggtgc cagttgtgtt tgatagccaa cgccatcaac gcgccgtagc ccagcatggt
    12181 acgaaaccac gccagtgatg tgcgctccgg ctgcagtcct gggtcggcaa tgcgtctggc
    12241 tttgcggcta tccggcatac aacaccagtc ccataacgat caccgccacg accatcaata
    12301 ttaagctaat gattaataag ctgttggtgt agggcaggtc ctctttcaga cgcatcgctt
    12361 tttcattgcg taaccagcgc aggtagccat acattgctaa cccgccggaa aacagacaca
    12421 acaacaacgc cagcagttca cgaatgacgg gcgtggcgaa atctggcgca agttgatcaa
    12481 gcccaacccc cgccgccaga aatcctagtg cggtacggat ccacgccaga aaagtacgct
    12541 cattagccag cgagaagcgg taatcgggcg cttctccaag gcgggaaatt ttcattgttg
    12601 ctcctttgca atttcactaa ctctctacct ttagcggttt tttttaagaa tttcgtccgc
    12661 ccgtttcctg atatgctttt gccatccgct acaaggaggt cagatgggta tcatagcgca
    12721 aaataaaatc agttcattgg gcatgctgtt cggtgccatc gctttaatga tggggattat
    12781 ccacttttca ttcggccctt tttccgcacc accgccaacg cttgaaagca tcgtggcgga
    12841 caaaacggct gaaattaaaa gaggtcttct cgccggaata aaaggcgaaa aaattaccac
    12901 cgtggagaaa aaagaggacg tagatgtcga taaaatcctc gatcaaagcg gtatcgcact
    12961 ggccattgct gcgctacttt gcgcatttgt tggcggtatg cgtaaggaaa atcgctgggg
    13021 cattcgcggc gcgctggttt ttggcggcgg aacgctggct ttccatacct tgctttttgg
    13081 tatcggcatc gtgtgcagca ttttgctgat attccttatc ttttccttcc tgaccggcgg
    13141 ttcgctggtt taagcagcct gaataatgcc cgccgggtga tcatcagcgg gcattatcgt
    13201 cagaacttct gttttttctc ttccaccttc accccttgtg tcggtaggcc ggtgtcatag
    13261 tcacgtacca ccggcgaata gccatcttgt gggcgtggac gaaatgcccc catccagcgc
    13321 ggccgtgcat ctttacgcca cggacgcagg ctccattgtt gacatcctcc acgccctgaa
    13381 ggacgtggcc ccctgctacg ttcaggctgt cgcctgaatc atttcggtgg gttcctgctt
    13441 caacgagcgg cctgactgca ccatctctcc acaggcaaga acggcgtgcc ccgccgctaa
    13501 aatgttacga gcgccattta catcggcgtt cgctgtatat ccacatacct ggcatctgaa
    13561 tttactttgt gacaggcgat tttctttcgc tgtatgacca cagcacacgc aacgctggct
    13621 tgtgtacgct ggcggaacag caagcacctg accaccacgc cagagctgct tgtacgcaag
    13681 ctggcggcgc atttcatacc agccctgatc cagtatcgaa cggtttaaac ctgattttgc
    13741 ccggacattg cgccccggc
//
LOCUS       DAEEKQ010000106        13547 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.106, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000106 DAEEKQ010000000
VERSION     DAEEKQ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.106"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            79..2307
                     /gene="crfC"
                     /locus_tag="KLK57_002877"
     CDS             79..2307
                     /gene="crfC"
                     /locus_tag="KLK57_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HBE0893192.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELSKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGVHFPVFHGVVPHVNTRQLFSEVISRQDATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFCAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            2304..3170
                     /locus_tag="KLK57_002878"
     CDS             2304..3170
                     /locus_tag="KLK57_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HBE0893193.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSGGEHADFLSWLGKWSTADKQPVCWSVTQRWQTVALGMPRLCSAQRLVG
                     AMVEEIFSVNLA"
     gene            3434..4936
                     /gene="proP"
                     /locus_tag="KLK57_002879"
     CDS             3434..4936
                     /gene="proP"
                     /locus_tag="KLK57_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HBE0893194.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDDIDHEIADLQAKRTRLVQQHPRIDE"
     gene            5048..5137
                     /gene="pmrR"
                     /locus_tag="KLK57_002880"
     CDS             5048..5137
                     /gene="pmrR"
                     /locus_tag="KLK57_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HBE0893195.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATY"
     gene            complement(5113..6213)
                     /gene="pmrB"
                     /locus_tag="KLK57_002881"
     CDS             complement(5113..6213)
                     /gene="pmrB"
                     /locus_tag="KLK57_002881"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HBE0893196.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISIFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQYVANQI"
     gene            complement(6214..6882)
                     /gene="basR"
                     /locus_tag="KLK57_002882"
     CDS             complement(6214..6882)
                     /gene="basR"
                     /locus_tag="KLK57_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HBE0893197.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYTCDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            complement(6879..8522)
                     /gene="eptA"
                     /locus_tag="KLK57_002883"
     CDS             complement(6879..8522)
                     /gene="eptA"
                     /locus_tag="KLK57_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HBE0893198.1"
                     /translation="MLKRLLKSPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQQAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDVLQTCRRVSE"
     gene            complement(8626..9963)
                     /gene="adiC"
                     /locus_tag="KLK57_002884"
     CDS             complement(8626..9963)
                     /gene="adiC"
                     /locus_tag="KLK57_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HBE0893199.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            complement(10100..10861)
                     /gene="adiY"
                     /locus_tag="KLK57_002885"
     CDS             complement(10100..10861)
                     /gene="adiY"
                     /locus_tag="KLK57_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HBE0893200.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            complement(11186..13453)
                     /gene="adiA"
                     /locus_tag="KLK57_002886"
     CDS             complement(11186..13453)
                     /gene="adiA"
                     /locus_tag="KLK57_002886"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HBE0893201.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLSVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
BASE COUNT         3276 a         3310 c         3563 g         3398 t
ORIGIN      
        1 aaaacagaac tcaaacagaa ctcaaaaaca atctggctaa ataaaagtaa caaaatttgc
       61 tttaaggaag aattttctat gtacacacag accctgtatg agttaagtca ggaggctgaa
      121 cgcctgttac agctttctcg ccaacagttg cagttactgg aaaaaatgcc tctctctgta
      181 cccggagacg acgcgccaca actggcttta ccctggagtc agcctaatat cgccgaacgt
      241 cacgcgatgc tgaataatga gttgagtaaa atttcccgac tggaaatggt gctggcgatt
      301 gtcggtacta tgaaagcagg gaaatcaacc accattaatg ccattgttgg tacggaggtt
      361 ctgcctaatc gtaatcgccc aatgactgcg ctgccgacgc ttattcgcca tacgcccggg
      421 caaaaggaac cggtactgca tttttcacat gtcgcgccaa tcgattgttt aattcaacaa
      481 ttacaacagc gcctgcgtga ttgcgatatt aagcatctga ccgatgtgct ggaaatagat
      541 aaagatatgc gtgcgcttat gcagcggatc gaaaatggcg tcgctttcga aaaatattat
      601 ctgggtgccc agcctatttt tcattgtctg aaaagtttga atgatttagt gcgactggcg
      661 aaggcgctgg acgtcgattt tcctttttct gcttacgccg ccattgagca tattcccgtg
      721 attgaagtgg agtttgtcca tctggcgggg ctggagagtt atcccggtca gttgacgtta
      781 ctggataccc ccgggccaaa tgaagccggg caaccgcatc tgcaaaaaat gcttaaccag
      841 cagctggcac gcgcctcggc ggtactggcg gtgctggatt atacgcaact gaaatcgatc
      901 tccgatgaag aggtccgtga ggcgattttg gcggtggggc aatcggtgcc actgtatgtg
      961 ctggtcaata agttcgatca acaggatcgt aacagtgacg acgccgacca ggtgcgggca
     1021 ctgatttccg ggacgctgat gaaaggctgt attacgccac agcagatatt tccggtgtcg
     1081 tcgatgtggg gctacttggc aaatcgagcg cgccatgagt tagccaacaa cggtaagtta
     1141 ccagcgccag agcaacaacg ctgggtggaa gattttgccc atgccgcgct cggcaggcgc
     1201 tggcgtcatg ccgatctggc ggacctcgaa catattcgtc atgctgccga tcagttgtgg
     1261 gaagattcgc tgttcgccca gccaattcag gcgttgcttc atgccgctta cgctaacgcc
     1321 tcgttgtatg ctctgcgatc tgctgcgcat aaactgttga attacgcgca gcaggcgcgg
     1381 gaatacctgg attttcgtgc gcacgggtta aacgtcgctt gtgaacaatt gcggcaaaat
     1441 atccatcagg tcgaagaaag tttgcagcta ttgcaactca atcaggcgca ggtgagcggc
     1501 gagattaaac atgaaatcga gctggccctg acctccgcca accactttct gcgtcaacag
     1561 caagatgcgc tgaatgcgca gttagccgcc ttgtttcagg atgattcgga gccgttaagc
     1621 gagattcgta cctgctgtga gacactgtta cagacggcgc agaacaccat cagtcgcgac
     1681 tttacgctgc gttttgccga gcttgaatcc accctttgcc gggtgttaac cgatgttatt
     1741 cggcccattg agcaacaagt caaaatggaa ttgagcgagt cagggtttcg tcctggggtt
     1801 cattttcctg tttttcacgg agtagttccc cacgtcaaca ctcgccagct gttcagtgaa
     1861 gtcatttcgc gccaggatgc aacggacgag cagagcacgc gtttaggcgt tgtgcgtgag
     1921 actttttcgc gctggttgaa tcagcccgac tggggacggg gaaatgagaa atccccgaca
     1981 gagacggttg attacagtgt gttgcaacga gctttaagcg cagaagtcga tctttattgc
     2041 caacaaatgg ctaaagttct ggcagagcag gtcgatgaat ctgttacggc aggcatgaat
     2101 actttttgcg ctgagttcgc ttcatgtttg acggaattac agacgcgttt acgcgaaagc
     2161 ctggctctgc gtcaacaaaa tgaatcggtg gtcaggctga tgcagcagca attgcagcag
     2221 actgtgatga ctcacggctg gatttacacc gacgcccagc tgttacgcga tgatattcaa
     2281 acacttttca cggcagaacg atattgacca agacgttact ggacggcccc ggtcgcgtgc
     2341 tggagtcggt ttatccccgc tttttagtgg atctggcgca gggcgatgat gcccgccttc
     2401 cacaagccca tcagcagcag tttcgtgaac gactgatgca ggaacttctt tcgcgtgtgc
     2461 agcttcagac atggacgaac ggcggcatgt taaatgcgcc gcttagcctg cgactgacaa
     2521 tggtggaaaa actggcgtcg atgctggatc ccggtcatct ggcgctgacg cagatcgcgc
     2581 agcatctggc gctgctgcaa aaaatggatc accgccagca ctctgctttc ccggagctcc
     2641 cccagcaaat tgccgccttg tatgagtggt tttcagcccg ttgtcgctgg aaggaaaagg
     2701 cgttaacgca acgaggccta ctggtgcagg caggtgatca gagcgagcaa atttttaccc
     2761 gctggcgtgc tggggcgtat aacgcctggt cgttgcctgg gcgctgtttt atcgttctgg
     2821 aggagttgcg ctggggtgca tttggcgatg cctgccgtct gggaagcccg caagcggtgg
     2881 tgttgttact gggtgatttg cgcgagaaag cgacacaaca tctggcagag agtatcaatg
     2941 cggcaccgac cacgcgtcac tattaccatc agtggtttgc ctcttcggga ggggagcatg
     3001 ctgatttttt aagttggctg ggaaagtggt ccacggcaga taaacagccc gtttgctggt
     3061 cagtgaccca acgctggcaa acagtcgcgc tggggatgcc acgactctgt tcagcgcagc
     3121 gtttggtggg ggcaatggtc gaggaaatct tctctgtaaa tttggcgtaa ataatcagtt
     3181 acatcaatga gtcctaaacg aaatccatgt gtgaagttga tcacaaattt aaacactggt
     3241 agggtaaaaa ggtcattaac tgcccaattc aggcgtcaac tggtttgatt gtacattcct
     3301 taaccggagg gtgtaagcaa acccgctacg cttgttacag agattgcatc ctgcaattcc
     3361 cgctcccctt ttgcggccgt cgcgctgatt tttctggcgt ttgcggaaat gggccaactc
     3421 tgcgaggaaa gctatgctga aaaggaaaaa agtaaaaccg attacccttc gtgatgtcac
     3481 cattattgat gacggtaaac tgcgtaaagc cattaccgca gcatcactgg gtaatgcaat
     3541 ggaatggttc gattttggtg tttatggttt tgttgcttac gcattaggta aagttttttt
     3601 cccgggggct gaccccagcg tgcagatggt tgctgcactt gccactttct ccgttccctt
     3661 tctgattcga ccgcttggcg ggctcttctt tggtatgttg ggcgataaat atggtcgcca
     3721 gaagatcctc gctatcacta ttgtgattat gtcgatcagt acgttctgta ttggcttaat
     3781 accgtcctac gacacgattg gtatttgggc accgattctg ctgttgatct gtaagatggc
     3841 acaaggtttc tcggtcggcg gtgaatatac cggggcgtcg atatttgttg cggaatactc
     3901 ccctgaccgt aaacgtggct ttatgggcag ctggctggac ttcggttcta ttgccgggtt
     3961 tgtgctgggt gcgggcgtgg tggtgttaat ttcgaccatt gtcggcgaag cgaacttcct
     4021 cgactggggc tggcgtattc cgttctttat tgctctgccg ttagggatta tcgggcttta
     4081 cctgcgccat gcgctggaag aaactccggc gttccagcag catgttgata aactggaaca
     4141 gggcgaccgc gaaggtttgc aggatggccc gaaagtctcg tttaaagaga ttgccactaa
     4201 atactggcgc agcctgttga catgtattgg tctggtaatt gccaccaacg tgacttacta
     4261 catgttgctg acctatatgc cgagttattt gtcgcataac ctgcattact ccgaagacca
     4321 cggggtgctg attattatcg ccattatgat cggtatgctg tttgtccagc cggtgatggg
     4381 cttgctgagt gaccgttttg gccgtcgtcc gtttgtgcta cttggtagtg ttgccctgtt
     4441 tgtgttggcg atcccggcgt ttattctgat taacagtaac gtcatcggcc tgatttttgc
     4501 cgggttactg atgctggcgg tgatccttaa ctgctttacg ggcgttatgg cttctacctt
     4561 gccagcgatg ttcccgacgc atatccgtta cagcgcgctg gcggcggcat ttaatatttc
     4621 ggtgctggtt gccggtctga cgccaacact ggcggcctgg ctggtcgaaa gctcgcagaa
     4681 tctgatgatg cctgcctatt acctgatggt agtggcggtg attggtttaa tcaccggcgt
     4741 aaccatgaaa gagacggcaa atcgtccgtt gaaaggtgcg acaccggcgg cgtcagatat
     4801 acaggaagcg aaggaaattc tcgtcgagca ttacgataat atcgagcaga aaatcgatga
     4861 tattgaccac gagattgccg atttgcaggc gaaacgtacc cgcctggtgc agcaacatcc
     4921 gcgaattgat gaataagctg aaacggatgg cctgatgtga cgctgtctta tcaggccaat
     4981 tgaactctta aggttcactt aatctctgac gcgcatactc tcctccaggt taacggagga
     5041 gagtgcaatg aaaaaccgtg tttatgaaag tttaactacc gtgttcagtg tgctggtggt
     5101 cagcagcttt ctttatatct ggtttgctac gtactgatct ttcttcagcc gtacccaggc
     5161 ccgcgtgccg gaagtctctt gccggttttg caggaaaaac tgcccgtgat gcaactgtgt
     5221 aatgcggctg acaatactta accccagacc aatcccgcca taacggctgt ccatacgtac
     5281 aaacgcttta ctcaactccc cgcatttact ctcatcaata cccggtcctt catcttcaac
     5341 ggccatgacc gctccgccat cttcttgcag cttaatcata atgttgctgc cttgtgggct
     5401 gtaacgatgg gcgttttcta ccaggtttcg caataacatc cgcagcaggg ttgcatcacc
     5461 ctgaacggtg atgtcggcgg cgctctctgg caatagcagg gtttgctgtc gctggtcgag
     5521 catggtactg agttcgtcat acgaggggag aatgacatct tccagcagtt ttacatgttg
     5581 ataattaccg gaagaaaatg actgtccggc acgcgccagt tgcagcagct gggagacgct
     5641 ctccatcatc tgatcaagcc gtgccactaa cggtgctaca tcaatgtgat gcgttttcgc
     5701 cagcagttcc agatgcaaac gcacccccgc cagtggcgtt cgcagttcgt gcgcgacgtc
     5761 agcggtaaat aacctttcgt tatccagcgt gctcgtcagg cgactgacca gatcgttcaa
     5821 cgccgaaacc accgcttcga tttcgagggt ggcgctgtga atggcaatgg gcgttaagtt
     5881 gtcggcggtg cgcgcttcca gctctttttg cagctccgcc agcgggcggg tgatgcggcg
     5941 taccgcctga tagcagataa atagcgtcag gctgaccata aagacgccgg ggacaatcag
     6001 gctggcgacc gcctcgcgga tctcacgcat gatgtggcga tcgttgttgc gattgtcgcg
     6061 tagcgcctgc tcaaacagct gaatctgctc ggtactttca tgccatagcc agaagatgct
     6121 gatcagctca aacaccaaca aaatggcccc gatggtcaat atcagccgtt ggcgcagcga
     6181 tattggtcgg cgcagaaaac gcatcagatt caattagttt tcctcattcg cgaccagcat
     6241 atagccaaag ccgcgcacgg tgcggatacg ggctttgccc actttgtcgc gcagattgtg
     6301 gatatgcact tccagggtgt tggtcgaggg ttcattgtcc cagttataga tgtcgttgta
     6361 gagaatttcc cgatgtaccg gactgcctgc tttgagcatt aaccgtgaca gcagagcata
     6421 ttctttgggc gtcagaatca actcttcacc gcccatccat acctgacggc gacccatatt
     6481 cagcgtcaga ttgccaacaa tcagctcact ttcgccctga ttattatggc gtcgtagcag
     6541 ggcgcggata cgggcgtgta actcttccag cgcaaaaggc ttcaccagat agtcatcggc
     6601 accgacatcc agcccggcga ttttgtcggt cagcgtatcg cgagcggtga ggatcagtac
     6661 cggcagggtg tattttttct gccggatacg ggcgagaaaa tgcagtccat cttcgtcggg
     6721 taaccctaaa tccagtacca ccaggctgta atgaccggcc tcaaggcttt gttccgccat
     6781 ccgcgcggtt gttacgccat cgcacgtgta gccttcggtt tgcgccgcca gaatcagtcc
     6841 ctgcaataac agcgtatcgt cttcaacaat cagaattttc attcactcac tctcctgcaa
     6901 gtttgcagaa catcatccgc agcctggtaa tacttcgtct caacgccagt taatcccaat
     6961 agcgtggaga ataaattgtc ttgtgaatag tgttgcgttt gcgcctgttt ttgcaggcag
     7021 ttctggtcaa cctgataccg tttttgataa tcctccgaca gccacagcag catcggcacc
     7081 tgtttttggc tatccggggc gatggcataa ggcagaccgt gcagatagat gccattttca
     7141 cctaacgatt caccgtggtc agaaagataa accaggctgg tggtaaattt atcctgatgt
     7201 tctttcagca gattaatcgc tttatcaaca atatagtcga cgtaaaccag cgtgttgtcg
     7261 taagtgttca ccagttgctc tttggtacag gtctggatct cattggtgtc gcaggttggg
     7321 gtaaatttcc tgaactgcgg cggatagcgg ttgtaatagg tcggaccgtg gctgccgatg
     7381 gtgtgtaaga caatcacgcc atcaccctgc aggttattga tgtactcttc cagcccgtgg
     7441 aacagcactt cgtcatagca ttcgccgttg atgcactgac caggcagatt cagcgcggtg
     7501 acgttctggt gaggcacgcg gtcgcaggca cctttacagc cgccatcgtt gtcattccac
     7561 agcacgttga tgcccgcttg ctgaatgata tccagcacgc cttcctggtg ctgtgccagc
     7621 tcttctttgt aatgctcacg cggcatatcc gagaacatgc acggtactga aactgccgtt
     7681 gccgtgccgc aagatgcggt attagggaaa tagaccacgt tatctttcgc cagccgcggg
     7741 ttagtttcac gcgggtagcc gttgagggag aagttctccg cccgcgaggt ttcgccgaca
     7801 atcaggatgg tcaaattttt acgtttttcg ttctgcatta acgggttgcg gtgggcgtct
     7861 tcaccaattc gcaccagcgg tagatttgcc agtcgctgat gggagtacca tgaccagctg
     7921 gcaacaatgc tgttagaggg gcttaaggat ttcaccagct ctttgttatt gcggaacaac
     7981 gaggcgtagt ctttataaaa cagtgcggcg accagcaaaa tcagcagtac agaaaccaga
     8041 atattggctc cacggaaaag aacactgcgc agacgcgagg tggcaggttt gatttttagc
     8101 cagcaggcaa tcagcgcagc aagcacgccg ctgaatccca gcgttaataa catttgcggt
     8161 gtcatcagcg cataactttc tgctggagtg gtatcaataa tattggcaat catcgagcgg
     8221 tcgatgacga tgccgtaagt cattatgaaa tattgtgcag ccgcgccaac cagaataaac
     8281 aggcaggcca gtggtcgatt aagccataag aaagagctta gtgtcaggac aatattaatc
     8341 acgctgaaag cgacgaccgg catcgacaag aaaaccagta cgttatgcag cgaatccagc
     8401 ggcagcgcct gcaacacctg tttaaaaaag gcaatattca ggcagataga gatataaaaa
     8461 gcggccaaca atagccaggc gagtaaattc aaagaggggc tttttagtag gcgcttcaac
     8521 atcacggtgt ttccatcgaa caaagtgcgc atatgctcgc aaagcaaaat taagccaacc
     8581 ttaagttctt aaggttggct tttatgtttg ctggattaag gggaattaat ctttgcttat
     8641 tggtgcatct aagggatacg ggtttttatg tagccggttg taattcagcg catacatggc
     8701 ggtgatgacc atcagggtga caaatgacca cataacctct ttcgccccgg accccaccac
     8761 ggcccagatg cagtagagga aggcaatggt agtaacagcc agatatgccg ggcgtgcttt
     8821 accaaagtga ccgtggccga gcagcagtaa cgccgcacag gtgtaaagat atggcaccag
     8881 tgtaaagatg accgacacgg aagataccag accgaactct ttggttgcgt ttggcgaaat
     8941 gctactgagc tggaagatgg tcatcaaaat accgacgata atcagccccg ccactggcgt
     9001 acccgcttta tttacacggg caaaaatcgg tgggaacagt ccgtcatcgg cagcggcttt
     9061 cgccgtttga cccgccagca acgtccagcc gcccagtgaa cctaagcaac ccgcagctgc
     9121 gcagaaggaa acaatggccc cagcggtgtc acccaacgcc atccgtgcgg catcaccgaa
     9181 tggcgaggca gaaacgcgca gtgcggcatt agggatcatc cccataatcg cggtggtgga
     9241 gagcacatag caaacggcgg caatcaatac cccaccaatg gtggcgatag ggacattgcg
     9301 tttcgggttt ttcaccacac ctgcggcaac ggaggcactt tccacaccga tgaacgacca
     9361 cagcgtaacg ttaagggtac tttgaatcgc accgaaggtg cccaggccgc tgacgttcca
     9421 tgccgccata taggtttcac cacggaacca gaaccagcca aatacggcaa tcccgacgat
     9481 ggggatcagc gccagcacgg tggcaacggc ctgcacacgg gtgatcattt tcggaccgac
     9541 aatgttcagc aggacgaaga tccacagcac cacgacgcag gtgatggtta ataccaatgg
     9601 atctttcaga atcgggaaga agtaacttaa atatcctacg ccaatgacca ccatagcgat
     9661 attgccgatc cagcaggcca gccagtagag gacgttggtt tgataaccga gaaacgggcc
     9721 aaagcagcgg cgggcgtaag cgtaagaacc accaggactt ggatcgagga acgacatttt
     9781 ggcgtatacc atcgagagcc ccagcgcacc gataatcgtc accaaccatc cataaatggc
     9841 aatcccgcca gtggaggcca ggtttgcagg taacagaaaa acacctgacc ccataatatt
     9901 ccccgacacc atcagggtga cggggattaa gcccactttg tgagcatcag catccgaaga
     9961 cataattaaa ctcctgcgaa ggcgagcttc gtgacaataa attacgtcat atcatacgcc
    10021 tgcattcgat agttgaatta ttatcgaccg ggttatcgct gatgccgtta ttaatatagc
    10081 tcttcgttaa ggttaaaaat caggcgacag ttcgttctct gtgctgacta acataatgca
    10141 gcggcgtcat accgtagaag tctttaaaga cagaaataaa gtacgacgta ctgttgtagc
    10201 cgcaggactg tgatacctga gagatatttt taccgtccat cattaattca tttacggcat
    10261 aacgcatgcg gcaggtggtg attatttggc tataactggt gttttcgctt ttcagctttt
    10321 tctttaacag acttgggcta agacataaac aactggctac catactaaga ttccagtctt
    10381 tgtgaatatc gctttcaata atttgataaa cgctgtcact tacacaatta cgtaacatat
    10441 acatcattaa tgaaaggaat tttttactgt cgagaaaacg ggatagcacc gtgaataata
    10501 atgcgcgtgt tcgttccttt tctgactcag tttctgcggg catcatgctg tgttgcgcgg
    10561 caacgcgaaa gacgtctggc gtcaggcatg gcagggtgag tatgggcgta gcgctacgtt
    10621 gccatactgg tatttgcgag agatctttat tcagaaaccg gaggtaatct ttgatgatgt
    10681 cgtgactaat atgagcaacc aaagtgttgt taagagagga gacgtcgata atattatttt
    10741 cacaatttaa taacgccata tggttagctt taaggctaat aggttctttc ccattcaccc
    10801 ttatccagac atctttttca gtcaataaaa caatacaagg ttggtcgctg caaatcctca
    10861 tacatgtact cctgagtgcg aataaaagac cagttctcgc taaagcaaag cgataccggg
    10921 aaatggttat ataattgtat gatgaatata aacatgcata aaaataacga gttttactaa
    10981 tattacaaat ttttatagta atttctcatg tacatatttt tttacaggat aaataacagg
    11041 caaaaaaaag cgcggggtgg ggccgcgcaa aaagtatgac atttttgttt gcagcaattt
    11101 ttatgaaaac gttgccatac tcaaaaggcc taaggccagg cggcgtaatg ttatttaaac
    11161 aattacgcct tcagcggaat agtggttacg ctttcacaca cataacgtgg taaataccgt
    11221 caataatttc agtcccttca gtttcgtgtt caaatccagg gaaatggtgg tcccaggatt
    11281 gcagcgagcg taaataactt acttgcggac tgtttttatc gccgaagttt tcaccagaca
    11341 gcagcatcgg gattcctggc ggatatggga taactgagtt tgccgcgatg cgtcctggca
    11401 gattttcaat ggataccagt tcgacattgt tgtcgacaat cgcgttgtac gcttcacgcg
    11461 gggtgatttc cgccaccgac aggccggaat aggcttcgtt caaccgtgcg ccagggttgt
    11521 tttctttcag ccaggcaaac atggtgtcac ccagatcgtg aatccccatg ttcgcgtaag
    11581 tgtcaggata ttgttcaaca agttccggca tcacctgcgc cagcggtgtg ttggcgtcat
    11641 agtggcgttt gaaggagcaa agggtgttaa ccagagttcc ccatttccca cgggttacgc
    11701 ccatagagaa caggaacata atttggaagt cagtggtgcg ggtaggcaca atgccgtggc
    11761 gaccaagcca ggcagtgacc agcgccgccg gaacaccggt ttcttccagt tcaccatctt
    11821 cacccattcc cggagcaagg atgctgactt taatcgggtc gagcatactc cagttatccg
    11881 gaatatcttt gaagccgtgc cagctttcgc ccggatgcat tacccagcag tcctgaacgg
    11941 tggtcagcag tttggttggt gcgtcagcaa agtcataggt tttgccggtt tgtgggtcgg
    12001 tgacgacttc tttgttccac ggtttgaaga accagctacc gtcagcggtg aactctttat
    12061 acagccgcgc catcgcctga cggaaatcaa ccgcttcgtc gatcacttcc tgcgtcagtg
    12121 acaggccgct gttgccgtcc atcatcgaca ccgccacgtc gttggatgcg cagatggcat
    12181 acagcgggga ggtggtggca tgcatcatgt aggcctggtt gaagcgggag aagttaatcg
    12241 ccccacgacc ttcacgtaca tgaatataag aagcctgtga aagcgcattc agcagtttgt
    12301 gggtggagtg ggtggcgaaa acggtaggac cgttgtgatc gccaggttcg ccgcgcatgg
    12361 catagtgatc ggcatagatc gggttgaaac gtgcatagcc gtaccaggct tcgtcaaagt
    12421 gcagacgatc ggaggttttt tccagcagat cctgcgcttc tttagcgtta taacacacgc
    12481 cgtcataggt gcagttggtc accacgcagt aagacggttt ttgcccggct ttgtctttag
    12541 tcagcgggct ttcactgatt ttcttctgca aggtttcagg ttgcatttcc tgcggataga
    12601 ttggcccgat aatgccgtag cggttgcggc ttggcaccat gtagaccggt ttcgcgcctg
    12661 tcagcatcaa gccttgttcg atggatttat ggcagttacg gtcaacgacc acgacatcgt
    12721 tatcggtcat gcaagcctgc atgatggtgc ggttagagcc ggaagtaccg acgactaccg
    12781 accaggagcg atcggcacca aatacgcgtg cggcatattt ttcgctttcg ccaaatgcgc
    12841 cagtatggtc aagcaaagaa ccgagggaag ttcgttcgat gcccatatcg gtgcggaaca
    12901 gattttcacc atagtagtca tggtagaaac gtccggcggg tgttttggta aaaccaacgc
    12961 cgccctggtg gcctggcgct gcccaggaat attcatggat gtcgctatat ttcatcagcg
    13021 cgctgaacag tggcggcaac agctgctggc ggtagcgggt catcgcggca acagcgcgtc
    13081 cggcgataaa gtcggcggta tcttccagaa tccaggcgaa ttcatcgaca agctccagca
    13141 ggtcgcgatc cattgcggcg agggcttttt cccgatcgcc caacaggaag accggcacgt
    13201 tttgttggcg ctcatgaagc ttaccgatca attgtctgac gttttgatgt tcgtccggat
    13261 gttccatttg atagctgaac atcaggcagt caatggcttc gtttgaagag agaatggcaa
    13321 aaccatcatc aaaggaggtg gatttaatca cggtaacatt ttgctggctt aaagcatctg
    13381 ccagacgctc aacggcgtta ccgacccagg tgtcttgatg gagaaactcg ctttcaacaa
    13441 ttaatacttt catcattgct tacccggtta tgaaggaatc ttcgttgcgg gcaagtatct
    13501 tccggccttt tcgggctgta aagcgcgtga aaaacaaaca tttttga
//
LOCUS       DAEEKQ010000107        13247 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.107, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000107 DAEEKQ010000000
VERSION     DAEEKQ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13247
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.107"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..781
                     /gene="umuC"
                     /locus_tag="KLK57_002887"
     CDS             <1..781
                     /gene="umuC"
                     /locus_tag="KLK57_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBE0893202.1"
                     /translation="VDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLA
                     DTDIRFIRKHFNVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQ
                     AICSYAARAAEKLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDII
                     NAATRSLDAIWQAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLN
                     AKEGRGTLYFAGQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="KLK57_002888"
     CDS             complement(827..1357)
                     /gene="dsbB"
                     /locus_tag="KLK57_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HBE0893203.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     MISQPFKAKKRDLFGR"
     gene            complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="KLK57_002889"
     CDS             complement(1503..3044)
                     /gene="nhaB"
                     /locus_tag="KLK57_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HBE0893204.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAIFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     IGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            3265..3984
                     /gene="fadR"
                     /locus_tag="KLK57_002890"
     CDS             3265..3984
                     /gene="fadR"
                     /locus_tag="KLK57_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HBE0893205.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(4036..5568)
                     /locus_tag="KLK57_002891"
     CDS             complement(4036..5568)
                     /locus_tag="KLK57_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HBE0893206.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREMRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            5898..7196
                     /gene="dadA"
                     /locus_tag="KLK57_002892"
     CDS             5898..7196
                     /gene="dadA"
                     /locus_tag="KLK57_002892"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HBE0893207.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            7206..8263
                     /gene="dadX"
                     /locus_tag="KLK57_002893"
                     /pseudo
     CDS             7206..8263
                     /gene="dadX"
                     /locus_tag="KLK57_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
     gene            complement(8650..10386)
                     /locus_tag="KLK57_002894"
     CDS             complement(8650..10386)
                     /locus_tag="KLK57_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HBE0893208.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(10481..11395)
                     /gene="ldcA"
                     /locus_tag="KLK57_002895"
     CDS             complement(10481..11395)
                     /gene="ldcA"
                     /locus_tag="KLK57_002895"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HBE0893209.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIQRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAHGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCRAEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRDERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            11495..12106
                     /gene="emtA"
                     /locus_tag="KLK57_002896"
     CDS             11495..12106
                     /gene="emtA"
                     /locus_tag="KLK57_002896"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HBE0893210.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLEHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(12108..12842)
                     /locus_tag="KLK57_002897"
     CDS             complement(12108..12842)
                     /locus_tag="KLK57_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HBE0893211.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            13043..>13247
                     /locus_tag="KLK57_002898"
     CDS             13043..>13247
                     /locus_tag="KLK57_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HBE0893212.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAV"
BASE COUNT         3364 a         3367 c         3482 g         3034 t
ORIGIN      
        1 ggtggattta tcaaatctgg aacgccagcg taaattaatg tctgctctcc ccgtggatga
       61 cgtctggggg attggacggc ggatcagcaa aaaactggac gcgatgggga tcaaaaccgt
      121 tcttgatttg gcggatacag atatccggtt tatccgtaaa cattttaatg tcgtgctcga
      181 aagaacggtg cgtgaactgc gcggtgaacc ctgtttgcaa ctggaagaat ttgcaccgac
      241 aaagcaggaa attatctgtt cccgctcgtt tggtgaacgt atcacggatt atacgtcgat
      301 gcggcaggcc atttgtagtt acgctgcccg ggcggcggaa aaacttcgca gtgagcatca
      361 atattgtcgg tttatctcca catttattaa gacgtcacca tttgcgctca atgaacctta
      421 ttacggcaat agcgcgtcgg taaaactgct gacgcccact caggacagca gggatatcat
      481 taacgccgct acgcgatctc tggatgccat ctggcaagcg ggccatcgct accaaaaagc
      541 gggcgtgatg ctgggggatt tcttcagtca gggagtcgcg cagctcaatt tattcgatga
      601 caacgcaccg cgccccggga gtgagcaatt gatgacggta atggatacac tgaatgctaa
      661 agagggcaga ggaacactct attttgccgg gcaggggatc caacaacaat ggcagatgaa
      721 gcgagccatg ctttcaccac gttatacaac gcgaagttct gatttactgc gggtcaaata
      781 aatatagcgg caggaaaaaa gcgctcccgc aggagcgccg aatggattag cgaccgaaca
      841 gatcacgttt tttcgcttta aacggctggg aaatcatcac cagcaccgcg acaatcaggt
      901 aagcgataaa aataccgagc agccactgcg gcatttccag acctaaaaaa tcccactgac
      961 gctcggcgca atcgccagag gcgacaaaca cttgcggcac ccatttatcc agcggcagcc
     1021 attccgggaa acgaaccata aaatcacagg tggcaaacgg cgaaggatag agctgaagca
     1081 tggtgtgctc gtaagttaac tgcacaccgc ggaacgcact atacaaccag ataaccatcg
     1141 ctacataacg cagcggagtt ttcggggcga tcgcgccaat cagcgcagca cccagaacgc
     1201 cgaataacgc gcagcgttca taaatacaga gcacgcaagg tttcagtaac atcacatgct
     1261 ggaaccacag cgccgtcagt tccagtgcca gagcagtaaa cgccatcaac agccacgcgc
     1321 cccggccttg tgaacattgg ttcaaaaatc gcaacataat catttccctg caatatgcat
     1381 agagtgcgca gtttaaacca attcattcgc tgcgccacca ggaggcagca aaaataggac
     1441 gcaattggac aattatcggg cgaaaaggca aaccgggcat ttatatgccc ggtaagttgt
     1501 tatcaaagcg ttgctatcca gcccatttgc ataaaccatt cggttacagg ggcaagcgta
     1561 aactcgacgc agagcagtcc gacgagtgtc aggacgaggg tgtaaggcag ggccatccac
     1621 accatgcggc cataagagag gcgaatcaat ggcgcgagtg cagaggtcag caggaatagg
     1681 aacgcagcct gaccgttcgg cgtagcgaca gagggcagat tggtaccggt attaatggcg
     1741 accgccagca gctcgtattg cttcaacgtg atagcgccac tttccattgc cgctttcgct
     1801 tcgttgatat aaatcgtccc cacgaagacg ttatccgaaa tggatgacag cagaccgttg
     1861 aatatataga acagcgacag ctgagcatgt tccgatgcct gcaacacaaa ctgaataatc
     1921 ggcgaaaaca gttgttggtc gataatcacc gcgactaccg agaaaaacac cgtcaacagt
     1981 gcggtgaatg gcagagattc ggtgaaggct ttaccgatag catgctcatc ggtgacaccg
     2041 atcaatgatg tcgccagaat aatgactgac aaaccaatca aaccaacttc cgccagatgc
     2101 aacgccagcg cagtcaccaa ccagacgcca ataatcgcct ggacaatcag acggatttta
     2161 tcctgacggg tacgctggtg gcggctttga tcgtcaaact gctgcagcac ttcgcggact
     2221 ttctccggca gcgtttcacc gtagccaaac caacgcagct tctccaccag caggcaggtt
     2281 aacaggccac aaatcagaac cggaacggtc accggcgaca tgcgcaggaa gaaatcgcca
     2341 aaatgccagc cagccgcttt agcgatgatc aggttctgtg gttcgcccac catggtcatc
     2401 acgccgccta atgcggtgcc gacaccggca tgcatcatca ggctacgcag aaagccacgg
     2461 aactgttcca gaaccacttt gtaatgcttg tcgatatgac tatcgtcttg caggtcggta
     2521 tcttcagtac gggaagaggc tacgcggtga taaataccat aaaaaccgac tgcaacgctg
     2581 atcaccaccg ccacgacggt taaggcatcg aggaacgcgg agaggaacgc cgccgccacg
     2641 caaaaagaga gcgacagcag cattttggag cgaatgctta acagcaaacg ggtaaatatg
     2701 aacagcaata gctgtttcat aaaatagata cccgccacca taaacatcag taacagcaag
     2761 acttcaagat ttgccgccac ctcttcacgg acgtgttccg cgctggtcat gccgatgaat
     2821 atcgcttcga tagccaacag accaccgggg agcagcgggt agcatttcag ggccatcgcc
     2881 agagtgaaaa taaattccgc taccagcaac cagcccgcga cgaaagggct gatgaggaaa
     2941 attaacgggt ttacgattaa gaaaatgatg agggcgagtt tgtaccagtc gggggactgg
     3001 cccaaaaaat tgcgccatag cgcgcggccc caggagatct ccatgatggt ttcccttacc
     3061 ttacaaataa tcaatgatgt ttttatgttt aaacgcaaag cttaacggtc aggcaggagt
     3121 gaggcaagtc ttgatagtca aggggaaaga gatgcggaaa atgaagcctt gatccctttt
     3181 tcttcttttt gtctgctatc agcgtagtta gccctctggt atgatgagtc caactttgtt
     3241 ttgctgtgtt atggaaatct cactatggtc attaaggcgc aaagcccggc gggtttcgcg
     3301 gaagagtaca ttattgaaag tatctggaat aaccgcttcc ctcccgggac tattttgccc
     3361 gcagaacgtg aactttcaga attaattggc gtaacgcgta ctacgttacg tgaagtgtta
     3421 cagcgtctgg cacgagatgg ctggttgacc attcaacatg gcaagccgac gaaggtgaat
     3481 aatttctggg aaacttccgg tttaaatatc cttgaaacac tggcgcgact ggatcacgaa
     3541 agtgtgccgc agcttattga taatttgctg tcggtgcgta ccaatatttc cactattttt
     3601 attcgcaccg cgtttcgtca gcatcccgat aaagcgcagg aagtgctggc taccgctaat
     3661 gaagtggccg atcacgccga tgcctttgcc gagctggatt acaacatatt ccgcggcctg
     3721 gcgtttgctt ccggcaaccc gatttacggt ctgatcctta acgggatgaa agggctgtat
     3781 acgcgcattg gtcgtcacta tttcgccaat ccggaagcgc gcagtctggc gctcggcttc
     3841 taccacaaac tgtcggcgtt gtgcagtgaa ggcgcgcacg atcaggtgta cgaaacagtg
     3901 cgtcgctatg ggcatgagag tggcgagatt tggcaccgga tgcagaaaaa tctgccgggt
     3961 gatttagcca ttcaggggcg ataatccctt ccgtttaaag agcaaacccc tcaaacgggg
     4021 ggttttttgt tgtttttaca gatttcccat tcttggcggg caacgttcca gcaactcgat
     4081 gctgccgtct tcgttttgct gttcgagcat cacatcaaat ccccacaggc gatgcacatg
     4141 cttcaggact tctttgcgcc cccgatccag cggtgcgcga ttatgtggaa tataacgcag
     4201 cgtcagcgaa cggtcgccgc gcaaatccac gttccagatc tgaatattcg gctccagatt
     4261 acttaagtta tattgcgacg ataaccggtt acgcatctcc cgataacctt cttcattatg
     4321 aatagcggaa atctccagat aattatgccg atcatcatcc agcacggtga agaagcggaa
     4381 atcacgcatc actttcggtg acagaaactg gctgataaaa ctttcatctt tgaaatcacg
     4441 catcgcgaaa tgcagcgttt ccagccagtc ggaaccggcg atatctggga accagtattt
     4501 gtcttcttcc gttggcgact gacaaatccg tttaatatcc tggaacatgg cgaacccgag
     4561 ggcatacggg ttgatgccgc tgtaccacgg gctgttatag gggggctgga agaccacatt
     4621 ggtgtggctg tgcaaaaact ccagcataaa acgttccgtt actttccctt catcatacag
     4681 atggttaagg atggtgtagt gccagaaggt cgcccagcct tcgttcatca cctgagtctg
     4741 tttttgcgga taaaaatact ggctcacctt acgcacaata cgcaggattt cacgctgcca
     4801 tgattccagc agtggcgcat ttttctccat aaaatagagc aggttttctt gtggttcgga
     4861 cggatagcgg cgcgcttcag caaccgtttt ctcttcctcg cgcttcggca gagtacgcca
     4921 gagcatattg acctgacttt gcagatactc ttcgcgactt ttctgccggg ctttctcttc
     4981 ttgcagcgag attttttgcg ggcgtttgta ccggtccacg ccgtagttca tcagcgcgtg
     5041 gcacgagtcc agaagccgtt ctacttcatc cacgccataa cgttcttcgc actcggtaat
     5101 atatttacgg gcaaaaatca gataatcgac aatcgaactg gcgtcggtcc aactgcggaa
     5161 taagtaattg tttttaaaga aagagttatg cccatagcag gcgtgtgcca tcaccagcgc
     5221 ttgcatggta atggtgttct cttccatcag gtaagcgata cacgggttag agttaatgac
     5281 gatttcatag gccagccctt gctgaccgtg cttatacagc cgttcagtct cgataaattt
     5341 tttaccgaat gaccagtgcg gatagttaat tggcatgccg acgctggagt aggcatccat
     5401 catctgttct gaggttatca cttcaatctg gtgcgggtag gtatccagcc ggtagagttt
     5461 cgccacccgg tctatctctg ccagataaac atccagcagg tcgaacgtcc agtcgggtcc
     5521 atcgctcaaa cgtgtggtgt ccttattcat agaatcgatc gtcgccatac gcgcacctca
     5581 ttgttgtcgg cgctctctgt gtggagcacc tcatttcaag catagaacac ctgttaaaaa
     5641 ccgcgtcgcc ggagaatttt tttctttgcg atttcttatt atcagagtgc cactaatccg
     5701 cttctgaacg gaattttatg ctggataaaa agggcgttca gcaggagata ctaaagacgc
     5761 catattgccg cagagtcagg gagatgtgag ccagctcacc ataaaaaagc cgtatgttga
     5821 ataatgtttt caactgagtt atcaagatgt aattagatta ttattctttt actgtatcta
     5881 ccgttatcgg agtggctatg cgagttgtca tactgggaag tggtgtggta ggcgttgcca
     5941 gcgcctggta cttaaatcag gcaggacatg aggtcaccgt cattgatcgg gagccggggg
     6001 ccgccctgga aaccagcgcc gcgaatgccg ggcaaatctc ccccggatat gctgcaccgt
     6061 gggcggcacc aggtgtgcct ttaaaagcga tcaaatggat gttccagcgc catgcgccgc
     6121 tggcggttcg tctcgacggt acgcagtttc agttgaaatg gatgtggcaa atgttacgta
     6181 actgcgacac cagccactac atggaaaaca aagggcggat ggtgcgtctg gcggaataca
     6241 gccgtgattg cctgaaagca ttgcgcgccg aaaccaatat tcagtatgaa gggcgtcagg
     6301 gtgggacgct gcaactgttc cgtaccgaac aacagtatga aaatgcgacc cgcgatatcg
     6361 ccgtgctgga agatgccggc gtaccgtatc agctgctgga atccagccgc ctggcggaag
     6421 tggagcccgc gctggcagaa gtagcgcaca aactgacggg tggtctgcaa ctgcccaatg
     6481 atgaaaccgg agactgccag ctgtttaccc agaatctggc gcggatggca gagcaggcgg
     6541 gggttaaatt ccgctttaat acgcccgttg accaactgct ttgcgacggc gagcaaatct
     6601 acggcgtgaa gtgtggcgat gaagtgatta aggccgatgc gtatgtgatg gcgtttggtt
     6661 cttactcgac ggcgatgctc aaaggcattg ttgatattcc ggtgtatccg ctgaaaggct
     6721 actcgctgac tattccaatt gcgcaggaag atggtgcgcc agtatccacc attcttgatg
     6781 aaacctacaa aatcgccatt acccgtttcg ataaccgcat tcgtgttggc ggaatggcgg
     6841 aaattgttgg ttttaacacc gagctgttgc aaccgcgtcg tgaaacgctg gaaatggtgg
     6901 ttcgcgatct ctatccacgc ggcggtcatg tcgagcaggc gactttctgg acggggttgc
     6961 gcccgatgac gccagacggt acgccggttg tcgggcgtac acgctttaaa aatctgtggc
     7021 tgaataccgg tcacggcacg ctcggctgga cgatggcttg cggttccggt cagttgttaa
     7081 gcgatctgct ctctggtcgc acgccagcga tcccatatga ggatctaagc gtagcgcgct
     7141 acagccgtgg atttacgcca tcacgtccgg gccatttaca tggcgcacac agctaaggaa
     7201 acgagatgac ccgtccgata caggccagcc tcgatctgca ggcattaaaa cagaatctgt
     7261 ccattgtccg ccaggccgcg ccgcacgcgc gcgtctggtc ggtggtaaaa gcgaacgctt
     7321 acgggcatgg tattgagcgt atctggagcg cgctcggggc caccgatggc tttgcattac
     7381 ttaacctgga agaggcaata acgttacgtg agcgcggctg gaaggggccg atcctgatgc
     7441 tggaaggatt tttccatgct caggatctgg agatttatga ccagcaccgc ctgaccacct
     7501 gcgtacacag caactggcag ctcaaagcac tgcaaaatgc gcggctaaaa gcaccgttgg
     7561 atatttatct taaagtgaac agtgggatga atcggttggg cttccagccc gatcgcgtgc
     7621 ttaccgtctg gcagcagttg cgggcaatgg cgaatgttgg cgagatgacc ctgatgtcgc
     7681 attttgccga agcggaacat cctgatggaa tttccagcgc gatggcgcgt attgagcagg
     7741 cggcggaagg gctggagtgt cggcgttcgt tgtccaattc ggcggcgact ctgtggcacc
     7801 cggaagcgca ttttgactgg gttcggcctg gcattatttt gtatggcgct tcgccgtccg
     7861 gtcagtggcg tgatatcgcc aataccggat tacgtccggt gatgacgcta agcagtgaga
     7921 ttattggtgt ccagacgcta aaagcgggtg agcgtgtggg ctacggcggt cgctatactg
     7981 cgcgcgatga acagcgaatc ggcattgtcg ccgcagggta cgccgacggt tatccgcgcc
     8041 acgcgcctac cggtacccct gttttagtgg acggcgtgcg caccatgacg gtggggaccg
     8101 tctcgatgga tatgctagcg gtcgatttaa cgccttgccc gcaggcgggt attggttgtg
     8161 gggcaaggag atcaaaattg atgatgtcgc cgccgctgcc ggaacggtgg gctatgagtt
     8221 gatgtgcgcg ctggtgctac gcgtcccggt tgtgacggtg taacttgttg taagccggat
     8281 cggaggcaac gtcttctggg tgcaaaaaaa atcatccatc cggctggtca gcaactgtag
     8341 ttgttaatgt gacagagcca ttgcccatga tagtgtccat taaaaggatg gacactattt
     8401 ccccggaacc tgaactcacc gcacaggcgt tctacataaa acgcttacgc ttcattgttg
     8461 actcgaactc gacttcagat aaatcacgct ttacccttga tggagcctgt acatagattt
     8521 gtgtaattgc ctgattttga tatgttcaat tcaacatcaa atgaaggtta aattatggac
     8581 gacaaacaat tgcaggctca ggctgcgttc agcaaagcat cgcaaccggc gatagatgct
     8641 tcattaaatt taagattcag cttcctcttc agccacccgt acgccaatct tcaacacttc
     8701 attatcttct ttctcggcca ccgtccagat catcccggca aactctacct ggtcaccgac
     8761 aaccggtgcc gcgcctaaca actgctggac aatttcaccc agcgtttgct gcttatcacg
     8821 atattctcgc ccgtcttcca ggccatatat cagcgcaaca tcagcatatt tggcgctggc
     8881 ttcgagaatg aagtcaccaa agaagcgttg atccagcgcg accggtggcg actggctgaa
     8941 cagtttaccg agcgccggga gatcgcgttc ccgaccaatt acacacaaca catcgccttc
     9001 gcgcagtcgg gtgctgccgg taggatgaag caactggtta tcacgaaata gtgccgcaat
     9061 acgcgtctct tttggcatat gcagatcgcg cagtgccgca cctacacacc atttatcggc
     9121 actcaattga taaacaaact gctcccacgg attttccgga tgaatatcca gaccaacgcg
     9181 tgacactgga cgtccgacgg gcggaaccac cactttggct tttttcgccg cccacgagag
     9241 tgatgttccc tgcaagagca gtgaaaccag aaccacaaag aaggcgacat taaagaacag
     9301 acgtgcattc tccagccccg ccatcatcgg gaacacggcc aggatgattg gcaccgcgcc
     9361 gcgtaatcct acccagctga taaacacgcg ctcacgcaga ttgaaaccgc ggaaggggag
     9421 caatccagca aataccgaaa ggggacgggc gaagaatatc atccatgccg acaaaatgag
     9481 cgccggaatg gcaataggca gcagatcgct tgggttaacc aatagcccca gcaccaggaa
     9541 catggcgatt tgcgccagcc aggcgaggcc gtcgaaattt tgcaggatgc cgtagcggtt
     9601 gcgaatcggc cgattaccca gcagaaaacc gcacagataa acagccagaa taccgctgcc
     9661 ttccagcgcc gtggttaaag caaaaattaa aataccgccg cttaatgcca gcaatggata
     9721 taatccggca ggcagggcga tgcgattaat catttgcagc agtaaataac caccgccaag
     9781 tccgatgaca atcccgaggc caaattgctg cagaatatcg accacgaaca tccaactgac
     9841 actgctttca tgttgctgga tcatcgcaat cagggtaatc gtcagaaaga ccgccattgg
     9901 atcattactg ccggattcaa tttccagcgt cgagccaaca cgttcgttaa gccccttacc
     9961 acccagcaga gaaaagaccg ctgcagcatc ggtagagccg acgatagcgc cgattaataa
    10021 gccttcaatc aaatcaagat taaacagcca cgccgccatc atgccggtta aaccagaggt
    10081 gataagcacg cccagcgtcg ccagcgacag tgccggtcct aacgccacac gaaaggaact
    10141 ggcctgagtg cgcatcccgc cgtcgagcaa aataatcgcc agtgccaggt tacttaccat
    10201 gtaggcgaag gggtaattat caaacgggat accgccgacg ccatcgactc ctgccagcat
    10261 gccgatcgcc agaaaaataa ccagaatagg aatgccaaga cgggaagaaa atgaactaag
    10321 taatatactg ctggtgacaa ggatggatcc taagatgaaa aggctaatta ttgttgtggc
    10381 atccaacggt cggttactcc tgattacgct gtctcttata taaaccctac catattagcg
    10441 gcagagacag cgttttactt agccctgaag cgtgattttt ttacatttta agaacaggat
    10501 gaccgctaat cgtcaactga gtgccttccc gggtgttatt cagaatggca tgtgcgccca
    10561 acggcagcgt aaccgtgcgt tgttcatgac caaaatcgag gccggtaatg agtggaatcg
    10621 acaggcggga acgcaaaaac gcgtacactg actccaggtt gtaacctgcg tcataatcat
    10681 tgggcgtgct gccgctaaag ctaccgagaa tgatcgcctt ctgacgtggc aaaattcccg
    10741 catgatagag ctgcaacagc atacgttcgt cccggaaagg gtgctcgtta atatcttcca
    10801 gcaccagaat accgttctca atttttggca tccacggtgt accgatcagt gaaatcagca
    10861 tcgcaagatt gcctccccac aacgtgcctt cggcccgaca tgtcgggcct tccccttgcc
    10921 attcaatggt gaaggtttca ttgcgtaacg ccagccagaa atggtgctcg gtaaaggcgt
    10981 tcagttcatc cgcgccaaag tttgctacca gcatggggcc gctaaaggtg atgacattgc
    11041 catgcgccag aagaccgcac tgaatggcgg taaaatcgct atgcccgcaa atgagcaacg
    11101 ggtcatgttg ttggcgagcc accagtgcct gccagtcaat atctgccagt aaacgactgg
    11161 caccgtaacc gccgcgtaca gccagcacga tggtgttggg ggtggtcagt ctggcaaggg
    11221 aattgagatc ttccaggcgc tccgtttccg tacccgcaaa acgctcacaa cgacgggcaa
    11281 taacctcgac gttattgacc tgatgcccct cgtcggtaag gcgctggata ccacgcagcg
    11341 cggcgtgctg tttaatgcag taacccgatg gggcaattaa gtgaaacaga gacatggcaa
    11401 ttccttgctg acaacagaaa cgaaatgtat atcatgccgc ttaggtgtgc cgttgtcacc
    11461 tcaacggcga ttccaggcta taaggataga agaagtgaaa ttgagatggt ttgccttttt
    11521 gattgtgtta ttagcgggtt gttcatcaaa gcatgactat acgaacccgc cgtggaacgc
    11581 gaaagttccg gtgcaacgtg cgatgcagtg gatgccaata agccagaaag ccggtgcagc
    11641 ctggggcgtc gatccacaat tgatcacggc gattatcgct atcgaatcgg gtggtaatcc
    11701 gaatgcggtg agtaaatcga atgccattgg tttgatgcag ttaaaagctt caacctccgg
    11761 acgtgatgtt tatcgccgta tgggctggag tggtgagccg acgaccagcg agctgaaaaa
    11821 tccggagcgt aatatttcaa tgggggcggc ttacctgaat attctggaaa ccggcccgct
    11881 ggcaggcatt gaagatccga aggtactgca atatgcgctg gtggtgtcat acgctaacgg
    11941 ggcaggtgcg ctgctacgga ctttctcgtc agatcggaaa aaggcgatca gcaaaatcaa
    12001 cgatttggat gctgacgagt tcctcgaaca cgtagcgcga aatcaccctg cgccgcaggc
    12061 tccgcgctat atctacaaac ttgagcaggc actggacgcg atgtaaatca gtcgcgcact
    12121 ttgtccgctt tttcccgggc ttctcgctcg agagagaaaa taatccgctg taattgccgc
    12181 tccaccgtcg ggctgacgtt aagaaaacgg aagctcagac ggggagtggt gatggtttca
    12241 ttcttgccat caatcacttt gcgctcgctg atagagatta actgggcgtc aaagtgaaaa
    12301 acaccccatt gccccatgtt gacttcaatc tgagcgaagc gcatgccttc ttgtaattcg
    12361 gcaggctttg ctgtttccag taatgcgccc atgccgccta acgacaaatc atacaggcgg
    12421 aaacgtaacg tactgttatc cgccagtttg gtctggcaaa aataaggcgg atggagtggg
    12481 gcggagatgc ggaaatatcg gcgtcgttgt acaaaccata aggtgggagg cggtacggtg
    12541 ataaatgccg gaagctgcaa gtattcactc tgctgtagtt gttcaacagt aaactcgact
    12601 ttcgcaccct gagtttcggc ggtaatggta atgtgctgtg cctttagcac agcgatgttg
    12661 tcttcggctt gactgccgaa atccagcacc agtttatccg gggttattgc cagtattttg
    12721 ctgatcagct gcccgccatt ccaactgaga cgcaaaggaa ttgcggcttt gtgcaaatcg
    12781 cgtaacacgc ccaggacggc taacggattt tgtttcagga actgctcatg gtaatgactc
    12841 acgcggaaaa actcctgact gacaaactgt ctttggttta tcggtcacag ttaacaaaac
    12901 ttaatacaaa tgcgtgaata ttttttacat gttgttctta aatcagccga catacgccca
    12961 gcatcgatcc cgcccttgcc tatacttaga gcgttgacgt aagcatttct tgcgtccgat
    13021 tcatcgaaac gagggcctga acatgggaat tattgcttgg attatttttg gcctgatagc
    13081 cggcattatc gccaagctaa tcatgccggg gcgtgatggt ggtggatttt tcctgacctg
    13141 tattctcggg atagtcggtg cggtggtcgg cggctggctg gcgaccatgt ttggcattgg
    13201 cggctccatc agcggtttta atttgcacag cttcctggtg gcggtgg
//
LOCUS       DAEEKQ010000108        13156 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.108, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000108 DAEEKQ010000000
VERSION     DAEEKQ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13156
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.108"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..1225
                     /gene="narU"
                     /locus_tag="KLK57_002899"
     CDS             <1..1225
                     /gene="narU"
                     /locus_tag="KLK57_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HBE0893213.1"
                     /translation="VTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGG
                     RRWTVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFP
                     KAKQGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAW
                     IWVPLLAIATIAAWSGMNDIASSRASIDDQLPVLQRLHLWLLSLLYLATFGSFIGFSA
                     GFAMLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSA
                     LLFLTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSD
                     EQAQKEAITETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCV
                     LLTWLVYGRRKFSQK"
     gene            1307..5047
                     /gene="narZ"
                     /locus_tag="KLK57_002900"
     CDS             1307..5047
                     /gene="narZ"
                     /locus_tag="KLK57_002900"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HBE0893214.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDQPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKIYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGSI
                     GFRWGEKGKWNLESIAAGKETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIETIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5044..6588
                     /gene="narH"
                     /locus_tag="KLK57_002901"
     CDS             5044..6588
                     /gene="narH"
                     /locus_tag="KLK57_002901"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HBE0893215.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFKNTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            6588..7283
                     /gene="narW"
                     /locus_tag="KLK57_002902"
     CDS             6588..7283
                     /gene="narW"
                     /locus_tag="KLK57_002902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HBE0893216.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNQYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7280..7960
                     /gene="narI"
                     /locus_tag="KLK57_002903"
     CDS             7280..7960
                     /gene="narI"
                     /locus_tag="KLK57_002903"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HBE0893217.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRSLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8039..8932
                     /locus_tag="KLK57_002904"
     CDS             8039..8932
                     /locus_tag="KLK57_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HBE0893218.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHSDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFELPLEGEVRAAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLNALTAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGNVLCVKGHQGRALGRDGMIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9028..9873)
                     /gene="nhoA"
                     /locus_tag="KLK57_002905"
     CDS             complement(9028..9873)
                     /gene="nhoA"
                     /locus_tag="KLK57_002905"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HBE0893219.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELGFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEGEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQPMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVASLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10046..10615
                     /locus_tag="KLK57_002906"
     CDS             10046..10615
                     /locus_tag="KLK57_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HBE0893220.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVLITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSVREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(10619..10846)
                     /gene="pptA"
                     /locus_tag="KLK57_002907"
     CDS             complement(10619..10846)
                     /gene="pptA"
                     /locus_tag="KLK57_002907"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HBE0893221.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIEPQMEALIKKPGYSM"
     gene            complement(10946..12081)
                     /locus_tag="KLK57_002908"
                     /pseudo
     CDS             complement(10946..12081)
                     /locus_tag="KLK57_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(12221..>13156)
                     /locus_tag="KLK57_002909"
     CDS             complement(12221..>13156)
                     /locus_tag="KLK57_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBE0893222.1"
                     /translation="IPVHDTIARVVSCISPAKFHECFINWMRDCHSTDDKDVIAIDGK
                     TLRHSYDKSRRRGAIHVISAFSTMHSLVIGQIKTDEKSNEITAIPELLNMLDIKGKII
                     TTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEKKFPLTELNNPAHDSSAMSE
                     KSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMMVRYYI
                     SSADLTAEKFATAIRNHWHVENNLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINV
                     LTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
BASE COUNT         3128 a         3367 c         3335 g         3326 t
ORIGIN      
        1 agttaccgtt aatctcaata aaatcggttt taattttact accgatcaac tctttttatt
       61 aaccgcatta ccctccgttt ctggcgcatt attgcgtgtt ccctactcct ttatggtgcc
      121 tatattcggt ggacgccgat ggacggtttt tagtactgca atcctgatta ttccttgcgt
      181 ctggctcgga attgccgtgc aaaatccgaa tactcctttt gggatattta tcgttatcgc
      241 tttgctatgc ggttttgcag gtgcaaactt tgcttcgagc atgggcaata tcagtttctt
      301 ctttccaaaa gccaaacaag ggagcgctct tgggattaat ggcggattag gaaacttagg
      361 tgtaagtgta atgcagctgg ttgcaccgct ggtcattttt gtacctgtat ttgcctttct
      421 cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg tcgctggcga atgccgcatg
      481 gatttgggta ccgctactgg cgattgccac gatcgccgcc tggtcaggga tgaatgatat
      541 cgccagttca cgcgcctcaa ttgacgacca gctccctgtc ttacaacgcc tgcatctctg
      601 gctgctgagc ctgctttacc ttgccacctt cggttcgttt atcggttttt ctgcgggttt
      661 tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt ctgcgcctgg cgttctttgg
      721 cccatttatc ggtgccatcg cgcggtcggt tggtggtgct atttccgata aattcggcgg
      781 cgtgcgggtg acgttgatca actttatttt tatggcgatt ttcagtgccc tgctgttcct
      841 taccttaccg ggcacaggct ccggtaattt catcgccttt tacgccgtat ttatggggct
      901 gtttctgact gcgggtctgg gaagtggttc tactttccag atgatcgccg tcatctttcg
      961 ccagataacc atttatcggg tgaagatgaa aggcggtagt gatgagcaag ctcagaaaga
     1021 agccatcacc gaaacggcgg cggctctggg ctttatctca gccattggcg cagtgggcgg
     1081 cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg accggctctc cggtcggcgc
     1141 gatgaaagtg tttttaatct tctacatcgt ttgtgtgctg ctgacctggc tggtttatgg
     1201 tcggcggaag ttcagccaaa aataagagat cgatgcctga tgtacaaccg tgtcgcatcc
     1261 gacaaggcat cagatcccaa ttcattcctg gagcaggagt catgtcatga gtaaactttt
     1321 ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt gccgatggtc acggacaggt
     1381 gatgcatagc aaccgcgact gggaggacag ctatcgccag cgttggcagt tcgacaaaat
     1441 tgtgcgttcc acccacggtg ttaactgtac aggctcctgt agctggaaaa tctacgttaa
     1501 aaatggtctg gtgacctggg aaatccaaca gaccgactac ccgcgcactc gccctgacca
     1561 gcccaatcat gaacctcgcg gctgcccgcg tggcgcaagt tactcctggt atctctacag
     1621 cgctaaccgc ctgaaatacc cgctcattcg taaacgactg attgaactgt ggcgtgaagc
     1681 tctcaagcaa cacagcgatc cggtactggc gtgggcatcg attatgaacg atccgcaaaa
     1741 gtgcttaagc tacaaacaag tgcgtgggcg cggcgggttt atccgctcca actggcagga
     1801 acttaaccaa ctgattgccg ccgctaacgt ctggaccatc aaaatctatg gtccggatcg
     1861 cgttgccggt ttctcgccga tcccggcgat gtcgatggtg tcatacgctg ccggaacgcg
     1921 ttatctgtcg ctgttaggcg gcacctgttt aagcttctac gactggtatt gcgacctgcc
     1981 gcccgcctcg ccgatgacct ggggcgaaca aaccgacgtg ccggaatctg ccgactggta
     2041 taactccagt tacatcatcg cctgggggtc taacgtaccg cagacacgta cgccggacgc
     2101 ccacttcttt accgaagtac gttacaaagg caccaaaact atcgccatta cccctgacta
     2161 ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg aaacaaggca ctgatagcgc
     2221 cctggcgatg gcaatgggcc atgtgatttt aaaagagttt catctcgata atcccagcga
     2281 ctactttatc aactactgcc gccgctatag cgacatgccg atgctggtaa tgctggaacc
     2341 tcgcgacgat ggtagctacg tacccgggcg catgatccgc gcgtctgacc tggtagatgg
     2401 actaggcgaa agcaataatc cgcagtggaa aaccgtggcg gttaataccg caggtgaatt
     2461 ggtagtgccg aatggttcga ttggtttccg ctggggagaa aaaggcaaat ggaatctgga
     2521 atccattgcc gccggtaagg aaaccgaatt gtcgttaacc ctgctcggtc aacatgacgc
     2581 tgttgcaggc gtggccttcc cctactttgg cggcattgaa aatccgcatt ttcgcagcgt
     2641 aaaacacaat ccggtgctgg tgcgccaatt gcccgttaaa aacctgacgt tagtcgatgg
     2701 caacacctgt ccggtggtca gcgtttatga tttggtgctg gcgaattacg gcctcgatcg
     2761 cgggctggaa gatgaaaaca gcgcgaaaga ttacgctgaa atcaaaccgt acaccccagc
     2821 ctggggtgag caaattaccg gcgtaccgcg ccagtatatt gaaaccatcg cccgcgaatt
     2881 tgccgatacc gcccataaaa cgcatggacg ctcaatgatt atcctcggcg ctggcgtcaa
     2941 ccactggtat cacatggata tgaactaccg tgggatgatc aatatgctga tcttctgcgg
     3001 ttgtgtcggg caaagcggcg gcggctgggc gcactatgtc ggtcaggaaa aactgcgccc
     3061 acaaaccggc tggttgccgc tggcctttgc gctcgactgg aaccgcccac cgcgccagat
     3121 gaacagtacc tcgtttttct acaatcattc cagccaatgg cgctatgaaa aagtctctgc
     3181 tcaggagtta ctttcaccgc ttgccgatgc cagtaagtac agcggtcatc tgattgattt
     3241 caacgttcgc gccgaacgca tgggctggct accttctgcg ccgcagctgg ggcgtaaccc
     3301 gctcgggatt aaagctgaag ccgacaaggc cggattatcc cccacagaat ttaccgccca
     3361 ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa ccagatagcg gcagcaatca
     3421 tccgcgtaat ttgtttgtct ggcgctctaa cctgcttggc tcctccggca aaggccacga
     3481 gtatatgcag aagtatctgc tggggaccga aagcgggatt cagggcgagg aactcggtgc
     3541 cagcgacggg atcaaaccgg aagaagtcga gtggcaaact gcggcgattg aaggcaagct
     3601 cgacctgctg gtgacgctcg acttccgcat gtccagtacc tgcctgttct ccgatatcgt
     3661 tctgcccacc gccacctggt acgaaaaaga cgatatgaac acctcggata tgcatccgtt
     3721 tattcatccg ctttctgcgg cggtcgatcc ggcgtgggaa tcacgcagcg actgggaaat
     3781 ctacaaaggt atcgccaaag cattttcgca agtgtgcgtg ggtcatcttg gcaaagaaac
     3841 cgacgtggta ttacaacccc tactgcatga ctctccggca gagctctcac agccgtgtga
     3901 agtgctcgac tggcgcaaag gcgaatgcga tctgattcca ggcaaaaccg cgccgaatat
     3961 tgtggcggtg gagcgcgact accctgctac gtatgaacgc tttacctcgc tcgggccatt
     4021 gatggacaaa cttggcaatg gtggtaaagg gatttcgtgg aatacccagg atgaaatcga
     4081 tttcctcggc aaactcaatt acaccaagcg tgatggcccg gcacaggggc gtccgctgat
     4141 tgacaccgcc attgacgctt cagaagtgat tctggcactg gcaccggaaa ccaacggtca
     4201 tgttgcagtc aaagcgtggc aggcgctggg cgagatcacc gggcgcgaac atacccatct
     4261 ggcgctgcac aaagaggacg agaagattcg ctttcgcgat attcaggcgc agccgcgtaa
     4321 aattatctcc agccccacat ggtccggtct ggaaagcgat cacgtctcct ataatgcggg
     4381 atacaccaac gttcatgagt taattccgtg gcgcacgctg tcgggacgcc agcagctcta
     4441 tcaggatcat ccgtggatgc gtgcttttgg tgaaagcctg gtggcttatc gcccgcctat
     4501 cgacacccgt agcgtcagtg agatgcgcca gatcccgcca aacggcttcc cggaaaaagc
     4561 acttaacttc ctgacgccgc accagaaatg gggcattcac tcaacctaca gtgaaaacct
     4621 gctaatgctg acgctctctc gcggtggacc gattgtctgg atcagcgaaa ccgatgcccg
     4681 agaactgacc attgtcgata acgactgggt ggaagtcttt aacgccaacg gcgcgctgac
     4741 ggctcgtgcg gtggtcagcc aacgtgtacc gccgggtatg accatgatgt atcacgccca
     4801 ggaacgcatt atgaatattc ctggttcgga agtaactggc atgcgcggcg gcattcataa
     4861 ctcggtcacc cgtgtttgcc cgaaaccgac gcatatgatt ggcggttacg cgcagctggc
     4921 ctggggcttt aactactacg gcaccgtcgg ctcgaaccgc gacgaattca tcatgatccg
     4981 caagatgaag aacgttaact ggctggatga tgaaggtcgc gatcaggtac aggaggcgaa
     5041 aaaatgaaaa tacgttcgca agtcggcatg gtgcttaacc tcgacaaatg tatcggttgc
     5101 catacctgtt cggtgacctg taaaaacgtc tggaccggac gcgaaggcat ggagtacgca
     5161 tggtttaaca acgtcgaaac caaaccgggc attggttatc cgaaaaactg ggaagatcag
     5221 gaagagtggc aaggcggctg ggtccgcgat gtgaatggca agatacgccc gcgtctgggc
     5281 agcaagatgg gcgtaataac caaaatcttc gccaacccgg tggtgccgca gattgatgat
     5341 tactacgaac ctttcacctt cgactacgaa catttgcata gcgcaccgga aggcaaacat
     5401 atccctactg ctcgcccgcg ttcgctgatt gacggtaagc ggatggacaa agtgatctgg
     5461 gggccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg cgaccgcaac
     5521 ttcgaggcca tgcaaaagga gatgtacggg cagtttaaaa acaccttcat gatgtacctg
     5581 ccgcgcctgt gcgaacactg cctcaatccc agttgcgtgg cgacctgccc aagcggcgct
     5641 atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg ccgtggctgg
     5701 cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa aagcggcaag
     5761 tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac cgtgtgctca
     5821 gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc cgaccgcatt
     5881 gaggaagcgg cgagcaccga gcgcgaagtt gacctctatg aacgtcagtg cgaagtgttc
     5941 ctcgatccac acgatccctc agtgatcgag gaagccctga aacaaggtat tccacaaaac
     6001 gtgattgaag ctgcccagcg ttcgcctgtc tacaaaatgg cgatggactg gaaactggcg
     6061 ctaccgctgc accctgaata tcgcaccctg ccaatggtct ggtacgttcc tccgctgtcg
     6121 ccgattcagt cctacgcaga tgcgggcggt ttgccgaaaa gcgaaggcgt gctgcccgcc
     6181 atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata tgttgagtgc cggcgatacc
     6241 ggtccggtac tgcgggcgct gaaacggatg atggcgatgc gccactatat gcgttcacaa
     6301 accgtggaag gcgttactga tactcgtgcc atcgacgaag taggcctgag cgtcgcccag
     6361 gtcgaagaaa tgtatcgcta tctcgccatt gccaactatg aagatcgttt tgttatcccg
     6421 accagccatc gggaaatggc gggcgatgcc ttcgcagaac gcaacggctg cggttttacc
     6481 tttggcgacg gttgccacgg ttcggacagt aaattcaacc tgttcaacag tagccgtatc
     6541 gatgccatca acatcaccga agtgcgcgac aaagcggagg gcgaataatg cagatcctca
     6601 aagtgatcgg cctgttgatg gagtatccgg atgagctgtt gtgggaatgc aaggaggacg
     6661 cgctggcgtt gatccgccgc gacgcgccga tgcttacgga tttcacccac aacctgctta
     6721 acgcgccgct gctggataaa caggccgaat ggtgcgaagt gtttgaccgc gggcgcacca
     6781 cgtcgctgct gctgttcgaa catgttcatg ccgagtcccg cgatcgcggc caggcaatgg
     6841 tggacctgct ggcggagtat gaaaaggtcg gcctgcagct ggattgtcgg gaactgcccg
     6901 attatctacc gctgtatctg gagtatttaa gcgtactgcc tgacgatcag gcgaaagaag
     6961 gattgctcaa tgttgcgccg atcctcgccc tgcttggcgg tcgcttaaaa caacgcgagg
     7021 caccgtggta cgcgttgttt gatgctctgc tgcaactggc gggaagtata ctttcaagtg
     7081 acagtgtcac aaaacaagta aacagcgaag agcgcgatga cacccgccag gcgcttgatg
     7141 cagtgtggga agaggaacag gtgaagttta ttgaagataa cgccacggcg tgtgacagct
     7201 cgccgcttaa tcaatatcag cgacgcttta gccaggatgt cgcgccgcag tatgtcgaca
     7261 tcagtgcggg aggtgggaaa tgattcagta tctgaacgtc tttttttacg atatctaccc
     7321 gtacatttgt gcgacggtgt ttttcctcgg cagctggctg cgctacgact acgggcagta
     7381 cacctggcgc gcctcctcaa gtcaaatgct cgataaacgc gggatggtga tatggtcgaa
     7441 tctgttccat atcggcattt tggggatttt cttcgggcac ttgttcggca tgttaacgcc
     7501 gcactggatg tacgcgtggt ttttgccagt ggcagcgaaa caactcatgg cgatggtgct
     7561 cggcggtatt tgcggtgtgt tgacgttgat tggcggcgca ggactgctgt ggcgcagtct
     7621 gacaaatcag cgcgtgcgtg ccacttccac tacgccggat atcatcatca tgagcatttt
     7681 gctgatccag tgtctgttgg ggctaagtac cataccgttt tcagcacagt atcctgatgg
     7741 tagcgaaatg atgaagctgg tgggttgggc gcaaagcatt gtcactttcc gtggtggatc
     7801 gtcagagatg ctcaacggcg tagcgttcgt cttccgcctg catctggtgt tgggaatgac
     7861 catcttcctg ctcttcccgt tcacccgact ggtgcatgtg tggagcgcgc cgtttgagta
     7921 ctttactcgt cgatatcaaa ttgtgcgttc gcggcgataa tttcaccctg gccggatagc
     7981 ccttcgctct ccggccactt gcaggctatg atagttctcc agtcacagga gaacgttaat
     8041 gaaaccgcag gtgtaccacg tcgatgcttt tacctctcaa ccatttcgcg gcaattctgc
     8101 cggtgttgtt ttccccgccg ataatcttag cgaagcacaa atgcagctta tcgcccgcga
     8161 gttaggccat tcggaaactg cttttctgct gcacagcgac gacagcgatg tgcgcatccg
     8221 ctactttacg ccaacggttg aagtgccgat ttgcggtcac gcgacagtag ctgcgcacta
     8281 tgtacgtgcg aaggtgttag gtttaggaaa ttgcacgatc tggcaaacat cgctggcagg
     8341 aaaacatcgc gtgactatcg aaaagcacaa cgatgattat cgtatttcgc tggaacaagg
     8401 tacgccgggc tttgaactgc cgctggaggg tgaagtacgc gcggcgatta tcaacgcatt
     8461 gcatcttact gaagacgata ttctgccggg tttgccgatc caggtcgcga cgaccgggca
     8521 ttctaaagtg atgatcccat tgaagccgga agtggatatc gacgctcttt cgcctgacct
     8581 taatgcgctg accgctatca gtaaacagat tggctgcaat ggtttcttcc cgttccagat
     8641 ccgtccaggc aaaaacgaaa ccgatggtcg tatgttctcg cctgcgattg gtattgtgga
     8701 agatccggtg actggcaatg ccaatggccc gatgggtgca tggttggtac atcacaacgt
     8761 attgccccac gatggcaacg tgttgtgtgt taaaggccat caggggcgcg cattggggcg
     8821 tgacggcatg attgaagtga cggtgacaat tcgtgataac caaccggaaa aagtcaccat
     8881 ttcaggcaca gcggtgattt tgtttcatgc cgaatgggca attgaaatct gaaatcgttg
     8941 cctgatgcgc ttcgcctgtc aggtctacaa ccgggctaat gtatgccgga taaggcgttt
     9001 acgccgcatc cggcaacctg acataaatta ttttcccgcc tccggatgcg tatcaaacgc
     9061 cgccatcacc agcgctaact catccacagt aaagccatgt ttcgcatcat ccacgcccag
     9121 accaaactgt tcctgcatca cggcatataa cgacgccaca tccggtagat ttcgctgctc
     9181 caccgcgtgc ccattttcat aatgggtaaa atgaaaattg gtcagtgtca gcttgccgcc
     9241 gtccggcaaa tggcggcaca tcagcaaatg atggcgaaaa tgcgactgcg gccagtgcgc
     9301 cgaccagaaa ttgcccatca cataatcgct ttgttgctgc tcgcagagat cgaagcggta
     9361 catcggctgc caatgctgat gatgattaaa ctgcaacacc caatcatcac cctcctgcaa
     9421 caaccgatac tctccgtgtg gcgtggtctg cacgagatcg gaaactaaac gaatcggcgc
     9481 ggttagcgtc tgcccaccga aaccgacatc agcaatccat ttttcccctt ccagttccac
     9541 caacagcaaa cgatgggtgc gcggcggtaa tgctggcgga tttgataaca ctacgcgccc
     9601 taacaagctg cgaacgttaa accccagctc gcgtaacacc cgctcaaaca cgccattctg
     9661 ctcaaaacag taaccgccac gacgggctat caccagtttc tcttccagcg attgatcatc
     9721 aagctgtatt tccctcggca gcaaaacgtc aaggttttca aacggaatgg tgcaattgtg
     9781 tttcaggtgc aatgcacgaa gcgtatcaat attgaccgca gcagctcccg accagttaat
     9841 acgggcaaaa tagtgattca gaatgggcgt catcaggcgt ttcccttagt ggttttctca
     9901 acttataacc tggaatagac gccgcaccgg ctattttgtg cgatcgcgga catgcatcac
     9961 gtttgcaaat cgccaatccc gttaatttcg ttacttattg cgttacggac tacgcttaaa
    10021 aaaaccttct tcgcgaggcg ataccatgag ccgattcatc cccatagaat tacaccatgc
    10081 cagccgtctg ttaaatcatg gcccaactgt cttgatcacc agtttcgatg agcaatccca
    10141 gcggcgtaac attatggctg cagcctggtc aatgccggtg gagtttgaac ctccacgcgt
    10201 ggcaattgtg gtagataaat cgacatggac cagggagttg attgaacgta acggtaagtt
    10261 tggcatcgtt atcccgggcg ttgcagcaac taactggacg tgggcggtgg gaagtgtgtc
    10321 ggtgcgtgaa gaagataaat ttaattgcta tggcattccg gtggtgaaag gtccggtgct
    10381 tggtttgcca cttgttgaag agaaatgcct ggcgtggatg gagtgccgcc tgctacctgc
    10441 gacttctgcg caacaaaaat acgacacgct gtttggcgag gtggtatcag cagcggcaga
    10501 cgcacgggta tttgtcgaag gccgctggca gtttgatgat gataagctca atacgttgca
    10561 tcatttaggt gctggcacgt ttgttactag cggcaagcgt gttacggcag gttaagcatt
    10621 acatgctgta accgggtttc tttatcaaag cctccatttg gggctcgatt tcggcatccc
    10681 agatagcttg ccaagattct ggttgaatct gctgtagagc aatgcttatc gagctatctt
    10741 tactgttcag atggcgaata ataacgtcgg taatatctgc agcaagtgct gctttttgtt
    10801 gttcgtccag ttcacgcgga aagcatttaa tgtcgatgtg cggcatgatt acgctccttt
    10861 tgaaagatgt cagtaaaatt aacacacatt gtgaatgtca tgtgtccggt aaagcgttta
    10921 tacgacaata atgcccaggg caagattacg aaagcccgct ccccgtaagg actgacgcca
    10981 ggtagtttct gcccatggct gcttttcgca tcttacgtct taaccctgcc ttgaatacct
    11041 tatcattcgt caaaatatta atagcaatgt gccgtatccc tgaaaataat tctgctgcat
    11101 ttcctcttct tattttgcag tcgtcttcat tcattaccac gtccagacgc caatgcagct
    11161 tattctccac gtgccagtgg tttcggattg ctgtggcgaa cttctctgcg gttaaatcag
    11221 cagaactgat ataatatctg accgtcattt ctggctcttt cttttgttct gctattatgg
    11281 accgaaagga gactgccacg cataatttct tcagcccttt ccattcaaac gtgaaatcaa
    11341 taagttcatc agggacatcg caaacaatat gaagacggat ttcttctctg ccgtgactct
    11401 tttcactaat tgcgtaactg tcattctctg gattatttaa ttctttcagc ggaaattttt
    11461 cctcaaaggc tttatttagc cgcccctggt ttccttttac cgcgaataaa taatcacctc
    11521 cctgtttttg tatcttctct gcaatatctt tctggcaacc catcgcatca gttgtgatga
    11581 tttttccttt aatatccagc atgttaagaa gttcaggaat ggctgtaatc tcattggatt
    11641 tctcatccgt cttaatctgc ccgagaatca gactgtgcat tgttgagaac gcactaatga
    11701 catgaattgc tcccctgcgg cactcttgtc ataagagtgc cggagcgttt ttccatcaat
    11761 tgcaatgacg tctttatcat ctgaagaatg gcagtcacgc atccagttaa taaagcactc
    11821 gtgaaatttt gcaggactga tacaggatac aactctggcg atggtatcgt gaacaggaat
    11881 accattttca aaatcaccat attgttttaa aaaatcgaga tgtgtttccc caaaatcctc
    11941 tatatcttcc cagccttctg caccagaaat aacggcacaa atagtcaaca gtagaatatc
    12001 cgataattta tgttccactt tccaggcttg tttttaatcg gggataatag aaatatgttc
    12061 catcaatttt ttaagttcca ttttgttctc cttaattatg taaggagtat ttgatcatgt
    12121 ataagcaata aaaaacagct tcaggtaata aggaatatct caatttttaa acataaaatg
    12181 cgaattattt agtacaaaaa gtgaactcct cagggcaaga ttacgaaagt ccgctccccg
    12241 caaggactga cgcgagatag tttctgtcca tggctgcttt tcgcatctta cgtcttaacc
    12301 ctgccttgaa taccttatca ttcgtcaaaa cattaatagc aatgtgccgt atccctgaaa
    12361 ataattctgc tgcatttcct cttcttattt tgcagtcgtc ttcattcatt accacgtcca
    12421 gacgccagtg cagattattc tccacgtgcc agtgatttcg aatcgctgtc gcaaacttct
    12481 cagcggttaa atcagcagaa ctgatataat atctgaccat catttcgggc tctttctttt
    12541 gctctgctat tattgagcga aaggagactg ccacgcataa ttttttcagc cctttccatt
    12601 caaacgtgaa atcaataagt tcatcaggga catcgcaaac aatatgaaga cggatttctt
    12661 ctctgccgtg actcttttca ctcattgcgg aactgtcatg cgctggatta tttaattctg
    12721 tcagcggaaa ttttttctca aaggctttat ttagccgccc ctggtttcct tttaccgcaa
    12781 ataaataatc acctccctgt ttttgtatct tctctgcaat atctttctgg caacccatcg
    12841 catcagttgt gatgattttt cctttaatat ccagcatgtt aagaagttct gggatagctg
    12901 taatctcatt agatttctca tccgtcttga tctgtccgat gaccagactg tgcattgttg
    12961 agaacgcact aatgacatga atcgctcccc tgcggcgact cttgtcataa gagtgccgga
    13021 gcgtttttcc atcaattgca atgacgtctt tatcatctgt agaatggcag tcacgcatcc
    13081 agttaataaa gcactcgtga aattttgcag gactgataca ggatacaact ctggcaatgg
    13141 tatcgtgaac aggaat
//
LOCUS       DAEEKQ010000109        13073 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.109, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000109 DAEEKQ010000000
VERSION     DAEEKQ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13073
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.109"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            286..621
                     /locus_tag="KLK57_002910"
     CDS             286..621
                     /locus_tag="KLK57_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HBE0893223.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            779..1222
                     /gene="yjdN"
                     /locus_tag="KLK57_002911"
                     /pseudo
     CDS             779..1222
                     /gene="yjdN"
                     /locus_tag="KLK57_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
     gene            1355..2143
                     /gene="phnC"
                     /locus_tag="KLK57_002912"
     CDS             1355..2143
                     /gene="phnC"
                     /locus_tag="KLK57_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HBE0893224.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVALNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTREQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            2168..3184
                     /gene="phnD"
                     /locus_tag="KLK57_002913"
     CDS             2168..3184
                     /gene="phnD"
                     /locus_tag="KLK57_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0893225.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQGVKSNNGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            3310..4089
                     /gene="phnE"
                     /locus_tag="KLK57_002914"
     CDS             3310..4089
                     /gene="phnE"
                     /locus_tag="KLK57_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HBE0893226.1"
                     /translation="MQTITIAPPKRSWFSLLSWAIVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            4110..4835
                     /gene="phnF"
                     /locus_tag="KLK57_002915"
     CDS             4110..4835
                     /gene="phnF"
                     /locus_tag="KLK57_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HBE0893227.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGIGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            4836..5288
                     /gene="phnG"
                     /locus_tag="KLK57_002916"
     CDS             4836..5288
                     /gene="phnG"
                     /locus_tag="KLK57_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HBE0893228.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLNALNITADYEVIRTAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRHFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            5285..5869
                     /gene="phnH"
                     /locus_tag="KLK57_002917"
     CDS             5285..5869
                     /gene="phnH"
                     /locus_tag="KLK57_002917"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HBE0893229.1"
                     /translation="MTLETAFMLPVQDAQHSFRRLLKAMSEPGVIVALYQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLATPLSNDIVNQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTDTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILHE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            5869..6933
                     /gene="phnI"
                     /locus_tag="KLK57_002918"
     CDS             5869..6933
                     /gene="phnI"
                     /locus_tag="KLK57_002918"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HBE0893230.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLTKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTADSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPDEPPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            6926..7771
                     /gene="phnJ"
                     /locus_tag="KLK57_002919"
     CDS             6926..7771
                     /gene="phnJ"
                     /locus_tag="KLK57_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HBE0893231.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTECTDDAT
                     LIQTRHRIPETPLTEDQIIIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            7768..8526
                     /gene="phnK"
                     /locus_tag="KLK57_002920"
     CDS             7768..8526
                     /gene="phnK"
                     /locus_tag="KLK57_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HBE0893232.1"
                     /translation="MNQPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            8637..9317
                     /gene="phnL"
                     /locus_tag="KLK57_002921"
     CDS             8637..9317
                     /gene="phnL"
                     /locus_tag="KLK57_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HBE0893233.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLIVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIHEAKARGAAIVGIFHDEAVRNDVADR
                     LHPMEASS"
     gene            9314..10450
                     /gene="phnM"
                     /locus_tag="KLK57_002922"
     CDS             9314..10450
                     /gene="phnM"
                     /locus_tag="KLK57_002922"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HBE0893234.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLVNLEKMINAIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARKIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVKLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRKGNHIHIDHVWRQGKRVF"
     gene            10450..11007
                     /gene="phnN"
                     /locus_tag="KLK57_002923"
     CDS             10450..11007
                     /gene="phnN"
                     /locus_tag="KLK57_002923"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HBE0893235.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEQEFFTRAGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            10994..11428
                     /gene="phnO"
                     /locus_tag="KLK57_002924"
     CDS             10994..11428
                     /gene="phnO"
                     /locus_tag="KLK57_002924"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HBE0893236.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFSANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHDAHRFYLREGYEQSHFRFTKAL"
     gene            11430..12188
                     /gene="phnP"
                     /locus_tag="KLK57_002925"
     CDS             11430..12188
                     /gene="phnP"
                     /locus_tag="KLK57_002925"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HBE0893237.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACDRARRSPQYRRQPCSGVV
                     KFNEAITLIDAGLHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDPIPIYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLRNNQPQVMVMDCSHPPRADAPRNHCDLNTVLALNQVIRSPRV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(12335..12664)
                     /gene="yjdP"
                     /locus_tag="KLK57_002926"
     CDS             complement(12335..12664)
                     /gene="yjdP"
                     /locus_tag="KLK57_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HBE0893238.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRRRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(12733..13050)
                     /locus_tag="KLK57_002927"
     CDS             complement(12733..13050)
                     /locus_tag="KLK57_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HBE0893239.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
BASE COUNT         2873 a         3916 c         3674 g         2610 t
ORIGIN      
        1 ttttatcaga ataaataacg tcttctgatg cgtttaaaac gtcagaaaga taaaaatatc
       61 atgtgaataa aaaaaagaac aagtagagca ttaacattat cttaaataat aaatagaggc
      121 aaaaagatta ttttcttttt gcgtttcctt tcaaatgaaa acgatcgtcg tctaaaatca
      181 gcagtacccc cgacaaactc agggattttg tgtataattg cggccttttt cggcaatctg
      241 ccgttttttg gcgcttttgc cctgctgact tttgaggaaa tccacatgtc attaccacac
      301 tgcccaaaat gcaactccga atacacttac gaagataacg gcatgtacat ctgcccggaa
      361 tgtgcctacg aatggaacga cgcagaacct gcacaggaaa gcgacgagct gatcgttaaa
      421 gatgctaacg gcaatctgct ggctgacggc gacagcgtta ccatcattaa agatctgaag
      481 gtgaaaggta gctcttcgat gctgaaaatt ggcaccaaag tgaaaaacat ccgcctggtt
      541 gaaggcgacc ataacatcga ttgcaaaatc gacggttttg gtccgatgaa actgaaatct
      601 gagtttgtga aaaagaactg attgtattgt gatcggtaag ccggataagg cgctcgcgcc
      661 gcatccggca acgatgccga ctgcctgatg cgacgcttgc gcgtcttatc aggcagcctc
      721 tcatctgtat aaatttcgaa ctacacttaa ttggcttctc ttaactgagg tcaccatcat
      781 gccgttaagt ccctacctct cttttgccgg taactgtgcc gacgcaattg cttattatca
      841 acgtacgttg ggcgctgaac tgctctataa aatcagcttc ggcgaaatgc caaaatcagc
      901 gcaggacagc gccgagaact gcccttccgg aatgcaattt cccgataccg ccatcgccca
      961 cgccaatgtg cgcattgccg gaagcgacat catgatgagc gatgccatgc cgtcaggaaa
     1021 agccagctac tccggcttta cgctggtgct cgattcgcaa caggtcgaag aaggaaaacg
     1081 ctggtttgac aatcttgccg ctaacggaaa aatcgaaatg gcctggcagg aaactttcta
     1141 ggcgcatggc tttggcaaag tcaccgataa atttggcgta ccgtggatga ttaatgtcgt
     1201 caaacaacaa ccaacgcaat aacccgccgg gaggcccgcc ctcccgcact gtcatcgaat
     1261 tcccgttaac tcttcatctg ttagtcactt ttaattaacc aaatcgtcac aataatccgc
     1321 cacgatggag ccactttttt agggaggctg catcatgcaa acgattatcc gtgtcgagaa
     1381 gctcgccaaa accttcaatc agcatcaggc gctgcatgcg gttgctctga acattcatca
     1441 cggtgaaatg gtggctctgc ttgggccgtc gggttccggc aaatccaccc ttttacgtca
     1501 cttaagcggt ttgattaccg gcgataaatc cgccggcagc catatcgagc tgctgggccg
     1561 cacagtccag cgcgaaggcc gtctggcgcg cgatatccgc aaaagccgcg ccaacaccgg
     1621 ctacatcttc caacaattca acctggtgaa ccgcctgagc gtactggaga acgtgctgat
     1681 tggcgcgctc ggcagcacgc cgttctggcg cacctgtttt agctggttta cccgcgagca
     1741 gaaacaacgc gcgttacagg cgctgacccg cgttggcatg gtgcattttg cccatcaacg
     1801 cgtttccacc ctctccggcg gacagcagca gcgtgtggcg attgcccgcg cgctgatgca
     1861 gcaggcgaag gtgattctgg ccgatgaacc catcgcctcg ctggacccgg aatccgcccg
     1921 catcgtgatg gacaccctgc gcgacatcaa tcagaacgac ggcatcaccg tggtcgtcac
     1981 gctgcatcag gtggattacg ccctgcgcta ctgcgaacgc atcgtcgccc tgcgccaggg
     2041 gcacgttttc tacgacggca gcagccaaca gtttgataac gaacgttttg accatctcta
     2101 ccgcagcatt aatcgcatcg aagagaacgc gaaagctgcc tgacatcccc atcattgagg
     2161 aaaacgaatg aacgctaaga taattgcctc gctggccttc accagcatgt tcagcctcag
     2221 caccctgtta agcccggcac acgccgaaga gcaggaaaag gcgctgaatt tcggcattat
     2281 ttcaacggaa tcacagcaaa acctgaaacc gcaatggacg ccattcttac aggatatgga
     2341 gaagaagctg ggcgtgaagg tgaacgcctt ctttgcccca gactacgcag gcattatcca
     2401 gggaatgcgc ttcaataaag tggatatcgc ctggtacggc aacctgtcgg caatggaagc
     2461 ggtggatcgc gccaacggcc aggtcttcgc ccagacggtc gcggcggatg gatcgccagg
     2521 ttactggagc gtgttgatcg tcaacaaaga tagtccgatc aacaacctga acgatctgct
     2581 ggcgaagcgg aaagatctca ccttcggcaa tggcgatcct aactccacct ctggcttcct
     2641 cgtccccggt tactacgtct ttgccaaaaa caatatctcc gccagcgact ttaagcgcac
     2701 cgtcaacgcc gggcatgaaa ccaacgcgct ggccgtcgcc aacaagcagg tggatgttgc
     2761 caccaacaac accgaaaacc tcgacaagct gaaaacctcc gcgccagaga agctgaaaga
     2821 actgaaggtg atctggaagt cgccgctgat cccaggcgat ccgatcgtct ggcgcaagaa
     2881 tctctccgaa accaccaaag acaagatcta cgacttcttt atgaactacg gaaaaacgcc
     2941 ggaagagaaa gcggtgctgg aacgcctggg ctgggcaccg tttcgcgcct ccagcgacct
     3001 gcaactggtg ccgattcgcc agctcgcact gtttaaagag attcagggcg taaaaagcaa
     3061 taacggactg aatgagcagg acaagctggc gaaaaccacc gagattcagg cgcagctgga
     3121 tgacctggac cgcctgaaca acgcgttaag cgcgatgagt tcggtgagta aagcggtgca
     3181 gtaatgtgct cgatcggtcc cctctccctt gagggagagg gttagggtga gggggaaatg
     3241 cgcacgctga tgccctcacc ccggccctct cccacaggga gagggagaaa acattaaacg
     3301 gagccaaaca tgcaaaccat caccatcgcc ccacccaagc gcagctggtt ctcgcttctg
     3361 agctgggcca ttgttctcgc cgtgttggtc gtctcgtggc agggcgcgga aatggccccg
     3421 cttacgctga tcaaagacgg cggcaacatg gcgaccttcg ccgccgactt cttcccgccc
     3481 gatttcagcc agtggcagga ttacctcacc gaaatggccg tcacgctgca aatcgccgtc
     3541 tggggcaccg cgctggcagt ggttctctcc atccccttcg gcctgatgag cgccgaaaac
     3601 ctggtgccgt ggtgggttta ccagcccgtt cgccgcctga tggacgcctg ccgcgccatt
     3661 aacgaaatgg tcttcgccat gctgttcgtg gtcgccgtcg gtctcggacc gttcgctggc
     3721 gtgctggcgc tatttattca caccaccggc gtgctctcga aactgctttc cgaagcggtg
     3781 gaagcgattg aacccggccc ggtggaaggc attcgcgcca ccggcgccaa caagctggaa
     3841 gagatccttt acggcgtgct gccacaggta atgccgctgc tgatctccta ctccctctat
     3901 cgcttcgaat ccaacgtccg ctcggcgacc gtcgtcggca tggtcggtgc aggcgggatc
     3961 ggcgtcaccc tgtgggaagc gattcgcggt ttccagttcc aacaaacctg cgccctgatg
     4021 gtgcttatca tcgtcacggt cagcctgctg gatttcctct ctcaacggtt gcgtaagcac
     4081 tttatctgat aagtgaggca ttgatatcta tgcacttgtc tacacatccg accagctacc
     4141 caacacgcta tcaggagata gccgcaaaac ttgagcagga gcttcgtcaa cactatcgct
     4201 gcggcgacta tcttcccgcc gagcagcaac tggcggcgcg ctttgaggta aaccgccaca
     4261 ccctgcgccg cgccatcgac caactggtgg aaaaaggctg ggtacagcgc cgtcagggca
     4321 tcggcgtgct ggtgctgatg cgcccgttcg attacccgct caacgcccag gcgcgtttta
     4381 gccagaacct gctggaccag ggcagccatc ccaccagcga aaaactgctg tcggtattgc
     4441 gccccgcgtc cggtcacgtt gctgacgcgc tggggattac cgagggtgag aacgttatcc
     4501 atttgcgcac cctgcgtcgc gtcaacggcg tcgcgctctg tttaatcgac cactactttg
     4561 cggacctcac cctctggccg acactgcaac gtttcgacag cggttcgctg cacgattttc
     4621 tgcgcgagca aaccggaatt gcgctgcgcc gcagccagac gcggatcagc gcccgccgcg
     4681 cccaggccaa agagtgccag cgccttgaaa tcccgaatat gtcgccgctg ctgtgcgtgc
     4741 gcacccttaa ccaccgtgac ggtgaaagca gcccggcgga gtactccgtc agcctgacgc
     4801 gcgccgacat gattgaattc actatggagc actgaatgca cgcagatacc gcgacccgcc
     4861 agcactggat gtccgtactg gcgcacagcc aaccggctga actggcagcc cgcctgaacg
     4921 cgctgaacat caccgccgac tatgaagtga tccgcactgc cgaaactggc ctggtgcaga
     4981 ttcaggcgcg aatgggcggt accggcgaac gcttttttgc cggcgacgcc acgctgaccc
     5041 gtgccgccgt acgcctgact gacggcaccc tcggctacag ctgggtgctg gggcgtgata
     5101 aacagcacgc cgaacgctgc gcgctgattg atgcgctgat gcagcaatct cgccactttc
     5161 aaaacttatc agaaaccctt attgccccgc tggacgctga ccgtatggca cgcattgccg
     5221 cacgccaggc cgaagtgaac gccagccggg tcgacttctt tacgatggtt cgcggagaca
     5281 acgcatgacc ctggaaaccg cttttatgct tcccgtgcag gatgcccagc acagttttcg
     5341 tcgcctgtta aaggccatga gcgagccggg cgtgattgtc gccctgtatc agctcaaacg
     5401 cggctggcag ccgctgaata tcgccaccac cagcgtgctg ctgacgctgg ccgataacga
     5461 cacgccggtg tggcttgcca cgccattaag taacgatatc gtcaatcaga gcctgcgttt
     5521 tcataccaac gcgccgctgg tcagccagcc ggaacaggcg accttcgcgg tgacggatga
     5581 ggcgatttcc agcgaacagc tcaacgccct ttccaccgac accgccgttg cgccggaagc
     5641 gggcgcgacg ctgattttac aggtcgccag cctgagcggc ggacgcatgt tgcgtctcac
     5701 cggcgcgggc attgccgaag aacgaatgat cgctccgcag ctgccagagt gcattctgca
     5761 cgaactcacc gagcgcccgc acccgttccc gctcggcatc gacctaatcc tgacctgcgg
     5821 cgagcgcctg ctggctatcc cgcgaaccac tcatgtggag gtgtgctgat gtacgttgcc
     5881 gtgaaagggg gcgaaaaggc gatcgacgcc gcccacgccc tgcaagagag ccgacgccgg
     5941 ggcgataccg atttgcccga actgagcgtc gcccagattg aacagcagct taacctcgcg
     6001 gtggatcgcg tgatgacaga aggcggcatt gccgaccgcg aactggcggc gctggcgctg
     6061 aaacaggcca gcggcgataa cgtcgaagcg attttcctgc tacgcgccta ccgcaccacg
     6121 ctgacgaaac tggcggtaag cgagccgctc gacaccacgg agatgcgtct cgaacggcgt
     6181 atttcagcgg tttataaaga tattcccggc ggccagctgc ttggcccaac ctacgactac
     6241 acccatcgcc tgctcgattt cactctgctg gcaaacggcg aagcgccgac gctgaccacc
     6301 gccgacagcg aacaacagcc gtcgccgcac gttttcagcc tgctggcgcg tcaggggctg
     6361 gcgaagtttg aagaggatag cggagcacag ccggatgaca tcacccgcac gccgccggtt
     6421 tacccctgtt cacgttcttc ccgtttgcag cagttaatgc gcggcgacga aggctatttg
     6481 ctggcgctgg cttactccac ccagcgcggt tacgggcgca accacccgtt cgcgggcgag
     6541 atccgcagtg gttacatcga cgtgtcgatt gtgccggaag agctgggatt tgcggtaaac
     6601 gtcggcgaac tgctgatgac cgagtgtgaa atggtcaacg gttttatcga cccgccggat
     6661 gagccgccgc acttcacgcg cggctacggg ctggtattcg gcatgagcga gcgcaaagcg
     6721 atggcaatgg cgctggttga ccgtgcgttg caagccccgg aatacggcga gcacgcgaca
     6781 ggcccggcgc aggatgaaga gttcgtgctg gcgcatgccg acaacgtcga agccgcaggc
     6841 tttgtctcgc acctcaaact cccgcactac gtcgatttcc aggccgaact ggagctactc
     6901 aaacgtctgc aacaggagca gaaccatggc taatctgagc ggctacaact ttgcctacct
     6961 cgacgagcag accaaacgca tgatccgccg cgccatctta aaagcggtgg cgatccccgg
     7021 ttatcaggta ccgtttggcg ggcgcgagat gccgatgccg tacggctggg gaactggtgg
     7081 tatccagctc accgccagcg tgattggcga aagcgacgtg ttgaaggtga ttgaccaggg
     7141 tgcggatgac accaccaacg ccgtgtcgat tcgcaacttc tttaagcgcg tgaccggggt
     7201 aaacaccact gaatgtacgg acgatgcgac gcttatccag acgcgtcacc gcatccccga
     7261 aacgccgctg accgaagatc agatcattat cttccaggtg ccaatcccgg aaccgctgcg
     7321 ctttatcgag ccgcgcgaaa cggagacccg cactatgcac gcgctggagg agtacggcgt
     7381 gatgcaggtg aaactgtatg aagatatcgc ccgcttcggt catatcgcca ccacctatgc
     7441 ttatccggtg aaggtgaacg ggcgctacgt gatggacccg tcgccgatcc cgaaattcga
     7501 taacccgaaa atggacatga tgcccgccct gcagctgttc ggcgcagggc gcgagaagcg
     7561 catctatgcg gtgccgccgt ttacccgcgt ggaaagtctc gatttcgacg atcacccgtt
     7621 caccgttcag cagtgggatg agccatgcgc catctgcgga tcgacccaca gctatcttga
     7681 tgaagtggtg ctggatgacg ccggaaaccg catgtttgtc tgctccgata ccgattattg
     7741 ccgccaacag agcgaggcga aaagccaatg aatcaaccgt tactttcggt caataacctg
     7801 acccaccttt acgcgccggg caaaggcttt agcgatgtct cttttgattt atggccgggg
     7861 gaagtgctgg gcattgtcgg ggaatccggc tccgggaaga ccacgctgct gaagtcgatc
     7921 tccgcgcgtc tgacgccgca gcagggggag attcactatg aaaaccgttc cctctatgca
     7981 atgagcgagg ccgaccgccg tcgcctgctg cgcaccgaat ggggcgtggt gcatcagcat
     8041 ccgctcgacg gcctgcgccg ccaggtgtcg gcaggcggca atatcggcga gcggctgatg
     8101 gcgaccgggg cgcgacatta cggcgatatt cgcgctaccg cgcagaagtg gctggaagag
     8161 gtggagattc ccgccaaccg tatcgacgac ctgccgacca ccttttccgg cggtatgcag
     8221 cagcgtttgc agattgcccg caacctggtg acgcatccga agctggtgtt tatggatgaa
     8281 ccgaccggcg ggctggatgt gtcggtgcag gcccgcctgc tcgacctgct gcgcggactg
     8341 gtggtggagc tgaacctcgc ggtagtgatt gtcacccacg atttaggcgt cgcccgcctg
     8401 ctggcggacc gtttgctggt gatgaagcag gggcaagtgg tggagagtgg gttaaccgac
     8461 cgcgtgctcg acgacccgca tcatccgtat acacagctgc tggtgtcatc ggttttgcag
     8521 aattgagccg gtgccggatg cggcgtaaac gccttatccg gcctacaaat gcgctccccg
     8581 taggtcggat aagacgcgtc agcgtcgcat ccgacacccg aaccacgagg cgaaaaatga
     8641 ttaacgtaca aaacgtcagt aaaaccttca tcctgcacca gcaaaacggc gtgcgcctgc
     8701 ccgtcctcaa ccgcgcctcg ctcatcgtca acgcgggcga atgcgtggtg ctccacggcc
     8761 attccggcag cggaaaatca accctgctac gctcgctgta cgccaactat ctgcccgacg
     8821 aagggcaaat ccagatcaaa cacggtgacg aatgggtcga tctggtcacc gcgccagcgc
     8881 gcaaagtggt ggagatccgc aaaaccaccg ttggctgggt gagccagttt ctgcgcgtca
     8941 tcccgcgtat ttcggcgctg gaagtggtga tgcagccgct actcgatacc ggcgtaccgc
     9001 gtgaagcctg cgccgctaaa gccgcgcgtc tgctcacccg cctgaacgtg ccggaacgcc
     9061 tgtggcatct ggcgccatcg accttctccg gcggcgaaca gcagcgcgtc aacatcgccc
     9121 gcggctttat cgtcgactac cccattctgc tgctcgacga acctaccgcc tcgctggacg
     9181 ccaaaaacag cgccgccgta gtcgagctga ttcacgaagc caaagcccgt ggcgcggcca
     9241 tcgtcggcat cttccacgac gaagccgtac gtaatgacgt tgccgaccgc ctgcacccaa
     9301 tggaagcctc ttcatgatta tcaataacgt caaattggtg ctggaaaacg aagtggtaca
     9361 cggttcactg gaggtgcagg acggcgaaat ccgcgccttt gccgaaagcc agagccgcct
     9421 gccggaggcg atggacggcg aaggcggctg gctgctgccg gggctgattg agctgcatac
     9481 cgataatctg gataaattct tcaccccgcg cccgaaagtt gactggcctg cccactcggc
     9541 gatgagcagc cacgacgcgc tgatggtggc gagcggcatc accaccgtac tggatgccgt
     9601 ggcaattggc gacgtgcgcg acggcggcga tcggctggtg aatctggaga agatgatcaa
     9661 cgccatcgaa gagacgcaga aacgcggcgt caaccgcgcc gagcaccgtc tgcatctgcg
     9721 ctgcgaactg ccgcatcaca ccacgctgcc gctgtttgaa aaactggtgc agcgcgagcc
     9781 ggtgacgctg gtgtcgctga tggaccactc gccgggccag cgccagttcg ccaatcgcga
     9841 gaagtatcgc gaatattatc agggcaaata ctccctcact gatgcgcaga tgcagcagta
     9901 cgaagaagag caactggcgc tcgccgcacg ctggtcgcag ccgaatcgcg aatccatcgc
     9961 cgccctgtgc cgcgcgcgaa aaattgcgct tgccagccac gatgacgcca cccacgccca
    10021 cgttgctgaa tctcaccagc ttggcagcgt gatcgccgaa tttcccacca cgttcgaagc
    10081 ggcggaagcc tcgcgcaagc atggcatgaa cgtgctgatg ggcgcgccga atattgtgcg
    10141 cggcggctcg cactccggca acgtggcggc cagtgaactg gcgcagcttg gcctgctgga
    10201 tatcctctct tccgactact accccgccag cctgctcgat gcggcatttc gcgtcgccga
    10261 tgacgagagc aaccgcttta cgctgccgca ggcggtgaag ctggtgacta aaaatccagc
    10321 gcaggcgctt aatctccagg atcgcggggt gattggcgag ggcaaacgcg cggacttggt
    10381 gctggcgcat cgcaagggca atcacattca tatcgaccac gtctggcgtc agggtaaaag
    10441 ggtgttctga tgatgggaaa actgatttgg ttaatggggc cgtccggctc cgggaaagat
    10501 agcctgctgg cggaactccg cctgcgggaa caaactcagc tactggtggc gcatcgctac
    10561 atcacgcgcg acgccagcgc cggaagcgaa aaccatatcg ctctgagcga gcaggagttt
    10621 tttacccgcg cggggcagaa cctactggcc ttaagctggc acgctaacgg tctgtattat
    10681 ggcgtcggcg tcgagatcga cctctggctg cacgccggat tcgacgtgct ggtcaacggc
    10741 tcacgcgccc atctgccgca ggcgcgggcg cgctatcaat cggcgctgct gcccgtctgt
    10801 ttacaggttt cgccggagat cctccgccag cgcctggaaa accgtggccg tgaaaacgcc
    10861 agtgaaatta acgcccgcct ggcgcgcgcc gcccgctata ctccacagga ttgccatacg
    10921 ctcaacaatg acggcagcct gcgccagtcg gtcgacacgc tgctgacgct gattcatcag
    10981 aaggagaaac accatgcctg cttgtgagct tcgcccggcc acgcagtatg ataccgacgc
    11041 ggtttacgcg ctgatttgtg agctaaaaca ggcggagttt gaccaccacg catttcgcgt
    11101 gggctttagc gccaatctgc gcgacccgaa catgcgctat catctggcgc tgctggatgg
    11161 cgaggttgtc ggcatgatcg gcctgcattt gcagtttcat ctgcatcatg tcaactggat
    11221 cggcgaaatt caggaactgg tagtgatgcc gcaggcgcgc ggcctgaacg tcggtagcaa
    11281 gttgctggcg tgggcagaag aagaagcccg ccaggccggg gccgaaatga ccgagctttc
    11341 gaccaacgtg aagcgccacg acgcgcaccg tttctatctg cgagaaggct acgagcagag
    11401 tcacttccgc ttcaccaagg cgctgtaaca tgagtctgac cctcacgctc accggcaccg
    11461 gcggcgcaca gggcgttccg gcatggggct gcgagtgcgc ggcctgcgac cgagcgcggc
    11521 gctcgccgca gtatcgccgc caaccgtgca gcggcgtagt gaagtttaac gaggcgatca
    11581 ccctgatcga cgccgggctg cacgatctca ccgatcgctg gtctcccgga tcgttccagc
    11641 agtttttgct gacgcattat catatggatc acgtccaggg gctgtttccg ctgcgctggg
    11701 gcgtgggcga tccgatcccg atctacggcc cgccggatga acagggctgc gacgatctgt
    11761 ttaaacatcc cgggctgctt gatttcagcc acacggtgga accgtttgtg gtgtttgatt
    11821 tgcaggggtt gcaggtcacg ccgctgccgc ttaaccactc aaaactcact ttcggctatt
    11881 tactggaaac ggcgcacagc cgggtggcgt ggctgtctga caccgctggc ttgccggaaa
    11941 aaacgctgaa atttttacgc aataatcagc cgcaggtaat ggtgatggat tgcagtcacc
    12001 cgccgcgcgc ggatgcaccg cgtaatcact gtgatttaaa taccgtgctt gcgctgaatc
    12061 aggttatccg ctcgccacgg gtgattctga cccatatcag ccaccagttt gatgcgtggc
    12121 tgatggaaaa cgcactaccg tcagggtttg aggtggggtt tgatgggatg gagattgggg
    12181 tggcgtgatg agagggaatg tgcgcgctgg ccccctcacc ctaaccctct ccccagaggg
    12241 gcgaggggac cgattgtgct caatattgaa tattgcgctc gttttctccc tctccccatt
    12301 ggggtgaggg gcgatgcctg ctccataccc aacctcatcg cccatactca tcttccattc
    12361 tccgctcttc atcctccagt tgccgacgct cctgatcaag ctggcgctgg cgatcgtcca
    12421 gctgcctgcg gcgatcttca aactggcggc ggcggtcgtc atattgtctg cgccgatcgt
    12481 cgctcacttc acgctgccag ccgtcgtcgc gcgaatcttc atagtctcgc ccacggtcag
    12541 ggttataagc gtcattaatc gcctgctgaa tattaccaat agtatcgtcg ataatatcag
    12601 cctgggccgg aacggtggac agcgtgagca gggtgaataa aagaaatagc ggaaagcgtt
    12661 tcattagcca acctcaaaaa gaaactctat ccacattagt cattactcat ccatgcaagt
    12721 agtggatgaa tctcaatttc tccgctgctc tattgccgta atcgcctcca cgcgttgttg
    12781 atgacgaccg ccttcgtact gtgcgcccag ccacgcatcc acaatcattt ttgccagttc
    12841 gaggccaacc actcgtgaac caaaagccag tgcaacttca ctggcataat gcggataatc
    12901 agtgcgctct gacgacctgg tgtctttatc aatcacctca ctcattgacc atgaataaaa
    12961 ggctggcaca gcaacgcaga atcctgcggt gccagcgaca tgaccgccca ttgctccggt
    13021 acgtttttta aaaagaaaca ccggttgcat tgataaattg tgctcagtat ttg
//
LOCUS       DAEEKQ010000110        13053 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.110, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000110 DAEEKQ010000000
VERSION     DAEEKQ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13053)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13053)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13053
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.110"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(344..1132)
                     /gene="phoH"
                     /locus_tag="KLK57_002928"
     CDS             complement(344..1132)
                     /gene="phoH"
                     /locus_tag="KLK57_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HBE0893240.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGGGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1753..3024)
                     /gene="efeB"
                     /locus_tag="KLK57_002929"
     CDS             complement(1753..3024)
                     /gene="efeB"
                     /locus_tag="KLK57_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HBE0893241.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHV
                     SRSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFSLPGVKDANDYLGSALLRV"
     gene            complement(3030..4157)
                     /gene="efeO"
                     /locus_tag="KLK57_002930"
     CDS             complement(3030..4157)
                     /gene="efeO"
                     /locus_tag="KLK57_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HBE0893242.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(4215..5045)
                     /locus_tag="KLK57_002931"
     CDS             complement(4215..5045)
                     /locus_tag="KLK57_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HBE0893243.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGIFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(5711..7219)
                     /gene="putP"
                     /locus_tag="KLK57_002932"
     CDS             complement(5711..7219)
                     /gene="putP"
                     /locus_tag="KLK57_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HBE0893244.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALLAGAASTILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGDSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARRISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKQASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQEN"
     gene            7641..11603
                     /gene="putA"
                     /locus_tag="KLK57_002933"
     CDS             7641..11603
                     /gene="putA"
                     /locus_tag="KLK57_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HBE0893245.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLENLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLVDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVVDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELHKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(11643..12281)
                     /gene="rutR"
                     /locus_tag="KLK57_002934"
     CDS             complement(11643..12281)
                     /gene="rutR"
                     /locus_tag="KLK57_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HBE0893246.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            12569..>13053
                     /locus_tag="KLK57_002935"
     CDS             12569..>13053
                     /locus_tag="KLK57_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBE0893247.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPVYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     D"
BASE COUNT         3170 a         3604 c         3293 g         2986 t
ORIGIN      
        1 tcagaggaat atattcggtg ttaaacagtc agtcaaagta tagcccattg ttgattaaac
       61 ctcataagca aaattggcac cagatctcct gtacaggttg aatggccgaa aaaagatagg
      121 cagatgctca tttttttggc ttctggaaga aaaactctcc gcgctggcag tattacactg
      181 gtctgatgct atgacgaaaa atgcctggta agagtatcta aaccagactc cagttcagct
      241 tatcaacacg ctcaaagtcg cccccctgaa cgctaacctg aagtgaaaat ctgttgaaat
      301 aaaacaaagc ccggttcgcc cgggctctgc accgataaca cacttaactg taggcatgca
      361 gcgtacgttg gcaaagtgcc gaacgtacgc agtcctcttt accgaaccgg acgatcccaa
      421 ccatttcatc ttcttcgaaa cgttccagcg cgtcacttaa tccggagcac acgccgcgag
      481 gcaaatcgca ttgcgtgata tcaccgttga cgataaccgt cacgttctcc ccgaggcggg
      541 ttaaaaacat tttcatttgc gcggcagtca cattctgcgc ctcgtcaaga atgacgactg
      601 cattttcaaa ggtacgtcca cgcatatagg cgaacggcgc aatttccacc ttccctattt
      661 ccggtcgcag gcagtactgc ataaaggaag cccctaagcg ccggaccagc acgtcgtaga
      721 ccgggcgaaa atagggagca aacttttctg cgatatctcc aggtaagaag ccaagatctt
      781 catcggcttg cagaactgga cgggtgacga taatcctgtc gacatcctta tgtatcaggg
      841 cctctgccgc ttttgctgcg ctgatccagg tttttccgcc cccggcttcg cccgtggcga
      901 atatcagctg cttactctca atagccttca gatagtgcaa ttgcgcttca tttcgcgcga
      961 ggatgggcga agtatcgcga ctgtcgcggg ccataccaat ggcttctacg ccgcccatct
     1021 gcacaagcga ggtgaccgat tcttcttcac gctgcttatg gctgcgcgaa tcccgtctca
     1081 gcacacgttt tgcctcgcga cgagctttga tcactgcttt ttgtcttccc atagagagca
     1141 ccttgagttg tttgtattca tcacacgcgc cgttggcagc gcgattatgc gcacgaacat
     1201 cagagggttg gcttccttgt aagccatagt ttgcttttgg ataaaatgcc gaaaaacggc
     1261 tacgcgcacc gtttacggcg tcggtaacac atgaaaagaa aggatgaggt tgaaaatgca
     1321 aagtgacgag atgactaccg gaggagaaaa ctccgcgagt ggtggcgcgt tgattatcta
     1381 aaacatgtcc agtacaggac gttaccatcc gcgatctcca tagtgactga ctatcactgc
     1441 cgggaacttc cgctgctact taataagtac aacagatctc gcatttattg caacaatata
     1501 tttacttata tttaactata aaacactatt tcagtgacat tagtttctac tggaaagatg
     1561 acagagtgat gacagtgatg aaaaaagctg tgtgatttca gcaggatttg catcaggcat
     1621 cagtgcacga tcgccggatg cggcgtgaac gccttatcca gcctaccaaa cccgcactga
     1681 ttgtaggcat gataagacgc gcaagcgtcg catcaggcat ctgcgcacaa atgccttatc
     1741 cgcctaaaaa cattaaaccc gcaataacgc gcttccgaga taatcgttcg cgtccttcac
     1801 tcccggcagc gaaaaaaaat aaccgccgcc gataggttta acgtattcct ccagcgcttc
     1861 gccattgagc cttttttgta ctgtcaggaa gcctttttcc agatcgtgtt ggtagcagac
     1921 aaacagcaac cccatatcca gttgtccgga gttggtgacc cccaacgaat aactgtagcc
     1981 gcgacgcagc atcaggctgg actcactctc cgccgtgcgg ggattcgcca gccggatatg
     2041 gctgtccagc gcaatcacct tcccttccgg gtcgctggcg taatcaggca catcatgctc
     2101 atgctgcatt cccagcggcg caccggtttg cttatcacgg ccaaaaatcg tctgctgttc
     2161 tttcagcggc gttctgtccc aaaattccac tcgaaactga atcaagcgta ctgcctgata
     2221 gctgccaccg attgtccacg caggctcctg ctgatctgcc gttacccaca ccactttttg
     2281 catcaactta tcattctggc tatcgggatt ggcagtgccg tctttgaaac ccagcaaatt
     2341 aatcggcgtc tctttgcctt tactacgcga cacgtgatcg gaaataaacc cttcccgctt
     2401 ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata tcgcgcagcg catggataac
     2461 cgtgtcctgg gtgttggcgc aaatctgtag caacacatca ccatgacata acgccgcatc
     2521 cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt tttggcatct gtggcgcaag
     2581 gccaaagcgc tcatcaaaca atgagtgacc caccgataac gtgatggtga gattatcggg
     2641 cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc aggcgcggat ttggcgtttc
     2701 tggtgctgct ccgccctgag tcagaaaagc aaaacgctga gtcaacaagc gaaacaaccg
     2761 ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc accagcatca ttgcggcctg
     2821 ttgtggcgtc aggatccctg cctgatgctc accataaaac ggctgtttct cattgcgtgc
     2881 atccggtgaa agcgtgcccg gtgcactttg cgttttttgt gcatgagcga ccggacaact
     2941 tcccgccagc gccagcgcac caatcccttt cagtaaacgt cggcgtgacg gttcattcac
     3001 gccgttttca tctttatact gcataacgct taatccagtc ccagcacacc gcgaagttgc
     3061 gccagatctt ccgccagcgc agtaatcggt cctttcagtg cattccggtc ggcatcggtc
     3121 aatttgtcgt aggtttcaaa accgtcttta gtacggtatt tcgccagaat ggtatcgacc
     3181 tttttaaagt tggcatcgac ttttgccagc agttccgggt tggctttttg cagttgtgga
     3241 cgcagcaaat cgacaatttt ctgcgagcct tcaacgttag cctggaaatc ccacagatcg
     3301 gtgtggctgt agcgatcttc ttcaccgcta attttgctgg ctgccacttc ctcaatcagt
     3361 ccggctgcgc cgccgaccac ttttgaaggt gggaaagcca gttcactgat gcgtttttgc
     3421 aaatcgacca catctgtata aagctgctca gcgtactgat ccatcccttt ggtggtgttg
     3481 tcgccaaaca atgctttttc cagacggtgg aaaccagtga attttgggtc ggcggctttt
     3541 tgctcgtaat catcttcacg ggcgtcaatg ctgccatcca gatcggagaa cagttcagca
     3601 atcggttcaa tgcgctcata gtgctggcgc gtcggcgcat acagtgcttt cgctttttcg
     3661 atatcgcctg ctttaatcgc gtcggtaaag gctttggtgt cggtcaccag ctgcgtggtt
     3721 tccgccatga catacgcttt atatgcagta attgcaccac caagacttaa cagcgcatca
     3781 ctttgcgccg catccgccgt tgcctcacct ttgacgatca acttcccttt cgggttagtc
     3841 agcagaccgc aggtcatatc gtattcgcca ggctgtaaat tcgccgtcat tttctggcta
     3901 aaaccagggg cgatattttc ccgctcttcc accaccatca cgcctttgag gatctcccac
     3961 tccagcgcct tctggctatg gttctgaata atgaactgtg ttttcccggc gttaaccgta
     4021 atggtcatcg gttcgcactg cttatccgtc acggtcactt tgacctgcgg cacatcagcg
     4081 gcgttagcca taaaagcaga agaaaacagc gcagccacgc tcaactgcaa tgcgttacgg
     4141 cggaagttaa tggtcatgac gagactatcc ctttaaagta tgttgtaact aagcaagagt
     4201 ttgcgtcgta tttgttacgc ggagcgagac gctgtcgccc ctgcgcgtgg tggcagagca
     4261 aatgccacca gcgccgggat gagataaata aaccagacgg cgacttcgct gacgctcggt
     4321 gcttcctgat agccaaaaat cccttccatc agcgtgccaa acagcgagtg agttgagagc
     4381 actgcgctca tatcgaaggc gatttcctga aagtggttcc acaatccggc ttcatgaaat
     4441 gcgcgaatgg caccagcagc cagccctgcg gcgacgaaga gaataaacag gctggtccat
     4501 ttaaaaaatg caccaagatt gaggcgaata ccgccccagt agagcaggaa gcctagcacc
     4561 acggcagtag caagaccgag cattgcaccc agcggcggcc agatcccgac atcttgttga
     4621 aatgccgcca gcaggaaaaa gaccgactcc agcccttccc ttgcaacggc aaaaaagacc
     4681 atcatcacca gcgcccagcc atgatgattt ccacgctgca atgcgctatc gactgcctgc
     4741 tccagttgta ctttgacgtt gcgcgacact ttgcgcatcc agaaaaccat ccaggtaagg
     4801 atcaccacgg cgatcaccgc cacgatacct tcaaacagtt cctgttcttt ttgcggaaat
     4861 tcgccggtgg tttcgttaat gaagataccc aagcccaggc acaacgcagc ggcaagcaac
     4921 acgccaatcc acatcacgcc aatccatcgg cctcgctggg tacgcttaag atagctggca
     4981 atcaaactga caatcagcgc ggcttcaagt ccttcgcgca acataatgag aaacggaaca
     5041 aacatgccac ccacccttaa acgtttttct gagtcaatcc atgcaaagaa aagtaaatcg
     5101 cagtgatagc gattatcatt acggcaaaga aaaatacaag cggaatgttt ataaaatgat
     5161 gttggaatgt aaacaaagag aggtaatacc aggggatata aacgattatt ccaggtacaa
     5221 agatgggatt gtttacaacg agccatgtgt tcttgccaga tgccgcgtaa acgccttatc
     5281 cagccgacaa aactgtccag actcaatgca tcacagcttc catgtaaaac tgataagcgg
     5341 gtgtatcaag caatttcgtc catccgcgat tatcaaatct aactttttgc ccggaacata
     5401 ataacagcgc gggtgttggt ggcgtcaaaa tcgctgaaac gttccccgaa ggccggggca
     5461 gcccacatgg atgtgggctg agggcgcgtc ttacaggacg ttacctcgcg cccgacccgg
     5521 tagccgaagg ggataagctg aaggtaccgc gcagcggcga ttttgttcgc cagagcccgg
     5581 gggggcaggg ggcggcggcg actggccgcc ccctgcgcgc tccctgcacc attcaattta
     5641 tatggcgagg cacattaagg gagcgcaacc cgctgctgaa gccaaacaaa accgccgcag
     5701 gctaagtccc ttagttttcc tgcaaccgtg acggcggagc cgaatgatag tgcgcatcgg
     5761 cctcggcaaa gcgtttttgc atcgccgctg acggcgcttt acccagcaaa ctaaacacta
     5821 caatcccgat actgccgaag atgaagcccg gaataatttc gtacagtccc agccagccga
     5881 actgcttcca gacgataact gtcagcgcac cgatgatcat ccccgccagc gcaccgttgc
     5941 gcgtcatgcg tgaccacatc accgagaaca gcaccaccgg accaaacgcc gcgccaaagc
     6001 ctgcccacgc gtagctcact aagcccagca cgcggttttc cgggtttgcc gccagcgcaa
     6061 tcgccaccag cgccaccacc agcaccatca cacgtcctac ccacaccagc tctttctggc
     6121 tggcctgttt acgcagaaac gctttgtata aatcttcggt aatcgcactg gagcacacca
     6181 gcagctggca acttaaggtt gacattaccg ccgccagaat cgccgacagc agaatcccgg
     6241 caatccacgg gttaaacaga atttgcgcca gttcgataaa cacgcgttca gcgttctggt
     6301 ttaccgcacc tgctaccgcc ggatgctcgt taaagtaagc gataccaaag aagccaacag
     6361 ccaccgcccc tgccaggcaa aggatcatcc aggtcatact gatacgacgc gcatggacaa
     6421 tgctgtggtg agaatctgcc gccataaaac gcgccaggat gtgcggctgc ccgaagtaac
     6481 ccagccccca acccatcaat gagataatgg caacaaagtt cagccctttg agcatatcca
     6541 cgttttcgat gctcttttgt ttgatcactt ccagcgagtc accaaagcca ccgacactga
     6601 taatgacgat aaccggcgtc aggatcaggg caaaaatcat caggctggcc tgtacagtgt
     6661 cagtccagct caccgcgagg aaaccgccaa taaaggtgta aaggatcgtc gacgcagctc
     6721 ccgccaacag agccgtttcg taactcatgc caaaggtact ttcaaacaga cgcgcgcctg
     6781 ccacaatgcc cgaagcgcaa taaatggtga agaacagcaa aataaccagc gcggagataa
     6841 tgcgcaaaat gcggctttta tcttcaaaac gcccggtgaa ataatccggc aatgttaagg
     6901 cgttattatt gtattcggta tgcacacgca accgcccggc caccagcttc cagttgatcc
     6961 acgcgcctaa tgtcaggcca atggcgatcc agctttcgga aatcccggaa agaaaaaccg
     7021 cgccaggcaa ccccattaac agccagccgc tcatatccga cgcacccgcc gataatgccg
     7081 tcacgaatgg cccaagacta cgaccgccca gaatatagtc gtcaaagttt ttcgttgatc
     7141 gccaggcgat aaacccaatc aatatcatgc caaagatata gacacaaaat gtcaccaaca
     7201 tcggtgtgct aatagccatc taaagtctcc aaaaaattat tatcggcaat gtcgaaactt
     7261 gccgttatat ctgccaccgg aacggggtaa cagagtttat gttttaccag ggcgaccgta
     7321 tcctgccgga agcgctggtt attcacaatc gatttaacac accatttaca ttaaatttta
     7381 gtgctcagcg acactatttt tcatcaggtt gcactctctc acattttttg cggttgcacc
     7441 tttcaaaaag gttaactgcc gcagagaaaa agtctgagtt attttttaat cactgtcata
     7501 tcgatttctt ttattaacat ttcattcatt tttaagcttg ctacgcaagt cacatttaac
     7561 atggttgcac aaagttgcaa catcatggat atttcacgat aacgttaagt tgcacctttc
     7621 agaacaacag gagtaatggc atgggaacca ccaccatggg ggttaagctg gacgacgcga
     7681 cgcgtgagcg tattaagtct gccgcgacac gtatcgatcg cacaccacac tggttaatta
     7741 agcaggcgat tttttcttat ctcgaacaac tggaaaacag cgatactctg ccggagctac
     7801 ctgcgctgct ttctggcgcg gccaatgaga gcgatgaagc accgactccg gcagaggaac
     7861 cacaccagcc attcctcgac tttgccgagc aaatattgcc ccagtcggtt tcccgcgccg
     7921 cgatcaccgc ggcctatcgc cgcccggaaa ccgaagcggt ttctatgctg ctggaacaag
     7981 cccgcctgcc gcagccagtt gctgaacagg cgcacaaact ggcgtatcaa ctggccgata
     8041 aactgcgtaa tcaaaaaaat gccagtggtc gtgcaggaat ggtccagggg ttattgcagg
     8101 agttttcgct gtcatcgcag gaaggcgtgg cgctgatgtg tctggcagaa gcgttgttgc
     8161 gtattcccga caaagccacc cgcgacgcgt taattcgcga caaaatcagc aacggtaact
     8221 ggcagtcaca cattggtcgt agcccgtcac tgtttgttaa tgccgctacc tgggggctgc
     8281 tgtttactgg caaactggtt tccacccata acgaagccag cctctcccgc tcgctgaacc
     8341 gcattatcgg taaaagcggt gaaccgctga tccgcaaagg tgtggatatg gcgatgcgcc
     8401 tgatgggcga gcagttcgtc acgggtgaaa ccatcgcgga agcgttagcc aatgcccgca
     8461 agctggaaga gaaaggtttc cgttactctt acgatatgct gggcgaagct gcactgactg
     8521 ccgcagacgc acaggcgtat atggtttcct atcagcaggc gattcacgcc attggtaaag
     8581 cgtctaacgg tcgtggcatc tatgaaggtc cgggcatttc aatcaaactg tcggcgctgc
     8641 atccgcgcta cagccgcgcc cagtatgacc gggtaatgga agagctttac ccgcgtctga
     8701 aatcactcac cctgctggcg cgtcagtatg atattggtat taacatcgat gccgaagagg
     8761 ccgatcgtct ggagatctcc ctcgatctgc tggaaaatct ctgtttcgag ccggaactgg
     8821 caggctggaa cggcatcggt tttgttattc aggcttatca aaaacgctgc ccgttggtga
     8881 tcgattacct ggttgatctc gccacccgca gccgtcgccg tctgatgatt cgcctggtga
     8941 aaggcgcgta ctgggatagt gaaatcaagc gtgcgcagat ggacggcctt gaaggctatc
     9001 cggtttatac ccgcaaggtg tataccgacg tttcttatct cgcctgtgcg aaaaagctgc
     9061 tggcggtgcc gaatctaatc tacccgcagt tcgcgacgca caacgcccat acgctggcag
     9121 cgatttatca actggcaggt cagaactact atccgggtca gtacgagttc cagtgcctgc
     9181 atggtatggg cgagccactg tacgagcagg tcaccgggaa agttgccgac ggtaaactta
     9241 accgaccgtg tcgtatttat gccccggttg gcacacatga aacgctgctg gcgtatctgg
     9301 tgcgtcgcct gctggaaaac ggtgctaaca cctcgtttgt taaccgtatt gccgacacct
     9361 ctttgccact ggatgaactg gtcgccgatc cggtcactgc tgtagaaaaa ctggcgcaac
     9421 aggaagggca aactggatta ccgcatccga aaattcccct gccgcgcgat ctttacggtc
     9481 acgggcgcga caactcggca gggctggatc tcgctaacga acaccgtctg gcctcgctct
     9541 cctctgccct gctcaatagt gcactgcaaa aatggcaggc cttgccaatg ctggaacaac
     9601 cggtagcggc aggtgagatg tcgcccgtta ttaaccctgc ggaaccgaaa gatattgtgg
     9661 gctttgtgcg tgaagccacg ccacgtgaag tagaacaggc gctggaaagt gcggttaata
     9721 acgcgccaat ctggtttgcc acgcctccgg ctgaacgcgc ggcgattttg catcgcgctg
     9781 ccgtgctaat ggaaagccag atgcagcaac tgattggtat tctggtgcgt gaagccggaa
     9841 aaaccttcag taacgccatt gccgaagtgc gcgaagcggt cgattttctc cactactacg
     9901 ccggacaggt gcgggatgat ttcgctaacg aaacccaccg tccattaggg cctgtggtgt
     9961 gtatcagtcc gtggaacttc ccgctggcta ttttcaccgg gcagatcgct gccgcactgg
    10021 cggcaggtaa cagcgtgctg gcaaaaccgg cagaacaaac gccgctgatt gccgcgcaag
    10081 ggatcgccat tttgctggaa gcgggtgtac cccctggcgt ggtgcaattg ctgccaggtc
    10141 agggggaaac cgtgggcgcg caactgacgg gtgatgatcg cgtgcgcggg gtgatgttta
    10201 ccggttcaac cgaagtcgct acgttactgc agcgcaatat cgccagccgc ctggacgctc
    10261 agggtcgccc tattccgctc atcgctgaaa ccggcggcat gaacgcgatg attgtcgatt
    10321 cttcagcact gaccgaacaa gtcgtcgtgg atgtgctggc ctcggcgttc gacagtgcgg
    10381 gtcagcgttg ttcggcactg cgcgtgctgt gcctgcaaga tgagattgcc gaccacacgt
    10441 tgaaaatgct gcgcggtgca atggccgaat gccggatggg taatccgggt cgcctgacca
    10501 ccgatatcgg tccggtgatt gatagcgaag cgaaagccaa tattgagcgc catattcaga
    10561 ccatgcgtag caaaggccgt ccggtgttcc aggcggtgcg ggaaaacagc gaagatgccc
    10621 gtgaatggca aagcggcacc tttgtcgccc cgacgctgat cgaactggat gactttgccg
    10681 aattgcacaa agaggtcttt ggtccggtgc tgcatgtggt gcgttacaac cgtaaccagc
    10741 taccagagct gatcgagcag attaacgctt ccggctatgg tctgacgctt ggtgtccata
    10801 cgcgtattga tgaaaccatc gcccaggtca ctggctcggc ccatgttggt aacctgtatg
    10861 ttaaccgtaa tatggtgggc gcagtggttg gtgtgcagcc gttcggcggc gaagggttgt
    10921 ccggtaccgg gccgaaagca ggcggtccgc tctatctcta ccgtctgctg gcgaatcgcc
    10981 cggaaagtgc gctggcagtg acgctcgcgc gtcaggatgc agagtatccg gtcgatgcac
    11041 agttgaaagc cgcattgact cagccgctaa atgcactgcg ggaatgggca gcaaatcgtc
    11101 cagaattgca ggcgttatgt acgcaatatg gcgagctggc gcaggcagga acacaacgat
    11161 tgctgccggg gccgacgggt gaacgcaaca cctggacgct gctgccgcgt gagcgcgtgt
    11221 tgtgtattgc cgatgatgag caggatgcgc tgactcagct cgccgccgtg ctggcggtgg
    11281 gcagccaggt actgtggccg gatgacgcgc tgcatcgtca gctagtaaag gcattgccat
    11341 cggcagtcag cgaacgtatt caactggcga aagcggaaaa tataaccgct caaccctttg
    11401 atgcggtgat cttccacggt gattcggatc agcttcgcgc attgtgtgaa gccgttgctg
    11461 cgcgggatgg cgcaattgtt tcggtgcagg gttttgcccg tggcgaaagc aatatccttc
    11521 tggaacggct gtatatcgaa cgttcgctga gtgtgaatac cgctgccgct ggcggtaacg
    11581 ccagtttaat gacgatcggt taaacaggta gccggaggat gttacaacct cctccggcat
    11641 ctttaacgtg gtcgaatccc ctcaataata atccgctgca cgttttcaac cgtttgattg
    11701 aaaaatatct catcgcgcaa cgtcgcgcct gtcaccgcct ccacctgagg ggcgaaatcg
    11761 gcgtaatgtt gagtggaagc ccaaatcatg aaaatcaaat gctgcggatc aatcggcgcg
    11821 agtttgccgc ttttgaccca accggcaatc agcgcagatt tctcatcaat taatgccttc
    11881 aaatcgcccg tcagttcatc cattaacagc ggcgcgcctg ccagcatctc catacagaac
    11941 aggcgcgaag cctgcggata atcgcgggag acttccagct tcagacggat gtactctttt
    12001 atcgccgcca gcggagcgaa atcttcacga aacgctttta acggtgccag ccagatatcg
    12061 agaatctgcc gcagcacggc aatatacagc gcctcttttg acggaaagta atacagcaga
    12121 ttggtttttg aaacacccgc caactctgcg atctgctcca gccttgtgcc gtgaaaaccg
    12181 aattgtgaaa aagtgtccag tgctgcgcta agaatcgctt ttttcttcgc gcttactgcg
    12241 cgcgaacgtt tacccgttgt tttcactgcg ccttgcgtca tgcgctctcc cctcttgtag
    12301 attggcaaca ggatagcaaa acccccgccc ggcctcgacc ttctgcactc tcatcgcgca
    12361 cagatgcatg ttttatgtgc aactgttttg accgtttagt ccacttttta tcagatattt
    12421 tagataacgt aatattaaca tattaataac attaactttt taaaaactgg catccgcttt
    12481 gcaaacaagc caatacgcgg ctgaaaagga tatgcaggat gcagcgcccc gtctgacttt
    12541 cactttacga gacgaagaga ggttgatgat gaaaattggc gtattcgtac ctattggcaa
    12601 caacggctgg ctcatttcga cccacgcgcc gcagtacatg ccgacctttg aactgaataa
    12661 agccattgtg cagaaagcgg agcattacca tttcgatttc gccctgtcga tgatcaaact
    12721 gcgtggcttt ggcggcaaaa ctgagttctg ggatcacaac cttgagtcgt tcaccttgat
    12781 ggcgggtctg gcagccgtga cctcgcgcat tcagatttac gccactgctg ccaccttaac
    12841 gttacctccg gcaatcgtcg cccgtatggc cgcaaccatc gactccatct ctggcgggcg
    12901 ttttggcgtc aacctcgtga ctggctggca aaagcccgtg tatgagcaga tgggtatctg
    12961 gcctggcgat gactatttct cccgtcgtta cgactatctc accgaatatg ttcaggtgct
    13021 gcgcgacctg tggggcacgg ggaaaagcga ttt
//
LOCUS       DAEEKQ010000111        12996 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.111, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000111 DAEEKQ010000000
VERSION     DAEEKQ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.111"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            96..1391
                     /locus_tag="KLK57_002936"
     CDS             96..1391
                     /locus_tag="KLK57_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HBE0893248.1"
                     /translation="MDADSSDSTVTPGRLPDGLSSCPCFYLGKKLMNLSANAQATLLL
                     TSDFSRAAASEYKPLSNSEWGKFALWLKHQRISPAELLVPQPQEKLTGWSDPRISQER
                     ILGLLARGHSLALAVDKWQRAGLWILTRGDADYPVRLKNRLRTDAPPVLFGCGNKALL
                     QAEGMAIVGSRDAPTDDLRYTQQLAAKLAQQGICVISGGARGIDECAMASALEAGGTA
                     VGVLADSLLKTSTLVKWREGLIAGNLVLISPFYPEVRFTVGNAMARNKYIYCLAESAM
                     VVRAGMTGGTITGAMEALKHQWLPVQVKPNQDMQSANSRLVENGASWSAEQAENVTIR
                     LPDVPGLMYDRALRNAQPELFSLHEDDANYAVMPAYTPVDFYQLFVAELAILAKESIS
                     IERLASCTGLTIEQISVWLNRAEEEGRVIRLGEGHYQFR"
     gene            complement(1437..2030)
                     /gene="yhgN"
                     /locus_tag="KLK57_002937"
     CDS             complement(1437..2030)
                     /gene="yhgN"
                     /locus_tag="KLK57_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HBE0893249.1"
                     /translation="MNAIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            2222..3325
                     /gene="asd"
                     /locus_tag="KLK57_002938"
     CDS             2222..3325
                     /gene="asd"
                     /locus_tag="KLK57_002938"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HBE0893250.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            3598..5784
                     /gene="glgB"
                     /locus_tag="KLK57_002939"
     CDS             3598..5784
                     /gene="glgB"
                     /locus_tag="KLK57_002939"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HBE0893251.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            5781..7754
                     /gene="glgX"
                     /locus_tag="KLK57_002940"
     CDS             5781..7754
                     /gene="glgX"
                     /locus_tag="KLK57_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HBE0893252.1"
                     /translation="MTRLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIEG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALHEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYACACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIALGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            7772..9067
                     /gene="glgC"
                     /locus_tag="KLK57_002941"
     CDS             7772..9067
                     /gene="glgC"
                     /locus_tag="KLK57_002941"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HBE0893253.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            9067..10500
                     /gene="glgA"
                     /locus_tag="KLK57_002942"
     CDS             9067..10500
                     /gene="glgA"
                     /locus_tag="KLK57_002942"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HBE0893254.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDNNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            10519..12966
                     /gene="glgP"
                     /locus_tag="KLK57_002943"
     CDS             10519..12966
                     /gene="glgP"
                     /locus_tag="KLK57_002943"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HBE0893255.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEMR
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
BASE COUNT         3040 a         3228 c         3576 g         3152 t
ORIGIN      
        1 ttccaccagt gtcaaaatct cgacggggcg tttgttatta cccctccttt gatgcctggt
       61 ccggcgttgc tggttgacga tatcgtggat tctgcatgga cgctgacagt tctgacagca
      121 ctgttacgcc aggcaggttg cccgacggtt tatcctcttg cccttgcttc tacctcggta
      181 aaaaattgat gaatctttca gccaatgcac aagcaactct cctgctaacc agcgattttt
      241 ctcgcgcggc ggcgagtgag tataaacctc ttagtaatag tgaatggggg aagtttgcat
      301 tatggctgaa gcaccaacgt atcagtcccg ccgagcttct ggtgccgcaa ccgcaagaga
      361 aacttacagg ctggagcgat ccgcgtattt ctcaggagcg tattcttggc ttgctggcgc
      421 gtggtcatag tctggcgttg gcggtagata agtggcaacg cgccggttta tggatcttaa
      481 cccgcggaga tgctgattat cccgttcgct tgaaaaaccg attgcgaacg gatgcacctc
      541 ccgttttatt tggctgcggg aataaagcat tactgcaagc ggaaggtatg gcgattgttg
      601 gctcgcgaga tgctccgact gacgatttgc gctataccca acaactggcc gcgaaactgg
      661 cccaacaggg gatttgcgtt atctctggtg gtgcgcgagg tattgatgaa tgtgcaatgg
      721 cgtcggcact ggaggccggg ggaactgccg ttggcgtatt agctgatagc ttgttaaaaa
      781 cgagtacgtt agtgaaatgg cgtgaagggc ttatagcagg caacctggtg ttgatttcgc
      841 cgttttaccc agaggtacgt ttcaccgtcg gcaatgcgat ggcgcgaaat aaatatattt
      901 attgccttgc tgaaagcgca atggttgtac gtgcgggaat gaccggtgga acgataaccg
      961 gggcgatgga ggcattaaaa catcagtggc tgcctgtgca ggttaaacca aatcaggata
     1021 tgcaatcagc caattcacga ttagtagaaa atggggcgtc atggagtgct gaacaggctg
     1081 agaatgtgac gatcagactg ccagacgttc ctgggctgat gtatgacaga gcactccgta
     1141 acgctcaacc agaactgttt tcgctgcatg aagatgacgc aaattacgca gtaatgcccg
     1201 cgtatacgcc tgtcgatttt tatcaactct ttgtggcgga actggcgatc cttgcaaagg
     1261 aatcgataag tattgaaagg ctggcgtctt gtactggttt aaccatcgaa caaattagtg
     1321 tgtggctgaa ccgcgcagaa gaagagggaa gggttatccg attgggcgaa ggtcattatc
     1381 agttcaggta ataaaacagg tgccgcgtct ggttgtgtgc agcacctgtt tgaaccttac
     1441 cccttcatcc acattcgaat gccgtcgagg aacatctggg ttgccatcat caccagaatc
     1501 aatcccatca ggcgttcaag tgcgttcacc cctttctcgc ccagcagacg taaaaatagc
     1561 gaagactgta gcaggatgac aaaggtgccg ccccaggcca gcagcagagc aatcaccaga
     1621 tgtcccatct gattcgggta ctgatgagac aacaacatca gcgtggcgag aatagtcggc
     1681 ccggcgacta acggaattgc caacggcacg ataaatggct cttcacctgc cggaagcccg
     1741 ctgctatttc ctgaagcgct ggggaaaatc atcttaatgg cgattagaaa cagaatgatg
     1801 ccgccagaaa tggagacggt ttctgctcgc aggctgagaa atgccagaat tttctcaccc
     1861 gcaaacagga acaccagcat caccaggaga gcaataagca actctcgcac catgattgcc
     1921 cgccgtcttt tcggttcagt atgtttcagt acggacatga aaataggcag gtttccgagc
     1981 ggatccataa tcaggatcaa taaaactgct gcagaaatga ttgcattcat aactcaaatt
     2041 ccctgataat tgccgcggac tttctgcgtg ctaacaaagc aggataagtc gcattactga
     2101 tggcttcgct atcattgatt aatttcactt gcgactttgg ctgctttttg tatggtgaaa
     2161 gatgtgccaa gaggagaccg gcacatttat acagcacaca tctttgcagg aaaaaacgct
     2221 tatgaaaaat gttggtttta tcggctggcg cggtatggtc ggctccgttc tcatgcaacg
     2281 catggttgaa gagcgcgact tcgacgccat tcgccctgtc ttcttttcta cttctcagct
     2341 tggccaggct gcgccgtctt ttggcggaac cactggcaca cttcaggatg cctttgatct
     2401 ggaggcgcta aaggccctcg atatcattgt gacctgtcag ggcggcgatt ataccaacga
     2461 aatctatcca aagcttcgtg aaagcggatg gcaaggttac tggattgacg cagcatcgtc
     2521 tctgcgcatg aaagatgacg ccatcatcat tcttgacccc gtcaatcagg acgtcattac
     2581 cgacggatta aataatggca tcaggacttt tgttggcggt aactgtaccg taagcctgat
     2641 gttgatgtcg ttgggtggtt tattcgccaa tgatcttgtt gattgggtgt ccgttgcaac
     2701 ctaccaggcc gcttccggcg gtggtgcgcg acatatgcgt gagttattaa cccagatggg
     2761 ccatctgtat ggccatgtgg cagatgaact cgcgaccccg tcctctgcta ttctcgatat
     2821 cgaacgcaaa gtcacaacct taacccgtag cggtgagctg ccggtggata actttggcgt
     2881 gccgctggcg ggtagcctga ttccgtggat cgacaaacag ctcgataacg gtcagagccg
     2941 cgaagagtgg aaagggcagg cggaaaccaa caagatcctc aacacatctt ccgtaattcc
     3001 ggtagatggt ttatgtgtgc gtgtcggggc attgcgctgc cacagccagg cattcactat
     3061 taaattgaaa aaagatgtgt ctattccgac cgtcgaagaa ctgctggctg cgcacaatcc
     3121 gtgggcgaaa gtcgttccga acgatcggga aatcactatg cgtgagctaa ccccagctgc
     3181 cgttaccggc acgctgacca cgccggtagg ccgcctgcgt aagctgaata tgggaccaga
     3241 gttcctgtca gcctttaccg tgggcgacca gctgctgtgg ggggccgcgg agccgctgcg
     3301 tcggatgctt cgtcaactgg cgtaatcttt attcattaaa tctggggcgc gatgccgccc
     3361 ctgttagtgc gtaatacagg agtaagcgca gatgtttcat gatttaccgg gagttaaata
     3421 gagcattggc tattctttaa gggtggctga atacatgagt attcacagcc ttacctgaag
     3481 tgaggacgac gcagagagga tgcacagagt gctgcgccgt tcaggtcaaa aaaatgtcac
     3541 aaccagaagt caaaaatcca attggatggg gtgacacaat aaaacaggaa gacaagcatg
     3601 tccgatcgta tcgatagaga cgtgattaac gcgctaattg caggccattt tgcggatcct
     3661 ttttccgtac tgggaatgca taaaaccacc gcgggactgg aagtccgtgc ccttttaccc
     3721 gacgctaccg atgtgtgggt gattgaaccg aaaaccgggc gcaaactcgc aaaactggag
     3781 tgtctcgact cacggggatt ctttagcggc gtcattccgc gacgtaagaa ttttttccgc
     3841 tatcagttgg ctgttgtctg gcatggtcag caaaacctga ttgatgatcc ttaccgtttt
     3901 ggtccgctaa tccaggaaat ggatgcctgg ctattatctg aaggtactca cctgcgcccg
     3961 tatgaaacct taggcgcgca tgcagatact atggatggcg tcacaggtac gcgtttttct
     4021 gtctgggctc caaacgcccg tcgggtctcg gtggttgggc aattcaacta ctgggacggt
     4081 cgccgtcacc cgatgcgcct gcgtaaagag agcggcattt gggaactgtt tatccctggg
     4141 gcgcataacg gtcagctcta taaatacgag atgattgatg ccaatggcaa cttgcgtctg
     4201 aagtccgacc cttatgcctt cgaagcgcaa atgcgcccgg aaaccgcgtc tcttatttgc
     4261 gggctgccgg aaaaggttgt acagactgaa gagcgcaaaa aagcgaatca gtttgatgcg
     4321 ccaatctcta tttatgaagt tcacctgggc tcctggcgtc gccacaccga taacaatttc
     4381 tggttgagct accgcgagct ggccgatcaa ctggtgcctt atgctaaatg gatgggcttt
     4441 acccacctcg aactactgcc cattaacgag catcccttcg atggcagttg gggttatcag
     4501 ccaaccggcc tgtatgcacc aacccgccgt tttggtactc gcgacgactt ccgttatttc
     4561 attgatgccg cacacgcagc gggtctgaac gtgattctcg actgggtgcc aggccacttc
     4621 ccgaccgatg actttgcgct tgccgaattt gatggcacga acttgtatga acacagcgat
     4681 ccgcgcgaag gctatcatca ggactggaat acgctgatct acaactatgg tcgccgtgaa
     4741 gtcagtaact tcctcgtcgg taacgcgctt tactggattg aacgttttgg tattgatgcg
     4801 ctgcgcgtcg atgcggtggc gtcaatgatt tatcgcgact acagccgtaa agagggggag
     4861 tggatcccga acgaatttgg cgggcgcgag aatcttgaag cgattgaatt cttgcgtaat
     4921 accaaccgta ttcttggtga gcaggtttcc ggtgcggtga caatggctga ggagtctacc
     4981 gatttccctg gcgtttctcg tccgcaggat atgggcggtc tgggcttctg gtacaagtgg
     5041 aacctcggct ggatgcatga caccctggac tacatgaagc tcgacccggt ttatcgtcag
     5101 tatcatcacg ataaactcac cttcgggatt ctctacaact acactgaaaa cttcgtcctg
     5161 ccgttgtcgc atgatgaagt ggtccacggt aaaaaatcga ttctcgaccg catgccgggc
     5221 gacgcatggc agaaattcgc taacctgcgc gcctactatg gctggatgtg ggcattcccg
     5281 ggcaagaaac tgctgttcat ggggaacgaa tttgcccaag gccgtgagtg gaaccatgac
     5341 gccagcctcg actggcatct gttggaaggc ggcgataact ggcaccacgg tgtccagcgt
     5401 ctggtgcgcg atctgaacct cacctaccgc caccataaag caatgcatga actggatttt
     5461 gacccgtacg gctttgaatg gctagtggtg gatgacaaag aacgctcggt gctgatcttt
     5521 gtgcgtcgcg ataaagaggg taacgaaatt atcgttgcca gtaactttac gccggtgccg
     5581 cgtcatgatt atcgcttcgg cattaatcag ccaggcaaat ggcgtgaaat cctcaatacc
     5641 gattccatgc actatcacgg cagtaatgca ggcaatggcg gcacggtaca cagcgatgag
     5701 attgccagcc acggtcgtca gcattcacta agcctgacac taccaccgct ggccactatc
     5761 tggctggttc gggaggcaga atgacacgac tcgccattgg caaacccgct cccctcggcg
     5821 cgcattacga cggtcagggc gtcaacttca cacttttctc cgctcatgcc gagcgggtag
     5881 agctgtgtgt ctttgacgcc aatggccagg aacatcgcta tgacttgcca gggcacagtg
     5941 gcgacatttg gcacggttat ctgccggatg cgcgcccggg tttgcgttat ggttatcgcg
     6001 ttcatggccc ctggcaaccc gccgaggggc atcgctttaa cccggcgaag ttgttgattg
     6061 atccttgcgc gcggcaaatt gaaggggaat tcaaagataa cccgctgctg cacgccggtc
     6121 ataatgaacc tgactatcgc gacaacgccg ccattgcgcc gaaatgcgta gtggtggttg
     6181 atcactatga ctgggaagat gatgccccgc cgcgcacgcc gtggggcagc accatcattt
     6241 atgaagccca tgttaaagga ttaacgtacc tgcacccgga gatcccggtc gagatccgtg
     6301 gcacttataa agccctcggg catccggtga tgatcaacta tttgaaacag ttgggcatta
     6361 ccgcgctgga actgctgccg gtggcgcagt ttgccagtga accacgtctg caacgcatgg
     6421 ggctaagtaa ctactggggt tacaacccgg tggcgatgtt tgcgctgcat ccggcgtatg
     6481 cctgctcgcc agaaacggcg ctgcatgagt ttcgcgatgc aatcaaagca ctgcataaag
     6541 cgggtatcga agtcattctt gatatcgtgc tcaaccatag tgcggaactg gacctcgacg
     6601 gcccgttatt ctcgctgcgt gggatcgaca accgtagcta ttattggata agagaagacg
     6661 gcgattatca caactggacc ggttgtggca acacgctcaa tttgagtcat ccggcggtgg
     6721 tggattatgc ctgcgcctgc ctgcgttatt gggtagaaac ctgtcacgtc gatggtttcc
     6781 gctttgatct ggcggcagtc atgggccgta cgccagagtt ccgtcaggat gcgccgttgt
     6841 ttaccgctat ccagaactgc ccggtgctct cgcaggtgaa gttaattgct gaaccgtggg
     6901 atatcgccct tggtggttat caggtgggaa atttcccgcc gctgtttgcc gagtggaacg
     6961 atcatttccg cgatgctgcc cgtcgtttct ggctacatta tgatttgcct ctgggggcgt
     7021 ttgccgggcg ttttgctgcc tccagcgatg tttttaaacg taatggtcgt ctgccgagtg
     7081 ccgcgattaa tctcgtcact gcgcatgacg gttttacgct tcgcgactgc gtttgcttca
     7141 accataaaca caatgaagca aacggagaag aaaatcgcga cgggaccaac aacaattaca
     7201 gtaacaatca tggtaaagaa gggttaggcg gttctcttga cctggttgaa cggcggcgcg
     7261 acagtattca cgccctgtta acaacgttgt tgctctccca gggtacgccg atgttactgg
     7321 ccggtgacga acacggtcac agccagcatg gcaataacaa tgcctactgt caggataacc
     7381 aattaacctg gttggactgg tcgcaggcaa gcagtggttt aaccgcattt accgccgcgt
     7441 taatccatct gcgcaagcgc attcccgctt tggtggagaa tcgctggtgg gaagaaggcg
     7501 acggcaatgt ccgttggcta aatcgatatg ctcaaccttt aagcacggat gagtggcaaa
     7561 acgggccgaa acagctgcaa attctgctct cggatcgctt tttgatcgca attaacgcca
     7621 cgcttgaggt aacagagatt gttttacctg ctggggagtg gcacgccatt cccccattcg
     7681 ctggagagga taacccagtg attacggctg tctggcaggg acctgcacac ggattgtgtg
     7741 tgttccagag atgataaaaa aggagttagt catggttagt ttagagaaga acgatcactt
     7801 aatgttggcg cgccagctgc cattgaaatc tgttgccctg atactggcgg gaggacgtgg
     7861 tacccgcctg aaggatttaa ccaataagcg agcaaaaccg gccgtacact tcggcggtaa
     7921 gttccgcatt attgactttg cgctgtctaa ctgcatcaac tctgggatcc gtcgtatggg
     7981 cgtgatcacc cagtaccagt cccacactct ggtgcagcac attcagcgcg gctggtcatt
     8041 cttcaatgaa gaaatgaacg agtttgtcga tctgctgcca gcacagcaga gaatgaaagg
     8101 ggaaaactgg tatcgcggca ccgcagatgc ggtcacccaa aacctcgaca ttatccgccg
     8161 ttataaagcg gaatacgtgg tgatcctggc gggcgaccat atctacaagc aagactactc
     8221 gcgtatgctt atcgatcacg tcgaaaaagg cgcacgttgc accgttgctt gtatgccagt
     8281 accgattgaa gaagcctccg catttggcgt tatggcggtt gatgagaacg ataaaattat
     8341 cgaattcgtt gaaaaacctg ctaacccacc gtcaatgccg aacgatccga gcaaatctct
     8401 ggcgagtatg ggtatctacg tctttgacgc cgactatctg tatgaactgc tggaagaaga
     8461 cgatcgtgat gagaactcca gccacgactt tggcaaagat ttgattccca agatcaccga
     8521 agccggtctg gcctatgcgc acccgttccc gctctcttgc gtacaatccg acccggatgc
     8581 cgagccgtac tggcgcgatg tgggtacgct ggaagcttac tggaaagcga acctcgatct
     8641 ggcctctgtg gtgccggaac tggatatgta cgatcgcaat tggccaattc gcacctacaa
     8701 tgaatcatta ccgccagcga aattcgtgca ggatcgctcc ggtagccacg ggatgaccct
     8761 taactcactg gtatccggcg gctgtgtgat ctccggttcg gtggtggtgc agtccgttct
     8821 gttctcgcgc gttcgcgtga attcattctg caacattgat tccgccgtat tgttaccgga
     8881 agtatgggta ggtcgctcgt gccgtctgcg ccgctgcgtc atcgatcgtg cttgtgttat
     8941 tccggaaggc atggtgattg gtgaaaacgc agaggaagat gcacgtcgtt tctatcgttc
     9001 agaagaaggc atcgtgctgg taacgcgcga aatgctacgg aagttagggc ataaacagga
     9061 gcgataatgc aggttttaca tgtatgttca gagatgttcc cgctgcttaa aaccggcggt
     9121 ctggctgatg ttattggggc attacccgca gcacaaatcg cagacggcgt tgacgctcgc
     9181 gtactgttgc ctgcatttcc cgatattcgc cgtggcgtga ccgatgcgca ggtagtatcc
     9241 cgtcgtgata ccttcgccgg acatatcacg ctgttgttcg gtcattacaa cggggttggc
     9301 atatacctga ttgacgcgcc gcatctctat gatcgtccgg gaagcccgta tcacgatacc
     9361 aacttatttg cctataccga caacgtattg cgttttgcgc ttctggggtg ggttggggca
     9421 gaaatggcca gcgggcttga cccattctgg cgtcctgatg tggtgcatgc gcacgactgg
     9481 catgcaggcc ttgcgcctgc gtatctggcg gcgcgcgggc gtcctgcgaa gtcggtgttt
     9541 actgtgcaca acctggccta tcaaggcatg ttttatgcac atcacatgaa tgacatccaa
     9601 ttgccatggt cattctttaa tattcatggg ctggaattca acggacaaat ctctttcctg
     9661 aaggccggtc tgtactatgc cgatcacatt acggcggtca gtccaaccta cgctcgcgag
     9721 atcaccgaac cgcagtttgc ctacggtatg gaaggtctgt tgcaacagcg tcatcgtgaa
     9781 gggcgtcttt ccggcgtact gaacggcgtg gacgagaaaa tctggagtcc agagacggac
     9841 ttactgttgg cctcgcgcta cacccgcgat acgttggaag ataaagcgga aaataagcgc
     9901 cagttacaaa tcgcaatggg gcttaaggtt gacgataaag tgccgctttt tgcagtggtg
     9961 agccgtctga ccagccagaa aggtctcgac ctggtgctgg aagccttacc gggtcttctg
    10021 gagcagggcg ggcagctggc gctactcggc gcgggcgatc cggtgctgca ggaaggtttc
    10081 cttgcggcgg cagcggaata ccccggtcag gtgggcgttc agattggcta tcacgaagca
    10141 ttttcgcatc gcattatggg cggcgcggac gtcattctgg tgcccagccg ttttgaaccg
    10201 tgcggcttaa cgcaacttta tggattgaag tacggtacgc tgccgttagt gcggcgcacc
    10261 ggtgggcttg ctgatacggt ttctgactgt tctcttgaga accttgcaga tggcgtcgcc
    10321 agtgggtttg tctttgaaga taataatgcc tggtcgctgt tacgggctat tcggcgtgct
    10381 tttgtactgt ggtcccgtcc ttcactgtgg cggtttgtgc aacgtcaggc tatggcaatg
    10441 gattttagct ggcaggtcgc ggcgaagtcg taccgtgagc tttactatcg cttgaaatag
    10501 ttttcaggaa acgcctatat gaatgctccg tttacatatt catcgcccac gcttagcgta
    10561 gaagctctta agcactctat cgcttacaag ctgatgttta cgattggaaa ggacccggtc
    10621 gtcgccaata aacatgaatg gctgaacgca acgttatttg ctgtgcgcga tcgtctcgtg
    10681 gagcgctggt tacgttcaaa ccgtgcccag ttgtcgcaag aaacacgtca ggtttactac
    10741 ctgtcgatgg agtttttgat tggccgtacg ctctccaacg ccatgttgtc gctaggaatt
    10801 tacgaagatg tacagggcgc actggaagcg atggggttaa atctcgaaga gctgattgat
    10861 gaagaaaatg acccaggcct cggtaacggt ggcctgggac gtctggcggc ttgcttcctt
    10921 gattctctgg cgacgttagg gttgccgggg cgcggttacg gcatccgcta tgactacggt
    10981 atgttcaagc agaacatcgt taacggtagc cagaaagagt cgccagacta ctggctggaa
    11041 tacggtaacc cgtgggaatt caaacgccac aacacgcgct ataaagtccg ttttggcggt
    11101 cgcattcagc aggaaggtaa aaaaacgcgc tggattgaaa ccgaagagat tctgggagtc
    11161 gcttacgatc agataatccc tggttacgac accgacgcga ccaacacgct gcgtttgtgg
    11221 agtgcgcaag ccagtagcga aattaacctc ggtaaattca accagggtga ctacttcgcg
    11281 gcagtggaag ataaaaacca ctccgagaac gtatctcgcg tactgtatcc ggatgactcc
    11341 acctactccg ggcgtgagct gcgcctgcgt caggaatact tcctggtttc ctcgaccatt
    11401 caggacattt taagccgcca ttatcagttg cataaaacct acgataacct ggcggataaa
    11461 atcgcgattc atctcaatga tacccatccg gtactgtcga ttcctgagat gatgcgtctg
    11521 ctgatcgatg agcaccaatt tagctgggac gacgcgtttg aggtgtgttg tcaggtcttc
    11581 tcctacacta accacacgct gatgagcgag gcgctggaaa cctggccggt tgatatgctg
    11641 ggtaaaattc tgccgcgtca cctgcagatc atctttgaaa tcaacgacta tttcctgaaa
    11701 accttgcagg aacagtatcc gaacgatacc gatctgctgg gacgggcgtc gatcattgat
    11761 gaatccaacg gtcgtcgtgt gcgtatggcc tggctggcgg ttgttgtgag ccacaaagtt
    11821 aacggtgtat cggaactgca ctctaatctg atggtgcaat cgttgtttgc cgactttgcg
    11881 aaaatcttcc cgggtcgttt caccaacgtc accaacggtg tgacgccgcg tcgctggctg
    11941 gcggtagcga acccatcgct ttcagccgtg ctggacgaac acctgggccg taactggcgc
    12001 accgacctta gcctgcttaa tgagctgcaa caacactgtg atttcccaat ggttaatcac
    12061 gctgtgcatc aggcgaagct ggagaacaaa aagcgtctgg cagagtatat cgcccagcag
    12121 ctgaatgtgg tggtgaatcc aaaggcgttg ttcgatgtac aaatcaaacg tattcacgaa
    12181 tacaaacgtc aattgatgaa tgtgttgcat gtgattaccc gctataaccg catcaaggcc
    12241 gacccggatg cgaagtgggt accgcgcgtg aatatttttg gcggtaaggc ggcttcggcc
    12301 tattacatgg cgaagcacat tattcatttg atcaatgacg tagcgaaagt gatcaacaac
    12361 gatccgcaga ttggcgataa gctgaaagtc gtgttcatcc cgaactacag cgttagcctg
    12421 gcgcagttga tcattccggc ggcagatctg tctgaacaga tttcgctggc agggacggaa
    12481 gcttccggca ccagtaacat gaagtttgcg cttaacggtg cgctgactat cggtacgttg
    12541 gacggtgcga atgtcgagat gcgggatcat gtcggtgctg acaatatctt tatttttggt
    12601 aacacagcgg aagaagtgga agaactgcgt cgtcagggct acaaaccgcg tgaatactac
    12661 gagaaagatg aggagctgca tcaggtgctg acgcaaatcg gcagcggtgt attcagtccg
    12721 gaagatccgg gtcgctatcg cgatctggtt gattcgctga tcaacttcgg cgatcactat
    12781 caggttctgg cggattatcg cagctatgtc gattgtcagg ataaagtcga cgaactctac
    12841 gagcttcagg aagagtggac cgcaaaagcg atgctgaaca ttgccaatat gggctacttc
    12901 tcttctgacc gtactatcaa agagtacgcc gatcatatct ggcatatcga tccggtgaga
    12961 ttgtaagttc accaataaat agaacggggc caaagg
//
LOCUS       DAEEKQ010000112        12940 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.112, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000112 DAEEKQ010000000
VERSION     DAEEKQ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.112"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(314..1201)
                     /gene="rarD"
                     /locus_tag="KLK57_002944"
     CDS             complement(314..1201)
                     /gene="rarD"
                     /locus_tag="KLK57_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HBE0893256.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1253..1720)
                     /locus_tag="KLK57_002945"
     CDS             complement(1253..1720)
                     /locus_tag="KLK57_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HBE0893257.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1885..2754
                     /gene="pldA"
                     /locus_tag="KLK57_002946"
     CDS             1885..2754
                     /gene="pldA"
                     /locus_tag="KLK57_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HBE0893258.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYCFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2887..4716
                     /gene="recQ"
                     /locus_tag="KLK57_002947"
     CDS             2887..4716
                     /gene="recQ"
                     /locus_tag="KLK57_002947"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBE0893259.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4780..5397
                     /gene="rhtC"
                     /locus_tag="KLK57_002948"
     CDS             4780..5397
                     /gene="rhtC"
                     /locus_tag="KLK57_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HBE0893260.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLMVGGGLYLCWMGYQMLRGALKKEAVSAPA
                     PQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVETL
                     AWFIVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5459..6079)
                     /gene="rhtB"
                     /locus_tag="KLK57_002949"
     CDS             complement(5459..6079)
                     /gene="rhtB"
                     /locus_tag="KLK57_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HBE0893261.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6190..7212
                     /gene="pldB"
                     /locus_tag="KLK57_002950"
     CDS             6190..7212
                     /gene="pldB"
                     /locus_tag="KLK57_002950"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HBE0893262.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7220..8020
                     /gene="yigL"
                     /locus_tag="KLK57_002951"
     CDS             7220..8020
                     /gene="yigL"
                     /locus_tag="KLK57_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HBE0893263.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNLDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8096..8995
                     /gene="bioP"
                     /locus_tag="KLK57_002952"
     CDS             8096..8995
                     /gene="bioP"
                     /locus_tag="KLK57_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HBE0893264.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8883..9836)
                     /gene="metR"
                     /locus_tag="KLK57_002953"
     CDS             complement(8883..9836)
                     /gene="metR"
                     /locus_tag="KLK57_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HBE0893265.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     ITEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            10072..12333
                     /gene="metE"
                     /locus_tag="KLK57_002954"
     CDS             10072..12333
                     /gene="metE"
                     /locus_tag="KLK57_002954"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HBE0893266.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(12618..12863)
                     /locus_tag="KLK57_002955"
     CDS             complement(12618..12863)
                     /locus_tag="KLK57_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HBE0893267.1"
                     /translation="MITETEINVLKIMAEKDINWNWMNLDRTLSIRQIPGFGNVVNIV
                     NKLANEGLVIIEDSGHKSMPHYHVTEKGYNFVKSENK"
BASE COUNT         3053 a         3241 c         3458 g         3188 t
ORIGIN      
        1 ggtaaagaac cttacagcaa aaaatctgat tttgttaagg catggttatt gtggtgggct
       61 attaccatcc ttgttctatt tattttaagc ctgactacga tattaaattt aagaagtata
      121 ggtatagggt taagttttgg gttttgtcaa atccttggta gatatacgat gtctctagga
      181 tggagtaagt actggggagt tttgggggta tttcctgtaa caaatattgt tctttttctt
      241 atcttaattt ttttcaaaaa aagataaata agtcagtggg taggctcttt aaacggccta
      301 cccatgttaa ttcttaactt tttctacgct gcgtataaat cgcatccatc acaaaaattg
      361 ccagcgccac ccaaataaag gcgaaagtca ccatcttatc ggcacccggt ttttcaccat
      421 aaaacgtcac agccagcagg aacatcagcg tcgggccaat gtactggaaa aagcctaacg
      481 ttgagagacg caagcgcgtg gcagcggcgg taaaacacaa cagcggtacg gtagtgacaa
      541 taccggcggc gatcagcagt aaattcagcg acatcgggtt ttgccccata tggctggttg
      601 agctgtcggc aatagcaaac aggtaaattg ccgccacggg cagcagccac atggtttcga
      661 ttaacatgcc ggtttgcgct tcaacggcaa tcttcttgcg taccagaccg tagaaggcaa
      721 aactaaatgc cagtcccagc gcgataatag gtagcgaacc aaaagtccac agctggacta
      781 acacgccaca tatcgccaga atcaccgcca gccattgcat ccggcggaat cgctcgccga
      841 ggaaaatcat ccccagcaca atgttcacca gcgggttaat aaagtaacca aggctcgctt
      901 ccagcatatg gtgattgttc accgcccaga taaacagtag ccagttgcca ccaatcagca
      961 cggcagagac tgccagcata aaaattttct gtggcgtctg aatcagcgtt tttaaatagg
     1021 accactggcg gcaaatgctc atcagcacca ccataaagaa aaacgaccag atcacgcgat
     1081 gcgtcaggat ttcatcggcg ggcacgtagt aaatcaactt gaagtacgct ggcgctatac
     1141 cccaaataaa ataagcggca agagcgagta atacgccctg ccgcgtttgt tttgcatcca
     1201 tcgggaatac tcatttttaa ttggtaacag cagtttacct gcttttatgt cttcaaccta
     1261 ccatataggt ggcggtggca ctggcaatat aaagctgttc ttcattgtgt aattcaacgc
     1321 gggcgacggc gactttattg cctgcacgca acaggctact ggtagcagta aaacgctcgc
     1381 ccctgcctgg gcgcagataa tcaacgcgaa gatcaatggt ccccatccgc gatagccgct
     1441 ggcgtagttc atcttcactg atggtttcgt ggcgggttaa ggtacttccc acgcacacca
     1501 gaccggcggc gacatccagc gccgacgcaa tgaccccgcc gtgcaaaatg ctttgcgccc
     1561 agttgcccac catcattggc tgatttttaa aggccagctg tgcgaactct ttttcgtaac
     1621 gctccagttc catccccaat gcgcggttaa atggcatgtg ataaacaaac atctcaccca
     1681 ctaatttcag ggcttgttca gcggtcagta cggcagacat atcatcctta cacttcattg
     1741 gttaatgaaa tgttgatttt atgcttcttt gttgttggtt tctactttag gaagggataa
     1801 ctaacggcta tggagttaag tatgtaaaat agcccgcaga aaaatattca ccttatcaat
     1861 aattcgttac ggagaacacg accgatgcgg actctgcagg gctggttgtt gccggtgttt
     1921 atgttgccta tggcagtata tgcacaagag gcaacggtga aagaggtgca tgacgcgcca
     1981 gcggtgcgtg gcagtattat cgccaatatg ctgcaggagc atgacaatcc gttcacgctc
     2041 tatccttatg acaccaacta cctcatttac acccaaacca gcgatctgaa taaagaagcg
     2101 attgccagtt acgactgggc ggaaaatgcg cgtaaggatg aagtaaagtt tcagttgagc
     2161 ctggcatttc cgctgtggcg tgggatttta ggcccgaact cggtgttggg tgcgtcttat
     2221 acgcaaaaat cctggtggca actgtccaat agcgaagagt cttcaccgtt tcgtgaaacc
     2281 aactacgaac cgcaattgtt cctcggtttt gccaccgatt actgttttgc aggttggacg
     2341 ctgcgcgatg tggagatggg gtataaccac gactctaacg ggcgttccga cccgacctcc
     2401 cgcagctgga accgccttta tactcgcctg atggcagaaa acggtaactg gctggtagaa
     2461 gtgaagccgt ggtatgtggt gggtaatact gacgataacc cggatatcac caaatatatg
     2521 ggttactacc agcttaaaat cggctatcac ctcggtgatg cggtgttgag tgcgaaagga
     2581 cagtacaact ggaataccgg ttacggcggc gcggagttag gcttaagtta cccgatcacc
     2641 aaacatgtgc gcctttatac tcaggtttac agcggctatg gcgaatcgct catcgactat
     2701 aacttcaacc agacccgtgt cggtgtagga gttatgctaa acgacttgtt ttgatgaacg
     2761 gttgagtggt tggcaaatct ggaacccagc atccaggatt accctctcag agactaaaag
     2821 cattgcagtt tctcgcgcag gcgctgaaaa tagcgcctgt ttttatttca ggcaatcggg
     2881 gggaatgtgg cgcaggcgga agtgttgaat ctggagtccg gagctaaaca ggttttacaa
     2941 gaaacctttg gctaccaaca gtttcgcccc ggccaggaag aaattatcga cactgtgctt
     3001 tccggtcgcg attgcctggt cgtcatgccc accggtggcg gaaaatccct ttgctatcaa
     3061 attcctgcct tattgctaaa cggccttacc gtggttgttt caccgctgat ttcgttgatg
     3121 aaagatcagg tggatcaact gcaagccaac ggcgtggcgg cggcgtgcct taactcgacg
     3181 caaacccgcg agcagcaact tgaagtgatg acaggctgcc gcaccgggca aattcgtctg
     3241 ctttatatcg ccccggaacg cctgatgctg gataactttc ttgagcatct ggcgcactgg
     3301 aatccggtgt tattagccgt tgatgaagcg cactgtatct cccaatgggg ccacgatttc
     3361 cgcccggaat atgccgcgct tggtcagtta cgccagcgtt tcccgaccct gccgtttatg
     3421 gcgctgactg ccacagccga cgacaccacg cgccaggata tcgtgcgctt gctggggctg
     3481 aacgatccgc tgattcaaat cagcagtttt gaccgtccga atattcgcta catgctgatg
     3541 gagaaattca aaccgctcga tcagttgatg cgctacgtgc aggaacagcg cggtaagtcc
     3601 ggcattatct actgcaacag ccgggcgaaa gtagaagaca ccgctgcgcg cctgcaaagc
     3661 aagggtatta gcgcggcggc ctatcatgcc gggctggaaa ataatgtccg cgccgacgtg
     3721 caggagaaat tccagcgcga tgacctgcaa attgtggtgg cgacggtggc gttcggcatg
     3781 ggcatcaata aaccaaacgt tcgcttcgtg gtccactttg atattccgcg caatatcgaa
     3841 tcctattatc aggaaaccgg tcgcgccggg cgtgatggcc tgcctgcgga agcgatgctg
     3901 ttttacgatc cggctgatat ggcgtggctg cgccgttgtc tggaagagaa gccgcagggg
     3961 cagttgcagg atattgagcg ccacaaactc aatgcgatgg gcgcgtttgc cgaagcgcaa
     4021 acttgccgtc gtctggtatt gctgaactat tttggcgaag ggcgtcagga gccgtgcggg
     4081 aactgcgata tctgcctcga tccgccgaaa cagtacgacg gttcaaccga tgctcagatt
     4141 gccctttcca ccattggtcg tgtgaatcag cggtttggga tgggttatgt ggtggaagtg
     4201 attcgtggtg ctaataacca gcgtatccgc gactatggtc atgacaaact gaaagtctat
     4261 ggcatgggcc gtgataaaag ccatgaacat tgggtgagcg tgatccgcca gctgattcac
     4321 ctcggcctgg tgacgcaaaa tattgcccag cattctgccc tacaactgac agaggccgcg
     4381 cgcccggtgc tgcgcggcga atcctctttg caacttgccg tgccgcgtat cgtggcgctc
     4441 aaaccgaaag cgatgcagaa atcgttcggc ggcaactatg atcgtaaact gttcgccaaa
     4501 ttacgcaaac tgcgtaaatc gattgccgat gaaagtaatg tcccgccgta cgtggtgttt
     4561 aacgacgcaa ccttgattga gatggctgaa cagatgccga tcaccgccag cgaaatgctc
     4621 agcgttaacg gcgttgggat gcgcaagctg gaacgctttg gtaaaccgtt tatggcgctg
     4681 attcgtgcgc atgtcgatgg cgacgacgaa gagtagtcag cagcataaaa aagtgccagg
     4741 atgaagactc cgtaaacgtt tcccccgcga gtcaaatgta tgttgatgtt atttctcacc
     4801 gtcgccatgg tgcacattgt ggcgcttatg agccccggtc ccgatttctt ttttgtctct
     4861 cagaccgctg tcagtcgttc ccgtaaagaa gcgatgatgg gcgtgctggg cattacctgc
     4921 ggtgtaatgg tttgggctgg gattgcgttg cttggcctgc atttgattat cgaaaaaatg
     4981 gcctggctgc atacgcttat ggttggcggt ggcctgtatc tctgctggat gggttaccag
     5041 atgctgcgcg gcgcgctgaa aaaagaggcg gtttctgcac ctgcgccaca ggtcgagctg
     5101 gcgaaaagtg gacgcagttt cctgaaaggt ttactgacca atctcgctaa tccgaaagcg
     5161 attatctact ttggctcggt gttctcattg tttgtcggtg ataacgttgg cactaccgcg
     5221 cgctggggca tttttgcgct gatcattgtc gaaacgctgg cgtggtttat cgtcgttgcc
     5281 agcctgtttg ccctgccgca aatgcgccgt ggttatcaac gtctggcgaa gtggattgat
     5341 ggttttgccg gggcgttatt tgccggattt ggcattcatt tgattatttc gcggtgatgc
     5401 cagacgcgtc ttcagagtaa gtcggataag gcgtttacgc cgcatccgac attatttttc
     5461 acgcatgcct cgccgatgct aacagcgctc ccaccagcat aaacaacgag ccgaaaatct
     5521 tattcagcgc cttcatctgc tttggtcctt taatccatag agcaatccgt tgagcaaggg
     5581 tggcgtaacc gatcatcaca ataatatcga ccacaatagt ggtgacgccg agcacgatat
     5641 actgcatcag ttgcggctgt tgcggcatga tgaattgcgg aaatagcgcc gccagaaaca
     5701 caatactttt gggattggtg agattcacaa aaactgcgcg ctggaacaaa tgtcgacgcg
     5761 attgagtaga ggccagcgat ttaaggtcaa ttgcaccagc ggcgcgccac tgctggattc
     5821 ccagccaaat caagtaagcc gcgcctgccc acttcaacac ttcaaacgca atcactgagc
     5881 gggaaaatag cgtccccaac cccacgccaa ccagcacaat atgaatcgcc agtccggtct
     5941 gaagcccagc aatagacgcc accgcgccgc gataaccgtg gttgagcgag gtggtcatag
     6001 tgttgattgc accagagcct ggcgacaggc ttaaaatgat cgatgtcagc aggtaggcaa
     6061 accaccattc taaggtcatg atgaactccc ggtgtgtcta tttttgtgcc acaatacgct
     6121 actgtcgcag cgttgtgtca ggcacgctaa aaaaaacgat tttacgtggt ttaagaggca
     6181 gattacccga tgtttcagca gcaaaaagac tgggaaacaa gagaaaacgc gtttgctgct
     6241 tttaccatgg gaccgctgac tgatttctgg cgtcagcgtg atgaagcaga gtttactggt
     6301 gtggatgaca ttccggtgcg ctttgtccgt tttcgcgcac agcaccatga ccgggtggta
     6361 gtcatctgcc cggggcgtat tgagagctac gtaaaatatg cggaactggc ctatgacctg
     6421 ttccatttgg ggtttgatgt cttaatcatc gaccatcgcg ggcagggacg ttccggtcgc
     6481 ctgttagccg atccgcatct cgggcatgtt aatcgcttta atgattatgt tgatgatctg
     6541 gcggcattct ggcagcagga ggttcagccc ggtccgtggc gtaaacgcta tatactggca
     6601 cattcgatgg gcggtgcgat ctccacatta tttctgcaac gccatccagg tgtatgtgac
     6661 gccattgcgc taactgcgcc aatgtttggg atcgtgattc gtatgccgtc atttatggca
     6721 cggcagatcc tcaactgggc cgaagcgcat ccacgtttcc gtgatggcta tgcaataggc
     6781 accgggcgct ggcgcgcgtt gccgtttgct atcaacgtac tgacccacag cagacagcga
     6841 tatcgacgta acttacgctt ctatgctgat gacccaacga ttcgcgtcgg tgggccgacc
     6901 taccattggg tacgcgaaag tattctggct ggcgaacagg tgttagccgg tgcgggtgat
     6961 gacgccacgc caacgcttct cttgcaggct gaagaggaac gcgtggtgga taaccgcatg
     7021 catgaccgtt tttgtgaact ccgcaccgcc gcgggccatc ctgtcgaagg aggacgaccg
     7081 ttggtaatta aaggtgctta ccatgagatc ctttttgaaa aggacgcaat gcgctcagtc
     7141 gcgctccacg ccattgttga ttttttcaac aggcataact cacccagcgg aaaccgctct
     7201 acagaggttt aaatttctta tgtaccaggt tgttgcgtct gatttagatg gcacgttact
     7261 ttctcccgac catacgttat ccccttacgc caaagaaacc ctgaagctgc tcaccgcgcg
     7321 cggcatcaac tttgtgtttg cgaccggtcg tcaccacgtt gatgtggggc aaattcgcga
     7381 taatctggag atcaagtcgt acatgatcac ctccaacggc gcgcgcgttc acgatctgga
     7441 tggcaatctg atttttgctc ataacctgga tcgcgacatt gccagcgatc tgtttggcgt
     7501 agtcaacgac aatctggaca tcattactaa cgtttatcgc gacgacgaat ggtttatgaa
     7561 tcgccatcgc ccggaagaga tgcgcttttt taaagaagcg gtgttcaaat atgcgctgta
     7621 tgagcctgga ttactggagc cggaaggcgt cagcaaagtg ttcttcacct gcgattccca
     7681 tgaacaactg ctgccgctgg agcaggcgat taacgctcgt tggggcgatc gcgtcaacgt
     7741 tagtttctct accttaacct gtctggaagt gatggcgggc ggcgtttcaa aaggccatgc
     7801 gctggaagcg gtggcgaaga aactgggcta cagcctgaag gattgtattg cgtttggtga
     7861 cgggatgaac gatgccgaaa tgctgtcgat ggcggggaaa ggctgcatta tgggcagtgc
     7921 gcaccagcgt ctgaaagacc ttcatcccga gctggaagtg attggtacta atgccgacga
     7981 cgcggtgccg cattatctgc gtaaactcta tttatcgtaa tcgttcttta tttggtcagt
     8041 tgtcaacctg atacttcgct acaatggata ctcgttaatc aaagagtttt ccattgtggc
     8101 gctacttatc atcaccacga ttctgtgggc cttctccttt agcttttatg gcgagtacct
     8161 tgcggggcac gtcgatagct attttgcggt gctggtgcgc gttggcctgg cggcactcgt
     8221 ttttctgccg tttctgcgta cccgtggcaa tagcctgaaa acggtcggcc tgtatatgct
     8281 ggtgggcgcg atgcagctcg gcgtgatgta tatgctgagt ttccgcgctt atctctacct
     8341 gacggtctcc gaattgttgc tgttcaccgt actgacgccg ctctacatca cgctgattta
     8401 tgacatcatg agtaagcgcc gtctgcgctg gggctatgct tttagcgcct tgctggcggt
     8461 gattggtgcc gggattattc gctatgatca ggttaccgac catttctgga ctggcttgtt
     8521 gctggtgcaa ctctccaata tcacttttgc catcggcatg gtgggttaca aacgcctgat
     8581 ggaaactcgc ccgatgccgc agcataacgc ctttgcgtgg ttctatcttg gcgcgttcct
     8641 ggtggcggta attgcatggt tcttgctggg aaatgcgcag aaaatgccgc aaaccacgtt
     8701 gcaatggggc attctggtgt ttcttggcgt ggtggcttcc gggattggct actttatgtg
     8761 gaactacggc gcgacgcagg tggacgccgg aacgctgggc attatgaata atatgcacgt
     8821 tccggcaggg ctgctggtaa acctggctat ctggcaccaa cagccgcact ggccaacgtt
     8881 cattacaggc gcgctggtga tcctggcctc actgtgggtg catcgtaagt gggtcgctcc
     8941 gcgctcttca caaacggcag atgatcgcag gcgtgattgc gcgctgagcg aataaatgct
     9001 tctgtaatcg gctgacgctg ctcgccatcg cgcacggcgg cgtacagtcg gctccacaag
     9061 ccttcgccca gggttttggt caccaccaga ccttggcgct caaaactctc tactacccaa
     9121 tgcggtagcg cggcaatacc catccgcgcg gcaaccatct gaatcaacaa taaggtgtta
     9181 tcgacgcttt tcagtgacgg gctgacgcct gccggctgaa gaaaatgccg ccagacatcc
     9241 agtcgactac gctgcaccgg ataaatcaac agcgtctcgc tggcgagatc ttccggtgta
     9301 attcgcgttt tcgccgccag tggatggtca ggtgctaaca ccagacgcac ttcatagtcg
     9361 aacatcggcg aataatgcag gccactgcgc ggcaggatat cggaggtcat caccagatcc
     9421 agctcgccct gttgcaacgc aggctgcggg tcaaacgtca cgcccgattt aaaatccatc
     9481 tctacctgcg gccagttctt atggaaattt tctaacgcgg gcgtcagcca ctgaatacag
     9541 ctatggcact caatggcaat gcgcagacgc gtctgctgcg gttcattgca ggcttgcagt
     9601 gcctggctga tttgcggcag cacctggttt gccagttgca acaggatttc tccctgcggt
     9661 gtaaagcgta gcggctggct cttacgcaca aatagccgga agccaaggcg ttgttccaga
     9721 tcgctaaact ggtgagacag ggcggattgc gtctgatgca acgtcgccgc agcggctgcg
     9781 agagagccgc agttccgcaa cgcttgtagc gttttcaggt gttttacttc gatcatgaaa
     9841 gtccttcact tcggcatgaa taatttgcgc ttgaggaata tacagtaacc gccaattatg
     9901 gatgtgtaaa catctggacg gctaaaatcc ttcgtctttt aaatttatgg tgcgttggct
     9961 gcgtttctcc accccggtca cttacttcag taagctcccg gggatgaata aacttgccgc
    10021 cttccctaaa ttcaaaatcc ataggattta catataatta gaggaagaaa aatgacaata
    10081 ttgaatcaca ccctcggttt ccctcgcgtt ggcctgcgtc gcgagctgaa aaaagcgcaa
    10141 gaaagttatt gggcggggaa ctccacgcgt gaagaactgc tgacagtagg gcgtgaactg
    10201 cgtgctcgtc actgggatca acaaaagcaa gcgggtatcg acctgctgcc ggtgggcgat
    10261 tttgcctggt acgatcatgt actgaccacc agtttgctgc tgggtaatgt tccgccacgt
    10321 catcagaaca aagacggttc ggtagatatc gacaccctgt tccgtattgg tcgtggacgt
    10381 gcgccgactg gcgaacctgc ggcggcagcg gaaatgacca aatggtttaa caccaactat
    10441 cactacatgg tgccggagtt cgttaaaggc caacagttca aactgacctg gacgcagctg
    10501 ctggaggaag tggacgaggc gctggcgctg ggccacaacg ttaagcctgt gctgctgggg
    10561 ccggttactt acctgtggct ggggaaagtg aaaggtgaac agtttgaccg ccttagcctg
    10621 ctgaacgaca ttctgccggt ttatcagcaa gtgctggcag aactggcgaa acgcggcatc
    10681 gagtgggtac agattgatga acccgcgctg gtactggaac taccacaggc gtggctggac
    10741 gcatacaaac ccgcttacga cgcgctccag ggacaggtga aactgctgct gaccacctat
    10801 tttgaaggcg taacgccaaa tctcgacacg attactgcgc tgcctgttca gggtctgcat
    10861 gttgatctcg tacatggtaa agatgacgtt gctgaactgc acaagcgcct gccttctgac
    10921 tggttgctgt ctgcgggtct gatcaatggt cgtaacgtct ggcgcgccga tcttaccgag
    10981 aaatatgcgc aaattaagga cattgtcggc aaacgtgatt tgtgggtggc atcttcctgc
    11041 tcgttgctgc acagccccat cgacctgagc gtggaaacgc gtcttgatgc agaagtgaaa
    11101 agctggtttg ccttcgccct acaaaaatgc catgaactag cactgctgcg cgatgcgctg
    11161 aacagtggtg acacggcagc tctggcagag tggagcgccc caattcaggc gcgtcgtcac
    11221 tctactcgtg tacataatcc ggcggtagaa aagcgtctgg cggcgatcac cgcccaggac
    11281 agccagcgtg cgaatgtcta tgaagtgcgt gctgaagccc agcgtgcacg ttttaaactg
    11341 ccagcgtggc cgaccaccac gattggttcc ttcccgcaaa ccacggaaat tcgtaccctg
    11401 cgtctggatt tcaaaaaggg caatctcgac gccaataact accgcacggg cattgcggaa
    11461 catatcaggc aggccattgt tgaacaggaa cgtttgggac tggatgtgct ggtacatggc
    11521 gaggccgagc gtaatgacat ggtggaatac tttggcgagc acctcgacgg gtttgtcttt
    11581 acgcaaaacg gttgggtaca gagctacggt tcccgctgcg tgaagccacc gattgttatt
    11641 ggtgacgtta gtcgcccggc accgattacc gtggagtggg cgaagtatgc gcaatctctg
    11701 actgataaac cggtgaaagg gatgctgact ggcccggtga ctattctctg ctggtcgttc
    11761 ccgcgtgaag atgtcagccg tgaaaccatc gccaaacaga ttgcgctggc gctgcgtgat
    11821 gaagtggccg atctggaagc cgctggaatt ggcatcatcc agattgacga accggctctg
    11881 cgcgaaggtt taccgctgcg tcgtagcgac tgggatgcgt atctccagtg gggcgtagag
    11941 gccttccgta tcaacgccgc cgtggcgaaa gatgacacac aaatccacac tcacatgtgt
    12001 tattgcgagt tcaacgacat catggattcg attgcggcac tggacgcaga cgtcatcacc
    12061 atcgaaacct cgcgttctga catggagttg ctggagtcgt tcgaagagtt tgattatccg
    12121 aatgaaatcg gtccaggcgt ctatgacatt cactcgccaa acgtaccgag cgtggaatgg
    12181 attgaagcct tgctgaagaa agcggcaaaa cgcattccgg cagagcgcct gtgggttaac
    12241 ccggactgtg gcctgaaaac gcgcggctgg ccagaaaccc gcgcggcact ggcgaacatg
    12301 gtgcaggcgg cgcagaattt gcgtcgggga tgatgaatat taacgagtca ctgtaaattg
    12361 cagtacgatg aaagaggcaa aactgcctga tactaggcct acataattta tgcaatttat
    12421 tgaatttttg ggttttgttg gccggacaag gcgttcacgc cgcatccggc atgaacaacg
    12481 cgcactttgc caataatctg atggcttcca tcaccggaag ccattttgct atctgcgccc
    12541 gttcagatac agaaataacc gaatcatttt tacgcaaaat ataatcctga gcgatttgtt
    12601 cagtaatcaa cgctacgtta tttattctca cttttcacga aattgtatcc cttttcagta
    12661 acgtggtagt gaggcataga cttatgtccg ctatcctcaa taataaccaa accctcgtta
    12721 gctaatttgt taacaatgtt aacaacatta ccgaatcctg gaatttgcct tatggatagt
    12781 gtgcgatcga gattcatcca gttccagttt atatctttct cagccataat tttcagtacg
    12841 ttaatttcag tttcagttat catagtcaag tgcatcctgg tggtataagt cattaagttc
    12901 tcccttaagt ttataatctt ctagagtagc agtatgcgta
//
LOCUS       DAEEKQ010000113        12487 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.113, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000113 DAEEKQ010000000
VERSION     DAEEKQ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.113"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            210..422
                     /gene="cspG"
                     /locus_tag="KLK57_002956"
     CDS             210..422
                     /gene="cspG"
                     /locus_tag="KLK57_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HBE0893268.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            433..621
                     /locus_tag="KLK57_002957"
     CDS             433..621
                     /locus_tag="KLK57_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HBE0893269.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            596..826
                     /gene="ymcE"
                     /locus_tag="KLK57_002958"
     CDS             596..826
                     /gene="ymcE"
                     /locus_tag="KLK57_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HBE0893270.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLLRPEEVNSEY"
     gene            816..989
                     /locus_tag="KLK57_002959"
     CDS             816..989
                     /locus_tag="KLK57_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HBE0893271.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(1038..2111)
                     /locus_tag="KLK57_002960"
                     /pseudo
     CDS             complement(1038..2111)
                     /locus_tag="KLK57_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
     gene            complement(2183..4927)
                     /gene="torS"
                     /locus_tag="KLK57_002961"
     CDS             complement(2183..4927)
                     /gene="torS"
                     /locus_tag="KLK57_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HBE0893272.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGRMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGRRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NATTSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHQEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     QLMGAFRSSVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCLSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            5010..6038
                     /gene="torT"
                     /locus_tag="KLK57_002962"
     CDS             5010..6038
                     /gene="torT"
                     /locus_tag="KLK57_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HBE0893273.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASMPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(6011..6703)
                     /gene="torR"
                     /locus_tag="KLK57_002963"
     CDS             complement(6011..6703)
                     /gene="torR"
                     /locus_tag="KLK57_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HBE0893274.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6823..7995
                     /gene="torC"
                     /locus_tag="KLK57_002964"
     CDS             6823..7995
                     /gene="torC"
                     /locus_tag="KLK57_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HBE0893275.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKDEK"
     gene            7995..10406
                     /gene="torA"
                     /locus_tag="KLK57_002965"
                     /pseudo
     CDS             7995..10406
                     /gene="torA"
                     /locus_tag="KLK57_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="KLK57_002966"
     CDS             complement(11045..11350)
                     /gene="cbpM"
                     /locus_tag="KLK57_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HBE0893276.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="KLK57_002967"
     CDS             complement(11350..12270)
                     /gene="cbpA"
                     /locus_tag="KLK57_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HBE0893277.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNHQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT         3011 a         3204 c         3269 g         3003 t
ORIGIN      
        1 ggacggctaa aataaaattt gcttaatctc aattatcatg cgttaatagc tgcgtcggtt
       61 tgaaagacag acagcataca aagtagttta ctaaagcagt tctcattatc aggcattatc
      121 cccttctttt gagtctctct cctgaacact aagtagtttc tgtattaaag ccctgtttgc
      181 cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa aatgactggt ttagtaaaat
      241 ggtttaacgc agataaaggt tttggcttta tcactcctga tgatggcagc aaagacgttt
      301 tcgtccattt caccgccatc cagagcaatg aattccgcac gctgaacgaa aatcagaaag
      361 ttgaattttc tattgagcag gggcaacgtg gccccgcggc agcgaacgtt gttacgctct
      421 aaggttgccg ttattactca acatctccat ttccgctgtc catgttgtca tggttcacag
      481 taccgcacat cggcattcga tgtgacggag cgaaaccctt tgggcgctaa gtgtattttt
      541 tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc aaatacgcac tgaccatgcg
      601 ccgctggatt tcacaaaata atatcaggct ccctcgtgga gcctttttta tatctgcctt
      661 atttttcttc aacgctgtat gtatagtaag cgataacctg ttgattattg aatctttcgg
      721 ggagatggct tataacattt cttacctgac cagggtaccg ggaaccaaca ccttactggc
      781 gtgttgctgt cttttaagac cagaagaggt taacagtgaa tattgaagag ttaaaaaaac
      841 aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat cgccgagctg aacaagaata
      901 cagggaaaga agtctctgaa attcgcttca ccgcacgaga aaaaatgacc gggcttgaaa
      961 gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca tcaccccgtg cgaggtgatg
     1021 ttccgcttgt tgctaattta gtgaccaatc attggcgctt gtggaattaa gcgtcggtac
     1081 aattcctccg gcaccgggct ttgccatact cccgcataca ttgcgtaacc aatcaccgcg
     1141 aacataatcc tcagaaccag tagcgtcatt aaccagccag acaacgcaaa agctttttta
     1201 tttgccgcag gtttttgcag tgaaaaggtc aatgttgagg ctaccggaca tgactctacg
     1261 caagtcatac agccggtaca ttccactgtt cgtacctgaa ttaatttatc taccgggatc
     1321 cgtgatgggc aattttttgc gcatttgcca cagccgatac aactttcggc attgcgacga
     1381 atcttaaacg gcgataatag cgaaaccacg cccatcagcg cgccatatgg gcaaagataa
     1441 cgacactagg catggcgaat aaacaggctg gcaatcagca aaacggtcac gctgattaat
     1501 gtcgcggtcc ccatatgacg aaagaaatcg agcattttta cgtccatcac cacgctgtag
     1561 ggcgacaaca taaaatagtg aatcgcctga gcgggcatca ataacgcgat atagagaaaa
     1621 aaactcaaca gcaaatactt cacgccgcgc agaggaatat ccagccagcg gggaaggaca
     1681 cattgccgac caaacagttt gttaccgaga tcgccgatta attcagaaag cgtaccaacc
     1741 gggcataacc atgagcaaaa ggcctttttg agtaatagac tgatgacgat aaaagcgacc
     1801 aataacagca tcgcggcggt gtggacggac ggtaactgac ctgttgtaag gctatatttc
     1861 agattcatca gaccggcaat cggtagccag ccttcgatac ctcccggtct ggcgacaaat
     1921 gtcgtgctac ttgccgtttc gtaatagcgc acccaatacc agaacgtgat ggcaatataa
     1981 atattcattg ccaacagtaa taattgcgtc gctttacgcc aggtcgtggc attgcgccag
     2041 tcattccacg gtaatttgcc gcccgtcgtg cctggccgcc gctgccagcg ggttcttttt
     2101 ttctctgcca tgattttgcc agtccgttaa gttgcatacc aaatgccact attctagttg
     2161 ttcttaactg gctgatattg attcaaatcg cgttcaggtc tttcttatgc aaccatgctt
     2221 ccagagcggc aacactgcgt gtaatttctt cgtgtggaag gggggcagat aatggctgct
     2281 gctccagttg tgcgcatagc tggctggcga tacgcattcc caaactcaag caactgcttt
     2341 ttagctgatg cgcggcacgc tttattttct cgctatcctg actggcgcgg gcaatgtcga
     2401 tttcatcgag aagcggcagg gcatgttgtg taaataatac cagccattcg tggatcttct
     2461 ccgtccccat taactgagca tcttcattga gttgcgatac atccagcgat tgatcgttat
     2521 tgacttgcag ttggagatag tgcgccagta actgaccgag cacttcacgc ggcaccggtt
     2581 tagggataat cccgcggaat aatgaactgg tacgctggcg cagcgtttcg tcaatgacat
     2641 gggcgctaaa gccaatcaaa accagcgacg gatattgctg tgccagttgt cgggcaagcg
     2701 taatgccgtc gacatccggc agatcaaaat ccaccagtgc ggcagcaaac ggttcgctat
     2761 tttgcagtgt ctctaaagcc tgcgcggcat tgccaacagc aacaacctgc gcaccactgg
     2821 tgttcagcat ctcaacggta attcgctggg ttagcgggtt atcttcaatt aacagcaaac
     2881 gtaaaccgtc aagacgcacc gcctgattga ctgtttttgg cacgggtgcc gtggcaacac
     2941 gtaacggcaa gcgtaaacaa aaacagcttc caacctccgg cgtgctggtg gcgctcagtt
     3001 cgccgcccat cgcctgggcc agacggctac tgatagtcag ccccagcccg gtgccgccgc
     3061 gtttgccgct tacctgcaca aatggctgga agatttctgc cagtttcgcg ggatcaatac
     3121 cgcagccgct gtcttccact tcgaccagcc attgctcgcc atcagtgcga ctacgcagga
     3181 taatgtaccc ttcgtcagta aaacgcaggg cgttgctcaa caggttggtt ataacctgac
     3241 gaatacgtcg tggatcgccc attaacgcgc acggcatatc atcggcaatt gccgttgcca
     3301 ggcgaatcgg gcgacctttc acccgtccgc tcattaattg cagggtactt tccagcagcg
     3361 ggcgcggttc aaagggctca tcgctgaccg aaacattctt gccacctgct tcgatagcgg
     3421 aataatcgag aatatcgttg aggatggtca gcaacgattc accagagtca gtaattgccc
     3481 gcaaatcatc acgctgggcg ttaagtgcgg ggttatctgc cagtagctgt gcggtgccga
     3541 gaataccgta cagcggtgtg cggatctcat ggctcatcgc cgccagaaac gccgattttg
     3601 cctggctggc tttttctgct tccgcccgtg cctgtcggtg ttctatcact agttcctgca
     3661 attcagctgt acgcgctttg acctgcgccg ccagctgttc acggtggcga ttcagtgcat
     3721 gaacactgct gcgaaacgca cccatcagct gcccgatggt gtccagctcc cgtacgccag
     3781 cggtttccgg gaaaggggag tcaatatcac cgtccagcag ccgttgcagc gcctgcgttt
     3841 gttcggcaag tggacgcgtg actgagcgat aaaccacgcg ccagaggatc agaatcagtg
     3901 cgcaaagtga aaccatcccc agcaataaca ggctgtattg cccgcgtgca ctggcttttt
     3961 cctgatgcgc cagtccgtgc tgattacgca gctcaatggt gtcgaccagc tgactgactt
     4021 cgctactaaa ctgcgcgaac tgggcgatat tattttgggc gagagtttgc aggtgattgc
     4081 tgatttcact gtcctgctgg aacagcgcca acagatcgct atattggcta acggtagtta
     4141 acgttgttgc gacctgcgca cgaactcccg gatcttcaat gcgtatttgc cgacgttgca
     4201 gaattttcac cgcattattg agctgctttt ccagcgttgg tgcatttttc tggatctgct
     4261 ccagccccag attcatcacc atttgctgca cccgcagagc gctaaggcgc agttcattca
     4321 tctggttaac atactcaaga tcgatatcaa tcagccgatc gagtgcactt tcagcagcct
     4381 gacgctgatc ttgttcgatc aaatcgtaaa tcccggcctg ggtcgctcca gcggaagttg
     4441 tcgcattatt cgcctgacct tgcgccaggc gtgcgatctc atcggcggca gcgactatct
     4501 gctgactgag ttgctgttgt tgctggcgta gttgcagacg ccgccccacc agttcccctt
     4561 gctgacgtaa cgaacgggag atctcctgct cctgttgttc aatagcggtg gtatcaaaac
     4621 cttgttcccg taacgcttgc agcaacgcat taatcttcag gctttgtgcg gtgagcattc
     4681 gcccctgcgc ctgccacatc ttttcgttat cggcactggt caggttctgc gcggcgaaaa
     4741 gttcccaggc gctggcttcg ctcaactggc gcgccatatt catggtagga atcaatgcct
     4801 gagtattgtc tttttccacc tggctgataa agcgcaggtt gtaccatccc accagggtac
     4861 tggtcagggt taacagcgcc atcagggcaa agcccatcca gagtcttcgg gtcagggtta
     4921 aattcacggt cggtgcactt taggtgaaaa atgttgagtc gcaaagcgga atgcatctag
     4981 cataaagcct tattattgat gaggctatca tgcgcgtact gctattttta cttctttccc
     5041 ttttcatgtt gccggcattt tcggctgata acctgttgcg ctggcatgat gcgcagcatt
     5101 tcacggtgca agcctctatg ccgcttaaag ccaaacgcgc atggaaactg tgcgcgcttt
     5161 atcccagcct gaaagattca tactggttat cgttgaacta tggtatgcag gaggcagctc
     5221 gccgttatgg tgtggattta aaagtgctgg aggcaggcgg ctacagccag ttggctaccc
     5281 agcaagcaca aatcgaccag tgtaaacagt ggggcgcaga ggccatttta ctcggtagta
     5341 gcacgacctc atttcccgat ctgcaaaagc aggtagcaag tctgccggtg atcgaactgg
     5401 taaatgctat tgatgctccc caggtgaaaa gccgcgttgg tgtaccctgg tttcagatgg
     5461 gctatcaacc tgggcgatat ttggtgcaat gggcgcacgg taaaccactg aacgtgctgt
     5521 tgatgcccgg acccgataat gccgggggca gtaaagagat ggtcgagggt tttcgcgcag
     5581 ccattgccgg aagcccggtg cgtattgttg atattgcgct tggtgataac gatattgaaa
     5641 tccagcgtaa cctgttgcag gagatgctgg aacgccaccc agaaatcgac gtcgttgccg
     5701 gaacggccat tgcggcagag gcggcaatgg gtgaagggcg taacctgaaa acgccgctta
     5761 ccgtggtgtc gttttatctt tcacatcagg tgtatcgcgg gctgaagcgg ggaagagtga
     5821 ttatggctgc cagcgatcaa atggtctggc agggggaact ggcggttgag caggccatca
     5881 ggcaattaca ggggcaatcg gtgtctgata atgtcagccc accgatttta gttctgacgc
     5941 cgaaaaatgc cgaccgtgaa catattcgcc gctcgctgtc accaggggga tttcgtccgg
     6001 tctattttta tcagcacaca tcagcggcta agaaataacc ttcaccatgt tgcgtcacca
     6061 gtaaatccgc gctgagttta tgacgtaaac gacgaattaa cacatcgacg gtgcgcaggt
     6121 cagggttttc cacccgacgc gcagaaagca tacgtagcag acgttcacgg ctgagaattt
     6181 cgcccggatt cgtcacaaat gccaccaaca tttcatactc tgcgcgggtc agtttaatcg
     6241 gctcgccatc ccgctccagc gtatggcgcg acacattcag gcaataaccg gcaaagcgat
     6301 agcagttgtc ctgagtgtgc ggttgagctt gtcgcgcgag gtcgattcgc cagagcagat
     6361 ttttcacccg tactaccagt tcgcgcagtt ccagcggttt ggtgacgtaa tcgtctgcgc
     6421 ccatttccag cccaacaata cggtcaatcc gatcgctgcg tccggtaacc agaataatcc
     6481 ccaccgttga gcgttctcgc agggcgcggg ttaacatcag gccattttca tcgggtaagt
     6541 tgatatccag cagaattaaa tctaccggct gattctgcat aatttcccgc agcccggcac
     6601 cgctcgctgt aacggaaacg gtatacccct cctgagtgaa gtaagattgt aatcgcgcct
     6661 gggtaaccgg ctcatcttca acaataacaa tgtgatgtgg catcagaggg ttttactcat
     6721 tctgttcata ttctgccgta agccgttcat cctgaccagt gccgctgttc atatttgctc
     6781 attaagatcg cttcactaaa ccataatttt acaggggcta ttatgcggaa actctggaac
     6841 gcgctacgcc gacccagtgc tcgttggtcg gtactggcgc tggtcgctat tgggattgtg
     6901 attggcattg cgctgattgt attgccacac gttgggatca aagtcaccag cacaaccgaa
     6961 ttttgtgtca gttgccacag tatgcaaccg gtgtatgaag aatataaaca gtcggtgcat
     7021 tttcagaacg cctctggcgt gcgagctgaa tgccatgact gtcatatccc gccggatatt
     7081 ccaggcatgg tgaagcgcaa actggaagcg agcaacgaca tttaccagac ctttattgcc
     7141 cactccattg atacacctga aaaattcgaa gccaaacgcg cggaacttgc cgagcgtgaa
     7201 tgggcgcgaa tgaaagaaaa caactcggca acctgtcgct cctgccataa ctacgatgcg
     7261 atggatcatg ccaggcagca tcctgaagcg gcacgtcaga tgaaggtggc agcgaaagat
     7321 aatcaatcct gcatcgactg tcataaaggt attgcccacc agttaccgga tatgagtagc
     7381 ggcttccgta agcagttcga tgagctgcgc gccagtgcta atgacagtgg tgacacgctg
     7441 tactctattg atattaagcc aatttatgcg gcgaaaggcg ataaagaagc ctctggttct
     7501 ctgctgcctg cttcggaagt gaaagtcctt aaacgtgacg gcgactggct gcaaattgaa
     7561 attaccggct ggacggaaag cgccggacgt cagcgtgtac tcacccaatt cccaggtaaa
     7621 cgcatctttg ttgcctcgat tcgtggtgat gtgcagcagc aggtgaaaac gctggagaaa
     7681 accaccgttg ccgacaccaa taccgagtgg agcaagttgc aggccaccgc gtggatgaag
     7741 aaaggcgaca tggtgaacga tatcaaaccg atctgggcct atgcggattc gttgtacaac
     7801 ggcacctgta accagtgcca cggcgcaccg gaaatcgccc actttgacgc taacggttgg
     7861 atcggcacgc tcaacggcat gattggcttt accagcctcg ataaacgtga agaacgcacc
     7921 ttgttgaaat atctgcaaat gaatgcgtct gacaccgcag gtaaggctca cggcgataag
     7981 aaggacgaaa aataatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
     8041 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgtta acgccgcgcc
     8101 gtgcgactgc ggcgcaagcg gcgactgagg ctgtcatctc gaaagagggc attcttaccg
     8161 ggtcgcactg gggggctatc cgcgcgacgg tgaaggatgg tcgctttgtg gcggcaaaac
     8221 cgttcgaact ggataaatat ccgtcgaaaa tgattgccgg attgccggat cacgtacaca
     8281 acgcggcgcg tattcgttat ccgatggtac gcgtggactg gctgcgtaag cgccatctca
     8341 gcgatacctc ccagcgcggt gataaccgtt ttgtgcgcgt gagctgggat gaagccctcg
     8401 acatgttcta tgaagaactg gaacgcgtac agaaaactca cgggccgagt gccttgctga
     8461 ccgccagtgg ttggcaatcg acggggatgt tccataacgc ttcggggatg ctggcgaaag
     8521 ctattgcctt gcatggtaat agcgttggta cgggcggaga ttactctacc ggtgctgcgc
     8581 aggtgatcct gccgcgcgtg gttggctcaa tggaagtgta tgaacagcaa acctcctggc
     8641 cgctggtatt gcagaacagc aaaaccattg tgctgtgggg ctctgatttg ctgaaaaacc
     8701 agcaagcgaa ctggtggtgc ccggatcacg atgtttatga atattacgcg cagctaaaag
     8761 cgaaagtcgc cgccggtgaa attgaggtca tcagcatcga tccggttgtc acatccaccc
     8821 atgagtatct ggggcgcgag catgtgaagc acattgcggt taacccgcaa actgatgtgc
     8881 cgctgcaact ggcgctggcg tatacgctgt acagtgaaaa cctgtacgac aaaaacttcc
     8941 tcgctaacta ctgtgtgggt tttgagcagt tcctgccgta cctgctgggt gagaaagacg
     9001 gtcagccgaa agatgccgca taggctgaaa aactgaccgg cattgatgcc gaaaccattc
     9061 gtgggctggc gcggcagatg gcggcgaaca gaacgcaaat tattgctggc tggtgcgtgc
     9121 agcgtatgca gcacggtgaa cagtgggcgt ggatgattgt ggttctggcg gcgatgctgg
     9181 ggcaaattgg cctgccaggt ggtggctttg gttttggctg gcactataac ggcgcaggca
     9241 cgccggggcg taaaggcgtt attctgagtg gtttctccgg ctctacgtcg attccgcctg
     9301 ttcacgacaa cagtgattac aaaggctaca gcagcaccat tccgattgcc cgttttatcg
     9361 atgcgatcct cgaaccgggg aaagtgatca actggaacgg taaatcggta aaactgccgc
     9421 cgctgaaaat gtgtattttt gctggaacta acccattcca tcgccatcag cagatcaacc
     9481 gcattattga aggctggcgc aagctggaaa cggttatcgc catagataac cagtggacct
     9541 caacctgccg ctttgccgat atcgtgctgc ctgcgaccac gcagtttgag cgtaacgatc
     9601 tcgaccagta cggcaaccac tccaaccgtg gcattatcgc catgaaacag gtggtgccgc
     9661 cgcagttcga ggcgcgcaac gactttgata ttttccgcga gctgtgccgt cgctttaatc
     9721 gcgaagaagc ctttaccgaa gggctggacg aaatgggctg gctgaaacgc atctggcagg
     9781 aaggtgtaca acaaggcaaa ggacgcggcg ttcatctgcc agcgtttgat gacttctgga
     9841 ataacaaaga gtacgtcgag tttgaccatc cgcagatgtt tgttcgccac caggcattcc
     9901 gcgaagatcc ggatctcgaa ccgctgggca cgccgagtgg cctgattgag atctactcga
     9961 aaaccatcgc cgatatgaac tacgacgatt gtcaggggca tccgatgtgg tttgagaaaa
    10021 tcgaacgctc ccacggtggg cccggctcgc agaagtatcc gttgcatctg caatctgtgc
    10081 atccggattt ccgacttcac tcgcagttat gtgagtcgga aactctgcgt cagcaatata
    10141 cggtagcggg taaagagcca gtattcatta acccgcagga tgccagcgcg cgcggtattc
    10201 gtaacggtga tgtggtacgc gtctttaacg ctcgcggtca ggtgttggca ggggcagtgg
    10261 tttctgaccg ctatgcaccc ggcgtggcac gaattcacga aggggcatgg tacgatccag
    10321 ataaaggcgg cgagcctggt gcgctgtgca aatacggtaa ccccaacgtg ttgaccatcg
    10381 acatcggtac atcgcagctg gcgcagttgt tctcccgtga actggacgat gaacaactga
    10441 cgcaaatcgc cagtgcgcag atggctgaat ggttttcgtt gctgaaaagc gaaccgccgc
    10501 tcactgcggc ggtgaatgcg ctggaaaacc gtattgccgc tctgacagta cgtgacgatg
    10561 cccgtctgga actggccgct gacttttgcg gcctgtttct gatgaccgac aaacaagcgg
    10621 cgctgccgta tgcatcggcc tacaaacagg acgagcaaga gattaaacgc ttgttagttg
    10681 aggcagggat ggaaaccagt ggcaatttca acgaatcggc agatcatctg gcgatctatc
    10741 tcgagttgtt gagtcatctg catttttcgc tgggagaggg gaccgttcct gcgcgaagaa
    10801 tcgacagttt gcggcaaaaa acactgacgg cgctgcggca atggttacca gagtttgctg
    10861 cgcgttgtcg acagtatgac agctttggtt tttacgcggc actaagccag ttattgctgg
    10921 tgttagtgga gtgcgaccac caaaacagat aacgtcgttt atgcgcctga aaagacgcgt
    10981 ttagcgtcgc atcaggcatt atggcgcagt tgccggatgc gtcatcacat ccggcaaaca
    11041 cacatcacgg atgagctaca aaccgggaaa gccgctggcg tagcaggcgg ttttcctgct
    11101 tcaggtgcgc aatatcatcc attaacgtca gcgccaccgc gatccccggc cagtccagag
    11161 ccagttcatg acgcaggcgt accgcgcgtt gcaccacaat ggcggcatgg tcgtcaaata
    11221 cccaggttgt ttcctggatc tcacgcggtt caaccacccc caaaccgaca atttcattca
    11281 actcctcttc agagatgccg gtatgcaggc aaaattcggt aatagtaaaa gtcaccgtaa
    11341 cattagccat catgctttcc cccaatcttt acgtggatca aaagacgact gggcgtctgc
    11401 cagttgctgc cacagcgcgg cagtgttttc atccggtttc ggcggcatca cgattttcag
    11461 taccgcatac agatcgccgg tctgtttttt gctcaccaga cctttgcctt taacgcgcaa
    11521 tcgttgcccg gcctggctgc ctggcgggat agtcagcaaa atgctttctt tcagtgttgg
    11581 aacggtgact ttagcaccca gcgccgcttc ccacgggcta accggcacca caatttccag
    11641 atcctggccg acaatatcga acagcggatg tggcgcaata tgaatcacca gccacaaatc
    11701 gccatttgga ccaccgtttt cgcccggcgt cccctggcct ttcagacgga tgcgttgacc
    11761 attgccgacg cccgccggga tcttcacatt cagcgttttc gggatttcct gttcgatcat
    11821 gccaaaggcg ttataaaccg gcaggttata gctgatggta cgcttatgct cagtcagcgt
    11881 ttcttcgagg aataccgcca cttcgatttc aatatcgtgg ccgcgtgcgg cggggcgttg
    11941 acggctctgg cgggcatgct gaccgaaaat tgacgagaag atatcgtcaa aatcttcggc
    12001 attaaaactc tgaccgtcgc catgatggaa ctgatggtta aattgcggat cgttgcgatg
    12061 ttgccacatc tgatcatact cagcgcgacg ttgttcatca cttaacactt cccaggcttc
    12121 agcgacctct ttgaagcggg cttcggcatc cggttctttg ctgacatcag gatggtattt
    12181 acgggccagt cgacgatagg cggtcttgat tgtcttgaga tcgtccgtcg gtttcacgcc
    12241 catgatggcg taataatcct ttaattccat agcgttatct cgcgtaaatc aacacaaatt
    12301 gaaggaaccc ctgtaaggta actcctataa gtgtagggta atcctcaaaa tttcatatgc
    12361 caacacagaa tatgttattg aaatcatcgc ggagaggagg tcgccatcaa gatgggttgc
    12421 tgaacatatt ttaaacaggt gaaaaagggt gagcgatttt tgatagttga accaggcact
    12481 ttaagtt
//
LOCUS       DAEEKQ010000114        12360 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.114, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000114 DAEEKQ010000000
VERSION     DAEEKQ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12360
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.114"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(121..330)
                     /locus_tag="KLK57_002968"
                     /pseudo
     CDS             complement(121..330)
                     /locus_tag="KLK57_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="KLK57_002969"
     CDS             complement(532..1176)
                     /gene="cheZ"
                     /locus_tag="KLK57_002969"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HBE0893278.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGLVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKVGVVASQDQVDDLLDSLGF"
     gene            complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="KLK57_002970"
     CDS             complement(1187..1576)
                     /gene="cheY"
                     /locus_tag="KLK57_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HBE0893279.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="KLK57_002971"
     CDS             complement(1591..2640)
                     /gene="cheB"
                     /locus_tag="KLK57_002971"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HBE0893280.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="KLK57_002972"
     CDS             complement(2643..3503)
                     /gene="cheR"
                     /locus_tag="KLK57_002972"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HBE0893281.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHAHRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGYSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3522..5123)
                     /gene="tap"
                     /locus_tag="KLK57_002973"
     CDS             complement(3522..5123)
                     /gene="tap"
                     /locus_tag="KLK57_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HBE0893282.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISTLVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5169..6830)
                     /gene="tar"
                     /locus_tag="KLK57_002974"
     CDS             complement(5169..6830)
                     /gene="tar"
                     /locus_tag="KLK57_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HBE0893283.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHIREIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSAQAAKEIKALIEDSVARVDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRIAEQDPNWETF"
     gene            complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="KLK57_002975"
     CDS             complement(6975..7478)
                     /gene="cheW"
                     /locus_tag="KLK57_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HBE0893284.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="KLK57_002976"
     CDS             complement(7499..9463)
                     /gene="cheA"
                     /locus_tag="KLK57_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HBE0893285.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSMAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVGDEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9468..10394)
                     /gene="motB"
                     /locus_tag="KLK57_002977"
     CDS             complement(9468..10394)
                     /gene="motB"
                     /locus_tag="KLK57_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HBE0893286.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEDLKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRVIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10391..11278)
                     /gene="motA"
                     /locus_tag="KLK57_002978"
     CDS             complement(10391..11278)
                     /gene="motA"
                     /locus_tag="KLK57_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HBE0893287.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="KLK57_002979"
     CDS             complement(11404..11982)
                     /gene="flhC"
                     /locus_tag="KLK57_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HBE0893288.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="KLK57_002980"
     CDS             complement(11985..12335)
                     /gene="flhD"
                     /locus_tag="KLK57_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HBE0893289.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         2831 a         3451 c         3021 g         3057 t
ORIGIN      
        1 gagtcggcga attacatagg ggctcgcagc gttacggctg caataaagcc ggatataaag
       61 ctgcaaccta cccgtcatgt caaaacaatg gatgccttgc aaacgggatg cgttcatata
      121 aatactgtga tcaaaatgca gcccagcgga gagcatgccc gacaatcgac gggccagcga
      181 cacaccgcca aaccagataa cacacacgcc cactaacaaa atcagcaacg aggtcagttc
      241 acgggaacgc gggatttgcc cctcttcccg cgctttttct agtcggtgag gtgtgggggc
      301 ttctgttttg tcgtcgctct cgtcagacac gtcgccaatc ctggaaagag tattaagctt
      361 aaatgatgcc agagcgcaaa gcgttcaatg gtttgagtaa ggggcaaaac aggcgggatt
      421 tagggctttt gctgccacac atcaagcata gtgtgcgttt gtcggatgcg gcgtcatcgc
      481 cttatcagac cgcctgatat gacgtggtca cgccacatca ggcaatacaa atcaaaatcc
      541 aagactatcc aacaaatcgt ccacctgatc ctgactggct accacgccga ctttgctggt
      601 atcgacctga ggtccattaa gcaaactctg gttttcacgt tttggacgcg actcctgttc
      661 cgggatgttt tccaacagca ccatcagcaa ctggcgttcg atctcctgaa tgacatccat
      721 catccgctta atgaccagcc cggtgagatc ctgaaaatcc tgcgccatca tgatttccag
      781 cagttgcgcg ttagtaaagc tggtatgcgc gggtacatct gccagaaatt gtcgtgtatc
      841 tgttaccagt tcacgggcat cggcaaggtc aatcggatcg gcaaaccagt catcccaacg
      901 ttgggttaac gcttttgctg atttctccat ttgatcctga tgcggttgtg acgcctcaac
      961 actgttcagc gcccgctccg cagcctgggc ggtcatctgc acaacatagt acaaacgatc
     1021 gcgcgcatcg gggatggctt ccgccgcttc ggcaatggcc tgatccagcc ccagttcccg
     1081 caaactgtcg cgcagcatac gcgtcaggct gccgatgcgc gcaatgatat cgccagctga
     1141 atgctcgtca gcaggtttga ttgatggttg catcatagtc gcatcctcac atgcccagtt
     1201 tctcaaagat tttgttgagt ttttcctcca gcgtcgcggc ggtaaatggc ttcaccacat
     1261 agccactggc ccccgcttgc gccgcagcaa tgatgttctc tttcttcgct tctgcagtca
     1321 ccattaacac tggcaatgcc gacatcgcgc catccgcacg aattgttttc agcaattcca
     1381 ggccatccat attgggcatg ttccagtcgg agataacaaa tccataaccg cctgcctgca
     1441 acttattgag agcgtcgacg ccatcttccg cttcctcaac attattgaat cccagctctt
     1501 tcagcaggtt acgcactatg cgtcgcatgg tggaaaagtc atccacaacc aaaaatttaa
     1561 gttctttatc cgccatttca cactcctgat ttaaatacgt atcgcctgtc cggcactaat
     1621 ttttgccaac atttgctggc ttacctggct aagatcgacc acttcgcaga caccacccat
     1681 attgatggcc tcgcgcggca tgccgaacac cacgcaactt gcttcgtttt gcgcaagggt
     1741 ccatgccccc gcctgacgca tcgccaacat tcccgccgcg ccgtcgttgc ccataccggt
     1801 caggatcacc ccaaccgcat tacgccccgc ctgtttggcg acagaatgga acaacacatc
     1861 taccgaaggc cgatgacggt taaccgccgg gccgtcgtga attttgattt gataatttgc
     1921 gccactacgc gccagttcca tatgccgatc gcccggcgca atataggcat gccccggcaa
     1981 gacacgttct ccgtcttcgg cttctttaac cccgatctgg caaagcttat taagtctgtc
     2041 ggcaaaagag cgggtgaaac cgggcggcat atgttgggta attaacaatg ccgggctgga
     2101 aagcggcaac ggttgcagta cgtgacgaat tgcctcagtt ccacccgttg aagcaccaat
     2161 cgcaatcagt ttttcagaac tcaacaacgg acccgccttc agcgttgtcg gtgctgacaa
     2221 tggcttatgt gctgcaaggc tcgcctttgc tgccgtacgc accttttcag caatcatttc
     2281 gctatacgcc agcattcctt cgcgaatacc cagttgcggt ttggtgacaa aatctattgc
     2341 ccccagctcc agcgcgcgca gcgtgacttc tgatcctttg ccggtcaagg aagaaaccat
     2401 cacaacgggc attggacgca aacgcattaa tttttcgagg aaatccagtc cgtccatccg
     2461 cggcatttca acatccagcg tcagcacatc gggattgaat ttcttaatca agtcacgcgc
     2521 gaccagcgga tcaggcgcgg tcgccaccat ttccatgtcg ctatggctgt tgatgatttc
     2581 tgtcatgatc tggcgcatca gtgccgaatc atcgacagat aacaccctga ttttgctcat
     2641 cgttaatcct tacttagcgc atacaccgtc tgaccacgca gcgtgaagcg gcgctcaagg
     2701 tggctaaagt tttcagagta acccgcaaac agcaatccgt cgggtttaag gagcggaaca
     2761 aagcggcgca aaatctcctg ctgggtattt tgatcgaagt agatcatgac gttacgacag
     2821 aagatcgcat caaacggccc cggcacggtg tactgtttcg ccagtagatt cagcggggca
     2881 aaatcaacat agttcgccag ctcctgacgc acgcgtacca gcccttcatg cggtcccgtc
     2941 cctcgcatga aataccgttg cagttgctgc ggcgtcaggt ttttcaactc ttcatggcga
     3001 taaataccgc ttctggcttt ttccagcact tcggtgtcga tatcactggc aaacactttc
     3061 cagcgtccgg gcgcggtgcc caatgtgtca gccagcgtca tcgcaatgct gtacggctct
     3121 tcgccggtcg aagccgccgc gctccatacg cgatactcgc cagaacggcg atgtgcgtga
     3181 tccgcgagca gagggaaatg gtgtgcctca cggaaaaatg ccgtcagatt cgtggtcagc
     3241 gaattgataa acgcctgcca ctcaccgctg tgctgattag attccagcaa gttcagataa
     3301 tgaccgaaat ccgtcagtcc cagcgaacgc aaacgacgaa ccagtcggtt gtaaaccatg
     3361 tcgcgtttat ggtcagccag aacgatcccg gctcgttgat agatcaattg acttatccgc
     3421 cgaaaatgcg cgtcggaaag cgccaggcgc tcggtcatct gtaacaataa agacgtttgc
     3481 ccacagggca gagatgaagt catagcgcct tctcaatcac ttcaggatac cactggcgca
     3541 atttgtaact gcgccgactc atgtcgtgct acttcatgtt cttcaagggt aaataccgcc
     3601 acacgcgacg aaagatggtc ggcctgattc gccagttgtt ccgttgccac cgccgcttct
     3661 tctaccagcg aggcgttctg ctgagtcacc tgatccatct ggctgacagc ctgtgcaacc
     3721 tgctcaatcc cccgctgttg ttcttccgac gcagaggcaa tttctcccat aatgtcgttc
     3781 acgcgggtca ccgaactgac aatatcgatc atggtcgcgg cggcgttatt caccagtttc
     3841 gaaccctgct ggacacgatt gactgactct tcgatcagcc ctttgatctc ttttgctgcc
     3901 tgcgcgctac ggctggcaag attgcgcact tcacctgcca ctaccgcaaa accacgcccc
     3961 tgctctccgg cgcgagccgc ttccactgcc gcattcaggg ccagaatatt ggtctggaaa
     4021 gcaattccgt cgataacgct gataatgtcg ccaatttttt gcgagctggt ggcgatctcc
     4081 tgcatggtgt gagtcatggt actgacctga acaccgcccg cctgcgccgt tgtcgcggca
     4141 ttttttgcca gttccgacgc ctgtcgtgcg ttatcggcgt tttgccctac cgtggcggtt
     4201 aattgctcca tactggcggc cgtttgtgcc agcgatgccg cctgctgttc ggtacgactt
     4261 gagagatcgt tattgcctgc gacaatctcc gcgataccaa tgtgcatctc ctggcttccc
     4321 ttacgcacat cacttaccgt cccacgcaaa gcctgctgca tggtcttcag actggcaaaa
     4381 atggcggtga tctcattacg accatatacc gcaatcggac gcgccagatt acccgcagca
     4441 atgctgtcaa aatggctacc gataatggcc agtggttgaa caatcatctt gcgcgtccac
     4501 cacagcgcac tgctgatgta gatcgccgca acaataatca tgctgataaa caccagtgtc
     4561 gaaatctgat agttacgctg gctttgcgca ctggcggctt ccagcacatg gttgatctcc
     4621 agctgccagg cctcaaagtt aacgtcaaac gcattctgtg atccctgcac cggcgcagtg
     4681 aggaaatccg aaagttgatt actttcaagc caggtcgcct gatgttcgag atcgttgtgc
     4741 cagcgggcaa aacttttctc cgtctctttt tgcaatgccc tgacgtgctc gttgcccgca
     4801 gtcatcgcca taaaactttt aaacagcgtg gtggattgcg tcagactggc gcgcgccgtc
     4861 gtcatcaacg ttttaatgtc atccgccgga tagctaagtg ccgtcagagt gcccgctttg
     4921 ttcagcgcgg tactggcctg taacattacc gcccgagttt gcgctaatgc cgcacgttgc
     4981 tgattgctct gctcgacctg attcagtcgt tgcaaatcgt cgcgaaatgc ccaaaaagac
     5041 atgccgttac tgccaatctg caagatcccg cagagaatca aaattaaaaa cagcgtggtc
     5101 gaaattcgaa tacgattaaa catcaacgct ccccatcagg cggcaatgac cgcgttagta
     5161 aatactcgtc aaaatgtttc ccagtttgga tcttgttcag caattcgcag tcgtggctgt
     5221 gccggtggtt gctcactggc aggacgggat ggtgtttgcg gtttattggt gagtgggctg
     5281 gctgccagac ggaacgcgga aaccgcttgc gttaaacgac tcgcctgttc ttccagcgca
     5341 gcggcggcgg cagctgattc ctgcaccagc gatgcgttct gttgcgtgac gcgatccatt
     5401 tccgaaaccg ccaatgcgac ttgatcgatg ccacggctct gttcatccga tgccgatgca
     5461 atctcgccca taatgtcagt cacgcgagtg acagcattga cgatattgtt cattgtttcc
     5521 ccggcgcttt cgaccagcac cgaaccggta tcaacgcgtg cgacggagtc ttcaatgagg
     5581 gctttgatct cttttgccgc ctgggcgctg cgactggcaa gattacgcac ttcacccgcc
     5641 accacggcaa aaccacggcc ctgttcaccc gcacgcgcgg cttcaaccgc ggcattcagc
     5701 gcgaggatat tagtctggaa ggcaataccg tcgataacgc tgataatgtc ggcaattttc
     5761 ttcgaactat cggcgatctc atgcatcgtt ttcactacgc catccaccac tttgccgccg
     5821 tgctgggcgg tgtcggaggc actttgcgcc agttgcgagg cctggcgggc gttatcggcg
     5881 ttttgcttca ctgtcgcggt gagctgctcc atgctggcgg cagtttcttc cagcgcggat
     5941 gcctgctgtt cagtacggga ggaaagatcg gtgttgcccg ccgcaatttc acgggtaccg
     6001 gcatagatgg catctgaacc ttcgcggaca tgagtgacgg tgtcagtcaa agagcgttgc
     6061 atatgtgaaa cgctctgcgc caggtcgccc atttcactgc gcccgtcaat ggtcagggta
     6121 ttcgctaggt taccaccggc gatttcgcga atgtgagcaa taatttttgc cagcggagta
     6181 agcaacatac ggcgaatgcc gtaccacgcc accagcagaa tcaataccac caccagcgcg
     6241 ataaccgcca gttgccactg ggcaaatcgg taatcatctg cgttgtcagt gacgatatcg
     6301 cgatacagtt tttcactgct gagggcgtac tgagcaaacg cttcgcccat tgcattttgc
     6361 attccctggg ttggctgagc gaaataagct ccagtattgc cataatctag ataatcaatc
     6421 agttcagtta acgctgtgta atagttttta tatttttcat caatattacg actggtagcg
     6481 accatttcag gtaacggtgc catgcttttg aattttttat aatgcgtcgc tgcctgcgcc
     6541 aatgttttcc tggcgctatc gagcaattca actttggcgt tactttgttg attggaggaa
     6601 tccatcatca tccgcaccgc tgaacgactc aggttaatgc gcgtttgcag cattaaatcc
     6661 caggttgacg tcagctcgcc ctgctgttcc cgcaattgat tggaaaccac aaagctcttc
     6721 tggctatggt gaagggaaga aaaaaacaga ctgccggaaa taagctgtaa cagtgcgaat
     6781 acccccagca ccattaccaa cagcgtgact acgcggatac ggttaatcat aaggcacctt
     6841 cctgaaaaca agttgatctc gttatcggca agaagggggg aaactttatt gctgatgcca
     6901 cccgccgcga aattgaaata aaaaacccga tgcgcagatc atcgggttca tttcaattga
     6961 ggaaatcggg agaattacgc cacttctgac gccgcgctat ctaacagcgc catctcttcg
     7021 ctgttcagca gtttttcgat gttcaccaga atcaacatac ggtcgcccag tgcgcccagt
     7081 ccagtgagat attctgttga aagcgtcacg gcaaactctg gtgccggacg aatttgctcc
     7141 gccgtcaatg aaagcacgtc tgaaacgccg tcaaccacga tgccgaccac ccgctgtccg
     7201 agattcagga cgataactac cgtgttgtcg ttatagtcca catccacctg gctgaactta
     7261 attcgtaagt caacaatcgg cacaataacg ccgcgcagat tcgtgacgcc tttgataaac
     7321 gctggcgtgt tcgcaatccg tgttacctga tcgtagccac ggatctcctg cactttcagg
     7381 atatcaatac cgtactcttc atcaccaagg gtaaatacca gaaattcctg gcctgacggc
     7441 tcgctggcca gctttgttac attcgtcata ccggtcatat tgttaccttt ttactcattc
     7501 aggcggcggt gttcgccata cgttgttcgc ggtttatcgc ctgcaaggcg gagacatcaa
     7561 caatcagtgc cacgctgccg tcgccaagaa tggtcgcagc agaaatgccg gggactttgc
     7621 gatagttact ttcaaggttt ttaaccacaa cctggtgttg accaattaat tgatccacca
     7681 gcaaggcgta gcggcgaccg ccactttgta agatcacaac aattccctgg gtggcttcgg
     7741 ttttcgcgcc cgcgacgttg aacactttcc acagttcgac gatgggcaga tattcacccc
     7801 gcacttccag tacccgctcg ccgccggcca gtggatggag atcggcttca cggggttgca
     7861 gtgattccat aacagcattc agcggcagaa tgaaaacttc atccccaacg cgtacggaca
     7921 tgccgtcgag gatggccagc gtcagcggca gtaaaatgcg gatcgtagtg ccagtcccct
     7981 gcttcgactg gatttcgaca tgaccgccca tctcctggat attacgttta acgacgtcca
     8041 tgccgacgcc gcgcccggag acgtcggtga cctgctctgc catggagaag cctggtgcaa
     8101 atatcagcat cgcgacttcg tcgtcgctca tgttttcgct gacagtcaaa ccttgcgagg
     8161 ccgcttttgc cagaattcgc tcacggttta gccccgcccc atcgtcggtc acttcaatgc
     8221 aaatgttgcc gccctgatgt tcggcagaca gaattaaatt tccgacgctg tttttacctg
     8281 cggcgagccg tttttctggc agttcaatac cgtgatcgag gctattgcgt accaggtggg
     8341 tcagcgggtc gataatgcgt tctatcaggc ttttgtcgag ttcagtagaa ctgcccacca
     8401 gcgtcagttc tacctgcttg ccgagttttc ccgccagatc acgcaccagc cggggatagc
     8461 gactaaaaac atattccatc ggcatcatgc gaatcgacat cactgattcc tgcaaatcac
     8521 gggcgttacg ttgtaactgc cccatgctgg ttatcaaatc accatgatta accgggtcca
     8581 gttcgctgga acgctgggca agcatggact gggtgataac cagctcgccg acgaggttaa
     8641 ttaattgatc aaccttttct accgctacac ggatgctggt ggattcattg ctgcgcgtcg
     8701 ttttttcccg ctccacgcgg ccggttggcg cttgttcggc tgccagttta agcactggtg
     8761 gggtggatat ttttggcgag acttctactg tttcaaaggt aatctgatcg gcttcaatca
     8821 caaaacagag taccgctgtg atgtcatctt cggcgatgtc gcccggtaat attgccgaga
     8881 gcgaatccgc ccctttcacc acgtcagtta acgttgtcag atgtcccagt tcttcttcca
     8941 gcaggtcgac ttccccggcc ttcaggcgcg aaaggataat tcgtcgcggc gactgactgc
     9001 gactctgctc atcttgcggt tcacttttgg caaccacact taatcgggtc actgcggatg
     9061 gcgtttcgcc tttcgcttct aatgccagtt gacgcaaggc ctggcagata taatcgaagc
     9121 tggcggcatc cggctcttgc gactgtttat aagcgtcgag ctgttcttgc atgatgtcct
     9181 tcgtttccaa aaacagattg ataatgtcgg tgttgagttg catctcacct cgtctggctt
     9241 catcgagcag gttttccatc agatgcgtgg tttcctgcaa aacgctgaag ccaaaagttc
     9301 ctgcccctcc tttgatcgag tgggcagccc gaaagatggc attcaattgt tcggcatctg
     9361 gcgcttccgg ctgcaaaacc agcagatgct gctccatgtc agccaacagt tcgtccgctt
     9421 catcaaaaaa tgtctgataa aaatcgctta tatccatgct cacgctgtca cctcggttcg
     9481 gctgatggca ttgtgggaac actgacctgt ggtgcaacct caggtttttc cagggcgctt
     9541 actggctcat tctggctttc ggcgttttca tgcaaaatgg cctgttcggc ttgtttgttc
     9601 agtaccagca ggctgatgcg acggttgacg gcatcatcag gtccgcgatc gcttaagcgc
     9661 atcgttgccg ccatgccgac gacacgtaac actttgccgc tatccaaccc tccgaccatc
     9721 agttcgcggc gggatgcatt ggcccgatcg gcagaaagct cccagttgct atatcctttc
     9781 tcaccgctgg cgtaggggaa atcatcggta tgacctgaaa ggctaatacg gttgggaata
     9841 ccgttcagta caggcgcaat gacacgcaga atgtcgcgca tatagggttc gacatcggca
     9901 ctgccggttc taaacatcgg gcgattctgg ctatcgatga tctgaatacg tagaccttcc
     9961 tggaccagat cgattttgag atggggacgt aacgcccgca gtttcggatc ggactctatc
    10021 aactggtcga gatcaccccg caatttccgc aatcgacttt gctccatgcg ttttttcaga
    10081 tcttcgatgt tcggctgctt attcacttcc ccctggcttt gggtgtaatc atcaccaccg
    10141 ccgggaattg ggctttcact attagaaatg cgatcgccgc ccgtaaccgc agtcgccagt
    10201 ggagtccgga agtactccgc aatctgaatc agctcttttg ggctggagat ggagatcagc
    10261 cacatcacca gaaaaaaggc catcatcgca gtcataaagt cggcataagc aatcttccac
    10321 gatccatgtg ctgccccgtg gcttttggct ttgcgtcgtt tgacgacaat aatcggatgc
    10381 gcttgattct tcatgcttcc tcggttgtcg tctgttgttg cggatttttc accgcacgca
    10441 catgctcttc cagttcaata aacgacggac gttcgctgga atagagcgtt ttgcgaccaa
    10501 actcaacggc gataggcggt gcgtaaccgt tcagattaga aagcagagtg actttgacgc
    10561 actgcatcat tttgctggtt tcggcgcttt tctgacgtaa aacactcgct aatggggaaa
    10621 taaatccgta agccaataaa atgccgagga aagtccccac catcgcatgt gcgataagcg
    10681 cacccagctc ggcggcagga cgatcggctg aacctaacgc gtgaacgccc cccattacag
    10741 ccgcgacaat accaaacgcc ggaagtgagt ccccgaccag cgccagactg ttcgccggga
    10801 cttctgcctc gctttcgtgc gtctcaatct cttcatccat cagggcttcg atttcgaagg
    10861 tgttcatatg accgctgata atcagacgca gataatcgac gataaaatca agcatgacgc
    10921 tatccgcgag gatgcgtggg tagctggcga agatctcgct ctcacgggga ttttcaatat
    10981 cacgttccag cgaaaacatc cccatctgcc gcgatttcgc catcaaccga taaagcagag
    11041 ccagcagatc catatacatt gctttggtgt atttggagcg acgaaacagc aacggcagcg
    11101 ccttcagcgt gcctttaatc gctttgccat tattgccgac gataaacgac ccaatccctg
    11161 caccggcaat aatcaccagt tcagcgggtt gatagagtgc tccaaggctt ccaccggtca
    11221 tcaaataacc gccgaaaact gtaccgagaa caaccaggta acctaataag ataagcacga
    11281 catcatcctt ccactgttga ccatgacagg atgttcagtc gtcaggcgtt aacgcgcgat
    11341 tggggcaaaa aaagcagcgg tacgtcgtta ccgctactgg aatgttgcgc ctcaccgtat
    11401 cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat cggcaggatt
    11461 ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc tgcaggcaaa
    11521 gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt tgcagctgga
    11581 aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg ttaatgccag
    11641 cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg ctttgatcac
    11701 cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat tacagaacat
    11761 cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg gcagcatgcc
    11821 tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc cgcgacttaa
    11881 ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtaatcaatt ccattgccag
    11941 ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc ccttttcttg
    12001 cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag catgatgccg
    12061 gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt aatcgtctgg
    12121 tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt aaccatttgc
    12181 ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag acgaaacata
    12241 gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga caagttgatg
    12301 tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa taaccaactt
//
LOCUS       DAEEKQ010000115        12035 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.115, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000115 DAEEKQ010000000
VERSION     DAEEKQ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12035
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.115"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            9..323
                     /locus_tag="KLK57_002981"
     CDS             9..323
                     /locus_tag="KLK57_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE0893290.1"
                     /translation="MLPPLFIMLAYLNLRAKLDHLPRDFRMGSRRTGIIVVSMLIAIF
                     AVGFVASTFPTGANILTIIFYNVGGIVIFLGFAWWKYSKYIKGLTAEERHIEATPASN
                     VD"
     gene            457..1534
                     /gene="ygjJ"
                     /locus_tag="KLK57_002982"
                     /pseudo
     CDS             457..1534
                     /gene="ygjJ"
                     /locus_tag="KLK57_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
     gene            1551..3902
                     /gene="ygjK"
                     /locus_tag="KLK57_002983"
     CDS             1551..3902
                     /gene="ygjK"
                     /locus_tag="KLK57_002983"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HBE0893291.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKITSNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWLSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYSKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGLYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWLPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            4128..6146
                     /gene="fadH"
                     /locus_tag="KLK57_002984"
     CDS             4128..6146
                     /gene="fadH"
                     /locus_tag="KLK57_002984"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HBE0893292.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRAVRERVGNDFIIIYRLSM
                     LDLVEDGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADMVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYHRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(6191..6607)
                     /gene="higA"
                     /locus_tag="KLK57_002985"
     CDS             complement(6191..6607)
                     /gene="higA"
                     /locus_tag="KLK57_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HBE0893293.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLADLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(6604..6918)
                     /gene="higB"
                     /locus_tag="KLK57_002986"
     CDS             complement(6604..6918)
                     /gene="higB"
                     /locus_tag="KLK57_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HBE0893294.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="KLK57_002987"
     CDS             complement(7203..8339)
                     /gene="rlmG"
                     /locus_tag="KLK57_002987"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HBE0893295.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            8424..8927
                     /locus_tag="KLK57_002988"
     CDS             8424..8927
                     /locus_tag="KLK57_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HBE0893296.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPITKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            9004..9687
                     /locus_tag="KLK57_002989"
     CDS             9004..9687
                     /locus_tag="KLK57_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HBE0893297.1"
                     /translation="MQPRLLLRICFSRRTLKITCLLLLVAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHVGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            9766..10752
                     /locus_tag="KLK57_002990"
     CDS             9766..10752
                     /locus_tag="KLK57_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HBE0893298.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTASLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLESEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            11035..12000
                     /gene="alx"
                     /locus_tag="KLK57_002991"
     CDS             11035..12000
                     /gene="alx"
                     /locus_tag="KLK57_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HBE0893299.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT         2861 a         3093 c         3225 g         2856 t
ORIGIN      
        1 cagcgtccat gcttccgccg ttattcatca tgctggctta cctgaattta cgcgccaaat
       61 tagatcacct gccacgcgat ttccgtatgg gctcccgccg caccggtatt atcgttgttt
      121 caatgctgat tgcgatattt gccgtagggt ttgtcgcttc gacattcccg actggcgcga
      181 atattctgac catcattttt tataacgtcg gcggtattgt tatcttcctt ggctttgcgt
      241 ggtggaaata cagtaaatat ataaagggat taacggctga agagcgccat attgaagcga
      301 cgccagccag caatgttgat taagcagaat aataaaaaat gagcaggcat tttccctctt
      361 cctggatgag gagagggaaa atagtttctg cctttatatt tttataatta caacgataaa
      421 aggctgtact ttttctttag ctcatggatt aacacaatga aattaatcac tgcaccatgc
      481 agagcattac ttgctctgcc gttttgctac gccttttctg cggcaggaga agaagcacgt
      541 ccggcagaac atgacgacac aaaaacaccc gcaattacct cgacatcttc tccttcattt
      601 cgtttttacg gcgaattagg gattaggggg tggtggatat atggatttag agggtgagaa
      661 taaacataaa tacagcgacg gtacctatat tgaaggtggc ctggagatga agtacggctc
      721 ctggttcggc ctgatttacg gcgaaggctg gaccgtgcag gccgaccacg acggcaatgc
      781 ctgggtgcca gaccatagct ggggtggttt cgagggcgga attaaccgtt tctatggcgg
      841 ttatcgtacc aatgatggca ccgaaatcat gctcagtctg cgtcaggatt cctcgctgga
      901 tgacctgcaa tggtggggcg atttcacccc cgatctgggc tacgtcattc ccaatacccg
      961 cgacattatg actgcgctga aggtacagaa cttaagcggc aactttcgtt atagcgtcac
     1021 cgcgactcct gccggacatc atgatgaaag caaagcctgg ctacattttg gcaaatacga
     1081 tcgctatgac gacaaataca cctatccggc aatgatgaac ggttacatcc agtatgacct
     1141 tgccgaaggc atcacctgga tgaacggtct ggaaatcacc gacggcacag gacagctcta
     1201 tctcacgggc ctgctaactc ctaactttgc cgctcgcgcc tggcaccata ccggacgcgc
     1261 cgacgggctg gacgtaccgg gaagtgaaag tgggatgatg gtgagcgcca tgtatgaagc
     1321 gttaaagggc gtttatctct ccaccgctta cacctacgcc aaacatcgcc ctgaccacgc
     1381 tgacgatgaa accacctctt tcatgcagtt tggtatctgg tacgaatacg gcggcggacg
     1441 tttcgccacg gcttttgata gccgcttcta catgaaaaat gcctctcacg atcccagcga
     1501 ccaaatcttc ctgatgcaat atttctactg gtaataagga ctgtaatttc atgaaaataa
     1561 aaactatttt aacgccagta acctgcgctc tgctgataag tttttccgcc catgccgcta
     1621 acgccgacaa ttataaaaac gtgattaacc gtactggcgc gccgcagtac atgaaggatt
     1681 acgattacga cgatcaccag cgttttaatc cgtttttcga tctcggagcc tggcatggtc
     1741 atctgttgcc agacggccct aacaccatgg gcggctttcc gggcgttgcg ctgctgacgg
     1801 aagagtacat caactttatg gccagcaatt tcgaccgcct gaccgtctgg caggacggca
     1861 agaaagtcga ctttacgctg gaggcataca gtattcccgg tgcgctggtg caaaaactga
     1921 cagcaaaaga tgtgcaagtc gaaatgactc tgcgcttcgc cacgccgcgc acgtcactac
     1981 tggaaaccaa aatcaccagc aataaaccgc tggatctggt gtgggacggc gaactgctgg
     2041 aaaaactgga agcgaaagaa gggaaaccgc tttccgataa aaccattgct ggcgaatacc
     2101 ctgactatca gcgcaaaatc agcgccaccc gtgatggcct gaaagtcacc tttggcaaag
     2161 tgcgcgccac ctgggatctg ctgacctccg gcgaatcgga atatcaggtg cataaatccc
     2221 tgccggtgca gactgaaatc aacggtaatc gctttaccag taaggcgcat atcaacggtt
     2281 cgaccacgct ctacaccacc tattcccatc tgctgaccgc tcaggaagtt agcaaagagc
     2341 aaatgcagat ccgcgatatt ctggcgcgtc cggcgtttta tctcaccgcc tcgcagcaac
     2401 gctgggaaga atacctgaag aaagggttaa ccaatccgga tgcgacgccg gaacagacgc
     2461 gcgtcgcggt gaaagcgatc gaaacgctca acggtaactg gctctcaccg ggcggtgcgg
     2521 tgaaatttaa caccgttaca ccgtcggtga ccgggcgctg gttctccggc aatcagacct
     2581 ggccgtggga tacctggaag caggcgtttg cgatggcgca tttcaatccg gacatcgcca
     2641 aagagaatat ccgcgcggtc ttctcctggc agatccagcc tggcgatagc gtgcgtccgc
     2701 aggatgtggg ctttgtcccc gacctgatag cgtggaacct tagccccgag cgtggtggcg
     2761 atggcggcaa ctggaacgaa cgtaatacca aacccagcct tgccgcctgg tcggtgatgg
     2821 aagtgtacaa cgtcacccag gataaagcct ggttggcaga gatgtactcg aaactggtgg
     2881 cctatcacga ctggtggtta cgtaaccgcg atcacaacgg caacggcgtg ccggaatatg
     2941 gcgcgacccg cgacaaagcc cacaacactg agagcggcga gatgctgttt acggtgaaaa
     3001 aaggcgataa agaagagacg cagtctgggc tgaacaacta cgcccgcgtg gtggagaaag
     3061 gcctgtacga cagtctggaa attccggcac aggttgctgc atcgtgggaa tcggggcgtg
     3121 atgatgccgc cgtctttggg tttatcgaca aagaacagct ggataaatat gtcgctaatg
     3181 gcggcaaacg tagcgactgg acggtgaaat tcgccgaaaa ccgcagtcag gacggaacgt
     3241 tgctgggcta ctcgctattg caggagtcgg tggatcaggc cagctatatg tacagcgata
     3301 accattatct ggcggagatg gcaaccatcc tcggtaagcc agaagaggcc aaacgctatc
     3361 gccagttggc acagcagctc gcggactaca tcaacacctg tatgttcgac ccgactaccc
     3421 agttctacta tgacgtacgt attgaagata aaccactggc gaacggctgc gcgggcaaac
     3481 cgattgttga gcgcggtaaa gggccggaag gctggttgcc gctgtttaac ggtgcggcaa
     3541 cgcaggccaa cgccgacgcg gtggtgaagg tgatgctcga tcctaaagag ttcaacacct
     3601 ttgtcccgct gggaacggcg gcgttaacca atccggcttt tggcgctgat atctactggc
     3661 gtgggcgcgt atgggtggat cagttctggt ttggtctgaa agggatggag cgttacggtt
     3721 atcgtgatga tgccctgaag ctggcggata cgttcttccg gcacgccaaa ggattaaccg
     3781 ccgatggccc aattcaggag aattacaacc cgctgactgg cgcacagcaa ggcgcaccaa
     3841 atttctcctg gagtgccgct catttgtata tgttgtataa cgattttttc cgtaagcagt
     3901 aatttgcaac ggctggcgga tgcggcgtga acgccttatc cgccctacat gtgcaatccc
     3961 ccaggccgga taaaacgcgg caagcgttgc atccggcgtt accaccgggc gtattctttt
     4021 tgaatcccat cacaaacccc gcactcccct tttccctttt ctccatcgac ggctaaatta
     4081 gaactcatcc gaccacataa caattatttt acatactgga caattttatg agctatccgt
     4141 cgctgttcgc cccgctggat ttaggtttta ccacgttaaa aaaccgcgtg ttgatgggct
     4201 caatgcacac cgggctggag gaatacccgg acggtgccga gcggctggca gcgttttatg
     4261 ccgaacgcgc ccgtcacggc gtggcgctga ttgtcagcgg cggcattgca ccagatttaa
     4321 caggcgttgg catggaaggt ggcgcaatgc tcaacgacgc cagccagatc ccacaccatc
     4381 gcaccattac cgaagcggta catcaggaag gcggcaaaat agccctgcaa attttgcata
     4441 ccgggcgcta cagctaccaa ccgcatctgg tcgctccctc cgcattgcag gcccccatca
     4501 accgtttcgt tcctcacgaa ctcacccatg aagagatcct gcaactgatc gacgatttcg
     4561 cccactgcgc gcaactggca cgggaggcag gatacgacgg tgtagaggta atggggtccg
     4621 aagggtattt gatcaacgaa tttctgacgc tgcgcaccaa tcagcgtagt gaccagtggg
     4681 gcggcgatta ccgcaaccgg atgcgatttg ccgtagaagt agtgcgtgcg gtgcgcgaac
     4741 gcgtcggcaa cgacttcatt attatctacc gactgtcgat gctcgacctg gtcgaagacg
     4801 gcgggacttt tgccgaaacg gtagagctgg cgcaggccat tgaagcggcg ggcgcgacca
     4861 ttatcaacac cggcattggc tggcatgaag cacgtattcc gaccattgcc acgcccgtgc
     4921 cgcgcggcgc atttagctgg gtcacgcgca aattgaaagg ccacgtctcg ctgccgctgg
     4981 taaccaccaa ccggattaac gatccgcagg ttgccgacga tattctctcg cgcggcgatg
     5041 ccgatatggt atcgatggca cgaccgtttc ttgctgatgc ggaactgctg tcaaaagcgc
     5101 aatcggggcg agccgatgag atcaacacct gcattggctg caatcaggcc tgcctcgatc
     5161 agatcttcgt tggcaaagtc acctcgtgcc tggtgaatcc tcgcgcctgc cacgaaacca
     5221 aaatgccaat ccttcctgcc gtgcagaaaa aaaatctggc ggtggtcggt gcgggacctg
     5281 ctgggctggc gtttgccatt aacgcggcgg cgcgtgggca tcaggtaaca ttgtttgatg
     5341 cccacagcga gatcggcggg cagtttaata tcgccaaaca gatccccggc aaagaggagt
     5401 tttacgaaac gctgcgctat taccaccgga tgatcgaagt gacgggcgtg acgctgaaac
     5461 tcaatcacac cgtgacggcg gatcagttac aggcgttcga tgaaacgatc ctcgccagtg
     5521 ggatcgtgcc gcgcgttccg cctatcgacg ggatcgatca tccgaaggta ttgagttatc
     5581 tcgatgtact gcgcgacaaa gcgccggtag gcaacaaggt tgccatcatc ggttgtggcg
     5641 ggattggttt tgatacggcg atgtatttaa gtcagccggg cgaatccacc agccagaata
     5701 tcgccaggtt ctgtaatgag tgggggatcg acagcagcct acaacaggct ggtggcttaa
     5761 gcccgcaggg aatgcagatc ccgcgtagcc cacggcaaat tgtgatgctc cagcgcaaag
     5821 ccagcaaacc gggacagggg ttaggcaaaa ccaccggctg gatccatcgc accaccctgc
     5881 tctcgcgggg tgtgaaaatg atcccaggcg taagttatca gaagattgac gatgacgggc
     5941 tgcatgtggt gatcaacggc gaaacgcagg tattagcagt ggacaatgtg gtgatctgcg
     6001 cagggcaaga gccaaaccgc gcgctggcgc aaccgctgat tgatagcggg aaaactgtgc
     6061 atttaattgg cggctgcgat gtggctatgg agctggacgc acgacgggca attgcccagg
     6121 gaacacggct ggcgctggag atttaaatcg ttattttaag cgccggatgc aacgcatccg
     6181 gcacgtcgcg ttaatcaata aacaaggcgg gagaaatgcc gaatcgcgtt gccaattttt
     6241 tagcgtgttc cagcgttaat ttcctcttcc cgctcaaaac gcgtgacacc atagatttac
     6301 tgccaatttc cggcagatcg gcaagggtta aaccatattg atccataagg gtacgaatca
     6361 cggctatacc gccaggcatg gcttgagcca tggcattaaa ttccgcaaat tcgggcgctg
     6421 attcttccca cgcggttatt ttggcacaca ccagatccag caaggggttt tcaggatcgt
     6481 tgagcagcag atgatctacc agttccagcg cctgggtgta ttgttcctcg ttctgaatac
     6541 ccgccagaaa aggtgccacc gcagttagct tttctcctgc ttgcaagatg tcggcaatcg
     6601 caatcatttt ttccccttag tacgatgaac agcggtaaag aaatcgtatt ctttatgcgt
     6661 cataacttca cgtatgtagc acttttgcga ttcaaaaaag accattgcta caacacgtaa
     6721 ttcattgccc ccaacattga aaacataatg cttatccaga tatttgaagt tatccagaga
     6781 tgggaatact gcttttaatg actcaggttt tttgaaatat cccttagcaa tcgtgttccc
     6841 cagagccacc aactccgttt tatgttgcgg gtatttttcc gcagcatctt tcaatgcttt
     6901 ttgagttatc aggtgcattc ttcatcacgt ccgttgacaa attggcaata tgataacatc
     6961 cgttgacaga ttggcaacgg atgaatttat ctgtgtgacg gaagaataaa cgtagaaaaa
     7021 cgctaaacaa gagaatgtag aggatggtga gagggatttt ttgttttatg gaagcgcgat
     7081 tccagaactg ggcagacatt gccggatgcg acgcttgccg cgtcttatcc ggcctgggag
     7141 taacgtgctg aacgtaggcc ggataaggcg ttaacgccgc atccggcagg taactcccaa
     7201 tcttaccgac gacgccccag cttcactgct ttcagcacca caaatttatt attcgtggcg
     7261 atggtggtgc agttgccgaa aatcttcttc agtttatgga agtaatccag gtgacggttg
     7321 gcaacaatat acagctcgcc gttgattttc aggcagcggc gggcatggtg gaacatctcc
     7381 caggcgacgt tatcggtcag cgcatgttgt tggtgaaacg gcgggttgca gagcacagca
     7441 ttaaagcgga aaggctccac gccggagagc gcgttgttga tcataaactc gctgcgatct
     7501 aacgcctctg gcatgttggt ttcaacgttc aaacggctgg aagcaaccgc catcggcgat
     7561 tcatcgacaa acaccacttt cgcctgaggg tttttatcaa gcagcgtcag accaataacg
     7621 ccattaccgc aaccgagatc gacaatctca ccttcgagat tctctggcag atgttgcata
     7681 aagaagcgtg cgccaatatc cagcccggtg cgggagaaga cattcgcatg gttgtggata
     7741 gtccagtcag taccttccag cttccagcta acggtctgcg gcgcatcggc cagcggcggt
     7801 tcattgaaag tgcaattaat caggcgcgct tttttccatg ccagcgtagt ggtggtcggg
     7861 ccgagcactt tctcgaacag ctccagcgtg gaagtgtgaa tgtcacgggc tttggcaccg
     7921 gcaataatac gtgtatccga cgtgaccact ttgcgcagcg cacgcagttg ctgttccagc
     7981 aatgccagtg ttttcggcac tttgatcagc accacacccg gctgttgcgg gtagtcggcg
     8041 gtgctgtcga gaaacttcac gctcgactca tcaatcccgt tgaggcgtaa attctcgcgc
     8101 gtcgccagtt cactgatgta tgagtcgcca atgctgtacg gcttatgttc cgccagcgcg
     8161 cagcttaacg caccaaaggc atcattcagg atcaacaccg ggccgcggat ttctgtgtcg
     8221 tccaactgtt gcagcaaata ttcatccgcc gcttcccacg cctgtagcgg gttaacgtca
     8281 tccgtcgccg gaaaacgttg tagtgtcagt gaacggaaac cgttgtctaa gtggctcatc
     8341 ggccctcctg aatgataaaa tttcgacgtt gcccctgaaa agggtgcgtg agtataccct
     8401 ttttctcttt ttcgtgggcg gttatgagca atcttactta tcttcagggt tatcccgagc
     8461 agctactttc ccaggtgcga acgctgatta acgaacagcg tctgggcgat gtgctggcaa
     8521 aacgctatcc gggaacacac gactacgcca ccgataaagc cctctggcaa tatacccagg
     8581 atctaaaaaa tcagtttctg cgcaatgccc cgccgatcac taaagtgatg tatgacaata
     8641 agatccacgt actgaaaaac gcgctcggat tacataccgc tgtttctcgt gtgcagggcg
     8701 gcaagctgaa agcaaaagca gagatccgcg tcgctactgt gtttcgcaac gcgccggaac
     8761 cgtttttacg catgatcgtg gtgcacgaac tggcgcacct gaaggagaaa gagcataaca
     8821 aagcgtttta ccagttgtgc tgccatatgg agccgcagta ccaccagctt gagttcgaca
     8881 cccggctgtg gctaacgcag ttgtcgcttg ggcaggacaa aatctgaaaa tgatgtcagg
     8941 cgttatcttt tctttcttta gcgcctaaaa tcgacctccc ccctttcgtt aaggataacg
     9001 accatgcagc cccgccttct tctccgtatt tgtttttctc gccggactct aaagattacc
     9061 tgcttactgt tacttgtggc aggagcgacc atttttatcg ccgatcgcgt aatggtcaac
     9121 gccagcaagc agctgacctg gagcgatgtc aacgcagttc cggcgcgtaa cgtgggttta
     9181 ttgctggggg cgaggccggg taatcgctac tttacccgac gtattgatac cgcagcagcg
     9241 ttgtaccacg tcggaaaagt gaaatggctg ttagtcagtg gcgataacgg acgtaaaaat
     9301 tacgatgaag cctcaggtat gcagcaggct ttgatcgcta aaggcgtacc ggcgaaagtg
     9361 atcttctgcg actatgccgg attctcaacg cttgattcag tggtgcgtgc caaaaaggtg
     9421 tttggggaaa accatatcac cattatctcg caagaatttc ataaccaacg cgccatctgg
     9481 ctggcaaaac agtacggcat cgacgctatc gggtttaacg cgccagacct taatatgaag
     9541 cacggttttt atactcaact gcgggagaaa ctcgctcgtg tcagcgcggt gattgacgcc
     9601 aaaatcctcc atcgccagcc gaaatatctc gggccatcgg tcatgattgg gccgtttagt
     9661 gagcatggct gtccggcgaa agaataatgc gtatctgcgc acgtcgaaga tgaaaaaggc
     9721 gtgctacatt gacgacagaa tccctttatg gagtatccac gcgttatgat acgtttcgct
     9781 gtgattggta cgaactggat cactcgccag ttcgtcgagg ccgcccatga gagcggtaaa
     9841 tacaagttaa ccgccgtata ttcccgcagc cttgaacagg cccagcactt cgccaatgat
     9901 ttttctgtcg agcatctgtt tacctcgctg gaagcgatgg cggaaagcga tgccattgac
     9961 gcggtgtata ttgccagccc gaatgccctg cacttttccc agacacaact tttccttagc
    10021 cataaaattc atgtgatttg cgagaaacca ctggcgtcga atctggcgga agtggatgcc
    10081 gccattgcct gtgcgcggga aaatcaggtg gtgctgtttg aggcatttaa aaccgccagc
    10141 ctgccgaact ttcatttgct gcgccaggcg ctgccgaaag tcggcaaatt gcgtaaagtc
    10201 tttttcaact attgccagta ttcctcgcgg tatcaacgtt acctggatgg tgagaatccc
    10261 aacaccttta atccggcatt ctctaacggt tcaattatgg atatcggctt ttactgcctg
    10321 gcgtcggcgg tggcattatt cggtgaaccg aaaagcgtgc aggcaaccgc cagtttgttg
    10381 gcaagcggcg tagatgccca cggcgtggtg gtgatggatt acggtgattt cagcgttacg
    10441 ttgcagcact caaaggtcag tgattctgtc ctggaaagcg agattcaggg cgaagcggga
    10501 tcgctggtga ttgaaaaact gtctgaatgc cagaaagtgt gcttcgtgcc gcgtggcagc
    10561 cagatgcagg atctcaccca gccgcagcat attaatacca tgctctacga agcagagctg
    10621 tttgctaccc tggtggatga gcatctggtg gatcatccag ggctggcggt cagtcgcatc
    10681 accgccaaac tgctgactga gatccgccgc cagactgggg tgatttttcc ggcagatagc
    10741 gtaaaactat aattgccaaa gtaaaacagt gtaaaaggta tgtaacagac cattgactgg
    10801 ctgaatggtc tgtcatactt tgttacctgc aaaggggagt aacttcattg ccggtcgatc
    10861 gtcattacga tgtgtgaaaa accacatccg gtcaccgggc aacccgaaag gaatacgcag
    10921 acgtattcct tttttgttgt aagtgagacc ttgccggaag gcgaggtcta tgcataaaaa
    10981 gcagcggctg acgtcttccg acgttggccg tttttttatg tgtaaggaac ttctatgaat
    11041 actgtcggca cgccgttgct atggggcgga ttcgctgttg ttgtcgccat tatgctggct
    11101 atcgacctgt tgttgcaggg gcgtcgtggg gcacatgcca tgaccatgaa acaggctgcg
    11161 gcctggtcgc tggtctgggt gacgctgtcg ttactgttta acgccgcttt ctggtggtat
    11221 ctggtgcaaa ctgaaggtcg cgccgttgcc gatccacagg cactggcctt tctcacgggt
    11281 tatctgattg agaaatcgct ggcggtcgat aacgtctttg tctggctgat gttgttcagc
    11341 tatttctctg ttccggcggc attacaacgc cgcgtgctgg tgtatggcgt gctcggggcg
    11401 attgttctgc gtaccatcat gatcttcact ggcagctggc taatttcgca gttcgactgg
    11461 atcctgtata tcttcggtgc cttcctgctg tttaccggcg tgaagatggc gctcgcccat
    11521 gaagatgaat caggcattgg cgacaagccg ctggtgcgct ggctacgcgg tcatttgcgc
    11581 atgaccgaca ccatcgacaa cgagcatttc tttgtgcgta agaatggatt gctgtacgcc
    11641 acaccgctga tgctggtact gattctggtg gaattgagcg acgtgatttt cgccgtggat
    11701 agcattccgg ctatcttcgc cgtgaccact gacccgttca ttgtgctgac ctcaaacctg
    11761 tttgcgatcc tcggcctgcg tgcgatgtat ttcctgctgg cgggcgtagc agagcgtttc
    11821 tcgatgctca aatatggcct ggcggtgatt ctggtgttta tcggtatcaa gatgctgatt
    11881 gtcgacttct accatattcc aatcgccgtc tcgctgggcg tggtgtttgg cattctggtg
    11941 atgacgttta ttatcaacgc ctgggtgaat tatcggcatg ataagcagcg gggagaatga
    12001 ttcgctgtat attaagccat tcctagagat aactc
//
LOCUS       DAEEKQ010000116        11928 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.117, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000116 DAEEKQ010000000
VERSION     DAEEKQ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11928
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.117"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            46..612
                     /locus_tag="KLK57_002992"
     CDS             46..612
                     /locus_tag="KLK57_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0893300.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            663..3121
                     /locus_tag="KLK57_002993"
                     /pseudo
     CDS             663..3121
                     /locus_tag="KLK57_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            3136..3867
                     /locus_tag="KLK57_002994"
     CDS             3136..3867
                     /locus_tag="KLK57_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBE0893301.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3864..4925
                     /locus_tag="KLK57_002995"
     CDS             3864..4925
                     /locus_tag="KLK57_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0893302.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLVYEKTYTAELSSIAG
                     TPIEAGPFDTVVLFKINYN"
     gene            5077..6123
                     /locus_tag="KLK57_002996"
     CDS             5077..6123
                     /locus_tag="KLK57_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HBE0893303.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6120..6911)
                     /gene="nei"
                     /locus_tag="KLK57_002997"
     CDS             complement(6120..6911)
                     /gene="nei"
                     /locus_tag="KLK57_002997"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HBE0893304.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLDIPRFSYATRGLVDE
                     NKHHGALFRFKVFHRDGEPCERCGSIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6947..7681)
                     /gene="pxpA"
                     /locus_tag="KLK57_002998"
     CDS             complement(6947..7681)
                     /gene="pxpA"
                     /locus_tag="KLK57_002998"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HBE0893305.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQIMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQRGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7671..8603)
                     /gene="pxpC"
                     /locus_tag="KLK57_002999"
     CDS             complement(7671..8603)
                     /gene="pxpC"
                     /locus_tag="KLK57_002999"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HBE0893306.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKSKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTDGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLVWRLHNEN"
     gene            complement(8597..9253)
                     /gene="pxpB"
                     /locus_tag="KLK57_003000"
     CDS             complement(8597..9253)
                     /gene="pxpB"
                     /locus_tag="KLK57_003000"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HBE0893307.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGFSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9276..10019)
                     /gene="ybgI"
                     /locus_tag="KLK57_003001"
     CDS             complement(9276..10019)
                     /gene="ybgI"
                     /locus_tag="KLK57_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HBE0893308.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDV
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10290..11771
                     /gene="dtpD"
                     /locus_tag="KLK57_003002"
     CDS             10290..11771
                     /gene="dtpD"
                     /locus_tag="KLK57_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HBE0893309.1"
                     /translation="MNKHASQPRTIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
BASE COUNT         2839 a         3183 c         2931 g         2975 t
ORIGIN      
        1 tcttgcaccg atatactttt ttcttttctg ttaaaggact cagttatgtt taaaggacaa
       61 aaaacattgg ccgcactggc cgtatctctg ctgttcacag cacctgttta tgcggctgat
      121 gaaggatccg gtgaaattca ctttaaaggt gaagttattg aagcaccgtg tgaaatacat
      181 caggatgata ttgataaaga ggttgaactc ggtcaggtga ccaccagcca cattaatcag
      241 tcacatcaca gcgatgccgt tgctgtcgac ctgcgcttag tcaactgtga tctggaaaac
      301 tccagcaacg gttccggtgg caagatttct aaagttgcag tcacctttga tagctcagcg
      361 aaaaccaccg gcgcagatcc gattctcaac aacaccagca cgggtgaagc caccggcgtc
      421 ggcgtacgtt taatgaataa agatcaaagc aatatcgttt taggcaccgc tactccagat
      481 atcgacctgg ctccgacctc cagcgaacaa acgctgaatt tctttgcctg gatggaacaa
      541 attgatcagg ctacacctgt aacgccaggc gcagttacgg cgaatgcaac ctacgtgctg
      601 gattataaat aagtattact ttggcggcgc tatcgccgcc tttcataaaa tatttatcag
      661 gtatggacac cgtgaatatt tatcgactct cttttgtatc ctgcctggtc gtggcgatgc
      721 cttgcgcatt ggcggtcgaa ttcaacctta atgttctcga taaatcgatg cgcgaccgca
      781 ttgatatttc attattaaag gaaaaaggag tcattgctcc cggtgagtat tttgttagcg
      841 ttgcggtaaa taataaccaa atcagtaacg ggcaaaagat taactggcac aaaaatgacg
      901 ataaaaccat tccgtgcatc aatgatttac tggtcgataa atttggctta aaacctgaag
      961 tccgtcagtc gttaccattg ataaatcagt gcgtcgattt tagctcccga cctgaaatgc
     1021 tcttcaattt cgatcaagcc aatcagcaac tgaatattag tattccgcaa gcctggctgg
     1081 cgtggcactc agaaaactgg accccaccct ccacatggaa agaaggtgtc gccggtatcc
     1141 tgatggatta caacttgttt gccagcagct accgcccaca ggacggcagc agcagcacta
     1201 acctgaacgc ctacggtacc accggaatta acgccggggc atggcgctta cgtagtgatt
     1261 atcagttgaa tcagactgat agcgatgata accatgaaca gtcaggcgga atatcgcgca
     1321 cctatctttt tcgtccatta ccgcaattag gctctaaatt aaccctcggc gaaacggatt
     1381 ttagttccaa tattttcgac ggtttttctt ataccggcgc ggcactggca agtgacgagc
     1441 gaatgttgcc atgggaacta cgcggctacg ccccacaaat tagcggtatt gcacagacca
     1501 atgccacggt gacgatcagt caatcaggcc gcgtcattta ccagaaaaaa gtcccaccag
     1561 gcccatttat cattgacgac cttaatcagt ctgttcaggg cacactggat gtcaaagtga
     1621 cggaagaaga tggtcgggtg aacaatttcc aggtttcggc agcatcgacg cccttcctga
     1681 ctcgtcaggg acaggttcgc tataaactgg ccgcgggtca gccacggccc tccatgtcac
     1741 atcaaactga aaatgaaacc ttttttagca atgaagtttc ctgggggatg ctgtcaaaca
     1801 cctcgctgta cggcggcctg ctgctttctg gtgatgacta ccattctgcc gcaatgggta
     1861 ttgggcaaaa tatgctgtgg cttggtgcgc tgtcgtttga tgtcacgtgg gccagtagcc
     1921 attttgatac tcagcaggac gagcggggct taagctaccg ttttaattac agcaaacaag
     1981 tggatgccac taacagcacg atttccctcg ccgcttatcg tttctccgat cgtcattttc
     2041 acagctacgc caactatctg gatcacaaat acaacgacag cgatgcgcag gacgaaaaac
     2101 agacgatcag cttattgtgg gccaaccgat taccccacta aacctcaatc tttacgccaa
     2161 cctgctacat caaacctggt ggaatgcaga cgcctccacg accgccaaca tcacggcagg
     2221 ttttaatgtt gatattggtg actggagaga tatctctatt tcgacgtcat tcaatacaac
     2281 ccattacgaa gataaagatc acgacaacca gatttacctg tcgatttcgc tccccttcgg
     2341 taacggtggt cgggttggct atgacatgca aaacagtagc cacagcacca cacaccgcat
     2401 gtcgtggaac gatacgctgg atgaacgtaa tagctggggc atgtctgccg gactgcaatc
     2461 cgaccgtcct gacaatggag cccaggtgag cggtaactat cagcacctga gttcagcggg
     2521 tgagtgggat atttctggta cctatgccgc caatgattac agttccgtca gcagcagctg
     2581 gagcggttct ttcaccgcaa cccaatatgg tgcagcgttt catcgccgca gctccaccaa
     2641 tgaaccacgc ctgatggtca gcaccgatgg cgtggcagat attccggttc agggcaatct
     2701 cgactacacc aaccattttg gcattgcggt ggtgccgttg atttccagtt accagccttc
     2761 caccgtggcg gtgaacatga atgacttacc cgacggcgta acagttgcag aaaacgttat
     2821 caaagaaacg tggattgaag gcgcgatagg ttacaaatca ctggcttccc gttccggtaa
     2881 agacgttaac gtcatcattc gcaacgccag cggtcagttt cctcccctcg gagcggatat
     2941 ccgccaggat gacagcggca ttagcgtggg gatggttggc gaggaaggac atgcctggtt
     3001 aagcggagtc gctgaaaatc aaaagtttac cgtggtctgg ggtgatagcc agcattgctc
     3061 gctccatctt cctgaacata tggaagacac cgcaaatcgc ctgattttac cttgtcatta
     3121 attaaggaaa tgaaaatgac atttatgaaa ggactacccc ttttgttgtt ggttgccagt
     3181 ctgtgcagcc acgctgcact acaacccgat cgcactcgta ttgtgtttaa cgctaatgat
     3241 aaagctacca gtctgcgggt ggataatcgt agcgataaac tcccctacct cgcttattcc
     3301 tggctcgaaa atgaaaaagg ggagaaaagc gatgatcttc tggtcgccct gccgccgata
     3361 caacgtctgg aaccgaaagc aacaacgcag gtacggatag taaagcaagc ctcgaccaca
     3421 aaattaccgg gcgatcgcga aacgctattt ttctacaata tgcgtgaaat tcctcccgcc
     3481 ccggaaaaaa acagcgacca tgccgttctg caagtcgcca ttcagagtcg tatcaaggtg
     3541 ttctggcgac cggctgcatt acgcaagaaa gcaggagaaa aggtcgaact gcagttacag
     3601 gtcagccagc agggcaacca actgacactg aaaaatccta ccgcgtatta tctgaccatt
     3661 gcctacctgg gacgaaatga aaaaggcgtt ctccccggct tcaaaaccgt gatggttgcg
     3721 ccttttagta cggtcaacac aaacaccgga aattatagcg gcagccagtt ttatctcggt
     3781 tatatggatg attacggcgc gttacgcatg acgacgctca actgcagcgg acaatgccat
     3841 ttacaagccg tggaggcgaa gaaatgagtg ctggcaaggg agtgttgctc gtcatctgtc
     3901 tgttatttct gccgttgaag tctgcgcttg ccctgaactg ctactttggt tcatccggtg
     3961 gctcggtaga aaaatcagaa gcgattcaac cttttgctgt accaggcaat gccaaactcg
     4021 gcgataagat ttgggaatct gacgatatta aaattcccgt ctattgtgac aacaatacca
     4081 acggcaattt tgaaagtgag catgtttatg cctgggtaaa cccctacccg ggggtgcagg
     4141 atcgctatta tcaactcggc gtaacttaca atggcgttga ttatgacgcc aaccagggaa
     4201 aaagccgcat cgacactaac cagtgtattg acagtaagaa cattgacatt tatacccctg
     4261 agcagattat cgcgatggga tggcagaaca aaatttgctc gggtgatccc gctaatattc
     4321 acatgtcgcg tacctttctc gctcgcatgc ggttatacgt caaaatacga gaaatgccgc
     4381 cgcatgatta tcaaagtacg cttagcgact atatcgtcgt gcaatttgac ggtgccggta
     4441 gcgttaacga agaccctact gcccaaaacc tgaaatatca tattactggt ctggaaaaca
     4501 ttcgcgtgct ggattgcagc gtcaattttt ccatttcacc ggaaacacag gtgattgatt
     4561 ttggtaaatt taatttgctg gatattcgtc gccacacaat gtcgaaaaca ttcagtatta
     4621 agacgacaaa atcacaaaat gaccaatgca ccgacggatt taaagtcagt tcctcctttt
     4681 ataccgaaga aacgttggtt gaagaagata aagcattgtt gattggtaat ggtctgaagc
     4741 tacgtttgct ggatgaaaac gcctcgcctt ataccttcaa taaatatgct gaatacgccg
     4801 atttcaccag cgacatgttg gtctacgaaa aaacctatac agctgaactt tcgtccattg
     4861 caggtacccc catcgaggct gggcccttcg atacagtggt gctttttaaa attaactata
     4921 actgatttgc catgatgccc tttgcagccg gaaagggcat ctctttcaga aaattgctga
     4981 ttttcccctt ttccttgatt gctgttattt tttcctggcc cggctttctt tatgctgtat
     5041 cgatacacca aagagaaaat aatgagggag cgaaggatgc cagttttgca gtggggaatg
     5101 ttatgtgtgc tatcacttct cctttctatt ggtttcctcg cggtacatct cccggcggcg
     5161 ctattacttg ggccgatgat cgccgggatc atcttcagta tgcgcggaat tactctgcaa
     5221 cttccccgct ccgcttttct cgccgcgcag gccattcttg gctgcatgat tgcgcaaaac
     5281 ctcactggtt cgattctcac cacgctggcc gccaactggc cgatcgtgct tgctatttta
     5341 ctggtgacgc tgctttccag cgccatcgtg ggttggttat tggtgcgcta tagctcactg
     5401 cctggaaata ccggtgcctg gggttcttct ccaggcggcg cagcggcaat ggtcgctatg
     5461 gcgcaagatt acggcgcaga tattcgcctg gttgcattta tgcaatatct gcgagtgctg
     5521 ttcgtcgcgg gggccgctgt tctggtcacc cgcatgatgc tgggtgataa cgctgaagcg
     5581 gttaaccagc aaatcgtctg gttcccgcca gtgagcatta atctcctgct tacgattttg
     5641 ctcgcagtcg ttgccggtac ggcgggatgc atgctgcgtc ttccttccgg cacgatgctc
     5701 atcccgatgc tggcgggcgc agtgctccag tctggtcagc tcatcaccat cgaactaccg
     5761 gaatggctgc tggcgatggc gtatatggca attggctggc ggattggtct tggtttcgat
     5821 aaacaaatct tactgcgggc actacgcccg ctaccgcaaa tcctgctgtc gatttttgct
     5881 ctgctggcta tttgtgcggg tatggcgtgg gggctgaccc ggtttatgca cattgatttt
     5941 atgactgcct acctcgccac cagtcccggc gggcttgata ccgtggcggt catcgccgca
     6001 gggagcaatg ccgatatggc gcttattatg gcgatgcaaa ccctgcgcct gttcagtatt
     6061 ttgctgacgg ggccagccat tgcgcggttt atttcaacct atgcgccgaa gcggtcggcc
     6121 tagtgctggc agccagggca ccagtaaaac gggcgagatg acagcgtggt tttctcaatg
     6181 atgctgccac agcgttcgca cggttcgcca tcacgatgaa aaaccttaaa gcgaaacagc
     6241 gccccatgat gcttattctc atccaccagc cccctcgtag cgtaggaaaa tcgaggaata
     6301 tccagtaacg cgtgtgcgag tgcatccagt tgcgccgcat tgagatcttt cgctttatga
     6361 tttccagtca acccaacctg ccagaggatc tccacccgca aataattgcc aagcccagcc
     6421 agaaacgcct gatcgagcag taatcctgca aactgacggt tacgaaagcg cggtgacaat
     6481 aaccgttcct ttaccacctc cggcgtcaga ttcggatcca gcacatcggg accaacgcgt
     6541 tgtaaaaacg gatgcgtggt cagttgttcc ggcctcaaca tctcaatatc cgaggcgcta
     6601 taaagcagaa tggttttgtc agccgtttgc agttttaccc gcaatactcg cgtggtctgc
     6661 ggctcttcgc cggtatcaac cacgcgccag acgccgtaaa gctgattatg gctgtagagc
     6721 gttaagtcgt tggaaaaatg agttaacaac gccttaccac gcgtttccac atgggtaacg
     6781 tgttgaccga taagttgtga ttgataagtt tttaactgcg ggaaggcaaa ccagacatca
     6841 gttagtggtt tgcctttgat cgccgcctcc aggttatccg ctgcacggcg gatctccggg
     6901 ccttcaggca tggtgatatc cttttttcat tctttattaa tcggggttat gctgcgacaa
     6961 caatctcctt ttcggcaaat gtagagcgta gtcggcgggc gaacgccagt gcgtgctcgc
     7021 cgtcgccatg caggcagact gtttgcgccg tgaccgttgc ccattcgccg gtgatacttt
     7081 tgactctgcc gtgttgcacc atctctagcg tttgcgccaa cgcctgttct tcgttttcaa
     7141 tcaacgcgcc tcgctgactt cgcggcacca gcgagccgtc agcctgataa ccgcgatcgg
     7201 caaacacttc ctcgcgcgtt gtcaggccat attgcttgcc tgcacgaatc agctcgcttc
     7261 ctgccagccc aacgagaatc aatgccggat cgcaagcgta taccgctctg gcgatggcgt
     7321 ctgccagttg tgcttctttc gccgcctggt tgtacaacat gccgtgcggt ttgacatgac
     7381 gcattacgcc gccttgcgca cgggcaatcg ttgccagcgc gccaatttga tacagcgtct
     7441 gggcgtaaac ggtttctggc ggcagctgca tggcgctgcg accaaaattt tccctgtcgg
     7501 gaaaactcgg gtgagcgcca atcgcgacac cattttttat tgcttcacgc acgcaagcct
     7561 gcatgatttg cgcatcgcct gcatgaaatc cacaggcaat attggcagag gaaaccagcg
     7621 ttaatagctc tgcgtcgctg gcgcagcctt cgcccagatc ggcgttcagg tcaattttca
     7681 ttgtgcagcc gccacactaa ttgttcgaaa taacgttgct gatcttgccg cgctttcagt
     7741 gcctcttcca gtgaacactg gacaaaatga atcggctgac cgagcggaat ttgcgccaga
     7801 tggtacatat cagcctcaat gatacaggca atacgcgggt aaccatcggt ggtctgtgcg
     7861 tcgttcatca acacaatcgg ctgcccgtta tgtggcacct gcaccacgcc cggtaacaaa
     7921 ccgtgagata acagttcgcg atcggtggtg cgttttaaaa tttgcccctg taagcgatag
     7981 cccatgcggt tactttgcga gctaagctgc cagggcgaac gccagaatgc atcctgcgag
     8041 gcgcgatcga actcatgata ttccggcccc ggcaaggcgc gaatgcggtt gccccacagc
     8101 agctgtttaa cgccctgcgc ttccataaaa tcacgcttcg atttgccaat cgggagtcgg
     8161 tcaccatcct tcagtaaacg gccttccagc ccgccaatcc ccactttgag atcggtgctg
     8221 catgacccca ttaccggcgg aacatcaata ccacccgcga ccgccagata actgcgcatc
     8281 ccgtgctgcg ggcgtttaag cgttaaacgc tggcctgctt tcatcggcaa tcgccagccg
     8341 gtccagacgg cattatcatc cagccgcgct tcgcaaccgg cacccgtcag agcaaaccac
     8401 ccatcagttt cgaactcaac cgttaactga ccgagcgtga tctccagcgc gggggcattg
     8461 gcgtcattac ccaccagcag gttagcaatg cgtaacgcag gcatatccag tgcgccgcag
     8521 tggctgattc ccgactggcg aaaaccgtga cgaccgccat cctgcacagt ggtatacatg
     8581 cccgcacgaa taatcttcag catactccct ccttctgcgg tacaaagcgc acgctgtctc
     8641 ccggacgtaa taagatgggt tcgtcacgcg ccggatcaaa caggctgagt gaggtatgac
     8701 caatcaactg ccagccaccc ggcgttgcca gcggataaac accagtctgc ggcccgccga
     8761 tcccgacaga acctgccgga acgagcaagc gcggttcagc gcgccgtggc gtgtgtagtt
     8821 gttccggcaa actcccgaga tacgggaagc ccggttgaaa acctaaaaac cagaccacgt
     8881 attccacgga ggagtgcaat tcaacaacct gtttttcgct aaacccgcaa tgcgccgcga
     8941 ccaccgccaa atccggtccg cctgcaccac cgtaaaccac cggaatttca ataaagcgag
     9001 actcaggttc cagggcctcg ctctcctccc accagcgttg caaacgctct atggcatcca
     9061 gcgccagcga ctcaggatta cgcaaaatca ccgtgatatt gttcatgccg ggaatggctt
     9121 caaccacatt cggcatatcc accagacgct gcgccagtcg ccagatccgt ttctggctag
     9181 ccagcgtcac cggcggttcc agttccagca ctaccgccgt ttcacctatc agataacaac
     9241 gcgctcgttg cactttcgat ccctctgatt attcgttatg caggattagg aatatcaata
     9301 aaggtcacat caagatcggt attttcattc agccactcgc tcaatgcgcg aataccacca
     9361 cgttcagtgg catggtgacc tgcagcataa aaatgcaacc cctgctcgcg ggctgaatga
     9421 atggtctgtt cagaaacttc gccagtaata aaagcatcca cgccaaaacg cgcggcgcta
     9481 tcgataaaac tttgcccgcc gcccgtgcac caggcgacgc gctggaccac ctcaggtccg
     9541 gtatcgccac accataacgg cttacgtccc agacgcgctt caatccagga ggctagttcc
     9601 agtcccggca caggcatggt cagctctccc cacggcacca acggctcaat ttcgcccatg
     9661 accgtgatcc ccagtaacgc cgccagctgt gcgttattgc ccagctcagg atgtacgtca
     9721 agcggcaaat gccagccata caggttgata tcattcgcca gcaacgtttt taaacggtta
     9781 cgcttcattc cgcgaatgac cggagactct cctttccaga agtagccatg atgcacaatg
     9841 accgcatccg cgcccagacg cactgcctca tcgagcaaag cctggctggc ggtgacaccg
     9901 gtaacaattt tttgcaccgt ctctttgcct tctacctgca aaccattcgg cgcgtaatca
     9961 ctaatcgccg cgctgttcag tttttcgtta atcagttgtt ccagttcggt gtttttcatc
    10021 ttcgcgcctt ccgtattctg atagctacac cgttaacata gcgttacctc gcctgttcgt
    10081 cgatagccaa tcttttgaca tattaagaaa caaacccgac gccaaaagac gcaaattgtt
    10141 tatatcatca cgcttcagct aatgtatatt tatgcaatca gacagcatat tgcataaata
    10201 atcagatctg tatcaaagtt ttaactttaa ggaaacacaa gcacgccgcg caactattac
    10261 gtgacgttgc agtcaattgt ggaattatta tgaataaaca cgcatcacaa ccgcgcacta
    10321 tttactatgt cgttgcgctg caaatctggg aatattttag cttttacggc atgcgtgccc
    10381 tgctgattct ctatctcacc aatcaactaa aatacaacga tactcacgcc tacgagttat
    10441 ttagcgccta ctgttcgctg gtgtatgtca cgccaatcct cggtggcttt ttggcggata
    10501 aagttctcgg caatcgcatg gcggtgatgc tgggggcgtt gttgatggcg atcggtcatg
    10561 tggtgctggg tgccagtgag atccatccgt cattcctcta tctgtccctg gcgattatcg
    10621 tttgtggcta tggtctgttt aaatccaatg tcagttgcct gctcggcgag ctgtatgagc
    10681 caaccgatcc gcgtcgtgat ggcggtttct cgctgatgta tgcagcgggt aacgtggggt
    10741 ctattatcgc acctatcgcc tgtggttacg cccaggaaga gtacagctgg gcgatgggct
    10801 ttggcctggc ggcggtgggt atgatcgcgg gtctggtcat tttcttatgt ggcaatcgtc
    10861 atttcacaca tacccgcggc gttaataaaa aagtactgcg tgcaacaaac tttctcctgc
    10921 ctaactgggg atggctgctg gttctgctgg tggcaacgcc tgcgctgatt accgtactgt
    10981 tctggaaaga gtggtcggta tacgccttaa ttgtcgcgac tattattggc ctgggtgtac
    11041 tggcaaaaat ttatcgcaaa gcagaaaacc agaaacagcg taaggagctg gggctgattg
    11101 tgacgctcac cttcttcagt atgttgttct gggccttcgc acaacagggc ggcagctcaa
    11161 ttagccttta tatcgaccgc ttcgttaacc gcgatatgtt tggttatacc gttccgactg
    11221 ctatgttcca gtcgattaat gccttcgcag ttatgctatg cggtgtgttc ctggcgtggg
    11281 tggttaaaga aagcgtcgca ggtaatcgta ccgtgcgcat ctggggaaaa tttgctcttg
    11341 gccttggcct gatgagcgcc ggattctgca ttctgacctt aagcgcccgc tggtccgcaa
    11401 tgtatggtca ctcttctctg ccactgatgg tgttaggcct cgcggtgatg ggctttgcgg
    11461 aactgtttat cgacccggtt gccatgtcgc aaattacgcg cattgaaatt ccgggcgtga
    11521 ccggcgtatt aaccggcatc tacatgctgc tttctggcgc gatcgcgaac tatctggcgg
    11581 gcgtgattgc cgatcagaca tcgcaggctt cgtttgatgc ttccggggcg atcaactact
    11641 ccatcaatgc atatattgaa gtatttgatc aaattacctg gggcgcactg gcgtgtgtag
    11701 gactggtact gatgatttgg ctgtatcagg cgctgaaatt cagaaaccgc gcgctggcgc
    11761 tggagtcata ataggccagc accgatgcct gatgcgacgc ttgacgcgtc ttatcaggcc
    11821 ggtgatgctg ccaacttact gatttagtgt atgatggtgt ttttgaggtg ctccagtggc
    11881 ttctgtttct atcagctgtc cctcctgttc agctactgac gggggggt
//
LOCUS       DAEEKQ010000117        11904 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.118, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000117 DAEEKQ010000000
VERSION     DAEEKQ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11904
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.118"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(145..408)
                     /gene="cedA"
                     /locus_tag="KLK57_003003"
     CDS             complement(145..408)
                     /gene="cedA"
                     /locus_tag="KLK57_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HBE0893310.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            591..2852
                     /gene="katE"
                     /locus_tag="KLK57_003004"
     CDS             591..2852
                     /gene="katE"
                     /locus_tag="KLK57_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HBE0893311.1"
                     /translation="MSQHNEKNLHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(3110..3868)
                     /gene="chbG"
                     /locus_tag="KLK57_003005"
     CDS             complement(3110..3868)
                     /gene="chbG"
                     /locus_tag="KLK57_003005"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HBE0893312.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMYFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNSG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELDVLTSASLKGAIAQRGYRLGSYRDV"
     gene            complement(3881..5233)
                     /gene="celF"
                     /locus_tag="KLK57_003006"
                     /gene_synonym="chbF"
     CDS             complement(3881..5233)
                     /gene="celF"
                     /locus_tag="KLK57_003006"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBE0893313.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5338..6180)
                     /gene="chbR"
                     /locus_tag="KLK57_003007"
     CDS             complement(5338..6180)
                     /gene="chbR"
                     /locus_tag="KLK57_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HBE0893314.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(6188..6538)
                     /gene="chbA"
                     /locus_tag="KLK57_003008"
     CDS             complement(6188..6538)
                     /gene="chbA"
                     /locus_tag="KLK57_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HBE0893315.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6589..7947)
                     /gene="chbC"
                     /locus_tag="KLK57_003009"
     CDS             complement(6589..7947)
                     /gene="chbC"
                     /locus_tag="KLK57_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HBE0893316.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGAFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYVVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEEREEDIANAL
                     KF"
     gene            complement(8032..8352)
                     /gene="chbB"
                     /locus_tag="KLK57_003010"
     CDS             complement(8032..8352)
                     /gene="chbB"
                     /locus_tag="KLK57_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HBE0893317.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(8651..8989)
                     /gene="osmE"
                     /locus_tag="KLK57_003011"
     CDS             complement(8651..8989)
                     /gene="osmE"
                     /locus_tag="KLK57_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HBE0893318.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            9191..10018
                     /gene="nadE"
                     /locus_tag="KLK57_003012"
     CDS             9191..10018
                     /gene="nadE"
                     /locus_tag="KLK57_003012"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HBE0893319.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSIDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRLETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            10248..11135
                     /gene="cho"
                     /locus_tag="KLK57_003013"
     CDS             10248..11135
                     /gene="cho"
                     /locus_tag="KLK57_003013"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HBE0893320.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQTIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(11095..11670)
                     /gene="ves"
                     /locus_tag="KLK57_003014"
     CDS             complement(11095..11670)
                     /gene="ves"
                     /locus_tag="KLK57_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HBE0893321.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFAADQVVKAKLTEGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
BASE COUNT         3157 a         3049 c         2715 g         2983 t
ORIGIN      
        1 acaattaaag aaaaaagatc acttatttat agcaatagct cgtcaaaggc agctttttgt
       61 tacaggtggt ttgaatgaat gtagcaacga aatgcagaat ttcaggtcat gtaactcccg
      121 gcaaaaccgg gaagtatgta atccttactc agtcacttcc ccttcctggc ggatctgatt
      181 tgcccaacgt tgggcagatt caggcacagt aaacgccggt gagcgcagaa atgactctcc
      241 catcagtaca aacgcaacat atttgccacg cagcatccag acatcacgaa acgaatccat
      301 ctttatcgca tgttctggcg gcgcgggttc cgtgcgtggg acatagctaa taatctggcg
      361 gttttgctgg cggagcggtt tcttcattac tggcttcact aaacgcatat taaaaattag
      421 aaaaactgta gtttagccga tttagcccct gtacgtcccg ctttgcgtgt atttcataac
      481 accgtttcca gaatagtctc cgaagcggga tctggctggt ggtctatagt tagagagttt
      541 tttgaccaaa acagcggccc tttcagtaat aaattaagga gacgagttca atgtcgcaac
      601 ataacgaaaa gaacctacat cagcaccagt caccactaca cgattccagc gaagcgaaac
      661 cggggatgga ctcactggca cctgaggacg gatctcatcg tccagcggct gaaccaacac
      721 cgccaggtgc acaacctacc gcccctggga gcctgaaagc ccctgataca cgtaacgaaa
      781 aacttaattc tctggaagac gtacgcaaag gcagtgaaaa ttatgcgctg accactaatc
      841 agggcgtgcg catcgccgat gatcaaaact cactgcgtgc cggtaaccgt ggtccaacgc
      901 tgctggaaga ttttattctg cgcgagaaaa tcacccactt tgaccatgag cgcatcccgg
      961 aacgtattgt tcatgcacgc ggatcagcag ctcacggtta tttccagcca tataaaagct
     1021 taagcgatat caccaaagcg gatttcctct cagatccgaa caaaatcacc ccagtatttg
     1081 ttaggttctc taccgttcag ggtggcgctg gctctgcaga taccgtgcgt gatatccgtg
     1141 gttttgccac caagttctat actgaagagg gtatttttga cctcgttggc aataacacgc
     1201 caatcttctt tatccaggat gcgcataaat tccccgattt tgttcatgcg gtaaaaccag
     1261 aaccgcactg ggcaattcca caagggcaaa gtgcccacga tactttctgg gattatgttt
     1321 ctctgcaacc tgaaactctg cacaacgtga tgtgggcgat gtcggatcgc ggcatccccc
     1381 gcagttaccg caccatggaa ggcttcggta ttcacacctt ccgcctgatt aatgccgaag
     1441 ggaaggcaac gtttgtacgt ttccactgga aaccactggc aggtaaagcc tcactcgttt
     1501 gggatgaagc acaaaaactc accggacgtg acccggactt ccaccgccgc gagttgtggg
     1561 aagccattga agcaggcgat tttccggaat acgaactggg cttccagttg attcctgaag
     1621 aagatgaatt caagttcgac ttcgatcttc tcgatccaac caaactgatc ccggaagaac
     1681 tggtgcccgt tcagcgtgtc ggcaaaatgg tgctcaatcg caatccggat aacttctttg
     1741 ccgaaaacga acaagcagca ttccatccag gtcatattgt tcctggtctg gacttcacca
     1801 acgatccgct gttgcaggga cgtttgttct cctataccga tacacaaatc agtcgtcttg
     1861 gtgggccgaa tttccatgag attccgatta accgtccgac ctgcccttac cacaacttcc
     1921 agcgtgatgg catgcatcgt atggggatcg acactaaccc ggcgaattac gaaccgaact
     1981 cgatcaacga taactggccg cgcgaaacac cgccgggacc gaaacgcggc ggttttgaat
     2041 catatcagga acgcgtggaa ggaaataaag ttcgtgagcg cagtccatcg tttggcgaat
     2101 attattccca tccgcgtctg ttctggctaa gtcagacgcc attcgagcag cgccatattg
     2161 tcgatggttt cagttttgag ttaagcaaag tcgttcgtcc gtatattcgt gagcgcgttg
     2221 ttgaccagct ggcgcatatt gatctcactc tggcccaggc ggtggcgaaa aatctcggta
     2281 tcgaactgac tgacgaccag ctgaatatca ccccacctcc ggacgtcaac ggtctgaaaa
     2341 aggatccatc cttaagtttg tacgccattc ctgacggtga tgtgaaaggt cgcgtggtag
     2401 cgattttact taatgatgaa gtgagatcgg cagaccttct ggccattctc aaggccctga
     2461 aggccaaagg cgttcatgcc aaactgctct actcccgaat gggtgaagtg actgcggatg
     2521 acggtacggt gttgcctata gccgctacct ttgccggtgc accttcgctg acggtcgatg
     2581 cggtcattgt cccttgcggc aatatcgcgg atatcgctga caacggcgat gccaactact
     2641 acctgatgga agcctacaaa caccttaaac cgattgcgct ggcgggagac gcgcgcaagt
     2701 ttaaagcaac aatcaaggtc gctgaccagg gtgaagaagg gattgtggaa gctgacagcg
     2761 ctgacggtag ttttatggat gaactgctaa cgctgatggc agcacaccgc gtgtggtcac
     2821 gcattcctaa gattgacaaa attcctgcct gatgggaggc ggcgcaattg cgccgcctca
     2881 aatgatttac atagtgcgct ttgtttatgc cggatgcggc gtgaacgcct tatccggcct
     2941 acaaaactgt gcaaattcaa tatattgcag gaaacacgta ggcctgataa gcgaagcgca
     3001 tcaggcagtt ttgcgtttgt cagcagtctc aagcggcgca gttacgccgc ctttgtagga
     3061 attaatcgcc ggatgcaagg ttcacgccgc atctggcaaa catcctcact tacacatccc
     3121 gataactccc caaccgataa ccacgctgag cgatagcacc tttcaacgac gctgatgtca
     3181 acacatccag ctccgttaag cgtgggaaac agtaagcact ctgacggata gtattatcga
     3241 taaacgccgg atgacacatc acctccagcg aacgatcacc ccgatggctt gcgtcatcca
     3301 gcacttgcag gaacagtgcc tcgctaatct cttcaccata aaacgcgcta ctgaatccct
     3361 gagaactgcg cagattagcc ggtaaatcac cagagttaga tagcggctga cggtcgatgc
     3421 gcaacgcaat cccctcttca gccgcaaacc tggcaacaat cgggaaaatc tgcgggaaca
     3481 tatgcacatg atgatggcta tcaagatgcg taggtttgcg tccaaatagc tcaatgaaac
     3541 gcaaatactg actggcaagc tcctgagtaa tttcttccag cggtaaagca tcttcttctg
     3601 ccaactgcca gatccatttt cccagcacac catcgcgggt taaccccggc atagctgtca
     3661 gtggcttgcc catagtaagg acaaagtaca tccctatggc cagactcggt tcatcacgac
     3721 tcaactgcac cgcatggtca atagcctgcc cattcaccag cgccgtcgtc gatgtgacaa
     3781 tcccattgcg acaggcctcg ataatgccgt agttctgacc tttgcttaag ccaaaatcat
     3841 cggcattaac aatcagtaag cgttccataa tcagcctcgg ttaatgtgct tttttaagct
     3901 ctgcgatgca gtcggcaaaa tttggcagcc atttctcgtg cgccagaatc atctcgcgtg
     3961 ccagcagctc agcatcgcga tcggaatgca ccaacggact aaggtttagc gccagtaaca
     4021 catcgttaaa ttctccgcta agtgcggcgt tgctggcagc aatctcgaag cctttaatgg
     4081 tgtgaatcag ccccatcact ttatcatcga aatgcgtaat gcgcggatgt ggcgtcgcgc
     4141 catcgcgccc cagcttacag gtcatttcta ccgcccagtc tgccggaata ttatcaatat
     4201 gcccatgatg cgggatatta acgtaatgtt cagcttgctt gtcgttgtag atagcgttga
     4261 tcacttcgca cgctgcatca gagtaataag ccccaccgcg ctgttccagt tctttcggct
     4321 taactttcaa ctccgggttt ttatacagct caaaaagttg tttctcgact ttctgtacta
     4381 cctgcgctcg tgcgccgcct ttgtagtatt cgcccatttc aatagccagc atctcttttt
     4441 gcttgaagta ataaagcaaa taagaacacg gcaacagatt cagagaacga attaagcctt
     4501 cgctaaatgg cagatcgaaa atatttttaa cgccagatgc ttttaactgc ccggatgcca
     4561 caccatcaag caattcggca aagcgcgact tgccatttac cagcacatcc ttaatgaaca
     4621 ccatatggtt gaggccgaac aaatcgatag ataaatcatc gctgtctttc agcatcagaa
     4681 catcgtgaat aaacatcttc atgccgatcg gaatattaca cacgccgata aagcgtttaa
     4741 atccggtatg acgataaacg gcttcagtaa ccattcccgc cgggttagtg aagttaatca
     4801 cccatgcatt cggacaaagt tcttcgacat cttttacgat gtcaaaaatc accggaatgg
     4861 tacgcagacc tttaaacaga ccgcccgcgc cgttggtttc ctggccaaga taaccatgac
     4921 ttaatggaat acgttcatcc agttcgcgcg ccggtaattg gcctacgcgc agttgggtag
     4981 taacgaaatc agcatctttc aatgcttcgc ggcgatccag cgttttataa agcttcatcg
     5041 ggacgccagc gttatcaatc atccgttggc agagttcaaa aatgatatcc agtttctctt
     5101 taccatcttc gacatccacc agccataatt cgctgaccgg caattcgtga taacgcttaa
     5161 taaatccttc cagtaactcc ggggtatagc tgctcccgcc accaatagtg acgactttta
     5221 atttctggct cataatttct cccttcagta cagaatactg atatctggca tatctgcccc
     5281 cccggacata aataatccag caacaggaca gatatgtgaa ttgtcaggta taacgactta
     5341 ctgattaaat tcagtcaatt tcttacgata gctcttaggc gtaaaggacg ttaatttctt
     5401 aaacgtttta ataaacaaac tcgggctact ataaccggcc tcaaacgcaa tatccgtcac
     5461 ggaatagttg gtcatttcca gttgtttttt ggcaaaatta atacggatct cattaataat
     5521 ctgcattggt gttttgccat aatatcgttg agtcgctcgc gtcaaatatt cctgtgattt
     5581 ggctgacaac gtcaccatat tctccagcgc cgattcacta aactgctctt tatcatgcat
     5641 cttttctacc gtacttttca gccactgcgg tacatcatca atcacctgtt cttcgcggta
     5701 atgacgtaaa cggttaatga cataaaaagt aaccatctca acaaactctt ccagccctgt
     5761 ttcgcggaaa ttcaatgaag aaatcactgt ttccacatag gtgagaaaag cgttattggt
     5821 ccggtatacc tgcgaagcga caaagcaata aggcaacaac ggcaggtaat gctgctcaaa
     5881 aaagcgttta ctgatcccaa cgttcaatat acgcgtggca ccaaactcat aaaaactttg
     5941 atggtgcgaa cctaacggaa taaaaacaaa atcgccccgt tccagtaaca cgcgcttacc
     6001 gttaatctcc tggaaataac gcccggttaa taccagagta aattcataat agtcgtgctg
     6061 atgcagcccg ctgatactct cagttttgtt atagataaac acatggaagt ttttgccatt
     6121 aaacaactgc tgttctcggg cagtggcaat ttccggcgcg ttaatcactg gctgcatcat
     6181 cgactcctta tgccttcagt ttttcatgaa gctcaattaa ttcagtaatc agttcacgcg
     6241 caagcatgga cgtcattaaa tgatcctgag cgtggaccag caccagactc actttcatct
     6301 taccttcgcc cgcatcgcct tcaatcagtt tcgtctgtac cagatgcgct tcattcaatg
     6361 ccatccgtga ctgatccatc atggcttttg ctgcagcaaa atcgccctgc ttcgcctgtt
     6421 tcagtgccgc atacgccagg ctgcgcgctt gtccggagtt gatgataagc cccatcacca
     6481 cttcttccag ctcttcagct tccgtttgcg tatcgggaat gttatcgaga tccatcatac
     6541 atcgttcctc tctattctta ccggcacgat tacccgtacc ggcatcgatt aaaatttcag
     6601 ggcgttagcg atatcttctt cgcgctcttc tttatcaatc gcgttttgtg ctttgttagc
     6661 caccacaaca aagggcagat aaattaacgt tgcgatacca aggttgaaga gtgcaaccag
     6721 caatgcggcg acgctaccgt tggtgttaaa gaaggctccc agaccggttg gcatggtcca
     6781 cggtgcaata ttggtcaccg gaggaatgat gcccatgtag tacgctgcga gggtgattgc
     6841 cgccagaatc ggttgtacca gtacaaacgg gataaacatc accgggttca tgataattgg
     6901 cagaccaaac agaatcggtt cgttaatctg gaagatgccg gacggcagcg ccagttttgc
     6961 cacctgacga taatcagcac ggcgagaggc gataaagata gccaggatca ggcctaaagt
     7021 cgcaccactg cccccaagga aaataaagga gtccagcatc ggcttggccc agatatggaa
     7081 ggtcttaccg gctgccagcg ccgcttcgac ggaaccatat tgctgatagg tcgcgatatt
     7141 ttccagcgcc cacggcgtca taatgccgtt gtccagtgcg gtcagcgcca gcgcgccatg
     7201 aataccgaag aaccagagca gtggaacaaa gatcacatag gcccagccca ccacgctacc
     7261 caacgatgcc agtggggttg agatggtatc cataatgatc tgatggaagt tggtgcccca
     7321 ggtattcaac gcccaggcaa taatccccat cacggaaaga ataataaagc cgggaattaa
     7381 tgccgagaag gaacgcgata ctgaagcagg tacgctatcg ggcaatttaa tgacccaatt
     7441 gcggcggaca ataaaggtaa acatttctgc caccaccagg ccaataataa tcccggagat
     7501 gatattcgct ccacctaacc agtttgcgcc aaccacatag gcctcaccga cactatatgg
     7561 ggtgacggtc ataaatgctg caacggataa caacccagcc gccagcgcat cgactttacg
     7621 ctcttctgcc agcgccatgc caataaagaa cggtgccatt aaagacatta tccctaatgt
     7681 tccgttatat acgttgccgc caataccttt caggccatta agtgtttcaa tggttgaggc
     7741 gtcgaggcga atacctaagg aataaaaaaa cgccccctcc ccaaagctta gaaaaacgtt
     7801 gttaattaat acaaacatgg cccccgcaag ggttaacggc attaagcgaa taaagccatt
     7861 tttgattgca ttaacgtgtg gctgctttcc tattttaact gcaaaaggga ggagtacctt
     7921 ttcaagcgat gcaataacat tactcataga aaaataccct taaaaaccgc aatttaaata
     7981 ttgcggtatt gatttatgaa ataactcttt gacgggaaaa tttaaaataa attaatttgc
     8041 tgcggctttt ttaatcgctg caaccgcagc cttaagcacg cctaaaccat cgactttgcc
     8101 ataaagcagc gagtcaatta cttcaaccgg tttgttgggt aacaaacgct ggatttcggg
     8161 caacatataa gcaatctgcg gccctaataa cacgacatcg gcattctgac ctttttcacc
     8221 agccagtgtt tccggaaatg cttcaataat gaccggaact tcatattttt ctgcctgtgc
     8281 gcgcattttt gataccagta aagaggtaga catgcccgca gaacaaaaca gataaatgtg
     8341 tttcttttcc ataaaactgc cctcatcgac gattatctgt cagccagaca ctccgcaagc
     8401 cttaacctgc ttccatgctc tgggtaactt gcgaaaccaa catgactttt tttgtggctg
     8461 aaaggagtat acggtataga cctgagcaat agtatttctt tgaccttata aattgtttct
     8521 ccttgacctg tggttatgac cctcttcaca tttcgggcaa ataattcgcg aagataatta
     8581 agaactcagg ccaaaaaaaa ccggcgcaat ggccggtttc cgttgttact caagctttca
     8641 gacgaattga ttacttcgca gcctgtggat cagtgtcgta ttcagcacag gtctgataac
     8701 cggagttgat gacatgtccg gtatcatcta acgcgacaaa gtaggtttct gctttaccat
     8761 cacgttgacc caggatgtag gtctggcaag taccacgagc atggatcatg ctcacttcag
     8821 acgaaggttt acccgcaatt tgtgcaacct gcgcccggct catgcctttt ttgacgtctt
     8881 tcaccacagg ctgtacaaac tggtctttag tacgatcata agccgtacaa cccgccagca
     8941 tggttaatac cgccgctgca ctcagaattc ctgccatatt cttgttcata ttccgtcctc
     9001 ttgtttatca gcgtgttaga taagcctgga atacattggg cgctttttca agcccgcgaa
     9061 cgaaacggct ccgctttcag aggattcctg tatgacgttt taaccaccat tcagcccgct
     9121 gtcgcttgtc gtttcagtgg caacgggtta gctttaagga agttttgtct tttctgtctg
     9181 gaggggttca atgacattgc aacaacaaat aataaaggcg ctgggcgcaa aaccgcagat
     9241 taatgctgaa gaggaaattc gtcgtagtat cgattttctg aaaagctacc tgcaaactta
     9301 tccgttcatt aaatcactgg tgctcgggat cagcggcggt caggactcca cgcttgccgg
     9361 aaagctgtgc cagatggcga ttaatgagct gcgcctggaa accggcaacg aatcactgca
     9421 atttattgcc gtacgcctgc cctatggtgt tcaggccgac gaacaagatt gccaggatgc
     9481 cattgccttt attcaaccgg atcgcgtatt aaccgttaat atcaagggcg cggtattggc
     9541 cagcgaacag gcattgcggg aagcaggcat tgaactgagc gattttgtcc gtggcaatga
     9601 aaaagcgcgt gagcggatga aagcacaata tagcattgcg ggtatgacca gcggtgtcgt
     9661 ggtgggcacc gatcatgcag cagaagccat taccggattc ttcactaaat atggtgacgg
     9721 cggtacagac attaatccgc tgtatcgtct caacaaacgt cagggtaaac agttactggc
     9781 ggcattaggt tgcccggaac acctttataa gaaagcgcca acggccgatc tggaagatga
     9841 tcgcccttct ctgccagatg aagtggcact cggcgtgacc tatgacaata tcgacgacta
     9901 tctggaaggg aaaaacgtac ctgaacaggt cgccagaaca atagagaact ggtatctgaa
     9961 aaccgaacat aaacgccgtc cgccaattac cgttttcgat gatttctgga aaaagtaata
    10021 atttgcacat aatggtttgt gcgaaaaaga gtaatttgtt cacgccggat gcggcgtgaa
    10081 cgccttattc gacctacaaa actatgcaaa ttcaatatat tgcaggagtc gaataggcct
    10141 gacaggcgta gcacgtcaga cggtgtaacc tttgtcatcg acccgcctct tttttaatcg
    10201 ctccccgcct gttacactgg atagataacc agcattcgga gtcaacagtg gtacggcgtt
    10261 taacttctcc gcggctcgaa tttgaagctg cggcaattta tgaatatccc gaacatttac
    10321 gttcattcct taatgactta cccacccgac caggggtgta tctgtttcat ggcgaaagtg
    10381 acaccatgcc gctctatatc ggcaaaagcg ttaatatccg cagtcgggtg ctttctcatt
    10441 tacgcacccc ggatgaagcc gccatgctac ggcaatcccg gcgaattagc tggatctgta
    10501 ccgcaggcga aatcggcgct ctgctccttg aagcgcgatt aatcaaagaa caacagccgc
    10561 tgtttaataa acggttgcgc cgcaatcgcc agctctgtgc cctgcaatta aatgaaaagc
    10621 gcgtcgatgt ggtgtatgcc aaagaggtgg atttttcacg agcccccaac ctgtttggcc
    10681 tgtttgccaa taggcgcgca gctttgcaag cattgcagac catcgctgat gaacaaaaac
    10741 tttgttatgg cctgctggga ctggaaccgt taagtcgcgg tcgtgcatgt tttcgttcag
    10801 cgctaaaacg ttgcgccgga gcatgctgcg gtaaagagag ccatgaggaa catgcgctac
    10861 gcttgcgcca gtctctggag cgtttgcggg tggtgtgttg gccttggcaa ggtgcggtgg
    10921 cactgaaaga acagcacccg gaaatgactc aatatcatat tattcaaaac tggctgtggc
    10981 tgggggcggt taattcgctg gaagaagcga caacgttaat tcggacaccc gccgggtttg
    11041 atcacgacgg ttataaaatt ctttgtaagc cgctgctttc cggtaactat gaaattactg
    11101 aacttgatcc ggcgaatgac cagcgagcca gttgatttca ctgaacaaca gtttaccttg
    11161 cggttgtaaa agccgcaggg tgtgcctgcc atcgaaccag cacgcgccct ggtcggtagt
    11221 gagcaattta tcgcccagtt gccacgcccc gttaatcaca aacaccaccc cgccgcgtga
    11281 gccaaaggta gtaaacgtac gttcggcaat tctgactttc gccttacaga catccaggcg
    11341 tgtcatgatg ttgaaatcca tcgacatctg cccttccgtc agtttcgctt ttaccacctg
    11401 gtccgctgca aaggcaaaag gctgtaacgg ttttaaggta tggttaaagc ggtctgcgct
    11461 ttcaaggagc atctcgccgc cttccagcaa cgtcactatc ctttccatgc cgggaaacaa
    11521 agaaaactcc ccattcgccg cgatggatgc aatgctggca cgccagtaaa aatcacgttt
    11581 tgccggtggg aaagtgcaaa tttcgcgcgt ttcaccggca gcatttcgcc acagattcac
    11641 cgacatttta cgcatatcaa agtattccat actcgctccc ggcccgttct tttcattgtt
    11701 atcgttcagg taaccgtgga tggttatcgg caactcgggt gatttacgtt agtggtgatc
    11761 aggctttttt tacaactgtt tgagaagaga aaagaaaacc gccgatcctg tccaccgcat
    11821 tactgcaagg tagtggacaa gaccggcggt cttaagtttt ttggctgaaa gattattcag
    11881 cagttgcagg cattttacct tttg
//
LOCUS       DAEEKQ010000118        11808 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.119, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000118 DAEEKQ010000000
VERSION     DAEEKQ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11808
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.119"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..488
                     /locus_tag="KLK57_003015"
     CDS             <1..488
                     /locus_tag="KLK57_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0893322.1"
                     /translation="NVRRNLTILDMFGPPKTNAGIRTVTLLQPALEALKEQYKLTGHH
                     RKSEITFYHREYGRTEKQKLHFVFMPRVCNEKQKPYYSVSSLGARWNAAVKRAGIRRR
                     NPYHTRHTFACWLLTAGANPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARL
                     S"
     gene            540..1475
                     /gene="ttcA"
                     /locus_tag="KLK57_003016"
     CDS             540..1475
                     /gene="ttcA"
                     /locus_tag="KLK57_003016"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HBE0893323.1"
                     /translation="MQENQQITKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="KLK57_003017"
     CDS             complement(1604..2977)
                     /gene="dbpA"
                     /locus_tag="KLK57_003017"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HBE0893324.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAVISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     SRVRLLK"
     gene            complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="KLK57_003018"
     CDS             complement(3455..4438)
                     /gene="zntB"
                     /locus_tag="KLK57_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HBE0893325.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            4693..5925
                     /gene="dgcM"
                     /locus_tag="KLK57_003019"
     CDS             4693..5925
                     /gene="dgcM"
                     /locus_tag="KLK57_003019"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HBE0893326.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALNCRLVLRKLTEAEPVI
                     IFEGIEASATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFIHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="KLK57_003020"
     CDS             complement(5946..6509)
                     /gene="smrA"
                     /locus_tag="KLK57_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HBE0893327.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(6839..7747)
                     /locus_tag="KLK57_003021"
     CDS             complement(6839..7747)
                     /locus_tag="KLK57_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0893328.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEDIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGQLDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            7923..9233
                     /gene="abgA"
                     /locus_tag="KLK57_003022"
     CDS             7923..9233
                     /gene="abgA"
                     /locus_tag="KLK57_003022"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HBE0893329.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPTSALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAVTASSPSPQWVAWLQNQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            9233..10678
                     /gene="abgB"
                     /locus_tag="KLK57_003023"
     CDS             9233..10678
                     /gene="abgB"
                     /locus_tag="KLK57_003023"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HBE0893330.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSATP
                     GENGHGCGHNLRGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITNSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKAFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            10715..>11808
                     /locus_tag="KLK57_003024"
     CDS             10715..>11808
                     /locus_tag="KLK57_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBE0893331.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVLHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWSLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDATLVIMLPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPQ
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSN"
BASE COUNT         3007 a         2998 c         2926 g         2877 t
ORIGIN      
        1 tgaatgtcag aagaaacctg acgatacttg atatgttcgg tcccccaaaa acaaatgccg
       61 ggatccggac ggtaacatta ctgcagccgg ctcttgaagc actgaaggag caatacaaac
      121 tgaccgggca tcatcgcaaa agcgaaatca ctttttatca tcgggagtac ggcagaactg
      181 aaaagcaaaa actgcatttt gttttcatgc ccagggtgtg taacgaaaaa cagaaacctt
      241 attactcggt aagcagtttg ggtgcaagat ggaatgcagc agtaaaacgt gctggtattc
      301 gccgccgtaa tccgtaccat acgcgacata cttttgcctg ctggctgttg acggcaggag
      361 cgaacccggc atttatagcc agccagatgg ggcatgaaac tgcgcaaatg gtgtatgaaa
      421 tttacggtat gtggattgat gacatgaacg acgaacagat agccatgttg aatgcgcggt
      481 tatcgtagtt gcaaagtttg cccccaattt gccccattta gtaccagaga actgaaataa
      541 tgcaagaaaa tcaacaaatt acaaagaaag aacaatacaa cctgaacaaa ttacaaaaac
      601 gtctgcgtcg taacgtgggc gaagccattg ctgacttcaa tatgattgaa gaaggcgatc
      661 gcatcatggt ttgcctctcc gggggtaaag acagctatac catgctggag attctgcgca
      721 atttgcagca aagcgcgcca atcaattttt cgctggtggc tgttaacctc gatcaaaagc
      781 aaccgggctt cccggaacac gttctgcccg agtatcttga aaagctgggc gttgagtaca
      841 agattgtcga agagaacact tacggtatcg tgaaagagaa gattccagag ggcaaaacca
      901 cttgctcact gtgttctcgc cttcgtcgcg gtatccttta tcgtaccgca acggaactgg
      961 gggcgacgaa gatcgcgctg ggtcaccatc gtgacgatat cctgcagacg ctgttcttaa
     1021 atatgttcta cggcggcaag atgaaaggta tgcctccgaa actgatgagc gatgatggca
     1081 aacatatcgt tattcgtccg ctggcctact gccgcgagaa agatattcag cgatttgccg
     1141 atgcaaaagc gttcccgatt attccgtgca acctgtgcgg ttcacagcct aacctgcaac
     1201 gtcaggtgat tgctgacatg ttgcgtgact gggataaacg ttatccaggg cgtatcgaga
     1261 cgatgttcag cgcgatgcag aatgtggtgc cgtcgcatct gtgcgatacc aacctgttcg
     1321 atttcaaagg catcacccac ggttctgaag tggttaacgg gggtgatctg gcgtttgatc
     1381 gcgaagagat cccactacaa ccggcgggct ggcagccaga agaagatgaa aatcagttgg
     1441 atgagttacg gctgaatgtg gttgaagtga aataaccagg atggcgcccg atgcgcaagc
     1501 gtatcgggct actcttatgg aggccggata agacgcggcc agcgtcgcat ccggcaatcc
     1561 cgaataagat gtttactctt gcacccggca attcaacatt tcattatttt aataaccgca
     1621 cccggctcgt ttttccttta atcttcccgc cctgtaactg tttccatgct ttatgagcaa
     1681 cagcctgacg gaccgcgaca tagacatgcg ccggatgcac ggcgattttg ccaatatctg
     1741 cgccatcaag cccgatatct cctgtcagtg cacctaatac atcacccggg cgcattttgg
     1801 cttttttccc accatcgata cacaacgttg ccatttctgc ttccagcggc acaatggaac
     1861 tattagctgg cggcgtttgc cagttaagtt ttatctgcaa catgtcagaa atgatattgg
     1921 cccgctgtgc ttcttccgga gcacagaaac tgatcgccag accgctattt cctgcacgag
     1981 ctgtacggcc gatgcgatgt acatgaactt cagggtccca cgccagctca aagttcacca
     2041 ccagctcaag cgatttaata tccagaccac gcgcagcaac atcagtcgca accagtacac
     2101 gggcgctacc gttagcaaaa cgtaccaggg tctgatcgcg atcgcgttgc tccaaatcgc
     2161 cgtgtaatga caatgcactt tgccctactt cattcagcgc gtcgcagaca gcctggcaat
     2221 cttttttggt attgcaaaac accacgcaag aggatggctg atgcaagctt aataaccgtt
     2281 gcaacagagg aattttgcct ttgctggatg tctcataaaa ttgttgttca atgggtggca
     2341 aagcatctgt tgagtcaatt tcaatcgcca aaggatcgcg ttgcactcgt ccgctgatta
     2401 cggcaatggc ttccggccag gttgccgaaa acagaagcgt ctgtcgagat gcaggcgcaa
     2461 aacggatgac atcatcaatg gcatcgctaa atcccatatc cagcatgcgg tcggcctcat
     2521 ccatcaccaa agtattcagc gcatccagtg ataccgtgcc tttttgcaag tgatccagca
     2581 aacgacccgg cgttgccacg ataatatgcg gcgcatgttg caacgaatca cgctgcatac
     2641 cgaacggttg accaccgcac aacgtcaaaa ttttggtatt tggcagaaaa cgcgccagcc
     2701 gacgcaattc acctgccacc tgatccgcca gttcacgcgt aggacacagc actaaagcct
     2761 gggtttgaaa tagcgacgca tcaatttgct gtaacaagcc gaggccaaaa gccgccgttt
     2821 tgccgctgcc ggttttcgcc tgcacgcgaa catcttttcc ggcaaggatc gccggaagcg
     2881 cggcggcctg caccggcgtc atagttaaat aacccaactc attaaggttc gtgagttggg
     2941 cgggaggcaa aacattcagg gtagaaaaag cggtcacaat ctattctcgt ggtcatcgac
     3001 gcaaagttag caggcgcgta tcctcgcaga tctacgctca cgatgcgaca atttaatcgg
     3061 ttcttcatcg ggtggtgggt caggcatggg ttgcgggcga gggatcggat cgggcactgg
     3121 aacaggatcg ccaggaatcg gttcagggac aggaatttgc aaataaataa gtgtcgtcat
     3181 atttccctcc ggtcattggg tggactctta aagggtagac gctgataaat aacaggcaaa
     3241 aaaaagccga ctcatcaaag tcggcgtcgt acgaatcaat tgtgctatgc agtaattcaa
     3301 aaaaggaagt aagacaatat ggagcgcaac gcccatcgct tgacgttgca ttcacctgca
     3361 agggagatat tgccctgaat gggtagagag tttattgact tcgctcaaac tttgcggcgt
     3421 ttttgtatac agacagccgg aaaaattgct tttgttacaa ccatttacta cggtgcaacc
     3481 ataaagcaac accaccaata agaacaacta acagaataca aaaaattgaa aatccgaatt
     3541 gccacccgcc gccagggatc ccaccaaggt tgacgccaaa taacccggtc agaaaggtac
     3601 tgggtaaaaa gaccattgcc atcaacgaca ttgtataggt acgacgagct aaattttcct
     3661 gcatcacctg agcgatttca tccgccatca cgccagtccg tgctatacag gcgtcgattt
     3721 cgtcaaggcc gcgcccaagg cgatcggcaa tatcctgcat ccgacggcgt tggtcatcgc
     3781 tcatccacgg caaacgttca ctggcaagac gagcataaac atcacgttgc ggtgccatat
     3841 agcgacgcat cacaattaat tgtttgcgca gcagagccag gaatccacgc ggtggaattt
     3901 gctgatcaag gagattatct tcaaggtcga taattttatc gtgcagctgc tcgataaatt
     3961 cgctggaatg atcggtcaac gcatcgcaca catccaccag ccaccctccg caatcggtcg
     4021 gacccgtgcc ctcttccaga tcgctcacca catcgtccag cgccagcact ttgcgttgtc
     4081 gggtcgaaac aattaaccgc ccgtccatat atacacgcat ggcgaccagt tgatcggggc
     4141 gttcatcggt gctgccgttt atacagcgca atgtaatcag cgtgccttca ccgagacggc
     4201 tgactcgggg ccgcgtgctc tcgcccgcca gcgcatcacg tacgttattg ggaagcagcg
     4261 gtgttgtcgc cagccattgg gcgctatcat ggtgtacata attaaggtgg agccagcagg
     4321 gatgcgcttc atcaatcaca tctgtatttt ccagcggttt aacgccgcct ctaccatcca
     4381 gcatccaggc aaatactgca tccgggacat taacgtccga tcccttaatc gcttccacag
     4441 tgcctccatc atcaacgcat tattttgtag tctagccttc tggccctgtt acgcaacatc
     4501 tcatcacccc attaccctga aatgattaat aaaattctgt ctaaattgaa tacaaaaagc
     4561 aaaatgcttt tccgtataca aaccgtgtga agtgttaaat agcgtctatc attatcagaa
     4621 ttatctgatc atatgacgtg gcttttttgc gatcggatag caacaaaaat tgataaaaat
     4681 aacgggatct caatgattac gcacaacttc aataccctgg acttactcac cagtcctgtc
     4741 tggatcgttt cgccctttga ggaacagtta atttatgcca atagcgcggc gcgactgttg
     4801 atgcaagacc tcacgtttag tcagctacga accggtccct attccgtctc ctcacaaaaa
     4861 gaactgccga aatacctctc cgatctgcaa aaccaacacg atattatcga aatcctcact
     4921 gttcagcgta aagaagagga aacagcatta aactgtcggc ttgttttgcg aaagctgaca
     4981 gaagcagaac cggtgattat tttcgaaggt atcgaagcgt cggcaacgct gggtttaaaa
     5041 gccagtcgct cggcaaatta tcagcgcaaa aaacaaggtt tttatgcgcg cttttttctg
     5101 actaactctg caccaatgtt gttgattgac ccctcacgag atggccaaat cgtcgatgct
     5161 aacctcgccg cgctcaattt ctatggttat aaccatgaaa cgatgtgcca gaaacatacc
     5221 tgggaaataa atatgctcgg gcgtcgcgtc atgcctatca tgcatgaaat ttcgcattta
     5281 cccggtggtc ataaacctct gaattttatt cataagctgg cggatggttc gactcgtcat
     5341 gtgcagacct acgccgggcc gattgaaatt tatggcgaca agctcatgtt atgtattgta
     5401 catgatatta ctgagcaaaa acggctggag gagcagctgg aacatgctgc ccaccatgac
     5461 gcgatgaccg gattactgaa tcggcgacag ttttatcaca ttaccgagcc aggccaaatg
     5521 cagcatctcg ccatcgccca ggattacagc ttgttgctca tcgacaccga tcgttttaaa
     5581 cacattaacg atctctatgg gcattctaaa ggtgatgagg tgttatgcgc cctcgcccgc
     5641 accctcgaaa gttgcgctcg caaaggcgat ttggtgtttc gttggggagg cgaagagttt
     5701 gtcttattgc taccaagaac cccactggat accgcgcttt cgctggctga aactatccgc
     5761 gtaagcgtgg caaaagtgag tatttcgggc ttaccacgct ttaccgtcag cattggtgtg
     5821 gcgcatcacg aaggaaatga aagcatcgat gaactgttta aacgcgttga tgatgctttg
     5881 tatcgggcga aaaatgatgg acgcaaccgc gtgctggcgg cataagccgc ggatgcgtct
     5941 cgagatcaac gactgcgctt agcgtggcgc tcccagtttt cttgcttcgc ctgcgccgtt
     6001 ttacgtagtg cgacgtaaca cgccccgctg ccgccatgat gcggtagcgc agtgcaatat
     6061 gcctgaacat catcaaattc ggtcagccag cgcgccacat agctgcggac aatattggca
     6121 tgcgatttat catcccgtcc tttgccatga ataatcagca cgttacgcaa accatcagcc
     6181 atggcttgtt gaatgaaact gaacatcatt ttgcggcact cttccaccgg ctggcgcaaa
     6241 agattcaggc tcgcctgttg cggatattta ccactgcgca gcttatccag caccccatgt
     6301 tgcaaccctt cccgccgaaa ctccagcggc tgacttagtg ggatgatgtc gagaaatccg
     6361 gtggtgagga aattatcaag ctgcagcgtg tcgatacgct gcggcgcacg ttggttacgc
     6421 gttggatgcc agtggacatc ggtagcacgt ttcagcggct ggacatcttc catggcgtca
     6481 agaaacagcg atttgtcgtc aaggttcatg ttacatcctc ccgcaattaa gagcgcgata
     6541 tgataaccag accgggtcgg tccaacaacg tattacccaa aattccagta ataagttcca
     6601 aatattgccg atattttaag caaaatgctt atgcatgatt attcattcac gatattaata
     6661 atgtaactta tattttcgtg aaatctgtca ctgaagaaaa ttggcaacta aaggttaaaa
     6721 ccgttataac acagtcaccg gcgcagagga gacaatgccg gatttaagac gcggatgcac
     6781 tgctgtgtgt actgtagagt ctggcggatg tcgacagact ctattttttt atgcagtttt
     6841 aactttgcag atagccgcat tctcgccgga attgcgtgat taatgacgcg gtcagtggtg
     6901 tctggcgact atcacgccgc tgaatcaaat aataggccgc tttcggtaaa atttcgctaa
     6961 ccggcaacat caccagcccc tgtccgtgca agggatcgca gcccatttct tcaggcagta
     7021 tgctgagaaa atcgcttttt gccaccagac tgatacaggc tgagaacgtc tcgcagacta
     7081 caccgacctg tggcgtttgc gcctgatcgt caagcaattc actcaactgt ttgtagtaac
     7141 tgccgtgtgg cgtcggcatt gtccagctgt aatccagtaa ctgtttgatc gaacgggcac
     7201 caatggcggg gtgtcccggg cggcaaaaga tcgcgaattg cttttccagt aatttctcaa
     7261 aagtaaattc gtggtcgtac ggtccctgat aataggtatt gatggtgaaa tccaattgtc
     7321 cctgacgcaa ttcattaatc atcgacacca gttgcccttc cataatgcgt acttttacct
     7381 gcggatgctg ctgatgaaaa cgagatatga cagctggcat cagactgcgg gaaatactgg
     7441 cccccatgcc gatattaatc tgccctgcca gttgcccttg tcgttggcga atatcctctt
     7501 gggctgcgcg cagctcttca agaataagac tggcgtgctg ataaaaactt tcaccggcat
     7561 cagttaacgt cacgccttta ctacggcgaa aaaagagttg cgccgctaat ccttcttcca
     7621 gctcctgaat agatttactc agtgccggtt gcgacatatt caacattcgg ctcgctccgc
     7681 gaatgctgcc ctgacgagcc acttcaacaa aagcccgaat ttgatgaatt tttacctgaa
     7741 aagccatgac gccaccgata accgttattt atcagaccaa agaaactggc atctacttta
     7801 atgcagatga ttgtgtcagg gtaatttatg aacggttaaa actgtgaaaa atcagttagt
     7861 gataagtaaa aactatcgct acgtgaaccg ggtcacactt tttactgatg acgggaaagg
     7921 ttatggagtc tttgaatcaa tttgttaatt cgcttgcccc aaaattatcg cactggcgac
     7981 gtgattttca tcactatgca gagtctggct gggtggaatt ccgcactgcc acccttgttg
     8041 cggaagaatt gcagcagctc ggctattcac tggcgctggg ccgcgaagta gttaatgaaa
     8101 gtagccggat gggactacct gatgaactca ctctacaacg cgaattcgag cgcgctcgtc
     8161 aacagggtgc gctagcacaa tggattgcgg tttttgaagg tggtttcact ggtatcgtcg
     8221 ccaccctgga taccggtcgc cccggtccgg tgatggcttt ccgtgtcgat atggacgcgc
     8281 tggatctcag tgaagagcag gatgtcagcc atcgccccta ccgcgacggt tttgcgtcat
     8341 gtaacgccgg aatgatgcat gcctgtggtc atgatggaca taccgccatt gggcttgggc
     8401 tggcgcatac ccttaaacaa ttcgagtccg gactacatgg cgtcatcaaa ctgatttttc
     8461 agcctgcaga ggaaggtacg cgtggcgcgc gggcgatggt cgatgcaggt gtcgtagatg
     8521 atgttgatta ttttactgcc gtgcacattg gcactggcgt acctgcgggc accgtggtgt
     8581 gcggcagtga taattttatg gcaaccacca aatttgacgc gcacttcacc ggtaccgccg
     8641 ctcacgcagg cgcaaaacca gaagacggtc acaatgcctt gttggcggca gcacaagcca
     8701 ctcttgcact gcatgcaatc gccccgcaca gcgaaggagc ttccagagta aacgtgggcg
     8761 ttatgcaggc aggaagcggt cgtaacgttg ttcctacctc ggcgttgctg aaagtggaaa
     8821 cacgcggggc cagcgacgtc attaatcaat atgtttttga ccgtgcacaa caagcgattc
     8881 agggcgcagc aaccatgtat ggtgtcggcg ttgaaactcg tctgatgggt gcagttaccg
     8941 ccagttctcc ttcgccgcaa tgggtcgcat ggttgcaaaa tcaggcggct caggtcgcgg
     9001 gggtcaatca ggccattgaa cgtgttgaag cgcctgcggg ttccgaagat gccacattaa
     9061 tgatggcccg cgtgcagcaa catcaagggc aagcctccta cgtggtgttt ggcacacagc
     9121 tggcggcagg tcatcacaac gaaaaattcg attttgacga gcaggttctc gctattgccg
     9181 tcgaaacgct ggcgcgcacc gcgctcaatt ttccctggac gcgaggtatc tgatgcagga
     9241 aatctatcgt tttatcgacg atgcgattga agccgatcgc caacgttata ccgatattgc
     9301 cgatcaaatc tgggatcatc cagaaacacg ttttgaagag ttctggtcag cggagcatct
     9361 ggcttcggct ctggaatctg caggcttcac cgttacccgc aacgtaggca atatcccaaa
     9421 tgcctttatt gcttcgtttg gtcaaggcaa accggttatc gccctgctgg gagaatatga
     9481 cgccctggca ggtttaagtc agcaagctgg ttgcgcgcaa cctacatccg cgacgcccgg
     9541 tgaaaatggt cacggttgcg gacacaattt gcggggaacc gccgcctttg ccgctgcaat
     9601 agccgtcaag aaatggctgg aacaatatgg gcaaggcggc acagtgcgct tttatggttg
     9661 tcctggcgaa gaaggcggct cgggtaaaac gttcatggtc cgcgaggggg tatttgatga
     9721 tgtggatgcg gcactcacct ggcacccgga agcctttgcc ggtatgttca atacccgcac
     9781 gctggcaaac attcaggcat catggcgctt taaagggatc gcagcacatg ccgcgaattc
     9841 ccctcatttg ggacgcagcg cccttgatgc cgtaacgttg atgaccactg gcaccaactt
     9901 cctcaacgaa catattattg aaaaagcgcg cgtacactat gccatcacaa atagcggcgg
     9961 gatctcgccc aacgtggtcc aggcgcaggc agaagtgctt tatcttatcc gcgcccccga
    10021 aatgaccgac gtgcagcata tttatgatcg ggtcgccaaa atcgccgaag gtgcggcatt
    10081 gatgaccgaa accacggttg aatgccgctt cgacaaagcc tgttccagtt atctcccgaa
    10141 tcgcacctta gaaaatgcca tgtaccatgc cctatcccat tttggtaccc cggaatggaa
    10201 ctccgaagaa ctggcttttg cgaaacaaat tcaggctacg ctcaccccca acgatcggca
    10261 aaacagtctg aataatatcg ccgcaaccgg tggcgaaaac ggcaaggctt ttgcactacg
    10321 tcatcgtgaa acggtactgg cgaatgaggt cgctccatat gccgccaccg ataacgtgct
    10381 tgcggcatcg actgatgtcg gcgacgttag ttggaaactg cctgttgccc agtgtttcag
    10441 cccctgtttt gccgtcggta caccgctaca tacgtggcaa ctggttagcc aggggcgaac
    10501 atctattgct cataaaggaa tgctgctggc ggcgaaaact atggcagcaa ccacagtcaa
    10561 tctcttcatt gattcagggc tattgcaaga atgccaacaa gagcatcagc aagtaacgga
    10621 cacgcaaccg tatcactgcc ctatcccgaa aaacgtgaca ccgtcacctt taaaataaca
    10681 acaacaacgc aaacacaaca accgaggaat gcccatgagt atgtcatcca taccgtcgtc
    10741 ctcccaatcc gggaagctct atggctgggt cgaaagaatt ggtaacaagg ttctccatcc
    10801 ttttttgctc tttatctatt tgattatcgt actcatggtg acgacggcaa ttttgtcggc
    10861 ctttggcgtc agtgcgaaaa acccgaccga tggaacgccg gtggtggtga aaaacctgct
    10921 cagtgtggaa ggattacact ggtctttacc caatgttatt aaaaacttta gcggttttgc
    10981 tccacttggt gcgatcctgg cgctggtttt aggtgccggt ctggcggagc gcgtcggctt
    11041 actgccagcg ctaatggtta aaatggcatc gcatgttaat gcccgctacg ccagttatat
    11101 ggtgctgttt attgcttttt tcagccacat ttcttccgat gcgacgttag tgatcatgct
    11161 accgatgggt gcgctgattt ttctggcggt gggcaggcat ccagttgcag gtttactggc
    11221 tgccattgca ggcgtaggtt gcggctttac ggctaattta ctgattgtca caaccgacgt
    11281 gttgctgtcg gggatcagca cggaagcggc ggctgcgttc aatccgcaaa tgcacgtcag
    11341 tgtaattgat aactggtatt ttatggccag ctccgtagtc gtactgacga ttgttggcgg
    11401 cctgataacc gacaaaatca tcgagccacg gttaggtcaa tggcagggaa acagcgatga
    11461 gaaactgcag acattgaccg aaagtcagcg ttttggttta cgcatagcag gtgtcgtatc
    11521 gctacttttt attgctgcga ttgcgctgat ggtgatcccg gaaaacggga tattgcgcga
    11581 tccgattaat cacaccgtga tgccatcacc ctttattaaa ggtatcgtgc cactgatcat
    11641 tctttttttc tttgttgtct cgctggctta tggcatcgct acccgcacaa ttcgacgtca
    11701 ggcggattta ccgcagttaa tgattgaacc gatgaaagag atggcaggat ttatcgtgat
    11761 ggtttttccc ctcgcccagt ttgtcgccat gtttaactgg agcaacat
//
LOCUS       DAEEKQ010000119        11780 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.120, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000119 DAEEKQ010000000
VERSION     DAEEKQ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11780)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11780)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11780
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.120"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..611)
                     /locus_tag="KLK57_003025"
     CDS             complement(<1..611)
                     /locus_tag="KLK57_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HBE0893332.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPIGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRR"
     gene            complement(734..2380)
                     /gene="fumB"
                     /locus_tag="KLK57_003026"
     CDS             complement(734..2380)
                     /gene="fumB"
                     /locus_tag="KLK57_003026"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HBE0893333.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="KLK57_003027"
     CDS             complement(2458..3798)
                     /gene="dcuB"
                     /locus_tag="KLK57_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HBE0893334.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="KLK57_003028"
     CDS             complement(4369..5088)
                     /gene="dcuR"
                     /locus_tag="KLK57_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HBE0893335.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTDWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="KLK57_003029"
     CDS             complement(5085..6716)
                     /gene="dcuS"
                     /locus_tag="KLK57_003029"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HBE0893336.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLANSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            6897..7127
                     /locus_tag="KLK57_003030"
     CDS             6897..7127
                     /locus_tag="KLK57_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HBE0893337.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVATVVKVIDTCPSGALKYRHK"
     gene            7139..7411
                     /locus_tag="KLK57_003031"
     CDS             7139..7411
                     /locus_tag="KLK57_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE0893338.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFAKHEFDKTREYDDIRS"
     gene            7638..7934
                     /gene="ghoS"
                     /locus_tag="KLK57_003032"
     CDS             7638..7934
                     /gene="ghoS"
                     /locus_tag="KLK57_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBE0893339.1"
                     /translation="MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEY
                     GIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDIDFDLNIMTVDDYFRQFYK"
     gene            7962..8135
                     /gene="ghoT"
                     /locus_tag="KLK57_003033"
     CDS             7962..8135
                     /gene="ghoT"
                     /locus_tag="KLK57_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HBE0893340.1"
                     /translation="MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITW
                     PMSLPVALLFSLF"
     gene            complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="KLK57_003034"
     CDS             complement(8254..9771)
                     /gene="lysS"
                     /locus_tag="KLK57_003034"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HBE0893341.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKNNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIITEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="KLK57_003035"
     CDS             complement(10008..11465)
                     /gene="dtpC"
                     /locus_tag="KLK57_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HBE0893342.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMVIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(11524..>11780)
                     /locus_tag="KLK57_003036"
     CDS             complement(11524..>11780)
                     /locus_tag="KLK57_003036"
                     /EC_number="4.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="constitutive; catalyzes the formation of cadaverine
                     from lysine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HBE0893343.1"
                     /translation="TEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEF
                     LQMLCEIGTHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
BASE COUNT         3045 a         2856 c         2892 g         2987 t
ORIGIN      
        1 aagcgccgta gctggcggca atgaaagcga gcgaggaata tcaccgtcgt taatgcgcca
       61 agcagcgcgc agaacatatc ggattgcgtg tcccactggt cgccctgggt gccgagaaaa
      121 tcatccgctc cctggcccat tgccagcgcc gcccaccact cgattaattc atacatcgcg
      181 cttatcgcca gcgccacgca gcagactagg aacgccacca ttttacgtcc gcgcacgtac
      241 atcccgcgca cgagaatttc tcgtgccacc agtgcaggca ccagcccctg gaaaaaatgc
      301 cccagcttgt catacggatt acggctcaac cccagccatt cctgcacctc aaaaccgatg
      361 gggactttcg cgtaggtgta ttgcccgccg accatcagga tgatggcgtg aaagaaaatg
      421 agcgtataga gcagcggcgt taacggataa cgtttagcgg tggcaagcag tagcggcacg
      481 acaataatca ccggtgtcac ttccatcagc caggtgagtt tgtcatgggc cgaaatgccg
      541 gtataaatca ggattaacgt tagcgccagt gcgccggtgt taagaattaa cggtttgagt
      601 gtgcgggtca tggtgagttc gcaagtcagg gaaaatactg actattcacc agcaagcatg
      661 aaattacaat cgcaaaagag gtggcccagg gggatcacct ggcagcatgc tgccaggcgc
      721 tgggccgaag aggttactta gtgcagttcg cgcactgttt gttgacgatt tgctggaaga
      781 agtcgttacc tttgtcatcg accaggataa acgccgggaa atcttctact tcgattttcc
      841 agatagcttc catacccagc tccggataag cgacgcattc cagatgcttg atgctctgct
      901 gcgccagtac cgccgccgga ccgccgatgc taccgaggta gaagccgccg tgtttatgac
      961 acgcgtcggt aacctgctgg ctgcggttgc ctttcgccag catgatcatg ctgccgccgt
     1021 gggattgcag cagatccaca taggagtcca tacgacctgc ggtggttggg ccaagtgaac
     1081 ctgatggata accggcagga gttttcgccg gacccgcgta gtagatcggg tgatctttga
     1141 tgtactgcgg aagttcttta ccggcgtcaa tcagctcttt cagcttggcg tgtgcaatat
     1201 ctcgacccac aataatggtg ccagtgagcg acaaacgagt ggaaaccgga tattgcgaaa
     1261 gctgggcgag gatctctttc atcgggcggt taaggtcgac cttcaccgct tcgccttcac
     1321 cggcctgacg cagttcttgt ggaatgtact gacctggatt gtgttccagt ttttcgatcc
     1381 agataccttc gcggttgatt ttcgctttaa tgttacggtc agcggagcag gagacgccca
     1441 tgcctaccgg gcaggatgcg ccgtgacgtg gcagacggat aacgcgaatg tcgtgcgcga
     1501 agtatttacc gccaaactgc gcgccaagac cgagtttctg ggcctcttcc agcagttcct
     1561 gttccagctg gacatcgcgg aacgcctgac catgttcgtt cccttccgtc ggcagttcat
     1621 cgtaatagtg agcgcttgct aatttgacgg ttttcaggtt ggtttccgca gacgtaccgc
     1681 caatcacaaa cgcgatatgg tacggcgggc aggctgcagt accgagggta cgcattttct
     1741 cgacgaggaa gtttttcagt ttgccgggag tcagcagggc tttggtttcc tggtagagat
     1801 acgttttgtt ggcagagccg ccgcctttcg caacgcaaag gaatttgtac tcatcgccat
     1861 ctaccgcgta caggtcgatt tgcgcaggca ggttagtgcc ggtgttgacc tctttgtaca
     1921 tgtccagcgc cgcgttttgc gagtagcgca ggttatcttc gatataggtg ttatagacgc
     1981 ctttcgacag cgcttcttca tcaccgccac cggtccacac gcgctggcct tttttaccga
     2041 cgatgatcgc ggtgccggta tcctggcagg tcggcagcac gcctttggcg gcgatttcgg
     2101 agtttcttaa gaattgcagt gccacgtact tgtcgttttc gctggcttct ggatcgtgaa
     2161 gaatagccgc aacctgtttc tggtgtgccg gacggagcat gaaagaagcg tcgtgaaagg
     2221 cttgctgcgc cagcagggtc agggcttctg gttccacttt caggatggtt tcgccgtcga
     2281 agtcggcaac gctaacgtaa tcggaagtga gtagatagta ttcggtattg tctttcccca
     2341 tcgggaaagg tgcctggtag ataaagggtt tgtttgacat agcttccagc ctgtaactct
     2401 gtatttgtta ttcgaaaatg gcgtgccgcg tagtcacggg cggcacgcaa agtgcattta
     2461 taagaacccg tacatcgcgg cgaagatcca gccgaagacg cacgatacgc tcacaccaat
     2521 caaccccggc agaataaagc tgtggttgat gacgaagcga ccgatgtggg tggtgccgga
     2581 acggtcaaac tgaatcgctg ccagatcgct cggataagtc ggcaggatgt aataaccgta
     2641 gcaagccggt gctgaagcca cgatgtatgc cggatcaacg ccgatcgcca gcgcgaccgg
     2701 aacaatcgcc gccagcgccg cagcctgaga gtttacaaac ttggaaacca gcagcagaac
     2761 aatggcatag gcccacggat actctttcac catttcaccc agtacgccct gaatttcaga
     2821 catatgcgca ccgaacatgg tttctgccat ccatgcgata ccgtacaccg ccacgatggc
     2881 gatcataccg gaacggaaga cttcgttttt tgagatagac gcgggattgg ttttggtcag
     2941 gataataatc agcgccccgg tcagcagcat aaacatctga ataaccagta ccatcgacag
     3001 cggtttgccg ccgaaggatg gacgcaggtc cgaatcagca ccaagaaggg cgactacagc
     3061 gattgccccg aggaaaatcc acattgccag ccagttgctt ttcggcagtt ttttgtccag
     3121 cagcgtcgcg gtatcaccgt aaacatactc acggttttcc ggtacggaga tgaatttctg
     3181 gaactcttcg tctttatcca gatctttacc acggaaccag ctgaagatac cgatcgccag
     3241 gataccgatt aacgtcgatg gaatggtgat tgccagcagg tcgaggaact caagatggcg
     3301 accatcaaag gtgacattac ccagcattgc aaccagagac acgaccgcaa ccgacaccgg
     3361 actggcgatg atccccatct gtgcaccgat agaacttgcc gccatcggac gttccggacg
     3421 gatgttgttc ttaatggcga cgtcgtagat gatcggcaga atggtgtaaa ccacatgacc
     3481 cgtaccgcaa agaatggtca gcgtacaggt cacaaacggc gcgacaattg agacatattt
     3541 cgggttgcgg cgcagcagct tctcggcaat ttgcagcatg acatcaagac cgcccgaagc
     3601 ttgcaaggtc gccgatgccg ccaccaccgc aatgataacc agcatgacat caaccggtgg
     3661 tttacctggc tgaaggtgga agacgaagac cagaatgacc agaccgatac cgcctaataa
     3721 acccagcgcg ataccaccct ttctggcacc ataaaacaga catatcagta ttatgataag
     3781 ttggatagta aataacatgt gtgaaccctc gcgataatcc tatttaaatt tttgctgaat
     3841 agatcacagt cacgttctgt tttgtatgaa ctgttttcag agcctgacta aatatccgtt
     3901 tggtcgttac cggctttagc aaatacctca cagtgaatat tggctgatta aagcagcggc
     3961 aaatttatgc actgtgataa gcggcttttt cagagagaaa gactctctgg cgatgggttt
     4021 atagtttgta ctgcgcagat gttaatgttt tgtttgctgt tatcttattg atatttatcg
     4081 gtctattttt tgttctcagg gcagatgtca ttaggtttat agattaatct gatctaccca
     4141 tttgtgggta aaaatacaca taatgcgggt gacataatag ttaattaact tttgttagcg
     4201 ttttgaaatt aaaaacaccg ttcacctgaa gagatattaa tttttagcga tgatgcaggg
     4261 ataattatat ttgatctggc acaagtttta ctgatgaagg atgtaacttg tgccaggggt
     4321 attttgcatt acggtaataa ttattactta cagataacca gcgatccgtt attggcaata
     4381 ttgtttcagt agtgagtagt gttctgcctg aatacggtaa cggtaaactg gacgccccgt
     4441 gacgccataa tggatactgg tgaacaagat gtggcagttg accagccaga tgaggtattt
     4501 acggcaggaa acacgcgaaa tgttaacctc gttggctagc tcgtcggttg aaaattcata
     4561 gtcctgatgc gcgtcaatcc actggcacag tgtgcgtaac gtctgcggcg ttaagccttt
     4621 tggcaagcga cgaggatcct gttcgttgga gctgctgccg tggattagct gatcaagctc
     4681 ggcctggtca taatactgat gtttttccag cgccattttc ttttgccgcc agtcggtgag
     4741 cgcctcttca aagcgggaag cctggaaggg tttgatcagg taatccacga caccgtaatg
     4801 cagcgaatct ttaatggttg ccgcatcggc tgcggaggag atgacaatca catcactttt
     4861 gcaacgcgcg ttatgcagga caggcagtaa atcgagcccg ttctcttttt gcatatagat
     4921 atcgagcaat atcaggtcga taggcgtatc gctattgaag ataatctctt tggctttctc
     4981 cagcgtcgag gctgttccac agcattgaaa gcctgggatt tgtgctacgt atcggcgatt
     5041 cagctccgcg accattgcgt cgtcatcgat aattaataca ttgatcatct gttcgacctc
     5101 tccccgtccc agggtatctg gacaaaaaat tgtgtgaaaa tcccgggttc cgattccacg
     5161 gcgatgctgc cgccgagatt ttctacctgt tgtttgacaa gtgctaaacc gacgcctcgc
     5221 tcgcttcctt ttgtcgagac acctttgtca aaaatgtgat cgattttgtc gggtgcgatc
     5281 cccggtccat catcgttaac ttcgcagtgc agccagccgt gacggtagtg caatgttacg
     5341 ctaatttcgc caccgggttc cggccctaat gcttccagcg cgttttctat cagatttccc
     5401 aatgtggtaa tcagcgtcgc gacctggtcc tcactgccgc tgtctggcag ctggctttca
     5461 ctgtttaaaa tcagcgtatg gcctaaatcg gtcgcgcgat taatcttgct gattaaaaaa
     5521 ccagcgataa ccggagattt gatcttaccc agcagagagc caatctcttc ctgatagtta
     5581 ttggctgttt ttagaatgta atcttccaac tgcttataac tcttcagatg caataatccg
     5641 agaatcacgt gcaatttatt cataaattcg tgggatcgtt cacgaagtgc gtcggcatag
     5701 ttgaccagac cgtcgagtcg ctgcatcagt ttacgtactt cggttttgtc cctgaaggtt
     5761 gaaatggcac cgatgataac gccattactg cgcaccggaa cggtgttgat cagtaatagc
     5821 cggtctttaa tcgtaatctc ttcgtcgcgg cgcggcgtac cgtcgcgtaa cacttccgag
     5881 acatctacca cctgtgacca tgagtggctg agcgtcgaca gtttctcatc gtcctgcgac
     5941 ttacggtaat tcagcaattc ttgtgcggca tcgttgatca gcgtgacctc gccgcgatcg
     6001 tccacggcaa cgacgccttc tttgatagac tgcaacatgg cctggcgttg ctcaaacagc
     6061 gtggagattt cgtagggttc caggccgaaa aggatttttt tcagtacctt aaccagaatg
     6121 caggtgccaa tcagtccgac cagcatgcca aataataccg accagataat gctccagcga
     6181 ctgtcattga tttgttgggt cacgcggctt aactcaaggc cgatcgccac cacgccaatt
     6241 tgtttatgat tttcatcgta gatgggggta aatacgcgta aagcctgcgc cagaaaaccg
     6301 cgattgatag cgacattttc tttgccattc agcgctttaa ggatgtcatc acctttaaat
     6361 ggctgaccaa tacgctgggc ttcaggatgc gagtagcgaa gactttgcat atcggtaacg
     6421 acaataaaca gcagatcgtt gcgtttgcgc acggcttccg cgatggcctg gatgccgctc
     6481 tcctgcggtt ttttctgcaa gccctgacgg atttccggcg agttggcgag ggtacgcgcc
     6541 actgccagtg ccttgttggc tagcccgtct cgcgtcatat cactgatttg cgagaagtaa
     6601 atcagatgca ccaccaatag caccgagaac agtaccgcac tgaccattaa gatcactgtg
     6661 gtactcaatt tcatcggacg tttgcgtaac atgcggtagg gcaatgaatg tctcatcagc
     6721 ttccttgtgt gacaaatttc ttaaacatta tctctgatga ggtgggtaat tcaaagggag
     6781 taagaatgat tggctatata ggggaagaga ttctggcaac ggaaactgcc agtgctgtat
     6841 gaagattcca gggctatgct tatagcgata atcatactga tgagagaggg aaagtcatgg
     6901 atcaggcgct actggacgag ggttatcgct gttataccgg cgaaaagatc gatgtctatt
     6961 tcaacactgc gatatgtcag cattctggca attgcgtacg tggcaacggc aagttattta
     7021 atctcaaacg aaagccgtgg atcatgccgg atgaagtcga cgtcgccact gtggttaaag
     7081 tgattgatac gtgcccgagc ggcgcgctga aataccgtca taaataagcg agggtaaaat
     7141 ggaaatacgc gaaggccaca ataaatttta cattaatgac gaacaaggca agcaaatcgc
     7201 tgaaattgtc tttgtgccga ctggagagaa tttagcgatt atcgaacata ccgatgtcga
     7261 tgaaagcctg aaagggcagg ggattggtaa acagctggtt gcaaaagtcg tggaaaaaat
     7321 gcgtcgggaa aaacgaaaaa ttatcccact atgcccattt gcgaaacacg aatttgataa
     7381 aacgcgggag tatgatgata ttcgcagttg atgggagagt acagagtcac gatatttttc
     7441 atgctctccg cggagtgatg caggagagca tctgaagggt agggggatgc acaaagaatg
     7501 ggcagagagc gcgttttttt gtcccaagtc atccccttta ctgagcaaaa aaaagaatat
     7561 ctcctatatg agaatcatca atcggggtta tcaagttttg cgtccccaga gcattcaata
     7621 ttgataggag tcatattatg gaaggtaaaa acaagttcaa tacttatgtt gtttcttttg
     7681 attatccatc atcttattcc tcagtgttct taaggttaag atcattgatg tatgatatga
     7741 atttctcttc tatcgtggct gatgaatatg ggataccacg acaattgaat gaaaattcct
     7801 tcgcaataac gacatcgtta gccgcaagtg aaatcgaaga tttaatcagg cttaaatgct
     7861 tagacttacc ggatattgat tttgacctca acattatgac agttgatgac tatttccgtc
     7921 agttttacaa gtagccagga aggataagaa gaacataaaa aatggcacta ttctctaaaa
     7981 tattaatttt ttatgtgatt ggtgtgaaca tatcctttgt cattatctgg tttatctcac
     8041 atgagaaaac acatattcgt ttacttagtg cattcctggt cggaataacc tggccaatga
     8101 gtctgcctgt ggcattactt ttttctctct tttaggagct gtagttgagt ggacccgctg
     8161 gttatcgtga aacgaagcgt ctctcctcaa aactggattt gcggaacgac gaacgaagca
     8221 gtcgcctgac tgcttcgttc attaaagtga aatttatttc tgtgggcgca tcgccgggaa
     8281 gagaataacg tcgcgaatag tatgactgtt tgtaaacagc ataatcattc ggtcgatacc
     8341 aatacccaga ccagcggttg gcggcagacc atattccagc gcagttacgt aatcttcgtc
     8401 atagaacatg gcttcgtcgt cacctgcagc tttagcgtta acctgttcct ggaaacgttc
     8461 agcctgatct tctgcgtcgt ttaattcgct aaaaccatta ccgatttcac gaccaccgat
     8521 gaagaattca aaacggtcgg tgatttccgg gttaacatca ttacggcgcg ccagcggtga
     8581 cacttctgcc ggatattccg taataaaggt tggctgaatc agatgtgctt ctgccacttc
     8641 atcaaagatc tccgtgataa tacgtcccag cccccagctt ttctctaccg taattccgat
     8701 agattcagct aatgctttag cagcatcaaa attatccagg tcggccatat cggtttctgg
     8761 acgatatttt ttgattgctt cgcgcatggt gagtttttca aacggtttgc cgaaatcaaa
     8821 cacatgctcg ccataagtga ctttagtggt acccagaacc tcttgtgcca gggtgcggaa
     8881 cagtgactct gtcagttcaa tcaaatcgtg gtaatccgca tacgccatgt agagttccat
     8941 cattgtgaac tcaggattat ggcgaacaga aataccttca ttacggaagt tacggttgat
     9001 ttcgaatacc cgttcaaaac cgcctacaac cagacgtttc agatacagct ccggcgcgat
     9061 acgcagatac atatctaaat ccagagcatt atgatgggta ataaacgggc gagcagatgc
     9121 cccacctgga attacctgca tcatcggggt ttctacttcc ataaagccgc gcgcgaccat
     9181 gaactgacgg atagcggcca gaatttttga acggacaaca aacgtttgac gggatttatc
     9241 gttagcgatg aggtccagat aacgttgacg ataacggact tcctgatcct gcagaccatg
     9301 gaatttatct ggtaaaggac gtagtgcttt agtcagcagg cgcaactcag tacagtgaat
     9361 ggaaagctca cccgtttgcg tcttaaacag cgtaccgcgg gcaccgataa tgtcacccag
     9421 atcccatttt ttaaactgat cgttataaac accttctggc aggctatctc ttgcaacgta
     9481 cagttgaata cggccaccga catcctgcaa cgttacaaag gaggctttcc ccatgatacg
     9541 acgggtcatc attcggccag caaccgagac ttcaatgttt aaggattcca gttcctggtt
     9601 attcttcgca tcaaactctt cgtgcaactg gtcagaggta tggtcgcggc gaaaatcatt
     9661 gggaaacgcc acaccttgct gacgtagtgc cgccagtttt tcgcggcgat ttctcagttc
     9721 atcgttaaaa tcaatagcct cattggctcc ccgtgtttct tgttcagaca ttttggttcc
     9781 tctaaatcca gctttcaatt tagcgtagat aaagagacag atcggtacac tgtattatct
     9841 gcctcgacta ttaataactc agtttatctt atcgaaacta acgcttacct caagttgata
     9901 tcactaataa ataaccatca aaatggttta aatgacttat taatctcgat ttactagtgt
     9961 gattaactga aagttaattt attcataacc aattgttttt acgacagtta atcgttgctc
    10021 tcctgtatca tattcgtcgg cgtgctaaac agaaaacggg tagcaaaggc caatacgacg
    10081 atgatcgcga cacaagccaa cgtccactct cccatctgag aaaagaatcg ctggtaagct
    10141 gcaattacca taccgctaat ttgcgactct gtcgtctgct gtgccacgac gcctgccagc
    10201 cagttggcga ccgcgcctgt tgccagcata taaataccgg ttaatacgcc agacattttc
    10261 agacgcgtga tttgcgcaat cgccaccggg tcaataaaga gttctgcaaa gcccattagc
    10321 gccaacccgg atatcatcac gcccattgac gcttgaccgt cagccgctgc atgtcgggca
    10381 tcaaatgcca acaacataaa gccacaagcc atcagcagta agccaaaggc aaacttcagc
    10441 cagacgcgca atgttgagtt gccgcggctt tctggcgacg ccagccaggc cagtacaacc
    10501 ccagcgagca tcactgcaat tgcattcacc gactggaata gtgctgtagg tacttcaata
    10561 ttgaatgcct gacgattcac aaagcgatcg ataaacaagc tgatggtact gccgccctgt
    10621 tgtgccagta cccagaacaa cgtcccgaca aacatcaaca atacaatttg ccaaagagca
    10681 cggcggtgtt cggggaattt gatcatcatg cgggcaatga tttgtgcggc aatgaggcaa
    10741 acgatcgcca gcaaatatcc ggaccagtcg ttctccagca gcagggtaaa aaatactggg
    10801 gctaaacaga gcatcaccac taaccagctc catactggta aggcaaattt gacgctagtg
    10861 agcgcttttt tatccatact acgcgtggac tggaaatgac gatgaccgct taagaaaatc
    10921 aacaaaccga taaacatgcc gccacccgca agggcaaagc caacatgcca tccataccac
    10981 tgagcagcca ggccgcaggc gattggggcg gcgatagaac cgatattgcc cgcagcatac
    11041 agcagcgaaa aaccgccatc acgtcgatga tcgttctcgt cgtagagctc gccaagcaaa
    11101 cagctgatgt ttgatttgaa taaaccgtag ccacaaataa tgattgccag cgccagatac
    11161 aggctaaagg ttgaatttgt atcaatgccc agcaccacat ggccaagggt cattaacagc
    11221 gcgccggcaa tcactgcagt gcggttgccg agcaggcggt cggcaagcca gccgccgaga
    11281 ataggggtaa cgtaaaccag agaagcatat gcgctgaaca ggctgatggc atggttatca
    11341 tcaaaaccaa gctgatgggt gagatagaga atgagtaagg cacgcatgcc gtaaaaactg
    11401 aagtactccc agatttggat cgccacgata tagtatatcg cgcgcggctg tgagggtgtt
    11461 ttcatgtgtt ctccttatga gcaaaaaagg gaagtggcaa gccacttccc ttgtacgagc
    11521 taattatttt ttgctttctt ctttcaatac cttaacggta tagcggccat cagcctgacg
    11581 gtatgcaccg tgaatatcgg tttcaaatcc cggatagtga gtgccgattt cacacagcat
    11641 ctgcaggaac tccagaaccg gacggctttc ttcggtgatc atttcacccg gcattaccag
    11701 aggaactccc ggcggatacg gaaggatcat attggcgtta atacgaccta ccatttcgtc
    11761 gaggtaaact tcttcggtca
//
LOCUS       DAEEKQ010000120        11622 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.121, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000120 DAEEKQ010000000
VERSION     DAEEKQ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.121"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..357
                     /locus_tag="KLK57_003037"
     CDS             <1..357
                     /locus_tag="KLK57_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HBE0893344.1"
                     /translation="INDYLYGCLLSLYSICARDIYNMSAGNNVKVAADAFLEIIFSLK
                     NKYCIKLLSAQERAFIYEFARMIFAYINDKSIEILLLSCFAASDQKAIQRYIPQSQDG
                     EDFRSHLQYKLPLSAH"
     gene            568..891
                     /locus_tag="KLK57_003038"
     CDS             568..891
                     /locus_tag="KLK57_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HBE0893345.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            888..1718
                     /gene="amiD"
                     /locus_tag="KLK57_003039"
     CDS             888..1718
                     /gene="amiD"
                     /locus_tag="KLK57_003039"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HBE0893346.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPTQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(1715..2728)
                     /locus_tag="KLK57_003040"
     CDS             complement(1715..2728)
                     /locus_tag="KLK57_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HBE0893347.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENTVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPIITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(2827..4257)
                     /locus_tag="KLK57_003041"
     CDS             complement(2827..4257)
                     /locus_tag="KLK57_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HBE0893348.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWQVVNQIGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVD
                     SWKVIVVEPEKQLTLLFGMKASGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(4268..5269)
                     /gene="ltaE"
                     /locus_tag="KLK57_003042"
     CDS             complement(4268..5269)
                     /gene="ltaE"
                     /locus_tag="KLK57_003042"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HBE0893349.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRKRN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(5306..7024)
                     /gene="poxB"
                     /locus_tag="KLK57_003043"
     CDS             complement(5306..7024)
                     /gene="poxB"
                     /locus_tag="KLK57_003043"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HBE0893350.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKADRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(7157..8125)
                     /gene="hcr"
                     /locus_tag="KLK57_003044"
     CDS             complement(7157..8125)
                     /gene="hcr"
                     /locus_tag="KLK57_003044"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HBE0893351.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(8137..9789)
                     /gene="hcp"
                     /locus_tag="KLK57_003045"
                     /gene_synonym="priS"
     CDS             complement(8137..9789)
                     /gene="hcp"
                     /locus_tag="KLK57_003045"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HBE0893352.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKACEYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(9933..10832)
                     /gene="lysO"
                     /locus_tag="KLK57_003046"
     CDS             complement(9933..10832)
                     /gene="lysO"
                     /locus_tag="KLK57_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HBE0893353.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(11327..>11622)
                     /locus_tag="KLK57_003047"
     CDS             complement(11327..>11622)
                     /locus_tag="KLK57_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HBE0893354.1"
                     /translation="LVVELVLSAGFLLVIHGATDKFAPAGFAPIAIGLALTLIHLISI
                     PVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT         2829 a         3073 c         3080 g         2640 t
ORIGIN      
        1 ataaatgatt atttgtatgg ctgtttacta tctctgtact ctatctgtgc aagggatata
       61 tataatatga gcgccgggaa taacgtgaag gttgctgcgg atgctttcct tgaaattatt
      121 ttctctctta agaataaata ttgcattaaa ctattaagcg ctcaggaacg ggcgtttatt
      181 tacgaatttg caagaatgat cttcgcttat attaatgaca aaagcattga gatcttatta
      241 ctttcttgtt ttgcggcgtc tgaccaaaaa gcaatccaga ggtatatacc tcagtcacaa
      301 gatggggaag actttaggag tcatttacaa tataagttgc ctctttccgc tcattaatgt
      361 tgttggtgca acataataag acaggtaagc ggtaccctat aatgaaacca atatcgggtt
      421 atgcaacgga taatcgtaaa gacttaagtg ggtgtttttg acacaatgag atgatttcct
      481 aaactgatca aaaccttctt cccaacatgg catatcagtt cgataatgct ttagagatcg
      541 aaggcttaac aaacaaggag atcgatcatg caattttcta caaccccaac tctggaaggc
      601 cagaccatcg ttgaatattg cggtgtggtg accggcgaag cgattttagg tgccaatatt
      661 ttccgtgatt tctttgccgg tatccgcgat atcgttggcg gacgttccgg tgcgtatgaa
      721 aaagaactgc gtaaagcacg ggagatcgcc tttgaggaat taggctccca ggcgcgggcg
      781 ctgggggccg atgccgtcgt cggtattgat atcgactacg aaacggtcgg gcaaaacggc
      841 agtatgctga tggttagcgt cagcggtacg gcggtgaaaa cgcgtcgatg agaagagtct
      901 tctggctggt cgctgccgct ctgttattgg cagggtgtgc aggcgaaaaa ggcattgtcg
      961 agaaagaggg atatcagctt gatacccgac gccaggcgca ggcggcgtat ccgcgcatta
     1021 aagtgctggt gatccactac accgcagatg attttgatag ctcgctggcg acactgaccg
     1081 ataagcaggt cagctcgcat tatctggtcc ctgcggtacc accgcgatac aacggtaaac
     1141 cgcgcatctg gcaactggtg ccggaacaag aactggcctg gcatgcgggg attagcgcct
     1201 ggcgcggggc aacgcgcctt aacgacacct ctattggcat tgagctggaa aaccgtggct
     1261 ggcaaaaatc ggccggagtg aaatattttg ccccgtttga accgacacag attcaggcgc
     1321 ttattccact ggcgaaagat attattgccc gttatcacat caagccggaa aacgtagtgg
     1381 cacatgcgga tatcgcaccg cagcgcaaag acgatccggg gccattattt ccctggcagc
     1441 aactggcgca gcaggggatt ggtgcctggc cggatgcgca gcgggttaac ttttaccttg
     1501 ccgggcgcgc gccgcacaca ccggtagata ccgcgtcatt gctggagctt ttggcgcgct
     1561 acggttatga cgttaaacct gatatgacac cgcgcgagca gcggcgcgtg attatggcat
     1621 tccagatgca tttccgcccg acgttatata acggcgaagc ggatgcagaa actcaggcga
     1681 ttgccgaagc attgctggag aaatacgggc aggattagcg cggcagtttt ccgtggtcgc
     1741 gtagccaggc ggcagttttc tcgatacctt catctagggt gatgatcggc tgataaccta
     1801 actcttcctg cgcacgcgta atatccagcg taaagtcaaa attcaacttg gagacgccgt
     1861 agtgggtcag cggcggctct tttgctgact tgcggcctaa acgctccatg ctgcgggcga
     1921 tcatatccag catcgggtag gggacggaac gaatacgaca gtcaatattc aactcgtcga
     1981 tcagcttctg cacgatgctg cgcagtgtgc gatgctcgcc gttggtgata ttgtacacac
     2041 gcccggaagg tagcttatcg caggcttcct ggcttgccag ccacattgcg tgcacggtat
     2101 tttcatagta ggtcatatcg accagcgcac tgccgccatg cggtaacaga atactgccgt
     2161 agtggtgcat catatgcgcc agacggggaa taaagacttt atcgtgcggt ccgaacagac
     2221 tttgtgggcg cagaatagta aagcgcgttt gtggattcgc ctgcgaaagc atattgatca
     2281 cttcttcgct ggctgctttg ctgcgggcaa actcgttggc gaagcggtga gggcgaaaat
     2341 cttctttaat atcgcgatgg tggtgataat cgaagtacag ggagggggaa gagatatgaa
     2401 taaagttacg cacaccccag gcgacagccc attcacccag gcggcgagtg gcgcgaacgt
     2461 tagccagatc gaaagcctgt tgtgtccccc agggtgaggt aaagctggag cagtgccaca
     2521 gcgtatcaat gcccgcgagc atcactttag cttgtgatga aaccagctcg gtcagatccg
     2581 ccggaacaaa ctctgcgccc attttttcca gcaatttgcc cattgcctcg ttgcgaccgg
     2641 tcgctcgcac gctgatgcct ttctggcata aaaactctac cgcgtttcga cctaagccgc
     2701 tggtggcgcc ggtaaccagt accttcatat caatccactg ttgttgagaa aatgacgtgc
     2761 gcattcttcc gtgatttccc ccatgatgca atgggaaaca tgaaagaata acgcaggttt
     2821 tgtcgattaa tctgtgcttt gttctgccag tctggcgatt tgttttgcca ttccgcgaaa
     2881 aataaacaga tgcgcgggga tcatcaataa ccagtaaaac agccccggca taccgtgcgg
     2941 atgccagaaa gcgcggacat cgatagtacg atagtcgcct ttatcttcca ggctaaaaca
     3001 cagtcgtccc agccccgacg ctttcatgcc aaataacaac gtaagttgtt tttccggttc
     3061 aacgacaatc actttccagc tatccaccgc atcgccagtc tgtaaatatt cgcgctccgg
     3121 gcggcctttc gccagcttat gaccgatcgc gcggtccatc aacgcccgtg tctgccacaa
     3181 aatattgcca aagaaataac gctctttacc gccgatttgg ttcactacct gccataaagc
     3241 agcaaggctg gcggacgttt taacggtaaa ccccgcctgt ttggcaaaat aaccgtactc
     3301 cggtcgccag cgggcaaagg cctgagcgtc gtagccccag tcgctggagt tgaccagttt
     3361 ttcctcctct ttcaacgtgc tacgtaccgc gtcatcgaaa gcgatcagcc gttgtgggat
     3421 gagtgcacgt agcgcggtat catccgccag cagatcgtgt ttcagcccct gaatcaacgc
     3481 cctggcggtg gtgggcggta cggaagtaat cacattgaga aaccacaccg aaatccagcg
     3541 ggtggggagg gggatgggga tcaaccagcg gcgcttaccg ctcaccgcca taaaatgttc
     3601 aaactgttgc tgataactga gcacctctgg tccggcggct tcgaagatgc ggtgttcgct
     3661 ggccggatga tctaacaacg ccaccagata gtgcagcaag ttttccagcg cgatgggcgt
     3721 ggtgcgtgaa cgtacccagc gtggcggcgt taacactggc aggttgtaga ccatatcgcg
     3781 catgacttcg aacgccgctg aacctgcgcc aacgataatt ccggcccgaa gttcggtcac
     3841 aggtacattc gcttcacgaa gaatgtccgc cgtagcctga cgagcacgca gatgatccga
     3901 ctgctcatgt ggcggggcct gcaacgaact gagaaagatt aattgcttaa ctggtacttc
     3961 acgtagcgca tcgcggacgt tgagagccac ctggcgctcc tgagcgataa aatcgccgcc
     4021 ttcgcccatg ctgtgcacca gaaaatagac ggtatcgata tcctgcaaca gggccggaag
     4081 gttatccggc cagctgagat cgactttatg gcaactgacg tttgccagtt gcagctttgc
     4141 aagcctgtcg acatgacgtg ccgccgccag gatctgatgc ccttgctggc tgagtgtgcg
     4201 caccagatgc tgaccaatgt agccactggc accgagaact aaaatgcgtt gcggcacgtc
     4261 tctctcctta acgcgccagg aatgcgcgcc agtgggcggc gacttccgcc agttgttcgc
     4321 gcgagacgtc aagatgcgtc accaggcgga caatcggcga ggcgttaatc agcacgtttc
     4381 tcgctttcat gtattcgcct aacgcggcag cattttcttc cccgacgcga acaaacagca
     4441 tattagtgtc ctgacgcatc acatccgcgc ctgcttcacg cagttgctcc gccatccagg
     4501 cggcgttgtc gtgatcttcc tgcaaccgtg cgacgttatt tttcagggca tacatcccgg
     4561 cggcagccag aatcccggac tgacgcatcc cgccaccggc cattttccgc cagcggatgg
     4621 cacgtttaat gtaatcacga ttaccgacga gtaatgaacc gactggcgtc ccaagacctt
     4681 tcgacaggca aatggtgaac gaatcacaat attgcgtgat ctctttcagt tcgcagccgt
     4741 aagccaccac ggcattaaag atgcgcgcac cgtcaacatg cagcgccaga ttgcgcttgc
     4801 gggtaaattc ccatgcttct ttcaggtatt cacgcggcag cactttgccg ttgtgggtgt
     4861 tttctagact gagtaatttg gtgcgggcga aatggatatc gtcgggtttg attttcatcg
     4921 ccactttatc cagcggtagc gtgccgtcgg cagccgcgtc gatgggttgc ggttgaatac
     4981 tgcccagcac cgctgcgcca ccggcttcaa acagataatt atgcgcggcc tgaccgacaa
     5041 tatactcttc gccgcgttcg cagtgactga gcagagcgac caggttagcc tgggtgccgg
     5101 tcggcagaaa aatggcggct tctttaccgg aaagctctgc ggcgtagtcc tgcagagcat
     5161 taacggtagg gtcgtctccg taaacgtcgt ccccaaccgg ggcggccatc attgcttcga
     5221 gcatggcgcg gctcggtcgg gtaacggtat cactgcgtaa atcaatcatg gcatgtcctt
     5281 attatgacgg gaaatgccac cctttttacc ttagccagtt cgttttcgcc agttcgatca
     5341 cttcatcgcc gcgtccgctg atgattgcgc gcaacatata caagctaaat cctttggcct
     5401 gttcgagttt gatctgcggt ggaatggcta actcttcttt ggcgaccacc acatccacca
     5461 gcaccggacc gtcgatggag aaggcgcgtt gcagggcttc atcaacttca gacgcttttt
     5521 ctacacggat acccgtaatg ccgcacgctt cggcaatgcg ggcaaagttt gtgtcgtgta
     5581 gttcggtgcc gtcagtcaaa tagccaccag ctttcatctc catcgccaca aagcccagca
     5641 cgctgttgtt aaagacgaca attttcactg gcagtttcat ctgcactact gagaggaaat
     5701 cgcccatcaa catgctaaaa ccgccatcgc cgcacatggc gaccacctga cgttctggct
     5761 ctgtcgcctg cgcacccagc gcctgcggca tggcgttagc catcgaaccg tggttaaacg
     5821 aacctaacag gcgacgcttg ccgttcattt ttagataacg tgccgcccac accgttggcg
     5881 taccaacgtc acaggtgaaa atagcgtcat cggcggcaaa atgactaatt tgctgcgcca
     5941 gatattgcgg gtgaatggct ttctcgctcg gtttagctaa atcgtccagc cctttgcggg
     6001 cgtcgcggta atcttccagc gctttatcca gaaacttgcg atcggctttt tcttccacca
     6061 atggaagcaa tgcacgcaga gtcgacttga tatcgccgac cagtgccata tccaccttgc
     6121 tgtgagcgcc gatgctggct gggttgatat caatctgaat gattttggca tcggtcgggt
     6181 agaaggcgcg gtagggaaat tgcgtgccga gtagcactaa cgtgtcggcg ttcatcatgg
     6241 tatggaaacc tgacgagaag ccgattaacc cggtcattcc aacatcatac ggattatcgt
     6301 attcgacatg ttctttaccg cgcagggcat gaacaatagg cgctttaatt ttcccggcaa
     6361 actcaactaa ctctttatgc gcccccgcgc agccgctgcc acacatcagg gcgatattgc
     6421 tggaataacg cagcagttgc gccagtttgc gtaactcttc ttcttccggc gtcacgactg
     6481 gttgtggcgc atgataccag tgcgtggttg ccccttctgg cgcaggtttt aacgccacgt
     6541 cgcctggtaa cacgacaacc gaaacgccac ggttaagcac cgctttgcgc atggcaatcg
     6601 ccagtacttg tgggatctgc tccgggctgg aaaccagctc gcaatagtga ctacattcgc
     6661 ggaatagctc ttgtgggtgg gtttcctgga aatagccgct gccaatttcg ctggagggaa
     6721 tatgagcggc aatcgccagt accggaacgt gattgcggtg gcaatcgaac aggccgttga
     6781 ttaagtgcag gttgccgggg ccgcacgatc cggcgcagac cgccagttct ccgctaagtt
     6841 gtgcttcagc gccagcggca aaggccgcca cttcttcgtg gcgggtggac atccactcga
     6901 tggtgcccat gcgattaaga ctgtcactaa gaccgttcag agagtcgcct gtgactcccc
     6961 agatgcgttt cacccctgcc gattcgagtg ttttggcgat ataagctgca accgtttgtt
     7021 tcatggttct ccatctcctg aatgtgataa cggtaacaag tttagttcat ctgacggagg
     7081 gggaagggat gggagagaaa ggaggcacta acggttaaat agcccgatga aaggaatatc
     7141 atcgggcata aggcgattat gcgagaacca aatccccctg cggatggcag gagcaggcca
     7201 gtacgtaacc ttcagcgatt tcggcgtcgg tcagcgtcat tgtactgctc accgtatatt
     7261 caccggaaac cacttttgtc ttacagcagc cgcaaacacc cgcacggcag gcagcgacaa
     7321 ccggaacgtt attgctttcc agcgcctcca gtagcgtggt gccaaccggg gcgtaaaatt
     7381 ctcgtgccgg ttgcagtttg gtgaatttca gaccgctggt cgccgcttct gctactggag
     7441 tgaagaattt ctctttaaag aaacgcgtca cgccgagcgc tttcacttcc tgctctaccc
     7501 aatccatata cggagccggg ccgcaggtca tcacggtacg tgaagctaag tcaggtacac
     7561 ctgccagcag ttcgcgagtg agacgaccag cgataaagcc ttcggtaacg ttattttctg
     7621 ccaccagcgt taccggatag ttacgccact catcggcgaa aataacatcc tgcggcgtac
     7681 gcacgttgta gatcacccgc acatcggcct gtggacggtt cttcgcaagc cagcgacgca
     7741 tcgacataat cggcgtgacg ccgcagcctg ccgccagcaa caggaattta tcttctgctt
     7801 tatcgtcgca ggtaaattcc cccatcgcgt ccgaaagcca gagataatca ccgcgtttta
     7861 catcgcgcgt cagccactgg gagccgacac cgtcatcaat ccgccgcacg gtcagggtga
     7921 tatattcact cacgcctggc gtggaggaaa tggtgtaagc acgcagcgtt tccgctgagt
     7981 ttcgcacgct gaccagtgca tattgcccgg cgcgatatgg gtagtaatcg tggcaaatca
     8041 gggaaatcgt ccacacatcc ggcgtttctt gcgtaatgtg atgaacctgc atccgccacg
     8101 ggcattgatt cgttggcatc gtcatcgaca aactccttac gcgctcaaca gttgcttcat
     8161 gtcttcttca acagtggtga tagaacgcag gccgaatttc tcgttcagca ccgccagcag
     8221 gtctggtgtc aggaaaccag gtgcagtcgg gccggtgacg atatttttca cgcccagaga
     8281 aagcagcgtc agcagaatga cgatcgcttt ctgttcaaac caggagagca ccagcgacag
     8341 cggcagatcg ttcacaccac agcccagttt ctctgccaga gtgacagcca gaataatcgc
     8401 tgagtaagca tcgttacatt gacctgcatc taccagacgc ggcagacctt cgatatcgcc
     8461 aaactcaagt ttgttaaagc gatatttacc acaggcgagg gtcaggatca ggcagtcatc
     8521 cggcacgctg gtggcgaaat cggtgaagta gtggcgctcg ccgcgtgcgc cgtcacagcc
     8581 accaagcagg aagatatgac gcagtttttc acggctcacc agatcaatca gcgtatcagc
     8641 agcgccaagc agcgtctggc gaccaaaacc cacggtgata aggtgcggaa tttcgctgta
     8701 cgggaagcct gccatctgtt gcgcctgggt gataaccgca gagaaatcat caccgtccag
     8761 atgacgcacg ccaggccagc caacaatgct gcgggtccag atacgatcgt cataagcgcc
     8821 tacggttggg tcgatgatgc agttcgaggt catcacgatg gggccaggga aacgagcgaa
     8881 ctccacttgc tgattctgcc agccgctgcc gtagttaccg accagatgct tgaatttacg
     8941 cagctccggg tagccatgcg caggcagcat ttcgccgtgg gtgtagacat taacgcccgt
     9001 gccttcggtc tgttccagca ggttgtagag atctttgaga tcgtgaccgg aaatcagaat
     9061 gcatttaccc gccgtcgctt tgacgttgac ctgggttggc gtcgggtgac cgtatttacc
     9121 ggtttcgcct gcatccagaa tgctcatcac tttgaagttc atctggccga tttccattga
     9181 acactcaaga agcgcgttca tatcggcagg ccaggtcccc agccacgcca tgattttatg
     9241 gtactgggca taaatatcgt tgtcgtattg accgagaaca tgcgcgtgtt ccatataggc
     9301 cgccgcacct ttcaggccat acaggcacag cagacgcagg ccgagaatgt tttcgccaat
     9361 cgccgcttta tctttgttag gggtaaattc tgctgcctga cgttgcagct cgccgagatc
     9421 atcgctcacc agttgcaggt cagccatcgg gttatcgacg cgcgcgttgg catctacagc
     9481 caggcattgt gctttcagcg cctcgcgcaa ggcaatcgct tcacgagcgt agccgacaat
     9541 acgcggagaa tcgaagttaa cgttggtcag ggttgagaaa aaggcacgtg gcgcgaagct
     9601 gtcaacatcg tggttgatga tgccgtattc acacgctttt accgcccagg cagaaagccc
     9661 ttgcagcgcc gcgatgagta aatcctgaag gtcagaagtt tccgccgttt taccacacat
     9721 cccctgcgcg tatgagcagc cgtttcctgc cggagtacgg atagtttgtt cacattgcac
     9781 acaaaacatg atcacacctt ttaaagttat atttaatata catgtttaag gttaagacgc
     9841 ttaacgcggg gataaaaggg atttttcatg caactttaag ggagattgat ttagcgcaat
     9901 tttggcggca gggatctacc gccagagagg tattacgcag agaaaaaggc gatgaggatc
     9961 ggcactaaca ggctaagaat aaaaccgtga acaattgccg ccgggaccat atccagcccg
    10021 ccagtacgtt gaagaacggg cagggtgaaa tccattgatg tggcaccgca taagcccagt
    10081 gcagtagagc ggctgcggcg aatcagccca gggatcaaca taatagcaat cagttcacgg
    10141 gccagatcat taaaaaacgc cgcgctcccg attaccggac caaaagattc ggtcaataaa
    10201 ataccggaaa gagaatacca gccgaaaccg gaggccattg ccagcgcggt attgatgggg
    10261 agatcaagaa taaaggcgtt aattaaacca ccaattaatg aactgacaac caccaccacg
    10321 gcgacaatca ttccccggcg attaaggaca atctgcttta aggtcatgcc attattgcgc
    10381 aactgaatac caacgaggaa aagtagcaaa attaacgtgt attcactggc ttcggtcgcg
    10441 tgttgtaaga aagccagtcc actcagacca atggcaaaac caatcactac tacgccgcac
    10501 agttttagcg actccagcgc catcgcaata cgcgacggga gtttttcttg ctgatggtgg
    10561 ttgcgccacg gcaggcctcg ctccagccac atcagggcgg caatattaca cagtaaaata
    10621 acggtaatac tgacggcaga ataatgcaga atcgccaaca ggttactggc gaggttatcg
    10681 agaaacgcca gactgatacc cataaaaaag agaataaggt aaaccatcca gcttaatagc
    10741 tgattaataa cttttaacgc agcttgttgg cgaagcggaa tgaggtaacc tacaatcagg
    10801 ggaaccagaa tgattaacag cccagaaaac atgaaaaccc agtccttgca aagatgaagt
    10861 cgaaatgcgc gatgacacac tactgaaagc ggaaggacga gtaaagttgc aattaaaagg
    10921 aaatgttatg cataaggagc agtagagtat tcgttttcat ttaaagatat tcttgcgctt
    10981 taattacaaa ctgcaccgat gttggtggcg tcaaaatcgc cgaggcgttc cctgaaggcc
    11041 ggggcagccc acatggatgt gggctgaggg cgcgttttac agggatgtta cctcgcgccc
    11101 gacccggtag ccgtaaggga taagtcgagg gcaccgcgca gcggcgattt tgttcgccag
    11161 agcccggggg ggcagggggc ggcggcgatt ggccgccccc tgcgcgctcc ttgcgccagt
    11221 ggcaatatgt ggcttagctc atgaaaggag cgcaacaaga tgatgaatca acatataaca
    11281 acatcttaaa aaaaggcctg acattacgcc aggccttctg cgttaattaa tcacgctttt
    11341 ccagcagggt ccggtaaatc agaccaccga taatgccgcc gacaattggc accacccaga
    11401 agaaccacag ttgttctaat gcccagccgc cctggaagat agcaaccgcg gtgctgcgcg
    11461 ccgggttaac agaagtgtta gtcaccggaa tactaattaa gtgaatcagg gttaaggcca
    11521 gaccaatagc gatcggcgca aaacctgccg gcgcgaattt gtcggttgcg ccgtggatca
    11581 ccaacaggaa acctgcactc aataccagtt caactaccag cg
//
LOCUS       DAEEKQ010000121        11567 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.122, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000121 DAEEKQ010000000
VERSION     DAEEKQ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11567
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.122"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            176..847
                     /locus_tag="KLK57_003048"
                     /pseudo
     CDS             176..847
                     /locus_tag="KLK57_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
     gene            857..2053
                     /locus_tag="KLK57_003049"
                     /pseudo
     CDS             857..2053
                     /locus_tag="KLK57_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
     gene            2053..2613
                     /locus_tag="KLK57_003050"
     CDS             2053..2613
                     /locus_tag="KLK57_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBE0893355.1"
                     /translation="MLKRKIIQIADQVIIDETAGEVVIGANTRICHGAVIQGPVVIGA
                     NCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCYIADSVVANQAYLGA
                     QVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRIISPNTQL
                     GPRVIVERNLPTGTYSLRQELIRTGD"
     gene            2615..3388
                     /locus_tag="KLK57_003051"
     CDS             2615..3388
                     /locus_tag="KLK57_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HBE0893356.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3576..3851
                     /gene="frmR"
                     /locus_tag="KLK57_003052"
     CDS             3576..3851
                     /gene="frmR"
                     /locus_tag="KLK57_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HBE0893357.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3886..4995
                     /gene="frmA"
                     /locus_tag="KLK57_003053"
     CDS             3886..4995
                     /gene="frmA"
                     /locus_tag="KLK57_003053"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HBE0893358.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5088..5921
                     /gene="fghA"
                     /locus_tag="KLK57_003054"
     CDS             5088..5921
                     /gene="fghA"
                     /locus_tag="KLK57_003054"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HBE0893359.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6147..6686)
                     /locus_tag="KLK57_003055"
     CDS             complement(6147..6686)
                     /locus_tag="KLK57_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HBE0893360.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYTIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6788..7999)
                     /gene="mhpT"
                     /locus_tag="KLK57_003056"
     CDS             complement(6788..7999)
                     /gene="mhpT"
                     /locus_tag="KLK57_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HBE0893361.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALALLFYSS
                     QIRSTGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8286..9299)
                     /gene="dmpG"
                     /locus_tag="KLK57_003057"
     CDS             complement(8286..9299)
                     /gene="dmpG"
                     /locus_tag="KLK57_003057"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HBE0893362.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVRHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAVLKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9296..10246)
                     /gene="mhpF"
                     /locus_tag="KLK57_003058"
     CDS             complement(9296..10246)
                     /gene="mhpF"
                     /locus_tag="KLK57_003058"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HBE0893363.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10243..11052)
                     /gene="mhpD"
                     /locus_tag="KLK57_003059"
     CDS             complement(10243..11052)
                     /gene="mhpD"
                     /locus_tag="KLK57_003059"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HBE0893364.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDHAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11062..>11567)
                     /gene="mhpC"
                     /locus_tag="KLK57_003060"
     CDS             complement(11062..>11567)
                     /gene="mhpC"
                     /locus_tag="KLK57_003060"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HBE0893365.1"
                     /translation="AFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQLYRQP
                     TIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFPDFGPR
                     LAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADAFNQLV
                     LNFLARP"
BASE COUNT         2785 a         3118 c         2887 g         2777 t
ORIGIN      
        1 acatatgaat tgcataaaaa cattacccag tcgaacctcc gggtctgaaa gcccttgctt
       61 ttcagttatt agcaacattt aacatttata aattaacatc tggctgcgcc agagattacg
      121 tctctgagtg catcgttgtg cttgtagtat aataaataaa atggagtgtc attaagtgga
      181 taaggtttta gattcagccc tcctttcttc ggcaaataaa agaaagggta tccttgctat
      241 tggcgcacat cctgacgata tagaattagg ctgtggcgca tcgcttgctc gtcttgcgca
      301 aaaaggaatt tatatcgcag cagtggtgat gactaccggc aactctggta cagatggaat
      361 aatcgatcgc catgaagaat cgcgcaacgc cctaaagata ttagggtgca accaaactat
      421 tcatcttaat tttgctgaca cccgcgctca tttacagctc aatgatatga tttccgccct
      481 ggaagacatc attaaaaatc aaattccttc tgatgttgaa atcatgcggg tatataccat
      541 gcatgatgcc gaccgccatc aggatcatct cgctgtttat caagcttcaa tggttgcctg
      601 ccgcactatt ccacaaattc tcggctacga aaccccaagt acctggcttt catttatgcc
      661 tcaggttttt gaatccgtta aagaagaata tttcacagtc aaacttgctg cattaaaaaa
      721 acataaaagc taggaacgac gtgattatat gcgtcatgat cgcctgcgtg cagttgcaca
      781 atttcgcggg caacaggtca atagcgatct gggtgaaggc tttgtaattc ataaaatgat
      841 tctttgagca aattgcatga aaacctggat atttatctgt atgtccatag caatgttgct
      901 atggttttta agtacgctaa gacgtaaacc cagtcaaaag aaaggctgta ttgacgccat
      961 tatacctgcg tataacgaag gcccgtgtct ggcgcagtca ctggataatc tactgcgtaa
     1021 cccttatttt tgccgggtaa tttgcgttaa cgacggctcc acggacaata ccgaagcggt
     1081 catggcggaa gtcaaacgca aatggggcga ccgctttgtt gccgtcacgt aaaaaaatac
     1141 cggtaaaggt ggtgcgctga tgaatggcct caattatgcc acctgcgacc aggttttttt
     1201 aagtgatgcc gacacctatg ttccgcccga tcaagacgga atgggctata tgctggcaga
     1261 aattaagcgc ggtgctgatg ccgtaggcgg cattccctct actgcgttga aaggcgcggg
     1321 gctgttaccg cacatccgcg cgaccgtaaa gttgccgatg attgttatga agcgcacgct
     1381 acagcagctc ctgggcggcg caccgtttat tatcagcggt gcctgcggaa tgttccgtac
     1441 tgatgtattg cgtaagttcg gtttctcgga tcgtactaaa gtcgaagacc ttgatctcac
     1501 ctggacatta gtggcaaacg gctaccgtat tcggcaggcg aatcgctgca tcgtataccc
     1561 acaggaatgc aacagtccgc gtgaggagtg gcgtcgctgg cggcgttgga ttgtgggcta
     1621 cgcggtctgt atgcgcctgc ataaaaggct tttatttagc cgcttcggta tcttcagtat
     1681 atttcctatg ctgttggttg tgatttatgg cgttgggatt tatctcacta cctggtttaa
     1741 tgaattcatc accaccgggc cgcatggtgt ggtgttggca atgtttccgc ttatctggat
     1801 cggcgtagtt tgtgttattg gtgcttttag cgcctggttt catcgttgct ggttgttggt
     1861 gcctttagcg ccgctttccg ttgtgtatgt attattagct tatgccatct ggattatcta
     1921 tggacttatt gcctttttta ctggacgcga acctcagcgc gacaaaccca cccgctattc
     1981 cgcactggtg gaagcgtcaa ccgcttattc ccaaccttct gtcacaggaa ctgaaaaact
     2041 atctgaagct taatgttaaa gagaaaaatt attcagattg cagaccaggt aattattgat
     2101 gaaactgcag gtgaagttgt tatcggcgcg aatacccgta tttgtcatgg tgccgttatt
     2161 cagggtccgg tagtgattgg cgcaaactgc ctgataggta attatgcgtt tattcgtcct
     2221 ggcacaataa tcagcaatgg tgtaaaaatt ggttttgcca ccgaaattaa aaatgcggtt
     2281 attgaagcgg aagcaacgat tggtccgcaa tgttatattg ccgactcggt agttgcaaac
     2341 caggcatatt tgggcgcaca agtacgtacc agtaatcatc gtctggatga acagcctgtg
     2401 tctgttcgaa ctccagaggg aattatcgct accggatgcg ataaattagg ttgttatatc
     2461 gggcagcgtt cacgccttgg cgtacaagtt attattttgc ctgggcgaat tatttccccg
     2521 aacacgcaac ttggcccgcg cgtgattgta gaacgtaatt tacctaccgg aacttattca
     2581 ctccgacaag aacttatccg tacaggagat taaaatgata aaacggacgt tattagcggc
     2641 ggccattttt agcgcattgc ccgcttatgc cgggttaact tccattaccg cgggctacga
     2701 ttttaccgat tattctggcg atcatggcaa ccgtaattta gcgtatgctg aactggtggc
     2761 gaaagttgaa aacgcaacgc tgctttttaa tctttcccag gggcgtcgtg attatgaaac
     2821 tgaacatttc aatgccactc gcggtcaggg tgcggtctgg tataaatgga ataactggct
     2881 gacaacccga acgggtattg cctttgcgga taatacgccg gtctttgccc gccaggattt
     2941 tcgtcaggat attaacctgg ccctgttgcc aaaaacgctt ttcacgaccg gttatcgcta
     3001 cactaaatat tacgatgatg tcgaagtcga tgcctggcaa ggcggcgtat cactctatac
     3061 tggcccggtg atcaccagct accgctatac ccattatgac tccagcgatg caggtggtag
     3121 ttatagcaat atgatttccg tgcgtctgaa tgacccgcgc ggcactggtt atacgcaact
     3181 atggctaagc cgcggaacag gcgcttacac ctatgactgg acgccagaaa cacgctacgg
     3241 cagcatgaag agcgtcagtt tgcaacgtat tcaaccgctg actgagcaac ttaatctcgg
     3301 gctgacggca ggtaaagtgt ggtacgacac cccaaccgat gattataacg gtctgcaact
     3361 tgcagcccat ctgacctgga aattctgatt gcttctgccg cccgctatcc ggggcggcct
     3421 tccctgccga ttagccccac tccctttcct cgttgttttc cgaccacatt caccggataa
     3481 attttattct ccagcgttat atactatagg gaggtatgca ttgacatata gcatacctcc
     3541 ctatagtata ttgcgtgcag atgatgaggt gcgaaatgcc cagtactccg gaagagaaga
     3601 aaaaggtcct tactcgagtt cgtcgtattc gggggcagat tgatgctctg gaacggtcgc
     3661 tggagggtga tgccgaatgc cgtgccatac tccaacagat cgctgccgtt cggggcgcgg
     3721 ctaacgggct gatggcagaa gtgcttgaaa gccatatccg ggaaacgttt gaccgaaatg
     3781 actgctacag ccgcgaagtc agccaatccg ttgacgacac tattgaactg gttcgagcct
     3841 atcttaaata gctgaatcta ttaccatatt gaggaagagc gagagatgaa atcacgtgct
     3901 gccgttgcat ttgctcccgg taaaccgctg gaaatcgttg aaattgacgt tgcaccaccg
     3961 aaaaaaggtg aagtgctgat taaagtcacc cataccggcg tttgccatac cgacgcattt
     4021 accctctccg gggatgaccc ggaaggtgta ttcccggtgg ttctcggtca cgaaggggcc
     4081 ggcgttgtgg ttgaagtcgg tgaaggcgta accagcgtca aacctggcga ccatgtgatc
     4141 ccgctttaca ccgcggagtg cggcgagtgt gagttctgtc gttctggcaa aactaacctc
     4201 tgtgttgcgg ttcgcgaaac ccagggtaaa ggcttgatgc cagacggcac cacccgtttt
     4261 tcttacaacg ggcagccgct ttatcactac atggggtgct ctacattcag tgaatacacc
     4321 gtggtcgcgg aagtgtctct ggccaaaatt aatccagaag caaaccatga acacgtctgc
     4381 ctgctgggct gtggcgtgac caccggtatt ggcgcggtgc acaacacagc taaagtccag
     4441 ccaggtgatt ctgttgccgt gtttggtctt ggcgcgattg gtctggcagt ggttcagggc
     4501 gcgcgtcagg cgaaagcggg acggattatc gctatcgata ccaacccgaa gaaattcgat
     4561 ctggcgcgtc gcttcggtgc taccgactgc attaacccga atgactacga caaaccgatt
     4621 aaagatgtcc tgctggatat caacaaatgg ggtatcgacc atacctttga atgcatcggt
     4681 aacgtcaacg tgatgcgtgc ggcgctggaa agtgcgcacc gcggctgggg tcagtcggtg
     4741 atcatcgggg tggcaggttc tggtcaggaa atctccaccc gtccattcca gttggtcact
     4801 ggtcgcgtat ggaaaggttc cgcgtttggc ggcgtgaaag gtcgttccca gttaccgggt
     4861 atggttgaag atgcgatgaa aggtgatatc gatctggaac cgtttgtcac gcataccatg
     4921 agccttgatg aaattaatga cgccttcgac ctgatgcatg aaggcaaatc cattcgaacc
     4981 gtaattcgtt actgatttcc cgcaggtata ccccgtccac ttcagacggg gttcttaata
     5041 ctctccccgg gcagccgtcc gggggattaa ccgtgagata atgactgatg gaactcattg
     5101 aaaaacatgc cagctttggc ggctggcaaa atgtgtatcg gcattattcc caatcactga
     5161 aatgtgaaat gaatgtcggc gtctatctcc caccaaaagc cgcgaatgaa aaattgccgg
     5221 tgctgtactg gctttcaggc ctgacctgca acgagcagaa tttcattact aaatcgggga
     5281 tgcagcgtta cgcggctgag cacaacatta ttgttgttgc gccggacacc agtccgcgag
     5341 gcagtcatgt cgcagatgcc gaccgttacg atctcgggca aggtgccggg ttttacctga
     5401 acgcaacgca agcgccgtgg aatgaacatt acaaaatgta tgactatatc cgcaacgagc
     5461 tgccggattt agtgatgcaa cattttccgg caacgaccag aaagtctatc tctggtcatt
     5521 ctatgggcgg gctgggcgcg ctggtgctgg cgttacgtaa cccagatgaa tatgtcagcg
     5581 tctcggcgtt ttcgcccatt gtctccccat cgcaagtacc gtggggacag caagcctttg
     5641 ccgcatatct tggtgaaaat aaagatgcct ggttggatta cgatccggtg agtcttattt
     5701 cacaaggtca acgcgttgcg gaaatcatgg ttgatcaggg gttgagtgat gatttttacg
     5761 cggaacagct gcggactcca aatcttgaaa agatctgcca ggagatgaat atcaagacgt
     5821 taatccgtta tcacgagggt tacgaccaca gctattattt tgtctccagt tttattggcg
     5881 agcatattgc ctaccacgcc aataaactga atatgcgttg ataatagtgc acgactgccg
     5941 gatgcggcgt gaacgcctta tccggcctac acttcgcccg taaaccgtag gcctgataag
     6001 acgcgcaaag cgtcgcatca ggcatggcgc acgactgccg gatgcggcgt gaacgcctta
     6061 tccggtctac acttcgccca taaaccgtag gcctgataag acgcgcatag cgtcgcatca
     6121 ggcatctgcg cacgacttat ctatgtttac cacatcaaat catcaggcac tttgaagtcg
     6181 gcatacggat cgtcttcatc ctgctcttcg gcactgagcg cgctgtgcaa cacaatactg
     6241 ctggcatcac gctgagcaat tttatcggca acactggcgg gaataatggt gtattcacct
     6301 tcgctattgt tatcgaccaa caagcgggca atcgccagac gaccattaat caactgcgcc
     6361 tgtgtgagct tatcgacaaa aatcttctta atcagattgc cgtcagtgaa gttaaaacca
     6421 atatcgccat tggcaatggt gattcggttc atttcaatga gctgcttcac ctgggcttta
     6481 tattctttcg ccaacgccgc ttgtttttgc tgttcgctaa gctgtttatc acgctcaagc
     6541 tgtgcctttt tattttcttc taccgcagcc cgagcttcac gcgcctgaac gcgcgatttt
     6601 ttcgccgttc tctcgacctt cgccgctttt ttgctggtga ctaatcctgc tttgagcaac
     6661 tgctcttgta aggtaagttt tgccatcttt gtttccaaac ccgctgaata atttttagcg
     6721 agtatacctg taaacgccgt gggtgtattg ggggcaagtc cctttcgccc ctgcttaata
     6781 aggcgcgtca ggcatcggca cacggctgca ttcttgatct ccgactcatc aaaataaaca
     6841 ccgccaaccc agcaacaaga atacccggtg cagaagcggc cattacgccg accgtgccag
     6901 tgcctaatgc cagcattttc ccggccagta acggaccgct catagccccc agacgcccta
     6961 ccgccacggc tgttcccaca cctgttgagc ggatctgcga actgtaaaac aacagtgcca
     7021 gggcatacaa aacgctttgc ccacctgtcg caaacaaccc cgcgacaaat cccgccagca
     7081 acataccgtt aaatgacgac accgttccaa gcgccagcag cgaagctaac atgccgctat
     7141 aaatcagtag cgacatggtt actggacgca gcttatccat caatgcgccc aacattaacg
     7201 tcccgcttgc cgcccccatt tgcagggcaa acatcacccc tgccgcctgc gatggctgga
     7261 atccttgctc caccaaaagt agcggtagcc agttgatcaa catataaacc accagcagag
     7321 tgaagaaata acacaaccac agcagcagcg tcgcggttgc cgtttctggc gcaaataagg
     7381 cacgcagtgg tggcgcagcc tgtttttcgc cagcgaaaac cgctgactcc ggcagccagc
     7441 gcattaatag cggcaccaga atcaacggca ccacaccacc tacccaaaac accgtttgcc
     7501 atgctaagtt tgcccccgcg aaacccagtg tcgccgccag cgccgcgcca atgggaacac
     7561 cgcaatacat caggctcact gccgtcccac gaaaacgtgg acccgcggct tcagatgtca
     7621 gggcgataag attcggcaac gccgccccca gcccgacacc ggtcatcagc cgcgcaaaga
     7681 ccagtgaggg gaaatcccag gcaatcgccg ttgccagtga gaacaaacca aacagcgcaa
     7741 ctgagccaat caaaatacgc ttacgaccat aacggtctgc cagcatcccg ccaaccaacg
     7801 cgccgggtag caaaccgagt attccggcgc taaatatcca gcccatttgc attttatcga
     7861 gtgcgaaagc ctgggcgatg ccacccgccg caatgccagc cgcctgaaga tccagccctt
     7921 ccatcagagc gaccaaaaaa caaagcccga tggtcagcat caggcgggat gaagatgatg
     7981 aaggggtacg agtcgacata agatgaacct cattgcgagt gcaggtgaat cgcgactgat
     8041 acattgccag ccgcgtaatc agaactattt aatgactgcc tacgggcgaa atgtagcgaa
     8101 atgtaggccg gataaggcgt tcacgccgca tccggcagtc gtgcgcagat gcctgatgcg
     8161 acgctaacgc gtcttatcag acctacaaat tctcacgcca tccgtaggcc ggacaaggcg
     8221 ttcacgccgc atccggctgt cgtgctggga tgggcataca aaatgcacgt tatcggcagt
     8281 catcattatt tgttgttgcg cagatccagc gccacgtcga cgatcatatc ctcctggccg
     8341 ccaaccatcc ggcgtttgcc cagctcaacg agaatatcca ccgcacttaa gccataacgc
     8401 gccgccgccg tttcacagtg acgcaggaag ctcgagtaaa caccagcgta tcccagcgcc
     8461 agcgtttcgc gatcgactcg taccggacga tcctgcaacg gacgcaccag gtcgtcggcg
     8521 gcatccatta acgcatagag atcggtccca tgctgccagc ccagtttatc cgcggcggca
     8581 ataaacactt ccagcggtgc gttacctgcg cccgcgccca ttcccgcgag gctggcgtcg
     8641 attcggtcgc agccctcttc caccgccgcg atagagttcg ccacgccaag actcaggtta
     8701 tggtgagcgt gcatgccggt ttgcgtttct ggtttcaaca ctgctttcag ggcgcggaaa
     8761 cggtcacgga tatcgctcat gttcatcgca ccgccagaat ccaccacata aatacaggtc
     8821 gcaccgtagc cttccatcag ctttgcctgc ttggcgagat tctccggcgt ggtcatatgg
     8881 ctcatcatca gaaaaccaac ggtgtccatt ccgagctcgc gggcatactg aatatgctgg
     8941 gcggaaacat cagcttcggt acagtgcgtt gccacacgaa ccacccgcgc gccagcctgc
     9001 caggcatttt tcagatcgtg aatagtgccg attcctggca gcaacaacgt cgcgattttg
     9061 gcgtgcctca ccacatccgc cgccgcttca atccattcaa ggtcgctatg tgcgccgaaa
     9121 ccatagttaa agctggaacc ttgcaaaccg tcgccgtggg ccacttcaat cgaatccacg
     9181 cgggcatcgt ccagtgcttt ggcaatctgg cgaacgtttt ccagcgaata ctgatgacga
     9241 atggcgtgca taccgtcacg caatgtgacg tccgagatat aaagtttttt accgttcatg
     9301 ccgcttctcc tgccttgcgc gccagtgact gggccatttt ttccgctgtc gccagcgcac
     9361 tggaagtcat aatatcgagg ttgcccgcat aggcaggcag ataatgcgct gcgccttcga
     9421 cttccagcca gaccgctgtt ttcagtccgg agaattgccc cacgcccggt aaattgaccg
     9481 gtttatcctg cgggataact tcaaactgca cgcgctgttt caggcgataa cccggtacgt
     9541 aagcctgcac cgcctcagcc atttcattga ttgaggcttc gatatcatct tgtgaagctt
     9601 cgtcgctcaa tacatacacc gtgtcacgca tcatcaacgg tggctctgct gggttaagca
     9661 caataatcgc cttccctttt gccgcgccgc ccaccacttc aatggctcgg gaagtggttt
     9721 ccgtaaattc atcgatattg gcacgcgtgc caggtccggc agatttactg gcgatagaag
     9781 cgataatttc ggcgtaatga acacgcgcca cgcgtgaaac tgccgccacc attggaatgg
     9841 tggcctggcc gccgcaggtg accatgttga cgttcagttg atcgacgttc gcctcgaggt
     9901 taaccaccgg cacgcagtaa gggccgatgg cagcaggcgt caggtcaatt aagcgaatat
     9961 ccggtttcgc ttcgcgtaaa gcggcatcgt ttttcacatg cgcgcccgcg ctggtcgcat
    10021 caaatacaat gtcgatatca gcaaattcag gcatgttcat cagcccgatc accccttcat
    10081 gggtggtggc gacgcccata cgtctggcac gcgccagacc gtcggactga ggatcaatgc
    10141 cgaccatcac cgccatctcc agatgctgac cgtgacgcaa aattttaatc atcagatcgg
    10201 tgccaatgtt gccagaaccg ataatggcga ctttacgctt actcatgaca gacttccttt
    10261 tggggctgcg cttgaaaatg tcgcagcaac tgaacctatg ccttcaatat gggcttcaaa
    10321 acgatcgccc gcattcaccg ccaccatcgg gcctaatgcc ccggtaagaa tgatatctcc
    10381 ggcgcgcagc ggttcaccca gactggccat tttgcgtgcc agccagacgg cggcattaag
    10441 cggatgtccc aggcattcgc tgccgcgccc gctggaaacc tcttcgttat tacgcgtcat
    10501 cttcatggcg cagtttttca ggtctaaccc cgccggacgt tgcgccggac cgccgatgac
    10561 atacaccccg caggaagcgt tatctgccac ggtatcgaca aactgaatcg accagtcgcg
    10621 aatgcggctc cccaccactt ccagcgccgg aagtacccat tcaatggcgt tatacagttc
    10681 gtcgaaggtg atatcagttg cgggtaaatc gcggttcaac accagtgcga tctccgcttc
    10741 aatgcggggt tgcagaacac gggaaaaagg aatgatttcg ttatcgccat aacacatgtc
    10801 ggcaaataac gtcccaaaat ccggttgatc aacgcccagt tgttgttgca ctttcggatg
    10861 cgtcaggccc actttacgcc ctaccacgcg acgcccctgc acaacgtcat attgcacatt
    10921 tatgtgctga atggcgtagg ccgcttcagc gtgatcgata ccaatcagat cgcgcagcgg
    10981 cgcaatcgct tcgccctgct ctgcggcgcg gcgtaaatcc gccgccagtt gctcaagagt
    11041 atgcttcgtc atgaccattc cttaagggcg tgcgaggaaa ttcagcacca attgattgaa
    11101 agcgtcggca tgttcccact gcgcccagtg accacagtcg cggaagatat gcagttcaga
    11161 accggcaatg ccggacagca gacgcagacc cgcatccatc ggcacaaagc ggtcgttgcg
    11221 cccccagaca atcagggttt gcgctttgat ttccgccaga cgtgggccaa aatccgggaa
    11281 ctgtttcgga ttagcttcca ggctcttaac gaagttttcc aggtgatcgc ggcgcgacag
    11341 catattattc aggcgcgctt caaacagggc gtcggtcaaa tcgctggtat cgaaaacgaa
    11401 gatatccatc atcagcttca ggttttcgat agtcggctga cgataaagct gattcagtcg
    11461 cttaatacct tcggttggca tcggcgtaaa caaactcatg ccgcccgtac cgccgcccat
    11521 cagcaccagt ttgccgacgc gctccggcca gttaagggtg aacgcca
//
LOCUS       DAEEKQ010000122        11542 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.123, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000122 DAEEKQ010000000
VERSION     DAEEKQ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.123"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            460..963
                     /locus_tag="KLK57_003061"
     CDS             460..963
                     /locus_tag="KLK57_003061"
                     /inference="COORDINATES: protein motif:HMM:NF026057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0893366.1"
                     /translation="MENWVIQELKSLDIGDSRLDKRVKHILNSLSRSPEESIPVSCRT
                     WSETKAAYRCFSNDKVSANKIMAPHKENITERAQQFKRVLVLQDTTELNYSGQKEKQG
                     VGHKKHKDERNLFLHPQLVISESGVCLGVYDDYQWFRDELKTSKNTRQEICNDLLHKK
                     HVSEKET"
     gene            complement(1336..2529)
                     /locus_tag="KLK57_003062"
     CDS             complement(1336..2529)
                     /locus_tag="KLK57_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0893367.1"
                     /translation="MSPGIEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFVTDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2665..4389
                     /gene="iucA"
                     /locus_tag="KLK57_003063"
     CDS             2665..4389
                     /gene="iucA"
                     /locus_tag="KLK57_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HBE0893368.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            4390..5337
                     /gene="iucB"
                     /locus_tag="KLK57_003064"
     CDS             4390..5337
                     /gene="iucB"
                     /locus_tag="KLK57_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HBE0893369.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            5337..7079
                     /gene="iucC"
                     /locus_tag="KLK57_003065"
     CDS             5337..7079
                     /gene="iucC"
                     /locus_tag="KLK57_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HBE0893370.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFVEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDATSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            7076..8413
                     /gene="iucD"
                     /locus_tag="KLK57_003066"
     CDS             7076..8413
                     /gene="iucD"
                     /locus_tag="KLK57_003066"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HBE0893371.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFARNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            8419..10614
                     /gene="iutA"
                     /locus_tag="KLK57_003067"
     CDS             8419..10614
                     /gene="iutA"
                     /locus_tag="KLK57_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HBE0893372.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IAAGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLSLTKSVNVSDSKLEGVKVDSYELGWRFTGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYCPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(11165..11308)
                     /locus_tag="KLK57_003068"
     CDS             complement(11165..11308)
                     /locus_tag="KLK57_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893373.1"
                     /translation="MEHRSVIHGELAIDHDAILQCAEEVLHHCVIQTVSFVWHILCYS
                     MGL"
BASE COUNT         2926 a         2896 c         2949 g         2771 t
ORIGIN      
        1 ctcaacgaag gggccgacaa acacgttgtc cctgagttca cattcataaa tgttgaccgg
       61 tttgacaata cggacattga tcccataaat cacatcccgg acagcacatt ccagaaattt
      121 catactcaca cgtcctttaa atacagatga catttgccgg aattataccg cccagattca
      181 cttgccatgt ttcagaatag cttataagcc gcacttttac cgatactcag caacaaatca
      241 ggtctgtatc cacttcagcc ggaaaccgga tctgcgcggc ggggaacggg caagaaatat
      301 gataattaag caacacacat aaaatcatat tgcataatcg catctgattc gaacacttat
      361 tcgcatcact atcgatcatc acttcgcatc tcataccctt acccacaagt tgaaataata
      421 gtattatatt gaaaagacag agattatttc agaggtgtta tggaaaactg ggttattcag
      481 gagctaaaat cactggatat aggggactca agacttgata aaagagtaaa acatatcctc
      541 aatagcctca gtcgttcacc agaagaaagt attcctgttt cctgccgtac ctggagtgaa
      601 acaaaggcag cttatcgttg tttttcaaac gataaagtct ctgctaataa aattatggct
      661 cctcacaaag aaaatatcac tgaaagagct cagcagttta aaagagtttt agttttacag
      721 gatacaacag aacttaatta ttctggtcag aaagaaaaac aaggagttgg tcacaaaaaa
      781 cataaagatg aaagaaactt atttcttcat cctcaactcg tcatcagtga gtcaggtgtt
      841 tgcctcggtg tttatgatga ctatcagtgg ttcagggatg aattaaaaac cagtaaaaat
      901 acccgacaag aaatctgcaa tgatttactt cataaaaaac atgtcagtga aaaagaaacc
      961 tagcgttggg ttgaaggata taataaagca acagaactgg ctcgccaatg ccctgatacc
     1021 catgtattgt caatttctga tcgtgaaggc gatttttatg acctgtttga acgcgcagag
     1081 cagactcccg gaataaaagc tgactggctt gtcagaatga aatttaataa cagagcaacg
     1141 ctgaatatta atggtaaacg ggatcatagg ttgttgcacg aaagaatcat ggagattaca
     1201 ccgcagcaac tggttgaatt cactatacct gatgggcggg ggcagcaagc cagaacactg
     1261 cctgacatgc tttcatcaga cgcaggtcct ctctcatatc ctctgattta ctccagtaag
     1321 gtgatgatca tatctttatt tgatagtacg gtaatgccag ataaatacag acacaatggt
     1381 aaataatata gttgcaaata atgctgccat aatcagcgcg attgcgacac ctgttttcga
     1441 taccagcggc accagcaaag cgcacagtcc atagccaagt gtgtgactgg tagcgagcca
     1501 gcccgcgcct gcgccatcag ccagcttatc attcagtaga agttgataag cgggtgttgc
     1561 cagagctgct ccaaatgaca gcactgcaca gcccgggtaa aacaaccata gctgttcgga
     1621 gagcatgata gccagaccac cactcatcaa cactccggca ctcaggagca gggccaccgg
     1681 agtcaggcgc tgcggacgga gtaccccgaa ctgcgcgata agcgcagcta ctgcggacag
     1741 acccaacagc caggccacct gttggctaat ggcggtggta tccgtgacga actggcgagt
     1801 aagggcaggc gaaagtccaa gctgcatcat gctcaccgcc gcagccagta atattgcgca
     1861 aagcagataa ggcagacaat cccgcttcag actgacgctc ttgcactccg gtgtgggctg
     1921 tggtggtgtg ccgggcaacc gcagcagcat cagcagcgcc agcactggtg ctgccatcag
     1981 tagccccagt ggcgccagag gatgaatggc cagcattgct gccgcgcaca gcggcccgaa
     2041 tagcctgccg caactcaggc cggagctgat ggttgccaga gcggccatgc gattcccttc
     2101 tccagctctc tgcaatgccc agacctgaca ggctggcacc attgccgaca cggtcagccc
     2161 gtaggcgatc cgggcgatga ttaatattcc cagtcccacc gctgttgtta tggcgctggt
     2221 agccatcagc acgcttccca gccctagcag ggtaaagctt atgccatatc ctcccagagc
     2281 ccacaacacc acaggtttac tgccagtacg ggaaatttgc tttccccacc acggggacga
     2341 tggcagaaac agcattgagc caagcatcag cagggcagcc catacagaca gactcagatt
     2401 tgtctgtatg accagaacag ggagcacaac cagcagaccg ttctgtccga taccgagaag
     2461 cccggcactg aacgcaagtg gccagcagga cagtgttttt tggggcgtat cttcgatccc
     2521 tggtgacatt ataatgtttc aactccatgt attaattgtg tttatttgta aaattaattt
     2581 atctgacaat aacatttatt gttgataatg agaatcatta tcgacataat tgttattatt
     2641 ttactgtgtg ggagctgttt gactatgacc ctgccctctg aaaaaccagc cacagatgtg
     2701 gctgcgcagt gcttcctgaa tgcactgatt cgtgaaacca cagactggaa actgacagaa
     2761 tacccgccag acgaattgct catcccgctg gatgagcaga aatcgctcca tttcagagtg
     2821 gcttatttct ccccaaccca gcatcaccgc tttgcatttc ctgcacgtct ggtcacggca
     2881 tcaggcagtt atcctgtcga ctttaccact ctctcccggc ttattattga taagctacgc
     2941 catcaactgt ttctgcccgt tcccctctgc gaaactttcc accagcgcgt gctggaaagc
     3001 catgtccata cgcaacaggc aattgatgcc cgtcatgact gggccgctct gcgtgaaaaa
     3061 gcgttgaatt ttggcgaggc tgagcaggcg ctgctgacag gacacgcttt ccaccctgcg
     3121 cctaagtctc atgaaccgtt taaccgacgg gaggctgaac gctacctgcc tgatatggca
     3181 ccccacttcc cactgcggtg gttttcggtg gataaaacgc aaatcgccgg tgaaagtttg
     3241 catctcaacc ttcaacagcg gctgacgcga tttgccgcag agaatgcgcc tcagttactc
     3301 aacgaattaa gtgacaacca atggctgttc ccgttgcacc cgtggcaggg ggaatatctt
     3361 ttgcagcagg ggtggtgcca ggcacttgtt gctaaagggc tgattaaaga cttaggtgag
     3421 gccggcacgt cgtggctgcc gaccacctct tcccgttccc tctactgtgc caccagccgc
     3481 gatatgatca agttctccct gagcgtacgg ctgaccaact ccatccgtac tctgtccgtg
     3541 aaagaagtga agcgtgggat gcgcctggca cgcctggctc aaaccgacgg ctggcagatg
     3601 ctacaggtcc gcttcccgac tttccgggta atgcaggagg atggctgggc cgggctgctc
     3661 gatcttaacg gcaacatcat gcaggaaagt ctgtttgccc tgcgtgaaaa tctgctggtg
     3721 gatcagccga aaagccagac taacgtactg gtctccctga ctcaggccgc acctgatggc
     3781 ggtgattcgc tgctggtttc ggcggtaaaa cgcctgagcg atcgcctcgg tatcactgtg
     3841 caacaggccg cccatgcatg ggtcgatgca tactgtcagc aagtgctaaa gccgctgttt
     3901 acggctgaag cggattacgg cctggtgctg ctggcgcatc agcaaaatat tcttgtccag
     3961 atgcttgggg atctgccggt cggatttatt taccgtgact gtcagggcag cgcttttatg
     4021 cctcacgcga cagactggct cgattctatt ggcgaggcgc aggcggaaaa tattttcacc
     4081 catgagcagt tgctgcgcta tttcccttat tacctgctgg ttaactccac ttttgctgtg
     4141 accgctgcgc tgggggctgc cgggctggac agcgaatcga atctgatggc tcgtgtacga
     4201 gcatcgctgg ctgaagtgcg tgatcaggtg actcataaaa cctgtctcaa ctacgtgctg
     4261 gaaagtccgt actggaacgt aaaaggtaac tttttctgtt atctgaacga tcataacgag
     4321 aacaccattg ttgacccttc ggtgatctat ttcgatttcg ctaacccgct gcaggctcag
     4381 gaggtctgaa tgtctgaggc aaacattatt cacagcagat atggactgcg ctgtgaaaaa
     4441 ctcgacaagc ccctgaatct tggctgggga ctggacaata gcgcggtgtt gcactgtccc
     4501 ggggagctgc cgacagggtg gctgtgcgat gcgctggatc agatatttat cgccgcacca
     4561 caactgtcag cagtggctct cccctgggcc gaatggcgtg aggagccaca ggcgctgacg
     4621 ctttttgggc aggtaaaaag cgacattatc catcgtactg ccttctggca gttaccgtta
     4681 tggttgagtt caccggcaaa ccgggcctcc ggtgaaatgg tttttgatgc agagcgtgag
     4741 atttatttcc cgcagcgccc accccgtcca cagggtgagg tttaccgccg ttacgatcca
     4801 cgtattcgca ggatgctgag tttccgcatt gccgatcccg tttctgatgc agaacgtttc
     4861 acccgctgga tgaatgatcc gcgcgttgag tatttctggg agcaaagtgg ctcactggag
     4921 gtacagaccg cctatctgga gcgccagctc accggtaaac atgcattccc gctgattggg
     4981 tgcttcgacg atcggccatt cagctatttc gaaatctact gggcggcgga agaccgcatt
     5041 ggtcgtcact attcgtggca gccctttgac cgtggcctgc atctgctggt tggtgaacag
     5101 caatggcgcg gggcccacta tgtgcaaagc tggctgcgcg ggctgacaca ttacctgctg
     5161 ctggatgagc cccgtacgca gcgcacagta ctggagccac gcaccgataa ccagcgcctg
     5221 ttccgccatc ttgagcctgc gggataccgg acaattaaag agttcgactt cccacataag
     5281 cgctcgcgca tggtgatggc ggatcgtcat cacttcttca cggaggtcgg tctgtaatga
     5341 atcacaagga ctgggatttt gttaaccgtc ggctggtggc aaaaatgttg tctgagatgg
     5401 agtatgagca ggttttccac gcggaatctc agggcgatga ccactactgc attaacctgc
     5461 cgggagcaca atggcgcttc atcgctgaac gtggtatctg gggctggctg tggattgatg
     5521 cccaaacgtt gcgctgcacg gacgaaccag tactggcgca gacgctgctg atgcagctaa
     5581 agccggtgct gtcaatgagt gatgccactg ttgcagagca tatgcaggat ttgtatgcca
     5641 cgctactggg cgacctgcaa ctgctgaagg cccgtcgcgg actgagtgcc agtgacctga
     5701 ttaatcttga tgccgatcgt ctgcaatgcc tgctgagcgg tcatcctaaa ttcgttttta
     5761 ataaaggtcg ccgtggctgg ggcaaagagg cgctggaacg atatgcgcca gagtatacca
     5821 acaccttcag actgcactgg ctggcggtaa aacgtgaaca tatgatctgg cgctgcgata
     5881 acgatctgga tattcagcag ttgttgactg ccgcaatgga tccgcaggag tttacacgct
     5941 tcagtcaggt ctggcaggaa aacgggctgg atcacaactg gctaccgctg ccggtacatc
     6001 cgtggcagtg gcagcaaaaa atcgccacag acttcatcgc tgattttgtc gaaggcagga
     6061 tggtgtctct cggcgagttt ggcgaccagt ggctcgccca gcagtcgctg cgtaccctga
     6121 ccaacgccag ccgccgggga gggctggata tcaagctgcc gctgaccatc tacaacacct
     6181 catgctaccg gggaatccct ggcagatata tcgctgccgg accactggct tcacgctggc
     6241 tgcaacaggt ttttgcgacc gacgccaccc tggtgcaaag cggcgcagtg atccttggtg
     6301 aaccggctgc aggctatgtg tcccatgaag gctatgccgc gcttgcccgg gctccctatc
     6361 gctaccagga aatgcttggt gttatctggc gggagaatcc gtgccgctgg ctgaaaccgg
     6421 atgaaagtcc tgttctgatg gcaacactga tggagtgcga cgaaaacaat cagccgctgg
     6481 caggcgcata tatcgaccgc tccggactgg acgctgaaac ctggcttacg caattgttcc
     6541 gggtggttgt ggttccgctg tatcacctgc tttgccgcta cggtgtcgcg cttattgcac
     6601 acggacaaaa tataacgctc gccatgaaag agggggtacc acagcgtgtt ctgctgaaag
     6661 acttccaggg cgatatgcgg ctggtgaaag aagcgtttcc cgaaatggac tctttgcctc
     6721 aggaggtgcg tgatgccaca tcccgtctga gtgcggacta cttaatccat gatttgcaga
     6781 cgggtcactt cgtgacagta ctgcgtttta tttcgccact gatggttcgt cttggcgtac
     6841 ctgaaaggcg attttatcaa ctgctggcag cagtgttgag tgattacatg aacaaacatc
     6901 cacaaatggc agagcgtttt gcgcttttct cactcttcag gccacaaatc attcgcgtgg
     6961 tgctgaaccc ggtaaaactg acctggccgg acctggatgg cggcagtcgc atgctgccga
     7021 attaccttga gaatctgcaa aatccgctgt ggctggtaac tcaggaatat gaatcatgaa
     7081 aaaaagtgtc gattttattg gtgtcgggac agggccattt aatctcagca ttgctgcatt
     7141 gtcacatcag atcgaagaac tgaactgtct cttctttgac gaacatcctc atttttcctg
     7201 gcatccgggt atgctggtac cggattgtca tatgcagacc gtctttctga aagatctggt
     7261 cagtgcagtt gcacctacaa atccctacag ctttgttaac tatctggtga agcacaaaaa
     7321 gttctatcgc ttccttacaa gcaggctacg tacagtatcc cgtgaagagt tttctgacta
     7381 tctccgctgg gctgctgaag atatgaataa cctgtatttc agtcataccg ttgaaaatat
     7441 tgatttcgac aaaaaaagtc gattgtttct ggttcagacc agtcggggag aatattttgc
     7501 ccgcaatatc tgccttggta caggaaaaca accttattta ccaccctgtg tgaagcatgt
     7561 gacacaatcc tgtttccatg ccagtgaaat gaatcttcgt cggccggacc tgagtggaaa
     7621 acggataacc gtggttggtg gaggacagag tggtgcagac ctgttcctta atgcattacg
     7681 cggggaatgg ggagaagcgg cggaaataaa ctgggtctca cggcgtaata attttaacgc
     7741 actggatgag gctgcttttg ctgatgagta ttttacacct gaatatattt caggcttctc
     7801 cggactgaag gaagatattc gccatcagtt actggatgag cagaaaatga catcggatgg
     7861 catcactgcc gattctttac tgaccattta tcgtgagttg taccaccgtt ttgaagttct
     7921 gagaaaacca agaaatatcc gtctgctacc cagtcgctcg gtaacaaccc tggaaagtag
     7981 tggtccgggc tggaggttat tgatggaaca tcatctggat cagggcagag agagcctgga
     8041 aagtgatgtg gtgattttcg ccacaggtta ccgttctgca ttgccacaaa tacttccctc
     8101 actgatgccc ttgatcacca tgcacgataa gaacaccttt aaagtgcgtg atgacttcac
     8161 tctggaatgg agtggcccga aagaaaataa catctttgcg gtcaacgcca gcatgcaaac
     8221 tcatggcatc gccgaacccc agctcagcct gatggcctgg cgatctgcac gtattcttaa
     8281 tcgcgtactg gggcgtgatt tattcgatct cagtatgccg cccgcactga ttcagtggcg
     8341 cagcggcagc cggaaaaaac cgcagccgga ggctgcttct ttaactcact atacagcaaa
     8401 tattcaggaa taacgatgat gcgcaaaaag tatatgcccc gggctcttgg tccgctgctt
     8461 cttgtcgtgc tgtcaccagc tgtcgcccag caaaacgatg ataatgagat catagtgtct
     8521 gccagccgca gcaatcgaac tgtagcggag atggcgcaaa ccacctgggt tatcgaaaat
     8581 gccgaactgg agcagcagat tcagggcggt aaagagctga aagacgcact ggctcagtta
     8641 atccccggcc ttgatgtcag cagccagagt cgaaccaact acggtatgaa catgcgtggc
     8701 cgcccgctgg ttgtcctgat tgacggtgtg cgcctcaact cttcacgttc cgacagccga
     8761 caactggact ctgtcgatcc ttttaatatc gaccatattg aagtgatctc cggcgcgacg
     8821 gccctgtacg gtggcgggag taccggaggg ttgatcaaca tcgtgaccaa aaaaggtcag
     8881 ccggaaacca tgatggagtt tgaggctggc acaaaaagtg gctttaacag cagtaaagat
     8941 cacgatgagc gcattgccgg tgctgtctcc ggcggaaatg accatatctc cggacgtctt
     9001 tccgtggcat atcagaaatt tggcggctgg tttgacggta acggcgatgc caccctgctt
     9061 gataacaccc agaccggcct gcagcactcc aatcggctgg acatcatggg aaccggtacg
     9121 ctgaacatcg atgaatcccg gcagcttcaa ctgataacgc agtactataa aagtcaggga
     9181 gacgacaatt acgggcttaa tctcgggaaa ggcttttccg ccatcagcgg gagcagcaca
     9241 ccatacgtca gtaaggggct gaattctgac cgcattcccg gcactgagcg gcatttgatc
     9301 agcctgcagt actctgacag tgatttcctg ggacaggaac tggtcggtca ggtttactac
     9361 cgcgatgagt cgttgcggta ctacccgttc ccgacggtaa atgcgaataa acaggcgaca
     9421 gctttctcct cgtcacagca ggataccgac cagtacggca tgaaactgac tctgaacagc
     9481 caactgatgg acggctggca aatcacctgg gggctggatg ctgagcatga gcgctttacc
     9541 tccaaccaga tgttcttcga tctggctcag gcaagtgctt ccggagggct gaacaaccat
     9601 aagatttaca ccaccgggcg ctatccgtca tatgacatca ccaatctggc ggccttcctg
     9661 caatccagct atgacattaa tgatattttt accgttagcg gtggcgtacg ctatcagtat
     9721 actgagaaca gggtagatga tttcatcgac tacacgcagc aacagaagat tgctgccggg
     9781 aaggcgatat ctgccgacgc cattcctggt ggttcggtag attacgataa ctttctgttc
     9841 aatgctggtc tgctgatgca catcaccgaa cgtcagcagg catggttcaa tttttcccag
     9901 ggggtggcat tgccggatcc ggggaaatat tatggtcgcg gcatctatgg tgcagcagtg
     9961 aacggccatc tttccctgac aaagagcgtg aacgtcagcg acagtaagct ggaaggcgtg
    10021 aaagtcgatt cttatgaact gggctggcgc tttaccggtg acaacctgcg gactcaaatc
    10081 gcggcatatt actcgctttc caataagagc gtggaaagga ataaagatct gaccatcagt
    10141 gtgaaggacg acaggcgccg tatttacggc gtggaaggtg cggtggacta cctgatcccg
    10201 gatactgact ggagtaccgg tgtgaacttc aatgtgctga aaaccgagtc gaaagtgaac
    10261 ggtcaatggc aaaaatatga cgtgaaggaa tcaagtccat cgaaagcgac agcttacatt
    10321 aactgggcgc cggaaccgtg gagtctgcgt gtacagagca ccacttcttt cgacgtaagc
    10381 gatgcagagg gtaacgatat taatggttac actaccgtcg attttatcag tagttggcag
    10441 cttccggtgg gaacactcag cttcagcgtt gagaacctct tcgaccgtga ctataccact
    10501 gtctggggac agcgtgcacc tctgtactac tgcccgggtt acggccctgc ttcactgtac
    10561 gactacaaag gccggggccg aacctttggt ctgaactact cagtgctgtt ctgacgggga
    10621 tgcttttgca acaagggtat gctgtaaaca acatggtcct tggtggccat gttgtttaca
    10681 gccagtataa tatacagcct taacaagata ttccgtctcc ggaatggact gttcaccact
    10741 gaataacatt atttctgttt tatggatctt tgactgaaat ttatcgcata tcctcatttc
    10801 attgtccgtc tgaaaactaa ccttacaagt aagcgtcgtc tcagcaccgt ctggcagatc
    10861 ctgaaattcc tgagaggatg atctgcgccc aatatcctga gccggaactt tgctgcaatg
    10921 aaggccaacg aaaaatgggg gagtatgtta tagagtactt gctacagggt aaaaagaggt
    10981 acctgtcgct ggttctccgt tcagtttaac cgggagattt tctcctgcgc ctgtcataaa
    11041 gtccggtaat ggagattgtt aatacgatgg tgcacgatgc attgtcggtg cccggcccgg
    11101 aggatgcccc gtaactacat acggatcagg gttggcaata tcgaatggca ggctataggc
    11161 aaagttaaag ccccatggag tagcacaaaa tatgccacac aaaggaaacg gtctgaataa
    11221 cgcagtgatg gagaacttct tcggcacact gcaaaatcgc atcgtggtcg atcgctaatt
    11281 cgccgtgaat cactgaacga tgttccatgc gaacagatat tcgatcatat cgaaatatta
    11341 aacattaccc ctccccgcat tgggcaaaaa aaaaacaaaa aacgatcaat tagtattgat
    11401 tcacataatc tctcatcatt aatgagaaca ttgtcacata tttacaatat agaactgtta
    11461 cttttagtaa gctgttagta accaactgtt actaataatt ttaattctta atactttgtt
    11521 cattcaataa atgagttgag ag
//
LOCUS       DAEEKQ010000123        11428 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.124, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000123 DAEEKQ010000000
VERSION     DAEEKQ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11428)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11428)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11428
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.124"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(42..383)
                     /gene="bamE"
                     /locus_tag="KLK57_003069"
     CDS             complement(42..383)
                     /gene="bamE"
                     /locus_tag="KLK57_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HBE0893374.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(532..2193)
                     /gene="recN"
                     /locus_tag="KLK57_003070"
     CDS             complement(532..2193)
                     /gene="recN"
                     /locus_tag="KLK57_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HBE0893375.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="KLK57_003071"
     CDS             complement(2279..3157)
                     /gene="nadK"
                     /locus_tag="KLK57_003071"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HBE0893376.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            3280..3873
                     /gene="grpE"
                     /locus_tag="KLK57_003072"
     CDS             3280..3873
                     /gene="grpE"
                     /locus_tag="KLK57_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HBE0893377.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            complement(3928..5214)
                     /locus_tag="KLK57_003073"
     CDS             complement(3928..5214)
                     /locus_tag="KLK57_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HBE0893378.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(5235..6026)
                     /locus_tag="KLK57_003074"
     CDS             complement(5235..6026)
                     /locus_tag="KLK57_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HBE0893379.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            6193..7554
                     /gene="ffh"
                     /locus_tag="KLK57_003075"
     CDS             6193..7554
                     /gene="ffh"
                     /locus_tag="KLK57_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HBE0893380.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            7803..8051
                     /gene="rpsP"
                     /locus_tag="KLK57_003076"
     CDS             7803..8051
                     /gene="rpsP"
                     /locus_tag="KLK57_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HBE0893381.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            8070..8618
                     /gene="rimM"
                     /locus_tag="KLK57_003077"
     CDS             8070..8618
                     /gene="rimM"
                     /locus_tag="KLK57_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HBE0893382.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            8649..9416
                     /gene="trmD"
                     /locus_tag="KLK57_003078"
     CDS             8649..9416
                     /gene="trmD"
                     /locus_tag="KLK57_003078"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HBE0893383.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            9458..9805
                     /gene="rplS"
                     /locus_tag="KLK57_003079"
     CDS             9458..9805
                     /gene="rplS"
                     /locus_tag="KLK57_003079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HBE0893384.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(9882..10364)
                     /locus_tag="KLK57_003080"
     CDS             complement(9882..10364)
                     /locus_tag="KLK57_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HBE0893385.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="KLK57_003081"
     CDS             complement(10380..>11428)
                     /gene="dgcN"
                     /locus_tag="KLK57_003081"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HBE0893386.1"
                     /translation="TYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNILAS
                     WHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSLAV
                     LTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFHRF
                     ALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDARK
                     TSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEVGGLRHKAYRLGGDEFAMVLY
                     DVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQELADHN
                     MYQAKHQRAEKLVR"
BASE COUNT         2803 a         2747 c         3061 g         2817 t
ORIGIN      
        1 attgagcacc ttttttaacg tctttgagag caactttatt attagttacc actcagcgca
       61 ggtttgttat caatattggt caacacaccg ctactgttaa aggtcagcgt cagcgtttgc
      121 tgcgttacac cttcatgacc tggttgctgg cggaagacat agaaccaggt attcgtacca
      181 aatggatcgg acatcagcgg tgtacccaat gcgtacgcaa cttgttgttg cgtcatgcca
      241 acacgtattt tggatacgtc gttagcggtc agatagttcc cctggttgat gtcaggacgg
      301 taaaccactc gctccagagt ggaacagcct gcggtcaaca tcaatagtac tgctgctgca
      361 gcagtcagcg ttttacagcg catagtgatt tgattccttt tcgggcccga gcagtacgtg
      421 gctcatctgt aatatgccga tgataataga cctttcatca ctttaaaacc ttttgctttc
      481 cggtcttacg gcgttttgct gtttactcta accgtgaagc aggaaaaaag tttacgctgc
      541 aagcagttct ttcgcattcg ctagtgtatt acgtgtgact tcactgccac caagcaggcg
      601 cgccagctct tgtaaccgcg cttttttatt cagggattgc atatgcgttt ctgtcatcgc
      661 accatcggtt tctttgctga caaaatagtg ttgatgacca catcccgcga cttgtggcag
      721 gtgggtaaca cacatcacct gagttgattc gccgagttga cgcagcagtt tgccgacaac
      781 tgccgctgtt ggaccgctaa tccctacatc cacttcatca aaaatcagtg ccggggtttc
      841 cattttacgc gccgtgatga cctggattgc cagtgcgatg cgggacaatt caccaccgga
      901 tgcgactttg gcaataggct gcattggctg acctgggttg gtggttaccc gaaactcaat
      961 acgatcggca ccgtcagcgc ccaggtgatg ctcgtcaaat ttaacatcga tcgtaaactg
     1021 cccatgcggc attgagagcg catgcatact gtcggtgatc agctgtgcaa gttcttctgc
     1081 atattgctgg cgttgttggt gtaatgcgcg cgcgatttcc agtgcctgct gatgatgttt
     1141 cgttaccgcc agcgcaagcg tttcttgtga gtcggcctga tcgtccagtt gctgctgttc
     1201 ttccagtagc gactggtaat actgtggcaa tgcctcaggg ctgacgtgat gtttacgtgc
     1261 cagcgaaatc tgttttgaga tgcgctgttc aagttcaaat agtcggttgg gatcgagatc
     1321 cagacgatcg cagtagtggc gcagttcatc gctggcttca gcaatctgga tggtagcttc
     1381 ttccagcata tcaagtacgc cggacagttt gctgtccatg ccaatcaatt cgctcaccag
     1441 ttgtttagcc gtgtaaagct gactttgcag gtttgcgtct tcaccgtcgg ccattaatgc
     1501 caatgcattc tggctggtgg tcagcaattg accgctgttc gccagacgtt tgtactcttc
     1561 gtcgatttgt tcaaactctc cgggctgcgg attaaattcg ttaagttctt ttaattggta
     1621 ttgcagcagt tccgcacggg cggcgcgttc ctgacttaac tgttgatgat gcgcgaggtc
     1681 acggcagctt tgatgccaca actgataacg tgcggtcatt tcctgcagta gagaggtttc
     1741 attggcatag ccatcaagca ggaatttttg gtgctcaggt ttggtgagta attgatgagc
     1801 gtgctgacca tggatctgaa tcagcaactg acccagttcg cgcagttgtg acagaggaac
     1861 agctgtaccg ttgatgaaac cacgggagcg accatcgctg ctgatcacgc gacgaagcaa
     1921 acattcatgc ccgtcttcaa gctggttttc ttccagccag cgcagagccg ctggcgtatc
     1981 tttcagagaa aaacgggcgc acaggtcagc gcgagcagcg ccggtacgca ccatgtcggc
     2041 ttcagcgcga ccaccgagac aaagaccgag ggcatctatt gcaatagatt tacccgcgcc
     2101 ggtctcgcca gttattacgg tcatgccgct atgaaaatca atctcaagct cacgaacgat
     2161 agcaaagttg ctgatggtca gttgtgccaa catagtcgct ttcctgtatg aaaaaccatt
     2221 actgttattg tgtacagtat aaactggttt tatatacagt aaagaggctg gcgtaaaatt
     2281 agaataattt ttttgaccag ccgagcttgg tgcttaatgt gttgaaataa ctgtaatctt
     2341 tcggatgaat cagattcaga tggtaatcac agcgacgaat caggacatct tcaccttcct
     2401 gaatcggcag tgctatctgg ctgtcgcaac tgatttccag gtcgttacgg cgatgcgaaa
     2461 aacgcagacg gatcgtgctg ctgctgttta tgaccagtgg tcgtgctgac aacgtatgcg
     2521 ggaacatggg caccagggta atcgcatcca gagagggggt cagaatagga ccgcctgcag
     2581 agagggaata ggcggtggag cctgttggcg tcgaaataat tagtccatca gatcgctgag
     2641 aaaacgcaaa gatctcgtcg atatacactt cgaactcaat catatgcgcc actttgcctg
     2701 gatgaagtac cacttcattt atcgcggtgc tgatgcgttt ctggcaatct tgctgacaga
     2761 cttgcgcttc cagcaaaaaa cgtttctcgc tgatgtagtg gccttccagc acatcggcta
     2821 actgttgctg ggcgttatcg gggtcaaggt cagtcaggaa acccaggttg ccacggttga
     2881 ttccaataac tttaatatcg taacgggcga gtgtgcgtgc cgcgcccagc atattaccgt
     2941 cgccaccaac gactaccgcg agatcagcta gttgcccaat ctccgcgagc gtgccagttt
     3001 tcacattctt cagttgcagt tcgtgagcga tttgttgctc aacgatgacc tcgtaacctt
     3061 ttgtgcacag ccagcggtag agcatttcat gtgttgtcag tgcagtgggg tgccgtgggt
     3121 gtcccacaat gccaatacac ttgaaatgat tattcatttt tccgaggtcc ttgttgcgaa
     3181 gattgatgac aatgtgagtg cttcccttga aaccctgaaa ctgatcccca taataagcga
     3241 agttagcgag atgaatgcga aaaaaacgcg gagaaattca tgagtagtaa agaacagaaa
     3301 acgcctgagg ggcaagcccc ggaagaaatt atcatggatc agcacgaaga gattgaggca
     3361 gttgagccag aagcttctgc tgagcaggtg gatccgcgcg atgaaaaaat tgcgaatctc
     3421 gaagctcagc tggctgaagc ccagacccgt gaacgtgacg gcattttgcg tgtaaaagcc
     3481 gaaatggaaa acctgcgtcg acgtactgaa ctggatattg aaaaagccca caaattcgcg
     3541 ctggagaaat tcatcaacga attgctgccg gtgattgata gcctggatcg tgcgctggaa
     3601 gtggctgata aagctaaccc ggatatgtct gcgatggttg aaggcattga gctgacgctg
     3661 aagtcgatgc tggatgttgt gcgtaagttt ggtgttgaag tgatcgccga aactaacgtg
     3721 ccgctggacc cgaatgtgca tcaggctatc gcaatggtgg aatctgatga cgttgcgcca
     3781 ggtaacgtac tgggcattat gcagaagggt tatacgctga atggtcgtac gattcgtgcg
     3841 gcgatggtaa ctgtagcgaa agcaaaagcg taatttctgt tttcgtaata attcacggcc
     3901 ctgcatgcga atgcagggcc gttttcgtta ctccgccaca ctctcgcgca gcggtttcac
     3961 aggaaaaact tttacctgct taatcatatt gtcctgtacg tcgagaatat ctatatcgta
     4021 ctcgccaata cgcacgcggg tgcctgcgac agggatctct tccagtgcct caagaatgac
     4081 gccattaacc gtgcgggcat catcttccgg tagatgccag ttaaaggctt tgttgatttc
     4141 ccgcacgttg gcggtgccat cgataatcac cgaaccgtcg ttttgcggcg tgacctcttc
     4201 ggcaagtgtt ggcgacatcg acgtagtgaa atcgccgaca atctcttcca gaatatcttc
     4261 aaccgtcacc agcccctgaa tgtctccata ctcgttgacg accaggccga ctttcttttt
     4321 gttgcgctga aactttacca actgcgtgct gagcggcgta ccttccggca caaaatagat
     4381 ctcgtccgcg gcgcgcagca tggtttcttt ggtgaactct tttttctccg acatcaaccg
     4441 ccaggcttca cgtactcgca gcatactgat ggcgtcgtcc agcgaatcac ggtagagcac
     4501 gatgcgcccg tgaggtgagt gggagagttg gcgcagaatc gatttccagt catcgttgat
     4561 atcaataccg ataatttcac tgcgcggcac catgatgtca tcaacggtca ttttttccag
     4621 atcgagcacc gacagcagca tatcctgatt gcgacgggaa atttgtgagc gtgattcgtg
     4681 cacgatagtg cgcaactctt ctttgctcaa agagccgcta accacgatat cggttttgat
     4741 acccatcatg cgcatcagca tacgggtgat agcattcagc aaccagacca gcggcatcat
     4801 caaaatttgc agcggagcca gcagaaaact actcggataa gcgacttttt ccgggtacag
     4861 cgcggcaatg gttttcggca atacctcagc aaataccagt acgacaaaag tcagcacacc
     4921 agtcgcaatt gccacgcccg catcgccgta caaacgcatc ccaacaatag tgccgagcgc
     4981 ggaggcaaga atattgacca ggttattgcc gattaacacc aggcttatca ggcggtctgg
     5041 cttacgcagc aatttttcga cgcgtttggc cgagcgatta ccctgtttcg ccatatgtcg
     5101 cagacgatag cggttgaggg tcatcattcc ggtttcggac ccggaaaaat aggctgaaat
     5161 gaccaccatg atgatcagaa taatgatcaa cgtagtagta gaaatgtgtt ccaggggaaa
     5221 ctccttttct gggtttagct gattaactgc tggacaattc ggctgccgaa gtaggccagt
     5281 gtcagaatta ccgcgcccgc aacgttaaac cagacgacgc ggcgtccacg ccatccttca
     5341 tggtaatgtc cccacagcag cacaatatag acaaaccacg ccacgataga gagcacagcc
     5401 ttgtcgatat tttccatgct aaataggttg tgcatgtaga acaggccagt gcaaagcgtg
     5461 agcgttagca gcaccacgcc aatctgcgtg atgtggaaca ttttacgctc gatactcatc
     5521 aatggcggca tttcctggtt aaatgccagc ttcttgttct tcagttggta atcaatccac
     5581 gccagttgca gcgcgtacag ggcggcgata attagcgtgg cataggaaaa gagcgataag
     5641 ccaatgtgca ccagcatccc aggcgttgct tccagatggg tgatgtattc attgggcatg
     5701 aaggtcgcca gcgccaggtt gataagcgca aaggcataga caatgggtag cagcaaccag
     5761 ccacgattgc gagaagccac aatggtcatt accgtacaga tcatcaaact gaccaatgaa
     5821 ccaacgttca gcaggctgag gttttgtccg ctatcaccgt cgggcaggat gcgggcttcc
     5881 agagcgattg cgtggcagac cagcgcaatg accgcagaaa taatagccat gcgccgccag
     5941 ccgccgtttt tttgcagcag accgggaaca atcagcgcaa gactgacgga gtaggcgaca
     6001 agcgcgagca gagcaaaaac gggcatagtg atgtcgacag tttgagcaat aagaaagaga
     6061 agcagtatag cgtcaggtga acgctgctcc aaccgttgca taacaacaaa gacgccttca
     6121 tgttatactg cggcaaaata ctgatgatgt gtcgcgattg cggccaaccg tttccacccc
     6181 aggcgagaga caatgtttga taatttaacc gatcgtttgt cgcgcacgct gcgcaatatc
     6241 agtggccgtg gacgcctcac tgaagacaac gtaaaagata cgctgcgcga agtgcgcatg
     6301 gcgctgctgg aggcggatgt cgctctgccg gtagtgcgtg agtttatcaa tcgcgtaaaa
     6361 gagaaagcgg ttggtcatga agttaataag agcctgacgc cggggcagga gttcgtcaaa
     6421 atcgtccgta acgaactggt tgcggcgatg ggcgaagaga accagaccct gaacctggcg
     6481 gcgcaaccgc ctgcggtcgt gctgatggcg ggcctgcaag gtgccggtaa aacaaccagc
     6541 gttggtaaac tcggtaagtt cctgcgcgag aagcacaaga agaaagtgct ggtggtttct
     6601 gccgacgttt atcgcccggc ggcaatcaaa cagcttgaga cgctggcaga gcaggtgggc
     6661 gttgatttct tcccttctga tgttggtcag aagccggtag atatcgttaa cgcggcgctg
     6721 aaagaagcca aactgaaatt ctacgacgtg ctgctggtgg ataccgctgg tcgtctgcac
     6781 gttgacgaag cgatgatgga cgagatcaaa caagtccatg cgtcgattaa cccggttgaa
     6841 accctgtttg tggttgacgc catgaccggt caggatgcgg ccaatacggc aaaagcattc
     6901 aatgaagcgt taccgcttac cggcgtagtg ttgaccaaag tggacggcga tgcccgcggc
     6961 ggtgcggcgc tctctattcg tcacatcact ggcaaaccga tcaagtttct cggtgttggc
     7021 gagaaaactg aggcgctgga gccgttccat ccggaccgca tcgcgtcgcg tattctcggc
     7081 atgggcgacg tactgtcgct gatcgaagat atcgaaagca aagttgaccg cgcgcaggca
     7141 gagaaattag ccagcaagct gaaaaaaggc gacggcttcg atctcaacga ttttcttgag
     7201 cagctgcgcc agatgaaaaa tatgggcggc atggctagcc tgatgggcaa gctgccgggc
     7261 atggggcaga tcccggataa tgttaagtcg cagatggacg ataaagtgct ggtgcgtatg
     7321 gaagccatca tcaactcgat gacgatgaaa gagcgcgcta agccagaaat catcaaaggt
     7381 tcgcgtaagc gtcgtatcgc tgccggttgc ggtatgcagg tgcaggacgt taaccgcctt
     7441 ctgaagcagt tcgacgacat gcagcgcatg atgaagaaaa tgaagaaggg cggaatggcg
     7501 aagatgatga gaagcatgaa gggtatgatg cccccaggct tccctggtcg ctaatcggcc
     7561 tggcttgttt gccattttgg caccgggatg tgctcgtcat cctcacgtac tgcgtgtacg
     7621 ctccggtgac tgcgcgcact ccggcaccaa actggctgca ccgccgacgg ccttttacag
     7681 cagagttagc aactgttgat tgcaattccc gcaaaaatga gtaaaatttt cgggctttta
     7741 atatgacacc ggactccgtt cctcgatggg gtccggttgt tttattcaca caagaggatg
     7801 ttatggtaac tattcgttta gcacgtcacg gcgctaaaaa gcgtccgttc taccaggttg
     7861 ttgtcgctga cagccgtaat gcacgcaacg gtcgcttcat cgagcgcgtt ggtttcttca
     7921 acccaatcgc tagcgaaaaa gaagaaggca ctcgcctgga tctggatcgc atcgctcact
     7981 gggttggcca gggcgcaact atttctgatc gcgttgctgc gctgatcaaa gaagtaaaca
     8041 aagcagctta atctgtcacg gtggtcatga tgagcaaaca actcaccgcg caagcacctg
     8101 ttgatcccat cgttttggga aaaatgggtt cgtcttacgg tattcgtggg tggctcagag
     8161 tgttttcttc caccgaagac gccgaaagca tttttgacta tcagccctgg tttatccaga
     8221 aggcgggtca gtggcagcaa gtccagctgg aaagctggaa gcaccacaat caggacatga
     8281 tcatcaagct gaaaggcgtt gacgatcgtg atgcggcgaa cctgctgacg aattgtgaaa
     8341 ttgtcgtgga ttcatcgcag ctgcctcagc ttgaagaggg ggactactac tggaaagacc
     8401 tgatgggctg ccaggtagta accactgaag gctacgatct cggtaaagtc gtcgatatga
     8461 tggaaaccgg atctaatgac gttctcgtca ttaaggcaaa cctgaaagat gcgtttggta
     8521 tcaaggaacg tctcgtaccg ttcctcgatg ggcaggttat caagaaagtc gatctcacta
     8581 ctcgttcaat cgaagtagat tgggatcctg gtttttaaac caccggataa acggtaaaag
     8641 acggcgctat gtggattggc ataattagcc tgtttcctga aatgttccgc gcaattaccg
     8701 attacggggt aactggccgg gcagttaaaa atggcctgct gagcatccag agttggagtc
     8761 ctcgcgactt cacgcatgac cggcaccgta ccgtggacga tcgtccttac ggtggcggac
     8821 cggggatgtt aatgatggtg caacccttgc gggacgccat tcatgcagca aaagccgcgg
     8881 cgggtgaagg cgcaaaggtg atttatctgt caccacaggg acgcaagctt gatcaagcgg
     8941 gcgtcagcga actggcaacg aatcaaaaat tgattctggt gtgcggtcgc tacgaaggta
     9001 tagatgagcg cgtgatccaa accgaaattg acgaagaatg gtcaatcggc gattacgttc
     9061 tcagtggtgg tgagttacca gcaatgacgc tgattgactc cgtttcccgg tttattccgg
     9121 gagtactggg acatgaagcc tcggcaacgg aagattcctt tgctgaagga ttgctggatt
     9181 gcccgcacta tacgcggcct gaggtgttag aagggatgga agttccgcca gtgttactgt
     9241 cgggcaacca tgccgagata cgtcgctggc gtttgaaaca gtcgctgggc cgtacctggc
     9301 ttagaagacc tgaacttctg gaaaacctgg ctctgactga agagcaagca aggttgctgg
     9361 cggagttcaa aacggaacac gcacaacagc aacataaaca tgatgggatg gcgtaagccc
     9421 ccgagatatc agtttaccca ggataagaga ttaaattatg agcaatatta ttaagcaact
     9481 tgaacaagag cagatgaagc aggacgtacc ttccttccgt ccgggtgata ccgtggaagt
     9541 gaaagtatgg gttgttgaag gttccaaaaa acgtctgcag gcattcgagg gcgtggttat
     9601 cgctattcgt aaccgcggtc tgcactctgc attcactgtt cgtaaaattt ccaacggcga
     9661 aggcgttgag cgtgtcttcc agactcactc tccggtagtt gacagcattt ctgtcaaacg
     9721 tcgtggtgct gttcgtaaag ctaaactgta ctacctgcgt gagcgtactg gtaaggctgc
     9781 tcgtatcaaa gagcgtctta actaagattt cgcttaagcg acatcctgtt aagaagggct
     9841 ggccaattgg ctggcccttt ttttgtctgt ttgctcgtgt tttaaggggt tgtaatcacc
     9901 actgcgacgc ggcggttctc ggcgcggccc tgggcggtct tgttactggc tatgggatat
     9961 ttttttccta aaccctgtgt ggtgagattg ctgcgtggaa tttgtccacc aatagcccat
    10021 gcatcggcca cgacattcgc ccgtttcaat gataagcctt cgttgtaact gtcttcacca
    10081 tagttatcgg tgtgtccatc catacgggca tgtgttagcc ctgtcgaggc caatttggct
    10141 gccatggttt ggatctgttg ctggctttcc gggagcaatt tgtagtcatt ttttgcgaac
    10201 agaatggcat ctgataagcc cagcgaccag tcgccggcgg attcagtaaa tccataagat
    10261 tgcatagcgg cgacttgctc aggagtaaac tttccctgag gggactggca gccagtcagt
    10321 attagtgagg tgaaaatcag gggtgctact aggtgcttta tcatatcgat atatccttgt
    10381 tatctcacca gcttttcggc acgctggtgt ttggcctgat acatattgtg atcggcaagc
    10441 tcttgtaatt tttccgcaga ggcgtgctca atggtcatcg cgtaaccaat gcttaatgtc
    10501 atggtggtct gatggccatt atgaagatca aacgggagat taaagatttg tgtcagtgct
    10561 gagcatatct gctgcacttc agattctgac tgtacatcat agagcaccat agcgaattca
    10621 tcgccgccca ggcggtatgc tttatgtcgc agcccgccaa cttcagctaa ccgttttgcg
    10681 atttcaatca agactctatc gcccgtcgca tgaccccagg tatcattgat gtatttgaaa
    10741 ttatcgccat caagaaatag taacgccgac gtttttcggg catcggaatt gttcatcaac
    10801 gtgttgatgc cgctacgaaa cgctgcgcgg ttagccagcc cggttaatgg gtcatgtagc
    10861 gcggtacgta gaagctgcgc atttttagcc tgtaaacgaa gctgccactc ttccatttca
    10921 tccagcagac tgttgaagtc gagagcgaag cggtgaaact cagcgatacg ttcttccgaa
    10981 actcgtcggg aaaaattgcg gttggaacgc acatcatgta cgacatcggt gatatttttc
    11041 agtgcttcca ccaggccatt gtgcaaatgg cgggtgaggg taattgcgat gcctgatgcc
    11101 agcagaatac aaccggtcag tacggcgagc gaaaaccaga taaaatggct gattgaactg
    11161 tcgcgagcgg ttaagcgtac ttcgccaatg gtttcaccat tgtgacgaat cggctgaatg
    11221 atgggggcgg ggaagagcca gtggcttatg aaattgctga aagtatcgcc tggatcctta
    11281 cgggtgtaat gccaggacgc cagaatattt tgctgcttat cacgtacttc tgcagttgaa
    11341 aattgccctt gctggcccag cgctgccagt gtttcagttg ctgcagggcc atcggcaaaa
    11401 acgacagctg cttccagact gtaagtca
//
LOCUS       DAEEKQ010000124        11355 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.125, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000124 DAEEKQ010000000
VERSION     DAEEKQ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11355
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.125"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..535)
                     /gene="espL1"
                     /locus_tag="KLK57_003082"
     CDS             complement(<1..535)
                     /gene="espL1"
                     /locus_tag="KLK57_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
                     /protein_id="HBE0893387.1"
                     /translation="MSQNDIIIRTHYKSPHRLHIDSDIPTPSSEPINQFARQLITLLD
                     TSDLSSMLSYCVTQEFTANCRKISQNCYSTALFIINFATSPIHAENILITLHYKKEII
                     PLFLETTPIKANHLRSILDYIEQEQLTAEDRNHCMKLSKKIHREKTIQPTVNLNGSAF
                     FSQSPSDAIFCRHLSLQY"
     gene            1060..2988
                     /gene="thrS"
                     /locus_tag="KLK57_003083"
     CDS             1060..2988
                     /gene="thrS"
                     /locus_tag="KLK57_003083"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HBE0893388.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2992..3534
                     /gene="infC"
                     /locus_tag="KLK57_003084"
     CDS             2992..3534
                     /gene="infC"
                     /locus_tag="KLK57_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HBE0893389.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3631..3828
                     /gene="rpmI"
                     /locus_tag="KLK57_003085"
     CDS             3631..3828
                     /gene="rpmI"
                     /locus_tag="KLK57_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HBE0893390.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3881..4237
                     /gene="rplT"
                     /locus_tag="KLK57_003086"
     CDS             3881..4237
                     /gene="rplT"
                     /locus_tag="KLK57_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HBE0893391.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    4354..4478
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            4360..4404
                     /gene="pheM"
                     /locus_tag="KLK57_003087"
     CDS             4360..4404
                     /gene="pheM"
                     /locus_tag="KLK57_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HBE0893392.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4687..5670
                     /gene="pheS"
                     /locus_tag="KLK57_003088"
     CDS             4687..5670
                     /gene="pheS"
                     /locus_tag="KLK57_003088"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HBE0893393.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLLGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5685..8072
                     /gene="pheT"
                     /locus_tag="KLK57_003089"
     CDS             5685..8072
                     /gene="pheT"
                     /locus_tag="KLK57_003089"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HBE0893394.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            8077..8376
                     /gene="ihfA"
                     /locus_tag="KLK57_003090"
     CDS             8077..8376
                     /gene="ihfA"
                     /locus_tag="KLK57_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HBE0893395.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            8477..9457
                     /gene="btuC"
                     /locus_tag="KLK57_003091"
     CDS             8477..9457
                     /gene="btuC"
                     /locus_tag="KLK57_003091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HBE0893396.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASAVLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            9520..10071
                     /gene="btuE"
                     /locus_tag="KLK57_003092"
     CDS             9520..10071
                     /gene="btuE"
                     /locus_tag="KLK57_003092"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HBE0893397.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPENPIVMESIKLALAK"
     gene            10071..10820
                     /gene="btuD"
                     /locus_tag="KLK57_003093"
     CDS             10071..10820
                     /gene="btuD"
                     /locus_tag="KLK57_003093"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HBE0893398.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10898..>11355
                     /locus_tag="KLK57_003094"
     CDS             10898..>11355
                     /locus_tag="KLK57_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBE0893399.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARR"
BASE COUNT         2872 a         2631 c         3056 g         2796 t
ORIGIN      
        1 cgtattgcaa tgacagatgg cgacaaaaaa tagcgtcaga aggagattgc gaaaaaaatg
       61 cactaccatt gagatttact gttggttgta tagttttttc tctatggatt tttttagaca
      121 gtttcataca atggttacga tcttcggcag ttaactgttc ctgttcaata taatccagta
      181 tgcttcgcaa atggttagcc ttaataggcg tggtttccag aaataaggga atgatttctt
      241 ttttatagtg taatgtaatg agtatatttt ctgcatggat gggtgaagtg gcaaagttaa
      301 tgataaaaag ggcagtggaa taacaatttt gtgaaatttt tcgacagttt gcggtaaatt
      361 cctgagtaac acagtatgac agcatcgaac ttaagtcaga ggtatcaagt agggtgatga
      421 gttggcgcgc aaattgatta ataggctctg atgaaggcgt tggtatgtcg ctatcgatgt
      481 gcaatctatg aggagactta taatgggttc tgataatgat atcgttttga ctcataatct
      541 attcgcctta tatatgtctg gaggttttga ggatgatgat taattattat cgccagaacg
      601 aaatgtgcac gaaaaattgc tataagaatt gtgtcacttt tatcattgtg atatgaccac
      661 ttcgtcatta atctcatgag cgtttaaaat actaccgcca aatcacgcaa cgaactttgc
      721 tggattcagt ttatgctgct gtaaatccgc tcgcagtaaa cctttcagac gcaccgtgat
      781 gttatcagtg gttctttaag cgttttgctg gtgtactcac tacaaacgaa ttgcgaatcg
      841 aatcaatgtg aaacggaaag gtacaatctc ccttttttta gtgttaacat cgttcaaccg
      901 ggtggaaggt gagaggttaa tgtctttcat ttgtgtacct gattgcgaac caatttagca
      961 tttgttggct agatggtttc gcaatgaact gttaataaac aaatttttct ttgtatgtga
     1021 tctttcgtgt gggtcaccac tgcaaattaa ggatataaaa tgcctgttat aactcttcct
     1081 gatggcagcc aacgccatta cgaccacgct gtaagcccca tggatgttgc gctggacatt
     1141 ggtccaggtc tggcgaaagc ctgtatcgca gggcgcgtta atggcgaact ggttgatgct
     1201 tgcgatctga ttgaaaacga cgcacaactg tcgatcatta ccgccaaaga cgaagaaggt
     1261 ctggagatca ttcgtcactc ctgtgcgcac ctgttagggc atgcgattaa acaactttgg
     1321 ccgcatacca aaatggcaat cggcccggtt attgacaacg gtttttatta cgacgttgat
     1381 cttgaccgca cgttaaccca ggaagatgtc gaagcactcg agaagcggat gcatgagctt
     1441 gctgagaaaa actacgacgt cattaagaag aaagtcagct ggcacgaagc gcgtgaaact
     1501 ttcgccaacc gtggggagag ctacaaagtc tccattcttg acgaaaacat cgcccatgat
     1561 gacaagccag gtctgtactt ccatgaagaa tatgtcgata tgtgccgcgg tccgcacgta
     1621 ccgaacatgc gtttctgcca tcatttcaaa ctaatgaaaa cggcaggggc ttactggcgt
     1681 ggcgacagca acaacaaaat gttgcaacgt atttacggta cggcgtgggc agacaaaaaa
     1741 gcacttaacg cttacctgca gcgcctggaa gaagccgcga aacgcgacca ccgtaaaatc
     1801 ggtaaacagc tcgacctgta ccatatgcag gaagaagcgc cgggtatggt attctggcac
     1861 aacgacggct ggaccatctt ccgtgaactg gaagtgtttg ttcgttctaa actgaaagag
     1921 taccagtatc aggaagttaa aggtccgttc atgatggacc gtgtcctgtg ggaaaaaacc
     1981 ggtcactggg acaactacaa agatgcaatg ttcaccacat cttctgagaa ccgtgaatac
     2041 tgcattaagc cgatgaactg cccgggtcac gtacaaattt tcaaccaggg gctgaagtct
     2101 tatcgcgatc tgccgctgcg tatggccgag tttggtagct gccaccgtaa cgagccgtca
     2161 ggttcgctgc atggcctgat gcgcgtgcgt ggatttaccc aggatgacgc gcatatcttc
     2221 tgtactgaag aacaaattcg cgatgaagtt aacggatgta tccgtttagt ctatgatatg
     2281 tacagcactt ttggcttcga gaagatcgtc gtcaaactct ccactcgtcc tgaaaaacgt
     2341 attggcagcg acgaaatgtg ggatcgtgct gaggcggacc tggcggttgc gctggaagaa
     2401 aacaacatcc cgtttgaata tcaactgggt gaaggcgctt tctacggtcc gaaaattgaa
     2461 tttaccctgt atgactgcct cgatcgtgca tggcagtgcg gtacagtaca gctggacttc
     2521 tccttgccgt ctcgtctgag cgcctcctat gtgggcgaag acaacgagcg taaggtaccg
     2581 gtaatgattc accgcgcaat tctggggtcg atggaacgtt tcatcggtat cctgaccgaa
     2641 gaattcgctg gtttcttccc gacctggctt gcgccagttc aggttgttat catgaatatt
     2701 accgattcac agtctgaata cgttaacgaa ttgacgcaaa aactatcaaa tgcgggcatt
     2761 cgtgttaaag cagacttgag aaatgagaag attggcttta aaatccgcga gcacactttg
     2821 cgtcgcgtcc catatatgtt ggtctgtggt gataaagagg tggaatcagg caaagttgcc
     2881 gttcgcaccc gccgtggtaa agacctggga agcatggacg taaatgaagt gatcgagaag
     2941 ctgcaacaag agattcgcag ccgcagtctt aaacaattgg aggaataagg tattaaaggc
     3001 ggaaaacgag ttcaaacggc gcgccctaac cgtatcaatg gcgaaattcg cgcccaggaa
     3061 gttcgcttaa caggtctgga aggcgagcag cttggtattg tgagtctgag agaagctctg
     3121 gagaaagcag aagaagccgg agtagactta gtcgagatca gccctaacgc cgagccgccg
     3181 gtttgtcgta taatggatta cggcaaattc ctctatgaaa agagcaagtc ttctaaggaa
     3241 cagaagaaaa agcaaaaagt tatccaggtt aaggaaatta aattccgtcc tggtacagat
     3301 gaaggcgact atcaggtaaa actccgcagc ctgattcgct ttctcgaaga gggtgataaa
     3361 gccaaaatca cgctgcgttt ccgcggtcgt gagatggcgc accagcaaat cggtatggaa
     3421 gtgcttaatc gcgtgaaaga cgatttgcaa gaactggcag tggtcgaatc cttcccaacg
     3481 aagatcgaag gccgccagat gatcatggtg ctcgctccta agaagaaaca gtaaggcctt
     3541 caagtagcac tgtctgtgga gccttcgggc ttcacaggct ttgttcgcct ttgtttcgtt
     3601 tattaacaat gcgaagtgga agttattaaa atgccaaaaa ttaagaccgt acgcggtgct
     3661 gctaagcgct tcaaaaaaac cggtaaaggt ggttttaagc acaagcacgc taacctgcgt
     3721 cacattctga ccaaaaaagc gaccaaacgt aaacgtcacc tgcgtccgaa agccatggtt
     3781 tccaaaggcg atctgggcct ggtaatcgcg tgcctgccgt acgcataagc cgttaacgtt
     3841 tttaactttt taattagaat atagatacag gagagcacat atggctcgcg taaaacgtgg
     3901 tgttattgca cgcgcacgtc acaagaaaat tttgaaacaa gctaaaggct actacggtgc
     3961 gcgttctcgc gtataccgcg ttgccttcca ggctgttatc aaagctggtc agtatgctta
     4021 ccgtgaccgt cgtcaacgta agcgtcagtt ccgtcaactg tggattgcgc gtatcaacgc
     4081 agcagcacgt cagaacggta tttcttacag caaattcatc aatggcctga aaaaagcctc
     4141 tgttgaaatc gaccgtaaga tcctggctga tatcgcagta ttcgacaaag tagcgttcac
     4201 cgctctggtt gaaaaagcga aagcagctct ggcataagcc agttgaaaga gggagctagt
     4261 ctccctcttt tcgtttcaac gccatcaaaa cattgacttt tatcgccgta gccttttcaa
     4321 taaaggtctt ttgaagagta ccaaaaggta acgcaagcaa tgaatgctgc tattttccgc
     4381 ttcttttttt actttagcac ctgaatccag gaggctagcg cgtgagaaga gaaacggaaa
     4441 acagcgcctg aaagcctccc agtggaggct ttttttgtat gcgcgttttg aaaaataaaa
     4501 agccagtcta tcaggcgtaa ttgtcgcagc cagtctggac acggccagcg cggagaaacc
     4561 ggagcgtatt taagtacgtg agaatttcga gcactgcccg ggaccaaaat ggcaagtaaa
     4621 atagcctgat gggataggct ctaagtccaa cgaaccagtg tcaccactga cacaatgagg
     4681 aaaaccatgt cacatctcgc agaactggtt gccagtgcga aggcggccat tagccaggcg
     4741 tcagatgttg ccgcgttaga taatgtgcgc gtcgaatatt tgggtaaaaa agggcactta
     4801 acccttcaga tgacgaccct gcgtgagctg ccgccagaag agcgtccggc agctggtgcg
     4861 gttatcaacg aagcgaaaga gcaggttcag caggcgctga atgcgcgtaa agcggaactg
     4921 gaaagcgcag cgctgaatgc gcgtctggcg gcggaaacga ttgatgtctc cctgctaggt
     4981 cgtcgcattg aaaacggcgg tctgcatccg gttacccgta ccatcgaccg tatcgaaagt
     5041 ttcttcggtg agcttggctt taccgtggca accgggccgg aaatcgaaga cgattatcat
     5101 aacttcgatg ctctgaacat tcctggtcac cacccggcgc gcgctgacca cgacactttc
     5161 tggtttgaca ctacccgcct gctgcgtacc cagacctctg gcgtacagat ccgcaccatg
     5221 aaagcccagc agccaccgat tcgtatcatc gcgcctggcc gcgtttatcg taacgactac
     5281 gaccagactc acacgccgat gttccatcag atggaaggtc tgattgttga taccaacatc
     5341 agctttacca acctgaaagg cactctgcac gatttcctgc gtaacttctt tgaggaagat
     5401 ttgcagattc gcttccgtcc ttcctacttc ccgttcaccg agccttctgc agaagttgac
     5461 gtcatgggta aaaacggtaa atggctggaa gtgctgggtt gcgggatggt gcatccgaac
     5521 gtgctgcgta acgttggcat cgacccggaa gtttactctg gtttcgcctt cgggatgggg
     5581 atggagcgtc tgactatgtt gcgttacggc gtcaccgacc tgcgttcatt cttcgaaaac
     5641 gatctgcgtt tcctcaaaca gtttaaataa ggcaggaata gattatgaaa ttcagtgaac
     5701 tgtggttacg cgaatgggtg aacccggcga ttgatagcga tgcgctggcg aatcaaatca
     5761 ctatggcggg cctggaagtt gacggtgtag aaccggttgc cggtagcttc cacggcgtgg
     5821 tcgttggtga agtggttgag tgtgcgcagc atccgaacgc tgacaaactg cgtgtgacaa
     5881 aagtgaatgt cggcggcgat cgcctgctgg acatcgtctg tggtgcgcca aactgccgtc
     5941 agggcctgcg tgtggcggta gctaccattg gtgctgttct gccgggtgat ttcaaaatta
     6001 aagcggcgaa actgcgtggc gaaccgtctg aagggatgct gtgctccttc tctgaactgg
     6061 gaatttctga cgatcacagc ggcattatcg aactgcctgc agatgcgccg attggcactg
     6121 acatccgcga atacctgaaa ctcgatgaca acaccatcga aatcagcgta acgccaaacc
     6181 gtgccgactg cttaggtatc attggtgttg cgcgtgacgt tgccgtgctg aatcagctgc
     6241 cgctggttga accggaaatc gttccggttg gtgcgaccat cgacgacacg ttgccgatta
     6301 cagtcgaagc gcctgaagcc tgcccgcgct atcttggccg tgtggtaaaa ggcattaacg
     6361 ttaaagcgcc aactccgctg tggatgaaag aaaaactgcg tcgttgcggg atccgttcta
     6421 tcgatgcagt tgttgacgtc accaactatg tactgctcga actgggccag ccgatgcacg
     6481 ctttcgataa agatcgcatt gaaggcggca ttgtggtgcg gatggcaaaa gagggcgaaa
     6541 cgctggtgct gcttgatggc actgaagcga agctgaatgc tgacactctg gtcatcgccg
     6601 accacaacaa ggcgctggcg atgggcggca tctttggtgg cgaacactct ggcgtgaatg
     6661 acgaaacgca aaacgtcttg ctggaatgcg ctttcttcag cccgctgtct atcaccggtc
     6721 gtgctcgtcg tcatggcctg catactgatg cgtctcaccg ttatgagcgt ggcgttgatc
     6781 cggcactgca gcacaaagcg atggaacgtg cgacccgtct gctgatcgac atctgcggtg
     6841 gtgaggctgg tccggtaatt gatatcacca acgaagcaac gctgccgaag cgtgcaacca
     6901 tcactttacg tcgtagcaaa ctggatcgcc tgatcggcca tcatattgct gatgaacagg
     6961 taactgacat tctgcgtcgt ctcggctgcg aagtgaccga aggcaaagac gagtggcagg
     7021 cagttgcgcc gagctggcgt ttcgatatgg agattgaaga agatctggtt gaagaagtcg
     7081 cgcgtgttta cggctacaac aacatcccgg atgagccggt acaggcaagc ctgattatgg
     7141 gtactcaccg tgaagctgac ctgtcgctca agcgcgtgaa aacgctgctc aacgacaaag
     7201 gctatcagga agtgatcacc tacagcttcg ttgatccgaa agtgcagcag atgatccatc
     7261 caggcgttga agccttactg ctgccaagcc cgatctctgt tgaaatgtca gcaatgcgtc
     7321 tttctctgtg gaccggcctg ctggcaaccg tggtgtacaa ccagaaccgt cagcagaacc
     7381 gtgtgcgcat tttcgaaagc ggtctgcgtt tcgtaccaga tactcaggca ccgttgggca
     7441 ttcgtcagga tctgatgtta gccggtgtga tttgcggtaa ccgttacgaa gagcactgga
     7501 acctggcaaa agagaccgtt gatttctatg atttgaaagg cgatcttgaa tccgttctcg
     7561 acctgaccgg taaactgaat gaggttgagt tccgtgcaga agcgaatccg gcactgcatc
     7621 cggggcaatc cgcagcgatt tatctgaaag gggaacgtat tggttttgtt ggagttgttc
     7681 atcctgaact ggaacgtaaa ctggatctta acggtcgcac tctggtgttc gaactggagt
     7741 ggaacaagct cgcagaccgc gtggtgcctc aggcgcgcga gatttctcgc ttcccggcga
     7801 accgtcgtga catcgcggtg gtggtcgcag aaaacgttcc cgcagcggat attttatccg
     7861 aatgtaagaa agttggcgta aatcaggtag ttggcgtaaa cttatttgac gtgtaccgcg
     7921 gtaagggtgt tgcggagggg tataagagcc tcgccataag cctgatcctg caagatacca
     7981 gccgtacact cgaagaagag gagattgccg ctaccgtcgc caaatgtgta gaggcattaa
     8041 aagagcgatt ccaggcatca ttgagggatt gaacctatgg cgcttacaaa agctgaaatg
     8101 tcagaatatc tgtttgataa gcttgggctt agcaagcggg atgccaaaga actggttgaa
     8161 ctgtttttcg aagagatccg tcgcgctctg gaaaacggcg aacaggtgaa actctctggt
     8221 tttggtaact tcgatctgcg tgataagaat caacgcccgg gacgtaaccc gaaaacgggc
     8281 gaggatattc ccattacagc acggcgcgtg gtgaccttca gacccgggca gaagttaaaa
     8341 agccgggtcg aaaacgcttc gcccaaagac gagtaatctg atctaactaa aaaggccgct
     8401 ctgcggcctt ttttcttttc actgtcgaag agtcaccgta aaatcaacgc catgacactt
     8461 cagcagaacg gataccatgc tgacacttgc ccgccaacaa cagcgacaaa atattcgctg
     8521 gttattatgc ctgtcagttt tgatgctgct ggcgcttctc ttaagccttt gcgccggtga
     8581 acaatggatt tcgccaggtg actggtttac tcctcgtggc gaactgttcg tctggcaaat
     8641 tcgcctgcca cgtacgctgg ctgtattgct ggttggtgcg gcgctggcta tatccggcgc
     8701 tgtaatgcag gcgttgtttg aaaatcctct ggcagaacct ggactacttg gcgtctctaa
     8761 cggcgcaggc gtggggctta tcgccgcggt attgcttggg caagggcaac tccccaactg
     8821 ggcgctaggg ctgtgtgcga ttgctggcgc gcttatcatc actttaatac tcttacgttt
     8881 cgcccgtcgt catctttcga ccagtcggtt attgctggct ggcgttgcat tagggattat
     8941 ctgtagcgca ctaatgacgt gggctatcta cttttccacc tctgttgatt tacgtcagct
     9001 gatgtactgg atgatgggcg gttttggcgg cgtagactgg cggcaaagct ggctgatgct
     9061 ggcattgatc cccgtgttgt tgtggatctg ttgtcagtcc aggccgatga atatgttagc
     9121 acttggcgag atctcggcgc ggcaactggg tttacccctg tggttctggc gcaatgtgct
     9181 ggtggcagcg accggctgga tggttggcgt cagtgtggcg ctggcgggtg ctatcggctt
     9241 tattggtctg gtgatcccac atattcttcg gttgtgtggt ttaaccgatc atcgcgtatt
     9301 acttcccggc tgcgcgctgg caggggcgag cgcagtgctg ctggccgata ttgtagcgcg
     9361 cctggcatta gctgccgcag agctgcctat tggcgtggtc accgcaacgt tgggtgcgcc
     9421 ggtgtttatc tggttattgt taaaagcagg acgttagccg caaaaagacg gtctatgatt
     9481 aaaagctaag atttttactg accacaccca ggagaaacga tgcaagattc cattctgacg
     9541 accgtagtga aagatatcga cggtgaagtg accacgctgg agaagtacgc cggtaatgtg
     9601 ctgttgattg tcaatgtcgc ctcaaagtgt ggcttaacgc cgcaatatga gcagttggag
     9661 aatattcaga aagcctgggc cgatcgaggt tttgtggtgc tgggattccc gtgcaaccag
     9721 tttctggaac aagaaccggg cagcgatgaa gagattaaaa cttactgtac caccacatgg
     9781 ggggtgacgt tcccgatgtt cagtaagatt gaagttaacg gcgaaggacg ccatccgctg
     9841 tatcaaaaat tgattgccgc agcgccgacc gcagtcgcgc cggaagagag cggattctat
     9901 gcccgtatgg tcagcaaagg tcgtgcaccg ctgtaccctg atgatatttt atggaatttt
     9961 gagaaatttt tagttggcag ggacggattg gtcatccagc gtttttcccc ggatatgacg
    10021 ccggaaaacc ccattgtgat ggaaagcatt aaactggcgt tggcaaaata atgtctattg
    10081 tgatgcagtt acaggatgtt gcggaatcta cccgcctagg gccactttct ggcgaggttc
    10141 gggctgggga gatcctgcac ctggtggggc cgaatggcgc gggtaagagt accttactgg
    10201 cacgaatggc cggaatgacc agcggtaagg gaagtattca gttcgcgggg caaccactgg
    10261 aagcatggtc cgcaacaaaa ctcgcgctgc atcgcgccta tctttcacaa cagcagacgc
    10321 cgccgtttgc aatgccggtc tggcactatc tgacactgca tcagcacgat aaaacgcgta
    10381 ccgaactact gaatgatgtc gcaggggcgc tggctcttga tgacaaactc ggacgtagca
    10441 ccaatcaact ttccggcggt gaatggcaac gcgtacgtct tgctgcggtg gtgctgcaaa
    10501 tcacaccaca agccaatccc gcaggccaat tgctgcttct tgatgagccg atgaacagtc
    10561 ttgatgttgc gcaacaaagt gcgttagaca aaattctgag cgctctgtgt cagcaaggac
    10621 tggcgattgt gatgagcagt cacgatctca accacacatt gcgtcatgcg catcgggcgt
    10681 ggttgctaaa aggtggaaaa atgctggcca gtggacgcag agaagaggtg ctcacgccgc
    10741 caaatctggc gcaggcctat gggatgaatt ttcgccgtct ggatatcgaa ggtcacagaa
    10801 tgctgatttc gaccatctga tttcattagt atcttgaaaa tcgggtaatt acgacgctaa
    10861 attaatgcaa gaataaaaaa cagaggaacc gtacggaatg cgtttctgcc ttattttgat
    10921 cacagcactg tttctggccg ggtgtagcca ccataaagca ccgccgccaa atgccagact
    10981 ttctgattcg attaccgtta ttgccggttt gaacgaccag ctacaaagct ggcatggcac
    11041 gccgtatcgt tatggtggca tgacgcggcg cggtgtggac tgttcgggat ttgtggttgt
    11101 aacgatgcgc gatcgtttcg atttgcagct gccccgagaa accaaagaac aagcctctat
    11161 cggcacgcaa attgataaag acgagttgct gcctggtgac ctggtctttt tcaaaacggg
    11221 ttccggacaa aatggtttgc atgtaggtat ttacgatacc aacaaccaat ttatccacgc
    11281 ctctaccagc aagggagtga tgcgttcctc acttgataat gtctattggc agaaaaattt
    11341 ctggcaggcg agacg
//
LOCUS       DAEEKQ010000125        11323 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.126, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000125 DAEEKQ010000000
VERSION     DAEEKQ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.126"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(239..802)
                     /locus_tag="KLK57_003095"
     CDS             complement(239..802)
                     /locus_tag="KLK57_003095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE0893400.1"
                     /translation="MHSQLRERIRLMRARLDNAAPVAEIRAESQLFVSPAPICDRLVT
                     LAEISNRDHILEPSAGTGAILRAIRDTAPEAMCDAVEINSGLVRYLRENFNGVRVQCG
                     DFMEWQSVQYYSRIIMNPPFSHGQDIRHILRAFSLLRPGGVLVAVCLNGPRQQEKLLP
                     FSDVREELPRGTFAYTDVPTMIIRLRA"
     gene            complement(849..2210)
                     /locus_tag="KLK57_003096"
     CDS             complement(849..2210)
                     /locus_tag="KLK57_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3560 domain-containing protein"
                     /protein_id="HBE0893401.1"
                     /translation="MTLAAITMTAPEAASPVQMYRATYSPDDNKLRLYAASRLDPETY
                     KKVHDAGFRWAPRQALFVAPAWTPGREDVLLSLAGEIEDEDSTLAERQEARAERFTGY
                     SGKRASESAQALDEVERLAAMIPPGQPILVGHHSERRARRDAQRIENGMKRAVMLFER
                     AEYWEERARSALLHAKYKERPDVRWRRIKKIEADLRKAEKTIAQSQKYLTMWRAESLD
                     LNMAKLISSHDHISACFPLDTYPRPAEKSQYEGSRSLWSALDDDIITTEQAREIAIRC
                     HERQIQHQQRWVNHYQNRLIYERAMLDESGGVVTRTQDFEPGGQVFSRGEWLTIIRVN
                     KSNGAVSSVTTPNYSFLGYSGTMKVTPDRITDYKAPSAEEAAVASQAAKRPPVVNYPG
                     EGFREMTKAQWAALPRDCKAVRSVEEAEDHGAYRYRRTMDNNFRLVNVYITDMKITEI
                     PQK"
     gene            complement(2262..2492)
                     /locus_tag="KLK57_003097"
     CDS             complement(2262..2492)
                     /locus_tag="KLK57_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893402.1"
                     /translation="MTVSIVSPSAAAVKPRRHPRFRREDIPAPEIDPVLKAFGRHIAR
                     SFHRGRGVHIPAMKNTAFGQVLRTLELKRAFN"
     gene            complement(3530..3721)
                     /locus_tag="KLK57_003098"
     CDS             complement(3530..3721)
                     /locus_tag="KLK57_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893403.1"
                     /translation="MNISTETREILRNYKAVINARRREMGQKPLTTAQIVDEICDFVA
                     NQQAVFLGGHYILQGSINR"
     gene            complement(3718..4140)
                     /locus_tag="KLK57_003099"
     CDS             complement(3718..4140)
                     /locus_tag="KLK57_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
                     /protein_id="HBE0893404.1"
                     /translation="MYCTVKEIIREVLNTDVPDSECVFAVVLTRGDVRHIAQDWSLTD
                     DELETVMQRLDDAFAHGADVSIVHDVVRELMEEKRASRQVTVPAVMLEKVMALAGSEM
                     KRLYAVGSENGGDGDAFVREEREAMDVVLQALDGEHMS"
     gene            complement(4187..4612)
                     /locus_tag="KLK57_003100"
     CDS             complement(4187..4612)
                     /locus_tag="KLK57_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001198918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HBE0893405.1"
                     /translation="MQYAKPVTLNVEECDRLSFLPYLFGNDFLYAEAYVYALAQKMMP
                     EYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSADAAGIILTSLVINRQLW
                     LYHDSGDAGLTQLYRMRDAQLWRHIEFHPECNAIYAALD"
     gene            complement(5030..5806)
                     /locus_tag="KLK57_003101"
     CDS             complement(5030..5806)
                     /locus_tag="KLK57_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893406.1"
                     /translation="MNENSTLNALICRHARNLLLAQGWPEETDVDQRNPKYPGWISIY
                     VLLDAPRLATLLINRHGGVLPPLLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQV
                     SFPYAGEWLTEDEIRAVLAAVRDAVRSVSCRVAEDARRIRAALTTTGQTLLTRQTRRF
                     RLVVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDV
                     LRIPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(5857..6291)
                     /locus_tag="KLK57_003102"
     CDS             complement(5857..6291)
                     /locus_tag="KLK57_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HBE0893407.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMEISLT
                     GHEIRTVLARLEDIPEDQRIESGISSAAVMEIIRNESENRLVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQEVVNQARTLLKNNTHEND"
     gene            complement(6305..6526)
                     /locus_tag="KLK57_003103"
     CDS             complement(6305..6526)
                     /locus_tag="KLK57_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893408.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELEACFKD"
     gene            complement(6527..7210)
                     /locus_tag="KLK57_003104"
     CDS             complement(6527..7210)
                     /locus_tag="KLK57_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBE0893409.1"
                     /translation="MSRFILGDCVRVMATFPGNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(7287..7580)
                     /locus_tag="KLK57_003105"
     CDS             complement(7287..7580)
                     /locus_tag="KLK57_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002430945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893410.1"
                     /translation="MFLGFVWRVTKDVAGLLFLLRRLTRRGRNAACGQCPASGRARDG
                     RFYRSRLALVTVYASPSPFSDERPSSRFRGISSPSKRRRLRYSTVGLTRYRTR"
     gene            7727..8689
                     /gene="parM/stbA"
                     /locus_tag="KLK57_003106"
     CDS             7727..8689
                     /gene="parM/stbA"
                     /locus_tag="KLK57_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HBE0893411.1"
                     /translation="MLVFIDDGSTNIKLQWQESDGTIKQHISPNSFKREWAVSFGDKK
                     VFNYTLNGEQYSFDPISPDAVVTTNIAWQYSDVNVVAVHHALLTSGLPVSEVDIVCTL
                     PLTEYYDRNNQPNTENIERKKANFRKKITLNGGDTFTIKDVKVMPESIPAGYEVLQEL
                     DELDSLLIIDLGGTTLDISQVMGKLSGISKIYGDSSLGVSLVTSAVKDALSLARTKGS
                     SYLADDIIIHRKDNNYLKQRINDENKISIVTEAMNEALRKLEQRVLNTLNEFSGYTHV
                     MVIGGGAELICDAVKKHTQIRDERFFKTNNSQYDLVNGMYLIGN"
     gene            8692..9042
                     /locus_tag="KLK57_003107"
     CDS             8692..9042
                     /locus_tag="KLK57_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001630992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HBE0893412.1"
                     /translation="MDKRRTIAFKLNPDVNQTDKIVCDTLDSIPQGERSRLNRAALTA
                     GLALYRQDPRTPFLLCELLTKETTFSDIVNILRSLFPKEMADFNSSIVTQSSSQQEQK
                     SDEETKKNAMKLIN"
     gene            complement(9165..9446)
                     /locus_tag="KLK57_003108"
     CDS             complement(9165..9446)
                     /locus_tag="KLK57_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893413.1"
                     /translation="MNYDEITKITAERISDYMTEAVNTDSKSVAEMFHNAAWGVLSLW
                     FELVTKIDLDIHKKNRYASYDFRRKIEMQHEEFQKMTEREQVPLLKLPE"
     gene            complement(9574..9808)
                     /locus_tag="KLK57_003109"
                     /pseudo
     CDS             complement(9574..9808)
                     /locus_tag="KLK57_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421253.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(11257..>11323)
                     /locus_tag="KLK57_003110"
     CDS             complement(11257..>11323)
                     /locus_tag="KLK57_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0893414.1"
                     /translation="SKSVELHDKVIGHYLNIKHYQ"
BASE COUNT         2751 a         3056 c         2808 g         2708 t
ORIGIN      
        1 agaaaaaaga agaacagtag cagtagcagt agcagtagca gtagcagtag cagtagcagg
       61 aacaacaaca tcatcagtaa cagacaaatg acaggtctga aaagacagaa gcccccatca
      121 gaacgtccgc gtaatgaaag gggcggcgcg tgagcgcggc ccctttcagc gccaccggcg
      181 cgcggttttt tcccggcgcg gacagacggg catttatcct cccgccagcc ctgtgtcatc
      241 aggcacgcaa acgaataatc atcgtcggga catcggtata agcaaacgtg ccgcgcggca
      301 gctcctcgcg gacgtcagaa aacggtaaca gcttctcctg ctggcgtggc ccgttgagac
      361 agacggcaac cagaacgccc cccggacgca acagggaaaa agcccgcagg atatgccgga
      421 tatcctgccc gtgactgaac ggcggattca taatgatccg gctgtaatac tgcaccgact
      481 gccattccat gaagtcgcca cactgtaccc tgacaccgtt aaaattttcc cgcagatacc
      541 ggaccagccc gctgttaatc tccaccgcat cacacatggc ctccggcgcg gtatcccgaa
      601 tcgcccgcag tatggccccg gtgccggcag agggttccag aatgtgatca cggttgctga
      661 tttccgccag cgtcaccagg cgatcacaga tcggggcagg actcacaaaa agctgagatt
      721 cagcccggat ttcagcaacc ggcgcggcgt tatccagcct tgcgcgcatc agccggatac
      781 gttctcttaa ctgactgtgc atatcattat ctccgtcatc ccccggggat ttcccggggg
      841 gatatacctt atttctgtgg gatttccgtg attttcatgt cggtgatata cacattcacc
      901 agacggaaat tattgtccat tgtgcggcgg tagcggtatg ccccgtggtc ttctgcttct
      961 tccacactgc gcacggcctt acagtcccgg ggcagggcgg cccactgcgc ctttgtcatt
     1021 tcccggaaac cttcccccgg atagttgact accggcggac gcttcgcggc ctgactggcg
     1081 acggcagcct cttctgccga tggtgctttg tagtccgtga tgcgatcggg cgtcactttc
     1141 atcgtgccgc tgtacccgag aaaactgtaa tttggcgttg tgactgaact caccgccccg
     1201 ttgcttttgt tcacgcggat gatagtcagc cactcgcccc ggctgaaaac ctgtccgccc
     1261 ggctcaaaat cctgtgtccg ggtaaccacg ccgccgcttt cgtccagcat ggcacgctca
     1321 tagatcaggc ggttctgata gtggttaacc cagcgttgct gatgctgaat ctgccgttca
     1381 tgacagcgga tcgcaatttc gcgggcctgc tccgtggtga tgatgtcatc atccagggcc
     1441 gaccataacg accggctccc ttcatactgg cttttttctg ccgggcgcgg atacgtatcc
     1501 agcgggaaac aggcgctgat atggtcatga ctgctgatga gttttgccat attcagatcc
     1561 agcgattcag cccgccacat cgtcagatat ttctgcgact gtgcgatggt cttttcagcc
     1621 ttgcgcaaat cagcttcgat ttttttgata cgacgccagc gaacgtccgg gcgttcttta
     1681 tacttcgcgt gaagcagtgc cgaccgcgcc cgttcttccc agtattccgc acgttcaaag
     1741 agcatcacgg cacgtttcat gccgttttca atacgctgcg catcacgacg ggcgcggcgt
     1801 tcgctgtgat gccccacaag aatgggctga ccgggcggga tcatcgcggc cagtctttcc
     1861 acttcatcaa gtgcctgtgc ggattcactg gcccgctttc cgctgtatcc ggtaaaccgc
     1921 tccgcccgtg cttcctgacg ttcagcgagc gtgctgtctt catcctcaat ctctccggca
     1981 agtgagagga gcacgtcttc ccggcccggt gtccaggctg gcgcgacaaa cagcgcctgt
     2041 ctgggtgccc agcggaaacc ggcatcatgc acttttttat acgtctccgg gtcaagacgt
     2101 gacgcggcat acaggcgcag tttgttgtca tccggtgagt aggtcgcgcg gtacatctgc
     2161 acagggctgg cggcttccgg ggcggtcatg gtgattgcag ctaatgtcat aaaatgttct
     2221 ccttccgtct gttgccgccc ctcgcggggc ggctcacctc atcagttaaa agcgcgtttc
     2281 agttcgagtg tgcggagcac ctgcccgaaa gccgtatttt tcatggcggg gatgtgtaca
     2341 ccacgtcccc ggtgaaaact ccgggcaatg tgtcgcccga aggctttcag cacggggtca
     2401 atttccggcg cggggatgtc ttcgcgtctg aaacggggat gacggcgggg ctttacagcc
     2461 gctgcggaag gtgaaacaat acttaccgtc ataaatgaaa ctccttatga ctgttgaatg
     2521 gaaccgcaga aactgctcga acagtttttg cggtgtggat taagcaggtc ctccccggcc
     2581 tgcttttttc atatctgcgt ctgacgttct ccggtgtttt cctccttctg ttcctgcgtt
     2641 ctcattacct gccagccgga tgactgacag aacaaataaa tgcgtattgc cggagcctga
     2701 aataaatcct gatttttaaa gaacatcacc cgttgagttt cgggagccgc ggttttgacg
     2761 tttcgcggga acctgctgct gtattccctg tggtatgaac ttcacccgtt tgagaacctt
     2821 cgccgcaagg ggcatcactg ccacggcctg acgcacctgt cctgggcaga acgcaaaatt
     2881 ttttaccggt ttttgggtaa aaaattttgt gggctgttca tttaccctgg acagtcagtg
     2941 cggcaggttg tggctgtcta tgcccccggc gacagggact catacgggtg tgtggcaccc
     3001 ggcaggcaat gagcaggggc ccgccgcaac gacggagagc gcgtgcgcga aacggcgttg
     3061 ccccttacat cccggcggcc ttttgccggg atgccgggag cgcaggccac ggcgcgacag
     3121 aggcggcgcg accggaacgg tcgctgcaag ccaggggttt tcacgcatcg gaccgtagcg
     3181 gcagcccgaa accgcgccag cgggttcggc ggagactggc aggtgcgcca gcagctgcct
     3241 gtcggagtcg ggcctgacga ccggatgccg ccaggcagcc gggagccgaa ggtgcgcccg
     3301 gcagacagaa caacactgca gtagtcagaa tcagagcagt agataaagga acaacaacat
     3361 caggaacagt tccataacat aagaaagaaa cactcctgag tcagacacat cacagacggg
     3421 aagggtaacg ggcgacgcgc cagcgtggcc cgcttccctt ccggcaggcg tcagcctgcc
     3481 aggggttttc gcgcgggctg acaggcggcc tccaccgcct gcccctgtat cacctgttta
     3541 tgctgccctg aaggatgtag tgaccgccga ggaatacagc ctgctgattc gccacaaaat
     3601 cgcagatttc atcaacaatc tgcgcagtgg tgagcggttt ctgccccatt tcacgacgcc
     3661 gcgcattaat cacggctttg taattgcgca gaatttcgcg ggtttctgtg ctgatattca
     3721 tgacatgtgc tccccgtcca gcgcctgtaa cacaacgtcc attgcttcgc gttcttccct
     3781 gacgaatgcg tcaccgtcgc ccccgttctc gctcccgacg gcatacaggc gcttcatttc
     3841 actgcctgcc agcgccatca ctttttccag catcaccgcc ggaactgtca cctgacggct
     3901 ggcgcgcttt tcttccatca gctcacgaac aacgtcgtga acaatgctga catccgcacc
     3961 atgcgcaaag gcatcgtcca gtcgctgcat gacggtttcc agctcatcgt ctgtcagact
     4021 ccagtcctgg gctatgtgac gcacatcccc acgggtcagc accacggcaa aaacgcattc
     4081 actgtccggc acgtctgtgt tcagcacttc gcggataatt tctttaacag tacagtacat
     4141 gttctcgtct cctttacagg ggcggcattg ccgccccgtc cgttaatcag tccagtgccg
     4201 cgtaaatcgc gttgcattcg gggtgaaatt cgatgtgacg ccacaactgc gcatcgcgca
     4261 tccggtaaag ctgggtcagt cctgcatccc cgctgtcgtg gtacagccac aactggcggt
     4321 taatcacaag ggaagtcaga atgatgcctg cggcatcagc actcacggta cggtcaaacc
     4381 agtttgcacc gttcaccatg tggaaacggt cgccatccgg catcatgtaa ccgccaccgt
     4441 ccggcaggcg gatgaagtgc cagaagcctc cctgatattc cggcatcatt ttttgcgcca
     4501 gcgcgtacac atacgcctcg gcatacagaa aatcgttgcc aaacagatac ggtagaaagg
     4561 agagacggtc gcactcttca acgttcagag tgacaggttt cgcatattgc atgatgtttt
     4621 cctccgttgt tcacatattc cgcgacaccg ggcggtgccg tgcctccata aaagccgggc
     4681 gataaggcgg ggatgcacag ggcgcaaggg aagccgcagc ctgacgcaca cggctggcgg
     4741 gagttggagg gtgagaccgc gaaatctgag cgggtgaacg accggaaaat cacggcagac
     4801 gggaaggagg gaccgggtga ccgcagcggc gaagcgtacc cttgcatccc tgccagcccg
     4861 gctacgatgg aggcacggca cagcccgcac acgacgctga ggccacataa ctgaaaaccc
     4921 acaaggccat gccccgcatg gccgggtaaa gcggagccgg cggcgacttc ctttgtgctc
     4981 tgtccgggga ccggcaggaa aaaggcgggg atttaccccg ccaacgtcat catttccgga
     5041 tttttgccag ggcatccgcc atgctgcgga tttcggcccg ggcttcccgg accacctccc
     5101 gcagaacacc acggtcaaac cagcggcggg gcggttcatc cggtatacgc agcacatcgc
     5161 aggccagccc actggacagg atatgctcaa tacaatcgct gaccaggtac atttccaccg
     5221 acgaaaaacg tgctccccgg ttcacgatgg catcgagcac cacgggcagg ttttcgtcat
     5281 cttcatcgag ccagcagggg tgatcgcttt ccttcacaac cagacgaaag cggcgcgtct
     5341 ggcgagtcag caacgtctga ccggtggtgg tcagcgccgc ccggatacgc cgcgcatctt
     5401 ctgccacccg gcaactgaca ctgcgtaccg catcgcgcac cgccgcaagt acagccctga
     5461 tttcgtcttc ggtcagccat tctccggcat acgggaaaga cacctgcgtt ccgtctgccg
     5521 gaagtaccgg cagcgactgc cactgactgc cggacagtac cagttccgcc ccggttccgg
     5581 tcagtttctg aatggcggag gccaggagcg gcggcagtac gccgccgtga cggttgataa
     5641 gtaacgtcgc cagccggggc gcatccagca gaacataaat gctgatccag cccggatact
     5701 tcgggttacg ctggtcaaca tccgtctctt ccggccagcc ctgcgccagc agcaggttgc
     5761 gggcgtgacg acaaatcagt gcgttcagcg tgctgttttc gttcatgatt aagcgttctc
     5821 cctgtatcgc cgcccgcagg gcaggcggcg ataacatcag tcgttttcgt gtgtgttgtt
     5881 tttcagcagg gtgcgggcct gattaaccac ctcctgacgg cgggtgacgc attccgggac
     5941 ggcgaggcca tgatcccggg cggcccattc acgtttccac aatgcctgtt cagcggtctg
     6001 aatcagggac gccagcagtt cagcagggac ggtcaccagg cggttttcgc tctcgttgcg
     6061 gataatctcc atcaccgcag cggaagaaat accggactca atccgctggt cttccgggat
     6121 gtcctccagg cgtgccagga cggtcctgat ttcatgaccg gtcagagaaa tttccatacc
     6181 gtcagccagt gcctgaatct cttccgggga ccagataacc agcatcagcg gcatgtctcc
     6241 cggatacttt actgccagct cccggcatag cgtttcgcat gttccgtaca ttctcgtctc
     6301 cttctcaatc tttaaagcac gcttccagtt ccagaatttc ggtatatgcc tccatcagga
     6361 ggcggtttgc gcgaaacagc gtctgacgca ccagacgttc cggcagggtg tcggcattaa
     6421 agctgtaccg ctgctccatc tcattcatgg ttgtacgcag gtcgcacatg gcattggcca
     6481 cctccgccac ttcggcgcgc agtttttcgt gtcctgcata gttcatttac gcagcctccg
     6541 gcataaacca gtcatcattc gcggccccct gctgcatggc ccgttgcacg gcggcaaggc
     6601 gttgctgccc ggcacggtga tactgctcaa gcagctcgat accgatatac cggcgtccgg
     6661 actggagggc ggcgacgcag gttgagccgc tgcctgcaaa cgggtccagc acaattgcgt
     6721 tcgggtgtgt gaagctctca atcagcggtt gcaggctggt aacgggcttt tccgtcgggt
     6781 gatggcgatt gcccgaatat ttccagccca gcacgtccgg cagcgggttt tgtggcagac
     6841 gtggacggcc ttttgccagg atgtaggcac attcgtggcg atagcccaca tatgcggcct
     6901 tcgatgtgta gtttttggtg aagaccaggt gaccaacaac gctgaatccc gcatttttcc
     6961 aggcggccat aaagcgatcg acgcggttcc agccatagaa gctcaccatt aacgcgtctt
     7021 ttttgagtac gcggtacatt tcattacagg ctggttgcag ccattcatcg gttttatcgc
     7081 cggcgatggt gcgcccggaa cggtcacgga aaccgacgag atacggcggg tcggtgagga
     7141 tgaaatcgac agcattgccc ggaaacgtgg ccataacacg cacgcagtca cccaggataa
     7201 aacgggacat aacagcactc cttctcttac agagggcacc gggagggcac ccctgtggtg
     7261 cccgttcccc gctgcctgtg ggctgttcac cgcgtccggt aacgggtcag gccgaccgtg
     7321 gaataccgca gccgtcgtct ttttgacggc gaggatatgc cgcgaaagcg gcttgaaggg
     7381 cgttcatcag aaaagggcga cgggctggcg tacactgtca ccaacgccag ccgggagcgg
     7441 taaaagcgcc cgtcccgtgc ccggccggac gcgggacact gaccacaggc agcgttcctg
     7501 ccgcgccgcg tcagccggcg cagtaaaaag aggagcccgg cgacatcttt tgttacccgc
     7561 caaacaaaac ccaaaaacaa cccataccca acccaataaa acaccaaaac aagacaaata
     7621 atcattgatt gatggttgaa atggggtaaa cttgacaaac aaacccactt aaaacccaaa
     7681 acatacccaa acacacacca aaaaaacacc ataaggagtt ttataaatgt tggtattcat
     7741 tgatgacggt tcaacaaaca tcaaactaca gtggcaggaa agcgacggaa caattaaaca
     7801 gcacattagc ccgaacagct tcaaacgcga gtgggcagtc tcttttggtg ataaaaaggt
     7861 ctttaactac acactgaacg gcgaacagta ttcatttgat ccaatcagcc cggatgctgt
     7921 agtcacaacc aatatcgcat ggcaatacag cgacgttaat gtcgttgcag tgcatcacgc
     7981 cttactgacc agtggtctgc cggtaagcga agtggatatt gtttgcacac ttcctctgac
     8041 agagtattac gacagaaata accaacccaa tacggaaaat attgagcgta agaaagcaaa
     8101 cttccggaaa aaaattacat taaatggcgg ggatacattc acaataaaag atgtaaaagt
     8161 catgcctgaa tctataccgg caggttatga agttctacaa gaactggatg agttagattc
     8221 tttattaatt atagatctcg ggggcaccac attagatatt tctcaggtaa tggggaaatt
     8281 atcggggatc agtaaaatat acggagactc atctcttggt gtctctctgg ttacatctgc
     8341 agtaaaagat gccctttctc ttgcgagaac aaaaggaagt agctatcttg ctgacgatat
     8401 aatcattcac agaaaagata ataactatct gaagcaacga attaatgatg agaacaaaat
     8461 atcaatagtc accgaagcaa tgaatgaagc acttcgtaaa cttgagcaac gtgtattaaa
     8521 tacgctcaat gaattttctg gttatactca tgttatggtt ataggcggtg gcgcagaatt
     8581 aatatgcgat gcagtaaaaa aacacacaca gattcgtgat gaacgttttt tcaaaaccaa
     8641 taactctcaa tatgatttag ttaacggtat gtatctcata ggtaattaat gatggacaag
     8701 cgcagaacca ttgccttcaa actaaatcca gatgtaaatc aaacagataa aattgtttgt
     8761 gatacactgg acagtatccc gcaaggggaa cgaagccgcc ttaaccgggc cgcactgacg
     8821 gcaggtctgg ccttatacag acaagatccc cggacccctt tccttttatg tgagctgctg
     8881 acgaaagaaa ccacattttc agatatcgtg aatatattga gatcgctatt tccaaaagag
     8941 atggccgatt ttaattcttc aatagtcact caatcctctt cacaacaaga gcaaaaaagt
     9001 gatgaagaga ccaaaaaaaa tgcgatgaag ctaataaatt aattcaatta ttattgagtt
     9061 ccctttatcc actatcaggc tggataaagg gaactcaatc aagttatttt cttaccagtc
     9121 attacataat cgttattatg aaataatcgt ttgcactgtc tctgttattc aggcaatttc
     9181 aataaaggca cttgctcacg ctctgtcatt ttctgaaact cttcatgctg catttcgatt
     9241 tttcttctaa aatcataact tgcatacctg ttctttttat gtatatccag atctattttt
     9301 gtgacgagtt caaaccacaa tgatagcact ccccatgcag cgttatgaaa catttccgca
     9361 acagatttag agtctgtatt aaccgcttca gtcatgtagt cgctaatacg ttctgcagtg
     9421 attttcgtta tttcatcgta attcatttta tcccctacaa gactatttaa tggaatgaaa
     9481 aatatttggt ctgataacaa tgctcctgaa ttaacacctg caggctatgt taaagttgcc
     9541 ctcacacaca atctacctgt tttctcgtgt gtgtcacagg cgcaatacat aaatagtctg
     9601 gccattaagt tcgtaacgga tttcgtactc gccagcaaaa atccgtctga catccctggt
     9661 ttcaaactga aacaactgtt catcccatac gtggatgagt taacagaatg accggcgcct
     9721 gtgtcaggga ctacaccgtt ttggctgcag ctggtttact gaccaacgcc agaaaatcat
     9781 ataactgcgt caatgtcagc gtatccagcg cgactgtatt atttttctct gcaaccagaa
     9841 tattgcatta ggttattacc aggtgttcta atgtacaggt aaagacgttc tttataatct
     9901 gaaatctcta cttttaatcc ctcagacttt gcctgctgaa agccctcgtc aaggtgcgct
     9961 tttattaggt taatgtctgg atgtcttgta tcgggggcaa ttctgtattg gtgtaacgat
    10021 ggggtggatg ttccctgcaa taagacctcc agatagagat tggcacaacc attcccatta
    10081 tttctaatag tttattggta atcacaaact gtatatttag ccattatatt gaatcctgtg
    10141 aagtaaacgg cgacggctca aaattttatc aaacctaaga taattctacc atcaaagtta
    10201 ataatttgaa tttatatgac ggctctatgg gggggtaaaa atccccgtgc agagaggcga
    10261 ttttcagagt tgaaaacgtc gtcgttaaga acaccaaagt cagtaccacc aacccggaaa
    10321 gtagcttcat gcaccgggac aacagcagtt ttggtgtccg aaatcgtaca attctgtaca
    10381 tctacgcaag gaaatgtacg gaagtaagcc aaatggttat cggacggtcg gaacatttcg
    10441 tacagccttt acgttcagtg agttagctgg cctagatgta accactcgtt caactcaatt
    10501 ctcttagcgt gggaaaattt ccgttttcca agcggccccc tcaagcggac cccttatagt
    10561 acaataattt tcgatatcca aactgccccc aattacatct ccagtctctg gcagcagaga
    10621 tcatatattt ccattgccgc cgtagccacg atattctggc tattcacaaa aacaatttag
    10681 ccccaaagcg cagtaaaaaa gtacactatg aaggtgtatg ataccttcat agtgcttaag
    10741 taattatgag taatataatc tgtgtaaact actaattgct tcaccgtcag tgattaaatg
    10801 cgtagctgtt ccttgtctta tataatacct attttcaaaa tgacagactt ctccgacact
    10861 ttttatttcg atttttaaaa catgcagtcc ttcatatgaa cataatgaaa tatcttttag
    10921 tatttgttgt ttcaaagcct cattaaaatt aaatgattct attttttgtt ttatgaacag
    10981 aaagtaatta tctatgtttt ttgattgaat tacagcttca tgatcaattc cattgatata
    11041 atagccattg cactcaatag gggtaacacc atataaagtc ttaactctgt ctgccgtagc
    11101 tttggtatcg gaaacaccaa ctaaaatata accattagat tctctaccga tgttatttat
    11161 ggcgacagcc gtttggacta tattaatgaa acattcttcg tctattttat tctcgcctga
    11221 gagaataaat aatccctgtt tggtaatgac tccaacttat tgatagtgtt ttatgttcag
    11281 ataatgcccg atgactttgt catgcagctc caccgatttt gag
//
LOCUS       DAEEKQ010000126        11266 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.127, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000126 DAEEKQ010000000
VERSION     DAEEKQ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11266
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.127"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..210
                     /locus_tag="KLK57_003111"
     CDS             <1..210
                     /locus_tag="KLK57_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0893415.1"
                     /translation="KIVPLTVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMF
                     AQTTKGVLETGIYRGVVSIYLSQDI"
     gene            302..2986
                     /locus_tag="KLK57_003112"
     CDS             302..2986
                     /locus_tag="KLK57_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HBE0893416.1"
                     /translation="MDKKLLALLILASLSPAEATLTEIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLAHHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSCYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            2983..4071
                     /locus_tag="KLK57_003113"
     CDS             2983..4071
                     /locus_tag="KLK57_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HBE0893417.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGDFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(4154..5451)
                     /gene="exuT"
                     /locus_tag="KLK57_003114"
                     /pseudo
     CDS             complement(4154..5451)
                     /gene="exuT"
                     /locus_tag="KLK57_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
     gene            5934..7346
                     /gene="uxaC"
                     /locus_tag="KLK57_003115"
     CDS             5934..7346
                     /gene="uxaC"
                     /locus_tag="KLK57_003115"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HBE0893418.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            7361..8848
                     /gene="uxaA"
                     /locus_tag="KLK57_003116"
     CDS             7361..8848
                     /gene="uxaA"
                     /locus_tag="KLK57_003116"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HBE0893419.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINIRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8931..9482
                     /locus_tag="KLK57_003117"
     CDS             8931..9482
                     /locus_tag="KLK57_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HBE0893420.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(9487..10731)
                     /gene="sstT"
                     /locus_tag="KLK57_003118"
     CDS             complement(9487..10731)
                     /gene="sstT"
                     /locus_tag="KLK57_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HBE0893421.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         2961 a         2754 c         2924 g         2627 t
ORIGIN      
        1 aagattgttc cgctgacagt aacctggggc ggtgaagaaa ttaaagctga tgcggcaacc
       61 acttttaccg caaccaaaat ctttgcttcc gatgcgttaa ctaacggttc tctggctaaa
      121 aacctgatgt ttgctcaaac caccaaaggt gttctggaga caggcatcta ccgtggtgta
      181 gtaagcattt atctgtctca ggatatctaa tccagataac agaattaaca cgtaacaaag
      241 ggaggggtag ccctccctta ttttaactgt tttagtattt gttatggatt tcaggattgc
      301 catggataaa aaattactgg ctcttttgat cctggcgagt ctcagcccgg cagaggcgac
      361 attaaccgaa attcccgcag ggtttgaggt tattgctcag ggacagcagg agtatatcga
      421 ggtttatttt tcagggaaaa gtctcggtaa atattatgca atggttaatc ttgataccgt
      481 aacatttctt gatccagcaa gtctatataa caagctggaa ctggatgtag acgatcagaa
      541 aatcgcgcat atagtgaaag aaaaattatc gcagccgcta gctcaccacg gtgaattggc
      601 ttgcggttat gtacgtactg actcagggtg tggttttctg aataccgata cgctggaaat
      661 aatctataat gatgaagaaa gttcggcaac gttgtttatt aatccgcaat ggaattcagc
      721 tttcgatgcg aagtcattat atttaaatcc agacaaaaat actgttaatg cttttataca
      781 tcagcaagac atcaatgttc tggcacagga tgattaccaa tcgttgtcta ttcagggaaa
      841 cggtgcgctg ggaataacag aaaatagtta tattggtgca cactggaatt tcaacggtta
      901 tgatgcagat gatgtcagtg acagtaatgc tgacgtcagc gatctctatt atcgttatga
      961 ttttttacgt cgttattatg tgcaggcggg tcgcatggac aaccgcacac tatttaatgc
     1021 acaaggcggg aactttacct ttaactttct gccactcggt gcaattgatg ggatgcgtat
     1081 cgggtcgaca ctgagctatt taaaccaggc gcaaagccag caaggaaccc cagtaatggt
     1141 tctgctttcg cgcaattctc gtgttgacgc ttatcgtaat gagcaacttt tgggatcgtt
     1201 ttatctcaat agtggttcgc aatttattga taccagttcc tttccgccag gcagctacag
     1261 cgtggcatta aaagtctacg aaaataacca actcacccgc accgagcttg tgccgtttac
     1321 caaaaccggc ggtctgactg acggaaatgc gcaatggttc ttacaggcag gtaaaactac
     1381 atcacaggtt tctgatgatg aaagttcagc ttatcagcta ggagtacgcc tgccattaca
     1441 tccgcaatat gagctctacg cagggctggc gaatgccgat gatgtgagtg ctttcgagtt
     1501 aggtaataac tggacggcag atttaggcgg ggtggggaat cttgcaatca gcgccagcgt
     1561 gttccgtaac gatgacggcg gcaaaggtga tatgcaacag gccaactgga gtaatccggg
     1621 atggccgacg ttgggctttt atcggaccaa ctctgacggc gatgcttgta caaccgacag
     1681 cagagagagc tataacgcct taagctgtta tgaaagtatt tccgcgacgg tttcacagaa
     1741 ttttgtcggc tggaatatga tgctgggtta tacccgcaca caaaataaca ctgatgatag
     1801 tttgcgttgg gataaacagc agagctttga aaataactat cttcgccaga caacagcgca
     1861 aagtatctct gaaactgtac aacttagcgc ttcccgcgct tttgtgatgc gtgactggat
     1921 tttgagtacg tcagtcggtg ttttccatcg taatgacaac ggtggcgata acgacgacaa
     1981 cggcttgtac ttatcgtttt cgttatctga cacgccaacg atggatagca ataacaacag
     2041 ccattcaaca aatgtgtcta cggattatcg ttatagcgaa caggatggcg atcaaacgtc
     2101 atggcagtta tcgcatacct tttataacga ttcattcagc cataaagaac ttggcgtaac
     2161 cgtcggaggc ctgaacaccg ataccataaa cagcgcggtt aacgggcgtt gggatgggca
     2221 atacggaaat gtctacgcta ccgtatctga tagttatgac cgtaagaatc atgatcatct
     2281 ttcggccttt acgggcactt atagctctac gctggctgtc agttgctatg gcgttaattt
     2341 gggagccagt ggtacagacg atttgctggg tgcggtactg gtggatgtga aaggcttctc
     2401 tgaacaggat gaagagagtc aggatctgca actcgaagcg cgggtggcag gcagccgaac
     2461 tttgcagctt ggtcaaagtg acagtgtgtt gttcccttat cctggatttc agtctggttt
     2521 tgttgaggtt aacgacagta gccagggcaa tcagcagggg acaacaaaca tcattaacgg
     2581 tgcagggaat cgtgaattaa tgttgttgcc tggcaagctg cgctatcgcg aagtgtctgc
     2641 cagctttaac tacaactata tcggtcgttt attactgccg gcagcggtga aaaaattccc
     2701 gattgtgggg ctgaacagcg ccatgttact ggtagctgaa gatggaggat ttacacttga
     2761 aattaacggc agcgaaaaag agctctatct gctttccggg cagcaattcc ttaagtgtcc
     2821 actgagtgtt gtaaagaaac gcgccagcat tcgttacagc ggagatgtta cttgtagtgt
     2881 ggtgacttat tcacaattac cggagtcgat tcaggttcag gcacagttga aacagcctaa
     2941 attacgtgga aacgttcaga cggcgcaaag ggaggttgca ccatgagaaa tcgattgatt
     3001 gcggcgatat tgggcttgtt tggcacgctc actggcgttc aggcagctcc tgacgtgacc
     3061 agtgaaatta cgtatgattt ggcatctggt agagcggatt attacttctg gaaggatgaa
     3121 gcgtcggcag gaaataatgg atatatgtgg tatgaatgtt cgtatcctga cctccaacaa
     3181 acctgtacag ctaatggaaa tatatcgaca gtacaaatct atttaactga acaacgcagt
     3241 gggatgcgtt ggccggtaaa actcaaagga tttaaaacag ccattgtaag tagcgatgaa
     3301 gcgccgccag gatgcaaggg gggcaaaggg cttcagacga atcttaagga ttctaataga
     3361 tcttcatgta cagaagatgg tcaacattat tatatatacg atacaaagtt tcttacgctc
     3421 taccttgagc agacagagat gaagaatttg ccgattggtg gcgtctggaa ggggaaagtt
     3481 aaattacatt cgaacagccc ggcccaggac tatttcgcaa atattaccct gaatacgctc
     3541 gaccccaacc atattgacgt gttcttcccg gagttcgccc acgccacgcc aagggtgcag
     3601 ttagacttgc atccaacagg aagcgttaac ggcagcaact acgcgcaaga tctgaccatg
     3661 ttggacatgt gcctgtacga tggttttaac ggtaatgcca tcagttatga aatcatgctc
     3721 aaagatgaag ggcgaccagc tgcagggcgc agagacggtg acttctctat ctatcgtcaa
     3781 ggagggacca ccaccgacga gggagaacgc attgattacc gggtcaaaat gtacaacccg
     3841 gaaaccggtg ggcaaattga tgtgcgcaat aatgaaaata tggtctggaa cagcattaac
     3901 ctgaaacgtg tgcgtccggt ggtactgccc ggtattcgct atgccgtgat gtgtgtgccg
     3961 acacccttga ctctggcagt cgataaattc agcgtgatgg ataaacaggc cgggtactac
     4021 atgggcaaat tgtcagtaat ctttacgcct tccttgccaa ccatcaattg accccgcaga
     4081 ccagaaaaaa ctaaacaacg aagccgcctg ataaggcggc ttcgtcatta caacttacgt
     4141 taaacgttaa acgttaatgt tgcggtgcgg gatcgttatg tgtttcctgc gccacctcaa
     4201 tcgccggttt gttctgcaat acggtccaga taaccagcgc acccagcagg tcaaatactg
     4261 ccagaactgc gaacagcggg ctgaagccga tggtgtcagc cagtgcaccg acaaccagcg
     4321 caaacagcgt acttgccagc catgcggaca tcccggttaa accgttcgcc gtcgccactt
     4381 cgttacgacc gaacacgtca gaagagagcg taatcagcgc gccagacagt gcctggtggg
     4441 caaaaccacc gatacacagc agcataattg cgacatacgg gttggtgaac agaccgatca
     4501 tacccgggcc aatcatcagc acagcaccca gcgttacgac catcttacgg gaaacgatca
     4561 ggttcacacc aaaccaacgc tggaacagcg gcggcaggta accaccgagg atacaaccga
     4621 ggtcagcaaa cagcatcggc atccaggcga acatcgcgat ctctttcagg ttaaagccgt
     4681 aaactttaaa catgaacagc gggatccacg cgttaaaagt accccaggcc ggttctgcca
     4741 ggaaacgcgg cagtgcgata ccccagaact gacggttacg caggatctga ccaacggaca
     4801 ttttcttcgc cgtgctcacc tggtgctggg cttcctgacc attaataata tagtcgcgtt
     4861 cttcatcggt cagatgcttc tggtcgcgcg gatgtttata gaaaatcagc catgccatcg
     4921 cccagataaa gctcaatgca ccggagatga taaatgccat ctgccagctg tgcatcacga
     4981 ttgccatacc accagcggcg gcgcaatcat cgcaccaatc gaagaaccta cgttaaagta
     5041 gcctactgcg atggaacgct ctttcgccgg gaaccattcg gagctggctt tcagacccgc
     5101 cgggatcatc gctgcttccg cggcaccgac cgcaccacga gcaacagcca ggccacccca
     5161 gctacctgcc agcgcggttg caccacagaa cacggcccac agcacagcaa acattgcata
     5221 accgattttc gtacccagca catccagtac ataacctgct accggttgca tgaccgtata
     5281 agcagcagaa taggctgcga tgatatagga atactgttgg gtggagatgt ttaactcttc
     5341 catcagagtt ggcgcagctg ccgccacagt gttacgcgtc aggtaaccaa gcacggtgcc
     5401 gagcgtcacc agtgcgatca tataccaacg taacccttta attttacgca tgtaaaacct
     5461 catcgttttg ttatatccgc aggaagcgcg gccatcatcc cgccagaggc tgaagatgtt
     5521 agttacgacc gggtaaccga aaaaaaaccg gcacgccccg aacgttgcca tgaaagtttc
     5581 gtgctaattc ggcttccgta ccggttaacc cgattcatct aacaaatgaa tgaatcggca
     5641 atttgcattc cgtcgactta tgatttgcga cggcagaaag ataacttgtc atacaacttt
     5701 aaaaggtgag agccatcaca aatgtgcgaa tatttgtagg gacattacct gacgacagcg
     5761 aggccagtac tggcgcggcc tgcagcgaga tttatcactt tgagagtaat ttttttaact
     5821 acgtttattg atctaactca cgaaaatatc ttcggactct ggaaattggt gtgataactt
     5881 tgtcagcatc gcaccataag caagctagct cactcgttga gaggaagacg aaaatgactc
     5941 cgtttatgac tgaagatttc ctgttagata ccgaatttgc ccgccgtctg tatcacgact
     6001 acgcaaaaga ccagccgatt ttcgattacc attgccattt gccgccgcag cagattgcgg
     6061 aagactatcg ttttaaaaac ctgtatgaca tctggctgaa aggcgatcac tacaaatggc
     6121 gcgctatgcg taccaacggt gtggccgagc gtctgtgtac cggtgatgca tctgaccgtg
     6181 aaaaatttga cgcctgggcg gcgactgttc cgcacactat cggcaacccg ttataccact
     6241 ggacgcacct tgaactgcgt cgtccgtttg gtatcactgg caaattgctt tctccgtcaa
     6301 ctgccgatga aatctggaac gaatgtaacg aactgctggc gcaggataac ttctccgcgc
     6361 gcggcatcat gcagcagatg aacgtgaaaa tggtcggcac caccgatgac ccgatcgatt
     6421 ctctggagca tcacgcagag atcgccaaag acggctcttt caccatcaaa gtgctgccga
     6481 gctggcgtcc ggacaaagcc ttcaacatcg aacaggcgac ctttaacgac tacatggcga
     6541 agctgggcga agtttccgat accgacattc gccgctttgc tgacctgcaa actgccttga
     6601 ccaaacgtct ggatcacttc gccgctcacg gctgtaaagt gtctgaccat gcgctggacg
     6661 tggtgatgtt tgctgaagcg aacgaagcgg aactggacag cattctggcg cgccgtctgg
     6721 ctggcgaaac cctgagcgag cacgaagtgg cacagttcaa aactgcggta ctggtgttcc
     6781 ttggtgccga atatgcacgt cgcggctggg tacagcagta ccacattggc gcgctgcgta
     6841 ataacaacct gcgtcagttc aaactgctgg ggccggatgt aggctttgac tccatcaacg
     6901 accgtccgat ggcggaagag ttgtctaagc tgctgagcaa gcagaacgaa gaaaacctgc
     6961 tgccgaaaac cattctctac tgcctgaacc cgcgcgataa cgaagtgctg ggcaccatga
     7021 tcggtaactt ccagggcgaa ggtatgccgg gcaaaatgca gttcggttcc ggctggtggt
     7081 ttaacgatca gaaagacggt atggaacgtc agatgaccca actggcgcag ctcggtctgc
     7141 tgagccgctt tgtcggtatg ctgactgaca gccgtagctt cctgtcatac acccgtcacg
     7201 aatacttccg ccgcattctg tgccagatga tcggtcgctg ggtggaagcg ggcgaagcac
     7261 cggcggacat caacctgctg ggcgagatgg tgaaaaatat ttgctttaac aatgcgcgtg
     7321 actacttcgc cattgaactg aactaaggtc tggggttgat atgcaataca tcaagatcca
     7381 tgcgctggat aacgtcgcgg tcgctttagc agatttggct gaaggcacag aagtcagtgt
     7441 cgataaccag actgttacgc tgcgccagga tgttgctcgt ggacataaat ttgcgttaac
     7501 ggatatcgca aaaggggcca acgtcatcaa atatggcctg ccgattggtt atgcattggc
     7561 ggatattgcg gcgggggaac acgtccacgc ccacaatacg cgcacgaatc tgagcgatct
     7621 ggatcagtat cgctatcaac ctgattttca ggatctgcct gcgcaagcgg cagatcgtga
     7681 agtgcagatc tatcgtcgcg ctaacggcga tgtcggggtg cgtaacgagc tgtggatcct
     7741 gccaaccgtg ggctgtgtca acggcatcgc gcggcaaatc cagaaccgtt tcctgaaaga
     7801 gaccaacaac gccgaaggta ccgacggcgt gttcctcttc agccacacct acggctgctc
     7861 acaactgggc gacgatcaca tcaacatccg caccatgctg caaaacatgg tgcgccaccc
     7921 gaacgcgggc gcagtgctgg tgattggtct gggctgtgaa aacaaccagg ttgccgcatt
     7981 ccgtgaaacg ttgggcgata tcgatcctga acgcgttcat ttcatgatct gccaacagca
     8041 ggatgatgag atcgaagccg ggatcgagca tttgcatcag ctgtataacg tgatgcgcaa
     8101 cgataaacgc gagccaggca aactcagcga actgaagttt ggtctggagt gcggtggttc
     8161 tgacggtctt tctggtatta ctgctaaccc gatgctgggg cgtttctctg actacgtgat
     8221 tgctaacggc ggtactaccg tactgaccga agtgccggag atgtttggcg cagagcagtt
     8281 gctgatggac cattgccgcg acgaagcaac gtttgaaaaa ctggtcacca tggtcaacga
     8341 ctttaaacag tactttattg cccatgatca gccgatctac gaaaacccat caccggggaa
     8401 caaagcgggc ggtatcacca cgctggaaga caaatcactt ggctgtaccc agaaagcggg
     8461 ttccagtgtc gtggttgacg tgctgcgtta cggcgagcgt ctgaaaacgc ctgggctgaa
     8521 cttgttaagt gcgccgggta acgatgccgt agcgaccagc gccctggcgg gtgcgggctg
     8581 ccatatggtg ctgttcagta ctggtcgcgg cacgccatac ggtggatttg tgccgacggt
     8641 gaaaatcgcc accaacagtg aactggcggc gaagaaaaaa cactggatcg actttgacgc
     8701 gggtcagctg atccacggta aagcgatgcc gcagttgctg gaagaattta tcgacaccat
     8761 cgttgagttt gccaacggta agcaaacttg caacgagcgt aacgacttcc gcgaactggc
     8821 gatcttcaaa agcggcgtaa cgctataaag tcgaaatgag ttgttaataa aaaacggcgt
     8881 ttcataatgt gaagcgccgt ttgcttttcc atacctgata aggatttatc atgaccgcgt
     8941 attggctggc ccagggcgtg ggtgtcatcg cctttctgat tggtatcaca acatttttca
     9001 atcgtgacga acgtcgcttc aaaaagcagc tttcggtcta tagcgccgtt attggcgtac
     9061 atttttttct tctgggcacc tatcccgctg gtgccagtgc catccttaat gccattcgta
     9121 cattgattac cttacgcacg cgcagcttat gggtaatggc gatttttatt gtgctgactg
     9181 gcggaattgg cctcgcgaag ttccatcatc ctgtcgaact attgccggtt atcggtacga
     9241 ttgtcagtac ctgggcgctg ttccgctgta aagggctcac catgcgctgc gtgatgtggt
     9301 tttcaacgtg ttgctgggtg attcacaact tctgggcggg gtcgataggc ggcacaatga
     9361 ttgagggtag ttttctgctt atgaatggct tgaatatcat tcgtttctgg cggatgcaga
     9421 aaaggggaat tgatccgttt aaagtagaga aaacccctcc cgccgtagac gaaaggggtt
     9481 aaacaattaa ttacgcaggg cgctatttgc cagacgatcg tcttccgcct ggcaagctgc
     9541 cgcagtgaac agcacgtcag ttgaagagtt cagcgcggtt tcgcaagagt cctgcaatac
     9601 gccgatgata aagccgacgg caaccacctg catggcgata tcgttcgaaa taccgaacat
     9661 attacaggcc agcgggatca gcagcagaga accccccgcc acgccggatg cgccacaggc
     9721 acacagagaa gccaccacgc tcaacagcag cgccgtgggc agatcgaccg ggatacccag
     9781 cgtattaacc gcagccagcg tcaacacggt aatggtgatt gctgcgcccg ccatattgat
     9841 ggtggcaccc agcggaatag aaacggaata ggtatcgcga tccagattca gcttttcaca
     9901 cagtgccata ttcaccggaa tgttcgccgc agagctgcgg gtgaagaagg catacacgcc
     9961 gctttcgcgc aggcacagca gcaccagcgg gaacgggtta cgacgaattt tccaccacac
    10021 cagcaatggg ttaaccacca gcgccaccag taacatacag ccaaccagca cgaccagcag
    10081 ttgcgcgtag ccccacagtg tggagaaacc ggtggttgcc agggtagaag aaaccagacc
    10141 aaaaatacca atcggtgcga agcgaatgac cagtttcacc ataaaggtga cggcattcga
    10201 catatcgttg accaggtttt tggtggtctc gttaccgtga cgcagtgcga aaccgaggcc
    10261 aattgcccac accaggatcc cgatgtagtt acctttcagc agcgcgtcga tggggttgga
    10321 aaccatgctc attaccagcc cgcgcatcac ttctacaatg cctgacggtg gcgaaatatc
    10381 acccgcgcta ctggataagt gcagggtaga agggaaggcg aagctgaaaa cgactgcggc
    10441 cagagcagca gagaaggtac ccagcagata gaggaacaaa atagggcgga tattggtttt
    10501 ctgcccatgc tggtggttag caatagatgc catcaccagc atcaacacca ggatgggggc
    10561 aacggctttc agtgcgccga cgaacaaagt acctaacaga ccaacagctt ccgccgcggg
    10621 ttttgatatc catgccagaa gaatccccag aacaaggccg accaggattt gttttaccag
    10681 gctgccatga gccagacgcc ggaatagccc cggtgaacgt tgcgtagtca tttttcgatc
    10741 ctttcattgt gttatcgcac atccctggtg cgttctgtcg acgcatcttt caggatgtaa
    10801 caaagtgttt gcaaggtcga gtataaggaa agttacagct tcgggaagcg aaaaatgctg
    10861 gattttaaag agaagtcata ttttgaaact taatggttac atttgtttga cataaacaga
    10921 gggaaggact ggctactaag gctgattaga taacatatgg gcaaaactat ttggaatatg
    10981 agaacggttg cgctccttta atgtgctatg ccatataaag tcactggcgc agggagcgcg
    11041 cagggggcgg ccaatcgccg ccgcgcgatt ccctcagctt atcccttgcg gctaccgggt
    11101 cgggcgcaag ggaacgtccc tgtaagatgc gccctcgccg cgcatccatg cgcgtcgccc
    11161 cggcctccag ggaacgcttc ggcgattttg acgccaccaa cacccgtgcg gtctttttgt
    11221 aaagagctac aagccgatta cataattaat agctcacctt tgtctg
//
LOCUS       DAEEKQ010000127        11242 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.128, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000127 DAEEKQ010000000
VERSION     DAEEKQ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11242
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.128"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..308
                     /locus_tag="KLK57_003119"
     CDS             <1..308
                     /locus_tag="KLK57_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HBE0893422.1"
                     /translation="VLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFF
                     DQQIRWAKADLVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            507..788
                     /gene="ppiC"
                     /locus_tag="KLK57_003120"
     CDS             507..788
                     /gene="ppiC"
                     /locus_tag="KLK57_003120"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HBE0893423.1"
                     /translation="MAKTAAALHILVKEEKLALDLLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="KLK57_003121"
     CDS             complement(875..2350)
                     /gene="ilvC"
                     /locus_tag="KLK57_003121"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HBE0893424.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRCHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            2500..3393
                     /gene="ilvY"
                     /locus_tag="KLK57_003122"
     CDS             2500..3393
                     /gene="ilvY"
                     /locus_tag="KLK57_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HBE0893425.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="KLK57_003123"
     CDS             complement(3445..4989)
                     /gene="ilvA"
                     /locus_tag="KLK57_003123"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HBE0893426.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="KLK57_003124"
     CDS             complement(4992..6842)
                     /gene="ilvD"
                     /locus_tag="KLK57_003124"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HBE0893427.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSEQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="KLK57_003125"
     CDS             complement(6907..7836)
                     /gene="ilvE"
                     /locus_tag="KLK57_003125"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HBE0893428.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKCIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="KLK57_003126"
     CDS             complement(7856..8119)
                     /gene="ilvM"
                     /locus_tag="KLK57_003126"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HBE0893429.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(8116..9762)
                     /locus_tag="KLK57_003127"
     CDS             complement(8116..9762)
                     /locus_tag="KLK57_003127"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HBE0893430.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVACSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGIKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(9765..9815)
                     /locus_tag="KLK57_003128"
     CDS             complement(9765..9815)
                     /locus_tag="KLK57_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893431.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="KLK57_003129"
     CDS             complement(9902..10000)
                     /gene="ilvL"
                     /locus_tag="KLK57_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HBE0893432.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            10353..11242
                     /locus_tag="KLK57_003130"
                     /pseudo
     CDS             10353..11242
                     /locus_tag="KLK57_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
BASE COUNT         2554 a         3090 c         2950 g         2648 t
ORIGIN      
        1 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
       61 gcgatcatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
      121 acctgcaatt ctttgatcag cagattcgtt gggccaaagc cgacctagta caacaagtca
      181 gtcgaaaccg attaaacaga gcaaggactt acagagggta tcttaatcaa tgtttgccac
      241 atgagttggt tgcagacata cagagggcgg tcatcaagtc aatcaatgcc atatcgctca
      301 taaattgaca ataaaataac catccccgat aatgactttg tagcccatag aggccctaag
      361 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtac
      421 cagcccctcc ctgactaaca attacgttag catctgcgga tcatgctaaa atcgccgcct
      481 gacaattatt cactcaaggc acgatcatgg caaaaacagc agcagcactg catatccttg
      541 taaaagaaga gaaactggct ctggatcttc tcgagcagat taagaacggt gccgatttcg
      601 gcaagctggc gaagaaacac tccatttgcc catcaggcaa acgcggcggt gatttaggtg
      661 aattccgcca gggtcagatg gttccggcgt ttgataaagt tgtgttctct tgtccggtac
      721 tggagccaac cggcccgctg cacacccagt tcggttacca catcattaag gtgctgtacc
      781 gtaactaata gcaaggcctt ctccaggaga aggccttgag tgttttctcc ctctccctgt
      841 gggagagggt cggggtgagg gcatcagcgc gcacttaacc cgcaactgca atacgtttca
      901 tatccgtcat atagccgcgc agtttcttac ctacctgctc aatcgcatgg cagcgaatcg
      961 cttcgttcac atcacgcagt tgcgcgttat ctaccgcacc ttccggaata gctttaccca
     1021 gatcgcccgg ttgcagctct gccataaacg gtttcagcag cggcacacaa gcgtaagaga
     1081 acagatagtt accgtactcg gcggtatcag agataaccac gttcatttcg tacagacgct
     1141 tacgggcgat ggtgttggca atcagcggca gttcgtgcag tgattcatag taagcagatt
     1201 cttcgatgat gccggaatca accatggttt caaacgccag ctcaacgccc gctttcacca
     1261 tcgcgatcat cagtacgcct ttatcgaagt actcctgctc gccgattttg ccttcatact
     1321 gcggcgcggt ttcgaaggcg gttttgccgg tctcttcacg ccaggtcagc agtttcttat
     1381 catcgttggc ccagtccgcc atcataccgg aggagaattc gccggagatg atgtcgtcca
     1441 tatgtttctg gaacaggggt gccatgatct ctttcagctg ttcagacagc gcgtaagcac
     1501 gcagtttcgc cgggttagag agacggtcca tcatcagggt gatgccgccc tgcttcagcg
     1561 cttcggtgat ggtttcccaa ccgaactgaa ttagtttttc tgcgtatgcc ggatcggtgc
     1621 cctcttccac cagcttgtcg aagcacagca gagagccagc ctgcaacata ccgcacagga
     1681 tggtttgctc acccatcagg tcagatttca cttccgcaac gaaggacgat tccagcacgc
     1741 ccgcacggtg accaccggtt gctgccgccc atgctttagc gatcgccatg ccttcgcctt
     1801 tcggatcgtt ttccgggtga acggcaatca gcgtcggtac gccgaatcca cgtttgtact
     1861 cttcacgtac ttcggtgcca gggcatttcg gcgcaaccat gactacggta atgtctttac
     1921 ggatctgctc gcccacttcg acgatgttga aaccgtgcga gtaacccagc gccgcgccgt
     1981 ctttcatcag tggctgtacg gtgcgcacta catcagagtg ctgcttgtct ggcgtcaggt
     2041 taaccaccag atccgcctgc gggatcagtt cttcgtaagt accaacttta aaaccgtttt
     2101 cggtcgcttt acgccaggac gcgcgcttct cggcaatcgc ttctttacgc agagcgtagg
     2161 agatatcgag accagaatca cgcatgttca ggccctggtt cagcccctgt gcgccacagc
     2221 cgacgataac cactttttta ccctgaaggt agctcgcgcc gtcggcgaat tcgtcgcgcc
     2281 ccataaagcg acatttgccc agctgtgcca gctgctggcg caggttcagt gtattgaagt
     2341 agttagccat ggtgattcct cgtgatgttg tgcttcttat tgttcggttc gcgtttgcga
     2401 gattgaattc actatatgac aggaaattta ttgcggaaat tgatatattc acaacgtcac
     2461 attgcaattt ctgcaacgtc aacatcgagg gctgcgccat tggatttacg cgatctgaaa
     2521 accttcctgc atctggcgga aagccgccat tttggccgca gcgcgcgggc gatgcacgtt
     2581 agcccatcca cgctctcacg gcagattcag cgcctggaag aagatcttgg tcagccgcta
     2641 tttgtgcgcg ataaccgcac ggtaacgctg acggaagcgg gcgaagagct gcgcgttttc
     2701 gcccagcaaa cgctgttgca gtatcagcaa ttgcgccaca ccatcgatca gcaagggccg
     2761 tcgctctctg gcgaattaca tatcttctgc tcggtgaccg ctgcctacag ccatctgccg
     2821 ccgattctgg atcgcttccg cgcggaacac ccgtcggtgg agatcaaact caccactggt
     2881 gatgcggcag atgcaatgga aaaagtggtc acgggagaag cggatctggc gattgccggg
     2941 aaaccggaga cgctacccgg cgctgtggcg ttttcgatgc tggagaatct ggcagtagtg
     3001 ctgattgccc ccgcgctgcc ctgcccggtg cgtaatcagg tatctgtcga aaagcccgac
     3061 tggtcaacgg tgccgtttat tatggccgat caggggccgg tacgccgccg cattgaactg
     3121 tggtttcgac gcaataaaat cagcaacccg atgatttacg ccacggttgg cgggcatgaa
     3181 gcgatggtat cgatggtggc gctcggctgt ggtgtggcgc tgttgccgga agtggtgctg
     3241 gaaaacagcc cggagcctgt acgcaaccgg gtaatgattt tagatcgcag cgatgagaaa
     3301 acgccgtttg aactgggcgt gtgtgcacaa aaaaagcggc tgcatgagcc gctgattgag
     3361 gcgttctgga ggattttgcc gaaccacaaa tgacgttgtc gcgcgggtag gcctgataag
     3421 cgaagcgcta tcaggcattt ttccctaacc cgccaaaaag aacctgaacg ccggattatt
     3481 ggtttcgtcg tggcaatcgt agcccagctc atttagccgg gtttcgaaat ccggttcatg
     3541 gtcgccgagt tcgaacgccg ccagtacgcg cccgtagtcg gtgccatggc tgcgatagtg
     3601 gaacaaagaa atgttccagt gcgtacccag cgtgttgagg aagcgcagca gcgcgcccgg
     3661 tgattccggg aattcgaagc tgtagaggcg ttcctgcaac ggatgtgacg gacgcccgcc
     3721 gaccatatag cgcacatgca acttcgccat ttcgtcgtcg gagagatcca ccacgctgta
     3781 gccgccgtcg ttgagcatct gcaaaatttc tttgcgctct tcgaggccgc ggctcaggcg
     3841 cacgccgaca aagatgcagg cgtttttggc atcggcaaaa cggtagttga actcggtgac
     3901 cgatcgcccg ccaagcagtt ggcagaattt gaggaagctg cctttttctt ccggaatggt
     3961 caccgccaac aacgcttcac gctgttcgcc cagttcgcag cgttccgaga cgtagcgcag
     4021 gccgtggaag ttcacgttgg caccggaaag aatatgcgcc aggcgttcgc cgcgaatgtt
     4081 gtgctgagcg atatattttt tcattcccgc cagcgccaac gcgccagagg gttccgccac
     4141 tgcgcgcaca tcttcgaata aatccttcat cgccgcacag atcgcatcac tatcgacggt
     4201 gatgatgtcg tcgagatact cctggcataa acggaaggtt tcgtcgccga tgcgttttac
     4261 cgcgacacct tcggcgaaca gtcccacgcg cggcagatca accggatgac ccgcatccag
     4321 cgctgctttc aggcaggcgg agtcttccgc ttctacggcg atcactttga tttgtggcat
     4381 cagttgtttg atcagcaccg caacgcccgc cgccagaccg ccgccgccga ctggcacaaa
     4441 tacgcggtcg agatgggcgt cctgctggag cagttccagc gccagcgtgc cttgcccggc
     4501 aatcaccatc ggatggtcga acggcggcac ccaggtgaac ccctgctgct gtgacagttc
     4561 gatcgcttta gctttcgctt cgtcaaagtt cgcgccatga agcagcactt cgccgccgaa
     4621 tccacgtacc gcatcgactt tgatgtcggc ggtggcggtt ggcataacga tcagggcctt
     4681 cacgcctaac cgcgcagaag aaaacgcgac gccctgcgcg tggttacccg cagaagcagt
     4741 gatcacgccg tgcgcttttt gttcttccgt caggcccgcc atcatggcgt atgcgccgcg
     4801 cagcttaaag ctgtgcactg gctggcgatc ttcgcgcttc accagaatga cgttatcaag
     4861 acgcgacgac agtttttcca ttttttgtag cggcgtaacc tgcgccgcct cgtaaaccgg
     4921 cgcgcgcagc actgctctta aatattcggc accttccgga gcaccggaca ggggttgcga
     4981 gtcagccatt attaaccccc cagtttcgat ttatcgcgca ccgcaccttt gtcggcgctg
     5041 gttgccaggc tggcgtaggc gcgcagggca aaggaaacct gacgttcacg gtttttcggc
     5101 gtccaggctt tgtcaccccg agcttcctgc gcttcacgac gcgccgccag ttcggcatcg
     5161 cttacctgta actgaatgcc acggttcggg atgtcgatag cgatcaggtc accatcttca
     5221 atcagaccaa tgctgccgcc gcttgccgct tccggtgaga cgtggccgat ggaaagacca
     5281 gaggtgccgc cagagaaacg accgtcggtg atcagcgcac aggctttacc gagacccatt
     5341 gatttcagga agctggttgg gtagagcatt tcctgcatgc cagggccgcc tttcgggcct
     5401 tcatagcgga tcaccaccac atcgcccgcg acaactttgc caccgaggat cgcttctacc
     5461 gcgtcgtcct ggctttcgta cactttcgcc gggccggtga atttgaggat gctgtcatcg
     5521 acgcccgctg ttttcacgat gcagccgttt tcagcaaaat taccgtagag caccgccagg
     5581 ccgccgtctt tgctgtaggc gtgttccagc gagcggatac agccgtttgc gcgatcgtca
     5641 tcgagagaat cccaacggca atcctgcgag aatgcctgtg tagtacgaat gcccgcaggg
     5701 cctgcgcgga acatattttt taccgcgtca tcctgggtca gcataacgtc gtattgttcc
     5761 agagtttgcg gcaacgtcag gccaagtacg tttttcacat cacggttcag taaccccgcg
     5821 cgatccagtt caccgagaat accgataaca ccaccagcac ggtgaacatc ttccatatgg
     5881 tatttttggg tgctcggcgc aactttgcat aactgcggga ctttacgcga aagtttgtcg
     5941 atatcactca tggtgaagtc gatttccgct tcctgcgccg ccgccagcag gtgaagtacc
     6001 gtgttggtag agccgcccat cgcgatatcc agcgtcatgg cattttcaaa cgccgctttg
     6061 ctagcaatat tgcgcggcag tgcactttcg tcgttttgct cgtagtaacg tttggtcaat
     6121 tcaacaatgc gtttaccggc attgaggaac aactgcttac ggtccgcgtg ggttgccagc
     6181 agcgagccgt tgcctggctg cgacaaaccc agcgcttcgg tcaggcagtt cattgagtta
     6241 gcggtaaaca tcccggagca ggaaccgcag gttgggcagg cggaacgttc aacctgatcg
     6301 ctctgggagt cagaaacttt cgggtctgcg ccctggatca tcgcatcaac cagatcgagc
     6361 ttgatgatct gctcggacag tttggttttc ccggcttcca tcgggccgcc ggaaacaaag
     6421 atcaccggaa tattcaggcg cagggaagcc atcagcatcc ccggggtgat tttgtcgcag
     6481 ttagagatgc agaccatggc gtcggcgcag tgggcgttga ccatatactc aacggaatca
     6541 gcgatcagtt cgcgagatgg cagtgaataa agcatccccc cgtggcccat agcaatccca
     6601 tcatccaccg caatggtgtt gaactctttg gcaacgccgc cagccgcttc aatttgttcg
     6661 gcgaccagtt taccgagatc gcgcagatgg acgtgacccg gtacaaattg ggtgaacgag
     6721 ttcacaaccg cgataatcgg cttgccaaaa tcggcatcgg tcattccggt ggcgcgccac
     6781 agcgcacgag cacccgccat attacgacca tgagtggtgg tggcggaacg gtacttaggc
     6841 atactttatt tactcccagt gtctgtctcg taaatgggac ggtaagtgcc gtcccatttt
     6901 ttgtatttat tgattaactt gatctaacca gccccattta tcttcggttt cgccagtgaa
     6961 gaggccgaag aaggcttgct gaatgcattt ggtaaccggg ccgcaacggc cttcacccac
     7021 ctgaataccg tctacactgc gcactggcgt gatttctgcc gccgtaccgg acataaacac
     7081 ttcatccgcc aggtacaggg attcgcgcga cagcacctgc tcacgcactt caattcccag
     7141 ctctttcgcc agtttgatga tggcatcacg ggtaataccc ggcagcgcgg atgaggtaaa
     7201 cggtggggtg aacagcacgc cgtctttcac ttcaaacagg ttttcacctg cgccttcaga
     7261 gatgtaaccg ttcacatcca gcgcgatacc ttcctgataa ccgtggcggc gcgcttcgct
     7321 acccaccagc agggaagaga ggtagttacc accggctttt gccgccgtcg ggatggtgtt
     7381 tggtgctgcg cggttccagg aggaaaccat cgcatcgatc ccctgctcca gcgcttctgc
     7441 gcccagatac gctccccacg ggaaagcagc gataatcacg tcggttgagt atcccgctgg
     7501 cgggtttact cccatgccaa catcaccgac gaagatcagc ggacggatat aggcgctggt
     7561 gagattattt ttgcggatca cgtcacgaca agcttccatc agctcatcaa tgctctgcga
     7621 aaccgggaag cgatagattt tggcggagtc atgcagacgc tgcatatgct cacgatggcg
     7681 gaatacaacc ggtcctttgt gcgagtcgta gcaacggatg ccttcaaaaa ccgaagtgcc
     7741 atagtgcagc gcgtgcgaca tcacatgcac cttcgcgtct tcccagcgaa ccatctcccc
     7801 attgaaccaa atgtaatcag ctttcttcgt ggtcattttt atattccttt tgcgctcagg
     7861 cgcggatttg ttgtgatgtg gttgtgctct ggcagatggc aacgtgtgcg acgtccacca
     7921 gtttatttaa ctgactaaac agtaagtcga ccgaccgtgg gctggcaacg gtcaattcga
     7981 tatttatatt ttgtgcatcg ctggcggcgg ccatattcat tgagcagacg tggaaaccac
     8041 gatgacgcac cacgcgtaaa acacgttcta aggtttctgg attgaagcga gccgatacat
     8101 tgacctgatg ttgcatcatg ataatttctc caacatttct gaattactgg cgccaggcgg
     8161 caccagcggc cagacgttct caagttcgtc gattgagaca tgaagcaggt atggcccatc
     8221 actgttcagc atggtgtcga gtgccgcttc aacctggtct ttacgggtga tgtgttggcc
     8281 agggatgccg aaggcgctgg ctaacatgag gaaatcgggg ttatcagtaa gggtggtttc
     8341 gctgtatcgt tcctgaaaaa acagttgctg ccattgtcga accatcccta accgttggtt
     8401 atcgagtaag acgattttca acggtaactg cttgcgtttt acggtgccca gctcttgcac
     8461 attcatcatg aaagagccgt caccggagat acagacaacg gtatcgttcg gtcgcgcgac
     8521 ttgtgcgcca accgccgccg gtaaaccaaa acccatggta cctaagccgc tggaggtgat
     8581 gaaattttcc gggcgagtgt gggcgatgtg ctgcgcagcc cacatctggt gctgccccac
     8641 atctgtggtc acgacgcaat ccgcaggttt acgatccgac agttgtttta acaacaacgg
     8701 cgcgtagata gcgtcaccgg gatggtcgta acgccaggaa tgttcatcac gcagctgcgc
     8761 gcagtattgc tgccagtcat tgatatttaa cggctgctgt aatgctggta acagagcatt
     8821 taaatcaccc tgtaatgcca catgtgcctg acgcagcttg ttcatttctg ccgggtcgat
     8881 atccatatgg ataacactgg cgtgtggtgc gaaagtattc agtttgccgg tcacccggtc
     8941 atcaaaacgt gcgcccacgg cgatcagcag gtcacactcc tgcaccgcga agtttgccgc
     9001 tttgatgccg tgcatcccca gcatgcccag atagtacgga taatctgctt ctactgcgcc
     9061 cagccctttc agcgtacagg tggcaggcat ttttgtggca gcgagaaatt cacgcaaagc
     9121 cggaactgcc tgcgccatac ccacgccacc gccaacgtac agcatcggtt tttgcgcttt
     9181 tgccagcatc tggcgcgctt gctcaacttc ggcatgtggg aaagtcactt cgttttcaac
     9241 ggtggtgaac cacggttcca ggtcaccgct ggctaactgg atatcttttg ggatatcgac
     9301 cagaaccgga ccaggacgac ctgagcaggc aacgtcgaat gcttcagcca tgatgcgcgg
     9361 caactcttcc agcgactgca ccagaaagct gtgcttggta caggctaacg acaatcctag
     9421 gacatccact tcctgaaatg cgtcagtgcc gataaacggt gcggacactt gaccggtgat
     9481 ggcaacaaca gggatggaat ctaacagtgc gtccgcaagc ccggttatca ggttggttgc
     9541 gcccggacca gacgtggcga tacatacgcc agttttgccg gtagcacggg cataaccgat
     9601 agccgccatt gccgcaccct gctcatgtcg gcatagcaag tgctccacgc cgccgtcata
     9661 caatgcatcg taaaccggca taattgcgcc acccggataa ccgaaaacgg tgttcacacc
     9721 ctgtgcccgc aacgcatgta ccacccactg tgcgccattc atagttagtt ccccgtcctg
     9781 aatcttgaga aacagaattt tgtgctgtta ttcattgtct gctcctcggt tatgttttta
     9841 aggtcaaaaa aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc
     9901 tctaagcctt tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc
     9961 taatcacgac taggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt
    10021 gttcgaagga atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg
    10081 gcctattttt atgatagctt taataatttc aatagaagtt gttgtttttt aaaaagaaaa
    10141 tagtgagtga aaaaaattca atttcagtat aaatgttgtt cccttcctcg tagttcatcc
    10201 tttgtccgct gcatttttgc gagcatcatt ccaccgccag aacaactcaa cttcacttgc
    10261 acaaataaac aggaatcagc cactaaaatt cacgaattag cgggctacag cagcgatatc
    10321 ttatgcacat aatggtgact caaggagggc ttatgtcact gtcaattgtt catacccgcg
    10381 cagccctggg agtaaatgcg ccccctgatc actgttgagg tacatatcag taaaggtcta
    10441 cccggcttaa cgatggtggg cttaccagaa acaactgtaa aagaagctcg cgatcgcgtg
    10501 cgcagcgcca ttatcaatag cggatatgaa tatccggcga aaaaaatcac catcaacctt
    10561 gctccagccg atctgccaaa agaaggggga cgatatgatt tacctatcgc cattgcgttg
    10621 ctggcggcct cagaacagct tacagccaat aagttagatg aatatgaatt agtcggagaa
    10681 ctggctctta caggcgctct gcgtggcgtt cccggtgcaa tctccagtgc aactgaagct
    10741 attaagtcgg gcagaaaaat tatcgtcgcg aaagataacg aagatgaagt ggggctaatt
    10801 aacggtgaag gatgcctgat agccgatcat ctgcaagctg tctgtgcgtt tctggaaggt
    10861 aagcacgctc tcgaacgccc gaaaccaact gatgcagtat cccgggcgct acaacatgat
    10921 ctcagtgatg ttgtcggtca ggaacaagga aagcgaggac tggaaattac cgccgctggc
    10981 aggcacaacc ttttactgat tgggccgccg ggaacaggta aaacaatgct cgccagccgt
    11041 attaatggcc tgttgccaga tttaagcaat gaagaggcac tggagagcgc tgcgatatta
    11101 agtctggtaa atgctgaatc agtacaaaaa caatggcggc agcgcccgtt ccgctcacct
    11161 catcacagtg cgtcgttaac tgcgatggtg ggcggtggtg caattccagg gccaggtgaa
    11221 atttcgttgg cgcataacgg cg
//
LOCUS       DAEEKQ010000128        11021 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.129, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000128 DAEEKQ010000000
VERSION     DAEEKQ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11021
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.129"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(92..403)
                     /locus_tag="KLK57_003131"
                     /pseudo
     CDS             complement(92..403)
                     /locus_tag="KLK57_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            complement(422..2299)
                     /gene="bglF"
                     /locus_tag="KLK57_003132"
     CDS             complement(422..2299)
                     /gene="bglF"
                     /locus_tag="KLK57_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HBE0893433.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADALGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDVTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(2433..3269)
                     /gene="bglG"
                     /locus_tag="KLK57_003133"
     CDS             complement(2433..3269)
                     /gene="bglG"
                     /locus_tag="KLK57_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HBE0893434.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKCAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(3555..4280)
                     /gene="phoU"
                     /locus_tag="KLK57_003134"
     CDS             complement(3555..4280)
                     /gene="phoU"
                     /locus_tag="KLK57_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HBE0893435.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4295..5068)
                     /gene="pstB"
                     /locus_tag="KLK57_003135"
     CDS             complement(4295..5068)
                     /gene="pstB"
                     /locus_tag="KLK57_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HBE0893436.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5159..6049)
                     /gene="pstA"
                     /locus_tag="KLK57_003136"
     CDS             complement(5159..6049)
                     /gene="pstA"
                     /locus_tag="KLK57_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HBE0893437.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSVPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6049..7008)
                     /gene="pstC"
                     /locus_tag="KLK57_003137"
     CDS             complement(6049..7008)
                     /gene="pstC"
                     /locus_tag="KLK57_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HBE0893438.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7095..8135)
                     /gene="pstS"
                     /locus_tag="KLK57_003138"
     CDS             complement(7095..8135)
                     /gene="pstS"
                     /locus_tag="KLK57_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HBE0893439.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKEIGNKVNYQGIGSSGGVKQIIANTVDFGASDSPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDETIAKLNPGLKLPSQNIAVVRR
                     ADGSGTTFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTDENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8383..9456)
                     /locus_tag="KLK57_003139"
     CDS             complement(8383..9456)
                     /locus_tag="KLK57_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0893440.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHRTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKYNCNRIEPLSISSGGKA
                     IVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDITAPQSCEIN
                     NLQPVYFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEP
                     NTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPAS
                     LTGARPAPGQFTATATITVEIVR"
     gene            complement(9467..>11021)
                     /locus_tag="KLK57_003140"
     CDS             complement(9467..>11021)
                     /locus_tag="KLK57_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE0893441.1"
                     /translation="VTIKEEDGSEQRFTQPYASLAILKREGQTDVDVSVGELRDEDGF
                     TPDVLQAQILHGFSHGITLYGGMQAAENYGSAALGVGKDLGALGAISFDVTHARANFS
                     HDDTETGQSYRFLYSKRFDDTDTSLRLVGYRYSTEGYYTLNEWASRRNSPEDFWETGN
                     RRSCVEGTLTQSLGRDYGNLYLTLSRQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSY
                     SNTARQGTGNNHASDNTSEQIYMLSLSVPLLGWWGNSYATYSVSQNDNSGSSHQLGLS
                     GTALERNNLSWNLMQSYNSHDDEVGGNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRG
                     GILAHSEGVTLSQELGETIALVKAPGAAGLEIDNMRGAATDWRGYTVKTQLNPYDENR
                     VAISDNYFSKSNIELDNTVVTMVPTRGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGA
                     IAAIQDASLADSGIVGDRGELYLSGLPEKGQVTLSWGENASTKCIFNYSLSTPESESG
                     LIEQGVTCH"
BASE COUNT         2886 a         2848 c         2497 g         2790 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca acgctaaccc cgcttcattc ggttcggctt
      121 cgtcgccctg agggaaaatt cgcgcccagg caatggaaat acgcagacag gtgaagccca
      181 tctcggcaaa taacgcgata tcttccgggt aacggtgata aaaatcgatg gcgacatctt
      241 tgatattctc tttccccagg atgcgcggtt ccatttttcc cataacgcca tgaggctgta
      301 aatctgaggt cgtgctccct ttgccatctt ccagccaggc accttccacc tgattggcag
      361 ctgttgcgcc accccaaaga aatgtttctg gaaatgcttt cataattaac tccttttatc
      421 gttagcgaat gatggataac agcggttcac ctgcgcttat ctgcgccgtg ccgtggggta
      481 atacgtctgt aaaatcatcg ctattactga ttaataccgg cgtcgtcaca tcaaatccgg
      541 cctcgcgaat agcagggata tcaaaagaaa tcagccgatc gcctgtattg accttgtcac
      601 ccacgttgac gtgagcggaa aagaatttgc cgtccagttt tacggtgtcg ataccgacat
      661 gaatcaggat ctccacacca tcatctgact caatgccaat ggcgtgtaat gtggcgaaca
      721 acgaagcaat tcgacccgca accggagaac gcacttcacc aaccgagggc agaatggcga
      781 tacctttacc caacaggcca ctggcaaacg tggtatcagc gacgtgaatg agcggcacaa
      841 tctctcccgt catcggtgaa cagataccgc cctgctcagg tggtgtaata acctctggtg
      901 ttttctcttg cggggcaccc tgcgctggct gacgtttagc ggtgatgaaa tgaagcatca
      961 ccgtaccgac aaatgcgcaa ccgatggcaa tgacaccgcc aataacgctg gcccagacgg
     1021 tgaaatcaat tcccgttgac gggatggttt gcatgaaggt gaaaatactt ggcaaaccaa
     1081 aggagtacac tttcgtttgc gcgtagccaa tgatggtggc ccccaaagcc ccactgatac
     1141 aggcgataac aaaggggtac ttacgcggca gattaacgcc atataccgct ggttcggtga
     1201 taccaaacag gcccgtcaac gccgctgatc ccgccaccac ttttttctgc gcatcgcgtt
     1261 cgcagaggaa gacgccgagc gccgccccga cctgcgccat aatggcgggc attaacagcg
     1321 ggatcatggt gtcgtagccc agcacggtga agttattgat acacagcggc accaggcccc
     1381 agtgcagtcc gaacatgacg aagatttgcc agaagccgcc cattaccgcg cccgcaaatg
     1441 caggaaccgc ctgataaagc cagagataac cggcggcaat cagttcgctt atccaggttg
     1501 atagcggccc caccagcaga aaggtgacgg gtgtgataac catcagacat agcaatggtg
     1561 tgaagaaatt tttgattgcc gacggtaacc acgcattaag tcggcgttcc agaatgctgc
     1621 acaaccaggc agaaaaaata atgggaataa ccgatgacga gtaattcaac aatgtgaccg
     1681 gaatacccag gaaatccagc cccagcgcat ccgctttttg cccgttctcg aaagcagtca
     1741 gaattaatgg atgcactaac gctccaccaa tcaccatggc agtaaatgga ttaccgccaa
     1801 agcgtttccc cgcggtgtat cccaggatta tcgggaagaa ccaaaacaag gcatcactgg
     1861 cgctgaataa aattaaataa gtaccacttt gttcggtcgt ccactgaaaa gtgagcgcca
     1921 gagccagcat acctttcagg atcccggttg ccgccatcaa accgatcaga ggcgtaaaaa
     1981 tacctgaaat aacataaaca aagcggttta gcagattacc tttctcatca ttttccggtg
     2041 cctgttgcgc tttttcgcca aggcctgcca cgctgttgac cgccaggaag acatcggcca
     2101 catggttacc tatgaccacc tgaaactggc caccgctttc caccaccata ataataccgg
     2161 gggtgttttt cagtacctca gcttgcgctt tgctttcatc ctttaactta aaacgtaatc
     2221 gcgttgcgca atgcatcaga ctaacaatgt tatctgcgcc cccgactcct gcgactattt
     2281 ttctggctaa ctccgtcata acttgccctc taccgctttg cggcaaaact ccaaaaaaaa
     2341 aactgaaaaa aacggcctga cgtgaatcaa gcaatttttt tcaggttttg cccgcttagt
     2401 gcggtaacaa tcctttactc agtaataata tttcagtgtt ctttgcgcac gcgctctata
     2461 tttatggcta aaaacataat ctctgcgggt gaaattttac gttgatactg caaaccaata
     2521 aaaatggcga tccgttccgc acattgccat gcttgcgggt aattttgttt tactgcttgt
     2581 tgtaatgatt catcactatc gttaattgaa gcatgttcaa gaatacgcca ggataaaaac
     2641 ttcagatgtg taaccagtcg ctgataactc aagctttctt cctggtaatt aaggctgaac
     2701 tgaaatttta ttaattgcag catttcgcgc attaactgcg tgacacctgc aacatcctcc
     2761 atatttccgc tcatttgggc actgaccaga tgcatggcaa taaagcccac ttcatctttc
     2821 ggtaactgca cgcccaaccg tttatcaata atggttaacg cttcttcccc tagctggaac
     2881 tctttcgggt aaagccgctg gatatcccac agcaacgggt tgggcagcaa cacgttttgc
     2941 tgaaagcgtt taatcgcaaa ctggcaatgg tcagttagcg agatataaat actgtcctgt
     3001 aattttccca agcgctcctg cgctaaagag ataatacgat cacaggttgc catcacctca
     3061 agaggaatat gacttaagag ttcgcttaat cgcccgttca gttcatgact gctcaaggca
     3121 tactcttttt ctattccact tgagttaatt ctttcgccag cgcatttttg aaagccaatt
     3181 cctcgcccca tgacgacttt ttcccgctgt tgatcatcaa taaccaccac aacattattg
     3241 ttgagaattt tggtgatttg catgttcata gcaaggacct tttttataaa caaaaaaaac
     3301 cgacttcacc agtattctct ggttatgtca ggttttgcct gcgaatgcag taacaatcca
     3361 atcatttatt aatgtttttt ataacgaaca tccaggttcg gaaattaatt taattgcgtg
     3421 cagagaaaat agcaatgcgc tattgataaa aatatgacca tgctcgcagt tattaacttt
     3481 gtgtaatttt aggaatttat aaagttatat ataacaaatc ccaataatta agttattggg
     3541 atttgtctgg tgaattattt gtcgctatct tttcccgcca gcagtttatc cagctcatca
     3601 ccgcctacat gacggaaatc ctgccccttc acgtagtaga agataaactc acaaatattc
     3661 tggcagcggt cgccaatacg ttcgatagaa cgcgcgcaga acagcgcagt aagtacgctc
     3721 ggaatggtac gcgaatcttc catcatgtag gtcatcagtt gacgaacaat accttcgtat
     3781 tcctgatcga cttttttatc ttcacgataa atacgtaccg cttcgtcaat gtccatccgc
     3841 gcgaacgcgt ccagcacgtc gtgcagcatc tggatggtat gacggcccag cgactccaga
     3901 cttaccagca acggctgatg ctgctgggag aatttctcca gcgcagtacg gcagattttg
     3961 tccgccacgt cgccaatacg ctccagctcg gcaatggttt tactgatcac cataaccagt
     4021 cgcaggtcgc tcgccgtcgg ctgacgtttg gcgataatgc gcacgcaggc ttcatcgatc
     4081 gccacttcca tcatgttgac gttcttgtcg ccttcgatca cgcgcttcgc cagatcgctg
     4141 tcctggttat gcatcgcggt gattgcatca gaaagctgct gctccaccat gccacccatg
     4201 gtcatcacct gcgtgcggat actttccagt tcggcgttga actggccgga aatatgttta
     4261 ttaagattga gactgtccat aacgcactcc tgaattaacc gtaacgaccg gtgatgtagt
     4321 cttctgtttg tttcttcgct ggcttggtga acagatcgtc cgtgttgctg aactcaatca
     4381 attcgcccag gtacataaac gccgtgtggt cggaacaacg cgcagcctgc tgcatgttgt
     4441 gggtaacgat caccacggtg taatcctgct tcagttcggt gatcagctct tcaatacgcc
     4501 cggtagagat ggggtcgagc gccgaacacg gttcgtcgag cagcagcact tccgggcgaa
     4561 tggcgatacc acgcgcaata cacagacgct gttgctgacc accagagaga gagtaaccgc
     4621 tctggtgcaa tttatctttg gtttcgttcc acaatgcggc tttggtcaat gcccactgca
     4681 cgcgctcgtc catgtcggca cgggagagct tctcaaacag acgaacacca aaagcgatgt
     4741 tgtcgtagat ggacatcggg aacggcgtcg gtttctggaa caccatgccc actttcgcac
     4801 gcagcagtgc gatatcctga gagttggtca ggatgttgtc gccatcaagc agaatttcac
     4861 cttctgcgcg ctgctccgga tacagttcaa acattttgtt gaaggtacgc agcagcgtcg
     4921 atttaccgca gccggacggc ccgataaacg ccgttacctg gtttttagcg atatccaggt
     4981 tgatgttttt cagggcatgg aatttgccgt agtagaagtt caaattacga acctgaattt
     5041 tactcggggc agtttcaacc atactcattg caatcttttc ctcatcgtac ctgatgcttt
     5101 tcgctaatca ggcttaccgt aggccgggta aggcgttcac gccgcatccg gcaaagtgtt
     5161 aaccgtgttt attcttcgca aaaacaacgc gcgccagaat gttcagcagc aggacgcaca
     5221 gggtaatgat caataccccg gcccaggcca gttgttgcca ttccgcaaac gggctcatcg
     5281 caaacttaaa gatcgtcacc ggcaggttgg cgatcggctg catcatgtcc gtgctccaga
     5341 actggttgga gagcgcggta aacagcagcg gcgcggtttc accggcaata cgggcaatcg
     5401 ccagcaggat accggtcata atcccggaca ccgacgcttt cagcgtaatc gcggagatca
     5461 tcttccactt cggtgtaccc agcgcataag ccgcttcacg caggctgtcc ggcaccagtt
     5521 tcagcatgtt ctcggtggtg cggataacga tcggcacctg caacagcgcc agcgcaatta
     5581 cgcccgccca gccggagaag tgctccatct gtgccaccac gatggtgtag acaaacagac
     5641 caaccacaat cgacggcacg gagagcagaa tgtcgttaat gaagcgaatc acttctgcca
     5701 gccaggattt acgaccatat tccgccagat aaatccccgc cataatgccc agcggcgtac
     5761 cgaataccgt ggcccacaaa attaacagcc cgctacccgc cagggcgttc gccagaccac
     5821 caccttccgt attgggcggc ggcgtcattt cagtgaacag cgccagcgac ataccgtcga
     5881 taccgcgagt gatggtggac attaaaatcc agatcagcca gaacaggccg aaggccatcg
     5941 tcgccatcga gagcgtcagc gcaatacggt ttttgaggcg gcgacgcgcc tgcattttgc
     6001 ggcgagattc agccagcgcc gcagtggttt gcatttcaac catagccatt agcgtgcccc
     6061 ctcattctta gccaggcgca taatcataaa cttcgatgcg gcgaggacga tgaaggtaat
     6121 cacaaacagg atcaggccca gttccatcag tgcggcaacg tgcagaccgg attccgcttc
     6181 cgcaaattcg ttcgccaacg cagaggtgat actattgccc ggcatgtaca gcgaggcgct
     6241 gtcgagctgg taggtgttac cgatgataaa ggtcaccgcc atggtttcac cgagcgcgcg
     6301 gcccagcccc agcatgatgc cgccgataac accatttttg gtgaacggaa gaacgatacg
     6361 ccagataact tcccaggtgg tgcagccaat accgtaggcc gactctttca tcatcaccgg
     6421 ggtttgttcg aacacatcac gcattaccgc cgcaatgtac ggaataatca tgatggcgag
     6481 gatcacgcct gccgcgagga taccgatacc aaatgcgggg ccagagaaca gcgcgccaac
     6541 aatcgggata ttcgacatga tattgccgac cggctcctga aagtaaacgg cgaacagcgg
     6601 cgcaaagata aacaggcccc acatgccgta aacgatactt ggaatggctg ccagcagctc
     6661 aatggcgata cccagcgggc gtttcagcca gccaggcgca agctcagtca ggaacagggc
     6721 gataccgaaa ctcaccggga cggcgatcag cagcgcgata aacgaagtca ccaacgtacc
     6781 gtagatcggc accagcgccc cgtagatatc gttcggtgca tcccactctt tggtccatag
     6841 gaaagccaga ccaaatttct gaatgctcgg ccaggaggag atgatcagag agacaataat
     6901 gccacccaac atcaatagca caatcagcgc cgccagtttt accagcacgc tgaaaattat
     6961 gtcgcccttt ttacccggtg ggttaaaagc aggcttggtt gcagccataa gttactcttc
     7021 agttaaacgc gtttacaaag tcattttgta atgccggatg cggcgtaaaa caccgtaccc
     7081 ggcctggagt tttattagta cagcggctta ccgctactgt ctttaatatt ggtcttccac
     7141 gcagcgcgaa cctgttcaac tacactatcc ggcaggctgg cgtaatccag gtcgttcgcc
     7201 tgtttagccc cggttttgta cgcccagtcg aagaatttca gcacttctgt gccttgttct
     7261 ggtttcttct gatctttgtg gatcagaatg aacgtggtag aggtaatagg ccatgcatct
     7321 tcgcctttct ggttggtcag atcctgagcg aaggttttgc tccagtctgc accttttgct
     7381 gcattagcga agttttcgtc ggtcggacta accggtttac catcagcgga gatcagtttg
     7441 gtgtacgcca ggttgttctg cttcgcgtaa gcatattcaa cataaccaat tgcacccggc
     7501 agacgctgaa cgaacgcggc gataccgtcg ttacctttac cgcccagacc gatcggccat
     7561 tttacggtag agccagtacc aacgttgttt ttccactctt cgttcacttt cgccaggtag
     7621 ctggtgaaga cgaaggtagt cccggagcca tctgcgcggc gtactacagc aatgttttgt
     7681 gaaggcagtt tcagacccgg attcagtttg gcgatggttt catcatccca cttcttgatt
     7741 ttgcccaggt agatgtcgcc gagggtttta ccatccagca ccagttcgcc agacttcagc
     7801 cctggaatgt taaccgccag caccacgccg ccaatcacgg tcgggaactg gaacagacct
     7861 tcctgagcca gtttttcgtc agacagcggc gagtcagagg caccaaaatc aacggtatta
     7921 gcgataatct gttttacgcc accggaagaa ccgataccct ggtagttaac tttattaccg
     7981 atttctttct ggtaagtgtc agcccatttg gcatacaccg gcgcagggaa ggttgcacct
     8041 gcacctgtca ggcttgcttc tgcaaacaca gagaaagcgc tcatcgataa ggtcgcggcg
     8101 acaacagttg cgacggtggt acgcataact ttcataatgt ctcctgggag gattcataaa
     8161 gcattgtttg ttggctacga gaagcaaaat aggacaaaca ggtgacagtt atatgtaagg
     8221 aatatgacat ttttatgaca gaaaggaatt aacaaataac ttactgattt taaatgtgaa
     8281 gatgaaaatc ccacatgtca tctttttgtc atgatgcatt taaaagtgat aaagaaatgg
     8341 gtgagatgac aacgatgtga tgaacaatcg ttgtcagacc tattagcgca caatctcaac
     8401 agttatcgtc gctgttgcag taaattgccc cggggctggg cgagctccag taaggctcgc
     8461 aggagcagct gaaaaagtta cactacctga tttttcacca tacactgttg atttacccat
     8521 gtctgttggc aactcaccac cgttcacttt gatttcctta ttatttttgt cgtaaatttt
     8581 tatccccacg ttagagtttg aagttacgac cattgaattg tccgtattgg gttcggtagc
     8641 ataaaaagac gtactgatat catcggtatt cagtatcccc aggttttcac actcaatagt
     8701 gactgtttta gtaattttat gtgttgttac cgcacttcca acaacagatg aaaaatctgc
     8761 agctggtatt tctttaaaat caaaataaac tggctgtaaa ttatttattt cgcaactttg
     8821 cggagctgta atatcgcccc gaatataaac atcagccatc ggctgtgagt aatccaccgg
     8881 ggtgtcacgg ctaattgttc catacaatgt cactatcttt gtttcaggaa taatcaattt
     8941 tcctgcaaat gtcttcttga tataaaaata tactatcgct ttacccccac tggatatact
     9001 taaaggttca atgcggttgc aattgtactt tgtattacca gacgggttgt tagcatgata
     9061 ttcaaaaggt gcattcacat acccaacccc aagaatatct atagcaatcc caacatcaag
     9121 gttcggatta agatcataat aattaatatt agagtgaacc ccagcataag ttaatgcctg
     9181 gtttattttt gacgtataat agatataggg gaattctcct acattaccag tatcacaatg
     9241 acagcgtcca ggatatgagg atccattagt tacagtggta ttaaatgtcg ttccaacctt
     9301 attttcgttc gcggtaataa gatatcccga gaaatcaagc gtacggtgaa atacgccatt
     9361 atcaggggta cagatacctt taggagctgc aagtgctaca ctacttaacg agagcataaa
     9421 cacagcaaag caccactgtt tgatatactt gttcataaaa atccttttaa tgacatgtca
     9481 caccctgttc aattaatccg ctctcacttt ctggtgtcga aagtgaataa ttgaagatgc
     9541 attttgttga ggcgttttct ccccaggata acgtaacctg tcctttttct ggtagaccag
     9601 aaagataaag ttcgccacgg tcaccgacaa ttcctgaatc tgccaaactt gcatcttgta
     9661 tcgcagcaat ggctccaaaa ggtaccggtt taccatttgc atttaacact ctgaagagaa
     9721 cgcgataacc cacatgagtc acaaactccg ctttaaccac tgcaccacgc gtgggaacca
     9781 tcgtaacgac ggtattatca agttctatat tcgacttcga gaaatagtta tcgctgattg
     9841 ctacccgatt ttcatcataa gggtttagct gtgtcttgac cgtatagccg cgccagtccg
     9901 tcgcagcacc gcgcatatta tctatttcta acccggcggc cccaggtgct ttaacaagag
     9961 caatagtttc acctaactcc tgacttaacg ttaccccttc gctgtgtgcc agaattcccc
    10021 ctctgatacc ataattcagc ctctgggaat tttggctgta gttatagctg ccgttcaccg
    10081 tgccataaga gccatcatag gtcagggaca tattaccgcc aacctcatca tcatgactgt
    10141 tataggactg cattaaattc catgaaaggt tatttctttc cagcgccgta ccgctgagtc
    10201 cgagttgatg tgagctaccg gaattatcgt tttgcgaaac agaataggtg gcgtaactat
    10261 taccccacca gcccaataaa ggaacagata aagagagcat gtagatctgc tcactggtgt
    10321 tatcactggc atggttgttc cccgtcccct gtctggcagt attggaataa ctccaggaga
    10381 cgttccacga gagacgcttc cagctactgc tgtagccaaa ttgcattaat cgttcgacat
    10441 catcggtatg ccagtattgt tgccggctta atgtcaggta taaattgcca taatctctcc
    10501 ccaacgactg cgttagcgtc ccctccacgc aactacgtcg gttacctgtt tcccaaaagt
    10561 cttcagggct gttgcgccgc gatgcccact cattgagggt atagtagccc tcggtggagt
    10621 aacgatagcc aaccaagcgc aagctagtgt ctgtgtcgtc aaatcgtttt gaatagagaa
    10681 agcgatatga ctgacccgtt tctgtatcat catggctaaa attcgcacga gcatgtgtca
    10741 catcgaaaga aattgcgccc aaagcgccaa gatctttacc gacacccaga gctgcagaac
    10801 cataattttc agcagcctgc atacctccat ataaagtgat cccgtgggaa aaaccatgaa
    10861 gtatttgcgc ctgaaggacg tccggtgtaa atccgtcttc atcgcgcaat tcacccacgc
    10921 tgacatcaac atctgtctga ccttcacgtt taagaatcgc taatgaagca taaggttggg
    10981 tgaatcgttg ttcgctaccg tcttcttctt tgatggttac g
//
LOCUS       DAEEKQ010000129        11003 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.130, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000129 DAEEKQ010000000
VERSION     DAEEKQ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.130"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..280)
                     /locus_tag="KLK57_003141"
     CDS             complement(<1..280)
                     /locus_tag="KLK57_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0893442.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSR"
     gene            complement(447..1187)
                     /locus_tag="KLK57_003142"
     CDS             complement(447..1187)
                     /locus_tag="KLK57_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HBE0893443.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(1280..1915)
                     /gene="argO"
                     /locus_tag="KLK57_003143"
     CDS             complement(1280..1915)
                     /gene="argO"
                     /locus_tag="KLK57_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HBE0893444.1"
                     /translation="MFSYYFQGIALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(2054..2914)
                     /gene="mscS"
                     /locus_tag="KLK57_003144"
     CDS             complement(2054..2914)
                     /gene="mscS"
                     /locus_tag="KLK57_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HBE0893445.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(3272..4351)
                     /gene="fbaA"
                     /locus_tag="KLK57_003145"
     CDS             complement(3272..4351)
                     /gene="fbaA"
                     /locus_tag="KLK57_003145"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HBE0893446.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(4566..5729)
                     /gene="pgk"
                     /locus_tag="KLK57_003146"
     CDS             complement(4566..5729)
                     /gene="pgk"
                     /locus_tag="KLK57_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HBE0893447.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETASATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(5779..6798)
                     /gene="epd"
                     /locus_tag="KLK57_003147"
     CDS             complement(5779..6798)
                     /gene="epd"
                     /locus_tag="KLK57_003147"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HBE0893448.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7170..7601
                     /locus_tag="KLK57_003148"
     CDS             7170..7601
                     /locus_tag="KLK57_003148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE0893449.1"
                     /translation="MMTLPYPYSSPARFYALRLLPGQEVFSQLHAFAQQHQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            7624..8202
                     /locus_tag="KLK57_003149"
     CDS             7624..8202
                     /locus_tag="KLK57_003149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HBE0893450.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            8203..8910
                     /locus_tag="KLK57_003150"
     CDS             8203..8910
                     /locus_tag="KLK57_003150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HBE0893451.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVAWLMFSLGAGLWLVHGGWLTEWLSGTPRSPERWTHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIILLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            8898..9575
                     /locus_tag="KLK57_003151"
     CDS             8898..9575
                     /locus_tag="KLK57_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HBE0893452.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAETEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWALERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            9569..10246
                     /locus_tag="KLK57_003152"
     CDS             9569..10246
                     /locus_tag="KLK57_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HBE0893453.1"
                     /translation="MLTLNQISYRWPSAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(10218..10931)
                     /locus_tag="KLK57_003153"
     CDS             complement(10218..10931)
                     /locus_tag="KLK57_003153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HBE0893454.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLVQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(10928..>11003)
                     /locus_tag="KLK57_003154"
     CDS             complement(10928..>11003)
                     /locus_tag="KLK57_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HBE0893455.1"
                     /translation="ARFSGMVKTALTLAVTTTLKELTP"
BASE COUNT         2626 a         2921 c         2741 g         2715 t
ORIGIN      
        1 ctcgtgaacg acgccctgag ttatggattt cattatcaat cgcagaaagt cgttgataaa
       61 ccgggaacag tttttgatac agttcctgac cggctttatt gaggctgata ttattatctt
      121 tgcgggtaaa tagcgtatag ccaatttgtt cttccagcgc ggcaatgctt ttaccaaatg
      181 gcgaagccgt catatggatt ttttctgccg cccgggcaat attattggtt tgcgctaata
      241 aaatgaaatt gcgcatcttt ttcgaaataa aaatgtccat cccccctccc cgcatccttg
      301 tccgcttaac cgtttcagtc agcctatcct tgatgaaacc gcgagcaaag ataggtgatt
      361 acgtcatggt tttacagaaa attacagaaa aaggaggcaa tatcgggtaa aggcattagc
      421 ccgacgaata cgtcgggcta caagtattat tgtgctgcag gtgttttagc gggttgttga
      481 tccacaggtt ctaactggaa gaccacatcg acctgatcat caaactgaat agcggcctgc
      541 tcgtaagttt cctgggcaga caccggcgcg gcatcggctt tcatcatccg caccattggg
      601 ctgggctgat agttggaaac atggtagcgc acgctatata ccggccccag tttacgatga
      661 aagccgttcg ccagttcctg cgcctgatga atcgcgttat caatcgctgc cttacgcgct
      721 ttgtctttat aggcatccgg ctgcgccacg cccagcgaca cagaacgaat ttcgttaaga
      781 cccgccttca gcgcgccatc cagcaaggaa ttcagtttgt ctaactgacg gagcgtgact
      841 tccaccgttc tcacagcgcg gtagcctttc aggatacttt taccatcctg ataatcataa
      901 tctggctggg tgcgtaagtt cgctgagctg atatcttttt tcgcgatctg attgagttca
      961 aggaaggaaa tgtattgtgc gacgcgctca tctgcctgtt tcttggctgt agcggcatcc
     1021 ttcgcggcca cgttaacttc aatcgcaaga gtggcaatgt ctggcaccgc atccacactt
     1081 gccgtaccgg aggtgacaat atgcggtcca tccggcaatt cgttagcctg cgctgccatc
     1141 ccgctaatac ccattaatgc cgccagggcg ataactttga acttcacgtt agtctctccg
     1201 tgttaattga cttgcccaaa attcaggacc tctggaggca agcttagcgc ctctgtttta
     1261 tttttccatc agatagcgcc taactgaaca aggcttgtgc atgagcaata ccgtctctcg
     1321 ccagctgcaa ggctataaac cacatgacac atcccactac cagattgata atgcgctgtg
     1381 attttgccgt gcgcaggcgc ggtgccagcc aggctgcgag aatagccaga ccaaagaacc
     1441 acaggaaaga ggcgctaatt gtcccgagcg caaaccaacg ttttggttcg acgtcaagct
     1501 gcccgccaag gctgcccagt acaacaaaag tatccaggta aacatgcgga ttcagccagg
     1561 tcactgccag catggtggcg ataattttcc atctgccttg cttcaggact tcggcactag
     1621 ctaactcaat attactactc atcgctgttt taaaagcgcc aaaaccatac cacagcaaga
     1681 aggctacgcc gccccaggtg accagcgcca gcaaccacgg cgactgcatc aataacgcgc
     1741 tgccaccaaa aatcccggcg caaatcagga ccaaatcgct gatagcacaa agtaaggcaa
     1801 tcataatgtg gtactgacga cgaatgccct gattcatcac aaaagcattt tgtggaccga
     1861 gcggcagaat catagctgcc ccaagagcaa taccttgaaa gtaataagaa aacacgttgg
     1921 atattccgaa ttaagtatct gatgcagaga ctatatcgcg ggattgttat tagcggaaat
     1981 tgataatatt aattggcaat cagaataact aataagagag aggccagaga gtattatctg
     2041 gcctcagcgt tgattacgca gctttgtctt ctttcacccg cttaaagttc acatccattt
     2101 gcgggtacgg gaagctgata ccggcggcat caaattcacg tttaatacgc tccagcacat
     2161 cccagtacac gttttgcaga tcgccgctgt tgctccagac gcggaccacg aaattaatcg
     2221 acgatgcacc aagttcgttc agacgcacag tcatttcgcg atctttcaaa atgcgatctt
     2281 cagactggat aatattggtc aggatctgct taacctgatc gatatcggaa tcatacgcca
     2341 cgccaataat aaattcgtta cggcgaactg gctcgcgaga gaagttaata atatttccgg
     2401 caataatttt accgttcgga ataacgataa ttttaccgtc tgcagtacgc atggtggtgg
     2461 agaaaatctg cacactcagc acagtaccgg ctacgccgcc gagatcaaca tattctccgg
     2521 cacggaacgg gcggaacatg acaagtaaca cgccagcggc caggttagaa agtgacccct
     2581 gcaaagccag accaacagct aagcctgcgg caccgagtac agcaatgact gacgctgttt
     2641 gtacacccac gcgtcccagg gcagcgatta gcgtaaaggc gataataccg taacggacta
     2701 atgcagaaag aaaatcagca acagtggcat cgattttacg ggagatcatc aggcgattca
     2761 ccgcgttgga aatcatccgc gcgataatca aaccaacgat gatgatcgcg agtgccgcca
     2821 cgatgtttac tgcataactt agcagcagcg cctggttagc taccagccag cttcccgcgc
     2881 cgtttatgct atcgacaaca ttcaaatctt ccattcaata ttccttttca actgactcaa
     2941 aatggagaaa tacactccgc cgccttatga cgggcagtct gacaagggta aacaataaag
     3001 cataattttg ccaaatagat cacagatttc tcatctgaat caccttgctt ttggcgaaaa
     3061 aatccccgca gagcggggct cagacagata tcaatgtgcg ctttgtcatg ccggatgcgg
     3121 cgtgaacgcc ttatccggcc tacaaaaacc tgcaaattca atatattgca ggatacgtgt
     3181 aggcctgata agcgtagcgc atcaggcaat tttgcttttg gcgaaaaaaa gacccgcaga
     3241 gcgggccttg agataagcag aaaggaatat cttacagaac gtcgatcgcg ttcagttcct
     3301 ggaatgcttt ttccagacga gcgatcatcg aagtctgacc ggcacgcagc catacgcgcg
     3361 gatcgtagta tttcttgttc ggctgatctt cgcctttcgg gttacccaac tgcccctgca
     3421 gataagcttc gttcgctttg tagtagttca gaacgccttc ccaggttgcc cattgggtat
     3481 cggtatcgat gttcattttt actacgccgt agcttacgga gtctttgatt tcctgagcag
     3541 tagaaccgga accaccgtgg aatacgaagt tcaggctgtt gtgcggcagg ttgtgtttct
     3601 tggaaacata ttcctgagaa tcacgcagga tggtcggagt cagaaccacg ttacccggct
     3661 tgtaaacacc gtgtacgtta ccgaaggacg cagcgatggt gaaacgcggg ctgattttgc
     3721 tcagttcggt gtatgcgtaa tcaacgtctt ccggctgggt gtacagtgca gaagcgtcca
     3781 tgtggctgtt gtccacgccg tcttcttcac caccggtgca acccagttcg atttccagag
     3841 tcatgccgat tttggacatg cgctccaggt atttagagca gatttcgatg ttctcttgca
     3901 gagattcttc agacaggtcg atcatgtgag aagagaacag cggcttaccg gtagctgcga
     3961 agtgtttttc acccgcgtcc agcagaccgt cgatccacgg cagcagtttc ttcgcgcagt
     4021 ggtcagtgtg caggataacc ggaacaccat aatgttcagc catctggtga acgtgatgcg
     4081 caccagagat cgcgcccagg atagcagcac cctgcggaac gtcagatttc acgcctttac
     4141 cagcgataaa ggaagcacca ccgttggaga actgaacgat aaccggcgct ttaactttag
     4201 cagcggtttc cagtacggcg ttgatggagt cagtaccgac gcagtttact gctggcagtg
     4261 cgaagttgtt ttcttttgct acctggaaaa ctttctgtac gtcatcacca gtgattacgc
     4321 caggttttac gaaatcaaaa atcttagaca tgtctcttgt cctgtatcgt cgggccttat
     4381 actcatcata cttcaagttg catgtgctgc gtctgcgttc gctcacccca gtcacttact
     4441 tatgtaagct cctggggatt cactctcttg tcgccttcct gcaactcgaa ttatttagag
     4501 tatgaaaaaa tgcgtgctct aaaagcgcgc tgaaacaagg gcaggtttcc ctgccctgtg
     4561 attttttact tcttagcgcg ctcttcgagc atcgctactg caggcagtac tttaccttcc
     4621 acgaattcga ggaatgcgcc gccgccagtg gagatgtagg agattttgtc agcaatgccg
     4681 aacaggtcga ttgctgccag agtgtcgccg ccgccagcga tggagaacgc ttcgctgtct
     4741 gcgatagcgt tagccacgat ttcagtacct ttgcggaagt tcgggaattc gaacacgcca
     4801 accggaccgt tccacagaat ggttttcgca ttcttcagga tttcagccag ttcctgagcg
     4861 gaagcatcac cgatatccag gatctgctcg tcagctttca catcgttaac agatttcagg
     4921 gtagccgatg cagtttcaga gaactcggtt gctacgcgaa catcagacgg aaccgggatg
     4981 ttgcaggtgg tcagcagacg tttagcttcg tcaaccaggt cagcttcgta cagggattta
     5041 cccacatcgt ggccttgtgc cgcgataaag gtgttagcga taccaccacc aacaatcagc
     5101 tggtcagcga ttttagacag ggagtccaga acggtcagtt tggtagatac tttagaacca
     5161 ccaacgatag ccaccatcgg gcgagcaggt tctttcagtg ctttacccag cgcgtccagt
     5221 tcagctgcca acagcgggcc tgcgcacgca acatcagcga atttaccgat accgtgagta
     5281 gaagcctgcg cgcggtgagc agtaccgaat gcgtccatta cgaacacgtc acacagtgca
     5341 gcgtattttt tggacagggt ttcgtcgtct ttcttctcgc ctttgttgaa gcgaacgttt
     5401 tccagaacaa ccagttcacc ttcagcaacg tcaacgccgt cgaggtaatc tttaaccaga
     5461 cgaaccgggt tagacagttt gtctttcagg tagttaacaa ccggcagcag agagaattct
     5521 tcgttgtact cgccttcggt aggacgaccc aggtgggaag ttaccatcac tttcgcgcct
     5581 tgtttcaggg ccagttcgat ggtcggcaga gaagcacgga tacgcgcgtc gctggttact
     5641 ttcccgtctt ttactggtac gttcagatcc gcacggataa atacacgttt cccagcaaga
     5701 tccagatcgg tcatcttaat tacagacatg gtgaatcctc tcgttgattc taaaagtttt
     5761 gcagacgctg cttgcgtctt acctgaaagc aacagtagcc atagctaacg tcgtgtcgag
     5821 cattcggtta gcaaagcccc attcgttatc gcaccagacc aacgttttga tcaggtgtgc
     5881 gccactgacc cgggtttggg tgccatcgac aatggcactg tgcggatcgt ggttaaaatc
     5941 tacagagacc aacggcaatt ccgtatagtc aactatacca tgaaatgcac cttgtgctgc
     6001 tttttgcagc aacaggttga cttcattggc ttttacaggt ttcttcaccg tcacgcttaa
     6061 atcgattgcc gtcacattta tggttggcac acgtaccgca atcgcttcaa agcgatcgtt
     6121 aaattgcgga aaaaatcgtg tgataccggc ggccagttta gtatcgaccg gaatgatcga
     6181 ctggctggct gcccgggtgc gacgcaggtc aggatggtat gcatcaataa cctgttgatc
     6241 gtgcatggcg gagtgaattg tggtcacagt gccggactca ataccgtacg catcatctaa
     6301 caatttgatg acgggaatta tgcaattcgt ggtacaggaa gcgttagaaa cgatgcggtg
     6361 ttccgcacga agttgatcct gattgacgcc gtaaacaacg gtcgcgtcga gatcgttact
     6421 gccaggatgt gaaaagagca cttttttggc cccggcggca atatgcgctt cgccatgctc
     6481 gcgggagcca tatacgccgg tgcagtcgag gactacatca acgccaagtt cacgccaggg
     6541 gagcgattgc agtgaacgtt catgcaatac gcggatggcg tcatcaccaa caaaaagttg
     6601 atcgcgttcc tgtcgtactt cccatgcaaa acggccatgg ctggtgtcat atttcaacaa
     6661 atgcgccatg cccgcagcat ccgccagttc gttgattgcc accacggtaa tttccgcccg
     6721 gcgtccggat tcatacaaag cacgaaccac attacgcccg atgcgaccga agccatttat
     6781 cgctacgcgt acggtcatag atctcctgca aggttttccc tgagcaaatt tgccagacag
     6841 agtaatccag caaatcgtcc ggggaaacct tacctgtcgc aaactgcgac tgattggtta
     6901 attgtcgaac atttaatcga ctgaaacgct tcagctagga taagcgaaac gtggaataaa
     6961 aggaatgatt gtccagtcaa agaaaacaat tatctgactt acgtcacgtt ttcagccccg
     7021 cccctgtgga atttattcca ctttgcagaa tcacgaataa gcgtagcaaa atcttttcgt
     7081 tgtgccgtca caaaccctga cttatatcag aatatgactg atgcagcgtc gatattatcc
     7141 gcaatacgtc cgagtttcgg agtactccaa tgatgacatt gccctaccca tattcttccc
     7201 ccgcacgctt ttatgcactg cgcctcctcc ccgggcagga agttttttcc cagctacacg
     7261 cttttgcaca gcagcatcaa cttcatgccg cctggattgc aggctgtacc ggtagcctga
     7321 ctgacgtcgc cttgcgctat gccgggcaag aaggcaccac gctccttaac ggtacattcg
     7381 aagtgatctc actaaacggc acgctggaac agagtggtga gcatcttcat ctctgcgttt
     7441 ccgatccaca cggcacgatg cttggcggtc atatgatgcc cggctgtacc gtgcggacca
     7501 cgcttgaact ggtgatcggt agccttgagg aactggcgtt tagccgccaa ccttgcgcgc
     7561 tttctggtta cgacgagttg catatttccc ccgttaaata actactttca aagaggtttt
     7621 cttatggcgc gtagccactt ttccagtcag gcactggtgt tgatcgttat atctatcgcc
     7681 atcaatatga tcggcgggca actggccagc atggtcaaat tacctatatt tctcgactcc
     7741 atcggcacac ttattagcgc ggtactgctt ggtccggtga tcggaatgct gaccggctta
     7801 ctgaccaatt tactctgggg attgctgacc gacccgatcg ccgccgcatt tgcacccgtc
     7861 gcgatggtca tcggcctggt cgccggttgg ctggcgcgag cgggttggtt tcgcaccctg
     7921 ccaaaagtcg ttgtcagcgg cgtgattatt acgcttgcgg taacggtcgt cgctgtaccg
     7981 ctgcgtactg cgctgttcgg cggtgtcacc ggtagcggag ccgatttgtt cgttgcctgg
     8041 atgcactcga tggggcaaaa cctggtggag tcggtggcga ttacagtgat aggcgcgaac
     8101 ctggtcgata aaattcttac agcggtgatt gtctggcttt tactgcgcca gctgccgatt
     8161 cgcaccacgc gccattttcc ggcaatggct gccgtgcgct aaatgcatcc atttacctca
     8221 ttaacactgt gggcattggc ggcctgcacc acgcttatcc tgcccgcgca gaccattttg
     8281 ccgatctata gcgcggcgac ctttttttgt cttattgcgc taaaggccac gcggcggcgg
     8341 gcaaaatatg tcgcctggct gatgttctcg ctgggagccg ggctgtggct ggtacacggt
     8401 ggctggctaa cagaatggct aagcggcacg ccgcgttcgc cggagcgttg gacgcatgct
     8461 attacgctct ggttgcggat tctggcgatt gtttcaacct cacagttgtg gatgcagtac
     8521 gtccccgtgc aatgttttat ccgcgctctg tttgcttctc gcctgccgcc aggcgtcgct
     8581 tatctgtttg ccggaccgtt attggtcgtt gagcagttaa aacggcaatt agcgattatt
     8641 catgaagcgc agcgtgcccg cggcgtgccg ctggatgagg gctggtatca gcgtttacgt
     8701 gcgatgcccg cgttaattat tctgctaaca cacaatgcgc ttaacgatct ggctgtccgt
     8761 ggtgcggcgc tggatatgcg ggcatttcgg atccacaacc gacgaacaac gttgtgggca
     8821 ccagccgata gcaccctaca acgcgtagcg cgttacacca tgatcttgct gatgctaaca
     8881 gaattcggag cgtggatatg gttacgttag aacagttccg ttattgcccg acacactcaa
     8941 cgcatcctcc gttctgttat gactttcact atgtcaaacc ggggatggtc gccatctttg
     9001 gtgacaacgg cagtggcaaa agtacgctgg cacagctgat ggcgggctgg tatccggact
     9061 ttctgccagg agagataaca ggcacgggga cgctcctcgg tacgcccatt gggcatctcc
     9121 cgcttaatga acaatccgcc accatccagc tcgtccagca atcgccctat ttgcagctct
     9181 ccggctgtac ctttagcgtg gaagaagaag ttgcctttgg gccagaaaat ctgtgtcttg
     9241 ctgaaacaga gattatggcg cggattgatg ccgccctgac tctgaccgag tgccagccgt
     9301 tgcgccatcg tcacccggca acactctccg gtggagagac acaacgcgtg gtcatcgcct
     9361 gcgccatcgc gatgcagcca aagctcttaa ttcttgatga agcctttagt cgcctgacgc
     9421 cgcaggccag cgaaatgctg ttgcagcgct tacaacactg ggcgcttgaa cgtggttcgc
     9481 tgattattct ttttgaacgt catcgcacac ctttcctgaa ctactgccag caagcgtggc
     9541 aattgcaaaa cggagcgctc cagccactat gttaacgtta aatcagatat cttatcgctg
     9601 gcctagcgca gcaacggatt gtctttgcga catttctctg caactcaaac aaggtgaatg
     9661 gttggcgctg accggcgaca atggcgcggg aaaatcgacg ctgttacgag taatggcagg
     9721 tcttcttacg cccacagccg gtacggttat gctgcaacaa caggcgatga aaaacctgaa
     9781 aaaccgccag cgcgcggcaa aggttggcgt actgtttcag gaagcggaaa accaactttt
     9841 tcacagcacc gtcgctgacg agatcgcatt tggtttgaaa ctgcaaaaat gcccggcgga
     9901 tgaaatcact caacgtaccc atgctgccct gcaatgttgt cagttagcag acactgccaa
     9961 ctcgcacccg ttggatctgc atagtgcgca gcgacggatg gtcgccgtcg cctgcctgga
    10021 agcactctct ccgccactat tactgctgga cgagccgagt cgggattttg acgaaaattg
    10081 gttgagcgtg tttgaaagct ggctggagaa gtgccgccag cgaggcacga gcgtggtagc
    10141 aatcagtcat gacgccgcat ttacccggcg tcatttctct cgtgtggtgc gactggaaga
    10201 tggtgtaatc aggaacgtta atccaccaga cgatattcac ccgtagcggt catcattaag
    10261 gtcaaatttg caggcagact ctcttccagc acccggcgaa cattcggccc gtcggtacgg
    10321 tcataaaagg cttctgcatc tgccagcgat acccctcccg ccagcttgcg ccccaccaga
    10381 cgctcccgca acgcagtagc tggcgcgttg ataaagatcg agaaatcaca aaattgcgca
    10441 agctggcacc acttctcgtc gtccagcaac aaccagtttc cttcgacgat gacgagtggt
    10501 gcggtaacgt gcaacgcatc ttcaacagga tcatgctttt gtcgatcgta ctgcggccac
    10561 gtacaatccc cttccaccac ctggcgcaga ttttccgcca gtttcgcaac gtcaaatgtc
    10621 tctggtgcgc ctttgaaggg gcgcaattga tgcgcgtcca gccagctatt gtaatggtga
    10681 aaaccatcca tcgggagcgt ctgaatagcg ggcagctccg ggtcttgttg cacgagatat
    10741 tcccagaagg tggttagggt agatttaccc gtccccggcg gagcgcaaag aaaaaccacc
    10801 gtacgccgct gcggatttac cgtctgtaag gccgccagca tacgcagcaa cggtttatgc
    10861 acgcgttcta tctcttcatc atggtactgc gcttgcacgt tcagcccatt caccgttaat
    10921 tcaattttca cggcgttaat tcctttaagg tggtcgttac tgccagcgtc agcgccgttt
    10981 tgaccattcc gctaaaacgc gcc
//
LOCUS       DAEEKQ010000130        10943 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.131, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000130 DAEEKQ010000000
VERSION     DAEEKQ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10943)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10943)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10943
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.131"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..696)
                     /locus_tag="KLK57_003155"
     CDS             complement(<1..696)
                     /locus_tag="KLK57_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE0893456.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAG"
     gene            952..1107
                     /gene="rseD"
                     /locus_tag="KLK57_003156"
     CDS             952..1107
                     /gene="rseD"
                     /locus_tag="KLK57_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HBE0893457.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            1104..1679
                     /gene="rpoE"
                     /locus_tag="KLK57_003157"
     CDS             1104..1679
                     /gene="rpoE"
                     /locus_tag="KLK57_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HBE0893458.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            1712..2362
                     /gene="rseA"
                     /locus_tag="KLK57_003158"
     CDS             1712..2362
                     /gene="rseA"
                     /locus_tag="KLK57_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HBE0893459.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWHKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGLQTLGTQSQ"
     gene            2362..3318
                     /gene="rseB"
                     /locus_tag="KLK57_003159"
     CDS             2362..3318
                     /gene="rseB"
                     /locus_tag="KLK57_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HBE0893460.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVISFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            3315..3794
                     /gene="rseC"
                     /locus_tag="KLK57_003160"
     CDS             3315..3794
                     /gene="rseC"
                     /locus_tag="KLK57_003160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HBE0893461.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPDLVRFETSSEDASQ"
     gene            3992..5791
                     /gene="lepA"
                     /locus_tag="KLK57_003161"
     CDS             3992..5791
                     /gene="lepA"
                     /locus_tag="KLK57_003161"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HBE0893462.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGSLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            5807..6781
                     /gene="lepB"
                     /locus_tag="KLK57_003162"
     CDS             5807..6781
                     /gene="lepB"
                     /locus_tag="KLK57_003162"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HBE0893463.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKLGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            7054..7734
                     /gene="rnc"
                     /locus_tag="KLK57_003163"
     CDS             7054..7734
                     /gene="rnc"
                     /locus_tag="KLK57_003163"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HBE0893464.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            7731..8636
                     /gene="era"
                     /locus_tag="KLK57_003164"
     CDS             7731..8636
                     /gene="era"
                     /locus_tag="KLK57_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HBE0893465.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            8648..9376
                     /gene="recO"
                     /locus_tag="KLK57_003165"
     CDS             8648..9376
                     /gene="recO"
                     /locus_tag="KLK57_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HBE0893466.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            9388..10119
                     /gene="pdxJ"
                     /locus_tag="KLK57_003166"
     CDS             9388..10119
                     /gene="pdxJ"
                     /locus_tag="KLK57_003166"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HBE0893467.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKMRDACKRLTDAGIQISLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            10119..10499
                     /locus_tag="KLK57_003167"
     CDS             10119..10499
                     /locus_tag="KLK57_003167"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HBE0893468.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            10721..10801
                     /gene="shoB"
                     /locus_tag="KLK57_003168"
     CDS             10721..10801
                     /gene="shoB"
                     /locus_tag="KLK57_003168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HBE0893469.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
BASE COUNT         2761 a         2677 c         2904 g         2601 t
ORIGIN      
        1 gcccgcgcgc cacgccatag caatgccatc gccagaagaa atatccggat tggtggtgta
       61 ctgatacacc ttcgacgcac cgccggttgc cagtaccact gcttttgcgt ggcaggtttc
      121 caccgtttct ttatttcggt tccataccca cgcgcctaca acccgtcgcg tgcccggcag
      181 gccaattttg tcagagacaa tcagatcaac tgcgttgctg cgctccagca cacgaatatt
      241 cggatggttc tgcgccttgc tcaccagcgt ggattgtact tctctaccgg tggcgtcggc
      301 ggcatgaaga atacgacggt gactatgtcc accttcacgg gtcagatggt agctttcttc
      361 gccattcggt tgaacgtggg tatcaaacaa aaccccctgg tcaattaacc attgcacaca
      421 ggaacgagca ttgctggcga caaattcaac tgcatggcga tcgcaaatac cagccccggc
      481 aatcaatgtg tcttccacat gcgagtcaat gctgtcagtt tcatcaaaca cggcggcaat
      541 accgccctgg gcataaaatg ttgaaccttc cgttaccggg cctttactta gaacgatgac
      601 ctgatgctgg tcagccaggc gtagcgccag tgaaagtccg gctgcgccgc taccaataat
      661 caacacgtca catgaatgtt cagggagagt attcattttc tttgtttaat ttactaaaca
      721 ttgtttggtc agcatagcat catgttgtgc gaataaacac ctgctatttt aatatttgtt
      781 acagtggcta aacacgttga cgcagggcgg cgagaaaaag agaagttact ggctggtgga
      841 ggattaggtg gtgaaataaa aaggccgttg ggttactctt caggcagtta aatgggcatt
      901 tctacacaga taatgcgatg ttcagattct gtagacttat aatgatagat aatgatccgt
      961 ctacagcatg acaaacaaaa acagatgcgt tacggaactt tacaaaaacg agacactcta
     1021 accctttgct tgctcaaatt gcagctaatg gagtggcgtt tcgatagcgc gtggaaattt
     1081 ggtttgggga gactttacct cggatgagcg agcagttaac ggaccaggtc ctggttgaac
     1141 gggtccagaa gggagatcag aaagccttta acttactggt agtgcgctat cagcataaag
     1201 tggcgagtct ggtttcccgc tatgtgccgt cgggtgatgt tcccgatgtg gtacaagaag
     1261 cttttattaa agcctatcgt gcgctggatt cgttccgggg agatagcgct ttttatacat
     1321 ggctgtatcg gattgctgta aatacagcga aaaattacct ggttgctcag gggcgtcgtc
     1381 caccttccag tgatgtggat gccattgaag ctgaaaactt cgaaagtggc ggcgcgttga
     1441 aagaaatttc gaaccctgag aacttaatgt tgtcagaaga actgagacag atagttttcc
     1501 gaactattga gtccctcccg gaagatttgc gcatggcaat aaccttgcgg gagctggatg
     1561 gcctgagcta tgaagagata gccgctatca tggattgtcc ggtaggtacg gtgcgttcac
     1621 gtatcttccg agcgagggaa gctattgata acaaagttca accgcttatc aggcgttgac
     1681 gatagcggga tactggataa gggtattagg catgcagaaa gaacaacttt ccgctttaat
     1741 ggatggcgaa acgctggata gtgagctgct taacgaactg gctcataacc cagaaatgca
     1801 gaaaacctgg gaaagctatc acttaatccg tgattcaatg cggggtgata ctcccgaggt
     1861 gctccatttc gatatctctt cacgcgtgat ggccgccatt gaagaagagc cagtacgtca
     1921 accggcgaca ttgatcccgg aagcccagcc tgcgccgcat caatggcaca aaatgccatt
     1981 ctggcagaaa gtacgtccgt gggcggcaca gcttacccaa atgggcgtag cagcatgcgt
     2041 atcgcttgca gttatcgttg gcgtccagca ctataatgga caatctgaaa cgtcccagca
     2101 gcccgaaacg ccggtattta atacactgcc gatgatgggt aaagccagcc cggtaagcct
     2161 gggagtacct tctgaagcga ccgcaaacaa tggtcaacag cagcaggtac aggagcagcg
     2221 tcgtcgcatt aatgcaatgt tgcaggatta cgaactgcaa cgccgactcc actctgaaca
     2281 gcttcagttt gagcaggcgc aaacccagca agccgctgta caggtgccag gacttcaaac
     2341 tttaggaacg caatcgcagt aatgaagcaa ctttggtttg ccatgtcatt agtgacaggt
     2401 agcctgttat tctctgctaa cgcctcggcc actcccgcgt ccggggcgtt attacagcag
     2461 atgaacctgg ccagtcagtc actgaattac gagctgtcat tcatcagcat caataaacag
     2521 ggtgttgagt ctctgcgtta tcgacatgca cgcctcgata accgtcctct tgcacaattg
     2581 ttgcaaatgg atggcccgcg ccgggaagtg gtacagcgcg gcaatgaaat cagctatttt
     2641 gaaccgggac ttgaaccgtt cacgcttaat ggcgattaca ttgttgattc tctgccatcg
     2701 cttatctata ccgatttcaa acgcctttct ccttactacg actttatctc cgtcgggcgc
     2761 acgcgtattg ctgatcgtct ttgcgaagtc attcgcgtgg ttgcccgaga tggtacacgc
     2821 tacagctaca tcgtgtggat ggacactgaa tcgaaattac cgatgagggt tgatctcctt
     2881 gatcgcgatg gtgaaacgct ggaacaattt cgcgtgattt cttttaacgt caatcaggat
     2941 atcagcagca gtatgcagac gctggcgaag gcaaatttgc cgccgttgct ttctgttcct
     3001 gtaggtgaaa aagctaaatt cagctggacg ccaacctggt tgccacaggg ttttagcgaa
     3061 gtttccagta gtcgacgtcc gctaccgacg atggacaaca tgcctatcga atcacgtctc
     3121 tattccgacg gattattcag cttctcggta aacgttaacc gcgctacgcc atcgagcacc
     3181 gatcagatgt tgcgcaccgg acgcagaacc gtcagtacaa gcgtacgtga taacgccgaa
     3241 atcaccattg tcggtgaact gccgccgcaa acggcgaaac gcattgccga gaatattaag
     3301 ttcggggcag cgcaatgatc aaagagtggg ctaccgtcgt ctcctggcaa aacgggcagg
     3361 cgctggtcag ttgtgatgtt aaagcctcat gcagcagctg cgcttcacgc gccggttgcg
     3421 gtagccgcgt gttgaataaa cttggcccgc aaaccacgca taccattgtc gtaccctgtg
     3481 atgaaccgtt agtgccgggg caaaaagtgg aattggggat cgccgaaggc agcctgctta
     3541 gctccgcatt actggtttat atgtcgccgc tggtgggatt attccttatc gcttcgctat
     3601 ttcagctact ctttgcttct gacgttgcag cattatgtgg tgcgatcctt ggcggtattg
     3661 gcgggtttct gattgcgcgc ggttactcgc gtaagtttgc tgcccgggca gaatggcaac
     3721 cgatcatatt aagcgtggca ttgccgcctg accttgtgcg atttgaaaca tcttcggaag
     3781 acgcgagcca gtgatttcac tggctttgtt gcattttgca taacaaatgc gaggacgttt
     3841 ccagatacca caatccatac ggtatgaaca tttcctcgcc tcaatgttgt agtgtagaat
     3901 gcggcgtttc tattaataca gacgttaagc tcagaacagc gacttctaaa agcctggtta
     3961 accgggcatt aaggcacaat aatcatactt tatgaagaat atacgtaact tttcgatcat
     4021 agctcacatt gaccacggta aatcgacgct gtctgaccgt attatccaga tctgcggtgg
     4081 cctgtctgac cgtgaaatgg aggcgcaggt tctcgattcc atggatcttg agcgtgagcg
     4141 tggcattacc atcaaagcgc aaagcgtgac gctggactac aaagcgtctg acggcgaaac
     4201 ctatcagctt aactttatcg acaccccggg ccacgtagac ttctcctatg aagtttcccg
     4261 ttcgctggct gcctgtgaag gtgcattgct ggtggtcgac gccgggcagg gcgtggaagc
     4321 gcaaaccctg gcaaactgct acaccgccat ggaaatggat ctcgaagtgg tgccagtact
     4381 gaacaagatt gacctgccgg cagccgatcc tgaacgcgtg gcggaagaaa ttgaagatat
     4441 cgtcggcatc gacgccactg acgcggtgcg ctgttcagcg aaaaccggcg ttggcgtgca
     4501 ggacgttctc gaacgtctgg tgcgcgacat tccgccgccg gaaggcgatc cggaaggctc
     4561 gttgcaggca ctaattatcg actcatggtt cgacaactac ctgggcgttg tttcacttat
     4621 ccgtattaaa aacggcaccc tgcgtaaggg cgacaaagtg aaagtcatga gtaccgggca
     4681 gacctataac gccgaccgtc tgggcatctt cacgccgaaa caggttgacc gcactgaact
     4741 gaaatgtggc gaagtaggct ggctggtatg tgcgattaaa gatatccatg gcgctccagt
     4801 cggcgatacc ttaacgctgg cgcgtaatcc ggcagaaaag gcgctgcctg gctttaagaa
     4861 agtcaaaccg caggtatacg ccggtctgtt cccggtaagt tccgacgact atgaagcctt
     4921 ccgtgacgcg ctgggtaaac tcagcctgaa tgatgcctca ctgttctatg agccggaaag
     4981 ctccagcgcg ctgggctttg gtttccgctg cggcttcctt ggcctgctgc acatggagat
     5041 catccaggaa cgtctggaac gtgaatacga tctggatctg atcaccactg cgccgaccgt
     5101 agtgtatgaa gttgaaacca cgtcaaggga agttatctac gtcgacagcc catccaagct
     5161 gcctgcggta aataacattt acgaactgcg cgagccgatt gcagagtgtc acatgctgct
     5221 gccgcaggca tatctcggca acgttattac gctgtgcgta gaaaaacgcg gcgtgcagac
     5281 caatatggtt taccacggta atcaggtggc gctgacgtac gagatcccga tggcggaagt
     5341 ggtgctcgac ttcttcgatc gcctgaaatc tacctcgcgt ggttatgcgt ctctggatta
     5401 caacttcaaa cgcttccagg cgtccgacat ggtacgtgta gacgtattaa tcaacggtga
     5461 acgtgttgat gcgttggcgc tgatcaccca ccgtgataat tcgcaaaacc gcggtcgcga
     5521 gttggtggag aagatgaaag atctgatccc acgccagcag tttgatatcg ccattcaggc
     5581 agcgattggt acgcacatta ttgcgcgatc caccgtgaaa cagctgcgta aaaacgtact
     5641 ggctaaatgt tatggcggcg atatcagccg taagaaaaag ctgctgcaga agcagaaaga
     5701 aggtaagaaa cgcatgaagc agatcggtaa cgtcgagctg ccgcaggaag cgttcctcgc
     5761 cattctgcac gtcggcaaag acaacaaata acccttagga gttggcatgg cgaatatgtt
     5821 tgccctgatt ctggtgattg ccacactggt gacgggcatt ttatggtgcg tggataaatt
     5881 ctttttcgca cctaaacggc gggaacgtca ggcagcggcg caggcggctg ccggtgactc
     5941 actggataaa gcaacgttga aaaaggttgc accgaagctt ggctggctgg aaaccggagc
     6001 ttctgttttt ccggtgctgg ctatcgtatt gattgtacgt tcgtttattt atgaaccgtt
     6061 ccagatcccg tcaggttcga tgatgccgac tctgttaatc ggtgatttta ttctggtaga
     6121 gaagtttgct tatggcatta aagatcctat ctaccagaaa acgctgatcg aaaccggtca
     6181 tccgaaacgc ggcgatatcg tggtctttaa atatccggaa gatccaaagc ttgattacat
     6241 caagcgcgcg gtgggtttac cgggcgataa agtcacttac gatccggtct caaaagagct
     6301 gacgattcaa ccgggatgca gttccggcca ggcgtgtgaa aacgcgctgc cggtcaccta
     6361 ctcaaacgtg gaaccgagcg atttcgttca gaccttctca cgccgtaatg gtggggaagc
     6421 gaccagcgga ttctttgaag tgccgaaaaa cgaaaccaaa gaaaatggaa ttcgtctttc
     6481 cgagcgtaaa gagacactgg gtgatgtgac gcaccgaatt ctgacagtgc cgattgcgca
     6541 ggaccaggtg gggatgtatt accagcagcc agggcaacaa ctggcaacct ggattgttcc
     6601 gccgggacaa tacttcatga tgggcgacaa ccgcgacaac agcgcggaca gccgttactg
     6661 gggctttgtg cctgaagcga atctggtcgg tcgggcaacg gctatctgga tgagtttcga
     6721 taagcaagaa ggcgaatggc cgacaggtgt gcgcttaagt cgcattggcg gcatccatta
     6781 atagcccatc ttcgttcacg ttgtcgccgt tatggcgaca acgtgaatta tttatgagat
     6841 aaatctcccg tggctaacga catcccccgt cgttgtgtat agaatattcc cccgaagttt
     6901 aaggttggca cctccaggtt gccacggcac acgaaacagc gttggtcccc atataccggt
     6961 aaactgaaac tgcagcgaag cagttagcag agccatgtat atcaggtctg tttcgtgtgc
     7021 tgaattgttg acgcatttat ttattggtat cgcatgaacc ccatcgtaat taatcggctt
     7081 caacggaagc tgggctacac ttttaatcat caggaactgt tgcagcaggc attaactcat
     7141 cgtagtgcca gcagcaaaca taacgagcgt ttagaatttt taggcgactc tattctgagc
     7201 tacgttatcg ccaatgcgct ttatcaccgt ttccctcgtg tggatgaagg cgatatgagc
     7261 cggatgcgcg ccacgctggt ccgtggcaat acgctggcgg aactggcgcg cgaatttgag
     7321 ttaggcgagt gcttacgttt agggccaggt gaacttaaaa gcggtggatt tcgtcgtgag
     7381 tcaattctcg ccgataccgt cgaagcatta attggtggcg tattcctcga cagtgatatt
     7441 caaaccgtcg agaaattaat cctcaactgg tatcaaactc gtttggacga aattagccca
     7501 ggcgataaac aaaaagatcc gaaaacgcgc ttgcaagaat atttgcaggg tcgccatctg
     7561 ccgctgccga cttatctggt agtccaggta cgtggcgaag cgcacgatca ggaatttact
     7621 atccactgcc aggtcagcgg cctgagtgaa ccggtggttg gcacaggttc aagccgtcgt
     7681 aaggctgagc aggctgccgc cgaacaggcg ttgaaaaaac tggagctgga atgagcatcg
     7741 ataaaagtta ctgcggattt attgccatcg tcggacgtcc gaacgttggc aaatccacat
     7801 tgttgaacaa actgctgggg cagaagatct ccatcacctc ccgcaaagcg caaacaactc
     7861 gtcaccgcat tgtggggatc catactgaag gcgcgtatca ggcgatctac gttgataccc
     7921 ctggcctgca tatggaagaa aaacgcgcta ttaaccgcct gatgaacaaa gcggcgagca
     7981 gctctattgg cgacgttgag ctggtgattt ttgtcgttga aggcacccgc tggacgccgg
     8041 acgacgaaat ggtgctcaac aaactgcgcg atggcaaagc gccggtgatc ctcgcggtga
     8101 acaaagtgga taacgtgcag gaaaaagccg atctgctgcc gcacttacag ttcctggcaa
     8161 gtcagatgaa cttcctcgat atcgtgccga tctctgctga aaccgggctg aatgtcgaca
     8221 ctatcgcagc aatcgtgcgt aagcatctgc ctgaagcgac ccatcacttc ccggaagatt
     8281 acatcaccga tcgctcacag cgttttatgg cgtctgaaat catccgcgaa aaactgatgc
     8341 gtttcctcgg cgctgaactg ccgtactccg tgaccgtgga gatcgaacgt ttcgtctcta
     8401 acgaacgcgg tggttatgac atcaatggtt tgattctcgt tgagcgtgaa gggcagaaga
     8461 agatggttat tggcaacaaa ggggccaaga tcaaaaccat cgggattgaa gcgcgtaaag
     8521 acatgcagga aatgttcgaa gcgcctgtcc accttgaact gtgggtaaaa gtgaaatccg
     8581 gttgggccga cgacgaacgc gcactgcgca gtctcggtta cgttgacgat ctttaagagt
     8641 cactccgatg gaaggctggc agcgcgcatt tgtcctgcat agtcgcccgt ggagcgaaac
     8701 cagcctgatg ctggacgtct tcacggagga atcgggtcgc gtgcgtctgg ttgccaaagg
     8761 cgcacgctct aaacgctcta ctctgaaagg tgcattacag cctttcaccc ctctcttgct
     8821 acgtttcggc gggcgtggcg aagtcaaaac gctgcgcagt gctgaagctg tctcgctggc
     8881 gctgccatta agcggtatca cgctttacag cggtctgtac atcaacgaac ttctctcccg
     8941 cgtactggaa tacgagacgc gcttctctga actctttttc gattacttgc actgcattca
     9001 gtctcttgca ggtgccactg gtacgccaga acccgcgctg cgccgctttg aactggcact
     9061 gctcgggcat ctgggctatg gcgtcaattt tacccattgc gcgggtagcg gcgagccggt
     9121 agatgacacc atgacgtatc gttatcgcga agaaaaaggg tttatcgcaa gcgtcgttat
     9181 cgacaataaa acgttcaccg gaaggcagtt aaaagcgtta aacgcgcggg aatttcctga
     9241 cgcagacaca ctacgcgccg cgaaacgctt tacccgcatg gcgcttaagc cgtatcttgg
     9301 cggtaaacca ttaaagagca gggaactgtt ccggcagttt atgcctaagc gaacggtgaa
     9361 aacacattat gaatgatgag gattgtcatg gctgaattac tgttaggcgt caacattgac
     9421 catattgcaa cgctgcgtaa cgcacgcggt actgcttacc cggatccggt gcaggcggcg
     9481 tttattgccg agcaggcggg agcggacggc attaccgtgc atttacgtga agatcgccgc
     9541 cacattactg accgcgatgt gcgcatcctg cggcagacgc tggatacccg aatgaatctg
     9601 gagatggcag tgaccgaaga gatgctggcg atcgccgttg agacgaagcc acatttttgc
     9661 tgcctggtac cggaaaagcg tcaggaagta acaaccgaag gcggcctgga cgttgcaggg
     9721 cagcgtgaaa aaatgcgcga tgcctgcaaa cgtctgacag atgccgggat tcagatttct
     9781 ctctttatcg acgccgatga agagcagatc aaagctgcgg cagaggttgg cgcaccgttt
     9841 atcgagatcc acaccggttg ctatgctgat gccaaaactg acgccgaaca ggcgcaagag
     9901 ctggtgcgta tcgccaaagc cgcgaccttt gccgctagtc tcggtctgaa agttaacgcc
     9961 ggacacggtc tgacctatca caacgtgaaa gccattgccg ccatccctga gatgcatgaa
    10021 ctgaatatcg gtcatgccat tattggccgt gcagtgatga ccggactgaa agatgcggtg
    10081 gcagaaatga agcgtctgat gctggaagcg cgtggctaat ggcaatatta ggtttaggca
    10141 cggatatcgt agagatcgct cgcatagaag cagtgatcgc ccgatccggt gagcgcctgg
    10201 cacgccgcgt attaagcgat aacgaatggg caatctggaa aacgcaccac cagccggtgc
    10261 gttttctggc gaagcgtttt gctgtgaaag aagccgcagc aaaagcgttt ggcaccggga
    10321 tccgcaatgg tctggcgttt aatcaatttg aagtattcaa tgatgagctc ggcaaaccac
    10381 ggctacggct atggggtgag gcattaaaac tggcggaaaa gctcggcgtt gtaaatatgc
    10441 atgtaacgct ggcagatgaa cgccactatg cttgtgccac ggtaattatt gaaagttaaa
    10501 ataataccgt tctctttaaa aagtaaaacc cccgaactgg taaggaacgg gggttttact
    10561 ttttaacttt aattttgtgc agcatgcacc ctcaacaaca gcgattatgg atcagtgtac
    10621 ttatctttta caagcaactt gcaatcttta gcataaaaac tcgagccttt acgaagaaag
    10681 caatactgaa cggctgcatt ccttgtttaa ggaaaaacga atgactgatt gccgatacct
    10741 gattaaacgg gtcatcaaaa tcatcattgc tgttttacag ctgatccttc tgttcttata
    10801 acacaaggaa acgtacttaa ggtgcgtccg gtgaaccaat cggacgcgcc tttaataact
    10861 ataaataagt gtcttggcag atactatata aattaactta gtgaatgatt atgctaatgt
    10921 catcaattaa ataaatataa tgg
//
LOCUS       DAEEKQ010000131        10707 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.132, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000131 DAEEKQ010000000
VERSION     DAEEKQ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10707
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.132"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            319..894
                     /gene="ssuE"
                     /locus_tag="KLK57_003169"
     CDS             319..894
                     /gene="ssuE"
                     /locus_tag="KLK57_003169"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HBE0893470.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSAMKAQELLHGVFADDSQVIDYHHKPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNVHA"
     gene            887..1846
                     /gene="ssuA"
                     /locus_tag="KLK57_003170"
     CDS             887..1846
                     /gene="ssuA"
                     /locus_tag="KLK57_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HBE0893471.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLIYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDVNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VSAEVAALQQQTADLFYENRLVSKKVDIRQRIWQPTHLEGKQL"
     gene            1843..2990
                     /gene="ssuD"
                     /locus_tag="KLK57_003171"
                     /pseudo
     CDS             1843..2990
                     /gene="ssuD"
                     /locus_tag="KLK57_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
     gene            3002..3793
                     /gene="ssuC"
                     /locus_tag="KLK57_003172"
     CDS             3002..3793
                     /gene="ssuC"
                     /locus_tag="KLK57_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HBE0893472.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3790..4557
                     /gene="ssuB"
                     /locus_tag="KLK57_003173"
     CDS             3790..4557
                     /gene="ssuB"
                     /locus_tag="KLK57_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HBE0893473.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4764..7376)
                     /gene="pepN"
                     /locus_tag="KLK57_003174"
     CDS             complement(4764..7376)
                     /gene="pepN"
                     /locus_tag="KLK57_003174"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HBE0893474.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPEHFTLKIVNEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKTKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLEEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7642..8844
                     /gene="pncB"
                     /locus_tag="KLK57_003175"
     CDS             7642..8844
                     /gene="pncB"
                     /locus_tag="KLK57_003175"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HBE0893475.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9016..10416
                     /gene="asnS"
                     /locus_tag="KLK57_003176"
     CDS             9016..10416
                     /gene="asnS"
                     /locus_tag="KLK57_003176"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HBE0893476.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2452 a         2731 c         2853 g         2671 t
ORIGIN      
        1 ctttaaatga cattagactt aataataaca ataattgacc gccatttaaa tcaagcatac
       61 taaatccaat aagtgacaaa tctttaattt atagatttca tgaatattta aatattaact
      121 gaacgagtaa ttcattgatt caacatctaa tgactatcca acaaataaat atgcgtgata
      181 tataaagccc tttctttagt ttattttcag aaaaagatac acaaaattta tatttggcat
      241 tttcttgtct ctccgacaga ctgaccgaat tgttatcaat gttaacaaaa aaagaacaat
      301 tggttataag gagagagtat gcgtgtcatc accctggcgg gtagtcctcg ctttccttct
      361 cgctccagct ccttgctgga atatgcgcgg gaaaaactaa atggcctgga tgtagaggtt
      421 tatcactgga atctgcaaaa cttcgccccg gaagatctgc tttacgctcg tttcgatagt
      481 ccggcactca agaccttcac cgaacagctg caacaggctg atggactgat agtcgctaca
      541 cctgtgtata aagccgccta ttccggcgcg ctgaaaaccc tgctcgacct gctgccagag
      601 cgcgctttgc aaggcaaagt ggtgctaccg ctggcgacgg gcggtacggt ggctcatctg
      661 ctggcggtcg attatgccct taagccagtt ttaagtgcga tgaaagccca ggagctcctg
      721 cacggcgtgt ttgccgatga ctcacaagtt attgattacc atcacaaacc ccagttcacg
      781 ccaaatctgc aaacccgtct tgataccgcg ctagaaactt tctggcaggc attgcaccgc
      841 cgcgatgttc aggttcctga ccttctgtct ctgcgaggta atgtccatgc gtaacatcat
      901 taaactggcg ctggcgggat tgcttagcgt ctctactttt gcggttgctg cagaatcctc
      961 gcctgaagcg ttacgtatag gctatcagaa aggcagtatt ggtatggtat tggcaaaaag
     1021 ccaccagtta ctggaaaaac gctatccgca atcaaaaatc tcctgggtgg agttccccgc
     1081 gggtccgcaa atgttggaag cgttaaacgt tggcagtatt gatctcggca gtaccgggga
     1141 tattccgcca atctttgccc aggctgccgg ggctgatttg atatatgtgg gcgtcgagcc
     1201 accgaagccc aaagccgaag tgattctggt ggcagaaaac agcccgatca aaaccgtagc
     1261 cgatcttaaa ggtcacaaag ttgcctttca gaaaggttcc agttcacaca accttttact
     1321 gcgtgcactg cgtcaggccg gacttaagtt tactgacatc cagcccactt atctgacgcc
     1381 agctgatgcc cgcgccgcgt tccagcaagg taacgttgac gcctgggcta tctgggatcc
     1441 ctactactcc gctgcgttat tacagggcgg cgtgcgggtg ctgaaagacg gcaccgatgt
     1501 caatcaaact ggatcgtttt atctggcagc tcgcccctat gcagaaaaaa acggcgcttt
     1561 tattcagggc gtactggcaa cctttagtga ggccgatgcg ttaacccgca gccagcgcga
     1621 gcaaagcatc gctttactgg caaaaacgat gggcttaccg gcaccggtga ttgcctcgta
     1681 tctggatcat cgtcctccca ccaccatcaa accggtgagc gctgaagttg ccgccttaca
     1741 gcagcaaacg gcagatctgt tttatgaaaa ccgtctggtg tcgaaaaaag ttgatattcg
     1801 ccagcgcatc tggcagccca ctcacctgga aggaaaacaa ttatgagtct gaatatgttc
     1861 tggtttttac cgacccatgg tgacgggcat tatcttggaa cggaagaagg ttcacgcccg
     1921 gttgatcacg gctatctgca acaaattgcg caagcggcgg atcgtctggg ctataccggt
     1981 gtgctgatcc caacggggcg atcgtgtgaa gatgcgtggc tggtggcggc gtcgatgatc
     2041 ccggtgacgc agcggctgaa gtttcttgtc gccctgcgtc ccagcgtaac ctcacctacc
     2101 gttgccgctc gccaggccgc cacgcttgac cgtctctcaa atggtcgtgc gttgtttaat
     2161 ctggtcacag gcagcgatcc acaagagttg gcaggcgacg gcgtgttcct tgatcatagc
     2221 gagcgctacg aagcctcggc ggaatttacc caggtctggc ggggcgctta ttgctggggg
     2281 aaagcgtcga tttcaacggt aaacatattc atgtgcgggg agcaaaactg ctcttcccgc
     2341 cgattcaaca gccgtatcct ccgctttact ttggcggatc gtcagatgtc gcccaggagc
     2401 tggcggcaga acaagttgat ctctacctca cctggggcga accgccagag ctggtaaaag
     2461 agaagatcga acaagtgcgg gcgaaagctg ccgcgcatgg acgcaaaatt cgtttcggta
     2521 ttcgtctgca tgtgattgtt cgtgaaacta acgacgaagc gtggcaggcc gccgagcggt
     2581 taatctcgca tcttgatgat gaaactatcg ccaaagcaca ggccgcattc gcccggacgg
     2641 attccgtagg acaacagcga atggcggcgc tacataacgg caagcgcgac aatctggaga
     2701 tcagccccaa tttatgggcg ggcgttggct tagtgcgcgg cggtgccgga acggcgctgg
     2761 tgggcgatgg tcctacggtc gccgcgcgaa tcaacgaata cgccgcgctt ggcatcgaca
     2821 gctttgtgct ttcgggctac ccgcatctgg aagaagcgta tcgggttggc gagttgctgt
     2881 tcccacatct ggatgtcgcc attccggaaa ttccccagcc gcagccgctg aatccgcaag
     2941 gcgaagcggt ggcgaatgat tttatccccc gtaaagtcgc gcaaagctaa ggaacacgat
     3001 gatggcaacg ccagtgaaga agtggttatt gcgcgttgcc ccctggtttt taccggtggg
     3061 catcgtggcg gtgtggcaac tggcctcctc ggttggctgg ctgtcgacgc gtattttgcc
     3121 ttcaccggaa ggagtggtga cggcgttctg gacgctctcc gccagcggcg aattgtggca
     3181 gcatctggcg atcagttcct ggcgggcgct gattggcttt tcaattggcg gatcgctggg
     3241 cttgattctg ggactgatta gcgggctgtc acgctgggga gaacggctgc tggatacctc
     3301 aattcagatg ttgcgcaacg taccgcatct ggcgctgatc ccgctggtga ttttgtggtt
     3361 tggcattgat gaatccgcaa aaatctttct ggtggcgctc ggcacgctat tccccattta
     3421 tatcaacacc tggcatggca tccgtaatat cgatcgcggt ctggtggaaa tggcgcgtag
     3481 ctatggatta tccggcatac cgctgtttat ccatgtgatc ctgcctggtg ccctgccctc
     3541 aattatggtc ggcgtgcgtt ttgcgttggg cctgatgtgg ctgacgctga ttgttgccga
     3601 aaccatttct gccaattcag gcattggtta tctggcgatg aatgcgcggg agtttttgca
     3661 aacggacgtg gtggtagtcg ctattattct ttacgccctg ctcggcaaac ttgctgacgt
     3721 cagcgcgcag ctgttagagc gcctctggtt gcgctggaac ccggcttatc atttgaagga
     3781 ggccacggta tgaatactgc tcgtctgaac cagggcacac cattgttgct caatgcagta
     3841 agcaaacatt acgcggaaaa tatcgtcctg aaccaactgg atttacatat tccggcaggt
     3901 cagtttgtgg cggtggtggg ccgcagcggt ggtggcaaaa gtaccctgct gcgcctgctg
     3961 gcaggtctgg aaacgccaac cgcaggcgat gtgttagcgg gcaccacacc gctggctgag
     4021 attcaggaag acacgcgaat gatgtttcag gatgcgcgtc tgctgccgtg gaaatcggtg
     4081 attgataacg ttgggttagg ccttaaaggc cagtggcgcg atgccgcgcg tcgggcgttg
     4141 gctgcagtag gactggagaa tcgcgccggg gaatggcctg ccgcactttc tggcgggcag
     4201 aaacagcgtg tggcgctggc aagagcgttg attcatcgac cgggtttatt gttgcttgat
     4261 gaaccgctcg gggcactgga cgccttaacg cgactcgaga tgcaggattt gattgtgtca
     4321 ctttggcagc agcacggctt taccgtactg ttggtgacgc atgatgtgag cgaagcggta
     4381 gcaatggctg accgggtgtt gttaattgaa gagggaaaaa ttggtctgga tttgacagtg
     4441 gatatccccc gtccgcgtcg attagggtcg gtgcgattag cggagctgga agcggaagta
     4501 ttgcaacggg taatgcggcg tgggcattct gaacaactga ttcggcgtca tggttaatat
     4561 gccggatgtg gcgcacaaaa ccagcatttt caacggactt gtaggcctga taagacacgt
     4621 caagcatcgc atcaggcagt tgggcacggc tgccggatgc ggcgtgaacg ccttatccag
     4681 cctacaaaac cagcaatttc aacggagttg taggcctgat aagacgcgtc aagcgtcgca
     4741 tcaggcattt tggctcaaaa gtatcaagcc agtgctttgg tgatcttctc gtacagatcg
     4801 ccagagagat tttccagccc tttcaactgt tccagcgccg cgcgcatttt ctcctgacgt
     4861 ttggcatcat aacgtttcag gcgaatcagc ggttcaatca gacgtgaagc tacctgcggg
     4921 ttacggctgt tgaggtcggt aagcatttcc tccaggaact ggtaaccgct gccatcttcg
     4981 gcatggaacg ctgccgggtt gctgcccgca aacgcaccaa tcaacgaacg aatacggttc
     5041 gggttgctca tggtaaatga gcgatgctgc aacagaccac gcaccgtctc cagcacattc
     5101 gccgccgggc tggtggcttg caggataaac catttatcca tcaccagacc gtcctgatgc
     5161 cacttatcgt cgtactcctg catcagcgcg tcacggcaag gcagctgtgc agcaaccgcc
     5221 gcagaaagcg ctgccagcgc atcggtcata ttgtttgctt catggtattg cttgctcacc
     5281 agcacgtcag ccagatgcgt ttcaccaaaa gcaaggaagc gcaggcaggc atttcgcaga
     5341 gtgcgttttg caatatcttc atgctcaaca cggtactcgc tctggtaatt cgcgttgtaa
     5401 atagccagca gctcatccgc cagttcagtc gccagagtac gagtgagtgc ttcgcgtact
     5461 tcggcaatag caatcgggtc gatgatatcg aacagttcag ccatttcatt gacagaaggc
     5521 agcgtcagga tttctgccgc cagcgccgga tcaatcttct catcgagcag caccgcgcgg
     5581 aaagcgtcag ccacatgtac cggcagagac agcggctgcc cttgctgatg acgggcgacg
     5641 ttcagcttga tgtaggttgc cagcaaactt tgcgccgcat cccaacggga gaaatcatta
     5701 cgcgcatgac gcatcaggaa ggtcagttgc tgatcactcc acttatattc cagtttcact
     5761 ggcgcagaga attcgcacag cagcgcaggc accggctgga agtagacatt atcaaaaaca
     5821 aaggtctgtt ccgcctgggt gacgttcagc acggaattca ccggatgacc gcctttctgc
     5881 aacgggatca ctttgccttc gttgtcatac aattcgatgg caaacggaat atgcagcggc
     5941 tgtttttctg cctgatccgg cgtggctggc gtgcgctggc tgatggtcag ggtgtactgc
     6001 tcagtttccg gattgtagtc gtctttgacg gtcacaatcg gcgtaccgga ctggctgtac
     6061 caacggcgga aatgggagag atcaacattc gacgcatctt ccatcgcctg cacaaagtcg
     6121 tcacaggtcg ctgcgctacc atcatgacgc tcgaaataga gttgcatccc tttctggaag
     6181 ttttcttcgc ccagcagggt gtggatcata cggatcactt ccgcgccctt ctcgtaaacg
     6241 gtcagggtgt agaagttgtt catctcaatg accatgtccg ggcggattgg gtgcgccatc
     6301 gggctggcgt cttccgcaaa ctgcaatccg cgcatggtgc gcacattatt gatgcggtta
     6361 actgcgcggg aaccaaggtc agagctgaat tcctgatcgc ggaagacggt taaaccttct
     6421 ttcaggctga gctggaacca gtcgcggcag gtcactcggt taccggtcca gttatggaaa
     6481 tattcatggc cgataacgcg ttcaatatcg aggtaatctt tgtcggtggc ggtgtcggtg
     6541 cgggccagca catatttgga gttaaagata ttcagaccct tattctccat tgcgcccata
     6601 ttgaagaaat ccaccgcgac gatcatatag atgtcgaggt catactccag gccgaagcgt
     6661 tcttcatccc atttcattga gtttttcagc gaggtcatcg cccacggcgc gcgatcaagg
     6721 ttgccgcgat cgacgtacag ctccagcgcc acttcgcgac cagaacgcgt ggtaaaggtg
     6781 tcgcgcagta catcaaagtc gcctgccacc agcgcaaaca ggtagcacgg tttcgggaac
     6841 gggtcctgcc actgtaccca atggcgtccg ttttccagtt caccttgcgc aacgcggtta
     6901 ccgttggaaa gcaggaaggg atatttggtt ttatcggcaa taattttggt ggtaaaacgc
     6961 gccagcacgt ccgggcggtc gagataatac gtaatatggc ggaaaccttc ggcttcacac
     7021 tgggtgcaaa gcgcatcgcc tgactgataa agcccttcca gcgcggtatt cgccgccggg
     7081 cttatttcat taacgatctt aagcgtaaaa tgctccggca aattactgat gaccagtgcg
     7141 ccctcttctt ctttccaggc ggtccacggc tcatcattaa tatgaacaga aaccagtttg
     7201 aggtcttcgc cgttgagacg aagtggagca tctgatgcac catgacggac agcctggctg
     7261 accgcggtaa cgaccgtctt ttgcgcgtcg aggtcaaagg tcaagtcaat atcagtaatc
     7321 tggtaatctg gcgcacgata atcgtgacgg tatttggctt gtggctgttg agtcataaat
     7381 aacctttagc atcttttata gagtctggtg ttcagtctat tcctgttgcg taaatcgcgc
     7441 tatgcagaat cttcatcttt tcaggtacaa acgcctttat tgctacattt ttataacata
     7501 caccgcgtaa tgccatcgac cagaaaggtg gcatatggtg tgatcggggt tcaataaatt
     7561 gcgaaacaag gtatactcca gcagttcctg aagatgttta ttgtactaaa cgctcctgta
     7621 cgaggacgct actgcgcacc tatgacacaa ttcgcttctc ctgttctgca ctcgttgctg
     7681 gatacagatg cttataagtt gcatatgcag caagccgtgt ttcatcacta ttacgatgtg
     7741 catgtcgcgg cggagtttcg ttgccgaggt gacgatctgc tgggtattta tgccgatgct
     7801 attcgtgaac agattcaggc gatgcagcac ctgcgcctgc aggatgatga atatcagtgg
     7861 ctctctgccc tgcctttctt taaggccgac tatcttaact ggttacgcga gttccgcttt
     7921 aacccggaac aagtcaccgt gtccaacgat aatggcaagc tggatattcg cttaagcggt
     7981 ccgtggcgtg aagtcatcct ctgggaagtt cctttgctgg cggttatcag tgaaatggta
     8041 catcgctatc gctcaccgca ggccgacgtt gcgcaagccc tcgacacgct ggaaagcaaa
     8101 ttagtcgact tctcggcgtt aaccgccggt cttgatatgt cgcgcttcca tctgatggat
     8161 tttggcaccc gtcgccgttt ttctcgcgaa gtacaagaaa ccatcgttaa gcgtctgcaa
     8221 caggaatcct ggttcgtggg caccagcaac tacgatctgg cgcgtcggct ttccctcacg
     8281 ccgatgggaa cacaggcaca cgaatggttc caggcacatc agcaaatcag cccggatcta
     8341 gccaacagcc agcgagctgc acttgctgcc tggctggaag agtatcccga ccaacttggc
     8401 attgcattaa ccgactgcat cactatggat gctttcctgc gtgatttcgg tgtcgagttc
     8461 gctagtcggt atcagggcct gcgtcatgac tctggcgacc cggttgaatg gggtgaaaaa
     8521 gccattgcac attatgaaaa gctgggaatt gatccacaga gtaaaacgct ggttttctct
     8581 gacaatctgg atttacgcaa agcggttgag ctataccgcc acttctcttc ccgcgtgcaa
     8641 ttaagttttg gtattgggac tcgcctgacc tgcgatatcc cccaggtaaa acccctgaat
     8701 attgtgatta agttggtaga gtgtaacggt aaaccggtgg cgaaactttc tgacagccct
     8761 ggcaaaacta tctgccatga taaagcgttt gttcgggcgc tgcgcaaagc gttcgacctt
     8821 ccgcatatta aaaaagccag ttaatatcat cagggagcta atcggctccc tttttttacc
     8881 tttaattccg aaatctttcg ctgcatttgc gaattctgct tgtctgattg cagatgccag
     8941 gtaacatagg tatccccccc ccatttacgg gtggacaagt gtttattttt tccgactatt
     9001 aacagagaga atattatgag cgttgtgcct gtagccgacg tactccaggg ccgcgtagcc
     9061 gttgacagcg aagtcaccgt acgcggatgg gtacgtaccc gccgagattc aaaagctggc
     9121 atctccttcc tcgccgttta tgacggttcc tgctttgatc ctgtacaggc tgtcatcaat
     9181 aattctctgc ccaattacaa tgaagacgtc ctgcgtctga ccaccggctg ctcggtcatt
     9241 gtgacgggta aagtcgtggc atcgccgggc caggggcaac aatttgaaat tcaggccagc
     9301 aaggttgaag ttgctggttg ggttgaagat ccagacactt acccgatggc ggcaaaacgc
     9361 cacagcattg agtatctgcg tgaagtcgct cacctgcgtc cgcgcacaaa cctgattggt
     9421 gccgtcgcgc gcgttcgcca tacgctggcg caggcgctgc atcgcttctt taacgagcag
     9481 ggattcttct gggtttcaac gccactgatt accgcatctg ataccgaagg tgcaggcgaa
     9541 atgttccgcg tttctacgct ggatctggaa aacctgccgc gtaacgatca gggcaaagtg
     9601 gatttcgaca aagacttctt tggtaaagag tctttcctga ccgtatctgg ccagttgaac
     9661 ggcgaaacct acgcttgcgc attatccaaa atttatacct tcggcccgac tttccgtgct
     9721 gaaaactcca acaccagccg tcacctggcg gaattctgga tgctggagcc ggaagtggcg
     9781 tttgctaacc tgaacgatat cgcgggtctg gctgaagcca tgctgaaata tgtcttcaaa
     9841 gcggttctcg aagaacgcgc agacgacatg aaattcttcg ctgaacgcgt agataaagat
     9901 gccgtttcac gtctggaacg cttcattgag gccgatttcg cgcaggtgga ttacaccgac
     9961 gcagtgacca ttctcgaaaa ctgcggcagg aagtttgaaa acccggttta ctggggcgtc
    10021 gatctctctt ctgagcatga gcgttatctg gcggaagaac actttaaagc accggtagtg
    10081 gttaaaaact atccgaaaga tattaaagcg ttctatatgc gcctcaacga agacggtaaa
    10141 accgttgcgg ctatggacgt tctggctccg ggcatcggtg agatcattgg tggctcccag
    10201 cgtgaagaac gtctggacgt tctggacgag cgtatgctgg aaatgggcct gaataaagaa
    10261 gattactggt ggtatcgcga tctgcgtcgc tacggtactg ttccgcattc aggtttcggt
    10321 cttggttttg aacgcctgat tgcttacgta actggcgtgc aaaacgtgcg tgatgtgatt
    10381 ccgttcccac gtactccgcg caacgccagc ttctgatttt cttcctcaaa gagccagcgc
    10441 ctgctggctc ttttttatcc tttctgtcaa gttatctgtt tgttaagtca agcaatctat
    10501 ttgcaacccc gccataacga tcatcctgtt acggaatatt acattgcaac atttacgcac
    10561 aaaaaataac ccgcattctt attgcggatt agttttttct tagctgatag cacaattttc
    10621 atactatttt ttggcattct ggatgtctga aagaggattt tgtgccaggt cgataaagtt
    10681 tccatcagaa acaaaatttc cgtttag
//
LOCUS       DAEEKQ010000132        10692 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.133, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000132 DAEEKQ010000000
VERSION     DAEEKQ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10692
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.133"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1543)
                     /locus_tag="KLK57_003177"
     CDS             complement(<1..1543)
                     /locus_tag="KLK57_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000482663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HBE0893477.1"
                     /translation="MFNLPGWLFTVIALVLTLFVIGLIFARLYRRASAEQAFVRTGLG
                     GQKVVMSGGAIVMPIFHETIPVNMNTLKLEVSRAAAESLITRDRMRVDVAVAFFLRVK
                     PSAEGISTAAQTLGQRTLTPEDLRSLVEDKFVDALRATAARMSMQDLQDARENFVQGV
                     QNTVAEDLSKNGLELESVSLTSFNQTARVHFNPDNAFDAEGLTLLTQETERRRRERNE
                     VEQDVEVAIREKNRDALSRRLEIEQQEAFMTLEQQQRVKTRTAEQSASIAAIEAERRR
                     EAESARILAERKIEEAEIERQQIVRTRQVEAEREVAIREIEQQQATEIASQARAIAVA
                     AKSEEQSQAEARASKALAEAVQAQQDVETTRQTAEADRTKQVALIAAAQEAETKAVEL
                     TVRAQAEKEAAELQAAAIIELAEATRQKGLAEAEAQRALNDAINVLSDEQTSLRFRLA
                     LLDALPAVIEKSTEPMKSIDGIKIIQVDGLNGGVTRGKTATTDISGGNLAEQAVTAAL
                     AYRTQA"
     gene            complement(1570..2190)
                     /locus_tag="KLK57_003178"
     CDS             complement(1570..2190)
                     /locus_tag="KLK57_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HBE0893478.1"
                     /translation="MTLTLLTAYNAPYLAALTFVLLTGIAELIALLCGHSLSSAMDTP
                     DLPEGLTGEALDWLNIGRIPLLIVLCMLAGFFGISGILLQGLIIHLLQAPAPNILLAP
                     LCLLLTCPLVHRTGRLIAPWLPRDETTALTEEAFIGCMATITGYHAVAGSPCEGRFTD
                     RFGQVHYLLLEPEAGKEFRRGDKVIIVCRLSGSRWLGELNPWPDVL"
     gene            complement(2457..3119)
                     /locus_tag="KLK57_003179"
     CDS             complement(2457..3119)
                     /locus_tag="KLK57_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="traC"
                     /protein_id="HBE0893479.1"
                     /translation="MTILSQENPVILLLENMLEALSTAPDNINNERRRRRYLLNWLDT
                     ARQMREFRGMAEEFTTLRKLLATNSDIPVANTLKSLLESGNNAALYDLYRFRSALATC
                     RSLGWQIGTCAWNDQLLSETLSRAQTGKRHILQLNSTGDTFSTTGRMIRPVAFSLIHP
                     ATINTSEVEEIFRDEGFMLAHGRECSLNGSGRNMLSRILHVGHSGLAEEEWGMDNSLL
                     LH"
     gene            complement(3260..3901)
                     /locus_tag="KLK57_003180"
     CDS             complement(3260..3901)
                     /locus_tag="KLK57_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusG"
                     /protein_id="HBE0893480.1"
                     /translation="MCLSELQARLNVGQPLFPTHALCIRRSQQHRSPPAGIDFRGAFR
                     ILNISELHQRNWYLAQYIPAGKNREHLFSWLSEQHVLPWTPLILKKVRRTDKVCGYRR
                     HIHAVFPGYFFLKADPESHSFTHLRRHSAFLDFVKMAGEIKTVREDIVQSLMKVYPDP
                     ALNPAAREELDAASTLWLTKARYQYLLRLDAQPLPESRIALLLHLVSDDGALT"
     gene            4603..5481
                     /gene="repA"
                     /locus_tag="KLK57_003181"
     CDS             4603..5481
                     /gene="repA"
                     /locus_tag="KLK57_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBE0893481.1"
                     /translation="MAALNQTSFTESTYRQVKNPNPVFTPREDAGTLKFCEKLMEKAV
                     GFTSRFDFAAHVAYARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTL
                     AIECGLATESEAGKLSITRATRALKFLSELGLITYQTEYDPTIGCNIPTDITFTPALF
                     DSLDISEEAVASARRSRVEWENRLRKKQGMDALGMDELIARAWRFVRERFRSYQAELK
                     SHGMKRARARRDAGRTRQDIVTLVKRQLTREIAEGRFRGSLEAVKREIDRRVKERMIM
                     SRNNNYTRLATASPLV"
     gene            6383..6652
                     /locus_tag="KLK57_003182"
     CDS             6383..6652
                     /locus_tag="KLK57_003182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893482.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVSDK"
     gene            6649..6882
                     /locus_tag="KLK57_003183"
     CDS             6649..6882
                     /locus_tag="KLK57_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE0893483.1"
                     /translation="MMEIFWIMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHGGTAEFGKNRTLS"
     gene            complement(7030..8607)
                     /locus_tag="KLK57_003184"
     CDS             complement(7030..8607)
                     /locus_tag="KLK57_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing methyl-accepting
                     chemotaxis protein"
                     /protein_id="HBE0893484.1"
                     /translation="MRQNLPVTGRNLELPKDANILSTTSPQSHITYVNPDFIKISGFT
                     EEELLGQPHNIVRHPDMPPAAFEHMWSTLKSGRSWMGLVKNRCKNGDHYWVSAYVTPI
                     AKNGSIVEYQSVRTKPEPEQVLAAEKLYAQLRSGKAARPKLAASFSVKILLLIWGSII
                     SSAMAAGMLTDTSISSLLLATLMSGSLSSVSVLAILSPLGRLVERAKNISNNPLSQSL
                     YTGRTDEFGQIEFALRMMQAETGAIVGRIGDASNRLSEHTRGLLKDIESSNVLTVEQQ
                     AETDQIATAVNQMVASIQEVASNAQHAADAAGRADTETASGQRLVAHTSQSITALEGE
                     IRQATQVIHELEGQSNEISKVLDVIRGIAEQTNLLALNAAIEAARAGEQGRGFAVVAD
                     EVRSLAARTQQSTTDIQSMISALQERAQSAVTVMEQSSRQAHTSVAHAEEAATALDGI
                     GQRVNEITDMNAQIATAVEQQGAVSEDINRSIINIRDAADTNVQTGQNNLQSAKSVAQ
                     LTSALSELAKQFWEKRG"
     gene            8917..9476
                     /locus_tag="KLK57_003185"
                     /pseudo
     CDS             8917..9476
                     /locus_tag="KLK57_003185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005220837.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            9480..>10692
                     /locus_tag="KLK57_003186"
     CDS             9480..>10692
                     /locus_tag="KLK57_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs1 family transposase"
                     /protein_id="HBE0893485.1"
                     /translation="MPRRLILSATERDTLLALPESQDDLIRYYTFNDSDLSLIRQRRG
                     DANRLGFAVQLCLLRYPGYALGTDSELPEPVILWVAKQVQAEPASWAKYGERDVTRRE
                     HAQELRTYLQLAPFGLSDFRALVRELTELAQQTDKGLLLAGQALESLRQKRRILPALS
                     VIDRACSEAIARANRRVYRALVEPLTDSHRAKLDELLKLKAGSSITWLTWLRQAPLKP
                     NSRHMLEHIERLKTFQLVDLPEGLGRHIHQNRLLKLAREGGQMTPKDLGKFEPQRRYA
                     TLAAVVLESTATVIDELVDLHDRILVKLFSGAKHKHQQQFQKQGKAINDKVRLYSRIG
                     QALLEAKESGSDPYAAIEAVIPWDEFTESVSEAELLARPEGFDHLHLVGENFATLRRY
                     TPALLEVLELRA"
BASE COUNT         2484 a         2863 c         2919 g         2426 t
ORIGIN      
        1 gagcctgtgt acggtaagcc agagcggcgg ttactgcctg ttcagcgaga tttccgccac
       61 ttatgtcggt tgttgctgtt ttaccgcggg taacaccgcc attcagacca tccacctgaa
      121 tgattttaat accgtcaatg gatttcattg gttctgtgga cttctctatg acggcaggta
      181 atgcatccag cagggcaaga cggaagcgga ggctggtctg ctcatccgaa agcacgttaa
      241 tggcatcgtt cagtgcgcgc tgtgcttctg cttctgccag acctttctga cgggtggcct
      301 ccgccagttc aataattgct gctgcctgga gttcggctgc ttctttttca gcctgtgcgc
      361 gaaccgtcag ttcaaccgct ttcgtttcag cttcctgagc agcagcaatc agggcaacct
      421 gtttggtacg gtcggcctcc gcagtctggc gggtggtttc cacatcctgc tgggcctgta
      481 cggcctcagc cagtgcttta cttgcccggg cttctgcctg tgactgctct tccgattttg
      541 ccgccacggc aatggctctg gcctggctgg cgatttcggt tgcctgctgt tgctcaattt
      601 cccgtatggc cacttcccgt tcggcttcga cctggcgggt acgcactatc tgctgtcgtt
      661 cgatttcggc ttcctctatc ttacgttcag ccagaattcg cgcggattct gcttcacgac
      721 gtcgttcagc ttcaatagca gcaatactgg cactctgctc tgccgtacgg gtttttaccc
      781 gttgttgttg ctccagagtc atgaaggctt cctgctgttc aatctccaga cgtcgggaga
      841 gggcatcccg gtttttttcc ctgatggcca cttcaacatc ctgctcaact tcgttgcgtt
      901 cgcggcgacg acgttctgtt tcctgggtca gaagggtcag gccttcagca tcaaaggcat
      961 tgtcaggatt aaaatgtacc cgtgcagtct ggttgaaact tgtcagcgac acactttcaa
     1021 gctccagacc gtttttggat aagtcttccg caacggtgtt ctgcacaccc tgaacaaagt
     1081 tctcgcgggc atcctgtaaa tcctgcatgc tcattctggc cgccgtggcc cgtagggcat
     1141 caacaaattt atcctcgacc agactacgca gatcttcagg tgtgagcgtg cgttgtccca
     1201 gcgtctgggc tgcagtggat attccttcgg ctgatggttt tacccgaaga aaaaaggcaa
     1261 cggcaacatc aacacgcatg cggtcgcggg ttatgagact ttcagccgct gcacgactga
     1321 cttccagctt cagggtgttc atgttgacgg gaattgtttc gtgaaaaatg ggcataacaa
     1381 ttgctccgcc actcattacc actttttggc ctcccagacc tgtgcggaca aaggcctgct
     1441 ctgccgatgc ccggcggtac aggcgggcaa atatcagccc gataacgaac agggtgagga
     1501 ccagcgctat aacggtaaac aaccatcccg gaagattaaa catagtgaga acctctgtat
     1561 atgtcgggat taaagaacat ccggccaggg attcagttcg cccagccagc gggaaccgga
     1621 aagacggcag acaataatca ctttgtctcc tcgtctgaat tctttcccgg cctccggctc
     1681 cagcaacagg taatgaacct gcccgaagcg gtctgtgaag cgaccttcac agggggaacc
     1741 cgccactgcg tgataaccag tgatggtggc catacagccg atgaatgcct cctcggtgag
     1801 cgccgttgtt tcatcacggg gtaaccaggg ggcaatcagt ctgccggtgc ggtgtacgag
     1861 aggacatgtc agcagcaggc aaagtggtgc aaggagaatg ttcggagcag gagcctgcag
     1921 cagatgaata ataagaccct gcaggagaat gccggatata ccaaaaaaac ctgccagcat
     1981 acagaggacg atcagcaggg ggatccgtcc gatgttcagc cagtccagcg cctctcctgt
     2041 cagaccctcc ggtaaatcag gtgtatccat agctgaagag agggagtgtc cgcagagcag
     2101 ggcgataagt tcagcgatac cagtaagcag aacaaaagtc agggctgcca gatagggtgc
     2161 gttatacgcg gtaagtaacg tcagtgtcat ttttcctcct gtggcaggtc agtcaaacaa
     2221 cattatggcg tgaagcttcc gcttctttaa ttgtcagaag aagcatatcg tcagtcatca
     2281 gaagacaagg ggatgtctgc aggaggggat gataacaaca gaagcctcac gccggaatgc
     2341 gctttatgac aaccggagag gacggataat aaaaaatggg acatccacat cagcaaaccg
     2401 cgatttgcga aaaaaagtca gcggagagtg gctgccgttg tggctgtccc ggtatttcag
     2461 tgtaaaagca gactgttgtc catgccccat tcctcttccg ctaaacctga atggcccaca
     2521 tgaagaatgc gtgacagcat atttctgccg ctgccgttca gactgcattc gcgcccgtgt
     2581 gcgagcataa aaccttcatc ccggaatatc tcttcaacct cagacgtgtt gatggtggcc
     2641 ggatggatta atgaaaatgc caccggtctg atcattctgc ccgtggtgga aaaggtgtcc
     2701 ccggtgctgt tcagctgcag gatatgacgt ttcccggtct gtgcgcggga aagtgtctca
     2761 ctcagtaact ggtcattcca tgcgcacgtg ccaatctgcc atcccagact cctgcacgtg
     2821 gcaagagccg agcggaagcg gtacaggtcg tataatgctg cgttattgcc actttccagc
     2881 agtgatttca gggtgtttgc taccggtatg tctgaattgg tggccagcag ttttcgcagg
     2941 gtagtgaact cttcagccat tcctctgaac tcgcgcatct gacgggcagt gtcaagccag
     3001 ttcagcaggt aacgccggcg acgccgttcg ttgtttatgt tgtccggggc ggtggagagt
     3061 gcttcgagca tattctccag taacagaatg acagggtttt cctgggatag aatggtcata
     3121 aaggcgtcct gaaaaaatgc ccgacagccg ctctccgtac accctcaggt gtcaccggtg
     3181 ccgggcattg tggaaacaga agggaaaaaa atcccggcca taaaggccgg gaaaagtcgt
     3241 caaattaaga cagcaatttt catgtcaacg ctccgtcatc agataccagg tgcaggagca
     3301 gggcgatcct cgactccggc agaggctggg catcaagccg gaggaggtac tggtaacgcg
     3361 cttttgtcag ccagagggtg gaggcagcat ccagctcctc cctggctgcc ggattgagcg
     3421 caggatccgg gtacactttc atcaaagact gaacgatatc ctccctgacg gttttgattt
     3481 caccggccat tttcacgaag tccagaaagg cactgtgacg gcgaaggtga gtgaagctgt
     3541 ggctttcggg gtctgctttc aggaaaaagt aaccgggaaa cacggcgtgt atatgtcgac
     3601 ggtaaccaca gactttatcc gtgcggcgga cctttttcag tatcaggggt gtccagggaa
     3661 ggacgtgttg ttcggaaagc caggaaaaga gatgttcgcg gtttttaccg gcagggatgt
     3721 actgtgccag ataccagttg cgttgatgta attcactgat attcaaaatc cggaacgctc
     3781 cgcgaaaatc gatccccgca ggggggcttc gatgttgttg tgaacggcga atacataaag
     3841 catgtgtcgg aaacagcggc tgacccacgt tcagcctggc ctgcagttct gacaggcaca
     3901 tgatttacgc actcttgtaa gccttgtgga cggtttcgag gcgggcgaaa accgacaact
     3961 cctgcaaaca ggattaatgg ctggaaaata tattacataa ggatgtatct gtggcaagag
     4021 cgaggataag cagttgaata gatcgttata tttgataaga cagaatatta ttctaaattt
     4081 gatgttgtgt gcgggatatt agatcgttta aaaaaattcg gcgatctctt ctactggaaa
     4141 agaagaaaaa agcagcataa aagatcgttt gtgaaattgt tcattttttg acagagttca
     4201 aaaaaacgac gctttttagg gggttaaaga gggcaactgt aactctgaga aatcgtgtcg
     4261 gcttttcaaa gcggtggaaa aggggtatat tgcggatcgt tatgcagtgg cttttgggat
     4321 cctcgcggtc cggaaagcca gaaaacggca gaatgcgcca taaggcattc aggatgtatg
     4381 gcagaaacga cggcagtttg ccggggccgg aaggctgaaa aaagtttcag aagaccataa
     4441 aggaaaaccc ccatcatctt tcctcgaaac ttggcggaat cagagaaaga taatgggggc
     4501 ctcacagaat acgggataag tatatatgaa accgtgtcag agattcaacc ctttgcagtg
     4561 cattaacgca cggcaatctg ttcccgccat aagcgacagc ttgtggcggc cctgaaccag
     4621 acctcattta ctgaaagcac ataccgtcag gtaaagaacc cgaatccggt gttcacaccc
     4681 cgtgaagatg ccggaacgct gaagttctgc gaaaaactga tggaaaaggc cgtgggcttc
     4741 acctcccgtt ttgattttgc cgcgcatgtg gcgtatgccc gttcgcgcgg cctgcgccgg
     4801 cgtatgccgc cggtgctgcg ccgccgggcg attgatgcgc tgctgcaggg gctgtgtttc
     4861 cactatgacc cgctggccaa ccgcgtgcag tgctccatca ccacgctggc cattgagtgc
     4921 ggactggcga cggagtctga ggccgggaaa ctctccatca cccgtgccac ccgtgccctg
     4981 aagttcctgt ctgagctggg gctgataacc taccagacgg aatacgaccc gacaattggc
     5041 tgtaacatcc cgaccgacat cacgttcacg ccggcactgt ttgactcact cgatatctca
     5101 gaagaggcag tggcttccgc acgccgcagc cgcgtggaat gggaaaacag gctgcgcaaa
     5161 aagcagggga tggatgcgct gggtatggat gaactgatag cgagggcctg gcgttttgtg
     5221 cgcgagcgtt tccgcagtta tcaggcagag cttaagtccc acggcatgaa acgtgcccgt
     5281 gcgcgccgcg atgcaggcag gacccgtcag gacattgtca ccctggtgaa acggcagctg
     5341 acacgtgaaa tcgcggaagg gcgtttccgg ggaagcctgg aggccgtgaa gcgcgaaatc
     5401 gaccgtcgcg tgaaggaacg catgataatg tcgcggaaca acaactacac acggctggcc
     5461 actgcctcac ccttagtttg acctcatctg aataatccgg cctgcgccgg aggcttccgc
     5521 acgtctgaag cccggcgacg cacaaaaaag cagcacagca cacaaaaaac agcctcatca
     5581 tctgccttcc ggctcgcctt gctccccctg tttttcacgc aaaacacgtc tcacagacgg
     5641 ggaattctgt ttatccacat ttaactgcaa gggacttctc cgtaaggtta caaccgttaa
     5701 tgctataaag cgccagccgc aggcgttaca gggtgcaatg tttcttttaa accccagtac
     5761 tgtatttcct ttaaactact taactacttc catttaaaaa aaagacctat tcagtgcctg
     5821 tcctgtggac agacaaatat gcacctccca cgctgacgcg cgggccccga acctcaccgt
     5881 tctgaaacca caacaaaaag acatcaggaa taaaaacacc acacaaacgc agcaccgtgc
     5941 ctacccctca taactgaaaa gcgaggccgc ccccgcccga agggcgggaa caacatcgct
     6001 tttaattatg aatgttgtaa ctaagaatcc tcatcgctgt cagtcttctg gctggaagtc
     6061 ctcagtacac gctcgtaagc ggccctgacg gcccgctaac gcggagatac gccccgactt
     6121 cgggtaaacc ctcgtcggga ccactccgac cgcgcacaga agctctgtca tggctgaaaa
     6181 cgggtatggc ttagcagggc tggggatggg taaggtgaaa tctatcaatc agtaccggct
     6241 tacgccgggc ttcggcggtt ttactcctgt gccatatgca acaacggagt gccgccttcc
     6301 atgccgctgg cgcggcatat cctggtgacg atatctgaat cgttatatac tgtgtatata
     6361 cgtagtaatg acgaggtgat aaatggcaca ggttaatatg agtttaagaa tcgacgctga
     6421 actgaaggat gcttttatgg ccgctgcaaa aagcatggac cgtaatggct ctcagttaat
     6481 tcgggatttt atgcgccaga ccgttgaacg gcagcataat acctggttcc gtgaccaggt
     6541 tgcggcagga cgtcagcaac tcgagcgcgg cgatgtgctt ccccatgaca tggtcgaatc
     6601 ttctgccgct gcatggcggg atgaaatgag caggaaggtt tccgataaat gatggagata
     6661 ttctggatca tgctggccag ccaggacagg aagcgcattc gtgagtacat tgcggagcag
     6721 aacctgatgg ccgccattga actggacgaa cggattggtt attcagccag cagcctggcc
     6781 gggcagccgt ataaaggtcg caacggtcgt gtggaaggaa cccgggaact ggttattcac
     6841 gggggaaccg cagaattcgg aaaaaatcgt acgctaagct aacggtgttc tcgtgacagc
     6901 tctttgacta ggctttctaa ggccattctg atagccctga cttcctgaaa agccatggct
     6961 aaaatttgtg cggctaaaag ggataaccga tggtaaagta agttatccct gtcgagatac
     7021 tgaaaagcgt tatcctcgtt tttcccaaaa ctgttttgcc agttcgctca gagcgctagt
     7081 taactgagcg acagatttcg cactttgcaa attattctgc ccggtctgta cattggtatc
     7141 agcagcatcg cgtatattga taatactgcg gtttatgtct tcacttactg ctccctgctg
     7201 ctcgaccgca gtcgctattt gcgcgttcat gtcggtaatt tcgttaacgc gttggccaat
     7261 tccatcaaga gctgtagctg cttcctctgc gtgagctaca ctcgtgtgcg cttgccgact
     7321 actttgctcc atgactgtaa cagcggattg cgctcgctct tgtagagcgc tgatcatgct
     7381 ttgaatatcc gttgtcgatt gctgtgtgcg agcagcaaga ctgcgaacct catcggcgac
     7441 aacagcaaaa ccacgcccct gctcaccagc acgcgcggcc tcaattgctg cgttgagtgc
     7501 caacaaattc gtttgctcgg cgatccctcg tataacgtca agaacttttg atatctcgtt
     7561 actttgacct tcaagctcat gaataacctg agtggcttgc ctaatttcac cttcaagggc
     7621 agtgattgac tggcttgtgt gggctaccag acgctggcca gatgccgtct cagtgtctgc
     7681 tcttccggcc gcatctgcag catgctgtgc attgctcgca acctcttgaa tgcttgccac
     7741 catttggttt actgccgttg ctatttgatc tgtctctgcc tgctgctcaa ctgtaagtac
     7801 attgcttgac tcaatatcct ttagtaggcc tcgggtgtgt tcgctaagcc gatttgatgc
     7861 atcacctatg cgacctacta tggcgcctgt ttcagcttgc atcattcgta aagcaaactc
     7921 tatttggcca aactcatcgg tgcgcccagt gtagagggat tgacttaatg ggttattgga
     7981 aatattcttg gctctttcaa ccagtcttcc aagaggagag agaatagcca aaacactaac
     8041 agagcttaag cttcctgaca ttaaagtggc taacaataag ctgcttattg atgtatcagt
     8101 aagcatgccg gcagccattg cgcttgatat aatactaccc catatgagca agagtatttt
     8161 cacggaaaag ctagcagcca atttcggcct cgcggccttc ccgcttctca attgagcata
     8221 taatttttcc gcagccaaaa cctgctcagg ttcaggcttg gtccttacag actggtattc
     8281 aacaatcgaa ccattcttag ctattggcgt tacataagca cttacccaat agtggtcgcc
     8341 atttttacag cgatttttta ctagccccat ccatgagcgg ccagatttta atgtactcca
     8401 catatgctca aatgcagcag gcggcatatc tgggtgtctt acgatgttgt gaggctggcc
     8461 taatagttct tcctcagtga aaccactgat tttaatgaag tcaggattaa cgtacgtgat
     8521 atggctttga ggggaggtag tcgaaagaat attggcatct tttgggagtt ctaagtttcg
     8581 acccgtcact ggtaaattct ggcgcataag aacctcaagg gttggctgtt ttattttatt
     8641 gttttcggca ttaagcccaa tttcttgagc gttacgataa agctagcatg gaaacgatag
     8701 gtgcaagcaa gttaagggtt gcatcgcgca tgtcaatcta ggctataccc taacttgatg
     8761 tcaggcaggg ccgcgccgct tcgtcagaat agagtctgct ttcccatttt ttgacacatg
     8821 cccgcgaagg ttatagattt cagcctgaca gaaatgggct ttgaggcaca acggaacaga
     8881 aagtgcactt aagccgcctt caaccaagga gacatcgtgc aggggcaccg catcggctac
     8941 gtccgggtca gcagctttga ccagaacccg gaacgccagc tggaacaaac ccaggtgagc
     9001 aaggtgttca ccgacaaggc atcgggcaag gacacccagc gcccccagct cgaagcgctg
     9061 ctgagcttcg tccgcgaagg cgatacagtg gtggtgcaca gcatggatcg gctggcccgc
     9121 aacctcgatg acctgcgtcg cttggtacag aagctgactc agcgcggcgt gcgcatcgag
     9181 ttcctgaagg agggcctggt gttcactggc gaggactcgc cgatggccaa cctgatgctg
     9241 tcggtgatgg gggccttcgc tgagttcgag cgcgcctgat ccgcgagcgg cagcgtgagg
     9301 gcatcgcctt ggccaagcag cgtggcgcgt accggggccg caagaaagcc ctgtccgatg
     9361 agcaggctgc taccctgcgg cagcgagcga cggccggcga gcccaaggcg cagcttgccc
     9421 gcgagttcaa catcagccgg gaaaccctct accagtacct ccgcacggac gactgacaca
     9481 tgccgcgtcg cttgatcctc tcggccacgg agcgggacac cctgcttgcg ctgccggaaa
     9541 gccaggatga cctgatccgc tactacacct tcaacgactc cgacctgtcg ctgatccgcc
     9601 agcgacgcgg cgacgccaac cgcctcggct tcgccgtgca gctctgcctg ctgcgctacc
     9661 ccggttacgc gctgggaacc gacagcgagc tgcccgagcc ggtcatcctg tgggtggcga
     9721 agcaagtcca ggccgagccg gcgagctggg caaagtacgg cgagcgcgac gtgacccgtc
     9781 gcgagcatgc ccaggaactg cgcacctacc tgcaactggc cccgttcggc ctgtccgact
     9841 tccgcgccct ggtgcgcgag ctaaccgagc tggcccagca gaccgacaaa ggcttgctgc
     9901 tggccggtca ggccctggag agcctacggc agaaacgacg catcctgccg gcgctgagcg
     9961 tgattgaccg ggcctgctcg gaagccattg cgcgagccaa tcggcgggtc taccgcgccc
    10021 tggtcgaacc actcacggac tcgcatcggg ccaagctgga cgagctgttg aagctcaagg
    10081 ccggcagcag catcacctgg ttgacctggc tgcgccaggc accgctgaaa cccaactctc
    10141 ggcacatgct ggaacacatc gagcggctga agacatttca gttggtggac ttgcccgaag
    10201 gcctgggccg gcacatccac cagaaccgcc tgctcaagct ggcccgcgag ggtgggcaga
    10261 tgacgcccaa agacctcggt aagttcgagc cgcagcgccg ctacgcgacc ctggccgccg
    10321 tggtgctgga gagcaccgcg accgtgatcg atgagctggt cgatctgcat gaccgcatcc
    10381 tggtcaagct gttcagcggc gcgaagcaca agcatcagca gcagttccag aagcagggca
    10441 aggcgatcaa cgacaaggtg cgcctgtact ccaggatcgg ccaggcgctg ctggaagcga
    10501 aggaaagcgg cagcgacccc tatgccgcca tcgaggcggt gattccctgg gacgagttca
    10561 ccgagagcgt cagcgaggcc gagctgctgg cccggccgga aggcttcgac cacctgcacc
    10621 tggtcggcga gaacttcgcc accctgcgcc gttacacgcc ggccttgctg gaggtgctgg
    10681 aactgcgcgc cg
//
LOCUS       DAEEKQ010000133        10686 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.134, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000133 DAEEKQ010000000
VERSION     DAEEKQ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10686
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.134"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..784)
                     /locus_tag="KLK57_003187"
     CDS             complement(<1..784)
                     /locus_tag="KLK57_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893486.1"
                     /translation="MTRIACVGITVQDRIYSLPTLPEGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTK"
     gene            957..1835
                     /gene="yihU"
                     /locus_tag="KLK57_003188"
     CDS             957..1835
                     /gene="yihU"
                     /locus_tag="KLK57_003188"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HBE0893487.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPGKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            1860..2747
                     /locus_tag="KLK57_003189"
     CDS             1860..2747
                     /locus_tag="KLK57_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HBE0893488.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESTIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            2780..3790
                     /locus_tag="KLK57_003190"
     CDS             2780..3790
                     /locus_tag="KLK57_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HBE0893489.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKMLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            3860..5293
                     /locus_tag="KLK57_003191"
     CDS             3860..5293
                     /locus_tag="KLK57_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0893490.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASGTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            5392..6741
                     /locus_tag="KLK57_003192"
     CDS             5392..6741
                     /locus_tag="KLK57_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HBE0893491.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLVLMVLRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            6731..7618
                     /locus_tag="KLK57_003193"
     CDS             6731..7618
                     /locus_tag="KLK57_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HBE0893492.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRIFPQAGPWD
                     DCFGFYNGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            7759..8358
                     /gene="yihX"
                     /locus_tag="KLK57_003194"
     CDS             7759..8358
                     /gene="yihX"
                     /locus_tag="KLK57_003194"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HBE0893493.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            8352..9224
                     /locus_tag="KLK57_003195"
     CDS             8352..9224
                     /locus_tag="KLK57_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HBE0893494.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLFSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            9221..9658
                     /gene="dtd"
                     /locus_tag="KLK57_003196"
     CDS             9221..9658
                     /gene="dtd"
                     /locus_tag="KLK57_003196"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HBE0893495.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            9703..10644
                     /gene="fabY"
                     /locus_tag="KLK57_003197"
                     /gene_synonym="yiiD"
     CDS             9703..10644
                     /gene="fabY"
                     /locus_tag="KLK57_003197"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HBE0893496.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFINQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
BASE COUNT         2462 a         2532 c         2854 g         2838 t
ORIGIN      
        1 cttttgttgg cattttttct gccaacgcta ccgcgagtgc gccgtgaaaa acatcgcctg
       61 ctccagtggt atcaaccaca ttgacagaaa atgcctcctg ttgacacaaa tgaccatctt
      121 caatccacaa cgatccttca gaacccagcg taacatacac tttacctgct gtttgtgtcg
      181 ttgcctggaa caaaccttct tcaggtgact gcaggccagt catgcgtttc agccctggtg
      241 ttgagaacac ggcgtgatcg gcaagcgcga caagtggaga aatatcctgc ggtgtcatat
      301 cggcgtctaa aagcgttgtc acacccgcaa ggcgcgctag cgagaatgct ttttcagtac
      361 cgctatgcca gcgaacatcg gccagaatca gatcataacg agagaaatcg atcgcttcta
      421 accattcggc atcagtaccg agatcaggac ttggataatt aacgataatc cgttcgccat
      481 gctgatcaac gaggatcgct gattgcgacg aacgggcgtt aggatatcgg cggcaaaatg
      541 cggtattgac gccccaaccc tcaagttctg caagtaaggt atttccacaa ctgtcatcgc
      601 cgacgcgacc gatgaaatca acctccacgc caagctttgc gattgcaaca gcggctgttg
      661 ctgccgggcc gccaccaatc tcaagataat gatttgcctg atatttcccg ccgccctccg
      721 gcagagtggg caaagaataa attctgtcct gaactgtgat acccacgcat gcaattctgg
      781 tcatatttgt cttgtcctgt ttaattttgt cgtctttggt caatttaaat gacgcaaatg
      841 atcaaattag tgatgcgtgt cgtgtttttg aacataaatt acaaatatgc tcaaaaacag
      901 acgaaaacga attttcaatt gtcattattt tgtcaatgtc agacaggaga gtattcatgg
      961 caaccattgc atttctcggg ctggggcaga tgggtagccc aatggctaac aatctattgc
     1021 aaaaagggca ttcattgcag gtatttgatg tcaatgcaca ggccgtcgat gcgctagtta
     1081 cgcagggggc cactgcggca caaaccccag cggaagcagc agcaggcgct gaatttatca
     1141 tcactatgct tcccaatggt gacattgttc gacaggttct gttgggggaa aaaggcgtct
     1201 gtgaggctgt ttcatctgac gcgttattga ttgatatgtc gactattcat cccttacaga
     1261 ccgatgcatt aatacgggag ttacaagaaa aaggcatcag tatgatggac gctcccgtgg
     1321 gcagaacgtc cgtgaatgcg attgatggca cattgctgat tttagcgggt ggcactgatg
     1381 agcagattgc acgtgcacgc ccgattctca tgtgcatggg taatgagctg gtcgaggctg
     1441 gcggcccagg aatggggatc cgcgtgaagc taattaacaa ctatatgagt attgcgctta
     1501 atgcgctgtc ctccgaggcc gctgtgttat gtgagtcact gggattaaat cttgatgtcg
     1561 caattaaggt gatgagtggt acggcagcag ggaaaggaca ttttacgacc acctggcctg
     1621 gcaaagtgct gaaaggcgat ctttcacccg ccttcatggt ggatctggcg ttgaaagatc
     1681 tgcgtattgc agttgacgtc gctcgggaga cgggggcacc gctcaatatg gggattgcag
     1741 cagaaagtta ttacgctgca gcaagtcaag acggcaaagg gcggcaggac tggagtgccc
     1801 tgctaaatca ggttcgccag caggctggtc tttaatttta tctattaaca ggagtttcta
     1861 tgtcatccca cttcacccca tttacgctga aagatatctc ccgcccaggt ggtggatttg
     1921 ccatgttagc tgtcgatcag cgtgaggcca tgcgcttgat gtttgcggca gcagggcagc
     1981 caaaacccat cgctgacagt gtactcaccg attttaaagt tgccgcgacc cgaatccttt
     2041 ctccttatgc ttctgccgtt ctggcggata aacaattttg tcttgagcaa atagtcgagc
     2101 agggcgctgt tgctaattct tgcggcctga ttgtcgctgc cgatcttttt atcccaggta
     2161 atggcatccc ggttgatagc gttgaaatag atatgtccgt cgacccgcat aaagcccgtg
     2221 aaatgggggc taaggcaatg aaattacttg tactctggcg tgaagatgaa cccgcagagg
     2281 aacgtctggc aatggttgat aaatttgttc gtcgctgccg tagtgccggg ctggtgagca
     2341 ttattgaacc tgtggtgcgt ccacctcgtc gcggttggga ctttgatcgt gaaagcacca
     2401 ttgttgccgc cgccgcagag cttggtggaa cagaggctga tctttacaaa gccgagatgc
     2461 cattaggggg caaaggcgat gaaaaaacct tacttgccgc ctgtcagcaa cttaacgatc
     2521 agatgaagat gccgtgggta attctttctt ctggcgttga tgccgatatt tttggccgtg
     2581 cagtaagtat tgccatgaaa ggtggtgcgt cgggcttcct ggctggacga gcggtttggg
     2641 cgtcggttgt tggtgcacaa gatccacaaa ctatgctgcg cgatgtctct gttcctcgtt
     2701 tacaacggtt ggcagaaatt gtcgatgaag gtatcgcgca acgctaatcc cctatggata
     2761 tcgattctgg agaataacaa tggataaaat tacccaggtt ttattttcag atattgggaa
     2821 agtcaccact caatatgttg aagtaccaca ccaggaactt aaaccgcacg aagtgcggat
     2881 tgcgcctgtg ttctatggga tatgcggttc ggatctgcat gttctgaaag gcggtcatcc
     2941 gtttgccaaa ccacctgtcg tccccggtca tgaaattgca gcgcgcgtta cggaagttgg
     3001 cagcgacgtt aaaaatgtac agccgggcga tcatgttgtg gtcgatccca tcatggcttg
     3061 catggaatgc cgagcctgca aagcaggacg ttttaatctt tgtgaaccac cgcaggttgc
     3121 tggttttcgc gcaccgggct ttgctcgctc acaacacatt gttcctgcgc gtaattgcca
     3181 tgtcgcacca gcctctttac cgctaaaaat gttggccttt gccgaaccgg cggcttgtgc
     3241 ccgtcactgc gttaaccgaa tgccgaaagc ttctctggaa agcgtactgg taattggtgc
     3301 cggaacgata ggcttatcca tcgtgcaggc actgcgcatt atgggggcag gtaagattac
     3361 cgtgattgaa cctgacgctg ccaaacgcgc gctggcgtta aagctgggcg cagcagaagt
     3421 ttgggcacca ggtgagctgg ccgcagatgt gcgatttacg ggggcgattg atgtcgttgc
     3481 agcgcaggcc acgcttaacg atgcatgtac ccgtgtatat gccggaggca ccgtcgtgtg
     3541 catgggcgta ccaagtgggc cgcgtgaaat accattaccg atgatgcaac gtttcgagcg
     3601 tgacttgctc aactctggca tgtacatccc tgaagatttc gatgctgtta tcgaatggct
     3661 ggcggatggg cggtttgata ccagtgaact ggttaccgat ttatttgcca ttgaggatgc
     3721 agcggcggca tttgaacgcg cgcagcaaaa tgactccata aaggtcatgc tgcaatttgc
     3781 gccggaatga ttttctaaat ctctgtctgg cgctgcgtca cgtcagcgcc accctacaac
     3841 aattaaaaag gacactgcga tgagtcaaat caccagtcct gcaacttatt caattagtcg
     3901 gccacaggac gtaattgata ttgttaataa gaactctgca atcaacacca gtatcggcgt
     3961 tatttttatt gctttgggcg gcatattgat tgatgcctac caggcggcga tggtggggtt
     4021 tggtaataaa tacattgccg ctcaattcgg catttcgccg ggtcttgctg caaccgtcaa
     4081 tgcatcagta ttaatcgccg cgttaattgg cggtttatta gcgaaccgag taataaaccg
     4141 ctttgggcaa aagcgggcat ttattattgg catggggctg tgcaccatcg gtgctgcagc
     4201 ggtagctatt gctcccagta tctggtgggt gctggtgtgc cgcgtcatca tgggctttgg
     4261 tttaggcatc gacttccctt tggcaaccaa tgccgtggca gagcttcgtg gttcaacgtc
     4321 gaagaaaacc ggaacgtcgg tcaacctctg gcaaatggcc tggtatgttt cgacaaccgt
     4381 tgtttatttg gtacttttgc cgctgcttct gtcgggtatc gctgaagaac aattgtggcg
     4441 ttacggaata ttcatcggag ctatttttgc agtcatcttc atgattttgc gttacttctt
     4501 tattggtgaa tccgcaatgt gggccgcacg cgtcgggcgt taccaggaag cgtgcgacat
     4561 tctgggaaaa cgttatggtg ttcaggctcg cgttgcggca tcgggtacaa cagaagcgaa
     4621 attctcggaa aaagcagaga ataaatacag tggtggatat ggcatcttat ttaatgatcg
     4681 ttaccgcaaa cgcaccattc ttggctgtgt cgtggcaacc atgcaggcgt ggcaatataa
     4741 cgccgtaggt gtttatcttc ctcttacgtt ggcgggaata ataagtggcg ggcttactgg
     4801 cgcgttgacc ggttctgccg tcgtgaatgc cctttgtggg gtgacaggcg ggatgatcgg
     4861 ctcgtttatt ctccaacgac tgggtactcg ccgacagtcg atgtatggat ttgctgttgt
     4921 gaccttagca ttgctgtcgt taggcgcact ggcaacgact aatccctggc tgtctttagg
     4981 gttattggga tcaattattt tcttccattc agcgggtcct ggtgggctgg gcatgaccat
     5041 tgccacactc tcttatcctc ccgctattcg ccctactggg gtcggatttg cccgcgctat
     5101 tatgcgcaca ggggcaattg caggactcat tttctggccg atgctgtggg gtgcgttgaa
     5161 aactgaagcg ttttactggt tggcaatcgt gccattcctg gggttcctga cctgcgtatt
     5221 gattaattgg gaaccactgg gtgcaaatgt tgatgctgag gatgcagagg ttctggctga
     5281 attgaagaaa taaattaatg cgcatcggct taaaccgatg cgcattaatt caaactgccc
     5341 acgtttagtg gttcgtatcc cagatttctc gcctaccagg agaggaaaat tatgtctgaa
     5401 aagttacccg caccgcgtga agggctttcc ggtaaagcca tgagacgtgt cgttatgggc
     5461 agtttcgcag gcgcgttaat ggaatggtat gacttcttta tttttggcac ggcagcagga
     5521 ctggtatttg caccgctgtt ttatccagac agcgatcctt ttataggtct tatagccgcc
     5581 tttgcgacct ttggtgtcgg ttttctgact cgtcctttag gtggcatcgt ctttggtcat
     5641 tttggcgata aaatcgggcg aaaaataaca cttatctgga cgctggctat tgtcggttgc
     5701 tccacatttt tgattggttt tattccgacg tatcaggaaa ttggcatttg ggcaccgctt
     5761 gtattaatgg tgctgcgttt gattcagggc tttggtcttg gtggcgagta cggcggagcg
     5821 gcattgatga ccatagaatc agcgcccgaa tcccgacgcg gatttttagg ttcactcccg
     5881 caaacggctg cgtctgtggg tattatgctg gcaaccggta tttttgctct ctgtaaccat
     5941 tttcttactt cggagcagtt tctctcctgg ggatggcgca ttccgttttg gctttctgcg
     6001 gtgatgttga tcgttgggct gtttatccgc ctgcataccg aagaaacact ggattttcaa
     6061 aagcaaaaaa cgacgaataa taaagaaaag tccgttcctc cgttgattga attattcaaa
     6121 aaacatccac gaaatatttt attggcactg ggtgcgaggc tggcggaaag tgtctcctct
     6181 aatattatta acgcctttgg tattgtctat atttccagcc aattagcatt gtcgcgagat
     6241 attcccctga cgggtatgtt gattgcctcg gcgatcggta ttttcagttg tccattggtt
     6301 ggatggttat cagatcgtat aggtcaaaaa agtctgtatt tgtcgggagc aggattttgt
     6361 gtcctgttcg cgtttccttt cttcttatta ctggatagca aaagtacgct catcatctgg
     6421 tgcagtatga tcctcggcta caacttaggt ccgacgatga tgtttgccgt gcagccaaca
     6481 cttttcaccc gcatgttcgg taccaaagta cgatataccg gcctttcttt tgcttatcaa
     6541 ttttccgcga ttcttggcgg actaagcccc cttatcgcct caagtttgct ggcgctaggg
     6601 ggaggaaaac cgtggtatgt cgctctgttt ttatttgctg tctcagttct ttcctttgtt
     6661 tgcgtctggc tgattgagcc gaccgatgaa caagaaaccg cttcttaccg ctacatcagg
     6721 gaacaatctc atgaaaactg aacgaaaaaa cgagcattac ctggcgctac aacaggcgtt
     6781 tgacgcgcca tggcctgggc ctgtaggaga gttagtgacg ctggaaaaag gcaatattca
     6841 cctgcagatt tatccgcatg atggtgccag gataacctcg ttaaaggcat ttggttctga
     6901 agtattacgc caatggcagc cgcagcgcag agcttttcag tatggctgtt tcccaatggt
     6961 gccgtgggca gggcgactgg ggaatgcgac gctgaatgct ggtgggcaat gttattcttt
     7021 acccgccaat aaaccaccac atgcgttgca tggtatggct tgttactcaa cgtgggaaat
     7081 catcgacaaa acagcagatt ccctgacttt acgtatgccc ctggcttcac cgtggccctg
     7141 gcaaggggaa gtgattcaga cttttttgct ggagaacgat gcgctggttt tgcaactgga
     7201 agtccattca tatgccgata cattcccggc ctccgcaggc tggcatccgt ggtttgcgaa
     7261 aaagctgact ccgcaaaata cagaatcgtt gcaggtgctt tttgatgcgg actggcaaga
     7321 agaagcgggt agtgatgaac tgccaactgg aaatcgtatc tttcctcaag ccggtccctg
     7381 ggatgactgt tttggttttt ataacggggt gaaggttaag ttactgtggc ctggtaaact
     7441 cgcgatgacc atgatctctt ctgcaaacag tctggtggta tttgataaac agcccgatgc
     7501 cacctgtatt aacccgctga ctcaggcacc taacgcaata aatctaacgc cggaattggt
     7561 gacaccagat aggccgctgg tcatcgaaac tcgttggcaa tttacacctg aatcttaacc
     7621 attcatctat tgcgaaaaag ccagtgaaat tactctgctt tttcgcaatc agcatcacaa
     7681 gattaccctt attgccacat agccaaaccc atcctttccc gctacagtta atttcttgtg
     7741 gcgcgaaagg aggcaaaaat gctctatatc tttgatttag gtaatgtgat tgtcgatatc
     7801 gactttaacc gtgtgctggg agcctggagc gatttaacgc gtattccgct ggcatcgctt
     7861 aagaagagtt ttcatatggg ggaggcgttt catcagcatg agcgtgggga aattagcgac
     7921 gaagcgttcg cagaggcgct gtgtcatgag atggctctac cgctaagcta cgagcagttc
     7981 tctcacggct ggcaggcggt gtttgttgcg ctgcgcccgg aagtgatcgc catcatgcat
     8041 aaactgcgtg agcaggggca tcgcgtggtg gtgctttcca ataccaaccg cctgcatacc
     8101 accttctggc cggaagaata cccggaaatt cgtgatgctg ctgaccatat ctatctgtcg
     8161 caagatctgg ggatgcgcaa acctgaagca cgaatttacc agcatgtttt gcaggcggaa
     8221 ggtttttcac ccagcgatac ggtctttttc gacgataacg ccgataatat agaaggagcc
     8281 aatcagctgg gcattaccag cattctggtg aaagataaaa ccaccatccc ggactatttc
     8341 gcgaaggtgt tatgctaaaa accattcagg acaaagccag gcaccgtacc cgtccactat
     8401 gggcctggct aaaactactc tggcaacgta ttgatgagga caacatgaca accctggcag
     8461 gtaaccttgc ctatgtgtct ttgctctcat tagtgccgct ggttgccgtt gtttttgcgc
     8521 ttttcgccgc ttttcccatg ttttccgacg tcagcattca gttacgtcac tttatttttg
     8581 ccaactttct gcctgctact ggcgatgtta ttcagcggta tatcgaacaa tttgttgcca
     8641 attccaacaa gatgaccgcc gttggggcgt gcgggctgat cgtcaccgcg ttattgttga
     8701 tgtactccat cgatagcgcg ttgaatacca tctggcgcag taaacgagcg cgaccaaaaa
     8761 tttactcgtt tgccgtgtac tggatgatct taacgctggg gccgctgctg gcaggggcca
     8821 gtctggcgat cagttcctat ttgttctccc tgcgctgggc gagcgatctc aatactgtca
     8881 tcgataacgt gctgcgtatt tttccgctgc tgttgtcgtg gatctccttc tggttgctgt
     8941 acagcattgt tcctaccatc cgcgtaccta accgcgacgc gattgtcggc gcgtttgtcg
     9001 ctgcactcct gttcgaagca ggaaagaaag gtttcgcgct ttatatcacc atgttcccgt
     9061 catatcagct catttacggc gtgctggcgg tgatccccat tctcttcgtt tgggtctact
     9121 ggacatggtg tatcgtcttg cttggcgcgg aaattactgt cactctcggg gaataccgca
     9181 aactaaaaca agcagctgaa caagaagaag acgacgaacc atgattgcat taattcaacg
     9241 cgtaacccgt gccagcgtca ccgtggaggg agaagtgacg ggcgaaattg gcgcgggact
     9301 tttggtgtta ttgggtgtcg aaaaggatga cgacgaacag aaagcaaacc gtctgtgcga
     9361 gcgtgtgctc ggctaccgca tctttagcga tgccgaaggc aagatgaatc tcaacgtgca
     9421 acaggcgggc ggcagtgtac tggtggtttc ccagtttacc ctcgccgcag ataccgaacg
     9481 ggggatgcgc ccaagtttct ccaaaggcgc atcaccggat cgcgcagagg cgttatatga
     9541 ctatttcgtc gaacgctgcc gccagcaaga gatgaacacg caaacaggac gcttcgctgc
     9601 cgatatgcag gtatcgctgg tcaatgatgg ccccgtgaca ttctggttgc aggtatgagc
     9661 ctgcttccag ggttgtcacg ggaaacaaga gagagtatcg ctatgtatca ccttcgggtt
     9721 ccacaaacag aagaagagtt agagcgttac tatcagtttc gctgggaaat gttgcgtaag
     9781 cccctgcatc aaccaaaagg ttcggaacgc gacgcgtggg atgcgatggc gcatcaccag
     9841 atggtcgtcg acgagcaggg taatctggtg gcggtaggcc gattgtatat taatgccgac
     9901 aatgaagcgt ccattcgctt tatggccgtt cacccggacg tgcaggacaa agggttagga
     9961 acgctgatgg cgatgaccct ggagtcggtg gcgcgtcagg aaggcgttaa gcgcgtgacc
    10021 tgtagcgccc gtgaagacgc ggtggagttt ttcgccaagc tgggattcat taatcagggg
    10081 gagatcacca ccccaactac cacgccgatt cgccattttt tgatgattaa acccgttgcc
    10141 actctggatg acattctgca tcgcggtgac tggtgcgcgc agctgcaaca ggcgtggtac
    10201 gaacatatcc cgttaagcga aaaaatgggc gtgcgcattc agcaatatac cgggcaaaaa
    10261 tttatcacca ccatgccaga aaccggtaat cagaatccgc accatacgtt gtttgccggg
    10321 agtttattct cgctggcgac gctcaccggt tggggactta tctggctgat gctgcgtgaa
    10381 cgccacctcg gcggaacgat tattctggcg gatgcgcata tccgctacag caagccgatt
    10441 agcggtaaac ctcatgcggt agccgacctt ggtgccttaa gcggcgatct cgaccgtctg
    10501 gcgcgcggac gaaaagcacg ggtgcagatg caagttgaaa tctttggcga cgagacgccg
    10561 ggtgcagtgt ttgaaggcac gtatatcgtt ctgcccgcga agccatttgg cccgtatgaa
    10621 gagggcggga acgaagaaga gtagcttatg gagaggggcg gtgctgcctc tctcattcat
    10681 tccggt
//
LOCUS       DAEEKQ010000134        10591 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.135, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000134 DAEEKQ010000000
VERSION     DAEEKQ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.135"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(136..1164)
                     /gene="malI"
                     /locus_tag="KLK57_003198"
     CDS             complement(136..1164)
                     /gene="malI"
                     /locus_tag="KLK57_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HBE0893497.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1339..2931
                     /gene="malX"
                     /locus_tag="KLK57_003199"
     CDS             1339..2931
                     /gene="malX"
                     /locus_tag="KLK57_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HBE0893498.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            2941..4113
                     /gene="malY"
                     /locus_tag="KLK57_003200"
     CDS             2941..4113
                     /gene="malY"
                     /locus_tag="KLK57_003200"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HBE0893499.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4217..5218
                     /gene="add"
                     /locus_tag="KLK57_003201"
     CDS             4217..5218
                     /gene="add"
                     /locus_tag="KLK57_003201"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HBE0893500.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5254..6294)
                     /locus_tag="KLK57_003202"
     CDS             complement(5254..6294)
                     /locus_tag="KLK57_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE0893501.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSCVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            6538..6663
                     /gene="blr"
                     /locus_tag="KLK57_003203"
     CDS             6538..6663
                     /gene="blr"
                     /locus_tag="KLK57_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HBE0893502.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            6928..7143
                     /gene="ydgT"
                     /locus_tag="KLK57_003204"
     CDS             6928..7143
                     /gene="ydgT"
                     /locus_tag="KLK57_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HBE0893503.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7229..7669
                     /locus_tag="KLK57_003205"
     CDS             7229..7669
                     /locus_tag="KLK57_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HBE0893504.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            7746..8327
                     /gene="rsxA"
                     /locus_tag="KLK57_003206"
     CDS             7746..8327
                     /gene="rsxA"
                     /locus_tag="KLK57_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HBE0893505.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFNGLVKL"
     gene            8327..8905
                     /gene="rsxB"
                     /locus_tag="KLK57_003207"
     CDS             8327..8905
                     /gene="rsxB"
                     /locus_tag="KLK57_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HBE0893506.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            8898..>10591
                     /gene="rsxC"
                     /locus_tag="KLK57_003208"
     CDS             8898..>10591
                     /gene="rsxC"
                     /locus_tag="KLK57_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HBE0893507.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLTQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIAR"
BASE COUNT         2430 a         2736 c         2702 g         2723 t
ORIGIN      
        1 tctcaagttg tcagaattaa acacataaac ctcctgtgaa cttcagtaaa aataaaaggc
       61 accgcctgtg tgccacattt tttatcatta accttgagga aaggacacta ccaggtatag
      121 cagataaaga gaggattatt tcgctgcaat gagccgggcg ggaataataa gattgcgtga
      181 atgcgtctct tcatgggtga ttttttgcat catgcgatcc gcaagtgtgg taccaagttc
      241 ccgcgcaggc gtgctggccc aggtaacggg tatatcatca agtgtggttg gtgtcgcatc
      301 ggtaaatgcc gccagcgaaa cttgttgctc aaagtaacga tcgactccgc tttccccgct
      361 ttgccgccct gctttcagca aaccaaacca tgcccccatc gcaatagttt cgttatagca
      421 aaccacagca ctgatggtcg ggttatgacg taaaagcgcc gtgatagctt ccgcggcttg
      481 cttctggctg gatgtgcact ccagcaccca atcgctgtga aacggcaggc caaattttag
      541 tagagttgca caatagcctc ccacccgttc tgcccgggtt aatgaggaac tttgccctcc
      601 cagccaggcg atccgctgat gcccattgcg aatgagatgc tccgtcaaca actgtgcagc
      661 ctgcatgttg tccgggcgaa ccgtatcaac atcatcaaga taactggcac gggaagcgaa
      721 aatcaccggg atagcttttt cttctgccat ccgtcgcagg tcatcgctgc ttcctgcagc
      781 cccggcaatt accacaccat cgacaccctg attcagtaac agtgaaaacc gctgtgccag
      841 ctgctcgccg tctttaccgc cgtgaagcaa aaaaaccatc cgtccctgcg cttccagagc
      901 ttccgtcaat ccggccgtca attcggcgta aaacggcgca gataaatcac ggacgatcaa
      961 accaatgacg ccgctttgcc cgccgcgcag cgccgacgcc tggcgattgc gcacaaatcc
     1021 cagctcttca atggcggcgt taacgcgttc tcctgtggcg gtagagattc gccctttgcc
     1081 actaagcacc agcgaaacgg tacttaccga cacgcccgca gccagcgcaa catcatgaat
     1141 ggttattttt ttggcggtag ccatgcagat gacctactcc ctgattatgt gacagataaa
     1201 acgttttacc ttttatttat cttatacccg ctattatcgt tgcgtaatgt gatttatgct
     1261 tcactaaaat ttgataaaac gttttatctt ctcgcgcaat ttactgaatc cagattgttc
     1321 tctacgagga gtcgttttat gacggcgaaa acagcaccga aagtcacgct gtgggagttc
     1381 ttccagcagt taggcaaaac cttcatgtta cccgtggcat tattgtcgtt ctgcggcatt
     1441 atgctcggca ttggtagttc tcttagtagc cacgatgtca tcaccctgat cccggtcctg
     1501 ggcaaccccg tgttgcaggc tatctttacc tggatgagta agattggctc gtttgctttt
     1561 agtttcctgc cagtcatgtt ctgtatcgcc atcccgctgg gtctggcacg cgaaaacaaa
     1621 ggcgtagcgg cattcgctgg cttcgtcggt tatgcggtaa tgaacctcgc ggtaaacttc
     1681 tggttgacca ataaaggcat tctgccaacc acggacgccg cggttctgaa agccaataac
     1741 atccagagca ttcttgggat ccagtcgatc gacaccggga tcctcggtgc ggtgatcgcc
     1801 ggtattatcg tctggatgct gcatgagcga ttccataata tccgcctgcc ggatgcgctt
     1861 gcattcttcg gcggtacgcg cttcgtacca atcatctcct cgctggtgat gggccttgtc
     1921 ggcctggtga ttccattagt ctggccgatt ttcgcgatgg gtattagcgg cttgggccat
     1981 atgataaaca gcgcgggtga tttcggaccg atgctgtttg gtaccggtga acgtctgctg
     2041 ttgccgtttg gtctgcatca cattctggtg gcattaattc gctttaccga cgcaggcggc
     2101 acgcaggaag tctgcggtca aaccgtcagc ggcgcattga ccatcttcca ggcgcaattg
     2161 agttgcccga ccactcacgg tttttctgaa agcgccacgc gtttcctttc gcaaggcaaa
     2221 atgcctgcgt ttctcggcgg tctgccaggt gcagcgttag cgatgtatca ctgcgcgcgc
     2281 ccggaaaatc gccataaaat taaagggctg ctgatttctg gcctgatcgc ctgtgtcgtt
     2341 ggcggcacga ccgaaccgct ggaattcctg ttcctgttcg tggcgccagt tctgtatgtc
     2401 atccacgcgc tgttaaccgg cctcggcttc actgtcatgt ctgtgctcgg cgtcaccatc
     2461 ggtaataccg acggcaatat catcgacttc gtggtgttcg gtattttgca tggtctgtca
     2521 accaagtggt acatggtgcc agtggtggcg gcaatctggt ttgtcgttta ctacgtcatc
     2581 ttccgtttcg ctatcacccg cttcaatctg aaaaccccgg ggcgcgatag cgaagtggcc
     2641 agttcaatcg aaaaagccgt tgccggtgcg ccgggtaaat caggttacaa cgttccggca
     2701 atcctcgaag ccttaggcgg tgccgacaat attgttagcc tcgataactg cattacccgt
     2761 ctgcgtttgt ctgtgaaaga tatgtcgctt gttaatgtgc aggcactgaa ggacaatcgg
     2821 gcaattggcg tagtacaact taatcaacat aacctgcagg ttgttatcgg gccacaagtt
     2881 cagtcagtaa aagatgaaat ggccggtctg atgcatactg tccaggcata aggataagat
     2941 atgttcgatt tttcaaaggt cgtggatcgt catggcacat ggtgtacaca gtgggattat
     3001 gtcgctgacc gtttcggcac tgctgacctg ttaccgttca cgatttcaga catggatttt
     3061 gccactgccc cctgcattat cgaggcgctg aatcagcgcc tgatgcacgg cgtatttggc
     3121 tacagccgct ggaaaaacga tgagtttctc gcggctattg cccactggtt ttccacccag
     3181 cattacaccg caattgatac gcagacggtg gtgtatggcc cttctgtcat ctatatggtt
     3241 tcagaactga ttcgtcagtg gtctgaaaca ggtgaaggcg tggtgatcca cacgcccgcc
     3301 tatgacgcat tttacaaggc cattgaaggt aaccagcgca cagtaatgcc cgttgcttta
     3361 gagaagcagg ctgacggctg gttttgcgat atgggcaagc tggaagccgt tttggcgaaa
     3421 ccagaatgta aaattatgct cctgtgtagc ccacagaatc ctaccgggaa agtgtggacg
     3481 tgcgatgagc tggagatcat ggctgacctg tgcgagcgtc atgatgtgcg ggttatttcc
     3541 gatgaaatcc atatggatat ggtctggggc gagcagccgc atattccctg gagtaatgtg
     3601 gcgcggggag actgggcgtt gctgacgtcg ggatcgaaaa gtttcaatat tcccgcgctg
     3661 accggtgctt acgggattat agaaaatagc agcagccgtg atgcctattt atcggcactg
     3721 aaaggacgtg atgggctttc ttccccttcg gtactggcgt taactgccca tatcgccgcc
     3781 tatcagcaag gtgcaccgtg gctggatgcc ttacgcgtct atctgaaaga taacctgacg
     3841 tatatctcag ataaaatgaa cgccgcgttt cctgaactca actggcagat cccgcaatcc
     3901 acttatctgg cctggcttga tctacgtccg ttgaatattg acgacaacgc gttgcaaaaa
     3961 gcgcttatcg agcaagaaaa agtcgcgatc atgccggggt atacctatgg tgaagaaggt
     4021 cgtggttttg tccgtctcaa tgccggctgc ccacgttcga aactggaaaa aggtgtggct
     4081 ggattaataa acgccatccg cgctgttcgc taaccccaat tgcgcaacgt aaaaaatcgt
     4141 tgcgcaatcg tggattttta ccctgctttg tttttataat ggtgcgcact tttatatcca
     4201 gaaaaagagt gcgaccatga ttgataccac cctgccatta actgatatcc atcgccacct
     4261 tgatggcaac attcgtcccc agaccattct tgaacttggc cgccagtata atatctcgct
     4321 tcctgcacaa tccctggaaa cactgattcc ccacgttcag gtcattgcca acgaacccga
     4381 tctggtgagc tttctgacca aacttgactg gggcgttaaa gttctcgcct ctcttgatgc
     4441 ctgccgccgc gtggcatttg aaaacattga agatgcagcc cgtaacggcc tgcactatgt
     4501 cgagctgcgt ttttcaccag gctacatggc aatggcacat cagctgcctg tagcgggtgt
     4561 tgtcgaagcg gtgatcgatg gcgtacgtga aggttgccgc acctttggtg tgcaggcgaa
     4621 gcttatcggc attatgagcc gaaccttcgg cgaagccgcc tgtcagcaag agctggaggc
     4681 ctttttagcc caccgtgacc agattaccgc acttgattta gctggtgatg aacttggatt
     4741 cccgggaagt ctgttccttt ctcacttcaa ccgcgcgcgt gatgcgggct ggcatattac
     4801 cgtccatgca ggcgaagctg ccgggccgga aagcatctgg caggcgattc gtgaactggg
     4861 tgcggagcgt attggacatg gcgtaaaagc cattgaagat cgggcgctga tggattttct
     4921 cgccgagcaa caaattggta ttgaatcctg tctgacctcc aatattcaga ccagcaccgt
     4981 agcagagctg gctgcacatc cgctgaaaac gttccttgag catggcattc gtgccagcat
     5041 taacactgac gatcccggcg tacagggagt ggatatcatt cacgaatata ccgttgccgc
     5101 gccagctgct gggttatccc gtgagcaaat ccgccaggca cagattaatg gtctggaaat
     5161 ggctttcctc agcgcagagg aaaaacgcgc actgcgagaa aaagtcgccg cgaagtaaca
     5221 aaaatggatg gtgcaatttg caccatccat ttttcacgca aggcacaacg tcgcgcgatg
     5281 tttggcggat tcgatgccca gctcaatcaa ctccattacc tggattgcct ggcttgccgg
     5341 aaccggattt tcaccatcgc catttaacgc atcacgaatc gccgcatagt aagccggata
     5401 attcccaggc accgtcaaca gcgtttcttc gacacgttcc tcaccttcca cgcgggtaag
     5461 tacgccatca cgcatatcgt agccccagtc ttcctgcggc agacgctcgc catttttcag
     5521 acgttcttcc tgtggatcga ggccatattt cacacaactg cctcgggatc catgcacgat
     5581 ataccgtgct gactcagcgg ctgccagcat ggtaccgtgt aaaatgactc gccgctgtgg
     5641 ataggacaag atggcgtgga aataatcggt cgactgcgct ccgggccgta actgtgccaa
     5701 atctaccgtc atgctgaccg gtaaaccaaa tagcgtaatg gcctgatcaa gaagatgcgg
     5761 tgctaaatcg taccagatac cgctgcctgg gccgccctgt tcacgccaac gatcgcgcac
     5821 ctgcggacgg aagcggtcaa aatgagactc aaagtaagca acttcaccca gcacgccttc
     5881 cgcgagcaaa ccttttagcg tcaagaaatc gctatcccaa cgacggttat ggaatacaga
     5941 cagcacacgc cccaggcttt ttgccagcgc atccagctct cgcgcttgtg acagtgtcac
     6001 ggtaaagggt ttatcaacga ccacatgttt acccgcctca agcgccgctt tggctaacgg
     6061 gaaatgggta tcgttgggtg taggaatgac aatcaggtct atgttgggat cgttaaacag
     6121 atgcttcggc tcagagacaa ccgtaaccgt tggccagtcg gcttttactt ttgtttcatc
     6181 actgctggag attaccgcca gttccagccc gggcgtgccc gcaatcaggg gcgcatggaa
     6241 ggttttgctc gcataaccat acccaatcaa cccaacacgg atgttgtcgc tcatgtcatt
     6301 gcctctcatt gaagtatgat ggctatttga cactatcctt tacccacgct caacagttta
     6361 ataacctgcc agcaataagg gatgttgttt aacttaagtc aaaaaaaata gcgaattttc
     6421 caacgacaaa agctaaatat cgcaaaaacc tcagtaaaaa tcttgctgga gctattattg
     6481 ctaagtaaca tttaccccct gaagttaatg gatcaatcaa gagagatgtg ggctgtaatg
     6541 aatcgtctta ttgaattaac aggttggatc gttcttgtcg tttcagtcat tcttcttggc
     6601 gtggcgagtc acattgacaa ctatcagcca cctgaacaga gtgcttcggt acaacacaag
     6661 taagctctgc acttgtggag cgacatgctg cccgtccggg tgcatgtttt cacttgtcgg
     6721 atattaaacc aggaatttat tatcttgttc gatgttgttg gtgattgtca gggatagtaa
     6781 agcgcgttac tcttttcaga gcagtttttc atccctgaat atttgccatc agcgcattct
     6841 tattattgta ataacacaag cgttcacttt tggtgaaatt ttatcatcac tctacccctt
     6901 tggtattaat tattttatgg accttttatg actgttcagg actacttatt aaaatttcgc
     6961 aaaatcagtt cactcgaaag tctggaaaaa ctctacgacc atcttaatta caccctgacg
     7021 gacgatcagg aactgatcaa tatgtatcgt gctgccgatc accgtcgcgc agagctggtt
     7081 tctggcgggc gtttgtttga cctcggccag gtaccgaagt ccgtctggca ctatgtccaa
     7141 taaagaaagt agcgatcggt tataaaagcc ttataataac gcctctaaaa ttagatttgc
     7201 ctgtttgcgt cgctattctg gagaacctat gaccaccaca acgccacaac gtattggagg
     7261 ctggttgctt ggcccgttgg cctggctttt agtcgctctg ttgagtacga cgctggcgtt
     7321 gttgctgtat accgctgcgt tatcttctcc gcaaacattt caaacgctcg gcggacaagc
     7381 actgacgaca caaatcttat ggggcgtttc ttttattacc gctatcgcca tgtggtatta
     7441 cacactgtgg ttgaccatcg ctttctttaa acgtcggcgt tgtgttccta aacactatat
     7501 tatctggctg cttatttccg tattgctggc agtaaaagca ttcgcttttt cacccgtgga
     7561 agatggcata gccgttcgcc agttgctgtt tactttgttg gcaaccgcat tgatcgtccc
     7621 ctatttcaaa cgctcgtcgc gggtaaaagc gacgtttgtg aatccgtaat aaccttacag
     7681 ttaacctgtt gtcgcctgct ctggattaac ggataatagg cggctttttt atttcaggcc
     7741 gaaaaatgac tgactacctg ttactgtttg tcggaactgt actggtcaat aactttgtac
     7801 tggtcaagtt tctcggtctc tgtccgttta tgggggtttc caaaaaactg gaaaccgcga
     7861 tgggcatggg gctggcaaca acgtttgtga tgacgctggc gtctatttgc gcctggctta
     7921 tcgatacgtg gattttgatc ccacttaatc tgatttacct gcgcaccctg gcatttattc
     7981 tggtgattgc tgtggtcgtg cagttcaccg agatggtggt gcgcaaaacc agcccggtgc
     8041 tttaccgtct gctggggatt tttttgccgc ttatcaccac caactgtgca gtgctcggcg
     8101 tggcgttgct gaatatcaat ctcgggcaca atttcttgca gtcggcgctg tacggttttt
     8161 ccgccgctgt cggtttctcg ctggtgatgg tgctcttcgc cgccatccgc gaacgccttg
     8221 ctgtggctga tgtcccggca ccttttcgcg gtaatgccat tgcgttaatt accgcaggtc
     8281 ttatgtctct ggcctttatg ggctttaatg gtttggtgaa gttgtaatga atgctatctg
     8341 gattgccgtt gccgccgtga gcctgctggg cctggcgttt ggcgccattc tgggttatgc
     8401 ctcccgccgt tttgcggtgg aagacgatcc ggtcgttgag aaaattgacg aaatcttacc
     8461 gcagagccag tgtggtcagt gcggttatcc cggctgtcgc ccctacgcgg aagccatcag
     8521 ctgtaacggt gaaaaaatca accgttgcgc cccaggtggc gaagctgtga tgctaaaaat
     8581 tgccgagttg cttaatgtcg agccgcagcc gctggatggc gaagcgcaag agctaacgcc
     8641 tgcgcggatg gtggcggtta ttgatgaaaa taactgtatt ggctgcacta aatgtattca
     8701 ggcttgtccg gtagacgcca tcgttggcgc tacccgtgcc atgcatacgg taatgagtga
     8761 tctctgtacg ggctgcaatt tatgtgttga tccgtgcccg acgcactgca tctcgttgca
     8821 accggtcgca gaaacacctg actcctggaa atgggatctg aacaccattc ccgtgcgtat
     8881 cattcccgtg gaacaccatg cttaagttat tctctgcatt cagaaaaaat aaaatctggg
     8941 atttcaacgg cggcatccat ccaccggaga tgaaaaccca gtccaacggt acacccctgc
     9001 gccaggtacc actgacgcag cgttttgtta ttccactgaa acagcatatt ggcgctgaag
     9061 gtgagttgtg cgttagcgtc ggcgataaag tattgcgcgg ccagccgctt acccgtggtc
     9121 gcggcaaaat gctgcctgtt cacgcgccca cctcgggtac cgttacggct attgcgcccc
     9181 actctacggc tcatccttca gctttagctg aattaagcgt gattattgat gccgatggtg
     9241 aagactgctg gatcccgcgc gacggctggg ccgattatcg cactcgcagt cgcgaagagt
     9301 taatcgagcg catacatcag tttggtgttg ccgggctggg cggtgcagga ttcccgacag
     9361 gcgttaaatt gcagggtggc ggagataaga ttgaaacgtt gattatcaac gcggctgagt
     9421 gcgagccgta cattaccgcc gatgaccgtt tgatgcagga ttgcgcggct caggtcgtag
     9481 agggtattcg cattcttgcg catattctgc agccacgcga aattcttatc ggcattgaag
     9541 ataacaaacc gcaggcgatt tccatgctgc gcgcggtgct ggcggactct aacgatattt
     9601 ctctgcgggt gattccaacc aaatatcctt ctggcggtgc taaacaatta acctacattc
     9661 tgaccgggaa gcaggttcca catggcgggc gttcatccga tatcggcgta ttaatgcaaa
     9721 acgtcggcac tgcttatgca gtgaaacgtg ccgttattga tggcgagccg attaccgagc
     9781 gtgttgtaac cctgactggc gaagcaatcg ctcgcccggg caacgtctgg gcacggctgg
     9841 ggacgccagt gcgtcattta ttgaatgatg ccggattctg cccctctgcc gatcaaatgg
     9901 tgattatggg tggcccgcta atgggcttta ccttgccatg gctggatgtc ccggtcgtaa
     9961 agattaccaa ctgtctgttg gctccctctg ccaatgaact tggcgaacca caggaagaac
    10021 aaagctgcat ccggtgtagc gcctgtgctg acgcctgccc tgcggatctt ttgccgcaac
    10081 agttgtactg gttcagcaaa ggtcagcaac acgataaagc taccacgcat aacattgctg
    10141 attgcattga atgtggggct tgcgcgtggg tttgcccgag caatattccc ctggtgcaat
    10201 atttccgtca ggaaaaagct gaaattgcgg ctattcgtca ggaagaaaag cgcgccgcag
    10261 aagccaaagc gcgtttcgaa gcgcgccagg ctcgtctgga gcgcgaaaaa gcggctcgcc
    10321 ttgaacgaca taagagcgca gccgttcaac ctgcagccaa agataaagat gcgattgctg
    10381 ccgctctggc gcgggtgaaa gagaaacagg cccaggctac acagcctatt gtgattaaag
    10441 cgggcgaacg cccggataac agtgcaatta ttgcagcacg ggaagcccgt aaagcgcaag
    10501 ccagagcgaa acaggcagaa ctgcagcaaa ctaacgacgc agcaaccgtt gctgatccac
    10561 gtaaaactgc cgttgaagca gctatcgccc g
//
LOCUS       DAEEKQ010000135        10589 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.136, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000135 DAEEKQ010000000
VERSION     DAEEKQ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10589
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.136"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            313..1611
                     /gene="kgtP"
                     /locus_tag="KLK57_003209"
     CDS             313..1611
                     /gene="kgtP"
                     /locus_tag="KLK57_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HBE0893508.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HVNVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1608..1931)
                     /locus_tag="KLK57_003210"
     CDS             complement(1608..1931)
                     /locus_tag="KLK57_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HBE0893509.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="KLK57_003211"
     CDS             complement(1977..3332)
                     /gene="pssA"
                     /locus_tag="KLK57_003211"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HBE0893510.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENSGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLKLAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3446..6106)
                     /gene="pat"
                     /locus_tag="KLK57_003212"
     CDS             complement(3446..6106)
                     /gene="pat"
                     /locus_tag="KLK57_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HBE0893511.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGSFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPTLPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="KLK57_003213"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6138..6836)
                     /gene="tapT"
                     /locus_tag="KLK57_003213"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HBE0893512.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVHRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="KLK57_003214"
     CDS             complement(6905..7324)
                     /gene="trxC"
                     /locus_tag="KLK57_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HBE0893513.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7531..8568
                     /locus_tag="KLK57_003215"
     CDS             7531..8568
                     /locus_tag="KLK57_003215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HBE0893514.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8616..9305)
                     /gene="ung"
                     /locus_tag="KLK57_003216"
     CDS             complement(8616..9305)
                     /gene="ung"
                     /locus_tag="KLK57_003216"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HBE0893515.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            9610..9993
                     /gene="grcA"
                     /locus_tag="KLK57_003217"
     CDS             9610..9993
                     /gene="grcA"
                     /locus_tag="KLK57_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HBE0893516.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10049..>10589)
                     /gene="eamB"
                     /locus_tag="KLK57_003218"
     CDS             complement(10049..>10589)
                     /gene="eamB"
                     /locus_tag="KLK57_003218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HBE0893517.1"
                     /translation="AMTPGPNNILALSSATSHGFRQSTRVLAGMSLGFLIVMLLCAGI
                     SFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQAKPISFWASFALQFVNV
                     KIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCWALAGHLFQRLFRQYGR
                     QLNIVLALLLVYCAVRIFY"
BASE COUNT         2633 a         2699 c         2722 g         2535 t
ORIGIN      
        1 aaaggccatc ctgacggatg gcctttttgc attggcgcag aaaaaaatgc ctgatgcgac
       61 actgcgcgtc ttatactccc acatatgcca gattcagcaa cggatacggt ttccccaact
      121 tgcccacttc catacgtgtc ctccttacca gaaatttatc cttaagctcc tcaataacca
      181 ttttcctgct aactaaattc atggttaagg ttgcacaatg atatgcaaca aatgtataac
      241 attcccttta ccaaaaaaaa taaacaaaag cgaccgacaa aagcatcgga ttacggcagg
      301 agacataatg gcatggctga aagtactgta acggcagaca gcaaactgac aagtagtgat
      361 actcgtcgcc gcatttgggc aattgtgggg gcctcttcag gtaatctggt cgagtggttc
      421 gatttctatg tctactcgtt ctgttcactc tacttcgccc acatcttctt cccttccggg
      481 aacacgacga ctcaactact acaaacagca ggtgtttttg ctgcgggatt cctgatgcgc
      541 ccaataggcg gttggctatt tggccgcata gccgataaac atggtcgcaa aaaatcgatg
      601 ctgctatcgg tgtgtatgat gtgtttcggc tcactggtaa tcgcctgttt gcctggttat
      661 gaaacaatcg gtacgtgggc tccggcatta ttgcttctcg cgcgtttatt tcagggatta
      721 tccgttggcg gagaatatgg caccagcgcc acctatatga gtgaagttgc cgttgaaggg
      781 cgcaaaggtt tttacgcatc atttcagtat gtgacgttga tcggcggaca actgctagcc
      841 ctactggttg tcgtggtttt acaacacacc atggaagacg ctgcactcag agagtgggga
      901 tggcgtattc ctttcgcgtt aggagctgtg ttagctgttg tggcgttgtg gttacgtcgt
      961 cagttagatg aaacttcgca acaagaaacg cgcgctttaa aagaagctgg atctctgaaa
     1021 ggattatggc gcaatcgccg tgcattcatc atggttctcg gttttaccgc tgcgggctcc
     1081 ctttgtttct ataccttcac tacttatatg cagaagtatc tggtaaatac tgcgggaatg
     1141 catgtcaacg tggcgagtgg cattatgact gccgcattgt ttgtattcat gcttattcaa
     1201 ccactcattg gcgcgctgtc ggataagatt ggtcgccgta cctcaatgtt atgtttcggt
     1261 tcgctggcag ccatttttac cgttcctatt ctctcagcat tgcaaaacgt ttcctcgcct
     1321 tatgccgctt ttggtctggt gatgtgtgcc ctgctgatag tgagttttta tacatcaatc
     1381 agtggaatac tgaaggctga gatgttcccg gcacaggttc gcgcattagg cgttggcctg
     1441 tcatatgcgg tcgctaatgc tatatttggt ggttcggcgg agtacgtagc gttgtcgctg
     1501 aaatcaatag gaatggaaac agccttcttc tggtatgtga ccttgatggc cgtggtggcg
     1561 tttctggttt ctttgatgct acatcgcaaa gggaagggga tgcgtcttta gtgacgggtc
     1621 agttgccaga cggtatagcc ggtgcttgca ccggcgacat cccaggccaa atccttccag
     1681 ctccagccgc tcccttcggg gcggctatcc caaagctctt ttgacgcccc caaactgaca
     1741 gagaacatca atccaaacat ggcactgcga tcccggctca tcccctgatg ctgtgaatat
     1801 tcatttccgg cggcggaaag catcgccgag gcgataaagt gttgagcttt atcctgcccg
     1861 ctccagctat cgttagccat atggctgcat ccagaaagta acaatagcga acagacaaaa
     1921 agaatacgca aaacgtggcc tccaaaaaac aaaccccgta caggacgggg ttgtgattac
     1981 aggatgcggc taattaatcg gtcgatgcgg atacggcgca aacggcggat gagtttacga
     2041 accttcaccg gataatcggc aatactttgc agatcgcgat agtgcttaac gatggtggta
     2101 tgctcgcgga tcagctccag ttctttctct cgctgcggcg caagcttcag ttgtggatcg
     2161 tggatcaaaa tggcgttttc cagatccaga cgccaggcgc gcgggttcag gttattaccg
     2221 gtgatcaaca tccacttatc atcaacccac atccctttca ggtgataggt gttgtcgtca
     2281 tctttccata accgaaccac tagctggtca gtattgacgt aatactgcaa acggctcagg
     2341 aaacgacgca gattgatctc atagagataa ggcaatgcgc caattatctt gaaaggttca
     2401 tcttccggaa tgtagaagtc gttcgcggtt ttatcaccaa caataatttc gacctttttc
     2461 ccttcgcgca gcaactggat aatattgcgc acaaggattg ctggcaggtt gaagtatggc
     2521 gtacagatgg ttagtttctg ctcggcacaa ggcataagat ggaaaatggt cttgttcaac
     2581 agactcgatt tccccagccc cactagcggc gttacggaaa gctgatcgtt gtcggcatcg
     2641 ccctggaaat gataagcggc atcacgcagc tcctggcgga acagacgaat atcgttcttg
     2701 atttccgggc tttttggccg attaacatca tccagacgat taacgccgcg gccattcata
     2761 atattctgtg taacccattc aaacataatg tctgacatct tacggttacg gataagatga
     2821 taacggtcgt agcgatattt atcgtgctga tgcaggtaaa catcgttcag gctggcaccg
     2881 ctataaagta cgctatcgtc gatgataaag cctttaaagt gcagaacacc aagggcttca
     2941 cgggtattga ttggaacgcc ataaaccgga acatctacgc ccgaattttc ctgcgccatg
     3001 cggcagtacc agtcagcgtt agtgttagat gccgcagcgc caatgcgtcc acgttgtgca
     3061 cgatgccagt cgaccagcac ccgcacatcc agttccggac gctgcctttt agcttcatac
     3121 aacgcgttca gaatgccttt gccaccgtca tcctgttcga gatacagggc gacaatgcaa
     3181 atgcgctgct tcgcgctggc tattttttcc agcagcgtct cccggaagtc ggcgggagcg
     3241 taaaagaaat cgacatcatc aactgattga gaaatcttgg gtagttgggc aaggtgttgt
     3301 tgatgtttat tacgcttaaa ttttgacaac atcacagtgc atttcttctc tgttcattga
     3361 agggtcctct gtgcaatgca gacgacataa gcgggcaata ataacaccag tcccgattaa
     3421 gtggtcaaca tttccagtac cttactcatg attcctcgcg ttgggcaaga tttagcgtaa
     3481 gcccaacgat cccctcttcg agctggatat caacgttaaa cccgagcttg cgggctagcg
     3541 ccaccatgcc acgattgttt ggcatcgtaa taccattcag acgttgtagt ccgtgatctc
     3601 gcgtataggt aatcaacttt tccattaagc gtcgacctaa gcctaaccct ttgagatccg
     3661 agcgaaccag cacagcaaat tcggcatcga tgttatcagg atcggaaatc gcacgcgtga
     3721 cgccgaggat ctcttccgtt tgatcaatac gtcgtaccgc tacaaacgcc atttcccgat
     3781 cgtagtcgat ctgtgtcatg ttggctaaat cttcatgggt aaattcgttg atctcgctaa
     3841 agtagcggta ataaagatct tctttggtga ctcgcgaaat gaattgctga agttgtggct
     3901 catcttctgg caaaatcggg cggaacaagc agcgttcacc gtttttcaat tctacccatt
     3961 cttccagctg atgcggataa gggcgcactg ccagccgact ctcgttatcg ccttcaaacg
     4021 gcgagatatc cagcgtgaca tccagcgcgg taaattcact gccagaagcc agcaaaggat
     4081 gaatatccag acgctgaatt tccgggcaat cgacaatcaa gttggaaacc tgcaccagaa
     4141 gctggctcaa gcctgcaaca tccaatgggc gtagcgcact gcgcgcacga atctttttac
     4201 ttttgatccc ctgaataacc agatagcggg ccaggttcat gttcagcggc ggcagtgcga
     4261 cgacggcttg atcttcagga cgccactcca caccgccttc acccagcatg atcaacggcc
     4321 cgaaaaccgg atcgtgctca accacaaccc gcaactcctg agcgccagca cggttagcca
     4381 tactttgcac caacaggccg tggacccgcg cctgtggcca ggccattttt acgcgatcga
     4441 aaatagcgtt cgccgcttgc tggacttcat tggctgtacg caggtaaagc atgacgccct
     4501 gaacttccga tttatgtgga atatccggcg aacgcaattt cagcgccacc ggataaccaa
     4561 tctgttcggc aatatgcacc gcttcggtgc tatcgctggc aatccaggta gggagcgtgt
     4621 tcatgccata cgcttgcagg atgggctgaa cttcatgggt atcgagcgac gtagcccctt
     4681 cggcaatcgc ctgttgcaac agaagatgcg cttctgcggt attggaagtc agattgctgg
     4741 gcaacgtcgg cgtttcgcgt agttgcttct gattacgccg gtactccacc atatgcataa
     4801 aagcagtgat ggttccttcc ggggtacggt aggtcggcag cccggcttcg ctgaataaac
     4861 gtcgtgcctc ttgcgaggag tgctcgccgc accagttcgt cagcaaagag acatatttgc
     4921 tgcggggatg atgctttacc gcttcaatta atacttgcgc gctttctgtt gcgggagcag
     4981 cggcgctggg cgaatgaata accatcagcg catcaaaatc ctggctgtgg agcagaatgt
     5041 ccagcgtttt gacataatgc tcgctgctgg cgtcatcgcg cagatcgaga gggttagaga
     5101 ctgccacatg ttctggcaat gcatcgcgca atttctggca ggtttcttcg cttagcgttg
     5161 ccagcttgcc attgcgtgac cataaggcat ccagcgccag cgcggcaggc gcagcaccgt
     5221 tgctgataat catcaaccgg tcgccacgca gcgggcgcat atggctaagg gtttccaccg
     5281 ccgaaaacag ttcgtgggta tcctgtaccc gcaacaaacc ggcacgctga atagccgcat
     5341 cccatgccgg gtccattcct gccgtcgtgt tgagcagtcg ctgtgccgcc gggctacgtc
     5401 cgcttttaat caccagaatc ggtttattac gcgaggcact acgggctgcc gaaacaaagc
     5461 gtcgcgcgtc gcttaactgt tcgagataga gcaggatggc gctggtttta ctgtcgcgcg
     5521 ccagatagtc gagcaattca tcaacgtcga tatccaggct gtcgccgagc gcaataaagt
     5581 aggaaaagcc catctcacgc tgttgcgccc agtcgaggat ggtgttggag acggctgccg
     5641 attgtgaaat aaacgccagc ttgccgcgtt taatcggcac aggcgaaaag ctggcattca
     5701 gaccttgcca gggagccagt aaacccagac tgtttggtcc aagcaggcgc atgttatggc
     5761 gcagggcgca ggcgcggaga tcttcgtgtt gcgatgccgg ggcggaaaga ataatgcagg
     5821 ttttacagcc tttctcgccg agctcttcca gaagagcaag attacggctg gcattggtac
     5881 ataaaaccgc aaggtcgggt gtaaagggca agctggcaat atccggccag gccaacacac
     5941 ccaacaccgc tttccaggct ggcgtcaccg ggagtaccgg tccgttaaag cttcccgcca
     6001 gcaggttacg catcatcagg taacctgcgc gattgggttt catcgacgcg ccaattaccg
     6061 ctatcgattt tggtcgcagt agtgcttcca gtcctcgctg actcataccg gtctcccgca
     6121 gaagtgaccg aatgatttta aacgctttct aactgttctg ctgtgatgct acccagatgt
     6181 tgcgtttttc ctgccagata gcgtgtttta aagcgggtaa aatgctcgcc taaccctgct
     6241 gccgccccgg tatcgccggc catatctaac agtgcgatgg ctacctcggc agtacaatat
     6301 tggcctgcag cctgggcttc acgcaggcga taggcagaaa gccgggaaag atcgacggaa
     6361 atgacgggaa gattatccag atacggactt ttacgaaaca tcttgcgagc ttccggccag
     6421 gtaccatcga gcatgataaa cagcggtggc ttaccggcag gtggtgtgaa gatcacttcc
     6481 cgttgctcat cagcatacga ggcgggaaag accaccattg gctgataatc cgggttttgt
     6541 accagatcca gcaaatcctg cgagggttcg gtacgcgacc attgaaacgc aacggtatca
     6601 ggcaaaatat cagcaatcag acgcccggta ttactgggct tcattggctc ggtgtcgaac
     6661 atcagcaaac agaagcgact ttttgcttgt gctggggtaa ttgtcgaaca gagacataat
     6721 ttctctggca aaagacagcg ttggcagcga tgaacgcgat taccgcgggc aagaaaagga
     6781 cgtgttgcgc gcgcaatacg ctcggcgcgt aactggagaa cagcgttttc ggtcataaga
     6841 gagcgtcgaa aaaacgccat tgtcgcagag gagaaaacgg ggcacaagat gcaccccggt
     6901 aagattaaag agattcgttc agccagctat cgaacggcgc tttcggtact gcgccattaa
     6961 gcatgtcgac aacctgaccg tttttgaaaa tcatgatcgt cggtatacta cgaattccaa
     7021 agcgactgct caattcacgt tcagcttcgg tattcacttt cacaaagcgc actttaccgc
     7081 tacgctcttg cgcgacatct tcaaaaattg gtgcgaaatt acggcagggg ccgcaccacg
     7141 gtgcccagaa gtcgatcacc acaggtagat catccttcag caatttgtcg agtgtttcac
     7201 cggtcgcatt aatcacctct ccgtcaaaca agtcgtgacc gcagcgtccg cattttgccg
     7261 catcttcgat ccgatcgtcg ggaatgcgat tgatggcctg acaatgggta caaacggtat
     7321 tcataactaa cctcgggatg agtaaggaga cttatcggca acgacgccgg tatgtttcta
     7381 atatgttaca tattatcggg gacattgttc cagacaacaa tctgttttat tcaataggta
     7441 tgatagtcgc aggctttggt acgaaatcat ggcgaaccgc gcggacttcg ggtaatctgc
     7501 gcgcttcgcg cagcgctggt ggagaaaagc atgaacgatg aaatgaaagg taaaagcggc
     7561 aaggtcaaag tgatgtatgt ccgcagtgat gatgattctg ataaacgtac ccacaacccg
     7621 cgtaccggga aagggggcgg gcgtccagga aaatctcgtg ctgacggtgg ccgtcgcccc
     7681 gcccgcgatg acaaacagag tcagccccgt gaccgcaagt gggaagattc gccgtggcgc
     7741 acggtttccc gcgcgccggg tgatgagacg ccggaaaagg ccgatcacgg tggcatcagt
     7801 ggtaaaagtt ttattgatcc ggaagtgttg cgtcgtcagc gtgcggaaga aacccgcgtc
     7861 tacggcgaaa atgcctgtca ggcactgttc cagagccgtc cggaagcaat tgttcgcgcc
     7921 tggtttatcc agagcgtaac gccacgtttt aaagaagcct tgcgctggat ggcggcaaac
     7981 cgcaaagcgt accatgtggt ggatgaagct gaattgacaa aagcgtcagg cacggaacat
     8041 cacggcggcg tttgcttcct gatcaaaaag cgtaacggta caaccgtgca gcagtgggta
     8101 agccaggcag gcgcgcagga ttgcgttctg gcactggaaa atgaatctaa cccgcataac
     8161 ctgggtggca tgatgcgcag ctgcgcgcac ttcggcgtga aaggtgtcgt ggtgcaggat
     8221 gcggcactgc tggagtcggg ggcggctatc cgtaccgcag aaggcggcgc agagcacgtt
     8281 cagccgatta ctggcgacaa cattgttaac gtgctggatg atttccgtca ggcaggttac
     8341 accgtagtga ccacttccag cgagcagggt aaaccgctgt tcaaaactag tctgccagcg
     8401 aaaatggtac tggtgctggg tcaggaatat gaagggttac cggatgccgc acgcgatccg
     8461 aacgatctgc gcgtgaagat tgacggtacg ggcaacgttg ccgggctgaa tatctctgtc
     8521 gcaaccggcg ttttgcttgg tgaatggtgg cgtcagaata aagcctgata aatcagccgg
     8581 gtggcaactc tgccatccgg catttccccg caaatttact cactctctgc cggtaatact
     8641 ggcatccagt caatcggcgt ctcgccacgt tgttccagcc actgatttgc cagcacaaaa
     8701 tggttgcagc caaagaatcc acgatgcgcc gaaagcggcg acggatgcgg tgctttcagt
     8761 acatgatggc gttgcttatc tataatcgcc cctttctttt gcgcatgtga tccccacaac
     8821 aaaaacacca cgccttcgcg gtgctggtta atcaggctga tgactttatc agtaaacgtc
     8881 tcccaaccga ggctggcgtg ggaatgcgcc tgacctgcgc gaaccgtcaa cacagtattg
     8941 agcagcagaa cgccctgacg cgcccagctt tcgagatagc catgattagg gcgggtgaag
     9001 cccggaatag tattttccag ctctttatac atattcaata atgacggagg aatggcaatg
     9061 ccgggacgaa cggaaaatgc cagaccatgc gcctgtcccg gtccgtgata aggatcctgg
     9121 ccgagaatca ccactttaac gtcacccaac tcggtaaagc ggaacgcgtt aaagacatct
     9181 ttttgtggtg gatagatagt gacgccggac tgccgctcgc tggcgacggt ctgaagggta
     9241 ttaagaaaat aaggttgttg cttctcttca gccagcacgt catgccaggt taattcgtta
     9301 gccatctcgc tctcctgcga atcttcaatc cgcctagctt aactgcttct ttctacagaa
     9361 caaaatcatg ctcatttttt agacttaaac gagtgcaaaa aaatgtaaat tggtaaaatt
     9421 tcacacccgc ataagttgat gtaaaacaat aaaatccatt tacgaccaca atcgatgtgt
     9481 ggtttttatt gatttaaatc aaagaatcaa gggtggttga ggagtatata tacactcaag
     9541 caacaatggt tttaccaatt ggccgcgaca ggctgaacaa atcaaataat tttgccgggg
     9601 aggcatcaca tgattacagg tatccagatt actaaagccg ctaacgacga tctgctgaac
     9661 tctttctggc tgctggacag cgaaaaaggc gaagcacgtt gcatcgttgc aaaagcaggt
     9721 tatgcagaag atgaagtggt tgcagtaagc aaactgggtg acattgaata ccgtgaagtt
     9781 ccagtagaag tgaaaccaga agttcgcgtt gaaggtggtc aacacctgaa cgttaacgtt
     9841 ctgcgtcgtg aaactctgga agatgcagtt aagcatccgg aaaaatatcc gcagctgacc
     9901 atccgtgtat ccggttatgc agttcgcttt aactctctga ctccggaaca gcagcgcgac
     9961 gttatcgctc gtacctttac tgaaagcctg taaggcttaa gtagcaagtt actaagcgga
    10021 agagggcgac ctcttccgct ttttttcgtt aatagaaaat gcgtaccgcg caatagacca
    10081 gtaacagggc aagcacgata tttaactggc gaccatactg gcgaaacaat cgctgaaaca
    10141 gatgccccgc cagcgcccag cacacattgc caaacgtccc aatcatcgcc agcaaaacgc
    10201 tgacgccaac tacccagctt agcgcctgtg tttgcggcag aacaaacgtc gacagtgccg
    10261 taacaccgta caaaatgatt ttgacgttca caaactgcaa agcaaagctg gcccaaaagc
    10321 tgattggttt tgcctgaagt ccgtcttcct ttgttgggct ggtggcgatt ttccacgcca
    10381 gccagacaat atatgccgcc cccgcccaac tcaaaaggtg taccgctgcc gggtcaatca
    10441 ctgccagtga aaatgaaatg cccgcacaca gtaacatcac aatcaaaaat cccagactca
    10501 tccctgccag cacgcgggta ctttgacgaa atccatgcga cgtagcagag ctaagggcga
    10561 gaatattgtt cggtcctggc gtcatagcg
//
LOCUS       DAEEKQ010000136        10571 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.137, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000136 DAEEKQ010000000
VERSION     DAEEKQ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10571)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10571)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10571
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.137"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..936
                     /locus_tag="KLK57_003219"
                     /pseudo
     CDS             1..936
                     /locus_tag="KLK57_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
     gene            1106..1384
                     /locus_tag="KLK57_003220"
     CDS             1106..1384
                     /locus_tag="KLK57_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HBE0893518.1"
                     /translation="MGKPDVTDSNLKNIIDDLYRPNAKVGSGSTADAVRYELATGEKV
                     GGRGHVLKAQDYTRALQDWLDKNPTASPGDRAAAENVLQDLQNALGGK"
     gene            1384..1761
                     /locus_tag="KLK57_003221"
     CDS             1384..1761
                     /locus_tag="KLK57_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893519.1"
                     /translation="MNQYIASYIEKMASFSTVLSETVSLANEYWHPETPPVVMLFSQV
                     GKTIVKNFYEMGDIEREKIFKHVEDGMVSLDDELATVVATGLIEAIVTATDSNSLLWQ
                     DLSKFLGDKSKSHALVWKNFGQE"
     gene            2230..3366
                     /locus_tag="KLK57_003222"
     CDS             2230..3366
                     /locus_tag="KLK57_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HBE0893520.1"
                     /translation="MELKKLMEHISIIPDYRQAWKVEHKLSDILLLTICAVISGAEGW
                     EDIEDFGETHLDFLKQYGDFENGIPVHDTIARVVSCISPAKFHECFINWMRDCHSSDD
                     KDVIAIDGKTLRHSYDKSRRKGAIHVISAFSTMHSLVLGQIKTDEKSNEITAIPELLN
                     MLDIKGKIITTDAMGCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAISEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCKIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAESGLS"
     gene            complement(3408..4178)
                     /gene="yafV"
                     /locus_tag="KLK57_003223"
     CDS             complement(3408..4178)
                     /gene="yafV"
                     /locus_tag="KLK57_003223"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HBE0893521.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPKMF
                     TSGFAMEAAASSLAQDDVVDWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMMALREYREKFPAWQDADEFRLR"
     gene            4359..4805
                     /gene="ivy"
                     /locus_tag="KLK57_003224"
     CDS             4359..4805
                     /gene="ivy"
                     /locus_tag="KLK57_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HBE0893522.1"
                     /translation="MFKTITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDDTYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(4848..7292)
                     /gene="fadE"
                     /locus_tag="KLK57_003225"
     CDS             complement(4848..7292)
                     /gene="fadE"
                     /locus_tag="KLK57_003225"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HBE0893523.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLDGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            7532..8110
                     /gene="lpcA"
                     /locus_tag="KLK57_003226"
     CDS             7532..8110
                     /gene="lpcA"
                     /locus_tag="KLK57_003226"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HBE0893524.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            8316..9083
                     /locus_tag="KLK57_003227"
     CDS             8316..9083
                     /locus_tag="KLK57_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HBE0893525.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(9054..9794)
                     /locus_tag="KLK57_003228"
     CDS             complement(9054..9794)
                     /locus_tag="KLK57_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HBE0893526.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(9950..10228)
                     /gene="yafQ"
                     /locus_tag="KLK57_003229"
     CDS             complement(9950..10228)
                     /gene="yafQ"
                     /locus_tag="KLK57_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HBE0893527.1"
                     /translation="MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLP
                     AVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            complement(10231..10491)
                     /gene="dinJ"
                     /locus_tag="KLK57_003230"
     CDS             complement(10231..10491)
                     /gene="dinJ"
                     /locus_tag="KLK57_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin DinJ"
                     /protein_id="HBE0893528.1"
                     /translation="MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVARE
                     KALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDLFDKLGI"
BASE COUNT         2861 a         2489 c         2615 g         2606 t
ORIGIN      
        1 attcctgttc acgataccat tgccagagtt gtatcctgta tcagtcctgc aaaatttcac
       61 gagtgcttta ttaactgaat gcgtgactgc cattcttcag atgataaaga cgtcattgca
      121 attgatggaa aaacgctccg gcactcttat gacaagagtc gccgcagggg agcgattcat
      181 gtcattagtg cgttctcaac aatgcacagt ctggtcatcg gacagatcaa gacggatgag
      241 aaatctaatg agattacagc tatcccagaa cttcttaaca tgctagatat taaaggaaaa
      301 atcatcacaa ctgatgcgat gggttgccag aaagatattg cagagaagat acaaaaacag
      361 ggaggtgatt atttattcgc ggtaaaagga aaccaggggc ggctaaataa agcctttgag
      421 gaaaaatttc cgctgaaaga attaaataat ccagagcatg acagttacgc aatgagtgaa
      481 aagagtcacg gcagagaaga aatccgtctt catattgttt gcgatgtccc tgatgaactt
      541 attgatttca cgtttgaatg gaaaggactg aagaaattat gcgtggcagt ctcctttcgg
      601 tcaataatag cagaacaaaa gaaagagcca gaaatgacgg tcagatatta tatcagttct
      661 gctgatttaa ccgcagaaaa gttcgccaca gcaatccgaa accactggca cgtggagaat
      721 aagctgcact ggcgtctgga cgtggtaatg aatgaagacg actgcaaaat aagaagagga
      781 aacgccgcag aattattttc agggatacgg cacatcgcta ttaatatttt aacgaatgat
      841 aaggtattca aggcagggtt aagacgtaag atgcgaaaag cagccatgga tagaaactat
      901 ctcgcgtcag tccttgcggg gagcgggctt tcgtaatctt tccctaggga gtcagccgaa
      961 tgttgggaag gatttgacgg atgaagagaa gaactgcttg gccggcggca gtacgggaag
     1021 cccgggagga tggggaccag aagatgagaa tagcgcaagg gatcgtatgc agcatgaacg
     1081 gtataaagat gaattgcgac agaaaatggg aaaacccgac gttacagatt caaatttgaa
     1141 aaatattata gacgatcttt atcggccaaa tgcaaaagta ggaagtggaa gcacagcaga
     1201 tgctgttcgt tatgaattgg ccacaggtga aaaagttggt ggtagaggac atgtattgaa
     1261 agcacaggat tatacaaggg ctttacagga ttggttggat aagaatccaa cagcatctcc
     1321 aggggatcga gcagcagcag aaaacgttct tcaggattta cagaatgctt tgggaggcaa
     1381 gtaatgaacc aatatattgc tagttatata gaaaaaatgg catcattttc cactgttttg
     1441 tctgagacag tctcattagc taatgaatac tggcatccag aaacgccacc ggtggttatg
     1501 ttgttctctc aggttggaaa aacgattgta aaaaattttt atgaaatggg ggatattgaa
     1561 agagaaaaaa tatttaaaca tgtcgaagat ggtatggtct cgcttgatga tgaattggcc
     1621 acagttgtag ctacagggct tatagaggca atagtcactg cgaccgatag caattcttta
     1681 ctttggcaag atttaagtaa atttctgggt gacaaatcaa agtctcatgc attagtgtgg
     1741 aaaaattttg gtcaggagtg atattattca agaagtatta agctcgctat tgagatctta
     1801 atgcttcttg agattaaatt aaagttagga cgaagtaatt gcaggtggaa ataaatccat
     1861 agtgacgtgt ggcaatggct attagatgtt atggtgtgat gggctttggt gatttaagag
     1921 ctaagttaga ttgccttatg aaaaagtatg gttatatatt taatgatgtg tatcatatat
     1981 ttaaataaaa aacggttctc tcttgtcaag atagtaatag gatagactat tgtgttgata
     2041 aatataatta agagtctact ttgttgattt tagaagactt tggagcaggg aaagatcatc
     2101 ggaagtacac tttttgtact aaataattcg cgctaaatgt tcaataattg agatattcct
     2161 tattacctaa aactattttt catagcttat atatgatcaa atactccttg cataaataag
     2221 gagaacaaaa tggaacttaa aaaactgatg gaacatattt ctattatccc cgattacaga
     2281 caagcctgga aagtagaaca taaattgtca gatattctac tgttgactat ttgtgccgtc
     2341 atttctggtg cagagggttg ggaagatata gaggattttg gtgaaacaca tctcgatttt
     2401 ttgaagcaat atggtgattt tgaaaatggt attcctgttc acgatactat tgccagagtt
     2461 gtatcctgta tcagtcctgc aaaatttcac gagtgcttta ttaactggat gcgtgactgc
     2521 cattcttcag atgataaaga cgtcattgca attgatggaa aaacgctccg gcactcttat
     2581 gacaagagtc gccgcaaggg agcgattcat gtcattagtg cgttctcaac aatgcacagt
     2641 ctggttctcg ggcagattaa gacggatgag aaatccaatg agattacagc cattcctgaa
     2701 cttcttaaca tgctggatat taaaggaaaa atcatcacaa ctgatgcgat gggatgccag
     2761 aaagatattg cagagaagat acaaaaacag ggcggtgatt atttattcgc ggtaaaagga
     2821 aaccaggggc ggcttaataa agcctttgag gaaaaatttc cgctgaaaga attaaataat
     2881 ccagagcatg acagttacgc aatcagtgaa aagagtcacg gcagagaaga aatccgtctt
     2941 catattgttt gcgatgtccc tgatgaactt attgatttca cgtttgaatg gaaagggctg
     3001 aagaaattat gcgtggcagt ctcctttcgg tccataatag cagaacaaaa gaaagagcca
     3061 gaaatgacgg tcagatatta tatcagttct gctgatttaa ccgcagagaa gttcgccaca
     3121 gcaatccgaa accactggca cgtggagaat aagctgcact ggcgtctgga cgtggtaatg
     3181 aatgaagacg actgcaaaat aagaagagga aatgcagcag aattattttc agggatacgg
     3241 cacatcgcta ttaatatttt gacgaatgat aaggtattca aggcagggtt aagacgtaag
     3301 atgcgaaaag cagctatgga cagaaactat ctggcgtcag tccttgcgga gagcgggctt
     3361 tcgtagtctt accccgactc tcccccagcc ttaaacacaa cccccactca ccgcaaccta
     3421 aactcatccg catcctgcca tgccggaaac ttttctctat attcccgcaa tgccatcatc
     3481 gacagctccg catcaatgcg cgttgcctga tgcgcgtcgg cagtagcgat aatctcgcct
     3541 tgtggattaa tcacccggct gtcaccgcga taatggcagc cgttgccatc gctgccgacg
     3601 cgattgcatc ccgccacata cgcctgattc tcaatcgcgc gggccgtcag caatgcctgc
     3661 cagtgcagag agcgcggagc aggccagttg gcaacgtaga tggcgaggtc ataatcgttg
     3721 agattgcgcg accacaccgg aaaacgtaag tcgtagcaca ccaacggcaa aatacgccag
     3781 ccgcgccatt ccacaatcac tcgcgcattg cccgctttat aatgtagatg ctcatctgcc
     3841 atgcggaaca gatgacgctt atcataaaaa tgtaccgtgc cgcccggctc aaccagcaaa
     3901 aagcggttaa ccgaaccaga ctccgtttgt aatgcaacac tgcctgcaat cagcgcattg
     3961 cactgctgcg ccttagctgt catccagtcc actacgtcat cttgtgctag cgacgaagct
     4021 gccgcttcca tggcaaagcc gctggtaaac atcttcggta gaacgatcac atcgcgcccg
     4081 gtaatacctt ccagttgacg atcaaaatga cgcaggttgg cagggccatc catccacacc
     4141 agtggttgct gcaaaagcgt aatcttcaaa ccaggcacgg tgtacaactc ctttatgcga
     4201 agggttttat aactttaaca ccttatcagg cagttgcctt agcgcagaat aaattgataa
     4261 caaatgctga tattggaaat atctgatttg caaattatcg tgttatcgcc aggctttaga
     4321 aggttaataa catgggcagg ataagctcgg gaggaatgat gtttaagaca ataacgacag
     4381 tcgccgctct ggtcatcgcc accagtgcaa tggcgcagga tgatttaacc attagcagcc
     4441 ttgcaaaggg cgaaaccacc aaagctgcat ttaatcagat ggtacaaggg cataagctgc
     4501 ctgcctgggt gatgaaaggc ggtacttata ctcccgcaca aaccgtaacg ttgggagatg
     4561 atacgtatca ggtgatgagc gcgtgcaaac cgcatgactg tggctcgcaa cgtatcgctg
     4621 tgatgtggtc cgagaaatct aatcagatga cggggctgtt ctcgactatt gatgagaaaa
     4681 cgtcgcaaga gaaactcacc tggctgaatg tgaacgatgc gctttcgatt gatggtaaaa
     4741 cggtgctgtt cgcggcgttg accggcagcc tggaaaacca tccggatggc tttaatttta
     4801 aataattagc ggataaagaa acggagcctt tcggctccgt tattcaatta cgcggcttca
     4861 actttccgca ctttctccgg caactttacc ggcttcgtcg ccagctcttc cggatcaaag
     4921 tcatcaacgt taatactgcg cagacggctt tcttcagctt tcaccagaat agcggcttca
     4981 tctttatcaa tcagcccctt cgccagcgcg ttgtgcgcca gttcatccag acgggtaaac
     5041 ggcaggtttt tacccagctc tttacagatc cgctgatgaa ttgggtcggc ggcaatcaca
     5101 tccaccagcg cctcttccag caagccaacc ggattatgct cgctcggcgt caggtactga
     5161 ccgcgaccaa tgcgggaacg ggtggcgttc ggcacttgta aaatcttcgc cactttatga
     5221 tccagcttgt cagaaggtgc cagataatga cgtccggtcg ggaagatcac cacattcagc
     5281 agcccggcaa ccacgcggtt cgggaagttt tgcagtaaat catccatcgc ctgttcagcc
     5341 tgatacagcg catcttgtac gccccagtgc accagcggca ggtcggcttc attacggcct
     5401 tcgtcgtcat aacgcttcag cacggcagag gcgaggtaga gctggcttaa aatatccccc
     5461 agacgggccg agatgcgctc gcgacgtttc aggctgccgt ccagcactgc catcgagaca
     5521 tcagaaagca gggcgaggtt ggcgctcagg cggttcaggt gctgatagta gcgtttagtg
     5581 gcatcgccgg ttggcgtgct gctggttaaa ccgcgcgtca ggcccagcca gaagctgcga
     5641 actttgttgc taccgacgtg accgatatgt ttgaacaaca gtttatcgaa cgcgttgacg
     5701 tcattgttct tcgccgcttc catctcttcc agcacgtacg gatggcaacg aatcgctcct
     5761 tgtccgaaga tcatcatgct gcgggtcaga atgttagccc cttcaacggt gatggcaatc
     5821 ggtgcgccct ggtaagcacg cgccaggaag ttgctttgcc cgagcataat gcctttgcca
     5881 ccagtgatat ccatcgcatc aataatcgac tgctgcccgc ggtgggtaca gtgatactta
     5941 acgatagccg acagcacggc aggtttttcg ccgagcataa tgccgtaggt aatcagcgat
     6001 gccgcagcat ccatcacgta ggcattaccg gcaatacgcg ccagcggctc ttcaatccct
     6061 tccatcttac cgatagagat tttgaactga cggcgaatgt gagcatacgc gcccgttgcc
     6121 agcgctaccg atttcacgcc gccggttgag ttggaaggca gggtgatgcc gcggcctacc
     6181 gagaggcact ccaccagcat ccgccagcct tgcccggcca ttttcggccc accgatgatg
     6241 taatcgatcg gtacgaagac atctttaccg cgcgtcggtc cgttctggaa cggtacgttc
     6301 agcgggaagt ggcgacgacc aatttccacg cccggcgtgg tggtcgggat cagcgcacag
     6361 gtaataccta aatcttctgc gccacccagc agcttttctg ggtcggagag tttaaacgcc
     6421 agcccaagca cagtcgcaat cggtgccagc gtaatatagc gtttgttcca ggtcagacgc
     6481 atccccagca cctgctggcc ctgccattcg cccatgcaga caatcccggt gtccggaatc
     6541 gcgcccgcat cggaacccgc ttccgggctt gtcagtgcaa agcaagggat ctcctgacca
     6601 cgcgccagac gcggcagata gtgatctttc tgctcgtcag tgccgtaatg ttgcagcagc
     6661 tcgcccgggc ctaatgagtt cggcacgccg acggtaatcg ccaggatccc gctcacgccg
     6721 gagagttttt gcagcacgcg agactgggca taagctgaga actccagtcc gccgtactct
     6781 tttttgataa tcatcgcgaa gaagcgatgc tctttaaggt acgcccacaa ctccggcggc
     6841 agatccgcca gctcatgagt aatctggaaa tcattcgcca tccggcaggc ttcttctacc
     6901 gggccgtcga gaaacgcttg ctcttcggcg gtcaggcgcg gctgcggata gttatgcagc
     6961 tttttccagt ccggcttgcc ctggaacaag tcgccctccc accaggtggt gcccgcatca
     7021 atcgcttctt tctcagtgcg cgacatcggc ggcatcacct tacggaaacc gcgaaatacc
     7081 ggcgcggaaa tcatcgactt acgcataggc gcaaagttaa atggcacgag gataatggcc
     7141 agaggcacca gtacccacgc tgaccacaga ccagcaacgc cgagggcggc tgtccaggcg
     7201 agcaaaatca gactgctgat aaataagctc acgcggtgat agaacaacgc gccgagcagg
     7261 acaaccgtag cgagaatact caaaatcatc ataacgaaaa gccccttact tgtaggaggt
     7321 ctgaccactt gtgatgatat ggttgtagtg gatgtaaaaa catttagcaa tgtgtttaca
     7381 atataattac aacaaagctc acattgttgc tgtttttatc cgcacttcag gtcaaaaagt
     7441 cctggtcata gcacctgccc gtacttctcg cttttggcgg tatccggtac actgcatttt
     7501 gtctattaca tttatgctga aggatatcct catgtaccag gatcttattc gtaacgaact
     7561 gaacgaagcg gcggaaacgc tggctaactt tttaaaagat gacgccaata ttcacgccat
     7621 tcagcgcgcg gcggtcctgt tagcagacag ctttaaagcc ggtggcaaag tgctttcctg
     7681 cggcaacggc ggttcccatt gcgacgctat gcactttgcc gaagagttga ccggtcgcta
     7741 ccgtgaaaac cgtccgggct acccggcgat tgctatttct gacgttagtc atatttcctg
     7801 cgtcggtaat gatttcggtt tcaatgatat tttctcccgc tacgttgaag cggtaggtcg
     7861 cgaaggcgat gtactgctgg ggatctccac ctccggtaac tctgcaaacg tgatcaaagc
     7921 gatcgcagcg gcgcgtgaga aggggatgaa agtgatcacc ctgaccggta aagacggcgg
     7981 taaaatggct ggcacggcgg atatcgaaat tcgcgttccg cactttggtt atgccgaccg
     8041 cattcaggag attcacatta aagtgatcca tatcctgatc cagttaattg aaaaagagat
     8101 ggttaagtaa gtctggcgta ggccggataa ggcgtttacg ccgcatccgg cacttgtgcg
     8161 ctgatgcctg atgcgacgct gatgcgtctt atcatgccta caaatctgta cgcgaaccgt
     8221 aggccgaata agacgtttac gccgcatccg gcctgaaaat tcttaaatca atcttcgccg
     8281 ggggccatgc gctcccgctg ttgtggaggt tacccatgtg cgaattgctc gggatgagcg
     8341 ccaacgtccc taccgatatc tgctttagtt tcaccggtct ggtgcagcgt ggtggtggaa
     8401 ccgggccaca taaagatggc tggggcatta ccttttacga aggtaaaggc tgtcgcacat
     8461 ttaaagatcc acaacccagc tttaattccc ccatcgccaa acttgtccag gactacccga
     8521 taaaatcctg ttcggtggtg gctcatattc gccaggccaa tcggggcgag gtggcgctgg
     8581 aaaatactca cccgtttacc cgtgagttat gggggcgtaa ctggacttat gcccataacg
     8641 gacaactgac gggctacaaa tcactggaaa ccggcaactt ccgcccggtc ggtgaaaccg
     8701 atagcgaaaa agccttctgc tggctcctgc ataaattaac gcagcgttac ccgcgcacac
     8761 cgggcaacat ggcggcagtg tttaaatata tcgcctcact ggcggatgaa ctgcggcaga
     8821 agggcgtttt caacatgctg ctttctgacg ggcgctatgt gatggcgtat tgctcgacta
     8881 atttacactg gatcacccgc cgcgcgccgt ttggcgtggc aacgctgctg gatcaggatg
     8941 tggaaatcga cttcagctcg cagaccacac cgaatgatgt ggtcacggtg attgcaacac
     9001 agccgctgac gggcaatgaa acctggcaaa agattatgcc aggcgaatgg cgcttatttt
     9061 gcctcgggga gcgtgtagtt tgatgccagt tgtggctgca caacttcgtg gcttaacggc
     9121 ttgctgacca cgtaacgacc attgaccaca gaaacggtcg gtggcttacg ggtttgctca
     9181 aagtagtcgt aacccggctt cagttgctcc cagaagtcct taaagttgga atatttatgg
     9241 cgcttcatat tggcgtcggt catgcggaac gggtaaatac tcacttgcac gctcggctga
     9301 ccaaacacca gcgcaccagt aacgaactgg aagatctcat caataccctg attggtcatt
     9361 gcgtagcagc cgatggaaac acaatcgccg tggatcatca ggtatttccc ttcataacca
     9421 tgcgcacggt cataggcatt ggggaaacca atattaatcg ctttgtagta acggctgtct
     9481 ggttttaact gattacgctg gacgctataa aatccttccg ggcttttgaa atcgccctga
     9541 cgctgttttg gccctaagcc gccggaatat ttacagattt tataactgtc gagcagctga
     9601 tattgctcgc ccattttgac gtagagatcg agcgtacgtt cttccttgaa gatctggatg
     9661 tagacagggg atcccatcaa ctgctgctta tactctttgc tcactggcgt ggtcgagcta
     9721 ctgctgccca gcaaaccggc aaacgaaacg cacgggatca acaacatcgc aagaattaat
     9781 gcgattttac gcatactgct tattccttga taaaacggtt acacacgcca ggacggcaaa
     9841 atagatccca aatcggaata gtctggattt ggaaggctca cattatcacc aaaagagttt
     9901 tacgcaagcc tgtcggcgca gggtttacaa atttcatcgg cagcgtgctt tacccaaaga
     9961 gcgccgcgtg agttccagtt ctctcaaatc gtaaaagttt atcggtaagt ttgtaaatca
    10021 ggatccagtc cggttcgaca tgagcatcgc gataaccttt ccatgaacct tgcagcgggt
    10081 ggtctttata aacagctgga agcggtaaag cattattgat aagaagcgtc ataagatatt
    10141 tcaatttatt catatcctta tgacgctttt gtgcaagttt tacatccttt gaaaattgtc
    10201 ccgagtattc aatatccctt tgaatcatat ttaaattcct aatttatcaa ataaatcatc
    10261 agcgtctttg gccttatgaa catcaacgcc agcttcgctg tttttgattg attgaatggt
    10321 taattgatta ggctcgcgta aatcaaacgg caatgccttt tcacgcgcga cctttgtgag
    10381 ggttatgcga accaggtcag agatggtcag ccccatcccg gccagtacgt ccgccgcctg
    10441 attcttcaga tcttcatcga ttcgggcgcg aacaaacgcg ttagcagcca ttgggggatc
    10501 tcctgtgctg tatatttatt cagcttgaat tgtagctcaa atgagcaaca gcgacaatcc
    10561 atgagtaatt t
//
LOCUS       DAEEKQ010000137        10440 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.138, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000137 DAEEKQ010000000
VERSION     DAEEKQ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10440
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.138"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            142..2010
                     /gene="kup"
                     /locus_tag="KLK57_003231"
     CDS             142..2010
                     /gene="kup"
                     /locus_tag="KLK57_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HBE0893529.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAMHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            2177..2596
                     /gene="rbsD"
                     /locus_tag="KLK57_003232"
     CDS             2177..2596
                     /gene="rbsD"
                     /locus_tag="KLK57_003232"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HBE0893530.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2604..4109
                     /gene="rbsA"
                     /locus_tag="KLK57_003233"
     CDS             2604..4109
                     /gene="rbsA"
                     /locus_tag="KLK57_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HBE0893531.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVIALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLCFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGAQPRTSGYVTLDGHEVVTRSPQDGLANGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            4114..5079
                     /gene="rbsC"
                     /locus_tag="KLK57_003234"
     CDS             4114..5079
                     /gene="rbsC"
                     /locus_tag="KLK57_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HBE0893532.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            5104..5990
                     /gene="rbsB"
                     /locus_tag="KLK57_003235"
                     /pseudo
     CDS             5104..5990
                     /gene="rbsB"
                     /locus_tag="KLK57_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            6116..7045
                     /gene="rbsK"
                     /locus_tag="KLK57_003236"
     CDS             6116..7045
                     /gene="rbsK"
                     /locus_tag="KLK57_003236"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBE0893533.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            7049..8041
                     /gene="rbsR"
                     /locus_tag="KLK57_003237"
     CDS             7049..8041
                     /gene="rbsR"
                     /locus_tag="KLK57_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HBE0893534.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="KLK57_003238"
     CDS             complement(8007..9434)
                     /gene="mdtD"
                     /locus_tag="KLK57_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HBE0893535.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVSFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(9457..10149)
                     /locus_tag="KLK57_003239"
     CDS             complement(9457..10149)
                     /locus_tag="KLK57_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HBE0893536.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLNLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         2553 a         2517 c         2760 g         2610 t
ORIGIN      
        1 aagggggggt atttttcttt gagccaggtt aatgtggccg catttaggag tacgattttg
       61 ccgttaatcg tgcatactgt gcgctttttt gtgggccaag ggactaagca cacatttcat
      121 atttcaacga aagactagtc tatgagcact gataataagc aatcattgcc cgcgattacc
      181 ctcgcggcga ttggagttgt ctacggcgat attggtacca gcccgttata tacacttcgt
      241 gaatgtttgt ccggccagtt tggttttggc gttgaacgcg atgccgtgtt tggcttttta
      301 tcgctgatct tctggctgct aatctttgtg gtttccatta aatatctcac cttcgtgatg
      361 cgggcagata acgccggtga gggggggatc ctgacgttga tgtcgcttgc cgggcgtaat
      421 acgtcggcgc gaaccacatc aatgctggtg attatggggc taatcggcgg cagctttttc
      481 tatggtgaag tcgtcataac acccgctatt tcggtgatgt cagccattga aggtctggaa
      541 atcgtcgccc cgcagctgga tacctggata gttcccctct caattatcgt tctcacttta
      601 ttatttatga ttcaaaagca tggcacggct atggtcggta agctgtttgc gccgatcatg
      661 ctgacctggt ttttgattct ggcaggactg gggttacgta gcattattgc taacccggaa
      721 gtgctgcatg cgctgaatcc gatgtgggcg atgcatttct tccttgaata caaaacggtt
      781 tcttttattg cattaggggc agtggtgttg tcgattacgg gggtcgaggc gctgtatgct
      841 gatatggggc actttggtaa gttccctatt cgcctggcgt ggttcaccgt cgtattacct
      901 tccttgaccc ttaattactt cggtcaggga gcgctgttgt taaagaaccc ggaagcgatt
      961 aagaacccgt tcttcctgtt ggcaccggac tgggcgctga tcccgctgct gatcatcgcc
     1021 gcactggcga cggtaattgc ctcgcaggcg gttatctctg gcgtcttctc attgacgcgt
     1081 caggcggtac gtctgggata tttgtcgccg atgcgcatta ttcacacctc cgaaatggag
     1141 tcagggcaaa tctatattcc gtttgtgaac tggatgctct atgtcgcggt cgtgattgtg
     1201 attgtcagct ttgagcactc cagcaacctg gcggcggcgt acgggattgc ggtgaccgga
     1261 accatggtgc tgacgtctat tctctcgact accgtggcac gtcagaactg gcactggaat
     1321 aagtattttg ttgcgctgat cctgattgct ttcctttgtg tcgatattcc attgttcacc
     1381 gctaacctcg ataaactgct ctccggcggc tggttgccat tgagcctcgg tactgtgatg
     1441 tttatcgtga tgaccacctg gaagagcgag cgtttccgct tgctgcggcg gatgcatgaa
     1501 catggtaact ctctggaagc gatgattgct tcgctggaga aatcaccgcc tgttcgcgtg
     1561 cccgggaccg cggtgtatat gtcgcgtgca atcaacgtca ttccctttgc gctgatgcat
     1621 aaccttaaac ataacaaggt attgcatgag agggtgattc tgttaactct gcgcaccgaa
     1681 gacgcgccat atgtccataa cgtccgtcgg gtacagattg aacaactgtc gcccactttc
     1741 tggcgcgtgg tggcaagtta tggttggcga gaaacgccaa acgtggaaga agttttccac
     1801 cgctgcggtc tggaaggatt aagttgccgg atgatggaaa cctccttctt tatgtcgcat
     1861 gagtcgttga tcctcggcaa acgcccgtgg tatttgcgtc tgcgcggcaa gctgtacttg
     1921 ctgctgcaac gtaatgcgct gcgtgcacca gatcaatttg aaatcccgcc aaacagggtt
     1981 atcgaactgg gtactcaggt cgaaatctaa cgctagacgc ctcctttctc cataaggggg
     2041 cgtttttgtt ttcatggtta atcaccatgt aaaacgtttc gaggttgatc acatttccgt
     2101 aacgtcacga tggttttccc aactcagtca ggattaaact gtggatcagc gaaacgtttc
     2161 gctgatggag aaaaaaatga aaaaaggcac cgttcttaat tctgatattt catcggtgat
     2221 ctcccgtctg ggacataccg atacgctggt ggtgtgtgat gctggtttac ccatccccaa
     2281 aagtacaacg cgtatcgata tggcattaac ccagggtgta ccttctttta tgcaggtgct
     2341 gggcgtcgtc acaaatgaaa tgcaggtcga ggctgccatt atcgcggaag agatcaaaca
     2401 acataatccg caactccacg aaacgttgct cactcacctt gagcagctgc aaaaacacca
     2461 gggaaatacc attgaaattc gttacaccac gcatgaacaa ttcaaacaac aaaccgcaga
     2521 aagtcaggcg gtaattcgca gcggagaatg ttctccgtat gcgaatatca ttctctgtgc
     2581 tggcgtgacg ttctgaggcc gtcatggaag cattacttca gcttaaaggc atcgataaag
     2641 ccttcccggg cgtaaaagcc ctctcgggcg ctgcgttaaa tgtctatccg ggccgcgtga
     2701 tagcgctggt gggcgaaaac ggcgcgggta aatccaccat gatgaaagtg cttactggta
     2761 tctatactcg cgatgccggc acgcttttat ggctggggaa agaaacgaca tttaccgggc
     2821 cgaaatcttc ccaggaagcc gggattggga ttatccatca ggaactgaac ctgatcccgc
     2881 agttgaccat tgccgaaaac attttcctcg gtcgtgagtt tgttaatcgc tttggcaaaa
     2941 ttgactggaa aaccatgtat gccgaagcgg ataaattgct ggctaaactt aacctgtgct
     3001 ttaaaagcga caagctggtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg
     3061 ccaaagtgct gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga
     3121 ccgataccga aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccgcg
     3181 gtattgtcta tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg
     3241 tttttcgtga cgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc
     3301 tgattgagat gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc
     3361 cgggagatat ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt
     3421 ttactttacg caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg
     3481 aactgatgaa agtgctctac ggcgcacagc cgcgcaccag cggttacgtc accctggatg
     3541 ggcatgaagt cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct
     3601 ccgaagaccg taaacgtgac ggtttagtgt tgggcatgtc agtaaaagag aacatgtcgc
     3661 tgacagcgct gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc
     3721 aggcagtgag tgatttcatt cgtctgttta atgtgaaaac accgtcgatg gaacaggcaa
     3781 ttggtctgct ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac
     3841 gccccaaagt gttgatcctt gatgaaccta cccgtggcgt agatgtcggc gcgaaaaaag
     3901 agatctatca actgattaac cagttcaaag ccgatggctt gagcatcatt ctggtgtcat
     3961 cggagatgcc agaagtatta ggcatgagcg atcgcatcat cgtcatgcat gaagggcatc
     4021 tcagcgggga atttactcgt gagcaggcca cccaggaagt gttaatggct gccgctgtgg
     4081 gcaagcttaa tcgcgtgaat caggagtaaa aaaatgacaa cccagactgt ctctggtcgc
     4141 cgttatttca cgaaagcgtg gctgatggag cagaaatcgc ttatcgctct gctggtgctg
     4201 atcgcgattg tctcgacgtt aagcccgaac tttttcacca tcaataactt attcaatatt
     4261 ctccagcaaa cctcggtgaa cgccattatg gcggtcggga tgacgctggt gatcctgacg
     4321 tcgggcatcg acttgtctgt aggttctctg ttggcgctaa ccggcgcagt tgctgcatct
     4381 atcgtcggca ttgaagtcaa tgcgctggtg gctgtcgctg ctgctctcgc gttaggtgcc
     4441 gcaattggtg cggtaaccgg ggtgattgta gcgaaaggtc gcgttcaggc gtttatcgct
     4501 acgctggtta tgatgctttt actgcgcggc gtgaccatgg tttataccaa cggtagccca
     4561 gtgaataccg gctttactga gaacgccgat ctgtttggct ggtttggtat tggtcgtccg
     4621 ctgggcgtac cgacgccagt ctggatcatg gggattgtct tcctcgcggc ctggtacatg
     4681 ctgcatcaca cgcgtctggg gcgttacatc tacgcgctgg gcggcaacga agcggcaacg
     4741 cgtctttctg gtatcaacgt caataaaatc aaaatcatcg tctattctct ttgtggtctg
     4801 ctggcatcgc tggccgggat cattgaagtg gcgcgtctct cctccgcaca acccacggcg
     4861 gggactggct atgagctgga tgctattgct gcggtggttc tgggcggtac gagtctggcg
     4921 ggcggaaaag gtcgcattgt tgggacgttg atcggcgcat taattcttgg cttccttaat
     4981 aatggattga atttgttagg tgtttcctcc tattaccaga tgatcgtcaa agcggtggtg
     5041 attttgctgg cggtgctggt agacaacaaa aagcagtaat aacgactaca ggacatcttg
     5101 aatatgaaca tgaaaaaact ggctaccctg gtttccgctg ttgcgctaag cgccaccgtc
     5161 agtgcgaatg cgatggcaaa agacaccatc gcgctggtgg tctccacgct taacaacccg
     5221 ttctttgtat cgctgaaaga tggcgcgcag aaagaggcgg ataagcttgg ctataacctg
     5281 gtggtgctgg actcccagaa caacccggcg aaagagctgg cgaacgtgca ggacttaacc
     5341 gttcgcggca ccaaaattct gctgattaac ccgaccgact ccgacgcagt aggtaatgct
     5401 gtgaagatgg ctaaccaggc gaacatcccg gttatcactc ttgaccgcca ggcaacgaaa
     5461 agtgaagtgg tgagccacat tgcttctgat aacgtactgg gtggcaaaat cgccggtgat
     5521 tacatcgcga agaaagcggg tgaaggtgcc aaagttatcg agctgcaagg cattgctggt
     5581 acatccgcag cccgtgaacg tggcgaaggc ttccagcagg ccgttgctgc tcacaagttt
     5641 aatgttcttg ccagccagca gattttgatc gcactaaagg tttgaacgta atgcagaacc
     5701 tgttgaccgc tcatccggat gttcaggctg tattcgcgca gaatgatgaa atggcgctgg
     5761 gcgcgctgcg cgcactgcaa actgccggta aatcggatgt gatggtcgtc ggatttgacg
     5821 gtacaccgga tggcgaaaaa gcggtgaatg atggcaaact agcagcgact atcgctcagc
     5881 tacccgatca gattggcgcg aaaggcgtcg aaaccgcaga taaagtgctg aaaggcgaga
     5941 aagttcaggc taagtatccg gttgatctga aactggttgt taagcagtag ttttaatcag
     6001 gttgtatgac ctgatggtga cataaatacg tcatcgacag atgaacgtgt aatataaaga
     6061 aaagcagggc acgcgccacc ctaacacggt ggcgcatttt atggacatcc cgaatatgca
     6121 aaacgcaggc agcctcgttg ttcttggcag cattaatgct gaccacattc ttaatcttca
     6181 atcttttcct actccaggcg aaaccgtaac cggtaaccac tatcaggttg catttggcgg
     6241 caaaggcgcg aatcaggctg tggctgctgg gcgtagcggt gcgaatatcg cgtttattgc
     6301 ctgtacgggt gatgacagca ttggtgagag cgttcgccag cagctcgcca ctgataacat
     6361 tgatattact ccggtcagcg tgatcaaagg cgaatcaaca ggtgtggcgc tgatttttgt
     6421 taatggtgaa ggtgagaatg tcatcggtat tcatgccggc gctaatgctg ccctttcccc
     6481 ggcgctggtg gaagcgcaac gtgagcgtat tgccaacgcg tcagcattat taatgcagct
     6541 ggaatcacca ctcgaaagtg tgatggcagc ggcgaaaatc gcccatcaaa ataagactat
     6601 cgttgcgctt aacccggctc cggctcgcga acttcctgac gaactgctgg cgctggtgga
     6661 cattattacg ccaaacgaaa cggaagcaga aaagctcacc ggtattcgtg ttgaaaatga
     6721 tgaagatgca gcgaaggcgg cgcaggtact gcatgaaaaa ggtatccgta ctgtactgat
     6781 tactttagga agtcgtggtg tatgggctag cgtgaatggt gaaggtcagc gcgttcctgg
     6841 attccgggtg caggctgtcg ataccattgc tgccggagat acctttaacg gcgcgttaat
     6901 cacggcgttg ctggaagaaa aaccattgcc agaggcgatt cggtttgccc atgctgccgc
     6961 tgcgattgcc gtaacacgta aaggcgcaca accttccgta ccgtggcgtg aagagatcga
     7021 cgcattttta gacaggcaga ggtgacgctt ggctacaatg aaagatgttg cccgcctggc
     7081 gggcgtttct acctcaacag tttctcacgt tatcaataaa gatcgcttcg tcagtgaagc
     7141 gattaccgcc aaagttgaag cggcgattaa agaactcaat tacgcgccat cagccctggc
     7201 gcgtagcctc aaactcaatc aaacacatac cattggcatg ttgatcactg ccagtaccaa
     7261 tcctttctat tcagaactgg tgcgtggcgt tgaacgcagc tgcttcgaac gcggttatag
     7321 tctcgtcctt tgcaataccg aaggcgatga acagcggatg aatcgcaatc tggaaacgct
     7381 gatgcaaaaa cgcgttgatg gcttgctgtt actgtgcacc gaaacgcatc aaccttcgcg
     7441 tgaaatcatg caacgttatc cgacagtgcc tactgtgatg atggactggg ctccgttcga
     7501 tggcgacagc gatcttattc aggataactc gttgttgggc ggagacttag caacgcaata
     7561 tctgatcgat aaaggtcata cccgtatcgc ctgtattacc ggtccgctgg ataaaactcc
     7621 ggcgcgcctg cggttggaag gttatcgggc ggcgatgaaa cgtgcgggtc tcaacattcc
     7681 tgatggctat gaagtcactg gtgattttga atttaacggc gggtttgacg ccatgcgcca
     7741 actgttatca catccgctgc gtcctcaggc cgtctttacc ggaaatgacg ctatggctgt
     7801 tggcgtttac caggcgttat atcaggcaga gttacaggtt ccgcaggata tcgcggtgat
     7861 tggctatgac gatatcgaac tggcaagctt tatgacgcca ccattaacca ctatccacca
     7921 accgaaagat gaactggggg agctggcgat tgatgtactc atccatcgga taacccagcc
     7981 gacccttcag caacaacgat tacaacttac tccgattctg atggaacgcg gttcggctta
     8041 gatttacgct gtcttttgat caaattatta ccatcggttg ttttcagaag catgaacatt
     8101 gctgctgaag caacagtaat aatgcccatc gtgataaacg tatagtggaa ttgttcgaca
     8161 gtcgttgtgc cttccatccc ttcataaacg cgaaggacgg ccgcacttac agcaacgcct
     8221 aaactaatcg acagttgctg cgtgaccgcc agaacactgt taccgctgct ggcgttgtca
     8281 tcggtcagat cggcaagtgt gatggtattc atcgcggtaa attgcgtcga catagccatc
     8341 cctaatataa acaacggcaa gatcagcatc catatcgcca ttgccggtga ttgcaaagag
     8401 aactgagcga tcattagccc aataatcacc gtgatcccca ctaacgtatg gcgatagccc
     8461 agacgacgta agacttgggt aaccatcgat tttgcaataa tggaacctaa cgctgtcggt
     8521 gccatcatac agccggcaat aaacgcctga taaccaaatc ctacctgtaa catcaatggc
     8581 ataaggaacg atacacaacc ggtccccaga cgggttgcaa tattgcctac gataccgatt
     8641 gagaaagtgc gggttttaaa taaatctaat gaaattaatg ggtttggcgt gtgtcgcgca
     8701 tggagaatat agagaagcag taacccgatg ctggtgacaa ttaccgtcaa ggcaatccag
     8761 ctggcgacaa tcttttcccc gaatagctct attccgcttg agaagagaac aaggctgagg
     8821 ccaaacagca aaaagccagt gatatcgaat ctgcgtcgtg cggtggtgaa attgggcata
     8881 tgtttgcgcg cgtaaagaag gcccgcaata cctatgggga tattgattaa aaatatccag
     8941 tgccaggttg cccaggtgac cagcacgccg ccaagaacgg ggcctaaaat tggccccacc
     9001 agacccggca tggcgacaaa attcaatact ggaagaagtt cattacgagg ataagcgcgc
     9061 agtaaggcca gccgagcaac aggcatcatc attgcgccgc ctatcccctg aataacccgg
     9121 aagacaacca gctgtggtag cgaattagaa agtgcgcagg ccagagaacc caatgtgaac
     9181 agactcacgg caagggtaaa aatgcgacgc gtaccgaagc gatcggctag ccatccgctt
     9241 accggaataa gcatcgccac cgtcagcgta taactgatga tggctgattg catcgcgaga
     9301 ggagaacgat taaggctatg agcgattgcg ggtaaggcgg tattcagaat agtggcatca
     9361 agtgcctgca tgaagaaggc catcgccgcg atccacggca aacccgccat actgcgcttc
     9421 tttttatcgc tcattcaatg tcttgttatc gggttgtcac ttatcaggtg agcgtagcag
     9481 cgcctgacaa gctttaaatg ccgcgtcgcc atcgctttgg acaatcgcat cgacaatcgc
     9541 ctggtgcaga tttagcttta tcactgtgtc gctggtaatt gacgtgaagt aagtgtgata
     9601 aaccgaatgg aatagcgagg cgaatgaggt caaaaacgga ttggcgctca tttcatagat
     9661 atgctcatgc caggccatat cgacttcgat ccagcgttca cggcgaaagt tctcttttaa
     9721 tgccgccatt tcggccatta acgtattgag atgcgccttc tgttccgcgg tgccaaccgt
     9781 tgctgccagt aggcaggctt gcggctccag acagatacgc ataaccagaa agtgatcgat
     9841 gacctgatga aagttctctt ctgtcatcca ccaggtaagc aattcctgat caagaaaatt
     9901 ccagttcgat tgtggcatga cccgagtacc aattcgcggt cgcggtaaaa ccatcccttt
     9961 tgccgttaac gttttgaccg cttcgcgtac cgctgtacga ctcactccaa attgctcgcc
    10021 cagctcaatc tcaccaggca aaatggtgcc gggttcatat tcacctttta agatccgttg
    10081 cgccagcttc tcagccagaa catacgaaag gtttttctgt gcagctaact gttgtgcgct
    10141 taaaggcatt acttatcttc ctttttcttt ttattcctcc ttagtatgcc accaggaagt
    10201 gtgattacgg ttgcaaaaac ggcaaattgc ttgttttatg gtacattaac ggggcttttg
    10261 ctgaaaaaat gcgcggtcag aaaattattt taaatttcct cttgtcaggc cggaataact
    10321 ccctataatg cgccaccact gacacggaac aacggcaaac acgccgccgg gtcagcgggg
    10381 ttctcctgag aatctcaaca gagaaagcaa aaataaatgc ttgactctgt ggcgggaaag
//
LOCUS       DAEEKQ010000138        10342 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.139, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000138 DAEEKQ010000000
VERSION     DAEEKQ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.139"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(41..1045)
                     /gene="cra"
                     /locus_tag="KLK57_003240"
     CDS             complement(41..1045)
                     /gene="cra"
                     /locus_tag="KLK57_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HBE0893537.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREVAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1127..1213)
                     /locus_tag="KLK57_003241"
     CDS             complement(1127..1213)
                     /locus_tag="KLK57_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HBE0893538.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="KLK57_003242"
     CDS             complement(1225..1716)
                     /gene="ilvN"
                     /locus_tag="KLK57_003242"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HBE0893539.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVASTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIREVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="KLK57_003243"
     CDS             complement(1719..3443)
                     /gene="ilvI"
                     /locus_tag="KLK57_003243"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HBE0893540.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVHNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3562..3606
                     /locus_tag="KLK57_003244"
     CDS             3562..3606
                     /locus_tag="KLK57_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893541.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="KLK57_003245"
     CDS             complement(3761..4705)
                     /gene="leuO"
                     /locus_tag="KLK57_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HBE0893542.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5365..5451
                     /gene="leuL"
                     /locus_tag="KLK57_003246"
     CDS             5365..5451
                     /gene="leuL"
                     /locus_tag="KLK57_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HBE0893543.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5544..7115
                     /gene="leuA"
                     /locus_tag="KLK57_003247"
     CDS             5544..7115
                     /gene="leuA"
                     /locus_tag="KLK57_003247"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HBE0893544.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSSAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            7115..8206
                     /gene="leuB"
                     /locus_tag="KLK57_003248"
     CDS             7115..8206
                     /gene="leuB"
                     /locus_tag="KLK57_003248"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HBE0893545.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKRF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8209..9609
                     /gene="leuC"
                     /locus_tag="KLK57_003249"
     CDS             8209..9609
                     /gene="leuC"
                     /locus_tag="KLK57_003249"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HBE0893546.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFNGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKTGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVKRASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9620..10225
                     /gene="leuD"
                     /locus_tag="KLK57_003250"
     CDS             9620..10225
                     /gene="leuD"
                     /locus_tag="KLK57_003250"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HBE0893547.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
BASE COUNT         2712 a         2718 c         2523 g         2389 t
ORIGIN      
        1 ccagagggaa attcacctgg cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac
       61 cgcggcgata gagattacgt ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt
      121 ccaggcttgc caggacaatc tccagcacac gctctgcgac atcgcggtga cgttgagcca
      181 ctgccagcac cgggcactgt aagaagtcga gcagttcgtt atcgccaaag gtggcaattg
      241 ccaggtcaga aggcagttta ccatcgcgac gtagcgtgac atccatcacc ccttgcaaca
      301 acgcaaacga cgttgtgaac agcgcctgcg gcatcggatg cgtttccagc catttttcga
      361 ataactgggc agccacctcc cgctcatagc tgttggcata caggaaatgc acttcgcgcg
      421 gatcatcttt ccaggcagta cggaaacctt gttcacgcag gaagctgaca gaaagctccg
      481 gcagcgcgcc aagataaagc accgtctcgg cgggaaactt acgtaactct tccgccagca
      541 tttcggcatc atcctgatcg gcaccaacca cgctggtgaa gtgttcacga tcgagggcgc
      601 ggtccagcgc gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag
      661 gaggcaacga cgtcgaaaca ataatggcat cgacctgacg ctgtaaaagg tgctcaatac
      721 agcgcatttc gttgtctggc tgatcttctg agcaggcaat tagcagttga taaccccgtt
      781 gccgcgcctg gcgttcaaga tagttagcga tgcgggtata gctggtgttc tccagatcgg
      841 ggatcacaag accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg
      901 ggtggtaatt gtgctcacgc accacagcca tgactttttc aacggttttg tcgctcacac
      961 ggtattgctt cgctttgccg ttaataacat agcttgcagt ggtccgcgac actcccgcca
     1021 gccgagcgat ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc
     1081 attgtactgg tatgggttaa attcattaac atctaagcgc ataaaattaa ctgcggcaac
     1141 tgcttttatt ccaggttccc actgatttcg caaaaaaagc ccaacgtgac atgttgggct
     1201 gcaaattgcg cattcagatc attctcaacg cattatttta tcgccgcgcg aaagtccgac
     1261 cacaccagaa cgagcaactt ccacaatttt cgccacctcg cgaatcgatg ctaaaaatgc
     1321 atcaagctta tcgctggtgc ctgctaattg aacggtataa agcgagggtg tgacatcgat
     1381 aatttgccca cggaatattt ccgtattacg tttcacttcg tcacgcccgt aaccgctggc
     1441 ctgaattttc accagcatga tttcccgctc aacatgcgcg ccctgcccca actcactcac
     1501 gcgcaagaca tcgaccagct tgtgtaattg cttttcgatc tgctcaagta ctttttcatc
     1561 gcccacggtc tggatggtca tacgcgataa tgtcggatca tcggttgacg caacggtcag
     1621 gctttcaatg ttgtagccac gctgggaaaa aaggccaatc acgcgggata acgcgcctga
     1681 ttcattttcg agtaagactg ataatatccg gcgcataatc aggttctctc cgttttgctt
     1741 aaccacattt catccattcc gcccccgcga atctgcatcg ggtagacgtg ctcgctgcca
     1801 tcgacggtaa catcaacaaa caccaggcga ttattgtgca cctgttccag cgcctcgcta
     1861 agtttgcttt ccagctcatg cggatgagaa atctggatcc cgacgtgacc ataggcttcc
     1921 gccagacgga cgaaatcggg tagcgattgc atataagatt gtgaatgacg gccggaatag
     1981 atcatgtcct gccactgctt caccatcccc agatagcggt tattgagatt caccaccagt
     2041 acgggcaact cgtattgcaa cgcggtagac agttcctgga tgttcatctg aatactgccg
     2101 tcgccagtga cgcaaaccac ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca
     2161 ggtaaaccaa aacccatcgt gccgaggcca ccggaattga tccagcgacg cggtttgtcg
     2221 aatggataat aaagtgcagc aaacatctgg tgctgcccga catcggacgt cacgtaagcg
     2281 tctcccttcg tcaaccgcca aagagtctcg atcaccgcct gcggtttaat cttttcactg
     2341 tgagtgtcat atttcaggca ctgacgagcg cgccactgtt caatttgctg ccaccagtcg
     2401 cggatctcat ccagtggttg atgggcggat tcttgcgaca agagttcaag catttgttcg
     2461 aggacctggc gagcatcccc cacaatcggg atatccgcag tcacggtttt agaaatggaa
     2521 gtaggatcaa tatcgatatg cagaacagtg gcatttgggc agtactttgc cagattgttc
     2581 gtcgttcggt catcaaatcg taccccgacg gcgaaaatca catccgcgtt atgcatcgtc
     2641 atattggctt cgtaggtacc gtgcattccc agcatgccca gtgcctgacg atgcgttgcc
     2701 ggaaacgctc ccagccccat caatgaggaa acaacgggca gattcaacgc ctccaccgtt
     2761 tctttcaact gctgatggca gcccgccgtg attgccccac cgcctacgta gacaaccggt
     2821 ttttttgccg ctaccagcgt ttgcagagcg cgcttaattt gccctttatg tccggtagta
     2881 gtgggattgt aagaacgcat actgaccgac tccggccaga catagggtaa tttgttcgcc
     2941 ggattaagaa tatctttcgg taaatcaacg actactggtc caggacgacc acttgccgcc
     3001 agccagaaag cctttttcag cacctgcgga atgtcttccg tttgcttaac cagaaaactg
     3061 tgtttaacca ctggtcgcga aatccccacc atgtcgcact cctgaaaggc atcgtaacct
     3121 atcaacgagg tcgctacctg cccggaaagg acaactaatg gaatggaatc catataagcg
     3181 gtggcgatgc cagtaatcgc attggtcgcc cctggacccg acgttaccag cacgacgccg
     3241 acttccccgg tcgcgcgcgc cagaccatcg gccatatgca ctgccgcctg ctcatgacga
     3301 actaaaacat gatcgatacc acccacggta tgcaatgcat cgtaaatatc aaggactgcg
     3361 cctccgggat aaccgaatac ttgtttaacg ccctgatcga taagcgatcg gacgaccatc
     3421 tcggctccag acaacatctc catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa
     3481 cacattaacc gtccgatctt gcttaagcaa ttggcagcca gagacataaa cgcagggtaa
     3541 taagaataaa caggaggaaa ggtgaaaaga aacgataaaa tcctccatta cgaaggatta
     3601 aattgataat aaatcggctg aatcccacaa cttacagcaa tttctctatt tttttaatta
     3661 atcctaaaat agagagccca cacattcaga ataaaattct acattcaata aaaaatacag
     3721 aataaaccag acattcatgt ctgacctatt ctgcattcag ttagcgtttg caaattgaga
     3781 ctaattgctc ttccatccac tgatggcctt tatcgcgccc ggcagcttca tgccaggaga
     3841 gataacaggt tctgctattt tgttttaacg gcagcggtaa tacctgtaat tctaaggatt
     3901 cagcgaactc ttcagccagc caacgcggcg caatagcgac caaatgcgtt tgcgacacca
     3961 cgctaagtac gctcatcatt gccatgccct gatacgcgat actggcttgc ttatctaccg
     4021 tgtcatacca aggttgacta aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt
     4081 cgttataaac atcatgtttc agtaacgggc ccttaattgt tggatgattt ttgctggcta
     4141 ccagcaccat ttcatcttta aataatggca cgctggtaaa ttcaggacga tggaagtctt
     4201 cataactaat cacaaactcc gtttcctgat aacgcagctg atgttcagtg ttctgattta
     4261 atgaagactt gaacataaca tgtatatttg gcgcaatctg ctcaatgtga ttataaatct
     4321 gcgaggtcag aatgctgtct aacgggctgc aaacacaaag atgaaataca cgttcactgc
     4381 tcgcgggttc aaaacctgaa ccaggcaatt cattttgtac tagttgcaat gcctgacgaa
     4441 ctgagccaaa aagttgaaat gcgcgagcag tcggttgaat accacggcca taacgaacaa
     4501 aaagctcgtc attaaacatc accttcaggc gtgcaacagc gttactgacc gcaggttgcg
     4561 acatacccag aacatgagcg gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa
     4621 cggttaataa gttgagatcg accatgcgta gctgtggctt gcttaactcc gccgtctctg
     4681 aatgatctgt ttgtacctct ggcatactta actccactgt cacacttaac tccctttccc
     4741 ttattggaat gcagagaaat aatcctgaag aatatgattt atcatgcata taaaataaga
     4801 aaaagcaaaa tgagtaaaat tcggaaaaca taaagatacg ggttctgata aaaacagaat
     4861 ccattgccgt ggaatgagtc atttaccaca gcataagaat ccataatgtt tttgttgcga
     4921 aaacaatcta atcataacta ccgcgaatac tcaatcatct acaaaatgga ttaaatgtgc
     4981 ttatttaata attaatttat atatttaatg cattaattct taacattaat tgatcaataa
     5041 tattcaccaa atcaatatca aaaaaaatcg caaaacatat aattcaatac aaatcatcag
     5101 gataggtttt gcaacgcgtg cattttgtcc cctttttcct cgttgattag atgcaaaaat
     5161 ttatgctgaa atatgtcaac cgatgaaaag cgtcggtagt taagcagaaa ttaatatcgc
     5221 ttactttaac caccgcagca caattagcta attttacgga tgcagaactc acgctggcgg
     5281 gacgttttta ttgcgtcagg gttgacatcc gtttttgtat ccagtaactc taaaagcata
     5341 tcgcattcat ctggagctga tttaatgact cacatcgttc gctttatcgg tctactacta
     5401 ctaaacgcat cttctttgcg cggtagacga gtgagcggca tccagcatta agccagcacg
     5461 cagtcaaaca aaaaacccgc gccattgcgc gggttttttt atgcccgaag cgaggcgctc
     5521 taaaagagac aaggacctaa accatgagcc agcaagtcat tattttcgat accacattgc
     5581 gcgacggtga acaggcgtta caggcaagct tgagtgtgaa agaaaaactg caaattgcgc
     5641 tggcccttga gcgtatgggt gttgacgtga tggaagtcgg tttccccgtc tcttcgccgg
     5701 gcgattttga atcggtgcaa accatcgccc gccaggttaa aaacagccgc gtatgtgcgt
     5761 tagctcgctg cgtggaaaaa gatatcgacg tggcggccga atccctgaaa gtcgccgaag
     5821 ccttccgtat tcataccttt attgctacct ccccaatgca catcgccacc aagctgcgca
     5881 gcaccctgga cgaggtgatc gaacgcgcta tctatatggt gaaacgcgcc cgtaattaca
     5941 ccgatgatgt tgaattttct tgcgaagatg ccgggcgtac acccattgcc gatctggcgc
     6001 gagtggtcga agcggcgatt aatgccggtg ccaccaccat caacattccg gacaccgtgg
     6061 gctacaccat gccgtttgag ttcgccggaa tcatcagcgg cctgtatgaa cgcgtaccta
     6121 acatcgacaa agccattatt tccgtacata cccacgacga tttaggcctg gcggtcggca
     6181 actcactggc ggcggtacat gccggtgcac gccaggtgga aggtgcaatg aacgggatcg
     6241 gcgagcgtgc cggtaactgt tcgctggaag aagtcatcat ggcgattaaa gttcgtaagg
     6301 atattctcaa cgtccacacc gccattaatc accaggagat atggcgcacc agccagttag
     6361 ttagccagat ttgtaatatg ccgatcccgg caaacaaagc cattgttggc agcagcgcat
     6421 tcgctcactc ctccggtatc caccaggatg gcgtgctgaa aaaccgcgaa aactacgaaa
     6481 tcatgacacc agaatctatt ggtctgaacc aaatccagct gaatctgacc tctcgttcgg
     6541 ggcgtgcggc ggtgaaacat cgcatggatg agatggggta taaagaaagt gaatataatt
     6601 tagacaattt gtacgatgct ttcctgaagc tggcggacaa aaaaggtcag gtgtttgatt
     6661 acgatctgga ggcgctggcc ttcatcggta agcagcaaga agagccggag catttccgtc
     6721 tggattactt cagcgtgcag tctggctcta acgatatcgc caccgccgcc gtcaaactgg
     6781 cctgtggcga agaagtcaaa gcagaagccg ccaacggtaa cggtccggtc gatgccgtct
     6841 atcaggcaat taaccgcatc actgactata acgtcgaact ggtgaaatac agcctgaccg
     6901 ccaaaggtca cggtaaagat gcgctgggtc aggtggatat tgtcgccaac tacaacggtc
     6961 gccgcttcca cggcgtcggc ctggccaccg atattgtcga gtcctccgcc aaagccatgg
     7021 tgcacgtact gaacaatatc tggcgtgccg cagaagtcga aaaagagttg caacgcaaag
     7081 ctcaacacaa cgaaaacaac aaggaaaccg tgtgatgtcg aagaattacc atattgccgt
     7141 attgccgggg gacggtattg gtccggaagt gatgacccag gcgctgaaag tgctgaatgc
     7201 cgtgcgcaac cgctttgcga tgcgcatcac caccagccat tacgatgtag gcggcgcagc
     7261 cattgataac cacgggcaac cactgccgcc tgcgacggtt gaaggttgtg agcaagccga
     7321 tgccgtgctg tttggctcgg taggcggccc gaagtgggaa catttaccac cagaccagca
     7381 accagaacgc ggcgcgctgc tgcctctgcg taagcgcttc aaattattca gcaacctgcg
     7441 cccggcaaaa ctgtatcagg ggctggaagc attctgtccg ctgcgtgcag acattgccgc
     7501 taacggcttc gacatcctgt gcgtgcgcga actgaccggc ggcatctatt tcggtcagcc
     7561 aaaaggccgc gaaggtagcg gacaatatga aaaagcgttt gataccgagg tgtatcaccg
     7621 ttttgagatc gagcgtattg cccgcatcgc gtttgaatct gctcgtaaac gtcgccacaa
     7681 agtcacctca atcgacaaag ctaacgtgct gcaatcctct attttatggc gtgaaatcgt
     7741 caacgagatc gccacggaat acccggatgt cgaactggcg catatgtaca tcgacaacgc
     7801 caccatgcag ctgattaaag atccatcaca gtttgacgtt ctgctgtgct ccaacctgtt
     7861 tggcgacatt ctgtctgacg agtgcgcaat gatcactggc tcgatgggga tgttgccttc
     7921 cgccagcctg aacgagcaag gttttggtct gtatgaaccg gcaggcggct cagcaccaga
     7981 tatcgcaggc aaaaacatcg ccaacccgat tgcgcaaatt ctgtcgctgg cactgctgct
     8041 gcgctacagc ctggatgccg atgatgcggc ttgcgccatt gaacgcgcca ttaaccgcgc
     8101 attagaagaa ggcattcgca ccggggattt agcccgtggc gctgccgccg ttagtaccga
     8161 tgaaatgggc gatatcattg cccgctatgt ggcagaaggg gtgtaatcat ggctaaaacg
     8221 ttatacgaaa aattattcga cgctcacgta gtgtacgaag ccgaaaacga aaccccgctg
     8281 ttatatatcg accgccacct ggtgcatgaa gtgacctcac cgcaggcgtt caatggtctg
     8341 cgcgcccacg gtcgcccggt acgtcagccg ggcaaaacct tcgccactat ggatcacaac
     8401 gtctctaccc agacgaaaga cattaatgcc tgtggtgaaa tggcgcgcat ccagatgcag
     8461 gagctgatta aaaactgcaa agaatttggc gtcgaactgt atgacctgaa tcacccgtat
     8521 caggggatcg tccacgtaat ggggccggaa cagggcgtca ccttgccggg gatgaccatt
     8581 gtctgcggcg actcgcatac cgccacccac ggcgcgtttg gcgcactggc ctttggtatc
     8641 ggcacttccg aagttgaaca cgtactggca acgcaaaccc tgaaacaggg ccgcgcaaaa
     8701 accatgaaaa ttgaagtcca gggcaaagcc gcgccgggca ttaccgcaaa agatatcgtg
     8761 ctggcaatta tcggtaaaac cggtagcgca ggcggcaccg ggcatgtggt ggagttttgc
     8821 ggcgaagcaa tccgtgattt aagcatggaa ggtcgtatga ccctgtgcaa tatggcaatc
     8881 gaaatgggcg caaaaaccgg tctggttgca ccggacgaaa ccacctttaa ctatgtcaaa
     8941 ggccgtttgc atgcgccgaa aggcaaagat ttcgacgacg ccgttgccta ctggaaaacc
     9001 ctgcaaaccg acgaaggcgc aactttcgat accgttgtca ctctgcaagc agaagaaatt
     9061 tcaccgcagg tcacctgggg caccaatcca ggccaggtga tttccgtgaa cgacaatatt
     9121 cccgatccgg cttcgtttgc cgatccggtt aaacgcgcgt cggcagaaaa agcgctggcc
     9181 tatatggggc tgaaaccggg cattccgctg accgaagtgg ctatcgacaa agtgtttatc
     9241 ggttcctgta ccaactcgcg cattgaagat ttacgcgcgg cagcggagat cgccaaaggg
     9301 cgaaaagtcg cgccaggcgt gcaggcactg gtggttcccg gctctggccc ggtaaaagcc
     9361 caggcggaag cggaaggtct ggataaaatc tttattgaag ccggttttga atggcgcttg
     9421 cctggctgct caatgtgtct ggcgatgaac aacgaccgtc tgaatccggg cgaacgttgt
     9481 gcatccacca gcaaccgtaa ctttgaaggc cgccaggggc gcggcgggcg cacgcatctg
     9541 gtcagcccgg caatggctgc cgctgctgct gtgaccggac atttcgccga cattcgcaac
     9601 attaaataag gagcacacca tggcagagaa atttatcaaa cacacaggcc tggtggttcc
     9661 gctggatgcc gccaatgtcg ataccgatgc aatcatcccg aaacagtttt tgcagaaagt
     9721 gacccgtacg ggttttggcg cgcatctgtt taacgactgg cgttttctcg atgaaaaagg
     9781 ccaacagcca aacccggact tcgtgctgaa cttcccgcag tatcagggcg cttccatttt
     9841 gctggcacga gaaaacttcg gctgtggctc ctcgcgtgag cacgcgccct gggcattgac
     9901 cgactacggc tttaaagtgg tgattgcgcc gagttttgct gacatcttct acggcaatag
     9961 ctttaacaac cagctgctgc cggtgaaatt aagcgatgca gaagtggacg aactgtttgc
    10021 gctggtgaaa gccaatccgg ggatccattt cgacgtggat ctggaagcgc aagaggtgaa
    10081 agcgggagag aaaacctatc gctttaccat cgatgccttc cgccgccact gcatgatgaa
    10141 cggtctggac agtattgggc ttaccttgca gcacgacgac gccattgccg cttatgaagc
    10201 aaaacaacct gcgtttatga attaatcccc ttgcccggtc aaatgaccgg gctttccgct
    10261 atcgtccacg tcatcaaacg tctttaacct ttgcggttaa aaataatgcg acaacagaaa
    10321 taaccgcaat tacccagtag ac
//
LOCUS       DAEEKQ010000139        10206 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.141, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000139 DAEEKQ010000000
VERSION     DAEEKQ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.141"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            244..747
                     /locus_tag="KLK57_003251"
     CDS             244..747
                     /locus_tag="KLK57_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HBE0893548.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(826..1077)
                     /locus_tag="KLK57_003252"
     CDS             complement(826..1077)
                     /locus_tag="KLK57_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HBE0893549.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1192..1278)
                     /gene="azuC"
                     /locus_tag="KLK57_003253"
     CDS             complement(1192..1278)
                     /gene="azuC"
                     /locus_tag="KLK57_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HBE0893550.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            1541..1864
                     /locus_tag="KLK57_003254"
     CDS             1541..1864
                     /locus_tag="KLK57_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HBE0893551.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            2035..2532
                     /gene="ftnA"
                     /locus_tag="KLK57_003255"
     CDS             2035..2532
                     /gene="ftnA"
                     /locus_tag="KLK57_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HBE0893552.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(2570..2809)
                     /locus_tag="KLK57_003256"
     CDS             complement(2570..2809)
                     /locus_tag="KLK57_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HBE0893553.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSKDGFSTDQSKICRH"
     gene            3000..4211
                     /gene="tyrP"
                     /locus_tag="KLK57_003257"
     CDS             3000..4211
                     /gene="tyrP"
                     /locus_tag="KLK57_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HBE0893554.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGV
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNVRKLRWVF
                     ITGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(4273..4938)
                     /gene="yecA"
                     /locus_tag="KLK57_003258"
     CDS             complement(4273..4938)
                     /gene="yecA"
                     /locus_tag="KLK57_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HBE0893555.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTNHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(5134..5220)
                     /locus_tag="KLK57_003259"
     tRNA            complement(5134..5220)
                     /locus_tag="KLK57_003259"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5184..5186),aa:Leu,seq:taa)
     gene            complement(5233..5306)
                     /locus_tag="KLK57_003260"
     tRNA            complement(5233..5306)
                     /locus_tag="KLK57_003260"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5272..5274),aa:Cys,seq:gca)
     gene            complement(5361..5436)
                     /locus_tag="KLK57_003261"
     tRNA            complement(5361..5436)
                     /locus_tag="KLK57_003261"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5401..5403),aa:Gly,seq:gcc)
     gene            complement(5588..6136)
                     /gene="pgsA"
                     /locus_tag="KLK57_003262"
     CDS             complement(5588..6136)
                     /gene="pgsA"
                     /locus_tag="KLK57_003262"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HBE0893556.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(6193..8025)
                     /gene="uvrC"
                     /locus_tag="KLK57_003263"
     CDS             complement(6193..8025)
                     /gene="uvrC"
                     /locus_tag="KLK57_003263"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HBE0893557.1"
                     /translation="MSDQFDAKAFLKTVTCQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(8022..8678)
                     /gene="uvrY"
                     /locus_tag="KLK57_003264"
     CDS             complement(8022..8678)
                     /gene="uvrY"
                     /locus_tag="KLK57_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HBE0893558.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            8974..9150
                     /locus_tag="KLK57_003265"
     CDS             8974..9150
                     /locus_tag="KLK57_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893559.1"
                     /translation="MIKIFIGHYINAFNSIVDVTLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            9137..9361
                     /locus_tag="KLK57_003266"
     CDS             9137..9361
                     /locus_tag="KLK57_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HBE0893560.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(9428..10150)
                     /gene="sdiA"
                     /locus_tag="KLK57_003267"
     CDS             complement(9428..10150)
                     /gene="sdiA"
                     /locus_tag="KLK57_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HBE0893561.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPESWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAAHAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2578 a         2548 c         2344 g         2736 t
ORIGIN      
        1 gcactatcat taaaattcat tgcagttaca ttgatttcat caatgaaatg taaaaatata
       61 taaacttgat gatttaagca ttttcttata cccgttcaga cgttattctt atttcagatc
      121 atcgtcagaa ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa
      181 cagtaattat ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta
      241 ggtatggcaa ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac
      301 gcatccaatc tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc
      361 gccactttcc ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac
      421 tttatgaaga gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc cggtgaaaaa
      481 ctgaactctc tggaagaact gttccaaaaa acgatggaag aatacgagca acgttctagt
      541 acgttggcac agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc
      601 ctgcgcgatc tggaaaaaga acagcagcat gatggtctgt tgctgcaaac cattcttgat
      661 gaagtgcgca gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg
      721 aatgtcgtgt cacaccagct gcattgatca tcatcggcgc taatgcattg cgccgatgaa
      781 ggttttgaga aaccgctgcc tcatctgttt gaagcagcgg tttttttaat gggattcacc
      841 ctgtggggta aacttgagtt caataagcgc gatggctttt tggattgccc gcatggtgac
      901 cgggtctgcg gcggcgggat ggttagtaaa gtcgatattc ttcagctgac tggacatttt
      961 ttcacgaact tcaacgggcg cgattacatc gagaacatcc agaatttgtt tgataaccaa
     1021 ctggcaagca accacatcag aaaccagttc ctgatcggca ttcagcggct gggacatcgt
     1081 aaactcctga tagcattttg aaagccgtta tagtagcggc ttcacatctt cagcgatagt
     1141 cacatccacc gtcatcagga cacaaaaaaa cctgccggag caggtttttt gttatcggaa
     1201 catattgcct ggcggtacgt ctttgaacgt cttgcaatag ttattgaaca tacttttcag
     1261 gattttgcgc agtttcatcg cggcactccg accatttgtt atacaggtgt tattgtcttt
     1321 gcgcttataa tatgacaacc atcacaaaaa tcaatcttta tgtgatacaa atcacataaa
     1381 taccccttta atgttataaa aatgataatc aaaaaacagc ccccctattt ctgacaccta
     1441 cagatggcaa gaaatagcgc ctgccaggcg tcttttccgg ccattgtcgc agcgctgtaa
     1501 cgcgtaaaat agtgctttct cttactcttc tggctggacc atgagacttc tgattctgac
     1561 tctttcatta ataacgctgg cggggtgtac ggttactcgc caagcccatg tgagcgaagt
     1621 tgacgcggca acaggtattg tacggttggt ttatgaccag gcttttttgc agcatgccca
     1681 taccgatcgt tatgtcagtc gcgggatcgc cgatcgcgca tgccagcagg aaggctatac
     1741 ccacgcggtc ccctttggtc agccagtagg caactgtagc ctttttgcgg gttccttatg
     1801 tttgaatacg gaattcactt tgtcatatca gtgccatcat tccgccttcc ctgtctttct
     1861 ctaaatattt taatcagcga ggggatcttc gctgattaaa gaaatagcgg taatgcgttt
     1921 tattcccatt cgtattttta ataattgaag tttatatttt accttttgca aataataaaa
     1981 taacaaatta tagtgacgcc acagcaacaa atataacctt tgtggagcac tatcatgctg
     2041 aaaccagaaa tgattgaaaa acttaatgag cagatgaacc tggaactgta ctcttcactg
     2101 ctttatcagc aaatgagcgc ctggtgcagc tatcatacct tcgaaggtgc tgccgcgttc
     2161 ctgcgccgtc acgcccagga agagatgacg catatgcagc gtctgtttga ttacctgact
     2221 gataccggca atttaccgcg tattaatacc gttgaatctc cgtttgctga atattcctca
     2281 cttgatgaat tattccagga aacctataaa cacgaacaat taatcaccca gaaaattaac
     2341 gaactggctc atgctgcaat gaccaatcag gactacccaa catttaattt cctgcagtgg
     2401 tatgtttctg agcagcatga agaagagaaa ctgttcaaat cgattattga taaattaagc
     2461 ctggcaggca aaagcggcga aggtctgtat tttatcgaca aagaactctc taccctcgac
     2521 acacaaaact aatgctctcc ggcggcaggt ttactgccgc cgtttcaaat cagtgacggc
     2581 aaatcttact ctgatcggtc gaaaaaccgt ctttcgaagg tataaatttg ccttttgctg
     2641 ccagaaacgc taccagctct cccgctgtca tcccttctgc cgagcaggtg tgaaaacgtg
     2701 cctgttcacc aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc
     2761 ctgattcaat catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc
     2821 aaaaaaagcc tagcgtagcg attgccgctt atgaagactt tgcgccagcg caggactgaa
     2881 tgctttttat tgtacattta tatttacact atatgtaacg tcggtttgac gaagcagccg
     2941 ttatgcctta acctgcgccg cagatatcac tcataaagat cgtcaggaca gaagaaagcg
     3001 tgaaaaacag aaccctggga agtgttttta tcgtggcggg aaccacaatt ggcgcaggca
     3061 tgctggcaat gccgctggct gcggccggtg ttggttttag cgttacgtta atcttgttga
     3121 ttgggctttg ggcgttgatg tgctacacgg cgctattact gctggaggtg taccagcatg
     3181 ttccggcaga taccggtctg ggcacgctgg caaaacgcta tctgggacgc tacggtcaat
     3241 ggctgacggg cttcagtatg atgttcttaa tgtatgctct gactgcggca tacatcagcg
     3301 gtgtcggtga attgttggcc tccagcatca gcgactggac aggtatttca atgtcggcaa
     3361 ccgctggcgt gctgttgttc acttttgttg ccggtggcgt ggtttgtgtt ggaacatcgc
     3421 tggtcgattt atttaaccgt tttctgttca gcgccaaaat tatttttctg gtggtgatgc
     3481 tggtattgct gctaccgcat attcacaaag tgaatctttt aaccctgccg ttgcaacagg
     3541 ggctggctct gtcagcaatc ccggtgattt ttacctcatt tggttttcac ggtagcgtgc
     3601 cgagtattgt cagctatatg gatggcaacg ttcgtaagct acgctgggtg tttataaccg
     3661 gtagtgcgat ccccctggtg gcatatattt tctggcaggt ggcaacgctt ggcagcattg
     3721 attcaacaac ctttatggga ttgctggcta atcatgctgg attaaacggg ctgttacagg
     3781 cgttacgcga aatggtggcc tctccgcatg ttgagctggc agtgcattta tttgctgatt
     3841 tagccctcgc cacgtcattt ctcggcgttg cgttaggctt atttgattat ctggctgatt
     3901 tatttcagcg ttcaaatacc gttggtggac ggttgcaaac tggtgcaatt acctttctgc
     3961 cgccgttggc gtttgcactg ttttatccac gaggatttgt gatggcgctg ggttacgccg
     4021 gtgtggcgct ggcggtactg gcattgatta tcccttcgct gttgacctgg caaagcagaa
     4081 agcacaatcc tcaggcgggt taccgggtca aaggtggtcg tccggcgctg gtggtggtgt
     4141 ttctctgtgg tattgctgtg attggcgtgc aatttttgat tgcggcaggg ttgttaccag
     4201 aagtggggtg atcagatagc ctcaaattcc ttattgggtg ccagaattaa cgctgacacc
     4261 caatttggcc tcttaatgca ggcagcactg cttaaatttc ttaccactac cgcacgggca
     4321 aggatcgtta cgccccggtt tctcttctgc tttgatcggt tgctgaacag ctttttcctg
     4381 cggatgcgcc atccagtacg catgtagatc aagcgccgcc agtcgaatgg catctacgct
     4441 ctcttcaaac gcttctggcg acatcttttc tacccgctcg aagttttcct cagtaccgtg
     4501 cagcgcaatc gcctccagcg ctggttttaa cgaatcgggc aacgttgacc agtcagaaag
     4561 tgccacgccc cgcatatagc caaagcacca ctcctcaaca atcgtcagct cgctgccatc
     4621 aacttctcgc aagccgaata acggctcaaa ctgctccggg aattcgttca gacgctctgc
     4681 ggtatcggcc atatgttgaa aagccaggtt cataaagcgc gtcatctctt tctctgacgc
     4741 ccagcgcggc acatagtcag ccccacccca cacggcaacc agccactgtt ccggttcaat
     4801 ctcttgcgga gaactcaaca ccgccgtcaa taaaccgtcc agctccgcca catcaaggat
     4861 ggcgtggtta gtgttgtatt tggtcagaat atcgtccagc cattccaact cactttcgtt
     4921 taacggtccc gttttcatac gcttttcctt gtggctctca actcgccagc acctatctta
     4981 catgccggtc cgtatcagag atactttttg agtggctttg ccggtgatta aaaattaagg
     5041 agggtgtaac gacaagttgc aggcacaaaa aaaccacccg aaggtggttt cacgacactg
     5101 cttattgctt tgattttatt cttatctttc ccatggtacc cggagcggga cttgaacccg
     5161 cacagcgcga acgccgaggg attttaaatc ccttgtgtct accgattcca ccatccgggc
     5221 tcgggaagaa agtggaggcg cgttccggag tcgaaccgga ctagacggat ttgcaatccg
     5281 ctacataacc gctttgttaa cgcgccaaat tcttcaggcc tttcagccag acatccgctt
     5341 gacgccgatg tcttttaaac tggagcggga aacgagactc gaactcgcga ccccgacctt
     5401 ggcaaggtcg tgctctacca actgagctat tcccgcattc atcaagcaat cagttaatca
     5461 cttgatttta ttatcgtctg gcaatcagtg ccgccgttcg atgcgttgca ttctacttac
     5521 ctggcgcgat gagtcaacga tatttttcac cacttttgat cgtttgctga aaattacgcc
     5581 gaaacgatca ctgatcaagc aaatctgcac gcgcagcgct caaatattgc aacattgacc
     5641 acagagtcag taccgcagcc acaaagaaaa gtgcaatacc ggcgtactca acccaaatgt
     5701 tcggacgcca cagcagccat gccaacgcca ccatctgggc agtggttttc actttcccaa
     5761 tccaggagac agccacgcta ctgcgtttac ccaactccgc catccattcg cgtagcgcag
     5821 aaataataat ttcacgggcg atcatcgttg ctgccggtaa ggtcacccac cagctgtggt
     5881 aatgctcggt taccagcacc atggcgatag ccacgagaac tttatctgct acagggtcaa
     5941 ggaaagcacc aaaccgggta ctctggttcc agcggcgtgc cagaaaacca tcgaaccagt
     6001 cagtcaccgc cgcgacgcag aaaatgagcg cggcggcaaa cggcgaccag gtgacaggca
     6061 gataaaagac caatacaaag aatgggataa ggatgacacg gaacagtgta agcaacgtag
     6121 ggatattaaa ttgcataatg acgggtaact atctgttgtc agtaagatta cccctatgtt
     6181 gctacagaga catcaatgtt tcaacgacca gaagatcttt tctgccagac cttgcgaaat
     6241 acccggcact tttgcaattt cctcgacgct ggcgttacgt aaaccttgca aaccgcccat
     6301 atatttcaac aacatttgcc gacgttttgg cccgacgcct tcaatggttt ccagggaact
     6361 ggtatttttg accttcgccc gttttttacg gtgcccgcca atcgcgtgat catgtgattc
     6421 atcgcgaata tgctggataa catgcagcgc gggagaatct ggcggcaaac tgaatccctc
     6481 accttccggc tcaaagaaca gcgtttccag cccagcctta cgatctgctc ctttggcaac
     6541 gccaagtagc agcggatgat ttttatccca tgagacatcc agttcggcga agacattttt
     6601 cgcctgcgca agctggcctt tgccgccgtc gataaggatc acatccggga tcttactgtc
     6661 gtcaatggct ttaccataac gccgacgcag cacctgattc atcgccgcat aatcatcgcc
     6721 cggcgtgatg cctgtaatgt tatagcgccg atactccgca cgcagcgggc cgttagcatc
     6781 aaacaccaca caggaagcga cggtttgttc gcccatggta tggctgatgt caaagcactc
     6841 catccgcttc acttccggca atttcaacac gctggcaagc gcggtcagtc gctggtgaac
     6901 ggtagattgc tgcgaaagtt tgctggttaa ggccgtcgcc gcattggtgc gcgcgagttt
     6961 cagataacgc gccctatcgc cgcgaggttt ggtttgaaca ttaatcttgc gtcccgccag
     7021 ttctgaaagg gaatcggcga gcagcgtttt atcgctaaga ttaaaatcga gcaggatctc
     7081 acccggtaag gtgcgcatct ggctgccttg taaatagaac tggcctacga aggtttctac
     7141 cacctcgctc agttccgtac cgccaggcac tttcgggaaa tagctgcggc tgccgagcac
     7201 tttgccctga cgaatgaaca atacgtggac acaagccatg cccgcatcga acgccacacc
     7261 aataacgtcg aggtcgtcgc cggtattgga aacgaattgt ttttcggtga cgcgtcgcac
     7321 cgcctgaatt tggtcgcgaa tacgtgcagc ttcttcaaac tccagattct ggctggcagt
     7381 ttccatacga ctaatgagtt gcgtaagcac ctgatcatct ttgccagaca aaaacaggcg
     7441 cacatactca acctgctgag cgtattcttc ttcactcacc agtccttcaa cgcacggtcc
     7501 cagacagcgc cctatctgat attgcagaca cggacgcgag cgattgcgat aaacactatt
     7561 ttcgcactgg cgaatgggga aaatcttttg cagtagcgcc agtgtttcac gtacggcata
     7621 gccattcggg aacgggccga aatattcacc tttggcatgc ttcgcaccac gatgcatcgc
     7681 cagacgcggg tgggtatcgc cactcaggaa gataaaagga taagatttat catcgcgtag
     7741 caaaacgttg taacgcggct gatagagttt gatgtagttg tgttccagca acagcgcttc
     7801 ggtttctgta tgcgtaaccg ttacatcaat ttgctggatc tgggcgacca gcgcttcggt
     7861 tttgcgcgaa gcgaggttgc tgcggaaata gctggaaagc cgttttttca ggtctttcgc
     7921 tttgccgaca tagataaccg taccaccagc atcgtacatg cgataaacgc ctggctggca
     7981 ggttacggtt tttaaaaacg cttttgcgtc aaactgatca ctcactgact tgataatgtc
     8041 tccgcattac acagaccatg gcgaattgcc aggtgagtca gctcaacatc gccatgaatg
     8101 tttagtttac tgaacatacg atagcggtag ctgttcaccg ttttcggact gagattgagc
     8161 tgttctgaga tctcattgac cttctggccc ttggtgatca tcagcataat ctgcaattca
     8221 cgttcagaca aactggcaaa tgggctttct gttttttctg gttcgatctg gcttaacgcc
     8281 atttgttgag cgatatcaga agcaatgtaa cgctgccctg aatagacaga acgaatcgca
     8341 ctcacgactt cctgcggagc cgcgcctttg ctgaggtagc ccgcagcacc ggcctgcatg
     8401 actttcgctg gtaaagggtt ttctgtatgg acggtaagca tgatgatttt gacatcagct
     8461 gtggaacgcg cgattttacg cgtcgcctca agaccgccaa tgcccggcat actcatgtcc
     8521 attagcacca cgtcaacggc atttgtccgg caccacttaa cggcgtcttc accgcacgat
     8581 gcctcaccga cgacttttat accctttata tcttccagaa tgcgtcgtat ccctgcgcgc
     8641 accagttcgt ggtcatcaac aagtagaacg ttgatcaaag gaatatctcc agaaataggg
     8701 ataacgctac tgatagttag tcattcgtat attagcggtt tttattgcaa ctttgaaacg
     8761 ttaaaaatgg tgggtattcg atttttctct cgtttttgta aattcgattg tccacagtta
     8821 tggtcacgcc cgtcaccaac ggcttccggg atcgtttcac agtaagcccg gcgatgaaat
     8881 gtgacaaaaa ttgactttat tcagcaaaaa tgaaaatcag cccgctgaat atgtaacatt
     8941 aattaacccg aacaatacgg gcaaaaaaca ttcatgataa aaatatttat tggtcattat
     9001 attaacgcat ttaatagcat tgttgacgtc acgctcaaaa aacaaccact gctattttta
     9061 gcaaagctta tggtatactc cgccgcctta acatttttca ccgcaaattt tcattgcaac
     9121 atgacgagga aaataaatga gtacgcctga tttttctact gccgagaata atcaagaact
     9181 ggcaaatgaa gtctcctgcc tgaaagcgat gctgacgctg atgctacagg cgatgggaca
     9241 agctgacgcg ggccgcgtga tgttaaagat ggaaaaacag cttgcgctga tcgaagacga
     9301 aacccaggct gcagtatttt ccaaaacggt taagcaaatt aaacaagcct accgtcagta
     9361 atataaaacc ggctgagagc gtgcctttca gccgattttt gcatctggca cgcagaaaga
     9421 aaagcgatca aattaagcca gtagcggccg cgtaacaggc cacctgggtc ttatttggtg
     9481 cattgatttt tttctgcatg tttttctgat ggaaattgac cgtattctca gagattgaca
     9541 aaatcatcgc tatctctgct gatgtcttcc cttccgccgt ccatttcaga atttcttttt
     9601 cgcgcttgct gaaattcatc tctggcgtca tcactatttc atcatttaaa cgcatcagag
     9661 ccatcagact ttcgcgcacc agtaactgca tttttaattg cagttcatca ctaagaatgg
     9721 gtatttcgcg cgtgctgcaa cgggaaaagg acaaaaagcc cagcgcccga tttggcagca
     9781 ttaaatactg agtgacaccg cggcgtaaac catgtgcgtg cgcggcttcc cataatggct
     9841 gtgcttcgct gaataagtca tcattccaca ttaaatggcc ctgactaaag ttttcagggt
     9901 tcagcacagg atcaattgcg agaaaatttt ttgcctgata ataactaacc cacgactcag
     9961 ggtaattggt gtagaaagcc actttaggtc gcgtgaatgg taccgggtgg cgaacacata
    10021 acgaatagta atcgtactcc agctgctgag cctgaagctc tatttcatgg tagacctctt
    10081 ctgcggcctc catcctctga aaacgcaaca gcatcgtgcg acgccagctg aaaaaatccg
    10141 tatcctgcat agtaaaccgc aacgcccctg agagtgagta tcatttataa tgataattca
    10201 aactat
//
LOCUS       DAEEKQ010000140         9851 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.142, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000140 DAEEKQ010000000
VERSION     DAEEKQ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.142"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(382..1359)
                     /gene="epmA"
                     /locus_tag="KLK57_003268"
     CDS             complement(382..1359)
                     /gene="epmA"
                     /locus_tag="KLK57_003268"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HBE0893562.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1684..3492
                     /gene="frdA"
                     /locus_tag="KLK57_003269"
     CDS             1684..3492
                     /gene="frdA"
                     /locus_tag="KLK57_003269"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HBE0893563.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQECFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            3485..4219
                     /gene="frdB"
                     /locus_tag="KLK57_003270"
     CDS             3485..4219
                     /gene="frdB"
                     /locus_tag="KLK57_003270"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HBE0893564.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALVYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4230..4625
                     /gene="frdC"
                     /locus_tag="KLK57_003271"
     CDS             4230..4625
                     /gene="frdC"
                     /locus_tag="KLK57_003271"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HBE0893565.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4636..4995
                     /gene="frdD"
                     /locus_tag="KLK57_003272"
     CDS             4636..4995
                     /gene="frdD"
                     /locus_tag="KLK57_003272"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HBE0893566.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            5058..6191
                     /locus_tag="KLK57_003273"
     CDS             5058..6191
                     /locus_tag="KLK57_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBE0893567.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6280..6813
                     /gene="blc"
                     /locus_tag="KLK57_003274"
     CDS             6280..6813
                     /gene="blc"
                     /locus_tag="KLK57_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBE0893568.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="KLK57_003275"
     CDS             complement(6810..7127)
                     /gene="sugE"
                     /locus_tag="KLK57_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBE0893569.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="KLK57_003276"
     CDS             complement(7302..7448)
                     /gene="ecnB"
                     /locus_tag="KLK57_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBE0893570.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="KLK57_003277"
     CDS             complement(7559..7684)
                     /gene="ecnA"
                     /locus_tag="KLK57_003277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBE0893571.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(7736..8302)
                     /gene="efp"
                     /locus_tag="KLK57_003278"
     CDS             complement(7736..8302)
                     /gene="efp"
                     /locus_tag="KLK57_003278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBE0893572.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8344..9372
                     /gene="epmB"
                     /locus_tag="KLK57_003279"
     CDS             8344..9372
                     /gene="epmB"
                     /locus_tag="KLK57_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBE0893573.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
     gene            9762..>9851
                     /locus_tag="KLK57_003280"
     CDS             9762..>9851
                     /locus_tag="KLK57_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893574.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPR"
BASE COUNT         2517 a         2504 c         2502 g         2328 t
ORIGIN      
        1 aggcgaatgg aataaagaat aacaatgcag aaaatatata aaagggaatc gcactataac
       61 ccattaagta ataagccgat gggctattgg caaatccaaa tacggaagta aagatcatca
      121 atatgagtcc tatcagactc atctttttta tcgtgtgagg catgaaacca tccttcattt
      181 cagcgcgtgt ataacacgtg cccactgaac tgcactgcgc aacattgagc aggcagcgta
      241 aaaaccaata cagaattgat acccggatga tatcaaatat ccagtcttaa attgtggcct
      301 ttaacggaaa aatgaaaaca tatgctacag aatggcgctt atcacgccat tcttcgctgt
      361 taattcagta atttttcaga attatgcccg gtcaacgcta aaggcgatga cttcagccag
      421 tgtctccgcg cccagcgcca acatcaccag acgatcaaca cctaatgcca cgccggaaca
      481 gtcaggcata ccgactttca aggcttcaat cagattctgg tcaatggggt gctgcggcaa
      541 accgcgcgcc gcgcgcttac ggttatcttg ttcaaagcgt tgttgctgct cacgggcatc
      601 cgttaattca tggaaaccat tcgccagctc aataccttta taataaacct caaagcgttc
      661 agcgacccga tgatcttcgg tactgatttg cgccagtgat gcctggctgg ctggaaagtg
      721 gtacacaaag gtcggttttt ctttgccaat atttggctct acgccaaagg taaacagcaa
      781 ttgtagcaac gtgtcgcggt cttcttcggt atcagcaacg ttactcaaat ccagtttcgc
      841 tgcgacttcc cgcagttgcg ttttgtcggc agagagcggg tcaatttcca gataacgcaa
      901 gaaagcttgt tgataagaaa ggctttctgc tgccgggcag tccagcacct gttgtaagag
      961 atcgtccacc tcgttcatca accggtacat atcatagtgc ggtcgatacc actcaagcat
     1021 agtgaactca gggttgtgat aacgccccat ctcttcatta cggaagctgc ggcacagctg
     1081 gaataccggc ccacaaccgg caaccagcag gcgtttcata tggtattccg ggctggtcat
     1141 taaccagaga ttcatcccct gcgaatgccc ggggccaacg aaacgtgtct caaacgggac
     1201 caaatgaata tcggttaccg tcgcctggct catacaaggc gtctccacct ccagcactcc
     1261 acgatcggca aagaaacgac ggatctccgc cataatcgcc gcacgtttta ataagttagg
     1321 aatggatgcg ctcggctgcc aggatgccgt ttcgctcata gttaaatctc cagtttttga
     1381 caagggcacg aagtctactc gcaacgcgac ggcgagacaa attttacgca ggaatcaaac
     1441 agcggtgggc agtgactaaa aaaagcacga tctgatggtt tagtaattaa attaatcatc
     1501 ttcagtgata atttagccct cttgcgcact aaaaaaatcg atctcgtcaa atttcagact
     1561 tatccatcag actatactgt tgtacctata aaggagcagt ggaatagcgt tcgcagaccg
     1621 taactttcag gcacttaccc tgaagtacgg ggctgtggga taaaaacaat ctggaggaat
     1681 gtcgtgcaaa cctttcaagc cgatcttgcc attgtaggcg ccggtggcgc gggattacgt
     1741 gctgcaattg ctgccgcgca ggcaaatcca aatgcaaaaa tcgcactaat ctcaaaagta
     1801 tacccgatgc gtagccatac cgttgctgca gaagggggct ccgccgctgt cgcgcaggat
     1861 catgacagct tcgaatatca ctttcacgat acagtagcgg gtggcgactg gttgtgtgag
     1921 caggatgtcg tggattattt cgtccaccac tgcccaaccg aaatgaccca actggaactg
     1981 tgggggtgcc catggagccg tcgcccggat ggtagcgtca acgtacgtcg cttcggcggc
     2041 atgaaaatcg agcgcacctg gttcgccgcc gataagaccg gcttccatat gctgcacacg
     2101 ctgttccaga cctctctgca attcccgcag atccagcgtt ttgacgaaca tttcgtgctg
     2161 gatattctgg ttgatgatgg tcatgttcgc ggcctggtag caatgaacat gatggaaggc
     2221 acgctggtgc agatccgtgc taacgcggtc gttatggcta ctggcggtgc gggtcgcgtt
     2281 tatcgttaca acaccaacgg cggcatcgtt accggtgacg gtatgggtat ggcgctaagc
     2341 cacggcgttc cgctgcgtga catggaattc gttcagtatc acccaaccgg tctgccaggt
     2401 tccggtatcc tgatgaccga aggctgccgt ggtgaaggcg gtattctggt caacaaaaat
     2461 ggctaccgtt atctgcaaga ttacggcatg ggcccggaaa ctccgctggg cgagccgaaa
     2521 aacaaatata tggaactggg tccacgcgac aaagtttctc aggccttctg gcacgaatgg
     2581 cgtaaaggca acaccatctc cacgccgcgt ggcgatgtgg tttatctcga cttgcgtcac
     2641 ctcggcgaga aaaaactgca tgaacgtctg ccgttcatct gcgaactggc gaaagcgtac
     2701 gttggcgtcg atccggttaa agaaccgatt ccggtacgtc cgaccgcaca ctacaccatg
     2761 ggcggtatcg aaaccgatca gaactgtgaa acccgcatta aaggtctgtt cgccgtgggt
     2821 gaatgttcct ctgttggtct gcacggtgca aaccgtctgg gctccaactc cctggcggaa
     2881 ctggtggtct tcggccgtct ggccggtgaa caagcgacag agcgtgcagc aactgccggt
     2941 aatggcaacg aagcggcaat tgaagcgcag gcagctggcg ttgaacaacg tctgaaagat
     3001 ctggttaacc aggatggcgg cgaaaactgg gcgaagatcc gcgacgaaat gggcctggca
     3061 atggaagaag gttgcggtat ctaccgtacg ccggaactga tgcagaaaac catcgacaag
     3121 ctggcagagc tgcaggaatg cttcaagcgc gtgcgcatca ccgacacctc cagcgtgttc
     3181 aacaccgacc tgctctacac cattgaactg ggccacggtc tgaacgttgc tgaatgtatg
     3241 gcgcactccg caatggcacg taaagagtcc cgcggcgcac accagcgtct ggacgaaggt
     3301 tgcaccgagc gtgacgacgt caacttcctc aaacacaccc tcgccttccg cgatgctgat
     3361 ggcacgactc gcctagagta cagcgacgtg aagattacta cgctgccgcc agctaaacgc
     3421 gtttacggtg gcgaagcgga tgcagccgat aaggcggaag cagccaataa gaaggagaag
     3481 gcgaatggct gagatgaaaa acctgaaaat tgaggtggtg cgctataacc cggaagtcga
     3541 taccgcaccg catagcgcat tctatgaagt gccttatgac gcaactacct cattactgga
     3601 tgcgctggtc tacatcaaag acaacctggc accggacctg agttaccgct ggtcctgccg
     3661 tatggcgatt tgtggttcct gcggcatgat ggttaacaac gtgccaaaac tggcatgtaa
     3721 aaccttcctg cgtgattaca ccgacggtat gaaggttgaa gcgttagcta acttcccgat
     3781 tgaacgcgat ctggtggtcg atatgaccca cttcatcgaa agtctggaag cgatcaaacc
     3841 gtacatcatc ggcaactccc gcaccgcgga tcagggtact aacatccaga ccccggcgca
     3901 gatggcaaag tatcaccagt tctccggttg catcaactgt ggtctgtgct acgccgcgtg
     3961 cccgcagttt ggcctgaacc cagagttcat cggtccggct gccattacgc tggcgcatcg
     4021 ttataacgaa gatagccgcg accacggtaa gaaggagcgt atggcgcagt tgaacagcca
     4081 gaacggcgta tggagctgta ctttcgtggg ctattgctcc gaagtctgcc cgaaacacgt
     4141 cgatccggct gcggccattc agcagggcaa agtagaaagt tcgaaagact ttcttatcgc
     4201 gaccctgaaa ccacgctaag gagtgcaaca tgacgactaa acgtaaaccg tatgtacggc
     4261 caatgacgtc cacctggtgg aaaaaattgc cgttttatcg cttttacatg ctgcgcgaag
     4321 gcacggcggt tccggctgtg tggttcagca ttgaactgat tttcgggctg tttgccctga
     4381 aaaatggccc ggaagcctgg gcgggattcg tcgacttttt acaaaacccg gttatcgtga
     4441 tcattaacct gatcactctg gcggcagctc tgctgcacac caaaacctgg tttgaactgg
     4501 caccgaaagc ggccaatatc attgtaaaag acgaaaaaat gggaccagag ccaattatca
     4561 aaagtctctg ggcggtaact gtggttgcca ccatcgtaat cctgtttgtt gccctgtact
     4621 ggtaaggagc ctgagatgat taatccaaat ccaaagcgtt ctgacgaacc ggtattctgg
     4681 ggcctcttcg gggccggtgg tatgtggagc gcaattattg cgccggtgat gatcctgctg
     4741 gtgggtattc tgctgccact ggggctgttt ccgggtgatg cgctgagcta cgagcgcgtt
     4801 ctggcgttcg cgcagagctt cattggtcgc gtattcctgt tcctgatgat cgttctgccg
     4861 ctgtggtgtg gtttacaccg tatgcaccac gcgatgcacg atctgaaaat tcacgtgcct
     4921 gcgggcaaat gggttttcta cggtctggct gctatcctga cagttgtcac gctgattggt
     4981 atcgttacaa tctaacgtat cgccaatgta aatccggccc gcctatggcg ggccgttttg
     5041 tatggaaacc agaccttatg ttcaaaacga cgctctgcgc cttattaatt accgcctctt
     5101 gctccacatt tgctgcccct caacaaatca acgatattgt gcatcgcaca attaccccgc
     5161 ttatagagca acaaaagatc cccggtatgg cggtggcggt aatttatcag ggtaaacctt
     5221 attactttac ctggggctat gcggacatcg ccaaaaagca gcccgtcaca cagcaaacgt
     5281 tgtttgagtt aggttcggtc agcaaaacat ttacgggcgt gcttggtggc gacgctattg
     5341 ctcgagggga aatcaagtta agcgatcccg caacaaaata ctggcctgaa cttactgcta
     5401 aacagtggaa tgggatcaca ctattacatc ttgcgaccta caccgctggc ggcctgccat
     5461 tgcaggtgcc ggatgaagtg aaatcctcaa gcgacttgct gcgcttctat caaaactggc
     5521 agcctgcatg ggctccagga acacaacgtc tgtatgccaa ctccagtatc ggtttgttcg
     5581 gcgcactggc tgtgaagccg tctggtttga gttttgagca ggcgatgcaa actcgtgtct
     5641 tccagccact caaactcaac catacgtgga ttaatgtacc tcccccagaa gaaaagaatt
     5701 acgcctgggg atatcgcgaa ggtaaggcag tgcatgtttc gccaggggcg ttagatgctg
     5761 aagcttatgg tgtgaagtcg accattgaag atatggcccg ctgggtgcaa agcaatttaa
     5821 ccccccttga tatcaatgag aaaacgcttc aacaagggat acaactggca caatctcgct
     5881 actggcaaac cggcgatatg tatcagggcc tgggctggga aatgctggac tggccggtca
     5941 atcctgacag catcattaac ggcagtgaca ataaaattgc actggcagca cgcccggtaa
     6001 aagcgattaa gcccccaact cctgcagtac gcgcatcatg ggtacataaa acgggggcga
     6061 ccggcggatt tggtagctat gtcgcgttta ttccagaaaa agagctgggt atcgtgatgc
     6121 tggctaacaa aaactatccc aatccagcga gagtcgccgc cgcctgtcag attctcaacg
     6181 ctctacagta aaatttcatc gggtccgaat tttcggacct tttctgcgct tttccttgct
     6241 gtcatctaca cttagaaaaa aaccagtaag gaaacattta tgcgcctgct ccctctcgtt
     6301 gccgcagcga cagctgcatt tctggtcgtt gcctgcagtt ctcctacgcc gccgcgtggc
     6361 gtgacagtag tgaataattt cgacgctaaa cgctatctcg gtacctggta tgagattgcc
     6421 cgttttgatc accgttttga acgtggactg gaaaaagtca ccgcaacata cagtctgcgt
     6481 gatgacggcg gcctgaatgt catcaataaa ggctataacc ctgacagaga aatgtggcag
     6541 cagagtgaag ggaaagcgta ctttaccggc gcaccaacac gcgctgcgct gaaagtgtca
     6601 ttctttggtc ctttctatgg cggttataac gtcattgcac ttgatcggga ataccgccat
     6661 gcgctggttt gcggcccgga ccgcgactac ctgtggatac tctcccgcac gccaaccatt
     6721 tctgacgaag tgaaacagga gatgctggca gtcgcgaccc gggaagggtt tgatgtcagt
     6781 aagtttattt gggtacagca gcccggtagt tagtgagtgc tgagtttcag accaataatc
     6841 cccaatacga ttaacgccag actcgccagg cgcatcgggt tagcggactc accgagcagc
     6901 acaatgccgg tgatggccgc gccgactgcg ccaatacccg tccacacggc ataagccgtc
     6961 cctaccggta acgatttcat cgcccaggca agtagcgcca tactgactat catcgccgtc
     7021 acagtaataa cacttggcgt caaacgacta aagccgtggg tatatttcag gccaacggcc
     7081 cataccactt ccagcagacc agcaataact aagataatcc aggacatatc aggctccgga
     7141 gaattggggc catccccggt gaaaagaggc gtttgcaggt cgtcctgcaa agctgatggt
     7201 tgaacggata ttttgccagg tggcagggga atttcaacta ttacctttgt gactatcgca
     7261 aaaaaacaaa cggcacgaca taactgtcgt accgttattg cttattgctg cgctttcgtt
     7321 gctgcaccag aaatcgcgtt accgccatca gaaatgtctt caccaacgcc acgcgtggtg
     7381 ttgcaggcag ttaatactgt tgaaagcacc agaacagaaa agatcgctgc aattgtcttc
     7441 ttcaccataa cgtcttcctt ttaggtaatg ttatttatgt ttgcctatag caaattaata
     7501 atagacaaca tcgccaaaaa tgatggaaat ctgatgattt ttcggaagag aagaaaaatt
     7561 agctggcagc gcgggagatg gagttgccga gatgtttgat gtcttcgccg aaaccgcgag
     7621 cggtgttaca gcccgtgagc agcgtgctgg caagcaaaac aagaacgata aggcgtttca
     7681 tcatctctga cctgaataag tgatggtgca gcctgcaggc cgcaccacaa ccgcattact
     7741 tcacgcgaga gacgtattca ccagagcggg tatccacttt gatgacttcg ccgatttgta
     7801 cgaacagcgg aactttaacc acagcgccag tagacagggt agccggtttg ccgccagtac
     7861 ctgcggtatc acctttcagg cccggatcgg tatcaacgat ttccagttca acgaagttcg
     7921 gcggagtaac ggagatcggc tgaccattcc acagagttac gatacactct gcctgatcca
     7981 gcagccattt agcgttgtcg ccaattgctt ttgcatcagc agacagctgc tcgaaagttt
     8041 cgttgttcat gaagtgccag aactcaccgt cgttgtacag gtaagtcagg ttcatatcga
     8101 caacatcagc gccttcagcg gaatcagtag atttgaaggt tttttctacg cgagtaccgg
     8161 tcagcagacg acgcagttta acgcgagcaa atgcctggcc tttacccggt tttacgaatt
     8221 cactcgcttc aaccgcgtaa ggttcgccgt ctaacatgat tttaagacca gcacgaaaat
     8281 cgttgctata gtacgttgcc ataaggccct ctgaaatttg ttaattggta gctaagccac
     8341 aaaatggcgc atattgtaac cctaaatacc ccatccagag aagattggtt aacgcaactt
     8401 gccgatgttg tgaccgatcc tggtgaactt ctgcgtcttt tgaatataga cgcggacgaa
     8461 aaactgttgg ccggacgcag cgccaaaaag ctgtttgccc tgcgtgtgcc ccgctcattt
     8521 atcgatcgca tggagaaagg caatccggac gatcctattt tgcgtcaggt acttacctcg
     8581 caagatgagt ttgtcgtcgc gtccggattc tccaccgacc cactggaaga acagcacagc
     8641 gtagtgcctg gtttgttgca taaataccac aaccgggcgc ttttgctggt caaaggcggc
     8701 tgcgcggtaa attgccgcta ttgcttccgt cgccacttcc cctatgccga aaatcagggc
     8761 aacaagcgta actggcagac tgcacttgag tatgttgctg cgcatccgga actggacgag
     8821 atgattttct ccggcggcga tccgctgatg gcgaaagatc acgagctgga ctggttgctc
     8881 acacaactgg aagccatccc gcatatcaaa cgtctgcgga ttcacagccg tctgccgatt
     8941 gtgatcccgg cacgtatcac cgaggcgctg gttgaacgct ttgcccgttc tacgctgcaa
     9001 atcttgctgg tgaatcacat caatcatgcc aatgaggtag atgaaacgtt ccgtcaggcg
     9061 atggctaaat tgcgccgtgt cggtgtcacc ctgcttaacc agagcgttct gttacgtggt
     9121 gtgaacgata acgcacaaac gttggcaaac ctgagtaatg cgttgttcga tgccggcgtg
     9181 atgccgtatt acctgcatgt gctagataaa gtacagggcg cggcgcattt tatggtgagt
     9241 gatgacgaag cacggcagat tatgcgtgag ttgctgacat tggtgtcggg atatctggtg
     9301 ccgaaactgg cgcgagaaat tggcggcgaa cccagcaaaa cgccgctgga tctccagcta
     9361 cgccagcagt aactgattaa acagggaagt taaacattag cctgatgcgc tacgcttatc
     9421 aggcctacat tgtgcattga aattgaatgg atttgcaatg aactgtaggc cggataaggc
     9481 gtttacgccg catccggcaa gagcaacaaa cgcaagtaca gcaacctgtc attaaactgg
     9541 gaaatttaat ctttgcccgt tgcataacct ttaattattc atatacattg ctgttgttac
     9601 tctttatata gccaacacca ataattaacc tttcttttct gtttatatag agtgagtacg
     9661 gctatttatg ttattttaac aactattact accaagccca tttatctggc ttataaatta
     9721 aaattaatta tttaattgag aaatggttag ggagaaccta catggccata agcatcaagg
     9781 gtgttaatac cggtgttatt cgcaagagta ataatttcat tgcactggcg ctgaaaatta
     9841 aagaacctcg c
//
LOCUS       DAEEKQ010000141         9848 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.143, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000141 DAEEKQ010000000
VERSION     DAEEKQ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9848
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.143"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..144)
                     /locus_tag="KLK57_003281"
     CDS             complement(<1..144)
                     /locus_tag="KLK57_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893575.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVREITKMMNGKLIYSWLKWQCG
                     EFFS"
     gene            222..1316
                     /gene="ddlA"
                     /locus_tag="KLK57_003282"
     CDS             222..1316
                     /gene="ddlA"
                     /locus_tag="KLK57_003282"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HBE0893576.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALKRHAADNALKATM"
     gene            complement(1340..1552)
                     /locus_tag="KLK57_003283"
     CDS             complement(1340..1552)
                     /locus_tag="KLK57_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HBE0893577.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1812..2120
                     /locus_tag="KLK57_003284"
     CDS             1812..2120
                     /locus_tag="KLK57_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HBE0893578.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2179..3273)
                     /gene="yaiW"
                     /locus_tag="KLK57_003285"
     CDS             complement(2179..3273)
                     /gene="yaiW"
                     /locus_tag="KLK57_003285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HBE0893579.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLAITFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3286..4506)
                     /gene="sbmA"
                     /locus_tag="KLK57_003286"
     CDS             complement(3286..4506)
                     /gene="sbmA"
                     /locus_tag="KLK57_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HBE0893580.1"
                     /translation="MFKSFFPKPGAFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGWLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4858..6015
                     /gene="ampH"
                     /locus_tag="KLK57_003287"
     CDS             4858..6015
                     /gene="ampH"
                     /locus_tag="KLK57_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HBE0893581.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6016..6639)
                     /gene="iprA"
                     /locus_tag="KLK57_003288"
     CDS             complement(6016..6639)
                     /gene="iprA"
                     /locus_tag="KLK57_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HBE0893582.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6727..9615)
                     /locus_tag="KLK57_003289"
     CDS             complement(6727..9615)
                     /locus_tag="KLK57_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0893583.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATYNTFGYHDDAATIYN
                     WGDSTKIDHDYLTYGGYVYDHAADGYFDTTFSGDTVNGVISTYYLNHDYGTDTGNTLN
                     ISNSIVHGMVTSYQVGYGDYVWTNGSDYTGHDWVDGDIFTLNIANSTIDDDFDAFYFT
                     DTYLDADGKTSKTDYDRLVTAGLGTAVTLDVESNINISNNSHVAGITLVQHDLGNATY
                     NTEGHQWDNNIVVNDSTVTSGSWTEDEQSDRGHFGNSVEPSDYGNGASPADDVALAFI
                     DDDTSDYRMVNNVTFNNSQLLGDVVFDSTWNANFDATGHLIDNSTTAYTHGGWATDDQ
                     NVDHLNLTLNNTKWVGSANIDYDVVVADEAFYDVAPNSLNPYASYSEDGWNRVDNANA
                     FQSGVFDVVLNNGSDWETTKTSLIDTLAINSGSQVNVSADSSLTSDTITLNGGSSMEV
                     NGEVNTDHLTIDTFSTVNFGEDTASAWAYAPLYANTITVTNGGVLDVNTNMNDISSVF
                     ATDTLELTSGNVKDNNGNVYAGVFDIHSNDYILNADLVNDRTNDTSKANYGYGVIAMN
                     SDGHLTVNGNNDINNGDEVDNSSADNVVAATGNYKVRIDNSTGAGAIADYAGKQLIYI
                     DDTKTNATFSAANKADLGAYTYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNL
                     EQDTVGTRLTNSRHGLADNGGAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKVDGN
                     NAKWIVGAAAGFAKGDMNDRSGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDL
                     SATMSNGTYVDGSTNSDAWGFGLKAGYDFKLGDAGYVTPYGSVSGLFQSGDDYQLSND
                     MKVDGQSYDSMRYELGVDAGYTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNG
                     TEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2543 a         2426 c         2364 g         2515 t
ORIGIN      
        1 agaaaaaaac tcaccgcatt gccatttaag ccaggaataa attagcttcc cgttcatcat
       61 ttttgtgatc tcgcgcacat ctttgagata acgcactttc ttcaccagta cgttgcacgc
      121 tttgtcagca gaagattgca acaatctttc agaaggccgc gaatcccccg ttttgtcgtg
      181 gtaatgtatg cactttgtgt tagacgggat aggtttttaa gatggaaaaa ctgcgggtag
      241 gaatcgtttt tggtggtaaa tcagcggaac atgaagtgtc tctgcaatcg gcaaaaaaca
      301 ttgtcgatgc cattgataaa agtcgcttcg acgttgtgct gctgggcatt gataaacaag
      361 ggcaatggca cgtcagcgat gccagcaatt atctgctaaa tgcagacgat cctgcccata
      421 ttgcgttgcg cccttcggcg accagccttg cgcaggtgcc aggtaaacat gagcatcagc
      481 ttatcgacgc gcaaaacggt cagccgttgc cgacggtgga tgtcattttc ccgattgtcc
      541 acggtacgct gggcgaagat ggttccttgc agggaatgct gcgggtcgcc aatttaccgt
      601 ttgtaggttc tgatgttctg gcttcagcag cctgtatgga taaagatgtc accaaacgtc
      661 tgctacgcga tgccgggctg aacattgcgc catttattac cctgacgcgc gctaatcgtc
      721 acaacatcag ttttgccgaa gtggagtcta aactggggtt accactgttt gtaaaaccgg
      781 ctaatcaggg ctcttctgtt ggtgtcagca aagtaaccag tgaagaacag tacgcaattg
      841 ccgtcgatct ggcgttcgag ttcgatcata aagtgatcgt tgagcaaggg atcaaaggtc
      901 gtgagatcga atgcgcagtt ctgggcaacg acaatccgca agccagcacc tgtggcgaga
      961 tcgtactcac cagcgatttc tatgcctacg acaccaagta cattgacgaa gatggcgcga
     1021 aagtggtagt tccggcagcc attgcaccag agatcaacga taagatccgg gcgattgccg
     1081 ttcaggctta tcagacgttg ggatgcgcag gcatggcgcg tgtagacgtg tttttaaccc
     1141 cagagaacga agtggtgatc aacgagatca acacactgcc tggcttcacc aacatcagta
     1201 tgtatccgaa gctgtggcaa gccagcggtc tgggttacac cgatctgatc acacgtttga
     1261 ttgagctggc gctgaagcgt cacgctgcgg ataacgcatt gaaagccaca atgtaatatt
     1321 ttaacgcccg ataatattct tattgggcgt tttcagtttc ttcatcgcga cgacgaataa
     1381 tcaaccccgc cagccagaaa ctaatcaccc acgtcaccag accgacggca taggtctgcc
     1441 aaccttttgc ttcaaatccc agtaacccca ccacgccatt aagaatgaat ataaggccaa
     1501 tggcgaacgc atagtagtgc cagtcacggc ggatttttac aggcagattc atggcagctc
     1561 cgttaacgaa aaacaacatc cttgcatatt ctgtaacaac cgtctgtggt gtagacaaca
     1621 ttttcgtaat tgttaataaa tttaacttaa ggttgccatt ttgtaactca caacgaagat
     1681 tcacgcttcg ggaatattcc caacatcaat ttaccatgat tctgatattg acaatctaaa
     1741 tgacctgtca gacttgccat aagccgctgg tatcacacca gaagagaagc agatgacttc
     1801 cggaggtttt tatggctgat ttcaccctgt caaaatcgct gtttagcgga aaatatcgca
     1861 atgcctcttc aacgcctggc aacattgcct atgcgttgtt tgtgctgttt tgcttttggg
     1921 ctggggcgca attgctgaac ctgttagtgc atgcgcccgg cgtctatgag cgtttaatgc
     1981 aggtccagga aacaggtcgc ccacgggtgg aaattggttt aggtgtcggc accattttcg
     2041 ggctgatccc gtttttagta ggctgcctca tttttgcagt ggtggcgcta tggctgcact
     2101 ggcgacatcg ccgccagtaa gatttacgtt gccagatacg gcctaaaacg ccttatctgg
     2161 catgacattc ctgtttgatc actgtttcat acaacgcgcc cgccgttcgt ctacgcgctt
     2221 cgcaaaccag gccgtagtca ggttgcgggt gattttcggg ctttccagtt gaatgccagg
     2281 caacatttcg cggggtagtg atttacccgt tttcgcttcg gcaagttgat aaactttctt
     2341 gtacagcgcc gtctcctcaa aagagaagct gtcacctttc tctaactgac ggcgaatttc
     2401 gctgtcgctc atccccagtt ttcccgccag tttgcgcgtt gccagttcgg ttttcccggg
     2461 ttctttactg tcgtaacgaa ttaaatcacc gtccagtgcc agcttcacgc cgctggcctt
     2521 actgactgcg ttctgaaacg cggcattacg gctggcgtac cagccagcgt taaaatcagc
     2581 aaagcggtat atcggtgcgc tatagctggc gggataattc agtaaatggt aagtaccaaa
     2641 ccacaacccg ccgcggcgac tgaagacttc ctgacgaact gtaccgtcca ttttccacgg
     2701 ataccctttg gtatgctgtt cggcaaaagc aatgctgacc tgcatcggcc caccggtgcg
     2761 caccggatta agcgaaccaa acagcgtctg ccccattggt accatgctga tcagatcgtc
     2821 aaaaatcgcg ctcaattgct tttctgtacg caccgaatct aaccgttcgc tataactttt
     2881 gccgtttggc gatttgattt ttagcgcggt atgaaccaga aaagcaggaa tgtgcatccg
     2941 ttcagcacga cggtcaatct cttgccaggc gatcttgctt aagcccggaa cggccggatc
     3001 tgcctgataa tttgactcct gctgcgccac cgccagcacc gagcagacgt tttccagcgt
     3061 cggtgccagc ccctgacttt caaaggtgat ggcgagatct ttcgcccagg catcgcggtc
     3121 tttcacgctc gcaggcatct tctgccgcac gacgctcgct acatctatcg ctttttcgcc
     3181 ctttttcaac ggttgcggag cctgactact acaccccgct aaaaccagaa ccgccaacaa
     3241 tgacagcgaa gatagatgat tgacgcgcga cattacttct cctttttagc tcaaggtatg
     3301 ggttacttcc tgaattttgt caccatccag ctcatgttca aagctgcgca gacgtttgta
     3361 gatagacatc aactcaacca gtgtggtcca tgagttaatc aggtactgga aagcaccgcg
     3421 aacctgacca aaaacgttgg taatctgcgt catcaggccg agcgtaatcg taccggcaac
     3481 aattgacgga aacagcaaga acaaaccgaa aacgttatcg acctgcaaat agagaatgcg
     3541 ggcgatgttg aaatacatat agtgaaaata gaggcgaaaa tagtttttcc gtacggcgct
     3601 aaacagctcg cgtaccgtag gcggcgtcgc gcgcgtggca tcgtcttcac cataaaccag
     3661 ctctttacgg taggcggcct ctactcgctg gtttttaaac tccagccccg gcagtttgat
     3721 ccctaccact gccagccatc cggtccccat cagcgaccag acgatagcgg caatcaccag
     3781 accatacgga atgtgcccga taatcggcag ctccggcaca tgcgcggaga gcgttaccag
     3841 caccggcagg aaggcgatca acgtcatgat ggcgttgata aaactgacgc ccatattctc
     3901 cagcgttgaa gcaaaacgca tggtgtcttc ctgcacacgc tgtgcggcac cttcgatatg
     3961 acgcagttgt tgccagttcg ccatgtaata ttcgttcatc gccgtacgcc agcggaacac
     4021 atagtgactg acaaagaagt tgttcagtac actgatcacc accgcgatca gcgcaatccc
     4081 cagaaagacg cccacttcgc ggtaaaattg ttcgatagtg actttatgcg gcgaacttag
     4141 cgcggtttga atcagatcat agaacggtgc ataccaggcg ttgacggcga ccccgacttc
     4201 caccaaaaac caggtgacga agatgatcag tgcagtaccg agtattgacc agtattgcca
     4261 acggtgcggg ctgtagataa accagaaaaa tgcaaaaagt cctacgcaaa caatgtagta
     4321 agcgtaaaaa atcaggaaat ccaacgacca gaaacgcgcg gcgctaatcg ggatctgccc
     4381 ggaagcgccg gtgatacgcg ccacccagtc tcccccaccg gcttgccaga agataacggc
     4441 aatcaatgcc caaacaaagg ccgagagaaa aaacgctccc ggctttggga aaaaagactt
     4501 aaacatatgc actcctgcta acttcttatc gttttaacaa ctgctgtgta ttaaccgcat
     4561 gaccactaca aagcgtcagc caaatgaaac cgcccattgt gacccatagc aaggcgctta
     4621 gttcgccagc ctgaccataa gaattgtttc gacggatttc acaaaccaaa gattgtttac
     4681 ataagcggat taacacgcgc aggatcacaa atgcaggcat tggtgagcag gaaaaagatc
     4741 gtgtttacgc gccgttacgt ttttaacact attattgcac taattcgccc tttgcaatcc
     4801 atcaatgagt agtataaata cgctcagtta ccttcattca atctatggac accaccgttg
     4861 aaacgtagtc tgcttttttc tgccgtgctg tgtgcggcgt cattgacctc cgtccatgcg
     4921 gcgcagccga tcactgaacc ggagtttgcc tctgatattg tcgatcgtta tgccgatcat
     4981 attttttacg gcagcggtgc aacggggatg gcgctggttg ttatcgacgg taaccagcgc
     5041 gtctttcgta gttatggcga aacgcgacct ggtaataacg ttcgcccgca gctggattcg
     5101 gtcgtgcgta ttgcttccct caccaaacta atgaccagtg aaatgctggt gaaattgctc
     5161 gaccagggga ccgtaaagtt aaacgatccg ctaagtaaat atgctccgcc aggcgcacgc
     5221 gtgccgacct acaatgggac gcccattacc ctagtgaatc tggcaaccca taccagcgcc
     5281 ctgccccgtg aacagcccgg tggcgcggca catcgtccgg tgtttgtctg gccaacgcgc
     5341 gagcaacgct ggaaatacct ttctacggcg aagctgaaag ccgcgccagg cagtcaggcg
     5401 gcgtactcta accttgcgtt cgacctgctg gcagatgcac tggcgaatgc gtccggcaag
     5461 ccttataccc agttgtttga agagcagatt acccgtccat tggggatgaa agacaccacc
     5521 tacaccccct caccggatca gtgccgccgt ttaatggttg ctgagcgcgg tgccagtccg
     5581 tgcaataaca cgctggcggc aattggcagc ggcggcgtct attccacgcc tggcgatatg
     5641 atgcgctgga tgcagcagta tttgtcatct gatttttatc agcgcagtaa ccaggcagat
     5701 cgcatgcaga cgttgattta tcagcgcgcg cagtttacga aagtgattgg catggatgtc
     5761 cccggcaaag cggatgcgct tggtttaggc tgggtatata tggccccgaa agagggtcgt
     5821 ccggggatta ttcagaagac aggcggtggt ggcggtttca ttacctatat ggcgatgatc
     5881 ccgcagaaga atattggcgc gtttgtggtg gtcactcgtt cgccgttgac gcgctttaaa
     5941 aatatgagcg atggcatcaa tgatctggtg accgagctaa gcgggaataa accgttggtt
     6001 atccccgcat cctgattaat actctgaagg caaacggttg atagcgacca gtttgccttt
     6061 attcatttcg atatagccgc ctttacgcaa agcagcgaga acttctgcga cgacagaacg
     6121 cgatatgcgt gtacgttgat ggatataatt catcacacca atacgtgatc gcaattcttc
     6181 attccagtca atcattgata ataatgttgc gcggatttgt tcgtaggaat tatgcccaat
     6241 gagctgcacg tcgcgtaatt ccagaattct attttgccaa gctaaccagt aaaaagcgtc
     6301 tcgccagagt tgattttgtt caataagcgt aatggtttgt ttggctggta gatgatatcc
     6361 cgtacaattt ccttctgata ttaatttgta tggtatatcg tttttcatta aaccatcagc
     6421 cagccccata atataagggg cctgggtaat accgataagt acgttttctt ctctacgcag
     6481 agagataact ccctctaaaa taacgaaagt atcttcgtta ttgacatcac tggaaaatat
     6541 aacttgcttt tcattattaa actcgaagcg cgtaccgtat ctggacaaac atttatcgag
     6601 cttaccaaat tcctgaagag gtttaactac agataacatt tgcgcgtcct ttgcagtaat
     6661 gcccgtcaaa tccttgacgg gcattattta gattaaatta ccagtatttc ttcggagtga
     6721 agaatattac caggtatatt taacacccac gttcgcggac cagtcttgat ctacgtcacc
     6781 accaccgagg tagttagcat cggtataggc gctgaagttc ttggtgaagc tgaactgagt
     6841 acccagacca acacgtaccg cagacccttc agtaccgtta tcgatggaat caccgttcac
     6901 atcgttatcg ttgttagagt cgtcgtagac gtaagccagt ttgaagtacg gagtcagagc
     6961 ctgatcttcg ctgtaggtga aggtataacc tgcatctaca cccagttcat aacgcatgct
     7021 gtcgtaagac tgaccgtcaa ctttcatgtc gttgctcagc tggtagtcat caccagactg
     7081 gaacagacca gaaacgctgc cgtaaggagt cacgtaacca gcatcaccca gtttgaagtc
     7141 gtaaccggct ttcaagccga agccccaagc gtcggagttg gtgctaccgt caacgtaagt
     7201 accgttgctc atggttgcag acaggtcgtt gttgaagtga gagtagctca agctaccatc
     7261 aacaaagacg ttgttcgcga agtgagcaga agagtagatg taggcagtct ggctgtcttg
     7321 atctacctga ccagaacggt cattcatgtc acctttagcg aagcctgcag ccgcaccgac
     7381 gatccactta gcgttgttac cgtcaacttt ggtatcaaca ccgaccatga tgccgttaac
     7441 atcctgatca tagttgatgg tgccgttgtc gccgttgaag ttaccaccga agtagcttac
     7501 ccatgcgccg ccgttatcag ccaggccatg acgagagttg gtcagacgag taccaacggt
     7561 gtcttgttcc aggttccaga tattggtgtt cgcagacggg atgctcagcg ccatgttagc
     7621 gtagtcggtc agctccatct gttgcagaac aacggtgtta ccgcgctgtt cagcctgata
     7681 ggtgtatgca cccaggtcag ctttgttagc agcagagaag gtcgcgttgg ttttggtatc
     7741 atcgatatag atgagctgct tacctgcgta atcagcaatc gcaccagcgc cagtggagtt
     7801 gtcgatacga actttgtagt taccggttgc agctacaacg ttgtctgcag agctgttgtc
     7861 aacttcgtcg ccattgttaa tgtcgttgtt accattaacg gtcaagtgac catcagagtt
     7921 catcgcaata acaccataac cgtagttagc tttgctggtg tcgttggtac gatcgttgac
     7981 gaggtctgca ttcaggatgt aatcattact atgaatgtca aacacacctg cgtaaacatt
     8041 accgttgtta tctttaacgt tgccactggt cagttccaga gtatcagttg caaatacgct
     8101 ggagatgtca ttcatgttgg tgtttacatc cagcacacca ccgttagtaa cggtaatggt
     8161 attggcatac agcggtgcat atgcccaagc tgaagcagta tcttcaccga agttaacggt
     8221 tgagaaggta tcaatggtca ggtgatcggt gttaacttca ccgttaactt ccattgaaga
     8281 accaccgttc agagtgatgg tgtcagaagt cagagaagaa tctgcactca cgttaacctg
     8341 agaaccgctg ttgatcgcca gggtatcgat cagggaagtt ttcgtggttt cccaatcaga
     8401 accgttgttc agaacaacat cgaatacgcc gctctggaat gcgttagcgt tatcaacacg
     8461 attccagcca tcttcagagt aagaagcgta cgggttcagg ctgtttggtg caacgtcgta
     8521 gaaggcttcg tcagcaacaa caacgtcata atcaatattc gctgaaccaa cccatttggt
     8581 gttgttcaga gtcaggttca ggtgatcgac gttctgatcg tcagttgccc aaccgccatg
     8641 agtgtaagca gtggtagagt tgtcaatcag gtgacccgtc gcgtcaaagt tagcgttcca
     8701 ggtactgtca aatacaacgt caccaagcaa ctgagagttg ttgaacgtaa cgttgttgac
     8761 catacgataa tcagaagtgt catcatcgat gaaggctaac gcaacatcat cagcaggaga
     8821 tgcgccattg ccgtagtcac ttggttcaac agaattgccg aaatgaccac gatctgattg
     8881 ttcgtcttca gtccatgaac cagaagttac agtagagtca ttaacaacga tgttgttgtc
     8941 ccactgatga ccttcggtat tgtaggtcgc attaccgagg tcgtgttgaa ccaaagtaat
     9001 acctgcaaca tgggagttgt tgctgatgtt gatgttactt tcaacatcca gagtcacagc
     9061 ggtaccaaga ccagcggtta ccaaacgatc ataatctgtc ttagatgttt taccgtctgc
     9121 atccagataa gtatcggtga agtagaaggc gtcaaaatca tcgtcaatag tagaattagc
     9181 gatgttcaga gtgaagatgt cgccatcaac ccagtcgtgg ccagtataat cgctaccgtt
     9241 ggtccagacg tagtcgccat aaccaacctg gtaagatgta accataccat gaacaattga
     9301 gttggagatg ttcagtgtat taccggtatc tgtgccataa tcgtggttca gataataagt
     9361 agaaattaca ccatttacag tgtcgccgct gaaagtggta tcaaagtaac catcagctgc
     9421 gtggtcatat acataaccac cgtaagtcag ataatcgtga tcaattttag tgctgtcacc
     9481 ccaattgtag atggtggctg cgtcatcgtg atacccgaag gtgttataag tagctgcatt
     9541 agcctgagag gtgatagcca gagtgcaagc caatgctaat tgtgatacta caagtttctt
     9601 tttccaggag tgcatttgtc atccctcctc agggacgtaa taagttgatc catcaattat
     9661 caatgcatag aaaacatatt atgtttcgcg ctaacgatta tgctgcgtta cagacgaatg
     9721 gttcaattat tctttcccct aagtccgaat gtggacatat ataatttatg gagaaaaaca
     9781 cgcatcaaat tgataaaagg acgatatttc aacatgttgc agataatgtt atttttgata
     9841 catacgcg
//
LOCUS       DAEEKQ010000142         9822 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.144, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000142 DAEEKQ010000000
VERSION     DAEEKQ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9822
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.144"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(39..1034)
                     /gene="gapA"
                     /locus_tag="KLK57_003290"
     CDS             complement(39..1034)
                     /gene="gapA"
                     /locus_tag="KLK57_003290"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HBE0893584.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            1376..1789
                     /gene="msrB"
                     /locus_tag="KLK57_003291"
     CDS             1376..1789
                     /gene="msrB"
                     /locus_tag="KLK57_003291"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HBE0893585.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSQTKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            1831..2103
                     /locus_tag="KLK57_003292"
     CDS             1831..2103
                     /locus_tag="KLK57_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HBE0893586.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKANCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(2264..2905)
                     /gene="pncA"
                     /locus_tag="KLK57_003293"
     CDS             complement(2264..2905)
                     /gene="pncA"
                     /locus_tag="KLK57_003293"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HBE0893587.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(2916..3932)
                     /gene="ansA"
                     /locus_tag="KLK57_003294"
     CDS             complement(2916..3932)
                     /gene="ansA"
                     /locus_tag="KLK57_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HBE0893588.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(4099..5955)
                     /gene="sppA"
                     /locus_tag="KLK57_003295"
     CDS             complement(4099..5955)
                     /gene="sppA"
                     /locus_tag="KLK57_003295"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HBE0893589.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6116..6667
                     /locus_tag="KLK57_003296"
     CDS             6116..6667
                     /locus_tag="KLK57_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HBE0893590.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            6784..7827
                     /gene="selD"
                     /locus_tag="KLK57_003297"
     CDS             6784..7827
                     /gene="selD"
                     /locus_tag="KLK57_003297"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HBE0893591.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDATEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKATAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            7832..9793
                     /gene="topB"
                     /locus_tag="KLK57_003298"
     CDS             7832..9793
                     /gene="topB"
                     /locus_tag="KLK57_003298"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HBE0893592.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIIPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERALDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLLQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDVVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
BASE COUNT         2435 a         2453 c         2616 g         2318 t
ORIGIN      
        1 ccgaagtcgc tctttttaga tcacagtttc atctcaactt atttggagat gtgagcgatc
       61 aggtccagaa ctttgttgga gtaaccggtt tcgttgtcgt accaggatac cagtttcacg
      121 aagttgtcgt tcagagcgat accagcttta gcatcgaaca cggaagtgca aacttcgccg
      181 ttgaaatcgg tagatactac gtcatcttcg gtgtagccca gaacgccttt catttcgcct
      241 tcagcagcgg ctttaacggc agctttgatc tgctcgtaag ttgcagcttt ttccagacga
      301 acggtcaggt caactacgga tacgttcggg gtcggaacgc ggaacgccat accagtcagt
      361 ttgccattca gttctggcag tactttacct acagctttag cagcaccggt agaggacggg
      421 atgatgttct gggaagcgcc gcggccgccg cgccagtctt tgtgagacgg gccatcaacg
      481 gttttctgag tagcggtagt agcgtgaacg gtggtcatca gaccttcgat gatgccgaag
      541 ttatcgttga taactttagc cagcggagcc aggcagttgg tggtgcagga agcgttggaa
      601 acgatgtcct ggccagcata tttgtcgaag ttagcgcctt taacgaacat cggagtgttg
      661 tctttagacg gaccagtcat aaccactttc ttcgcaccag cggtgatgtg tttacgagca
      721 gtttcgtcag tcaggaacag accagttgct tcagcgacaa cgtcaacacc aacttcgtcc
      781 catttcaggt tagccggatc acgttcagcg gtaacacgga tttttttacc gttaacgatc
      841 agatgaccgt ctttcacttc aacggtaccg tcgaaacggc cgtgagtgga gtcatatttc
      901 agcatgtatg ccatgtaatc agcgtctaac aggtcgttga ttgcaacgat ctcgatgtca
      961 gaacgtttct gagcagcacg gaaaacaatg cgaccgatac ggccaaaacc gttgatacct
     1021 actttgatag tcatatattc caccagctat ttgttagtga ataaaaggtt gcctgtaaaa
     1081 ttacaaaaac cttacgcagc gtcaagcgga atcgtgtcaa tcattgcgac aaatcaatcc
     1141 tgtgcctaag cattacgcga ctgactcgcc tcactcttcc tttgggcttg aaaccacatg
     1201 gggtcggcgc cccgaatttt aaagggcaat tacgataaaa atgtgatttt catcacgatt
     1261 taggtgcagc catttcgtcg agatcaagtt taggacaaaa ttttgccagt tggtgttaat
     1321 gttttgttag aatcagtcac gtaatgtgag cacgattaaa gtgagatgtg agcaaatggc
     1381 taataaacct tcggcagaag aactgaaaaa aaatttgtcc gagatgcagt tttacgtgac
     1441 gcagaatcat gggacagagc cgccatttac gggtcgttta ctgcataaca agcgtgacgg
     1501 cgtatatcac tgtttgatct gcgatgcccc gctgtttcat tcccaaacca agtatgattc
     1561 cggctgtggt tggcccagtt tctacgaacc ggtgagtgaa gaatccattc gttatatcaa
     1621 agacttgtca catggaatgc agcgcataga aattcgttgc ggtaactgtg atgcccatct
     1681 ggggcatgtc ttccctgacg ggccgcagcc aacgggcgaa cgttattgtg ttaactctgc
     1741 ttctttacgc ttcactgatg gcgaaaacgg cgaagaaatc aacggttgaa aaaacgattc
     1801 agcaacttat tccacaggag cggaaaaatt atgaatcttg atgacattat caacagcatg
     1861 acgcctgagg tataccagcg tttgtcgacc gccgttgaac tggggaaatg gcccgatggc
     1921 gttgcgttga ccgaggaaca aaaagcgaac tgcctgcaac tggtgatgct gtggcaagct
     1981 cgccataata ccgaagcaca gcatatgaca attgacacaa atggtcagat ggtcatgaag
     2041 agcaaacagc agttaaaaga agattttggt atctcagcaa agcctattgc tatgtttaag
     2101 tgaatgagtt atctagtatt taagcctgtt attaagggag ggcaaatata cttgttagga
     2161 cgtcgtgttt tagctgcaag taggttaaat gcaacactga caagtattat ctggcctaca
     2221 atgtcattga ggaaatgtag gccagagatg aattcaacta atattacccc tgtgtctctt
     2281 cccagtctgc cagcgtatat agcgttgccc cagctgctga catctccata aacgcgtgcg
     2341 cactgtcctg gggctggata ttcacgccac gacaaccatc ggtaatcaca tttaccttat
     2401 aacctaactg taacgcgtcc agcacggtaa acttcacgca atagtcagta gccaggccca
     2461 taacgatcaa ttcatcgatt tcatgatcgc gtaaccagtc atcgagcgag gttttctgcc
     2521 gacggccgtt atcaaaaaag gcactgtaac tgtcaactaa aggattttcg cctttatgga
     2581 acaccgctgc gatcgctttt tggttcagta acggatgtaa ttgtgcgcct tcactgttct
     2641 gcacacagtg atctggccag aaggtttgtg gcaaaccgtc gagttggcct ggcgtataag
     2701 gctctacacc gtgctgactg gcaaaactgc cgtgattcgc cgggtgccag tcctgactgg
     2761 cgataaccgc ttcaccgcgc gactggcacc agtcaatcag gcggttagcg acatccaccg
     2821 tactgtcacc ttccggcacg gcgagcgcgc caccagcaca gaaatcattt tgtaaatcga
     2881 ccagtaacag ggcgcgaggg ggcattacag tctccttaat catccggcgt cagttcgccg
     2941 cgcaggtttt ggctcatggc cttgcgaatg gtttcagtat ccagttcctg gctcagcagg
     3001 taatgcagtt tggttagcgt ggcttctaca gtcatatctg caccgccaat aacgccggca
     3061 tgggcgaggg cgttaccggt ggcataacca cccatattca ctttaccgga catacattgt
     3121 gtcaggttga ccaccacaat accgcgatcg ctggcttctt gtaattcctg caggaaggct
     3181 ttgttttgtg gcgcattacc cacgccatag gagcgcagaa tcaatgcttt caccggttgg
     3241 cgcagaaaat tgcgcacgac gtcagcagaa atccctggat aaatcgtcac tacgccaatt
     3301 ggttgtggcg tgattggatg aacgattaat tccccttcac cgtgcggggc gggtggcgta
     3361 ttcaaacgac gaatatggat accagcttcc agtaacggag gaaggtttgg agaggcaaac
     3421 gcatcaaaac catcggcatg ggctttggta gtgcggttgc cgcgatacaa tcggttattg
     3481 aaaaagagcg ttacttcgtt gatcggataa ttcgccgcaa cgtacaacgc attcagcaga
     3541 ttaatttgtc cgtcagagcg taactcagcc agcgggattt gtgaccctgt cacaatgacc
     3601 ggtttaccga gattctcgag catgaacgac agcgcagagg cagtatacgc catcgtgtcg
     3661 gtgccgtgca ggatgacaaa accatcatag tcgtcatagt gcgctttaat atcttcagca
     3721 atatgctgcc agtcttctgg cgtcatatca gaagaatcca tcagcggcgt atattcatga
     3781 atggtgaaat ctggcatctc cgggcgatgg aattccggca tcagcgccag ttggcgttgt
     3841 agatgacctg acaccggaat ataaccctgc tcggaacgct gcatcccgat ggtcccgccc
     3901 gtgtaggcga cgtaaattga tttcttttgc atgatattga tatgaatgat atcgaaagag
     3961 caaaagtata tacgtgaggc aggaggagcg cacctgatac tatgcggata aggaaatatg
     4021 gggcgagaca gcgggaagag gattcgtcga cgtaaaaaat aatgtggccg acgatctgtc
     4081 ggccactcag tacaagactt aacgcacgtt ggcgcaggtc aggcaaaacg cataacggtt
     4141 ttgtgggtcg ttaaacgcgg ccagcttgtc gctttcactt ttaacggtag aggctaccga
     4201 ggcaagcggt gcaggtaaca tggcctggaa cgcatctggc aacattgccc ggacagaacc
     4261 agacatgttg tccatcactt tgtcgaagaa ggtcggttca tcaacgtagt attccagatg
     4321 ccactgtttc acttttgcca gctctgctgc tttggcgacc gcatcatcga aatccccgag
     4381 actatcgacc agcccgttag cttttgcatc ctgaccggtc cagacgtggc cctgggcgat
     4441 tttatcaatc tgctccggcg tcgaatgacg cgcatcagca accagcgtga taaagcgttt
     4501 atagccattc tcaatgctta attgcatcat ctgctgcgct tccggcggca gtgccctggt
     4561 gatagaaaca tccgccagcg gtgaagttga gacaccatca gtatgaacac caatcgaatc
     4621 cagactattt tctacggtgg tgatcacgcc gaagataccg atagaaccgg tcagggtgct
     4681 ggggttagcc acaatgtaat tagctggcgt ggaaatccag taaccaccag atgccgccat
     4741 gccgcccatc gatacaacca caggcttacc cgctgcccgg gctgctgcca gttcagcgcg
     4801 aatcacttca gacgcggtaa cgctgccgcc tgggctatta acacgcagga caatcgcttt
     4861 cactttcggg tcaaggcgag cgtcgcggat ttgtgccgca gtggtatcac cgccaacatt
     4921 cccctgagtt tcctcgccat ccataattgc gccattagcg aagacgacac cgatgctgtc
     4981 accggtatct gccggcgttt tcaatgcgta atcgtaataa ctgatggcgc gataattttt
     5041 atcagtctta ctccagccga actctttggt tagagctttt tcgatttcgg cactcgatgc
     5101 cagtgcatcg accagcttgt tttccagtgc atatttcgcg gtatcgccac cggttttggt
     5161 taaaccctca agcaaccctt gcgcgccagg gaatacctgc tgagcaggga tctgccggtt
     5221 agcggcaaca gtattcagat agttttgcca cagctcacca atccagcggc tgtcagcttc
     5281 gcgggctgcc ggtgacatgt catcacgaat aaacggttca acggcagatt tatacgtacc
     5341 cacgcggaac acatgggtgg aaactttcag cttatccagc aacgatttgt agtacagacc
     5401 gttggtagca aaaccgtgca aatccaccac gccttgcggt gacagccaaa ttttattggc
     5461 gaaactggcg agataatatt gcccctggct gtagttctcg ccaacggcat aaaccggttt
     5521 cccgctgtca cgaaactctt tcagggcttt gccgatgtac tgcatagacg gctggtcgcc
     5581 gcctgcgaag tttttcagat ccatcacgat accggtgata ttgcggtcgt ccttcgcctg
     5641 gcgaatggtg ttgacgatat caaacagtga gttttcctgc agacgatcgg aactggcacc
     5701 aagcagctgg cggcttaatt tactaaaccg ctgagaactg tcgggtttat cgacgatcac
     5761 accagaaatg tccagcagca gtgcgccacg actggccgtt tctttcgaat caccaccact
     5821 gacctgcatc cagatcccca caccaaccag tacgaggaaa ataaagaaca ggttaagtac
     5881 catttcacgg acgaaattca gcagacgcca cgtccattta aaaaatccgg caataaatcg
     5941 ccaaagggtt cgcatgtatt ctcccaactt aaggtcacac ctgtcacagg cgcaatagat
     6001 gtggttatcg taatgacaca accggcactt gtcagcagga attgcggact atgctgtaac
     6061 aaaatccact ctcgtgttaa ttttgtgaat aaatatcacg acaggagtta atcaaatgga
     6121 tgcactcgaa ctattgatca atcgccgtag cgcctcccgc ctggctgaac ccgcgccaac
     6181 gggtgaacaa ctgcaaaaca tcctgcgtgc gggtatgcgt gcgccggacc ataagtccat
     6241 gcaaccgtgg catttttttg tgattgaagg ggaagggcgc gagcgtttca gcgccgtact
     6301 ggaacagggg gcgattgctg ccggtagtga tgacaaagct atcgacaaag cccgtaatgc
     6361 gccgttccgc gcaccgctca tcatcacggt ggtggcgaaa tgcgaagaaa atcataaagt
     6421 cccgcgatgg gaacaggaaa tgtctgccgg atgcgcggtc atggcgatgc aaatggcagc
     6481 cgttgcccag ggatttggcg gcatctggcg cagtggcgca ttaactgaaa gtccggtagt
     6541 gcgtgaagca ttcggttgcc gtgagcagga taaaattgtc ggttttctct acctcggcac
     6601 gccgcagttg aaagcatcta cgtcgattaa cgtcccggac ccgacgccgt ttgtaactta
     6661 tttctgataa ttgtcgcgaa actgtctgga ttgtgagcaa aggcgcagga attcagacac
     6721 tctcacttat cacttcacgg aatgagggtt accatacccg gattgcattg acaggagatg
     6781 tccatgagcg agaactcgat tcgtttgacc caatacagcc acggagctgg ttgcggctgt
     6841 aaaatttccc caaaagtgtt ggaaaccatc ctgcacagtg agcaggcgaa gtttgttgat
     6901 ccgaatttgc ttgtgggtaa tgagacccgc gacgatgcgg cggtgtacga tctgggcaat
     6961 ggcaccagcg ttatcagtac caccgacttc tttatgccga tcgttgataa tcctttcgat
     7021 tttggccgca ttgcggcgac taacgccatc agcgatatct tcgcgatggg gggcaaaccg
     7081 attatggcga ttgcgatcct cggctggccg attaacaaac tttccccgga aattgcccgc
     7141 gaagtaaccg aaggtggacg ctatgcatgc cgtcaggcgg gtattgcgct ggctggcggt
     7201 cactccatcg atgcgacgga gccgattttt ggtctggcgg taacggggat cgtaccgacc
     7261 gagcgggtga agaaaaacag taccgcacaa gccggatgca aactgttcct gacgaaaccg
     7321 ctggggatcg gcgttcttac cacggctgag aaaaaatcac tgctgaaacc agaacatcag
     7381 ggactagcga cggaagtgat gtgccggatg aacatcgcag gcgcgtcctt tgccaacatc
     7441 gaaggcgtaa aagcgatgac cgacgttacg ggttttggtc tgctgggcca tttgagcgaa
     7501 atgtgtcagg gcgcaggtgt gcaggcgcgc gttgactatg acgcgatccc aaaattgccg
     7561 ggggttgaag agtacattaa gttgggcgca gtgcctggcg gcaccgaacg taactttgcc
     7621 agctacggtc atctgatggg tgaaatgccg cgtgaagtgc gcgatttgct gtgcgatccg
     7681 caaacttctg gcggtttgct gctggcggtc atgccggaag cagaaaatga ggtcaaagct
     7741 acagccgccg agtttggcat tgaactgacg gcaattggcg aactggtgcc agcgcgcggc
     7801 ggtcgtgcca tggttgagat tcgttaattc aatgcggttg tttattgccg aaaaaccgag
     7861 tctggcgcgc gccattgctg atgtcctgcc caaaccgcac cggaaaggcg atggctttat
     7921 cgagtgcggt aatggtcagg tggtgacctg gtgtatcggt cacctgcttg agcaggcgca
     7981 gccagacgcc tacgacagcc gctatgcgcg ctggaatctt gcggatttgc cgattatccc
     8041 ggaaaagtgg caattacagc cccgaccctc cgtgaccaaa caacttaacg tcatcaaacg
     8101 cttcctgcat gaagccagcg aaatcgttca cgccggggac ccggatcgtg aagggcaact
     8161 gctggtggat gaagtgctgg actatctgca actggcaccg gaaaagcgcc agcaggtgca
     8221 gcgttgcttg ataaacgacc tgaacccgca ggcggttgag cgggcgctcg accgtcttcg
     8281 gtccaacagt gagtttgtgc cattgtgcgt ttctgcgctg gcgcgagcgc gtgccgactg
     8341 gctgtacggc atcaatatga cccgtgcgta taccattctc ggtcgcaatg ccggttatca
     8401 gggcgtactt tccgtgggac gcgtgcagac acccgtgctc gggctggtgg tgcgccgcga
     8461 tgaagagatt gaaaacttcg tggcgaaaga cttctttgaa gtcaaagcac atatcgtgac
     8521 acctgccgat gagcggttta ccgctatctg gcaaccgagc gaagcgtgtg aaccgtacca
     8581 ggatgaagaa gggcgcttgt tacatcgtcc actggcggag catgtggtta accgcattag
     8641 tggtcaacca gcaattgtca ccagctataa cgataaacgg gaatcagaat ccgcgccgct
     8701 gcctttttcg ctttcggcgt tgcagattga agcggcaaaa cgctttggtc tgagcgcgca
     8761 gaacgtgctt gatatctgcc agaagctgta cgaaacgcac aagctaatca cttatccgcg
     8821 ttccgattgt cgctatttgc cagaagaaca ttttgccgga cgccacgcgg tgatgaatgc
     8881 catcagcgtt catgcaccag atctgttgct gcagccagtg gtagatccag atatacgcaa
     8941 ccgctgttgg gatgacaaaa aggtcgatgc acaccacgcc atcatcccga ccgcacggag
     9001 ttcagccatc aacctgacgg agaacgaagc gaaggtctat aacctgattg cccgtcagta
     9061 cttgatgcag ttctgcccgg atgtggtgtt ccgcaagtgt gttatcgaac tggacattgc
     9121 caaaggcaaa tttgtcgcta aagcgcgttt tcttgctgaa gcaggctggc gcacgctgtt
     9181 aggcagcaaa gagcgcgatg aagaaaacga cggtacgcca ttgcctgtgg tggcgaaagg
     9241 cgatgagttg ctgtgtgaaa aaggtgaagt ggtagagcgg caaacccagc cgccgcgcca
     9301 ttttaccgat gcaacactgc tttcggcgat gaccgggatc gcgcgctttg tgcaggacaa
     9361 agatctgaaa aagatccttc gtgcgaccga tggtctgggg acagaagcaa cccgtgccgg
     9421 gattattgaa ctgctgttca agcgtggttt tctgaccaaa aaagggcgct atatccactc
     9481 taccgacgcc ggaaaagcgt tattccattc gctgccagaa atggcgacgc gaccggacat
     9541 gaccgcgcac tgggaatcgg tgctgacgca aatcagcgaa aagcagtgtc gctatcagga
     9601 ctttatgcag ccgctggtgg ggacgctata tcagcttatt gatcaagcca aacgtacgcc
     9661 ggtgcggcag tttcgcggca ttgtggctcc gggcagtggt ggcagtgctg ataagaaaaa
     9721 ggctgcaccg cgtaaacgta gtgcgaaaaa aagtccgcca gcagatgaag tcggaagcgg
     9781 ggcgatagcg taagcgagtg aatctttcgt gctattcgag tc
//
LOCUS       DAEEKQ010000143         9798 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.145, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000143 DAEEKQ010000000
VERSION     DAEEKQ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.145"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..191
                     /gene="bdm"
                     /locus_tag="KLK57_003299"
     CDS             <1..191
                     /gene="bdm"
                     /locus_tag="KLK57_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HBE0893593.1"
                     /translation="STAEPALVNAIEQGLRAEQGVVTEDDILMELTKWVEASDNDILS
                     DIYQQTINYVVSGQHPTL"
     gene            293..430
                     /gene="sra"
                     /locus_tag="KLK57_003300"
     CDS             293..430
                     /gene="sra"
                     /locus_tag="KLK57_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HBE0893594.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            587..2284
                     /gene="maeA"
                     /locus_tag="KLK57_003301"
     CDS             587..2284
                     /gene="maeA"
                     /locus_tag="KLK57_003301"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBE0893595.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2418..3420
                     /gene="adhP"
                     /locus_tag="KLK57_003302"
                     /pseudo
     CDS             2418..3420
                     /gene="adhP"
                     /locus_tag="KLK57_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415995.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
     gene            3566..3850
                     /locus_tag="KLK57_003303"
     CDS             3566..3850
                     /locus_tag="KLK57_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HBE0893596.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="KLK57_003304"
     CDS             complement(4078..4731)
                     /gene="fdnI"
                     /locus_tag="KLK57_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HBE0893597.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="KLK57_003305"
     CDS             complement(4724..5608)
                     /gene="fdnH"
                     /locus_tag="KLK57_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HBE0893598.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEERVAKLKARGYEHAGVYNPKG
                     VGGTHVMYVLHHADQPELYYGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="KLK57_003306"
     CDS             complement(5621..8668)
                     /gene="fdnG"
                     /locus_tag="KLK57_003306"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8081..8083),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBE0893599.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8900..9781
                     /gene="yddG"
                     /locus_tag="KLK57_003307"
     CDS             8900..9781
                     /gene="yddG"
                     /locus_tag="KLK57_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HBE0893600.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2358 a         2576 c         2496 g         2368 t
ORIGIN      
        1 attcaacagc agaacccgct ctggttaatg ccattgagca aggccttcgg gcagagcaag
       61 gtgttgtcac tgaagatgac attctcatgg agctgaccaa atgggtggaa gcttctgata
      121 acgacatcct cagtgatatc taccagcaaa cgatcaatta tgtggtcagt ggccagcacc
      181 ctacgcttta aggtgctatg cttgatcggc aacctaattt aggggtttag cacgtgtttc
      241 ttcgctacgg cgatgttgtc cttaaaacta gcaacaggat tgaggagtta aaatgaaatc
      301 gaaccgtcag gcacgtcata ttcttggact ggaccataaa atttctaacc agcgcaaaat
      361 agttaccgaa ggtgacaaat ccagcgtggt aaataaccca accggcagaa aacgccccgc
      421 tgaaaagtaa ttcataacca tcagtcctca atgacgatta aacaccattg cctgcgcaat
      481 ggtgtttttg tttttatctg ctttatactt ggggccgacg ccctggcggt aaagcaaaga
      541 cgataaaagc gtgccaggga tggatattca aaaaagagtg agtgacatgg aaccaaaaac
      601 aaaaaaacag cgttcgcttt atatccctta cgctggccct gtactgctgg aatttccgtt
      661 gttgaataaa ggcagtgcct tcagcatgga agaacgccgt aacttcaacc tgctggggtt
      721 actgccggaa gtggtcgaaa ccatcgaaga acaagcggaa cgagcatgga tccagtatca
      781 gggattcaaa accgaaatcg acaaacacat ctacctgcgt aacatccagg acaccaacga
      841 aaccctcttc taccgtctgg taaacaatca tcttgatgag atgatgcctg ttatttatac
      901 cccaaccgtc ggcgcagcct gtgagcgttt ttctgagatc taccgccgtt cacgcggcgt
      961 gtttatctct taccagaacc gccacaatat ggacgatatt ctgcaaaacg taccgaacca
     1021 taatatcaaa gtgattgtgg tgactgacgg tgaacgtatt ctggggcttg gtgaccaggg
     1081 catcggcggg atgggcattc cgatcggtaa actgtcgctc tataccgcct gtggcggcat
     1141 cagcccggcg tatacccttc cggtggtgct ggatgtcgga acgaacaacc aacaactgct
     1201 taacgatccg ctgtatatgg gctggcgtaa tccgcgtatc actgacgacg aatactatga
     1261 attcgttgat gaatttatcc aggctgtgaa acaacgctgg ccagacgtgc tgttgcagtt
     1321 tgaagacttt gctcaaaaaa atgcgatgcc gttacttaac cgctatcgca atgaaatttg
     1381 ttcttttaac gatgacattc agggcactgc ggcggtaaca gtcggcacac tgatcgcagc
     1441 aagccgagcg gcaggtggtc agttaagcga gaaaaaaatc gtcttccttg gcgcaggttc
     1501 agcgggatgc ggcattgccg aaatgatcat cgcccagacc cagcgcgaag gattaagcga
     1561 ggaagcggcg cggcagaaag tctttatggt cgatcgcttt ggcctgctga cggacaagat
     1621 gccgaacctg ctgcctttcc agaccaaact ggtgcagaaa cgcgaaaacc tcagtgactg
     1681 ggataccgac agcgatgtgc tgtcactgct ggatgtggtg cgcaatgtaa aaccagatat
     1741 tctgattggc gtctcaggac agaccgggct gtttacggaa gagatcatcc gtgagatgca
     1801 taaacactgt ccgcgtccga tcgtgatgcc gctgtctaac ccgacgtcac gcgtggaagc
     1861 cacaccgcag gacattatcg cctggaccga aggtaacgcg ctggtcgcca cgggcagccc
     1921 gtttaatcca gtggtatgga aagataaaat ctaccctatc gcccagtgta acaacgcctt
     1981 tattttcccg ggcatcggcc tgggggttat tgcttccggc gcgtcacgta tcaccgatga
     2041 gatgctgatg tcggcaagtg aaacgcttgc acagtattcg ccgctggtgc tgaacggcga
     2101 aggtctggta ctaccggaac taaaagatat tcagaaagtc tcccgtgcaa ttgcgtttgc
     2161 ggttggcaaa atggcgcagc agcaaggcgt ggcggtgaaa acctctgccg aagctttgca
     2221 acaagccatt gacgataatt tctggcaagc cgaataccgc gactaccgcc gtacctccat
     2281 ctaagcctgc gcccggtagt gaaggctacc gggcaatttt cctctccctt tttcagatct
     2341 catccatact gggtagtggc gaataaatct catttgcctc acctgctatg cagaacatca
     2401 tccgaaaagg aggaactatg aaggctgcag ttgttacgaa ggatcatcat gttgacgtta
     2461 cggataaaac actgcgctca ctgaaacatg gcgaagccct gctgaaaatg gagtgttgtg
     2521 gtgtatgtca taccgatctt catgttaaga atggcgattt tggtgacaaa accgacgtaa
     2581 ttctgggcca tgaaggcatc ggtgtggtgg cagaagtggg tccaggtgtc acctcattaa
     2641 aaccaggcga tcgtgccagc gtggcgtggt tctacgaagg atgcggtcat tgcgaatact
     2701 gtaacagtgg taacgaaacg ctctgccgtt cagttaaaaa tgccggatac agcgttgatg
     2761 gcggaagagt gcatcgtggt cgccgattac gcggtaaaag tgccagatgg tctggactcg
     2821 gcggcggcca gcagcattac ctgtgcgggg gtcaccacct acaaagccgt taagctgtca
     2881 aaaattcgtc ccgggcagtg gattgctatc tacggtcttg gcggtctggg taacctcgcc
     2941 ctgcaatacg caaagaatgt ctttaacgcc aaagtgatcg ccattgatgt caatgatgag
     3001 cagttaaaac tggcaaccga aatgggtgca gatttagcga ttaactcacg caccgaagac
     3061 gccgccaaaa ttgtgcagga gaaaaccggt ggcgctcact ctgcggtggt aacagcagta
     3121 gctaaagctg cgtttaactc ggcagttgat gctgtccgtg caggcggtcg tgttgtggct
     3181 gtcggtctgc cgccggagtc tatgagcctg gatatcccac gtcttgtgct ggatggcatt
     3241 gaagtggtcg gttcgctggt cggcacgcgc caggatctaa ccgaagcctt ccagtttgcc
     3301 gccgaaggca aagtggtgcc gaaagtcgcc ctgcgtccgt tagcggacat caacaccatc
     3361 tttaccgaga tggaagaagg caaaatccgt ggccgtatgg tgattgattt ccgtcactaa
     3421 gaggtctttg ctgcgactcc catgttcggg tcgcagcatc gcacactctc caacatgaaa
     3481 tggctaaatg gattattgtt gcgtaggaag gaacaataat gatagtgttc ccagagaagg
     3541 aacgctactg agagtaaaga cactaatgaa aatggccaat catccccgcc cgggggacat
     3601 tattcaggaa tcactggacg aacttaatgt cagcctgcgc gagtttgcca gagcaatgga
     3661 aattgcgccc tcaacggcaa gccgattgct gaccggaaaa gcagctttga cgccagaaat
     3721 ggcaattaaa ctttccgtgg tgatcggcag ttcgccgcaa atgtggctga atctgcaaaa
     3781 cgcctggagt ctggcagagg cagaaaaaac ggtggatgtg tcgcgattac gccgtctggt
     3841 tacgcaataa aaaatggcac cttataaagc gccatttgaa actgatgaca aacgcaacat
     3901 tgcctgatgc gctacgctta tcaggcctac acgttcactt caatctattg tatttgaagg
     3961 gttttcatgc cggataaggc attcacgacg catccgggat gaacaaagcc cacgttgtct
     4021 acaatatgaa aatggcgcct tttacagcgc cattttcagc ttaaagttca aaagagatta
     4081 tatcccttct tcactctctt ttttcgcttc tgccttctcg atttcacgat accagcgcgg
     4141 atggtgtttc ttcgcccagc gacggctcac cttcccttcg atcatccctt taatcgaccc
     4201 tttcacccag aatgccatat acatatggat caggatggcg tggatcagga tgatacccgc
     4261 agccgcgtgg atcagcaggc tgtagcgaac aacctgcatc gggaagtact gcgcaaagta
     4321 cggacgccag ataatcaccc cggtcaccag cagcacgaaa atcatgctca tgatcgacca
     4381 gaacatcatc ttttgcccgg cgttgtactt accaacatcc gccactttgt gctcattgcc
     4441 ttttaatact tcgacaatgt tcaacagcca cggaatatct ttcttatccg ggatgttgtg
     4501 atgcacaaaa cgcacaaaca taaacatcag tgcgacgaaa atcgcaatgc cgaagaacgg
     4561 gtgcaaaatg cgtcccatct gcggcgtacc gaaggtttgc gtcagccatt gcagcgtcgg
     4621 gaagaagaac gaaatcccgg acagcgccac caggaagaag caaatcacca cggtccagtg
     4681 acaggcgcga tcaataaatt tggtgcgcac aatcattttc gacttactca tgatgatcct
     4741 cctcgtcatc gtccacttcc ttattcgggc caataccgat gtagtggaaa atcaacccgg
     4801 caaaagtggc aataaagcca gccgctgcca gcggtttcaa cgcgcctttc cacaggctta
     4861 ccgaggtgtc gatcttcgga tctttcggca gaccgtaata cagctccggc tgatcggcgt
     4921 gatgcagcac gtacataacg tgcgtaccac cgaccccttt cgggttgtag acgccagcat
     4981 gttcgtaacc acgcgctttc agtttcgcca cgcgctcttc cgccagctcc agcatctcct
     5041 tcttggtgcc gaagtggata gccccggtcg gacaggtttt cacacaagcc ggttcctggc
     5101 cgacgctgac gcgatcgacg cagagcgtgc atttatatac ccggttatcc tctttgttga
     5161 ggcgcggaat attaaacgga cacccggcaa tgcagtaacc acagccgatg cagttttccg
     5221 actggaaatc gacaatcccg ttagcgtact gaatgattgc accagcagac gggcacgcct
     5281 tcaggcagcc gggatcttca cagtgcatac agccgtcttt acggatcagc cactccagct
     5341 tgccgttctg ttcggtttcg ctaaagcgca tcaccgtcca ggacttggcg ctcagatcgg
     5401 cggggttatc gtaaaccccg acgcagtgcc ccacttcatc acggatgtcg ttccactccg
     5461 aacacgccac ctgacaggct ttacagccga tacaggtgga aacgtcgata agttttgcga
     5521 cttctgcttt gtaatcacgc acctgagaag gcggcgtgat ggagttagtt gcggaccttt
     5581 tgataatatc ctgcgtttcc atagccatct gttcgccccc ttacgccttc tcgatgttga
     5641 ctaagaacgc tttatattcc ggcgtttgcg agtttgcatc accgacattc ggcgtcagag
     5701 tgttagcgat ataaccttta cgcgcgacac cctcaaagcc ccagtggatt ggaataccca
     5761 ccgtttcaac ctgctgaccg tttacattca gcggtttcag acggcgcgtt accaccgcca
     5821 ccgcgcggat aaagccacgc ttgcttgaga cagtgacacg atcgccatta ttaatgcctt
     5881 tcgccgccgc cagcgtttcg ctgatttcca caaactgttc cggctgagca attgcgttga
     5941 gcaatgcgtg cttggtccag gtgtggaagt gctcggtcag acgataggtc gtacccacat
     6001 acgggaactg ctctttttta cccatccgca gcgcgtcttg ttcatacaga cgaacaaccg
     6061 ggttagacac cacgttcgga tgcagcgggt tagtgcccag cggcgtttca atcggctcgt
     6121 agtgttccgg gaacggacct tccgccattt tgttgatggc aaacaggcgt cccatccctt
     6181 ccggctgcat gataaacggc ccggttggcg tacccggtgc ggcattgccg aagtccggaa
     6241 tatcgttacc cgtccacttg ctgccgttcc actggatcag catccgtttc ggatcccacg
     6301 gtttaccatt gatatccgcc gaagcacggt tgtagagcac gcgacggttg agcggccacg
     6361 cccaggccca tcccagcgta ttccccagac cggatgggtc ggagttatcg cggttagcca
     6421 tctggttgcc ctgctctgtc cagctaccgg tgtagatcca gcaagaagat gcggttgtac
     6481 cgtcatcacg cagatgcgca aagctactca gcaactgacc tttcttcgca atcagcacgc
     6541 cattagcgtc atagagatct tccagcgcat agccgttatt ctctttcgcc acttcgtcag
     6601 attgcggttc gtgcggctgc ttgtagttcc agctcatctt catcagcggt tctacgcctt
     6661 taccaccttc ggcctggtac agctcgcgca gatgatggta gatacccgcc agaatttcgc
     6721 cgtcgttacg cgcttcgccc ggcgcgtcct gacctttcca gtgccactgc aaccagcgac
     6781 cggagttagc gatagaacca tcttcttcgg caaagcaggt cgaaggcaga cggaatactt
     6841 cagtctgaat agacgccgga tcgacatcgt tcgactcgcc gtggttctgc cagaaggtag
     6901 aggtttcagt caccagcgga tcgataacca ccatgtactt cagcttgctc aggcagctca
     6961 ccactttgtt tttgtccggg aaggacgcaa ccgggttaaa gccctggcag aagtaaccgg
     7021 tgactttgcc ttcgtccatc atgttgaaat acttgatgac gtcgtaggtc tggtcccact
     7081 tcggcagcca gtcatagccc cagttgttct ctttctgcgc ggcatcgcca tagaaagatt
     7141 tcatcaggct aacgaagaac ttcggatagt tgctccagta gttcacctga tcagccagcg
     7201 tcgctttcgg cgtgttcgct tccagatacg actgcaaatc aacctgtttt tctgacggaa
     7261 gcgtcagata acctggcagg ctggtagaga gcagacctaa gtcagtcagc ccctgaatgt
     7321 tggagtgacc acgcaatgcg ttcacgccgc caccggccat acccatgtta ccgagcagca
     7381 actggatcat cgccatagta cggatgttct gcgcacccac ggtgtgctgc gtccagccca
     7441 gcgcgtacag gaaggtggtt gtgcgatccg gtgcgctggt ggaggccagc acttcacaca
     7501 ctttcaggaa gtcggctttt ggcgtaccgc agatgttttc aacgacgtcc ggcgtgtagc
     7561 gggaaacgtg cgctttcagc aggttccaca cacagcgcgg atgagtcagt gtttcatcgc
     7621 gttttgcata gccgttttca tcgaactgat agttccagga cgacttatca tactggcgtt
     7681 tttcagcgtc gtagccgctg aacaggccgt cttcgaaagc aaaatcatca cgcaccagca
     7741 ggctggcgtt ggtgtaatgt ttaacgtatt cggcgttgat tttgttgttt tcgatcaggt
     7801 agcgcaaaac gccagacagg aacgtaatgt ccgtaccgga acgaataggt gcgtaaatat
     7861 ccgccacaga agcggtacgc gtaaaacgag gatcgacaac gatcaaggtt gcatcgttgt
     7921 tgtttttcgc ttccatcgcc cagcggaaac cgacgggatg cgcttcagca gcgttaccgc
     7981 ccattaccat tacgacgtta gcgtttttga tatccaccca gtggttggtc atcgcaccgc
     8041 gaccaaatgt tggagcaaga cttgctaccg ttggtccgtg tcagacgcgc gcctggttgt
     8101 ctaccgccag catcccgagg gagcgggcaa atttctgggt cagcatcccg gtttcgttgc
     8161 tggcaccgga ggcacacagc ataccggtag aaagccaacg gtttaccgtt acgccctgct
     8221 cgttcttttc aataaagtta gcgtcacggt cagctttcat cagcttcgca atacgggaga
     8281 atgcttcttc ccagctaatg cgctgccatt tgtcagaacc tggcgcacga tattccgggt
     8341 agcgcagacg gttttcgctg ttgacgtaat ccagcaaacc ggcccctttt gggcacagcg
     8401 caccacggct taccggatga tccgggtcac cttcaatgtg ataaatcgct tctctggcgt
     8461 ttttcgcgcc atcacccagg ctatacatca atagcccgca acctacggaa cagtatgtgc
     8521 aggtgttacg gatctcttta gcgcgtaata atttgtagtt tcgcgcctga gccagtgctt
     8581 gcttcggggc aaagcccaat gccgctaccg ttgttccagc cataccgccc gcgcagattt
     8641 taaaaaattg tctgcgactg acgtccattg ctttcctctt ttttcagggg tattactgcg
     8701 cgaggaaact aacagtaaaa ttctgaatct ttttgcgcga aatcaaaaag aacacgtgta
     8761 tgccacaaaa taatccgcct gcgcaaatta acctacctca atagcggtag aaaaacgcac
     8821 cactgcctga caggccagtt aaaaaaatgc tataaaattc agcttaattt ttaacggcaa
     8881 gagagacaaa acagcgagca tgacacgaca aaaagcaacg ctcatagggc taatagcgat
     8941 cgtcctgtgg agcacgatgg taggattgat tcgcggtgtc agcgaggggc tcggtccggt
     9001 cggcggcgca gctgctatct attcattaag cgggctgctg ttaatcttca cggttggatt
     9061 tccgcgtatt cggcagattc cgaaaggcta tttactcgcc gggagtctgt tattcgtcag
     9121 ctatgaaatc tgtctggcgc tttccttagg gtatgcggcg acccgtcatc aggcgattga
     9181 agtgggtatg gtgaactatc tgtggcccag cctgacaatt ctctttgcca ttctgtttaa
     9241 tggtcagaaa accaactggc tgattgtacc aggattatta ttagccctgg tcggcgtctg
     9301 ctgggtgtta ggcggtgaga atgggttaca ttatgatgaa atcatcaata atatcaccac
     9361 cagcccattg agttatttcc tggcgtttat tggtgcgttt atctgggcag cctattgcac
     9421 agtaacgaat aaatacgcac gcggatttaa cggaattacc gtttttgtcc tgctaacggg
     9481 agcatgtctg tggatttact attttctcac gccacaacca gaaatggtat ttagcacgcc
     9541 cgtcatgatt aaactcatct ctgcggcatt taccttagga tttgcttatg ctgcatggaa
     9601 tgtcggtata ttgcatggca atgtcaccat aatggcggta ggctcgtatt ttacgcctgt
     9661 actttcctca gcgcttgcag ccgtgctgct cagcgccccg ctgtcgttct cgttctggca
     9721 aggcgcgcta atggtctgcg gcggttccct gctctgctgg ctggcgacac gtcgtggtta
     9781 aaataattgc cgggtcta
//
LOCUS       DAEEKQ010000144         9644 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.146, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000144 DAEEKQ010000000
VERSION     DAEEKQ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.146"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..667)
                     /locus_tag="KLK57_003308"
     CDS             complement(<1..667)
                     /locus_tag="KLK57_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HBE0893601.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIQ
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLNQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDD"
     gene            complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="KLK57_003309"
     CDS             complement(939..4841)
                     /gene="hrpA"
                     /locus_tag="KLK57_003309"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415931.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HBE0893602.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKIRKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQVMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDKLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            5042..5647
                     /gene="azoR"
                     /locus_tag="KLK57_003310"
     CDS             5042..5647
                     /gene="azoR"
                     /locus_tag="KLK57_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HBE0893603.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLVTGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(5698..7014)
                     /locus_tag="KLK57_003311"
     CDS             complement(5698..7014)
                     /locus_tag="KLK57_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HBE0893604.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDSNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAVR
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGACACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGYCKTVDEAISFIRARRSHIVLKEDHKAMLKLWENR"
     gene            complement(7004..8761)
                     /locus_tag="KLK57_003312"
     CDS             complement(7004..8761)
                     /locus_tag="KLK57_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HBE0893605.1"
                     /translation="MENSRISGEHFFTTSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHIVDELAMPDTAFYAWDARGHGKTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALTLWHR
                     LRGLFFINSYVKRRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFIRRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGT
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHVKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKLWVMRVRTQGEMDLLVHDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(8777..9640)
                     /locus_tag="KLK57_003313"
                     /pseudo
     CDS             complement(8777..9640)
                     /locus_tag="KLK57_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
BASE COUNT         2448 a         2627 c         2273 g         2296 t
ORIGIN      
        1 tatcatcgcg taaacgcggc agctcgccag tgagtagtga gagataacgc tcaaccggcc
       61 ataatccttg taactgatta ataaagatta cactgtctgc gttatttcct aactgaggaa
      121 cgaatccggg ataacttaac cagcgtggtg catcgggatt gtccccagtc atacggcgga
      181 acgtcgccat cgtgcgccgc tgcatggtgg ggtcctgaac aacgatagcc gtatgaactc
      241 gttctacggc ctgattcaat agcgcgatgc taaagcgtgc gttttcaccg cagtttgttg
      301 actggtcttc aatccagatt ttttcatgcg gaatgtgcca gaactgatga gcgatatccg
      361 cctggatggt cgcttctgct ctgccagtgg tgcggatagt gttgtagtgc ggatgctgtg
      421 cgatggcgct atacaaaaaa gttgtcgagt gaccgatacc accactaatc agtaaaggaa
      481 tttgctgatc gcgagcaatc ttacatgccg cgtcgatagt cggcataacc gcattgcctg
      541 caaggatcac gcaatcggcc tgatacggca cctcaccgga gaaatcatcc tgcgccagcc
      601 actgtccgat aacatttatg gcatctatag ttgccggcga aagcgtcgga aacggggtaa
      661 tgttcatggt tcctccttat cgttccctta agaatactgc gtgagataat agtgagaagc
      721 ggagcactct gaaaggagga agtcgtattt ccttaaccgc cactccgcaa gcctgacata
      781 tcttttaagg aaatgctgta gataaacatt attcatcttg cagcagagaa ttgtattttt
      841 ctgaattcct gaatttttac ccttaccgtt ttagaattaa taaaaaaccc ggcgctaaat
      901 gcttaccggg ttttttcttt atcaggcaaa tagcagggtt aaccgctaat ctgctccatc
      961 gcctgcaaaa tacgcttatc tgaaatcgga taaggcgtac caagttgttg agcgaagtaa
     1021 ctaacacgca actcttctat catccaacgg atctctttca cgtcttcatc ctcacgacgt
     1081 gcaggcggca gtttgttgat ccattgctgc cacgcctgct ggacgttttc gactttcagc
     1141 atctgagcac ggtcgcgatg tggatcaacc gccagttttt ccagccgttt ttcaatcgcc
     1201 tgcaaatatc gcagcgtgtc gcccagccgt ttgaagccgt taccagtgac aaaaccgcga
     1261 tataccagcc cgcccatctg cgctttaatg tcagaaagcc ccagcgccat cgtcatatcc
     1321 acccgcccct tcagacgttt gttgatattg aacactgccg taaggatttg ctcgacctgc
     1381 ttcgcaatat ccaccaccgt gtcgttcagt tcggcacgca ctttttcatg cagcgcagca
     1441 aagccttctt ccgtccagac cgggccacca ttggcgtcga tcaatttatc cacaccgcag
     1501 gagatacagt cgtcgatcag ctccagcact ttgccatacg ggttaaagta cagccccagc
     1561 ttggctttgt ttggtaactt ctcatgtaaa tacttgatcg gcgagggaat attcagcagc
     1621 agtagacggc gaagaccgtt ccacattacc tgtttttgct ccagcggatt atcaaacagt
     1681 ttgatcgcca cactgtcgcg ttcatccact agcgctggcc acgccttcac tttgtagttg
     1741 ccacgcttct gttcgtagct ttccggcagc tgaccaaaac tccagatatg cagtccgctc
     1801 tgctcgatac cgtcatccgc caccgcagac agcgtttcct gcactttgcc tttcagcgca
     1861 tctttcagat cttgtagcga gcgcccttct tttagcttct tgtttttgtc atccaccacg
     1921 cggaaggtga ttttcaggtg atcgggcacc tgatcccagt gccagtcttc gcggtcaacg
     1981 gtaacgccgg tcatccgccg taactcgcgc tcaaggctgt cgagcaacgg taactccagc
     2041 ggtgtgacgc ggcctaaaaa cgcttcggcg tagtttggtg caggcacaaa attacggcgt
     2101 accggtttcg gcaacgattt aatcagagca atcaccaatt cgcggcgcag accggggatc
     2161 tgccattcaa acccgctttc ctcaacctgg ttaagtaacg gcagcggaat atgtacggtc
     2221 acaccgtccg cgtccgcgcc tggctcaaac tgatagctca aacgcagctt gagattgccc
     2281 tgatgccaga agttcgggta atccagcttt ctgatttttt ccgccccttc tttgatcaac
     2341 atgctctttt caaagttgag caaatcaggc gtttcgcggc tgactttttt ccaccagcta
     2401 tcgaagtgac gagcggagat tacatcgtgg ctgatgcgct ggtcgtagaa ctcaaacagc
     2461 gtttcgtcat caaccagaat atcgcggcga cgtgatttgt gttccagctc ttccacttcg
     2521 gcccgcagtt tcaggttttc acggaagaat gcgtgacgcg tctgccagtc accttccacc
     2581 agagcgtggc gaataaagag ttcacgacat aacgccgaat cgatctggct gtagttgacc
     2641 ttgcgcgcag caacaatcgg caaaccataa acagtgactt tttccgttgc catcaccgcg
     2701 ccctgcgccc gttcccagtg cggttcgctg taggtgcgtt taatcaaatg ctgagcaacg
     2761 ggttctaccc attccgggtc gatacgcgca gcaatgcgcc cccacaggcg gctggtttct
     2821 accagttccg ccaccattac ccatttcggc ggctttttga ataaaccaga accggggaag
     2881 atggagaaac gcgcgttacg tgcaccggta tattcctgtt tatcggcatc tttcatgccg
     2941 atatgggaaa gcaaaccggt cagtaaggcg atgtgaattt cacgatactc cgccggttcg
     3001 ctgttgaccg gaatgccaag ttctttcacc acctggcgca actgggtgta gatatcctgc
     3061 cattcgcgca cgcgcagata gttgaggtaa tcggtacgac acaggcgacg gaaggcgttg
     3121 gaagaaagcg ccttttgctg ctcgccaaga taattccaca gatttacaaa cgcgaggaag
     3181 tcggattctt tgtcgtggaa gcgacgatgt ttttcgtccg atgcctgctg tttgtccatc
     3241 ggacgttcgc gcggatcctg aatggagagc gcggacgtga taatcatcgc ctcacgcacg
     3301 cagccatgtt tttgcgcttc cagcaccata cgcgccagac gtgggtcgac aggcaactgc
     3361 gagagctggc gaccgagcgg cgtcagttta taggcgctgg cctgttcatc cgtggtgatc
     3421 gcgcccagtt cttcgagcag acgcacgcca tcctggatat tgcgtttatc cggtgcttcg
     3481 acaaacggga acgcagcgat atcgcccagc cccagcgcgg tcatctgcaa aataaccgag
     3541 gccaggttgg tacgcagaat ctccggatcg gtaaactccg ggcgcgagag gaaatcgtct
     3601 tcggaataga gacgaataca gatcccttcg gacacacgac cacagcggcc tttacgctga
     3661 ttggcagacg cctgcgaaat gggttcaatc ggcaaacgct gcactttggt gcgatagctg
     3721 tagcggctga tacgcgctgt accggggtcg atgacgtatt taatccccgg tacggtcagc
     3781 gaggtttccg cgacgttggt cgccagcaca atgcgccgtc cgctgtgcga ctggaacacg
     3841 cggttctgtt cgctgttcga aagccgcgca taaagcggca agatttcggt atggcgtaag
     3901 ttcagcttgt tcagcgcatc ggcggtatcg cgaatttccc gctcaccact cataaagatc
     3961 agaatgtcgc cagggctttc ctgactcagt tcgtctacgg cgtcaaaaat cgcctgcaac
     4021 tgatcgcgct cggtgtcatc ggcttcttca acaatcggtc gatagcgcac ttccaccgga
     4081 taggtccgac cggagacttc gataatcggc gcattattaa agtggcgcga aaagcgttcc
     4141 gggtcgatag tcgcggaagt gataatgatt tttaggtcag gacgccgcgg cagcaactct
     4201 ttcaaatagc cgagcagaaa atcgatattc aggctgcgtt cgtgcgcttc gtcaataatg
     4261 atggtgtcgt actgcatcag caggcggtct tgctggatct ccgccagcag gataccgtcg
     4321 gtcatcagct tgaccatcgt gttatcactt acgtgatcgc tgaaacgcac tttgtaaccg
     4381 atgcaaccgc ccggctccgt tttcagctct tccgcaatac ggttcgccac cgttcttgcc
     4441 gccaggcgac gcggctgggt atggccgatc agccctttaa tcccgcgccc cagctccata
     4501 cagattttcg gtaactgagt cgttttacca gaacccgttt ccccggcgac gatcaccacc
     4561 tggtgatcac gaatcgcttc gagaatgtcc tgttttttct gactaaccgg taaattgtca
     4621 ggataagtaa tttccggtcg tgccgcttca cgcagcagga ctttccctgc cgcctggtca
     4681 atctctttcg ccatctcctg gaaaatggcc tgttgtgcat caggattttt aaccttcttc
     4741 acgccgtgca gacggcgaga aaaacgcagt ctgtcacgca gcatcagcga atccagccgc
     4801 tgctgcaagg ccgtaaaggt caatttttgt tgttctgtca taacgttagt gggcagtagt
     4861 ctgcctgatt aattttcttt gaaatgatag atatagagta ccacatcacg gctttctatg
     4921 cgttgttcaa taaattcgaa cataggcttc gatttattgc gctatctctg tggcaggatt
     4981 gtgaataaag tgtcaacaag caacggggca tcgcccattc aaacatctat aaggaaacac
     5041 catgagcaag gtattagttc ttaaatccag catcctggca gggtactctc agtctaatca
     5101 gttgtccgat tattttgttg aacaatggcg cgaaaagcac tccgctgatg aaatcaccgt
     5161 tcgcgacctg gctgcaaatc cgattccggt actggatggc gaactggttg gtgcactgcg
     5221 tccgagcgat gcgccgctga ctccgcgtca gcaggaagct ctggcgcttt ccgatgagct
     5281 gattgccgag ctgaaagccc acgacgttat cgttattgcg gcaccgatgt ataacttcaa
     5341 catctcaact cagttgaaaa actattttga cctggttgca cgcgcaggcg ttactttccg
     5401 ctataccgag aacggtccgg aaggtctggt aacgggtaaa aaagccatcg ttattaccag
     5461 ccgcggtggg atccacaaag atggaccaac ggacctggtg acgccgtatc tgtccacgtt
     5521 cctcggcttt atcggcatta ctgatgtgaa atttgtcttc gccgaaggga tcgcatacgg
     5581 tccggaaatg gcagcgaaag cgcagtctga cgcgaaagca gccatcgaca gcattgttgc
     5641 tgcataaccg gacacgatct tgatgtggac tgtagtccag tccacatccc aatccactta
     5701 cctgttttcc cataatttca gcatcgcttt gtgatcttcc ttaagcacaa tatgcgagcg
     5761 tctggctcga ataaagctaa tcgcttcatc aacggtttta cagtatccgt aacataacag
     5821 ccatgccgcc actaccagcg cactgcgcga taatcccagt gcgcaatgga ccagaacgct
     5881 gccttgctct tcgcgtaatg tttccagcat cgccacggcc tgtcggagct ccccctcttc
     5941 cggaaccacc agatctagca tcggtacaca gaaatagagt cgatcttttg tcgctcgtcc
     6001 acgagggaat tcaaaggtga cgtccagaac cgcattctgt gccggaatat gtcgtggaaa
     6061 cgcccctaaa taaacaccag cagtcatttt gctcaccggc tccaggcgac gacaaaacca
     6121 acgcatagat agccacatcc cgatgcgcca gggcaatgtc agccagtaaa cggcgggcgg
     6181 gagtttcccc tgactatctt tccctgttgt tatcgcgcca agcccaccgt acccacggcc
     6241 aataatgagt agcgataata ctggccaaca taaccagact gaccaccata actgaaccat
     6301 catcattagc tccatcaaca caatgcacgc gcacgcgcct acgacatatg gcagtgctat
     6361 tttgattcga cgttgatcag gtttctgata attccaacga cggtcgacgg gcaccatcca
     6421 gtcaatcaac atacctaccg ccagccctgt gatgacatca ataaaatgat gctgccatgt
     6481 cgtcagcgtc gaaatggcga tgagtaaaaa ccatccgccg cagactttac gccacctcac
     6541 agccagatgc tgacgaaagt gacgccaaag tagccagcag agaataatat gcagcgaagg
     6601 agactggtta taaggcagat caaacagttc aagttgcgaa aatagccatc ccgtcacccc
     6661 actcacttca ggacggataa aactaaactt cagcggatag agcaaaaaac cgcagcaggc
     6721 cattaccgtt gccagaataa gccggtggac aagtcggcgc tgttcgaatg tcgtgctaca
     6781 aacgaacagc gaaaatccat ataaaagatc cagactccag taaggaacaa tagtccaggg
     6841 aagaaaaggg atcgccgttt cccaaccgaa tacctgactg ggaatatcat ggctattact
     6901 gtcctgaacc gcggtgaact gattaagaga tccataggtg aagaagaaaa acggggccag
     6961 caacaataac cagccagctc cttgtagcaa tacgttccgg cgttcagtta tcacgacgca
     7021 ccgccatcga aaccgtaaaa atgccccact catcaatccg ttgtgtgcat ttatcaaatc
     7081 cagcatcatg cacgagcaaa tccatctccc cttgcgtacg cacgcgcatt acccacagtt
     7141 taccatcttt atgactggtt aacaccccgg caatcagctc cagttgtggg tgccacggct
     7201 gcccggtgta gatgagaatg ccacccggtt cgatggcatt ggcaagacct gcgagtgagt
     7261 tttttacctg ctcattttcg ggaaacagct catacagtcc agagacaatc gccagcgtag
     7321 gccgcggagt taacgcgctg agttcctccg ggttaaacgc atcgccctgt tcaaaacgca
     7381 cccgcccaga cattccccgt tgggcaatca tctcttgccc ctgtgcaaca tttaactcac
     7441 tgtaatcacg taataaaata tcgcttacgg caggctcgtt tgccagtgca tccagtacat
     7501 agcgcccatg ccctgcggca atgtcaacca cgcggacggc taaacctttg acgtggagat
     7561 cggcaacggc ttgtttaatc agtatttgta aatgagtttt gcgctggcga ataccgcgcc
     7621 agccaacact gttcaggtaa attttgtcga ttaagcgccc gaatgcgtta ctgccttgcg
     7681 gttgattttg atagacataa tccagcgaac tgcctgaatc aaagccagtg ctcattccga
     7741 gatgcaggcc cgcagagtgc gtcccgaaca atttcatcgc tttacgcata aagcgatacg
     7801 ccaaatcaac cggcgataat ggcacgggtc cacctgaaag gaggcgccag cgatccgctg
     7861 atggtccagt gcggtcttca tgctgataat caaatttttg tgacttgtta gcatataaac
     7921 gacgaataaa gctttgcatt ttttcaaatg cctgcgccct gttctcttca cccaacgtgt
     7981 cgtgataaaa gcctggcagc agatgcagct ctttcagagg gctacgtaat ctctgataaa
     8041 aatcaatctg cggttggcga tgcaccacat agtcatcgcc tgatatcaga agttgcgtgg
     8101 ggagcgtaat cgccgccgca tctctaataa tacgttcaga cgttttatag agatcgagca
     8161 agatattgac ggcaatcgcc cgtgtgatca gcggatcatt attgaaactc gccacccgtt
     8221 gccgatcgtg ggtcaaatag cgtcttttca cataggaatt aataaaaaac agaccacgca
     8281 gacgatgcca tagcgtcagc gcaggacgtg ccagcggcac atacaattta accttaaagg
     8341 ccggagaagc cagcaccagc ccgcgaattg caggcgcata atcatgaacc catgtggcaa
     8401 ccagcactgc gccgacgctt tgcgcgatca caaccacctc ttccagtcca acctggctgt
     8461 cgctggcagc aaaacggaca aattcatcga catcctgcac tgaacgcgca agagatgggc
     8521 tataaccacg cggccccgaa gtttttccat gcccccgggc atcccatgca taaaaagcag
     8581 tatctggcat cgccagttca tcaacgatat gttgtagacg accagaatgt tcatggccac
     8641 gatgaaataa gacgatgacc tttttcgccc ctggctgtaa agcgggccag tgccgataaa
     8701 acaacgctgt attatcactg gtggtaaaaa aatgttcccc agagatgcgt gaattttcca
     8761 ttttctctat tttccttcag taacagcagt aacgaataaa ataaaaaaat accggagcgg
     8821 tgaaaattaa tgagtcaatc ctgtcaagga ttccgccatg tccaggtaat agtttcccgc
     8881 tatctttaac accaacatct cgtttgatgg ctgacatcac tacatcgccg caaaaaccac
     8941 taataccaat taacaaaccc gctaatattg cctgtaatgt atttagcggt gtcagtaacg
     9001 gtccaatgat catcgacgct atcatggtgg tgattacgcc gccgaccaga ccttccagcg
     9061 tttttcccgg gctgactttg gggaccactt ttcttctgcc gcaggatttc ccccataaat
     9121 actgtgcaat atcgtttgac gcggttaatg ccaaaagaaa taacaccagt aaggccccgc
     9181 cctggatatt catggatggc aacattaata actaggcggc atgactcaag gcgaaaacgg
     9241 tcattatcca gccccaaaaa atggcgctca cggtatgcag aaaaccggag gggtcaccca
     9301 caaaaactcg ccaggcggct aatatcagaa acccagccaa aggtataaat aacagaaaca
     9361 gctcaaagca attaaaaccg ataagcaaat agttaagagg aataacccaa tataaccacc
     9421 gcggaaaatg tacggatata agcatacaat attctttcaa tgccagaaaa cttatcagcg
     9481 caaaaaacgc caacgtcatc cagcgtggac ctgaaatcac cagtgaaaaa caaatgacga
     9541 tgccccacca ggtacgaata cgtaatgtaa cctcaccccc ttttctctcc ggtaaccgcc
     9601 atagcagaaa acggtttatc agcgttgcta aaattaacaa cgcg
//
LOCUS       DAEEKQ010000145         9578 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.147, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000145 DAEEKQ010000000
VERSION     DAEEKQ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.147"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..158)
                     /locus_tag="KLK57_003314"
     CDS             complement(<1..158)
                     /locus_tag="KLK57_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HBE0893606.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPA"
     gene            complement(158..1084)
                     /gene="glsB"
                     /locus_tag="KLK57_003315"
     CDS             complement(158..1084)
                     /gene="glsB"
                     /locus_tag="KLK57_003315"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HBE0893607.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(1148..2536)
                     /gene="sad"
                     /locus_tag="KLK57_003316"
     CDS             complement(1148..2536)
                     /gene="sad"
                     /locus_tag="KLK57_003316"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HBE0893608.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALELAAAGFRD
                     WRETNIDYRAEKMRDIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENTLGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            2637..3518
                     /locus_tag="KLK57_003317"
     CDS             2637..3518
                     /locus_tag="KLK57_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0893609.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRTNCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVSDSAKQSYQ"
     gene            3595..4710
                     /gene="yneK"
                     /locus_tag="KLK57_003318"
     CDS             3595..4710
                     /gene="yneK"
                     /locus_tag="KLK57_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HBE0893610.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFLNDFIEQF
                     PMFNPGVPIKIAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVLPEVCICFLFLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLELFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATSKTPDLPERH"
     gene            4860..6050
                     /gene="ydeA"
                     /locus_tag="KLK57_003319"
     CDS             4860..6050
                     /gene="ydeA"
                     /locus_tag="KLK57_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HBE0893611.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(6075..6740)
                     /gene="marC"
                     /locus_tag="KLK57_003320"
     CDS             complement(6075..6740)
                     /gene="marC"
                     /locus_tag="KLK57_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HBE0893612.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            6952..7386
                     /gene="marR"
                     /locus_tag="KLK57_003321"
     CDS             6952..7386
                     /gene="marR"
                     /locus_tag="KLK57_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HBE0893613.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            7406..7789
                     /gene="marA"
                     /locus_tag="KLK57_003322"
     CDS             7406..7789
                     /gene="marA"
                     /locus_tag="KLK57_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HBE0893614.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            7821..8039
                     /gene="marB"
                     /locus_tag="KLK57_003323"
     CDS             7821..8039
                     /gene="marB"
                     /locus_tag="KLK57_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HBE0893615.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(8096..9535)
                     /locus_tag="KLK57_003324"
     CDS             complement(8096..9535)
                     /locus_tag="KLK57_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HBE0893616.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
BASE COUNT         2467 a         2461 c         2163 g         2487 t
ORIGIN      
        1 gccggagcaa gacgtttagc gacaaaatcc gccgcatgtt gttcgatgac tggcgcgcct
       61 ttctccaggc aatactgacg ctctttcatg cccagacgaa agcgagagcg aaattttgaa
      121 cgggataaac gcgcgaagag tggatcgaga gactgcatta ataaaccgaa cgccctaact
      181 gtttcgtcaa ttgttcaagg acggcgatac ccgcaagcga gttacctgca tcatccagtt
      241 ccggactcca gactgcgatg gccatttcat gcggaacaat cgccacaata ccgccaccaa
      301 cgccagattt cgccggtagt cccacccgcc aggcaaactc ccccgcgttc tggtacatac
      361 cactggtcgc catcagcgcg ttaatttgcc gcgcctgcat tggcgtcact acaggttcat
      421 caatatgaat agctttcccc tgattagcca gaaagacaaa cgtccgagcc agctctacac
      481 agctcatttt cagagcgcag taatgaaagt agttttgcag aacggttgtc acgtcatgat
      541 ggaaattgcc aaacgacttc atcagccagg cgatagccgc atttcgcgcg gaatgttcaa
      601 attcggaacg cgctaccacc gtatcgtatg aaatatcaga cacaccgctt aagccgcgca
      661 cgacttccag catacgttgc cgtggtgcgc ttaatcgccc ttgcaacata tcgcagacca
      721 ccagcgcacc agcattaata aacggattac gcggaatacc ctgttccatt tccagttgca
      781 ctaaagaatt gaacggtgat ccagacggat ctttgccgac gcgttgccag atttcctctt
      841 cggagtaatg acgcatggcg acaacgagac tcagcacttt ggaaatagac tgaatggaaa
      901 aacgttcttg cgcgtctccg gcctgaaaaa gctgtccgtc aacggtacag atagcaatgc
      961 ccaatcggga accgtctact gtagccagcg ccggaatata atccgcgact ttaccctgac
     1021 caatgagcgg ccgcacttgc cgcaagatgt tttctaaaat tgcattatcc atggcgactg
     1081 ccactttcta ctcctggacc gcaggtctga aaagacctgc gagtatatca gagctgaata
     1141 tgtcgcgtca gatccggtct ttccacaccg tctggatatt acagaattcg tgtaagccga
     1201 aatgggaaag ctcacgacca aagccactct ttttcacgcc accaaaggcc actcgcgcgt
     1261 cgctggcaca ataaccattg ataaacaccc caccgcattc cagacgtgcc gccatctgtc
     1321 tggcctgtgt ttcgtcggtg gtaaaaatgg tcgctgaaag gccgaactca ctatcattag
     1381 ccagttccag cgcatgttct gcatctttcg caacggtgat tgccgcaacg gggccaaaca
     1441 tttcttcccg aaacgcggtc atttctgggg taacattcgc cagaaccgtt ggcggatagt
     1501 agttacctgc cccagccatc ttttccccgc ccagtaacaa acgcgcaccc tgcgccaggg
     1561 ttttctctac ctgatgatgc agctcatcac gtaaatcaaa acgagccatt ggtccgagag
     1621 tgttctcttc gtcacgggga tcgcccattt tcaaggctgc cgcagctgcc acaaaacgtt
     1681 cggtaaatgc agaagcaatt ccctcttcga taataaagcg ttttgccgct gcacatacct
     1741 gtccggtatt ctgataacgt ccggctaccg ccgctttaac cgccagttcc agatcggcat
     1801 cgttaagcac aataaacgga tccgaaccgc ccagttccag tacgcatttt ttcagtgccg
     1861 ctccagcctg tgcgccaata gccgctccag cacgaacact tccggtcacc gtgacagcag
     1921 caatgcgcga gtctttaatc atctgactga caccgtcgtt gtcggcattc agccagccat
     1981 atacgccttg cgggattccc gcatctttaa acacctgggc aatgagctga gcacatccca
     2041 tcacattcgg cgcatgttta agtaagtagc cgttacctgc aagaatgatg ggaacagcgc
     2101 cacgcatcac ctgccataac ggaaaattcc acggcataat cgccagaatc gtccccaacg
     2161 gtcgatactc aataaccgcc tgctgatttt ccaccagtgt aggttccgcc ttcagcattg
     2221 ccggaccatg ttctgcatac cagtcacaca aattcgccga tttcgccact tcagcgcgcg
     2281 cctggttgat tggtttgccc atttcgcggg tgatcatttg cgccatttct tcgctacggg
     2341 cgcgcagagc cttaccgata tcacgcattt tttcagcacg ataatctata tttgtctcgc
     2401 gccagtcgcg aaagcctgct gccgccagct caagtgcgtt ttcgatatcg ttagcgccag
     2461 cccacggcag cacagaaagt tgttcacccg tggcaggatt tattgaaatt gcatgagttg
     2521 ccggagtaat ggtcatcggg gtatctcctt tatgagtcat ggtatgaaga tacgcagatt
     2581 tactcttgct ttaaaatgaa taatattaag ccacttattc acgaatcgag aatgttatgg
     2641 atctgaccca actggagatg ttcaacgcgg ttgccgaagc tggcagcatt acccaggctg
     2701 cagcaaaagt gcatcgcgta ccgtcgaatc tcactacccg tctacgccag ctggaaacag
     2761 aactgggggt agatctgttt attcgcgaga atcagcgttt acgtctctct cctgccgggc
     2821 ataacttttt acgctatagc caacaaattc tcgcgttagt ggatgaagcg cggagcgttg
     2881 tcgctggcga tgagccgcaa ggtttatttt ctcttggttc gctggaaagc accgcagcag
     2941 tgcgcattcc ggccacgctg gcggaattca accatcgtta tcccaaaatt cagttttcac
     3001 tttccaccgg cccttccggc actatgctgg aaggagtgct ggagggaaaa ctgaatgcgg
     3061 catttattga tgggcccatt aaccatactg ccattgacgg gataccggta taccgcgagg
     3121 agctgatgat cgtcacgcca caaggtcatg cgccagtaat ccgcgccagt caggttaacg
     3181 gcagtaacat ttatgccttc cgcaccaatt gttcgtatcg tcgccacttc gagagctggt
     3241 ttcatgctga cggtgccgct ccgggaacta ttcacgagat ggagtcgtat cacgggatgt
     3301 tggcctgtgt ggtcgcagga gcaggcattg cgcttattcc acgctctatg ctggaaagta
     3361 tgccggggca tcaccaggtt gaagcgtggc cgttagctga gcaatggcgt tggttaacaa
     3421 cctggctggt ctggcgtcgc ggtgcgaaaa cgcgtccgct tgaggcattt attcaactac
     3481 tggatgtgtc tgactcggca aaacagagtt accaatgaac tattttgata attcttgcgg
     3541 ctaatatgct ctatatagtg atgttccgat gacttatgac tatatggggc aaatatggtt
     3601 acgccagtaa gcatcagcaa ttacatatct cttcctgatg attttcccgt tcgtaatatt
     3661 gcaccacagg taaaagaagt tttaaaggac tttattgatg cgctaagtac aataatatgt
     3721 gatgaagagt ggcgcacgtc tttaaacatc aactcagcca caaaaaagat atttaataac
     3781 cttgataact tatcatatat tcagaggact tctttccggg gtaacgacac gctatacaat
     3841 gaaaaggttc agtttaaact cacttatccc gtcaagaatg gaagacacaa agaaaatatt
     3901 gaatttcagg tagtaataaa tttaagccct atttatttag ataattttcg ccatgatgga
     3961 gaaattaata ttttttgcgc tcccaaccca aagcctgtca ctatggggcg cgtatttcag
     4021 accggcgtcg agcgtgtgct ctttctgttt ctgaatgatt ttattgaaca atttccaatg
     4081 ttcaaccctg gcgttccgat caaaatagcg catacaccac atattgaacc cctgcctccg
     4141 gatcaccata ccgctgcaga ttacttacgc cagtttgatt tgcttgtcct gaattttatc
     4201 tctcgcggta attttgtcat actcccccga ttatggaata actctgaggt tcacagatgg
     4261 ttcgtcaata aagatcctaa cttgatcacc gccattctcg acataacgga cagcgaatta
     4321 aaagaggatt tattgcaaag cctgatggat tcattaggtt ctaacaaaca tgtactaccc
     4381 gaagtctgca tctgcttttt attcctttta gcagaacaag aatctcctca ttttcaggac
     4441 ttgtttttat ttttcgccaa tatgttattg cactatcacc aatttatgaa tcccaatgaa
     4501 agtgatttga atgacgtgtt gatgccagca tcattaagtg atgataaaat tatcaagcat
     4561 atggcgcgca ggaccctcga actgtttgta aaaaatgaaa caccgccaaa agttactcac
     4621 gaagacctgg tgaaaaacag gcctcgctcc cctgtcagac cacctatacc cgcaacctcc
     4681 aaaacgccag acctccctga acgtcattaa accgtgatgt taccgactct ctgacgcgga
     4741 acagaatcag cgtcagagaa acggaaaacg cgatccagat cacaaataca ttgtattcac
     4801 atcattaacc gttttaagat catttcatca ctttttcgca actcacccga taatctgtta
     4861 tgacaacaaa cactgtttcc cgcaaagtgg cgtggctacg ggtcgttacg ctggcagtcg
     4921 ccgccttcat cttcaacacc accgaatttg tccctgttgg cctgctctct gacattgcgc
     4981 aaagttttca catgcaaacc gctcaggtcg gcatcatgtt gaccatttac gcatgggtag
     5041 tagcgctaat gtcattgcct tttatgttaa tgaccagtca ggttgaacgg cgcaaattac
     5101 tgatctgcct gtttgtggtg tttattgcca gccacgtact gtcgttttta tcgtggagct
     5161 ttaccgttct ggtgatcagt cgcattggtg tggcttttgc acatgcgatt ttctggtcga
     5221 ttacggcgtc tctggcgatc cgtatggctc cggccgggaa gcgggcacag gccttgagtt
     5281 taattgccac cggtacagca ctggcgatgg tcttaggttt acctctcggg cgcattgtgg
     5341 ggcagtattt cggttggcga atgaccttct tcgcgattgg tattggggcg cttatcaccc
     5401 ttttgtgcct gattaagtta cttcccttac tgcccagtga gcattccggt tcattgaaaa
     5461 gcctcccgct attgttccgc cgcccggcat tgatgagcat ttatttgtta actgtggtgg
     5521 ttgtcaccgc ccattacacg gcatacagct atatcgagcc ttttgtgcaa aacattgcgg
     5581 gattcagcgc caactttgcc acggcattac tgttattact cggtggtgcg ggcattattg
     5641 gcagcgtgat tttcggtaaa ctgggtaatc agtatgcgtc tgcgttggtg agtacggcga
     5701 ttgcgctgtt gctggtgtgt ctggcactgc tgctacctgc ggcgaacagt gaaatacacc
     5761 tcggggtgct gagtattttc tgggggatcg cgatgatgat catcgggctt ggtatgcagg
     5821 ttaaagtgct ggcgctggca ccagatgcta ccgacgtcgc gatggcgcta ttctccggca
     5881 tatttaatat tggaatcggg gcgggtgcgt tggtaggtaa tcaggtgagt ttgcactggt
     5941 caatgtcgat gattggttat gtgggcgcgg tgcctgcttt tgccgcgtta atatggtcaa
     6001 tcattatatt tcgccgctgg ccagtgacac tcgaagaaca gacgcaatag ttgaaaggcc
     6061 cattcgggcc ttttttaatg gtacgtttta atgatttcca ggatgccgtt aataataaac
     6121 tgcacaccca tacataccag caggaatccc atcagacggg agatcgcttc aatgccaccc
     6181 ttgcccacca gccgcataat tgcgccggag ctgcgtaggc ttccccacaa aataaccgcc
     6241 accaggaaaa agatcagcgg cggcgcaacc atcagtaccc aatcagcgaa ggttgaactc
     6301 tgacgcactg tggacgccga gctaataatc atcgctatgg ttcccggacc agcggtactt
     6361 ggcattgcta gcggcacaaa ggcaatattg gcactgggtt catcttccag ctcttccgac
     6421 ttgcttttcg cctccggtga atcaatcgct ttctgttgcg gaaagagcat ccgaaaaccg
     6481 ataaacgcga cgattaagcc gcctgcaatt cgcagaccgg gaatcgaaat gccaaatgta
     6541 tccatcacca gttgcccggc gtaatacgcc accatcatga tggcaaatac gtacaccgag
     6601 gccatcaacg actgacgatt acgttcggca ctgttcatgt tgcctgccag gccaagaaat
     6661 agcgcgacag ttgttaatgg gttagctaac ggcagcaaca ccaccagccc caggccaatt
     6721 gctttaaaca aatctaacat tggtggttgt tatcctgtgt atctgggtta tcagcgaaaa
     6781 gtataagggg taaacaagga taaagtgtca ctctttagct aaccttgcat cgcattgacc
     6841 aaaacttgaa ccgatttagc aaaacgtggc atcggtcaat tcattcattt gacttatact
     6901 tgcctgggca atattatcct ctgcaactaa ttacttgcca gggcaactaa tgtgaaaagt
     6961 accagcgatc tgttcaatga aattattcca ttgggtcgct taatccatat ggttaatcag
     7021 aagaaagatc gtctgcttaa cgagtatctg tctccgctgg atattaccgc ggcacagttt
     7081 aaggtgctct gctctatccg ctgcgcggcg tgtattactc cggttgaact gaaaaaagtg
     7141 ttgtcggtcg acctgggagc actgacccgt atgctggatc gcctggtctg taaaggctgg
     7201 gttgaaaggt tgccgaaccc gaatgataag cgcggcgtac tggtaaaact taccaccagc
     7261 ggcgcggcaa tatgcgaaca atgccatcaa ttagttggcc aggacctgca tcaagaatta
     7321 acaaaaaacc tgacggcgga cgaagttgca acacttgagc atttgcttaa gaaagtcctg
     7381 ccgtaaacaa aaaagaggta tgacgatgtc cagacgcaat actgacgcta ttaccattca
     7441 tagcattttg gactggatcg aggacaacct ggaatcgcca ctgtcactgg agaaagtgtc
     7501 agagcgttcg ggttactcca aatggcacct gcaacggatg tttaaaaaag aaaccggtca
     7561 ttcattaggc caatacatcc gcagccgtaa gatgacggaa atcgcgcaaa agctgaagga
     7621 aagtaacgag ccgatactct atctggcaga acgatatggc ttcgagtcgc aacaaactct
     7681 gacccgaacc ttcaaaaatt actttgatgt tccgccgcat aaataccgga tgaccaatat
     7741 gcagggcgaa tcgcgctttt tacatccatt aaatcattac aatagctagt tgataacgtg
     7801 acaacgtcac tgaggcaatc atgaaaccac tttcatccgc aatagcagct gcgcttattc
     7861 tcttttccgc gcagggcgtt gcggaacaaa ccacgcagcc agttgttact tcttgtgcca
     7921 atgtcgtggt tgttccccca tcgcaggaac aaccaccgtt tgatttaaat cacatgggta
     7981 ctggcagtga taagtcggat gcgctcggcg tgccctatta taatcaacac gctatgtagt
     8041 tattgtcagg ctctgatcac agagcctgac atctccgtaa tatcttcctc aagtctcaaa
     8101 tattttcgcc attgttggcg atgagatccc gataccagta aaacgacgct ttacgtatcc
     8161 gttccagcgt tcccttccct tcgttgtctt tatcgacata aatcattccg tagcgctttt
     8221 tcatttcccc tgttccggca gaaaccaggt caatgcagcc ccacggggtg tagccaatta
     8281 agtcaacacc atcttcaact acggcttttt tcatttcccg aatatgggaa gcaaagtaat
     8341 caatgcgata gtgatcgttc accgtgccgt cagcttgtct ctggtcaacc gcaccaaatc
     8401 cattttcgac aataaacagc ggcaactgga aatgatccca gaaccagttg agtgaataac
     8461 gtagccctgc cggatcaatt tgccatcccc attcggatgt atcgatataa gggttgctga
     8521 ccaaatctcg tggttcaaca taatccagtt gcgggttatc gggagaaaat tgtgtcgtaa
     8581 aagacatgta gtagctaaaa ccgataaagt cgacacatcc actggcaaga atcgcgttat
     8641 cttctggtgt gatatcgagg ttgaatcctt tcctggcaaa gtaattcagc atatgttgcg
     8701 gataatatcc acgagcatga acatcagtaa accagtaacg acgatgcatc gctttcgtgg
     8761 ccatcatcat atcgttgggt gcacacgtca gaggatagat ggggcacatg gcgatcatac
     8821 agccaatgtt aaattcagga ttgatcgatt ttccagtctg taccgccagg gcactggcaa
     8881 ctaactcgta atgtaccgcc tggtacatta tttgttcgcg ctcattgata tcttcctctg
     8941 gcgaatacaa gataccggaa ttagtaaatg gacacaggct ttcgctgaaa ttcacctgat
     9001 tattgatttc gttaaacgtc atccagtact ttactttttc tttatagcgc gtgaagaccg
     9061 ttgatgcgaa gcggataaaa aagtcgatca gcttacggtt tcgccagcca ccatattttg
     9121 tcaccagatg ataaggcatc tcaaaatgcg aaagcgtcac cacaggttcc attccttgct
     9181 tcaggcattc atcgaacaga tcatcataaa attgtaaacc ctcttcattc ggctcctgtt
     9241 cgtcaccttg cggaaagatt cgtgtccagg caatggaagt tcgaaagcat ttgaatccca
     9301 tctcggcaaa taactgaata tctgttttat agcgatgata aaaatcaatt gcttcatgat
     9361 tgggataatt aagcccgtcg ataacgcctt ctgtcacttc acgcggcacc ccgtgagcgc
     9421 cagcagtcat cacatcagcg atactgatgc cttttcctcc ttcattccag ccaccttcca
     9481 actgatgcgc ggctaccgcg ccaccccata aaaaaccttt tttaaatcct gacatacaca
     9541 actccttaac taaaatagat taccaccaga cttccgcc
//
LOCUS       DAEEKQ010000146         9568 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.148, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000146 DAEEKQ010000000
VERSION     DAEEKQ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9568
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.148"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(204..989)
                     /locus_tag="KLK57_003325"
     CDS             complement(204..989)
                     /locus_tag="KLK57_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893617.1"
                     /translation="MNLRRVCCICVLFSLLAGCVSESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     FQDTLQTGKYDSGLKSIVWSDYKNQKLTDTMSLRYLVRFTLVDVATGEWAAWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
     gene            complement(1319..1395)
                     /locus_tag="KLK57_003326"
     tRNA            complement(1319..1395)
                     /locus_tag="KLK57_003326"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1359..1361),aa:Asp,seq:gtc)
     gene            complement(1528..2259)
                     /gene="dnaQ"
                     /locus_tag="KLK57_003327"
     CDS             complement(1528..2259)
                     /gene="dnaQ"
                     /locus_tag="KLK57_003327"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HBE0893618.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            2324..2791
                     /gene="rnhA"
                     /locus_tag="KLK57_003328"
     CDS             2324..2791
                     /gene="rnhA"
                     /locus_tag="KLK57_003328"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HBE0893619.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(2788..3510)
                     /locus_tag="KLK57_003329"
     CDS             complement(2788..3510)
                     /locus_tag="KLK57_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE0893620.1"
                     /translation="MKPARVPQTVVAPDRWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLRETDRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            3544..4299
                     /gene="gloB"
                     /locus_tag="KLK57_003330"
     CDS             3544..4299
                     /gene="gloB"
                     /locus_tag="KLK57_003330"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HBE0893621.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLNKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            4371..5729
                     /gene="mltD"
                     /locus_tag="KLK57_003331"
     CDS             4371..5729
                     /gene="mltD"
                     /locus_tag="KLK57_003331"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HBE0893622.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(5778..6374)
                     /locus_tag="KLK57_003332"
                     /pseudo
     CDS             complement(5778..6374)
                     /locus_tag="KLK57_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
     gene            complement(6405..7205)
                     /locus_tag="KLK57_003333"
     CDS             complement(6405..7205)
                     /locus_tag="KLK57_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HBE0893623.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            7446..8360
                     /locus_tag="KLK57_003334"
     CDS             7446..8360
                     /locus_tag="KLK57_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0893624.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(8357..9160)
                     /gene="dkgB"
                     /locus_tag="KLK57_003335"
     CDS             complement(8357..9160)
                     /gene="dkgB"
                     /locus_tag="KLK57_003335"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HBE0893625.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGHAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKAIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(9323..9399)
                     /locus_tag="KLK57_003336"
     tRNA            complement(9323..9399)
                     /locus_tag="KLK57_003336"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9363..9365),aa:Asp,seq:gtc)
BASE COUNT         2597 a         2253 c         2227 g         2491 t
ORIGIN      
        1 tattaaaaat ggcatgaaat gtaataatga atatatttct cgcaaggaag attcagcagt
       61 taaataatta aacattctaa aacaaacagt ttttaactcc ggattccatg tcctttcttg
      121 cactgtcttt gtcaagaaaa atttcttatg caaagaattt tgtattattt ccgccagaaa
      181 ttcctggcgg cagatataat ttattattga tagcggttaa ccaaatcttt taccatcgct
      241 ttataggtct tttgcttaag ttgcatgatt tgctgctctg tcatatcagt cgtactggct
      301 tcattcttgt cgggcaatgg tggaagcact ttatattcat aattcaccgg cgaccaggca
      361 gcccactcac ctgtagctac atccaccagc gtgaatcgta ccagatagcg cagtgacatc
      421 gtgtcagtca atttttgatt tttataatct gaccaaacta ttgatttcaa tccagagtca
      481 tattttcctg tctgcaacgt atcctggaaa acaataatcg ctttctgatg cccttttgca
      541 gccacaaaac gcagtgcctg catccagttc atattctcag cactggcaac atcctcgttc
      601 tcatttttat ctttgttttt gttacaagtc agaggctttt gcctgtccgg gataccagag
      661 aatgtggaaa cagtataata ttttcgcatc tcctcctgca taatggtttc cggtgcgcgg
      721 ttgccggatt gaacaagtat tacaggagaa ttaaggggaa cgcggaactc atctccatca
      781 agcgcagctt gaatatcctc ttcggatacg gccagagtgg tttcattacc gaataaatct
      841 ttgtctgtca gttgctgggg agtgttttta ttaatattac tttgtgtgac ttgagctttt
      901 ttcttctttt catcaataga agattcagag acacatcctg caagcagcga gaataataca
      961 catatacaac aaacccttct taaattcata atcggacact ccctcgcctg tcataatcat
     1021 ggtaatgttt agctaacgat ttttaattat atactccact tgaatcagaa caaacaaaaa
     1081 taacagtata tataaattca ttaaatcaat agaaaaatga aaatcataac attcaaatat
     1141 atttaaattg aatgatttga tagtatgatt aaatattaat gatatggtct accatatgtg
     1201 attaacacat ttacagcata acaaaaccat agaccgaaga aatagcggga tttaaatgca
     1261 aaaaaaccgc aaaattaaaa atcttgcggt tctctgaact cattttcatg agtgaatttg
     1321 gcggaacgga cgggactcga acccgcgccc ccctgcgtga caggcaggta ttctaaccga
     1381 ctgaactacc gctccgcgtt gtgttccgtt gggaacgagg cgaatattac ggattgcctc
     1441 acccttcgtc aacggttttt ctcatctttt gaatcgtttg ctgcaaaaat cgcccaagcc
     1501 gctattttta gcgcctttta caggtattta tgcccgccag aggcagcttc cgcccttctt
     1561 ctccaccaga tcaagacggg cttcatgagc tgcaagctct tcatctgtcg caaaaacaac
     1621 gcgtaactta cttgcctgac gtacaatgcg ctgaattgtt gcttcacctt gttgctgctg
     1681 tgtctctcct tccatcgcaa aagccatcga cgtttgacca ccggtcatcg ccagataaac
     1741 ttccgcaagg atctgtgcat cgagtaatgc cccgtgtagc gttcgtttac tgttatctat
     1801 ttcgtagcgg gcacataacg catcgaggct gttgcgctta ccaggaaaca ttttcctcgc
     1861 caccgcaagg ctatcggtga ccttacagaa ggtattggtc ttcggaatat cgcgcttaag
     1921 caacgaaaac tcgtagtcca taaagccgat atcgaacgct gcgttatgga tcaccaactc
     1981 cgcgccgcgg atatagtcca tgaactcatc ggctacttcg gcaaacgtgg gcttatcgag
     2041 caaaaattca tcggcaatac catgtacgcc aaaggcttcc ggatccacca gccgatcggg
     2101 tttgagataa acatggaagt tattgcccgt caggcgacgg ttcaccactt caacggcacc
     2161 aatctcaatg atcttgtggc cttcatagtg cgcaccaatc tggttcatac cggtggtttc
     2221 ggtatcgaga acgatctggc gtgtaattgc agtgctcata gcggtcattt atgtcagact
     2281 tatcgtttta cagttcgatt caattacagg aagtctacca gagatgctta aacaggtaga
     2341 aattttcacc gatggttcgt gtctgggcaa tccaggacct gggggttacg gcgctatttt
     2401 acgctatcgc ggacgcgaga aaacctttag cgcaggctac acccgcacca ccaacaaccg
     2461 tatggagttg atggccgcta ttgtcgcgct ggaggcgtta aaagaacatt gcgaagtcat
     2521 tttgagtacc gacagccagt atgtccgcca gggtatcacc cagtggatcc ataactggaa
     2581 aaaacgtggc tggaaaaccg cagacaaaaa accagtaaaa aatgtcgatc tctggcaacg
     2641 tcttgatgct gccctagggc aacatcaaat caaatgggaa tgggttaaag gccatgccgg
     2701 acacccggaa aacgaacgct gtgatgaact ggctcgtgct gcggcaatga atcccacact
     2761 ggaagatacg ggctaccaag tcgaagttta agcttgtggt ttacgacatt gccgggtggc
     2821 tccaaccgcc tggcgaattc gtggcttgtt tttactctgt ttcatcgggt ttagcgttaa
     2881 aggaatagtc cgtttccggg caacaataag ttgtaagcaa ccaagcgcag gaatatgcgc
     2941 attcaatagt tttcctccgt gtttgttcca cgggagaacg tggaaacggc tggcgtgcag
     3001 cacttcaaaa ttcaacaaag agagccagtc cagttgccgc atcagagtaa acatccggct
     3061 gttatagggc gaggtttttc gcaataccgg cacaagtttg cgtaatccca ttaaactaat
     3121 gggattgaag ccactaatca ccagccagcc atcatcaatc aatacccgat cggtttcacg
     3181 caataaacga tgcggatcgg tgcaccacgg caatgtatgt gccagtagac aaacatcaac
     3241 ggatttatcg gcaaaaggaa gatgaagtgg atccgcctgc acctggacgg gcattccttg
     3301 cgcagaaaca ttcacttgat gagaaaccgc gcacgcttcg caattgattt ctgcgcttaa
     3361 attgccaatc ttaagcagat gaaaaccata cattttagtg aaccacgggt tgagctggcg
     3421 ctccagcgcc ttgcgataaa gctctcccca gggcaaatcg ccccagcgat caggagccac
     3481 gacagtttga gggactcttg ccggtttcat cacaaccttc cgtttcacac taagaggtaa
     3541 tctatgaatc ttaacagtat tcccgccttt gatgacaatt acatctgggt tttgaatgat
     3601 gaagcaggtc gctgcctgat tgtcgatccc ggagacgcag agccagtatt aaacgccatt
     3661 gccgccaata actggcaacc ggaggccata tttctcaccc accatcatca cgatcacgtt
     3721 ggcggcgtaa aagaactggt ggaaaagttt ccgcaaattg tggtgtatgg tccacaagag
     3781 acacaagata agggaacaac acaggtagtc aaagatggcg aaactgcctt cgttttgggg
     3841 catgaattta gtgtaattgc cacaccgggt cacactttag gacatatctg ttacttcagt
     3901 aaaccttatc tattttgcgg cgacacgctg ttttctggtg ggtgtggtag gttgtttgaa
     3961 gggacagcat cacaaatgta tcaatcactt aataagttaa gtgcgttacc tgacgataca
     4021 ttggtatgtt gtgctcatga atatacgtta tcaaatatga agtttgcttt gagtattctt
     4081 ccgcacgatt tgtccataaa tgattattat cgtaaagtta aggagttacg ggcaaaaaat
     4141 caaataacac tacccgtaat tctgaaaaat gagcggcaaa ttaatgtttt tttaagaacg
     4201 gaagatattg atttaattaa tgtaattaat gaagaaacat tattgcaaca acctgaagag
     4261 cgttttgcat ggttaaggtc aaagaaagat aggttctgat aaaactttct tgtcatcggc
     4321 tccgttcgcc gttatgatcg gtcgtctttt aagcaactat tgacacacac atgaaggcaa
     4381 aagcgatatt actcgcctct gtcctgctcg tgggttgcca gagtaccggc aacgttcaac
     4441 agcacgcaca gagcctttct gcagctggtc aaggggaagc agcaaagttt acaagtcagg
     4501 cacgatggat ggacgatggg acgtctatcg cgccagatgg tgacttgtgg gctttcattg
     4561 gcgacgagct aaagatggga attccggaaa atgaccggat tcgcgaacag aaacagaaat
     4621 atttacgcaa taagagctat ctccacgatg taactttacg ggcagagccg tatatgtact
     4681 ggatagcagg gcaagttaaa aaacgtaaca tgcctatgga actggtacta ctacccatag
     4741 tggagagcgc ttttgatcct cacgcaacgt ctggcgccaa tgccgcaggc atctggcaga
     4801 tcattccgag cacggggcgc aattatggtt tgaaacagac ccgcaattat gacgcgcgtc
     4861 gcgatgttgt tgcttcaaca actgccgcgc tgaatatgat gcagcgtctg aacaaaatgt
     4921 ttgacggcga ctggcttctg accgtagcgg cttataacag cggcgaaggt cgggtcatga
     4981 aggcaattaa aacgaacaaa gcgcgtggga aatccacgga cttctggtcg ttaccgttgc
     5041 cgcaggaaac gaagcagtac gtgcctaaaa tgctggcatt gagtgatatt ctcaaaaaca
     5101 gcaagcgtta tggcgtacgt ctgccaacga ccgatgaaag ccgtgctctg gcgcgtgtgc
     5161 acctgagtag cccggttgaa atggcgaagg ttgcagatat ggcggggatt tccgtcagca
     5221 agctgaagac attcaacgct ggcgtgaaag gctccacgct gggcgcaagt gggccgcagt
     5281 acgtgatggt gccaaagaag catgcagatc aactgcgtga atctctcgct tcaggcgaaa
     5341 ttgctgctgt acagtcgacg ctggttgccg acaatacgcc gcttaacagc cgtgtttaca
     5401 ccgtacgctc tggcgacacg ctttcaagca tcgcttcacg tctcggcgta agcaccaaag
     5461 atttgcagca gtggaacaaa ctgcgcggat ctaagctgaa gccaggccaa agtttgacga
     5521 ttggtgcagg cagtagcgca cagcggttgg caaacaacag cgatagcatt acgtatcgtg
     5581 tgcgcaaagg cgattcgctt tcaagcattg ctaaacgcca cggcgtgaac atcaaagatg
     5641 tgatgcgctg gaacagcgac actgcgaatc tgcaaccagg cgataagctg acgttgtttg
     5701 tgaaaaacaa caacatgcca gattcctgac aaaccagata ataaaaaagg caccgattcc
     5761 cccggtgcct tttttattta tgccgcttta tgtgcttcta ccatgatgat atcactggtg
     5821 aaagagccat cagtctgcaa ggaaaaatac gttctcacct ctgtcgatgc gctctgttgg
     5881 taaatgcgaa tagcgtctac taacgcttct ggcgtacgca ttctcacgac ccatgaagaa
     5941 aattccagcg gtaacttatc tgtaattaaa ttatcaacta tcagattggc ttcattgatt
     6001 aacgtcaacc acgcaccgct ggcgtagttt cgtacgtgag aggtatcgcg taatgcttct
     6061 accgcctgta accagatgtc gcgcactggg tgacccggag acattacatc catcacaatc
     6121 agcctaccgc caggtttcaa tatcctattc acttctcgca gtgctgcacc aacatcatgc
     6181 cagtgatggg cagaataacg gctgataaca atatcaaatg cgttatcggc aaatggcaga
     6241 cttgcggcat atccctggcg ggtggtgata tttttcagtt gccgggcttc ggcggcttgt
     6301 gccacgacat ccagcatgtg ggcagataag tcatacgcca ccaccgcgct cacgttttgc
     6361 gcagcgccaa acgttggtct gccctgcggc agacctgaca taccttattt atcaggcttg
     6421 ccgggactga attcaacgag tagcggattg tgatcggaag cgcgcgtaac cagtacagaa
     6481 gcttcactga cgttcagacc acggtagaaa acaaaatcga gcgggcgacc aaacgcccga
     6541 cggcgctgat catcggtaaa acgcacctgg cgcagcgaca tttcccgcgc aaagcgatat
     6601 aacgcgttca tccttctacg gctccaggca ttgaaatctc ccgccataat gaccgggccg
     6661 ctgtggtgag ctatctgatc accaatagga agtaactgct tactatagac atccacgccc
     6721 agactgaagt tgacggcgtg tatattaacc accatcaaca ggcgggtgtc aggtaatgga
     6781 tagaccgtca ccagtgccga cttcgccaga cgcaaaatgg gttctcgttc acgtaacggg
     6841 cagcaataca ctggatgtgc cgccgaaagg gtcattacgc cagaaggatg ttgtggcagc
     6901 acgaaagcgg gtacctgatc ggcggcaaga tagttagcgg tcgcaaactg taccaactct
     6961 ggcgttgttt gcgcttcctg caataacacc agatgtgcgt ctttgccgta attctttaat
     7021 accgacaacc attcagcgcg ttgctgtttg tatatgttcc acaccaggat ccgaatacgc
     7081 tcttcggtac ttaacggttc tccgggtggt aatgcctggc cgatgctcgc aaaagacccc
     7141 ggcggtaaga tcctctccgc aggttgtccg gcaacatagc gcatggcata ggtgtttttt
     7201 cgcacttttg actttgaaac ctctattacg tcaacccttc cgcactcggg agggttctac
     7261 tgttataggg attttagctc actctttcaa cgagcaattg tatattgtta tgtaagcaag
     7321 tgcttacacc ggtcagaaat ttgctcatta ttgagaacat taaatcggat gacatatcac
     7381 gcatcctgat ggtttaatga caaataaatt caaaatatct catctatttt gcaacaggag
     7441 caaaaatgaa agccacgtcg gaagaactcg ccatttttgt ttcggtcgtc gaaagcggca
     7501 gctttagccg ggcagcggaa caattagggc aagcaaactc agcggtaagc cgggcggtga
     7561 aaaagctgga aatgaaactt ggcgttagcc tgcttaatcg gaccacgcgg caacttagcc
     7621 tgacggaaga aggcgagcgt tattttcgtc gcgtacagtc aattttgcag gagatggcag
     7681 cggcagaatc agaaattatg gagacgcgta atacaccgcg tggactgtta cggatcgatg
     7741 ccgcaactcc agtggtgctg cactttctga tgccgttaat taagcctttc cgtgaacgct
     7801 atccggaagt cactttatcg ctagtctcct ccgaaacgat tattaatttg atcgaaagaa
     7861 aagtggatgt cgcgatacgc gctggtacgt taacggactc cagcttacgt gccaggccgt
     7921 tatttaacag ttatcgaaaa attatcgcct cccccgatta tatttcccgc tacgggaagc
     7981 cagaaacgat cgacgattta aagcaacatg tttgcctggg attcactgaa cccgcttccc
     8041 tcaatacctg gccgatagcc tgtagtgatg gacaattaca tgaggtgaag tacggtttgt
     8101 catccaatag tggggaaaca ctgaaacagc tttgcctgag tggcaacggg attgcgtgtt
     8161 tgtccgacta catgatcgac aaagaaatcg ctcgcggaga attggtggag ttaatggcag
     8221 ataaagtgtt gccagtggaa atgccattca gtgccgtcta ttacagcgac cgtgcggtaa
     8281 gtacgcgcat ccgggctttt atcgatttcc ttagcgagca tgtaaaaaca gctcccggag
     8341 gagctgtcag agaggcttaa tcccattcag gagccagacc ttccgggcta accaggcggt
     8401 cgttgcaatc cagtgcggcg atcgcttttt tatcttcggc atcaagctgt aaattttgtg
     8461 ccttaagatt actttccagg tttttacgtt tagtagaaga aggaattact gagtaacctt
     8521 cccccatagc ccacgccaga atcacttgtg ccggagtcgc attgtgttta gctgcaatac
     8581 gagcaataac ctcatctttc agtgccttac cataagccag cgtcatatag gaagtgatat
     8641 gaataccgtg ctgtttagcc caggcaacca ctttacggtt ttgcagataa ggagagagtt
     8701 caatctggtt ggtagcgatg ttttcagcgc caacagcagc aatcgctttt tccatcaacg
     8761 ggatcgtgaa gttggaaata ccgatctcac gtgtcagacc ttgttttttg gcctccagca
     8821 gcgcctgcat aaactcttca acagagactt catcgtttgg tgacggccag tggattagcg
     8881 tcagatcaac ataatcggta cgcaattttt gcaggctctc ttttagactc gggatcaatt
     8941 tgtctttgct gagattttca atccagattt tagtggtgat gtagagttca tgacgtggca
     9001 cgccactttc tgcaatcgca tgacctactg cggcttcgtt atcatagatt tgtgcagtat
     9061 caattgcgcg ataaccaagt tcaagcgccg ttttcacaga tgaaataaca acgtcgtctt
     9121 tgagacggaa agtacctaaa ccaaatgcag ggatagccat aatatgcctc ttttaatgct
     9181 attttcgtta actgaggtga ttatgggctt cagactgttg cggaaaaaga gcataaaatg
     9241 cagaggattt ttgcgattct ggcaataata gatatacaga cgaaaaaaaa cctcgagcgt
     9301 taactcgagg cttcttaata agtggcggaa cggacgggac tcgaacccgc gaccccctgc
     9361 gtgacaggca ggtattctaa ccgactgaac taccgctcca ccgaattctt ttacaaccac
     9421 cggttttatg accggcttac tgcttaattt gatgcctggc agtttatgaa tcacttcggg
     9481 ctgttgcttc gcaacgttca aaagctttcg cttttgtcct actctcttat cgagtagtga
     9541 actgcctcaa aggtcagtgc cattcttc
//
LOCUS       DAEEKQ010000147         9542 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.149, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000147 DAEEKQ010000000
VERSION     DAEEKQ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9542)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9542)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9542
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.149"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..1321
                     /locus_tag="KLK57_003337"
     CDS             <1..1321
                     /locus_tag="KLK57_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HBE0893626.1"
                     /translation="PQFRIPAELIQHDIDFVAAHGVKFEYGCSPDLTVEQLKNQGFHY
                     VLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVVVVGAGNTAMDCAR
                     AALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNPERFDADGTLTLRV
                     MSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMGVPLDKNGWPDVDH
                     NGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSHQNDKYWNNVNPAE
                     IYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCNKCVDVCPNRANVSIAVPGFQN
                     RFQTLHLDAYCNECGNCAQFCPWNGKPYEDKITVFSLAQDFDNSSTPGFLVEDCRVRV
                     RLNNQSWVLNIDSEGQFNNVPPELNDMCRIISHVHQHHHYLLGRVEV"
     gene            1324..2652
                     /gene="ssnA"
                     /locus_tag="KLK57_003338"
     CDS             1324..2652
                     /gene="ssnA"
                     /locus_tag="KLK57_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417355.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HBE0893627.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSELSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARLIDEAKKATSEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDIALL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            2703..3482
                     /locus_tag="KLK57_003339"
     CDS             2703..3482
                     /locus_tag="KLK57_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HBE0893628.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYITGVLVADLYADCQQAGEEAV"
     gene            3479..6418
                     /locus_tag="KLK57_003340"
     CDS             3479..6418
                     /locus_tag="KLK57_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000583599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HBE0893629.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLDVIGKHYPKTDAAKMVQAKPCYVEDCVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGAPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGIGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDTCGIWAEESQSGHGNGYRCRNNFQHAANAERSLQSVAIT
                     RLSPLGI"
     gene            6351..6665
                     /locus_tag="KLK57_003341"
     CDS             6351..6665
                     /locus_tag="KLK57_003341"
                     /inference="COORDINATES: protein motif:HMM:NF019581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBE0893630.1"
                     /translation="MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGN
                     FLPGKEADFVVMEPTATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYE
                     RN"
     gene            6701..7051
                     /gene="ghxQ"
                     /locus_tag="KLK57_003342"
                     /gene_synonym="ygfQ"
                     /pseudo
     CDS             6701..7051
                     /gene="ghxQ"
                     /locus_tag="KLK57_003342"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
     gene            complement(7120..7608)
                     /locus_tag="KLK57_003343"
     CDS             complement(7120..7608)
                     /locus_tag="KLK57_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HBE0893631.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="KLK57_003344"
     CDS             complement(7608..9527)
                     /gene="ygfT"
                     /locus_tag="KLK57_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HBE0893632.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRIEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
BASE COUNT         2301 a         2559 c         2488 g         2194 t
ORIGIN      
        1 tcctcagttc cgtattcctg cagagttaat tcagcacgat atcgattttg ttgccgctca
       61 cggcgtgaaa tttgagtatg gctgctcacc cgatttaacc gttgaacagt taaaaaatca
      121 gggcttccac tatgttctga ttgccaccgg cactgataaa aatagcggtg tgaaactggc
      181 gggcgacaac caaaatgtct ggaaatcact ccccttcctg cgtgaataca acaagggtac
      241 agcgctcaag ctgggcaaac atgtggtcgt tgtcggggcg ggtaacaccg caatggactg
      301 cgctcgtgcg gcgttacgcg ttccaggcgt agaaaaagca acgatcgttt accgtcgttc
      361 actacaagag atgcccgcat ggcgcgaaga gtatgaagaa gcgttgcacg acggcgtaga
      421 gttccgtttc ctgaataatc cggaacgttt cgatgctgat ggcaccttaa ccttgcgcgt
      481 tatgtcgctt ggcgaaccgg atgagaaagg tcgtcgccgt ccggttgaaa ccaacgaaac
      541 agtaacactg catgtagaca gcctgatcac cgccattggt gaacagcagg atactgaagc
      601 cctgaatgcg atgggcgtgc cgctggacaa aaacggctgg ccagacgtcg accataatgg
      661 cgaaactcgt ctgactgacg tctttatgat cggcgacgta cagcgcggac catcctccat
      721 tgtcgctgct gtcggaaccg cgcgtcgggc gaccgatgcc atccttagtc gggaaaatat
      781 ccgttcccac cagaacgata aatactggaa caacgtcaat ccagcggaaa tctatcaacg
      841 taaaggcgat atctctatca cgctggtgaa cagtgacgat cgtgacgcgt ttgtcgccca
      901 ggaagccgct cgctgcctcg aatgtaacta cgtttgcaac aagtgtgtgg atgtctgccc
      961 gaaccgcgcc aacgtctcca ttgcagtccc tggcttccag aatagattcc agacgctgca
     1021 cctcgacgct tactgtaacg aatgcggcaa ctgcgctcag ttctgtccgt ggaacggtaa
     1081 accatacgaa gacaaaatca ccgtcttcag cctggcgcaa gactttgata acagcagcac
     1141 cccaggcttc cttgtggaag attgccgggt acgagtacgt ctgaataacc aaagctgggt
     1201 gttaaacatc gacagcgaag gtcagtttaa caacgtacca ccggagctga acgatatgtg
     1261 ccgcatcatc agccatgtcc accagcatca tcattatctg ctgggccgcg tggaggtgta
     1321 atcatgttga ttctgaagaa tgtcaccgca gtgcagttac acccggcgaa agtgcaggaa
     1381 ggcgttgata tcgctatcga aaatgacgtg attgtcgcta tcggcgatgc cctgacgcaa
     1441 cgctaccccg atgccagcta caaagagatg catggtcgga ttgtgatgcc gggaattgtc
     1501 tgctcgcaca accattttta ctcggagctt tcccgcggaa ttatggcaaa catcgcccct
     1561 tgcccggatt tcatctcaac gctgaaaaat ctctggtggc ggctcgatcg cgcccttgat
     1621 gaagagtcgc tctattacag cggactgatt tgttccctgg aagcgattaa gagcggatgt
     1681 acatcggtta tcgatcacca tgcctcgccg gcgtatatcg gcgggtcgct ctccacattg
     1741 cgcgacgcat ttttaaaagt tggcctgcgc gcgatgacct gttttgaaac tactgaccgt
     1801 aacaacggca tcaaagagtt gcaggaaggt gtagaagaaa acatccgttt cgcccgtttg
     1861 attgatgagg cgaagaaagc gacaagcgag ccgtatctgg tggaagcaca tatcggtgct
     1921 cacgcgccgt ttaccgtgcc ggatgccggt ctggagatgc tgcgtgaagc cgtgaaagcc
     1981 acaggccgtg gtttgcatat tcacgctgcg gaagaccttt acgacgtttc ctacagtcac
     2041 cactggtacg gcaaagacct gctggcacga ctggcgcaat tcgacctcat cgacagcaaa
     2101 acgctggtcg ctcatgggct gtacttgtcg aaagatgaca tcgccctact caatcagcgc
     2161 gatgcgttcc tggtgcataa cgcccgttca aacatgaaca accatgtcgg ctacaaccat
     2221 caccttagcg acatccgcaa cctggcattg ggaacggacg gcattggttc ggacatgttt
     2281 gaagagatga aatttgcctt ctttaaacat cgcgatgcgg gtggtccgct gtggcctgac
     2341 agttttgcca aagccctgac taacggtaac gaactgatga gccgcaactt tggcgcgaaa
     2401 tttgggcttc tggaagccgg ttacaaagct gatttgacca tttgcgatta caactcgccg
     2461 acgccgctgc tggcagacaa tatcgccggg catatcgctt tcggtatggg ctcaggcagc
     2521 gttcacagcg tgatggtcaa tggtgtgatg gtctatgaag accgtcagtt taacttcgat
     2581 tgcgattcca tttatgcaca agccagaaaa gccgctgcca gtatgtggcg tcggatggat
     2641 gcgctggcat aaatgacaga tgccctcttc ccgcagggaa gagggttaaa taaggaaaga
     2701 agatgattga acaatttttc aggcccgact ctgtcgaaca ggcgctggaa ctgaagcgcc
     2761 gctaccagga tgaagccgtc tggttcgccg ggggcagcaa actcaatgct acaccaaccc
     2821 gtaccgataa aaagattgcc atttccttac aggatctgga actggactgg gttgactggg
     2881 ataacggtgc actgcggatt ggtgcaatgt ctcgcttgca gccactgcgt gatgcgcgct
     2941 ttattcctgc agcactgcgt gaagccctcg gttttgttta ctcacgccat gttcgtaatc
     3001 agtcgaccat tggtggtgaa atcgccgccc ggcaggaaga gtcagtgctg cttcccgtcc
     3061 tgctagcact ggatgctgaa ctggtttttg gcaacggcga aacgctgtca atcgaggact
     3121 acctggcctg cccatgcgat cgcctgctaa ccgaaattat cattaaagat ccgtatcgca
     3181 cctgtgcgac ccgcaaaatt agccgttctc aggcaggttt aaccgtcgtg acggcagccg
     3241 ttgcaatgac agaccacgac ggtatgcgaa ttgcgctgga tggcgtcgcc agtaaagcac
     3301 tgcgtctgca cgatgtcgaa aaacaaaatc tggaaggcaa tgcacttgaa caggctgtcg
     3361 ccaacgccat tttcccgcag gaagatttgc ggggcagcgt ggcctataaa cgctatatca
     3421 cgggagttct ggtagccgac ctgtatgccg actgccaaca ggctggggag gaagccgtat
     3481 gatcatccac tttactttaa atggcgcgcc tcaggagcta accgttaatc caggcgaaaa
     3541 cgtgcaaaag ctgttgttta acatgggaat gcactctgta cgcaacagtg atgatggctt
     3601 cgggtttgcc ggttctgacg caataatctt taacggtaat atcgttaacg cgtccttgct
     3661 tattgccgca cagttagaga aggcagatat tcgtaccgca gaatctctgg gtaaatggaa
     3721 cgagttaagt ctggttcaac aggcaatggt tgatgttggc gtggtgcagt ctggttataa
     3781 cgatccagct gcagctctga ttatcaccga tcttctcgat cgcatcgccg cacctacccg
     3841 cgaagagatc gacgacgcgc tttctggttt gttcagccgc gatgcaggct ggcaacaata
     3901 ctatcaggtc attgaactgg cggttgcacg taaaaataat ccgcaggcca ccattgatat
     3961 cgctccgact ttccgtgacg accttgacgt cattggcaag cattatccta aaactgatgc
     4021 cgcgaaaatg gtgcaggcga aaccctgcta tgttgaagac tgcgtaacgg ctgacgcctg
     4081 cgtcattaaa atgttacgta gcccacacgc tcacgcactg attactcatc tggatgtcag
     4141 caaagctgaa gccttaccgg gcgtcgttca cgttattact cacctgaatt gccctgatat
     4201 ctactatacc ccggggggtc agagcgcacc ggaaccgtca ccgcttgacc gccgtatgtt
     4261 cggcaagaaa atgcgtcacg tcggcgatcg tgttgctgcg gtcgtcgcag aaagtgaaga
     4321 aattgcgctc gaagcactga agctcatcga cgttgaatat gaagtgctta agccggtaat
     4381 gtcgatcgac gaagcaatgg cggaagatgc gcctgtcgtg cacgatgaac cggtggtgta
     4441 tgttgctggt gcgccagata ctctggaaga cgataacagc catgcagccc agcgcggcga
     4501 gcatatgatc atcaacttcc cgatcggttc tcgccctcgc aaaaatatcg ccgccagtat
     4561 tcatggtcat attggcgata tggacaaagg ctttgccgat gccgatgtga tcattgagcg
     4621 aacctataac tcaacgcagg cacagcagtg cccgactgaa acacatatct gctttacccg
     4681 gatggacggc gatcgtctgg ttatccacgc ctccacccag gtaccatggc acttacgccg
     4741 ccaggtcgcg cgcctcgtgg gcatgaaaca gcataaagtt catgtcatta aagagcgagt
     4801 tggcggcggt tttggttcca aacaggacat cctgctggaa gaagtgtgcg cctgggcaac
     4861 ctgcgtgacc gggcgtccgg tactattccg ctacacccgt gaagaagagt ttattgctaa
     4921 cacctctcgt cacgtcgcga aagtcaccgt caaactggga gcgaaaaaag atggtcgcct
     4981 gacggcagtg aagatggatt tccgcgccaa cactggccct tacggcaacc actcactcac
     5041 cgtaccgtgt aacggaccgg cgctgtcgct gccgttatat ccgtgcgata acgtcgattt
     5101 ccaggtcacc acctactaca gcaacatttg cccaaatggt gcttatcagg gttatggcgc
     5161 accgaaaggt aacttcgcta tcaccatggc attagcggaa ctggctgaac agttacagat
     5221 cgaccaactg gaaattatcg aacgtaaccg ggtacacgaa gggcaagagc tgaaaattct
     5281 cggtgcaatc ggtgaaggta aagcgccgac ctccgttcct tccgccgcca gctgtgcact
     5341 ggaagagatc ctgcgtcagg gtcgcgagat gatccaatgg tcgtcaccga aaccgcaaaa
     5401 tggtgactgg cacatcggtc gcggcgtcgc cattatcatg cagaaatcgg gtatcccgga
     5461 tatcgatcag gctaactgca tgatcaaact ggaatcggac ggcaccttta tcgttcattc
     5521 tggcggtgcg gatattggta ctggtctgga tactgtagtg acgaaactgg cagcagaagt
     5581 gctgcactgc ccaccgcagg acgtgcatgt tatctccggt gataccgatc atgcgttgtt
     5641 tgataaaggc gcatatgcct cgtccggtac ttgcttctcg ggtaacgcgg cgcgtttggc
     5701 agcggaaaat ctacgggaga aaattctgtt ccacggcgcg caaatgttgg gtgaggcagt
     5761 ggcagatgtt caactggcaa cgccgggcgt cgtgcgcggc aagaaaggcg aagttagttt
     5821 cggggatatt gcccataaag gcgaaaccgg catcggcttt ggttcactgg tgggaactgg
     5881 cagttatatc acgcctgatt tcgccttccc gtatggcgca aacttcgctg aagttgccgt
     5941 caacacgcgt acgggtgaaa tccgcctgga taaattctac gccttgctgg actgcggtac
     6001 accggtcaac ccggaattag cattgggaca aatctacggt gctaccctgc gagctatcgg
     6061 ccacagtatg agcgaagaga tcatttatga cgccgaaggt cacccgttaa cgcgtgattt
     6121 acgcagttac ggcgcaccga aaattggtga cattccgcgt gatttccgcg ctgtgctggt
     6181 gccgagcgac gataaagtcg gcccgttcgg ggcgaaatcg atctcggaaa tcggtgtaaa
     6241 tggcgcagct ccggcgattg ctaccgcaat tcacgataca tgcggcatct gggcagaaga
     6301 aagtcaaagt gggcatggga acggatatcg gtgccggaac aactttcaac atgctgcaaa
     6361 cgctgaacga agcctacaaa gtgttgcaat tacaaggcta tcgcctctcg gcatatgaag
     6421 cgttttacct ggccacgctc ggcggagcga aatctctggg ccttgacgat ttgattggca
     6481 actttttacc tggcaaagag gctgatttcg tggtgatgga acccaccgcc actccgctac
     6541 agcagctgcg ctatgacaac tctgtttctt tagtcgacaa attgttcgtg atgatgacgt
     6601 tgggcgatga ccgttcgatc taccgcacct acgttgatgg tcgtctggtg tacgaacgca
     6661 actaataata aaactttaac atcctcgtga ggacatcatt atgtctggag acatcctaca
     6721 aacaccggac gcaccaaagc cacagggcgc gctggataat tattttaaaa ttaccgctcg
     6781 tggcagtacc gttcgtcagg aagtactggc tggcttaacg acctttctgg ccatggttta
     6841 ttccgttatc gtcgttccgg gaatgctggg caaagcagtt tttcctcccg cagctgtgtt
     6901 tgttgccacc tgtctggtcg cgggcttcgg ctcgttgctg atggggttgt aggccaattt
     6961 gccaatggcg attggttgtg cgatttcctt gacggcattt accgcattca gtctggtact
     7021 cgggcaacaa attagcgttc ctgtcgcact ggtcgcattt tacgcgggtg gttgggcaat
     7081 ctaatagatt ctccgcgcct ttcagcgcgg agatttcttt cacagaggat tctcaccgct
     7141 ggcaacaata cgctgctggc ggactcgcct gagttctctt tcagtaagca actgcagcgc
     7201 ctgtgtcggg caagattcaa cacaggctgg cccctcttcc cgctgttcac agagatcgca
     7261 tttcacaatt tgttgtcggg tatcccccgg caacgactga atggtgatca ttccaaacgg
     7321 gcacgcactg acgcaactct gacagccaat acaacgggca gaattggtct gcaccacctg
     7381 ctcgcccatc gtcagcgccc ccacggggca agcgccaaca caaggggcgt tttcacactg
     7441 atggcacatc accggcgcac tgatgctgtc cagtcgctgt accttcagcc ggggtaaaaa
     7501 gacatcggca ttcaactcct gttctgaagg gtgagcgacc acacaggcta cttcacaagt
     7561 acggcagcca atacagtcag ccggattaac gataattaac gatttcatca cgatgccttc
     7621 gtatcaaaca gagttaacat atcgcgcgcc gcctgccttc ctgcggccat tgcagtgaca
     7681 accagatccg cgccatgaac tgcatcacca ccagcaaaga cttttttcag atgcgtctgg
     7741 gtaggtaaat acccgacgtc accggtttga atcaggcccc atttatcgag tttaattccg
     7801 ctgccctgca accacggcat ggcatgcgcc tggaaaccaa aggccataat cagaacatcg
     7861 gcgggcaatt caaactctga acccgctacc ggacgaggac gacggcgacc atccggcccc
     7921 ggctcaccca tggcggtacg aatcaggccc accgcagtta aacgcccatc ttcgtcacaa
     7981 gcgatatatt gcggttgaac attgaactga aactcgacac cttcctcgcg cgcattaacc
     8041 acctctttgc gcgagcccgg catactgact tcatcacgac gatacgcgca ggtcacgctg
     8101 gcagcattga ggcgaatgga agtccgcaaa caatccattg tcgtatcgcc accgcccaat
     8161 accacgactc gcttaccttc cacgtccgtc agcggatact cttcagactc cggcaatccc
     8221 atgagctggc gggtatgggc agtcaggaac ggtagagcct gaatgacacc gggcgcatct
     8281 tcatgcggca gatctgctcg catcatcccg taagtcccca cgccgatgaa aactgcatca
     8341 tattcagaag ttaaatcgct aaaggtaata tcgcggccaa tttcacagtt aagatgaaag
     8401 tcgattccca ttgcggtgaa tatctctcgc cgctggctta ataccgtttt atcgagtttg
     8461 aaaggaggaa tgccaaaagt cagcataccg ccaatttctg gatggcgatc aaagacatca
     8521 acctgaactc ccgcgcgcgc cagaatatca gcacacccta accctgctgg cccagcgcca
     8581 atcaccgcca ctttttcgat acggggaaca actttgctga catcgggacg ccagcccatc
     8641 gccagcgcgg tatcggtgat gtagcgttcc agattaccga tagagactgc gccagagtga
     8701 tctttcaaag tacatgcgcc ttcacaaaga cggtcctgtg ggcataccct gccgcagatt
     8761 tctggtaagg aactggtctg gtggcaaagt tctgccgctt caataatctt tccttcctgt
     8821 accagacgga tgtaatccgg aatagcgtta tgcagcgggc aatgccagtt gcagttagct
     8881 ttttcggcac aataaacaca gcggtcactc tcataagtcg cttgcagtgg atccagcccg
     8941 caatagattt cgccaaagtg ggttttccgt tcactcgctg aaattttatc tgcgccttta
     9001 cgcgagttaa cggggagcaa cgctgcactg cgagatggct gagcgtctga tgacgctttt
     9061 cctgctgccg ttttgcgctg gcgagccacc tttatccact gtaacccttt atcgtccatc
     9121 agtcgtaacg cctgtgttgg gcagacttca atacaggctt gcgtgccgga actgcgctgg
     9181 ttacaaaggt cgcatttctg cgcaatcgta tcgaccatct caacgacgcc aaaggggcaa
     9241 gcgattgcgc atcttttaca gccaatacat ttttgctcgt ccagttgtac gctatcggac
     9301 tggaaagtca gagcattaac cggacaagcc gtaacacaag gggcattgtt gcagtgatgg
     9361 caggccaccg gattcgcagc ctggcctttc cctacaacgt ggatacgcgg tcgaaagtca
     9421 ctgtgactca gcggccagtt ttcttgattg tgtgccaccg cacaggcaat ttcacaagca
     9481 tgacagccta tacattccgc agcttcagca gcgataaact tattcatttg catccctttc
     9541 at
//
LOCUS       DAEEKQ010000148         9456 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.150, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000148 DAEEKQ010000000
VERSION     DAEEKQ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9456
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.150"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..558
                     /locus_tag="KLK57_003345"
     CDS             <1..558
                     /locus_tag="KLK57_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBE0893633.1"
                     /translation="KIVLQWDEMIRTAGSLKLGKVQASVLVRSLLKSERPSGLTQAII
                     EVGRINKTLYLLNYIDDEDYRRRILTQLNRGESRHAVARAICHGQKGEIRKRYTDGQE
                     DQLGALGLVTNAVVLWNTIYMQAALDHLRAQGETLNDEDIARLSPLCHGHINMLGHYS
                     FTLAELVTKGHLRPLKEASEAENVA"
     gene            complement(594..1009)
                     /locus_tag="KLK57_003346"
                     /pseudo
     CDS             complement(594..1009)
                     /locus_tag="KLK57_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(1258..1659)
                     /gene="pemK"
                     /locus_tag="KLK57_003347"
     CDS             complement(1258..1659)
                     /gene="pemK"
                     /locus_tag="KLK57_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin
                     endoribonuclease PemK"
                     /protein_id="HBE0893634.1"
                     /translation="MLKYQLKNENGWMHRRLVRRKSDMERGEIWLVSLDPTAGHEQQG
                     TRPVLIVTPAAFNRVTRLPVVVPVTSGGNFARTAGFAVSLDGVGIRTTGVVRCDQPRT
                     IDMKARGGKRLERVPETIMNEVLGRLSTILT"
     gene            complement(1592..1849)
                     /gene="pemI"
                     /locus_tag="KLK57_003348"
     CDS             complement(1592..1849)
                     /gene="pemI"
                     /locus_tag="KLK57_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000557619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PemI"
                     /protein_id="HBE0893635.1"
                     /translation="MHTTRLKRVGGSVMLTVPPALLNALSLGTDNEVGMVIDNGRLIV
                     EPYRRPQYSLAELLAQCDPNAEISAEEREWLDAPATGQEEI"
     gene            complement(1942..2595)
                     /locus_tag="KLK57_003349"
     CDS             complement(1942..2595)
                     /locus_tag="KLK57_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HBE0893636.1"
                     /translation="MIQTRNQYLQFMLVMLAAWGISWGARFVMEQAVLLYGSGKNYLF
                     FSHGTVLMYLLCVFLVYRRWIAPLPVVGQLRNVGVPWLVGAMAVVYVGVFLLGKALAL
                     PAEPFMTKLFADKSIPDVILTLLTIFILAPLNEETLFRGIMLNVFRSRYCWTMWLGAL
                     ITSLLFVAAHSQYQNLLTLAELFLVGLITSVARIRSGGLLLPVLLHMEATTLGLLFG"
     gene            complement(3534..4391)
                     /gene="repA"
                     /locus_tag="KLK57_003350"
     CDS             complement(3534..4391)
                     /gene="repA"
                     /locus_tag="KLK57_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HBE0893637.1"
                     /translation="MTDLHQTYYRQVKNPNPVFTPREGAGTLKFCEKLMEKAVGFTSR
                     FDFAIHVAHARSRGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLSELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVVWENKQRKKQGLDTLGMDELIAKAWRFVRERFRSYQTELKSRGIK
                     RARARRDADRERQDIVTLVKRQLTREIAEGRFTANREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            complement(4384..4458)
                     /gene="tap"
                     /locus_tag="KLK57_003351"
     CDS             complement(4384..4458)
                     /gene="tap"
                     /locus_tag="KLK57_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HBE0893638.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            complement(4696..4950)
                     /gene="repA"
                     /locus_tag="KLK57_003352"
     CDS             complement(4696..4950)
                     /gene="repA"
                     /locus_tag="KLK57_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HBE0893639.1"
                     /translation="MSQTENAVTSSSGAKRAYRKGNPLSDAEKQRLSVARKRASFKEV
                     KVFLEPKYKAMLMQMCHEDGLTQAEVLTALIKSEAQKRCM"
     gene            complement(5190..5780)
                     /locus_tag="KLK57_003353"
     CDS             complement(5190..5780)
                     /locus_tag="KLK57_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBE0893640.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSGGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVERRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            complement(5818..6027)
                     /gene="hha"
                     /locus_tag="KLK57_003354"
     CDS             complement(5818..6027)
                     /gene="hha"
                     /locus_tag="KLK57_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HBE0893641.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLLNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            complement(6073..6534)
                     /locus_tag="KLK57_003355"
     CDS             complement(6073..6534)
                     /locus_tag="KLK57_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HBE0893642.1"
                     /translation="MRKYIPLVLFIFSWPVLCADIHGRVVRVLDGDTIEVMDSRKAVR
                     IRLINIDAPEKKQDYGRWSTDMMKSLVAGKTVTVTYFQRDRYGRILGQVYAPDGMNVN
                     QFMVRAGAAWVYEQYNTDPVLPVLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            complement(6779..6991)
                     /locus_tag="KLK57_003356"
     CDS             complement(6779..6991)
                     /locus_tag="KLK57_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001369432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBE0893643.1"
                     /translation="MNGTGRDSQNSGVWRYRQVGRRAVRLEVRGDWPGAAEAWLRAAQ
                     SAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            complement(7120..7680)
                     /locus_tag="KLK57_003357"
     CDS             complement(7120..7680)
                     /locus_tag="KLK57_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBE0893644.1"
                     /translation="MTEQKRPVLTLKRKTEGETPTRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELTAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEV
                     YAAERLDKIRRQNRIKAELQAVLDEQ"
     gene            complement(7783..8643)
                     /locus_tag="KLK57_003358"
     CDS             complement(7783..8643)
                     /locus_tag="KLK57_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0893645.1"
                     /translation="MKITDHKLSEGIALTFRVPEGNIKHPLIILCHGFCGIRNVLLPC
                     FANAFTEAGFATITFDYRGFGESDGERGRLVPAMQTEDIISVINWAEKQECIDNQRIG
                     LWGTSLGGGHVFSAAAQDQRVKCIVSQLAFADGDVLVTGEMNESERASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLIVIAGQDSVNPPEQGKALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            complement(8702..9448)
                     /gene="traX"
                     /locus_tag="KLK57_003359"
     CDS             complement(8702..9448)
                     /gene="traX"
                     /locus_tag="KLK57_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HBE0893646.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSYRLYRAEDRAERLALLACLLAVIPALNLASSDAAAVAGLVMTVLTVGLVSC
                     AGKSLPRFWPGDFFPVFYACHLAVLGVLAL"
BASE COUNT         2340 a         2549 c         2197 g         2370 t
ORIGIN      
        1 aaaatagtcc ttcagtggga cgaaatgatc cggaccgctg gctccctgaa gctgggcaaa
       61 gtacaggctt cagtgctggt ccgttcattg ctgaaaagtg aacgtccttc cggactgact
      121 caggcaatca ttgaagtggg gcgcatcaac aaaacgctgt atctgcttaa ttatattgat
      181 gatgaagatt accgccggcg cattctgacc cagcttaatc ggggagaaag tcgccatgcc
      241 gttgccagag ccatctgtca cggtcaaaaa ggtgagataa gaaaacgata taccgacggt
      301 caggaagatc aactgggcgc actggggctg gtcactaacg ccgtcgtgtt atggaacact
      361 atttatatgc aggcagccct ggatcatctc cgggcgcagg gtgaaacact gaatgatgaa
      421 gatatcgcac gcctctcccc gctttgccac ggacatatca atatgctcgg ccattattcc
      481 ttcacgctgg cagaactggt gaccaaagga catctgagac cattaaaaga ggcgtcagag
      541 gcagaaaacg ttgcttaacg tgagttttcg ttccactgag cgtcagaccc caatacgtgc
      601 gcaacaaccg tcttccggag cctgtcatac gcgtaaaaca gccagcgctg gcgcgattta
      661 gccccgacgt atccccactg ttcgtccatt tccgcgcaga cgatgacgtc actgcccggc
      721 tgtatgcgcg aggttaccga ctgcggcctg agttttttaa atggcggaaa atcgtgttga
      781 ggccaacgcc cataatgcgg gcggttgccc ggcatccaac gccattcatg gccatatcaa
      841 tgattttctg gtgcgtaccg ggttgagaag cggtgtaagt gaactgcagt tgccatgttt
      901 tacggcagtg agagcagaga tagcgctgat gtccggcggt gcttttgccg ttacgcacca
      961 ccccgtcagt agctgaacag gagggacagc tgatagaaac agaagccact ggagcacctc
     1021 aaaaacacca tcatacacta aatcagtaag ttggcagcat caccggaact tcaaaactgc
     1081 gcaaaacacg caaatacgca caagaaagta gggctgcgct tggaattcgg aaaataaacc
     1141 acatcaaaac tactttttag ccaaataacg tttgggaatc accagaatgg tgggacaaca
     1201 gcggttttag tgccctaaat cgtacgtttt catccagttg cccctcaaac cccatgttca
     1261 agtcagaata gtggacaggc ggccaagaac ttcgttcatg atagtctccg gaacccgttc
     1321 gagtcgtttt ccgccccgtg ctttcatatc aattgtccgg ggttgatcgc aacgtacaac
     1381 acctgtggta cgtatgccaa caccatccaa cgacaccgca aagccggcag tgcgggcaaa
     1441 attgcctccg ctggttacgg gcacaacaac aggcaggcgg gtcacgcgat taaaggccgc
     1501 cggtgtgaca atcagcaccg gccgcgttcc ctgctgctca tgacctgcgg taggatcaag
     1561 cgagacaagc cagatttccc ctctttccat gtcagatttc ctcctgacca gtcgccggtg
     1621 catccagcca ttctcgttct tcagctgata tttcagcatt cggatcacac tgtgccagta
     1681 gctcagccag tgaatattgc gggcgtctgt acggctcaac aatcagccgg ccattatcaa
     1741 tgaccatgcc aacttcatta tctgtgccca gagacagcgc attcagcagt gccggtggga
     1801 cggtcagcat aactgagccg ccaaccctct tcagtcgggt ggtatgcatt cttcacctcc
     1861 ataaaagtta tatttaaata taacatccac taaaaaaaca caccaggctt taacgcacaa
     1921 tgtttaataa aaatataact ttcaaccaaa cagtaaaccc agcgtggttg cttccatatg
     1981 cagcaatacc ggcagcagca ggccaccact tctgatcctg gccactgatg taatcaaccc
     2041 caccaggaac agttctgcca gtgtcagcag gttctgatac tggctgtgcg cggcgacgaa
     2101 caacaacgac gttatcagcg cccccagcca catcgtccag cagtaccgtg aacggaagac
     2161 gttcagcata atcccccgga acagcgtttc ctcattcaac ggggcaagga taaagatggt
     2221 cagcaacgtc aggatcacgt caggtatgga cttatcggca aaaagtttcg tcataaatgg
     2281 ctcagcaggc agagccagcg ccttaccgag cagaaataca ccgacataca ccacggccat
     2341 cgcaccgacc agccacggta cgccaacgtt gcgcagctga ccaacgaccg gtagcggagc
     2401 tatccaacgg cggtatacca ggaaaacaca cagcaggtac atcagaacag taccatgact
     2461 gaagaacaaa tagttttttc ctgatccata aagcagaacg gcctgctcca tgacaaatct
     2521 ggctccccaa ctaatgcccc atgcagccag cataaccagc ataaactgca gatattgatt
     2581 acgtgtttga atcattgcat cgcctgtaaa tttttaactt gtcctatttt tgtcattacc
     2641 acgtatatac acatgtataa caattcagat atcgttacca ggatatgccg catcagcggc
     2701 atggaaggcg gcactctgtt gtttcatatg atacaggagt aaaaccgccg aagcccggcg
     2761 taagccggta ctgattgata gatttcacct tacccatccc cagccctgcc agaccatacc
     2821 cgctttcagc catgagagag cttctgtgcg cggtcggagt ggtcccgacg agggtttacc
     2881 cgaagtcggg gcgtatctcc gcgttagcgg gccgtgaggg ccgcttacga gcgtgtactg
     2941 agaacttcca gcgagaagac tgacagcgat gaagatgtag ttacaacatt cataattaaa
     3001 agcgactctg ttccggccct ttgggccggg gcggggccgc ttttcagtta tgagggaggg
     3061 gctttgtggt ttcggttctg cgctggaccg gggtttttct ggaggttgtt tttgtgtgtt
     3121 gtaactaaag tggctccggt cggggcccgc cgcttgcggt gggaggtgca tatctgtctg
     3181 tccacaggac aggcagtgaa taggttttct ttttaaatga atgtaattaa gtagtttaaa
     3241 ggagatataa acaggtgttt aaaagataca ttgcaccctg taagactggc ggctggcgct
     3301 ttatgacatg aacggttgta accttatggg gaagtccctt gcagttaaat gtggataagc
     3361 aaaattcccc gtctgtgagg cgtgttttgt atcgaaaaca gggggggccg gatgcaccag
     3421 aagctggatg atgaggttgt tttttgtatg tggtgctgat tttttgtgcg ctgtcgggct
     3481 tcagacgtgc ggaagcctcc ggcgcaggcc ggattattca gaggaggtca ctttcagggg
     3541 gaagctgtgg ccagccggct gtaattacgg ttacgtgaca gaatcatgcg ctccttcaca
     3601 cgacgctcaa cttcgcgttt taccgcctca cgattggcag tgaagcgccc ttccgcgatt
     3661 tcgcgcgtca gctgccgttt caccagggtg acaatatcct gacgttccct gtccgcatca
     3721 cgacgcgcac gggcacgctt tattccccgg gacttaagct ctgtctgata actgcgaaaa
     3781 cgctcacgaa caaaacgcca ggctttcgct atcagttcat ccatgcccag ggtatccagc
     3841 ccctgctttt tgcgttgttt gttttcccat accacacggc tgcggcgcgc ggcggccact
     3901 gcctcctctg atacatcgag ggcagcaaac agtgcagatg tgaacgtgat atcggtcgga
     3961 atgtagcacc cgataagcgg gtcatattcc gtctggtagg taatcagtcc cagctctgac
     4021 aggaacgtca gggcccgggt ggcacgggtg atggagagtt ttccggcagc agactccgtc
     4081 gccagtccgc actcaatggc cagcgtggtg atggagcact ggacgcggtt ggccagcggg
     4141 tcatagtgga aacacagccc ctgcaggagc gcatcaatag cccgtcgacg cagcactggt
     4201 ggcatgcgtc gacgcagacc acgcgaacgg gcatgcgcca catgaatggc gaaatcaaaa
     4261 cgggaagtga agcccaccgc cttttccatc agtttttcgc agaacttcag cgttcctgca
     4321 ccttcacggg gtgtaaacac cggattcggg ttctttacct ggcggtaata cgtttggtga
     4381 agatcagtca caccatcctg cacttacaat gcgcagaagg agcgagcaca gaaagaagtc
     4441 ttgaactttt ccgggcatat aactatactc cccgcatagc tgaattgttg gctatacggt
     4501 ttaagtgggc cccggtaatc ttttcgtact cgccaaagtt gaagaagatt atcggggttt
     4561 ttgcttttct ggctcctgta aatccacatc agaaccagtt ccttgccacc ttacggcgtg
     4621 gccagccaca aaattcctta aacgatcagt aatctagcac taatcttctg aacactcaag
     4681 aatgtaagcc catcatcaca tgcatcgttt ttgcgcttca ctttttatca gtgcggtcag
     4741 aacttcagcc tgagtcagac catcttcatg acacatttgc atgagcatgg ccttatactt
     4801 tggttcaaga aatactttta cttccttgaa cgaagctctt ttacgggcca ctgataatct
     4861 ttgtttctct gcatcagaaa gcggattccc ctttctgtat gctcgttttg cgccagatga
     4921 ggaagtcact gcattttctg tctgcgacat ctcgcctcct caatactcaa acagggatcg
     4981 tttcgcagag gatactacag ttttttgaaa tcagcgactt gagaattgtg acgaagatct
     5041 gtgttggcgc acaaatcaac ggggattact gtcgtttaat gtgatttaaa ctgtgaaata
     5101 gtatggtttt cagttattga aacgccggga gcggggaaaa cttgcttttt cccgtttccg
     5161 gggttggaca actgagcaac gcgaaggcgt cagctacgat gttccgggga ctgctgatca
     5221 gcccctgttg tattcagcca ttctccggtc atctgcagca gttttctggc atcgtggctt
     5281 ctgagcaacg gaataccagc ctgcctcaga acctcttcca gaagaatatc ccggcgtctg
     5341 cgttccggtc gtaaatggct ggcatcatcc agctctactg ctgcaacaat actgaacgaa
     5401 cgccgctcaa cgataaccac atcaacatgc cactgagaca ccatcctgaa taactgccac
     5461 cactggcgtg atcgtggccg gatattcccg ttcagctgga cgatatccgc gacccgtacc
     5521 tgcggacaca gataccaccg cttcatatcc acaattctga acagtccttt cataaaactg
     5581 acttcccttt ctgtcagcag cgtcttctga cgttgccagt attcaccgcc ggacagtatt
     5641 cgctctgcat caccggtccg tatacctgcc gcatgcaatt gcatcagcaa aggagaatgc
     5701 tgatttacac ttctgaggat aatccggata agtaacgctg caataatcag aacaataaga
     5761 ataaaaataa tcagcttcat tgtttatctg ctcctggtaa ttaattatgc tgaattatta
     5821 ccttacatat ttccatatct cttttggtat cctgtcatat aactttccgg tgattaattc
     5881 cgctaaccgg tggtctgctg ctgaattaaa tgattcacgt tcactgttta ataaactgtc
     5941 tctgttttta tgaataattt tttccagcgt attgactgat gaacatcgcc gtaactgata
     6001 cagccattcc tgttttgttt tcgccatttt tattcccgct gagaacagat gaatgataag
     6061 attgccatcg ctttatttac ggtgtctcca tatccagggg ggaaccggat cagcatctga
     6121 ccagagccca cgtttttgtt gtctggcttc gttctgaagg acaggcagca cgggatctgt
     6181 gttgtactgc tcatataccc aggcagcccc ggcccggacc ataaactgat taacattcat
     6241 tccgtctggt gcataaacct gcccgagaat acgaccataa cgatctcgct gaaaatatgt
     6301 gacggtaacc gtttttcctg caaccagtga tttcatcatg tccgttgacc accggccata
     6361 atcctgtttc ttttccggtg catcaatatt tatcagcctg atccgcactg ccttccgtga
     6421 gtccatgacc tcgatggtat caccgtccag tacacgaaca acccgtccat gaatatctgc
     6481 acacaatacc ggccatgaaa agataaataa taccagtgga atatatttcc tcatgttatt
     6541 ctccatatca gataaatggc acctgtattt caggtaatcc tgaatatcgc agcacacact
     6601 gaataacacc ttattttgtg aaattaatca caaaatataa aatcacgact taatacggaa
     6661 aaaactctgt gctactcaga actgtccggt catacttccc gaccggatat taaaaaaagc
     6721 aaatctcatg cattattcag ccttctgaat ccctggagta atgaaggctg ctttttcgtt
     6781 acacccttcc ccggcagcgc cggtggcagt gttcagccct ttttcgggca aactgctgcc
     6841 agtctgtccg gggagcagac tgcgcagccc gtagccatgc ctcagcggct cccggccagt
     6901 cccccctgac ttccagccgg acagcccggc gtccgacctg ccggtagcgc cataccccgg
     6961 agttttgcga atcacgccct gttccgttca ttttccttcc ccctttaatc caggccggtg
     7021 aatgtgttat aaatttttat aaccttcttc tgccgggtat ggaagaaggt ttcagagaag
     7081 ccttattttt ccgcctcctt tagtgtgaag gaggcttttt tattgttcat caagcacggc
     7141 ctgaagttct gcctttatcc ggttctggcg gcggatttta tccagacgct ctgccgcata
     7201 cacttcttcc tcctgagaaa tatgctccgt cacataccct tccgtgtcat accggcaggc
     7261 accggctttc atggcacaca gatagctttc tgaacgggtg atggccttca gcgccctgcg
     7321 cagtttttta tgcgagagcg ggatattccg ctgcgccacg tcttccagta acacgtcccg
     7381 gataccgcag gccagaagcc ggggtgtgtc accgtcaaat aatcccggcc accagggttt
     7441 cagggtgttc acggcttcat ccagtgtggg caggttcaga taaatggaca gcgcctgtct
     7501 ggcctgcgct ttttttgctg tcagctctgc ttcccgggca gccttctccg ccagtttctg
     7561 ctttttcacc ttccattttg gtggcgtggt gacattgatg atggttttcc ggctccgggt
     7621 cggtgtttcc ccttccgttt tccgtttcag tgtcagtacc ggtcgtttct gctctgtcat
     7681 gattgccctg acctttgtga aaatatgtcc tccaggtcgt tgaaactact cactatgata
     7741 ttaagctgac ctcaacgacc tctgacatat taataccgac aattaaagat gttttttaaa
     7801 ccattgcgtc tgtactgccg ccacacgttc aaaaaacgcg ccttcataaa tatcataatg
     7861 gcatgcgtca gcttcctcgt ataattcctt agtaccagaa gcaacagcat catataaggc
     7921 ttttccctgt tctggtggat taacactatc ctggccggca ataacaatca aaactgggca
     7981 ctgtactttg gctgcgctct ctgccggttt atattgcaac gtctccataa ccgtaaggaa
     8041 aggtattttt atatccattt caggatactg accttttacc ttctcgaaga atacctttga
     8101 ttcattatca ctcagtaccc gggtaacccc cacaaacatc tctttcccgg tgtttttctt
     8161 cttctctgcc attttattca gcgtggacag aaaagacgct ctctccgatt cattcatctc
     8221 tccggtaacc agaacatcac cgtcagcaaa agccagctgg ctgacaatac attttacgcg
     8281 ttggtcctgc gcagctgcac tgaagacatg cccccctccc agcgatgttc cccataaccc
     8341 aatacgctga ttatcaatac attcctgctt ttctgcccaa ttgataactg aaataatatc
     8401 ttcggtctgc attgcaggaa ccagacgccc tcgttctcca tcactctcac cgaatcctcg
     8461 ataatcaaaa gtaatcgttg cgaatcctgc ttctgtgaag gcattcgcaa agcaagggag
     8521 cagaacattc ctgataccgc aaaacccatg gcataagata atcaaaggat gttttatatt
     8581 tccttcagga acacgaaatg tcagggcaat accttcagag agtttgtgat ccgtaatttt
     8641 cataaatatc ctgtgttatg ttgtttatta ataatcagaa tcagcgtcag tatcatacca
     8701 gtcacaacgc cagaacgccc agcacagcca gatgacaggc gtagaatacc gggaaaaaat
     8761 ccccgggcca gaaacggggt aatgattttc ctgcacacga caccagacca acggtcagca
     8821 ccgtcattac aagaccggcc accgccgctg catcactgga ggcaaggtta agtgccggaa
     8881 taacggccag caggcatgca agcagcgcca gacgttccgc tctgtcttcc gcccggtaca
     8941 gtcggtaact gaccgccagc atcagcagcc cggcaatgcc gtaactggtg ccggacaaag
     9001 gcccccacag ggccatcaga agaatggccg ctgctgtacg ccacccactg cgcgtttcac
     9061 accacgtcag cacctgtgcc gccactgcaa aggcaaacag gatattcccc tcataccagg
     9121 gaaagccggc cagataatat gcgaactggg caataattcc ccatccccac agccggttaa
     9181 tggccggttg ccggatatgc gcatgacggg acagattcag cccccacacc agggcaaaca
     9241 gcggaaaggc tcctcgcccg gccagaaaca tccattcctg cttcagctgg aatatcagat
     9301 tgatgtggtc cagtaccatc agcaccagcg ccaccgtttt gatgatatcc cgctgtccgg
     9361 gtgaccagac cagaaacgac tgcaaccagg tatcggtccg tgcagccagt gaatcgttac
     9421 gtgtcgtgtt cgtgttatcc gttgtcatca gcgttt
//
LOCUS       DAEEKQ010000149         9432 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.151, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000149 DAEEKQ010000000
VERSION     DAEEKQ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9432)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9432)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9432
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.151"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            205..1071
                     /gene="folD"
                     /locus_tag="KLK57_003360"
     CDS             205..1071
                     /gene="folD"
                     /locus_tag="KLK57_003360"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HBE0893647.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            1073..1285
                     /gene="ybcJ"
                     /locus_tag="KLK57_003361"
     CDS             1073..1285
                     /gene="ybcJ"
                     /locus_tag="KLK57_003361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HBE0893648.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKADGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            1393..1914
                     /locus_tag="KLK57_003362"
                     /pseudo
     CDS             1393..1914
                     /locus_tag="KLK57_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
     gene            complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="KLK57_003363"
     CDS             complement(1950..3335)
                     /gene="cysS"
                     /locus_tag="KLK57_003363"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HBE0893649.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYAADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTEKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            3509..4003
                     /gene="ppiB"
                     /locus_tag="KLK57_003364"
     CDS             3509..4003
                     /gene="ppiB"
                     /locus_tag="KLK57_003364"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HBE0893650.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVEGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            4006..4728
                     /gene="lpxH"
                     /locus_tag="KLK57_003365"
     CDS             4006..4728
                     /gene="lpxH"
                     /locus_tag="KLK57_003365"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HBE0893651.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDEPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            4846..5355
                     /gene="purE"
                     /locus_tag="KLK57_003366"
     CDS             4846..5355
                     /gene="purE"
                     /locus_tag="KLK57_003366"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HBE0893652.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVLHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGTA"
     gene            5352..6419
                     /gene="purK"
                     /locus_tag="KLK57_003367"
     CDS             5352..6419
                     /gene="purK"
                     /locus_tag="KLK57_003367"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HBE0893653.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYTLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(6556..7449)
                     /locus_tag="KLK57_003368"
     CDS             complement(6556..7449)
                     /locus_tag="KLK57_003368"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HBE0893654.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(7446..8099)
                     /locus_tag="KLK57_003369"
     CDS             complement(7446..8099)
                     /locus_tag="KLK57_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBE0893655.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAAGDPLPAELRQFFAYSDNLQLVTTAVSVSYLR
                     YAAQGYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(8110..9369)
                     /locus_tag="KLK57_003370"
     CDS             complement(8110..9369)
                     /locus_tag="KLK57_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBE0893656.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNEGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAILEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMERGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRRVVETDITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
BASE COUNT         2102 a         2673 c         2498 g         2159 t
ORIGIN      
        1 tagacaagga aataagtcag ttccagattt acaacgccat catggacgaa aaatgaagct
       61 ttcaatctca gcgacggtgc gccccacctt cgcaagaggt cgcttcacgc gataaatctg
      121 aaacgaaacc tgacagcgcg ccccgcttct gacaaaatag gcgcatcccc ttcgacctac
      181 gtaacagatg gaatcctctc tctgatggca gcaaagatta ttgacggtaa aacgattgcg
      241 cagcaggtgc gctctgaagt tgctcaaaaa gttcaggcgc gtattgcagc cggactgcgg
      301 gcaccaggac tggccgttgt gctggtgggt agtaaccctg catcgcaaat ttatgtcgca
      361 agcaaacgca aggcttgtga agaagtcggg ttcgtctccc gctcttatga cctcccggaa
      421 accaccagcg aagcggagct gttggagctt atcgatgcgc tgaatgccga caacaccatc
      481 gatggcattc tggttcaact gccgttaccg gcgggtattg ataacgtcaa agtgctggaa
      541 cgtattcacc cggacaaaga cgtagacggt ttccatcctt ataacgtcgg tcgcctgtgc
      601 cagcgcgcgc cgcgtctgcg cccctgcacc cctcgcggca tcgtcacgct gcttgagcgt
      661 tacaacattg ataccttcgg cctcaacgcc gtggtgattg gcgcatcgaa tatcgtcggt
      721 cgtccaatga gcatggaact gttgctcgca ggttgcacca ctaccgtgac tcaccgcttc
      781 actaaaaatc tgcgtcacca cgtagaaaat gccgatctac tgatcgttgc cgtcggcaag
      841 ccgggcttta ttcctggtga ctggatcaaa gaaggcgcaa ttgtgattga tgtcggcatc
      901 aaccgtctgg aaaatggcaa agttgtgggc gacgtcgtgt ttgaagacgc ggctaaacgc
      961 gcctcataca ttacgcctgt tccgggcggc gttggcccga tgacggttgc cacgctgatt
     1021 gaaaacacgc tacaggcgtg cgttgaatat catgatccac agggtgagta acatggcgac
     1081 attttcttta ggtaaacatc cgcacgttga gctgtgcgac ttgctgaaac tggaaggctg
     1141 gagcgaaagc ggcgcgcagg cgaaaatcgc gattgccgaa ggccaggtga aagccgacgg
     1201 cgcggttgaa acgcgcaaac gctgcaaaat cgtcgccggt cagacagtga gttttgcagg
     1261 tcacagcgta caggttgttg cctgatccct ttcccggcct gtatcaacag gccggttctc
     1321 ttttcagact tttgccctct acgccccgtt catccatgct gatagcatcg acattttgcc
     1381 gaagcgacca tcatgccaac cgttattacg cacgccgccg ttcccctatg cattggttta
     1441 gggttgagtt cgaaagtcat ccctccgcgt ctgttatttg cctgaatcat cctggcgatg
     1501 ctgcccgacg ccgacgtatt gtcgtttaaa tttggcgttg cttacggcaa tgtttttggt
     1561 catcgcgggt ttacccattc gctggtgttt gcgtttgttg tcccgctgtt atgtgtgctg
     1621 attggacgcc gatggttcag ggctgggctg attcgctgct ggctgttttt aaccgtctcg
     1681 ttgctatcgc acagcatgct ggattcggta accactggcg gtaagggtgt tggctggctg
     1741 tggccgtggt cagatgaacg ctttttcgct ccctggcagg tgattaaagt cgcgccgttt
     1801 gcattgtctt gttacaccac gccgtacggg catcaggtga ttatttctga attgatgtgg
     1861 gtgtggttgc cggggatgct actgatggga atgttgtggt ggcgcagacg ataaccggat
     1921 gcgaaaactc gcatccggca atagcgcaat tacttacgac gccaggtggt cccttgcggg
     1981 ccatcttcca gaacgatccc catctcgtta agacggtcac gcgccgcatc tgccgccgcc
     2041 cagtctttcg ctttacgggc atccagacgc tgttgaatta acgcttcaat ctcagccact
     2101 tcgctgtcgt ctgcctgcgc gccgctttgc aggaacgctt ccggttcttg ctccagcagg
     2161 cctaatacag cggaaagttt acgcaggtga gatgccattg cattcgccgc tgccatatct
     2221 tctgctttca gacggtttac ttcacgcgcc atatcaaaca gtacggaata ggcttccggg
     2281 gtgttgaaat cgtcgtccat cgcttcaata aagcgcgctt caaacgcttc gccaccggca
     2341 ggagcaacgg ttttttctgt gccgcgcagg gcagtgtaga gacgctccag cgccgcacgc
     2401 gcctgcttca ggttctcttc gctgtagttc agctggctgc gatagtggcc cgacatcagg
     2461 aagtaacgca cggtttcagc atcgtagtat ttcagcacat cgcgcacggt aaagaagtta
     2521 cccagcgatt tggacatctt ctcgcggtca accatcacca ttccggagtg catccagtag
     2581 ttaacatact gaccatcatg ggcacaggtg gactgcgcga tttcattctc gtggtgcggg
     2641 aacatcaggt cagaaccgcc gccgtggata tcaaagtggt tacccagctg cttgcagttc
     2701 attgccgaac attcaatgtg ccagcctgga cggcccgcgc cccacggaga cggccagctc
     2761 ggttcgccct ctttcgacat cttccacaga acgaagtcca tcgggttgcg tttgtcgtcg
     2821 accacgtcaa cgcgcgcgcc tgcctgcagc tggtcgagat cctgacgcga cagcacacca
     2881 taagtcggat cggtcgggac gtcgaacatc acgtcgccgt tgtccgccgc ataagcgtga
     2941 cctttggcga tcagttgttc agtgagttca ataatttctg cgatatggtg cgtcgcgcgc
     3001 ggctccatat ccgggcgcag aatgttcaaa gcatcaaaat ctttgtgcat ttcggcgatc
     3061 atgcgatcca ccagcgccac aaagctttcg ccattttcat tggcacgttt gatgattttg
     3121 tcgtcgatat cggtaatgtt gcgcacatac ttcagtttat agccgaggaa acgcagatag
     3181 cgcgcaacca cgtcaaaagc aacaaaggta cgcccgtgac caatatgaca gagatcgtaa
     3241 acggtgattc cacacacata catgccgact tccccggcgt gaataggctt aaattcctct
     3301 ttttggcgtg tcagagtatt gaagattttt agcatcgaag attccgttta gacatgtgtg
     3361 ggtaattgag ttgcgtataa tacccatatt tcccgcgcga atcagcatac attgcgtgat
     3421 gatccgatcg tgcggttatg ctataacacc accctatata tgacccgaac tgggttgaag
     3481 caccaatcaa acggaacagg atgcaaaaat ggttactttc cacaccaatc acggcgatat
     3541 tgtcatcaaa acttttgacg ataaagcacc tgaaacagtt aaaaacttcc tggactactg
     3601 ccgcgaaggt ttttacaaca acaccatttt ccaccgtgtt atcaacggct ttatgattca
     3661 gggcggcggt tttgaaccgg gcatgaaaca aaaagccacc aaagaaccga tcaaaaacga
     3721 agccaacaac ggtctgaaaa atacccgtgg tacgctggca atggcccgta ctcaggctcc
     3781 gcactctgca actgcacagt tcttcatcaa cgtggttgat aacgacttcc tgaacttctc
     3841 tggcgaaagc ctgcaaggtt ggggctactg cgtgtttgct gaagtggttg aaggcatgga
     3901 cgtggtagac aaaatcaaag gtgttgcaac cggtcgtagc ggtatgcacc aggacgtgcc
     3961 aaaagaagac gttatcattg aaagcgtgac cgttagcgag taatcgtggc gacactcttt
     4021 attgcagatc ttcatctctg cgtggaagaa ccggcgatca ccgccggttt tctgcgtttt
     4081 ttagcggggg aagctcgcaa ggccgacgcg ctgtatattc ttggcgatct gtttgaagcg
     4141 tggattggcg acgacgagcc caatccactc catcgccaga tggcggcagc gatcaaagcg
     4201 gtgtccgatt ctggcgttcc ctgttatttc attcatggca accgtgattt tctgctcggc
     4261 aaacgctttg cccgtgaaag tggcatgacg ttattgccgg aagaaaaggt gctcgaactt
     4321 tatggccgcc gggtgttgat tatgcatggc gacacgctgt gcaccgatga cgcgggttat
     4381 caggcttttc gcgccaaagt ccacaaaccc tggctgcaaa tgctattcct cgccctgccg
     4441 ttgtttgtgc gcaaacgcat tgccgcgcga atgcgagcga acagtaaaga agccaacagc
     4501 agtaaatcgc tggcgatcat ggacgttaac caaaacgcgg tagtcagtgc gatggaaaaa
     4561 catcaggtgc aatggctgat ccacggacat acccatcgcc cggcggtaca tgaacttatc
     4621 gccaaccagc aacctgcttt tcgcgtggta ctcggtgcct ggcatacgga aggttcgatg
     4681 gtgaaagtca cggcggatga cgttgagctg attcattttc cgttttaaaa aacccgcaac
     4741 tttgctgatt tcacagccac gcaaccgttt tccttgctct ctttccgtgc tattctctgt
     4801 gccctctaaa gccgagagtt gtgcaccaca ggagttttaa gacgcatgtc ttcccgcaat
     4861 aatccggcgc gtgtcgccat cgtgatgggg tccaaaagcg actgggctac catgcagttc
     4921 gccgccgaaa tcttcgaaat cctgaatgtc ctgcaccacg ttgaagtggt ttctgctcac
     4981 cgcacccccg ataaactgtt cagcttcgcc gaaagcgccg aagagaacgg ttatcaggtg
     5041 attattgcgg gcgcaggtgg cgcagcgcac ctgccaggca tgattgccgc caaaacgctg
     5101 gtgccggtgc tgggcgtgcc agtacagagc gccgcactga gcggtgtcga tagcctctac
     5161 tccatcgtac aaatgccgcg cggcattccg gtgggtacgc tggcgattgg taaagctggc
     5221 gcggcaaacg cggcattact ggcagcacaa attctcgcga ctcacgataa agagctacac
     5281 cagcgtctga atgactggcg caaagcacaa actgatgaag tgctggaaaa cccggacccg
     5341 cgaggtacgg catgaaacag gtttgcgtcc tcggtaacgg gcagttaggc cgtatgctgc
     5401 gtcaggcagg cgaaccgtta ggcattgctg tctggccagt cgggctggac gctgaaccgg
     5461 cggcggtgcc ttttcaacaa agcgtgatta ccgctgagat cgaacgctgg ccggaaaccg
     5521 cattaacccg cgagctggcg cgccatccgg cctttgtgaa ccgcgatgtg ttcccgatta
     5581 ttgctgaccg tctgactcag aagcagcttt tcgataagct ccacctgccg accgcgccgt
     5641 ggcagttact tgccgatcgc agcgagtggc ctgcggtgtt tgatcgttta ggtgaactgg
     5701 cgattgttaa gcgtcgcact ggtggctatg acggtcgcgg tcaatggcgt ttacgcgcca
     5761 atgaaaccga acagttaccg gcagagtgtt acggcgaatg tattgtcgag cagggcatta
     5821 acttctctgg tgaagtgtcg ctggttggcg cgcgcggctt tgatggcagc accgtgtttt
     5881 atacgctgac gcataacctg catcaggacg gtattttgcg caccagcgtc gcttttccgc
     5941 aggccaacgc acagcagcag gcgcaagccg aagagatgct gtcggcgatt atgcaggagc
     6001 tgggctatgt gggcgtgatg gcgatggagt gttttgtcac cccgcaaggt ctgctgatca
     6061 acgaactggc tccgcgtgtg cataacagcg gtcactggac acaaaatggt gccagcatca
     6121 gccagtttga gctgcatctg cgggcgatta ccgatctgcc gttaccgcaa ccagtagtga
     6181 ataatccgtc ggtgatgatc aatctgattg gtagcgatgt gaattatgac tggctgaaat
     6241 tgccgctggt gcatctgcac tggtacgaca aagaagtccg tccggggcgt aaagtggggc
     6301 atctgaattt gaccgacagc gacacatcgc gtctgactgc gacgctggaa gccttaatcc
     6361 cgctgctgcc gccagagtat gccagcggcg tgatttgggc gcagagtaag ttcagttaag
     6421 caggtgctca tttgattgcc ggatgcggcg taaacgcctt atccggctta ccgctccggt
     6481 acccattgta ggcctgataa gacgcgtcaa gcgtcgcatc aggcatggtg caccgattgc
     6541 cggatgctct catcactaca gcgaaataca ggtccccgct tcgcccgcca gcgtctcttc
     6601 aattcgcgat aacgccccaa tccacgcggg tttaccacgg cttctgacat aaccactcac
     6661 cgccgttacc ttcggcccca tcgaaccatc ggctttggca aatggcgcta actcatccgg
     6721 tgtggcatgg cgaatggcac gttgctgcgg cgttccccag ttttcatata ccgcatcagc
     6781 atcggtgagg atcaccagtc catcggcatt aatctgctcg gcgagcaacg cagcggcgag
     6841 atctttatca atcactgctt cactccctgc cccatcttcc gtcacaggca cacctccgcc
     6901 gccactgcaa atcaccacat gcccctcttt gagcaacaac tcgatggctt cgctgtcgag
     6961 aattttacgc ggttgcggag acgccaccac ccggcgcaaa tatttaccat cacgtttcat
     7021 ctgccagccg taagccgctt ccagtgcctc ttgttcttct ggctgataaa ccggaccaat
     7081 aaatttctct ggctgcaaaa acgccggatc atcaggcgaa acctcaatgc gcgtcagcac
     7141 cgtcgtaaca ggcggcatct gcggctgtgc gctcaagctc tgcgccagca tatagccaat
     7201 catcccctgg ctttccgcca ccagcacatc cagcggatac ggttctacct ctttccacgc
     7261 cagattctgt aacgccagca gccccacctg cggcccgttg ccgtgaacaa tcgccagccg
     7321 ataagaacgg gccaggcgtg ccagcgcggg tacagcactg gcgatattgc gatattgatt
     7381 ttccgccgtc agcgcctcac cgcgctggag taaggcgttg cccccaagag ctacaaccag
     7441 tgttttcatg gttttccttg taataattgt tggccaagcc agaaccccag cagcgtatct
     7501 gcccctgacg tatgccccag cgccagcagc gaatcaatcg caacagcggt acgtttcggg
     7561 caactcagag catggacaaa gtgcaggagt ggcgaggcga aatatccttg cgcggcataa
     7621 cgtaaataac tgacgctcac cgctgtggta acgagttgaa gattgtcgga ataggcaaaa
     7681 aactggcgta actcagcagg cagcggatcg cctgccgcca acgccagtgg cccgaaaagc
     7741 ccggtttcct ccgcgcgttg catccatgca gctgcaagcg gttgaggatg cgcaggcggc
     7801 gtaatacgca gcaaacaata acgctgtggc tgtttaaccg tgaaacgccc gaggcgaatc
     7861 ccttgagcca caacctgtgg tcgttcattg ccgcataatc caccgcataa cgcatcgaat
     7921 tgcgcgcggc gaagcaccca tcctccgggg ccgaaaccac taccctgacg atgcagcgtt
     7981 aacagttcgc cgctttccgt catcaggtta atcgcccgtc gccacactcc cgctaaccgc
     8041 caggactggc gataattcgg tgcactacgg ctggcaagca gaggatggat gatcgtcatt
     8101 gcgcgttcct caaccaatcc ccatgctttc agccagtgct tccagcgcct gttcaaaaca
     8161 cgccagcggt gcccgcacag tgcctgcgcc aatctgcccg atccccgcct ctttatgggc
     8221 gataccggta ttgatgagtg gcgtaatgtc ggtttctacc acgcgacgaa tgtccagtcc
     8281 caggcacgcg ccctgaaaat cccagcctgg gatctgcaac tgcatattgc gctcaaggta
     8341 aatttccgcc atctcttcag ataccgctct ggccgcttcc atgccacccg caccgacaaa
     8401 gcgcgttacg ccaggcgctg cgatcattgc cgcacctccg ataccaaagg tttcggtaat
     8461 cgcgctatcg cccatatccg ggttcgcctg ctcctgcgag aagccggtga aaaacagacc
     8521 ttgcggagtg tttacaggcg cagtaaacca gcgttcgccc agcccgctta cccgaatccc
     8581 gaacatattg ccgttgcggg tcattgccgt gacgatgctg cctgcgcgga tcatcgcgcc
     8641 agcatccatc gccgccttgc agtaagccat cgcgaggttg aggaagaact gatcggtcac
     8701 gctgaggaaa tccatcactt cggcgatgtg ctgtttatca tgatcgaggc gagcaatttg
     8761 tggggccagc gcacgcatta acagtgcaga ggaagcaata ttgcgttgat ggaactcatc
     8821 gcccatcgta atgccctgcg ccatcatcgc cgtgagatcg ataccgcgct ccatgcgccc
     8881 cagcgccgcg cttaatactg gcattaacac atcgcgcatc cagcgatggc gagtcaggac
     8941 atcttcgccg taagcgccaa aacgcatcac tttgccgata ccttcgttga ggttgcagta
     9001 cgcccggttg ccgtcggtca cgttctcgac caccagcatc ggcatactgg cagaagtaat
     9061 accgcccatt ggaccgacgg cattcacatg gtgacaagga atgaagttca cttccccctg
     9121 ctccagtatt gccagcgcct gcgcttcatc tttcgcccag ccttcgaaca gacatgcgcc
     9181 cacgcacgcc cctttcatgg gtccggtcat ctcctgccag cgcattggcg gcccggcgtg
     9241 aagcagtgtt ttgccctcgt ttagttcgct gataagtgaa gaagccggtt gcacatccag
     9301 ccagtgtgga cgagcgcgac gaatttgttc gataaccgca gcattggctt gcgccactga
     9361 tgtaaacatg ggaacccctt attgcaaacg ttctaataaa cgagccaggt aatgactcca
     9421 acttattgat ag
//
LOCUS       DAEEKQ010000150         9419 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.152, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000150 DAEEKQ010000000
VERSION     DAEEKQ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9419)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9419)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9419
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.152"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..168
                     /locus_tag="KLK57_003371"
     CDS             <1..168
                     /locus_tag="KLK57_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0893657.1"
                     /translation="NRRLDFCADAIRHAADDEKLAGIGFHWGFSDQSHFSTVFKQRFG
                     MTPGEYRRKFR"
     gene            complement(340..669)
                     /locus_tag="KLK57_003372"
     CDS             complement(340..669)
                     /locus_tag="KLK57_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HBE0893658.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(674..859)
                     /locus_tag="KLK57_003373"
     CDS             complement(674..859)
                     /locus_tag="KLK57_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HBE0893659.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(856..3495)
                     /locus_tag="KLK57_003374"
     CDS             complement(856..3495)
                     /locus_tag="KLK57_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HBE0893660.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGMVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVIAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALISGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHEYELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPINATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3703..4692
                     /gene="ldhA"
                     /locus_tag="KLK57_003375"
     CDS             3703..4692
                     /gene="ldhA"
                     /locus_tag="KLK57_003375"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HBE0893661.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4803..5225
                     /gene="hslJ"
                     /locus_tag="KLK57_003376"
     CDS             4803..5225
                     /gene="hslJ"
                     /locus_tag="KLK57_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HBE0893662.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(5222..5488)
                     /locus_tag="KLK57_003377"
     CDS             complement(5222..5488)
                     /locus_tag="KLK57_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HBE0893663.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            5762..9286
                     /gene="nifJ"
                     /locus_tag="KLK57_003378"
     CDS             5762..9286
                     /gene="nifJ"
                     /locus_tag="KLK57_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HBE0893664.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPCVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQTLPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGKRETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTQPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARKCGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT         2343 a         2460 c         2304 g         2312 t
ORIGIN      
        1 aaccgtcgtc tcgatttttg tgcagatgcg attcgccatg ccgcagatga tgaaaaactg
       61 gcaggcatcg gctttcattg gggattttct gaccagagtc atttttcaac ggtatttaag
      121 caacgctttg ggatgacgcc aggcgagtat cgacgtaaat tccgttaact aattcgcgtt
      181 gtgtgtactg caacgccaat tagttaaaac agatatttga ctatgcattt ttcagggcaa
      241 aaggcaattt gacaggagtt tttgaatatt caccttgttt caggaaaata gatcaatcgc
      301 tttctcctgt agcgttatcc cgatattgcg gttttgctat taaaattttc gcacccattt
      361 accgtttatc ccttgtacat attcccccgg tttggcccga gctacgagct tttgcccggc
      421 gagtttcgcg atatcatcta ccgacacatt attttgcttt gccagttgtt ggtaactcgc
      481 tttacgttcg gcattaatgt cggttactaa tttctctgtt tcagcatccg ttttcagcgc
      541 aaccagataa ccataaaatg tttcacctac ccgcccctga gttctggctt catccagagt
      601 caatgccatt acattgctgc ttaccagccc aacaagaaac gcacaaagaa gtaatgtttt
      661 cttcatcatc gcctcagaaa agatcgctac gagtttcaag cagctcttcg acatctttgt
      721 ctgccttgat gatgatctca tgctcaattt taacgttcat attgatagtg atcggttcct
      781 tgggcgcagc gacttcaata cgcggcgtac agccaaccag cataaaagat gacgtcaacg
      841 cagccagtaa aattttcatt gtttttcctc acactctttt ccttgcggac agtcatttcc
      901 cggcagacgt gcgttctgct ccaaccatgc ctggagatta tcgccaaagc gtaaactgcg
      961 ccacagcgta aaaatattct cttcatgatg gtaattaagg tttaccgtac cgcttttacc
     1021 atcaacgcga ctggtacctg taatgttggc ctgcatggtt aataaaccga gattatctaa
     1081 gttaattttt gtcgatgacc ggctaatttc catatagcgc aaccagttaa ttgctgaacc
     1141 cgcagtcata ttgtctttca ccaccgcatc tgcggtgtct ttatccagcc gcaaggtcat
     1201 tggcccgcta tttgccagcc aaccatcttt cactatccat ttttcgttgt ttagccataa
     1261 cggcaatgca ccactaaatg ccccggacat ggcgaattgt ttcggattga cggcgctaac
     1321 cagttcgctg gatgagagat tattcagccg caacagcgcc gggtcatgtt gcggcatacg
     1381 taattgtttc atcagtacgt taccgcctaa cacatcgacg ctaacatcag tcaacagcaa
     1441 gggttcttct tcagtccacg gataacgccc ttgcaaatca gccgtaatat ttttcgctgt
     1501 caccagatta atcacttcgg caattcgcaa cgtaacgggg ccgcgagtcc ccagatgcca
     1561 ggctccatcg gcaaaacgga aaggcaggac aaaatcgaca ccgttaacct gattatctgg
     1621 catccaggca ctaccgcctt tcaacacgcc gtgtcctccc gcgcggaatc cttgttcagg
     1681 cgcagcagaa aatgcaacct gagcatacag ttccccatcg cgtaagttca tcttccagtc
     1741 gggtggcacc agcggctgga atacagtcag tgattgtttt ggccaccagg cgttaccgcg
     1801 cagacgaata ccgtcccagc gaccatttac ccgaaccggg ccaatctcac cagcatgtaa
     1861 atcgccttta aagaggaaat acgtcggatc gcgcccatcg acgctaaatt ttaaggttga
     1921 tggcggtaac acactgccgc cagtgaacag cgtttgcccg gcgtccagtg acagcgcgcc
     1981 gctgaaggag ggatgctgtg cgtcacgtac ccagacgacg ggcttgtcga gaattaatcg
     2041 cggcttttct accgtcatcg taccgtattg taactggtcg aaaccggtgg agaggtcggt
     2101 cagcgtaatc gtgctgtcat gccattcacc ttttcctgcg acatcccagg tggcatttat
     2161 cggtgtaaaa ctgccttttc cccagtagcg ccactgccag cggcccgcgt cagggagaaa
     2221 atcattcgcc agcccatcca tatgcagcac gaaatcgccc agttcatatt catgcgcctg
     2281 caaaatggcc tgcaaacgtc cgtcaacacc acgttgggtg acttttacac ccgctaaagg
     2341 ccagcggatt tcatcgatgt ccagcgaatc gatgactctt cccttcgaac gtaataacgc
     2401 gcctggctca aaggccagcg ttgggtcagt cagacttcca cttagctgcg cgggtaagcg
     2461 ggcatataaa atgaggtccg cctgtttcgc ttcaccggtc agctgcagag gcagctggct
     2521 gttatccatg cttaattttc ctgggccaaa atttagcacc gcgttgcctt tacccgcttg
     2581 cccctgggtc agcacactca gtcgtccgct gatcagagcg ttctcaagcc ctgcctgcca
     2641 gttgtcgact ttgacaccca gtcggccact caaaggaaaa cctgcatacg gccaactcca
     2701 gcgaccatcg cttacggtca attgttgacg agtaatttgc cacggcaaat cgagcaacgg
     2761 atcgccgtta tcccgtgcca gcacaatcaa ttgcccgcta ttttcctgcc agtccagctc
     2821 tgcatccacc agtgacggtt cctgcggcaa gtttaacgtc gcagtagcat gcccacttac
     2881 aggaagtcca tccggcacga gcggcatagt aaattccccc accagtttaa ccggcggctg
     2941 attttcaaac gcgatgacat ccagttcgct gactgtaagt tgttgccctt tcagctggcc
     3001 ttgaaattta actttttcgc cctgataacg cagttgctga atatcagagg ttaatgcgag
     3061 agagagtttt ccctgccatt cctgccaggg agaaaaaatc agtttatcga tattgatcca
     3121 ggtgttaggc agcatggact gccactgcgc gagggttttt ggagcagctg gcgattgctc
     3181 cgtctgcggc aatttcgcca gacaagcaga atcaagttct accatgccga cgttcaataa
     3241 ccagcggctg ggatgtgaaa ggctggcgtt ggtgatatgc gcaagctgac aatctcccac
     3301 cagataacgg agatcgggaa tgattaaacc tttacgcgta atgcgtgggc tttcatctaa
     3361 tgcaatacgt gtcccgagcg gtagccagat gcccgccagc gtgggaaccc acagcccgag
     3421 cgtcatcagc agcgtcaacg gcacaagaat aatcagtaat aacagcgcga gaacggcttt
     3481 atatttaccc agcatgggta gttaatatcc tgatttagcg aaaaattaag cattcaatac
     3541 gggtattgtg gcatgtttaa ccgttcagtt gaaggttgcg cctacactaa gcattgttgt
     3601 tgatgaattt ttcaatatcg ccatagcttt caattatatt tgaaattttg taaaatattt
     3661 ttagtagctt aaatgtgatt caacatcact ggagaaagtc ttatgaaact cgccgtttat
     3721 agcacaaaac agtacgacaa gaagtacctg caacaggtga acgagtcctt tggctttgag
     3781 ctggaatttt ttgactttct gctgacggaa aaaaccgcta aaactgccaa tggctgcgaa
     3841 gcggtatgta ttttcgtaaa cgatgacggc agccgcccgg tgctggaaga gctgaaaaag
     3901 cacggcgtta aatatatcgc cctgcgctgt gccggtttca ataacgtcga ccttgacgcg
     3961 gcaaaagaac tggggctgaa agtagtccgt gttccagcct atgatccaga ggccgttgct
     4021 gaacacgcca tcggtatgat gatgacgctg aaccgccgta ttcaccgcgc gtatcagcgt
     4081 acccgtgacg ctaacttctc tctggaaggt ctgaccggct ttactatgta tggcaaaacg
     4141 gcaggcgtta tcggtaccgg taaaatcggt gtggcgatgc tgcgcattct gaaaggtttt
     4201 ggtatgcgtc tgctggcgtt cgatccgtat ccaagtgcag cggcgctgga actcggtgtg
     4261 gagtatgtcg atctgccaac cctgttctct gaatcagacg ttatctctct gcactgcccg
     4321 ctgacaccgg aaaactacca tctgttgaac gaagccgcct tcgatcagat gaaaaatggc
     4381 gtgatgatcg tcaataccag tcgcggtgca ttgattgatt ctcaggcagc aattgaagcg
     4441 ctgaaaaatc agaaaattgg gtcgttgggt atggacgtct atgagaacga acgcgatcta
     4501 ttctttgaag ataaatccaa cgacgtgatc caggatgacg tattccgtcg cctgtctgcc
     4561 tgccacaacg tgctgtttac cgggcaccag gcattcctga cagcagaagc tctgaccagt
     4621 atttctcaga ctacgctgca aaacttaagc aatctggaaa aaggcgaaac ctgcccgaac
     4681 gaactggttt aatcttgccg ctcccctgca ttccagggga gcttattcag ataatcccca
     4741 aagacctttc atcttctatt cttaaaatag ccctgagtca gaaactgtaa ttgagaacca
     4801 caatgaagaa agtagccgcg tttgttgcgc taagcctgct gatggcggga tgtgtaagta
     4861 atgacaaaat tgctgttacg ccagaacagc tacagcatca tcgctttgtg ctggaaagcg
     4921 taaacggtaa gcccgtgacc agcgataaaa atccgccaga aatcagcttt ggtgaaaaaa
     4981 tgatgatttc cggcagcatg tgtaaccgct ttagcggtga aggcaaactg tctaatggtg
     5041 aactgacagc caaagggctg gcaatgaccc gtatgatgtg cgctaacccg cagcttaatg
     5101 aactcgataa caccattagc gaaatgctga aagaaggtgc acaagtggat ctgaccgcga
     5161 accagttaac gctggcgacc gcgaaacaga cattaactta taagctggcg gatttaatga
     5221 attaatagct gccacagctc ccggcggcaa gtgactgttc gctacagcgt ttgccgttgg
     5281 gtaatgcaca catcccaatc gccgtaccat ccagttgacg ggcaacagaa agcgaaccgc
     5341 cgatcattgc acaatttgct tctccactac tggacatcga cgcttttaaa cctggcgcta
     5401 cgtgcgccgc agtcgcctgc tgaacaggtt cactactaca cgccgacaac aataaagcgg
     5461 cacaccctac ccaaaacgct gctcgcatct cttcttcctc tgatcttcaa gccaaacgac
     5521 accgccataa ataataggca gcacagaggg cggcgtcgag agctgtcctg cgcgttgccc
     5581 cgcattttta cttttttatg gctatttttt tgccctctgt ttgatcaaaa cattcattac
     5641 gctgatgtgg gggacacaaa agcgaaaatg cagaagaaag ccatttgcta aaattgaagg
     5701 attactactg ggcgcgcagc aatttcgtgc gcccctcatt tgcgcaatgt aagggtgtca
     5761 tatgattact attgatggta atggcgcggt tgcttcggtg gcgtttcgca ccagtgaagt
     5821 tatcgccatc taccctatta cccccagttc cacgatggca gaacaggctg atgcctgggc
     5881 cggaaacggc ttgaagaacg tttggggaga cacaccatgc gtggttgaaa tgcagtcgga
     5941 agcgggtgct atcgctaccg tgcatggcgc tttgcagacg ggtgcccttt caacatcgtt
     6001 tacgtcatcg cagggtttgc tgctgatgat cccgacactg tacaaactgg caggcgaact
     6061 cacaccgttt gtcctgcatg tagcggcacg taccgttgcc acacatgcac tctctatttt
     6121 tggcgatcat tccgacgtca tggcggtgcg ccagacgggt tgcgcgatgt tgtgtgcggc
     6181 aaacgtccag gaagcgcaag actttgctct catttcgcac atcgcgacgc tgaaaagccg
     6241 cgtgccattt attcatttct ttgatggttt ccgcacgtcc cacgaaatca acaaaattgt
     6301 cccgctggcc gatgacacga ttcttgatct catgccgcag gccgaaattg atgctcatcg
     6361 cgcccgggcg ctcaacccgg aacatccggt gatccgtggt acgtccgcca atcctgacac
     6421 ttatttccag tctcgcgaag ccaccaaccc ctggtacaac gcagtctatg accatgttga
     6481 acaggcgatg aatgatttct ctgccgcgac aggtcgtcag tatcagccgt ttgaatatta
     6541 cgggcatccg caagctgaac gggtgattat cctgatgggc tcagccattg gcacctgtga
     6601 agaagtggtt gatgaattgc taacccgtgg cgaaaaagtc ggcgtgctga aagttcgcct
     6661 gtaccgcccc ttctcagcca aacacttgct acaaactcta ccgggatccg tacgcagcgt
     6721 ggcggtactg gacagaacca aagaacccgg tgcccaggca gaaccgctct atctggatgt
     6781 gatgaccgca ctggcagaag cctttaataa tggcaagcgc gaaactctgc cccgtgtcat
     6841 tggtgggcgc tatggtcttt catccaaaga atttggcccg gactgtgtac tggcggtatt
     6901 tgccgagctc aacgcggcta aaccgaaagc gcgctttacg gttggtattt acgatgatgt
     6961 aaccaatctg tccctgccgt tgccggaaaa cacccagcca aactcggcga aacttgaagc
     7021 cttgttttat ggccttggta gtgatggcag cgtttccgcg accaaaaaca atatcaagat
     7081 tatcggtaat tccacgccgt ggtacgcaca gggctatttt gtttacgact ccaaaaaagc
     7141 gggcggcctg acggtttctc accttcgcgt gagcgaacag ccgattcgtt ccgcttatct
     7201 catttcccag gctgattttg ttggctgcca ccagttgcag tttatcgata aatatcagat
     7261 ggctgagcgt ttaaaacctg gcggcatttt cctgctcaac acgccgtaca gcgcagatga
     7321 agtgtggtcg cgcttgccgc aagaagttca ggccgtgtta aaccagaaaa aagcgcgctt
     7381 ctatgtgatt aacgcggcga aaatcgcccg caaatgtggc ctggcggccc gtattaatac
     7441 cgtcatgcag atggcttttt tccatctgac gcaaattctg cctggcgata gcgccctcgc
     7501 agaattgcag ggtgcgattg ccaaaagtta cagtagcaaa ggccaggatc tggtggaacg
     7561 caactggcag gctctggcgc tggcgcgtga atccgtagaa gaagttccgt tgcaaccggt
     7621 aaatccgcac agcgccaatc gaccgccagt ggtttccgat gccgcccctg atttcgtgaa
     7681 aaccgtaacc gcagcgatgc tcgcagggct tggtgatgcc ctccccgttt cggcgctgcc
     7741 gccagacggc acctggccga tgggcaccac tcgctgggaa aaacgcaata tcgccgaaga
     7801 gatccccatc tggaaagagg aactctgtac ccaatgtaac cactgcgtcg cagcttgccc
     7861 acactcagct attcgcgcaa aagtggtacc acccgaagcg atggaaaacg cccctgccag
     7921 cctgcattcg ctggatgtga aatcgcgtga tatgcgcggg cagaaatatg tcttgcaggt
     7981 ggctccggaa gattgcaccg gttgtaacct gtgcgtcgaa gtttgcccgg cgaaagaccg
     8041 tcagaatcca gagattaaag ccatcaatat gatgtcgcgc ctggaacatg tcgaagaaga
     8101 gaaaatcaat tacgatttct tcctcaacct gccagaaatc gaccgtagca aactggaacg
     8161 tattgatatt cgtacatcgc agctgattac accgctgttt gaatattccg gtgcttgctc
     8221 cggttgtggc gagacgccgt atattaaatt gctgactcag ctctatggcg accggatgtt
     8281 gatcgctaac gccaccggct gttcttcaat ttatggcggt aacctgccct ctacaccgta
     8341 taccaccgat gccaacggtc gtgggccggc atgggcgaac tctctatttg aagataatgc
     8401 cgaatttggc cttggtttcc gcctgacggt cgatcaacac cgtgtccgcg tgctgcgtct
     8461 gctggatcaa tttgccgata aaatcccggc ggaattactg acggcgttga aatcagacgc
     8521 tacgccagag gttcgtcgag aacaggtcgc agctttacgc cagcaactca acgatgttgc
     8581 cgaagcacat gaactgctac gtgatgcaga tgcactggtg gaaaaatcaa tctggctgat
     8641 tggtggtgat ggctgggctt acgatatcgg ctttggcggt ctggatcatg tattgagttt
     8701 gacggaaaac gtcaacattc tggtgctgga tacgcaatgc tattccaaca ccggtggtca
     8761 ggcgtcgaaa gcgacaccgc tgggtgcagt aactaaattt ggcgagcacg gcaaacgtaa
     8821 agcgcgtaaa gatcttggcg tcagtatgat gatgtacggt catgtttatg tggcgcagat
     8881 ttctctcggc gcgcagctga accagacggt gaaagcgatt caggaagcgg aagcgtatcc
     8941 gggaccatcg ctgatcattg cttatagccc gtgtgaagag catggttacg atctggcact
     9001 cagccatgac cagatgcgcc aactcacagc taccggcttc tggccgctat atcgcttcga
     9061 tcctcgtcgt gccgatgaag gcaaactgcc gctggcgctg gattcacgcc cgccgtcaga
     9121 agcactggaa gaaacgttac ttcacgagca acgtttccgt cgtctgaatt cgcagcagcc
     9181 agaagtggca gaacagttat ggaaagatgc tgcagctgat ttgcaaaaac gctatgactt
     9241 cctggcacaa atggccggaa aagcggaaaa aagcaacacc gattaaatgc tctggataag
     9301 gattacccaa ttctaaaaaa aagcccggac gactgttcgg gcttgtcttt ttatatgttg
     9361 gaaaatcagt ggcaatgcaa tggcccaaca gaaaataaaa tctgtgacaa taaaggcat
//
LOCUS       DAEEKQ010000151         9397 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.153, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000151 DAEEKQ010000000
VERSION     DAEEKQ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9397)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9397)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9397
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.153"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(350..1603)
                     /locus_tag="KLK57_003379"
     CDS             complement(350..1603)
                     /locus_tag="KLK57_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0893665.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFSSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            1710..2603
                     /locus_tag="KLK57_003380"
     CDS             1710..2603
                     /locus_tag="KLK57_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0893666.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPASRNFRIHLLNALR"
     gene            2738..3958
                     /gene="mlc"
                     /locus_tag="KLK57_003381"
                     /gene_synonym="dgsA"
     CDS             2738..3958
                     /gene="mlc"
                     /locus_tag="KLK57_003381"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HBE0893667.1"
                     /translation="MVAENQLGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            4083..4778
                     /gene="bioD"
                     /locus_tag="KLK57_003382"
     CDS             4083..4778
                     /gene="bioD"
                     /locus_tag="KLK57_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBE0893668.1"
                     /translation="MLERFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEVIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="KLK57_003383"
     CDS             complement(4731..6023)
                     /gene="clcB"
                     /locus_tag="KLK57_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HBE0893669.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYSVQLSVTVQASDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="KLK57_003384"
     CDS             complement(6182..6796)
                     /gene="dmsD"
                     /locus_tag="KLK57_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HBE0893670.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(6839..7693)
                     /locus_tag="KLK57_003385"
     CDS             complement(6839..7693)
                     /locus_tag="KLK57_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HBE0893671.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFMASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="KLK57_003386"
     CDS             complement(7695..8312)
                     /gene="dmsB"
                     /locus_tag="KLK57_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HBE0893672.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDEPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTSDTTGYLANPEEV"
     gene            complement(8323..>9397)
                     /locus_tag="KLK57_003387"
     CDS             complement(8323..>9397)
                     /locus_tag="KLK57_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HBE0893673.1"
                     /translation="RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSK
                     CEMIVGIDHFMTASAKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFER
                     KPIYWMLSEVAKRLGPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIF
                     KKKCPEEHYVAFRAFREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEVIHPLPA
                     YTPGFDGWDDPLRKTYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARG
                     IRHGDTVRVFNNNGEMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILT
                     SHRPSPLAKGNPSHSNLVQIEKV"
BASE COUNT         2449 a         2379 c         2437 g         2132 t
ORIGIN      
        1 cagacttccg tgagtggttg gtttcagcgt gtaacgatat gtacaaacgc tgaataaatt
       61 acagcattga taataacgta ttgtgactta agggaaattt agctaccaat aatagtagtc
      121 ttgatcgggt taactatttt actacttaca aagcggtaga ataactgcgc atcagtaata
      181 aatgacacac agcaaaatga atccgtttat ttgggtactt ataatcctga tgacgctaga
      241 cgcgctgcgg gaattggctg gcgcttcgtc tattttagga tggctattaa cgttggtttg
      301 agcaagcaat aagtccggac gtgtagttac cgcagtccgg gcttattttt caggcgtgca
      361 gacgacgatg caaacgcgtc ccgaccagca gagcaatgac cagcatcaga gcaataaatg
      421 cgccgacgcc gttccagcca tagttatgcc agaaaacacc acccagcgtc ccggcaatac
      481 tcgaccccag atagtaactg aacagataca gcgaggaggc ctggccttta gcgcgttttg
      541 cgcgggggcc aatccagctg ctggcaactg agtgggctgc gaagaatcct gctgagaaga
      601 gtaacattcc ggcaaagatc agccacagcg agctgaataa ggtcatcagt aaaccaaaca
      661 gcataacccc cgtcgaaaac aacatcaccg gaccgcgccc gtagcgggtg gtcatggttc
      721 cggctttggg tgagctccat gtaccggtca aataagccag cgataataag ccaaccacgg
      781 cctgactgac atgccagggg gaaagcatca accgatagcc gatgtaatta aacagcgtga
      841 cgaacgaccc catcagcaaa aagccttctg cgaacaataa cggtaatccc cggtcacgcc
      901 agtgcagacg aaagttgata aataacgtct tagggcgcag cgaagtcggg cgaaaatggc
      961 gtgattccgg aaggattttc cagaacatca acgccgaggc tagcgcgaaa caaccgattg
     1021 ccgccagagc aattcgccag ttgaaaaagt ccgtgaagac accactaatt aagcgtccgc
     1081 tcatgccgcc aattgagttg ccgctgatat acaaccccat cgaaaaggcc acgaaactgg
     1141 ggtggatttc ctcgctaaga taagtcatgc caacagctgc cacgccactt aacgaaagcc
     1201 caatcaaggc gcgcataatc aaaatgccgt gccagctggt catcattgtc gaaagtaacg
     1261 tacaaatgga ggccaacagt agcgccgtga ccatcactgg tttgcgacca atggcatcgg
     1321 atagggggcc agtaaacagc aaaccaatag ccaacatcgc cgtggaaatg gacagtgaaa
     1381 tactactgtt cgcgggggtt aagccaaact cctgcgaaag caccggaagg ataggctgca
     1441 cacaatagag gagtgcaaag gttgccagtc cggcagagaa cagcgccagg gtgacgcgca
     1501 taaattgcgg cgtaccgcgt ttaataaatt gatttggctg agaaatgctt tgcttgtcag
     1561 tgtcgcttgc cggagcgcca tcaacagttg tagtacggct cacttgaaat ccttgctaaa
     1621 tatgcctgtg gatcaggctt atacataggt taggaaaatc gaattgttct gtctaatata
     1681 ttaataatct caaataagat gttttaaata tgaatattga acttcgtcat ctgcgttact
     1741 ttgttgctgt tgcggaagag ctgcatttcg ggcgcgccgc tgcccgcctg aatatttcgc
     1801 aaccaccgct aagtcagcag attcaggcac tggagcaaca aattggtgcc cgactgctgg
     1861 cacgaactaa tcgcagtgta ttgctgacgg cagcaggaaa acagtttctt gcagatagtc
     1921 ggcaaatcct gtctatggta gatgacgctg ccgctcgcgc tgaaagactg catcagggtg
     1981 aagcggggga gttgcgcatt ggttttactt cgtccgctcc ttttattcgg gcggtgtccg
     2041 atacgttatc gctgtttcgc cgtgattatc ccgatgtcca tttacaaacc cgcgaaatga
     2101 acactcgtga gcaaatcgct ccgctcattg aaggaacgct ggatatggga ttgctgcgta
     2161 acacagcgtt accggagacg cttgaacacg cagtcatcgt ccatgaaccg cttatggcga
     2221 tgatcccgca cgatcatccc ctggcaaata acccgaatgt aacgctggct gaactggcga
     2281 aagaaccctt cgtctttttt gatccgcacg tcgggacagg gctgtatgac gatattctcg
     2341 ggctgatgcg acgttaccat ttgacgcccg tcatcactca ggaggtgggc gaggcaatga
     2401 ccatcatcgg tctggtttcc gccggtctgg gtgtttcaat tttgcctgct tcatttaaac
     2461 gtgttcagct caacgaaatg cgctgggtgc cgattgctga agaggatgcg gtttctgaaa
     2521 tgtggttggt ctggccgaaa catcatgaac aaagtccagc ttcgcgtaac tttcgtattc
     2581 atctgctgaa tgctctcagg tgagggaaat ttcagcgaaa aagcccgaaa aatgtgctgt
     2641 taatcacatg cctaagtaaa aatttgacga cacgtattga agtgcttcac catagcctac
     2701 agattatttc ggagcgcgaa aatataggga gtatgcggtg gttgctgaaa accagcttgg
     2761 gcacattgat caaataaagc agaccaacgc gggcgcggtt tatcgcctga ttgatcagct
     2821 tggtccagtc tcgcgtatcg atctttcccg tctggcgcag ctggctcctg ccagtatcac
     2881 taaaattgtc cgtgagatgc tcgaagcaca cctggtgcaa gagctggaaa tcaaagaagc
     2941 ggggaaccgt ggccgtccgg cggtggggct ggtggttgaa actgaagcct ggcactatct
     3001 ttctctgcgc attagtcgcg gggagatttt ccttgctctg cgcgatctga gcagcaaact
     3061 ggtggtggaa gagtcgcagg aactggcgtt aaaagatgac ttgccattgc tggatcgtat
     3121 catttcccat atcgatcagt tttttatccg ccaccagaaa aaacttgagc gtctaacttc
     3181 gattgccata accctgccgg gaattattga tacggaaaat ggtattgtac atcgcatgcc
     3241 gttctacgag gatgtaaaag agatgccgct cggcgaggcg ctggagcagc ataccggcgt
     3301 accggtttat attcagcatg atatcagcgc atggacgatg gcagaggcct tgtttggtgc
     3361 ctcacgcggg gcgcgcgatg tgattcaggt ggttatcgat cacaacgtgg gggcgggcgt
     3421 cattaccgat ggtcatctgc tacacgcagg cagcagtagt ctcgtggaaa taggccacac
     3481 acaggtcgac ccatatggta aacgctgtta ttgcgggaac cacggctgcc tcgaaaccat
     3541 cgccagtgtg gacagtattc ttgagctggc acagctgcgt ctcaatcaat ccatgagctc
     3601 gatgttacat gggcaaccgt taaccgtgga ctcattgtgt caggcggcat tgcgcggcga
     3661 tctactggca aaagacatca ttaccggggt gggcgcgcat gtcgggcgca ttcttgccat
     3721 catggtgaat ttatttaacc cacaaaaaat actgattggc tcaccgttaa gtaaagcggc
     3781 agatatcctc ttccctgtca tctcggacag catacgtcag caggcccttc ctgcgtatag
     3841 tcagcacatt agcgttgaga gtactcagtt ttctaaccag ggcacgatgg caggcgctgc
     3901 gctggtaaaa gacgcgatgt ataacggttc tttgttgatt cgtctgttgc agggttaaca
     3961 ttttttaact gttctaccaa aatttgcgct atctcaattt gggccaggaa agcataactt
     4021 agactttcaa ggttaattat tttcctagtt tatatttgtg aagcataacg gtggagttag
     4081 tgatgctgga gcgtttcttt attaccggta cagacacttc tgtagggaaa acggtggttt
     4141 cccgcgcatt gctacaagcg ttagcctccc agggaaaaac agttgcggga tataaacccg
     4201 tagcgaaggg gagcaaagag acacccgaag ggctgcgtaa taaagatgcc ctggtgttgc
     4261 agagtgtttc aaccatcgaa ctgccttatg aagcagttaa tcctatcgcg ttaagcgaag
     4321 aagaaagtag cgtggcgcac agttgcccaa tcaattacac cctcatttca aacggcctgg
     4381 caaacctgac cgataaagtc gatcatgtcg tggtagaagg gactggcggc tggcgcagtc
     4441 tgatgaatga tttgcgtcca ctctctgaat gggtggtgca ggaacagttg ccggtgttga
     4501 tggttgtcgg tattcaggaa ggttgcatta accatgcact gctaacagct caggcgattg
     4561 ccaacgacgg gctgccgctc attggctggg tggctaaccg aatcaaccca ggactggcgc
     4621 attatgcgga agtcattgat gtgctgggta aaaaacttcc ggcaccgctc attggtgaac
     4681 tgccttatct gccgcgcgcc gaacagcgag aactggggca atacatccgc ttagctatgc
     4741 tgcgcagtgt gctggcggta gatagagtca cggtgtaacg tccgcgaaat taccgacgca
     4801 atgacgcagg caatcaataa accggggagt agctgatact ccccggtcat ttcacatatc
     4861 atcaacgtcg acataatcgg cgcgtgcgtg gttgccgcca acagtgtcgc catcccggtc
     4921 aatccgagta aaagcgtaat ttcttcgcca tcagggaacc ataatcccag gctacgacca
     4981 tacaacatgc caatggcaag accgataaat agcgtcggtg taaagacacc accgggtgcg
     5041 ccggaaccgc tactcgccag cacggcacac agtttacaaa ggaagatccc ggcaatgatc
     5101 attaacagtg gtggggcggt taaaaaggat tgtacggtgc tatagccgtt gccccacact
     5161 gcaggtgtga acagggaaag cagacccacg atcaacccgc ctagtgccag ttgccagggc
     5221 ggcgcaagtt tgagactcac aaatccacga tgacaggcgt tcattaacgt taacaacagt
     5281 ggtccgcaca gacctgccag cacacctgta ctgataatca gcgcatagtc actagcctga
     5341 accgttactg agagttgtac gctgtagagt aacgcgtcgc tatgattaat aagattgcta
     5401 accagcaacg ccacgacggc ggaaataatc accgggccga gagaggccaa catcatagtg
     5461 ccaaacagca cttcggctat aaataaactg ccagcaagcg gggcgcgata ggccgcagcc
     5521 attcccgccg cggccccaca ggcgatccat aatttccatt cctggcgtgg cgtaaaacgt
     5581 tgggcaaaac aggaggcggc aagggcagct aaaagaatca tcgcaccttc gcgaccaatt
     5641 gcactgccgc tggttactac cagcagcgag gcaagcgatt taaccaggct tgctgcgtaa
     5701 tcgaactgtc catcggtttg caacgcttcc atgtaatcgg tcggcgcatg agggcgttgt
     5761 tgggtaaatt tctgccagcc catcagcaac aaacccgccg ccagtccgcc gagcgccgga
     5821 gttagcagcc gtcgccaggg ggaaaggttt gtcgctgcat tgaccagact gccggagtca
     5881 ttattgagga acaaccactc cagtagcagc atcgcatgac gaaacccggc aacggcaaag
     5941 gccgcgagaa taccgacgac tgttgcgata agcagacggc ggaacatagt gcgcagatcc
     6001 ggatagatgt gtagatgatg catgagcaga aatgggcaat gaaacacggg cgactatttt
     6061 gcggcaaaag aggccaaccg caaaagcagt gcgtgcttta agagataaaa aaacgtgaca
     6121 ttgtaactta ttattgccga atcgcgcgta atcgtcacca tccggcaata ttacggtgat
     6181 cctatcgaaa gagtggttta accgcgacag gaatcaacag ttgtgattgc cattgcgcca
     6241 gcgttaagcg agccagttca cccagtgcac ggtagaaagg gtgttctgct ttttcgataa
     6301 aaacatcaag aaaacgtgtt gaccacggaa aaaggtgcca tgccagcaat tcttcgcatt
     6361 ctgtctggcg accattctcc gccaaccacg cagccatcaa cagcagtgaa ccaaaatgat
     6421 cttccggttc gttttgcttc atttcaaact gaatgccttt ctcgcgcatc cactgacgaa
     6481 gtgccaatgt tgaatcgcca aacagcacag attcgcgatc cagccagacc gatccccacg
     6541 gcggagacgg cagtgcccac gggccgacaa acaaacgctg ccaggcctgg gcgtgagtct
     6601 cttcacactg tgtctgaaaa gccgtcacca gcggcgctaa tgacgcttct ggtagaggcc
     6661 actgagtttc ccagccgtca ctggtgagta ccgcaaccag aggtgcggct tccgcactct
     6721 ctggagcgta ataaaacagc gcgcccaaca cgcgcgccgc gacagaaaaa ttatcttgct
     6781 gtgaaaaatg ggtcattcct aacatcctga aatgtgcggg ttgccccgca ccaatttgtt
     6841 aacctgcaat agccattcct actgtcatgt gcaggccata aaagagcaca cgcccgatca
     6901 tctcaccaac aagtatgagg attagcccca gaacaagtcc ggcaacgtga ggttcacggc
     6961 gacgaatgag cggacaaagc cagcatccaa gacctgcgca taacaatacc acacgccaga
     7021 cctgtaagga ggcgtaatct ggtaccagtg cgctggcttg ttgcacggat gagtgaatag
     7081 acgccagaga caaaccttgc agcacaatca ccccggcaca agcgatcagc gcgagaacgc
     7141 taatgatggc aaatggtgtg gtattaaatg taacgcgggc tgcccgcaga attgcggcag
     7201 caagtattgg accgctcaac aataccgtca ggaagaaagc cagcgtagtg taaccgttat
     7261 gccaggttgg cacagtgtcg atttgataca cgcaggtcat catccagacg aaaatgacgc
     7321 cgagcgccat gctgacaagt aaccagagtt tccccaacgc ttgcggcatt ttaccgatga
     7381 ccgccaccag ccaccatagt ccgccaacgg caaaaaatat ggaaccggca gcaatctcgt
     7441 tactcagacc agaagctccg attcgattca gcgagttaaa cgcccgcagt ggcgatccca
     7501 ggtgcatgac agaggccata aacccgatgc ccatcaacag ccagaggaaa aacatgccac
     7561 ggacaatacg ctgacggtct gcgtcatttt tggcggcgaa ccagccaatt ccgctaacaa
     7621 tcagcgcgcc tactacgcat tgacccaata cggtaaagat aaccagtggc cactcatgcc
     7681 atccatttcc cattttacac ttcctccgga ttagccagat aaccagtggt atcgctggtc
     7741 gggcggctgt tggcgttagg tttgataacg atattgggtt ttgtgaaatg cgcgcgcggc
     7801 agtggcgcga cggcagccag agtgccgtgc ttctggcgca gctcttcaat tggaccgaac
     7861 tcaagtgcac gcagcgggca ggattcgaca catatgggtt gtttcccctc ggcgacgcgc
     7921 gaataacaac catcgcactt cgtcatgtgc cctttttcag cattgtactg tggcgcgccg
     7981 tacggacaag ccatgtggca gtagcggcag ccgatacaaa catcttcatc aaccaccaca
     8041 aagccatctt cacgcttatg cattgcgccg ctggggcaga cttttgtaca tgcgggttca
     8101 tcgcaatggt tacaggagat ggagagataa taagcgaaca cgttctggtg ccagacgcca
     8161 ttatcttcct gccagtcgcc gccagcatat tcataaatac ggcggaaact gacttccggg
     8221 ccaagatctt taaaatcttt gcacgccagt tcgcaggttt tacagccagt acagcggctg
     8281 gaatcgataa aaaatccata ttgtgtggtc acgggctact ccttaaacct tttcgatctg
     8341 gacaagattg ctgtgcgacg ggtttccctt tgccagcggt gaagggcggt gagaggtcag
     8401 aatattgata ctgccgccgt gatcgacccg gtcaccaaac atatccgctt taagccaagc
     8461 accttgcccg atggcggtaa cgccaggcag aatacgcgga gtcacttttg cggcaatcag
     8521 catttctcca ttattgttaa atacccgcac ggtatcgcca tgacggatac cgcgtgcctg
     8581 agcatcaatg gggttgatcc acacctcttg tgggcaggcc tgctgtaaca catcaatatt
     8641 gccgtagctg gagtgggtac gcgctttgta atggaagccc gttaactgca gtggataggt
     8701 tttccgcagg ggatcgtccc agccatcaaa acctggggta tacgcaggaa ggggatgaat
     8761 aacttcatct tttttcaatt cccaggtatc cgcaatcttc gccagtcgtt cagaataaat
     8821 ttcgattttc cccgaaggtg ttttcaacgg gtttgcctgt ggatcttcac ggaatgcgcg
     8881 gaaagcgacg tagtgttctt ccgggcattt tttcttaaag atcccggtcg ttttcatctc
     8941 ctcgtagtcg ggcatctcag ggttacgttc cttcgttttc gcatggagat atttgatcca
     9001 ttcatgctga ctgcgacctt cagtaaaggt ttgataaacg tctggtccta agcgtttggc
     9061 gacttcactc agcatccagt agatgggttt gcgttcaaat tttgctgagg ttgcgggttg
     9121 ggcgaggatc acatagccca tattccctgc agattcatga gagataaggt cttcttgctc
     9181 tgttggcatc aggtcgggca acaggatatc gcaatactta gccgaggccg tcatgaagtg
     9241 gtcaatgcca acaatcatct cgcacttgct gtcatcctga agcacctcat gggtgtgatt
     9301 gatgtcgcca tgttgattga tcaatgtgtt actggcgtag caccataaaa acttgatggg
     9361 gacatccagt ttttcttttc cacgaacacc atcacgg
//
LOCUS       DAEEKQ010000152         9363 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.154, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000152 DAEEKQ010000000
VERSION     DAEEKQ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.154"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            223..2199
                     /locus_tag="KLK57_003388"
                     /pseudo
     CDS             223..2199
                     /locus_tag="KLK57_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311934.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            2192..3328
                     /locus_tag="KLK57_003389"
     CDS             2192..3328
                     /locus_tag="KLK57_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HBE0893674.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDMLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            3325..5325
                     /locus_tag="KLK57_003390"
     CDS             3325..5325
                     /locus_tag="KLK57_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HBE0893675.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     DALIATFSDGVRTQLANGQALKEAQCSCGANGMCRHRVMLVLSYQRLCATTQSTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDMRFYSRSS
                     IRFARCDCIESTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARRDSIGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVEGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            5447..5908
                     /locus_tag="KLK57_003391"
     CDS             5447..5908
                     /locus_tag="KLK57_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HBE0893676.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(5949..6419)
                     /locus_tag="KLK57_003392"
     CDS             complement(5949..6419)
                     /locus_tag="KLK57_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HBE0893677.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="KLK57_003393"
     CDS             complement(6466..7185)
                     /gene="yehT"
                     /locus_tag="KLK57_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HBE0893678.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="KLK57_003394"
     CDS             complement(7182..8867)
                     /gene="btsS"
                     /locus_tag="KLK57_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HBE0893679.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISSP
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            9089..9154
                     /locus_tag="KLK57_003395"
                     /pseudo
     CDS             9089..9154
                     /locus_tag="KLK57_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
BASE COUNT         2273 a         2483 c         2401 g         2206 t
ORIGIN      
        1 cggccacgat acgtattgat tgaaggcccg gctgatttta atgaccgggt agacgaactg
       61 tttttagccc accagcttcc ggtagctatt tacagttatt gccagtatca ggacggtgca
      121 gcccccgggc gtggtgcctg gacgccattt gctgaatttt cgccggagtg gcaggcgcta
      181 caagccgcac gtcgtattca ggcacaaact tacttcatcg atttgccttg ctgggcgcag
      241 agtgaagaag tggacgattc gcctgatacg caagaggaaa gccaggcctt gctgctgcgt
      301 gccacccgca tggataacag cgacaccctg tgggatcact tgttcgaaga tgaaagccag
      361 caaactgcat taccctctgc actggcgcac tattttgccc aactgcgggg cgattccccc
      421 ggcgatgcac tcaatcgtca gcgcgaagcc tttatggccc gctggattgg atgggcgatg
      481 cagcaaaaca atggcgacgt gttagtcgtc tgcggtggct ggcacgctcc tgcactggca
      541 aaaatgtgac gcgaatgccc gcaggacatt aacacgccgg aattgccctc gctggcagat
      601 gccgttacag gttgttatct caccccctac agtgaaaagc gccttgatgt gctggcagga
      661 tacctttccg gtatgcctgc cccggtctgg caaagctggt actggcagtg gggcttacag
      721 caggcgggag aacaactgct aaaaacggtt cttacccgtt tgcgccagca caaattgcct
      781 gcttcgaccg cggatatggc tgccgctcat ctgcatgcga tggcgctggc acagttgcgc
      841 ggacataaac taccgttacg cactgactgg ctggatgcca tagcaggctc gctgattaaa
      901 gaagccctga acgcgccgtt gccgtggagc tatcgcggag ttattcatcc cgacaccgat
      961 ccgattctgc taacgttgat agacacatta gcgggtgacg gattcggtaa acttgcccct
     1021 tctacgccac aaccgcctct gccaaaagat gtcacctgcg aactggaacg taccgcaatc
     1081 tctcttccgg cggagcttac cttaaatcgc tttaacccca atgggctagc gcaaagtcag
     1141 gtgttacatc ggctggcaat actggagatc ccagggattg tacgccagca gggaagcaca
     1201 ctgacacttg caggcaacgg tgaagaacgc tggaaattaa cccgcccgct tagccagcat
     1261 gcggcattga ttgaggccgc ctgctttggt gccacactcc aggaagccgc acgccataaa
     1321 ttagaagccg atatgctgga cgcgggtgga atcggcagta tcaccacatg tcttagccag
     1381 gcggcgttag cgggtctggc gtccttcagt caacaattac tggagcaact cacattatta
     1441 atcgcccagg aaaatcaatt tgccgaaatg ggccaggcgc tggaagtgct atatgcctta
     1501 tggcggctgg atgaaattag cggtatgcaa ggcgcgcaga tattacaaac gacgttatgc
     1561 gcggctatcg atcgcacgct gtggctgtgt gaatctaacg gcagaccaga tgaaaaggag
     1621 tttcacgctc acctgcatag ctggcaggcg ctttgccata tcctgcgcga tctacatagc
     1681 ggcgttaatt tacccggcgt ttttctttct gcggcggtag ccttactgga gcgccgcagt
     1741 caggcgattc atgtcccggc gctggatcgc ggcgcggctc ttggcgcact gatgcgtctg
     1801 gaacatccca acgccagtgc cgaagcggcg ctgacgatgc tggcgcagtt atccccggca
     1861 caatccggcg aggcgctgca cggtttgctg gcgctcgccc gtcatcaact ggcctgccaa
     1921 ccagcattta tcgccggttt cagtagtcat ttaaatcaac tgagtgacga cgattttatt
     1981 aacgccctgc ccgatttgcg cgcggcgatg gcctggctac cgccacgaga acgcgggaca
     2041 ctggcgcatc aggtgcttga gcattaccag cttgcgcagc ttcctgtttc agcgctgcaa
     2101 atgccgttgc attgtcctcc gcaagccatt gcacatcatc aacaactcga acagcaggca
     2161 ctggcatcgc tgcaaaactg gggagttttc catgtctgaa ctgaacgatc ttctgaccac
     2221 ccgtgagcta caacgctggc gattaattct tggcgaagcg gcagaaacga cgctttgtgg
     2281 gctggatgac aacgcccggc agatagacca cgcgctggaa tggctgtatg ggcgcgatcc
     2341 tgaacggctc cagcgtggtg aacgctccgg tggattaggt ggctcaaatc tcaccactcc
     2401 tgagtggatc aacagtattc acacgctgtt tccgcaacag gtgattgagc ggctggaaag
     2461 cgatgccgta ctgcgctacg gcattgaaga tgtggtgacg aatctcgaca tgctggaacg
     2521 tatgcagcct tctgaaagcc tgctacgcgc cgttttgcac accaaacatc tgatgaatcc
     2581 cgaagtactg gctgccgccc gccggatagt gcgccaggtt gttgaagaaa ttatggctcg
     2641 actggcaaag gaagttcgtc aggctttttc tggtgtccgc gatcgccgtc gccgctcatt
     2701 tattccactg gcgcgaaact ttgatttcaa aagtactctg cgcgctaacc tgcaacactg
     2761 gcacccgcaa cacggcaagt tgtatatcga atccccccgc tttaacagcc gcattaaacg
     2821 ccaaagcgaa caatggcaac tggtcttact ggttgatcaa agcggatcga tggtggactc
     2881 ggtgatccac tctgcggtga tggcggcctg tttgtggcag ttacccggca ttcgtaccca
     2941 tctggtggcg tttgacacaa gcgtggttga tctcacggca gacgttgccg atccggtaga
     3001 gttattaatg aaagtacagt tgggcggcgg gaccaatatc gccagtgccg tggagtatgg
     3061 tcggcaactt attgaacaac cagcaaaaag cgtcattatc ctcgtgagcg atttttacga
     3121 agggggttca tcatcattgc tgacgcatca ggtgaaaaag tgtgtccaga gcggcatcaa
     3181 agtgctggga ctggctgcgc tcgatagcac cgcaacacct tgctatgacc gcgatacggc
     3241 ccaggcgctg gttaatgtcg gcgcacaaat agccgccatg acgccgggcg agctggcatc
     3301 atggcttgcg gagaatcttc agtcatgaat tcactacgtc cggaattatt agaactgaca
     3361 ccgcaggccc tgacggcgtt aagcaatgcc ggttttgtta agcgcagtct taaggaactg
     3421 gaaaatggca acgtcccgga gatcagccat gagaacgacg ctttaatcgc caccttcagt
     3481 gacggtgtcc gtacccagct ggcgaacggc caggcactga aagaggctca gtgcagttgc
     3541 ggggccaacg gtatgtgccg tcatcgcgtg atgctggtgt taagttatca acgactttgt
     3601 gccaccactc agtctacgga aaaagaagaa gagtgggatc cggcaatctg gctggaagaa
     3661 ctggctaccc ttcccgatgc tacccgcaaa cgcgcacagg cgctggtcgc taaaggcatc
     3721 accattgagt tgttctgtgc gccgggtgaa attccctctg cccgcttacc gatgagcgat
     3781 atgcgttttt attcccgcag cagtattcgt ttcgcccgtt gtgattgtat tgaaagcaca
     3841 ctttgcgaac atgtcgtact ggcagtacag gcctttgtcc aggcaaaagc gcagcaagcg
     3901 gaattgactc atttaatctg gcagatgcgc agcgaacacg tcacatcatc tgacgatccg
     3961 tttgccagcg aagaaggcaa aacgtgtcgt caatatgttc aacaactaag ccaggcatta
     4021 tggctgagcg gcatcagcca gccgcttatt cactacgagg ccgctttcag ccgcgcgcag
     4081 caagcagcgg aacgctgcag ctggcgatgg gtgagtgaat cgctacggca actgcgcgcc
     4141 agcgttgatg ccttccacgc ccgcgccagc cactatcatg ccggagaatg cttacgtcag
     4201 cttgcggcat taaacagtcg attaaattgc gcacaagaga tggcccggcg cgacagtatt
     4261 ggtgaagttc ctcctgtgcc gtggcgcacg gtcgttggct ctggcattgc cggagaagca
     4321 aagcttgatc atctgcggct ggtgtcttta ggtatgcgtt gctggcagga tattgagcat
     4381 tatggtttac gcatctggtt taccgatccc gacaccggca gtattttgca cctttcgcgc
     4441 agttggccgc gaagtgaaca ggaaaactca ccggcagcta cgcgtcggct gtttagtttt
     4501 caggctggcg cactagcggg cgggcaaatt gtttcacaag cagcaaaacg cagtgccgat
     4561 ggcgagctgc tgttagctac ccgcaaccgc ttaagcagcg ttgtgccgct gtcgcctgat
     4621 gcctggcaaa tgttgagcgc gccgttacgc cagccgggca ttgtggcttt gcgggaatat
     4681 ttacgccagc gtccccccgc ctgcatacgg cctcttaatc aggtcgataa cttatttatt
     4741 ctgccggtcg ctgagtgtat ttcgctcggt tgggatagca gccgccagac gctggatgcg
     4801 caggtaatca gcggcgaagg tgaagataat ctcctgacgt tatcccttcc ggcatctgcc
     4861 tgttctcctt ttgccgttga acgcatggcg gcgcttttgc aacaaacaga cgaccccgtg
     4921 agtctggttt ctggctttgt cagctttgta gaggggcaat tgacactgga accacgggtg
     4981 atgatgacaa aaacccgcgc ctgggcgctg gacgcagaaa ctgcgcctgt ggcaccgcta
     5041 ccttctgcca gcgttttgcc tgtgccgtct accgctcatc agttgctgat gcgctgccag
     5101 gcgttactta ttcaactgct ccataacggc tggcgctatc aggaacagag cgctattagt
     5161 caggcagagt tgctggcgaa tgacctctcc gcggtcggtt tttatcggct ggcacatgtg
     5221 ttgggacaat ttcgtaatac agaaagcgag gcacgggtag aagcaatgaa taacggtgtt
     5281 ttactttgcg aacaattatt ccccatgctt cagcaacaag gatgaaatag tgcttactaa
     5341 gagttctact ccagttccgg actgctcacg ccacggtatt aggcatatcc tatatagccc
     5401 ctggtgagag tcaccagttc cttgattaaa taaaatggag ttttacatga aggctttcaa
     5461 taagctgttt tccctcgttg ttgcatctgt tctggttttc tctcttgctg gctgcggtga
     5521 caaagaagaa tcgaagaaat tcagcgccaa tctgaacggc actgaaattg ccattaccta
     5581 tgtctacaaa ggtgacaagg tgcttaagca atcttctgaa accaaaattc aatttgcctc
     5641 cattggtgca accaccaaag aagatgctgc caagacactt gagccgttaa gcgccaaata
     5701 caaaaacatc gcgggtgttg aagaaaaatt aacctatacc gatacctacg cgcaggaaaa
     5761 cgtgactatc gatatggaaa aagtggattt taaagccctg cagggtattt caggaatcaa
     5821 cgtttctgct gaagatgcca aaaaaggtat cactatggcg caaatggaac tggtgatgaa
     5881 agccgctggt tttaaagaag tgaaataatc tgtcggcggc catgtttcgc atggccgcca
     5941 tacccgtctt agcttttctt cacatgctgg cgcgctgcca gtccacgcag aaaataacgt
     6001 aaaaattgat cgccgcattc gcggaagttt ttatgatccg gtgcgcgcat catcgctgta
     6061 atttccggca tcgaaacgcg gaactgctgt tcggtgagga tcgccagaat gtcatcggtt
     6121 ttcagcgaaa acgcgatgcg taattttttc agcacgatgt tgttattaat gcgacgttcc
     6181 ggctccagtg ccggagcaga ctcatccttg ccgcgttttt cataaatcag gccattgagg
     6241 aatgacgaca aaacaatgtc cggacaacgc tgaaaaccct cttcgtcttc tttacgtagc
     6301 cagacggcga tctgttccgc ggtggcttcg acattaccca gcgccagaat acgcaccagg
     6361 tcattattat tggctttcaa aatgtagcgc acgctgcgca gaatatcgtt acttagcatg
     6421 aggccttcag gtgttaatga ggcaaaaagc cattttagca gtcttttaca ggccaatcgc
     6481 ctcttttaag cttttcagat aacggcggct gaccggcacg gttaagccat tacgcaaaat
     6541 caactcggcc tggccgttat cttccagacg aatctcctgt aaatgcgcga ggttaaccag
     6601 atactgacga tggcagcgca gtagtggtgt acgactttcc agggtacgta atgtcaattc
     6661 ggtaaagccc tctttccctt cgtggctggt aacgtagaca ccgctcatcc gactgctgac
     6721 aaatgccaca tctttcattt gcagcaaata aatccgacta tgccccgtac aagggataaa
     6781 tttcagcgcc tgttgatttt ccggtaacag cgaaacatcc tgcttgctgc gctcctgacg
     6841 caatcgcgcc agcgttttct ccagtcgcgc ttcatcaatt ggcttcagca gataatcaaa
     6901 ggcatgttct tcaaaggctt taatagcgta ttcgtcaaac gcagtgagaa aaacaatata
     6961 cgggcgatgt tccgggtcaa gcatccccac catttccaga ccactgatgc gcggcatctg
     7021 gatatcgaga aacagcacat ccgggcgcag tttatgcacc gcgccgatcc cttccacggc
     7081 gtttgaacac tctccaacga tttcaatatc gctctgctcc tgcaaaaata cacgcaggtt
     7141 ctcccgtgct aacggttcat catcgacaat taagacttta atcatgcctc gtccctccat
     7201 ggtagtcgta acgttattcg ggtgtaacta tcaggctcac aggcgacgct tattccatag
     7261 tcatcgccaa accgttcacg taaacgctta tccaccagat tcatccccag cccactggca
     7321 ttggttaccg gttgatacaa accggcattg tcttcgatct ccagcatcaa atgttgcccc
     7381 tcacgtcggg cgctgattgc cactcgccct gtatccagca gttgtgatgt cccatgttta
     7441 atggcgtttt ccactatcgg ttgcagggta aacgcgggca attgctgctg ggataattct
     7501 tgcggaatag caatgttgac ctgcaaccgc gactggaagc gcgccttttc aatttgcaga
     7561 taagcgttca catgttcaat ttcgtcggcg agagtaacaa actccgaagg ccgctttaag
     7621 tttttgcgga aaaaagtgga aagatactgc accagctggc tggcctgttc gctgtcgcgg
     7681 cggatcaccg ctttaatggt gttaagcgca ttaaacaaaa aatgaggatt cacctgggcg
     7741 tgaagcagtt tgatttctga ctgggtgagc atcgcttttt gccgctcata ttgcccggca
     7801 agaatctgcg ccgaaagcag ttgcgcaatc ccctcgccca gcgtgcggtt tattgaactg
     7861 aataaacggt ttttggcttc atacaatttg atggtgccca tcacgcgctg attttcgcca
     7921 cgcaacggaa ttaccagcgt cgaccccagt ttgcattgcg gatgcaaaga gcaacgataa
     7981 ggtacttcgt tgccatcagc gtagaccact tcaccggttt caatcgcttt taaggtgtaa
     8041 ggcgaagaaa tcggtttgcc gggtaaatgg tggtcgtcac caattccggt aaaggccagc
     8101 aatttctctc gatcggtaat cgcgactgca ccaatatcca gctcctgata cagcacctga
     8161 gccactttca tgctgttcac ttcgttaaac ccctgtcgca aaatgccttc cgtcgaggct
     8221 gccactttca gcgcagtggc agaaaaagcc gaagtgtatt tttcaaacat cgcgcgttta
     8281 tcgagcaata tacgcataaa cagcgccgcg ccgacggtat tggtgaccat cattggcgca
     8341 gcaatattac tcaccagacg caccgcatct tcataaggtc gggcgatcgc caggatgatc
     8401 agcatttgca ccatttcagc gacgaacgtg acggcaccgg cggtaatggg gttaaagact
     8461 ttatcagtgc gcccgcgacg gatcaggatg ctgtgtacca ggccgccgag caatccttca
     8521 acgatggttg agatcatgca acttagcgcg gtcatgcccc ccatcgaata tcgatgtaag
     8581 ccgccggtca gaccaaccag cccaccgacg accggaccgc cgagtaagcc gcccattacc
     8641 gcgcctatcg cacgggtatt ggcaatagaa tcgtcaatgt gcaacccaaa ccaggtgccc
     8701 atgatgcaga agatggaaaa gacgatgtag cagagaaatt tatgcggcag acgaaccgtg
     8761 acctgcatta acggtatgaa taatggcgtt ttactcatta accacgcaat gactaaaaaa
     8821 acgcacatct gctgaagcag cagcaacacc agattaaaat cgtacatacc cgcaaaccac
     8881 acttcccttt aaaacgcgta acatacattg cctgcgttta actttctttg aactcttgca
     8941 gaaaaatgag aattcgtgag tacgatcact caaaatcgcc tggcaaaaat aaaatcaccc
     9001 tatagatgca caaaaaacgg gcaaaactac ctggttcgca aaactgcgtc taaagttaaa
     9061 ccgggacctc gcgagcaagg gtgagacgat ggcgctttac acaattggtg aagtggcgtt
     9121 gctttgtgat attaaccctg tcacgttacg cgcgtgacgc aacattttta aaaatcgacg
     9181 aagatcgtcc aggagcgccg tttttcaagg gttggataga tgattcgttt accatgtcga
     9241 cgaacttcga cgaatttcgc cgataaaaag tgtcccatac atgccccatt gatgccccat
     9301 aaacaatatc aggaattgac actggttatc catacagata aaaataacac ctgtacataa
     9361 cca
//
LOCUS       DAEEKQ010000153         9188 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.155, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000153 DAEEKQ010000000
VERSION     DAEEKQ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9188
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.155"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            171..1769
                     /gene="rtcR"
                     /locus_tag="KLK57_003396"
                     /pseudo
     CDS             171..1769
                     /gene="rtcR"
                     /locus_tag="KLK57_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
     gene            complement(1751..2509)
                     /locus_tag="KLK57_003397"
     CDS             complement(1751..2509)
                     /locus_tag="KLK57_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HBE0893680.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="KLK57_003398"
     CDS             complement(2526..3356)
                     /gene="glpG"
                     /locus_tag="KLK57_003398"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HBE0893681.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPALKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="KLK57_003399"
     CDS             complement(3401..3727)
                     /gene="glpE"
                     /locus_tag="KLK57_003399"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HBE0893682.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            3917..5422
                     /gene="glpD"
                     /locus_tag="KLK57_003400"
     CDS             3917..5422
                     /gene="glpD"
                     /locus_tag="KLK57_003400"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HBE0893683.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDDRM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGAISDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(5474..6079)
                     /locus_tag="KLK57_003401"
     CDS             complement(5474..6079)
                     /locus_tag="KLK57_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893684.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWPPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(6082..6849)
                     /locus_tag="KLK57_003402"
     CDS             complement(6082..6849)
                     /locus_tag="KLK57_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893685.1"
                     /translation="MQPSPAMQALIEQIYHTFRRYRVPQKFVVCCEYCLSQQEQKALR
                     STSLRAIPYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGHFDNIHVVFSLRRIN
                     LANKENWRADERAILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNAITTQINAWARNNHPLLKERARQAIEN
                     PLKQPEPMTEYQVWEDDWIIDECLCAMYDASSESPGK"
     gene            complement(6852..7562)
                     /locus_tag="KLK57_003403"
     CDS             complement(6852..7562)
                     /locus_tag="KLK57_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893686.1"
                     /translation="MELVNTLFASLAGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYDVTLNIGLTSSKVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDVTVQFTAMLNPDERVIIAKRPLKKCDSWIEDVYIFQVFKDAW
                     KFHNNNSLRGFRDKQAELKLYTRHYSVENCAEESCRDCNSCIRPSFSLSRSALIRLNA
                     ANARFVYQPFTRDQRARG"
     gene            complement(7578..9083)
                     /locus_tag="KLK57_003404"
     CDS             complement(7578..9083)
                     /locus_tag="KLK57_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001472437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HBE0893687.1"
                     /translation="MQNRKWILSSLVMTFFGIPILTQFLAAVVAMLGVGLAGIIEVCN
                     ILITPTSYLLLNIFMLALGALMLFFSGRVWAGDSAPEKREIAVWRQCLFLVPGLLILV
                     GWIIALHLADYQFHQMGSGWLADLMLPWLGVLLVSVVGGEYWWIVIIPVGAHISFSLG
                     YGRPTRHPLTGTSGLRCRNSLLFILLMLGFVAGYQGYLYKQLNPGVGVRENIDTWAWR
                     PDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVIPEDFHTREYLES
                     SRTPDAYNRIVKGDADIIFVAQPSGGQKKRAEESGVTLLYTPFAREAFVFIVNADNPV
                     NSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISPQ
                     ETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENIR
                     NGKYPYIVDAFMVTRENTTSETQKLLEWFLTPQGQSLVEDVGYVPMYKTLP"
BASE COUNT         2372 a         2359 c         2262 g         2195 t
ORIGIN      
        1 ttgtttgctg tcgtgataat tgcaaccgtc gtgccagaaa attaaataag cagattaatt
       61 ttttaattca ttgtttttaa ataggttaaa tctttacgtc cgtaaacgga gattttccac
      121 aaaaccaatt caccgataat gaaatatcgt cttttataag gatatctaag atgcgtaaaa
      181 cagtggcttt tggctttgtc ggtaccgtgc tggattatgc cggacgcggc agtcagcgct
      241 ggtcgaaatg gcgtccgacg ctttgtttat gccagcaaga atcgttagtc atcgatcggc
      301 tggaattgtt gcacgacact cgctcgcgct cgctattcga aacgcttaaa cgcgatatcg
      361 ccagcgtttc gccagaaaca gaagtcgtgg gcgttgagat tgaactgcat aacccgtggg
      421 atttcgaaga ggtttacgcc tgcctgcatg atttcgcccg tggttacgag tttcagccag
      481 aaaaagaaga ctatttaatt cacatcacca ccggcaccca cgtcgcgcag atttgctggt
      541 ttctgctggc ggaagcgcgt tacctgcccg cccggctgat acaatcttca cctccgcgca
      601 aaaaagaaca gccgcgcggc gcaggtgaag tgacgattat cgatctcgat ttaagccgtt
      661 ataacgccat cgccagccgc tttgccgagg aacgccagca aacgcttgat tttcttaagt
      721 ccggcattgc cacgcgtaac ccccacttca accgcatgat tgagcagatc gaaaaagtgg
      781 cgatcaaatc ccgcgcgccg attctgctta acggtccaac cggcgcgggc aagtcatttc
      841 tggcgcgacg catcttcgag ttaaaacagg cgcggcatca gtttagcggc gcgtttgtgg
      901 aagtgaactg cgccaccctg cgcggcgata ccgccatgtc gacgctgttt ggtcatgtaa
      961 aaggcgcgtt taccggggcg cggtaatctc gtgaaggttt attacgcagc gccaacggcg
     1021 gaatgttgtt tcttgatgag attggcgaac tgggcgcaga cgaacaggca atgctgctaa
     1081 aagccattga agagaaaacc ttttacccgt ttggcagcga tcgccaggtg agcagtgatt
     1141 ttcagcttat cgccggaacg gtgcgcgatt tgcgccagct ggttgccgaa ggcaaatttc
     1201 gcgaagatct gtacgcgcgg atcaatctct ggaccttcac cctgccgggt ctacgccagc
     1261 gccaggaaga tattgaaccg aaccttgatt atgaagtgga gcgccacgcc acactcactg
     1321 gcgacagcgt gcgttttaac accgaagcgc ggcgcgcctg gttggctttt gcgacctctc
     1381 cccaggcaac atggcgcggt aactttcgcg aactttctgc cagcgtcacg cggatggcca
     1441 cctttgccac tagcggacgc atcactctgg acgtggttga agatgagata aaccgtctgc
     1501 gctataactg gcaggagagc cgcccctccg cgcttacggc gttgctgggc gcagaggcag
     1561 aaaacatcaa tctcttcgac cgtatgcaac tggaacacgt tatcgctatc tgccgctagg
     1621 caaagtcgct ttctgcagcc ggacgacagc tttttgatgt ttcgcgccag ggcaaagcca
     1681 gcgtcaatga cgcggatcgg ctacgcaaat acctggcgcg ttttggtctg acgtgggaag
     1741 ccgtgcagga tcagcacagc tccagttgaa tatgatggtc cgtcagcacc tgcattacgc
     1801 ttactggcgg cggggcgtcg gtgtagacgg catctaccat gctgatgctg cccatattga
     1861 ccatcgcgtt acggccaaat ttcgagtgat cgacaaccag cataacgtgg cgcgagttct
     1921 caataatggc gcgcttggtg cgaacttcgt ggtaatcgaa ctccagcagc gagccgtcgc
     1981 tatcgatgcc gcttatcccc agaatgccga aatcaaggcg gaactgggag ataaaatcga
     2041 gcgtcgcttc gccaatgatc ccgccatcgc ggctgcgtaa ttcgccaccg gcgagaataa
     2101 tgcggaaatc ttcttttacc atcaacgtgt tagcaacatt gagattgttg gtgacaatac
     2161 gcaaattgct gtgattaagc agcgcgtgcg ccaccgcttc cggcgtggtg ccgatatcga
     2221 taaacagtgt cgagccgttg gggatttgct ccgccacttt gcgggcaatc ctctcttttt
     2281 cttcggtctg ggtggctttg cgatcgtgcc acggcgtgtt aaccgaactg gaaggcagcg
     2341 ccgcaccgcc atggtgacgc aggatcaggt tttgctccgc cagctcattg aggtcgcggc
     2401 gaatagtctg cgggctgacg gagaaatgct ctaccagctc ttcagtactg acataaccct
     2461 gctgtttaac cagttcgata ataccgttgt gacgttgtgt ttgtttcatt tataaatccc
     2521 tggaattatt ttcgttttcg cgcattgagc gaatcaacaa aagccatcgc taaacccacg
     2581 gctaacccgg cgatgtgtgc tccgttcgcc atcgacatcc caaacaaatc aaaccatccg
     2641 gcgacaatcc agatcagcgc aaagataatt aacccacgtt gcaggtaaat gccactttgc
     2701 ggatcgcgtt caccacgtag ccagacgtag cccatcagcg catacaccac gccagaaagc
     2761 ccgccaaacc acggcccgct gaatttttgc tgcacatagc cgcttaacag ggcgctaatg
     2821 agagtaataa caattagctt accgctaccg aggcgttttt ccaccgcacc gccgagatac
     2881 caccaccaga gcaggttaaa gaggatatgc atcagcgaga agtgcattaa cgcgtgggtg
     2941 aagtaacgcc agaactcaaa tttcagtgct ggatcgaatg gccaggccag ccataacatc
     3001 acttcctgat cgccgagaat ttgcatggca ataaacacca ccacgcaggc gatcatcatc
     3061 acccaggtca ccggacctgc gcgttcacgc aaggcggcaa agaaaggata acggcgataa
     3121 tgcaggccac tgccggtatg gcctgcctgc cagctggccg ccagataacg cggatctgcc
     3181 gggttttcga gaaaacgcgc cagctccgcc cgtacgcgct cggcctggga ctcatccgcc
     3241 agccagacat cgctttggtt atgttgttga atcgtgagga taacaccctg cgtcgccatg
     3301 taatcaacaa acgcctgcgc cacgcggggg ttagcaaaag aggtaatcat caacatcgtt
     3361 gctgtcgctt attccacaca aaaggggaca gtataaagcg ttacgcgccg tacgccacct
     3421 ctgcgggaaa ctgacgttgc caggcttcaa agccgccgtc aatgctatag accacatcgt
     3481 agccctgttg cagcagatac tgcgccgcgc ctttgctgct attgccgtga taacacatca
     3541 ccatcaccgg agtgtcaaag tcgttatcac gcataaacgc gcccagcgtg tcgttggtta
     3601 aatggaaagc ctgcaccgca tgccccatag cgaaactctg tggatcgcga atatcgacca
     3661 gcaccgcctc tttttcctgc aacttctggt gcgcgtcggc aacgttaata cattcgaact
     3721 gatccatgcg tctctctttc tttacaaaca agtgggcaaa tttaccgcac agtttacgtc
     3781 gaagcggcag ataaacgcca taatgttata catatcactc taaaatgttt tttcaatgtt
     3841 acctaaagcg cgattctttg ctaatatgtt cgataacgaa catttatgag ctttaacgaa
     3901 agtgaatgag ggcagcatgg aaaccaaaga tctgattgtg ataggtggcg gcatcaatgg
     3961 tgctggtatc gcggcagacg ccgctggacg cggtttatcc gtgctgatgc tggaggcgca
     4021 ggatctcgct tgcgcgacct cttccgccag ttcaaaactc attcacggtg gcctgcgcta
     4081 ccttgagcac tatgaattcc gcctggtcag cgaggcgctg gctgaacgtg aagtgctgct
     4141 gaaaatggct ccgcatatcg ccttcccgat gcgttttcgc ctgccacatc gtccgcatct
     4201 gcgcccggcg tggatgattc gcattggtct gtttatgtac gatcatctgg gtaaacgcac
     4261 cagcctgccg ggatcaactg gtttgcgttt tggcgcaaat tcagtgttaa aaccggaaat
     4321 taagcgcgga ttcgaatatt ctgactgttg ggtagacgac gcccgtctgg tactcgccaa
     4381 cgcccagatg gtggtgcgta aaggcggcga agtgcttact cggactcgcg ccacctctgc
     4441 tcgccgcgaa aacggcctgt ggattgtgga agcggaagat atcgataccg gcaaaaaata
     4501 tagctggcaa gcgcgcggct tggttaacgc caccggcccg tgggtgaaac agttcttcga
     4561 cgacaggatg catctgcctt cgccttatgg cattcgcctg atcaaaggca gccatattgt
     4621 ggtgccgcgc gtgcataccc aaaagcaggc ctacattctg caaaacgaag ataaacgcat
     4681 tgtgttcgtg atcccgtgga tggacgagtt ttccatcatc ggcaccaccg atgtggaata
     4741 caaaggcgat ccgaaagcgg tgaagattga agagagtgaa atcaattacc tgctgaaagt
     4801 gtataacacg cactttaaaa agcagttaag ccgtgacgat atcgtctgga cctactccgg
     4861 tgtgcgtccg ctgtgtgatg atgagtccga ctcgccgcag gctattaccc gtgattacac
     4921 ccttgatatc catgatgaaa acggcaaagc accgctgctg tcggtattcg gcggtaagtt
     4981 gaccacctac cgcaaactgg cggaacatgc gctggaaaaa ctgacgccgt attatcaggg
     5041 cattggcccg gcatggacga aagagagcgt gctgccgggt ggtgccattg aaggcgaccg
     5101 cgacgattac gcagctcgtc tgcgccgtcg ctatccattc ctgactgaat cgctggcgcg
     5161 tcattacgca cgcacttacg gcagcaacag cgagctgctg ctcggcaatg cgggggcgat
     5221 aagcgacctc ggggaggatt tcggtcatga gttctacgaa gcggaactga aatacctggt
     5281 ggatcacgaa tgggtccgcc gcaccgacga tgccctttgg cgtcgcacaa aacaaggcat
     5341 gtggctgaat gcggatcaac aatctcgtgt gagtcagtgg ctggtggaat atacgcagca
     5401 gaagttgtcg ctggcgtcgt aaggatgatg cccggttgaa gtgcaaccgg gctatttgga
     5461 ttccttagac tattcattgt aaataattga ataacggccc ctgagctaaa atcgccaaac
     5521 ttaagcacgt tacccaatcg ccccacttct tcctctggtt tctatttttt ctgcaccaat
     5581 ggcaataata tcccaatgca aaaccaagct gtgcactaat cgggccgaca aaatcagtcc
     5641 aaggctgcgg ccaaaaaggg gaaaaattca ggggaggcca gacaaacgtc agaaagaaat
     5701 ttcggatatt ttccagcaac gctgatttct ctggagaaat ataaagaatg aaattcgaga
     5761 gaattaacca gaaatagacc ggcgaaaaga ccagccagca atgcagtctg ttttttaatt
     5821 caggaggcgt tctgcgggag aattgccaac cgatgccagc cattacactg aaaaaaagtg
     5881 catattcaac cattaactca agttgtaatt cctgaagaga aatacgcaca accaggagcg
     5941 gaaaggagaa tattatttct gccagaaaag ttaacgtgaa tataagcacg gctaagacaa
     6001 taaccgtcgg ccataccaat aatggcacga taacaaaacc gagaattaca gggggaatat
     6061 aaagccggaa tttaatcatt tttattttcc tggtgattcg gatgatgcgt catacatcgc
     6121 gcataaacac tcatcaatta tccagtcatc ttcccagacc tgatattctg tcattggttc
     6181 gggctgtttt agcgggttct caatcgcttg ccgcgctctt tctttcagta aaggatgatt
     6241 atttctagcc caggcattga tttgggtggt aatggcgtta tcgatatcgt cctggttatc
     6301 tctgatataa tcctcacaat atctgttcaa taaacaggca agagaaacgg tcgaccaaaa
     6361 gccgggaact gaattaatgg catccagcaa tggcgcaaga gcgatatcgg cacggaaaaa
     6421 catttccagc atatactgta attcgacagc ttcatcgccg gatacccaat cagtcatata
     6481 ctggcaggca aatcgctgta aaattgcccg ttcatccgca cgccagtttt ctttgttggc
     6541 aaggttgata cggcgcagag aaaaaactac gtgaatattg tcaaaatgcc cctgtgcaac
     6601 aaattccagt aaacgcggca gaaaatagcg gacctcatcg ctattttgcg gatcaggccc
     6661 tgggctacta ttccaggcat tgattaagga atacgggatc gctcgtaacg atgtactccg
     6721 taatgccttt tgttcctgct gactcaggca atattcacaa caaaccacaa atttctgcgg
     6781 cacccgataa cgacgaaagg tgtgataaat ctgctcaatt aatgcctgca tggcggggct
     6841 gggttgcata gttaccccct cgcccgctga tcgcgtgtaa acggttgata gacaaaacga
     6901 gcattcgccg cgttcaggcg aataagggcg ctacgcgaaa gggaaaagct ggggcgaatg
     6961 caactattgc agtctcgaca gctttcctct gcgcaatttt ccactgagta atgacgcgtg
     7021 tagagtttca gttcggcctg cttatcgcga aatccgcgta aactgttatt gttatgaaat
     7081 ttccaggcat ctttaaagac ctgaaagata tagacatcct caatccagga gtcacatttt
     7141 ttaagcgggc gctttgctat tatcacccgc tcatccggat ttagcatcgc cgtaaattgt
     7201 acggtgacgt catttgtcgt gttcaggtca cattgaacat ctggtacatc actgatatag
     7261 gggtcaaatc caaaatcagc accacaggca caaagacggc taatggtggc ggtatcgagg
     7321 gaaaatcccg gccatacctt gctggaggtt aagccaatat ttaatgtgac atcatatcga
     7381 agcaatccct caagaccgtc agcaccgacg aacttttccc cttcatccaa ggcctggtta
     7441 ataagttgct gaacaatgcc ttcatcccag tatgccgatt tgcagttggc aatcgtaata
     7501 tccacgcctg tgaagggatc agtaccggca aggcttgcaa ataatgtatt aactaattcc
     7561 atttttatta tccagcttta aggtagcgtc ttatacatcg ggacataacc tacatcttcc
     7621 accaaactct gtccctgcgg cgttaaaaac cactccaaca atttttgtgt ctctgacgtc
     7681 gtattttccc gcgttaccat aaaggcatcg acgatatagg gatatttgcc gttgcgaatg
     7741 ttttcggcag tcggtgcaat accgttaatc gctagcagtt ttatattttt atcggcgttc
     7801 atttgtgttg cgtaataacg gaaggtgtaa cctattgcgt tgtttgtatt acggtattcg
     7861 gcaacgactt taatcattcc ctccatcata cttgccactt ctgtttcctg cggcgagatc
     7921 atgcggacat ttttcatgac ctgggattgc atcaccgtct ggctaccaga gtcttccggg
     7981 cgttgccagg tctggatctc ctgatcgtta ccgccaacag tacgccagtt ggtaattgca
     8041 ccactgaaga tgtcacgcac ctgttgttca gtcagggaat taaccggatt atccgcattg
     8101 acgatgaaaa caaacgcttc acgggcaaaa ggcgtgtaca gcaaagtaac gcccgattcc
     8161 tcagcgcgtt ttttctgccc accggaaggt tgcgccacaa aaataatatc agcatcacct
     8221 ttaacaatcc tgttatacgc atcgggggta cgagagctct ccagatattc cctggtgtga
     8281 aaatcttccg gtataacgct taaagcataa aatgcagagg cataaattgg gtacgccgcc
     8341 gtagcaccat cgagtcgcgg ccagttttgc gtaaactgaa tttgcggttt accacgcagt
     8401 ggtgtcagtt gattattgag tttatcgggt cgccaggccc aggtatcaat attttcgcgc
     8461 acaccgacac cgggattgag ctgtttatat aaataaccct gataaccagc gacaaaacca
     8521 agcatgagaa ggataaacag gagggaatta cggcaacgta gcccggatgt accggttaaa
     8581 ggatgtctgg ttggccggcc gtaccccagc gaaaaactga tatgcgcgcc aacgggtata
     8641 atgactatcc accagtactc acctccgact actgagacta acaaaacgcc cagccagggg
     8701 agcataagat cagccaacca acccgatccc atctggtgaa attgataatc tgccagatgc
     8761 agcgcgatta tccagccaac caggattaat aaaccgggta ctaaaaaaag acattgtcgc
     8821 cagacggcta tttctctttt ttctggtgca ctatcgcctg cccatactcg ccccgagaaa
     8881 aatagcatta atgcgcccag cgccagcata aaaatgttga gcagaaggta acttgttggc
     8941 gtgataagga tattacaaac ttcaataata ccggcaagtc cgacacccag catagcaaca
     9001 accgccgcca aaaattgcgt cagtatgggg atgccgaaaa aagtcattac cagcgaggac
     9061 aaaatccatt ttctgttttg cattattctt tccattcttt ttgaatggtg aaattatact
     9121 ccccgagtcc ccttgcccct tctggacact tttccgaaat gatggcggaa aaaaacggga
     9181 ccctttgg
//
LOCUS       DAEEKQ010000154         9072 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.156, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000154 DAEEKQ010000000
VERSION     DAEEKQ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9072
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.156"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(506..1273)
                     /gene="fecE"
                     /locus_tag="KLK57_003405"
     CDS             complement(506..1273)
                     /gene="fecE"
                     /locus_tag="KLK57_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter ATP-binding
                     protein FecE"
                     /protein_id="HBE0893688.1"
                     /translation="MTLRTENLTVNYGTDKVLNDVSLSLPTGKITALIGPNGCGKSTL
                     LNCFSRLLMPQSGTVFLGDNPINMLSSRQLARRLSLLPQHHLTPEGITVQELVSYGRN
                     PWLSLWGRLSAEDNARVNVAMNQTRINHLAVRRLTELSGGQRQRAFLAMVLAQNTPVV
                     LLDEPTTYLDINHQVDLMRLMGELRTQGKTVVAVLHDLNQASRYCDQLVVMANGHVMA
                     QGTPEEVMTPGLLRTVFSVEAEIHPEPVSGRPMCLMR"
     gene            complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="KLK57_003406"
     CDS             complement(1274..2230)
                     /gene="fecD"
                     /locus_tag="KLK57_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter permease
                     subunit FecD"
                     /protein_id="HBE0893689.1"
                     /translation="MKIALVIFITLALAGCALLSLHMGVIPVPWRALLTDWQAGREHY
                     YVLMEYRLPRLLLALFVGAALAVAGVLIQGIVRNPLASPDILGVNHAASLASVGALLL
                     MPSLPVMVLPLLAFAGGMAGLILLKMLAKTHQPMKLALTGVALSACWASLTDYLMLSR
                     PQDVNNALLWLTGSLWGRDWSFVKIAIPLMILFLPLSLSFCRDLDLLALGDARATTLG
                     VSVPYTRFWALLLAVAMTSTGVAACGPISFIGLVVPHMMRSITGGRHRRLLPVSALTG
                     ALLLVVADLLARIIHPPLELPVGVLTAIIGAPWFVWLLVRMR"
     gene            complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="KLK57_003407"
     CDS             complement(2227..3225)
                     /gene="fecC"
                     /locus_tag="KLK57_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dicitrate ABC transporter permease FecC"
                     /protein_id="HBE0893690.1"
                     /translation="MTAIKHPVLLWGLPVAALIIIFWLSLFCYSAIPVSGADATRALL
                     PGHTPTLPEALVQNLRLPRSLVAVLIGASLALAGTLLQTLTHNPMASPSLLGINSGAA
                     LAMALTSALSPTPIAGYSLSFIAACGGGVSWLLVMTAGGGFRHTHDRNKLILAGIALS
                     AFCMGLTRITLLLAEDHAYGIFYWLAGGVSHARWQDVWQLLPVVVTAVPVVLLLANQL
                     NLLNLSDSTAHTLGVNLTRLRLVINMLVLLLVGACVSVAGPVAFIGLLVPHLARFWAG
                     FDQRNVLPVSMLLGATLMLLADVLARALAFPGDLPAGAVLALIGSPCFVWLVRRRG"
     gene            complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="KLK57_003408"
     CDS             complement(3222..4124)
                     /gene="fecB"
                     /locus_tag="KLK57_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418710.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate ABC transporter
                     substrate-binding protein FecB"
                     /protein_id="HBE0893691.1"
                     /translation="MLAFIRFLFAGLLLVISHAFAATVQDEHGTFTLEKTPQRIVVLE
                     LSFADALAAVDVSPIGIADDNDAKRILPEVRAHLKPWQSVGTRAQPSLEAIAALKPDL
                     IIADSSRHAGVYIALQQIASVLLLKSRNETYAENLQSAAIIGEMVGKKREMQARLEQH
                     KERMAQWASQLPKGTRVAFGTSREQQFNLHTQETWTGSVLASLGLNVPAAMAGASMPS
                     IGLEQLLAVNPAWLLVAHYREESIVKRWQQDPLWQMLTAAQKQQVASVDSNTWARMRG
                     IFAAERIAADTVKIFHHQPLTVVK"
     gene            complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="KLK57_003409"
     CDS             complement(4169..6493)
                     /gene="fecA"
                     /locus_tag="KLK57_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+) dicitrate transport protein FecA"
                     /protein_id="HBE0893692.1"
                     /translation="MTPLRVFRKTTPLVNAIRLSLLPLAGLSFSAFAAQVNIAPGSLD
                     KALNQYAAHSGFTLSVDASLTRGKQSNGLHGDYDVESGLQQLLDGSGLQVKPLGNNSW
                     TLEPAPAPKEDALTVVGDWLGDARENDVFEHAGARDVIRREDFAKTGATTMREVLNRI
                     PGVSAPENNGTGSHDLAMNFGIRGLNPRLASRSTVLMDGIPVPFAPYGQPQLSLAPVS
                     LGNMNAIDVVRGGGAVRYGPQSVGGVVNFVTRAIPQDFGIEAGVEGQLSPTSSQNNPK
                     ETHNLMVGGTADNGFGTALLYSGTRGSDWREHSATRIDDLMLKSKYAPDEVHTFNSLL
                     QYYDGEADMPGGLSRADYDADRWQSTRPYDRFWGRRKLASLGYQFQPDSQHKFNIQGF
                     YTQTLRSGYLEQGKRITLSPRNYWVRGIEPRYSQIFMIGPSAHEVGVGYRYVNESTHE
                     MRYYTATSSGQLPSGSSPYDRDTRSGTEAHAWYLDDKIDIGNWTITPGMRFEHIESYQ
                     NNAITGTHEEVSYNAPLPALNVLYHLTDSWNLYANTEGSFGTVQYSQIGKAVQSGNVE
                     PEKARTWELGTRYDDGALTAEMGLFLINFNNQYDSNQTNDTVTARGKTRHTGLETQAR
                     YDLGTLTPTLDNVSIYASYAYVNAEIREKGDTYGNLVPFSPKHKGTLGVDYKPGNWMF
                     NLNSDFQSSQFADNANTVKESADGSTGRIPGFMLWGARVVYDFGPQMADLNLAFGVKN
                     IFDQDYFIRSYDDNNKGIYAGQPRTLYMQGALKF"
     gene            complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="KLK57_003410"
     CDS             complement(6580..7533)
                     /gene="fecR"
                     /locus_tag="KLK57_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fec operon regulator FecR"
                     /protein_id="HBE0893693.1"
                     /translation="MNPLLTDSRRQALRSASHWYAVLSGERVSPQQEARWQQWYEQDQ
                     DNQWAWQQVENLRNQLGGVPGDVASRALHDTRLTRRHVMKGLLLLLGAGGGWQLWQSE
                     TGEGLRADYRTAKGTVSRQQLEDGSLLTLNTQSAADMRFDAHQRTVRLWYGEIAITTA
                     KDALQRPFRVLTRQGQLTALGTEFTVRQQDNFTQLDVQQHAVEVLLASAPAQKRIVNA
                     GESLQFSASEFGAVKPLDDESTSWTKDILSFSDKPLGEVIATLTRYRNGVLRCDPAVA
                     GLRLSGTFPLKNTDAILNVIAQTLPVKIQSITRYWINISPL"
     gene            complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="KLK57_003411"
     CDS             complement(7530..8051)
                     /gene="fecI"
                     /locus_tag="KLK57_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FecI"
                     /protein_id="HBE0893694.1"
                     /translation="MSDRATTTASLTFESLYGTHHGWLKSWLTRKLQSAFDADDIAQD
                     TFLRVMVSETLSTIRDPRSFLCTIAKRVMVDLFRRNALEKAYLEMLALMPEGGAPSPE
                     ERESQLETLQLLDSMLDGLNGKTREAFLLSQLDGLTYSEIAHKLGVSISSVKKYVAKA
                     VEHCLLFRLEYGL"
     gene            8343..9040
                     /locus_tag="KLK57_003412"
                     /pseudo
     CDS             8343..9040
                     /locus_tag="KLK57_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT         1987 a         2508 c         2585 g         1992 t
ORIGIN      
        1 cactttttag aaaaatcgat ggtcagaaac tggattagca attccgttcc agggttgctt
       61 ttgatttacg ttggcgtctg atcattgatt tatcctcaaa agcccaacct cattggtaat
      121 gaaccagctc cgtgaatgtc cgctctggca cagagcgaaa ttttttgatc tcccccccct
      181 gaaatctaaa cttagtcatg tcacgttttt gggtttctaa aattttaact tcgcgttttt
      241 cgttgccgta agggttatac agaaatgtcc gttaagcaga gttcaaaatt gattgccgtg
      301 atcacgactg gtttgaaagc cgcgcccaag cctgtacagc tctggtttgc gttgattatg
      361 aacctgtcag cctaaagcaa gcggatggac gatgagtatt ggtaatcttt cagagtccgg
      421 aaaagttcag ccccagtctg aacaggcttg ctggcgccag tccagtttca ttcagtcgtg
      481 gtttggttct tacggcctgt gcaatctacc tcattaggca catcggcctg ccagataccg
      541 gctcggggtg tatttccgct tccacgctga atactgttct cagcaatcct ggggtcatca
      601 cctcttctgg tgtgccttgc gccataacat gtccgtttgc cattaccacc agttgatcgc
      661 agtaccggct agcctgatta aggtcgtgca gcacagcgac caccgttttc ccctgagtcc
      721 ggagttcgcc catcaaccgc atcaggtcca cctggtgatt gatatcaaga taggtggttg
      781 gctcatcaag taatacaacg ggcgtattct gggccaggac cattgccaga aatgcgcgct
      841 ggcgctgacc gccggaaagc tcggttaacc gacgaacggc aagatgattg atccgggtct
      901 ggttcatggc gacattaact cgtgcattgt cttcagcgga gagacgcccc cagagtgaca
      961 gccagggatt acgaccatac gaaaccagct cctggactgt gatcccctct ggcgttaaat
     1021 ggtgctgagg cagcagcgaa agcctgcggg ccaactggcg cgatgagagc atatttatgg
     1081 gattatcgcc gagaaatacg gtgccagact gcggcattaa aagccgcgaa aaacagttta
     1141 acagcgtcga tttcccgcaa ccgttaggac cgatcagggc ggtgatcttc cccgttggca
     1201 gtgagagtga aacgtcgtta agtaccttgt ctgttccgta attgaccgtc agattttcag
     1261 ttcgtaaagt catttatcgc attctcacaa gcaaccagac aaaccacggc gcaccgataa
     1321 tggcggtcag cacgccaacc gggagctcca gtgggggatg aataattctc gccagcagat
     1381 cggcaaccac caacagcaac gcacctgtca gggccgaaac aggcagcagt ctgcggtgac
     1441 gtccaccggt gatgctacgc atcatatgcg gcaccacgag accaataaag ctaatcgggc
     1501 cgcaggcggc cacgccggta gatgtcatgg cgacagctag taacaaagcc cagaatcggg
     1561 tatagggcac cgacacaccg agcgtggtgg cgcgcgcatc gccgagtgca aggaggtcga
     1621 gatcgcggca aaaactcagg ctcagcggca gaaataaaat catcagcggg atggcaatct
     1681 tcacaaagct ccagtcacgg ccccataagc tgccggtcag ccacagcagg gcgttgttca
     1741 catcctgcgg gcgcgagagc atcagataat ccgtcaggct ggcccagcat gcagaaagcg
     1801 ccacgccggt gagtgccagc ttcatcggct ggtgggtctt tgccagcatc ttcagcaata
     1861 tcaaccccgc catgccgccc gcaaaggcca gcagcggcag caccatcacg ggcagtgacg
     1921 gcataagaag tagagccccc acagaggcca ggctggcggc atggttaacg ccgagaatat
     1981 ccggtgatgc cagagggttg cgcacaatcc cctgtatcag cacgcccgcc acggcgaggg
     2041 ctgcaccgac aaacagtgcc agcagcaagc gcggcagtcg gtactccatc aatacataat
     2101 aatgctcgcg tccggcctgc cagtcggtca gcagcgcgcg ccacggcacg gggatcactc
     2161 ccatatggag tgataacagc gcacagcccg ccagggcaag ggtgatgaaa ataaccagcg
     2221 caattttcat cctcgcctcc tcacaagcca gacaaagcaa gggctgccaa tcagcgccag
     2281 cactgcgcct gcgggcagat ctccggggaa ggccagcgcg cgtgcgagta catctgccag
     2341 cagcatcagc gtggccccca gcagcatgct cactggcagt acgttgcgct gatcgaagcc
     2401 tgcccagaag cgcgccagat gtggcaccag cagaccgata aacgccaccg gacctgccac
     2461 gctgacgcac gcaccaacca gaagcagcac taacatattg atgaccaaac gtagcctcgt
     2521 caggttcact cccagcgtat gggcggtgct gtcgctgagg ttgagcaggt tcagttgatt
     2581 cgccagcagc aacacgacag ggactgcagt gaccaccacc ggcaagagct gccagacatc
     2641 ctgccagcgg gcgtgggaca ctccgccagc cagccagtaa aagatgccgt aagcatgatc
     2701 ttcggccagc agcagggtga tgcgggtaag gcccatacaa aaggccgaca gcgcgatacc
     2761 cgcgaggatc agtttgtttc tgtcatgggt atgacgaaat ccgcctcctg cggtcatgac
     2821 cagcagccag ctcacgccgc ccccgcatgc cgcgatgaac gacagagaat agcctgcaat
     2881 cggcgtcgga ctcagcgcgc tggtaagcgc catagccagc gccgcgccgc tgttaatgcc
     2941 gagcagtgaa ggagaggcca ttgggttgtg ggtcagggtt tgcagcagcg tgcccgcgag
     3001 cgccaggctt gcgccgatca gaacggcgac caggcttcgt ggcaaacgaa ggttttgcac
     3061 cagcgcttct ggtagcgttg gcgtgtgtcc aggcagcagg gcgcgggttg catctgctcc
     3121 ggaaacagga atggccgagt agcaaaacag actcagccag aaaataataa taagtgctgc
     3181 aacgggaagc ccccacagca gcaccgggtg ttttatcgcg gtcatttcac aacggtaagc
     3241 ggctgatggt ggaagatttt taccgtgtca gcggcaatac gctctgcagc aaaaataccg
     3301 cgcatccgcg cccaggtgtt actgtcgacc gaagcaacct gctgcttctg cgcggcggtt
     3361 aacatctgcc agagcggatc ttgttgccag cgtttaacaa tgctctcttc gcgatagtgg
     3421 gcaaccagca gccaggcagg attgaccgcc agcagttgct ccaggccgat ggacggcatg
     3481 gacgcacccg ccatcgcagc gggaacgttc agccccagag aggccagcac gctgccggtc
     3541 caggtctcct gagtatgcag gttgaattgc tgttcgcgtg atgtgccaaa ggccacgcgt
     3601 gtccctttgg gaagctggct ggcccactgc gccatcctct ctttatgttg ttccagacgt
     3661 gcctgcatct ctcgcttttt acccaccatt tcgccgatga tagccgcaga ttgcaaattt
     3721 tcagcgtagg tttcgttgcg ggacttaagc agcagtaccg acgcgatttg ctgcaaggcg
     3781 atgtaaaccc ccgcatggcg actgctgtcg gcaatgatca ggtctggttt cagagcggca
     3841 atggcttcca ggctcggctg cgcgcgcgtt ccgacggact gccacggttt caggtgcgca
     3901 cgcacttcgg gcaggatgcg ttttgcatcg ttatcgtcgg caataccgat cgggctgacg
     3961 tccacggcgg ccagcgcatc ggcgaacgag agttccagca ccacaatccg ttgtggcgtt
     4021 ttttcgagtg taaacgtgcc gtgttcgtcc tgaaccgtgg cggcaaaggc gtggctgatc
     4081 accagcagca ggcctgcaaa aagaaaacgg ataaatgcca acataatcac attccagcta
     4141 aaagcccggc aagccgggcg ttaacacatc agaacttcaa cgccccctgc atatacagcg
     4201 tgcgcggctg gcctgcatag atgcctttgt tgttgtcgtc ataagagcgg atgaagtagt
     4261 cctggtcgaa gatgtttttc acaccgaatg ccaggttcag atctgccatc tgcgggccaa
     4321 agtcatacac cacgcgtgcg ccccagagca tgaagccggg aatgcggccg gtactgccgt
     4381 cggcgctctc tttcaccgta ttggcgttat ccgcaaactg gctggactgg aaatcgctgt
     4441 tcagattgaa catccagttt cctggcttgt agtccacgcc cagcgtgcct ttatgtttcg
     4501 gggagaatgg taccagattg ccgtaggtgt cgcctttctc gcggatttcc gcgttcacat
     4561 acgcatagct ggcgtagatg gaaacgttat caagcgttgg cgttagcgta cccagatcgt
     4621 aacgtgcctg cgtttccagc ccggtatggc gcgttttgcc acgtgcagtg acggtgtcgt
     4681 tggtctggtt ggagtcgtac tgattgttaa agttaatcag gaacagcccc atttccgccg
     4741 tcagcgcgcc gtcgtcgtag cgggtaccga gttcccaggt tcgcgctttt tccggttcca
     4801 cattgccgct ttgcacagcc ttgccaatct ggctgtactg tacggtgccg aacgagcctt
     4861 cagtgtttgc ataaagattc cagctgtcag tcaggtgata gagcacgttc aacgccggaa
     4921 gcggtgcgtt atagctcact tcttcgtgcg tgcctgtgat ggcgttgttc tggtatgact
     4981 cgatatgttc gaaacgcata cccggcgtga tggtccagtt gccgatgtcg attttgtcat
     5041 ccagatacca ggcgtgcgcc tcggtgccgg aacgcgtatc gcggtcgtaa gggcttgagc
     5101 cggacggcaa ctgcccgctg ctggtggcgg tgtagtaacg catttcatgc gttgattcat
     5161 tcacatagcg atagcccacg cccacttcgt gcgcggaagg gccgatcata aagatctggc
     5221 tgtagcgtgg ctcaataccg cgcacccagt agttacgcgg cgagagggtg atgcgtttgc
     5281 cttgctccag gtagccgctg cgcagggttt gggtgtagaa cccctgaatg ttgaatttat
     5341 gctggctgtc tggctggaac tggtagccca ggctcgccag cttgcgacga ccccagaagc
     5401 ggtcatacgg gcgggtggat tgccagcgat cggcgtcgta atccgcgcga gacaggccac
     5461 cgggcatgtc ggcttcaccg tcgtaatatt gcagcaggct gttgaaggtg tgcacctcat
     5521 ccggcgcata tttgcttttc agcatcaggt cgtcgatgcg ggtggcgctg tgctcgcgcc
     5581 agtcactgcc gcgcgtgccg gagtagagca gcgcggtgcc aaaaccgttg tccgctgtgc
     5641 cgcccaccat caggttgtgc gtctctttcg ggttgttttg tgaagaggtt gggctgagct
     5701 gaccttccac gcccgcctcg ataccaaagt cctgcggaat ggcacgggta acaaagttca
     5761 ccacgccgcc cacgctctgc ggtccgtaac gcaccgcacc accaccgcgt accacgtcaa
     5821 tggcattcat gttgccgagc gaaacgggag ccagtgaaag ctgcggctga ccgtaagggg
     5881 cgaaggggac ggggatgccg tccatcagga cggtcgagcg gctggcgagg cgcgggttca
     5941 ggccccggat gccaaagttc atcgccaggt cgtggctgcc ggtgccgttg ttttccggcg
     6001 cgctgacgcc agggatgcgg ttaagtacct cacgcatggt ggttgcgccg gttttggcga
     6061 aatcctcacg gcggatcacg tcacgcgcgc cagcatgttc aaatacgtcg ttttcacgcg
     6121 catcacccag ccagtcgccg accacggtca gggcatcttc ttttggtgcg ggcgcgggct
     6181 ccagcgtcca gctgttattt cccagcggtt ttacctgcag tccgctgccg tccagcagtt
     6241 gttgcaggcc gctctcgacg tcgtaatcgc cgtgcaggcc gttgctctgc ttgccgcgcg
     6301 tcaggctggc gtcaaccgag agggtaaatc cgctgtgtgc ggcatactga ttgagcgctt
     6361 tatcgagcga tcccggtgcg atattaacct gtgcagcaaa agcggaaaac gagagaccgg
     6421 ccagcggcag caggctcagg cgaatggcgt taaccaaagg tgttgtttta cgaaaaacgc
     6481 gtaacggcgt cataccttcc ccatcatcat ttttgttgtg ttcagctatg agtcgaacga
     6541 gaagggtaaa aaggacaatc gaaataagaa ttattttcct tacagtggtg aaatgtttat
     6601 ccagtaccgc gtaatagact gaattttaac gggaagcgtt tgcgcgataa cgttcaggat
     6661 cgcatcggta tttttcagcg ggaacgtccc gctcaggcgc agcccggcaa cggcgggatc
     6721 gcagcgcagc acgccgttgc ggtaacgggt tagcgtggct atcacctcac ccagcggttt
     6781 atcgctgaag ctcaggatgt ccttcgtcca gcttgtactc tcgtcatcca gcggtttcac
     6841 tgcgccaaac tcagaggcgc tgaactgcag gctttcacca gcgttcacga tgcgtttttg
     6901 cgcgggggca ctggcgagaa gcacttccac ggcgtgctgc tgcacgtcaa gctgcgtgaa
     6961 attatcctgc tggcggacgg taaattctgt ccctaaagca gtgagctggc cctgacgggt
     7021 caggacacgg aaggggcgtt gcagggcatc tttcgcggta gtaatggcga tttcaccgta
     7081 ccagagccgg acggtgcgct gatgcgcatc aaaacgcata tccgccgcgc tttgggtatt
     7141 cagcgtgagc agggagccat cttccagttg ctgacggctg actgtgcctt tggcggtgcg
     7201 gtaatctgcc cgcagacctt cgccggtttc cgactgccag agctgccagc ctccgccagc
     7261 gccgagcaac agcagtaatc ctttcatcac gtgacggcgg gtgaggcggg tatcgtgcaa
     7321 cgcccggctg gcaacgtcgc caggcacacc gccaagctgg ttgcgcaggt tttcaacctg
     7381 ctgccaggcc cactggttat cctgatcctg ttcataccac tgttgccagc gcgcttcctg
     7441 ttgtgggctg acgcgctcgc cgcttagcac ggcataccag tgggaagctg aacgcagcgc
     7501 ctgacggcgg gaatcggtta acaaaggatt cataacccat actccagacg gaacagcagg
     7561 cagtgctcga cggctttcgc cacgtatttt ttcacggagc tgatggaaac accgagtttg
     7621 tgcgcaatct cgctgtatgt cagaccatcc agttgcgaaa gcagaaacgc ttcacgtgtt
     7681 ttgccgttta gcccgtccag catgctgtcg aggagttgta gggtctcgag ttggctttcg
     7741 cgttcctcag gtgaaggcgc tcccccctcc ggcataagcg ccagcatctc cagatacgct
     7801 ttttccagcg cgtttcggcg aaacaggtcc accatcacgc gtttggcgat agtgcagagg
     7861 aaggagcgag gatcgcggat cgtcgagagc gtttcgctga ccattacccg caaaaaagtg
     7921 tcctgggcaa tgtcatctgc atcaaaagca gactggagtt tgcgcgtcag ccagcttttc
     7981 aaccagccgt gatgtgtgcc ataaagcgac tcgaatgtta aggaagctgt ggtagtggcg
     8041 cggtcagaca tgcggagtgc atcaaaagtt aattatcacg tagccatatt aatatgagaa
     8101 tagttatcat tacaattgga aataaaattg tttccaatag acatttttaa catgttgttt
     8161 ttctaagtgt tataaggtag gtataaaatg ggatggagcc tctgcttctg gcatgtgtcg
     8221 gtcagaatga ctcatgatgt ggtctgctat tattgacatc ctcactgccc taaaggatag
     8281 ggatttcggt aatgctgtca acttactgat ttagtgtatg atggtgattt taaggtgctt
     8341 gcgtggcttc catttccatc agatgtcctt cctgctccgc tactgaaggc gtggtgcgta
     8401 acggcaaaag cactgccgga catcagcgct atctctgctc tcattgccgt aaaacatggc
     8461 aactacagtt cacttacacc gcctctcagc ccggtaagca ccagaaaatc attgatatgg
     8521 ccatgaatgg cgtcggatgt cgcgccagtg cacgcattat gggcgttggc ctcaacacgg
     8581 ttttacgtca cttaaaaaac tcaggccgca gtcggtaacc tcgcgcatac aaccgggcag
     8641 tgatgtgatt gtctgcgctg aaatggacga acagtggggc tacgtcggtg ctaaatcacg
     8701 tcagcgctgg ctgttttacg cgtatgacag gatacggagg acggttgtgg cgcacgtctt
     8761 cggtgaacgc actctggcca cactggagcg tcttctgagc ctgctgtcgg cctttgaggt
     8821 cgtggtatag atgacggatg gctggccgct gtatgaatca cgcctgaagg gaaagctgca
     8881 cgttatcagc aagcgttaca ctcagcgcat tgagcgacat aatctgaatc tgagacaaca
     8941 tctggcaagg ctgggacgga agtcactgtc gttctcaaaa tcggtggagc tgcatgacaa
     9001 ggtcatcggg cattatctga acataaaaca ctatcagtaa gttggagtca ttacctcctc
     9061 ccgaactcaa aa
//
LOCUS       DAEEKQ010000155         8810 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.157, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000155 DAEEKQ010000000
VERSION     DAEEKQ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.157"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(1..766)
                     /locus_tag="KLK57_003413"
                     /pseudo
     CDS             complement(1..766)
                     /locus_tag="KLK57_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            1045..1998
                     /gene="tal"
                     /locus_tag="KLK57_003414"
     CDS             1045..1998
                     /gene="tal"
                     /locus_tag="KLK57_003414"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HBE0893695.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            2113..2700
                     /gene="mog"
                     /locus_tag="KLK57_003415"
     CDS             2113..2700
                     /gene="mog"
                     /locus_tag="KLK57_003415"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HBE0893696.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(2735..3301)
                     /gene="satP"
                     /locus_tag="KLK57_003416"
     CDS             complement(2735..3301)
                     /gene="satP"
                     /locus_tag="KLK57_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HBE0893697.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="KLK57_003417"
     CDS             complement(3450..4163)
                     /gene="msyB"
                     /locus_tag="KLK57_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HBE0893698.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVIQIACLRRMVSATQV"
     gene            complement(4189..4593)
                     /locus_tag="KLK57_003418"
     CDS             complement(4189..4593)
                     /locus_tag="KLK57_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HBE0893699.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4970..6886
                     /gene="dnaK"
                     /locus_tag="KLK57_003419"
     CDS             4970..6886
                     /gene="dnaK"
                     /locus_tag="KLK57_003419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HBE0893700.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6975..8105
                     /gene="dnaJ"
                     /locus_tag="KLK57_003420"
     CDS             6975..8105
                     /gene="dnaJ"
                     /locus_tag="KLK57_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HBE0893701.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(8209..8361)
                     /gene="mokC"
                     /locus_tag="KLK57_003421"
     CDS             complement(8209..8361)
                     /gene="mokC"
                     /locus_tag="KLK57_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HBE0893702.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
BASE COUNT         2333 a         2186 c         2259 g         2032 t
ORIGIN      
        1 aaggtatatc ccgccgcgcc gcctgccgct tcctgccagc caaaagcact ctcaaaaata
       61 gaccaaatga cgtggggaag ttgcccgata tttattacgc aaatcaccat gctggtcagc
      121 acccagatta tcgccatcaa cgggacaaac ccctgcatga gccgggcgac gccatgaaga
      181 ccgcgagtga ttgccagcag agcaaagaca gcgagaataa tgcctgtcac cagcggggga
      241 aaatcaaaag aaaactcagg gctcgggcaa cggcgttcgc ctgaactccg ctgaaaatta
      301 tgccgtaggc gatgagcaaa aagacggcga acagaacgcc catccaacgc atccccagcc
      361 cgcgcgccat ataccatgcc ggtcctccac gaaactgtcc attgacgtca cgttctttat
      421 aaagttgtgc aagggaacat tcggcaaacg aggtcgccat gccgataaac gcggcaaccc
      481 acatccaaaa gacggctcca ggtccaccgg cggtaatagc cagcgcaacg ccggccaggt
      541 tgccgctacc cacgcgcgcc gcaagactgg tacacaatga ctgaaatgag gttaaaccgc
      601 ctggctgtgg atgaatgcta tttttaagac ttttgccaaa ctggcggatg tagcgaaact
      661 gcacaaatcc ggtgcgaaaa gtgaaccaac aacctgcgcc gaagagcagg taaatcatta
      721 ccgatcccca aaggacgctg ttaataaagg agaaaaaatc tggcatgcat atccctctta
      781 ttgccggtcg cgatgacttt cctgtgtaaa cgttaccaat tgtttaagaa gtatatacgc
      841 tacgaggtac ttgataactt ctgcgtagca tacatgaggt tttgtataaa aatggcgggc
      901 gatatcaacg cagtgtcaga aatccgaaac agtctcgcct ggcgataacc gtcttgtcgg
      961 cggttgcgct gacgttgcgt cgtgatatca tcagggcaga ccggttacat ccccctaaca
     1021 agctgtttaa agagaaatac tatcatgacg gacaaattga cctcccttcg tcagtacacc
     1081 accgtagtgg ccgacactgg ggacatcgcg gcaatgaagc tgtatcaacc gcaggatgcc
     1141 acaaccaacc cttctctcat tcttaacgca gcgcagattc cggaataccg taagttgatt
     1201 gatgatgctg tcgcctgggc gaaacagcag agcaacgatc gcgcgcagca gatcgtggac
     1261 gcgaccgaca aactggcagt aaatattggt ctggaaatcc tgaaactggt tccgggccgt
     1321 atctcaactg aagttgatgc gcgtctttcc tatgacaccg aagcgtcaat tgcgaaagca
     1381 aaacgcctga tcaaactcta caacgatgct ggtattagca acgatcgtat tctgatcaaa
     1441 ctggcttcta cttggcaggg tatccgtgct gcagaacagc tggaaaaaga aggcatcaac
     1501 tgtaacctga ccctgctgtt ctccttcgct caggctcgtg cttgtgcgga agcaggcgtg
     1561 ttcctgatct cgccatttgt tggccgtatt cttgactggt acaaagcgaa tactgataag
     1621 aaagagtacg ctccggcaga agatccgggc gtggtttctg tatctgaaat ctaccagtac
     1681 tacaaagagc acggttatga aaccgtggtt atgggcgcaa gcttccgtaa catcggcgaa
     1741 attctggaac tggcaggctg cgaccgtctt accatcgcac cggcactgct gaaagagctg
     1801 gctgagagcg aaggggctat cgaacgtaaa ctgtcttaca ccggcgaagt gaaagcgcgt
     1861 ccggcgcgta tcactgagtc cgagttcctg tggcagcaca accaggatcc aatggcagta
     1921 gataaactgg cggaaggtat ccgtaagttt gctattgacc aggaaaaact ggaaaaaatg
     1981 atcggcgatc tgctgtaatc attcttagcg tgaccgggaa gtcggtcacg ctacctcttc
     2041 tgaagcctgt ctgtcactcc cttcgcagtg tatcattctg tttaacgaga ctgtttaaac
     2101 ggaaaaatct tgatgaatac tttacgtatt ggcttagttt ccatctctga tcgcgcatcc
     2161 agcggcgttt atcaggataa aggcatccct gcgctggaag aatggctgac atcggcgcta
     2221 accacgccgt ttgaactgga aacccgctta atccccgatg agcaggcgat catcgagcaa
     2281 acgttgtgtg agctagtgga tgaaatgagt tgccatctgg tgctcaccac gggcggaact
     2341 ggcccggcgc gtcgtgacgt aacgcccgat gcgacgctgg cagtagcgga ccgcgagatg
     2401 cctggctttg gtgaacagat gcgccagata agcctgcatt ttgtaccaac tgcgatcctt
     2461 tcgcgtcagg ttggtgtgat tcgcaaacag gcgctgatcc ttaacttacc cggtcagccg
     2521 aagtctatta aagagacgct ggaaggtgtg aaggacgctg cgggtaacgt tgtggtacac
     2581 ggtatttttg ccagcgtacc gtactgcatt cagttgctgg aagggccata cgttgaaacg
     2641 gcaccagaag tggttgctgc attcagaccg aagagtgcaa gacgcgacgt tagcgaataa
     2701 aaaaatcccc ccgagcgggg ggatctcaaa acaattagtg ggattcacca atcggcagaa
     2761 cggtgcgacc aaactgctcg ttcagtactt cacccatcgc cagatagatt gcactggcac
     2821 cacagatcag accaatccag ccggcaaagt ggatgattgc ggcgttaccg gcaatgttac
     2881 cgatcgccag cagggcaaac agcacagtca ggctaaagaa aacgaattgc agaacgcgtg
     2941 cgcctttcag cgtgccgaag aacataaaca gcgtaaatac gccccacaga cccaggtaga
     3001 caccaaggaa ctgtgcattt ggcgcatcgg tcagacccag tttcggcatc agcagaatcg
     3061 caaccagcgt caaccagaaa gaaccgtaag aggtgaatgc ggttaaaccg aaagtgttgc
     3121 cttttttgta ctccagcaga ccggcaaaaa tttgcgcgat gccgccgtag aaaatgccca
     3181 tggcaagaat aataccgtcc agagcgaaat aacccacgtt gtgcaggtta agcagaatgg
     3241 tggtcatgcc gaagcccatc aggcccagcg gtgccggatt agccaactta gtgttgccca
     3301 taattcctca aaaatcatca tcgaatgaat ggtgaaataa tttccctgaa taacagtagt
     3361 gttttcaggg cgcggcataa taatcagcca gtggggcagt gtctacgatc ttttgagggg
     3421 aaaatgaaaa ttttccccgg tttacggtgt cagacctgag tggcgctaac catccggcgc
     3481 aggcaggcga tttgtattac ggctggaatt gtcacgcgat aggcactgcc gctgaccgct
     3541 ttaaccccat ttaatgctgc gcccacaggg cctcccagac ccgcgccgcg cagcaaacca
     3601 tgcccaagca cgctcattgc tgcgtgggtg cgtaaaatgc gggtcaattg gctggaaagc
     3661 aaatgcgaca cgccttttgc caataatttg tctttcatca gcagcggcag cagctcttcc
     3721 agctcattca ccctggcatc gaccgcgtgc agaaactcct gcttatgttc ctcgtccata
     3781 ttcttccagg tgttacgcag aaattgttcc agtaactgtt gctcaatctc aaacgtagac
     3841 atctctttgt cggctttcag cttcaatcgt tttgaaacat cgagcaaaat ggcccgatac
     3901 aatttaccgt gtccgcgcag tttgttggcg atactatcgc caccaaaatg ctgtaattct
     3961 ccagcaatca gctgccagtt gcggcgatgt tgctcgggat gcccttccat cgatttaaac
     4021 agttcgttgc gcatcagtac gctggagagg cgagttttgc ctttttcatt atgggtgagc
     4081 aatcgggcga aatttgccaa ctgttcctca ctacaatgct gaagaaaatc cagatctgaa
     4141 tcattcaggt aattaacatt cattttttgt ggtttctata ttctggcgtt agtcgtcgcc
     4201 gataattttc agcgtggcca tatccgatga gttcaccgta tgaccggaaa aggtgatttt
     4261 tgagacgcag cgtttattgt cgttatcact gttaatgttg atccagtcag tggtttgccc
     4321 ttcttttatt tctgaaggaa tattcagact ctgactggcg ctacgggcgg ctttgaaata
     4381 aaccgatgca ccgcttaact gtaaatcgcc atggtcggca gagagttgta tgcgtttcac
     4441 aatgcgacaa acaggaagtt tcagcgccag atcgttggtt tcgttacgcg gcattgcaat
     4501 gacgccgagg agtttatggt cgtttgcctg cgccgtgcag cacagcatca ggctaatcgc
     4561 caggctggcg gaaatcgtaa aaacggattt cataaggatt ctcttagtgg gaagaggtag
     4621 agggatgaat acccactagt ttactgctga taaagagaag attcaggtac gtaatctttt
     4681 ctttttatta caattttttg atgaatgcct tggctgcgat tcattcttta tatgaataaa
     4741 attgctgtca attttacgtc ttgtcctgcc atatcgcgaa atttctgcgc aaaagcacaa
     4801 aaaatttttg catctccccc ttgatgacgt gggttacgac cccatttagt agtcaaccgc
     4861 agtgagtgag tctgcaaaaa aatgaaattg ggcagttgaa accagacgtt tcgcccctat
     4921 tacagactca caaccacatg atgaccgaat atatagtgga gacgtttaga tgggtaaaat
     4981 aattggtatc gacctgggta ctaccaactc ttgtgtagcg attatggatg gcaccactcc
     5041 tcgcgtgctg gagaacgccg aaggcgatcg caccacgcct tctattattg cctataccca
     5101 ggatggtgaa actctggttg gtcagccggc taaacgtcag gcagtgacga acccgcaaaa
     5161 caccctgttt gcgattaaac gcctgattgg ccgccgcttc caggacgaag aagtacagcg
     5221 tgatgtttcc atcatgccgt tcaaaattat tgctgctgat aacggcgacg catgggtcga
     5281 agttaaaggc cagaaaatgg caccgccgca gatttctgct gaagtgctga aaaaaatgaa
     5341 gaaaaccgct gaagattact tgggtgaacc ggtaactgaa gctgttatca ccgtaccggc
     5401 atactttaac gatgctcagc gtcaggcaac caaagacgca ggccgtatcg ctggtctgga
     5461 agtaaaacgt atcatcaacg aaccgaccgc agctgcgctg gcttacggtc tggacaaagg
     5521 tactggcaac cgtactatcg cggtttatga cctgggtggt ggtactttcg atatttctat
     5581 tatcgaaatc gacgaagttg acggcgaaaa aaccttcgaa gttctggcta ccaacggtga
     5641 tacccacctg gggggtgaag acttcgacag ccgtctgatc aactacctgg ttgaagaatt
     5701 caagaaagat cagggcattg acctgcgcaa cgatccgctg gcaatgcagc gcctgaaaga
     5761 agcggcagaa aaagcgaaaa tcgaactgtc ttccgctcag cagaccgacg ttaacctgcc
     5821 gtacatcact gcagatgcga ccggtccgaa acacatgaac atcaaagtga ctcgtgcgaa
     5881 actggaaagc ctggttgaag atctggtaaa ccgttccatt gagccgctga aagttgcact
     5941 gcaggacgct ggcctgtccg tatctgatat cgacgacgtt atcctcgtgg gtggtcagac
     6001 tcgtatgcca atggttcaga agaaagttgc tgagttcttt ggtaaagagc cgcgtaaaga
     6061 cgttaacccg gacgaagctg tagcaatcgg tgctgctgtt cagggtggtg ttctgactgg
     6121 tgacgtaaaa gacgtactgc tgctggacgt taccccgctg tctctgggta tcgaaaccat
     6181 gggcggtgtg atgacgacgc tgatcgcgaa aaataccact atcccgacca agcacagcca
     6241 ggtgttctct accgctgaag acaaccagtc tgcggtaacc atccatgtgc tgcagggtga
     6301 acgtaaacgt gcagctgata acaaatctct gggtcagttc aacctggatg gtatcaaccc
     6361 ggcaccgcgc ggcatgccgc agatcgaagt taccttcgat atcgatgctg acggtatctt
     6421 gcacgtttcc gcgaaagata aaaacagcgg taaagagcag aagatcacca tcaaggcttc
     6481 ttctggtctg aacgaagatg aaatccagaa aatggtacgc gacgcagaag ctaacgccga
     6541 agctgaccgt aagtttgaag agctggtaca gactcgcaac cagggcgacc atctgctgca
     6601 cagcacccgt aagcaggttg aagaagcagg cgacaaactg ccggctgacg acaaaactgc
     6661 tatcgagtct gcgctgactg cactggaaac tgctctgaaa ggtgaagaca aagccgctat
     6721 cgaagcgaaa atgcaggaac tggcacaggt ttcccagaaa ctgatggaaa tcgcccagca
     6781 gcaacatgcc cagcagcaga ctgccggtgc tgatgcttct gcaaacaacg cgaaagatga
     6841 cgatgttgtc gacgctgaat ttgaagaagt caaagacaaa aaataatcgc cctataaatg
     6901 ggtaattata ctgacacggg cgaaggggaa tttcctctcc gcccgtgcat tcatctaggg
     6961 acaatttaaa aaagatggct aagcaagatt attacgagat tttaggcgtt tccaaaacag
     7021 cggaagagcg tgaaatcaaa aaggcctaca aacgcctggc catgaaatac cacccggacc
     7081 gtaaccaggg tgacaaagag gccgaggcga aatttaaaga gatcaaggaa gcttatgaag
     7141 ttctgaccga ctcgcaaaaa cgtgcggcat acgatcagta tggtcatgct gcgtttgagc
     7201 aaggtggcat gggcggcggc ggttttggcg gcggcgcaga cttcagcgat atttttggtg
     7261 acgttttcgg cgatattttt ggcggcggac gtggtcgtca acgtgcggcg cgcggtgctg
     7321 atttacgcta taacatggag ctcaccctcg aagaagctgt acgtggcgtg accaaagaga
     7381 tccgcattcc gactctggaa gagtgtgacg tttgccacgg tagcggtgca aaaccaggta
     7441 cacagccgca gacctgtccg acctgtcatg gttctggtca ggtgcagatg cgccagggat
     7501 ttttcgctgt acagcagacc tgtccacact gtcagggccg cggtacgctg atcaaagatc
     7561 cgtgcaacaa atgtcatggt catggtcgtg ttgagcgcag caaaacgctg tccgttaaaa
     7621 tcccggcagg ggtggacact ggagaccgca tccgtcttgc gggcgaaggt gaagcgggcg
     7681 agcatggcgc accggcaggc gatctgtacg ttcaggttca ggttaaacag cacccgattt
     7741 tcgagcgtga aggcaacaac ctctattgcg aagtcccgat caacttcgct atggcggcgc
     7801 tgggtggcga aatcgaagta ccgacccttg atggtcgcgt caaactgaaa gtgcctggcg
     7861 aaacccagac cggtaagctt ttccgtatgc gcggtaaagg cgtcaagtct gtccgcggtg
     7921 gcgcacaggg tgatttgctg tgccgcgttg tcgtcgaaac accggtaggc ctgaacgaga
     7981 agcagaaaca gttgctgcaa gagctgcaag aaagcttcgg tggcccaacc ggtgagcaca
     8041 acagcccgcg ctcaaagagc ttctttgatg gtgtgaagaa gttttttgac gacctgaccc
     8101 gctaacctcc ccaaaagcct gcccgtgggc aggcttgggt aaaattaggg tgcgttgaag
     8161 atatgcgagc acctgaaaag tggcggggat cactccccgc cgttgctctt actcggattc
     8221 gtaagccgtg aaaacagcaa cctccgtctg gccagttcgg atgtgaacct cacagaggtc
     8281 ttttctcgtt accagcgccg ccactacggc ggtgatacag atgacgatca gggcgacaat
     8341 catcgcctta tgctgcttca ttgctctctt ctccttgacc ttacggtcag taagaggcac
     8401 tctacatgtg ttcagcatat agggggcctc gggttgatag taaaatatca ctcggggctt
     8461 ttctctatct gccgttcagc taatgcctga gacagatagc ctcaagcacc cgccgctatt
     8521 atatcgctct ctttaaccca ttctgtttta tcgattctaa tcctgaagac gcctcgccta
     8581 tttttgtggc gtaatttttt aatgatttaa ttatttaact ttaatttatc tatttatcgc
     8641 aattattgac gacaagctgg attatttttg aaatattgtc ctaacaaata acgccgactg
     8701 acaagaaatt aattattact tttcctaatt aatccctcag aaatcctgac cttaagctat
     8761 gattatcagg gcttagggtc acccgttagc gctgatatga ctttccgtgt
//
LOCUS       DAEEKQ010000156         8692 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.158, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000156 DAEEKQ010000000
VERSION     DAEEKQ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8692
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.158"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..413
                     /locus_tag="KLK57_003422"
     CDS             <1..413
                     /locus_tag="KLK57_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="surface exclusion protein"
                     /protein_id="HBE0893703.1"
                     /translation="GNAETIFMLFFIVLWQGLFIWLFSQIRKKRNVSDEFKFSKGVWY
                     ITMPVSSLLSPLLSLMVFIIGTLYELRRVSGCVSVREWMQSQVNEQTNEDLHLDFDNM
                     DFHRTNPATGLPMSGIGVDIGGNSYGYSSKRYDE"
     gene            462..1193
                     /locus_tag="KLK57_003423"
     CDS             462..1193
                     /locus_tag="KLK57_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement resistance protein TraT"
                     /protein_id="HBE0893704.1"
                     /translation="MKMKKLMMVALVSSTLALSGCGAMSTAIKKRNLEVKTQMSETIW
                     LEPASERTVFLQIKNTSDKDMSGLQGKIADAVKAKGYQVVTSPDKAYYWIQANVLKAD
                     KMDLRESQGWLNRGYEGAAVGAALGAGITGYNSNSAGATLGVGLAAGLVGMAADAMVE
                     DVNYTMITDVQIAERTKATVTTDNVAALRQGTSGAKIQTSTETGNQHKYQTRVVSNAN
                     KVNLKFEEAKPVLEDQLAKSIANIL"
     gene            1396..2133
                     /locus_tag="KLK57_003424"
     CDS             1396..2133
                     /locus_tag="KLK57_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893705.1"
                     /translation="MTSDNIAEYVGENNQFTEKPVTEYPPVIQDNTVTTKDYLYSNNI
                     DTDLLVVIGGLIVILFLMMLIMYFINSLRHKELTEILTKISFPEKSGSNLLKPEAVSC
                     NLLSLCVLAPVFCTPRKLAEVSREIIVASAQEIAWTPGSSRIDGHQAENITWAISSQS
                     PADKYDLINKTSCVSPDSGRFYEPDTVLDHRERVRLFIQIQFPHGSDWSEVLRLTDIV
                     TEKVSSGRTAGGHHHRHTGYTWSVSVN"
     gene            2184..4418
                     /gene="traD"
                     /locus_tag="KLK57_003425"
     CDS             2184..4418
                     /gene="traD"
                     /locus_tag="KLK57_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HBE0893706.1"
                     /translation="MSFNAKDMTQGGQIASMRIRMFSQIANIMLYCLFIFFWILIGLV
                     LWVKISWQTFINGCIYWWCTSLEGMRDLIKSQPVYEIQYYGKTFRMNAAQVLHDKYMI
                     WCGEQLWSAFVLASVVALVICLITFFVVSWILGRQGKQQSENEVTGGRQLTDNPKDVA
                     RMLKKDGKDSDIRIGDLPIIRDSEIQNFCLHGTVGAGKSEVIRRLANYARQRGDMVVI
                     YDRSGEFVKSYYDPSIDKILNPLDARCAAWDLWKECLTQPDFDNTANTLIPMGTKEDP
                     FWQGSGRTIFAEAAYLMRNDPNRSYSKLVDTLLSIKIEKLRTFLRNSPAANLVEEKIE
                     KTAISIRAVLTNYVKAIRYLQGIEHNGDPFTIRDWMRGVREDQKNGWLFISSNADTHA
                     SLKPVISMWLSIAIRGLLAMGENRNRRVWFFCDELPTLHKLPDLVEILPEARKFGGCY
                     VFGIQSYAQLEDIYGEKAAATLFDVMNTRAFFRSPSHKIAEFAAGEIGEKEHLKASEQ
                     YSYGADPVRDGVSTGKDMERQTLVSYSDIQSLPDLTCYVTLPGPYPAVKLSLKYQARP
                     KVAPEFIPRDINPEMENRLSAVLAAREAEGRQMASLFEPEVASGEDVTQAEQPQQPQQ
                     PQQPQQPQQPQQPQQPQQPQQPQQPQQPVSSVINDKKSDAGVSVPAGGIEQELKMKPE
                     EEMEQQLPPGISESGEVVDMAAYEAWQQENHPDIQQHMQRREEVNINVHRERGEDVEP
                     GDDF"
     gene            4418..>8692
                     /gene="traI"
                     /locus_tag="KLK57_003426"
     CDS             4418..>8692
                     /gene="traI"
                     /locus_tag="KLK57_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBE0893707.1"
                     /translation="MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRL
                     IDAHNQAVDFAVRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHV
                     VVANVTQHNGEWKTLSSDKVGKTGFSENVLANRIAFGKIYQSELRQRVEALGYETEVV
                     GKHGMWEMPGVPVEAFSSRSQAIREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAE
                     WMQTLKETGFDIRAYRDAADQRAEIRTQAPGPASQDGPDVQQAVTQAIAGLSERKVQF
                     TYTDVLARTVGILPPENGVIERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSV
                     RALSRDIMKQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVA
                     ELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQEGMVFTPGSTVIVD
                     QGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
                     PATIISEPDRNVRYDRLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVL
                     GHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLT
                     LRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASV
                     SEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMD
                     NATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQ
                     KTGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKR
                     GDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSG
                     QRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAV
                     GEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDMARAYALIA
                     AGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINR
                     DVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGETFPDV
                     PMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVP
                     VLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISKDDGLITLEDAEGNTRLIS
                     PREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQ
                     QTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKL"
BASE COUNT         2088 a         2107 c         2608 g         1889 t
ORIGIN      
        1 gggggaatgc cgaaacaatt tttatgctgt tcttcatagt actatggcag ggactgttta
       61 tatggttgtt ctctcaaata cgaaaaaaac ggaatgtgtc ggatgagttt aagtttagta
      121 aaggcgtttg gtatataacg atgccagtat cttccttact ctctccatta ttaagcctga
      181 tggtttttat cattggtaca ctatacgaat taaggcgagt tagtggttgt gtaagcgtaa
      241 gagaatggat gcaatctcag gtaaatgaac aaactaatga agatttgcat ctggattttg
      301 ataatatgga ttttcatcgt actaatcctg caacagggtt acctatgtca ggtattggtg
      361 tggatatcgg tggtaattca tatggttata gttcaaaacg atatgatgag tgaatcttaa
      421 tttgtatatt atgacgtttt attcaatatg aaggaacatt gatgaaaatg aaaaaattga
      481 tgatggttgc actggtcagt tccactctgg ccctttcagg gtgtggtgcg atgagcacag
      541 caatcaagaa gcgtaacctt gaggtgaaga ctcagatgag tgagaccatc tggcttgaac
      601 ccgccagcga acgcacggta tttctgcaga tcaaaaacac gtctgataaa gacatgagtg
      661 ggctgcaggg caaaattgct gatgctgtga aagcaaaagg atatcaggtg gtgacttctc
      721 cggataaagc ctactactgg attcaggcga atgtgctgaa ggccgataag atggatctgc
      781 gggagtctca gggatggctg aaccgtggtt atgaaggcgc agcagttggt gcagcgttag
      841 gtgccggtat taccggctat aactcaaatt ctgccggtgc cacactcggt gtaggccttg
      901 ctgctggtct ggtgggtatg gctgcagatg cgatggtgga agatgtgaac tataccatga
      961 tcacggatgt acagattgca gagcgtacta aggcaacggt gacaacggat aatgttgccg
     1021 ccctgcgtca gggcacatca ggtgcgaaaa ttcagaccag tactgaaaca ggtaaccagc
     1081 ataaatacca gacccgtgtg gtttcaaatg cgaacaaggt taacctgaaa tttgaagagg
     1141 cgaagcctgt tctcgaagac caactggcca aatcaatcgc aaatattctc tgaacctcgt
     1201 caggaggctg atctgaccgg cctccttgct gacgcctcgc gttgctcgtt gtccaacccc
     1261 gggatgtaac aaaataaatt ttgtcacctc ccggcgtttc cgaaataaat atgaaaacac
     1321 tgagcggttg tgcaaattct ttgtgcgacc gtgaagtgtt gccggcgaag ccgggatctt
     1381 ccggagaaag tcattatgac atcagacaat atcgctgaat atgttggtga gaataatcaa
     1441 tttactgaga agcctgtaac tgaatacccc ccggtgatac aggataatac agtgaccact
     1501 aaagactatc tgtacagcaa taatattgat actgaccttt tagtggttat cggagggctt
     1561 attgttatat tgtttctcat gatgcttatc atgtacttta ttaattctct caggcataag
     1621 gaattaactg aaatactgac aaaaatatct tttcctgaaa aatccgggag caatttgctt
     1681 aaaccggagg cggtcagctg taatttgttg tccctgtgtg tgctggcacc ggtattctgt
     1741 acacccagaa agctggctga agtttccagg gagatcatcg tggcgagtgc gcaggaaatt
     1801 gcatggacgc cggggagcag tcgtattgat ggccaccagg cggagaatat cacctgggcg
     1861 atttcatcgc agtctcctgc cgataagtat gatctcatca ataaaacgtc ctgtgtttct
     1921 cctgacagtg gccgttttta tgaaccggac acagttctgg atcaccgtga acgggtacgc
     1981 ctttttattc agatccagtt tccgcatggg agtgactggt ctgaagtatt gcgcctgact
     2041 gacattgtta cagagaaggt ctcttccggc aggactgcag gcgggcatca ccaccggcat
     2101 acagggtaca cctggtctgt ttctgtgaat taaccccaca tcatcctttt ctgccctgtt
     2161 ccagattttc ccggagtttc atcatgagtt ttaacgcaaa ggatatgacc cagggcggtc
     2221 agattgcgtc catgcgtatc cgcatgttca gccagatcgc caatatcatg ctttactgcc
     2281 tgtttatttt tttctggatc ctcattggtc tggtcttatg ggtaaaaata agctggcaga
     2341 cgtttatcaa cggctgtatt tactggtggt gtacgtcact ggaaggcatg cgggatttaa
     2401 taaaatccca gccggtctat gagatccagt attacggcaa aaccttccgg atgaacgctg
     2461 cccaggtact gcatgataaa tatatgatct ggtgcggaga gcagctatgg tctgcattcg
     2521 ttctggcgtc tgtcgtggcc ctggttattt gcctgatcac cttctttgtt gtctcctgga
     2581 ttctggggcg tcagggtaaa caacagagcg aaaatgaagt cacaggtggt cgtcagctga
     2641 cagacaatcc gaaagacgtt gcccggatgc tgaaaaaaga cggcaaggac tccgatatcc
     2701 ggattggcga cctgccgatt atccgggatt ccgaaatcca gaacttctgt ctgcacggca
     2761 cggtcggggc cggtaagtcg gaggttatcc gtcgtctggc caactatgcc cgtcagcgcg
     2821 gtgatatggt ggtgatttat gaccgttcag gggaatttgt taaaagttac tatgatccct
     2881 ccatcgataa aatcctgaat ccgctggatg cccgctgtgc cgcatgggat ttgtggaagg
     2941 agtgcctgac acagccggat tttgataata ccgcgaacac gctgatccct atgggtacaa
     3001 aagaggaccc gttctggcag ggttcaggac gtaccatttt tgcggaagcg gcgtacctga
     3061 tgcgtaatga ccccaaccgc agctacagca aactggtgga cacactgctt tccatcaaaa
     3121 ttgaaaaact gcgtaccttc ctgcgtaatt caccggcggc caacctggtg gaagagaaaa
     3181 ttgagaaaac ggcgatttcc atccgtgctg tgctgaccaa ctacgtgaaa gccatccgtt
     3241 acctgcaggg gattgagcat aacggtgatc ccttcaccat ccgtgactgg atgcgtggtg
     3301 tccgggaaga tcagaaaaac ggctggctgt ttatttcgtc gaacgccgac acccatgcct
     3361 ccctgaagcc tgtgatctcc atgtggctgt ccattgccat tcgtggtctg ctggcaatgg
     3421 gagaaaaccg taaccgtcgt gtgtggtttt tctgtgatga gttacccacg ttacacaaac
     3481 tgccggatct ggtggagatc ctgccggaag cccgtaagtt cggtggctgt tatgtgtttg
     3541 gtatccagtc ctatgcccag ctggaagata tctacggtga gaaagcggcg gccacgctgt
     3601 ttgacgtcat gaacacccgt gcttttttcc gttccccgag ccataagatt gcagagttcg
     3661 ctgccggtga gattggtgag aaagagcacc tgaaagccag cgagcagtat tcctacggtg
     3721 ctgacccggt acgtgacggg gtatcgaccg gtaaggatat ggagcgtcag acgctggtca
     3781 gttattccga cattcagtct ctgccggatc tgacctgcta tgtcactctg cccggaccgt
     3841 atccggcagt caaactctct ctgaaatatc aggcacgacc gaaggtcgct ccggagttta
     3901 ttccgcgtga catcaacccg gaaatggaga accgtctgag tgccgtactt gccgcaaggg
     3961 aagcagaagg tcgtcagatg gccagcctct ttgaaccgga ggttgcctcc ggagaagatg
     4021 tgactcaggc tgaacagcca caacagccac aacagccaca acagccacaa cagccacaac
     4081 agccacaaca gccacaacag ccacaacagc cacaacagcc acaacagcca caacagccag
     4141 tgtcttctgt catcaatgat aagaagtcag atgcaggtgt gagtgttccg gcagggggga
     4201 tcgagcagga gctgaaaatg aaaccggaag aagagatgga acagcaactg ccacccggga
     4261 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcaa caggaaaatc
     4321 atccggacat ccagcagcat atgcagcgtc gtgaagaggt gaacattaat gtgcaccggg
     4381 agcgcgggga ggatgttgag ccgggagatg atttctgatg ttgagtttca gcgttgttaa
     4441 atcggcaggc agtgccggaa actattacac cgacaaggat aattactatg tgctgggcag
     4501 catgggagaa cgctgggctg gtcagggggc tgaacagctt gggctgcagg gcagcgtcga
     4561 taaggatgtt tttacccgtc ttctggaggg caggctgccg gacggggcgg atttaagccg
     4621 catgcaggat ggcagtaata agcatcgtcc cggctacgac ctgaccttct ccgcccccaa
     4681 aagtgtctcc atgatggcca tgctcggggg ggataaacgt cttattgatg cacataacca
     4741 ggccgtggat tttgctgtcc gtcaggtgga ggcgctggct tccacacggg tgatgacgga
     4801 cggacagtca gaaacggtgc tgaccggtaa tctggtgatg gcactgttta accatgacac
     4861 cagtcgcgat caggacccac agttacacac gcatgtagtg gtggctaatg tcacgcagca
     4921 taacggcgag tggaagacac tgagcagtga caaagtgggg aaaaccggat tttccgagaa
     4981 cgtgctagcg aaccggattg cgttcgggaa aatatatcag agtgagttac gtcagcgcgt
     5041 tgaggcgctg ggttatgaaa ccgaagtggt cggtaagcac ggtatgtggg agatgccggg
     5101 cgtaccggtg gaggcctttt ccagtcgttc acaggctatc agggaggccg tcggtgaaga
     5161 cgcctcgctg aaatcccggg atgtggcggc actggatacg cgaaaatcca aacagcacgt
     5221 cgatcctgaa gtcagaatgg ctgaatggat gcagacgctg aaggaaaccg ggttcgacat
     5281 ccgggcatat cgtgacgcgg cggatcagcg tgcggagatc cgcacgcagg cgcccgggcc
     5341 tgcttcacag gacgggccgg atgtgcagca ggcggtgaca caggcgattg ccggattaag
     5401 tgaacgaaaa gtgcagttca cgtatacgga cgtgctggcc aggacggtcg gcatactgcc
     5461 gccggaaaat ggtgtgattg aacgggcacg cgccggtatc gatgaggcca tcagccgtga
     5521 gcagcttatc cccctcgacc gtgagaaggg gctgttcaca tcaggaattc atgtgctcga
     5581 tgagctgtcc gtccgggcac tcagtcgtga catcatgaaa cagaaccggg tgaccgtaca
     5641 tccggagaaa agtgtccccc ggacggctgg ttacagcgat gcggtcagtg ttctggcaca
     5701 ggaccgtccg tcgctggcca ttgtgtccgg gcagggcggt gcagccgggc agcgtgagcg
     5761 ggtggctgaa ctggtcatga tggcccggga gcaggggcgg gaggtgcaga ttatcgctgc
     5821 tgaccgtcgc tcgcagatga acctgaagca ggatgaacgg ctgtccggtg agctgataac
     5881 cggacgtcgt cagctgcagg agggcatggt cttcacgccg ggcagtactg ttatcgtcga
     5941 ccagggcgaa aaactctccc tgaaagagac gttaaccctg ctggatggtg ccgcacgtca
     6001 taacgtacag gtcctgataa ccgacagcgg gcagcgaacc ggtacaggca gtgcgctgat
     6061 ggccatgaag gatgccgggg tgaacacgta tcgctggcag ggcggagaac agcgaccggc
     6121 aaccatcatc agtgaaccgg accggaatgt ccgctatgac cggctggccg gagattttgc
     6181 ggccagcgtg aaagccggag aagagagcgt ggcacaggtc agcggggtac gggaacaggc
     6241 catactgaca caggccattc gcagtgagct gaaaacacag ggcgtgctcg gacacccgga
     6301 ggtgaccatg accgccctgt caccggtctg gctggacagc cggagccgtt atctgcggga
     6361 tatgtaccga ccggggatgg tgatggagca gtggaacccg gagacacgca gtcatgaccg
     6421 ctatgttatc gaccgggtga cggcgcagag tcacagcctg accctgcggg atgcgcaggg
     6481 cgaaacgcag gtggtgcgta tttcctccct ggacagcagc tggtcgctgt tccggccgga
     6541 aaaaatgccg gtggcagacg gcgagcgact gagggtgaca gggaaaattc ccggactccg
     6601 cgtctccggc ggtgaccgcc tgcaggtggc atccgtcagt gaagatgcga tgacggttgt
     6661 tgtgccgggg cgggctgaac cggccaccct gcctgtggct gattcaccgt tcacggcact
     6721 gaagctggag aacggctggg tggaaacgcc cgggcattcc gtcagtgaca gtgcgacggt
     6781 gtttgcctcc gtcacacaga tggcaatgga caacgccacc ctgaacggtc tggcccgcag
     6841 cggtcgtgat gtccggctgt attcctcact ggatgaaacc cgtactgcgg aaaaacttgc
     6901 ccgccatccc tcctttacgg tggtttctga gcagataaag gcgcgggccg gtgagacatc
     6961 gctggaaacc gctatcagtc tgcagaaaac cgggctgcac acgccggcac agcaggccat
     7021 tcatctggcc cttcctgtgc tggaaagtaa aaacctggcc ttcagcatgg tggacctgct
     7081 gacagaggcg aagtcgtttg ctgcagaagg aaccggtttt actgaactgg gaggggaaat
     7141 caatgcgcag ataaaacgcg gtgacttact gtatgtggat gtggcaaaag gctatggcac
     7201 aggcctgctg gtttcccgtg cgtcgtatga ggcagaaaaa agtattcttc gccatattct
     7261 cgaaggtaag gaggcggtca cgccgctgat ggagagagta cccggcgaac tcatggagac
     7321 gttaacgtcg ggacagcgtg ccgccacccg tatgatactg gaaacgtccg accgtttcac
     7381 ggtggtacag ggttatgccg gtgtgggtaa gaccacacag ttccgggcgg tgatgtcagc
     7441 cgtgaacatg ctgccggcga gtgagcgtcc ccgagttgtg gggctggggc ccacgcaccg
     7501 tgcggtcggt gagatgcgca gcgccggcgt ggatgcacag acgctggcgt cctttctgca
     7561 tgacacgcag ctgcagcagc gcagcggaga aacgccggat ttcagcaaca cgctgttcct
     7621 gctcgatgag agctcaatgg tgggcaatac cgacatggca cgggcatacg ccctgattgc
     7681 ggccggtggc ggtcgtgccg tggccagtgg tgacacggac cagctgcagg ccatcgcgcc
     7741 cggtcagcct ttccgtctcc agcagacgcg cagtgctgcc gatgtggtca tcatgaagga
     7801 gattgtgcgt cagacgccgg aactgcggga ggcggtatac agcctgatta accgggatgt
     7861 ggaaagggca ctgtccgggc ttgagagtgt gaaaccgtct caggttcccc gtcaggaggg
     7921 cgcatgggca ccggagcact ccgtgacgga gttcagtcac agccaggaag cgaaactggc
     7981 agaagcgcag cagaaggcga tgctgaaagg tgagactttt ccggatgtcc ccatgacact
     8041 gtatgaagcc attgtccgcg actataccgg caggacaccg gaagcacggg agcagacgct
     8101 gattgtcacg cacctgaatg aggaccggcg cgtactgaac agcatgattc atgatgcacg
     8161 ggaaaaggcc ggtgagctgg ggaaagagca ggtcatggtg cctgtcctga acacagcgaa
     8221 tatacgtgac ggggagctgc gtcgtctctc cacctgggag aataacccgg atgcccttgc
     8281 cctggtggat agtgtgtatc accggattgc cggtatcagc aaggatgacg ggctgataac
     8341 cctggaggat gcggaaggta acacgcggct gatttcgccc cgggaggcgg tggctgaagg
     8401 tgtcacactg tacaccccgg acaaaatccg ggtggggacc ggcgaccgga tgcgcttcac
     8461 gaagagtgac cgggagcgcg gttatgtggc caacagcgtc tggacggtga cagcggtttc
     8521 cggtgacagt gtcacgttgt cagacggaca gcagacccgg gtgattcgcc ccggccagga
     8581 gcgggcagag caacatattg acctggctta tgccatcacc gcccacggtg cgcagggggc
     8641 gagtgaaacc tttgccatcg cgcttgaagg cacggaaggt aaccggaaac tg
//
LOCUS       DAEEKQ010000157         8637 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.159, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000157 DAEEKQ010000000
VERSION     DAEEKQ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8637)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8637)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8637
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.159"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(246..509)
                     /gene="rpsT"
                     /locus_tag="KLK57_003427"
     CDS             complement(246..509)
                     /gene="rpsT"
                     /locus_tag="KLK57_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HBE0893708.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            612..830
                     /locus_tag="KLK57_003428"
     CDS             612..830
                     /locus_tag="KLK57_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HBE0893709.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            838..1779
                     /gene="ribF"
                     /locus_tag="KLK57_003429"
     CDS             838..1779
                     /gene="ribF"
                     /locus_tag="KLK57_003429"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HBE0893710.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKASMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1822..4638
                     /gene="ileS"
                     /locus_tag="KLK57_003430"
     CDS             1822..4638
                     /gene="ileS"
                     /locus_tag="KLK57_003430"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HBE0893711.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4638..5132
                     /gene="lspA"
                     /locus_tag="KLK57_003431"
     CDS             4638..5132
                     /gene="lspA"
                     /locus_tag="KLK57_003431"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HBE0893712.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSR
                     AKKQ"
     gene            5257..5706
                     /gene="fkpB"
                     /locus_tag="KLK57_003432"
     CDS             5257..5706
                     /gene="fkpB"
                     /locus_tag="KLK57_003432"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HBE0893713.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5708..6658
                     /gene="ispH"
                     /locus_tag="KLK57_003433"
                     /gene_synonym="lytB"
     CDS             5708..6658
                     /gene="ispH"
                     /locus_tag="KLK57_003433"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HBE0893714.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKRAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6724..7638
                     /gene="rihC"
                     /locus_tag="KLK57_003434"
     CDS             6724..7638
                     /gene="rihC"
                     /locus_tag="KLK57_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HBE0893715.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPIGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSSGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7805..8626
                     /gene="dapB"
                     /locus_tag="KLK57_003435"
     CDS             7805..8626
                     /gene="dapB"
                     /locus_tag="KLK57_003435"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HBE0893716.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKENGLFDMRDVLNLNNL"
BASE COUNT         1968 a         2159 c         2457 g         2053 t
ORIGIN      
        1 atccacattc tttgtagggg agtctggaga ttgagtagat attcttgttc agaatgtatc
       61 agccgatggt tctacgattc ttaagccacg aagagttcag aaagtgtaac ggcatgtctg
      121 caaaagccag catcttttga ctatctggca accggtagtg tgttctctca cgcatcataa
      181 aagcagcagg cataaaaaaa acccgcttgc gcgggctttt tcacaaagct tcagcaaatt
      241 ggcgattaag ccagtttgtt gatctgtgca gtcaggttag ccttatgacg tgcagctttg
      301 tttttgtgga tcagaccttt agcagcctga cggtccacga tcggttgcat ttcgttaaat
      361 gctttctgtg cagcagcttt gtcgccagct tcgatagctg cgtatacttt cttgatgaaa
      421 gtacgcatca tagagcgacg gcttgcgttg tgcttacgag ccttttcaga ctgaatggcg
      481 cgcttcttag ctgatttgat attagccaag gtccaactcc caaatgtgtt ctatatggac
      541 aattcaaagg ccgaggaata tgccctttta gccttctttt gtcaatggat ttgtgcaaat
      601 aagcgccgtt aatgtgccgg cactcgttac gtagtgatgg cgcaggattc taccagcttg
      661 cggggtgtga atacagcttt tccgcgataa aaattgcagc aggcggtcac tttcttcccg
      721 tgatttgcgc catggcaatg aaaagccact tctttctgat ttcggtactc aatcgccggt
      781 taaccttgac cgctgtacaa ggtatactcg gacgattttc actgttttga gccagacatg
      841 aagctgatac gcggcataca taatctcagc caggccccgc aagaagggtg tgtgctgact
      901 attggtaatt tcgacggcgt gcatcgcggt catcgcgcgc tgttacaggg cttgcaggaa
      961 gaagggcgca agcgcaactt accggtgatg gtgatgcttt ttgaacctca accactggaa
     1021 ctgtttgcta ccgataaagc cccggcaaga ctgacccggc tgcgggaaaa actgcgttac
     1081 cttgcagagt gtggcgttga ttacgtgctg tgcgtgcgtt tcgacaggcg tttcgcggcg
     1141 ttaaccgcgc aaaatttcat cagcgatctt ctggtgaagc atttgcgcgt aaaatttctt
     1201 gccgtagggg atgatttccg ctttggcgct ggtcgtgaag gcgatttctt gttattacag
     1261 aaagctagca tggaatacgg cttcgatatc actagtacgc aaactttttg cgaaggtggc
     1321 gtgcgtatta gcagcaccgc tgtgcgtcag gcgctggcgg atgacaatct ggctctggca
     1381 gaaagtttac tggggcaccc gtttgccatc tccgggcgtg tagtccacgg tgatgaatta
     1441 gggcgcacta taggtttccc gacggcgaat gtaccgctac gccgtcaggt ttccccggtg
     1501 aaaggggttt atgcggtaga agtgctgggc ctcggtgaaa agccgttacc cggcgtggca
     1561 aacatcggaa cacgcccaac ggttgccggt attcgccagc agctggaagt gcatttgtta
     1621 gatgttgcaa tggaccttta cggtcgccat atacaagtag tgctgcgtaa aaaaatacgc
     1681 aatgagcagc gatttgcgtc gctggacgaa ctgaaagcgc agattgcgcg tgatgaatta
     1741 accgcccgcg aattttttgg gctaacaaaa ccggcttaag cctgttatgt aatcaaaccg
     1801 aaacacggaa ccgagaatct gatgagtgac tataaatcaa ccctgaattt gccggaaaca
     1861 gggttcccga tgcgtggcga tctcgccaag cgcgaaccgg gaatgctggc gcgttggact
     1921 gatgatgatc tgtacggcat catccgtgcg gctaaaaaag gcaaaaaaac cttcattctg
     1981 catgatggcc ctccttatgc gaatggcagc attcatattg gtcactcggt taacaagatt
     2041 ctgaaagaca ttatcgtgaa gtccaaaggg ctttccggtt atgactcgcc gtatgtgcct
     2101 ggctgggact gccacggtct gccgatcgag ctgaaagtag agcaagaata cggtaagccg
     2161 ggtgagaaat tcaccgccgc cgagttccgc gccaagtgcc gcgaatacgc ggcgacccag
     2221 gttgacggtc aacgcaaaga ctttatccgt ctgggcgtgc tgggcgactg gtcgcacccg
     2281 tacctgacca tggacttcaa aactgaagcc aacatcatcc gcgcgctggg caaaatcatc
     2341 ggcaacggtc acctgcacaa aggcgcgaag ccagttcact ggtgcgttga ctgccgttct
     2401 gcgctggcgg aagcggaagt tgagtattac gacaaaactt ctccgtccat cgacgttgct
     2461 tttcaggcgg tcgatcagga tgcactgaaa gcaaaatttg ccgtaagcaa cgttaacggc
     2521 ccaatctcgc tggtgatctg gaccactacg ccgtggactc tgcctgcgaa ccgcgcaatc
     2581 tctattgcac ctgatttcga ctatgcgctg gtgcagatcg acggtcaggc cgtgattctg
     2641 gcgaaagatc tggttgaaag cgtaatgcag cgtatcggcg tgaccgatta caccattctc
     2701 ggcacggtaa aaggtgcgga gcttgagttg ctgcgcttta cccatccgtt tatgggcttc
     2761 gacgttccgg caatcctcgg cgatcacgtt accctagatg ccggtaccgg tgccgttcac
     2821 accgcgcctg gccacggccc ggacgactat gtgatcggtc agaaatacgg cctggaaacc
     2881 gctaacccgg ttggcccgga cggcacttat ctgccgggca cttatccgac gctggatggc
     2941 gtgaacgtct tcaaagcgaa cgacatcgtc gttgcgctgc tgcaggaaaa aggcgcgctg
     3001 ctgcacgttg agaaaatgca gcacagctat ccgtgctgct ggcgtcacaa aacgccgatc
     3061 atcttccgcg cgacgccgca gtggttcgtc agcatggatc agaaaggtct gcgtgcgcag
     3121 tcactgaaag agatcaaagg cgtgcagtgg atcccggact ggggccaggc gcgtatcgag
     3181 tcgatggttg ctaaccgtcc tgactggtgt atctcccgtc agcgcacctg gggcgtaccg
     3241 atgtcactgt tcgtgcacaa agacacggaa gagctgcatc cgcgtactct cgaactaatg
     3301 gaagaagtgg caaaacgcgt tgaagttgac ggcatccagg cgtggtggga tcttgatgcg
     3361 aaagagatcc tcggcgatga agctgatcag tacgtgaaag tgccggacac attggatgta
     3421 tggtttgact ccggatctac ccactcttct gttgttgacg tgcgtccgga atttgccggt
     3481 cacgcagcgg acatgtatct ggaaggttct gaccagcacc gtggttggtt catgtcttcc
     3541 ctaatgatct ccaccgcgat gaaaggcaaa gcgccgtatc gtcaggtact gacccacggc
     3601 tttaccgtgg atggtcaggg tcgcaagatg tctaaatcca tcggcaatac cgtttcgccg
     3661 caggatgtga tgaacaaact gggcgcggat attctgcgtc tgtgggtggc atcaaccgac
     3721 tacaccggtg aaatggccgt ttctgacgag atcctgaaac gtgctgccga tagctatcgt
     3781 cgtatccgta acaccgcgcg cttcctgctg gcaaacctga acggttttga tccagcaaaa
     3841 gatatggtga aaccggaaga gatggtggta ctggatcgct gggccgtagg ttgtgcgaaa
     3901 gcggcacagg aagacatcct caaggcgtac gaagcatacg atttccacga agtggtacag
     3961 cgtctgatgc gcttctgctc cgttgagatg ggttccttct acctcgacat catcaaagac
     4021 cgtcagtaca ccgccaaagc ggacagtgtg gcgcgtcgta gctgccagac tgcgctgtat
     4081 cacatcgcag aagcgctggt gcgctggatg gcaccaatcc tctccttcac cgctgatgaa
     4141 gtgtggggct acctgccggg cgaacgtgaa aaatacgtct tcaccggtga gtggtacgaa
     4201 ggcctgtttg gcctggcaga cagtgaagcg atgaacgatg cgttctggga cgagctgttg
     4261 aaagtgcgtg gcgaagtgaa caaagtcatt gagcaagcgc gtgccgacaa gaaagtgggt
     4321 ggctcgctgg aagcggcagt aaccttgtat gcagaaccgg aactgtcggc gaaactgacc
     4381 gcgctgggcg atgaattacg atttgtcctg ttgacctccg gcgctaccgt tgcagactat
     4441 aacgacgcac ctgctgatgc tcagcagagc gaagtactca aagggctgaa agtcgcgttg
     4501 agtaaagccg aaggtgagaa gtgcccacgc tgctggcact acacccagga tgtcggcaag
     4561 gtggcggaac acgcagaaat ctgcggccgc tgtgtcagca acgtcgccgg tgacggtgaa
     4621 aaacgtaagt ttgcctgatg agtcaatcga tctgttcaac agggctacgc tggctgtggc
     4681 tggtggtagt cgtgctgatt atcgatctgg gcagcaaata cctgatcctc cagaactttg
     4741 ctctggggga tacggtcccg ctgttcccgt cgcttaatct gcattatgcg cgtaactatg
     4801 gcgcggcgtt tagtttcctt gccgatagcg gcggctggca gcgttggttc tttgccggta
     4861 ttgcgattgg tattagcgtg atcctggcag tgatgatgta tcgctcgaag gccacgcaga
     4921 agctaaacaa tatcgcttac gcgctgatta ttggcggcgc gctgggcaac ctgttcgacc
     4981 gcctgtggca cggcttcgtt gtcgatatga tcgacttcta cgtcggcgac tggcacttcg
     5041 ccaccttcaa ccttgccgat actgccatct gtgtcggtgc ggcactgatt gtgctggaag
     5101 gttttttgcc ttctagagcg aaaaaacaat aataaaccct gccggatgcg atgctgacgc
     5161 atcttatccg gcctacagat tgctgcgaaa tcgtaggccg gataaggcgt ttacgccgca
     5221 tccggcaaaa atcctcaaat ataagagcaa acctgcatgt ctgaatctgt acagagcaat
     5281 agcgccgtcc tggtgcactt cacgctaaaa ctcgacgatg gcaccaccgc cgagtctacc
     5341 cgcaacaacg gtaaaccggc gctgttccgc ctgggtgatg cttctctttc tgaagggctg
     5401 gagcaacacc tgttggggct gaaagtgggc gataaaacca ccttctcgtt ggagccagat
     5461 gcggcgtttg gcgtgccgtc accggacctg attcagtact tttcccgccg tgaatttatg
     5521 gatgcaggcg agccagaaat tggcgcaatc atgcttttta ccgcaatgga tggcagtgag
     5581 atgcctggcg tgatccgcga aattaacggc gactccatta ccgttgattt caaccatccg
     5641 ctggccgggc agaccgttca ttttgatatt gaagtgctgg aaatcgatcc ggcattggag
     5701 gcgtaacatg cagatcctgt tggccaaccc gcgtggtttt tgtgccgggg tagaccgcgc
     5761 tatcagcatt gttgaaaacg cgctggccat ttacggcgca ccgatatatg tccgtcacga
     5821 agtggtacat aaccgctatg tggtcgatag cttgcgtgag cgtggggcta tctttattga
     5881 gcagattagc gaagtaccgg acggcgcgat cctgattttc tccgcacacg gtgtttctca
     5941 ggcggtacgt aacgaagcaa aaagccgcga tttgacggtg tttgatgcca cctgtccgct
     6001 ggtgaccaaa gtgcatatgg aagtcgcccg cgccagccgt cgtggcgaag agtcgattct
     6061 catcggccac gccgggcatc cggaagtgga aggtacaatg ggccagtaca gtaacccgga
     6121 agggggaatg tatctggtcg aatcgccgga cgatgtgtgg aaactgacgg tcaaaaacga
     6181 agagaagctc tcctttatga cccagaccac gctgtcggtg gatgacacgt ctgatgtgat
     6241 cgacgcgctg cgtaaacgct tcccgaaaat tgtcggtccg cgcaaagatg acatctgcta
     6301 cgccacgact aaccgtcagg aagcggtacg cgccctggca gaacaggcgg aagttgtgtt
     6361 ggtggtcggt tcgaaaaact cctccaactc caaccgtctg gcggagctgg cccagcgtat
     6421 gggcaaacgc gcgtttttga ttgacgatgc gaaagatatc caggaagagt gggtgaaaga
     6481 ggttaaatgc gtcggcgtga ctgcgggcgc atcggctccg gatattctgg tgcaaaatgt
     6541 ggtggcacgt ttgcagcagc tgggcggtgg tgaagccatt ccgctggaag gccgtgaaga
     6601 aaacattgtt ttcgaagtgc cgaaagagct gcgtgtcgat attcgtgaag tcgattaagt
     6661 cattagcagc ctaagttatg cgaaaatgcc ggtcttgtta ccggcatttt ttatggagaa
     6721 aacatgcgtt tacctatctt cctcgatact gaccccggca ttgacgatgc cgtcgccatt
     6781 gccgccgcga tttttgcccc cgaactcgac ctgcaactga tgaccaccgt cgcgggtaat
     6841 gtctcggttg agaaaaccac ccgcaacgcc ctgcaactgc tgcatttctg gaatgcggag
     6901 attccgctcg cccagggagc cgctgtgccg ctggtacgcg caccgcgtga tgcagcatct
     6961 gtgcacggcg aatcgggaat ggctggctac gactttgttg agcacaaccg aaagccaatc
     7021 gggataccag cgtttctggc gattcgtgat gccctgatgc gtgcaccaga gcctgttacc
     7081 ctggtggcca ttggcccgtt aaccaatatt gcgctgttac tttcacaatg cccggaatgt
     7141 aaaccgtata ttcgtcgtct ggtgatcatg ggcggttctt ctggtcgcgg caactgtaca
     7201 ccaaatgccg agtttaatat cgctgccgat ccagaagcgg ctgcctgtgt cttccgtagc
     7261 ggaatcgaaa tcgtcatgtg cggtctggat gtcaccaatc aggcaatatt aacccctgac
     7321 tatctcgcta ctttgccgga gttgaatcgc accggaaaaa tgcttcatgc actgtttagc
     7381 cactaccgta gcggcagtat gcaaagcggt ctgcgaatgc acgatctctg cgccatcgcc
     7441 tggctggtgc gcccggactt attcaccctc aagccgtgtt ttgtcgcggt agaaactcaa
     7501 ggcgaattta cctcaggcac gacggtggtt gatatcgacg gttgcctggg caaaccagcc
     7561 aatgtaaagg tggcattgga tctggatgtg aaaggcttcc agcagtgggt ggctgaggtg
     7621 ctggctctgg cgtcgtaacc tgtcacatgt tatcggcatg cagtcattca tcgactcatg
     7681 cctttcactg atatccctcc ctgtttatca ttaatttcta attatcagcg tttttggctg
     7741 gcggcgtagc gatgcgctgg ttactctgaa aacggtctat gcaaattaac aaaagagaat
     7801 agctatgcat gatgcaaaca tccgcgttgc catcgcggga gccggggggc gtatgggccg
     7861 ccagttgatt caggcggcgc tggcattaga aggcgtgcag ctgggcgcgg cgctggagcg
     7921 cgaaggatct tctttactgg gcagcgacgc cggtgagctg gctggtgcag ggaaaacagg
     7981 cgttaccgtg caaagcagcc tcgatgcggt aaaagatgat tttgatgtgt ttatcgattt
     8041 tacccgtccg gaaggtacgc tgaaccatct cgctttttgt cgccagcatg gcaaagggat
     8101 ggtgatcggc actacggggt ttgacgaagc cggtaaacag gcgattcgtg acgccgccgc
     8161 cgatattgcg attgtctttg ccgccaattt tagcgttggc gttaacgtca tgcttaagct
     8221 gctggagaaa gcagccaaag tgatgggtga ctacaccgat atcgaaatta ttgaagcaca
     8281 tcatagacat aaagttgatg cgccgtcagg caccgcactg gcaatgggag aggcgatcgc
     8341 ccacgccctt gataaagatc tgaaagattg cgcggtctac agtcgtgaag gccacaccgg
     8401 tgaacgtgtg cctggcacca ttggttttgc caccgtgcgt gcaggtgaca tcgttggtga
     8461 acataccgcg atgtttgccg atattggcga gcgtctggag atcacccata aggcgtccag
     8521 ccgtatgaca tttgctaacg gcgcggtaag atcggctttg tggttgagtg gtaaggaaaa
     8581 tggtcttttt gatatgcgag atgtacttaa tctcaataat ttgtaaccac aaaatat
//
LOCUS       DAEEKQ010000158         8635 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.160, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000158 DAEEKQ010000000
VERSION     DAEEKQ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8635
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.160"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="KLK57_003436"
     CDS             complement(144..2687)
                     /gene="mdoH"
                     /locus_tag="KLK57_003436"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HBE0893717.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="KLK57_003437"
     CDS             complement(2680..4215)
                     /gene="mdoG"
                     /locus_tag="KLK57_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HBE0893718.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            4610..5767
                     /gene="mdoC"
                     /locus_tag="KLK57_003438"
     CDS             4610..5767
                     /gene="mdoC"
                     /locus_tag="KLK57_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HBE0893719.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="KLK57_003439"
     CDS             complement(5775..7256)
                     /gene="clsC"
                     /locus_tag="KLK57_003439"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HBE0893720.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRQMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLSDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="KLK57_003440"
     CDS             complement(7198..7731)
                     /gene="ymdB"
                     /locus_tag="KLK57_003440"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HBE0893721.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(7826..8137)
                     /locus_tag="KLK57_003441"
     CDS             complement(7826..8137)
                     /locus_tag="KLK57_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0893722.1"
                     /translation="MFRPFLNSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="KLK57_003442"
     CDS             complement(8258..8590)
                     /gene="csgC"
                     /locus_tag="KLK57_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HBE0893723.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQ
                     WPPSSEKS"
BASE COUNT         2156 a         2248 c         2051 g         2180 t
ORIGIN      
        1 cataacgccg gtatttttat tggctcgttg tttttgccgg atgcgacgtg aacgctttac
       61 ctaacccaca aaagcttgca aaatcaataa attgcatgaa cgatgtaggc ctgataagcg
      121 tagcgcatca ggcaactaca tttttattgc gaagccgcat ccggtttacg caatgccagt
      181 ggattgagct ttatcccttc gtaataactc acccatgaag aatatctctc aggggaattc
      241 cagacgcgga aatgcagacg ggccatcgtc accggatcgc ttagcagcac aaggcgacga
      301 tcgcgattca gcttctctgg cgtctcgttc agcgcctgtt caacgtggcg gtcacgggcg
      361 atttccagca ccttactggc gcggtgacgc gcggtcgcca ttgcggttgc cagagcgtta
      421 aatgacgggt taaacactgc gtgcataaag ccatcgtcaa gggagcgttg gcgattcatc
      481 tcaaggaacc gatcggtatc aaccagcacc tgcggcggtg aatactcttc cgggatcagg
      541 aacagtttcc agcgtttggt gcgcagacca acggtggcac ggctggaaat caccgaaaca
      601 aacggtgaca ggatcaacga gaagacaatc ggtgccagcc agaacaggaa acgcagatcc
      661 agccacgcca tcccaacagc ccacactaac cctaacagca gctgtgagcc gtggcgtttg
      721 aacgcttcac cccaggaagt ggagtcatca tcacgctgcg gtgaattcca caccacttcc
      781 cagccaagga acgcgctgac gacgaagacc gtatggaaca gcatgcgtac cggagccagc
      841 agcacggaaa acagcacttc cagcagcaac gataatgtaa cgcgccagaa gccgccgtat
      901 tctttcgttc ctttgcacca gataagcaaa atgctcaata acttcggcag gaacaacagc
      961 accatggtcg aagcaaaaag tgcaatcgcc agctcaggac gccactgcgg ccacaccggg
     1021 aacaactgcc gtggttgcag gaagtattgc ggttcggtca acgcatgtac tacctgcaat
     1081 gcagtagaga gcgcgaggaa cataaaccac agcggagcgg agagataaga catcacgccc
     1141 gtcaggaaca ccgcacggtg aaccgggtgc atacccttca ccaggaacag acggaagttc
     1201 atcaggttac cgtggcacca tcggcggtca cgttttagct catcaagcaa gttaggcggc
     1261 aattcttcat aagaacccgg gagatcgtaa gcaatccaga ccccccaacc tgcacggcgc
     1321 atcaacgccg cttccacgaa gtcatgtgac aggattgaac cggcaaagga accttcgccc
     1381 ggcagcggag ccagtgcgca gtgctcgata aacggtttca cgcggataat cgcgttatgt
     1441 ccccagtagt gcgactcgcc aagttgccag aagtgcaaac cggctgtaaa cagtggccca
     1501 tacacgcggg tcgcgaactg ctgacagcgc gcatacagcg tatccatacc ggacgctttc
     1561 ggcgacgact gaatgatccc ggcgttcggg ttggcttcca tcaggcgcac cagcccgcac
     1621 aaacaatcac cggtcattac cgagtcagca tccagcacca ccatgtagct gtactggctg
     1681 ccccagcgac ggcagaagtc atcgatatta ccgcttttac gcttcacgcg gcgacggcgg
     1741 cggcgataga aaatctgacc ttcgccaccg acttcagcga taagctccat ccaggctttt
     1801 tgctctgcga cgcagatatc cgggttataa ctgtcactaa gaatgtagac atcaaagtgt
     1861 ttggcattcc cggtggcttt tactgattcc cacgttgcac gcaggccagc aaaaacacgg
     1921 ttcacgtctt cgttacagat aggcatgatc aacgccgtgc gatgctccgg gtttaatggt
     1981 tcatcgccaa ctgttgacgc agatatactg tatttatcgc gaccaataag cagttgcagg
     2041 aagcccatta acgccgtcca gaatccggcg gacacccaac agaacagtac cgcaaagagg
     2101 atcaggatac cggtttgcag catataaggc agaagctgca taaaggaaac ccacaaatcc
     2161 tgaccaacca tatccatagg attaatcagc gcccaaccct gataaggaag aatggtcttc
     2221 atataccagg tcgcgacgac agtttgcgcg agcgtcagga tcaacagaat gtaacggcgg
     2281 atggtaccga cggtacgcca cttttgctcg ctctcctgct cttctttggt caaacgagcc
     2341 agatagcgcg gcgtgacatc gcgtccacgc aggcgatccc agaaacggcc taccgggttg
     2401 gtacgccacg ggtcgggaaa catcgaggag cgttttgctt ctggcatcgc cttcagctga
     2461 tcgcgccctt cgtcgtcttt aattaactgt ccatcagcaa gtgaatctgg ccaggcttgt
     2521 tccagacgcg cctttaccga gccttgcggg gaatcatcct cccgcgccca ggtgcggtgt
     2581 tcggcatcca gcgcctgatg aacggcgcgg atatcagtct tcggcaatgc cgctttctcg
     2641 cttgcggcga tgggcattgc gtcaatgtac tcagttgtct tattcattgg caggtaactg
     2701 gtagctccag gtttcactca acgtctgatc ggcattcacc agcgcagcac gcatttcagt
     2761 ggttttcttg gcatctttca ctttcacacg catcaccaga cgccagcctt tggtaaccgg
     2821 gttatagcgc accgtgcttt caactatctc accattatca ccaatgctgg tttgcgctgt
     2881 gaccggggta tcctctggca gttttttcat ctctgcgccg gtaaaatcga ccacaaaggc
     2941 gatagtaccg tcaggctggc gaatcaggtt cgactgcttc acatcccccg ttgaacgacg
     3001 cgtttgttgc acccatgcgt tatctggcgc atgcagtttg tcttcatcac ggctgaaggt
     3061 gatggtgtat ttaaagttca tctctttacc cggctccggc agctgatccg gcgtccagta
     3121 agcgacgatg ttatcgttgg tttcatcgtt ggttggaatt tccaccagct caacgctgcc
     3181 tttaccccac tcccctttcg gagtcaccca tgcgcttgga cgaagatcgt aacgatcatc
     3241 gagatcttca aagcgggaga aatcacgacc gcgctgcaac agaccgaagc cttgcgggtt
     3301 ttccatcgag aagctgctaa ccgctaaatg tttcgggtta ttcaacggac gccagatcca
     3361 ttcgccatta ccggcatgga tagagagacc gttagagtcg tgcaactccg gacgatagtt
     3421 atttgcaggc gacggttggt tcggcccaaa caggaacata ctggttaacg gtgcaacccc
     3481 cagtttgccg actttatcgc gcagatagat tttcgactgc acatccacaa ccgtgtcacg
     3541 ccctggcata accacgaatt tgtaagcacc tgtcgcgcgc ggcgagtcaa gcaatgcata
     3601 aatggttaaa cgtttatcag tcggttttgg acgctcgatc cagaactctt tgaagcgtgg
     3661 aaattcttcc cccgatggca aggcggtatc aattgccagg ccgcgggcag aaaggccata
     3721 aacctgacct gcaccaatca cgcggaaata gctggccccg agcatgctga cgatttcatc
     3781 gtttttatct ttgctgttga tcgggtaaag cactttaaaa ccggcaaaac caaggtcttt
     3841 taccgtgtct ttgtcatgct gaacatcgcc gaaagtgaaa taatccgggc tgtatttgat
     3901 tcgtttgact gcggtggcag tcacttcatt tattttgacc ggggtatcga agtacatacc
     3961 ctgatggtag aactcgagtt tgaatggggt cttcagattg ttccagtacg ctttgtcatg
     4021 attaaactgg atctgctgat agtccgcgta tttcatatcg cggaaaacgg agggcaagtt
     4081 gcttttgggc gcctcatagc ctttcccggc taaggattga gcttgctttg cgacatcatc
     4141 aatactgaaa gcccagcttg aagatgtata cagggttaac attactgcag cactcaacca
     4201 acgcattttc atcatttgta gtttatgttt cataattagt aaagcacttc cccctttgtg
     4261 tgcttatatc gatccgatct attttaatgg ataaccagcc attccgacaa cagaatcccg
     4321 gtatcgttca gcacgctggc tcatggatta agcacctgat tgcaaccgtt ttcactttcc
     4381 gtgcttctcc taaagacaga agcatcgact tcgtgtaggg tttgccagca attttattta
     4441 tcaaccgtct tacagacgct tccattctgg ttagtggtag cgatgatgct atgcataaaa
     4501 accttgaagc ctgcgcctgg cgcggtcagg tcggtgtcgc tatggcaaca agcctaaagg
     4561 cataacccga cagggataag acgaaaaatc cgagattagt taaccatata tgaatccagt
     4621 acccgcgcaa cgtgaatatt tcctcgactc catccgcgcc tggctgatgt tgttagggat
     4681 cccttttcat atttctttaa tctattcgag ccatacatgg catgtgaata gcgccgaacc
     4741 gtcattgtgg ctgacccttt ttaatgactt catccactcg ttccgcatgc aggtattttt
     4801 cgttatatcc ggctactttt cctacatgct ttttttacgc tatcccttga aaaaatggtg
     4861 gaaagtacgt gtcgaacgtg taggtatccc gatgttaaca gccatcccct tactgacatt
     4921 gccgcaattt attatgctgc aatacgtcaa agggaaagcg gaaagttggc ctgggctgtc
     4981 attgtatgac aaatataata cgttggcctg ggaattaata tcacacctgt ggtttttact
     5041 ggtgttagta gtcatgacga cgctgtgcgt atggatattt aaacgcatca gaaataattt
     5101 agaaaattct gataaaacga ataaaaaatt ctcgatggta aaactatcgg tgattttttt
     5161 atgcctcggc atcggctatg cggtaataag aagaacgatt tttattgtgt atccgcccat
     5221 tctgagtaat ggcatgttca attttattgt catgcaaacg ctattttatt tgccgttctt
     5281 tatcctcggt gcactggctt tcattttccc tcatcttaaa gccttgttta ccacgccgtc
     5341 tcgtggctgt acccttgcag cagcattggc atttgtcgct tatttactca accagcgcta
     5401 tggcagtggc gatgcctgga tgtacgaaac cgagtcggtg atcaccatgg tcctcggtct
     5461 gtggatggtg aatgtggtct tctcctttgg ccaccgtttg cttaacttcc agtcagcgcg
     5521 ggtgacttac tttgttaacg catcgctatt tatctatctg gttcaccacc cgttaacgct
     5581 gtttttcggc gcatacatta caccgcacat cacctccaac tggcttggtt ttctctgtgg
     5641 cctgatattc gtagtaggga ttgcgataat tctgtatgaa attcatttac gcatcccgtt
     5701 actgaagttt ttgttttctg gtaaaccggt tgttaagcgt gagaacgata aagcaccagc
     5761 ccgttaagcc acatttacaa taaccattcc acgggcaata tcgacgccag tctgaccata
     5821 acccgcttcc agaaactggt ggcgggttct tttttgagaa taatctcttt ctttgcatga
     5881 cgatcaaccc agttgatccg tccccacctg tccagacgga gctgccaggc cgcatcatac
     5941 tggctctgaa taaagcgttt atcaattaac tgtgccagcg tttcgctctc tatcacgaag
     6001 cccatttcag tattgagcaa tgttgaacgc ggatcgaaat tgaaagagcc gataaacacc
     6061 gttttaccat cgatgctaaa agttttagca tgcaggctgg ctccggaatt accggttatg
     6121 ccgcgatcgt gtaacgtact actttgttca cgcgtcggct tgagttcata taattccacg
     6181 ccatagcgga gcaatttttt gcgccagcgc gcgtatccgg catggacgac agcaacatcg
     6241 ttagcggcaa gagaattggt taggatcgca atctttaccc cttttctcac catccgtaag
     6301 agttgcgcca cacctgcgcg tgtcggtaca aaataggaag agataatatc gatgcgttca
     6361 ctgggtgagc ccatgatatc ggacaggcgc tgcggtaaca gtgaatgccg ttttgccttg
     6421 ccctcccctt tcgccggatc atcacttaat aaacgtgttt tcgcccagat aagcggcaac
     6481 gttccatcaa ccagatgatt tataaacgga ctggattcca tttggcgtaa gtaacgatgc
     6541 gtcatggcat cgttatgcca ggaggcgggt aactcgatgc gatccgccat ttcaccttcc
     6601 gggacatcca gcacctgctg taagggtgaa accgatttgc aataccagta gcgggcgaaa
     6661 tcatcggcaa cgtcctctac cacgggtcct attgccataa catctaaatc cgaaaaaagt
     6721 ggctcttccc ctgccccaaa ataggcatca ccaatatttc gtcctcccac cagggtaacc
     6781 acgccatcga cagtgaaact tttattgtgc atacggcgat tgagacggga aaagtcggtg
     6841 atataaccaa gcggacgcag caagcgaaac gagaaaggat taaaaagccg gacttcaatg
     6901 cggggatggc tgtcaagcaa gcgtaaaata tcgtcaagtc cgggcgtatt gttgtcgtcc
     6961 agcaacaaac ggacgcgaac gccacgcttt gctgcggcta acagggcgga aaacagtaac
     7021 cgacccgaca tatcgtcctg ccagatgtaa tactgaacat ccagcgtatg ctctgccatt
     7081 tcggccagac gataccgggc ggcaaacgca tccagacttt tctccagagg gaaaagacca
     7141 cactgaccgg gatgttgcga acacagtggc agcaccgcgc tcgccagccg gggcaaatca
     7201 ttcatctcct tgttgggtaa ggagtctttc gtagaggtgg gcgttttctt catcataaca
     7261 gacaaagtat acctgttcgg gtaaagcgtg acgggtaata aattctgaaa cggttttaac
     7321 tgcgatttca gccgctgccg cacgagggta accataaacc ccagtactga ttgcaggaaa
     7381 agccactgac gtatagctgt ttgccgccac cagtcgcagg ctattcagat aggcatcctg
     7441 caaaagctgg tcttcgtttt gttcaccacc acgccagact ggcccgacgg tgtgtactac
     7501 ggctttagcg ggaagatcgc ctgcaagcgt aataacggca tgccccgtag ggcaatcgcc
     7561 ctgctgttgc ctgactttta aacaggcatc cagcagggcc ggacccgctg cgcgatgaat
     7621 ggccccatcg acgccgccgc ctcccattaa tgacggatta gccgcattca caatcacatc
     7681 aacggccagt ttggtaatat caccctgcac aacatgaata cgcgttttca taacgcctcc
     7741 ttacaccttc ttgtttctta agtgtacagc aggctttttg gttaaatcgc atttttctgc
     7801 tcagcaacgt gcgttgttca gaaacttagt tatattcgat gttcattaca gccagctttt
     7861 tctgtttatc aagatactgc acttttactg aggctatttg cgcgttttta acattaccgc
     7921 tcattaacgc tttgctggaa taggttttgc ccggaacaga accattctgt gggcagttca
     7981 tttctacggg tgatgactgg gtgctgacgt cacatgccgg ttccacaatt tggccataaa
     8041 aatggatcac acccccttgc gcggtgcttc ctgcgatggc aataaaagga aaaaacaaac
     8101 tgccgagcat aagagagttt aaaaaaggtc tgaacattta ttctatttcc tcaatgatta
     8161 gtcatccttg agggttgtgt tatccatact ttcaggttgg ctgattcgca gcagaccatt
     8221 ctctccagat tcatcttatg ctcgatattt caacaaatta agacttttct gaagagggcg
     8281 gccattgttg tgataaatga agtgcctgtc catcagaaac agtaacaact attttcaccc
     8341 gatcttccgg ggaaatattt aaactcaact tcgtcaaagc aataggttga ttagcaggca
     8401 atgagagggt cttttcttgc ttcgtctgac tttgccctga actgccttcg cgcagggaca
     8461 atatttgtac tctgcacaga caagattgag taagagtgac ttcaggaata atggtataca
     8521 catccccttg ctgggtcgta ttaaaggtta tctggctgga aagtgccgca aggagtaata
     8581 acgcattcat attcttctcc cgaaaaaaac agggcttgcg ccctgtttct gtaat
//
LOCUS       DAEEKQ010000159         8604 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.161, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000159 DAEEKQ010000000
VERSION     DAEEKQ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.161"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..161)
                     /locus_tag="KLK57_003443"
     CDS             complement(<1..161)
                     /locus_tag="KLK57_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBE0893724.1"
                     /translation="MNNNNTLYVGLDVHKESITVAYAINSEPVELMGKIGTSPTDIQN
                     LCKRLRSKSS"
     gene            288..482
                     /locus_tag="KLK57_003444"
                     /pseudo
     CDS             288..482
                     /locus_tag="KLK57_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            705..1202
                     /gene="ubiC"
                     /locus_tag="KLK57_003445"
     CDS             705..1202
                     /gene="ubiC"
                     /locus_tag="KLK57_003445"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HBE0893725.1"
                     /translation="MSHPALTQLRALRYFKEIPALDSQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1215..2087
                     /gene="ubiA"
                     /locus_tag="KLK57_003446"
     CDS             1215..2087
                     /gene="ubiA"
                     /locus_tag="KLK57_003446"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HBE0893726.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSILVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2242..4665)
                     /gene="plsB"
                     /locus_tag="KLK57_003447"
     CDS             complement(2242..4665)
                     /gene="plsB"
                     /locus_tag="KLK57_003447"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HBE0893727.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRPLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4836..5204
                     /gene="dgkA"
                     /locus_tag="KLK57_003448"
     CDS             4836..5204
                     /gene="dgkA"
                     /locus_tag="KLK57_003448"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HBE0893728.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5314..5922
                     /gene="lexA"
                     /locus_tag="KLK57_003449"
     CDS             5314..5922
                     /gene="lexA"
                     /locus_tag="KLK57_003449"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HBE0893729.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5995..7320
                     /gene="dinF"
                     /locus_tag="KLK57_003450"
     CDS             5995..7320
                     /gene="dinF"
                     /locus_tag="KLK57_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HBE0893730.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMSKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7436..7645
                     /locus_tag="KLK57_003451"
     CDS             7436..7645
                     /locus_tag="KLK57_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HBE0893731.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7687..8202)
                     /gene="zur"
                     /locus_tag="KLK57_003452"
     CDS             complement(7687..8202)
                     /gene="zur"
                     /locus_tag="KLK57_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HBE0893732.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1987 a         2080 c         2321 g         2216 t
ORIGIN      
        1 gatgactttg acctgagacg tttacaaaga ttctgaatat cagtaggtga tgtgccaatt
       61 ttacccatca gttcaacagg ttctgaattg atagcataag cgacagtaat cgattctttg
      121 tgaacatcta acccgacgta caatgtgtta ttgttgttca tgctggcctc cgtggtggaa
      181 ataccggggt gtagcaacag ctcacccatg cggctctgac aacgatgtta acccgcgtta
      241 aatatcccac gttggccagc atctgtctca cggaaagtca tactgcctta catgatatgt
      301 tgaatcctac aaaagatacc aattggaatt ccacgtgtat ctataaatcc agacataaga
      361 tgttgcctat taatcttact caggaaacac tttttagctc caaatctcat ggtaaatatg
      421 cgcttttccc aatttttact gcgagttggc gagctactcg tataaagaat ataggtattt
      481 aagtaaagga aaacatcacc gttcctggca tcatgggcgg tgatgcccct acggttgccc
      541 tcgccagcac gggcatcggc aaagcgtaag gttcaacagc gttttaccac ttcatgcgat
      601 tgttgcgttt ttgttgcgca ttagatcact taatttgctt tacatctccc gtaaacactt
      661 ttctgcgata caatgccttt acgttatgta acggagagtt cggcatgtca caccccgcgt
      721 taacgcaact gcgtgcgctg cgctatttta aagagatccc tgccctggat tcgcaactgc
      781 tcgactggct gttgctggaa gattccatga caaaacgttt tgaacagcag ggaaaaacgg
      841 tgagcgtgac gatgatccgc gaagggtttg tcgagcagaa tgaaatcccc gaagaactgc
      901 cgctgctgcc gaaagagtct cgttactggt tacgtgaaat tttgttatgt gccgatggtg
      961 aaccgtggct tgccggtcgt accgtcgttc ctgtgtcaac gttaagcggg ccggagctgg
     1021 cgttacaaaa attgggtaaa acgccgctag gacgctatct gttcacatca tcgacattaa
     1081 cccgggactt tattgagata ggccgtgatg ccggactgtg ggggcgacgt tcccgcctgc
     1141 gattaagcgg taaaccgctg ttgctaacag aactgttttt accggcgtca ccgttgtact
     1201 aagaggaaaa aaatatggag tggagtctga cgcagaataa gctgctggcg tttcatcgct
     1261 taatgcgtac ggataagcca attggcgcgt tactgctgct ctggccaaca ttatgggcgt
     1321 tgtgggtggc gacaccgggc gttccccagc tctggatcct ggcggtgttt gtcgcgggtg
     1381 tctggctgat gcgcgctgcc ggatgtgtgg tgaatgatta tgctgaccgc aagtttgatg
     1441 gtcatgttaa gcgcacggcg aaccgaccgc ttcccagcgg cgcggtaacg gagaaagagg
     1501 cgcgcgcgct gtttgtagtg ctggtattga tttcattttt actggtgctg acgctgaata
     1561 cgatgaccat tctgttgtcg attgccgctc tggcgttggc gtgggtgtac ccgtttatga
     1621 agcggtatac ccatctcccg caagtggtgc tgggcgcggc gtttggctgg tcgattccaa
     1681 tggcttttgc cgctgtgagt gagtcggtgc cattgagttg ctggttaatg ttcctcgcca
     1741 atattctctg ggcggtggct tacgacacgc agtatgcaat ggttgaccgt gacgatgatg
     1801 tgaagattgg tattaaatcc accgcaatcc tgttcggcca atacgataaa ttgattattg
     1861 gtattttgca gattggcgta ctggcactga tggcgatcat cggtgagtta aatggcttag
     1921 gctggggata ttactggtca attctggtgg ctggcgcgct gtttgtttat caacaaaaac
     1981 tgattgccaa ccgcgagcgt gaagcctgct ttaaagcatt tatgaataat aactatgttg
     2041 gtctggtact gtttttagga ctggcaatga gttactggca tttctgatga tgtaaaaaag
     2101 ccggatgatc atccggcttt cttctgggtt gcctgatgcg ctgcgcttct caggcctaca
     2161 caacacatcg caatttattg aatttgcaga ttatggaagg ccggataagg cgttttcgcc
     2221 gcatccggca attctctctg attacccttc gccctgcgtc gcactctcaa tcgtcaaacg
     2281 cacgtctgat gtaattaact ccgccagcaa ctgataaacc ttcatcgttt ctgccggttc
     2341 ggcgtcgccg ctatcgctga tatacccttc atcacgcagc gtcagcacca gagaactgaa
     2401 caccgccttg tcgaagaact ccggcgcgtt gatgccgtgc agcacggaga gacgttgcgc
     2461 aacggtgcgg ctctctttct ccagcgtacc gcggttgatc gacgggttgg cactcaacaa
     2521 ccagaaggtg atggcataac gttgcagcgt ttcgcgcgcg cctgcggcca gcagctgtag
     2581 ggggcgagaa tgcgccgggt tgatatgcaa ctcatcatct tgcagggtaa tcagcccctg
     2641 acgttgcatc tcatttgcca gcgcatcaat aacgtccggc aactcgtcgc gatcccagcg
     2701 caggaacagc tccgctttca gcattgggta aagcacattg acgtgctcca tcaatacgtc
     2761 gcgggagatg tggcgatgct gggtgacaat tgccgccatc agcgaaggca gcaccaacat
     2821 atgcgcaatg ttgttgcgat agtaggtcat cagcaccgct tgctcgcgcg gcagaatgat
     2881 gatgtcgccg atagtgtctt tctcgacttc aaacttgttc atttgcagcg cgtgatcgat
     2941 aagctcgctg gcgctggctg aaggaacggt agagtccgtg gagtaaggta cgttgcgcat
     3001 cagatccagg tagcagttga gttgctcggt taactgctcg cgggtgagtg agcgctgacg
     3061 tgatgccaga agcgcagtac aacacaggtt catggcgttt gccgcgcctg cgttgttaat
     3121 gcgtaccatc agatcggcag caatattatt gaccgtcggc gttaaccagg ccggacgcac
     3181 cgcttcgatg ggatcgatag attcacgcca gtctggtacg tgctggttaa ggtaggtcat
     3241 caacggcatt ggttcaccga agttgacgta accctgaccg agattacgca gcttgcttaa
     3301 accgcgcagc atctgcggca ggctctcttt ctctttcgtc gcgccgcgca gttctttggc
     3361 gtaagtaccc acttccatga cgtgctcata accgatatag atcggaatca gcgtaatcgg
     3421 acgcgtgccg ccacgcagca tcgcctgaat ggtcatcgac agcgtaccgg ttttcggatc
     3481 cagcaaacgc cccgtgcggg aacgaccgcc ttccacgaag tactcgacgg aataaccacg
     3541 gctgaacagt tcgccgagat actcacggaa aacggtggaa taaagtttat tgcctttaaa
     3601 agtacggcga ataaagaacg cccccagacg gcggaaaatc ggcccggccg gccagaagtt
     3661 caggttgatc ccggcggcga tatgcggcgg caccagcccc tggtggtaca gcacgtaaga
     3721 aagtagcagg tagtccatgt gactgcggtg gcaaggcaca tacaccagct catgaccgtc
     3781 gtgggccagc tggcgaacgc gctcggcgtt atggacgttg atgccctgat aaagtcggtt
     3841 ccaggtgaag cccagaatgc ggtcagtcag gcggatcatc tcgtaagaga aattcgccgc
     3901 aatctcttcc atcagcgcaa tcgcgttctg ctgcgctttt tcatgggaga tttttttgct
     3961 gcgcgcttca tcttctaccg ctttggcaat ggcgcgggag gcgagcagct tattaaacag
     4021 atcctgacga gcaggcagac gtgggcctac ggcagccaga cgttgacggg caaagtgcat
     4081 acgcgccacg cgcgccagtt tctgtgcgat agttttatcc gtgccgtgtt catccgccat
     4141 acggcgcagc gaaactgacg gcgagaaacg cacaaaactg tcgcgaccga gccacagtac
     4201 ggcgaaaaat ttctgtacgc cgttaagcat acgcagcggc gggtttactt cgcctttttc
     4261 acgccccggc gcgcgaccaa acatcaccga tactggcacc atctgcacat ccagatttgg
     4321 gttgctacgg tgcaaatcga gatagtcgtg gaacagctta atagactctt ctttcggcgt
     4381 gtaataggtg aatacacgcg gcccgccgtg aatgaacaca tagcgcggca gtagcgtgcc
     4441 gtcgatttct agcggctcta acgggtcagg caagtcatga gccagacact gggcgcgtaa
     4501 cgtcagcaaa tctgctttcg agttgtacgg taaaacgtac ataattggac gagaggtatc
     4561 cagccccagt tccggggcag gatctgccgg aatagacttg ctttttacca ggatgcttaa
     4621 tggtaaattc agtaatttgt agtaaattcg tggccagccg gacataaacg atgtaaagcc
     4681 tctggttaat aatgcaaatg cgcggcaagg atagcagaaa gtcatgggaa attctgtggt
     4741 atccgctcat gtttcgcgcg gcgttacgca aacccgaatc atcggattta acggtacact
     4801 gatattgacg ctcataatgt aaaaaggttc tttcaatggc caataatacc actggattca
     4861 cccgaattat caaagctgct ggctattcct ggaaaggttt acgcgctgca tggatcaacg
     4921 aagcggcatt ccgtcaggaa ggcgtagcgg tattgttggc ggtggtcatc gcctgctggc
     4981 tggatgtgga cgcaattacc cgcgtgctgc ttatcagctc cgtgatgctg gtgatgattg
     5041 tggaaattct caatagcgcc atcgaagcag tggttgaccg aattggctct gaataccatg
     5101 agctttccgg acgcgctaaa gatatgggat ccgccgcggt gctgattgcg attatcgtcg
     5161 ccgtgataac ctggtgcatc ctgttatggt cgcattttgg ataacccttc cagatttcga
     5221 taaatctctg gtttattgtg cagtttatgg ttccaaaatc gccttttgct gtatatactc
     5281 acagcataac tgtatataca cccagggggc ggaatgaaag cgttaacggc caggcaacaa
     5341 gaggtgtttg atctcatccg tgatcacatc agccagacag gtatgccgcc gacgcgtgcg
     5401 gaaatcgcgc agcgtttggg gttccgttcc ccaaacgcgg ctgaagaaca tctgaaggcg
     5461 ctggcacgca aaggcgttat tgaaattgtt tccggcgcat cacgcgggat tcgtctgttg
     5521 caggaagagg aagaagggtt gccgctggta ggtcgtgtgg ctgccggtga accgcttctg
     5581 gcgcagcagc atattgaagg tcattatcag gtcgatcctt ccttgttcaa gccgaatgct
     5641 gatttcctgc tgcgcgtcag cgggatgtcg atgaaagata tcggcattat ggatggtgac
     5701 ttgctggcag tgcataaaac tcaggatgta cgtaacggtc aggtcgttgt cgcacgtatt
     5761 gatgacgaag ttaccgttaa gcgcctgaaa aaacagggta ataaagtcga actgttgcca
     5821 gaaaatagcg agtttaaacc aattgtcgtt gaccttcgtc agcagagctt caccattgaa
     5881 gggctggcgg ttggggttat tcgcaacggc gactggctgt aacatatctc tgagaccgcg
     5941 atgccgccag gcgtcgcggt ttgtttttca tctctcttca tcaggcttgt ctgcatggca
     6001 ttcctcactt catctgataa agcactctgg catctcgcct tacccatgat tttctccaat
     6061 atcaccgttc cgttgctggg actggtcgat acggcggtaa ttggtcatct tgatagcccg
     6121 gtttatttgg gcggcgtggc ggttggtgca acggcgacca gctttctctt tatgctgttg
     6181 ctgtttttac gcatgagcac caccgggctg actgcgcagg cttatggtgc caaaaatcct
     6241 caggcattag cccgtgcgct ggtgcaaccg ttgctgttgg cgttgggggc tggggcgtta
     6301 attgcgctgc tgcgtacacc gattatcgat ctggctctgc atattgttgg cggtagtgaa
     6361 gcagtcctgg aacaggcgcg gcgctttctt gaaatccgct ggttaagcgc accggcgtcg
     6421 ctggcgaatc tggtattact cggttggtta ctcggcgtgc aatatgcccg tgcgccagta
     6481 attttgttag tggtcggcaa tatcctcaac attgtgctgg atgtctggct agtgatgggg
     6541 ctgcatatga acgtgcaggg cgcggctctg gcgacggtta ttgcggaata tgcaacattg
     6601 ctgattggtc tgctaatggt gcgtaaaatc ctcaaactac gcggaatttc cggcgaaatg
     6661 tcgaaaactg cctggcgagg aaacttccgt cgcttgctgg cgcttaaccg cgatatcatg
     6721 ctgcgttcgc tgttgttgca actctgtttc ggcgcgatca ccgtacttgg cgcgcgactg
     6781 gggagtgaca ttatcgctgt aaacgcggtt ctgatgacgc tactcacctt taccgcctat
     6841 gcgctggatg gttttgccta cgcggttgaa gcacattccg gtcaggcata cggtgcgcgc
     6901 gacggtagcc agttgctgga tgtctggcgg gcagcgtgcc gccagtcggg gatcgtagcg
     6961 ttactatttt cggtggttta tttgctggca ggggaacaca tcattgcgtt gctgacgtcg
     7021 ttaacccaga ttcagcagct ggctgaccgc tatcttatct ggcaggtgat tttgccgttg
     7081 gttggcgtct ggtgttatct gctggacggc atgtttatag gcgcaacgcg tgccgccgaa
     7141 atgcgtaaca gtatggcggt ggccgccgca ggttttgcgc tgacgctcct tacgctgccg
     7201 tggctgggta atcatggttt gtggctggca ctaaccgtct ttctggcgtt gcgcgggctt
     7261 tctctggcgg ctatctggcg gcgtcactgg cgtaacggta cctggtttgc cgcaacgtga
     7321 cggttaaaaa ttctgaataa attatcctaa gccaaattgc tgactacact taatctcacg
     7381 ttcagaagaa aagtgaacgt actctcattc acaacctaac gatgaggtct tgattatgaa
     7441 taaagatgaa gccggcggta actggaaaca gtttaaaggt aaagtgaaag agcaatgggg
     7501 caaactgacc gatgatgata tgacgatcat tgaaggtaaa cgtgatcaac tggtcggtaa
     7561 aatccaggaa cgttatggtt atcagaaaga tcaggcagaa aaagaggtcg tggattggga
     7621 aacccgcaat gaatatcgct ggtaattaat ccctcctgcc cgacgtgtac aaggatgtac
     7681 gccctcttaa cgcggtttct ttttcacctg cacagagtga tcatgctggc actgttcagg
     7741 atgacgacac gcttccactt ccacgcatgc cgcacagagc ccatgtgctt caatcacatt
     7801 atgccgcagg gcaaacccca tttttgccgc cagcgtatgc ataatgtctt ccacgccttc
     7861 tgcacactct tctttcaccg cgccgcagcg atcgcatata aacatggctg acgtatgcgt
     7921 gggctggtcg aacagatgac agagcacata actgttggtg gattccacct tatgcacaaa
     7981 accttgctca agcagaaaat ccagcgcgcg ataaaccgtt ggcggcttgg cttgcggttc
     8041 agcttcgcgc agtaaatcaa gcagatcata agcgctgata gcgccatcct gcagactcat
     8101 caggcgcaac acttccaggc gctgtggggt caggcgcaca ttacgctgcg cgcagatttt
     8161 ttcagcctgc gctaataact cctgcgttgt ggtcttttcc atcgggcacc tcaaagtggg
     8221 ggttaagaat gtccttactt taccatgttc caggaaaaac accgagaacc cttattgttg
     8281 ccgtaatgtt gattttctgt tttgtaggta aggtgttatg ttgccttgtc gtaccattat
     8341 caacacgata ataattaata attcattttt aaataaattg cttcaagtta actcgctatt
     8401 tatttaactg ggtttaaacg catacctgta gttcagaaat tatgagcgat ttttgatagt
     8461 gaccagatgg caaaagaatt ataaggagga cacggtacga caaaaaaggt agatgaatca
     8521 tgttgaagat aatacccggt gcaactggat actttaataa aacactcaat tcaaatcaat
     8581 tcgataatga agatgcaatc aaag
//
LOCUS       DAEEKQ010000160         8591 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.162, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000160 DAEEKQ010000000
VERSION     DAEEKQ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8591
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.162"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(170..1834)
                     /gene="glnS"
                     /locus_tag="KLK57_003453"
     CDS             complement(170..1834)
                     /gene="glnS"
                     /locus_tag="KLK57_003453"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HBE0893733.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(2093..4039)
                     /gene="nagE"
                     /locus_tag="KLK57_003454"
     CDS             complement(2093..4039)
                     /gene="nagE"
                     /locus_tag="KLK57_003454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HBE0893734.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDAMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVSDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGNVVAGQTPLYEIKK"
     gene            4372..5172
                     /gene="nagB"
                     /locus_tag="KLK57_003455"
     CDS             4372..5172
                     /gene="nagB"
                     /locus_tag="KLK57_003455"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBE0893735.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5232..6380
                     /gene="nagA"
                     /locus_tag="KLK57_003456"
     CDS             5232..6380
                     /gene="nagA"
                     /locus_tag="KLK57_003456"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HBE0893736.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLARHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTSFTPDFKITKTIVNGNEVVTQ"
     gene            6389..7609
                     /gene="nagC"
                     /locus_tag="KLK57_003457"
                     /gene_synonym="nagR"
     CDS             6389..7609
                     /gene="nagC"
                     /locus_tag="KLK57_003457"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HBE0893737.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLSSEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7657..8409
                     /gene="nagD"
                     /locus_tag="KLK57_003458"
     CDS             7657..8409
                     /gene="nagD"
                     /locus_tag="KLK57_003458"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HBE0893738.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
BASE COUNT         2101 a         2268 c         2187 g         2035 t
ORIGIN      
        1 cggcgtgaac gccttaaccg gcctacaaaa gcacgcaaac tcaatatatt gcagagatca
       61 tgtaggcctg ataagcgtag tgcatcaggc aatttagcgt ttgaaactgt ttgtctgttc
      121 cttgccggat gcgacgtaaa cgccccatcc ggcatagcga aacttaaaat tactcgccta
      181 ctttcgccca ggtatcacgc agcccaacgg tgcggttaaa taccggtttt tccgccgtag
      241 aatggcggct atcgaggcag aagtaacctt cacgctcaaa ctggaatgct ttacccgcaa
      301 ccgcatcttt cagcgacggt tcagcaaagc cctgtttgat caccagcgat tccgggttaa
      361 tcaccgacag gaaatcatcc gcagcaccag ggttcggcac gctgaacaga cggtcgtaca
      421 gacggatttc aaccggcagc gcatgtgccg cgctcaccca gtgaataaca cctttgactt
      481 tacgaccatc tgccggatct ttgcttaagg tgtcggcgtc ataagtacag aagatggtgg
      541 tgatattgcc ttcggcatct ttctccacgc gttctgcctt aatgacgtaa gcattacgca
      601 gacgcacttc tttacccagc accagacgtt tgtactgctt gttagcttct tcgcggaaat
      661 cggcgcgatc gatccaaatc tcaccgctaa acggcacctg gcggctaccc atttccggtt
      721 tgttcggatg gttcggcatg gtaaccattt cgccttcgcc ctgatagttt tcgataacca
      781 gtttcaccgg atcgataacc gccattgcgc gcggggcgtt ttcgttgaga tcttcacgga
      841 tgcaggattc cagcgacgcc atctcaatgg tgttgtcctg cttggtcacg ccgatgcgtt
      901 tgcagaactc acgaatagaa gcagcagtgt aaccacgacg acgcagaccg gaaatggtcg
      961 gcatacgcgg gtcatcccag ccttcaacgt gcttgtcggt caccagcagg ttcagcttac
     1021 gcttggacat cacagtgtat tccagattca ggcgcgagaa ttcgtactgg cgcgggtgaa
     1081 caggaatggt gatgttgtcc agcacccagt cgtacaggcg acggttgtcc tggaactcaa
     1141 gcgtacacag agagtgcgta ataccttcca gggcatcgct gatgcagtgg gtgaagtcgt
     1201 acatcgggta gatgcaccac ttgttgccag tctggtggtg ttcagcaaat ttaatgcggt
     1261 acagcaccgg atcgcgcatc acgataaacg gtgaagccat gtcgattttc gcacgcaggc
     1321 aggctttacc ttcttcaaaa ccaccggcac gcattttttc gaacagcgcc aggttctctt
     1381 caacgctgcg gtcgcggtac gggctgtttt tacccggttg cgtcagggtg ccgcggtatt
     1441 cgcggatctg ttccggcgtc agttcatcaa cgtacgccag gcctttattg atcagttcga
     1501 tcgcataggc gtggagctga tcaaaataat cggaggagta acggacgtta ccagaccagt
     1561 gaaaacctaa ccactctacg tcgtttttga tcgactcaac atactcgata tcttctttta
     1621 ccgggttagt gtcgtcgaaa cgcaggttgc actggccttt atagtcctgg gcgatcccga
     1681 agttcaggca gatagatttc gcatggccaa tatgcagata gccattcggc tccggcggga
     1741 aacgggtgtg tactgtggtg tgcttaccac tggccagatc ttcatcgatg atctgacgga
     1801 taaagttagt cgggcgggct tctgcctcac tcatcgtgga ttcctcaaag cgtaaacaac
     1861 gtataacggc gtatgatctt ataagcggga caggctgaca actgttagtt agcgaaaatc
     1921 gaaaatctca ctgagattat ggggatgatt gatgaaaaaa aaagcggcag agaagatctc
     1981 caccgctttt gccagtcccg cgtcattgtt ggatgcgcgt cttatccaac caggactgag
     2041 cttttgtagg cctgataaga cgcgtcaagc gtcgcatcag gcataaagca gattactttt
     2101 tgatttcata cagcggtgtt tgacccgcca caacattgcc ctgagcttta atgatcaagc
     2161 cactgaaatc gtcgatattg ctgcaaacca ccgggctaat catcgaacgg gcgttagcgt
     2221 tcaggtaatc cagatccatt tccagaatcg gttgccctgc gctcacctgc gcgccctctt
     2281 ccaccagacg tttaaagcct ttaccttcca gcgctacggt gtcgataccc atgtggacga
     2341 caatctccgc gcctttttcg gtttctaggc agaacgcgtg gttggtgttg aagattttca
     2401 cgattgtccc tgcggctggt gatacgacga ttttatctgt cggtttcacc gccacaccgt
     2461 cacccaccgc tttgctggcg aatgcttcgt cagaaacctg atccagtgcc acgacatcac
     2521 cggtaatcgg cgataccagc tccgcgatag ataccgcgtt tggtacagcc tgcggttttg
     2581 ctacaggagc ggcagttgcc ggagttgctt cagctgacgc agctgctacc gggccacggg
     2641 caacgacttt cttcatcgca tcgccaatgg attctgcttt cgcgccaaca atcacctgaa
     2701 tggtctgttt gttcagtttt actacaccag aagcacccag acgtttacac atcgcatcgt
     2761 tgacgcgagc tgagtcagcc acggtaaggc gcagacgggt aatacaggcg tcaatcgctt
     2821 tcaggttgtc agtaccgcca accgcagcaa tatagttggt tgccagttga gtcagacctt
     2881 cttcagtatt gctgttagcc tcttcagtta cgatctcgtc ttctttatct tcacgacccg
     2941 gcgttttcag gttgaacatg cggataacca aactgaacac cacgaagtag atagcgaaga
     3001 agacaacacc catcaccagc agcatccaga cgttctggct ggcggccggc aggttataca
     3061 tcaacgcgta gtcgatagcc cccgcagaga aagagaagcc agcatggata cccagcagcg
     3121 ttgccacaaa caggctgata ccggtcagca gtgcgtgcag gaggtacagc agcggagcaa
     3181 ggaacatgaa caggaattcc agcggctcag tcacaccggt caggaacgca gtaacagcaa
     3241 cagaaagcag catcccgcca accatcggac gacgctcttt cggtgctgcg aagtacatcg
     3301 ccagcgccgc acccggcaga ccgaacatca tgatcgggaa gaagccggac atgaacatcc
     3361 ccgcggtgcc gtcaccagcg tagaaacggt tgatgtcgcc gtggaaaacc gtacccgccg
     3421 cgttggtgaa ttcaccaatc tggaaccagg cgatggtgtt cagcacctga tgcagaccgg
     3481 ttgggatcag cagacggttg atgaaaccaa agataccgga acccagcgcg cctgcagaaa
     3541 cgatccactc gccgcctgca tggatagcgt gctgtactgg cggccagacg taaccaaaaa
     3601 tggccgccag caccaggcag aaaaagccgg tggcaatcgg cacaaagcgt ttgccgccga
     3661 agaagctcag gaagtccggc agtttaatat cggaccaacg gttataggct gcgccaccaa
     3721 ccagaccggt aatgataccc gccagtacac ccatgttaat ttctgggttg atggtcacca
     3781 tcgctttggt taacacaaag taacctaccg cacccgccag cgccgccgca cctgcgctgt
     3841 ctttcgacca gctggatgcc acaccgatgg cgaagattaa tgcgaggtta tcaaaaatcg
     3901 caccgcccgc ctgggcaata aacgcaacgt taagtaaatc tggctgaccg aatcgcagca
     3961 acagtgccgc caccggcagc accgcgatag ggagctgtaa cgccctaccg agtcgctgga
     4021 aaaaacctaa aatattcatc ttattccccc tacgagaacc ctatttggct cgtttaaagc
     4081 cgtattttta ttttgctgca aattgtactg ccgatgttct gtaatcagat tgttagatca
     4141 tctgctacag agtgtgtgaa aatttaattc gtatcgcaaa ttaaacgcgt gccttttgtg
     4201 agttttgtca ccaaatatcg ttatcatcac tcccttttac tggctaaagc agaaaactta
     4261 ttttatcatt caaaaaatca ggtcggattg acgcctgtct gcgcaaatcc aggttacgct
     4321 taaagatgcc taatccgcca acggcttaca ttttacttat tgaggtgaat aatgagactg
     4381 atccccctaa ctaccgctga acaggtcggc aaatgggctg ctcgccatat cgtcaatcgt
     4441 atcaatgcgt tcaaaccgac tgccgatcgt ccgtttgttc tgggcctgcc gactggcggc
     4501 acgccgatga ccacctataa agcgttagtc gaaatgcata aagcaggcca ggtcagcttt
     4561 aagcacgttg tcaccttcaa catggacgaa tatgtcggtc tgccgaaaga gcatccggaa
     4621 agctactaca gctttatgca ccgtaatttc ttcgatcacg ttgatattcc agcagaaaac
     4681 atcaaccttc tcaacggcaa cgccccggat atcgacgccg aatgccgcca gtatgaagaa
     4741 aaaatccgtt cttacggaaa aattcatctg tttatgggcg gtgtaggtaa cgacggtcat
     4801 attgcattta acgaaccggc gtcttctctg gcttctcgta ctcgtatcaa aaccctgact
     4861 catgacactc gcgtcgcgaa ctctcgtttc tttgataacg atgtcaatca ggtgccaaaa
     4921 tatgccctga ctgtcggtgt tggtacattg ctggatgccg aagaagtgat gattctggtg
     4981 ctgggtagcc agaaagcact ggcactgcag gctgcagttg aaggttgcgt gaaccacatg
     5041 tggaccatca gctgtctgca actgcatcca aaagcgatca tggtgtgcga tgaaccttcc
     5101 accatggagc tgaaagttaa gactttaaga tatttcaatg aattagaagc agaaaatatc
     5161 aaaggtctgt aattgttatc cctgccctgg ctccttgctc agggcaatat tttttaaaat
     5221 cggggggcag aatgtatgca ttaacccagg gccggatctt taccggccac gaatttcttg
     5281 atgaccacgc ggttgttatc gctgatggcc tgattaaaag cgtctgtccg gtagcggaac
     5341 tgccgccaga gatcgaacaa cgttcactga acggggccat tctctccccc ggttttatcg
     5401 atgtgcagtt aaacggctgc ggcggcgtac agtttaacga caccgctgaa gcggtcagcg
     5461 tggaaacgct ggaaatcatg cagaaagcca atgagaaatc aggctgtact aactatctgc
     5521 cgacgcttat caccaccagc gatgagctga tgaaacaggg cgtgcgcgtt atgcgcgagt
     5581 acctggcaag acatccgaat caggcgttag gtctgcatct ggaaggtccg tggctgaatc
     5641 tggtaaaaaa aggcacccat aatccgaatt ttgtgcgtaa gcctgatgcc gcgctggtcg
     5701 atttcctgtg tgaaaacgcc gacgtcatta ccaaagtgac cctggcaccg gaaatggttc
     5761 ctgcggaagt catcagcaaa ctggcaaatg ccgggattgt ggtttctgcc ggtcactcca
     5821 acgcgacgtt gaaagaagcg aaagccggtt tccgcgcggg gattaccttt gccacccatc
     5881 tgtacaacgc gatgccgtat attaccggtc gtgaaccggg cctggcgggc gcgatcctcg
     5941 acgaagctga catttattgc ggtattatcg ctgatggcct gcatgttgat tacgccaaca
     6001 ttcgtaacgc taaacgcctt aaaggcgaca aactgtgtct ggttaccgac gccaccgcgc
     6061 cagcaggtgc caacattgaa cagttcattt ttgcgggtaa aacaatatac taccgtaacg
     6121 gactttgtgt ggatgagaac ggtacgttaa gcggttcatc cttaaccatg attgaaggcg
     6181 tgcgtaatct ggtcgaacat tgcggtatcg cactggatga agtgctgcgt atggcgacgc
     6241 tctatccggc gcgtgcgatt ggcgttgaga aacgtctcgg cacactcgcc gcaggtaaag
     6301 tagccaacct gacctcattc acacctgatt ttaaaatcac caagaccatc gttaacggta
     6361 acgaggtcgt aactcaataa gagaaagtat gacaccaggc ggacaagctc agataggtaa
     6421 tgttgatctc gtaaaacagc ttaacagcgc ggcggtttat cgcctgattg accagtacgg
     6481 gccaatctcg cggattcaga ttgccgagca aagccagctt gcccccgcca gcgtaaccaa
     6541 aattacgcgt cagcttatcg aacgcgggct gatcaaagaa gttgatcagc aggcctccac
     6601 cgggggccgc cgcgctatct ccatcgtcac cgaaacccgc aatttccacg caatcggcgt
     6661 acggcttggt cgtcacgacg ccaccatcac cctgtttgat ctcagcagca aagtgctggc
     6721 agaagaacat tacccgctgc cggaacgtac ccagcaaacg ctggaacatg ccctgttgaa
     6781 tgccattgct cagtttattg atagctacca gcgcaaactg cgcgagctga tcgcgatttc
     6841 ggtgatcctg ccagggcttg ttgacccgga cagcggcaaa attcattaca tgccgcatat
     6901 tcaggtagaa aactgggggc tggtagaagc tctggaagaa cgttttaaag tgacctgttt
     6961 cgttggtcac gatatccgta gtctggcgct ggcggagcac tacttcggtg caagtcagga
     7021 ttgcgaagac tccattctgg tgcgtgtcca tcgcggaacc ggggccggga ttatctctaa
     7081 cgggcgcatt tttattggcc gcaacggtaa cgtcggtgaa attggccata ttcaggtcga
     7141 accgctgggt gaacgctgcc actgcggcaa ctttggctgc ctggaaacta tcgccgccaa
     7201 cgctgccatt gaacaacggg tgttgaatct gttaaagcag ggctaccaga gccgcgtgcc
     7261 gctggacgac tgcaccatca aaactatctg caaagccgcg aacaaaggcg atagtctgtc
     7321 gtcggaagta attgagtatg tcggtcgtca tctgggtaaa accatcgcca ttgctatcaa
     7381 cttatttaat ccgcaaaaaa ttgttattgc cggtgaaatc accgaagccg ataaagtgct
     7441 gctccctgct attgaaagct gcattaatac ccaggcgctg aaggcgtttc gtactaatct
     7501 gccggtggta cgttctgagc tggatcaccg ctcggcaatc ggcgcttttg cgctggtaaa
     7561 acgcgccatg ctcaacggta ttttgctcca gcatttgctg gaaaattaat gtgcttttat
     7621 agtggcgctt attgttgtca atattctggg tagtccatga ccattaaaaa tgtaatttgc
     7681 gatatcgacg gcgtgctgat gcacgataac gtcgccgtac cgggtgcagc ggaatttttg
     7741 cacgggatta tggataaagg cctgccgctg gtgttgctga ccaactatcc ttcgcagacc
     7801 gggcaagatc tggcgaaccg ctttgccact gccggtgtcg atgtgccgga tagcgtgttt
     7861 tatacctctg cgatggcgac cgccgatttc ctgcgtcgcc aggaaggcaa gaaagcatat
     7921 gtggtgggcg aaggcgcact gattcatgaa ctgtacaaag ccggtttcac tattaccgat
     7981 gtgaaccctg atttcgtgat tgttggcgaa acgcgttcct acaactggga catgatgcat
     8041 aaagcggcct atttcgtcgc taacggtgca cgctttatcg ccaccaaccc ggacacccac
     8101 gggcgcggtt tttatcccgc ttgtggcgcg ttgtgtgcag ggattgagaa aatctccggg
     8161 cgcaaaccgt tctatgttgg taagcccagc ccgtggatca tccgcgcagc attaaacaaa
     8221 atgcaggcgc attcggaaga aacggtgatt gtcggcgata acttgcgcac cgacattctg
     8281 gcgggcttcc aggcaggtct ggaaacgatt ctggtgcttt ctggtgtttc atcgctcgac
     8341 gatatcgaca gtatgccgtt ccgcccaagc tggatttacc cgtcagtcgc tgaaatcgac
     8401 gttatctgaa taccacccac gtctgcggac gtggttggct ctgatgacaa acgccaaact
     8461 gcctgatgcg ctacgcttat caggcctacg cagctcctgc aatatattga attttcatgc
     8521 ttttgtaggc cggataaggc gttcacgccg catccggcat gaacaaatcg cactttatca
     8581 gcaatttggc c
//
LOCUS       DAEEKQ010000161         8533 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.163, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000161 DAEEKQ010000000
VERSION     DAEEKQ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.163"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="KLK57_003459"
     CDS             complement(<1..358)
                     /gene="mhpC"
                     /locus_tag="KLK57_003459"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308431.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HBE0893739.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHS"
     gene            complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="KLK57_003460"
     CDS             complement(376..1320)
                     /gene="mhpB"
                     /locus_tag="KLK57_003460"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HBE0893740.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISAFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(1322..2986)
                     /locus_tag="KLK57_003461"
     CDS             complement(1322..2986)
                     /locus_tag="KLK57_003461"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HBE0893741.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFSRELEAFSQQDDEVTLHLKTAEG
                     QRETIKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALVREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGNTLNKLASVMTLTRPDADVSVEKVA"
     gene            3028..3861
                     /gene="mhpR"
                     /locus_tag="KLK57_003462"
                     /pseudo
     CDS             3028..3861
                     /gene="mhpR"
                     /locus_tag="KLK57_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
     gene            3938..5020
                     /gene="lacI"
                     /locus_tag="KLK57_003463"
     CDS             3938..5020
                     /gene="lacI"
                     /locus_tag="KLK57_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HBE0893742.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            5143..8217
                     /gene="lacZ"
                     /locus_tag="KLK57_003464"
     CDS             5143..8217
                     /gene="lacZ"
                     /locus_tag="KLK57_003464"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HBE0893743.1"
                     /translation="MIMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPAENPTGCYSLTFNIDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGKNRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PSTQISDFHVATHFNDDFSRAVLEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFG
                     GEIIDERGGYADRVTLRLNVENPALWSAEIPNLYRAVVELHTDDGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRFGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWSVALDGKPLASGEMPLDVAPQDKQLIE
                     LPELPQPESAGQLWLTVHVVQPNATAWSEAGHISAWQQWRLAENLSVTLPAAPHAIPQ
                     LTTSETDFCIELGNKRWQFNRQSGFLSQMWIGAEKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGSYHYQLVWCQK"
     gene            8269..>8533
                     /locus_tag="KLK57_003465"
     CDS             8269..>8533
                     /locus_tag="KLK57_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0893744.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFA"
BASE COUNT         1940 a         2390 c         2335 g         1868 t
ORIGIN      
        1 aactatggcc gcccatcgag ttgcccagca ggtggatttt ggcgatatcc agttgatcca
       61 ccacgctttt caggattcgt gcattaagat ccgatcgtga accactatta acgatcgaat
      121 cgcttttgcc ccaacccgga caatccagca ggatcacccg atagcccgcc tctaccagcg
      181 gatcgatatt gcggctgaag ttcgcccagc cagtagcacc cgggccggaa ccatgcagca
      241 ggacaacggt ttcgtcgcct tgtccgcagt cattaaaatg gatgcgcagc gttttacccg
      301 cttcttctac attcagaaaa cggctggtgg cggcttcggt ttgtggctga taactcatca
      361 tcttctcctg catattcagt tctctgttct ggcgcttaac gagccaaatc cggcaatcca
      421 ctccgggatt gggcggtaat aacgcccttc gctacgccag ttgccaaatg cagaaatagc
      481 ggcaaaagcg gcgacccagg ttttgatttc atgtgtcgac tttccggcaa tggcggaaag
      541 ctcttcgtta ctgacggcat ccagttcctg tatgcgtccc tgctccagca aagtcatgaa
      601 ctggttatcc caaatcgggt tgagcggatg cagagttctc tgatcctcaa caaacttctc
      661 agcggcgcta atcacccttt gctgacgcaa ttcgcgctca ctggcgggta aatctttccc
      721 gctccccaac agacggtcgc gcatatgggc atcggctttc gccagttcgg gcaccggcgg
      781 ctgatgggaa agcccaccgg aacccaggaa cagcacgcgt ttattgagag tgctggtgaa
      841 acgtccaatg gcttcaccca acatgcgggt acgctggaaa ccgggcagcg gcgtggcgac
      901 accgttgatg aacacaggca gaactggcac cttatccagc ccaccgagca ggaactccag
      961 cggctgggcg aacccgtggt ccacctgcat acagtaagaa acggcaagat cgatcccgct
     1021 cttcatgaca gcatgcgcac aggcctccgc cagctccaca ggcacgggca gctctcctgc
     1081 cgcactgccg aaatcaccaa ttgccgtcgc tccaacgcct aaacagaacg gtggcatcac
     1141 gtcatagaaa aagccgttgt agtgatctgg cgcaaacagc accaccagtt caggggagaa
     1201 agccgcaata cgctcgcggg cgctggcaat cacgccattg acctcatcga gtacctcttg
     1261 cgccgggtcg acatacccca ccaacggcga gtgggaaaga cagtgaagat aagcgtgcat
     1321 atcaggctac cttttcgaca gaaacgtcgg catcagggcg ggtcagcgtc atcaccgacg
     1381 ccagtttatt cagcgtattg cccagggttt gcggaatggc ggtggcggca acaaagcgat
     1441 ccgggcgcat caccaccagc gaagcattgt gttgcgcgaa ccagctacgc agacgacctt
     1501 gcgtatcgcc cacgcgtagc acgccgtcgt ggttatcctg tgcggtatga atttgcactt
     1561 ccggcaccac ctgaatgaag cgtgtgccca acgcgcgcca ctgctggatt tgctcgtcgc
     1621 tcatccccca cagtggattg catccccagc caattaccgc gaagttcgcg ccgatcgcgt
     1681 tatcgagcag cgtcacgtcg ccgttttcca gcgtgacttt cggctgaata aacatcttgc
     1741 cgaccggaga gtgcttcgct tcgccctcac gcaccagcgc accgccgtaa tattgcggca
     1801 tcggcttgaa gcgcatttcg aggaagtagc gttttactgg cggcagataa ttcaacagcc
     1861 aggaaacgcc gtcacgtaac gtaccctgcc agcgtttcgg cggagccagc acgttgcccg
     1921 ccgtcacgga caggtcaatc atcgctttgg cgtgatcgcg acgttcttgt tgataggtat
     1981 cgagcagcgc atcgcgggct ttcccctgga taaccaacgc cagtttccag gcgaggttaa
     2041 aggcgtcgcg cataccactg ttatagccct gcccctgcca caccggcatg atgtgcgcgg
     2101 catcgcccgc cagcagtacg cgatcaatac ggaaacgttg cgccagtcgc gcgttatggg
     2161 tgtagacacg ctggcgaatc aattcaacat tgtccggatt aggcagcact ttgcttaaca
     2221 gcttgcgcat attttgcggc tcacgcagct gctcttcggt ttctcccggc atcaccataa
     2281 attcaaagcg acgtaccgca tgaggcagcg cggcagaaac atacgggcgc accggatcgc
     2341 aacacaaata gatatgcggc gtacttaacg gatcgttggc gatatctacc acaatccact
     2401 gatttggcgc agttttacct tcaaacggca cattcagggt gcgacgaaca aaacttgccc
     2461 caccatcaca ggccaccagc cactgggctt tgatcgtttc ccgctgccct tctgccgttt
     2521 tcaggtgcaa ggtcacttcg tcatcttgct ggctgaaggc ctccagctcg cgggaaaaca
     2581 agcagcgcac attcggaaaa cgcgacaacc cttccagcat caccgcatcg acctgtggct
     2641 gaataaaggc gttacggcgc ggccagccaa attcatcggt cattggctga atatcagcaa
     2701 aacagcggcc ttttggggtg agaaaacgca tcgcgtgcca cggtgtagtg tgcggcagaa
     2761 cattctcgac caggccgacc gactgcatgg tgcgcagcgc ctcgtcatca ataccaatcg
     2821 cacgcgggta gtcgatcaac ttatcgagtt tctccaccac cagcacgtca atgcccatct
     2881 gaccgagata gttcgccatc atcagcccaa ccggaccggc accagcgatc gccacctgaa
     2941 cgctatggtt aacagcaggc tggatgtcag ggtgttgtat tgccatttct gcacaacgtt
     3001 gttttaacta tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc
     3061 gtgcgcggct taacccgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt
     3121 gccagcgtcg ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg
     3181 ctggagacgc tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt
     3241 ctgaccatca aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca
     3301 ctggcggccc cgctgctggg cgatctgttg cgcgaagtgg tatgaccaac agatgtgtcc
     3361 accctggatg ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc
     3421 tttcaccggg caatggtcgg gcgacgttta ccgctcctga aaaccgcctc gggcctgacc
     3481 tggctggcct tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agccgcccgc
     3541 cccggtgatg actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc
     3601 gcgcgcaaag agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc
     3661 tctatcgccg taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat
     3721 atggcgagcg caatgaccat tgaacaggca gcggaaaagc atcttccggc gctacaacgg
     3781 gtagcaaaac agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga
     3841 agcggcgtgc atttacgttg acaccatcga atggcgcaaa acctttcgtg gtatggcatg
     3901 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga
     3961 tgtcgcagag tatgccggtg tctcttatca gaccgtttct cgcgtggtga accaggccag
     4021 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
     4081 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
     4141 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
     4201 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
     4261 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
     4321 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
     4381 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
     4441 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
     4501 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
     4561 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
     4621 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
     4681 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
     4741 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     4801 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     4861 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     4921 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     4981 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     5041 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     5101 gtgaaattgt gagcggataa caatttcaca caggaaacag ctatgatcat gattacggat
     5161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     5221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     5281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
     5341 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
     5401 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat
     5461 cccattacgg tcaatccgcc ttttgttccc gcggagaatc cgacgggttg ttactcgctc
     5521 acatttaata ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggt
     5581 gttaactcgg cgtttcatct gtggtgcaac gggcgctggg tcggttacgg ccaggacagc
     5641 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggaaaaaa ccgcctcgcg
     5701 gtgatggtgc tgcgctggag tgacggtagt tatctggaag atcaggatat gtggcgaatg
     5761 agcggcattt tccgtgacgt ctcgttgctg cacaaaccga gcacacaaat cagcgatttc
     5821 catgttgcca ctcactttaa tgatgatttc agccgcgcgg tactggaggc agaagttcag
     5881 atgtacggcg agctgcgcga tgagctgcgg gtgacggttt ctttgtggca gggtgaaacg
     5941 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtggttat
     6001 gccgatcgcg tcacgctacg tctgaacgtc gaaaacccgg cgctgtggag cgccgaaatc
     6061 ccgaatctct atcgtgcggt ggttgaactg cacaccgacg acggcacgct gattgaagca
     6121 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
     6181 ggcaagccgt tgctgattcg cggcgttaac cgtcacgagc atcatcctct gcatggtcag
     6241 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
     6301 gccgtgcgtt gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgcttc
     6361 ggcttgtacg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
     6421 ctgaccgatg atccgcgctg gttacccgcg atgagcgaac gcgtaacgcg aatggtgcag
     6481 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgagtc aggccacggc
     6541 gctaatcacg acgcactcta tcgctggatc aaatctgtcg atccatcccg cccggtgcag
     6601 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
     6661 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
     6721 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
     6781 cttggcggct tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc
     6841 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaatgg caatccgtgg
     6901 tcggcttacg gcggtgattt tggcgataca ccgaacgatc gccagttctg catgaacggt
     6961 ctggtctttg ccgaccgcac gccgcatcca gcgctgacag aagcaaaaca ccagcagcag
     7021 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
     7081 catagcgata acgagcttct gcactggtcg gtggcgttgg atggcaagcc gctggcaagc
     7141 ggtgaaatgc ctctggatgt tgctccacaa gataaacagt tgattgaact gcctgaacta
     7201 ccgcagccgg agagcgccgg acaactctgg cttacggtac acgtagtgca accgaacgcg
     7261 accgcatggt cagaagccgg acacatcagc gcctggcagc agtggcgtct ggcggaaaac
     7321 ctcagcgtga cactccccgc cgcgccccac gccatcccgc aactgaccac cagcgaaacg
     7381 gatttttgca tcgagctggg taataaacgt tggcaattta accgccagtc aggctttctt
     7441 tcacagatgt ggattggcgc tgaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
     7501 cgtgcaccgc tggataacga cattggcgta agtgaagcga cccgcattga cccgaacgcc
     7561 tgggtcgaac gctggaaggc ggcgggccat taccaggccg aagcagcgtt gttgcagtgc
     7621 acggcagata cacttgccga cgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
     7681 ggaaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
     7741 attaccgttg atgttgaagt agcgagcgat acaccgcatc cggctcggat tggcctgacc
     7801 tgccagctgg cgcaggtagc agaacgggta aactggctcg gattagggcc gcaagaaaac
     7861 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
     7921 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
     7981 tatggcccac accagtggcg cggcgacttc cagttcaata tcagtcgcta cagccaacaa
     8041 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
     8101 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtttcagcg
     8161 gaattccagc ttagcgccgg tagctaccat taccagttgg tctggtgtca aaaataataa
     8221 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
     8281 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatgggg
     8341 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
     8401 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
     8461 ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
     8521 gtgatgtttg cgc
//
LOCUS       DAEEKQ010000162         8357 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.164, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000162 DAEEKQ010000000
VERSION     DAEEKQ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8357)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8357)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8357
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.164"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            85..1581
                     /gene="ravA"
                     /locus_tag="KLK57_003466"
     CDS             85..1581
                     /gene="ravA"
                     /locus_tag="KLK57_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HBE0893745.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRLQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIHQAQQC"
     gene            1575..3026
                     /gene="viaA"
                     /locus_tag="KLK57_003467"
     CDS             1575..3026
                     /gene="viaA"
                     /locus_tag="KLK57_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HBE0893746.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3031..4023)
                     /gene="asnA"
                     /locus_tag="KLK57_003468"
     CDS             complement(3031..4023)
                     /gene="asnA"
                     /locus_tag="KLK57_003468"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HBE0893747.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            4175..4633
                     /gene="asnC"
                     /locus_tag="KLK57_003469"
     CDS             4175..4633
                     /gene="asnC"
                     /locus_tag="KLK57_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HBE0893748.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLAEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4723..5166
                     /gene="mioC"
                     /locus_tag="KLK57_003470"
     CDS             4723..5166
                     /gene="mioC"
                     /locus_tag="KLK57_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HBE0893749.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5545..7434
                     /gene="mnmG"
                     /locus_tag="KLK57_003471"
     CDS             5545..7434
                     /gene="mnmG"
                     /locus_tag="KLK57_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HBE0893750.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLVGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7498..8121
                     /gene="rsmG"
                     /locus_tag="KLK57_003472"
     CDS             7498..8121
                     /gene="rsmG"
                     /locus_tag="KLK57_003472"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HBE0893751.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
BASE COUNT         2118 a         2117 c         2121 g         2001 t
ORIGIN      
        1 ctttgagact attttagcca ccagcaagaa acgtctatac tcgcaattta cgcagaactt
       61 ttgacgaaag gacgccactt cattatggct caccctcatt tattagcgga aagaatttcc
      121 cgcctgagca gttcgttgga aaaggggctt tatgaacgta gccacgccat ccgcttgtgt
      181 ttattagcgg cattaagtgg tgaaagtgtg ttcctccttg gcccgccagg tattgccaaa
      241 agtttgatcg cccggcgctt aaaattcgcc tttcagaatg cccgcgcgtt tgaatatctg
      301 atgacccgct tctccacgcc ggaagaagtt tttggtcccc tttctattca ggcgctaaaa
      361 gatgaagggc gctatgaacg tttaaccagc ggttacctgc cggaagcaga aattgtcttt
      421 ctggatgaga tctggaaagc gggcccggca attcttaata ccttgctcac cgccattaac
      481 gagcgccagt tccgcaacgg tgcacacgta gaaaaaatcc cgatgcgtct gctggtggcg
      541 gcctccaacg agctgccgga agcagacagc agtctggaag cgttatatga ccgcatgctg
      601 attcgtctgt ggttagataa agtgcaggat aaagcgaatt tccgctccat gctgaccagt
      661 caacaggatg aaaacgacaa tccggttcct gatgccttgc aggtcacaga tgaagaatat
      721 gaacgctggc agaaagagat tggtgaaatt acgctgcccg atcatgtatt tgagctgatt
      781 tttatgctgc gcctgcaact ggataaatta ccggatgcgc cttatgtctc ggatcgtcgc
      841 tggaaaaaag cgatccgatt attgcaggcc agcgcctttt ttagcggtcg cagtgctgtt
      901 gccccggtag atctcatttt gctgaaagat tgcctgtggt atgacgcgca aagcctgaat
      961 ttgatacaac aacaaattga tgtattgatg accggtcatg cctggcaaca gcaagggatg
     1021 ttgactcgcc ttggcgcgat tgtgcaacgt cacctgcaac tccagcagca acaaagcgat
     1081 aaaacagcct taacggtaat tcgtctgggc ggcattttca gccgtcgtca gcagtatcaa
     1141 cttcctgtta acgttactgc ttccactctg actctactgc tgcaaaaacc gttaaaactg
     1201 catgatatgg aagtggttca tatctccttt gagcgtagcg cgctggaaca gtggctgagc
     1261 aaaggtggtg aaattcgcgg taaactgaac ggtatcggct ttgcccagaa actgaatctg
     1321 gaagttgata gcgcccaaca tcttgtcgtt cgcgatgtaa gtttacaagg cagtacgctg
     1381 gcacttcccg gttcatcggc tgaaggtctg cctggtgaaa taaaacaaca actggaagag
     1441 cttgaaagcg actggcgcaa gcaacacgct ttattcagcg aacagcaaaa atgtctgttt
     1501 atccctggcg actggttagg tcgcattgaa gccagcctac aggatgtcgg tgcacagatt
     1561 caccaggcac aacaatgcta acgctggata cgcttaatgt gatgctggcc gtcagcgaag
     1621 agggattgat cgaagagatg atcatcgcac tgctggcctc accgcagctg gcggtcttct
     1681 ttgaaaaatt cccacgcctg aaggcggcaa tcactgatga tgttccccgc tggcgtgagg
     1741 cgctgcgcag tcggctgaaa gatgcccgag tcccgccgga actcaccgaa gaggtgatgt
     1801 gctatcagca aagccagctc ctctccacgc cgcagtttat tgtgcagcta ccacagatcc
     1861 tggacttact gcatcgtctg aattccccat gggcagaaca agcccggcag ttggttgatg
     1921 ctaacagcac aataacttca gcgttacaca cgctttttct ccagcgttgg cgtttaagtc
     1981 tgatcgtgca agcaacgacg ttaaatcaac agctattaga agaagaacgc gaacaactgt
     2041 tgagtgaagt tcaagaacgc atgacgctga gcggacaact tgaaccgatt ctcgcagata
     2101 acaatactgc agctggtcgt ctgtgggata tgagcgccgg tcagcttaaa cgtggcgact
     2161 atcagttgat tgtgaaatac ggtgaatttc ttaacgaaca gccggaactg aaacgcctgg
     2221 cagagcagct ggggcgttct cgggaagcca aatcaatacc gcgcaacgat gcgcagatgg
     2281 aaaccttccg caccatggtg cgcgaaccgg cgacggttcc tgagcaggtt gatggtctgc
     2341 aacaaagcga tgatatttta cgtcttctgc cgccagaact ggcgacacta gggataacgg
     2401 aactggagta tgagttttac cgtcggctgg tggaaaaaca gttgctcacc tatcgcctgc
     2461 acggtgagtc gtggcgtgaa aaagtgatcg aacgtccggt ggtacataaa gattacgatg
     2521 aacagccgcg cgggccgttt attgtctgtg tggatacttc cggctcaatg ggcggcttta
     2581 atgaacagtg tgcgaaagcg ttctgcctgg ccttgatgcg cattgctctc gctgaaaacc
     2641 ggcgctgcta tattatgcta ttttccaccg agatcgtccg ttatgagctt tcaggccccc
     2701 aaggcatcga acaagcaatc cgttttttaa gccagcagtt tcgtggcggc accgatcttg
     2761 ccagttgttt tcgcgccatt atggaacgct tgcaaagcag ggaatggttt gatgccgatg
     2821 cggtggtgat ttctgatttt atcgctcagc ggttgcctga cgacgtgacg agtaaagtga
     2881 aagagctgca gcgggtacat cagcatcgct ttcatgccgt ggcgatgtcg gcacacggca
     2941 aacccggcat catgcgcatt ttcgatcata tctggcgctt tgataccggg atgcgaagcc
     3001 gcctgctcag acgctggcgg cgataaatta ttacagcaga gaagggacgt tctcgcgaac
     3061 agcagctggc catacaccac actgaacctg gccgatatgc ggcagttgca gcagcagcat
     3121 ggtcaaacga gactggccga taccgccgcc gatggtctgc ggcatttcac cgcgcagcag
     3181 cgcctgatgc cattccagct gcagacgatc ttcatcaccg gtcagcgcca actgatgctt
     3241 caacgtgtcg gcatccacgc ggatccccat ggaagaaagc tcaaacgcat cttccagtac
     3301 agggttccac accagaatat cgccgttcag acccgcatga cccagctctg acggggtgct
     3361 ccagtcatca taatccggtg cgcgcacgtc gtggcgatga ccatcgctca gcttgccgcc
     3421 aatcccgaca aggaataccg cgccaagatc tttcgctatc gcccgctcac gccctttggc
     3481 atcgagatcc ggataacgag acagtaactc ctggctgtgt acgaagtgga tctgatccgg
     3541 caggaacggt gccaggccaa actcttcgct aaccgcagct tcggttgctt taattcccgc
     3601 ccagatcgcc tctaccgtgc ttttcagagt cgagaattga cgctcaccgt cgcccattac
     3661 gcgttcccag tcccactggt caacatagac cgagtgcaac ggagaaagac ggtcttcatc
     3721 ggggcgaagg gctttcatgt gcgtgtacag cccttcgccc gcgctgaagt cgtgttgccc
     3781 taaggtctga cgtttccact tcgccagtga atgaaccact tcgaactggg catcaggcag
     3841 agctttcact tttacctgca ccgctttttc acagcccgac aagttatcct gcgtgccatc
     3901 ccccacacgg ctaagaatcg gcgcctggac ttcgatcagc cccagacgtt cttccagttg
     3961 gcgagaaaag tgagatttca cgaagctaat ttggcgttgt ttggcaatgt aagcggtttt
     4021 cattttttat actcctgcgt cctgttgctt atgattaagc aacaaaaacc gacaaacatg
     4081 caataatcat tcaataaaaa gcctaacaca cttttgattc attaaaaaag aaggctaaaa
     4141 tagaatgaat catcaatctg cataagaaaa tcctatggaa aattatctga tcgacaatct
     4201 ggaccgtggc atcctggaag cattaatggg caatgcgcgc accgcttacg ccgaactggc
     4261 gaaacaattt ggcgtcagtc cggggacgat tcacgttcga gtagagaaaa tgaagcaggc
     4321 ggggatcatt accggggcgc gtattgatgt cagcccgaag cagctcggtt atgacgtagg
     4381 ctgctttatc ggcattatat taaagagcgc caaagactac ccttccgcgc tggcaaagct
     4441 ggaaagcctt gctgaagtca ctgaagccta ttacacaaca ggccactaca gcatctttat
     4501 aaaagtaatg tgccgttcga tcgacgctct ccagcatgta cttatcaaca agatccaaac
     4561 aattgatgaa attcagtcca ccgagacatt gatcgtcctg caaaacccga tcatgcgtac
     4621 catcaagcca taatcggctt ttttaatccc atacttttcc acaggtagat cccaacgcgt
     4681 tcacagcgta caatacgcca ctcttaataa aggtggcggt ttatggcaga tatcactctt
     4741 atcagcggca gcaccctcgg cggtgccgaa tatgtagcag aacacctggc tgaaaagctg
     4801 gaagaggcgg gttttaccac cgaaacgctg cacggtccgc tgttggaaga tttatctgcc
     4861 tcggggatct ggctgattat cagctccacc cacggtgccg gagatattcc ggacaacctt
     4921 tctcctttct atgaagcatt gcaggaacag aagcccgatc tttctgcggt ccgctttggc
     4981 gcaatcggta ttggtagtcg tgaatatgac accttttgtg gggctatcga taaactcgag
     5041 gcagaactca aaaattccgg tgcaaaacag acaggcgaaa cactgaagat caacattctt
     5101 gatcacgaca ttccggaaga tccggcagaa gaatggctgg gatcgtggat taatttactc
     5161 aaataagtat acagatcgtg cgatctactg tggataactc tgtcaggaag cttggatcaa
     5221 ccggtagtta tccaaggaac aacggttgtt cagtttttga gttgtgtata acccctcatt
     5281 ctgatcccag cttatacggt ccaggatcac cgatcattca cagttaatga tcctttccag
     5341 gttgttgatc ttaaaagccg gatccttgtt atccacaggg tagtgcgatc ctaataagag
     5401 atcacaatag aacagatctc taaataaata gatcttcttt ttaataccca ggatcccagg
     5461 tctttctcaa gccgacaaag ttgagtagaa tccacggccc gggcttcaat ccattttcat
     5521 accgcgttat gcgaggcaat caccatgttt tatccggatc cttttgacgt catcatcatt
     5581 ggcgggggtc atgcaggcac cgaggccgcg atggccgcgg cgcgtatggg tcaacagact
     5641 ctgcttttga cacacaatat cgacactctg gggcagatga gctgcaaccc ggcgatcggc
     5701 ggtattggga agggacatct ggtaaaagaa gtggatgcac tcggcggtct gatggcgaaa
     5761 gcgatcgatc aagcgggtat ccagtttagg atactaaacg caagcaaagg accggcggtt
     5821 cgcgctaccc gagctcaggc ggatcgtgtg ctctaccgtc aggcggtacg tacggcgctg
     5881 gagaaccaac cgaacctgat gatcttccag caggcggttg aagatcttat tgtcgaaaac
     5941 gatcgcgtgg tcggtgctgt tacccaaatg ggactgaagt tccgtgccaa agccgtcgtg
     6001 ctcaccgttg ggacgttcct cgacggtaaa attcatatcg gtctggataa ttacagcggt
     6061 ggtcgtgctg gtgatccgcc gtccattccg ctttctcgcc gtttgcgtga actgccgctg
     6121 cgcgttggtc gtctgaaaac cgggacacca ccgcgtattg atgctcgaac tatcgacttt
     6181 agtgtgctgg cgcaacagca tggcgataac ccaatgccgg tattctcgtt tatgggcaat
     6241 gcgtcccagc atccccagca ggtgccgtgt tatatcactc ataccaacga gaaaacccat
     6301 gatgtgatcc gcagtaacct cgatcgtagc ccaatgtacg caggggtgat cgaaggtgtc
     6361 ggcccacgct actgcccatc gatcgaagac aaagtcatgc gcttcgccga cagaaatcag
     6421 catcagatct tccttgaacc ggaagggctg acctctaacg aaatttatcc gaacggtatc
     6481 tccaccagcc tgccgttcga tgtgcagatg caaatcgtcc gctccatgca ggggatggaa
     6541 aacgcgaaga tcgtgcgtcc gggttatgcc attgagtatg acttcttcga tccacgcgac
     6601 ctgaaaccga cgctggagag caagtttatc caggggctgt tctttgctgg tcagattaac
     6661 ggcactaccg gttacgaaga agccgctgcg caaggtttgc tggtcggtct taacgctgcc
     6721 cgtctgtctg ctgacaaaga aggttgggct ccggcgcgtt ctcaggcgta tctcggcgta
     6781 ctggttgatg acctgtgcac tttaggaacc aaagaaccgt atcgtatgtt tacttcgcgc
     6841 gcagaatatc gtctgatgct gcgcgaagat aatgcggatc tgcgtttgac tgaaatcggt
     6901 cgtgaactgg gcctggtgga tgacgaacgt tgggcgcgct ttaacgagaa acttgagaat
     6961 atcgagcgtg agcgtcagcg tctgaaatcg acctgggtaa ccccgtcggc ggaagctgca
     7021 gccgaagtga atgctcacct gactgcgccg ctttcccgtg aagccagtgg tgaagatctg
     7081 ctgcgtcgtc cggaaatgac ttatgaaaaa ttaaccacgc tgacgccgtt tgcccctgcg
     7141 ttgacagacg aacaggcggc ggaacaggtt gagattcagg ttaaatacga aggttatatc
     7201 gcgcgccagc aagatgagat cgaaaagcag ctgcgtaacg agaacaccct gctacccgcg
     7261 acactggatt accgccaggt atccggtctt tctaacgaag tgatcgccaa acttaacgat
     7321 cacaaaccgg cctctatcgg ccaggcttcg cgtatttctg gcgtcacgcc tgcggctatc
     7381 tccattctgc tggtgtggct gaaaaaacag ggtatgctgc gtcgtagcgc ataacgcatt
     7441 aaaaatgcct ggtaagcacc cgcttaccag gcaacgcatc aagaacaggt aatcaccgtg
     7501 ctcaacaaac tctccttact gctgaaagac gcaggtattt cgcttaccga tcaccagaaa
     7561 aaccagctta ttgcctacgt gaatatgctg cataaatgga acaaagcgta caacctgact
     7621 tcggtccgcg atcctaatga gatgctggta cgccatattc tcgatagcat tgtggtggca
     7681 ccgtatctgc aaggtgaacg gtttatcgat gtcggcaccg gaccaggact gccaggcatt
     7741 ccactctcta tcgtgcgtcc tgaagcccat ttcactctgt tggatagcct tggtaaacgc
     7801 gtgcgtttcc ttcgtcaggt gcaacatgag cttaaactgg agaatattga gccagtacag
     7861 agcagggtag aagagtttcc ttcagagccg ccatttgatg gcgtaattag ccgcgctttt
     7921 gcctctctga acgatatggt gagctggtgc caccatcttc ctggtgagca aggccgtttc
     7981 tacgcgctga aagggcaaat gccggaagat gaaatcgctt tgttgcccga agaatatcag
     8041 gtcgaatcag tggttaaact tcaggttcca gctctggatg gcgaacgtca tctggtggtg
     8101 attaaagcaa ataaaattta atttttatca aaaaaatcat aaaaaattga ccggttagac
     8161 tgttaacaac aaccaagttt tctactgata taactggtta catttaacgc cacgttcact
     8221 cttttgtatc aacaagataa cgtggctttt tttggtaagc agaaaataag tcattagtga
     8281 aaatatcagt ctgctaaaaa tcggcgctaa gaaccatcat tggctgttaa aacattatta
     8341 aaaatgtcaa tgggtgg
//
LOCUS       DAEEKQ010000163         8335 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.165, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000163 DAEEKQ010000000
VERSION     DAEEKQ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8335
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.165"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            488..1363
                     /locus_tag="KLK57_003473"
     CDS             488..1363
                     /locus_tag="KLK57_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ChaN family lipoprotein"
                     /protein_id="HBE0893752.1"
                     /translation="MRKFILISMITLASVSLGACRQNVTIKQDAPGQKAFLTDGQIYD
                     LHSGKIISSSELLADLATARHLIIGEKHDNAEHHQIELWLIQNLLIQRPQGSVLLEML
                     TSEQQPRVNQVKCWLKDNPVVRDSRVQELLNWQKGWSWEMYGDIVMQLLRGPYPLLNA
                     NIGREQMLALYKKNEFPKGKKSTAPVVQEALRETIISMHEGNLESQQLTSMLSIQQQR
                     DRYMARQLLSAPVPSLLIAGGYHASKSMGVPLHMEDLATGTHPVVLMLAEKGMNITVD
                     HADYVWFVAPDTTKR"
     gene            1371..2147
                     /locus_tag="KLK57_003474"
     CDS             1371..2147
                     /locus_tag="KLK57_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000981087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HBE0893753.1"
                     /translation="MMNILHFSQSVKWSSWFICSLLLHGLIFLAFIWRFSEVQPAMSP
                     APAIMLQWAEIIEAPSSPLSLPVGIAQQESAVTEEKQQTEDRQQRPVTEDSDATIEIT
                     RKKKSSDGEKKKTRPPRKIKAQTSDSNPTAVSSNAAPQALVESSRIAAPFNSDSTKRD
                     NSEASWESRVKGHLNRYKRYPGDARKRARTGTAVVTFTVNTEGTIVSSFLEISSGTLS
                     LDREAIAVLERAQPLPKPPPEILEGGLFKVKMPITFKLKE"
     gene            2316..4577
                     /locus_tag="KLK57_003475"
     CDS             2316..4577
                     /locus_tag="KLK57_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBE0893754.1"
                     /translation="MNVIKLAIGSGILLLSCGAYSQSISEKTNSDKKGAAEFSPLSVS
                     VGKTTSEQEALEKTGATSSRTTDKNLQSLDATVRSMPGTYTQIDPGQGAISVNIRGMS
                     GFGRVNTMVDGITQSFYGTSTSGTTTHGSTNNMAGVLIDPNLLVAVDVTRGDSSGSEG
                     INALAGSANMRTIGVDDVIFNGNTYGLRSRFSVGSNGLGRSGMIALGGKSDAFTDTGS
                     IGVMAAVSGSSVYSNFSNGSGINSKEFGYDKYMKQNPKSQLYKMDIRPDEFNSFELSA
                     RTYENKFTRRDITSDDYYIKYHYTPFSELIDFNVTASTSRGNQKYRDGSLYTFYKTSA
                     QNRSDALDINNTSRFTVADNDLEFMLGSKLMRTRYDRTIHSAAGDPKANQESIENNPF
                     APSGQQDISALYTGLKVTRGIWEADFNLNYTRNRITGYKPACDSRVICVPQGSYDIDD
                     KEGGFNPSVQLSAQVTPWLQPFIGYSKSMRAPNIQEMFFSNSGGASMNPFLKPERAET
                     WQAGFNIDTRDLLVEQDALRFKALAYRSRIQNYIYSESYLVCSGGRKCSMPEVIGNDW
                     EGISDEYSDNMYIYVNSASDVIAKGFELEMDYDAGFAFGRLSFSQQQTDQPTSIASTY
                     FGAGDMTELPRKYMTLDTGVRFFDNALTLGTIIKYTGKARRLSPDFEQDEHTGAIIKQ
                     DLPQIPTIIDLYGTYEYNRNLTLKLSVQNLMNRDYSEALNKLNMMPGLGDETHPANSA
                     RGRTWIFGGDIRF"
     gene            4646..5821
                     /gene="senB"
                     /locus_tag="KLK57_003476"
     CDS             4646..5821
                     /gene="senB"
                     /locus_tag="KLK57_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterotoxin production-related protein TieB"
                     /protein_id="HBE0893755.1"
                     /translation="MNIFTLSKAPLYLLISLFLPTMAMAIDPPERELSRFALKTNYLQ
                     SPDEGVYELAFDNASKKVFAAVTDRVNREANKGYLYSFNSDSLKVENKYTMPYRAFSL
                     AINQDKHQLYIGHTQSASLRISMFDTPTGKLVRTSDRLSFKAANAADSRFEHFRHMVY
                     SQDSDTLFVSYSNMLKTAEGMKPLHKLLMLDGTTLALKGEVKDAYKGTAYGLTMDEKT
                     QKIYVGGRDYINEIDAKNQTLLRTIPLKDPRPQITSVQNLAVDSASDRAFVVVFDHDD
                     RSGTKDGLYIFDLRDGKQLGYVHTGAGANAVKYNPKYNELYVTNFTSGTISVVDATKY
                     SITREFNMPVYPNQMVLSDDMDTLYIGIKEGFNRDWDPDVFVEGAKERILSIDLKKS"
     gene            complement(6347..6808)
                     /locus_tag="KLK57_003477"
                     /pseudo
     CDS             complement(6347..6808)
                     /locus_tag="KLK57_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            complement(6846..8335)
                     /locus_tag="KLK57_003478"
                     /pseudo
     CDS             complement(6846..8335)
                     /locus_tag="KLK57_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT         2201 a         2058 c         1995 g         2081 t
ORIGIN      
        1 ccgttccgct ttcaccgcct gaatgtcggg ctattggcaa gagcattgcg aaatacacgc
       61 acaggaactt cacgccggaa actttcgcac agtatgtggc tgatacgcac acattaacgt
      121 acgttttcgt tccattggcc ctcacccccc gataacgacc caggtagcgc gccgtattcc
      181 ccccgccggc cgttttcttc gacatgtaca tcaggcgcgg aactcatcgg ggcgcgggaa
      241 tgaaatccca ttcagactcc ggatagattc aagcaagccc acttatcgtc aaaatcggtg
      301 ttgcaaaaac gggagtgacc atagatccgt tttccaagcg gaccccataa tatcggctgg
      361 taaacatcac tcgctagtgc attctttttg ttggtaatga taatcattta cattcattct
      421 ttgatgtttt atcataatta atgttttgtc aataaatata tggaaacata ttgtttcaga
      481 tgggaatatg agaaagttca tccttatttc tatgataacg ttagctagtg ttagtctggg
      541 agcctgcaga cagaatgtca ccataaagca ggatgcgcca gggcagaaag ctttcctgac
      601 tgacgggcag atttacgatt tgcacagtgg caaaattatt tctagcagcg agcttttggc
      661 ggatcttgct accgctcgac atcttattat tggtgaaaaa cacgataatg ccgagcatca
      721 tcagattgag ctttggctta ttcaaaattt gctcatccag cgaccacagg ggagtgtgtt
      781 gcttgagatg ttgacgtcag agcaacagcc ccgagttaac caagttaaat gttggctgaa
      841 agataatcct gtggttcgag atagccgtgt tcaggaacta cttaactggc aaaaagggtg
      901 gtcctgggag atgtatggcg acatcgtcat gcagctatta cgtgggcctt acccgctgct
      961 caatgccaac attggccggg agcagatgtt ggcactgtac aaaaaaaacg aatttcctaa
     1021 aggaaaaaaa tccactgcac ctgtggtaca agaagctctg cgtgaaacca tcatatctat
     1081 gcatgaaggc aaccttgaaa gccagcaact gacgtcaatg ctctctattc agcagcagcg
     1141 ggatcgctac atggcccgcc agcttctgag tgccccggta ccgtcgttgt tgattgcagg
     1201 tggttatcac gcgtcaaaaa gcatgggcgt accgttacac atggaagatc ttgccaccgg
     1261 cacccatcca gtggtgttga tgcttgcaga aaaaggaatg aacatcacag tagatcatgc
     1321 tgactacgtg tggtttgtag cgccagatac aacaaaaagg taaaccatgg atgatgaata
     1381 ttctccactt ctcacagtcg gtaaaatgga gcagctggtt tatctgttct ctactgcttc
     1441 atggactgat tttccttgcg tttatctggc gtttttccga agttcagccc gctatgtctc
     1501 ctgcaccggc gatcatgctg caatgggcag aaattataga ggctccttct tctccacttt
     1561 cgcttccggt gggaatcgct caacaggagt ccgcagtgac agaagaaaag caacaaactg
     1621 aggacagaca acaacgtcct gtcactgaag acagtgatgc aaccattgaa ataactagga
     1681 agaaaaagtc atccgatgga gaaaagaaaa agacacgtcc acctcgtaag ataaaggctc
     1741 aaaccagcga cagcaacccc acagccgttt ccagtaatgc tgcacctcag gctctggtcg
     1801 aatcgtcccg tattgctgcg ccttttaaca gtgactcaac aaagcgagat aattctgagg
     1861 catcttggga aagcagagta aaaggccacc taaaccgtta taaacgttac cctggcgatg
     1921 ccagaaaacg tgcacgaacc ggcacagccg tcgtgacttt taccgtaaac acggaaggca
     1981 ctattgtcag tagtttcttg gaaatctctt caggaaccct ttctcttgac cgcgaagcta
     2041 ttgctgtttt agaacgcgct caaccacttc ccaaaccacc gcctgaaata ttggagggag
     2101 gattatttaa agtaaaaatg ccgattacct ttaagttgaa ggagtaaagg gataattggc
     2161 cgttcaagtt catttaactg aaatttaatt ttcattttta tgctgaacat tttatcaaac
     2221 ttttccactg tcgaaaaaat caaaaagaca gtagaacata attaataaac aattagttat
     2281 acttatcttt attaatatta tacccaagga aatttatgaa cgttataaaa ctggctatcg
     2341 gctcaggcat attattgctc agctgcggtg cttactcaca atccatcagt gaaaaaacta
     2401 attccgacaa aaaaggagcg gcagaattca gtccgctcag cgtttctgtc gggaagacga
     2461 ccagtgagca ggaagctctc gagaaaacag gcgcgaccag ttcccggaca acggacaaaa
     2521 acctgcaatc acttgacgca acagtgcgta gtatgcctgg tacttatact caaatagatc
     2581 ctggtcaggg agcaatcagt gtgaatattc gaggcatgag cggatttggt cgtgtaaaca
     2641 ctatggtcga tggtattacc cagagttttt acggaacctc tacctccgga acaacgacgc
     2701 atggttcaac taacaatatg gctggcgtac ttatagatcc taacttactg gtagcagttg
     2761 atgttacacg cggtgacagc agtggctctg aagggatcaa cgcccttgcc ggtagtgcaa
     2821 atatgcgtac tattggcgtt gacgatgtaa tatttaacgg taatacatat ggccttcgtt
     2881 cacgtttctc tgtcggtagt aatgggctgg gacgcagcgg aatgatcgcc cttggtggaa
     2941 aaagcgacgc ttttacggat acgggaagca ttggcgttat ggctgctgtg agcggcagtt
     3001 ctgtgtactc taatttctca aatggttctg gaattaacag caaagagttt ggttatgata
     3061 aatatatgaa gcagaacccc aaatcccaac tgtataaaat ggatatcaga ccagacgaat
     3121 ttaacagctt cgaactttcc gctcgaacct atgaaaataa atttacacgt cgtgatataa
     3181 ccagtgacga ctattacatt aaatatcatt acaccccttt ttctgaatta attgacttta
     3241 acgtaacggc cagtaccagt cgcggtaatc aaaagtatcg tgatggctcg ctgtatactt
     3301 tctacaaaac ctcagcgcaa aatcgttctg acgcgctgga tatcaacaat accagccggt
     3361 tcactgtcgc ggacaatgac ctggagttta tgctgggcag caaactgatg cgtacccgct
     3421 atgaccggac cattcactca gcggcgggcg acccgaaagc gaatcaggaa tcgatcgaga
     3481 acaatccgtt cgcaccctcc ggccagcagg atatttcagc gctgtatacc gggctgaagg
     3541 ttacgcgcgg catctgggag gcagatttca atctcaacta cacacgtaac aggatcacag
     3601 ggtacaagcc cgcctgcgat tcacgcgtta tctgcgtgcc acagggtagc tacgatattg
     3661 acgataaaga gggtggcttc aacccttcag ttcagctttc tgctcaggta acaccatggc
     3721 ttcagccgtt cattggctac agcaaatcca tgcgcgcccc gaacatccag gagatgttct
     3781 tctctaattc aggaggcgca tccatgaacc cattcctgaa gcctgaacgt gcagaaacct
     3841 ggcaggcggg ttttaacatt gataccagag atttactggt cgaacaggat gccctgcgct
     3901 ttaaggctct ggcgtaccgc agcaggatcc agaactacat ctacagcgag tcttatctgg
     3961 tttgttctgg aggtcgtaaa tgcagtatgc ctgaggtgat tggcaatgac tgggagggca
     4021 ttagcgatga atacagcgac aatatgtaca tctacgttaa ctcggcaagc gacgttatcg
     4081 caaagggctt cgaactggag atggattatg atgcaggttt tgcttttggc cgactctctt
     4141 tcagccagca gcaaacagac cagccaacct ccatcgccag cacctacttt ggcgcagggg
     4201 atatgaccga actgcccaga aaatacatga cgctggatac tggtgttcgc ttcttcgata
     4261 acgcgttgac cctgggcact atcataaaat acacaggcaa ggctcgtcgc ctgtcgcctg
     4321 attttgagca ggacgaacat accggcgcaa taatcaaaca ggatttgccg cagatcccaa
     4381 cgattatcga tctctatggt acttacgagt acaaccgcaa cctaacactg aaactttcgg
     4441 tacaaaacct gatgaacaga gattattcgg aggcgctgaa taagctcaac atgatgccag
     4501 gtcttggtga cgagacccac ccagccaatt ccgcgcgtgg cagaacatgg atatttggcg
     4561 gggacattcg tttctgatga caatttccgc taggttcaga caatacgttt tctctcttat
     4621 gtcaatttta ttgcaggaac gaaaaatgaa tattttcact ttatccaaag caccgctata
     4681 cctgttaatt tcactatttt tacccacgat ggccatggct atcgatccac ctgaacgcga
     4741 actttcgcga tttgccctga aaacgaatta ccttcagtcc cctgatgaag gcgtctatga
     4801 actggcgttt gataatgcca gtaaaaaggt gtttgcagca gtcaccgatc gtgtaaatcg
     4861 tgaagccaat aaaggctatc tgtattcgtt taattcagat tcgctgaaag tcgaaaataa
     4921 atacacgatg ccataccggg cattttcgct ggcgataaat caggataaac atcagctcta
     4981 tatcggacac acccagtcag cgtccctgcg tatcagtatg tttgacaccc caaccggcaa
     5041 actggtaaga accagcgaca ggttaagttt taaagcggca aacgctgcag attcgcgttt
     5101 tgagcatttt cgccatatgg tttacagcca ggattccgat accctgtttg tgagttatag
     5161 caatatgctg aaaacggccg agggcatgaa gcctctgcat aagctgttaa tgctcgacgg
     5221 gacgacgctt gccttaaaag gcgaggttaa ggatgcttac aaaggtacag cgtatggtct
     5281 gacgatggat gaaaaaacac agaaaatcta cgttggcgga agagattaca tcaacgaaat
     5341 tgatgcgaaa aatcagacgc tgctgcgtac catcccgttg aaagatccga gaccacaaat
     5401 cacaagtgtg cagaatctgg cggtggactc cgcttctgac cgtgcctttg tggtggtatt
     5461 cgaccatgac gatcgttccg gtacaaaaga tggactctat atttttgact tacgcgacgg
     5521 taaacagctt ggctatgtgc acacaggagc cggagctaac gcggtgaaat acaatccgaa
     5581 atataacgaa ctgtatgtca ccaacttcac tagcggcacc atcagcgtag tggatgccac
     5641 caaatacagc atcacccgtg aatttaacat gccggtctac ccaaaccaga tggtgttgtc
     5701 ggacgatatg gatacccttt acattggcat caaagaaggc tttaaccgcg attgggatcc
     5761 tgatgtgttt gtggaaggag ctaaagaacg tattctgagc attgatttga aaaagtcgtg
     5821 atcgggggga aaaaacatgg gataaaaact ccgttattcc actaaactgg ccggactagg
     5881 aaaagcaaac atctctgtcc tgccggtacg cctgttacgc cgctccatga ggctgaggaa
     5941 tctccagaaa acgagacagg cacagcattg tacgatcaac tgatttgtgc caataattaa
     6001 atgaaatcac agctatgcct gttcatgggg ttctaggccg gaaccccaga tttttccgat
     6061 tgtttgtttt ctaactgatt attcagtagt tttagagggc tgacgctcag tggaacgaaa
     6121 actcacgtta agaggttttg actatgataa aagtcaaagt aggggtgagt ttggatatgg
     6181 aaattattgt actataagga gagcatgaaa acccctgggg cagtcactga atgagggacc
     6241 agcagcggta aaataaccag cataatttct gcatggtcga tttttatacc gtgacaggct
     6301 gttttttata cttggggtca gtttggatat cgaaaattat tgtacgttaa ggttgttttt
     6361 tgaccttgtc gatcttaact gatctcaaac gacgaaactt acctgaagtc ggtatccggc
     6421 tgtttttcca gatataatcg cctgtcagat tgatatgttc ccaacctaat ggtgataaat
     6481 gcgataatag ttgagcattt attttcatgc ccttacgctt cagagcatca accgctcttt
     6541 ccatgtaaac tgtgttccat aaggaaatag ccgccgtcaa taaagtcaat ccactggcgc
     6601 gataactttg attttccggt ttccgatctc taatctcccc caatcgatgc atgaatacag
     6661 cacgggccag agcattacgg gcttctcctt tgttcagccc ggcctggaca cgtcgccgca
     6721 atgccggatc ccgaaaccag tccagcataa acagtgttcg ttctatacgg ccaatctctc
     6781 tcagagcttt agccaaccca ttttgtttgt aagcgtgcag caagaaccgt attgacggga
     6841 atgtgttatt cagtcggcag tgctacgcgc caggggagca gttcgccgac ccggtttatc
     6901 ggccagtcgg ctatgacgtc aaggacatag cggaggtagc tttctggctc cactccgttc
     6961 agtttgcacg tcccgatcag gctgtacagc agcgctcccc gctctcctcc atgatccgaa
     7021 ccgaagaaca ggtagttttt gcggcccaga ctgaccatcc gcaacgcatt ttcagcgatg
     7081 ttattgtccg cctcagccca gccatcatct gcatagtacg tcagcgccgg ccactggttc
     7141 agggcgtatg cgaacgcttt cgccagttct gagtgtcgcg acagggtttt catcttttca
     7201 cgcagccagc tttccaggga tttcagcagc ggtttcgttt tcaactgacg ttcggcaagg
     7261 cgctgttccg ccgtcattcc ccttatctct gcctcgatgg cgtacagttc gccgatccgt
     7321 ttcagcgctt cctccgtcag ggctgacggg gtgcgaacgt gcacatcgtg gattttacgg
     7381 cgggcgtgag cccaacaggc ggcttccgtt atccggccat cccggtacag ctcgttgaac
     7441 ccggcgtatg catccgcctg cagtacacca ctgaaccccg caagatgggt ctgcggatgg
     7501 atgccttttc tgtccgggct gtaagcgaac cacaccgccg gcgccagcgt tgacccggcg
     7561 ttacggtcgt cacgaacata cgccacaacc gcccggtctt cgttttctta ttgcctggca
     7621 acagcaccgg gacaggcgtg tcatcaacat ggagcttacc gtcagtcagc acatagccat
     7681 gaagcgcctc ttccatcgga gacagcagcc ggcagcatgc atccacccag cccgacagca
     7741 gtgaacggct cagctccaca ccttgccggc cgtatatttc tgactggcga tacagcgggg
     7801 tgtgctctgc atacttcgag gtcagcacgc gggccagcag ccccggtccg gcgatacccc
     7861 gctcgatggg ccgcgaaggt gcaggtgcct gcacgatggc atcgcactga gtacaggcat
     7921 gtttttcccg taccgtccgg ataacccgga aggcgctacg catcaactcc agctgttcgg
     7981 cggtatcctc gcccagatag ctcagtgaac cgccgcagtt cgggcagcac ggcgccgcag
     8041 gcaacagtcg cttttcgtca cggggtagtg attcagggaa cggcttacgg gtgcgggtct
     8101 gacgcaacgg acgctgtact gccgggtcat acaccctacc agtcagcgta tcgctctctt
     8161 tctgaagccg gttcagatcg gcttccattt gtgcgatacg gcgggagact ttttcggaac
     8221 gactgccgaa gttcatccgg cggagtttat ccagctgcgc ctgcagatgg tctatttcgc
     8281 gctcccggtt gctaagcttt tcctgcaggg cgtggatcag cgcttcctgt tcggc
//
LOCUS       DAEEKQ010000164         8326 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.166, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000164 DAEEKQ010000000
VERSION     DAEEKQ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8326
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.166"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            267..1925
                     /locus_tag="KLK57_003479"
     CDS             267..1925
                     /locus_tag="KLK57_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HBE0893756.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNMEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIVTTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(1922..2878)
                     /locus_tag="KLK57_003480"
     CDS             complement(1922..2878)
                     /locus_tag="KLK57_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HBE0893757.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3029..4144
                     /gene="macA"
                     /locus_tag="KLK57_003481"
     CDS             3029..4144
                     /gene="macA"
                     /locus_tag="KLK57_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HBE0893758.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4141..6087
                     /gene="macB"
                     /locus_tag="KLK57_003482"
     CDS             4141..6087
                     /gene="macB"
                     /locus_tag="KLK57_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HBE0893759.1"
                     /translation="MTPLLELKDIRRSYPAGNEQVEVLKGISLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="KLK57_003483"
     CDS             complement(6160..6384)
                     /gene="cspD"
                     /locus_tag="KLK57_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HBE0893760.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6789..8027
                     /locus_tag="KLK57_003484"
     CDS             6789..8027
                     /locus_tag="KLK57_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE0893761.1"
                     /translation="MSSAIHRLSDTLLRKLSGSPTTKNAFFNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRQVSPERLRLGNYPDLSLKAAREKAAQCRAWLAEGKNPRYELNRAVQD
                     ALAPVTVKEALTYWLESYAKEKRTDYESLKSRINKHIISQIGALPLEKCELRHWLACF
                     DQMAKRSPVSAGFLLQVCKQALKYCRKRRYAISNVLDDMVVGDVGKKAEISERVLTNK
                     ELGELLRALDEKIYPPYYSALIRLLIVFGCRATELRRSEVQEWDFKEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTKLVEQNRHTGLLLGELKGQSSVSEYGRTAHRRINQAPWTL
                     HDIRHTFTTMLNDLGVDPHVVEQLTAHQLPGVQRVYNHSRYLDAKRDALNLWVERLEL
                     LQNNDEKIVVMTPRIYSQNS"
BASE COUNT         2057 a         1960 c         2233 g         2076 t
ORIGIN      
        1 ggttgcccca tatagtcatt tgtctgattg acagtgtagt gcacgcaaaa gatttaatcc
       61 tttaggcgta ataaaaaata atatatcatg ctaattattt gattttgttg tttttgcaga
      121 cttatcagca agagggagta taacgcgatt attcgctcat ttttcagaca tttgccatgc
      181 ttaaatgtga tgtcatcacg tattagcaag gcctttcccg ttatactgcc agcgtaaagg
      241 ataagtcaca tatttctgga ggggatatga ttcttgagcg cgttgaaatt gtgggttttc
      301 gcggtatcaa ccgtttgtcg ttgatgctgg aacaaaacaa cgtcctgatt ggggagaacg
      361 cgtggggtaa atccagcttg ctggacgcct taaccctgct gctatcgcca gaatcagatc
      421 tctaccattt tgagcgcgac gatttctggt tcccgccggg agatatcaac gggcgagaac
      481 atcatctgca tattattttg accttccgcg aatcgctgcc aggccgacat cgggttcgcc
      541 gttatcggcc gctggaagcg tgctggacgc catgcaccga tggctatcac cgtatttttt
      601 atcgtctgga aggggagagt gcggaagacg gcagcgtgat gacactgcgc agttttctcg
      661 ataaagacgg acatccgatt gatgtcgagg atattaacga tcaggcacgc catctggtgc
      721 gtttaatgcc ggtgctgcgc ttgcgtgatg cccgttttat gcgccgtatt cgtaacggca
      781 cggtgccaaa tgtccctaat atggaagtca ccgcgcgcca gctcgatttc ctcgcccgtg
      841 agttatcctc acatccgcaa aatctctctg atgggcagat tcgtcaggga ctttccgcaa
      901 tggtacagct gcttgagcat tatttctctg agcagggggc cggacaggcg cgatatcgtt
      961 taatgcggcg gcgagccagc aatgagcaac gaagctggcg ctatctggat atcatcaacc
     1021 ggatgattga ccgacctggt gggcgctcgt atcgggttat tttgctcggc ctgtttgcta
     1081 ctttgttgca ggcaaaaggc acattgcgac tggataaaga cgcccgtcca ttgttgctga
     1141 tcgaagatcc agaaacccgt ttacacccca ttatgctttc agttgcctgg catctgttga
     1201 atcttctgcc attgcagcgc attgtcacca ccaactcggg tgagttgctt tcgttaacgc
     1261 cggtagagca tgtttgccga ctggtacgtg agtcctcgcg cgttgccgcc tggcgtctgg
     1321 ggccgagtgg cttgagtacc gaagatagcc gacgcatatc ctttcacatt cgttttaacc
     1381 gtccgtcatc gctgtttgca cgctgctggt tgctggtgga aggggaaacg gaaacctggg
     1441 ttatcaatga actggcgcgt cagtgcggac atcattttga tgccgaaggg atcaaggtca
     1501 ttgagtttgc ccagtccggg ctaaagccac tggttaaatt tgcccgccga atggggattg
     1561 aatggcatgt actggtcgat ggcgatgaag cagggaagaa atatgccgct acggtacgca
     1621 gcctgttgaa taacgatcgg gaagccgaac gagaacattt aacggcgtta ccggcgctgg
     1681 atatggaaca ttttatgtat cgccagggat tttccgatgt gttccaccgc gtggcgcaaa
     1741 tcccggaaaa tgtaccgatg aatctacgca aaattatctc gaaagcgatc catcgctctt
     1801 ccaaacccga tcttgccatt gaagtggcaa tggaggcagg acgtcgtggt gtggactccg
     1861 taccgacgct gctgaaaaaa atgttctcac gcgtgctgtg gctggcgcgc ggtcgcgcgg
     1921 attaaccgcg aaacatcgtg gccatttgtg gctgaatagc gtcgagcatc tcatagcgcc
     1981 gacggtattc agcccgtttt ttactggcga tttcggcaat ctcttttcgt gctatctgtg
     2041 ctggaaggcg gtaatggcgt tcagcatcac atacgccgcc aaccgattcc cagaaagcgt
     2101 tgtaatcagc gtggatcttg ccttctttat cgcgataacg caggctgcgg taaatatgcg
     2161 tttcattgct gacggcaata atctgctcta cctgcaaacg ttgggcaaac agacaggccg
     2221 cttccatcac gaggcgtttg ggaaatagcc cgtggcaggc tttcgtcgca ttctggattt
     2281 cctgatgtgg aatttcccat tttgcgcctt gcagtccgcc aataaacatc gttcttttcc
     2341 cctgatattc acacagggta aacgtgatct ctgccagagg aataccttcg ctgttgcgaa
     2401 acaggattgt gctgtcacct tctttatcca ttgagatcat catggtcagc tcaagcgtga
     2461 actgctcgcc gtttttgcct tccagcttcg ccagttgcag cccgggggta ttcaaatata
     2521 agctgaattc ttccgccgac atacatccgc ggagtaacgc ataatggtaa cgtaacgcct
     2581 ccagcaattg cttacggcta agattcgccg caaggtaagg gcgatgcaga cgcacaggca
     2641 gtcgcggttg gcgcgttaac aacacattga gattaggcca gtgggaaagt tcgttcatcc
     2701 actcaacgct taaacgcggc ataatcaacg agcgcagcaa aaatttctgg cgaaaactac
     2761 ggcgatgcca gaatttaccc ggccgacact gtccacgtgc cagactaaga aaaagtgaca
     2821 ggctgctgag agattcagat ggcgtaaagg tccgttcagt tagctgcgac atattcatga
     2881 aatcaatggt tatacatgac gtcgatttca ccattgcgta tcttaaccaa acatcaatag
     2941 tgtgattact aacgtaaatt ttatggtttt gttgatattt cgttgaagtt aatgacccgg
     3001 attggcatat ggagtattca gaatatttat gaaaaagcgg aaaaccgtga agaagcgtta
     3061 cgttattgcg ctggtgatag tcatcgccgg actgattacg ttatggagaa ttcttaacgc
     3121 acccgtgccg acttatcaga cactgattgt gcgccccggt gatttacagc aaagcgtgct
     3181 ggcgaccgga aagctggacg cgctgcgtaa ggttgacgtg ggcgcgcagg tcagcggtca
     3241 gttgaaaacg ctgtcggtgg cgattggcga taaagtaaaa aaagaccagc ttttaggggt
     3301 cattgatcct gaacaggctg aaaaccagat caaggaggtc gaagcaacgc tgatggagct
     3361 acgcgcgcag cggcagcagg cggaagcgga gctgaaactg gcgcgggtga cgtattcccg
     3421 tcagcaacgt ctggcacaaa cgcaggcggt ttcacagcag gatctcgaca ccgccgcgac
     3481 ggagatggct gtgaaacagg cgcaaattgg caccattgac gcgcaaatca agcgcaatca
     3541 ggcttctctc gatacggcta aaaccaatct cgattacact cgcatcgttg ccccgatggc
     3601 cggggaagtc acgcaaatca ccactctgca aggccagacg gtgattgccg cacaacaagc
     3661 accgaacatt ctgacgctgg cagatatgag caccatgctg gtaaaagcgc aggtttctga
     3721 agcggatgta atccacctga agccggggca aaaagcctgg tttacggtac ttggcgatcc
     3781 actgacgcgc tacgaggggc aaatcaagga tgtactaccg acgccggaaa aggttaacga
     3841 cgctattttc tattacgccc gttttgaagt ccccaacccc aatggtttgc tgcggctgga
     3901 tatgactgcg caagtgcata ttcagctcac cgatgtgaaa aatgtgctga cgatccctct
     3961 gtcggcgtta ggcgatccgg ttggcgataa tcgttataaa gtcaaattgt tgcgtaatgg
     4021 cgaaacacgc gagcgtgaag tgacgattgg cgcacgtaac gataccgatg ttgagattgt
     4081 caaagggctg gaagcgggcg atgaagtggt gattggtgag gccaaaccag gagctgcaca
     4141 atgacgcctt tgctcgaatt aaaggatatt cgtcgcagct atcctgccgg taatgagcag
     4201 gttgaggtgc tgaagggcat cagcctcgat atttatgcgg gtgagatggt cgcgattgtt
     4261 ggcgcttcgg gttccggtaa atcgaccctg atgaatattc tcggctgcct ggataaggcc
     4321 accagcggca cctatcgcgt agccggtcag gatgttgcca cgctggacgc cgatgcgctg
     4381 gcgcaactgc gccgcgagca tttcggcttt attttccagc gttaccattt gctttcgcat
     4441 ttaaccgccg agcagaacgt tgaagtaccc gccgtctatg ctggtcttga gcggaaacag
     4501 cgactgcttc gtgcccagga gttgctgcaa cggctggggc tggaagaccg tacagagtat
     4561 tatccggcac agctttcggg tggtcagcaa cagcgcgtca gcatcgcgcg ggcattgatg
     4621 aacggcggtc aggtaattct tgccgatgaa ccaaccggcg cactggacag ccattctggc
     4681 gaagaggtga tggcgatcct gcatcagctg cgcgatcgcg ggcatacggt gattatcgtc
     4741 acccacgatc cgcaggtcgc tgctcaggcc gagcgggtga tcgaaattcg cgacggcgaa
     4801 attgtgcgca atcctcccgc cattgaaaaa gtgaatgttg ctggcgggac ggagcctgtt
     4861 gtcaatacag tgtctggctg gcggcagttt gtcagcggtt ttaacgaggc gctgacgatg
     4921 gcatggcggg cgctggcagc gaataaaatg cgtactttac tgaccatgct ggggattatt
     4981 atcggtattg cgtcggtggt ttccattgtc gtggtgggtg acgccgccaa acaaatggtg
     5041 ctggcggata ttcgttctat tggtacgaat actattgatg tctatcccgg gaaagatttt
     5101 ggcgatgacg atccgcaata tcagcaggcg ctgaagtacg acgacttaat cgccatccaa
     5161 aaacaaccgt gggtcgcctc agccacacct gccgtctcgc aaaacctgcg cctgcgttat
     5221 aacaatgttg atgttgctgc cagtgccaat ggcgtgagcg gcgattattt taatgtctat
     5281 ggcatgacct tcagtgaagg aaacaccttt aatcaggagc agctgaacgg tcgtgcgcag
     5341 gtcgtggttc tcgacagtaa tactcgccgc cagcttttcc cccataaagc agatgtggtt
     5401 ggcgaggtga ttctggtcgg caatatgccc gccagagtca ttggtgtggc ggaagaaaaa
     5461 cagtcgatgt ttggtagcag taaagtgctg cgtgtctggc taccttacag cacgatgtcc
     5521 gggcgagtta tgggccagtc gtggcttaac tccattactg tcagggtgaa agaaggattt
     5581 gacagcgccg aggcggaaca gcaactcacg cgtttacttt cactgcgcca cggtaagaag
     5641 gatttcttta cctggaacat ggacggcgtc ttgaaaactg ttgaaaagac cacacgtact
     5701 ttacaactgt ttctgacgct ggtggcggtg atttcgctgg tggtgggtgg tattggtgta
     5761 atgaatatta tgctggtgtc agtgaccgag cggacgcggg aaattggcat tcgcatggct
     5821 gtaggtgcgc gagcaagcga tgttttgcaa cagttcctga tcgaagccgt actggtttgc
     5881 ctggtcggtg gcgcgttggg aataactctg tcactgttaa ttgctttcac cttgcagctt
     5941 ttcttacccg gctgggagat tggtttttca ccgttggcac tgctgctggc gtttctctgc
     6001 tcgacggcca ccgggatttt atttggatgg ttacccgcac gaaatgcggc acgactggat
     6061 ccagtagatg ctctggcacg agagtaattt ttgagataaa aatgccagcc gatcgggctg
     6121 gcattttgcc tttaggatgt acacaatgag acagaagagc tatgcgactg ccgcttctac
     6181 ttcgacgggc acaataacac tggcgtgatt gccttttggc ccctggtgga catcaaactg
     6241 aacggattgt ccagctttta gcgttctata accatccatc tgaatggtgg aataatgagc
     6301 gaaaatatct tcgccgccgc cttcagggca gatgaaacca aaccctttgg cattgttgaa
     6361 ccacttaaca gtaccctttt ccatgcttcg acatccttcg caaatcttat acaagtaaga
     6421 tggaataaac cggggtcaga gagggggctg ttcaaaacct cgccaactct agaaatacaa
     6481 tttagagaat tagggcgagc cgtcaagcat ttgacagggg ccagggggca ggtatgaatc
     6541 aaaaatttga agcagttaac gctattgaca ggaatgtgac agatgtcgct gatgccaacg
     6601 atagatgata gttatttatc atctagtgtt cgcgggtgtt cgtggtagtg ttttgtgtgc
     6661 attcatcgct atgattataa aggaaaattt tgttttcctt gttcgctctt gttcgtcacg
     6721 gtttgccctt gtacgatgca cggtgtagtc aatggtgtag ttaattttga tgaccggaaa
     6781 cgcgaaacat gagcagcgca attcatagac ttagcgacac tcttttacga aaattaagcg
     6841 gatcaccaac cacaaaaaac gcctttttta atgacggtgg caatcttagc gtaagacatt
     6901 ccaccagcgg cctgttaacc tggtatttca cttacagggc cggaactggc aggcaggtat
     6961 caccggaacg cctgagactg ggcaactatc ccgatctgag cctgaaagca gccagggaaa
     7021 aagcggcaca gtgtcgcgcc tggctggctg aggggaaaaa tccgcgctat gagcttaatc
     7081 gtgctgtaca ggatgcgtta gcccccgtga cagttaagga agcgctcact tactggcttg
     7141 aatcgtatgc aaaggaaaag cgcactgact acgaatcact gaaaagccgg atcaataaac
     7201 acataatcag ccagattggc gctttgccgt tggaaaaatg cgagctacgg cactggttgg
     7261 catgttttga ccagatggca aagcgaagcc cagtatctgc cggatttttg ctacaggtat
     7321 gcaaacaggc gcttaagtac tgccgaaaac gacgatacgc aatcagcaac gttcttgatg
     7381 atatggttgt cggtgatgtg ggaaaaaaag cagaaataag cgagcgcgta ctaacaaaca
     7441 aggagcttgg ggaattactc cgcgccctgg atgaaaaaat ttacccaccg tactacagcg
     7501 ccctgattcg cctcctgatt gtcttcggat gccgtgccac tgagctaaga cgctctgagg
     7561 tccaggaatg ggattttaaa gaaatgctct ggaccgtgcc gaaagaacac agcaaaacga
     7621 aggtagcaat attcaggcca ataccggaag cgatcttgcc gttcgtaacg aagctggtgg
     7681 agcagaacag gcacacggga ttattgctcg gagaactgaa agggcaatca tccgtatcag
     7741 agtacggaag aacggcacac agacgcatta atcaagcccc ctggacgttg cacgacatcc
     7801 ggcacacgtt tacaacgatg ctgaacgatt taggcgtgga tcctcatgta gtggagcagc
     7861 taaccgcgca ccagttgcca ggagtgcaac gagtctataa tcactcccgt tatctcgatg
     7921 caaaacgtga cgctcttaat ctatgggttg agcgtctcga gcttctccag aacaatgatg
     7981 aaaaaatcgt tgttatgacc ccgcgaattt actcccaaaa ttcttgacaa attacggctg
     8041 tttttcttgc gaaaaatggc cctctaaaat aaatcgtcga atatcggcaa atatcgacga
     8101 tttatatgtg tcaactatcg aaaaaaattg aaatgtcttc ttctgagttc ttattctctg
     8161 tttcttgatt attccaatac ctgatggttt atctatttgt ttttaatgag gtaattcagg
     8221 tgtcatgcca aatggcgaca ttttttgttg cgtcgcttaa aaactcgtga cacgatttta
     8281 aacactaacg agcttctacg aaccttagcg aacaaagcct ttagca
//
LOCUS       DAEEKQ010000165         8321 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.167, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000165 DAEEKQ010000000
VERSION     DAEEKQ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8321
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.167"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            292..1269
                     /gene="csiD"
                     /locus_tag="KLK57_003485"
     CDS             292..1269
                     /gene="csiD"
                     /locus_tag="KLK57_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HBE0893762.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1289..2557
                     /gene="lhgO"
                     /locus_tag="KLK57_003486"
     CDS             1289..2557
                     /gene="lhgO"
                     /locus_tag="KLK57_003486"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HBE0893763.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSKHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2580..4028
                     /gene="gabD"
                     /locus_tag="KLK57_003487"
     CDS             2580..4028
                     /gene="gabD"
                     /locus_tag="KLK57_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HBE0893764.1"
                     /translation="MKLNDSNLFRQQALINGEWLDANNGGIIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERANILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4042..5322
                     /gene="gabT"
                     /locus_tag="KLK57_003488"
     CDS             4042..5322
                     /gene="gabT"
                     /locus_tag="KLK57_003488"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HBE0893765.1"
                     /translation="MNSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEHENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5633..6949
                     /locus_tag="KLK57_003489"
     CDS             5633..6949
                     /locus_tag="KLK57_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HBE0893766.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSA
                     LYTASRMLYSLSRRGDAPAVMGKINRSKTPYVAVLLSTGAAFLTVVVNYYAPAKVFKF
                     LIDSSGAIALLVYLVIAVSQLRMRKILRAEGSEIRLRMWLYPWLTWLVIGFITFVLVV
                     MLFRPAQQLEVISTGLLAIGIICTVPIMARWKKLVLWQKTPVHNTR"
     gene            6970..7632
                     /gene="csiR"
                     /locus_tag="KLK57_003490"
     CDS             6970..7632
                     /gene="csiR"
                     /locus_tag="KLK57_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HBE0893767.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7633..8082)
                     /gene="kbp"
                     /locus_tag="KLK57_003491"
     CDS             complement(7633..8082)
                     /gene="kbp"
                     /locus_tag="KLK57_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HBE0893768.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8166..>8321)
                     /locus_tag="KLK57_003492"
     CDS             complement(8166..>8321)
                     /locus_tag="KLK57_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HBE0893769.1"
                     /translation="GFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIHA
                     FWVQTRD"
BASE COUNT         1867 a         2182 c         2336 g         1936 t
ORIGIN      
        1 ttagtttact aacggtcatt ttgcagtgaa gccatttact gttttttatc gaccagataa
       61 tctgttctct aatgttaact ccccctaacc tgttgcttta gttattcatt tcctgtctca
      121 ctttgcctta atactctacg ttaaatgtta ctaatttgtt gcttttgatc acaataagaa
      181 aacaatatgt cgcttttgtg cgcatttttc agaaatgtag atatttttag attatggcta
      241 cgaaatgagc atcgccatgt caccctacat ctcataagag gatcgcttct gatgaatgca
      301 ctgaccgccg tacaaaataa cgctgtcgat tcaggccagg actatagcgg attcactctc
      361 atcccgtcgg cgcaatcccc gcgtctgctg gaactcacct tcaccgaaca gacgaccaaa
      421 cagtttctcg agcaggttgc cgaatggccc gtacaggcgc tggagtacaa atcgtttctg
      481 cgttttcggg taggcaaaat tcttgacgat ctgtgtgcga atcagctgca accattgctg
      541 ttgaagaccc tgctaaaccg tgctgaaggt gcgctgttga tcaatgcggt gggtatcgat
      601 gatgtggcgc aggcagatga gatggtgaag ctggcaacgg cggtagcgca tctgattggt
      661 cgctcaaatt tcgatgcgat gagcggtcag tattacgcgc gtttcgtggt gaaaaatgtc
      721 gataactcag acagctatct gcgtcagccg caccgcgtga tggaactgca caacgacggc
      781 acgtatgtcg aagagatcac cgattacgtg ctgatgatga aaatcgatga gcaaaacatg
      841 cagggtggaa attcgttgct gctgcatctc gatgactggg aacatctgga ccactatttc
      901 cgccatccac tggcgcgtcg cccgatgcgc tttgccgcgc cgccgagcaa aaacgtcagc
      961 aaagatgttt tccatccagt gttcgacgtt gatcaacagg gtcgcccggt gatgcgctat
     1021 atcgaccagt tcgtccagcc aaaagatttt gaagaaggcg tgtggttgag cgagctttcc
     1081 gatgccattg aaaccagcaa aggcattctt tctgtacccg ttcccgttgg caaattcctg
     1141 ttgataaaca acctgttctg gctgcacggt cgcgaccgct ttactccgca cccggatctg
     1201 cgccgtgaac tgatgcgtca gcgtggctat ttcgcttacg ccactcacca ctaccagacg
     1261 catcagtaag cgcaaaggaa ttgagcggat gtatgatttt gtgattattg gcggcggcat
     1321 catcggcatg tcgaccgcca tgcaactgat tgatgtttat ccggatgccc gcattgcgtt
     1381 gctggaaaaa gagtccggcc cggcctgtca ccagacgggc cacaacagcg gcgtgatcca
     1441 tgccggggtc tattacacgc ccggcagcct gaaggcgcag ttttgcctgg cggggaaccg
     1501 cgccaccaaa gccttttgcg atcaaaacgg cattcgctat gacaactgcg gcaagatgct
     1561 ggtcgccacg tctcagctcg aaatggaacg gatgcgcgcc ttatgggaac gcacagcggc
     1621 gaacggtatc gagcgcgagt ggataaacgc cgtggaactg cgcgagcgcg aaccgaatat
     1681 caccgggctt ggtggcattt ttgtgccgtc cagcggcatt gtcagctacc gcgaagtcac
     1741 agcggcaatg gcaaaaatct tccaggccag aggcggcgag attatttata acgccgaagt
     1801 cagcgccctc agtaagcata aaaacggcgt ggtgatacgc acccgtcagg gcggtgaata
     1861 tgaagcatcg acgctgatta gctgttccgg gctgatggct gaccggctgg tgaaaatgct
     1921 cggcctcgaa ccgggcttta tcatctgccc gttccgtggt gagtatttcc gccttgcgcc
     1981 ggagcataac cagattgtta accacctgat ttaccccatt cccgaccccg cgatgccatt
     2041 tttgggcgtg catctcaccc gcatgatcga cggcagcgtg accgtcgggc caaacgcggt
     2101 gctggctttc aaacgcgaag gctatcgcaa gcgcgacttc tcatttagtg acacgctgga
     2161 gattttgagc tcgtcgggga ttcgccgggt gctgcaaaac catctacgct caggactggg
     2221 cgagatgaaa aactcgctgt gcaaaagcgg ctacctgcgg ctggtgcaaa agtattgtcc
     2281 ccggctttcg ttaagcgatc tccagccctg gcccgccggt gtgcgggcgc aggcagtatc
     2341 gccggacggc aagctgattg acgattttct gtttgtcacc accccacgca cgatccacac
     2401 ctgcaatgcg ccctccccgg cagcgacatc agcaattcct attggtgcgc atattgtcag
     2461 caaggtacaa acgctgttgg caagccagag taaccccgga cgcacgctgc gagcggcacg
     2521 tagtgtggat gccttacacg ccgcatttaa tcaataacct ttgaaaacag gacgtaacaa
     2581 tgaaacttaa cgacagtaac ttattccgcc agcaggcgtt gattaacggg gaatggctgg
     2641 acgccaacaa tggcgggatc atcgacgtca ccaatccggc gaacggcgac aagctgggta
     2701 gcgtgccgaa aatgggcgct gatgaaaccc gcgccgctat cgacgccgcc aaccgcgccc
     2761 tgcccgcctg gcgtgcgctc accgccaaag aacgcgccaa cattctgcgc aactggttca
     2821 atttgatgat ggagcatcag gacgatttag cgcgcctgat gaccctcgaa cagggcaaac
     2881 cactggccga agcgaaaggc gaaatcagct acgctgcctc ttttattgag tggtttgccg
     2941 aagaaggcaa acgtatttat ggcgatacca ttcccggtca tcaggccgat aagcgcctga
     3001 ttgttatcaa gcagccgatt ggcgttaccg cagctatcac gccgtggaac ttcccggcgg
     3061 cgatgattac ccgtaaagcc ggtccggcgc tggcggcagg ctgcaccatg gtgctgaagc
     3121 ccgccagtca gacaccgttc tctgcgctgg cgctggcgga gctggcgatt cgcgcgggca
     3181 ttccggctgg cgtgtttaac gtagtcaccg gttcggcagg cgcggtcggt aatgaactga
     3241 ccagcaaccc gctggtacgc aaactgtcgt ttaccggttc gaccgaaatt ggccgccagt
     3301 taatggaaca gtgcgcgaaa gacatcaaaa aagtgtcgct ggagctgggc ggcaacgcgc
     3361 cgtttatcgt ctttgacgat gccgatctcg ataaagccgt ggaaggcgcg ctggcctcga
     3421 aattccgcaa cgccgggcaa acctgcgtct gcgccaaccg tttatacgtg caggacggcg
     3481 tgtatgaccg ctttgccgaa aaattgcagc aggcggtgag caaactgcac atcggcgacg
     3541 ggctggataa aggcgtcacc atcgggccgc tgatcgatga aaaagcggta gcaaaagtgg
     3601 aagagcatat tgccgatgcg ctggagaaag gtgcgcgtgt ggtttgcggc ggtaaagcgg
     3661 atgaacgcgg cggcaacttc ttccagccga ccattctggt ggacgtgccg gccaacgcca
     3721 aagtgtcgaa agaagagacg ttcggccccc tcgccccgct gttccgcttt aaagatgaag
     3781 ccgatgtgat tgcgcaagcc aatgacaccg agtttggcct tgccgcctat ttctacgccc
     3841 gtgatttaag ccgcgtcttc cgcgtgggcg aagcgctgga gtacggcatc gtcggcatca
     3901 ataccggcat tatttccaat gaagtggccc cgttcggcgg catcaaagcc tcgggtctgg
     3961 gtcgtgaagg ttcgaagtat ggcatcgaag attacttaga aatcaaatat atgtgcatcg
     4021 gtctttaact ggagaatgcg aatgaacagc aataaagagt taatgcagcg ccgcagtcag
     4081 gcgattcccc gtggcgttgg gcaaattcac ccgattttcg ctgaccgcgc ggaaaactgc
     4141 cgggtgtggg acgttgaagg ccgtgagtat cttgatttcg cgggcgggat tgcggtgctc
     4201 aataccgggc acctgcatcc gaaggtggtg gccgcagtgg aagcgcagtt gaaaaaactg
     4261 tcgcacacct gcttccaggt gctggcttac gagccgtatc tggagctgtg cgagattatg
     4321 aatcagaagg tgccgggcga tttcgccaag aaaacgctgc tggttacgac cggttccgaa
     4381 gcggtggaaa acgcggtaaa aatcgcccgc gccgccacca aacgtagcgg caccatcgct
     4441 tttagcggcg cgtatcacgg gcgcacgcat tacacgctgg cgctgaccgg caaggtgaat
     4501 ccgtactctg cgggcatggg gctgatgccg ggtcatgttt atcgcgcgct ttatccttgc
     4561 ccgctgcacg gcataagcga ggatgacgct atcgccagca tccaccggat cttcaaaaat
     4621 gatgccgcgc cggaagatat cgccgccatc gtgattgagc cggttcaggg cgaaggcggt
     4681 ttctacgccg cgtcgccagc ctttatgcag cgtttacgcg ctctgtgtga cgagcacggg
     4741 atcatgctga ttgccgatga agtgcagagc ggcgcggggc gtaccggcac gctgtttgcg
     4801 atggagcaga tgggcgtggc gccggatctt accacctttg cgaaatcgat cgcaggcggt
     4861 ttcccgctgg cgggcgtcac cgggcgcgcg gaagtgatgg acgctgttgc accgggcggt
     4921 ctgggcggca cctatgccgg taacccgatt gcctgcgtgg ctgcgctgga agtgttgaag
     4981 gtgttcgagc atgaaaatct gctgcaaaaa gccaacgatc tggggcagaa gctaaaagac
     5041 ggattgctgg cgatagccga aaaacacccg gagatcggcg acgtacgcgg gctgggagcg
     5101 atgattgcca tcgagctgtt tgaagatggc gatcacagca aaccggacgc aaaactcacc
     5161 gccgagatcg tggcacgcgc ccgcgataag ggcctgattc ttctctcctg cggcccgtat
     5221 tacaacgtgc tgcgcatcct tgtaccgctc accattgaag acgctcagat ccgtcagggt
     5281 ctggagatca tcagccagtg ttttgctgag gcgaagcagt agctatgtcc gtttgtaaga
     5341 gttaaccgtt gctaagggaa tgataacgat gcagtaaaaa ataataacga tgaggcgaac
     5401 gacaaagtta aaaacgactg cggacagtcc cctcgcccct ctggagagag ggttagggtg
     5461 aggggtaaaa cacgcggcta aaccctcccc tggccctctc cctaagggag agggagaaaa
     5521 ccctcccgct ttcgttttta agattttgtt gaccgtctca attaaacgcg tgacccggcg
     5581 cgggaaatgt cggggcgctc tcccaagtga cacactttcg agaggattca ggatggggca
     5641 atcatcgcaa ccacatgagt taggcggcgg gctgaaatca cgccacgtca ccatgttgtc
     5701 tattgccggt gttatcggcg caagtctgtt tgtcggttcc agcgtcgcca tcgccgaagc
     5761 gggcccggcg gtattactgg cctatctgtt cgccgggcta ctggtggtta tgattatgcg
     5821 gatgttggcg gaaatggcgg ttgccacgcc cgataccggt tcgttttcca cctatgccga
     5881 taaagccatt ggtcgctggg cgggctatac catcggctgg ttgtactggt ggttttgggt
     5941 actggttatc ccgctggaag ccaatatcgc cgctatgatc ctgcactcgt gggttccagg
     6001 cattcccatc tggttatttt ccctcgtcat tacccttgcc ttaactggca gtaatttatt
     6061 aagcgttaaa aactacggcg aatttgagtt ctggctggcg ctgtgcaaag tcatcgctat
     6121 cctggccttt attttccttg gtgcagtcgc aattagcggt ttttaccctt atgccgaagt
     6181 gagcgggatc tcaagattgt gggatagcgg cggctttatg cccaacggtt tcggtgcggt
     6241 attaagcgcg atgttgatca ccatgttctc gtttatgggc gcagaaattg tcaccattgc
     6301 cgccgcggaa tccgacacgc cggaaaaaca tattgtccgc gccaccaact cggttatctg
     6361 gcgtatttct atcttctatt tgtgctctat ttttgtcgtc gtggcattaa taccgtggaa
     6421 tatgccgggg ctgaaagccg ttggatctta tcgctcggcc ctgtataccg cctcaaggat
     6481 gctctactcc ttaagccgtc gcggtgatgc tcccgcggta atgggtaaaa tcaaccgcag
     6541 taaaaccccg tacgtggcgg tgttactctc caccggcgcg gcattcttaa cggtggtggt
     6601 gaactattac gcgcctgcga aggtatttaa atttctgatc gacagctccg gcgctatcgc
     6661 cctgctggtt tatttagtca tcgccgtttc acagttgcgg atgcgcaaaa ttctgcgagc
     6721 ggaaggaagc gaaattcgct tgcgaatgtg gctttatccc tggctcacct ggctggtcat
     6781 cggctttatt acctttgtgt tggtagtgat gctattccgt ccggcgcaac agttagaagt
     6841 gatctccacc ggcttattag cgatagggat tatctgtacc gtgccaatta tggctcgctg
     6901 gaaaaagctg gtattgtggc aaaaaacacc cgttcataat acgcgctgat aatgatcagg
     6961 agtcacacca tgaccattac gtctctggat ggctatcgct ggctgaagaa cgatattatt
     7021 cgcggtaatt ttcaaccgga tgaaaaatta cggatgagtt tgctgacatc gcgttatgca
     7081 cttggcgttg ggccgttgcg ggaagcactt tctcaactgg tggcggaacg gctggtcacg
     7141 gtggtgaatc aaaaagggta tcgggtggcg tctatgtcag agcaggagct gctcgatatt
     7201 ttcgatgccc gcgccaatat ggaagcgatg ttagtgagtc tggcgattgc ccgcggtggc
     7261 gatgagtggg aggcagacgt tctcgcaaaa gcgcatctgc tcagtaagct ggaagcctgt
     7321 gacgccagcg agaaaatgct tgatgagtgg gatctgcgtc atcaggcgtt tcatacagca
     7381 attgtggcgg gctgtggttc tcactatttg ctgcaaatgc gtgaacggtt gtttgatctg
     7441 gcggcgcgct atcgatttat ctggctgcgg cgaacggtgc tttcggtaga aatgctggag
     7501 gataaacacg atcagcacca gaccctgact gcggcggtac tggcgcgaga taccgcgcgc
     7561 gccagtgagt taatgcgcca gcatttactg acgccaattc ccattatcca gcaggcgatg
     7621 gctggcaatt aactactctt ccggaatacg caacacttgc cccggataaa ttttatccgg
     7681 gctttttagc atcggtttat tcgcttcgaa gattttattg tacagattag cgttaccgta
     7741 gacctgtttg gaaatggcac tcagagtgtc gccagactta acggtataaa actggctggc
     7801 agtggctggt gtcgccgttt tcacctgatc atcaacactg gcaataccgg aaatattccc
     7861 caccgcaaca aggattttct ccttcgcctc ctgactcagg ccgtcaccag tgaccgtcgc
     7921 tttgccgtcg gcaatttgaa tgttcacttt atcagcatca ggtataccgg ttttgctcag
     7981 atgctcctgc accttcttcg cctgatcgtc tttatcgtgc tgacctgtaa ccgcgtccca
     8041 gagtttttct ccggcatctt tcacaaaatt gaacagaccc atagctacct cattcgttaa
     8101 cggaaacact cagaaagtgt agcagaggaa cgcggcgcgt taacgccgcg ggggtgagca
     8161 caggtttaat cgcgggtttg cacccagaac gcgtgaatca aaccggggat atacccaagc
     8221 agtgtcagca gaatattaat aatgaacgcc caaccgaacc ctttaccgag cagcacgccg
     8281 agcggcggta gaatgatggt gatgacgatt ctccagaaac c
//
LOCUS       DAEEKQ010000166         8296 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.168, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000166 DAEEKQ010000000
VERSION     DAEEKQ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8296
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.168"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            500..1771
                     /locus_tag="KLK57_003493"
     CDS             500..1771
                     /locus_tag="KLK57_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HBE0893770.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVTIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            complement(1801..2805)
                     /gene="dppF"
                     /locus_tag="KLK57_003494"
     CDS             complement(1801..2805)
                     /gene="dppF"
                     /locus_tag="KLK57_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HBE0893771.1"
                     /translation="MSTQEATLQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLSGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(2802..3785)
                     /gene="dppD"
                     /locus_tag="KLK57_003495"
     CDS             complement(2802..3785)
                     /gene="dppD"
                     /locus_tag="KLK57_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HBE0893772.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            complement(3796..4698)
                     /gene="dppC"
                     /locus_tag="KLK57_003496"
     CDS             complement(3796..4698)
                     /gene="dppC"
                     /locus_tag="KLK57_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HBE0893773.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYIVIVLF
                     ITIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            complement(4708..5727)
                     /gene="dppB"
                     /locus_tag="KLK57_003497"
     CDS             complement(4708..5727)
                     /gene="dppB"
                     /locus_tag="KLK57_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HBE0893774.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            complement(6035..7642)
                     /gene="dppA"
                     /locus_tag="KLK57_003498"
     CDS             complement(6035..7642)
                     /gene="dppA"
                     /locus_tag="KLK57_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HBE0893775.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQTPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            complement(7994..>8296)
                     /locus_tag="KLK57_003499"
     CDS             complement(7994..>8296)
                     /locus_tag="KLK57_003499"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893776.1"
                     /translation="VGLIRRASVASGNGTAFIVGCGATALFDQHTTHFPANFAPHISL
                     SINGFVTETIVFFYICLNLFLRYLWLALAFSAVRFSVHGINRSDIHLFLRFFSLNA"
BASE COUNT         1912 a         2250 c         2096 g         2038 t
ORIGIN      
        1 catgcccagc tctgcgagcg tcatatagcc gcctgtgttg taatgacaac gtttcgcggc
       61 tattcttgag tggtctaggt tcaagattag cccccgttat gttgtcaggt acatacctgc
      121 aacgtgcggg ggttttctct ctccagcaac caatgccacc agggataaag cccccgcaac
      181 attgcgcctc accggaaccc tttcggcttg ccgctgattt tacgattgct ttttactgtc
      241 agcacccggc aatcttcctc attgcgcgat gccttccagc ttaatttctt tatttgcaat
      301 agttgcagtt catatccggg ctgactccag actggtagtg aaatgcgcaa ttttgtagaa
      361 ttgcgcctcc tcgcgacctc cccagtgaat ctgttgcctc attttttaca tactgcgtat
      421 tcgacttctc cacctgttgc gcaagagaaa ctgggtttat tcatttttgc gaggccgact
      481 tctttctgga caggacttta tgcagcacaa cacactatcg aaacacaatc agaaattgcc
      541 gtttacacgc tacgacttcg gctgggtttt attatgcata ggcatggcga ttggtgccgg
      601 aaccgtgctg atgccagtac aaattggctt gaagggaatt tgggtattta ttaccgcagc
      661 gatcattgct tatcctgcca cctgggtagt gcaggacatt tatttaaaaa ccctttctga
      721 aagcgattcc tgtaatgact acaccgatat tatcagtcat tacctgggga agaactgggg
      781 aattttcctc ggggttatct actttttgat gattatccac gggattttta tctactctct
      841 ctccgtggtt ttcgacagcg cctcgtacct gaaaaccttc ggtttaaccg atgccgatct
      901 ttcacaatct ctactttata aagtcgctat tttcgccgta ctggtaacga ttgcgtctgg
      961 tggtgaacga ttactgttta agatttccgg gccaatggtg gtggtcaaag tagggattat
     1021 tgtcgtgttc ggttttgcga tgatcccgca ctggaatttc gccaatataa ccgccttccc
     1081 gcaagcctcc gtctttttcc gcgatgtctt gcttaccatt ccattttgct tcttttctgc
     1141 agtatttatt caggtactta acccaatgaa tattgcctat cgtaaacggg aagcggataa
     1201 agtactggca acccggctcg cgctgcgtac ccaccgaatt agttatatca cgctcatcgc
     1261 ggtgatcctg ttttttgcct tttcgtttac cttctcaatt agccacgaag aagccgtttc
     1321 tgcctttgaa caaaatatct cagcactggc gctggccgcg caggtgatcc ctgggcatat
     1381 cattcatatc acctctacgg tgcttaatat ctttgccgta ctgaccgcat tctttggcat
     1441 ttatctcggt ttccacgagg ccattaaagg cattattctc aatctgttaa gccgaattat
     1501 tgataccaag aaaattaact cacgcgtgct gactctggcg atctgcgctt ttatcgtcat
     1561 tacgttgacg atttgggttt cgtttcgtgt atcggtgctg gtgttctttc agttgggaag
     1621 cccgttatat ggtattgtgt cgtgcctcat tccgtttttc ctgatctata aagtcgcaca
     1681 actggaaaaa cttcgcggat ttaaagcctg gctgattctg ctgtacggca ttttgctatg
     1741 cttgtcgcca ctgttgaagc tgattgagta aaccggagcg catggccccg gttttgtgag
     1801 ttaacgctgc ggattttcat cctgatcaac agcaaaacaa gctaccagtt gaccgccgta
     1861 gtcttttagc tgcggctgca actgggtgca ggggccgaag cgccgacgac agcgggcgtt
     1921 gaaggcgcaa cccggcggtg gattcagtgg gcttggtagt tcaccgctga gcttgatgcg
     1981 ctcgcggcga tcgtccgggt tcaggcgcgg cgtcgcggaa agtagcgcct gagtgtacgg
     2041 atggcgcggg ttattgaaga tttggtcttt cgttcccttc tccacgcagc ggcccaggta
     2101 catcaccatc acttcatcag caatgtgctc caccaccgac aggtcgtggg agataaagac
     2161 ataagacagc cccaactcct gctgcaaatc catcatcaga ttcagcacct gcgcgcgcac
     2221 tgaaacatcc agcgcggaaa ccggttcatc ggcaatcacc acatccgggt cgagcatcag
     2281 accacgggcg atggcgatac gctgacgctg accgccggag aacatatgcg gatagcggtc
     2341 atagtgctcg gttttcaggc cgactttcgc catcatcgac agggcttttt cccgacgctg
     2401 ttctttgctt aagctggtgt tgatcagcag cggctcttca agaatttgcc cgactttttt
     2461 acgcggattc agcgaaccgt aagggttctg gaagacgatc tggattttct gccgacgcag
     2521 cttctgcgcc tgcggatcgt gcttaagcag atcctgcccc tggtaataca gctcgccacc
     2581 ggtgggcgtt tcaatcatcg tcagcaaccg accgagggtc gatttaccgc agccagattc
     2641 gcccactact gccagcgttt tgccacgttc aaggttaaac gaaacgccat ccagcgcttt
     2701 aaccagacgt tccggcgcga acatgccttt cttcaccgga taatgttttt tcaggtcgat
     2761 agcctgcaac agcggttgtt gcagggtggc ctcttgcgta ctcatagtgt cggcctcccg
     2821 gcatcatcaa gtgggtaatg gcatttggac tggcgcccgt cagcgagcat attcagcgcc
     2881 ggttcttcag cgcgacatct gtcagtggca taggggcagc gcgggttaag caggcagccg
     2941 ttcgggcggt cgtacttgcc gggaacgaca cctggcaacg acgccagacg ttctttgtcc
     3001 tgagcaaatt ctggcagcgc acgcagcaat gcctgagtat acgggtgacg cggcgcatgg
     3061 aagatggcgt gcgcatcgcc cgtttccacc acctggcctg catacatcac aatgatttta
     3121 tgcgcagctt ccgccaccag cgccagatca tgggtaatta acaccagcgc catgttctct
     3181 ttctgctgta gctccagcaa cagttcgatg atttgcgcct gaatggtcac gtccagcgca
     3241 gtggtcggct catcggcaat cagcagtttt ggccgacagg caatcgccat ggcgatcatc
     3301 acgcgctggc tcatgccgcc ggaaagctga tgcgggtaaa catccagacg cgatgccgga
     3361 tcgggaatac cgacctgatt cagcaggtca atcgcccgct gacggcgagt gctcttatta
     3421 ccgccctgat gcaccttaat cgcttccatg atctggaaac ccacggtgta gcacgggtta
     3481 aggctggtca tcgggtcctg gaagatcatc gccacttcgg cacccaccag gttgcggcgc
     3541 tctttttctg atatacgctg caaatcctga ccgttaaaca ccagcttttc cgccattacg
     3601 cggcccggat aatcaatcag ccccataatc gccagtgaac tgaccgactt accggagcca
     3661 gactcaccca caatcccgac cacttcgccc tgttttacgc tgtagctgat gcggtctacg
     3721 gcgcggaacg gtgcgctttc gtcgccgaaa tgcaccgata atttatctac atttaataac
     3781 gccatctcga acctcttact gcttcagttt gggatcgagc gcgtcacgca gaccgtcacc
     3841 catcaggtta aatgccagca ccgtcagcag gatcgccaga cccgggaagg tcacgaccca
     3901 ccaggcgctt tgcgcgaact gcaacacgtc ggagagcatg gtgccccact ctggtgttgg
     3961 cggctgcgct cccatgccga ggaagccaag ggcggccata tcgagaatgg cgttagagaa
     4021 accgagcgac gcctgaacaa tcagcggcgc caggcagttc gggaagatgt taataaacat
     4081 ctgacgcatc gccccggcac ctgccacgcg agacgcggta acgtaatcgc gattcacctc
     4141 caccagcacg gcggcgcggg ttaagcgcac atagtgcggc aaggcaacga aggtcagtgc
     4201 cagcgcggcg ttaccaatcg acgggccgaa aatcgccacc agcaccagtg ccaacagcag
     4261 acttggcagc gccagcatga tatcgaccac gcgcatgatg atgttatcga ccaggccgcc
     4321 gaagtaaccg gcgatcaggc cgagaataac gcccataatc agcgataaca ccaccaccag
     4381 acagccaacc agcagcgaca agcgcgcgcc gtacatcagc cgcgacagca catcacggcc
     4441 tacgtcatcg gtgcccagca agtgcgccat gctgccgcct tcctgccagg ctggcggggc
     4501 gagcagcgca tcgcggaact gatccgccgg gttataaggc gcaatccagt tggcaaagat
     4561 cgtgataaac agcacgatga cgatgtaaac cagcccgaca accgcgcctt tgttgcgttt
     4621 aaaatagtgc cagaactcct gtaacggggt catcggcacc ggtgcgctaa tcactttatt
     4681 ttcagtaacc tgtgacatga tgtcccctta cttcttatga cgaatacgcg ggttcaccac
     4741 gccgtacagc agatcgacca gcaggttgac gaggataatc atcgtcgcca ccagcaatac
     4801 gccgccctgc actaccggat agtcgcggcg ttgcagtgcg tcaatcaacc agcgtcccag
     4861 accgggccac gagaagatgg tttcggtcag aatcgccccc gccagcaatg ttcccacctg
     4921 caggccgata acggtcacta ccggcagcat cgcgttacgc agcgcatgga cgataatcac
     4981 ccgcatgcgg gtcaaccctt tggcgcgcgc ggtgcggatg taatcttcac ccagcacttc
     5041 cagcatcgaa gagcgcgtca tacgaacgat aaccgccagc gggatagtac ccagcacaat
     5101 agccggtaag atcatatggg caacggcgtc gataaagttg ccatcttcac cccagatggc
     5161 ggtgtcgatc agcataaaac cggttaacgg attggagtca tcgaggaaca ccatatcgct
     5221 cacgcgaccg gagacgggcg tcaggttcca gtgcaccgaa accagcatga tcagcatcat
     5281 gccccaccag aagatcggca tcgagtagcc ggtcagcgcc aggccaactg cagtgtgatc
     5341 gaaaatggaa ccgcgtttaa ccgcagccag cacaccaacc ggaataccga ctgccgtagc
     5401 aaaaatcatc gcgcagacgc caagttccag cgtggcctga aagcgcggca cgaactcttc
     5461 ccatactgga attcggcttt tcattgaaat gcctagatcg ccgtgcatta cgccccaaat
     5521 gtaatggaga tactgctgcc acatcggttt atctaagccg agttcagcca gcagctgcgc
     5581 gtgacgctca ggggagatcc cgcgttcgcc tgccatgatc atcaccggat cgcccgggat
     5641 catgtggaca aaggcaaatg tgagaagggt aataccgata aacgtgggga tgacgagtcc
     5701 caaacgtcgg agaataaact gcaacataac ccggattctc tatagtgacc cgcagcctgg
     5761 aactgcgtgt ctgtattgct cacaaatcaa ttcccgccct tatcggacag ggaatgaagt
     5821 attgctgccg gatggcgctg cacgcagagg cacatcgcca ccgggtatac agattgttga
     5881 caaagtgcgc tttgtttatg ccggatgcgg cgttaacgcc ttatccggcc tacaaaatcg
     5941 tgcaaattca atacattgca gaattttcgt aggcctgata agcggagcac atcaggcagt
     6001 tttgcctttg ccatcagtct tgtatggctt ttaattattc gatagagacg ttttcgaagt
     6061 gatgtttgcc taatggatca accacatagc ctttgacttc tttgcgtacc ggttcaaaca
     6121 cggtggagtg agcgatgatc agtgccggag tctgatcgtg catcaccacc tgcgcttgct
     6181 tgtacagttc aacgcgtttg ttatggtcgt cggtagcacg cgccggttga atcagatctt
     6241 caaacggttt gtagcaccat tttgagtagt tagaaccttg ttcagaggcg gcgcagctga
     6301 acagggtggc gaagaagtta tccgggtccc cgttatcgcc agtccagccc atcattaccg
     6361 tctgatgttc gccatctttc gcacgcttga ggtactcacc ccattcgtag gtgacgattt
     6421 tggcctgcac gcccactttc gcccagtctg cctgaatcat ctccgccatg cggcgagcgt
     6481 tcgggttata cggacgttgt accggcatcg cccacaggtc gatggagaaa cctttttcca
     6541 gacccgcttc tttcagcaag gctttcgctt tttcaggatc gtaggtgtaa tcctgaacgt
     6601 cgtcgttata gccccacatg gttggcggga tcaggttttt cgctgataca cccgcgccct
     6661 gataaaccgc tttgatgatc gcgtctttgt tcaccgcgta ggtcagtgcc tggcgaactt
     6721 tcacgtcatc cagcggtttt ttctgcacgt tataggagag gtagccgacg ttcagccccg
     6781 gcatttccat caggttgatg gatttatcct gcttcatgcg agcgatgtct gccgggttcg
     6841 ggtacggcat cacctggcat tcattcttct gcaatttcgc gtaacgcacg gaagcatcag
     6901 gggtaataga gaaaaccagc gtatcgatct gcggtttggt gccccagtag ccatcaaacg
     6961 ctttgtagcg gatacgggaa tctttttggt actgctgtaa ctggaacgga ccggttccga
     7021 ttgggttgag gtccagtttt tccggtgtac cggctttcat catcgcatca gcatattctt
     7081 ttgacagaat agaggcgaag tccattgcca gatcagcgag gaacggcgct tccgggcgag
     7141 tcagtacgaa ctgaactgtg ttgtcgtcca cctttttcac ttcactgatc agctccggca
     7201 agcccatgcc ttcgaagtat tcgtagctgc cgccagagac tttatggtac gggttttgcg
     7261 cgtttttctg acggtcgaac gagaacacca cgtcatcggc gttcagttca cgcgtcggtt
     7321 tgaattcttt attgtcgtgc cacttcacac ctttacgcag atggaaggta taggttttac
     7381 cgtcttcgct gacttcccac ttttcagcga ggcccgggat cacttcagtg gtgccgattt
     7441 taaattcaac caggcggtta taaagcggta cggaagaggc gtcataggtg gtgccggagg
     7501 taaacagctg cgggttaaac ccttccggag atccttctga gcaataaacc agagttttag
     7561 cctgaacact tgctgcgacg gtcatagcca ccaggctgag accaagcttc agcatccctg
     7621 actttttcaa ggaaatacgc attattctgc tccaattgtg atgtttgttg ttttaaccct
     7681 ttgcagtggg ttgtcgctgc ctgacctttt ttgttttttg cccggtcggg tcaacgttat
     7741 gaggtgggga tgccgtacta attaacatca gtgtaacagt gcggatcctc cataaaatgc
     7801 ccctcgtgac ctacaatctg tcaacagaat gtgaaaacgt caatacagcc aaccgggatt
     7861 tacaccaacg gtgagaatcc acaaacaaag attaaaaaaa cttcaaacag ctatttgcag
     7921 cagagaaatt tgtgctactc caacataacc agaacaatca gcttaatatt tagcaacatt
     7981 tggtgattaa gttttatgca tttaatgaaa aaaatctgag gaaaaggtgg atatctgagc
     8041 gattaatacc atgaacgcta aaacgcacag cggaaaatgc cagcgccagc cataagtaac
     8101 gcaaaaaaag atttaagcaa atataaaaaa agacaatggt ttctgtcaca aatccgttaa
     8161 tggatagtga gatatggggc gcaaagttgg caggaaaatg tgtggtgtgt tggtcgaata
     8221 aggcggtcgc gccgcatccg acaataaatg cggtgccgtt gcctgatgcg acgctggcgc
     8281 gtcttatcag gcctac
//
LOCUS       DAEEKQ010000167         8272 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.169, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000167 DAEEKQ010000000
VERSION     DAEEKQ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8272
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.169"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            52..936
                     /locus_tag="KLK57_003500"
     CDS             52..936
                     /locus_tag="KLK57_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HBE0893777.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHNEDADGVALTFELTQSEETKKFCPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSISMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="KLK57_003501"
     CDS             complement(987..1841)
                     /gene="yeaE"
                     /locus_tag="KLK57_003501"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
                     /protein_id="HBE0893778.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLRDNVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHRSGSFAFEETVAAMEKLIAQGKIRRWGVSNLDYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHA
                     HNISSAQVLLAWVISHQGVMAIPKAATIAHVQQNAAVLEVELSSAELTMLDKAYPAPK
                     GKTALDMV"
     gene            complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="KLK57_003502"
     CDS             complement(1931..2677)
                     /gene="mipA"
                     /locus_tag="KLK57_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HBE0893779.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLTGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            3113..5047
                     /gene="yeaG"
                     /locus_tag="KLK57_003503"
     CDS             3113..5047
                     /gene="yeaG"
                     /locus_tag="KLK57_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HBE0893780.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMKGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            5160..6443
                     /locus_tag="KLK57_003504"
     CDS             5160..6443
                     /locus_tag="KLK57_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HBE0893781.1"
                     /translation="MTGFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
     gene            6590..8065
                     /locus_tag="KLK57_003505"
     CDS             6590..8065
                     /locus_tag="KLK57_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBE0893782.1"
                     /translation="MIQSTRISMGLFFKYFLSLTKIDPGQNYISLPSIKSSTHIALLF
                     MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQMNYLMVVVALFFLNA
                     VIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPINGSTLIQTKSNDVS
                     IFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPFLVFPLLAHNLSSYN
                     ADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRLASELWPLIALLCLA
                     SLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQKLQLSSKLAVHDVLT
                     NIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHRVGDKVLVSIVDIIQ
                     QSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLTGFSNRYDVPEQMTI
                     SIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
BASE COUNT         2192 a         1980 c         1914 g         2186 t
ORIGIN      
        1 cttcggtcgc tctttttttt acctgataaa atgaagttaa aggactgcgt catgattaag
       61 aaaatttttg cccttccggt catcgaacaa atctcccctg tcctctcccg tcgtaaactg
      121 gatgaactgg acctcattgt ggtcgatcat ccccaagtaa aagcctcttt tgcattacag
      181 ggcgcacacc ttctctcgtg gaaacctgcg ggtgaagaag aagttctgtg gttgagcaac
      241 aacacaccgt tcaaaaatgg cgtcgctatt cgcggtggcg taccggtttg ctggccgtgg
      301 tttggtccgg cggcacaaca aggtctgcct gcgcacggtt ttgcccgcaa cctgccgtgg
      361 acgctgaaat cacataatga agatgctgat ggcgtagcgc tgacttttga attgacgcaa
      421 agcgaagaga cgaaaaaatt ctgtccgcac gactttacgc tgttagcgca tttccgcgtg
      481 ggtaaaacct gtgaaatcga tcttgaatca catggcgaat ttgaaaccac ctctgccctg
      541 catacctact ttaacgtggg tgatatcgct aaggtaagcg tcagtgggct gggcgatcgc
      601 ttcattgata aagtgaatga cgcgaaagaa gatgtactga ccgacggtat tcagaccttc
      661 cctgaccgta ccgatcgcgt gtatctgaat ccgcaagatt gcagcgtgat taatgatgaa
      721 gcgctgaatc gtattatcgc cgtaggccac cagcatcatc tgaacgttgt aggctggaac
      781 ccgggaccgg cgctttcaat tagcatgggc gatatgccgg atgatggcta caaaacattt
      841 gtttgtgtag aaacggctta cgcttcagaa acgcaaaaag tcaccaaaga gaaacctgca
      901 catctggcgc aatccattcg cgttgcgaaa cgttaatttg cgttaatgtt gtgtgccggg
      961 tgcgatgcat ccggcacgta tcaacatcac accatatcca gcgcagtttt tccttttggt
     1021 gccggatacg ctttatccag catggttaat tccgctgacg aaagttcgac ctcaagcaca
     1081 gccgcattct gttgaacatg ggcaatcgtg gccgcttttg gaatcgccat cacaccctga
     1141 tggctgatta cccacgccaa caatacttgt gccgagctga tattgtgagc atgtgcaatt
     1201 tcgttgacta ccgcgttttt taacagtcca ttgcgcaacc gcccggcctg ggctaatgga
     1261 ctgtaagcca tcaccggcat ctgctgttgc tggcaccagg ggagtagatc gtactcaatc
     1321 cctcgtgaac cgagatggta aagcacctga ttagtggcac actgatttcc ccccggcagc
     1381 tgccagagtt cctgcatatc agcataatca aggttagaaa cgccccagcg gcggattttt
     1441 ccctgggcga tcaatttttc catcgctgca acagtctctt caaaagcgaa actgccagac
     1501 ctgtgtaata agtagagatc gagataatca gtattgagac ggcgtaaact ggcttcgcat
     1561 gcatttatcg ctttttgccc gccagcattc cacggataga ctttagagac gagaaagacg
     1621 ttatcacgca gaccggttaa tgcttcccca accacctttt cggcaccgcc atcggcatac
     1681 atttcggcgg tatcaatgag ggttaaaccg agctcaatgc ccgcgcgtag tgcagcaact
     1741 tctgttttgc gctgactggc atcttcgccc atataccatg ttccctgccc tatggctggc
     1801 agtgagacat cgccactaaa ttgaatcatt ttttgttgca ttgtttcctc ccaggtattg
     1861 caccaccgta atgcaaaaca gggcgtgacg ccctgttttt atgcacaaaa tgccctggaa
     1921 agatgcatta tcagaatttg taggtgatcc cggtagaaat caggccagtc caggatttat
     1981 ccaccatcgg gctgtcagta acttcatcag acagacgggt atagcgcgcg gtaccgtaaa
     2041 cactccagtc gccgaggaag ttgtagctgg cgctcagctc caggtaaggg ctccagctgt
     2101 cgttcgggtt atagccacgc agaccgctgc gagcggactc tttgcgcgat acgccataat
     2161 agtattcgtt ctggttttcg ctgttccact gcacaccgat acccggggtc actgtcaggc
     2221 cgccgttggt gtaacgatac aaccaggcca tatcccagac aatgccgttg ctgttatcca
     2281 gggtatcgcc agtcagggtg gtacgcaggt aaccgtactg ggtaaagtga gcataagaca
     2341 gaccagccat catggtgctc ttacggtcat ccaggtgacg catttggtga tcgccactgt
     2401 ctttcgcttt gaagtaaagc ggcgaccaat aagcggtaat tgaaagtttg tccgttgcgt
     2461 cattccacag gtagtaacca ccacctaagc cacggaacca gaagttatcg ccttcatagt
     2521 tgattaccgg tactgggtaa acatcggtat cgtaatcttt atatgggtgt tcaacgacac
     2581 cgacgcctgc gcccagggaa aatttacctt cagcgtgcgc tacgcctgca gacgttgcga
     2641 taagcactcc aagtgccaga agtttgagtt tggtcacaat taatcattcc ttaaacaaat
     2701 gtttagcggg cgacaaagtt tacccgtcaa tacatcgaaa cccaaccttt ttacgttttc
     2761 attttttaaa gtaactgttt aattttcctg acgcggatga caccgcgctt acagccaaat
     2821 gaatttagcg ttactggcga gccttgtctt tacattaatt atgcaaaatt tatggatgag
     2881 ttgttgatat gccattgaaa ttaagaaagc cgtgcaggca agttttccat ttgccatcta
     2941 cgcttaattt tgaaggtgta tcaccgggca cgttgttctc atcgtcgata aaatggcatg
     3001 agagttgctg tgttttagca agagacgtcg ttcagtttac ctcttccggg agcctctact
     3061 attcatatga acggctctta acctgtgcta aaaaacgaaa ggacggcata ccatgaatat
     3121 attcgatcac tatcgccagc gatatgaagc tgccaaggac gaagagttca cactgcagga
     3181 gtttcttacc acttgtcggc aagatcgcag tgcttatgcc aacgcggctg agcggctatt
     3241 gatggctatc ggtgagcctg tcatggtcga tacagcccag gaacccagac tttctcgact
     3301 cttttctaac cgggtcattg cacgttatcc ggcatttgaa gagttttacg gcatggaaga
     3361 cgcgattgaa cagattgtct cttatctgaa acacgcggct caggggctgg aagagaagaa
     3421 acaaatcctg tatctgctgg ggcctgtggg tgggggtaaa tcatcgcttg ctgagcgact
     3481 gaaatcatta atgcagctcg taccgattta tgtattgagc gccaacggtg agcgtagccc
     3541 ggtcaacgat catccgttct gtcttttcaa tccgcaggaa gatgcgcaga ttctggaaaa
     3601 agagtatggc attcctcgcc gctatctcgg caccatcatg tcgccgtggg cggcaaaacg
     3661 cctgcatgaa tttggtggcg atatcactaa gttccgggta gtgaaggtct ggccgtcaat
     3721 tctgcaacaa attgctatcg ccaaaacaga acccggtgat gagaacaacc aggacatctc
     3781 cgcgctggta gggaaagtcg atattcgtaa actcgaacac tacgcgcaga atgacccgga
     3841 cgcctacggc tattccggtg cgctgtgccg cgccaatcag gggatcatgg aatttgtcga
     3901 gatgtttaaa gcaccgatta aagtgctgca tccgctgtta accgctactc aggaaggtaa
     3961 ctacaacggg acggaaggta tctccgccct gccgttcaac gggattattc tcgcccactc
     4021 gaacgaatcc gaatgggtca ctttccgtaa taacaaaaac aacgaagcct tcctcgaccg
     4081 tgtttacatc gtgaaggtgc cgtattgctt acgcatttcc gaagagatca aaatctacga
     4141 gaaattgctt aatcacagtg aattgactca cgccccatgc gcccctggca cgctggaaac
     4201 actgtcacgt ttttccattc tttcgcgcct gaaagagcca gaaaactcca gcatttattc
     4261 aaagatgcgg gtttatgatg gcgaaagtct gaaagacacc gatcccaaag ccaagtcgta
     4321 tcaggaatat cgtgactacg ccggtgtcga tgaagggatg aaaggtctgt cgacgcgttt
     4381 tgcgtttaag atcctctccc gcgtgttcaa cttcgatcat gtagaagtgg cggcaaaccc
     4441 ggtccatctg ttctacgtcc tagaacagca gatcgagcgc gagcagttcc cgcaagagca
     4501 ggcagaacgc tatctggagt tcctgaaagg ttatctgatc ccgaaatatg ccgagtttat
     4561 cggcaaagag atccagacgg cctaccttga atcctattcc gaatatgggc aaaacatttt
     4621 cgaccgttat gttacctacg cggatttctg gattcaggat caggagtatc gcgatccgga
     4681 taccgggcag ctgtttgacc gcgagtctct taacgccgag ctggagaaaa tcgagaaacc
     4741 ggcggggatc agtaatccaa aagatttccg caacgagatt gttaacttcg tactgcgcgc
     4801 cagagcgaat aacagcggac gcaatccgaa ctggaccagc tatgaaaaac tgcgcacggt
     4861 tatcgagaag aaaatgttct ccaataccga ggagctgttg ccggttatct cgtttaacgc
     4921 caaaacgtca accgacgagc agaagaaaca cgacgacttt gtcgaccgta tgatggaaaa
     4981 aggctacacc cgtaaacagg tgcgtttact gtgcgaatgg tatttgcgcg tacgtaaatc
     5041 gtcttaacaa ccctggcccg gtacacatgc taccgggcct ataacgacag cgaaccgtgg
     5101 gccagagaaa tggcaacaca ggcgtagcat acagttggca aatgtagtac ggggggcata
     5161 tgaccgggtt tattgaccgg cgtctgaacg gcaaaaacaa aagcatggtg aatcgccagc
     5221 gttttttacg ccgttataaa gcgcaaatta aacagtcgat ctccgaggcc attaataagc
     5281 gttcggtgac tgacgtcgac agcggcgaat ccgtatccat tcccacggaa gatattagcg
     5341 aaccgatgtt tcatcagggg cgtggcggcc tgcgccaccg cgtacatccg ggcaatgacc
     5401 atttcgtcca gaacgaccga attgaacgtc cccagggtgg cggcggaggt tccggcagtg
     5461 gtcagggcca ggccagccag gatggtgaag gtcaggatga atttgtcttt cagatttcga
     5521 aagatgagta tcttgatctg ctctttgaag atttggcctt accgaatctg aaacaaaacc
     5581 aacaacgcca gctgaccgaa tataaaacgc atcgggcggg ttataccgct aacggcgttc
     5641 cagccaatat cagcgttgtg cgttccttgc agaactcact ggcgcgacgc acagccatga
     5701 cggcgggcaa gcgacgggaa cttcatgcgc tggaagagaa tttggccatc atcagcaaca
     5761 gtgaacctgc gcaactgctg gaagaggaac gtctgcgcaa agaaattgca gaattacgtg
     5821 ccaaaattga acgcgtccct tttattgaca ccttcgattt acgttacaag aactacgaga
     5881 agcggcccga tccctccagc caggcagtga tgttttgcct gatggacgtt tccggttcaa
     5941 tggatcaatc cactaaagat atggctaagc gtttttatat tctgctgtat ctgttcctca
     6001 gcagaacgta taagaacgtg gaagtcgtat acatccgcca tcatacccag gcgaaagaag
     6061 tcgatgaaca tgagtttttc tactcgcagg aaacaggcgg caccattgtt tccagcgccc
     6121 tgaaactgat ggatgaggta gtgaaagagc gttataaccc ggcacagtgg aatatttacg
     6181 ctgcacaagc atcggacggc gataactggg ccgatgactc tccgctttgc catgaaatcc
     6241 tggcgaaaaa attattacct gttgttcgtt attacagcta tatcgaaatt acccgtcgtg
     6301 cacatcagac attgtggcga gaatatgagc atctgcaatc tactttcgac aactttgcga
     6361 tgcagcacat ccgcgaccag gatgatattt atccggtgtt ccgtgaactg tttcataaac
     6421 aaaatgcaac agctaaagac taaaactatc agccaggtca ttatcgcctg gctgattttt
     6481 agcttactgt aaattatctc atttattaca tacattagct tacaatcgct ttaaatatga
     6541 cagcataacc tttacataat ttagttccag aaaacaatca ttcggaaaaa tgattcagtc
     6601 aacacgtatt tccatggggt tattctttaa atatttttta tcgttaacga aaattgatcc
     6661 tggtcaaaac tatatatctc tgccatcaat aaaatccagc actcacattg ctctcctttt
     6721 tatggtttct atgggtacac aaaaattaaa agctcaaagc ttttttattt tcagtttatt
     6781 gctgacgtta attttatttt gcattactac cttatataac gaaaacacaa atgtaaaact
     6841 catcccacag atgaattacc tgatggttgt tgtggctttg tttttcctta acgccgtcat
     6901 ttttcttttc atgttaatga aatatttcac taacaaacaa attttaccaa cactcatttt
     6961 aagccttgca tttttaagtg gccttatcta tttagttgaa accattgtaa ttatccataa
     7021 accaattaac ggcagtacac tgatccagac aaagtcgaat gatgtttcta ttttctatat
     7081 tttccgccaa ctcagtttta tttgtttaac ctcgctggca ctcttttgtt atggaaaaga
     7141 caacatcctt gacaacaata agaaaaaaac gggaatcctg ttgctggcgc tgattccttt
     7201 tttagttttt ccccttctgg cacacaatct gagcagttat aacgctgact attctttgta
     7261 tgtcgtcgac tactgtccgg acaaccatac tgcgacctgg ggaatcaact atacaaaaat
     7321 attggtttgt ctgtgggcat ttttactgtt ctttattatc atgcgtacac gattagccag
     7381 cgaactatgg ccgttaatag cattattatg tctggcatcg ctatgctgca acttacttct
     7441 actgactctg gatgagtata attacaccat ctggtatatc agtcgcggga ttgaagtttc
     7501 cagtaaactg tttgttgtgt cttttctgat ttataacatt tttcagaaac tgcaactctc
     7561 cagcaaactg gcagttcatg atgtgctgac caatatttat aatcggcgct actttttcaa
     7621 cagcgtagag tcattattgt cgagacctgt tgttaaggac ttctgtgtca tgctggttga
     7681 tattaatcag ttcaaacgca tcaatgccca atggggacat cgtgtgggtg ataaagtgct
     7741 ggtttcaatt gtcgatatta tccagcaaag catccgcccc gatgatattt tagcgcgact
     7801 ggagggtgag gtgtttggct tgctatttac cgaactcaat agtgcccagg caaaaatcat
     7861 tgcggaacgt atgcgtaaaa atgtcgaact cctgaccggc tttagtaaca gatatgatgt
     7921 tcctgaacaa atgaccatca gtattggcac ggttttttca acgggtgaca cgcgtaatat
     7981 ctcgcttgtc atgacggaag cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa
     8041 caaagtgatt attcatcata tttaagtgca aaaatattca gagccatgct ttttgcgtgg
     8101 cttttgcata caatttatta ctattacccc taaatttccc ctccccagcg gcgcagagat
     8161 gagtataatt agcgcccctg tgccaggccg caatcgaact ttatctggtt ttctcgtttc
     8221 actaaccgaa ggagtgccat ttatcatgaa attgcaccat agaatgctcc gg
//
LOCUS       DAEEKQ010000168         8255 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.170, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000168 DAEEKQ010000000
VERSION     DAEEKQ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8255
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.170"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(405..1601)
                     /gene="hemY"
                     /locus_tag="KLK57_003506"
     CDS             complement(405..1601)
                     /gene="hemY"
                     /locus_tag="KLK57_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HBE0893783.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1604..2839)
                     /gene="hemX"
                     /locus_tag="KLK57_003507"
     CDS             complement(1604..2839)
                     /gene="hemX"
                     /locus_tag="KLK57_003507"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBE0893784.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAAAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQATQLE
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPAPQGDTPAAAPQGE"
     gene            complement(2861..3601)
                     /gene="hemD"
                     /locus_tag="KLK57_003508"
     CDS             complement(2861..3601)
                     /gene="hemD"
                     /locus_tag="KLK57_003508"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HBE0893785.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGQQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPNYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTTVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNAANDALLRALQ"
     gene            complement(3598..4539)
                     /gene="hemC"
                     /locus_tag="KLK57_003509"
     CDS             complement(3598..4539)
                     /gene="hemC"
                     /locus_tag="KLK57_003509"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HBE0893786.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAKQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4926..7472
                     /gene="cyaA"
                     /locus_tag="KLK57_003510"
     CDS             4926..7472
                     /gene="cyaA"
                     /locus_tag="KLK57_003510"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HBE0893787.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7512..7832)
                     /gene="cyaY"
                     /locus_tag="KLK57_003511"
     CDS             complement(7512..7832)
                     /gene="cyaY"
                     /locus_tag="KLK57_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HBE0893788.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
BASE COUNT         1841 a         2175 c         2150 g         2089 t
ORIGIN      
        1 ttaattgaat gaattttatc attcataata agtatgtgta ggatcaagct caggttaaat
       61 attcactcag gaagcaaaga ggattacaga attatctcat aacaagtgtt aagggatgtt
      121 atttcccgat tctctgtggc ataataaacg ggtagatgct cattccatct cttatgttcg
      181 ccttagtgcc tcataaactc cggaatgacg cagagccgtt tacggtgctt atcgtccact
      241 gacagatgtc gcttatgcct catcagacac catggacaca acgttgagtg aagcacccac
      301 tgttgtcata cagacctgtt ttaacgcctg ctccgtaata agagcaggcg tttttttatg
      361 catcagaaag gtcccggagg tgcttgcctc cgggtgagaa ggaactactg tggtgggttg
      421 ttctgcaacg ttaacatcaa accgtcgcga cgcatcgctg cagcttcttc cggcttgtgc
      481 agtctgtcca gcgcgtcggc aagccatgcg taatcgtagg cgtccggacg ttgtttcagc
      541 gctgcgcgga aggcgagcga tgcttcctgc cattctccgt gcttcatcag tgactggccc
      601 agtgtgctcc acaacagcgg gcgatcgccg acgtttttga tctgctggcg cagcactttt
      661 tccagctgtt ccggattgtt ggttttcagt cgaggaatcg gcagcagtag gcgatcgtcg
      721 tactggcgtt tcaggccatc gatgataatt tgctgggcag tatcatgatc gtcacattca
      781 ataagatgtt ccgccattgc cacctgcaac gctacctgat gacgcgtttt ccggctttgg
      841 tttttccacc agttacgcaa accttcacta ccgttatcgg cacgcgcctg atccatcagg
      901 ccaatccatg cctgttgttc cagcattgca cgatgttctt catcaccaac atgggctttc
      961 gccattgatg ggatgatatc cagcagagaa ctccatgcac ctgtgcggat atacgcctgt
     1021 tccgccagac gtaatacttc cggatggcgt ggcgtaactt ccagcagctt atccacgccg
     1081 tggcgtgcag catggttttc attacgggcc agttgcagac gtacacgggt gatttctacc
     1141 ggaatggtgt cattgccggc cagctctgct gcgcgttcca gatgttggtt ggcgcgtgct
     1201 tcatcaccac gttgttgcgc ggcttcggca gccagtagat agttcaccac cggttgttcc
     1261 gcgtgatcgg catttttcgc catcagcttt tcaacttgct gataatcgcc ttccgccagt
     1321 ttcagcagcg cctgttcggt ctgcttacgg gcacggcgac gcttacgtcc gacaaaccac
     1381 ccacgggtgt gtgcgcctgt gcggaagatc cgccgcagta gccactcaat ggcaaacagc
     1441 actaccatcg ccagaatcaa aataatcgcc aggcccgtga cgctggtttc gatattgtag
     1501 ttgtcagtct ggatcagcac gtaaccctga tgaccggcaa tcatcgggcc aaccactatc
     1561 cccgcaatca gcaaaacaaa gagtaataac acttttagca tggttattct ccttgcggcg
     1621 cggctgccgg agtatcacct tgcggtgcag gtgcaggtgc aggtgcaggt gcaggtgcag
     1681 gtgcaggtgc aggtgcaggt gcaggtgcag gtttagcttc cgttgtcccc gctgccggtt
     1741 gtgccagcag gttacgcacg cgggtctgca tcagtttttc cagcatcgcc tggctttgca
     1801 gggtttccgg aagatccatc gagatatttt gctggcttaa ctggtccacc tcgtcgagga
     1861 acgctttggt ggtggcatca tcagtatcgt agtaagcacg tacccaggtg gagacgttct
     1921 ccagcgcctg gcgataagtc tcttcctgat gacgcggtac agcttgtgct gcgaccagca
     1981 ggcgagagcg aatattttcg cgcagataga tatcctgatt tggcgctaac agcggtacgg
     2041 cggtgtcatc acgacggcga atcgtaatga agttgtccat aaagttctgc cagctttttt
     2101 gcagattgat acgccattcg ctgatggaac tggaaagctc ttcaccgtct gaatccatcg
     2161 gcgaaccatc gctgtcatta tcggccagac gcaggttatc tacctgattt gaaagctgat
     2221 taagcttaag gatgatgccg tcataatcca cctgcgatac tgcagaaagg ctggcgatat
     2281 catcggtaat tgcccgacga acggtaatca gactcgggtc attcatatcc gccaggctgg
     2341 cgtctgcact tttcagcaac gctgcagcgg tcgtgacgtc ctgatcgctc cacagcttcc
     2401 gtccggcgag tttcaccaga aaatcggcct gggccagcag ccaggtttta gcatcgctgc
     2461 cggaaatggt ggcgaccttt tgctggactt catccagctg ttttgccagc gtttcttgct
     2521 gacgattcgc ctgttcaagc tgcgtagcct gttgcttaat aatgccttcc agttcggctt
     2581 tttggctctc ctgggctttt tgcaatgccg tcagttggtt agccagggca tcgctggtgg
     2641 cggtctgatt gacggcctgt tgtttacccc agccatacaa accgacgccc gccgccagag
     2701 caatagcgat agccgccgcg ctgagaatca atgcggtatt gttcttactc tttttttctg
     2761 ttgcgacagg ttgtgacgtg gtgtccacgg cctccctagt ctcttcaacc acggcggagg
     2821 ttttttcttg ttccgtcatt atggcttcct gttatgagag ttattgtaat gcccgtaaaa
     2881 gcgcatcgtt ggcagcgtta tcggcgacct taatgtcttg ccagcccagt tcccgggcga
     2941 gtttcgccaa acgctcactg acgaccaata gtcgacagtg tagtaaccag tgctcacgat
     3001 accattgtgg gatcagcgac cagagttgct gcaacatttc accgctggta acaacgaccg
     3061 tcgtcacctc gcgggattgc cagcgcatcg cttcttctgc accatcgtaa tggattgcgc
     3121 atcgttgata acattcacaa aaagtgacct cagcaccgcg cgccgtcagg gtatccccaa
     3181 ttagctcacg accgccattg ccacgtaata tcagcgcacg tttgcccgca atattttgta
     3241 attcaggtaa ttgtagcaag acttcgctga tttcccgatc ctgcgggtag agaatcttct
     3301 gtccgcttac ggtatgtagt gccagtgcgg tggtgcgtcc aatggcgaaa taattaggta
     3361 gtcggggcca tttacgatct tgctgatgta gctgtgattg ggcaaaagca accgcgtgtt
     3421 gcgagagggc aaacaacaga tcgctctccc ccagcgctgc cagttgatca gcaagttgcg
     3481 gtaattgttg acccggagaa aactcaatca gcggaaaatg ccaggccacc tgccccagtg
     3541 tgcgcagacg gctcactaac tcttctccag cgggagacgg gcgggtgaca aggatactca
     3601 tgccggggcg tctccgttat agacttcagc gaggatctcg cgcgcgccgt tattcagtag
     3661 ctcttctgcc agcgaaatcc ccatttgttt ggcattttgc ggcgcaccgc ggcgttcacc
     3721 gcgaataatc tgcgaaccgt ccggcgcgcc gaccagcgca cgcagccaga tttcgccatc
     3781 aataagctcg gcgtagctac caattggcac ctgacatccg ccttcgagac gggtattcat
     3841 ggcgcgttct gcggtaacgc gcagtgcagt ttcgtggtga ttcagcgcgg caagcagctc
     3901 gcgagtgcgc gtatcatcaa ggcggcattc aataccgact gcaccttgtc ctaccgccgg
     3961 aagagaaatc tcgggtggta acgcggcgcg aatgcgggac tccagaccta aacgttttag
     4021 tccggctacg gcaagaatga tggcatcgta ttcgccgtta tctagtttgc tcaggcgagt
     4081 gccgacgttg ccgcgcaggg agcggataat cagatccggg cggcgttcag ccagttggca
     4141 ctggcgacgt aaactggacg tcccgacgat actgcctgct ggtaacgcat ccagactgtc
     4201 atagttattg gacacaaagg catcgcgagg atcttcacgc tcacaaatag tgaccagtcc
     4261 cagaccttgc gggaattcaa ccggcacatc tttcattgaa tgtacggcga tatcggcgcg
     4321 attttcgagg agcgcgactt ccagctcttt tacaaataag ccttttccgc ctactttcgc
     4381 cagcggcgta tcaagaatca catcgccgcg cgtcaccatc ggtaccagtt caacgaccag
     4441 gcccggatgg ctcgccatca acttgtcttt gacatagtgt gcctgccaga gtgcaagtgg
     4501 gctttggcgt gtggcaattc ttaaaacatt gtctaacatg cttgttaccg tcattatcat
     4561 ccgtggtcca tcctaacatc cttgccagag tgatgtcagt gttgtggtga aacgtagacg
     4621 cctgcgcaaa ccgtaaaatg aggtctggca gtggatcctg acagacgttt cacgctgtcg
     4681 taataaggaa tttacagaga ataaacggtg ctacacttgt atgtagcgca tctttcttta
     4741 cggtcaatca gcaaggtgtt aaattgatca cgttttagac cattttttcg tcgtgaaact
     4801 aaaaaaacca ggcgcgaaaa gtggtaacgg ttacctttga catacgaaat atcccgaatg
     4861 ccgcgtgtta ccgttgatgt tggcggaatc acagtcatga cgggtagcaa atcaggcgat
     4921 acgtcttgta cctctatatt gagactctga aacagagact ggatgccata aatcaattgc
     4981 gtgtggatcg cgcgcttgct gctatggggc ctgcattcca acaggtctac agtctactgc
     5041 cgacattgtt gcactatcac catccgctaa tgccgggtta ccttgatggt aacgttccca
     5101 aaggcatttg cctttacacg cctgatgaaa ctcaacgcca ctacctgaac gagcttgaac
     5161 tgtatcgtgg aatgtcagta caggatccac cgaaaggtga gcttccaatt actggtgtat
     5221 acaccatggg cagcacctcg tccgtagggc aaagttgttc ctctgacctg gatatctggg
     5281 tctgtcatca atcctggctc gatagcgaag agcgccagtt actacaacgt aaatgcagcc
     5341 tgctggaaag ctgggccgcc tcgctgggtg tggaagtcag cttcttcctg attgatgaaa
     5401 accgctttcg tcataatgaa agcggcagcc tggggggcga agattgtggc tccacccagc
     5461 atatactgct gcttgacgaa ttttatcgta ccgccgtgcg tctcgccggt aagcgtattc
     5521 tgtggaatat ggtgccgtgc gacgaagaag agcattacga cgactatgtg atgacgcttt
     5581 acgcgcaggg cgtgctgacg ccaaatgaat ggctggatct cggtggctta agctcgcttt
     5641 ctgctgaaga gtactttggt gccagccttt ggcagctcta caagagtatc gattccccat
     5701 acaaagcggt gctgaaaaca ctgctgctgg aagcctattc ctgggaatac ccgaacccac
     5761 gtctgctggc gaaagatatc aaacagcgtt tgcacgacgg cgagattgta tcgtttggcc
     5821 tcgatccata ctgcatgatg ctggagcgtg ttactgaata cctgacggcg attgaagatt
     5881 tcacccgtct ggatttagta cgtcgctgct tctatttaaa agtgtgcgaa aaactcagcc
     5941 gtgaacgcgc ctgtgtaggc tggcgtcgcg cagtgttgag ccagttagtg agcgagtggg
     6001 gttgggacga agctcgtctg gcaatgctcg ataaccgtgc taactggaag attgatcagg
     6061 tgcgtgaggc gcacaacgag ttgctcgacg cgatgatgca gagctaccgt aatctgatcc
     6121 gctttgcgcg tcgcaataac cttagcgtct ccgccagtcc gcaggatatc ggcgtgctga
     6181 cgcgtaagct gtatgccgcg tttgaagcat taccaggtaa agtgacgctg gtaaacccgc
     6241 agatttcacc cgatctctcg gaaccgaatc tgacctttat ttatgtgccg ccgggccggg
     6301 ctaaccgttc aggttggtat ctgtataacc gcgcgccaaa tattgagtcg atcatcagcc
     6361 atcagccgct ggaatataac cgttacctga ataaactggt ggcgtgggca tggtttaacg
     6421 gtctgctgac ctcgcgcacc cgtttgtata ttaaaggtaa cggcattgtc gatttgccta
     6481 agttgcagga gatggtcgcc gacgtgtcgc accatttccc gctgcgctta cctgcaccga
     6541 caccgaaggc gctctacagc ccgtgtgaga tccgccatct ggcgattatc gttaacctgg
     6601 aatatgaccc gacagcggcg ttccgcaatc aggtggtgca ttttgatttc cgtaagctgg
     6661 atgtcttcag ctttggcgag aatcaaaatt gcctggtagg tagcgttgac ctgctgtacc
     6721 gcaactcgtg gaacgaagtg cgtacgctgc acttcaacgg cgagcaatcg atgatcgaag
     6781 ccctgaaaac tattctcggc aaaatgcatc aggacgccgc accgccagat agcgtggaag
     6841 tcttctgtta tagccagcat ctgcgcgggt taatccgtac tcgcgtgcag caactggttt
     6901 ctgagtgtat tgaactgcgc ctttccagca cccgccagga aaccgggcgt ttcaaggcgc
     6961 tgcgcgtttc tggtcaaacc tggggcttgt tcttcgaacg cctgaatgta tcggtacaga
     7021 agctggagaa cgccatcgag ttttatggcg ctatttcgca taacaaactg cacggcctgt
     7081 cagtgcaggt tgaaaccaat cacgtcaaat taccggcggt ggtggacggc tttgctagtg
     7141 aagggatcat ccagttcttc ttcgaagaaa cgcaagacga gaatggcttt aatatctaca
     7201 ttcttgacga aagcaaccgg gttgaggtgt atcaccactg cgaaggcagc aaagaggaac
     7261 tggtgcgtga cgtcagtcgc ttctactcgt catcgcacga ccgcttcacc tacggctcaa
     7321 gcttcatcaa cttcaacctg ccgcagttct atcagattgt gaaggttgat ggtcgtgaac
     7381 aggtgattcc gttccgcaca aaatctatcg gtaacatgcc gcctgccaat caggatcacg
     7441 atacgccgct attacagcaa tatttttcgt gatgaacgtg ccggaaagcg aggcttatcc
     7501 ggcatgcaat cttagcggaa actgactgtt tcacccgcct gctgcgtcgc cgcctgttcc
     7561 agcaaatccc agaaggtttc gccgctgcga tcgcaaatcc actcatcgcc tttcagatca
     7621 aaatggtagc cgccttgttt ggttgccagc catacctggt gcagcggttc ctggcggttg
     7681 ataatgattt tgctgccgtt ttcaaaggta atggtcagca cgccgccgtt gatttcgcag
     7741 tcgatatcgc tgtcgccatc ccagtcgtcc aggcgttctt caatggtcag ccagagttga
     7801 tcagccaggc gatgaaattc actgtcgttc attgttgtat cctgttttta agtgatggcg
     7861 gcagtatagc ggcatggggt cagggcttca aagtttgcac ctctgcggct gcgttccggc
     7921 acgattcatc cgtcaccgga ataatgatgt ctctgtgtag cgaaagattt gtctcttcat
     7981 tagggcgcag ttacaccacg cctttccctg tttctggtaa acattatgat caggtttacc
     8041 gagcgagcat cctcacgctg acggaactaa aaaagacaac aaacaaacca cattgcgata
     8101 gtgcatagag ccatcctggc gcgaggtgcc gatcacgaaa ctaccagcaa aacataaatc
     8161 cccacgagta agcgttatac tcgcagcatt tcctcacttt tcagacttca taaagagtcg
     8221 ctaaacgctt gcttttacgt cttctcctgc gatga
//
LOCUS       DAEEKQ010000169         8212 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.171, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000169 DAEEKQ010000000
VERSION     DAEEKQ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8212
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.171"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="KLK57_003512"
     CDS             complement(215..1105)
                     /gene="dgcZ"
                     /locus_tag="KLK57_003512"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HBE0893789.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTDPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="KLK57_003513"
     CDS             complement(1360..1752)
                     /gene="ydeI"
                     /locus_tag="KLK57_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HBE0893790.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINVVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
     gene            2028..2546
                     /locus_tag="KLK57_003514"
     CDS             2028..2546
                     /locus_tag="KLK57_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HBE0893791.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="KLK57_003515"
     CDS             complement(2591..4636)
                     /gene="dcp"
                     /locus_tag="KLK57_003515"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HBE0893792.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            4773..5519
                     /gene="ydfG"
                     /locus_tag="KLK57_003516"
     CDS             4773..5519
                     /gene="ydfG"
                     /locus_tag="KLK57_003516"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HBE0893793.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5608..6294
                     /gene="ydfH"
                     /locus_tag="KLK57_003517"
     CDS             5608..6294
                     /gene="ydfH"
                     /locus_tag="KLK57_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HBE0893794.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            6471..6674
                     /gene="ydfZ"
                     /locus_tag="KLK57_003518"
     CDS             6471..6674
                     /gene="ydfZ"
                     /locus_tag="KLK57_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HBE0893795.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(6710..8170)
                     /locus_tag="KLK57_003519"
     CDS             complement(6710..8170)
                     /locus_tag="KLK57_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HBE0893796.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIIHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAKAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIERYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
BASE COUNT         2170 a         2055 c         1870 g         2117 t
ORIGIN      
        1 cggagataat ccctcaccta accggcccct tgttacagtt gtgtacaagg ggcctgattt
       61 ttatgacggc gaaaaaaaac cgccagtaaa ccggcggtga atgcttgcat ggatagattt
      121 gtgttttgct tttacgctaa caggcatttt cctgcactga taacggatcg ttgacacagt
      181 agcatcagtt ttctcaatga atgttaaacg gagcttaaac tcggttaatc acattttgtt
      241 cgtcaataaa catgcagcga tttcttccgg tttgcttacc ctcatacatt gcccggtctg
      301 ctcttccaat gaccacatcc agaggctctt caggaaatgc gcgactcaca cctgctgtca
      361 cggtaatgtt gatatgccct tcagaatgtg tgatggcatg gttatcgact aactggcaaa
      421 ttctgacacc tgcacgacat gcttcttcat cattagccgc tttaacaata atgataaatt
      481 cttcgccccc gtagcgataa accgtttcgt aatcacgcgt ccaactggct aagtaagttg
      541 ccagggtgcg taatactaca tcgccgatta aatgcccgta ggtatcatta accaatttaa
      601 atcggtcaat atccaacaac attaaataaa gattcagagg atcagtgttg cgtaactgat
      661 gatcaaagga ttcatcaaga acccgacgcc ccggcaatcc cgtcaaaaca tccatattgc
      721 tacggatcgt cagcaaataa attttgtaat cggttaatgc cgcagtaaaa gaaagcaacc
      781 cctcctgaaa ggcgtcgaaa tgcgcgtcct gccagtgatt ttcaacaata gccagcatta
      841 attcccgacc acagttatgc atatgttgat gggcagaatc cattagccga acgtaaggta
      901 attcatcgtt atcgagtggc cccagatgat caatccaccg accaaactgg cacagtccat
      961 aagaatggtt atccgttatt tctggcttac tggcatctct cgcgaccacg ctgtgaaaca
     1021 tactcaccag ccactggtag tgggcatcga tagccttatt gagatttaac aagatggcat
     1081 caatttccgt tgtcttcttg atcattgcca ctcctttttc acagttcctt gtgcgcgcta
     1141 ttctaacgag agaaaagcaa aattacgtca atattttcat agaaatccga agttatgagt
     1201 catctctgag ataacattgt gatttaaaac aaaatcagcg gataaaaaag tgtttaattc
     1261 tgtaaatcac ctctgcatta tcgtaaataa aaggatgaca aatagcataa cccaataccc
     1321 taatggccca gtagttcagg ccatcaggct aatttatttt tatttctgca aatgagtgac
     1381 ccgaacgacg gccggcgcgc ttttcttatc cagactgccg ctaatgttga tcatctggtc
     1441 cggctgaact tctcgtccat caaagacggc cgcaggaata acaacattaa tttcaccgct
     1501 cttatcgcga aaaacgtaac ggtcctctcc tttgtgagaa atcaaattac cgcgtagtga
     1561 aaccgaagcg ccatcgtgca tgttttttgc gaaatcaacg gtcatttttt ttgcatcatc
     1621 ggttccgcga tagccatctt ctattgcatg aggcggcggt ggcgctgcat cctgttttaa
     1681 accgccctgg tcatctgcca acgcataagg catgacaaga aaacttgcta atacaatgga
     1741 ctgaaatttc atactaactc cttaattgcg tttggtttga cttattaagt ctggttgcta
     1801 tttttataat tgccaaataa gaatactgcc aattgttata aggcatttaa aatcagccca
     1861 ctagctgtca aatattcaga gaatttgact cactaaagtt aagaagattg aagagtctta
     1921 aacatgtttt cagaataatc ggatttatat gtttgaaaat tattatattg gacgagcata
     1981 cagaaaaagc aaatcacctt tacatataat agcgtggaca aaaaacagtg aacattaata
     2041 aagataaaat tgtacaactc gcagataccg acactattga aaacctgaca tccgcgttga
     2101 gtcaaagact tatcgcggat caattacgct taactaccgc cgaatcatgc accggcggta
     2161 agttggctag cgccctgtgt gcagctgaag atacacccaa attttacggt gcaggctttg
     2221 ttactttcac cgatcaggca aagatgaaaa tcctcagcgt aagccagcag tctcttgaac
     2281 gatattctgc ggtgagtgag aaagtggcag cagaaatggc aacaggtgcc atagagcgtg
     2341 cggatgctga tgtcagtatt gccattaccg gctacggcgg accggagggc ggtgaagatg
     2401 gtacgccagc gggtaccgtc tggttcgcgt ggcatattaa aggccagaac tacactgcgg
     2461 ttatgcattt tgctggcgac tgcgaaacgg tattagcttt agcggtgagg tttgccctcg
     2521 cccagctgct gcaattactg ctataaccag gctggcctgg cgatatcccc aggccagcca
     2581 ttggtggtgt ttatatgttc aagccacgat gttgcagcat cggcataatc tgaggagcct
     2641 taccgcgcca ttgtcgatgc aggcgttcca gatcttcgct gttacctctg gaaaggatcg
     2701 cctcgcgaaa acgctgccca ttttcacgcg ttaatccgcc ctgctcaaca aaccactggt
     2761 aaccatcatc ggccaacatt tgcgtccaca gataagcgta ataacctgca gcatatccgc
     2821 caccaaaaat atgggcgaaa taactgctgc gatagcgtgg cggtatagca ggaagatcca
     2881 tattttccgc caccagcgcc cgcaattcaa aatcatcgac atcctgcatt gcttcgtttt
     2941 cttccaggca atgccagcgc atatcgagaa gtgcggcgct aagcagttcg ctcatctcat
     3001 accctttgtt gaacaggctg gcattacgca ttttctgttg cagttcgtca ggcattgctg
     3061 ccccgctctg ataatgccgg gcgtagcgag cgaatacctg cggatgcgtt gcccagtgtt
     3121 cgttgatttg cgacggaaat tcgacaaaat cacgcggcgt gttggtgccg gaaagcgtgg
     3181 cataacgctg gcgggcaaaa aggccgtgca gcgtatgacc aaattcatgg aataaggtta
     3241 ttacatcatc ccagagtaac aacgcaggct caccggcagc gggtttctga taattgcaga
     3301 cgttataaat taccggatgc gtttcattaa gcgttgattg ctcaacaaaa ttgcccatcc
     3361 atgcaccgcc gctttttgaa tcacgggcga agaaatcacc gtaaaataac gccagcccca
     3421 cgccattatg atcaaaaatt tcccacaccc gaacgtcagg atggtagaca ggaatatcaa
     3481 aacgttcgac aaacttaata ccgaagagct gattcgcggt ccagaataca ccttcattca
     3541 acaccgtgtt taattcaaaa tatggcttga gctgcgcttc atcaagatca aatttctccc
     3601 gccgtacctg ttccgcataa aatgcccagt cccacggctg cgcgctaaac ccgccctgct
     3661 gcttatcgat aaccgcctgt atggaggcta attcatcgct cgcacgttga cgcgccgctg
     3721 gaacaatttc ccgcataaag ttgagtgctg cttctggcgt ttttgccatc tgatcggcga
     3781 ttttccatgc ggcataatga ggaaaaccaa gcagtttcgc ctgctgtgcg cgaatctcta
     3841 ccagacgttg aatgatagcg cgggtatcat tgccatcatt tttttccgct cgcgtccagc
     3901 ccgcaataaa cagtttttca cgcgtcgcac gatcgcgcat ttcggcaagc gccggttgtt
     3961 gggtggtatt cagcagcgga atcagccatt tgttatccag acctttctcg cgagccgcct
     4021 ctgccgccag cgcaatctct tgctcactca ttcctgccag ctgcgcgata tcgttcacaa
     4081 ccagaccgcc ggatttattt gctgccagta atcgctggtt aaactggctg gtcagggtcg
     4141 cagcttctgt attcagtact tttaattttg ctttatcagc ttgcgcaagt ttggctccgg
     4201 caaggacaaa acgttgatga atcacctcca ccaggcggat ggattcacta tcaagcccca
     4261 gggattcacg gcgctgccag acagcatcta cccgcgcgaa taattcaccg ttcagataga
     4321 tatcattagc cagttccgcc agttcagcgg aaaactgttc atcaagacgc tgtaattcat
     4381 cattggtatg cgccgcagtc atcgcaaaaa agacgctggt aacgcgggta agtaattctc
     4441 cgctttgttc cagtgccaga atagtattgt tgaaatcagg cgtttgcggg ttaagcgcga
     4501 tggcagcaat ttctgcccgc ttttgctgca ttccctcatc gaatgccggg cgatagtgat
     4561 gattggcaat ttgatcaaaa tggggagcca gatacggtag tgtgctttgc acaaggaaag
     4621 gattcattgt tgtcattttc ttctcctgaa cgcgagatgt tccatagcgt aggcttactg
     4681 ataacggagt gcaatcttgc aaaccagtat tacccgctct taagcatcac gtgctatgtt
     4741 attgacacac aaaagcgttg aggaacagtg agatgatcgt tttagtaact ggagcaacgg
     4801 caggttttgg tgaatgcatt actcgtcgtt ttattcaaca agggcataaa gttatcgcca
     4861 ctggccgtcg ccaggagcgg ttgcaggagt taaaagacga actgggagat aatctgtata
     4921 tcgcccaact ggacgttcgc aaccgcgccg ctattgaaga gatgctggca tcgcttcctg
     4981 ccgagtggtg caatattgat atcctggtaa ataatgccgg cctggcgttg ggcatggagc
     5041 ctgcgcataa agccagcgtt gaagactggg aaacgatgat tgataccaac aacaaaggcc
     5101 tggtatatat gacgcgcgcc gtcttaccgg gtatggttga acgtaatcat ggtcatatta
     5161 ttaacattgg ctcaacggca ggtagctggc cgtatgccgg tggtaacgtt tacggtgcga
     5221 cgaaagcgtt tgttcgtcag tttagcctga atctgcgtac ggacctgcat ggtacggcgg
     5281 tgcgcgtcac cgacatcgaa ccgggtctgg tgggtggcac cgagttttcc aatgtccgct
     5341 ttaaaggcga tgacggtaaa gcggaaaaaa cctatcaaaa taccgttgca ttgacgccag
     5401 aagatgtcag cgaagccgtc tggtgggtgt caacgctgcc tgctcacgtc aatatcaata
     5461 ccctggaaat gatgccggtt acccaaagct atgccggact gaatgtccac cgtcagtaat
     5521 ttttataccc ggcgtaactg ccgggttatt gcttgtcaca aaaaagtggt agactcatgc
     5581 agttaactca ctcacaagca agaacgaatg accgtcgaaa cgcaacttaa tcccacacag
     5641 cctgtcaatc agcaaattta tcgtattctt cgtcgcgaca ttgtccattg cctgattgca
     5701 ccaggcacac cgttgtcgga aaaagaagtt tctgttcgtt tcaatgtgtc acgccagccg
     5761 gttcgtgaag cctttattaa actggcggaa aacggcctga ttcaaattcg tccgcagcgt
     5821 ggcagctacg tcaacaaaat ttccatggcc caggtgcgca atggcagttt tatccgtcag
     5881 gccattgagt gcgcggtggc gcgtcgggcg gcgagcatga ttaccgaaag ccagtgctac
     5941 caactggaac aaaatcttca ccagcaacgc attgccattg agcgcaagca actggatgat
     6001 ttttttgaac ttgatgacaa cttccatcaa ctcctgacgc agattgccga ctgtcaactg
     6061 gcgtgggata ccattgagaa cctgaaagcg accgttgatc gcgtgcgcta tatgagtttc
     6121 gaccacgttt ctccaccaga aatgctgtta cgccagcatc ttgatatttt ctctgccctg
     6181 caaaaacgtg atggcgatgc ggtagaacgt gcaatgacgc aacatttgca ggaaatcagc
     6241 gaatccgtgc gccagatccg ccaggaaaat agcgactggt ttagcgaaga gtaattcatt
     6301 tcctctcatc ccatccgggg tgagagtctt ttcccccgcc ttatggctca tgcatgtatc
     6361 aaaaaagatg tgagcttgat caaaaacaaa aaatatttca ctcgacagga gtatttatat
     6421 tgcgcccgtt acgtgggctt cgactgtaaa tcagaaagga gaaaatacct atgacgacct
     6481 acgatcgtaa ccgtaacgca atcaccactg gcagccgtgt tatggttagc ggcaccggtc
     6541 acactggcaa gatcctgtcg attgatactg aaggtctgac cgctgagcaa atccgccgcg
     6601 gaaaaaccgt agttgttgaa ggttgtgaag agaaactggc acctctggac ctgattcgtc
     6661 tcggcatgaa ctaagcgtgt gaatgccgcc gatggcggca ttgctttttt tacttcacgg
     6721 aatatttcgc cacggtcgct ttcgcgccat gcgctaataa agacaagtac gcttccgtca
     6781 cttttgcagt aaacaaacta ttgtctggca aatcatcacc aaagatcgcc ttaatcgcca
     6841 gcaatgactg gacgcgcgct ttcccttcag cactactttg tacagccttc tgaataacag
     6901 gtaacagtgg gtcactgatt tcaatcggat ttccctgttc atcaacacca ccgacataac
     6961 gcatccaacc cgcgacgccc agtgccagca gatcgaactt gctgtcatgc gccagatgcc
     7021 agcgaacaga atccaacatc cgctgtggca atttctggct gccatccatc gcaatctgcc
     7081 aggttcgatg acgtaacgcc gggttgctat agcgttcaat taatcggtta gcgtaatctt
     7141 gcaaatcaac gccctgcact ttcagcgtcg gcgcttgttc ctgcaacatc aagccatacg
     7201 ccgcataacg ataatgttca tcttccatgc agtcattaat gtgctgatat ccagcaagat
     7261 aacccaaata cgccaggaat gaatgactgc cgttgagcat gcgcaacttc atctcttcat
     7321 aaggcagcac atcactaacc agttcggctc ccgctttttc ccattctgga cgtccggcaa
     7381 caaagttatc ttctattacc cactggcgga aaggttcaca ggcaacgccc gcaggatcgc
     7441 ccacaccggt aagttgttcg attttcgcca gcgtatcctc tgtcactgcg ggcacaatac
     7501 ggtccaccat tgttgatggg aaagtcacgt tatcttcgat ccattgtgcc agttttacat
     7561 caacagcttt cgcgtatgaa gtgacaacgt cacgcataac atgaccgttt tctggcatgt
     7621 tgtcacatga catgacggta aatgcgggaa gtcctgccgc tttacggcgg gccagcgcct
     7681 caacaatcac ccctgttgct gttttcggct ggtggggatt ttgcacgtcg gcagcgacca
     7741 tcgggtgatc gagcattaac tgtccggtcg ccggagagtg gaaataccct ttttcggtga
     7801 ttgtcagaga gacaatcgcg atttgcggtt cacacatcgc agccaacacg gtttctaagc
     7861 catccatctg tacgtgcaag gctttcttaa cgacgccaac gacgcgagcc gtccacgcat
     7921 cggccgacat ttccgcaacg gtataaagat tatcttgctg ttgtaaatcg gcaatttgct
     7981 gttcgccgcc gattaagttg acctcataat atccccagtc actgaaatgt tctgtagcaa
     8041 gaatatcggc atacacgccc tgatgcgcac ggtgaaatgc accaaagcct aaatgaataa
     8101 ttcttggggc caggttatta cgatcataaa cagggagtgt cgcttttgct gataacaaat
     8161 tatttcccat aacaattcct taaatataaa tatggcaaac tatatgtttt gt
//
LOCUS       DAEEKQ010000170         8183 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.172, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000170 DAEEKQ010000000
VERSION     DAEEKQ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.172"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..2848
                     /gene="yfaL"
                     /locus_tag="KLK57_003520"
     CDS             <1..2848
                     /gene="yfaL"
                     /locus_tag="KLK57_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HBE0893797.1"
                     /translation="ADGKTLVIGNTENDGAVDSIAGTGLITKTGSGDLVLNADNNDFT
                     GEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQNQAELNVGSTQQT
                     FVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSYVLSGAQSMALTG
                     DIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQSGTSYNDGLEVSG
                     SSGTVIGSQDVVDLAGGDNLHIGGDGKDGVYVVVDASDGQVSLANNNSYLGTTQIASG
                     TLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHGRDIEMRADGEVA
                     VDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSAVRVEEGTLKGDV
                     ADIFPYASSLWVGDGAAFVTGADQDIQSIDATSSGTIDISDGTVLRLTGQDTSVALNA
                     SLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNTSGAVSLQNGVAG
                     DTLTVNGDYTGGGTLLLDSELNGDDSVSDQLVMNGNTAGNTTVVVNSITGIGEPTSTG
                     IKVVDFAADPTQFQNNAQFSLAGSGYVNMGAYDYTLVEDNNDWYLRSQEVTPPSPPNP
                     NPTPDPDPTPDPDPTPDPAPTPAYQPVLNAKVGGYLNNLRAANQAFMMERRDHAGGDG
                     QTLNLRVIGGRYHYTAAGQLAQQEDTSTVQLSGGLFSGRWGTDGEWMLGAVGSYSDNQ
                     GDSRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDNWLQYAWFSNDVSEHEDG
                     TDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVSQSQGDD
                     IQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKRRGEIKVGVTG
                     NISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(2976..3698)
                     /locus_tag="KLK57_003521"
     CDS             complement(2976..3698)
                     /locus_tag="KLK57_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HBE0893798.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            3845..6472
                     /gene="gyrA"
                     /locus_tag="KLK57_003522"
     CDS             3845..6472
                     /gene="gyrA"
                     /locus_tag="KLK57_003522"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HBE0893799.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVEGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            6680..>8183
                     /locus_tag="KLK57_003523"
     CDS             6680..>8183
                     /locus_tag="KLK57_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HBE0893800.1"
                     /translation="MVLLSAGVWALQYAGSGPEKTLSPLVVHNNLQIDLNEPDLFLDS
                     DSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIEGSIRRIVYEHDLTLKDKLFS
                     SLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPLLFAATSDSQLSKTEISSIKI
                     NSETIPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFKDDQQDTEATAIAGDLLSGKK
                     RWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMPSFAGVRFEMGNDGWHSFVAL
                     NDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAEEMLSHISQENDKLNGALDGA
                     AGLCWYEDSKLQTPLFVGQFDGTAEQAQLPGKLFTQNIGAHESKAPEGVLPISQTQQG
                     EAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLAMQNKTLLFSLDDTLVNNALQ
                     TLNKNRPAMVDVIPTDGIVPLYINSQGVAKLLRNETLTSLPKNLEPVFYNA"
BASE COUNT         1979 a         2029 c         2321 g         1854 t
ORIGIN      
        1 tgccgacgga aaaacgctgg ttattggcaa tacagagaat gacggagctg ttgactctat
       61 tgctggtacc gggttgatca ccaaaacagg ttccggcgat ctggtactta atgcagataa
      121 caatgacttt actggtgaga tgcagattga aaacggtgaa gttaccctgg gccgcagcaa
      181 ctccctgatg aatgtcggcg atacgcattg ccaggacgat ccgcaagact gctacggtct
      241 gacgataggg agtattgata agtaccagaa tcaggcagag ctaaacgttg gctccaccca
      301 acaaaccttt gtgcactcat tgacgggctt tcagaatggc actttaaata tcgatgctgg
      361 tggcaatgtt actgttaatc aaggcagttt tgctggcacc atcgaaggtg ctggtcagct
      421 caccattgcg caaaacggca gctacgtgct gtcgggggcg cagtcgatgg cgctaaccgg
      481 cgatatagtc gttgatgatg gtgcggtgct ttcgctggaa ggcgacgcgg cagatctcgc
      541 cgctctccag gacgatccgc agtcgatcgt gttaaacggc ggtgtgctcg atctctctga
      601 tttctccacc tggcagagcg gcacatcata caatgatggc cttgaagtca gtggcagcag
      661 cggaacggtt atcggcagtc aggatgtggt agatcttgca ggtggcgaca atttgcatat
      721 cggcggcgac gggaaagatg gcgtctacgt ggtggtcgat gcgagcgacg ggcaggtaag
      781 tctggcaaac aataatagtt atttgggcac aacacaaatc gcctccggta cgctgatggt
      841 gagcgacaac tcgcagcttg gagataccca ctataaccgc caggttatct ttaccgataa
      901 gcaacaagaa agcgtgatgg agattactgc caatgtcgat actcgctcta cgacgactga
      961 gcatgggcgt gatattgaaa tgcgcgccga cggtgaagtg gcagttgatg cgggggtaga
     1021 cacgcagtgg ggcgcactga tggctgacag cagcgggcag catcaggatg agggtagcac
     1081 atttactaaa acgggggcgg gtacgctgga gctgaccgcc agcggtacaa cgcagtcggc
     1141 agtacgtgtc gaagaaggca ccctgaaagg cgatgttgcg gatatctttc cttatgcttc
     1201 gtcactgtgg gtcggtgatg gggcggcgtt cgttactggc gcggatcagg atattcagtc
     1261 tatcgatgct acttccagcg gcactatcga tatcagcgat ggtacggttt tgcgtctgac
     1321 cgggcaggat acttccgtcg cccttaatgc ctcattgttt aacggcgatg ggacgctggt
     1381 gaatgctacc gatggcgtga cgttgacagg tgagcttaat accaaccttg aaactgacag
     1441 cctgacctat cttgccgacg tgacggttaa tggtgatctg accaatacgt ccggtgcggt
     1501 tagcctgcaa aatggcgtcg ctggcgatac gctgacggta aacggtgatt ataccggcgg
     1561 cggtacgcta ctgctcgata gcgaattaaa cggcgatgac tcggtaagcg atcaattggt
     1621 gatgaacggt aatactgctg gcaataccac ggtggtggtt aactctatta cagggattgg
     1681 tgagccgaca tcgacaggca ttaaagtggt tgatttcgca gctgatccca cgcagtttca
     1741 aaacaatgcg cagttcagtc tggcaggcag cggctacgtc aatatgggag cgtatgacta
     1801 cacgctggtg gaagataaca acgactggta tctgcgatcg caagaagtaa cgccgccatc
     1861 gccacctaat cccaacccga ctcccgatcc tgatcccaca ccggatcctg acccaacccc
     1921 cgacccggcc cctacgcctg cttaccagcc ggtgctgaat gccaaagttg gcggttatct
     1981 caataacctg cgggcggcaa atcaggcgtt tatgatggag cgacgcgatc acgctggtgg
     2041 cgatggtcag acgctgaatt tacgtgttat cggcggacgt tatcattaca cggcggcagg
     2101 gcaactggct cagcaggaag acacttctac ggtgcagctt agcggcggct tgtttagcgg
     2161 gcgctggggc acggatggcg agtggatgct tggggcggtt ggtagctaca gtgataatca
     2221 gggcgacagc cgctcgaata tgaccggaac tcgcgccgat aaccagaacc acggttatgc
     2281 cgttgggctg acctcaagct ggtatcagca cggtaatcag aagcaggggg cctggctgga
     2341 taactggttg cagtacgcgt ggtttagcaa tgatgtttct gaacatgaag atggcacaga
     2401 tcattaccat tcgtcgggga ttatcgcctc gctggaagcc gggtatcagt ggttaccggg
     2461 gcgtggtgtg gtgattgaac cgcaggcgca ggtgatttat cagggcgtgc agcaggatga
     2521 ttttaccgcc gctaaccatg cgcgcgtgtc acaatcgcag ggggatgata ttcagacgcg
     2581 gctgggttta cacagcgaat ggcgtaccgc ggttggtgtc acaccaacat tagatctgaa
     2641 ttattatcac gatccccatg cgacggaaat tgaagaagat ggcagcacta tcagtgacga
     2701 tgcggtgaag cgacggggtg aaataaaagt gggagtcacg ggcaatatca gtcagcgagt
     2761 gtccctgcgc ggcagcgtgg cgtggcagaa agggagtgat gattttgccc agacggcagg
     2821 gtttttgtcg atgacggtga aatggtaatc gctgtctgac catcacaatt gccggatgcg
     2881 gcgtaaacgc cttatctggc ctacggagag catttgtagg cctgataaga cgctgcaagc
     2941 gtcgcatcag gcattggcgc ggccaaacat caacctcact tattctgcgt gtgcagcata
     3001 tagttcacat ccacgccggg gccgagctta aaggaattag tgatcgggtt gtaatgtagc
     3061 ccggttatat gtcgctcttt taaactggtc tgatccaccc agccaagcag ctctgccggt
     3121 ttaataaact tcttcacatc atgcgtacct ttgggcacca tgcgcaaaat atattccgcg
     3181 ccaaccaccg ccatcagcca tgacttgccg ttgcggttaa gcgtcgagaa aaagacatcg
     3241 ccgcctggtt tcaccagttg cgcacaggct ctgaccactg actgcggatc gggtacgtgc
     3301 tccagcatct ccatgcaggt caccacatca tactgcccgg catgttttgc cgcgtgctct
     3361 tccacggttt cctgcacgta atccacctga atgccgcttt ccagcgcgtg cagctttgcc
     3421 acctgcaatg gctcaaagcc catatccaga ccggtcaccg tcgcgccttc gcgcgccata
     3481 ctctcggcca gaatgccgcc gccacaaccg acatcgagca cctttttgcc aaataaaccg
     3541 ccagcacgct cggcaatata gcccagacgc agcgggttaa tgcggtgcaa cggtttgaac
     3601 tcaccttcaa gatcccacca gcgggaggcg acggcttcaa atttagcgat ctcttcgtgg
     3661 tctacgttat ggttttccgg cgatttttcg gcattcattg gcacttctac tccgtaattg
     3721 gcaagacaaa cgagtatatc aggcattgga tgtgaataaa gcgtataggt ttacctcaaa
     3781 ctgcgcggct gtgttataat ttgcgacctt tgaatccggg atacagtaga gggatagcgg
     3841 ttagatgagc gaccttgcga gagaaattac accggtcaac attgaggaag agctgaagag
     3901 ctcctatctg gattatgcga tgtcggtcat tgttggccgt gcgctgccgg atgtccgaga
     3961 tggcctgaag ccggtacacc gtcgcgtact ttacgccatg aacgtactag gcaatgactg
     4021 gaacaaagcc tataaaaaat ctgcccgtgt cgttggtgac gtaatcggta aataccatcc
     4081 ccatggtgac ttggcggttt atgacacgat cgtccgtatg gcgcagccat tctcgctgcg
     4141 ttacatgctg gtagacggtc agggtaactt cggttccatc gacggcgact ctgcggcggc
     4201 aatgcgttat acggaaatcc gtctggcgaa aattgcccat gaactgatgg ccgatctcga
     4261 aaaagagacg gtcgatttcg ttgataacta tgacggcacg gaaaaaattc cggacgtcat
     4321 gccaaccaaa attcctaacc tgctggtgaa cggttcttcc ggtatcgccg taggtatggc
     4381 aaccaacatc ccgccgcaca acctgacgga agtcatcaac ggttgtctgg cgtatatcga
     4441 tgatgaagac atcagcattg aagggctgat ggaacacatc ccggggccgg acttcccgac
     4501 ggcggcaatc attaacggtc gtcgcggtat tgaagaagct taccgtaccg gtcgtggcaa
     4561 ggtgtacatc cgcgcccgtg ctgaagtgga agtcgacgcc aaaaccgggc gtgaaaccat
     4621 tatcgtccac gaaattccgt atcaggtaaa caaagcgcgc ctgatcgaga agattgcgga
     4681 actggtaaaa gaaaaacgcg tggaaggcat cagcgcgctg cgtgacgagt ctgacaaaga
     4741 cggtatgcgc atcgtgattg aagttaaacg cgatgcggtc ggtgaagttg tgctcaacaa
     4801 cctctactcc cagacccagt tgcaggtttc tttcggtatc aacatggtgg cattgcacca
     4861 tggtcagccg aagatcatga acctgaaaga tatcatcgcg gcgtttgttc gtcaccgccg
     4921 tgaagtggtg acccgtcgta ctattttcga actgcgtaaa gctcgcgatc gtgctcatat
     4981 ccttgaagca ctagccgtgg cgctggcgaa catcgacccg atcatcgaac tgatccgtca
     5041 tgcgccgacg cctgcagaag cgaaaactgc gctggtggct aatccgtggc agctgggcaa
     5101 cgttgccgcg atgctggaac gtgctggcga cgatgctgcg cgtccggaat ggctggagcc
     5161 agagttcggc gtgcgtgatg gtctgtacta cctgaccgaa cagcaagctc aggcgattct
     5221 ggatctgcgt ttgcagaaac tgaccggcct tgagcacgaa aaactgctcg acgaatacaa
     5281 agagctgctg gatcagatcg cggaactgtt gcgtattctt ggtagcgccg atcgtctgat
     5341 ggaagtgatc cgtgaagagc tggagctggt tcgtgaacag ttcggtgaca aacgtcgtac
     5401 tgaaatcacc gccaacagcg cagacatcaa cctggaagat ctgatcaccc aggaagatgt
     5461 ggtcgtgacg ctctctcacc agggctatgt taaatatcag ccgctttctg aatacgaagc
     5521 gcagcgtcgt ggcgggaaag gtaaatctgc cgcacgtatt aaagaagaag actttatcga
     5581 ccgactgctg gtggcaaaca cgcacgacca tattctgtgc ttctccagcc gtggtcgcgt
     5641 ctattcgatg aaagtttacc agttgccgga ggccactcgt ggcgcgcgcg gtcgtccgat
     5701 cgtcaacctg ctgccgctgg agcaggacga acgtatcacc gcgatcctgc cagtgaccga
     5761 gtttgaagaa ggcgtgaaag tcttcatggc gaccgctaac ggtactgtga agaaaaccgt
     5821 cctcaccgaa ttcaaccgtc tgcgtaccgc cggtaaagtg gcgatcaaac tggttgaagg
     5881 cgatgagctg atcggcgttg acctgaccag cggcgaagac gaagtaatgc tgttctccgc
     5941 cgaaggtaaa gtggtgcgct ttaaagagtc ttctgtccgt gcgatgggct gcaacaccac
     6001 cggtgttcgc ggtattcgct taggtgaagg cgataaagtc gtctctctga tcgtgcctcg
     6061 tggcgatggc gcaatcctca ccgcaacgca aaacggttac ggtaaacgta ccgcagtggc
     6121 ggaataccca accaagtcgc gtgcgacgaa aggggttatc tccatcaagg ttaccgaacg
     6181 taacggttta gtggttggcg cggtacaggt agatgactgc gaccagatca tgatgatcac
     6241 cgatgccggt acgctggtac gtactcgcgt ttcagaaatc agcatcgtgg gccgtaacac
     6301 ccagggcgtg atcctcatcc gtactgcgga agatgaaaac gtagtgggtc tgcaacgtgt
     6361 tgctgaaccg gttgacgagg aagatctcga taccatcgac ggcagtgccg cggaagggga
     6421 cgatgaaatc gctccggaag tggacgttga cgacgagcca gaagaagaat aattttactt
     6481 cttcatgcca aaagggagct atctcccttg tttgaattga aaagtccagg ctgcaaagtc
     6541 tgggcttttg tcgtattagg gcacggtaaa gtttggctgt gcccgtaaaa aatggctggc
     6601 tatacacaag gaatgtggca atgagtggtg aaaaaaagcg aaaggctggc ggttctatgg
     6661 tcttgtaggt tttggcgcaa tagtactgct ttccgctggt gtctgggcgt tgcaatatgc
     6721 tggcagtggg ccagaaaaaa cgttgtcgcc gctggtggtg cacaacaatc tgcaaatcga
     6781 tctcaatgag ccggacctct ttctcgacag cgactctctg agccagcttc ccaaagatct
     6841 cctcaccatt ccgtttctcc acgatgttct gagtgaagat ttcgttttct attatcagaa
     6901 tcatgccgat cgtctgggca ttgaaggcag cattcgtcgc attgtctatg aacacgatct
     6961 cacgctgaaa gataaactct tttcgtcact cttagatcag cccgcgcagg ctgcgctatg
     7021 gcacgataaa caaggccatc tttcacatta catggtgctg atccagcgca gtggtttaag
     7081 caaactgctg gagccattgt tgtttgccgc taccagcgac agccagttaa gcaaaacgga
     7141 aatcagtagc atcaagataa atagtgaaac tattcctgtt tatcagttgc gctataacgg
     7201 caataacgcc ctgatgttcg cgacttatca ggacaagatg ctggtgtttt ccagcacgga
     7261 tatgttgttt aaagatgatc agcaggatac cgaagccacg gcgatcgcag gtgatttgtt
     7321 gagcggcaaa aaacgctggc aagcaagctt tggcctggaa gagcgtgctg ctgaaaaaac
     7381 gccagtacgc cagcgcatcg tggtcagcgc caggttgctg ggttttggct accagcggtt
     7441 aatgccttct tttgctggcg tacgcttcga aatgggtaac gacggctggc acagttttgt
     7501 ggcgttaaat gatgaatccg ccagcgtaga taccagtttc gattttacgc cggtctggaa
     7561 cagtatgcct gccggggcca gcttctgtgt ggtggtgccg tattcacacg gtattgccga
     7621 agagatgctt tcgcacatca gccaggaaaa cgacaagctg aatggggcgt tagacggtgc
     7681 cgcggggctg tgctggtatg aagactcaaa attgcaaacc ccgctgtttg tcggtcagtt
     7741 tgatggcact gccgaacagg cgcaattgcc agggaaactg tttacgcaaa atattggtgc
     7801 gcacgaaagc aaagcgccag aaggtgtttt gccgataagc cagactcagc agggcgaagc
     7861 gcaaatctgg cgtcgcgaag tgagttcccg atacggtcag tatccgaaag cgcaggcggc
     7921 gcaacccgat caattaatgt cggattattt tttccgcgtg tcgctggcga tgcaaaacaa
     7981 aacgctgctt ttctccctcg atgacacgct ggttaataac gccctgcaaa cactgaataa
     8041 aaaccgcccg gcaatggtgg atgtaatacc cactgatggc atcgttccgc tctatatcaa
     8101 ttcacaaggc gtggcgaaac tgctgcgtaa cgaaacgttg accagtctgc cgaagaatct
     8161 cgaaccggtt ttttataacg ccg
//
LOCUS       DAEEKQ010000171         8157 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.173, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000171 DAEEKQ010000000
VERSION     DAEEKQ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.173"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..449)
                     /locus_tag="KLK57_003524"
     CDS             complement(<1..449)
                     /locus_tag="KLK57_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HBE0893801.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFN"
     gene            complement(566..1612)
                     /gene="ansB"
                     /locus_tag="KLK57_003525"
     CDS             complement(566..1612)
                     /gene="ansB"
                     /locus_tag="KLK57_003525"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HBE0893802.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            complement(1788..2507)
                     /locus_tag="KLK57_003526"
     CDS             complement(1788..2507)
                     /locus_tag="KLK57_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HBE0893803.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            complement(2691..3017)
                     /locus_tag="KLK57_003527"
     CDS             complement(2691..3017)
                     /locus_tag="KLK57_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HBE0893804.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="KLK57_003528"
     CDS             complement(3017..3736)
                     /gene="trmB"
                     /locus_tag="KLK57_003528"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HBE0893805.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            3897..4949
                     /gene="mutY"
                     /locus_tag="KLK57_003529"
     CDS             3897..4949
                     /gene="mutY"
                     /locus_tag="KLK57_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HBE0893806.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKTKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            4977..5252
                     /locus_tag="KLK57_003530"
     CDS             4977..5252
                     /locus_tag="KLK57_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HBE0893807.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            5317..6396
                     /gene="mltC"
                     /locus_tag="KLK57_003531"
     CDS             5317..6396
                     /gene="mltC"
                     /locus_tag="KLK57_003531"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HBE0893808.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLDGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            6598..7854
                     /gene="nupG"
                     /locus_tag="KLK57_003532"
     CDS             6598..7854
                     /gene="nupG"
                     /locus_tag="KLK57_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HBE0893809.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(7903..>8157)
                     /locus_tag="KLK57_003533"
     CDS             complement(7903..>8157)
                     /locus_tag="KLK57_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBE0893810.1"
                     /translation="IRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQR
                     YFLALEEGVNLLPGFSPELQGVYSETDADGMKRLYGYVLK"
BASE COUNT         1922 a         2159 c         1987 g         2089 t
ORIGIN      
        1 taattgaaat cttcgctatt cacgatcaga tatttattat tcgttttgat atcgtcgacc
       61 acccgcccac gatcgctggc tgccgcctga aaagagtaaa agttcaccga ccagccagac
      121 gcgggcgttc ctttactggt taacatcgcc gagaatttta ccggtttatc tttcgtccag
      181 gtctgttcga gcagttcgta atctgccaac tgaccgaccc aggtatagag atcgtcactg
      241 gaagagacat cgccttccgc tgaccaggtg gcctgattac ccttagcatc aagatttttt
      301 aaggtgatag catcaagctt gagaatgccg tgatactgct ttttgaattg atcttttaag
      361 atattttcag ttggcggttc gccatctgcc caggcattac cagtcatcaa cataagcagt
      421 gccgtgccaa caatccattg ctttttcacc cgaaaaacct cacgcggaat aatttattcc
      481 ggtgattatc attagggtaa tgaaaattgg caatggacgt aagtctgaag tgaaaaagcc
      541 ccggcacgat accggggcga ggtgattagt actgattgaa gatctgctgg atctgctgcg
      601 gatctttggt ttgcgtcaga gccagctgca gcaggacgcg cgctttttgc gggttcagcg
      661 tgccagaggc gacgaagccg tatttcgcat catccacttc agcatcctga gtggtagcac
      721 ccgtcggtac gcgggaagaa cgcactactg cagtgccgtt tttcgcggcg gttgccaggg
      781 tgtcgaacac ggatttatac aggttaccat tacccacacc agcgctaacg atgccatcat
      841 agcccgcatc taccagtgct ttagccggaa gatcggatgc gttagcgtag ttataaacga
      901 tgccgacttt cggcagctca ttcagcttag agacatcgaa tggcgtatcg ctggtgtgct
      961 tacgtgccgg ggtacgttgg tagtcaatct taccgttgtg aatgtatccc agaggaccgt
     1021 agttaacaga cttgaaggtc gctacgtcgg tggtgttggt tttggtgaca tcgcgaccgt
     1081 ccagtacggt gtcgttcatc accaccagca cgccacgatt agcggaggct ttatcagctg
     1141 cggtcactac cgcgttatac aggttgaatg gaccgtctgc gctcatggac gtggacgggc
     1201 gcattgcgcc gaccatcacc accggtttgt cgcatttcac cgtcaggtcg aggaagtaag
     1261 cggtttcttc catcgtgtcg gtaccgtggg taatgacgaa gccgtcggtt ttatcgcagt
     1321 cggtgttaat ttttttcgcc agtgtcagcc agacattatc gttcatgtcc tgggagccga
     1381 tattcactac ctgctcgcct ttaacgttcg caatgtcttt tagttgcggc accgcattaa
     1441 ccagattttc tacgccaact ttacccgctg tgtagttaga tttggttgcg gagtcaccac
     1501 caccggcaat ggtcccgccg gttgctaaaa tggtgatatt gggtaatgcc aatgctgcac
     1561 cactaaaacc cataaccagt gcggcaagtg ccgtcttttt gaaaaactcc atttcattcc
     1621 tccagttacg tgaacgctac gcattatccc ttagctctgt atgggaaatt tgacgttaaa
     1681 caatttacaa cgtgaatata ttttggagat ctacaaagtt agaggcaggt aacaaaacga
     1741 agaattaaac ggcataaaaa agtattatgc cgtcttaaaa tagaggatta ttttaaattc
     1801 ccgaccaggg ctttgcggct atcttccaga gtcacaacgc ggctacaaac atctttgcca
     1861 aactgctgga aatctttttc ctgctttttc cactctgttt ggattgagga ttgcagcccc
     1921 cccaggcttc ccagcacatt ctgtaatggg ttaccgccgc ttttcagcac cgctttcgcg
     1981 cccatttcat taatgctgtc ctgtaaaatt ccacccattg cctgattcac taattgctgg
     2041 ccttcggcgc gaacctgatc aatggcttta tagtgaaacg tcaggccatc gctgcgcgtt
     2101 tcgataatgc ggttcatctg ctctttcagc tgcgcatcaa gtttggtcag acggctgcgc
     2161 attttgctgc tttcgcccat ctcctgaacg ataattttat ccagcgcaat acgggctttt
     2221 tcgacgcggc ttttcgcgcc ttcatcaatc cacggcagcg tgctgcgtag ttcagcctga
     2281 taatccttcg cctgctcgcg ctgggcggca ttcagggaat attgcttacc gttatacatc
     2341 acgttgccgt ctggcgtgat caccagattg ccgttttcgc ccttcacctg cacggtttgc
     2401 gggctgacaa tcacatcgtc acgcggcgtg acgctgcact ggtagtcggc gtgagcggtc
     2461 attgccgtca ctgaaagtgc tgccgccagc agcattttgc gcatcatagt cttccctcaa
     2521 gaaaaaatca ggccagcatt tgctggcccc agattgataa caaagtacgc gttttccatg
     2581 ccggatgcgg cgtaaacacc ttatcccgcc tactcgatcg tgcatctttc caggcctgat
     2641 aagcgtagcg catcaggcag ttttgcattt gtcatcgccc gtatgctttc ttagtcccac
     2701 caaacgtcga aaagttcgct ggtgcgtacc tcatccagtt tgcgctcttc cagccactta
     2761 cgcacaatcg cctgatgttc ttcggtgcat ttgccgattt cctgcatgca gatcagacct
     2821 tcccaggcca gataaccgct gccgtcaaag gccagtttgt tcggttcgat aacctcgtta
     2881 ataaaatcat caacggtttt atcaatctgt tcttccgatg taccttccgg gaatcgccat
     2941 gccaccgaaa atcctaattc ctggaattcg tcgatgtgca tttttttacg cagacgacgg
     3001 ctacggttct ttgccattat ttcaccctct cgaacattaa gtcccatact ccgtgaccaa
     3061 gacgatgacc acgttgttca aatttcgtca ccggacgtga tgccggacgc ggcacgtaat
     3121 cattgctctc tgacaggttt ttataaccgt caatagaaga catcacttca agcatatgtt
     3181 ccgcataagg ttcccagtcg gtcgccatat ggaatacacc ccccagctgc agtttgcttt
     3241 ttaccagttc ggcaaacggc acctgtacga tacggcgttt attatggcgc gctttgtgcc
     3301 acgggtcagg gaaaaagagc tgcaccatgc gcaatgaatt gtcaggaatc attttatgca
     3361 gcacttcaac cgcatcgtga cacatcacgc gcaggttgct taaaccttct tcatgcgcag
     3421 aagccaggca cgcaccaacg cccggtgaat gcacttcaat gccgaggaag tcctgctcag
     3481 ggcgatcttt agccattgcc accagcgacg cccccatgcc aaaaccaatc tcaagcgtca
     3541 ccggcgcttc acggccaaaa agcgcgggga aatccagcat atcttcgctg aactcaacgc
     3601 ccatcaccgg ccagtagttt tccagcgcat gttcctggcc tttggtcagt cgcccctggc
     3661 ggcgcacaaa actacggata cggcgcagtg ggcggccgtt ttcatcaaat tccggtgaaa
     3721 tgacgtcgtt tttcataaag atttagtcgc ttgtgaaagt gttctgaaaa cgggcattat
     3781 ccaaagttag ttgccggatg caagcatgat aaggccgtgg ctgcggaaag ttccggttta
     3841 caccctgccg tcgctgtgct gcaatcttgc ccccaacaac agtgaattcg gtgaccatgc
     3901 aagcgtcgca attttcagcc caggttctgg actggtacga taaatacggg cgaaaaacgc
     3961 tgccctggca aattgacaag acgccctaca aagtatggct ctcagaagtg atgttgcaac
     4021 aaactcaggt tgcgaccgtt atcccctatt ttgaacgctt tatggcgcgc ttcccgacgg
     4081 tgaccgatct cgccaatgcg ccgctcgacg aagttctcca cttgtggacc gggcttggct
     4141 attacgcccg cgcgcgcaat ctgcataaag cggcacaaca agtggcgacc ttacacggcg
     4201 gtaaattccc ggaaaccttt gaagaagtcg cggcgttacc gggcgtcggg cgttccaccg
     4261 caggcgcgat tctctcgctt tctctgggta agcactttcc gattctcgac ggtaacgtca
     4321 aacgcgtgct ggcgcgctgc tatgctgtaa gcggctggcc tgggaaaaaa gaggtcgaga
     4381 ataaattatg gagtttgagc gagcaggtga cgcccgcggt cggcgtggaa cggtttaatc
     4441 aggcgatgat ggatttgggc gcgatgattt gtacgcgctc gaagccgaaa tgttcgctct
     4501 gtccgctaca aaacggatgt attgccgccg ccaataatag ctggtcgctt tatccgggca
     4561 aaaaaacgaa acagacgctg ccggagcgca ccggctactt tttgctgtta cagcacgaag
     4621 atgaagtatt gctggcgcag cgtccgccga gcggattgtg gggcggttta tactgtttcc
     4681 cgcagtttgc cgacgaagaa agtttgcggc agtggctggc gcaacggcag attgctgccg
     4741 ataacctgac gcaactgacc gcgtttcggc ataccttcag ccatttccac ttagatattg
     4801 tgcctatgtg gcttcccgtg tcgtcattca ccggctgcat ggatgaaggc aatgcgctct
     4861 ggtataactt agcgcaaccg ccgtcagttg gcctggcggc tcccgtggag cgtttgttac
     4921 agcagttacg cactggcgcg ccggtttagc gcgtgagtcg ataaagagga tgatttatga
     4981 gcagaacgat tttttgtact ttcctgcaac gtgaagcaga aggtcaggat tttcagctgt
     5041 accccggcga gctgggaaaa cgcatctata acgagatctc caaagaagcc tgggcgcagt
     5101 ggcagcacaa gcaaaccatg ctgattaatg aaaagaaact caacatgatg aatgccgagc
     5161 accgcaagct gcttgagcag gagatggtca acttcctgtt cgagggtaaa gaggtgcata
     5221 tcgagggcta tacgccggaa gataaaaaat aaaaacagtg ccggagcacg cctccggcaa
     5281 cttgcataaa aacaaacaca acacgcaccc ggaatgatga aaaaatatct cgcgctggct
     5341 ttgattgcgc cgttgctcat ctcctgttcg acgaccaaaa aaggcgatac ctataacgaa
     5401 gcctgggtca aagataccaa cggttttgat attctgatgg ggcaatttgc ccacaatatt
     5461 gagaacatct ggggattcaa agaggtggtg atcgccggtc ctaaggacta cgtgaaatac
     5521 accgatcaat atcagacccg cagccacatc aacttcgatg acggtacgat tactatcgaa
     5581 actatcgccg ggacagaacc tgccgcgcat ttgcgccggg caattatcaa aacgttgctg
     5641 atgggtgacg atccgagttc ggtcgatctc tattccgacg ttgatgatat tacgatttcg
     5701 aaagaacctt tcctttacgg tcaggtggtg gacaacaccg ggcagccgat tcgctgggaa
     5761 ggtcgcgcaa gcaacttcgc ggattatctg ctgaaaaacc gtctgcaaag ccgcagtaac
     5821 gggctgcgta tcatctacag cgtcaccatt aacatggtac cgaaccacct tgataaacgt
     5881 gcgcacaaat atctcggcat ggtccgccag gcgtcacgga aatatggcgt tgatgagtcg
     5941 ctgattctgg caattatgca gaccgaatct tcctttaacc cgtatgcggt cagccgttcc
     6001 gatgcgctgg gattaatgca ggtggtacaa catactgccg ggaaagatgt gttccgctcg
     6061 caggggaaat ccggcacgcc gagccgcagt ttcttgtttg atcctgccag caatattgat
     6121 accggcaccg cgtatctggc gatgctgaac aatgtttatc tcgacggaat tgataaccca
     6181 acatcgcggc gttatgccgt catcaccgcc tataacggcg gcgcaggcag cgtgctgcga
     6241 gtcttttcga atgataagat tcaggctgcc aatattatta acaccatgac gccgggcgat
     6301 gtttatcaga cgctgacgac ccgccatccc tctgcggaat ctcgccgtta tctttataaa
     6361 gtgaataccg cgcaaaaatc ctaccgccgc cgataattcc attaaccgcc cctgacgatg
     6421 ctcaggggca aaaatgttat ccacatcaca atttcgtttt gcaaattggg aatgtttgca
     6481 attatttgcc acaggtaaca aaaaaccagt ccgcgaagtt gatagaatcc catcatctcg
     6541 cacggttaaa tgtgcttttt caaacactca tccgcatcac aatgtgagga aattaacatg
     6601 aatcttaagc tgcagctgaa aatcctctct tttctgcagt tctgtctgtg gggaagttgg
     6661 ctgacgaccc tcggctccta tatgtttgtt accctgaagt ttgacggtgc ttctattggc
     6721 gcagtttata gctcactggg tatcgccgcg gtatttatgc ctgcgctgct ggggattgtg
     6781 gccgacaaat ggttaagtgc gaaatgggta tatgccattt gccacaccat tggcgctatc
     6841 acgctgttca tggcggcaca ggtcacgacg ccggaagcga tgttccttgt gatattgatt
     6901 aactcgtttg cttatatgcc aacgcttggg ttaatcaaca ccatctctta ctatcgcctg
     6961 caaaatgccg ggatggatat cgttactgac ttcccgccaa tccgtatctg gggcaccatc
     7021 ggctttatca tggcaatgtg ggtggtgagc ctgtctggct tcgaattaag ccacatgcag
     7081 ctgtatattg gcgcagcact ttccgccatt ctggttctgt ttaccctgac tctgccgcat
     7141 attccggttg ctaaacagca agcgaatcag agctggacaa ccctgctggg cctcgatgca
     7201 ttcgcgctgt ttaaaaacaa gcgtatggca atcttcttca tcttctcaat gctgctgggc
     7261 gcggaactgc agattaccaa catgttcggt aataccttcc tgcacagctt cgacaaagat
     7321 ccgatgtttg ccagcagctt catcgtgcag catgcgtcaa tcatcatgtc gatttcgcag
     7381 atctctgaaa ccctgttcat tctgaccatc ccgttcttct taagccgcta cggtattaag
     7441 aacgtaatga tgatcagtat tgtggcgtgg atcctgcgtt ttgcgctgtt tgcttacggc
     7501 gacccgactc cgttcggtac tgtactgctg gtactgtcga tgatcgttta cggttgcgca
     7561 ttcgacttct tcaacatctc tggttcggtg tttgtcgaaa aagaagttag cccggcaatt
     7621 cgcgccagtg cacaagggat gttcctgatg atgactaacg gcttcggctg tatcctcggc
     7681 ggcatcgtga gcggtaaagt tgttgagatg tacacccaaa acggcattac cgactggcag
     7741 accgtatggt tgattttcgc tggttattcc gtggttctgg ccttcgcgtt catggcgatg
     7801 ttcaaatata aacacgttcg tgtcccgaca ggcacacaga cggttagcca ctaattacgc
     7861 aaagaaaaac gggtcgccag aaggtgaccc gtttttttat tcttacttca acacataacc
     7921 gtacaatcgt ttcatgccat ccgcatcggt ttcgctatag acaccttgca actccggcga
     7981 aaatcccggc aacaaattca cgccttcttc cagcgcaagg aaataacgtt gaaccgcccc
     8041 accccagact tccccgggaa ccacgcaaag cacgccaggt ggataaggca acgccccttc
     8101 tgccgcaatt cgcccttcgg catcacgaat ccgcaccaac tccacttcac cgcgaat
//
LOCUS       DAEEKQ010000172         8127 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.174, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000172 DAEEKQ010000000
VERSION     DAEEKQ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8127)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8127)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8127
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.174"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            167..321
                     /locus_tag="KLK57_003534"
                     /pseudo
     CDS             167..321
                     /locus_tag="KLK57_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mRNA interferase"
     gene            367..783
                     /gene="hicB"
                     /locus_tag="KLK57_003535"
     CDS             367..783
                     /gene="hicB"
                     /locus_tag="KLK57_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HBE0893811.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            862..2268
                     /locus_tag="KLK57_003536"
     CDS             862..2268
                     /locus_tag="KLK57_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HBE0893812.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESQGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRE
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKCLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            2513..3658
                     /locus_tag="KLK57_003537"
     CDS             2513..3658
                     /locus_tag="KLK57_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HBE0893813.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            3676..4689
                     /locus_tag="KLK57_003538"
     CDS             3676..4689
                     /locus_tag="KLK57_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HBE0893814.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVQQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            4690..5590
                     /locus_tag="KLK57_003539"
                     /pseudo
     CDS             4690..5590
                     /locus_tag="KLK57_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            5580..6374
                     /locus_tag="KLK57_003540"
     CDS             5580..6374
                     /locus_tag="KLK57_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0893815.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGSVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     LLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            6396..7820
                     /gene="patD"
                     /locus_tag="KLK57_003541"
     CDS             6396..7820
                     /gene="patD"
                     /locus_tag="KLK57_003541"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HBE0893816.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHAASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            8030..>8127
                     /locus_tag="KLK57_003542"
     CDS             8030..>8127
                     /locus_tag="KLK57_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HBE0893817.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNK"
BASE COUNT         1921 a         1908 c         2222 g         2076 t
ORIGIN      
        1 attattcagt tttgcaaatt agcgcaaaga aattctggaa tcttccttcc tgattttgca
       61 ttgcattctg ccgttgcggc gatttagtgc tattttcgag caaattacac ataggggtaa
      121 acactaatgt ttactttatt gatatactca gcggcaggga ggcgatgtga aacaaagcga
      181 gttcagacgt tggctcgaat ctcagggcgt cgatgtagcg aatggccgca accatttgaa
      241 actcaggttt catgggaggc gcagtgtcat gccgcgtcac tcctgcgtaa agcaatcctg
      301 aaacaactcg gtttgagtta atcgccaatt aaaaaggtta atgacatgcg agagacagtc
      361 gaaattatgc gttatcccgt cactcttaca cccgcgccgg aaggcggtta tatggtttct
      421 tttgtggata tccctgaagc gttgacgcag ggcgaaactg tcgctgaagc gatggaagcg
      481 gcaaaagatg ctttactgac cgcatttgat ttttattttg aagataacga gcttatcccg
      541 ttaccttcgc cattaaatag tcatgatcac tttattgaag tacctttgag cgtcgcctct
      601 aaagtattgc tgttaaatgc ttttttacag tcagaaatca ctcagcaaga gttagccagg
      661 cgaattggca aacctaaaca ggagattact cgcctattta acttgcacca tgcgacaaaa
      721 atcgacgccg tccagcttgc ggcaaaggcg cttggcaaag agttatcgct ggtgatggtt
      781 taattacagt taacgaaaag ttgtcatttt taacaactga tatagactgc cgaatcatct
      841 gcacataatt acgattcgat aatgaaaaaa taccagcagc ttgcagaaca attacgcgag
      901 cagattgcgt cgggtatctg gcaacccggc gatcgtttgc cttcgttgcg tgaccaggtg
      961 gcgctttcag gcatgagctt tatgactgtc agccatgcct atcagttgct cgaaagtcag
     1021 ggatatatta tcgcacgacc gcagtcgggt tattacgttg cgccacaggc aataaaaatg
     1081 ccgaaagcgc cagtcattcc agtcactcga gatgaagcag tcgatatcaa cacttatatt
     1141 tttgatatgt tgcaggccag tcgcgaaccg tcggtcgttc cgtttgcctc ggcctttccc
     1201 gacccgcgac ttttccccct ccaacaacta aaccgctcgc tggcgcaggt aagcaaaacc
     1261 gccacagcga tgagcgtgat tgaaaactta ccgccaggaa acgcagaact gcgtcaggct
     1321 attgctcgtc gctatgcctt acagggcatc accatttctc ctgatgaaat tgtcattact
     1381 gccggggcgt tagaggcatt aaacctcagt ttgcaagcgg taactgaacc gggcgattgg
     1441 gtgatagtag agaatccttg tttctacggt gcgttgcagg cgctggagcg gctacggctg
     1501 aaggcgttat cggtggcgac ggatgttaaa gaagggatcg atcttcaggc gctggaactg
     1561 gcgttgcagg attatccggt gaaagcgtgc tggctgatga ctaatagcca gaacccactc
     1621 ggatttacct taacgccgca aaaaaaagca caactggtgg cgttactcaa tcagtacaac
     1681 gtaacgctga ttgaagatga cgtttacagc gaactttatt ttggacggga aaaaccgctg
     1741 cctgcgaaag cgtgggatcg ccacgatggc gttttgcatt gctcttcgtt ttcgaaatgt
     1801 ctggtgcctg gttttcgtat tggttgggtc gccgccggaa aacatgcacg taaaattcaa
     1861 cgcttgcagt tgatgagtac gctttccacc agctcaccga tgcaacttgc gctggtggat
     1921 tacctttcca cgcgccgata cgacgcccat cttcgtcgcc tgcgtcgcca gcttgcggaa
     1981 cgtaaacaac gtgcctggca ggcactgctg cgttatctgc ctgcggaagt taaaattcat
     2041 catagtgaca gcggttattt tctctggctg gagctccccg agccgttaga tgccggcgaa
     2101 ttaagcctgg cggcactgac gcatcatatc agtattgcgc cgggtaaaat gttttctacc
     2161 ggtgaaaact ggtcacgttt ttttcgtttt aataccgcgt ggcagtgggg agaacgtgaa
     2221 gaacaggcgg taaaacaatt aggcaaactt attcaagaac ggctgtaata gcgtttaatt
     2281 taattcctct tagattgggt aatatgaatt tcgaatagca gtcatatttc ctaacccctt
     2341 gtctatactc cagaagataa ccttacagac ggcataatgc gcggtagctc acaacctgat
     2401 taaattttct cagggacgaa ggtgtgcctg caagccgccg tctatggtta aacaaggaga
     2461 tatttttacg gcacggcggc tgaacaatta attacgacag gagtaagacc ttatgagcaa
     2521 gacatttgcc cgcagcagcc tgtgtgcgct caccatgaca ataatgaccg ctcacgccgc
     2581 cgaaccacct accaatttag ataaaccgga agggcgactg gatattatcg cctggccggg
     2641 atacatcgaa cgcggacaaa ctgataaaca atacgactgg gtaacgcaat ttgaaaaaga
     2701 gacaggctgc gcggtgaatg tgaaaaccgc cgcgacttcc gatgaaatgg tcagtctgat
     2761 gaccaaaggg ggttacgatc tggttacggc atccggcgat gcctcgctgc gtttgattat
     2821 gggcaaacgc gtgcagccga ttaataccgc cttgattccc aactggaaaa cgctcgatcc
     2881 gcgcgtggtt aaaggcgact ggtttaatgt tggcggcaaa gtttacggca caccttacca
     2941 atgggggccg aacctgctga tgtacaacac taaaaccttc ccgacgccgc cggatagctg
     3001 gcaagtggtt tttgttgagc aaaatctgcc ggacggcaag agcaataaag gccgcgttca
     3061 ggcttatgat ggccctatct acattgcgga cgctgcgttg ttcgttaaag ccactcagcc
     3121 gcagttgggc atcagcgatc cgtatcaact aaccgaagaa cagtaccagg cggtgctgaa
     3181 agtgctgcgc gctcaacaca gtttgatcca tcgctactgg catgacacta ccgtgcaaat
     3241 gagcgatttc aaaaacgagg gtgtggttgc ttccagtgcc tggccctatc aggccaacgc
     3301 cctgaaagcc gaaggccagc ctgttgctac cgttttcccg aaggagggtg ttaccggttg
     3361 ggctgatacc accatgctgc atagcgaagc gaaacatccg gtttgcgcct acaaatggat
     3421 gaactggtca ttaacgccaa aagtgcaggg cgatgtggcg gcctggtttg gctcgttacc
     3481 ggtagtgccg gaagggtgta aagccagtcc gttattaggc gaaaaaggtt gtgaaaccaa
     3541 cggttttaac tatttcgaca aaatcgcctt ctggaaaacg cctatagcag aagggggcaa
     3601 gtttgttccc tacagtcgct ggacgcagga ttacattgcc attatgggcg gtcgctaact
     3661 tcgctggggt gctttatgac gtacgcagtg gagtttgaca acgtctcgcg gttgtacggt
     3721 gacgtgcgcg cagtagatgg cgtcagtatt gcgataaaag atggtgagtt cttctctatg
     3781 ctggggccgt ccggctccgg caaaaccacc tgcctgcgcc tgattgctgg cttcgaacag
     3841 ctttccggcg gggctatctc tatctttggt aaacccgcca gcaatctgcc accgtgggag
     3901 cgggacgtga atactgtctt tcaggactac gcgctatttc cgcatatgtc gattcttgac
     3961 aatgtcgcct atgggctgat ggtcaaaggc gtgaataaaa agcagcggca cgcaatggcg
     4021 caagaggcgc tggagaaagt ggcgttgggg tttgtacagc aacgtaaacc gtcacaactt
     4081 tctggtggtc agcgccagcg ggttgctatc gccagagcat tggtgaatga accgcgcgta
     4141 ttgctgttgg atgaaccgct cggcgcactg gatctcaaat tgcgtgagca gatgcagctg
     4201 gaactgaaaa aactgcaaca gtctctcggt atcactttta tcttcgttac ccacgatcag
     4261 ggcgaagcgt tatcgatgtc cgatcgtgtg gcggttttca ataatggacg cattgagcag
     4321 gtcgattccc cgcgcgatct ctatatgcgc ccgcgcacgc cgtttgttgc cgggttcgtt
     4381 ggtacatcga atgtttttga tggactgatg gcagagaaac tttgtggcat gacgggaagc
     4441 ttcgccctgc gaccggaaca tatccgcctc aacacccctg gtgaactgca ggccaatggc
     4501 acgatccagg cggtgcaata tcagggcgcg gcaactcgtt ttgaactgaa attgaacggc
     4561 ggtgaaaaac tgcttgtgag tcaggccaat atgacaggcg aagaactgcc tgccacgctc
     4621 acgcccggac aacaggtgat ggtttcctgg tcgcgtgatg tgatggtgcc gctggttgag
     4681 gagaggtgaa tggcgatgaa tgtattgcaa tcaccttcac gtccaggtct ggggctgttt
     4741 ttactgctgc ttggcccgct aatgtggttt ggcattgtct atttcggctc gctgctgaca
     4801 ctgttatggc agggatttta tacttttgac gatttcacca tgtcggtaac gccggaactg
     4861 acgctggcga atatccgtgc gctgtttaat ccggcgaatt acgacatcat tctccgcacg
     4921 ctgactatgg ctgtggcagt cactatcgcc agcgccattc tggcttttcc aatggcgtgg
     4981 tatatggcgc gctataccag cgggaaaatg aaagcgtttt tatattgcgg taatgttgcc
     5041 gatgtgggcg agctacattg ttaaagccta tgcctggacg ttattgctgg caaaagatgg
     5101 cgtggctcag tggtttttac aacatcttgg gctggaacca ctgctgactg cgttccttac
     5161 attacctgcg gtgggcggaa atacgctgtc aacttccggg ctggggcgct ttctggtgtt
     5221 tctctatatc tggttgccgt tcatgatcct gcccgttcag gcggcgcttg agcgtttgcc
     5281 gccgtcattg ttgcaggcgt cggctgatct cggcgcacgt ccacgacaaa cctttcgcta
     5341 tgtggtgctg ccgctggcaa tcccgggtat tgccgctggc tctatcttta ccttctcact
     5401 cactctgggc gattttatcg tcccgcagct ggttggtcct ccaggatatt ttatcggcaa
     5461 tatggtttat tcccagcagg gggcgattgg caatatgccg atggcggcgg cattcacctt
     5521 ggtgccgatt attctcatcg cactgtacct ggcgttcgtg aaacgtctgg gagcgttcga
     5581 tgcactctga acgcgcaccg tttttcctca aactggcggc ctggggcggc gttgttttcc
     5641 tacattttcc catcctgata atcgccgcct atgcgtttaa cactgaagat gcggcgttta
     5701 gttttccacc gcagggcctg acgctgcgct ggtttagcgt ggcagcacag cgtagtgata
     5761 ttcttgatgc cgtgacactg tcacttaaag tggcggcgct ggcgacatta attgcgctgg
     5821 tgttagggac gctggcagct gccgcgctgt ggcgacgaga ctttttcggc aaaaacgcca
     5881 tttcgctgtt actgctgctg cccattgcgc tgccgggcag cgtcactggt ctggcgttat
     5941 taaccgcctt taaaaccatc aatctggagc cgggattttt caccatcgtg gtcggtcatg
     6001 cgactttttg tgtagttgtg gtgtttaaca atgtcatcgc ccgttttcgc cgcacctcct
     6061 ggagtctggt tgaggcgtca atggatcttg gggccaatgg ctggcaaacc ttccgctacg
     6121 tagtgttgcc gaatctcagt tcggcgttac tggcaggagg aatgctggcg tttgccttgt
     6181 cgttcgatga aatcatcgtt acgaccttta cggcaggtca tgaacgaacg ttactgttgt
     6241 ggttgctcaa tcagcttggg cgaccgcgtg atgtaccggt aactaacgtg gtggcactgc
     6301 tggttatgtt ggtaacaacc ttgccgatcc tgggggcctg gtggctaacc cgcgaaggcg
     6361 acaatggtca ataaccactg atacaggaat atgctatgca acataagtta ctgattaacg
     6421 gagaactggt tagcggcgaa ggggaaaaac agcctgtcta taatccggca acgggggacg
     6481 ttttactgga aattgccgag gcatccgcag agcaggtcga tgctgctgtg cgcgcggcag
     6541 atgcagcatt tgccgaatgg gggcaaacca cgccgaaagt gcgtgcggaa tgtctgctga
     6601 aactggctga tgttatcgaa gaaaatggtc aggtttttgc cgaactggag tcccgtaatt
     6661 gtggcaaacc gctgcatagt gcgttcaatg atgaaatccc ggcgattgtc gatgtttttc
     6721 gctttttcgc gggtgcggcg cgctgtctga atggtctggc ggcaggtgaa tatcttgaag
     6781 gtcatacttc gatgatccgt cgcgatccgt tgggggtcgt ggcttctatc gcaccgtgga
     6841 attatccgct gatgatggcc gcgtggaaac ttgctccggc gctggcggca gggaactgcg
     6901 tagtgcttaa accatcagaa attaccccgc tgaccgcgtt gaagttggca gagctggcga
     6961 aagatatctt cccggcaggc gtgattaaca tactgtttgg cagaggcaaa acggtgggtg
     7021 atccgctgac cggtcatccc aaagtgcgga tggtgtcgct gacgggctct atcgccaccg
     7081 gcgagcacat catcagccat gccgcgtcgt ccattaagcg tactcatatg gaacttggtg
     7141 gcaaagcgcc agtgattgtt tttgatgatg cggatattga agcagtggtc gaaggtgtac
     7201 gtacatttgg ctattacaat gctggacagg attgtactgc ggcttgtcgg atctacgcgc
     7261 aaaaaggcat ttacgatacg ctggtggaaa aactgggtgc tgcggtggca acgttaaaat
     7321 ctggtgcgcc ggatgacgag tctacggagc ttggaccttt aagctcgctg gcgcatctcg
     7381 aacgcgtcag caaggcagta gaagaggcga aagcgacagg gcacatcaaa gtgatcactg
     7441 gcggtgaaaa gcgcaagggt aatggctatt actatgcgcc gacgctgctg gctggcgcat
     7501 tacaggacga tgccatcgtg caaaaagagg tatttggtcc agtagtgagt gttacgccct
     7561 tcgacaacga agaacaggtg gtgaactggg cgaatgacag ccagtacgga cttgcatctt
     7621 cggtatggac gaaagatgtg ggcagggcgc atcgcgtcag cgcacggctg caatatggtt
     7681 gtacctgggt caatacccat ttcatgctgg taagtgaaat gccgcacggt gggcagaaac
     7741 tttctggtta cggcaaggat atgtcacttt atgggctgga ggattacacc gtcgtccgcc
     7801 acgtcatggt taaacattaa gttagatgtt agtggctgtt ataggacggc cactatcgtt
     7861 ttaggttctg agtttaatgg cggttaagta ttagaatgat tacattaata actagcgtaa
     7921 aaattaggct aaatgtttga tggcgatttt tcatcctgtc acagttgatt agcataaggc
     7981 tttagagtat cgttattaaa ctaataattt ataagtagaa gtattaacaa tgaaagttca
     8041 attgtacaaa ttcacggaag ataaaaataa aacccttacc ttcagatgga cgaaaaaaca
     8101 ctttgagttt tgtatggata ataaaat
//
LOCUS       DAEEKQ010000173         7968 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.175, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000173 DAEEKQ010000000
VERSION     DAEEKQ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7968
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.175"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..239
                     /locus_tag="KLK57_003543"
     CDS             <1..239
                     /locus_tag="KLK57_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HBE0893818.1"
                     /translation="LLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIVQLLLEHG
                     ASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            279..1142
                     /locus_tag="KLK57_003544"
     CDS             279..1142
                     /locus_tag="KLK57_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HBE0893819.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQTVNDKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPAEKYKEEFYRGLLRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYGVPGNSTQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            1132..2679
                     /gene="fdrA"
                     /locus_tag="KLK57_003545"
     CDS             1132..2679
                     /gene="fdrA"
                     /locus_tag="KLK57_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HBE0893820.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPTLEQAKNGDLMIVINGKSGADNEQLLVEIEELFNTKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAVLKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            2679..4037
                     /locus_tag="KLK57_003546"
     CDS             2679..4037
                     /locus_tag="KLK57_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HBE0893821.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAGPPITWEKMCGAMKGAVTGAL
                     VFEGLAKDLDEAAELAASGEITFSPCHEHDCVGSMAGVTSASMFMHIVKNKTYGNIAY
                     TNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDAMKIIGEIDLRLMLAQALHMG
                     DECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEFVASSDYFSGPTWMAMCKAAM
                     DAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPAQQVIGPMFAGYKPEDSGLDI
                     GDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSRQMREITLGENPNVTIPLLGF
                     MGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAGIVHPPFACFEKAILGWCERY
                     GV"
     gene            4180..5130
                     /locus_tag="KLK57_003547"
     CDS             4180..5130
                     /locus_tag="KLK57_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HBE0893822.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADMVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTDVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFSPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            5140..6522
                     /locus_tag="KLK57_003548"
     CDS             5140..6522
                     /locus_tag="KLK57_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HBE0893823.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTYVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLKQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            6899..7948
                     /gene="yahK"
                     /locus_tag="KLK57_003549"
     CDS             6899..7948
                     /gene="yahK"
                     /locus_tag="KLK57_003549"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HBE0893824.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMIGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAEKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIADSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
BASE COUNT         1919 a         2018 c         2308 g         1723 t
ORIGIN      
        1 agcttttggc gcatacggag attaacgtta accagaccaa ccatgtaggc tggacgccgc
       61 tactggaagc gattgtgctt aatgatggtg gtattaaaca gcaggcgatt gtgcagttat
      121 tgctggaaca cggtgccagc ccgcatctga ccgataaata tggcaaaacg ccactggaac
      181 tggcgcggga acggggcttt gaagagattg cgcagttact gattgccgca ggtgcataaa
      241 ccgggaggct ggctatcaac acaccagaaa aacggtgtgt gtgggcgcta actgcggatg
      301 cggattttct ggcgcagcgg gggcaaggac aggttgaaca ggtctttgcc agagcggtaa
      361 atatcgcact cccggctcgc cagcagttgc tgacgctgct ttgtgaagag tacgacaatg
      421 cgccaaacag ttgtcggttg gcactcactc actttgatga tctgttccgg catggtgata
      481 aggttcagtt tgacgatcaa ggtattacgg ttggtcaaca tcttcatata gagatgagtc
      541 gttgtcggcg ttggctgtcc ccaaccttgc aaatgaccgc tgtgaatttt caccttatcg
      601 cctggctaca gtggcacgac attattcatc agcacctggg ggaaaatgaa accctgttta
      661 attatcgcgg cgataatccg ttttatcagg cgttaaataa agaattacat attaaacgac
      721 gggcagttat tcagacagta aacgataagc aaaatatcgc cgcagcggtc gccagtatga
      781 ttgggttagg gattggcctt acgccatcag ccgatgatta tttaacaggt ctggcgctta
      841 ttttatttat tcccgggcat ccggcagaaa aatacaaaga ggaattttat cgcggtctgc
      901 tacgcggcag aaataacacc acgttattaa gtgccataac gctggaagcc gcattacaac
      961 aacgctgccg ggaaaatatt catcgcttta ttcacaacat tatttatggc gtccctggga
     1021 attcaactca ggcaatagaa aaaattaaac atattggctc cagttccggc tgcgacatgc
     1081 tgtatggcat ggccgatggt tgtgcgctga gccaaaccta cggagggaat tatgtcagtt
     1141 aaaatagtca ttaaaccgaa tacctatttt gattctgtct cgctgatgtc tatctccacg
     1201 cgtgcaaata aactcgacgg cgtcgagcag gcatttgtgg cgatggcgac cgaaatgaac
     1261 aaaggtgtgc tgaaaaattt aggactgctg acgccgacgc tggaacaggc gaaaaacggc
     1321 gacctgatga ttgtcatcaa tggtaaatcg ggtgcggaca acgagcagtt gctggtggag
     1381 attgaagaac tgttcaacac caaagcgcaa agcggctcgc acgaggcgcg ttacgccact
     1441 attgccagcg ccaaaaagca tatcccggaa agtaacctgg cggtgatttc ggtcaacggt
     1501 ctgtttgccg ctcgcgaagc gcgtcaggcg ctgcaaaacg atctcaacgt gatgctgttt
     1561 tccgataacg tctcagttga agatgaactg gcgctcaagc agttggccca cgaaaaaggg
     1621 ctgctgatga tggggccgga ctgcggcacg gcgattatta acggcgcggc gctctgtttt
     1681 ggtaacgccg tgcgtcgcgg caatatcggt attgttggcg catccggcac cggcagccag
     1741 gagctgagcg tccgcattca tgaatttggt ggcggtgttt cacaactgat tggcaccggc
     1801 gggcgcgacc tgagcgagaa aatcggcggc ctgatgatgc tcgacgccat cgggatgctg
     1861 gaaaacgatc cgcaaactga aattatcgca ctgatttcaa aacctcccgc gcctgcggtg
     1921 gcccgcaaag tgctggaacg cgcacgtgcc tgccgcaagc cggtggtggt ctgcttcctc
     1981 ggtcgcgttg aaacgccggt ggatgagcag gggctacagt ttgcccgcgg cagcaaagag
     2041 gcggtgctaa aagcggtgat gctctccggc gtgaaacagg aaaatctcga cctgcatacg
     2101 cttaaccagc cgttgattgc tgatgtgcgt gcgcgtctgc aaccgcagca gaaatacatt
     2161 cgtggcctgt tctgcggcgg cacgctgtgc gacgaaacca tgttcgcggt gatggaaaaa
     2221 catggcgatg tctacagcaa cattcagccc gatccggaat tccgcctgca agatatcaac
     2281 cgcagcatca aacacacctt cctcgacttt ggcgatgacg acttcacgaa cggcaagccg
     2341 catccaatga ttgaccccac caaccgcatc agtcgcttga tcgaagaggc gcgcgatcca
     2401 gaagtggcgg tgatcgtgat ggattttgtg ctcggatttg gatcgcatga agatccggtc
     2461 ggctccacca tcgaggcgat caaagaagcg aaagcgatcg ccgctgccga aggacgcgag
     2521 ttgatcattc tcgcctatgt gctgggtacc gatcttgata cgccatcgtt agaacaacaa
     2581 agccagatgc tgcttgatgc cggagtgatt ctggcgagca gcagcaccaa taccggattg
     2641 ctggcgcgtg aatttatctg caaaggggag gaagcctgat gagccagtca ctgtttagcc
     2701 aaccattgaa cgttattaac gtcggcatcg ccatgtttag cgatgacctg aaaaagcagc
     2761 atgtagaagt gactcaactc gactggacgc cgccggggca gggcaatatg caggtggtgc
     2821 aggcgctgga gcgcattatc cagtcgcatc cggtgctgat tggttttgat caggcgatta
     2881 acgtggtgcc gggcatgacg ccgaaaacca ttcttcacgc cgggccgccg atcacctggg
     2941 aaaaaatgtg cggcgcgatg aaaggcgcgg tcaccggagc gctggtgttc gaaggactgg
     3001 cgaaagatct cgacgaggcg gctgaactgg cggcctccgg ggagatcacc ttctcgccgt
     3061 gccacgaaca cgactgtgtg ggatcgatgg cgggcgttac ctcggcctcg atgtttatgc
     3121 acatcgtgaa aaacaaaacc tacggcaaca tcgcttacac caatatgagc gagcagatgg
     3181 cgaagatttt gcgtatgggt gccaacgatc agagcgtgat cgaccgcctc aactggatgc
     3241 gcgatgtgca ggggccaatg ctgcgcgacg cgatgaaaat tatcggtgag atcgatctgc
     3301 gcttaatgct ggcgcaggcg ctgcatatgg gcgatgagtg ccataaccgc aataacgccg
     3361 ggacgacgct gctgattcag gcactgacgc cggggatcat tcaggcaggc tattccgtcg
     3421 agcaacagcg cgaagtgttc gagtttgtcg ccagcagcga ctacttctcc ggcccgacgt
     3481 ggatggcgat gtgtaaagcg gcgatggatg cggcgcacgg catcgaatac agcaccgtgg
     3541 tcaccaccat ggcgcgtaac ggcgtcgagt tcggcctgcg ggtcagcggc ctgccggggc
     3601 aatggtttac cggcccggcg cagcaggtga tcggcccgat gtttgccggt tataagccgg
     3661 aagattcggg gctggatatc ggcgacagcg ccatcaccga aacctacggt attggtggat
     3721 ttgctatggc gaccgcgcct gcaatcgtcg cgctggtggg cggcacggtg gaagaggcta
     3781 tcgatttctc ccgtcagatg cgtgaaatca ctctcggtga aaaccccaac gtcaccattc
     3841 cgctgctcgg ttttatgggc gtgccgtcgg caatcgacat cacccgcgtg ggtagcagcg
     3901 gcattctgcc ggtgatcaac accgccatcg cccataaaga tgcgggcgtc ggcatgattg
     3961 gcgcgggcat tgtgcatcca ccattcgctt gcttcgagaa agccattctc ggctggtgcg
     4021 aacgttacgg cgtctgactt acatgtttat gccggatgcg gcgtgaacgc cttatccggc
     4081 ctacggatgg cccgagaatt gtaggcctga taagcgtagc gcatcaggca gtctggcgtt
     4141 ggtcataacc tcatcaccct ctgtagcgga cataacaaca tgaaagagct tgtggtcgtt
     4201 gccattggtg gcaacagcat tatcaaagat aacgccagcc agtcgattga gcatcaggcg
     4261 gaggcggtga aagccgtggc cgatatggtg ctggaaatgc tggcttccga ttacgacatt
     4321 gtgctgaccc acggtaacgg gccgcaggtc gggctggatt tacgccgtgc ggagattgcc
     4381 cacgagcgcg aagggctgcc cttaacgccg ctggcgaact gtgtggcaga tacgcagggc
     4441 ggcatcggct atctgatcca acaggcgctg aataaccgac tggcgcgtca cggcgagaaa
     4501 aaagccgtca ccgtggtaac tcaggtggaa gtggataaaa acgatccagg ttttgcccat
     4561 cccaccaagc ccattggtgc attcttcagt gaaagccagc gtgacaaact acaaaaggca
     4621 aaccctgact ggtgttttgt tgaagatgcc ggacggggct atcgccgcgt ggtcgcctcg
     4681 ccggaaccga aacgtattgt cgaagcaccc gccattaagg cgctgatcca acaaggtttt
     4741 gtcgtaattg gcgcgggcgg cggtggaatt ccggtagtgc gtactgaagc gggggattac
     4801 caaagcgtgg acgcggttat tgacaaagat ctctctaccg cgctgctggc ccgtgaaatt
     4861 cacgccgaca ttctggtgat caccaccgac gtcgaaaaag tctgtatcca ctttggtaaa
     4921 ccgcagcagc aggcgctgga tcgggtggat attgccacca tgactcgcta tatgcaggaa
     4981 gggcattttt ctcccggcag catgttgcca aaaatcatcg ccagcctgac gttcctggag
     5041 cagggcggca aagaagtgat tatcaccacg ccggaatgcc tgcctgcggc gctgcgcggt
     5101 gaaacgggca cccatattat taaaacgtaa ggacgtagga tgaaagaaag caatagccgc
     5161 cgtgaatttc tgagccagag cggtaagatg gtcaccgccg ccgcgctgtt tggtacctat
     5221 gtgccgctcg cccatgcggc ggtagctggc accctaaact gcgaagcgaa caacaccatg
     5281 aaaatcactg acccgcatta ctatctcgat aacgtgctgc tggaaaccgg ttttgactac
     5341 gaaaacggtg tggcggtgca aacccgcacg gcgcgccaga ccgtggagat tcaggacggc
     5401 aaaattgtcg ccctgcgcga gaacaagcag catccggacg ccacgctgcc gcactatgac
     5461 gctggcggta agttgatgct acccaccact cgcgacatgc atattcatct cgacaaaacc
     5521 ttctacggcg ggccgtggcg ctcgctcaat cgtccggcag gcaccactat ccaggacatg
     5581 atcaaactca agcagaaaat gctgccggag ttacaaccgt atacgcagga gcgggcggaa
     5641 aaactgatcg atctattgca gtcgaaaggc accaccattg cccgcagcca ctgcaatatc
     5701 gaaccggttt ccggcctaaa aaatctgcaa aatttgcagg cggtgctggc gcggcgtcag
     5761 gcgggcttcg agtgtgaaat cgtcgccttc ccgcagcacg gtttgctgct gtcgaaatcg
     5821 gaacccttaa tgcgcgaagc gatgcaggcg ggtgcgcatt acgtcggcgg gctggacccg
     5881 accagtgtcg atggcgcgat ggaaaaatcc ctcgacacca tgtttcagat tgcgctggac
     5941 tacgacaaag gtgtcgatat tcacctgcac gaaaccactc cgtcgggcgt ggcagccatc
     6001 aattatatgg ttgaaacggt agagaaaacg ccgcaactga aaggtaagct gaccatcagc
     6061 cacgcctttg cgctggctac gctcaacgaa caacaggtcg atgcgctggc gaatcggatg
     6121 gcggcgcagc aaatttctat cgcctcgacg gtgccgattg gcacgctgca tatgccgctc
     6181 aaacagttgc acgacaaagg cgtaaaagtg atgactggca ctgacagcgt tatcgaccac
     6241 tggtcgcctt atggtctggg cgacatgctg gaaaaagcca atctctacgc gcagctctat
     6301 attcgtccta acgaacagaa cctctcccgt tcgctgtttc tagccactgg cgatgtattg
     6361 ccgctgaatg aaaaaggcga gcgtgtatgg ccaaaagcgc aggatgatgc cagctttgtg
     6421 ctggtggacg cctcctgttc cgccgaggcg gtggcgcgta tctcgccgag aaccgcaacg
     6481 ttccataaag ggcaactggt gtgggggagt gtggcaggtt gatgcgcatt tgaaacgcct
     6541 gcaacggtga attgtaagag atttaagccc cagggagtgg ggcgacctca atatgatagt
     6601 gtcggcagaa atggacatta tctttgagga atatgccctt attgacgttg tgtagataac
     6661 tatttgtcta cgtgaaaaga tcatcagttt tgccgatttt agtcttttac ggatagcaaa
     6721 tatcacactt ataggcgctc gccttatggg gaacgttgct gctctgtttc attcaggttc
     6781 gcggcatgtt ggggctggat cgggtggttt aaatattggc tatattcaat ggacgcgttt
     6841 tgccgcgatg acatatcagg cgtcgccaaa tacacatagc taatcaggag taaacacaat
     6901 gaagatcaaa gctgttggtg catattccgc taaacaacca cttgaaccga tggatatcac
     6961 acggcgtgaa ccgggaccga atgatgtcaa aatcgaaatc gcttactgtg gcgtctgcca
     7021 ttccgatctc caccaggtcc gttccgagtg ggcggggacg gtttacccct gcgtaccggg
     7081 tcatgaaatt gtggggcgtg tggtagccgt tggtgatcag gtagaaaaac atgcgccggg
     7141 cgatctggtc ggtgtcggct gcattgtcga cagttgtaaa cattgcgaag agtgtgagga
     7201 cgggctggaa aactactgtg atcacatgat cggcacctat aactcgccga cgccggacga
     7261 accgggccat actctgggcg gctactcaca acagatcgtc gttcatgagc gatatgttct
     7321 gcgtattcgt cacccgcaag agcagctggc ggcggtggct cctttgttgt gtgcagggat
     7381 caccacgtac tcgccgctac gtcactggca ggccgggccg ggtaaaaaag tgggcgtggt
     7441 cggcatcggc ggtctgggac atatggggat taagctggcc cacgcgatgg gggcacatgt
     7501 ggtggcattt accacttctg aggcaaaacg cgaagcggaa aaagccctgg gggccgatga
     7561 agttgttaac tcacgcaatg ccgatgagat ggcggctcat ctgaagagtt tcgatttcat
     7621 tttgaataca gtagctgcgc cacataatct cgacgatttt accaccttgc tgaagcgtga
     7681 tggcaccatg acgctggttg gtgcgcctgc gacaccgcat aaatcgccgg aagttttcaa
     7741 cctgatcatg aaacgccgtg cgatagccga ttctatgatt ggcggcattc cagaaactca
     7801 ggagatgctc gatttttgcg ccgaacatgg catcgtggct gatatagaga tgattcgggc
     7861 cgatcaaatt aatgaagcct atgagcgaat gctgcgtggt gatgtgaaat atcgttttgt
     7921 tatcgataat cgcacactaa cagattgaaa aaattaataa atacgctg
//
LOCUS       DAEEKQ010000174         7948 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.176, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000174 DAEEKQ010000000
VERSION     DAEEKQ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.176"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..497
                     /gene="radC"
                     /locus_tag="KLK57_003550"
     CDS             <1..497
                     /gene="radC"
                     /locus_tag="KLK57_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HBE0893825.1"
                     /translation="LTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            714..950
                     /gene="rpmB"
                     /locus_tag="KLK57_003551"
     CDS             714..950
                     /gene="rpmB"
                     /locus_tag="KLK57_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HBE0893826.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            971..1138
                     /gene="rpmG"
                     /locus_tag="KLK57_003552"
     CDS             971..1138
                     /gene="rpmG"
                     /locus_tag="KLK57_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HBE0893827.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            1236..2045
                     /gene="mutM"
                     /locus_tag="KLK57_003553"
     CDS             1236..2045
                     /gene="mutM"
                     /locus_tag="KLK57_003553"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HBE0893828.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(2084..2563)
                     /gene="coaD"
                     /locus_tag="KLK57_003554"
     CDS             complement(2084..2563)
                     /gene="coaD"
                     /locus_tag="KLK57_003554"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HBE0893829.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALTQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALVAKLA"
     gene            complement(2571..3848)
                     /gene="waaA"
                     /locus_tag="KLK57_003555"
     CDS             complement(2571..3848)
                     /gene="waaA"
                     /locus_tag="KLK57_003555"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HBE0893830.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            4261..5319
                     /gene="rfaQ"
                     /locus_tag="KLK57_003556"
     CDS             4261..5319
                     /gene="rfaQ"
                     /locus_tag="KLK57_003556"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HBE0893831.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTTPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            5316..6440
                     /locus_tag="KLK57_003557"
     CDS             5316..6440
                     /locus_tag="KLK57_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HBE0893832.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYHLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            6433..7230
                     /gene="rfaP"
                     /locus_tag="KLK57_003558"
     CDS             6433..7230
                     /gene="rfaP"
                     /locus_tag="KLK57_003558"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HBE0893833.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGI
                     NPLSRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAVGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFVAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            7246..>7948
                     /locus_tag="KLK57_003559"
     CDS             7246..>7948
                     /locus_tag="KLK57_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide 1,3-galactosyltransferase"
                     /protein_id="HBE0893834.1"
                     /translation="MSAHYFNPQEMINKTIIFDERPAASVASSFHVAYGIDKNFLFGC
                     GVSITSVLLHNNDVSFVFHVFIDDIPEADIQRLAQLAKSYRTCIQIHLVNCERLKALP
                     TTKNWSIAMYFRFVIADYFIDQQDKILYLDADIACQGNLKPLITMDLANNVAAVVTER
                     DANWWSLRGQSLQCNELEKGYFNSGVLLINTLAWAQESVSAKAMSMLADKAVVSRLTY
                     MDQDILNLILSGKVKF"
BASE COUNT         2023 a         1836 c         2031 g         2058 t
ORIGIN      
        1 tgttaacctc tgaatatgaa caatttagtg gcgttcatgg aattggcgta gcgaaatttg
       61 cccagttaaa ggggattgct gaactggcgc ggcgttacta caacgtgcgg atgcgtgaag
      121 aaagcccttt actcagcccg gagatgacgc gggaattttt acaaagccag ctcacgggtg
      181 aggagcggga gatctttatg gtgatctttc tcgactcaca acaccgggtt ataacgcata
      241 gccgtctttt ttccggcacg ctaaaccatg ttgaagtcca tcctcgggaa attatccggg
      301 aagcgataaa aataaacgcc tcggcgctga tccttgcaca taatcaccct tcgggttgtg
      361 ctgaacccag taaagcggat aaactcatta ctgaacggat aataaagagt tgtcagttca
      421 tggatttacg cgtgctcgac catatcgtga ttgggcgtgg agagtatgtt tcttttgccg
      481 aacgcggctg gatttaaccc gctatgcgcg atccttcggg atctttgtct gttcgggact
      541 tgagcacatc gctgagtcag cgtatactac gccacctttg agaatctcgg gtttggcatt
      601 tgggcctggc aatcgagagt tcacatagaa ctgcgatgac cgggctgtaa agcctgacga
      661 ggcgccaata ccccatacga agctcgagct aatttgattt ttggagaata gacatgtccc
      721 gagtctgcca agttactggc aagcgtccgg tgaccggtaa caaccgttcc cacgcactga
      781 acgcgactaa acgccgtttc ctgccgaacc tgcactctca ccgtttctgg gttgagagcg
      841 aaaagcgttt tgtcaccctg cgcgtatctg ctaaaggtat gcgtgtaatc gataaaaaag
      901 gcatcgatac agttctggct gaactgcgtg cccgtggcga aaagtactaa gtacttagag
      961 gaaataaatc atggctaaag gtattcgtga gaaaatcaag ctggtttctt ctgctggtac
     1021 tggtcacttc tataccacta cgaagaacaa acgtactaag ccggaaaaac tggaactgaa
     1081 aaaattcgat ccagttgttc gccagcacgt gatctacaaa gaagcgaaaa tcaaataatt
     1141 ctcgctttga tgtaacaaaa aacctcgctc cggcggggtt ttttgttatc tgcttgcccc
     1201 catattgatt gcatctgttc attcctggag atgctatgcc tgaattaccc gaagttgaaa
     1261 ccagccgtcg cggcatagaa ccgcatctcg ttggtgcaac cattcttcat gcggtggtgc
     1321 gcaacggacg cttgcgctgg ccggtttcag aagagatcta ccgtttaagc gaccaaccag
     1381 tgcttagcgt gcagcggcgg gctaaatatc tgctgctgga gctgcctgag ggctggatta
     1441 tcattcattt ggggatgtct ggcagcctgc gcatccttcc agaagaactt ccccctgaaa
     1501 agcatgacca tgtggatttg gtgatgagca acggcaaagt gctgcgctac accgatccgc
     1561 gccgctttgg tgcctggctg tggaccaaag agctggaagg gcacaacgtg ctggctcatc
     1621 ttggaccgga gccgcttagc gacgatttca atggtgagta tctgcatcag aagtgcgcga
     1681 agaaaaaaac ggcgattaaa ccgtggctga tggataacaa gctggtggta ggggtaggga
     1741 atatctatgc cagcgaatca ctgtttgcgg cggggatcca tccggatcgg ctggcgtcat
     1801 cactgtcgct ggcagagtgt gaattgttag ctcgggtgat taaagcggtg ttgctgcgtt
     1861 cgattgagca gggtggtaca acgctgaaag attttctgca aagtgatggt aaaccgggct
     1921 atttcgcgca ggaattgcag gtttacggac gaaaaggtga gccgtgtcgg gtgtgcggta
     1981 cgccgattgt ggcgactaaa catgcgcagc gggcaacgtt ttattgtcgg cagtgccaga
     2041 agtaattcat acgcgccgga tggcatacca tccggcataa acgctacgct aacttcgcca
     2101 ccagcgcctg atggacattc tccggcagga aatgggtgac atcgccctga tggcgcgcca
     2161 cctctttcac caacgatgaa gagataaacg accactcttt cgacggcatc agaaacacac
     2221 tttccagttc cggcattaag tggcgattca tatgcgccag ctgcatttca tattcaaaat
     2281 ccgccaccgc acgcaggcca cgaatcagca ccgtagcgtg ttgattacgg gcgaagttcg
     2341 ccatcaaatc actaaaccca accacttcca cgttccccag atgcgcggtt gcctgctgtg
     2401 tcagcgccac acgctcttcc agggtaaaca tcggtttttt actggggctg gcggcaatcg
     2461 ccagaataac gtgatcgaac atttgcgtgg cgcgcgtcac gatatcgata tgaccattgg
     2521 taatgggatc gaaagtaccc ggataaatcg cccgtttttg cataacaacc tcaatgcgtt
     2581 ttcggtggca ggtaaggttc cagcagttga agcagacgct gtagcgcgcc ctggttttga
     2641 tacagtactt caacggcatg acggccatag aaactacggt aatcggcgtc ggtgagtaaa
     2701 gaggaaacct cttttgcaag cgtagtggca tcggtaacgg taatcagccc gcttgcctgc
     2761 tccagccgcg cgcaaatgtc tttgaaatta aaagtatgcg gccccatcaa taccggaata
     2821 gcgtgtgcgg cagcttccag aggattatgc cctccacgtt caaccagtga accgccaaca
     2881 aaggcgagat cggcaatgcc gtacagtaac atcaactcgc ccatcgtatc gccaaccaca
     2941 acctgcgtgc tggtggaggg gacttcccct gaagagcgtg tgatatagct tagtccagcc
     3001 tggcggacaa ggttaatcgc atccgggaag cgttctggat gacggggtac caggatgagc
     3061 aataaattcg ggaattgctg taataatgcc tgatgtgcgg cgatcaccac actctcttca
     3121 ccttcgtgag tgctggtggc aatccatacc gggcggtgtg gtgcccactg gcggcgcagc
     3181 gtcacggctt tagcagccaa ctgcggcgtt acagaaatat cgaatttcag gctaccggta
     3241 acggttacct ggttattttt tgcgcccagc gccacaaaac gtgcaccatc ttcttcattt
     3301 tgcgcagcaa tcagcgtaat acgacgcagt aagcggcgga cgaatttacc cagtttggca
     3361 taacctgcgg ccgagcgggc agagagtcgc gcgttagcga tcaccagcgg aattttacgt
     3421 ttatgtagcg ccgcaatcag gttaggccat agttcggttt ccataatcaa caccagtttg
     3481 gggtcgactt tattcaggaa acggttgagc gcatcgggca ggtcatacgg cagataaacg
     3541 tgctgaacat ccttcccgaa agccgattgt acgcgctccg aaccggttgg cgtcatggtt
     3601 gtaacggtaa tcggtaaatc aggataacga tggcgcagcg cgcgcaccaa cgggatcgcc
     3661 gccagagttt caccgacgga gacggagtgc agcataatgc cgcctggttt tagcggatgg
     3721 cggtaaaaac cgtaacgttc accccagcgt tttcgatagg ccggagcctt acgtccgcgc
     3781 acccagagcc gtatccagat cagcggctga ataaggtaga gaagggcggt gtaaagcaat
     3841 tcgagcatag taaatagctg acttatggat gtgctgggga ttctatgtat ttagctgtgg
     3901 ctttaccatt acttttcccg tttttgactt aaatagcaat ttacccgcca attcttgctg
     3961 aacatctata ctttttttgt atttttatgc gattcattga tttctgctgt acgtgcagga
     4021 aggaatcgca gagcaacagt gaaagaatat ggttgctaat gattttattt gattgatttc
     4081 tttattgaaa atcaacaatg tgcttaaaaa gcattcgggt cggattgccg cgaaagaagt
     4141 gatacactag cgcgtcaata tcagtgcggt atcagtattc aggtagctgt tgagcctggg
     4201 gcggtagcgt gctttttctg cttcacctta atcggataat ctcaacaaaa agagttactt
     4261 gtggataagc catttcgaag aattttgctc attaagatgc gttttcatgg ggatatgtta
     4321 ttaactactc ccgtcattag ttcgctgaaa aaaaattacc ctgacgcaaa aatcgatgtg
     4381 ctgctttatc aggacaccac cccgatcctg tctgaaaatc cagagattaa cgcgctctac
     4441 ggcataaaaa ataaaaaagc aaaagcctca gaaaaaattg ccaacttttt tcatctcatc
     4501 aaggtattac gtgccaataa gtatgacctt atcgtcaatc ttaccgatca atggatggtt
     4561 gctatactgg ttcgcttatt aaatgcccgt gtgaaaattt cccaggatta tcatcatcgg
     4621 cagtctgctt tttggcgtaa aagtttcacc catttagtgc cgttgcaggg tggaaatgtg
     4681 gtggaaagta acttatccgt gctgacacca ttgggacttg attcgttggt gaagcagaca
     4741 accatgagtt acccgcctgc aagctggaaa cgtatgcgtc gcgaacttga tcacgctggt
     4801 gttggacaaa attatgtggt tatccaacct acggcgcggc aaatcttcaa atgctgggac
     4861 aacgccaagt tttccgctgt gattgatgcc ttacatgctc gtggttatga agtcgttctg
     4921 acgtccggcc cagataaaga cgatctggcc tgcgtcaatg aaattgcgca gggatgccag
     4981 acgccaccag taacggcgct ggctggaaag gtgaccttcc cggaacttgg tgcgttaatc
     5041 gatcatgcgc agctgtttat tggcgttgat tccgcaccgg cgcatattgc cgctgcagtt
     5101 aatacgccgc tgatatcgct gtttggtgcg acagaccata ttttctggcg tccttggtca
     5161 aataacatga ttcaattctg ggcgggagat taccgggaaa tgccaacgcg cgatcagcgt
     5221 gaccgaaatg agatgtatct ttcggttatt ccggcggcag atgtcattgc tgctgtcgat
     5281 aaattactgc cctcctccac gacaggtacg tcgttatgat cgttgctttt tgtttatata
     5341 aatattttcc ctttggcggt ttgcagcgcg attttatgcg tattgctcag acagtcgccg
     5401 cccgaggtca tcatgttcgg gtttatactc agtcgtggga aggcgaatgc cctgatgtat
     5461 ttgaactgat caaagtgccg gttaaatcgc ataccaatca cgggcgcaat gcggagtatt
     5521 ttgcctgggt gcaaaaacat ttacgcgaac atcccgtcga taaagtcgtt ggattcaaca
     5581 aaatgccggg gctggacgtt tattatgccg ctgatgtttg ttatgccgag aaagtcgcgc
     5641 aggaaaaagg ctttttctat cacctgacgt cacgttatcg ccattatgcc gcctttgagc
     5701 gggcaacctt cgaacagggc aagccgacac agctgctgat gctgacagat aagcaaatcg
     5761 ccgatttcca gaaacattat cagactgaag cggagcgttt tcatattctg ccaccgggga
     5821 tttatcctga tcgtaaatat agccagcagc cagctaatag ccgtgaaatc ttccgtaaga
     5881 agaatggaat aaccgaacaa caatatttat tgttgcaggt cggttcagac ttcacgcgta
     5941 aaggtgtcga tcgttccatt gaagcacttg cttcgttacc ggattcgctg cgccacaaca
     6001 cattgctata tgttgttgga caggataagc cgcgaaaatt tgaggcgctg gcagaaaaac
     6061 gcggcgtgcg cagtaatgtt cacttcttct cggggcgcaa cgatgtctcg gaattaatgg
     6121 cggcggcgga tttattactg catcctgcct accaggaagc agcgggaatt gtgctactgg
     6181 aagcgattac tgcaggatta ccggtactaa caacagccgt ttgtggctat gcgcattata
     6241 ttgtcgacgc caattgcggc gaggctattg ctgagccatt ccgccaggaa acattgaatg
     6301 agattttacg caaagcgtta acgcaatctt cattgcgcca ggcctgggcg gaaaatgcgc
     6361 gacattatgc tgatacacaa gatttataca gtctgccaga gaaagcggcg gatatcataa
     6421 cgggtggtct ggatggttga acttaaagag ccgtttgcca cgttatggcg cggtaaagat
     6481 ccttttgagg aagttaaaac cttgcagggt gaggtttttc gtgaactgga aactcgccgc
     6541 actctgcgat ttgaaatggc gggcaaaagc tattttctca aatggcatcg cggcacgacc
     6601 ctgaaagaga taatcaaaaa tttactctca ttgcggatgc cagtattagg cgcagaccgc
     6661 gaatggaatg cgattcatcg actgcgggat gtcggcgttg atactatgta tggggtggca
     6721 tttggcgaaa aaggtattaa tccgctgagc agaacctcgt ttattattac cgaagatctg
     6781 accccaacca tcagtctgga agattactgt gctgactggg cgaccaaccc accagatgtt
     6841 cgcgtaaagc gtatgcttat taagcgtgtc gcgacgatgg tgcgcgatat gcatgctgtg
     6901 ggcattaacc accgtgactg ttatatctgt catttcctgc tgcacttgcc tttttccggt
     6961 aaggaagagg agttaaaaat ttcggtaatt gacctgcacc gggcgcagct tcgcacgcgc
     7021 gttccacgtc gttggcgcga taaagatctt attgggcttt atttttcttc gatgaatatc
     7081 ggcctgactc agcgggatat ctggcggttt atgaaagtgt attttgtcgc cccgcttaaa
     7141 gacattctca agcaggaaca aggactgctg tcgcaagcag aagcaaaagc cacaaaaatc
     7201 agggaaagaa cgattcgaaa atcgttgtaa tttccggaga gaaatatgag tgcccactat
     7261 tttaatccac aagagatgat caataagaca atcatcttcg atgaaaggcc agcggcgtca
     7321 gtggcatcat cattccatgt tgcttatggc attgataaaa actttctttt tggttgtggt
     7381 gtttcaatca cgtcagtttt gttacataac aacgacgtga gttttgtttt ccacgttttt
     7441 attgatgata tccctgaagc cgatatccag cgtttagccc aattggcgaa aagctatcgt
     7501 acctgtatcc agatccatct ggtaaattgt gaacggctta aggcattacc gacgaccaaa
     7561 aattggtcta ttgccatgta tttccgtttt gtaattgcag attactttat tgatcaacaa
     7621 gataagatcc tttacctgga tgctgatatc gcctgtcagg gaaacttaaa gccgctgata
     7681 acaatggatc ttgccaataa cgttgctgct gttgttactg aacgcgatgc taactggtgg
     7741 tcgttacggg gtcaaagtct gcagtgtaat gaacttgaaa agggttactt taattcaggt
     7801 gtcctgttaa ttaatacact agcgtgggcg caggagtccg tttctgctaa agcgatgtcg
     7861 atgcttgctg ataaagccgt cgtttcccgt ttaacctata tggatcaaga tatacttaat
     7921 cttatcctgt cagggaaagt taaattca
//
LOCUS       DAEEKQ010000175         7931 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.177, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000175 DAEEKQ010000000
VERSION     DAEEKQ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.177"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            812..1054
                     /locus_tag="KLK57_003560"
     CDS             812..1054
                     /locus_tag="KLK57_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893835.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="KLK57_003561"
     CDS             complement(1384..2313)
                     /gene="fdhE"
                     /locus_tag="KLK57_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HBE0893836.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="KLK57_003562"
     CDS             complement(2310..2945)
                     /gene="fdoI"
                     /locus_tag="KLK57_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HBE0893837.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="KLK57_003563"
     CDS             complement(2942..3844)
                     /gene="fdoH"
                     /locus_tag="KLK57_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HBE0893838.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="KLK57_003564"
     CDS             complement(3857..6907)
                     /gene="fdnG"
                     /locus_tag="KLK57_003564"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6320..6322),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HBE0893839.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            7101..>7931
                     /gene="fdhD"
                     /locus_tag="KLK57_003565"
     CDS             7101..>7931
                     /gene="fdhD"
                     /locus_tag="KLK57_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HBE0893840.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         1831 a         2054 c         2072 g         1974 t
ORIGIN      
        1 atttacatca ttccctgtct atgccggtaa tattgaataa gaactctata aatttttatt
       61 cttcgttggc accatacgtg tcttgctaca tcactgttca ggagcattta aatattattt
      121 gtagagctga gtggcaatta tagcagataa tgcttaactc atcatcttag ttcaggtttg
      181 agcgattttt gatagtcaaa acctcctctg gtgccatact tttctcataa atatttttaa
      241 aatctatagc tcacaaacat tatgcaaaga taatgggctg gcatcattgt cagtatcatt
      301 catttactgc tctgtatgtt atgaatcatt atgttgtatg tttgctctta aaccgtggga
      361 aggaggctat aatggttgcc aatattaatc tcattaaaca agaaagttat tctgttgtta
      421 atttagaaaa acaactttcc cgaactgtta ccaacaaaat tataacgatg agcaagtaaa
      481 gaccggttgg tggcagtact gaccggtctg tgatgaaaga ttaatttcac cttaaatcct
      541 ggtgctgtta ggatgctgac gtttttcggc tacaccattc agattatgca aataaatatc
      601 cctgaatata atatattcat agcacccata actcatcctg ggcgataact aaaccatttt
      661 tgagtaataa atgaaacaac tgcaacgctc ttattatttc tggaaaacgc ctcgcaaaaa
      721 taaaaaatga tgcgtaaatg agctttcttt aaagattcgg caagggtact tttctctcca
      781 tagattcaaa aatgatattg gcgagatatt tatgaactct ctggcaggta tagatatggg
      841 cagaatttta ctcgatttat cgaatgaggt gattaagcaa cttgatgatc tcgaggtgca
      901 gcgtaatctt cctcgtgcgg acctattaag ggaagcagta gatcaatacc tgataaatca
      961 atcgcaaaca gcaagaacca gtgttcctgg catctggcaa gggtgtgagg aagatggtgt
     1021 cgaatatcag cgtaagctgc gcgaggaatg gtaatgggaa aaaatgatgt caatcagatt
     1081 gctgacaacg tgcgcgttgt tcatgccgga tgcggcgtga acgccttatc cggcctacaa
     1141 agtcatgcaa attcaatata ttgaagttta ttggtaggcc tgataagcgc agcgcatcag
     1201 gcaatcatac gcttgtcatc agtgacatac ccataacctt atcgatgtgt tggagggaag
     1261 aacgaggcaa acagacaatt gcggcatgat cttgaatggg taacgcggaa tacgagagat
     1321 ggtgtcgtca caccctatga gctgtaatcc gggcgacctg cgccacccgg aaaacgtcag
     1381 agattactct ccttcacccg gaaacaggaa cgggttaatg gaactacggg cgtagccttc
     1441 ttgctccatt cgcgcgtcca gtaccagaga ggcgaggtca tctgccacgg cttcaacttt
     1501 cggttctttt tcctgataaa gaatcttcag gtaagtgccg cagtcatcgc agctttcggc
     1561 tttaatcgcg gcctgttcgt catccagcga ccagtaatgc agtttgccgc tctgctcgca
     1621 gttgctgcat tttacgcgca ctacgtgcca ttcggtttca cacaggttgc agtgcaggta
     1681 acgcagaccc tgagtggtgc caatttgcac catgctggac accggcatag agccacatac
     1741 cgggcaatat tgacgttgtt cgccgtattc agcgcgggct ttgccgggga tcagattggc
     1801 catctgcgcc cagtagagcg acagcgcagc ccagataaac ggcgctttat cgctgctgac
     1861 ggacgagaaa tcagaggcaa acagcgcgct ggccatatct tccagctcct gagtcgatgc
     1921 cttctccaga ttctcaatca ctgccagcgc cgggccgctc atttcaggtt tcagctcagc
     1981 aattagcgcc atcagcagct tttgccagtg cttatcacgc ggcagaacgt gaatatccag
     2041 cgggggcttg ccctgtgcgc tggcttcttt aatgcgcgcg gtcagatcca tctccagcgg
     2101 atggtcgtac agcaccactt cctgggcgtg ggcgataagc gcagcaaagc gcaggtaatc
     2161 acccagcgga ttattttctg ccagctcgcg cagacgctcg gcgcggcggt tgtataaatt
     2221 cttgagccga gggaacaata acggcggaat catatccgcc gtacgtttct cgctcgaacc
     2281 cagctcatct tgcgggatta tgcgaatact cattcagctt tcttttccgt tgtcttgcgg
     2341 acctcacggt accagcgcgg gtgatgtttc ttcgcccagg cgctggtaac ccatccttcc
     2401 accatggcgg taatcgtgcc tttcacccaa agggcggcgt agatatgcac catgataacc
     2461 acaattaacg ccactgcggc aaatgaatgc agcattaacg cgaatcggat caccgggatt
     2521 gagaaagcag gcgcaaaata aggacgccag ataatcacgc cgctcaccag cagcagaacc
     2581 aggaaaataa tcgccgccca gaaaacgcat ttctgaccga agttataacg cccggtgtca
     2641 cctacttcct cgttgacgac gatcttacga atattcttcg cccaaaagat atcatcccga
     2701 ttgattaggt tgtgatgcca gtaacggaaa aacatgatga tgaacgaggc aaacataacc
     2761 acgccgacaa acgggtgcag aattcgcgcc agctgcggtg tgcccatgat ttgcatcaac
     2821 cagttgaagg acgggaacaa aaagcccagc ccgctcaccg ccgccaggat gaagcagaag
     2881 gcggtgatcc agtggttgat acgttccggc gcggtgtagc gcacgatggt gtcacgtcgt
     2941 ttcatttgcg ctcctcgtct ttctcttcgt gcagattatt ctcttcctca tccgcacggt
     3001 tcggaccgac acccacgtag tggaagatac tggccgcaaa ggtagccgca aagccaacag
     3061 ccgcgagcgg tttccagatg cctttccaga atttcacggt ttcgctgatt tccgggttct
     3121 ccggcaagcc atgatacaga tttggcttgt cagcatggtg cagcacgtac atgacgtgtg
     3181 taccaccgac gcctgccgga tcgtacagac ccgcattgtc gtaaccgcgg gttttcagct
     3241 cagcaacgcg ctcgctcgcc agcgttttca tcgactcttt cgtaccgaag tgaatcgcgc
     3301 ccgttgggca ggtcttcacg caggctggtt cttgcccaac caccacgcgg tcaacgcaca
     3361 gcgtacattt gtagacgcgg ttgtcttccg ggttgaggcg cggaatgtcg aacggacagc
     3421 ccgcaatgca ataaccgcag ccaatgcact gctcggactg gaagtcgacg ataccgttgg
     3481 catactgaat aattgcacct tccgccgggc acgctttcag gcagcctgga tcggaacagt
     3541 gcatacagcc gtctttgcgg atcagccatt ccagtttgtc gttctgctcc acttccgaga
     3601 agcgcatcac cgtccacgat ttggcgctta aatcattggg gttgtcgtac accccaatgt
     3661 tattgccgac ggtatcgcga atgtcgttcc actctgaaca cgccacctga caggctttac
     3721 agccgataca ggtggtgacg tcgatgagtt tcgccacttc ttcctggaag tcccgcgcct
     3781 gaggcgcggg ggtcagaccg ttagtcgcgg aacgacgaat gatatcttgc gattgataag
     3841 ccatatgtcg tctccgttac accttttcca cattcacaag gaaggactta aactccggcg
     3901 tctgcgtgtt cgcatcacca acgaatggcg ttaacgtatt ggcaataaag ccttttttag
     3961 ctacgccttc atagccccag tgaatcggaa taccgatggt atcgatatct ttgccgttcg
     4021 ctttcagcgt gcgaatacgt ttggtcacca ccgctttggc tttgatatag ccacggttgg
     4081 aggagacttt cacggtatcg ccctgggcaa tgccaagttt attcgccagc gactccccga
     4141 tttccacaaa ctgctctggt tgcaaaatcg cgttcaacag cgcgtgtttg gtccagtagt
     4201 ggaagtgctc ggtcagacga taggtggttc cgacatacgg gaacttatcg gctttaccca
     4261 atgcttcggc gtcgtcttta aagattcgcg cagccgggtt cgagataacg tttggatgca
     4321 gcgggttagt tcccagcggc gtttcaaacg gctcgtagtg ttccgggaac ggaccttccg
     4381 ccatcttatc gagggcaaac agacgcccca tgccttcctg ctgcatgata aacggcccga
     4441 cgccgctgcc cggaggcgct gcgctgtagt ccggaatatc ccagccggtc cacttagtgc
     4501 cgtcccattt cagcaactga cgcttcggat cccacgggtt accctgcgga tctgcggagg
     4561 cgcggttata cagaatgcgg cggttaagcg gccatgccca tgcccagccc agcgtgttac
     4621 cgaggccaga cggatcggcg ttatcacgac gcgccatctg gttgccttcc ggcgtccagc
     4681 taccggcgaa aatccagcag ccacaggagg ttgtaccgtc atcgcgcaat tgggcgaacg
     4741 agctaagttg ttggcctttc ttgacgataa ccgcaccggt tgccggatcg gtaatatcgg
     4801 ccagcgcctt accgttgctc tccatcgcca cttcttccga cttcggctca tgcggaatgg
     4861 cgtagttcca ggtcatgttc agcacctgat ccgggtttgc gccaccctgt tcggcataca
     4921 tcttgcgcaa gcgcaggaag ataccggaga ggatctcgcc atcagtcagc gcaatccctg
     4981 gggcgtccgc acctttccag tgccactgca accagcgacc tgagttaacg attgaaccgt
     5041 tctcttctgc gaagcaggtc gatggcagac ggaacacttc ggtctggatc ttcgacgagt
     5101 caacttcgtt cagctcaccg tggttctgcc agaagttaga ggtttcagtg ttcagcgggt
     5161 cgatggtgac gaggaacttc agtttcgaca gacaaccgat cactttgttt ttgttcggga
     5221 atgaggcaac agggttaaag ccctggcaga tatagccatt gaccttgccc tctttcatca
     5281 tctcgaagta ttgcaggacg tcgtagcctt tatcccactt cggcaaccag tcaaagcccc
     5341 agctattttc cgccgtcgct ttatcaccaa agaaggcctt catcatagag acgaagaatt
     5401 tcgggtagtt gccccagtag tttacctggc cttccagcag cggttttggc gtgttggcgg
     5461 taaggtaggt ttgcagatcg gtctgcttct cgcttggcag cgtcatgtaa cctggcaggc
     5521 tctgcgacag cagccccagg tccgtcagcc cctgaatatt ggagtgaccg cgcagggcgt
     5581 taacgccgcc gcctgccatc cccatattgc cgagcagcag ctggatcatc gccatcgtac
     5641 gaatgttttg cgcaccaacg gagtgttgcg tccagccgag ggcatacagg aacgaggcag
     5701 ttttatcgtg agcactggtt tctgcgatgt attcgcagac tttcaggaac gcgtcttttg
     5761 gcgtaccaca gatgttttca accacatctg gcgtgtagcg ggaaacgtgc tgtttcagca
     5821 agttccacac gcagcgcgga tgttgcagcg tggtatcgcg tttggcgaag ccgttttcgt
     5881 ccagttcgta agtccaggag gatttatcgt acttgcgttt ttccgcgtca tagccggtga
     5941 acaggccatc ttcaaagccg taatcctcac gcacgatcag gctggcgttg gtataggctt
     6001 cggtgtattc gcggttgaat ttttcattgt tcagcaggta cagcaatacg cctgacagga
     6061 aagcaatgtc agtaccggaa cgaatagggg catagtagtc agccaccgcc gccgtacgcg
     6121 taaagcgagg atcgatcaca atcagcttcg cgccattgtg aattttggct tccatcgccc
     6181 agcggaaccc gaccgggtga gcttcagcgg cgttaccgcc catcaccacg acgaggttgg
     6241 cgttcttgat gtcgacccag tggttggtca tcgcaccgcg accaaatgtt ggagcaagac
     6301 ttgctaccgt tggtccgtgt cagacacgcg cctggttgtc gaccgcgagc atacccagcg
     6361 cgcgggagaa tttttgcgtt aaatagccgg tttcgttact cgacgcggaa gcacacagca
     6421 tcccggtgga gagccagcgg ttaacagtca cgccttcggc gttttgcgca atgtagttag
     6481 catcgcggtc ttctttcatc agtttggcga tgcgatcaaa cgcctcttcc caactgattt
     6541 gctgccattt atcagaacct ggcgcacggt attccggaaa cttcagacgg ctttcggagt
     6601 ggatgaaatc caccaggcca gcgcctttcg gacaaagtgc accgcggttg accgggtgat
     6661 ccggatcgcc ttcgatatgg aagatagatg ctttggcgtt ttttgctccg tcaccgaggc
     6721 tgtacatcaa cagcccacag cctacggaac aataggtgca ggtattacgg gtttcgcggg
     6781 tgcgcagcag tttatactgc cgggtttccg cgagtgctac gctgggtgca aaacccagtg
     6841 ccgctgccgt ggtgcctgcc ataccgccag cgcagatctt aaagaactgc cttctgctga
     6901 cctgcatgga ttgctccttg tttcgacatt gtcacgtccc atttacattc gcttgctgcg
     6961 tgtgcaggga gtgggagtta tttttctttg cggaaggggc cgcaaaggtc cagaattggc
     7021 tcaatttccc tccatccagg aagggtttgt aacagaatac cataatgttg gtgtgtgtgt
     7081 tcttatctgg ttaagagaaa gtgaaaaaaa cacagcgaaa agaaatcgaa aatgtgacaa
     7141 atatcacagg tgttcgtcaa attgagttat ggcggcgtga tgatttacaa cacccacggt
     7201 tagatgaggt cgcggaagaa gttcccgttg cgctggtcta caacggcatt tcgcatgtgg
     7261 tgatgatggc gtcgcccaaa gaccttgagt actttgcgct cggtttttcg ctttccgagg
     7321 ggataataga aagtccgcgc gatatcttcg gcatggatgt cgttccttcc tgtaatggtc
     7381 ttgaagtaca aattgagctt tccagccgcc gctttatggg gttgaaggag cgccgccggg
     7441 cgctggcggg acgtacgggc tgtggcgtat gcggcgtgga gcaacttaat gacatcggaa
     7501 aaccggtgca gccgctaccg ttcacccaga cgtttgatct caacaaactg gatgatgcat
     7561 tacgtcatct caacgatttt cagccagtgg ggcaactgac tggttgtact cacgccgctg
     7621 cctggatgtt gccatctggc gaactggtcg gcgggcatga agacgtgggt cgccatgtgg
     7681 cgctggacaa actgttaggc cgccggtcac aagaagggga aagctggcag caaggtgcgg
     7741 tactggtttc cagtcgtgcc agttatgaaa tggtgcaaaa gtcggcgatg tgcggcgtag
     7801 agattttgtt tgcggtgtct gccgcgacca cgcttgctgt agaagtggct gagcgctgta
     7861 atctgacact ggtaggtttt tgtaaaccgg gtcgggcaac ggtttatacc catccgcagc
     7921 gtttgagcaa t
//
LOCUS       DAEEKQ010000176         7910 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.178, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000176 DAEEKQ010000000
VERSION     DAEEKQ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7910
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.178"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            78..2393
                     /gene="bisC"
                     /locus_tag="KLK57_003566"
     CDS             78..2393
                     /gene="bisC"
                     /locus_tag="KLK57_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HBE0893841.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYTGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLARCTTGYA
                     VFASYLLGESDGIAKTAEWAAEICGVGAAKIRQLAAIFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFTRRSAVLSSMQGSLPGGCDAVDKIPVARI
                     VEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPELVVISEC
                     FWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDFDVFAEL
                     SERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQLIEMPEN
                     PDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEPDEWQGN
                     AEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITEGDMVRV
                     WNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDLPSSRLG
                     NGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(2362..2802)
                     /locus_tag="KLK57_003567"
     CDS             complement(2362..2802)
                     /locus_tag="KLK57_003567"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE0893842.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(2799..3362)
                     /gene="tag"
                     /locus_tag="KLK57_003568"
     CDS             complement(2799..3362)
                     /gene="tag"
                     /locus_tag="KLK57_003568"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HBE0893843.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            3520..4218
                     /locus_tag="KLK57_003569"
     CDS             3520..4218
                     /locus_tag="KLK57_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0893844.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPNYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTQTDPLWHASVSTLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            4447..5655
                     /locus_tag="KLK57_003570"
     CDS             4447..5655
                     /locus_tag="KLK57_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0893845.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFCVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            5979..7670
                     /gene="eptB"
                     /locus_tag="KLK57_003571"
     CDS             5979..7670
                     /gene="eptB"
                     /locus_tag="KLK57_003571"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HBE0893846.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDETYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKEAAAN"
     gene            7762..7838
                     /locus_tag="KLK57_003572"
     tRNA            7762..7838
                     /locus_tag="KLK57_003572"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7796..7798,aa:Pro,seq:cgg)
BASE COUNT         1815 a         1943 c         2098 g         2054 t
ORIGIN      
        1 ttattcctgg gaatactcgg aaatttgtaa gtaatattta actgctcaat acatctaacc
       61 tttcaggagt cttcggtttg gccaactcat cctcacgata ttccgttctg actgccgccc
      121 actgggggcc catgctggtt gaaaccgacg gcgaaaccgt gtttagctcg cgtggcgcgt
      181 tagccacagg aatggaaaac tccttgcaga gcgcggttcg cgaccaggtt cacagcaata
      241 cgcgggtacg atttccaatg gtgcgcaaag gctttctcgc gtcaccggaa aacccgcaag
      301 gcattcgtgg gcaggatgaa tttgttcgcg tgagttggga tgaggcgctg gatcttattc
      361 accaacaaca taaacgcatt cgtgaggctt atggtccggc atcgattttt gctggttcct
      421 acggctggcg ttcaaacggc gtgctgcata aggcctcgac attattacaa cgctatatgg
      481 cgctggcagg cggttatacc gggcatctgg gggattattc gaccggtgcg gcacaggcga
      541 tcatgccgta tgtcgtaggt ggcagcgaag tttatcaaca gcaaaccagt tggccgctgg
      601 tgctggaaca tagcgatgtc gtggtgttgt ggagtgctaa cccactcaat acgctgaaaa
      661 ttgcgtggaa tgcatccgat gagcaggggc tttcttactt ttccgcactg cgtgacagtg
      721 ggaaaaagct gatctgcatt gatccaatgc gatcggaaac cgtcgatttc tttggcgata
      781 aaatggagtg ggtggcaccg cacatgggca ccgatgttgc gctgatgctg gggatcgccc
      841 atacgctggt ggaaaatggt tggcacgacg aagcgtttct ggcgcgttgc accacaggtt
      901 atgccgtctt cgcctcttat ttgctgggcg agagtgacgg aatagcgaaa accgccgaat
      961 gggcagcaga gatttgtggt gttggcgcag cgaaaatccg ccagctggcg gctattttcc
     1021 accaaaatac caccatgctg atggcaggct ggggaatgca gcgccaacag tttggtgagc
     1081 aaaaacactg gatgatcgtc acgctggcag caatgttggg gcaaatcggc acacccggcg
     1141 gcggttttgg tctttcttac cattttacgc ggcgttctgc ggtgctctct tccatgcagg
     1201 gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc attgttgaag
     1261 cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat ttcccggata
     1321 ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat accaatcgtc
     1381 tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc tggacggcgg
     1441 cggcgaaaca cgcggatatc gttctgcctg cgactacctc ttttgagcgt aatgatctca
     1501 ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg gtgccgccac
     1561 gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt tgggagaagg
     1621 gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa acgttttata
     1681 acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt gctgagttct
     1741 ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg tttattcgct
     1801 tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc ggcaagattg
     1861 aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg catccaatgt
     1921 ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag gtactttctg
     1981 cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc gaattgtacg
     2041 cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg cgcggcataa
     2101 ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc ggagcggtca
     2161 ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg ccggatctgg
     2221 atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc aaagatctcc
     2281 ccagctcgcg gctggggaat ggctgtgcgg gtaatacggc gctggcatgg ctggaaaaat
     2341 acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca taatccaggt
     2401 gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct gcgcacggta
     2461 aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg gatagcgctg
     2521 ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg gtgcgacaaa
     2581 cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct taccgtcctc
     2641 ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg gaatgcagtc
     2701 acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca gccacagttc
     2761 gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt tcccggatag
     2821 caacaacagc caaccacatg atcattcacc agcccacatg cctgcataaa ggagtaacag
     2881 attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ctttagatag ggcgtcggag
     2941 gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg tggctgatga
     3001 tttacaaacg accagacaaa gtcggcaaac ggttcgccgt tctgttccat ttgcaggtac
     3061 gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat cccggcgtcc
     3121 tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg atcgaactga
     3181 tgaaagcagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga taacccagcc
     3241 tgctgccctt caaggcagat catttcgaac agttttttac tgtcagtttc aggcacgccc
     3301 cattcattat catggtaggc aatataaagc gggtcctgac tcacccagcc gcaacgttcc
     3361 atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct tgacgcatcg
     3421 gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat acaatgacaa
     3481 taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa aaaaagcggt
     3541 ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt tctcaacaca
     3601 gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc cacagagcgt
     3661 tacacctggg atagtgatca tcaacctaat tacaacgata ttttgtcgca acgtattcaa
     3721 agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc tgtggatgtg
     3781 accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac aaccggcccg
     3841 gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt tggcgacagt
     3901 actaccacgc agaccgatcc gttatggcat gccagcgtga gtaccttagg ctggcgtgtt
     3961 gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca gcaatttggc
     4021 gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa ccagaacggc
     4081 aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat tgccgcctat
     4141 gccgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta tacgatgggg
     4201 gttagcgcca gattttaacg taacagtcac aattgaaacc attaaataac aataattgtg
     4261 gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc gcgtcattca
     4321 ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt ttccccaggc
     4381 ataaagtgca cttcgttatg gttgtcggca gagatttttc ctttttatta ctgcaggaat
     4441 actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat cggtactatc
     4501 attacccagt ttgcgctggg gtcggtttat acctggagcc tgtttaatgg cgcgctttcc
     4561 gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt aagtctgggg
     4621 ctggcaattt cgtcttctgt tgcgggcaaa ttacaggaac gtttttgcgt taaacgcgtc
     4681 accatggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca ttccaacaac
     4741 ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg cgcgggttac
     4801 ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct gatctccgcg
     4861 ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga cacgcagctg
     4921 ctcgaaacgg tgggtctgga aaaaacattt gtgatttggg gagcgattgc gctggtgatg
     4981 attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa aaccagcaat
     5041 ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca gtactggatg
     5101 ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg ggtagcgaaa
     5161 gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc agtaacggtt
     5221 atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga caaaattgcc
     5281 cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc ggccctgctg
     5341 tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc atttaacttt
     5401 ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct caataacctg
     5461 gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt tggttcgatt
     5521 atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc cctgctgatt
     5581 ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt gcgtgaggcg
     5641 catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt gtgcatcaaa
     5701 cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa ctggcccttt
     5761 ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta aatttgtgct
     5821 tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat tcgcgcgtgt
     5881 agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa aagccgttaa
     5941 gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc aaatcgatta
     6001 cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg aatggcgcgg
     6061 ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa ggcgtttctg
     6121 ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt cttctatcgc
     6181 tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt tccgcaggtg
     6241 ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc gcttccgtca
     6301 tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt ctctggttaa
     6361 tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc tacaccttgc
     6421 tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggcggtcgtc gtactggcgg
     6481 gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa gtggagaggg
     6541 cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat ctgccatcaa
     6601 actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct tccgataata
     6661 attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac gttgatgaga
     6721 cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt attttcggct
     6781 atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg ttccgtggtt
     6841 actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt caggggggcg
     6901 cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt ctgaagcagt
     6961 taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac agcaacacga
     7021 tggcggacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat cgtggcaagc
     7081 cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc aacccggatg
     7141 gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc cagcgttatc
     7201 cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc tgtaccaaag
     7261 cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt atctccagcg
     7321 tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac cacggtgagt
     7381 caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg ccggagcagt
     7441 tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg gccaatgcgc
     7501 aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt cacgtagagc
     7561 tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga attaacgaaa
     7621 acaacaactg gtgtcacatc ccgcagacaa aagaggcagc ggctaactaa gaggcctgct
     7681 gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg cgtatctgcg
     7741 cagtaagatg cgccccgcat tcggtgattg gcgcagcttg gtagcgcact tcgttcggga
     7801 cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc ctgctcagcg
     7861 agcaggcttt tttgcatttg aagcccatga ggatgagaac ctccgggggc
//
LOCUS       DAEEKQ010000177         7851 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.179, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000177 DAEEKQ010000000
VERSION     DAEEKQ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7851
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.179"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..903)
                     /gene="nac"
                     /locus_tag="KLK57_003573"
     CDS             complement(<1..903)
                     /gene="nac"
                     /locus_tag="KLK57_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HBE0893847.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAKMNLFLPSDYSAIRLRVDEAFSLRRFTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQW"
     gene            1230..1305
                     /locus_tag="KLK57_003574"
     tRNA            1230..1305
                     /locus_tag="KLK57_003574"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1263..1265,aa:Asn,seq:gtt)
     gene            complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="KLK57_003575"
                     /pseudo
     CDS             complement(1361..2297)
                     /gene="ldtA"
                     /locus_tag="KLK57_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
     gene            complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="KLK57_003576"
     CDS             complement(2359..3438)
                     /gene="cobT"
                     /locus_tag="KLK57_003576"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HBE0893848.1"
                     /translation="MQTLADLLNTIPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGVPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVYVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRHLAEKLLLDVICYT
                     RELAKNGVTLFGVGDLGMANTTPAAAIVSTITGRAPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="KLK57_003577"
     CDS             complement(3450..4193)
                     /gene="cobS"
                     /locus_tag="KLK57_003577"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HBE0893849.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="KLK57_003578"
     CDS             complement(4190..4735)
                     /gene="cobU"
                     /locus_tag="KLK57_003578"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HBE0893850.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWHTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            complement(4869..5306)
                     /locus_tag="KLK57_003579"
     CDS             complement(4869..5306)
                     /locus_tag="KLK57_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001524956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HBE0893851.1"
                     /translation="MKTIKPESIALFCLLSGDVDLAKHLAEILPITCFVGEDWQQDFQ
                     PLDGGPVQSLSDAYSDYSVLLLIAAESEISVPEWLAHSEGTTVILIAEHNARLLTGPA
                     GGAVALIHYLNEICHSRETAVNDAFCRTLTRGSLAIPQCHLLS"
     regulatory      complement(5524..5699)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            complement(5788..6464)
                     /locus_tag="KLK57_003580"
                     /pseudo
     CDS             complement(5788..6464)
                     /locus_tag="KLK57_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460981.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PocR ligand-binding domain-containing protein"
     gene            complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="KLK57_003581"
     CDS             complement(6667..7476)
                     /gene="pduF"
                     /locus_tag="KLK57_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propanediol diffusion facilitator PduF"
                     /protein_id="HBE0893852.1"
                     /translation="MNDSLKAQCVAEFLGTGLFLFFGIGCLCALKVAGASLGLWEICI
                     IWGLGISLAVYLTAGISGAHLNPAVTIALWLFASFPARKVLPFCLAQLAGAFGGAVLA
                     YSLYSSLFTDFESAHNMVRGSAESLQLASIFSTYPAAAINVWQAALVEVVITSMLMGL
                     IMALTDDGNGVPKGPLAPLLIGILVAVIGASTGPLTGFAMNPARDFGPKIFTWLAGWG
                     EIAMTGGRNIPYFIVPIIAPIIGACVGAAIYRYLIAKNLPVNSVTPKGISE"
BASE COUNT         2210 a         2082 c         1665 g         1894 t
ORIGIN      
        1 ccactgcctt ttttccatca ctggggaact aatcactgac attaacaact ctttcactgc
       61 ctgtgcctgt ggcgataagt tcgctctggc gggtaaattt aatgacaaag agagactcat
      121 ggaaggagtg gtaatgcgtg acatccaccc atttactgcg ccacataacg aacgcgcggc
      181 cgattcgggt aatactgcaa cgcccatgcc gctggcaatc gctgcggtaa gcgtggcaat
      241 agactcaatt tcaccaatga cttttgccgt gaatcgccgt aaggaaaaag cctcatcaac
      301 acgaagtcta atagcactgt aatcactggg gagaaagagg ttcattttcg caatagcatt
      361 cacatcaacg ctttgccccg ggcaatcttg agttcctacc agaaaaagat cttctttcag
      421 caaagcctga ctggatacac cagccacagg ggaatgctca taaatcaccg ccatatcgag
      481 ttggtgattt atcaattttt cgttaagcac tgcaccacta ttttcatgaa gatagataac
      541 gatctccgga aattcagcgc gaaccgcctg taataagggc atggtgatgg atgacgcagc
      601 ggttcctggt gcaaagccaa tcgagacttg ccccgataat gcctgaccaa cgttatgcac
      661 cgccagttgg gcctgttcac actgacgtaa aatggcccgc gcatgggtat agagaatttt
      721 tccggcgtct gttggcgtaa cgccccgctt tgtacggatc aaaagttgtt gatttaactc
      781 accttccagt gtggcaacct gctggctgag cgctggttgt gcgatatgca atacttcagc
      841 agcctgggtc aggctaccaa tatctacaat ttttacgaag tatttcaggc gtctgaagtt
      901 catgttgcct ccggttttta agaatcggcc caagtgccgc cattacttac aaccagattg
      961 caagatgctt gccagtttta ttttggcgtt gatgtacaag ctaaccaact gtcaaataag
     1021 agattatgat agattcgtca tttgctcctt taatcagctg tcgcgttccc ctgccctata
     1081 aaaggagggt atgcaccacg atggttcatt acccaataag attgaaagct caccactttg
     1141 ttgaaattga cagcaaacaa acaaaaaaat gcatttcacc ctttgacatc accatgcact
     1201 gccattaata tgcgccccgt tcacacgatt cctctgtagt tcagtcggta gaacggcgga
     1261 ctgttaatcc gtatgtcact ggttcgagtc cagtcagagg agccaaattc aaaaaagcct
     1321 gctttctagc aggctttttg ctttctaatt accaacgctc ttaaaacatc tgtcttgaac
     1381 cagaactaat ttgcacaggc attcccgatc gacgttgcaa cgcagcattt gcgcgattta
     1441 catcaacttc ttgcccgctg ataaacgccc gcaaagatgg ggttaccggc aatggcactt
     1501 ttcggtcaga ctcatattct gcacgattgc gcgacaacgg ctcatgaact tccagccagt
     1561 tcgagccatc tggttcagtg gtgtatttta ctggctggtc gataatttgc acacgcgtcc
     1621 caacaggaac attatcaaac agatatttga tatcgtcatt gcgcagacga atacagccct
     1681 gacttacccg gagcccaata ccaaaattgg cattggtacc atggatggca tacaacctgc
     1741 caatataaat cgcgtacagc cccatgggat tatcggggcc cgcaggaaca aatgcgggca
     1801 aactctcccc tcgtttcgca tattcgcgcc gagtgttcgg cgttggcgtc caggttggag
     1861 cttcttgttt acgttcaacg gtagtcaccc agttacgcgg ggtttctcgc ccagcctggc
     1921 cgataccaat aggaaagact tccacagtat tactgtctgg tgggtagtaa taaagacgca
     1981 tctcagcgac gttaaccaca atccctttac gaacagtggc gggcaaaatc agttgctgcg
     2041 gaatggtgag ttgcgagcca gacttcggca aaaaaaacat cagcgcccgg gttcgcttcc
     2101 agcatgttac ttaacccttg cccgtattgt gcggcaaaag tctccagcgg ctgggtattg
     2161 tgatcaggaa cagttacagt aagcgactgc cccactaaac ggctaccctc tggaggtaat
     2221 ggataagtta ccgccaggct attatggctg gcaaaaagca gagcaaatga gcaaaaaata
     2281 tttacacgac gcatcatgtc cctttcctaa gtcgcgaaag ctatccgtta agtatagctt
     2341 ttatcagact tttcgttttt aactgttcaa atcagaagtc gtattccccg gtagaacaat
     2401 attactggca gcaagttcgc ccatgttgtt atatatcgca caggcagctt cgatgatggg
     2461 catcgccaga gctgcgccac taccctcacc taaacgcatc tccatattga gataaggctc
     2521 cagccccaaa tgcgacagcg caatacgcgc ccctttttct gccgacaggt gagaaggaat
     2581 gagatacggt ttgattgcag gagacatctg gcaggctgcg agcgcggcag cataagaaag
     2641 aaatccatcc agcaacacgg gtaaaccgca ggaagcagcg cctaacatca cgccagccat
     2701 tccgaccaaa tcaaatccac ccacttttgc caggacatca acgccatcct gcggatttgg
     2761 ttgattcaac gtaatcgccc ggcgcacaac atcaatttta ttagccagtt tatctgtcgg
     2821 caggtttgcg ccaatcccaa ccacttcttc aggagcccgg ccagtgattg tgctgactat
     2881 tgccgctgcc ggtgtcgtgt ttgccatccc caggtcacct acaccaaaca gcgtaacacc
     2941 gttttttgcc agctcccgcg tataacatat gacgtccaaa agcaactttt ctgccagatg
     3001 gcgactcatt gccggagctg aagcaatatt gccactacct cgagcaacac gcatgttgat
     3061 aagcccgggg ataggctcag cagtatcaat accaacatca attacgtaga cgttagcgcc
     3121 cgcttgtgct gccagcacac acacgccggt tgttccgcgg gtcatatttt cagcctgaat
     3181 ggctgtcact tcttttgggg aaatagcgac cccttcctcc cagacgccgt gatcggcaca
     3241 catgaccagt accgcttttt tgcccacatg cggaacgcca ttcaaccccg gcattcctgc
     3301 cagttgtatg gcaagcgcct ccagccttcc caggctacca acaggtttga gtaacccgtc
     3361 gatatgccgt tgtgcacgcg acatagcggc aggatcaata gcagggatcg tattcagtaa
     3421 atcggcaagt gtttgcatgt cacgtctctt tatagcagtg ccagcaggaa gaccagttca
     3481 ccaagctcta tagcagctcc cagcgtatcg ccggtttgcc cgcccagcgt acgtttgaga
     3541 agctgaccga ggatgaaaat cgccaccatc gtcaccacca tagcagccac accatgcata
     3601 cccggcaata atacggcagc aaaaatagcg gctaagccga gtgtgacgca ggtttgtcgc
     3661 ccatcaattt tgccaatgaa cacattgcca agtccttctt cccgggcata acgatgacga
     3721 tacatcaata atgcggcagt cccgcggctg accgcacatg ctgccgccag cgatgcaagg
     3781 atcggttcgc cacgcagagc caactcgctt aataccagaa tctttgccag tacaacaaaa
     3841 atcaatgcca gaccgccgtg ggtgcctaaa cggctatcac gcatgatttc caacatgcga
     3901 tcgcggctac gtgcagaaaa tacgccatca caggtatcgg cgaggccgtc caggtggaat
     3961 cccccggtca tcagcgccag cacaagtacg ctaaacagtg ccgccagtgg tacgccacac
     4021 catgcctgca gcaccatgaa gaccagcccg ctaatcgcgc caagcaataa tccaattaaa
     4081 ggaaaagtaa taataccgcg agaataatgc tcgaaatcca gtccctggga ccagcgacgc
     4141 ggtacaggca gacgcgtaat aaaagagagc atcgcccaaa ataatttact catttgattt
     4201 ttactccaat acccgaaacc accaaccata cttcatttgc cgcagcggcc agctgctgat
     4261 ttacccgccc ggcaatatca cgaaaatgtc gtgccagacg actctccggc acaatcccca
     4321 ttcccacttc gttagtcact aatacaacct ttgcgggaca acgttggcag gcagcaatca
     4381 acgactgaat ctcagcatta atcgcctgtt ccatcgcctg ataatcccat tcatcagggt
     4441 ctttatcgcc gccataatca aacaacagat tagtcaccat ggtggtaacg cattccagca
     4501 atacagcctc atgcgggtta atgtctgcat gaattaattc atcaagatgt tgccagcgct
     4561 ccactgtgtg ccagtgcgcc gggcggcctt gccgatgatg ttctatccgt gcagccatct
     4621 catcatcaag gatttgtgag gtggcgatat acagaacctg cgaagagtcc ccaataagcg
     4681 cctctgcatg gcgactcttc ccgctccgtg cgccccccgt caccaaaatc atcataccga
     4741 ctcctgattc aattgtatga tggtattcat tgaacagaac aatggatccc ccaccgttgc
     4801 tgaccgcaaa acatttacgc gattaatact gcgcgtaata taattaaaaa tccagcattc
     4861 tcaatccatt acgaaagaag atggcattgc ggaatagcca gacttcctcg tgttaacgtc
     4921 cgacaaaatg cgtcattgac agcggtttct ctggaatgac agatttcatt cagataatgg
     4981 attaatgcta cagccccacc agccggacca gtcagcaaac gggcattgtg ttctgcaatc
     5041 aaaattaccg tcgttccctc agaatgagcg agccattctg gtacggatat ctcgctttca
     5101 gctgctatca atagcagcac tgaataatcg ctatacgcat ctgataacga ttgcacaggc
     5161 ccaccatcaa gaggctggaa atcttgctgc cagtcctcac caacaaaaca ggttattggc
     5221 aatatttcag ccagatgttt cgccagatcc acatctccag ataagaggca aaaaagagct
     5281 atcgattcgg gttttatcgt tttcatgcct ctcgccctcc acttaatttc ctgtaatggc
     5341 ggtctggttg ataagccatg aaacaatatt gccacacctt gaatgcagcc ctggtgcctg
     5401 tcggcactgc gatgaaagaa ctaaatgatg ccgccatacc ctgatccttt cagggtggta
     5461 ggggtataaa gacgcgccag tactctaccg gacgcttccg ggcaacccac ttcccaccga
     5521 agttgttggt attgactgac agactggtct tctggcttag cgtcatcctc acccgacctt
     5581 cccaatctcg cgatcagtgg cattttcggg tttgtcagca ttacagcagc gggggctgcg
     5641 agggattttc accctcttcc caggcacaaa tgtgccacac ctgtcagcgt atgttatgag
     5701 ctgatttcac ttttcccata acacgtaaat aacgttaatt attcattcca gcgtttattt
     5761 aaaccttccc tttttttagc atttattcta taccgtttcc atcgccgcat tttcactcac
     5821 ttgctgtcga tacacctgcg gcgtaacctg ataagtctga cggaaaactt tgcaaaaata
     5881 actgtattgt cgtggggatt aggcgcacga gtctggcgca ccgaatctga ccgagatttg
     5941 ttctctttaa taaccacaaa attgagttgt tttttgaggc aattttccac aatcaacttc
     6001 aacaaatcag cagaggccat aactcttgag taatccatct caggaacttt gcggaattca
     6061 ttaagtaatt cgggatctgc ctgccagcaa tcatcgacat tcagaatatc gaccaaatcg
     6121 acatcatcat tacgcagacg aacttgcccg cacaatacaa aaccgactaa atgtccggcg
     6181 attaccagtg gaatagagaa atcggttaat ccggcatgac agcgataaat acaaggctga
     6241 tcggatttag aggcttccag gcccccacaa cgatcgctca tgcgacatcg agtgcttagc
     6301 tgagggtgtt gccgcatcat ctgacaaaat ggagtgaagt taaacagctc ggaaatttca
     6361 tcgccgtgaa tattgaccac gacaaccgca aggctggtcg cctgcgcaaa atcctgcgcg
     6421 attttattaa tgagttctga attaagtgtc gccgcagaaa tcatgataaa acctcccagt
     6481 taatttattg ttatagcaaa acaattgttt tacatttaat cttcacatca tccctgatga
     6541 tttactgacg agaagcacaa aggacaataa caaaagcctc cgtgaagcgg gagacttttt
     6601 aataagttcg gaaagcagat cacaaacagt aaaaaaataa atgtttataa ctgccagagt
     6661 atttcgttat tcgctaattc ctttaggtgt aacactattg acaggtaaat tctttgcaat
     6721 gagatagcga taaatcgccg caccaacaca tgccccaatg atcggtgcaa taatgggtac
     6781 aatgaaataa ggaatattgc gtccaccagt catcgcaatt tctccccacc ccgcaagcca
     6841 ggtgaaaatc ttaggtccaa aatcacgcgc tggattcatg gcaaaaccgg ttaatggtcc
     6901 ggtagaagca ccaataacag caaccagaat accaataagt aaaggtgcaa gcggtccttt
     6961 gggtacgcca ttaccgtcat cggtcaacgc cataatcaac cccattaaca tggacgttat
     7021 gaccacttcg accaacgcag cttgccatac attaatcgcc gctgccggat aagtactgaa
     7081 gatactggcg agttgtaaac tttctgcgct accacgcacc atattgtgag cagattcaaa
     7141 atcggtaaac agactactgt atagagaata tgccagaact gcaccaccaa aggcacccgc
     7201 taattgcgcc agacaaaatg gcagcacttt acgggctggg aaagaggcaa acagccacag
     7261 agcaatggta actgccgggt tgagatgagc gccggaaata cctgccgtaa ggtaaacagc
     7321 aagcgaaatc cctaaccccc aaatgataca aatttcccac agtcccagac ttgccccggc
     7381 aactttcagg gcgcacaaac aaccaatgcc aaaaaagagg aacagtccgg tgcccaaaaa
     7441 ctcggcaacg cattgcgcct tgagtgaatc attcatagtg acaccttttt tacggagttt
     7501 ttctttatca gccaactatg cgctgactgt tgtatatacc gagcacaact ataaaaaatt
     7561 gctctgcaga aaatattgcc aacatgcatg acagactcgt gtaaaaatcg tgctaacata
     7621 acaaattatt gtcattaccg aatcatgcaa cacagcgtta gtcagtgttg aaagtaataa
     7681 ttcccaccaa acttttacaa taaaaaattg ccatcatgac ataataaaat tgaaacaaat
     7741 atcacagttc cttcttcgaa ctaaactcaa ccaaatatta ctcgcgatta aactcactag
     7801 ccaacgcaat taattcacat tgaaatatcc acaaaacaac caataaatta a
//
LOCUS       DAEEKQ010000178         7835 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.180, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000178 DAEEKQ010000000
VERSION     DAEEKQ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.180"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            209..937
                     /gene="gadW"
                     /locus_tag="KLK57_003582"
     CDS             209..937
                     /gene="gadW"
                     /locus_tag="KLK57_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HBE0893853.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(1300..4413)
                     /locus_tag="KLK57_003583"
     CDS             complement(1300..4413)
                     /locus_tag="KLK57_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HBE0893854.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(4438..5595)
                     /gene="mdtE"
                     /locus_tag="KLK57_003584"
     CDS             complement(4438..5595)
                     /gene="mdtE"
                     /locus_tag="KLK57_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HBE0893855.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            5655..5933
                     /locus_tag="KLK57_003585"
     CDS             5655..5933
                     /locus_tag="KLK57_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893856.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYLGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(5934..6461)
                     /gene="gadE"
                     /locus_tag="KLK57_003586"
     CDS             complement(5934..6461)
                     /gene="gadE"
                     /locus_tag="KLK57_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HBE0893857.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(7260..7832)
                     /gene="hdeD"
                     /locus_tag="KLK57_003587"
     CDS             complement(7260..7832)
                     /gene="hdeD"
                     /locus_tag="KLK57_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HBE0893858.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2073 a         1866 c         1843 g         2053 t
ORIGIN      
        1 atttttttat aaacataagc tatacgctgt gcgaaaatat attcgtgctg catttactta
       61 ttatcaatta actgttatgc aaaactactt tgtggataaa ttttggtcct accaaatctg
      121 gcagtttttt cgctaagaaa cagtctggca acatttcatt agtatactga aattgaaata
      181 atcgcagtat gaaatataag ggataatcat gactcatgtc tgctcggtga tcctcattcg
      241 tcgttcattc gatatttatc atgaacagca taaaatatcg ctgcataacg agagtatcgt
      301 gctgctggag aaaaatttgg cagacgattt tgcgttttgt tcaccggata cgcgacgact
      361 ggatatcgat gagctgacag tttgccatta cttacaaaat attcgtcagc taccacgcaa
      421 tttagggtta catagcaaag accgtctatt aattaaccag tcacccccca tgccgctggt
      481 gacggcgatt tttgatagct tcaatgaatc cggggtaaat tcaccgatac tgagcaatat
      541 gctctacctt tcctgtttat cgatgttttc tcataagaaa gaactgatcc ccttactttt
      601 caatagcatc agcactgttt caggaaaagt tgaacgcctt attagctttg atatcgccaa
      661 acgttggtat ctgcgcgata tcgcggaaag aatgtatacc agcgagagtc tcataaaaaa
      721 aaagttgcag gatgaaaata cctgtttcag taaaatatta ctcgcttcca ggatgtcgat
      781 ggccagacga ttactcgagt tacgtcaaat tcctctgcat actattgcag aaaaatgtgg
      841 ctatagcagt acgtcgtact ttataaacac atttcgacaa tattatggtg taacgccaca
      901 tcagtttgcg caacattcgc caggtacctt ttcctgacat attttgcatt tgaatattgg
      961 tcaggatctc acacctgctt catgtgaaac tcttccctga tgatttctgc cgggctaccg
     1021 gctagttctc tttcgcagtt tgccaggcgg tataggtatt ttcaggagga aaagactcgg
     1081 catgtttggg attattaagc tgacaattca tacgattacg ggaatgtggg tcagtattgt
     1141 gctgttcaaa ttgatgacga atggctggag tggtttctac tttcagtgct gtgtactaag
     1201 cctggtattc ctgacggtta gctggttgtt gagtggtgaa tggttagcag gaaagagtaa
     1261 ggctgaacct tcacgttcaa ccttactctc atttacacgt tacgcttttt taaagcgggc
     1321 aaagagatgt tccactacaa cgaaaaagac cggaacgaag taaattgcca gcactgttgc
     1381 ggcaaacatc ccgcccatca cgccggtacc taccgcgttt tgcgcgccag aaccggcacc
     1441 atgactgata accagcggca gcacgccgag aataaaggcg agagaggtca tcaggattgg
     1501 gcgtaaacgc atccgcgccg cttcgatgat tgcctctatc ggcgttttcc cttctttctg
     1561 catcatctca acggcaaatt cgacaatcag gatggcgttc ttggcggaaa gcccgatggt
     1621 ggtcagcaaa ccaacctgga agtagacgtc attacttaag ccgcgcagat cggtggccag
     1681 taatgcgcca acgacgccta acggaacaac caacatcacc gagaacggaa ttgaccagct
     1741 ctcatagagt gcggcgaggg cgaggaacac cacgaccagt gaaatggcat acagtgcagg
     1801 agcctggttt gaggataacg cttcctgata cgacagtccg gtccatgagt agccgacgcc
     1861 tgccggaagt ttagcgacca ggtctgccat aaatttcata gcgtcaccgg tacttttccc
     1921 ggccgccgct tcacctaaaa tctccattga cgggatgccg ttgtagcgtt ccaatcgcgg
     1981 tgaaccatag gtccattcgg tagacgagta ggcagaaagc ggtgccatcg tgccagaggc
     2041 gttgcgtaca taccattggt tgatgttatc cggcaacata cggaacggcg tgcctgcctg
     2101 gacatacact tttttcaccc gcccctggtt gaggaagtcg ttcacgtagc tgctgccgaa
     2161 ggcggtggaa attgtctggt tgatatcaga cagcgccaca cccattgcct cagctttcgc
     2221 ggcgttgacg ttcactttga acatcggcgt atcttccagg ccgttcgggc gtaccccggt
     2281 gacctggtca ggcgattgcg ctgccagtga taacagctcg tttcgtgcct gggttagttt
     2341 ttcgtgcccc aggttaccgt tgtccagcag ttccatatca aaacctgacg cggtacccag
     2401 ttcagccacc gcgggtaagt tgaacgggaa gacgacggct ttattgatac tgcttaacgc
     2461 aatcattgcc cgctgaatga tcgcggtaac cgagttttcc tcaccgacac gttcagacca
     2521 cggcttgaga ctgataaacg ccaggccgtt gttttgcccc tggccgctga agccaaagcc
     2581 gccaacggta aacaccgact gaacattatc tttctctttt gtaagataat aatccgtcac
     2641 ctgttgcagc actttcgtgg tgttaaccat ggtggcaccg gaaggtaact gcgcggtggt
     2701 cataaatacc ccctgatcct cttctggtaa gaaagaggtc ggtgtacgca ggaacagcac
     2761 cgccatcccg gcgcaaatca gcaggtagac caccatgtag cgaccggtac aacgcaacag
     2821 cgagcgggtg ctatcggtat agtgctgagt tgatttttca aacagcgtgt tgaagcgtgc
     2881 gaacagggcg ttaggtttgt gaccgccttc cggcgcggct ttcagaatgg tggcgcacag
     2941 ggcaggggtc aggctcattg ccacaaatac tgaaagcagc atggaggaga tcagcgtgat
     3001 ggagaactgg cggtagatct ccccggttgc gccgctcata aaggccatcg gcataaacac
     3061 tgcggaaaga acaacagcaa taccgaccag cgcacgttgg atctgcccca tcgatttatg
     3121 cgtcgcttcc ttcggtggta gcttatcttc cgcaatgaca cgctcgacgt tctccaccac
     3181 cacgatggcg tcatccacca gtaaccctat cgccagcacc atcccgaaca tagttaacgt
     3241 gttgatggtg aaaccgaccg ccgacaagat cgcaaacgtc ccgagaataa ccaccggtac
     3301 ggcaatcgtc gggatgattg tggcacggaa attctgcaaa aacagataca tgaccaggaa
     3361 gacgaggatg atagcctcaa ccagtgtttt aaaaacttcc tgaatagaaa tttcgataaa
     3421 cggcgtggtg tcgtaaggat aaaccgtctt cagacttgcg gggaaatagg ctgataagcg
     3481 gttcagctcc tctttgactg cccgcgaggt atccagggcg tttgctccgg tagccagttt
     3541 gatggcgatc ccggcagcag gtttgccgtt atagcgcgcc acggtggaat aatcttccgc
     3601 tccaagttcg acgcgggcga catcacgcag cagcacttgc gaaccatctt gctgaacttt
     3661 caacaggatt ttgccaaatt cttccggcgt ttgcagacgc gtctgcacaa tgatcgaggc
     3721 gtttagttgc tggtctgccg cctgtggcat gccacccagt tgaccaccgg aaatctggtt
     3781 gttttgcacc ttaatctggg aaataacatc ggaaggtacc agattgtatt tattgagttt
     3841 ttgcgggtcc agccagatac gcatggcgta ctcggaacca aagagctgta cgctaccaac
     3901 gcccgcggta cggcttagcg ggtctttgat attagacgct acatagtccg cgatatcgta
     3961 ctggttgagg ctgccgttat cagaaataaa cgccgctacc atcagaatat tactgctcga
     4021 cttatcaacg ctaatcccct gctgctgcac cgcttcaggt aatgaaggca tagcgagttg
     4081 cagtttattt tgcacctgaa cctgtgcgat atcaggagat gtcccagtct cgaaggtcag
     4141 agtgatagag gcattacccg ccgcatcact ggttgaagac atgtacatca ggccatcaag
     4201 cccattcata ttttgctcaa tcacctgagt gaccgagtct tctaccgttt gcgcatcggc
     4261 acctggatag gtagcgctga cggtaatggt cggtggcgca atctgcggat actgcgcaac
     4321 cggtaagttc atgatcgcca gaccacctgc aagcatcata ataatggcaa gtacccaggc
     4381 aaaaaccggg cgatcaataa aatagttagc catgaaagtc cccttaagcc tgcaacgtta
     4441 ttgtttcgat tcggtgctgg cgttttcctg gctggaggaa attgctcgtg ctttgatacc
     4501 cggacgaatg cgttgcaaac cggaaacgat cacccgatcg ccagcctgca agccagaggt
     4561 gacaacccac tggtcgccga tggctttgct ggcttcaatt tcgcgtagct gcacaacatc
     4621 gtctttatcc agaatgagcg ccgttgcttt accctgggcg ttgtgagtga cgccttcctg
     4681 cggcaccagt aatacattct ggcggctacc ttcatccact aatgccgtga cgtacatgcc
     4741 aggcagcaag tcaccatttg ggttggggaa aatcgcccgt aacgtcacgg agcccgtggt
     4801 ttcatccacc gtcgggtcgg agaatttcag tgtgccggtc tggctgtagc gtttaccatt
     4861 ttccagattg agctgtactg gcgtactgcc ctgaacctgt ttgatttgcc cactggcgac
     4921 ctcttctttc atgcgcaaga aatcctgcac cgactgcgtg agatcgacat aaatcgggtc
     4981 cagacgttgt acggtaacca gcgaatctgc ctgattagcg gtaacgagtg cgccgacggt
     5041 caccgacgat ttcccgctga cgcccgtaat cggcgaggtg acattcgcgt attgcagatt
     5101 gatcgtcgcc tgttcaacag ccgctttggc gacggtgaca ttggcttctg cttcattcag
     5161 ctgggtgcgc gcggtgtcgt aatcctgacg gctaacgtag ttggtcttca gcaacgatgc
     5221 ctggcggtta aaggtgatgc gggcattgct ggcggtagag agcgctttcg ccagcgagcc
     5281 tttggcggag tttagctcgg cctgtaaagg tgcaggatca atctgataca gcgaatcgcc
     5341 ctggttcact ttatcgcctt cgataaagtt gcgtttaatg ataataccgc ccacctgggg
     5401 acgtatctcg gcaacttcat aaggaacggt tctaccgggc aattcgctca acacattgac
     5461 cgaaccgggg gagagtgtga cgacaccgac ctcaggcgtc atggcggcgg cgttttccgc
     5521 cgatttgtca tcgcaggcgg tgagcatcgc gccgcagaat aacaacggta ttaacagctt
     5581 tcttcttctg ttcattttag tccctgaaaa ttcttgagaa ataacggttt tttaaattgc
     5641 gcgcgaggag agatatgagg gatttacaga ccagcggcat agtggggttg tcagccagta
     5701 aggtgggata caggcacagt gatcgacatg gtgaggtcaa cgacaaaaaa aatgttgccg
     5761 ttctgccaac agttcctgcc agcattcggg caacgaaggg ttctactggt ggatacacat
     5821 acctggggaa taaagtcgtt ttagcctctc ctttagtaat caccgggggt aatgaaatca
     5881 gcatctgcat ccctcgtcat gccagccatc aatttcagtt gcttatgtcc tgactaaaaa
     5941 taagatgtga tacccagggt gacgatgtcg ctcatacgtc ttagtttcag cttgtacata
     6001 atgttgaatt tctgaatctt taatgtttgt tgcgaaatat tgagcgtcga cgtgatattg
     6061 ctttcatttt tatttttcag aatcagttgc ataattttga actgattatg tgaaaaacat
     6121 aattgtgaat tcttatgggg caagtgttta ccataagtaa tctcaagaag atttttgata
     6181 ctttctttgc ggctaacaaa ataaataggc gctttagctt ttagtttact gatgtgataa
     6241 ggtgccaaaa caataattct ctcggcatct aatttctcca gaaatttaat cgcttcttca
     6301 tcaaggatat gattgtgata ggtatcaatg ataaccttga agggtttatt gcctactttt
     6361 ttgatctctg acagggaatt gatttttatc atttcgtgat tatctttcaa ctgccaaaag
     6421 ccctgtaaaa gaaaagaatc tttcgtcatg agaaaaatca taacttgctc cttagccgtt
     6481 atcgtttcga acatgtcatc cttttcatta tttacatcct tgtccgaatc gttgttcaat
     6541 atagtaaacg cctataagaa ctttaccctt cgtgaactaa tacgtctatt aacactaatc
     6601 aatacttcag atttatccat cacgcactcc atatattgat cctaagtaag attcctggtt
     6661 gttatcagct tgtaaatagt aaacaaaaag ttaacgtttg cggattactg cccaagaata
     6721 agttatcaat aaccagtttg tgatctctga agaatattac taaagttaaa atgtaatccg
     6781 atttaaatat cgagtctcct tgtttcgact taagctggca attggattgc cagctttctt
     6841 ttaacgcatt gaaattacct gagtaaataa cgcattcatg tcttcaggtt tttatatagc
     6901 gtctggagac acggatattt atgcaatgta atcaacgcca gttgcaaaaa aatgaacccg
     6961 gattacttat ttaaccgccc atagcatcaa cgcttacgga actctttgtt cactttccca
     7021 cagcactttt aaaacattaa acctgactac ggaaaatatc agccatgata atgtttgaaa
     7081 tggctaattt gccatggagt gaaaaaaatt agatgaaatt cagtaggttg aaataatcac
     7141 tagcaggtaa ttatttcaat gatagtgcgc aattgatcta caacactccg tagcggagag
     7201 agtattaatc ggatcatagt catatcaagt gactatgatc cgggtgacaa ccgggataat
     7261 tattgctgct taacgaacaa actggcgaag ctgaacaggc tggcggcgct aaatatcagt
     7321 tcaattccca ccagtgtgga aaccagcgtt acagacacca tcggcgttgc accaaggaat
     7381 atccaggcaa tgacgatatc cagcacacca ataacgagct gtagccagct gcctttcatt
     7441 gaacgctgac gataccaact catcaggcga ataacccctg caacacagaa caaaccggca
     7501 ataaatgccg caatggcaaa aatgcccagc tccggtgcgc ggatgaagaa atagccgatc
     7561 aataaatagg cgactgcgac gaggaaaccg gataataccg gccagaaatt atgactgcgg
     7621 ttgctgaata acccgacaat aagcgcaatg cccgagcaga ttaataatgc acccactact
     7681 gtgcttaaaa tatcgccaga gacgaacggg aaactgatac acagcaaccc gacgataaac
     7741 agtagcacgg caataaactg gattgctctg cgatgttttt taagcatctc cagatcaaac
     7801 ttcaaaattg ttgccttatc tatatataac ataga
//
LOCUS       DAEEKQ010000179         7797 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.181, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000179 DAEEKQ010000000
VERSION     DAEEKQ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.181"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            223..1185
                     /locus_tag="KLK57_003588"
     CDS             223..1185
                     /locus_tag="KLK57_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HBE0893859.1"
                     /translation="METGKIALCSAFIFLVLFAKNELRTKIKFGKLILFASLATQLLF
                     FAHAMWQHFYLNVDRVALSASHATTAGYIILFPSLLASILILKSDFRHKTTLYTINFM
                     LSLCAVIVTETRAAILVFPFFALLLIVMDSYINKRINYKLYCFIAIALLAGVFSFKDT
                     LLMRMNNLNNDLVNYSHDNTRTSVGARLAMYEVGLKTYSPIGQSLEKRAEKIHELEEK
                     EPRLSGALPYIDSHLHNDLIDTLSTRGIPGVVLTILAFSAILIYALRTAKEPYILILL
                     FSLLVVGLSDVILFSKPVPTAVFVTIILLCAYFKAQSDQCLLDK"
     gene            complement(1255..2226)
                     /gene="rfaC"
                     /locus_tag="KLK57_003589"
     CDS             complement(1255..2226)
                     /gene="rfaC"
                     /locus_tag="KLK57_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HBE0893860.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGMDWQTAREPLASLFYNRKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQMVCRAPGNELSQLTANAVKRFIEENVAMI"
     gene            complement(2230..3276)
                     /gene="rfaF"
                     /locus_tag="KLK57_003590"
     CDS             complement(2230..3276)
                     /gene="rfaF"
                     /locus_tag="KLK57_003590"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HBE0893861.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDLRVLDKEAWPLMVERYVALAYDKGVMRTAQDLPKPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            complement(3286..4218)
                     /gene="rfaD"
                     /locus_tag="KLK57_003591"
     CDS             complement(3286..4218)
                     /gene="rfaD"
                     /locus_tag="KLK57_003591"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HBE0893862.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4432..5628
                     /gene="kbl"
                     /locus_tag="KLK57_003592"
     CDS             4432..5628
                     /gene="kbl"
                     /locus_tag="KLK57_003592"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HBE0893863.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANYPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            5638..6663
                     /gene="tdh"
                     /locus_tag="KLK57_003593"
     CDS             5638..6663
                     /gene="tdh"
                     /locus_tag="KLK57_003593"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HBE0893864.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMAEGFDIGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            6902..>7797
                     /gene="waaH"
                     /locus_tag="KLK57_003594"
     CDS             6902..>7797
                     /gene="waaH"
                     /locus_tag="KLK57_003594"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HBE0893865.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWKSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDIITRQRMIAEIFTSG"
BASE COUNT         1945 a         1851 c         1897 g         2104 t
ORIGIN      
        1 ttgatgactt tgtctttatg ggatgatgtt gcaacaaagt ttcttcatgc agctggaatt
       61 atatctgcat tgtattttct aataacacca aaaaaaacaa taactaacaa tcctactttg
      121 ttaattttca tctcattatg tcttttggga atcataaata tcatctggta ttcacattat
      181 aaagtttcag gctctgttta taccaatgca tatcgtggcc caatggaaac tgggaaaatt
      241 gccttgtgta gtgcttttat tttcttggtt ctttttgcta aaaatgagtt gagaacaaaa
      301 ataaaatttg ggaaactaat tctgttcgca tccctggcaa cgcagttact tttttttgcg
      361 catgccatgt ggcaacattt ctatttaaac gttgaccgtg ttgcattatc agcttcccac
      421 gctacaacag caggctacat catccttttc ccttctttac tggcatcaat tctcatttta
      481 aaatccgact ttagacataa aacaacatta tatacaatta acttcatgct tagcttatgt
      541 gctgtcatag taactgaaac gcgtgcagcc atattagtgt ttccattctt tgcgttacta
      601 ttaatcgtaa tggatagtta tattaataag cgaattaatt ataagttata ttgttttatt
      661 gcgattgcat tattagcagg tgtattttct tttaaagata cattgcttat gagaatgaat
      721 aacttaaata acgatttagt taattattcg catgataaca ccagaacttc agtcggtgcc
      781 cgtctggcaa tgtatgaagt tggcttaaaa acatattctc caataggaca atcactggaa
      841 aaacgagcgg aaaaaataca tgaactagaa gaaaaagagc ctagattgag tggcgcttta
      901 ccctatatag attctcattt gcataacgat ctcatagata cgttatcaac gcgtggtatt
      961 cctggagttg tattaacaat tttagccttt tcagcaatac tcatatatgc cttaagaact
     1021 gctaaagaac cttatatttt aatcttgctt ttttcactac tggtagtagg cctaagtgat
     1081 gtaatactct tttctaaacc ggttccgact gctgtgtttg tcaccataat attgctttgt
     1141 gcttatttta aagcacaatc agaccaatgt ttattagata aataatgaat atgtgaaata
     1201 aaatcagcaa ggaggctgat tttatttcaa aattatctat taaggggttg taatttaaat
     1261 catagcaacg ttttcttcaa tgaatcgctt aacagcattt gctgtcaatt gagacaactc
     1321 attccccgga gccctacaaa ccatctgatt cttcccatat ccaccaatta atcccggatc
     1381 ggttggtcca taaaccgtga tattgggtct atccagtgcc gccgttaaat ggcttaaccc
     1441 cgtatccacc gacactacaa atttagcccc ggccagcacg cgggcaacgc cttccagact
     1501 catcttcggc aatacttcaa cataagcaaa tccttccgcc agtcgtttcg cccgctcttc
     1561 ctcatgcggc gcgccccacg gaagtttaat ccgtattcct gaatcagcca gtaaaccaat
     1621 caattctcgc cagtgttctt ccggccagtg tttatcatca cgggtcgtcg catgaagaaa
     1681 tacggcatat tcgccagcat ctgtaggcag gttcgtcagg aaatgctgtg caatagcata
     1741 atcgccctgg gtttgcggtt tgctatagcc cagacttttg gcaaacagtt cgcgggtgcg
     1801 ttctacggcg tgctgctgtt ttgcaatatg atgcttacga ttgtaaaaca gactggctaa
     1861 aggttcgcga gcggtttgcc agtccatgcc atgctttacg ccatgcgcca gacgcgtcac
     1921 cagcgccgcg ctttttacca gcccctgagc gtcgataact gcgtcatagt tctctgcttg
     1981 tagcgcttca cgaaacgctt tgcgttccgc ttttatgggg gccgagaacc aggctttacg
     2041 ccagcgacgt attgccacag gaataactcg ctcaacggcg gcgtgccagg aaggaatctg
     2101 tgcgaaccct tcttccacca cccagtcaaa cttaatccct gggattgcct gctgggcatc
     2161 agtgagtgcg ggcaacgtat ggagaacatc gcccatcgac gatgttttaa cgatcaaaac
     2221 ccgcatccgt caggcttcct cttgtaacaa tagcgcgttg agttcttcca gtacgcgctg
     2281 gggagtaatg tcgattaagc tctggtgata accctccgca gcgtcacctt tacgcacttt
     2341 gtgatagccg gtaatcaggc ggatcacgcg cgctttatgg gatagcggcg gtgtgaagtc
     2401 cgggctactc ggaccataca gggcaaccag cggacgattg agcgccgccg caacgtgcat
     2461 caggccagaa tcgttagtga caatggcttt acaggctgca atcaggataa ccgcttgatc
     2521 aagctgtgtt tcccccgcca ggttccgaca ccatgcctgc tgctcggtat tcaaagcggc
     2581 aagaatctca ttgcccgctt catgatcttt cgccgagcca aacagaacca cctgataacc
     2641 ttcatcaatc agctgctttg ccagttccgc atagtggtag tgtggccagc gttttgccgg
     2701 accaaactcc gcacccgggc aaaaaccaat catcggacgt tctgatgaaa gcgaaaattg
     2761 attacaggta tatgattttt caccttcgct cacctgcaat tgcggccata acaacggttt
     2821 tggtagatct tgtgcggtac gcatcacgcc tttgtcatag gccagcgcga catagcgttc
     2881 caccattagc ggccaggctt ctttatcgag cacgcgtaaa tcgttgagta aaccgtagcg
     2941 catctcgccg cgccagccgg tgcgatgagg aatacccgcg aagaaaggca ctaatgcaga
     3001 tttgaaggag ttgggtaaga cgtaggcgcg gtcgtagcgc ttttcacgca ggctatgacc
     3061 cagtttgcgg cgttcgccga tttccagcgc tccgtgaccg agaggcatag gaatagcttc
     3121 gttaacttcc ggcatccgcg ataataatgg acggcaccat gccggtgcca tcacatcgat
     3181 tatcgcctgg ggatagcgcg cctggagcgt gcgatagaga ctttgcgaca tcatcatgtc
     3241 gccaacccaa gacgggccga tcaccagtat tttcatgcag agctcttatg cgtcgcgatt
     3301 cagccaggcc atgtattccg ttacaccttc agcaacggtt ttgaacggtt tgtcgtaacc
     3361 cgccgcgcgc agatttgtca gatctgcctg agtgaacgcc tggtagcggc ctttcagctt
     3421 atccgggaac ggaatgtatt cgatctggcc tttcttgtga taagccagcg tagcatcagc
     3481 tacagcctgg aaggattccg cacgaccagt accgaggttg aagatgccgg aaacgccatt
     3541 ttccaggaac cacagattca catcagccac gtcgcccaca tagacgaagt cgcgtttgaa
     3601 gttctcgcta ccttcaaaca gcttcggtga ttcaccgttg ttaagctggg tgttgagatg
     3661 gaaagcgacg ctcgccatgc tgcctttatg gccttcacgc ggtccataaa cgttgaaata
     3721 gcggaagcca acaatctgcg agttagcttc aggcaggatt tggcgaacat attcatcaaa
     3781 caggaatttt gagtaaccgt agacgttcaa cggtttttcg tactcgcggg attcaataaa
     3841 gtcggaggtg cgtccgccgt aggtggctgc ggaagaggca tacaggaacg ggatttcgcg
     3901 ctccagacag tagtgcagca gctctttgga gtattgatag ttgttatcca tcatatactt
     3961 gccatcccac tcggtggtgg aagagcacgc gccttcgtgg aaaatcgctt cgacatcgcc
     4021 gaactcttcg ccagccataa tctggatcag gaagtcttcc ttatccatat agtccgcgat
     4081 atccagatcc accaggttca caaacttggt gccgtctttc aggttgtcca ccaccagaat
     4141 atcggtgatg cctttatcat tcagggcctt aacgatgttg ctgccgataa agcccgcgcc
     4201 gccggtaacg atgatcataa ctgtaacctt cgaattatgg agtcagagac aatctcagac
     4261 acgaatactt ctatcatatc actacacggc gttggcttca gccattcgcc aaaagtgaga
     4321 ttgatggacg ttatttatcc tgaattttgc agaagtgtta acgcgtcatc tcgtcgcgac
     4381 ctataagttt gggtaatatg tgctggaatt tgccctgtct ggagaatcgc aatgcgtgga
     4441 gaattttatc agcagttaac caacgatctg gaaaccgcac gggcggaagg gttgtttaaa
     4501 gaagagcgca ttattacgtc tgcgcagcaa gcagatatca ctgtggctga tggaagccac
     4561 gtcattaact tttgtgccaa taactatctc gggctggcga attatccgga tctgattgcg
     4621 gcggcaaagg cgggaatgga ttctcacggt ttcggcatgg cttcggtgcg ttttatttgc
     4681 ggcactcagg acagccataa agagcttgaa caaaaactgg cggcattcct ggggatggaa
     4741 gatgcgattc tctactcttc ctgctttgat gctaacggtg gcctgtttga aacgctgctg
     4801 ggcgcggaag acgccattat ctccgacgca ctgaaccacg cgtctattat tgatggtgtg
     4861 cgtctgtgta aagctaaacg ctatcgctat gccaacaacg atatgcagga gctggaagca
     4921 cgtctgaaag aagcgcgtga agccggtgcg cgtcatgtgc tgattgccac cgatggtgtg
     4981 ttctcgatgg acggcgtgat tgccaacctg aagggcgttt gcgatctcgc agataaatat
     5041 gatgccctgg tgatggtaga cgactcccac gcggtcggtt ttgtcggtga aaatggtcgt
     5101 ggttcccatg aatactgcga tgtgatgggc cgggtcgata ttatcaccgg cacgctcggt
     5161 aaagcgctgg gcggggcttc tggtggttat accgcggcgc gcaaagaagt ggttgagtgg
     5221 ctgcgccagc gttctcgtcc gtacctgttt tccaactcgc tggcaccggc cattgttgcc
     5281 gcatccatca aagtactgga gatggtcgaa gcgggtagcg agctgcgtga ccgtctgtgg
     5341 gcgaacgcgc gtcagttccg tgagcaaatg tcggcggcgg gctttaccct ggcgggagcc
     5401 gatcacgcca ttattccggt catgcttggt gatgcggtag tggcgcagaa atttgcccgt
     5461 gagctgcaaa aagagggcat ttacgtcacc ggtttcttct atccggtcgt tccgaaaggt
     5521 caggcgcgta ttcgtaccca gatgtctgcg gcgcataccc ctgagcaaat tacgcgtgca
     5581 gtagaagcat tcacgcgtat tggtaaacaa ctgggcgtta ttgcctgagg atgtgagatg
     5641 aaagcgttat ccaaactgaa agcggaagag ggcatctgga tgaccgacgt tcctgtaccg
     5701 gaactcgggc ataacgatct gctgattaaa atccgtaaaa cagccatctg cgggactgac
     5761 gttcacatct ataactggga tgagtggtcg caaaaaacca tcccggtgcc gatggtcgtg
     5821 ggccatgaat atgtcggtga agtggtaggt attggtcagg aagtgaaggg cttcaagatt
     5881 ggcgatcgcg tttctggcga aggccatatc acctgcggtc actgtcgcaa ctgtcgtggc
     5941 ggtcgtaccc atctgtgccg caacacgatc ggcgtcggcg ttaaccgccc gggctgcttc
     6001 gccgaatatc tggtgattcc ggcgttcaac gccttcaaaa ttcccgacaa tatctccgac
     6061 gacctggctt ccatttttga tcccttcggt aacgccgtgc ataccgcgct gtcgttcgat
     6121 ctggttggcg aggatgtgct ggtctctggt gcaggcccga ttggtattat ggcagcggcg
     6181 gtggcgaaac acgttggtgc acgcaatgtg gtgatcactg atgttaacga atatcgtctc
     6241 gaactggcgc gcaaaatggg tatcacccgt gcggtcaacg tcgctaaaga aaaccttaat
     6301 gatgtgatgg cagaactggg catggccgaa ggctttgata tcggtctgga aatgtccggt
     6361 gcgccgccag cgtttcgtac catgctcgac accatgaacc acggcggtcg cattgcgatg
     6421 ctgggtattc caccgtccga tatgtctatc gactggacca aagtaatctt taaaggcttg
     6481 ttcattaaag gtatttacgg tcgtgagatg tttgaaacct ggtacaagat ggcggcgctg
     6541 attcagtcag gtctggatct ctcgccgatc attacccatc gtttctctat cgatgatttc
     6601 cagaagggct ttgacgctat gcgttcgggc cagtccggga aagtaattct gagctgggat
     6661 taacacgaac aagggctggt attccagccc ttttgcctga ggataatccg ttaaatatgt
     6721 aaaatcctgt cagtgtaata aagatatcgt aattgcgctg atctcttata tagctgctct
     6781 cattatctct ctaccctgaa gtgactctct cacctgtaaa aataatatct cacaggctta
     6841 atagtttctt aatacaaagc ctgtaaaacg tcaggataac ttcagaggtc gtcggtaatt
     6901 tatgatgaac agcaccaata aacttagtgt tattattccg ttatataatg cgggcgatga
     6961 tttccgcact tgtatggaat ctttaattac gcaaacctgg actgctctgg aaatcattat
     7021 tattaacgat ggttcaacgg ataattctgt tgaaatagca aagcattacg cagaaaacta
     7081 tccgcacgtt cgtttgttgc atcaggcgaa tgctggcgca tcggtggcgc gtaatcgtgg
     7141 gattgaagtg gcaacgggca aatatgtcgc ttttgtcgat gctgacgatg aagtctatcc
     7201 caccatgtac gaaacgctga tgaccatggc gttagaggac gacctcgacg tggcgcagtg
     7261 caacgctgac tggtgttttc gtgaaacggg agaaacctgg aaatccatcc ccagcgatcg
     7321 ccttcgctca accggcgtat taaccggccc ggactggctg cggatggggc tttcttcgcg
     7381 ccgttggact cacgttgtct ggatgggggt ttatcgccgc gatgttattg ttaaaaataa
     7441 cattaaattt attgccggat tacatcatca ggatattgtc tggacaacag aatttatgtt
     7501 taatgcgctg cgtgcgcgat ataccgagca atcgttatat aaatattatc tgcataatac
     7561 gtcagtgagt cggttgcata gacaagggaa caaaaacctt aattatcaac gtcactatat
     7621 taagattact cgtctgctag agaaattaaa tcgaaattat gccgacaaaa ttacgattta
     7681 tccggaattt catcagcaaa taacttacga agcattgcgt gtttgccatg cggtgcgcaa
     7741 agagccggat attattaccc gccaacggat gattgccgag atatttactt ccggtat
//
LOCUS       DAEEKQ010000180         7794 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.182, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000180 DAEEKQ010000000
VERSION     DAEEKQ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7794
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.182"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            258..761
                     /gene="dps"
                     /locus_tag="KLK57_003595"
                     /gene_synonym="pexB"
     CDS             258..761
                     /gene="dps"
                     /locus_tag="KLK57_003595"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HBE0893866.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            1165..1911
                     /gene="glnH"
                     /locus_tag="KLK57_003596"
     CDS             1165..1911
                     /gene="glnH"
                     /locus_tag="KLK57_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HBE0893867.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            2050..2709
                     /gene="glnP"
                     /locus_tag="KLK57_003597"
     CDS             2050..2709
                     /gene="glnP"
                     /locus_tag="KLK57_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HBE0893868.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            2706..3428
                     /gene="glnQ"
                     /locus_tag="KLK57_003598"
     CDS             2706..3428
                     /gene="glnQ"
                     /locus_tag="KLK57_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HBE0893869.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            3545..5770
                     /gene="ybiO"
                     /locus_tag="KLK57_003599"
     CDS             3545..5770
                     /gene="ybiO"
                     /locus_tag="KLK57_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HBE0893870.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALSHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLIIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPTPGATPAEPLPPGEPT
                     L"
     gene            complement(5767..6693)
                     /gene="rlmF"
                     /locus_tag="KLK57_003600"
     CDS             complement(5767..6693)
                     /gene="rlmF"
                     /locus_tag="KLK57_003600"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HBE0893871.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            6969..7229
                     /locus_tag="KLK57_003601"
     CDS             6969..7229
                     /locus_tag="KLK57_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0893872.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            7494..>7794
                     /locus_tag="KLK57_003602"
     CDS             7494..>7794
                     /locus_tag="KLK57_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBE0893873.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNAPG"
BASE COUNT         1893 a         1988 c         1948 g         1965 t
ORIGIN      
        1 ctcgctactt ttcctctaca ccgtctttat atatcgaatt atgcaaaagc atatttattc
       61 cgaaaattcc tggcgagcag ataaataaga attgttctta tcaatatatc taactcattg
      121 aatctttatt agttttgttt ttcacgcttg ttaccactat tagtgtgata ggaacagcca
      181 gaatagcgga acacatagcc ggtgctatac ttagtctcgt taattactgg gacataacat
      241 caagaggata tgaaattatg agtaccgcta aattagttaa atcaaaagcg accaatctgc
      301 tttatacccg caacgatgtc tccgacagcg agaaaaaagc aacagtagag ttgctgaatc
      361 gccaggttat ccagtttatt gatctttctt tgattaccaa acaagcgcac tggaacatgc
      421 gcggcgctaa cttcattgcc gtacatgaaa tgctggatgg cttccgcacc gcactgatcg
      481 atcatctgga taccatggca gaacgtgcag tgcagctggg cggtgtagct ctggggacca
      541 ctcaagttat caacagcaaa accccgctga aaagttaccc gctggacatc cacaacgttc
      601 aggatcacct gaaagaactg gctgaccgtt acgcaatcgt cgctaatgac gtacgcaaag
      661 cgattggcga agcgaaagat gacgacaccg cagatatcct gaccgccgcg tctcgcgacc
      721 tggataaatt cctgtggttt atcgagtcta acatcgaata aatccatcgc tgatggtgca
      781 gaactttagt acccgataaa agcggcttcc tgacaggagg ccgttttgtt ttgcagccca
      841 cctcaacgca cttatttagt gcatccatct gctatctcca gctgattaag taaatttttt
      901 gtatccacat catcacacaa tcgttacata aagattgttt tttcatcagg ttttacgcta
      961 aataatcact gtgttgagtg cacaatttta gcgcaccaga ttggtgcccc agaatggtgc
     1021 atcttcaggg tattgcccta taaatcgtgc atcacgtttt tgccgcatct cgaaaaatca
     1081 aggagttgca aaactggcac gattttttca tatatgtgaa tgtcacgcag gggatcgtcc
     1141 cgtggataga aaaaaggaaa tgctatgaag tctgtattaa aagtttcact ggctgcactg
     1201 accctggctt ttgcggtttc ttctcatgcc gcggataaaa aattagttgt cgcgacggat
     1261 accgccttcg ttccgtttga atttaaacag ggcgataaat atgtgggctt tgacgttgat
     1321 ctgtgggctg ccatcgctaa agagctgaag ctggattacg aactgaagcc gatggatttc
     1381 agtgggatca ttccggcact gcaaaccaaa aacgtcgatc tggcgctggc gggcattacc
     1441 atcaccgacg agcgtaaaaa agcgatcgat ttctctgacg gctactacaa aagcggcctg
     1501 ttagtgatgg tgaaagctaa caataacgat gtgaaaagcg tgaaagatct cgacgggaaa
     1561 gtggttgctg ttaagagcgg tactggctcc gttgattacg cgaaagcaaa catcaaaact
     1621 aaagatctgc gtcagttccc gaacatcgat aacgcctata tggaactggg caccaaccgc
     1681 gcagacgccg ttctgcacga tacgccaaac attctgtact tcatcaaaac cgccggtaac
     1741 ggtcagttca aagcggtagg tgactctctg gaagcgcagc aatacggtat tgcgttcccg
     1801 aaaggtagcg acgagctgcg tgacaaagtc aacggcgcgt tgaaaaccct gcgcgagaac
     1861 ggaacttaca acgaaatcta caaaaaatgg ttcggtactg aaccgaaata ataacgctac
     1921 acctgtaaag cgcactggca gttccctctc ccctctgggg agaggattag ggtgaggggc
     1981 gcaaacccgc tccggggcca ttaattaccc tgaatttgat tatttacacc acggtaacag
     2041 gaacaacata tgcagtttga ctggagtgcc atctggcctg ccattccgct tctgattgaa
     2101 ggtgccaaaa tgaccctgtg gatttcggtc ctcggtctgg ctggcggtct ggtaatcgga
     2161 ttgctggcag gttttgcacg caccttcggt ggttggatag ccaaccacgt cgcgctggtc
     2221 tttattgagg tgatccgcgg cacacctatc gtcgtccagg tgatgtttat ttatttcgcc
     2281 ctgccgatgg cgtttaacga cttacgcatc gacccattta ctgcggcggt ggtcaccatc
     2341 atgatcaact ccggcgcgta cattgcggaa atcacgcgtg gtgcggtgct gtctattcac
     2401 aaaggttttc gtgaagcagg actggcgctc ggtctttcac gttgggaaac cattcgctac
     2461 gtcattttac cgctggcact gcgtcgtatg ctgccgccgc tgggtaacca gtggatcatc
     2521 agcattaaag acacctcgct gtttattgtg atcggcgtgg cggaactgac ccgtcagggg
     2581 caagaaatta ttgccggtaa cttccgcgcc cttgagatct ggagcgccgt ggcggtgttc
     2641 tatctgatta ttaccctggt gctaagcttt attttgcgtc gtctggaaag aaggatgaaa
     2701 atcctgtgat tgaatttaaa aacgtctcca agcactttgg cccaacccag gtgcttcaca
     2761 atatcgattt gaacattgcc cagggcgaag tcgtggtgat tatcgggccg tccggttccg
     2821 gtaaatcgac cctgctgcgc tgcatcaaca aactggaaga aatcacctcc ggcgatctga
     2881 ttgtcgatgg cctgaaggtt aacgatccga aagttgacga gcgcctgatt cgccaggaag
     2941 caggtatggt gttccagcag ttttacctct tcccgcatct gacggcgctg gaaaacgtca
     3001 tgtttggtcc gctacgcgtg cgtggcgcga acaaagaaga ggcggaaaaa ctggcgcgtg
     3061 agctgctggc gaaagtcggt ctggcagaac gtgcacatca ctacccttcc gaactttctg
     3121 gtggtcaaca gcagcgtgtg gcgattgccc gcgcgctggc ggtgaagccg aaaatgatgc
     3181 tgtttgatga accgacttcc gctcttgacc cggaactgcg ccatgaagtg ctgaaggtta
     3241 tgcaggatct ggctgaagaa gggatgacga tggtgatcgt gacccacgaa atcggttttg
     3301 ccgagaaagt agcttcgcgg ctgatcttta tcgacaaagg ccggattgcg gaagatggcg
     3361 atccgcaggt attgatcaag aacccgccga gccagcgctt gcaggaattt ttgcagcacg
     3421 tctcttaact tctctgcctc cctttcaccc gaaagggagg cacaccagat tcctctcatt
     3481 taaaatcgcc cctcccccag catctatact tatctttttg ctctgttttc tcactggagg
     3541 agtcatgcgg tggatcctgt tcatcctctt ctgcctgctg ggcgcacctg cccacgcggt
     3601 atccataccc ggcgttacaa ccacaacgac aacggactca acgactgaac cggccccgga
     3661 accggatatc gaacaaaaaa aagcggccta tggcgcactg gcggatgtgc tggataacga
     3721 cacctcgcgt aaagaattga tcgaccagtt gcgtactgtc gccgcgacgc cccctgcgga
     3781 accggtcccg aagatcgtgc cgccgacgct ggttgaagag caaaccgtgc tgcaaaaggt
     3841 caccgaagtc agccgccatt atggtgaagc cctttccgcc cgcttcgggc aactttatcg
     3901 caatatcacc ggctccccgc ataagccatt taatccacaa accttcagca atgcgctgag
     3961 ccatttttca atgttagcgg tattagtgtt tggtttttac tggctgattc gcctgtgcgc
     4021 actgccgctg tatcgcaaaa tgggtcagtg ggcgaggcaa aaaaatcgtg agcgcagcaa
     4081 ctggttgcag cttccggcga tgattatcgg ggcgtttatt atcgacctgc tgttactggc
     4141 cctgacatta tttgtcggcc aggtattaag cgacaacctg aatgcaggca gtcgcaccat
     4201 cgctttccaa caaagtttgt ttctcaacgc ctttgccctg attgaatttt tcaaagccgt
     4261 actacgcctg attttttgcc caaacgtggc ggagctgcgc ccgttcacga ttcaggacga
     4321 gagcgcccgt tactggagtc gtcgcctgag ctggttaagc agtttgatag gttatggcct
     4381 gattgtggcc gtgccgatta tctctaatca ggtgaatgta cagataggtg cgctggcgaa
     4441 cgtcatcatt atgctgtgca tgaccgtctg ggcgttgtac ctgatctttc gtaataaaaa
     4501 agagattacc cagcatttgc tcaactttgc ggagcattcg ctggcctttt tcagcctgtt
     4561 tatccgcgcc tttgcgctgg tgtggcactg gctggcaagc gcctatttta tcgtgctgtt
     4621 tttcttttcg ttgttcgatc cgggcaacag cctgaaattt atgatgggtg caacggtgcg
     4681 cagcctggcg attattggta tcgcagcgtt tgtttccggt atgttttccc gctggctggc
     4741 aaaaaccatc actctctcgc cacatactca gcgtaactat ccagagttgc aaaaacggct
     4801 gaatggctgg ctttcggcgg cgctgaaaac ggcgcgtatt ctgaccgtct gcgtggcggt
     4861 aatgttgctg ttgagcgcat ggggattgtt cgatttctgg aactggctgc aaaacggcgc
     4921 ggggcagaaa accgtagata tcctgatccg tatcgcactt attctttttt tctcggcggt
     4981 tggctggacg gtgctcgcca gtttgatcga aaaccggctg gcttcggata ttcatggccg
     5041 cccgctaccc agcgcccgta cgcgtaccct gctgacgctg ttccgtaacg cgctggcggt
     5101 gattatcagt accatcacca tcatgattgt gttgtcggaa atcggcgtca atatcgcgcc
     5161 attgctggca ggtgccgggg cattaggtct ggctatctcg tttggttcgc aaacgctggt
     5221 gaaagatatc attaccgggg tgtttattca gtttgaaaac ggcatgaaca ctggagattt
     5281 ggtgactatc gggccgttga ccggcacagt ggaacggatg tcgattcgct ccgtgggcgt
     5341 gcgccaggat accggggcgt atcacatcat tccgtggtct tcgataacca cctttgctaa
     5401 cttcgtccgc ggtattggtt cggtagtggc aaactacgat gttgatcgcc atgaagatgc
     5461 tgataaagcc aatcaggcac tgaaagatgc ggtagcggaa ttaatggaaa acgaagaaat
     5521 tcgcgggctg attattggtg aaccgaattt tgccgggatt gtcggcttaa gcaataccgc
     5581 gtttacactg cgtgtttcgt tcaccacgct gccactcaaa cagtggacgg tacgctttgc
     5641 cctcgacagc caggtgaaaa aacatttcga cctggcgggc gttcgcgcgc cagtgcagac
     5701 ttatcaggtg ctgcctactc cgggcgcgac cccggctgaa ccgttaccgc cgggggagcc
     5761 aacgctttaa cgctggcgat tgacaaaacg gcggcgctgc tcgtcgttca taaaggtcca
     5821 ggcaataaag cgactctgct tttgcccctg ggccatctct ttttttacca ccttcaccgc
     5881 gcccacgtcg gtcagagcac gatacaacgg cggtaagttt tcaccacgag ataccagcga
     5941 ggtaaaccac atcacctgct tcgcgaagcc tttgctctct tcaatcatct ttttgataaa
     6001 ggcgacttca ccaccttcac accacaactc ctgttgctga ccaccaaagt tcagtgcatc
     6061 gtctttgttc agccccaggt tacggcgttt acgctcacta cctgcccgcg ctgcagcggc
     6121 ggaatcgtgg aatggcgggt tacacaaggt cgcgtcgtat tgctcgtttt tatggatgat
     6181 gccgttaaaa attgccccac tctctttttg ccgacgcaga cgaatggcgc ggttaagccc
     6241 cggattagca ctgataatcg cctgcgcact gcttaacgcc tggctgctgg tttcactacc
     6301 ggtaaaacgc cagccatatt catgtacgcc aatcagcgga taaatacagt tcgcaccaac
     6361 gccgatatcc agaatgctgg cattcgctgg aattgttccg cttgcttcag ccagtaaatc
     6421 ggcaaggtga tgaatataat ccgcccggcc cggtactggt gggcagagaa aaccgtcggg
     6481 gatatcccag ttcgctacgg cgtaaaaatg ggccagcaac gccttattga gcgccttcac
     6541 cgccagcgga ttggcaaagt ctacgctttg ctccccggcg ggtgtaagcg tgaggaattg
     6601 cctgagttca ggattgacct gacaaagcgt ggcgagatcg tagcggctgt gatgacggtt
     6661 gcgcggatgc aaccccggtt tctgggcgga catagcattc tcctttaagc agcggcgtaa
     6721 gatacccgca taacgcgaca gggtaaatat ctggctgcgt ttatcattcc ccaaaacctt
     6781 aacatggcta tggtttatgg caattcatca taaaaatcag cattatgcat gtcattaatt
     6841 tgtcttattt tgtaaaaata tatcgttcaa aaaataagca catctctgtc aattttttgc
     6901 gtcatttgca tcgcgcaggc gacctacact tatcaggcac tacccgttgt atttatgagg
     6961 atgtcattat gaaaaagtgc ctcacactac tgattgccac cgtcctgagc ggaatctctc
     7021 tcacggctta tgccgcgcaa ccgatgagta acctggacag cggtcagcta cggcccgccg
     7081 gtaccgtttc ggcaactggc gcatcaaacc taagcgatct ggaggataaa ctggcagaaa
     7141 aagcgcgcga acaaggcgcg aaaggttatg tcattaattc cgcaggcgga aatgaccaga
     7201 tgttcggtac tgcaaccatc tacaaataac cccaccaacg taactgcatt cgcctctgga
     7261 tgcagttttc gcctgttttg taatcaaaaa acatcaaata tgaactcaat gtaaataaat
     7321 gtatttcttt ttcgcgcaat gggtgataga aaatcgcgcc aagtgataat gcttatcaaa
     7381 attattatca cttgcatgaa cactatcacg ggattaacag tggcatcgca tccgcagaga
     7441 tgctttctcg tggcagtgaa aatttcaaca tataagaaaa agtcacctgc aaaatggaaa
     7501 acaatcgcaa tttccctgcc agacaatttc attcgctcac gttctttgcc ggtctttgta
     7561 ttggcatcac gcctgtggct caggcactcg ccgccgaagg gcaaactaac gcggatgaca
     7621 cgctggttgt cgaagcatca acgccttcgc tttatgcgcc acaacaatct gccgatccga
     7681 aattctcgcg tccggtagcg gatactaccc gcacgatgac ggtgatttct gaacaagtga
     7741 ttaaagatca gggcgcaacc aaccttaccg acgcgctcaa aaacgccccc ggcg
//
LOCUS       DAEEKQ010000181         7777 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.183, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000181 DAEEKQ010000000
VERSION     DAEEKQ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7777)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7777)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7777
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.183"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1780)
                     /locus_tag="KLK57_003603"
     CDS             complement(<1..1780)
                     /locus_tag="KLK57_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HBE0893874.1"
                     /translation="MEKHGAELLLQRMLSNTSATFREGQWEAIDAVVNQRRKLLVVQR
                     TGWGKSAVYFIASKIFRDRGAGPTIIISPLLALMRNQVAAAERLGITAETLNSTNREE
                     WQRISDKLLRGGVDCLLISPERLANQDFLETANTPVLGTTATANNRVVEDIRQQLGDI
                     VIQRGTLARESLALDALVLGEQSSRLAWLATVIPQFSKSGIVYTLTTRDAELVAEWLR
                     TNGISAFAYYSGVTCEGAEDSNTAREYLEQALLANKIKVLVATTALGMGFDKPDLGFV
                     IHYQMPGSIVGYYQQVGRAGRAIDSAVGILLCGGEDRAIHKFFRESAFPAEAQIHEIL
                     NVLSENDGLTLRGIEQRTNLRYGQIEKALKLLVAENPSPVVYTEKLWRRTIVSFSPDH
                     ERINHLMNQRKNELADVESYITTKECKMQFLRRALDEPSAERCGKCSSCLQHPLLSPD
                     IDSGLLHAANLFIKHADLPLNLNKQVASGAFTQYGFKGNLPAGLQGSTGRVLSRWGDS
                     GWGKQVAQEKKTGRFSDELVEACAEMVRQRWNPHPEPTWVCCVPSLKHLDLVPDFARR
                     LAAKLGLPFIDAIEKVVDNPPQKMQQN"
     gene            complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="KLK57_003604"
     CDS             complement(1921..3261)
                     /gene="gntU"
                     /locus_tag="KLK57_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HBE0893875.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTDTNLVKLIIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDINEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNNSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="KLK57_003605"
     CDS             complement(3265..3792)
                     /gene="gntK"
                     /locus_tag="KLK57_003605"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HBE0893876.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="KLK57_003606"
     CDS             complement(3931..4926)
                     /gene="gntR"
                     /locus_tag="KLK57_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HBE0893877.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="KLK57_003607"
     CDS             complement(5150..5845)
                     /gene="yhhW"
                     /locus_tag="KLK57_003607"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HBE0893878.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(5968..7005)
                     /locus_tag="KLK57_003608"
     CDS             complement(5968..7005)
                     /locus_tag="KLK57_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HBE0893879.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYILNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRVLEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDICSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            7339..>7777
                     /gene="yhhY"
                     /locus_tag="KLK57_003609"
     CDS             7339..>7777
                     /gene="yhhY"
                     /locus_tag="KLK57_003609"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HBE0893880.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQHPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYA"
BASE COUNT         1978 a         2101 c         1871 g         1827 t
ORIGIN      
        1 ggttttgctg cattttctgc ggtggattgt ccacgacttt ttcaatggca tcaataaaag
       61 gtaagccaag tttcgccgcc agtcgccggg caaaatcagg aaccaggtcg aggtgcttta
      121 atgaaggaac gcagcatacc caggttggtt caggatgcgg attccagcgt tggcgaacca
      181 tttccgcaca tgcctccacc agctcatcac tgaatcgccc cgtttttttc tcctgtgcta
      241 cctgctttcc ccacccggaa tctccccaac gagaaaggac tcgccccgta gatccttgta
      301 aacccgcagg caagttccct ttaaatccgt attgcgtaaa agctccagat gctacctgtt
      361 tattgagatt taatggcagg tcagcgtgtt taataaataa atttgccgca tggagtaagc
      421 cgctatcaat atcgggcgac aacaacggat gctgaagaca actgctacat ttaccacaac
      481 gttcggcact tggctcatcg agtgcgcggc gcagaaattg cattttgcac tccttggtcg
      541 tgatatagct ttcaacatct gccagctcat tttttctctg attcatcaaa tggttaattc
      601 gttcatggtc aggagagaaa ctgacgatag ttctgcgcca taatttctcg gtatacacca
      661 caggcgatgg attttcagct accaataatt tcagtgcttt ttctatttgc ccataacgga
      721 gattcgtccg ttgttcaatg cctcgtaggg taagaccgtc attctcgcta agtacgttga
      781 gtatttcatg aatttgcgcc tccgcaggaa aggcgctttc acggaagaat ttatgaatag
      841 cgcggtcttc accaccacaa agcaatatgc caactgcgga atctatagca cgcccggcac
      901 gccctacctg ttggtagtaa ccgacaatag accccggcat ttgataatga atgacgaaac
      961 ctaaatcagg tttatcaaac cccataccta atgccgtcgt cgcgaccagc actttgattt
     1021 tatttgccag cagcgcctgc tccagatatt ccctggcagt attagaatct tccgcgcctt
     1081 cacaagtcac gccgctgtag taagcaaatg cactgatacc atttgtcctt aaccactcgg
     1141 cgacaagttc ggcatcgcga gtagtcaggg tataaacaat tcccgatttg gaaaactgag
     1201 ggataaccgt tgctaaccat gccagacggg atgactgttc acctaagact aaggcatcaa
     1261 gcgccaaact ttcgcgagcc agcgttccac gctgaatcac aatgtcaccc aattgctgac
     1321 ggatatcctc aacgacacga ttattcgctg tcgcggttgt acccagaaca ggggtattcg
     1381 cagtttcaag aaaatcctga ttcgccaacc gttcaggtga aatcagcaga caatctacac
     1441 caccccgcag caacttatcg ctaatgcgct gccattcttc cctgtttgta gaattcaacg
     1501 tttcagccgt aatacctaac cgttcagcgg cggcaacctg gttacgcatc agggcaagca
     1561 aaggagagat gataattgtc gggccagcgc cgcgatcgcg gaagatttta ctggcgatga
     1621 agtacacggc acttttcccc cagccagtgc gttggacaac cagcagtttt cgccgctgat
     1681 taaccaccgc atctatagct tcccattgcc cttccctgaa agtagcgctg gtgttactta
     1741 acatccgctg taacagcaat tcagctccgt gtttctccat catcttctcc ctcgtccgtt
     1801 aacgcagtga ttagaacgga taatgttccg gtctttttgc gagagagatc aaaaagatga
     1861 agacaggaga taagagaaat gaattactgc gagcaagctc gcagtaattt gtcgcagtaa
     1921 ttaactcaac agctggaacg caatcatccc aacaatggca ccaacagtgc cgaggatggt
     1981 ttccatcatg gtccaggttt tcagcgtttc ggcttcggtc gcgccggtaa atttaccgaa
     2041 caaccagaaa ccagcgtcgt taacgtggct gacaacaatc gaaccaccgg cgatacaaat
     2101 cgacagcgcc gccatttgcg caccggaatt gttcagttgt tcaatgaccg gcatcaccag
     2161 ccccaccgcc gttaaacagg caacggtggc agacccctga atgatgcgca ctgcagctgc
     2221 cagcacgaag caggtgatag caatcggcag gcccatgccg gttaacgctt cgcccagtgc
     2281 cggacctacg ccagagtcaa ccagcacctg tttgaacacg ccgcctgcac caatcaccag
     2341 cagaatgatc cccgccggtt gcagcgcgtg accgcaaatc tccatcactt tgtcttttgg
     2401 catgccctga cgcattgcca ggccataaat cgctaccaga caagcaacca gaatcgcggt
     2461 aaacggatga ccaataaact cgaaccattc gtaagcggta gagccttctg gcacaaaacg
     2521 cgcggcaatg gttttcagcc ccaccagcac cagcggcaac aggatcagcg acaggctgaa
     2581 tccgaaagat ggcattttac cttcgccgag atgcggctcg ttgatgtcgt caggaatatg
     2641 cagctcaacg taacggctga tgaagttacc ccacagcggc ccggcaataa tcattcccgg
     2701 aattgccgca cacaggccaa tcaggatcat ccagccaaaa tcggcgttca tctgcgatgc
     2761 cagcagcatt ggcgctggcc ctggcaccag gaacgccgcc gctgccgcca cgcctgcgaa
     2821 taatgggatt atcagcttca ccaggttcgt atcggtgtgg cgcgccattg agaaagcaac
     2881 gctaatcagc agaacaatcg ccacttcaaa gaacagcggt agcgcacaga ccagccccgc
     2941 aaggccgatg gcataatgcg cgcggctgtg accgaaggat ttgagcattt tgacggcaat
     3001 ctgatcgact gcgccggttt catgtaagat cttgccaaac atagctccca gggcgacaac
     3061 caccgccagg aagccgaggg tgcctcccat ccctttttcc atcgtcgctg cgattttatc
     3121 gagcggcata ccggaaaaaa ggccagcccc catggacacc accatcaaag ccaggaaagc
     3181 gtgcatacgc gccttcatga ctaaaaacag cagcagtaaa acagacccta ctgctgttaa
     3241 aacaagcgtt aatgtagtca ctacttattt gcctttttta ataacctcaa tggtgcttgc
     3301 cacaacacct tccagcggtt gatcgatatc caccaccagt acatcggttt cgtccgcacc
     3361 cggctcctgc agcgtttcaa actgcgtcac caacatttgg gttttaaaga aatggccttt
     3421 gcgcgctttc aggcggcttt caatcacgtc aaaatcgcct ttcaggtaga tgaaagagag
     3481 attcggatta ccttcacgca gcaagtcgcg atagtgtttt ttcaatgcag aacagacgat
     3541 cagtgacact ttattagtgc gctgcatagc aaacgcggcg tcgttcagcg cctgcaacca
     3601 gggtttgcga tcgtcgtcat tcagcggttc gccagacgcc attttttcga tattgcgccg
     3661 tggatggagg aaatcgccat caagaaacgc ggcatgaagt tgatgcgcca cttcactggc
     3721 gaccgcagat ttgccgctgc ccgatacgcc catcaagacg taaatgtggt gatcatggtt
     3781 agtcgtgctc aaagtggtgc ccccacaata caagaattaa aaatgttacg ggtaactgtt
     3841 atcggtaaca ttgtccagcc ggacaatatc agaagcaata tccatccgtg ccttaagtgt
     3901 ataagtgtga gctacttcaa atttgtaggc ttaaatagat ccgcccggtg acaaggtgaa
     3961 acctaaatct aacattttcg gtgtcacaga ttcgccacga atacgcgcca gcaggcgttc
     4021 agcgccaata ctgcccatcc gctcacgcgg cgtcagcacg ctcgctaacc gcggctccat
     4081 cacctggcca atgtcatgac cgtggaaacc ggcaatcgcc atatcgtcag gaaccttcaa
     4141 tcccagacgc tgacattcaa acgccgcgcc gaccgccagg tcatcgttgg tacagaacac
     4201 gccatccagc tgcggatatt cccgccgcgc ctggcgaatc agttcaatac cggaagagta
     4261 agaagaagat tgctcaacca tcacgctata cggcaccaga cctgcatcca gcatcgcctg
     4321 ttcgtatccc ttctgtttga tgatagtacg ttcgtcgaga cgcgcgccga gataggcaat
     4381 gtggcaatgc ccgcgagcaa taatggcggt ggtcatctgg cgtgccgctt caaagttatc
     4441 aaaaccgacg gcgatatcga ggcacggcga ctggctgtcc atgagttcca ccaccgggat
     4501 ccccgccact tcaatcatct ttaaggtgcg cggcgtgtgg gtacgttcgg tgaggatcag
     4561 tccgtcgata ttccaggaga gcatggattc gagacgttct tgctccattt ccggtttata
     4621 accgtagtga gccagcatgg tctgataacc gtgcgcgtcg gtgacgcttt cgattccgcg
     4681 taatacttcc gcgaaaacct ggttggtgag agaaggtaac aggacgccaa tcgcccggct
     4741 ggtggcgtta gagaggatat cgggcgcacg attgggaata tagcccagtt catcaagagc
     4801 ggcggcaatc ttgccgcgta gagcgacgga aacctgctcc gggttgcgta aaaaacggct
     4861 gaccgtcatt ttggtcacgc ctacacggtc agccacatcc tgaagtacgg gtcttttctt
     4921 tttcatcgtc ctgaaggtac aaaagagata gattctctta gtttaacacg gacgcatcag
     4981 aaccttcccg gggaaaacag tgcttgttta caattattag atatacacta aatcaattgc
     5041 atcgcattgt gctaacgacg cggtaaaacc ccggaagcgt gcatcagcac gcgaccggag
     5101 cgagcgagga agcctgcaaa aaggcaggct tcaaagatgc cgttgagttt taaaccggcg
     5161 gcagatcgaa cagtaacact tcgctgtcgc tatccgcatg gatggagatt gcctgctcat
     5221 cccagattgc cagaccatcg ctggtcgagg ctttcacgcc gttaatggtg acattgcctt
     5281 tcaccacctg gatccagacg cggcgttcag cggcaatctg atgcaccgac tgctcatctt
     5341 tcagcaacgc ccagcggtac agttccatat cctgatgcac tttcaatgaa ccatctcgcg
     5401 catccggcga gagcaccagc tgtttgccct gtacggcatc gaagcgacgc tgttcataac
     5461 gcggcgtaat accgttttct tcaggcatga tccagatctg atacagatgc agacgctcgg
     5521 tgctgcttgg gttgtactct gagtgacgaa tacccgtacc cgcactcata atctggaact
     5581 cacccgccgg aacctgctct ttattgccca tgctgtcctg atgctcaaca gtaccttcca
     5641 gcacgtaggt caaaatttcc atatctttat gcgggtgagt gccgaagccc tgccctgctt
     5701 caatcacgtc gtcgttaatc acgcgcagcg cggagaagcc cataaagttc ggatcgtaat
     5761 agttggcaaa agagaaagta tgccaggagt ccagccagcc atgatttgca tgaccacgtt
     5821 catttgcttt gcgtaagtag atcatttgta ttcgccccat gaatgatttc gatgggctta
     5881 gtgtggaccc gatctgcctg ggatgataga gggtgaaaat tgacccctct gttcaaaaaa
     5941 attgaactat tcgaggggcc atcaaactta cttcgcgaga gtcactgtgg agggagaggc
     6001 ttgctcaaat ccgcgttcaa ggatttccag attggtaaga acttcagatt ccttgacgta
     6061 atttggcgca ccgtgggtga tggtttgata caacgcatca taaacgcgcc cgtaatcgcc
     6121 catctccggc ttcatctctt ctctgaccgt cacgccctcg tcattgacat actccagcac
     6181 accgaccgaa tcatccgctg cgaatcccgg ttcgcccggc ataatattag ccttcaggct
     6241 ggtttcctgc tggtcgatac cgtatttaat aaacgaacct ttcttaccgt gaacgataaa
     6301 tttcggataa tcgattttca ccagatggct ggttttgacg atggctttca ggtcgccata
     6361 aaacagttgc gcttcaaagg tgtcgtcagg attggcttta ttacgcaggc tgcagatgtc
     6421 ataagcgacg tgatccgggc gaccgaacag agaaataatc tggtccatcg tatgcacacc
     6481 aaggccatag aacgcgccat cctgcggcaa cccaggtttg gtttctgcca ccgggcggta
     6541 atagtcaaaa tggctttcca cttcaacaat ctctcccagc ttaccgcttt caatcgcttt
     6601 tttcgccgtc aggaagcagg aatcaaagcg acgattctga tatggcgtga cggtcagccc
     6661 tttgcttttc gccagtgcaa acagctcttt cgcctgcgca agtgtcggag tgaacggttt
     6721 ttcgaccagc acatttttcc cggcttccag cacgcgcttc gcgtactcga agtggctgtc
     6781 cgcatgggtg cagacaacaa ccaacttaac atcgggatcg tttagcactt cgtcgagatc
     6841 gctggtgaaa tggatatgag agtaaatggg agcctgttct tccggcttcg catggcgacg
     6901 aaaaatatgc gcgacatgcc agctatcctt gcggttaagt atatacggca gatggtaacg
     6961 ggtggtgctt ttgccgaagc caataaaggc gcagttgatg accatgattt cgtccttttt
     7021 aaggtggtta tttacacctt agcgcaaagc ggacgtggtt cctactggag tgcgcataac
     7081 gaacacaagc actcccgtgg ataaattgag aacgaaagat caaaaaaaaa gccagcaccc
     7141 ggctggctaa gtaatactgg aagcaatgtg agcaatgtcg tgctttcagg ttctccgcga
     7201 gggtcttcct gatcgcgaga caataataat cattctcatt cgcacttgtc caacactttt
     7261 tgcaaaaaaa atgcatttga ctcgcatttg aaagtcaatg atgttgaaag ggacatttac
     7321 cccaaagagg acaaaggaat gagtgagata gtaatacgcc acgcagaaac acgggattac
     7381 gaggccatca ggcagattca cgcccagccg gaggtgtatt gcaacacact acaggtgcct
     7441 catccttccg atcatatgtg gcaggagcga ctcgccgatc gtcccggcat caagcaactc
     7501 gtcgcctgta ttgatggaga cgtcgtgggc catctcacca ttgacgtgca acagcaccca
     7561 cgccgcagtc atgttgccga ttttggtatc tgtgtcgact ctcgttggaa gaaccgcggc
     7621 gtcgccagcg ccctgatgcg agagatgatt gaaatgtgcg acaactggtt gcgggtagat
     7681 cgcattgaac taaccgtgtt tgtcgataac gccccggcaa ttaaggtcta taaaaaatac
     7741 ggctttgaaa ttgaagggac tggtaagaag tacgcat
//
LOCUS       DAEEKQ010000182         7715 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.184, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000182 DAEEKQ010000000
VERSION     DAEEKQ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7715
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.184"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..643
                     /locus_tag="KLK57_003610"
     CDS             <1..643
                     /locus_tag="KLK57_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0893881.1"
                     /translation="HAFITESDADGHVLTVREDLIWPLLEVLYPGTRETFGLPGICLS
                     LADKPDELAALEHYWQLIERESVEQLPGREHTLTLLAQAVFTLLLRNAKLDDHAASGM
                     RGELKLFQRFNMLIESHFHQHWTVPDYANELHITESRLTDICRRFANRPPKRLIFDRQ
                     LREAKRLLLFSDNAVNNIAWQLGFKDPAYFARFFNRLVGCSPSAYRAKKVPVT"
     gene            894..2456
                     /gene="hpaB"
                     /locus_tag="KLK57_003611"
     CDS             894..2456
                     /gene="hpaB"
                     /locus_tag="KLK57_003611"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HBE0893882.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDANGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHATLQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            2474..2986
                     /locus_tag="KLK57_003612"
     CDS             2474..2986
                     /locus_tag="KLK57_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HBE0893883.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            3379..5529
                     /gene="btsT"
                     /locus_tag="KLK57_003613"
     CDS             3379..5529
                     /gene="btsT"
                     /locus_tag="KLK57_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HBE0893884.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGHLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            5579..5782
                     /locus_tag="KLK57_003614"
     CDS             5579..5782
                     /locus_tag="KLK57_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HBE0893885.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            5793..6749
                     /gene="yjiA"
                     /locus_tag="KLK57_003615"
     CDS             5793..6749
                     /gene="yjiA"
                     /locus_tag="KLK57_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HBE0893886.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYEIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEDEIRAAFAGLKK"
     gene            complement(6827..>7715)
                     /locus_tag="KLK57_003616"
     CDS             complement(6827..>7715)
                     /locus_tag="KLK57_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HBE0893887.1"
                     /translation="RVTEIDRLLEQKPRLTADQAWDVIRQTSRQDLNLRLFLPTLQAA
                     TSGLTQSDPRRQLVDTLTRWDGINLLNDDGKTWQQPGSAILNVWLTSMLKRTVVAAVP
                     MPFDKWYSASGYETTQDGPTGSLNISVGAKILYEAVQGDKSPIPQAVDLFAGKPQQEV
                     VLAALEDTWETLSKRYGNNVSNWKTPAMALTFRANNFFGVPQAAAEETRHQAEYQNRG
                     TENDMIVFSPTTSDRPVLAWDVVAPGQSGFIAPDGTVDQHYEDQLKMYENFGRKSLWL
                     TKQDVEAHKESQEVLHVQR"
BASE COUNT         1784 a         2101 c         2021 g         1809 t
ORIGIN      
        1 tcatgcgttt attacggagt ctgacgccga cggtcatgtg ttgacggtac gggaagatct
       61 gatctggccc ctgctggaag ttctttatcc aggcactcgg gaaaccttcg gcctgccggg
      121 gatttgcctg tcactggcag ataaacccga cgaactggcg gcgctggaac actattggca
      181 actgatagag cgggaatcgg tggaacaact gcctggacgg gaacacaccc tgacgctact
      241 ggcacaggca gtgttcaccc tactgctgcg taatgcaaaa ctcgacgacc atgccgccag
      301 cgggatgcgc ggagaattaa aactgttcca gcgttttaat atgctgattg aaagccattt
      361 tcatcagcac tggacagtac cggattacgc taacgaactg catatcaccg aatcacgcct
      421 cacggacatc tgccgccgct ttgccaaccg tccgccaaaa cggttgattt ttgacaggca
      481 gctgcgagaa gccaagcggc tgctgctgtt ttctgataac gccgtgaaca atattgcctg
      541 gcaactcggt tttaaggatc cagcttattt tgcgcgcttt tttaatcgct tagtcggttg
      601 ctcgcccagt gcttatcgtg ccaaaaaagt acctgtgacg tgaaaatccc ttagccttaa
      661 cgggaaacca ggcaccacct gctattcccc tttctccgcc tggagcaagg ggatttagta
      721 acgtcaaaaa tcgctaaaag cgaccgcgat cacaaaaccg tacccgacct gaaaagtacc
      781 agccattgtc ccaaaagtgt cttcccgtaa tcgcccctgc aagcttcaat tgaataacaa
      841 aaaaaacaca caaaattaac aatcgtattc cgattaatac tgtagaggtc gacatgaaac
      901 cagaagattt ccgcgccagt acccaacgtc cgttcaccgg ggaagagtat ctgaaaagcc
      961 tgcaggatgg tcgcgagatc tatatctatg gcgagcgagt gaaagacgtc actactcatc
     1021 cggcatttcg taatgcggca gcgtctgttg cccaactgta cgacgcgctg cacaaaccgg
     1081 agatgcagga ctctctgtgc tggaacaccg acaccggcag cggcggctat acccataaat
     1141 tcttccgcgt ggcgaaaagt gccgacgacc tgcgccagca acgtgacgcc atcgctgagt
     1201 ggtcacgcct gagctatggc tggatgggcc gtaccccaga ctacaaagct gctttcggtt
     1261 gcgcactggg cgcgaatccg ggcttttacg gtcagttcga gcagaacgcc cgtaactggt
     1321 acacccgtat tcaggaaact ggcctctact ttaaccacgc gattgttaac ccaccgatcg
     1381 atcgtcattt gccgaccgat aaagtgaaag acgtttacat caagctggaa aaagagactg
     1441 acgccgggat tatcgttagc ggtgcgaaag tggttgccac caactcggcg ctgactcact
     1501 acaacatgat tggcttcggc tcggcacaag taatgggcga aaacccggac ttcgcactga
     1561 tgttcgttgc gccaatggat gccaatggcg tcaaattaat ctcccgcgcc tcttatgaga
     1621 tggtcgcggg tgctaccggc tcgccgtacg actacccgct ctccagccgc ttcgatgaga
     1681 acgatgcgat tctggtgatg gataacgtgc tgatcccatg ggaaaacgta ctgatctacc
     1741 gcgatttcga tcgctgccgt cgctggacaa tggaaggcgg ttttgcccgt atgtatccgc
     1801 tgcaagcctg tgtgcgcctg gcagtgaaac tcgacttcat tacagcactg ctgaaaaaat
     1861 cactcgaatg taccggcacc ctggagttcc gtggtgtaca ggccgatctc ggtgaagtgg
     1921 tggcgtggcg caacaccttc tgggcattga gtgactcgat gtgttctgaa gcgacgccgt
     1981 gggtcaacgg ggcttattta ccggatcatg ccacactgca aacctatcgc gtactggcac
     2041 caatggccta cgcgaagatc aaaaacatta tcgaacgcaa cgttaccagt ggcctgatct
     2101 atctcccttc cagtgcccgt gacctgaaca atccgcagat cgaccagtat ctggcgaagt
     2161 atgtgcgcgg ttcgaatggt atggatcacg tccagcgcat caagatcctc aaactgatgt
     2221 gggacgccat tggcagcgag tttggtggtc gccacgaact gtatgaaatc aactactccg
     2281 gtagccagga tgagattcgc ctacagtgtc tgcgccaggc acaaagctcc ggcaatatgg
     2341 acaagatgat ggcgatggtt gatcgctgcc tgtcggaata cgaccagaac ggctggactg
     2401 tgccgcactt gcacaacaac gacgatatca acatgctgga taagctgctg aaataacgca
     2461 gcaggaggtt aagatgcaat tagatgaaca gcgcctgcgc tttcgtgacg cgatggccag
     2521 cctgtcggca gcggtaaata ttatcaccac cgagggcgac gccggacaat gcgggattac
     2581 ggcaacggcc gtctgctcgg tcacggatac accaccatcg ctgatggtgt gcattaacgc
     2641 caacagtgcg atgaacccgg tttttcaggg caacggtaag ttgtgcgtca acgtcctcaa
     2701 ccatgagcag gaactgatgg cacgccactt cgcgggcatg acaggcatgg cgatggaaga
     2761 gcgttttagc ctctcatgct ggcaaaaagg tccgctggcg cagccggtgc tgaaaggttc
     2821 gctggccagt cttgaaggtg agatccgcga tgtgcaggca attggcacac atctggtgta
     2881 tctggtggag attaaaaaca tcatcctcag tgcagaaggt cacggactta tctactttaa
     2941 acgccgtttc catccggtga tgctggaaat ggaagctgcg atttaagctc cagccccttc
     3001 acgcattcgt cgaaggggca tttttattgt gatctcaatc acatatccac ccgatgaata
     3061 attcttgccc aaccgctcac cgccattcct gaccgcttac gtccctaaaa taaccactca
     3121 gttatttacc ttactttacg cgcgcgtaac tctggcaaca tcactgcagg atagcggtca
     3181 atttacctcc tcaaacgcaa cgcaaaccta gaacggcttc ggcctatgat taacaaatta
     3241 ctctatgaat tttggtttgc aggaaggcag caagtgagtg aatccccggg agcttacaac
     3301 agtaagtgac aggggtgaac gaacgcaact gccgcacctg taagccaaaa gacgacgagt
     3361 aaaatgccag gttttactat ggatacgaaa aaactattca agcacatacc ctgggtgatt
     3421 ctcggaatca tcggtgcatt ctgcctcgcg gtagttgcat tacgtcgggg ggagcacgtc
     3481 agcgccctgt ggatcgtggt cgcctctgtg tcggtgtatc tggtggcgta tcgctactac
     3541 agtctgtaca tcgcccagaa ggtgatgaaa ctcgacccca cgcgcgcgac gcctgcggtt
     3601 attaacaacg acggtctgaa ctacgttccg accaaccgtt acgtgttgtt tggtcaccac
     3661 ttcgccgcta tcgccggtgc tggtccgctg gtcggtccgg ttctcgccgc acagatgggc
     3721 cacctgcctg gcacgctgtg gctgctggcg ggggtagtac tggccggtgc ggttcaggac
     3781 tttatggtgc tgtttatctc ctctcgccgt aacggcgcat ctctcggtga gatgataaaa
     3841 gaagagatgg gaccagtacc ggggactatc gcgctgtttg gctgtttctt aatcatgatc
     3901 atcatccttg ccgtcctggc gctgattgtg gttaaagccc tggccgaaag tccgtggggt
     3961 gtcttcaccg tttgctcaac cgtaccgatt gcgctgttta tgggtatcta catgcgcttt
     4021 atccgtccgg ggcgtgtggg tgaagtctct gtcattggta tcgtgctgct ggttgcctct
     4081 atctacttcg gtggcgtgat tgctcacgat ccgtactggg gtccggcact gacctttaaa
     4141 gacaccacca ttaccttcgc gctgattggc tatgcgtttg tttccgcatt gctgccggtg
     4201 tggctgatcc tcgcaccgcg cgactatctg gcaaccttcc tgaaaatcgg cgttatcgtc
     4261 ggcctggcgc tgggtatcgt ggtgctgaac ccggaactga aaatgcctgc gatgacccag
     4321 tacattgacg gtactggccc gctgtggaaa ggtgctctgt tcccgttctt gttcattacc
     4381 atcgcctgtg gtgcggtatc tggcttccac gcactgatct cttccggtac gacgccgaaa
     4441 ctgctggcta atgaaaccga cgcgcgtttc attggctacg gcgcaatgct gatggagtcc
     4501 ttcgtggcga ttatggcgct ggttgctgcg tccatcatcg aaccgggtct ttacttcgcg
     4561 atgaacaccc cgcctgctgg ccttggcatc accatgccta acctgcatga aatgggtggc
     4621 gagaacgcgc cgatcatcat ggcgcagctg aaagacgtta ccgcacacgc ggcagcgacc
     4681 gtcagctctt ggggcttcgt gatttcgcca gagcagatcc tgcaaaccgc gaaagacatt
     4741 ggtgagcctt ctgtcctgaa ccgtgcaggt ggtgcgccaa cgctggcggt aggtatcgct
     4801 cacgtgttcc acaaagtgct gccgatggct gacatgggct tctggtatca cttcggtatt
     4861 ctgttcgaag ccctgttcat cctgaccgcg ctggatgcgg gtacccgttc tggccgtttt
     4921 atgctgcaag acctgctggg taacttcatc ccgttcctga aaaaaaccga ctctctggtt
     4981 gccggtatca tcggtactgc gggctgtgtg ggtctgtggg gctacctgct gtatcagggc
     5041 gtggtcgatc cgctgggcgg cgttaagagc ctgtggccgc tgttcggtat ctccaaccag
     5101 atgctggcag ccgtagcgct ggtactgggc accgttgtgc tgattaagat gaagcgcacc
     5161 caatacatct gggtaactgt tgttccggct gtatggctgc ttatctgcac cacctgggcg
     5221 ctgggtctga aactgttcag caccaacccg cagatggaag gcttcttcta catggcaagc
     5281 cagtacaaag agaagattgc taacggtact gacctgacgg cgcagcagat tgccaacatg
     5341 aaccacatcg ttgtgaacaa ctacaccaac gcaggcctga gtattctgtt cctgattgtg
     5401 gtgtacagca tcatcttcta cggtttcaaa acctggcttg cggtgcgtaa cagcgacaaa
     5461 cgtactgaca aagaaactcc gtacgttcca atcccggaag gcggcgtgaa gatctcttcg
     5521 caccactaac cgtgtttagc cccgcttcgg cggggctttg ttcttatcag agtgaactat
     5581 gtttggtaac ttaggacagg caaaaaaata tctcggccag gcggcgaaga tgttgattgg
     5641 cattccagac tacgacaact acgtcgagca tatgaagacc aaccatcccg acaagccgta
     5701 catgagctat gaagaattct tccgcgaacg ccagaacgcg cgctacggcg gcgatggtaa
     5761 aggcggtatg cgctgttgtt aatggagaga gcatgaaccc gattgcagtt accctactca
     5821 ccggtttttt aggcgcgggc aaaaccaccc tgctgcgcca tattcttaac gaacaacacg
     5881 gctacgagat tgccgtgatt gaaaacgaat tcggcgaagt ctccgttgat gatcaactga
     5941 ttggcgaccg cgccacgcag atcaaaacgc tgaccaacgg ctgcatctgc tgttcgcgct
     6001 ccaacgagct ggaagacgca ctactcgacc tgctggataa tctcgacaag ggcaatattc
     6061 agttcgaccg tctggtcatt gaatgcaccg gcatggccga tcccggcccg attattcaga
     6121 ccttcttctc ccatgagatt ttatgccagc gctacctgct ggacggggtg attgcgctgg
     6181 tggatgcggt acatgccgat gagcagatga accagttcac catcgcccag tcccaggttg
     6241 gctacgccga ccgtattctg ctgacgaaaa ccgacgtcgc aggcgaagca gagaaactgc
     6301 gtgaacgcct ggcgcgcatc aacgcccgcg caccggtcta caccgttacc cacggcgata
     6361 tcgacctggg ccttctgttc aacaccaacg gttttatgct ggaagaaaac gtcgtcagca
     6421 ccaaaccgcg tttccacttt atcgcggaca aacaaaacga tatttcgtcg attgtggtgg
     6481 aactggatta cccggtagat atcagcgaag tttcccgggt gatggaaaac ctgctgctgg
     6541 aatcggcgga taaactgctg cgttacaaag ggatgctgtg gattgacggc gaacctaacc
     6601 gcctgctgtt ccagggcgtc cagcgcctct acagcgccga ctgggaccgc ccgtggggcg
     6661 atgaaaaacc gcatagcacg atggtgttta tcgggattca gttgccggag gatgagattc
     6721 gggcggcgtt tgcgggactc aaaaagtaac cagccctcca acatcacaat acctgagact
     6781 ttatcaccct catattcctc cagaatatga gggcattcgg gcttaattat ctctgaacgt
     6841 gcaacacttc ctgcgactcc ttatgcgcct ccacatcctg cttcgttaac cagagcgact
     6901 tacgaccaaa attttcgtac attttcagct gatcttcata gtgctgatca actgttccat
     6961 cgggagcaat aaacccactc tgaccgggtg cgaccacatc ccaggcaagc accggacgat
     7021 cgcttgtcgt tggtgagaaa acaatcatat cgttttctgt tccacggttt tgatactccg
     7081 cctgatgacg cgtttcttcc gctgcggcct gcggtacacc aaagaaatta tttgcacgga
     7141 acgttaaggc cattgcaggt gttttccagt tactcacatt attgccatag cgtttggaaa
     7201 gagtctccca ggtatcttcc agcgcagcca acacaacctc ctgctgtggt ttcccagcaa
     7261 acagatcaac cgcctgtggg attggtgatt tgtctccctg caccgcctca tacaaaattt
     7321 ttgctccaac acttatattc agcgaaccag ttgggccgtc ctgggttgtt tcgtagccac
     7381 tggcgctgta ccacttatca aatggcatag gtacggcagc cactacggta cgcttcaaca
     7441 tactggtcag ccaaacgttc aggatggcag agcctggctg ctgccaggtt ttaccatcat
     7501 cattaagcaa attgatgcca tcccaacgtg ttaatgtatc caccaactga cggcgcggat
     7561 cgctctgtgt caaaccagat gtcgctgctt gcagagtagg taaaaaaagc ctcaggttaa
     7621 gatcctgacg actggtttgg cgaataacat cccatgcctg atcagcagtt aagcgtggct
     7681 tttgctcaag cagtcggtcg atctccgtaa cgcga
//
LOCUS       DAEEKQ010000183         7681 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.185, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000183 DAEEKQ010000000
VERSION     DAEEKQ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.185"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..454
                     /locus_tag="KLK57_003617"
     CDS             <1..454
                     /locus_tag="KLK57_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBE0893888.1"
                     /translation="GAVAIASVLITGPGGNDGLEGVSYILYKDIIGVWTVCHGHTGKD
                     IMPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGALYSFVYNVGAGNFRTS
                     TLLRKINQGYIKGACDQLCRWTYAGGKQWKGLMTRREIEREVCLWGQQ"
     gene            451..921
                     /locus_tag="KLK57_003618"
     CDS             451..921
                     /locus_tag="KLK57_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005013949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBE0893889.1"
                     /translation="MSRVTAIISALVICIIVRLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDADALDAKYTKELADAKAENDALRRKLDNGGRVLVKGKCPVPS
                     SAETSSASGMGNDATVELSPVAGRNVLGIRDGIISDQTALRMLQEYIRIQCLGG"
     gene            complement(1010..1522)
                     /locus_tag="KLK57_003619"
     CDS             complement(1010..1522)
                     /locus_tag="KLK57_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HBE0893890.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1717..2481
                     /locus_tag="KLK57_003620"
     CDS             1717..2481
                     /locus_tag="KLK57_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893891.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLGWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            2432..3835
                     /locus_tag="KLK57_003621"
     CDS             2432..3835
                     /locus_tag="KLK57_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HBE0893892.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDVLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAAIIKLSWIEAAVDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLVADRFRNTFNAINNGEQYPVD
                     ELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAKREIPSPNVADAFIMAF
                     APIDTSLDIWEQLGRQA"
     gene            3835..5301
                     /locus_tag="KLK57_003622"
                     /pseudo
     CDS             3835..5301
                     /locus_tag="KLK57_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
     gene            5192..5935
                     /locus_tag="KLK57_003623"
     CDS             5192..5935
                     /locus_tag="KLK57_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HBE0893893.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            5939..7159
                     /locus_tag="KLK57_003624"
     CDS             5939..7159
                     /locus_tag="KLK57_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HBE0893894.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            7163..7669
                     /locus_tag="KLK57_003625"
     CDS             7163..7669
                     /locus_tag="KLK57_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893895.1"
                     /translation="MNNVFLYRMPVGIAGAVSRPQDLTVEPVVLKSDNAFAAYGLAGK
                     YDDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGTGKNFPGDAMKRGYVTVN
                     LGSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMSTSVSGKNVVLTNAEFTGAGDADG
                     NAEISWKI"
BASE COUNT         2048 a         1834 c         2090 g         1709 t
ORIGIN      
        1 cggggctgtt gctatagcat ctgtgttaat cactggccca ggtggtaacg atggtctgga
       61 aggtgtcagc tacatactat acaaagatat cattggtgta tggactgtat gtcacggaca
      121 caccggaaaa gacatcatgc ctggtaaaac gtataccgaa gcagaatgca aagctctcct
      181 gaataaagac cttgccactg tcgccagaca aattaacccg tacatcaaag tcgatatacc
      241 ggaaacaacg cgcggcgctc tttactcgtt cgtttacaac gtgggcgctg gcaatttcag
      301 aacatcgacg cttcttcgca aaataaacca gggctatatc aaaggcgcat gtgaccagct
      361 atgtcgctgg acatatgctg gcggtaagca atggaaaggg ctgatgaccc gtcgtgagat
      421 tgagcgtgaa gtctgtttgt gggggcagca atgagcagag tcaccgcgat tatctccgct
      481 ctggttatct gcatcatcgt ccgcctgtca tgggctgtta atcattaccg tgataacgcc
      541 attacctaca aagagcagcg cgacaaaaac gcaagagaac tgaagctggc gaacgcaacc
      601 attactgaca tgcagcagcg ccagcgtgat gctgatgcac tcgatgctaa atacacgaag
      661 gagttagctg atgcgaaagc tgaaaatgat gctcttcggc gcaagcttga taatggtggt
      721 cgggtgctcg tcaaaggaaa atgccctgtg ccatcctcag ccgaaacctc cagcgcctcc
      781 ggcatgggca atgatgccac cgtcgaactc tctccagttg ctggacgaaa cgttctcggt
      841 atccgggacg gaatcatcag cgaccagaca gcattgagaa tgcttcagga gtacatcagg
      901 atccaatgcc ttggggggta gcggtaattt tactcattat ccttcaaatc agattctgtt
      961 gtcagaggaa tgggggaagc tggattcgca agagcttttt atgggcagtt tagctaaaaa
     1021 aaggcaacac gaagctaaaa ctaccaacat aaagtgctga ccagctagca gctgaaatca
     1081 gactcactgt atacactttc actggatgaa gttgcaacat tcctgccacc aaaggagcaa
     1141 tataacgcag cacggcgata aagcgcgaag tgaaaagtat tgacacagag ttattttgca
     1201 actgaaggcg aacccgttct attgtattgg aacggcttga tattatacat gcaagttgag
     1261 ggatatgccc aattattata ccaagatggt aattaacgat agtaccgcac catgcgccac
     1321 tcatgaccgt gattccagct tcccatggtg aaagagtcgt cttgctgact gtaattacag
     1381 tggccatcat aattgatgct ggtggcaata tcgccgaaat gagtaaagtg gattttgtga
     1441 acgctattat aaaaagaagc ccccataggc gatatggatg aagtacaaaa tagttcatca
     1501 aagcatttat ccactccatc aatgtctttc cttctgtgaa tttttctttg ctatttaacc
     1561 gtgatacgac ttaatttagg cttaaacacc ttacatttac aaggtactcc tgtggggggc
     1621 cctgccacgg agcgtcgcag gagtgagtga tgaggaagaa ttaaagcaac ctattgtgac
     1681 aggcagcgat aacagtaaat cacaggaagc ataatcatgg caaaaccgga ctgggaggcc
     1741 atcgaatcgg cataccgggc cggagtcctt agcctccgtg atataggcga gaaatacggc
     1801 gttacagaag gggctatcag gaagagggcc aaaaagcttg gttgggcacg cagtggcggt
     1861 acgcaggttt gcaaaaatgg tacgcaaaaa aggaaagtgc gtaccagcag aaagcctgcc
     1921 attactggcc ttacacaaaa aagtacgcaa ctaaaaacag aatctacacc ggatacgaaa
     1981 ccgatacgcg gaatgcgtac cgatcccccg actaacccat tccaacccgg taaccagcag
     2041 gcattaaaac acggtggtta tgcccgtcgc cttctgctca aagatgaggt gatagaggac
     2101 gctaaagcgt tgacgctcga ggacgaatta tttcgccttc gtgctaacaa ccttgtcgct
     2161 gcagagaata ttggtcggtg gttggtgtcg ctggaagatg ctaatgggga ccaggaaagg
     2221 aagatgctga ttgaaaatat cagcgccgcc gagaaagcaa tgatgcgcaa tacagttcgt
     2281 attgagtcca tcgttggcac gcttgcgacg gtaggaaaaa tatttgctga tacagcctac
     2341 cgcaaggccg ccactgataa ggtgtctctg gaggctgatc gtcttcgccg tgatgcaggt
     2401 attgatgatg gcaatggaga gcgtgacctc aatgacttct actctgacat ccaaaccgac
     2461 gctgaatccg gctttacgta gtttctggac tatgcgggca cgtaacaaag tgctttatgg
     2521 tggtcggtca tcgtcaaaat catgggatgc cgctggcatt gccatatttc tgtcgaataa
     2581 atacaccctg cgtttttgtt gtgcccgtca gatccagaat aaaatcgaag agtcggtgta
     2641 taccctgctc aaaattcaga tagacaggtt tggtctgcgg caccgtttcc gtattctgaa
     2701 caacaaaatc attaaccggg ttactggctc ggaatttgtt ttttatggat tatggcgcaa
     2761 catcgaagaa attaagtcac tggaggggat cgatgtgttg tggctggaag aagcccacgc
     2821 actgacggaa taccagtgga aaattctgga gccaacgatc cgtaaagagg gttcggaatg
     2881 ctggttcata ttcaaccccg gacttgttac tgatttcgtc tggcgcaact tcgttgttga
     2941 tccgcccgaa ggcactctca tccgcaaaat taactatgac gaaaatccgt ttctgtctga
     3001 caccatgctt aaggttatcg acgcggcgcg acgccgtgat ccggatggtt ttaaacatgt
     3061 gtatgagggc gttccggagt ctgatgatga tgcggcaatc atcaaactgt cttggataga
     3121 agccgcagtg gatgcgcaca aaacgttaaa tttcgaaccc agtggaagaa agcgtattgg
     3181 ctttgacgtg gctgacagtg gtacagataa gtgcgctaac gtttaccgtc acggatccgt
     3241 tgttttctgg gccgacgaat ggaaggccaa agaagatgaa ttactgaaga gctgccagcg
     3301 tacttatcag gcggcgctgg agcgtgaagc agatattgtt tacgactcta tcggtgttgg
     3361 tgcgtctgcc ggtgctaaat tctctgaaat taacgctgac cggaagagcg agaacgcata
     3421 cgcgcgacgt gtgaattacc agaggtttaa cgccggtgct ggtgtgcatg agccagatga
     3481 cgagtacaac ggcatcccca acaaagactt tttcgcaaat cttaaggctc aggcatggtg
     3541 gctggtggct gaccgtttca gaaatacgtt taacgccatt aacaacggag aacagtatcc
     3601 tgtggatgag ctgatcagca tagattctcg ttgtccgttg cttgaaaagc tgaaactgga
     3661 actgacaaca cctcatcgtg atttcgaccg taacggacgt gtgatggtcg aaagtaaaaa
     3721 agacctcgca aaacgcgaga taccgtcacc aaacgttgct gatgcattca ttatggcctt
     3781 cgcgccaatc gatacatcgc tggatatctg ggaacagctg gggagacagg cctgatggca
     3841 cgaaacaaac aagccctgcg gcgaactgtg caggccacag ctgatggtta tgagaatttt
     3901 attgcccgcg tagggatgca gacacctaac cagcactcag catccaccta ccgggctaat
     3961 ttcaccagtc gtaaccgcat gctggtggaa tggtcctatc gttcatccta gatcatcggc
     4021 gaagcagtcg atgctatccc ggatgatatg acccgcaaag gcattcgcat cacttcggaa
     4081 attgatgcaa aagatcgtgg cattctcgaa tcacaactgg atgagttgca aatctgggat
     4141 gcgctgaatg acgtgctgaa atggtcgcgc ctctacggcg gcgcggtggg tttcatcatg
     4201 attgaggggt aggcaccaat gaccccgctg cgacccgaaa ccatcggtaa gggcaagttt
     4261 aaggggattc tcccgctcga ccgctggatg gtcgacccgg cactgacccg ccgcattaaa
     4321 gatatggggc cggacctggg taaacctgag ttttacgatg tggtgaccac agcaacggga
     4381 attcctgcct ggcgcattca tcacagtcgc ctgattcgct ttgatggcgt cacgttgcca
     4441 tttcagcaga agatgaccga gaacgaatgg ggaatgtcgg ttgtagagcg tatctgggat
     4501 cgtcttaccg cgttcgacag cgctactgtc ggcgcggcgc agctggtcta caaggcgcat
     4561 ttgcgtacct acagcgtgga gaagctacgc gagcttatcg cacttggtgg tcctgcgtat
     4621 gaagcgttgc tgaagaatat cgacctgatt cgacagttcc agagtaatga aggcatgacg
     4681 ctcatggact cgcgggataa gtttgaaacc catcagtaca gcttcagtgg tctggatgac
     4741 atcctttcgc agtttgtaga acagattagt ggcgctgttg gtatcccact ggtgcggttg
     4801 ttcggacagt ccccgaaagg attttctacc ggtgatgcag accttgccaa ctattacgat
     4861 cgcatcagtt cgttgcagga gaggcgttta cgtcttccgg tgcggcggat actggacatc
     4921 atgcatcgtt cggaacttgg caagccgctg ccggacgatt tcacgtttga gtttaacccg
     4981 ctctggcaaa tgtctgatgt cgatcgctca acggtggcgt taaacaccac caacgcaatc
     5041 agtacggcgc tgggtgatgg tctgatgaca ctgaaagccg ctatgactga tttgcgagaa
     5101 aattctgacg taaccggcat cggggcatcc attaccgacg aggacatcga gaatgccgaa
     5161 gatgaagcgc cgcccggcat cggcgaacct gatgacgaac cgcaggagcc gtcaggcgga
     5221 aatccggtat cgaaccagcc tacgcaggat agcgagggcg gtcggagaca tcgtaaatgg
     5281 tcgctacgat ggttcaaatg acagtatcac ggaaattatt gcggcgctgg agcgttacag
     5341 tgaaatcatc accccctggg cgacaaaggt cgcggaaaac tttactgcgg acctaacccg
     5401 gcagaacgag aaagtttggc ggcaacacag caagaacatc agtcgcgagc tccgcaatct
     5461 tgtggaaagc gctcctgtgg gccaggtgat gcaatccatc atcgccgaac aggtcaagta
     5521 catcaaatct ctgcctctcg aggccgcaga cagggtgtac gacatccaga acaaagcgat
     5581 agaggctgtt gtcaccggtg gtcgggcgga gcagtttgct aaagagattg catctaccgg
     5641 cgatgttgct aagtctaggg ccgatctgat tgcccgaacg gaactgggaa gagcaacggg
     5701 cgcgctggat atggcccgag cgatagctat tggctcggat ggttatattt ggcgtacagc
     5761 cgatgatggc gatgtccgag attcccacga tcacatgaaa ggtaaattcg tccgctggga
     5821 ttcacctcca actttggacg gcatgaccgg ccacgcgggc gagctgccaa actgccgctg
     5881 ctataaagag atcgtgtttg ttcgcgttcc attcgcaatg aaaagggcag cataacccat
     5941 gaaatacttt tttgagacca ggctcgggga aacccgatac cgcctggctg acggctcgtt
     6001 gctgtgcaaa gacgtgccga taggacgaac aggtaagcag ctctatggtg ctgatgacct
     6061 gccaaaactg aaacccgata agttcggtga aatagtcgtc acgcgttctc ctgagcaggt
     6121 attccatccg gccacgcttg cctcattcga agggatgagc atcacgatcc tgcatcctga
     6181 agatgaaaac gggaatgtgc ggctggtaaa tcccgagaac tggaaagagc ttgctgtcgg
     6241 gcacctccag aatgtccggc gcgggacggg tgagcagtct gatttgatgc tggctgacct
     6301 tatcgtcaaa gacgaaagcg ccattcagct tatcgaagat ggcctgcgcg aagtgtcgtg
     6361 cggctatgac gcggagtacg agcagaccga gccaggtaaa gccgagcagg tcgatattac
     6421 cggaaaccat gtggctcttg tccctaaagg cagagccgga aatcgttgtg caattggaga
     6481 cagagacaca atggcaaatc aaaagaaaaa ctggtggaac cgcatgcgtg cggccatcaa
     6541 gacaggagat gccgacacca tgaacgaact ggtggagtcg gctcccgcat cggttacagg
     6601 agatgagggg gatttgccgc agggcgttaa tctcaacatc aacctgtccc cgcagcaacc
     6661 actaccggac aaagcaccag agatgggcgg agatccaacc ggcgacagtg atgatgacct
     6721 caaaacatta ctgaaagccc tgctggctaa gctggaaaga aatgccacgg gcgataacga
     6781 taataagcct gacgataatc cgaccggtga cggcgaggac gatgaagagg aaaccacgat
     6841 tactggtgac tcagcctggc gtgccgaagt tatcgttccg ggtatcgatc tgagccgtaa
     6901 gatgaaaccg accgcgttca aacgcgaggt tctggcttct gctgacaaaa cgctggttcg
     6961 ccagatagtc ggtgatgcgg atatccgcaa attaccgaaa caatcggtcg acatggcgtt
     7021 taatgccgtg tctgaggttg ccaaagggcg aaacacccgc gccaccaccg gcgatgcaca
     7081 gcgcctaaac atgggcatga ccagtatcgc ttccctgaac aaacaaaacg ctgaattctg
     7141 ggcaaaccgt aaagggtaaa aaatgaataa tgtatttctg taccggatgc ctgttggcat
     7201 tgccggggct gtctctcgcc cgcaggactt aaccgtcgaa ccggtggtcc ttaaatccga
     7261 taacgccttc gctgcctatg gcctggctgg taaatacgat gatgacggtt ttttcgtgcc
     7321 gctggcagat ggtgataccg cagacaaggt gaaggggatc tacgtgcgcc cttatccgac
     7381 cacgtcgcag ccggacatgg ttcgccaggt gggaacaggc aagaacttcc cgggcgacgc
     7441 catgaagcgt ggctacgtga ccgttaatct cggttctgat tttgatgcca gcactatcaa
     7501 aaaaggcgat ccggtatacg ttgtcgtctc cactgatgaa tccatcaaag tgccgctggg
     7561 tggattcatg tccacgtcag tcagtggcaa aaacgtggtg ctgaccaacg ctgaattcac
     7621 aggtgccggt gatgctgacg gcaatgcaga aatttcctgg aagatttaag gaacagacga
     7681 a
//
LOCUS       DAEEKQ010000184         7524 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.186, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000184 DAEEKQ010000000
VERSION     DAEEKQ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7524
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.186"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            229..1182
                     /locus_tag="KLK57_003626"
     CDS             229..1182
                     /locus_tag="KLK57_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBE0893896.1"
                     /translation="MEKFHMFDDRIYQMTNGVKAADRIIYTVMLNEQKFYHDKGQYYT
                     PSYAHLGQYAGITRTSVIACMKQLEAHGLIKKTNVASNQCVIKVYSLDEVPEVLTELT
                     AKEARKAKLESKVTQSVKKDDPLSQNSLPTESKFFTQSVKIFDTYNNSNNKKELINNN
                     KEDDFEEEYFKKGASLCDSHECDSLGIEEAIKETVKTSDLDDLDSEAAKEVVKKEDKI
                     SDVIASSDHKNTQCPATDDELPDVDLDDEEDPEKELKREQRKAEIKERFRNAKLKNKV
                     SDLDDFDDSDDDFTFTPKKKTHTEPKKEKVKVRRVYGGGMF"
     gene            1182..1478
                     /locus_tag="KLK57_003627"
     CDS             1182..1478
                     /locus_tag="KLK57_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893897.1"
                     /translation="MKTYNGERKYRDYLLRRYSISREGYLMKDTHGEVYRIRPKKEGR
                     DYYFVDDVTDLKIDALKFAVLYHYDLWDSVHQLRLKDGDPGNLRATNIIKGKCR"
     gene            1802..2092
                     /locus_tag="KLK57_003628"
     CDS             1802..2092
                     /locus_tag="KLK57_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893898.1"
                     /translation="MTHTVTRLLPEAFERLAAIKAKYPDMKHTALVGEILKESVNLSP
                     FDNVKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADRLEV"
     gene            2313..2528
                     /locus_tag="KLK57_003629"
     CDS             2313..2528
                     /locus_tag="KLK57_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893899.1"
                     /translation="MKTITVQLQTNKAFRYFENLLELYEGWGSIHGKDDIYLHLSAPN
                     YSLKTPVKQSWLKDYGHQMGLLVSDLS"
     gene            2675..2980
                     /locus_tag="KLK57_003630"
     CDS             2675..2980
                     /locus_tag="KLK57_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893900.1"
                     /translation="MNYPMQIMVDQETMNWLIDLNRSGFNIYFMHCFEASTYIDKHII
                     NEIKLGDYVHKLEVTDPYNKRFIIEECNRLDLSPEQLLKLNVFLTYQNELNPYSEVM"
     gene            3456..4166
                     /locus_tag="KLK57_003631"
     CDS             3456..4166
                     /locus_tag="KLK57_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001681134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0893901.1"
                     /translation="MTNDIYINGKVIDSFSDWSPQTEHPAITIQRKEHDKRIELEQSL
                     KMSPKTTIFVSPEPQEMPDICKSEALLELEKKYYQLLKEQRLRLDDAYSKVTLMQSAI
                     EPSEFEIQDELSQKPFVYYQYEDNDGFGTFQENVAAVISSLPAGHRIVKMVKASRGAG
                     QFIYMTNKSDEELAEIARQNILASRNKQLDNVKLYLSRELQAMKELVKEYEDAKKIAM
                     QADIEQLTKISSKYAKAI"
     gene            4191..4397
                     /locus_tag="KLK57_003632"
     CDS             4191..4397
                     /locus_tag="KLK57_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893902.1"
                     /translation="MIDLTERYELIKAISERLKEQPNKPKRIKSFTSSKQKNRIRKIA
                     KWCLDKLPSDCQIVGEDGNYYLVR"
     gene            4496..6676
                     /locus_tag="KLK57_003633"
     CDS             4496..6676
                     /locus_tag="KLK57_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBE0893903.1"
                     /translation="MSKFTVDSFIVELSFSENVIKGLQRVEKAALQSAQRIERNLNRA
                     FKVDTKQLDSNLTSSLGQLERKFNKTFDKIEQRARSTKAFQFATRFNDTVNPPKQSRQ
                     PRISGNRAITAAHSVNMSKLGDINPLMAKMFKAQYYSLSGKAGNIGSEKFNAELAKLN
                     QSLRETLNKLRSQTKTASINNTSDAFNNLASNAIKLSGAFYSVMGALNAYKAIMNAGL
                     KRDSAQRAAKFVLGDKASEAEVFIRNLADKTGLNISEGLSSYAKFAAGAQGSMSQEQT
                     QELFGNATAMSRLMGLSNDELNGILRAFEQMASKGKIQAEELRGQLGDRMAGAFKLFA
                     EALGMTATELDAAMKNGKVLSADTLPKVAKQMGLMIDKAGGWAEVTKSTQTALGKLAN
                     NWDDTMVKIFSGSQDELNGFLSSLSNLLSEMGMSSSIAGDAIGGLIDMLKAGVDDIRV
                     FNNTIEGWILQTKKFYYSLDDTKRKLLDEVGDGFINFVKGLAIALSAKTLFSATTGVM
                     NLTRAITTLGTRANQVAGQVATGKGGGKLKGVAGGVGSALAIGYADDGYETALALASM
                     IPQIRGVTLGLYALKKALDFMNQEVVKNANMHPSGVGVGSDFNPVYSGDPNKPNMINE
                     GWSRIFSSMGELFNNATMNIARFNHPELNNIKQDSALTSADIQGLRDEISALSKRIQE
                     PVKVSLGGQVVVRPDEPSFFTFNQSANDQFTQDMLLSSSYPEEE"
     gene            6833..7421
                     /locus_tag="KLK57_003634"
                     /pseudo
     CDS             6833..7421
                     /locus_tag="KLK57_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         2481 a         1336 c         1618 g         2089 t
ORIGIN      
        1 aactcagaac attaagaggt tacaacgttg ggaggcgtca gggtattgac agattttcac
       61 ggcctcttat tcaatcaagg cttttccttt tgctgttaat actcgcgtaa tcgctcaggc
      121 ttctgagttc ctttatcagt ccatcttcta attaattcac tccctctggg gttggtcact
      181 atacgtggtc agccctttct ttgttttatt tttaaaaagg aaaatataat ggaaaaattc
      241 cacatgtttg atgaccgtat ttatcagatg actaatggcg ttaaagctgc cgaccgtatt
      301 atttacactg tcatgttgaa tgaacaaaag ttttatcacg ataaggggca atattatacc
      361 ccttcatatg ctcatcttgg tcagtatgct ggaatcacaa gaacatccgt gattgcatgt
      421 atgaaacaac ttgaggcaca tggtttaatc aagaaaacta acgttgcaag caatcagtgt
      481 gttattaaag tttattcact tgatgaagta ccggaggttt taacagagct tacagctaag
      541 gaagccagaa aggcaaagct ggagtctaaa gttactcagt cagtcaagaa agatgaccca
      601 ctgagtcaaa attctttacc cactgagtca aaatttttta ctcagtcagt caaaatcttt
      661 gacacgtata ataacagtaa taacaagaaa gaacttatta ataacaataa agaagatgac
      721 tttgaagaag aatattttaa gaagggagct tcgctctgtg attcacatga atgtgattct
      781 cttggtattg aagaggctat taaagagact gttaaaacat ctgacttaga tgaccttgat
      841 agtgaagctg ctaaagaggt cgttaaaaag gaagataaaa tctctgatgt gattgctagt
      901 agtgaccata aaaacacaca gtgccccgcc actgatgatg aattaccgga tgttgacctt
      961 gatgatgaag aagaccctga aaaggaattg aaacgtgaac agcgtaaagc agaaattaaa
     1021 gaaagattca gaaatgcgaa gctaaagaac aaggttagtg acctcgatga ctttgatgat
     1081 agtgacgatg attttacttt tacaccaaaa aagaaaactc acaccgagcc aaagaaagaa
     1141 aaagttaaag tcagacgtgt ttatggtgga ggaatgtttt aatgaaaact tataatggcg
     1201 agagaaaata ccgtgactat ttactcagac gttacagcat ttctcgtgaa ggttacttga
     1261 tgaaagatac tcacggggaa gtttaccgga ttcgaccgaa gaaagaaggg agggattatt
     1321 atttcgtcga tgatgtgaca gatttaaaga ttgacgcttt gaagtttgcg gtgctgtatc
     1381 attacgattt atgggattca gtccaccagc tacgattaaa agacggtgac cccggtaatc
     1441 tgagggccac caatattatc aagggaaagt gccggtaagg ccaccagcta tttatttact
     1501 gaaatctcta tctcaacacg aagaagttta agtcgaattg taagctttgt cttttttgta
     1561 atgactcgat tcataacgca aaacaccaat agcacaataa ataccgcaaa gcccaacgag
     1621 ctaaaacgat gagattcaat aattgctgtg aaaaaatcat gcattaattt tccttttgtg
     1681 aaaggaaaca gcacctgtat cagcccctac aaccatagta tcatgtaaat gaggtactga
     1741 caatatgtta aatagatgtt aagattgtag tatgagtaag agatttaagg agatttttac
     1801 aatgactcac acagttacgc gccttttgcc agaggcattt gaacgactgg cagcaatcaa
     1861 agccaaatat cccgatatga agcacacagc gcttgttggg gagattttaa aagagtcggt
     1921 aaaccttagc cctttcgata acgttaaggg cgtcgcagag ggttctaagg ccatcagaat
     1981 cacaacgggc caactggaac tactcaaagc acagtccgca gtagcaggtt gctcaatgat
     2041 tcaggtgtta agcgctaagg tccatcaact ggcggataga ttggaggttt aatgtgttat
     2101 aggttccaat attccacact tggccgccac ctaatcagac agaatttagc agatgctgaa
     2161 tttgaagatg tgaccctcaa agcaaacaaa cgattaaaac cctctcaaat ccaccagctt
     2221 aaacacgaat taaagccact ggtaatactc gactataacg accctgaaca gcttgagcag
     2281 ggaattaagc ttatttctga atggggtgct tgatgaaaac tatcacagta caacttcaga
     2341 ctaataaagc atttcgttat tttgaaaact tgcttgagtt atacgaagga tggggaagta
     2401 tccacggcaa agatgatatc tatcttcact tgtcagcccc taactattct ctgaagactc
     2461 cggttaagca aagctggtta aaagactacg gtcaccagat ggggctatta gtctcagact
     2521 tgagctaaaa acagcaaaac atatctctca ggctttacca gacgcaccag aaacgttttt
     2581 aagcccctaa tcagggtaac ttagggaatc gccttaagtg gtctaaaaaa ggggttaaaa
     2641 cgtcttagaa tgcgatacag gaaggatttt aaaaatgaat tacccaatgc aaattatggt
     2701 tgaccaagaa accatgaatt ggttgattga cctgaacagg tcgggcttta atatctattt
     2761 catgcattgt tttgaagcgt caacgtatat tgacaagcat ataatcaacg aaattaaact
     2821 tggtgactat gttcacaagc tggaagtaac agacccatac aataagcgct ttatcattga
     2881 agagtgcaat cgacttgatt taagccctga gcaattattg aagctgaatg tattcttgac
     2941 ctatcagaat gaattaaacc cttacagcga ggtgatgtaa tgacactcgt tattctctgg
     3001 atgactttag ggttatttgt tggttgctgg gtaggtaata cggtaaataa gaattgatgt
     3061 taccaatctg ttaaaaagct gttgacaaat aaccttaagc atgagatgat ggtaataggg
     3121 aagagcaact cttcctgaat taaactatta atcattctac atcaatgata gttattctga
     3181 aaggtgcaat gcttttcgct acatgacctt tcaggttagt ctgagaggtc atctcgaaaa
     3241 gtactcttgt tcattcgttt gaatcctttt tgaatccttt agatgtttta atttcttcac
     3301 cttatgaatg gccctttata agaaattatc tagggctatt cagcttttaa aaagcatact
     3361 tggcaaaccg ccttttaagt ctcttctaag tttagatagg ctgtcaggtg tgctttttaa
     3421 aaactgaaac acttccgctt agaagaggaa aagcaatgac aaatgatatt tacattaatg
     3481 gcaaagtgat tgatagcttc agtgactggt cgccacagac tgaacatcca gctatcacta
     3541 ttcagcgtaa agagcatgac aagcgtattg agcttgagca atcgctaaag atgtcaccaa
     3601 aaacaaccat ctttgtgtct cctgagcctc aagagatgcc tgatatttgc aagagtgaag
     3661 cattacttga gcttgaaaag aaatattacc aactgctgaa agaacagcgc cttagattag
     3721 atgacgctta cagcaaagtt accttgatgc aatcagctat tgagccgtca gaatttgaga
     3781 ttcaggatga attgtcacag aaaccatttg tctattacca atacgaagat aatgatggtt
     3841 ttggtacttt tcaagaaaac gtcgcagccg tgattagtag tcttcctgct ggacatcgaa
     3901 ttgtgaagat ggttaaggca tcaagaggtg ctggtcagtt tatttacatg actaataaga
     3961 gcgatgaaga actcgctgaa attgctagac agaacatctt ggcatcacgt aacaagcaac
     4021 ttgataatgt taagttgtat ctctcaagag agcttcaggc tatgaaagag cttgtgaaag
     4081 aatatgaaga tgctaagaag attgctatgc aggctgatat tgagcagctt acgaagattt
     4141 ctagcaaata tgcaaaggct atttaattaa taattaaaag gtggtggtga atgattgatt
     4201 taacagaacg ctatgagctt attaaagcaa ttagtgaacg attgaaagaa caacccaaca
     4261 aaccaaaacg aatcaaatca ttcacctcct ctaagcaaaa gaatagaatc aggaaaatag
     4321 ctaagtggtg tcttgataaa ttaccaagtg attgtcagat tgttggtgaa gatggtaatt
     4381 attatctggt taggtaacaa gattggttgg gtgaaaatct acgggagaga ttatcgagtg
     4441 atatcggtag tctttccctt tttcgtatat gaacacttaa agaggtgaaa catgaatgag
     4501 caaattcaca gttgatagtt ttatcgttga acttagtttc agtgaaaacg ttattaaggg
     4561 cttgcaaaga gttgaaaagg cagctttgca gtcagcacaa agaattgaac gcaatttgaa
     4621 tagagcgttt aaggtcgata ccaagcaact tgacagcaat ctgacaagtt ctttagggca
     4681 gcttgaaagg aagtttaata agacctttga caagattgag cagagagcaa gaagcacaaa
     4741 agcatttcag tttgccacca gatttaatga cactgttaat cctccaaaac aatcaaggca
     4801 accacgaatt agcggaaaca gagcaatcac agcagcgcat agcgtgaata tgtctaagct
     4861 tggtgatatt aatccgctga tggctaaaat gtttaaagca cagtattaca gcttaagcgg
     4921 taaggctggt aatattggaa gtgaaaagtt taacgctgaa cttgcaaaat taaatcaatc
     4981 tcttcgtgaa acgttgaata aactcaggtc acagacaaag accgccagca ttaacaatac
     5041 aagcgacgca tttaataatc ttgcaagcaa tgcgattaaa ttatctggtg cattttactc
     5101 tgtgatgggt gctcttaacg cttacaaggc cattatgaat gcaggtctta aacgagattc
     5161 cgcacagaga gccgctaagt ttgtacttgg tgataaagca tctgaagctg aggtatttat
     5221 tcgaaattta gcagacaaga caggtcttaa tatctctgaa gggctatcaa gctacgctaa
     5281 gtttgcagca ggtgctcagg gttctatgtc acaggaacag acacaggagc tatttggtaa
     5341 tgcaacggct atgagtcgct taatggggct ttctaacgat gagctaaacg gcattttgag
     5401 agcatttgag cagatggcga gcaagggcaa gattcaggct gaggaattac gtgggcagtt
     5461 aggtgaccgt atggctggtg catttaagct attcgctgaa gcattgggta tgactgctac
     5521 agagcttgat gcagccatga aaaacgggaa ggtactttcc gctgatactc tccctaaagt
     5581 cgctaaacag atgggcttga tgattgataa agcgggtggc tgggctgaag tcactaagag
     5641 cactcagaca gctttaggta agttagcaaa caattgggat gatacgatgg tgaaaatctt
     5701 ctcaggttca caggatgagc taaacggctt cttatccagc ctgagtaacc tgcttagtga
     5761 aatggggatg agttcttcta tcgctggtga tgctatcggt ggacttatcg acatgcttaa
     5821 agctggtgta gatgatattc gcgtattcaa taacaccatt gaaggctgga ttttacagac
     5881 taagaagttc tattacagcc ttgatgacac taaacgaaaa ctacttgatg aagtcggtga
     5941 tggttttatc aacttcgtca aaggtcttgc tatagcgtta tctgcaaaga cgctattcag
     6001 tgctactaca ggggttatga acctgacaag agcaatcaca acgttaggta caagagctaa
     6061 tcaggtggct ggtcaggtag ctactggtaa aggtggtgga aaactgaaag gtgtagctgg
     6121 tggtgtaggt agtgctcttg caattggtta tgctgatgac ggttatgaaa cagcgttagc
     6181 attagcttca atgattccac aaatcagagg cgtcactctt ggtttatatg cattgaagaa
     6241 agcactggat tttatgaatc aggaagtagt caaaaacgct aatatgcatc cgtctggggt
     6301 aggtgtgggt agtgacttta atcccgtgta ttctggtgac cctaataaac cgaatatgat
     6361 taacgaagga tggagtagaa tttttagttc aatgggtgaa ttgtttaaca atgccacaat
     6421 gaatattgca agattcaatc atccagagtt aaacaacatt aagcaagatt cagcattaac
     6481 gagtgcagat attcagggtc tgagggatga aattagtgct ttgagtaagc gtattcagga
     6541 gccagtgaaa gtttcacttg gtggtcaagt tgtcgttcga ccagatgaac catctttctt
     6601 tactttcaat caatcagcta atgaccagtt cacgcaggat atgctcttaa gttcaagcta
     6661 tccagaggaa gaatgattta acggtgttct acgtgaataa agcaaagtta atacccacac
     6721 caaaaattat ttttcacgta gctcacgctt aacagagaaa atacatatat atcaaaggct
     6781 tggttaaaaa tgattaaaat acactaagcc tttgaaatca aaggagaatt taatgattta
     6841 cactaatcag cttcgttaca gtggcaaaga tggcatctat ttccacttaa gagacaatgt
     6901 agatgccttc ttaaccttat cagcaactga aaacatggaa tttgatagcc ctatgcaggt
     6961 gactacacag aacatgcaat tatgaaggaa gcttattatt aactcgtcag ggtcaattag
     7021 tagaaaactt cattgacact ctggaaaact ggcgtgacca gaagcagatt atttctgtta
     7081 tctgtaaaga tggaattaaa attgacgatt ccattattac caactttaaa gcctctaaag
     7141 atgttactat ctctaacggt cttcgtatcc agctaacttt tcaggaaatt aactttaaag
     7201 caatcgtagg tcaaactgat atttcagcag ccactggcaa aacggctacc acaaatgacg
     7261 gtggtgtgac cagtaagaag aatgcaggta acgtgaccac tgaacgcaat aacgcgccta
     7321 tgctatcgtg caaagagtta atgaatatta gcgatgtatc cggttacgct ccagctatca
     7381 gaaatgatat tctcgatgct cagattaaat gtaataaggc ttggaagctg gactattcag
     7441 acttaacaga agctgaaaaa accaacgtca ccaatgctaa tagtaacgct ggtcgagtag
     7501 cttcaaagct taaaaagtac tcag
//
LOCUS       DAEEKQ010000185         7489 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.187, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000185 DAEEKQ010000000
VERSION     DAEEKQ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.187"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(149..1231)
                     /locus_tag="KLK57_003635"
     CDS             complement(149..1231)
                     /locus_tag="KLK57_003635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001053102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HBE0893904.1"
                     /translation="MNLPVVPPLNVIRERLQLIFPEGTENRGYLIREIAAKTIFVMFY
                     AGAVEGLGRWIRPSQVVSMGDSQAALTDDASRESWVKMTFSKQKIRPADAWYAENTRE
                     PIRDETIKNGLIPCNAIVERKGIPTTSSRPRYSLNRSFAALFDAGLEGDSLLSAISSW
                     QENHLNKAALARLRLLKSGAVIAADAVVVTFPNGEKRTLAPGPSSTIAKAVIEVFAPN
                     FLKNPTVLWLSESGNKVVAQDDVLAKALGLNIDASKALPDIILIDLGEDRTGSDMLVV
                     FTEVVATDGPINRERKVTLTHLAIDAGFSEKNLAFLTAFMDRSALPFKKAIPELAWGS
                     YAWFASEPEHLIDLRDGYPVKISQRK"
     gene            complement(1228..3024)
                     /locus_tag="KLK57_003636"
     CDS             complement(1228..3024)
                     /locus_tag="KLK57_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020803211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HBE0893905.1"
                     /translation="MNVQHFDFFTDNEFSNLERYQTCKAMAKGYAGNYKTGQSRLMHA
                     RSYCFSIVAAYWDTLAKKQKSSIKMRAVPHTVYIEDIPCDARELAERTGELIAQFPAE
                     DAGYLIGSIYTVMLPSAYRSELGAYYTPPPLVARLLDLAEKSGVDFSHASVIDPACGG
                     GAFLAPVAIRMLKKDKGSSPEWMLRRISRRLKGIEIDPFAAWMSLVLLESVLMPLCVK
                     VKRRLPEDTIIVADALQQDKFSGYDLVVGNPPYGRVTLDIKTREKYSRSLFGHANLYG
                     LFTDLAVRMVKEKTGVIAFLTPTSFLGGQYFTALRTLLTEKTTPYALDFVTDRDGVFD
                     DVLQETMLTAFKAGEHKVRAQVSSLVPKGLNKAKIEKIGNVDIEKGGATWLLPRVKDD
                     ACFLNVLKQMSTRLADLGYSVSTGQLVWNRFKSQLRTTKGKNSYPLVWAESITSAGFR
                     FSADRKNHVPYIDITPQQGFLVTKSECVLVQRTTSKEQDRRILAAILPQGFIDETGGV
                     VVENHINIVYSNGLFSAVRPDVIDMLLNSHVVDRAFRCISGSVAVSAYELNSIPLPSL
                     EQVMEIQSLIDAGVHRRIIERTIAGFYGVMIA"
     gene            complement(3275..3631)
                     /locus_tag="KLK57_003637"
     CDS             complement(3275..3631)
                     /locus_tag="KLK57_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893906.1"
                     /translation="MEIKPEDELSNIVLFPVKEDNPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEERERRKNIEKLIQIE
                     RNAKARIRRVTKYSPE"
     gene            complement(3689..4111)
                     /locus_tag="KLK57_003638"
     CDS             complement(3689..4111)
                     /locus_tag="KLK57_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBE0893907.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEAKTGATRYLIS
                     VLARELVASGDVYNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSVVDPDLIWSLPDG
                     EIRRYDRHQNIICRECRKSEVMQRVPAFYQGDFQEAVL"
     gene            complement(4127..4852)
                     /locus_tag="KLK57_003639"
     CDS             complement(4127..4852)
                     /locus_tag="KLK57_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000450647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1627 domain-containing protein"
                     /protein_id="HBE0893908.1"
                     /translation="METVFDALKALKRASSHVVASRLGISREDAVNELWKLKRRGEAD
                     NKGSMWWLIQAGESEPVSPVPKVTAQMLTEAIEQHGPQTADELALMFGITSRRANSSL
                     AMAISKGRLIRVNQGGKFRYCIPGADLPAEPKAASIAETDGKVFPQPAGVALPVQEAA
                     TQEEIKTETVADIVQSLPSFTETQADDLVLPSLHMANRELRRAKSHVQKWERVCAALR
                     ELNKHRDIVRQIVDSSGRIVSEK"
     gene            complement(4874..5620)
                     /locus_tag="KLK57_003640"
     CDS             complement(4874..5620)
                     /locus_tag="KLK57_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE0893909.1"
                     /translation="MKNIAAAGVLERIHRLAPQGAVPPYRTVEEWREWQLAEGRKRSE
                     EINRQNRQLRVEKILNRSGIQPLHSKCSFANYQVQNDGQKYALSQAKSIADELMTGCT
                     NFVFSGKTGTGKNHLAAAMGNRLMAKGRSVIIVTESDVMSVLHDSYDNGKSGEKFLQE
                     LCGVDLLVLDEIGIQRETKNEQVVLHQIIDRRTASLCSVGMLTNLNHAAMSTLLGERI
                     MDRMTMNGCRWVTFNWDSWRSNVSFPGVVK"
     gene            complement(5627..6691)
                     /locus_tag="KLK57_003641"
     CDS             complement(5627..6691)
                     /locus_tag="KLK57_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HBE0893910.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDTDKDTDKEDQNTM
                     VHGVKNATNQAGDVQTVNPGQPAGSTPEADSAYALKADSGAVQQVMTARPEQSLQLQQ
                     PEADSAIQREADRVAPENTGQPVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWKAR
                     LREGVPPETMLDGVRRYARYLAATGKAGTEFVQRATTFFGPDRNFENPWLLPVSGTNN
                     QRCVNHISEPDTEIPPGFRG"
     gene            complement(6763..7188)
                     /locus_tag="KLK57_003642"
     CDS             complement(6763..7188)
                     /locus_tag="KLK57_003642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBE0893911.1"
                     /translation="MKIKHEHIRMAMKAWAHPDGEKVPAAEITRVYFELGMTFPVLHN
                     DSKHNTLYLNTQKIFRWLDKDTPDAVEKIQALLPAIERAMPPPLVARMRSHSSAYFRE
                     LVETHERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(7185..7448)
                     /locus_tag="KLK57_003643"
     CDS             complement(7185..7448)
                     /locus_tag="KLK57_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0893912.1"
                     /translation="MNLGEYLHHFHITQSSFAEIVGASQGMVSHVITGRAKLTGEKIL
                     RWCEATGWVVTPHEIDSKTYPNPTDGLPAEFQANTQPSAGVDS"
BASE COUNT         1830 a         2048 c         1553 g         2058 t
ORIGIN      
        1 cacagaacaa ttattgcttt tgccgttgat gacaataaag tttttatcgc aggcgtatac
       61 cacggtgggc aaatctatga aaccgatttt ttatccagtg caccgtaaaa cccccatcct
      121 tcagggcgtg gcggatatca aactttggtc attttctctg gctgatcttt accggataac
      181 catcccgaag atcaattaag tgctccggtt cagaagcaaa ccatgcataa gatccccacg
      241 ccaattctgg tattgccttt ttaaaaggca gagcacttct gtccataaat gccgtaagga
      301 atgcaagatt tttctcgctg aacccagcgt caatagccaa atgagtgaga gtaacttttc
      361 gttccctgtt tataggccca tctgtcgcaa ctacttcagt gaaaactaca agcatgtccg
      421 aacccgttcg gtcctctccc aaatcaatca aaataatatc tggaagtgct tttgaggcat
      481 caatattcag ccccaatgct tttgccaata catcatcctg agcaacaact ttattcccgg
      541 attcagacaa ccagagcaca gttggattct taagaaaatt aggagcaaaa acttcgatga
      601 cggccttagc tattgtactt gacgggcccg gagctaaagt tctcttctct ccatttggga
      661 aagtgacgac tacggcatca gcagcaatca ctgcccctga tttcaataaa cgcaacctag
      721 caagagcggc tttgttcaaa tgattttctt gccaggaact tattgcactc agcagcgaat
      781 ctccttcaag cccggcatca aagagtgctg caaaagagcg attcaaactg taacgaggac
      841 gagaggatgt ggtgggtata cctttacgct caacaattgc attgcaaggt attaggccat
      901 tttttatagt ttcatcacgt attggctcac gcgtattttc cgcataccat gcatcagcag
      961 gccttatctt ttgtttagag aaagtcatct ttacccagct ttctctggac gcatcatcag
     1021 taagagcagc ctgcgaatct cccatactta caacctggct gggccttatc catcgcccca
     1081 gcccctcaac cgcaccagca taaaacatga caaaaattgt ttttgcagct atttcccgaa
     1141 ttaaatatcc acgattctca gtcccttcag ggaaaattaa ttgtaatctt tcccggataa
     1201 cattcaatgg gggaacaaca ggcagattca tgcaatcatc actccataga aaccagcgat
     1261 agttctctca ataatccgtc tgtggacgcc agcatcaatt aacgattgaa tctccatcac
     1321 ctgctccaga gaaggcaacg ggattgaatt caattcatag gccgaaacag caacacttcc
     1381 gcttatacac ctgaacgccc gatcgacaac atgcgaatta agtagcatat caataacatc
     1441 agggcgaacc gcagagaaca atccgtttga atatacaata ttaatatggt tttcaactac
     1501 tacgccacca gtttcatcta taaatccctg aggtaaaatt gccgctaaaa tccttctgtc
     1561 ctgttccttc gatgttgtcc gctgaacaag cacacattca gattttgtca caaggaaacc
     1621 ctgttgtggg gttatatcga tatagggaac atgatttttt ctgtctgcac taaaacgaaa
     1681 accagctgaa gttattgatt ccgcccagac taatgggtag ctattttttc ctttcgtcgt
     1741 acgcaactgc gacttaaatc gattccagac taactgacct gtagagactg agtatcccag
     1801 atcagctaaa cgcgtagaca tctgttttaa tacattaaga aaacaggcat catctttaac
     1861 gcgtggcaaa agccacgttg ctccaccttt ttctatatca acattaccta ttttctcaat
     1921 ttttgctttg ttcaacccct tagggacaag tgaggatacc tgggcccgaa ctttatgttc
     1981 tcccgccttg aatgctgtca acattgtttc ctggagaaca tcgtcaaaga ccccatccct
     2041 gtcagtgacg aaatcaaggg catacggagt agttttttcg gtcaaaaggg ttcgcagcgc
     2101 ggtgaaatac tgcccaccaa gaaaagaagt tggcgtaaga aatgcaataa ctcctgtttt
     2161 ctctttaacc atgcgaacag ctaagtcggt gaacaatcca tatagatttg catgaccaaa
     2221 taacgaacga gaatattttt ctctcgtttt aatatcgaga gtgacacgtc cgtaaggcgg
     2281 attccctacc acaagatcat aaccagaaaa tttatcttgc tgaagcgcat cagcaacaat
     2341 aattgtatct tcaggcaatc tacgttttac ctttacacat agtggcatta gtacagattc
     2401 gagcaatacc aaactcatcc aagccgcaaa tggatcgatc tctatcccct ttaaacgacg
     2461 actgattctc ctcaacatcc attcagggga agaccccttg tcttttttta acattcgaat
     2521 ggctactggt gccaaaaatg caccgccacc acacgcagga tcaataaccg atgcatgaga
     2581 aaaatcaaca ccagactttt cagccaggtc caagagacgc gctaccaaag gtgggggggt
     2641 atagtaagcc cccaattccg aacggtaagc ggatggcagc attaccgtat aaattgaccc
     2701 aatcagatat ccagcgtcct ccgccggaaa ttgagcaata agctcacctg ttcgttcagc
     2761 gagctcgcgt gcatcacatg gaatgtcttc tatgtaaaca gtgtgcggta ctgccctcat
     2821 tttaatgctg cttttttgct tttttgcaag ggtatcccaa tatgcagcta cgatgctgaa
     2881 gcagtatgag cgggcatgca tcaaccttga ctgcccggtt ttgtaattgc ccgcataacc
     2941 tttggccatg gctttacaag tttgatatct ttccagatta gaaaactcat tatctgtaaa
     3001 aaaatcaaaa tgttgcacat tcataatgta aaatcctaat gtatcaaaca tgcgggccat
     3061 aacatctcat ggtggcgcat agcaaactgg cccgaagaat atcacgcacc aagtcgatct
     3121 cataacccgt tatgacaata aatctttgta gctgaactca cttggtgatt tgtcaatcat
     3181 tcctggcctc cagttcgtcc tgaatctctt tatcgagttc accgtatttg ttagtcctca
     3241 tcactcacga cttgacttcc agaaataacc ttgattattc ggggctatat tttgtcaccc
     3301 tgcgtatccg cgctttcgca ttacgctcaa tctggattag cttttctata ttttttcgcc
     3361 tttcccgctc ctcctggcgc aagagcctta catcatctgc cagtctggtt tctcttttcg
     3421 ccacagagag catccagtca aatggctcca caactgcacc gcaaatttta cagcggacct
     3481 gacgctcttt ttcgtcaacc cggacagaag cgtgatggca atatggtctt tccgatggct
     3541 cataaagaaa attaacctga ttacgagggt tatcctcttt taccggaaat aaaacaatat
     3601 tgcttaactc atcttctggt tttatttcca tgctcctctc ctttgatgcg aatgccagcg
     3661 gtaattgaag cctgatagct aatttcactc acagtaccgc ctcctgaaaa tcaccctgat
     3721 agaaagccgg tacacgctgc ataacttcac tcttccggca ctcgcgacag attatgttct
     3781 gatgtctgtc gtagcggcgt atttctccgt ccggtaatga ccagataagg tccggatcaa
     3841 ccacagatgg tttcttcagc tttgccctcg agagtttttt gcgggcgttt tgccagtcct
     3901 tacgagcctg ttcagacggg aataacccgt agccagagtt gtatacatcc ccactggcaa
     3961 ccagctctct ggcgagaacg cttattaaat atcttgtcgc cccggtttta gcttccagtt
     4021 gtcgtaacgt ctcgcgccca ctctggcgta cgagttcaac aacctgcccc ttaatttttt
     4081 cccgctcttc ctgtgtaaat acttttgcca taagcgcccc cggcaatcac ttttccgaca
     4141 caatacgacc ggaggaatcg acaatctgtc gaacaatatc ccggtgcttg ttcagctccc
     4201 gcaacgcggc gcagacacgc tcccacttct ggacatgact tttcgcccga cgcagttcgc
     4261 ggtttgccat atgcagcgat ggtaaaacca ggtcatccgc ttgcgtttcg gtgaacgatg
     4321 gcaacgactg cacaatgtcc gccacagttt ctgttttaat ttcttcctgt gttgcagctt
     4381 cctgtaccgg taacgcaaca cctgctggct gaggaaagac tttaccatcc gtttccgcta
     4441 tggatgcggc tttcggctct gccggtaaat cagcgcccgg tatgcagtac cgaaatttac
     4501 cgccctgatt cacgcgaatc agacgccctt tgctgattgc catggccagc gatgaattcg
     4561 cccggcggga ggtaatcccg aacatcagtg ccagctcatc cgccgtttgt gggccatgtt
     4621 gttcaatcgc ctcagtcagc atttgcgctg tcactttcgg taccggtgac actggttcac
     4681 tttcaccagc ctgaatcagc caccacatcg aacccttgtt atccgcttca ccacggcgct
     4741 tcagtttcca cagttcgttg accgcatctt cacggctgat tccaaggcgg gacgccacca
     4801 catgtgaaga ggctcttttc agtgctttca gtgcgtcaaa tacagtttcc attaaaacat
     4861 cctccaacaa aaattacttc acaactcccg gaaagctgac atttgaacgc cagctatccc
     4921 agttaaacgt cacccaccga caaccgttca tggtcatgcg gtccataatc ctctcaccaa
     4981 gaagcgtgct cattgcggca tgattcaggt ttgttaacat cccgacactg cacagtgatg
     5041 ctgtccggcg atcaattatc tggtgcaata ccacctgctc gtttttcgtc tcccgctgaa
     5101 tgcctatttc atccaggacc agcaaatcaa ccccgcaaag ctcctgtaaa aatttttcac
     5161 cggatttgcc gttgtcgtag ctgtcatgca acacgctcat gacatcagac tcggtgacga
     5221 taatcacgct gcgccccttc gccatcagcc ggttacccat cgccgctgca aggtgatttt
     5281 tcccggtgcc ggttttaccg ctgaacacaa aattcgtgca cccggtcatc agttcgtcag
     5341 ctatggattt ggcctggctc agcgcgtatt tttgcccgtc gttctgcacc tgataattcg
     5401 caaacgagca tttgctgtgc agaggctgga tgcccgaacg attcaggatt ttttccaccc
     5461 gcaactggcg attctggcgg ttaatctcct cgctgcgttt tcgtccttca gcaagttgcc
     5521 attcccgcca ctcctccacc gtccggtacg gtggaaccgc cccctgtggt gcaagtctgt
     5581 gaatacgttc aagaaccccg gctgccgcaa tgtttttcat gccacatcac cccctgaatc
     5641 ccggcggaat ttcggtatcc ggttcagaaa tatgattcac acaacgctgg ttgttcgtgc
     5701 cgcttaccgg gagcaaccag gggttttcaa aattccggtc cggcccaaaa aacgtcgtcg
     5761 ctcgctgaac aaattccgtt cccgctttcc cggtcgccgc caggtatctc gcgtaacgcc
     5821 tcacaccatc cagcatggtc tctggtggca ccccctcgcg caatctggcc ttccaggcac
     5881 tgaatgcgga tttcttcggg tttgccccgg cacgcaacgg gtattcccgc cagacctgtt
     5941 cgaacacatc cggataatcc actcgtccca caggctgccc ggtgttttcc ggggctaccc
     6001 gatcggcttc ccgctgaatg gcggaatcgg cttcaggctg ctgcagttgg agtgattgct
     6061 ccggccttgc ggtcatcacc tgctgcacag cgcccgaatc ggctttcagc gcatacgctg
     6121 aatcggcttc cggtgtcgag cctgctggct gaccaggatt gacggtctga acatcccctg
     6181 cctggttcgt ggcgtttttt acgccatgga ccatagtgtt ttgatcttct ttatctgtat
     6241 ctttatctgt atctgtcgtg actcgtcgtg acatgtgcgt gacatttcgt gacgcgccgt
     6301 gacaatcgcc attttgttcc cgctttcttt ccctctctcg ctgcgccctc ttgcgctctg
     6361 caggagattt tgcggtttgc gaaatattgc cgttgtcctc tttaagcacc tggcgttttt
     6421 cccatccagt gattaaatca ccatcaagta cccgcccctg catcgtctgc aaaattgaat
     6481 caattacctc ttctgtcacg tcgagcgcac ttgccaaatc ttctgtcgtg acatcaatgt
     6541 gacctcgcgt gacatttcgt gacgcgctca ccaggaggtg gatatacact gccatcactg
     6601 ttgcaattgg ctgccctgac accctggcaa ttgttcgcca cttagggtca tttggcatgt
     6661 catgccataa tctgagccag gcgttagcca tactcacctc ttctgatacc gaatcttttt
     6721 actcacgagt tgccgggagc gattcgatat ggctattgtc agtcaatgta ctgccacagc
     6781 atttcctgcc gggccaccac ggttcatctg attgaaaccg gcgattgcca ctgcgacaaa
     6841 atcatcagcg tctctcacca gtcgttcatg cgtctccacc agttcccgaa aataagctga
     6901 actgtggctg cgcattcgag ccaccagcgg aggtggcatt gccctttcga tcgcaggtaa
     6961 caacgcctga attttttcaa cagcgtcagg ggtgtcttta tccagccaac ggaaaatctt
     7021 ttgggtgttg agataaagcg tgttgtgctt gctgtcgtta tgcagaaccg gaaacgtcat
     7081 tcccagttcg aaataaaccc tggttatttc agctgccgga actttttcac cgtccggatg
     7141 cgcccaggct ttcatcgcca tgcggatgtg ttcatgcttg attttcatga atcaaccccc
     7201 gccgatggtt gtgtgttagc ctgaaactca gcaggcaagc cgtcagttgg gttggggtag
     7261 gttttactgt caatctcatg tggggtcacc acccatcccg ttgcttcaca ccaacgtaat
     7321 attttttctc ctgtaagttt tgcacgcccc gttataacgt ggctgaccat cccttgggat
     7381 gcgccaacaa tctcagcaaa tgaactctgg gttatatgaa aatgatgcaa atattctcca
     7441 agattcataa tccgccctat gtgatgtgtt accaatgcat aattaatag
//
LOCUS       DAEEKQ010000186         7403 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.188, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000186 DAEEKQ010000000
VERSION     DAEEKQ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.188"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(107..4518)
                     /locus_tag="KLK57_003644"
                     /pseudo
     CDS             complement(107..4518)
                     /locus_tag="KLK57_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289076.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR/RHS domain-containing protein"
     gene            complement(4538..4999)
                     /locus_tag="KLK57_003645"
     CDS             complement(4538..4999)
                     /locus_tag="KLK57_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
                     /protein_id="HBE0893913.1"
                     /translation="MQFTFNEGHIQLPSQWQDQSMQVLVSTDNSGINLVITREAVPQG
                     TLTPELYQETLALYQGKLDGYTEHACREITLAEAPAWLLDYSWNGPEDEGNQGRISQI
                     AVFQRRGDTLLTFTFSTSLSLKNSQKTMLLEVIKSFTPLPPENDIQKDQPR"
     gene            complement(5027..6928)
                     /locus_tag="KLK57_003646"
     CDS             complement(5027..6928)
                     /locus_tag="KLK57_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBE0893914.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFEQEGQHQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAETARGMSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     QGAGTALENHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRPPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDSIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT         1428 a         2230 c         1979 g         1766 t
ORIGIN      
        1 ttatttgact acgtgataaa cgaaccccat ccaggaaacc cgtatgccca ctaacacgac
       61 gaccatcgcc aatatagtcc atcaaactgc tgattttaga acttgcagcc aatcccagcg
      121 gatcaaccca cccacaagga ttcggcacat accggtaagt ctgctccccg ccctgcaacc
      181 ccagcggatc actcaccagg tacattccgg tttccggctc gtaataccgg aaccgattat
      241 agcacagccc ggattccgca tcctgccact gtcctgcata cagcaggccg gggtcggctt
      301 ccgggtccag ttccccgcgc gggtccgggt agccggtgtc tgcgggcagg ctgagtgcca
      361 gcccccacag gctggtctgc cccggtcgcc agaccgtctt tccttcactg ttaaagagca
      421 tcagcgggcg gcccgtcagg tcgctcaccg catgattcgt ccgcgtcacc cactgcgggg
      481 ccaccgatgg atgcgcctgc cgtacccggc tgaactgctg gcttatcagc tcaaagccgt
      541 taaacaccag gtgccttacg ctgtacagtt tatcatcgcg gtattcccgg atttccgcaa
      601 tactgtcgcc gtcccacaga tacgtgaagc ggattttctt ccggtcgcag cgtttctctg
      661 ttcgtctgcc gctggcgtcg tggcggtatt cccacagctc accctgtgcg ctgcaatacc
      721 cggtcagctg gtcccggctg tcccaccgga accgctctgt ttccggtcgg tagccgtcac
      781 ggtgtctggt gcggctgacc atccggcctg cggcgtcata gtcatactgc gtgtttccgg
      841 cctgtttcag ccggtggcct ccggcatact ggtcagtctc ttcacttatc cggtgcctgc
      901 ccgcagactg cgccttcagg tagccgcagc tgtcatagcc ataggactca tcgccttcgc
      961 catagcccag cccggtgcct gaggctgtca ccgccgtgac ctggccgttc cggtccagcc
     1021 gatactcccg ccgtgtttcc cgtccgtaat cttcccggct ggtgacgcct gacacgttac
     1081 ccgcgctgtc gtaacggtac tcccgtacca gtgagggaac cagcccgtcc catgtggctt
     1141 ctgccggaaa aaatccggcg cgctgactga caggctgacc ggctgcattc cagccctgtt
     1201 cacaggcaaa accccggtta ctgtgccgga gcacttcacg tcccgcctca tcacggctga
     1261 acgtcagcgt ctcatccccc ggcagaatga cctctgtcag ttctccggcg gcgttgtagc
     1321 ggtacgtggt tttcagcagg gattcaccgg ggtgtctgtc gcgttcatct tccggtggca
     1381 ggatacagcg gatgacggtg tgttcatcgg ggtagttgta ttcaatggcc cgtcggccct
     1441 gagtggtgac attcacccgc ccgcgttcgt catagccggt gcgctgtgat gtgccgtccg
     1501 ggctgaagat acccgtcagc ctgccggatt tgtcataggc atactgcgtt ttgccttccg
     1561 gcgagatgat ttctgccagg cggcctgcgg cgtcccggct gtagaggatg gtttcctcca
     1621 ggccgttaac cttggcggta cagaggccat cggcgtcata gcggtaacgc cagaccacgc
     1681 cgccccagtc acgctcttcc gtcacccggc cgcagttatc cagaacataa agatatgact
     1741 caccctgcgg gttgatgact tctgtcagtt gcagcgtgtc tttatcgtag cggtactgcc
     1801 agcggtggcc ttcaccgtcc gttcgcgcca ccggaaaatc gaacggaccg tattccatcg
     1861 tccagacaac accgttgccg tccgtccagg ccagcaggtt gccgtggcgg tcatagcgaa
     1921 atccttccgc gccggttcca gcacgttcaa tgccgctcag tttatggtgc tgctgccact
     1981 gtaaccgcac tgactgaccg ccggggccgc gcatcttcac tggcaggtcg tgaccgctgt
     2041 attcggtgtg gtgttcacgg cccatcacgt cggtgagtgt ttccggccgg ttgtggtggt
     2101 cgtactctat gcccagacgc cgttcatcct ggcggaatat tcccgacagc agcccctgag
     2161 cggtgtatgc cagccgggtc tcttcgccct gtggcgacac cagacttacc ggcaggccgc
     2221 gttcatcaca ctgctgtgtc cacgtctgcc cggcagggtc ggtgatgttg ctgagcaggc
     2281 cgcgctcatc atagcggtac tgccactccg cgccgtctgc cgccgtgtaa cggcttaact
     2341 gaccgtgtgc ggcgttgtat tcaaatttct ccacccgccc ggcaggggag tggcgggcca
     2401 gcaggtgatg gaattcatcc cattcataca tggtggtgct gccgtccggc gcttcttccc
     2461 gcagaatatt gccgtcagga tcatagtaat aacggtgaat gcccttgccg tccgtcacgg
     2521 tggtgcagcc gtcaccatag tccagcgtgg cgttgtaata gccctccgcc ccggtgacat
     2581 tcacgcaccg gccctgttca tcgtaggtga aacggctcca cgtctggtcg ttatcggacc
     2641 agcggatgat ccggtccgca gcgtcgtact cataaaacag gtggtagtcc agccgcgcat
     2701 ccgcttctgt cagccgcccc cgcgcatcct gttcgtaggt cgccagcgtc tgtatgtcgt
     2761 tatccgtgcg gtgaatggct ttcaggtagc cgctggcgtg gtacgtcagt ttaagccgga
     2821 tcccgtcggt gtgactgaca cccgtgatcc ggtactggct gtcgcgctca aacgtgatgc
     2881 ggttgctggc ggtgtcgctc atcccgccca gcatgtacat ccggtcattg gggctgtgtg
     2941 ccaggcggtc atcgctggta tcgtcagtca ccgggtggcg cagacgcatt cccggaaagg
     3001 cgtcataaaa gaagcggctg ctcagtgcat ccctgtcgaa cagctccaga taatcatcgc
     3061 ggcggcgcag aatgtactgg gcgtgatacg ggttaaccgc cgtatcactg tcaccgtcga
     3121 aggcaaatct cagcgtggct ccctggctga tgatatccac ccggtttccc tgttcacgca
     3181 cccaggcgta ttcgctccag gagtcgctcc agcccacgcc ggtcaggcca tgcagcggca
     3241 gtacagaact gtggaaacgg gtgaaggaga gggggagggt ctggcccagg gtgaagtcgg
     3301 tacgttcgtc gcagtatgcg ccggttaccg gatcgacagg atctgttacc catctgatga
     3361 ctttattggc aatacttttt aacgacttac ctgttttccc cagcaacgcg ccgagctttg
     3421 cgcccagcag cttcccgctt ttccccagca gtttcccagt ttgccgagtc tcgccagatt
     3481 cttcagtttt cccagtagtc ccgctggcgg cagaagatag ctggctatcg ttattccccc
     3541 caggatcatt ctctgccacg gcgggaactc cggctggata tccagatatg tgacctgctc
     3601 accgccaata aagacgtccg gcgaacctcc cacgatcgcc gcactgcact ccagtttgtc
     3661 gcccttgcgc gcggcaggct gattgttgat aaagacgttg ctgctgccct gggctatcct
     3721 cggtggtttg ttctccttgt cgcagagcgc cgtgtcctca ccggcttccg ggaaatccac
     3781 atcttttttg atttcgccac gcccgcccac catggtggca aactccatta ataccggacc
     3841 ggcgggaaac agaagcgatg cgccggcgct gacgcgatcc ataaaaccgg cattgctgtc
     3901 cgtcagggtg ataacagcgt tcccccaggc cttcgccagt ggtactgctt tttccgctgc
     3961 cgattcaagc aggtcagtgg caatatcacc ccatgagggt tccccctgtg ggacttcagg
     4021 tattgctcca ggtgggggag gagggggaag ggtgaccgct gcacgtgcgg ccagctcgcc
     4081 gttaattttt acgttttcag aaccggtatt gatggcgccg tccgtgtcac aaaagctgtt
     4141 tatccatgat tcaagctggt tctgtttctc ctccacataa tcactcgcct tatagctggc
     4201 atacatcagc gcaggtacca gaaccagcgc ataagggccg gtggcacagg cggccataat
     4261 caggcaacct tctgcgatgg tcactatcgc ccccagtacg gcccctgcca gcgcccccaa
     4321 aaaactcttg tgctgaattt catcgcccag cctggcggcg ggggggccag acttttttga
     4381 tgccaacatc tgtgcaatgg ctttgtcgtt ggctgcccgt ttcatctggc tggcgaccat
     4441 cgcctgctgt gacagcattc gcattgccag cgtaccgcct gcggtcgctc cctgtggccc
     4501 gcctggtcct tcactcatgt gttatccctc gtttatgtca gcgcggctgg tccttttgga
     4561 tgtcattctc cggtggcaac ggggtgaagc tctttatcac ctccagcagc atcgtctttt
     4621 gactgttctt cagggataag gaggtgctga aagtgaaggt cagcagcgta tctccacgcc
     4681 gctggaatac ggctatctgg ctaatccgcc cctgattccc ctcatcttcc ggaccgttcc
     4741 aggaataatc cagcagccag gccggggcct ctgccagagt gatttcccgg caggcatgtt
     4801 cggtgtaccc atcaagtttg ccctggtaca gcgccagcgt ttcctggtac agctctggtg
     4861 tcagggttcc ctgtggaacg gcttcgcggg taatgaccag gttgatgcca ctgttatctg
     4921 tggacaccag gacctgcatt gactgatcct gccattgaga cggcagttga atgtggcctt
     4981 cattaaaagt gaactgcata aaatactccc tgataatctg agtgaatcag ttgatgaaaa
     5041 cagaaccgcc cacgatggtg atgccatcct tcgacagcct gattaccgtc gattccccga
     5101 cctgtagctg aatgctatcc ggcgtcagcg caattttgct ttcctccacg gccagcaaaa
     5161 tttccttaat cgccgtataa gcctgtatac cgtcgccttc gccaccattg tttacagcgt
     5221 caacatgcac cgaaccggca atcgtgcctc tgtcctgaaa gacatcctca gataaagacc
     5281 ctttcagtac tccggtgatc ttgtggcctt tttccaccgt aagaaggcgg ttaccttcct
     5341 ggatcgtgac cgtctggtca ttttttacgg tttcgcggtg atcgtgtttt acggtggtgg
     5401 tacggtcgtt gagcacttcg gtatccatgt tcttctgcgc gtggatgtag acctgttctc
     5461 tccctgtcgc atcatcaaac ttcagctcat taaatccgct gcccatgtac gttttggagc
     5521 ggatggtcat ctgcgtcttt gttcccggca ggctgccggg ggggcggttt tcctggtggt
     5581 aggtgcgccc cataatgatc ggctggtccg gatcgccgtt gaggaagtct acaatcactt
     5641 cctgacccac acgcggtatc gccaggtggc caaaaccggt gcctgcccac gcctgtgcca
     5701 cacggatcca gcacgaactg tcttggtctg ccgggttata acggtcccag ttgaacttca
     5761 cccgcacgcg accatgttcg tcgcagaaga tttcctcgcc tgccggtcct gtcacgacag
     5821 cgctctgcgg gccgtcgacc agcggtttca gcaacggctg tggtcgccat gttctgtctg
     5881 ccgggatcac cgcaaaatgg ttctccagcg ccgtccccgc tccctgccgt cctggcaccg
     5941 cctgtggctg ttcgccgtgc agttcacttg ccaccacctg ccattcccgg ttcaggttcg
     6001 cctgcggatg ccccgtcagc acaattcgtc gtcccggcca tatctccggc gagcgactca
     6061 ttccccgcgc ggtttctgca ttgtttcgcc agccgtccat ctgccagcgg gcaaagttct
     6121 gcccgtgagc acccttgaaa cgtccggggt agtcatacac ttcatactgc gtgcgctggt
     6181 agtcctggtg ctggccttcc tgttcaaaac gtccggccca gccggggcgt ttaaaggtgt
     6241 agtctttggt caccacggaa gaagggcgga tttgtgcgct gtagcggaac tggctgatgc
     6301 agagggtgct cacctcggta cgggtgttcg ggttccatgg gatttcaaaa gattcgggca
     6361 gatggcggac tgtgtcgcac agcaccaggc tctggtcggt acttttgtaa gcatgctcct
     6421 cataaaagaa gatgccttcc tccgccgcca tccggcacag gaaatcgtaa tcagtctcac
     6481 cgtactggac acaaaactca cgggaaggat gcggctcgct gaacagcgga ctccattcgg
     6541 tcaccccgtt ttcctgcaac atcgtgccga ggatgctttt gatgtcctcg ttctggaaga
     6601 tacggaagtt ctggcgcaga ccggcacgcc acagcggcgg gtgcaccttc atgctgtaca
     6661 gcatctggtt tttgtcgttc tccccgagtt caaaccaggt caccacgcct ttcacccggc
     6721 gctgtacttc atcgccctgc catatcgtca ggtaggccat tttgtccagc acctgcgcaa
     6781 attcaaggga gagaaactgc tggctgacca gggagagatc gagggaaaag agcgaagaga
     6841 gtgactggtt cagatgaaag gaaaccaccg caaaagcgtc cggcggcagg ccgtccactt
     6901 ccagtgtgaa acgtaatccg gttgacatag caacctccag taaaatgaat aatgatccgt
     6961 accgtacaac tgcggataat gtgataagcg ggacgacttc agacgttatt agggaaggaa
     7021 cagaaaatgc tatggttatg tgagaatttt gtttaaatat tactttacag aaacgggtgt
     7081 ctgttcagtc aaaaaaataa tcacaagctc aacctcttgt ttcttggcgt tattcaggac
     7141 aggtacgcat atcacaacga gtaacaaaaa cgcaaagtta aaccaatatt cataatatat
     7201 ttttattcac agcaataaat aatccaatta acattattaa aatgaatgat tgcataagaa
     7261 atatatgcta ccgctccacc ctgataacat gatctgcaca ttgcacatct taacgttgtt
     7321 aaatactggc tcaatagtgg atattttaaa ttaggaattt atcaaattca cttgacaaat
     7381 agattgagtg aataatataa caa
//
LOCUS       DAEEKQ010000187         7249 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.189, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000187 DAEEKQ010000000
VERSION     DAEEKQ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7249
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.189"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..146)
                     /locus_tag="KLK57_003647"
     CDS             complement(<1..146)
                     /locus_tag="KLK57_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HBE0893915.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEP"
     gene            complement(339..1529)
                     /gene="glpC"
                     /locus_tag="KLK57_003648"
     CDS             complement(339..1529)
                     /gene="glpC"
                     /locus_tag="KLK57_003648"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HBE0893916.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGIGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(1526..2785)
                     /gene="glpB"
                     /locus_tag="KLK57_003649"
     CDS             complement(1526..2785)
                     /gene="glpB"
                     /locus_tag="KLK57_003649"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HBE0893917.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(2775..4403)
                     /gene="glpA"
                     /locus_tag="KLK57_003650"
     CDS             complement(2775..4403)
                     /gene="glpA"
                     /locus_tag="KLK57_003650"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HBE0893918.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            4676..6034
                     /gene="glpT"
                     /locus_tag="KLK57_003651"
     CDS             4676..6034
                     /gene="glpT"
                     /locus_tag="KLK57_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HBE0893919.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            6039..7115
                     /gene="glpQ"
                     /locus_tag="KLK57_003652"
     CDS             6039..7115
                     /gene="glpQ"
                     /locus_tag="KLK57_003652"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HBE0893920.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRGHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMDLNLVQLIAYTDWNETQQKQPDGSWVNYSYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7157..>7249)
                     /locus_tag="KLK57_003653"
     CDS             complement(7157..>7249)
                     /locus_tag="KLK57_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0893921.1"
                     /translation="SLLAKEKPVEGSPLFLLQMCMEQLVAIGKI"
BASE COUNT         1657 a         1822 c         1989 g         1781 t
ORIGIN      
        1 ggttccaggc gtaaggtttg caggttgcaa agcttgcgca gtggttctgg caaatgtatt
       61 tcgagaaaat cccgtgcggt ttcgggtgtg aacataaagg ttttaaatac cgcatcatgc
      121 ggggaggagg ttgttgattc ggtcatggtt gtccgtcacc tgatatttat gatgacgcga
      181 cattacggtt atcccaggcc tttgacatct gccagtttca gcctttgcga gacgttttca
      241 gaggaatttt cactgcgttg caacgcatac attgggcacg gaatcgtgta cgcaaaaatg
      301 cccggtaacc acatgcttac cgggcatcag aaaggagttt aagccagcgc ctgggccagt
      361 agcgtaatcg gatgttcgca gcgaagactt gtggacatct caatctgcca tttacaggtt
      421 tcgcagtcgg tgaccaccag atctgcgccg ctttcttcta tctggcggaa cagtggtgcg
      481 ccgatggctt gtgaggtggg gtagttctct tttttgaaac cgtaagtacc cgcaataccg
      541 cagcactggg aatccagcac cgttaactca agccccggga ttttacgcaa cagctccagg
      601 gtgtagagcg tccagcccat tttttccata tggcacggag tgtgataaac cactttcagc
      661 ggcagcggtt tcagcggtaa cgttttgcct tcgtccagct tgcgccacag ccagcgggtt
      721 gccagttcga tatgatcgcg caagcctttg ttgtcgacat tcagcacttc cgggtattcg
      781 tcgcgcaggg caaatgtaca ggttgaggag gtggcaatca ccggaatgcc ttttactccc
      841 acagcttcgc ggatcgactc tacattcgta attgcctgtt tgcgtgcttt atcggtaaag
      901 ccgttggcga ttagcggtac gccgcagcat ttttctttgc tgagcagttg tacaccgata
      961 cccattgcgt tgagcacttt aattaaatct ttacctaact gcggatggtt gtagttaacg
     1021 aagcagccgt gaaagaaagc gacctggtct ttatattgtg cttgctgagc cgctatgctg
     1081 cgataccagc gacggaacgt gccgaaggag tatttcggta gcgtgcgacg atgatcgatt
     1141 tttaacgccg catcaagcag ctgccgcacc ggtttcagcg aggtagcggt gttgacgatt
     1201 ggtgcgaacg gcgtggaaac gctacccatc aggtcggtat gactcaacac aaaattacgc
     1261 agcgacgggc gcgtggtgtc atattttgcc cgcgcgcgct ggataatatc gccaatcttc
     1321 acatcggacg ggcaggcgac ttcacaacgt ttgcagttga tgcaatattt cagcgcctcg
     1381 tcatacagtg cgccatcttt caaacgcaga cgctcgccat ccggcccggc ttgttttggc
     1441 cctggataac cgggattcac ccggctcacc gggcaggcgg tggtgcagac ggtgcactta
     1501 atgcagtttt cgaagctggt gtcgttcatt gttggcctcc tgcgcgttgg gcaatctgtt
     1561 gagcggcatg taaagcactg acggcacaaa caccgccgcc gcatccttgg gcgatgggat
     1621 caaatccgcc cagcaccgaa ccgatggcaa acaggttctc aatggtttgc cctgcctgcg
     1681 acgggcgtag cgtctcatca gtggttacac cgaactgctg ccacggttgc ggcgcaaaaa
     1741 aatcgccctt ataccattca ccccgcgtgg cggtttgtag cacatcaagg ccgagaatcg
     1801 gctcgcgaat gccgttacgt tccgctacca gtccgccact aaagaaactg ccgctggcga
     1861 gaaccgcgaa acgtggacgt agcggaatat cggcgtgatt gcgggtccag atttcattca
     1921 ctacgccatt tttacaggtc acttttttca cttcatcgcc cggcatccac acgccaccct
     1981 ggcgcacaaa ctggcgctgt aactggtttt gcagacgaat gcccagcacg gaaggcggca
     2041 gcgttggcaa aagcatcagt gaacaaggta gtttttcatt caaccaacgc cacagtttgt
     2101 catcggccag gccgaagcag gcgggcatca ggatcatttc gcaggtattg gcgacaggaa
     2161 taagcgcatc aagtaacagc ggccagtttt cttcattatc aaggaaacgg gcgatattca
     2221 ccgcgcgaaa ttcggtggcg ttattgcgca ggacatccag ttccggcagc tctatttctg
     2281 cggtttcaac cgaaagatcc agttcgcgca acgaagccgc cgcaagatgc gcctggaaat
     2341 ccatcagccc gcttatcccc actacacata tcttcttcgc gggtagcggc cagacgggaa
     2401 cttctggcga acttaaccag gttgcacgca aagtgccgag cggggtaacg cgctgatggg
     2461 ccagttccac gctgccctgc aattgcgcac cgctttcagc gatcaatgcc tgcgcctggc
     2521 aagcgagatc gagcacgcgt tgtggttcga gaagactgta aggatgggct ggtgcctgct
     2581 gacgcaaaga ttccagtcca ctgtgaatgt ctgtcaccgg ttgaccatca ggcagatggc
     2641 tcagcaaatc cagcgaaccg gatgagaaat gcagcgcgct ttgaccacgc gtgacaatgg
     2701 cacagcgcag gccgtgtttt tgcagttgca ggccacagag taatccggcg aggccgccgc
     2761 ccataatgac agtatcaaag cgcatctttc tgctccttct ccagaccaca cagtccctga
     2821 taaacccagc gggtaaattc gctttcgcgc agtgcatctc cccaggcgat gggttgcacg
     2881 cctttccagc gttcgttaag gaaggtggaa agttgctcga tagattgcgc ggacgtcgtg
     2941 acgttaaaac gttgcagcag tccggcagcg cggcaggcgc agagttcgcc ctggcaggta
     3001 cccatcccca cacgggtacg acggcgtaaa tccagcaggc tattaacgtt taaattttct
     3061 accgcgtact gcacttcacc cgctgttacc gcttcgcact cacataccag gctacggtgc
     3121 agacgacctt cgctcagcca ggcaggcgtg cgatcgccat gacgataaac cgcagaaccg
     3181 cgcagcgggg caggcaggga gatgacttta cgcaaggtaa cttcagcggg ttcctgtgaa
     3241 ccaggaagtg ccagatcggc ggtcgtacag gggcgcgtgt tgcccagttt gcggcacacg
     3301 gcgtcggtag cccattcagc catcagccga taggtcatca gtttgccacc ggtgatggtg
     3361 ataaatccgt ccagaccatc gcgttcagca tggtcgagca gcacgatgcc acggctgacg
     3421 ttacgtccgc tcgggtcgtc atcgctggca accagcgggc gcacgccaga ataggcccgc
     3481 aaaatgcgcg ttttcgccat cacgggggcc agtttttccc cttcacgcag cagaatatca
     3541 acctcttctg ccgtcactcg attatcgtca atctcgttgt aatcaatacg taaagaggtg
     3601 gtgccaatca gcgaaatggt atcgccaggc accagaatat cggcgtcgga aggtttacgg
     3661 cagcggttga tcacatgctg gttaatgcgg tgatccatga tcagcagcga tcctttcgcc
     3721 gggaacatgc gaatgcgcag atcggcatat tcggcaatgt gttgccccca gatcccggcg
     3781 gcattaacca cgacaggcgc atgaagggcc tgagtttcgc cggtgagatg gttacgtaca
     3841 cgaacaccgc acaccgtcgc gccttcacga atcagtcccg tgacttcatg agcggtaagg
     3901 ataacggcac cgtgttcttt ggcatccagc atgtttgctg cggtcagacg aaatggatca
     3961 acggtgccat ccggaacttt caccgcgcca atcaatgccg ggttaacggc aggttcgata
     4021 atgcgcgctt gctgcgggtc tatagcttct gcgctgatcc ctgcttcttc gcaggcgcga
     4081 ataaaagtgg cctggaagga gaggtcatct tccggcaggg taataaacag gccgttggtt
     4141 ggttcaacgc agtgacgtgc aatgcgtttc aggatctggt tttcactaat gcattcgcgg
     4201 gccgattccg catcggttac cgcatagcgc gcaccgctgt gtagcaggcc gtggttacgc
     4261 ccggtggcac cggttgcgat gtcgtggcgc tcaaccaaaa tcacgcgcag cccgcgcagg
     4321 gcacagtcgc gggcaatccc ggctcccgtt gcgccgccgc caatgataat cacgtcactt
     4381 gattgcgagt cgcgagtttt cattgttatc cctctgaagt tcgtttttta ccatttagcc
     4441 atagtaaaaa catgaattgt ttgatttcgc gcatattcgc tcatgattcg aaagtgaagc
     4501 gtgatttcat gcgtcatttt gaacattttg taaatcttat ttaataatgt gtgcggcaat
     4561 tcacatttaa tttatgaatg ttttcttaac atcgcggcaa ctcaagaaac ggcaggttct
     4621 ctcactgaat caggctgtta atcataaata agaccacggg ccacggaggc tatcaatgtt
     4681 gagtattttt aaaccagcgc cacacaaagc gcgcttacct gccgcggaga tcgatccgac
     4741 ttatcgtcga ttgcgctggc aaattttcct ggggatattc tttggctatg cggcttacta
     4801 tttggttcgt aagaactttg cgcttgctat gccttatctg gttgagcagg gattctcacg
     4861 cggtgattta ggttttgccc tttcggggat ctcgattgct tatggatttt cgaaattcat
     4921 catgggttcg gtatcggatc gctcgaatcc gcgcgttttc ctgcccgcag gtttgattct
     4981 ggcggcggca gtgatgttgt ttatgggctt tgtgccatgg gcgacgtcga gcattgcggt
     5041 gatgtttgta ctgttgttcc tctgcggttg gttccagggg atggggtggc cgccgtgtgg
     5101 tcgtactatg gtgcactggt ggtcgcagaa agaacgtggc ggcattgtgt cagtgtggaa
     5161 ctgtgcgcac aacgtcggtg gtggtattcc gccgctgctg ttcctgctgg ggatggcctg
     5221 gttcaatgac tggcatgcgg cgctctatat gcctgctttc tgcgccattc tggtggcatt
     5281 attcgccttt gcgatgatgc gcgatacccc gcaatcctgt ggcttgccgc cgatcgaaga
     5341 gtacaaaaat gattatccgg acgactataa cgaaaaagcg gaacaggagc tgacggcgaa
     5401 gcaaatcttc atgcagtacg tactgccgaa caaactgctg tggtatatcg ccatcgccaa
     5461 cgtgttcgtt tatctgctgc gttacggcat cctcgactgg tcaccgactt atctgaaaga
     5521 ggttaagcat ttcgcgctgg ataaatcctc ctgggcctac ttcctttatg aatatgcagg
     5581 tattccgggc actctgctgt gcggctggat gtcggataaa gtcttccgtg gcaaccgtgg
     5641 ggcaaccggc gttttcttta tgacactggt gaccatcgcg actatcgttt actggatgaa
     5701 cccggcaggt aacccaaccg tcgatatgat ttgtatgatt gttatcggct tcctgatcta
     5761 cggtcctgtg atgctgatcg gtctgcatgc gctggaactg gcaccgaaaa aagcggcagg
     5821 tacggcagcg ggctttaccg ggctgtttgg ttacctgggc ggttcggtgg cggcgagcgc
     5881 gattgttggc tacaccgtgg acttcttcgg ctgggatggc ggctttatgg taatgattgg
     5941 cggcagcatt ctggcggtta tcttgttgat tgttgtgatg attggcgaaa aacgtcgcca
     6001 tgaacaatta ctgcaaaaac gcaacggagg ctaatggcat gaaattgaag ctgaaaaacc
     6061 ttagcatggc gatcatgatg agcactatag tcatgggaag cagtgcaatg gcggcggaca
     6121 gcaacgaaaa aatagtcatc gcccatcgcg gtgccagtgg atatttgccg gagcatacgc
     6181 tgccagcaaa agcgatggcg tatgcgcagg gagcggatta tctggaacag gatttggtga
     6241 tgaccaaaga cgaccatctg gttgttctgc atgaccatta tctcgatcgt gttactgatg
     6301 ttgccgatcg tttcccggat cgggcgcgca aagacggtcg ttactacgcg atagatttca
     6361 cgctggatga aattaagtcg ctgaaattta ccgaaggttt cgatattgaa aacggtaaaa
     6421 aagtacagac ttatccgggg cgtttcccaa tgggtaagtc cgacttccgg gggcacacct
     6481 ttgaagaaga gattgaattt gttcaggggt taaatcactc taccgggaaa aatatcggta
     6541 tctatccaga aatcaaagcg ccgtggttcc atcatcagga agggaaggat attgcggcaa
     6601 aaacgctgga agtgctgaag aaatatggtt acaccggtaa ggacgataaa gtttatttgc
     6661 aatgttttga tgctgatgag ctgaagcgta ttaagaatga gctggaacct aaaatgggca
     6721 tggatctcaa tctggtacag ctgattgcct ataccgactg gaatgaaacg cagcagaaac
     6781 agccggacgg aagctgggtt aattacagct atgactggat gtttaagcca ggtgccatga
     6841 agcaagtcgc ggaatatgcc gacggtatcg ggccggatta ccatatgttg attgaggaaa
     6901 catcgcagcc gggtaatatc aaactcactg gcatggtgca agatgctcag cagaataaac
     6961 tggtagtgca tccttatacc gtgcggtcgg ataaactgcc tgaatacact actgatgtga
     7021 atcagttata tgatgctctg tataacaaag cgggtgtaaa tgggctgttt actgatttcc
     7081 ctgataaggc agtaaaattc cttaataaag agtaatgcta ctgcaggatg gcgttcatgc
     7141 gccatcctgt ctcatatcaa atttttccaa tcgccactaa ttgttccatg cacatttgta
     7201 gtaaaaacag tggactgcct tctaccggct tttctttagc caacaacga
//
LOCUS       DAEEKQ010000188         7239 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.190, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000188 DAEEKQ010000000
VERSION     DAEEKQ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7239
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.190"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..337
                     /locus_tag="KLK57_003654"
     CDS             <1..337
                     /locus_tag="KLK57_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBE0893922.1"
                     /translation="ADLSRYSGTVVNRPTFFAFAKGAGVMGEAGPEAILPLRRGADGK
                     LGGVADTGGSGMVMFAPQYNIEINNDGTNGQIGPAALKVVYDLGKKAAADFMQQQARD
                     GGRLSGAYR"
     gene            337..666
                     /locus_tag="KLK57_003655"
     CDS             337..666
                     /locus_tag="KLK57_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0893923.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVKIRVRKGEHPFLKAFLERHGGVRAFQWTPPYDWKPIRVVCRKWSASVGALWVTITA
                     DFEQVVA"
     gene            676..1374
                     /locus_tag="KLK57_003656"
     CDS             676..1374
                     /locus_tag="KLK57_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HBE0893924.1"
                     /translation="MQDIPQETHHETTRLTQSAQVVLWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGTGFELNGKGSAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            1379..2122
                     /locus_tag="KLK57_003657"
     CDS             1379..2122
                     /locus_tag="KLK57_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0893925.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGAIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRNGQNLYLDNMEVT
                     GFYRVALTEAQPGDVLLCCFGSSVPNHAAIYCGDGGLLHHIPEQLSKRERYTDKWQRR
                     THSLWRHRAWRASAFTGIYNDLVAASTFV"
     gene            2059..2661
                     /locus_tag="KLK57_003658"
     CDS             2059..2661
                     /locus_tag="KLK57_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HBE0893926.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTYFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            2722..6201
                     /locus_tag="KLK57_003659"
     CDS             2722..6201
                     /locus_tag="KLK57_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HBE0893927.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNLSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVTDGFGGTEPRITCNSYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQMLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNAVEVNWIDPDNGWETATELVEDTQAIARYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGSGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITAVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAIGRASDDAEGYLNFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKCLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKATAGITIS"
     gene            6269..6868
                     /locus_tag="KLK57_003660"
     CDS             6269..6868
                     /locus_tag="KLK57_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HBE0893928.1"
                     /translation="MRKVCAAILSAAICLAVSGAPAWASEHQSTLSAGYLQTHTDMPG
                     SDDLKGINVKYRYEFTDTLGLITSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     PSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDGGRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            6932..>7239
                     /locus_tag="KLK57_003661"
     CDS             6932..>7239
                     /locus_tag="KLK57_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBE0893929.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     "
BASE COUNT         1637 a         1727 c         2315 g         1560 t
ORIGIN      
        1 tgctgatttg agtcgctaca gtggcacggt ggttaaccgt ccgacgtttt ttgcttttgc
       61 aaaaggcgcg ggtgtgatgg gggaagcggg acctgaagcc attctgccac tgcgtcgcgg
      121 tgctgatggt aagctggggg gtgtggcgga tactggtggt tcaggcatgg tgatgtttgc
      181 cccgcagtac aacattgaga tcaataacga tggcacgaac gggcagatag gtccggctgc
      241 cctgaaggtg gtttatgacc tcgggaaaaa agcggccgcg gactttatgc aacagcaggc
      301 ccgtgatggt ggccggttaa gtggagcata ccggtaatgg agacgtttca ctggaaagtg
      361 cgcccggata tgaatgtggt atcagagccg aaagtggtga cagtgaagct gggcgatggt
      421 tatgaacagc gtcgtgcggc gggactgaat aaccagttgt cgacttacag cgtgaagata
      481 cgtgttcgta aaggtgaaca cccattttta aaagcctttc tggaacggca cggtggcgtc
      541 cgcgcatttc agtggacgcc accttatgac tggaagccga tcagggtggt ttgtcgtaaa
      601 tggtcggcaa gcgtgggggc gttgtgggta accataacgg cagattttga acaggtcgtg
      661 gcataggagg ctctgatgca ggatattcca caggaaacac atcatgagac gacacgcctc
      721 actcagtcag cccaggtggt gctctgggaa atcgatctga cagaggtcgg tggtgaacgt
      781 tattttttct gtaatgagca gaacgaaaaa ggtgagccgg ttacctggca ggggcggcag
      841 tatcaggcat accccattca ggggacggga tttgaactga acggcaaggg cagtgctgcc
      901 cgtccgacac tgacggtttc taacctgcac ggaatggtca ccgggatggc ggaagacctg
      961 cagagtctgg tcggcggaac ggtggtcagg cgtaaggttt acgcccgttt tctggatgcg
     1021 gtgaacttcg tcaacggaaa cagcgacgcc gatccggaac aggaggtgat cagccgctgg
     1081 cgcatcgagc agtgcagcga actgagcgcg gtcagtgcct cttttgtgct ggccacacca
     1141 acggagacgg atggcgcggt tttcccgggg cgtatcatgc tggcgaatac ctgtatgtgg
     1201 acttaccgtt ctgatgagtg tggttacacg ggcagggcag tggctgacga gttcgacaaa
     1261 ccaacgacgg atatccggaa ggacaaatgc agcaagtgta tgcgcgggtg tgagttgcgc
     1321 aacaataccg gtaatttcgg cggtttcctt tccatcaata aactttctca gtaaatccat
     1381 gacacagaca gaatcagcga ttctggcgca cgcccggcga tgtgcgccag cggagtcgtg
     1441 tggcttcgtg gtaagaacgc cggagggaga caggtatctt cccagcgaga atatctccgg
     1501 tgagccggag gaacggttcc ggatggctcc ggaggactgg ctgcgggcac aaatgcaggg
     1561 tgagattgtg gcgctggtcc acagtcatcc cggtggtctg ccctggctga gtgaggctga
     1621 ccggcggctg caggtgcaga gtgatttgcc gtggtggctg gtctgccggg gggcgattca
     1681 caagttccgc tgtgtgccgc atctcaccgg gcggcgcttt gagcacgggg tgacggactg
     1741 ttacacgctg ttccgggatg cttatcatct ggcagggatt gagatgccgg attttcatcg
     1801 cggggatgac tggtggcgta acggtcagaa tctctatctt gacaatatgg aggtgacggg
     1861 tttttaccgt gtcgcactga cagaggcaca gccgggcgat gtgctgctgt gctgttttgg
     1921 ttcatcggtg ccgaatcatg ccgccattta ctgcggtgac ggcgggctgc tgcaccatat
     1981 tcctgaacaa ctgagcaaac gagagaggta caccgacaaa tggcagcgac gcacacactc
     2041 cctctggcgt caccgggcat ggcgcgcatc tgcctttacg gggatttaca acgatttggt
     2101 cgccgcatcg accttcgtgt gaaaacgggg gctgaagcca tccgggcact ggccacacag
     2161 ctcccggcgt ttcgtcagaa actgagtgac ggctggtatc aggtgcgcat tgccgggcgt
     2221 gatgcaggtg aaaccgaatt gtctgcccgt cttaatgagc cgctggcaaa tggtgcagtg
     2281 atccacatag taccgcgtct ggtgggagct aaaagtggcg gtgtgtttca ggcggtgctg
     2341 ggggcggctg ttatggcggt tgctatatgg atgccggggg taggaattat ggcgagtaat
     2401 ctgctgtttt ctctcggtgc cagtatgacg cttggcggtg ttgcacagat gctggcaccg
     2461 aaagccagaa ctccccgtac acagacaacg gataacggta agcagaacac gtatttctcg
     2521 tcactggata acatggttgc ccagggcaat gttctgcccg ttctgtacgg tgaaatgcgc
     2581 gtggggtcac gtgtggtttc tcaggagatc agcacggcag acgaagggga tggtggtcag
     2641 gttgtggtga ttggtcgctg atgcaaaatg ttttatgtga aaccgcctgc gggcggtttt
     2701 gtcgtttatg gagcgtgagg aatgggtaaa ggcagcagta aggggcatac cccgcgcgaa
     2761 gcgaaggaca acctgaaatc cacgcagttg ctgagtgtga ttgatgccat cagcgaaggg
     2821 ccggttgacg gtccggtgga tggattaaaa agcgtgctgc tgaacggtac gccggtcctg
     2881 gacagcgagg ggaagacaaa cctttccggt gttacggtgg tgttccgtgc aggtgagcag
     2941 gagcagacac cgccggaggg ttttgaatcc tccggctccg agacggtgct gggtacggaa
     3001 gtgaaatatg acacgccgat cacccggacc atcacgtcgg caaacatcga ccgtctgcgc
     3061 ctgaccttcg gtgtgcaggc actggtggaa accacctcaa agggtgaccg gaatccgtcg
     3121 gaagtccgcc tgctggttca gatacagcgt aacggtggct gggtgacgga aaaagacatc
     3181 accattaagg gcaaaaccac ttcgcagtat ctggcctcgg tggtggtggg taacctgccg
     3241 ccgcgcccgt ttaatatccg gatgcgcagg atgacaccgg acagcaccac agaccagctg
     3301 cagaacaaaa cgctctggtc gtcgtacacc gaaatcatcg atgtgaaaca gtgctacccg
     3361 aacacggcgc tggtcggcgt gcaggtggat tcagagcagt tcggtaacca gcaggtgagt
     3421 cgcaattatc atcttcgcgg gcgcattctg caggtgccgt cgaactataa cccgcagacg
     3481 cggcaataca gcggtatctg ggacggaacg cttaagccag catacagcaa caacatggcc
     3541 tggtgtctgt gggacatgct cactcatccg cgctacggca tggggaaacg tcttggtgcg
     3601 gcagatgtgg acaaatgggc gctgtatgtc atcggccagt actgcgacca gtcagtgacg
     3661 gacggatttg gcggcacgga gccgcgcatc acctgtaact cttacctgac cacacagcgt
     3721 aaggcgtggg atgttctcag tgatttctgc tcggcgatgc gctgtatgcc ggtatggaac
     3781 ggacagatgc tgacgttcgt gcaggaccga ccgtcggata aggtgtggac ctataaccgc
     3841 agtaatgtgg tgatgccgga cgatggcgcg ccgttccgct acagcttcag cgccctgaag
     3901 gaccgccata atgccgttga ggtgaactgg attgacccag ataacggctg ggagacggcg
     3961 acagagcttg tggaggatac gcaggccatt gcccgttacg gtcgtaatgt cacgaagatg
     4021 gatgcctttg gctgtaccag ccgggggcag gcacaccgag ccgggctgtg gctgattaaa
     4081 acggaactgc tggaaacgca gaccgtggac ttcagcgtgg gcgcagaagg gcttcgccat
     4141 gtaccgggtg atgtcattga aatctgcgat gatgattatg ccggtatcag caccggtggt
     4201 cgcgtactgg cggtgaacag ccagacccgg acgctgacgc tcgaccgtga aatcacgctg
     4261 ccgtcctccg gtaccacgct gataagcctg gttgacggaa gtggcaatcc ggtcagcgtg
     4321 gaggtccagt ccgtcaccga cggcgtgaag gtgaaagtga gccgggttcc tgacggcgtt
     4381 gcagaataca gcgtgtgggg gctgaagctg ccgacgctgc gccagcgtct gttccgctgc
     4441 gtgagtatcc gtgagaacga cgacggcacg tatgccatca ccgccgtgca gcatgtgccg
     4501 gaaaaagagg ccatcgtgga taacggggca cactttgacg gtgaccggcg cggcacggtg
     4561 aatggtgtca cgccgccagc ggtgcagcat ctgaccgccg aagtcaccgc agacagcggg
     4621 gaatatcagg tgctggcgcg atgggacacg ccgaaggtgg tgaagggtgt gagcttcctg
     4681 cttcgcctga ccgtggcagc ggacgacggc agtgagcggc tggtcagcac agccaggacg
     4741 acggaaacca cataccgctt caggcagctg gcgctgggga attacagtct gacagtccgg
     4801 gcggtaaatg cccgggggca gcagggcgat ccggcgtcgg tatcgttccg gattgcggca
     4861 ccggcagcgc ctgtcactat tgaactgata ccggggtatt ttcagataac ggcggtcccg
     4921 aaactggctg tatatgaccc gacggtgcag tttgagttct ggttttcgga aaagcggatt
     4981 gcggatatca ggcaggttga aaccagcgcc cgctatcttg gcacggcgct gtactggata
     5041 gccgccagta tcaatatcag gccgggccat gattattatt tttacgttcg cagtgtgaac
     5101 accgttggca aatcggcatt tgtggaggcc atcggtcggg cgagcgatga tgcagaaggt
     5161 tatctgaatt tttataaagg gttgatcaat aaaacgcatc tcggcaagga gttgtggacg
     5221 cagattgata acggtcagct tgcgccggac ctgactgaaa tcaggacgtc cattacgaat
     5281 gtcagcaatg aaatcacgca aaccgtcaat aaaaaactgg aaaatcagag tgctgcaatc
     5341 cagcagatac agaaagttca ggttgataca aataataacc tgaacagcat gtgggctgtg
     5401 aagctgcaac agatgaagga cggacgcctt tatattgcgg gtatcggtgc cggtattgag
     5461 aatacgccag caggaatgca gagtcaggtg ctgctggcgg cagacaggat tgcgatgatt
     5521 aatcctgcga atggcaacac aaagccgatg tttgttggtc agggcgatca gatattcatg
     5581 aacgacgtgt tcctgaaatg cctgacggct ccgaccatta ccagcggcgg taatcctccg
     5641 gcattttccc tgacacctgg cggacggctg acggcgaaaa atgccgatat cagcggtaac
     5701 gtgaacgcga actccgggac gctcaacaac gtcacgatta ataagaactg tcgggttctg
     5761 ggaaaattgt ccgcgaacca gattgaaggc gatctcgtta aaacagtggg caaacctttc
     5821 ccacgggact cccgggcacc ggagaggtgg ccatcaggga ccattaccgt cagggtttat
     5881 gacgatcagc cgtttgatcg gcaaattgtt attcccgcgg tggcgtttcg cggtgctaaa
     5941 catgagcgga agaataacaa tatttattcg tcatgccgcc tgatagtgaa gaaaaacggt
     6001 gctgaaattt ataaccgaac gaccctggat aatacgctga tatatacggg tgttattgat
     6061 atgcctgccg gtcacggtca catgacgctg gagttttctg tatcggcatg gctggtaaat
     6121 ggctggtatc ccacagcaag tatcagcgat ttgctggttg tggtgatgaa gaaagccact
     6181 gcaggcatca cgattagctg aattttataa cccagatacg ggcaccagaa atggtgcctt
     6241 ttttattgca gaaaagcgag aggtaattat gcgtaaagtt tgtgcagcca ttttgtccgc
     6301 agccatctgt ctggccgtat ccggtgcgcc tgcatgggcg tctgaacatc agtccacgct
     6361 gagcgccggg tatcttcaga cccatactga tatgccaggc agcgatgacc tgaagggcat
     6421 taacgtgaaa taccgttatg agtttacgga cacgctgggg ctgattacgt cattcagtta
     6481 tgccaacgct gaagatgagc aaaaaacgca ttacagcgat acccgctggc atgaggattc
     6541 cgtgcgtaac cgctggttca gcgtgatggc ggggccgtct gtgcgcgtga atgaatggtt
     6601 cagcgcgtat gcgatggcgg gcgtggctta cagccgtgtg tcgaccttct ccggggatta
     6661 tctccgcgta actgacaaca aggggaaaac gcacgatgtg ctgaccggaa gtgatggcgg
     6721 tcgccacagc aacacgtctc tggcgtgggg ggctggcgtg cagtttaacc cgaccgaatc
     6781 cgtggccatt gaccttgctt atgaaggttc cggcagtggt gactggcgca ctgacggttt
     6841 catcgtgggt gtcggttata agttctgatt agccaggtaa cacagtgtta tgactgcccg
     6901 ctggttcagg cgggcttttt gtggggtgaa tatggcagta aagatttcag gtgtactgaa
     6961 agatggggcg ggtaaacctg tcgtaaattg tgcgattgaa ctgcgggcca gaagaaccag
     7021 tccgaccgtt gtggcacacg ttgttgccac ttgcgtgacg gacaataacg gtgcttatgt
     7081 gattgaggct gagccggggt attacgaggt tgcgcttcac tgtaacggct ggcagccaac
     7141 ccgtgtcggg gatattgatg tggcaccgac tgatgcaccg gggacactga acgcgtttct
     7201 gaatgcacca aaggatggtg atttacgtcc ggaggtgat
//
LOCUS       DAEEKQ010000189         7220 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.191, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000189 DAEEKQ010000000
VERSION     DAEEKQ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7220
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.191"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            230..1663
                     /gene="cscA"
                     /locus_tag="KLK57_003662"
     CDS             230..1663
                     /gene="cscA"
                     /locus_tag="KLK57_003662"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HBE0893930.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDCAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1671..2729)
                     /locus_tag="KLK57_003663"
     CDS             complement(1671..2729)
                     /locus_tag="KLK57_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0893931.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2897..3115
                     /locus_tag="KLK57_003664"
                     /pseudo
     CDS             2897..3115
                     /locus_tag="KLK57_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3133..4461
                     /gene="dsdA"
                     /locus_tag="KLK57_003665"
     CDS             3133..4461
                     /gene="dsdA"
                     /locus_tag="KLK57_003665"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HBE0893932.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="KLK57_003666"
     CDS             complement(4569..6107)
                     /gene="emrY"
                     /locus_tag="KLK57_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HBE0893933.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRLLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6107..>7220)
                     /gene="emrK"
                     /locus_tag="KLK57_003667"
     CDS             complement(6107..>7220)
                     /gene="emrK"
                     /locus_tag="KLK57_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HBE0893934.1"
                     /translation="SLLVIVLFIAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQV
                     SGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYS
                     AEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKAN
                     KALVMNTPLNRQPQVVEAADATKEAWLALKRTDIRSPVTGYIAQRSVQVGETVSPGQS
                     LMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAF
                     SLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKDEDIAEMPEL
                     ASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         1992 a         1722 c         1668 g         1838 t
ORIGIN      
        1 actttggctg acattttttt cccttaaatt catctgagtt acgcatagtg ataaacctct
       61 ttctcgcaaa atcgtcatgg atttactaaa acatgcatat tcgatcacaa aacgtcatag
      121 ttaacgttaa catttgtgat attcatcgca tttatgaaag taagggacta tatttttata
      181 aaagttaacg ttaacaattc accaaatttg cttaaccagg atgattaaaa tgacgcaatc
      241 tcgattgcat gcggcgcaaa acgcactagc aaaacttcat gagcaccggg gtaacacttt
      301 ctatccccat tttcacctcg cgccacctgc cggatggatg aacgatccaa acggcctgat
      361 ctggtttaac gatcgttatc acgcgtttta tcaacatcac ccgatgagcg aacactgggg
      421 accaatgcac tggggacatg ccaccagcga cgatatgatc cactggcagc atgagcctat
      481 tgcgctagcg ccaggagacg ataatgacaa agacgggtgt ttttcaggta gtgctgtcga
      541 tgacaatggt gtcctctcac ttatctacac cggacacgtc tggctcgatt gtgcaggtaa
      601 tgacgatgca attcgcgaag tacaatgtct ggctaccagt cgggatggta ttcatttcga
      661 gaaacagggt gtgatcctca ctccaccaga aggaatcatg cacttccgcg atcctaaagt
      721 gtggcgtgaa gccgacacat ggtggatggt agtcggggcg aaagatccag gcaacacggg
      781 gcagatcctg ctttatcgcg gcagttcatt gcgtgaatgg accttcgatc gcgtactggc
      841 ccacgctgat gcgggtgaaa gctatatgtg ggaatgtccg gactttttca gccttggcga
      901 tcagcattat ctgatgtttt ccccgcaggg aatgaatgcc gagggataca gttaccgaaa
      961 tcgctttcaa agtggcgtaa tacccggaat gtggtcgcca ggacgacttt ttgcacaatc
     1021 cgggcatttt actgaacttg ataacgggca tgacttttat gcaccacaaa gctttttagc
     1081 gaaggatggt cggcgtattg ttatcggctg gatggatatg tgggaatcgc caatgccctc
     1141 aaaacgtgaa ggatgggcag gctgcatgac gctggcgcgc gagctatcag agagcaatgg
     1201 caaacttcta caacgcccgg ttcacgaagc tgagtcgtta cgccagcagc atcaatctgt
     1261 ctctccccgc acaatcagca ataaatatgt tttgcaggaa aacgcgcaag cagttgagat
     1321 tcagttgcag tgggcgctga agaacagtga tgccgaacat tacggattac agctcggcac
     1381 tggaatgcgg ctgtatattg ataaccaatc tgagcgactt gttttgtggc ggtattaccc
     1441 acacgagaat ttagacggct accgtagtat tcccctcccg cagcgtgaca cgctcgccct
     1501 aaggatattt atcgatacat catccgtgga agtatttatt aacgacgggg aagcggtgat
     1561 gagtagtcga atctatccgc agccagaaga acgggaactg tcgctttatg cctcccacgg
     1621 agtggctgtg ctgcaacatg gagcactctg gctactgggt taacataata tcaggtggaa
     1681 caacggatca acagcggaca agggatccgc gtcactcttc ccccttcacg accttcaata
     1741 atatgcaatg cagcttcccg cccgataatg tcatgtggaa gctgaattgt ggtcagcggc
     1801 ggtaaaaaca gatgcccgac gccaaccaga ttatcaaagc ccattacggc gacatcctgc
     1861 gggatacgta cccccttcgc caggagaacc tgataagcga caaaggctgc acgatcgtta
     1921 ccacatatca gaacatcaaa atctggtttg cccgatttga agtgggcatt gagtaaactt
     1981 gcgagatcgg tgtagtgatc atcacctgtt gccatgtgaa attgtttcac ctcagccaga
     2041 tctcgtccag catcacgcca ggcctgctca aatccctgcc gacgataccc tgttgccaac
     2101 gcactttccg gtagccagaa gcataacggt tgacgatagc ccgccgcgag caaatgctgt
     2161 gttgattcat attgtgcagt gtaatcatca gggatataac tgggtaacgc tgggtcatcc
     2221 gccacacagt tcgccaatac aatattttca ccatacagag actcaggcag cgtgatatgt
     2281 cgcagcccca ttgtagtata gataatgcca tccggacggt gggcaagcag ctgacgtgcc
     2341 gcgcgggcag cgtcatcttc agaaaaaata ttgattaaaa aactattcca gccgaactcg
     2401 ctggcggttt gctcaatggc aagcagaata tcaacagaga aaggagtggt agccgtgtcc
     2461 tgcgccagca cggcgagagt cgacggctta cgtccttgag cgcgcatctt acgggcggaa
     2521 agatcaggaa cataattcag ggtctggatt gcctgcaata cgcggtcacg cgttgcagga
     2581 cgcacagatt ctgcattatg catcacccgg gagactgtca tcatcgacac tcccgccagg
     2641 cgtgcgacat cctttaatga agccataccc aagccgtttg ccgtaaaacg ggcactgtag
     2701 cagaaacaga cgtcactggc gagatccaac gccctatcac ctgacacagc aatacaataa
     2761 aaaataacaa taattcccgg acaattgtcc ccaattccgc ctctgttctc gccaacgagt
     2821 aaccatatag gcaagtaagt aaaacatgcc aactggcatc gccaacctgg agaagaaaat
     2881 ataaatatgg atcattgtgc tgccgctgta tcccgacatc agcccggaaa ttattgcgat
     2941 tgctatcggt tcaggtgcaa ttggctgcac gatcgttacg gactcgcttt tctggctagt
     3001 gaagcaatat tgcggcgcta cgctcaatga aacatttaaa tactatacga cagcgacatt
     3061 tatcgcttca gtcgtcgctc tggcgggcac attcctgctg tcatttatca tctaagcgca
     3121 aagagacgta ctatggaaaa cgctaaaatg aactcgctca tcgcccagta tccgttggta
     3181 aaggatctgg ttgctcttaa agaaaccacc tggtttaatc ctggcacgac ctcattggct
     3241 gaaggtttac cttatgttgg cctgaccgaa caggatgttc aggacgccca tgcgcgctta
     3301 tcccgttttg caccctatct ggcaaaagca tttgctgaaa ctgctgccac tggggggatt
     3361 attgaatcag aactggttgc cattccagct atgcaaaaac ggctggaaaa agaatatcag
     3421 caaccgatca gcgggcaact gttactgaaa aaagatagcc atttgcccat ttccggctcc
     3481 ataaaagcac gcggcgggat ttatgaagtc ctggcacacg cagaaaaact ggctctggaa
     3541 gcggggttgc tgacgcttga tgatgactac agcaaactgc tttctccgga gtttaaacag
     3601 ttctttagcc aatacagcat tgctgtgggc tcaaccggaa atctggggtt atcaatcggc
     3661 attatgagcg cccgcattgg ctttaaggtg acagttcata tgtctgctga tgcccgggca
     3721 tggaaaaaag cgaaactgcg cagccatggc gttacggtcg tggaatatga gcaagattat
     3781 ggtgttgccg tcgaggaagg acgtaaagca gcgcagtctg acccgaactg tttctttatt
     3841 gatgacgaaa attcccgcac gttgttcctt gggtattccg tcgctggcca gcgtcttaaa
     3901 gcgcaatttg cccagcaagg ccgtatcgtc gatgctgata accctctgtt tgtctatctg
     3961 ccgtgtggtg ttggcggtgg tcctggtggc gtcgcattcg ggcttaaact ggcgtttggc
     4021 gatcatgttc actgcttttt tgccgaacca acgcactccc cttgtatgtt gttaggcgtc
     4081 catacaggat tacacgatca gatttctgtt caggatattg gtatcgacaa ccttaccgca
     4141 gcggatggcc ttgcagttgg tcgcgcatca ggctttgtcg ggcgggcaat ggagcgtctg
     4201 ctggatggct tctataccct tagcgatcaa accatgtatg acatgcttgg ctggctggcg
     4261 caggaagaag gtattcgtct tgaaccttcg gcactggcgg gtatggccgg acctcagcgc
     4321 gtatgtgcat cagtaagtta ccaacagatg cacggtttca gcgcagaaca actgcgtaat
     4381 accactcatc tggtgtgggc gacgggaggt ggaatggtgc cggaagaaga gatgaatcaa
     4441 tatctggcaa aaggccgtta ataacgtttc aacgcagcat cgcaatcctt tccctgggtg
     4501 agcgatgctg ccgatggcgc agacttaaga tccccggtct tacccgctat aaccccctcc
     4561 ttttatcatc acccaacgcc tttcgctgta aacggcggtt tcgcaaacca aaccaaaacc
     4621 gtaagtaaga taaaagcaat agccgccata cggaaaatct catttgcaga aatagaaagt
     4681 gactgctggg ttatttcatt attaatttca ttaagaactc ctgaaagcga accataatat
     4741 ttatccataa tttgtgacga tgaattaaac acggggttaa attgatcgat ggttgctgtc
     4801 aactgactat ggtgtaacga ttcgcgtcgt ccccacagcg tcattgtcaa cgacgtacca
     4861 actgatcctg acaaggtacg aaaaaaatta ctcatactcg aggcattggc aaatttatta
     4921 tctggcaagc ctgaaaacga aatcgttgtt aagggtaaaa agaaacaggc aacggcgaat
     4981 ccctgaaaaa actgcggcaa aatgatgcct gtaaaatcaa tcgttggcat aaatgtcaca
     5041 gaacgccagt aatagcaaac cgcatacatc aaaaaactaa atgtcactaa caaccgcatg
     5101 tctattttgt tgccataacg tcctatcaaa ggtgaaatta atagtggcat gatgccgatg
     5161 ggcgcataag caagtccggc ccatatcgca ttatacccca tcgtttcctg gagtaactgc
     5221 ggcataagga cgatcgctcc agagtaaaat aaatacgcgc atgtgatact cacaatacca
     5281 atggtgaagt tacgggactt aaacaaactg agatcaagaa tcgggttctc tgaggtcgac
     5341 tcccaaatga ctaaagagat cagagaaata actgatacta ctgttaatat tattattgta
     5401 ctcgagttga accaatccag atcgcgccct ttatcaagca taatttgcaa gccaccaaca
     5461 ccgagcacta acagggtcag tcctggtaga ttcattttga ccggtgaagt ctcagtttct
     5521 cttcctttaa gtaaggttaa gcataatgtc aggacgataa tccccatagg gacattgatt
     5581 aaaaatatcc aaccccagct aaagttatca caaatataac cgcccaatat cggcccacat
     5641 atcggagcga taatcacggt cattgaccat aatgccagag caaatgttct tttttctggc
     5701 ggataattcc ttaataataa actctgtgac agtggaatta acggccccgc cattaacccc
     5761 tgaacgactc taaaaaatat cagcacatca agattggtcg ataggctaca cattaatgaa
     5821 gacagcgaaa aaaaggttac tgaaagtaaa aataatctta attcgcctat tctttgtgcc
     5881 aacctgccag taacagggat cgcaatggca tttgctacac caaacgaggt gataacccag
     5941 gtgccttcgt ctgttgatgc tcccagaaag ccagatattg tcggtattgc gacgttagaa
     6001 atagtggaat ccaacatttg cataaatgtc gctaatgaca atgcaatagt gacgcaccat
     6061 aacgtcccac cggttaatgg tgccggagtt gatttagtga ttgccattaa agttgtccat
     6121 tatgcgaaat aatgttgcta atttcttttt ctatcggact ggtatcgata actaaagcct
     6181 tactggtata agccggcatg gaggtcacgg ttgaagccag ctcaggcatc tcagcaatgt
     6241 cttcgtcctt cgtatcaata gttgcagtca tcgataaacc aatgcgcaag gggtgttcca
     6301 tgagttcttt tggatcaaga gaaacttcaa ccggtacacg ctgaacgatt ttgatccagt
     6361 tccctgtcgc attttgtgca ggtaataagg agaacgcatt gccggttccc atattgatcc
     6421 ctgtcacccg accatgaaac acaacatttt caccataaag atcgctgata atattgactg
     6481 attgaccaat ccgtacatcc gtgagttgtg tttctttaaa gttggcatta acccacattt
     6541 gacgtgccgg tacgacagcc attaacgatt gtccgggact cactgtttcg ccgacctgaa
     6601 cacttcgctg ggcaatatag ccagtaaccg gactccttat atccgtacgt ttaagcgcca
     6661 accaggcttc tttggttgca tccgccgctt caactacttg tggctgacgg tttaatggtg
     6721 tgttcattac taaagcttta ttcgctttat aagcctggat agcggcattc agtgccgctt
     6781 tgctacttat taacgtatct ttggtatgct ccagcgtttc ttttgaaata accccctgct
     6841 tcgctaaagg cacacgacgg ttataatctt ctaaggattg ttgatactga atacgtgctg
     6901 aagcgacttc ggcactgtat tgtttatcct gtaagtacag tttattcgtt tgccgaacaa
     6961 tatttgccag attattttta gctttattga gtgcgatagt ggcatcagtt ttatccagtg
     7021 aaactaaaat gtcaccctgt cgaacgtagt tcgtatcttt atgattaact acagtgacac
     7081 tacctgagac ttgtgcagaa attggatctg cattccccgt gacataggcg tcatctgtac
     7141 taatcatgtc ttctaattcc attgaccaat aggcataggc acctgaaaac gcaataaata
     7201 aaactatcac caataaagaa
//
LOCUS       DAEEKQ010000190         7121 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.192, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000190 DAEEKQ010000000
VERSION     DAEEKQ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.192"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            4..435
                     /locus_tag="KLK57_003668"
     CDS             4..435
                     /locus_tag="KLK57_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HBE0893935.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVDIVRLDAFKTSKEIQFA
                     VQSGPMLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            518..1420
                     /locus_tag="KLK57_003669"
     CDS             518..1420
                     /locus_tag="KLK57_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HBE0893936.1"
                     /translation="MKKTLATLFLLTCLGSSSAYADNALILQTDFSLKDGAVSAMKGV
                     AFGVDHNLKIFDLTHEIPPYNIWEGAYRLYQTASYWPQGSVFVSVVDPGVGTDRKSVV
                     LKTKNGQYFVSPDNGTLTLVAESLGIESVRQIDEKTNRLKGSEKSYTFHGRDVYAYTG
                     ARLASGAITFEQVGPELPAKVVELSYQKAKATKGEVKGNIPILDIQYGNVWSNISDEL
                     LNQAGIKLNDTLCVTISEGSQQKYAGKMPYVASFGDVPEGQPMVYLNSLLNVSVALNM
                     DNFAQKHQVASGADWNIDVKKCAK"
     gene            complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="KLK57_003670"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(1475..3637)
                     /gene="uvrD"
                     /locus_tag="KLK57_003670"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HBE0893937.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLETV"
     gene            complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="KLK57_003671"
     CDS             complement(3721..4437)
                     /gene="yigB"
                     /locus_tag="KLK57_003671"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HBE0893938.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="KLK57_003672"
     CDS             complement(4437..5333)
                     /gene="xerC"
                     /locus_tag="KLK57_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HBE0893939.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHLHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(5330..6037)
                     /locus_tag="KLK57_003673"
     CDS             complement(5330..6037)
                     /locus_tag="KLK57_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HBE0893940.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="KLK57_003674"
     CDS             complement(6034..6858)
                     /gene="dapF"
                     /locus_tag="KLK57_003674"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HBE0893941.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(6895..7098)
                     /locus_tag="KLK57_003675"
     CDS             complement(6895..7098)
                     /locus_tag="KLK57_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0893942.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
BASE COUNT         1640 a         1990 c         1854 g         1637 t
ORIGIN      
        1 aaggtggcgt taaatctcgc ttcaggtgaa gggaatttct ttatccgtcc tggcggcgtg
       61 ttttatgtcg cgggagataa agtcgacatc gttcgtctgg atgccttcaa aaccagtaaa
      121 gagattcagt ttgcggtgca gtcagggcca atgttgatgg aaaacagtgt aattaatccg
      181 cgtattcatc ccaacgtcgc ctcacgtaaa attcgtaacg gtgttgggat taataaacat
      241 gggaatgccg tgtttttgtt gagccagcag gcgacaaatt tttatgattt tgcctgttat
      301 gccaaagcga aactgaacgt tgagcagctg ctttatcttg atggcacgat ttcacatatg
      361 tatatgaaag gcggggcaat cccgtggcaa cgctatcctt ttgtcaccat gatttccgtc
      421 gagaggaaag gttaagcatt gtacactcga ggaggccaga caaactgggg ctggctacac
      481 agctgaaaaa tcatcagata aatgaaggga aacaaaaatg aaaaaaacgc tcgcaacgtt
      541 atttttatta acgtgtctgg gatcatcatc cgcttatgcg gataacgctt tgattttaca
      601 aactgatttt agcctgaaag atggtgccgt ctcggcgatg aaaggggtcg cattcggtgt
      661 tgaccataat cttaaaatat ttgatttaac acacgaaatt ccaccgtata acatctggga
      721 aggcgcttac cgcctgtatc agaccgccag ttactggccg caaggttcgg tatttgtcag
      781 tgtggtcgat cctggtgttg gtacagaccg taaatcggta gtgctaaaaa ccaaaaacgg
      841 tcaatacttc gtttcaccag ataacggtac gctcactttg gtggcggaat cgctgggtat
      901 tgagagcgtc cggcaaatcg atgaaaaaac caaccgcctg aaaggttccg aaaagtcata
      961 taccttccac ggtcgtgacg tgtatgccta caccggtgca cgcctggctt ccggagcgat
     1021 cacgttcgaa caggtggggc cagagcttcc tgcaaaagtt gtcgaacttt cttatcagaa
     1081 agccaaagca acaaaaggtg aagtgaaggg caatatcccg attctcgata ttcagtacgg
     1141 caacgtctgg agcaatatca gcgacgagtt gctgaatcag gcaggaatca aactcaacga
     1201 cacgttgtgt gtaaccattt ccgaaggttc ccagcaaaaa tacgcaggga aaatgccgta
     1261 tgtcgcaagc ttcggcgatg tgccggaagg ccagccgatg gtgtatctaa acagcttatt
     1321 gaatgtttcc gtggcgctca atatggataa tttcgcgcag aaacatcaag ttgcgtctgg
     1381 tgctgactgg aatattgatg tgaagaagtg cgctaagtaa ggtgctgatg caggatgcgg
     1441 tgtggcgcac cgcatcctgc aacggagttg taaattacac cgtctccagc cgggcgtaag
     1501 ccgccaccag ccatttgatt ccctggccct ggaatgccac ctgcaaacgg ctatgttcac
     1561 cgctgccttc catattgacg atggtgcctt caccaaactt agcgtggcgc acgcgctggc
     1621 cgagtttgta gccgctgtcg ttctcgacca tcggcgtacc catccgctga tggctaaccg
     1681 ggcggcttac cgtggcgcgc aggcgcacct cttccacaca ctcttccggc agctcgccga
     1741 taaagcgcga cgggcgatgg taaacctctt tgccatacag acggcgagtt tccgcgtagg
     1801 tcagcgtcag tttctgcatc gcacgggtta cgccaacgta ggccagacgg cgttcttctt
     1861 ccagacgtcc gccttcatcc agcgacatct ggcttgggaa catgccctct tccataccga
     1921 cgataaacac ctgcgggaac tccagccctt tcgccgagtg tagcgtcatc aactgcaccg
     1981 catcctgcca agtatccgcc tgcccttcac ctgcttccag tgccgcatgg gagaggaacg
     2041 cctgcagcgg cattaaatct tcgtcttctt cgttgtagct gaactggcgc gttgccgtca
     2101 ccagttcctc taagttttcg atacgcgtct gacctttttc gcccttctcc tgctcgtaca
     2161 tggtacgcag gccggagtct ttaattaccc ggtcagtctg tacatgcagc ggcatatcgg
     2221 cagtttcttg cgctaaggca tcgattaatt ccataaatcg ctgcaaggcg ctggcagctc
     2281 gcccggcgag ggctttttcc tgcaacagtt cacgacatgc ctgccagagt gttaactggc
     2341 gatcgcgcga tgtctgacgt accacgtcca gcgtccggtc accaataccc cgcgttggcg
     2401 tattcaccac gcgctcaaac gccgcgtcgt cgttacggtt ggcaatcaga cgcagatacg
     2461 agagcgcgtc tttgatttcc tggcgttcga agaagcgcat cccgccgtaa atacggtacg
     2521 gcatactggc ctgcaacagt gcttcttcca gtacacgcga ctgggcgtta ctgcggtaga
     2581 gaatggcgca ctcggcaagc gccccgccgt tgtcctgcca agttttgatg cggttaacca
     2641 caaaacgcgc ttcgtcgagt tcgttaaaag cgcaatagag ggaaataggc tcaccgtccg
     2701 cgccatcggt ccacagtttt ttacccagac gcccgttgtt gttttcaatc agggcgttcg
     2761 cggcgctcag aatattgctg gtagagcggt agttctgctc cagacggata gtttcagcac
     2821 cggggaaatc attgaggaaa cgctgaatat tctctacctg cgccccgcgc cagccgtaga
     2881 ttgactggtc gtcatcgcca acgatcatca ctttgccggt atcgcccgcc agcaggcgga
     2941 tccacgcgta ctgaatgttg ttggtatcct ggaattcgtc caccaggatg ttggtaaaac
     3001 gttcgcggta gtgttgcagg atatgcggct tgttaagcca caactcgtga gcgcgcagca
     3061 gcagctcggc gaagtccacc aggcccgcac gatcgcacgc ttcctgatac gcctgataca
     3121 ccttctgcca ggtctgctcc accggattac cgtagctttg aatatgatgc ggacgcaggc
     3181 cttcatcttt ctggctgttg atgtaccaca ttgcctggcg cggcggccac tgcttctcgt
     3241 cgaggttcat ggctttgatc agacgcttaa gcaggcgtag ctggtcttca ctgtcgagga
     3301 tctggaaatc ctgcggtaga ttggcgtcca tatggtgcgc acgcagcaaa cggtgcgcca
     3361 gcccgtggaa ggtgccgacc cacatgccgc cctggctggt gcccatcagt tgcccgatac
     3421 gatgacgcat ctccgccgcc gctttgttgg taaacgtcac agccataatc gagtatggcg
     3481 agcagttttc cacgctcatc agccaggcga tacgatgcac cagtacgcgc gtcttaccac
     3541 tgcccgcgcc cgccagcacc agaaggttgc tgcgtggcgc ggccaccgct tcgcgctgtt
     3601 tgtcattaag gctgtcgagc aggtaagaaa cgtccattgg caccgccgcg taaaaatagg
     3661 ttgggcggag aagcgcaacg ccctccgcca aaaagctggg tatatataca gatttactga
     3721 ttatatcagc gaggtcagag atgccaaccg ggaaatttcc agatgcggca gtaaacggct
     3781 gtcccaggtt tgcatcagat cgccattttc cggtctgatc caacaagcct gcattccgct
     3841 gcgaattgcc ccacccacgt cagtggtgag atcgtcccca acatgtaaga tctcgccgat
     3901 cggcacgttg agtttttccg cagccaaaaa gtacatatcg ctgaacggtt ttgagcgccc
     3961 atgcgggcca gcgcgcagca caaactcaaa ataatccccc aaaccaaaca gttccggctg
     4021 ggcgttaccg ttggtgatcg ccaccagcgg ccatttcttc gccagctgtt ttaaggtgtc
     4081 gtgagtttgc tgcgggacgt cgattcggct gcgccatttg gcaaagttaa tcattgctgc
     4141 gtgtgcgcct gcactggctt cttcggcact cagcccggcg tcgagcatcg cttgttcaat
     4201 cgaacgaaaa cgccaacgcg tcacgtcgtg ataaatctcg ggttccgctt cccgtaccgc
     4261 ctggcgcagg cgttgcagat cttcattctg gaagctgcgc agcgccggat gataattttg
     4321 cacaaaggta agcgcctctc gctcggtgcg caaaatcacc ggacggttat cgtaaagggt
     4381 atcatccagg tcaaaggtga gcgccgagat gcgccccaaa ggccggtaaa aacgcattat
     4441 ttcccccgtt tggcgcgtgg atgcgccgca tcgtacaccg aggcaaggtg ttgaaaatca
     4501 agatgagtat agatttgcgt ggtggagagg ttggcatgac ccagcagctc ctgcacacca
     4561 cgaagatcgc cgctcgactc cagcatatgc gtggcgaacg agtgacgtaa tttatgcgga
     4621 tgaaggtgat tattcagccc ttgttttatg ccccattcgg caaagcgttt ctgcacatta
     4681 cgcgcggaga tacgcttgcc cagtttcgac agaaaaagcg cgtcgtcttc gctaccaaac
     4741 aggtcgcgca aatcaagcca gtgctcaatc cacgccacag cgttgcgacc aatcggcagg
     4801 cggcgctctt tgctgccttt ccccattacc cacacttcac cagactccag gtcgaggtgt
     4861 ttgatatcca gccccaccag ctcggaaaga cgcagacccg cgccgtacat cacttccagc
     4921 attgcacggt cgcgtacagc gaggggatcg ttgatatcaa tgtccagcag ccgattcata
     4981 tcgtcgacgt cgatattttt cggcagatga cgcggcgctt tcggtgccga aacacctttc
     5041 gccgggttag ctttgagttc gttctggctg accagccagt caaaaaagct acgtagcgca
     5101 gaaagccgta acgcgaggct tgctgctccc agccctttac ggcgactgcg tacagcaaaa
     5161 ttgcgcacca tcgtcacatc acattgctgc cagctttgca ggccgttttc gctggcaaaa
     5221 ttgatgatcg cctcaagctg acgctggtag ttaagcaggg ttatcgggct aagctggcgc
     5281 tccacgctca gataacgtag gtagcgttct acatcggtgt gtaaatcggt catacgcgtt
     5341 caatccaacg ctccagaagc tccggcaaca tcagcgcaat ttcatgaagt aactgcgttc
     5401 cctgcccttg ttgatagtga ctggcatcgc gactggtaaa cagcacgaca cccaaatcag
     5461 catcgcttcc cagcatcgac atcgccaccg atcccaccgc tttcgcttcc ggtagcacca
     5521 ccagcagctc tggtccgtta agcggcccaa gatagtgctg ttcctgcccc aaacgctgaa
     5581 tacgcagcgg ttcgaaagac tgacggctta atgccagatg agtgtggttc gacggcgcac
     5641 ctaagcgcca gcgatccgga aacaggcgca gactcgcacc tgccaggccg agatcgcgcg
     5701 cccagcggtg aaagcgcatc agcatatcgt cgagactgct ggcggcggtg agactgcgct
     5761 gcaggtagag taggcgataa aacaggcctt cgttggcgat agcctgttcc atcaacagcg
     5821 ccatgttctc ttccagaaca tgaatatgat tacgtgcgcg ggccatgtgc cactcgacca
     5881 acgaaacggt gccgcgtacc ggatgcggca cacgtatcgc ttctactgcg cgcgcattac
     5941 ggataaaaaa ctcaggattt ttaatcagat aatcgacaac cgcccggtca tcaagctccg
     6001 tgagtgtttc ctgcagttct tcccctggtt gcttcataga tgaataaatc cgtcgtagac
     6061 atgtaccgcc gggccagtca tatataacgg gtgacccgga cctttccagg cgatatcaag
     6121 acgaccgccg gggagttcca cgcgtacttc ttcggccagc aaaccttgct gaatccctac
     6181 tgcaaccgcc gcacacgcgc cgctgccgca ggcctgggtt tctcctgccc cacgctcata
     6241 aacgcgtaaa cgaatatgct cgcgcttaac cacttgcata aaaccgatat tggcgcgctc
     6301 cggaaaacgc tcgtggcttt ccagaacagg accaagcgtt tctaccgccg cggtatcgac
     6361 atcatcgacc tgaatcacgc aatgcggatt tcccatcgac accacgccgc ataagattgt
     6421 ctgctcggcg gcgcgcataa tataggtctt ttccgctttg ttagcgcgaa acggcacggc
     6481 ggaaggttcg aagttgggtt cgcccatatt tacgcggacc agatcatcat cggtgacggt
     6541 cagaaccatc cgcccgttgg cggtgctgac gcggatatca cgcttattgg tcagtccttt
     6601 cagacgcaca aaacgggcaa agcagcgcgc accgttgccg cactgcgcca cttcactgcc
     6661 atcagcattg aaaatgcgat agtgaaaatc cagttcagga tcatacggcg gctcaaccac
     6721 cagcagttgg tcaaacccta cccccaggtg ccgatcagcc aggcgacgaa tcagctccgg
     6781 tgaaaaaaag acattctgcg ttaccgcgtc gacgaccata aaatcgttgc caaggccatg
     6841 cattttcgag aactgcatca tttactccaa tcacgcgggt acagaaactg acttttagta
     6901 attcacctgg gatggaccat cgccagtggc gcgatcgttt ttatccggca ccgtggattg
     6961 cgtttgcgtc tctaccggtt tggtcggcgg cggtgcgttt ttatctgcag gcgggaaata
     7021 gagcggacct ttcagaccgc agcccgtcag gctgaagaga gtaagtaata cagtgagtgc
     7081 cttaaacacg tttttcatta tggattgcct gtaaagttca t
//
LOCUS       DAEEKQ010000191         7098 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.193, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000191 DAEEKQ010000000
VERSION     DAEEKQ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7098)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7098)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7098
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.193"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            573..2681
                     /locus_tag="KLK57_003676"
     CDS             573..2681
                     /locus_tag="KLK57_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBE0893943.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTVWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRI
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASDLHGEQPQAVPGR
                     RGSGTTLENHFAVIPADRTWRPQPLMKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHAQKNMNTEVLNN
                     RTTDVINNHAETIGNNQMIAVTNNQIETVGVNQIETVGSNQIIKVGSVQVETIGLVRA
                     LTVGVAYQTTVGGIMNTSVALMQSSQIGLHKSLRVGLGYDVKVGNNVTFTVGKTKKDD
                     TGQTAIYSAGEHLELCCGKARLVLTKDGQIFLNGTKIHLQGKEQVNGDSLLINWNCAA
                     SKSPPKTPDEKQDTPDMREY"
     gene            2749..6291
                     /locus_tag="KLK57_003677"
                     /pseudo
     CDS             2749..6291
                     /locus_tag="KLK57_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT         1697 a         1773 c         2201 g         1427 t
ORIGIN      
        1 gaagcaagat catccgggca ccaggtggtg gtgcagtgct ttggaaatgg tcctctcggg
       61 gggagttgcc attattttct atgctatttg cttattatat tcatcaggtg aatttaataa
      121 attcctaatt tgaaatatcc actattaagc cagtgtttaa cgacgttaag atggcaatgt
      181 gcagatcatg ttatcagggt gggcggtagc gtatattcct tatacaaacc atcattcatt
      241 tcaatgattg ttattgaatt atttattgtt gtgaataaaa agacattatg aatattggtt
      301 tgactttgca tatcaggttt tttattactc gctgtaatgt acgagcctgt cgtgaataac
      361 gtcgagaacc catccgttgc gtttgtgatt attttgttga ctaaacagac acccgtttct
      421 ctgaagtaaa atcccagact aaatcatcac ataaccatga catttttctg atattccccg
      481 gtaacgccag atgtcgactc gcttatctca tccgcccgac atgttgtaca tgcaatatgt
      541 gcggattatt attcattttc acggaggttg ctatgtcaac cggattacgt ttcacactgg
      601 aagtggacgg cctgccaccg gatgcttttg cggtggtttc ctttcatttg aaccagtcac
      661 tctcttcgct cttttccctc gatctctctc tggtcagcca gcagtttctc tcccttgaat
      721 ttgcgcaggt gctggacaaa atggcctacc tgacggtatg gcagggcgat gacgtacagc
      781 gccgggtgaa aggtgtggtg acctggtttg aactggggga gaacgacaaa aaccagatgc
      841 tgtacagcat gaaggtatgc ccaccgctgt ggcgcatagg gctgcgccag aacttccgta
      901 tcttccagaa tgaggacatc gaaagcatcc tcgcgacgat cctgaaggaa aacggtgtga
      961 ccgagtggag tccgctgttc agcgagccgc atccttcccg tgagttttgt gtccagtacg
     1021 gcgaaactga ttacgatttc ctgtgccgga tggcggcgga ggaaggcatc ttcttttatg
     1081 aggagcacgc gcaaaaaagt accgaccaga gcctggtcct gtgcgacacc gtgcgttatc
     1141 tgccggagtc ctttgagatc ccctggaacc cgaacacccg taccgaggta agtaccctct
     1201 gcatcagcca gtttcgctac agcgcacaaa tccgtccttc ttccgtagtg accaaagact
     1261 acacctttaa acgtcccggc tggccagggc gttttgatca ggaaggccag taccaggatt
     1321 accagcgcac gcagtatgaa gtgtatgact accccggacg tttcaagggt gcccacgggc
     1381 agaactttgc ccgctggcag atggacggct ggcgaaacaa cgcagaagtg gcgcgcggaa
     1441 caagccgttc gccggagata tggccgggac ggcgaattgt gctgacgggg catccgcagg
     1501 cgaacctgaa ccgggaatgg caggtggtgg cgagcgatct tcacggcgaa cagccgcagg
     1561 cggtaccggg acgcaggggt tcaggcacca cgctggagaa ccattttgcg gtaatcccgg
     1621 cagacagaac atggcggcca cagccgttga tgaaaccgct ggtggatggc ccgcagagcg
     1681 ccgtcgtgac gggaccggca ggcgaggaaa tcttctgtga tgaacatggc cgcgtgcggg
     1741 tgaaatttaa ctgggaccgc tataacccgt caaaccagga cagttcatgc tggatccgtg
     1801 tggcacaggc gtgggcaggc accggctttg gcaacctggc gataccgcgt gtgggtcagg
     1861 aggtgattgt ggacttcctc aacggcgacc cggaccagcc gattattatg gggcgtacct
     1921 accaccagga aaaccgcaca cccggcagcc tgccggggac gaagacgcag atgaccattc
     1981 gttcgaaaac ctataagggc agcgggttta atgaactgaa gtttgacgat gcgacaggga
     2041 aagaacaggt ctacatccac gcgcagaaga acatgaacac cgaggtgctg aataaccgca
     2101 ccacggatgt gataaacaac catgctgaaa ccattggcaa caatcagatg attgcggtta
     2161 ccaacaatca gatagagacg gtgggcgtta accagataga gacggtgggc agtaaccaga
     2221 taattaaagt gggttctgtt caggtggaaa cgattggact tgttcgtgcg ctgacggtgg
     2281 gcgtggcgta tcagacgacg gtaggtggca ttatgaacac ctcggtggca ctgatgcagt
     2341 cctcgcagat cggtttgcat aaatcgttga gggtcgggct gggttatgac gtcaaagtcg
     2401 gaaataacgt taccttcacc gttggtaaaa cgaaaaagga tgataccggg cagaccgcga
     2461 tttactccgc cggtgagcat ctggagctct gctgtggtaa ggcaaggctg gtgctgacga
     2521 aggacggaca aatttttctc aacggcacaa aaattcattt gcagggtaag gagcaggtta
     2581 atggtgactc actgttgatt aactggaact gtgcagcctc gaaatcccca ccgaagaccc
     2641 ctgatgaaaa gcaggatacg ccggatatga gagagtactg atcccgtacc tgttcggtat
     2701 tcagtaacaa accagcatta aaaataaaca gaagaaggaa tttgaatcat gagcggaaaa
     2761 ccagcggcgc gtcagggaga tatgactcag tatggcggtc ccattgtcca gggttcggca
     2821 ggtgtaagaa ttggcgcgcc caccggcgtg gcgtgctcgg tgtgtccggg cgggatgact
     2881 tcgggcaacc cggtaaatcc gctgctgggg gcgaaggtgc tgcccggcga gacggacctt
     2941 gcgctgcccg gcccgctgcc gttcattctc tcccgcacct acagcagcta ccggacgaag
     3001 acgcctgcac cggtgggcgt tttcggcccc ggctggaaag cgccttctga tatccgctta
     3061 cagctacgtg atgacggact gatactcaac gacaacggcg ggcggagcat tcactttgag
     3121 ccgctgctgc cgggggaggc ggtgtacagc cgcagtgagt caatgtggct ggtgcgcggt
     3181 ggtaaggcag cacagccgga cggccatacg ctggcgcggc tgtggggggc gctgcgccgg
     3241 atatccggtt aagcccgcat ctttacctgg cgaccaacag cgcacagggg ccgtggtgga
     3301 tactgggggg ggctgagcgg gtgccgggtg ctgaggacgt actgccagcg ccgctgccgc
     3361 cgtaccgggt gcttaccggg atggcggacg ctgacgtacc ggcgtgaggc cgccggtgac
     3421 ctggccgggg aaatcaccga cgtgacggac ggtgccgggc gggagttccg tctggtgctg
     3481 accacgcagg cgcagcgtgc ggaagaggcc cgcacctctt cgctatcttc ttctgacagt
     3541 tcccgccctc tctcagcctc agcgttcccc gacacactgc ccggtaccga atacggcccc
     3601 gacaggggta tccgcctttc ggcggtgtgg ttgatgcacg acccggcata cccggagagc
     3661 ctgcccgctg cgccactggt gcggtacacg tatacggaag ccggtgaact gctggcggta
     3721 tatgaccgca gcaatacgca ggtgcgcgct ttcacgtatg acgcgcagca cccgggccgg
     3781 atggtggcgc accgttacgc gggaaggccg gagatgcgct accgctacga cgatacgggg
     3841 cgggtggtgg agcaactgaa cccggcaggg ttaagctacc gctatcttta tgagcaggac
     3901 cgcatcaccg tcaccgacag cctgaaccgg cgtgaggtgc tgcatacaga aggcggggcc
     3961 gggctgaaac gggtggtgaa aaaagaactg gcggacggca gcgtcacgcg cagcgggtat
     4021 gacgcggcag gaaggctcac ggcgcagacg gacgcggcgg gacggcggac agagtacggt
     4081 ctgaatgtgg tgtccggcga tatcacggac atcaccacac cggacgggcg ggagacgaaa
     4141 ttttactata acgacgggaa ccagctgacg gcggtggtgt acccggacgg gctggagagc
     4201 cgccgggaat atgatgaacc gggcaggctg gtatcggaga catcgcgcag cggggagaca
     4261 gtacgctacc gctacgatga cgcgcacagt gagttaccgg cgacgacaac ggatgcgacg
     4321 ggcagcaccc ggcagatgac gtggagccgc tacgggcagt tgctggcgtt cactgactgc
     4381 tcgggctacc agacccgcta tgaatacgac cgcttcggcc agatgacggc ggttcaccgc
     4441 gaggaaggta tcagccttta ccgccactat gacaaccgtg gccggttaac ctcggtgaaa
     4501 gacgcacagg gccgtgaaac gcagtatgag tacaacgccg caggcgacct gactgccgtt
     4561 atcaccccgg acggcaaccg gagcgagaca cagtacgatg cgtggggaaa ggcggtcagc
     4621 accacgcagg gcgggctgac gcgcagtatg gagtacgatg ctgccggacg tgtcatcagt
     4681 ctgactaacg agaacggcag tcacagcgac ttcagctacg atgcgctgga ccggctggta
     4741 cagcagggcg gctttgacgg gcggacgcaa cgttatcatt atgacctgac cggaaaactc
     4801 acgcagagtg aggatgaggg gcttgtcacc ctctggcact acgatgcgtc ggaccgcatc
     4861 acgcaccgga cggtgaacgg cgacccggca gagcagtggc agtatgacgg ccacggctgg
     4921 ctgagagaaa tcagccacct gagcgaaggc caccgtgtcg ctgtccacta cggctacgac
     4981 gataagggcc gcctgacggg cgaacggcag acggtggaga acccggagac gggggaactg
     5041 ctgtggcagc atgagacgaa acacgcctac aacgaacagg ggctggcaaa ccgcgtcacg
     5101 ccggacagcc tgccgccggt ggagtggctg acctacggca gcggttacct ggcgggcatg
     5161 aagctgggcg ggacgccgct ggtcgagtat acgcgggaca ggctgcaccg tgagacggtg
     5221 cgcaccctcg caccggacct ggacatccgc atcccgtatg ccaccgaccc ggcgggaaac
     5281 cgcctgccgg acccggagct acacccggac agcacgctca cggcgtggcc ggataaccgc
     5341 atcgcagagg atgcgcacta tgtttaccac tatgatgaat acggcagact gacggagaag
     5401 acggaccgca tcccgacggg tgtgatacgg acggacgacg agcggaccca ccactacgac
     5461 agccagcacc gcctggtgtt ccacacgcgg atacagcatg gcgagccact ggtcgagagc
     5521 cgctacctct acgacccgct gggacggcga atggcgaaac gggtatggcg gcgggagcgt
     5581 gacctgacgg ggtggatgtc gctgtcgcgt aaaccggagg agacatggta cggctgggac
     5641 ggcgacaggc tgacgacggt acagaccgac accacacgta tccagacggt atacgagccg
     5701 ggaagcttca cgccgctcat ccgggtcgag acagagaacg gcgagcggga gaaagcgcag
     5761 cgccgcagcc tggcggagaa gctgcagcag gaagggagcg aggacgggca cggcgtggta
     5821 tttccggctg aactggtgcg gctgctggac agactggagg aagaaatccg ggcagaccgc
     5881 gtgagcagtg aaagccgggt gtggcttgcg cagtgcgggc tgacggtgga gcaactggcc
     5941 agacaggtgg agccggaata cacaccagtg cgaaacgttc atctttacca ctgcgaccat
     6001 cggggccagc cgctggcgct catcagcgaa gacggcgata cggtgtggag cggggcgtat
     6061 gatgagtggg gcaaccagct aaatgaggag aacccgcatc atgtgtatca gccataccgt
     6121 ctgccggggc agcagtatga tgaggagtcg gggctgtact acaatcgtca ccggtactat
     6181 gatccgttgc agagacggta catcacccag gaccccattg ggctgaaagg tgggtggaat
     6241 ctgtatcagt atccgctgaa tccggtgagc aggatagatc cgttggggtt attctcatgt
     6301 gagagttgta gtcattttat agatgatata aataataaac tggataacct tccgtttaac
     6361 ccgacaaaaa tgtttacagc tagtttaaat actgctaatt cagcaaggct gtatgcaagt
     6421 ggcatgacaa aattcggtat aagtgttgct gccgatggaa cgatcattgc tgccccagtc
     6481 ggtgcagctt ctatggcttt aggggcgtgg aatataaaat ctgcgatagc tgcatataac
     6541 agagcaaatt tacaattatc agaatcactt caggaaaatg gtagagatag gtgttggaga
     6601 aatttattgg gtattttacc ttatggtgag cagtttgatg accctggtga accatcaata
     6661 ttagacgttt atggtgaaaa gattgagaat atcaaagatt ctccaatgaa gttttttgaa
     6721 gagcttggga cgctggggtt gtaatgtgta tttttaatga tatttttatg tttgtgttta
     6781 ttgttagtag tatacttgta accgtgcatt ttgtgtattc tttatttttt aaaagtggaa
     6841 aggtaaaaga taaggtaaaa aaatggatta aagtaatgtg tgatttattt tgggggatag
     6901 gatagtggtg tgatatggat aaatataaat tacagtggct ggcatatttc gacctgacgc
     6961 cggagtagat aaaaaaacag acaggattgg agtatagcaa gcgtctgaca atctctcggg
     7021 acagtagtat gatgaagagg cggggttgta ctataaccgt aaccggtact acgatccgct
     7081 aaagggcaag tatatgac
//
LOCUS       DAEEKQ010000192         7023 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.194, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000192 DAEEKQ010000000
VERSION     DAEEKQ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.194"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            522..1511
                     /gene="araF"
                     /locus_tag="KLK57_003678"
     CDS             522..1511
                     /gene="araF"
                     /locus_tag="KLK57_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HBE0893944.1"
                     /translation="MHKFTKALAAIGLAVVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDALSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1581..3095
                     /gene="araG"
                     /locus_tag="KLK57_003679"
     CDS             1581..3095
                     /gene="araG"
                     /locus_tag="KLK57_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HBE0893945.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRSIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3110..4096
                     /gene="araH"
                     /locus_tag="KLK57_003680"
     CDS             3110..4096
                     /gene="araH"
                     /locus_tag="KLK57_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HBE0893946.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRIV"
     gene            4263..5063
                     /gene="otsB"
                     /locus_tag="KLK57_003681"
     CDS             4263..5063
                     /gene="otsB"
                     /locus_tag="KLK57_003681"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HBE0893947.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            5038..6462
                     /gene="otsA"
                     /locus_tag="KLK57_003682"
     CDS             5038..6462
                     /gene="otsA"
                     /locus_tag="KLK57_003682"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HBE0893948.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6469..6897)
                     /gene="uspC"
                     /locus_tag="KLK57_003683"
     CDS             complement(6469..6897)
                     /gene="uspC"
                     /locus_tag="KLK57_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HBE0893949.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKFIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1779 a         1629 c         1823 g         1792 t
ORIGIN      
        1 ttgaattcat gatgaagtga tgattttata aaacgttttt tcatttttgc gagatggctc
       61 tcattatacg tgttctgtta attaatgagc acagtgataa ttaatggcga aatgaatgac
      121 gtgcattttc cacatctttg agttgcggtt attacaccat ttcaaaaact caacgccagg
      181 taatgcggcc tattgactgg ttaaaaagaa gacatcccgc atgggtacca aagacaacaa
      241 ggatttccag gctaatctta tggattaatc tgctgtgcat tcgacaattt gtctgacaaa
      301 ttggctttcc cttatgtctt ttcccgctaa atttatgcac gttctcactg taattctgcg
      361 atgtgatatt gctctcctat ggagaattaa tttctcgcta aaactatgtc aacacggtca
      421 cttatctttt agataaaagg taatgctttg ttttccgatt aatttaacga aagttattcg
      481 tttttgccct acacaaaacg acactaaagc tggagagaac catgcacaaa tttactaaag
      541 ccctggcagc cattggtctg gcagtcgtta tgtcacaatc cgctatggcg gagaacctga
      601 agctcggttt tctggtgaag caaccggaag agccgtggtt ccagaccgaa tggaagtttg
      661 ccgataaagc cgggaaggat ttagggtttg aggttattaa gattgccgtg ccggatggcg
      721 aaaaaacatt gaacgcgatc gacagcctgg ctgccagtgg cgcaaaaggt ttcgttattt
      781 gtactccgga ccccaaactc ggctctgcca tcgtcgcgaa agcgcgtggc tacgatatga
      841 aagtcattgc cgtggatgac cagtttgtta acgccaaagg taagccaatg gataccgttc
      901 cgctggtgat gatggcggcg actaaaattg gcgaacgtca gggtcaggaa ctgtataaag
      961 agatgcagaa acgtggctgg gatgtcaaag aaagcgcggt gatggcgatt accgccaacg
     1021 aactggatac ggcacgtcgc cgtacaacgg gttctatgga tgcgctgaaa gcggccggat
     1081 tcccggaaaa acaaatttat caggtaccta ccaaatctaa cgacatcccg ggggcatttg
     1141 acgctgccaa ctcaatgctg gttcaacatc cggaagttaa acattggctg atcgtcggta
     1201 tgaacgacag caccgtgctg ggcggcgtac gcgcgacgga aggtcagggc tttaaagctg
     1261 ccgatatcat cggcattggc attaacggtg tggatgcgtt gagcgaactg tctaaagcac
     1321 aggcaaccgg cttctacggt tccctgctgc caagcccgga cgtacatggc tataaatcca
     1381 gcgaaatgct ttacaactgg gtagcaaaag acgttgaacc gccaaaattt accgaagtta
     1441 ccgacgtggt gctgatcacg cgtgacaact ttaaagaaga actggagaaa aaaggtttag
     1501 gcggtaagta atttgccgga aaaattcccc tctgcatgat gcagaggggg tgtgaacgac
     1561 cagtgattca cggagacgtt atgcaacagt ctaccccgta tctctcattt cgcggcatcg
     1621 gtaaaacgtt tcccggcgtt aaggcgctga cggatattag ttttgactgc tatgccggtc
     1681 aggttcatgc gttgatgggt gaaaatggcg caggaaaatc aactctctta aaaatcctca
     1741 gcggcaacta tgcgccaacc acgggttctg tagtgattaa tgggcaggaa atgtcctttt
     1801 ccgacacgac cgcagcactt aacgcgggtg tggcgattat ttaccaggaa ctgcatctcg
     1861 tgccggaaat gaccgtcgcg gaaaacatct atctcggcca gctgccgcat aaaggcggca
     1921 ttgtgaatcg ctcattgctg aattatgagg cgggtttaca acttaaacat cttggtatgg
     1981 atattgaccc ggacacgccg ctgaaatatc tctccattgg tcagtggcag atggttgaaa
     2041 tcgccaaggc gctggcgcgt aacgctaaaa ttatcgcctt tgatgagcca accagctccc
     2101 tttctgcccg cgaaatcgac aatcttttcc gcgttattcg tgaactgcga aaagaggggc
     2161 gggtgatctt atacgtttct caccgtatgg aagaaatatt tgccctcagc gatgccatca
     2221 ctgtctttaa agatggacgt tatgtcaaaa cctttaccga tatgcagcag gttgaccacg
     2281 acgcgctggt gcaggcgatg gtcgggcgcg acattggcga tatctacggc tggcaacctc
     2341 gtagttatgg cgaggagcgc ctgcgtcttg atgctgtgaa agcaccaggc gtgcgtacgc
     2401 caataagtct ggcggttcgc agtggtgaaa ttgttgggct gtttggtctg gtaggggcgg
     2461 ggcgtagcga attaatgaaa ggcttgtttg gcgggacgca aatcaccgcc ggtcaggttt
     2521 atatcgacca acagccgatc gatattcgta aaccgagcca cgccattgcc gcaggcatga
     2581 tgctctgccc ggaagatcgc aaagcggaag gcattattcc cgtgcactcc gttcgcgaca
     2641 atatcaacat cagtgccaga cgcaaacatg tgctcggcgg ttgtgtaatc aacaacggtt
     2701 gggaagaaaa caatgccgat cagcacattc gttcgctcaa catcaaaacg ccgggcgctg
     2761 agcaactgat catgaatctc tcaggcggaa atcagcaaaa agccattctg ggccgctggt
     2821 tatcggaaga gatgaaggtc attttgctgg atgaacctac gcgcagcatt gatgttggtg
     2881 ctaagcacga aatatataac gtaatttatg cgctggcggc gcagggtgtg gcggtgctgt
     2941 ttgcctccag cgacttacct gaagtcctcg gcgttgccga ccggattgtg gtgatgcggg
     3001 aaggtgaaat cgccggtgaa ttgttacacg agcaggcaga tgagcgtcag gcactgagcc
     3061 ttgcgatgcc taaagtcagc caggcagttg cctgagtaag gagagtatga tgtcttctgt
     3121 ttctacatcg ggttctggcg cacctaagtc gtcattcagc ttcgggcgta tctgggatca
     3181 gtacggcatg ctggtggtgt ttgcggtgct ctttatcgcc tgtgccattt ttgtcccaaa
     3241 ttttgccacc ttcattaata tgaaagggtt gggcctggca atttccatgt cggggatggt
     3301 ggcgtgtggc atgttgttct gccttgcttc cggtgacttt gacctttctg tcgcctccgt
     3361 aattgcctgt gcgggtgtca ccacggcggt ggttatcaac ctgactgaaa gcctgtggat
     3421 tggcgtggca gcggggttgc tgcttggcat tctctgtggc ctggtcaatg gctttgttat
     3481 cgccaaactg aaaataaatg ctctgatcac aacactggca acgatgcaga ttgttcgagg
     3541 tctggcgtac atcatttcag acggtaaagc ggtcggtatc gaagatgaaa gcttctttgc
     3601 ccttggttac gctaactggt tcggtctgcc tgcgccaatc tggctcaccg tcgcgtgtct
     3661 gattatcttt ggtttgttgc tgaataaaac cacctttggt cgtaacaccc tggcgattgg
     3721 cgggaacgaa gaggctgcgc gtctggcggg tgtaccggtt gttcgcacca aaattattat
     3781 ctttgttctc tctggcctgg tatctgcgat agccggaatt attctggctt cacgtatgac
     3841 tagtgggcag ccaatgacgt cgattggtta tgagcttatt gttatctccg cctgcgtttt
     3901 aggtggcgtt tctctgaaag gtggcatcgg aaaaatctca tatgtggtgg cgggtatctt
     3961 aattttaggc accgtggaaa acgccatgaa cctgcttaat atttctcctt tcgcgcagta
     4021 cgtggttcgc ggcttaatcc tgctggcagc ggtgatcttc gaccgttaca agcaaaaagc
     4081 gaaacgcatt gtctgatgct tttttccaca acaatttaac gttttttccc accacagcca
     4141 gccgccacaa cggttggctg ttcttcattg caaatggcga cccccgtcac actgtctata
     4201 cttacatgtc tgtaaagcgc gttctgcgca aaacaataag aaaagagaag gaggagagcc
     4261 gggtgacaga accgttaacc gaaacccctg aactatccgc gaaatatgcc tggttttttg
     4321 atcttgatgg aacgctggcg gaaatcaaac cgcatcccga tcaggtcgtc gtgcctgaca
     4381 atattctgca aggactacag ctactggcaa ccgcaagtga tggtgcattg gcattgatat
     4441 cagggcgctc aatggtggag cttgacgcac tggcaaaacc ttatcgcttc ccgttagcgg
     4501 gcgtgcatgg ggcggagcgc cgtgacatca atggtaaaac acatatcgtt catctgccgg
     4561 atgcgattgc gcgtgatatt agcgtgcaac tgcatacagt catcgctcag tatcccggcg
     4621 cggagctgga ggcgaaaggg atggcttttg cgctgcatta tcgtcaggct ccgcagcatg
     4681 aagacgcatt aatgacatta gcgcaacgta ttactcagat ctggccacaa atggcgttac
     4741 agcagggaaa gtgtgttgtc gagatcaaac cgagaggtac cagtaaaggt gaggcaattg
     4801 cagcttttat gcaggaagct ccctttatcg ggcgaacgcc cgtatttctg ggcgatgatt
     4861 taaccgatga atctggcttc gcagtcgtta accgactggg cggaatgtca gtaaaaattg
     4921 gcacaggtgc aactcaggca tcatggcgac tggcgggtgt gccggatgtc tggagctggc
     4981 ttgaaatgat aaccaccgca ttacaacaaa aaagagaaaa taacaggagt gatgactatg
     5041 agtcgtttag tcgtagtatc taaccggatt gcaccaccag acgagcacgc cgccagtgcc
     5101 ggtggccttg ccgttggcat actgggggca ctgaaagccg caggcggact gtggtttggc
     5161 tggagtggtg aaacagggaa tgaggatcag ccgctaaaaa aggtgaaaaa aggtaacatt
     5221 acgtgggcct cttttaacct cagcgaacag gaccttgacg aatactacaa ccaattctcc
     5281 aatgccgttc tctggcccgc ttttcattat cggctcgatc tggtgcaatt tcagcgtcct
     5341 gcctgggacg gctatctacg cgtaaatgcg ttgctggcag ataaattact gccgctgttg
     5401 caagacgatg acattatctg gatccacgat tatcacctgt tgccatttgc gcatgaatta
     5461 cgcaaacggg gagtgaataa tcgcattggt ttctttctgc atattccttt cccgacaccg
     5521 gaaatcttca acgcgctgcc gacatatgac accttgcttg aacagctttg tgattatgat
     5581 ttgctgggtt tccagacaga aaacgatcgt ctggcgttcc tggattgtct ttctaacctg
     5641 acccgcgtca cgacacgtag cgcaaaaagc catacagcct ggggcaaagc atttcgaaca
     5701 gaagtctacc cgatcggcat tgaaccgaaa gaaatagcca aacaggctgc cgggccactg
     5761 ccgccaaaac tggcgcaact taaagcggaa ctgaaaaacg tacaaaatat cttttctgtc
     5821 gaacggctgg attattccaa aggtttgcca gagcgttttc tcgcctatga agcgttgctg
     5881 gaaaaatatc cgcagcatca tggtaaaatt cgttataccc agattgcacc aacgtcgcgt
     5941 ggtgatgtgc aagcctatca ggatattcgt catcagctcg aaaatgaagc tggacgaatt
     6001 aatggtaaat acgggcaatt aggctggacg ccgctttatt atttgaatca gcattttgac
     6061 cgtaaattac tgatgaaaat attccgctac tctgacgtgg gcttagtgac gccactgcgt
     6121 gacgggatga acctggtagc aaaagagtat gttgctgctc aggacccagc caacccgggc
     6181 gttcttgttc tttcgcaatt tgcgggagcg gcaaacgagt taacgtcggc gttaattgtt
     6241 aacccctacg atcgtgacga agttgcagct gcgctggatc gtgcattgac tatgtcgctg
     6301 gcggaacgta tttcccgtca tgcagaaatg ctggacgtta tcgtgaaaaa cgatattaac
     6361 cactggcagg agtgcttcat tagcgaccta aagcagatag ttccgcgaag cgcggaaagc
     6421 cagcagcgcg ataaagttgc tacctttcca aagcttgcgt aggagcggtt aatctcccgt
     6481 aagtggaact aacagcacat cgacctcact tgaggcaatc actcttttcg ccgagcagga
     6541 tgctcgcgaa aagaaactgt gattgtgatt accgcaaatc accaaatcga aatgatgttt
     6601 gtgacatact tccagaatat gttcgcttaa ctcaccgtag gcaataaacg tcttgtcaac
     6661 gggatacccc gcatcctgaa tgaacttatc aagaaagctt tgcgtttctt cttgcatcac
     6721 gctgcgtaaa tcttcaagca tcggcgcggc taattgattg tacatttccg ggtcggaagc
     6781 gagagtaatt aaactgatgt gtccctttac tggcctggcg atagataccg cttttgccag
     6841 cagttgctga ctttccggtg taaccgcaac agcgacaaga atattgctat agctcataac
     6901 ctgttcctta ttctgtgaac ttcaggtgac attaaagctt tactcctaat ggagaaacga
     6961 cgcaatccca actcggtcaa acaggtaaat attagtctga ttattagtaa agacaattaa
     7021 tca
//
LOCUS       DAEEKQ010000193         6860 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.195, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000193 DAEEKQ010000000
VERSION     DAEEKQ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6860
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.195"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(309..794)
                     /gene="sixA"
                     /locus_tag="KLK57_003684"
     CDS             complement(309..794)
                     /gene="sixA"
                     /locus_tag="KLK57_003684"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HBE0893950.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
     gene            complement(997..3141)
                     /gene="fadJ"
                     /locus_tag="KLK57_003685"
     CDS             complement(997..3141)
                     /gene="fadJ"
                     /locus_tag="KLK57_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HBE0893951.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3141..4451)
                     /gene="fadI"
                     /locus_tag="KLK57_003686"
     CDS             complement(3141..4451)
                     /gene="fadI"
                     /locus_tag="KLK57_003686"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HBE0893952.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLCDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4631..4915)
                     /locus_tag="KLK57_003687"
     CDS             complement(4631..4915)
                     /locus_tag="KLK57_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HBE0893953.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5287..6627
                     /gene="fadL"
                     /locus_tag="KLK57_003688"
     CDS             5287..6627
                     /gene="fadL"
                     /locus_tag="KLK57_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HBE0893954.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1716 a         1833 c         1625 g         1686 t
ORIGIN      
        1 aatatataaa cactatagcc agaatttaat caagtttcaa ttacatcctg cctgattatt
       61 caactctatt ttctctatta tctgtattaa taaatgacaa tgagtgtcaa acagacatta
      121 ttcatttcaa catgtaactt tgggcaaaat caagatacgc gcagaagaat aatttccgaa
      181 tgaaagtaga aagtgtttta tttatagatg gggagagtct tcgatagtag tgagattgac
      241 agatatccgc tgcctgatga gcaattatgc ccaatgaatc tacctcattg ggcataattt
      301 ggaactcatc agatagcttt tgccatcttc agattacacg ggctcatctg ccagttgaat
      361 gtgccgtttc cgctttcatc aagcgttacg ctggcaatgg ccgaggtggt aaacataggc
      421 ggcgtttcgc ccggacataa ctcggcaacc agatacccga ctaacggtaa gtgagagatc
      481 accagtaccg aagcaacacc ttcattggtc aacgcctgca aataggcact gaccagacca
      541 acatcgccgc agggcgttaa ctccggcaga acttctgcac tggaaggcag gttcagacaa
      601 tcccctactt cttccagtgt ttgctcggct cgcaggaacg ggctcaccag aacgcgttcg
      661 atttccactt tttgaccttt cagccagttc gccatcaggc gagattcgtc acaaccatta
      721 gtggtcagag gacgaaccga atcactggcg gcatcaaggg ctgcgtcgcc gtgacgcatg
      781 ataaaaactt gcatattgca ccgcttttgt taaccagatt cgtccgtttt ctttcatctg
      841 aaaccagaga aagaaaacgg tggccggcat tgtgccttat ccattcaccg aatgaaacgc
      901 tgttttttac ctcaatggcg taagtatagt caatatgcgt ttacattttg accaacactc
      961 cgccattcag cgcggattca tatagctttg accttcttat tgaaggtcag ttgcagttgt
     1021 tttccaaaaa ctttccccac gcgcgcccat ctcgaccaaa cgctcgcaag gggtaaaacg
     1081 ggaaccatac tgcgtggcca gtcgttgcat tattgcaacc acttcgcccg cgccgagaga
     1141 atcgatatag cggaacggtc caccgagaaa tggcggaaaa ccaatgccaa ataccgcgcc
     1201 aatatccccg tcacgcacgc tacggataac ctgctcatca acacaacgta ctgcttcatt
     1261 cagcatcaac atcacacacc gttcagcaac ctgcggtgcg gagagtcgcc cctgcccttg
     1321 tgcgccaatg agcgggtaaa tggcgggatc gacctgtttt ttgcttttac gccctttctg
     1381 accataaaga tagaaacccc gaccattttt tctgcctttg cgatcgtcgt tcaaaattga
     1441 agaaacaaca tttgcaggcg cgctaaaacg ttctccataa gcggcttcca gtacaggaat
     1501 aattttagtc ccggtgtcga ttcctacctc atccaaaagt tggattgggc ctaccggaaa
     1561 accaaatttc actagcgcgg catcaatgtg ctctacccgt tcaccttcgg tcagcatgcg
     1621 gatagcttca ttaatgtaag gcgctaagat gcgattgacg taaaacccgg ctttgtcacg
     1681 cacgacaatg ggcgttttgc cctgtttttt cgccagtttt accgtagtgg cgatggtttg
     1741 cgccgatgta cccgcatgag gaataatctc taccagcggc attttttcca caggactgaa
     1801 gaaatgcagg ccaataactt gctcaggtcg cgcggcgtga gcggcgatat caccaatcgg
     1861 taaagatgac gtgttcgaag caaaaatggt atgagcggcg caattttgct ccacttccgc
     1921 caccatctgt tgttttaatt cgagattttc aaacaccgct tcaataatca gatcgcgatg
     1981 ggcaaagccg cgatagtccg tcgttccgga gattaatgcc agctgtttgt cacgttcgct
     2041 ggctttgaga tgacgacggc gaactttgcc ctccagctga tcccaactgt acttcagcgc
     2101 atgatttatg ccctgcgggt tgatatcttt aattctgacc ggaagccccg ctttacaagc
     2161 agtgacataa gcaataccgc cgcccatcaa gccaccacct aaaattccca cgctgtttaa
     2221 tggcgcaggc ggtgcatcac taccgggatc tttcttcacg tccgtactgg caaaaaagat
     2281 actacgcaaa gcttgcgatt gcggcgtcat cgccagttcg ccaaaggcgc gagcttcagc
     2341 gtcatagccg ctgctggtgc cctgcgctaa tcccgtttca acaacctcca ggatgcgttc
     2401 tgtcgccgga taattgcctt gagttttgtg ttctgttttc ttgccgacca ttttgaacag
     2461 cagcgcacga cctaacggcc ccgccagaat acgctcgcgt acaggtagag ggcgggaaga
     2521 tgggcgatcc ttcttcgcca gctcaacagc ggcttccagc aaaatggagt gcggaacaac
     2581 gtcatccacc agccccagct ttaatgcctg tttcgcccga agttgttttc cggtgagaat
     2641 catctctaat gctgtactga cgcctatcag acgcggtaaa cgctgggtgc cgcctgaacc
     2701 gggtaacaat ccaagttgta cttcaggcaa accgagcacc gttttaggat cgtcagtaca
     2761 aacgcgaccg tggcacgcca gcgccagctc cagcccaccg cccaggcaag cgccatgaat
     2821 agccgcgata accggaatgg gcaaagcatg aatctccgcc atcaactgtt ggccctgccg
     2881 cgccagtgct tccgcttctt gcgccgtttt gcagttgcca atcatgttga tgtctgctcc
     2941 ggcgataaaa ttgtccggtt tcgcggagat aaacaccacg ccgcgcaact ctttgttttc
     3001 acggagttgc ttaataatgg cgcgcacctg cgaggcaaat tccgccttca gggtattcat
     3061 tttctcatcc ggtacatcga tggtgatgat agcaatgttg tccagacgaa cattaagagt
     3121 aaacgctgat gccatttcca ttattccgcc tccagaacca ttgccgcgcc aagcccaccg
     3181 gcagcacagg cggtaactaa accaaatcca ccgccgcgac ggcgaagttc atgcaatgtc
     3241 tgggtaatca tccgcgcgcc ggtcgccgcg aagggatgcc cgtaagcaat cgaaccgcca
     3301 agcacgttaa atttgctatc gtccacttcg ccagtggcat gtgcacgccc cagtacttca
     3361 cgagcaaaac gttcactacc cagcaactga atattcgcca gtgtctgagc tgcaaaggct
     3421 tcgtgcatat cgatcaatgt cagttcgctc atcgtcaaac cggcacgttc cagcgccagc
     3481 ggtgttgacc aggctggacc gagcaacatg tcctgccaga catcaatcgc agtaaatgcg
     3541 tagctgcgca gataccccag cggcaccagc cctaattctt tcgcccggga ttcagtcatc
     3601 aggatcaccg ctgccgcgcc atcggtcagc ggcgtactgt ttgccgccgt taccgttccg
     3661 tgtttgcgat caaacgccgg gcgcagcttt gcgtaatcgg caagcgagga attaccgcga
     3721 atattgttgt cttcgacaag cggttgttta taaggaggga taaaggcagt catcacctct
     3781 tctttgagtt ttccgtctga ccatgcctga gcggcacgct gatgcgaacg gtgcgctaat
     3841 gcatcttgct gttctcgggt gatgccgtag gttttcgcca tttgttcggc ggtgtcgccc
     3901 atccgcaagc cagtagaata ttctgctacc gcaggcggta cgggcattaa gtcgcacaaa
     3961 cgcaggcgag agaagagttt caggcgctgg ctcatggttc gagctttgtt gacatcaacc
     4021 agcacgcgcg ccagtttttt actgacgcca attggcaata cggaagagga atctgcccca
     4081 ccggcaatcc ccgctcggat agttcccgcc atcaggcttt ctgcgacgtt tgcaaccgcc
     4141 tggaaactgg tagcgcaagc gcggctgacg ctgtaagcat cggtatgcac attcattccg
     4201 gtaccgagaa caatttcacg cgcaatgttg ggggcttcag gcatttgtac gacctgacca
     4261 aagaccagct gttcaatcac ttcagcgggg atctcgctgc gtgccagcag ttcgcctacc
     4321 accatcttcc ctaaatcaac cgcgggaatg ccatgaaaag ccgtcgcctg gcgggcaaaa
     4381 ggcgtacgta aaccgctaac aatggcgata cgatcgccct ggcgggtaac cagcggtaaa
     4441 acctgaccca taacactccc ctgtaaaaaa ataaataaag tggtctgacc tgatcatagt
     4501 cttaactatt tttttacatt tagccaagcg gagaaaaagg aaagtgggag ctatgacaca
     4561 gtgaagacag gagaaagaaa agaaaacgcc cctgccagca actgacaggg gcgaacgtac
     4621 ccgagaggaa ttaacgcaga cccagctgga agatcagcat ttcagcttcg caggcaaaag
     4681 tgaaatcgat atccaggcgc acaccgtcag actcttcagt gaaagtcggg gagattttgc
     4741 acggttcgga ttccacgcta cgggcttttt cagtcagcgc cgccagcgtt tgttctgctt
     4801 ctgcgcggtt tgcaaacacg cggctgtaag acgcggtgca gtcggagttg tccataatgg
     4861 tgccaacatc catacagcag caaaccgggg tttcatcagc actacattta ctcatcgttg
     4921 atttcctctg tatgtgcacc caaggtgcca gataaacgtt gtggatattt tacgcttccg
     4981 gaaagtgctg ctccagttgt taattctgca aaatcggata agtgaccgaa atcacactta
     5041 aaaatgatct aaaacaaaat tcacccgaat ccgcgagtgc gcaacctcca aattttgcca
     5101 gctggatcgc gtttcttaga tcatatttga aaaaagatgg aaacatactt gcagcattcc
     5161 agctggtccg acctatactc tcgccactgg tctgatttct aagatgtacc tcagacccta
     5221 cacttcgcgc tcctgttaca gcacgtaaca tagtttgtat aaaaataaat cattgaggtt
     5281 atggtcatga gccagaaaac cctgtttaca aagtctgctc tcgcagtcgc agtggcactt
     5341 atctccaccc aggcctggtc ggcaggcttt cagttaaacg aattttcttc ctctggcctg
     5401 ggccgggctt attcagggga aggcgcaatt gccgatgatg caggtaacgt tagccgtaac
     5461 cccgcattga ttaccatgtt tgaccgcccg acattttctg cgggtgcggt ttatattgac
     5521 ccggatgtga atatcagcgg aacgtctccg tctggtcgta gcctgaaagc cgataacatc
     5581 gcgcctacgg catgggttcc gaacatgcac tttgttgcac cgattaacga ccaatttggt
     5641 tggggcgctt ctattacctc taactatggt ctggcaacag agtttaatga tacttatgca
     5701 ggcggctctg tcgggggtac aactgacctt gaaaccatga acctgaactt aagcggtgcg
     5761 tatcgcttaa ataatgcatg gagctttggt cttggtttca acgccgtcta cgctcgcgcg
     5821 aaaattgaac gtttcgcagg cgatctgggg cagttggttg ctggtcagat tatgcaatct
     5881 cctgccgggc aaactcctca agggcaagca ttggcagcta ccgccaacgg tatcgacagt
     5941 aataccaaaa tcgctcatct gaacggtaac cagtggggct ttggctggaa cgccggaatc
     6001 ctgtatgaac tggataaaaa taaccgctat gcactgacct accgttctga agtgaaaatt
     6061 gacttcaaag gtaactacag cagcgatctt aatcgtgcgt ttaataacta cggtttgcca
     6121 attcctaccg cgacaggtgg cgcaacgcaa tcgggttatc tgacgctgaa cctgcctgaa
     6181 atgtgggaag tgtcaggtta taaccgtgtt gatccacagt gggcgattca ctatagcctg
     6241 gcttacacca gctggagtca gttccagcag ctgaaagcga cctcaaccag tggcgacacg
     6301 ctgttccaga aacatgaagg ctttaaagat gcttaccgca tcgcgttggg taccacttat
     6361 tactacgatg ataactggac cttccgtacc ggtatcgcct ttgatgacag cccagttccg
     6421 gctcagaatc gttctatctc cattccggac caggaccgtt tctggctgag tgcaggtacg
     6481 acttacgcat ttaataaaga tgcttcagtc gacgttggtg tttcttatat gcacggtcag
     6541 agcgtgaaaa ttaacgaagg cccataccag ttcgagtctg aaggtaaagc ctggctgttc
     6601 ggtactaact ttaactacgc gttctgataa cgcgttcgcc tggataaagt cacctgcata
     6661 gcaggtgact ttaactcccc cacttcaccg aaagtagtgt cctcattgct tacctcctga
     6721 gttttgcaaa cagcctgttg gcagcttgcc ctttcaaatc aataagcggt atccacagac
     6781 aaacctgaag ggaaaggcat attttcaggc gttctgctcg tccttctcaa agagtttact
     6841 tttctgcatt tccaggatac
//
LOCUS       DAEEKQ010000194         6795 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.196, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000194 DAEEKQ010000000
VERSION     DAEEKQ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.196"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            356..981
                     /gene="gstB"
                     /locus_tag="KLK57_003689"
                     /pseudo
     CDS             356..981
                     /gene="gstB"
                     /locus_tag="KLK57_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
     gene            complement(978..2093)
                     /gene="yliI"
                     /locus_tag="KLK57_003690"
     CDS             complement(978..2093)
                     /gene="yliI"
                     /locus_tag="KLK57_003690"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HBE0893955.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVTDFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKHYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="KLK57_003691"
     CDS             complement(2204..2587)
                     /gene="bssR"
                     /locus_tag="KLK57_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HBE0893956.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2800..4125
                     /gene="rimO"
                     /locus_tag="KLK57_003692"
     CDS             2800..4125
                     /gene="rimO"
                     /locus_tag="KLK57_003692"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HBE0893957.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPSETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4172..5493)
                     /locus_tag="KLK57_003693"
                     /pseudo
     CDS             complement(4172..5493)
                     /locus_tag="KLK57_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
     gene            complement(5501..>6795)
                     /locus_tag="KLK57_003694"
     CDS             complement(5501..>6795)
                     /locus_tag="KLK57_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HBE0893958.1"
                     /translation="IAEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAV
                     GIIKLCGLEAVEAVFGVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQ
                     TFTRKIAMVNDFDSSFGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSE
                     FIFCDDAKLNEIEENIWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSA
                     ERGIISPLKFITIAEDIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQ
                     LNEPHFHERFTTIMKENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDD
                     FGTGLSNLKRFYEINPDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVES
                     EDVARELIKLGCVQAQGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         1803 a         1697 c         1552 g         1743 t
ORIGIN      
        1 cgctcggcgc ttcaacggtt tgttccgccg cgaatgccgt tggggcaaaa aggaataaaa
       61 atgcagaacc cgctgcaaga ccacgaagga gagaggagta ttgcgtcata aatgccaccc
      121 gagtatccat tcaaaaaaaa tacgttatat cgccgtatga taagttcggt gattaataac
      181 gcacacacaa acttaaagaa accgtaagtt ggtaaagttt ttaaagttta tgcaatatcc
      241 tgccgcttcg ctgcttctgg tgtcattttt ttgccagctg ttgcttaaaa gttaacttta
      301 cttcaccatc ggagtcctgt tcagcctgtg gctgcctgtc ggaaaagggg tgagtatgat
      361 tacgctgtgg ggtcggaata attcaactaa cgtaaaaaag tattgctgac gctcgaagaa
      421 ctggaactac cttatgagca aattctcgcg ggccgtgagt ttgggataaa tcacgatgct
      481 gattttctgg cgatgaaccc taacgggctg gtgccgttgt tgcgtgacga cgaaagtgat
      541 ctcattcttt gggaatcaaa cgccattgtc cgctatctgg cggcacagta cgggcaaaaa
      601 cgcctgtgga tcgactcacc ggcacgtcgt gcggaagcgg aaaaatggat ggactgggca
      661 aaccagacgc tcagtaatgc tcatcgcggg atcctgatgg gattagtcag aacaccaccg
      721 gaagagcgcg atcaggccgc cattgatgcc agttgcaaag agtgcgacgc cctgtttgcc
      781 ctgctcgatg cggaactggc aaaagtaaaa tggttctctg gcgacgagtt tggtgtgggc
      841 gatatcgcta tcgcaccgtt catctacaat ttgtttaacg ttggcctgac ctggacaccg
      901 cgtccaaatc tgcaacgctg gtatcagcaa ctcactgaac gccccgcagt gcgcaaagtg
      961 gtgatgattc ccgttagcta attgcgtggg ctaactttaa gtaattcccc actggactcg
     1021 tcggtgagaa cgtataaata accgtcgggt ccagtgcgaa catcacgaat tcgctgccct
     1081 ctgtccgtta aaatacggcc atcttctgtc actttgtcgc cgttgacgct catcacaatg
     1141 acatctttat ctttcagcgc gccaataaat aatttttgct gccactgggg gaatttatcg
     1201 ctgttataga aggccatgcc gctcacagcg ggcgaatctt tccagtaaaa aacaggttgc
     1261 tcggtcccgg cgacgatctc ccctttcgct tccggtatct taaagcctga atagttgatt
     1321 ccccaggttg ccagcggcca gccgtagtgt ttgccttttt gcgggatatt aatttcatca
     1381 ccaccgcgcg ggccatgttc attcagccac agtgcattac tccacggatt catcgccatt
     1441 ccttgcggat tacgaatgcc ataagaccag atctcggcgc gcgcaccgga ttcctttata
     1501 aaaggattat catccgggat ttcgccctgg tcggtcagac gcaccagttt gccctgtaat
     1561 ttatccagat cctgcgccgt cgggcgctga ttgttttcgc ccagagcaat aaaaagataa
     1621 cctttaccgt cgaataccag ccgcccgcca aaatggttgc cggtagacag ttttggcatc
     1681 tggcggaaga cggtgcggaa gtcggtcact tttgagagat catcacttaa gcggccataa
     1741 cccacagcag ttccggcttt gccatcatcg ccaacttcgg aataacttaa ccagatgcgg
     1801 cgagactgag caaaatcagg cgctaaaacc acgtccagca ggccgccctg cccgtgcgcc
     1861 caaacgtccg gaactccgga aagcggcgca gataatcctt ttcctgcttg ccagtgacgc
     1921 aactcgccgc cgcgcagagt gattaacata ccgtgattat cgggtaaaaa ggccagtgcc
     1981 cagggatggt cgagtttgtc ttgcagtact tcgacattta ccgttgcagg agccgcccag
     2041 agagcggaag aaagacaaat aaggggcaca aggaaaaagg attgtcgatg cataacgcct
     2101 ccggattgac tctggcttaa agcgtagtca gtggaggaga taacaaattc atttttacaa
     2161 aaacttaaac atgaaggggg agacgctttc tcccccttag ttttcaggcc ttctcaagca
     2221 tggcgtgctt ctgcaggctc tggatactca gcgttaagct catcagacaa ttttcaagct
     2281 tatcggcgtt gacggtaata acagtcgggc aatcatggtg cccactcatc aaacatactg
     2341 cggctgtcgc taatgcttct tcagcatgat gaagagcact ccactcttcc tgatccagat
     2401 gaagattcaa ccgcagcgat ttatcgtgca gttcgcgatt cagtttaaaa aagttatctc
     2461 gtagatgatt gctttcgctg acggacatgt atccttttgc cttcctcagt tgcacgtatg
     2521 cagcgaggtc aacgcgtgcg tcgatcaacc ggttcagcag atcgattcgc tgtctgtcaa
     2581 cgaacatact tcgatcctcc tctttccagc cttgttgcga ttaaatagta tggctatttt
     2641 ttgagcaaaa tgctgtccag gatcaattct tcacgccaaa ttaacaaaat ttacatttcc
     2701 ttcgcctccg ccagcgaatg agattgcgat acaaatttta tccagaactc tgtagaatcc
     2761 ctgccctgac ctttcctgta actgaatatt ttttaagcta tgagcaaagt aactccccag
     2821 ccgaaaatcg gctttgtttc ccttggctgt ccgaaaaacc ttgtcgattc agagcgtatt
     2881 ctcaccgaac tacgcactga aggttatgac gtggtaccga gctatgacga tgcggacatg
     2941 gtgatcgtca acacctgcgg ctttattgac agcgcggtac aagaatcact ggaagccatt
     3001 ggtgaagcgt tgaatgaaaa cggcaaggta attgtgaccg gttgtctggg ggcaaaagaa
     3061 gatcagatcc gcgaagtcca cccgaaagtg ctggaaatca ccgggcctca tagctatgag
     3121 caggttctgg agcacgttca tcactacgtg ccaaaaccga aacacaaccc attcctgagc
     3181 ctggtgccag aacaaggtgt gaaactgaca ccgcgtcatt atgcctatct gaaaatttcg
     3241 gaaggctgta atcaccgctg caccttctgc attattccgt ctatgcgcgg cgacctggtg
     3301 agccgtccga ttggcgaagt gttaagtgaa gcgaaacgtc tggtagatgc gggcgttaaa
     3361 gagattctgg tgatctcgca ggatacttcc gcctatggcg ttgatgttaa acatcgtact
     3421 ggcttccaca acggcgagcc ggtaaaaacc agcatggtca gcctgtgcga acagttatcg
     3481 aaactgggga tctggacacg tctgcactac gtttaccctt atccgcatgt ggacgacgtc
     3541 atcccactga tggcagaagg caaaatcctg ccgtatctgg acattccgtt gcagcacgcc
     3601 agcccgcgca ttctcaaact gatgaagcgt ccgggttctg tagatcgcca actggcgcgc
     3661 atcaaacagt ggcgcgaaat ctgcccggaa ctgaccctac gctcaacctt tattgtcggc
     3721 ttccctagcg agacggaaga agatttccag atgctactcg acttcctgaa agaagcgcgt
     3781 ctggatcgcg ttggctgctt taaatacagc ccggttgaag gtgcagacgc caatgccctg
     3841 cctgaccagg ttccggaaga agtgaaagaa gaacgctgga accgtttcat gcagctgcag
     3901 cagcagattt ccgccgagcg cctgcaagag aaagtgggcc gtgaaattct ggtgattatc
     3961 gacgaagtgg acgaagaagg cgcgattggt cgcagcatgg cagatgcacc ggaaatcgac
     4021 ggcgcggttt atctcaatgg tgaaaccaac gttaagccgg gtgatatcct gcgtgtgaaa
     4081 gtcgagcacg ccgatgagta cgatttgtgg ggtagccggg tttaattaat tcaggccggg
     4141 caaaatcatt tgtccgccct gcgaataatg attactgcca gctaatctga gccctgccgt
     4201 ttttcttact cttatacagc cgctcatccg ccagcatttt caagcgttcg acggtggtac
     4261 attcatttga atgcgctaca ccgatactgg tacttaaatt aatgcttccc acctggaaat
     4321 gaacgatttc ttttagaatc gatagcaact cttccagcgt tccacccgta taaatcaccg
     4381 caaattcgtc accgccgaag cggtagattt tacctttatc taccgggatc tgtgatttaa
     4441 ggattttcac cactttcatc aacacttcat cgcccagcaa atgaccgaag gtgtcgttaa
     4501 tgtttttgaa tttatcgacg tcaaacaggc acaaataacc gcctttttcc ggcagcgctt
     4561 ttaaatcttc gtcaaaggat tttctgccca ttgctttggt caggtcgtcc aggaacgccg
     4621 agttatacat actgtagagt tttttgacat cggcagccga aaagaaaagc ccgccatcca
     4681 gtacgcgttc aaaatggtta ttaatgatgg cttctttctc cacaatacca cgattaatca
     4741 ctctgcgtac cagatagagg caactggtac ccgatatcac tgccaccaat gcggcaaaga
     4801 aaataatcaa tgtacagacc cgcaaataaa catctttttt atacgcaaat acgtacaatc
     4861 cggtcggcaa ataggcgaag tgctccatcg cttccgaaac ggtgaagatg gcgtttttag
     4921 cattatattc atcaccggtt taccgttctt aaatagagag aacatcaggt tctcgctgtc
     4981 catcgccagc gattttgaca gtaaagaaaa acgctccaga ccaagcaaaa atatcagact
     5041 acgattgtga gttaccggat agaggtaata gacgaaggtt ttaccgtcaa aagagataaa
     5101 ccctgtattt ttgacatgtc gatacttcac aacctgcata atatctgcac aataattttt
     5161 ctcgaactcc tttacctgaa tacattcgtc actggaaatc agataaggac cgacatcgta
     5221 caaataagag ggggaaaccg agtcggcgta agctgtgcgc aaaccaacca acagctgttc
     5281 ctccgtggga tttagcgtgt tgataagatc aattgccacg ctacgccgat attcagcaat
     5341 atttgccacc gcaaaagcag tatcaataac ggactgtttc aggctataca cccgttcctt
     5401 taccatttga gtgtagatcc cgcaggcaac tgctgaaatc atgataaaaa tcagcagact
     5461 gactgtaagt acgaacttat tgattctgga catacgtaaa ctactctttt actaattttc
     5521 cacttttatc ccaagcggag aatggcatgg gtttctggta cagatacccc tgagcctgaa
     5581 cacatcctaa tttaattaat tcgcgcgcaa cgtcttcgct ctctacgcct tccgcaatca
     5641 ccgggatccg gttatagcgt gccagatcga aaataatgcg cacaattttt cccgcagtac
     5701 cgaaaatatc gccggtaaat tgcgagtcca cctttatgct gtctggatta atttcataaa
     5761 aacgtttcag gtttgacaaa ccggtgccaa agtcatcaat cgatatccgt accccttgtt
     5821 tacgcagttg ttcaatattg agataaaaat gttcattaat tcgctcgatc acggtttcag
     5881 tgatctcaac acagaggctg ttcgccttca ggccattttc tttcatgatg gtggtaaaac
     5941 gctcatgaaa gtgcggttcg tttaactgcc agggcgaaac attaatatgc agtaagaaat
     6001 catccttcag cgacgcacgc tgactaaaat gtctgaattc acccattgcc gttttaataa
     6061 tctgataacc cagctcattg ataaacccga tatcttcagc aatggtaatg aacttcagcg
     6121 gtgaaatgat cccccgctcc gcagaaaccc aacggcacaa cgcctccgcg cccagaatgg
     6181 cgcgggtgtt aatatcaacg atcggttgat agacgaggaa tagttcgcca atttccattg
     6241 catggcgaat attacgcgca atccagatat tctcttctat ttcgttgagt ttcgcgtcat
     6301 cacagaaaat aaactcggag aacatatggt cacggatact ggaaatcgcc agtttcagtt
     6361 tttctgcgta actccaggcg ttttccccct gcaaaggttc acaaatggca gacttatgga
     6421 tgcgcagatt gcggtagcca aagctgctgt caaaatcgtt taccatcgct attttacggg
     6481 taaatgtctg tacattttcc cgacacagaa gtaaatagag atcggcattg aatgtcacga
     6541 tatcgcagca ttgcgcatat ttctcggcaa ttcgctgaca caatttcctg acgattttat
     6601 tgcgttcatc aacaccaaac accgcttcca cggcttccag accacacagt ttgatgatcc
     6661 ccacagccag atactgatta ttgttggact caataatcgc catattttta ataaaaccgc
     6721 tttcctgatc gtaagagatc agtgagagca atttatttga ccgttcaatt tcggcatctt
     6781 tcatttccgc gatcg
//
LOCUS       DAEEKQ010000195         6774 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.197, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000195 DAEEKQ010000000
VERSION     DAEEKQ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6774
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.197"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(281..424)
                     /locus_tag="KLK57_003695"
     CDS             complement(281..424)
                     /locus_tag="KLK57_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_072020761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin release lysis protein"
                     /protein_id="HBE0893959.1"
                     /translation="MKKITWIILLLLAAIILAACQANYIRDVQGGTVSPSSSAELTGL
                     ATQ"
     gene            complement(522..773)
                     /locus_tag="KLK57_003696"
     CDS             complement(522..773)
                     /locus_tag="KLK57_003696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012644888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin E5 immunity protein"
                     /protein_id="HBE0893960.1"
                     /translation="MKLSPKAAIEVCNEAAKKGLWILGIDGGHWLNPGFRIDSSASWT
                     YDMPEEYKSKIPENNRLAIENIKDDIENGYTAFIITLKM"
     gene            complement(841..2511)
                     /locus_tag="KLK57_003697"
     CDS             complement(841..2511)
                     /locus_tag="KLK57_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-type pyocin domain-containing protein"
                     /protein_id="HBE0893961.1"
                     /translation="MSGGDGRGHNTGAHSTSGNINGGPTGLGVGGGASDGSGWSSENN
                     PWGGGSGSGIHWGGGSGHGNGGGNGNSGGGSGTGGNLSAVAAPVAFGFPALSTPGAGG
                     LAVSISAGALSAAIADIMAALKGPFKFGLWGVALYGVLPSQIAKDDPNMMSKIVTSLP
                     ADDITESPVSSLPLDKATVNVNVRVVDDVKDERQNISVVSGVPMSVPVVDAKPTERPG
                     VFTASIPGAPVLNISVNNSTPAVQTLSPGVTNNTDKDVRPAGFTQGGNTRDAVIRFPK
                     DSGHNAVYVSVSDVLSPDQVKQRQDEENRRQQEWDATHPVEAAERNYERARAELNQAN
                     EDVARNQERQAKAVQVYNSRKSELDAANKTLADAIAEIKQFERFAHDPMAGGHRMWQM
                     AGLKAQRAQTDVNNKQAAFDAAAKEKADADAALITAMESRKKKEDNKRDAEGKLNDEL
                     AKNKGKIPGLKIDQKIRGQMPERGWTEDDIKNTVSNGATGTSFDKRSPKKTPPDYLGR
                     NDPATVYGSPGKYVVVNDRTGEVTQISDKTDPGWVDDSRIQWGNKNDQ"
     gene            complement(2744..4963)
                     /locus_tag="KLK57_003698"
     CDS             complement(2744..4963)
                     /locus_tag="KLK57_003698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBE0893962.1"
                     /translation="MAIYHLSMKIISRKNGYSAVASAAYRSGSVIPDDRTGLIHDYTR
                     KRGVDDAVILTPANAPSWCGDRSVLWNAVEKAEQRRNSQLAREIELAIPREISREAAR
                     ETVLAFVRENFVSRGMIADVAFHHMDRTNPHAHIMLTTRAVGETGFAGKVRDWNDRAL
                     AETWRASWADHANRALANAGYQEEIDHRSYERQGLEKAPGLHLGKAACAMEKRGMETE
                     RGEQNRLINSLNLEIQVSRTQLALRTVQEAQRKRELSDAARRAAEALNLTIPAANASA
                     DTLREFIATLPQECGNAWEMTPEFLAMSGKVNDIEREGNALLKEQAILEKEMTGLKKA
                     RPVASLLSEIPLMTWAEPEYRKRQLRFWKLGKQIESLRRTYRAVKERDIPARRQAFET
                     QWNTWIAPGMAELKEKLSAREAERRREEAEAEARRKEQEHEARLKRHDNHRLSRETAL
                     VGVITELGRAREPGTGRITRYMMLSNRAGEFTVWGDELAHYPQSVHDPVNVYLSPGGA
                     VMVSDIREGMPESHETMARPERVRMYSGATVRHVLEQMRQGWPSYGFPALPHHWPDNF
                     YFSDDRRPVASPLPSAHRVDITADTVPEQLMPVVFSREGNSRTLNLLLCKGPEEMLVG
                     FVRREGGFCPVLVLPSPDYSHLIVSTITENGVCLAGYGEAINHDEDTPYPPEPHLMQF
                     RLKGHHDRLLAAVHKPEEMPDYLFRQLGFNQTWHEWKRDEQHRQQQRRPGHFRGMSM"
     gene            5142..5408
                     /locus_tag="KLK57_003699"
     CDS             5142..5408
                     /locus_tag="KLK57_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mobilization protein"
                     /protein_id="HBE0893963.1"
                     /translation="MSRTLEQKIAEAEARLQRLKAKSRSLDTAQKVIVGAAMLARVRR
                     PEEAQLRAFLLQFLRKEVTRQADVNRIQPLINELEKLPKPPAKP"
     gene            complement(5633..6526)
                     /locus_tag="KLK57_003700"
     CDS             complement(5633..6526)
                     /locus_tag="KLK57_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBE0893964.1"
                     /translation="MSAVLQRFREKLPHKPYCTNDFAYGVRILPKNIAILARFIQQNQ
                     PHALYWLPFDVDRTGASIDWSDRNCPAPNITVKNPRNGHAHLLYALALPVRTAPDASA
                     SALRYAAAIERALCEKLGADVNYSGLICKNPCHPEWQEVEWREEPYTLDELADYLDLS
                     ASARRSVDKNYGLGRNYHLFEKVRKWAYRAIRQGWPVFSQWLDAVIQRVEMYNASLPV
                     PLSPAECRAIGKSIAKYTHRKFSPEGFSAVQAARGRKGGTKSKRAAVPTSARSLKPWE
                     ALGISRATYYRKLKCDPDLAK"
BASE COUNT         1600 a         1870 c         1563 g         1741 t
ORIGIN      
        1 aaaaaaaaca aaaagacaag gaaacaccag ggtaaacata aaaaatgcgt cagaatcgtt
       61 tttagcgcgt tgcataacta tgcacgaaag cgatgcacga tcggtttcgt gcgttctgaa
      121 caccgaacgg ctcaaaacga caagttttct ccccctggat ggaaagttct gcacggggat
      181 tttagatccc atctcccaca tggaattttt gtgacacttc aaaaaaaagc gcgtcagaat
      241 cagcctgaca cgctttgttc ttgtcaaaga ggatttcggg ttactgcgtt gctaatccgg
      301 tcagttcagc tgaggatgac ggggatacag tcccgccctg aacatcgcgg atatagtttg
      361 cctgacatgc agcaagaata atggctgcaa gaagcaataa aataatccat gttatttttt
      421 tcatactgtt ccttgtatat atggactcag gcattcatcc ataaaaacct gaaatccaaa
      481 ggttgaaatc atccatgaaa cataaaattc tataacacta tttacatctt taacgtgata
      541 atgaaagcag tgtatccatt ctcaatatca tctttaatat tttcaatagc caatctatta
      601 ttttcaggga tttttgattt gtattcctcc ggcatatcat atgtccatga tgctgaacta
      661 tctatcctga atccaggatt cagccaatgc ccaccatcaa tgcccaaaat ccataagcct
      721 tttttcgctg cttcattaca aacttctatt gcagcttttg gtgataactt catagaagca
      781 tttcctttta attcaaaaaa ggcatcacca ctatcatata acaccgtatg ttcaaataat
      841 ttattggtca tttttatttc cccattgaat tctcgaatcg tccacccaac ccggatctgt
      901 cttatcacta atctgagtaa cctcaccagt acgatcattg acaacaacat atttaccagg
      961 agaaccatac actgttgctg gatcatttcg acctaaatag tctggaggtg ttttcttcgg
     1021 actacgctta tcaaaagatg taccagttgc accgttagat acggtatttt tgatatcatc
     1081 ctctgtccat cctctttctg gcatttgtcc cctaattttc tgatctattt ttagacctgg
     1141 tatctttcct ttattttttg ctaactcatc atttagtttg ccttcagcat ctcttttatt
     1201 atcctctttc ttcttcctgc tttccatcgc agtaattaat gcagcatcag catctgcctt
     1261 ctcttttgca gcagcatcaa atgcagcctg cttattattt acatccgtct gcgcccgctg
     1321 agctttaagc ccggccattt gccacattct gtgaccgcca gccattgggt catgtgcaaa
     1381 tcgttcaaat tgttttattt cagctattgc atcagcaaga gttttattcg ctgcatcaag
     1441 ttcgctttta cgcgaattat aaacctgaac agctttagcc tgtcgctcct gatttctggc
     1501 aacatcttca tttgcctgat tcagctctgc acgcgcgcgt tcataatttc gctcagccgc
     1561 ttcaaccgga tgcgtagcat cccattcctg ctgacggcga ttttcttcat cctgacgttg
     1621 ttttacctgg tcagggctaa gaacatcact cactgaaaca tatacggcat tatgaccgct
     1681 gtccttcggg aatcgaataa ctgcatccct ggtattaccc ccctgagtaa atcctgccgg
     1741 gcgaacatcc ttatcagtat tatttgtaac acctgggctt aatgtctgta ctgctggcgt
     1801 actgttatta actgaaatat tcagaacagg tgcacctgga attgatgccg taaaaacacc
     1861 tggacgttcg gtaggttttg catcaaccac cggaacactc atcggaacac ctgaaacaac
     1921 cgaaatattc tgtcgttcgt cttttacatc atcaacaaca cgaacattta cgtttactgt
     1981 tgccttatcg agaggtaatg aactgacagg tgattcagta atatcatctg cgggtaatga
     2041 cgtcacaatc tttgacatca tattggggtc atctttcgct atttgtgatg gcaatacacc
     2101 atataaagcc accccccaaa gaccaaattt aaacggtcct ttcagggcag ccataatatc
     2161 agcaatagct gccgataatg ctcccgctga aatactgacc gccagaccgc cagctcctgg
     2221 agtggaaagt gccggaaaac caaatgccac tggcgcagct actgctgaca gattaccgcc
     2281 tgttcccgag ccaccaccgg aattaccatt ccccccgcca ttaccatgac cggaaccacc
     2341 accccagtga atgccgctac cggaaccacc accccacggg ttattttccg aactccatcc
     2401 ggagccatca gaagcaccac cacctacacc aagcccggtc gggccaccat taatgttacc
     2461 acttgtgcta tgcgcgcccg tgttatggcc gcgtccatcg ccaccgctca taaaaactcc
     2521 ctcttttaag cgttaaaaca attaattaca tgattaatac tgtacataaa accactggtt
     2581 ttatgtacag atcaaaaagt acccgtcatc gttttttctg tcaagttcag gtggaaaatt
     2641 ttttagaaat caggagggat aacacttaga aaacgcagag cgggccacaa aaaattttgt
     2701 ggcccgtgat gtgttggcgg ggtccggggt gcagccccgg atatcacata ctcatgcccc
     2761 ggaagtgccc ggggcggcgt tgttgttgcc tgtgctgttc gtcccgcttc cactcatgcc
     2821 aggtctgatt aaaaccgagt tgacggaaga gataatccgg catctcttcc ggtttgtgga
     2881 cagcagccaa aagcctgtca tgatggcctt tgagccggaa ctgcatcagg tgcggttccg
     2941 gtgggtacgg agtatcctca tcatggttta tagcttctcc gtaacctgcc aggcataccc
     3001 cgttctccgt gatggtgctg actatcagat gactgtaatc cggcgacgga agcacaagaa
     3061 cggggcaaaa ccctccttcc cggcgaacaa acccgacaag catttcctcc ggccctttgc
     3121 ataacaacag attcagcgtc ctgctgtttc cctctctcga aaatacaaca ggcatgagtt
     3181 gttccggtac agtatcagcg gtgatatcca cccggtgcgc tgacggcagc ggagaggcta
     3241 cgggcctgcg gtcgtcgctg aaataaaaat tatccggcca gtgatgcggc agcgccggaa
     3301 aaccgtaaga gggccacccc tggcgcatct gttccagtac atgccggacc gtcgcaccgg
     3361 aatacattct cacacgctca ggccgcgcca tcgtctcatg agattctggc attccctcgc
     3421 gtatatccga gaccatcaca gccccgcctg gcgacaggta aacattcacc gggtcatgaa
     3481 cactctgggg gtaatgcgcc agctcatcac cccataccgt gaattctccg gctctgttac
     3541 tcaacatcat gtagcgggtt atcctgcccg ttcccggctc tctggcacgc cccagctccg
     3601 taataacccc gactaatgcc gtttcacggc tcagacggtg gttatcatga cgtttcagcc
     3661 gcgcctcatg ctcctgttcc ttccggcgcg cttccgcttc ggcttcctcc ctgcgccgct
     3721 ccgcttcccg tgctgacagt ttttctttca gctctgccat tcccggcgca atccacgtat
     3781 tccactgcgt ttcaaaggcc tgacgacggg cgggaatgtc ccgttctttc acggccctgt
     3841 aggtgcggcg aagagattca atctgtttcc cgagtttcca gaaacggagt tgtcttttgc
     3901 ggtattccgg ttcagcccat gtcatcaggg gaatctctga cagaagggac gcgacagggc
     3961 gtgctttttt gagtccggtc atctcctttt cgagaatggc ctgctctttc agcagcgcat
     4021 tcccctcacg ttcgatgtcg ttcaccttcc cgctcatcgc caggaactcc ggggtcatct
     4081 cccacgcgtt cccgcattcc tgcggcagcg tggcaatgaa ttcccgcagg gtatccgctg
     4141 aggcattcgc agcgggaatg gtcaggttaa gggcttccgc tgcacgacgt gcagcatcgc
     4201 tgagttcccg cttacgctgc gcttcctgaa ccgtcctgag agcaagctgc gtgcgggaaa
     4261 cctgtatttc caggttaagg ctgttaatca gacggttctg ctcaccgcgt tctgtttcca
     4321 ttccccgttt ttccatcgca caggcagcct ttccgaggtg aaggcccggc gctttctcca
     4381 gtccctgacg ctcgtatgaa cgatggtcta tctcttcctg gtagccggcg ttcgcaagcg
     4441 ctctgttcgc atggtcagcc catgatgcgc gccacgtctc ggcgagtgcc cggtcgttcc
     4501 agtccctgac ctttcctgcg aatcccgttt ccccgacagc tctcgtggtc agcatgatgt
     4561 gcgcatgggg attggtccgg tccatgtgat ggaacgccac atcggcaatc atgccccgac
     4621 tgacaaagtt ttcccggaca aaagcgagaa cggtctcccg tgcggcctcg cgggaaatct
     4681 cacggggaat ggcaagttca atctcccttg ccagctggga gttccggcgc tgttcggctt
     4741 tctcgaccgc attccagaga acggaacggt caccacacca ggacggtgca ttcgcagggg
     4801 tgagaatgac cgcatcatca acgccgcgtt tacgggtgta atcgtggatt aatccggtac
     4861 ggtcatcggg tatgacagag ccggaacggt aggcagcaga agcaacagca ctgtagccgt
     4921 tttttcgcga aatgattttc atgctgagat gataaatcgc catttctgcc gtaatgcctt
     4981 tttgccgttt tcttttagcg tcgcagaccg cacacaatcg aaacgaagtg gagattgtat
     5041 aagtgcgcac ttcgtgatgt gccgtgcatt atgccagttc agttttgcaa ctaccagtga
     5101 cgcgctaaaa tgcgattttt caccgcgcat ggagtcacaa aatgagcaga acactcgaac
     5161 agaagattgc cgaagctgag gccagattac agcgcctgaa ggcgaagagc cggagcctgg
     5221 acacggcgca aaaagtcatt gtgggggctg ccatgctggc gagggtacgg aggccggaag
     5281 aggcgcaact gcgtgcgttc ctgctccagt tcctgcgaaa ggaagtcaca agacaggccg
     5341 atgtgaaccg gatacaaccg ctgattaacg agctggagaa gctacccaaa ccacctgcga
     5401 aaccgtagaa cggagaaacc ccggcgcgta agtgcccatg caccaccgga cgcctttgcg
     5461 cttcctcgct cattcgctcg ctacgctcgg tcatgagact gcggcgagcg ctaccacctc
     5521 aatcatgcgg aaagccacgc cattacgggg cagaacggag gaaatcgcaa gaaagatcac
     5581 tgctcaaaaa gacgccaaaa gggcgctgtt atctgataag gcttatctgg tctcattttg
     5641 cgagatctgg atcacatttt aattttcggt agtacgtcgc tcgactgatc cctaatgcct
     5701 cccacggctt cagcgaacgt gctgatgtag gaactgctgc gcgcttagat ttagttccac
     5761 ccttgcgacc tcgagcggcc tgtacagcgg aaaatccctc tggtgagaat ttcctgtgtg
     5821 tatatttcgc aatgctcttg ccaatagccc gacattcagc cggagaaagc ggaacgggaa
     5881 gcgatgcgtt gtacatttcg acacgctgga tcacggcatc aagccattgt gagaatacag
     5941 gccagccctg acgaatcgct ctgtaggccc atttacggac cttttcgaac agatggtagt
     6001 ttcgccccag cccgtaattt ttatcgacgc tacggcgcgc tgaggcgctc aaatcgagat
     6061 aatcagccag ttcgtcgaga gtgtagggtt cctcgcgcca ttccacttcc tgccattcag
     6121 ggtggcacgg atttttgcag atcaggccgc tgtaattcac atccgcgccc agtttttcac
     6181 acaacgcacg ctcaatagcg gcagcgtatc tgagcgccga agccgatgca tccggcgcag
     6241 ttctcacagg aagggcgagc gcgtagagca gatgcgcgtg cccgttacgg ggatttttta
     6301 cggtgatgtt aggggccgga caattccggt cgctccagtc gattgatgcc cctgtccggt
     6361 ccacgtcaaa gggaagccag tacagtgcat gtggctggtt ctgctggatg aaacgggcaa
     6421 gaatggcaat gtttttcggc agaatgcgaa cgccgtacgc gaaatcgttc gtacagtacg
     6481 gtttgtgcgg taatttttcc ctgaagcgtt gaagtacggc gctcatgtga tacgatttct
     6541 ctcggtacac agtttaacta accgcgcttt cccatcggga aacgcggaac ccggcgccct
     6601 gtaatttcgt gatcaatctt ggcggtgaga gcacgattta cggggcgttt gtttttcagt
     6661 cgcttacact acacccaacg gcaaatcctg tcgatccttc ccgaaaaacc gatccattta
     6721 ccataactcc cgttgtacgt accccttaaa acaccctcaa tcgctttctg acgc
//
LOCUS       DAEEKQ010000196         6661 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.198, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000196 DAEEKQ010000000
VERSION     DAEEKQ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6661)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6661)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6661
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.198"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(78..290)
                     /locus_tag="KLK57_003701"
     CDS             complement(78..290)
                     /locus_tag="KLK57_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncH family protein"
                     /protein_id="HBE0893965.1"
                     /translation="MLCFLIYITLPFSQLVYFISSEKKLTIHIVQMFHLLSQVFYNLK
                     KFLMMDMLGVGDAININANKNIRQVC"
     gene            complement(366..983)
                     /locus_tag="KLK57_003702"
     CDS             complement(366..983)
                     /locus_tag="KLK57_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HBE0893966.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGTSRELL
                     KTLNPLCQIPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWAPDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGSGHEWFQDNATNISAIADAVCQLPKLQEVLKRNEII"
     gene            1250..2749
                     /gene="ansP"
                     /locus_tag="KLK57_003703"
     CDS             1250..2749
                     /gene="ansP"
                     /locus_tag="KLK57_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HBE0893967.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKEGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2864..3925)
                     /locus_tag="KLK57_003704"
     CDS             complement(2864..3925)
                     /locus_tag="KLK57_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HBE0893968.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGVTINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            4167..6269
                     /locus_tag="KLK57_003705"
     CDS             4167..6269
                     /locus_tag="KLK57_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HBE0893969.1"
                     /translation="MKIFSVRQTVLPALLALSPVVFAADEQTMIVSAAPQVVSELDTP
                     AAVSVVDGEEMRLATPRINLSESLTDVPGLQVQNRQNYAQDLQLSIRGFGSRSTYGIR
                     GIRLYVDGIPATMPDGQGQTSNIDLSSVQNVEVLRGPFSALYGNASGGVMNVTTQTGQ
                     QPPTIEASSYYGSFGSWRYGLKATGATGDGTQPGDVDYTVSTTRFTTHGYRDHSGAQK
                     NLANAKLGVRIDEASKLSLIFNSVDIKADDPGGLTKAEWKANPQQAPRAEQYDTRKTI
                     KQTQAGLRYERSLSAQDDMSVMMYAGERETTQYQSIPMAPQLNPSHAGGVITLQRHYQ
                     GIDSRWTHRGELGVPVTFTTGLNYENMSENRKGYNNFRLNSGMPEYGQKGELRRDERN
                     LMWNIDPYLQTQWQLSEKLSLDAGVRYSSVWFDSNDHYVTPGNGDDSGDASYHKWLPA
                     GSLKYAMTDAWNIYLAAGRGFETPTINELSYRADGQSGMNFGLKPSTNDTIEIGSKTR
                     IGDGLLSLALFQTDTDDEIVVDSSSGGRTTYKNAGKTRRQGAELAWDQRFAGDFRVNA
                     SWTWLDATYRSNVCNEQDCNGNRMPGIARNMGFASIGYVPEDGWYAGTEARYMGDIMA
                     DDENTAKAPSYTLVGLFTGYKYNYHNLTVDLFGRVDNLFDKEYVGSVIVNESNGRYYE
                     PSPGRNYGVGMNIAWRFE"
     gene            complement(6305..>6661)
                     /gene="mcbR"
                     /locus_tag="KLK57_003706"
     CDS             complement(6305..>6661)
                     /gene="mcbR"
                     /locus_tag="KLK57_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBE0893970.1"
                     /translation="LTPQDLTELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSN
                     MPILCEMIEQLWVRMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLA
                     EIMQQNIAILYQQYNR"
BASE COUNT         1636 a         1570 c         1646 g         1809 t
ORIGIN      
        1 tagctctgac tcatcaagct ctccatattt atcctataga taattgcgaa taaatacaga
       61 gaacgccatt tatcctttta gcatacctgc cggatatttt tatttgcatt aatattaatt
      121 gcatctccaa ctccgagcat atccatcatt aaaaactttt tgagattata gaatacctgt
      181 gataacaagt gaaacatctg cacaatgtga atagttagtt tcttttcaga ggaaatgaaa
      241 tagacaagct gactgaaggg gagagtgata taaatcaaaa aacataacat gaatttaatt
      301 aattacgccc atcattgtgg ataaagttac tgaataataa ataacataat cctctggtat
      361 cccactcaaa taatttcatt cctttttaat acttcttgta atttcggtaa ctgacataca
      421 gcatcagcaa tcgcactgat attcgtggca ttatcctgaa accattcatg cccagacccc
      481 catgtgcgca tagtgcaaag ataacagtcc accagcgtta gttgctcacc aaacgcataa
      541 ggttcagcgg tcaattgtga attcagccag atataaagcg atttacgata ttcaataaca
      601 ttcttcttta actgttcagg tgcgtcagga gcccaacgct cagggtaatc ggcgaaagtg
      661 aatgttggat agacattagc aaccagccag accaatagcc gttgaaactg ctgacgttcg
      721 gcgcgcccaa ccggtggcgc aaggtccggg cgacgatcga gaaccatcaa tgcaatcgcc
      781 gctgtctccg tcataatctc gtcattttcc agcgccagtg tcggaatctg acacaacggg
      841 ttcagggttt tcaataactc gcgtgaagtt ccctcgtggt caaaaccact gacatcaacg
      901 aactgataag gaatatcagc cagtgttagc atcagctcac tgattgtaga gccccatccg
      961 ggtacgccat acaccttaat catatgcccc tcacaatctt ttaaagatta agtgtaggac
     1021 agtacacagg ttaaccagca aaagtatttg tcattttttc gtcagttgta tgaatcccct
     1081 tcaggattgt aaggggatgt tttgcatcgt atatcgataa cgggtaggat gctgcgccga
     1141 tagttttata cccataaaga ataatggtga taactatcat cgccaggatg aataaacatt
     1201 gttcatggca acttatatga ctttttcatt aaagcaatca gggagagcaa tgagtaaaca
     1261 cgacaccgac acttcagatc aacacgccgc gaaacgccgc tggcttaatg cccacgaaga
     1321 ggggtatcac aaagcgatgg gcaatcgcca ggtacagatg atcgccattg gcggcgcgat
     1381 tggcaccggc ttgtttttag gtgcaggagc ccgactgcaa atggcgggtc cagcactggc
     1441 actggtttat ttaatttgtg gcttgttttc gttttttatt ctgcgtgcat tgggtgagct
     1501 ggtgctacac cgcccttcca gtggcagttt tgtctcttat gcccgtgagt ttttgggtga
     1561 gaaagccgct tatgttgctg gctggatgta cttcatcaac tgggcgatga cggggattgt
     1621 tgatattacc gccgtcgctc tgtatatgca ttactggggt gcgtttggcg gcgtgccgca
     1681 gtgggtcttt gcgctcgctg cacttaccat cgttggcacc atgaatatga tcggtgtgaa
     1741 atggtttgcg gagatggagt tctggtttgc gcttattaaa gtgctcgcca ttgtgacctt
     1801 tttggtcgtg ggtacagtgt tcctcggtag tggtcagccg ctggatggca acaccactgg
     1861 ctttcattta atcaccgata atggcggctt cttcccccac ggtttgctgc ctgcgctggt
     1921 gttgattcag ggcgtagtgt ttgcttttgc ctccattgaa atggtgggta cagctgccgg
     1981 agaatgtaaa gatccgcaga ccatggtgcc taaagccatt aacagtgtga tttggcgtat
     2041 tggcctgttt tacgtcggct ccgtggtgtt gctggttatg ttattgccgt ggagcgcgta
     2101 tcaggcgggg caaagtccgt tcgtgacgtt tttctctaaa ctgggtgtgc catatatcgg
     2161 cagcattatg aacattgtgg tgctgaccgc tgccctctcc agcctgaact caggtctgta
     2221 ctgcaccggg cgtattctgc gctcgatggc gatgggcggt tccgcaccga gttttatggc
     2281 gaaaatgagt cgtcagcatg tgccgtatgc cgggattctg gcgacactgg ttgtgtatgt
     2341 cgtcggcgta ttcctcaact atctggtgcc gtcgcgcgta tttgagattg tgttgaactt
     2401 cgcgtcgctg ggaatcatcg cttcatgggc gtttatcatc gtgtgccaga tgcgcctgcg
     2461 taaagcgatt aaagaaggca aagcagcgga tgtcagtttt aaactgcctg gcgcgccctt
     2521 cacttcctgg ctgacattac tgtttttact gagtgtcctt gtgctgatgg cgttcgatta
     2581 cccgaacggg acttatacta tcgcggcgct gccgattatc ggtattctgc tggttatagg
     2641 ctggtttggt gtgcgcaaac gcgttgctga aattcacagc actgcgccag tcgtcgagga
     2701 agatgaagaa aaacaggaaa ttgtgtttaa gcctgaaacg gcgagctaat tgcaaaattg
     2761 cccggctacg tgccgggcaa atttagatta ctgacgaagg tgtgccgcat gcggcgtgat
     2821 aaaagtcagg caagtctctc cgttttcacc ctcgccatat ctattacagc gcaatacgaa
     2881 tcacatcgtc tggctgggta gcttcctgct gtttagtgga tttttgtttc acactgacat
     2941 acagcgtttt gccatcggca gacagcgcca ggctgtttgg atgagtcggc gtatcgaacg
     3001 ttttcactac tttatagctt ttcgcgtcaa tcacactgac tttacctgcc tgacgatgcg
     3061 ttacgtaggc ttcattacgc gctgggttaa acagcacagc cagtgattcc ggtgctgcaa
     3121 ccttcgccag aatattgcca ttacgggtat cgaccactaa cacttctgcg gctttagaat
     3181 cggtgataaa tgcacgctgc ctagcggtat caaggctaat gttgataaag aagtgctctt
     3241 tgccgtcatc cagcagcttt ttacggctga ggattttatt gtcggcggtg tcgatggtaa
     3301 tcaattcgcc gtcagcgtta gtggtgtaaa gacgtttgcc tttgctatca agcgccagac
     3361 cggtactcat tttaccggtg ttctggatgg cggttttcag tttgatattc tcgccatcaa
     3421 cgacccaaat cacgctctct ttaccaatac cactgatgta aacggtgttc gtggcatcgt
     3481 cagctaccag ctcacgcggt tgcagcgggc gcacctcttc cgtacgctta cgatcatcca
     3541 gcaccagacg gcctttcacc tcgcccgttt tggcatctat cgccgtgacc gcgctgttta
     3601 cagtgttacc aaaccacagt gtctgagtcg tgttattgat ggtgacacca aacggcttga
     3661 gatcgttatg gatcgcctgc gtcacttcca gagtgaccgg atcaagacga taaaccacgc
     3721 cgcctttatc cagtttgcgg ctttgcgaag tggcgagcca cagcgcgttt tcttgctggc
     3781 tataagccat ttcgtaggca cctttaccta ccgctttacg cagcatttct tctgcggcct
     3841 gcgtactgaa tgatgaagca acaagcaatg aacctaacag taatgaacca cgcaggcgcg
     3901 atgaaaacag atgacgtaaa tgcatgacga ctccctttga tgaaccgata tttttgctgc
     3961 ttcacaatgc ttcgctaagc gtaatcatcg ttacgctctt gttataaatg ggaatggtaa
     4021 tcattatttt ctcaaatgtg gagcatttct tgccgttacg actacaattt tctcaattac
     4081 ttaacgtatc gtgccgtttc gttttccatg aatttacaat tttattacca taacggcgtt
     4141 ttcccattct taacctggct tttttcatga agattttttc cgtccgacag accgttttgc
     4201 ccgcactact tgccctttcc cccgttgttt ttgccgctga tgaacagacc atgattgtca
     4261 gtgccgcacc gcaggtggtt tcagaactgg ataccccagc agcagtaagc gtggtggatg
     4321 gcgaggagat gcgcctggca acaccgcgca ttaacttgtc cgaatcactg accgacgtgc
     4381 ctggtttgca ggtacaaaac cggcagaact atgcgcaaga tttacagctg tcgattcgcg
     4441 gatttggctc ccgctccact tacggtattc gcggtattcg cctgtatgtg gacggtattc
     4501 ccgccaccat gcccgacggg caagggcaaa catccaacat cgatttaagc agtgtgcaaa
     4561 atgtggaagt gctgcgtggc cccttctctg ccctgtatgg caacgcgtct ggtggggtaa
     4621 tgaatgtcac cacccagacc ggacaacagc caccaaccat tgaagccagt agttactacg
     4681 gcagttttgg cagctggcgc tatgggctga aagcaacggg cgcaacggga gacggcacac
     4741 agcctggcga tgtcgattac accgtctcaa ccacgcgttt tacgacccac ggctatcgtg
     4801 accatagtgg cgcacagaaa aatttagcca atgccaaact gggcgtacgc attgatgaag
     4861 ccagcaaatt aagtctgatt ttcaatagtg tggatatcaa agcagatgac ccgggtgggc
     4921 taaccaaagc agaatggaag gctaatccac aacaagcgcc tcgtgcagaa cagtacgaca
     4981 cgcgaaaaac catcaagcaa actcaggctg ggttgcgcta tgagcgtagc ctgagcgcgc
     5041 aagatgatat gagtgtgatg atgtatgccg gagagcgaga aacgacccag taccagtcaa
     5101 tacccatggc accacaactt aacccgtcac atgcgggcgg cgtgattacc ctgcaacgcc
     5161 attaccaggg gatagacagc cgctggacac accgtggtga actgggcgtt ccggtcacgt
     5221 tcactaccgg cctgaactac gaaaacatga gtgaaaaccg caagggctac aataacttcc
     5281 gcctgaatag cggcatgccg gagtacgggc aaaaaggtga gttgcgtcgc gacgaacgca
     5341 atctgatgtg gaacatcgat ccctatttac agacgcagtg gcagctgagc gaaaaactgt
     5401 cgctggatgc tggcgtgcgc tacagctccg tgtggtttga ttccaacgac cattacgtta
     5461 ctccgggtaa cggcgatgac agcggtgatg ccagttatca taaatggcta cctgccggtt
     5521 cgttaaaata tgcaatgacc gatgcctgga atatctatct ggcagccggg cgaggttttg
     5581 aaacgccgac gattaatgag ctgtcttatc gtgctgatgg gcaaagcggt atgaactttg
     5641 gtttaaaacc atccaccaac gatacaattg agatcggcag taaaacgcgt attggtgatg
     5701 ggctgcttag tctcgcattg tttcagaccg acactgatga tgaaattgtt gtcgatagca
     5761 gtagcggtgg gcgtacgact tacaaaaatg ccggaaagac ccgtcgtcaa ggcgctgaac
     5821 tggcatggga tcaacgtttc gcaggagatt ttcgcgtaaa cgcgtcctgg acctggcttg
     5881 atgcgaccta tcgcagcaat gtttgcaatg aacaggattg taacggtaat cggatgccag
     5941 ggatcgcccg taatatgggc tttgcgtcga taggttatgt accggaagat ggttggtatg
     6001 caggcacgga agcgcgttat atgggcgata ttatggcaga tgatgaaaat acggcaaaag
     6061 cgccgtctta tactctcgtc ggcttattca ccgggtataa atacaattac cacaatttaa
     6121 ctgtggattt atttggtcgt gtcgataatt tattcgataa agaatacgtt ggttctgtca
     6181 ttgtcaatga gtcaaacggg cgatattacg aaccttcgcc cggacgaaat tatggtgtcg
     6241 gcatgaatat tgcgtggaga tttgagtaat taccttgatg cccggtattt gccgggcatt
     6301 tactttaacg attgtattgc tggtataaaa tagcaatatt ttgttgcata atttcagcaa
     6361 gacaatgtct gcatccctct ttgtcttttg cttttaacgc agcgagtaac agatgatagt
     6421 tttctatatg ctcccgtaat tccgctggat taatcgcttc atagagataa tgtaaaccag
     6481 gccccatcct gacccacagt tgctcaatca tctcacacag gatgggcata tttgagcgat
     6541 gataaatcgc taagcgaaat agcctgttta cattaatgat ttgttccatg tcgccctttt
     6601 cttgcgcctg ctgtaatttc tcgagcaatt cctggagttc cgtcaggtct tgcggggtga
     6661 g
//
LOCUS       DAEEKQ010000197         6593 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.199, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000197 DAEEKQ010000000
VERSION     DAEEKQ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6593)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6593)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6593
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.199"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            144..830
                     /locus_tag="KLK57_003707"
     CDS             144..830
                     /locus_tag="KLK57_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HBE0893971.1"
                     /translation="MSFMAFVTCLTIAFIAGFAVARKLTLRKVASSVIIAKSQPVPSS
                     ATSPAAKASTRSYIPQKTYRDSRWQERDELFQGMVAEGILLERKEWLITRCEKMYFDN
                     LEKWFGHYCHIHCQVSLGRLINFPEQPEFSEEERKRFFAIYNGMAMDYVLVSKKNNKI
                     VCVIELDDPSHERADRIIRDRWLDTIMTLARVPFVRASVTKLEEIPPVWETRDKIRPL
                     VAATKSEPVH"
     gene            complement(921..1057)
                     /locus_tag="KLK57_003708"
     ncRNA           complement(921..1057)
                     /ncRNA_class="other"
                     /locus_tag="KLK57_003708"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(1102..1189)
                     /locus_tag="KLK57_003709"
     tRNA            complement(1102..1189)
                     /locus_tag="KLK57_003709"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1153..1155),aa:Ser,seq:gga)
     gene            1423..2361
                     /gene="ghrA"
                     /locus_tag="KLK57_003710"
     CDS             1423..2361
                     /gene="ghrA"
                     /locus_tag="KLK57_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HBE0893972.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
     gene            2416..3153
                     /gene="ycdX"
                     /locus_tag="KLK57_003711"
     CDS             2416..3153
                     /gene="ycdX"
                     /locus_tag="KLK57_003711"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HBE0893973.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            3177..3731
                     /gene="ycdY"
                     /locus_tag="KLK57_003712"
     CDS             3177..3731
                     /gene="ycdY"
                     /locus_tag="KLK57_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HBE0893974.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDVTEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            3833..4324
                     /locus_tag="KLK57_003713"
     CDS             3833..4324
                     /locus_tag="KLK57_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HBE0893975.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4389..5222)
                     /gene="csgG"
                     /locus_tag="KLK57_003714"
     CDS             complement(4389..5222)
                     /gene="csgG"
                     /locus_tag="KLK57_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HBE0893976.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PTGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQSLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(5249..5665)
                     /gene="csgF"
                     /locus_tag="KLK57_003715"
     CDS             complement(5249..5665)
                     /gene="csgF"
                     /locus_tag="KLK57_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HBE0893977.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            complement(5690..6079)
                     /gene="csgE"
                     /locus_tag="KLK57_003716"
     CDS             complement(5690..6079)
                     /gene="csgE"
                     /locus_tag="KLK57_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HBE0893978.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            complement(6084..>6593)
                     /gene="csgD"
                     /locus_tag="KLK57_003717"
     CDS             complement(6084..>6593)
                     /gene="csgD"
                     /locus_tag="KLK57_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HBE0893979.1"
                     /translation="DDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPE
                     DYPYRDIENWPHINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYN
                     STESALLTHREKEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAV
                     SWANDNLRR"
BASE COUNT         1653 a         1509 c         1634 g         1797 t
ORIGIN      
        1 cgcaatggct aaatagggat ggttgtaata atcaaaacat gatactaact atatattgat
       61 cgttgcattg ttggcatgta acacgctgca gcgagttaca ttggtaatga aatataatca
      121 acatggatag gtgaaaatta attatgagct ttatggcttt tgtaacttgt ttgacaattg
      181 catttatcgc cggttttgct gttgccagaa aattgacact ccgaaaagtt gcctcatcgg
      241 taatcatcgc taaatcgcaa cccgtccctt catctgcaac ttcccctgcc gctaaggcgt
      301 cgacaaggag ctatataccg cagaaaacct atcgtgactc tcgttggcaa gagcgagatg
      361 agttatttca gggaatggtt gccgaaggta ttttattgga aagaaaagag tggttaatca
      421 cgcgttgtga aaaaatgtat ttcgataatc tggaaaaatg gtttggacat tattgtcata
      481 tccattgcca ggtctctctg gggcgcttga tcaactttcc agaacaacct gagtttagcg
      541 aagaagaaag aaagcgtttc tttgcaattt ataatggcat ggcaatggat tatgttctgg
      601 tttcaaagaa gaacaataaa atagtctgtg ttattgagct ggatgacccg agtcatgaaa
      661 gggcggatcg cattattcgt gaccgttggt tggatacaat tatgactctt gcacgggtgc
      721 cttttgtacg agcttctgtc actaaattgg aggaaattcc tccggtctgg gagactcgtg
      781 acaaaattcg cccattagtc gcggcgacaa agagtgaacc ggtgcattaa ctaagtatcc
      841 cggtcggggc ttctcatccc cccggtgtgt gcaatatacg aaaaaaagcc cgtaccttcg
      901 tacgagctct tctttaaaga tggcggtgag ggggggattg actcgcttcg ctcgccctgc
      961 gggcagcccg ctcactgcgt tcacggtctg tccaactggc tgtcgccagt tgtcgaaccc
     1021 cggtcggggc ttctcatccc cccggtgtgt gcaatatacg aaaaaaaagc ccgtactttc
     1081 gtacgagctc ttctttaaat atggcggtga gggggggatt cgaacccccg atacgttgcc
     1141 gtatacacac tttccaggcg tgctccttca gccactcgga cacctcacca aattgtcgtt
     1201 cctgtcttgc tggaacgggc gctaatttag ggaaatcatg acctgaggtc aacaaacttt
     1261 ttgaaaaaat cgcgcgttta ttcaaacttc aatcaatgtg tggttttaat aagcgaaatc
     1321 tgcttttttt gccaccgacc acggatttgt tatgctggtg gcctttgtag atcataacga
     1381 taagtgcgaa taaatttcgc acaacgcttt tcgggagtca gtatggatat catcttttat
     1441 cacccaacgt tcgataccca atggtggatt gaggcactgc gcaaagctat tcctcaggca
     1501 agagtcagag catggaaaag cggagataat gactctgctg attatgcttt agtctggcat
     1561 cctcctgttg aaatgctggc agggcgcgat cttaaagcgg tgttcgcact cggggccggt
     1621 gttgattcta ttttgagcaa gctacaggca caccctgaaa tgctgaaccc ttctgttcca
     1681 ctttttcgcc tggaagatac cggtatgggc gagcaaatgc aggaatatgc tgtcagtcag
     1741 gtgctgcatt ggtttcgacg ttttgacgat tatcgcatcc agcaaaatag ttcgcattgg
     1801 caaccgctgc ctgaatatca tcgggaagat tttaccatcg gcattttggg cgcaggcgta
     1861 ctgggcagta aagttgctca gagtctgcaa acctggcgct ttccgctgcg ttgctggagt
     1921 cgaacccgta aatcgtggcc tggcgtgcaa agctttgccg gacgggaaga actgtctgca
     1981 tttctgagcc aatgtcgggt attgattaat ttgttaccga atacccctga aaccgtcggc
     2041 attattaatc aacaattact cgaaaaatta ccggatggcg cgtatctcct caacctggcg
     2101 cgtggtgttc atgttgtgga agatgacctg ctcgcggcgc tggatagcgg caaagttaaa
     2161 ggcgcaatgt tggatgtttt taatcgtgaa cccttaccgc ctgaaagtcc gctctggcaa
     2221 catccacgcg tgacgataac accacatgtc gccgcgatta cccgtcccgc tgaagctgtg
     2281 gagtacattt ctcgcaccat tgcccagctc gaaaaagggg agagggtctg cgggcaagtc
     2341 gaccgcgcac gcggctacta ataaagcatc aggattcctg ctatccttgg cgggaattga
     2401 atacaggaga gagttatgta tcccgtcgac cttcatatgc ataccgttgc cagcacacat
     2461 gcatatagca cattgagtga ttacattgcc caggccaaac aaaagggcat taaacttttt
     2521 gcgatcaccg atcatggccc ggatatggaa gatgctccgc atcactggca cttcattaac
     2581 atgcgtatct ggccgcgagt ggttgatggg gtagggatcc tgcgcggcat cgaagctaac
     2641 attaaaaatg ttgatggtga aattgattgc agcggtaaaa tgtttgactc gctggatctg
     2701 attattgccg gttttcatga gccggttttt gcaccacatg acaaagcgac caatacacaa
     2761 gcgatgatcg ccactatcgc cagcggcaat gtgcatataa taagccatcc cggaaatccc
     2821 aaatatgaaa tagatgtgaa agccgttgct gaagcagccg cgaaacatca ggtggcgctg
     2881 gaaatcaata attcttcatt tttacactca cgtaagggca gtgaagacaa ctgtcgtgcg
     2941 gtagccgcag cggtacgtga tgctggaggt tgggtggcat taggctcgga ttctcacacc
     3001 gcgtttacca tgggggaatt tgaagagtgt cttaaaatcc tcgacgcggt agattttccg
     3061 ccagagcgca ttttgaatgt ttctccgcgc cgcttactga acttccttga atctcgcggt
     3121 atggcaccga ttgcggaatt tgcagacctt taattactag taatggaaat atattaatga
     3181 acgagttttc tatcctctgt cgtgtgctgg gttcgctcta ttaccgccaa ccgcaagatc
     3241 ctttactggt gccgctgttt accctgattc gtgaggggaa actggctgcg aactggccac
     3301 tggagcagga tgagttactg acgcgtttac agaaaagctg tgatatggcg caagtctctg
     3361 ccgattacaa tgcgttgttt atcggcgatg aatgtgcagt gccgccatat cgtagcgcat
     3421 gggttgagga cgtaacggaa gcggaagtgc gcgcttttct ttccgaacga gggatgccac
     3481 tggcggatac gccagccgat cacatcggca cattgctgct tgcggcttcc tggctggaag
     3541 atcagtcaac ggaagatgag agcgaagcac tggaaacact gttcagtgag tatctgttac
     3601 cctggtgtgg tgcgttcctc ggcaaagtgg aggcccatgc aaccacgcct ttctggcgca
     3661 ccatggcacc gctaacccgt gatgccatta gtgcaatgtg ggacgagctg gaagaagatt
     3721 ctgaagagta aatgtgatca tcgtcactca taactgcaac agataatttg ttgttgcata
     3781 aaatgtgtgc tcgatctcat tcatggccgc gttttctgct atcatgcgcg gcatgaacat
     3841 acttctctct attgcaatca caacaggcat tctctccggt atctggggat gggtggctgt
     3901 ttccctgggc ttactaagct gggcgggctt cttgggctgt acggcctact ttgcctgccc
     3961 gcaaggtggg ctgaaagggc tggcgatctc cgctgcaacc ctgcttagtg gcgtggtgtg
     4021 ggcgatggtc attatttacg gtagtgcact ggcaccacat ctggaaattc tcggttatgt
     4081 cataaccggt attgtggcgt ttctgatgtg tattcaggcc aaacagctgc tgctttcatt
     4141 tgttccgggg acgtttatag gcgcatgcgc gacgtttgcc gggcagggtg actggaaact
     4201 ggtgttacct tcgctggcgc ttgggctggt atttggttac gcaatgaaaa acagcggcct
     4261 gtggctggcg gcgcgtagtg caaagacggc tcaccgtgag caggaaatca aaaataaagc
     4321 gtgaggggca ctcacgcttt cgcttaaaca gtaaaatgcc ggattattaa ttccggcatt
     4381 tttatctgtc aggattctgg tggaaccgac atatggcggt atttaaccag aatgtcattc
     4441 tgccgttctg ctttgttctg caaatcccac agaccacggt cgataccatc attaatcagg
     4501 aaaatgaccc ctgtttcgat agccgacatc agacacagca taacgggttc gtttgaggtg
     4561 taacccactt ccccttccag caagcgctgg tagtcaataa agcggaaaac cccggcctga
     4621 acttcatagg aaagtatcgt cttactggtg ttcaccgagg agaggatctc accggtactc
     4681 acattgacga cacgcaggtt cacggcaatc tgatcgagct ggtattgcgt gtctgcaccg
     4741 atcccaaaat atcttgcccc aacaccgccc gatttaacgt tgctttcata accgataatc
     4801 gaaccttcaa ccatgatatt tgccgccgtt aaagattgca gcgggattcg gttattaatg
     4861 gcaactgtgc cgttttcttg tgccgcacga ataatcttgc gctcgttaag caggttttgt
     4921 aaactctgac gctccagcgg tataaaccag cgagaatctt tcaatgccgt gaccagcatt
     4981 gccgtggcgc tttgcggaac agcagtggag aagttacttg ccgggtaggg tttaaattgc
     5041 ccggtttcgt cctgaatgtt gtataccgaa acaaagatct tacccgtcgg cgctggcaga
     5101 tgggtcaaat ctttgtagct ctgagctcga ggcattaatg tcggtctggc ggcttcttta
     5161 ggcggggcgg ttaagcatcc gctcagtaac atgacggcaa ccaaaagaaa taagcgctgc
     5221 atgattattt tccttatgaa gctggggctt aaaaatcggt tgagttattt tgtaaacccg
     5281 aaacctggat ggtcgaggtt tgtccggttt tacgatctgt cacgttcaac tgcaattgac
     5341 catcgcggtt agcaatatca acaatataat cgttggtcac catgcggccc ggtttaccgg
     5401 tattaatatt cgacagtagc ccacctaaaa tttgtgactg gatggcctga gtaaagttat
     5461 ctaacgctga gggtgtttca ataccaaagt catcgttata gctcggatct ttataagagt
     5521 tttgggcctg agcgctattt aataaaaaag cgccattatt tgggttacca ccaaagtttg
     5581 gattacggaa ctggaaagtc atggttccag cccaacttaa tggcgaaata agcatgagta
     5641 gaactactgc atgtttgaca cgcattgcag cctccgaaca attttttatt tagaattcat
     5701 catgcgccaa atcgcccgta cttaataacg cctgatttat ctggcgacga tttagtgctt
     5761 cttcagtttg aatcagtgca aagacgacag ttttctcgaa gtctcttttc aacggaaata
     5821 aaaaagtctg gaaaataacg tcctgattga ccgttatagt gatccagctt ccccatcgtg
     5881 cactgggcct ttcattaatc gttaagttac ccgtatagtc actttcccat ttatcactaa
     5941 aggctcggta aaaatcatgg ccaatagatg aaacagtatg gtcagttagc aatcccggga
     6001 cttctacctc aacggcgtga agattccctg cggcgaacag aaattctgcc gccacaatcc
     6061 agcgtaaata acgtttcatg gctttatcgc ctgaggttat cgtttgccca ggaaaccgct
     6121 tgtgtccggt tttttacggc tatcttcttg aaaagattat aaagatgcgt tttaaccgta
     6181 ttttcgctga tgaacaacga acgagcgatc tcgttattag acgcgccgat acgcagttta
     6241 ttcaggatct ctttttcccg atgagtaagg agggctgatt ccgtgctgtt ataacgatag
     6301 ttacctgaat gcgtaatcaa gtagctggca agcttttgcg taaagtagca ttcgccgcgc
     6361 aggacgcctt gcaacccatt gacaacacgt tcttgatcct ccatggcata aaaaacgccg
     6421 ttgatatgag gccagttttc aatgtcgcgg tacgggtaat cttcaggcgt atttagcaac
     6481 aatattttga tattgttgtt tttcctgctc aaagtatcct gccaataatg gataagcttt
     6541 ttatccgctt ccatcatatc cagaagaata atagagcctg aagatatatc gtc
//
LOCUS       DAEEKQ010000198         6539 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.200, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000198 DAEEKQ010000000
VERSION     DAEEKQ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.200"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            142..1317
                     /gene="mdtL"
                     /locus_tag="KLK57_003718"
     CDS             142..1317
                     /gene="mdtL"
                     /locus_tag="KLK57_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HBE0893980.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            1292..2251
                     /gene="yidZ"
                     /locus_tag="KLK57_003719"
     CDS             1292..2251
                     /gene="yidZ"
                     /locus_tag="KLK57_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HBE0893981.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            2408..3157
                     /locus_tag="KLK57_003720"
     CDS             2408..3157
                     /locus_tag="KLK57_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HBE0893982.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            3179..3729
                     /gene="yieF"
                     /locus_tag="KLK57_003721"
                     /gene_synonym="chrR"
                     /pseudo
     CDS             3179..3729
                     /gene="yieF"
                     /locus_tag="KLK57_003721"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
     gene            complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="KLK57_003722"
     CDS             complement(3783..5120)
                     /gene="adeP"
                     /locus_tag="KLK57_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HBE0893983.1"
                     /translation="MSHQHTTQTSGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            5286..5951
                     /gene="yieH"
                     /locus_tag="KLK57_003723"
     CDS             5286..5951
                     /gene="yieH"
                     /locus_tag="KLK57_003723"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HBE0893984.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTESEHVYRAEVARLFDSELEAIEGAEALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            6018..6491
                     /locus_tag="KLK57_003724"
     CDS             6018..6491
                     /locus_tag="KLK57_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HBE0893985.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
BASE COUNT         1607 a         1651 c         1652 g         1629 t
ORIGIN      
        1 atttggctaa atccttcaag aagccagcca ttcgctggct tcttgcctct caggaaatca
       61 cttatgtcca aatggcaact cgcctgatcc tccttcacca cgtatgcttt gcgtcacctt
      121 actatcagga cgctttagcc catgtcccgc tttttgattt gtagttttgc cctggtttta
      181 ctttatcccg ccgggattga tatgtacctc gttggtttac cgcgcatcgc cgccgatctc
      241 aatgccagcg aagcgcagtt gcatattgcg ttctccgtat atctggcggg gatggcagct
      301 gcgatgttat ttgccggtaa agtggccgat cgttcaggga gaaagccggt cgccataccc
      361 ggcgcggcgc tatttattat tgcctcggtg ttctgttcac tggctgaaac cagcacgtta
      421 tttcttgcag gccgatttct acaggggttg ggcgcaggct gttgttacgt agtggcgttc
      481 gccattttgc gcgacacgct ggatgatcga cgtcgggcta aagtgctgtc attactcaac
      541 ggtattacct gcatcattcc ggtgttagcg ccagtgctcg gacatctgat tatgcttaaa
      601 ttcccgtggc agagtctgtt ctgggcgatg gcaatgatgg gcatcgcggt actgatgttg
      661 tctttgttta ttttaaaaga aacgcgccca gcgtcccccg ctgcttcgga caaaccacga
      721 gaaaatagcg agtcgctgct taatcggttt ttcctcagcc gtgttgttat caccaccctc
      781 agcgtttcgg tgatcctcac tttcgtcaat acatcgccgg tattgctgat ggaaatcatg
      841 ggttttgagc gcggagaata cgccaccatt atggcgttga ccgctggcgt cagcatgacc
      901 gtttcattct ccacgccatt tgcgctggga atttttaagc cacgtacgtt gatgatcacc
      961 tcgcaggtgt tattccttgc ggcggggatc acccttgccg tttcaccttc ccatgcggtt
     1021 tctctgtttg gtatcacgct gatttgcgcc ggtttctcgg taggttttgg cgtagcgatg
     1081 agtcaggcgt taggaccatt ttcattacgc gcgggcgtag ccagctcgac cttaggtatt
     1141 gcgcaggttt gcggttcgtc actgtggatt tggctggcag cggtggttgg tattggcgca
     1201 tggaatatgc tgatcgggat tctgattgcc tgtagcatag tgagcctgtt gctgattatg
     1261 ttcgtcgcgc ctggacgccc cgttgccgct catgaagaaa tccatcacca cgcttgatct
     1321 caatctgctg ctctgtctgc aactgctgat gcaggagcgc agcgtgacca aagcggcgaa
     1381 gcggatgaac gtgacccctt cagcggtgag taagtcgctg gcaaagttaa gagcgtggtt
     1441 tgacgacccg ctctttgtga actcaccgct gggcctgtcg cccacaccgc taatggtcag
     1501 catggagcaa aatctggcgg aatggatgca aatgagcaat ctgctgctgg ataaaccgca
     1561 ccaccagaca ccgcgcggcc tgaagtttga gttggcggcg gaatcgccgc tgatgatgat
     1621 catgcttaat gcgctgtcga aacggatcta ccaacgttac ccgcaggcga ccatcaaact
     1681 acgtaactgg gattacgatt ccttagatgc cattactcgt ggtgaagtgg atattggttt
     1741 ttccggtcgc gaaagccatc ctcgctcgcg ggagctgtta agctcgctac cgttagccat
     1801 tgattatgaa gtgctgttta gtgatgtacc ctgcgtctgg ttacgcaaag atcatccggc
     1861 actgcatgaa acgtggaatc tggacacctt tttacgttat ccgcatatca gcatttgctg
     1921 ggaacagagc gacacctggg cgctggacaa tgtgttacag gagctggggc gcgaacgcac
     1981 gattgctatg agtctgccgg aattcgagca gtcactgttt atggcagcgc aacccgacaa
     2041 tctgctactg gcgaccgcgc cgcgctactg tcagtactac aatcaactcc atcaactgcc
     2101 gttggttgct cttcctctcc cgtttgacga aagccagcaa aaaaagctgg aagttccttt
     2161 taccctgctg tggcataaac ggaacagcca taatccgaag atcgtctggt tacgggaaac
     2221 cattaaaaac ctttacgcgt cgatggcata accgaatcgt atgaaacgcg acccaatttc
     2281 acaataaaat gtaaaaaagt tgtaacaagc ttgtctgaat cgaactttta gccgctttag
     2341 tctgtccatc attccagtaa atgattactc ttgtattcat aatggaccat taagcatgga
     2401 gcgaaaaatg gcgactcact ttgcccgagg gattttaacg gaaggacatc tgatttctgt
     2461 tcgtctcccc tcccagtgtc atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg
     2521 ttttctggcg tccagaggtt tactcgcaga actgatgttc atgctgtatg gcattggcga
     2581 attgccggaa atcgtcaccc tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc
     2641 ttcgttttct atttcctatg ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga
     2701 atgtggcctc gatatggaac tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc
     2761 cgataaccac accttttcca gcaatgaatc gctatggatc agtaaacaaa acgatcctaa
     2821 cgaagcgcgg gcgcagctca tcacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt
     2881 tttgaatgac gatccgcgcg atctgcagct gctgcccatt gccggacgcc tgaaatgtgc
     2941 tcatgtaaat catgtagaag cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc
     3001 ggtcacgccc gcgattgaaa agctcagtgt ctgggagtta gatggcaaac acagctggaa
     3061 aagcctgccg gatattcaca gccgcgccaa caatcctacc agccggatga tgcgttttgc
     3121 ccagctctct accgtgaagt ctttttcgcc aaattgatag acaaccacag gagtcattat
     3181 gtctgaaaaa ttgcaggtgg ttacgttact ggggagcctg cgcaaaggct catttaatgg
     3241 catggttgca cgtaccctgc cgaaaattgc tccggcgagc atggaagtca atgcgttacc
     3301 ttccattgcc gacattcccc tgtatgacgc tgacgtacag caggaagaag gttttccagc
     3361 aacggttgaa gctctggcgg aacagatccg tcaggctgac ggtgtggtga tcgtcacgcc
     3421 ggaatataac tactctgtac cgggtgggct gaaaaatgcc atcgactggc tttcccgctg
     3481 gccggtaaac cggtgttgat tcagaccagc tcaatgggcg tgattggcgg cgcgcgctgt
     3541 cagtatcacc tgcgccagat tctggttttc ctcgatgcaa tggtgatgaa caagccggaa
     3601 tttatgggcg gcgtgattca gaacaaagtt gatcggcaaa ccggagaagt gattgatcag
     3661 ggtacgctgg accacctgac cgggcaattg accgcatttg gtgagtttat tcagcgagtt
     3721 aagatctaaa taaaaaaccc gccagtaatc atgcatggcg ggtttttaat gcgctatcga
     3781 ttttaatgag cgtcgataaa tacaatcttc aggataaaca gcagcgcaac gatgattacg
     3841 cacgggctaa ggtcacgcag acgcccggta ccaatcttca tcacgcagta ggagataaag
     3901 cccagcgcaa taccttcggt aatcgaaaag ctgaacggca tcatcacggc ggtaataaac
     3961 gccggaacag attcagtaag atcctgccag ttcacgcgag caagacttga ggtcatcaac
     4021 acgccaacgt agatcaacgc gccagctgca gcgtagcctg gcaccattcc cgccagcggc
     4081 gacagaaaga taaccagcag gaacaacaga ccaacaacca ctgccgtcag accggtacga
     4141 ccgccaaccg atacgccgga agaggactca atataagccg taacggaaga agtaccgata
     4201 aacgaaccgg tcacggaaga gatactgtcg acatacagcg cctgcttcat gcgcgggaat
     4261 ttccccttct catccgccag acctgcttta tcggtcacgc caatcagcgt accggaggag
     4321 tcaaacaagt tgaccaacat gaaagagaaa atcacccctg ccagcccgag gttaaacgac
     4381 ccggctaaat ctacatgacc cacaactgtc attacgctcg gcggcgcaga aacgatgcca
     4441 ttgtagtgca catcacccag catccagccc agcagcgtcg tcaccacgat agaaaccagc
     4501 accgctgcgt gaatgttgcg cgaggccaga atggcgatga tgaagaaacc gaggatcccc
     4561 agcagcacgc tgtgagaagt cagattaccg atgctcacca gcgtttccgg gttagcgaca
     4621 atcacacctg cgtttttcag ccccatcatg ccaatgaaca gaccgatacc gctggtaata
     4681 cccacacgca gacttaccgg aatgttagca atcatccagt aacgaacgcg gaaaatggtc
     4741 aaaagaagca gtccgacagc gccccagaag attgcgccca taccgatctg ccacggcagc
     4801 cccattgcct ggacaaccac aaaagcgaaa aaggcgttaa gccccatcgc aggagccagc
     4861 gccaccggaa ggtttgcaaa caatcccatc ataatgctac cgaaagcagc aatcagacag
     4921 gttgttacga agacggcact ggtgtccatg ccagcaacgc ccagaatttg tgggttaaca
     4981 aatacgatgt agaccatcgt caggaaggtg gtgaacccgg caatcacttc agttcgtgcc
     5041 gtagtaccat gttcgcgcag tttaaaaact cgttccagca tcccctgacc agaagtctgg
     5101 gtggtatgtt gatgactcat tatctatttc cgaacaagga gggaaaatcc gtcgctatcg
     5161 tataccaaaa tgcgacaata ggcgcgtttg tgagagactt tttttattgg atttacttat
     5221 acggcaacga ttgcgttgcg caaatcggct ctacgaaaac gttaaactga ttaaaaagga
     5281 aaggcatgtc ccggatagaa gcggtatttt tcgactgcga cggtacgctg gtcgacagtg
     5341 aagtcatttg ctcccgcgca tatgtaacga tgtttcagga atttggtatt acgctcgatc
     5401 ctgaagaggt attcaaacgt ttcaaaggtg taaaactgta cgaaattatc gatattgttt
     5461 cccttgaaca tggtgttacg ttagcgaaaa cagaatctga acacgtttac cgtgcagaag
     5521 tcgctcggct gttcgattca gaactggaag ccatcgaagg ggctgaagcg ctcctgtcag
     5581 cgatcactgc gccaatgtgt gtggtatcta acggcccaaa taacaaaatg cagcattcta
     5641 tgggcaagct gaatatgttg cactacttcc cggataaact gttcagcggc tacgatattc
     5701 agcgctggaa gccagacccg gcgttaatgt tccatgcggc aaaagcgatg aatgtaaatg
     5761 tagaaaactg cattctggtt gatgactcag ttgccggtgc gcaatctggt atcgacgcag
     5821 gtatggaagt gttctacttc tgcgccgacc cgcacaataa gccgatcgtt cacccgaaag
     5881 tcaccacctt tacccatctt tcgcagttac cggaactgtg gaaagcgcgt ggttgggata
     5941 ttacggcata gttcttcaca ctcccttcac ttaccccgct taaattggcg ctcaaaggta
     6001 agtaaaggga gtttgatatg tctgtttcac gtcgggtaat acatcacgga ctttattttg
     6061 cggttttagg accgttaatt ggtgttctgt ttcttgtcct ctacatattc ttcgcaaaag
     6121 aaccgctggt tcttttggta ataatacagg tacttccatt gtttctctta ttgtcgatta
     6181 ctacgggagc tattcctgca ttgttaaccg gcgtgatggt cgcctgtctg ccggaaaaga
     6241 tcgggtcaca gaaaaattat cgttgtctgg ctggtggcat aggtggcgtc gttatcaccg
     6301 agatctattg tgcagttatt gtacatatta agggcatggc ttcctcggag ttgtttgaaa
     6361 acattctttc tggtgacagt ctcgttgtcc gcatcattcc tgcattgctg gcaggtgtgg
     6421 tgatgagcag aatcattacc cgtctgcccg gattggatat ttcatgtcct gaaacagact
     6481 ctttaagtta agcgggatac tttatctttg ggctactcaa aagcagacag gatgttact
//
LOCUS       DAEEKQ010000199         6515 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.201, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000199 DAEEKQ010000000
VERSION     DAEEKQ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6515
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.201"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            38..250
                     /locus_tag="KLK57_003725"
     CDS             38..250
                     /locus_tag="KLK57_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893986.1"
                     /translation="MSNTQKIINTEKYNEWVKKFSEQIFKITGDENVAKNELEPWTPE
                     GNAPNYCWWEVDPVDAANEAMSYHND"
     gene            283..501
                     /locus_tag="KLK57_003726"
     CDS             283..501
                     /locus_tag="KLK57_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBE0893987.1"
                     /translation="MTTLFRKEYPQKSRATEFLFLILFIVLMIPISPLIFVWAIGKII
                     EPVTELYTDVVWASFNTLHNKINPYKEN"
     gene            656..889
                     /locus_tag="KLK57_003727"
     CDS             656..889
                     /locus_tag="KLK57_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001726830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HBE0893988.1"
                     /translation="MVKPATLTVAFKKARDSVDYNWRANGTPPSFHEQRSLSERLFRE
                     QGVDTKILLGHSNQKMTDIYNDVRGKEWKKLVI"
     gene            complement(922..1560)
                     /gene="ompW"
                     /locus_tag="KLK57_003728"
     CDS             complement(922..1560)
                     /gene="ompW"
                     /locus_tag="KLK57_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HBE0893989.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDAGSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDYWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTAKYKSGVTTVKDSVRLDPWVFMFSAGYRF"
     gene            1800..1889
                     /locus_tag="KLK57_003729"
                     /pseudo
     CDS             1800..1889
                     /locus_tag="KLK57_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            1917..2660
                     /locus_tag="KLK57_003730"
     CDS             1917..2660
                     /locus_tag="KLK57_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HBE0893990.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            2690..3229
                     /locus_tag="KLK57_003731"
     CDS             2690..3229
                     /locus_tag="KLK57_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HBE0893991.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            3334..3732
                     /gene="yciA"
                     /locus_tag="KLK57_003732"
     CDS             3334..3732
                     /gene="yciA"
                     /locus_tag="KLK57_003732"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HBE0893992.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="KLK57_003733"
     CDS             complement(3772..4491)
                     /gene="tonB"
                     /locus_tag="KLK57_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HBE0893993.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            4582..6102
                     /locus_tag="KLK57_003734"
     CDS             4582..6102
                     /locus_tag="KLK57_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0893994.1"
                     /translation="MRTVITYLRFSSAIQGAEGADSTRRQNDLFKQWLKKNGDAQIVA
                     SFSDEGLSGYKGKHLTGQFGDMLARIEAGEFPEGTILLVESIDRIGRLEHLETEALMN
                     RILGNGIEIHTLQDGLIYTKDALADDLGISIIQRVKAYIAHQKSKQKSFRVSQKWGQR
                     AKLALAGEQRLTKMVPGWIDPETFKLNEHAETVRLIFKLLLDGESLHNIARHLQSNGI
                     KSFSRRKDANGFSVHSVRTILRSETTIGTLPASQRNDRPAIPNYYEGVVDIPTFNKAQ
                     EILDKNRKGRTPASDNPLTINIFKGLFRCQCGASVHPTGTKNKYAGVYRCNNHLDGRC
                     DVPPLKRKPFDRWMIDNFLGMIDVGNDGESERKIAALQHEVEIVTARIKKATALLLEM
                     DDIDELKIQLKELNQKRTELQTTIDNMRRKASLTDKELPQLKDIDLMTKAGRVECQLI
                     LSKHLKGLTLGKDSVTVTLQNDTEITIPTNPLPLNDGSPIFEIADKELLDIDAYQL"
     gene            complement(6135..>6515)
                     /locus_tag="KLK57_003735"
     CDS             complement(6135..>6515)
                     /locus_tag="KLK57_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0893995.1"
                     /translation="IANGLRIQMTFQGVEFRSIVGQTDVSAATGKTATTNDGGATSKK
                     NTGNTTTSLGSPMLTCKELFGYSASELSDEALKARVTCSKSVSVKNGESTFSDAANEQ
                     ASKVLKPGNALRKYSVNPNKKGTY"
BASE COUNT         1714 a         1489 c         1550 g         1762 t
ORIGIN      
        1 tgtaaataaa atttaatcct taactggagg tatatctatg tcaaatacac agaaaattat
       61 taacactgaa aaatataacg agtgggtgaa aaaattctct gagcagattt ttaaaattac
      121 tggcgacgag aatgtggcaa aaaatgaatt agaaccgtgg acacctgaag gaaacgcacc
      181 aaattattgc tggtgggagg ttgatccggt tgatgctgca aatgaagcca tgagttacca
      241 caacgattaa tgtcgggagg ccgcccgaaa gggcggtaag aaatgactac attattcaga
      301 aaagaatatc cgcaaaaaag tagggcgaca gaatttttgt ttctcattct gtttatcgta
      361 ttgatgatac cgatatcccc tctaattttt gtctgggcaa tcgggaaaat aattgagcca
      421 gttactgaat tgtataccga cgttgtatgg gcgtcgttca acacactgca caataaaatt
      481 aatccgtata aggaaaactg atatggcaac tttgacaaaa aaagaacggg catggttgaa
      541 cgaattacag gaagttcttg atcgctgtcc tgtggtgtca tcctgccgcg attgcgttct
      601 tagtccgtgg ctattgcacc accatcacgc gaaagggaca gctaagcgcg gcgggatggt
      661 taagccagca acattaaccg ttgcatttaa aaaagcccgg gattctgtgg attacaactg
      721 gcgtgctaat ggcaccccac cctctttcca tgagcagaga tctttatcag agcgattgtt
      781 cagagagcag ggggttgata ccaaaatttt gctaggccat tcgaatcaaa aaatgaccga
      841 tatttacaac gatgtacgcg gtaaggagtg gaaaaaactg gtcatttgat aaccagtttt
      901 gcagaggttt tgcagagaaa attaaaagcg gtacccggca gagaacataa acacccacgg
      961 atccagtcgt accgagtctt ttacggtagt aacaccagat ttgtacttag ccgtggtatc
     1021 gatatccatg taccacactg acatgttaac caaccagtca cggttaatca gataatcaac
     1081 ccccacctgc ccggcagctc cccagtaatc tttcagactg agatcggaaa gccccgcctc
     1141 tttgccatga tcgttaaatc cattatcaaa gaaggtggtg tagttaatac ctgccccaac
     1201 ataaggacgg aatttgctgc cggcatcacc aaaataccac tgcgccatca gtgttggcgg
     1261 cagatgatga acggttgcaa tatcgccggt cgcccgggtg ccgattttat ggcggaacgg
     1321 cgtcgctgcc agtaattcca caccaatgtt gtcggtcgcc atataagtaa acgtcaggcc
     1381 cagttgcgtg ttattggtca cgctgaatcc acccagactt cctaacgtac caccagcacc
     1441 ttctgttgga cgtacggttg cagaacctgc acgcataaaa aattcgcctg cttcatgcgc
     1501 aaaggcactg ccagagagaa gagttgttac tgccaaagcc gccactgtta actttttcat
     1561 atccgctccg tcgttatggt tataaaaacg tgacgaatat acctacattg gagtaacaaa
     1621 tgatcctaca tagatcacat tacatatgat aattcaccct ttattgatct ggattaattt
     1681 agaagttgca ttgagaatcc tggttaacgt gatttaaatc aatttttaga tattttcgct
     1741 acgcaaatat taactcgttc gccacttgcc gcggtttgcc cacgcctgaa ggccagcaat
     1801 tacctccgct ttacaaagat atgcttttcc atacaagacc tgatgctgcc acccgaggct
     1861 taaccagggt acaattgccc gctaattaac acctgcataa aactcaagga gagtgcatgt
     1921 ctatcacggc gcagtccgta taccgtgaca ccggaaattt cttccgtaat caatttatga
     1981 ccattctgtt ggtatcgttg ctatgtgcgt ttatcacagt ggtgttaggg catgttttct
     2041 cacccagtga tgcacagctt gcgcagctca atgacggcgt gcccgttagc ggcagtagtg
     2101 ggttgttcga cctggttcag aatatgtcac cggaacaaca acaaattttg ctgcaggctt
     2161 cagcggcgtc cactttttca ggattaatcg gtaacgccat tctcgccgga ggcgtaatat
     2221 taattatcca gctggtgtct gcgggtcaga gagtcagtgc gctgcgagct attggtgcca
     2281 gtgcgccgat attgcccaag ttatttattc tgatttttct gactaccctt ttagtacaga
     2341 ttggcatcat gctggtggtc gttccgggaa ttatcatggc cattttactg gccctggcac
     2401 cggtgatgct ggtacaggac aaaatgggca tttttgcctc gatgcgtagc agtatgcggc
     2461 tgacttgggc gaatatgcgt ctggtggcac ccgcagtact gagctggttg ctggcaaaaa
     2521 cactgttgct gctttttgcc tcttcttttg ccgcattaac cccggaaatt ggtgccgtac
     2581 tggcgaacac cttgagcaac ctgatttcag ccatattgct catctatctg ttccgcctgt
     2641 atatgttgat tcgccaataa ccttaagcat ttgattacgg aatcgtaaaa tgaagcagtt
     2701 tcttgatttt ttaccgctgg ttgtcttttt cgcgttttac aagatttatg acatctatgc
     2761 ggctactgcg gcgctgatcg tcgccacggc gattgtgctt atctatagct gggttcgctt
     2821 tcgtaaggtt gagaagatgg ccctgatcac ttttgttctg gtggtcgtct tcggtggctt
     2881 gacgctgttc ttccacaatg atgagtttat taaatggaag gttacagtca tttatgctct
     2941 gtttgcggga gccctgttag tcagccaatg ggtgatgaaa aagccgctaa ttcagcggat
     3001 gctgggtaaa gaactcacgc tgccgcaacc ggtatggtcg aagctaaatc tggcctgggc
     3061 tgttttcttt atcctttgcg gtctggcaaa catctacatc gcattctggc tgccgcaaaa
     3121 tatttgggtc aactttaaag tctttggcct gaccgccctt accttaatct tcacattgtt
     3181 aagcggtatc tatatctacc gccacatgcc gcaggaagat aaatcctaac cagactgcca
     3241 gtccttcggg gctggcgctg tatctcctct gttctcctca ggaaaatcat agtagcatcg
     3301 cgcctgtgat tttcctttta agtcggtttt accatgtcta caacacataa cgtccctcag
     3361 ggcgatcttg ttttacgtac tttagccatg cccgccgata ccaatgccaa tggtgacatc
     3421 tttggtggtt ggttaatgtc acaaatggat attggcggcg ctattctggc aaaagaaatt
     3481 gcccacggtc gcgtagtgac tgtgcgggtt gaaggaatga ctttcttacg gccggttgcg
     3541 gtcggcgatg tggtgtgctg ctatgcacgc tgtgtccaga aagggacgac atcggtcagc
     3601 attaatattg aagtgtgggt gaaaaaagta gcgtctgaac caatcgggca acgctataaa
     3661 gcgacagaag cattatttaa gtatgtcgcg gttgatcctg aaggaaaacc tcgcgcctta
     3721 cctgttgagt aatagtcaaa agcctccggt cggaggcttt tgactttcta cttactgaat
     3781 ttcggtggtg ccgttaattt taaacaggat attcaccaca atcccactgc ctggcttacc
     3841 cggctcataa cgccatctgc gcatcgcatt tttcacctca cgctcaaaca tgttcgcagg
     3901 cttggctgag aggatttgta cgttatccac gcgaccatcc ggcgtgacgt caaatttaac
     3961 tttaacctgc ccttcaatgc gcaatgcctg tgctcgtgcc ggatactgcg gctgattacg
     4021 gcttaatgcg cgtggtcctg aagccacact ggtaaccggc ttgctggttg cagccgttgc
     4081 cgtacttgat gtggggcgcg cgggtgccgt attttcaaac ggtgatgccg gacgcgactc
     4141 tacgggtttg acatcgcgtt ttggctgctc ctgtaccttt ttcaccggct tcggttttgg
     4201 cttaggtttc ggcttcggct tttcaatgac caccggtgct tcttttggcg gttcggggat
     4261 cggctcaggt tccggttctg gctctaccac cggctccggt ggcggctgaa cggcttgtgg
     4321 cggttcgaga tcagcaggcg caaccatcgt gacagaaatt ggctgcgcag gcgcaggtag
     4381 ttcaataacc tgatgtaccg aggtatagag cagacccgcc acaacagcac catgaatgca
     4441 gaccgaaagt aacgtcggcc aggggaagcg gcgaggtaaa tcaagggtca ttgtattgta
     4501 gctattcggc attgtagcat tagccataac gtttataagt tcatctttaa atgataatca
     4561 attgcatggg agtttatcac cgtgagaacc gtaataacat acttgagatt ctcatcagcc
     4621 atacaaggcg ctgaaggtgc agattcaacc agacgacaaa acgacctgtt caagcagtgg
     4681 ctgaagaaga atggcgatgc tcaaatagta gcgtctttca gtgatgaagg gttgagtggt
     4741 tacaaaggta aacaccttac tggtcagttt ggtgacatgt tagcccgtat tgaggctggg
     4801 gagtttccag aaggtacaat tctgctagtt gagagtatcg accgtatagg ccgattagag
     4861 catcttgaaa cagaagcctt gatgaaccgc atcttaggca acgggataga gattcacaca
     4921 ttgcaagatg gccttatcta cacaaaggat gctttagccg atgaccttgg cataagcatc
     4981 atccagcgtg tcaaagccta catagcgcat cagaaatcta agcagaagtc tttccgtgtt
     5041 tctcagaaat gggggcaacg tgcaaaactt gcccttgctg gtgaacaacg tcttacaaag
     5101 atggttcctg gatggattga ccctgagact tttaaactca atgaacacgc tgagactgtg
     5161 agattgatat tcaaactgct gctagatggt gaaagtctgc ataacattgc acgtcacctt
     5221 caaagcaacg gtataaagtc gtttagtcgc cgtaaagatg ctaatgggtt ctctgttcac
     5281 tctgtacgca ccattctaag gtcagagaca acaataggca cgttaccagc atcacaacgt
     5341 aatgaccgcc ccgctatacc gaactactac gaaggtgttg tagatatacc aacgttcaat
     5401 aaagctcaag agattctcga caagaatcgt aaaggccgta cacctgcaag tgacaaccca
     5461 ctaacgatta acatcttcaa aggtctgttt aggtgtcagt gtggggctag tgtccatcct
     5521 accggaacaa agaataagta tgctggggtc tacaggtgca ataaccactt agacggtcgc
     5581 tgtgatgttc caccgttgaa gcgtaaaccg tttgaccgat ggatgattga taattttctg
     5641 gggatgattg acgtggggaa tgatggagaa tcagagagaa agattgcagc gttacagcat
     5701 gaggttgaaa ttgtcacagc cagaatcaag aaagctaccg ccctacttct tgagatggat
     5761 gatattgatg aactaaaaat tcagcttaag gaactgaacc agaagcgcac agaactacag
     5821 accacgattg ataacatgag gcgtaaggct tcactcactg ataaggaact accccagctt
     5881 aaagatattg accttatgac taaagcgggt cgtgtcgagt gtcagttgat tttgtccaag
     5941 catctaaaag ggcttacatt gggtaaggat tcggtaactg taacgctaca gaacgacact
     6001 gaaatcacca tacctacaaa cccgttaccg ctaaatgatg ggtcaccaat ctttgaaatt
     6061 gctgataaag agctactgga tattgacgct tatcaactgt aattttcagg ggctatgaag
     6121 ccccttttta tgtctcaata agtgcccttc ttgttagggt tcacagagta ctttcgcaga
     6181 gcattacccg gcttaagcac ctttgaagct tgctcattag cggcatctga gaaggtgctc
     6241 tcaccatttt taacgctaac gctcttagag cacgttacac gggcttttag cgcttcatct
     6301 gataactcac ttgccgaata gccaaaaagc tctttacagg tcagcatagg gcttccaagg
     6361 ctcgttgtag tgtttccggt gttcttttta ctggtagcac cgccatcatt cgtggtagcg
     6421 gtctttccag tagcagccga aacatcagtc tgacctacga ttgaacgaaa ctctacacct
     6481 tggaaggtca tctgaatacg aagaccgtta gcaat
//
LOCUS       DAEEKQ010000200         6500 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.202, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000200 DAEEKQ010000000
VERSION     DAEEKQ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6500
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.202"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..492
                     /locus_tag="KLK57_003736"
                     /pseudo
     CDS             1..492
                     /locus_tag="KLK57_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(496..1887)
                     /gene="tcyP"
                     /locus_tag="KLK57_003737"
     CDS             complement(496..1887)
                     /gene="tcyP"
                     /locus_tag="KLK57_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HBE0893996.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQQVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGILALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(2020..2610)
                     /locus_tag="KLK57_003738"
     CDS             complement(2020..2610)
                     /locus_tag="KLK57_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HBE0893997.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2773..3441)
                     /gene="hxpB"
                     /locus_tag="KLK57_003739"
     CDS             complement(2773..3441)
                     /gene="hxpB"
                     /locus_tag="KLK57_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HBE0893998.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLRDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3588..4124
                     /locus_tag="KLK57_003740"
     CDS             3588..4124
                     /locus_tag="KLK57_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HBE0893999.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4165..5025)
                     /locus_tag="KLK57_003741"
     CDS             complement(4165..5025)
                     /locus_tag="KLK57_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HBE0894000.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQLQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(5131..5421)
                     /gene="ghoS"
                     /locus_tag="KLK57_003742"
     CDS             complement(5131..5421)
                     /gene="ghoS"
                     /locus_tag="KLK57_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HBE0894001.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5522..6451)
                     /gene="pfkB"
                     /locus_tag="KLK57_003743"
     CDS             complement(5522..6451)
                     /gene="pfkB"
                     /locus_tag="KLK57_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416237.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HBE0894002.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISASQKQG
                     IRCIVDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1696 a         1609 c         1582 g         1613 t
ORIGIN      
        1 ttaatgagaa ttttgttggg atggatttta cctgtgaatt ccgacccgaa aaaatgacga
       61 atttgatcaa tataaaaaat aggttggtta ttcattattt aaggactata tactatgaaa
      121 aaaattatat acatcctgcc gggagcattt ttgctgcgat tcaaaaaaaa tgagagtctc
      181 ctaaagtttc cttcgataat atcaatgctt aatatcaatc ttctttttaa tccactaaat
      241 cttccgggaa tgggaagtgg tgtgcttgaa gatattatga gtattccaga tagttcactt
      301 agaaaacgtc tcgggtatga ggttttaagc ttctcactac aggcacattc attgagccag
      361 gaatgtattg ataagttgga catttttttc gctgacgatc tgtttaaata tgagtcagta
      421 tgcatcgccg cgatggaaca tttaaaaagt aaagcaacgg cccctataca aaacggaccg
      481 ttgccagcat aagaattaac ggtgtgccag ttcggcgtcg tcttcgctat ccagaatggc
      541 tttatcggtt tgcttcagcc actggctggt cagcgtgcca gctgtcatcg agccactaac
      601 gtttagcgcc gtacggccca tgtcgataag cggttcaacg gagattaaca gcgccaccag
      661 ggttactggc aggcccatcg caggcagcac aatcagtgcg gcgaaagttg caccaccacc
      721 gacaccggca acgcctgcgg aactaacggt aacaataccg accagcgtcg caatccacat
      781 cgggtccagc gggttaatgc caaccgtagg cgcaaccatc accgccagca ttgccggata
      841 caaaccggcg cagccgttct gaccaatggt tgcaccgaaa gaggcggcga aactggcgat
      901 ggattcagga acgcccagac gacgcgtttg tgcttccaca ttcagtggga tagacgcagc
      961 actggaacgg ctggtaaagg caaacgtcag cacaggccat accttacgga agtacttcag
     1021 cggactcacg ccattaatgc ccagcagaat gccatgcact gcaaacataa tcagcagacc
     1081 gaggtaggac gcgacaacga aacttcccag tttgatgatg tcttgcaagt tagaacctgc
     1141 aaccactttg gtcattagag ccagaatgcc gtaaggggtc aactgcatga caaggcgaac
     1201 cagtttcatc acccagcttt gtagggtatc gatagcggct aagacgcgtt cacctttcgg
     1261 cgcatcatcc ttcagcagtt tcaatgcagc tacgccgagg aatgcggcaa aaattaccac
     1321 gctgataatt gacgtcggat tggctccggt aagatcggca aacgggtttt tcgggataaa
     1381 ggacaagacc tgctgcggaa cgctcagatc agagacttta ccaacatagt tactttcaat
     1441 ggcgttcaga cgtgcagttt ctgcaccacc ctgaaccaga ccttcagccg tcaaaccaaa
     1501 caggttggtg accagcacac cgaccagcgc cgcaatcagc gtggtaaaca aaagcgtacc
     1561 gatggtcaga aaactgattt tgcctaactg agatgcgtta tgcagacggg caaccgcgct
     1621 cagaatagag gcgaagacta acggcataac gatcatttgc agcagttgaa catagccgtt
     1681 accaacgatg ttaaaccact gtacagaatc tttaagtacc tggctgtcag aaccataaat
     1741 ggtatgcagg gcaaggccaa aaaccacacc catcaccaga cccaccagca cttttttcgc
     1801 cagactccac tgtttatggc gggtctgagc cagcgcaaac agcagtacaa cgaacaccac
     1861 gatgttcgca attaatggaa agttcatccc cgttctcctg atcttattat ttctatcact
     1921 gcgtagcagt catagtgttg gcgcaagatt aacagaactg tgagctggct cttatattca
     1981 aatggaattg cttattccag aaaggtgatt tttgcctttt tagtattcaa cctgatgctt
     2041 aataagccga tgaaactgga tctgcaaggt attgatcatt tgcgatgacc aacgaacagc
     2101 gctgttctcg ggtaatgaat ggggcatcca taccgaccag acaaataatg ccatgcagat
     2161 aaaacgttcc agttggttgc ccttttgggg aaccaggata ggcaagcgga aacgccagcg
     2221 gcatggccag agcaggggaa caccggcggg tgtcagcata tcggcaagta tgtggctcaa
     2281 ataacccagc accattcctt gtagcgcatc agccggaatg aaccagcctt ccggaacctt
     2341 aaggtaaaag gttgccagca gcgcaaatac cgccagcaga ctgtgggtaa aaccacgatg
     2401 cccaaaagcg cgggcgatcg gttttgatat ccattttaat cgctgcccaa gaaacgactt
     2461 tgggtgatcg atgtccggta acaaacacgt caggattgcg gaagggacaa tatgccacca
     2521 gtcaccctgt gccagcacgg gcgtcagctc ggcatttttg gcaaataccg cacaagcaat
     2581 agaaaagaga aggtgacctt ccgccgtcat gataaaactc actaaactgt cgatacgtac
     2641 agtataggat ttttatacag tgcacggaag tggtgacggt gtaactcttt ctcttaaatt
     2701 gacgattaat ccgtcagttg aatgcaaatt cagataaaga accaggggca gcgtgatcac
     2761 tgcccctggt cattaaccga gaaggtcttt tgcggtgagt tctgtcagcg atgagagttt
     2821 gacgtttgct aatacaaaac gtggatcatt ttgcgcttct ggcgcaggaa cgacgatgga
     2881 acgcatgcgg gctgctttag aagcgatcat gccatttacc gagtcttcca gcgccacgca
     2941 ggtcagtggg tcaacgccca gttttgctgc gcagtcgaga tatacttgcg gatgcggctt
     3001 gctgtaaggc agtttttcag ccgaggcgag ggcatcgaaa ctgtcgcgta agtcaaacat
     3061 ggtcaacact ttttccagca tatgtagtgg tgacgcggag gccagtccca ccaataaacc
     3121 ttgttctttg cataacgcaa cagcttcgcg cacgcctggt aataatggac gtgtctcttc
     3181 aaccagtgaa atggcacggg caataacccg ttctactact tcctgacggc ttggcccatt
     3241 ccacggttgc cgggcgtacc aaagatcgac caccatatcg atgcgtaaac ctaaggtgtc
     3301 cggcagctcg ttacgacggg agatatccac ccccaggctt gccatcacat ccagttcggc
     3361 tcgatcccgt aaaggttctg agtcgataag taatccatcc atatcaaaaa ttgcagcaag
     3421 aatctgacgc ggggttgaca ttacaacctc tcctttagag cattatgtgt aaggtattaa
     3481 gccgtgctta tgattaagcg cctatcatat cgcctttaaa gaatgggcga aaacagatat
     3541 ctacaggtaa actaagtgaa acgacgcgtc ttaatcaagg agaactcatg acgtatcaac
     3601 aagctggacg cattgctgtt ttgaaaagga ttttgggttg ggtgattttt atacctgcat
     3661 tgatctccac attgatttct ttgctgaaat ttatgaatac ccggcaggaa aaccaggaag
     3721 gcattaatgc ggtcatgctc gactttactc atgtcatgat cgatatgatg caggcgaata
     3781 cgcctttttt gaatctgttc tggtataact ccccgacccc taattttaat ggtggcgtga
     3841 acgttatgtt ctgggtgatt ttcatcctga tttttgtcgg actggcgttg caggattccg
     3901 gtgcccggat gagtcgccag gcgcgcttct tgcgggaagg tgttgaagat caactcattc
     3961 tggaaaaagc caaaggggaa gaggggctaa cgcgtgaaca aattgagtcc cgtattgttg
     4021 ttccgcacca tactattttc ctgcagtttt tctcactata tatcctgccg gttatttgta
     4081 ttgctgcagg ttatgtgttc ttttctctgc ttgggtttat ttaatcaaaa ggccgcacgg
     4141 agcggccatt atcctcaacc catatcatgc tgctaataat ctatccaatg actgctgagc
     4201 aataaccaaa tgctgaccgc caaataatct tgcacgattt aacagcgtgt agagttggta
     4261 aaccggttga cgttcaagga aatcggcagg tagcggggat actgactgat agccgtcata
     4321 gatttgtggc ggctgttcag tatgcagcgg taacatcgcc aggtcgcact ctcggtcacc
     4381 ccagtagcag gcagggtcga aaatgtacgg gccatccgga cccagcgcac agttgccgga
     4441 ccataaatcg ccgtgcaaca gagaaggctg cagttgatgc gaggccagac gctgctgaat
     4501 atgctcgacg atggcgtcga tattgccgaa agcgatccct ttctctgctg ccagttccaa
     4561 ctgccagcca atccgttgtt cagcaaaaaa cgttgaccag cgacgttgcc aggtgttggg
     4621 ctgtggagtt gtggagagcg agttatcaaa atcgaggcca aattgtggtt ggtcactcca
     4681 ttgatgtaaa cgcgcaattt gctgaccaag aataaatgcg ctatgcgcat ccagcggacg
     4741 aggtgggaga taatccatca ccagaaaact gtagtcacgg tcagcgccta ctgcccaaac
     4801 cttaggcacg gtgacggttt tactacgcga cagtaactcc agttggtcgg cttcggcggt
     4861 aaaaccggga agcagttccc tttcatcaca tttgacgaaa aagtcatgtc ctgcatagcg
     4921 caaatgccat gcggcatgga cttctccgcc aggcagttca ttacgcagtt cgatttcgcc
     4981 ttcacctaac tgctcgctca aaagacgact gattgcctgc cacatgatgt ctctcccatg
     5041 ttgtctgcca gatcataagg ttagcgcaag aatgcggtgt aaaaacacga gctaacgcac
     5101 accacagact gattattgcc caaaaaccat ttattttctg ttgctattcc attcctccca
     5161 agtcgtgatg gtgatttcag gatctttgcc ggttgcactt tgggtaagcc ccgaaaccag
     5221 ttctctaatt tcttcttcac tttgggtact gataagtcca aaagtgttag tccccagctc
     5281 atggatattt ccctcatcat ccgtcatggt gagcaaaaaa ccgtcgcgag tcaggtaatt
     5341 attcaactcg ttaatttcag tcaatgtatc ctcatgcaac atgacggtta tgacataacg
     5401 gacaagatcg ccactggcca tagttcaccc ctttgttatc acatgacttt tgagcaaagt
     5461 cggagaaacg cgttgccgac aggttggtgg tgattccccc aatgctgggg gaatgttttt
     5521 gttagcggga aaggtaagcg taaatttttt gcgtatcgtc atgggagcac agacgtgttc
     5581 cctgattgag tgtggctgca ctccccgcag ctacgccaaa acggaccatc tcttcaagag
     5641 aggcattttc tgccagtttc agtgtcatcg cgccgaccat gctgtcacca gcgccaacgg
     5701 tactctggct tttcaccggt ggtggcacca cctgaatgca gttttcacta tcaacaccca
     5761 gcgctccttg tggaccaagg gaaacgacaa cccgtttggc cttgccgcta ttaacgattt
     5821 cctgcgcggc tttgcggaca tcgtccggct gggtgagttc gcgattcacc agcgcactga
     5881 gttctttttg gttaggctta accaactcga tgttaccaat tgccagtgct gcacttaacg
     5941 cttcgccaga actgtcgacg atgcagcgga tcccttgttt ttgcgaagcg gaaatcagtt
     6001 gggttaattt ttccagcttc acacctggcg gcaggcttcc gcttatgacc aggatggccc
     6061 cggattcaat ttccagaact tgctcttcaa gctggcgaaa ctcatcttca tttaatgccg
     6121 cgcctggcat aacaaaacga tactgctcac cgcttgcttc cacatgtaca tgtaaattct
     6181 gccgggtcca gtctttggct tctacagtag cgacggggac attttcatcc gccaacagtg
     6241 aaaccaggtg ttcgccggtc gcgccacccg ccgggaagat cgctgtggca ctgcctccaa
     6301 gatgggcaat ggcgcgggcg acgttgatgc cgccgccccc gggttcgaac accggtgcgg
     6361 tacagcgcag ttttccttcg ggataaattt gcggggtaat tgttgcgcta tcgagagagg
     6421 gcgcaagtgt caacgtatag atacgtacca tcatttcctc ctataggctg atttcagtct
     6481 ggcaccgaat cagcggaaag
//
LOCUS       DAEEKQ010000201         6484 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.203, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000201 DAEEKQ010000000
VERSION     DAEEKQ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.203"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..157
                     /locus_tag="KLK57_003744"
     CDS             <1..157
                     /locus_tag="KLK57_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894003.1"
                     /translation="TLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQRTAFKKCLFLM
                     RDIIISS"
     gene            118..237
                     /locus_tag="KLK57_003745"
     CDS             118..237
                     /locus_tag="KLK57_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBE0894004.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(716..982)
                     /gene="minE"
                     /locus_tag="KLK57_003746"
     CDS             complement(716..982)
                     /gene="minE"
                     /locus_tag="KLK57_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HBE0894005.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(986..1798)
                     /gene="minD"
                     /locus_tag="KLK57_003747"
     CDS             complement(986..1798)
                     /gene="minD"
                     /locus_tag="KLK57_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HBE0894006.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(1822..2517)
                     /gene="minC"
                     /locus_tag="KLK57_003748"
     CDS             complement(1822..2517)
                     /gene="minC"
                     /locus_tag="KLK57_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HBE0894007.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            3037..3405
                     /locus_tag="KLK57_003749"
     CDS             3037..3405
                     /locus_tag="KLK57_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgJ family protein"
                     /protein_id="HBE0894008.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQTTTKMLFMCRE"
     gene            complement(3513..3914)
                     /locus_tag="KLK57_003750"
     CDS             complement(3513..3914)
                     /locus_tag="KLK57_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HBE0894009.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            4156..4449
                     /locus_tag="KLK57_003751"
     CDS             4156..4449
                     /locus_tag="KLK57_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HBE0894010.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            4521..5180
                     /locus_tag="KLK57_003752"
     CDS             4521..5180
                     /locus_tag="KLK57_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HBE0894011.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFISAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            5272..5718
                     /locus_tag="KLK57_003753"
     CDS             5272..5718
                     /locus_tag="KLK57_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HBE0894012.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMICAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(5925..>6484)
                     /gene="hlyE"
                     /locus_tag="KLK57_003754"
     CDS             complement(5925..>6484)
                     /gene="hlyE"
                     /locus_tag="KLK57_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HBE0894013.1"
                     /translation="VLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEK
                     SSYFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQS
                     FFTTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAA
                     KKMINTCNEYQKKHGKKTLFEVPEV"
BASE COUNT         1657 a         1448 c         1535 g         1844 t
ORIGIN      
        1 tacattagtc attttatttt gccgacggcc tcattgtcga aagataagcg tacgacagta
       61 ttatctgaaa agagtgattt tttatccaac tacacttcag cgcactgcgt ttaaaaaatg
      121 cctctttctt atgcgggata tcatcatttc atcatgatgt ctttggtgag cggtgaacac
      181 aatacacctg cgctgtctct tcaggatgaa tcccctggtc tggtgcctgc gggctgatgt
      241 tgcagcagag cttaggtcac ttagacgcta ctatcattta tccaatggca tggaatcgaa
      301 atcagtcgat acccgcagta tatatcgtga actggtcgtt ctgaccatca acgattaaac
      361 tgcgcttcgg cgcagttttc gtttacaggg ggctaaaagg gaaaattctg gggcaaaaaa
      421 agcccgccag ttacggcggg aaacctcatc ctatgggaga acaatgaata atgaaattgc
      481 ggggttatca tcccccagtg tatccacact aacaataagg ttatttactc agccaggcat
      541 aaacattatg ttctgtgcgt gggaacagcc ttaaggtgta aagggggagg tggaaatagc
      601 aatgaggagt atcagcaaga atactcgccg ctttaccaca acgtggatga gagggatgaa
      661 aaactcaagg cagaaatgac tctgccttga agataaatgc gtttttacag cgggcttatt
      721 tcagctcttc tgcttccggt aaggtcacgt tcagctcaag aatagaaata tcgccatctt
      781 tttgctcaag ctgtacggtt accatctcag gatcaatttg tacgtattta caaatgacct
      841 caagaatatc tttacgcaac tgcggcagat aatgcggttc tgcatcgctg cgacggcgtt
      901 cagcaacaat aatctgcagc cgttcttttg caatgttggc tgtgtttttc ttccgcgaga
      961 gaaagaaatc gagtaatgcc ataacttatc ctccgaacaa gcgtttgagg aagcctttct
     1021 tctcttcttc aatgaagcgg aaaggacgtt cttctcccaa cagacgttct acggtatctg
     1081 cgtaggcttt acccgcatcg gcgttaatgt cgagaatgac cggttcaccc tggttagagg
     1141 cgcgtaatac tgattgatcc tctgggatca cgccgacgag tttgatgcgc aggatctcca
     1201 gcacatcttc catgctcagc atgtcacctc tgcttacgcg gcctgggtta tagcgcgtta
     1261 acagcaggtg ctctttaata ggctcttcgc cattttctgc gcggcgtgat ttcgacgcca
     1321 gaatgcctaa aatacggtca gagtcgcgta ctgaggagac ttccgggttg gtggtaataa
     1381 tggcttcgtc tgcaaaatag agtgccatta acgcaccggt ttcaatccct gccggggagt
     1441 cacaaacgat aaattcaaaa tccatcgctt tcagatcatc aagaactttg gcgacccctt
     1501 cacgggtgag ggcatcttta tcgcgtgttt gcgatgccgg aagaatatag agattttcag
     1561 tacgcttatc tttaattaac gcctgattta gcgttgcatc gccctgaatg acgttgacga
     1621 aatcgtaaac gacccggcgt tcgcaaccca taatcaggtc gagattacgc aggccgatat
     1681 caaaatctat cacgacagtt ttctttccct tctgggccaa accagtggcg atggccgcgc
     1741 tggaggttgt cttaccaacc ccccctttgc ccgaagtaac aacaataatg cgtgccatag
     1801 aaattccttg ttaaaaaggg atcaatttaa cggttgaacg gtcaaagcgt tttcgactaa
     1861 ctgcagtcgc gccgctttgc cataaaattc tgctgggatt tgatcactca gccagtattc
     1921 acctgcgatg gacaccaatt ccgccatcag gttcgtacaa aatatttgcg tttcccggtc
     1981 accacttgcc cctgccagcg cacgaccgcg catcatgcca tagacatgaa tgttcccatc
     2041 ggcaatcaat tcggccccag cgctaacgtg gcttgtaaca atcagatcac attgtggagc
     2101 ataaatacgc tgaccggaac gcaccggggt atctattaaa cgcgtttttg tgaccggcgt
     2161 ggtattttgc gctggagcct gtggtgcggg agctggacgt ggcgcttttt cctttccttc
     2221 cgtcaggata ggcagcccca ttttttcaat ttcggcttta agttgcgcgt ctttgcagcc
     2281 acttacgcca ataacccgca aaccggttgc cgaaaccgcc ttatgcatcg ctgaccagtt
     2341 taccgggtct tccagtgcac tgacgttgag tacaacgggg gcatgtttta aaaatgcggg
     2401 ggcctgagcg attttgtctt ccagcgcctg atggataacc ttaggttctg cctcatgcag
     2461 atgaaccaca gataaagtga agctactgcc tttaagctcg attggcgtgt ttgacatcct
     2521 ggccttactc aattagctat taatcatcgc cagcgcgcga tgatgttccg aagactataa
     2581 ggcatgttat agtctggatt atattgaggc aagtcaccct cccatttatt cagagtaaaa
     2641 gtctattctg tgataaatgg cgctgattca tagcttaaaa aatacccttg tcaatcaacc
     2701 cattgccgcc gtacttttga ttcttcttat ttacgcttcc tttttccgca ccctaatcgt
     2761 gacagcatct ccagacctgt ttaaatatcc agcattgagg caagtgtatt ttttcaccac
     2821 gccaggcttt cttaactttc cattttttgc agcatcatta aagaacagat acccgttatc
     2881 ttaatatccg ccacagaatt gctctatcat agaaaccgca gaagacgtct gatacttctg
     2941 tcaggtcatc gtaactcgtc caggtcagcg gttttgatta cggattgatg acgaaatgag
     3001 cataagtgtt gtacggttgg attatcagga tgtgctatga atatgatgcg tattatttat
     3061 atcggattgt caggtgtggg gatgatgttc tcatcaatgg cgtctggaca tgacgctggc
     3121 ggacttcaat ctccggcgtg cggcgttgtt tgcgacccgt atatttgtgt gaactcagat
     3181 ggcatttccc cagagttaac aaggaaatat ctcggcgaaa aagccgctga aaacttacaa
     3241 tcattacaag gctacgatcc cagcgaattt acattcgcta acggtgtatt ttgcgatgtt
     3301 aaagaaaaat tatgtcgtga tgatcgttat tttggtgtag atggtaagcg gagtggaaaa
     3361 atcaatcaaa ccaccacaaa gatgttattt atgtgtcgtg agtgatgcct tgtttacatt
     3421 atgttctctg ctattcgtca ttaacctctt gagcgacaga gaggagtggg gcgttaacgt
     3481 ctatgatggg gatagtcccc gtcggttgac atttacttaa tttgaatatc gacgttatat
     3541 tttttagttt tgtttttacg ggcgtcgttt cgagtttgca gcactcgcag ctcatatttg
     3601 ccagaagcag ggagtgagta ttgtccgtga ctatcgagtt ctggggaata tctggataaa
     3661 tcaacagaat catcgattcc tggcccgaag agataagtat cagcgccttc attagagata
     3721 ctcacatgca ctttctgacc ttttttggca tagaaggtat atgtatcgta atcgtatccc
     3781 tttatttcgc cggagtactg agcgctgcta tggcctttgc tgaactcaac attgacattt
     3841 ttacccgcag caaagctcgt ggaggttagc agtgcaagca gcaatacagc cttactgatg
     3901 ctcttgattt tcattggtag tctcctgttc agaatgagta cgatgaactg ttataatata
     3961 acaatccctt acgggagaat tccagtggct tttctgcgtt cggtaaaatc ccggcgcggc
     4021 attttttgcc ggggggatta cttataaagg ggcatggact caacaaaccg cagaaatcta
     4081 cagctggttg gcttgtggcg cgggtttcat tgatgttagc ttatgccaaa acctggcatc
     4141 ttaaagagta aaagtatgtt ttgtgtgatt tatcgaagca gcaagcgtga ccagacctat
     4201 ttatatgtcg aaaaaaaaga cgatttttcg cgtgttcctg aggaactgat gaaaggtttt
     4261 ggtcagcctc agttagcgat gattctgccg ttggatgggc ggaagaaact ggtcaatgct
     4321 gatattgaaa aggttaaact ggcattaacc gagcaaggtt actatttgca gttaccgcca
     4381 ccacctgaag atttgctgaa gcaacatctt tccgtcatgg ggcagaaaac agacgacact
     4441 aacaaataac cgatatccgg cggtggcatt atctttgtcg gcgcgggttt tcatatccac
     4501 gataaggtga ggggaacgtt atgtatcaac atcacaactg gcaaggtgcg ctgctggatt
     4561 atccggtgag taaagtagtc tgtgttggca gtaactatgc caaacatatt aaagagatgg
     4621 gcagcgcagt gcccgaagag ccagtgctgt ttattaaacc agaaacggca ctgtgcgatc
     4681 tgcggcagcc attggcgatc ccatccgatt tcggttcagt tcatcatgaa gtcgaactgg
     4741 cggtgttgat tggcgcgacg ctgcgtcagg caacggaaga gcatgtccgc aaagccattg
     4801 ccggttacgg cgtggcgctt gatctgacat tgcgtgatgt tcagggaaaa atgaagaaag
     4861 ccgggcagcc gtgggaaaag gctaaagcgt ttgataactc ttgtccgctt tccgggttta
     4921 tctccgcggc ggaatttacc ggcgatccac aaaatacaac gctgggcctg agcgtaaacg
     4981 gcgaacaacg ccagcaaggt acgactgcgg acatgatcca taaaatcgtt ccgttgatcg
     5041 cttatatgag caagtttttt accctcaagg ccggtgacgt tgtgttgaca ggcacgcctg
     5101 atggcgtcgg cccgttgcaa agcggtgatg agctgacagt cactttcgat gggcattctt
     5161 tgacaactcg cgttttgtaa tactttttgc cgcctgaaag cggcggcaaa acttgcatcg
     5221 ctgtgccaga ctggttataa ggtgcgtttt aacgtaatgg cggaacacct gatgagcgat
     5281 gtacctttct ggcaaagtaa aaccctggac gaaatgagcg atgcggaatg ggagtcgttg
     5341 tgtgatggtt gcggtcagtg ttgcctgcat aaactgatgg atgaagacac cgacgaaatc
     5401 tacttcacta acgtcgcctg tcgccagctc aatattaaaa cttgtcaatg tcggaactac
     5461 gaacgtcgtt tcgagtttga acccgactgc attaaattaa cccgtgaaaa tctgccaaca
     5521 ttcgaatggc tgccaatgat ctgcgcttat cgtttgctgg cggaaggtaa agatttacct
     5581 gcgtggcatc cgctacttac tggttcgaaa gcggcaatgc atggtgaacg tatctctgtg
     5641 cgtcatatcg cagtgaaaga atcagaagtg attgactggc aggatcatat cttaaataaa
     5701 cctgactggg cgcagtgata aatttaatag atgttgatgg gggctttctt aatatacgtc
     5761 tggatgctcg ccatttcgta gccctgtttt tgaaagcgct ctgtcataat gagagttacc
     5821 cgatacctta ttatcaacag gtaactctca atctttaaaa aacgtctgtg tttttaaatg
     5881 tgataaacct tataccttga gtacatggag agaataatcg ctgatcagac ttcaggtacc
     5941 tcaaagagtg tctttttacc gtgttttttc tgatactcat tacaggtgtt aatcattttt
     6001 ttggccgctt cttttagcaa agaaagcatt aaatcatcat aatcaacgta gaatctggtg
     6061 gtttcagttt ccgtttttat ctctccgatg gcggctattt cggtggttaa tttcaatttg
     6121 gcggcatcga tatctttatt cgcttgttta accgtgttag acagggtggt aaagaaactc
     6181 tgcacagatt ttaacttgtt cttcaattct gggatcagtt tcccttcaac tacgcccgca
     6241 gcaatagaat aggaaatgat taaaccaaat ggaccggcga cgacaccggc tgcggcaccg
     6301 gcatacgctt ccttcctgat tttatctacc tgtgactgga aatagctgct tttttctgaa
     6361 aaatcattgg ttaactggct atctaacgcc agcagtttcc cggaagcgtt gttgaaactt
     6421 tgtgagctta ccagcaggga tttttgcgct tcattcagct tcgtgatgcc gtcatccagt
     6481 acct
//
LOCUS       DAEEKQ010000202         6305 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.204, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000202 DAEEKQ010000000
VERSION     DAEEKQ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.204"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(336..4193)
                     /gene="sigA"
                     /locus_tag="KLK57_003755"
     CDS             complement(336..4193)
                     /gene="sigA"
                     /locus_tag="KLK57_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HBE0894014.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFLSSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGELTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASSGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(4554..5558)
                     /locus_tag="KLK57_003756"
                     /pseudo
     CDS             complement(4554..5558)
                     /locus_tag="KLK57_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            5543..5920
                     /locus_tag="KLK57_003757"
                     /pseudo
     CDS             5543..5920
                     /locus_tag="KLK57_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014839879.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(5959..>6305)
                     /locus_tag="KLK57_003758"
     CDS             complement(5959..>6305)
                     /locus_tag="KLK57_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0894015.1"
                     /translation="VPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGAW
                     LIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIAL
                     AAQSSFSQQKQN"
BASE COUNT         1571 a         1408 c         1412 g         1914 t
ORIGIN      
        1 gggagacaat gctgtattct tttttaaagc actcaccaga aacggtgatt gtaatgataa
       61 tggtcttctc gatcgagatg taaatacccg tttcgttata ctgtccgtat cggaaccatg
      121 agaactcagt tgcattccag ccatgctcca gaatatgtgc atttttttgc ctgatagttt
      181 ctttttttat tcgctcatgt ttaaggcata acaggcagat aaataatgtc agaaaaagaa
      241 agctaataat gagagcaaga gcgatatagt ctgtcacagg gtaatacata ttctctacgg
      301 tagaagaagg gccgcaaacg cggcccgggc tgttatcaga aagagtaacg gaagttggcg
      361 ttgatcgcgt tatccacgtt gtatttacca aatgccgatt tctcaaactc aagaccgaag
      421 cgaagattat cgcgaatttc ggcgttgaga ccaacattca tgagaatacg accgtctttt
      481 tcacctttga tacgtttctc accggacgca tcacgcagta cggtttcacc gttggcaaac
      541 aggtcaaact ggtagccaag gccggcgcgg gctgtgactt tccagtcctt accggagaag
      601 gatttaccca catcaacacc ggtacgccca atcagcggat taaagtcctt atccttcatg
      661 gtcacgctca ttccgcgatc ctgccagtcg aaccgtttac ctgatacggc cccataaacc
      721 agttctgcct gcggctcaat ccaggagtct tctgttacat gatagcggta gcctgcttca
      781 gcacccacat acaaggaatg agaactgtag tcttttgttc cgagtccagc aaaggtcgcc
      841 gagtactcat tatcatggtg aacatactta ctaatcaggt cgatataggc accagagtca
      901 aatatagcgg aagcatacag acctgccccg acggacttcg tcttgccact gaatgcatta
      961 ctgctggcat gactgtcggt atacgtcata gtcagaccag tgaaaaggtc aagtccatcc
     1021 agctcatgtt ttttatccac accaatctgc acatgggtgt agttgtcact gtatccacta
     1081 gaagctgaac ctgctccgct catgatgcgg gcccatgcac cggcctcgcc attggtgtca
     1141 cgcagatccc ccattcgttt attcaggttg ttgacttctg tcaggaaggc tttgtaatta
     1201 ccggacatat acgattttgc tgcaccggaa gatgctgtat tttcagccac cttatatccg
     1261 gtcagggtcc acgtggtatg ttcctgatcc tctttcgtgc tgatcacagg agatatgttg
     1321 ctgaaaccta tggcctgagt actggcttta aatatctctt tattgcttcc ttttggggct
     1381 gttatcaggt caacgtttaa tcctgagtta ttatttccca ctttctgtat atcgaccgac
     1441 agactgttat tgcttccggt taactgattt ttaacaatga gtttatccga ttgatttaca
     1501 gactggctca gatcagcccg cataatcaca gcagtattag tggcatccag tgtgtttact
     1561 gtcacagtat ggaaacccga attctctatg ttactaaaca acaaaagact gtcgcgggtt
     1621 tttaatgatt gcagggctga ctgtccgttt actctccata aggtgttata catgctggct
     1681 gaggcagcag gagcatcaat acctccggta taagaagtat caaagccacc cagcaaacct
     1741 gtagccagcg gtgtgtgtgt ggttttgtcc aggttatctt ctttatactg gtcaacacca
     1801 aacagaatag atgctttttt gtctgatttt atattaccat tgacccaggc ataattttga
     1861 gcctttaacg tggcaccatc ttctgtcata ttatatccat ctgacatata gattgccggg
     1921 ctgtatttat tattttctgt aggggtgcct gttaatgtca gggttccggc cagttcaata
     1981 gctttatcta catacatttt tccctgggcg gtaacagtag ctcctttgtt gataatcagg
     2041 tctgtattaa ttaaggataa tgcaccagtc atagtaacag ttgatttatc accaacagtt
     2101 attttgctgt tttgagcact cagactgccg ttgaaactac tttcgcctgc tgaatccccg
     2161 gagcgaagct gttgtctgaa tccaaaacct tcgccagtaa tgtttcttcc tgagaacatg
     2221 tcaatgtatg cagttttatt tcccagaaca atttgagagt ttttagcctc tatgtgtcct
     2281 tttacattag catcccggcc tatactcagg gttgcgtttt ctaaatttaa tttccggaaa
     2341 ttaaatttac gactttccca gtcgggctgg gaaaaagagg ctatctgatt gttggactta
     2401 tattccgtat tgtaacgttt atttacagtg ctttcctggt cctttatggc agcagaatag
     2461 tctttttgac ataaaaaagg aattggacag ttgttattgc cttctttaaa tatggcatgt
     2521 tctgtcgcat gtccttgtaa tgttaactgt gcattccgga cagagatatc gtttttgata
     2581 tcgactgagc catcaaagat cagattggca ttattgttat gttgctgggt agtattgata
     2641 atatgattta tgcttatatt acctgataca ttcccatggt acatatatgc atcagtatta
     2701 gtaagtgata atgttgattg cttcacgttt gagttcgtta cggttgttcc cgcatctgtt
     2761 gctgcgattt tctgaaatga ctggtcatag ccgtttagat ccagtgttcc gccatttctg
     2821 gtaaaataga tttcgccatt accgctttcg cttaaagcgt ctttggcatt tatttttacc
     2881 gtacctttac ctccggccat gtaaatttta ttgaaggctt tttcagcact aaggatgaca
     2941 gtcccattac ctattttaag gttatttccc tgtgctattt ttacatccag agtaccacta
     3001 cctattttat gcagattatc tccggcaacg cccttaacat tccattcaac ggtggtatta
     3061 ttatcaatat ctatcccggc acctttataa gacttatctt ttccgtacac tttatatgtc
     3121 tggttcttat cgaatataag cccgccaatg ccaagatcgg tatcctgctt gagcgttaat
     3181 tcccctcctc ctgagacaga caggtcctta tcttttataa tcgatgataa ctcttgtttt
     3241 ttaccatcca gctgcatagt gtttgtgaaa ttctttttta aattgtttac cagaatatta
     3301 ttaaataacg agtagtttgt ccaggctata ttggtactac tgagaaaacc aactgtactg
     3361 gtaacaccat ggaaaaccca tttttgttgt tctttatcat atagataaga accagaacca
     3421 ctatcgccaa aaattggtga gtttttaaaa ggggttctgt tggtataaat gtaactcttg
     3481 tttttccagt ccagattaaa aagacttgcg cttaacaagt cgggattata gttttgtcca
     3541 aacagatatt gtttgccgtt taatatcgtt gaggttgttc cggcacctgc tctgaagcca
     3601 attatttgtt ccttaccgtt gtagtttacg ccatatcttt tcatcgcatc ttcgggagaa
     3661 agtgagaagt cggcactctc cgtaacccct gttgtttcca cgacgaattt attcagacga
     3721 tgaaccgaaa agtcgccaga tgatactctg ttacttgctt tatatgctgt ctgatcccat
     3781 gactgtgttg ttattgcatg atgctgtgta ccgttatgag tcgcagtaac actgtacgca
     3841 cctccaattg atgttgttgc acctttgttg gaaacagcag aaaaatcagg tacaggtagt
     3901 tctggaaaat gaaatatctt tccatctttc atcattaatt gaacatttgt tgcacccggt
     3961 ctgaactctc ctctgttttg agcaaggtca agataatcgc gggtccatat attatttata
     4021 tccagcattg ctgcaaatgc tgtttcgcca taacttccat atattgagga taaaataata
     4081 ccgaggataa ctttctttct tgatgtaccg acactaactt tccgggtcag ttcagaaaca
     4141 gcaactaatc cacctgtaat atgactatat ttcagtgaat aaattttatt catacgtgaa
     4201 ctccgatatt tctgagttgt ggttatggga tagcatttaa ttacaacggg gaactatcag
     4261 tcatggtacg taactgataa gggtataatg ataatcactg tcagttatat ttgtatgaca
     4321 gatgtaacaa aatgaaaaaa aagataagca caagtgattg attggtcaga tattgatcac
     4381 ttggtgtttg tattttgaac tttgcgatca aaatttgatc tttgttgttt ccggtcaatc
     4441 ctttacaggt tttctgtatt atccggggga gttaagtctg tcgatgtccg acagactttt
     4501 gatgatggaa acaacatgca gcgataatgt gcatgctgtt ttgcaggtgg aactcagaag
     4561 gtgaactgga actgtgcgac cgcaccgtaa gtgttgtccg tgccgatcag gcctgcaaca
     4621 tcaaggtgaa ccacatcaaa cggtgagatc cccaccccgg ccgtgaatgc actgccgtcg
     4681 ttaccggcca gattctgacg gtaaccggcg cgcagctgtg cccatttcca ggcattaaat
     4741 tctgcgccaa tggcggcaaa ctgacggttg ctgtcggagg tgaaaccgct ggccggggtc
     4801 agatccacat caaatgcggt ggtgaacatc gcgttgtgcc aggagacacc ggccgtcacc
     4861 tgcgggcgga tcctgaaggt ttccgtgata ccgttcactt cttttgtctc aatactacgg
     4921 gggatcaggt tctgtgcgac cagtcccagc gtccagttgt catccaggtc cgtactggca
     4981 ccgatgtcgg cgttgatgcc atttttcgtg ttgtggtagc ggtcgccttt gaaggcgctg
     5041 ctgtcataat ttctgaccag cacgttgtag ttgaacaggt cgacgcgctg gaatttgggg
     5101 gggaagccca gagaccattt ctgcccggcc gtttccagtt ccgtagccag agcaataccc
     5161 acatcagtga tcagtgccgc gcgggcaaag gcctgtgatg tcaggcggct tttgtccacc
     5221 tcctgcccgg tgctgtttgc cacctcttcc aggtaagtga ggtcagcatc agagacttta
     5281 ccgtcgacac tgacggtgcc gtgtgattta accatcagtg cggcagagag gcgatcaccg
     5341 ggaagggccg ccacagcaga aactcccagc tgtgcggcgg catgtgtgtt acggaagtcc
     5401 cggaggtgtt ctttcaggcg tgcggcagcc tcaggaacac cgtggttact ttcgatggcg
     5461 cggtcgaagg ccttccactc atcactgatc cggtcggcgt tgtcggtgat attatccgga
     5521 ttggagagct gcgcgccgac ggacggaagc aggatgctgg gctcatgcgc gacgcaaaat
     5581 ccacgatgta tatatcagta ctaaaagcgc gacggcggaa gaagctctga aactaatcgg
     5641 tgaactgtac gccatagagc acgatatacg cgggttaccg gtgtctgaac gcctggcggt
     5701 caggcaaatg cagagtagac cgctcctgac ttccctgtat aagctgatgc aggagaaaga
     5761 acacacgtta tcgaagaaat gtcgtctggg agatgcgttc cggtatatca ggaagcactg
     5821 ggctgcgttg tgcaacttct gtgatgatgg cctggctgag gtagataata atgcagcaga
     5881 aagagcgctt cgtgcagtct gtctcgggaa gaaaactttt tcgaggatca ctcattccgt
     5941 gtctgactgc ttcagggttt aattttgctt ctgctgactg aatgatgatt gggcagcaag
     6001 tgcaatatcc cacataaatc cagttaactc acgagccacc gccgtaatgg ctacttgtga
     6061 ttttttccct tttgctgtca gagttcggta acgagaacac aatcgttgtt gagctttcca
     6121 tgcaatatct ttggaatatt gtgttacaga gtccggtcgg taatatataa gccatgctcc
     6181 aacttttgca ggtgtacgat aagaccaagc ggcttcgtaa agcagacgtc tcagttcgct
     6241 gtttccaact tttgttattc ctctgggacg aatactgttt ccactggaat attcacctgg
     6301 cacca
//
LOCUS       DAEEKQ010000203         6305 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.205, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000203 DAEEKQ010000000
VERSION     DAEEKQ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.205"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            191..2038
                     /gene="ldtD"
                     /locus_tag="KLK57_003759"
     CDS             191..2038
                     /gene="ldtD"
                     /locus_tag="KLK57_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HBE0894016.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAVFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2219..2767
                     /locus_tag="KLK57_003760"
     CDS             2219..2767
                     /locus_tag="KLK57_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HBE0894017.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2794..3441
                     /gene="gloC"
                     /locus_tag="KLK57_003761"
     CDS             2794..3441
                     /gene="gloC"
                     /locus_tag="KLK57_003761"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HBE0894018.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="KLK57_003762"
     CDS             complement(3663..4853)
                     /gene="aspC"
                     /locus_tag="KLK57_003762"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HBE0894019.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSSLINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="KLK57_003763"
     CDS             complement(5038..6126)
                     /gene="ompF"
                     /locus_tag="KLK57_003763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HBE0894020.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVELVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         1570 a         1554 c         1604 g         1577 t
ORIGIN      
        1 ttacggcgcg cttttcttta aacttcttta cattaggtta tgtaaaaacg tatcggcgtt
       61 tatatactga agataagcct gatgagtaac aggcttgctc gtcatacttt cgtgagtatt
      121 ggcgttgtac aggcaagtcg taaaataaca gcctggctat tcagagtatg ataaaaacag
      181 ggggcaaggg atgttgctta atatgatgtg tggtcgtcgg ctgtcggcaa tcagtttgtg
      241 cctggccgta acattcgctc cactgttcaa tgcgcaggcc gatgagcctg aagtaatccc
      301 tggcgacagc ccggtggctg tcagtgaaca gggcgaggca ctgccgcagg cgcaagccac
      361 ggcaataatg gcggggatcc agccattgcc tgaaggtgcg gcagaaaaag cccgcacgca
      421 aatcgaatct caattacccg caggttacaa gccggtttat cttaaccagc ttcaactgtt
      481 gtatgccgca cgcgatatgc aacccatgtg ggaaaaccgt gatgctgtta aagccttcca
      541 gcaacagctg gcagaggtgg cgattgccgt tttccagccg cagtttaata aatgggtaga
      601 gttactgacc gatcctggtg ttaacgggat ggcacgcgac gtagtgctct ctgatgcgat
      661 gatgggctat ctccatttca ttgcaaatat tccggtcaaa ggcactcgct ggctatatag
      721 cagtaaacct tatgcacttg caacgccgcc gctctcggtg attaaccaat ggcagctggc
      781 gctggataaa ggtcaattgc ctacgtttgt tgcaggactg gcaccgcagc atccgcaata
      841 tgcggcgatg catgaatcgt tactggcctt actcagtgac accaaaccgt ggccccaact
      901 gaccggcaaa gcaacgttgc gcccagggca gtggagtaac gacgtaccgg cgttgcgcga
      961 aatattgcaa cgcacaggca tgttggacgg ggggccgaaa attactctac ctggcgatga
     1021 cacgccaact gacgcggtag tcagcccatc cgctgttact gttgaaacag cagaaactaa
     1081 gccgatggac aagcaaacga cgtctcgtag taaacctgcg cctgccgttc gcgccgccta
     1141 cgataatgaa ctggtggaag ccgttaaacg ttttcaggca tggcaaggat tgggggcaga
     1201 tggtgctatt ggcccggcaa cacgtgactg gttaaacgta acgcccgccc agcgtgctgg
     1261 cgtgttggct ctcaacatcc agcgattgcg cttgctgcca acagagcttt ctaccgggat
     1321 catggttaac attccggcct attcgctggt ctactatcag aacggcaatc aggtgctgga
     1381 ttcgcgagtc attgtcggtc gccccgatcg caaaacgccg atgatgagca gtgcccttaa
     1441 caacgtagtg gtaaacccgc cgtggaacgt acctccaact ctggcacgca aagatattct
     1501 gccaaaagtg cgcaacgatc cgggatatct cgaaagccat ggctatacgg tgatgcgcgg
     1561 ctggaacagc agagaagcga ttgacccatg gcaggttgac tggtctacaa tcacggcctc
     1621 gaatttaccg ttccgcttcc agcaggctcc aggcccacgg aactcgctgg ggcgctataa
     1681 attcaatatg ccgagttcag aggccattta tttgcatgac acgccgaacc acaatctgtt
     1741 caagcgtgat acacgcgcat tgagctcagg ctgtgtacga gtgaataaag cttccgatct
     1801 ggcgaatatg ctgttgcagg atgcaggctg gaatgacaaa cgtatttctg atgcgctgaa
     1861 gcagggtgat acacgttacg tcaatattcg gcagtcgatt ccggtgaatc tctactacct
     1921 gacggccttt gttggtgcag atggtcgtac ccagtatcgt acagatattt acaattatga
     1981 tctgcctgcg cgatccagct cgcaaatcgt atcgaaagcg gaacaattaa tcaggtaaat
     2041 gaagtagttc ggggaaatca gttgtcgtaa taattagcat gattgggggc gattctctgc
     2101 agcccccgtc actgctgggg ttgagtcatc ttgacgtctg ctttacgggc ggttaaggtg
     2161 cctcttgtgc gccagaagtg catataaacg ataacattga cctgtagact tgattatcat
     2221 ggacaaattc gacgctaatc gccgcaaatt gctggcgctt ggtggcgttg cactcggtgc
     2281 cgccatcctg ccgacccctg cgtttgcaac actctctacc ccacgcccgc gcattttgac
     2341 actcaataat cttcataccg gagagtcaat caaagcggag tttttcgatg gcagaggcta
     2401 tattcaggaa gaattggcaa aacttaacca ttttttccgc gattaccgcg cgaacaaaat
     2461 aaagtccatc gacccaggat tattcgacca gttgtatcgc ctgcaagggt tgttaggcac
     2521 gcgcaaaccg gtgcaactca tttccggtta tcgttctatt gataccaaca atgaactacg
     2581 cgcccgcagc cgtggagtag cgaagaaaag ctatcacact aaaggccagg cgatggattt
     2641 ccatattgaa ggtatcgcgt taagcaatat tcgcaaagcc gcgttatcta tgcgcgcagg
     2701 tggtgtagga tattatccac gtagtaactt tgtgcatatt gataccgggc cagcacggca
     2761 ctggtagtaa ttgcttaacg aaacaggggc agtatgaact atcgtattat tccggtcacc
     2821 gcattctccc agaactgttc attaatctgg tgtgaacaaa cccgtctggc cgcactggtc
     2881 gatcctggcg gcgatgcgga aaaaatcaaa caggaagttg atgacagcgg cctgacactg
     2941 atgcagatcc tgctgacgca tggtcatctg gaccacgttg gcgcagcggc ggaactggcg
     3001 caacattacg gcgtgccggt ttttggcccg gaaaaagaag atgagttctg gctgcaaggc
     3061 ttgcctgcgc aaagtcgtat gtttggtctg gaagagtgcc agccgctgac gccagatcgt
     3121 tggctgaacg aaggcgatac catcagcata gggaatgtga ctttacaggt gttacattgc
     3181 cctgggcata cgccgggtca tgtcgtgttt tttgatgatc gggcaaagct gctgatttct
     3241 ggcgatgtta ttttcaaagg cggagtaggg cgcagtgact tcccgcgtgg cgatcataat
     3301 caactgattt cttcaatcaa agataaattg ctgccactgg gggatgacgt gacatttatt
     3361 ccgggtcacg gaccattatc cacacttggt tatgaacgcc tgcataatcc cttcctgcaa
     3421 gacgaaatgc ccgtctggta aggcacataa aaaagcccgc ttttaatgcg ggcctggatt
     3481 tctggcaaag tgcgctttgt ttatgccgga tgcggcacga gcgccttatc cggcctacaa
     3541 aatcgtgcaa attcaaaata ttgcagggga cgcgtaggcc tgataagcgt agcgcatcag
     3601 gcaatgttgc gtttgtcatc agtctcagcc cgcttttcag cgggcttcat tgtttttaat
     3661 gcttacagca ctgccacaat cgcttcgcac agcggagcca tgttatctgg tgtcatcccg
     3721 gccacattta cgcgaccaga agcaaccgca tatacgccaa actcttcgcg cagacgcagc
     3781 acttgttctt ttgtcaggcc actgaaggag aacatgccgt tctgtttgat gataaagctg
     3841 aagtcgcggt ttgcgccttt ttcctgcagc gtattgacga acaactgacg catacgctga
     3901 atacgctggc gcatatcagt cagctcttgt tcccaaatcg cacgtaacgc atcgttgctc
     3961 aggatggtgg caacaacaga agcgccgtgt gctggtgggt tagagtagtt agcgcgaatc
     4021 gccgctttca tttggctgaa tgcgcgatca acggtttcac tgtcggcagc aaccagagta
     4081 caagcgccaa cacgctcgtt gtacaggcca aagtttttag agtaggaact ggcaacaatc
     4141 agctctttat gcatagccgc gaaagcgcgc agtccttcag catcttcttc cagaccacgg
     4201 gcaaaaccct ggtaagcgaa gtcaaacagc ggtaaccagc ctttctcaac ggagagttgt
     4261 gccagtgtct gccattgttc cagcgtaggg tcgataccgg ttgggttatg gcagcagcca
     4321 tggaacagca ctacgtcgcc agcctgagct tcattcaggc tgttaatcag tgcatcgaag
     4381 tcaagagtgt gattttccgc atcataataa gcgtattcac gaacttccag acctgcagag
     4441 ttaaagacgc tcttatggtt cggccagctt gggttgctca cccacactcg cttaacgctg
     4501 gtattttttg ccaggaaatc ggcagctacg cgtagtgcgc cagtgccccc cggagtctgt
     4561 gccgtgcgag cacgtttgtc attgatcagg gagctacctt taccaaacag cagttcctga
     4621 gtgcagcgac caaattcagg gatgccgtca atgccgaggt aatttttggt ggtttcattt
     4681 tcgagcagat actgttcagc ctttttcacg ctggtcagta ccggggtttt gcccgtctca
     4741 tctttataga caccaatccc gaggttaatt ttgccgggac gttcatcggc acgaaacaga
     4801 tcggccaggc ccagaatcgg gtcggcagga gcggcggtaa tgttctcaaa catgacgagg
     4861 ttccattatg gttacagaag ggaagtccgc tatcagggta acgggagatt tacaaaattc
     4921 caactattac tgatgaaaac gcaggctgtt tttgcaagac gtgagattgc tctggaaggt
     4981 ataaaaaaaa caggaccaaa gtcctgtttt ttcggcattt aacaaagagg tgtgctatta
     5041 gaactggtaa acgataccca cagcaacggt gtcgtctgaa cctacgccca gtttgttgtc
     5101 agaatcgatc tggttgatga tgtagtcaac ataggtggac atgtttttgt tgaagtagta
     5161 ggttgcgccc acttcaaagt agttcaccag ctcaacatca ccgatacctt ctacgtcttt
     5221 cgctttagat ttggtgtaag cgatggacgg acgcagaccg aaatcgaact ggtattgcgc
     5281 aactaacaga acgtcttgcg ttttgttggc gaagccgctg gtgtttgtaa atttattagt
     5341 gatcggcgta gcgttacggg tttcaccgta gttcgctgcc aggtagatgt tgttcgcgtc
     5401 gtacttcaga ccagtagccc actgttcagc ttttttaccg ttgccaagag gttgagcttc
     5461 ttgcaggttg gtacggtcag ctgcaccata agcaccaacg ataccaaagc cttcgtattc
     5521 gtagctgata gaaccgccaa caccgtcgcc gttagaacgg cgtgcagtgt cacgctcgtt
     5581 tttacccagg tactgaacag cgaagttcag gccatcaacc agaccaaaga agttggagtt
     5641 acgataggta gcaacgccgc caacacgacc aacgaagaag tcatcgctgt atgcagtatc
     5701 accaccaaat tctggcagca tatcggtgta acccagtgca tcataaacca caccgtagtt
     5761 acggccgtaa tcgaaagaac caacgtcagc gtatttaaga cccgcgaatg ccagacgcgt
     5821 tttgttacca gtttgagcgt cagcgccttc agagttgtta ccctggaagt tatattccca
     5881 ctgaccataa ccggtcagat cggaattgat ttgagtttcc cctttaaaac caagacgggc
     5941 ataggtcatg tcgccattgc caccgtaact gttttcaccg ttacccttgg aaaaataatg
     6001 cagaccaaca gctttaccgt acagatctac tttgttgcca tctttgttat agatttctgc
     6061 agcgtttgca gtacctgcta ctaacagagc agggacgatc actgccagaa tattgcgctt
     6121 catcattatt tattaccctc atggtttttt ttatgacacc tgccactgcc gtcaataagt
     6181 tctgtcaata aaaatttacg gaactattga tgagagtttg gtgtctttat gtgtctgcag
     6241 gcatctttcc attcaaacta acgtttcgct accgtgaaag tgctacaaag ataaagattt
     6301 agttt
//
LOCUS       DAEEKQ010000204         6254 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.206, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000204 DAEEKQ010000000
VERSION     DAEEKQ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.206"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            123..953
                     /locus_tag="KLK57_003764"
     CDS             123..953
                     /locus_tag="KLK57_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418745.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfZ/YjiC family protein"
                     /protein_id="HBE0894021.1"
                     /translation="MSMPLSNALQSQIITDNHFLHHPIVDSEFTHKLKYARMDLENIY
                     LPPLTRGNNHNYDGKSAVEIRKLDISKESWPFNYVTGACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIMTVTGVANSSGFALAALLNANIELGN
                     DPIIGIEAYPGTAEIHAKMGYKVIPGDENAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1094..1867)
                     /gene="uxuR"
                     /locus_tag="KLK57_003765"
     CDS             complement(1094..1867)
                     /gene="uxuR"
                     /locus_tag="KLK57_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HBE0894022.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWRHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2082..3542)
                     /locus_tag="KLK57_003766"
     CDS             complement(2082..3542)
                     /locus_tag="KLK57_003766"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HBE0894023.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3623..4807)
                     /gene="uxuA"
                     /locus_tag="KLK57_003767"
     CDS             complement(3623..4807)
                     /gene="uxuA"
                     /locus_tag="KLK57_003767"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HBE0894024.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLVQCGICTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5147..>6254
                     /gene="gntP"
                     /locus_tag="KLK57_003768"
     CDS             5147..>6254
                     /gene="gntP"
                     /locus_tag="KLK57_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HBE0894025.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIIS"
BASE COUNT         1481 a         1619 c         1586 g         1568 t
ORIGIN      
        1 cgtgtagagt tgatatcact acaacagaaa catgcatgtc atttgtttac ttctcttttt
       61 actacagaaa aatttatcta tttttctaaa catcagtgaa atactcataa aaggtaacta
      121 ttatgtctat gccattaagc aatgcccttc agagtcaaat tattacagat aatcacttcc
      181 ttcatcaccc aatagtcgac agtgagttta cacataaatt aaaatatgca cgcatggatt
      241 tggaaaacat ctatcttccg cctcttactc gtggcaataa ccataattac gatggaaaaa
      301 gcgctgtcga aattagaaaa cttgatatta gtaaagagtc ctggccattt aattatgtga
      361 caggagcatg cagagaatcc gatggtatta caacaacagg acgtatgctc tatcggaact
      421 tgaaaatcac ctcagcttta gacgaaatat atggtggcat atgtaaaaag gcgcatgctg
      481 ccacagagtt agcagaaggt ttacggctaa atttatttat gaagagccct ttcgatcctg
      541 ttgaggacta tacagttcat gaaataacat taggccctgg ttgcaatgtt cctggttatg
      601 ccggaaccac gataggatat atttcaaccc ttccagcttc tcaggcaaaa agatggacca
      661 atgagcagcc aaggattgat atctatattg atcagataat gacggtaaca ggggtagcaa
      721 attcttccgg attcgccctt gccgcattac ttaatgctaa tattgagttg ggtaatgacc
      781 caataattgg tatagaagcc tatcccggta ccgcggagat ccatgcaaaa atgggatata
      841 aagttatccc gggtgatgaa aatgcaccac tcaaacgtat gactctgcaa ccgtcatctc
      901 ttccagaact gtttgaatta aaaaatggtg agtggaatta tattggtaaa taactaccag
      961 gaagaatgag tactaacatt caggttatac agatagctaa acaataccct gcaatttaca
     1021 aagtaatact cattgcgact tcttaattta agaaagcgca aggaacattt acccttgcgc
     1081 ttattataat aagtcaggcg tcaactttat ccagcggcca tgaatcaaac agatagccat
     1141 caaaataaat atcgtcaacg ttcgagaatt ccagcagacg ttgcttaacg ttttccagat
     1201 gccgccacat tgccagcttc gccgctcggg catctttttt gattaacgcg gcgaggatct
     1261 gtttgtgatc gcccaaccac tctttgcgat acaggctgtc atccagatgg ctgtgcaact
     1321 ggatccacat tggattgttt tcccgccact gccaggactg acggaacagc tccaccagca
     1381 tgctgttatg cgttgcttcg gcaatggcga gatggaactg catatcacca ctttcgctgc
     1441 tgcccggcgc gctggaagcc agctcacgct cttccagttg caatgcctga cgcattttga
     1501 cgatatcttc gcgggtagcc tgcaaagcgg caaattccgc gatgttactt tccagtaact
     1561 gacgcgcctg taacagctca aaaggaccgg catcattgca gacgttagca tccggactgt
     1621 ctgtgttgtg gctgcctgag ctatcaagaa catagatacc ggcaccccgg cgcacttcca
     1681 ccagcccttt gatctccagc atgatcagcg cttcacgtac caccgtcctc gtgacatcaa
     1741 gcatttctgc aatttcacgc tccggcggca gccgttcgcc aggattgtat ggcgtcttta
     1801 tgatcagatc gcggatcatc gccccgactt cctggtaagg tctttgcgca gaggtggcag
     1861 atttcatggt gttgtgtccg ttaggtgcgg ttaatcaaat ttttgccgga tgcggcgtga
     1921 acgccttatc cggcctacaa cgggcaattt tacggcaccc gttaatctca aactatacca
     1981 ttagcactgg aagttggcac gtacaggtgg gcaaaaaaac gaaaactggt caaccaaata
     2041 tcagtttgat tgaccagtaa gtctgtaggg taatcagtta gttacagcgc agccacacac
     2101 tcgcgcgcac cgcgttcgca cagctgctga tatgccgccg tcaccgcgcc aacaaagtcg
     2161 gcattctgcg gcagatcatc ggcaaaaatg ccgctcaggc ccagcagcgc tttcacgcgg
     2221 tctgcgccct gatactgcgc gttaatcttc tggaactcgg ccagcatcgg gtcaaccacg
     2281 tcaatggcat taccctgttc atccacgccc tgggtgtagc gcatccagcc agccacgccc
     2341 agtgccaggt gacgccagct gccgccgttt tgcaggtgca gacgcaccgg atccagaaga
     2401 cgctgcggta acttctggct gccgtccatt gcaatctgcc aggtacggtg acgcagagac
     2461 gggttgctga aacgctcgat cagcagcgtc gcataggcgt tcaggtctgt accttccggc
     2521 atcgacagcg ttggcgcttg ttcctgcatc atcagggcaa aggccgcttt gcgataatcc
     2581 ggattagtca tggtgtcggc aatggtttca tagccgccga ggtaaccgag gtacgccagg
     2641 aaagagtggc tgccgttcag catacgcagt ttcatcattt cgaacggcac aacgtctgca
     2701 acgaactgtg cgcccacttt atcccagtcc ggacgaccat taacgaagtt atcttcaatc
     2761 acccactgac ggaacggttc gcaggcaatg gcgcacgggt cgtaaacacc cagctggtca
     2821 gcaatttcct gtaaggtttc tggcgtcgcc gccggaacga tgcggtcaac catggtgcac
     2881 gggaaggtga cgttctcttc aatccatgcc gccagctgcg gatcacgtgc ctgagccagc
     2941 cccagaaccg cgaccttcgc cacatgaccg ttttcacgca cgttgtcgca ggacatcacc
     3001 gtaaacgctt tcagcccttt ttcacgacgc agacgcaagg cttcgacgat gtaaccaatc
     3061 gcggacttcg gcgcagtcgg gttttccaga tcgtgcttga tcagcgggtt attgagatcc
     3121 agctgaccgc ttgccgcatc ggcgcaataa cctttctctg tgaccgtcag ggagacaatc
     3181 gccgtctgcg gacgcgccat cgcgttgaga ataccttcac aaccgtcgat ttccggatgc
     3241 agggcttctt tcatcgaacc gataattttc agctcggtgc tttctgcgcc tttttccgcc
     3301 acggtgtaca gcagttgctg ttttttcagg ttttcaatca gcacacggtc gttgcctggc
     3361 atcaggttaa cttcgcagat accccagtcg ctgtcggtac tttccagcag atgatgggtg
     3421 tacagcgcct ggtgcgcgcg gtgaaacgcc ccgcaaccga gatgcacaat gcgtgattcc
     3481 agacgagaat gatcccatga cgggcgggca accggcagat tgctgtcaac aatagtagtc
     3541 atcgtaaact ccagaatccg tcttcagaga gtgacggcaa ggaagaagga accggcacgg
     3601 cgcgcagcca tgccggtggg tatcaacggc taaagaaagc gcgctggatc gccagttcga
     3661 caccgcgaac ttcagccagg cctttcagac gaccaattgc ggagtaacct gggttggttt
     3721 tcttcttcag gtcgtccagc atctgatgac cgtggtccgg acgcatcggg atcaggtctt
     3781 ctttgccttc cgctttacga cggtgttctt cttcaacaat cgctttcacc acttcgtaca
     3841 tatcaacgtc accgttcagg tgcgccgctt cgtggaaggt tttcgggtta tcttcacgca
     3901 tggtggagcg cagatgggtg aagtaaatac gcggaccgaa ctgcttgatc atatcaacca
     3961 gatcgttgtc agcacgcacg ccgtaggaac ctgtgcacat ggtgaaaccg ttcgccatgc
     4021 tgtttacggt atcaaccatc cactgcatat cttcaatggt ggaaacaatg cgcggcaggc
     4081 cgaggatcgg gcgcggcgga tcgtccgggt gaacagccat acggacgcca acttcttcgg
     4141 caaccggaat aatcgctttc aggaagacgg caaagttttc gcgcagtttg gctttgtcga
     4201 tatctttata cagctccagg tgcttacgga actggtcgag ggtataccct tcttccgcgc
     4261 ccggcagacc ggcaatgatg ttacgggtca gacgcgcttt gtcttcgtcg ctcatagtgg
     4321 cgaagcgttc agcggcctga gcaatttctt cttcggtgta atccgcttcc gcgcctggac
     4381 gcttcaggat atgcatttcg aatgcagcga attcgatctg gtcgaagcgc agagctttgg
     4441 agccgtctgg cagcacgtat tcgaggtcag tacgggtcca gtcgagcacc ggcatgaagt
     4501 tgtagcacac ggtgcaaatg ccgcactgca ccaggttgcg cagagtctgc tgatagttag
     4561 cgatccactg ttcatagttg ccagtgtggg ttttgatatc ttcgtgaatt ggcacgcttt
     4621 ctacgacaga ccataccagg cctgcgtctt caacgatcgc cttgcgtttg aggatctctt
     4681 ctacggacca tacttcgccg ttcgggatat ggtgcagcgc ggtaaccacg ccagttgcgc
     4741 ccgcctgacg gacatcagct agagaaaccg gatcgtttgg gccgtaccag cgccaggtct
     4801 gttccatgat ttttcctctt tcacatagtg ggttccacag caaagagacg tggagccccg
     4861 gagttgttta ggtatccgga tgaacaacca gaccgcagcc gtggcgttgc atcggatgag
     4921 atggcgtata agttctacct tttggtaaga catgtcaacc cgaaatctga aattggttaa
     4981 ccacatcgca agaatttcac tctcacggtt gttgctgcaa attgcagcaa agggtaatcg
     5041 atggttattg atcagaggat gtgacattca tcgcaacaat ggttgaccaa tttacataac
     5101 atgtcgccca aaataacaac ggttcaacca ggtaaggtaa aacgttatgc atgtgcttaa
     5161 cattctctgg gtggtattcg gcattggcct gatgctggta ctgaatttga agttcaaaat
     5221 caattcaatg gtggctttgc tagtggcggc gctgtccgtc gggatgctgg cgggcatgga
     5281 tttgatgtcg ctgctgcaca caatgaaagc gggcttcggc aacacgctgg gggaactggc
     5341 tatcatcgtg gtgttcggtg cggtcatcgg taaattgatg gtcgactccg gcgcggcgca
     5401 ccagatagcg catacgctgc tggcgcgtct cggtctgcgc tatgtacagc tgtcggtaat
     5461 tatcatcggc ctgattttcg gtctggcgat gttctatgaa gtggccttta tcatgttagc
     5521 gccgctggtt attgtcattg ccgccgaagc taaaatcccg tttctgaaac tggcgatccc
     5581 ggcagtagca gctgccacta ccgcacattc actgttccca ccgcagccgg gtccggtggc
     5641 gctggtgaat gcttatggcg cggatatggg gatggtttat atctatggcg tactggtgac
     5701 gatcccaagt gtcatctgcg caggtctgat cctgccgaag ttcctcggca atcttgagcg
     5761 cccaacgcca tcattcctga aagcagatca accggtagat atgaataatc tgccctcttt
     5821 cggcgtttcg attctggtgc cgctgatccc ggcgatcatt atgatctcta ccaccatcgc
     5881 caatatctgg ctggtaaaag atacccctgc ctgggaagtg gttaacttta tcggttcctc
     5941 gccgattgca atgtttattg cgatggtggt tgcattcgta ctcttcggca ccgcgcgcgg
     6001 gcacgatatg cagtgggtga tgaacgcttt tgaaagcgcg gtgaagagca ttgcaatggt
     6061 gattctgatc atcggtgcgg gtggcgtgct gaagcagacc atcatcgaca ccggcattgg
     6121 tgacaccatc ggcatgttaa tgtcccacgg caatatctcg ccctacatca tggcatggct
     6181 gatcaccgta ctgattcgtc tggcgacggg tcagggtgtg gtttcagcga tgaccgccgc
     6241 cgggattatc agtg
//
LOCUS       DAEEKQ010000205         6221 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.207, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000205 DAEEKQ010000000
VERSION     DAEEKQ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.207"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            156..932
                     /gene="exuR"
                     /locus_tag="KLK57_003769"
     CDS             156..932
                     /gene="exuR"
                     /locus_tag="KLK57_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HBE0894026.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1277..1939
                     /locus_tag="KLK57_003770"
     CDS             1277..1939
                     /locus_tag="KLK57_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HBE0894027.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPIFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1943..2326
                     /gene="mzrA"
                     /locus_tag="KLK57_003771"
     CDS             1943..2326
                     /gene="mzrA"
                     /locus_tag="KLK57_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HBE0894028.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2473..2841
                     /locus_tag="KLK57_003772"
     CDS             2473..2841
                     /locus_tag="KLK57_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HBE0894029.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2879..3184
                     /locus_tag="KLK57_003773"
     CDS             2879..3184
                     /locus_tag="KLK57_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HBE0894030.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3187..3591
                     /locus_tag="KLK57_003774"
     CDS             3187..3591
                     /locus_tag="KLK57_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HBE0894031.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3581..3880
                     /locus_tag="KLK57_003775"
     CDS             3581..3880
                     /locus_tag="KLK57_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HBE0894032.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4066..4458
                     /locus_tag="KLK57_003776"
     CDS             4066..4458
                     /locus_tag="KLK57_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HBE0894033.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4528..5541
                     /gene="yqjG"
                     /locus_tag="KLK57_003777"
     CDS             4528..5541
                     /gene="yqjG"
                     /locus_tag="KLK57_003777"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005140183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HBE0894034.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYISLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESTEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPQDEMFASVKKRHLT
                     KCL"
     gene            5806..6171
                     /locus_tag="KLK57_003778"
     CDS             5806..6171
                     /locus_tag="KLK57_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HBE0894035.1"
                     /translation="MDWYLKVIKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1570 a         1527 c         1549 g         1575 t
ORIGIN      
        1 tcacaatgac gatacttatt ttttaggaat ttataaatat tcacaatatt aattcaatac
       61 attaattcaa tcaccaaatt tatatatgac caatggcata cccatgaata aagtggtatg
      121 ataacttcgt ccgcattcca ctccgggaga cgcatatgga aatcactgaa ccacgccgtt
      181 tgtatcaaca acttgccgct gacctgaaag agcgcattga acagggcgtc tatctggtgg
      241 gtgataaact gcctgccgaa cgctttattg ccgatgaaaa gaacgtcagc cgaacggtag
      301 ttcgtgaagc catcatcatg ctggaagttg aaggctatgt ggaggtgcgt aaaggttcag
      361 gtattcatgt ggtttccaac cagccgcgcc atcagcaggc ggctgacaat aatatggaat
      421 tcgccaatta cggtccgttt gagttgcttc aggctcgcca gctcatcgaa agtaatattg
      481 ccgagttcgc ggcaactcag gtaacgaaac aagacatcat gaaactgatg gccattcagg
      541 aacaggcgcg cggcgaacaa tgcttccgtg attccgagtg ggatttacag ttccacattc
      601 aggtagccct ggcgacgcag aactccgccc tggcggccat cgttgaaaaa atgtggaccc
      661 agcgtagcca taacccgtac tggaaaaaac tacacgaaca cattgattcc cgtaccgttg
      721 ataactggtg tgatgaccac gatcaaattc tcaaggcgtt aattcgcaaa gatccccatg
      781 ctgctaagct ggcaatgtgg cagcatctgg aaaacaccaa aatcatgtta tttaacgaaa
      841 ccagcgacga tttcgagttc aatgccgacc gctatctatt cgccgaaaac ccggtagtgc
      901 atctcgatac ggccaccagt ggcagtaaat gaaactcctg tccgacaggc gctgcgacgc
      961 gcctgttcgc ttcgtcggta agcgaaaagt ataaagtgtc agcctgtgta aatcctctcg
     1021 ccaccctccc ctgcattcag caaaatcaga ctccacggac atgtaatttt gataacgaac
     1081 aacgttgacc tttgttacaa ttagattcaa tttgaattta tgtttttgaa tgctttctta
     1141 tctcacgatt taacagggaa tagttcaggc tgtgttgatg tatcaaaccc gcagaacata
     1201 ccaaaacagc aataacattg cggtagtgca tcttttaaaa ccagcgtggc gttaaccgat
     1261 tcaccaggaa taatgaatgg aacttttgac ccaattgctg caagccctgt gggcgcagga
     1321 ttttgaaacc ctggccaatc catcgatgat tggcatgttg tattttgtct tgtttgtaat
     1381 tttgttcctt gaaaacggct tgcttccagc ggccttttta ccgggcgaca gtttactggt
     1441 attggtcggc gtgttgattg cgaaaggcgc gatgggttac ccgcaaacga ttctgctgct
     1501 gaccgttgcc gccagcctcg gctgctgggt cagctatatt caggggcgat ggctgggcaa
     1561 tacccgcacc gtacaaaact ggctatctca tttacccgcg cattatcatc aacgcgcaca
     1621 ccatcttttt cataaacacg gtttatcggc gctgttaatt ggtcgcttta ttgcgtttgt
     1681 cagaacactg ctgccgacga ttgccgggtt atcagggctg aataacgcgc gctttcagtt
     1741 tttcaactgg atgagcggtc tgctgtgggt attgatcctg acaactctgg gttacatgct
     1801 cggcaaaacg ccgatatttt taaagtacga ggaccagctg atgtcatgcc tgatgctgct
     1861 cccggtggtg ctgctggtgt ttggcctggc aggttctctg gtcgtgttat ggaaaaagaa
     1921 atatggaaat cgggggtaag ggatgcaaat acctcgcatg tcgcttcgcc agctagcctg
     1981 gtccggcact gttttacttc tggtcggcac gctgttgctg gcctggtccg cggttcgcca
     2041 gcaagagtct acgctggcga ttcgtgccgt tcatcaaggc acaacgatgc cagacggttt
     2101 ttcgatctgg catcaccttg acgctcatgg cattcctttc aaaagtatca cccccaaaaa
     2161 cgacactctg ttaattacat ttgactccag cgaccagagc gccgccgcaa aagcggtcct
     2221 cgacagaaca ttgccccatg gctacatcat tgcgcagcag gacaataaca gtcaggctat
     2281 gcagtggctg acccggttac gggataactc tcatcgcttc ggataacttc ctgttatccg
     2341 aaacatatca ctcactttgg tgatttcacc gtaactgtct atgattaatg aggcggtggt
     2401 taacactacc gcttcgttgt ctggacaatc gttcctttgt aataggtccg aatcacaatg
     2461 gaaggttcaa gaatgaaata ccgcatcgct ttagctgttt ctctctttgc tcttagtgcc
     2521 ggtagttatg ccactaccct gtgtcaggaa aaggagcaaa atatccttaa ggagatcagc
     2581 tatgccgaaa aacaccaaaa ccagaatcgt attgacggtc tgaataaagc cctgagtgaa
     2641 gtccgggcca actgttcaga tagccagctg cgtgccgatc atcacaagaa aatcgcaaag
     2701 cagaaagatg aggtggcgga acgccagcaa gatttagccg aggcgaagca aaaaggcgat
     2761 gccgataaga ttgccaaacg cgaacggaaa ctggcagaag cgcaggaaga gctgaaaaag
     2821 ctggaagcgc gcgactacta actcacaata gtcactactt actcacctgg agaaaactat
     2881 gtcgaaagaa cacactacgg aacatctgcg tgctgagttg aaatcccttt ccgatacgct
     2941 ggaagaggtg cttagctcat ctggcgagaa gtcgaaagaa gagttgagta agattcgtag
     3001 caaagcggag caggcactga aacagagccg ttatcgcctg ggtgaaaccg gtgatgccat
     3061 cgccaaacaa acccgtgtcg cggcggcgcg tgctgatgag tatgtgcgtg aaaatccgtg
     3121 gaccggcgtg ggaattggcg ctgcaatcgg tgtagtgctc ggcgttctgc tgtcgcgtcg
     3181 ttaattatgg cggacactca tcacgcgcaa gggcccggta aaagcgttct gggcatcggg
     3241 cagcgaattg tttccatcat ggttgaaatg gtggaaacac gtctgcggct ggcggtggtg
     3301 gagctggaag aggaaaaagc taatctcttt caacttttac tgatgctggg cctgacgatg
     3361 cttttcgctg catttggtct tatgagcctg atggtgctaa ttatttgggc ggttgatccg
     3421 caatatcgcc tgaatgcgat gattgccacc accgtggtgt tgctgctact ggcactgatt
     3481 ggcggtatct ggacgttacg taaatcgcgt aaatccacct tgctgcgcca tacacgccat
     3541 gagctggcaa acgatcggca actgctcgag gaggagtccc gtgagcagta aagtcgaacg
     3601 tgaacgacgt aaggcgcaac tgcttagcca gatccagcaa caacggctgg atctttccgc
     3661 cagtcgtcgt gaatggctgg aggcaacagg cgcttacgat cgtcgctgga atatgctgct
     3721 aagtctgcgc tcctgggcgc tggttggcag tagcgtgatg gcgatctgga cgattcgcca
     3781 tcctaatatg ctggtccgct gggccagacg cggttttggc gtatggagcg cctggcgtct
     3841 ggttaaaacg accctcaagc agcaacagct tcgcggttaa cttccctctg gccggagcaa
     3901 ttccggcctt atccctcaaa ttttttgaag atttttgaca gttttccttg ctaacaatca
     3961 tcattcacca cgtttatgat tctctccatc gacagcaacg acgctaatac cgcgcccttg
     4021 cacaaaaaaa caatcagcag cctgagtggc gcagtggaga gtatgatgaa aaaattagaa
     4081 gatgttggtg tactggtagc gcgcatttta atgccgattc tgtttattac cgcaggctgg
     4141 ggaaaaatca ccgcttactc tggtacccaa caatatatgg aagcaatggg tgtcccgggt
     4201 tttatgctgc cgctggtgat tctgcttgag ttcggtggtg gtctggcaat cctgttcggt
     4261 ttcctgactc gcaccacagc cctgtttact gcgggcttta cgctgctgac ggcattttta
     4321 tttcacagca actttgctga aggcgtcaac tcgctgatgt tcatgaaaaa cctgacaatt
     4381 tctggcggat tcctgctgct ggcaattacc ggtccgggcg cgtatagcat cgaccgcctg
     4441 ctgaataaaa agtggtaagc acgcgctata cttaacgata aaaaacgagg aggaagctcc
     4501 tcgtttttgc tattggagga gagaaaaatg ggtcaactga ttgacggcgt ctggcatgac
     4561 acctggtacg ataccaaatc taccggcggt aaatttcaac gttcagcttc cgcatttcgt
     4621 aactggctca ctgccgatgg cgcacctggc cccactggca caggcggttt tatcgcagag
     4681 aaagatcgtt atcatctcta tatttcactc gcctgcccgt gggcgcaccg cacgctgatc
     4741 atgcgcaaac tcaaaggact ggaaccgttt atttccgttt ccgtagtgaa cccgctgatg
     4801 ctggaaaacg gctggacctt tgatgacagt tttccgggag caaccggaga tacgctctat
     4861 caacatgaat ttctgtatca gctttatctc cacgccgatc cacactacag cggacgagtt
     4921 actgttcccg tactgtggga caaaaagaac cacaccatcg tcagcaacga atcaacagaa
     4981 atcatccgca tgttcaatac cgcgtttgat gcgctgggcg cgaaagcggg tgattactac
     5041 ccaccagccc tgcaaacgaa aattgacgaa cttaacggct ggatttatga caccgttaac
     5101 aacggcgtgt ataaagctgg ttttgccacc agccagcaag cctacgacga ggcggtggcg
     5161 aaagtgtttg aatcgctggc gcgactggag cagattttag gccagcatcg ttacctgacc
     5221 ggcaaccagc taaccgaagc cgatattcgc ctgtggacta cgctggtgcg ttttgatcca
     5281 gtgtatgtga cccacttcaa gtgtgataag caccgcatca gcgattacct gaatctgtat
     5341 ggcttcctgc gcgatatcta ccagatgccg gggatcgccg aaactgtcaa tttcgatcac
     5401 atccgtaatc attacttccg cagccataag accatcaacc ctacggggat tatttcaatt
     5461 ggtccgtggc aggatctcga tgaaccgcag gacgagatgt tcgcttcggt taaaaaaagg
     5521 caccttacga agtgcctctg ataattagct aagtgcatgt tgttcatatc ggatgcggcg
     5581 tgaacgcctt atcaaaccta caaaacattt atattcaata tgttgcaatg accgcgcagg
     5641 ccagataagc gtagcgcatc tggcgatttt gcgcttgtca tcagtcttag cgcgccttca
     5701 cagcacttta ttaattcctt gaacgaatat ttactgccat ttcattcacg tctattctta
     5761 atttgctgct ttaaaaacaa gtgattgagc aacattgagg caaaaatgga ctggtatctg
     5821 aaagtaataa aaaattatgt cggtttccgg ggtcgtgcgc ggcgcaaaga gtactggatg
     5881 tttattctgg tcaacatcat ctttacgttc gtgctggggc tgctggataa aatgttaggc
     5941 tggcaacgcg ctggtggcga aggcatcctg acgacaatct acggtattct ggtgttttta
     6001 ccgtggtggg cagttcagtt ccgccgcctg cacgacaccg accgctcggc gtggtgggca
     6061 ctgctattct taatcccgtt tatcggctgg cttattatca tcgtctttaa ctgccaggcg
     6121 ggtacgccag gcgaaaaccg ctttggacct gatccaaaac tggaacctta aaacaaatct
     6181 gctcgccgga tgtctgactc atccggcttt tatcatcatg t
//
LOCUS       DAEEKQ010000206         6121 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.208, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000206 DAEEKQ010000000
VERSION     DAEEKQ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.208"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..358
                     /locus_tag="KLK57_003779"
     CDS             <1..358
                     /locus_tag="KLK57_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0894036.1"
                     /translation="PGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWRGGQVLAVPPA
                     YTSQRCACCGHTAKENRLSQSKFRCQVCGYTVNADVNGARNILAAGHAVLACGEMVQS
                     GRPLKQEPTEMIQATA"
     gene            complement(398..1069)
                     /locus_tag="KLK57_003780"
     CDS             complement(398..1069)
                     /locus_tag="KLK57_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HBE0894037.1"
                     /translation="MCGRFAQSQTREDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPFFIYRADGQPIFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="KLK57_003781"
     CDS             complement(1176..1409)
                     /gene="yedF"
                     /locus_tag="KLK57_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HBE0894038.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="KLK57_003782"
     CDS             complement(1406..2611)
                     /gene="yedE"
                     /locus_tag="KLK57_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HBE0894039.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            2798..3211
                     /locus_tag="KLK57_003783"
     CDS             2798..3211
                     /locus_tag="KLK57_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HBE0894040.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEEIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="KLK57_003784"
     CDS             complement(3245..4732)
                     /gene="amyA"
                     /locus_tag="KLK57_003784"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HBE0894041.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="KLK57_003785"
     CDS             complement(4810..5175)
                     /gene="fliT"
                     /locus_tag="KLK57_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HBE0894042.1"
                     /translation="MNHAPHLYFAWQQLVDKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="KLK57_003786"
     CDS             complement(5175..5585)
                     /gene="fliS"
                     /locus_tag="KLK57_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HBE0894043.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="KLK57_003787"
     CDS             complement(5600..>6121)
                     /gene="fliD"
                     /locus_tag="KLK57_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBE0894044.1"
                     /translation="YKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFANGASDSAF
                     KTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTKIATEVKG
                     YLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMMSKLNNTS
                     TYLSQQFTAMSNS"
BASE COUNT         1498 a         1608 c         1527 g         1488 t
ORIGIN      
        1 gccggggcgc aatgtccggg caaaatcagg tttaaaccgt tcgatactgg atcagggctg
       61 gtatgaaatg cgccgccagc ttgagtacaa gcagctctgg cgtggcggtc aggtgcttgc
      121 tgttccgcca gcgtacacaa gccagcgttg cgcgtgctgt ggtcatacag cgaaagaaaa
      181 ccgcctgtca caaagtaaat tcagatgcca ggtatgtgga tatacagtga acgccgatgt
      241 aaatggcgct cgtaacattt tagcggcggg gcacgccgtt cttgcctgtg gagagatggt
      301 gcagtcaggc cgcccgttga agcaggaacc caccgaaatg attcaggcga cagcctgaac
      361 gtagcaggga tcctcgtcct tcagggcgtg gaggatgtca acaaacaggt tgaattaact
      421 ccgcgccctg gtttttgaca ttacctaccg cgcgcgatac cgggtgccag gtgaactggt
      481 ttgctggcac acagccgttg gtagcaattt ctgatgcttc tttaccgcca atctcttgcc
      541 gcatccattc ctgtgcagct tctggcgaca gaaccagcgg gcggcggtca tgaatgtcta
      601 ccagaccctg gtctgctgca gcagtgacaa ttaaaaatcc ttcggcttcg tcaccacgct
      661 caaatggtgt gctacctatc gcggccataa aaataggttg tccatcagca cgatagataa
      721 aaaaaggctg ttttttgtcg ccttcttttt tccactcaaa ccagccatcg gcaaaacaga
      781 ttgcccgacc atgttgccag agcggtttaa acatgcgact ggttgcggca gtttctacgc
      841 gggcgttaat cagcggcggt ttatcccacc atccgggagc atatccccag aaaaccggat
      901 ccagatgaag gtgttcatca cgttcactga gcagaagaac tttggttccc ggcgcgacgt
      961 tgtatctgcc aatgggttcg ggatcgtagg gaatatcgcg ttcaatatct tccgcgagaa
     1021 gcgcaaggta atcttcacgc gtttgggatt gggcaaagcg tccacacata gaaacctcct
     1081 gagatcatac tgaaaagaat aggagagggg gcgggtaaaa tctatgtgtc agaggcaaca
     1141 atgttcccgc tgtgggccac agcgggaatg agggattact tctgaattaa ataacgaatg
     1201 gtcggcccgt cttgctgaat atccagcacc gtatagccgt gattacgcgc atccagtgga
     1261 atattattga tcgactgtgg acagtcgctc accacttcca ggatttcccc tttttttaac
     1321 tgcggcatcg cctcaagggt tgcgactgcc ggataagggc agggttcacc caccatatca
     1381 aggcggtaat caggaacgat atttttcatg cgatctcctt agcagtctgc ggtgccgcac
     1441 ggcggaagaa gcgtttttcc cagccgataa tcaacatcag cgcagtaaac aacagcaaat
     1501 atgtcaccag caggccaccc atcgggccaa aggttttcag caggttgatt ttgtcccagt
     1561 cggtggccag cgccggagcg aaatcatccc agtaatacgc cagaatcgtc gagccgatca
     1621 cattgcccag accgacccac cagtagtgca cctggccttc taccgcgcgg tacatccagc
     1681 cggtttcgca gccgcctgcc agcacgatgc caaaaccaaa cagtaaacca ccaattaccg
     1741 cgtttggtcc cgcccacatg attttgggtt caacgcctaa ctgtacgtaa ctgaagatcc
     1801 cgatggcact caccgccata ccgataatga ttgctttcgc catatgggta cgtccggtga
     1861 tccacatatc gcggaacgcc gaagtaaagc agatttgcgc acgttcaatc agtaaaccaa
     1921 agccgacgcc aaacagcatt gccagcccca gtttgggttg gttcatcgcc gtgagcagcg
     1981 cccagcccag catgccgaaa aagaccagca tcccgagacg aaaacgacgc cgcgcctgat
     2041 ccggtttttg cgtcagcggt gaggcggcag aaactttctg cattttcacg ggaatacgga
     2101 agatgggcag cagggtaaag cgcgcgccaa accatgaacc aatggcagtg gcgatggcaa
     2161 agaaccaggc atgcagcgag aactgaggaa tgccggtaaa gaacgccgcc aggttacagc
     2221 ccattgccag acgcgcgcca aaaccggcga taatgccgcc aataatggcc tgcataatgc
     2281 ggatacggct gcgcggcatg cgcagtttga cattgttggc ccacagcgct gcggcaaagc
     2341 agccgccaaa catgccgagg atcatcatcc cgtcgatgcg ggttaatggc gatccttcca
     2401 gatggataat tttaaaataa ccccactctt cagcatggac gccaaacagc tgcaggagct
     2461 gaccgcccca acgggtaaat tcacccgtga cagcccaaaa ggtgccagta atgccaaaat
     2521 agtaagtgga gagaataccc gccgcgatga ccgcagggat gggtgcccag aatttaatca
     2581 accaggcgtg tttgaattgc tgccatgaca tgaatgtagc ctctgaactc agaaggtttg
     2641 aaacaagaat cgcgaatcat acatcatgca aatttatttt cggggtaata attcacaaaa
     2701 ataatagtta gatcaaattt ctctctgaac ctggaaaggc tggcgacatc agggctttaa
     2761 tgccacaatc ctcttttctt ctcatgcagg acgaatgatg aaaaaactcg caattgcagg
     2821 tgcgctcatg ttgctggcgg gctgtgccga agtcgaaaac tataacaatg tcgtgaaaac
     2881 ccccgcgccg gactggctgg cgggctactg gcagaccaaa gggccgcaac gtgcgctggt
     2941 cagtccggaa gagataggca gcctgattgt cactaaagag ggcgataccc tggattgccg
     3001 tcagtggcag cgggtgattg ccgtgccggg caagttgaca ttgatgtctg atgatctcac
     3061 taacgttacc gtaaaacgtg agctgtacga ggttgagcgt gacggtaaca ccattgagta
     3121 cgatggtatg acgatggagc gtgtggatcg cccgacggca gagtgtgccg ccgcgctgga
     3181 taaagcgccg ttaccgacgc cactgccgta atgaaatctc cccggcttgc cgccggggat
     3241 gaatttaaat cacctcttcg ataacccaca cgctgacgct gccgccgttg caaaagaagg
     3301 tcgcttcgcc gttttcgtcg gtcactacgc tctcttgccg attccccaga aaatcacgcc
     3361 aggttttatt gccgtaattc tctcccagat gaatggtttt ttcgccatca tccccgttcg
     3421 acatgaccac cacgcagccg ggatattcgt cagtgccact gcggctaaag gcaatgcagt
     3481 tcggatggtc gaaaaataac gtctgtacac cgtgggcgaa acgctgacgg gcgagaatta
     3541 actcatcaag ctgttcgatt attggcatat ctatcggata ggtttgcccg tcaccaccga
     3601 catcttcgta atgcgcaccg tagaggtccg gatagaacac cgaaggaacg ccattttccc
     3661 gcaacaaaat tagagcatac gccagcggtt taaaccacgg ttcgaccggc gcttcgaggg
     3721 cttgcaacgg ttgggtgtcg tgattggcta cgagggttac ggcgtggaaa ggatcggctt
     3781 ccactaatgt acccgtgaaa atctgcgtca tgtcgtagtc gcgtcccatg cgcgatgctt
     3841 catggaattt catctgcagc ggcgcatcga acagcatggt tttgccttcc acctgatcaa
     3901 tatacgtttg cagtttatcg acttcatgcg accagtactc tgccacaata aacagcggct
     3961 ttggcgcaac ttcctgtacg tgttcgatcc actctttata aaaccacgcc ggaatatgtt
     4021 taaccgcatc aagacgaaaa ccgtcgcatt gtgtttgttc catcacccag cgcgcccagt
     4081 atttaatctc ttccgtcacg gcatgattgc ggaaatcgat attctcaccc atcagataat
     4141 cgaaattacc gagttcatca tcaacctgat cgttccagcc ttcgccggtg tagtcgttaa
     4201 caattttaaa tatgccatct tcatcaggat tttcgatatg gtcgataccg ctaaaacatt
     4261 tgaagtccca gataaactgc gagtattgcc cggcacgggc ggggaaggtg taacgcgtcc
     4321 agccttcgca ctcaatgatt tcttcatcaa tttgcgtacg gtcatcagca tttacacgct
     4381 gcacgcgaat agcttctttt tcatccgccc ccattttgtg gttgactacc acgtccagta
     4441 gcaccgcaat gtcattacgt ttcagagcgt caatggcggc cagcagttgt gctttatcgc
     4501 catatttagt agggatgctg cctttctgat caaactcacc taaatcaaat aaatcatagg
     4561 agtcgtagcc gaccgaatac ccgcccgatg cgcctttata ggcgggcggc aaccagacca
     4621 tattgatacc aatatcatta aaaccgtcgg cgcgctcggc cagttcaggc cagagcttac
     4681 cgccttccgg gtaataccag tgaaaacatt gtaacagcgt gggattacgc attcttcgac
     4741 tccattcaag gggaacatta gaagcgtagc cgtaatcgga ttattcgcga gccatcggct
     4801 cgttaagatt caaaagaggt tatcctgcgg agccagcaca aagccgccct gatctccata
     4861 ggcacttaac actgattttt gcaccgatga ctgaccgacc agtttcgcca gttcatccat
     4921 acgaatctgt aataactgct ttaccttgct ttcgttgtcg agaatcaggc gcagcatcgg
     4981 gcggagctgc tcctgcatcg tggtggacgg ggcaacctct tcagtcagat gtgcaatctc
     5041 ctgcaccgca ttcacatacg ccatttcgct ggcgatgagt tcgtcccatt gttcttccgt
     5101 tgccaggcgt aacatgagct ggcttttatc gacgagttgt tgccaggcga aatacaaatg
     5161 cggtgcatgg ttcatcagac tgggtcctga atcaaagaag gggagagtaa agactctttc
     5221 caggcatcgg caatattgcg cattaatgct tccacttctt cgactgcgga gacatcgttg
     5281 cgtaaattgg cttgcagcaa gcgcctgacc atatagctat aaagagcaat caagttttgg
     5341 gttagttcgt ctttgctctc ttcatcaaga ctcacccgca gcccgttctc aatgatgttg
     5401 atcgcttttg acaaagagac gcctttgcct tgctgattgt tgtcctgcat aaacaggcgc
     5461 gctctaacca gtgcgctcag cactccatca aatagcatgg tgaccagctg ttgctggctg
     5521 gcgctcatta cggcgctttc gacgccaatt tgtgcatagg cttttgagcc gctcgcggta
     5581 tacatgctga cctccgtgat caggaattac tcattgcagt aaattgttgg ctgagatagg
     5641 tactggtgtt attcagtttg ctcatcatgg tatctaattg cgtaaactgt gcagtgtaac
     5701 gcgccacggt gtcatcaatg ctggcactga cggatagata ctgtttggtc agttttttca
     5761 gtgtggcgtt aatactgtcc tgtgcgctat caataatgcc atcatcggca aggtaacctt
     5821 ttacctcggt ggcaattttg gtggtaattc ctgtttcttt accatcgcct accagcaatt
     5881 cacgtacgga tgctgtattt tcattcagga cttttttgag tttatcatca tcaattttca
     5941 gcttacccgt cgtgccgtcc tgcttaatcc cgatctcgtt taatgttttg aaggcactgt
     6001 cgctggcacc gtttgcaaat tgtgcgcgga tgccgctttg aattgtacgt acaacgctgt
     6061 ctccgagaag tgcaccatta tttttatctt gtgcttcagc acctgggtct acttctttat
     6121 a
//
LOCUS       DAEEKQ010000207         6094 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.209, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000207 DAEEKQ010000000
VERSION     DAEEKQ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.209"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(1..1133)
                     /locus_tag="KLK57_003788"
                     /pseudo
     CDS             complement(1..1133)
                     /locus_tag="KLK57_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466237.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            complement(1133..1315)
                     /locus_tag="KLK57_003789"
     CDS             complement(1133..1315)
                     /locus_tag="KLK57_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894045.1"
                     /translation="MIMLILAPLVGVLGALLLAYGAWLIYPPAGFVVAGALCLFWSWL
                     VARYLDRTRQSVGGGK"
     gene            complement(1327..3060)
                     /locus_tag="KLK57_003790"
     CDS             complement(1327..3060)
                     /locus_tag="KLK57_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HBE0894046.1"
                     /translation="MSRKSYPNVNAANQYARDVVRGKIVACQFVIQACQRHLDDLMAE
                     KSKSFRYRFDKDLAERAAKFIQLLPHTKGEWAFKRMPITLEPWQLFVICCAFGWVNKG
                     TRLRRFREVYTEIPRKNGKSAISAGVALYCFACDNEFGAEVYSGATTEKQAWEVFRPA
                     RLMCKRTPMLTEAFGIEVNASNMNRPEDGARFEPLIGNPGDGSSPHCAVVDEYHEHAT
                     DALYTTMLTGMGARRQPLMWAITTAGYNIEGPCYDKRREVIEMLNGSVPNDELFGIIY
                     TVDEGDDWTGPQVLEKANPNIGVSVYREFLLSQQQRAKNNARLANVFKTKHLNIWVSA
                     RSAYFNLVSWQSCEDKSLTLEQFEGQPCILAFDLARKLDMNSMARLYTREIDGKTHYY
                     SVAPRFWVPYDTVYSVEKNEDRRTAERFQKWVEMGVLTVTDGAEVDYRYILEEAKAAN
                     KISPVSESPIDPFGATGLSHDLADEDLNPVTIVQNFTNMSDPMKELEAAIESGRFHHD
                     GNPIMTWCIGNVVGKNMPGNDDVVKPVKEQAENKIDGAVALIMAVGRAMLYEKEDTLS
                     DHIESYGIRSL"
     gene            complement(3057..3551)
                     /locus_tag="KLK57_003791"
     CDS             complement(3057..3551)
                     /locus_tag="KLK57_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016249564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HBE0894047.1"
                     /translation="MAGTAGRSGRRPKPTARKALAGNPGKRALNKDEPVFTPIKGVEP
                     PEWFAEEDLPLATIMWQLTTKELCGQGLLCVTDLAVLERWCVAYEFWRRAVKNIAIQG
                     NTITGAMGGRVKNPELTAKKEQESEMSSTGAMLGLDPSSRQRLIGLAGQKKATNPFLK
                     IIES"
     gene            complement(3673..4023)
                     /locus_tag="KLK57_003792"
     CDS             complement(3673..4023)
                     /locus_tag="KLK57_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE0894048.1"
                     /translation="MPSLIPRACRKRGCAGTTTDSSGYCDKHRGEGWVQHQRGLSRHQ
                     RGYGSKWTVIRARILKRDKGLCQLCLRVGVVSEAKTVDHIIPKAHGGTDADSNLQSLC
                     WPCHKAKTARERIR"
     gene            complement(4066..4530)
                     /locus_tag="KLK57_003793"
     CDS             complement(4066..4530)
                     /locus_tag="KLK57_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBE0894049.1"
                     /translation="MSRVTAIISALVICIIVCLSWAVNHYRDNAITYKEQRDKNAREL
                     KLANATITDMQQRQRDVNALDAKYTKELADAKAENETLRADVAAGRRRLHIKAVCQSV
                     REATTASGVDNATSPRLADTAERDYFTLRERLMTMQMQLEGAQEYIRTQCIK"
     gene            complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="KLK57_003794"
     CDS             complement(4527..5024)
                     /gene="rrrD"
                     /locus_tag="KLK57_003794"
                     /EC_number="3.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme RrrD"
                     /protein_id="HBE0894050.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPGGNDGLEGVSYIPYKD
                     IIGVWTVCHGHTGKDIMLGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRGAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGKQWKGLMTRREIEREVC
                     LWGQQ"
     gene            complement(5024..5239)
                     /locus_tag="KLK57_003795"
     CDS             complement(5024..5239)
                     /locus_tag="KLK57_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBE0894051.1"
                     /translation="MYQMEKISTGIAYGTSAGSAGYWFLQWLDQVSPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(5920..5994)
                     /locus_tag="KLK57_003796"
     tRNA            complement(5920..5994)
                     /locus_tag="KLK57_003796"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5959..5961),aa:Gly,seq:tcc)
     gene            complement(5998..6073)
                     /locus_tag="KLK57_003797"
     tRNA            complement(5998..6073)
                     /locus_tag="KLK57_003797"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6038..6040),aa:Thr,seq:tgt)
BASE COUNT         1399 a         1748 c         1483 g         1464 t
ORIGIN      
        1 gggacgcgga ttcatatctt ccaggtcgcg gcagtcattg gaagagtaaa ttccccagtt
       61 gatcccggtg gcgtaggctt caaaacggga cttcatatcc ccgcgcagta acgccccggc
      121 gttaaatttg gcgtaataaa cgccctgctt actttttcgt accagtccgg tgttgatccg
      181 ctgttcgatg cgggtcagat acggcaccag tgaatagttg ataaatccca gccccagctc
      241 ttcgatattg ttgaaggtgg cgcgatcggt gttctgcacc atgtgcaacg gcacccggaa
      301 cagacgacag atttcttcca gctgaaactt gcgggtttcc aggaactgac tgtcctcggc
      361 gttcagcgcc atcgacttcc agtccagccc catttccaga atcatcgggc ggtgagcatt
      421 gccaagcccc gtgtgacact cctcaaaatc tttcttcaga cgctcgtaag catccggcgt
      481 gagcttttgt tccgtacgca acacaccgga tgtcaccgca ccattactaa acaacctggc
      541 accgtgctcc tcggttgccg ctgccagtga aatggcctca cgcgcatacg caatgggatt
      601 cagcccgaca agtccatcca gcgtcagggt gcgcacatgc cagatttcat tctgggtcaa
      661 cacatccacg gaaccatccg gaaacgtcac ctgataaacc ggctgccact ggctgttcag
      721 cttcggttcc acacagcccg gatctatcgg aagaagctcc accacttctc ccagtgcctt
      781 taccttgtag gcgtaaaaat tcccccgtag acacaggcag acaataacca gctcccagaa
      841 ttcctgcggt gtcatgtagc cattaggttt tgccgaaacc aacttatgca gtcgttcatc
      901 caccgcccgt gttttcaggg taccgctgat tttgtagaga ctgcagggca gcataccaac
      961 agactctgcc agcaccctga cgcaagaata gaccgccgtc aggcgcatgg cccgctggct
     1021 actgatccgc tttccggtat aggtgtcgta tgacagcccg atagcatcag cagttctgct
     1081 ggcgtggtca ccggcacatc gctttttcgt tgaaataatc ccgaaaagaa cactatttac
     1141 ctccgccgac agactgccgt gtacggtcga gatatcgcgc caccagccac gaccagaaca
     1201 ggcacagcgc cccggcaaca acaaaacccg ccgggggata aatcagccag gcaccatacg
     1261 ccagcaaaag cgcacccagc acgcccacca gaggcgcgag aatcagcatg atcataatta
     1321 cctcagttaa agcgagcgga tcccgtagga ctcaatgtgg tcagacagcg tgtcttcttt
     1381 ctcgtacagc atggctctgc caaccgccat aatcagcgca actgcaccat cgattttgtt
     1441 ttccgcctgc tctttgacgg gtttcaccac atcatcgtta cccggcatgt ttttgccgac
     1501 cacgttgccg atacaccagg tcatgatggg attgccgtca tgatgaaaac gtcccgattc
     1561 aatcgctgct tccagctctt tcatcggatc ggacatattg gtgaagttct ggacgatagt
     1621 gacgggattc aggtcttcat cagcaaggtc atgtgacaac ccggtcgccc cgaaggggtc
     1681 gatgggtgac tcactgaccg ggctgatttt gttcgccgct ttggcctcct cgaggatgta
     1741 gcgataatcc acctccgcac catcggtaac ggtcagaacg cccatttcca cccatttctg
     1801 aaagcgttcg gctgtccgtc gatcttcatt tttctcgacg ctgtacaccg tgtcatacgg
     1861 tacccagaaa cgcggggcca cactgtagta atgcgtttta ccgtcaatct cgcgggtata
     1921 aagtcgcgcc atgctgttca tatccagctt acgcgccagg tcaaaggcca gaatgcacgg
     1981 ctgcccctcg aactgctcaa gggtcagtga tttatcctcg cagctctgcc agctcaccaa
     2041 gttgaaatac gccgaacgcg ccgacaccca gatattgagg tgttttgttt taaagacgtt
     2101 tgccagacgg gcgttatttt tcgcacgctg ctgctgactt aacaaaaatt cgcgataaac
     2161 cgacacgcca atatttggat tggctttttc cagcacctgc gggccggtcc agtcgtcacc
     2221 ttcatcaacg gtatagatga tcccgaacag ttcatcgtta ggcaccgagc cgttgagcat
     2281 ctcgatgact tcccgccgct tgtcgtagca cggcccctca atgttgtacc cggcggtggt
     2341 gatggcccac atcagtggct gacgtcgcgc ccccatcccg gtaagcattg tggtataaag
     2401 cgcatcggtg gcatgctcgt gatattcatc aaccacggca cagtggggtg atgaaccatc
     2461 acctgggttg ccgatcagcg gttcaaaccg cgcgccatcc tccggacggt tcatgtttga
     2521 ggcgttaacc tcaatcccga acgcttccgt cagcatgggt gtgcgtttac acatcagtcg
     2581 cgccgggcga aagacttccc acgcctgttt ctctgtcgtg gcaccggaat acacttccgc
     2641 gccaaactcg ttatcacagg caaaacaata cagggcaaca ccggcagaga ttgctgattt
     2701 gccgttctta cgggggattt cggtgtacac ctcccggaag cggcgcaacc gggtgccttt
     2761 attgacccag ccaaacgcac agcagatcac aaatagctgc cacggctcca gcgtgatggg
     2821 catccgtttg aatgcccact cccccttggt gtgcggcaac agctgaataa atttcgcggc
     2881 ccgttcagcc aggtccttgt cgaagcggta acgaaacgac ttactttttt ccgccatcag
     2941 gtcatcaaga tggcgctggc aggcctgaat cacaaactgg caggcaacaa tctttccgcg
     3001 cacgacatcc cgggcatact gatttgcagc atttacgttg gggtaagatt tccggctcat
     3061 gattcgataa ttttcagaaa cgggttagtg gctttcttct gccccgccag gccaatcaga
     3121 cgctggcggc tgctggggtc gagtccgagc attgcccccg tgctgctcat ctcggactcc
     3181 tgttcttttt tggcggtcag ctccggattt ttgaccctgc cgcccattgc accggtgatg
     3241 gtgttgccct gtatggcaat atttttcacg gcacgtcgcc agaactcata ggccacgcac
     3301 caccgctcaa gtaccgccag gtcagtcacg cacagcaggc cctgaccgca gagttctttg
     3361 gttgtcagtt gccacatgat cgtggcgaga gggagatctt cttcagcgaa ccactctggt
     3421 ggctcaacac ctttgatggg cgtaaaaaca ggttcatctt tgttcagggc tcgcttgccg
     3481 gggtttccgg ccagcgcctt gcgcgccgtt ggcttggggc gacgcccgga acgccccgcc
     3541 gttccagcca tatgcggcac tcctggttaa atttcatttt tcgcgggtat aaaaaaacga
     3601 tggggcgggc agtccggaag acgtcaggtc acagggattt gccccgcccc tccctacaag
     3661 tgagaataat tatcaccgga ttcgttcgcg cgctgttttc gctttatggc agggccagca
     3721 cagactctgc aggttgctgt ctgcgtctgt tccgccatgc gctttcggga tgatgtggtc
     3781 gacggttttc gcctcgctca ccacaccgac acgcagacac aactgacaca gacctttatc
     3841 gcgcttcaga atacgggcac gaatcaccgt ccattttgag ccatagccac gctggtggcg
     3901 gctcagtccg cgctggtgct gcacccagcc ttcaccgcga tgtttatcgc agtaacccga
     3961 actgtctgtg gttgtacctg cacatccacg cttacggcag gcacgtggga ttagtgatgg
     4021 cataaatacc tcataccctg cgaaatgttt accacgataa aaaggctact taatgcactg
     4081 agtgcggata tactcctgtg ccccttccag ttgcatctgc atcgtcatca gccgctctct
     4141 gagggtgaaa taatcccgtt cagcggtgtc tgccagtcgg gggctggttg cattatccac
     4201 gccggaggcg gtggtggctt cacgcactga ctgacagact gctttgatgt gcaaccgacg
     4261 acgaccagcg gcaacatcag cgcgcagagt ttcattttca gctttcgcat cagctaactc
     4321 cttcgtgtat ttagcatcga gtgcattaac atcacgctgt ctctgctgca tgtcagtaat
     4381 ggttgcgttc gccagcttca gttctcttgc gtttttgtcg cgctgctctt tgtaggtaat
     4441 ggcgttatca cggtaatgat taacagccca tgacaggcag acgatgatgc agataaccag
     4501 agcggagata atcgcggtga ctctgctcat tgctgccccc acaaacagac ttcacgctca
     4561 atctcacgac gggtcatcag ccctttccat tgcttaccgc cagcatatgt ccagcgacgt
     4621 agctggtcac atgcgccttt gatatcgccc tggtttattt tgcgaagaag cgtcgatgtt
     4681 ctgaaattgc cagcgcccac gttgtaaacg aacgagtaaa gagcgccgcg cgttgtttcc
     4741 ggtatatcga ctttgatgta cgggttaatt tgtctggcga cagtggcaag gtctttattc
     4801 aggagggctt tgcattctgc ttcggtatac gttttaccga gcatgatgtc ttttccggtg
     4861 tgtccgtgac atacagtcca tacaccaatg atatctttgt atggtatgta gctgacacct
     4921 tccagaccat cgttaccacc tgggccagtg attaacacag atgctatagc aacagcccca
     4981 ccaccaatag cagctgcaac agccttgcgt aatgatggcg acattattca cctctcgcag
     5041 ccttacgctt gtcttctctt attttgaaat acagatttgt cagataagtc agaagcccca
     5101 gaagcagact tcccagcaca ccaatcgcag cccactgtga tggactgacc tgatccaacc
     5161 actgcaaaaa ccagtagccg gcactgccgg cggaggtgcc gtaggcaatg cctgttgata
     5221 ttttttccat ctgatacata tcccgccccc cgacagacct gtgctatcgg aaagaaaaaa
     5281 ggccatcagc aaaactctga tggcctgaat cacctttacc aatattgtat gaaaaaacac
     5341 gcacgactaa ttgacaataa ttttcatttc cattaaaaaa gatcgcgtag cattcttaat
     5401 tcatgaggaa ctttacccgc cagcaatctg agtagcgaaa gctgttcatc cccaatgttt
     5461 ttgctggcgg gtcctttttt ctacggtcct cctccccgga ggggggcatt tcattatttt
     5521 tctaatcgtt actggtgaga aggcggaaga aaaagccagt tcttcgcccc taaataacag
     5581 catgattcca gtccatgcaa aatctgattt ccagctcaca cataccgagc gctgtacaaa
     5641 aaaccgccag ccaaggcagt taagagtgtg ttcccggggt ttgcttagaa tattttgtaa
     5701 gttgtccgaa gagatattta caacaccaga atgatgaatc atcagtcccc tgccagaggt
     5761 tcactacgta acctgaccga caatgcacca ttctggtgct gaaaaaagag cactggaact
     5821 gcaacaaaac aacatcacag aacaggagat ggtaaggagt cagacattcc cacacaatat
     5881 tgtgtcagtg caaataacaa ccttcgtctc aaatctcgct ggagcgggca gcgggaatcg
     5941 aacccgcatc atcagcttgg aaggctgagg taatagccat tatacgatgc ccgcatatgg
     6001 tgccgactac cggaatcgaa ctggtgacct actgattaca agtcagttgc tctgcctgct
     6061 gagctaagtc ggcgctggcc caccaccgag gact
//
LOCUS       DAEEKQ010000208         6035 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.210, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000208 DAEEKQ010000000
VERSION     DAEEKQ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6035
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.210"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(218..964)
                     /gene="cutC"
                     /locus_tag="KLK57_003798"
     CDS             complement(218..964)
                     /gene="cutC"
                     /locus_tag="KLK57_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HBE0894052.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(978..1544)
                     /locus_tag="KLK57_003799"
     CDS             complement(978..1544)
                     /locus_tag="KLK57_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HBE0894053.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSLRLGLNITPLTADHIS
                     LRCHQNATAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLLGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1760..3496
                     /gene="argS"
                     /locus_tag="KLK57_003800"
     CDS             1760..3496
                     /gene="argS"
                     /locus_tag="KLK57_003800"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HBE0894054.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRHSAKVQFGDYQANGM
                     MAVAKKLGMTPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVSTPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGDEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKL
                     QSGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAK
                     GLAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRV
                     LYYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLA
                     DLLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNM
                     LAFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETL
                     TVVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLG
                     LDTLGIETVERM"
     gene            3673..4035
                     /locus_tag="KLK57_003801"
                     /pseudo
     CDS             3673..4035
                     /locus_tag="KLK57_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
     gene            complement(4039..5109)
                     /locus_tag="KLK57_003802"
                     /pseudo
     CDS             complement(4039..5109)
                     /locus_tag="KLK57_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FHIPEP family type III secretion protein"
     gene            complement(5102..>6035)
                     /gene="flhB"
                     /locus_tag="KLK57_003803"
     CDS             complement(5102..>6035)
                     /gene="flhB"
                     /locus_tag="KLK57_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HBE0894055.1"
                     /translation="DPNLILGQIILLIREAMLALLPLISGVVLVALISPVMLGGLVFS
                     GKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTGFYLWHHWPQMMRLM
                     AESPTTAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKKLRMSRQDIRDEFKQ
                     SEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVALQYDENKMSAPKVVA
                     KGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQLYAAVAEVLAWVWQ
                     LKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
BASE COUNT         1569 a         1598 c         1483 g         1385 t
ORIGIN      
        1 atatatattt atcagtttta gcaatttaaa tcccataatc aatgtgaata taaaaaatat
       61 tattagcaca ccgctatgtt cactgtcacc cgctcaacag atcacggtta tcgttcgttt
      121 tttatactgc tcagggataa aaaaaggccc ctgttgaaat tgcaggggcc tggtacgagc
      181 aagcatcata ttgggcgaca tgatgcaacg gtaaaaatca tttggcctga tggcgttcaa
      241 tgattccttt catttcagca accgccgccc cgtctacgat ataacgcgaa tactcgtccg
      301 cgtgttcatc tgatgacatg gacaatcctt gattacgata acgcatcggt gaggcttgcc
      361 acgctcccgc ggagctatgg acttccagca ctccggcatc gaggaagtgg tgcaggtttt
      421 ctgcacggac tcctgctccg gccataatga ttggagcatc acgatgggca ataagttcca
      481 taatttttga taaaccttgc agtgcgtctg atttttgccc tgatgtcagt acccgggcaa
      541 tgcccaattc cgcaagatta ttgagtgtat ataaagggtt agcgcacata tcgaaggcgc
      601 gatgaaaagt cactgccagc ggaccggcag cagccattat tttttccatt cgtggcatat
      661 cgacattccc gtcaacatcg agaacgcccg tcaccagtcc aggaaaacct aattcgcgga
      721 ccgtgcgcac atcctcaaga atggcggcaa actcaccgtc gctgtagcaa aaatcaccac
      781 cgcgtgggcg aattatcgga tgcacaggga tcgtcacccg ctggcgcacg gatttcagta
      841 cccccaacga cggcgttaag cccccctctt ttggggctgc gcataattca actctgtctg
      901 cgccgttttg ctgcgccgtt agtgcacatt ccatgctgta acagcaaatt tccagtaatg
      961 ccatttttgc tccttaatta cgctgactgc tcgctggcaa cgatctcttc aatggaccac
     1021 ggatgaaact taatagttgt tttgccatcg gtgaccgcca gcgtcggatt cggtaatcgc
     1081 tcatgctcac cttttggtga actggtttta actgagatcc ccggtaggct gagtccttca
     1141 tcagaaagca atgccagcgc acgggcattc agcgtttctg gatcacccag cagaacgatt
     1201 tcaatatgtt cccaaccttc gtgtgggtaa cgtttttccc cgggccacgg tagctccaca
     1261 atagaaaact gccagtgcgc aacctgtacc ggttcatgca atttaaacag acaaatcggt
     1321 ctgccattga tcatattttc tgacaaaagc tcgccacact gttcaaaccc gcgacgccag
     1381 cgttcagcag tggcgttttg atggcaacgc aaagaaatgt gatcggcagt cagcggagtg
     1441 atattcaacc ccagacggag ggaaagttca tctaatgcgt gggtaaatcg cggtaaatcc
     1501 gatgcaatat cctgcagctc gtcgatagat tgccagttcg ccataatcac tcttcgtctt
     1561 tcagtaaaag cgttaattta ccctgttgcc ctgcgccaac caaccgctga tttcacgccg
     1621 cttctgatgc aatagtgaaa acggcaatac gccacgcgca cgttgctgac gaaaacagcc
     1681 atttgcagta tactcccgcc ctaatttctt taactggtgc gggcaatttt tgctcgcttc
     1741 atcaatgtaa ggtattccgg tgaatattca ggctcttctc tcagaaaaag tccgtcaggc
     1801 catgattgcg gcaggcgcgc ctgcggattg cgaaccgcag gttcgtcatt cagcaaaagt
     1861 tcagttcggc gactatcagg ctaacggcat gatggcagtt gctaaaaaac tgggtatgac
     1921 accgcgacaa ttagcagagc aggtgctgac tcatctggat cttaacggta tcgccagcaa
     1981 agttgagatc gccggtccag gctttatcaa cattttcctt gatccggcat tcctggctga
     2041 acatgttcag caggcgctgg cgtccgatcg tctcggtgtt tctacgccag aaaaacagac
     2101 cattgtggtt gactactctg cgccaaacgt ggcgaaagag atgcatgtcg gtcacctgcg
     2161 ctctaccatt attggtgacg cagcagtgcg tactctggag ttcctcggtc acaaagtgat
     2221 tcgcgcaaac cacgtcggcg actggggcac tcagttcggt atgctgattg catggctgga
     2281 aaagcagcag caggaaaacg ccggtgatga aatggagctg gctgaccttg aaggtttcta
     2341 ccgcgatgcg aaaaagcatt acgacgaaga tgaagagttt gccgagcgtg cacgtaacta
     2401 cgtggtaaaa ctgcaaagcg gtgacgaata tttccgcgag atgtggcgca aactggtcga
     2461 catcaccatg acgcagaacc agatcaccta cgatcgtctc aacgtgacgc tgacccgtga
     2521 tgacgtgatg ggcgaaagcc tctacaaccc aatgctgcca ggaattgtgg cggatctcaa
     2581 agccaaaggt ctggcagtag aaagcgaagg ggcgaccgtc gtattccttg atgagtttaa
     2641 aaacaaggaa ggcgaaccga tgggcgtgat cattcagaag aaagatggcg gctatctcta
     2701 caccaccact gatatcgcct gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt
     2761 gctgtattac atcgactccc gtcagcatca acacctgatg caggcatggg cgatcgtccg
     2821 taaagcaggc tatgtaccgg aatccgtacc gctggaacac cacatgttcg gcatgatgct
     2881 gggtaaagac ggcaaaccgt tcaaaacccg cgcgggtggt acagtgaaac tggccgatct
     2941 gctggatgaa gccctggaac gtgcacgccg tctggtggca gaaaagaacc cggatatgcc
     3001 agctgacgag ctggaaaaac tggctaacgc ggttggtatt ggtgcggtga aatatgcgga
     3061 tctctccaaa aaccgcacca cggactacat cttcgactgg gacaacatgc tggcgtttga
     3121 gggtaatacc gcgccataca tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa
     3181 ggcagaaatt aacgaagagc aactggctgc agctccggta atcatccgtg aagatcgtga
     3241 agcgcaactg gcagctcgcc tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga
     3301 aggcacgccg catgtgatgt gtgcttacct gtacgatctg gctggtctgt tctctggctt
     3361 ctacgagcac tgcccgatcc tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa
     3421 actggcacaa ctgacagcga agacgctaaa gctgggtctg gatacgctgg gtattgagac
     3481 agtagagcgt atgtaatcga tttttcgtga gagtaaagcc tgatcagggt tacctgatca
     3541 ggctttttta ttgccatcta aatgtattct gaaaatggac atgccattgt tttctgactg
     3601 ttggataaga ggccagaagc gtaatatccg gccccaggga aacgataacg gttgaattta
     3661 aggaataccg cagtgtttaa atttcttgta ttaacattag gcattatctc ttgccaggct
     3721 tacgcagaag atacggttat agaaaacgac catgacattt cagccatcaa agattgttgg
     3781 caaaaaaatt cagatgatga tactgacatt aacgtgatca aatcatgcct gcgacaagaa
     3841 tacaatctcg tcgatgcgca attaaataaa gcctatggtg aagcttatcg ttatatagaa
     3901 caagtgccac gcacaggtgt aaaaaaacct gataccgaac aacttaactt gcttaaaaaa
     3961 tcacagcgag cctggctgga ttttagggac aaagaatgtg aattaatcct ttcaaatgag
     4021 gacgttcagg atttaatgta catcgttcca cgtcgcttcg acaacggtat tattctctgc
     4081 catttttacc ggtttgggtt cggcaggcgc tttttgttcg cgtccgcgta tccaccaggc
     4141 cagcccgagc aatccggcag tgaacagcaa aaataccagg ttcggcattc caggcaccag
     4201 gccgagtaaa ccgagcacgg cggcgcttaa caacataacg cttgggttac tgaaaagctg
     4261 attcaccatc tgctcgccaa catcctgatc ggtgctgaca cgcgtaacga tgacccccgc
     4321 ggcggtaaaa atcaccagcg ccggaatttg tgccaccaga ccatcgccaa tggtcaatag
     4381 cgtataactt tccgccgcgt gtcccatgct catgccatgt tgcagcacgc cgaccagcaa
     4441 cccgccgaca acgttaatga ccatgatgag gatcccggcg atggcatcgc cgcgaacaaa
     4501 cttacttgcc ccgtccattg agccgtaaaa atcggcttcc tgagtcactt cggagcggcg
     4561 ttttttcgcc tcatcttcac caatcaatcc agcgttaagg tcggcgtcaa tcgccatctg
     4621 cttacccggc ataccatcga gaacaaagcg cgcacccact tctgcgatac gcccggcacc
     4681 tttggtaatg accataaagt tgatgatcac gagaatgaca aacaccacga taccgatagc
     4741 gaaattgcca ccaacgagga agtgaccgaa cgcttcgacc accttccctg ccgccgccgc
     4801 gccggtatgc ccttccatca aaatgatacg ggttgaagcc acgttaagtg ccagacgcaa
     4861 caacgtagta aacaacagaa tggtcggaaa cgcagcaaac tcaagcgtgc gctgggtaaa
     4921 catcgccacc agcaacacca tgatcgacaa ggcaatattg aaggtaaaca acaggtcgag
     4981 tatgaatgcg ggcagtggca gcaccatcat cgacaagatc aacaggatca aaatcggtcc
     5041 ggcaaggatc tgccattgtg tcgatttcag gtttgcgggc aggcgcagca tcgcggccag
     5101 attactcatg ggtcggtttc tcgttaataa aatccagggc ttccggcacc ggaagatgag
     5161 taggttgtac agggcgctgt ccaccagcca gacgccagcg tttcagttgc cagacccagg
     5221 ccagcacttc cgccaccgcg gcgtacagtt gacccgggat ttgttgacca atctccgcat
     5281 gtcgatacag cgctcgcgcc agcggcggcg cttcaagcgt cgggacgtta ttttcagcgc
     5341 caatttcacg aatgcgcagc gcgaccagcc ctgcaccttt agcgaccact ttcggtgcgc
     5401 tcattttgtt ttcgtcatac tgcaacgcta ccgaatagtg ggtcgggtta ttgacaatga
     5461 catcagcttt cggcacatcg gccatcatcc gacgccgtgc agcagctcgc tgcatctgac
     5521 ggatccgccc tttaacatga gggtcacctt cgctttgttt gaactcatca cgaatatcct
     5581 gccgcgacat acgcagcttt ttcaggtggc tgaagatttg gaaaaagacg tcaaatccca
     5641 ccattggaat gacaccaagc accaccagca gtgcgcatag cccaaccaga tccatcgcat
     5701 tacccatggc ggtagtcgga gactcggcca ttaagcgcat catctgcggc cagtgatgcc
     5761 agagataaaa ccccgtcacg ctgccaacca ggatggtttt caaaattgcc ttaagcaact
     5821 ccgcgccagt ctgtgccgag aacatccgtt taatgcccgg tagcgggttg agtttggaaa
     5881 acttcggctg caaggatttg ccgctaaata ccagcccccc cagcatgacc ggagaaatga
     5941 gcgccaccag caccacgccg ctaatcagcg gcagcagcgc cagcatggct tctctgatca
     6001 gcagaataat ctgcccgagg atcagattcg ggtca
//
LOCUS       DAEEKQ010000209         5936 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.211, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000209 DAEEKQ010000000
VERSION     DAEEKQ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.211"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(101..796)
                     /locus_tag="KLK57_003804"
     CDS             complement(101..796)
                     /locus_tag="KLK57_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HBE0894056.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAVERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(789..2216)
                     /locus_tag="KLK57_003805"
     CDS             complement(789..2216)
                     /locus_tag="KLK57_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HBE0894057.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(2227..2946)
                     /locus_tag="KLK57_003806"
     CDS             complement(2227..2946)
                     /locus_tag="KLK57_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HBE0894058.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="KLK57_003807"
     CDS             complement(3474..4328)
                     /gene="rclR"
                     /locus_tag="KLK57_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HBE0894059.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLQHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRSAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSREMLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            4554..5879
                     /locus_tag="KLK57_003808"
     CDS             4554..5879
                     /locus_tag="KLK57_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HBE0894060.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIVTILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAMGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         1568 a         1506 c         1305 g         1557 t
ORIGIN      
        1 agcataacca tgacggaacg gggttaagtc aggttgtcct taacgctatt ttcaacctcg
       61 tatgccttct tcaggtttat gttcagatct catatctctc tcagcaatcc tcaataatca
      121 gatacaccgc tttcaccgga ccatgaaccc cgacgacttt gataagctca atatccgccg
      181 ttgagctggg accactgatg atgttaatac atgacggcat tcgttcaccg gcctgcgctt
      241 tctgatgcaa tttttctgcg agttgcgcta cacgcggcag gattgtgctt ttacgcagga
      301 taaaaagaga agattccggg agcaggctca atgaacgccc gcgctcgacg gcggaaaaaa
      361 gaaccacgcc tcccgattcg gttaaaccat attcagcata cacgacaccc actttagcct
      421 gctctgcctg cgagatattc tcggcacctt tcgccggatc ccaaacaacg gcattgcatt
      481 cctgctgcaa acgttcacta atccccaatt cctccagcct cgtgtcaccg ctaatcacga
      541 ccgactgatc tcccagctct ttacacagac gtattgcagc ttctgccgcc ttcgcctcgc
      601 tggtcagctc acagcgcgtc aacataacat cgctggcaaa ctgaataaac gcgtcacagc
      661 gctgctgttg gttaagttgg gtaagccgct cgttagcata gttgttaagc ggcgcatctt
      721 ctgcttgcgg ttcaagtcgc agcgggcgac ccagtgcctg agcaacgtta ttcaaaaatt
      781 cgctccgatt atccattctt tttctcctgc gcctgatgtt tcttaaacca actacggaaa
      841 ctctctccgt cagcttcagg aagatcgcgt gcttccatcc agtcgctaat cgcgccaaat
      901 ttgagtggtg ttttgccgcc attgataaac cagcttgccg catgagcgcc ggccatcatc
      961 ccgactttcc acaatcctgg atgactattg gcataagcga acatttttat cgcccgttgc
     1021 tctgcttttg cggtgatccc tttttcagcc atcacccgac gatgacgcaa aatcagtttt
     1081 gacagcggaa tacgcaccgg acacacgctg tcacaagcag tgcataaaga gcaggcgtag
     1141 ggtaaatctt taaaatcttt atagccgcca agtagcggag aaatcaccgc accaattggc
     1201 cctggataaa tagagccata tccatgaccg ccaatatggc gatatgccgg acaagtattc
     1261 atacaagccc cgcagcgaat acagcgcagc acatcccgaa attcagaggc cagcacctca
     1321 gaacgcccgt tatcgacaat aaccagatga aactcttcag gaccatcaac gtgccccgct
     1381 tcgcgcggtc ctgtcagcca ggtgttgtat cccgtcaaac gtgcaccaac ggcactgcgc
     1441 gccagcatgg tgatcaatac atctacctcg gcaaacgtgg gggcaatacg ctccattccc
     1501 atcacagcaa tatgcgtttt aggcagcgtg gtacacattc gcgcattacc ttcattggtc
     1561 accaggcata ccgaacctgt ctctgccacc gcgaaattac agccggtaat acctatttca
     1621 gcactgagga aatcttcgcg gattttttgc cggatgaata aggtcatcgc ttcaggcgtt
     1681 tccggcccct catagcccag acgttcgtgt agcactcgac ggatctgatg gcgatcttta
     1741 tgaattgccg ggaccacaac atgagatggc ggatcttgat ccagctggag aatatattca
     1801 cccagatcgg tttcaatcac ctgaatgcca gcatcctgca acacatgatt gacaccaatc
     1861 tcttcggtca ccatcgattt agatttcacc accttccggg catttttgcg ttgggcaacc
     1921 tgtaaaatgt agcgggtagc gtcttctttg gtttttgcaa accagacgtg accgccgttt
     1981 tgcgtcactt tttctgagag ctggtacaga taagcgtcga gattactcag aacatgatca
     2041 cgtatctggg cggcccgatc gcgccactcc tcccagtgcc ccaattcatc gaccattttt
     2101 tgccgatttg ccccaatacg ctgctgcgcg tttgccaccg ctttgcgcat gatcggatct
     2161 tcaatttgct gacggatgcg tgtcttaaaa tctgtattac tggttttgat cgacatcttt
     2221 atatcctcag cggctcatca acacttcagc aatatgcatc actttgactt tctgcccttc
     2281 ccgttgtaat cgcccactga tgttcagcag gcaactcacg tcagcaccaa ttaaatactc
     2341 agggcggact tccatcaggt gcacaacctt ttctttcacc atctcgccgg atatttcggc
     2401 cattttgacc gagaacgtgc cgccaaatcc gcagcaggta tcctgttcag caaaggtcaa
     2461 cagctccagt ccacgcacat ttttcagcag cgtaagtggc tcgtccttca ctcccagctt
     2521 acgggccagg ctacaagatg ggtgatacac agctctccct tgcaaactgg cacctacatc
     2581 gactacccct aatttattaa caataaaaga ggtgagatcc tgcatacgcg cggcaacctt
     2641 ttcggcacgt gatgcccatt caggttcatc cgccagatac gtcgggtaac tttttacggc
     2701 ataggtgcaa gagccagccg gtgaaataat gggatcgtcg ttatcctcca gtgcggcgat
     2761 cagatttttc atccctggaa tcgcttcttt gatataaccg ctattgatcg caggctgacc
     2821 gcagcatccc tgtttctccg ggaaatttac gcgacagccg agtttttcca gtagcagcac
     2881 ggagtctcgt gccattcttg atttcagggc gtcaccaata caggtgacaa agaaattgac
     2941 attcacaact aatactccat tacttcatgc ccatttatgc ccgatggttc ccctccttcc
     3001 gggggatcaa tcatcaataa atatggcgtt cttttttaat tggctagcaa cataccaccc
     3061 ctcatttgtg tgacaaatga taatctttca tccaaacttc aaatgcaata actaatttta
     3121 tttctttcgg catcgctcac attttatggg gaaatcgacg ttcatttttg gcataagaaa
     3181 tccatttaaa aaggctacat cccttatacc tattcagagc tacttattca ggggttattg
     3241 agatatacac tgatggtgta caaaaaaatc accgacaata atcacgtatc aagtaatagt
     3301 cggggtttta tttaattgaa gcaatttagt ttgcattttt atttaaaaac tgagctagag
     3361 tccatcagaa aaaaatatat aaagcccaaa ccaccgttgg aaatagaaat agttgaaagg
     3421 ctcacaactc ccggaatgcg gattatcttt gccctgattt ctggcggttt tattcagggt
     3481 gcaagctgtc tgaccctttc ccgatattct cccggagtac aaccaaactc gcggacaaac
     3541 gccttgtgaa aagatgattc actggcatag cctactgact cagcgatcac cacaacaggg
     3601 agcatttccc gggaaaacat ctgggccgct atttgtagac gcaactttgt taatacagcc
     3661 agcggcgtgg ttccggaaac atcacggaaa agctgggcaa aacttgcccg agacatgtgg
     3721 gcgatgctgg ccagcgattc gacggtccag gcgtgtcctg gcatttccag catttgctgt
     3781 attaccgcac caagacgtgg atgcagaagc aaactgagaa tgtttttctc tgtattaacc
     3841 tgtgcaatcc actcacgcac cgcgagggta aagaatgtag cgcagatctg gctacacagt
     3901 gcatccactc ccggcatcgc cgatctggat tcctgttgta aaaacgggat cgcctccctc
     3961 agccagttat attccacgct gtggttaacc ggtgctaaaa acagcgtttc cggcaaagac
     4021 gttaaaaaat aacgcgccga atgttgcaac cgaagagtgc cgcagacaat acaggtcgat
     4081 tcattatcga catgactcag gcgatgagct gaattttgtg gtagcaggac cacatttccc
     4141 gggcgtaatg taaaaatctc tcccgtcggc atttccagct tcgccgctcc ttgcgttaac
     4201 gcatgccaac gaataaccga taattccccg gcaccatgtt gaagctgcca gtcacttcct
     4261 aacacgcaat tcttatcgat cgttccttgt ggagcgttaa gcatcaacaa acggctaagg
     4321 gcatccatat cagactcctg agtggttttt gattacaaac accaacaatc ttagtaaata
     4381 actaatctaa taccatcaaa tggttaatca tgatttttgt tttgtgattc tgattaagaa
     4441 aaagaaaata ccagacgata agacaaaata tcaagcgttg tgaagaaatg gtatttgctc
     4501 ttttgcgtct atagtcatga tgtcaaatga acgtgtttcg acaggaaatc atcatgaata
     4561 aatatcaggc agttattatt ggttttggca aggctggaaa aacattagcc gtcacgctgg
     4621 caaaagcagg ttggcgagtg gctctcatcg aacaatcaaa tgcaatgtat ggcgggacct
     4681 gtattaatat cggctgcatc ccaaccaaaa cattggttca tgacgcacag cagcacacag
     4741 attttgtccg tgccatacag cgtaaaaatg aagtggttaa ttttttacgt aataagaatt
     4801 ttcataatct tgcggatatg cccaatatcg acgtgatcga cggccaggcg gagtttatca
     4861 ataatcatag cctgcgtgtt catcggcctg agggaaatct ggaaattcat ggcgagaaaa
     4921 tttttattaa taccggtgca caaaccgtgg ttccgccaat tcctggaatt accaccacgc
     4981 ctggtgtata tgacagcacc ggattactta atctaaaaga attgcctggg catttaggta
     5041 ttttgggcgg cggatatatt ggcgttgagt tcgcctctat gttcgctaat tttggcagca
     5101 aggtaaccat tttagaagca gcttcgctgt ttttgcctcg ggaagatcgg gatattgctg
     5161 ataatatcgt gacgatttta cgcgatcagg gcgtcgatat tatcctcaat gcccatgtgg
     5221 agcgaatcag tcaccatgaa aatcaagtgc aagtgcatag cgagcacgcc caactggcgg
     5281 tggatgcact gttaatagct tccggtcgtc aaccggctac cgcttcgtta catccagaaa
     5341 atgccggtat cgcagtaaac gagcgcgggg caattgtcgt tgacaagcaa ttacatacca
     5401 ccgcagacaa tatttgggcg atgggagatg ttaccggcgg gctgcaattt acttatattt
     5461 cactggatga ttaccgcatt gtgcgtgatg agttgctggg agaaggcaga cgtagtactg
     5521 atgatcggaa aaatgtgcct tattccgtat tcatgacacc gccactgtcc agggttggta
     5581 tgacagaaga acaggccaga gagagtggtg ctgatattca ggtggtgaca ttgcctgtag
     5641 ctgcaattcc gcgtgccaga gtgatgaatg atactcgtgg ggtattaaaa gcgattgttg
     5701 ataataaaac ccaacgtata ttaggggcat cactgctgtg tgttgactcc cacgagatga
     5761 tcaatatagt gaaaatggtg atggatgccg ggctgcctta tagcatatta cgcgatcaga
     5821 tatttactca tccgtcgatg agcgaatcac tcaatgatct attttcatta gtcaaataaa
     5881 ctcaaaatca gacgccagaa caaatattct ggcgtctcag agaaaagaat cttatt
//
LOCUS       DAEEKQ010000210         5931 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.212, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000210 DAEEKQ010000000
VERSION     DAEEKQ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.212"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            292..368
                     /locus_tag="KLK57_003809"
     tRNA            292..368
                     /locus_tag="KLK57_003809"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:326..328,aa:Arg,seq:tct)
     gene            complement(413..1174)
                     /gene="envY"
                     /locus_tag="KLK57_003810"
     CDS             complement(413..1174)
                     /gene="envY"
                     /locus_tag="KLK57_003810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HBE0894061.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKNLTCVKPWSRLATPVIACHSRTPEVFRLAA
                     NHSKQQPSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENISYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYGLTPLNYLAKQRQKVMW"
     gene            complement(1357..2247)
                     /locus_tag="KLK57_003811"
     CDS             complement(1357..2247)
                     /locus_tag="KLK57_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HBE0894062.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="KLK57_003812"
     CDS             complement(2248..5220)
                     /gene="nfrA"
                     /locus_tag="KLK57_003812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HBE0894063.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPSAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGSNALYWWLHAQRYIPGQPELALNDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKRLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="KLK57_003813"
     CDS             complement(5207..>5931)
                     /gene="nfrB"
                     /locus_tag="KLK57_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase family protein"
                     /protein_id="HBE0894064.1"
                     /translation="DTALRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESID
                     AWQIPSSLIAEMPASVALHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRY
                     FIVLRGQIVTGLRHWYARRRGHDPRAMLYNAVQHQWLTEQQAGEIWRQYVPHQFLFAE
                     ILTTLGHINRSAINVLLLRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQS
                     LLLKAGLNTEQVAQLESENEGE"
BASE COUNT         1347 a         1559 c         1550 g         1475 t
ORIGIN      
        1 ttctgatgat aggatgagta tccataatga tcaccgacgt tggtttcata gttaccagtc
       61 tcataggagc ggacaatttt ccgttagggc ggtaaattgt actttgatac atgaaaatac
      121 gggttttctt gattcaaacg cgcagcggtg tgcatttgtt tgccgctata gcgtaataaa
      181 tcagaaaatc agacgcggtc gttcacttgt tcagcaacca gatcaaaagc cattgactca
      241 gcaagggttg accgtataat tcacgcgatt acaccgcatt gcggtatcaa cgcgccctta
      301 gctcagttgg atagagcaac gaccttctaa gtcgtgggcc gcaggttcga atcctgcagg
      361 gcgcgccatt tcttcttcat ttaaccccgt cttatccgtt ccgctttgct cttcaccaca
      421 tcactttttg tcgctgtttg gcgagataat tcaacggtgt caggccgtaa aacgccttaa
      481 aaacagagat aaagtacgac gtgctgctat agccacataa ttgcgccacc tgagtgatat
      541 ttttgttatc catcaataac atctgtacgg cgtaacgcat acgacactct gtgacaatct
      601 ggctatagct gatattttcg ttttttaatt tctttttgag caggctgggg cttaaacata
      661 gcgaactggc gacaattcgc agattccagt aatgctgaat atcgctttga ataatgcggc
      721 agacgctgtc gcggacgctg ctgcgtaaga tatacatcag tagggcaata aaccgcgatt
      781 gctcaagaaa gttagacaat acggtaaaaa gcaatgcgcg cgtcaactcc gcttcgcagg
      841 gtttgctggg ttgctgcttg ctgtggttgg cggctagccg gaacacttcc ggtgtacggc
      901 tatgacaagc gataaccggg gttgccagcc gcgaccaggg ctttacacag gttaagttct
      961 tatttaaaaa caacagataa tcgttgatga tgttacggtt gatgtgggtg attaatacgt
     1021 gatttaacgt tgagagttca atgacgttat tgttgcacgc gaaggcggcc aggtagtttt
     1081 tcggaagggt aaacgtagca tggttattga cgcttacctc tacctctttt tcggtcagga
     1141 tcaccacgca aggttcactg ctgctcaatt gcatttcgca ctcctcagat atcagaaact
     1201 ccgctcaaag gatctatgct tcctgcatga gtgatcggcc cgttcgccga taacgatctt
     1261 ctttctttag cacgcttttt agcaattaat cttgatggaa ttctgacgag agcgaaagag
     1321 gtaagccagg tcgtacccga cttacctgga ggagatttaa tactcgagaa tgccgtgcgc
     1381 gacgggcaaa tagcgcagag agaaatagag agtgtcttta ccgcaagagg aacgtttcgc
     1441 taacagcgag gcgatctctg catccggttt cggtttcgct gtaaaggttt tgcctttgcc
     1501 ggcgacaaac agttcataaa caatgccgct tgctggggcg gagctttggc aatcggcgct
     1561 ggcctgtaga taacgttcac gctgttcagc ggtaagttta tccacgccgc tgccatcctg
     1621 tacccagaca ttaactccgg ttgctttaac ttgttccagc agttggcgat agccatctgg
     1681 cgacatgttt ccggcgaaaa aactactgat ataaaccggt tttgctgaaa catcgctaat
     1741 cagccgctgc gcgttgttta accatgttag caaaggctga cgggcagctt cgctgcgcca
     1801 gttcaggtcg tcaatttccg cgctgatgta ccagccatcc ggcgttatgc caggcgcggc
     1861 gctccataat ctggcttgct ggagatcggc agccagcagg cgattaagat agctttccag
     1921 cgctgcggac gactgtttct ggtgcataaa aaattccgga tcggcgttca gcccgacaat
     1981 cagtttcagg ccagcctgtt gcgcagctgc ggcacgctta aacaataacg tgcgctgttc
     2041 tggctgggta aatgcatcgc cgtaacgggt ccattgcaaa accagggtat cgaagccttg
     2101 caaacgtaac tgactcatca gcccctgcca ctgggtatcg gtaacctgac tatctcggtt
     2161 ttgtggttgc cagataatac ctttcatcgc aaaggaaaaa gggctgacca aaagcagtgt
     2221 cagcaatacg aaaatgaact tacgcattta ccagtgcact ccaatggtga gaaacgcgtt
     2281 gttgcgctct ccgttacgtt gattaatcgc cttaaaggta tgttgatact cgacgccgag
     2341 actgactttg tgcggccagg cgtcgtagtg cgtctcgccg gtccagatat tccagcggac
     2401 cccgactccg ccaagctgcg cgccctgagt gcctttatca cgatagccgt tgtcctgaac
     2461 gtgagcgtaa ggctcaatag tctgtccgtt agctaccttc tgatgccagc tgacgcgata
     2521 atctgccgtc cacgcctgaa tatcctggcg gatatattgc gccgcatcga ggtacaggtt
     2581 ttgggcaaac cagcctgaac cgttcgggtg ccattcgtcg ctgtatttgc cgccattaaa
     2641 gaatgaggcg ctggcgcgca gcatggtatc ggatgcgcca ttttgcccgt tcagcggcaa
     2701 ctgctgttcg acggcgagga aaaagatctg atcgcgcagc ggcttccagc gcagaccggt
     2761 gccggacatc ggatttttca ccggcatcat caccccgttt tctccggtat cggcaaagac
     2821 gcggctataa actgagagca ggtcgccttc cagcagcata ttgcgtccga tgcggtactc
     2881 ggcttccagt tgcccgtagc tacgatagct tttccctggc gctgcgccgc caacattatt
     2941 gttagcggta ctcattgccc cggaacgcaa gccgatagaa gaatcgaaac tgaacgtcca
     3001 gcggcgaccg acctcctcat gcaaacggcg gaaattgaag cgttgttgat tttgctctgg
     3061 ggtcagtggg gttatcagag cctgattatc aatgtcatca atcaccagcc gggcgtagtg
     3121 ctgcgtcgca ggcatgtcat ccagacgctg gttcacgtag gccagttgtc ggatcagtgc
     3181 cggatcgtcc ggaagccttt tatgcgcctg ttcgagcatt tcccgcgact gtgcgatatc
     3241 accgctatcc cacaaggcgt aaccaagcgc tgcctggatg ttgctattat tcggttccag
     3301 ttccagcgcg gcgcgcaaat cactcaccgc ggccgggaca ttatgacgtt ggcgataaat
     3361 tgtcgcccgc gcaacgtaag cgttggcaga aggcgcaata ttgattgagc gcgtgagatc
     3421 gttcagtgcg agttccggct gaccaggaat gtaacgttgt gcatgcagcc accagtagag
     3481 ggcattgctt cccagtccac gttgttctgc ctgttgtagc cagcgatcgc gagccgcacc
     3541 atttcctgcc gcctgggcgg tattggcagc agcaagcaga tcctcattgc tcatgtcgtg
     3601 aagactgatt ttctgccagg ccgccagtgc ggtggcgtag tcctcaacct gatacgcctg
     3661 ataggctacc gcacgatgtt gccaggcgct cggttgtcgt tgttcggcct gaagccatgc
     3721 atacaacgcc acaccgggta gcgtgtcccg ataacacttt gccagacggt tccaggcggc
     3781 agcatcgtag gaaggcgaca tatcgcccag caagcgaact attgccgggc aattatctgc
     3841 aatacccggc aactgacttt gccactgacg ttgctccgcc agcggtaagg gtttcgataa
     3901 aatcgccacc ttcgccggcg ttgccaggta aggatgactt tccagcagag acgccagtcg
     3961 cgccattaaa gtctggctga cacgcgcatc gccctggaaa ggatagcgtt gcagcaataa
     4021 atcggcagct tcgcgtgact gctcgttctg catcagttgc caggtcagtt gatccaggcg
     4081 ggtaagattt gccggttctt gctgatacag caatcgtgcc agacgcagag attcagcctt
     4141 gttacgggtc gccacgctga cagcataacg ctcctcaagc atttcattgg cggggagggt
     4201 ggcgagcagt ttttgcgctg cgtcgtactg accttctttt aacagcaccg gtagcgtcgc
     4261 gccaacaaca tactggcggt tatcggcaaa ctgcaccgta taattcgcca atgcctgaac
     4321 ggggttggcg ctgtatttag ataacagata gagccaactt ttctcttgtg cgtccgtggt
     4381 aaacagcggc ttattttcat tgagataatg ctggaggcgt gctttttcgc cacgataagc
     4441 cagcgcggtc gcgtaagtaa tatatgactg aggatcggtg aagatcccct gtgattgcag
     4501 tgctaggatc cgatcgtcca gctgcccggc aagaagcacg tcaaaccact gacggcgttc
     4561 tgccgcgctt aatgtgttct gctggcgtgc ttcattgtat agcgtatctg cctgggacca
     4621 ttgtttcagg tagattgccc gttgcagcag atcggttcgc agcgtttttc cttccggcga
     4681 tgcagcaaac gtcgcatcgt tcagttgcgc tctggcgaca ggtaactgtg caagccgcag
     4741 ggcattctgc ccgacttcac tgcgacaacg cagggtcggc gcagcatcgc acgctttttg
     4801 ctgggcaagc agttcttcaa cagttgtaac gcttttcact tcaaccggaa tagccgccag
     4861 actgcgctca agtcgggcat ctcctgggtg acgtttcagt tgatcctcaa gcaacagccg
     4921 cgcccggtca tcatgaccaa aatggcgata ggcttccgca aggtataaag tcagcggaat
     4981 attatccggc acctgctggt gtatatattc aaattcgcgg atggcggttg cttcgtcgtt
     5041 atttttctgt gccttcagcg ccttatcgag acggggataa ataacaaaat ggcgataatc
     5101 gctcagcccc agctcttctg cgctggtgcc gatattgtct gcgagtgcgc tggtactcaa
     5161 taaagacgtc agcagtaaac cagaccatcc gatgacgcga ttaaggttat tctccttcat
     5221 tttcggactc cagttgcgca acctgttctg tgtttaaacc tgctttgagt aatagtgatt
     5281 gcatcgaaac ttgtaattcg cgttgaattg tcaggacgcg atccaacgtt tcctggctga
     5341 taacgccttc ggtgaccaaa aacttgccga gcggcagaga actgcgttca tggcgcaata
     5401 acaacacgtt aattgctgaa cgattaatat gaccgagcgt ggtcagtatt tcggcgaaca
     5461 ggaactgatg cggcacatat tgccgccaga tttcaccggc ctgctgttcc gtgagccact
     5521 gatgctgaac cgcattgtac aacattgccc gcggatcgtg accgcgtcgg cgtgcatacc
     5581 agtgacgtaa ccccgtgaca atttgtcccc gcagaacaat gaagtaacgc actttgcgtc
     5641 cgactttacg cgtcagggcc gccagcgaaa ccgggtcaat accatcttca ctgccgacaa
     5701 ttaactcatc attttccaga cgcagcggca gtaccgcata atgcagcgcc acggaggccg
     5761 gcatttcggc aatcagcgag gaagggatct gccaggcatc gatggattcc cacgccacgc
     5821 cgttttgctc tgccagcgcc tgtgccagct gctcggcgct aatcagcccc tgcatcagca
     5881 ttgaaccgcc caagcgtaga ccttcgacgc gattacgcag tgctgtatcg a
//
LOCUS       DAEEKQ010000211         5779 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.213, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000211 DAEEKQ010000000
VERSION     DAEEKQ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5779
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.213"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(120..596)
                     /locus_tag="KLK57_003814"
     CDS             complement(120..596)
                     /locus_tag="KLK57_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HBE0894065.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(655..1944)
                     /gene="bioA"
                     /locus_tag="KLK57_003815"
     CDS             complement(655..1944)
                     /gene="bioA"
                     /locus_tag="KLK57_003815"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HBE0894066.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVVSAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKICDREGILLIADEIATGFGRTGKLFACEHAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARDAEMVADVRVLGAIGVVETTHPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2031..3071
                     /gene="bioB"
                     /locus_tag="KLK57_003816"
     CDS             2031..3071
                     /gene="bioB"
                     /locus_tag="KLK57_003816"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HBE0894067.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            3068..4222
                     /gene="bioF"
                     /locus_tag="KLK57_003817"
     CDS             3068..4222
                     /gene="bioF"
                     /locus_tag="KLK57_003817"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HBE0894068.1"
                     /translation="MSWQEKINAALDARRTADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWQQGAEKFGVGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4209..4964
                     /gene="bioC"
                     /locus_tag="KLK57_003818"
     CDS             4209..4964
                     /gene="bioC"
                     /locus_tag="KLK57_003818"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HBE0894069.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHAELQRRSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            4957..5634
                     /gene="bioD"
                     /locus_tag="KLK57_003819"
     CDS             4957..5634
                     /gene="bioD"
                     /locus_tag="KLK57_003819"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HBE0894070.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
BASE COUNT         1355 a         1546 c         1627 g         1251 t
ORIGIN      
        1 actgtttctc gttcagcttt ttatactaac ttgagcgaaa cgggaaggta aaaagacaaa
       61 aagttgtttt taataccttt aagtgatacc agatggcatt gcgccatctg gcagagtgat
      121 taactaaaca tcgcagtaat cgaggcgctt gccagagagt ggaaatgaac gttaaacccg
      181 accatcgcgc cgctggcacc ttcatcgaca tcaatacgtt ctatatccag cgcgtgaacg
      241 gtaaaaatgt agcgatgagt ttcgcctttc ggcggtgctg cgccatcgta cccggtttta
      301 ccaaagtcgg tacgcgtctg caaaacgccg tctggcattg ctaccagacc agagccaaac
      361 ccttgcggta atacgcgggt atcagcgggt aagttaacaa ctacccagtg ccaccagccg
      421 gagccggttg gcgcatccgg gtcgtagcag gtgacaacaa aacttttcgt tcccgcagga
      481 acatcatccc acgccagatg cggtgaaata ttatcgccat cgtaacccat gccgttaaag
      541 acatgacgat gcggcaattt atcgccatcg cgcagatcgt tactgatgag tttcatgaac
      601 cctcctttct tgtttgcaga aagtgtagcc agaaaccctc acgcggactt ctcgttattg
      661 gcaaaaaaat gtttcatcct gtaccgcgcg gttaaccgct gcggtcagac gctgcaactg
      721 ttgcgggaga ataatatagg gcggcatcag gtaaatcagt ttgccaaaag gccggatcca
      781 gacaccctgt tcgacaaaga atttttgcag cgccgccata ttcaccggat gagtggtttc
      841 gaccacgcca atggccccca gtacgcgcac atcggcaacc atttcggcat cacgggcggg
      901 ggcaagttgc tcgcgcagct gtacttcaat atccgccacc tgttgctgcc agtcgccaga
      961 ttcgagaatc gccaggctgg cgtttgctgc cgcgcaggcc agcggattgc ccataaaagt
     1021 tggcccatgc ataaagcaac cggcttcacc gttactgatg gtttctgcaa cctcgcgcgt
     1081 ggtgagtgtg gcggaaaggg tcattgtgcc gccggttaag gctttaccga ggcacaaaat
     1141 gtccggcgcg atttctgcat gttcacaggc aaacagtttc ccggtacgac caaatccagt
     1201 ggcgatctcg tcggcaatca gcaagatacc ttcgcgatcg catattttgc ggattcgttt
     1261 taaccattcc ggatggtaca tgcgcatccc gcctgcgccc tggacaatcg gctcaatgat
     1321 caccgccgcg atttcatgac gatgcgccgc catcaggcgg gcaaagccca ccatatcgcg
     1381 ctcatcccat tcgccatcca tacggctttg tggggcggga gcaaacaagt tttctggcag
     1441 atagcctttc cacaggctgt gcattgagtt gtccggatcg cacaccgaca tcgcgccaaa
     1501 ggtatcgcca tgataaccat tgcggaaggt cagaaaacgc tggcgcgctt cgcctttggc
     1561 ttgccagtac tgcaacgcca ttttcatcgc cacttccacc gctacggaac cggagtccgc
     1621 gagaaaaacg cactccagcg gttgcggcgt catcgccacc agtttgcggc acagctcaat
     1681 ggctggcgca tgggtgatac cgccaaacat cacatgcgac atggcatcaa tttgcgactt
     1741 catcgccgca ttaagctgcg ggtgattgta gccgtggatc gccgcccacc aggacgacat
     1801 accgtcaacc aggcgtctgc cgtcagacaa aatcagctcg caaccttcgg cgctcaccac
     1861 cggataaacc ggcagcgggg aggtcatgga tgtgtatggg tgccagatat ggcgttggtc
     1921 aaaggcaaga tcgtccgttg tcataatcga cttgtaaacc aaattgaaaa gatttaggtt
     1981 tacaagtcta caccgaatta acaacaaaaa acacgttttg gagaagcccc atggctcacc
     2041 gcccacgctg gaccttgtcg caagtcacag aattatttga aaaaccgttg ctggatctgc
     2101 tgtttgaagc gcagcaggtc catcgccagc atttcgatcc tcgtcaggtc caggtcagca
     2161 cgttgctgtc gattaagacc ggagcttgtc cggaagattg caaatactgc ccgcaaagct
     2221 cacgctacaa aaccgggctg gaagccgagc ggttgatgga agttgaacag gtgctggagt
     2281 cggcgcgcaa agcgaaagcg gcaggatcga cgcgcttctg tatgggcgcg gcgtggaaga
     2341 atccccacga acgcgatatg ccgtacctgg aacaaatggt gcagggggta aaagcgatgg
     2401 ggctggaggc gtgtatgacg ctgggcacgt tgagtgaatc tcaggcgcag cgcctcgcga
     2461 acgccgggct ggattattac aaccacaacc tggacacctc gccggagttt tacggcaata
     2521 tcatcaccac ccgcacttat caggaacgcc tcgatacgct ggaaaaagtg cgcgatgccg
     2581 ggatcaaagt ctgttctggc ggcattgttg gcttaggcga aacggtaaaa gatcgcgccg
     2641 gattattgct gcaactggca aacctgccga cgccgccgga aagcgtacca atcaacatgc
     2701 tggtgaaggt gaaaggcacg ccactggcgg ataacgatga tgtcgatgcc tttgatttta
     2761 ttcgcactat tgcggtagcg cggatcatga tgccgacctc ttacgtgcgc ctttctgccg
     2821 gacgcgagca gatgaatgaa cagactcagg cgatgtgctt tatggctggc gcaaactcga
     2881 ttttctacgg ttgcaaactg ctgaccacgc cgaatccgga agaagataaa gacctgcaac
     2941 tgttccgcaa actggggcta aatccgcagc aaactgccgt gctggcaggg gataacgaac
     3001 aacagcaacg tcttgaacag gcgctgatga ccccggacac cgacgaatat tacaacgcgg
     3061 cagcattatg agctggcagg agaaaatcaa cgcggcgctc gatgcacggc gtactgccga
     3121 tgccctgcgt cgccgttatc cggtggcgca aggagccgga cgctggctgg tggcggatga
     3181 tcgccagtat ctgaactttt ccagtaacga ttatttaggt ttaagccatc atccgcaaat
     3241 tatccgcgcc tggcagcagg gcgcagaaaa gtttggtgta ggtagcggcg gttccgggca
     3301 tgtcagcggt tatagcgtgg tgcatcaggc gctggaagaa gaactggccg agtggcttgg
     3361 ctattcgcgg gcgctgctgt ttatctctgg ttttgccgcg aatcaggcag tcattgccgc
     3421 gatgatggcg aaagaggacc gtattgttgc cgaccggctt agccatgcct ccttgctgga
     3481 ggctgccagt ttaagcccgt cgcagcttcg ccgttttact cataacgatg tcgctcatct
     3541 ggcgcgactg cttgcttccc cctgtccggg gcagcaactg gtggtgacag aaggcgtgtt
     3601 cagcatggac ggcgatagcg cgccactggc ggaaatccag caggtaacgc aacagcacaa
     3661 tggctggttg atggtcgatg atgcccacgg cacgggcgtt atcggggagc aggggcgcgg
     3721 cagctgctgg ctgcaaaagg taaaaccaga attgctggtg gtgacttttg gcaaaggatt
     3781 tggcgtcagt ggggctgcgg tgctttgctc cagtacggtg gcggattatc tgctgcaatt
     3841 cgcccgccac cttatctaca gcaccagtat gccgcccgct caggcgcagg cattacgtgc
     3901 gtcgctggcg gtcattcgcc gtgatgaggg ggatgcacgg cgcgaaaaac tggtgtcact
     3961 cattgcgcgt tttcgtgccg gagtacagga tctgccgttt acgcttgctg attcatgcag
     4021 cgccatccag ccattgatcg tcggtgataa cagccgtgcg ttacaactgg cagaaaaact
     4081 gcgccagcaa ggctgctggg tcacggcgat tcgcccgcca accgtacccg ctggtactgc
     4141 gcgactgcgc ttaacgctaa ccgcagcgca tgaaatgcag gatatcgacc gtctgctgga
     4201 ggtgctgcat ggcaacggtt aataaacaag ccattgcagc ggcatttggt cgggcagccg
     4261 cgcactatga gcaacacgca gagttacagc gccggagtgc tgacgtctta ctggcaatgc
     4321 tcccacagcg taaatacacc cacgtactgg acgcggggtg tgggccaggc tggatgagcc
     4381 gccactggcg agagcgtcac gcacaggtga cggcgttaga tctctcaccg ccaatgcttg
     4441 tgcaagcccg ccagaaagat gctgcagacc attatctggc gggagatatc gaatccctgc
     4501 cgttagcgac tgcgacgttc gatcttgcat ggagcaatct cgcggtgcag tggtgcggta
     4561 atttatccac ggcactccgc gagctgtatc gggtggtgcg ccccggaggc gtggtggcgt
     4621 ttaccacgct ggtgcaggga tcgttacccg aactgcatca ggcgtggcag gcggtggacg
     4681 agcgtccgca tgctaatcgc tttttaccgc cagatgaaat cgaacagtcg ctgaacggcg
     4741 tgcattatca acatcatatt cagcccatca cactatggtt tgatgatgcg ctcagtgcca
     4801 tgcgttcgct gaaaggcatc ggtgctacgc atcttcatga agggcgcgac ccgcgaatat
     4861 taacgcgttc gcaattgcag cgattgcaac tggcctggcc gcaacaacag gggcgatatc
     4921 ctctgacgta tcatcttttt ttgggagtga ttgctcgtga gtaaacgtta ttttgtcacc
     4981 ggaacggata ccgaagtggg gaaaaccgtc gccagttgtg cacttttaca agccgcgaag
     5041 gcagtaggtt atcggacggc aggttataaa ccggtcgcct ctggcagcga aaagaccccg
     5101 gaaggcttac gtaatagtga cgcgctggcg ttacagcaca acagcagcct gcagctggat
     5161 tacgcaacag taaatcctta caccttcgca gaacccactt cgccgcacat catcagcgcg
     5221 caagagggca ggccgataga atcattggta atgagcgccg gattacgcgc gcttgaacaa
     5281 caggctgact gggtgttagt ggaaggtgct ggcggctggt ttacgccgct ttctgacact
     5341 ttcacttttg cagattgggt aacacaggaa caactgccgg tgatactggt agttggtgtg
     5401 aaactcggct gtattaatca cgcgatgttg actgcgcagg caatacaaca cgccggactg
     5461 actctggcgg gttgggtggc gaacgatgtt acgcctccgg gaaaacgtca cgctgaatat
     5521 atgaccacgc tcacccgcat gattcccgcg ccgctgctgg gagagatccc ctggcttgca
     5581 gaaaatccag aaaatgcggc aaccggaaag tacataaacc ttgccttgtt gtagccattc
     5641 tgtatttggt taaattgcga gcgagatcgc gtcttcgatt gactgcaatt taaccaatta
     5701 aattctaaaa taatcacgaa aaaaatttta cttctgcctc atgcggcgaa tgtgggaatt
     5761 gcccaggcgg cgggggata
//
LOCUS       DAEEKQ010000212         5697 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.214, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000212 DAEEKQ010000000
VERSION     DAEEKQ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5697
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.214"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            34..657
                     /gene="nfeR"
                     /locus_tag="KLK57_003820"
                     /gene_synonym="yqjI"
     CDS             34..657
                     /gene="nfeR"
                     /locus_tag="KLK57_003820"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HBE0894071.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHSRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDITDAQIKKIIAVIDHAAFDITQLD"
     gene            complement(764..2284)
                     /gene="aer"
                     /locus_tag="KLK57_003821"
     CDS             complement(764..2284)
                     /gene="aer"
                     /locus_tag="KLK57_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HBE0894072.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            2702..4081
                     /gene="ygjG"
                     /locus_tag="KLK57_003822"
     CDS             2702..4081
                     /gene="ygjG"
                     /locus_tag="KLK57_003822"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HBE0894073.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGSLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMQLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQADQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(4123..4455)
                     /locus_tag="KLK57_003823"
     CDS             complement(4123..4455)
                     /locus_tag="KLK57_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HBE0894074.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            4674..5657
                     /gene="ebgR"
                     /locus_tag="KLK57_003824"
     CDS             4674..5657
                     /gene="ebgR"
                     /locus_tag="KLK57_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HBE0894075.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLFSVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
BASE COUNT         1387 a         1546 c         1435 g         1329 t
ORIGIN      
        1 tagatatatc taatcaagca aaaggaggct gatatgagcc atcatcacga agggtgttgt
       61 aaacatgaag gccagccacg ccatgagggc tgctgcaaag gtgagaagtc agaacacgag
      121 cactgcggac acggtcacca gcatgaacac ggtcaatgct gcggtggtcg ccacagtcgc
      181 ggcggcggtc gtcggcaacg tttctttggt cacggtgaat tgcgtctggt gattctggat
      241 attctctcgc gcgatgacag ccacggttac gaattgatta aagcgattga gaatctaacc
      301 caggggaatt acaccccaag cccgggcgtc atctacccaa cgctggattt tctgcaggag
      361 cagtcgctga ttaccatccg cgaagaggaa ggtggtaaga agcagattgc gctgaccgaa
      421 caaggcgcgc agtggctgga agaaaaccgc gaacaggtgg agatgattga agaacgcatc
      481 aaagcgcgtt gcgttggtgc tgcgctgcgc cagaacccgc aaatgaagcg ggcgctggat
      541 aattttaaag cggtgctgga tttacgcgtc aaccagagcg atatcactga tgcacaaata
      601 aaaaagatca ttgcggtgat cgaccacgcc gcttttgata ttacccaact ggattaatca
      661 ccgattgccg gatgcggcgt accctacaca ccccgcaggc ctgataagat gcgccagcat
      721 cgcatcaggc attgtgctcc aaccgccgga tccggcatac cgattaatgc agcaccgtca
      781 ccgcgtcttc cagtcggctg gcgcggtgtt tcaccatcgc cgacacctgc gcactctctt
      841 ccaccagctc agcatttttc tgggtgatca ggttaagctc atccactgca cgggtcaggc
      901 tggaaagccc atcggcctgt tccagcgttg aatggctaat ctgggcgatc aactgggtga
      961 cgtttttcac ctgtgccaca atatcttcca tcgtccgtcc ggcggcgtgt acctgctgcg
     1021 aaccggattg caccttatca gcactggcat caatcagctt gcgaatatcg ttggcagcat
     1081 tagcgctgcg gctggctaaa tgacgcactt cccctgccac cactgcaaaa cctttgccct
     1141 gttcgccggc acgcgccgct tccaccgccg cattcagggc cagaatattg gtctgaaacg
     1201 caatatcgtt aatcagcgaa gtaatggtgc caatgcgctg ggtactgtcg gcgatatcgt
     1261 ccattgtctt gatcaccgtg gtcatcgcct ccccaccctg cactgccgca ttactggcgg
     1321 tgatagaaag tttatcggca gccgacgccg tggcagagtt ctgtttcacc gacgccgcca
     1381 tttggttcat ggtcgccacc gtttgctgaa cgttatcaac tgtctgctgg gtatgttcgt
     1441 tcagttcatc ggtgcctttc gccagcgtct cactgccatt tctgacactg gacacctggc
     1501 ttgagacatc gttaattagc caacggcaca tcaggccaag ctgccctacc gcgcgtaatg
     1561 tcagccccag ctcatcgctg cgattcaggt gctcaacact attacgctct ccggtcgcca
     1621 ccttcagtgc ctgacgggcg acattttcta tcgggcgcac aatctgccat tcaaaacaag
     1681 cgcttgccaa caataccact aacacacaga ggaaatacgt caccaccggg gcagcaacaa
     1741 accaaagcat ggccgccagc aagataaaca tcagggtcat cactccacgc gcccgccagc
     1801 gaagcggtaa tgaaggcagt ttacccagcc agcctttacg caccaccagg cctttatgaa
     1861 tacgcttact ggtacgtccg gcgttcagcg ctttgtacag cggctccacc gccgcaatct
     1921 cttcatccgt cgcccgggta cgaatcgaca tatagccact gatttttccc tcgcgcacca
     1981 tcggtaccgc attggcccgc acccaataat ggtcgccatt tttgcggcga tttttcacga
     2041 tgccgctcca gggctcccct tttttcaggg tgaaccacat atccgcaaac gccgcttttg
     2101 gcatatccgg gtgacgcacc atgttgtgcg gctgcccttg taactcttgc aaggtaaagc
     2161 cgctcacctg cacaaaagtg tcattagcat gagtgatata gctttgcaga tcggtagtgg
     2221 acatcagagt ggtatcgtcc gccagcgggg tattttgctg ggtgacatac ggatgagaag
     2281 acatgttcgc gtcctgtgca ggttatatgg ttgttaactt cttgtcagag tttatgtcgg
     2341 ccccgctgcg gttatcttta accgattaat ttgatttaga tcgcaatttg cgattaaagc
     2401 acaaatctaa ttctttgatt taaaatactt tcactctgtt actatacgaa aacgttaatt
     2461 atcttgccca aaaatcaggc aattattgcc ctgaaaacgt gcatttgcgc agcaatcatc
     2521 aaatccatac ccgacaaaaa ccgtgcaaaa taacaacaaa tgttaacaga tagcattaaa
     2581 tattgcacaa atgataaccg aatttgtgtt tatcccgatt ttcgcgatcg cagccggagt
     2641 ggcgcaatcc ctgcaatact taaatcggta tcatgtgata cgcgagcctc cggagcatat
     2701 tttgaacagg ttaccttcga gcgcatcggc tttagcgtgc agcgcccacg ccctgaatct
     2761 cattgagaag cgaacgctgg atcatgagga gatgaaagca cttaaccgag aggtgattga
     2821 atacttcaaa gagcatgtca atccggggtt tttagagtat cgcaaatctg ttaccgccgg
     2881 cggggattac ggagccgtag agtggcaagc gggaagttta aatacgcttg tcgacaccca
     2941 gggacaggag tttatcgact gcctgggagg ttttggaatt ttcaacgtgg ggcaccgtaa
     3001 tccagttgtg gtttccgccg tacagaatca acttgcgaaa caaccgctgc acagccagga
     3061 gctgctcgat ccgttacggg cgatgttggc gaaaaccctt gctgcgctaa cgcccggtaa
     3121 actgaaatac agcttcttct gtaatagcgg caccgagtcc gttgaagcag cgctgaagct
     3181 ggcgaaagct taccagtcac cgcgcggcaa gtttactttt attgccacca gcggcgcgtt
     3241 ccacggtaaa tcacttggcg cgctgtcggc cacggcgaaa tcgaccttcc gcaaaccgtt
     3301 tatgcagtta ctgccgggct tccgtcatgt gccgtttggc aatatcgaag ccatgcgcac
     3361 ggctcttaac gagtgcaaaa aaaccggtga tgatgtggct gcggtgatcc tcgaaccgat
     3421 tcagggtgaa ggtggcgtaa ttctgccgcc gccgggctat ctcaccgccg tacgtaagct
     3481 atgcgatgag ttcggcgcac tgatgatcct cgatgaagta caaacgggca tggggcgcac
     3541 gggcaagatg ttcgcctgcg agcatgagaa cgtacagccg gatatcctct gccttgccaa
     3601 agcgctcggc ggcggcgtga tgccgattgg cgcgaccatc gccactgaag aggtgttttc
     3661 agttctgttc gacaacccat tcctgcatac caccaccttt ggcggcaacc cgctggcctg
     3721 tgcggcggcg ctggcgacca tcaatgtgtt gctggagcag aacttaccgg ctcaggctga
     3781 tcaaaaaggc gatatgttgc tggacggttt ccgtcaactg gcgcgggaat atcccgatct
     3841 ggtacaggaa gcgcgtggta aagggatgtt gatggcgatt gagtttgttg ataacgaaat
     3901 cggctataac tttgccagcg agatgttccg ccagcgcgta ctggtggccg gaacgctcaa
     3961 taacgccaaa acgatccgca ttgaaccgcc actgacactg accattgaac agtgtgaact
     4021 ggtgatcaaa gcggcgcgta aggcgctggc ggccatgcga gtaagtgtcg aagaagcgta
     4081 atatcaaatc ggatggcgat acgacgtcgc catccgatct ttttatacga cgcgtacgcc
     4141 cgcaggcatc atccgctccg gcgttaacag cacgctttca ctgccgtcat cggtttccgc
     4201 acacaacagc atgcattccg acgtttcacc acgcattttc gctttttgca gattgcataa
     4261 gaccaccacc gtttttccca tcagctcctc ttcgctgtag tacggcacca ggctggtcac
     4321 ggtttgcagt gttttttccc ccacatcaac ctgtacgatg tacagcttgt cggcgttttc
     4381 atggcgtttc acttccacaa tctttccgac gcgcatttcc agacgtgcaa aatcagcgta
     4441 agccacggtt tccattgctt cctcccttca agtaaaattt tactaaacta tagcaaaatt
     4501 ttttctcaat cctgtaggct aaaaatggag aatgcaggcg tgatcacatt cgtaagccgc
     4561 tgtgttaccg ttacagcgtc aaagaaacgc gctttattta ctgaaaacag gtgacccgat
     4621 aagcacttcc tctacaatgg gggcgcacat cagggaaagt aaaaaaggta aacatggcaa
     4681 cattaaaaga catcgcaatc gaagctggcg tatccctggc gacagtatcc agggtcttaa
     4741 atgacgatcc gacattgaat gtgaaagaag agacgaaaca tcgcattctc gagatcgccg
     4801 aaaagctgga gtacaagacc agtagcgccc gtaaactaca gacaggcgca gtcaaccaac
     4861 accatattct ggctatctac agctaccagc aggagctgga gatcaacgat ccttactatc
     4921 tggcgatccg ccacggcatt gaaactcagt gcgaaaagct ggcgatcgaa ctgaccaact
     4981 gttatgaaca caacggatta ccagacatta aaaacgtcac cggtatttta attgtcggca
     5041 aacccacgcc cgccctgcgc gccgctgcca gcgcgttgac cgacaatatc tgttttatcg
     5101 actttcacga acccggcagc ggttacgatg cggtggatat cgatctggca cgcatcagta
     5161 aagaaatcat cgacttctat atcaaccagg gcgttaatcg tattggtttt attggcggtg
     5221 aagatgagcc tggcaaggcg gatattcgtg aggtcgcctt tgcggaatat ggccgactga
     5281 aacaagtggt acgcgaagag gatatctggc gcggcggttt ttccagttcg tcgggttatg
     5341 aactggcaaa acaaatgctg gcgcgggaag actatccgaa ggcactgttt gttgcttccg
     5401 attccattgc tatcggcgta ctgcgggcaa ttcatgaacg tggcctgaac atcccacagg
     5461 atatttcgct tttcagcgtt aacgatatcc ccaccgcgcg atttaccttt ccgccgctct
     5521 ccaccgtgcg catccattcc gagatgatgg gaagtcaggg cgttaacctg gtgtatgaaa
     5581 aagcccgcga tggtcgcgcg ctgccgctgc tagtcttcgt tcccagcaaa ttaaaactgc
     5641 gcggcacgac ccgttaaatc cccttacaca ctgtccggca atcatttttg ccggaca
//
LOCUS       DAEEKQ010000213         5690 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.215, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000213 DAEEKQ010000000
VERSION     DAEEKQ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.215"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..116)
                     /locus_tag="KLK57_003825"
     CDS             complement(<1..116)
                     /locus_tag="KLK57_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HBE0894076.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLE"
     gene            complement(221..1723)
                     /gene="dtpA"
                     /locus_tag="KLK57_003826"
     CDS             complement(221..1723)
                     /gene="dtpA"
                     /locus_tag="KLK57_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HBE0894077.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(2334..2969)
                     /gene="nth"
                     /locus_tag="KLK57_003827"
     CDS             complement(2334..2969)
                     /gene="nth"
                     /locus_tag="KLK57_003827"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HBE0894078.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(2969..3664)
                     /gene="rsxE"
                     /locus_tag="KLK57_003828"
     CDS             complement(2969..3664)
                     /gene="rsxE"
                     /locus_tag="KLK57_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HBE0894079.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRTEAA
                     AERALPNGETGNV"
     gene            complement(3668..4288)
                     /gene="rsxG"
                     /locus_tag="KLK57_003829"
     CDS             complement(3668..4288)
                     /gene="rsxG"
                     /locus_tag="KLK57_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HBE0894080.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNTLAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(4292..5350)
                     /gene="rsxD"
                     /locus_tag="KLK57_003830"
     CDS             complement(4292..5350)
                     /gene="rsxD"
                     /locus_tag="KLK57_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HBE0894081.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSTLLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(5351..>5690)
                     /locus_tag="KLK57_003831"
     CDS             complement(5351..>5690)
                     /locus_tag="KLK57_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894082.1"
                     /translation="RAKARKLEQQQTNAEPEEQVDPRKAAVEAAIARAKARKLEQQQA
                     NAEPEEQVDPRKAAVEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKK
                     AAQQKVVNED"
BASE COUNT         1467 a         1447 c         1410 g         1366 t
ORIGIN      
        1 ttttcgagac gttttttcat taaatccaca ctgacgaggg taaaatcctt tccgctctca
       61 cgcagggtga tatgggaagc gagagagcag gcacccggtt tgtagaacaa tttcatcggt
      121 aactcctttt gtctgtggtt taaagtatgg tagtgcgttg ctgaacaaaa aaaagccgct
      181 aacgaaaagt tagcggcttg taaaagagtt tccctgaatt ttacgctaca gcagctttcg
      241 ccgctttgtc tgcagcgtca tcctgcgtca tacggtgcag tttaggcgcg gtcagcagca
      301 tcagtactgc aataatggca gtagcgacac caatctgcaa gaatacgcga ccataaactt
      361 ccagtgacat cagcgggtcg gtaacgttat ccggcacagc catcataccc gcaacataac
      421 caccaatcag gtttgcaccg gcagtggtca ggaaccagct acccataatg aagcccatca
      481 gacgctgcgg aacgagttgt gcaaccattg ccagacccag accagagatc atcagttccc
      541 cgatgctctg caggccatag cttgcgacca gccagcttac agacacgata ccagcgtcag
      601 acgcgaattt cgcacccagc ggcagaatca ggaacgcacc agaacacatc accatgccga
      661 ttgcaaactt ggttggcatc ggcagggtat cgcccatctt gttatagata gcggccagaa
      721 tcggactacc gatgatgatc cagaacgggt tcagtgcctg atactgctca ggttctacgg
      781 ccagacccag aatggagtgc tcaacgttac gaatcgcaaa gaagttcagt gacgttggca
      841 tctggctgta cagcacgaag aagataatgg cttcgagcat caggatgaag gcaacgatca
      901 ttttacgacg cgcagcacct ttcatcgcga aggcttcttt accgaagata accacgatac
      961 cgaaggcaac aacgcccagc gccatacgcg caacttcctg attgtgcagc agccaggtgg
     1021 cgatagcgat cagtgccaca acaccaataa tggtcagcag caggttacgg tagttgatag
     1081 gctcgaagtc tggttttgaa ccgtactgtt taacccagcg ttggcagaag gcgaagttaa
     1141 cgatagtgat cagcaggcct acaacgctca acgcaaacgc aacactccag ccgtatttcg
     1201 cggccagcca cggcgtagca atcatagaga agaaagagcc gatgttgacg gacatgtagt
     1261 acatggtgaa tgcaccgtcc agacgcgggt cgtttttctc atagcatgta gaaagcagag
     1321 aagatgggtt agctttaaac aggccgttac cgaccgcaat agccgccata cccatataaa
     1381 cgataccggc gtcgtgacca gaccaggcaa ccagagcata accaatcgcc agcacaatag
     1441 caccgagcat aattacgcgt ttagtaccca gtaccttgtc acctaaccag ccgccgatag
     1501 cgaccagacc ataaaccagg gcactaaagg aagagaaaag ggtgattgaa tccgcttcag
     1561 acatacccag ttgtttaacc aggtaaacag ccataattcc ttgtaggccg taataaccaa
     1621 aacgttccca taactcaatc gagaagatga gatagaacgc cttcggttgt ttgaaagcgt
     1681 tcaaactgac gctttcagtt ggtttttggt ttgcagtgga cacttttacc tcttttttta
     1741 catcccatat taacgggggt gctttctcgc gttatgtcta aaaaacatac gcgcaattgt
     1801 tatagtggga ggggaaacgg caggtaatgt tcactatctt gcactttcag acaagtcctt
     1861 tgtaataatc tgttacatat aactgaggcg gggaattcaa ccattccggg aaatgttatc
     1921 cagcgttaaa tttttcaaaa aacgtaatgg cagatttttt cactgcactg agaggggggt
     1981 gatggcttaa cggcgatatg ttctgctatt tagcgttttt actagtgaga tagtccagtt
     2041 tctgaaaaat agccagtgta atgttttgta ggtcaatatt tgttgcgtgt gtaaatttaa
     2101 attattttaa cctgattctt gcaatgtttt tacattgcga cagcatcgtg atcttgatca
     2161 cgaatgaata gaaaatttcg ctattaaaaa aacaattaac ctgcattttt ggttatccgc
     2221 aaacggatta cctgatgata acgctaagca atggcatcat tcacgttaaa aagatgtttt
     2281 aaaagaggat aaagaaaggt tatcaatggg gtaatcggtg ttaccccttt tcttcagatg
     2341 tcaactttct ctttgtattc acaaagatct tcaataatac aagagccaca gcggggcttg
     2401 cgggcaatgc aggtataacg cccgtgcagg atcaaccaat ggtggcagtc gactttaaac
     2461 tctgccggaa ccactttcag tagcttttct tctacctgtt cgacgttttt ccccggcgca
     2521 aattgagtac gattacaaac gcggaaaatg tgcgtgtcga cagcaatagt cggccagccg
     2581 aatgcagtgt ttaatacgac gttggctgtt ttacgaccta cgccgggcag ggcttcaagc
     2641 gcagcacgat cttccggaac ctcgccatta tgctgctcca gtaagatacg gcaggttttg
     2701 atgatatttt ctgctttgct gttataaagc ccaatcgttt tgatataggt tttaacccct
     2761 tcaacgccca gttcaagcat cgctgcaggc gtattcgcca ccgggtaaag tttcgccgtc
     2821 gccttattaa cactgacatc ggtcgcctga gcggaaagca gtacggcaat cagcaattca
     2881 aaaggcgaac tgaaattaag ctcggtggtg ggatgaggat tgttctcacg caggcgagtg
     2941 aggatctcca ggcgttttgc tttgttcatc agacattccc tgtttcaccg tttggcaatg
     3001 cacgttcggc agctgcttca gtacggcgct ttttcattct ttcatcaatc aggtattttc
     3061 ctgccagcat cagtcccagg ccaataaatg cacctggtgg cagcatcgcc agcaggaaag
     3121 gggagtcggt gtggaaaatc tccacgcgta atacttttgc ccagctacct aacagcgcat
     3181 ctgcaccgtc aaacaatgtg ccattgccga taatttcgcg tagtgaaccc agcacgaaca
     3241 tggcgcaggt tgcgcccata ccaattgaaa agccgtccag tgccgaaagc gccggacctt
     3301 ttttggcggc gaaggcttca gcgcggccca caacgataca gttagtgaca atcagcggaa
     3361 taaaaatccc taatgattga tacaggccaa aggcgtaggc gttgatcagc atctgtacag
     3421 cgctgaccac cgaggcgatg atcatcacgt aaatgggaat gcggatctcg gctggcgtcc
     3481 agtgacgcag cgtcgaaatg gtcaggttgg tcagcgtcag taccagcgta gtcgcaagtc
     3541 ctaaacccag agcgttagtg gcagtggacg tgaccgccaa cagaggacaa aggccgagca
     3601 actggaccag cgcagagttg tttttccaca acccctgaac aataacgtct ttaatttcgc
     3661 tcacgcttta ttctccacag gcaggaagtt gagaaagttg tgccggtaac gtctgagcgt
     3721 acaatccggc gcgttttacc gcattaacca ccgcgcgggg agtaatcgtc gcgccggtga
     3781 actggtcgaa atcaccacca tctttcttca ccgcccagtg cgcatcatcc gcaccactga
     3841 tttttttacc cgcaaaatgg gtgatccagt cagaaaggcg cagttcgatt ttatcgccaa
     3901 gccctggcgt ttcgtggtgc tctgtcacgc gcgtgccaag taccgtgccg ttaaaatcgg
     3961 ctcccaccag cagctgaatc gcaccggaat agccatctgg cgcggttgct tccagaacgg
     4021 cggctaccgg tttgtcatcc tgtttggcga tgtaaacccg atgctcacct ttacctaact
     4081 ctggcgcagt taccagatag caactctgtg ccagcgtatt gttatagcgt tcggctggca
     4141 gcacctgatc aaataatgcc ttttgttgca gactggcctg ttcagcaatc gtcgttttgg
     4201 tcatctggtt gatggccgca gttaaccctg ttgaacccgc tgcaaatagc gctaacgtaa
     4261 tgccgtgttt tcggatagtt ttcagcatgg tttacccttt gcgatggccg tagacgcgcg
     4321 gacgcgtgta gtaatcgatc agaggaaccg tgatgttcgc cagcaggacg gcaaaagcca
     4381 cgccgtcagg atagccgccg aaactgcgga tcaaccagac taataagccc gcaagcgcgc
     4441 cgaaaataag acgaccacga ttggtcgtag aagcggtaac cgggtcagtc aaaataaaga
     4501 atgcgccgag catggtcgca ccagacagca gatgaatttg cggtgctgcc agtgtttctg
     4561 gtgagaacaa ccagcccaac gttgcgcata acgccagcgt tactaagaag ctgaggggaa
     4621 tatgccagcg aatcgctttc tgccatagca accacaggcc gccagccagc caggcgagat
     4681 ttacccattg ccaaccagcg cccgccagaa taccgctgta gatcggatat tgcataatct
     4741 gttcaaccga atgaccggca cggacagagg ttttaaatgt atccagcggt gtcgcctgac
     4801 taatgccatc aatacctaag cgtagtgtgt tcatatcacc accactggcg gtatgcccgc
     4861 tgaaaataac ctgtatggcg tcgataaaac cagggatgtt gaccgcaatt tcatgtggcg
     4921 gtaaccagct ggtcatctgc accgggaagg agatcagtaa gaccacataa ccaatcattg
     4981 ccggattaaa cgggttttgc cccagaccgc catacaactg tttagcgatg atcaccgcaa
     5041 acaccgtacc cagcacgacc atccaccatg gcgcgagggg gggaatactt accgccagca
     5101 ataagcctgt cagcaatgct gagttatctt tcaacgttgc ggctaccgac tgcttgcgta
     5161 gtttgagtac gagagcttcg gctaacagag tactaaccga tgccaacagg atctgaacga
     5221 gagtacccca accaaaaaac cacagttgcg ctgcgattcc tggcacggct gcgagcaaca
     5281 ccaacagcat aatgcgcgat gtctggcgct ggttatgggt ataaggggag ctagctattc
     5341 tgaataccat ttagtcctcg tttacaacct tctgctgggc ggcttttttg gcctgagcgc
     5401 gggcaatagc cgcggcaact gccgctttgc gcggatcaac ctgttcttct ggttctgcgt
     5461 tagtctgttg ctgttccagc ttgcgcgctt tggcacgggc aatagcggct tcgacggcgg
     5521 ctttgcgcgg atcaacctgt tcttctggtt ccgcattagc ctgttgctgt tccagtttgc
     5581 gcgctttggc tcgggcaata gcggcttcga cggcggcttt gcgcggatca acctgttctt
     5641 ctggttctgc gttagtctgt tgctgttcca gcttgcgcgc tttggcacgg
//
LOCUS       DAEEKQ010000214         5640 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.216, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000214 DAEEKQ010000000
VERSION     DAEEKQ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5640
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.216"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(33..770)
                     /gene="bamD"
                     /locus_tag="KLK57_003832"
     CDS             complement(33..770)
                     /gene="bamD"
                     /locus_tag="KLK57_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HBE0894083.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            905..1885
                     /gene="rluD"
                     /locus_tag="KLK57_003833"
     CDS             905..1885
                     /gene="rluD"
                     /locus_tag="KLK57_003833"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HBE0894084.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1882..2613
                     /gene="pgeF"
                     /locus_tag="KLK57_003834"
     CDS             1882..2613
                     /gene="pgeF"
                     /locus_tag="KLK57_003834"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HBE0894085.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2743..5316
                     /gene="clpB"
                     /locus_tag="KLK57_003835"
     CDS             2743..5316
                     /gene="clpB"
                     /locus_tag="KLK57_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HBE0894086.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1401 a         1351 c         1529 g         1359 t
ORIGIN      
        1 caagggctgc cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg
       61 attttcgcta ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc
      121 agtggcagcg catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctacg
      181 cggttaacga cggcaaccca tgcaccacgt tctgtatagt actcggcaac ggagtattca
      241 tatttcgcca gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga
      301 ctgttcggat agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga
      361 ggatcgcgat cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc
      421 atattggtca ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg
      481 cgaataaaac gatcgatggc agcctgtgct aacggcaaat cggcgttttt atagtaggcg
      541 tagatgagat ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac
      601 gcttccagtt gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc
      661 gcgtaaattt catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc
      721 aaaaacaggc ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga
      781 ctttcctcaa aatgtaatac gggagattct ctgttcctgc tcccggttaa gaccagctac
      841 aatagcacac tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt
      901 atatatggca caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg
      961 cttagatcag gctttggccg aaatgttccc ggattattca cgttcgcgga taaaagaatg
     1021 gatcctcgac cagcgcgtgc tggttaacgg caaagtttgt gataagccga aagaaaaagt
     1081 attgggtggc gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc
     1141 gcaggatatc ccgctggata tcgtctatga agatgaagat atcattgtca ttaataaacc
     1201 gcgcgaccta gtagtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt
     1261 gcttcattat tatccgccca ttgccgatgt accgcgtgcg ggcatcgtcc atcgtctgga
     1321 taaagacacc accggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt
     1381 cgaatctttg caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat
     1441 gaccgctggc ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat
     1501 ggcggtgcat ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg
     1561 tgtgcacacg cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca
     1621 tatggcccat atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc
     1681 gccaaaaggt gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct
     1741 tcatgcaacc atgctgcgtc tttatcaccc gatctctggc atcgaaatgg aatggcatgc
     1801 gcctattcca caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca
     1861 taaggatgaa gtggactggt tatgagtaag ctgattgtcc cgcagtggcc actgccaaaa
     1921 ggtgttgcgg cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca
     1981 ctcaacctcg gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgtaagcga
     2041 ctttttgctg cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa
     2101 gatgtgctta agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc
     2161 aatacgcctg gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc
     2221 aatcgagcgg gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc
     2281 gtgctggaag agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta
     2341 gggccggcaa ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg
     2401 gcagtagacg ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat
     2461 atttatcagc ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc
     2521 gaccgttgta catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc
     2581 ggtcgtatgg caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg
     2641 cgtgattttc ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca
     2701 tttaatctcc agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt
     2761 actaataaat tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac
     2821 caattcatcg aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt
     2881 agtcctttat taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag
     2941 gcattaaatc gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat
     3001 ctggtgcgcg ttcttaatct ttgcgacaag ctggcgcaaa aacgaggtga taactttatc
     3061 tcgtcagaac tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg
     3121 aaagcagcag gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt
     3181 gaaagcgtga acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc
     3241 gaccttaccg aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa
     3301 attcgccgta ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt
     3361 gaacccggcg tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc
     3421 gaagtgccgg aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg
     3481 gctggggcga aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt
     3541 gccaaacagg aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg
     3601 ggtaaagccg atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt
     3661 gaattgcact gcgtaggtgc cacgacgctt gacgaatatc gccagtacat tgaaaaagat
     3721 gctgcgctgg aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc
     3781 attgcgattc tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact
     3841 gacccggcaa ttgttgcagc ggcgacgttg tctcatcgct acattgctga ccgtcagctg
     3901 ccggataaag ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac
     3961 tcaaaaccag aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa
     4021 caggcgttaa tgaaagagtc tgatgaagcc agtaaaaaac gcttggatat gctcaacgaa
     4081 gaactgagcg acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag
     4141 gcatcgcttt ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt
     4201 gaacaggctc gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc
     4261 ccggaactgg aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg
     4321 ttgcgtaata aagtgaccga cgccgaaatt gctgaagtgc tggcgcgttg gacggggatt
     4381 ccggtttctc gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg
     4441 caccatcgcg tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt
     4501 agccgtgcgg ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca
     4561 acgggtgtgg ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac
     4621 gaggcgatgg tccgtatcga tatgtccgag tttatggaga aacactcggt gtctcgtctg
     4681 gttggtgcgc ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg
     4741 cgtcgtcgtc cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc
     4801 ttcaacattc tgttgcaggt actggatgat ggtcgtctga ctgacgggca ggggagaacg
     4861 gtcgacttcc gtaatacggt tgtcattatg acctctaacc tcggttccga tttgattcag
     4921 gaacgcttcg gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggtaagc
     4981 cataacttcc gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg
     5041 ggtgaacagc acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg
     5101 gaagaacgtg gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac
     5161 ggttacgatc cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa
     5221 aacccgctgg cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg
     5281 gaagttaatg aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggggct
     5341 cgtttttgtc tataagttag acggaaaaga ctatatttaa gatgttttgc ctgaaaaatg
     5401 agcgaacgat aaagttttta tatttttcgc ttgtcaggcc ggaataactc cctataatgc
     5461 gccaccactg acacggaaca acggcaaaca cgccgccggg tcggcggggt tctcctgaga
     5521 attccggcag agaaagcaaa aataaatgct tgactctgta gcgggaaagc gtattatgca
     5581 caccccgcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt atcagacaat
//
LOCUS       DAEEKQ010000215         5604 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.217, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000215 DAEEKQ010000000
VERSION     DAEEKQ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.217"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..513)
                     /locus_tag="KLK57_003836"
     CDS             complement(<1..513)
                     /locus_tag="KLK57_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HBE0894087.1"
                     /translation="MGKYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIIDAELFALAGLDA
                     QHLQLLVHSGS"
     gene            complement(759..1211)
                     /locus_tag="KLK57_003837"
     CDS             complement(759..1211)
                     /locus_tag="KLK57_003837"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0894088.1"
                     /translation="MNNIQIRNYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVWVAVINAQPVGFISRIEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQHVECRGAWFTNFYMQYKPQH"
     gene            complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="KLK57_003838"
     CDS             complement(1208..2263)
                     /gene="dinB"
                     /locus_tag="KLK57_003838"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HBE0894089.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVMLLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="KLK57_003839"
     CDS             complement(2334..3119)
                     /gene="lafU"
                     /locus_tag="KLK57_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HBE0894090.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            3091..4803
                     /locus_tag="KLK57_003840"
                     /pseudo
     CDS             3091..4803
                     /locus_tag="KLK57_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="KLK57_003841"
     CDS             complement(4926..5423)
                     /gene="rayT"
                     /locus_tag="KLK57_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HBE0894091.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSQLLTTQYQMLRNAIIKVKR
                     DRPFEINAWVVLPEHMHCIWTLPEGDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPVKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDVTDINAG
                     ERIIL"
     gene            complement(5494..>5604)
                     /locus_tag="KLK57_003842"
     CDS             complement(5494..>5604)
                     /locus_tag="KLK57_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0894092.1"
                     /translation="ILTNDKVFKAGLRRKMRKAAMDRNYLASVLEGSGLS"
BASE COUNT         1334 a         1466 c         1456 g         1348 t
ORIGIN      
        1 cgagccgcta tgaaccaaca gttgcagatg ttgtgcatcc agacctgcca gtgcaaacag
       61 ttcagcgtcg atgatttgat caacttgttg cagttcagcg aaatgattgc cgccaccgat
      121 agagccaagc gagctgcgcc acggatgctg tgcaaatgct gacggcaggt tttcctccag
      181 ccagctttct tcagcaacgt catccaaagc tgataatcgc ttttcaaact tatcggcgtt
      241 gtatttgcga gcgagaatat ctgtttgcca tagcgccatg ccgcagccga tatcattgcc
      301 gaccagtgct gggtaaaaac ggccaacaga gaagaacgct gcgccaatcg ggtagccgcg
      361 tccgggatgt aaatctggca tccccactac gcgctgcatg ttgggtaaat ttgccgtggt
      421 gtgtaattgt tggatcgcta aactctcgat ccacaggtca tcagatgcga tggtaaatac
      481 cgcatcggat aagggacgaa tatatttgcc catgtaccat tccgtaaata gactcgtcat
      541 acttcaagtt gcatgtgctg cgactcgaat tattgagagt aacaattaaa aaatcaggaa
      601 atggcagacg aatccagggc aagaaaaaaa gaattaatcc tggaaaggtc tgcaaagagt
      661 cattgataaa ggatttgtca tgatttacct ccttgcagtt tgtggaataa gaaaacgcgc
      721 acatactagg cgggcgttat tttcattgca agctggattt aatgttgcgg tttatattgc
      781 atataaaaat tagtaaacca cgctccccgg cattcaacgt gctgctgctt aactgtctga
      841 aaaccataac gttcaaaaaa gggttttgcg gttatgctgg catctaccgt aagttcggat
      901 tcagacttaa taaatggttt taacaaggcg ctggcaaccc cgcggcgggt gtattcaggg
      961 tcaacaaata acatatcgat ataatgttca atgcgggaaa taaaaccaac cggttgtgca
     1021 ttaatgaccg caacccacac ttgtgatttc gcgagtttct ccttccagcg agattcgtca
     1081 atctgcgccc aggcggcaat ttgttgtgga gaataatgct gactggcggt catcgtaacc
     1141 gctctaatga aaatagcgca tagttgctga aaatcgccag gctgatagtt tcttatttgt
     1201 atgttattca taatcccagc accagttgtc tttccatttg cggatcaagc aacgtcacat
     1261 gcagccccac cagtcgcaca ccgcgcccgc cgcggcgttc atcccaggtt ttacgcgcgg
     1321 tggcgattaa gtcagcttta ttcagccgcg gccagacgtg ctcctgagtg gtttgctgaa
     1381 aatcgtcgaa ctttaatttc accccctggc gggcaatcag taaatcaggt tttacctttg
     1441 ccagacggcg ttcaagttcc ggatacagcc gctcgataat cgcttcacat tcagaccagt
     1501 ggtggatatc ttccgccatc gtgcgttcca cgccgacgga ttttcgcaac cgttcgctgt
     1561 taacgtcgcg ctcgtcaatc ccctgactac gctcccacaa aatgcggcca aatttgccaa
     1621 agcgtttaag cagcatcacc agatcacact tttgtacatc accgcaggtt cgtagcccca
     1681 tcgcttccag ttttgccgcc gagactttgc cgacgccggg gatttttgcc agtggtaagg
     1741 tttgtaaaaa tgccggaact tctgccggcg taatcacaaa ctggccgttg ggtttattca
     1801 tgtcggaggc gattttggcg agaaacttta cgggtgccac gcccgcagac gccgtcagtt
     1861 gcagctcgtt gaaaatcgtc tggcggattt cctgggcgat gagggtcgca gaaccgtggc
     1921 aatggacgct atcggtgacg tcgagataag cctcatccag tgacaacggt tcaatacgcg
     1981 aggtgtagcg cgagaagatt tcgcggatat gatttgaggc ttctttgtag gcgtcaaagc
     2041 gccccggaag caaggtgaga tgcgggcata atttgagcgc catccctgtc ggcatagcgc
     2101 tacgtacgcc aaatttacgc gcgggataat tggcggtact gatcaccccc cgacgttcgc
     2161 ggctgccgcc aatagcaata gggatatcgc gcagggcggg attgtcgcgc atctccaccg
     2221 ctgcgaaaaa gcagtccata tccacatgaa tgattttacg cattgctcac ctctcaacac
     2281 tggtaaagta tacagtgatt tcagagtttg agaaatgcgt aaagattcag catttaacgc
     2341 gttcgctgcg aaagcacctg ctgcttatcc agcttttgca ccagcggctg caccacttta
     2401 tccccatgct gaccaaagta ttgatacagc gtatcggacg cacttttggt cagcaccata
     2461 atctcaatgc gccggttgcc cgcgctttgc ggatttttgg aatccagcag catctggtcc
     2521 gccattgcgc ttacctgcat cactttatct tccggcattc cggcctcttc cagcacccga
     2581 cgagccgaaa gcgcgcgatc gcccgaaagg ttccagttgt tgtagatatt gtttttgtaa
     2641 gccatcgcat cggtatgccc ggtaataata attttgttat cgagcgagtc gaaaactggc
     2701 gcgagctcca ccagcagcgt tttaaagaac ggcataatct gcgcgctgcc gcgttcgaac
     2761 atattgcgat tctggtcgtc tttaatcagc acgcgtaatc cctggggaac aatctccatc
     2821 tccagattcg cttccatatg cgcatcacgg gcaatggtgg tgatactggt tgccagctcg
     2881 ccaagctcgg tggcggattt cttcttcagc agcgccgttt tctcattgac gtcgcgcgct
     2941 tttttctccg tttcttccgg caccgccacg gtggcaggtt ttggcggatg agacgggctg
     3001 attttattta acggcgacag cccgccaccg ttaaaaatcg actgcccgtg cagcgcggcg
     3061 ataatgcttt cacgctcgga tttactgacg ctgttgacaa tcaactttat tgtcgtcacc
     3121 aaaggggccg agcgtatctc cgaggtttct gcccgcttta ccctggacgc gatgcccggt
     3181 aagcagatgg cgattgacgc cgatcttaat gccgggttga tcaatcaggc gcaggcacaa
     3241 acccgacgta aagatgttgc cagcgaggcc gatttctacg gcgcgatgga cggggcatcg
     3301 aaatttgtgc gcggggatgc tatcgccggg atgatgattc tggcgatcaa cctgatcggc
     3361 ggcgtctgta tcgggatctt caaatacaac ctgagcgccg acgccgcctt ccagcagtat
     3421 gtgctgatga ccatcggcga cgggctggtg gcgcaaatcc cttccctgct actctccacc
     3481 gcggcggcga ttatcgtcac ccgcgtcagc gacaacggcg atatcgccca tgacgtgcgt
     3541 aaccagttgc tggcaagccc atcggtgctc tacaccgcta ccggaattat gttcgtactg
     3601 gcggtagtgc cgggaatgcc acatctgccg tttttgctgt tcagcgccct gcttggcttt
     3661 accggctggc ggatgagcaa acgcccgcag gcggcggaag cggaagagaa aagtctcgaa
     3721 acgctgaccc gcaccatcac cgaaaccagc gagcaacagg tcagttggga aaccattccg
     3781 ctgatcgagc ccatcagctt aagcctcggc tacaagctgg tggcgctggt ggacaaagcc
     3841 cagggcaacc cgctcaccca gcgtattcgc ggcgtgcgac aggtgatatc cgacggcaac
     3901 ggcgtgctgc tgccggagat ccgcattcgg gaaaacttcc gccttaagcc cagccagtac
     3961 gctattttca tcaacggcat taaggctgat gaagcggata ttccggcgga taaactgatg
     4021 gcgctgccct ccagcgaaac ctacggcgag atagacggcg tgctggggaa cgacccggcg
     4081 tacgggatgc cggtgacctg gatccagcct gcgcagaaag cgaaggcgct gaatatgggg
     4141 tatcaggtga tcgacagcgc cagcgtgatt gctacccatg taaataagat tgtgcgcagc
     4201 tatattcctg atttatttaa ctatgacgat attacgcagt tgcataaccg tttgtcgtcg
     4261 atggcaccgc gcctggcgga ggatttaagc gcggtgctca attacagcca gttgctgaaa
     4321 gtgtaccggg cgctgctgac cgaaggcgtt tccctgcgcg atatcgtcac catcgccacc
     4381 gtgctggtcg ccagtagcgc ggtgaccaaa gatcatattc tgctggcagc cgatgtgcgc
     4441 ctggcgctgc ggcgcagcat tacccatccg ttcgttcgca agcaggagct gacggtgtat
     4501 accctgaata atgagctgga aaatctgctg actaacctag tgaatcaggc gcaacagggc
     4561 ggtaaagtga tgctcgacag cgtgccagtg gacccgaata tgctcaacca gttccagagc
     4621 acgatgccac aggtgaaaga gcagatgaaa gcggcgggga aagacccggt gctgctggtg
     4681 ccaccgcagc tgcgcccttt gctggcgcgt tatgcaaggt tgtttgcgcc ggggctgcat
     4741 gtgctgtcgt ataacgaagt gccggacgag ctggagttga agattatggg ggcgttgatg
     4801 taagtatccg gcattggtgc acaactgccg gatgcggcgt aaacgcctta tccggcctac
     4861 cgttcaggca caagtttgta ggcatgataa gacgcgccag cgtcgcatca ggcatctgcg
     4921 cataattata aaatgatacg ctcgccagca tttatatccg ttacgtcccc cgcccaatcg
     4981 atgggatata accctcgcgc gacatcgcga tggaacgttg agaatggcca atcactcact
     5041 tgctttaccc aaccatgctt tactggattt atataaatat aatcaacatg atgcctataa
     5101 tctttggtat tgcggatggc gtgctcccaa aaacgtggtt gccagatatt tttcaatcca
     5161 caagcatggg taaattgctt tttaatttcc cgccagcgag aggaaaaatc atcatcgcct
     5221 tcaggtaatg tccatatgca atgcatatgc tctggcaaaa cgacccaggc gttgatttca
     5281 aaaggcctgt ctcgcttaac tttaataatg gcgttacgga gcatctggta ctgggtggtc
     5341 aagagttggc ttcgacgatt gcgtaaattc accgtgaaaa accaggttcc ccccttgatg
     5401 taataacgac gatattcaga catggcgact cctttttctc aacggtaaga agaaaaggaa
     5461 aggtcggcaa tgaggtgatg gaacagggca agattacgaa agcccgctcc cctcaaggac
     5521 tgacgccaga tagtttctgt ccatggctgc ttttcgcatc ttacgtctta accctgcctt
     5581 gaatacctta tcattcgtca aaat
//
LOCUS       DAEEKQ010000216         5569 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.218, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000216 DAEEKQ010000000
VERSION     DAEEKQ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.218"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            313..882
                     /locus_tag="KLK57_003843"
     CDS             313..882
                     /locus_tag="KLK57_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0894093.1"
                     /translation="MRTFLYARVSVDDLTTQNQVEAVKRAGYEVRPSRVIEEVISGAT
                     PAMDRPQFVKLVDKLEEGDCLVVTKIDRLGRDNIDVQKTVTMLLGMGVKLVCLDLPVQ
                     DLSSAEGKLILQLFSTFAEFELNRIRERTREGLERAKAQGKVLGRPVAKNTTEAVLEH
                     KAKGLSQSQVAKLLNLSVRTVSRHWTKTL"
     gene            1611..1784
                     /locus_tag="KLK57_003844"
     CDS             1611..1784
                     /locus_tag="KLK57_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HBE0894094.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            complement(2042..3370)
                     /gene="rutG"
                     /locus_tag="KLK57_003845"
     CDS             complement(2042..3370)
                     /gene="rutG"
                     /locus_tag="KLK57_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HBE0894095.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNSQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(3391..3885)
                     /gene="rutF"
                     /locus_tag="KLK57_003846"
     CDS             complement(3391..3885)
                     /gene="rutF"
                     /locus_tag="KLK57_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HBE0894096.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPSGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            complement(3896..4486)
                     /locus_tag="KLK57_003847"
     CDS             complement(3896..4486)
                     /locus_tag="KLK57_003847"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HBE0894097.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYAPIKWGP
                     TSANCSPARIVFIRTVEGKERLHPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            complement(4496..5296)
                     /gene="rutD"
                     /locus_tag="KLK57_003848"
     CDS             complement(4496..5296)
                     /gene="rutD"
                     /locus_tag="KLK57_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HBE0894098.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALIRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            complement(5304..>5569)
                     /locus_tag="KLK57_003849"
     CDS             complement(5304..>5569)
                     /locus_tag="KLK57_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBE0894099.1"
                     /translation="VLFADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKN
                     YAAINEIYAEFFPGDKPARFCIQCGLVKPDALVEIATIAHIAK"
BASE COUNT         1470 a         1462 c         1387 g         1250 t
ORIGIN      
        1 gtagcccctc ttcaaattcc ccttctaaaa atcttcgaaa atatctgaca gatgtctgta
       61 gctgctaagt acccgtcaca ccagctatgt gacccccttt gcaaaaaagc caaatatcat
      121 tgactttctc ggtgaattgc tataaaggca tctatctagg tctttaactg tataaataca
      181 catgacaaca aacgcccgtt aagtgtctat ataaaaaggt agctgtagct atagacaaca
      241 attgtgataa ttgatttaat ggctatagac aagcaagaca gacctttgtt gactatatag
      301 agagataaga acatgcgtac attcctttat gctcgtgtat cagttgatga cctcaccaca
      361 cagaaccaag tagaagctgt taaacgtgct ggttatgaag tcagaccttc acgagtaatt
      421 gaggaagtta ttagtggggc tacaccagct atggacagac cacaatttgt aaagctggtg
      481 gataagctgg aagaaggtga ttgtcttgta gtgaccaaga ttgaccgctt aggaagggat
      541 aacattgacg tacagaagac cgtcacaatg ttacttggta tgggtgttaa gctggtctgt
      601 ctagatttgc ctgtccaaga cctttcaagt gctgaaggta aacttattct tcaactattc
      661 agtacctttg cagagtttga acttaaccgc atcagagagc gaaccagaga gggcttagaa
      721 cgagctaaag cacaaggtaa ggtgttgggt cgtcctgtag ctaaaaacac tacagaggcc
      781 gttttagagc ataaggctaa gggattgtca caaagtcagg ttgcaaagct tctgaatctc
      841 tctgtgagaa cggttagccg tcactggaca aagacactgt aaaaaaaact tagtagcatc
      901 ttcatagcct ccttcatggg gacttttttg tgtctgtaac atatggtgac caaacatcta
      961 tatatcgtta tatagccctg tatatagcac tatatatagt gtctggagag ggacgactaa
     1021 caccctgcat agttccctga aaagggggct tcacagattc tctatatatt tcaataagtt
     1081 aggataaaaa gggactatac cgatactgca tagaatctgt gtaggattgc atagtcatag
     1141 gggttggaac aagcccaact acacctgata aagcctgtta ttaacaggta gttgaatgta
     1201 cgtgtttagc tatgtaaaag caccagaact ttagccattt ctaaccactc ctcgtgttat
     1261 ctctattccg tagcgattcg ctaccactta tttaaagata agacgtcctt ttcagagtgc
     1321 aaatttcaca accacttatt tgatttataa caactttcac aagcacgtaa ttttgtcgca
     1381 aaatgacaca tttttatctc atcgcgtttt tttaatcata agagcggctt atggataatt
     1441 attggagatg atatctattc tcgctaagaa gctgttgcag gatattacca aacgcgggtc
     1501 tacccgcgtc agttcactaa gcttagtccc acgtagcgaa aatatggcag ccgccatacg
     1561 ccgcgttaat tctatgcaat atgatgtcta tacccagacg gaggtcagta atggcaaacc
     1621 atcgaggcgg ttccggcaat tttgcagaag accgcgaaag agcatcagaa gcaggtaaaa
     1681 aaggtggaca gcacagcggg ggtaatttca agaatgaccc gcagcgcgca tctgaagcag
     1741 gtaaaaaagg tggcaagagc agtcacggca aaagcgacaa ctagccgggc taatcaatga
     1801 cgaatgcatt tttgtctgta gctcgtcaaa aagccatcac cgccggttac ccggtggttg
     1861 atactgatga caaatgtaag cttgcctgat gcgcgatgct tatcaggcct accagaagat
     1921 tgcaatatat tgaatttgca ctgttttgta ggccggataa ggcgtttacg ccgcatccgg
     1981 catgaacaat gcgtacgttg tcaacaatct gcaccgccgg gtaaccccgg cggttttctg
     2041 tttatggctc ctgatgaaca acttctggcg gtggaacgtc aaccaacctt ctacttaaca
     2101 acgcattgag taaaatcgcg ccaaaggtgg ctgtaccgat ccctcccaac gtaaaaccgc
     2161 ccagcgtgag agcaaaatca cccgcgccca gcactaaggt tactgcgacc ataatcaaat
     2221 taccgttctg gcttaaatcg acacggtttt gtacccagat ccttgcgcct gcgacggcaa
     2281 tcagcccgaa cacaacaatt gatgcaccac caatcaccgc ggccggaatg gtatggatca
     2341 gcgcgccaaa tttcggcgaa aagcccaaca gcatggcgat gacggcagca gcaacaaaca
     2401 ccagcgtcga gtagactttt gtcacggcca tcacaccgat attttcagca taggtggtga
     2461 cgccgctacc gccgacagag ccggaaagca tcgttgccag accatcaccg acgaatgctc
     2521 gccccatata cgggtccata ttgcgtccgg tcatcccggc gaccgccttg agatgaccta
     2581 agttttccgc caccagaatc accgccacgg gcgcaatcag catcattgcc tgactattaa
     2641 aagcaggagt ggaaaaatgt ggcaaaccga accaggcagc atggctgacg agagtaaaat
     2701 cgacggcttt tcccagccct aaaacgttgg tcatcacgcc atacagcaga caggcgacaa
     2761 ttaaacctac gagaatcaat aaccgctgga tcatgccacg ggtaaacacc gccaccagcc
     2821 caatacacag caccgtcatt accgccatcc agctatcaaa ggctgaagcc gatacacttt
     2881 tcactgcgat aggcgctaag ttcaggccaa tcgccatcac caccgcaccc gtcaccaccg
     2941 gcggcattag tcgttcaatc cagcgtgtac caattttcat caccaccagg ccaatgacgg
     3001 tataaaccag cccacaggcg ataatcccgc ccagtgcgat gctgatattc gggttaatgc
     3061 cctgaccgtt aaagcccgtc gcggcaatca ccacgccgac aaaagccgcg ctggagccga
     3121 gataactggg gacgcgcccg ccggtaataa agaaaaacag taacgtgccg atccccgaca
     3181 ttaaaatgga aagattggga tccagcccca tcagaatcgg cattaacacc gtcgcgccaa
     3241 acatcgccac cgcgtgttga acgcccatta ctgccgtctg agcaaacggc aatcgttcat
     3301 ccggcgcgac cacgccgctc tctgtagagg tcgattttaa ctgccagtga ggaaaaccga
     3361 acattgccat cagctgtctc cttaaggagg ttaacaagca gggcgcatca gcgcgtgata
     3421 actgcgatcg aaccacacca gcccgtaggg tgtggtgtga cgatgaatcg cttcgatggc
     3481 gcaaaacaga atgtcgtggg tgccgacgct caccacctgg ctgatgcggc agtcaaacga
     3541 aaccagagcc tcttccagtt gcgggcatcc ggtcacaccc gtctgccagc gggcggcggc
     3601 aaagcggtgt tccatgggcg ttttgccgcc aaaaagattt gaaagcggct cctgcccggc
     3661 gctcagtgta tttacacaca gcgttcgatt ttcattgaat accggccaga cggacgcccc
     3721 acgattcagg cacaccagta atgtgggcgg cgtgtcggtc acgctgcaga cggcgctggc
     3781 ggtgaacccg gcgcgcccgg atggaccgtc cgtggtgatg atattgaccg ccgcgcccat
     3841 acaggacatc gcatcgcgaa aggtttgttg atcaactatg ttcatagttt gctccttaca
     3901 acagcccgca ggcttcttca aaggacagac gtggcaggcg cgcaaaaagt ttgctgctat
     3961 cgccatagcc gatattaatc agcagattgc tcttcagcgt gctgcccgca aaaaaggcgt
     4021 cgtcaacgtg ttgacggtca aaacccgaca tcgggccggt atccagtccc agcgcacggc
     4081 aagcgacgat cagataggcc gcctgcatgg agctgttgcg aaacgctgtt tcttcggcaa
     4141 gttgtgggct ggaggtaaac caactgcggg catcaccgtg gggaaacagt agtggtaacc
     4201 gttcataaaa ttcactgtcc caggcaacga tagcggtgac gggcgcggtc agggtttttt
     4261 gtagattgcc gctggaaagc gccgggtgca gacgttcttt tccttctaca gtgcggataa
     4321 acacgatccg tgccggagaa cagttagctg atgtcggccc ccattttatc ggggcataaa
     4381 gctcccgtaa cgtctcatcg ctgacgggtg tctcccgcca gccgttgtga gtgcgggcat
     4441 cggtgaacag ggtgctaagc gcacctgggc taacggcttc gttcatagca attccttaca
     4501 gggcggcttc acggtgatgt aacaggctgg caagcccgtt gagtaacaga gcattaaacg
     4561 tttcggggtc ggtcacgttg caggcgtgtc cgccatagcg catcaccatt ttctggctat
     4621 cgggcagggc ggcatgaagc tcactggaac atgctgatgg caccagcaga tcatcactgg
     4681 cgcagatgat ttgcaccggg cagcggatgc gatccgcatg gtgactaaag tcagcgcgta
     4741 tgagggcgtt aagtcgacgc agtaaattat ttttgccctg aaaatgcgcc agtgccagcg
     4801 cgtcttctgc ctcaaggcga ggtgcgcggg ccgccatcca gtcggcggga tagaggaaca
     4861 acggctgcgc ttccacccat gcctgcgcgc cgccgctata cagtaatcgt tcgcgaacct
     4921 gaaaacagcg gcgcgtatgg gcgtttattc gtagccagcc gttaacgctg atcagcacag
     4981 ttaccgacgc gggataatcc agcgccagct gcattcccac cagcgcaccg agcgcatggc
     5041 cgaccaccgc gtaatgctca atccctgcgg ctaccagcgc ctgatgcagt tccgctgcca
     5101 tctgggcgat actgtaatct tctgccagcg tgtcgggatt attgccggtg ccgcgctggt
     5161 cgtaacagac tacctgatac tcctgctcca gcaccgccag ttgcggtaac cagtaactgc
     5221 cgctaccgcc aagacccgaa atcaacacca ctacgggcgc atcagcataa gggggaggtg
     5281 agagtgaaag tttcatcgcg gcctcacttg gcgatatgcg caattgtggc gatttccacc
     5341 agcgcgtcag gttttaccag tccgcactga atgcagaatc gcgccggttt atcacccgga
     5401 aaaaactcgg cgtagatttc gttaatcgcg gcgtaatttt tccagtcggt aataaagatg
     5461 ctgttgaagg tcacatccgc catcgtgcca cccgccgtct cgatcacctt gcggatagtt
     5521 tccagaacgt ggcgggtttg cgcctttggg tcatcggcaa acagcacgt
//
LOCUS       DAEEKQ010000217         5436 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.219, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000217 DAEEKQ010000000
VERSION     DAEEKQ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.219"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            508..2793
                     /gene="nrdA"
                     /locus_tag="KLK57_003850"
     CDS             508..2793
                     /gene="nrdA"
                     /locus_tag="KLK57_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HBE0894100.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINNLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDTIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3027..4157
                     /gene="nrdB"
                     /locus_tag="KLK57_003851"
     CDS             3027..4157
                     /gene="nrdB"
                     /locus_tag="KLK57_003851"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HBE0894101.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4157..4411
                     /gene="yfaE"
                     /locus_tag="KLK57_003852"
     CDS             4157..4411
                     /gene="yfaE"
                     /locus_tag="KLK57_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HBE0894102.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(4488..5114)
                     /gene="inaA"
                     /locus_tag="KLK57_003853"
     CDS             complement(4488..5114)
                     /gene="inaA"
                     /locus_tag="KLK57_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005136699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HBE0894103.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRNKAINHDFRQLENIWSQSPKRTGSR"
     gene            5293..5436
                     /locus_tag="KLK57_003854"
                     /pseudo
     CDS             5293..5436
                     /locus_tag="KLK57_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         1369 a         1441 c         1312 g         1314 t
ORIGIN      
        1 tagtaatgaa taattttctt ataatataag gtaaattaac aaaatggctt cgcatttaac
       61 aataaccgaa tagaaaacaa ccatttcgac atcaacaatc gcttacattc gcttatatat
      121 tgaccacaac tgatacatca gattatgtga tgactcgtgc ttagatcaat ttttgcaatc
      181 attaacaaaa agattaataa accatctata tcaatttatc caacctattc tgccgttcaa
      241 gaaatcgccg aacagttatt tttaacaaat ttttctcttc ccattgactt tcccggacac
      301 cttgtctgac ctaaggtgcg cgaaagccac tttttccttc ctgagttatc cacaaagtta
      361 tgcacttgca agagggtcat tttcacacta tcttgcagtg aatcccaaac atacccccta
      421 tatatagtgt tctaagcagc ttcccttact acaggtagtc tgcatgaaac tattgcggaa
      481 agaattccaa aaacaggtac gacatacatg aatcagaatc tgctggtgac aaagcgcgac
      541 ggtagcacag agcgcatcaa tctcgacaaa atccatcgcg ttctggattg ggcggcagaa
      601 ggactgcata acgtttcgat ttcccaggtc gagctgcgct cccacattca gttttatgac
      661 ggtatcaaga cctctgacat ccacgaaacc attatcaagg ctgccgcaga cctgatctcc
      721 cgtgatgcgc cggattatca gtatctcgcc gcgcgcctgg cgatcttcca cctgcgtaaa
      781 aaagcctacg gccagtttga gccgcctgcg ctgtacgacc acgtggtgaa aatggtcgag
      841 atgggcaaat acgataatca tctgctggaa gactacacgg aagaagagtt caagcagatg
      901 gacacgttta tcgatcacga ccgtgatatg accttctctt atgctgccgt taagcagctg
      961 gaaggcaaat atctggtaca gaaccgcgtg accggcgaaa tctatgagag cgcccagttc
     1021 ctttatattc tggttgccgc gtgcctgttc tcgaactacc cgcgtgaaac gcgcctgcaa
     1081 tatgtgaagc gtttttacga cgcggtttcc acatttaaaa tttcgctgcc gacgccaatc
     1141 atgtccggcg tgcgtacccc gactcgtcag ttcagctcct gcgtactgat cgagtgcggt
     1201 gacagcctgg attccatcaa cgccacctcc agcgcgattg ttaaatacgt ttcccagcgt
     1261 gccgggatcg gcatcaacgc cgggcgtatt cgtgcgctgg gtagcccgat tcgcggtggt
     1321 gaagcgttcc ataccggctg cattccgttc tacaaacatt tccagacagc ggtgaaatcc
     1381 tgctctcagg gcggtgtgcg cggcggtgcg gcaacgctgt tctacccgat gtggcatctg
     1441 gaagtggaaa gcctgctggt gttgaaaaac aaccgtggtg tggaaggcaa ccgcgtgcgt
     1501 catatggact acggggtaca aatcaacaaa ctgatgtata cccgtctgct gaaaggtgaa
     1561 gatatcaccc tgttcagccc gtccgacgta ccggggctgt acgacgcgtt cttcgccgat
     1621 caggaagagt ttgaacgtct gtataccaaa tatgagaaag acgacagcat ccgcaagcag
     1681 cgtgtgaaag ccgttgagct gttctcgctg atgatgcagg aacgtgcgtc taccggtcgt
     1741 atctatattc agaacgttga ccactgcaat acccatagcc cgtttgatcc ggccatcgcg
     1801 ccagtgcgtc agtctaacct gtgcctggag atagccctgc cgaccaaacc gctgaacgac
     1861 gtcaacgacg agaacggtga aatcgcgctg tgtacgctgt ctgctttcaa cctgggcgca
     1921 attaataacc tggatgaact ggaagagctg gcaattctgg cggttcgtgc acttgacgcg
     1981 ctgctggatt atcaggatta cccgatcccg gccgccaaac gtggagcgat gggtcgtcgt
     2041 acgctgggta ttggtgtgat caacttcgct tactacctgg cgaagcacgg taaacgctac
     2101 tccgacggca gcgccaacaa cctgacgcat aaaaccttcg aagccattca gtattacctg
     2161 ctgaaagcct ctaatgagct ggcgaaagag caaggcgcgt gcccgtggtt taacgaaacc
     2221 acttacgcga aagggatcct gccgatcgat acctataaga aagatctgga taccatcgct
     2281 aatgagccgc tgcattacga ctgggaagca ttgcgtgaat caatcaaaac gcacggcctg
     2341 cgtaactcta cgctttctgc cctgatgccg tccgagacct cttcgcagat ctctaacgcc
     2401 actaacggta ttgaaccgcc gcgcggttac gtcagcatca aagcgtcgaa agacggtatt
     2461 ttgcgccagg tggtgccgga ctacgagcac ctgcacgacg cctatgagct gctgtgggaa
     2521 atgccgggta acgatggcta cctgcaactg gtaggtatca tgcagaaatt tatcgatcag
     2581 tcgatctctg ctaacaccaa ctacgatccg tcacgtttcc cgtcaggaaa agtgccgatg
     2641 cagcagttgc tgaaagacct gctcaccgcc tacaaattcg gggtcaaaac gctgtattat
     2701 cagaacaccc gtgacggcgc tgaagacgcg caggacgatc tggtaccgtc aatccaggac
     2761 gatggctgcg aaagcggcgc atgtaagatc tgatattgag atgccggatg cggcgtaaac
     2821 gccttatccg gcctacggct cggtttgtag gcctgataag acgcgccagc gtcgcatcag
     2881 gctccgggtg ccggatgcag cgtgaacgcc ttatccggcc tacggctcgg atttgtaggc
     2941 ctgataagac gcgccagcgt cgcatcaggc acaggatgcg gcgtaaaatg ccttatccgg
     3001 cattaaactc ccaacaggac acactcatgg catataccac cttttcacag acgaaaaatg
     3061 atcagctcaa agaaccgatg ttctttggtc agccggtcaa cgtggctcgc tacgatcagc
     3121 aaaaatatga catcttcgaa aagctgatcg aaaagcagct ctctttcttc tggcgtccgg
     3181 aagaagttga cgtctcccgc gaccgtatag attaccaggc gctgccggag cacgaaaaac
     3241 acatctttat cagcaacctg aaatatcaga cgctgctgga ttccattcag ggtcgtagcc
     3301 cgaacgtggc gctattgccg cttatttcta ttccggaact ggaaacctgg gtcgaaacct
     3361 gggcgttctc agaaacgatt cattcccgtt cctatactca tatcattcgt aatatcgtta
     3421 acgatccgtc tgttgtgttt gacgatatcg tcaccaacga gcagatccag aaacgtgcgg
     3481 aagggatctc cagctattac gatgaactga tcgaaatgac cagctactgg caccttctgg
     3541 gcgaaggcac ccacaccgtt aacggtaaaa ccgtaaccgt tagcctgcgc gagctgaaga
     3601 aaaaactgta tctctgcctg atgagcgtta acgcgctgga agcgatccgc ttctacgtca
     3661 gctttgcttg ttcctttgcc tttgcagaac gcgaactgat ggaaggtaac gccaagatta
     3721 ttcgcctgat tgcccgcgat gaagccctgc acctgaccgg tactcagcat atgctgaatc
     3781 tgctgcgcag cggcgcggat gatccggaga tggcggaaat tgccgaagaa tgtaagcagg
     3841 agtgctatga cctgtttgtc caggcggcac agcaagagaa agactgggcg gattatctgt
     3901 tccgtgacgg ttcgatgatt ggtctgaata aagatattct ctgccagtac gttgaataca
     3961 tcaccaatat ccgcatgcag gcagtcggtt tggatctgcc gttccagacg cgctccaacc
     4021 cgatcccgtg gatcaacacc tggctggtgt ctgataacgt gcaggttgct ccgcaggaag
     4081 tggaagtcag ttcttatctg gtcgggcaga ttgacgcgga agtggacacc gacgatttga
     4141 gtaacttcca gctctgatgg cccgcgttac cctgcgcatc actggcacac aactgctgtg
     4201 ccaggatgaa cacccttccc ttctggcggc gctggaatcc cacaatgttg cggttgagta
     4261 ccagtgccgc gaaggttact gcggctcctg tcgcacccga ctggtcgcag gtcaggtgga
     4321 ctggattgcc gaaccgttag cctttattca gccgggggaa attttgccct gttgttgccg
     4381 ggcaaaaggc gatattgaaa tcgagatgtg aattggttgt agtgccagat acaacgctta
     4441 tacgtcttat ctggcctaca acgattacat ggcgtagtaa tacgctttca cctgctccca
     4501 gtccgctttg gggattggct ccagatattt tccagctggc ggaagtcatg attaatcgct
     4561 ttattgcgac gcaagcggcg acgacttttc tccaggtcaa gaaaaccagc ttctgcttta
     4621 ccttctgttt tcacatagat atggcgaaca taacaacagc catgctgacg attaacgcta
     4681 tgcattttct taaacgccag cgccactgct ttcaacatgg cttgccgtac ttcgtcagaa
     4741 tagggcgata ctgcatgccg ggcataccag tcagcaatgc tgatgaaccc cgccatatct
     4801 tcagtcacca gcaacgcgcg ccattcaccc tcaattttca ccgcttcacc aaaaacgatc
     4861 ttcggcacga tgacaccggc ccgttccagt tctttaatta ccgcaacctc acggacaatc
     4921 gttggtcggc cgaacggata acgtacggaa tgaaacagat gatgcgtcat gcgctttaca
     4981 tacagctttt tgccgttgcg ctcgacgcat tgcaccccgc tcataccatt acggcgatag
     5041 ttaggctctt caacccagtc gccctctgtt gcccaccagt gattaaattc gtcgtacttt
     5101 gctgaaactg ccatacccta tcgcctgtca tttttattaa cgacaatgac tataggtggt
     5161 tacctgagga aaatcttaat gaaacgtgtc gtattaatga atttcttttg ccgctactag
     5221 tgtgacaccg cttttgccct tcatttgcag cgcatagatt ccaccagcca cgaccactaa
     5281 tagtgcgccc agcagataaa atgtgaattt aatcaggtgg atacagcgat gaattttatt
     5341 cgacagggat taggcatcgc cttattacca gagttaacgc tgaaaaccat tgcaggtgaa
     5401 ttgtgttccg ttcctcacga accaaccttc tatcga
//
LOCUS       DAEEKQ010000218         5391 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.220, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000218 DAEEKQ010000000
VERSION     DAEEKQ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5391
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.220"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            236..1093
                     /gene="focA"
                     /locus_tag="KLK57_003855"
     CDS             236..1093
                     /gene="focA"
                     /locus_tag="KLK57_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HBE0894104.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            1148..3430
                     /gene="pflB"
                     /locus_tag="KLK57_003856"
     CDS             1148..3430
                     /gene="pflB"
                     /locus_tag="KLK57_003856"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HBE0894105.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDVLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            3622..4362
                     /gene="pflA"
                     /locus_tag="KLK57_003857"
     CDS             3622..4362
                     /gene="pflA"
                     /locus_tag="KLK57_003857"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HBE0894106.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(4455..4631)
                     /locus_tag="KLK57_003858"
     CDS             complement(4455..4631)
                     /locus_tag="KLK57_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005043118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HBE0894107.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNIISRYYLRYGIW"
     gene            4731..>5391
                     /locus_tag="KLK57_003859"
     CDS             4731..>5391
                     /locus_tag="KLK57_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0894108.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKEQEIAVAGNI
                     IL"
BASE COUNT         1387 a         1321 c         1290 g         1393 t
ORIGIN      
        1 cgcctgatgc tcccttttaa ttaactgttt tagcggagga tgcggaaaaa attcaactca
       61 tttgttaatt tttaaaattt atttttattt ggataatcaa atgtttactc cgtatctgca
      121 taaaaaccat gcgagttacg ggcctataag ccaggcgaga tatgatctat atcaatttct
      181 catctataat gctttgttag tatctcgtcg ccgacttaat aaagagagag ttagtgtgaa
      241 agctgacaac ccttttgatc ttttacttcc tgctgcaatg gccaaagtgg ccgaagaggc
      301 gggtgtctat aaagcaacga aacatccgct taagactttc tatctggcga ttaccgccgg
      361 tgttttcatc tcaatcgcat tcgtcttcta tatcacagca accactggca caggcacaat
      421 gcccttcggc atggcaaaac tggttggcgg catttgcttc tctctggggc tgattctttg
      481 tgttgtctgc ggagccgatc tctttacttc caccgtgttg attgttgttg ctaaggcgag
      541 tgggcgcatc acctggggtc agttggcgaa aaactggcta aatgtctatt ttggcaacct
      601 ggtcggcgca ctgctgtttg tacttttaat gtggctttcc ggcgagtata tgaccgcaaa
      661 tggtcaatgg ggactaaacg tcctacaaac cgccgaccac aaagtgcacc atacttttat
      721 tgaggccgtc tgtcttggta tcctggcaaa cctgatggta tgtctggcag tatggatgag
      781 ttattctggc cgcagcctga tggacaaagc gttcattatg gtgctgccgg tcgcgatgtt
      841 tgttgccagc ggttttgagc acagtatcgc aaacatgttt atgatcccga tgggtattgt
      901 aatccgcgac ttcgcatccc cggaattttg gaccgcagtc ggttctgcac cggaaaattt
      961 ttctcacctg accgtgatga atttcatcac tgataacctg attccggtta cgatcggcaa
     1021 cattatcggt ggtggtttgt tggttgggtt gacatactgg gtcatttacc tgcgtgaaaa
     1081 cgaccaccat taatggttgt cgaagtacgc agtaaataaa aaatccactt aagaaggtag
     1141 gtgttacatg tccgagctta atgaaaagtt agccacagcc tgggaaggtt ttaccaaagg
     1201 tgactggcag aatgaagtaa acgtccgtga cttcattcag aaaaactaca ctccgtacga
     1261 gggtgacgag tccttcctgg ctggcgctac tgaagcgacc accaccctgt gggacaaagt
     1321 aatggaaggc gttaaactgg aaaaccgcac tcacgcgcca gttgactttg acaccgctgt
     1381 tgcttccacc atcacctctc acgacgctgg ctacatcaac aagcagcttg agaaaatcgt
     1441 tggtctgcag actgaagctc cgctgaaacg tgctcttatc ccgttcggtg gtatcaaaat
     1501 gatcgaaggt tcctgcaaag cgtacaaccg cgaactggat ccgatgatca aaaaaatctt
     1561 cactgaatac cgtaaaactc acaaccaggg cgtgttcgac gtttacactc cggacatcct
     1621 gcgttgccgt aaatctggtg ttctgaccgg tctgccagat gcatatggcc gtggccgtat
     1681 catcggtgac taccgtcgcg ttgcgctgta cggtatcgac tacctgatga aagacaaact
     1741 ggcacagttc acttctctgc aggctgatct ggaaaacggc gtaaacttgg aacagactat
     1801 ccgtctgcgc gaagaaatcg ctgaacagca ccgcgctctg ggtcagatga aagaaatggc
     1861 tgcgaaatac ggctacgaca tctctggtcc ggctaccaac gctcaggaag ctatccagtg
     1921 gacttacttc ggctacctgg ctgctgttaa gtctcagaac ggtgctgcaa tgtccttcgg
     1981 tcgtacctcc accttcctgg atgtgtacat cgaacgtgac ctgaaagctg gcaagatcac
     2041 cgaacaagaa gcgcaggaaa tggttgacca cctggtcatg aaactgcgta tggttcgctt
     2101 cctgcgtact ccggaatacg atgaactgtt ctctggcgac ccgatctggg caaccgaatc
     2161 tatcggtggt atgggcctcg acggtcgtac cctggttacc aaaaacagct tccgtttcct
     2221 gaacaccctg tacaccatgg gtccgtctcc ggaaccgaac atgaccattc tgtggtctga
     2281 aaaactgccg ctgaacttca agaaattcgc cgctaaagtg tccatcgaca cctcttctct
     2341 gcagtatgag aacgatgacc tgatgcgtcc ggacttcaac aacgatgact acgctattgc
     2401 ttgctgcgta agcccgatga tcgttggtaa acaaatgcag ttcttcggtg cgcgtgcaaa
     2461 cctggcgaaa accatgctgt acgcaatcaa cggcggcgtt gacgaaaaac tgaaaatgca
     2521 ggttggtccg aagtctgaac cgatcaaagg cgatgtcctg aactatgatg aagtgatgga
     2581 gcgcatggat cacttcatgg actggctggc taaacagtac atcactgcac taaacatcat
     2641 ccactacatg cacgacaagt acagctacga agcctctctg atggcgctgc acgaccgtga
     2701 cgttatccgc accatggcgt gtggtatcgc tggtctgtcc gttgctgctg actccctgtc
     2761 tgcaatcaaa tatgcgaaag ttaaaccgat tcgtgacgaa gacggtctgg ctatcgactt
     2821 cgaaatcgaa ggcgaatacc cgcagtttgg taacaacgat ccgcgtgtag atgacctggc
     2881 tgttgacctg gtagaacgtt tcatgaagaa aattcagaaa ctgcacacct accgtgacgc
     2941 tatcccgact cagtctgttc tgaccatcac ttctaacgtt gtgtatggta agaaaacggg
     3001 taacacccca gacggtcgtc gtgctggcgc gccgttcgga ccgggtgcta acccgatgca
     3061 cggtcgtgac cagaaaggtg cagtagcctc tctgacttcc gttgctaaac tgccgtttgc
     3121 ttacgctaaa gatggtatct cctacacctt ctctatcgtt ccgaacgcac tgggtaaaga
     3181 cgacgaagtt cgtaagacca acctggctgg tctgatggat ggttacttcc accacgaagc
     3241 atccatcgaa ggtggtcagc acctgaacgt taacgtgatg aaccgtgaaa tgctgctcga
     3301 cgcgatggaa aacccggaaa aatatccgca gctgaccatc cgtgtatctg gctacgcagt
     3361 acgtttcaac tcgctgacta aagaacagca gcaggacgtt attactcgta ccttcactca
     3421 atctatgtaa ttagatttga ctgaaatcgt acagtaaaaa gcgtacaata aaggctccac
     3481 gaaagtgggg ccttttttag cacgagagcc ttttttgtca gctatctata ctctaaggtg
     3541 actgccaaaa cagactcgac gtagccttcg agctgcgcac caacacggcc tcagatgggc
     3601 cacatctgga gaaacaccgc aatgtcagtt attggtcgca ttcactcctt tgaatcctgt
     3661 ggaaccgtag acggcccggg tattcgcttt atcacctttt tccagggctg cctgatgcgc
     3721 tgcctgtatt gtcataaccg cgacacctgg gatacgcatg gcggtaaaga agttaccgtt
     3781 gaagatttaa tgaaagaggt ggtgacctat cgccacttta tgaacgcttc cggcggcggc
     3841 gttaccgcat ccggcggtga ggcaatccta caagctgagt ttgttcgtga ctggttccgc
     3901 gcctgcaaaa aagaaggcat tcatacctgt ctggacacca acggttttgt tcgtcgttac
     3961 gatccggtga ttgatgaact gctggaagta accgacctgg taatgctcga tctcaaacag
     4021 atgaacgacg agatccacca aaatctggtt ggagtttcca atcaccgcac gctggagttc
     4081 gccaaatatc tggcgaacaa aaatgtgaag gtgtggatcc gctacgttgt tgtcccaggc
     4141 tggtctgacg atgacgattc agcgcatcgc ctcggtgaat ttacccgtga tatgggcaac
     4201 gttgagaaaa tcgagcttct cccctaccac gagctgggca aacacaaatg ggtggcaatg
     4261 ggtgaagagt acaaactcga cggtgttaaa ccaccgaaga aagagaccat ggaacgcgtg
     4321 aaaggcattc ttgagcagta cggtcataag gtaatgttct aatttccccg taaagcggca
     4381 actttattga gttgccgctt ttttattccg cacttcattc tgctacagcg tttttaacac
     4441 atcttatagt gcatctacca tatcccgtac ctgagataat aacgactgat aatattacgc
     4501 cgatataaat cgagttccgt cacctgtatt ttccgctcca ttgcccgctg atggatcgca
     4561 tcagcaatat gtgcggtcaa tgaatcatga cgaggatggg cccataccaa ataaatacgt
     4621 tcagactgca taattttacc tccacgttaa gttctggagc gcactttacg caggcggcat
     4681 aataatgtaa acgtgatgat tcaggatgag ttaatgaagg attttcacta atgcggatga
     4741 atatgtctga ctttgccact ttctttgctg tggcccgtaa tcaaagcttt cgtgcagcgg
     4801 gcgatgagtt aggcttatcc tcgtccgcca ttagccatag tattaaaaca ctggaacaac
     4861 gtcttaaaat tcgcttattc aatcggacaa cccgaagcgt atcgttaacg gaagcgggtt
     4921 ctaatctcta tgaacgattg cgcccagctt tcgacgaaat tcagatcatg ttagatgaga
     4981 tgaatgattt tcgtctgaca ccaacaggaa cgctcaaaat caatgccgcc cgggtagctg
     5041 cacgtatatt tttaatgcct ttactggttg gtttcacacg tgaatatcct gatattaaag
     5101 tcgagctcac aactgacgac tctcttgtcg atatcgtcca acagggcttt gacgctggcg
     5161 ttcgacttag tggcatcgtc gaaaaagata tgatcagcgt ggcgatcggc ccaccagtga
     5221 aattatgtgt agcagcaacg ccagagtact ttgcaagata tggcaaaccc cgccatcctc
     5281 atgatctgct taatcatcaa tgtgttgtat ttcgttatcc cagcggcaaa cctttccact
     5341 ggcaatttgc aaaggagcag gaaattgctg tcgcagggaa tatcattctg g
//
LOCUS       DAEEKQ010000219         5339 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.221, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000219 DAEEKQ010000000
VERSION     DAEEKQ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5339
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.221"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(55..1338)
                     /gene="aroA"
                     /locus_tag="KLK57_003860"
     CDS             complement(55..1338)
                     /gene="aroA"
                     /locus_tag="KLK57_003860"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HBE0894109.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1409..2497)
                     /gene="serC"
                     /locus_tag="KLK57_003861"
     CDS             complement(1409..2497)
                     /gene="serC"
                     /locus_tag="KLK57_003861"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HBE0894110.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2696..3388)
                     /locus_tag="KLK57_003862"
     CDS             complement(2696..3388)
                     /locus_tag="KLK57_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HBE0894111.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3518..5278
                     /locus_tag="KLK57_003863"
     CDS             3518..5278
                     /locus_tag="KLK57_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HBE0894112.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1365 a         1379 c         1266 g         1329 t
ORIGIN      
        1 atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt tcattcaggc
       61 tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg ccgtacattt
      121 gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac acatcgccat
      181 ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggtg gagtgatacg
      241 aatgtaatcg tgcccctctt ctacttccgc accgacttta cgcagttctg ttgccatcgc
      301 aaacaggcga tcggtctctt taacacgcca gttatagata ttgcgcagcg tggtggtgcc
      361 ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcgggaatat ggttcatatc
      421 catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc cccagcaaat
      481 ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca tactgttacg
      541 tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca gaaagtaaga
      601 agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag actgcccgcc
      661 ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg tcttcatcag
      721 attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac gaatcaccgt
      781 atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc tggaaacgga
      841 gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg gcggataatt
      901 ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca ccagatgacc
      961 aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc tacccagaca
     1021 aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca actccagggc
     1081 accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac gatcggctga
     1141 aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca cgtcatcgct
     1201 atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca ataaagcgcg
     1261 gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag cgatgggttg
     1321 taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc ccacaggctg
     1381 gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac tcaaccatga
     1441 agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa gcgcgcattc
     1501 cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac tcttcaagga
     1561 acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc gaacggttag
     1621 ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat agcagttctg
     1681 ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc agccatttaa
     1741 agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg gagccgttat
     1801 cgttgaggat ggaataatcc agaatcgacg gacacgcgat attcgctttg cccagcaaat
     1861 cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc gcgccagcgt
     1921 aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa gagaagtcgg
     1981 cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc gatggcgata ccgtcgatgg
     2041 tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat tcacgcattg
     2101 gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta ggcgtgcagt
     2161 attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca taatctgcgg
     2221 tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga ccaccgccat
     2281 ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa tccttctcgg
     2341 cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc accgacgtac
     2401 caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct gccggtagca
     2461 ttgccggacc agaactaaaa ttgaagattt gagccattgc ccctcaccac gttgcgttgc
     2521 tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg tggctatcgg
     2581 ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa aaatgtggct
     2641 tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt tactgtcagg
     2701 tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc ggacataatt
     2761 gtttttcccc cgctttcata tgaatgattt cactacaacg aatacaggca tagtccgccg
     2821 actccggcac cactttgtaa cgctgcgtac aatcactggg taaaataagt aaacccggtt
     2881 tcaccttgtc atcttcaaag aaatagacac tgatttgccc gttggtttcg agaatcgcca
     2941 gacgtacctg ccccagctgc tccacgccac gcaatcgtag ctccataaag aactcaaatt
     3001 ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca ataataacaa
     3061 ccggcttacc ttccagtaga tcttccagtt tttcactgtg cgccatcaac cacattacca
     3121 ggcggtataa caacgccaga gtgataaaga cgataagtac cggcaccatc ggcacatcat
     3181 cataaaacgc cacatctccc gccgctgaac ccagcgtgag aatgattaac acttcaaaca
     3241 gcgacatctg tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa accagtacaa
     3301 aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact ttatcaaatg
     3361 ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt atccgctaaa
     3421 agcatagtca acagttcatc ggcatggcgg cggtgatagc gcctttgaga taaaacccgt
     3481 attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat ttatccccgg
     3541 caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt cagacctcgg
     3601 ctttcagatt gaagaggcct cctggctgaa tcctgtgcct aacgtctggt ctgtacatat
     3661 tcgcgacaaa gagtgcgcac tgtgttttac caacggtaaa ggcgcaacca aaaaagcggc
     3721 gctggcttct gcacttggtg aatatttcga gcgtctctca accaactact tttttgctga
     3781 tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg cattatccca acgaaaaatg
     3841 gttcccactg accgaaaatg acgatgtgcc agaaggacta ctcgatgacc gtctgcgcgc
     3901 gttttatgat ccggagaatg aactgaccgg cagcatgctg attgacctac aatccggtaa
     3961 cgaagatcgt ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc agaccgttta
     4021 tattccgatg aatatcattg gtaacctgta cgtctccaac ggtatgtccg caggtaatac
     4081 ccgcaacgaa gcacgcgttc agggattgtc tgaagttttc gaacgctacg tgaaaaaccg
     4141 cattattgct gaaagcatca gcctgccaga gatcccggca gacgtgctgg cgcgttaccc
     4201 agcagtagtt gaagccatcg aaacgctgga agcggagggt ttcccaatct tcgcttatga
     4261 cggttcgctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc ctgctaacgg
     4321 tacctgcttt gcctctttcg gtgcgcatcc tgattttggc gtagcactgg aacgtaccgt
     4381 gaccgagctg ctgcaaggtc gtggcctgaa agatttggat gtgtttactc cgccaacctt
     4441 cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg attccagcgg
     4501 tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgtgg actggaattt
     4561 ctccggcacc acggaagaag agtttgctac gctgatggct atcttcaaca aagaagataa
     4621 agaagtttat attgccgatt acgagcatct gggcgtttat gcttgccgta ttatcgtgcc
     4681 tggcatgtcc gatatttatc cggctgaaga tctgtggctc gcgaataaca gtatgggcag
     4741 ccatttacgt gaaacgattc tttcgctacc aggcagcgag tgggaaaaag aagattacct
     4801 gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg tgcgtgagct
     4861 gttgggtctg gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg gtgaattaaa
     4921 agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga ccgaatggac
     4981 gatggagttt aactcatcag tatttagtcc ggaacgcgcc aactattatc gctgcctgca
     5041 aacgttgtta ttactggcac aggaagaaga tcgccagccg ctgcaatatc tgaatgcgtt
     5101 tgttcgcatg tacggcgcag atgccgtaga agccgccagt gcggcaatga gcggcgaagc
     5161 ggcgttttac ggcctgcaac cggtagatag cgatctgcac gcgtttgctg cacatcagtc
     5221 gttgttgaag gcctacgaaa agctgcagcg cgccaaagca gcattctggg caaaataaaa
     5281 tcaaatagcc tacgcaatgt aggcttaatg attagtctga gttatattac ggggcgttt
//
LOCUS       DAEEKQ010000220         5322 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.222, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000220 DAEEKQ010000000
VERSION     DAEEKQ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5322
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.222"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            283..630
                     /locus_tag="KLK57_003864"
     CDS             283..630
                     /locus_tag="KLK57_003864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HBE0894113.1"
                     /translation="MILDSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFI
                     GSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEI
                     GAVPAIPQQSSLF"
     gene            683..1078
                     /locus_tag="KLK57_003865"
     CDS             683..1078
                     /locus_tag="KLK57_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HBE0894114.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1119..1862
                     /gene="cmoA"
                     /locus_tag="KLK57_003866"
     CDS             1119..1862
                     /gene="cmoA"
                     /locus_tag="KLK57_003866"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HBE0894115.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCSRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            1859..2830
                     /gene="cmoB"
                     /locus_tag="KLK57_003867"
     CDS             1859..2830
                     /gene="cmoB"
                     /locus_tag="KLK57_003867"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HBE0894116.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(2995..>5322)
                     /gene="torZ"
                     /locus_tag="KLK57_003868"
     CDS             complement(2995..>5322)
                     /gene="torZ"
                     /locus_tag="KLK57_003868"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HBE0894117.1"
                     /translation="DGKILTAERWGAMNVEVKDGKIVSSTGALAKTIPNSLQSTAADQ
                     VHTTTRIQHPMVRKSYLDNPLQPTKGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPS
                     AIFAGSYGWRSSGVLHKAQTLLQRYMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVY
                     EQQTSWPLILENSQVVVLWGMNPLNTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPI
                     RSETIDFFGDNATWIAPNMGTDVALMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYL
                     RGKSDNTPKSAAWAAEITGVPEAQIVKLAELMAANRTMLMAGWGIQRQQYGEQKHWML
                     VTLAAMLGQIGTPGGGFGFSYHYSNGGNPTRVGGVLPEMSAAIAGQASEAADDGGMRA
                     IPVARIVDALENPGGKYQHNGKEQTYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEM
                     IVVSECYWTAAAKHADIVLPITTSFERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDF
                     DVFADLAELLKPGGKEIYTEGKDEMAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKL
                     IEMRRSEKNEQYVRYGDFRADPVKNALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAP
                     DEWKGTADEKQLQLLTAHPAHRLHSQLNYAELRKKYAVADREPITIHTEDAARFSIAN
                     GDLVRVWNKRGQILTGAVVTDGIKKGVVCVHEGAWPDLENGLCKNGSANVLTADIPSS
                     QLANACAGNSALVYIEKYTGNAPKLTAFDKPAVQA"
BASE COUNT         1212 a         1376 c         1289 g         1445 t
ORIGIN      
        1 acgactaaca cgatacagtt taccctgaaa agagacttca cagattcttt atatatttca
       61 ataagttatg ctaaaaagag actataccga tactgcatag aatttgtgta ggattgcata
      121 gatacttgga aaaacaccgt taaattgcat agtcattcac ctgatattgt cctgttttta
      181 caggtagttg attcggatgt tctaggaatc atcctcatag ttgattcggt tagttgggct
      241 tgtaccaacc cctaatcaac ctcatagtgt atttagacct tgatgatttt agacagccgc
      301 cctgttcatg cagcacgtcc acacagtgaa gctattcgcg acgctcaacg aaaaaaacct
      361 aaagttccgg tacatgctgt actgacggcg acaaatccgc tgattcgttt tatcggtagt
      421 gatgatatga cgcaaaaccg ggaattattt caggtctggt tacagaaatt agcgcagtgg
      481 catcagacca ctacgcctta tcttttttta catacgccag acattgccca ggccccggaa
      541 ctggtacata ccctgtggga agacttacgt aaaacgcttc cagagatcgg agcagttccg
      601 gctattccac agcaatcttc tcttttctga atttgccacc tatcatagac aggtgccatc
      661 ggccatttta aagggagttt gtatggtaag cgcgctatat gccgttttaa gtgcgttgtt
      721 attaatgaag ttctcttttg atgtcgttcg cctgcgaatg cagtaccgcg ttgcctatgg
      781 tgacggtggt tttagcgaac tgcaaagcgc tattcgcatt catggtaacg cggtggaata
      841 tattcccatc gcgattgtgc tgatgctgtt tatggaaatg aatggcgcag aaacctggat
      901 ggtgcatatt tgcggcatcg ttttgcttgc tggtcgtctg atgcattatt acggttttca
      961 tcaccgtctg tttcgctggc gacgttccgg catgagcgcc acctggtgtg cgctgttgct
     1021 gatggtgctg gcgaatcttt ggtatatgcc ctgggagttg gttttctccc tgcgttagcg
     1081 cacaatacgc cactttcttt ttcccggatt tttacgttat gtctcaccgc gacacgctat
     1141 tttctgcccc tatcgccaga ctgggcgact ggacctttga tgaacgggta gctgaagtct
     1201 tcccggatat gatccagcgt tccgttcccg gctattccaa tattatttcc atgattggta
     1261 tgttagccga gcgcttcgtt caacctggta cgcaggttta cgatctgggt tgttctctgg
     1321 gcgcggcgac gctctcggtg cgtcgcaaca ttcatcatga taattgcaaa attattgcca
     1381 tcgacaactc cccggcgatg attgaacgct gcagtcgtca tattgacgcc tataaagccc
     1441 ctacgccagt agacgtcatt gaaggtgata ttcgcgatat cgccattgaa aacgcatcga
     1501 tggtggtgct gaattttacc ctgcaattcc tggaaccttc cgagcgccag gcattactgg
     1561 ataaaattta tcaaggactg aacccgggcg gcgcgctggt gctttcggaa aaattcagtt
     1621 tcgaagatgc caaagttggt gaactgttgt tcaacatgca ccacgacttt aaacgtgcca
     1681 acggttacag cgaactggag atcagccaga agcgcagcat gctggaaaac gtgatgctga
     1741 ccgattccgt ggaaacccat aaagcacgcc tgcataaagc cggttttgag catagcgagc
     1801 tgtggttcca gtgctttaac tttggttcac tggtggcatt aaaagcagag gacgctgcat
     1861 gatcgacttt ggtaactttt attctctgat tgccaaaaat catctttcac actggctcga
     1921 aacgctgccc gcgcagattg ctaactggca gcgcgagcag cagcacgggc tgtttaagca
     1981 gtggtccaac gcggtggaat ttctgcctga aattaaaccg tatcgtctgg atttattgca
     2041 tagcgtaacc gccgaaagcg aagagccact gagcaccggg caaattaaac gcattgaaac
     2101 gcttatgcgg aacctgatgc catggcgcaa agggccgttc tcactgtatg gcgtcaacat
     2161 cgataccgaa tggcgttccg actggaaatg ggatcgtgtt ctgccccatc tttctgattt
     2221 aaccggacgc accattctgg atgtcggctg cggcagcggt tatcacatgt ggcgcatgat
     2281 tggcgcaggc gcgcacctcg cggtggggat cgaccctacg caactgtttc tgtgccagtt
     2341 tgaagcggtg cgtaaactac tgggtaacga tcagcgcgca catttgttac cgttaggtat
     2401 tgaacagctt ccggcactga aagcctttga taccgtcttt tcgatgggcg tgctttacca
     2461 tcgccgttcg ccgctggagc atctctggca gttgaaagat caactggtga atgagggtga
     2521 actggtgctg gaaacgctgg ttattgatgg cgatgagaac acggtgctgg taccaggcga
     2581 tcgttacgcg caaatgcgta atgtctattt catcccttcc gcgctggcgc tgaaaaactg
     2641 gctgaagaag tgtggttttg tagatatccg cattgtggat gtgtgcgtta ccactactga
     2701 agagcagcga cgcactgaat ggatggttac cgaatctctg tccgatttcc tcgacccaca
     2761 tgatccgagt aagacagttg aaggttatcc tgcgcctaaa cgcgcggtgc tgattgcgcg
     2821 caagccgtaa aggtctggta ttactgccgg atgcggcgtg aatgccttat ccggcctaca
     2881 aacccttgct aattcaatct attgcaggga ctatgtaggc ctgataagca tagcgcatca
     2941 ggcagtttta cgtttgcata acctcagcgc ccgtttccgg gcgctattca cttcttacgc
     3001 ctgaacagca ggcttatcaa atgccgttaa cttcggtgcg ttacccgtat atttttcgat
     3061 atacaccagc gcagagttac cggcacaggc atttgccagc tgcgagctgg ggatatccgc
     3121 cgttaacacg ttcgcactgc cgtttttaca caagccgttt tccagatctg gccatgcacc
     3181 ttcatgcacg cataccacgc cttttttgat cccgtcagtc accaccgcgc ctgtcaaaat
     3241 ctgaccacgt ttgttccaga cacgcaccag atcgccattc gcaatactaa atcgagcagc
     3301 atcttcggtg tgaatagtga tcggttcacg atcggcaacc gcatattttt tacgcagttc
     3361 cgcatagtta agctgactgt gtaaacggtg tgccggatgt gcggtcagaa gctgcaactg
     3421 cttctcgtcg gcggtaccct tccactcatc aggcgcaagc caggttgggt gtgccgggca
     3481 atccttatag ccaaattttt ccagcgtttt ggagtaaatc tcaattttgc cacttggcgt
     3541 acccagcgca tttttcaccg gatcggcgcg gaaatcacca taacgaacgt actgttcgtt
     3601 cttctcgctg cggcgcattt cgatcagttt attttgctgc cagaaggcat taaacattgg
     3661 catagtgacg cgttgcgcac gggccccttt ctgagcggca tcatagaaaa atttcagcca
     3721 cgccatttca tctttacctt cggtatagat ctcttttccg ccaggtttga gtaattccgc
     3781 aagatcggca aacacatcaa aatcgtttcg cgcttcaaat tgcggagcaa cagcctgctt
     3841 catcggcaca atatgctggt tgctgtaatc accggtcatc gtcaagtcat tgcgctcaaa
     3901 cgatgtggtg atcggtaata cgatatctgc gtgtttagca gctgcggtcc agtagcattc
     3961 agaaacgacg atcatctccg gtttctgcca tgctttaatc agacgattag tatcctggtg
     4021 atgggtaaag ttaccgccac ccgcccacca gatcattttg atattcggat acgtttgctc
     4081 tttaccgtta tgctgatatt tgccgccagg attttccagc gcatcgacga tacgggcaac
     4141 aggaatcgcc ctcattccgc catcatcagc ggcctcgctg gcctgtccgg caatcgccgc
     4201 agacatttcc ggcagcacgc cgccaacacg tgtcgggttg ccaccattgg agtaatgata
     4261 agagaaacca aaaccgccgc ccggtgtacc aatctgccct aacatcgccg ccagcgtcac
     4321 cagcatccag tgtttctgtt caccatattg ctggcgctga attccccagc ctgccatcag
     4381 cattgtgcga ttcgccgcca tcagttcagc gagtttgact atctgcgctt ccggtacgcc
     4441 agtaatttcc gccgcccagg ccgcgctctt cggcgtatta tcgctcttac ctctcagata
     4501 ctcttcaaac tgcggatacc cggtagtgta tttttcaagg aacactttat cgtgtttgcc
     4561 ttgtgtcatc agggtatgcg caatccctaa catcagtgcc acgtcggtgc ccatattcgg
     4621 cgcgatccag gtggcattat cgccaaagaa gtcgatggtt tcggagcgga taggatcaat
     4681 ggcaatcact ggtttgccag attttttcag ctgatggaag tattccagcc cttgctcatc
     4741 ggtactgctc caggcaattt ttaaggtatt cagcgggttc atcccccaca gcaccacaac
     4801 ctggctgttt tccagaatca gcggccagga ggtctgctgt tcatacacct ctacagaacc
     4861 gaccacatgc ggcatgatca cctgtgccgc cccggttgaa taatcgccgc tatgcccgga
     4921 ataaccgccc gccaggttca tataacgttg cagtaaggtt tgcgctttat gcaacacgcc
     4981 agaagagcgc cagccgtatg atccggcaaa aatagccgat ggtccgttag ctttgcgaat
     5041 gcgatcgtgt tgctcatgaa tcagttttaa tgcttgttcc cagctcacct gtacataggt
     5101 atcttcgcca cgacctttcg tcggttgcag tggattatcg agatagcttt ttctcaccat
     5161 cggatgctga atacgcgtcg tggtgtgtac ctgatccgcc gccgtagact gtaaggaatt
     5221 cggtatggtt ttcgccagcg cgcctgttga agaaacaatc ttgccgtcct tcacttctac
     5281 gttcatcgct ccccaacgtt ccgcggtgag gattttaccg tc
//
LOCUS       DAEEKQ010000221         5298 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.223, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000221 DAEEKQ010000000
VERSION     DAEEKQ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5298)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5298)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5298
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.223"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(513..889)
                     /gene="rnpB"
                     /locus_tag="KLK57_003869"
     ncRNA           complement(513..889)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="KLK57_003869"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(922..2067)
                     /gene="garK"
                     /locus_tag="KLK57_003870"
     CDS             complement(922..2067)
                     /gene="garK"
                     /locus_tag="KLK57_003870"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417593.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HBE0894118.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSVPVADG
                     GEGTVEAMIAATQGAERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESSATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            complement(2164..3054)
                     /gene="garR"
                     /locus_tag="KLK57_003871"
     CDS             complement(2164..3054)
                     /gene="garR"
                     /locus_tag="KLK57_003871"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HBE0894119.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLLLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            complement(3084..3854)
                     /gene="garL"
                     /locus_tag="KLK57_003872"
     CDS             complement(3084..3854)
                     /gene="garL"
                     /locus_tag="KLK57_003872"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HBE0894120.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRTNMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            complement(3870..5204)
                     /gene="garP"
                     /locus_tag="KLK57_003873"
     CDS             complement(3870..5204)
                     /gene="garP"
                     /locus_tag="KLK57_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HBE0894121.1"
                     /translation="MILDTVDVKKKGVHTSYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGGIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     VSTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
BASE COUNT         1280 a         1516 c         1257 g         1245 t
ORIGIN      
        1 gatggcaacc gcaaaaatgc ctgatgcgct acgcttatca ggcctacgcc atctctacaa
       61 tatattgaat ttgcgtgctt ttgtaggcag gataaggcgt tcacgcagca tccggcatga
      121 ataaagcgca ctcaacaatc tgaaacccgc cggaacggtt tattacgtac atcaggtgaa
      181 actgaccgat aagccgcttt cttttgggta tagtgtcgtg gacagtcatt catctttctg
      241 cccctccaaa agcaaaaacc cgccgaagcg ggtttttacg taaatcaggt gaaactgacc
      301 gataagccgc tttcttttgg gtatagcgtc gtggacagtc attcatcttt ctgcccctcc
      361 aaaagcaaaa acccgccgaa gcggtttttt acgtaaacca ggtgaaactg accgataagc
      421 cgctttcttt tgggtatagc gtcgtggaca gtcattcatc tttccgctcc tccaaaagca
      481 aaaacccgcc gaagcgggtt tttacgtaaa tcaggtgaaa ctgaccgata agccgggttc
      541 tgtcgtggac agtcattcat ctaggccagc aatcgctcac tggctcaagc agcctacccg
      601 ggttcagtac gggccgtacc ttatgaaccc ctatttggcc ttgctccggg tggagtttac
      661 cgtgccacgg actgttacca gccgcgcggt gcgctcttac cgcacccttt cacccttacc
      721 tgatcccgct tgcgcgggcc atcggcggtt tgctctctgt tgcactggtc gtgggtttcc
      781 cccccaggcg ttacctggca ccctgcccta tggagcccgg actttcctcc cctccgcccg
      841 tctcccccga agaggacgac gacgaagcgg cgactgtctg gtcagcttcg gcgcgcagta
      901 tagagggttt gcgcgccctt gtcaccccgc gttgcgcatt ccaatcgcca gtgtcgcggc
      961 gatattacgt gaagcacggc agatattgtc ataagccccg cggaatgctt cgtccaacgt
     1021 acctatgctg gtcaatacgc tgaagaccgc atcgatgcca tgctgatgta caacgccaac
     1081 atcatcggtc aggctacccg caatgccaat caccggttta tggtacttct tcgccacgtt
     1141 tgcaacgcca atcggtacct tgccgtgaat actctggctg tcaatacgcc cttcaccggt
     1201 gatcaccagc gtacaatcgt gaatatgttc ttccagattc agcgccgtag tgacgatttc
     1261 aataccactt ttcagttccg caccaagaaa cgccatcagc gccgcgccca taccgcccgc
     1321 agctcctgcg ccggggacat ctttcacatc aacatgcagc gcttttttaa tgacatcggc
     1381 atagtgagag aggttattgt ccagctcaac aatcatcgct tcactggctc ccttttgtgg
     1441 gccaaagatg cgcgatgcgc cgttatcgcc caccagcgga ttggtgacat cacaagcgac
     1501 gcgaatgacg caatctttta agcgcggatc gaggccggaa atatcaatat cattcagagt
     1561 attaagacta ccgccgccaa aaccaatttc attgccgttg gcgtcgcata atttcgcccc
     1621 cagcgcctgt accatgcctg cgccgccatc atttgtagcg ctgccgccaa tgccgataat
     1681 aatgtttgtt gcactgctct ccagcgcctg caggattaac tcgcctgtgc cgcgtgaagt
     1741 ggttacgagt ggatcgcgtt tttccgcagg taccagctcc agcccactgg ccgccgccat
     1801 ttcaataaac gcggttttgc catcgccgga gatcccccaa ctggcattca ctttctcgcc
     1861 cagcggccct gtaacccagg cgtgacgttc agccccctgg gtggctgcaa tcatcgcttc
     1921 caccgttcct tcaccaccgt cggcaaccgg aacagaaaca tactgtgcat caggaaaaat
     1981 ttcccgaaat cctttttcta tcgcctgcgc aacctcgctg gcagataaac tttctttata
     2041 agagtctggg gcgattacga ttttcatacc tatgcctgtt accacatgac gccggagggc
     2101 gtttctctta ttcggcctag attccaggcc cggattgcaa tacaccatcc gggcacgacg
     2161 tcattaacga gtaacttcga ctttcgccag tttttcgtag tagcacgcca gggcgctatg
     2221 atccgccgtt cctaaaccat ctgcacgcag tgcctgcatc atctccataa ccgcagctgt
     2281 gagcagcagt tgtgcgccga cgccgtgaga agtatccagc gcattcgcca gatccttaat
     2341 atgcagatca atgcggaagc ccggcttgaa gttgcggtcc atcaccatcg gcgctttggc
     2401 atccagcacg gtactgcccg ccagcccacc gcgaattgcc tgataaacca ggtccgggtt
     2461 aacgcccgct ttggttgcca gcgttaacgc ttctgacatc gcagcaatat tcagcgccac
     2521 aatgacctga tttgccagtt tggtgacgtt acctgcaccg atttccccgg tatgcaccac
     2581 ggaacccgcc atcgctttca tcaaatcata gtatttgtcg aaaatagcct tgtcgccgcc
     2641 caccatcact gacagcgtac cgtcgatggc tttcggttca ccgccgctca ccggagcatc
     2701 cagcatatca atgcctttcg ctttcagcgc ttcgctgatt tcacggcttg ccagcggtgc
     2761 gatagaactc atatcgatca ataccgtacc tggcttcgcg ccttcaataa tgccattctc
     2821 acccagcgcc acctctttca catgagggga gtttggcagc atggttatga tgacgtcgca
     2881 ctgttcagcg atcgctttag ccgtagacgc tgtttctgca cctgcagcaa tcacgtcagc
     2941 aatagcttct gggttacggt cagcaaccac cagcgagtaa cctgctttca gaaggttttt
     3001 actcattggt ttacccataa tccccaggcc aataaaacca actttcatag tcatatcaac
     3061 catctctctt gttgcggtgg tggttatttt ttaaaggtat cagccagttt ctgagtggca
     3121 gagcggaaga cgccaagatc gctgccgaca gccacaaacg tcgcgcccca ttccagataa
     3181 cgacgcgcat cggcttcgac cggcgcgaga ataccgctgg gtttgccgtg cgcgctggca
     3241 cggttaaaaa tatgctgaat tgctttttgt acatccggat gcgaggcatt gccgagatgg
     3301 cccaatgccg cagccagatc gctggggccg acgaagatac cgtctacacc ttcggtagcg
     3361 gcaatagcat cgacgttatc tacgccctgc tgactttcta tctggaccag aatagtaatg
     3421 ttcttgttcg actgagcgaa ataatccgcc acggtgccaa acatattggt gcggtgagaa
     3481 acggagacgc cgcgaatgcc ttccggcggg taacgagttg atgccaccgc ctgctctgct
     3541 tcctcttttg tttctacaaa aggaatcagg aagttataga aaccgatatc cagaagacgc
     3601 ttaataatta ccggctcgtt ggtcggcact cgcactactg gcgcgctggc gctgcctttc
     3661 agggccatta actgcggaat aaacgtggaa atatcgtttg gcgcatgttc gccatccagc
     3721 accagccagt caaacccagc caaaccaaga acttcagtgc taatcgggtt agagagtgct
     3781 gaccagcaac caatttgtac ctgtttcgca gccagtgcgg ctttgaattt attcgggaaa
     3841 acatcgttat tcatcgctta tacctttgct tatttctgca attccatacg tttaatgtcg
     3901 ccaactacga agaggtagca gaccatcgcc atcagcgctg aacatcccac gaaaaccagt
     3961 gctgcattga aggagtgcag ttcacttacc aggtagccaa tcaccagtgg agtgacaatg
     4021 gaggcaacat tgccaaagac gttaaagacg ccgccacaga ggccaacaat ctctttcggc
     4081 gcggtgtcag aaatcaccgg ccagcccaga gcaccaaatc ctttgccaaa aaaagccagc
     4141 gccatcagca tgaccaccag cgtggtgttg ttggtgtagt tacataagat gatggtggaa
     4201 accagcaaca ttcccagcac aatcggtagc ttacgtgcca gggtcaggga taaaccgcgt
     4261 ttgatcagat aatccgagaa gacacctccc agcacgccgc ccgcaaaacc acacagtgcc
     4321 ggaatcgagg cgaccagacc cactttcaga atcgacatgc ctttttcctg caccagataa
     4381 atcgggaacc aggtgaggaa gaaccaggtg atggtgttga taaaatattg tccgaaaaat
     4441 acgcccaaca tcatgcggtt agagagcaat tgcttgatgt aatgcagttt gggaccgctt
     4501 gctgccgcgc tgcccggctt tttgtggtcc atatcgacca ccgcgccatt ttcagagata
     4561 aacttcagct cttccgcaga catacgtggg tgatctgtcg ggttatgaat caacttgatc
     4621 cacagcgccg tcagcacaaa accaatcccc cccataacgg tgaagacgtg ctcccagccc
     4681 caagcgaaag tcagccagcc aagcagcggc gaaaagagcg ccagcgagaa atattgcgcc
     4741 gagttaaaga tggcggaggc agtaccacgt tctttcgtcg ggaaccaggc ggcgacaata
     4801 cgggcgttcg ccgggaatga tggcgcttcc gagaacccca gcataaagcg cataaagaac
     4861 atggagatcc ctgcccaggc cagcgggaac atatcaacaa agccttgcag gaaggtgaac
     4921 agcgaccaga aaaagaggct gtaggtgtaa acttttttcg agccaaactt atcaagcaac
     4981 cagccgccgg ggatttgcat cagcaagtag gcccagccaa aagcagagaa gatgtaaccc
     5041 atcgaaaccg cacttaactg caactctttt gccacttcgg taccagcaat agacagcgtt
     5101 gcacgatcgg cgtagttaac ggcggtaaca ataaaaataa tcagtaatat taaatagctg
     5161 gtatgcacgc ctttcttttt tacgtcaacg gtgtccagaa tcattttatt tacctcgggt
     5221 acttatgctg atttttatta ttatggggaa ggtgttattt atgagtttta tttatgccgt
     5281 tacgacaatg aactcggg
//
LOCUS       DAEEKQ010000222         5281 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.224, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000222 DAEEKQ010000000
VERSION     DAEEKQ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.224"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..471)
                     /locus_tag="KLK57_003874"
     CDS             complement(<1..471)
                     /locus_tag="KLK57_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0894122.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIG"
     gene            complement(471..1391)
                     /locus_tag="KLK57_003875"
     CDS             complement(471..1391)
                     /locus_tag="KLK57_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0894123.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITFDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            1529..2428
                     /gene="pgrR"
                     /locus_tag="KLK57_003876"
     CDS             1529..2428
                     /gene="pgrR"
                     /locus_tag="KLK57_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HBE0894124.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            2765..4378
                     /gene="mppA"
                     /locus_tag="KLK57_003877"
     CDS             2765..4378
                     /gene="mppA"
                     /locus_tag="KLK57_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HBE0894125.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="KLK57_003878"
     CDS             complement(4429..>5281)
                     /gene="ynaI"
                     /locus_tag="KLK57_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HBE0894126.1"
                     /translation="INYVIENYKLKFITPDVIDFICTSLIAVILTIKLFLLINQFEKQ
                     QIKKGRDITSARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKD
                     ILSNFFSGIMLYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSL
                     FSSISVENPGRMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYF
                     NQFADSSLNIMVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNI
                     TPPEQGR"
BASE COUNT         1429 a         1202 c         1226 g         1424 t
ORIGIN      
        1 gccaatcgga tcgtaattgc tgaaaccttc ccgaaaaaga cgcccgatat taacacccgt
       61 aatactcact actgctttaa ttcgtttttc tgtcagcgcg gcattaatgg catagccacc
      121 acccccacac actcccagca cgccaatacg ttcagcatta acatatggca gagttactgc
      181 ataatcgata acacggctaa tatcctctac gcgctgtgtc ggatcttcta tccagcgagg
      241 ctccccacca gattctccct ggaaactggc gtcataagcg agaacgagat agcctttttc
      301 agccaatgcc ttgccataga tattgccaga cgtctgctcc ttacaactgc caaaaggatg
      361 gacgctgata atcgccggat actgttttga ctcatcaaaa tttggtggaa actgaatatt
      421 ggcagcaata tgccaggcca tatcactatt gcggatttga atatgtttca ttacagagtt
      481 tcctcaaaga acggagctaa tactgaaatt gcttcagcca cataggcttt gccgtcataa
      541 agatccatat ggtttgcgcc ttcaacaatg tgatagcgtt tatcttggct tgatgctcga
      601 tcgtacaggt cgtcactcat ccatttgctc cctgcctggc tgcccgccac aatctgcatc
      661 ggctgagtca ggtacacttc cgccatatgg taagcatcaa aggtaataat ctggttaagg
      721 ctgcgcaaag tagcgtaacc cggtgctgtt ggatactgcg cgcgaggggt gtggtaatac
      781 tcccaggcct gacgcagttc ttcgttcggc gcatcggact ccttcattgg tgccagtggc
      841 ataacggcat attctccgct gctaatatcg ctggttcggg cgtttgaacc cgcttcaacg
      901 tatggcaggg catcaataga tttcacattg ttttcccaac cattacggaa catcgaacca
      961 atattgaccg cactaacggt accgatggcc ttgatgcggc gatcctgaat tgcagcattg
     1021 gctgtatatc ctgcaccggc acaaattccc atcgcaccaa ttcgggtatt gtcgacataa
     1081 gaaagcgttg tcaggtaatc aatcacggca ctgatgtctt cagtacgaat gtatgggttt
     1141 tctaactgac gcggctcgcc gccactttca ccttgataag atgcgtcata agcaatagtg
     1201 acaaatccct tttccgccag ttttttggca taggttccgg ccgtttgttc tttaacgccc
     1261 ccacctgggt gagataccac aattgcctga tactgacggg tttcatcaaa ttttggaggg
     1321 aaatagatca ctgcagacaa agagatggtt ggattattgc tgttagtgaa gctgacttta
     1381 ttattcatca ttccgttcct catggagttg tcggttcgtt ttaatggttg gtgatatcac
     1441 tatagatatt gatcattaag ttgattagac ccaaaattat gattagacct atcatttaaa
     1501 tgattaatag attgcatgga gatactgaat gaagcgtgaa gaaatcgctg atctgatggc
     1561 gtttgtcgtc gttgcagagg agcgtagctt cactcgtgca gcagcccgcc tgagcatggc
     1621 gcagtcagct ttaagccaga tagtgcgtcg tatagaagaa cgattgggat tgcggcttct
     1681 gacgcgaacc acgcgcagcg ttgttccaac tgaagcgggc gagcatcttt tgtctgttct
     1741 tggccctatg ttgcatgaca tagattcagc cctggcatcc ctgagcgagc tgcagaaccg
     1801 cccatccggg acaatacgta ttactactgt agaacatgca gccaaaacga tattgttacc
     1861 agcaatgcgc acattcctga aatcgcatcc tgaaattgat attcagctca ccattgatta
     1921 tggtttgacc gatgtcgttt ctgaacgttt tgatgcaggc gtccgtctgg gtggggagat
     1981 ggataaagat atgatcgcca ttcgaatcgg gccagatata ccaatggcta ttgttggctc
     2041 accggattat ttttctcgcc gaagtgttcc aacgtcagtg tcacaattaa tagatcatca
     2101 ggcaattaat ttgtatcttc ccacatcggg tacagcaaat cgctggagat taatacgcgg
     2161 tggacgtgaa gttcgtgttc gcatggaagg tcagctttta ctgaatacga tagacctgat
     2221 cattgatgct gcaattgatg ggcatggatt ggcgtatcta ccttatgatc aggttgagcg
     2281 ggctattaaa gaaaaaaaac tgatacgtgt tttggataaa ttcacaccag atttacccgg
     2341 ttatcacctg tactatccac accgtcgaca tgctggctcg gcattctcat tatttataga
     2401 taggctgaag tataaaggtg ctgtttagca ctacttgctg atacattaat ttaatttttc
     2461 tcttaacgta ttctcagttc ctttcaacgt tttggttatt ttttattctt cgtacaatgg
     2521 cgacagatgc tgattatgat accgaaaact ggtttgaacg tgcgaagcct gaacgtagtg
     2581 ttcggtgttc tatgtgcttt accgcatttt gtcgtctatt atttacacta aatctgattt
     2641 aatatattga tacataaaac attttatgct ccctgtgtca cttttttgat ggaagttgtt
     2701 tgcatttctt taaggcgaaa caaataatta cgcatcaatt ttaatgtcgg ttagagggaa
     2761 acttatgaag cactctgttt cagtcacgtg ttgtgcgctg ttggtcagca gcatttctct
     2821 ttcgtatgct gcagaagttc cgagcggcac agtactggca gagaagcagg agctggtgcg
     2881 ccacattaaa gatgagcctg cgtcgctgga tcccgctaaa gccgtgggcc tgccagagat
     2941 tcaggtcatt cgcgatctgt ttgaaggact ggtgaatcag aacgagaaag gggagattgt
     3001 ccccggcgtt gcgactcagt ggaaaagtaa tgacaaccgt atctggactt ttaccctgcg
     3061 cgataacgca aaatgggcgg atggcacacc ggtaacggcg caagattttg tctacagctg
     3121 gcagcgtctg gtggacccga aaacgctatc gccatttgca tggtttgccg cgctggcggg
     3181 aatcaacaac gcacaggcga ttattgatgg taaagctacg ccagaccagc ttggcgtcac
     3241 cgcagttgat gcccatactt tgaaaattca gcttgataaa ccgttgccgt ggtttgtgaa
     3301 tttaaccgct aactttgcct tcttcccggt gcaaaaagcc aacgtagaaa gcggtaaaga
     3361 gtggacgaaa cccggaaatc tgatcggcaa tggcgcttat gttcttaaag atcgcgtagt
     3421 caatgaaaaa ctggtcgtgg taccgaatac ccattattgg gataacgcca aaacggtact
     3481 gcaaaaagtg accttcctgc caattaatca ggaatccgca gccactaagc gttacctcgc
     3541 gggggatatt gatatcaccg aatccttccc gaaaaatatg tatcagaagc tgttgaagga
     3601 tattccgggg caggtttata cgccgccgca gctcgggacc tattattatg cgtttaacac
     3661 gcaaaaaggg ccgacggcag atcagcgcgt tcgtctggca ttaagtatga cgatagatcg
     3721 ccgcctgatg accgaaaaag tattagggac gggcgaaaag ccagcgtggc attttacacc
     3781 agatgttacc gccggattta cgccggaacc ttcgccgttt gaacaaatga gtcaggaaga
     3841 actgaatgcg caggcaaaaa ctttgttgag cgcagctggt tatggtccgc aaaaaccgct
     3901 gaagctgacg cttttgtata acacttcaga aaaccatcaa aaaattgcga ttgctgtagc
     3961 atcgatgtgg aaaaagaacc ttggcgtaga tgttaaattg caaaatcagg aatggaaaac
     4021 ctatatcgat agccgtaaca ccggcaattt tgatgttatc cgcgcctcat gggtggggga
     4081 ttataatgaa ccctccactt tcctgacatt attaacgtca acgcattcag gaaatatttc
     4141 acgctttaac aatccggcat acgacaaagt tctggcccag gcatcgacgg aaaataccgt
     4201 taaagcgcgt aatgccgatt acaacgcggc agaaaaaatc ctcatggagc aagcaccgat
     4261 tgcaccaatt tatcaatata ccaatggacg attaatcaag ccgtggctga aaggttatcc
     4321 cattaataac ccggaagatg tggcgtacag ccggactatg tatattgtga agcattgatg
     4381 tgatgggaac tggcgttacc cttgtggata acgccagtga tgtctgattt agcgcccctg
     4441 ttccggcggt gtaatgttat ccatatacag cgtctggctg gggaaggcaa agtccgcgcc
     4501 gtgtgactgc acaatatcga taatcttcaa ataaacgtct tgctgtgcag caagccattc
     4561 agcccatacc gtggttttgg taaagcaata aaccataata ttcaatgacg agtcggcaaa
     4621 ctggttgaaa taaaccagta aggtttgtcg ctggtcgatg gccgggtgat ttttcagcat
     4681 ctcacgtacg gcttcgacaa taacgcccac ttttgccgca tcctcataac gtaaaccaat
     4741 ggtcgtggta atgcggcggt tggtcattcg tcctgggttt tctacgctga tcgacgaaaa
     4801 cagcgagttc ggtacgtaca atggacgatt atcaaaggtc gtaattttgg taattcgcca
     4861 gccaatttcc gctactgtac cttcgatatt tctgtccggt gaacggatcc agtcgccaat
     4921 actgaaagga cggtcgaaat agagcatgat cccggaaaag aaattactca gaatatcttt
     4981 accggccata ccgacagcca gaccaccaat accaccaaag gtcagcaagc cagaaaggct
     5041 catgccgaaa tgttcgccat aaagcagaac aagcaccaca ataatggtga ttttgataat
     5101 gcgcgacata atccgcgcac tggtgatatc gcgacctttt ttaatctgct gtttttcaaa
     5161 ctgattaatc agcaaaaata gcttaatcgt cagaataacc gcaatcaggg acgtacagat
     5221 aaaatcgata acgtctgggg tgataaattt gagtttatag ttttctataa cataattaat
     5281 a
//
LOCUS       DAEEKQ010000223         5278 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.225, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000223 DAEEKQ010000000
VERSION     DAEEKQ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5278
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.225"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(41..193)
                     /gene="hokA"
                     /locus_tag="KLK57_003879"
     CDS             complement(41..193)
                     /gene="hokA"
                     /locus_tag="KLK57_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HBE0894127.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     ACNLKE"
     gene            complement(519..2588)
                     /gene="glyS"
                     /locus_tag="KLK57_003880"
     CDS             complement(519..2588)
                     /gene="glyS"
                     /locus_tag="KLK57_003880"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HBE0894128.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLDAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2598..3509)
                     /gene="glyQ"
                     /locus_tag="KLK57_003881"
     CDS             complement(2598..3509)
                     /gene="glyQ"
                     /locus_tag="KLK57_003881"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HBE0894129.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3604..3903)
                     /locus_tag="KLK57_003882"
     CDS             complement(3604..3903)
                     /locus_tag="KLK57_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HBE0894130.1"
                     /translation="MMMNAFFPAMALMVLVGWSTPSPVQKAQRVKVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            4078..5073
                     /gene="wecH"
                     /locus_tag="KLK57_003883"
     CDS             4078..5073
                     /gene="wecH"
                     /locus_tag="KLK57_003883"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HBE0894131.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMAVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(5115..>5278)
                     /locus_tag="KLK57_003884"
     CDS             complement(5115..>5278)
                     /locus_tag="KLK57_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894132.1"
                     /translation="GLWNATLLLSEKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKET
                     QVTQEEYSE"
BASE COUNT         1242 a         1363 c         1304 g         1369 t
ORIGIN      
        1 cgccgtaaga aaggcgggaa taacttcccg ccaaagactc ttactctttc aaattgcagg
       61 ctaaaaatgc cgccagctca taactctcct gtttaatatg caattcacac agtgaatctc
      121 ttatcatcca ggtgaaaaat aaaagcgtga aacaaatcac tattaaagaa agtaatctat
      181 atttctgcgg cattcccagc ctcctgtgtt gatttccaac gagtaagagg ctaaccttat
      241 ggtgttcagg catagggtag cctcactttg atttatagtc aggtggggct tttctctgtc
      301 tgcctttcgg tgaatacctg agacaaacag tctcaagcac ccgtggctat tctagcttaa
      361 taagtttgtt tcttctcctt gatataatcc taaaaaaacc tcataaaatt aatatatgag
      421 ataatcttta ttcaacagaa gattattaaa ggttgctgta ttatttagcg ataataaaaa
      481 aagcctgcca gatggcaggc tttttaataa cggcgttatt actgcaacag cgaaatatcc
      541 gcaacgcgca ggaacagttc gcgcagtttc tccagcatgg tcagacggtt gagacgcaat
      601 tctttgtcat caaccatgac catcacttta tcgaagaaag catcaaccgg ctcacgcagc
      661 tctgccagtt cgaccagcgc atcctggtaa cgaccttccg caaagtacgg ctccagcttg
      721 tcacgtagca ccacaacctg catcgccagt ttaatttctt ccggctcttt cagggtagag
      781 gcattcacgc ggtcgctcag cacttcgtca gatttcgcca gaatgttaga tacacgtttg
      841 ttcgccgccg ccagtgcagc agctgcatcc agggtacgga aatgcgatac cgctttcata
      901 cgggcatcga aatcagccgg acgagtcgga cgacgcgcca gtaccgcctg gatggtgtca
      961 acggtgtaac cttcgtcctg ataccaggcg cggaagcgac cgagcataaa gtcgataaca
     1021 tcatcaacta cgttggcgtt agtcagctta tcgccataca gacgcactgc ttcttcggtc
     1081 agcgtttgca gatcaaggtt gaggttcttc tcaacgataa ttcgcagcac gccaagtgcg
     1141 gcacgacgca gcgcaaacgg gtctttgtcg cctttcggat gctgaccgat accgaagata
     1201 cccgccagag tatccatctt atcagcaatc gccagcgcac aagctaccgg gttggacggc
     1261 aggtcatcac cggcaaagcg cggctgatac tgctcattca gcgcgacggc tacgtcttcc
     1321 gcttcgccat cgtgacgcgc gtagtgcatc cccataacgc cttgggtgtc ggtgaactcg
     1381 aagaccatgt tggtcatcag gtcacactta gacagcaggc ccgcacgggt tgcgtggtta
     1441 acgtcagcgc caatctgttc agcaatccag ccagccagcg cctggatgcg gtcagttttg
     1501 tcacgcagcg tacccagctg ttgctggaac agcacggttt gcaggcgcgg caggttatct
     1561 tccagacgtt ttttacggtc ggtattgaag aagaactcgg catccgccag acgtggacga
     1621 acgactttct cgttaccaga gataatttgc tgcggatctt tcgattcgat gttggcaacg
     1681 aagatgaagt tcggcagcag tttgccatcg ttcgcataca ccgggaagta tttctggtca
     1741 cctttcatgg tgtaaaccag ggcttcagcc ggcaccgcga ggaatttctc ttcgaatttt
     1801 gcggtcagaa cgaccggcca ctccaccagc gaagccactt cttccagcag gctttcgctt
     1861 aagtcagcgt taccgccaat cttacgcgct gcttcttcgg catcggcttt aatcttcgct
     1921 ttacgttctt cgtaatcggc gatgactttc ccgcgctcac gcagaatttc cggatactga
     1981 tcggcgttgt cgatggtgaa ttccggttcg cccataaagc ggtggccgcg aatcacgcga
     2041 tcggactgaa tgcccagaat ggttgccgga atgactttgt cgcccagcag cagggtcacg
     2101 gtgtgcaccg gacgcacgaa gtgcacgtcg cttgcgcccc aacgcatcag tttcgggatc
     2161 ggcagtttcg ccagcgaagt cgcaaccata ttcggcagta gtgcttcagt gctttcgccc
     2221 ttcacatggg cgcgatacag cagccattcg cctttatcgg tagtcagacg ctcagcctgg
     2281 tcaacggtaa taccgcaacc acgcgcccaa ccttctgccg ctttgctcgg tttgccttca
     2341 gcgtcgaacg cctgggcaat cgccgggccg cgtttttcga tttcacgatc tggttgcgct
     2401 tccgccaggt tagctacttt caacgccaga cgacgcggag cagcaaacca ttgaacggtg
     2461 ccgtgtgcga ggccagcgtt atccagctcc gcagtaaagt tcgcagcaaa ggactcagcc
     2521 aggctgcgca gtgcttttgg tggcagctct tcagtgccga tttccaccag aaaagttttc
     2581 tcagacatag ccgcctctta cttatctttg ttgcacatcg ggaagccgag ggcttcacgg
     2641 gaagcgtagt atgcttctgc cactgctttg gtcagggtgc gaatgcgcag aatgtagcgc
     2701 tgacgctcag tgacggagat ggctttacgc gcatccagca ggttgaagct gtgggcggct
     2761 ttcagaatac gctcgtaggc tggcagcggc agcggatttt ccagcgccag cagctgctgc
     2821 gcttctttct cgtactgctc gaagcaggtg aacaggaaat ccacatccgc gtattcgaag
     2881 ttgtaagtgg actgctccac ttcgttctga tggaacacgt cgccgtaggt ggttttaccc
     2941 agcgggccgt cgctccagac caggtcgtaa acgctgtcta cgccctgaat gtacatggcc
     3001 agacgttcca gaccgtaggt gatctcgccg gtaaccggtt tacactccag accaccaacc
     3061 tgctggaagt aagtgaactg cgtcacttcc atgccgttta gccacacttc ccagcccagt
     3121 ccccaggcac ccagagtcgg gttttcccag ttatcttcca cgaaacggat gtcgtgaata
     3181 gtcgggtcca tgcccagctc tttcagagaa ccgaggtaca gctcctgaat attgtccggc
     3241 gatggcttaa tgaccacctg gaactgatag tagtgctgta aacggttggg gttttcgccg
     3301 tagcgaccat cggtcgggcg acgagaaggc tgaacataag cagccgccat cggttctggc
     3361 cccagcgcgc gcagacaggt cattgggtga gaggttcccg cgccgacttc catgtccaat
     3421 ggttgaacaa tggtgcagcc ctggcgagcc cagtaatcct gtaaggtcag gatcaagccc
     3481 tggaaggtcc tggtatcaaa cttttgcata ttatttcgtg ctggatacgt gtggatttaa
     3541 aggaagggat cagtataccc gctggatgga agatatacag tacgaaacgg gaaaaagcag
     3601 ggcttaacgc atggaaagat gcaaaaaatg gccatctgcg tcaaaagaac agacaaactg
     3661 ctctttacgg aaggtataac cgcgcatttc atagcttccc tggaactgtt cgaaggcggt
     3721 gacgtcgatt ttttgctcgc cggtgttata acgttttgcc gcctgatcct tgcataacgc
     3781 ttccatattc aacgaacgca gaggatcaac ctttacccgt tgtgctttct gcacgggtga
     3841 cggtgtagac caacccacta gcaccataag cgccattgcc ggaaagaaag cgttcatcat
     3901 cattaaatta ccgtctggtt tgcaggtgag tcttattatt tatatcggta tagtggctga
     3961 tatcttacga atttccttaa gcctctataa gctgtagtga taaaactcag attattccag
     4021 tgcgccaacc aaatgattta tcggtcagac tttttttaac cagatacaga gacacagatg
     4081 cagcccaaaa tttactggat tgataacctg cgagggatag cgtgtttaat ggtggtgatg
     4141 attcacacca ctacctggta tgtgaccaat gctcatagtg ttagccccgt cacatgggat
     4201 atcgccaatg ttctgaattc tgcctctcgt gtcagcgtgc cgctattttt catgatttcc
     4261 ggctatctct tttttggcga acgcagcgcc cagccgcgcc atttcttgcg tatcggctta
     4321 tgtctgattt tttatagcgc aatcgcactg ctctacattg cgctgtttac ctccatcaat
     4381 atggagttag cgctgaaaaa cctgctgcaa aagccagtgt tttaccactt gtggtttttc
     4441 ttcgcgattg cggtgattta tctggtttca ccgctgattc aggtgaagaa cgtcggcgga
     4501 aaaatgttgc tggtgctaat ggcggtgatt ggcattatcg ctaacccaaa cacagtgccg
     4561 cagaaaattg acggttttga atggctgcca attaacttat atatcaatgg cgatactttt
     4621 tactacattc tgtatggcat gttgggccgc gctataggga tgatggacac acagcataaa
     4681 gcactgtcgt gggtgagcgc cgcgctgttt gcgacggggg tttttattat ctctcgcggg
     4741 acattatatg aattgcagtg gcgcggaaat tttgccgata cctggtatct ttactgtggg
     4801 ccgatggttt ttatctgcgc aatcgcgcta ttgactctgg ttaaaaacac gctggatacg
     4861 cgtaccattc gcggacttgg cttaatctcc cgccattcat tgggtatata cggattccac
     4921 gccttgatta tccatgcgct gcgcacccgg ggaattgagc ttaaaaattg gccaatactg
     4981 gatattattt ggatcttttg cgcgacgttg gcagcgagtt tgttactttc tatgctggta
     5041 caacgaatcg acagaaacag attagtgagt taagtaaaag cccgttcaca ttggactgac
     5101 cgggcttacg tgaattattc gctgtattct tcctgggtaa cctgtgtttc ttttggcggg
     5161 tttattccgg catcacgaat attcttctgc accgctactg caccaaacaa gcttaagaag
     5221 aaagccagtc cataaaaacc tttttcactg agtaataatg tcgcattcca cagaccta
//
LOCUS       DAEEKQ010000224         5258 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.226, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000224 DAEEKQ010000000
VERSION     DAEEKQ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.226"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..362
                     /locus_tag="KLK57_003885"
     CDS             <1..362
                     /locus_tag="KLK57_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="acts in anaerobic beta-oxidation of fatty acids;
                     possible ligase/synthase involved in anaerobic growth on
                     fatty acids; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cyclohexanecarboxylate-CoA ligase"
                     /protein_id="HBE0894133.1"
                     /translation="TGRKKDIIVRGGENISSREVEDILLQHPKIHDACVVAMPDERLG
                     ERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKYPEHIVVIEKLPRTASGKIQKFLL
                     RKDIMRRLTQDVCEEIE"
     gene            complement(419..2797)
                     /gene="ppsA"
                     /locus_tag="KLK57_003886"
     CDS             complement(419..2797)
                     /gene="ppsA"
                     /locus_tag="KLK57_003886"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HBE0894134.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHEISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVEMMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            3130..3963
                     /gene="ppsR"
                     /locus_tag="KLK57_003887"
     CDS             3130..3963
                     /gene="ppsR"
                     /locus_tag="KLK57_003887"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HBE0894135.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            4120..5166
                     /gene="aroH"
                     /locus_tag="KLK57_003888"
     CDS             4120..5166
                     /gene="aroH"
                     /locus_tag="KLK57_003888"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HBE0894136.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGAATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYAPRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLLEGTQKIVGSQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
BASE COUNT         1292 a         1402 c         1288 g         1276 t
ORIGIN      
        1 ttaccggacg caaaaaagat attattgtcc gcggcggcga aaatattagc agccgtgaag
       61 tagaagatat tttattgcag catcctaaaa ttcacgatgc ctgtgtggtt gcaatgcccg
      121 atgaacgttt aggtgaacga tcatgcgctt atgtcgtgct gaaagcgccg catcattcat
      181 tatcgctgga agaggtggtg gcttttttta gccgtaaacg ggtcgcaaaa tataaatatc
      241 ctgaacatat cgtggtaatc gaaaaactac cgcgtactgc ctcaggtaaa atacaaaagt
      301 ttttgttaag aaaagatatt atgcggcggt taactcagga tgtctgtgaa gagattgaat
      361 aagtttcatc ttcggggatc acataacccc ggcgactgaa cgccgccggg gatttatttt
      421 atttcttcag ttcagccaag cttaaccagg tttgcaccac ggtgtccggg ttcagagaca
      481 ggctatcgat cccctcttcc atcaaccatg cggcaaagtc ttcgtggtcg gacggaccct
      541 gaccgcaaat cccgacatat ttgccctgtt tcttcgcggc acggatagcc atcgacagca
      601 gtgctttcac cgcatcgttg cgctcatcga acaattcaga caccacgccg gagtcacggt
      661 ccagaccgag cgccagctgc gtcatatcgt ttgagccaat tgagaagccg tcgaaatatt
      721 cgaggaactg ctcggccagc aaggcgttgg acgggatttc acacatcatg atgattttca
      781 gcccgttctc accacgcttc agcccctgac gcgccagttc ctcaaccacc gctttcgcct
      841 gatctacggt acgcacgaac gggatcatga tctcaacgtt ggtcagcccc atgtcgttgc
      901 gcacacgttt cactgcttcg cactccagcg cgaagcagtc gcggaagctg tcggaaacat
      961 agcgtcccgc gccacggaag ccgagcatcg ggttctcttc atctggctcg taacgctcac
     1021 caccgaccag gttggcatat tcgttcgatt taaaatcaga gagacggaca atgacgcgct
     1081 tcggataaaa cgcggcaccc agcgtcgcga tcccttcagt cagacgacca acgtaaaatt
     1141 cacgcggaga atcaaaacct ttcatcatct cgcggatttc attttgcaac tgcggttcct
     1201 gatcgtcaaa ctcaagcagt gcgcgtgggt ggacgccaat catacggttg atgataaatt
     1261 ccagacgcgc aaggcccacg ccttcgttcg gcaggcaggc gaagtcgaaa gcacggtccg
     1321 ggttaccgac gttcatcatc actttcaacg gcagatccgg catcatttct acgctggagc
     1381 ttttcacgct aaattccagc aactccgcat agacgtaacc ggtatcacct tcggcacaag
     1441 aaacagtgac gttctcaccg tctttcatcc gttctgttgc atcaccacag cccactaccg
     1501 ccggaatgcc cagttcacga gcgatgatcg ccgcgtgaca ggtacgaccg ccacggttgg
     1561 tgacgatggc agatgctttc ttcatgatcg gttcccagtc cgggtcggtc atgtcagtga
     1621 ccagcacgtc accaggttcg atgcggttca tttcgctgat ctcatggatg actttcaccg
     1681 gacccgcgcc gatgcgatga ccgatagcac ggccttcggc gataatctta ccctgtgaat
     1741 gcagcgtata acgctccatg acctgaccgc gtgagcgcac ggtttccgga cgcgcctgca
     1801 caatgaacag tttgccagtg tggccatctt tcgcccactc aatatccatc gggcgaccgt
     1861 agtgtttctc aatttgtacg gcctgttttg ccagttcctg cacttcttcg ttggtcagcg
     1921 agaagatgtc acgctgttcc tgcggtacgt cttcgatttt aacctgcttg ccgtgctcct
     1981 gggtcggcgc gtaaaccatg cggatttttt tcgaccccat ggtgcgacgc acgatagccg
     2041 ggcgattcgc cgccagtgtc ggtttatgga cgtaaaactc atccgggtta accgcaccct
     2101 gcacgaccat ttccccaagg ccccatgcgg aagtgataaa caccacctgg tcaaagccgg
     2161 attcggtatc aatggagaac atcacgccag aagatgcgag gtcggagcgc accatccgct
     2221 gaacaccggc agagagcgcc acgccacggt gatcgtaacc ctggtgcaca cgataagaga
     2281 tggcgcgatc gttaaacaga gaagcaaata catgcttcac tgccacgaga acggcgtcaa
     2341 aaccctgaac gttgaggaag gtttcctgct gaccggcaaa agaagcgtcc ggcatatctt
     2401 ctgcggtggc ggaggagcgc accgcaaaag aggcgttttc gtcatcggcg gaaagctgtg
     2461 cataggcttc gcggatggcg ttttccagct caggctggaa gggagtgtcg ataatccact
     2521 ggcggatttg cgcgcccgct ttcgcaagct gagtaacatc gtcaatatcc gttttatcca
     2581 gcagttcata aatgcgctgg tttacgccgc tttggtccag aaactggtta aacgcgtcgg
     2641 cggttgtggc gaaaccattc ggaacggaaa cacccattcc ggaaaggtta gtaatcattt
     2701 cacccaggga ggcatttttg cccccaaccc tgtctacatc attcatgccg agttggttat
     2761 accaaagcac cagcggtgac gagccattgt tggacatcga acaatccttt tgtgataaat
     2821 gaacggtttg agaaacacat ttctgcgcat ttatctttgc atatttaacc ggatgaaaaa
     2881 aacggtgaat cgttcaagca aatatatttt tttacttttt aagactgatc ccagcgttgc
     2941 gcaaatctgc tctcttcgac aatttccaca taaaccatcg gtataaacga aaccataaaa
     3001 atgaaatgct gttttcataa aaaataaaat tgaaggttca ttttataaac cagacataac
     3061 gtcctacgct tctgtgcgtt tttaatttat gctttcatag aattatgtct gcatcacggg
     3121 aagaacaaaa tggataatgc tgttgatcgc cacgtttttt atatttctga tggtacggca
     3181 ataactgcgg aggtattagg acacgcagta atgtcacaat ttcccgtcac tatcagcagc
     3241 attacgctgc cgtttgtcga aaatgagagc cgtgcacggg cagtgaagga tcagattgac
     3301 gcaatttatc accagacagg tgtgcgcccg ctggtcttct actccatcgt gttgccggag
     3361 attcgcgcca tcatcttgca aagtgaaggc ttttgccagg atatcgttca ggcgctggtt
     3421 gccccgctac aacaagagat gaaactggat ccaacgccga ttgctcatcg tacccatggc
     3481 cttaacccta ataatctcaa taaatatgat gcgcgcattg cggcgattga ttacaccctc
     3541 gcccacgatg acggcatttc gttgcgcaat ctggatcagg ctcaggtgat cctgctcggt
     3601 gtttctcgct gtggtaaaac ccccaccagt ctttatctgg caatgcaatt tggtatccgc
     3661 gcggcaaact acccctttat tgccgacgat atggataatc tggtgctacc cgcgtcgctc
     3721 aaaccgcttc agcataaatt gttcggcctg actatcgacc cggaacgtct ggcggcgatt
     3781 cgcgaggaac gtcgggagaa cagtcgctat gcctcgcttc gtcagtgcag gatggaagtc
     3841 gcggaagtgg aagcgttgta ccgtaaaaat cagatcccgt ggattaacag taccaattat
     3901 tcggtagaag agattgccac caagatcctc gatatcatgg gccttagtcg ccgaatgtac
     3961 tagagaacta gtgcattagc ttattttttt gttatgatgc taaccacccg gcgaggtgtg
     4021 acacacctcg cacttgaaat cagcagcgat tggtttatcg tgatgcgcat cacttcccgg
     4081 cagtcctgcc gtagaagcaa caaatttctg agacttgtaa tgaacagaac tgacgaactc
     4141 cgtactgcgc gtattgagag cctggtaacg cccgccgaac tcgcgctacg gtatcccgta
     4201 acgcctggcg ccgccaccca tgtcaccgac tcccgccgca gaattgaaaa aatactcaat
     4261 ggtgaagata agcgactgtt ggtcattatt ggcccctgct cgatccacga tctcaccgct
     4321 gcaatggagt acgccccccg tctgcagtcg ctgcgcaacc agtaccagtc acggctggaa
     4381 atcgtaatgc gcacctattt tgaaaaacca cgaactgttg tcggctggaa aggactaatc
     4441 tccgatccag atttaaacgg cagctatcgg gtaaatcacg gtctggagct ggcgcgcaaa
     4501 ttacttttac aggtaaatga gctgggcgtc ccaaccgcga ctgagttcct cgatatggtg
     4561 accggtcagt ttattgctga tttaatcagt tggggcgcga ttggcgcacg tactaccgaa
     4621 agtcagatcc accgtgaaat ggcttcggca ctctcctgtc cggtaggttt taaaaatggt
     4681 accgatggca atacgcggat tgctgtggat gctatccgcg cagcccgcgc cagccatatg
     4741 ttcctctcgc cagacaaaaa tggtcagatg accatctatc agaccagcgg caacccgtat
     4801 ggccacatta ttatgcgtgg cggcaaaaaa ccgaattatc atgccgatga tatcgccgca
     4861 gcctgcgata cgctgcacga gtttgattta cctgaacatc tggtggtgga tttcagccac
     4921 ggtaactgcc agaagcagca ccgtcgccag ttagaagttt gtgaggatat ttgtcagcaa
     4981 atccgcaatg gctctacggc gattgctgga attatggcgg aaagtttcct gctcgaagga
     5041 acgcaaaaaa tcgtcggcag tcagccgctc acttacggtc aatccattac cgacccgtgt
     5101 ctgggctggg aagataccga acgcctggtc gaaaaactcg cctctgcggt agatacccgc
     5161 ttctgaatgc gtgcccattc ctgacggaat gggcatttct gcgcaacttg ttgtcttctc
     5221 aacaaattac tgcttgctct ggcctgccat aatattga
//
LOCUS       DAEEKQ010000225         5246 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.227, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000225 DAEEKQ010000000
VERSION     DAEEKQ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5246
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.227"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..323
                     /gene="gatA"
                     /locus_tag="KLK57_003889"
     CDS             <1..323
                     /gene="gatA"
                     /locus_tag="KLK57_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HBE0894137.1"
                     /translation="AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQAD
                     DDNDVEVSLVIALIVENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVL
                     DSDE"
     gene            354..638
                     /gene="gatB"
                     /locus_tag="KLK57_003890"
     CDS             354..638
                     /gene="gatB"
                     /locus_tag="KLK57_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HBE0894138.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQNHSIPVELIQCRVNEI
                     ETYMDGVHLICTTAKVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            642..1997
                     /locus_tag="KLK57_003891"
     CDS             642..1997
                     /locus_tag="KLK57_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HBE0894139.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSTQNIPGFIIIGAIYLTGIFMTWRRARGFIKQEKTVLA
                     E"
     gene            2045..3085
                     /gene="gatD"
                     /locus_tag="KLK57_003892"
     CDS             2045..3085
                     /gene="gatD"
                     /locus_tag="KLK57_003892"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HBE0894140.1"
                     /translation="MKSMVNDTDGIVRVAESVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTYPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            3191..3964
                     /locus_tag="KLK57_003893"
     CDS             3191..3964
                     /locus_tag="KLK57_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE0894141.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(4046..4945)
                     /gene="yegS"
                     /locus_tag="KLK57_003894"
     CDS             complement(4046..4945)
                     /gene="yegS"
                     /locus_tag="KLK57_003894"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HBE0894142.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDGIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCLLLR"
BASE COUNT         1318 a         1154 c         1345 g         1429 t
ORIGIN      
        1 aagcagaatt ccctaccggg ataatgcttg agcagcacgc cattgcaata ccgcattgtg
       61 aggcgattca tgctaagtcg tcagccattt atctgttaag gccaacaaat aaagttcatt
      121 ttcagcaagc ggatgatgat aacgacgtgg aggtatcgtt ggttattgcg ttgattgtgg
      181 agaatccgca gcagcaattg aaacttttac gctgtttatt tggcaagtta caacagcccg
      241 atatcgtcga gacactaatc actcttcctg aaacccagtt aaaggaatac ttcacaaagt
      301 atgttttaga ttcagacgaa taaatccctc tgtaacaata aaaaggacta tttatgaaac
      361 gcaagattat tgtcgcttgc ggaggcgcgg ttgcgacctc tacgatggcg gcggaagaaa
      421 ttaaagagtt gtgtcagaat catagtattc ctgttgaatt aatccagtgt cgggttaatg
      481 aaatagaaac ctatatggat ggtgtgcatt tgatatgcac cactgccaaa gtggatcgta
      541 gttttggcga tattccgtta gttcacggca tgccttttat ttctggtgtc ggtatcgaag
      601 cattacaaaa taaaattctg actatcttac aggggtgacc tatgttttca gaagtcatgc
      661 gttatattct cgacctcggc cctacggtga tgcttccgat tgtcatcatt attttttcta
      721 aaatattagg catgaaggca ggcgattgct ttaaagcggg tctgcatatc gggattggct
      781 ttgttggcat tggccttgtg attggcttaa tgctggattc cattggtccg gcggcgaaag
      841 cgatggcgga aaatttcgac ctgaatctgc atgtggtcga tgtcggctgg ccgggctctt
      901 caccaatgac ctgggcgtcg caaattgcgc tggtggcgat tccgattgcg attctggtta
      961 acgtggcgat gttactgacc cgtatgacgc gggtggtaaa tgttgatatc tggaatatct
     1021 ggcatatgac cttcaccggc gcgttgctgc atctggcaac cggttcatgg atgataggga
     1081 tggcaggtgt ggtaattcac gcggcgtttg tttataagct cggcgactgg tttgcccgcg
     1141 atacccgaaa tttctttgag ctggaaggta ttgctattcc gcacggtacg tcggcgtata
     1201 tggggccgat tgcggtgctg gtcgatgcta tcatcgagaa aatcccaggc gttaaccgaa
     1261 ttaaatttag cgccgacgat attcagcgca aatttggtcc atttggcgag cctgtcaccg
     1321 tgggttttgt gatggggctg attatcggca tcctcgccgg ttacgatgtc aaaggtgtat
     1381 tgcagctggc ggtaaaaacg gcggcagtta tgctgctgat gccacgggtg attaaaccca
     1441 tcatggatgg tttaacgccc atcgctaagc aggcccgtag tcgtttacag gcgaagttcg
     1501 gcggtcagga gttcctgatt ggcctggatc ccgcattact gctggggcat acggcggtgg
     1561 tatcggcaag cctgattttt atcccgctca ccattttaat tgctgtttgt gttccgggta
     1621 atcaggtact gccgtttggc gatcttgcca ccatcggctt cttcgtggcg atggcggtcg
     1681 cggtgcatcg tggaaatctg ttccgcacct taatctcggg tgtcatcatt atgagcatca
     1741 ccctgtggat cgcgacgcaa actattggtt tgcacaccca actggcggct aatgctgggg
     1801 cgttaaaagc cgggggtatg gtggcttcaa tggatcaggg cggttctccc attacctggt
     1861 tactgattca ggttttctcc acgcaaaata ttcccggttt cattattatc ggcgcaattt
     1921 atcttaccgg tattttcatg acctggcgta gagcgcgtgg ttttattaaa caagagaaaa
     1981 ccgttctcgc agaataattt ttacctgagg gggaggttat cccccttaac aatcaggagt
     2041 ttttatgaaa tcaatggtga atgatactga tggtatcgtg cgcgttgcag aaagcgtcat
     2101 tcctgaaatt aaacatcagg atgaggtgcg ggtaaaaatt gccagctcgg gcttatgtgg
     2161 ttccgattta cccaggatat ttaaaaatgg tgcacattat tatccaataa cgttaggcca
     2221 tgaatttagc ggctatattg atgcggtggg atccggtgtt gatgatttac accctggcga
     2281 tgcggttgcc tgtgtgccgt tattaccctg ttttacttat ccagagtgtt tgaaagggtt
     2341 ttattcccag tgcgcaaaat atgattttat tggctcgcgg cgtgatggtg gatttgctga
     2401 atatattgtc gttaagcgaa aaaatgtctt tgctctaccc acggatatgc ctattgagga
     2461 tggggctttt attgagccga ttaccgttgg cctgcatgct tttcatttag cgcaaggttg
     2521 tgagaataaa aacgttatta ttattggtgc cggaaccatt ggcctgctgg ctattcagtg
     2581 cgctgtcgcg ctgggagcaa agagtgtgac ggcgatcgac attagctcag aaaaactggc
     2641 attggcaaaa tctttcggtg cgatgcaaac atttaacagt agcgaaatga gcgcgccgca
     2701 aatgcagagc gttttacgcg aactgcgctt taatcagctt atcctcgaga cggctggcgt
     2761 accgcaaact gtcgaactgg cggtagagat tgccggtcct catgcccaac tggcgctggt
     2821 gggcacgttg catcaggatc tgcatttaac atcggcaacg tttggcaaaa tattgcgtaa
     2881 agagctgacg gttatcggca gttggatgaa ctattccagc ccttggccgg ggcaggagtg
     2941 ggaaacggcg agccggttgc tgacagaacg taagttaagc ctggagccat taatcgctca
     3001 ccgtggaagc tttgaaagct tcacccaggc ggtgcgtgac atcgctcgta atgccatgcc
     3061 gggcaaagtg ttgctcattc catgaaaccg cgggccagcg tgatgctggc ccggtattgt
     3121 gcaaaacaga ttattcacca atggtccccc ttcgtttaca ctagccacaa ttgaatatgg
     3181 gtaaatgacg atgaattcat tcgagcgaag gaataagatc atccaattag tgaatgaaca
     3241 gggaaccgtg cttgttcagg atctggcggg agtatttgct gcctcggaag cgacaatccg
     3301 tgccgatttg cgctttctcg aacaaaaagg cgtggttacg cgctttcatg gcggtgcggc
     3361 gaaaataatg tccggtaata gtgaaaccga gacccaggaa gtcgggttta aagagcgatt
     3421 tcagctcgcc agcgcgccaa aaaacagaat agcgcaggcg gcagtcaaaa tgatccatga
     3481 agggatgacc gttattctcg acagcggaag tacgacaatg cttatcgccg aaggattaat
     3541 gaccgctaaa aatatcacgg tgattaccaa cagtctcccg gcagcgtttg ccctttccga
     3601 aaataaagac attactctgg tcgtctgtgg tggcacggtc cgccataaaa cgcgctcgat
     3661 gcatggttcc attgccgagc gttcattgca ggatattaat gccgatttaa tgtttgtcgg
     3721 tgctgatggt attgatgcgg ttaatggtat tacgaccttt aatgaaggtt attcgattag
     3781 cggtgcgatg gtcaccgccg ctaataaagt gattgccgtt ctcgattcat cgaaatttaa
     3841 ccgtcgcggt tttaatcagg tactgccaat cgaaaaaatt gacattatta ttaccgatga
     3901 tgctgtatca gaggtggata aactggcatt gcagaagaca cgggtaaaat taattacggt
     3961 gtagtattgt tctgtatgcc ggataaggta tttacaccgc atccggcatt tcggattctc
     4021 cctggttcaa gagagaatag tttgattaac gcaataatag acaatctggt ggtaatcgac
     4081 aacgcaacgc cgccggaagt atttcaatat gaaagttttg tccgctcaac ggttcgccat
     4141 caagattaaa agtgatttcg tgtggggctt gtatatcaaa ccacgacgaa gcgccttcga
     4201 taatattcgg gttatcttcg tcagatttta aggttgatac gagagccgga agaatttcat
     4261 cgccggtaaa aatgcgcagt tgcagcaagc catcgttaat taacgcgttc ggacacaatt
     4321 gctgaccgcc accggcctga cgcccgttac caataccaat gaccagggcg tcaccttgcc
     4381 agtgaaagtt ttcaccgcgg atttcacaac ggtccggttg cagagtatcc atgcgcatta
     4441 agccatgaat gatgtaagag acgccaccca gcgcggcttt taatttttcc ggcgtttctg
     4501 tggtaatacg cgtcccaaat ccgcctgtcg ccatattaat aaaacaggtt tgtttgttga
     4561 cctgcgccat atctatcgca atggcgttac cggcaattgc cagtttcagc gccttatcca
     4621 gtgcctcagg aatccctaca ctggtggcaa aatcattggc ggttcctaat ggcaaaattc
     4681 ccagcgcggg tatgccatcc ccctcacact gaatcaacgc cgtagaaact tcattaatgg
     4741 tgccatcgcc accaccggca atcaccgttg cgacgcccaa cttccgggcc tcctctacat
     4801 atcgtgcggc atcgcctttc tcccaggtga cccgcacatg gatcgtcatt ccttcctcac
     4861 gcaacagcat aattgcttcg cgcaagggta gattgtcagt acttttgcca ttaagaatca
     4921 gtaagctggc gggaaattct gccatgatcg tatgtgcctt tatgattggt ctgtagatag
     4981 tgtagagcag aaaacaaaag gtggagtcag aacaggatga aagtcggagg ataaaaagtc
     5041 agttcaagta tggaggattc agtggttacc accaggagct tgcaatgggg acagcgaata
     5101 ctaacggctg ccaccgataa atttcaaaaa agagcatata cctaatattc aactaaacag
     5161 tggcatcttc aatataatat attaaagccc ccatggagtt accctgaagg gcctcaatgt
     5221 ccgtaattcc tacttatgta ggaaat
//
LOCUS       DAEEKQ010000226         5226 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.228, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000226 DAEEKQ010000000
VERSION     DAEEKQ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.228"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..2777)
                     /locus_tag="KLK57_003895"
     CDS             complement(<1..2777)
                     /locus_tag="KLK57_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HBE0894143.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNGLRYMI
                     YPHAQPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETLYQVSLPTTQKQNLQQVMAIFSEWSNAATFEILEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSLAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQVSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLP"
     gene            complement(2834..5206)
                     /locus_tag="KLK57_003896"
     CDS             complement(2834..5206)
                     /locus_tag="KLK57_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HBE0894144.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVTGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTCGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMAEHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQDLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
BASE COUNT         1298 a         1220 c         1195 g         1513 t
ORIGIN      
        1 ggcaataata ctccagtata agtatttacc ggatgagaaa gatattgttt aacggcagtg
       61 ttaacattct ctaccgtcat ttgtttcaac aattgctcct gctcagtcca tgctgcagga
      121 tcgtcatatt gaataagact atttacaata gtgttcgcta attgttgaac gctacgctgt
      181 tggatatcga ggctgcgctg aacgttttgc tggtattcat tcagttcttg ctcactgatc
      241 cctttagcca gacgcttaac catcacttca ttcgctaacg ttaacagttc atcatgtcgt
      301 tctggttgac aagtaaaagc cagcaaatga ctgatatctt tggcctgagg atcaaccgag
      361 aggcgagaag aaacgctgta tgctccagat gcctgttcac gaatattaac gcgtagatct
      421 tttgccagtg cgacgttaaa agcatcgagc gccatacgcg tcgccagatt aacaggtgtc
      481 cgggaatcat aacgcttcca ctgtgaaacc tgtgccacag gttcattttg ttcttttaca
      541 gtaaccgatg cgttgtccgt cgcgcgagtt aatggtttac ctgcggctaa tggcgaatca
      601 gagtgtttga ttgatcctaa gtaacgcgta attaacgcca cgagtttgtc ttctgagaca
      661 ttaccgacaa tgacaaacgt gatatccgct ggagatgaaa acaactggcg atcggcagcc
      721 agcgcatctg cggcagtaaa ctgtacaatc tgattttctt gcagtaattt cgtgcggtca
      781 tcagcatagc gcgtctcata catctgctgg gcgaatttct ccgctggacg ctggtcgagc
      841 gttttcaacg cctgaatttg agcattttgt agcgatgccc aaatattgtc gttaatcgtg
      901 ctgtgggtga ttcgctggtt aatcaactga aaaccaggtt cagggttatt agtccgcgcg
      961 ctaacggaga gcaacgtatt catgccactg actttactgc tcatggttac cgaattttcc
     1021 gcactccagc gtttcaggct ggaagaggag agctcaccaa cgccgcttcc gcttactgct
     1081 ttatttgcca gcgcgataag tgatttttgc tgcgcaggga aacttaaatc gcctttatta
     1141 gatacggcag taatttgtag cttttgctct tcacccgcgg attttgccag aataaccctg
     1201 gcaccattgg aaagtgttaa tgatgtcaga ttctcagcca gcgtttcttt gctgctaatt
     1261 tccgcctgtg ggtcagcgtc tactgttaac gataaatttc tgcctgggaa gatgtaagcc
     1321 gccagctttt tgttggcgta ctctttttcc agcgccagga tagccgcaag agacaatgct
     1381 tttttggcgg caagctcatt gtttaccatt tgctcccaaa atgcgtcctg gttctttctt
     1441 aactgctgcc atttttccgc cagactttgc acggtaattt gctgccacag acgtttcgaa
     1501 agttgatatg tctcttccgg cgacaagaac ggcgtattat ttaatgagct ggatgccagg
     1561 cgactggtca gcatacgtaa atcacgttca gcttgctgat caaccgcatt tttcagccag
     1621 gtgaggcggg tagatttgac atcatcgagt tcttcagcag aaaaaccatg ctgatcaatg
     1681 gttgccaact ctgccattaa tgcatttgca gcatcctgca tattatcgtc tcgtgcattt
     1741 acacggaaaa acagcgactg ataatcgggt gcaattttaa cgctgcgcgc agtgccgcca
     1801 gaaatagtct tcaactcgcc cgactgtatg cgttcctgca gacgttgatt gaacagctga
     1861 actaacatgc tccattcagc ttgttcgaca aagctttgct catcgttcac ttgtaccatt
     1921 ggcaggcgat aatagagtgc gatgccgttc actcggtttt ctttatcatt aatgatatta
     1981 aagcgcaggt ggttttcggc ttttgtcggc cagacgcgat tttcagctgc tttgttagcc
     2041 ggaagcttac ttaaattatc ctttatcagc gccagcgctt ctttactgtc gatatcgccg
     2101 accacgataa aggtcatatt atttggttga taccagcgtt gataaaattg gcgcaattgt
     2161 gccggtgtga ccgtggcgac agtatccatc aggccgatag gttcacggtc taaattacgg
     2221 gtatttgcca gcaggaaagg gcggcgcgcc tgagaggtgc gccatttcgc atcctgatgg
     2281 gcacgccatt cctcagtaat tacgccgcgt tcagcgtcta cttcgagtat ttcaaaggtt
     2341 gcggcattac tccattcact gaagattgcc atcacttgtt gcagattttg tttctgcgta
     2401 gtcggcagac tcacctgata cagcgtttcg tcatagctgg tataggcatt aacatcgcga
     2461 ccgaaacgca ggcccattga ctcaaatgtt tcgatgactt tattacccgg ccatgttttt
     2521 gtgccgttaa acatcatatg ttctacaaaa tgagccacgc cgcgctcatt gtcttcttcc
     2581 tgcaatgaac cggtatgaat ttgcagccat aaatttacct gatcctttgg ttgagcatgc
     2641 ggataaatca tatatcgcaa gccattgtcc agttgcccgg taattaactt ttcatcctgc
     2701 ggtaaggcgg cggcaatcag ccgcccgggg agcaacagag ttgccactaa cgtcagtaag
     2761 aaacagaggt ttctcataat tatctccatg cgaaaaccgg gcgaatttac ccggttaagt
     2821 aaaatccgaa ctattaaaat ttcatgctga catcaagcca gaaagtacgg ccactggcgt
     2881 aggtcgccac accggtgttc gttgtatcaa cagcggtttt gctatccagt acattgagaa
     2941 tatcggcact gatcgtcagg ttttgttgtt tcagaaattg tggcgtccag gacaagcggg
     3001 tgtcccaggt caggctgcta tccagttttt cgtcaacata ctgcttgtaa tctgaatatt
     3061 cgctgatgta ttgcgcattt gtcttaccaa ggataatgcg agctttacgc gcttcttgcc
     3121 aggccagcgt attcgcccac actaacccgc tcggttgatg cgtgaaatcc atgtttaagg
     3181 agatctttaa tgggttatta aaatctgcca ctggaacact atcgtaagag accagattac
     3241 cgttataaac cacctggtta tcaccggtat tgctctcctc ataaccattg tttaacgaca
     3301 agttgccctt gctcttgata tagctaaaga caatttgtgg gttaatatct acttggcgga
     3361 tatgcagggg ttcggccagt tcaaaactga ggctgaacga atgcgttttg gttttgccat
     3421 cgttgttata ttcagtaatg gtggttttag tcgcgctgtc ggtacgactg cttttgctga
     3481 tttgatcatg cgcttcacgg taaacatagt ttgcacgcgc aataacgttc ttaccgattt
     3541 tctgctgcaa tcccattgcc agttcatcgt tataaggcgt tttcaaatcc tgataacgcg
     3601 tcagagtttt attacctgat accgattccg tccagctatt gcggatatca cgtaatccca
     3661 tatcaagaat attcccgcca tagtaacggt tataaccggc ggtaatcatt gaggtttgat
     3721 cagcaaaaat atcccattcc gtcataaagc gcggggagat attgtggttt gacagatagt
     3781 tgtcatagtc ataacgcaca ccgggcatta acgacacatt ccgccagcta atgtgctccg
     3841 ccatatacag cgtgtagttg tcaattccca ggctgccttt acctttatgg taaatggtat
     3901 ggttagtttt ctttccggca gcattaatca cataggattc agactggtta tggcgttcag
     3961 tccatgcatc ggaatagatg tattccgcgc cgaagtaggg ttgatgcgaa acatcaccca
     4021 cggcgaattt ttgccagtcc aggcgtgttt tgaaggtgta attatctaca gcctgggaaa
     4081 tgtgtcctaa tccgccacag gtacaacgcc ctgtaatatc accatatgta catgaaagtt
     4141 cggtgtacca gatgtcatga tcgtgacggg tataatcact aatatgatcc caaccaacgg
     4201 tggtacgtag tttggcccat gcgagttgcg tatccatatc ccatgccaga ccataggatt
     4261 tattacccat ttcgcgatca gactgcggga aggtgctggt attataatca cggctggagc
     4321 cggtatattt taaggttaaa tcgtgggtaa agcggtcgct ggcaaaccag gtaaatttgc
     4381 tcaatgcagt atcgataacg tttttatact gtgcccgacc ggtgacaatg ccgtcattcg
     4441 aaacataatc cgcgcgggtg atatcagact ggcggcgcga taaaccggcg gtaacgccga
     4501 agttatcagc gagttcctga ttaaacgaca aggtataaaa gttcttttta aaatctgggg
     4561 agtaataagt acttcctgaa gagccttgat taaatgcgct cttgttattc tcatcgatat
     4621 gcgatgttaa ccagtccgaa cgcgtagtgc gataacccaa tttcaccttg ctatcatcag
     4681 cgttgaagcg tttgatcttt gcatcaatta ccccgccatt gaagcgacca aattcaaccg
     4741 gcacaaaact gtcataaagc gtcacattgt ccagtaagct gacatcaaga taatacccct
     4801 gtgacatccc gctaatattg gttgcactac tggcatcgga ctcattcgct gggttcaggt
     4861 tattagttgc actaataccg tcaatcaaat aggcattctg gtagggcgac gcaccgtgaa
     4921 tagagatttt ctcagggcga atatctccct ggttcaacga ggtactttgc gttgaatcca
     4981 tgcgtaccgc agggttggtt ctcagcagat cgctgatatt gccattcccg gtcggtaaac
     5041 gctcgatgct ttcactggtg taatgcgtgt tgccgtttac gggtacaggg acaggagcat
     5101 agaccgtcat ctcttcaaaa aaatgcatgt acatgttttt gtcatcgacg gcctgcgcca
     5161 catcagcgcc acataaaatg acgccaggaa taagaactcg cttcatttat ttatatctcc
     5221 gtacag
//
LOCUS       DAEEKQ010000227         5219 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.229, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000227 DAEEKQ010000000
VERSION     DAEEKQ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.229"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(291..575)
                     /locus_tag="KLK57_003897"
     CDS             complement(291..575)
                     /locus_tag="KLK57_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894145.1"
                     /translation="MISQNVWAPPFFFGPWVELENHTGDIAYTVYFDTLSNVPGPFIA
                     FIWYAPNGPRTVNVIGPASYQISCKGAGVDKIKFKSVSGTGQIIHVYTTL"
     gene            complement(593..790)
                     /locus_tag="KLK57_003898"
     CDS             complement(593..790)
                     /locus_tag="KLK57_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001335786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894146.1"
                     /translation="MKEEKVLLHRFLFVVRNKNGCELSCSADLMGTRDDVYKYFSDSV
                     SGLDVELIDVSCESEWEEHSH"
     gene            1002..1298
                     /locus_tag="KLK57_003899"
     CDS             1002..1298
                     /locus_tag="KLK57_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001679964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894147.1"
                     /translation="MSKTKSGRHRLSKTDKRLLAALIAAGYEERTARDLIQKHVYTLT
                     QADLRHLVSEISNGVGQSQAYDAIYQARRIRLARKLLNGKKPEEGLNAWKGDLP"
     gene            complement(1843..2076)
                     /locus_tag="KLK57_003900"
     CDS             complement(1843..2076)
                     /locus_tag="KLK57_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HBE0894148.1"
                     /translation="MTELERHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVQHLSEQTERLSQLYSENRR"
     gene            complement(2083..2613)
                     /locus_tag="KLK57_003901"
     CDS             complement(2083..2613)
                     /locus_tag="KLK57_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HBE0894149.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTETILNDEFRKLEKSVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNENLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            complement(2591..3313)
                     /locus_tag="KLK57_003902"
                     /pseudo
     CDS             complement(2591..3313)
                     /locus_tag="KLK57_003902"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            complement(3291..3614)
                     /locus_tag="KLK57_003903"
     CDS             complement(3291..3614)
                     /locus_tag="KLK57_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HBE0894150.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGKLPT
                     LAPPLLRQLAAIGNNLNQTARKVNSGQWSSGDRVQVVAALMAIERELRSLRQVVREHG
                     ARDDS"
     gene            3679..3870
                     /locus_tag="KLK57_003904"
     CDS             3679..3870
                     /locus_tag="KLK57_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBE0894151.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH
                     ADELYRSCLARFGDDGENL"
BASE COUNT         1377 a         1422 c         1110 g         1310 t
ORIGIN      
        1 aaaaaaacaa aggcgtcata atagaagtaa acacagatag tttatgtaag aaaaaactaa
       61 acgaaataac tagtaaaaat atagggaaac ccctgatttc atattttgat ggaaaagtaa
      121 tatcccaacc tgcaatcatt cattctgcac taaatccaac ccattattat caacctgtga
      181 acgatgaaac aacagtgatg gaaatcataa aagcatattc aaaataatat taatagatat
      241 aatcctgctt aaggtatagc ttaggcagga ttatatctat tcagctctta ttataaggta
      301 gtgtatacat gaataatctg ccctgtccca ctgacacttt taaatttaat tttatcaaca
      361 ccagcgccct tgcaagagat ctgataagat gctggaccaa taacgttaac tgttcttggc
      421 ccattagggg cataccagat aaaagcaata aatggaccgg gtacattaga caatgtatca
      481 aaatagacag tgtacgcgat atcaccagta tgattttcaa gttcaaccca aggaccaaaa
      541 aagaaaggag gagcccaaac attttgagaa atcatataat ctcctgatta gttcaatgcg
      601 aatgttcttc ccattcactc tcgcaagaaa catctataag ctcaacatct agcccactaa
      661 cagaatcaga gaaatactta taaacatcat cccttgttcc cataaggtct gccgaacatg
      721 atagttcaca accattctta ttcctaacta caaataagaa acggtgaagc aatacttttt
      781 cttctttcat tttatccctc cctttattta cacagaatat actgtatata taaatactag
      841 ttttatatac agtataaatt acgccgcatt atttttcctg tcaatgatat gaattttcat
      901 ttttgtgatc tgaatcaccc ttataaaaat caggaaggga agattcgcag cagaaaaaca
      961 gcaccggata acatcaagag aaaaaacaga gaggagataa cgtgagcaaa acaaaatctg
     1021 gtcgccaccg actgagcaaa acagacaaac gcctgctggc tgcacttatc gctgccggat
     1081 acgaagagcg gacagcccgt gacctcatcc agaaacacgt ttacacactg acacaggccg
     1141 acctgcgcca tctggtcagt gaaatcagta acggtgtggg acagtcacag gcctacgatg
     1201 cgatttacca ggcaagacgc attcgtctcg cccgtaaact cctgaacgga aaaaaaccgg
     1261 aagaagggct gaacgcctgg aagggtgatt taccataatc acttattgta cgcaccgctg
     1321 aaacgcgttc ggcgcgatca cggcagcaga caggtaaaaa tggcaacaaa ccacccgaaa
     1381 aactgccgcg atcgtacccg gtaaatttta accacatgca tagctatgca gctatgtgaa
     1441 tcatgcagga attgccagcc agagacagct gaaacggatt tttttcatgc tttcggaagc
     1501 gaagaagacg gggaccggag cgggaaaaca gaagttcagg ggaatgagcg cgatctggca
     1561 atagaggcaa aacacagcaa caaaagacac accagaatcg cgcccgtatg cgttttaacg
     1621 cgttcctgtg ttttcagcaa ggtctgactg aagcgcgttc ccggtgcccc cgaaccaacc
     1681 attatcccaa aaaatgcacc ggaaacgcgc tgacaacatt ttagcctgtc tctgattacc
     1741 atcccagcga agggccatcc agtgtccgtt cctgtatttc cagctggcgc tcacgctcca
     1801 gggataacgc atgttcgcgt tcctgtatca gccgttcgtc ccttatctcc tgttctcgct
     1861 gtataactgg ctcaagcgtt ctgtctgctc gctcaagtgc tgcacctgct gactcaactg
     1921 catgacccgc tcgttcagca tcgcgttgtc ccgttgcgta agcgaaaaca ttttctgcaa
     1981 ttccacgaag gcgctctccc attcgttcag ccgctgcata tagtcctgtt gcagttgctc
     2041 taatgcgttc agcaaatgtc tttccagctc cgtcatgctc tgttactcct gtaccagcct
     2101 tacggcattc tgtttcccgt tttccatcgt ccagttcgtt tcggctttca tcccctcggg
     2161 caacaccaga aaacgtccgt tgctcccctc gtgataacgc acgccccatg tcttcgcgtt
     2221 cagcttctcc agattctcgt tctgcttcct gatttcttcc aggttcgcct gtatcatccc
     2281 gccaagatac cagagcgtcc cgccactcac ggtaaacagg aaaatgacca tccccagtaa
     2341 catcatgccc gtattccctg ccagctttaa cacgtccttc ctgtgctgca tcatcgcctc
     2401 tttcacccct tcccggtgtt ttttcagtga ttcctctgtc gaagctgtga acagggctat
     2461 agcgtctttg attttcgtct cgtttgatgt cacagccttg cttacagatt tttcgagctt
     2521 cctgaactcg tcgttcagta ttgtctctgt agattcggct ctctctttca gctttttctc
     2581 gaactccgcg cccgtctgta aaagattgct cataaaatgc tcctttcagc ctgatattct
     2641 tcccaccgtt cgggtctgca atgctgatac tgcttcgcgt caccctgacc acttcaagcc
     2701 ctgcctctgt gagcgcctga atcacatcct gacggccttt tatctccccg acatgataaa
     2761 gagcgtctat cccgcgtgtg acgctctctg caagcgcctg tttcgtttcc ggcaggttat
     2821 cagggagagt cagtatccgg cggttctccg gggcgttggg gtcatgcagc ccgtaatggt
     2881 gattaaccag cgtctgccag gcatcaattc tcggcctgtc tgcccggtcg tagtacggct
     2941 ggaggcgttt tccgctctgt agctccatgt tcggaatgac aaaattcagc tcaagccgtc
     3001 ccttgtcctg gtgctccacc cacaggatgc tgtactgatt tttttcgaga ccgggcatca
     3061 gtacacgctc aaagctcgcc atcacctttt cacgtcctcc cggcggcagc tccttctccg
     3121 cgaacgacag aacacctgac gtgtatttct tcgcaaatgg cgtggcatcg atgagttccc
     3181 ggacttcttc cggattaccc tgaagcaccg ttgccccttc ccggttacgc tcccttccca
     3241 gcaggtaatc aaccggacca ctgccaccgc cttttcccct ggcatgaaac ttaactatca
     3301 tcccgcgctc catgttccct gacgacctgc cgtaagctgc gcaactctct ctcgatggcc
     3361 atcagtgcgg ccaccacctg aacccggtca ccggaagacc actgcccgct gttcaccttc
     3421 ctcgccgtct gattcaggtt attcccgatg gcagccagct ggcgcagtaa cggcggtgcc
     3481 agtgtcggca gcttcccgga acgggcaacc ggctcaccca gacagacccg ccgcatccat
     3541 accgccagct gtttaccctc acagcgttca agtaaccggg catgttcatc atcagtaacc
     3601 cgtattgtga gcatcctctc gcgtttcatc ggtatcatta tccccatgaa cagaaatccc
     3661 ccttacacgg aggcatcagt gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt
     3721 ataagaagcc agacattaac gctgctggag aagctcaacg aactggacgc ggatgaacag
     3781 gcagatatct gtgaatcgct tcacgaccac gccgatgagc tttaccgcag ttgcctcgct
     3841 cgcttcgggg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     3901 tgtctgtgag cggatgccgg gagctgacaa gcccgtcagg gcacgtcagc gggttttagc
     3961 gggtgtcggg gcgcagccct gacccagtca cgtagcgata gcggagtgta tactggctta
     4021 accatgcggc atcagtgcgg atggtatgaa aagtgcgcca taccgggtgt gaaatgccgc
     4081 acagatgcgt aaggagaaaa tgcacgtcca gccgcttttc cgcttcctcg ctcactgact
     4141 cgctacgctc ggtcgttcga ctgcggcgag cggtatctac tcactcaaaa acgaaatgct
     4201 gttatccaca gaatcagggg ataacaccgg aaaaaacatg tgagcaaaag accaggaaca
     4261 ggaagaaggc cgcgccgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     4321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg acttaaagat accaggcgtt
     4381 tccccccgga agctccctcg tgcgctctcc tgttccgacc ttaccgttta ccggatacct
     4441 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4501 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4561 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4621 aacgccactg gcagcagcca ttggtaacta aaaagtggat ttagatacgc agaactcttg
     4681 aagttgaagc cttatagcgg ctacactgaa aggacatctt ttggtatctg tgctccactt
     4741 aagccagcta cccggttagg taatctgcca ggattagcta accttcgaaa aaccacctgc
     4801 tagggtggtt ttttcgttta caggcagcag attacgcgca gaaaaaaagg atctcaagaa
     4861 gatcctttga tcttttttct ctaaactgca atcccatcag ttcaggagag aaagatagta
     4921 taattgccct ctcttatatg gaaccaccat tcaatcacat catgggaata atataagtga
     4981 aaacgttcgc attgtttatt tttacgtgta tatgtattta tagattggaa ttttaacaaa
     5041 ataaaatgga gttaatatgt tcaaatcatc agtaaagtca ataacatcga gtactatact
     5101 gaccttactt ataggatgtt caagtactga aacaaacaaa aacattgttt acaaagttgg
     5161 tggtgacagc atcattctga ataaatattg catacttaaa atgtaccctt cccaatcag
//
LOCUS       DAEEKQ010000228         5180 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.231, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000228 DAEEKQ010000000
VERSION     DAEEKQ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5180
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.231"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            81..1253
                     /gene="istA"
                     /locus_tag="KLK57_003905"
     CDS             81..1253
                     /gene="istA"
                     /locus_tag="KLK57_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family transposase"
                     /protein_id="HBE0894152.1"
                     /translation="MLSREDFYMIKQMRQQGAYIVDIATQIGCSERTVRRYLKYPEPP
                     ARKTRHKMVKLKPFMDYIDMRLAENVWNSEVIFAEIKAMGYTGGRSMLRYYIQPKRKM
                     RPSKRTVRFETQPGYQLQHDWGEVEVEVAGQRCKVNFAVNTLGFSRSFHVFAAPKQDA
                     EHTYESLVRAFRYFGGCVKTVLVDNQKAAVLKNNNGKVVFNSGFLLLADHYNFLPRAC
                     RPRRARTKGKVERMVKYLKENFFVRYRRFDSFTHVNQQLEQWIADVADKRELRQFKET
                     PEQRFALEQEHLQPLPDTDFDTSYFDIRHVSWDSYIEVGGNRYSVPEALCGQPVSIRI
                     SLDDELRIYSNEKLVASHRLCSASSGWQTVPEHHAPLWQQVSQVEHRPLSAYEELL"
     gene            1253..2050
                     /gene="istB"
                     /locus_tag="KLK57_003906"
     CDS             1253..2050
                     /gene="istB"
                     /locus_tag="KLK57_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS21 family helper ATPase
                     IstB"
                     /protein_id="HBE0894153.1"
                     /translation="MHELEVLLSRLKMEHLSYHVESLLEQAAKKELNYREFLCMALQQ
                     EWNGRHQRGMESRLKQARLPWVKTLEQFDFTFQPGIDRKVVRELAGLAFVERSENVIL
                     LGPPGVGKTHLAIALGVKAVDAGHRVLFMPLDRLIATLMKAKQENRLERQLQQLSYAR
                     VLILDEIGYLPMNREEASLFFRLLNRRYEKASIILTSNKGFADWGEMFGDHVLATAIL
                     DRLLHHSTTLNIKGESYRLKEKRKAGVLTKNTTPISDDEMVESGQHQ"
     gene            2110..3211
                     /locus_tag="KLK57_003907"
                     /pseudo
     CDS             2110..3211
                     /locus_tag="KLK57_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3280..3714
                     /gene="psiB"
                     /locus_tag="KLK57_003908"
     CDS             3280..3714
                     /gene="psiB"
                     /locus_tag="KLK57_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugation system SOS inhibitor PsiB"
                     /protein_id="HBE0894154.1"
                     /translation="MKTELTLNVLQTMSAQEYEDIRAAGSDERRELTHAVMRELDAPD
                     NWTMNGEYGSEFGGFFPVQVRFTPAHERFHLALCSPGDVSQVWVLVLVNAGGEPFAVV
                     QVQRRFAPEAVSHSLALAASLDAQGYSVSDIIHILMAEGGQA"
     gene            3711..4430
                     /locus_tag="KLK57_003909"
     CDS             3711..4430
                     /locus_tag="KLK57_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid SOS inhibition protein A"
                     /protein_id="HBE0894155.1"
                     /translation="MSARSQALVPLSAEQQAAWRAVAETEKRRHQGNTLAEYPYAGAF
                     FRCLNGSRRISLSDLRFFMPSLTAEELRGNRSQWLYAVDVLIETQGEVCLLPLPGDAA
                     ERLFPSVRFRVRERSRHKSALVMQKYSRQQAREAEQKARAYQALVAQAEIELAFHSPE
                     TVGSWHARWSDRVAEHDLETLFWQWGERFPSLAGMERWQWQDMPFWQVIAEASLAARE
                     AGHAVREMERWMVPNKLREAA"
     gene            4710..4868
                     /locus_tag="KLK57_003910"
     CDS             4710..4868
                     /locus_tag="KLK57_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBE0894156.1"
                     /translation="MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAA
                     FMAYESGK"
BASE COUNT         1224 a         1319 c         1561 g         1076 t
ORIGIN      
        1 acggtaattc gccatttgga ttgtccgctc cacccaacat gttgtttcct taaggttctc
       61 acaccagaaa ggacatcaac atgctgagca gagaggactt ttacatgata aagcaaatgc
      121 gccagcaggg cgcgtacatc gtcgatattg cgactcagat tggttgctct gaacggacgg
      181 tcagacgcta cctcaaatac cctgaaccgc cagccagaaa gacccgccac aaaatggtta
      241 agctgaaacc gtttatggat tacatcgaca tgcgcctggc agagaatgtc tggaatagcg
      301 aggttatctt cgcggagatt aaggcaatgg gttatacggg cggacgttcc atgctgcgtt
      361 actacatcca gcccaaacgt aaaatgcgtc cgtcaaaaag aacagttcgc ttcgaaactc
      421 agcctggata ccagctccag catgactggg gcgaagttga ggtggaggtt gccgggcaac
      481 ggtgcaaagt taactttgcg gttaatacgc tggggttctc ccgcagcttc catgtcttcg
      541 ccgcaccaaa acaggatgct gagcatacct acgaatcact ggttcgcgcc ttccgctact
      601 tcggtggttg tgtgaaaacg gtgctggttg ataaccagaa ggctgcggtg ctgaagaata
      661 acaacgggaa agtcgtgttc aactccggat tcctgttgct ggctgaccac tataacttcc
      721 tgccacgggc atgccgtcca cgcagggcca gaacaaaagg taaggttgag cggatggtga
      781 aatacctcaa ggagaacttc ttcgtccggt accgcaggtt cgacagcttc actcatgtca
      841 atcaacaact ggagcaatgg atagccgatg tggctgacaa acgggagctt cgccagttca
      901 aagaaacgcc ggaacagcgc ttcgcgctgg agcaggaaca tctgcagccg ttaccggata
      961 cagacttcga taccagttac ttcgatatcc gccatgtgtc ctgggacagc tatatcgagg
     1021 ttggtggtaa tcgttacagc gttcccgaag cgctgtgtgg tcagccggta tcgatacgaa
     1081 tatcgctgga tgacgagttg cggatctaca gtaatgagaa actggtggcc tcacatcgcc
     1141 tctgttcagc atcgtctggc tggcagacag tgccggagca tcacgccccg ctctggcagc
     1201 aggtcagtca ggtggaacat cgaccactga gtgcctatga ggagctgttg tgatgcatga
     1261 actggaagtc ctgctgagtc gcctgaaaat ggagcatctg agttatcacg ttgaaagcct
     1321 gctggaacag gcagctaaaa aagagctgaa ctaccgggag ttcctgtgca tggcgctaca
     1381 gcaggaatgg aacggcaggc atcagcgcgg tatggagtcc aggctgaagc aggctcgtct
     1441 gccgtgggtc aaaacgctgg agcagttcga ctttaccttc cagccgggca tcgaccgtaa
     1501 ggttgtccgg gaactggctg gtctggcgtt cgtggagcgc agcgaaaacg tgatcctgct
     1561 gggccctcct ggtgtcggaa aaactcatct ggccatagct cttggcgtga aagcggtgga
     1621 tgcgggacat cgggtactgt ttatgccact ggacagactg atcgcgacac tgatgaaagc
     1681 gaaacaggaa aaccggctgg agcgtcagct acagcaactg agttatgccc gggtgttgat
     1741 cctggatgaa ataggctatc tgccgatgaa cagagaggaa gccagtctgt tcttccggct
     1801 actgaaccgt cgatatgaaa aagcgagcat catactgacg tcaaacaaag ggttcgcaga
     1861 ctggggagaa atgttcggag atcacgtact ggcaacagcg atcctggatc ggctgctaca
     1921 tcactcaacc acgctgaata tcaaaggaga gagttaccgg ttaaaagaga aacgtaaagc
     1981 tggagtgctg accaaaaaca caacgccaat cagtgatgat gaaatggtgg aaagcggaca
     2041 gcatcagtaa cgaaagtatt tagcgggcat gaaaatggca aataacggtc aaacatcgtg
     2101 gcgttgacag ctgaacacct tcgggaagcc gaaggctggg gatggtgcgc cggacgcatg
     2161 gagcctgtcg gtgagtgccg tgaggatgcc ggaacatacc gctgtctgcc ggagccggaa
     2221 gcggtgctga cggaggcgga agaagaacgt ctgaacgaac tgatgacgcg ttacgacgcg
     2281 ctggaaaacc agtgtgagga atctgacctg ctggaagcag aaatgaagct gatgcgctgc
     2341 atggcgaagg tcagggcgtg gacgccggag atgcgtgccg gaagcggtgt ggtggtgtcc
     2401 tggcgttatg gcaacgtgtg tgtccagcgt ggtgtgcagt tgcgcagcga ggatgatgcg
     2461 gctgacgacg ctgaccgcac ggaacaggtg caggagaaag cgcccgtgga ggaaatcagt
     2521 ctgccactgc tgacgaaaat gtcttccgaa cgcacgctgg cagtccaggc agcactgatg
     2581 cagcaaccgg acaaatctct ggcactgctg gcatggacgc tctgccctga tgaatgtgtt
     2641 tggcagcgga gcgtacagta aaccagcaca aatcagcctg gaatgtaaac attattcgct
     2701 gaccagcgat gcgccatcgg ggaaggatgg tgccgcattc ctggcgctga tggcagaaaa
     2761 agcccgtctt gcagccctgt taccggaggg atggtcacgg gatatgacga cattcctgtc
     2821 actcagccag gaggtactgt tatccctgct cagtttctgc accgcgtgca gcatccacgg
     2881 cgttcagacc cgcgagcatg gtcacacgtc acgcagtccg ctggactcgc tggagagcgc
     2941 tatcggattc cacatgcgcg actggtggca gccgacgaaa gcaaacttct tcggacacct
     3001 gcaaaagccg cagattatcg atgccctgaa tgatgccgga ctgtccggtg ctgcgcggga
     3061 cgcggagaaa atgaagaaag gcgatgccgc tgaacatgca gagttccaca tgaaagacaa
     3121 ccgctgggtg cctggctgga tgtgtacacc acgtccacag gcagagacgg aaaccacgga
     3181 gtaccgtgac gaccaggcag aagccgcctg accgccagtc ataaaccatt tatcacgccg
     3241 ccctgccgga gacggggcgg cagcaaggga gataccgtga tgaaaactga actgaccctg
     3301 aatgtattac agaccatgag cgcacaggaa tatgaagata tccgggctgc cggaagtgat
     3361 gaacgtcgtg aactcacaca tgccgtgatg cgggagctgg atgcgccgga taactggacg
     3421 atgaacggcg agtatggcag cgagttcggc ggctttttcc ccgtccaggt ccgtttcacg
     3481 cctgcccacg aacgttttca cctggcgtta tgttcaccgg gcgatgtctc tcaggtctgg
     3541 gtgctggttc tggtgaatgc cggtggtgag ccgtttgcgg tggttcaggt acagcggcgg
     3601 tttgcaccgg aagccgtcag ccattcactg gcactggcgg cgtcactgga cgcgcagggg
     3661 tacagcgtca gtgacatcat ccatatcctg atggcggaag gaggtcaggc atgagcgcac
     3721 gttcacaggc actggttccc ctcagcgcag agcaacaggc cgcatggcgg gcggtggcgg
     3781 agacagaaaa acgccgccac cagggaaaca cgctggcgga atatccgtat gcgggggcgt
     3841 ttttccgctg tctgaacgga agccgcagga tatcgctgtc tgacctgcga tttttcatgc
     3901 cgtccctgac cgcagaagaa ctgcgcggca accgttctca gtggctgtat gccgtcgatg
     3961 tgctgattga aactcagggc gaggtctgcc ttcttcccct tcccggcgat gcagcagagc
     4021 ggctgttccc gtcggtgcgg ttccgtgtca gggagcgcag tcgccacaaa tccgcactgg
     4081 tcatgcaaaa gtacagccgc cagcaggcga gggaggcaga gcagaaagcc cgtgcgtacc
     4141 aggcgctggt cgctcaggct gaaatcgaac tggcattcca ttcgccggaa acagtcggaa
     4201 gctggcatgc acgctggagt gaccgggtgg cagagcatga cctggaaacc ctgttctggc
     4261 agtggggtga gcgttttccg tcactggccg gaatggaacg ctggcagtgg caggatatgc
     4321 cgttctggca ggttattgcg gaagccagtc ttgcagcaag agaggccggt cacgctgtcc
     4381 gtgagatgga gcggtggatg gtgccgaaca aactccggga ggcggcgtga tgcggcaaca
     4441 atcacacaga ttacccgtaa acagcctgaa tgagcgggtt attttcagga aaagtgagtg
     4501 tggtcagcgt gcaggtatat gggctatgat gtgcccggcg cttgaggctt tctgcctcat
     4561 gacgtgaagg tggtttgtta ccgtgttgtg tggcagaaag aagatagccc cgtagtaagt
     4621 taattttcat taaccaccac gaggcatccc tatgtctagt ccacatcagg atagcctctt
     4681 accgcgcttt gcgcaaggag aagaaggcca tgaaactacc acgaagttcc cttgtctggt
     4741 gtgtgttgat cgtgtgtctc acactgttga tattcactta tctgacacga aaatcgctgt
     4801 gcgagattcg ttacagagac ggacacaggg aggtggcggc tttcatggct tacgaatccg
     4861 gtaagtagca acctggaggc gggcgcaggc ccgccttttc agggctgatg ctggtctgac
     4921 tgcactgaag cgcctttata aaggggctgc tggttcgccg gtggcccctt tctccttgct
     4981 ggcattgtac gggcatgaac aaccagactg ccaggaaggc cgtttccctc cgggaacggg
     5041 gatgaacgtg cgcctgcggc gcccgttctt ttcccccgcc ttctctgttt atgacttacc
     5101 agaaatcaca tcaccttccc tttgattatt ctctttttcc tcctgctctg actctgatta
     5161 ctgctctgac tctgattact
//
LOCUS       DAEEKQ010000229         5174 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.232, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000229 DAEEKQ010000000
VERSION     DAEEKQ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5174
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.232"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            12..428
                     /gene="soxR"
                     /locus_tag="KLK57_003911"
     CDS             12..428
                     /gene="soxR"
                     /locus_tag="KLK57_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001129347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HBE0894157.1"
                     /translation="MAKRSGVAVSALHFYESKGLITSIRNSGNQRRYKRDVLRYVAII
                     KIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREELDRRIHTLVALRDELDG
                     CIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            974..2323
                     /gene="ghxP"
                     /locus_tag="KLK57_003912"
                     /gene_synonym="yjcD"
     CDS             974..2323
                     /gene="ghxP"
                     /locus_tag="KLK57_003912"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HBE0894158.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            2475..4124
                     /locus_tag="KLK57_003913"
     CDS             2475..4124
                     /locus_tag="KLK57_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HBE0894159.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(4278..5174)
                     /locus_tag="KLK57_003914"
                     /pseudo
     CDS             complement(4278..5174)
                     /locus_tag="KLK57_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
BASE COUNT         1120 a         1202 c         1395 g         1457 t
ORIGIN      
        1 cccccggcga agtggcgaaa cgcagcggtg tggcggtatc ggcgctgcat ttctatgaaa
       61 gtaaagggtt gattaccagt atccgtaaca gcggcaatca gcggcgatat aaacgtgatg
      121 tgttgcgata tgttgcaatt atcaaaattg ctcagcgtat tggcattccg ctggcgacca
      181 ttggtgaagc gtttggcgtg ttgcccgaag ggcatacgtt aagtgcgaaa gagtggaaac
      241 agctttcgtc ccaatggcga gaagagttgg atcggcgcat tcatacctta gtggcgctgc
      301 gtgacgaact ggacggatgt attggttgtg gctgcctttc gcgcagtgat tgcccgttgc
      361 gtaacccggg cgaccgctta ggagaagaag gtaccggcgc acgcttgctg gaagatgaac
      421 aaaactaaag cgccacaagg gcgctttagt ttgttttccg gtctttgtct ttctctctat
      481 cccgctggta cacaggaggg tttcccccga cgtcaacaca ccccattcga gcacgtggtg
      541 gaggttccgg ttggtgttga tgctttaatt gtatgtcacc ggcatttctt cgccagtgta
      601 aaagtatact ttttatccgc aatatttttg tcatctcaga cgatttttta tcgcaatcct
      661 gaacggtata cggctcgata acgctgcaat cttgcgcacc gacgataacg tttgcgcatc
      721 aattgcctgg tttttcatcg tcaagacaat aaaagagaaa aaagcagcaa acttcggttg
      781 aaaaagccgc tatgatcgcc ggataatcgt ttgctttttt taccacccgt tttgtatgcg
      841 cggagctaaa cgtttgcttt tttgcgacgc agtaaattgt cgcaaacctg gagcaggaag
      901 ataacgtttc tctggcaggg gattgtccgc cacgcatctt gacgaaaatt aaactctcag
      961 gggatgtttt cttatgtcta cgccatcagc gcgtaccggc ggttcactcg acgcctggtt
     1021 taaaatttca caacgtggaa gcactgtccg tcaggaagtg gttgccgggt taacaacgtt
     1081 tctggcgatg gtctactcgg tcatcgtcgt tccaggtatg ttgggtaaag caggcttccc
     1141 gcctgcggca gttttcgttg caacctgtct ggttgccgga ctcggttcta tcgtgatggg
     1201 tctgtgggct aatctgccgt tggcgattgg ttgcgccatc tccctgacgg cgtttaccgc
     1261 attcagcctg gtgctggggc aacatattag cgtacctgtc gcgctgggtg ccgtgttcct
     1321 gatgggtgtg ctgtttacgg taatttctgc cacgggtatc cgtagctgga ttttgcgcaa
     1381 cttgccgcac ggtgtggcgc acggcacggg gatcggtatc ggtctgttcc tgctgctcat
     1441 tgccgctaat ggtgtcggtc tggtgattaa aaacccgctt gatggtctgc ccgttgcgct
     1501 gggtgatttc gcgaccttcc cggtgattat gtcactggta ggtctggcgg tgatcatcgg
     1561 cctggaaaaa ctgaaagtcc ctggtggcat tctgctgacc attatcggta tctcaattgt
     1621 cggtttgatc ttcgatccta acgtccattt ctccggcgtt ttcgccatgc cttcattgag
     1681 cgatgaaaac ggcaattcac tgattggcag cctggacatt atgggcgcgc tgaatcctgt
     1741 agtcctgcca agcgttctgg cgctggtgat gacggcagta tttgatgcca ccggaactat
     1801 ccgtgccgtc gccggccagg cgaacctgct ggataaagat gggcagatca tcgacggtgg
     1861 gaaagcactg accactgact ccatgagcag cgttttctct ggcctggtgg gtgctgctcc
     1921 ggcagcggta tacatcgagt ctgcggcggg tacggcggcg ggcggtaaaa ccggtttgac
     1981 ggctatcacc gttggcgtgc tgttcctgct gattctgttc ctctctccgc tctcttacct
     2041 cgttccgggg tatgcaacgg ctccggcgct gatgtacgtt ggcctgctga tgctgagcaa
     2101 cgtggcgaaa atcgactttg ctgattttgt tgatgcgatg gcgggtctgg ttacggcggt
     2161 attcatcgtg ctgacctgta acatcgtaac aggcatcatg atcggcttcg cgactctggt
     2221 gattggtcgt ctggtttccg gcgaatggcg caagttgaac atcggtacgg tcgttatcgc
     2281 cgtggcgctg gtgaccttct atgcgggtgg ctgggctatc taatctttca tcggattctg
     2341 aaaacgggtg gcaatggctg cccgttttta ttttctccgc acattgtgtg agcttttgcg
     2401 atattctgaa aaaatgagaa ttcaggcata acgcctttct cagatcataa aaaaacatcg
     2461 caaacaggga acgcatggaa atcttcttca ccatactgat aatgaccctc gtggtctcac
     2521 tgtccggggt ggtcactcgt gtcatgccct ttcagatccc gcttccgctt atgcaaatcg
     2581 ccatcggtgc gctactggcg tggccgacgt ttggtttgca tgtggagttt gatcctgaac
     2641 tctttttagt cttgtttatc ccgccgttgc tgttcgctga tggctggaaa acgccgaccc
     2701 gtgaatttct cgaacatggt agagagattt tcggcctcgc gctggcgctg gtggtggtca
     2761 ccgtggtcgg cattggcttc cttatttact gggtggtgcc gggcattccg ctgatccccg
     2821 cctttgcgct ggcggcggta ctttctccga ccgatgctgt ggcgctctcc gggattgttg
     2881 gcgaagggcg catcccgaaa aaaatcatgg gcattttgca gggcgaagcg ttgatgaatg
     2941 acgcctccgg cctggtgtcg ttgaagtttg ccgtggcagt ggcgatgggg acgatgatct
     3001 tcaccgtcgg cggtgcgacg gtcgaattta tgaaagtagc cattggtggt attcttgccg
     3061 gttttgtggt gagctggctg tatggtcgct cgctgcgatt cctcagccgc tggggcggtg
     3121 atgaacccgc gacgcagatc gttctgctgt tcttgctgcc attcgcttct tatctgattg
     3181 ccgaacatat tggcgtttcc ggcatcctcg ctgcggttgc cgccgggatg accatcaccc
     3241 gctccggtgt gatgcgccgt gcgccgctgg caatgcgcct gcgcgcaaac agcacctggg
     3301 cgatgctgga atttgtcttt aacggcatgg tgttcctgct gttaggtctg cagctgccgg
     3361 gtattctgga gacgtcgctg atggcggcag aaatcgaccc taacgtcgaa atctggatgc
     3421 tgtttaccga tattattctg atatatgcgg cgctgatgct ggtccgtttc ggctggctgt
     3481 ggacgatgaa aaagttcagc aaccgcttcc tgaagaagaa gccgatggag tttggttcgt
     3541 ggaccacacg agaaatcctg atcgcgtctt tcgccggggt gcgtggggcg atcactctgg
     3601 ccggtgtgct ctctatcccg ctgctcttgc cggatggtaa cgtcttcccg gcgcgctatg
     3661 agctggtgtt cctggcggct ggcgtcattc tcttctcgct gtttgtcggc gtggtgatgt
     3721 tgcctattct gctacaacac attgaagtcg cggaccattc gcaacaattg aaagaagaac
     3781 gtattgcgcg agcggcaacg gcagaagtgg cgattgtggc gatccagaaa atggaggagc
     3841 gtctggcggc ggataccgaa gagaatatcg ataaccagct gcttacggag gtcagttctc
     3901 gcgtcattgg taacctgcgt cgtcgcgccg atggacgtaa cgacgttgaa agttccgtgc
     3961 aggaagagaa ccttgagcgt cgcttccgtc tggcggcatt gcgttctgaa cgtgctgaac
     4021 tgtaccacct gcgcgccacg cgggagatca gcaacgaaac gctgcaaaaa ttactgcacg
     4081 atctcgattt gcttgaagcg ttgctaattg aggaaaatca gtaagccgaa agcgacgtaa
     4141 ccaggcccga ggtaatctga aaaaggtgca atcttttgca cctttaacag gttagctcgg
     4201 gctaacattc atgattctaa aacaaataag ttatctctct tgtaaagtca attcattggc
     4261 tgttcttcga cttaacctca atctgcgtgt tgcataatat ctgcagtaat cttcatagcg
     4321 actgcattaa tattatcctc agtttgtgac atgccagtat gagaggtaca atcttcttca
     4381 aagaatttct gtagtgttac cagggtatca ctatcaatgg ggatattttc ctccccacgg
     4441 agtatggaaa tgcccattcc ctggacgcta gcctcatcaa aattaacccg atagagacgg
     4501 gtattaaaca tacaggcttt tgttaaaata gaacagttga aatggcagtt gcttaaattg
     4561 gcattattaa aattaacatt agccatatta gagtcggtac atataaccat ttcgaggaat
     4621 gtatttgtta gccacgtaca aaagagtttg ctatcaatta agtttatatt ctggaggatt
     4681 gctttgttca gattgacgcg agacagatca gcatgttcaa agttaattaa atcgagttta
     4741 gagaaactaa ggtcggcttt aaataaatta acatatggcg ctattacttt ataaagattt
     4801 gcctcagcca tgtacgccat ataaaaattt gaatagtcaa gaaaggtatt tttaatattg
     4861 gctctattca ttgctgcatt gtagaattga acatgcataa cggagcatgc atttagagtt
     4921 ccgtcggcca tttttatctg agcgaaattt gagtcatcga gaatacaatt atcaaaaagt
     4981 atttttccat tatggcgtaa taaaaattcg ttttatacat attacagtcc tggaaaatag
     5041 cgttttgtag tctgcaacct gcaaaattcg aattggataa atcttcatct ttaatcacga
     5101 tacctttcag gctaaggtgt gaaaaatcgt aaccaccatt tactgtctgt ggaatttcga
     5161 tagtattatt atgg
//
LOCUS       DAEEKQ010000230         5166 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.233, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000230 DAEEKQ010000000
VERSION     DAEEKQ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5166
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.233"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            375..584
                     /locus_tag="KLK57_003915"
     CDS             375..584
                     /locus_tag="KLK57_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894160.1"
                     /translation="MAKLGENTPHIIDATVDFMASSQAFREYLKKLPPDNTIPPALTD
                     EHVPLYIRRLEYYRLLYRPKQTESY"
     gene            complement(650..1183)
                     /locus_tag="KLK57_003916"
     CDS             complement(650..1183)
                     /locus_tag="KLK57_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894161.1"
                     /translation="MASTEEKLLTLLLSVEHLQHTAIEQQKAVADSLAVARETLRGYR
                     DISDGVAKQVRDEVRNEVQKLDVAGLIGERISQDFQRLEESVANMKANADALNRDIAT
                     IHSNIVQEYNTLKGYSWKWLGGVSVAFLVMFCVLGWGMKTILDRNYNATAAVYQKVVT
                     LEDKLVEKPVTGKSRKN"
     gene            complement(1186..2112)
                     /locus_tag="KLK57_003917"
     CDS             complement(1186..2112)
                     /locus_tag="KLK57_003917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HBE0894162.1"
                     /translation="MATYHLSVKFGGKGQAANHADYIERKEKYRDRQDLEYSAHGNMP
                     EWARDNPSHFWQAADQFERANGSTYRELEIALPRELTPEQRLELVQDFVRQEAGERHA
                     WSFAIHNPKASIDGGEQPHAHIMMSQRVNDGIDRTPEQYFRRYNARYPERGGAKKDSG
                     SLTPTQQKEQLRELRKRWEVKHNEHMRKHGFERGFIDCRTLKEQGIERRPEVHLGFEA
                     AGRLDDAQRHDIMQKRSEPDYSRHLEQARMVGHDIREWLDTPVKQHELVRRLQHDVAR
                     EHGFDMRDGVSRENRTEPERVKSPSRGFSMGF"
     gene            2289..2687
                     /locus_tag="KLK57_003918"
     CDS             2289..2687
                     /locus_tag="KLK57_003918"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894163.1"
                     /translation="MRISNIEWLKKRIGFIRKLGEQTARQRQIIDLIDNEAGLTEQER
                     KLLHVLATAEKNDLQAQESERKQAVQKRIEGKKQRRERNHRLFLAAGLLIEAGLVDTK
                     TGELCYKKDRILQALKELKYDLETSPNPDA"
     gene            complement(2891..3805)
                     /locus_tag="KLK57_003919"
     CDS             complement(2891..3805)
                     /locus_tag="KLK57_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HBE0894164.1"
                     /translation="MSAALQYFDENLPHRPYHTDDLAFGLRISGKGRALLARYIQQNQ
                     PHAQFWLVFDVDREGAAIDWSDRNAPAPNITVKNPVNGHAHLLYALNIAVRTAPDASV
                     KALKYAAAVERSLCEKLCADVNYSGLICKNPFHLEWLVMEWREEAYTLDELADYLDLS
                     ASERRSIDKHYGMGRNCHLFEMTRKWAYRAIRQGWPAFSQWLEAVIQRVEMYNASLPV
                     PLSLAECRAIGKSIAKYTHRNFTPETFAQYVADTHTPEIQAARGRKGGSKSKRSTVAT
                     SARTLKPWEALGISRAWYYQLKKRGLVE"
BASE COUNT         1433 a         1346 c         1105 g         1282 t
ORIGIN      
        1 aaaaaaaacc tctgcgagcg cagagggggt aaggaatgtt cccggtcata atatgtcgat
       61 agtgtttata tacatacaga acaagaacaa caaccagcca actaataaat attttatttt
      121 ttagagctga aaccttaaag ctcacttcca ttccatacat tgttataaac cttatgttga
      181 aaactgaaag taagtacggc agacttatta agttgcgtgc agtcttttca cgttatacaa
      241 acaagaacac ctgcgaagtt atttctcctt acatatcccc ctatgccaac gggggatttt
      301 ttctggttca ctaacgcata aataatagtg acaccctatc attcacaaac cgtgataata
      361 taaggattat catcatggca aaattaggtg aaaacacccc acatattatc gatgctactg
      421 tagattttat ggcatcaagc caggcatttc gtgagtatct gaaaaaactt ccccctgata
      481 acacaatacc gccagctctt actgatgagc acgtaccgtt atatatacga aggctggaat
      541 actatcgtct gctttatcga ccaaagcaga cagaatcata ttgaaaaaaa cggtgaacgg
      601 ttcgcgaaat cctttcgcgg gtcgtgaggc gttggacaag cgaagcgcgt cagtttttcc
      661 ggctcttccc cgtgacaggt ttctccacca gtttatcttc cagtgtcaca actttctgat
      721 acaccgccgc tgtggcgttg tagttcctgt cgagtattgt tttcattccc catccgagca
      781 cacagaacat aaccagaaaa gccactgaca ccccacccag ccatttccag ctataccctt
      841 taagggtgtt gtattcctgt acgatattgc tgtgaattgt cgcaatatcc ctgttcaggg
      901 catccgcgtt tgccttcatg tttgcgactg actcttccag ccgctgaaaa tcctggctga
      961 tgcgctcacc tatcagccct gccacatcca gcttctgcac ctcatttcgg acttcatccc
     1021 tgacctgttt tgccacgccg tcagaaatat cccgatatcc gcgaagtgtt tcccttgcca
     1081 cagccagaga atcagcaaca gctttctgtt gctctattgc ggtatgttgc agatgctcca
     1141 ccgataacag gagcgtgagt aatttttctt ctgtgctggc catgttcaga atcccataga
     1201 aaatccacgt gacggtgatt taacccgttc aggttcagtt cggttctctc tgctcactcc
     1261 gtctctcatg tcaaagccat gctcccgcgc aacatcatgt tgcagccttc tgaccagttc
     1321 atgttgctta accggtgtat ccagccattc cctgatgtca tgcccgacca ttcgcgcctg
     1381 ttcaagatgg cgggaatagt ccggttcaga ccgtttttgc atgatgtcat gacgctgggc
     1441 atcatcaagt cgtccggcag cttcaaaacc cagatgcact tcgggacgtc gttcaatgcc
     1501 ctgttctttc agtgtccggc agtcaatgaa gcccctttca aagccatgct ttctcatatg
     1561 ttcgttgtgc ttcacctccc agcgttttcg caattcccgc aactgctctt tttgctgagt
     1621 gggagtcagg cttccggaat cctttttcgc acctccccgc tcgggatacc tggcgttata
     1681 gcgccggaaa tactgttccg gtgtacggtc aatgccatca ttcacacgct gactcatcat
     1741 gatatgcgca tgaggctgtt cgccgccgtc aatgctcgct ttcggattgt gaatggcaaa
     1801 actccaggcg tgccgttcgc cggcttcctg acgtacaaaa tcctgcacca gttcaaggcg
     1861 ctgttccggt gtcagttcac ggggtaatgc aatttcaagt tccctatagg tcgaaccgtt
     1921 cgcacgttca aactggtcag ccgcctgcca gaaatgggaa ggattatccc tggcccattc
     1981 cggcatgttt ccgtgtgcgg aatattccaa atcctgacgg tcgcggtatt tctctttgcg
     2041 ctcgatgtaa tcggcgtggt ttgcagcctg ccccttgcca ccaaatttaa cgctgaggtg
     2101 ataggttgcc acggtttcag ccctccgcag gagcgcactg tttctcgacg aaggagagaa
     2161 acatataagt gcgcttcctt ctaattttta gaattagcac ggaagatacc cccctgcacc
     2221 caccgaccgc aagcgatata ctgcacgacc acaacacacc gcaaaaaaca ccagggagca
     2281 tcacgaacat gcgcatcagc aacatcgaat ggctgaagaa acggatcgga ttcatcagga
     2341 agctcggaga acagaccgca cgacagagac agattattga cctgatcgac aacgaagccg
     2401 gactcacaga acaggaacgc aaacttctcc acgtactggc aacagcggaa aaaaacgact
     2461 tacaggcaca ggagagcgaa cgaaaacagg cggtccagaa acgaatcgag ggaaaaaaac
     2521 aacgacggga acgcaatcac cggctctttc tcgcggctgg gctactgatc gaagcaggac
     2581 tggtagacac aaaaacaggc gagctatgct ataaaaaaga caggatatta caggcactta
     2641 aggaacttaa gtacgatctg gagacatccc caaacccaga cgcgtaagtg cctccatccg
     2701 tgttccgatt ccctggtgct tcctcgctca ttcgctcgct acgctcggtc atgagactgc
     2761 ggcgagcact accaccacaa tcacgcggaa agccgcgcca ttgcagggct gaacggggaa
     2821 aaacggcaaa aaacctgctg ctcaaaaaga cgccagaaag gcgctgttat ctgatatagg
     2881 cttatttggt ctactctacg agacctcgtt ttttcagttg gtaataccag gcgcgactga
     2941 tccctaatgc ctcccacggt ttcagcgtgc gtgcagatgt agctactgtg ctgcgcttag
     3001 acttagaacc gcccttgcga ccgcgtgcag cctgaatttc tggcgtgtgc gtatcagcca
     3061 catactgtgc gaaagtttcc ggcgtgaagt tcctgtgcgt gtatttcgca atgctcttgc
     3121 caatagcccg acattcagcc agtgaaagcg gaacgggaag cgatgcattg tacatttcga
     3181 cacgctggat caccgcttca agccactgtg agaacgctgg ccagccctga cgaatcgccc
     3241 tgtaagccca tttgcgcgtc atttcgaaca ggtggcagtt tcgccccatc ccgtaatgtt
     3301 tatcgatgct acgacgctct gaggcgctca aatcgagata atcagccagt tcatcgaggg
     3361 tataggcttc ctcgcgccac tccatcacca gccattccag gtggaacgga tttttgcaaa
     3421 tcaggccgct gtagttcaca tccgcacaaa gtttctcaca cagcgaacgt tcaaccgctg
     3481 cggcatattt cagcgcctta accgaggcat caggcgcggt tctcacggcg atgttgagtg
     3541 cgtagagcag gtgagcgtgt ccgttcacag gatttttaac ggtgatgttc ggcgcgggag
     3601 cgttccggtc gctccagtca atcgcagccc cctcgcggtc aacgtcaaaa accagccaga
     3661 actgcgcatg aggctggttc tgctgtatgt accgcgcaag aagcgcacgc cctttgccgg
     3721 agatgcgaag accaaaagcg agatcatccg tgtgataggg acgatggggt aaattttcgt
     3781 cgaagtattg aagcgcggcg ctcatgtgtt acgattctct ccgggttatg cgacctacac
     3841 gccctctgta tttcatgttg aatcttgccg gatctggaca tgatttcagg ggcgtttgtt
     3901 tttcagtcgc ttacactaca cccaacggca aatcctgtcg aaccttcccg aaaacccgat
     3961 ccatttacca taactcccgt tgtacgtacc cattaaaacg ccctcagtcg ctttctgacg
     4021 caaaaaacac aaaaagacaa ggaaacacca gggtaaacat aaaaaatgcg tcagaatcgt
     4081 ttttagcgcg ttgcataact atgcacgaaa gcgatgcgcg atcggtttcg tgcgttctga
     4141 acaccgaacg gctcaaaacg acaagttttc tcccctggtg cgtggcgctc tgacggggtt
     4201 tcctgtttcc aaacttcaca tctcgccagg acaaaaaaaa ccgacctggg gtcggtggca
     4261 agaatcagag tctatgggaa atattttagt tatcggatgc tcaatattca caaacgcaaa
     4321 aaatgcctca gtgatgccct aaaacaacct ttccagaagg agtccgcgct ttacgatgca
     4381 cggaagagat aaaaaacccc acaaaagtgg ggaaccaaga gaaacaacaa ttacaggttg
     4441 ttggttgtgt tcgaacaacg atgaagtagc tacgctaaat aataattacc gtacaggccc
     4501 tacatattca acactaaatt taagatcctg acgaaggatt tataaatgca cgaaaacaca
     4561 tagacagaca aattcactaa attcatacac aaaaacaagt gtagttttct aagtaaatta
     4621 gattcgacaa ccaattagtt tatccacatc gcattccact cttgctacac caatcacatt
     4681 tcttgatatg taatagcaga aaaaataaaa ccagacacca atagaccaaa aaacaaacat
     4741 tcaacaagaa acattttgaa caatatcaca aaactgtcac aaccatcatc agactattga
     4801 aaaaaaactg gttgctgttt tataaaagcg actgtaacaa cctccactct atagttccat
     4861 tagtttaaag tgtcacacaa actgagcatc catttgtttt ccttactttt gcccctgtat
     4921 tccaaggggt ttttttataa cttaaagatt agcgcaccac acaaaaacaa taaaaaaaac
     4981 gacaagttat acaataaaac cagtactatc catacagcta attacatata aatactatta
     5041 aaccatctgg acattaaaaa taaactctta taacataaaa ttaatgcggt tccggtacca
     5101 tatgtcaggg ataatgtgca aatggaggta tcggaactgc atttcagttc attattaatt
     5161 aatgac
//
LOCUS       DAEEKQ010000231         5070 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.234, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000231 DAEEKQ010000000
VERSION     DAEEKQ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.234"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            175..1857
                     /locus_tag="KLK57_003920"
     CDS             175..1857
                     /locus_tag="KLK57_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HBE0894165.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGAPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1909..3066
                     /locus_tag="KLK57_003921"
     CDS             1909..3066
                     /locus_tag="KLK57_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HBE0894166.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPLELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHIQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3032..3154)
                     /locus_tag="KLK57_003922"
     CDS             complement(3032..3154)
                     /locus_tag="KLK57_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894167.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            3363..5048
                     /locus_tag="KLK57_003923"
     CDS             3363..5048
                     /locus_tag="KLK57_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HBE0894168.1"
                     /translation="MITIPITFYMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQTELIALSNAESLQRQELRDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHDHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1516 a         1080 c         1020 g         1454 t
ORIGIN      
        1 atcggcgaga aattacttaa tagaacagaa agtaatgtca acgctttatg gactgttatt
       61 tccctttttt tagctaaatc tgctatctct ttatgtgact aacttcactt acatccactt
      121 atttctcttc gtaaaattac ttcggaatta agtacaataa gaagaggaac atttatgaag
      181 tctgcattaa agaaaagtgt cgtaagtacc tcgatatctt tgatactggc atctggtatg
      241 gctgcatttg ctgctcatgc ggcagatgat gtaaagctga aagcaaccaa aacaaacgtt
      301 gctttctcag attttacgcc gactgaatac agtaccaaag gaaagccaaa tattatcgta
      361 ctaaccatgg atgatcttgg ttatggacaa cttccttttg ataagggatc ttttgaccca
      421 aaaacaatgg aaaatcgtga agttgtcgat acctacaaaa tagggataga taaagccatt
      481 gaagctgcac aaaaatcaac gccgacgctc ctttcattaa tggatgaagg cgtacgtttt
      541 actaacggct atgtggcaca cggtgtttcc ggcccctccc gtgccgcaat aatgaccggt
      601 cgagctcccg cccgctttgg tgtctattcc aataccgatg ctcaggatgg tattccgcta
      661 acagaaactt tcttgcctga attattccag aatcatggtt attacactgc agcagtaggt
      721 aaatggcact tgtcaaaaat cagtaatgtg ccggtaccgg aagataaaca aacgcgtgac
      781 tatcatgaca acttcaccac attttctgcg gaagaatggc aacctcaaaa ccgtggcttt
      841 gattacttta tgggattcca cgctgcagga acggcatatt acaactcccc ttcactgttc
      901 aaaaatcgtg aacgtgtccc cgcaaaaggt tatatcagcg atcagttaac cgatgaggca
      961 attggcgttg ttgatcgtgc caaaacactt gaccagcctt ttatgcttta cctggcttat
     1021 aatgctccgc acctaccaaa tgataatcct gcaccggatc aatatcagaa gcaatttaat
     1081 accggtagtc aaacagcaga taactactac gcttccgttt attctgttga tcagggtgta
     1141 aaacgcattc tcgaacaact gaagaaaaac ggacagtatg acaatacaat tattctcttt
     1201 acctccgata atggtgcggt tatcgatggt cctctgccgc tgaacggggc gcaaaaaggc
     1261 tataagagtc agacctatcc tggcggtact cacaccccaa tgtttatgtg gtggaaagga
     1321 aaacttcaac ccggtaatta tgacaagctg atttccgcaa tggatttcta cccaacagct
     1381 cttgatgcag ccgatatcag cattccaaaa gaccttaagc tggatggtgt ttccttgctg
     1441 ccctggttgc aagataagaa acaaggcgcg ccacataaaa atctgacctg gataacctct
     1501 tattctcact ggtttgatga ggaaaatatt ccattctggg ataattacca caaatttgtt
     1561 cgccatcagt cagacgatta cccgcataac cccaacactg aggacttaag ccaattctct
     1621 tatacggtga gaaataacga ttattcgctt gtctatacag tagaaaacaa tcagttaggt
     1681 ctctacaaac tgacggatct acagcaaaaa gataaccttg ccgccgccaa tccgcaggtc
     1741 gttaaagaga tgcaaggcgt ggtaagagag tttatcgaca gcagccagcc accgcttagc
     1801 gaggtaaatc aggagaagtt taacaatatc aagaaagcac taagcgaagc gaaataacta
     1861 aaccttcatg cggcggattt ttccgccgcc ttattgagcg agatagcgat gcacgttaca
     1921 gccaagccct ccagttttca atgtaatctc aaatgtgatt actgttttta ccttgaaaaa
     1981 gagtcgcagt ttactcatga aaaatggatg gatgacagca ctctgaaaga gttcatcaaa
     2041 caatatatcg aagcgtctgg caatcaggtc tattttacct ggcaaggcgg tgaacccact
     2101 ctggctggcc tggatttttt ccgtaaagtt attcactatc aacaacgcta tgcaggccaa
     2161 aaacgtattt ttaatgcatt acaaacgaat ggcattttat tgaataatga atggtgtgcc
     2221 tttctcaaag aacatgaatt tctggttggt atctcgatcg atggccccct ggagttacat
     2281 gaccgttaca gacgcagtaa ttcaggtaac ggtacttttg caaaagtgat agcagccatc
     2341 gagcgtctga aatcatatca agtagagttt aatacgttaa ccgtcattaa taacgttaat
     2401 gtccattacc ctcttgaggt ttatcatttt ttaaaatcta tcggcagtaa acatattcaa
     2461 tttatcgaat tgctagaaac cgggacgccg aatattgatt tcagtggtca tagtgagaac
     2521 acattccgta tcattgattt ttctgtgcct cccacggctt atggcaagtt tatgtcaacc
     2581 atttttatgc aatgggttaa aaacgatgtg ggtgaaattt tcattcgtca gtttgaaagc
     2641 tttgtcagcc gttttttggg gaatgggcat acaagttgta ttttccagga gtcctgcaag
     2701 gataatctgg ttgttgaaag taatggagac atttacgaat gcgaccattt tgtctatcca
     2761 cagtacaaaa ttggaaacat taataaatct gaactcaaaa cgatgaacag tgtacaactg
     2821 acagcgcaaa aaaaacggat ttcagcgaaa tgtcagcaat gtgcatataa acctatctgc
     2881 aatggcggtt gtcctaagca tcgtattact aaagtaaaca atgagactgt ttcttatttt
     2941 tgcgaaggtt ataaaatcct tttttcaacc atggtacctt atatgaacgc catggttgag
     3001 ttagctaaga acagagtacc gctttaccac attatggatg ttgcaaaaca aatggagaat
     3061 aattaataac gctctctttt aactccattc ttagcctcct tcttatgacc ttaacaaggc
     3121 acccgacata aattcctgtc gggtgtttcg tcatcatcat cttcccttgc gaaaatcctg
     3181 gcttacgtgg tatgcgtcag ggcattagca atacccttga ttatcaatag cataaccata
     3241 accataacca ctgaaaatta aaaatttcta tatatttcat attgttaaat gatacttaaa
     3301 taaatttcat cagtaaatca ttgccataga taatcattgg atggcacaat caattttcgc
     3361 taatgataac cattcccatt acgttttata tgttgatagc aaagtatctc tgtctgctga
     3421 agcccttttg gctgcgtaag aataataaaa cttcagtgtt gttaatcatt attatcctcg
     3481 caatgatcct tggcgtggtt aaaattcagg tctggttaaa cgactggaat aatgattttt
     3541 tcaatgcgct aagccagaaa gaaaccgata agctttggca gcttgttctt tggttccctg
     3601 ccctgctggg gatctttgta ttaatatccg taaacaaaac atggttaatt aaactactga
     3661 ctattcgctg gcgcgaatgg ttaacagatt actaccttaa tcgctggttt gcagataaaa
     3721 actactactt cacgcagatc tacggcgagc ataaaaatac agataaccct gaccaacgta
     3781 tcgctgaaga cattctctta ctgatcagca aaacgctcag tctttcattt ggcttcatcc
     3841 agtcacttag catgctgatc acctttaccg ttattctttg gcaaagcgcg ggtacgctct
     3901 cattcactgt tggtggaaca gaatggaata tccaggggta tatggtctat accgtcgtgc
     3961 tcattgtgat cggtggaact ttatttaccc ataaggtggg taaacgtatt cgtccgctta
     4021 atgtggaaaa acaacgtagc gaagcgacat ttcgaactaa tcttgtgcag cataataagc
     4081 agacagaact gattgcgcta agcaatgctg agtctctaca acgccaggag ttgcgcgaca
     4141 attttcatac cattaaagag aactggcatc gtttaatgaa tcgtcagcgg tggcttgatt
     4201 actggcaaaa tatttattcg cgctcgctta gcgttcttcc ctactttctg ttattgccgc
     4261 agtttattag cgggcagatc aatctgggcg gactgatgaa atcgcgccag gcatttatgc
     4321 tagtatcgaa caatttaagc tggtttattt ataaatatga cgaacttgct gaactggctg
     4381 cggttatcga tcgcttgtat gagttccatc aactcactga acagcgccct acgaataagc
     4441 ctaaaaattg ccaacatgcg gtacaagtgg ctaatgcgag tattcgtacg cctgataata
     4501 agatcatatt agagaacttg aactttcatg tttcgccagg caaatggcta ttactgaaag
     4561 gctactctgg tgcgggaaaa accacactgc ttaaaacatt atcccactgc tggccgtggt
     4621 ttaaaggtga tatttcttct cctgctgaca gttggtatgt gtcacaaaca ccgttaatca
     4681 aaagcggctt actgaaagag attatttgta aagcacttcc cctgcccgta gacgataaat
     4741 cgttgagcga agtactgcat caggttggtc tggggaaatt ggctgcgcgt attcatgacc
     4801 acgatcgctg gggagatatt ctttccagcg gcgaaaaaca acgtatcgcc ctggcacgat
     4861 taattttacg acgtccgaaa tggatatttc ttgacgaaac tacctctcat cttgaggaac
     4921 aagaggctat acgcttactg cgtttagtgc gtgaaaaact acccacaagc ggcgtcatta
     4981 tggttacaca tcaacccggt gtctggaacc tggctgatga tatttgtgac attagcacgg
     5041 ttttataaca acaataaaag ccctgctgta
//
LOCUS       DAEEKQ010000232         5048 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.235, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000232 DAEEKQ010000000
VERSION     DAEEKQ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.235"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..154
                     /locus_tag="KLK57_003924"
     CDS             <1..154
                     /locus_tag="KLK57_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HBE0894169.1"
                     /translation="TAENIQIELRDDQDAALKNGDSKTVIVDEITRNAQFPLKARAIT
                     VNGNAS"
     gene            258..1172
                     /locus_tag="KLK57_003925"
     CDS             258..1172
                     /locus_tag="KLK57_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0894170.1"
                     /translation="MGKTISIKVLFGIYLLLMAGKVFAFSCNVDGGSSIGAGTTSVYV
                     NLDPVIQPGQNLVVDLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNN
                     VTYPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATL
                     GSGNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLS
                     FYLSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLG
                     LSATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1506..3785
                     /gene="ydeP"
                     /locus_tag="KLK57_003926"
     CDS             1506..3785
                     /gene="ydeP"
                     /locus_tag="KLK57_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HBE0894171.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKHSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAADRTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPHS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            4033..4230
                     /gene="safA"
                     /locus_tag="KLK57_003927"
     CDS             4033..4230
                     /gene="safA"
                     /locus_tag="KLK57_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HBE0894172.1"
                     /translation="MYATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     ISEMGRIARDLTHFIINSLQD"
     gene            4305..>5048
                     /gene="ydeO"
                     /locus_tag="KLK57_003928"
     CDS             4305..>5048
                     /gene="ydeO"
                     /locus_tag="KLK57_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HBE0894173.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQSHMGMNTGNTM"
BASE COUNT         1438 a         1085 c         1152 g         1373 t
ORIGIN      
        1 tactgccgaa aatattcaga tagagctgag ggatgaccag gatgctgcgt taaaaaatgg
       61 cgatagcaaa acggttattg ttgatgagat cactcgtaat gcacagtttc cacttaaggc
      121 aagagctatc acggtgaatg gaaacgcaag ctagggaacg atcgaggcgc taatcaatgt
      181 gatctacacc tggcaataac gtcgattcga ttttcttagc cttcttttta aatcaatttt
      241 taaccttagg atatatcatg ggtaaaacaa tcagtataaa ggtcctgttc ggtatttatc
      301 ttctattaat ggcaggcaaa gtttttgcct tcagttgtaa tgtcgatggc gggtcaagta
      361 ttggagcagg tacaacctct gtttatgtta atctcgaccc tgtaatacag ccgggccaga
      421 atctggttgt agacttgtct cagcatataa gttgctggaa tgattacggc ggctggtacg
      481 acactgatca tataaacctg gtacaaggct cagctttcgc aggctcccta cagtcatata
      541 aaggatcgct gtactggaat aatgtgacct accccttccc gttgacgaca aataccaacg
      601 tattggatat tggtgataag acaccgatgc cattaccgtt aaaactctat attaccccag
      661 ttggggcggc cggtggagtt gtcataaaag cgggggaagt cattgcccgt attcatatgt
      721 ataaaattgc aacattagga agcggaaatc cgcgtaattt tacgtggaat attatttcaa
      781 ataacagtgt tgttatgcca acaggcggct gcaccgttga tagtcgtaat gtcactgtgg
      841 atctcccgga ctttcctgga agcgcagaaa taccccttgg cgtatattgc tcaagcgaac
      901 aaaagctctc attttatctt tcgggtgcca ccactgacag tagccgacaa gtgtttgcta
      961 acacagcacc tgatgcaaca aaagcatcgg gagtgggtgt cactctaatg cgtaatggaa
     1021 aaatactggc tacgggagaa aatgtctccc tggggacagt aaataaaagc aaagtacctc
     1081 tgggtttaag cgcaacctat ggacaaacag gaaacaaggt ttcagccgga accgtccagt
     1141 cagtcattgg tgtcactttt atctatgaat aattgtgatt ttgaccttag tctatgcgct
     1201 ccattcacga aaattgcgca gataattatt ttgtgaaggc tattatccta cacctgtaag
     1261 aaaatccgcg cacaattgaa gccagagtct tattttttat attgaaaaat atgttaattt
     1321 aaacactatg agagatgatg cttaacttat cgtatttaat ctattgttta acgataatag
     1381 attaaaaata gaaagagagt agtcccctac tcttatatat ccatgttggc gataatccct
     1441 ttgtatgtac tgtgcatcat cgctattaca aatcctaata attcatttcc acacaggata
     1501 agtagatgaa gaaaaaaatt gaatcctacc agggtgctgc aggtggttgg ggtgctgtta
     1561 aatccgtagc gaatgcagta cgtaagcaga tggatatacg ccatgatgtt attgccatgt
     1621 ttgacatgaa taagccagag ggctttgact gtccgggttg tgcatggcca gatcctaagc
     1681 acagtgcgtc attcgacatt tgtgaaaacg gcgcaaaagc aatcgcctgg gaagtcacgg
     1741 ataagcaggt aaatgcctct ttctttgctg agaatacggt tcaatcatta cttacctggg
     1801 gagatcacga gcttgaggct gcggggcgtc tcactcagcc tttgaaatat gatgccgtca
     1861 gcgactgtta caagccatta agctggcaac aagctttcga cgaaattggc gcacgccttc
     1921 aaagctatag tgatcccaat caggttgaat tctatacttc gggccgcact tccaatgaag
     1981 ctgcctttct ttatcagctt tttgcccgtg aatacgggag caataacttt cccgactgct
     2041 ccaacatgtg ccatgaaccg acaagcgtgg gtttggcagc gagtatcggt gtaggtaaag
     2101 ggaccgtgtt gctggaagac tttgagaagt gcgatttagt catttgcatt gggcataacc
     2161 ctggtacaaa ccaccctcgc atgctgactt cgttgcgcgc tttagtgaaa cggggagcga
     2221 aaatgatcgc catcaatcct ctacaggaac gtggcctgga gcgatttacc gcaccgcaaa
     2281 acccgtttga aatgctgacg aactctgaga ctcagttggc cagtgcctac tacaacgtgc
     2341 gcattggtgg cgatatggcg ttgctcaagg ggatgatgcg cctgttaatt gagcgcgatg
     2401 atgctgcaag tgccgcaggt cggccctcat tgcttgatga cgaatttatt caaacgcata
     2461 ccgtcggctt tgacgagcta cgccgtgacg ttctcaattc cgagtggaaa gatatcgaac
     2521 gtatttctgg actaagtcag acacaaatcg ccgaactggc tgacgcatat gccgctgccg
     2581 atcgcaccat tatctgttac ggaatgggga tcactcagca cgaacatggt acccagaatg
     2641 tacagcaact ggtcaatctg ctgttgatga aaggtaacat tggcaagcct ggtgcgggta
     2701 tctgcccact acgaggtcac tctaatgtac agggcgaccg aaccgtcggt atcaccgaga
     2761 aaccgtctgc agagtttctg gctcgcctgg gtgagcgcta tggcttcacc ccacctcatg
     2821 cacctggaca tgctgcaatt gccagcatgc aagcaatatg cacggggcag gctcgagcat
     2881 tgatctgcat ggggggcaac tttgcactgg caatgccaga tcgggaagcg agcgctgtac
     2941 cgttaacgca attagatttg gcggtacacg tagccactaa gcttaaccgc tctcatctgc
     3001 tgaccgcacg gcatagctat attctgccgg ttctggggcg tagcgagatt gacatgcaaa
     3061 aaagcggtgc gcaggcggta accgttgagg attcaatgtc gatgattcat gcctcgcgtg
     3121 gcgtgttaaa acccgccggt gtaatgctga aatcagagtg tgcagtggtc gcgggaatcg
     3181 cgcaggcagc actaccccac agcgtggtag cctgggagta tctggtggaa gattatgatc
     3241 gcattcgcaa tgacattgaa gctgtgttac cagagttcgc cgactataac cagcgcatcc
     3301 gacatcctgg aggttttcac ctgataaatg cagctgctga aaggcgctgg atgacgccgt
     3361 caggtaaggc taatttcatt accagcaaag ggctgttaga agatccctct tcagcgttta
     3421 acagtaagct ggtcatggcg acagtacgta gccacgatca gtacaacacg acgatttatg
     3481 gtatggatga tcgctatcga ggggtattcg gtcaacgaga tgtggtcttt atgagtgcta
     3541 aacaagctaa aatttgccgt gtaaaaaacg gcgaaagagt taatcttatt gcgcttacgc
     3601 cagacggtaa gcgcagctca cgccgcatgg atagattaaa agtggtcatt taccctatgg
     3661 ctgaccgctc actggtgacc tattttccag aatcgaatca catgctaact cttgataacc
     3721 acgatccatt aagtggcatt cctggttata aaagtattcc ggttgaatta gaaccatcaa
     3781 attaattcgc ctcctcattt cttctgctgt catccgcaca gcagaagaat ttctcattga
     3841 ctattatcgc gcaatttgct cacattgatt aaattaaacc acacactata agatataaac
     3901 ctctgcctac agctgtaaga aactccgctc agtactgaag tgccagtcct atttcctctt
     3961 ttctccagcc tgttatatta agcatactga ttaacgattt ttaacgttat ccgctaaata
     4021 aacatatttg aaatgtatgc gaccacagtg aaaaacaaaa tcacgcaaag agacaactat
     4081 aaagaaatca tgtctgtaat tgtggttgtc ttattactga cccttacgtt gatagccatt
     4141 ttttcggcaa ttgatcagct gggtatttca gaaatgggtc gcattgcaag agatcttaca
     4201 catttcatta tcaatagttt gcaagactga aaataaacag caaattataa atatgaaatg
     4261 ttaaaaaagt atcgataaaa actttattgt tttaaggaga taaaatgtcg ctcgtttgtt
     4321 ctgttatatt tattcatcat gccttcaacg ctaacatttt agataaagat tacgccttct
     4381 ctgacggcga gatcctgatg gtagataacg ctgttcgtac gcattttgaa ccttatgagc
     4441 ggcattttaa agagatcgga tttactgaaa ataccattaa aaaatatcta caatgcacta
     4501 acatccagac agtgacggtg cctgttcctg cgaagttttt acgtgcttca aatgtaccga
     4561 ctggattgct taatgaaatg attgcttatc ttaactcgga agaacgcaat catcataatt
     4621 tttcagaact tttgcttttt tcttgcctgt ctatttttgc cgcatgcaaa ggtttcatta
     4681 cactattaac taacggtgtg ctatccgttt ccggaaaagt gagaaatatt gtcaacatga
     4741 agctggcgca cccatggaag ctgaaagata tttgtgactg cctgtacatc agtgaaagcc
     4801 tgttgaagaa aaaactaaag caagagcaaa cgacattctc acagattcta ttagatgcca
     4861 gaatgcagca cgcaaaaaat ttgatacgcg tagaaggttc agtcaataaa attgccgaac
     4921 aatgtggtta tgccagtaca tcttatttta tttatgcgtt ccgcaaacat ttcggcaaca
     4981 gtccgaagag agtttctaag gagtaccgtt gtcaaagtca catgggtatg aatacgggca
     5041 acacgatg
//
LOCUS       DAEEKQ010000233         5044 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.236, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000233 DAEEKQ010000000
VERSION     DAEEKQ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.236"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            73..1601
                     /gene="lsrA"
                     /locus_tag="KLK57_003929"
                     /pseudo
     CDS             73..1601
                     /gene="lsrA"
                     /locus_tag="KLK57_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
     gene            1595..1903
                     /locus_tag="KLK57_003930"
     CDS             1595..1903
                     /locus_tag="KLK57_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000911157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HBE0894174.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLMRKVVSAMISWIILSRARQKYGAASVVVDEQSWLSSGVKKSPY
                     "
     gene            complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="KLK57_003931"
     CDS             complement(2075..3523)
                     /gene="uxaB"
                     /locus_tag="KLK57_003931"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HBE0894175.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAISYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFAL
                     AALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDHVIGTQELVAIVLAEKDHWEQ
                     DLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(3750..4700)
                     /locus_tag="KLK57_003932"
     CDS             complement(3750..4700)
                     /locus_tag="KLK57_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HBE0894176.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGGFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPLTVMLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGL
                     VARMGGEEFAVAVPSVNPVDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRAS
                     YRTLTDDFNKLMVEADTCLYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(4839..>5044)
                     /locus_tag="KLK57_003933"
     CDS             complement(4839..>5044)
                     /locus_tag="KLK57_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4186 family protein"
                     /protein_id="HBE0894177.1"
                     /translation="ALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSL
                     SEEQQRYIVAVIYHWLVVQMNQP"
BASE COUNT         1315 a         1288 c         1230 g         1211 t
ORIGIN      
        1 taaggttatg aacaaattaa aagcagaaat acatttgttc aaaactcacc tgcaaaactg
       61 aacgggggaa atatgcaaac gagtgatacc cgcgcgttac cgctactttg cgcccgctcg
      121 gtttataaac agtattcagg ggtcaatgtc ctgtcgattt tacgttgcat caaggggagg
      181 tccacgccct gctcggcggc aatggtgccg gtaaatcgac gttaatgaag attattgccg
      241 gtattacccc tgctgatagc ggtacgctgg agattggggg caacaactac gtcagattaa
      301 cgccagttca tgctcatcag ctgggtattt atctcgttcc ccaggaaccg ctgcttttcc
      361 caagcctgtc gataaaagaa aacatcctgt ttgggctggc aaaaaaacag ctctccatgc
      421 agaaaatgaa gaacttgctg gcggcgctgg gctgctagtt tgatctgcat agtctggcag
      481 gatcgctgga tgtcgccgat cgccaaatgg tggaaatcct ccgcggcctg atgcgcgact
      541 cgcggattct gatcctcgat gaacctaccg cctcgcttac ccctgcggaa accgaacgct
      601 tgtttcgtcg cttgcaagag ctgcttgcta ctggcgtggg tattgttttt atctcgcata
      661 agctgccgga aattcgccag attgccgatc gaattagcgt gatgcgcgac ggaaccatcg
      721 ccttaagcgg caaaaccagc gaactgtcta ccgatgacat tattcaggcc atcacgccag
      781 tggtacggga aaaatcgctc tctgccagcc aaaaattatg gctggagtta cctggcaacc
      841 gcccacaaca tgccgccgga acgtcggtgc tgacactaga aaatctgacc ggcgaaggtt
      901 tcaggaatgt cagcctgacg ctcaatgccg gagaaattct gggtctggcc gggctggtgg
      961 gggccggacg cacagaactg gccgagacgc tctatggtct gcgtactttg cgtggcggac
     1021 gcattatgct gaatggtaaa gagatcaata aattatccac tggagaacgt ttactgcgcg
     1081 gtctggttta tctgccggaa gatcgccagt catccggact gaatctcgat gcttcgctgg
     1141 cctggaacgt ctgcgccctt actcataacc ttcgtggatt ctgggcgaaa accgcgaaag
     1201 ataatgccac cctggaacgt tatcgtcggg cgctgaatat taaatttaac caaccggaac
     1261 aagctgcacg gacattatcc ggtggcaacc agcaaaaaat cctgattgcc aaatgcctgg
     1321 aagcttcgcc gcaagcattg attgtcgatg agccgacgcg cggcgtggat gtctcggccc
     1381 gtaatgatat ctaccagctg ttgcgcagca tcgccgcaca gaatgtggca gtgctgctta
     1441 tctcctctga tctggaagag atcgaactga tggcagatcg tgtgtatgtg atgcatcagg
     1501 gcgaaattac ccactctgca ctgaccgggc gcgatattaa tgtcgagact attatgcgcg
     1561 ttgccttcgg cgatagtcag cgtcaggagg cgtcatgctg aagtttattc agaacaaccg
     1621 tgaaatcacg gcactgctgg cggtggtgct gctgtttgta ttacccggtt ttctcgaccg
     1681 ccagtattta agtgtgcaaa cgctgaccat ggtttatagc agcgcgcaaa tcctgatcct
     1741 gctggcaatg ggcgcgacgc tggtaatgct tatgcgcaaa gtagtaagcg cgatgatatc
     1801 ttggataata ctgagccgtg ccagacaaaa atacggcgca gccagtgttg ttgtggacga
     1861 acaatcatgg ttatcttcag gcgtgaaaaa gtcgccctat taaacgtgat tacgatcaca
     1921 ttctcaagac gctacttaca aattacctac aagcttacaa cattaacagg cgctgcatgt
     1981 ggcatctgtt aatgataaag gcacatagga tgttgtaact aatatagtga aataagaaac
     2041 ccggtcaaaa ccgggttcag aagtaacggt gttattagca caacggacgt acagcttcgc
     2101 gcatcccttt ttcaagaatc gcatccaggt cattagcaac ctgctcgacc agaccaggca
     2161 cttgcgtcag gtcctgctcc cagtggtctt tttctgccaa cacaatcgct accagttcct
     2221 gggtaccgat aacatggtca cgatgctggc tccagagttg ctgataacgt tccagccagt
     2281 gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc accacgatag aacgcaatca
     2341 atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc gtttgccttc tgccctgcca
     2401 gcagctgcgg caggatgcgg gtgcggaact tggtcatacc gttgagcgcg atagacagca
     2461 gctgatgctt atacgggtta cggaaacgcc cggtgactgc actggcgaaa gattccagtt
     2521 catcacgagg caaatccagt accggaataa tttcttcgta gatggctttt tcaacgaatg
     2581 cgcaaatttc agcatcgttc atcgcttcac ctacggtatc cagccctgcc tggaacgcca
     2641 ccggcaccag cgcggtgtgc gcaccgttga ggattgccac tttgcgttct ttatacggtt
     2701 taatatcgtc aacaatcagc acgttgagcg gatatttgtc caggcgcagc tcagtcgcta
     2761 aggatttcgg cccctgaatc acaaacaggt aaaagtgttc agcggtgtcg aggaaaccat
     2821 cgtgataacc cagttcttct tccagtttag ccacttcatc gcgcggataa ccggtaacga
     2881 tacggtccac cagcgtagaa cagaagctgt tagcctgatc cagccattga ataaatgctt
     2941 ctggcagtgc ccactcttgc gcatagcgca gcaccagttc acgcaacgcg tcgccattgt
     3001 agtcgatcaa ctcacacggg atgatgatcc aacctttatc cagcgcaccg ttgaaatggc
     3061 taaagcgttc gaacagcaga cgggtcagtt ttgccggata gcttatcgct ggcgcgtcat
     3121 cgaatttatc gcccgcatgg tagctgatcc ccgcttcggt ggtgttagag aaaacaaagc
     3181 gcatttccgg gttgtgcgcc agtttcagga attcatcgta ttcactgtag acgctgattt
     3241 cacgattaac cgagcgaatc agacgcgcgt cgctgaccgc ttcccccttc tcattcaggc
     3301 cacggataat ggtggtatac agaccatcct gcgtgctcag tgacggcggg aatgaagttt
     3361 caatcggacg aacaacgacc acgccagaat tcagatcggt gtgctcattc aggagatcga
     3421 tttgccagtc aacaaaggcg cgcaggaagt taccttcacc aaactgaatg atacgttctg
     3481 gatactgtgc accgggaaaa tcgcgacgat ttagtgtttt cacaatgggt tcccttctga
     3541 ttagtcatac aacctgtttt aattggtgcg acaggttagc aaactttaat acgccgaacc
     3601 cctgttttga tcaactcctg atgattaatg agcagtttta tgagaaaagt gtggcgcgga
     3661 tcatggttta atcgaggaaa tacgcctttt cctggatcat aaagtggtag aacacattgc
     3721 attcaaatcg cgcggaatga ataaagatgt cagacaactt cctcaccgta acgcatagtg
     3781 ctggtacggt tgcgcccatc tttcttcgag cgatacagac acgtatcggc ttcgaccatc
     3841 aatttattaa aatcatcggt cagcgttcgg tacgatgcgc gaccactacc gacgccaata
     3901 cttaccgtga gataaagcgt tttttgttgc caggtgaatg gttgcagttc aacgccttta
     3961 cggatttttt ccgccattag cagaccatcg acaggattca ccgacggcac tgcaacagca
     4021 aattcttcgc cgcccatgcg cgccaccagc cccttatcac cgacaatctt ctgaatatgc
     4081 cgggcaaaca cgcttaacac tttatcgcca cattcatgtc catagttatc gttaatgctt
     4141 ttgaaatagt cgatatcaag cagcatgacc gtcagaggca cgatcaacac gcccgttgag
     4201 aattgctcac tgaaccagtc cgcaagcaaa ggccagaaat ccagactatc aatactgacc
     4261 gaaccaatcg ccccgacaat agcgcataat aatgcggcaa tcagacagta attaaatagc
     4321 cgtaaggcac ttactggctc atacttattt ttccccagac gcttatcccg cacgaccagt
     4381 aaggcgacgg taacaataaa catcatattg gagaaattga taaccagtga aaccagtccc
     4441 cattcggtgg ttatggcatc ataaaccagc atcgccacat aactgatcgc atagtaatgc
     4501 agtcgattaa gccagacata gcgggcaaac ccccccgcca ttacgccatt cagcggccag
     4561 aaaagagaaa gttcgtgaac cagccgtaac tctgcaccaa taaaatagaa cagcgtggtt
     4621 agcgcaaaaa tagcgatgct attacgtagc agatgacctt cctggaacaa acggaaagtt
     4681 gaaatggggt gtacatgcat taattatctg tccatacaat tgcgggtggc aattatctca
     4741 acgtaataag taaattctca taaaggatag catgttcgct aacagcggcg gcaaataaag
     4801 gtaccctggg aatgctattt tatcgttttc cgcaattctc atggctgatt catctgtaca
     4861 accagccagt gatagattac cgccacgata taacgttgtt gttcttcgct taacgaaaca
     4921 ccctgcggaa tgttatgcca cttagccaga caaccgcgac agcaggtcgc ggtggcgtgc
     4981 tgagcgataa acaccggatg cccgcgcatg ggggtttgct taccgtcatt agccggtaat
     5041 gccg
//
LOCUS       DAEEKQ010000234         5042 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.237, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000234 DAEEKQ010000000
VERSION     DAEEKQ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.237"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            82..197
                     /gene="rrf"
                     /locus_tag="KLK57_003934"
     rRNA            82..197
                     /gene="rrf"
                     /locus_tag="KLK57_003934"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(315..758)
                     /locus_tag="KLK57_003935"
     CDS             complement(315..758)
                     /locus_tag="KLK57_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HBE0894178.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEVLVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVEE"
     gene            915..1844
                     /gene="metA"
                     /locus_tag="KLK57_003936"
     CDS             915..1844
                     /gene="metA"
                     /locus_tag="KLK57_003936"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HBE0894179.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            2113..3714
                     /gene="aceB"
                     /locus_tag="KLK57_003937"
                     /pseudo
     CDS             2113..3714
                     /gene="aceB"
                     /locus_tag="KLK57_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            3744..5035
                     /gene="aceA"
                     /locus_tag="KLK57_003938"
                     /pseudo
     CDS             3744..5035
                     /gene="aceA"
                     /locus_tag="KLK57_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
BASE COUNT         1260 a         1320 c         1288 g         1174 t
ORIGIN      
        1 tgttttggcg gatgagagaa gattttcagc ctgatacaga ttaaatcaga acgcagaagc
       61 ggtctgataa aacagaattt gcctggcggc cgtagcgcgg tggtcccacc tgaccccatg
      121 ccgaactcag aagtgaaacg ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga
      181 gtagggaact gccaggcatc aaattaagca gaaggccatc ctgacggatg gcctttttgc
      241 attggcgcag aaaaaaatgc cgggtgcgac gctggcgcgt cttctccagc ctgggcaggc
      301 acggcagggg cagcttattc ctccacatac gccagattca gcaacggata cggtttcccc
      361 aaatcgtcca cctcagagcg tcccgtaacc ttaaaaccca ccttcttata gaacccaacc
      421 gcctgctcat tttgctcatt aacgttggtt gtcagttccg gtgccatcga gagcgcatgc
      481 tccaccagca tccgacccac gccgcagccg cacacatcag gatcgataaa cagcgcatcc
      541 atatgctgcc cacttaacaa cataaaccca accggctgat cccgttcatt aaccgcgacc
      601 cacaacggcg cttccggcag gaaggaacga actagcacct ccagctcggc ccgatactct
      661 gctgataaaa aatcgtgtgt ggcatcgaca gaacgacacc aaatcgcaac gagttcctcc
      721 ccttcctcat gccgtgagcg gcgaatacta ataaccattt tctctccttt tcgtcattct
      781 tatattctaa cgtagccttt tccttgaaac tttctcacct tcaacatgca ggctcgacat
      841 tggcaaattt tctggttatc ttcagctatc tggatgtcta aacgtataag cgtatgtagt
      901 gaggtaatca ggttatgccg attcgtgtgc cggacgagct acccgccgtc aatttcttgc
      961 gtgaagaaaa cgtctttgtg atgacaactt ctcgtgcgtc tggtcaggaa attcgtccac
     1021 ttaaggttct gatccttaac ctgatgccga agaagattga aactgaaaat cagtttctgc
     1081 gcctgctttc aaactcacct ttgcaggtcg atattcagct gttgcgcatc gattcccgtg
     1141 aatcgcgcaa cacgcccgca gagcatctga acaacttcta ctgtaacttt gaagatattc
     1201 aggaacaaaa ctttgacggt ctgatagtta ccggcgcgcc gctgggcctg gtggagttta
     1261 atgatgtcgc ttactggccg cagatcaaac aggtgctgga gtggtcgaaa gatcacgtca
     1321 cctcgacgct gtttgtctgc tgggcggtac aggccgcgct caatatcctc tacggcattc
     1381 ctaagcaaac tcgcaccgac aaactctctg gcgtttacga gcatcatatt ctccatcctc
     1441 atgcgcttct gacgcgtggc tttgatgatt cattcctggc accgcattcg cgctatgctg
     1501 actttccggc agcgttgatt cgtgattaca ccgatctgga aattctggca gagacggaag
     1561 aaggggatgc atatctgttt gccagtaaag ataagcgcat tgcctttgtg acgggccatc
     1621 ccgaatatga tgcgcaaacg ctggcgcagg aatatttccg cgatgtggaa gccggactag
     1681 acccggatgt accgtataac tatttcccgc acaatgatcc gcaaaacaaa ccgcgcgcga
     1741 gctggcgtag tcacggtaat ttgctgttta ccaactggct caactattac gtctaccaga
     1801 tcacgccata cgatctacgg cacatgaatc caacgctgga ttaatcttct gtgatagtcg
     1861 atcgttaagc gattcagcac cttacctcag gcaccttcgg gtgccttttt tatttccgaa
     1921 acgcacctca gcaagtgaat aaattttatt catattgtta tcaataagtt atcaagtatt
     1981 tttaattaaa atggaaattg tttttgattt tgcattttaa atgagtagtc ttagttgtgc
     2041 tgaacgaaaa gcgcacaacg atccttcgtt cacagtgggg aagttttcgg atccatgacg
     2101 aggagctgca cgatgactga acaggcaaca acaaccgatg aactggcttt cacaaggccg
     2161 tatggcgagc aggagaagca aattcttact gccgaagcgg tagaatttct gactgagctg
     2221 gtgacgcatt ttacgccaca acgcaataaa cttctggcag cgcgcattca gcagcagcaa
     2281 gatattgata acggaacgtt gcctgatttt atttcggaaa cagcttccat tcgcgatgct
     2341 gattggaaaa ttcgcgggat tcctgcggac ttagaagacc gccgcgtaga gataactggc
     2401 ccggtagagc gcaagatggt gatcaacgcg ctcaacgcca atgtgaaagt ctttatggcc
     2461 gatttcgaag attcactggc cccggactgg aacaaagtga tcgacgggca aattaacctg
     2521 cgtgatgcag ttaacggcac catcagctat accaatgaag caggcaaaat ttatcagctc
     2581 aagcccaatc cagcggtttt gatttgtcgg gtacgcggtc tacacttgcc ggaaaaacat
     2641 gtcacctggc gtggtgaggc aatccccggt agcctgtttg attttgcgct ctatttcttc
     2701 cacaactatc aggcactgtt ggcaaagggc agcggtccct atttctatct gccgaaaacc
     2761 cagtcctggc aggaagcggc ctggtggagc gaagtcttca gctatgcaga agatcgcttt
     2821 aatctgccgc gcggcgccat caaggcgacg ttgctgattg aaacgctgcc cgccgtgttc
     2881 cagatggacg aaatccttca cgcgctgcgc gaccatattg ttggtctgaa ctgcggtcgc
     2941 tgggattaca tcttcagcta tatcaaaacg ttgaaaaact atcccgatcg tgtcctgcca
     3001 gacagacagg ccgtgacgat ggataaacca ttcctgaatg cttactcacg cctgctgatt
     3061 aaaacctgcc ataaacgcgg tgcttttgcg atgggcggca tggcagcgtt tattccgagc
     3121 aaagatgaag agcgtaataa ccaggtgctc aacaaagtaa aagcggataa agcgctggaa
     3181 gtcaataacg gtcacgatgg cacatggatc gctcacccag gccttgcgga cacggcaatg
     3241 gcggtattca acgacattct cggctcccgt aaaaatcagc ttgaagtatt gcgcgaacaa
     3301 gacgcgccga ttactgccga tcagctgctg gcaccttgtg acggtgaacg caccgaagaa
     3361 ggtatgcgcg ccaacattcg cgtggctgtg cagtacatcg aagcgtggat ctccggcaac
     3421 ggctgcgtgc cgatttatgg cctgatggaa gatgcggcga cggctgaaat ttcccgtacc
     3481 tcgatctggc agtggatcca tcatcaaaaa acgttgagca atggcaaacc ggtgaccaaa
     3541 gccttgttcc gctagatgct gggcgaagag atgaaagtca ttgccagcga actgggcgaa
     3601 gaacgtttct cccagggccg ttttgacgat gccgcacgct tgatggaaca gatcaccact
     3661 tccgatgagt taattgattt cctgaccctg ccaggctacc gcctgttagc gtaaaccacc
     3721 acataactat ggagcatctg cacatgaaaa cccgtacaca acaaattgaa gaattacaga
     3781 aagagtggac tcaaccgcgt tgggaaggca ttactcgccc atacagtgcg gaagatgtgg
     3841 tgaaattacg cggttcagtc aatcctgaat gcacgctggc gcaactgggc gcagcgaaaa
     3901 tgtggcgtct gctgcacggt gagtcgaaaa aaggctacat caacagcctc ggtgcactga
     3961 ctggcggtca ggcgctgcaa caggcgaaag cgggtattga agcagtctat ctgtcgggat
     4021 ggcaggtagc ggcggacgct aacctggcgg ccagcatgta tccggatcag tcgctctatc
     4081 cggcaaactc ggtgccagct gtggtggagc ggatcaacaa caccttccgt cgtgccgatc
     4141 agatccaatg gtccgcgggc attgagccgg gcgatccgcg ctatgtcgat tacttcctgc
     4201 cgatcgttgc cgatgcggaa gccggttttg gcggtgtcct gaatgccttt gaactgatga
     4261 aagcgatgat tgaagccggt gcagcggcag ttcacttcga agatcagctg gcgtcagtga
     4321 agaaatgcgg tcacatgggc ggcaaagttt tggtgccaac tcaagaagct attcagaaac
     4381 tggtcgcggc gcgtctggca gctgacgtga cgggcgttcc aaccctactg gttgctcgta
     4441 ccgatgccga tgcagcggat ctgatcacct ccgattgcga cccgtatgac agcgaattta
     4501 ttaccggcga gcgtaccagt gaaggcttct tccgtactca tgcgggcatt gagcaagcga
     4561 tcagccgtgg cctggcgtat gcgccatatg ctgacctggt ctggtgtgaa acctccacgc
     4621 cggatctgga actggcgcgt cgctttgcac aagctatcca cgcgaaatat ccgggcaaac
     4681 tgctggctta taactgctcg ccgtcgttca actggcagaa aaacctcgac gacaaaacta
     4741 ttgccagctt ccagcagcag ctgtcggata tgggctacaa gttccagttc atcaccctgg
     4801 caggtatcca cagcatgtgg ttcaacatgt ttgacctggc aaacgcctat gcctagggcg
     4861 agggtatgaa gcactacgtt gagaaagtgc agcagccgga atttgccgct ataccttcgt
     4921 atctcaccag caggaagtgg gtacaggtta cttcgataaa gtgacgacta ttattcaggg
     4981 cggcacgtct tcagtcaccg cgctgaccgg ctccactgaa gaatcgcagt tctaagcaac
     5041 cg
//
LOCUS       DAEEKQ010000235         5005 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.238, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000235 DAEEKQ010000000
VERSION     DAEEKQ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.238"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            12..754
                     /locus_tag="KLK57_003939"
                     /pseudo
     CDS             12..754
                     /locus_tag="KLK57_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308314.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            complement(796..1248)
                     /locus_tag="KLK57_003940"
                     /pseudo
     CDS             complement(796..1248)
                     /locus_tag="KLK57_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1312..1458
                     /locus_tag="KLK57_003941"
                     /pseudo
     CDS             1312..1458
                     /locus_tag="KLK57_003941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1458..2615)
                     /gene="intS"
                     /locus_tag="KLK57_003942"
     CDS             complement(1458..2615)
                     /gene="intS"
                     /locus_tag="KLK57_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HBE0894180.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIVPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDEKVE"
     gene            complement(2777..2851)
                     /locus_tag="KLK57_003943"
     tRNA            complement(2777..2851)
                     /locus_tag="KLK57_003943"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2816..2818),aa:Arg,seq:cct)
     gene            complement(2927..3859)
                     /locus_tag="KLK57_003944"
     CDS             complement(2927..3859)
                     /locus_tag="KLK57_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HBE0894181.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            4153..4908
                     /gene="mlaA"
                     /locus_tag="KLK57_003945"
     CDS             4153..4908
                     /gene="mlaA"
                     /locus_tag="KLK57_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HBE0894182.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT         1216 a         1165 c         1170 g         1454 t
ORIGIN      
        1 gggtggatga tatggcaact acatgttcag ttatattgat tttggagtcc tttgatgttt
       61 atttcggaaa agagagtgtg tttctggaga gaggttcatc tgtacttgtc gactctagct
      121 ctagagattt tttcctgaca tatcctgaaa gagtgatagt ggcggatttt ggcgctgagt
      181 ttattagtcg ctatttgaaa gctaataact taagggatat ttctgattgt agggaatatc
      241 tatcttattt aaaaataaac tttgctgact tcagtttaat taaaggatta attagttggg
      301 ctaatcactg tgctgaatac atagaaattt ttgatgagtc tattgctttt acatgtctct
      361 ctgcattttc ttctgaaaaa caatttggag tatttctgtt tggatgtttg aaaagcacag
      421 ggctaaagtt aaaacgatta ttcatacgga tttatctgca ccatggcgtc ttaaggatat
      481 atcatcaaga ttatatctca gcgaaagttt actaaagatg aaattgaaag aagagggggt
      541 atcattcagt aagatcatac ttgatgagag gatgcaaatg gctgaatatt tactcagcac
      601 tcgttgttat cctattagta aagtagctaa ggtctgtggt tatgccagtg tctcatactt
      661 tacttatgta tttagacgtt attttggtgt ttctccaagt caatactctc agaggagttc
      721 agaaagtaaa attcttactc accagggaat ctgatcattg ttcttgcccc cttatttcca
      781 gacagggggt gtatcttaag ttaacgttac ccgctgacgt cgatattctc gcggagagcg
      841 ataacccagc gcactgtgcg gatggttttc attgtaatgt tcgatcgcca ctgcaagatt
      901 atgcaatgcc gttcttacat tcggtttcgg catgaacgcg atgtagtctt ccttcatcgt
      961 tttcacgaac ctttctgcca ttccattact ctgaggactg ctgattgccg ttgtgcaggg
     1021 ctccagattc aactctctgg cgaactgccg tgtttcatgc gctctatacg ctgaaccgtt
     1081 atctgtcagc cactggatgg actgttccgg tactttatcg ccaaagcgtt tttctatcgc
     1141 tcctaacatg acatcctgca ctgtcgcttt atcgtaacct cccgtgcttg cggcccagtc
     1201 tatggcttca cgatcgcaac agtccagggc gaacgtaaca cgcagttttg aatcgccacg
     1261 ggtttaacag acacctcaga gtcatttaag atggcttaaa gagaggtgcc catgagcggt
     1321 aagcgttatc ccgaagagtt taaaactgaa gcagtcaaac aggttgttga tcgcggttat
     1381 tctgttgcca gcgttgcaac acgtctcgat atcactaccc acagccttta tgcctggata
     1441 aagaagtacg gtccggatta ctccactttt tcatcaagcc aatccgccca ccactgcatc
     1501 atttctctgc gcttatcgag atactgagca tggttgtaaa taccgcgcac agatccgccg
     1561 ttggcatgtg ccagctgcac ttcaatagcg tcagcaggcc attcgtgctc gttcataatc
     1621 gtgctgaatt catgcctgaa tccgtgaccg ctttccagac cttcatagcc gatttgtttg
     1681 atcacaagca ataccgcgtt ctcgcagatt ggcttcttct tatcgttgcg cccggcaaaa
     1741 acaaactctg agactggttt ggtgattgag cttagcgtag tgagaagttc aaccacctgg
     1801 tctgacatcg ggaccacatg aattttgcgt cccttcatca cactggcgtc gatggtgata
     1861 atcctgtttt caaaatcgac gttcttccat agcatggaac gaagctcttt tgttcttagg
     1921 gctgtgtagc gtaaaacttt ggtcgcaatg agcgatacga tacttcctga aaatgttgcc
     1981 agtgctttgt tgaatgcagg catctggtct gctggaagga acgggaagtt cttcttgcgg
     2041 tatcctttca tggcgtctgc aaggtcaggt gccgggttat atttagccct tccggtgaca
     2101 atagcgtaac gaaaaacctc gccgcatctt ctgcgggctt tgttggctcg ctccattgca
     2161 ccgcgatctt caaatctgcg gattacttcc agcagttgca tcggctcaat atcctgaatc
     2221 tcaagaccgc cgatgatggg taaaatgtcg tcatcaaaca ttttggcaag ttcagttgca
     2281 tagcctactg accagacttg cttcttgtgc tcgtaccatt ccttgtaaat cgcactaaat
     2341 gaattgttgt tagacgaagc ctttttcgcc tttaccggaa cgatgccaac cgagatgtct
     2401 ttcctcgcag tccatgcctt atcccttgct tcctgcaaag tcataagcgg atattttccg
     2461 acggtcagga ttttctcctt accgtcaatc ttgtagcgaa gctgccatac ctttttcccg
     2521 gatacaggaa cataaaggta caggccatta ccatcgagaa ggcggtatgg tttttctttc
     2581 ggctttgctg cttcaatctg cttaacggtg agcatgggta aaaatccggt gggtaaaatt
     2641 attttatcca ctttttaccc gtcatggagt gcggctgtca acgatctgac gcgaaccatg
     2701 acgaaccgtg aatatacgga aggcttgata ttcaggggat tttgcggact ggtacggatg
     2761 ggagcgaact gataaatggt gtcccctgca ggaatcgaac ctgcaattag cccttaggag
     2821 gggctcgtta tatccattta actaagagga caatgcggca tgagtatacc cgctaatgga
     2881 gtgcggggta agtacgctgc cgctcgattg cttaaaccct cgccatttat gccgggtttt
     2941 tatcattttt cttaatgttt tccgcacgtt ctgctttttg gcgtgcttct gctttacgct
     3001 tgttgctcat gtcgttacga atctgtgcat gactcattaa cgcgaagata aaggtgccgc
     3061 cgcagatgtt ccccgctaaa gtaggtagtg cgaagggcca gatgaaatcg ctccagtgca
     3121 gcgtaccgtt aaacaccaga tagaggattt caacagaacc gaccacgata tgggtggtgt
     3181 cacctagggc aataagccag gtcatcaata taatcaccac aatctttgcc gcacctgctg
     3241 caggaaacat ccataccata gtggcgatca gccagccgga aatgatcgcg ttggcaaaca
     3301 tctcgctggg ggtgttcttc atcacatcca tgccgatttt gacaaatgca tcgcgagttt
     3361 cttcattgaa gataggcata tattcaaatg cccacgcagc aataccagtc ccgagaatat
     3421 tacccagcag cacgactccc cataaccgca taagtaagcc aacgttgctc attgtcggtt
     3481 tttgcatgac gggtagtacc gcagtcacgg tgttttcggt aaataattgc tggcgggcca
     3541 ttatgacgat aataaaacca aaggtataac cgagattctc cagtaagaag ctgcccggca
     3601 caccttccag ttcgacatga aatatccctt ttgccagtaa cgaagcgccc atcgacagac
     3661 ccgccgcaat ggctgaccac agtagcgcca ttgcgtcgcg ttccagctct ttttcaccat
     3721 cctggcggat atgctcatga attgccatcg cccgggaggg gagtcggtct tcatctattt
     3781 ctattttttt gccgcgctct ttttcttcgc tctcaacttc aatttcgtcg ctgtgttgat
     3841 caattttgtc gttgtccatg gtctcttctc ttgaattagc acgtatagct aaagcgtagc
     3901 ggcttttttg ctcgcaactg gcgggagtta ctctgaaaat gtagaaaagg ctgcgtttgc
     3961 ctttttctgt ttctatagaa tcaagtagcc tacagggcgg cgattaccag gctatgatca
     4021 aatcagcaaa tcagggcgtc tggacatcag ttgacgtgct gttacaatcg cccacaccta
     4081 aacaggcgga tacggtatcg ttccgtcatg gatggcaaac tgcataagcc ataaaaaaac
     4141 agggagacat ttatgaagct tcgcctgtcg gcgcttgctc tgggaactac gcttctggtg
     4201 gggtgtgcga gttccggtac agatcagcaa gggcgttctg acccgttaga agggttcaac
     4261 cgcaccatgt acaacttcaa cttcaacgta ttagacccgt atattgttcg accggtcgct
     4321 gtcgcctggc gtgattatgt tccgcaaccg gcgcgtaatg gtttgagcaa ctttactggc
     4381 aaccttgaag aacctgcggt gatggttaac tacttcttgc agggcgaccc ttatcagggg
     4441 atggtccact ttacccgctt tttcctgaac accattttgg ggatgggcgg ctttattgat
     4501 gttgcaggga tggcgaaccc gaaactgcaa cggactgaac ctcaccgctt cggtagtacg
     4561 cttggtcatt atggcgtggg ttatgggcct tacgttcagt taccgttcta cggtagcttc
     4621 acgctgcgtg atgacggtgg tgatatggcg gatggtcttt acccggttct ttcctggctg
     4681 acctggccga tgtctgtggg taaatggacg cttgaaggga tcgaaacccg cgctcagctg
     4741 ctggattccg atggtctgct gcgtcagtcg tccgatcctt atattatggt gcgcgaagcg
     4801 tacttccagc gtcatgattt catcgctaat ggcggcgaac tcaaaccgca ggaaaatccg
     4861 aacgcacaag cgattcagga tgatttaaaa gatattgatt ctgaataaga aacaaataaa
     4921 aaaggtgagt cgcaaaactc accttttttg ttgttatccc tcaattacca caagtaaaag
     4981 gagataaacc gacaatgacc cggac
//
LOCUS       DAEEKQ010000236         4936 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.239, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000236 DAEEKQ010000000
VERSION     DAEEKQ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4936
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.239"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(148..3369)
                     /gene="carB"
                     /locus_tag="KLK57_003946"
     CDS             complement(148..3369)
                     /gene="carB"
                     /locus_tag="KLK57_003946"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HBE0894183.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(3387..4535)
                     /gene="carA"
                     /locus_tag="KLK57_003947"
     CDS             complement(3387..4535)
                     /gene="carA"
                     /locus_tag="KLK57_003947"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HBE0894184.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         1128 a         1366 c         1256 g         1186 t
ORIGIN      
        1 agtgtgagct aaatcctgct tatgtcatca aaaatatcga ttccccaaaa gatttaatta
       61 atgggcaaaa aaaacggtcc tcatcagaga accgtattac ttattcgatc aaattagcgg
      121 atgaaaaata tccgccatga cacgctatta tttgatctgc gcgtgcattt cctgcaccga
      181 aattactttt tcggtcgcgt cggcattcag cgccatcgcg gtagcgaaac caccgttcag
      241 ggtggtgtca taatgcactt tatattgcag cgcactgcga cggatcacgc gggagtcttc
      301 aatcgcgcga cggcctgaag tggtgttgat gatgtaggta tattcgccat tcttgatgcg
      361 gtcctgaatg tgcggacggc cttcatgcac cttgttcacc agacgcggat taatgcccgc
      421 ttcgcccagc acaatcgccg tgccgtgggt cgcatccagc tcgaagccct gtttcagcag
      481 ttttgccgcc aggtccacca cgcgttcttt atcgccttcg cgcacggaaa gcaacgcacg
      541 accgtgtttc ttcatggtgg agttgctgcc cagctgcgct ttggcaaacg cttcagcgaa
      601 ggtgcggccc acgcccatca cttccccggt ggagcgcatt tctggcccta acagcgggtc
      661 aacacccggg aatttattga acggcagcac cacttctttc accgagtagt acggcgggat
      721 aacttctttg gttacgccct gctcagccag cgatttgcca gccatcacac gcgccgccac
      781 ttttgccagc ggtacgccgg tggctttgga gacgaacgga acggtacgcg ccgcacgcgg
      841 gttaacttca atcaggtaga cttcgttgtt tttcaccgca aactgcacgt tcatcagacc
      901 gcgcacctgc aattcgaagg ccagtttctg cacctgctgg cgcatcacat cctgaatttc
      961 ctgacttaag gtgtaggctg gcagagaaca tgcggagtca ccggagtgca cgcccgcctg
     1021 ctcgatgtgc tccatgatgc cgccaatcag caccatttcg ccgtcgcaga tggcatccac
     1081 gtcaacttct accgcgtcat caaggaagtg atccagcaac actggcgcat cgttagacac
     1141 gctgaccgcc gtctggaagt agcgacgcag gtcagcttcg tcatagacga tttccatcgc
     1201 ccgaccgccg agaacgtaag acggacgtac caccagtggg tagccaatct ctttcgcctt
     1261 ctctaccgcc atttcaatag cggtaacggt ggcgttcgcc ggttgtttca gtttcagacg
     1321 ctcaaccgca tgctggaagc gttcacggtc ttctgcacgg tcgatagcat ccgggctggt
     1381 gccgataacc ggtacgccag cagcttccag cgcgcgcgcc agtttcagcg gggtctgacc
     1441 gccgtactga acgataacgc ctttcggctt ctcaatacgc acgatttcca gcacatcttc
     1501 cagagttacc ggctcgaagt agaggcgatc ggaagtgtcg tagtcggtgg agacggtttc
     1561 cgggttacag ttaaccataa tggtttcgta accgtcttcg cgcagcgcca gcgaggcgtg
     1621 tacacagcag tagtcgaatt cgataccctg accgatacgg ttcgggccgc cgccgagaac
     1681 catgattttt tcacggtcgg tagacggatt cgcttcgcac tcttcttcat aagtggagta
     1741 catgtaagcg gtgtcggtgg cgaactctgc cgcacaggta tccacgcgct tataaaccgg
     1801 gtgcaggtca tactggtcgc gcagcttgcg gatttccgct tcgcgcacgc ccgccagttt
     1861 tgccagacgc gcatcggcaa agcctttgcg tttcagctgg cgcaggaatt cagcgttcag
     1921 gccagtgatg cccacttccg ctactttctc ttccagacgc accagttctt caatctgtac
     1981 caggaaccag cggtcaatat tggtcaggtt gaacacgccg tccacagaca ggcccgcacg
     2041 gaaggcatcg gcgatgtacc agatacgctc tgcgccagca tctttcagtt cgcgacggat
     2101 tttggttaac gcttccgggt catcgaggct cactttcggg tcgaatccag tcgcaccgac
     2161 ttccaggccg cgcagcgctt tttgcaggga ttcctgctgc gtgcgaccaa tcgccatcac
     2221 ttcgccaacc gatttcatct gagtggtcag acggtcgtta gcaccagcga atttttcgaa
     2281 gttgaagcga ggaattttag taaccacata gtcgatggac ggctcgaagg aggccggagt
     2341 acgtccgcca gtgatgtcgt tcatcagttc gtcgagggtg taacccaccg ccagtttcgc
     2401 cgccacttta gcaatcggga aaccggtcgc tttcgacgcc agcgccgaag aacgggacac
     2461 gcgtgggttc atttcgataa caatcaggcg accgtttttc gggttcaccg caaactggac
     2521 attggaacca ccggtttcaa cgccgatttc acgcagcacc gccatcgagg cgttacgcat
     2581 gatttgatat tctttgtcgg tcagcgtttg ggctggcgcg acagtgatgg agtcaccggt
     2641 atggatgccc atcgcatcaa agttttcgat agagcagacg atgatgcagt tgtcgttttt
     2701 atcacgcacc acttccatct cgtactcttt ccagccgatc agcgactcat caatcagcag
     2761 ctctttggtt ggggagagat ccagaccgcg ggcgcaaatt tcttcaaact cttcgcggtt
     2821 ataagcgata ccgccgccgc taccgcccat ggtaaaggat gggcgaataa tgcacgggaa
     2881 gcccacgtca gcggcaaccg ccagcgcttc ttccatcgtg tgtgcgatac cggaacgcgc
     2941 ggtttccaga ccaattttct tcatcgctac gtcgaaacga cggcggtctt ctgctttatc
     3001 aatcgcatcg gcggtcgcac caatcatagt cacgccgaac tcttccaaca cgccctgacg
     3061 ctccagctcc agcgcgcagt tcagcgccgt ctgaccgccc atcgttggca gcaccgcgtc
     3121 cgggcgctct ttttcaataa tcttgcgtac tacttcccag tgaatcggct cgatgtaggt
     3181 cgcatcggcc atttccgggt cggtcatgat ggtcgccggg ttggagttca ccagaatgac
     3241 gcggtaaccc tcttcgcgca gggctttaca tgcttgcgcg ccagagtagt caaactcaca
     3301 cgcctgaccg ataacaatcg ggcccgcacc cagaatcagg atacttttta tatctgtacg
     3361 ttttggcatg gctcttttac tcctgattac ttagcggttt tacggtactg ctcaattaac
     3421 tcgataaagt ggtcgaacaa cggcgctgcg tcgtgtggcc cagggctggc ttccgggtga
     3481 ccctggaagc tgaacgccgg tttatcagtg cgatgaatgc cctgtaacgt accgtcgaac
     3541 agggatttat gcgtgacacg caggtttgca ggtaatgttg cttcatccac cgcaaaaccg
     3601 tggttctggg cggtgatcat caccacgttt ttctccacat ctttaaccgg atggttgccg
     3661 ccgtggtgac caaatttcat tttgacagtc ttcgcaccgc tcgccagcgc cagcagctga
     3721 tgaccgaggc agatgccgaa taccggaata tcggtttcga ggaatttctg gatggcggta
     3781 atggcgtaat cgcacggggc cgggtcgcca ggaccgttgg agaggaagat gccgtctgga
     3841 ttcattttca gcacatcttc cgcagaagtt tgcgccggaa cgatggtcag gcgacagcct
     3901 ctatccacca gcatccgcag gatgttgcgc ttggcaccaa aatcataagc cacgacgtgg
     3961 aacggcagct cgtcttcttt tttcgcttct ggcaggccgc cggtcaacgt ccagctccct
     4021 tgtgtccagc tataggcttc tgcggtggtc acttcttttg ccagatccat gccattcaga
     4081 cctgggaacg cgcgggcttt ttctaacgcc aacgccgcat ccgggttatc gcctgcgata
     4141 atgcagccat tctgtgcgcc tttctcgcgc agtaaacgcg tcagcttacg ggtatcgata
     4201 tcggcaatcg ccacgatgtt atggcgcttc aggtaagaag agaggtcttc ggtattacgg
     4261 aagttgctgg caatcagcgg cagatcgcga atcaccagac cttgtgcatg tacctgagaa
     4321 gattcttcat cggcgtcatt agtgccgaca ttgccaatat ggggataagt aagagtaacg
     4381 atttgacgag aataggaagg atcagtgagg atttcttgat aaccggtcat tgaagtattg
     4441 aaaacgactt ccccaaccgc cgaacctgtt gcccctatgg cccgaccgtg aaactgggtt
     4501 ccgtcttcca gaaccaatag cgctgactta atcaaaacac cctccagaga atattcactc
     4561 actttatttg catattaatt cacaatgatg acgtaaatca atgcaaatct gcttaccgac
     4621 gaatttctgg caaacggcgg cattctggag atatgggcgc taaaagtcaa cttaatggcg
     4681 gtatttttta tattattttg tggctcatgc atattttaca ggcattaagc gacaaaaaga
     4741 tcatataacc tgttctggaa aacgcttgcg cagcaagaag attgcatgaa ataaaaacga
     4801 gcaggaaaaa gtggaccaaa tggtcaaaat ttacattaaa acaacaaaaa tagaagacaa
     4861 aacatgtaat ttttggcact tatagaatat ttaaaaacat aacactaaaa ataaaagggc
     4921 caaaaattaa agccct
//
LOCUS       DAEEKQ010000237         4799 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.240, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000237 DAEEKQ010000000
VERSION     DAEEKQ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4799
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.240"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            241..582
                     /gene="raiA"
                     /locus_tag="KLK57_003948"
     CDS             241..582
                     /gene="raiA"
                     /locus_tag="KLK57_003948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HBE0894185.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    685..810
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            686..733
                     /gene="pheL"
                     /locus_tag="KLK57_003949"
     CDS             686..733
                     /gene="pheL"
                     /locus_tag="KLK57_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HBE0894186.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            833..1993
                     /gene="pheA"
                     /locus_tag="KLK57_003950"
     CDS             833..1993
                     /gene="pheA"
                     /locus_tag="KLK57_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HBE0894187.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINMSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEIFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="KLK57_003951"
     CDS             complement(2036..3157)
                     /gene="tyrA"
                     /locus_tag="KLK57_003951"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HBE0894188.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="KLK57_003952"
     CDS             complement(3168..4238)
                     /gene="aroF"
                     /locus_tag="KLK57_003952"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HBE0894189.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4447..>4799
                     /locus_tag="KLK57_003953"
     CDS             4447..>4799
                     /locus_tag="KLK57_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HBE0894190.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVESASQ
                     LHEVWQKGADENAST"
BASE COUNT         1311 a         1229 c         1096 g         1163 t
ORIGIN      
        1 tgagctgccg tttttttatt ctgttagttg tgaaactgaa gcgatttagt cactatcgat
       61 ctcatcaaat atggctcgct ttgagatatt cctcaagtaa aaaaacacct cttcctgcga
      121 tttctcacaa aaaagattcg ttgacaaaaa gtgacaaaat tatgagattc ccatcacaca
      181 ttttgacatc aggaacggta tgctgaattc accaagacgg gaagacaaga ggtaaaattt
      241 atgacaatga acattaccag caaacaaatg gaaattactc cggccatccg ccaacatgtc
      301 gcagaccgtc tcgccaaact ggaaaaatgg caaacacatc tgattaatcc acatatcatt
      361 ctgtccaaag agccacaagg gtttgttgct gacgccacaa tcaatacacc taacggcgtt
      421 ctggttgcca gtggtaaaca tgaagatatg tacactgcaa ttaacgaatt gatcaacaag
      481 ctggaacggc agctcaataa actgcagcac aaaggcgaag cacgtcgtgc cgcaacatcg
      541 gtgaaagacg ccaacttcgt cgaagaagtt gaagaagagt agtcctttat attgagtgga
      601 tcgccaacgc gccttcgggc gcgttttttg ttgacagcgt gaaaacagta cgggtactgt
      661 actaaagtca cttaaggaaa caaacatgaa acacacaccg tttttcttcg cattcttttt
      721 taccttcccc tgaatgggag gcgtttcgtc gtgtgaaaca gaatgcgaag acgaacaata
      781 aggcctccca aaccgggggg ccttttttat tgataacaaa aaaggcaaca ctatgacatc
      841 ggaaaatcca ttactggcgc tgcgagagaa aatcagcgcg ctggatgaaa aattattagc
      901 attactggca gagcggcgcg aactggccgt cgaggtggga aaagccaaac tgctctcgca
      961 tcgcccggta cgagatattg atcgtgaacg cgatttactg gaaagattaa ttacgctcgg
     1021 taaagcgcac catctggacg cccattacat tactcgcctg ttccagctca tcattgaaga
     1081 ttccgtatta actcagcagg ctttgctcca gcaacatctc aataaaatta atccgcactc
     1141 agcacgcatc gcttttctcg gccccaaagg ttcttattcc catcttgcgg cgcgccagta
     1201 tgctgcccgt cactttgagc aattcattga aagtggctgc gccaaatttg ccgatatttt
     1261 taatcaggtg gaaaccggcc aggccgacta tgccgtcgta ccgattgaaa ataccagctc
     1321 cggtgccata aacgacgttt acgatctgct gcaacatacc agcttgtcga ttgttggcga
     1381 gatgacgtta actatcgacc attgtttgtt ggtctccggc acgactgatt tatccaccat
     1441 taatacggtc tacagccatc cgcagccatt ccagcaatgc agcaaattcc ttaatcgtta
     1501 tcctcactgg aagattgaat ataccgaaag tacgtctgcg gcaatggaaa aggttgcaca
     1561 ggcaaaatca ccgcatgttg ctgcgttggg aagcgaagct ggcggcactt tgtacggttt
     1621 gcaggtactg gagcgtattg aagcgaatca gcaacaaaac ttcacccgat ttgtggtgtt
     1681 ggcacgtaaa gccattaaca tgtctgacca ggttccggcg aaaacgacgt tgttaatggc
     1741 gaccgggcaa caagccggtg cgctggttga agcgttgctg gtgctgcgca accacaatct
     1801 gattatgacc cgtctggaat cacgcccgat tcacggtaat ccatgggaag agatatttta
     1861 tctggatatt caggccaatc ttgaatcagc ggaaatgcaa aaagcattga aagagttagg
     1921 ggaaattacc cgttcaatga aggtattggg ctgttaccca agtgagaacg tagtgcctgt
     1981 tgatccaacc tgatgaaaag gtgccggatg atgtgaatca tccggcactg gattattact
     2041 ggcggttgtc attcgcctga cgcaataaca cgcggctttc actctgaaaa cgctgtgcgt
     2101 aatcgccgaa ccagtgctcc accttgcgga aactgtcaat aaacgcctgc ttatcgccct
     2161 gctccagcaa ctcaatcgcc tcgccgaaac gcttatagta acgtttgatt aacgccagat
     2221 tacgctctga cgacataata atgtcggcat aaagctgcgg atcctgagca aacagtcgcc
     2281 cgaccatcgc cagctcaagg cggtaaatcg gcgaagagag cgccagaagt tgctcaagct
     2341 gaacattttc ttctgccaga tgcagcccat aagcaaaagt agcaaagtgg cgcagagcct
     2401 gaataaacgc catattctga tcgtgctcga cagcgctaat acgatgcagc cgagcgcccc
     2461 agacctgaat ttgctccaga aaccattggt atgcttccgg cttacgtcca tcacaccaga
     2521 ccacaacttg ctttgccagg ctaccgctgt ccgggccgaa catcgggtgt aaccccagta
     2581 ccgggccatc gtgcgccgcc agcatggcct gtaatggtcc atttttcact gatgccagat
     2641 caaccagaat acaatctttc ggtaaaggcg gtaatttgcc gataacttgc tcagtaacgt
     2701 ggattggcac actaacaatc accattccgg catcggcaac aatatcagcc gctcgatccc
     2761 agtcatgttg ctccagaatc cgcacctgat aacccgagag tgtcagcatc ttctcgaaca
     2821 ggcgtcccat ctgaccgcca ccgccgacga taaccaccgg acgcagtgac ggacaaagtg
     2881 ttttaaatcc tttgtcgttt tcactggagt aagattcacg catcacccga cgcaaaacat
     2941 cctcaatcag atctggcggt acacccagag cttccgcctc tgcacgacgc gaggccaaca
     3001 tagatgcctc gcgctccgga acataaatag gcagtccaaa gcggcttttc acctcgccca
     3061 cttcagcaac cagttccaga cgcttcgcta ataaattcag cagcgcttta tcgacttcat
     3121 caatttgatc gcgtaatgcg gtcaattcag caaccataat aaacctctta agccacgcga
     3181 gccgtcagct gcccgttcag atcctgatga atttcacgca gcaaggcatc ggtcatttcc
     3241 cagctaatgc aggcatcggt tacggataca ccgtatttca tttcactgcg cggttgctca
     3301 gaagactgat tgccctcgtg gatattactt tcgatcatca gaccaataat tgagcgattg
     3361 ccatctttga tttgagcaac cacggattct gccaccgcag gctgacggcg ataatcttta
     3421 ttggaattac cgtggctgca atctaccatc agagacgggc gcagtcccgc ctgttccatc
     3481 tctttttcac attgcgcaac atccgcaggg ctatagttcg gcgctttacc accgcgcagg
     3541 atgacatgcc cgtccggatt cccctgagtt tgtagcaacg caacctgccc tgcctggtta
     3601 atgccaacaa aacggtgcgg ctgggcggcg gcgcgcatag cgttaattgc tgttgccaga
     3661 ctgccgtcgg tgccgttttt aaaaccaacc ggcatggaaa gcccggaggc catttcacgg
     3721 tgagtttgcg attccgttgt acgagcacca attgctgacc agctaaacag atcgcccagg
     3781 tattgcgggc tattcggatc taacgcttcc gtcgccagtg gcagtcccat attcaccagc
     3841 tcaagcagca atttacgcgc gatctgcagc ccggcttcta catcaaaaga gccatccata
     3901 tggggatcgt taattaaccc tttccagccg acagtggtac ggggtttttc aaaatagacg
     3961 cgcattacca gatagaggct atcgctgacc tctgcggcaa gggctttaaa tcgacgagca
     4021 tattccagag cagtttccgg atcatgaatg gaacaaggac cacaaactac cagcagacga
     4081 ggatcgcgcc cggcgataat atctgaaatg gttttacgcg agtcagcaat ctgggcttct
     4141 tgttgcaggc tcaatggaaa agcggccttc agttgttccg gagtcattaa aacctgttcg
     4201 tcggtaatat gtacgttatt cagcgcgtct ttttgcatga tggcgatcct gtttatgctc
     4261 gtttgcgata gttgatcctc agcgaggatg acgtaacgat aacacataaa gtaaagtttt
     4321 caatccatat ttcgtacatt tttctttaca cgaacaattt gctcgcgctt ttgatgctta
     4381 tttcgtacaa ataaatttac acctccttta aaaacaatcc gctatgcttt gaaaaaggag
     4441 aaagaaatga tgaaaaagtt tatcgccccc ttattggctt tactggttag cggatgtcag
     4501 attgatcctt atactcacgc gccaaccttg accagcaccg actggtatga tgtcggtatg
     4561 gaagatgcga tatcgggcag cgccataaaa gatgacgatg catttagcga ttcacaggcg
     4621 gatcgcggtc tataccttaa aggatatgcc gaaggacaaa agaaaacttg ccagaccgat
     4681 tttacttatg cccggggact ttccggtaaa agctttcctg ccagctgtaa caatgttgaa
     4741 agtgccagcc aactgcatga agtatggcaa aaaggggctg atgaaaatgc cagcacaat
//
LOCUS       DAEEKQ010000238         4796 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.241, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000238 DAEEKQ010000000
VERSION     DAEEKQ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.241"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            6..81
                     /locus_tag="KLK57_003954"
     tRNA            6..81
                     /locus_tag="KLK57_003954"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:39..41,aa:Thr,seq:ggt)
     gene            121..236
                     /gene="rrf"
                     /locus_tag="KLK57_003955"
     rRNA            121..236
                     /gene="rrf"
                     /locus_tag="KLK57_003955"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(299..1057)
                     /locus_tag="KLK57_003956"
     CDS             complement(299..1057)
                     /locus_tag="KLK57_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HBE0894191.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQLGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTKAFLSQVIH"
     gene            complement(1065..2168)
                     /locus_tag="KLK57_003957"
     CDS             complement(1065..2168)
                     /locus_tag="KLK57_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HBE0894192.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2178..3359)
                     /locus_tag="KLK57_003958"
                     /pseudo
     CDS             complement(2178..3359)
                     /locus_tag="KLK57_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
     gene            complement(3427..4452)
                     /locus_tag="KLK57_003959"
     CDS             complement(3427..4452)
                     /locus_tag="KLK57_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0894193.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTDYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPVVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
BASE COUNT         1315 a         1238 c         1146 g         1097 t
ORIGIN      
        1 agcgtgctga tatggctcag ttggtagagc gcacccttgg taagggtgag gtccccagtt
       61 cgactctggg tatcagcacc actttttagg ttaaagttcg gcagattaga aaagaatttg
      121 tctggcggca gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
      181 cgtagcgccg atggtagtgt agggtctccc catgcgagag tagggaactg ccagacatca
      241 aatcaagcga aaggccatcc gaaaggatgg cctttttgct tttcgaacta acattcaatt
      301 aatggattac ctgcgataaa aatgccttcg tacgctctga tttaggatgc gcaaaaaatt
      361 catcaggtgc agcttgctcc actatttccc cacgatccat aaaaatcacc cggtcagcga
      421 cggttcgcgc aaaccccatc tcatgcgtta cacacaacat tgtcataccc gattgcgcca
      481 gcccaatcat cgtatccagc acctctttca ccatctccgg atcgagcgcc gacgttggct
      541 catcaaacaa cataattttc ggcttcatac acagcgaacg cgcaatggca acgcgttgct
      601 gctgaccacc agaaatttgt ccgggaaact tatgcgcatg ttcggcaatt ctcacccgct
      661 caaggtaatg catcgccaga gcttcagcct ctttctttgg catcttgcgt acccaaatcg
      721 gtgccagggt acagttctgt agaacggtca gatgagggaa gagattgaaa tgctgaaaga
      781 ccattcccac ttcctgcctg acgcgctcaa tattgcggat atcttcatta agttcgatgc
      841 catctactac gatccgtccc tgttgatgtt cttccagatg attaatacaa cgaatggttg
      901 tcgatttacc ggaaccagaa gggccacaca gaacgatccg ttctcccagt tgcacggtta
      961 aatttatatt tttcaaaaca tggaattgtc cataccattt attgacgttt tccagcgtaa
     1021 tcatcgcgtt agcaggttgc agtaaaattt ggctcatagt gtcctcaatg cggtgtacgc
     1081 ccggtgttaa aacgtttttc cagatgctgg ctatagcgcg acatgctgaa acagaagatc
     1141 cagtagatca gtgcggcgaa aacataccct tccgtcgaca tacccaacca ggcgggatca
     1201 acggttgcct gctgaacgct actgaaaaga tcgaacaacc cgataatgat caccaggctg
     1261 gtatctttga agagtgcgat gatggtattt accagcccag gaattaccag cttcaacgcc
     1321 tgtggcagaa taaccagccc ctgagttttc cagtaaccca atgccagtga ctctgccgct
     1381 tcatattgcc ctttaggcag cgcctgtaat ccgcctcgca cgacttccgc aacatatgct
     1441 gactgaaaca ggatcacgcc aaccagcgcg cggatcagtt tgtcgatatt ggtgccttct
     1501 gccataaaca aaggcagcat gaccgaagac ataaacaata cggtaatcag cggtacgccg
     1561 cgccagaact cgataaaaat gaccgataag atacgcacaa tcggcatatg ggagcggcga
     1621 cctaacgcca gtaagatccc ccacggtagc gcccccgcaa tcccaacgga tgcaataatt
     1681 aaagttagcg tcagcccgcc ccattgccgg gtttcaaccc gctccagacc aaaaaaaccg
     1741 ccatacatca gccaccagac aatcagtggg taaatcaccg cccagaccgc aatatagcga
     1801 ccgcgatgcg ggagtatttt ccagaacatt ggtgcgattg acacaaggcc aatcagtaat
     1861 gccaggttaa ttcgccagcg ttggtcgtgt gggtaaagtc catacataaa ctgaccaaat
     1921 cgttcgtgga tgaagaccca acaggcaccg gcttttgtgc agtcggcacg cgttgagcca
     1981 acccaattag cctgcaaaaa tgcccagttc agcaacggtg ggatcagttc ccacatcaac
     2041 catatgcagc caatagtcag caggctattg ctccagctgg agaacagatt ttttcgcacc
     2101 cacaccatcg cgcggcttga gttatggctc gctgggcgcg agggctgaga cagcaatact
     2161 tttgtcatca tgactcctta gcgttcaacg atcgcgatgc ggcggttata gatattcatc
     2221 agcagcgaga tagtcaggct gataatcaga tagaccgaca tggtcatggc tatcgtctcg
     2281 atggcttgcc ccgtctggtt cagcacggtg ccagcaaaca gcgaaaccat atcgggatag
     2341 ccaatagcgg cggcaagaga ggagtttttg acgatgttga gatactggct ggttaacggc
     2401 ggaataatca ctcgcagtgc ctggggaata atgacctagc gtagcgtaac cgggttgggt
     2461 aatcccagtg accgagccgc ttcatgttga ccataaggca ctgcctggat cccagcgcgg
     2521 ataatctcgg cgataaatgc agatgtataa accgaaagtg ccagcgttaa ggctgccagt
     2581 tcaggaatta aaaccatccc gccgcggaag ttaaagcctc gtagggctgg gacatcccag
     2641 tgtagtgctg cgccaaaaag ccattgcgcc agtaaaggca aaccaatgat caacactgcg
     2701 gcgatcggcc aggtgcggcg cagttgtccg gtctttatct ggtatgtttt attaaagcgg
     2761 aatagcccaa cagaaaggac tatagccata acaacagcca ggataaacgc aataaatccg
     2821 tctcccagct gcggtgacgg aatataaagc ccacgattgc tcaaaaaggc cagatcaaac
     2881 gcgctcacag cctggcgtgg tccgggcaaa ttgcgcaaca cggcaaagta ccagaagaag
     2941 atttgcagca gcgggggaat attacggaag atctcaatat aaattgtgga aagctttcgt
     3001 agcagccagt tatccgaaag tctcgccaga ccgataaaga agcccaaaac agaagcgaac
     3061 acaatacaca atgcagaaac cagtagcgta ttgagtaagc cgacaataaa aacgcgtccg
     3121 taggtgtcgc cctgctggta atcgatcaaa tgctggacaa taccgaagcc agcgccgcga
     3181 tccagaaagg caaaacctga agtaatgcca cgattattga gattcgttac agtgttgtga
     3241 aataaccaac caacaatgcc gacaacagca acaacggcaa ggatctggaa taaccaggca
     3301 cgaaccgtag ggttggcaaa agagagtgag cctttaacgg ttgagcggcg atgagacata
     3361 gaaacctcgg aaagcataac gctgaagaga gcgccgccgt agcgacgctc gttacctcac
     3421 accgacttaa cgcacgggcg gcgcgtactg aataccgccg ttattccaga gattattttg
     3481 cccacgttta attttcagcg ggctttctga acctacgtta cgctcgaaaa tttccgagta
     3541 gttacccacc tgtttgatga tgttataggc ccatttatta tccagcttca gatccttgcc
     3601 gtaatcgcct tctttaccca gcagatgtgc catatcaggc gttgctggat tagccgcttt
     3661 ttcatcgacg ttctgggaat tgatgcccat ctcttcagca ttcagcatgg cgaaaagcgt
     3721 ccagcgtaca atcgagaacc attcatcatc gccacgacga actaccggcc caagcggttc
     3781 tttagagata acctccggta agacaatcca ttcagctggg ttgcttaatt tgatgcgcag
     3841 ggcatacagt tgcgactgat ccgaggccag cgtatcgcag cgaccagatt ccagtgcctt
     3901 cgctgattcg tcagagcgat cgaaagtcac cggtgtgtac ttcatattgt ttgccttgaa
     3961 atagtcggcg acgttgagtt cggtatctgt acccgcctga atacagacgg tagcgccatc
     4021 cagttctttc gcgcttttta gccccgcttt atcgtgcgtc aggaagccaa tgccgtcgta
     4081 atcagtgacg ccagtaaatg ccattcccat cccggcatcg cgagatgaag tccaggtcgt
     4141 attacgggag agcaaatcca cctcccctga ctgtaaagcg gtgaagcgtt cttttgcagt
     4201 gagcggggta tatttcactt tcgtgtcgtc accaaataca gcagcggcaa caccacgaca
     4261 aacatctaca tcaatacctg aaaacttacc gtctgcatcg gcataagaga acccaggtaa
     4321 tccatcactg atcccgcatt gcacaaaacc ttttttctgt acggcatcaa gcgtcgcgcc
     4381 agcatgcgcc tgatttgcaa cggcaagcag cacgctggcg gcagccagtg tggctatcat
     4441 catctttttc ataatgcttc ctgtgaggcg aaaattatcg ttataggacg tttgggaaac
     4501 gtcttttacc tgtctgtggc catcctctta taagcaaaag tagtgccatc ttttgcgcac
     4561 gatttcgcgt cgcaataagc tgagtgtaga caggaaataa taaaaacagc gccccgcaat
     4621 ggtgcgaaaa tgaaacatgc gccacatttt ggcgcaaaat atttacgcgt gttttactaa
     4681 gcagaataat gattgttagc gtgagtatat ttctggtggg aggtgtgaaa gttaaattga
     4741 tgcgttattt cgaaagcagc gaaataacgc gatataacat gctaaggtaa tgactc
//
LOCUS       DAEEKQ010000239         4741 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.242, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000239 DAEEKQ010000000
VERSION     DAEEKQ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.242"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..737)
                     /locus_tag="KLK57_003960"
     CDS             complement(<1..737)
                     /locus_tag="KLK57_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HBE0894194.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQID"
     gene            complement(878..2911)
                     /gene="metG"
                     /locus_tag="KLK57_003961"
     CDS             complement(878..2911)
                     /gene="metG"
                     /locus_tag="KLK57_003961"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HBE0894195.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRSDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            3043..4152
                     /gene="apbC"
                     /locus_tag="KLK57_003962"
     CDS             3043..4152
                     /gene="apbC"
                     /locus_tag="KLK57_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HBE0894196.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLNALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            4397..4678
                     /locus_tag="KLK57_003963"
     CDS             4397..4678
                     /locus_tag="KLK57_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HBE0894197.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLAINGRISPPTCSMAMV
                     NGQPQPHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1147 a         1177 c         1183 g         1234 t
ORIGIN      
        1 atatcaatct gttgaaggtg tattattaca tcagtagcat attccagacc gccttcctgg
       61 acgatctgat ccataacatc ctcacgcact ttatatcttg tgcatttcat ttcaagtgcc
      121 gtgagtacag ccattactgg tgatggtaac tgtgttgttt tcaggccgtc gatctgactg
      181 atcgcctggg cgacggtgtt ttgccattct aaagaacagg cgctgaagtc aaatgtggct
      241 gttttattat cccgttgcga cacactacag gctaagcgac gtaattgggt caatttgtct
      301 gcttgctgaa caggaactcc gggaaagcgg cgatgactta acgtggtgtg tacaacttcg
      361 ggcggaaaaa cgacagcaat atctttcgcc agccacggta aaacagcatc tgtatttttg
      421 tcaggcgcac gggtttctgc tacgaaagag ggttgttcct gaataatggt ggataagtcc
      481 gctaccttga gagagcccgt taccgtttgg gaaggttgca aagaattatc cttcgcttgc
      541 tccacatacc ccttcttcac cttctccgca atcagcttaa gctccgcttt tgccgctgcc
      601 gcagcatctg aaaaactttt tatctggctt tgccctttgg tgccaacttt tccccaactg
      661 atatgcaact cgtttccctg ttgctcaact gcccagaatt tatgtgattt ttcgtcctga
      721 tagataaagt gtctcattct gcatccctgt aattttatcc agttgaatgc agatgctacc
      781 agtatttatg cgggttagag agagacaaaa tcgcaagaaa acaactttag gaatttataa
      841 agaaaaggcg ctgtcgatgc agcgccttga agggggatta tttcacctga tggcctggtt
      901 tagcaccagc atccgggctt aacaggaaga tatctttccc gccaggacca gcagccatta
      961 ccatgccttc ggagatacca aagcgcattt tacgcggtgc caggttggcc accataatgg
     1021 tgtgacgccc gatcagtgcc tgcggatccg ggtaggcaga acgaataccg gagaagacat
     1081 tgcgtttttc accgccgaga tccagtgtca ggcgcagcag tttgtcagaa ccttcaacaa
     1141 actctgcgtt ttcgatcagc gccacgcgca ggtcaacttt tgcgaagtcg tcaaaggtga
     1201 tggtttcctg aatcggatca tctgccagtg ggccagtaac tggcgcggca gctgctttca
     1261 cttcttcttt agacgcttcc accagtgctt caacctgctt catatcgatg cggttataca
     1321 gcgctttgaa cggattcact ttgtggccca gcagcggttg ctggatacca tcccaggtca
     1381 gttctgtatt gaggaatgct tctgcgcgct cggtcagttt cggcagtacc ggcttcaggt
     1441 aagtcatcag cacgcggaac aggtttatgc ccattgagca aattgcctgc aggtcggcat
     1501 cgcggccttc ctgttttgcc accacccacg gagcctgctc gtcgacatag cggttagcca
     1561 gatcagccag cgccatgatt tcgcgcactg ctttaccaaa ttcgcggctt tcccacgctt
     1621 caccaatcac ttcagcggca tcagtgaagg ttttgtacaa ctgcgggtca gccagttcgc
     1681 ttgccagcac gccgtcaaaa cgcttgttga taaagcccgc attacgagag gccaggttaa
     1741 ccactttgtt cacgatatcg gcattcacac gctgaacgaa atcctccagg ttgagatcga
     1801 tatcatcaat gcgcgaagag agtttcgcag tgtagtagta acgcaggctg tcagcgtcaa
     1861 aatgattcag ccaggtgctg gctttaataa aggtgccgcg agacttggac atctttgcgc
     1921 cgttcaccgt cacatagcca tgaacaaaca ggttggtcgg cttgcggaag ttgctgcctt
     1981 ccagcatggc aggccagaac aggctgtgga agtaaacaat atctttaccg atgaagtggt
     2041 acagctcggc ggtggagtct ttcttccagt attcatcgaa gcttacgctg tcgctgcgct
     2101 tgtcgcacag attcttgaaa gaacccatgt agccaatcgg tgcgtccagc cagacgtaga
     2161 aatatttgcc cggcgcgttc ggaatttcaa aaccaaagta tggcgcgtcg cgggagatat
     2221 cccactgttg caggccggat tcaaaccact cctgcatttt atttgccacc tgctcctgca
     2281 acgcaccgct gcgggtccat gcctgcaaca tttcgctgaa agagggcaga tcaaagaaga
     2341 agtgttcaga atcacgcatt accggcgtag cgccagaaac caccgatttc ggctcgatca
     2401 attcagtcgg gctgtaggtc gcgccgcaga cttcgcagtt atcgccgtat tgatccgggg
     2461 atttacattt cgggcaggtg cctttcacaa aacggtccgg caggaacatg cctttttccg
     2521 gatcgtacag ctgagagatg gtgcggtttt taataaaacc gttctctttc aggcgagagt
     2581 agataagttc tgacaactgg cggttctctt cgctgtgcgt cgagtgatag ttgtcatagc
     2641 tgatgttaaa gcctgcgaaa tcagtctgat gctcctgact catttcgcca atcatctgct
     2701 ccggggtgat accaagctgc tgagctttca gcatgatcgg tgtaccgtgg gcatcgtcgg
     2761 cgcagatgaa gttgacctcg tggccgcgca ttcgctggta acggacccag acatcagcct
     2821 ggatgtgctc cagcatatgg ccgaggtgga ttgagccgtt agcgtacggc agtgcgcacg
     2881 tcaccagaat tttcttcgcg acttgagtca tagtaggcat tacttcttaa tagtgaaaag
     2941 gggttagata gtaccaaatg ggaaaatgtt aagtaagtac ttctctatat tcttcaggta
     3001 aactatcagc tgatggttta tctatacaaa aaaggagtcg ggatgaacga acaatcccag
     3061 gccaaatcac cagaagcctt gcgcgcaatg gtcgccggga cactggccaa ttttcagcac
     3121 ccaaccctga agcataacct caccacgctg aacgcgttgc accatgtcgc ctggatggac
     3181 gacacgttgc atgtggaact ggttatgcca ttcgtctggc atagcgcgtt tgaagagtta
     3241 aaagagcaat gtagcgccga actgctgcgt atcaccgggg cgaaagctat cgactggaaa
     3301 ctgtcgcaca atatcgccac cttaaaacgc gtgaagaatc agccgggtat taatggtgtg
     3361 aagaacatca ttgcggtcag ttccggtaaa ggcggcgtgg gtaaatcctc cacggcggta
     3421 aacctggcgc tggcactggc ggcggaaggg gcgaaagtcg gcattctgga cgccgatatc
     3481 tacggcccat caattcccac catgctgggc gcagaaaatc agcgtccaac ttcgccggac
     3541 ggtacccata tggcaccgat tatgtctcat ggcctggcaa ccaattcaat tggttatctg
     3601 gtcactgacg acaatgcgat ggtgtggcgt ggaccgatgg cgagcaaggc gttaatgcag
     3661 atgttgcagg aaaccttgtg gccggatctc gactatctgg tgcttgatat gccgccaggc
     3721 accggtgaca ttcaattgac gctggcgcaa aacattccag taactggtgc agtcgtggtc
     3781 accaccccgc aggacatcgc tttgatcgat gcgaaaaaag gcattgtgat gttcgagaaa
     3841 gtcgaggttc cggtgcttgg tattgtcgag aacatgagtg tgcatatttg cagtaactgt
     3901 ggtcaccatg aaccgatttt cggtaccggt ggtgcagaga aactggctga aaaataccac
     3961 acacagctgc tggggcagat gccgttacac atctccttac gtgaggatct cgataaaggc
     4021 acgccaacgg ttatcagccg tccggagagc gaatttaccg ctatctaccg ccagctggct
     4081 gaccgcgttg cagctcagct ctactggcag ggtgaagtca ttccaggcga gatttccttc
     4141 cgcgcggtct aacgcctatt aaccattctg gttattcttc atacaccgca gagatgttac
     4201 attgatgcgg tgtattagtt tttgccgcat taatgaacac ttatttaata ctgcgaatat
     4261 tctgcggacc ttcgtccgaa actctacaaa gtcgattgat catacaggca atgcttcatt
     4321 atcatccttc gtggctgtgt agtggatgta cagctatagt ttgttatgca ctcattctat
     4381 ccaggcataa ccttcaatga ataagtattg gctgtcagga attatttttc ttgcctatgg
     4441 attggcttct cccgcattct cttcagagac tgccacatta gcgattaatg gcaggatcag
     4501 tcccccaaca tgcagtatgg ctatggttaa tggtcaacct caaccgcatt gtggtcagtt
     4561 aacgtataat gttgacaccc gtcatctgtt ttcttcgccc gtcaaaggtg taacgacaga
     4621 agtggttgtt gcaggtagcg atagcaaacg tcgaatcgtg ttaaatcgct acgattaatt
     4681 tggcaacctg atttttattg atatcaacaa taccatttac ataaatctac attaattgat
     4741 a
//
LOCUS       DAEEKQ010000240         4722 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.243, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000240 DAEEKQ010000000
VERSION     DAEEKQ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4722)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4722)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4722
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.243"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            105..1079
                     /gene="hemB"
                     /locus_tag="KLK57_003964"
     CDS             105..1079
                     /gene="hemB"
                     /locus_tag="KLK57_003964"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HBE0894198.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1185..2036)
                     /gene="tauD"
                     /locus_tag="KLK57_003965"
     CDS             complement(1185..2036)
                     /gene="tauD"
                     /locus_tag="KLK57_003965"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HBE0894199.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRYQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2033..2860)
                     /gene="tauC"
                     /locus_tag="KLK57_003966"
     CDS             complement(2033..2860)
                     /gene="tauC"
                     /locus_tag="KLK57_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HBE0894200.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     VLAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2857..3624)
                     /gene="tauB"
                     /locus_tag="KLK57_003967"
     CDS             complement(2857..3624)
                     /gene="tauB"
                     /locus_tag="KLK57_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HBE0894201.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3637..4599)
                     /gene="tauA"
                     /locus_tag="KLK57_003968"
     CDS             complement(3637..4599)
                     /gene="tauA"
                     /locus_tag="KLK57_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0894202.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTAELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1075 a         1381 c         1220 g         1046 t
ORIGIN      
        1 ctctttcatt ctcttcttgt caacatcacg acaactttcg taaaacactc ctgccccgct
       61 tcaggtatac tgtgcacctt aattccacaa acatcaggca gaccatgaca gacttaatcc
      121 aacgccctcg tcgcctacgc aaatctcctg cgctgcgcgc tatgtttgaa gagacaacac
      181 ttagccttaa cgacctggtg ttgccgatct ttgttgaaga agaaattgac gactacaaag
      241 ccgttgaagc catgccaggc gtgatgcgca ttccagagaa acatctggca cgcgaaattg
      301 aacgcatcgc caacgccggt atccgttccg taatgacttt tggcatctct caccataccg
      361 atgaaaccgg cagcgacgcc tggcgggaag atggactggt ggcgcgtatg tcgcgcatct
      421 gcaagcagac tgtgccagaa atgatcgtca tgtcagacac ttgtttctgt gaatacactt
      481 ctcacggtca ctgcggtgtg ctgtgcgagc atggcgtcga caacgacgcg actctggaaa
      541 atttaggcaa gcaagccgtg gttgcagctg ctgcaggcgc agacttcatc gccccttccg
      601 ccgcgatgga cggccaggta caggcgattc gtcaggcgct ggacgctgca ggctttaaag
      661 atacggcgat tatgtcgtat tcgaccaagt tcgcctcttc cttttatggc ccgttccgtg
      721 aagctgccgg aagcgcatta aaaggcgacc gcaaaagcta tcagatgaac ccaatgaacc
      781 gtcgcgaggc gattcgtgaa tcactgctgg atgaagccca gggcgcagac tgcctgatgg
      841 ttaaacctgc tggagcgtac ctcgacatcg tgcgtgagct gcgtgaacgt actgaattgc
      901 cgattggcgc gtatcaggtg agcggtgagt acgcgatgat taagttcgcc gcgctggcgg
      961 gtgctataga tgaagagaaa gtcgtgctcg aaagcttagg ttcaattaag cgtgcgggtg
     1021 cggatctgat tttcagctac tttgcgctgg atttggctga gaagaagatt ctgcgttaat
     1081 ttttttcctc tcagaaaggg ggagttaacc tgttgaaagt acggtgaacg ccccctcacc
     1141 ctaaccctct ccccaagggg gcgagggaac cgtccgcact cgtatcaccc cgcccgataa
     1201 aacggtttat ccccaaggat cgtcgcccga tgcataatcc gccgctgtgg caggtaatcg
     1261 gcattggcat agtgctgagt cacgcggtta tcccaaatcg caatatcatt cggttgccag
     1321 cgccaacgca cctgaaactc cggtttagtg atatgggcga ataaaaagcc taacaaggct
     1381 tcgctctctt tctcgctgac atcgacaatt cgcgtagtaa agccttcatt cacaaacagc
     1441 gcctgtttac cgctcaccgg atgcgttcgc accaccggat gtagcaacgg aggatttttc
     1501 gcgaccgcct cgcgccagcg ttgatgttcc tcctcggttt tgcggtattt gtattccggg
     1561 aacgatttac ggaaatcatg ctccgcacgc agcccgctca gcagctggcg gaagggaaca
     1621 gagagcgcct cataggccgc aataccgctg gtccagagcg tatcaccgcc ggtcgaaggt
     1681 aactctttag ctgccagaat cgcccctgcg ggtggcgttt caataaatgt cacatcggta
     1741 tgccagttgt cgttatccgg cggattatcg ttatgggtat ccagcacgat gatctcgtca
     1801 accccttcgg catgagggta aacagggtga atatgcagtt cgccaaaacg ctgggccagc
     1861 gcgcgttgct gctgcggcgt aatagcttga tcgcgcagaa ataccacctg atagcgcagc
     1921 accgcatggt aaagctgttc aaactgatta tcgcttaacg ggcgcgtcag gtcggcaccc
     1981 gaaatttgtg cgccgatata cggccccagc ggggtaatac tcagacgttc actcattgta
     2041 cttctccatg ccagggcgtc agacggcgct gtaacgcgcg cagacccagt tctaaaagaa
     2101 aggcgataat cgcaatcacc gcgatccccg ccagtaccac gtcagtcgcg agaaattcac
     2161 ccgctgactg aaccataaat cctaaaccgc gtgtcgcggc aatcagctcc gccgctacca
     2221 gcgtagacca gcccacaccc agaccaatgc gtaatccggt gaggatttcc ggcagcgcgc
     2281 cgggcaaaat gacaaaccac agcacctgcg cacggctggc acccagcgac tgggcggcac
     2341 gaatgcgaac ctgctgcgcg cttttcaccc ccgccagcac cgacatcgcc accggtgcaa
     2401 aaatcgctaa atagatcaat aagatcttcg aggtttcacc aataccaaac cagatcacca
     2461 tcagcggcaa ataagccagc ggcggcaccg gacgataaag ttcgattacc ggatccagaa
     2521 tgccgcgcac cgtcgggctt agccccatgg cgatcccgac cggaataccg atcaccaccg
     2581 ccgcgagtag cgccagcaca atgcgcgtca ggctggctgc cagatgctgc cacagcgtgg
     2641 cgtccataaa tccctgcggt ccggcaatgg tgagcagttt tgccagtact tgttgcggcg
     2701 gcggcaaaaa cagcgggcta atcagttgca gcgccgccac cgtccaccat acagtgagta
     2761 aaaccgctaa cgtgccaatg cttaaggtca cctgacgcga gagcggccag cgccatttca
     2821 gccgccgcga atgcagtttt tcattaatga gcacactcat gagaacgcct cccgttgctc
     2881 aaatacgcgg cttaaaacat attcgcgcat ggcgatgaat tgtggatcgg acttgatgct
     2941 gcggctcgac tctcccgcaa caaagcggcg agcaaagttg agcggcagcc gctccagcac
     3001 acggccaggg ccggatgaaa gcagaaccag ttcagtcgcc ataaacaccg cttcttctat
     3061 atcgtgggta atcaacagca cctgcttgcc cgtctcctgc cagagtttca gcagcagagt
     3121 ttgcatctgg tcgcgggtga aggcgtccag cgcaccaaac ggttcgtcga gtaataacag
     3181 ctggggattc gccgccagcg cgcgggcaat ccccacccgc tgacgttgac caccggaaag
     3241 ctgccagata tagcgttttt ctgcgccttc cagccccact tttttcagca tctggtgcgc
     3301 gatttccagt cgctgcattt tctctatacc tgccaattgc aggccgaacg ccacgttgtc
     3361 ctgtacattg cgccacggta gtagcccttc attctgaaaa accaccccgc gctctgctcc
     3421 cggtccctca atacgcttac ccgccagttg aatgctgcca tgctgataag gcacaaaacc
     3481 ggcaatcaga ttcagcaggg tggttttacc gcagccggac ggccccagca ccaccagtag
     3541 ctcgccgctt tccagcgtca ggttgatatc ttccagtgcc ggtttgccgc cataatcggc
     3601 atagagatga gagatttgca gcatctgcgc ctccttttat tgcacgaagc gcgaggtaac
     3661 gtactggctg taatcattcg ctacagccgg gaccttgccc tgctctttca aaaactgcgc
     3721 ggtgtcgatg attgctttgt tcaccggtcc ggtcagttct gccgtttgtt gctgcggcgt
     3781 cagataggta ttccccttca ccagccccgg aacgtcacct tccggcacgc cgcttaaacg
     3841 cgccagtttg ctgatgtttt ccggctgttt cagccacgcg tctgggttag caatgtacgg
     3901 ttgctgagca tcgatggcgc ttttagcgaa cgctttcacg acctcaggat gtttctcggc
     3961 aaaatctttg cgcaccaccc agacgtccag cgttggcgca ccccactgcc cgacctgttc
     4021 agaatcggtc aacaccttgc cgtctttttc cagggcgtta accgccggtg cccagacata
     4081 agcaccatca atatcacccc gctgccatgc ggcgataatc gcgggcggct gcaggttcac
     4141 aatctccact tgccctggtt taatccccca gtgtttcagc gccgccagca ggctgtagtg
     4201 ggtggtggag ataaacggta cggcaatgcg tttgccaatc aaatcttcgg gtttgctgat
     4261 agttttcttt accaccagcg cttcggagtt acccagtttt gacgccagca agaagacttc
     4321 aatcggcacc tgttggctgg ctgcaaccgc taatgggctg gaaccgaggt tgccgatttg
     4381 cacgtcgcca gaagccagcg cccgcacgat gctggctccg ctgtcaaact tacgccagtc
     4441 cacgctcgcc ccgctctctt tagcaaaggt gttgtcggcc tgagccactt tcgccggttc
     4501 ggctgaggtt tgatacgcca cggtgacgtt caccgcctgt gcctgaaaag cgatgaatgc
     4561 cagtgcggca agaagtgtgt tacgcgatga aattgccatg attgtctgct cccctgtctt
     4621 gttatgggag cagtattcag gaataaaaac attcattaaa agaattagtc gttatcgcac
     4681 agatgatttt attcttagca aaaaaacggc aaaggaaaga tt
//
LOCUS       DAEEKQ010000241         4646 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.244, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000241 DAEEKQ010000000
VERSION     DAEEKQ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.244"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            21..2201
                     /locus_tag="KLK57_003969"
     CDS             21..2201
                     /locus_tag="KLK57_003969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000741440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HBE0894203.1"
                     /translation="MAASPKKGTIAWSILSSHNTSGNMDKLKIKFDSLASHDITFVGI
                     VQTAKASGMERFPLPYVLTNCHNSLCAVGGTINGDDHVFGLSAAQRYGGIFVPPHIAV
                     IHQYMREMMAGGGKMILGSDSHTRYGALGTMAVGEGGGELVKQLLNDTWDIDYPGVVA
                     VHLTGKPAPYVGPQDVALAIIGAVFKNGYVKNKVMEFVGPGVSALSTDFRNSVDVMTT
                     ETTCLSSVWQTDEEVHNWLALHGRGQDYCQFNPQPMAYYDGCISVDLSAIKPMIALPF
                     HPSNVYEIDTLNQNLTDILREIEIESERVAHGKAKLSLLDKVENGRLKVQQGIIAGCS
                     GGNYENVIAAANALRGQSCGNDTFSLAVYPSSQPVFMDLAKKGVVADLIGAGAIIRTA
                     FCGPCFGAGDTPINNGLSIRHTTRNFPNREGSKPANGQMSAVALMDARSIAATAANGG
                     YLTSASELDCWDNVPEYAFDVTPYKNRVYQGFVKGATQQPLIYGPNIKDWPELGALTD
                     NIVLKVCSKILDEVTTTDELIPSGETSSYRSNPIGLAEFTLSRRDPGYVGRSKATAEL
                     ENQRLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(2435..3718)
                     /locus_tag="KLK57_003970"
     CDS             complement(2435..3718)
                     /locus_tag="KLK57_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HBE0894204.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNMYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(3853..4545)
                     /locus_tag="KLK57_003971"
     CDS             complement(3853..4545)
                     /locus_tag="KLK57_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0894205.1"
                     /translation="MLNGYIDEGKAASAKLIRSTLSDAFREAIAEGHITTKPVAATRA
                     AKSEVRRSRLTADEYLKIYQTAESSPCWLRLAMELAVVTGQRVGDLCEMKWSDIVDGY
                     LYVEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIASTRREPLSSGTVS
                     RYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLGHKSDTMASQYRD
                     DRGREWDKIEIK"
BASE COUNT         1109 a         1151 c         1199 g         1187 t
ORIGIN      
        1 tcatacacta aatcagtaag ttggcagcat cacccaaaaa aggcactatt gcctggtcta
       61 ttctctcttc tcataatacg tccggaaata tggataaact taaaattaag tttgattcat
      121 tagcctctca cgatattacc tttgttggta ttgtacagac cgctaaagcg tccggaatgg
      181 aacgtttccc gctgccgtat gtgctgacca actgccataa ctcactctgc gccgtcggcg
      241 gcaccattaa cggtgatgac catgtttttg gtttatcggc agctcagcgt tatggcggta
      301 tttttgtgcc tccgcatatt gcggtcatcc atcaatatat gcgtgagatg atggcaggcg
      361 ggggcaaaat gatcctcggg tcagacagcc acacccgtta cggtgcatta gggacaatgg
      421 cagtcggtga gggtggcggt gagttggtaa aacagctgct taatgacacc tgggatatcg
      481 actatccggg cgtggttgcg gtgcatctga ccggaaaacc tgcgccgtat gtggggccac
      541 aggatgtggc gctggctatc attggcgcgg tgttcaaaaa cggttacgtc aaaaacaaag
      601 tcatggagtt cgttggaccg ggcgttagcg cgctctctac cgatttccgt aacagcgttg
      661 acgtgatgac cacggaaacc acctgtttaa gctccgtctg gcaaactgat gaagaagtgc
      721 ataactggct ggcgctgcac ggtcgcggcc aggattactg ccagtttaac cctcaaccga
      781 tggcgtacta cgatggctgc atcagcgttg atttaagcgc catcaaacca atgattgcgc
      841 tgccgttcca cccgagcaac gtgtatgaaa tcgacacact gaaccagaac ctgaccgaca
      901 ttctgcgtga gattgaaatt gagtccgaac gcgtggcgca cggtaaagcc aaactctcgc
      961 tgctggataa agtggaaaat ggtcgcctga aagtgcagca ggggattatc gcgggctgtt
     1021 ctggcggtaa ctacgaaaac gtcatcgcgg cggcgaatgc actgcgcggt caatcctgtg
     1081 gcaatgacac cttctcgctg gcagtttacc cgtcatcaca gccggtgttt atggatctcg
     1141 ccaaaaaagg tgtggtagca gatttgattg gcgcaggcgc aatcatcaga accgcgttct
     1201 gcggcccatg ctttggcgcg ggcgatacgc caatcaacaa cggtttgagt attcgccaca
     1261 ccacgcgtaa cttcccgaac cgcgaaggct ctaagccagc taatgggcag atgtcagcgg
     1321 tggcgttgat ggacgctcgt tctatcgctg cgactgcggc aaacggtggc tatttaacct
     1381 ctgccagcga acttgattgc tgggacaacg tgccggagta cgccttcgat gtaacgccgt
     1441 ataaaaaccg tgtttatcag ggctttgtga aaggggcaac tcagcaaccg ctgatttacg
     1501 ggccgaacat taaagactgg ccggaattgg gtgcgctgac tgacaatatc gtcctgaaag
     1561 tgtgctcgaa gatcctcgac gaagtgacca ccaccgacga actgattcct tccggtgaaa
     1621 cctcttctta tcgttcaaat ccgattggtc tggcggagtt taccctgtct cgccgtgatc
     1681 ccggttatgt tggcagaagt aaagcgactg ctgagctgga aaatcagcgt ctggcgggga
     1741 atgtcagcga gctgacagag gtgtttgcgc gcattaagca gattgctggt caggagcata
     1801 ttgatccgct gcaaactgaa attggcagca tggtctatgc ggtgaaacca ggcgatggtt
     1861 ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg cggtctggcg aatattgccg
     1921 aggagtacgc gactaaacgc taccgttcta acgtcatcaa ctgggggatg ttacctctgc
     1981 agatggcgga agtgccaacc tttgaagtgg gggattacat ttacatccct ggcattaaag
     2041 cggcgctgga taatccgggt acgacgttta aaggttatgt gatccatgaa gatgcgccgg
     2101 taacggaaat tacgctctat atggaaagtc tgactgctga agagcgcgag attatcaagg
     2161 cgggtagttt gattaacttc aataaaaacc gtcagatgta aaaagcgcca tgtgaatgta
     2221 ggtcgcattc ggcacttatt gtcggatgcg atgcttgcgc atcttatccg acctacgaat
     2281 cgcatcgaat ctgtaggcca gataaggcat ttacgcagca tccggcactt attgtcggat
     2341 gcgatgcttg cgcatcttat ccgacctaca aatcgcatcg aaccgtaggc cggataaggc
     2401 gtttacgccg catccggcaa atagttaatt gctcttactt cttcgcctct gcaaccactt
     2461 tactacccac gccgcggtta ttgtattccc acatgcggtt gtagttagtg tcattcagat
     2521 tgcgctgtat ttcgtcgtta tcatctacgc tgccggtatt acccgcaaac ggacgattag
     2581 agatcaccgc atcggcccac ggtttagccg tgttaaaacc ttcgttgatg gcgctatcac
     2641 ggatcaccac ctgaccgttg gtattggcat caacatccag cgagcggccc agttgcgcca
     2701 caccatcacc gaaagcattg aaacggctgt ttacggcgag gaaaccgtag tacatgttgg
     2761 acagcgtagc cggtgcaaac acatacgctt cttgctgagt acgtgagttc accacgcgga
     2821 attcggtgtt atcgaacacc actgcgccgc gaccagaaac gatatccaca tccccttcaa
     2881 tgtagctgtt ggtcaccagc gtacgcggct gacgattcgt ttccagacgg ttctgcacac
     2941 cgctgttggt gacaaagaag gtgttctgac gaccgagaat gttaacgttg ttaatctgta
     3001 cctggtcacc atcagtacgc agtgccaccg ccggatggtt acctgcatct acgctatcgc
     3061 ccagcgtgtt ttcgatggtc agattttgca gttgcaggcc attgttttgt gaccagaaga
     3121 ccgcagagca gagaacaccg atactgtcgc tgcgtttgct ctggcagcta tcgtacatat
     3181 accacgctgg tttacctggc atatatttgc cgcgcgggtt gacgtcgtga cgccagtcgg
     3241 cagggctcat gccaccatca agggaaagcc caatcttcac atcaatcggt ttttcacctg
     3301 taccgtacag agtaattcca cccggagcgg cagggacata taccgttccc tgatactcac
     3361 caggcatcac ggcaatatac tggcgcttgt tggtacgctt gataattgcc gcatctaccg
     3421 ccgcctgaat cgtggtatgc gttacacctt gagtgcccgc cgggccgaca acaaagtcag
     3481 gttgcgcagg cagggtaatc ggggaaggat tccacgctgc agcacctggt gtcagggatg
     3541 caaaatagtg ttgagcatcg aaattctgcg cttcttttgc cgacagaatc gggcgagaag
     3601 aggtaccagg cgcggtttga tcagaaggac gttgatcggg cggggttgag ctacaggcgg
     3661 tcagcgtcac gccaaaagcc aatgccagcg ccagacggga aactgaaaat gtgttcacag
     3721 gttgctccgg gctatgaaat agaaaaatga atccgttgaa gcctgctttt ttatactaag
     3781 ttggcattat aaaaaagcat cgcttatcaa tttgttgcaa cgaacaggtc attatcagtc
     3841 aaaataaaat cattatttga tttcaatttt gtcccactcc ctgcctctgt catcacgata
     3901 ctgtgatgcc atggtgtccg acttgtgccc gagaagatgt tgagcaaact tatcgcttat
     3961 ctgcttctca tagagtcttg cagacaaact gcgcaactcg tgaaaggtag gtggatcccc
     4021 ttcgaaggaa agacctgatg cttttcgtgc gcgcataaaa taccttgata ctgtgccgga
     4081 tgaaagcggt tcacgacgag tagatgcaat tatggtttct ccgccaagaa tctctttgca
     4141 tttatcaagt gtttccttca ttgatatccc gagagcatca acatgcaatg ttgtagggat
     4201 ggcaattttt acgcctgttt tgctttgctc gacataaaga tatccatcta cgatatcaga
     4261 ccacttcatt tcgcataaat caccaactcg ttgcccggta acaacagcca gttccattgc
     4321 aagtctgagc caacatggtg atgattctgc tgtttgataa attttcaggt attcgtcagc
     4381 cgtaagtctt gatctcctta cctctgattt tgctgcgcga gtggcagcga ccggttttgt
     4441 tgttatatgg ccttcagcta ttgcctctcg gaatgcatcg ctcagtgttg atctgattaa
     4501 cttggctgat gccgccttgc cctcgtctat gtatccattg agcattgccg caatttcttt
     4561 tgtggtgatg tcttcaagtg gagcatcagg cagccccctc cttattgctt taattttgct
     4621 catgtaattt atgagtgtct tctgct
//
LOCUS       DAEEKQ010000242         4544 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.245, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000242 DAEEKQ010000000
VERSION     DAEEKQ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4544
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.245"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            133..651
                     /locus_tag="KLK57_003972"
     CDS             133..651
                     /locus_tag="KLK57_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBE0894206.1"
                     /translation="MPTPCYISITGQTQGNITAGAFTADSVGNIYVQGHEDEMLVQEF
                     LHNVTVPTTPQSGQPAGQRAHKPFIFTVALNKAVPLMYNALASGEMLPTTELHWWRTS
                     VEGKQEHYFTTRLTDSTIVDMKLHMPHCQDPAKREFTQLLEVSLAYRKIEWEHVKSGT
                     SGADDWRAPLEA"
     gene            861..3002
                     /locus_tag="KLK57_003973"
     CDS             861..3002
                     /locus_tag="KLK57_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HBE0894207.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLELSLVSQQ
                     FLSLEFPQVLDKMAYLTIWQGDDVQRRVKGVVTWFELGENDKNQMLYSMKVCPPLWRT
                     GLRQNFRIFQNEDIESILATILKENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAQKSTDQSLVLCDTVRYLPESFEIPWNPNTRTEVSTFCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWAGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPSNQDSSCWIRVAQAWAGTGFGNLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYKGSGFNELKFDDATGKEQVYIHVQKNMNTEVLNN
                     RTTDVINNHAEKIGNNQAITVTNNQIQNIGVNQIQTVGVNLVETVGSNQIIKVGSNQV
                     EKVGIIRALTVGVAYQTTVGGIMNTSVALLQSSQVGLHKSLMVGMGYSVNVGNNVTFS
                     VGKTMKENTGQTAVYSAGEHLELCCGKARLVLTKDGSIFLNGTHIHLEGESDVNGDAP
                     VINWNCGATQPVPDAPVPKDLPPGMPDMRQF"
     gene            3078..>4544
                     /locus_tag="KLK57_003974"
     CDS             3078..>4544
                     /locus_tag="KLK57_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBE0894208.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGI
                     TYANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFD
                     IRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALW
                     QVLPEDVRLSPHVYLATNSLQGPWWILSWPEPPAYRVLTVVVDGFGRSLTFHRAAEGD
                     VAGAVTGVTDGAGRRFHMALSTQAQRAEASRKQRASSLSSPASPRSVSSSQVFPDTLP
                     AGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVYDRSGTQVRGF
                     TYDAEHAGRMVAHHYAGRPESRYRYDDTGRVTEQVNPEGLDYRFEYGESRVIITDSLN
                     RREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRRTEYRLHMASGK
                     LTSVVLPDGRTVRYGYNNQLQLTSVTYPDGLRSSRKYDR"
BASE COUNT         1035 a         1183 c         1422 g          904 t
ORIGIN      
        1 ttctagctcg gtttgtatgt aaaaaatact gaaaaatgat gcggttccgt gaagtgagct
       61 gcatctgcct gtctgggatt aacggcaggt cattgtaagt agtaattaac cacgttaaca
      121 aggagtttac aaatgccaac accgtgttat atctccatca ctggtcagac ccagggaaat
      181 attactgcag gtgcatttac cgctgattct gtcggcaaca tctatgtgca ggggcacgaa
      241 gacgagatgc tggtgcagga attcctgcat aacgttactg tcccgaccac tcctcagtcc
      301 ggtcagcctg cgggtcagcg tgctcacaag cctttcattt ttaccgtggc gctgaacaaa
      361 gcggttccgc tgatgtacaa cgcactggcc tccggcgaaa tgctgccaac cactgaactt
      421 cactggtggc gtacctctgt ggagggcaaa caggagcatt atttcactac ccgcctgact
      481 gattcgacga ttgtcgatat gaaactgcat atgccgcatt gccaggaccc ggcgaaacgc
      541 gagttcaccc agttgctcga ggtttcactg gcctaccgca aaattgaatg ggaacacgtc
      601 aaatctggca cctccggtgc cgacgactgg cgcgcaccac tggaagcata agttaagcca
      661 acagcatccg gctatcaggc cggatgctgt ttttgtatta cgccgtagaa agaaaggttg
      721 tcagtaaggg gttacgttca cgggagatgg cggatgccgg aaacttcggc ggtagcgtgc
      781 ctgaacgcaa gcgtaacact gtactgtcac agccgcttta acagcgaact gtctgttttt
      841 ccttaacccc ggaggttgct atgtcaaccg gattacgttt cacgctggaa gtggacggcc
      901 tgccaccgga tgcttttgcg gtggtctcct ttcatctgaa ccagtcactc tcttcgctct
      961 tttccctcga gctctctctg gtcagccagc agtttctctc ccttgaattt ccgcaggtac
     1021 tggacaaaat ggcctacctg acgatatggc agggcgatga cgtacagcgc cgggtgaaag
     1081 gtgtggtgac ctggtttgaa ctgggggaga acgacaaaaa ccagatgctg tacagcatga
     1141 aggtatgccc accgctgtgg cgcacagggc tgcgccagaa cttccgtatc ttccagaatg
     1201 aggacatcga aagcatcctc gcgacgatcc tgaaggaaaa cggtgtgacc gagtggagtc
     1261 cgctgttcag cgagccgcat ccttcccgtg agttttgtgt ccagtacggc gaaactgatt
     1321 acgatttcct gtgccggatg gcggcggagg aaggcatctt cttttatgag gagcacgcgc
     1381 aaaaaagtac cgaccagagc ctggtcctgt gcgacaccgt gcgttatctg ccggagtcct
     1441 ttgagatccc ctggaacccg aacacccgta ccgaggtaag taccttctgc atcagccagt
     1501 tccgctacag cgcacaaatc cgtccttctt ccgtggtgac caaagactac acctttaaac
     1561 gacccggctg ggcagggcgt tttgatcagg aaggccagta ccaggattac cagcgcacac
     1621 agtatgaagt gtatgactac cccggacgtt tcaagggtgc tcacgggcag aactttgccc
     1681 gctggcagat ggatggctgg cgcaacaacg cagaagtggc gcgcggaaca agccgttctc
     1741 cggagatatg gccgggacgg cgaattgtgc tgacggggca tccgcaggcg aacctgaacc
     1801 gggaatggca ggtggtggca agtgaactgc acggcgagca gccgcaggcg gtgccgggac
     1861 gcagtggttc aggaaccacg ctgaataacc actttgcggt aataccggca gaccggacat
     1921 ggcggccaca gccgttgctg aaaccgctgg tggatggccc gcagagcgcc gtcgtgacgg
     1981 gaccggcagg cgaggaaatc ttctgtgatg aacatggccg cgtgcgggtg aaatttaact
     2041 gggaccgcta taacccgtca aaccaggaca gttcatgctg gatccgtgtg gcacaggcgt
     2101 gggcaggcac cggattcggt aaccttgcga taccgcgcgt gggtcaggag gtcattgtgg
     2161 acttcctcaa cggcgatccg gaccagccga ttattatggg gcgtacctac caccaggaaa
     2221 accgcacacc cggtagcctg ccggggacga agacgcagat gaccattcgt tcgaaaacct
     2281 ataagggcag cgggtttaat gaactgaagt ttgacgatgc gacagggaaa gaacaggtct
     2341 acatccacgt gcagaagaac atgaacaccg aggtgctgaa taaccgcacc acggatgtga
     2401 taaacaacca tgccgaaaaa ataggtaaca accaggcgat caccgttacc aataaccaga
     2461 tccagaacat tggcgttaat cagatacaga cggttggtgt caacctggtg gaaacggtgg
     2521 gcagtaacca gattatcaaa gtgggatcaa accaggttga aaaggtgggg atcattcgtg
     2581 cgctgacggt gggtgtggct taccagacga cggtaggcgg cattatgaat acctcggtgg
     2641 cgttgttgca gtcctcacag gtagggctgc ataaatcact gatggtggga atgggctaca
     2701 gcgtcaatgt agggaataac gtcaccttct cggtgggcaa gacgatgaag gaaaacaccg
     2761 gacaaacagc agtttattct gccggtgagc atcttgaact ctgctgtggt aaggcaaggc
     2821 tggtgctgac gaaggacgga agcatatttc tcaacggtac gcacattcat ctggaagggg
     2881 agtcggatgt gaacggtgat gcgccagtga ttaactggaa ctgtggtgcc acacaacctg
     2941 taccggatgc gcctgtgccg aaagatttac cccccggaat gccggatatg cggcaatttt
     3001 gagtcaggtg caaaaaatta aaacggaact ggtgcagtga ccagatgaac catattaaaa
     3061 gcagaaggaa ttgattcatg ggcggcggaa aaccggcggc gcggcagggc gacatgaccc
     3121 gcaagggact ggatattgtg cagggttcag cgggtgtgct gataggtgcg ccgacgggag
     3181 tggcctgttc ggtgtgtccg ggagggatta cctatgctaa cccggtgaac ccggtgctgg
     3241 gtgcgaaggt gcttccgggc gagacggacc ttgcgctgcc ctgcccgctg ccgtttattc
     3301 ttttccgcgc ctacagcagc taccggacca gaacacccgc gccggtgggg gtgtttggtc
     3361 ccggctggaa agcgcccttc gatatccgct tacagatacg cgatgaaggc ctgatactca
     3421 acgacagcgg cggcaggagc attcactttg agccgctgtt tcccggcgag ataagctaca
     3481 gccgcagcga gtcactctgg ctggcgcggg gcggagtggc ggcgcagcac agttcgcagc
     3541 cgctaagcgc gctctggcag gtgctgccgg aagatgttcg cctgagtccg catgtgtacc
     3601 tggcgacaaa cagccttcag gggccgtggt ggatactcag ctggccggag ccgcccgcat
     3661 accgggtact gacggtcgtg gtggatggct tcgggcgctc actgactttc caccgggcgg
     3721 cggagggtga cgtggcgggc gcggtgacgg gggtgacgga cggcgcgggc cgccgttttc
     3781 acatggcgct gagcacgcag gcgcagcggg cggaagcgtc ccgtaaacag cgcgcctctt
     3841 ccttatcttc cccggccagt ccccgttctg tttcctcttc tcaggttttc cccgacacgc
     3901 tgcccgccgg tacagagtac ggtgccgata acggtatccg gctggaggcg gtatggctga
     3961 cacacgaccc ggcatacccg gatgaacagc cgactgcgcc gctggcgcgc tacacgtaca
     4021 cggccggtgg tgaactgcgg gcggtgtatg accgcagcgg gacgcaggtt cgcgggttta
     4081 cttatgatgc ggagcacgcc gggcggatgg tggcgcacca ttatgcgggg aggccggaga
     4141 gccgctaccg gtatgatgat accggtcggg tgacggagca ggtcaacccg gaggggctgg
     4201 actaccgctt tgagtacgga gagagccggg taatcatcac ggacagcctg aaccggcggg
     4261 aggtgctgta cacggaaggc gagggtggcc tgaagcgggt ggtgaagaag gagcatgccg
     4321 acgggagcat cacccgcagc gagtatgatg aggcggggag gctgaaggcg cagacggatg
     4381 cggcggggcg gcggacggag taccggctgc atatggcgtc tggaaagctg acttcagtgg
     4441 tactgccgga cggcaggacg gtacggtatg gttacaacaa ccagttgcaa ctgacgtcag
     4501 tgacatatcc ggatggttta cgcagcagcc ggaaatatga ccgt
//
LOCUS       DAEEKQ010000243         4528 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.246, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000243 DAEEKQ010000000
VERSION     DAEEKQ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4528)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4528)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4528
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.246"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(139..3654)
                     /locus_tag="KLK57_003975"
     CDS             complement(139..3654)
                     /locus_tag="KLK57_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE0894209.1"
                     /translation="MDKNALGFASYWRNSLADAESGKGSFERKDAKNFTHWHGIAAGR
                     LDEAIVSKFFKGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLIVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4106..4408)
                     /locus_tag="KLK57_003976"
                     /pseudo
     CDS             complement(4106..4408)
                     /locus_tag="KLK57_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT         1167 a         1159 c          933 g         1269 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagatgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg ataccttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca ttaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcgtcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaggagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aatcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcacct gcagaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc ttaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt tggcgtcttt ccgttcaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcatttttat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aaccaccata tggacaacta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcggtat caaatgtact
     3781 ggttgtacaa ggttcagaac acaagcacat tcaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa tgactgcctg tgttggacaa ggataccaga
     3901 acagccgaaa acaccggggg ttattacctg ctctcatgga aaccagatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacat gacgaaggga ctttaatgga agccggagta
     4021 acttgccaga acatcaatct tcgtcatcat ttacaaagaa aaataatcag ttaaagcagt
     4081 ccgggcaatc agcccggact gcttactatt ttaatggatt gttaatcttt gcatattcaa
     4141 acgggctgat acccttatct ctgttcgcat ccagaaaatc tgcccaccac tgtagcatca
     4201 gtcgacgttc ttccagatgt tctgctttat ggatatacgc agcacgtaca ccattacgct
     4261 cctgatggct catctgacgt tccacagcat cgcgggacca caaacctgat tcaatcaatg
     4321 cactgcacgc catcgcccgg aatccatggc cacagacatc ctgggttgtg tcatacccca
     4381 tcaccctcag ggctttattt acagtattta tatgaacgca tcccgtttgc aaggcatcca
     4441 ttgttttgac atgacgggta ggttgcagct ttatatccgg ctttattgca gccgtaacgc
     4501 tgcgagcccc tatgtaattc gccgactc
//
LOCUS       DAEEKQ010000244         4523 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.247, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000244 DAEEKQ010000000
VERSION     DAEEKQ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4523)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4523)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4523
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.247"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..2658
                     /locus_tag="KLK57_003977"
     CDS             1..2658
                     /locus_tag="KLK57_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001674509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase/endonuclease Cas3"
                     /protein_id="HBE0894210.1"
                     /translation="MTFFDFWGKTRRGEKDGGDDYHLLCWHSLDVAAMGYLMVKRNCF
                     GLADYFRQLGISDKEQAAQFFAWLLCWHDIGKFARSFQQLYLPPELKIQEGARKNYEK
                     ISHSTLGYWLWNHYLSECQELLPSSSLSPRKLRRVIEMWMPVTTGHHGWPPDRMDELD
                     NFLPEDKAAARDFLLEIKPLFPLIEIPAFWDDDEGIELIKHLSWYISATVVLADWTGS
                     STRFFPRVAHPMDIKGYWQKTLVQAQNALTVFPLKAKVAPFNGINTLFPFIENPTPLQ
                     QKVLDLDISQQGPQLFILEDVTGAGKTEAALILAHRLIAAGKAQGLFFGLPTMATANA
                     MYDRLVKTWLAFYSPESRPSLVLAHSARTLMDRFNESLWSGDLVGSEEPDEQTFSQGC
                     AAWFADSNKKALLAEIGVGTLDQAMMAVMPFKHNNLRLLGLSNKILLADEIHACDAYM
                     SCILEGLIERQARGGNSVILLSATLSQQQRDKLVAAFARGTEGLQEAPFLEKDDYPWL
                     THVTKSDVNSHRVATRKDVERSVSVGWLHSEQECIARIESAVSQGKCIAWIRNSVDDA
                     IKVHRQLLARGVIPASSLSLFHSRFAFSDRQRIETETLARFGKEDSSQRAGKVLICTQ
                     VLEQSVDCDLDEMISDLAPVDLLIQRAGRLQRHIRDINGQLKRDGKDERSPPELLILA
                     PVWDDSPGDEWFGSAMRNSAFVYPDHGRIWLTQRVLREQGAIQMPHAARLLIESVYGE
                     DVVMPEGFARSEQEQVGKYYCDRAMAKKFVLNFRPGYAANINDYLPEKLSTRLAEESV
                     SLWLATCIDGVVKPYATGAHAWEMSVVRVRRSWWKKHRDEFSLLEGEAFRLWCIEQRQ
                     DPEMANVILVNDDESCGYSATEGLIGKVG"
     gene            2756..4317
                     /gene="casA"
                     /locus_tag="KLK57_003978"
                     /pseudo
     CDS             2756..4317
                     /gene="casA"
                     /locus_tag="KLK57_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse1/CasA"
     gene            4314..>4523
                     /locus_tag="KLK57_003979"
     CDS             4314..>4523
                     /locus_tag="KLK57_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HBE0894211.1"
                     /translation="MSIVKEEHKATLRKWHEELQEKRGERASLRRSTTVNDVCLTDGF
                     RLFLKNRQIKWQDEPEWRITALALIA"
BASE COUNT         1121 a         1046 c         1243 g         1113 t
ORIGIN      
        1 gtgacttttt ttgatttctg gggaaaaacc cgacgcggcg agaaagacgg gggcgacgac
       61 tatcaccttc tttgctggca ttcgctggat gtagccgcga tgggctattt aatggttaaa
      121 agaaattgct tcgggctggc tgattatttt cgtcaattag ggatttctga caaggaacag
      181 gcggctcaat ttttcgcctg gttgctgtgc tggcacgata ttggaaaatt tgcccgctct
      241 tttcaacaac tttacctgcc ccctgaactc aagattcagg aaggtgccag aaaaaattac
      301 gaaaaaatct ctcattcaac gctgggctac tggttgtgga atcattattt aagtgaatgt
      361 caggagttac ttccttcatc ttcgctctct ccgcgcaaac ttaggcgagt gatagaaatg
      421 tggatgcccg tgacgacagg gcatcatggt tggccccctg atcgtatgga tgaattggat
      481 aattttctgc ctgaagacaa agctgcagca cgagattttc tccttgaaat caaaccactg
      541 tttccgctca tagagatccc cgcattctgg gatgatgatg agggcattga acttataaaa
      601 cacctttcct ggtatatctc tgcaaccgtc gtgctcgcag actggacggg ttcgtcaacg
      661 cgattttttc cacgcgtcgc acacccaatg gatattaaag gttactggca gaaaacttta
      721 gttcaggctc aaaacgcctt aaccgtcttt cctctaaaag caaaagtcgc gcctttcaac
      781 ggaattaata cgctgtttcc ttttattgag aatccgacac cgttacagca aaaggtgctg
      841 gatctggata tcagccagca aggaccgcag ttatttattc tggaagatgt gactggcgca
      901 ggtaaaacag aagcggcgct tatcctggcg cacaggttga tagctgcggg gaaggctcag
      961 gggctgtttt ttggtctgcc aacaatggca acggccaatg ccatgtacga tcggctggtc
     1021 aaaacctggc ttgctttcta ttcgccagaa tcccgcccca gcctggtgct ggcacatagt
     1081 gcccgcacat taatggaccg ctttaatgaa tcactctggt ctggtgattt agtcgggtca
     1141 gaagaaccgg atgaacaaac gttcagtcag ggatgtgcgg cctggtttgc cgacagtaac
     1201 aagaaggcgt tgctggctga aattggcgtc ggcacgctgg atcaggcgat gatggcggtg
     1261 atgccgttca aacataataa tctgcggctt ctggggctga gtaacaaaat cctgctggct
     1321 gatgagatcc acgcctgtga cgcttacatg tcgtgcattc ttgaagggtt gatcgagcgg
     1381 caggcgcgtg gcggaaacag cgttattttg ctttctgcca cgttatctca acagcagcgc
     1441 gacaaactcg tcgctgcctt tgcgcggggt acagagggcc tgcaagaagc tccgttcctg
     1501 gaaaaggatg attacccctg gctgacgcat gtcacgaaat ccgatgtgaa ctcacaccgg
     1561 gtagcgacgc gcaaagacgt tgagcgtagc gtcagcgtgg gttggcttca tagtgaacaa
     1621 gagtgtattg cgcgtatcga atcggcggta agtcagggga aatgcatcgc ctggatccgg
     1681 aattctgtcg atgacgctat taaggttcat cgtcagctgc ttgcccgcgg cgtcattcct
     1741 gcttccagcc tttcactttt tcatagccgc tttgctttta gcgatcgcca gcgaattgaa
     1801 acggagacac tggcacgctt tggtaaagaa gatagctcac agcgtgccgg aaaagtcctt
     1861 atttgtactc aggtattaga gcagagtgtc gattgtgacc tggacgaaat gatctccgac
     1921 ctggcccctg ttgatttgct gattcagcga gcaggacgat tacagcggca tatccgcgat
     1981 attaatggtc agttaaagcg tgacggaaag gacgagcgtt ccccccctga attgctgatt
     2041 ctggctcccg tctgggacga ctctcctggc gacgaatggt ttggcagtgc catgcgtaac
     2101 agtgcatttg tctatcccga tcatgggcga atctggctga cgcagcgtgt gctgcgtgag
     2161 caaggtgcta ttcagatgcc acacgcagcc cggcttctta ttgaatccgt ctacggtgag
     2221 gacgtggtaa tgccggaagg atttgcccgc agcgagcagg agcaagtggg caaatattac
     2281 tgtgatcgcg caatggctaa aaagtttgtg ctgaacttca ggcccggcta tgccgccaat
     2341 atcaacgatt acctcccaga aaaactgtcg acacgtctgg ctgaggagtc tgtttccctg
     2401 tggctggcta cctgtattga cggtgtggtg aagccttatg ccaccggtgc tcacgcatgg
     2461 gaaatgagcg ttgtcagagt gcgccgaagc tggtggaaaa aacatcggga tgagttttct
     2521 ttactggagg gagaggcgtt caggctgtgg tgcattgaac agcgacaaga tccggagatg
     2581 gcaaacgtta ttttagtcaa cgatgacgaa agttgtgggt attcggctac ggaaggattg
     2641 attggcaagg ttggttgaga tgtctgcgac aggactctct gaatagttga tacaccgaaa
     2701 aggatggaaa gatggacaac cgacctggga tggtcttttt tacgagagga tgcgtatgaa
     2761 ctcgttttca cttctgacaa ccccgtggtt gcccgttcgt tttaaagacg gaacgacagg
     2821 caaactggcg ccagtcgatc tggcggatga aaatgttgtc gatatttctg cgccgcgggc
     2881 agatctccag ggtgcggtat ggcagttttt gctggggcta ctacaaacca gcttcgcgcc
     2941 aaaagatcat cgtcgttggg atgatatctg ggaagacgga ctggaagctg aaaagctacg
     3001 ggaagcattg ctgtcattag aacatgcttt ccagtttggg ccagattccc cttcatttat
     3061 acaggatttc gaggcgctca ccggagataa agttccggtc gcttcgctac tgcctgagat
     3121 tcccggcgct caaacaacga agtttaataa agaccacttt atcaagcgtg gtgtaacgga
     3181 atacctgtgc ccacattgct ctgcgttagc tctgttctcc ctacagttga atgcgccgtc
     3241 aggtggcaaa ggctaccgca ccggtttacg cggcggtggg ccgatgacca ctctgattga
     3301 attacaggaa tatcagggta atcaacaaac tcccttgtgg cgcaaactgt ggatcaatgt
     3361 catgccgcag gatgaagccg atttaccgct acccaaaaaa tttgacgatc tggttttccc
     3421 ctggcttggc ccgacgcgca tcagcgaact ggccggtgcg gtggtaaccg atgagcaggt
     3481 caataaactc caggcgtact ggggaatgcc tcgccgtatt cgtattgatt ttaataccac
     3541 cacagtcggc aactgcgata tttgcggtga gcagagcgac gcgcttctga gcctgatgac
     3601 gaccaaaaat tacggcgcta actatgccat gtggcagcat cccttaacgc cttaccgtgt
     3661 actacttaaa gagggcggcg agttttactc ggtcaaacca caaccgggcg gtttaatctg
     3721 gcgcgactgg ttaggcctta tcgaaacggg taagtcagaa aacaatacgg aacttcccgc
     3781 gctggtggtg aaactcttta atgccagcag tctcaagcag gcaaaagtgg gcctgtgggg
     3841 atttggctat gatttcgaca acatgaaagc gcgctgttgg tacgaacatc atttcccgct
     3901 gctgctcaat aaaaagaagg ccagataccg aaactgcggc tggctgcgca aacggcttca
     3961 cggattctaa gtctgttacg gagtgcattg aaagaagcat ggttctccga tccaaaaggt
     4021 gcaaggggtg attttagttt tgtggatatc gacttctgga acaaaactca gcatcgcttc
     4081 ctgaggttag tgcgccaaat tgaagaaggt caggatgcgg atgaattact cagcaaatgg
     4141 aataaggaaa tttggttatt cgcgcgtcag gattttgacg agcgtgtatt caccaatcct
     4201 tatgagcccg ttgatttgga acgcatcatg accgcgcgca agaaatattt tacaacatcg
     4261 gcggagaagc aaagtgctaa agccgccagg gagaaaaagc aggaggctgc tgaatgagta
     4321 ttgtaaaaga ggagcacaaa gccacgctgc gcaaatggca cgaggaattg caggagaaac
     4381 gcggcgaacg tgccagtctg cggcgtagta cgacggttaa tgatgtttgt ctgacggatg
     4441 gatttcgcct gtttctgaag aacagacaaa taaagtggca ggatgaacct gagtggcgta
     4501 tcaccgcact ggcgctgata gcc
//
LOCUS       DAEEKQ010000245         4487 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.248, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000245 DAEEKQ010000000
VERSION     DAEEKQ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4487
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.248"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(19..378)
                     /gene="traA"
                     /locus_tag="KLK57_003980"
     CDS             complement(19..378)
                     /gene="traA"
                     /locus_tag="KLK57_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
                     /protein_id="HBE0894212.1"
                     /translation="MNTVLSVQGASAPVEKKSFFSKFTRLNMLRLVRAVIPVAVLMML
                     FPELAMAAGKGDLMAKGNDTVKATFGKDSSIVKWVVLAEVLVGAVMYMMTKNVKFLVG
                     FAIISVFIAVGMSVVGL"
     gene            complement(411..638)
                     /gene="traY"
                     /locus_tag="KLK57_003981"
     CDS             complement(411..638)
                     /gene="traY"
                     /locus_tag="KLK57_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer relaxosome protein TraY"
                     /protein_id="HBE0894213.1"
                     /translation="MSRNIIRPAPGNKVLLVLDDATNHKLLGARERSGRTKTNEVLVR
                     LRDHLNRFPDFYNLDAIKEGAEETDSIIKDL"
     gene            complement(775..1446)
                     /gene="traJ"
                     /locus_tag="KLK57_003982"
     CDS             complement(775..1446)
                     /gene="traJ"
                     /locus_tag="KLK57_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer transcriptional regulator
                     TraJ"
                     /protein_id="HBE0894214.1"
                     /translation="MYPTDPRQLNTERQIYLDKQFFVDVFSIPACVRNTNGDFIGYNE
                     KFSKEFIGSLDIKEWFYSLPVQVATSFLREELDAMSLPSSMNKIQSVAIGDKLWLVQF
                     IPLIYGEVVNVLWLFFCKNSNVIVDYCRGLRTNITNDRMLEFKNKSTEIQWKVFILYS
                     FGFCHESIASLLSITNGSSRNAISEVYKFFGIHSKHDLLMIFHTSRMHSLFFDELFFI
                     LKCAE"
     gene            complement(1640..2023)
                     /locus_tag="KLK57_003983"
     CDS             complement(1640..2023)
                     /locus_tag="KLK57_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxosome protein TraM"
                     /protein_id="HBE0894215.1"
                     /translation="MARVILYISNDVYDKVNAIVEQRRQEGARDKDISVSGTASMLLE
                     LGLRVYEAQMERKESAFNQTEFNKLLLECVVKTQSSVAKILGIESLSPHVSGNPKFEY
                     ANMVEDIREKVSSEMERFFPKNDEE"
     gene            2346..2948
                     /locus_tag="KLK57_003984"
     CDS             2346..2948
                     /locus_tag="KLK57_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HBE0894216.1"
                     /translation="MVLLVVRTTNLLSLFVEWVKLFTDKVTRGGKMKKWMLAICLMFI
                     NGICEAADCFDLAGRDYKIDPDLLRAISWKESRYRVNAIGINPVTGYGSGLMQVDSQH
                     FNELARYGIKPEHLTTDPCMNIYTGAYYLAIAFKKWGVSWEAVGAYNAGFRKTERQNQ
                     RRLAYASEVYRIYTWIKSSKGIRVPTTKKSLSQINSVQKN"
     gene            complement(3245..4066)
                     /locus_tag="KLK57_003985"
     CDS             complement(3245..4066)
                     /locus_tag="KLK57_003985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HBE0894217.1"
                     /translation="MRLASRFGRYNSIRRERPLTDDELMQFVPSVFSGDKHESRSERY
                     TYIPTINIINKLRDEGFQPFFACQSRVRDLGRREYSKHMLRLRREGHINGKEVPEIIL
                     LNSHDGSSSYQMIPGIFRFVCTNGLVCGNNFGEIRVPHKGDIVGQVIEGAYEVLGVFD
                     KVTENMETMKEIHLNSDEQHLFGRAALMARYEDENKTPVTPEQIITPRRWEDKQNDLW
                     TTWQRVQENMIKGGLSGRSASGKNTRTRAITGIDGDIRINKALWMIAEQFREWKS"
     gene            complement(4177..4473)
                     /locus_tag="KLK57_003986"
     CDS             complement(4177..4473)
                     /locus_tag="KLK57_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894218.1"
                     /translation="MSAFSFNTTYQPTGDQQKDAIAQIGIMQNRAVQANLAYQSCRDS
                     GALFKVLHQVNNELHDLLDSLENHTPLVRKNADELIALLLLFTRQVGQSRTDLI"
BASE COUNT         1401 a          990 c          822 g         1274 t
ORIGIN      
        1 acatcgtttt atttcctgtc agaggccaac gacgctcata cccacagcaa taaatacaga
       61 gatgatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca tgaccgcacc
      121 gaccagcacc tcagccagaa caacccattt aacgatactg gagtccttac cgaaggtcgc
      181 cttaaccgta tcgttacctt tagccatcag gtcgccttta ccagcagcca tagctaactc
      241 agggaacaac atcatcagaa cagcaaccgg gatcactgca cgaaccaggc gaagcatatt
      301 cagacgagtg aatttagaaa aaaacgattt cttttcaacg ggcgcagaag caccctgaac
      361 acttaaaaca gtattcataa gttacctcta ttgtggatag attaataaac ctaaagatct
      421 ttaattatcg aatcagtttc ctctgctccc tcctttattg catccagatt ataaaagtca
      481 ggaaaacggt tcagatgatc acgtaacctg actagcacct cattagtttt tgttctccca
      541 ctacgttccc gggcacctaa gagcttgtga ttagtagcat cgtccaatac cagtaaaact
      601 ttattgcccg gagcaggcct tattatattt ctgctcaagt ctcctcctca gccacttaga
      661 taattaaata taattatacg aatccataaa aatagaaacg aaaatagagt aaattacatt
      721 aacaaaacaa ggtgacataa tgtcaccttg tttttaacaa aaacgaaaca tcaactattc
      781 agcacatttt aaaataaaaa acaattcatc aaagaataaa ctatgcatcc tgcttgtatg
      841 aaaaatcatt aacaaatcat gttttgaatg tataccgaaa aacttataca cttctgaaat
      901 tgcattccgg gaagaaccat tagtaataga caaaagagat gctatcgatt catgacaaaa
      961 accaaatgaa taaagaataa aaactttcca ttggatttcc gttgatttgt ttttaaattc
     1021 aagcattctg tcatttgtta tatttgtacg taatcctcta caataatcca ctatcacatt
     1081 agaatttttg cagaaaaaaa gccataaaac attaaccacc tccccatata ttaatggtat
     1141 aaattgtacc agccagagtt tatcacctat agccacgctc tgtattttat tcatggacga
     1201 aggcaaagac atagcatcca gttcttctct taagaacgat gtagctactt gtacaggaag
     1261 ggagtaaaac cactctttga tatcaagact acctataaat tctttagaga atttttcatt
     1321 gtagccgata aaatctccat tagtatttcg aacacaagca gggattgaaa aaacatcgac
     1381 aaaaaactgt ttatccaaat atatttgacg ttcagtatta agttgtctag gatccgtcgg
     1441 atacatagga acctcctctc aaaggattct atggacagtc gatgcgggga ggtcgctctc
     1501 cctgcatcgg cgatttttaa tatctgtttt aacgtggcat taaccacata attaatataa
     1561 cctttatgtg gttaattgtc atcaaattga accagatcaa aatcctgtta tttgaagtaa
     1621 atcaactttt aattcttttt tattcctcat cattttttgg aaaaaatcgt tccatctcag
     1681 atgacacctt ctcacgaata tcttcaacca tattggcata ttcaaacttt ggattcccgg
     1741 agacatgggg acttagagac tcaataccca aaattttcgc tactgatgac tgtgttttta
     1801 caacacattc aagaagaagt ttattaaact ctgtctgatt aaacgcagac tctttacgct
     1861 ccatctgagc ctcatataca cgaagcccca gttcaagaag cattgaagct gttcccgaaa
     1921 cacttatatc tttatctctt gccccctcct gacgacgctg ttcaactatc gcatttactt
     1981 tatcatagac atcattactg atatacaaaa ttactctggc cataagataa aacctttcat
     2041 tattaagcaa tgaacttttc actataaata tgcatatagt gtttacaagt aagaaagaca
     2101 ctcctagcag cgcctctagg atcatcctat aaaaaaatgc gatccggcgc taggggcgtc
     2161 cctaatatat atcaatgttt ttcatgaaaa ttgtcagtac tgatcctaat aagagtcgct
     2221 atagggtcgt aacaggatcg ccaacgactc tctatttaat aattcagaat tattaaatat
     2281 aaatagcgtt tgttaattac atgatttaaa acgtaaatca gcaaaaactt gtttttgcgt
     2341 agtgtgtggt gcttttggtg gtgagaacca ccaacctgtt gagccttttt gtggagtggg
     2401 ttaaattatt tacggataaa gtcaccagag gtggaaaaat gaaaaaatgg atgttagcaa
     2461 tctgcctgat gtttataaat gggatctgcg aagccgccga ttgctttgat cttgcaggtc
     2521 gggattacaa aatagacccg gatttactga gagcgatatc atggaaagaa tcccgttacc
     2581 gggttaatgc catcggtatt aatccggtaa cgggatatgg cagcggactg atgcaggtag
     2641 attcccagca ttttaacgaa ctggcccgct atggaattaa gccggaacat ctgacaacag
     2701 atccctgtat gaacatttat accggtgctt attatctggc aatagccttt aaaaaatggg
     2761 gcgtctcctg ggaggccgtt ggtgcataca atgccggatt caggaagacc gaacgccaga
     2821 accagagacg tcttgcctac gcatcagagg tttaccggat ttatacctgg ataaagagca
     2881 gtaaaggcat ccgggttccg accacgaaga aatcactttc ccaaattaac agtgtgcaga
     2941 aaaattaata ttgtatacat tgtcaaatac cggttatgcc gcgacagcgg catggaaggc
     3001 cacacacaaa aatatcatat gagtgataat gctgactggc cgaagccccg caaagcggga
     3061 tcccatctta tatatatccg gtaactgcgc agcctcctgc cagaccattt ttcgcgggca
     3121 caatatttac agactaccga aaagccagtt gatgaaattg aggcatatca gacgacacag
     3181 caatgccgca ggatgataat ataagtaaag ccccgaaaaa tttttcgggg ctttacttat
     3241 attatcatga cttccactcc ctgaactgtt cggcaatcat ccataatgct ttgttgattc
     3301 ggatatcacc gtcaatgcct gtaatggctc ttgttctggt atttttaccg gaggcacttc
     3361 gccccgataa tccacctttt atcatattct cctgaacccg ctgccaggtt gtccagagat
     3421 cgttctgttt atcctcccaa cggcggggag taattatttg ttctggcgtc actggcgttt
     3481 tattttcatc ttcgtacctg gccatcaatg cagctctgcc aaataaatgt tgttcgtcac
     3541 tgttaagatg tatctctttc attgtctcca tattttcagt gactttatca aagacaccga
     3601 gcacttcata cgctccctca ataacctgcc cgacaatatc acctttatgt ggaacgcgga
     3661 tttcgccaaa attattccca cacaccaggc catttgtgca gacaaaacga aaaattcctg
     3721 ggatcatctg ataactggat gaaccatcat gtgaattaag caggataatt tcaggaactt
     3781 ctttcccgtt aatatgccct tccctgcgaa gacgtaacat atgtttactg tattcgcggc
     3841 gtcccaaatc acgaacccga ctctgacagg caaagaatgg ctggaaacct tcatcacgta
     3901 acttattgat gatattgatg gttgggatat atgtataacg ctcactccgg gattcatgtt
     3961 tatcaccaga aaagacagat ggaacaaact gcattaattc gtcatccgtt aacgggcgct
     4021 cacggcgaat agaattatat cgaccaaaac gggaagctaa tcgcataaca gtaactcctt
     4081 tatgaaggca taaaattaat aaaagttttt tcgcttaata tcatcccccc tcggttatcg
     4141 ttaacaaaaa gacaacaatt ccgaggggaa ggaccatcaa atcaggtcag ttcgggactg
     4201 accaacctgc cgagtgaaaa gcagtaacag cgcgatcaac tcatcagcgt tttttctgac
     4261 aagaggagta tgattctcca ggctatccag taagtcatgc agctcattat tgacctggtg
     4321 gagcacctta aagagtgcac cagaatcccg gcagctctga tatgcaagat ttgcctgaac
     4381 agcacgattc tgcatgatcc cgatttgtgc gattgcgtct ttctgctgat cgcctgttgg
     4441 ctgataagtg gtgttaaaag aaaatgcgct cataattcct cctgtat
//
LOCUS       DAEEKQ010000246         4481 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.249, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000246 DAEEKQ010000000
VERSION     DAEEKQ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4481
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.249"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(7..1068)
                     /locus_tag="KLK57_003987"
     CDS             complement(7..1068)
                     /locus_tag="KLK57_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HBE0894219.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWQCLWCLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(1065..2462)
                     /locus_tag="KLK57_003988"
     CDS             complement(1065..2462)
                     /locus_tag="KLK57_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HBE0894220.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDTWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTVDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            2617..3615
                     /locus_tag="KLK57_003989"
     CDS             2617..3615
                     /locus_tag="KLK57_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0894221.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     ELIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(3726..>4481)
                     /gene="ompG"
                     /locus_tag="KLK57_003990"
     CDS             complement(3726..>4481)
                     /gene="ompG"
                     /locus_tag="KLK57_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HBE0894222.1"
                     /translation="AEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEV
                     HYQFLENDDFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGW
                     LSMYKFANDLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFS
                     TQEIRAYLPLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNG
                     LSVSLEYAFEWQDHDEGDSDKFHYAGVGVNYSF"
BASE COUNT         1092 a         1228 c         1098 g         1063 t
ORIGIN      
        1 attgcattat ttttcgctgg gtgttttgcc tttttgcagt gtttctttca cctgaccgat
       61 aagctgacga cggaaatccc cgaggcgagg tttgtcatcg tcaatccacg gcagcgggcg
      121 gcaaagctcc atagctttaa tcccgagtcg tgccgtcaga agtcctgcac caatcccctg
      181 agctgcgcgg gtagacaaac gagcagcgag atcttgcgac atccagtcca tccccacttc
      241 gcgcaccagt tcgctggctc cggcaaaagc gatattcagc aataccagct taaacagacg
      301 caaacggctg taatacccca gttcaatgcc atacagcgtg gcgatgcgat taattaaacg
      361 cagattgcgc caggcgataa acgccatatc gaccaacgcc agcgggctga ccgcaatcat
      421 caacgttgat tccgccgccg aacggctgat ttcgcgccgc gcctgggcat ctaaaactgg
      481 ctggaccaaa tgcgcataca ggctgaccac ttcacggtcg ttttgcgttt catggattga
      541 ggcataccag cgttgcagcg ccggatgcga ctgatcaata cccgcctgct gcgccagttt
      601 ttcgcaaaat gcgcggcctt tgcccgtgcc gtggctatgc aataaatcac gcgcttcgtc
      661 gcgttcatgg gcgcgctgtc gcaagcacca taagcactgc cactctgtta ccacagaacc
      721 tacgccagcg ccgataatca atgccccagc ggcacatcca cccagcgcta cccagtcctg
      781 ggtttgccag gcattcattg tccactgtac accctgcccg acaacacttg cgccaaacag
      841 agccagcccg cccatcacca ttttgcgcca caggctgcgt ttcggacgta acgctgcatc
      901 cattaccgct tcaacttgcc cctcttcttc aggcgcttca tcgagcgtgg ccggggcaaa
      961 attttgcgcc tgattttcgt caaaggtctg ctgcgccctg aattttggat tctgatcgac
     1021 ctccagcgga ccgtcgaaat caatacgtgg ttttaacggt tcggtcatcg caatttatct
     1081 cctattaaaa attccagcgc ggcatcaaga cgaatatgcg gcagcggttt gtcgacatcc
     1141 atcacctgcg ggcgaaacgc ttcaaactgg aacccttgct tgtcccagaa cgcctgaccg
     1201 ggcaaacgcg caggaacttc accggggtaa acggtgagag gcgcaccatc gctgagccga
     1261 ttaccgcgca atgccggaat tttctcgccg ttgacatcaa taatgccgct ggtggtcgcc
     1321 tgcaccgagg ccagccccag gcaatccata ctaatccctt caaacgccgc attttgccag
     1381 gcatcctgaa tcagttgttg cagtaatgaa accatattgg cgtgctgatc gaccgttaca
     1441 tggtccgctt tcgttgcggc aaacagcagt ttgtcgataa ccggcgagaa taagcggcga
     1501 aagagcgtgc gctgcccgta gtggaaactt tgcatcagct gcgtcagtgc cagacgcata
     1561 tcattaaatg cctgtggccc actgttgaga ggttgcaggc aatccaccag cacaatctgg
     1621 cggtcaaagc gcagaaaatg attcttatag aaccccttca ccaccttctc gcagtaataa
     1681 ttaaaccgct cgcgcagcat tccggcattg gtgtgcttat cggcctgcgc cagtttggac
     1741 tcgccccagg tatcgacatc cggccacggg aagaattgca gcgcgggcgc acctgccata
     1801 tctcctggca agacaaagcg ccctggctga ataaagtgca gcccctgctg tttacagtgg
     1861 tggagataat cggtccacgc ggcggcaatg tctgccagcc ggttttcgtc ggcaggtgct
     1921 agcgggtcca gcccttcgca catcattcgc catttgaccg accattcgcc gcgctgacca
     1981 ttgagtaagc ccgtcatctg gcgtgaccag cttaaatagt cctgcgccag catcggcagg
     2041 tcgagcaacc attcgccggg ataatccaca atctccagat acagcgtgga ggtgtctttg
     2101 aagtggcgta gcagcgaatc gttcgattta aaacgtagcg ccaggcggat ttcactgacg
     2161 ccgcgcgtag gcgtcggcca ggcgggagga tcgccatata actgcgccag tccttcatca
     2221 taggtgaagc gcggaatgcc aaagtcacgc tgaggaatgc gttttacgcc cagcaggcgc
     2281 tcttcacgca ccgcgcttaa cagcggcaag cgtgctccgg cgtgaatatt gagcaactga
     2341 ttgaccatcg cagtgataaa cgctgttttg ccgctgcggc ttagtccggt tacggcgagg
     2401 cgcagatgtc tgtcgacacc ccgattcacc agcgcattaa gttcattttt aagtcgcttc
     2461 attgctgtcc tgtgctgctc tgaagaccag aaaaaaatca ttcgtaagga tatgaatgtg
     2521 ggggccaggc cattgattat caactgcccg tgttgtcatc gacgggcgaa ctccataaga
     2581 tgtgcagcat tcacgttcgg acggagttcc tgacctatgt cccctactat ttatgatatt
     2641 gccagggttg caggcgtatc aaaatccacc gtatcacgcg tgctgaataa gcaaactaat
     2701 atctccccgg aagcgcgcga aaaagtgtta cgggccattg aagaattaca gtatcagcca
     2761 aacaagctag cccgcgcgct gacctcttcg ggttttgatg ccattatggt gatttctacc
     2821 cgttcgacca aaactacggc gggtaatccg tttttctctg aagttttgca tgccatcacc
     2881 gccaaagctg aagaagaagg tttcgacgtg atattgcaga cgtcgcacaa cccggcagaa
     2941 gacttacaaa aatgtgaaag caaaattaag cagaaaatga ttaaaggcat tattatgcta
     3001 agttcgccag cggatgagtc attttttgcc caactcgata aatacgatat tccggtggtg
     3061 gtgattggca aagttgaagg tcaatatgcc catgtttatt ctgtcgatac cgataatttt
     3121 ggcgacagca ttgcgttgac cgatgcatta attgaaagtg ggcatcaaaa tattgcctgc
     3181 ctgcatgctc cgcttgatgt ccatgtttca gtggatcggg taaatggtta taagcagagc
     3241 ctggctgcgc ataatattgc agtgcgtgat gaattgattg ttgacggtgg ttatacccat
     3301 gaaacagcac tgcaagccgc gagagaatta ttaagccagt caccgttgcc tgaggcggtg
     3361 tttgccactg acagtctgaa attaatgagt atttatcgtg cggcagcaga gaaaaatatt
     3421 gctattccgc agcagttagc ggtggtgggt tatagtaatg aaacgctgtc gtttatatta
     3481 acgcctgcac cgggcggcat cgatgttccg acgcaggagt taggtcaaca aagctgcgag
     3541 ttattattcc gcttaatttc tggaaaaccg tcaccacaga atattaccgt tgccacgcat
     3601 atgacgctga aataaatacc agctaccttg cctgaatgcc ggatgcgatg ctagcgcatc
     3661 ttatccggcc tacatttata gatcggataa agtactcgcg ccgcatccgg caatttagcc
     3721 cattatcaga acgagtaatt tacgccgaca cctgcataat ggaatttatc actgtctcct
     3781 tcgtcgtgat cctgccactc aaacgcgtat tccagcgaaa cggaaagtcc gttctggaaa
     3841 tcataaccgt aaaataaacc tacacggtta aaatcatggc cttcacgttc aatatcatcc
     3901 tgccagtccc agttactcca gcgatccagc ccaatgcgcg tatacggcgt caccgagtgg
     3961 ttgccgagcg tcagcggcaa ataggcgcga atttcttgcg tggaaaactc accgttattg
     4021 cggctgtcgt ccatattgaa gccgcgctcg agataatagt tcactcgcaa ggcaaccgtt
     4081 tcgttgaagg tatattgcag acctgtttcc gtttcgacac gggtatcagc gtaaccggta
     4141 gtgttcagat cgttggcaaa tttatacatc gacaaccaac cgttgaaacg taaatcgtca
     4201 gtcagtttca catcccagtc tggcgcgatt ttccagcgct gcatattcgc cgtgtcttta
     4261 cccggttcat caacgtagtg ataaccataa ttacggaaac cgccggtcag gccgaaactg
     4321 aaatcatcgt tttcgaggaa ctgataatgc acctccagct ccgggcgatc aaaccacgtt
     4381 ccacgtttac ccgcgctata atctaccggc ccttcctgat aataggccag agcaattctc
     4441 cacggcccgt tggcggcatt aaaatagact gaaggctccg c
//
LOCUS       DAEEKQ010000247         4411 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.250, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000247 DAEEKQ010000000
VERSION     DAEEKQ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4411)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4411)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4411
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.250"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(173..2122)
                     /locus_tag="KLK57_003991"
     CDS             complement(173..2122)
                     /locus_tag="KLK57_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HBE0894223.1"
                     /translation="MADSFQLKAIITAVDKVSAPLKGMQRQLKGFKKEFASLLLGAAG
                     AGTAVLGALALPVKSAIALESKMADVRKVVDGLDTPEAFKAMTEQVRDLSTELPMSAE
                     GIAEIVAAGGQAGIARDELMQFTDDAVKMGVAFDTTAEESGQMMAQWRTAFKLTQGEV
                     AGLADKINYLGNTGPASAKKISDVVTRIGPLGSVAGVASGEIAAMGATIAGMGVESEI
                     AATGIKNFMLSLTARDSATKSQKKVLRSLRISPKKLAADMQKDARGAMLHVLDSLAKV
                     PKEKQAAVLKALFGKESLGAIAPLLTNLDLLRTNFNRVADAQQYGGSMQKEYAARAAT
                     TENQLLLLQNQINAISSTLGETFLPSLNEGIKEMKPFLEEVRTFVRENPEVVKTIAKT
                     GAALLTMGVAIGTLTRITKIMGSVMNMTPAKGLIALLVGGAYLIIDNWETVGPVVKKV
                     WQEVDQVVRAMGGWEQAVKTIATVSALYIGVKAVASIRAATVAQNQWTTAAGKTGLKL
                     KGLGKISLIGGLLELGMMAQEFEKAHPWLVKNFVADALNSGFGLNDKFDEWGKQFHDF
                     VYDMTGWQMPRGDGYLSPDKRYTPNVSLERNQLLSLASSPATRSELKVTFDNAPPGMR
                     VIDLPKTGDPFMKITHDVGYSPFKR"
     gene            complement(2207..2542)
                     /locus_tag="KLK57_003992"
     CDS             complement(2207..2542)
                     /locus_tag="KLK57_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HBE0894224.1"
                     /translation="MAMNVTEIVLKKPVTAHNEMLHVLELREPTYDEIEALGFPFIIS
                     GEGSIKLDSQVALKYIPLLAGIPRSSAAQMAKLDIFKTSMQILRFFTQSETGSTSGND
                     STMLPGSGN"
     gene            complement(2542..2898)
                     /locus_tag="KLK57_003993"
     CDS             complement(2542..2898)
                     /locus_tag="KLK57_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tube protein"
                     /protein_id="HBE0894225.1"
                     /translation="MAKIAGTCFFKVDGQQLSLTGGIEVPMNTNVRDDIVGMAGDVDY
                     KETWRSPYVKGTFKVPKNFPVDKITTSDQMTITAELANGMVYVLSAAWLHGEANHNAE
                     EGTADLEFHGEEGGYQ"
     gene            complement(2898..4394)
                     /locus_tag="KLK57_003994"
     CDS             complement(2898..4394)
                     /locus_tag="KLK57_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath subtilisin-like
                     domain-containing protein"
                     /protein_id="HBE0894226.1"
                     /translation="MTISFNTVPSNTLVPLFYAEMNNSAANTAVTSAPALLIGHASND
                     AAIEVNSLVLMPSADYARQICGAGSQLARMVEVYRQTDPFGELYVIAVPEARGAAATV
                     RVTVAGEAEESGTLSLYVGRSRVQVPVVNGDDATAVATAIKEAVNGVITLPFTASSDA
                     GVVTLTARHKGLYGNELPVCLNYYGSGGGEILPAGLRVVTEVGAAGSGAPDLTAAVAA
                     MGDEAFDFIGLPFNDAASINMMMTEMNDSSGRWSYARQLYGHGYTAKLGTLSELVGAG
                     DMHNQQHITLAGYEKETQSPVDELVASRLAREAVFIRNDPARPTQTGELVGMLPAPKG
                     KRFIMTEQQTLLSHGVATAYVEGGTLQIQRSVTTYKKNAYGVADNSYLDSETLHTSAY
                     VLRKLKSVITSKYGRHKLANDGTRFGPGQAIVTPAVIKGELLATYRQMERAGIVENYD
                     LFKQYLIVERDADNPNRLNTLFPPDYVNQLRVFAVVNQFRLQYSEESA"
BASE COUNT         1020 a         1315 c          970 g         1106 t
ORIGIN      
        1 aacgttacta ataaatcgtg aaacaatctc ctcatttgtt agtattttcg cctgagcaca
       61 gcaagaggtt aatattgcaa ccgtcattaa aaatggcttt atcacaaaac aaactccatt
      121 gttagcatgt tacgcgataa ttttgcctca tctttcacca caaaaaaaga actcaccttt
      181 taaatggaga atacccaaca tcatgggtga ttttcataaa gggatcgcct gttttcggca
      241 aatcgataac gcgcattcca ggcggcgcgt tgtcaaacgt caccttaagt tcgctgcggg
      301 ttgccggtga agatgcaagg cttaacagct gattcctttc cagtgataca ttcggggtgt
      361 agcgtttatc gggagataaa tacccatcac cacgtggcat ctgccatccg gtcatgtcat
      421 agacaaaatc atggaactgt ttgccccact cgtcgaactt atcattcaga ccaaatccac
      481 tgttcagtgc atcagccaca aaatttttaa caagccaggg atgtgctttt tcaaactcct
      541 gcgccatcat gcccagttca agcaatccac caatcagact gatctttccg aggcccttta
      601 atttcagccc tgtttttcct gccgccgttg tccattgatt ctgggcgact gttgccgcac
      661 gaatacttgc caccgccttc acaccaatat acagggcaga aaccgttgct attgttttta
      721 ctgcctgttc ccatcccccc attgctctga caacctgatc aacctcctgc cagacttttt
      781 tcacaaccgg accgaccgtt tcccagttat caattatgag atatgcacca ccgacgagaa
      841 gcgcaattaa tccctttgcc ggcgtcatat tcatcacgct acccatgatt tttgttatgc
      901 gtgtcaatgt gccaatcgcc acgcccatcg tcagtaatgc cgcaccagtt ttcgcgatgg
      961 tttttacgac ctcaggattt tctctgacaa acgtgcgcac ttcttccaga aaaggcttca
     1021 tctcttttat gccttcattg agtgaaggca ggaaggtttc ccccagcgtg gaagaaatcg
     1081 cattgatctg gttctgcagc agcaacaact gattttctgt cgtcgcggca cgggcggcat
     1141 attctttctg cattgagccg ccatactgct gcgcatccgc cacacgatta aagttggttc
     1201 gcaacaaatc cagattcgtg agcagcggcg caatggcccc cagggattcc ttcccgaaca
     1261 gcgctttcag cacagcagcc tgtttttctt tcggcacttt tgccagagaa tccagtacgt
     1321 gcagcatggc cccgcgggcg tctttctgca tatccgccgc cagttttttc gggctaatgc
     1381 gcaacgagcg gagcactttt ttctgcgatt tggtggcaga atccctcgct gtcagcgaca
     1441 acataaaatt ctttatcccc gtcgcagcaa tttctgactc cacccccatt ccggcaatgg
     1501 ttgcccccat tgcggcaatc tctccggagg ccacacccgc aacactgcct aaagggccaa
     1561 tacgggtcac aacatcagaa atctttttcg cacttgcagg accggtatta ccaagatagt
     1621 taatcttatc cgcaagccct gccacctctc cctgtgtcag tttaaaggca gtgcgccact
     1681 gcgccatcat ctgaccggat tcttctgccg tggtgtcaaa ggccacgccc atcttcacgg
     1741 catcgtcagt aaactgcatc agctcgtcac gggcgatacc agcctgacca cccgccgcca
     1801 caatttcggc gataccttcc gccgacatgg gcagttctgt tgacaggtcg cgcacctgct
     1861 ccgtcattgc cttaaacgct tccggcgtat ccagaccatc caccactttc cggacatccg
     1921 ccatttttga ttcaagggca atggcagatt tgaccgggag cgccagcgcc cccagtactg
     1981 cggttccggc acccgctgcg cccagtaaca ggctggcaaa ctccttttta aaacctttca
     2041 gctggcgctg cattcccttc agcggtgccg ataccttatc cacggcagtg atgatcgcct
     2101 tcagctgaaa actgtcagcc atgcttcatc tcctcgttaa tgcggacggc ctcggcttcc
     2161 agttctgtaa actgcgaaac agccccccgt cgcagctcca gtggattcag tttccagaac
     2221 caggcaacat tgtagagtcg tttccggagg tgcttcccgt ctccgactgg gtaaaaaaac
     2281 gcaggatctg catgctggtc ttaaaaatat ccagttttgc catctgcgcc gccgatgaac
     2341 gcgggatccc cgccagcaac gggatatatt tcagtgccac ctggctgtcc agtttaatac
     2401 tgccttcacc ggaaataatg aaaggaaaac ccagcgcctc gatttcgtca tacgtgggct
     2461 cgcgcaactc cagcacatgc agcatttcgt tatgcgctgt caccggtttt tttaaaacta
     2521 tttctgtaac gttcattgcc attactgata ccctccctct tcgccgtgga attcaagatc
     2581 tgccgtgcct tcttcggcat tatgattagc ctctccgtgc agccatgccg ccgaaagcac
     2641 atacaccatg ccgtttgcca gctcagcggt aatcgtcatc tggtctgagg tggtaatttt
     2701 gtcgaccgga aagtttttgg gcactttaaa cgtgccctta acgtaaggtg accgccaggt
     2761 ctccttgtaa tccacatccc ctgccatgcc gacgatgtca tcccggacat tggtgttcat
     2821 cggcacttca atgccacctg tcagcgataa ctgctgacca tccactttaa aaaaacaggt
     2881 tccggcgatc tttgccatta tgctgactcc tctgaatact gaagacggaa ctggttaacc
     2941 accgcaaaga cacgtaactg attaacataa tccggcggga acagcgtatt cagtcggttc
     3001 gggttatctg catcacgctc aactatcaga tactgtttaa acagatcgta attttccaca
     3061 ataccggcac gctccatctg acgatatgtc gccagaagtt cccctttgat aacggcagga
     3121 gtgacaatcg cctgccccgg accaaaacgg gtaccatcat ttgccagctt gtgacgtccg
     3181 tacttgcttg tgatgaccga tttcagtttg cgaagaacat atgcactggt atgcagagtt
     3241 tcactgtcca gatagctgtt gtctgccacg ccatacgcat tctttttgta ggtggttacg
     3301 gaacgctgga tctgcaacgt gccgccttcc acataagccg tcgccacgcc gtgagataaa
     3361 agggtctgct gctctgtcat gatgaatcgc ttacctttcg gtgccggaag catccccacc
     3421 agctcccccg tctgtgtcgg acgggcagga tcattccgga taaataccgc ttcacgggca
     3481 aggcgactgg caaccagttc atcgacaggc gactgggttt ctttttcgta accggcaagc
     3541 gtgatatgtt gctgattatg catatctccg gcaccaacca gctctgacag cgttcccagt
     3601 tttgcggtat agccatgccc gtataactgg cgcgcataac tccagcgacc gctgctgtca
     3661 ttcatttcgg tcatcatcat attgatggag gcggcatcgt tgaacggcag accgataaag
     3721 tcgaatgcct catcgcccat agcagcaaca gcggcggtaa gatcaggcgc tccgctaccg
     3781 gcagcaccga cttccgtcac cacccgaagc cctgctggca gaatctcacc accaccagaa
     3841 ccataataat tcagacagac cggtaactca ttaccataca gccccttatg acgggcagtc
     3901 agcgtcacca cacctgcatc agatgacgcc gtaaacggca gggtgataac cccgtttacc
     3961 gcttccttaa tcgcggtggc aaccgcagtg gcatcatcgc cattcaccac aggcacctgt
     4021 acacgggagc gcccgacata cagactcagg gtgccgcttt cctctgcttc tccggcaacc
     4081 gtcaccctga ccgtcgccgc cgcccctctg gcttccggta ctgcaataac atacagttca
     4141 ccgaaaggat ctgtctgacg gtagacttcc accatacgcg ccagctggct ccccgcccca
     4201 caaatctggc gggcataatc tgccgatggc atcagcacca ggctgttaac ctcaatggct
     4261 gcatcgttgc tggcatgccc gatcagtaat gcaggcgcgc tggtcaccgc tgtatttgcc
     4321 gcagaattgt tcatctcggc gtagaacaac ggcaccagcg tattcgacgg aacagtatta
     4381 aaacttatcg tcatgatttt ttagccttat c
//
LOCUS       DAEEKQ010000248         4409 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.251, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000248 DAEEKQ010000000
VERSION     DAEEKQ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.251"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(21..1958)
                     /locus_tag="KLK57_003995"
     CDS             complement(21..1958)
                     /locus_tag="KLK57_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HBE0894227.1"
                     /translation="MNIAASSELVSRLSTHRRVVALGDTDFTDVAAVVITAADSRSGI
                     LALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPPFYD
                     TLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGDLLI
                     HEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKSNHH
                     GALIQAGATSVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKAELPRPFRLAI
                     IQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNENDP
                     GIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYPLFA
                     ALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDYPTS
                     VLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVPATI
                     LAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAEVLP
                     SVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAHSAD
                     MTFRVKPESLILTIFFLGLIP"
     gene            2369..3076
                     /locus_tag="KLK57_003996"
     CDS             2369..3076
                     /locus_tag="KLK57_003996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HBE0894228.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLFGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            3182..3257
                     /locus_tag="KLK57_003997"
     tRNA            3182..3257
                     /locus_tag="KLK57_003997"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3215..3217,aa:Phe,seq:gaa)
     gene            3455..>4409
                     /locus_tag="KLK57_003998"
     CDS             3455..>4409
                     /locus_tag="KLK57_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HBE0894229.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGVDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSNKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRIGS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPI"
BASE COUNT         1149 a         1161 c         1057 g         1042 t
ORIGIN      
        1 gatttactgc tacgtcgccc tcagggaatt aaacctaaaa agaaaatcgt caaaattaga
       61 gattccggtt tgacacggaa agtcatatca gcgctatgcg catcctgggg gttcatcacc
      121 actgacggga aactctgctg gcggaacatc gctttttgta ggtctttgac gtcgaaactg
      181 acatacagat cgtgcatctc ctgacacaac tggcgcaggg tgtagtcgcg atagcgcacc
      241 ggatatttgt tataaacgct cggcaacacc tcagccagcg gcgagtcatc ctcaatatgc
      301 tgttcaaatt gcgccagcat cgccaccagt tgtgccagct tctcgtggct ttccgccgga
      361 gttaataaga acagaatgga gttgagatcg cacttctccg gcacaatgcc gttctcacgc
      421 agatagtgcg ccagaatcgt cgccggaacg ccaaagtcgc tatattcgcc ggtttcggca
      481 ttgatacctg gtgtagtgag taacagcttg cacggatcaa caaaatactg atccgcggca
      541 tatccttcaa agccgtgcca cttcgccccc ggctcaaaac tgaaaaaacg gcggtcgctg
      601 gctaacacag atgtcggata atcctgccac aatttgccat caacaacggg cgggataaac
      661 gggcggaaca gcttacagcg cgcaagaata gccttgcgcg actcaatacc caactcaacg
      721 cactcagccc acagccgacg cccactctcc ccttcatgaa ttttggcgtt aacatccagt
      781 gcagcaaaca gcggatagaa agggctggta gaagcatgga gcataaaggc gttattcaac
      841 cgcttatgcg ggcaaaaacg cgcctgtccg cggatatggt tatctttttt atggatctgc
      901 gacgtctgtg agaatcccgc ctgctgtttg tgcaccgact gagtcacaaa gatccccgga
      961 tcgttttcgt taagttctaa tagcagcggc gagctatccg ccatcatcgg gataaactgt
     1021 tcgtaaccga cccacgcgga atcaaacaga atgtaatcac acagatgccc aacggtatcg
     1081 atcacctgac gggcgttata gacagttccg tcataggttc ccagctgaat aatcgccagg
     1141 cgaaacgggc gcggcagctc ggctttttct ggcgcaacgt cgcgaatttg ctggcgcaga
     1201 tactcttcat taaaacagtg cgcatcaata ccgccaatga aaccaaacgg gttgcgtgaa
     1261 gcttccagat agaccgacgt cgcccccgcc tgaatcagcg cgccgtgatg attcgactta
     1321 tggttgttac ggtcgaagag caccagatcg ccacgcgtta acagcgcatt cgtcaccact
     1381 ttattcgctg ccgatgtgcc gttcagcaca aaataggttt tatcggcatg aaagactttg
     1441 gctgcgaatt tctgcgcatc cttcgccgat ccttcatgaa taagcagatc gcccaatttt
     1501 acgtcagcgt tacacatatc ggcgcgaaag acgttctcac caaagaaatc gtaaaaatgg
     1561 cgtccggcag gatgcttttt aaaaaacgca ccatgttgat gtccagggca agcaaaggtg
     1621 ctgttgccca tctcaacgta ctgcgtcagc gtgtcataaa acggtggcag caaattctct
     1681 tcatactgac aggctgcgga ttccagctcc agccactgct gctcgttgcc gttgattacc
     1741 gccgtaacgc ccgcaggtaa ttcaacagca tgttcggaat acaaaaacac cggtagatga
     1801 aaaccggtgc gcttaagcaa cgcaagaatg ccactgcgac tatccgcagc ggtaatgacg
     1861 actgccgcga catccgtaaa atcagtatct cccaacgcca ccacgcggcg atgagtagaa
     1921 agtcgggata ccagttcact actggcggca atattcattg atttcataag cgcaaaaccc
     1981 gtttcgggga agtaagaata ccggacaaag tgtaaaaccc tgccccatga gatatgggtc
     2041 aaactggtca ccagctccga cgaccgccag acatcagtaa aagcagaaac gctctgattt
     2101 tactgttgtc ctgcagtgaa cgtgcgttaa cttcaccgca tgagcagtaa catagaaagg
     2161 gaaacgtttc gcgcgaaacg gcgataagcg agagaatgta aggagatggc gtgcatcggg
     2221 caaactccgt acagagagga gaaaattcgc gcaatcatgg cacctttcgc taaggcgtgc
     2281 aagccaggac tttgcgacca gacaagccat caacaaggca aaaagttcat aataagaaaa
     2341 tcaaacaata cagctaacag gaactcttgt ggtcatcggc ccttttatca acgctagtgc
     2401 agtcttactg ggtggcgttc tcggcgcact gctcagccaa cgcttaccgg aacgtatccg
     2461 cgtctccatg acatctattt ttggtctggc atcgctgggg attggtattt tactggttgt
     2521 gaaatgcgcc aaccttccag cgatggtttt agctacgcta tttggcgctc taattggcga
     2581 aatttgtttg ctggagaaag gtgtcaatac agcagtcgcc aaagcacaaa atctgtttcg
     2641 tcactcacgt aagaagccag cgcatgaatc ttttattcag aattatgtcg cgattattgt
     2701 cctgttttgc gccagcggca ccgggatctt cggggcgatg aacgaaggga tgaccggcga
     2761 tccgagtatt ttaatcgcca agtcatttct tgatttcttt acggcgatga tcttcgcctg
     2821 ctcgctgggt attgcggtgt cggtaattag tatcccatta ctgatcatcc agctaacgct
     2881 agcgtgggct gccgcgctga tattaccgct gaccacaccg tcgatgatgg cagacttcag
     2941 cgccgtaggc ggtttattgc tgctggcaac cggattacgc atctgtggca ttaaaatgtt
     3001 cccggtggtc aacatgcttc cagcactcct tctggcaatg ccgctttccg ccgcctggac
     3061 cgcctggttt gcctgacaat gcgtgcaata gcggcaaagt gatgatagat tgtgcagtct
     3121 gcagtaaatt gaagaaattt gattgacgag acgaggcgaa tcaggtttaa tgcgccccgt
     3181 tgcccggata gctcagtcgg tagagcaggg gattgaaaat ccccgtgtcc ttggttcgat
     3241 tccgagtccg ggcaccaaat tcatatcaac ggacctccac ggaggtccgt ttttcgtttc
     3301 agaacgccac gatttaagcg ttctgcctac aaatcaattc taccgaactc aaccagattc
     3361 cccccacatc aaccccattg tgtgggtata attgcgggca taccccagtt cgacagaatt
     3421 tgtaccctct ttaacgcatt cagaaggctg aactatggca ctgactgacg caaaaatccg
     3481 ggctgcaaag cccactgaca aggcttataa actcactgac ggggctggca tgttcctgct
     3541 ggtacatcct aatggctccc gttactggcg tctccgttat cgtattctgg gtaaggagaa
     3601 gactctggca cttggtgtgt atccagaagt ttctctctcc gaagctcgta caaaacggga
     3661 tgaggcccga aaactgattt cggagggggt tgacccttgc gaacagaaaa gagctaaaaa
     3721 agtagtccct gatttacagc tctcttttga acatattgcg cgacgctggc atgccagtaa
     3781 taaacaatgg gcacaatcac acagcaataa agtactcaaa agccttgaga ctcacgtttt
     3841 cccctttatc ggcaaccggg atatcacaac actcaatacc ccggatctgc ttatccctgt
     3901 tcgtgctgca gaagctaaac aaatttatga aatcgccagt cgtctgcagc aaagaatatc
     3961 tgccgtaatg cgttatgccg tacagtctgg catcatcaga tataatcctg ctctggatat
     4021 ggctggcgca ttgactacgg taaaacgcca gcatcgcccc gctcttgatc tttctcgcct
     4081 gcctgaactt ttgtcgcgta ttggcagtta caaagggcaa cctgtcaccc agcttgccgt
     4141 tatgctgaat ttactggttt ttattcgttc cagtgaactc agatacgccc gctggtctga
     4201 aattgatatt gacaatgcca tgtggactat tccagccgaa cgcgaacccc tgccaggcgt
     4261 aaaattctca caccggggct ccaagatgcg aacaccacat cttgtgccac tcagcaaaca
     4321 ggctgtagcc atactgacag aactacagac atgggctggt gaaaatggtc tgatctttac
     4381 gggagcacat gacccgcgta aaccaatca
//
LOCUS       DAEEKQ010000249         4401 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.252, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000249 DAEEKQ010000000
VERSION     DAEEKQ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4401
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.252"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..590)
                     /locus_tag="KLK57_003999"
     CDS             complement(<1..590)
                     /locus_tag="KLK57_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HBE0894230.1"
                     /translation="MADSQFYRPGLPQLISMIRSDLLTRFEQDTLLRRMDAEVYARVQ
                     AAAVHTLYGYIDYLARNLLPDVCDEDWLYRHARIKRCPRKEAVAARGFVRWDGVEGTP
                     VLPAGTQIQRDNQVTFTTTATVTAADGLLRVPVVADEPGSAGNTDDGIAMQLGTPVSG
                     LPSTGYADTITDGEDIENLEIWRARVMERYYYIPQGG"
     gene            complement(583..993)
                     /locus_tag="KLK57_004000"
     CDS             complement(583..993)
                     /locus_tag="KLK57_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000973352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HBE0894231.1"
                     /translation="MMINVNGRPVSTGASIDLLTRAVIISLFTWRRAGRDDAPRIFGW
                     WGDTWPAVQNDRTGSRLYLLRRSKLTNKTPQLARDYVREALAWMVEDGVASRLDINAV
                     RTGTDSLALAITIYQRDGNIHNIIFDDIWSELNG"
     gene            complement(999..1532)
                     /locus_tag="KLK57_004001"
     CDS             complement(999..1532)
                     /locus_tag="KLK57_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015967647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HBE0894232.1"
                     /translation="MNGFSLRNLISRAVITAVDSARKCQSVGLKMIAGDQKQHVEHLE
                     PYGFTSAAQNGAEGVALFPAGDRSHGVVVVVADRRYRLKGLKRGEVALYDDLGQSVVL
                     TRSGIVVDGAGKAIIFKNAPKARFEMPVESTADITDNCDSGGLSMQQMRQAYNAHKHT
                     ENGDGGGITDTPDQPMG"
     gene            complement(1510..2592)
                     /locus_tag="KLK57_004002"
     CDS             complement(1510..2592)
                     /locus_tag="KLK57_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate protein"
                     /protein_id="HBE0894233.1"
                     /translation="MNDTVLLRVSGREWGGWTSVRISAGINRIARDFNVAITTRWPGS
                     RDYQPRIKNGELVEVLIGDEPVLTGYVEALPLRYDASSVSMGIVGRSKTADLVDCSAL
                     PLQQSGKNLLRIVSELAAPFGITVVDAGVPQTAVIDAQPEHGETVADCLNRLLGQVQT
                     LAYDDECGRLVLGKPGTGKAATALVLVLGENILSCDTERSIRERFSEYQVSGQRPGND
                     DDFGEATIAAIRQTIQDSGVTRYRPLLIQQSGTATTATCKARCEFEARQRAALTRETT
                     YTVQGWRQGSGALWRPGLSVIVFDPLNNFDNDELVIAEVTYNQDDRGTTTELRVGPAD
                     AYLPEPVTARKKKMLRRISDERVFSS"
     gene            complement(2589..3980)
                     /locus_tag="KLK57_004003"
     CDS             complement(2589..3980)
                     /locus_tag="KLK57_004003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000347288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA circularization N-terminal domain-containing
                     protein"
                     /protein_id="HBE0894234.1"
                     /translation="MDLPSLPSLSSLFSASSGINWRDNLYDASFRGVPFSVESDEGSF
                     GRRVQVHEYPNRDKPYTEDLGRATRRLTINAYLVGDDYAEQRDRLITAIETAGPGTLI
                     HPQFGEMQGCIDGQVTVSHSGTEGRMCRVSFQFVESGELSFPVAGAATARKLEESSVF
                     LDELIEDMFGDFDLAGIPDFIQNDVIARTTDMLGTVQTAFKMVNSAVSAGARLLQGDL
                     SVILMPPSVASDFVHTLQDTWRAGTRLVDNTQDLVQSITTMSGITLDPGLAPRAVWPT
                     DSASVIRQKQQTNLVAAVIRTTAISEATRAVTSLPQPGSLVKNQQAVVAVGGSTERQS
                     DIIHVSHPALDSVAASTGQDERAQPPTRENLTIIRKSLNAAIEQELRRTADDRLFFQL
                     TSLRTELNRDIQARLVQTEETAERTPAEVLPALVLAASWYDDASRETDILDRNAISHP
                     GFVPVRALRVPVR"
     gene            complement(4027..4233)
                     /locus_tag="KLK57_004004"
     CDS             complement(4027..4233)
                     /locus_tag="KLK57_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894235.1"
                     /translation="MTFLIPIYKDDDFDSDTVGFTFAFKMPRGQFFVDVKENGNIRAG
                     VNVNGESGVTYENCKLNMKDINDD"
BASE COUNT         1054 a         1282 c         1040 g         1025 t
ORIGIN      
        1 cccccctgtg gaatgtagta ataacgttcc ataacgcggg cacgccatat ttccagattt
       61 tcaatatctt caccgtctgt aatggtgtca gcgtaccctg tggacggcag accactgacg
      121 ggtgttccca gttgcatggc aataccatca tccgtattcc ccgccgatcc cggttcgtct
      181 gccacaacag gcacccggag aagaccatcg gctgcggtca ccgttgccgt cgtggtaaag
      241 gtcacctgat tatcacgctg gatctgcgtt cccgctggca ataccggcgt cccctctacg
      301 ccatcccagc gcacaaatcc ccgggctgcc accgcttctt ttcgcgggca gcgtttgatt
      361 ctggcgtgcc ggtacagcca gtcttcatca cacacgtccg gtaacagatt tctggcaaga
      421 taatcgatat acccgtacaa cgtgtgtacg gctgcagcct gtacacgggc atacacttcc
      481 gcatccatac gacgaagcag cgtatcctgc tcaaagcggg ttaataaatc gctccggatc
      541 atagaaataa gttgcgggag gccggggcga taaaactgac tgtcagccat tcagttcact
      601 ccagatatca tcaaaaataa tgttgtgaat attgccgtca cgctggtaaa tggtaatggc
      661 aagtgccagc gagtctgtcc cggtccggac agcgttaata tcaagacggg aagcaacacc
      721 atcctccacc atccacgcca gcgcctcacg gacataatct ctggcaagct gcggggtttt
      781 atttgtcagc ttgctgcgtc gcaacagata caaacgcgac cccgtgcgat cattctgaac
      841 cgcaggccag gtatcccccc accatccaaa tatccgtggt gcatcatcac gcccggcacg
      901 ccgccatgta aaaagcgaaa taatcacagc acgcgtcaga aggtcgatcg aagccccggt
      961 cgacacgggt cgcccgttaa cattaatcat catgattttc agcccatcgg ttgatccggc
     1021 gtgtcagtga tcccgccacc atcaccattt tcggtatgtt tgtgggcatt gtaggcctgc
     1081 cgcatttgct gcatgctgag tccgccactg tcgcaattgt cagtaatatc ggcggtggat
     1141 tcgactggca tttcaaagcg cgctttaggc gcgtttttaa aaattatggc cttcccggcc
     1201 ccgtccacca caataccgga acgggtcagg acaacggact gtcccagatc gtcataaagc
     1261 gccacttccc cacgtttcag tcctttcagc cggtaacgtc tgtcagccac gaccacaacc
     1321 acaccatgag aacgatcgcc cgccggaaat aaagcaacgc cctcagcacc gttctgtgcg
     1381 gcagatgtaa aaccataagg ttcaaggtgc tcaacgtgtt gtttctgatc tccggctatc
     1441 attttcaacc ctacagactg acactttctg gcggaatcca ccgccgtgat gacagcccgg
     1501 gaaatcagat tacgaagaga aaacccgttc atcagaaatc ctcctcaaca tttttttctt
     1561 cctggcggta acaggctcgg ggagataagc atctgccggg ccaacccgta attcagtcgt
     1621 cgtgccccgg tcgtcctggt tataggtaac ttctgcgatc accagttcat cattatcaaa
     1681 attattcagc gggtcgaaaa cgatgacaga taaccccgga cgccataacg cgccactgcc
     1741 ctgccgccag ccctgaaccg tatatgttgt ctcacgggta agcgcagccc gttggcgcgc
     1801 ttcaaattca caacgggcct tacaggttgc tgtcgttgct gtgcctgact gctgaatcaa
     1861 caaagggcga taacgggtca cgccactgtc ctgaatggtc tgacgtattg cggcaatggt
     1921 ggcctcacca aaatcatcgt cgttgcccgg gcgctgccca ctgacctgat attcagaaaa
     1981 ccgctctctg atgcttcttt ccgtgtcaca ggaaagaata ttctctccca gcaccagcac
     2041 cagcgccgtc gccgctttac ctgttccggg ttttcccaga accagtcgcc cgcattcatc
     2101 gtcataagcc agcgtctgaa cctgccccag taaccggtta agacaatcgg caacggtttc
     2161 gccgtgttcc ggctgggcat caatcaccgc tgtctgcggc acgccagcat caacaacggt
     2221 gatgccaaat ggcgcagcca gttcactgac tattctgagc aggttttttc cgctctgctg
     2281 gagtggcaaa gcagagcagt caaccagatc ggctgttttg cttcgcccga caattcccat
     2341 gctgacgctg ctggcgtcat aacgaagcgg tagtgcctcc acatatccgg tgagcacagg
     2401 ctcatccccg ataagcactt caaccagctc accatttttt atccggggtt gataatcccg
     2461 gcttccgggc cagcgggtgg taatggcaac attaaaatcc cgggcaatac ggttaatgcc
     2521 cgcactgata cggacggatg tccagccgcc ccattcgcga ccggaaaccc gaagtaaaac
     2581 ggtatcattc atctgacggg tacccttaat gccctgaccg gaacaaagcc cggatgagag
     2641 atggcatttc gatccaggat atcagtttca cgggacgcat cgtcgtacca tgacgcagcc
     2701 agaacaagcg caggcagaac ttccgctggc gttcgctctg cggtttcctc cgtctgaacc
     2761 agacgcgcct gaatatcccg gttcagttct gtacgtaatg acgtcagctg aaaaaacagc
     2821 ctgtcatccg ccgtacgcct gagctcctgt tcaatcgccg cgttcagcga tttgcgtatg
     2881 atggtgagat tttcccgcgt gggtggttgc gctctctcat cctgtcctgt gctggctgcc
     2941 acgctgtcaa gtgccggatg agaaacgtga ataatatcgg actgacgttc agtggaacca
     3001 ccaacagcca caaccgcctg ctgatttttc accaggcttc cgggttgtgg cagtgaagtg
     3061 accgcccttg tggcttcgct gattgccgtc gtccggatga cagcagccac aaggtttgtc
     3121 tgctgttttt gcctgataac cgatgcggaa tctgtgggcc acaccgcacg cggtgccagt
     3181 cctgggtcca gcgtaatgcc ggacattgtc gttatggact gcaccagatc ctgtgtgtta
     3241 tccaccagcc gggttccggc ccgccaggta tcctgcaacg tatgcacaaa atcactggca
     3301 accgacggcg gcatcagaat gacggataaa tcgccctgca gtagtctcgc tccggcagaa
     3361 acagccgaat taaccatttt gaaagctgtc tgaacggttc ccagcatatc ggtcgtccgg
     3421 gcaataacat cgttctgaat aaagtccggt attcccgcga gatcaaaatc gccgaacatg
     3481 tcttcaatca actcatccag gaatacggac gactcctcca gttttctggc ggttgcagct
     3541 ccggcgaccg gaaatgacag ttccccgctc tcaacaaact gaaatgaaac ccggcacata
     3601 cggccttcag tgccggagtg agaaacggtc acctgcccgt caatacagcc ctgcatttca
     3661 ccgaactgcg gatggatcag cgtccccggc ccggcggttt caatcgctgt aatcagcctg
     3721 tcgcgctgct ctgcgtaatc atcaccaacg agatacgcat taatcgtcag ccgtcgcgtg
     3781 gcacgcccga gatcttccgt gtacggtttg tcacggtttg ggtattcatg gacctgaacg
     3841 cggcgtccga acgaaccctc gtcgctctcc accgaaaacg gaacgccacg aaatgaagcg
     3901 tcgtaaagat tatcgcgcca attgatacct gaagatgcag aaaagagtga agaaagagaa
     3961 ggtaaagaag gtaagtccat actgtctcca ctataaaaaa accgccaatt tctggcggct
     4021 tatatattaa tcatcattaa tatctttcat attcaatttg caattttcgt aggtcactcc
     4081 cgactcgcca ttaacattaa ctccagctct gatattcccg ttttctttta catctacaaa
     4141 aaactggcct cttggcatct tgaacgcaaa tgtaaaacca acagtatctg aatcaaaatc
     4201 atcatcttta tatattggga tcaaaaatgt catcgtcgct ggtgatgcat tgtttgcaaa
     4261 atcaaataca cctatacttt tgccgaattc ataagaagct tttctcaata taatcgcacc
     4321 gctggcagaa ggggatgggc acgatatcct cagttgtctg aatccatctg tatccaagtc
     4381 aacgtcatta tctgcagatg t
//
LOCUS       DAEEKQ010000250         4399 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.253, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000250 DAEEKQ010000000
VERSION     DAEEKQ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4399
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.253"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..258)
                     /locus_tag="KLK57_004005"
     CDS             complement(<1..258)
                     /locus_tag="KLK57_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894236.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPAPLPFILSRTYSSYRTKTP"
     gene            487..1095
                     /locus_tag="KLK57_004006"
     CDS             487..1095
                     /locus_tag="KLK57_004006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HBE0894237.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            1193..2584
                     /gene="selA"
                     /locus_tag="KLK57_004007"
     CDS             1193..2584
                     /gene="selA"
                     /locus_tag="KLK57_004007"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HBE0894238.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRDSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPMIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2581..>4399
                     /gene="selB"
                     /locus_tag="KLK57_004008"
     CDS             2581..>4399
                     /gene="selB"
                     /locus_tag="KLK57_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HBE0894239.1"
                     /translation="MIIATAGHVDHGKTTLLQVITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPSFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSVRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKIRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
BASE COUNT          972 a         1129 c         1312 g          986 t
ORIGIN      
        1 gggcgttttt gtccggtaac tgctgtaggt gcgggagagg atgaacggca gcggggcggg
       61 cagggcgatg tcggtttcac cgggaaggac ctttgcaccg agcaggggat tgaccggatg
      121 gccggacgtc actccgccgg ggcacaccga acaggccaca ccggtggggg caccgatgcg
      181 caccccggct gaaccctgaa caatgctacc gccatactgc gtcatgtcgc cctgacgcgc
      241 tgccggtttt ccgctcatct gctcatcctt gttttatcgg gttatttgtc tgcttccagg
      301 caaaacccta cagatttata agaaagcctt aagtgagata tccatcagat agaaacaatt
      361 aggaaaatat ttatatcacc atgataaata tgtgatttat ttggaagtaa caaaccatca
      421 acattttctg ccctttttcc catcaccagg ccatactgat atttttcctc gcacaaagga
      481 ctcggcatga aactcgtcgg tagctacacc agcccgtttg tacgcaaact ttctattctg
      541 ttgttagaaa agggcataac tttcgaattt attaatgaac tgccctataa cgcggacaac
      601 ggcgtggcgc aatttaaccc gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa
      661 tgctggtttg attcgccgat catcgctgaa tatattgagt taatgaatgt cgctccggcg
      721 atgttgccgc gcgatccgct ggagtcgttg cgggtgcgca aaattgaggc gctggcggat
      781 ggcattatgg atgccgggct ggtatcggtg cgtgaacagg cgcgtccggc ggcgcagcag
      841 tctgaagatg aattgttacg ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa
      901 ggatatctgg tcgatggcac actcaaaacc gatacggtca atctggcgac tatcgccatt
      961 gcctgtgctg tcggatatct caatttccgc cgcgttgcgc caggttggtg tgtcgaccgc
     1021 ccacacttag tcaaactggt cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa
     1081 ccgccaaagg cttgatgcgc gatatgtcct cctgacccat ctcacgttac aatccgtggt
     1141 tatgttaaac gcccttctcc gtgtgagagg gccttgatca gccaggtttc ctatgacaac
     1201 cgaaacgcgt tccctctata gtcaacttcc ggctattgat cgcttattgc gcgatagctc
     1261 cttcctttct ttgcgtgata cttatggtca cacccgcgtg gtggaattgt tgcgtcagat
     1321 gctcgacgaa gcgcgagaag tgattcgtga cagccagacg cttcctgcgt ggtgtgaaaa
     1381 ctgggcgcaa gaagtcgatg cccggttgac gaaagaggcg cagagcgcgc tgcgtccggt
     1441 gatcaacctg acgggaaccg tgctgcatac caaccttgga cgagctttac aggcggaagc
     1501 cgcggtggaa gccgttgcgc aggctatgcg ttcgccagtg accctcgagt acgatctgga
     1561 cgacgccgga cgcggacatc gcgatcgggc gctggcgcaa ctgctgtgcc gtattacggg
     1621 ggcggaagat gcctgtatcg tcaataacaa tgcggcggcg gtgttattga tgttggcggc
     1681 cactgccagc gggaaagagg tggtagtatc tcgcggcgaa ttggtggaga ttggcggcgc
     1741 gtttcgtatt cccgatgtta tgcgtcaggc aggctgcacc ctacacgaag tagggaccac
     1801 caaccgcacg cacgcgaatg attatcgtca ggcggtgaat gaaaataccg cactgttgat
     1861 gaaagtacat accagtaact acagcattca ggggttcacc aaagcgatag atgaagcgga
     1921 actggtggcg ctcggcaaag agctggatgt tcccgtagtg actgatttag gcagtggctc
     1981 gctggtcgat cttagccagt acggtttgcc gaaagagcca atgccgcagg agttgattgc
     2041 ggcgggcgtc agtctggtga gcttctccgg cgacaagttg ttaggcgggc cgcaggcagg
     2101 gattatcgtt ggtaaaaaag agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc
     2161 attacgcgcg gataaaatga ccctcgcggc gctggaagcc acgttgcgtc tttatttaca
     2221 ccctgaagct ctgagtgaaa aattaccgac cctgcgcctg cttacccgca gcgcagaggt
     2281 cattcaaatc caggcacaac gtttacaggc tccccttgcc gcacattacg gcgcggagtt
     2341 tgcggtacag gttatgccat gtctttcgca gattggcagt ggttcgctgc cggttgatcg
     2401 cctgccgagc gcggcattaa cgtttacacc ccatgatgga cgcggtagcc accttgagtc
     2461 attagccgcc cgctggcgtg aattgccagt gccgatgatt ggtcgtattt atgacggacg
     2521 attgtggctg gatttacgct gccttgaaga tgagcaacgg tttttggaga tgttgttgaa
     2581 atgattattg cgactgccgg acacgttgac cacggcaaaa caaccttatt gcaggtgatt
     2641 actggcgtaa atgctgaccg tctgccggaa gaaaaaaagc gcggcatgac catcgatctc
     2701 ggctatgcct actggccgca gccggatggt cgcgtgccta gttttatcga cgtacccggt
     2761 catgaaaagt ttctttccaa catgctggcg ggcgttggtg gtatcgatca cgcgctgttg
     2821 gtggtggcgt gcgatgacgg cgtgatggcg cagacccgtg agcatctggc gattttgcag
     2881 ctgaccggta acccgatgct gacagtggcg ctgaccaaag ccgatcgcgt ggacgaagcg
     2941 cgtgttgatg aggttgaacg ccaggtaaag gaggtgctgc gggaatacgg ttttgctgag
     3001 gcaaaactgt ttatcaccgc agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat
     3061 ctgcttcagt taccaggacg tgaacacgcc agccaacata gtgtccgcct cgcgatcgac
     3121 cgcgcattta ccgtaaaagg tgccggtctg gtcgtcaccg gtacggcgtt aagcggggaa
     3181 gtgaaggtag gcgattcact ctggctgact ggtgtaaata aaccgatgcg tgtacgtgcg
     3241 ctgcatgcac aaaaccagcc aacagaaacc gcccatgccg ggcagcgtat cgcgcttaac
     3301 atcgcgggtg atgcggaaaa agagcagatt aaccgtggcg actggctgct tgccgatgcg
     3361 ccgccagagc cgttcacacg ggtgattgtc gagcttcaaa cccatacgcc gctgacccag
     3421 tggcagccgc tgcatattca ccacgccgcc agccacgtta cgggacgcgt ttcgctgctg
     3481 gaagataacc ttgccgagct ggtgttcgac acgccgctgt ggctggcgga taacgatcgc
     3541 ttagtgctgc gcgatatctc ggcccgcaac acgctggcgg gggcgcgtgt ggtgatgctt
     3601 aacccgccgc gtcgcggtaa acgtaagccg gaatatctgc aatggctggc gtctcttgca
     3661 cgggcgcaga gcgatgccga tgcgttatct gttcatctgg aacgcggcgc ggttaacctt
     3721 gcggatttcg cctgggcgcg ccagctcaac ggcgaaggga tgcgcgaatt gctgcaacag
     3781 cctggctata ttcaggctgg ttatagcttg ttgaatgcgc cggttgccgc ccgctggcag
     3841 cggaaaattc tcgacacatt agcgacttat catgagcaac atcgcgatga acctggtcct
     3901 gggcgcgaac gtctgcgacg tatggcgttg ccaatggaag atgaagcgct ggtactgttg
     3961 ctgattgaaa agatacgcga aagcggcgac atcctcagtc atcacggttg gctgcatctg
     4021 ccggatcaca aagcgggctt cagcgaagag cagcaggcca tctggcaaaa agcagagccg
     4081 ctgtttggtg acgagccgtg gtgggtgcgt gatctggcaa aagagacggg taccgacgag
     4141 caggcaatgc gcctgacgct acgccaggcg gcgcagcaag gaataattac cgcgatcgtc
     4201 aaagatcgtt attaccgtaa cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc
     4261 gatcaggagt gtggttcaac ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga
     4321 aagctggcaa ttcagattct ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga
     4381 aatgatcatt tattgcgcg
//
LOCUS       DAEEKQ010000251         4375 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.254, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000251 DAEEKQ010000000
VERSION     DAEEKQ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.254"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..675
                     /locus_tag="KLK57_004009"
                     /pseudo
     CDS             1..675
                     /locus_tag="KLK57_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309971.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            682..1428
                     /locus_tag="KLK57_004010"
     CDS             682..1428
                     /locus_tag="KLK57_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE0894240.1"
                     /translation="MKNIATGGVLERIRRLTPPHVTAPFRTVAEWREWQLAEGQKRSE
                     EINRLNRQLRVEKILNRSGIQPLHRKCSFANYQVQNDGQRYALSQAKSIADELMTGCT
                     NFAFSGKPGTGKNHLAAAIGNRLLKDGQTVIVVTVADVMSALHASYDDGQSGEKFLRE
                     LCEVDLLVLDEIGIQRETKNEQVVLHQIVDRRTASMRSVGMLTNLNYEVMKTLLGERV
                     MDRMVMNGGRWVNFNWESWRPNVSHSRVVK"
     gene            1443..1865
                     /locus_tag="KLK57_004011"
     CDS             1443..1865
                     /locus_tag="KLK57_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HBE0894241.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDG
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1923..2279
                     /locus_tag="KLK57_004012"
     CDS             1923..2279
                     /locus_tag="KLK57_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894242.1"
                     /translation="MEIKPEDELSNIVLFPVKEDDPRNQVNFLYEPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
     gene            2448..3113
                     /locus_tag="KLK57_004013"
     CDS             2448..3113
                     /locus_tag="KLK57_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HBE0894243.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASGIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
     gene            3341..3496
                     /gene="hokD"
                     /locus_tag="KLK57_004014"
     CDS             3341..3496
                     /gene="hokD"
                     /locus_tag="KLK57_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE0894244.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     VDYESRK"
     gene            3746..4033
                     /locus_tag="KLK57_004015"
     CDS             3746..4033
                     /locus_tag="KLK57_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894245.1"
                     /translation="MKIEELREIFSENGLYAVRVENGAIVSHCRIRCLQSQQRKSGAV
                     LFYFCNGLLTDGFILREDEFVTSLRVLKEIGFKAGFLLLLKNKLIYNLEQD"
     gene            4072..>4375
                     /locus_tag="KLK57_004016"
     CDS             4072..>4375
                     /locus_tag="KLK57_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBE0894246.1"
                     /translation="MAHIQLVKQTSSGLLLPATPESCDFLHQIKIGEWIHADFKRVCN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERKLVSGFVDYLCESVGREHTPALSEAAE"
BASE COUNT         1181 a          972 c         1200 g         1022 t
ORIGIN      
        1 ttattcgctt ctgggcatgg gcggatcaac aaatgataga cggtaatgca gattgtaacg
       61 ctcgcggcgt tacaaaaagt gcaatagatc gcatcacttt tatgtctggt tttgctgatg
      121 cgttaattca ggttggatgg ctggtcgaaa atgacggagg gctttctcta cctaactttg
      181 aacgtcataa cggaaaaagc tctaaaaaaa acgggcggtt acaaacgagc gagtaacaaa
      241 aatacgcgaa ctgaaacgaa aaggtaacgc tgccagcgtt acacaaacgg atcaaaaagc
      301 gttaccagag gaagaggaag aggaagatct aaatactgat ctccccctcg ccaaaaacga
      361 gcgtctaaaa aattcgagcc ggaggctatt gagctgcccg attggttgcc ggaaacactc
      421 tggcatgagt gggtccggtt cagacaggca ttgcgaaaac cgattcgaac ggagcagggc
      481 gctaacgggg cgatacggga actggaaaaa ttccgtcagc agggttttac acctgagcag
      541 gtgattcgac acagcatcgc caatgaatac cagggcctgt tcgcgccgaa aggtgttcgg
      601 cctgagacgt tgctccgaca ggttaacacc gtctcgttgc cggacagtgc gatcccgcca
      661 ggcttcaggg ggtaacggac catgaaaaat attgcgacag gcggcgttct ggaacgcatc
      721 cgccgactga ccccgccaca tgtaaccgcc ccattcagaa cggtagcgga gtggcgcgag
      781 tggcaacttg ctgaaggcca gaaacgtagc gaggagatca accgcctgaa tcgccagttg
      841 cgggtggaaa aaattctgaa tcgctcaggc atccagccgt tgcaccgtaa atgctcgttt
      901 gcgaattacc aggtgcagaa cgacggccag cgatacgcgt taagccaggc gaaatccatc
      961 gccgatgaac tgatgaccgg gtgtacaaat tttgcgttca gcggaaaacc tggtaccggg
     1021 aagaatcact tagcggcagc tatcgggaat cgcctgctga aagacggtca gacagtgatt
     1081 gtggttaccg tggctgatgt tatgagcgcc ctgcacgcca gctatgacga cgggcagtca
     1141 ggcgaaaaat ttttgcggga gctgtgcgaa gtggatctgc tggttcttga tgaaattggc
     1201 attcagcgcg agacgaaaaa cgagcaggtg gtgctgcacc agattgttga tcgccggaca
     1261 gcgtcgatgc gcagcgtggg gatgctgaca aacctgaact atgaggtgat gaaaacattg
     1321 ctcggcgagc gggtgatgga tcgcatggtc atgaacggcg ggcgctgggt gaattttaac
     1381 tgggagagct ggcgtccgaa tgttagccat tcgagggttg ttaagtagtt tcaggaggat
     1441 ttatggcgaa accttttact cccgaacagc gggaagaact gaagacgcga attgtggaac
     1501 tcgtgcatca ggacggtcgg gtcacgattc ggcagttgtc agatgaaaca ggtatcagtc
     1561 gtgcgtctgt cggtcgctta tgcatagaac tggtcgcaag tggtgatgta tataattctg
     1621 gctacggctt attcccgtct gaacaggctc gcaaggactg gcaaagcgcc cgcaaaaaac
     1681 tctcgagagt aaaggtgagg aaaccggttg ttgttgatcc ggaccttatc tggtcattac
     1741 ctgacggaga aatacgccgc tacgacaggc gcctgaatat aatctgtcgc gagtgccgga
     1801 agagcgaagc tatgcagcgt gtactggctt tctatcaggg taattttcag gaggcgatac
     1861 tgtgaatgaa attagctatc aggcttcaat taccgctggc attcgcatca aaggagagga
     1921 gcatggaaat aaaaccagaa gatgagttaa gcaatatcgt tttatttccg gtaaaagagg
     1981 atgaccctcg taatcaggtt aattttcttt atgagccatc ggaaagacca tattgccatc
     2041 acgcctctgt acgggttgac gaaaaagagc gtcaggtccg ctgtaaaatc tgcggtgcgg
     2101 ttgtggagcc gtttgactgg atgctctctg tggcaaaaag agaaaccaga ctggcagatg
     2161 atgtaaggct attgcgccag gaggaacagg aaaggcggag aaatatagaa aagctgatac
     2221 agattgagcg taacgcgaaa gcgcggatac gcagggcgac aaaatccaga actgaataat
     2281 taaatttagc actgttaaaa atttaatcct taaccggagg gatttctgca ccctcaaatc
     2341 atcaggagac cacccgaaag ggcggggagc agtcacacat ctgtttccga tagccccgtt
     2401 ctaatgctac actctttgat atttttatga ccccaataaa catatttatg acagttgctg
     2461 atttcaaacg gcccaaattg gagctcccaa acggggcaaa caaactacta ctgcactctt
     2521 gctgtgctcc atgttccggt gaggtgatgg aggcgcttca ggcctcggga atcgactaca
     2581 ccatcttttt ctacaacccg aacattcatc ctcagaaaga gtatttaatt cgtaaggatg
     2641 agaatattcg ctttgctgaa caacacggcg tgccgtttat tgatgctgat tacgacacag
     2701 acaactggtt tgaacgagcc aaaggaatgg aatgggagcc cgaacgaggg atccgttgca
     2761 ccatgtgttt tgacatgcgt tttgagcgga cagcgctgta cgccgctgaa aatggtttca
     2821 gtgtgatcag cagttcactg ggcatttcac gctggaaaaa tatgcagcag gttaacgact
     2881 gtgggcggcg agccgtcgcg cattatccgg gcatggtgta ctgggattat aactggcgca
     2941 agcagggcgg ctcgtcccgt atgattgaaa tcagcaagcg cgaaaaattc tatcagcagg
     3001 aatattgtgg ctgtgtgtat tctctgcgcg ataccaatct acaccgcaaa tctcagggac
     3061 gccctcttat caaaattggt caactccact acggtaaaga agagaaggag tgattttatg
     3121 gggcaccttt ctgattgatt tcatattggc gaggtaagta gaatgactgc gggtgcttga
     3181 ggctatctgc ttcaggcatg aacaccaaaa ggcagataga gaaaagcccc agttaacatt
     3241 acgcgtccgg caagacgctt aacattaatc tgaggccaat ttcatgcttt gcacatgtag
     3301 gttagcctct tacatgccga aaggcaagga gaagcaggct atgaagcagc aaaaggcgat
     3361 gttaatcgcc ctgatcgtca tctgtttaac cgtcatagtg acggcactgg taacgaggaa
     3421 agacctctgt gaggtacgaa tccgaaccgg ccagacggag gtcgctgtct tcgtagacta
     3481 cgaatccagg aagtaagagt gaccgggcgg ggagctgatc ccatccccgc ccacctctga
     3541 tgtgtcaggc atcctcaacg cacccgcact taacccgccc atcgctgtga tctctcagcg
     3601 tttcggcggg ttttttgttg tttatttccg gtgaatttga ttcgcgcacc ttcgcagata
     3661 gaatcgactc acttaagtag cgcgcaggga gaagagggat ggaccccgaa caggggagag
     3721 ctatttatct ggaaggattc tgaagatgaa aatcgaagaa ttgcgtgaaa tttttagtga
     3781 aaatggcctc tatgctgtgc gcgttgagaa tggagcgatt gtcagccatt gccgcattag
     3841 atgtttgcaa tctcaacaaa ggaagagcgg tgctgtgtta ttttattttt gtaatggact
     3901 tctgacggac ggttttattt tgcgtgagga cgaatttgtc acatcattac gggttttgaa
     3961 agagattggt tttaaggctg gttttctgct tttgctgaag aataaactca tctacaatct
     4021 tgagcaggat tgaactcctg ctgtgtaaca ccgtgccacc ggagaaagcc gatggcacat
     4081 atacaactgg tcaaacaaac ctcttccgga ttacttctcc cggcgacgcc ggagagttgc
     4141 gattttctgc atcaaatcaa aataggtgag tggatacacg cagactttaa gcgtgtgtgt
     4201 aactacgcat tccacaagcg ttttttcaaa ctcctgcaac tgggtttcga ttactggact
     4261 ccggtcggtg gggcgatcac gcctcgcgaa cgaaaactgg tgtccggttt cgttgattac
     4321 ctgtgtgaat cagtaggccg ggaacatacg ccagctctga gcgaagccgc agagc
//
LOCUS       DAEEKQ010000252         4344 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.255, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000252 DAEEKQ010000000
VERSION     DAEEKQ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4344
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.255"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..949
                     /locus_tag="KLK57_004017"
     CDS             <1..949
                     /locus_tag="KLK57_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HBE0894247.1"
                     /translation="PLLPVHLSRVANLEFNQFPPTALYHPQARNFLLTEALRGEGAYL
                     KRPDGTRFMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPM
                     IYEKLLGLGIDLTQEPVPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGAN
                     RMASNSLLECLVYGWSAAEDITRRMPDAHGVSTLPPWDESRVENPDERVVIQHNWHEL
                     RLFMWDYVGIVRTTKRLERALRRINMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIV
                     RCAMMRKESRGLHFTLDYPELLTHSGPSILPPGNHYINR"
     gene            complement(934..1671)
                     /gene="trmN"
                     /locus_tag="KLK57_004018"
     CDS             complement(934..1671)
                     /gene="trmN"
                     /locus_tag="KLK57_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HBE0894248.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWLTQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQVGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            1803..3137
                     /gene="srmB"
                     /locus_tag="KLK57_004019"
     CDS             1803..3137
                     /gene="srmB"
                     /locus_tag="KLK57_004019"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HBE0894249.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAIQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(3346..4227)
                     /locus_tag="KLK57_004020"
     CDS             complement(3346..4227)
                     /locus_tag="KLK57_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0894250.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIVDVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVAKELECGELIELPFGEQSQTITAMCAHHAGKAVS
                     PAMHTFIQCVEESFVAG"
BASE COUNT         1106 a         1193 c         1079 g          966 t
ORIGIN      
        1 tccgctattg ccagtacacc tcagtcgggt tgccaatctc gaatttaatc agttcccccc
       61 taccgcactg tatcacccac aggcacgcaa tttcctgtta acggaagcac tgcgcggcga
      121 aggcgcttat ctcaagcgcc cggatggcac gcgttttatg cccgattttg atgagcgcgg
      181 cgaactggcc ccgcgcgata ttgtcgcccg cgccattgac catgaaatga aacgcctcgg
      241 cgcagattgt atgttcctcg acatcagcca taagcccgcc gattttattc gccagcattt
      301 cccgatgatt tatgaaaaat tgctcgggct ggggattgat ctcacacaag aaccggtacc
      361 gattgtgcct gctgcacact atacctgcgg tggtgtaatg gttgatgatc atgggcgtac
      421 ggacgtcgag ggcttgtatg ccattggcga ggtgagttat accggcttac acggcgctaa
      481 ccgcatggcc tcgaattcat tgctggagtg tctggtctat ggctggtcgg cggcagaaga
      541 tatcaccaga cgtatgcctg atgcccacgg cgtcagtacg ttaccaccgt gggatgaaag
      601 ccgcgttgag aaccctgacg aacgggtagt aattcagcat aactggcacg agctacggct
      661 gtttatgtgg gattacgtcg gcattgtgcg cacaacaaaa cgtcttgagc gcgccctgcg
      721 gcgtatcaat atgttgcaac aggaaataga cgaatattac gcccacttcc gcgtgtcgaa
      781 taatttgctg gagctgcgta atctggtaca ggttgccgag ttgattgttc gctgtgctat
      841 gatgcgcaag gagagtcgtg gcctgcattt tacgctggat tacccggaac tgctcacgca
      901 ttccggtcca tcgatccttc cccccggcaa tcattacata aacagataaa aagcctgggt
      961 cagcgccgta tacgcttcgg aatagttctg gtctggccca cgaatgacta agcgatcgct
     1021 aaagcattct cccgcctgcg gggagaatgc cagcagcacc cgatgcggca gtcgcgcttc
     1081 gttttccgcc acatccgtcc gcaaacgtaa atgccagccc atgcttaatg ccagttcagt
     1141 aaaaccatta ccaacctgct ctggcagcac cacgcagaaa aatccctctt cggtaatgca
     1201 ctccgccgca caggtcagca gcgatgggtg atcaagcgta gtggtatagc gagcctgttc
     1261 ccgttgaggt gtcgcgcact ctactccctg ctgatagtaa ggtgggttac tgatgattaa
     1321 atcgaagcgt actgtctgct gtgtgagcca ctgctgaata tccgccgtat ggacgttaat
     1381 ccgctctgcc cacggggact ggttgatatt ttcctgcgcc tgcgcggcag cttcactttc
     1441 cagctcaacg gcatcaatta tcacgctgtc actggttcgc tgcgccagca tcagtgccag
     1501 caacccgcta cccgcgccga tatcaaggca acgtttcacc ccagccaccg gtgcccatgc
     1561 gcccagcaaa atgccatccg ttcccacttt catcgcacag cgatcgtgag caacaaaaaa
     1621 ctgtttaaaa gtaaatccat tacgacgaag cacggatgta gactgtgaca tgaaaataaa
     1681 accttgcagg aaaaacggcg atagcaccgg gtgagaacaa tacccgagaa gcgatatcca
     1741 tacaaacaga tgaagattgc agccgtaacg tctataatca gcgccccaca cagaggtaga
     1801 acatgactgt aacgactttt tccgaacttg aactcgacga aagcctgctg gaagccctcc
     1861 aggataaagg tttcactcgc ccgaccgcca ttcaggctgc cgccattccg cctgcgctcg
     1921 atggccgtga tgtactcggt tctgcgccga caggcaccgg taaaacggcg gcgtatctgc
     1981 tgccagcgtt gcagcacctg ctcgatttcc cacgtaagaa atccggtccg ccgcgtattt
     2041 tgatcctcac cccaactcgc gagctggcga tacaggtggc cgatcatgcc cgcgaactgg
     2101 cgaaacatac gcatctggat atcgccacca tcaccggcgg cgtagcctat atgaaccacg
     2161 cggaagtgtt cagcgaaaat caggacatcg tggtcgccac gaccggacgt ctgctgcaat
     2221 acataaaaga agagaacttc gattgccgcg cggttgaaac gctgatcctc gacgaagcag
     2281 accgtatgct ggatatgggc ttcgctcagg atatcgaaca tattgctggc gaaacgcgct
     2341 ggcgtaaaca gaccctgctc ttttcggcaa cgctggaagg cgatgcgatt caggactttg
     2401 ccgagcgtct gctggaagat ccggtggagg tttctgccaa tccgtccacc cgtgagcgca
     2461 aaaaaattca tcagtggtat taccgcgccg atgacctgga acataaaacc gcattgctgg
     2521 tgcatctgtt aaaacagccg gaagcgaccc gctcaattgt gtttgtgcgt aagcgtgaac
     2581 gtgtgcatga gctggcaaac tggctgcgcg aagcgggcat caacaactgc tatctcgaag
     2641 gtgagatggt acaaggcaag cgtaacgaag cgatcaagcg tttgaccgaa ggtcgcgtaa
     2701 acgtactggt tgcaaccgat gttgccgcgc gcggtatcga cattcctgac gtcagccacg
     2761 tctttaactt cgatatgccg cgcagtggcg atacttattt gcaccgtatc ggacgtaccg
     2821 cgcgcgccgg tcgtaaaggc accgcaattt cgctggtgga agcccatgac catttgctgc
     2881 tgggcaaggt tggccgttac attgaagagc cgattaaagc tcgcgttatt gatgagttac
     2941 gcccgaaaac gcgtgcgcca agcgagaagc agaccggcaa gccatcgaag aaagtactgg
     3001 caaaacgcgc cgagaagaaa aaagctaaag agaaagagaa accgcgggtg aaaaaacgcc
     3061 atcgcgacac caaaaatatt ggtaagcgcc gtaaaccaag cggaacgggc gtgccaccgc
     3121 aaacgacaga agagtaatct caatgccggg tttaagcctg gtattaaaag tgctggcaaa
     3181 cgcaaaactg cctgatgcgc tacgcttatc aggcctacgt ggctcatgca atatattgaa
     3241 tttgcacgat cttgtaggcc ggataaggcg ttcacgccgc atccggcatg aataaagcgc
     3301 actttgtcga caagttaaat gccgggttta atcccgacat tatttttatc cagccacaaa
     3361 actctcttca acacactgaa taaaagtgtg catcgccggg ctaaccgctt ttccagcatg
     3421 gtgtgcgcac atggccgtaa tggtctgcga ctgttcgcca aaaggcaatt caattaattc
     3481 tccgcactcc aactcttttg ccaccgcaaa acgcggcagg tagctgactc cgatattcgc
     3541 cgcgacacaa cgcttgatgc tttcgatact tataagctca atggtgtttt ccaccgtgat
     3601 ccgccgctga cgcagcgtgc tctcaaatat ctgccggaag acacattgtg gttcattgat
     3661 aataaagcta caggcgttat gtcttcccgg ctcagtaaaa tcgacatcta caatttgtgg
     3721 tgaagccacc agcaccagcg attgttcacc caactctcgt cggttcaggg catcatcatt
     3781 ccctacgcga taaaagacgc cgacatcggc ctcatcattc agtagcgcat cacggatcac
     3841 gtaacagttc agcgactgca acgataaacg cacttttggt gcgcgttgcc gaaaacgctg
     3901 caacacctgc ggcattcggt aagagagcaa cgtttcgccc gaaacaacgc gtagttcccc
     3961 gtccggatct gactcctttt tggcagcctc acgaagcgta tccatcactc gggtaagttc
     4021 ataaatgtgc ggcagcaact tttttccttc gcgggtaagg cacatccgtc ggccaatttt
     4081 ctcaaataac tggactgaaa actcctgctc aagctgctga atatgaaaag tcaccgtcga
     4141 ttgtgtacag cacaattttt gcgaagctcg caaaaaggaa ccctcttcca ccacggtttt
     4201 aagcgtaata aaacggcgca gatccataac cccaaaccta tcgaaaatat cgaatctaga
     4261 atataaaaac attcattttt ttaaatgttc cgtgtcgggt actgtctacc aaaacagagg
     4321 agataacaag tgacaccgac cctt
//
LOCUS       DAEEKQ010000253         4327 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.256, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000253 DAEEKQ010000000
VERSION     DAEEKQ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4327
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.256"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..581)
                     /locus_tag="KLK57_004021"
     CDS             complement(<1..581)
                     /locus_tag="KLK57_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HBE0894251.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIDLGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADAITVDAMIPN"
     gene            complement(644..1093)
                     /locus_tag="KLK57_004022"
     CDS             complement(644..1093)
                     /locus_tag="KLK57_004022"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HBE0894252.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(1090..4182)
                     /gene="ebgA"
                     /locus_tag="KLK57_004023"
                     /pseudo
     CDS             complement(1090..4182)
                     /gene="ebgA"
                     /locus_tag="KLK57_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026199.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
BASE COUNT         1029 a         1091 c         1167 g         1040 t
ORIGIN      
        1 aagttcggga tcatcgcgtc aacggtgatg gcgtcagcag gctgtacgcc gccaaccagc
       61 gccgtacctg ccagtaaaat gtaggagagc gttaacagca gcatcagcgt tgaagtgacg
      121 gaggtaattg gccccaacat tttcgccccg ttggtggaaa cccaggtgga gaaggcgaac
      181 agcaccatac tgataatggt ggtggcaacc ggcgtcataa tatattcgta tccgaggaag
      241 gcatacgaag cataagcaat aacgcgcggc aacagtgagg tgaaaaagaa caggtttacg
      301 aaccagtagg tataggcagt aataaatgcc caacgtccgc ccagcgaact ttttacccat
      361 gcgtagacac cggcttctga gtttttattt aacgaaacaa attctgcgat gatcagacag
      421 aagggaataa aataaaaaat cgtcgcgagg aaaaacatcg gtgccgaggc cagtccaaga
      481 tcaatattat tattgataac gttgttaaag ctgtaaacgg cggcaaaagt cagcgagagc
      541 aagccgaatt tgccgattgt attacgtttg gtatcagaca taacacatct ccgaaaatga
      601 gtagggtagc ggtggcggca aactcctttt gccgccgtag ttgttatttg ttatggaaat
      661 aaccatcttc gatggtgact ttgagaacca cttttttgac cgcgttattg cagataaacc
      721 gatacgcttc atggatatcg caaataacga tttgcccttc gtggacctca acggtttctc
      781 cgcagccttt taaatattca cggtcagttt catcacgata atattccact acctgtaatg
      841 tctctttcgg cgcatattca attttttgct gcccttgcag gtagtaatgc acttcaaaat
      901 agcgtcgatg cccggtaaat agcgcatcgg tcgcggagtc tgtctccacg cggtaagtca
      961 atgagtcacc gatggagtgg gcgacgccag gctgaatgtt gtcgatattt tcaatcgctt
     1021 caacgcagcg ttgccacttc ttgccagagg cgtaaatctg gcggaactgt tctaagttat
     1081 cgatgatcct cattgcttat tctcgctgtg caaattcgtg gaaaagaacc ctgcgccgaa
     1141 ctcatacgac gccaggcttt gcgcggtagc ttctccgcca gaaaccggca gcaacgtaaa
     1201 gccgtagctg aagtcacgga accagacgcg ccaggagtcc agcacctcgc tgccccagga
     1261 gttggagccg aggccaagca gctggtgatc gaggttcagg gtgatgtcat cactgcgctg
     1321 cagctcgtta cagtgctggg cagcgtggat gttttcctag gtatagtgcc aggcgctgaa
     1381 gttaattggg cgctgcggaa ccaccagcag accgttaccg tggcggttag tcagtgccgt
     1441 ccagcggaca tgctgacggt taccgttgtt ctgcgggaag ggatagttct cgaacatggc
     1501 atcgacggtg ctgcgccaga tatcgatgat gttagcctgc tggctgtcgg cgtagttttc
     1561 gcccggtcca cgaccgtaat acgccacctg atcgtattcg ccgttaattc ccatggtgaa
     1621 accgatgcac ggaatgatgt gcggatagtc gccgtaacgc tcgccggaaa gcgccacgtt
     1681 aacctggcca tcggcagcga tgcgccagat gtaggtgcag cgcattccga aatcaaacac
     1741 cggcggggca ataaccgtgc ggctgatgat caacacttca ccctcgctct gttctacggc
     1801 aaagtcgcgc agatgttcct gcatgatctg caaatgattc ggctgccaca acccttcgta
     1861 ctcctgcttg tggttgtcga tcatcggctt gaagaagttg atctttggct cgcgggtcag
     1921 cagcgactcg ccatttacct gccaggatgt cggtttgcca ctcatttttg agaaggtgat
     1981 cgcgaagttg tagccgcgaa cggtgcagct caaacgatcg tcttccagcg tcagcggacg
     2041 cgcattattt ggtgcgaaag gcactggctg cgcggtgttt tccttcagcg ggaactgata
     2101 agtggcgata gaatgcccgg cttcgctgta gcgggtgcgg gaatctttgg tcaccgtaat
     2161 gttgaggaac gcttcgcggg cgtccagctg cggcagcgtg atctgcaagg gggcttcgct
     2221 gttcggcgca acgtcgcgca gtttaatctg ctgcgtcgcg agcgtttcac cttcggcgcg
     2281 cacctctgcg tgcagggggt agtcatcaag cgtggtaaac cacagtttat tttcgacttt
     2341 caactcgccg cgagtcagat cccgcgcgtg gatttttacc ggcgcgataa cctgtttgta
     2401 ctctttcagg cccggtcccg gcgtctgatc ggaatagatc aaaccatcaa gacagaagtt
     2461 atagttgttg ggatagtcgc cgtagtcgcc gccgaattta taccagacat tgccgtggtc
     2521 gtcctgtgcc tggatcccgt ggtcgcacca ctcccagaca taatgaccct gaatgcaatc
     2581 gtgcttatag aagacgttct ggtactccgt cagcccgccc ggtccgttcc ccatcgcatg
     2641 agcatattca cagatgatgc gcggcttcgg atgcgggtat tcaccaaact cattcatcag
     2701 cggcacgcgg gtgtacatgg tggaaataat atcgaccact tcagcatcgc gatcttcttc
     2761 gtaatgcacc agtcgcgtgt catccagcgc cttcgctgcg tggtacatcg cgcggatgtt
     2821 acagccatag ccggattcat tgcccagcga ccagatgatg atcgacggat ggtttttctg
     2881 cgcgtgaata tggcgaacaa tgcgctcgac gtagactttt tcccactgcg gatcgtcggt
     2941 aatacggctg atatcgccga cattagcaaa gccgtgcgat tcgacgtcgg tttccgccat
     3001 cacaaacagg ccgtagatat cacacagttc gtaaaaacgc ggatcgttcg ggtagtgagc
     3061 ggtacgcacg gagttgatgt tgtgctgctt catcaactgg agatctttct cgacgcgatc
     3121 cattccaacg gcgcggcctt tgcgatgatc gttgtcgtga cggttgacgc cgtgcagcat
     3181 cacataacgg ttattgatcc agaacagacc gtcgcgcact ttgatatcac ggaagccaac
     3241 gcgttgtggt accacttcca gaacgttgcc gttggcgtct ttcagcgtca tgaccagatg
     3301 gtaaagataa ggggattctg ctgaccattg ctgcggctgt tcgacagtaa aagcaaagct
     3361 ggcgctggtc agtttttcaa ttgccaaatg atcaatggcg ctgctgtgca ccacgcgttc
     3421 gccatcaaac agggtatatt ccagcgtcgt gacgacaggg gaggcggcga gattttccag
     3481 caccacttcg caggaaagcg tggcatcgca ataggcttcg tcaaagtcgg tacgcacggt
     3541 gaaatcgtta atatgtgtta gttgttttcc gatcagataa acatcgcgga agatccccgc
     3601 tgaccaccac atatcctggt cttccacgta ggtagagtcc gcccattgca tcacgcgcac
     3661 acacaacagg ttgtcgccgg ttttaaccat cgcgctgatg tcaaactctg cggtcaggcg
     3721 actgcccttg ctgaaaccca catactgacc gttaacgtag acttcaaaat aggtttcgac
     3781 gccgtcaaat ttaatcagcg tctgtttacc ctgccagccg tcgctgaggg tgaaaatacg
     3841 ttgataggca ccggttgggt tatcgctggg aacaaacggc acatcgatgg ggaacggaaa
     3901 accttcgtcg gtatattgca gtttgccgtg accttccatt tgccacatgg cggggacggt
     3961 aatatgcccc cagtcagcca ttaactcaga ggtgaaggct tcaggcactt gcagcgggtg
     4021 gtcaaaaaag tggaaattcc actgaccgct taagggcaga aacaggctgc tggtttcgcg
     4081 ggcaaaggta cgcgcttgcg caacagaacc atatgaaaaa aagtacgcac gtggcgcaag
     4141 tcggttttcg tgggtgagct gaatgttttc ccagcgattc atagggcctc ccggtagaga
     4201 gaaatcggaa ccattcatgg tgatggtagt ttgtgtaatt gctggggagt ttagtaaaat
     4261 ttttactaaa aagtgacagg taaatgcatt tactttaact ggatcacgaa aataaacggc
     4321 agcactg
//
LOCUS       DAEEKQ010000254         4288 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.258, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000254 DAEEKQ010000000
VERSION     DAEEKQ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4288
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.258"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            74..838
                     /locus_tag="KLK57_004024"
     CDS             74..838
                     /locus_tag="KLK57_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS6100 family transposase"
                     /protein_id="HBE0894253.1"
                     /translation="MTDFKWRHFQGDVILWAVRWYCRYPISYRDLEEMLAERGISVDH
                     TTIYRWVQCYAPEMEKRLRWFWRRGFDPSWRLDETYVKVRGKWTYLYRAVDKRGDTID
                     FYLSPTRSAKAAKRFLGKALRGLKHWEKPATLNTDKAPSYGAAITELKREGKLDRETA
                     HRQVKYLNNVIEADHGKLKILIKPVRGFKSIPTAYATIKGFEVMRALRKGQARPWCLQ
                     PGIRGEVRLVERAFGIGPSALTEAMGMLNHHFAAAA"
     gene            complement(1331..1915)
                     /locus_tag="KLK57_004025"
     CDS             complement(1331..1915)
                     /locus_tag="KLK57_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HBE0894254.1"
                     /translation="MPRPKLKSDDEVLEAATVVLKRCGPIEFTLSGVAKEVGLSRAAL
                     IQRFTNRDTLLVRMMERGVEQVRHYLNAIPIGAGPQGLWEFLQVLVRSMNTRNDFSVN
                     YLISWYELQVPELRTLAIQRNRAVVEGIRKRLPPGAPAAAELLLHSVIAGATMQWAVD
                     PDGELADHVLAQIAAILCLMFPEHDDFQLLQAHA"
     gene            complement(1915..3153)
                     /locus_tag="KLK57_004026"
     CDS             complement(1915..3153)
                     /locus_tag="KLK57_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0894255.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RPLFGIVMIFVGIVGANGSVIMPALFVDAGRQVAELGLFSSMMGAGGLLGIAIHASVG
                     ARISAQNWLAVAFCGSAVGSLLLSQLPGVPVLMLLGALVGLLTGSVSPILNAAIYNRT
                     PPELLGRVLGTVSAVMLSASPMVMLAAGAFVDLAGPLPGLVVSAVFAGLVALLSLRLQ
                     FATMAAAATASAPTHTEGEH"
     gene            complement(3150..4064)
                     /locus_tag="KLK57_004027"
     CDS             complement(3150..4064)
                     /locus_tag="KLK57_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBE0894256.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAEAAE"
     gene            complement(4186..>4288)
                     /locus_tag="KLK57_004028"
     CDS             complement(4186..>4288)
                     /locus_tag="KLK57_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HBE0894257.1"
                     /translation="IEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM"
BASE COUNT          893 a         1333 c         1325 g          737 t
ORIGIN      
        1 aatactcgtg tgggctctgt tgcaaaaatc gtgaagcttg agcatgcttg gcggagattg
       61 gacggacgga acgatgacgg atttcaagtg gcgccatttc cagggtgatg tgatcctgtg
      121 ggcggtgcgc tggtattgtc gctatccgat cagctatcgc gaccttgagg aaatgctggc
      181 ggaacgcggc atttcggtcg accatacgac gatctatcgc tgggtccagt gctacgcccc
      241 ggagatggag aagcggctgc gctggttctg gcggcgtggc tttgatccga gctggcgcct
      301 ggatgaaacc tacgtcaagg tgcggggcaa gtggacctac ctgtaccggg cagtcgacaa
      361 gcggggcgac acgatcgatt tctacctgtc gccgacccgc agcgccaagg cagcgaagcg
      421 gttcctgggc aaggccctgc gaggcctgaa gcactgggaa aagcctgcca cgctcaatac
      481 cgacaaagcg ccgagctatg gtgcagcgat caccgaattg aagcgcgaag gaaagctgga
      541 ccgggagacg gcccaccggc aggtgaagta tctcaataac gtgatcgagg ccgatcacgg
      601 aaagctcaag atactgatca agccggtgcg cggtttcaaa tcgatcccca cggcctatgc
      661 cacgatcaag ggattcgaag tcatgcgagc cctgcgcaaa ggacaggctc gcccctggtg
      721 cctgcagccc ggcatcaggg gcgaggtgcg ccttgtggag agagcttttg gcattgggcc
      781 ctcggcgctg acggaggcca tgggcatgct caaccaccat ttcgcagcag ccgcctgatc
      841 ggcgcagagc gacagcctac ctctgactgc cgccaatctt tgcaacagag cccgccgtgc
      901 tagtctgctc ggtgatggtg gagtgaagcc aacccgcaat cgggttatga atctgcatcg
      961 cgattcgcaa tcagctgtct cttgagcatg tcgaactcct gcgatgttat ctcgccgtgc
     1021 tcgtgctgct acaccaactt gtccaactca tccgccacac cgctccccac cacctgcgat
     1081 ggccaggttg tttgctgggc gcattggcgg agtatcgtct cgacccggga cacccacacc
     1141 ggcacaacct tacgggagtg attcactgtc aaagaatcgg cccggtgctc tgacgcaagt
     1201 atcgggatgg tcaccatttg taagccgtag acctgagtgg tgatcaagac ttcgatacca
     1261 ccgaccgtac cggtactaat cgacgacggt cgtgttcgtc gcctgccgca gggactctgc
     1321 acacctccgt ttacgcatgt gcctggagga gttggaaatc gtcgtgttcg ggaaacatta
     1381 aacacaggat ggcagcgatc tgagccagca catgatcagc tagctcacca tccggatcga
     1441 cggcccactg catcgtcgcg ccagcgatga ccgagtgcag gagcaactca gctgccgcag
     1501 gagcacctgg gggcagtcgc ttgcggatcc cctccaccac cgcgcggttc cgctggatcg
     1561 caagcgtgcg tagctccggc acctggagct cgtaccagga gatgagatag ttcaccgaga
     1621 agtcgttgcg agtgttcatg ctccgaacga gcacctgcaa aaattcccag agcccttgcg
     1681 gccctgcgcc tatcggtatc gcattcaggt aatgccgcac ctgctcgacg ccgcgctcca
     1741 tcatcctcac cagcagcgta tcgcggttgg tgaagcgctg gattaacgct gcgcgggaga
     1801 gccccacctc ctttgctact ccgctgagcg tgaactctat gggaccgcaa cgcttcagca
     1861 ctacggtggc ggcctcgagt acctcgtcat cggacttgag cttggggcgg ggcatcagtg
     1921 ttcaccttct gtatgggttg gggcggaggc tgtggctgcc gccgccattg tagcaaattg
     1981 aagacggagc gagagtagag ccacgagccc cgcaaacacg gccgatacaa cgaggccagg
     2041 gagcggacca gcaaggtcga caaacgcgcc ggccgcaagc ataaccatgg gcgaggctga
     2101 cagcatcacc gccgagaccg tgccgagtac ccggccgaga agttctggcg gcgtgcggtt
     2161 gtagatggca gcgttgagaa tgggagagac tgagccggtc agcagtccca cgagcgcgcc
     2221 caacaacatc agcaccggca cgcctggcaa ctgtgaaagc agaagcgagc ccaccgcaga
     2281 gccacaaaat gccaccgcca gccagttctg cgctgatatc cgggcgccga ccgacgcatg
     2341 aatggcaatg ccaaggagac caccagcccc catcattgag gagaacagcc cgagctctgc
     2401 tacttggcgt cctgcatcta caaacagcgc aggcatgatg acgctgccgt tggcgccaac
     2461 gatgcccacg aagatcatca ctataccaaa gagagggcgc agcaggggtt cgctccagag
     2521 aaaagcgacg ccggcgcgca tggagagagt cgccgtcgtg gtcatcgtcc gagcggcacg
     2581 cgcgggaagc acccacgcgc cgagcagacc tgcaaggacg gagcagaacg ccgtcagccc
     2641 gagcgttggc gcagcgccaa gcaggccgat tgcggccccc ccaagggccg ggccacctag
     2701 aatcgcgacg ttcccgatca ccgctttcag tgacgagacg cgctcaacgg agagcccggc
     2761 gacgtggccg agtttgggca gctcactgtc ctgcgcggcc ataccgggtg cgtcgaacgc
     2821 ggcaccgagc accacgcaag cgatcagccc agtgttcgag agggcgccaa cggcatcgag
     2881 cagtgggatg ctcgccatgg ccacgccgcc caccacaccc gagatcaatg cgacgggcgc
     2941 gcgcccgaac cgatcgacga ggccaccacc aacccacgcg ccgatgatgg tcgcgatgac
     3001 gctgctagcg gccgtggcgc ccgcccaggc cgcgctcttt gtatgagaca ggacgaacca
     3061 tggaagcgcg agggccgcca ccgcgttgcc gatccggaag agaaaggtcg ccgcgaacag
     3121 cgtcgcgagc gggctatatc gacgttcgct cattccgctg cttccgctgc ggcgagctgc
     3181 gccttcgccg cagcgaggta ctcttcgtta cccgagtcga gggcgaagag tgcgtaggtg
     3241 accgccccga acgcaaggcg ctccgcgatg tggtgggcga gccgcggcca cacccggcca
     3301 ccggccgctt catacgtgag gaggagcttc gcgagcccct cttcaccaaa gaccataagg
     3361 tgcgcggcca tgtcgatggc agggtcatca acgcgggcct cgctccagtc gatcatcccg
     3421 ctgacgcgct ccgtgttgtc gatgagcaca tggcccacgt agagatcgcc atgcaccacc
     3481 acggagaaat ctggccacga cgaatcgtcg tcgagccagc gctgccaccg gtggaggcgc
     3541 ttgtcgttca ccacgaactc gcgtcggacg cggtcaacgt cgtcggccac cttctgacgg
     3601 gcctgcgtcg gtgtacggat gagcatcccc gcatccacgg cggcggaaat ggggacggca
     3661 tgcagggcgg cgagcgcggt cgcgaagctc tccgcgaaga cctccgagtc ctgcggcacg
     3721 acccagtcgg gcgtggacga accaggctgg atgaccatcg cagtcgagtc ttcgagcatg
     3781 ggataggcaa cgagctcggc gttggccacg cgccagtccg gcaccgcgaa cggcaggcga
     3841 ttcttgagca ttgccagcac ccgcgcctct ggttcgacct tcgcgcttac ctcggctcgg
     3901 cgcgggatgc gcagcaccca ccgacgtcca tcgtcgacgg tggcgatcac gatcctatag
     3961 tcgagcccaa gctcattgac agtcagcggg ccatggagct tgagcccatg tcgggctgca
     4021 agtgcgtaca gttgggaggt atcggcggtc gtgactacgg tcatgattca ctcctgaggg
     4081 cttgacgggt ttagccacct aaatgtaaca gtcacgtcgg ttatattcaa tccggcactg
     4141 ttgcaaagtt agcgatgagg cagccttttg tcttattcaa aggccttaca tttcaaaaac
     4201 tctgcttacc aggcgcattt cgcccagggg atcaccataa taaaatgctg aggcctggcc
     4261 tttgcgtagt gcacgcatca cctcaata
//
LOCUS       DAEEKQ010000255         4237 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.259, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000255 DAEEKQ010000000
VERSION     DAEEKQ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4237
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.259"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            7..261
                     /locus_tag="KLK57_004029"
     CDS             7..261
                     /locus_tag="KLK57_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010891415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EexN family lipoprotein"
                     /protein_id="HBE0894258.1"
                     /translation="MCTLLMSIFILSACGEDTKSSDWWLNHPKEATEKYKECKKSGED
                     SVNCQNVKKVAGIIGRTYGPMLEILKAESAEYDKQHGLNR"
     gene            266..1264
                     /locus_tag="KLK57_004030"
     CDS             266..1264
                     /locus_tag="KLK57_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBE0894259.1"
                     /translation="MSGFFEKFNSEVVSKIEEMTKPLQSQLGQDMLSLLSAGISVYIL
                     WVGYQILAGKRQTPLQDLVWDLSKFAIILTFVSNADGYLTAAMDAIKSMKDGFLGKPV
                     WATLDTMWSTTQLLADKVYAMDQSKYVSVEGGIGSMLVWGGSILIMSVAAVIYMLADV
                     TMQLAILVAPIFIGCYMFGFTRVMFNNWLGILFSSVFTVIFAGVLVKIGTEFQTDMLS
                     QISRDANSTNILTMGAMAFVIGVLIAVFVWKSSSFAQQLAGAGVEGTLQGMALLGIGA
                     AGMAVEKTRNQMKRGTRTAAEWAGEKGEQKRASIGALGMEARRRASLEKARARNSA"
     gene            1261..1974
                     /locus_tag="KLK57_004031"
     CDS             1261..1974
                     /locus_tag="KLK57_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001590713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system protein"
                     /protein_id="HBE0894260.1"
                     /translation="MNKKLIFSVLLCLFSFGSVAGIPVAIDASPEWAVEATRWTERLK
                     QWSETARHYQSQIQAYKDQLATATGIRNIAAFTNELSNLQSELANIYKQGNSYISDFT
                     SNPEGALSSQAKNLFSKYGAFNMCSTGIESSDNLCKARIVSTAASIEQGNEINKQLSS
                     AMSQIQSLSNRIESSKDMKESQDLANALQAQSLKIQAIKMQYDVWDSKNRADAEMLKE
                     QKYAEYRLRQKNAKIPSFE"
     gene            3162..3677
                     /locus_tag="KLK57_004032"
     CDS             3162..3677
                     /locus_tag="KLK57_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication/maintenance protein RepL"
                     /protein_id="HBE0894261.1"
                     /translation="MSSLSLQRYETNPFLELMTIPMKGKRIQVSRLGRDDNVLVNQNT
                     GEKFGTHVTTFKRVDSEQFVKLFTANIGLTFDLSSAGIKAFNVLLWAVQKQAFAKDQV
                     LLDSRALDDFLKSYDSLKLSYATLKRGINELEKAQIIAKTMRKGFYFINPNFVFNGDR
                     IAFTTLIERDC"
     gene            3740..4147
                     /locus_tag="KLK57_004033"
     CDS             3740..4147
                     /locus_tag="KLK57_004033"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894262.1"
                     /translation="MSKNDVFACACRRLLEVVAGEQKWCFVRLLITTTWLEVFMTKHD
                     AIRISQLHQIFYDDDGLIDSEKNVTILYSIGFTVDEIAHFRNSTPNSVASQLLNARVK
                     LGCASVSSLKPMILLRLLLNIKEIRFGFETDCK"
BASE COUNT         1297 a          760 c          966 g         1214 t
ORIGIN      
        1 aaaaaaattt gtacgctctt aatgtctatt ttcattcttt ccgcatgtgg tgaagataca
       61 aaatcatcag attggtggct taatcatccc aaagaagcga cggagaaata taaggagtgt
      121 aaaaagtcag gggaagattc tgttaattgt caaaatgtta aaaaagttgc cggaattatt
      181 ggtagaacct atggccctat gcttgaaata cttaaggctg agtcagctga atatgataag
      241 caacatggat tgaacaggtg ataacatgtc tggtttcttt gaaaagttta atagcgaagt
      301 tgttagtaaa atcgaagaga tgaccaaacc acttcaaagt caattaggtc aggatatgtt
      361 atctcttcta agtgcaggta ttagcgttta tatcctctgg gttggctatc aaatattggc
      421 agggaaacgc cagaccccat tacaagatct cgtctgggat ctatcaaaat ttgccattat
      481 tttaacattc gtaagcaatg ctgatggcta tctgacggca gcaatggatg ctattaaatc
      541 tatgaaggat gggttccttg gtaaacctgt atgggcaacg ctagatacca tgtggagtac
      601 aactcagctt ttggctgaca aagtttatgc tatggatcaa tccaaatatg tgtcagttga
      661 aggtggaatc gggtcaatgt tggtttgggg aggaagtatc ctgattatgt ctgtagcggc
      721 tgttatttac atgcttgccg atgtaacaat gcagttagct attcttgtag ctccgatatt
      781 tatcggttgt tatatgtttg gattcactag agtaatgttt aataactggc ttggtatatt
      841 attttctagt gtatttactg ttatttttgc tggagttctc gtcaaaattg gtaccgagtt
      901 tcaaactgat atgcttagtc agatatcgag agatgctaac tcaacaaata ttttaacaat
      961 gggcgcaatg gcatttgtaa ttggagttct gattgctgtc tttgtctgga aatcatcgag
     1021 tttcgcgcag cagcttgccg gtgcaggtgt tgaaggtact cttcagggaa tggctctttt
     1081 agggattggg gctgctggaa tggccgtaga gaaaacgaga aaccagatga aacgtggaac
     1141 gcgaacagcc gccgaatggg caggtgaaaa aggggagcag aaacgggctt caataggcgc
     1201 attgggaatg gaagcaagaa gacgagcatc cctggaaaaa gcacgtgcga ggaattcagc
     1261 atgaacaaaa aactaatttt ttcagtactt ttgtgcttat tttcatttgg ttctgtcgca
     1321 ggtattccgg ttgctattga tgccagtccg gaatgggctg ttgaagccac aagatggaca
     1381 gaacgactca aacaatggtc tgaaacagcg cggcattatc aaagtcagat tcaggcatat
     1441 aaagaccagt tagccacggc aacaggtata aggaatattg cggcattcac aaatgaacta
     1501 agtaacctac aatcagagct ggcaaacatt tataagcagg ggaacagtta tatttctgat
     1561 tttaccagta atcctgaagg tgcattaagt agtcaggcga aaaatctgtt cagcaagtat
     1621 ggtgctttta atatgtgttc tacaggcatt gaaagttctg ataacctttg taaggccaga
     1681 atagtttcaa cggccgctag cattgaacag ggaaatgaga taaataagca gctatcaagt
     1741 gcaatgtctc agattcagag tttaagtaac cgaattgagt cttcaaagga tatgaaagaa
     1801 tcccaggatt tggctaacgc attgcaggcg cagtcgttaa aaatacaggc aattaaaatg
     1861 cagtatgatg tatgggatag taaaaacagg gctgatgcgg aaatgttgaa agagcaaaag
     1921 tatgctgagt atcgcctgcg tcagaaaaat gcaaagatac cgagtttcga atgatatgat
     1981 aaagctaaat aagctaaaag aatgggtaga taatcattgg tctagattga atagtaccgg
     2041 ttctacaccg ccgccactgt taatctcgct accaatcata gttattgttt taagtgtgga
     2101 gcttacgtta aagctgatta atgctttaat gtattatggt acattgcaat ttatcactga
     2161 taaaattgaa aagaggtttg ttcccagcgg tggtataaaa gaaagactcc tttttacgtt
     2221 aaagctgttt tgtattttac ttctgttatt aatagtaatg cgattttcaa tgaagtaaaa
     2281 aataaaggtc atcgaataga tggccttttc tattctaaat tctaaatata gagcctgcgg
     2341 caggcacagg caaaaacatc gtttttggac aatcatggat aaaatgttaa cattttgacc
     2401 atgattgtct atcctgcatt gctcttttgc cgacctcaaa acccctagcg tgtttttgtt
     2461 tcccgatctc tagaaccacc gagccgccac ggttccggca gcgccttgag cgcaaccgga
     2521 aaccgtatgg ggagaacccc agacccccat cgctgtaaca tcgacggtct acgaccacct
     2581 accaaccgtg tggctacctg ttccatgcta atgggctttg cagggggctg gcgcggttcc
     2641 tggcgacctg aaagcccctg gttaggggta tcggcgcgcc tgacctcctg aaaccctctg
     2701 taagcatttt tcgcgccttc catgccctga cgggcatcta aagcccgttt cgtgcgtctg
     2761 agcgcgtttt aagagctaag acgactaaaa cattaccaac acctgaaaat cgcgttctgg
     2821 tgctctggat ggcccacaga acgcattttc gaacagaaca gcaacggcat ttctgacaac
     2881 atgcgagagg tgatcccgct cgccgcagcc ccagcgaccg aacgtagtga gcgagtgggc
     2941 gaggaagcgg aagagaattg atagccataa ttgagcacct acgctcctta gttaagaact
     3001 aagaagggaa attctcggaa cccgcatgaa tcctagaaag tggtgatcag gatctgtacc
     3061 ttgtaagctc agatccggta ccttgcaggc tcaaaaattg cttgattttg agcttatgag
     3121 gatttattgt gaatctccaa agcccaaaaa aggatgcaat aatgagctcg ctaagcttac
     3181 agagatacga aacaaacccg tttttagagc ttatgacgat cccgatgaaa gggaaacgga
     3241 tacaagtatc tcgtcttggg cgggatgaca atgttttggt taatcaaaac acgggagaaa
     3301 agtttggaac acatgtaacc acttttaagc gtgtagatag tgagcaattt gttaagttgt
     3361 ttactgcgaa tattgggtta acgtttgatt tatcatcggc aggaattaaa gcttttaatg
     3421 ttcttttatg ggctgttcaa aaacaagcct ttgcaaagga tcaggttctt ttggattcaa
     3481 gggctcttga tgacttttta aaaagctatg atagcttaaa acttagttat gctacgttaa
     3541 agagaggaat taacgagtta gaaaaagctc agattatagc aaagacaatg cgtaaaggat
     3601 tttattttat aaaccccaat tttgtattta atggagatcg aatagcattt acaaccctaa
     3661 ttgagaggga ttgttaatcg atagaacaat acaatgcagg atagacaacc cgatgtcaaa
     3721 tgttaacatt tgaatcagat tgtccaaaaa cgatgttttt gcctgtgcct gccgcaggct
     3781 actagaagta gtcgcaggcg aacaaaagtg gtgttttgtt cgcctgctaa tcacaacaac
     3841 ctggctagag gtttttatga ctaaacatga tgctatacgc atttctcaac ttcatcaaat
     3901 tttttacgat gatgatggat taattgattc agaaaagaac gtgactattt tatacagtat
     3961 aggatttaca gttgatgaaa tagctcattt tagaaattca acaccaaact cagttgcgag
     4021 tcagctatta aatgcaaggg ttaaattagg ttgcgcctcg gtttcatctt taaagccaat
     4081 gatcttatta agattgcttt tgaacataaa agagattcgt tttggattcg aaactgattg
     4141 caaataatat agttcaatag gtatttttgc tcttgactag aattacaatt gctgattgta
     4201 tcagcaattg taatgtccat acgaggaatc aaagatg
//
LOCUS       DAEEKQ010000256         4233 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.260, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000256 DAEEKQ010000000
VERSION     DAEEKQ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.260"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..331
                     /locus_tag="KLK57_004034"
     CDS             <1..331
                     /locus_tag="KLK57_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBE0894263.1"
                     /translation="IEAWRTEQEAKPFTFEWNGRIWNAGPNSLGRLYPVVMAAKSDIV
                     RDVMTWGDADNQQVKQSMPELEELAAAMAQAQVERNDEIYRRQRELKEELNSLKDLNS
                     VRNFIVE"
     gene            complement(532..1389)
                     /gene="sitD"
                     /locus_tag="KLK57_004035"
     CDS             complement(532..1389)
                     /gene="sitD"
                     /locus_tag="KLK57_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HBE0894264.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISAKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="KLK57_004036"
     CDS             complement(1386..2243)
                     /gene="sitC"
                     /locus_tag="KLK57_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HBE0894265.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="KLK57_004037"
     CDS             complement(2240..3067)
                     /gene="sitB"
                     /locus_tag="KLK57_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HBE0894266.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNDSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="KLK57_004038"
     CDS             complement(3067..3981)
                     /gene="sitA"
                     /locus_tag="KLK57_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HBE0894267.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
BASE COUNT         1143 a         1040 c         1058 g          992 t
ORIGIN      
        1 aattgaagca tggcgcacag aacaggaggc gaagccgttc acgtttgaat ggaacggtcg
       61 tatctggaat gctggcccca actcactggg ccgcctttat ccggtggtaa tggctgcgaa
      121 atccgacatt gtacgggacg tgatgacgtg gggtgatgcc gataatcagc aggtgaaaca
      181 gtcgatgccg gaactggaag aactggcggc agcaatggct caggcacagg ttgagcgcaa
      241 tgacgagatt tatcgccgtc agcgggagct gaaggaagag ctgaatagtc tgaaggattt
      301 gaattcggtt aggaatttta tcgtggaata acagaagctg cggcacgtcg tatgcaggaa
      361 cgtgccgcgg ttggctggta aactttcgat agtgcgagta ttgaatgatt tctaggcgtt
      421 atcgatttta cgtatttttt gcatgataga attcgtacct cctcccaacg accttccatg
      481 gctttccgga cttctgtagg ctggcggacg atgctgtcct cggatgttgt gctaattggg
      541 tgataacaga tcctgtgtat gggcgtttcc ctttgtgcgt gcgttgatgc tggtaacggt
      601 aaacgtcatg atgaacacga ccgcgaataa gacgacaatc gtcggagctg gtgcgctgtc
      661 gataaaaaag gacagccaga caccagtcat tgacactatt accgatatgc cagtcgccag
      721 taggagcgca atatgaaagc gttgtgtcag gagtactgca atggcgccag gcgcaatgag
      781 cagagaaata gagagaatga tacctactgc tttcagcgtc gccacaatgg tcagggaaac
      841 catgcacagc agcccataat gcagccatct cgtatgcaaa ccgcttacct gcgcctgttg
      901 ataatcaaag ctgaacagca aaaaatcccg ccattttgcg ctaataacaa gtgtaaccag
      961 cccggcaata atcaccgtct ggattatatc gccgatggtt atcccgagca tgtcgccgaa
     1021 aagaatatgg tcaagatgta cgtctggttt gattgcaata tacaagatca gacctgcggc
     1081 aaacatgcca gaaaaaacaa tccccataac tgtatcttgc ttgattcggc tgttgtcttt
     1141 caggtatccc gtcgccaccg cgcagaatac tccggcgacg aatgcccctg tagccagagg
     1201 taaccccaga atccaggtaa gcacgatgcc aggaaaaacc gcatgactca tcgcgtcacc
     1261 catcagtgcc caacctttca ggaccagaaa gaccgacagc agcgcacagg gtatggaaac
     1321 taccagtgaa atcagcagtg cgtgactcat aaaggtgaac tgcagtggct ccagtaataa
     1381 tgccatcatg agcgatcctc cagcgcttta tgcgcacgcc gacgatttgc caaaagtcca
     1441 tgggtgggcg caaatacaaa tgctaaaaga aacagcagtg tctgcgccac cacgatgatt
     1501 ccacctgtcg cgccgtccag aaagtaactg acccacgcgc cgaggaaact ggttacactg
     1561 ccaatagtta cagcaattat gagcaggcgg ggaaaacgat cggttaacaa ccatgctgtt
     1621 gcaccgggag tcaccaccag acagatcacc agaaaggcac caaccgtttg cagcgccgca
     1681 acggtcgaaa ccgacagcag ggtgaagaag aggattttta atctgcccgg atgtaatcca
     1741 atagcacggg cgtgattctc gtcgaaaaag gtcaccataa gatctttcca tttgaaaaaa
     1801 agcacgatga tcgagagaat gccaatgata gtgagttgca ggatatccgc cggatcgatt
     1861 gccagaatat tgcccaggac gatggtctga atgttcacgg atgtcgggtt caatgacacc
     1921 ataaacagcc ccaggccaaa aaaggacgag aagatcaggc cgatgatggc atcttctttt
     1981 aagcgggtgc gttggttaag aaacagcatg ctgcccgctg ccagtccgcc ggaaaagaag
     2041 gctcccagag aaaacggaag ccccagcata tatgcccccg caacacccgg gacaatagag
     2101 tgcgacagcg catcgccaat cagcgaccag ccttttagca tcagatagca tgacaggaaa
     2161 gcgcagagac cgccgaccat tgccgagacc cacatcgcat tgagcatata ttcatagctg
     2221 aagggttcca gtagcacgtt catcttgctt ccctctgcac cgctgacggt cgatgtgcca
     2281 caaaagggcg ttcatcgtca gtaataatgc tttcttccga gtcgttaagt gtgacatggc
     2341 gcagtacgcc gctaaaagcc agctccaggt tctcggctgt aaaagtggtg tctgtcggcc
     2401 cgcttgccag gacggtgcct ttgaccatga ccgtataatc gcaaaatgtc gtgactgagc
     2461 cgagattgtg cgttgagaca agcatcgttt tgccttccgc gcgtaactcc ctcaacaggc
     2521 tgatgatttt cgcttcggtt ttcacatcaa cgccggtaaa aggttcatcc agcaggatca
     2581 catcaccctg ctgtgcaatc gctctcgcca gaaagacgcg tttcttttgt ccaccggaaa
     2641 gctcgccgat ttgacgatgg cgaaaatcca ccatatcgac acgttccagc gcatcggtga
     2701 caatctgacg atccctcttt ttggctatac gcaacatacc catatgccca tagcggccca
     2761 tcatcaccac atcttccacc agtacaggaa atgaccagtc aacctcttct gactgaggga
     2821 cgtaggcaac caggtttttc tgtagcgcct gtcgcgtggg aatacccaga acagatatct
     2881 ttccgctggt cagacgcaca aatcccataa tcgctttaaa caacgtcgat tttccggaac
     2941 cgtttacccc aaccagagcg gcaatagatc cacttggtac tgtgaaggat gcatcgcgca
     3001 gtgctgtgtg cccgttacgc caggtgacag taacatcatt aacgacaatg cctgcagatt
     3061 gcatcattat ttctcccttt tcccggcttt aattccctgt accagcgtac tggtagtaac
     3121 cttaagaagg tcgatatacg taggtaccgg gccgttttct gtgctcaggg aatcgacata
     3181 gagcacaccg ccgtagtgtg cgccggtttc acgcgcaacc tgacgcgctg gtttatcgga
     3241 aatcgtactc tcgctaaaga ctgccgggat atgatttttc ttaactatat caacaacctt
     3301 acgtacctgc tgcggtgttc cttgttgatc ggcattaatc ggccacagat aaagctcttt
     3361 tagccccaaa tcgcgtgcga gataagaaaa agccccttca ctggtgacca tccatcgctg
     3421 attctcaggg agttccgtaa tctgcttacg caggggggca agggtttggg taatcttggc
     3481 tttataagta tcggcattac gttggtaggt ttgtgcattt gccggatcgt attttatcaa
     3541 cgcatcacga atattatcga cgtaaatcag agcattatct ggcgacatcc atgcatgggg
     3601 attaggtttg ccctcatagg gtccttcggt gatccctact ggcgtcacac ccgaagagac
     3661 aattacttct ggaaccccat tgagatgctg gtaaaagcgt tggaaccaca attccagatt
     3721 cataccattg gcgagaatca gttgtgcccc ctgcgcacgt ttaatatcgc caggggtagg
     3781 ctgatactca tgaatttctg caccaggctt ggttatggat gagacttctg cagcatctcc
     3841 agccacgttt ttggccatat ccgcgatgat ggtaaatgtt gtaatgacct tgaatttttc
     3901 agttgcactt gcctgaaatg cgatcgagca ggtgagggcg aggcacccca agagcatggt
     3961 tacttttttt attgagtgca taatttagta cctatagtga gcaaaattag aacaaacata
     4021 gcaacggcta tgttttatag cataacccat acaaaatgaa aatgattatc attaacagct
     4081 ttgaggccta aaacagggat taggttgttt cctgctggcg acatgatatt gaggaaaaaa
     4141 gtagagcagc tattaggctt tgaaatgact ttatgaggaa aattgttgat ggcaaaaatg
     4201 gggcaaaacg ctgtagaagg ggcaaaaaag ggg
//
LOCUS       DAEEKQ010000257         4192 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.261, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000257 DAEEKQ010000000
VERSION     DAEEKQ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.261"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..215)
                     /gene="tuf"
                     /locus_tag="KLK57_004039"
     CDS             complement(<1..215)
                     /gene="tuf"
                     /locus_tag="KLK57_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE0894268.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVEYDT"
     gene            complement(330..405)
                     /locus_tag="KLK57_004040"
     tRNA            complement(330..405)
                     /locus_tag="KLK57_004040"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(370..372),aa:Thr,seq:ggt)
     gene            complement(412..486)
                     /locus_tag="KLK57_004041"
     tRNA            complement(412..486)
                     /locus_tag="KLK57_004041"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(451..453),aa:Gly,seq:tcc)
     gene            complement(603..687)
                     /locus_tag="KLK57_004042"
     tRNA            complement(603..687)
                     /locus_tag="KLK57_004042"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(651..653),aa:Tyr,seq:gta)
     gene            complement(696..771)
                     /locus_tag="KLK57_004043"
     tRNA            complement(696..771)
                     /locus_tag="KLK57_004043"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(736..738),aa:Thr,seq:tgt)
     gene            1133..2083
                     /gene="coaA"
                     /locus_tag="KLK57_004044"
                     /gene_synonym="panK"
     CDS             1133..2083
                     /gene="coaA"
                     /locus_tag="KLK57_004044"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HBE0894269.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2112..3077)
                     /gene="birA"
                     /locus_tag="KLK57_004045"
     CDS             complement(2112..3077)
                     /gene="birA"
                     /locus_tag="KLK57_004045"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HBE0894270.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3074..4102)
                     /gene="murB"
                     /locus_tag="KLK57_004046"
     CDS             complement(3074..4102)
                     /gene="murB"
                     /locus_tag="KLK57_004046"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HBE0894271.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1080 a         1042 c          936 g         1134 t
ORIGIN      
        1 gtgtcgtatt caacgtgaga agtgttgatg gtgataccac gagctttttc ttccggcgcg
       61 ttatcgatct ggtcgaatgc gcgagcagca ccgccgtagg ttttagccag tacggtagtg
      121 attgcagcgg tcagcgttgt tttaccatgg tcaacgtggc cgatagtacc gacgttaacg
      181 tgcggttttg tacgttcaaa cttttcttta gacatcgatt gtccctctaa gacacggata
      241 aatcggtgat atcaccacat caaccaggca acatgcccga cttgttgaat gcaataaaca
      301 gaaggaaaaa acagggagga gaaaagaagt ggtgctgata ggcagattcg aactgccgac
      361 ctcaccctta ccaagggtgc gctctaccaa ctgagctata tcagcacatc ttggagcggg
      421 cagcgggaat cgaacccgca tcatcagctt ggaaggctga ggtaatagcc attatacgat
      481 gcccgcatcc tggaactcgg ctacctgatt ttcattctgc actgaatatc gagagaagct
      541 ctctttattc gagccggtaa gcgaacttat cgtctcgggc tacgccatcg cgtggccgaa
      601 attggtggtg ggggaaggat tcgaaccttc gaagtctgtg acggcagatt tacagtctgc
      661 tccctttggc cgctcgggaa ccccaccgga cttgatggtg ccgactaccg gaatcgaact
      721 ggtgacctac tgattacaag tcagttgctc tacctactga gctaagtcgg catcaagtag
      781 cgcgcattct atggagacat gcgagttcat gcaactaaaa aattgcataa tttgttttat
      841 tggtcacatt ttatgcgaca cgatgaagaa acagccgaac ggagggcaga ttagcacact
      901 ttttcaacat cattgtgctc aacaatgcgc tcctgctaaa ccataattct ttttatcaga
      961 tggaatatct gtcacattgc ttttcaacga tagcttcctg gcagagattt tttcttatta
     1021 ttcctcccca tctggtgtta ccctcctgcc cattaaccca ttcaacagaa ctgtgacgcg
     1081 ccatggcaaa tatcgctttg ccgatagagc tatgaccgcc agaaacatgc ttatgagtat
     1141 aaaagagcaa acgttaatga cgccttacct acagtttgac cgcaaccagt gggcagctct
     1201 gcgtgattcc gtacctatga cgttatcgga agatgagatc gcccgtctca aaggtattaa
     1261 tgaagatctc tcgttagaag aagttgccga gatctattta cctttgtcac gtttgctgaa
     1321 cttctatata agctcgaatc tgcgccgtca ggcagttctg gaacagtttc ttggtaccaa
     1381 cgggcaacgc attccttaca ttatcagtat tgctggcagt gtcgcggtgg ggaaaagtac
     1441 aaccgcccgt gtattgcagg cgctattaag ccgttggccg gaacatcgtc gtgttgaact
     1501 gatcactaca gatggcttcc ttcaccctaa tcaggttctg aaagaacgtg gtctgatgaa
     1561 gaagaaaggc ttcccggaat cgtatgatat gcatcgcctg gtgaagtttg tttccgatct
     1621 caagtccggc gtgccaaacg ttacagcccc tgtttactcg catcttattt atgatgtgat
     1681 cccggatgga gataaaactg ttgttcagcc tgatatttta attcttgaag ggttaaatgt
     1741 cttacagagc gggatggatt atccacacga tccacatcat gtatttgttt ctgattttgt
     1801 cgatttttcg atatatgttg atgcaccgga agacttactt cagacgtggt atatcaaccg
     1861 ttttctgaaa ttccgcgaag gggcttttac cgacccggat tcctattttc ataactacgc
     1921 gaaattaact aaagaagaag cgattaagac tgccatgaca ttgtggaaag agatcaactg
     1981 gctgaactta aagcaaaata ttctacctac tcgtgagcgc gccagtttaa tcctgacgaa
     2041 aagtgctaat catgcggtag aagaggtcag actacgcaaa taatttgcag gggagcgaat
     2101 actccccttt cttatttttc tgcactacgc agggatattt caccgcccat ccagggtttt
     2161 attattccat cctgctcaag taataaagcc ccctgtttgt ctattccgcg tgaaatgcca
     2221 aatatttctt tatcaccaat gataagtttc actgggcgat taataaaatt atccagcttt
     2281 tcccagcgcg acagataagg tgccaatcct tcttgttcga agagttccaa cgcagcacgt
     2341 aattcacgta ttagcatggc cgccaacgta ttacgatcga gattgatccc cgcttcctgc
     2401 agcgtgatcc acccctgatt aacgacactc tcttcaacac ggcgcattgc catgttgatc
     2461 ccggctccaa tgactatttg cgccgcatcg ccagttttgc cagtcagctc cacaagaatg
     2521 cctgccagct tgcgatcctg cagatagagg tcattaggcc atttgacacg aactttatct
     2581 gctcccagct tgcgtaatac ttccgccatc acgataccga taaccagact taaaccaatc
     2641 gccgccgccg ggccttgttc cagacgccag aacatcgaca aatataagtt tgcgccaaaa
     2701 ggcgaaaacc atttccgccc ccggcgacca cggccagcct gctggtattc tgcaacacag
     2761 gcatcgcccg atttaagctc tccgatacga tcaagaaggt actgattcgt ggagtcaata
     2821 actggcagca cggctacact accgccatcc agctgaccca atatctgttc agcattaagt
     2881 aactggatgg gctcaggcag gctgtatcct ttacccggaa cggtaaagac atcaacgccc
     2941 cagtcacgca gtgtctgaat gtgtttatta atagccgccc ggctcattcc cagcgtttca
     3001 cccaactgct cgccagagtg aaattcaccg ttcgctaaca gggcaatcaa tttcagtggc
     3061 acggtgttat ccttcatgaa attgtctcca ctgcgctcac ttcacctgat gcaccaataa
     3121 agcggacttc aggctcaagc cagacattaa atttttcacc cactttttgt cttacatggt
     3181 gtgccagttg caccacatct tcgctttttg cattgtcttc attaatgaga actaacgcct
     3241 gttgacggtg caccgcagcc ccacccattt gcatcccttt tagctggcac tgatcaataa
     3301 gccaacctgc tgccagtttt actgaaccac ccgcctgggg gtaatttggt gctgttggaa
     3361 attgcgccag taatgcttta gccgtttcgg cagatacaac agggtttttg aagaaactac
     3421 cggcattgcc attcactttt ggatcaggga gtttggtggt gcgcatatga cacaccgcat
     3481 taaatacttg ctgtggcgtt actgttgtag gatccagacg agttaagtca ccatacgtta
     3541 gtacaggttg ccactctttt ggcagacgca gacctacggc tacaatggcg aagcgatcct
     3601 ggtattcatg tttaaaaata ctgtcgcgat agccaaaacg gcactctttg gcagttaagc
     3661 gcacttgctt gcctgtcgcc agttcaacac aatcaacata agcgcaaact cgctgtaatt
     3721 ctacgccata agcaccaata ttctggatag gtgatgagcc gacacaacca ggaattaatg
     3781 ccagattttc cagaccaggc ataccttcct gcaaagtgta ttttaccagg cgatgccagt
     3841 tttctccggc tcctacatgt aaataccacg catcaggttc atcatgaatt tcgatacctt
     3901 tgatccggtt gatgatcacc gtgccacgat agtcttccag aaaaagtaca ttacttcctt
     3961 cacccagaat aagaacgggt tgtccttctg cggttgcatg ctgccaggca ttgagtaatt
     4021 gttgttcgtc ttcggcacat acaatgtgct gagcattatg atcaatgcca aatgtgttcc
     4081 agggttttaa ggagtggttc atagctgctt tcctgatgca aaaacaaggc tagtttaccg
     4141 tatctgtggg gggatggctt gtagatatga cgacaggaag agtttgtaga aa
//
LOCUS       DAEEKQ010000258         4131 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.262, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000258 DAEEKQ010000000
VERSION     DAEEKQ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4131)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4131)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4131
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.262"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            115..528
                     /locus_tag="KLK57_004047"
     CDS             115..528
                     /locus_tag="KLK57_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HBE0894272.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(573..890)
                     /gene="hspQ"
                     /locus_tag="KLK57_004048"
     CDS             complement(573..890)
                     /gene="hspQ"
                     /locus_tag="KLK57_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HBE0894273.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="KLK57_004049"
                     /pseudo
     CDS             complement(948..2138)
                     /gene="rlmI"
                     /locus_tag="KLK57_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
     gene            2233..2511
                     /gene="yccX"
                     /locus_tag="KLK57_004050"
     CDS             2233..2511
                     /gene="yccX"
                     /locus_tag="KLK57_004050"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HBE0894274.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="KLK57_004051"
     CDS             complement(2508..2837)
                     /gene="tusE"
                     /locus_tag="KLK57_004051"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HBE0894275.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="KLK57_004052"
     CDS             complement(2928..3587)
                     /gene="yccA"
                     /locus_tag="KLK57_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HBE0894276.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIVHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(3794..3881)
                     /locus_tag="KLK57_004053"
     tRNA            complement(3794..3881)
                     /locus_tag="KLK57_004053"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3845..3847),aa:Ser,seq:tga)
BASE COUNT         1101 a         1047 c         1017 g          966 t
ORIGIN      
        1 atacttacta acatatagac atatttcccg cttttgctct cattcattcg tattagctgc
       61 atggttggca tgtcggcttt gtcgtacact ttgttaaaca gtctggagga aataatgaaa
      121 gaaaccgata ttgcaggcat tttaacgtcc actcacacca ttgcgctggt gggggcaagc
      181 gacaaacccg atcgtcccag ctatcgcgtg atgaaatatc tactcgacca ggggtatcac
      241 gtcattccgg tttcgccaaa agttgccggc aaaacgctgc tgggccagca ggggtatggc
      301 acgctggcag acgttccgga aaaggtcgat atggtcgatg ttttccgtaa ttcagaagcg
      361 gcgtggggcg tggcacagga agctattgcc attggcgcga aaaccctctg gatgcaactg
      421 ggggtgatta atgagcaagc ggcagtactg gcacgggatg ccgggttgaa tgtggtgatg
      481 gatcgctgcc cggctattga gatccctcgc ctgggcctgg ccaaataaaa aatccccgga
      541 aggcaaaaac cttccgggga ttcgttcagg gatcagttac gcagacgcgg ggcctggagt
      601 tgtttgcgga tggtctgcgc cagctcatcc atggatggct gttcaggatg ttcatcctgc
      661 aattcgctac ttagctgggc ttcagccagg taggtatgaa ccggtaggcc gttatcgtcc
      721 tccatcacta cgtgatacca cggagcggcg cgaagctcgt catttaccgc cagctcatca
      781 ggcgacggtt cagagagcga ataaaccggg tcgatatcca cgaccactcc gagataacct
      841 aacagggaat ggcggacctg ctggccgata ccgaatttgc tggcaatcat agtcacctcc
      901 cgggaaatct ttacctttga tataagggta aaattccaca tttcaagtta catgacgcga
      961 caggcaaacc ctttcagata tagcccttcc ggataggtag cgatcaccgg atgatcggct
     1021 gcctgacgga actgctctat aaattgtaca tcacggccgg catcaattgc ggcatccgcg
     1081 atgattttct gaaataaatc gctggtcatc agaccggaac aggagaaagt caggagaata
     1141 ccgccttcat tcagcaactg aatcgccagc atgttgatat ctttatagcc acgacacgcg
     1201 cccatcaact ggcttttatt ctcaacaaac ttcggcgggt ccatcacgat aacgtcaaat
     1261 ttttcaccgc gatcgcgata agtacgcagc aatttaaaga catcatcacg gacaaactca
     1321 gccttgctca gatccagttt gttcagctca acgttctgcc gtgcaatatc cagcgcttcc
     1381 taggaggtat caacgctgac aacctggctg caaccgccca tcagtgccga tacggcgaaa
     1441 ccaccggtat aggagaaaca gttcagcaca cgtttatttt caacgtagcg gcgggtagcc
     1501 aggcggctgt ctcgctggtc caggtagtag cccgttttgt gtccgtgctg aatatccacc
     1561 agcagcttca tgccgtgttc ttcaatcggc agcagggcag gcggcaactc gccggtgacg
     1621 aggccctggg tcagctccat cccttctttt ttacgtaccg caacatcgct gcgatcgtaa
     1681 atcgcacatt ccgggtacag cgtttgcagg gcactaatta atgccgcgcg ctgatattct
     1741 gccccagcac tcagcagttg cagcaccaga aaattaccga aacgatcgat agtaataccc
     1801 ggcaggccat cagattctcc ggcgattaaa cgatagctgt cgaggccatc tttttgcgcc
     1861 agtcagtcac gccatttttg tgcttgttgc aaacggcggg aaaaaaaagc aatgtcgata
     1921 gactcagacg ggtcaaacgt ccagacgcgc gcccggattt gcgaagctgg cgaataagcg
     1981 ccgcgtgcta accattttcc ctgatgatca acaatatcga tggtttcacc gaggctggct
     2041 ttaccttcca tgcgggcaac ggccccggaa aagacccacg gatggcgacg aagtaatgat
     2101 ttttcgcgcc ctttggctaa cactaaacgt acactcataa tttacttttc tgtcgatgca
     2161 aagaaatggg cgtcattgtc cggagttttc catggatttg caacgcgtcc attaaggata
     2221 aggagagcag caatgtcgaa agtctgcata attgcctggg tttacgggcg agttcagggc
     2281 gtaggatttc gctacaccac acagtacgaa gcgaaaagac tgggtttaac cgggtacgcc
     2341 aaaaatcttg acgatggcag cgtggaagtg gttgcctgcg gtgaagaagg gcaggtggaa
     2401 aaattaatgc agtggttaaa gagtggcggc ccgcgttctg cgcgtgtaga acgggtgctc
     2461 agcgagccgc atcatccctc gggggaatta accgattttc gtatccgtta aatacatttt
     2521 accggttttg gcaggccagc aattttggtg gcttgcttag ccggaccttt cggaaacagt
     2581 cggtataaat agcggctgtt acctttttct tcgccaaatt tattcgccat cgcttttacc
     2641 agcatacgaa tcgccggaga agtattgaat tccagataga aatcacgcac aaaacgcacc
     2701 acttcccagt gttccggtga cagcgaaatc ccttcgttct ctgcaatcac cactgccagc
     2761 ggctcactcc actggctgct ttctttgaga tagccttcgg tatccgtttc tatctcttta
     2821 ccttcgaaga tcagcataat tattactacc ttaatcagac tggcggcagt gtaacaaaaa
     2881 acaaagcccc gcataaagcg aggctatgaa agtgttagcg ggtgagatta atcgcggctg
     2941 gcgaagccca gaatgctcag caggctgacg aagatgttgt acagcgaaac atacaggcta
     3001 accgtggcac gaatatagtt cgtttcaccg ccatgaacga tgttgctggt ttcaaacaaa
     3061 atagcgccag aggagatcag aatgaagacc gcgctgatcg ccagatgcag agcaggcagc
     3121 tgcaggaaga tattagcaac cataccaatc agcaccacca caatacccgc catcagcata
     3181 ccgccgagga acgacatatc tttgcgggtg gtcagcacat atgcagagca gcagaagaac
     3241 actaacgccg ttccgcccag tgccatagcg attacgtcac ccattccggc agacagatag
     3301 gtgttcagaa taggtccgag gatataaccc agaaaaccgg taaaggcgaa tgcggagata
     3361 atcccggtcg gcttattcgc cgttttatag gtcaggaaca tcaaaccata catacccacc
     3421 agcgtcagaa tcagacccgg agatggcagc atcagcaccg tactggcagt tgcggtaatc
     3481 gccgaaaagg ccagcgtcag gctcagcaga aaataggtat tacgcagcac cttatgggtg
     3541 ctaagcagtg atgtacggtc atgtgaagaa ctaacaatac gatccattga gtcactctct
     3601 atgacagatg taattaatta agcagcataa tgataatgcg taagggcacc cagaagtttt
     3661 acccatcttt acgcatttga tctggaacag gtttaacagc ggattatcag gtcattaagc
     3721 aaatataacg ccctgagaat ttcgacaggc aaaagaaaaa ggggttagca tttagctaac
     3781 cccttatctt atttggcgga agcgcagaga ttcgaactct ggaacccttt cgggtcgccg
     3841 gttttcaaga ccggtgcctt caaccgctcg gccacacttc cggaatgacg cgcactataa
     3901 acatcccgat gcggcgtgta aacccctaat ttgtttgttt gcctgaaaaa cagccaaaag
     3961 tgcattgata gcgtgaaata acagcagatt gatcatttca tcaccatgaa ttccttctct
     4021 tttactcgtt tagcaaccgg ctaaacatcc ccaccgcccg gcaaaaagaa aaataggtcc
     4081 atttttatcg ctaaaagata aatccacaca gtttgtattg ttttgtgcaa a
//
LOCUS       DAEEKQ010000259         4124 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.263, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000259 DAEEKQ010000000
VERSION     DAEEKQ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4124
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.263"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..83
                     /locus_tag="KLK57_004054"
     CDS             <1..83
                     /locus_tag="KLK57_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0894277.1"
                     /translation="FWHDLAALVIAGILASMIVNWLNKRK"
     gene            459..566
                     /locus_tag="KLK57_004055"
     CDS             459..566
                     /locus_tag="KLK57_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0894278.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="KLK57_004056"
     CDS             complement(653..2332)
                     /gene="bcsG"
                     /locus_tag="KLK57_004056"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HBE0894279.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFATFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGASVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNISKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="KLK57_004057"
     CDS             complement(2329..2520)
                     /gene="bcsF"
                     /locus_tag="KLK57_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HBE0894280.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHFLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="KLK57_004058"
     CDS             complement(2517..4088)
                     /gene="bcsE"
                     /locus_tag="KLK57_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HBE0894281.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
BASE COUNT         1050 a         1030 c         1073 g          971 t
ORIGIN      
        1 ccttctggca tgatttagcg gctctggtca ttgctggcat tcttgccagt atgatcgtga
       61 actggctgaa caagcggaag taacgtgtca tgcgggcgtc aggctgccgt aatggcaatt
      121 tgcgcccgga ccaggccgca ggggtgaaac tctgcggcct ttttcgtaag aaaaatgcaa
      181 cgattgcacg cggataatat tctgcggaat gcgcagcaca gagtgaagac gtgacggatt
      241 ccggaacaga aaaacagtcg tagaatcagc ggcaagccgg aaaggttccg gtgaggcgca
      301 atgttgcggg ggctttatcc ctggtggcat tggttgctgg agagagaaaa cccccgcacg
      361 ttgcaggtat gcacctgaca acatcacggg ggctaatctt gaacccaaac aactcaagaa
      421 tagccgcgaa acgttggcat tacaacacag gcggctatat gacgctcgca gaactgggca
      481 tggccttctg gcatgattta gcggctccgg tcattgctgg cattcttgcc agtatgatcg
      541 tgaactggct gaataagcgg aagtaacgtg tcatgcgggc gtcaggctgc cgtaatggca
      601 atttgcgccc ggatcaggcc gcagaggtta aactctgcgg cctttttcgt gcttactgcg
      661 ggtaaggcac ccagtcgccg ccgttcaggc gaacgtacgg tttatcctgg tattgaataa
      721 ctactgcatt tgagttctcg gagaccggtg ctgtttgtgg caacccactg gtgagttttt
      781 tccagtcaac attgtcttcg gtgaaaatct tgccatcaag aacgcgaacc accagatcgg
      841 agatagctag gaagctgctc ggttggtcaa tgacaatcgg tgccccctga tgtggtgcct
      901 tcatgccgaa gaatttcacc ccaacgggga cgtctgtgat agacgggcta gggatatcac
      961 gtaggccaga tacctgcatt ctgtcgccct tcagcgcgcc gccgtgttcc ggcaccacga
     1021 ccaccatcac tttacgaccc gatttctcca gttcagtaaa gaaggcgtcc agttcatcaa
     1081 agaatttctg cgcccgcgct ttgtaatccg ctgttttgct gacccccgga taatggttgc
     1141 cgtcatgcag tggaagcgtg ttgtagaacg tggcactacg gctgttttta tctttttcgg
     1201 taacgtccag ccagcggtta agcacggcgg tatcgtcata aaccggcgaa ccatcaaagc
     1261 ccagcaaaat aaccggcaga tttgtttgat ccatcaattc agtctgcatg ccgccatttt
     1321 cgcgaacttc tttcaaaaaa ccgccgaact ggccgttatg tcccatcatc aggtgctggg
     1381 taaagcccag tttcgaaatg ttatcaaaca gatagcagtc gttatttgcc ggttgataca
     1441 gattagtgtg cgaagtctgc ccgcagctgg cgcgcagtaa acggatcgcc gccgggccac
     1501 tgtaggaggt ggcggagtta aagttcttga actcaatatc gaaatgcgac cacagtggat
     1561 gcgacatcaa cccggcggct tctatatccg accaggaaag cgaacagatg ttaatcacca
     1621 gtagttcaaa tggctgagca tcagcgggca gcgaagacgg gaaggtcgat ttacgtttcg
     1681 cctccgcgtt atagaaatta ttcagccagg cgttaaggtt cgccgttgtt ggcggtgcag
     1741 tttgtgcggg catatcaccc actaccgatg cgccccccgt cgccgcaacg gttgccgctg
     1801 cgttaccacc cgtcgttgtt acagtggtcg tcggttgtcc ggctggccac aaggagaaac
     1861 ttggtcccgc cagggtaagt acgttcagcc atagcagtat ggcaaccaca aaaacggtaa
     1921 tgcgaatcca ttgtgacagg aataaccagg ccactaataa aacaaaaatg gccccaatca
     1981 tctgccagtt aataaagcgt gtgacaaggt cgattaaata atcggtactg aaccccgcca
     2041 cctgcgaacc ctggctcatt atgctttccg ggccaggcaa ccaggtgtca tgccagaaca
     2101 aagcaaagcc gatcggcagg gcaatccagt ggcgcaagcg atgcaggctg tagcgcggaa
     2161 tgggcatcag cagaaacgtg gcaaacacca aattgaggag cggatggaag ttaagatatc
     2221 ccgcccacaa caggccgaac ttaaccagaa aatagaagtt ccagccggaa aggccgcgcc
     2281 agtattgcca gagggaagaa ggcatggcgg tattttgcgt aaattgagtc attttttggt
     2341 tgccctggct ttttccgtgc ggcgtaacgt cccggccggt ttaacataac gaggtttagc
     2401 aaagaataaa cgtaaggtgt cgcgaatgcg tcgcaaaaag tgccgcgcca gatagcccag
     2461 cgggaaaaat atcagtgcgc aaacgacaat aatttcaatg atatcgctga tggtcatcat
     2521 gatgagcgct ccacagcatc atctaacagt cgcatgggtt ctggtattcg tcgccagtgg
     2581 cgaccatcgt gctcggcatt gatgaccggt ttagaacttt gcgttaaagg cagcggcatg
     2641 ccccattgtt ctggggcaag caagcgcatc tgcaccagct cggcactgat ttgatcatct
     2701 tcaaaccaga ccatacggtt tgagaaaatg tcgccagtag gcaatgggaa aatatgattc
     2761 aacgcggtat ccagatcgtt aatccgacag aatgagagaa acagcaccag ccgattaccg
     2821 ccaatggtca tgatatcgcc ggtacggtta gggcgacaca gcgtcagggc ttgttcaacg
     2881 cggatacccg gtaccggacg tagggcaacc agaacgcctt taccgtgggc aggtaataga
     2941 gggttattca tcatgttgtt gacggcatta cagaacacat cccacttctg gaaaccacgc
     3001 agtttgagcg gctgggtcat tgacagcaag gtagtgatat cttccggcac atagcgacta
     3061 aacttctgcc cttgcacgct ttcgatcatc gtcagacaac gggagagtgg cgcattccac
     3121 ggaataacca tatttgcacc gcaggccaat aacaaacgtt catcggtggc gcgcaggcta
     3181 gcggtatttt cccgcacgag gattttcatc gcactaccgc gctggcgacg cagggtatga
     3241 atgctgcggg ccagtggctc gatttgcgca ttttgctgta aagaaaagac caccgtcgcc
     3301 gcctgagcgg tacgggcttc attgaacagg acttcgttat tgttgaacag ttgccagtgt
     3361 tccgatagcg gcggcgcacc ttccagtaca gcaacattac tcagaatgcg tttttcgtcg
     3421 ctgcgtggtt ggatctccgc ctcttcgctt tgaactaatg tccagatacc attttgttgc
     3481 tgaacgctaa gctgctgacg ggcgctgacc cctttttcgt tgcaccagaa ggcaatatcc
     3541 agcaaatgtt ggtcaccctg aaaacgcaaa ctggcaagac caaaaagtga acggtattcc
     3601 tcaagcaaca atgaaaattg tttatcgtta ttatttccgg gattaattac caaaagcgaa
     3661 caatggttta acctgctcca tttattcatt ttatccaacc atgagcgaag ccgctcggca
     3721 ggaatgtttt gccatgcgtt atttgcgcaa acaagaataa aaaggtaatt atggggatca
     3781 atagaacact gcaaatcacg ggccaaatag tatagacctt tttcatgatt tagcattgaa
     3841 aataatttta ttttttccgg cccttgagaa tcatctaatt gaaagatttt cgccggatcg
     3901 ctgtccatgc taatgaccgc gacgtgtgcg gtttcagcct gggatgcaat tgtttgattc
     3961 gccagactga tagcatcttc atggcgatcg acgttaaacc accagacgcc gcctgctggc
     4021 atatgtcgca gctcatccca taatgatgag ataccgatag agaatacagg gtccacaatg
     4081 tccctcatgt caccattttg tctgattatc agtttactag cgaa
//
LOCUS       DAEEKQ010000260         4089 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.265, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000260 DAEEKQ010000000
VERSION     DAEEKQ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4089
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.265"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..879
                     /gene="adeQ"
                     /locus_tag="KLK57_004059"
                     /gene_synonym="yicO"
     CDS             <1..879
                     /gene="adeQ"
                     /locus_tag="KLK57_004059"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HBE0894282.1"
                     /translation="IGLFIALMGLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFII
                     TVLSSRHFHAAVLVSIVVTSCCGLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLEL
                     AGIIFSFMLINLFDSSGTLIGVTDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTS
                     SVTAYIESTSGVAVGGRTGLTAVVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLM
                     TSSLARVNWDDFTESVPAFITTVMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLC
                     VVVVASLFALKIILVD"
     gene            932..1384
                     /locus_tag="KLK57_004060"
     CDS             932..1384
                     /locus_tag="KLK57_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HBE0894283.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            1595..2775
                     /gene="nepI"
                     /locus_tag="KLK57_004061"
                     /pseudo
     CDS             1595..2775
                     /gene="nepI"
                     /locus_tag="KLK57_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
     gene            complement(2816..3109)
                     /locus_tag="KLK57_004062"
     CDS             complement(2816..3109)
                     /locus_tag="KLK57_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894284.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3331..>4089
                     /gene="nlpA"
                     /locus_tag="KLK57_004063"
     CDS             3331..>4089
                     /gene="nlpA"
                     /locus_tag="KLK57_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HBE0894285.1"
                     /translation="MKLTTHHLRTGAALLLAGILLAGCDQSSSDAKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVALGNTFVFPMAGYSKKIKTVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQS"
BASE COUNT          901 a          907 c         1076 g         1205 t
ORIGIN      
        1 attggattat ttatcgcctt aatgggatta aaaaatactg gcgttattgt cgccaataaa
       61 gacacgctgg tgatgattgg cgatttaagt tctcacggcg tgttgttagg tattttaggg
      121 ttttttatta taaccgtgtt gtcatcacgt cattttcatg ccgcggtgct ggtttctatt
      181 gtggtgacgt cttgctgtgg attatttttc ggtgatgttc attttagcgg cgtctattcc
      241 attccgcctg atattagcgg cgtcattggt gaagtagatt tgagcggcgc gttaacactt
      301 gaactcgccg gtatcatttt ctcctttatg ctgatcaacc tatttgattc atcaggaaca
      361 ttaattggtg taactgataa agcgggctta atagatggta acggtaaatt ccccaatatg
      421 aataaggcgc tgtatgttga tagcgtcagt tcggtggcgg gtgcgtttat cggcacctcg
      481 tctgttactg cctatattga aagtacttct ggtgtggcag tcggtggccg cacggggctg
      541 actgcggttg tggttggcgt tatgttcctg ttggttatgt tcttctcccc gctggtggcg
      601 atggttcctc cttacgcaac cgccggagcg ttaatctttg ttggcgtgct gatgacttcg
      661 agcctggcgc gcgttaactg ggatgatttt accgaatcgg tgcctgcgtt tattaccacg
      721 gtgatgatgc cctttacttt ctcgatcacc gaagggattg cactcggctt tatgtcgtac
      781 tgcatcatga aagtgtgcac cgggcgctgg cgcgatctga acctgtgtgt ggtggtggtc
      841 gcatctctgt ttgcactgaa gattattctg gtggattagt aactggcgag ggtttccaga
      901 tatcatgagt tctgataacg caggagaact catgatctgg ataatgctcg ccacgctggc
      961 ggtagtgttt gtggttggtt ttcgggtgct gacatccggg gccagaaaag cgattcgccg
     1021 tctcagtgat cggctgaata tcgatgtcgt acccgtggag tcgatggtcg atcaaatggg
     1081 aaagtcagcc ggtgacgaat ttttacgtta tttgcatcgc ccggatgagt cgcacctgca
     1141 aaacgccgcg caggtgttgc tcatctggca aattgtcatt gtcgatggta gcgaacagaa
     1201 cctgctgcaa tggcatcgga ttttacaaaa agcccgcctt tccgcgccga ttaccgatgc
     1261 tcaggtcagg ctggcgctgg gttttctgcg cgaaaccgaa cctgaaatgc aggatattaa
     1321 tgcttttcag atgcgctata acgcgttctt tcagcctgcc gagggcgttc actggctgca
     1381 ttaaaaccgc ttcgtcttat cagcctacag accgcgcctg acctgtcggg tggataagac
     1441 gttcacgccg catccggtaa tctttgccct gttccgtttc gatcttaact ccagctcaag
     1501 caacaccgta aaagttgcaa aaacgttaaa tgcgtcacac atttcaatgt taaagtggtc
     1561 ggcttttccc ctgaaacatg ccacgggtaa caccatgagt gaatttattg ccgaaaaccg
     1621 cggcgcggat gccatcaccc gaccgaactg gtcagccgtt ttctcggtgg cgttttgtgt
     1681 cgcctgtctg attatcgttg agtttttgcc cgtcagtttg ttgacgccaa tggcccagga
     1741 tttaggcatt tcggaagggg ttgccgggca atcggtgacc gtgaccgcct ttgtggcaat
     1801 gtttgccagt ttgtttatta cccagacaat tcaggctact gaccgccgca acgttgttat
     1861 tttgtttgcc gttttgctga cgctctcctg cttgctggtt tcctttgcta actcattcag
     1921 tttgctttta atcggtcgtg cctgtctggg gctggcgctg ggcgggttct gggcgatgtc
     1981 ggcgtcgctg accatgcgtc tggtgccgcc gcgtacggtg ccgaaggcgc tgtcggtgat
     2041 cttcggcgcg gtttctattg cgctggtgat tgccgcgccg ttgggcagtt ttttaggcga
     2101 gcttatcggt tggcgcaatg tctttaatgc ggcggcggtg atgggcgtgc tgtgtatttt
     2161 ctggattatc aaatcattgc cttcactgcc aggcgaaccc tcgcatcaga aacaaaatac
     2221 tttccgctta ttacaacgtc cgggtgtgat ggcagggatg atcgccatct tcatgtcttt
     2281 cgccgggcag tttgctttct tcacatatat tcgcccggtg tatatgaacc tggcgggatt
     2341 cagcgtggat ggcttaacgc tggtgctgtt gagttttggc attgccagct ttatcggtac
     2401 gtcgctttcg tcattcattc ttaaacgttc ggtaaaactg gccttagcag gcgcgccgtt
     2461 aatactggct gtgagtgcgt tggtgctgac gttgtgggga agcgataaaa tcgttgctac
     2521 cggcgtggcg attatctggg ggctaacttt tgcattggtt cccgtcggct ggtcaacgtg
     2581 gatcacccgc tcactggccg atcaggcaga aaaagccggg tctattcagg tggcggttat
     2641 tcagcttgtg gcgcggcaat cggcggttat gcgctggata atattggtct gacttcgccg
     2701 ttgatgtttt ctggcacatt gatgttgctg actgcattgt tggttactgc aaaggtgaaa
     2761 atgaagaaat cctgatatca ggtaaagaag cgttggtcag tagttgttgc tgttcttaca
     2821 tatccgggca ttctacctta cccaacgctt cccaataatt gctctgatta ttacgttcca
     2881 tcgccacgat ggcctcgcgt atatgttgtt tattgttttc atctgacaac attaactttt
     2941 cccacgcttc atctgataat gacgcctgtg gcgtgcaaat tttacgtaaa tcttgtaata
     3001 ccgtggtttt ctcttttgcg gactgcagtg agctaaaagg cgactcggcg tatacgatgc
     3061 ctggcatagc gaaaaaagtg aaaataagca gtagtgtcgt tggtttcatg gattcaacct
     3121 tttacaaatg tgtatcgaat gaagcataag tgtagtttgc tttcatcgct ctcatcatgc
     3181 tgtacgaaac gcgcttgcgt ggtcagtaag aagtgccaga ctttatattc cacttttatt
     3241 cctttttatt cttatcgata gcgtttcgtt ttttaaaccg cagcgacctt accgctatag
     3301 tcaggtaatc attaataaaa ggataaaaaa atgaaactga caacacatca tctacggaca
     3361 ggggccgcat tattgctggc cggaattctg ctggcaggtt gcgaccagag tagcagcgat
     3421 gcaaaacaca ttaaagttgg cgttataaat ggcgcagaac aagatgtcgc ggaagtcgct
     3481 aaaaaggtgg caaaagagaa atatggcctc gatgttgaac tggtaggatt tagcggttcg
     3541 ttgctaccga acgatgcgac aaatcatggc gaacttgatg cgaatgtctt ccagcatcga
     3601 ccctttcttg aacaagataa tcaggcgcat ggttataaac tggtagcgct gggaaatacg
     3661 tttgtcttcc ctatggcggg ttattccaaa aaaatcaaaa ccgtggcgca aataaaagaa
     3721 ggcgcgacag tggcgatccc caacgacccg accaaccttg gtcgcgcact tttactgttg
     3781 caaaaagaga agctaattac tctgaaagag ggaaaagggt tattacctac cgcgctggat
     3841 ataaccgata atcctcgtca tttgcagatt atggaactcg agggggcgca gctcccgcgc
     3901 gtactggatg atcccaaagt tgatgtagcg attatcagca ccacttacat tcagcagaca
     3961 gggctttctc cggtgcacga cagcgtattt attgaagata agaattcgcc gtatgtgaat
     4021 attttggtgg cacgggaaga taataagaat gcagaaaacg tgaaggaatt tctgcaatct
     4081 tatcaatca
//
LOCUS       DAEEKQ010000261         4077 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.266, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000261 DAEEKQ010000000
VERSION     DAEEKQ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4077
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.266"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..668
                     /gene="erm"
                     /locus_tag="KLK57_004064"
     CDS             <1..668
                     /gene="erm"
                     /locus_tag="KLK57_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S ribosomal RNA methyltransferase Erm"
                     /protein_id="HBE0894286.1"
                     /translation="LNLKETDTVYEIGTGKGHLTTKLAKISKQVTSIELDSHLFNLSS
                     EKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVFESHASDIYLIV
                     EEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPRVNSVLIKLTRHTTDVPD
                     KYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQVLSIFNSYLLFN
                     GRK"
     gene            613..783
                     /locus_tag="KLK57_004065"
     CDS             613..783
                     /locus_tag="KLK57_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBE0894287.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSLD"
     gene            1298..1747
                     /locus_tag="KLK57_004066"
     CDS             1298..1747
                     /locus_tag="KLK57_004066"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894288.1"
                     /translation="MTTILSDYNKSILIELLNTNRQIIVVHGDDIDDYYTDSCYDIIF
                     IDYYDDDDFTYNGQEIWRGDSIYLVKSYQDEIQNRIEKGQELIFVNKENDISFSESIL
                     TYYHLFDYAGKTFEIDGQIIDEQTKVFAAPERRCACRTCWKVPARKT"
     gene            1708..2049
                     /locus_tag="KLK57_004067"
     CDS             1708..2049
                     /locus_tag="KLK57_004067"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894289.1"
                     /translation="MSHVLEGASQEDLNLYRAEVERDQAYGNWRDFFLNKKGSVTSVS
                     GDANLDQIADVSYLLDTFFAKVTRTALQNAASIAGLMITTEAMVAEAPKKDEPAMPPG
                     GGMGGMGGMDF"
     gene            2276..3808
                     /locus_tag="KLK57_004068"
     CDS             2276..3808
                     /locus_tag="KLK57_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019726358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBE0894290.1"
                     /translation="MPRLATSRRQAAGCAPLPSAHTGSRYARHAPERTLLYALVEAHY
                     PDFIARIEAEGRSLPGYVREAFDAYLRCGVLEHGFLRVVCEHCRAERLVAFSCKKRGF
                     CPSCGARRMAESARHLVEEVFGPRPVRQWVLSFPYPLRFLFASKPEAIGPVLGIVQRV
                     IAGWLADQAGIDRASAQCGAVTLIQRFGSALNLNIHFHMLWLDGVYVEATELPRRELR
                     LHRARAPTTAQLTQLAAAIAHRVCRHLTRKGWLEGEGESAFLADSAAGDDSMDGLRMS
                     SITYRIATGRDAGCKVVTLQTLPGDAGSLEGEAGKVGGFSLHAGVAAEAHESHKLEKL
                     CRYITRPAISEKRLSIALQGRVRYQLKTPWRNGTTHVEWDPVDFIAKLAALVPPPRAH
                     LTRFHGVFAPNAALRAQLTPSGRGRRHDAAVEPADASANDAPRSPEEKRRSMSWAQRL
                     KRVFSIDVTACVHCGGTVRIVASIEEPAAIRAILGHFVKQGAREEAHYRPAARAPPVQ
                     AA"
BASE COUNT         1018 a         1100 c         1086 g          873 t
ORIGIN      
        1 aattgaattt aaaagaaacc gataccgttt acgaaattgg aacaggtaaa gggcatttaa
       61 cgacgaaact ggctaaaata agtaaacagg taacgtctat tgaattagac agtcatctat
      121 tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt cactttaatt caccaagata
      181 ttctacagtt tcaattccct aacaaacaga ggtataaaat tgttgggaat attccttacc
      241 atttaagcac acaaattatt aaaaaagtgg tttttgaaag ccatgcgtct gacatctatc
      301 tgattgttga agaaggattc tacaagcgta ccttggatat tcaccgaaca ctagggttgc
      361 tcttgcacac tcaagtctcg attcagcaat tgcttaagct gccagcggaa tgctttcatc
      421 ctaaaccaag agtaaacagt gtcttaataa aacttacccg ccataccaca gatgttccag
      481 ataaatattg gaagctatat acgtactttg tttcaaaatg ggtcaatcga gaatatcgtc
      541 aactgtttac taaaaatcag tttcatcaag caatgaaaca cgccaaagta aacaatttaa
      601 gtaccgttac ttatgagcaa gtattgtcta tttttaatag ttatctatta tttaacggga
      661 ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt tacacgttac taaagggaat
      721 gtagataaat tattaggtat actactgaca gcttccaagg agctaaagag gtccctagac
      781 tagcaagaag tacacaagaa gccttaaaga ttatagaaaa gcttttccat gaaatgctta
      841 acattattta atgttaacat gtggctttgt ggtaactacc acgagtggta acttgtaaca
      901 tgaaaaaagt tacaagttac aagtggttta tttacgaaca taaaggctag gaaagcactt
      961 gataagtata tcaagtgctt tttttccgtt cgattttact atccaagata ccgcttgacg
     1021 tacctaatat tttagtgtgc taagggcttt gctttttctg gttatagttc gttcaaaaaa
     1081 gaccgcaagg gtaaatgcac gatcatagat cgcccttgca gctttttaat ctctccactt
     1141 ttttataacc aagcaaacca ccaagcacac tgaaaaaata attaggaact aaagcgaagt
     1201 gcgtatcttg gataaatctt cactcgattt taaaagatta agcacccagc caaagcgcct
     1261 aaaaaaataa tttttttaaa gaaaggaagt aatttaaatg actacaattt taagcgacta
     1321 caataaatct atcctaattg aattactaaa caccaatcgc caaattatcg tcgttcatgg
     1381 cgacgatatt gatgattatt acacagattc ttgctatgac attatcttta ttgattacta
     1441 tgatgatgat gatttcactt ataacggaca ggaaatatgg cgtggcgata gcatttatct
     1501 tgtaaaaagt tatcaagatg aaattcaaaa ccgtatagaa aaaggacaag agctgatatt
     1561 tgttaataaa gaaaatgata tatcattttc agaatctatt ttaacttatt accatctatt
     1621 tgattatgct ggtaaaacgt ttgaaataga cggtcaaatc attgatgaac aaacaaaagt
     1681 ttttgccgca cccgagcgcc gctgcgcatg tcgcacgtgc tggaaggtgc cagccaggaa
     1741 gacctgaacc tttaccgcgc ggaagtggag cgcgaccagg cctatggcaa ctggcgcgat
     1801 ttcttcctga acaagaaggg cagcgtcaca tccgtgagcg gtgatgccaa cctcgaccag
     1861 atcgcggacg tgtcgtacct gctggatacc ttctttgcca aggtcacccg caccgcgttg
     1921 cagaacgccg cctccatcgc gggcctgatg atcaccaccg aagccatggt ggccgaggcc
     1981 ccgaagaagg acgagccggc gatgccgccg ggcggcggca tgggtggcat gggcggcatg
     2041 gatttctgat gcggttggcc cggtcgtcag ggaaccggac cgcgccagcg cggtccgatc
     2101 ccggcaacga cccgacatca aggccccaag gacggggccg gagcccggca gcgatgccgg
     2161 gctttttgtt gtgcccgcgc cgcggcaatg tctgacgcga agatcagaac gcaccgatac
     2221 gaacgtgcga acacaggtgc aaccctgagc agccgtcccc gcaccggagc gctgcgtgcc
     2281 gcgcctcgcc acatcccggc ggcaagccgc gggatgcgcg ccactgccgt ccgcccacac
     2341 cggttcgcgg tacgcgcgcc acgcgcccga gcgcacgctg ctgtacgcgt tggtagaggc
     2401 gcactacccg gacttcattg cacggatcga agcggagggc cgctcgctgc ccgggtatgt
     2461 ccgcgaggcg ttcgatgcct acctgcgttg cggcgtgctc gagcacggct tcctgcgggt
     2521 ggtgtgcgag cactgccgtg cagagaggct ggtggccttc tcctgcaaga agcgcgggtt
     2581 ctgcccgagt tgcggcgcgc gacgcatggc cgagagtgcg cggcacctgg tcgaggaggt
     2641 gttcggcccg cggcctgtgc ggcaatgggt gctgagcttt ccgtacccct tgcgtttcct
     2701 gttcgccagc aagccagaag ccattggccc ggtgctgggc atcgtgcagc gcgtgatcgc
     2761 cggctggttg gccgatcaag ccggcatcga ccgcgccagc gcccagtgcg gcgcggtgac
     2821 gctgatccag cgtttcggca gcgcgctgaa cctgaacatc cacttccaca tgctgtggct
     2881 cgacggcgtg tacgtggaag ccaccgagct gccgcggcgc gaactgcgcc tgcaccgcgc
     2941 ccgtgcgccc accaccgcgc agttgaccca gctggcagct gccatcgcgc accgggtgtg
     3001 tcggcacctg acgcgcaaag gctggctcga aggggagggc gaatcggcct tcctggcaga
     3061 cagcgctgca ggcgacgaca gcatggatgg gctgcggatg agttcgatca cctaccgcat
     3121 cgccaccggc cgcgacgctg gctgcaaggt cgtcacgctg caaacgctgc ccggtgacgc
     3181 cggttcgctg gagggcgaag ccggcaaggt cggcggcttc tcactgcatg ccggcgtggc
     3241 ggccgaagca cacgaaagcc acaagctgga aaagctgtgc cgctacatca cgcgcccggc
     3301 gatcagcgag aagcggctgt cgatagcgct ccagggcagg gtgcgttacc agctcaagac
     3361 cccgtggcgc aatggcacca cgcatgtgga atgggatccg gtggatttca tcgccaagct
     3421 ggcggcgctg gtcccgccac cgcgcgccca cctgacccgc ttccacgggg tgtttgcccc
     3481 gaacgccgcc ctgcgcgcac agctgacgcc atcggggcgc ggcaggcggc atgacgccgc
     3541 tgtggagccg gcggacgcaa gcgcgaacga cgcgccgcgc agccccgagg agaagcgccg
     3601 ttcgatgagc tgggcgcaac gcctcaagcg ggtcttttcc atcgacgtca ccgcctgcgt
     3661 ccactgcggt ggcaccgtgc ggatcgtcgc cagcatcgag gaacctgccg ccatccgcgc
     3721 catccttggc cacttcgtga agcagggcgc gcgggaagaa gcgcactaca ggcccgcagc
     3781 gcgcgcaccg ccagtgcaag ccgcgtgacg atctgccggc tgcacagccg acggcgaaac
     3841 cggaatccga gccgatgcgg ccacgatccg cagggcggcg ctcggcccgc tgtcgggaat
     3901 cagcgaagca tggctgctga caacgccgct gcgtggcccc gcgatgccga aatcccactc
     3961 acagacgtcc gatccgtgcc caaaacgggg cttgcgcgac cgccgcctac ccagcagact
     4021 gcccgaaaag ggcgtttgaa cttcctatac gctgatagtg cattatctta aaatttt
//
LOCUS       DAEEKQ010000262         4048 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.267, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000262 DAEEKQ010000000
VERSION     DAEEKQ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.267"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            30..758
                     /gene="mpaA"
                     /locus_tag="KLK57_004069"
     CDS             30..758
                     /gene="mpaA"
                     /locus_tag="KLK57_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HBE0894291.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLAQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="KLK57_004070"
     CDS             complement(733..1698)
                     /gene="ycjG"
                     /locus_tag="KLK57_004070"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HBE0894292.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVSQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGVAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKIGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            1817..2323
                     /gene="tpx"
                     /locus_tag="KLK57_004071"
     CDS             1817..2323
                     /gene="tpx"
                     /locus_tag="KLK57_004071"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HBE0894293.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTDVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="KLK57_004072"
     CDS             complement(2367..3908)
                     /gene="tyrR"
                     /locus_tag="KLK57_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HBE0894294.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICVTQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVQRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
BASE COUNT          970 a         1143 c          971 g          964 t
ORIGIN      
        1 ctgtaatact tcctgaacca gagacgatca tgaccgtaac ccgcccacgc gccgaacgcg
       61 gcgcatttcc gcccggaaca gaacattacg gacgttcatt attgggcgcg ccgttaatct
      121 ggtttcctgc ccctgcagcc agccgcgaaa gtggtttgat tctggctggc actcacggtg
      181 atgaaaactc ttcggtcgtc acgctctcct gtgctctgcg gacgttgaca ccttctttgc
      241 gccgccatca tgtggtgctg tgtgtgaatc ctgacggctg ccagctgggg ttacgggcca
      301 atgctaatgg tgtggattta aacagaaact ttccggcggc gaactggaag gaaggtgaaa
      361 cggtttatcg ctggaacagc gccgctgaag aacgtgatgt cgtattactg acaggtgaca
      421 aacctggctc agaacctgaa acccaggcgc tgtgccagct tatacaccgc attcaacctg
      481 cctgggtggt ctcattccac gatccactgg cctgtattga agatcccaga cacagcgaat
      541 taggcgaatg gctggcccag gcgtttgaat tgcctctggt aaccagcgtc ggttatgaaa
      601 cgcctggctc tttcggcagt tggtgtgcag atctcaactt gcattgcatc accgcagagt
      661 ttccgcccat ctcctccgac gaagccagcg aaaaatatct ctttgcgatg gcaaacttgc
      721 tgcgctggca tcctaaagat gcaattcgcc cgtcgtgaac tgaagcgccg gttccacatc
      781 taccgccagc caggtcggtc cgtcaagatc ggcgaaactg acctgcggca ccagcggtaa
      841 agcggcgcta atggcacgag aggtacacaa catgcagccc agcatcagac tgaaaccttg
      901 tgcacgcgct tcagtcgcca gcgccagcgc ttccgtcaga ccaccgattt tatcgagctt
      961 aatgttaacc atctcatagc gccctttcag cgccttcaga ttgctacgtg tatgacaact
     1021 ctcatcagca caaatcggca acggatgaat aaaattctcc agcgccgcat catcctgcgc
     1081 aggaaggggt tgttcaagca tcgcaacgcc taaatccgcc aatagctggc aacgcgccgc
     1141 caccccttct gcacgccagg attcatttgc atcaacgatc agcgtcgcat cgggcacagc
     1201 tgtgcgaatt gccaccatcc gctcactaat aagatggtta tccagcttca ctttcagtaa
     1261 tttcgcgcct gcctgccaga gtgttgatgc actattggcc atctgatcag gcgtaccgat
     1321 gactaccgtc tgtgcagtga taactgtctc gggaagcgtt atgccgatca aatcagccag
     1381 cgattgctgc tgttttcgcg cggccagatc ccacaatgca caatccagcg cattacgtgc
     1441 tgcgccggca gggagaattt tttgcaactc ctcccgtgtc agccctttct ctaattgcga
     1501 cacgacgctc ataatttgcg ccattaccga ggcatcactt tctccataac gcggatacgg
     1561 cgtgcattcg ccggtgcctt taataccctc ttcttccagt tcaaccacca ccacgcgcgc
     1621 ttcactgcga cttccccggg caatcacaaa cggggtatgt aagggccagg cttcctcaaa
     1681 taccttaacg gttctcatct ttactccttg ttgtcgcggt ggtctgcgga ttaagcgcaa
     1741 atagcgtttg ctgtgttatt gacagttagc ataaactagg tgtgacgtta actatatgta
     1801 aacaggaaag ataatcatgt cacaaaccgt tcatttccag ggcaacccgg ttacagtcgc
     1861 caattccatc ccgcaggcgg gtagcaaagc gcagactttt actctcgtgg caaaagatct
     1921 gtctgacgtc accctcggtc agtttgcggg taaacgcaaa gtgctgaaca ttttcccgag
     1981 tattgatacc gatgtttgcg ccgcatcagt acgcaagttt aaccaactgg caactgagat
     2041 cgacaacacc gttgtgctgt gtatctctgc cgatctgccg ttcgcccagt ctcgtttctg
     2101 cggcgcagaa ggtctgaaca acgttatcac cctctccact ttccgtaacg ctgaatttct
     2161 gcaagcttac ggtgtggcaa ttgctgatgg cccactgaaa ggtctggcag cgcgtgccgt
     2221 tgtggttatt gacgaaaatg acaatgtgat tttcagccag ctggtggatg aaatcaccac
     2281 cgagccggat tacgaagcag ctctggctgt actgaaagca taagttaata tgcctgatgg
     2341 tgttgcacca tcaggcatat tcgcgcttac tcttcgttct tcttctgact cagaccatat
     2401 tcccgcaact tattggcaat cgcggtatgt gaaacgccga gacgttttgc cagtttgcgc
     2461 gtgctgggat aattgcgata aagctgggtt aataccgagc gttcaaaacg gctggtgatt
     2521 tcgtccagcg aaccttccat cgcatcttcg cccacggcta ccgttgcggc gtcataatcc
     2581 ggcaacaaaa tatcctgtgg acgcagctca taaccgtcca gttgtgtcag tgcgcgatag
     2641 atagcgttct ttaactgccg cacatttccc ggccacgcat aacgcgtaag tacagtattc
     2701 aggtcagcgg ccagtttcgg acgctgcacg ccctgctcgt cggcaaagcg ggcgacgaac
     2761 agctcagtta acggcatgat gtcctgcgga cagtcacgta gcggcggcag attgagcgtc
     2821 aacacgttca gacgataata gagatcttca cggaacatgc ctttttgcac cagttcgacc
     2881 agattcttct gcgtaacgca aatcacccgc acatcgacat gcacctcatg gtcttcgcca
     2941 acccgacgga aagtgccatc attaaggaaa cgcagtaatt tcgcctgcat ccgtggtgac
     3001 atttccccta tttcatccaa cagcaccgaa ccaccgttcg cctgctcaaa gaatcctttc
     3061 ttcccttccg gagcatgacc aaacagttca ctctcgaccg catcttccgg tatagacgca
     3121 cagttcagcg ccaggtaagg tttgcccgct ctggggcttg cctgatggca ggcgtaggca
     3181 aagagatctt tacctgtacc tgtgtcaccc gtaatcagca gcggcgcgct tagcatcgcc
     3241 agtttctgcg cctgttcgac aacatgcttc attttcgggc tgacggcgac aatttgactg
     3301 aaggcgctga cgtcctgggc ggcgacattt tgcaactggc ggcccatacg aatcgttgat
     3361 cgcaacatca ccaccgcacc ggtcaggacg tgttgatcat tttcatcctg aagataaaca
     3421 ggcgtaatct ccatcaggaa attctgccca ttaataacga catgctcgtt atgcgaatct
     3481 tgcggttcgc tttccagcca acgtaaaaaa ttaaagccgt taatcaattg tgcggcggta
     3541 tggttgcgca ggcgatccaa tttttgccca aaaagctgac agctcgccgg gttcgccata
     3601 tccactttgc ttttcatatc gacagagagc acaggttcag gcaacgcctc cagtaacgcg
     3661 ctcaacgcca gatgctcacg ttcggaaggc atccacggga cagtacgcac atcggtaaca
     3721 cccgcaatac ggcgtatttc ggccatcaga ctgctgaaac tctcaaactc cagttcagca
     3781 aaattgaggt agattcgccc aatgggatca atctcaatac cgcgtaaatc aatgcctctt
     3841 agcacgagta gatcgagtaa ttcgcgggtc agaccgagtc ggtcttcaca aaagacttcc
     3901 agacgcatgg gaaccttcac ctgaaaaaag aacaattaat atgatgatat gacactattt
     3961 ggatagcagg aaggtttctg tcaacaatca ttgacacaaa gacggaaaaa cgggcacttc
     4021 ggcgtaaaga tatccggtca gcacccga
//
LOCUS       DAEEKQ010000263         3951 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.268, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000263 DAEEKQ010000000
VERSION     DAEEKQ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.268"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            234..1898
                     /gene="tssM"
                     /locus_tag="KLK57_004073"
                     /pseudo
     CDS             234..1898
                     /gene="tssM"
                     /locus_tag="KLK57_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354198.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
     gene            1909..3261
                     /locus_tag="KLK57_004074"
     CDS             1909..3261
                     /locus_tag="KLK57_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001087757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HBE0894295.1"
                     /translation="MNSNVLTQTIVTGSDPRGLPEFSAIREEINKASHPSQPELNWKL
                     VESLALAIFKANGVDLHTATYYTLARTRTQGLAGFCEGAELLAAMVSHDWDKFWPQGG
                     PARTEMLDWFNSRTGNILRQQISFAESDLPLIYRTERALQLICDKLQQVELKRVPRVE
                     NLLYFMQHTRKRLEPQPKSNAENAAQTTVRTLIYAPETLASSTPEAVVPPLPGLPEMK
                     VEVRSLTENPPQASVIKQGSTVRGFIAGIACSVAVASALWWWQVYPVQQQLLQVNDTA
                     QGAATVWMASPELENYERRLQQLLDTSPVQPLETGMQMMRVADSRWPESLQQQQASTQ
                     WNEALKTRAQSSPQLRGWLQTRQDLHAFADLVMQREKEGLTLSYIKNVIWQAERGLGQ
                     ETPIESLLTQYHDARAQKQNTDALEKQINERLEGVLSRWLLLKNNVMPEAATGTTAEK
                     "
     gene            3285..3767
                     /locus_tag="KLK57_004075"
     CDS             3285..3767
                     /locus_tag="KLK57_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HBE0894296.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
BASE COUNT         1060 a         1019 c         1062 g          810 t
ORIGIN      
        1 tggaacagac ctatctgcaa ctgctggagc aacgttacct gccctcgctg tttaacgggc
       61 tggtcaaagc gatgaacgcc gcgccgccgg agagtgaaga aaaactcgcg gtgctgcgcg
      121 tgatgcgaat gctggaggac aaaagcggac gtaacaatca ggtggtgaag cagtatatgg
      181 caaaacgctg gagcgaaaag ttccacggtc agcgcgatat ccaggcacaa ctgatgtccc
      241 atcttgacta cgcgctggct catactgact ggcacgcaga gcgtcaggcg ggcgacggtg
      301 acgccatcag ccgctggacg ccatatgaca agcccgtggt atcagcacag aaagaactga
      361 gcaaactgcc tgtctaccag cgggtttacc agagcctgaa aacgcgggcg ctgggcgttc
      421 ttcctgccga cctcaatctg cgtgaccagg tagggccaac ctttgaccag gtgtttacat
      481 ctgccgatga caacaaactg gttgttccac agtttatcac ccgctacggc ctgcaaagct
      541 attttgtaaa acagcgcgat gaactggttg aactgacagc gatggattcc tgggtactta
      601 acctcacccg caatgtgaaa tacagtgacg ccgaccgtgc ggaaatccag caccagttga
      661 ccgagcagta tatcagcgac tacaccgcca cctggcgggc tgggatggac aatctgaata
      721 tccgcaattt tgagtccatc ggacaactga ccggggcgct ggagcaggtt atcagcggcg
      781 accagccttt gcagcgggcg ctgaccgtgc tgcgtgacag cacacagcca ggcgtctttt
      841 ctgaaaaact ctctgccaaa gaacgggagg aagccctggc agagccggat taccagttac
      901 tcacccgcct cgggcatgaa ttcgccccgg aaaacagtac cctggcagta cagaaagaca
      961 aagaaagcac gatgcaggcc gtgtatcagc aactcaccga gttgcaccgc tacctgctgg
     1021 caatccagaa cgcgcctgta ccagggaaat cggcgctgaa agccgtgcag ttacggcttg
     1081 atcagaacag cagcgatccg atattcgcca cccgccagat ggcaaaaacg ctgcctgctc
     1141 cgctcaaccg ctgggttggc agactgactg actaggcctg gcatgtggtg atggtggagg
     1201 ctgttcatta tatggaagtg gactggcgcg acagcgtggt gaaaccgttt aacgagcaac
     1261 tggcaaataa ctatccgttt aatccgcgtt ctgcacagga tgcctcactg gatgccttcg
     1321 aacgcttctt taaaccggat ggcatactgg ataccttcta ccagcagaac ctgaagctgt
     1381 ttatcgataa tgacctgagt ctggaggatg gcgataacaa cgtcattatt cgcgaagata
     1441 ttattgcgca actggaaact gcgcagaaaa tccgtgacat cttcttcagc aaacagaacg
     1501 gtctgggaac atcctttgcc gtggaaacgg tatcgctttc aggcaataaa cgccgcagtg
     1561 tactgaacct tgacagtcag ttagtcgatt acagccaggg ccgtaactat accgcccatc
     1621 tggtctggcc taacaacatg cgcgaaggca acgaaagtaa gctgacgctc atcggcacca
     1681 gcggcaacgc gccgcgcagt atcagcttca gcgggccgtg ggcgcagttc cgcctgttct
     1741 gggccggaca actgaccgga gtacaggatg gcaactttac cgtgcgcttt agcgtggacg
     1801 gtggcgcgat gacctaccgt gtgcataccg acacggaaga taacccgttc agcggtgggt
     1861 tgttcagcca gtttggtctg tcagacacac tgtactgagg aggcagggat gaatagtaac
     1921 gtactgacac aaactatcgt taccggcagt gacccgcgcg ggctgccgga attcagcgcc
     1981 atccgcgagg aaataaacaa agccagccac ccgtcacagc ctgagctgaa ctggaaactg
     2041 gtggagtcgc tggcgctggc gatttttaaa gccaacggtg tggatttaca caccgccacc
     2101 tactatacgc ttgcccggac acggacacag ggactggcgg gattctgcga aggtgcggaa
     2161 ctgctggcgg caatggtaag ccacgactgg gataagttct ggccgcaggg cggcccggcg
     2221 cgtactgaaa tgctggactg gtttaactcc cgaaccggca atattctgcg tcagcaaatc
     2281 tcctttgcgg aatccgacct accactgatc taccgcacag agcgggcatt gcagcttatc
     2341 tgcgataagc tccagcaggt ggaactgaag cgcgttccgc gcgtggagaa tctgctctat
     2401 tttatgcagc acacgcgtaa acggcttgaa ccccagccga agagtaacgc tgagaacgcc
     2461 gcacagacca cggtcagaac gctgatttat gccccggaaa cactggcatc ttccacacca
     2521 gaagcggtag tgcctcccct gcccggcctg cctgagatga aagtggaagt gcgcagtctg
     2581 acagagaatc ccccacaggc cagtgtgata aagcaaggca gtacggtaag agggtttatc
     2641 gcagggatcg cctgttcagt ggctgtcgcc tcagcattgt ggtggtggca ggtctatccg
     2701 gtgcagcagc aactgttaca ggttaacgac accgctcagg gcgcagcaac ggtgtggatg
     2761 gcctcacctg aactcgaaaa ctatgagcgc aggctgcaac aacttcttga tacctccccg
     2821 gtacagccgc tggaaaccgg gatgcagatg atgcgtgttg ccgacagtcg ctggccggaa
     2881 agcctgcaac agcaacaggc ctcgacacaa tggaatgagg cactcaaaac ccgcgcacag
     2941 agtagcccgc agttgcgtgg ctggttgcag acccgccagg acttacatgc ttttgcagat
     3001 ctagtgatgc agcgcgagaa agagggacta accctttcct atatcaaaaa tgtcatctgg
     3061 caggcggagc ggggactggg gcaggaaacc cccattgagt ctctgttgac gcagtaccat
     3121 gatgcccgtg cgcagaagca gaatacagat gcgctggaaa aacaaattaa tgagcgactc
     3181 gaaggcgtgt taagccgctg gctgctgctg aagaataacg tcatgccaga ggcggcaacc
     3241 ggtaccacgg ccgaaaaata attatttaat taaaggagat tatcatggca aatataagtt
     3301 atttatcttt gagtggagag acacaagggc taatttcagc aggttgctca acgcttgatt
     3361 ctgtcggaaa caaagcgcag ccagaacata aagatcagat catggtttat gcgttaatgc
     3421 attccatctc aagaagtcaa aatgtcaatc atcatgaatt aattatcacc aaaccggttg
     3481 ataaatcctc accattgctg gcaaaagcac ttagtgacaa tgaaaaaatg gcaatatgtg
     3541 aatttatact ctaccgaacc agtaaagcgg gtatttacca gccttattat aaaataaatc
     3601 tgtcaaaagc ccgaatatca agtattgatt ttgtcacacc gcatgctgtc ctggaaaaag
     3661 aactggaacc acaggagaga attgccttta tttatgaaga tatttcctgg gagcatacgt
     3721 tagccggtac gaatgcaatg agtaaatggc aggatcgtgt acagtaactt actgtaataa
     3781 cataataaat tactattgcg gagagataaa atggctggtt taaggaataa caacaacaca
     3841 cagaatgcac agtgggctga ttatgtgggt gacattcttc ggggggctca gccgataaac
     3901 cagctagtac ctcagcaccc ttatcttaac gacgtcccac tcattgatga g
//
LOCUS       DAEEKQ010000264         3950 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.269, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000264 DAEEKQ010000000
VERSION     DAEEKQ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.269"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..952)
                     /gene="ydcO"
                     /locus_tag="KLK57_004076"
     CDS             complement(<1..952)
                     /gene="ydcO"
                     /locus_tag="KLK57_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HBE0894297.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLTLTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMRIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FSFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLF"
     gene            1005..1541
                     /gene="sutR"
                     /locus_tag="KLK57_004077"
     CDS             1005..1541
                     /gene="sutR"
                     /locus_tag="KLK57_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HBE0894298.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSAMPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            1614..3575
                     /gene="rlhA"
                     /locus_tag="KLK57_004078"
     CDS             1614..3575
                     /gene="rlhA"
                     /locus_tag="KLK57_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HBE0894299.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLTRAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLNV
                     AVTEPLANGDGLNVMIKREVIGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHNWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVFPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(3667..3897)
                     /locus_tag="KLK57_004079"
     CDS             complement(3667..3897)
                     /locus_tag="KLK57_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HBE0894300.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
BASE COUNT         1023 a         1007 c         1002 g          918 t
ORIGIN      
        1 caaacagacc tgcgagcaaa tagaaaatgc ctgcaacggc agctgccagc caacgttgat
       61 ctttatccgg atgcgcttcc gggctttggc aaatagccgc ggtgattgcc gcaataccga
      121 cggaataaac gccgaaaggg gaaaaaacca gtgccagcaa tccagtaaat acaattaatg
      181 gcgaaacagg agccgaatat ccagctgctt tcattgctgc gatacccggt gcgttttgcg
      241 atgccatcgt caccagaaaa agggggagtg caacgctcag gctgtgagca aacgaaaaat
      301 caggggtaat ataagtgggg agaacgggtt taaagacaac atcagttgtg acaacgtcac
      361 cttgcgcgat aacgatcacg atcccaataa tcatcgcggc aattaccgca tagcgcggcg
      421 caacggcctt ggttgccagc cataccagca acatacttcc acacaacgta aattgaccgt
      481 ccagactggc aaacgcctgt aaaccaaagc gtaataaaat cccggcaagc attgccgccg
      541 caagcgagtg cggaataatg cgcatcagac gagcaaagag tcccgttatg ccgcagagga
      601 ctattaacgc gttggtgaca ataaaaacgc cgatggcttc gttaagtgtt agtccctgca
      661 atccggtgac caacaaagcc gcgccaggcg ttgaccatgc ggtgagaaca ggtacgcgat
      721 accataatgt cagagtcagc gtactgacgc ccattgccag ccccagcgcc gtcatccagc
      781 cagagatttg tgcagtggtg gctccggcga caatcgctgc ttgccagatt attgccgctg
      841 aactggcgta gccaattaat accgccagaa accccgccag tagcgtgggt ggaggaatag
      901 aaaacagacg catagtcacc tcgtgcgtta tagcgtacag gcaccgtagc atttgtccgt
      961 tataacgcac aagtgataaa cttccgtttt gccggaggag tcgcatggaa aatctcgctc
     1021 gctttttatc cactacactt aaacaactac gccagcagcg cggctggagt ctttcgcgac
     1081 tggcagaagc gacgggcgtt tctaaagcaa tgttagggca gattgagcgt aatgagtcca
     1141 gtccgacggt cgcgacatta tggaaaattg ccaccgggtt gaacgtacca ttttcaacat
     1201 ttatttctcc gccgcagtcc gctatgcctt cagtttatga tccacaacag caggcgatgg
     1261 tgatcacttc gctgtttcct tatgatccac aactctgctt tgaacacttc tcgattcaga
     1321 tggcatctgg tgcaataagc gaatcgacgc cccatgagaa gggggtaatc gaacatgtcg
     1381 tcgtcattga tggacaactt gatctgtgcg ttgatgggga atggcagacc cttaattgtg
     1441 gggaaggcgt tcgatttgct gcagacgtca cgcatatcta tcgtaatggt ggggagcaaa
     1501 ccgtacattt tcattccctc atccattacc cgcgcagtta agcgggaaaa ctatttcgca
     1561 acgtcgcgct tgtggctaaa atagccgcca tttttcagct actggataag aatgtgaccg
     1621 tatcttctca tcgacttgaa ctgttaagcc cggcacgcga tgccgccatt gcccgcgaag
     1681 ctattttgca cggtgccgat gctgtttata tcggcggccc tggttttggt gcccgtcata
     1741 atgccagtaa tagcctgaaa gatattgccg agctggtgcc gtttgcccat cgttatggtg
     1801 caaaaatttt cgtcacgctt aacaccattt tgcatgatga tgagctggaa cccgcgcaac
     1861 ggctgattac tgacctctac cagaccggtg tcgatgcgct gattgttcag gatatgggga
     1921 ttctggaact tgatattccg ccgattgaac tgcacgccag tacgcagtgc gacattcgta
     1981 cagttgaaaa agcgaagttc ctctctgatg ttggcttcac gcagattgtg ctggcgcgag
     2041 agctgaatct tgatcagatc cgcgcgattc accaggctac ggacgcgacc attgaattct
     2101 ttattcatgg ggcactgtgc gtggcctatt cgggtcagtg ctacatttct catgcgcaaa
     2161 cagggcgtag cgccaaccgt ggcgattgct cgcaggcgtg ccgtttgcca tacacattga
     2221 aagacgatca ggggcgggtg gtttcctatg aaaaacatct gctgtcgatg aaagataacg
     2281 atcagactgc caacctcggc gcgctgattg atgctggcgt acgctccttc aagattgaag
     2341 ggcgttacaa agatatgagc tacgtgaaga atatcaccgc ccattatcgc cagatgctgg
     2401 atgccattat tgaagaacgt ggcgatctga cgcgtgcttc atcaggtcgt actgaacatt
     2461 tctttgttcc atcgacggaa aagactttcc accgtggtag cacagattat tttgtgaatg
     2521 cccgtaaagg cgatattggc gcgttcgatt cgccgaaatt tatcggcctg ccggtgggtg
     2581 aagtattgaa agtggcgaaa gatcatctca atgttgccgt caccgagcca ctggcaaatg
     2641 gtgatggcct gaacgtgatg attaaacgtg aagtcatcgg ttttcgtgcc aatacggtcg
     2701 agaaaaccgg agaaaatcag taccgcgtct ggcccaatga aatgccagca gatttgcaca
     2761 aaattcgtcc acatcaccca ctaaaccgta atcttgatca taactggcag caggcactga
     2821 caaaaacctc cagcgaacgt cgggtggcgg tagacattga actgggcggc tggcaggaac
     2881 aactgattct gaccctcacc agtgaagagg gtgtcagcat cacgcatacg ctggacgggc
     2941 agttcgacga agccaataac gccgaaaaag caatgaacaa tctgaaggat ggtctggcaa
     3001 aactggggca aaccctctat tacgcccgcg atgtgcaaat taatttgccg ggggcgctgt
     3061 ttgtaccaaa cagtctgtta aaccagttcc gccgtgaagc tgctgacatg ctggatgctg
     3121 cgcgtcttgc cagttaccag cgcggcagcc gtaaaccggt tgctgatcct gcgccggttt
     3181 ttccgcaaac gcatctgagt ttcctcgcga acgtatacaa ccagaaagcg cgtgaatttt
     3241 atcatcgcta tggtgtgcag ctgattgacg cggcgtatga ggcacatgaa gagaagggcg
     3301 aagtcccggt gatgatcacc aagcattgtc tgcgctttgc ctttaatctg tgcccgaaac
     3361 aggcgaaagg caatatcaaa agctggaagg cgacgccaat gcaactggtt aacggcgatg
     3421 aagtattaac gctaaagttt gattgccgcc catgcgagat gcacgtcatt ggcaaaatca
     3481 aaaatcacat actgaaaatg ccgttaccgg gaagcgtagt ggcatccgta agtccggatg
     3541 agctgctgaa aacattgcct aagcgaaaag ggtaaaacgc cagttttctg gttactcaca
     3601 actcattgaa tctgcatgat attgcctgcc gggtaaggcg tcacgccgca tccggcatca
     3661 aacgactcaa cgttgtcccg cttctggttt gcgcgatttt tgccagtaaa aatgctcgcg
     3721 caaaccttcc gccgactctt ccgccacagc acgcaattca tcgctgtccg cttcatgacg
     3781 cagctgatga tccacattct ttacccacac aaattcatgt cctttgtgcc ctgccatgag
     3841 ttgtcctgag aacagcgcac acgttaataa gacaaccgat aacgccttcg taaacatcct
     3901 gccacctttt tcttaccttt tgccgctatg atgccgatcg tttcttgagg
//
LOCUS       DAEEKQ010000265         3908 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.270, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000265 DAEEKQ010000000
VERSION     DAEEKQ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.270"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            24..590
                     /locus_tag="KLK57_004080"
     CDS             24..590
                     /locus_tag="KLK57_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HBE0894301.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            594..1169
                     /locus_tag="KLK57_004081"
     CDS             594..1169
                     /locus_tag="KLK57_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HBE0894302.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1211..2263)
                     /locus_tag="KLK57_004082"
     CDS             complement(1211..2263)
                     /locus_tag="KLK57_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HBE0894303.1"
                     /translation="MPVLHNRISNDALKAKMLVESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDALFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2488..3408
                     /gene="lpxL"
                     /locus_tag="KLK57_004083"
     CDS             2488..3408
                     /gene="lpxL"
                     /locus_tag="KLK57_004083"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HBE0894304.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGIQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3580..3777
                     /locus_tag="KLK57_004084"
     CDS             3580..3777
                     /locus_tag="KLK57_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HBE0894305.1"
                     /translation="MSPCGNDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIIPS"
BASE COUNT          971 a          988 c          950 g          999 t
ORIGIN      
        1 gcaaattcgt attggatgca cttatgtcat tcacaaatac ccctgaacgc tatggtgtta
       61 tatctgcggc cttccattgg ctaagcgcaa ttattgtcta cggcatgttt gctttaggtc
      121 tgtggatggt cacgctcagt tattacgatg gctggtatca caaagcaccc gaactgcata
      181 aaagcatcgg tattttgtta atgatggggc tggttattcg cgttctgtgg cgtgtcatat
      241 ctccaccgcc gggtccgctg ccgagttatt cgcccatgac tcgtcttgcc gcaaaggctg
      301 gacatctcgc attgtaccta ttgctgttcg ccatcggcat cagcggctat ctgatctcaa
      361 ctgccgatgg taaaccgatc agcgtttttg gctggtttga cgtccccgcg acccttgccg
      421 acgctggcgc acaggcagac tttgccggtg ccctgcattt ttggctcgcg tggagcgtcg
      481 tagtactgtc cgttatgcac ggatttatgg ccctgaagca tcatttcatc gataaagacg
      541 acactctgaa gcgcatgctg ggaaagtcgt catctgacta tggagtataa aaaatgaaaa
      601 aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc tgccggttca gcggttgccg
      661 ccgattacaa aattgacaaa gaaggtcagc acgcctttgt taatttccgc atccagcacc
      721 ttggctatag ctggttatac ggcaccttta aagatttcga cggtactttt acctttgacg
      781 aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa caccaccagc gtcgatacta
      841 atcacgccga acgcgataaa catcttcgca gtgcagattt cctcaatacc gcaaaatatc
      901 cacaggcaac attcacctcc accagcgtga agaaagacgg tgacgaactg gatattaccg
      961 gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct ggaagcgaaa ttaattggtc
     1021 agggcgacga cccatggggt ggtaaacgtg caggcttcga ggccgaaggc aaaattaagc
     1081 tcaaggactt caatatcaag acagatttag gtccagcttc tcaggaagtg gatctgatta
     1141 tttcagtgga aggcgtacag cagaagtaag cgtgaaatgt tgccggatgg cacgcatccg
     1201 gcaatgatat ttattctgtt ggatcaggaa tgcccagtgt tgtattcaga cgtccacgag
     1261 acttattaaa gatcttattg ccattttcac gtcctgcccg acgccgtcgc tgttcttctg
     1321 gcggtaacgc gctttcttcg cagcaaatct cactacaaca acctttgtat ttttccgcgc
     1381 atactggaca ctgaataaac agcaggtggc agccatcatt tttacagttg gtatggctgt
     1441 cgcacggcgc gccgcactgg tggcaatgcg cgataatctc atccgatata cgttcgccca
     1501 tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac cggcaagccc tgctcgcgcg
     1561 ccttacgggc gtattcaata attccgccct cgatatgcca gactttgttg aatccgttat
     1621 gtttcatcca ggcactggcc ttttcacaac gaatgccgcc ggtgcagtac atgacgattt
     1681 ttttatcttt atgtgcctgc atcatctcga ctgcttttgg cagctgctca cggaaggtat
     1741 ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc atactcatag tggttacgca
     1801 tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt cacttccgcc gcttgcagat
     1861 actcaccaac attgctggca tcaaagtgag gatcgtcaat accgtcggca acaatgcgat
     1921 cgcgtacctt catgcgcagt acccagaagg atttcccgtc atcatccaac gcgatattca
     1981 ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg cgcgcgaaat gtttcaacat
     2041 tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg cgccagatac actcgcccaa
     2101 aaacattcag cgcggtaaac agctgatata aagcgtcacg ggtcgcctta ggatcggcga
     2161 tgtggaaata cttgtaaaac gaaatggtgg ttcgcggttc gctctcaacc aacattttgg
     2221 cttttaacgc gtcgttggaa atgcggttgt gtaacactgg catggtgtac ggttcctgcg
     2281 agatgggaaa gtaaaaatcc gcggcatgat atagcaatta tcgataatta acatccacac
     2341 attttacgct acatttgcgc attaaaaatt atttgttatt tacaagcgcg gcaatttcgc
     2401 ccagtcttca gccacaaatt ttggttgcgg gcgaaaaaat gcgacaatac atacaattgc
     2461 ccgaataggt tgaaaaacag gattgatatg acgaatctac ccaagttctc caccgcactg
     2521 cttcatccgc gttattggtt aacctggttg ggtattggcg tactttggtt agtcgtgcaa
     2581 ttgccctacc cggttatcta ccgcctcggt tgtggattag gaaaactggc gttacgtttt
     2641 atgaaacgac gcgcaaaaat tgtgcatcgc aacctggaac tgtgcttccc ggaaatgagc
     2701 gaacaagaac gccgtaaaat ggtggtgaag aatttcgaat ccgttggcat gggactgatg
     2761 gaaaccggta tggcgtggtt ctggccggac cgccgaatcg cccgctggac ggaagtgatc
     2821 ggcatggaac acattcgtga cgtgcaggcg caaaaacgcg gcatcctgtt agttggcatc
     2881 cattttctga cactggaact gggcgcgcgg cagtttggta ttcaggaacc ggggattggc
     2941 gtttaccgcc cgaacgataa tccactgatt gactggctac aaacctgggg ccgtttgcgc
     3001 tcaaataaat cgatgctcga ccgcaaagat ttaaaaggca tgattaaagc cctgaaaaaa
     3061 ggcgaagtgg tctggtacgc accggatcat gattacggcc cgcgctcaag cgttttcgtc
     3121 ccgttatttg ccgttgagca ggctgcgacc acgaccggaa cctggatgct ggcacggatg
     3181 tccggcgcat gtctggtgcc cttcgttcca cgccgtaagc cagatggcaa agggtatcaa
     3241 ttgattatgc tgccgccaga gtgttctccg ccactggatg atgccgaaac taccgccgcg
     3301 tggatgaaca aagtggtcga aaaatgcatc atgatggcac cagagcagta tatgtggtta
     3361 caccgtcgct ttaaaacacg cccggaaggc gttccttcac gctattaaat ctcccctgcc
     3421 ggatgcttca gaatggcatc cggcattacc acagcaaatc cccctgattt agcgataaaa
     3481 gctctctgga ttgcgccccc tggaagtcgg gcgcataatt agtgtgctta tcttttcttc
     3541 ttatgttcac cgcgcctggc gcaccaacag cggattgcta tgtcaccctg tggaaatgac
     3601 acccctataa actggaaacg aaacctgatc gtcgcctggc taggctgttt tcttaccggt
     3661 gccgccttca gtctggtaat gcccttctta cccctctacg ttgagcagct tggcgttacc
     3721 ggtcactccg ccctgaatat gtggtccggt attgtcttca gtataatacc ttcctaaata
     3781 cactacctga gagaagagat gaaaagagct atcagctaca tgaggttttc aactgagaag
     3841 cagataaaag gggactctat acgaagacag tctaagctgg tcactgactg gttatcaaag
     3901 aaccctga
//
LOCUS       DAEEKQ010000266         3864 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.271, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000266 DAEEKQ010000000
VERSION     DAEEKQ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3864
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.271"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..1803
                     /gene="fimD"
                     /locus_tag="KLK57_004085"
                     /pseudo
     CDS             1..1803
                     /gene="fimD"
                     /locus_tag="KLK57_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="outer membrane usher protein FimD"
     gene            1816..2823
                     /gene="fimH"
                     /locus_tag="KLK57_004086"
     CDS             1816..2823
                     /gene="fimH"
                     /locus_tag="KLK57_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HBE0894306.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNVFN
                     STNNQPGQIVVLPEKSGWVGVSAICPLGTLVNYTYRSYVTNFIVQETIDNYKYMQLND
                     YLLGAMSLVDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIRPFINMV
                     EIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFGDIGASL
                     FSAAGPGNRPAGVMPQTKSIAIKCTNVAAQAYLTMRLEASAVSGQAMVSDNQDLGFIV
                     ADQNDTPITPNDLNSVIPFRLDAAAAANVTLRAWPISITGQKPTEGPFSALGYLRVDY
                     Q"
     gene            2834..3349
                     /gene="sfmF"
                     /locus_tag="KLK57_004087"
     CDS             2834..3349
                     /gene="sfmF"
                     /locus_tag="KLK57_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HBE0894307.1"
                     /translation="MRRVLFSCFCGLLWSSSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTVAIELRNSDRSRLALGEASPTEEVDANGNVTLNFFANYRALASGVQPGVE
                     KADAIFMINYN"
     gene            complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="KLK57_004088"
     CDS             complement(3352..>3864)
                     /gene="fimZ"
                     /locus_tag="KLK57_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HBE0894308.1"
                     /translation="TIDYLRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLS
                     SKSECFYAGRAIQAGANGFVSKCNDQNDIFHPVQMILSGYTFFPSETLNYIKSNKCST
                     NSSTVTVLSNREVTILRYLVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIVEL
                     IDYAKLYELI"
BASE COUNT          988 a          846 c          959 g         1071 t
ORIGIN      
        1 tggtgtacgg ctttattctt ccgataatat gtatccagat agccagcaag ggtttgcccc
       61 aacggtacgt gggattgccc gtacggcggc ccagctaacg attcggcaaa atggttttat
      121 tatctatcaa agctatgttt cccccggtgc ttttgaaatt actgatttgc acccgacatc
      181 ttcaaatggc gatctggatg tcaccatcga cgagcgcgat ggcagaatta cacaattccg
      241 tattcaacag tgccgatttt acaacgcgaa gggcgtttca aatttgacct gacggcgggc
      301 gattttcgta gcggtaatag tcagcaatca tcacctttct tttttcaggg cacggcactc
      361 ggtggtttac cacaggaatt tactgcctac ggcgggacgc aattatctgc caattacacc
      421 gcctttttat tagggctggg gcgcaacctc gggaactggg gcgcagtgtc gctggatgtg
      481 acccatgcgc gcagccagtt agccgacgac agtcgtcatg agggggattc cattcgcttc
      541 ctctatgcga aatcgatgta caccttcggc accaattttc agttaatggg ttaccgctat
      601 tcgacacaag gtttttatac ccttgatgat gttgcgtatc gtcgaatgga ggggtacgaa
      661 tatgattacg attacgacgg tgagcatcgc gatgaaccga taatcgtgaa ttaccacaat
      721 ttacgcttta gccgcaaaga ccgtttgcag ttaaatattt cacaatcact taatgacttt
      781 ggctcgcttt atatctctgg tacccatcaa aaatactgga atacttcgga ttcagatacc
      841 tggtatcagg tggggtatac cagcagctgg gttggcatca gttattcact ctcattttcg
      901 tggaatgaat ctgtagggat ccccgataac gaacatattg tcggacttaa tgtttcagtg
      961 cctttcaatg ttttgaccaa acgtcgctac acccgggaaa atgcgctcga ccgcgcttat
     1021 gcctccttga acgctaaccg taacagcaac gggcaaaata gctggctggc aggcgttggt
     1081 gggaccttac tggaaggcca caacctgagt tatcacgtaa gccagggcga tacctcgaat
     1141 aatgggtaca cgggcagcgc cacggcaaac tggcaggcca cttacggtac gctgggggtc
     1201 gggtataact acgaccgcga tcaacatgac gttaactggc agctgtctgg cggtgtggtc
     1261 gggcatgaaa atggcataac actgagccag cctttagggg ataccaatgt tttgattaaa
     1321 gcgcttggcg caggcggtgt acgcattgaa aatcaaactg gcattttaac cgactggcgc
     1381 ggctatgcgg tgatgccgta tgccacggtt tatcggtata accgtatcgc gcttgatacc
     1441 aatacgatgg ggaattccat cgatgttgaa aaaaatatta gcagcgttgt gccgacgcaa
     1501 ggcgcgttgg ttcgtgccaa ttttgatacc cgcataggcg tgcgggcgct cattaccgtt
     1561 acccagggcg gaaaaccggt gccgtttgga tcactggtac gggaaaacag taccggaata
     1621 accagtatgg tgggtgatga cgggcaagtt tatttaagtg gtgcgccatt gtctggtgaa
     1681 ttactggttc agtggggaga cggcgcgaac tcacgctgca ttgcgcacta tgtattgcca
     1741 aagcaaagct tacagcaagc cgtcactgtt atttcggcag tttgcacaca tcctcgctca
     1801 taaaggaaat tatcaatgaa gataatctgc agattattat tggcaatggc atgtttgtgt
     1861 ctggcaaaca tatcctgggc tactgtttgt gcaaatagta ctggcgtagc agaagatgaa
     1921 cactatgatc tctcaaatgt ctttaatagc accaataacc agccagggca gattgttgtt
     1981 ttaccggaaa aatccggctg ggtaggtgtc tcagcaattt gtccactcgg cacgctggtg
     2041 aattatacat accgtagtta tgtcactaac tttattgttc aggaaactat cgataattat
     2101 aaatatatgc aattaaatga ttatctatta ggtgcgatga gtctggttga tagtgtgatg
     2161 gatattcagt tccccccgca aaattatatt cggatgggaa cagatcctaa cgtttcgcaa
     2221 aaccttccgt tcggggtgat ggattctcgt ttgatatttc gtttaaaggt tattcgtccc
     2281 tttattaaca tggtggagat ccccagacag gtgatgttta ccgtgtatgt gacatcaacg
     2341 ccttacgatc cgttggttac acctgtttat accattagtt ttggtggccg ggttgaagtg
     2401 ccgcaaaact gcgaattaaa tgccgggcag attgttgaat ttgattttgg tgatatcggc
     2461 gcatcgttat ttagtgcggc agggccgggg aatcgaccag ctggcgtcat gccgcaaacc
     2521 aagagcattg cgatcaaatg tacgaatgtt gctgcgcagg cttatttaac aatgcgtctg
     2581 gaagccagtg ccgtttctgg tcaggcgatg gtgtcggaca atcaggattt aggttttatt
     2641 gtcgccgatc agaacgatac gccgatcacg cctaacgatc tcaatagcgt tattcctttc
     2701 cgtctggatg cagctgcggc agccaatgtc acacttcgcg cctggcctat cagtattacc
     2761 ggtcaaaaac cgaccgaagg gccgtttagc gcgctggggt atttacgcgt cgattatcaa
     2821 tgaggtacgg agaatgagaa gagtactctt tagctgtttc tgcgggttac tgtggagttc
     2881 cagtggatgg gcagctgacc ctttgggaac gattaatatc aatttgcacg gtaacgttgt
     2941 tgatttctcc tgtaccgtaa acactgcgga tattgataag acggtagatt taggcagatg
     3001 gcctacgaca caattactga acgctggcga taccacggca ctcgtccctt ttagcctgcg
     3061 gctggaggga tgtcctccgg gttccgttgc gattttattt acgggaacgc cggcatccga
     3121 taccaacctg ctggctctgg atgatcccgc aatggcacaa accgtcgcca tcgaattacg
     3181 taatagcgat cgctcccggc tcgcactggg ggaggcgagc ccgactgagg aagtagatgc
     3241 aaatggcaat gtcacactaa acttttttgc caattatcga gcgttagcca gcggtgttca
     3301 gccaggtgtg gagaaagcgg atgcgatatt tatgatcaat tataattaat attatattaa
     3361 ttcgtataat ttggcgtagt cgataagctc tacaattgaa tgcaaaccta gtttgccata
     3421 aatattagat ttatgcgcac taactgtttt attgctaagt aataacttat cggcaatttc
     3481 tttattagat aatccgctaa ccagataacg taatatggtc acttcacgat tagatagcac
     3541 agtgaccgtt gaactattcg tactacattt attgcttttt atatagttga gcgtttcgct
     3601 aggaaaaaac gtgtatccgg agaggatcat ctgaacggga tgaaaaatat cattctgatc
     3661 attgcattta ctgacaaaac cgttagcacc agcttgtatc gctctgccag cataaaagca
     3721 ttctgatttc gatgataaaa ataacacttt cactgtgctc tggatttgtt tgatcctttt
     3781 caggaaggta aaaccgtctg ttccgggcaa gtctatatcc ataatgatta aatcaacagg
     3841 acgggttcgg agataatcga tggt
//
LOCUS       DAEEKQ010000267         3853 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.272, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000267 DAEEKQ010000000
VERSION     DAEEKQ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3853
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.272"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            115..510
                     /gene="caiF"
                     /locus_tag="KLK57_004089"
     CDS             115..510
                     /gene="caiF"
                     /locus_tag="KLK57_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HBE0894309.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCAVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(719..1309)
                     /gene="caiE"
                     /locus_tag="KLK57_004090"
     CDS             complement(719..1309)
                     /gene="caiE"
                     /locus_tag="KLK57_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HBE0894310.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="KLK57_004091"
     CDS             complement(1315..2100)
                     /gene="caiD"
                     /locus_tag="KLK57_004091"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HBE0894311.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFDGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="KLK57_004092"
     CDS             complement(2209..3762)
                     /gene="caiC"
                     /locus_tag="KLK57_004092"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HBE0894312.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTTNLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATVTECIPMMIRTL
                     MAQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
BASE COUNT          909 a         1047 c          941 g          956 t
ORIGIN      
        1 ttatcgacgg tgaagttgca tactatcgat atatccacaa ttttaatatg gccttgttta
       61 attgcttcaa aacgagtcat agccagactt ttaatttgtg aaactggagt tcgtatgtgt
      121 gaaggatatg ttgaaaaacc actctacttg ttaatcgccg aatggatgat ggctgaaaat
      181 cggtgggtga tagcaagaga gatctctatt catttcgata ttgaacacag caaggcggtt
      241 aataccctga cttatattct gtcggaagtc gcagaaataa gctgcgcagt taagatgatc
      301 cctaataagc tggaaggacg gggatgccag tgccagcgac tggtaaaagt ggtcgatatc
      361 gatgagcaaa tttacgcgcg cctgcgcaat aacagccggg ataaattagt cggcgtaaga
      421 aagacgccgc gtattcctgc cgttccgctc acggaactta accgcgagca gaagtggcag
      481 atgatgttgt caaagagtat gcgtcgttaa tgtcatttcg ttgataccac gcagcctggt
      541 tgctggatgc aatgctggcg catcttatcc agcctacggt tgctctccag ccgggttgga
      601 taaggcgttt atgccgcatc cgacagctat cgccggatgc gatgctggcg catcttatcc
      661 ggcctacggt tgctctccag cagagttgga taaggcgttt acgccgcatc cggcaagatc
      721 accgtttagg cgtcacatcc gtcgtcccct gcaaacgggg gcgattttcc tccatttgcc
      781 tcagtggctg tgtttcatgt aacgatgcat gacagcgccc gacaagatcc tgatactctt
      841 tggtattcaa ccgtttccag tgtaactcgt cgtcactaac actgcgtaca gcgcgggctg
      901 gcgtacccat caacaactgg cgtttctcgc cgtgaaagcc tgctttgaca aagctcatgg
      961 cggcaacaat gctctcttcg ccaatgaccg cgccatccat aatcacgctg ttcatcccga
     1021 ccaatgcatc gcgaccaatc acacaaccat gcaggatcgc tccgtgcccg atatggccgt
     1081 tttccccaac gatggtgtca gtgtcgcagt agccatgcat aatgcagcca tcctgaatat
     1141 tggctcccgc ttgcacgatc aaccgcccgt agtcaccacg cagtgaggcg agtgggccga
     1201 tgtagacacc ggctcccaca atcacatcgc caatcaagac ggcactggga tggacaaacg
     1261 ccgtcgggtg aaccaccgga attaacccct caaaggcgta atagctcacg gttgttaacg
     1321 tcctttccac accggatcgc gcttctcggc aaacgccagc ggcccttcaa tggcatcctc
     1381 cgaatgcaga accgacggat agtgtttcaa cacgccgctg cgaatatagc gatacgcttc
     1441 ttctaccggc atttcgctgg tggtgcggaa gatctctttc agcgccgcaa tcgccagcgg
     1501 ggcgctgtta accagctgct gcgccagttc gcgggcgtta tccatcagtt ccgcctggct
     1561 aaccacgcgg ttgactatcc cccaacgcag cgcctcttct gcgcccattc gtctgccggt
     1621 catcaccatt tcattgacga tggcaggcgg caggatcttc ggtagacgca gcacgccgcc
     1681 gctgtcagga acgatgccca gcttggcttc cggcagggcg aagctggcgt tatcggcaca
     1741 aacaataaaa tctgccgcca gcgccagttc gaaaccgccg tcaaaggcat agccgttcac
     1801 agctgcgata accggtttgt cgagattgaa aatttcggtt aatcccgcaa atccacccgg
     1861 gccaaagtca gcatccggtg cttcgccttc tgctgccgct tttaaatccc agcccgcgga
     1921 aaagaacttc tctccggcac cggtaataat ggcgacacgt aattgcggat cgtcacggaa
     1981 atttagaaat acttcgccca tttcaaagct ggtttttgca tcaatagcat tcgcttttgg
     2041 acgatcaagg gtaatttcca gaattgatcc attgcgggtc agatgtaatg attcactcat
     2101 tccttttctc catttttgct ttttcaggga cgacaacatc cctgcaaaaa atgcatattg
     2161 ttttatagcg tgattattag ttggcagggt agttccctgc tgtttcattt atttcagatt
     2221 ctttctaatt attttccccg agcaattacg tggcagatct tttctgatct ccagataaga
     2281 gggcacttta aatttcgcca tattttgttc gcagaagcgg aaaaattcct cttcgctcaa
     2341 tgtttcacct tcattcagca ccacaaatgc tttgatggct tcatcgcgaa tcgaatcttt
     2401 aatacccaca accacgatat cctgaatttt tggatgggtg gcgatgatat tttccagctc
     2461 cacgcaggag acattctcgc caccgcgttt gatcatattg cagcggcgat cgacgaaata
     2521 aaaaaagccc tcttcgtcgc agtatccggt atcgccggta tgtagccagc cgtcggcttc
     2581 cagcactttc gcagtggctt tcgggttgag aaaatactct ttgaagatgg ttttccctgg
     2641 tacgccttta atacagattt caccgatctc acctgccggg agcgggcgat tgtgatcgtc
     2701 gcggatctcc gcttcgtagc aaaaccccgc ccgaccaatc gacggccagc gtcgtttatc
     2761 gccagggcga tcgccgataa tgcccacaat ggtttccgtc atcccataag acgtcagcaa
     2821 gcgaacgccg aagcgttcac aaaacgcatc tttttcctgc tccgacaagt tgagataaaa
     2881 catcacttcc cgcaggcggt gttgccgatc gttcgctgaa ggcggctgcg ccatcaaggt
     2941 acggatcatc atcggaatac attcggtaac ggtggcgcgg tacttctgta cctgtcccca
     3001 gaaggcgcgg gcgctgtatt tctcgaccag cacaaaggtg gccccggcag aaaacgccgc
     3061 catcgccgca gtacactggc aatcgatatg aaacgcaggc attaccgtca ggtagacgtc
     3121 atcgtcacgc agtgcacact gccaggcgga gtaatatcca gcgaagcgca ggttgtaatg
     3181 ggtaatcacc acacctttcg gtcgggaggt ggtgccggag gtgaagagaa tttccgccgt
     3241 atcgtcagtc gatagcggcg gtgcatagca caaggtggca ggttgttgat ttttcagttg
     3301 agtaaacgaa ctcacgccat catcagcggg aagtgccaca tctgtcaggc aaatgtgccg
     3361 caattgagtg gcatcttcct gctgaatctg ttgatacata ggatagaatt gcgcactggt
     3421 caccagcagg cacgcctggc tattttgcag gatccacgcg ctttcctcgc gcaacaggcg
     3481 ggcgttaatc ggcaccataa tcgcgccaat ttttgccagc ccgaaccagc aaaagataaa
     3541 ttccgggcag ttgtcgagat gtagagcaac cttgtcgcct ttgcgaatcc ccagcgtata
     3601 aaacaggttt gtcgtgcggt taatctcctg atttaactca agataactat accggttaac
     3661 gactccgccg ctggattcac aaatcagcgc cgttttatga ccgtaaacgt ccgccagatc
     3721 gtcccacatt tgacgtagat gttgtccgcc aatgatatcc attgcacctc tatccatttt
     3781 tgttcgtttg ttattgggcg ggcgctagtc aggcaagccg actgacgcca cgcgtttagt
     3841 cctcaacttt ggc
//
LOCUS       DAEEKQ010000268         3832 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.273, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000268 DAEEKQ010000000
VERSION     DAEEKQ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.273"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..266)
                     /locus_tag="KLK57_004093"
     CDS             complement(<1..266)
                     /locus_tag="KLK57_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HBE0894313.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRELTDAGGFLDISL"
     gene            353..760
                     /gene="nudG"
                     /locus_tag="KLK57_004094"
     CDS             353..760
                     /gene="nudG"
                     /locus_tag="KLK57_004094"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HBE0894314.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFADGKVELDES
                     QQQALVRELNEELGIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            complement(726..998)
                     /locus_tag="KLK57_004095"
     CDS             complement(726..998)
                     /locus_tag="KLK57_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HBE0894315.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            1234..2577
                     /gene="gdhA"
                     /locus_tag="KLK57_004096"
     CDS             1234..2577
                     /gene="gdhA"
                     /locus_tag="KLK57_004096"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HBE0894316.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQIQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(2694..3733)
                     /locus_tag="KLK57_004097"
                     /pseudo
     CDS             complement(2694..3733)
                     /locus_tag="KLK57_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
BASE COUNT          997 a          931 c          917 g          987 t
ORIGIN      
        1 tcaagagaaa tatcaagaaa gccgcccgcg tcggtaagct ctctgcgccg cgccagcgca
       61 ccgtccagac catcaagcag cctgttcaac aaaatgacga ctagcgccgc cagataccag
      121 cccagcgcca gaaacggcag agccagcacg ccgatggcaa atcccactaa tgttaaacca
      181 tctggcgtaa tgcccggttt atcaagaacc cgcacgcact gatgcaacaa cggtttaatc
      241 cggggatgaa gatggcggtc tagcacaggc actccttaaa tataaagcct ttctgattga
      301 gcaacagtgc ggatattatg gcatttttcg cttatctgcc cgtgtgtaat ttatgaaaat
      361 gattgaagtt gttgccgcca tcattgaacg tgatggcaaa attttactcg cgcaacgccc
      421 cgcccagagc gatcaggcgg gattatggga gtttgccgat ggtaaagtcg agctggatga
      481 aagccagcag caggcactgg tgcgtgagtt aaacgaagaa ttgggcatcg aagcaactgt
      541 gggtgagtat gttgccagcc atcagcgaga agtttcgggc cggattatcc atcttcatgc
      601 ctggcacgta cccgacttcc acgggacgtt acaggcacat gaacatcagg cgctggtctg
      661 gtgctcacct gaagaggcgc tgcaatatcc gctggcccct gctgacattc cactattaga
      721 ggcgtttatg gctttacgcg ccgccagagc agcggattag tgctaagggt tttgtcatcc
      781 cgctggcatt gcagcagtat tccttcggct ttaattaccg ccccttcaga ataattttga
      841 tcctgataaa cgcagcactg agtacagggc tgcgctgact gcccgcctga actgaacact
      901 tctggcggta cgtttacctc cacgtccgga cgataatgcg ggttagccag tgcaattaat
      961 ggaaatgcta atactacggc gaacaatgct cgactcacag ggaactcctt aacgttattg
     1021 tctctgcaac tgataacggt agccgggtgg caaaacttta gcgtctgagt tatcgcattt
     1081 ggttatgaga ttactctcgt tattaatttg ctttcccggg tcattttttt cttgcttacc
     1141 gtcacattct tgatggtata gtcgaaaact gcaaaagcac atgacataaa caacataagc
     1201 acaatcgtat taatatataa gggttttata tctatggatc agacatattc tctggagtca
     1261 ttcctcaacc atgtccaaaa gcgcgacccg aatcaaaccg agttcgcgca agccgttcgt
     1321 gaagtaatga ccacactctg gccttttctt gaacaaaatc caaaatatcg ccagatgtca
     1381 ttactggagc gtctggttga accggagcgc gtgatccagt ttcgcgtggt atgggttgat
     1441 gatcgcaacc agatacaggt caaccgtgca tggcgtgtgc agttcagctc tgccatcggc
     1501 ccgtacaaag gcggtatgcg cttccatccg tcagttaacc tttccattct caaattcctc
     1561 ggctttgaac aaaccttcaa aaatgccctg actaccctgc cgatgggcgg tggtaaaggc
     1621 ggcagcgatt tcgatccgaa aggaaaaagc gaaggtgaag tgatgcgttt ttgccaggcg
     1681 ctgatgaccg agctttatcg tcatctgggc gcggataccg acgttccggc aggtgatatc
     1741 ggcgttggtg gtcgtgaagt cggctttatg gcggggatga tgaaaaagct ctccaacaat
     1801 accgcctgcg tcttcaccgg taagggcctt tcatttggcg gtagtcttat tcgcccggaa
     1861 gctaccggct acggtctggt ttatttcaca gaagcaatgt taaaacgcca cggtatgggt
     1921 tttgaaggga tgcgcgtttc cgtttctggc tccggcaacg tcgcccagta cgctatcgaa
     1981 aaagcgatgg aatttggtgc tcgtgtgatc actgcgtcag actccagcgg cactgtagtt
     2041 gatgaaagcg gattcacgaa agagaaactg gcacgtctta tcgaaatcaa agccagccgc
     2101 gatggtcgag tggcagatta cgccaaagaa tttggtctgg tctatctcga aggccaacag
     2161 ccgtggtctg taccggttga tatcgccctg ccttgcgcta cccagaacga actggatgtt
     2221 gacgccgcgc atcagcttat cgccaatggc gttaaagccg tcgctgaagg ggcaaatatg
     2281 ccgaccacca tcgaagcgac tgaactgttc cagcaggcag gcgtactgtt tgcaccgggt
     2341 aaagcggcta atgctggcgg tgtagcaacg tcgggcctgg aaatggcaca aaacgctgcg
     2401 cgcctgggct ggaaagccga gaaagttgac gcacgtttgc atcacatcat gctggatatc
     2461 caccatgcct gtgttgagca tggtggtgaa ggtgagcaaa ccaactacgt gcagggcgcg
     2521 aacattgccg gttttgtgaa ggttgccgat gcgatgctgg cgcagggtgt gatttaagtt
     2581 gtaaatgcct gatggcgcta cgcttatcag gcctacaaat gggcacaatt cattgcagtt
     2641 acgctctaat gtaggccggg caagcgcagc gcccccggca aaatttcagg cgtttatgag
     2701 tatttaacgg atgatgctcc ccagggaaca tttcttatgg gccaacggca tttcttactg
     2761 tagtgctccc aaaattgctt gtcgtaacga taacacgctt caagttcagc atccgttaac
     2821 tttctgcgat agcagcagat atgccagtaa agaaacccca ttagactatt tttttgataa
     2881 tcttcttcgc tttcgaacaa ctcgtgcgcc tttcgagaag caagcattat ataatgccag
     2941 gccagttctt cttcaattgt cccgttttga aaagctgtgc ttgatatcga ggtcatccat
     3001 gataattccg ccgcccatat tagcttcgcc gaggatttac cggagcgatt attagcgcaa
     3061 tcagagatat agtctgaggg aaaaacagca aatttattca acaaggcgat aacctgctct
     3121 ggggcttcct ccatgtttgc tttaaaggta ttggctccat ggtcgccaga aagaaaatgc
     3181 tccattaggc acaataactt tcgctatctt cgatacccca ttgattctct aaagactcgc
     3241 gtcttttact gatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt
     3301 taccaggttc aggctttcgc ggccagaact ctagcgtttc agaccattgc ttatgataga
     3361 atcgataagg tgcgatcaat tgtagcgcct gtaacttctc gatactgagc ggctcaatac
     3421 ctttagcctg ataataatgc agttgttctt tttttgcttt aaaaccggcc cgaacaataa
     3481 gccccatcat aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat
     3541 tcaaaccgtt ggatgttatc gctgcgaaca caaacattac agcgacaaca catgttaaat
     3601 aaaaccccca cttacaaagc agcatggcct tattttcttt aatcatccgt tcaaaattac
     3661 tattaaatat ttcccagcca ttaaaagaat acttctcgct cccaggatgg ttttgtaata
     3721 aaactttttt catcacattc cctgttatta catactttag aggcgataat tttatccatg
     3781 caaaaaaaat atccaacgaa ttttcttgat ccgctggata tttcagaata tg
//
LOCUS       DAEEKQ010000269         3802 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.274, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000269 DAEEKQ010000000
VERSION     DAEEKQ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.274"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            73..732
                     /locus_tag="KLK57_004098"
     CDS             73..732
                     /locus_tag="KLK57_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HBE0894317.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLTEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            836..1810
                     /gene="ghrB"
                     /locus_tag="KLK57_004099"
     CDS             836..1810
                     /gene="ghrB"
                     /locus_tag="KLK57_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HBE0894318.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLHATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(1860..2570)
                     /locus_tag="KLK57_004100"
     CDS             complement(1860..2570)
                     /locus_tag="KLK57_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HBE0894319.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            3004..3294
                     /locus_tag="KLK57_004101"
     CDS             3004..3294
                     /locus_tag="KLK57_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HBE0894320.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            3575..3787
                     /gene="cspA"
                     /locus_tag="KLK57_004102"
     CDS             3575..3787
                     /gene="cspA"
                     /locus_tag="KLK57_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HBE0894321.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
BASE COUNT         1001 a          949 c          937 g          915 t
ORIGIN      
        1 tcatgttcac gcggcataat ctcccgccac ggaacccgtg gcaaagaata aaaaggttat
       61 taaggattaa caatgaagaa acgtgtttat cttattgccg ccgtagtgag tggcgctctg
      121 gcggtatctg gctgcacaac taacccttac accggcgaac gcgaagcagg caaatctgct
      181 atcggcgcag gtctgggctc tctcgtgggc gcgggtattg gtgcgctctc ttcttcgaag
      241 aaagatcgcg gtaaaggcgc gctgattggc gcagcagcag gcgcagctct gggcggcggc
      301 gtgggttatt acatggatgt gcaggaagcg aagttgcgtg acaaaatgcg cggcactggt
      361 gttagcgtaa cccgcagcgg ggataacatt atcctcaata tgccgaacaa tgtgaccttc
      421 gacagcagca gcgcgaccct gaaaccggca ggcgctaaca ccctgaccgg cgtggcaatg
      481 gtgctgacag agtatccgaa aacggcggtt aacgtgattg gttataccga cagcacgggg
      541 ggtcacgacc tgaacatgcg tctctcccag cagcgtgcgg attccgttgc cagcgcgttg
      601 atcacccagg gcgtggacgc cagccgcatc cgtactcagg gccttggccc ggctaaccca
      661 atcgccagca acagcaccgc agaaggtaag gcgcaaaacc gccgtgtaga aattacctta
      721 agcccgctgt aatccctttc atgccaggtg atgcagaaat cacctggcat tcttcagttt
      781 tccttcatca tatttcaggc taaggtgatc gccttatcag tgaatggaga gaagcatgaa
      841 gccgtccgtt atcctctaca aagccttacc tgatgattta ctgcaacgcc tgcaagagca
      901 tttcaccgtt caccaggtgg caaacctcag cccacaaacc gtcgaacaaa atgcagcaat
      961 ttttgccgaa gctgaaggtt tactgggttc aaacgagaat gttgatgccg cattgctgga
     1021 aaaaatgccg aaactgcatg ccacatcaac gatctccgtc ggctatgaca attttgatgt
     1081 cgatgcgctt accgcccgaa aaattctgct gatgcacacg ccaaccgtat taacagaaac
     1141 cgtcgccgat acgctgatgg cgctggtgtt gtctaccgct cgtcgggttg tggaggtagc
     1201 agaacgggta aaagcaggcg aatggaccgc gagcataggc ccggactggt acggcactga
     1261 cgttcaccat aaaacactgg gcattgtcgg gatgggacgg atcggtatgg cgctggcaca
     1321 acgtgcgcac tttggcttca acatgcccat cctctataac gcgcgccgcc accataaaga
     1381 agcagaagaa cgcttcaacg cccgctactg cgatttggat acactgttac aagagtcaga
     1441 tttcgtttgc ctgatcctgc cgttaactga tgagacgcat catctgtttg gcgcagaaca
     1501 attcgccaaa atgaaatcct ccgccatttt cattaatgcc ggacgtggcc cggtggttga
     1561 cgaaaatgca ctgatcgcag cattacagaa aggggaaatt cacgccgccg ggcttgatgt
     1621 cttcgaacaa gagccactgt ccgtagattc gccgttgctc tcaatggcca acgtcgtcgc
     1681 agtaccgcat attggatctg ccacccatga gacgcgttat ggcatggccg cctgtgccgt
     1741 ggataattta attgatgcgt tacaaggaaa ggttgagaag aactgtgtga atcctcacgt
     1801 cgcggactaa gccgcgactg cgtgaagtaa agcccgataa tcgctcgggc ttttactctt
     1861 tattgggttg cagtaactgc tgtagtccag gcctgattaa acgcctgatg ttgtgccggt
     1921 aatggcgcaa tcagtttgtt atattcactt gcctgctgtg aagtcgggaa ctggatgcca
     1981 tttgcgacaa agctcacctg agtaccttgc tgagcaatgt agtctccaac catcaccagt
     2041 tgttgcgtga aagtttgtgc cgccggaatc aacggttgca atgcatcagc tggcgtcgtt
     2101 accactttgg tgaatgcctg atcaaatacc ggtttcaggt catcgctctg ttttagcgca
     2161 gagtgcgcag catcggcctg caacttcgca ttttgcagtt gttgcgccag cacacccagc
     2221 gaaccattca tttcacgcag cggaccgctt tgcgtaacgt aatcctgcgg tacgcgaatt
     2281 gcgttaacgc tgtctacgac cgggcgcaga ccagaatcca tcgcctgatt cacctgctga
     2341 gaataaccat acagaatcgc gtaatcagag acaaaaggac caaactgttt tttctgatcg
     2401 gcagtcaggg ttggtagacg ttcaccgcta cgcatcactg tattctgcag gaagtcgata
     2461 aacgctttac gctgatcgcc ttctttatca aaacacccac tcaggctaac taccattaat
     2521 aacgccgcaa gaggcgcaaa ccagcgagag caggactttc ctgtcgccat tttcttactc
     2581 ctttcaccca aaaaaagcgc acaacgacac acgcgtgtct gacgtgacaa ggatagtcca
     2641 ggacaggctt gcaggatacc ctttctacgt aatcttcctg gtaaaatcat ggagaaaatg
     2701 aaacaaggtt aatttgttgt ttcattgtta aaaaatgata gtgccgttgc cttttttgta
     2761 gccagagatt tacgccttcg cagaaggtta gattttcgaa atctttacaa aacaggagtg
     2821 gtaagagata tatcggcatt tatcagatcg ttaggtggct ataagtcacg ctaaatgaca
     2881 ggctgaatcg aatcatagcc agagcatgcc ctgacttcac cccgctgtgt ctgcttttcc
     2941 cgactattct taatgagctt cgatgcaatt cacgatcccg cagtgtgatt tgaggagttt
     3001 tcaatggaat ataaagatcc aatgcatgag ctgttgagca gcctggaaca gattgttttt
     3061 aaagatgaaa cgcagaaaat taccctgacg cacagaacaa cgtcctgtac cgaaattgag
     3121 cagttacgaa aagggacagg gttaaaaatc gatgatttcg cccgggtttt gggcgtatca
     3181 gtcgccatgg taaaggaatg ggaatccaga cgcgtgaagc cttcaagtgc cgaactaaaa
     3241 ttgatgcgtt tgattcaagc caacccggca ttaagtaagc agttgatgga atagactttt
     3301 atccacttta ttgctgttta cggtcctgat gacaggaccg ttttccaacc gattaatcat
     3361 aaatatgaaa aataattgtt gcatcacccg ccaatgcgtg gcttaatgca catcaacggt
     3421 ttgacgtaca gaccattaaa gcagtgtagt aaggcaagtc ccttcaagag ttatcgttga
     3481 tacccctcgt agtgcacatt cctttaacgc ttcaaaatct gtaaagcacg ccatatcgcc
     3541 gaaaggcaca cttaattatt aaaggtaata cactatgtcc ggtaaaatga ctggtatcgt
     3601 aaaatggttc aacgctgaca aaggcttcgg cttcatcact cctgacgatg gctctaaaga
     3661 tgtgttcgta cacttctctg ctatccagaa cgatggttac aaatctctgg acgaaggtca
     3721 gaaagtgtcc ttcaccatcg aaagcggcgc taaaggcccg gcagctggta acgtaaccag
     3781 cctgtaatct ctgcttaaaa gc
//
LOCUS       DAEEKQ010000270         3798 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.275, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000270 DAEEKQ010000000
VERSION     DAEEKQ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3798
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.275"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(99..908)
                     /gene="proC"
                     /locus_tag="KLK57_004103"
     CDS             complement(99..908)
                     /gene="proC"
                     /locus_tag="KLK57_004103"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HBE0894322.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            1028..1486
                     /locus_tag="KLK57_004104"
     CDS             1028..1486
                     /locus_tag="KLK57_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HBE0894323.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            1669..2193
                     /gene="aroL"
                     /locus_tag="KLK57_004105"
     CDS             1669..2193
                     /gene="aroL"
                     /locus_tag="KLK57_004105"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HBE0894324.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            2243..2434
                     /gene="yaiA"
                     /locus_tag="KLK57_004106"
     CDS             2243..2434
                     /gene="yaiA"
                     /locus_tag="KLK57_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HBE0894325.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            2692..3369
                     /locus_tag="KLK57_004107"
     CDS             2692..3369
                     /locus_tag="KLK57_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HBE0894326.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            3441..3725
                     /gene="ppnP"
                     /locus_tag="KLK57_004108"
     CDS             3441..3725
                     /gene="ppnP"
                     /locus_tag="KLK57_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HBE0894327.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
BASE COUNT          951 a          904 c          990 g          953 t
ORIGIN      
        1 tcgtgagtgg ttgaaatcgg cagatttggc gctttacaaa gcaaagaaag ccggacgtaa
       61 ccgcaccgaa gtggcggcct gacgtccggc gaaagtcatc aggatttgct gagtttttct
      121 gatttttcca tacacttcgt catcgcttcg atcactgcgg cacggaagcc tttctcttcc
      181 agtacgcgta ccgcttcaat ggtggtgcct cccggtgagc agaccatatc tttcagtgcc
      241 cccggatgtt ctcccgtttc cagcaccatt tttgcggaac ccattaccgc ctgagcggca
      301 aatttatacg cctgggcgcg tggcatcccg cccagcacgg cggcgtcggc catcgcttcg
      361 ataaacataa atacgtaggc tggcgaagaa ccgctcacac cgaccaccgg gtggatcatc
      421 ggctcagcaa ttacttccgc ttcgccaaag cagcggaaaa tattcagcac atcagcggta
      481 tcttcggggg ttaccagcgc gtttggcgtt acggaggtca tcccggcatt aaccagcgcg
      541 ggagtgttcg gcatggcgcg gataattttc cggtcatggc ccagcgcgcg ggcaagctgg
      601 tcgagcgtga cacctgcagc aatagaaacg accagagagt ctttattcag gctggaggtg
      661 atttcgctaa gcactttaat catgatgcca ggtttaacgg cggcaaaaat gatgtcggcg
      721 atttgcgcca cttcttgcgc cgattctgcg gcgttgatgc cgaactggtc atgcagggcg
      781 gcgactttat ccggggaggg ggtgtatacc cagatttgcc ctggaagcac ctgaccgctg
      841 gcaatcagac cgccgagaat ggcttttccc atattgccgc agccaataaa accgattttc
      901 ttttccattg cctcactcct gccgtgaaat tcattgtttt gataatcgct ggcagaagca
      961 taaacagaac tatgccggaa ggcaaaagcg cgacacaata gaggattacc caacaaagga
     1021 tgactttatg acaatttggg tggatgccga cgcgtgtccc aatgtaatta aagagatttt
     1081 gtatcgcgcg gcggaacgta tgcagatgcc gctggtactg gtagcaaacc agagtttacg
     1141 cgtgccgcca tcgcgattta ttcgtacgct gcgcgtcgcg gcaggtttcg acgttgccga
     1201 taacgaaatt gtccggcagt gtgaagcggg cgatttggtg atcaccgcag atataccttt
     1261 ggctgctgaa gccatcgaga aaggcgcagc ggcgcttaat ccgcgcggcg aacgttacac
     1321 gccagcgacc attcgtgagc gcctgacgat gcgcgatttt atggatacct tacgtgccag
     1381 tgggatccag accggcggac cagatagcct ttcacaacgt gaccgccagg cctttgccgc
     1441 ggagctggag aagtggtggc tggaagtgca acgtagtcgt ggctaaatgt aatttattat
     1501 ttacacttca ttcttgaata tttattggta tagtaaggga tgtattgaga ttttcacttt
     1561 aagtggaatt ttttctttac aatcgaaatt gtactagttt gatggtatga tcgctattct
     1621 catgacaccg gctttcgccg cattgcgacc tattggggaa aacccacgat gacacaacct
     1681 ctttttctga tcgggcctcg gggctgtggt aaaacaacgg tcggaatggc ccttgccgat
     1741 tcgcttaacc gtcggtttgt cgataccgat cagtggttgc aatcacagct caatatgacg
     1801 gtcgcggaga tcgtcgaaag ggaagagtgg gcgggatttc gcgccagaga aacggcggcg
     1861 ctggaagcgg taactgcgcc atccaccgtt atcgctacag gcggcggcat tattctgacg
     1921 gaatttaatc gtcacttcat gcaaaataac gggatcgtgg tttatttgtg tgcgccagta
     1981 tcagtcctgg ttaaccgact gcaagctgca ccggaagaag atttacggcc aaccttaacg
     2041 ggaaaaccgc tgagcgaaga agttcaggaa gtgctggaag aacgcgatgc gctatatcgc
     2101 gaagttgcgc atattatcat cgacgcaaca aacgaaccca gccaggtgat ttctgaaatt
     2161 cgtagcgccc tggcacagac gatcaattgt tgattttaga gcgcctatac ttaacgttca
     2221 tcccgtgaaa taaggaagaa cgatgccaac gaaaccgcct tatcctcgtg aagcatatat
     2281 agtgacgatt gaaaaaggaa agccaggaca gacggtaacc tggtaccaac tcagagccga
     2341 tcatcctaaa ccagactcgt tgatcagtga acatccgacg gctcaggaag cgatggatgc
     2401 gaaaaaacgc tatgaggacc ctgacaaaga gtgaccgcat cagactgctc ggaagggatt
     2461 ctgagtgcca ctacaaggga tctgcgtcac atttttcata attcatgttt ttctaataat
     2521 tagaatatta aacaataaca atccattact ggaatcattt ggaatcttta cattatgccg
     2581 tgcacgtctg ctgctacgct ttttgtcatt tgtagcacaa gtaagtgtca gcagtggtgc
     2641 tccacacttg cccggtaatt aacgacgaaa gaaaagtaag gtggatgaac aatgagtgcg
     2701 tcgttggcga tcctgaccat cggcattgta cctatgcagg aggttttgcc gctcctgacg
     2761 gaatacattg acgaagataa tatttcccat catagcctgc tggggaagtt aagtcgtgaa
     2821 gaagtgatgg cggagtacgc gccagaagca ggcgaagaca ccattctcac attattaaat
     2881 gacaaccagc tggcccatgt ttcgcgtcgt aaagtggagc gagacctgca aggtgtggtt
     2941 gaagtgctcg ataatcaggg ttatgacgtc attttattaa tgagtacagc aaacattagt
     3001 agtatgactg cgcgtaatac gatctttctt gagccgtcgc gaatattgcc tccactggtt
     3061 tcctctattg ttgaagatca tcaggtgggg gttatcgttc cggttgagga gatgctgacc
     3121 gttcaggcgc aaaaatggca aattttgcag aaatcgccgg tattttcatt gggtaacccc
     3181 attcatgatt cagaacaaaa aatcattgat gccgggaaag aattactggc aaaaggggct
     3241 gatgtcatca tgctggattg tttaggattt caccaacgtc atcgcgattt actgcaaaaa
     3301 cagctcgatg ttcctgtctt gctgtctaac gtattgattg cacggctggc tgcggaatta
     3361 ctggtgtaat tttgcgtgac agccagcgcc tctggcccct atagtgaagt agatgttcaa
     3421 ctaccagaca gggccagttt atgcttcaaa gtaatgagta cttttccggc aaagtgaaat
     3481 caatcggctt ttccagcagc agcactggtc gcgccagcgt gggtgttatg gttgaaggcg
     3541 aatacacctt cagcaccgct gagccggaag agatgacggt aatcagtggc gcgctgaatg
     3601 tgttactgcc tgacgcgacc gactggcagg tgtatgaagc cggttcggtg tttaatgttc
     3661 ccggtcacag tgagtttcat ctgcaagttg ccgaacccac ctcttatctg tgccgctatc
     3721 tgtaattcct cgccttcccc ttgaacggga gggcattttt ctgaaatatc ctctctttaa
     3781 cccataataa tatttcct
//
LOCUS       DAEEKQ010000271         3755 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.276, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000271 DAEEKQ010000000
VERSION     DAEEKQ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3755)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3755)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3755
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.276"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..1360
                     /gene="dcuC"
                     /locus_tag="KLK57_004109"
     CDS             <1..1360
                     /gene="dcuC"
                     /locus_tag="KLK57_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HBE0894328.1"
                     /translation="GVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAIMGHKVLPSS
                     QASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMVVKLASKPLQ
                     YINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRGAAAAICASP
                     AAIILAPTSGDVVLAAQASEVSLIDFAFKTTLPISIAAIIGMAIAHFFWQRYLDKKEH
                     ISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVICMLIASILE
                     FLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQSLISIATSF
                     GSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAYLTIPMLQAS
                     NLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELMVPGTAAAVT
                     GK"
     gene            complement(1401..2081)
                     /gene="dpiA"
                     /locus_tag="KLK57_004110"
                     /gene_synonym="citB"
     CDS             complement(1401..2081)
                     /gene="dpiA"
                     /locus_tag="KLK57_004110"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HBE0894329.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISCTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2050..3708)
                     /gene="dpiB"
                     /locus_tag="KLK57_004111"
     CDS             complement(2050..3708)
                     /gene="dpiB"
                     /locus_tag="KLK57_004111"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HBE0894330.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHLNPEKIGYPMQFTKQGALEKGESYFITGKGAMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHHNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
BASE COUNT          857 a          985 c          910 g         1003 t
ORIGIN      
        1 tggggttgtg gttattgtgg gtgtagctcg ctacatcatt aaagggtatt ccgccactgg
       61 tgtgttattt gtcggtggcc tgttattgct gattatcagt gccattatgg ggcacaaagt
      121 gttaccgtcc agccaggctt caacaggcta cagcgccacg gatatcgttg aatacgttaa
      181 aatattacta atgagccgcg gcggcgacct cggcatgatg attatgatgc tgtgtggatt
      241 tgccgcttac atgacccata tcggcgcgaa tgatatggtg gtcaagctgg cgtcaaaacc
      301 attgcagtat attaactccc cttacctgct gatgattgcc gcctattttg tcgcctgtct
      361 gatgtctctg gccgtctctt ccgcaaccgg tctgggtgtt ttgctgatgg caaccctatt
      421 tccggtgatg gtaaacgttg gtatcagtcg tggcgcagct gctgccattt gtgcctcccc
      481 ggcggcgatt attctcgcac cgacttcagg ggatgtggtg ctggcggcgc aagcttccga
      541 agtgtcgctg attgacttcg ccttcaaaac gacgctgcct atctcaattg ctgcaattat
      601 cggcatggcg atcgcccact tcttctggca acgttatctg gataaaaaag agcacatctc
      661 tcatgaaatg ttagatgtca gtgaaatcac caccactgct cctgcgtttt atgccatttt
      721 gccgttcacg ccgatcatcg gtgtactgat ttttgacggt aaatggggtc cgcaattaca
      781 catcatcact attctggtga tttgtatgct gattgcctcc attctggagt tcctccgcag
      841 ctttaatacc cagaaagttt tctctggtct ggaagtggct tatcgcggga tggcagatgc
      901 gtttgctaac gtggtgatgc tgctggttgc cgctggggta ttcgctcagg ggcttagcac
      961 catcggcttt attcaaagtc tgatttctat cgctacctcg tttggttcgg cgagtatcat
     1021 cctgatgctg gtattggtga ttctgacaat gctggcggca gtcacgaccg gttcaggcaa
     1081 tgcgccgttt tatgcgtttg ttgagatgat cccgaaactg gcgcactctt ccggcattaa
     1141 cccggcgtat ttgactatcc cgatgctgca ggcgtcaaac ctgggtcgta ccctatcacc
     1201 cgtttctggc gtagtcgttg cggttgccgg gatggcgaaa atctcaccat ttgaagtcgt
     1261 aaaacgcacc tcggtgccgg tgcttgttgg tttggtgatt gttatcgttg ctacagagct
     1321 gatggtgcca ggaacggcag cagcggtcac aggcaagtaa atagtaatgc cggaggcaag
     1381 tttcctccgg ctttatcatg tcacccactg tggtatatgc gttgtggtct gccaactttg
     1441 ccgtgaacaa tttcagcaat aatcagatgg cggctggcgc aatattcaag ataacgcctg
     1501 gcagtggtgc agctgatggt cagtgcctgc gccaccgttt ctgccgtatg ttgcacacca
     1561 ggctctttaa acagttttcg caccgcgttt agcgtcaagg ggtcaatgcc ggtcggtagc
     1621 tcgtccttag gttcaccgcg agcataagca ttaaacatct catcaatttg cttctggctg
     1681 gcgctatcaa tactttccag catatgttta cgctggcgga aacgggttag cgtttgcccc
     1741 agccgttcat aggcaatggg cttaatgaga taatcaaata caccacaacg tacggcttca
     1801 gacaccgttt ccatatcgct ggctgcagtg gtaaacacca cgtcgccggg ataatgcgcc
     1861 tgcaccagtt catgcagtaa attaatccct ctaccgtcag gaagatagtt atcgagcaag
     1921 attagccccg gcttaaaacg ctcgatcatc attcgggcct gcgccagatt tcccgccagt
     1981 aatatctgac tgaatccggg aatgtgacga atatattccg catgcatctc tgccagcggc
     2041 gtttcgtcct caacgatcaa tagggttaat ggagctgtca ttaggtttca ctttcggaat
     2101 atagattgaa aataaggtac cgcagggatc attatcttcg agagtgataa caccaccgca
     2161 gcgcgttacg tagctggcaa tcaagtacaa cccaatgcca tgttcaccgg gctcgtcagc
     2221 acgcgtactg accccctgct caaatatttt gtctcttaga gactctggaa cgccgcagcc
     2281 ctgatcggcg acttcaatca ccacatcatc gccttcatcg ctgaggaata attcaacgat
     2341 cttgtttcct tcatcgctac gcaggctggc ttcgaaggcg ttatcaagta aattgccaac
     2401 aatggcggca aactcggtgc tatccagtcc tggcggcagt tgcgaaagct ggctaccggg
     2461 gacgatgatc atttttagcc ccagttcgcg ggcgcgctgc actttaccaa aaagcagccc
     2521 cgccacctgg cgatcggcaa acgcctcacg caggctgtca ataagctgtt gctgggcctg
     2581 agactccccc tgcaccatcg ccagcacgcg atcatactct ttcatctgca acagaccatt
     2641 gagcgtcgac atccaattgt gatgctcgtg tcgcaatgta cgaaggctct cgacgtattg
     2701 ttttatttgc gtcagttgcg cattgagggt ggaaatttcg tctttactac gaaagctgat
     2761 aatggccccc agcaaatcat cacctgaacg aatagcttcc cggttggcaa taacgctcag
     2821 accattaaag ttcgccacca catcctgacg tttttcatca atctgttcgg taaagaaatc
     2881 ggcgggcctg accacttcag caatgggttt acccaaccat tgccgtccgg gagagctcag
     2941 ccccagcatc tttcttgcgt tacgattgat ggcggtaatg taaccatgcg gatccaccgc
     3001 aatcagccct tcataaaccg aactaaacag cgcctcttgc tgacggacca cgcgtgcgat
     3061 ttgctttggc tccatgccca tcatttgtcg acggatatgc gcggccagga accacgacag
     3121 caacatcaga atccctaaca gcacgacaaa cacgcctgcc atcggtaata aaaactcagc
     3181 ccgccagcta tcgattttac tcaccaggta gccaatcgac accacgccga tgacttttcc
     3241 atcgtcatca aagattggcg ttttggcgcg catcgccatc cccattgccc ctttcccggt
     3301 aatgaagtag ctctcccctt tctccagcgc gccctgtttg gtgaactgca taggataacc
     3361 aattttctcc ggattaagat ggtaaaggcg gatcgagtgc cggtcgccaa tcaccacata
     3421 atcaaaatcg gtatctcttt gtaatttgtt agcgatggtc gccagccgtt tgtagtcacg
     3481 cgttttcacc gcagagatga cactgtcatt ggaggcaata attttcgcct gattcattgc
     3541 catgtcgcgt acatgaagcg ttaaatagtc ctcaaaactg gccgtaaaat attgcgccag
     3601 ggctgcaatg acaaatattg agaacaccag aatcagcaaa aagatacgca gcggaaatgc
     3661 cagtctttgg aaaaatgcaa actgtttatt ctcgttaagc tgcaacattg ttttccttgc
     3721 gggttgtccc gagcctcttt gacggatgta attaa
//
LOCUS       DAEEKQ010000272         3735 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.277, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000272 DAEEKQ010000000
VERSION     DAEEKQ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3735
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.277"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..182)
                     /locus_tag="KLK57_004112"
     CDS             complement(<1..182)
                     /locus_tag="KLK57_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HBE0894331.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTIIISAVGSI"
     gene            426..683
                     /locus_tag="KLK57_004113"
     CDS             426..683
                     /locus_tag="KLK57_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HBE0894332.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            complement(733..1683)
                     /gene="uspE"
                     /locus_tag="KLK57_004114"
     CDS             complement(733..1683)
                     /gene="uspE"
                     /locus_tag="KLK57_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HBE0894333.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="KLK57_004115"
     CDS             complement(1835..2587)
                     /gene="fnr"
                     /locus_tag="KLK57_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HBE0894334.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="KLK57_004116"
     CDS             complement(2782..3297)
                     /gene="ogt"
                     /locus_tag="KLK57_004116"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HBE0894335.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLRDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3308..>3735)
                     /locus_tag="KLK57_004117"
     CDS             complement(3308..>3735)
                     /locus_tag="KLK57_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HBE0894336.1"
                     /translation="FIAVGLTDILESSGLSGIPAFVGLALLSSFLCMFIASGSAIWSI
                     LAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPFVPLFLGFLQRYKPDAKLGT
                     YYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS"
BASE COUNT         1003 a          886 c          893 g          953 t
ORIGIN      
        1 ataatgctac caacagcact gataattatg gtgtagatta aaaattgcac cgcatggaat
       61 aaaaaacctt ttcttttacg atttccgcgg cgaaaccaaa agctcatcag aatcaatgct
      121 gcgcagctac cgaaaataat gaccagatta agcgcattat ttgtaaacag ttcagcgatc
      181 attgttttat caggctcctc cagataattg tcgtcatgcc ggaaacccct ggcggggcta
      241 ttttaccgcg acaattcatt cagatcatca atagtcaggg aaggaagtag caacattagc
      301 taactaatca atgcgttgat tgtaaatcca gctaagaggt gaggttttca gagcagacaa
      361 cggtgaaatg tcatggtatt gttacgttta ggtaacaaga aatttgtctg cacaaggatt
      421 acatcatgat tatggcgaaa ctgaagtcag cgaaagggaa aaaatttctc tttggtttgt
      481 tggcggtttt cattattgcg gcgtcggttg tgactcgcgc gactatcggc ggcgttatag
      541 aacagtacaa tattccgctg tctgagtgga cgacatcaat gtatgtgatt cagtcatcga
      601 tgatttttgt ttatagcctg gtctttactg tgttgctggc aatcccgttg ggaatttatt
      661 tccttggcgg cgaagagcag taagtaaaaa ataggcccga taactcgggc cttgtcagtt
      721 attgaagagt cgttaatcgt cttcttcgtc atccagttca acgggtgtct gatactggtc
      781 aggtttaatg accaacagat cgcagcgaag atgatcaatc acctgttccg ccgtgttgcc
      841 gaggaatgct gctgaaatac cggtgcgtcc taccgtgccc agaaccacaa tccccgcctg
      901 taagtgctcc gccaaatcag gaataacctc ttccggcaga cctttttcta cgtgcgtcat
      961 gttttcatta atgccgaatt tctgccgcag agctttcatt gccagcaaat gttgcccacg
     1021 aatggcatcg ttataaacgc tcggatcaaa ttccggcagt tcaatcgcga tattaattgg
     1081 cgttactggg taagcgccaa ccagatgaac ttcggtatgg ttgacttgtt ctgccagttc
     1141 gatcgtctct ttgaccagtt tttcattgag cgcattatga tacggctctt cactggcgag
     1201 attcaccgcc accagcgcct tacctccttc cggccacggc tggtctttca ccatccacac
     1261 cgggcttggg catttgcgta acagatgcca gtccgttggc gtaaaaatca ccgcttccag
     1321 acggtcatgt tggtgcgcca tttttagcac caaatcgtgc ccgccgctga tcacttcctg
     1381 aatgatggct tcgaagggac ggttatgcca gaccacttta atttcaatgg gaacgccagc
     1441 attgagataa tattttgcct gctcgtggat ccaggctgta cgctggctga tgacgccctg
     1501 acgcatagcg gtacgttcgt ccggggagag cagggtggtc atttcgtatg agaagtcata
     1561 gatcggcaaa aaggctttaa ttttgccacc aatccgttga tgtaaataaa cagctcgccg
     1621 caatgctggt tggtcgtcct ggttaggatc gataacaacg agcatgttct gatacatagc
     1681 catacagggt ctccttacaa caactgtcaa cgcagtttgt aattaaaaga ttaacccata
     1741 tctggtgaat gaaacagtga tgaaccttct gccagatcaa taaatcagaa aaatttaatg
     1801 atatgacaga aggatagtga gttatgcgga aaaatcaggc aacattacgc gtatgaccag
     1861 caagctgggc cagcgcatcg ttattttcga tggtgatgta tttacctttg actgccagca
     1921 taccgctttt ctggaagcga cctagcagac ggctgatggt ttctaccgtc aggcccagat
     1981 agttaccgat atcgccacga gtcatcgtca ggcggaattc acgaggggag aagccgcgtt
     2041 gggcaaaacg acgggacagg ttgtagatga atgcagccag acgttcctcg gcatttttct
     2101 tcgacaacag caagatcatg tcctgatcgc ctttgatttc accgctcatc agacgcatca
     2161 tctgctgacg cagattcggc attttaccgg acaaatcgtc cagcgtttcg aacgggattt
     2221 cacataccat cgaggtttcc agcgcctgag cgaagctcgg gtgatggccg ctgccgatgg
     2281 cgtcaaatcc caccaggtcg ccagctaaat ggaaaccagt gatttgctcg tcgccttgct
     2341 cagtgatggt ataactttta atcgtaccgg agcggatggc ataaagcgat ttaagttcat
     2401 caccagcctt aaacagcgtc tggcctttct gaataggctt cttccgctca atgatattat
     2461 caagctgatc aagctcatgt tcgttgagtg tgaacgggat gcaaagctgg ctgatgctgc
     2521 aatcctggca atggatagca caaccgccag actgaatgcg ccgtataatt cgcttttccg
     2581 ggatcatagg tctgctcaag ccgtaattga tatttgtcaa ttttaacatc tttttaggga
     2641 gcaagtaagt ctaagcaaac cttaacagca gagaattccg atattagatg taaatatatg
     2701 tctatctatt tgaaaaccct taagttgtta agggtaactt tacataaaag tgtgaacaag
     2761 ctggcacaaa ttgtttaatg tttacagcaa aagataacct tcgtggcgca ataaccactc
     2821 ttttcgctga actccgcctg catatccggt catggtgccg tttcggccaa taacccgatg
     2881 gcaaggtacg acgatgctga tgggattcga tccgtttgcc gcaccaacgg cacgcgccgc
     2941 gccagggcgg cccaattgtt cagccagttg gccgtaatgc attacctgtc cgcaggggat
     3001 agtgcgtaga gttttccaga cttcgcgctg aaatggcgtc ccccctgtgg cagtgggaag
     3061 cgtatcaata atgctaagat taccggcaaa ataatcacga agcttgtcgc ttaaaccgcc
     3121 tgggttggtg gcagaaatgc gctcatagcc ttctttgcga taatggatgt ccagcagctg
     3181 caccatgcgt tcgctgtact cttcccattc aaccgcccgc aggcgaaatt gctcatcgca
     3241 aatcacccac agtggaccca gtggcgtggc aattttttct tcaagtaatc tcagcatccg
     3301 ttctctctta agacaaacgt gggtaaatcc ccggacctat cggcagacca acaagatacc
     3361 acgccagcaa catcagcagc cataccacca aaaagataag cggatagggc aagactaacg
     3421 aatagtaagt acccagtttc gcgtctggtt tgtagcgttg caggaatcca agaaacagtg
     3481 gaacaaaagg agataccggc gctaaaggca atacggatga gtcggcaata cgaaagagga
     3541 tttgcgcaaa tgccgggtga aagccaagta gcataaacat tggtacgaaa atgggggcca
     3601 gaatcgacca gattgcggaa ccgctggcaa taaacatgca taagaaagag gaaagcaacg
     3661 ccagaccgac aaacgccggg atgccgctaa gccctgaact ttccagtata tcggtcagcc
     3721 ccacggcgat gaatt
//
LOCUS       DAEEKQ010000273         3699 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.278, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000273 DAEEKQ010000000
VERSION     DAEEKQ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3699)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3699)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3699
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.278"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            2..394
                     /locus_tag="KLK57_004118"
     CDS             2..394
                     /locus_tag="KLK57_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBE0894337.1"
                     /translation="MLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVEGLAIE
                     RGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDADYDVRL
                     VQDCCYDPDRDAHEALLRSGFGGRVQVV"
     gene            532..1416
                     /locus_tag="KLK57_004119"
     CDS             532..1416
                     /locus_tag="KLK57_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBE0894338.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(1448..2647)
                     /gene="tet(A)"
                     /locus_tag="KLK57_004120"
     CDS             complement(1448..2647)
                     /gene="tet(A)"
                     /locus_tag="KLK57_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HBE0894339.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            2753..3403
                     /gene="tetR(A)"
                     /locus_tag="KLK57_004121"
     CDS             2753..3403
                     /gene="tetR(A)"
                     /locus_tag="KLK57_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HBE0894340.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLVVRNVEGPRKGDD"
     gene            complement(3435..3677)
                     /locus_tag="KLK57_004122"
     CDS             complement(3435..3677)
                     /locus_tag="KLK57_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase"
                     /protein_id="HBE0894341.1"
                     /translation="MKTAALDLARERQAHEAGARTRATAHERTPQQERQKAAREAERG
                     REAWTLGQGMKKPVAGCYGRLTRWKGGGDVVYMALL"
BASE COUNT          686 a         1144 c         1162 g          707 t
ORIGIN      
        1 cgtgctgttc cccaactttt cgctcggcga agagtatgaa catgcgccgc cggcgacgaa
       61 tcgccaaatc tcaccgtatc tcccgagcgg acgatttcgc accggtctgc ccgtcgaagg
      121 gcttgcgatc gaacggggcg accttttcta tgcatgtccg cgagccagcg tcttttatgg
      181 cacggcgctc gacgccgacc ttcggacgcg cggcgtaagc acgcttgtca tggccgggat
      241 aagcaccacc ggcgttgttc tttcaagcgt cgcctgggct agtgatgcgg actacgacgt
      301 gcgtttggtc caggactgct gctacgaccc ggatcgggat gcccacgaag ctctgttgcg
      361 ttccgggttc ggcggacgtg tacaggtcgt gtaattttcg ggcctcgcat gatcgcggcc
      421 atagccgcag gtggattggt gcgaggcagg gccagtccaa gtcagggtgc ggtcatcgcg
      481 gcgcctgcaa ctctcaaaaa cccatcgccg tagcggaggt cttgtaatcg catgtcatta
      541 cgcacgcccg atttattgtt cacagcgata gcacctgcca tttggggcag cacctacatt
      601 gtcaccaccc aatacctgcc gaacttctca ccgatgacgg tcgcgatgct gcgggcgttg
      661 ccggcgggtt tattgctcgt gatgatcgtc cgacagattc caacgggaat ctggtggatg
      721 cgcatcttca tcctcggcgc acttaatatt tcgctattct ggagcttgtt gtttatttcg
      781 gtctaccgcc tgccgggcgg ggtcgcggcg acggtaggcg ctgtgcagcc gctgatggtc
      841 gtgttcatct ctgccgctct gctaggtagc ccgatacgat tgatggcggt cctgggggct
      901 atttgcggaa ctgcgggcgt ggcgctgttg gtgttgacac caaacgcagc gctagatcct
      961 gtcggcgtcg cagcgggcct ggcgggggcg gtttccatgg cgttcggaac cgtgctgacc
     1021 cgcaagtggc aacctcccgt gcctctgctc acctttaccg cctggcaact ggcggccgga
     1081 ggacttctgc tcgttccagt agctttagtg tttgatccgc caatcccgat gcctacagga
     1141 accaatgttc tcggcctggc gtggctcggc ctgatcggag cgggtttaac ctacttcctt
     1201 tggttccggg ggatctcgcg actcgaacct acagttgttt ccttactggg ctttctcagc
     1261 ccggggaccg ccgtgttgct aggatggttg ttcttggatc agacgctgag tgcgcttcaa
     1321 atcatcggcg tcctgctcgt gatcgggagt atctggctgg gccaacgttc caaccgcact
     1381 cctagggcgc gtatagcttg ccggaagtcg ccttgacccg catggcatag gcctatcgtt
     1441 tccacgatca gcgatcggct cgttgccctg cgccgctcca aagcccgcga cgcagcgccg
     1501 gcaggcagag caagtagagg gcagcgcctg caatccatgc ccacccgttc cacgttgtta
     1561 tagaagccgc atagatcgcc gtgaagagga ggggtccgac gatcgaggtc aggctggtga
     1621 gcgccgccag tgagccttgc agctgcccct gacgttcctc atccacctgc ctggacaaca
     1681 ttgcttgcag cgccggcatt ccgatgccac ccgaagcaag caggaccatg atcgggaacg
     1741 ccatccatcc ccgtgtcgcg aaggcaagca ggatgtagcc tgtgccgtcg gcaatcattc
     1801 cgagcatgag tgcccgcctt tcgccgagcc gggcggctac agggccggtg atcattgcct
     1861 gggcgagtga atgcagaatg ccaaatgcgg caagcgaaat gccgatcgtg gtcgcgtccc
     1921 agtgaaagcg atcctcgccg aaaatgaccc aaagcgcggc cggcacctgt ccgacaagtt
     1981 gcatgatgaa gaagaccgcc atcagggcgg cgacgacggt catgccccgg gcccaccgga
     2041 acgaagcgag cgggttgaga gcctcccggc gtaacggccg gcgttcgcct ttgtgcgact
     2101 ccggcaaaag gaaacagccc gtcaggaaat tgaggccgtt caaggctgcc gcggcgaaga
     2161 acggagcgtg gggggagaaa ccgcccatca gcccaccgag cacaggtccc gcgaccatcc
     2221 cgaacccgaa acaggcgctc atgaagccga agtgccgcgc gcgctcatcg ccatcagtga
     2281 tatcggcaat ataagcgccg gctaccgccc cagtcgcccc ggtgatgccg gccacgatcc
     2341 gcccgatata gagaacccaa aggaaaggcg ccgtcgccat gatggcgtag tcgacagcag
     2401 cgccggccag cgagacgagc aagaccggcc gccgcccgaa acgatccgac agcgcgccca
     2461 gcacaggtgc gcaggcaaat tgcatcaacg catacagcgc cagcagaatg ccatagtggg
     2521 cggtgacgtc gttcgagtga accagatcgc gcaggaggcc cggcagcacc ggcataatca
     2581 ggccgatgcc gacagcgtcg agcgcgacag tgctcagaat tacgatcagg ggtctgttgg
     2641 gtttcacgtc tggcctccgg accagcctcc gctggtccga ttgaacgcgc ggattcttta
     2701 tcactgataa gttggtggac atattatgtt tatcagtgat aaagtgtcaa gcatgacaaa
     2761 gttgcagccg aatacagtga tccgtgccgc cctggacctg ttgaacgagg tcggcgtaga
     2821 cggtctgacg acacgcaaac tggcggaacg gttgggggtt cagcagccgg cgctttactg
     2881 gcacttcagg aacaagcggg cgctgctcga cgcactggcc gaagccatgc tggcggagaa
     2941 tcatacgcat tcggtgccga gagccgacga cgactggcgc tcatttctga tcgggaatgc
     3001 ccgcagcttc aggcaggcgc tgctcgccta ccgcgatggc gcgcgcatcc atgccggcac
     3061 gcgaccgggc gcaccgcaga tggaaacggc cgacgcgcag cttcgcttcc tctgcgaggc
     3121 gggtttttcg gccggggacg ccgtcaatgc gctgatgaca atcagctact tcactgttgg
     3181 ggccgtgctt gaggagcagg ccggcgacag cgatgccggc gagcgcggcg gcaccgttga
     3241 acaggctccg ctctcgccgc tgttgcgggc cgcgatagac gccttcgacg aagccggtcc
     3301 ggacgcagcg ttcgagcagg gactcgcggt gattgtcgat ggattggcga aaaggaggct
     3361 cgttgtcagg aacgttgaag gaccgagaaa gggtgacgat tgatcaggac cgctgccgga
     3421 gcgcaaccca ctcactacag cagagccatg tagacaacat cccctccccc tttccaccgc
     3481 gtcagacgcc cgtagcagcc cgctacgggc tttttcatgc cctgccctag cgtccaagcc
     3541 tcacggccgc gctcggcctc tctggcggcc ttctggcgct cctgctgcgg cgtccgctcg
     3601 tgggccgtgg cgcgggtccg cgcgccggcc tcgtgcgcct ggcgctcgcg ggcgaggtcc
     3661 agggcggccg tcttcacgtt ctgccttgcg cagatgaga
//
LOCUS       DAEEKQ010000274         3684 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.279, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000274 DAEEKQ010000000
VERSION     DAEEKQ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.279"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..401)
                     /locus_tag="KLK57_004123"
     CDS             complement(<1..401)
                     /locus_tag="KLK57_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0894342.1"
                     /translation="MKLLVTYIRWSTKEQDSGDSLRRQTNLIDAFYSKHKNDYYLLPA
                     HRYVDKGKSGFHQQHKNQGSDFRRMFENVMSGAIPEGSLIVVENFDRFSRADIDTAID
                     DVRQILRKGVSILTLGDGELYDKSALTDPVK"
     gene            453..1058
                     /gene="wrbA"
                     /locus_tag="KLK57_004124"
     CDS             453..1058
                     /gene="wrbA"
                     /locus_tag="KLK57_004124"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HBE0894343.1"
                     /translation="MSVKARILVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPE
                     TMPPQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWA
                     SGALYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGG
                     TPYGATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            1079..1306
                     /locus_tag="KLK57_004125"
     CDS             1079..1306
                     /locus_tag="KLK57_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HBE0894344.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKADKDSLFWGEQTIERKNV"
     gene            complement(1344..2585)
                     /gene="agp"
                     /locus_tag="KLK57_004126"
     CDS             complement(1344..2585)
                     /gene="agp"
                     /locus_tag="KLK57_004126"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HBE0894345.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(2879..3684)
                     /locus_tag="KLK57_004127"
                     /pseudo
     CDS             complement(2879..3684)
                     /locus_tag="KLK57_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000535347.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          979 a          887 c          818 g         1000 t
ORIGIN      
        1 aacttcacag ggtctgttaa ggctgacttg tcgtagagtt caccatcacc cagagtcagt
       61 atagagaccc ccttcctcag tatctgcctg acatcatcta tagctgtgtc gatatcagcc
      121 ctactgaagc ggtcaaagtt ctctacaaca atcaaggaac cttctggaat agccccagac
      181 ataacatttt caaacatacg tctgaagtca gaaccttgat tcttatgttg ctggtgaaaa
      241 ccagatttac ccttatcaac atatctgtga gcaggaagaa ggtaataatc gtttttgtgc
      301 ttagagtaga aagcatcaat gaggtttgtt tgccgtctta aggagtctcc tgagtcttgc
      361 tctttagtac tccacctgat gtatgtcact agcagcttca tatcttcgga ctatgcttat
      421 ctaagtggtt cattgaacag gagaacaatg atatgagtgt taaggcaaga atactagtgc
      481 tttattattc catgtacgga catattgaaa cgatggcacg cgcagtcgct gagggtgcaa
      541 gcaaagtcga tggcgcagaa gttgtcgtta agcgtgtacc ggaaaccatg ccgccgcaat
      601 tatttgaaaa agcaggcggt aaaacgcaaa ctgcaccggt tgcaaccccg caagaactgg
      661 ccgattacga cgccattatt tttggtacac ctacccgctt tggcaacatg tccggtcaaa
      721 tgcgtacctt cctcgaccag acgggcggcc tgtgggcttc cggcgcacta tacggaaaac
      781 tggcgagcgt ctttagttcc accggtactg gcggcggtca ggaacaaact attacttcaa
      841 cctggacgac ccttgcgcat cacggcatgg tgattgtccc cattggctac gcagcgcagg
      901 aattatttga cgtttcacag gttcgcggcg gtacgccgta cggcgcaacc accatcgcag
      961 gcggtgacgg ctcacgccag ccaagccagg aagaactgtc tattgctcgt tatcaagggg
     1021 aatatgtcgc aggtctggca gttaaactta acggctaatc ttcaacagga ggatacgcat
     1081 gccaactcaa gaagcgaaag ctcatcacgt cggtgaatgg gcatctctgc gcaatacgtc
     1141 gccggaaata gccgaggcca ttttcgaagt cgccgggtac gacgaaaaaa tggcagaaaa
     1201 gatttgggaa gaaggtagcg atgaagtgtt agtcaaagcc tttgctaagg ccgataaaga
     1261 ttcgcttttt tggggcgaac agaccatcga acgtaaaaac gtttaaccag cgactccccc
     1321 gcttctcgcg ggggagtttt ctgttatttc accgcttcat tcaacacgct atcaaattta
     1381 tccatcgggc agaaaccgtt ggcatctatc gggcaaccgc ttaattccag cgtcacacgc
     1441 tgagcaggtg cctgcagggt taacgcatcg gcattacgta actgttccgc actctgatac
     1501 acatattcaa ttttcatcaa atcgcgattg gctttgctgt catgccaacg ctggaaaacg
     1561 attttgccgc caatcggcgt gcgttcgttc tggtcatgca actgatacgg tttgaaatcc
     1621 agcgccgtta acagagaggc aatgttggag tcgtgcccaa ccaacactgt aattttcggt
     1681 gcgctggtgc gatcggtgac cagagctttg tcgatataac tgaccagcgg tttcgcaaca
     1741 ttgcgcgcca cttccggtga ggtaaacagg ctgtcctggt agccgttttt aagcttcgac
     1801 aacaccttcc actgctggtc agatttgatt tctccccagg ccacctgatc catcggaaaa
     1861 ccttcgtaat attgcaaagt aaacgcatct accagcgagt tgccgacttt cagcggcccg
     1921 gaaacacctg gttcttgttg atacttggcg ctaaaggtat ttttgccatc caccagcgaa
     1981 cactgttgtt tctctttaca ggcaggggaa tctttatagt taacgatttt ttccagtagc
     2041 tggtagctgt cggtaagctg gagtttgctg agctctttct ccattgccgc caccgcctgt
     2101 tcactgaatg cggcggaatc atcggtgatc accgggttaa aggttgggtc catagtgccc
     2161 attttttcct ggtgatgcac aggaatatca caccccggga atgcgccggt aataaagaac
     2221 tgtgcggtcg caacggtacg ttgcagacta ttggcatagg cataaacggt gtccggcggc
     2281 gggcattccc ccgatttcac catcccctgc tctgccagcc attcacgcat gtaatggccc
     2341 atatacactt cgagcacgcc acctttggtg gtgagttgcc caccggggac gtcccattct
     2401 ggccatttat tcggcgtcga ctgctccagc acactgccat tgttcgccag cggcgcacgt
     2461 aagttatggc ggctcatcat gagcacttgc tgtagctgat agccttccgg tacggtttgt
     2521 gcctgagcat ttgaagcgag taaaactatc cctgccacag ttgcggcgat tagcgttttg
     2581 ttcattcctg gcacctcttt tgttatcaat cattaaagag tgtgacagaa atatgacttt
     2641 tttccgggaa caggttcgca aaatggcttg ttgaacgcct gtttttctat atacatcttc
     2701 aaaaacaggc aaggtttgac gcaaactatg cctgattagc ccctgcctcg cgtacgccgc
     2761 tgataattgc acctgtggat aaacagaata tcagcggcgt tactaataca gaggcaatag
     2821 taccaataat aagagaagtg aggctaataa caatggtttt tagcacatgc ctttgtcatt
     2881 aaattgcata acgagttaaa aaatcaattt gcgcattcct ttccttattg tttaactcgt
     2941 aagcctgagc aaaaagttca ggagggaggt aaacgtcata atgattgcca gtgtttatta
     3001 aatacccata ctcaaggtcg tcatctgttg aaaaggattc attccctaag gagattgatg
     3061 ccacattgct gtaaacaata aataatgagc aaatgctatc tgaaagagca gtgaggaaat
     3121 ttttcacctc attaaccttc tgctctgggt cggtgtctaa aacaaggcgt gccttgttat
     3181 cattaaatat aaattcgggt aaatgcagta tgcatttttt gtcattcaag caactatcaa
     3241 taaattcttt tttaaaagac aaaaaactcc atgataaaaa accaccctgc ccctgaattt
     3301 tgttgtcaga ttgtagatta acgccaaata acgtctttac atcttccctg agattataga
     3361 atgccgtatc aacaagagcc tcgatcgttt gtttatcaac aattttaata aactcttcgt
     3421 atacttgctt ttgcgtctta tggccgccca gcgcccatga atactcatgt tcttttaaaa
     3481 ccagtatcta aatcagatat tcccggcgta tttatctgtt ctggctggat actcatattt
     3541 tcatcatcta aacaatactc agaaaaaata tcatcacacg cagtaatgtt ttcaatatat
     3601 tccataaagc cataccaata ataaccttta aaatctgtga tagactctga acctctgccc
     3661 ggcaaggaca ctctaacaat atca
//
LOCUS       DAEEKQ010000275         3627 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.280, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000275 DAEEKQ010000000
VERSION     DAEEKQ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.280"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(89..652)
                     /locus_tag="KLK57_004128"
     CDS             complement(89..652)
                     /locus_tag="KLK57_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HBE0894346.1"
                     /translation="MNRTSIEKLPSLTHNHLPVMTTELLADLYGTERQRLTNNFNRNK
                     ERFIEGKHFFLIEGDALRELKNENSLRVSVKIARNVRSLILWTERGAARHAKMLETDQ
                     AWEVFEKLEDCYFNQKQPPAAQNTSIENDGCALLSHFDKHGQVEFTEKVPADAMVCTL
                     ERFKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(756..974)
                     /locus_tag="KLK57_004129"
     CDS             complement(756..974)
                     /locus_tag="KLK57_004129"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894347.1"
                     /translation="MGELKSSPVGFPVVPTLCVSPPDDWNLTVLTVSGLQFTFPRGAQ
                     NTSKAVSTPDSHVVFLCPEFLVYDRVCG"
     gene            complement(1133..1519)
                     /locus_tag="KLK57_004130"
     CDS             complement(1133..1519)
                     /locus_tag="KLK57_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBE0894348.1"
                     /translation="MARDIQQVMERWGAWAANTHEDVSWASIAAGFKGLIPSKVKSRP
                     QCSDDDAMIICGCMARLNKKNQDLHDLLVDYYVGGMTFMSLARKHGCSDTCIGKRLQK
                     AEGVIDGMLMMLDIRLEMDRYVERIM"
     gene            complement(1509..1880)
                     /locus_tag="KLK57_004131"
     CDS             complement(1509..1880)
                     /locus_tag="KLK57_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBE0894349.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKVGERYRRDVTLIVRQQ
                     RLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVRGQ
                     LVPGGRLGVKIYEITGDNDGA"
     gene            complement(1880..2137)
                     /locus_tag="KLK57_004132"
     CDS             complement(1880..2137)
                     /locus_tag="KLK57_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894350.1"
                     /translation="MNNQTMTFTSEQLRKHAQEMLRQAEQLEKTGVTKDAIRRDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETKVGKFEKMVQEVLR"
     gene            complement(2134..3525)
                     /locus_tag="KLK57_004133"
     CDS             complement(2134..3525)
                     /locus_tag="KLK57_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HBE0894351.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADHEVMDILITLPADIFSVRA
                     YRDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWARFSQGVGRMTG
                     RNIWMVDQANLTIDEICATTKHHLIKHPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQATGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESAQVLSTPMRQPQPRRYS
                     NTRTDSSKMERFF"
     gene            complement(3522..>3627)
                     /locus_tag="KLK57_004134"
     CDS             complement(3522..>3627)
                     /locus_tag="KLK57_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBE0894352.1"
                     /translation="FISPRIADRITDGGRNWLSFNWPSYRSCIRGVAA"
BASE COUNT          850 a         1013 c          811 g          953 t
ORIGIN      
        1 catcggcggg tttaagctgt gtggcgtagt aaccactctt aacaggatat tcaacttttt
       61 acgatcgtaa agttttcgga aaaaattttt aaaaccgcat caaccgctcc accagttgct
      121 ctttacgggc aacgatccac ccgtgttgct ccagataaaa tttaaaccgt tccagagtac
      181 ataccatcgc atcggcgggt accttttccg tgaactcaac ctgaccgtgt ttatcgaagt
      241 ggctcagtaa tgcgcatcca tcattttcga tagatgtgtt ttgtgccgct ggtggctgtt
      301 tctggttgaa ataacagtct tccagttttt cgaacacttc ccacgcctga tcggtttcga
      361 gcattttggc gtgacgggct gcgccacgtt ctgtccagag gatgagggag cgaacgttac
      421 gggcaatttt cacagaaact cttaaagagt tttcgttctt caactctcgt aacgcatcac
      481 cttctatcag gaaaaaatgt ttaccttcta taaaccgttc tttgtttcga ttgaaattgt
      541 ttgtcaaacg ctggcgctct gttccgtaca aatcagccag cagctctgtt gtcatgacag
      601 gaagatggtt atgcgtaagt gacgggagtt tttcaataga cgtgcgattc atagacatgt
      661 cctttcgaat attttgattt accccttttg agagggtgac cgggcgctca aaaccgtcga
      721 aagtcggcgg gcgtatttcc attgctggta ttgtattagc cgcacacccg gtcataaacc
      781 aagaattctg gacacaaaaa aaccacatgg ctatcgggtg tggataccgc tttcgaggtg
      841 ttttgagcac cgcggggaaa tgtaaactgc aaacctgaaa ctgtcaacac cgtcaggttc
      901 caatcatcgg gtggtgagac gcacagggtt ggaactaccg ggaaaccgac cggcgagctt
      961 ttcagctccc ccatgcgccc caccataatt cagatgtgcg cgtgcatacg acaataaaaa
     1021 acacgctcgc ggcgtgtgtc tgtcgcggtc tctatccggg gttccaatcc cgacgccaga
     1081 ttttgctggc gcgtgaggaa tatagccccg gacaatgtgt ctggtcaagc acctacataa
     1141 ttcgttctac gtatctgtcc atctccagcc ggatatcaag catcatcaac atgccatcaa
     1201 taaccccttc cgctttctgc aggcgcttgc caatacaggt atccgaacac ccatgtttcc
     1261 gtgccagact cataaaagtc attccaccta cgtaataatc caccagcaaa tcgtgcaaat
     1321 cctgattttt cttgttcaac cgggccatac agccacaaat tatcattgca tcatcatcag
     1381 aacactgagg gcgtgatttc actttcgacg gaattaaccc tttaaaacca gcagcgatcg
     1441 acgcccagga tacatcttca tgagtgtttg cagcccaagc cccccaccgt tccataacct
     1501 gctgaatatc acgcgccatc gttatcacct gtgatttcat aaatcttcac gcccagtcgc
     1561 ccaccaggaa cgagctgacc gcgcacaata ttgatttcat caaactgctc gtcgtctatg
     1621 agtagtccgg catgcgtcag cgcatccagt ggtgctttca ggatattgtc caggtcacga
     1681 cggcgcttat ccggtggctc tgcaataatc tttatcgcca gccttccgga caggtttaat
     1741 ttcagccgct gctggcgaac aattagcgtc acatcacggc gataacgctc accaactttt
     1801 gatacaaaat atgtgctgcc acgacgtcgc cagtaagtat tcaccgtcgg cggataaggt
     1861 aaaataaact catggcgcat cagcgcagca cctcctgcac cattttttca aactttccaa
     1921 ctttggtttc cagctctgcc acacaatcaa cgagctcatc caccgctttt tgtgcgcgat
     1981 gtttcgcctg catcagttcc ctgagcgctg gcaccatatc ccgacgaatg gcatcttttg
     2041 ttacacctgt tttttccagt tgttccgcct gtcgcaacat ttcctgcgcg tgtttacgca
     2101 attgttcaga ggtaaaagtc attgtctggt tgttcaaaag aaacgctcca tcttactgct
     2161 gtcggtacgt gtattgctgt atctgcgcgg ctggggctgc cgcattgggg tggaaagaac
     2221 ctgtgcgctt tcctggtcca caggcagaaa atgtccgtta taaaaacgcc ggtaaatcgt
     2281 ccccagagaa ccgttacgtt gtttcgtgat attaatttct gctatgcctg tagcctgtgt
     2341 atccgggttg tacacttcat ccctgtaaag catcagaatg atgtctgcat ccgcctctat
     2401 ttctccggaa tttttcaggt ctgagttcat gggacgttta ttgggtctgg actccacacc
     2461 gcgggagagc tggctcagcg caatcaacgg aaaaccaccg gattttgcca ggcctttaag
     2521 cccctttgag atttcaccca cggcaaggtc atgacgcccc gtggttcggg tttttatcag
     2581 cccgagataa tcaaccacca ccagcgccgt ttccggatgt ttaatcagat ggtgtttcgt
     2641 tgttgcgcat atctcatcaa tggtcaggtt cgcctggtcc accatccaga tattgcgccc
     2701 ggtcatccgc cccacccctt gtgagaaacg cgcccagtct tcatcttcaa agtgagccac
     2761 agatttcagg cgtgatactg gcatccctcc agccgcagac accatacgtt caccaatctg
     2821 gatgttcgcc atctccatgg tgaacagaag cacaccatgc ccctgctcag tcaccttgtc
     2881 gatgatgtcc agcgcaagtt cggttttccc catcgaagga cgggcggcaa tgaataccag
     2941 gtctccgggc tccataccgc ctgtttttgc gtccagttca tcaataccgg tcatcaacgt
     3001 cctggatttc tccagcccct gattccggca ttcaacacgc tcaaccactt ccggaagcac
     3061 atcatcaata tgtaccggct gaatgacgcc ctttcctgtc gacagtgtga ccatcatgtt
     3121 ctgcgcatcc ttcagggcat cttcagctgc ttcacaggta tgcgcatcac gtaatttctg
     3181 cagcgcctca ttcagtgttt tttctgcatc gcgcagtgcg gcattgcgcc gcaacgctgc
     3241 aacatagtgc tccagtgaag acttcaccca ggttttacgc ccggtatcag taatcaccgg
     3301 ggcaagttcc ggcatctcat tacacaacag cacggggtca atcactcctg aaacacgggc
     3361 ctgtctgcag atgcctgtgt agatatcccg atacgctcgt acagaaaaaa tgtccgccgg
     3421 tagtgtgatc agaatatcca tcacttcatg atctgcccca cgcagaaaga atgcgccaat
     3481 gacagcgcct tccaggtcat cattacgcca gactggtgtt gtcatgcagc cacacctctg
     3541 atacaagaac ggtagctggg ccagttgaac gacaaccagt tgcgcccccc gtctgtgatc
     3601 ctgtcggcaa tgcgggggct gatgaac
//
LOCUS       DAEEKQ010000276         3622 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.281, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000276 DAEEKQ010000000
VERSION     DAEEKQ010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.281"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..2218
                     /locus_tag="KLK57_004135"
     CDS             <1..2218
                     /locus_tag="KLK57_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000433376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HBE0894353.1"
                     /translation="DHFLEAVISISGLVGHPFNANFLHDWLKDYSLANRDSFWTTELK
                     YKYSEDSAFRHLIDWAWARTDKSFVSDESIELVATSLCWFLTSSNRELRDCSTKALVS
                     LLEPRIPVLRKIIDKFYGVNDPYVWERIFAVALGCTLRTDNIKELKYLAETVYQKVFC
                     SKYVYPNILLRDYAREIIEFANHLGLELESIELSKTRPPYNSIWPDKIPSKEELESLY
                     DKEPYRELWSSIMEDGDFSRYTIGTNYNHSDWSGCKFNETPVDRKQVFKTFKCKLTDQ
                     QKDLYDATDPFIYDDKCEGIKFGRVVGRKAQEEIKASKKLFKNSLSYDLLSEFENEIE
                     PYLDHNNNLLETDKHFDLRLAQQFIFNRVIELGWDPEKHGNFDQQIGTGRGRREAFQE
                     RIGKKYQWIAYYEYMARLADNFTRFEGYGDERKENPYQGPWEPYVRDIDPTILLKETG
                     TKKISNKEMWWLNDEVFDWTCSNEDWVKSSTTITNSYAFIEVKDDNGDEWIVLESYPS
                     WKEPKIIGNDDWGHPRKEVWYQIRSYIVKVEEFENFRCWAIAQDFMGRWMPECTDRYQ
                     LFNREYYWSEAFKSFKSDYYSGSDWTSVTDRESGAKIADVSVTSINYLWEEEFDKSKI
                     ETLNFLKPSNLIFEKMGLKSGEVEGSFNDENGTMVCFAAEAVYASKPHLLVKKEPFLT
                     MLRDNGFEIVWTLLGEKGVIGGSLISSHHYGRQEFSGAFYYEDSQLTGSHKTSFTR"
     gene            3253..3411
                     /locus_tag="KLK57_004136"
     CDS             3253..3411
                     /locus_tag="KLK57_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001570982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894354.1"
                     /translation="MKNIAAIKRNNRKIHARKFLSTPEGKAWLERKQRENEERKLLSE
                     LKWLREDF"
BASE COUNT         1163 a          564 c          807 g         1088 t
ORIGIN      
        1 tgatcatttc ctcgaggcag tgatctctat ttcaggttta gttggccatc cctttaatgc
       61 taatttcttg catgattggc taaaagatta ttctttggca aatcgagatt cgttttggac
      121 tacagaactt aaatataaat atagtgaaga ctcagcattt aggcatctaa tcgattgggc
      181 atgggccaga acagataaaa gctttgtttc ggatgagtca atcgagctag ttgcaactag
      241 tttatgctgg tttttaactt ctagtaaccg agaacttcga gattgctcaa ctaaggcttt
      301 agtgagttta ctcgagccaa gaattcctgt attgagaaaa ataattgata agttttatgg
      361 tgtaaatgat ccttacgttt gggaaagaat atttgcagtt gcattaggct gtacattgcg
      421 aactgataat attaaagaac taaaatattt agccgaaact gtttaccaaa aggtattttg
      481 ttctaagtat gtgtatccaa atatattact tagagattat gctagagaga ttattgaatt
      541 tgctaatcat cttggattgg aacttgaaag cattgaatta tccaagacta gaccacccta
      601 caacagcatt tggcctgaca agattccttc aaaagaggaa ctagagtccc tttatgataa
      661 agaaccttat cgggaactct ggagctctat tatggaagat ggtgactttt cacgatatac
      721 tattggaaca aattataatc attctgattg gtctggttgc aagtttaatg aaacccctgt
      781 tgaccgtaag caagttttta aaactttcaa atgtaaacta actgatcaac aaaaagactt
      841 gtatgatgcc acagatcctt tcatttatga tgataaatgc gaaggaatta aatttggtcg
      901 tgtggtcggt agaaaagcac aggaagaaat aaaggcgagc aagaaattat ttaagaattc
      961 attgtcatac gatctgttaa gtgagtttga aaatgaaata gagccatacc tggatcataa
     1021 taataatctg ctggaaactg ataaacactt tgatcttcga ctagctcaac aatttatatt
     1081 caatcgtgtt atagagcttg gttgggatcc ggagaagcat ggtaattttg accaacaaat
     1141 aggaactgga cgtggacgta gagaggcatt ccaagaacgg attggtaaaa aataccaatg
     1201 gattgcttat tatgaataca tggcaaggct agccgataat tttactcgtt ttgaaggtta
     1261 tggtgacgaa cgaaaggaaa atccatacca agggccatgg gagccttacg taagagatat
     1321 agatcccact atcttactta aagaaactgg aacgaaaaaa ataagcaata aagaaatgtg
     1381 gtggcttaat gatgaagtgt ttgattggac ttgctctaat gaagactggg ttaaaagttc
     1441 tactactata actaattcat atgcttttat tgaagttaaa gatgataatg gtgatgaatg
     1501 gatagtatta gaaagttatc catcatggaa agaaccaaaa attattggaa acgatgattg
     1561 ggggcaccca cgaaaagagg tttggtatca gatcagaagt tatatcgtta aagttgaaga
     1621 atttgaaaat tttagatgtt gggcaatagc tcaagacttt atgggcaggt ggatgccgga
     1681 atgtactgat agataccaat tatttaatag ggagtactat tggtccgaag catttaagtc
     1741 ttttaaatca gattattata gtggatctga ctggacttcg gtaacagacc gggagtctgg
     1801 agctaagata gctgatgtta gtgtcacttc gattaattat ttgtgggaag aggagttcga
     1861 caaatcaaaa atagaaactt tgaatttttt gaagcctagt aacttaatct ttgaaaagat
     1921 gggattaaaa agtggggaag tagagggtag cttcaatgat gaaaatggaa ctatggtttg
     1981 ctttgcagct gaagctgtat atgcttcaaa gccgcatcta cttgttaaaa aagaaccatt
     2041 tttaacaatg ttaagggaca atggttttga aatcgtttgg acattattag gtgaaaaggg
     2101 cgttataggg ggctcactca tatcaagtca tcattatggt cgacaggagt ttagtggagc
     2161 attttattat gaagacagtc agctaacagg aagtcataaa actagcttta cgagataaaa
     2221 atgaatctca gagctgaata tataagtagt attagaaacc gggttatact taagaaatca
     2281 atcttaagtg tggcagtcga atggtagcta atatgctagc ggcgctaatg cctgtttgtt
     2341 gctcataaca ggcattcact ttagttatgg cagaaaagta tacatgctgg gttgggaaag
     2401 tgtgaaagaa aggaagattg ctgcgccgtt tgtcgtcacg tttatcttca ttggctatgc
     2461 aagtcgtaat aaaaggtggg acaaaactga gacacataaa gcctcgcaat ggcttgcaag
     2521 gctttacatg ttttgatgta gtgggacgtg tgagcgcagt gttgatgggg taatgctttg
     2581 aattagaagc ggattcttat aattcgtaat gcgaaggtcg taggttcgac tcctattatc
     2641 ggcaccatta agaaaattaa taacttagct taacttctct aagaaattgt atcttctgtt
     2701 atcacttaca aacgcctata cgttgttatg ggctttggta gtgtaacacg tatgggcgag
     2761 tagttcccag gctcaacctc gtaacaagtt cctttgaagc tggaagacgt gccaccgtct
     2821 ctaaaccttc taccaaaact cttagcaaga tgttccacac ctggcgcggt gtccaatgat
     2881 gctacacgtc gtgagacgtt gcagaggata aacaggctaa atgatgggaa gagttttaag
     2941 tgcaatagtg gattaaagcc cacctcttca aaacctaact tgatcgggaa agaatcaagc
     3001 tctagcacgg tttcgtccac cggagtacaa cgggtgcctg ataagagaag aactgctaag
     3061 acttcatagc ttttgtgaaa aagccctttt tagtggatac gcctacggcg cttatttttt
     3121 ggtataacca tcaaaggcgc tttatggctc ctattgtctt gaaaatgtat ctttacttgt
     3181 attgtgtttt atgcgtagtc tccctgtgta gtatgtaact tgttaatttt catatgaata
     3241 cagaggcaca cgatgaaaaa cattgcagca atcaaacgta ataaccgcaa gattcacgct
     3301 cgtaagttcc tgtctacgcc agaaggaaaa gcctggctag agcgtaaaca gagagagaac
     3361 gaagaaagaa aactccttag tgagttgaaa tggcttagag aggatttctg aaaatctcca
     3421 gaacgcaaca ggacgcattc taacgctgtt ttcattactc ggtataggga taggtgatgt
     3481 gtgtttaatg cgtctgtgcg cttgttttgt gccttatttt gcattttcat aatgcggaag
     3541 agaacacgga aaaccttaaa attaattatt ggggtattta aggctatttt caggtgattt
     3601 aagggtatta aacttgattt aa
//
LOCUS       DAEEKQ010000277         3581 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.282, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000277 DAEEKQ010000000
VERSION     DAEEKQ010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.282"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(42..1406)
                     /locus_tag="KLK57_004137"
                     /pseudo
     CDS             complement(42..1406)
                     /locus_tag="KLK57_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(1471..1590)
                     /locus_tag="KLK57_004138"
     CDS             complement(1471..1590)
                     /locus_tag="KLK57_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HBE0894355.1"
                     /translation="MPLSYVEHHHFIMMSLMSGEHNTLALSLQDEPPGLVPAG"
     gene            complement(1745..1963)
                     /locus_tag="KLK57_004139"
     CDS             complement(1745..1963)
                     /locus_tag="KLK57_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0894356.1"
                     /translation="MKKLLRTLSAVDARVVCKPHRLGRSMWHLVVLLEELCKRGINFR
                     ALAQSIFAQQWGDECCKSKRICDLKVIV"
     gene            complement(2317..3063)
                     /locus_tag="KLK57_004140"
                     /pseudo
     CDS             complement(2317..3063)
                     /locus_tag="KLK57_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3148..3581)
                     /locus_tag="KLK57_004141"
                     /pseudo
     CDS             complement(3148..3581)
                     /locus_tag="KLK57_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311544.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5507 domain-containing protein"
BASE COUNT          989 a          805 c          645 g         1142 t
ORIGIN      
        1 ataagtgata ctggttgtct ggagattcag ggggccagtc tagcaactaa agcactgccc
       61 gtctcctggt taaccccatg tgctataccg ccggtataga caatcagcga gcctccggcg
      121 ctaatattgc tgccagttgc cgtaccatct ttctcaacaa cctgccggct cccggtggaa
      181 atatttgtcg tgtcagcttt cccgccgctc ttgatatttt gtgttccgct gttgatattg
      241 gtacccgtgg caataccatg attattaata ttctgtgtac caccattgat tatggtattg
      301 gtcgcgtttc tggtaacatc cattaccccg ccgttatcta ttcgggtcgc atcagcttta
      361 gcattcgtta aaactgacat tgttccttta tctttaataa tcgtcttatt tgccgaacca
      421 tatgcgttta tgtctaaatg accaccgttt tccagcaaca cattgtctgc cacgtgattg
      481 tggatggaga atgcaccttc actattcgta ccgctcaccg tcgtaccgtt agtgtttgtt
      541 tttaaaattg caccatcgtg ctgggtaaca tttgttgccg taccaccact aacatcaagc
      601 acgccaccgg aataaacttc aataacatcc gaggagctgg tgtaatcaac aatttgcgta
      661 ccaccagaat agatctgagt attttttgcc gttgacttat tattcagaga ctgagttcca
      721 ccttcaatcg tcgtgtccag cgcatggctt tcatatactc tttgctcacc gccattttta
      781 atggtggttg ttcctactgt gctctgttca acatactgtc gtccaccatt tatgattgtg
      841 ttcgttgcca tacttccttg cgtgatgtcc tgataaccgg acttatttat cgttgtacca
      901 tcaacatgcc cctgagttgt tactgtctgc cctccaccat caatgatggt tccattcgca
      961 gtccccccac ttatgtgtga attaccaccc ttaattgtcg ttccattgct gataccgcct
     1021 gtataaacgt cctgattgcc actgtcgatt atcgtaccgg tggaaatgcc cccgtcatga
     1081 attgtctgtc ttcctccgtt aatggttgta ttattagact gccccatata attgttattc
     1141 cttccaacat cttgatatcc accagattca ataagacttc cattagatac tccgccataa
     1201 acactctgct ggccatggtt gataatatga gtgttatttg ttgtacctcg ttcatctact
     1261 cgttggttgc catctacagt ctcatcgttt accacaccaa taacattagt agtaaaggcc
     1321 gccatcacgg gcggggcata tatcaaggca gatatcaata aggaaagtac tgagcggcga
     1381 caataatggg gactggtcct gttcataaat ttcatcctct gaaaagtgaa tactgagtag
     1441 cgtttaagcg actttagctc tgctgcaaca tcagcccgca ggcaccagac cggggggttc
     1501 atcctgaaga gacagcgcaa gtgtattgtg ttcaccgctc atcaaagaca tcatgatgaa
     1561 atgatgatgt tccacataag aaagaggcat tttttaaaca cagtgcgctg aagtgtgatt
     1621 ggataaaaaa gtcaatctat tcaggaaata cgggcgtatt cttttctttc gacaatcagg
     1681 ccgtcggcaa aataaaatga tttacataat cgtttctgat gaatatcttc tgctcacata
     1741 aaaatcacac aataactttg agatcgcaga ttcttttact tttacagcat tcgtcccccc
     1801 attgttgggc aaatatagat tgggccagag cacgaaagtt aataccacgt ttgcacagct
     1861 cctccaacag cacgacaaga tgccacatac tgcgccccag tcggtgcggt ttacagacta
     1921 cccgtgcgtc caccgccgat aatgtcctga gcagtttttt cagtccggac ctcctgccac
     1981 ggttttacgt tgaagaggta accctgagca cgcagttctt cagtcaggcg tggtgcaccg
     2041 taacgctgtt attgctgggt aagatcaaaa aactttcagg cagctaagga aagttgaacc
     2101 agacattaga tgaaatattt caaccaatta cagcaccaat ttaaactcag taaatcacag
     2161 cacagatcaa ggggttacgt gaaagcgtag cccctttttc tttggtagtg gcagcaaaat
     2221 ggttgtagtg taaaaaataa tcccgtttaa tcaatcaata atacatattg tttcaatcta
     2281 cgttattatc tctttgtaaa aatagccatt tattaatcat tgaaaactgc ttttagaact
     2341 tgatacaacg ggactagtca caacaggact attctcaacg ggatcatcct cagaggaact
     2401 atcatcaaag tcatcatcca taaataaaat atcatcgaat ggtgccacgc ccgtgatgag
     2461 ttttatttta ttattacgat cagtcaagac tccacttaaa ccgttttcgc tcacaggttt
     2521 taatgatttt tcattactct tgttgtaagc aggcgcatta aaaatacacg gagtatcaag
     2581 atcaaacaat gacgttctcc agttcacata ttgaatatca tagttactga agttctgtcc
     2641 agaaaagaag catcccttaa aatccaatcc acctaaatta tataaaccat cctcttcttt
     2701 ttggagagta atgttaattc tggctatctc ccggacaaaa cttccttttt tgtatttgaa
     2761 taccgtttca agattttcac cagacagctt gacttctggg aataatttaa aattaaaccc
     2821 tacatcatta ttattcaaag tagatgaacg aaagatggaa aaagcttgta gtgctgaatt
     2881 atagctttcg attttatctt gaggctgcgc tcttggtaaa aacttatagc agcactcata
     2941 aaaattgaat aaacgttctg aacaattccc atttttatca attaataccc cccggaattc
     3001 tttcacaaag gcatccgtta ttttttgaat atctatggtg tgttcctttg actctgacaa
     3061 agatggagtc tgttgaagtg cattgctcat ctggttaagg aacatctgca tttctttaaa
     3121 tttatctcca tagtggcctt taacttgctg agagttttta gttacaggtg aaaccttatc
     3181 tatttggttg tcatataaat tatgtggttc catacagaca tttggttgtg tttctaaaat
     3241 aacactacgt gtactacctg gcttaaatga atcaacatga aaatcacttt ttcttatatt
     3301 atcgaatagg gtctgttcat ttctcttagc gcattcaata aactgatcaa cattattttt
     3361 attcgatgac aggtcagttt cagaaaccgc attttctttg ttctccagct ttagtttaat
     3421 gagaagatct tcccagacct gcttgcttaa acatactacg tcaggctcac catcagtaac
     3481 taactcttca ttattatcaa agtatacgtt gaatactttt aagttatttt catcaacatc
     3541 atatttaata cgttttaatt gctctccaga tcccataatg a
//
LOCUS       DAEEKQ010000278         3578 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.283, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000278 DAEEKQ010000000
VERSION     DAEEKQ010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3578
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.283"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..197
                     /locus_tag="KLK57_004142"
     CDS             <1..197
                     /locus_tag="KLK57_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894357.1"
                     /translation="SPKKIGFYTIGDKSIYLYDLRRMDEIMEALDNRSSMDWCVAVHD
                     MNAGFDEKILFPSSVESTAG"
     gene            208..375
                     /locus_tag="KLK57_004143"
     CDS             208..375
                     /locus_tag="KLK57_004143"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894358.1"
                     /translation="MTTITKERIELFIKNPLENGLTRGEQMELARIALASLEAEPVVF
                     WFEKYQEGATA"
     gene            483..716
                     /locus_tag="KLK57_004144"
     CDS             483..716
                     /locus_tag="KLK57_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894359.1"
                     /translation="MDIAVLEIALVSLAAEPAGKLHEYKPVGYQRLVDELTMLVKQLT
                     WQLRKAKPDCKLPDKAMSYLERNGLISVEDILR"
     gene            1008..1163
                     /gene="hokD"
                     /locus_tag="KLK57_004145"
     CDS             1008..1163
                     /gene="hokD"
                     /locus_tag="KLK57_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HBE0894360.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            1467..1739
                     /locus_tag="KLK57_004146"
     CDS             1467..1739
                     /locus_tag="KLK57_004146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894361.1"
                     /translation="MPSRLHNSDNSAVFASRHGRRSHAFKSDWYQHPPCTEEQAEWLI
                     QCYRRRGCEVKKALSLDYRHWIISVRLPYSERPTRPSRTFQQRIWR"
     gene            1813..2799
                     /locus_tag="KLK57_004147"
     CDS             1813..2799
                     /locus_tag="KLK57_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HBE0894362.1"
                     /translation="MSAFHNGRILVEPEPKSMRNLPSGVVPAARQPLVEDKTLLPFFS
                     NARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHHSETVIHRYGTGAMVLCWHCDNQLR
                     DQTSESLEQLAHQNLSAWMIDVIGHAISGTQERELSLAELSWWAVRNQVADALPEAVL
                     RRSLGLRAEKIRSVYSESDIIPGEQTATSILKQRTKNIALLPHVHQQQNPPQEKTVVS
                     IAVDPESPAQYLQRQKSQREEMPVYTRWVKTQKCMTCGNQADDPHHIIGHGLGGMGTK
                     ADDLFVIPLCRKCRNELHAGVKDFEEKHGSQLLLLIRFLMHARNSGVLKWKA"
     gene            2800..3180
                     /locus_tag="KLK57_004148"
     CDS             2800..3180
                     /locus_tag="KLK57_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBE0894363.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRAVALIVR
                     QQRLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLMDDEQFDEINIVR
                     AQPVSGGRLGVKIYPIMLEGQVKK"
     gene            3177..>3578
                     /locus_tag="KLK57_004149"
     CDS             3177..>3578
                     /locus_tag="KLK57_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBE0894364.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGVSAFLGKMGISMNDRERATELLTEYALSRCDRVAALRKLPAEIKPAVMRIMASY
                     AFEDYARSAASKKQCPCCHGKKFIESEVFTNK"
BASE COUNT          968 a          809 c          980 g          821 t
ORIGIN      
        1 catcaccgaa aaaaattggc ttttacacca ttggcgataa aagcatttac ctgtatgacc
       61 tacgccgcat ggatgaaatc atggaggctc ttgataatcg ttcgtcgatg gattggtgtg
      121 ttgctgttca tgatatgaat gcagggtttg atgaaaagat tttgttcccc tcatcagttg
      181 aaagcactgc gggttaagga gtaacacatg accactatta ccaaagaacg tattgaattg
      241 ttcattaaaa acccgcttga aaacgggctt acccgtggtg aacaaatgga actggcacgg
      301 attgcgctgg catcgctgga agcagagccg gttgtgttct ggtttgaaaa atatcaagaa
      361 ggggctacgg catgacgact tttaccagag agcagttaat agctcacgca gaggagacta
      421 ttgaagcaca gagactgtgc ataccgggca caatcgacca tgacatcatc cgcacatata
      481 agatggatat tgctgttctg gaaatcgcac tggtatcgct ggcagcagag ccagccggta
      541 aattgcatga atacaaacca gtgggatatc agcgtctggt cgatgagtta accatgctgg
      601 taaagcagtt aacctggcaa ctgaggaaag cgaagccaga ctgcaaatta ccggataagg
      661 cgatgagtta tctggagcgg aacggactga taagcgtgga ggatatttta cgatgacctg
      721 gcctgaagca ttaacaacgg taggaattgc gatggcggtg gcgctggtgg tgtattcgat
      781 ttgccgctgg ggataaaaac ggtttgcggg aaaaggagag ttaagtagaa ttgcagcggg
      841 tgcttgaggc tatctgtctc aggcatgaac accaaaaggc agatagagaa aagccccagt
      901 taacattacg cgtccggcaa gacgcttaac attaatctga ggctcaatct atgaacggca
      961 aatctaggtt agcctcttac gtgccgaaag gcaaggagaa gcaggctatg aagcagcaaa
     1021 aggcgatgtt aatcgccctg atcgtcatct gtttaaccgt catagtgacg gcactggtaa
     1081 cgaggaaaga cctctgcgag gtacgaattc gaaccggcca gacggaggtc gctgtcttca
     1141 cagcttacga acctgaggag taagagacca ggcgggggag aaatccctcg ccacctctga
     1201 tgggtcaggc atcctcaatg cacccacact taacccgctt cggcgggttt ttgtttttat
     1261 tttcaacgcg tttgaagttt tagatggtgc cggaatagaa tcaaaaatac ttaagtagcg
     1321 cgcagggaga agagggatgg accccgaaca ggggagtgct atttatctgg aaggattctg
     1381 ttgatgaaaa tcgaagaatt acgtgaaatt tttagtgaag atggcctcta tactgtgcgc
     1441 gttgagaatg gcgctattgt cagccactgc cttccagact acacaattct gataattcag
     1501 ccgtctttgc cagcaggcac gggcggcgct ctcacgcatt taaatctgat tggtaccagc
     1561 atcccccatg cactgaagaa caggccgaat ggctcattca gtgttaccgc aggcgcggat
     1621 gcgaggttaa aaaagccctt agcctcgact accgtcactg gataatctcc gtcaggctcc
     1681 cttactccga acggccaacg cgtccgtccc gcacattcca gcaacggatc tggaggtaat
     1741 gtgcgggtat tacttcgacc tgttctggta ccggaactcg gtctggttat cgttaagcca
     1801 ggccgtgaat caatgtcagc attccataac ggcagaatac tggtggagcc ggaaccaaaa
     1861 agcatgcgta atctgccgtc cggggtcgtt cctgccgctc gccagccgct ggtggaagac
     1921 aaaacattgc tgccgttttt cagtaacgca cgggtgattc gtgctgctgg tggtgctggt
     1981 gcattgtctg actggctgtt gcgccatatt aaatcctgcc agtggccaca cggcgattat
     2041 catcacagcg aaaccgtcat tcaccgttat ggtaccggcg caatggtgtt gtgctggcac
     2101 tgcgacaacc agctgcgtga ccagacatcc gaatcactcg agcaacttgc tcatcaaaac
     2161 ctgtcagcat ggatgattga cgtcatcggt cacgcaataa gcggtacgca ggagcgtgaa
     2221 ttatctctgg ctgaattatc ctggtgggcg gtccgcaatc aggtggcgga cgcgctaccg
     2281 gaagcggtat tacgtcgttc gctggggttg cgtgcggaaa aaattcgctc tgtgtacagt
     2341 gaaagcgaca tcataccggg agagcagaca gccaccagca tactgaagca gcgcacaaaa
     2401 aatattgcgt tactgcctca cgtccaccag caacagaacc caccacagga aaagacggtg
     2461 gtcagcattg ccgttgatcc ggagtcaccg gctcagtatc tccagcgcca gaaatcacaa
     2521 cgggaagaga tgcctgtata cacgcgctgg gtaaaaacgc agaaatgcat gacgtgtggc
     2581 aatcaggcag atgatccgca tcacatcatt ggtcatggac tgggagggat gggaacaaag
     2641 gctgatgatt tgtttgttat tccgttatgc cgtaaatgcc gtaacgaact acatgccgga
     2701 gtaaaagatt ttgaagagaa acacggcagt cagctgttgt tgctgattcg ttttttaatg
     2761 cacgcgagaa attcgggtgt tctgaagtgg aaagcataaa tgaccgagcg catagaattt
     2821 gttttacctt acccgccgac ggtgaacact tactggcgtc gtcgtggcag cacatatttt
     2881 gtatcaaaag ccggggagcg ttatcgccgg gcagtggcgc ttattgttcg ccagcagcgg
     2941 ctgaaattaa gcctgtccgg aaggttggca ataaaaatta ttgcagaacc accggataag
     3001 cgccgccgtg acctggacaa tattctgaaa gcgccgctgg atgcgctgac gcatgcgggg
     3061 ttgctaatgg acgatgagca gtttgatgaa atcaatatcg ttcgtgctca gccagtatct
     3121 ggtggacgtc tgggggtgaa gatttatccc ataatgcttg aagggcaggt caaaaaatga
     3181 aactggaaga tttaccgaaa tactactccc caaaatcccc cggcctgact gatgcatcgg
     3241 cctcaacgtc gaaagatgcg ctgagtatca ctgatgtgat ggccgcgcag ggcatgacac
     3301 agaatcgggc tgagatgggg gtttctgcgt tccttgggaa aatgggcatt agtatgaatg
     3361 acagagagcg ggcaacagaa ttgctgacag aatatgcact cagtcggtgt gatcgcgtgg
     3421 cggcgttaag aaaactcccg gcagaaataa aaccggcagt gatgcgtatt atggcttcgt
     3481 atgcgtttga agattatgcc cgtagcgcgg cgagcaaaaa acagtgcccc tgctgtcacg
     3541 gaaaaaaatt tattgaaagc gaggttttta caaacaag
//
LOCUS       DAEEKQ010000279         3559 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.284, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000279 DAEEKQ010000000
VERSION     DAEEKQ010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3559
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.284"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..849)
                     /gene="melB"
                     /locus_tag="KLK57_004150"
     CDS             complement(<1..849)
                     /gene="melB"
                     /locus_tag="KLK57_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HBE0894365.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTL"
     gene            complement(952..2307)
                     /gene="melA"
                     /locus_tag="KLK57_004151"
     CDS             complement(952..2307)
                     /gene="melA"
                     /locus_tag="KLK57_004151"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HBE0894366.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALQDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYLHIKQVGLCHSVQGTAEELARDLNIDPSTLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            2590..3498
                     /gene="melR"
                     /locus_tag="KLK57_004152"
     CDS             2590..3498
                     /gene="melR"
                     /locus_tag="KLK57_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HBE0894367.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGNITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
BASE COUNT          969 a          930 c          834 g          826 t
ORIGIN      
        1 taacgtcacc aggttagcag ctcccgcata cgacagataa taggggaaca aatccgcatc
       61 accgataaca tatgagaaat aatagatagc aaatccggta ataatgttgc tggctacatt
      121 ataagcaaga gccataccca agaggcatga aagctgatcg tttttataaa ttagcgcaac
      181 gatggcttta agtgtcagat ggcttccttc agcagacggt tgattgtctg acgaaaagac
      241 ttcatgcaca ttgcgcagag tgatgatggt tgaaacaata aaaaaggcga tcagtaccag
      301 tgtgaacatc tgaaagccaa atccccgatc gccaccgccg acataattaa caaatggtag
      361 cgtcacacct gccgtaacaa agcccgccag actggcaaaa aaacgcggat aaggaaccag
      421 ttgttcacgc tcacgtttat cgagcgtgat ggttggaacc agcgaccaga agggaatatc
      481 cataatggtg taagtcatgc cccagaggat gtaggtcacg caaacaaaga caatctgagt
      541 agtaccttca aacagatgcg cactaaagag gagaaataag attacagagt ttgccaacgt
      601 accgatcagg atccagggtt tgaacttacc ccatcgcgat cgcgtagcat ttacaatcca
      661 tcccataatc ggatcgttaa tagcatccca gatcctcgcc accagaaaca aagtaccgac
      721 caaacccaca gacagcccaa cgacatcggt gtagtaatac atgaggtaca tatacacaat
      781 gccgatcgca aaatccttcc cgaacgctcc aaatccataa ctgagttttg tagtcattga
      841 aatgctcata gggtatcggg tcgctgttac caacgcccgc cttctgttat tggtatcgtc
      901 agaggtgtgc gccggaataa atcccccggc gcagtagcgt ttagtcgcgt tttaacggtg
      961 caaccagcct ggcagccagt cgccgtgggc ggcaatcagg tcgtcaacaa gagcatatat
     1021 ttcgtcaatg cccagcacgg cggcagtatg cgggtccatc atcgcggcgt ggtaaacacg
     1081 gtcgcgattt tccgtaagaa tagcttcggt cagcagcgtc tgtacattga tgttggtttg
     1141 catcagggcg gccagatgcg aaggtagcgt accgacttta gtcggctgaa tgccattagc
     1201 atcaaccaga caggctactt ccacgcaaca tccttgtggc aggttatcaa tcaaaccatc
     1261 gttacggacg ttgccgtaaa tcacactcgg ctcgccagtc cagatagcgt tcatgattgt
     1321 gctggcatat tctcgtgacg gtttaatatc aatccgggag gcgtttttat actcctccag
     1381 ctctttatgc cagttcgcca gctgctcgac gcagcgtttc gggtactcat ccagcggtac
     1441 tttataacgc tcaatcaaat cctcacgacc tggcttaata aaccacggcg tgtattcagc
     1501 aaaatgttct gacgattccg taacgaagta gcccagcttt ttgaacattt cgtagcgcac
     1561 aatattctgg cagcgagtat taccatgaat atttggcttc ggtgcctgcc ctgcttcata
     1621 agccgccagc agttccgggt agagattcac gtaactgccg tcggcggttt tacgctccag
     1681 ttccaggtaa aacgccatat ggttgatacc agcgcaacgg taacgcagcg tggacggatc
     1741 gatattgagg tcacgcgcca gctcttccgc cgttccctgc accgaatggc acagccctac
     1801 ctgtttgata tgcagatagc gggcatacat cgcccaggta ttcatcgcca ttgggttaac
     1861 gtagttgagc atggtggcat cggggcagac ttccgtcatg tcctcgcaaa tttgccacag
     1921 atgcggaatg gtacgtagcg cgcgcataat accgcccggc cccaacgtat cggcaatggt
     1981 ttgttccaga ccatgccgct tacagacctc gaaatcagtc accgtgcaag gttcataacc
     2041 gccaatctga aatgccacca cgacaaaatc ggcatcctgt aaggcttctt tctgttgggt
     2101 gtggcaggtg attttgccgc tggcccctgc tgaatccatc agcttacgca ccacaatatg
     2161 cgactcttcc aggcgggtgg gatcaatgtc catcagggca atatgcgccg ttttcagcgc
     2221 ctcgcgatgg aacacatcac caagaatatt tttaacgaaa atcgtcgaac cagcgccgat
     2281 aaatgtaatt ttgggtgcag tcatcatgca gatctcctgg cttgcttgaa taacttcatc
     2341 atggcaggcg aatctgcaga aaacctccgg cttccaggtt gagcattccc ataaactcag
     2401 atcctgcgtg aagcagcagt aaatctgagt ttatggtaat aagttacctg aaaagcagag
     2461 ttttcggcct catgatgctc ggacgctccc ctcgcctctc tgggaagagg gtgaaaaccg
     2521 tgctcccact cgcagtcatc ctccctcact cctgccataa ttctgatatt ccaggaaaga
     2581 gagccatcca tgaatacaga tacgtttatg tgcagcagcg acgaaaaaca gacccgcagc
     2641 ccgctgtcgc tgtattcgga ataccagcgg atggagattg agtttcgcgc accgcatatc
     2701 atgcccacca gccactggca tggtcaagtc gaagtgaatg tgcctttcga tggcgatgtg
     2761 gaatacctga tcaacaatga aaaagtgaat atcaatcagg gtaatatcac gctgttctgg
     2821 gcctgtacac cgcaccaact aacagatacc ggaacctgtc agagcatggc gatttttaat
     2881 ctgccgatgc atctgtttct ctcctggccg ctggataaag acctgattaa ccacgtcact
     2941 cacggcatgg tgatcaaatc actggccaca cagcaactta gcccgtttga agtgcgccgc
     3001 tggcagcagg aattaaacag tccgaacgag caaattcgcc agctcgccat tgatgaaatt
     3061 ggcctgatgc tcaagcgatt tagcctctct ggctgggaac cgattctggt caataaaacc
     3121 tcgcgcacac acaaaaacag cgtctcgcgc catgcgcaat tttatgttag ccagatgctg
     3181 ggctttattg ccgaaaacta tgatcaggcg ctgaccatca acgatgtggc tgagcacgtc
     3241 aaacttaacg ccaactatgc aatggggata ttccagcggg tcatgcaatt gacgatgaaa
     3301 cagtacatta ccgcgatgcg catcaaccac gttcgcgcgt tactgagcga taccgataaa
     3361 agtattctcg atattgccct gacggcaggc tttcgttcaa gtagtcgttt ttacagcacg
     3421 ttcggcaaat atgtcggcat gtcgccgcaa caataccgca aacttagcca acagcgccgc
     3481 cagacgtttc ccggctaaag acgatatcag tatcagccaa aaaaatagtt tagccgtcga
     3541 taataagcag tgaaaataa
//
LOCUS       DAEEKQ010000280         3551 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.285, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000280 DAEEKQ010000000
VERSION     DAEEKQ010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3551
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.285"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..127
                     /locus_tag="KLK57_004153"
                     /pseudo
     CDS             1..127
                     /locus_tag="KLK57_004153"
                     /inference="COORDINATES: protein motif:HMM:NF019996.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
     gene            complement(409..645)
                     /locus_tag="KLK57_004154"
                     /pseudo
     CDS             complement(409..645)
                     /locus_tag="KLK57_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            918..1130
                     /locus_tag="KLK57_004155"
     CDS             918..1130
                     /locus_tag="KLK57_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894368.1"
                     /translation="MRRPDRRQQRRAAPRRHPRRRQRLLVVRRQQLRAKVRRNLQQRA
                     LRQRQNGQRILHPPWRLRMRARRKRG"
     gene            1079..1231
                     /locus_tag="KLK57_004156"
                     /pseudo
     CDS             1079..1231
                     /locus_tag="KLK57_004156"
                     /inference="COORDINATES: protein motif:HMM:NF015371.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1208..1387
                     /locus_tag="KLK57_004157"
                     /pseudo
     CDS             1208..1387
                     /locus_tag="KLK57_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003910815.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            1526..2257
                     /locus_tag="KLK57_004158"
                     /pseudo
     CDS             1526..2257
                     /locus_tag="KLK57_004158"
                     /inference="COORDINATES: protein motif:HMM:NF019126.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            2658..2798
                     /locus_tag="KLK57_004159"
                     /pseudo
     CDS             2658..2798
                     /locus_tag="KLK57_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247236.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            2809..3390
                     /locus_tag="KLK57_004160"
     CDS             2809..3390
                     /locus_tag="KLK57_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE0894369.1"
                     /translation="MAFRMSEQARTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDESSWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEFQKWNGTAWVKDTEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEKE
                     TLLLEAWKKYRVLLNRVDTSTAPDIEWPVAPIG"
BASE COUNT          974 a          917 c          986 g          674 t
ORIGIN      
        1 catgacggag gatgatgccc gtccggaggc actgcgccgt tttgagctga tggtggaaga
       61 ggtggcgcgt aacgcgtccg cggtggcaca gaacacggca gccgcgaaga aatcagccag
      121 cgatgccagc acatcagccc gtgaggcggc aacccatgcg actgatgctg cagattcagc
      181 acgtgcagcc agcacgtcag ccggacaggc cgcttcgtcg gctcagtcag cgtcttccag
      241 cgcaggaacg gcatcaacaa aggccactga agcctcaaaa agtgctgccg ctgcagagtc
      301 ctcaaaaagc gcggcggcta ccagtgccgg tgcggcgaaa acgttagaaa cgaatgcggc
      361 agcgtcacaa caatcagcag ccacttctgc atccaccgcg accacgaaag cgtcagaagc
      421 tgccacctca gcccgggatg cgtcggcttc aaaagaggcg gcaaaatcat cagaaacgaa
      481 cgcatcatca agcgccagta gcgcagcttc ctcggcgacg gcggctgcaa attctgcgaa
      541 ggcggccaaa acgtccgaga cgaacgccag gtcttctgaa acggcagcgg gacagagtgc
      601 ctcagctgcg gcaggctcaa aaacagcggc tgcgtcgtct gccagcgccg cgtcaacaag
      661 tgccgggcag gcctcagcca gtgccaccgc cgccggaaaa tcggcagaaa gcgccgcatc
      721 atccgcttca acagccacaa cgaaggctgg cgaagccact gagcaggcca cggcagcagc
      781 gaggtcagct tccgcagcga agacatccga aacgaacgcg aaagcgtcgg aaacccgtgc
      841 agaatcctca aaaacggctg ccgcatcgtc cgccagttcg gcggcgtcat cggcatcatc
      901 ggcgtctgct tcaaaagatg aggcgaccag acaggcgtca gcagcgaagg gcagcgccac
      961 gacggcatcc acgaaggcga cagaggctgc tggtagtgcg acggcagcag ctcagagcaa
     1021 aagtacggcg gaatctgcag caacgcgcgc tgagacagcg gcaaaacggg cagaggatat
     1081 tgcatccgcc gtggcgcttg aggatgcgag cacgacgaaa aaggggatag tacagctcag
     1141 tagtgcgact aacagcactt ccgagtcact ggcggcaaca ccaaaagccg ttaaggccgc
     1201 gtatgacctg gctaacggga aatacaccgc acaggatgca acgacagcac agaaagggat
     1261 aatccagcta agcagcgcga ccaacagcac gtctgaaacg ctggcggcaa cgccaaaggc
     1321 agtaaaagca gccaatgaca atgctgagaa acgtctgcag aaagatcaga acggtgcgga
     1381 tatccctggc aaagacacct ttacgaaaaa tattggtgcc tgccgtgcct tcggtgggtc
     1441 agtaagcaca acaacaggaa actggacgac tgcacagttt atcgagtggc tggattctca
     1501 gggagcattt aaccatccat actggatgtg caagggttcc tggtcttatg gcaataataa
     1561 aatcattact gatactggct gcggtaatat tcatctcgcc ggagctgtca ttgaagtaat
     1621 ggggataaag tcagcgatga cgatccgcat taccactccg accacatcca gcggtggcgg
     1681 aacaaccaac gcccagttta cctatattaa tcacggaaca gattattcac ctggctggcg
     1741 aagggactat aactccagaa ataagccaac ggcatcagag atcggggcgc taccgtcagg
     1801 tggaacagca gtatcatcag ttaatctggc ttcaaaaggt cgggtagccg cgctgacaga
     1861 caatacgcag ggggcagcag gtcttgagtt atacgaggtg tataacaacg gatatccaac
     1921 agcgtatgga aatatcattc acctgaaagg gatgacagcc gttggcgaag gtgagttact
     1981 catcggctgg agtggtacaa gcggtgctca tgctccggca tttattcgtt cacgacggga
     2041 tacgaccgac gcaaactggt cgccgtgggc gcagctttac acctcggctc atcctcctgc
     2101 agagttttat ccagtcggtg caccaatccc gtggccatca gataccgttc cgtctggtta
     2161 tgccctgatg caggggcaga cttttgacaa atctgcttac ccgaaacttg cagccgctta
     2221 tccgtcaggc gtgatccctg atatgcgtgg ctggacgatt aagggcaagc ccgccagtgg
     2281 tcgtgccgta ttgtctcagg aacaggatgg cattaaatcg cacacccaca gcgccagcgc
     2341 atccagtacg gatttgggga cgaaaaccac atcgtcgttt gattacggca ctaaatccac
     2401 gaataacacc ggggcgcata cgcacagtct gagcggctct acaagttcag ctggggccca
     2461 ccaacatagt cagactgggc ccagaacgaa tagcgggagt caacctactg gaatgtttcc
     2521 ggcagggagt actcaagtta gcgggactaa tcaggttggt atcagtggct cattaaccag
     2581 tggcacctcc caatgggtag gtaaatcatc ttcagaagga aaccatacac actcactgtc
     2641 cggcacagca gcatctgcgg gtgcacacgc gcatactgtc ggtattggtg ctcatacgca
     2701 ctctgttgcg attggttcac atggacacac catcaccgtt aacgctgctg gtaacgcgga
     2761 aaacaccgtc aaaaacatcg catttaacta tattgtgagg ctcgcataat ggcattcaga
     2821 atgagtgaac aagcacggac cataaaaatt tataacctgc tggccggaac taatgaattt
     2881 attggtgaag gtgacgcata tattccgcct catacaggtc tgcctgcaaa cagtaccgat
     2941 attgcaccgc cagatattcc ggctggtttc gtggctgttt tcaacagtga tgagtcatcg
     3001 tggcatctcg ttgaagatca tcggggtaaa acggtttatg acgtggcttc cggcgacgcg
     3061 ttatttattt ctgaactcgg ctcattaccg gaaaatgtca cctggttatc cccggaaggg
     3121 gagtttcaga agtggaacgg cacagcctgg gtgaaggata cggaagcaga aaaactgttc
     3181 cggatccggg aggcggaaga aacaaaaaac agcctgatgc aggtagccag tgagcatata
     3241 gcgccgcttc aggatgctgt ggatctggag atcgcaacgg agaaagaaac cttgttgctg
     3301 gaagcttgga aaaagtatcg ggtgttgctg aaccgtgttg atacatcaac tgcaccggat
     3361 attgaatggc cagtagcacc tatagggtaa attcgtaatg attacctaaa tacgttattc
     3421 ttttgttaaa aaagtgattt tgctataggt agggagtatg ttcttggatt aagaataaaa
     3481 aatcgccatg aaagagcttc atggcgagta cataaacgta caaaatgtga tgaaagtaga
     3541 gtggcgtaag c
//
LOCUS       DAEEKQ010000281         3546 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.286, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000281 DAEEKQ010000000
VERSION     DAEEKQ010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.286"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..143)
                     /locus_tag="KLK57_004161"
     CDS             complement(<1..143)
                     /locus_tag="KLK57_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894370.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVL"
     gene            complement(189..530)
                     /locus_tag="KLK57_004162"
     CDS             complement(189..530)
                     /locus_tag="KLK57_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894371.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(699..890)
                     /locus_tag="KLK57_004163"
     CDS             complement(699..890)
                     /locus_tag="KLK57_004163"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894372.1"
                     /translation="MGPALGAARLAQIAANPEKSLIELLPQLPLEQSHLPDAQRYAAY
                     QPRRETFRRLYQQLLPLMA"
     gene            complement(823..2154)
                     /gene="xylB"
                     /locus_tag="KLK57_004164"
     CDS             complement(823..2154)
                     /gene="xylB"
                     /locus_tag="KLK57_004164"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HBE0894373.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVDSQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESVEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQKLDYRTGGGCGASTGRSKAGADCGESREIAH"
     gene            complement(2226..>3546)
                     /gene="xylA"
                     /locus_tag="KLK57_004165"
     CDS             complement(2226..>3546)
                     /gene="xylA"
                     /locus_tag="KLK57_004165"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HBE0894374.1"
                     /translation="QAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRFA
                     ACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHDV
                     DVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDPE
                     VFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVEH
                     KHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAGH
                     SFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTGG
                     LNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNSE
                     LGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
BASE COUNT          924 a          983 c          784 g          855 t
ORIGIN      
        1 agtacggcaa aataataccc tttttcattt aactgcatct ctgcattcca tagccctaac
       61 agataggtaa cgataccacc aacgagagct atccatgaca caatactaaa ggccggtgaa
      121 taggttgata ttttgttgtc catcacagta ttcctttcat tcctgaatgt gtaagagctt
      181 tatgttgctc ataccgagcg aaacaaacgc atttgaccaa acaagcagac aaaaaaggcg
      241 atggggtaga tcaccatttc ataagcattg ataggtgcat ttaaaacacc gataaacaag
      301 agaccgatag tgattaacgc aactaactgg cagatatggg taaaaaaatt atccagttgc
      361 ccgagttttt ctgcgcgaag atatgcatag ttaccaaaag ttgctgtcat taacacgcca
      421 agaaaatagc cttttccact tagcaatggg catgagatcc atagcccaac cagataaacc
      481 agcgccccga caacaaataa tagttttgcc acagtttttg aggttttcat aacgatctcc
      541 atatctacca gcgatacatt acgagtaacc aacgaaagac aaaactgaag aatgccatta
      601 acaaatgatt ttcagaataa attcatacta aatattaatt aattagtgag atatatagat
      661 gtgaattatc ccccacccgg tcaggcaggg gataacgttt acgccattaa tggcagaagt
      721 tgctgataga ggcgacggaa cgtttcacgt cgtggctgat aagcggcata acgctgcgca
      781 tctggtagat gcgactgttc taacggtagc tgcggcaaca actcaatgag cgatttctct
      841 ggattcgccg caatctgcgc cagccttgct gcgcccagtg ctggccccac atcccccccc
      901 cgtacggtaa tcgagcttct gaccgctgat atccgccagc atctgacgcc agtactcact
      961 acgcgccccg ccaccaatca acgtaacact ttgcggttta atgccgcagg catgcacgac
     1021 atccatgcca tctgccagcg cataacccac gccttccagc actgctcgcg ccagttcatt
     1081 ggggccatgt tgatgagtca aaccaaagaa aacccccttc gcctggggat tattgtgtgg
     1141 tgtacgctcg ccggaaagat aaggcagaaa ccaaactggc tcgacacttt catcagcctg
     1201 ttgagctgca gcgattaaag ctgggacatt gcacaggccg gttaatttcg cggcccaatc
     1261 cagacacgat gctgcactca gcattacaga cattaaatgc caacgctgcg gtagcgcatg
     1321 gcaaaagctg tgtacagcgc tttctggctt gcttaagaac ccttcgctga cagcaaaata
     1381 gacccccgac gtccccagcg ataacattgc ctgattagca tcaaccattc ccacaccaac
     1441 tgcaccagct gcattgtcgc caccgcctgc gacaactggc accgtcgcca taccccacgc
     1501 tttcgcaact tcaggtaaca aagcaccagt aatttcgctg ccttcgtata atgcgggcat
     1561 ctggtcacga gataagtcgc aagcctgcag catgacgtca ctccagtcac gctttgcgac
     1621 atccagccac atggtgccag ctgcgtcaga catatcgctg gcaaactccc ccgtcatacg
     1681 cagacgcaag taatctttcg gtaataatac tttgtcgatt tgacggaata tctccggctc
     1741 atgccgctga acccatagca atttaggcgc agtaaatccg ggcatcatca ggttgccggt
     1801 aatcactcgt gattgcggaa ctctcgcttc cagcaaagtg cactcttgcg cacagcgccc
     1861 gtcgttccac aaaatggcag ggcgcaatac ccgttgttga gcatccagta aggttgctcc
     1921 atgcatctgg ccggcaatac ccaatgcttt aacgtcctgc agagaatgct gatcgcccag
     1981 agctttcatt gcgcgatcag ttgcctgcca ccactgttcc gggtcttgtt ccgaccagag
     2041 tggatgcggg cgcgaaacgg tcagcttttc cgtttgcgaa tcaaccacct caccctgctc
     2101 gttgagcaaa ataactttta cgcccgaggt gccaagatct atcccgatat acatatcgat
     2161 cgttccttaa aaaaatgccc ggtatcgcta ccgataaccg ggccaacgga ctgcacagtt
     2221 agccgttatt tgtcgaacag ataatgattt accagatttt ccagttgctc ctggcgacca
     2281 ctctgatgca ccggagacaa attatgttcc tgagcatatt tggctaaatc tgccagtgac
     2341 atttggcctt tcaggatttg ctggcccaat tcgctattcc agccggaata acgctgcgcg
     2401 atgcgtttat ccagctcgcc atcttcaatc atgcgcgctg caattttcag cgccagtgcc
     2461 atcgtatcca tcgcgccgat atgaccgtaa aacagatcat atttatcagt actttgacga
     2521 cgtactttgg catcgaagtt cagaccaccg gtggtgaaac cgcctgcttt gagaatttca
     2581 tacatcacca gcgcattctc ttccacactg ttcgggaact ggtcggtgtc ccagcccagt
     2641 tgcgcatcgc cacggttggc gtcgacagaa ccgaacaggc caagcgcaat ggcggtggct
     2701 atttcatgat ggaaagagtg acctgccagc gtcgcgtggt tagcttcaat gttcagttta
     2761 atctcttttt ccagaccaaa ctgtttcagg aagccataga ccgtcgcggc atcgtaatca
     2821 tattgatgtt tagtcggttc ttgcggtttc ggttcgataa gcaacgtgcc ctggaagccg
     2881 attttatgtt tatgctcaac caccatctgc ataaagcggc ccagttgttc acgctcctga
     2941 cgcaagtcgg tatttaacag cgtttcgtaa ccttcacgac caccccacag gacatagttt
     3001 tcaccgccca atttatgggt tgcttccatc gctgtaacaa cttgcgttgc cgcccagctg
     3061 aagacttcag gatctgggtt cgtcgccgca cccgcgccat agcgagggtt tgtaaagcag
     3121 ttagcggttc cccacagcag cttcacgccg ctctcttctt gcttggctgc caggacatca
     3181 accatttgcg caaaattatt gatgtactct tttaacgacg cgccctcagg ggaaacatcc
     3241 acatcgtgga agcaataaaa tggcacatgt aacttgtgga aaaactcaaa tgcgacatct
     3301 gctttacgct tcgccaacgc cagtgcctca ccaggctgct gccacggacg attaaacgcc
     3361 cccacaccaa acatgtccgc cccgttccag cagaaggtgt gccagtagca ggcggcaaaa
     3421 cgcaagtgct cttccatacg cttacccaac accagttcgt cgggattgta gtgacggaat
     3481 gctaacgggt ttgaggattt tgagccttca taacgaacgc gatcgagctg gtcaaaatag
     3541 gcttgc
//
LOCUS       DAEEKQ010000282         3506 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.287, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000282 DAEEKQ010000000
VERSION     DAEEKQ010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.287"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1523)
                     /locus_tag="KLK57_004166"
     CDS             complement(<1..1523)
                     /locus_tag="KLK57_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HBE0894375.1"
                     /translation="MKKAPNLKHQPRDKMTEVIIFAGNDAWAHAKQWQEQDGRLAGDS
                     VPPVWLGEQQLAELDKLQIVPDGRYRVRLYQAGLLRPGLVNTIGQKLAAAGVRDADYY
                     PEGMHSQKRENWREYLERERAEQAEKKKVVELPVKKKERVKDDNASSLALNQMGASQR
                     GEVLLAHYGGELAIHADSDTVHHYNGVVWEPVQDKELQRAMAQIFIDAEISYSQNAIK
                     SAVDTMKLSLPVMGNTARNLIGFSNGVFDTRTGNFREHNKNDWLLIASELPFSPPAEG
                     ETLATHAPNFWKWLRRSVAENDRKADRVLAVLFMVLANRYDWQLFIEVTGPGGSGKSV
                     MAEICTMLAGKANTVSASMKALEDARERALVVGFSLIIMPDMTRYAGDGAGIKAITGG
                     DKVAIDPKHKAPYSTRIPAVVLAVNNNAMSFSDRSGGISRRRVIFNFSEVVPENERDP
                     MLAEKIEGELAVVIRHLLTRFSDQDEAKRLLYEQQKSEEALVIKREGDSLVDFCGYL"
     gene            complement(1520..1819)
                     /locus_tag="KLK57_004167"
     CDS             complement(1520..1819)
                     /locus_tag="KLK57_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HBE0894376.1"
                     /translation="MRTYLSGLTASGYAHPKIIPGAIYLDKNGNRVTVKELMFDRVYF
                     IRDGYSFHSSLNVEIFISRFRREIPLSRNNHVSCMDVDKKLQELKNMIAAWREQK"
     gene            complement(1826..2146)
                     /locus_tag="KLK57_004168"
     CDS             complement(1826..2146)
                     /locus_tag="KLK57_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894377.1"
                     /translation="MPDMTNYQYLINPHFNCEHDIAKKVYSAADGATDNISMAVASIG
                     SLMWHASENEEYDEKAMRIDMGNIGLLLAMLGQFDISLRCTIENATDALNAIEKANTD
                     SNLR"
     gene            complement(2139..2336)
                     /locus_tag="KLK57_004169"
     CDS             complement(2139..2336)
                     /locus_tag="KLK57_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HBE0894378.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARN
                     RCPSGCVAVFAARIRQEVCHA"
     gene            2390..2842
                     /locus_tag="KLK57_004170"
     CDS             2390..2842
                     /locus_tag="KLK57_004170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894379.1"
                     /translation="MRGFVVSIAELHVSALVVFTLHLGEYHFKSFLVFTFVLHCIPLL
                     MGGANMSRSSVNNKSQQLNARFPHEVVSGIEASLQPGETKANFIVTAVRGEIARRQAE
                     GSGENPLVSSLDALANVEQIGIKAAEEIGQLITVAREELQRRKAKESE"
     gene            2900..3076
                     /locus_tag="KLK57_004171"
     CDS             2900..3076
                     /locus_tag="KLK57_004171"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894380.1"
                     /translation="MTNEESTKKTSPARKRRRRKKEHESERFAPCSFALEKYLKEYSS
                     KERASQLWQRTETD"
     gene            complement(3188..3397)
                     /locus_tag="KLK57_004172"
     CDS             complement(3188..3397)
                     /locus_tag="KLK57_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family phage regulatory protein"
                     /protein_id="HBE0894381.1"
                     /translation="MREINEDRVIREDECRKLTGVCRTTRYELEKKGRFPSRLNLGGR
                     SVGWLLSEVMEWVKSRDRINSGKAV"
BASE COUNT          880 a          939 c          744 g          943 t
ORIGIN      
        1 atgagatagc cgcagaagtc caccagcgaa tcgccctcgc gttttatcac cagagcttct
       61 tctgatttct gctgctcata cagcaggcgt ttagcttcgt cctggtcaga aaaacgagta
      121 agcaggtggc gaatcacaac cgccagctct ccttctattt tttctgccag catggggtcg
      181 cgttcgttct ccggtacaac ttccgaaaaa ttgaatatca cccgacgacg tgagatcccc
      241 ccgctgcggt cactgaatga catggcgtta ttgttaaccg ccagcactac tgccggaata
      301 cgcgttgagt agggggcttt gtgtttcggg tcaattgcca ccttgtcacc gcctgtaatg
      361 gccttaatcc ctgccccatc accagcgtag cgggtcatat ccggcatgat aatcagcgaa
      421 aagccaacca ctaacgcgcg ttcccttgca tcttccagcg ccttcatgct tgccgatact
      481 gtgttggcct tacccgccag catggtgcaa atctccgcca tcacgctttt accacttccc
      541 cctggacctg ttacctcaat gaataactgc cagtcgtacc ggttcgccag caccatgaat
      601 aatacagcca gtacgcgatc cgccttgcgg tcattctcag ccaccgaacg gcgtaaccac
      661 ttccagaaat tcggcgcatg tgttgccagc gtttccccct ctgctggtgg gctgaaaggt
      721 aattcactgg caattaacaa ccagtcgttt ttgttatgct cccgaaaatt acctgttctg
      781 gtatcaaata ccccgttact gaatccaatc aggttacggg ctgtattccc cattacaggc
      841 aaacttaact tcatggtatc gaccgccgat ttaatggcgt tctgcgaata gctgatctcc
      901 gcatcaatga aaatctgtgc catagctcgc tgtaattctt tatcctgtac tggctcccat
      961 acaacgccgt tgtaatggtg aacagtgtca gagtcagcat gaatcgccag ttcaccgcca
     1021 taatgtgcca ggagaacttc gccgcgttga cttgctccca tctggttaag cgccagtgat
     1081 gaagcgttat cgtcttttac ccgctctttt ttctttacag gcagttcaac tacctttttc
     1141 ttttccgcct gctctgcccg ttcacgttcc agatattcgc gccagttctc ccgtttctgg
     1201 ctgtgcattc cttcagggta ataatcagca tccctgacac ctgccgctgc cagtttctgc
     1261 ccgatggtat taacaagccc cggacgcaat aaccccgcct ggtagagacg cacccgatag
     1321 cgtccgtccg gtacgatttg cagcttatcc agttcggcaa gttgttgttc tccaagccag
     1381 acaggaggca cgctgtcgcc agccagtcgc ccgtcctgtt cctgccactg cttcgcatgt
     1441 gcccacgcat cattacccgc aaaaatgatg acttccgtca ttttgtcacg cggctggtgt
     1501 tttaaatttg gcgctttttt catttctgct ctctccacgc ggcaatcatg tttttcagtt
     1561 cctgtagttt tttatcaaca tccatacatg acacatggtt atttctggaa agcgggattt
     1621 cccgcctgaa tctgctaata aagatctcca cgttcagcga actatgaaat gaatagccat
     1681 cacgaataaa atacacacgg tcaaacatca gttcttttac cgttactctg ttaccgttct
     1741 tatccagata aatagcgccg gggataattt tggggtgtgc ataaccgctg gcagtcaagc
     1801 cagataaata cgttctcatg attatttatc tcagatttga atcagtattc gctttctcta
     1861 tggcatttaa tgcatctgtg gcattttcaa tggtgcaccg taacgaaata tcaaactgcc
     1921 caagcattgc cagtaacaag ccaatattac ccatgtcaat gcgcatagcc ttttcgtcat
     1981 attcctcatt ttctgacgca tgccacatca ggctaccaat tgacgcaaca gccattgata
     2041 tattgtcagt agccccatcc gcagcggaat aaaccttttt agcaatatca tgctcacagt
     2101 taaaatgcgg attaatcagg tactggtaat tggtcatgtc aggcatggca cacctcctga
     2161 cgaatacggg cggcgaatac agcaacacaa cctgacgggc aacggttacg cgcttcgcgt
     2221 tccgtccagg cggttacgtg gatgatttga gattctccgg cactcagggc cagaaaacgc
     2281 cacacaaagg ccgtttgtgt gtgtacaagg tgtggtatat gatttacggc aaccataacg
     2341 gctcctcgtt tacgttgttg gttagaagcc ctgcgagtgg taacgacact tgcggggctt
     2401 cgtcgtttca attgctgaat tgcatgtatc agcacttgtg gtattcacat tacatttagg
     2461 tgaataccat ttcaagtctt ttttggtatt cacttttgta ttacactgca tcccgttatt
     2521 aatgggaggt gcaaacatgt ccaggagttc tgttaacaat aagtcacagc aactgaatgc
     2581 cagatttcca catgaagtag tgagtggcat tgaggcatcc ctacagccag gggaaactaa
     2641 agcgaatttt atagttacgg ctgtacgcgg tgagatcgcc aggcgccagg cagaaggaag
     2701 tggagaaaat ccccttgtgt cttcactgga tgccctagct aatgtcgaac aaatcggcat
     2761 caaggcagcg gaggaaatcg ggcagcttat caccgttgca cgcgaagaac tccagcgccg
     2821 caaggccaaa gaatcagaat aatcactatc agcgccgtgg tgtgaggaac tccggcgcat
     2881 tgctttacag gcgcacacaa tgaccaacga agaatcaacc aaaaaaacat cacctgcacg
     2941 aaaaagacga cgcagaaaga aagagcatga atcagaacga ttcgcgccgt gttcttttgc
     3001 ccttgagaag taccttaaag aatactcatc aaaggagaga gcttcgcagt tatggcaacg
     3061 cactgaaaca gactgatagc attgcccacc agcctgatag cggctatcat tgacatgctt
     3121 atgtttagtg tttccaaact ggcgaccgcc ccaggtcgcc tttgttttat gtgtcatatg
     3181 ctccccttta cactgcctta cctgaattaa tgcgatcccg gcttttaacc cattccataa
     3241 cctcggacag cagccaccct acagaacgac cgcccagatt aagacgtgac ggaaagcgcc
     3301 cttttttctc cagttcgtaa cgtgtagtgc ggcacactcc agttaactta cgacattcat
     3361 cctcacggat tacgcgatct tcatttattt cacgcataca aaaacctaca taaaaattac
     3421 gtatataaac ttttctctta gctgtagata tatgagatcg gatattactt agattctttt
     3481 tcacctctta aattaaaaac acaacc
//
LOCUS       DAEEKQ010000283         3444 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.288, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000283 DAEEKQ010000000
VERSION     DAEEKQ010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3444
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.288"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..324
                     /locus_tag="KLK57_004173"
     CDS             <1..324
                     /locus_tag="KLK57_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0894382.1"
                     /translation="FYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGKATGIYYS
                     AGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTRHHSLKEN
                     VAPGG"
     gene            551..1816
                     /locus_tag="KLK57_004174"
     CDS             551..1816
                     /locus_tag="KLK57_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0894383.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMTSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFSRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            1828..2694
                     /gene="ydiB"
                     /locus_tag="KLK57_004175"
     CDS             1828..2694
                     /gene="ydiB"
                     /locus_tag="KLK57_004175"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HBE0894384.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEVLASADILTNGTKVGMNPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            2725..>3444
                     /gene="aroD"
                     /locus_tag="KLK57_004176"
     CDS             2725..>3444
                     /gene="aroD"
                     /locus_tag="KLK57_004176"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HBE0894385.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVN"
BASE COUNT          871 a          768 c          843 g          962 t
ORIGIN      
        1 ttttatgtgg tgataatatt tgcttttgtc attggtttta cctctgctgg aggtgttgtg
       61 caaattggcc tgacgttaat ggctgaacgt ttcccttacg ctaaaggtaa agctacaggg
      121 atctattaca gtgcgggcag tattgcgacc tttactattc cgttgattac ggctcatctg
      181 tcccaaagaa gtattgccga tattatgtgg ttcgataccg ccatcgctgc catcggtttt
      241 ttactggcac tgtttatcgg cttacgcagc cgcaaaaaaa cgcggcatca ctcgctaaag
      301 gaaaatgtcg ctccgggtgg gtaatgcaat attcttttca ggtcatgcaa gatcttacgg
      361 ataaataact ctttctgcgc taactaagga aaatcgcgat caaaaacaaa ctatgacatg
      421 caatattcct ggaaacataa actttatgcc atgtacccag ggaaaatcat cttcagtata
      481 gtaattatgt aaaccgtcgg agaacaatac gtacggtaac gaaattatct ttcagcaagg
      541 agctgtgaaa atgtctcaaa ataaggcttt cagcacgcca tttatcctgg ctgttctttg
      601 tatttacttc agctatttcc tgcacggcat tagtgttatt acgcttgccc aaaatatgac
      661 atctctggca gaaaagtttt ccactgacaa cgcgggcatt gcctatttaa tttccggtat
      721 cggtttgggg cgattgatca gtattttatt cttcggtgtg atctccgata agtttggtcg
      781 tcgggcggtg atattaatgg cagtaataat gtatctgcta ttcttctttg gtattcccgc
      841 ttgcccgaat ttaactctcg cctacggtct ggcagtgtgc gtaggtatcg ctaactcagc
      901 gctggatacg ggtggctacc ccgcgctcat ggaatgcttt ccgaaagcct ctggttcggc
      961 ggtcatactg gttaaagcga tggtgtcatt tgggcaaatg ttctacccaa tgctggtgag
     1021 ctatatgctg ctcaataata tctggtacgg ctatgggctg attattccgg gtattctgtt
     1081 tgtactgatc acgctgatgc tgttgaaaag caaattcccc agccagttgg tggacgccag
     1141 cgtagctaat gaattaccgc aaatgaacag caaaccgtta gtctggctgg aaggtgtttc
     1201 atcggtactg ttcggtgtag ccgcattctc gaccttttat gtgattgtgg tgtggatgcc
     1261 caaatatgcg atggcttttg ctggtatgtc agaagctgag gcattaaaaa ccatctctta
     1321 ttacagtatg ggctcgttgg tctgtgtctt tatttttgcc gcactactga aaaaaatggt
     1381 ccggcccatc tgggctaatg tatttaactc tgcgctggca acaataacag ctgccattat
     1441 ctacctgtac ccttctccac tggtgtgtaa tgccggcgcc tttgttatcg gtttctcagc
     1501 agctggcggc attttacagc tcggtgtttc ggtcatgtca gagtttttcc ccaaaagcaa
     1561 agccaaagtc accagtattt atatgatgat gggtggactg gctaactttg ttattccgct
     1621 gattaccggt tatctgtcga acatcggcct gcaatatatc attgttctcg attttacttt
     1681 cgcgctgctg gccctgatta ccgcaattat tgtttttagc cgctattacc gcgtttttat
     1741 tattcctgag aatgatgtgc ggtttggcga acgcaaattt agcacccggt taaacacaat
     1801 taagcataga ggttaaagga gttaattatg gatgttaccg caaaatacga attgattggg
     1861 ttgatggctt atcctatccg ccacagttta tcgcctgaaa tgcagaataa agccttagaa
     1921 aaagcgggat tgccatttac ctatatggcc ttcgaagtgg ataacgatag ctttcccgga
     1981 gcaattgaag gattaaaagc cctcaaaatg cgcggaactg gtgtgtcgat gccgaacaaa
     2041 caactggcgt gtgaatatgt tgatgaatta acgccagcag ccaaactggt gggtgccatc
     2101 aacaccatcg ttaatgatga tggctatctg cgtggctata acaccgacgg cacgggccat
     2161 attcgcgcca ttaaagagag cggttttgat atcaaaggca aaacgatggt gctgttaggg
     2221 gccggaggtg cctcaacagc gattggcgcg cagggggcaa ttgaaggttt aaaagaaatt
     2281 aaactcttta accgtcggga tgagttcttc gataaagccc tcgccttcgc gcagcgggtt
     2341 aacgaaaaca ccgattgtgt cgtcacggtt accgatctcg ccgatcagca agcctttgct
     2401 gaagtcctgg cttccgccga cattttaacc aatggcacaa aagtaggtat gaaccccctt
     2461 gagaatgaat cattggttaa tgatatcagt ctgttacatc cgggactgct ggtcactgaa
     2521 tgcgtgtata acccgcatat gacgaagtta ttgcagcagg cacaacaagc aggttgcaaa
     2581 acgattgatg gatacggcat gttgttgtgg caaggggctg aacagttcac gttatggact
     2641 ggcaaagatt tccctctgga atatgttaaa caggtcatgg ggttcggtgc ctgacaggct
     2701 gaccgcgtgc agaaagggta aaaaatgaaa accgtaactg taaaagatct cgtcattggt
     2761 gcgggcgcac ctaaaatcat cgtctcgctg atggcgaaag atatcgcccg cgtgaaatcc
     2821 gaagctctcg cctatcgtga agcggacttt gatattctgg aatggcgtgt ggaccacttt
     2881 gccgacctct ccaatgtgga gtctgtcatg gcggcggcaa aaattctccg tgaaaccatg
     2941 ccagaaaaac cgctgctgtt taccttccgc agtgccaaag aaggcggcga gcaggcgatt
     3001 tccaccgagg cttatattgc tctcaatcgt gcagccatcg acagcggcct ggttgatatg
     3061 atcgatctgg agttatttac cggcgatgat caggttaaag aaaccgtcgc ctacgcccac
     3121 gcgcatgatg tgaaagttgt catgtccaac catgacttcc ataaaacgcc ggaagccgaa
     3181 gaaatcattg cccgtctgcg caaaatgcag tccttcgacg ccgatattcc taagattgcg
     3241 ctgatgccgc aaagtaccag cgatgtgctg acgttgctta ccgcgaccct ggagatgcag
     3301 gagcagtatg ccgatcgtcc aatcatcacg atgtcgatgg caaaaactgg cgtaatttct
     3361 cgtctggctg gtgaagtatt tggctcggcg gcaacttttg gtgcggtaaa aaaagcctct
     3421 gcaccagggc aaatctcggt aaat
//
LOCUS       DAEEKQ010000284         3383 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.290, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000284 DAEEKQ010000000
VERSION     DAEEKQ010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3383)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3383)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3383
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.290"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            163..408
                     /locus_tag="KLK57_004177"
     CDS             163..408
                     /locus_tag="KLK57_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894386.1"
                     /translation="MTTKHAKKPQSHALDLTEHWLRVAIKIIDRNAGEGYAKAHPELI
                     SAFMTTTAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            405..827
                     /locus_tag="KLK57_004178"
     CDS             405..827
                     /locus_tag="KLK57_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE0894387.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATIWLSVLAFGRQADALAKHQKGELVSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALNRAKQQAGNDDPYGDNIPF"
     gene            1487..3354
                     /locus_tag="KLK57_004179"
                     /pseudo
     CDS             1487..3354
                     /locus_tag="KLK57_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000860399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
BASE COUNT          985 a          757 c          911 g          730 t
ORIGIN      
        1 tttattgagc tttaagggtg aacagtggtg aacagttggt gaatagtttt tgtgaaactg
       61 ttaacccatt aacatcatga ataaaaaggg aaaattgcaa aaggtgaaca ggtgaagggt
      121 taaaatgcaa aaattttatt ttattgctgt gaggtaaagc ctgtgacaac gaagcacgcc
      181 aaaaaaccac aatctcacgc ccttgatttg acagaacact ggctgagggt ggcgataaaa
      241 atcatcgacc gcaacgccgg ggaaggatac gcgaaagcac atcccgaact gataagcgca
      301 ttcatgacca cgacggcggc aaactttgcc acgctgacag aacgggagat tgccgaagcg
      361 gaacaggtga caaccatcaa cgttaaaacc ggagagcaga cagcatgaca gcacagatag
      421 cggcttacgg gcggctggtg gctgacccgc agttaaagac caccagcaag ggtacgcaaa
      481 tgacgatggc gagtatggcg gttcccctgc cgtgcagcca ggcagatgac ggaacggcga
      541 cgatatggtt atccgtcctg gcgtttggca gacaggctga cgcactggca aagcaccaga
      601 aaggcgagct ggtgagcgtg gcgggtaaca tgcaggtaag ccagtggaca ggccagaacg
      661 gagaaacgcg gcagggctgg caggttatcg cagacagtgt aatcagtgcc agaacggcgc
      721 gaccgggcgg taaaaaaggc caacaggggc aggccactga tgcactgaac agggccaaac
      781 aacaggcggg gaatgatgat ccgtacgggg ataacatacc gttttaagca acgagtgaca
      841 gaagccggag caatccggct tttttgtagg tactcctggt gggggtggcc tgtccacggg
      901 gcgggcggtt cgcggaaaaa ggcgtatttt ttgattttta tgtcaccatc accaccagtt
      961 taagttattg atatatggca aaataaaaat ttttagtgtc gaattaggtt gttttttgtt
     1021 cgacgccgga acgttcccga aagcatttac aaaaaactgg cgcaaaaaaa agcgccccga
     1081 tggtgccacc ggaggcgctt tgtcagcgac aaaggatact gccactaccg aaatgatgag
     1141 tcttaattgt gctggcgtgg caataattag tcaattcttt tgctggtatg acaaaaatca
     1201 gaaaacacat aatctgattt ttagatagaa aatgatttat ttaccagttt tatcgatcaa
     1261 taatgatgcc cgttaatcat aacggaggca gacttatgcc agagaacaac agcagaaaac
     1321 cggataaaag tgcaacagtg cacatagacg ccggaactat ggagaagctc gaacgctatc
     1381 agcagttcat taaaagtcat caccctggaa tgccagtacc cacgaaaggg cagattgcga
     1441 gaagcgcggt tgaatactgg tacagggcaa cgttaggagc ctggctatga aaacatggtt
     1501 ttccattaag gctatggctg atgttgtaca tgtacgcatt tatgacgaga tcggcgggta
     1561 cggtgtaaaa gcatcggcac ttactgacga gatcaacgcg tgcggtaatg tgcctgaaat
     1621 ccatcttcgc atccattcac ctggtggcga catctttgaa gggctggcta tctataacgc
     1681 cctgaaaaat catccggcaa agaaaattgt acacattgaa ggcatggcgg cttctatggc
     1741 ctcgtttatt gccatgtgtg gcgatcacat cgttatgcct gaaaacgcga tgatgatgat
     1801 acatgccccc cgtggtgtta ctgccggagt gtcgggcgac gttcgccgct ttgctgacct
     1861 gatggacaag ctgggcgaca cgatggcgga aacctacgcc ggaagaacgg gcaggagcaa
     1921 acaggaaatc accgccatga tggaggcgga aacctggatg gatggcaatg agtgtaaggc
     1981 taatggcttc gcagatgagg ttatacccgc gattacagca atggccagaa ttgaatcaaa
     2041 acgaatcgga gatttttcga atatgccgga aaatattaaa agcatgatca gccagaaaac
     2101 tggcagcgga gaacaggaac gactcaacgg gatacgtgaa ttgtttggca cgttcaatgg
     2161 aagatataac gacctggcta taagttgtct tgctgattca ggctgtagcg ttgaggatgc
     2221 acgcgaacgc cttttactca ctatgggtaa agaatcgaca ccgacaaaca aaaccacccc
     2281 cgcaaatctt tactacgcgt acacggataa cggcaacata accggcgatg caatgcgtca
     2341 ggggcttaat gcgcgtctgg gacatgaaca ggcagaacgc ggcaaccctt acgccatgat
     2401 gagccttttc gatatggcac aggcatcatt aacccatcgc ggcataagca caggcagcta
     2461 cagcacacgc tcgcagatag taaacgcggc attcacccac agcagcagcg attttactta
     2521 tatccttgct ggtggtgctg aaaaatccgt gaacacagcg gcgaaacgtt ccgccagtgg
     2581 acgaaaaaag gttcattgtc aaacttccat gaggcacgtc gtgttggcct gaatggcttc
     2641 tctacattaa acaaagtgcc ggaaggcgcg gaatataaat acatcaccac cagtgaccgt
     2701 ggcgaaccaa tcgcgctggc aacttacgga aatattttca gcattacccg acaggcgatt
     2761 attaacgatg acctgaatca gttaacagtt atcccacagg caatggggcg agcagccgcc
     2821 agaacggtgg gtaatctggt tcatctggtg ctgacaggaa acacaaagct ttctgacggt
     2881 ataccgctgt tcagcaaaaa acacggaaat gatattgata acggagtcag cactacaggg
     2941 cttggcattg cacgccgtgc aatgcgcaca cagagggatg aaaacggtga ggtgctgaat
     3001 attgtgccaa aattccttct tgttcctgcg gctctggaag acagagcgtt gcaaatggtt
     3061 aactcaacca cgcctttcgg tgccgataca aacagtggtt catataaccc gtaccacaaa
     3121 caactggata ttattgtcga tccccgcctt gatgaagtca gtgataagca atggtacatg
     3181 ctttcagcac agggaacgga cacaattgag gtggcttatc ttgacggtaa tgacgagcct
     3241 taccttgagc agcaggaggg ctttattgtt gacggtgtgg cctggaaggt ccgcattgat
     3301 gctggcgttg ctgctctgga ttatcgcgga atggtcagag cgatttcttc atgagaacaa
     3361 ggcggcagca gccgcctttt ttg
//
LOCUS       DAEEKQ010000285         3382 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.291, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000285 DAEEKQ010000000
VERSION     DAEEKQ010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3382
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.291"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(8..268)
                     /gene="yfhL"
                     /locus_tag="KLK57_004180"
     CDS             complement(8..268)
                     /gene="yfhL"
                     /locus_tag="KLK57_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HBE0894388.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            483..1673
                     /locus_tag="KLK57_004181"
                     /pseudo
     CDS             483..1673
                     /locus_tag="KLK57_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1858..3045)
                     /locus_tag="KLK57_004182"
     CDS             complement(1858..3045)
                     /locus_tag="KLK57_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HBE0894389.1"
                     /translation="MLPRFADIFQQGNHWLNWLEKQPERAVRPVVIESVTKIMACGTT
                     LMGYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPDCPWQHIVF
                     TLPCQYWSLVFHNRWLLTEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHI
                     HLSTTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKR
                     EWNRFLDTHYRRGWNVNVSRVMDNAPHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLR
                     YNSHRTKREEYLLMSGDEFMERFSWHVADKGFRMVRYYGFLSPAKRRLLEEVVYIITE
                     TVRKTAMQITWRGMYQRLLKVDPLKCVLCGSQMRFTGLKRGYRLAEQVLMHEPLARMR
                     WCG"
     gene            complement(3045..>3382)
                     /locus_tag="KLK57_004183"
     CDS             complement(3045..>3382)
                     /locus_tag="KLK57_004183"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894390.1"
                     /translation="KPASQRSKLPRYVVKLHEDDFFDEEDAEVLRFDSFDDAVECCAD
                     LNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATILSGISAGGMYCPECGTVH
                     WPDGVAPPF"
BASE COUNT          846 a          918 c          798 g          820 t
ORIGIN      
        1 tagaatatta aattttatcc gcgtggtgca tcagcacaaa tttatcccac aactgttctt
       61 ctgtctcgac atgcgccgga tctttcacaa tagtattggg gatcgggcac accttctggc
      121 aggttggtgt ctcgtagtgc cctacgcatt cggtacactt atcgctgtta atctcgtaga
      181 tatgatctcc cattgaaatc gcctcattcg ggcattcggg ttcacacata tcacaattaa
      241 tacagcgttt agtaattagt aaagacattt caatggatta ccgttaaatc attttaaaat
      301 cagtaagttg tatcgagttt gtatgcttta ctgtcattaa cttactgtat gttgatccag
      361 tgtatttaac cttgataaac tcagtccagc aacacaaaac cgcaacacat tgcattttgt
      421 cccgtagaaa agacttgtat gtgtgagctt gttttctgcg cctacgcaga taaggattga
      481 gaatgccgcg cactgtaaca cataatccgg atagccccaa taatgacgat gttttagccg
      541 catctgaaaa atgggacgcc tgtaaacccc cctataccag cgcacacatg aaaatctgtg
      601 ttgctgccgc caaaatcatc ctcgctgctt ccggcgtggc tcgccgttcc aaatacgaaa
      661 aagagaacta tctccgtatc gatttcagca aagccggtaa ggttacattt tacgccgagt
      721 ttccaaaaaa gatgggcctc aaaggtaaaa agctcggcga gtggccggag ctcgctatcc
      781 agctggcgcg cgaaaaagca ctaggtatgg ctgacggtgg actgcgggca gagtccgtac
      841 atgcagcgct ggaaatgtac cgggatgacc tcaaagccaa agtagcccgg cagaagctga
      901 gcccggacag tttcacaacc tacggggtgc gtatcgaccg gattaaagca acgttcggcg
      961 agcgtgaggt gttcagcgac gtaacataca atcggctggt ggaagtgctg gacgagtgga
     1021 tcgccactcg ctcgaacaat aacgccctgg agttgtttgc cgagctccgt cggttctgga
     1081 agttctgcgc acctactctt tgcaacggcc gcaatgttgc cgccagtctg ccagatgatt
     1141 atgtttcctc ccgcgtacag aaacctaccc ccacacggct ttttaccgat attgaatcaa
     1201 tcgcccgact ctggctcaat gttgctgcct gcacctctgt acaccagaag aatgctgttc
     1261 gcttcatgat catcactggt gttcgtccga ttaatgtcca taacctgcgc tgggactacg
     1321 tttacgagga ggctggtgaa attgtttatc cggaaggggt tatcggcatg cgaggggcta
     1381 tgaaaacaca aaaggctttc cgcctgccga taacgcctga gatccggcgg attatcgacg
     1441 agcagaaagc ctggcgtgat tcagttcctg agtgcaacag ggattatgta tttttgcagc
     1501 cacgtgatcc aatgcagcca ttttcaaaac gatcactgga taagctggtg aaaacataca
     1561 gcccggacgg ggctgtaaaa ggaataaaac atgatgggac tgttaaaggg aaagacggtg
     1621 catttaatac gatgtgccgt aaattcctta agagcaatgt tattgccttg atcgatagga
     1681 attaaaaccc caaaaagatt aaaaaaacac cacaaaacgg atgtttcttc aacaccactt
     1741 ttgctcatat gaacggaacc gatgattaaa ctggatggct ctgattgatt cagggtatga
     1801 atggcggttt tttgctccgt ttccctcaaa atggacgcaa ctttccctct gcggctctca
     1861 gccgcaccac cgcatccggg ccagcggctc atgcatcagg acctgctctg ccagacggta
     1921 gccccgcttc agccccgtaa aacgcatctg actcccgcac agcacgcact tcagcgggtc
     1981 aaccttcagt aacctctgat acatccctct ccaggtgatt tgcatcgccg tttttctcac
     2041 tgtctccgtt atgatgtaca ccacttcttc cagtaaccgc cgtttcgccg gactcaaaaa
     2101 accgtagtac ctcaccatac ggaacccctt atccgccaca tgccaggaga acctttccat
     2161 gaactcatct ccactcatca acaggtattc ttcccgtttt gttcggtgac tgttgtaacg
     2221 cagaccgatt tcatcctgac cggcataatg ctccagacga ctcatcggca ccggtggctt
     2281 tttcaggtaa gagccaaagt acaccgccac atggggggca ttatccatca cccgggatac
     2341 gttgacattc cagccacggc ggtaatgcgt gtccaggaag cgattccatt cccgtttact
     2401 gcttccttct gctgccagcg catccggcat caccaggtca gggtatttcc gtgacagcaa
     2461 ccgtgttatc cggtagcgcc acatgctcat caccttacgg gcgtaaaaat gaagattttt
     2521 ccaggtgtgg cccgacgtca caccaccggc agttgtcgat aaatggatat gcggatgcca
     2581 ctgctggtca cgcccccatg tgtggatcac cgtgaatatc cccggctcca catctgcctg
     2641 atggcagatt tccagtatca catccgctgc aatgcggctc atctctgtca gtaaccaccg
     2701 gttgtggaac accagggacc agtactggca gggaagtgtg aacacaatat gctgccacgg
     2761 gcagtcgggg accaggctca gcagatactg tatccactgt gcgccagcct tcaccccgca
     2821 gtgcgggcag gagcggcttt tacaccggaa gcagaccttt tttgtatggc aacagtccgg
     2881 tgatgaacag caccactgtg tataccccat cagtgtggtc ccgcacgcca tgattttggt
     2941 caccgactca atcaccaccg gacgtactgc cctttccggc tgcttctcca gccagttaag
     3001 ccagtggttt ccctgctgaa agatatcggc aaaacgggga agcatcagaa gggcggggcg
     3061 actccgtccg gccagtgaac cgtgccacac tccgggcagt acataccgcc ggcgctgata
     3121 ccggaaagaa tggtcgcaaa ttcccgctcc gtgcagcggg cgatttccgg atacccttcg
     3181 tcatcaacac gtacaaacca gaagaccagc tttttgtttc ccgcatccac aaagaacgga
     3241 atattcaggt ctgcgcagca ttcaacggca tcgtcaaaac tatcaaagcg cagaacttct
     3301 gcgtcttctt cgtcaaaaaa atcatcttcg tgaagcttca cgacatagcg gggaagtttg
     3361 cttctttgag aggcgggttt ac
//
LOCUS       DAEEKQ010000286         3356 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.292, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000286 DAEEKQ010000000
VERSION     DAEEKQ010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3356
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.292"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1245)
                     /gene="ccp"
                     /locus_tag="KLK57_004184"
     CDS             complement(<1..1245)
                     /gene="ccp"
                     /locus_tag="KLK57_004184"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HBE0894391.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLGVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFH"
     gene            1650..3299
                     /locus_tag="KLK57_004185"
     CDS             1650..3299
                     /locus_tag="KLK57_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HBE0894392.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
BASE COUNT          850 a          862 c          810 g          834 t
ORIGIN      
        1 atggaagtac ggtgccgtta aagcaacgtt acgtaaaccg ggtactttct gacgcaattt
       61 atcacgctct tctttagtaa cattcatacg accaatatcc gccgccgtaa tttccccaaa
      121 gttaaagtct tttttcagcc ccaacggttc aaaggaacgt ccaccgagaa taataccacc
      181 atgacaagtt gcacatttat tatctttaaa taattgatag ccttttttct gttgcgccgt
      241 cagcgcattt tcatctccac gcaaccattt atcaaatggg gaatccggcg taattaatgt
      301 tttctcaaat tcagcaatgg catcagtaat attttcgcca ctgaaacctt gcggatagac
      361 gccgaggaac tgcgctttaa gctgcggatc tttttccagc ttagcaataa tttcgtccca
      421 agatttcgac gccatttcaa tcgggttcaa cggcggtcca ccagcctgat cctgcaatgt
      481 tgccgcacga ccatcccaga actgctcaac gttaaatact gagttaaata ccgtcggcgc
      541 gttaatcggc ccaactgcgc caccaacacc aatcgatgtt tttctgccat cgacgccccc
      601 cgcattcaac gcatggcaat gagcgcatga aatggtgcta tcagccgata aacggggatc
      661 gtgatacagc gcaaagccca acgccacttt ttgcgcatcg gtaggcagtt tttgcgggat
      721 gggctgcacc ggttcattgc gatgctccgg agcagtatca ttgctggcgt aatattccgc
      781 gcgctgtttt gcaatccagg ccagtatttc cgcccgctct tcatcactca ccttacccgc
      841 ccagtgaagc gcggtataac gcgttggcgg catagtttca tactgcatca cccattcaat
      901 cttattcaga tcgctttgcg aaacgggttt atcagccagc agtgccgcac gcacggcctc
      961 gaggttaaaa gatttatatc caagcttaat gtcgtaatcc atcaactgtt tcgcgccagg
     1021 aatataataa taggcgggta attctgccga aggcgtgtgg caatagtcgc atcctttttc
     1081 gcggagaaaa cctaaaacct tattattttc actgacagcg gatgcctgaa catcggcctg
     1141 tttactgcgt ttattatcgt ggtaccacac ataaccagat aaccctaaat agcaaatcgc
     1201 gacgccagcc aggccgatcg cggtaatacg tgagaccatt ttcattattc ttccctcacg
     1261 gtggtgagtt attgtgaccc agaaaatttt ccagaatgct gcaaacgtct ctgcgagaca
     1321 aagccatcat aaaaaacagt gagatacgct ttttgatagg aatcaatcaa ttacatgaat
     1381 ataaactatc aataagatag cctgtgccta ttacccagat caggaatggc atatcatgat
     1441 tgcggcgaga gcaggattga atgttctgaa aatgaaaaac aacacgaaat ccgtattttt
     1501 ataaattcca tggatattag tgcgttatat tttttgacag gttaataaac gtgatccacc
     1561 gcacgctttg tcgcccacca ggcggagcga atgactaccc ttaaagaaaa gcccgataat
     1621 tagcgacgaa tttcggaggt tggatcctta tgctcaatca gaaaattcaa aaccctaatc
     1681 cagacgaact gatgatcgaa gtcgatctct gctatgagct ggacccgtat gaattaaaac
     1741 tggatgagat gatcgaggca gaaccggaac ccgagatgat tgaagggctg cccgcctctg
     1801 atgcgctgac gcctgccgat cgctatctcg aactgttcga gcatgttcag tcggcgaaaa
     1861 ttttcccgga cagtaaaacc tttcccgact gcgcacctaa aatggacccg ctggatattt
     1921 taatccgcta ccgtaaagtg cgccgtcatc gtgattttga cttgcgcaag tttgttgaaa
     1981 atcacttctg gctgccggag gtctactcca gcgagtatgt atcggacccg caaaattccc
     2041 tgaaagagca tatcgaccag ctgtggccgg tgctaacccg cgaaccacag gatcacattc
     2101 cgtggtcttc tctactggcg ctgccgcagt catatattgt cccaggcggc cgttttagcg
     2161 aaacctacta ttgggactcc tatttcacca tgctggggct ggcggaaagt ggtcgggaag
     2221 atttgctgaa atgcatggcc gataacttcg cctggatgat cgaaaactat ggtcacatcc
     2281 ccaacggcaa ccgcacctat tatttgagcc gatcgcaacc accggttttt gcgctgatgg
     2341 tggagttgtt tgaagaagat ggtgtacgcg gtgcgcgccg ctatctcgac caccttaaaa
     2401 tggaatatgc cttctggatg gacggtgcag aatcgttgat ccctaatcag gcctatcgcc
     2461 atgttgtgcg gatgccggac ggatcgctgc tcaaccgtta ctgggacgat cgcgacacgc
     2521 cgcgtgacga atcctggctt gaggacgttg aaaccgcgaa acattctggt cgcccgccca
     2581 acgaggtgta ccgcgattta cgcgcggggg cggcctccgg ttgggattac tcttcccgtt
     2641 ggctgcgtga tactggtcgt ctggcgagca ttcgtaccac ccagttcatc cccatcgatc
     2701 tgaatgcctt cctgtttaaa ctggagagcg ccatcgccaa catctcggcg ctgaaaggcg
     2761 agaaagagac agaagcgctg ttccgccaga aggccagtgc ccgtcgcgat gcggtaaacc
     2821 gttacctctg ggatgatgaa aacggcatct accgcgatta cgactggcga cgcgaacaac
     2881 tggcgctgtt ttccgctgcc gccattgtgc cgctctatgt cggcatggcg aaccatgaac
     2941 aggccgatcg tctggcaaac gccgtacgca gccggttact gacacctggc gggattctgg
     3001 caagcgagta cgaaaccggt gaacagtggg ataaacccaa cggctgggca ccgttacaat
     3061 ggatggcaat tcagggattt aaaatgtatg gcgatgacct tctgggtgat gaaatcgcgc
     3121 gcagctggct gaaaacggtg aatcagttct atctggaaca gcacaaaatg atcgagaaat
     3181 accatattgc cgatggtgtt ccccgcgaag gcggcggtgg cgagtatccg ttgcaggatg
     3241 ggtttggctg gactaacggt gtggtacgcc gtttaattgg tttgtacggc gaaccataat
     3301 atttttacag ccagccgcta acttcctgct ggctgtaaaa ttatcctctt caggag
//
LOCUS       DAEEKQ010000287         3331 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.293, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000287 DAEEKQ010000000
VERSION     DAEEKQ010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.293"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            271..528
                     /locus_tag="KLK57_004186"
     CDS             271..528
                     /locus_tag="KLK57_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894393.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEVLR"
     gene            528..902
                     /locus_tag="KLK57_004187"
     CDS             528..902
                     /locus_tag="KLK57_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HBE0894394.1"
                     /translation="MRHEFILPYPPTVNTYWRRRDNTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMHEEQVKK"
     gene            899..1753
                     /locus_tag="KLK57_004188"
     CDS             899..1753
                     /locus_tag="KLK57_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBE0894395.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTRLIF
                     PESVVNSS"
     gene            1856..1931
                     /locus_tag="KLK57_004189"
     tRNA            1856..1931
                     /locus_tag="KLK57_004189"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1889..1891,aa:Ile,seq:cat)
     gene            2032..2108
                     /locus_tag="KLK57_004190"
     tRNA            2032..2108
                     /locus_tag="KLK57_004190"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2066..2068,aa:Arg,seq:tct)
     gene            2608..2739
                     /locus_tag="KLK57_004191"
     CDS             2608..2739
                     /locus_tag="KLK57_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HBE0894396.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            complement(3020..>3331)
                     /gene="iraM"
                     /locus_tag="KLK57_004192"
     CDS             complement(3020..>3331)
                     /gene="iraM"
                     /locus_tag="KLK57_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HBE0894397.1"
                     /translation="VIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFTLTHTGIML
                     NNKVLPVTIYNVVPFNKTFWNSIKNSQECPTNTDNVLNECFNNRCTLQICPYGLKQQS
                     P"
BASE COUNT          874 a          656 c          896 g          905 t
ORIGIN      
        1 gcgatctttt cctgtgtggt acgccgtctg cgtttctccg gccctaagac atcaatcatc
       61 tgtactccaa tgactagtct aaaaactagt attaagacta tcacttattt aagtgatact
      121 ggttgtctgg agattcaggg ggccagtcta cggacatttt ctgcctgtgg accaggaaag
      181 tgcacgggtg ctttccactc ccatgaagcc cggcaatccg cgcagataca gtaataaacg
      241 aactgacagc agtaagatgg agcgtttctt ttgaacaacc agacaatgac ttttacccct
      301 gaacaattgc gtaaacacgc gcaggaaatg ctgcgtcatg ctgaacaact cgaaaaaacg
      361 ggcataacaa aagacgctat ccgtaaagat atggtacccg cgcttcggga actgatgcag
      421 gcgaagcatc gcgcacagaa agcagtagat gagctggtgg actgtgtggc agagctggaa
      481 accagagtcg gaaagtttga aaaactggtg caggaggtgc tgcgctgatg cgtcatgagt
      541 ttattttacc ttatccgccg acggtgaata cttactggcg acgtcgtgac aacacatatt
      601 ttgtatcaaa agccggtgag cgttatcgcc gggatgtggc gcttattgtc cgtcagcagc
      661 gactgaaatt aagcctgtcc ggaagactgg cgataaagat tattgcagag ccaccggata
      721 agcgccgtcg tgacctggac aatatcctga aagcaccact ggatgcactg acacatgcgg
      781 gagtgttaat ggacgatgag cagtttgatg aaatcaatat cgttcgtggt cagccagtat
      841 ctggtggacg tctgggggtg aagatttacc ccataatgca tgaagagcag gtcaaaaaat
      901 gaaactggaa gatttaccga aatactactc cccaaaatcc ccttgcctga ccgatgcatc
      961 ggcctcaacg tcaaaagatg cgctgagtat cactgatgtg atggccgcgc agggcatgac
     1021 acagaatcgg gctgagatgg ggttttctgc gttcctgggg aaaatgggca tcagtatgaa
     1081 tgacagggcg cgggcaacag aattactggc agattatgca ctcagtcggt gcgatcgtgt
     1141 ggcggcgttg agaaaacttc cggcagaaat aaaaccggta gtgatgcgca ttatggcttc
     1201 gtacgctttt gaggattatg cccgcagcgc agcgagtaaa aagcagtgcc cttgttgcta
     1261 tggggaaaaa tttattgaaa gcgtagtttt tacaaacaag gtccagtatc cggatggtaa
     1321 gccgccggta tgggcaaagt gtacgaaagg tgtgtatccg tcttactggg aagaatggaa
     1381 aaaagtcagg gaggtggtaa aagttgcctg tccggagtgt ggcggaaagg gtgaggtttc
     1441 caccgcctgt aaggattgcc gtgggcgtgg tgtcgccatt catcgtgaag agtcggtaaa
     1501 acgtggtatg cctgttatca gagactgcca gcgttgtggt ggtcgtggct atgaaagact
     1561 accatcaacg gaggcattta atgctatatg cgaggtgaca aaccagataa cacgcgcgtc
     1621 atgggaaaaa acagttaaga aattctatga tgcgctggtg actcggtttg atattgaaga
     1681 agcatgggct gagcggcagt taaaaaaggt aactaggttg atttttccgg aatctgtggt
     1741 aaattcgtca taactatggg cgttttatgc ctgacgttag aagagtttct acaacccgcc
     1801 gccgagcggg ttttttattg cggaattaat tacggaccgt tattattctg ctcccggccc
     1861 tttagctcag tggtgagagc gagcgactca taatcgccag gtcgctggtt caaatccagc
     1921 aagggccacc atcacaaacc gccattagct tatcaggaag agcagacgac acgataacag
     1981 ggttgttggt gcgggggcgg gtccccgatg gcggtccatt atcggtattc agcgttgtta
     2041 gctcagccgg acagagcaat tgccttctaa gcaatcggtc actggttcga atccagtaca
     2101 gcgcgccata ttcattcttc cagattcctt ccggcagagc cttatactga aatatacctg
     2161 gctcaggata ttgttgaaaa tattttatgt ttgtcaaaaa taaaagttct gttaagtatt
     2221 gattgagtgt ttgttatacg gtctaatggt tttttcagca ttaaatattt atcattcata
     2281 tggtgtgggt agagtgaata ttgatgaggc gtcggggtgt ttcatcctta ggcagcgtat
     2341 tgatatagtc aatgcagcac gagcaaaggc cttcagccgt ttgacagttt tgttctgtac
     2401 tcctgatcgt ctttcgggaa gagacgttat tattctgaat agtgatgcta tacagagggt
     2461 ttgcgatgag ttcatggttg ctaattcaga attatttgct cttgttcagg agtacaacag
     2521 aatagccagg acctgtggta tggatgaact tcggattact catctggggt agatacatat
     2581 ctggattatc acctgttacg gtaaaaagtg attgcttact gtttttgtga atggcattgc
     2641 agcagccgga taatgtcagt gctggctgac ggtgtgctgg tggcgggtgt ggtggttgtt
     2701 gctttcccgt tgctgaaaaa gaaaacgcca gactgttagc cgggtatcag ttagcgggag
     2761 aaatttttaa atacttcaca attcaggcgg ttgactgttg tctggtttgc ggggagtttg
     2821 ttaaaagaaa ctggcatggt gaatccccct gtgcggaggg gcaatcagcg agtaggtata
     2881 tgggataatc gcggattcag gtgctggtac tgaattcacc gggaggcacc cggcaccatg
     2941 caatggcaca tagcgccact ctccagcccc tctccggagg ggctgtttat attgattttg
     3001 tcagatgtga gtaaactcct tatggacttt gttgttttag tccataagga catatttgca
     3061 gagtgcaacg gttattaaag cattcattca atacgttatc tgtatttgta gggcattcct
     3121 ggctattttt gattgaattc cagaatgttt tattgaatgg tactacgttg taaatggtta
     3181 caggtagcac tttgttattg agcatgatgc ctgtgtgagt cagtgtaaat atactttcag
     3241 gaggtaagaa agcatccgat tgataccaga ttattaattt tattttactc catatgactg
     3301 aaaaagatat tccgcatgat ggctggataa c
//
LOCUS       DAEEKQ010000288         3323 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.294, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000288 DAEEKQ010000000
VERSION     DAEEKQ010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.294"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..843)
                     /gene="adrA"
                     /locus_tag="KLK57_004193"
                     /gene_synonym="dgcC"
     CDS             complement(<1..843)
                     /gene="adrA"
                     /locus_tag="KLK57_004193"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HBE0894398.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRG"
     gene            complement(945..1265)
                     /gene="psiF"
                     /locus_tag="KLK57_004194"
     CDS             complement(945..1265)
                     /gene="psiF"
                     /locus_tag="KLK57_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HBE0894399.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="KLK57_004195"
     CDS             complement(1384..2799)
                     /gene="phoA"
                     /locus_tag="KLK57_004195"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HBE0894400.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTSDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKTIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="KLK57_004196"
     CDS             complement(2900..3160)
                     /gene="iraP"
                     /locus_tag="KLK57_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HBE0894401.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT          789 a          879 c          783 g          872 t
ORIGIN      
        1 accgcgcagg gtaatttgta actgtcgggt aagcgccaca atcgcttcat cgcccacatc
       61 atgaccccag gtatcgttga tgctcttgaa atggtcgata tcgataatca gtaacgttgc
      121 atcgcgatta tgccgccgac agttatcaaa ttcattgcgt aacatagttt cccaatgacg
      181 tcggttatac acgcccgtca tgccgtcgcg agtactcatg acctgcaacc tgcgtttatg
      241 ttccgccagt ttggttgccg tctggtagct gacccagcca aacagcagag gataaacgac
      301 aataatggga agggagagcc accattccag cggcgcacta ttgaacgaca cggtaatgcc
      361 cgtaagctcg agggtgacaa ggcaggaaac caccatcaac accagacccg cgacaaacag
      421 acgggggccg cctgccccca tcaaattcag acacataatc atcaacatag cggtggaagg
      481 cagcacgttt acgcccatta cgcctaccca cattcccgct aatactgcat cggtttttaa
      541 gttgtaaatt tcccggctaa gcggatcgac ggccctgctc gctatctgcc aggctaaatg
      601 cggccagacg aacgcccagc cgaccaacac cagccaccac cagcccggcg gcgggtgcga
      661 aaccagcgtt gaagcaatcg gtaagaacat gccagccagg ccaaccgcac ggggtagtct
      721 gacgcgacgg gcgaagcgca gcccggaccg ctgatgttcg ttttgaggag ttaaaggagg
      781 ttcctcccca tgcgccgccg cttttttgaa aaagttttca tcattcatta tttttgggaa
      841 cattcagaaa caattttccc aaattataga gacggggcta aaggcagaag tatgaaattt
      901 cgggtgagcc actttcgtag ctcacccgtt agcactatca ggtatcaggc ggctttcttg
      961 aggcaggcac tcataaactt attacgatca tcacctttca gagattgttg cgttgcttga
     1021 ttattacatt cgcgcatctt ttgctgctgt ggcgtcaaac ttttttcacc aggcgcagac
     1081 ttgctgttct tcaggcaatc actcatgtag gtcttacgag catccccttt caacgcctgc
     1141 gccgtcgcct gctgattaca ggaggtcata cgctgttgtt gtggggttaa ggttctctcg
     1201 gcagcgccga cggtggttaa aaaaaccaga ccaaaaagca aggtaaccag taatgttatt
     1261 ttcatagcac catccctctt catgttttaa ccatgagcgt ttgcgcccgt gatctgccat
     1321 taagtctggt tgctaacagc aaaaaaacca cccggcagcg aaaattcact gccgggcgcg
     1381 gttttatttc agccccagag cggctttcat ggtgtagaag agatcggtct ggtcggtcag
     1441 tccaacaaca ttggcggcat gcggaccata cgccgcaata cgcaactgac tgccggtatg
     1501 ttcttgtgaa tcctcttcgg aattcccgta actcatcacc atcactgcgc catctttagt
     1561 atttagcgcc tgagtgaggc ccggcgcttt ggtatccggc gcaacaacct ggctggcgtg
     1621 ggcgtgatca gcggtgacta tgaccagcgt gttaccctcc tttttagcga attccagcgc
     1681 ccgttgtacg gcttcatcga gatcgaccgt ctcgccaatt tgcccacaag gattcgcagc
     1741 gtgatcctgt ttatcgattg acgcaccttc aacttgcagg aaaaatcctt tctcattttt
     1801 actcaacaat tcaatggttt tgtcggtcat ctgtgccagg gttggtacac tgtcattacg
     1861 ttgcggatta ggcgtacagg tgactgcggg cttatcgata ttgccgtggt acgttgcttt
     1921 cggccctagc cagcgcactg gcatattgcc gtcagcaaac aggccaagca ggggtttttg
     1981 ctgattcgct tccgtcaccg aattcagtga ggcagcatcg ttcaccaact gataaccacg
     2041 cgcctgtgcc tgttcacgca gcgtttttcc ctgccattca ccagcggttg ccgtttcagc
     2101 aaaggttttt gcgccgccgc caagcgtaac gtcggcacga gcgttaagca gctgttcggt
     2161 aatcgatcct tttccgcctt tttccagagc gttacccgga catttttcac tggtcgcgct
     2221 cggaccgtag catttgcgcg aagtcacatg tgccaccagc gcagcgggcg tggcatcctg
     2281 caactctgcg gtagaaacgt taccggtcgc cagacctgcg gcttttgcca tttccagaat
     2341 cgttgggtga tctttttcgt gaatatcgac gcccagcgcg ccgttatagg ttttgacacc
     2401 ggttgaccag gcggttgctg atgcagccga gtcggtgacg tagtccggtt tgccagtttt
     2461 tttattcagc gcatagtgag tgtattgccc ggtaagcggt aaggcatcga tacctttaaa
     2521 aaagccgccc gcaccttcgg cataattacg tgcggcagta atttccgaat cccccatccc
     2581 atcgccaatc agcaaaataa tattttttgc aggtttatcg ctaagagaat cacgcagagc
     2641 ggcggtctga tcactcgtta agcggcgagc accgccgggt gcagtaatat cgccctgagc
     2701 agcccggttt tccagaacag gcatttctgg tgtccgggct tttgtcacag gggtaaacag
     2761 taacggtaag agtgccagtg caatagtgct ttgtttcact ttattttctc catgtacaaa
     2821 tacattaaaa aataaaaaca aagcgactat aagtctcggc cgtgacaact ttatgacagc
     2881 tgttgaaaag attaactttt tactgacgag gatgcttcaa taacttcttt acgtaatcgc
     2941 gcagcagctc cgtatcgtcg tcaggaatgc tggcatcggg ctttacctcg tacagcgccc
     3001 cctctacctg atcaatcaac cgctgttggt cattttgcgc catattgcga agcattgcag
     3061 tgacgataat ctccaaagct tctacctgcg cacacagttc tttcgactct tcttcttttt
     3121 gggcaagctt aaataacaac tcagcaatga gatttttcat gtctgtattt ccttatccaa
     3181 agtatggaga agttaacatt atgtttcaca acttcatagt gtctggaagt attattttta
     3241 tgacaaagga aatactttgc gcaaatattt tttgtttgat taccagcgaa acgtttctct
     3301 tatttaaata attacccaca caa
//
LOCUS       DAEEKQ010000289         3318 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.295, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000289 DAEEKQ010000000
VERSION     DAEEKQ010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3318
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.295"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="KLK57_004197"
     CDS             complement(<1..321)
                     /gene="fliZ"
                     /locus_tag="KLK57_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HBE0894402.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDMLLDENGWQVEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTV"
     gene            complement(367..1086)
                     /gene="fliA"
                     /locus_tag="KLK57_004198"
     CDS             complement(367..1086)
                     /gene="fliA"
                     /locus_tag="KLK57_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HBE0894403.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDSNLRQRVMEAIETLPEREKLVLTLYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(1251..2825)
                     /locus_tag="KLK57_004199"
     CDS             complement(1251..2825)
                     /locus_tag="KLK57_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HBE0894404.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALSEINNNLQRIRELSVQATNG
                     TNSDSDLNSIQDEITQRLSEIDRVSNQTQFNGVKVLASDQTMKIQVGANDGETIEIAL
                     DKIDAKTLGLDNFSVALGKVPMSSAVALKSEAAPDLTKVNATDGSVGGAKAFGSNYKN
                     ADVETYFGTGNVQDTKDTTDATGTAGTKVYQVQVEGQTYFVGQDNNTNTNGFTLLKQN
                     STGYEKVQVGGKDVQLANFGGRVTAFVEDNGSATSVDLAAGKMGKALAYNDAPMSVYF
                     GGKNLDVHQVQDTQGNPVPNSFAAKTSDGTYIAVNVDAATGNTSVITDPNGKAVEWAV
                     KNDGSAQAIMREDDKVYTANITNKTATKGAELSASDLKALATTNPLSKLDEALAKVDK
                     LRSSLGAVQNRFDSAITNLGNTVNDLSSARSRIEDADYATEVSNMSRAQILQQAGTSV
                     LAQANQTTQNVLSLLR"
     gene            3080..3318
                     /locus_tag="KLK57_004200"
                     /pseudo
     CDS             3080..3318
                     /locus_tag="KLK57_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460913.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
BASE COUNT          849 a          790 c          711 g          968 t
ORIGIN      
        1 caccgtacct gggctcattt cagtttgctc aaaaagaaat tgcttaaagc cgataatatc
       61 gaaaaaatca ctctgcgttt tgcagtgtaa atcaccacaa aatcggcaca atgccgccca
      121 tgatttttgt tccacttgcc agccattttc atcaagcagc atgtccaggc gggaaatctc
      181 gattttattc actattttgc cgtcgcgaac gagggtaatg cggtcgagta atttacggca
      241 atgcgcgcaa tgagtctggc tgtgtttaaa gtctttaaga tagcggctta atggccgtct
      301 tttcaggtgc tgcaccatca ttaagaactc ctggtagtca aaattaaagt gcggcattta
      361 ctgacgttat aacttaccca gtttagtgcg taaccgttta atggcctggc tgtgtaactg
      421 actgacccgc gattccccga cctccagcac cgcgccaatc tctttgagat tcagctcttc
      481 ctggtaatag agggttaata ccagtttttc gcgctccggc aacgtttcga tggcttccat
      541 cacccgctgg cgcagattac tgtccagcag ttgttgtagc gggttttctc gctgatgatc
      601 atcagtaacc agttcgatgc tatcgccgtg ctcttcgcgc cactcatcgt aggagaagag
      661 ctggctgtta ttggtgtcga gcaacatttg gcgataatcg gcaatatcga tccctaaacg
      721 ttccgctacc tcagtttccg tggcgttgcg gccaagttcc tgctccagtt gccctattgc
      781 ctgtgccact tcacgcgcgt tgcgtcgcac gctgcgcggc acccagtcac ggctgcgaag
      841 ttcatccagc atagcgccac ggatacgctg cactgcgtaa gttgtaaatg ccgttccttg
      901 tagggcgtca tagcgttcga cggcattaag taacccaatg ccgcccgcct gtagcagatc
      961 gtcaagttcc acgctcgcgg gcagtcgaac ctgcaggcgc aatgcttcgt gacgcaccag
     1021 cgggacataa cgctgccaca gcgagtgttt atccattaca ccttcagcgg tatagagtga
     1081 attcacgata aacagccctg cgttatatga gttatcggca tgattatccg tttctgcagg
     1141 ttttttaatc ggacgattag tgggtgaaat gaggggttat ttaggggtta ccggtaaatt
     1201 gcggacagaa aaaaccccgc cggtggcggg gtttgagcga taagtgtaaa ttaacgcagc
     1261 agagacagta cgttctgcgt ggtctggtta gcctgtgcca gaacagaggt acccgcttgt
     1321 tgcaggatct gcgcacgaga catgttagac acttcggttg cgtagtcagc atcttctata
     1381 cggctacggg cagaagacag gtcgtttacg gtgttgccaa ggttggtgat ggcagagtcg
     1441 aaacggtttt gtactgcacc caaagaactg cgcaacttat caacttttgc caaagcttcg
     1501 tctaatttgg ataatggatt tgtggttgct aaggctttca aatctgaggc actcagttca
     1561 gcacctttgg ttgccgtctt attcgtgata ttggctgtat aaaccttatc atcttcacgc
     1621 ataattgcct gtgcagaacc atcatttttt actgcccatt caactgcctt accattagga
     1681 tcagtaataa cagacgtgtt acctgtagcg gcatctacat ttactgcaat gtaggtgccg
     1741 tctgatgttt tagcagcaaa tgaattaggt acaggattcc cttgggtatc ttgtacttgg
     1801 tggacatcta ggttttttcc cccaaaataa acagacattg gtgcatcatt ataagctaat
     1861 gctttaccca ttttacccgc agctaaatca actgatgtgg cagaaccatt atcttcaaca
     1921 aatgcagtta cacgaccacc aaagtttgct aactgaacat ccttaccacc cacctgaact
     1981 ttttcataac ctgtagagtt ttgtttcaat aatgtaaaac cgttcgtgtt ggtattatta
     2041 tcttgaccaa caaaataagt ctgcccttcc acctgtactt gataaacttt tgttcctgca
     2101 gtaccggtcg catcagttgt atcctttgta tcttgtacat taccggtacc aaaataagtt
     2161 tcaacatcag catttttata attgctaccg aatgctttag cacctcccac actaccatca
     2221 gttgcattta ccttagttaa gtcaggagcg gcttcgctct taagtgcaac cgcagaggac
     2281 attggaactt ttcctagtgc tacgctaaag ttatcaagcc ccaaggtttt agcatcaatt
     2341 ttatcaaggg caatctcaat ggtttcacca tcgttcgcac ctacttgaat tttcatagtc
     2401 tgatcagaag ccagaacttt tacaccatta aattgtgtct ggttagaaac acgatcaatt
     2461 tcactaaggc gttgtgtaat ttcatcctga attgaattca ggtcggagtc agagtttgta
     2521 ccattagtgg cctgtactga caattcacga atacgctgta agttattgtt gatttcagaa
     2581 agcgcacctt cagtggtctg cgcaacagaa ataccatcat tcgcgttacg ggaagcctga
     2641 gtcagacctt taatatttgc tgtaaaacgg ttagcaatcg cctgacctgc tgcgtcatct
     2701 ttagcactgt taatacgcag gccagaagag agacgttcaa tggcggagct cagagaagac
     2761 tgagatttgt tcaggttatt ctgggtcaac agcgacaggc tgtttgtatt aatgacttgt
     2821 gccatgatct tttccttatc aattacaact tgatgttatt gggctgttgc ccacggtttc
     2881 tcaccgtacc ccttgtatcg gcacctgaat ttcgaacttt agaaaatttt agtttctctc
     2941 ctgttttttc ttaacggctg aaatatccgt aatatacaac tctactctgc gcatcatctt
     3001 cgtgaatcaa ctattaaact ttgcgcacaa attgccgata acccgcttat ctactgtttt
     3061 caatcaaaag gaattaggta tggcttcatt tacatctctt ggcgttggtt cgaatctgcc
     3121 gctggataca ttgctaaaca acctgaccat cgctgaaaaa aaacgcttaa acccaattac
     3181 tcagcaacag agtgacaata ccgcacgtct gacagcctat ggcacattaa aaatgtgcat
     3241 tagaaaaatt ccagactgca aatactgcgt taaataaagc cgatctgttc aaaagcacta
     3301 ccgttaccag tagcaatg
//
LOCUS       DAEEKQ010000290         3291 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.296, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000290 DAEEKQ010000000
VERSION     DAEEKQ010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.296"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..95
                     /locus_tag="KLK57_004201"
     CDS             <1..95
                     /locus_tag="KLK57_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0894405.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            244..1491
                     /locus_tag="KLK57_004202"
     CDS             244..1491
                     /locus_tag="KLK57_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HBE0894406.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLILGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMCFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(2020..2220)
                     /gene="torI"
                     /locus_tag="KLK57_004203"
     CDS             complement(2020..2220)
                     /gene="torI"
                     /locus_tag="KLK57_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HBE0894407.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(2343..2687)
                     /locus_tag="KLK57_004204"
     CDS             complement(2343..2687)
                     /locus_tag="KLK57_004204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894408.1"
                     /translation="MSDLAMKVLKWQSTGDVGISSATLASIACGLKKNIYGHHFGALH
                     DAADFRRCVALVEQIPEIRDSFDKVAKRVPAFKGILNEWDSLVALLKSEMKTYGNKAP
                     ETYRRISELRKD"
     gene            2929..>3291
                     /locus_tag="KLK57_004205"
     CDS             2929..>3291
                     /locus_tag="KLK57_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894409.1"
                     /translation="MSYFLCKKWMINLSGAGKILWALNMKKDSYPYLICMTVSGLIFI
                     FLFFWWRADIYRVTFLNQSISHYYILFSMGIAFLLSLFWVKKGIVKQSGWKSLSAYLK
                     VYAGICIFSGFFLIIPLTT"
BASE COUNT          813 a          679 c          764 g         1035 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc cgcttgcagt
      121 aacggcgaaa ggggcaatga cagcgctgcc atgtcgacaa gaactggaat agtgagaagt
      181 aaacggcgaa gtcgctctta tctctaaata ggacgtgaat tttttaacga caggtaggta
      241 attatggcac tgaatattcc attcagaaat gcgtactatc gttttgcatc cagttactca
      301 tttctctttt ttatttcctg gtcgctgtgg tggtcgttat acgctatttg gctgaaagga
      361 catctagggt tgacagggac ggaattaggt acactttatt cggtcaacca gtttaccagc
      421 attctattta tgatgttcta cggcatcgtt caggataaac tcggtctgaa gaaaccgctc
      481 atctggtgta tgagtttcat cctggtcttg accggaccgt ttatgattta cgtttatgaa
      541 ccgttactgc aaagcaattt ttctgtaggt ctaattctgg gagcgctctt ttttggcctg
      601 gggtatctgg cgggatgcgg tttgcttgac agcttcaccg aaaaaatggc gcgaaatttt
      661 catttcgaat atggaacagc gcgcgcctgg ggatcttttg gctatgctat tggcgcgttc
      721 tttgccggca tattttttag tatcagtccc catatcaact tctggttggt ctcgctattt
      781 ggcgctgtat ttatgatgat caacatgtgt tttaaagata aggatcacca gtgcgtagcg
      841 gcggatgcgg gaggggtaaa aaaagaggat tttatcgcag ttttcaagga tcgaaacttc
      901 tgggttttcg tcatatttat tgtggggacg tggtctttct ataacatttt tgatcaacaa
      961 ctttttcctg tcttttatgc aggtttattc gaatcacacg atgtaggaac gcgcctgtat
     1021 ggttatctca actcattcca ggtggtactc gaagcgctat gcatggcgat tattcctttc
     1081 tttgtgaatc gggtagggcc aaaaaatgca ttacttatcg gtgttgtgat tatggcgttg
     1141 cgtatccttt cctgcgcgct gttcgttaac ccctggatta tttcattagt gaagctgtta
     1201 catgccattg aggttccact ttgtgtcata tccgtcttca aatacagcgt ggcaaatttt
     1261 gataagcgcc tgtcgtcgac gatctttctg attggttttc aaattgccag ttcgcttggg
     1321 attgtgctgc tttcaacgcc gactgggata ctctttgacc acgcaggcta ccagacagtt
     1381 ttcttcgcaa tttcgggtat tgtctgccta atgttgctat ttggcatttt cttcctgagt
     1441 aaaaaacgcg agcaaatagt tatggaaacg cctgtacctt cagcaatata gacgtaaact
     1501 tttccggttg ttgtcgatat ctccctatcc ctcaaccgga aaataataat actaaagtgc
     1561 ttagcccggc taataatcac ctaatccaaa cgcctcattc atgttctggt acagtcgctc
     1621 aaatgtactt cagatgcgcg gttcgctgat ttccaggaca ttgtcgtcat tcagcgacct
     1681 gtcccgtgta tcacggtcct gcgaattcat caaggaatgc attgcggagt gaagtatcga
     1741 gtcacgccat atttcgctat caggattctg tgtgatggtt acatcgcccg gcccagggct
     1801 gtttagtcat cagcgctttc tgacagtgct gagatttcaa cctgttgcag taaaaatgag
     1861 tagatataag gcaagtgtgc tgccaaaccc atcttttacg gggtgaaggt agatttcgtt
     1921 tgaagggtat ctggtgtccc ctgcagacat ctacttgagg cggcagggga ttgattggaa
     1981 tgctgttttt tagatttgaa aaatatttta cccgctattt tacccattgg cgcggcttaa
     2041 gagcttattt ttgaattcac aatggtcacg atataaccat cttgctcgtc cgtggataac
     2101 tttggcttta ggcaggtcgc cggacttaat ccggtcatag atgaaggtct taccgaagcc
     2161 agtatcagcc atgatgaatt tcaaatcaac cagtgaatca ggttgtagtt cgtgttgcat
     2221 gagtgctatc tccgaatatg gaatcgaacc tgcaaatcag gcaataaaaa gccgcattga
     2281 tgcagcaatg gtaggtctgg atatcttgag aaatgaacag gcctcatcga gtgtgaggcg
     2341 ggttagtcct tgcgtagctc gctgattctt ctgtaagtct ctggtgcttt gttcccgtac
     2401 gtcttcattt cagacttcaa cagagcaacg agtgaatccc attcgttgag gattcctttg
     2461 aatgccggaa cgcgctttgc aaccttgtcg aatgaatctc tgatttctgg aatctgctca
     2521 acaagtgcaa cgcatcgccg aaagtctgct gcgtcatgga gagcgccgaa gtgatgacca
     2581 tagatattct ttttcaggcc acatgcgatt gaggcaagag ttgcgctact gatgccgaca
     2641 tcgccagttg attgccattt caaaaccttc atagccaaat ctgacatttc ttgtctccat
     2701 aaaacaaaac tcgccgtagc gagctcagat aaaagaaatc cccgtcagtg cgaggatgct
     2761 gttcattgct gctatacact tttttgctct caacgtaagc ggtagcccat tctgttgggt
     2821 tggtcagttg cttttaggaa atgctcttta ccccttaaac gtcggctgaa agagctaaaa
     2881 tccatgcaaa aaatttacgc aattttgtgt attattgtgc agtaagtaat gagctatttt
     2941 ctgtgcaaaa aatggatgat aaatttgtcc ggggcaggaa aaattttatg ggcgctaaac
     3001 atgaaaaaag attcgtatcc ttatttgatt tgcatgacag tttcagggct gatctttatt
     3061 ttccttttct tctggtggcg ggcagatatc tacagggtca cgtttcttaa tcagagtata
     3121 tcccactatt acattctgtt tagcatggga atagcttttc tgttatctct gttttgggtt
     3181 aagaagggga tagtaaaaca aagcggctgg aagagtctgt cagcatacct taaggtttat
     3241 gcagggatat gcatattttc tggatttttt ctgattatac cccttacaac a
//
LOCUS       DAEEKQ010000291         3222 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.297, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000291 DAEEKQ010000000
VERSION     DAEEKQ010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3222
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.297"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            108..824
                     /gene="nanC"
                     /locus_tag="KLK57_004206"
     CDS             108..824
                     /gene="nanC"
                     /locus_tag="KLK57_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HBE0894410.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            844..1950
                     /gene="nanM"
                     /locus_tag="KLK57_004207"
     CDS             844..1950
                     /gene="nanM"
                     /locus_tag="KLK57_004207"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HBE0894411.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSLAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2015..2995
                     /locus_tag="KLK57_004208"
     CDS             2015..2995
                     /locus_tag="KLK57_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HBE0894412.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPNNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTCDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT          948 a          684 c          759 g          831 t
ORIGIN      
        1 gttgccaaag acactataag cctggcaaaa aatattacac aacataaatg ctaattgttt
       61 atgcgggctt tgtattgctt tctgtatcct acaaatgagt gaaatttatg aaaaaggcta
      121 aaatactttc tggcgtatta ttactgtgct tttcgtcacc attaatttct caggctgcga
      181 cactggacgt acgtggtgga tatcgtagcg gaagccatgc ctatgagact cgactcaaag
      241 tcagtgaggg atggcaaaat ggatggtggg caagcatgga aagtaatacc tggaatacca
      301 ttcatgataa taaaaaggaa aatgccgcac tcaatgatgt tcaggttgaa gttaattacg
      361 cgattaaact tgatgatcaa tggacggtgc gcccgggaat gttaacgcat tttagcagca
      421 acggcacacg ctacggaccc tacgtaaaac tgtcctggga cgcgacaaaa gatcttaatt
      481 ttggcattcg ctatcgttac gactggaaag cttaccgaca acaagactta tccggtgata
      541 tgtctcgtga taacgttcat cgttgggatg gatatgtcac ttaccatatt aatagtgatt
      601 tcaccttcgc atggcaaacg acgctataca gcaaacagaa tgattatcgc tatgcaaacc
      661 acaagaaatg ggcgacggaa aatgcctttg ttctacaata ccatatgacg cccgatatta
      721 cgccatacat agaatatgac taccttgacc gccagggtgt ttacaacggc agagataatt
      781 tatcggaaaa cagttatcgc attggtgtgt catttaaact gtagtagaca ggagacagtc
      841 acaatgaata aaactataat ggcgcttgct atcatgatgg cttcatttgc cgcaaacgcg
      901 tctgtattac cggaaactcc tgtgccattt aaaagtggta ccggagcaat tgataacgac
      961 actgtctaca ttggtttagg tagcgcaggt acagcatggt acaagctaga tacacaggcc
     1021 aaagataaaa aatggactgc gttagctgca ttccctggtg gaccgagaga tcaagcaacc
     1081 tctgcattta ttgatggcaa tctatatgtg tttggcggca ttggcaaaaa cagcgaggga
     1141 ttgactcagg tatttaatga cgtacacaaa tacaacccca aaaccaatag ctgggttaaa
     1201 ttgatgtcgc tcgcgccgat gggcatggcg ggtcatgtga cttttgtaca caacggcaag
     1261 gcttatgtta ctggcggtgt taaccagaat atcttcaatg gctattttga agatctcaac
     1321 gaggctggaa aagattcaac cgctatagat aaaatcaacg cccactattt tgacaaaaaa
     1381 gcagaagatt atttcttcaa taagtttctg ttgtcttttg atccctcaac acagcaatgg
     1441 agttacgctg gcgaatcgcc ctggtacgga acggctggtg cggcggttgt gaataaaggt
     1501 gataaaacct ggcttattaa tggcgaagcc aaaccaggat tgcgaacgga tgccgtattt
     1561 gaacttgatt tcaccggtaa taatttaaaa tggaataagc ttgatcccgt ctcatcacca
     1621 gatggcgtcg ctggcggttt tgcggggata agcaatgatt ctcttatatt tgccggaggg
     1681 gccggattca aaggttcacg agaaaattac cagaacggta agaactatgc gcatgaaggc
     1741 ctgaaaaaat catatagcac tgatattcat ctttggcata acgggaaatg ggataaatcg
     1801 ggtgaattat cgcaaggtcg ggcctacgga gtatcattgc cctggaataa tagtttatta
     1861 attattggcg gtgaaactgc aggcggcaaa gcggtgacgg attcagtttt aatttctgtg
     1921 aaggataata aagtcacagt acaaaactaa cgcttcaggg ccccggtaaa ggggccctgc
     1981 tatcaatttg tcatttgtat tacgcgaaaa ttatatgaac gcaataatat cgcccgatta
     2041 ttactatgtt cttaccgttg ctggtcagtc taatgccatg gcgtatggcg aaggactgcc
     2101 attaccggac agggaagatg cgcctcattc cagaattaaa caattagcga gatttgcgca
     2161 tacgcatccc ggaggcccgc catgtcactt taacgacatt attccactga ctcactgccc
     2221 acacgatgtt caggatatgc agggttatca ccatcctctg gcaacgaatc atcaaacaca
     2281 gtacggcacc gttggccagg cactgcatat tgcacggaaa ttactgccct tcattcctaa
     2341 taatgcaggg attcttatcg ttccgtgttg ccgtggcgga tcggctttta ccgcgggcag
     2401 cgaagggaca tattcagaac ggcacggagc cagccatgat gcttgtcgtt ggggaacgga
     2461 tactccgctg taccaggatt tagtcagcag aacgcgagcc gcactggcaa aaaatccgca
     2521 gaacaaattc ctcggcgtat gctggatgca aggcgaattt gacttaatga cctgtgacta
     2581 cgcgtcacac cctcaacact ttaatcatat ggttgaagcc tttcgtaggg atctaaaaca
     2641 ataccattct cagcttaata atattactga cgcgccatgg ttttgcggcg ataccacctg
     2701 gtactggaaa gaaaatttcc cgcattcgta tgaagctatt tatggcaatt atcaaaataa
     2761 tgttttagcc aatattattt tcgtcgactt ccagcaacaa ggtgaaagag gactgacgaa
     2821 cgcgcctgat gaagacccgg acgatttaag cacgggatat tacggttcag cgtaccggtc
     2881 accggagaac tggacgacgg cactgcgaag cagtcatttc agcacggcag cccgtcgggg
     2941 gattatttct gacagatttg tagaagcaat tttgcagttt tggcgcgaaa ggtgagcggg
     3001 gagtttattc atgggggatg tccttgttcg ttctcagagt agacaacaaa aaacaaataa
     3061 gatcaaggta atatctcttc acttgcgggc tcgaacagca ggcaattgcc aatataataa
     3121 tctggcacaa gggtgaaaaa cgcgcaccgc ataaaccgtt gttattgctt acagattagc
     3181 cggatgcctt aatagacatc cgcgcctttt cgattatggc tc
//
LOCUS       DAEEKQ010000292         3206 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.298, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000292 DAEEKQ010000000
VERSION     DAEEKQ010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3206)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3206)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3206
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.298"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(60..587)
                     /gene="mobB"
                     /locus_tag="KLK57_004209"
     CDS             complement(60..587)
                     /gene="mobB"
                     /locus_tag="KLK57_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HBE0894413.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTEKPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(569..1153)
                     /gene="mobA"
                     /locus_tag="KLK57_004210"
     CDS             complement(569..1153)
                     /gene="mobA"
                     /locus_tag="KLK57_004210"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HBE0894414.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHVLASRLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            1223..1492
                     /locus_tag="KLK57_004211"
     CDS             1223..1492
                     /locus_tag="KLK57_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HBE0894415.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            1569..2555
                     /gene="srkA"
                     /locus_tag="KLK57_004212"
     CDS             1569..2555
                     /gene="srkA"
                     /locus_tag="KLK57_004212"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HBE0894416.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQTEAVGRYLGRMHQTGHKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFGEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            2572..3198
                     /gene="dsbA"
                     /locus_tag="KLK57_004213"
     CDS             2572..3198
                     /gene="dsbA"
                     /locus_tag="KLK57_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HBE0894417.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
BASE COUNT          820 a          773 c          778 g          835 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc ggatgcgaca ctggcgcgtc ttatccagcc tactcttgct
       61 tatccgttct gcttttgcat ccactccacc acaaaatcag ccagcccctc aacatcatta
      121 atatccagta atgcgacatc aagattaagc ggcacatcac tggctacagc aataacatgc
      181 ctgtctatca ctaattcttc aggtcgatgt ccggctccat cgcgaaacag cacaatcttt
      241 gcgatctctt catgcttaaa cccttcgacc agaatcaaat ccagctttga ggtatccatt
      301 cgacttgcga gaaactgtag atccagctct tcttcgtctg gtttttccgt catcaaggcc
      361 catcgttgct ggctggcaac gatggtttgt gccgcgccag ccttgcgcag ctcatagcta
      421 tctttgcctg gcttatcaac atccatatca tggtgcgtat gcttaatcag ccctggacgg
      481 atccctctgg cgcataatgc cgggatcagt tttttcaaaa gcgtagtttt tccggtacca
      541 ctccacgcgg caaaggcgag taacggtatc atcgtttttc ctgccatcgg gcaagctcct
      601 ctggcgtatt cacgttaaca aatgcatctt tatgatcgct gaaatcaacc gcatggccgc
      661 cagccagacg cataaatgcc attacccggc gttctcctgc ttgcagatat tctagtaata
      721 aaggctcaat agcacggttt accagagcaa tagtcgggtg atcgcgttca ccgtcatgga
      781 cccacacgac aggcgcatct ttgcgctgat gattaagccg ggaagctaaa acatggggaa
      841 tgtaaggcgt atcgcacgga caaaacaaaa accactcacc agcttcctgc tgcattactg
      901 aaagcattcc tgccagaggg cctgggtaat ccgccagtga atcttcaatc actttcagac
      961 cgcttgcctg atagatttcc tgatgacgat tggcattaac cacgacgtga gagagctgcg
     1021 tcataagcgc gtcagcgaca tgttgccata atggtttgcc gtttaattca agcaatcctt
     1081 tatctacgcc gcccattcgt ctggctttac cgcctgccag cacaacgcct gttatcgtcg
     1141 tcatcagatt caccgatatc gcctctttta ttgtgggatt gaccctgcta acgtgtctgt
     1201 ctcaagagta aggagcatca ctatgaaatg taaacgtctg aatgaagtta ttgaactcct
     1261 ccagccagcc tggcaaaaag agccagacct taacctgttg caatttttgc agaaactggc
     1321 gaaagagtca ggttttgacg gcgaactggc ggatttgacg gatgacattc tgatctatca
     1381 cctgaaaatg cgtgattccg cgaaagatgc ggtgatcccg ggtttgcaga aagattatga
     1441 agaagacttc aaaacggcgc tgttacgcgc tcgcggcgta attaaagagt aaaagcttgt
     1501 aagcggcgcc accaaaatca tcgtgaaatg atatccttca tcattcgtaa tgttttccgg
     1561 atgatgggat gaacaacagc gcttttactt tccagacact acacccggat accatcatgg
     1621 acgctctgtt tgagcaaggg atccgggtgg attccggtct taccccgctt aacagctatg
     1681 aaaaccgtgt ctatcaattt caggacgaag agcgtcgacg ttttgtcgtc aaattttatc
     1741 gccctgaacg ttggacagcc gatcaaatcc tcgaagaaca tcaatttgcg ttgcagctgg
     1801 taaatgatga agttccggtc gcagcacctg tggcctttaa cggtcagact ttattgaatc
     1861 atcagggatt ttatttcgct gtttttccaa gcgtcggtgg tcgccagttc gaagctgata
     1921 atatcgatca gacggaagcg gttgggcgtt atttagggcg tatgcaccag acggggcaca
     1981 aacagctttt tatccatcgc ccgaccatcg gtctgaatga atatctcatt gagccacgca
     2041 agctgtttga ggacgctaca ctgatacctt ccgggttgaa agcggcattc ctgaaagcga
     2101 cagatgagct gatcgccgcc gttacagcac actggcggga agatttcacc gttctgcggc
     2161 tacatggaga ctgccacgcc gggaatattc tctggcgcga tggtccaatg tttgttgatc
     2221 tggatgatgc acgtaatggt ccagctattc aggatttgtg gatgttgctc aatggcgata
     2281 aagccgagca gcggatgcaa ctggaaacta ttattgaagc ttatgaagaa tttggcgagt
     2341 tcgacaccgc tgaaatcgga ctgattgaac ctttacgcgc catgcgtttg gtttattatc
     2401 ttgcctggct aatgcggcgt tgggctgatc ccgcgttccc gaaaaatttc ccgtggttaa
     2461 ccggggaaga ttactggctg cgacaaacgg cgacttttat agaacaggca aaagttctac
     2521 aagaaccccc tttacaatta acacctatgt attaatcgga gagagtagat catgaaaaag
     2581 atttggctgg cgctggctgg tttagtttta gcgtttagcg catcggcggc gcagtatgaa
     2641 gatggtaaac agtacactac cctggaaaaa ccggtagctg gcgcgccgca agtgctggag
     2701 tttttctctt tcttctgtcc gcactgctat cagtttgaag aagttctgca tatttctgat
     2761 aatgtgaaga aaaaactgcc ggaaggcgtg aagatgacta aataccacgt caacttcatg
     2821 ggtggtgacc tgggcaaaga tctgactcag gcatgggctg tggcgatggc gctgggcgtg
     2881 gaagacaaag tcactgttcc gctgtttgaa ggcgtacaga aaacccagac cattcgttca
     2941 gcatctgata tccgcgatgt atttatcaac gcaggtatta aaggtgaaga gtacgacgcg
     3001 gcgtggaaca gcttcgtggt caaatctctg gtcgctcagc aggaaaaagc tgcagctgac
     3061 gtgcaattgc gtggcgttcc ggcgatgttt gttaacggta aatatcagct gaatccgcag
     3121 ggtatggata ccagcaatat ggatgttttt gttcagcagt atgctgatac agtgaaatat
     3181 ctgtccgaga aaaaataatt caatgt
//
LOCUS       DAEEKQ010000293         3174 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.299, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000293 DAEEKQ010000000
VERSION     DAEEKQ010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3174
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.299"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..339
                     /locus_tag="KLK57_004214"
     CDS             <1..339
                     /locus_tag="KLK57_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011310189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894418.1"
                     /translation="SDLEVSSKKTSKQDREITDVSVDSDHKNTQCPATDDELDPFDDE
                     EDEREKAIKRAEKKKRFELARQKNKVSGLDPFDDSEDDYYLSPKKKQTQDDKKQKIKP
                     KMYFAGGGIK"
     gene            339..629
                     /locus_tag="KLK57_004215"
     CDS             339..629
                     /locus_tag="KLK57_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000666098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894419.1"
                     /translation="MKEYHGEKRYKDYLLKRYSISREGHLMKDTHGEVYRIRPKKEGR
                     GYFFYDGVTDLKIDALRFAVLYHFDIWNSIHQLRLKDGDPSNLKATNIITKR"
     gene            950..1240
                     /locus_tag="KLK57_004216"
     CDS             950..1240
                     /locus_tag="KLK57_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000149937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894420.1"
                     /translation="MRHTVTRLLPEAYSRLAAIKDKYPAMSHTALVGEILKEHIALTP
                     FDAIKGVAEGSKAIRITTGQLELLKAQSAVAGCSMIQVLSAKVHQLADKLGV"
     gene            1240..1659
                     /locus_tag="KLK57_004217"
     CDS             1240..1659
                     /locus_tag="KLK57_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894421.1"
                     /translation="MLCTFDYHAFYRHRGHLVRENNGTWKDLTRNAEPFTHQQLKSRF
                     SRWKNAPADTGDFEIIHTFDYASEDDLHDAIKLIEDFNTSEDVDGDKYKSRGNRYNRD
                     DITQAINAGQSQRYITEKYGISRRQYFYIKPLINKDN"
     gene            1827..2150
                     /locus_tag="KLK57_004218"
     CDS             1827..2150
                     /locus_tag="KLK57_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894422.1"
                     /translation="MNNYPMQIMVDNDTAMMVQSFIDAGIAINFDKLLKLMADNVENI
                     SDFIQSVEFNEPRMMLPITDSNMKRLVIEQTNIYSISPEQFLKGAVTILYSDNILVTD
                     SVRVH"
     gene            2580..>3174
                     /locus_tag="KLK57_004219"
     CDS             2580..>3174
                     /locus_tag="KLK57_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001461099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894423.1"
                     /translation="MIRNEHEMLIEHRPSALDIHELTLRESFDRAWRESHEVNKGPYI
                     PPEPLEIPRVEIDFSMNERCELDLKLKLQRRYFQAIKDSQEAMRSVYEKIQSKLEDEI
                     DEMVISMELQTNSYAYFSRLDRDGWGYDPVEIGKVIADLPEGQRVVRTERAVRAPHRL
                     SLIVADKSQAELEEIAKEKLMKARENEINLLKSRLAKL"
BASE COUNT         1055 a          570 c          665 g          884 t
ORIGIN      
        1 tctgaccttg aagtaagctc taaaaagact tctaaacaag acagagaaat cactgatgtg
       61 agtgttgata gtgaccataa aaacacacag tgccccgcca ctgatgatga gttggacccc
      121 ttcgatgatg aagaggatga acgtgaaaaa gccattaaga gggcagagaa gaaaaagaga
      181 ttcgagcttg caagacaaaa gaacaaagta tcgggtttag acccattcga tgacagtgaa
      241 gatgattatt atctttcacc gaaaaagaaa caaactcagg atgacaagaa gcaaaaaatt
      301 aaaccgaaga tgtattttgc cggtggagga attaagtaat gaaagaatat cacggtgaaa
      361 agagatacaa agattacctg ttaaagcgtt acagcatttc tcgtgaaggt catcttatga
      421 aggatactca cggggaagtt taccggatta gaccaaagaa agaagggagg ggttatttct
      481 tttacgatgg tgtgacagat ttaaagattg acgctttgag gtttgcagtg ctgtatcatt
      541 ttgatatctg gaattcaatc caccagctac gattaaaaga cggtgacccc agcaacttaa
      601 aggccaccaa tattatcaca aaacgttagt ttgttttacc atcatcaatc gtaattgtga
      661 tttcatattt gaaaagctta atgctgaaat tgaatcgtgt acttctggcg ataatcattt
      721 ttaaaagctg atacacaaga agcaccagaa acacaaagca acctaagctt gcgaatctgt
      781 gagattcagc aattgtttca agaaagccca ttaattcccc ttatttatca aagggaatga
      841 cctgtatcag ccccctacaa ccatattaac atgtaaatga gttgttgaca atatgttata
      901 aatatgttaa agttagttat aagtcaaaga ttttaagaag gaaaatttaa tgagacacac
      961 agttacacgc cttttgccag aggcatattc cagactggca gcaatcaaag acaaataccc
     1021 agccatgagc cacacagcac ttgttggtga gattttgaag gaacacatag cccttacacc
     1081 gtttgacgct atcaagggcg ttgcagaggg ttctaaggcc atcagaatca caacgggcca
     1141 actggaacta ctcaaagcac agtccgcagt ggcaggttgc tcaatgattc aggtgttgag
     1201 tgccaaggtt catcaactgg cggataagct gggggtttaa tgctatgcac atttgattat
     1261 catgcgtttt atcgccatag aggtcacctt gtccgtgaga ataacggaac atggaaagat
     1321 ttaactcgta atgctgaacc cttcacacac cagcaattaa aatcccgctt ctccagatgg
     1381 aagaatgcac cagcagatac tggtgatttt gagattatcc ataccttcga ttatgcctca
     1441 gaagatgacc ttcacgatgc aattaagctg attgaagact tcaatacctc agaagatgtg
     1501 gatggtgaca aatataaatc ccgtggtaac cgctataacc gtgatgatat cactcaggcc
     1561 ataaatgctg gtcaatctca gcgatacatt acagaaaaat atggtatttc acggagacag
     1621 tatttctaca ttaagccatt gattaataaa gacaattaaa aataaccatc taagaaacgg
     1681 tagaatgcac tttacaggca gagttgcacc ttaatttgat aaattacctt accagccagt
     1741 gcaactccct ctgtaacccg catggttact ggcctcagaa tggctatttt aagaggtctg
     1801 aggtgtgttt cataggagat ttaaaaatga ataattaccc aatgcaaatt atggttgata
     1861 acgacactgc tatgatggtg caaagcttca ttgatgcagg tatcgcaatt aattttgata
     1921 aattactaaa gctgatggct gataatgtag aaaacatctc tgactttatt cagtcggtgg
     1981 agtttaacga gcctcgaatg atgctaccaa ttacagacag caacatgaaa agattggtta
     2041 tcgagcaaac gaacatatac agtatttccc cagagcaatt tttgaaaggt gctgtgacaa
     2101 tcctgtacag tgataatatt ctggtgacgg attctgtgag ggttcactaa tgggtttaat
     2161 aattgcttgg acgttgcttg gcttatttgt tggttgtatt attaacaatg cggtaaataa
     2221 gaattgatgt tatcaacctg ttaaaaagct gttgacaaat aaccttaagc atggcataat
     2281 tttaattatg ggagagcaat tctctcttat ttttctttca ataatccgat ggatggatat
     2341 tctgagttgg aaaggtgcaa tgcttttcgt catggccttt caggttagtc tgagaggcta
     2401 tctcaaaaag tactcttgtt cattcattat tgaatccttt gcccttcaca gaaatgttta
     2461 gggctttttc agtttttaaa agcacacttg gcaggttctt gttcagtcag actcttcacc
     2521 tctgattgct gagtgtgctt ctataaattg aaataacaaa taaactaagg ggtgaacgaa
     2581 tgattagaaa tgaacatgaa atgcttatcg aacacagacc aagtgcactt gatattcacg
     2641 aattaacttt gcgtgaatct tttgatagag cttggcggga atcacacgaa gtaaataaag
     2701 gtccatatat tccaccagaa ccactcgaga ttccaagagt tgaaattgat ttttctatga
     2761 acgaacggtg tgaacttgat ttgaagttga agctacaacg ccgatatttc caagctatta
     2821 aagactctca agaagcaatg agaagtgttt acgagaaaat tcagtccaag cttgaagacg
     2881 aaattgacga aatggttatt tcgatggagc ttcaaaccaa ttcttatgct tatttctctc
     2941 gactagaccg tgacggttgg gggtatgacc ctgtagagat tggtaaagtg attgcagatt
     3001 taccagaagg tcaaagagtt gttcgtacag aaagagctgt cagagcacca catcgactgt
     3061 ctctaattgt tgctgataaa tcacaagctg agcttgaaga aattgccaaa gagaaactga
     3121 tgaaagcaag agaaaatgag attaatttgc tgaaatcaag actggctaag ctga
//
LOCUS       DAEEKQ010000294         3172 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.300, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000294 DAEEKQ010000000
VERSION     DAEEKQ010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.300"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            530..2383
                     /locus_tag="KLK57_004220"
     CDS             530..2383
                     /locus_tag="KLK57_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HBE0894424.1"
                     /translation="MAFKHYDVVRAASPSDLAKRLTQKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPEWYYVIVLAGQSNAMAYGEGLPLPDSYDAPHPRIKQLARRN
                     TVTPGGEVCVFNDIIPADHCLHDVQDMSTINHPRADLSKGQYGCVGQGLHIAKKLLPY
                     IPNNAGILLVPCCRGGSAFTQGTEGTFSESTGASQDSARWGVGKPLYQDLLFRTKAAL
                     QKNPKNVLLAICWMQGEFDMTNASYAQQPAAFLAMVQQFRADLAGLAAQCHGGSPASV
                     PWICGDTTYAWKQEHGTQYEVVYGAYKGKESQQIYFVPFMTDGSGVNTPTNNPSEDPD
                     IAGSGYYGSASRTNKNWVSSNRPTHFSSWARRGIIPDRMATAILNVAGRTLAFISGKA
                     PEIKPSPGGDTPSGPSEDASIRTISLLPTAGDAAAQGWSIKNGGVQLSDGVFKITKQS
                     NKAWSLTRPVDDAVSLLTRGGRLSCKFRLSGALTNNQFGLGIYLYTDVALPDVVAMTG
                     TGNPFLMSFFTQTTDGKLNLMHHKKAGNTKLGEFGNYSNDWLTLELVFTAGSATVTPK
                     LNGVAGPAFQVIKDSLTLGLNALTLTDITKNAAYGVEIESLVLEINAPASS"
     gene            2534..2749
                     /locus_tag="KLK57_004221"
     CDS             2534..2749
                     /locus_tag="KLK57_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HBE0894425.1"
                     /translation="MYQMEKITTGVSYTTSAVGTGYWFLQLLDRVSPSQWAAIGVLGS
                     LLFGLLTYLTNLYFKIREDRRKAARGE"
     gene            2754..3068
                     /locus_tag="KLK57_004222"
     CDS             2754..3068
                     /locus_tag="KLK57_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HBE0894426.1"
                     /translation="MTQNYELIVKGIRNFENKVTVTLALRDKKRFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELLPETRPCSEARYTIKINSSDNSIT
                     GC"
BASE COUNT          778 a          761 c          885 g          748 t
ORIGIN      
        1 ccaggcccat ccgctccggt gggccttttt actgcagaaa acaggttccc cgttaaatgc
       61 tatgttgctc acaattcagt aagttgacag ttgcctgtca gactgggcat ttgttaaaaa
      121 aatttcgcat ggtgaatccc cctgagcgga ggggcgactg gtgacggtat aatctctgat
      181 tatcaaaacg agaatgacgc gggtttagtg gcaccgggct gaactcaccg ggaggcaccc
      241 ggcaccatgt gcatgatgat acagatacgc ggctttagcc cctctccgga ggggttttct
      301 tgtgggcaaa aaaagcccgc gctgggagac gcgggcggca aggaataaac aataaaacgt
      361 gaagtaatat ttcagctggc gaataatacc ccatagtaat cactctgcgc aactgcgcgg
      421 tctttttcga attgcgggct gtcgtctctc ttctgccatt gtcctgtaac ttccggactt
      481 cagcccgctc cttattttac tcacaatatt atcccggccg ggaggattca tggcatttaa
      541 acactatgac gtggtcaggg cggcatcgcc gtcagacctt gcgaaacgac tgacacaaaa
      601 actgaaggag ggctggcagc cgtttggtag tccggtggcc ataacccctt ataccctgat
      661 gcaggcgatt gcagcagaag gtgatgtggt cgtcagtggt gcaactgagc cggagtggta
      721 ctacgtcatc gtactggccg ggcaatccaa tgccatggct tacggtgaag gtcttccgct
      781 tccggattca tacgatgcgc cccatccgcg cattaagcaa ctggcccgtc gtaacacagt
      841 gactcccggt ggtgaagtat gcgtatttaa cgacatcatt cctgctgacc attgtctgca
      901 tgatgttcag gatatgagta cgattaacca tccccgggct gacctgagca aagggcagta
      961 cggctgtgtc ggacagggct tacatattgc caaaaaactg cttccgtata tccctaataa
     1021 tgcggggatc ctgctggtac catgctgtcg tggtggttcg gcattcaccc agggcacgga
     1081 ggggacattc agcgagtcca cgggggccag tcaggattcg gctcgctggg gagtgggtaa
     1141 gccgttatat caggatctgc ttttccgcac gaaggcagca ttgcagaaaa acccgaaaaa
     1201 cgttttgctg gcgatatgct ggatgcaggg ggaattcgat atgacgaatg ccagttacgc
     1261 ccagcagcca gcagcatttc ttgcaatggt acagcagttc cgtgctgacc ttgccgggct
     1321 ggcggcgcag tgtcacggtg gaagtccggc atcagtcccc tggatttgtg gcgacacgac
     1381 atacgcgtgg aaacaagaac acggtacgca atatgaagtg gtatatggtg catataaagg
     1441 taaagaatcc cagcagattt attttgttcc ctttatgacc gatggtagcg gagttaatac
     1501 accgacaaac aacccgtcag aagatcctga tattgccggg tctggttatt acggttcggc
     1561 atcccgaacg aacaaaaact gggtatcatc aaatcgcccg acgcatttca gctcatgggc
     1621 gcgtcgtggc attattcccg atcgtatggc aactgctatt ctgaacgtag ccggtcgcac
     1681 cttagccttc attagtggta aggcaccgga aatcaaaccc tcgcccggcg gcgacacgcc
     1741 atcggggccg tctgaagatg catccatacg cacaatctcc ctgttgccga cagccggaga
     1801 cgctgctgcg cagggctgga gcattaagaa tggcggagtt cagttgtcag atggtgtatt
     1861 taagatcacc aagcagagca ataaagcctg gtccctgacg cgcccggtgg atgacgcagt
     1921 ctccctgctg acacggggtg gcagactgag ctgtaagttt cgactgtcag gcgcactgac
     1981 caacaaccag ttcggtctgg gaatttatct gtataccgat gtagcgttac ctgacgtcgt
     2041 ggcgatgacc gggactggta acccgttcct gatgtcgttc ttcacccaga ccacagacgg
     2101 caaactgaat ctgatgcatc acaagaaagc aggaaacaca aagttgggcg agttcgggaa
     2161 ttacagtaac gactggctga cgctggagct ggtgttcacc gccggcagtg ccacggttac
     2221 tccgaaactg aatggagtgg ctggcccggc attccaggtc ataaaagaca gtctgacact
     2281 ggggctgaat gcgctgacgc tgacggatat taccaaaaat gcagcgtatg gcgttgagat
     2341 agaaagtctg gtgctggaga taaatgcacc ggcatcatca taaaaagtga gccagccaaa
     2401 tggaaggtat cgttaaactc accggtagtg tcagtgggtc gtctgagatg cctgcatgag
     2461 ttatcagagc catcagtact taactggtgg ctttttttat tgttgtcagc ttccggataa
     2521 cgggagacgg ggtatgtacc agatggaaaa aatcacaaca ggtgtgtcat acaccacgtc
     2581 agcggtggga acgggctact ggttcctgca gttgctggac agggtttccc cgtctcagtg
     2641 ggcggcaata ggcgtgctgg ggagtctgct gtttgggctg ctgacatatc tgactaacct
     2701 gtatttcaaa atcagagagg accgtcgtaa ggctgcacgg ggagagtaat tcaatgactc
     2761 aaaactatga actgattgtg aaagggatcc gcaattttga gaataaagtt acggtaactt
     2821 tagcgttacg ggacaaaaaa cgctttgacg gtgaaatttt tgacctggac atctcgctgg
     2881 accgtgttga aggtgccgcg ctggagtttt atgaggcagc agccagaagg agcatcagac
     2941 aggtcttcct ggatgttgct gccgggttat gtgaagggga cgagctgttg ccagaaacgc
     3001 gcccctgttc agaggcgcgg tataccataa aaattaacag ttctgataac tcgattacgg
     3061 gttgttagct ttttgcagtt ggctttccag tatctttcat tggtagcatc ctgataaata
     3121 tccatgagcg caaaaatcaa atacggcctg tcagctgctg ttctggcgct ga
//
LOCUS       DAEEKQ010000295         3163 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.301, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000295 DAEEKQ010000000
VERSION     DAEEKQ010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3163
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.301"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(162..237)
                     /locus_tag="KLK57_004223"
     tRNA            complement(162..237)
                     /locus_tag="KLK57_004223"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(202..204),aa:Asn,seq:gtt)
     gene            complement(338..1981)
                     /gene="yeeO"
                     /locus_tag="KLK57_004224"
     CDS             complement(338..1981)
                     /gene="yeeO"
                     /locus_tag="KLK57_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HBE0894427.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRSEPQKCEKKPVVSE"
     gene            1986..2061
                     /locus_tag="KLK57_004225"
     tRNA            1986..2061
                     /locus_tag="KLK57_004225"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2019..2021,aa:Asn,seq:gtt)
     gene            complement(2099..3049)
                     /gene="cbl"
                     /locus_tag="KLK57_004226"
     CDS             complement(2099..3049)
                     /gene="cbl"
                     /locus_tag="KLK57_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HBE0894428.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
BASE COUNT          933 a          831 c          667 g          732 t
ORIGIN      
        1 ttggggaggg gcaatttgct cactgaagcg tgagactcga ttaagcgcac gaaacacaga
       61 aatcaaaaaa cccggtcact tttttacaag gtaaccgggt aaaaataatt tttatttttt
      121 aactgttttg agactcatag agatgtctca aaactaaaat ttggctcctc tgactggact
      181 cgaaccagtg acatacggat taacagtccg ccgttctacc gactgaacta cagaggaatc
      241 gtgagaacga ggcgaatatt agcgatgccc acccacaatg tcaaagcctg ttttttaaat
      301 ttgaaatcgt ttgctgaaat aatctgcatt ttgtcgttta ttccgacaca actggctttt
      361 tttcacactt ttgcggttcg gatcgagggt atttccacag ccaacgtcca gtaaccattc
      421 gccagtaaaa cagcacggcc cgcacagccc agtcggcaaa cattcccatc cagacaccaa
      481 ccacacccca gccaagcatg atgcccagca cataaccgac gacaacccgg caaccccaca
      541 tgctcaacat agaaacccac atggcgtaac gggcatcgcg agcaccttta aatccagcgg
      601 gtagcaccca tgaggcggac caaataggca taaataaagc atttagccaa atcagaatca
      661 caacgacatg tttaacctgt ggatcctggg tgtaaaacga tgccataacc ccggcaaagg
      721 gagccgttag ccaggcgatg gccgttaatc caagagtgga aagccagaac acatgccgca
      781 actgaatctc tgcttgcgct atctgcccta cccccaacct tcggcctgta atgatcgtag
      841 aagcagagcc gagcgcactt ccgggtaagt tgataagagc cgcaattgaa aacgcgataa
      901 aatttccggc aataacactg gtccccatcc cggcaacgaa catttgggtt aataaccgac
      961 cactggtaaa taacactgat tcgacactcg cgggaatacc aatccccatg acttcccaga
     1021 taatgctaaa attcagcggt ttaaaatagc tctttaacga aatccttagc gcaggattaa
     1081 aaccaatcgc cagcacccac aaaattgcaa ctgcgccaat ataacgagaa atggttaaac
     1141 ccagccctgc cccgacaaat cccagtcccg gccaggagaa aaggccgtaa atcaatatgc
     1201 cgctaataat aatattaaga atattcaggc taccgttaat caatagcggt attttcgtat
     1261 tccctgcacc acgaagtgcc ccgctaccaa taagagtgat ggcagctgct ggataactga
     1321 gtaccgtcag ctccagataa gtcaacgcca gtgctttaac ttctgtcgtg gcatcacccg
     1381 cgacgaaatc aataatttgt tcgccaaaat gatgaataag cgttgccaac agtacggcaa
     1441 acaacgtcat gatcaccaat gactgccgcg tcgccaccct cgctcgtcgt cgatcccgct
     1501 taccgagact aaatgccaca acgacagtag taccaagatc gatagcagca aaaaaagcca
     1561 taatgaccat attgaagctg tccgccaatc ccacgccggc catcgcatct tttcccagcc
     1621 agctgaccag aaaagtgctc agaaccccca tcaacaggac acaggcattc tccatgaaga
     1681 taggaacagc aagcggggtt atctcgcgcc agaacaacac tttgtagctc ttgcgtttag
     1741 cgtgccagcg agtgccgtga acaacctggc gtaaagcaga ggagatattc aaagccgacc
     1801 ttaattgcag aaagtgaaac cacatttcaa ataatgaggg agaatcagca aagctgcaaa
     1861 gattttcgcc aacaaattgt ctgcaaatgc aacaaactgt tgatagaaac ggcaaacagt
     1921 tggggaattt aaaaatcggg tttgacaaaa gatttttcgc cgttaagatg tgcctcaaca
     1981 gcgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat gtcactggtt
     2041 cgagtccagt cagaggagcc aaatttctga aaagcccgct tttatagcgg gctttttgct
     2101 atatctgata atcaatttcc tcttcactgt tttccatcac ctgccgcttg atatcctcaa
     2161 ctgacagtcc tgcattacaa agttccagaa agcgccagac atagttacgc tgaagttgtc
     2221 ctcgcttcag tcccaaccag acagtattag catcgaaaag atgccgcgta tccagacgga
     2281 ttaaattctc ttcctcttgt tcgccgctgg attgttcggc aactaatccg atcccaagcc
     2341 caagagcaac ataggtttta atgacatcag aatcctgcgc acttaataca atatctgcca
     2401 gcaaaccttt gcgggcaaat gcgtcatcaa tacgtgagcg ccccgtaatc ccctgtcggt
     2461 aagtgattaa cggccacttc gctattgatt ccagcgtcaa tggtgtaatt tgcgttaagg
     2521 gatgatcgag tggaacaagc aaactatggt gccaacgaaa ccacgggaag gcgacgagct
     2581 gcgggtcatt actcaaacgc tcgctggcga taccaatatc agcttcgcca ttttgcaaca
     2641 acgtcgcaat ttcctgtggc gtcccctgga ttagctcgag ccgaacctcc gggaaaagtt
     2701 cgcgaaaagc tttaatgacc tctggcaagc tataacgtgc ctgagtatgc gtcgttgcaa
     2761 tagtgagaac gccagacgta tcgttggtaa acaggtctgc aagccgacga acattactgg
     2821 cttcattcag aatacgttct gcaatgacca gtaatgcttt gcccggttca gtcatgccca
     2881 gcagtcgctt acctcgtcga acaaatattt cgatgccaag ttcatcctcc agttcccgaa
     2941 tatgacggct gacgccggac tgtgaggtaa aaagcatatt cgcaacctct gtcaggttgt
     3001 aatcctgacg tgcagcctcg cggattatct ttagttgttg gaaattcacg gtaaactccg
     3061 ggcagttcag atttcccgtt attgttaaag tctaatgccc ggcataacaa ataataaaaa
     3121 cccgcatctt attccatccc gatataacgc ttagctcacc aat
//
LOCUS       DAEEKQ010000296         3109 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.302, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000296 DAEEKQ010000000
VERSION     DAEEKQ010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3109)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3109)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3109
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.302"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1255)
                     /locus_tag="KLK57_004227"
     CDS             complement(<1..1255)
                     /locus_tag="KLK57_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HBE0894429.1"
                     /translation="MAQFKVDDFLIELSFNSQKVLKGLEKAEKQTMQVASRIEKRLNK
                     SFRVNPTPLNDSLKLMERNVDKTVSKIEQRLKNTRAFKIKTEIEDTLKPLRQPRQPRI
                     SGSRAITAAYSANMSKLRDFNPMLEKYFKSQYYSLSSKSKTLGNETFNRELAKLNQSL
                     RETLSKFNKTTSKNNHSENASNSLDVLATSAIKAGTAIYSFQTALEAYRRIMEVGLKK
                     EASQRAAQFVLGDEGAKRATEFVKNLADSSGVDQIETLSSFAKFSAGAGDMNADQKES
                     LFSNVIGTSRLMGLSTDEINGILKAFEQMASKGKIQAEELRGQLGDRMAGAFQLFARS
                     LGMTTEELDKAMKDGKVLSKDVFPKVSAEMGRMIDKAGGWEKIINSTQTQLGRLSNSW
                     NNNLALMFDGSQEGLTDFTRSLTNLL"
     gene            complement(1412..1621)
                     /locus_tag="KLK57_004228"
     CDS             complement(1412..1621)
                     /locus_tag="KLK57_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894430.1"
                     /translation="MFEERLETIRSVCENLKLQTKPALRIKNKRQVITSHKPKTRKIP
                     KWCIDRIPSDAQIIGETELHYLVRH"
     gene            complement(1644..2354)
                     /locus_tag="KLK57_004229"
     CDS             complement(1644..2354)
                     /locus_tag="KLK57_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0894431.1"
                     /translation="MTNDLYINGKKVDSFGDWSPQGEHPLHTIQRQEHDKRIELEQEL
                     KMSPKVISFVSPEPQEMPDICKTEALFELEREYYPALKSQRLRLDVAYDAVKNFEETS
                     KPSEYDIAYEIKSNPFIYYEGNFNDGFGTAIEDVPKVLQSIPEGFRLIDVRQALRGSG
                     QFILMTDKTDEEILQLAEANLTKHYSGKLDKLKQKLKKELDTMKELVSEYESQKKIAL
                     MADIEQLTKISQKYSKAV"
     gene            complement(2870..>3109)
                     /locus_tag="KLK57_004230"
     CDS             complement(2870..>3109)
                     /locus_tag="KLK57_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894432.1"
                     /translation="DLDKLLKLMAENSEEIEDFIQEIETGEPRFMFPVTDSNMKRLII
                     EETNKYSVSPEKYLKAAIAILYSDNILVTDSKVVH"
BASE COUNT          911 a          654 c          507 g         1037 t
ORIGIN      
        1 taagtaaatt agtcagtgac cttgtaaagt ctgttaaacc ttcctgtgaa ccatcgaaca
       61 ttaaggccag attattattc catgaattac ttaagcggcc taactgcgtc tgagtagagt
      121 taataatctt ttcccatcca ccagctttat caatcatcct tcccatttca gcggatactt
      181 taggaaaaac atctttagaa agaaccttac cgtctttcat ggccttgtct aattcttccg
      241 tagtcatccc aagacttctg gcaaataact gaaaagcacc tgccattctg tcacctaatt
      301 gaccgcgtag ctcttcagcc tgaatctttc ctttagatgc catctgctcg aaagctttta
      361 aaatcccgtt aatttcatcg gtgcttagcc ccattaatct ggatgtacca atcacattag
      421 aaaataagga ttctttctgg tctgcattca tatcaccagc accagcggaa aacttagcaa
      481 aactgcttaa tgtttcaatc tggtctacac cactactatc agcaagattc ttaacaaatt
      541 ctgtagctcg tttagcacct tcatcaccta aaacgaattg tgccgctcgt tgtgatgctt
      601 cttttttcag accaacctcc ataatccgcc tgtaagcttc taaggctgtc tgaaaactat
      661 aaatggctgt acctgctttg attgcactcg tagctaatac atccagacta tttgaagcat
      721 tttcactgtg attatttttg ctggtggtct tgttaaactt agaaagtgtt tcacgaagag
      781 actgatttaa ctttgctaac tcacgattaa aagtttcgtt tccaagtgtc ttggatttac
      841 tgcttaagct gtaatactgg cttttaaaat acttctcaag cataggatta aagtcacgaa
      901 gcttgctcat attcgctgag taagctgctg tgattgctct gcttccgcta attcgtggct
      961 gtcttggttg tctaagtggc tttaaagtat cctcaatttc agttttaatc ttaaacgctc
     1021 tagtgttttt aagacgctgc tcaatcttgg aaacagtttt atcgacattt ctttccatta
     1081 gtttgagaga atcatttaaa ggcgtaggat taactctaaa cgacttattc aatcgcttct
     1141 caattcgtga tgctacctgc atcgtttgtt tctcggcttt ttccaagcct tttaaaactt
     1201 tctgagagtt aaaacttaac tcaatcagaa aatcatcaac tttaaattgt gccattcata
     1261 tttttcacct ctttaagcgt gttgtacgta aattctattt ttcagtgtgg gtattagctt
     1321 tacagatttc acgtagcaca catacaaaaa aagggagagc taccgatgtc actcgataat
     1381 ctctccccgt atttacccaa ccaattcttt tttaatgtcg gacaagataa tgtaattccg
     1441 tttcacctat gatttgagca tcagaaggaa tacggtcaat acaccacttt ggaatcttgc
     1501 gtgtttttgg tttgtgtgag gtgataactt gacgtttgtt tttaattctt aaggctggct
     1561 tagtttgtaa ctttaagttt tcacaaacag accgaatagt ctctaaacgt tcttcaaaca
     1621 ttaatcacct ctttaattca taatcaaaca gccttgctat acttctgaga aatcttcgtt
     1681 aactgctcaa tatcagccat caaagcaatc ttcttctgcg actcatattc actaacaagt
     1741 tctttcatag tatcaagttc ttttttaagc ttttgcttta acttatccag tttaccggaa
     1801 taatgcttag tcaaattagc ttcagctaac tgaagaatct cttcatctgt tttatcagtc
     1861 atcagaataa attgaccaga accccttaat gcttgtctga catcaattaa ccggaaccct
     1921 tcagggatac tttgaagaac tttaggaacg tcttcaatcg ctgtaccaaa accatcatta
     1981 aagtttcctt cataatagat aaacgggttt gacttaatct cgtaagcaat atcatattct
     2041 gaaggtttcg aggtttcttc gaagttctta acagcatcgt aagcgacatc aagtctaaga
     2101 cgctgtgatt ttaaagctgg gtaatattca cgctcaagct caaatagtgc ttcagttttg
     2161 cagatatcag gcatttcttg aggctcagga gacacaaagg aaattacctt tggtgacatc
     2221 tttagctctt gctcaagctc aatgcgcttg tcatgctctt gacgctgaat agtatgtagc
     2281 ggatgttcac cctgtggtga ccagtctccg aagctatcaa cctttttacc gttaatatat
     2341 aaatcgtttg tcattataat ttcctctaag tgtggttatt tcttaataga gcaccttcaa
     2401 cgccaataca cttagaagat gacttaacga tttattaaag atgctctatt aagaaaagcc
     2461 ctaaacataa tctgtgaagg gctttggagg tttgcttata acgttaacga agtttcagga
     2521 aaactttctt taaaagctct gtttaaaaac tcttaaagaa agccctaact catgattagg
     2581 gcaaagctgt aaaggattca ataatgaatg aacaaagact ttttgagata gcctctcaga
     2641 ccaacctgaa aggctaggac gaaaagcatt gcacctttcc aactcagaat atccattcat
     2701 cggattattg aaagaaaaaa taagagagaa ttgctctccc ccatatatac aattatgtca
     2761 tgctcaaggt tgtttgtcaa cagcctttaa cagattggta acatttatac tttaatagat
     2821 gcacctgcaa tcataccaat caatacaaaa cagataatca ccaattccat taatgcacca
     2881 ccttcgaatc cgtcaccaga atattatcgc tatacaaaat tgctatagcc gccttgagat
     2941 atttttcagg gctaacgctg tatttgttcg tttcttcgat aataagacgc ttcatgtttg
     3001 agtcggtaac aggaaacata aaacgcggtt caccagtttc aatttcctga ataaagtctt
     3061 caatttcttc tgagttttca gccattagct ttagtaattt atcaaggtc
//
LOCUS       DAEEKQ010000297         3094 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.303, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000297 DAEEKQ010000000
VERSION     DAEEKQ010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.303"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1199)
                     /locus_tag="KLK57_004231"
     CDS             complement(<1..1199)
                     /locus_tag="KLK57_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HBE0894433.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSA"
     gene            1492..1586
                     /locus_tag="KLK57_004232"
     tRNA            1492..1586
                     /locus_tag="KLK57_004232"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1526..1528,aa:Sec,seq:tca)
     gene            1886..3070
                     /locus_tag="KLK57_004233"
     CDS             1886..3070
                     /locus_tag="KLK57_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0894434.1"
                     /translation="MALTDIKVKTAKPKDKPYKLADGGGMYLLINTNGSKYWRMKYRF
                     AGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQMSLKNTFEA
                     IAREWHQSKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMELLEALRKMEKRGA
                     LEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTANELPHFLND
                     LAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVMKMKRPHIVP
                     LSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKGRLTGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYADYIDSLSSR
                     ES"
BASE COUNT          899 a          733 c          706 g          756 t
ORIGIN      
        1 gcgctgttct gcgctttttc tgccgcatca tatccgccat aagccagcat ccagccgata
       61 agagcgccgc cgaaggccaa ccccagtttg agcacaaaca gcgtgccagc aaaactgatc
      121 ccggtcaggc gtttaccatt gcaccactcg ccgtagtcga cggtatcgga catcattacc
      181 cactggatag gtgtcaccag ttgatgcaac acaccaatca cgaagatgaa gacaaacata
      241 gtgatgctgg cctgcatggg aacaaagaac atcgcgaggc taatcactgc cagcagggcg
      301 ttcgtccacc agaagatagt gactttacat ttccagtcgg tcagaggttt tgccagtgcg
      361 gaaccaatca ggttacccac gcaataagtg gtgagaaaag cgacaaacac ttccggcgtg
      421 cccaaaatcc atgtgacgta atacatcatc gccccaccgc gtacgcacac cgccaggata
      481 ttgaaaatgg ttagtaaacc gacaatccgc cactggtcgt tttgccagat atcacgtaaa
      541 tcttcccgca tagacgttgt tgtaggtggt gcttcaacgc gttctttagt ggtgaagaaa
      601 caaaatgcca gcatcatgaa tgccaccacg gaaaggaccg cgataccgcc ctggaaaccg
      661 agtggtttat tatcaccgcc aattaaatta accagtggca tcatcagaac agtagaaagc
      721 atgcctcccg cggtcgccag cacaaaacgc caggattgca gcgagatacg ctgagtcggg
      781 tcattggtga ttacaccacc caatgcgcag taagggatat tgacgacggt ataaagtaag
      841 gtaagtaggg tgtaagtaat tgctgcatag atcattttgc cgttcatact gagatctggc
      901 gtgctatagg ccagtacaca gacgatcccg aatggcagtg cgccaaacag tacccacgga
      961 cgaaatttac cccagcgaga gcgcgttcga tcggccaaca accccatgca aggatcggaa
     1021 atcgcatcca gtgcacgagc gaccaaaaac atggttccga caaatccggc aggaatgcca
     1081 aaaatatcgg tataaaagaa catcatatat aacattacgt tatcgaaaat aatgtggctg
     1141 gcggcgtctc ccatgccata accaattttc tctttaacgg acaacacttc actcttcatc
     1201 tgcttttcct tgatgctata ggggtgctga gaccgataac gcattttgta aaccatccct
     1261 gtgagagcga atattccgat atctggttat taatttatga ttcttgtttt atgtgatcgt
     1321 ggtagcgtta attccgctca tatatcattg taaaatatgg gttttatatg agttataatg
     1381 ctttcgtgat aatacgctgc gtgtattagg cggaaaaaac tgatctgggg gatgtagaaa
     1441 ctcaaggaag tagctataat gcgccccgcc tccatgtagc aatcgaggcg cggaagatcg
     1501 tcgtctccgg tgaggcggct ggacttcaaa tccagttggg gccgccagcg gtcccgggca
     1561 ggttcgactc ctgtgatctt ccgccagttt acctcttctg aactctccca atgtcaataa
     1621 atctcttcta atcccttgta tatcagtatt tatttgtatc ctgaggtcaa cctacttcac
     1681 cccatatcaa gtttcttttg ggggtacttt agggggtgtt atttcctatt gaaatacaag
     1741 cacccccata ataggaaaaa tgtgatacat aacaacggct tttaacaata tctttgttat
     1801 acaaggggtg tattgggggt atcaggttag ttactggcag aaatccccct caggaacatg
     1861 aattcatcaa aagccggagg atgtcatggc attaaccgat atcaaagtga aaaccgcaaa
     1921 acccaaagac aaaccctata agcttgctga tggcggcggt atgtatctac tgattaacac
     1981 taatggttct aaatactggc gcatgaagta ccgctttgct ggtaaagaaa agatgctttc
     2041 tattggcgta taccccgatg taacgctagc cgatgctcgc gagaaacgca gtgaagccag
     2101 gaaactcctc gctgctggag gcgatccagg ggaggcaaaa aaggaagaaa aaatcgcgca
     2161 gcaaatgagt ctgaaaaata cgttcgaggc tattgctcgc gaatggcacc agtcaaaagc
     2221 tgatcggtgg tcattacgct atcgtgatga aatcattgat acctttgaaa aagacatttt
     2281 tccctacatt ggcaagcgcc ctattgccga aatcaaacca atggagttac tggaagcact
     2341 gcgtaaaatg gaaaaacgtg gagctctgga gaaaatgcgc aaggtccgcc agcgctgtgg
     2401 tgaggtgttc cgctatgcaa ttgttactgg ccgggcagac tataacccag cccccgatct
     2461 tgccagtgct ttagctacac cgaaaaaagt acattttccc tttcttactg ctaatgaact
     2521 tccccacttc ctcaacgatc tggcgggtta taccggaagt ataatcacta agacagctac
     2581 tcagatcatt atgttaaccg gcgtacgaac acaagaactg cgttttgcac gctgggaaga
     2641 tatcgatttt gagacaaaat tgtgggagat cccggcagaa gtaatgaaga tgaagcgacc
     2701 tcatattgtg ccgctctctg agcaggtcat tatgctattt aagcagcttg aaccaatctc
     2761 aaaacatcat cccctagtct ttatcggtag aaatgatccc cgcaaaccaa ttagtaagga
     2821 aagcattaac caagtaattg aattacttgg atataaggga agactcacag ggcacggttt
     2881 cagacacacc atgagtacaa ttctgcatga acaaggcttt aattccgcct ggattgaaat
     2941 gcagttagct catgtggata aaaattccat caggggtacc tataatcatg ccctgtatct
     3001 cgatggccgc cgtgaaatga tgcaatggta cgctgattac attgattcac tctccagccg
     3061 ggaaagttaa gtcagaaata cttttccctg atat
//
LOCUS       DAEEKQ010000298         3045 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.304, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000298 DAEEKQ010000000
VERSION     DAEEKQ010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.304"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..172
                     /locus_tag="KLK57_004234"
     CDS             <1..172
                     /locus_tag="KLK57_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894435.1"
                     /translation="AKALMAQYRVPVVVEVILERVTNISMGSELDNVMEFEDIADNAV
                     DAPTETCFMHYE"
     gene            185..1036
                     /gene="hyi"
                     /locus_tag="KLK57_004235"
     CDS             185..1036
                     /gene="hyi"
                     /locus_tag="KLK57_004235"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HBE0894436.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAIRYARALGNKKI
                     NCLVGKTPAGFSSEQIHATLVENLRYAANMLMKEDILLLIEPINHFDIPGFHLTGTRQ
                     ALKLIDDVGCCNLKIQYDIYHMQRMEGELTNTMTQWADKIGHLQIADNPHRGEPGTGE
                     INYDYLFKVIENSDYNGWVGCEYKPQTTTEAGLRWMDPYFLNRVVATGKVMKPVLAQR
                     QKVVSPP"
     gene            955..2133
                     /gene="allB"
                     /locus_tag="KLK57_004236"
                     /pseudo
     CDS             955..2133
                     /gene="allB"
                     /locus_tag="KLK57_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
     gene            2190..>3045
                     /locus_tag="KLK57_004237"
     CDS             2190..>3045
                     /locus_tag="KLK57_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HBE0894437.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGLWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPYFKIQLPPFALSVAVMCLVLAMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATG"
BASE COUNT          695 a          711 c          836 g          803 t
ORIGIN      
        1 ggcgaaagcc ttaatggcgc aatatcgggt accggtagtc gtggaagtta ttcttgagcg
       61 tgtgaccaat atttcgatgg gcagcgaact ggataacgtc atggaatttg aagatatcgc
      121 cgataacgca gtggacgcac cgactgaaac ctgcttcatg cactatgaat aagggagata
      181 aataatgtta cgtttctctg ctaatttatc gatgttattt ggtgaatatg attttctcgc
      241 ccgttttgag aaagctgcgc agtgtggttt tcgcggcgtt gaatttatgt ttccttatga
      301 ctacgacatt gaagaattaa aacaggtgct ggcgagtaat aaactcgaac atacgctgca
      361 caatctaccg gcgggtgact gggcggcggg ggagcgcggt attgcctgta ttcctggccg
      421 tgaagaagag tttcgggatg gcgtagcagc agcgattcgt tatgcccgtg cgctggggaa
      481 taaaaaaatt aactgtctgg tcggtaaaac gccggctggt ttcagcagtg aacagattca
      541 cgcaacgctt gtagaaaacc tgcgttatgc cgcgaatatg ctgatgaaag aagatatttt
      601 attactgatt gaacctatta accattttga tattcctggt ttccatctca ccggaactcg
      661 gcaggcgctg aaattgattg atgatgttgg ttgctgcaat ttgaaaattc agtatgacat
      721 ttatcacatg cagcggatgg aaggtgaatt aaccaacacc atgactcagt gggccgataa
      781 aattggtcac ctgcaaattg ctgataatcc gcatcgcggc gaaccgggga ccggagaaat
      841 taattatgat tatctcttta aggtaatcga aaattctgac tacaacggtt gggttgggtg
      901 tgaatataaa ccccaaacca ccacggaagc cggtttacgc tggatggatc cgtactttct
      961 gaaccgggtc gtagccactg ggaaggttat gaaaccggta ctcgcgcagc ggcaaaaggt
     1021 ggtatcacca ccatgatcga aatgccgctc aaccagctgc ctgcaacggt tgaccgcgct
     1081 tcaattgaac tgaagttcga tgccgctaaa ggcaagctga ctatcgatgc ggcacaactc
     1141 ggtggcctgg tgtcttacaa catcgaccgt ctgcatgagc tggatgaagt gggcgttgtc
     1201 ggcttcaaat gcttcgttgc gacctgtggc gatcgcggta tcgacaacga cttccgtgat
     1261 gtaaacgact ggcagttctt caaaggtgcg cagaagctgg gcgaactggg gcagccggtg
     1321 ttggtgcact gcgaaaacgc gctgatctgt gacgcaccgg gcgaagaagc gaaacgtgaa
     1381 ggtcgcgtaa ctgcccatga ctatgtggct tcgcgtccgg tatttaccga agtggaagcg
     1441 attcgccgcg tgctgtatct ggcgaaagtt gccggttgcc gtctgcacgt ttgccatatc
     1501 agcagcccgg aaagtgttga agaagtgact cgtgcacgtc aggaaggtca ggatgttacc
     1561 tgtgaatcct gcccgcatta ctttgtactg gataccgatc agttcgaaga aatcggtact
     1621 ctggcgaagt gttcaccgcc gatccgcgat ctggaaaacc agaaaggcat gtgggaaaaa
     1681 ctgtttaacg gtgaaatcga ctgcctggtt tccgaccact caccatgccc tccggaaatg
     1741 aaagccggca acatcatgga agcatggggc ggtattgccg gtctgcaaaa ctgtatggac
     1801 gtgatgttcg atgaagcggt acagaaacgc ggaatgtctc tgccaatgtt cggcaaatta
     1861 atggcgacta acgcagcaga tattttcggt ctgcagcaaa aaggccgtat cgccccagga
     1921 aaagatgccg acttcgtctt cattcagccg aatagcagct atgttcttac caatgacgat
     1981 ctggaatatc gccacaaagt cagcccgtat gttggccgta ccattggcgc gcgtatcacg
     2041 aaaaccatct tacgtggtga tgtgatttac gacattgaac agggcttccc tgttgcgccg
     2101 aaaggtcaat ttatccttaa acatcagcag taatctggcc cctgcaatgc ccgtccttgc
     2161 ggcgggcatt ctccggttaa ggtgtgttta tgttcaattt tgcagtcagc cgcgaaagcc
     2221 tgttatcagg atttcagtgg tttttcttta ttttttgcaa cacggttgtg gttcctccta
     2281 cgctactttc tgcttttcag ttgccgcaaa gtagcctgct tacgctcacg caatatgctt
     2341 ttcttgctac cgcactggcc tgcttcgctc aggcgttctg cggtcatcgt cgcgctatta
     2401 tggaagggcc gggtggcctg tggtggggaa ccatccttac tatcaccctt ggcgaagcat
     2461 cgcgcgggac accgatcaac gatatcgcta ccagtctggc ggtggggatt gcactctccg
     2521 gcgtgctgac gatgttgatt ggttttagcg gattaggcca tcgcctggca cggttattta
     2581 cgccgtcggt gatggtcttg tttatgttga tgctgggcgc acagctgacc actatctttt
     2641 tcaaaggtat gctcgggctg ccgtttggca tagccgaccc atattttaaa attcagttac
     2701 caccgttcgc gctctcggtg gcggtgatgt gcctggtact ggcgatgatt atctttctgc
     2761 cgcaacgttt tgcccgttat ggcctgctgg tcggcaccat aaccggctgg ttgttgtggt
     2821 acttttgctt tccttcttcg cattcgctct ccggtgagtt gcactggcag tggttcccgc
     2881 tcggcagtgg cggtgctttg tcgccgggaa ttattctgac ggcggtgatt acaggtctgg
     2941 taaatatcag caatacctac ggtgcgattc ggggcacgga tgttttttat ccgcagcagg
     3001 gcgcggggaa tacgcgttat cgtcgcagct ttgtggcgac cggat
//
LOCUS       DAEEKQ010000299         2992 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.305, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000299 DAEEKQ010000000
VERSION     DAEEKQ010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.305"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            18..1076
                     /locus_tag="KLK57_004238"
     CDS             18..1076
                     /locus_tag="KLK57_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000035736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HBE0894438.1"
                     /translation="MAQAGIRPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTV
                     FTRYQHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACH
                     SLIPEAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFR
                     DHNITIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKS
                     SEWGWQIDPVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDH
                     LVQVNEAIADGVDIMEYTSWGPIDLVSASHSQMSKRYGFIYVDRDDNGEGSLTRTRKK
                     SFGWYAEVIKTRGLSLKK"
     gene            1162..2778
                     /gene="bglH"
                     /locus_tag="KLK57_004239"
     CDS             1162..2778
                     /gene="bglH"
                     /locus_tag="KLK57_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HBE0894439.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEQRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            2805..>2992
                     /locus_tag="KLK57_004240"
     CDS             2805..>2992
                     /locus_tag="KLK57_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894440.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVS"
BASE COUNT          838 a          632 c          744 g          778 t
ORIGIN      
        1 atcggctgtt tgatgaaatg gcgcaggcgg ggatcaggcc gctggtaacg ttatcccatt
       61 acgaaatgcc atatgggctg gtgaaaaact acggcggttg ggctaatcgg gcggtcatcg
      121 atcacttcga gcattacgcc cgcacggtct ttacccgcta ccaacataaa gtagcgttat
      181 ggctgacgtt taatgaaatc aatatgtcgt tacacgcgcc attcacgggc gtggggctgg
      241 cagaagagag tggcgaggcg gaagtttatc aggcgatcca tcatcaactg gttgccagtg
      301 cgcgggcagt taaagcctgt catagcctga tccccgaagc gaaaatcggc aatatgcttc
      361 tcggtgggct ggtttacccc ctcacctgcc agccacagga tatgttgcag gccatggaag
      421 agaaccggcg ctggatgttc tttggtgatg ttcaggcgcg tggccagtat cccggctata
      481 tgcagcgttt cttccgcgac cacaatatca ccattgagat gactgaaagt gacgcagaag
      541 atttaaaaca tactgtagat ttcatctctt ttagttatta catgactggt tgtgtttccc
      601 acgacgaaag cattaataaa aatgcgcagg gcaacatact gaatatgatc cccaatccgc
      661 atctgaaaag ttcagagtgg gggtggcaaa ttgatccggt tggattacgg gttctgttaa
      721 atacgctttg ggatcgttat caaaaaccgt tatttattgt cgagaacgga ttaggcgcaa
      781 aagacagcgt tgaagcggat ggttcgatac aggacgatta tcgaattgcc tatttaaacg
      841 atcacctggt acaggtaaat gaagcgattg ccgatggtgt ggatattatg gagtacacca
      901 gttgggggcc aattgattta gtcagtgcat ctcattcaca aatgtctaag cgctacggct
      961 ttatttatgt ggatcgtgat gataatggcg aaggaagcct cacaagaacg cgtaagaaaa
     1021 gcttcggatg gtatgcagaa gtgatcaaaa cgcgggggct gtcattaaaa aaataaccaa
     1081 taaagcacct taattatcgt cgcattcaga acagtctgga tgcgatacgt taattctttc
     1141 tttgcaccat aaagggatat tatgtttaga cgaaatctta ttacctctgc catcttatta
     1201 atggcaccgt tagccttttc tgcacaatca ttggctgaat cattaacggt ggaacaacgc
     1261 cttgagttat tagaaaaagc gttaagagaa acgcaaagcg aactcaaaaa gtataaagat
     1321 gaagagaaga aaaaatatac gccagcgacg gtgaatcgta gcgtaagtac gaatgatcaa
     1381 gggtatgccg ccaatccgtt cccgaccagt agtgccgcaa aacctgatgc tgtactggtc
     1441 aaaaatgaag agaaaaatgc cagtgagaca ggttcgattt attcttccat gactctgaaa
     1501 gatttcagta agtttgtgaa agatgaaatt ggctttagtt acaacggcta ttaccgttct
     1561 ggttggggga ccgcctctca tggttcacct aaatcatggg cgattggttc tctgggccgc
     1621 tttggtaacg aatactccgg ctggtttgat ttgcagttaa aacaacgtgt ctacaacgaa
     1681 aacggcaaac gggttgatgc cgttgtgatg atggatggta acgttggtca gcagtactct
     1741 accggctggt ttggcgataa cgccggtggc gagaacttta tgcagttctc cgatatgtac
     1801 gttaccacca aaggtttcct gccctttgcg ccagaggctg atttctgggt gggtaaacac
     1861 ggtgcgccga aaattgaaat ccagatgctg gactggaaaa cgcagcgtac tgatgccgca
     1921 gcgggtgtag gtctggaaaa ctggaaagtc ggtccgggta aaattgatat cgcgctggtt
     1981 cgcgaagatg tcgatgatta cgatcgcagc ctgcaaaaca aacagcagat taataccaat
     2041 actattgatt tacgctataa agatatcccg ttatgggata aagcgacctt aatggtgagt
     2101 ggtcgttatg tcacggcaaa cgaaagcgca tcggaaaaag ataatcagga taataacggg
     2161 tattatgact ggaaagatac ctggatgttc ggcacatctt taacgcagaa atttgataaa
     2221 ggtggcttca acgaattctc cttcctggtt gcgaataact ctatcgccag taactttggc
     2281 cgttatgctg gcgcaagtcc atttaccacc tttaatggtc gttattatgg tgatcacacc
     2341 ggcggaacag cagttcgtct gacctcgcag ggcgaagcct atatcggcga tcattttatt
     2401 gtggctaacg cgattgttta ctccttcggt aacgatattt atagctacga aacaggcgca
     2461 cactctgatt tcgaatctat tcgtgcggtt gttcgcccgg cctatatttg ggaccaatat
     2521 aaccagacag gtgttgaact gggctatttc acccagcaaa acaaagatgc gaatagtaat
     2581 aaatttaatg agtctggtta taaaaccacg ctcttccata cctttaaagt caataccagt
     2641 atgttgacct cgcgtccgga aattcgtttc tacgccacgt atatcaaagc tctggaaaac
     2701 gaactggatg gcttcacctt tgaagacaat aaagacgacc agtttgctgt cggtgcgcag
     2761 gctgaaatct ggtggtaatg gaacttaatg aaaggaatat tacgatgaat ataaaaattg
     2821 ctgctttaac gctggctatt gccagcggta tttctgctca gtgggccata gcagcggata
     2881 tgccagccag cccggcaccc actattccgg ttaaacagta tgtgactcag gtcaatgccg
     2941 ataacagcgt gacctttcgc tactttgccc ctggggcaaa aaacgtctcc tt
//
LOCUS       DAEEKQ010000300         2985 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.306, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000300 DAEEKQ010000000
VERSION     DAEEKQ010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.306"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            103..816
                     /locus_tag="KLK57_004241"
     CDS             103..816
                     /locus_tag="KLK57_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBE0894441.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSPLTASLVNELAARMPT
                     EHIYLGQSSGLIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGARILELDGR
                     QPADEILQASLRAIHAALS"
     gene            848..1606
                     /locus_tag="KLK57_004242"
     CDS             848..1606
                     /locus_tag="KLK57_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894442.1"
                     /translation="MDALQTQTVNSTTAPQPSYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCLSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSKIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            1652..>2985
                     /locus_tag="KLK57_004243"
     CDS             1652..>2985
                     /locus_tag="KLK57_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HBE0894443.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     AGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVVLSLIPTLKAFFQRHSLLYNAA"
BASE COUNT          667 a          732 c          807 g          779 t
ORIGIN      
        1 tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat tctgagcacg
       61 gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca acgtaatatg
      121 tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga tggttcaggt
      181 aaatcgcccc tcacggcaag cctggtaaat gaactggcag caagaatgcc aacagaacac
      241 atttatctcg ggcaatcgtc cgggctaatt ggcgaatgga tttcacagct ccctgttatt
      301 ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga aaaaccctca
      361 acaccgcctg gcaatattac tgctctggtt atctatctgc tttcctgctg gcgggcgtac
      421 aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac cgaccgctac
      481 ccgcaggttg aagtgccggg ttttcgcttt gatggcccgc aattggcaaa aaccacgggc
      541 ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca atggatggca
      601 tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc gtttgcgcgt
      661 aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc gcaactgact
      721 ttcaacggcg ccaggatcct tgaactggat ggtcggcaac cagccgatga aatcctgcaa
      781 gcgtcactac gtgcaattca cgccgcgctt tcctgatcac caaaatggat aggggatgat
      841 ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcgc cgcagcccag
      901 ctacattccg gggctaattg cggtggtcgg gtgcgatggc accggtaaat ccacgctgac
      961 caccgatctg gtgaaatcat tgcaacagca ctggcaaacc gagcggcgct atctggggct
     1021 gctctcgggc gaagacggcg acaaaatcaa acgattaccg ttggttggcg tctggctgga
     1081 acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat ctccggcgct
     1141 atgggcggcg gtgattatgt actgcttatc gctgcgaaga atggcgaatc tacgcaaggt
     1201 tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc aggctgaaat
     1261 ttcgggcttt tattacgacg gaccggggat tggcgtcgaa cgtgcgaccg ggaaaatcag
     1321 catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat atcgcccgga
     1381 attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc ctgaccatga
     1441 ctatgccgag ctgcaggaca aaatcggtgt catgtcgaag attggctata acggcacaaa
     1501 aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg cacagaaggc
     1561 ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct ggcgggatac
     1621 tttttactat caacgataag gtcaggctga attggcgggt tttaacatca aacattggtt
     1681 tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct ccagtaatgt
     1741 cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga tgacgcccgc
     1801 catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg attttattaa
     1861 gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca acaataatcc
     1921 gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca gcggcgcggt
     1981 ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag gtctggatga
     2041 ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg cttcctccac
     2101 gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac agcaggcgac
     2161 gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact ttggttttgc
     2221 gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt actggtggtt
     2281 tgcagcgcgc gaattacgcc gcagaaatat ccataatgcc ttcaaattga atctgtttga
     2341 gtctgcccga cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca ttgcccactc
     2401 catctggtcg gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt taggacccgc
     2461 tgccgccggg ttatttaaaa tcgccatgac attctttgac gccgccggaa cgccagcggg
     2521 tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca ccaccagacc
     2581 gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc tggtggcgct
     2641 cgcggtgttg atcgccggca agccgctcat ttcgctggtg tttggcgtta agtatctcga
     2701 agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc tgggtttccc
     2761 gcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg cgcaaaccat
     2821 cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg gtgtgctggg
     2881 agcagcgttt gcttacttcg gtgggcaatg tctggatgtg gtgctctcgt taattccaac
     2941 tttaaaggca ttttttcagc gtcattcctt gctttataac gcagc
//
LOCUS       DAEEKQ010000301         2966 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.307, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000301 DAEEKQ010000000
VERSION     DAEEKQ010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2966)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.307"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..216
                     /locus_tag="KLK57_004244"
     CDS             <1..216
                     /locus_tag="KLK57_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBE0894444.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTRVQGTVVMFSASGKK"
     gene            complement(271..369)
                     /locus_tag="KLK57_004245"
                     /pseudo
     CDS             complement(271..369)
                     /locus_tag="KLK57_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            complement(415..597)
                     /locus_tag="KLK57_004246"
     CDS             complement(415..597)
                     /locus_tag="KLK57_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0894445.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALVCAGYEQIFEDKLSGTRTGRPG
                     LKRALKRLQKGDALVV"
     gene            696..1586
                     /locus_tag="KLK57_004247"
                     /pseudo
     CDS             696..1586
                     /locus_tag="KLK57_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533802.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
     gene            1589..2122
                     /locus_tag="KLK57_004248"
     CDS             1589..2122
                     /locus_tag="KLK57_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000972171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE0894446.1"
                     /translation="MQHLKNIVAGNPKTVEQYQLTKNFDVIWLWSEDGKNWHEELKNF
                     QEDTIKLAYTVEGIIIAIDKDVSAINPEGLSVVELPDITANRRADISGNWMFKDGAVI
                     KRTYTEEEQRQQAENEKQSLLQLVRDKTQLWDSQLQLGIISDENKQKLTEWMLYAQKV
                     ESTDTSSLPVTFPEQPE"
     gene            complement(2151..2678)
                     /locus_tag="KLK57_004249"
     CDS             complement(2151..2678)
                     /locus_tag="KLK57_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001762409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE0894447.1"
                     /translation="MMHLKNIKAGNPKTPEQYQLTKKAGVVWLFCEDGKNWYEEQKNF
                     QPDTIKIAYDENNIIVAVSKDVSTINPEGLSIVEVPDITANRHADNNGKWMFLDGKVV
                     KREYTEQELQQQAESQKAALLSEAESVIQPLERAVRLNMATDEERTRLEAWERYSVLV
                     SRVDTANPEWPQKPE"
     gene            complement(2680..>2966)
                     /locus_tag="KLK57_004250"
     CDS             complement(2680..>2966)
                     /locus_tag="KLK57_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0894448.1"
                     /translation="ALGSGAQVASSGDIYGSVWENNWLSTWLHNHVVRDIRLGSIEYK
                     NVWRDYGFGDASGYVLTAAINSNADDIVDTVARRPIQKLIGGIWYNVGSV"
BASE COUNT          838 a          661 c          649 g          818 t
ORIGIN      
        1 gatggaactc tatacggagc cacaattctt ggcgatggag ttgtgcacaa tagcttcggt
       61 caaaaactga tgcgcattta caatcagaaa ggcatctttt caaacacaaa agattccgaa
      121 gaaggcttaa cacatatact ctcagagcat ttcgagaatg ttaaaaccag ggttcaaggt
      181 actgtagtaa tgttttccgc ttcagggaaa aaatagcatc caaccgcagc acgttcttgc
      241 ttaagacgtg ctgcggcata atcccaatga ttactccctg acagggttcg taggccactc
      301 aatatcaggt gcagttgatg tatcaacacg gttcagcaac acccgatact ttttctaggc
      361 ttccaccacc agcacgacaa gatgccgcat acagtgcccc agtcagtcca gttttcagac
      421 aaccagtgcg tcaccttttt gaaggcgctt taaagcacgt tttaatccag gtcggcctgt
      481 ccttgttccg cttaatttat cttcaaatat ttgttcatat cctgcacaaa caagagcgtt
      541 tcgttgcagg tctgtattct ggtcatttgt tgataccctt acatagccaa tcagcacgct
      601 gaatctcccg tccaaaagca caaatcatgc catgcaggcc agaaaccgcc attatctaaa
      661 acctcggttt acgagaactc ggcacaagcg gggagaaaat accgttactc agtacagcga
      721 atacctggac taatcgacaa acattcagcg gtggcctttc tggtgaacta tccggcaatg
      781 cttctacagc tgcaaaatta aaaactgcca ggaaaataag caatgtggct tttgatggtt
      841 cctccgatat cacattaaaa gcaagtcatg ttggtgcgtt tgccttaggg aaaacaggaa
      901 gcaccgttgc gaatgataaa gcagttggat ggaactggag tagcggagcc tataacgcaa
      961 ctattagtgg tgcatcaacg ttaattattc atttttatat gggagaagga agttgtcctg
     1021 cagctcagtt tcggattaat tataaaaatg gcggtatttt ttatcgttca gcccgtgatg
     1081 gttatggttt tgaagccgac tggtccgaat tttacaccac cagaaaacct tcagcaggag
     1141 atgttggtgc actgccgtta tctggtggtc aactgaatgg tgcactgggt atcggaacat
     1201 ccagtgctct tggcggtaat tcgattgtat tgggtgataa tgacacgggc tttaaacaaa
     1261 aaggcgatgg taatctggat gtttatgcta ataacgtcca tgttatgcgc tttgtctccg
     1321 gaagcattca aagtaataaa accataaata ttacgggacg tgttaatccc tcggattacg
     1381 gtaactttga ttcccgctat gtgagagatg tcagacttgg cacacgagtt gtttagacca
     1441 tgaagaaagg cgtgatgtat gaaaaatcag gccatgtaat cacggggctt ggtattatcg
     1501 gtgaagtcga tggtgatgat ccggcagtat tcagaccaat acaaaagtta attaacggaa
     1561 catggtataa cgtatcgcag gtataatcat gcagcattta aaaaacattg tcgcaggtaa
     1621 tccaaaaacc gttgaacaat accagctaac aaagaatttt gatgttatct ggttatggtc
     1681 cgaagatgga aaaaattggc atgaggaatt aaaaaacttt caggaggata caataaaact
     1741 ggcttacaca gttgagggaa taattattgc tatagacaag gatgtatcag caattaatcc
     1801 agaaggttta agtgtcgttg agttgcctga tattacagca aatcgccggg ctgatatttc
     1861 gggcaactgg atgttcaaag atggtgcggt gataaagcga acttataccg aggaagagca
     1921 aaggcaacaa gcggaaaatg aaaagcagag cctgctgcag ctcgtcaggg ataaaaccca
     1981 gctgtgggac tctcagctac agctgggcat catttccgat gagaataaac aaaaattaac
     2041 cgagtggatg ctctatgcgc agaaggttga atccacagac acctccagcc tgccagtaac
     2101 gtttcccgaa caaccagaat gagagaaggc ccgctatcgg gccttaattt ttattcaggc
     2161 ttttgtggcc attcaggatt tgccgtatcc acacggctga ccagaacact gtagcgttcc
     2221 catgcttcca gtcgtgtgcg ttcctcgtct gttgccatat tcagcctgac agctcgttcc
     2281 agcggctgga tgactgattc agcttcggaa agcaatgcgg ccttttgtga ttcggcctgt
     2341 tgttgcagtt cctgttctgt atattccctc tttactactt taccatccag aaacatccat
     2401 tttccgttat tgtctgcgtg acgatttgcc gtaatatcag gaacttcaac tatgcttagc
     2461 ccttcaggat taatagttga aacgtcttta ctgacagcaa cgatgatgtt gttctcatcg
     2521 taagcaattt ttatggtgtc tggctgaaaa ttcttttgtt cctcatacca gtttttgcca
     2581 tcttcgcaaa agagccatac cacgcccgct tttttagtta gctggtattg ttccggtgtt
     2641 ttcggattcc cggccttaat atttttcagg tgcatcatct taaacactcc ccacgttgta
     2701 ccatattccc ccaatcaatt tctgaattgg ccttctggca acagtgtcga caatatcatc
     2761 cgcattgctg ttaattgcgg ctgttaaaac ataacctgac gcatcgccaa agccgtagtc
     2821 gcgccatacg tttttatatt caatgctgcc aagacgaata tcccgaacga catgattatg
     2881 cagccatgtg cttaaccagt tgttttccca cactgaacca taaatatcgc cactagacgc
     2941 aacttgcgca ccacttccca gcgcaa
//
LOCUS       DAEEKQ010000302         2912 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.308, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000302 DAEEKQ010000000
VERSION     DAEEKQ010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2912
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.308"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..95
                     /locus_tag="KLK57_004251"
     CDS             <1..95
                     /locus_tag="KLK57_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0894449.1"
                     /translation="RLGRKSLSFSKSVELHDKVIGHYLNIKHYQ"
     gene            83..732
                     /locus_tag="KLK57_004252"
                     /pseudo
     CDS             83..732
                     /locus_tag="KLK57_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
     gene            729..1208
                     /locus_tag="KLK57_004253"
     CDS             729..1208
                     /locus_tag="KLK57_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HBE0894450.1"
                     /translation="MKSKLQKIILCLFLLCCIYNLWTLRPVQILYTYSDAGNSVFLVV
                     DHLPWTDSDKINWYLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDL
                     YCFPTIKSNDNCIVKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFK"
     gene            complement(1262..2212)
                     /gene="corA"
                     /locus_tag="KLK57_004254"
     CDS             complement(1262..2212)
                     /gene="corA"
                     /locus_tag="KLK57_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HBE0894451.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            2582..>2912
                     /locus_tag="KLK57_004255"
     CDS             2582..>2912
                     /locus_tag="KLK57_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894452.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLALTLLPLFAVAADDCALSDPMLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLVDMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQ"
BASE COUNT          877 a          645 c          619 g          771 t
ORIGIN      
        1 cacggctggg acggaagtcg ctgtcgttct caaaatcggt ggagctgcat gacaaagtca
       61 tcgggcatta tctgaacata aaacactatc aataagttgg agtcattacc caagttccgg
      121 aacaaaaata tcacaacgtg aatgcactga tattttattt gcagaaatga aagaactagc
      181 aaaaatgttt tccttttttg gacaatataa aacacttatc gaagatctga ttgaacattt
      241 tagatatgga aatggcagca attttcatag ccaacaatta aatttatctt ttcatgaaaa
      301 aatagatatg gttataatag tccaatacga ataatcaaag aatgtatcga aaatggcatt
      361 aattcaactc cctcgacagg ataccaacct cttatcctgc aaagcattaa aaccaaatta
      421 ctttcatcaa gactaaataa atttaatgac ttcgaggaca gttttaatgg cttaggaatc
      481 agtgttcatg atatttcagc tcaaaaaata tctcttttaa gttttcaaaa atacgctata
      541 ggatggagtg caacaattca ttttgtagca caggaccact tcggtcttga tgtaactgat
      601 atcaaaaaca agacttacag caaatataga ttttttcgca tatggttctt tttacaacgg
      661 cataaagact ttgctttcaa accttttttc accaatttta acactatcga aagaatcgaa
      721 aattatctat gaaaagcaaa ctccagaaaa ttatattatg tttatttctt ctatgctgca
      781 tctataattt atggacattg cgtcctgtac agattttgta tacatactct gatgctggca
      841 attcagtctt tctggtcgtt gatcatctgc catggactga cagcgataaa ataaattggt
      901 atctgaagca tcagaatgaa attaaaaatc aacatcccct accagaaggt agttggcata
      961 cctggtatgt cattgatatc ggtaatggct tcactgacta taaaaaatat atcgaagggc
     1021 cgtatgaaga tctctactgt ttcccgacaa ttaagagcaa tgataattgc atcgtgaaaa
     1081 attacttaat ggttattaat gaataccctt atcgtaacac tcatattggt attaacgatt
     1141 ttactgagta tcaattaaca caagaaaata aaatagagcg agttttcaat ccgcatgact
     1201 ttaaataaga caatttttag aaataagccg gataaaaaaa tagctttatc cggcactcat
     1261 attacagcca gttcttgcgc ttaaagtaca gatacggtgc caggcccgcg aggatcataa
     1321 agataatcgc gcctgggtag ccgaaactcc acttcagttc tggcataaac tcaaagttca
     1381 tcccatagct ggaagcaacg agcgtcggcg gcaggaatac cacggatacc accgagaaga
     1441 ttttgatgat gcggttctgc tcgatgttga taaaacccat cgccgcctgc atcaggaagt
     1501 tcaccttctg gaacagggat tcgttatgcg gcagcaggga ttcgatatct cgcaggattt
     1561 cacgcgcctg ctccagttgc ccacccggta aacgcgcttt acgcaccagg aagttgagcg
     1621 cgcgctgggt atccatcaga cacaggcgaa ctttccagcc gatatcttcc agttccgcca
     1681 gagtggagag cgcctcgtcg tactcatcgc cctgatgccc ttccataatc acccggctca
     1741 actgctccag gtcgctatag atattttcaa tttcatctgc caactgttcg attttggttt
     1801 cgaacagatc cagcagcagc tcgtaggcgt taccgtctac catcgactgg ctacgggcac
     1861 gcatacgata cagacgaaaa gcgggcagtt cacgctcacg cagagtaaac agacgaccat
     1921 cacggatggt aaatgccaca gtggagttac cggcgtgatc ttccgcatct tcaaagaaga
     1981 agaaggagtg aatatgcagg ccgtcgtcgt cttcaaagaa acgtgccgat gcttcgatgt
     2041 cttccagttc cgggcgggtt gccaggctct ggccaagttc agattgtacg cgcagtcgct
     2101 cgtcgtcgtc cggttcgaca agatcaatcc atactgcatt tacaaggggt tgtgactctt
     2161 cgacttccag ccgggtcagt cggttatttt ccagttgaaa tgcgctcagc atgaccggga
     2221 ctcccaatgc ttaaaatatc ggacagttcg gtgggcacac agaaacaaat tgggtttcag
     2281 accattaaac agcctgactc agcgcgacgg gaaaaattga ggtcgctgac aaccgctaag
     2341 gctatcagca aaaagggata gccttaggag ttgatcctgg atgacaggat agtgagccag
     2401 tatctactgg gtgtgtccaa ggcgaatgtc ctcttagagt gatcgtgcgc gcatgttacg
     2461 ccagcaaaat tttgccgtca acacgcaaca gaacaccaca gaacaatagt tgaacttcgc
     2521 gctttttggt actcgtcaag gtcgctaaca ggataaaaaa atccaacatc caggacctat
     2581 aatggcgcat cggctactca ttggtaaagg aatgatcacc ttgaatctca aacggatttt
     2641 tctcgccctc accttacttc ccttatttgc tgttgccgct gatgattgcg cactctcaga
     2701 tccgatgctg accgtacagg cgtataccgt taatcctcaa acagagcggg tgaaaatgta
     2761 ctggcaaaaa gccaatggcg aagcgtgggg aactttacat gctctgctgg tggatatgaa
     2821 tagtcagggt caggtgcaga tggcgatgaa cggcggcatc tatgatgaaa gctatgcgcc
     2881 gctcggtttg tacatcgaaa acggtcagca ga
//
LOCUS       DAEEKQ010000303         2904 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.309, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000303 DAEEKQ010000000
VERSION     DAEEKQ010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2904
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.309"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..504
                     /locus_tag="KLK57_004256"
                     /pseudo
     CDS             1..504
                     /locus_tag="KLK57_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785589.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
     gene            504..1094
                     /locus_tag="KLK57_004257"
     CDS             504..1094
                     /locus_tag="KLK57_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2313 domain-containing protein"
                     /protein_id="HBE0894453.1"
                     /translation="MDSLQDDYTKLLYGLMPPGPAWSDTDGVLDGLAPSLVRVHQRAD
                     ELVIEIDPGQSTELIERYEELYGLPDSCSPVGTQTLRQRQQRLEAKANVAGGINEQFF
                     LDQLEALGYTGVTIEQFQHLDASPDPEWGDRWRYFWRVTLPMDAGAQWQTCTDACNTP
                     IRTWGDTVAECVINKLCPSHTVVLFSYPDEDEDAQD"
     gene            1081..2121
                     /locus_tag="KLK57_004258"
     CDS             1081..2121
                     /locus_tag="KLK57_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HBE0894454.1"
                     /translation="MHRIDTLTAVKDKFGPGKNGFTDGNLRTGRLATWLNSAMWNAIQ
                     EEICGVIEKAGIELNKEEHDQLYKAILLLVGGAINEEALLIKNNLSDVEDRDEAVENL
                     GLKPTVDKAKNAVQRDGDTMTGELKIRGVNALRIFNEAFGLIFRRSEECLHLIPTSEG
                     QGENGDIGPLRPFTINLRTGEISMSHKVSVGGGSQVNGALGIGVQNALGGNSIVLGDN
                     DTGFKQNGDGLLDVYANSVHVLRFQSGSIQSNKAVNVTGRVTPSDYGNFDARYQQRNG
                     GVQDVRYGYEMYYPPGSNTVSWTFRSPSGHGLSGISISDTGRNSADNVNGVYYRPLQK
                     LINGTWYNVASI"
     gene            2124..2669
                     /locus_tag="KLK57_004259"
                     /pseudo
     CDS             2124..2669
                     /locus_tag="KLK57_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            2693..>2904
                     /locus_tag="KLK57_004260"
     CDS             2693..>2904
                     /locus_tag="KLK57_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBE0894455.1"
                     /translation="MSVVISGALTDGAGIPMSGCQIILKSRVNTSEVVMRTRADVVTG
                     NNGEYSFEAQVGKYCVYLKRDWRDEY"
BASE COUNT          756 a          635 c          828 g          685 t
ORIGIN      
        1 cgcagaccct gattatgtta tctgggcgaa agaagttcag ggtattaacc gtgcatggac
       61 tttcagacac tggaaaggca ctggaaaggc actggaacgg ttggcgtgat ggtggcgaca
      121 aacgatccgg aacgcccggc cccggatgaa agcgtgatta acgcagtcag ggaccacatc
      181 cttcctctgg cacctgttgc cggaagcgga ttgtatgtat tcggtgccac agaaaaagtc
      241 atcccgatga cgattgcatt atcgaaagac acaccgcaga tcaggactgc aataaaagca
      301 gagctgaatg cactgatgtt acgggatggt gtgccagagg ggcgtatgta tctgtccaga
      361 atcagtgagg ccatcagttt gtctgctggt gaagtggccc accggttaat cgtcccttca
      421 tcggatattg acctggggga gaccgagctt ccggtgctcg gcgagatcac ctggcaggct
      481 tatgacccgg caaggagtaa ataatggatt cgttacagga tgattataca aagctgctgt
      541 acggactgat gccgccgggg cctgcatggt cagataccga cggtgtactt gacggtctgg
      601 caccatcgct tgtgcgtgtt caccagcggg ctgatgaact ggtgattgaa atcgatcccg
      661 gtcagtccac agagctgatt gaacgttatg aagaattgta tggtttacct gattcctgtt
      721 cccctgtcgg aacccagaca ttacgccagc gtcagcaacg tcttgaagca aaagccaatg
      781 tggctggtgg cataaatgag cagttcttcc tggatcagct tgaggcgctg ggatataccg
      841 gcgtaacgat cgaacagttc cagcacttgg atgcaagccc cgatccggaa tggggagatc
      901 gctggcgtta tttctggcgt gtgacgttgc cgatggatgc cggtgctcag tggcagacat
      961 gcacggacgc ctgcaacaca ccgatccgga cgtgggggga tacggttgcg gagtgcgtga
     1021 ttaataaatt atgtccgtca cacaccgtcg ttttattttc ctatccagat gaggatgaag
     1081 atgcacagga ttgatacgct gactgcggta aaagataagt ttggtccggg taagaacgga
     1141 tttactgacg gaaatcttcg cacaggacgt cttgctacct ggctgaacag tgccatgtgg
     1201 aatgccattc aggaggaaat ctgtggtgtc attgagaagg ccgggataga actgaataaa
     1261 gaagaacacg accagctata taaggctata ttattgctgg tgggcggtgc aattaacgaa
     1321 gaggcattgc tgataaaaaa taacctttcg gatgtggaag acagggatga ggctgttgaa
     1381 aacctcggat taaaacccac ggtggataag gcaaaaaatg ccgttcagcg tgatggcgac
     1441 accatgaccg gagagctgaa aatccgtggt gttaatgcgc tgaggatttt caacgaagct
     1501 tttggcctga tttttcgtcg ttcggaagag tgcctgcacc ttattcccac cagtgaaggt
     1561 cagggggaaa atggcgatat tggtcccctg cgcccgttca ccattaatct gcggacgggt
     1621 gaaatatcca tgtcgcataa agtgtctgtt ggcggtggtt cgcaggtcaa tggtgcgctg
     1681 ggtatcggcg ttcagaacgc cctggggggg aattcaattg ttcttggtga taatgacacc
     1741 ggattcaaac agaatggtga tggcctgctg gatgtttatg ccaatagcgt gcatgtgttg
     1801 cgttttcaga gtggcagtat ccagagtaat aaagctgtaa acgttacagg acgggtaaca
     1861 ccgtcagact acggaaactt tgatgcccgt taccagcagc gaaatggtgg cgtgcaggat
     1921 gtgcggtatg gttatgaaat gtattacccc cccggcagta acaccgtttc gtggacattt
     1981 cgctcacctt cgggacacgg gctgtcaggg atatcgatat cggataccgg ccgtaactca
     2041 gcggataacg tcaacggcgt gtattaccga ccgctgcaaa aactgattaa tggcacctgg
     2101 tataacgtag cgagtattta acaatgttgc atttaaaaaa tattacggca ggtaatccga
     2161 aaaccgcgga ataatatcag ctgacaaaac agtatgatgt cacctggctt ttttcggaag
     2221 acggcaaaaa ctggtatgaa gaacagaaga actttgccag tgacaccata aaaatggttt
     2281 acaccggaga cgggcgcgtg gtgtgggtcg gtaaggatgt gacaggcatt gaaccccgta
     2341 acgccagtgt tattgaagtt cctgatatta ccgccaaccg ccgcattacc gcgccgggtt
     2401 actggtttta ccgcaatgat gaatttgtct ttgactacag actcaaagcg gaagatgagc
     2461 gtgatgccct tctggctcag gtcagtgccc ggacagggga atgggaagaa gacctgctgc
     2521 tggggctaat cagcgacgaa gaccgggaaa aactgaaagc gtaccgtatt tacgcgaaat
     2581 cgctgcaggc gatggatttc agcaccatca ctgataaatc ctcatataac gccattgaat
     2641 ggtccgtctc tccggaaggt tcttcctgat ttaatttatc gcgagaaaaa ctatgtctgt
     2701 agtgatatca ggtgcgctga ctgatggcgc aggcatcccc atgtccggat gccagattat
     2761 tctgaaatcc cgtgtaaaca cctcagaagt ggtgatgcgt accagggctg atgtagtgac
     2821 cggaaataac ggtgaatatt cgtttgaggc acaggtcgga aaatattgcg tgtatctgaa
     2881 acgggactgg cgcgacgagt actg
//
LOCUS       DAEEKQ010000304         2898 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.310, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000304 DAEEKQ010000000
VERSION     DAEEKQ010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2898
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.310"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..301
                     /locus_tag="KLK57_004261"
     CDS             <1..301
                     /locus_tag="KLK57_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894456.1"
                     /translation="RVELYYRFNHEEEYYPSHRFMLVVDMKTVMVFSQRAESLGLYSN
                     NRLLINIIAEHIHSDIYLTKYQQQYPDANALINTLHESGNDMVQDEKHLSDSKNR"
     gene            390..2258
                     /locus_tag="KLK57_004262"
     CDS             390..2258
                     /locus_tag="KLK57_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HBE0894457.1"
                     /translation="MIFFNDIKPTGWLKNKMEWYMNGHIGELDKLVPHLITEHKIYGR
                     DRITPRTVSAEDGVIKKQNNPDDNMMQYYWWDSETQSNWKDGYCRSAYLLDNKEWQEK
                     ASDLCLELIDTQDDDGYIGIYDSSLKFNCKAENGEFWAQATALRFVLGCYESTRNESL
                     LTSLTQAAQCIMAGYPKETSHPFVVEQGFAGHSHGLTITDVFYRLYEITNNIEYLSYC
                     LWLYEDYSAGCVSEQDLQYKNVIDPGYLLKGHGVHTYEHIRALAIAASQRPQYQTALD
                     IFLSQLKYYLTPSGAPIGDEWITGRTANATYTGYEYCSVHELFSTYVMLIKLTTNIGL
                     ADDAEWLFYNASFGMQHPILHAIMYCKTDNCYEANERKHPDSNQFNTRYKYSPTHEEA
                     AVCCVPNTARTIPAFVENMFQENNNGFTALFYGPCEFYGTFNNVAIKITQLTEYPHDL
                     SVKCLIEPESSVRFALSFRYPGWAKMMIINGVTFTKNDTQNSLIILDRTWQHHDVIDI
                     KFIADIQFNTDLCGDTYISRGPVLYAIELESDILIKKNLLNGKYFDSGYIPCSRADES
                     IQFSYADMESFSYSTSPEGKQYIEGCFYLNKTKIVRKLIPFGQTILRKVTFSNIES"
     gene            2295..>2898
                     /locus_tag="KLK57_004263"
     CDS             2295..>2898
                     /locus_tag="KLK57_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HBE0894458.1"
                     /translation="MYLTDNIIKLSNKIGQRVYTVVSEMSIEAWITKEPQPFVYRRSG
                     AYQKLTIGSDWGQLFDCAWMRVYGKRPADKFRHKLVALVDIGGEGLIVDKNGSPICGI
                     TNKASSYGVPPDKPGKWVVDLSLVSENDEVEFWIDAACNDLFGYVTNGGIITDVHIAT
                     RNQLLKSLYYDIEVLFDWINDGQKFESIHPKGITPEQFIAK"
BASE COUNT          898 a          520 c          568 g          912 t
ORIGIN      
        1 acgtgtagaa ctgtattacc gctttaacca tgaagaagaa tattaccctt ctcaccgttt
       61 tatgcttgtt gttgatatga agacagtaat ggttttttca caacgagctg aatcactggg
      121 gttatactcg aataatcgct tgcttatcaa tataattgcc gaacatatcc atagtgatat
      181 atatctaacc aagtatcagc aacaatatcc agatgccaat gcattaataa atactttgca
      241 tgaatctggc aatgatatgg tgcaggatga aaaacatttg agtgatagca aaaaccgtta
      301 atattttcct ttcattgtga tcgtctgtag gcgatcacaa tctatttgtc ttttataacg
      361 tctaaacttt tttatgcaaa ggtgtattta tgattttttt taacgatata aagcccactg
      421 gttggttaaa gaataagatg gaatggtata tgaacggtca tataggagaa ctggataaat
      481 tagttcccca tcttattacg gagcataaaa tatatggaag agatcgtata actccacgca
      541 ctgtatctgc tgaagatggc gttattaaaa aacagaataa tcccgatgat aacatgatgc
      601 aatattattg gtgggatagt gaaactcaat cgaattggaa ggatggatat tgccgcagtg
      661 cctacttgtt agataataaa gaatggcagg aaaaagcgag cgatttatgt ttggaactta
      721 tcgatacgca agatgatgat ggctacattg gaatttatga ttcatcatta aaatttaact
      781 gtaaagcaga aaacggagag ttctgggcac aagctactgc cttgcgtttt gtgcttggat
      841 gttatgagtc cacccgtaat gagagtttat taacttctct tacccaagcc gcacaatgca
      901 ttatggctgg ctaccctaaa gaaacgtcac atcctttcgt tgtggaacag ggttttgctg
      961 gacatagcca cggattaaca ataacagatg ttttctaccg cctttatgaa ataaccaata
     1021 acattgaata tctctcatat tgcttgtggc tgtatgaaga ttattccgca ggatgcgtgt
     1081 cggagcaaga tctgcaatat aaaaatgtca tagaccctgg ttaccttctt aaaggtcatg
     1141 gcgtacatac gtatgagcat attagggcat tagccatcgc agcgagccaa cggccgcagt
     1201 atcaaactgc attagatatt tttttatccc agttgaaata ttatcttaca ccatctggcg
     1261 ctcccattgg tgatgagtgg attacaggac gcacagctaa tgccacttat acaggctatg
     1321 aatattgttc tgttcatgaa ctatttagca cttatgttat gttaataaaa ctgaccacta
     1381 atattggtct ggccgatgat gctgagtggc tattctataa tgctagtttt ggcatgcagc
     1441 atcccatact tcacgcgata atgtactgta agactgataa ttgttatgaa gcaaatgaaa
     1501 ggaaacatcc tgacagtaat cagtttaata ccagatataa atactcccct actcatgagg
     1561 aggctgctgt ctgctgcgta ccgaatacag caagaaccat accggctttt gtcgaaaata
     1621 tgtttcaaga aaataacaat ggttttactg cattatttta tggcccgtgt gagttttatg
     1681 gcacatttaa taatgttgca attaaaatta cacaattaac tgaataccct catgatttaa
     1741 gcgtaaaatg tctcattgag cctgagtcat ctgttcgttt tgctctcagt ttccgttacc
     1801 ccggctgggc aaaaatgatg attattaatg gtgttacttt tactaaaaat gatactcaaa
     1861 actcacttat tattttagat agaacatggc aacatcatga tgtgattgac attaaattta
     1921 tagctgatat acagtttaat actgacttat gtggtgacac ttatatttcc agaggtcccg
     1981 ttttatatgc aattgagctt gagagcgata ttttaatcaa aaaaaacctg ctgaacggga
     2041 aatattttga ctcaggatac ataccctgta gtagagctga tgaatctatc cagttctctt
     2101 atgctgatat ggaatctttt tcatattcaa cctcaccaga gggcaaacaa tacattgaag
     2161 gctgttttta tttaaacaag actaaaatag tcagaaaact tataccattt ggtcaaacca
     2221 ttcttcgcaa agtaactttc tccaatatag agagttaata ttttcgagtg ttataattaa
     2281 ttaaagggat ttctatgtac cttactgata atattatcaa actgtcgaat aaaataggtc
     2341 agcgtgttta tactgttgtc tctgaaatgt cgattgaagc atggataaca aaagaaccgc
     2401 agccttttgt ttatcgccgt agcggcgctt accagaaact caccattggc tcagactggg
     2461 gtcaactgtt tgactgtgcc tggatgcgag tttatggtaa acgtccagca gataaattta
     2521 gacacaaact cgtcgctctc gttgatatcg gtggcgaagg tcttattgtg gataagaatg
     2581 gttcacccat ctgcggcatc acgaataaag catcatctta tggcgtaccg ccagataaac
     2641 caggtaaatg ggtggttgac ctttctctcg ttagtgaaaa tgacgaggtt gagttttgga
     2701 ttgatgctgc ctgcaacgat ttatttggct atgttactaa tggagggatt attactgatg
     2761 ttcatatcgc gacacgtaat caactactga aatcccttta ttatgatatt gaggtgcttt
     2821 tcgactggat taatgatggt cagaagtttg aatccataca tcctaaaggt atcaccccag
     2881 aacaatttat tgccaaaa
//
LOCUS       DAEEKQ010000305         2878 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.311, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000305 DAEEKQ010000000
VERSION     DAEEKQ010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.311"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..954)
                     /gene="curA"
                     /locus_tag="KLK57_004264"
     CDS             complement(<1..954)
                     /gene="curA"
                     /locus_tag="KLK57_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HBE0894459.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPSDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGL"
     gene            1135..1653
                     /gene="mddA"
                     /locus_tag="KLK57_004265"
     CDS             1135..1653
                     /gene="mddA"
                     /locus_tag="KLK57_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HBE0894460.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDGVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHHSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            1650..2099
                     /locus_tag="KLK57_004266"
     CDS             1650..2099
                     /locus_tag="KLK57_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HBE0894461.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(2100..2333)
                     /locus_tag="KLK57_004267"
     CDS             complement(2100..2333)
                     /locus_tag="KLK57_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HBE0894462.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="KLK57_004268"
     CDS             complement(2419..2592)
                     /gene="ortT"
                     /locus_tag="KLK57_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HBE0894463.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            2787..>2878
                     /gene="yncL"
                     /locus_tag="KLK57_004269"
     CDS             2787..>2878
                     /gene="yncL"
                     /locus_tag="KLK57_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HBE0894464.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWF"
BASE COUNT          691 a          738 c          692 g          757 t
ORIGIN      
        1 taaaccatca gtaatatctt cgtggtagtg gattttatcc tctttcaccc attgccccat
       61 ctccttctga aactcatgga tgcggtgacc ataatcctga gcgataataa acccttgcaa
      121 gcgaatacgt tttttcagca ccgttgccat caacaggggt aaacgatccg gaccgggtgg
      181 tagctctgta gcgttatagc tgctcactaa gccacaaacg ggaatgcgcg cagatgtatt
      241 aagtaatggt agcactgcat caaatacctt accgcccacg ttttcataat agatatcaat
      301 accttttggg cacgctttca ccagttgttc ggcaaaatca tccgcgtggt gatcaagaca
      361 aacatcgaag cccagcacct cgatagcatg gcggcatttt tccgcgccac cggcaacccc
      421 caccaccctg caacctttaa gtttgccgat ttgccccacc gttgctccca caggacctgt
      481 cgccgcagct accaccaacg tttcgccctc tttaggctga ccgatatcca gcagccccat
      541 ataagcggta aagcctggca tccccagcac acccagcgac cacgatggat tttgcggatg
      601 atcgccaagt ttcaccagat catcaccact ggatatgtca tagtcctgcc atccactgta
      661 gcccagcacc cagtcgctag gctgataatc aggatgattc gactccacaa cacggctcac
      721 cgtgccgccg accatcacgc cgccaatatc aacaggcggt gaataagatg gctcatcgct
      781 catacgtcca cgcatatacg ggtccaggga caaataaact gtgcgcagta acacctgtcc
      841 ttcacccggt gtggcgacat catcttcttc aagacggaaa ttctccggaa ccggtgcgcc
      901 atgtggacgc gaggccagaa cccaacgtcg attacgctgc ttttgttgcc ccataatgct
      961 ctcctgtgct tttggcatga atccatcaat agtagcaggc tcacgggatt ggttttgttt
     1021 gcagccgtca catcccctct gcaacacgca ataatttcat cgaggaatcc gttgcttcgg
     1081 gctgttttcg atagtattga taacatcatc atcattattt tatcgaggcc agacatgtcc
     1141 atccgttttg cccgcaaagc cgattgtgct gccattgcgg aaatttataa ccacgccgtg
     1201 ttgtatacgg cggctatctg gaatgaccaa acggttgatg cagataaccg cattgcctgg
     1261 tttgaagcgc ggaccatagc aggttatccg gtgctggtaa gtgaagaaga tggcgtagtg
     1321 acgggatatg cctcgtttgg cgactggcgt agtttcgatg gttttcgcca taccgtggaa
     1381 cattcggttt atgtccatcc cgatcatcag ggcaaaggtc tggggcgtaa attgttaagc
     1441 cgattgattg atgaagcgcg ggattgcggg aagcatgtca tggtcgccgg gatcgaatcg
     1501 caaaatcagg cctcgctgca tctgcaccat tcgctgggat ttgtcgtcac cgcacaaatg
     1561 ccgcaggtgg gcactaaatt tgggcgctgg ctggatctga catttatgca gttgcaactc
     1621 gacgaacgca ctgaaccgga cgcgattgga tgaatcagtc gctcaccctt gcttttttga
     1681 ttgccgccgg aattggtctg gtagtccaga acacgctgat ggtgcgcatc acccagacct
     1741 cctctaccat tctcatcgcc atgttgctga actcactggt ggggattgtg ctctttgttt
     1801 ctattttatg gtttaaacaa ggcatggcgg ggtttggcga actggtgtcc agcgtgcgct
     1861 ggtggacact tatacccggc ttgctgggat cgttttttgt cttcgccagt atcagcggat
     1921 atcagaatgt gggagcggcg acaactattg cagtactggt cgccagccag ctaattggcg
     1981 gtctgatgct ggatatcttc cgcagtcatg gcgtaccact acgggcgttg ttcggcccaa
     2041 tttgtggcgc aattttactt gtggttggcg cctggttggt agccagacgc tcattttaat
     2101 cagaggatgg tgccgccttt ggttaactgt tcgtggcgcg cttccatatc ttctttatgc
     2161 ttgcgaccgt gatgagcaat agcggtgcgc aaacgctgct gctgggtgta gcgatcttcc
     2221 cgacttaact ccgcgtcatc gctcagggcg attaataatt cgttcatatg ggcaatgacg
     2281 ctctcttcaa tcgcggcatc cacgcgcgcg atgacttcat ccagatgcga catactttct
     2341 ccttaaccac ttgtgaaatt tatcgattca tccaaatgat ataagtctgc gtggtgccgt
     2401 caagccagtg agtaagattt aaaacagtga aaacaacagc gccaccggga aactcatcgg
     2461 ccatgttgcc cccaccagaa aggcgcttaa gaaacggatg cgtttcttat cgtgagaaag
     2521 aaaccaggtg ataagaaatg cgattgctgc cataaccgca taaaaaacaa gcatatgttg
     2581 atagagagac atgtacgggc gtccagccag atgaataaaa cgcgcgcaaa tatgcgtcat
     2641 tttttgacgt gcatcaagtt ttttcaccag tttgagaaaa aatcatcaaa cagcaacttg
     2701 cccttatcca tcttcagaat gagtattatt gcggattttc ttaacccgta ctatacacat
     2761 aggggcaatg ataaaaggtg gcaaaaatga atgtttccag tagaactgta gtactgataa
     2821 atgtctttgc tgctgttggt ttgtttactc ttatctctat gagatttggc tggtttat
//
LOCUS       DAEEKQ010000306         2835 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.312, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000306 DAEEKQ010000000
VERSION     DAEEKQ010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.312"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1497)
                     /locus_tag="KLK57_004270"
     CDS             complement(<1..1497)
                     /locus_tag="KLK57_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBE0894465.1"
                     /translation="MSGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPGGIT
                     YANPVNPVLGAKVLPGETDLALPCPLPFILFRAYSSYRTRTPAPVGVFGPGWKAPFDI
                     RLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQ
                     VLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGR
                     TLAFHRAAEGDVAGAVTGVMDGAGRRFHLVLTTQAQRAEEARKPHTASLSSPDSPCPL
                     SAPSFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGELRAVY
                     DRSGMQVRGFTYDAEHAGRMVAHHYAGRPESCYRYDDTGRVTEQVNPEGLDYRFEYGE
                     SRVIITDSLNRREVLYTEGEGGLKRVVKKEHADGSITRSEYDEAGRLKAQTDAAGRRT
                     EYRLHMASGKLTSVILPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDE"
     gene            complement(1755..2723)
                     /locus_tag="KLK57_004271"
                     /pseudo
     CDS             complement(1755..2723)
                     /locus_tag="KLK57_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1-like element ISEc1 family transposase"
BASE COUNT          604 a          865 c          638 g          728 t
ORIGIN      
        1 ctcatcatac tcccggctgc tgcgaagccc gtccgggtac gtcaccgacg tcacctgccg
       61 ctggctgtta tagccatacc gtaccgtcct gccgtccggc agtatcactg aagtcagctt
      121 tccagacgcc atatgcagtc ggtactccgt ccgccgtccc gccgcatccg tctgcgcctt
      181 cagcctcccc gcctcatcat actcgctgcg ggtgatgctc ccgtcggcat gctccttctt
      241 caccacccgc ttcaggccac cctcgccttc cgtgtacagc acctcccgcc ggttcaggct
      301 gtccgtgatg attacccggc tctctccgta ctcaaagcgg tagtccagcc cctccgggtt
      361 gacctgctcc gtcacccgac cggtatcatc ataccggtag cagctctccg gcctccccgc
      421 ataatggtgc gccaccatcc gcccggcgtg ctccgcatca taagtaaacc cgcgcacctg
      481 catcccgctg cggtcataca ccgcccgcag ttcaccaccg gccgtgtacg tgtagcgcgc
      541 cagcggcgca gtcggctgtt catccgggta tgccgggtcg tgtgtcagcc ataccgcctc
      601 cagccggata ccgttatcgg caccgtactc tgtaccggcg ggcagcgtgt cggggaagga
      661 aggagctgag agagggcagg gactgtcagg ggaagataac gaagcggtgt gcggtttacg
      721 ggcctcttcc gcccgctgcg cctgtgtggt cagcaccagg tgaaaacggc ggcccgcgcc
      781 gtccatcacc cccgtcaccg cgcccgccac gtcaccctcc gctgcccggt gaaaggccag
      841 cgtccgcccg aagccgtcca ccacgcccgt cagcacccgg tatgcgggcg gctccggcgg
      901 cagcacctcg tccgcccccg gcacccgctc cggccagctg agtatccacc acggcccctg
      961 aaggctgttt gtcgccaggt acacatgcgg actcaggcga acatcttccg gcagcacctg
     1021 ccagagcgcg cttagcggct gcgaactgtg ctgcgccgct actccgcccc gcgccagcca
     1081 gagtgactcg ctgcggctgt agcttatctc gccgggaaac agcggctcaa agtgaatgct
     1141 cctgccgccg ctgtcgttga gtatcaggcc ttcatcgcgt atctgtaagc ggatatcgaa
     1201 gggcgctttc cagccgggac caaacacccc caccggcgcg ggtgttctgg tccggtagct
     1261 gctgtaggcg cggaaaagaa taaacggcag cgggcagggc agcgcaaggt ccgtctcgcc
     1321 cggcagcacc ttcgcaccca gcaccgggtt caccgggtta gcataggtaa tccctcccgg
     1381 acacaccgaa caggccactc ccgtcggcgc acctatcagc acacccgctg aaccctgcac
     1441 aatatccagt cccttgcggg tcatgtcgcc ctgccgcgcc gccggttttc cgctcatggt
     1501 ttattcctct tcatctgaat tgatttacat taggttggtt attttctcat acccacaact
     1561 atattttctc tctgaattta ttacgtgcaa gacaatcgca gcactctaac taacttcgat
     1621 gcaatttgat gttcagaaaa atccctcatt agattattat catgaaatac agctaatcat
     1681 ctttagctta tatatttctc aatgtattct tttgaaagga tccgtgccct ttcaaactct
     1741 tcagcagggc aagattacga aagcccgctc cccgcaagga ctgacgccag atagtttctg
     1801 tccatggctg cttttcgcat cttacgtctt aaccctgcct tgaatacctt atcattcgtc
     1861 aaaatattaa tagcaatgtg ccgtatccct gaaaataatt ctgctgcatt tcctcttctt
     1921 attttgcagt cgtcttcatt cattaccacg tccagacgcc agtgcagctt attctccacg
     1981 tgccagtggt ttcggattgc tgtggcgaac ttttctgcag ttaaatcagc agaactgata
     2041 taatatctga ccgtcatttc tggctctttc ttttgttctg ctattattga ccgaaaggag
     2101 actgccacgc ataatttctt cagtcctttc cattcaaacg tgaaatcaat aagttcatca
     2161 gggacatcgc aaacaatatg aagacggatt tcttctctgc cgtgactctt ttcactgatt
     2221 gcgtaactgt catgctctgg attatttaat tctttcagcg gaaatttttc ctcaaaggct
     2281 ttatttagcc gcccctggtt tccttttaca gcgaataaat aatcacctcc ctgtttttgt
     2341 atcttctctg caatatcttt ctggcaaccc atcgcatcag ttgtgatgat ttttccttta
     2401 atatccagca tgttaagaag ttcaggaatg gctgtaatct cattggattt ctcatccgtc
     2461 ttgatctgcc cgaggaccag actgtacatt gttgagaacg cactaatgac atgaatcgct
     2521 cccctgcgac gactcttatc ataagagtgc cggagcgttt ttccatcaat tgcaatgacg
     2581 tctttatcat ctgaagaatg gcagtcacgc atccagttaa taaagcactc atgaaatttt
     2641 gcaggactga tacaggatac aactctggca atggtatcgt gaacaggaat gccattttca
     2701 aaatcaccat attgcttcaa aaaatgttcc atcaattttt taatttccat tttgttctcc
     2761 ttaattatgt aaggagtatt tgattatgta taagcaataa aaaaacagct tcaggtaatg
     2821 aggaatatct caatt
//
LOCUS       DAEEKQ010000307         2813 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.313, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000307 DAEEKQ010000000
VERSION     DAEEKQ010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.313"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..989
                     /locus_tag="KLK57_004272"
                     /pseudo
     CDS             1..989
                     /locus_tag="KLK57_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            1005..1754
                     /locus_tag="KLK57_004273"
     CDS             1005..1754
                     /locus_tag="KLK57_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HBE0894466.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRDFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            1756..>2813
                     /locus_tag="KLK57_004274"
     CDS             1756..>2813
                     /locus_tag="KLK57_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894467.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKIT"
BASE COUNT          885 a          566 c          585 g          777 t
ORIGIN      
        1 aggttaaata catacgtaag tttggctcgt aatacttact gggacgccag cagtaatgtg
       61 aattattcat tatcacttag ccgcgatttt gatatcgggc cattaaaaaa cgtctccgct
      121 tctctaacat ttagccgaat aaactgggaa gaagacaacc aggatcaact gtacctaaat
      181 atttcgattc cctggggaac cagtagaaca ttgagctatg gtatgcaacg aaatcaggat
      241 aacaagattt cgcatactgc ttcgtggtat gactcttccg atcgaaataa ttcctggagc
      301 gtttctgctt caggcgacaa tgatgaattc aaagatatga aagcgtcact acgcgccagt
      361 tatcagcata ataccgagaa cggtcgtctc tatctctccg ggacatcaca gcgagacagt
      421 tattattctc tgaatgccag ttggaatggt tcattcacgg cgactcgtca cggagccgct
      481 ttccatgact atagtggtag tgctgactcg cgttttatga tcgacgcaga tggtgcagaa
      541 gatattccgt tgaacaataa acgcgcggta actaatcggt atggcatcgg agttattcca
      601 tcagtcagca gttacataac aacatcatta agcgttgata cccgaaatct tccagaaaat
      661 gtggatatcg aaaactcggt tatcaccacc accttaaccg aaggtgctat tggctacgcc
      721 aaacttgata ccccaagggc tatcaaatca tgggggttat tcgcctggca gatggtagtc
      781 acccaccact ggggattagc gtaaaagata aaaccagcca taaagaatta ggactggttg
      841 ctgatggcgg ctttgtatac ctcaacggca ttcaggatga cagcaaactt actttacgct
      901 ggggtgataa atcctgtttt attcaaccac ccaatagcag caacttaacc accggaacgg
      961 ttattttacc gtgtattagc caaaattaat tcaggatgaa actaatgcgc tacttaaaca
     1021 caaagaatct cattgcagca ggagtattac tcgcctgcat gagcagcatt gcctggggtg
     1081 ccatcatccc ggaccgtacc cgtatcataa tgaatgagtc agataaaggg gaagcattaa
     1141 aacttactaa tcaaagcaaa aatctaccct atctggcgca aacatggata gaagatacga
     1201 aaggcaataa atcacgtgac tttattgtca cggttccccc tatggtacgt ttaaatccca
     1261 gtgagcaaat tcaaataaga atgataagtc aggaaaaaat tgctcaactg cctaatgaca
     1321 gagaaacgct cttctatttt aacgtgcgag aaataccgcc aaaaacagat aaaaaaaatg
     1381 tgatgcaagt cactatgcag cacgcattga aactattctg gcgaccaaaa gctattgaat
     1441 tagaagatga tggggtaatg tcttacgaaa aagttgaaat catccgtaga aatgatggtt
     1501 cgatccgctt taacaataag atgccttatc acgttacgct gggttatatc ggaacaaacg
     1561 gaatcacaat gttgccacaa acgcaaagtt taatggtaac cccttatagc catgcagata
     1621 cgcagtttaa aaatgttccg tcagcttttc aggttggtta tatcaatgat tttggtggat
     1681 taagttttta tgaaataaac tgcccgacgg ttaataactc ttgtaatgtt tccgtagcca
     1741 aacgagataa ataatatgcg ctacttatta attgtaatta tatttattat aggttttagt
     1801 gcattacctg tatgggcaat ggactgctat gctgaacatg aagggggaaa taccgtagtc
     1861 attggttacg taccaagaat ttctatcccc agtaatggta aaaaaggtga taagatctgg
     1921 caaagcagtg agtattttat gaatgtcttc tgtaataatg cattacctgc cccatcacct
     1981 ggagaagaat acccatcagc ctggactaat ataatgatgt ttttaccagg cggtcaggac
     2041 ttttataatc agaactccta tatttttggc gtaacataca atggagttga ttatgattcg
     2101 actgccccta atgctatcgc agcaccagaa tgcattgata taaaaggagc gggtaccttt
     2161 aataatcact ataaaaaccc cgctgtctgt agtggaggtc ctgagcctca attatcagta
     2221 acctttccag ctcgcgtaca actctatatt aaattagcaa aaaatgccaa tagagttaat
     2281 aaaaacctgg tgttacctga tgaatatatc gctctagagt ttaaaggtat gagtggcgca
     2341 ggagctatag aagtagacaa aaatttgacc ttcaggattc gtggattaaa taatattcat
     2401 gtccttgact gctttgttaa tgtcgcactg gaaccagctg atggcgttgt tgactttggt
     2461 aaaataaatt cccgaacaat taaaaatacc agcgttagtg agacgtttag tgtagtcatg
     2521 acaaaagatc ctggtgcggc ctgtactgag caattcaata ttttagggag ctttttcact
     2581 acggatattt tgagtgatta tagccatctg gatatgggta atggactgct attgaagata
     2641 tttcataaag atggaacagc aacggaattt aaccgcttct cacaatttgc ttctttttca
     2701 tcgtctagtg caccttcggt caccgcacca ttcagggcag aacttagtgc gaacccggca
     2761 gagactgttg ttgagggacc gtttagtaaa gacgtaatcc tcaaaatcac cta
//
LOCUS       DAEEKQ010000308         2739 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.314, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000308 DAEEKQ010000000
VERSION     DAEEKQ010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.314"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="KLK57_004275"
     CDS             complement(<1..1007)
                     /gene="shiA"
                     /locus_tag="KLK57_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HBE0894468.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRVIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGALIGTLSAFPFF
                     MA"
     gene            1322..2374
                     /locus_tag="KLK57_004276"
     CDS             1322..2374
                     /locus_tag="KLK57_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HBE0894469.1"
                     /translation="MFLASLLRRIAFSYYDYKAYNFNIEKTDFVVIHIPDQIGDAMAI
                     FPVIRALELHKIKHLLIVTSTINLEVFNALKLEQTKLTLVTMTMQDHATLKEIKDLAK
                     NITQQYGTPDLCIEGMRKKNLKTMLFISQLKAKTNFQVVGITMNCYSPLCKNASRMDQ
                     KLRAPVPMTWAFMMREAGFPAVRPIYELPLSEDVLDEVREEMRSLGSYIAFNLEGSSQ
                     ERTFSLSIAENLIAKIQSETDIPIVIVHGPKGEDKARALVDCYNNVYRLSLPPSIKRS
                     AAIIKDAYIAITPDTSILHMASAYNTPVVAIYADYKTRWPAMADVSESVVVGQKIDNI
                     SLDEFAKALKSVLARI"
BASE COUNT          849 a          592 c          616 g          682 t
ORIGIN      
        1 gccataaaga aaggaaatgc gctcaacgtt ccgattaacg cacctgtgat ataaacccta
       61 cggcgaccaa aacgatcggc aagccaggca aaacagggaa ttgtcaggca gcttaatcca
      121 cctaccagca aaccaatatt aaggaaaagt tcgcgcggta gccccatatt ctgggttgaa
      181 taattaagtg caaaggcagt aacgatgtac atcgtcagca attcgcacag tcgtagcgca
      241 ataatcttca ggaaagcacc gggatgtcgt aacagcgctt cgataaccgg gatgcgtttt
      301 ttcgcggcag cttgataatg ttgctgttgt tcaaattccg cggactcctc catgccattg
      361 cgcacccaca atgctcccag taccagtacg atgctaaaca ggaaaggaat gcgccagccc
      421 cagcttaaaa actgttcgtc agtcgtcatc atactgatca atgaaaccag tccggttgaa
      481 agcagtaaac ctacaccgta gccaacttgt acaccgctac tgtaaaaggc ttttttattt
      541 ttcggtgcac tttcaacgga aagcaacgcc gcgcctcccc attcgccgcc gacggcaaat
      601 ccctgaatga cacgcagtgt caccagtaaa ataggtgccc accacccaat ggtcgagaat
      661 gaaggaagaa taccaatcaa ggctgtcgcg atgcccatca tccagacggt cagcattaac
      721 atgcgcttac gtcccagtcg gtcgccaaag tgaccgaaaa tgacaccgcc gagcggacgg
      781 aaaaggaatc cgacgccaaa ggtagcaaat gcggcgagcg ttcccatcgc cgggcttact
      841 tgcgggaaaa actcgcgatt aaacaccagt gcggcggtga tgccatagag taaaaaatca
      901 taccagtcga cgacggcacc agcgaagctg cctaacgcag ctcgtcgggc gcgacttaac
      961 gaaagcgtcc cttcatcggg acgagtggag atgagcgtgg agtccataat gatcctgttt
     1021 gtactgtctt tttataatta gtatggaaaa ggtaactcac attgccgtcg atctataaac
     1081 gaaccttatg agactacctc gacagagcgc atctgcaaat gattttatgg ctgattagcg
     1141 gcaacatgaa aaattcgatg gaagatgaaa gcgcaggagt tgctggtgtt gtttaaggat
     1201 taagcagagg acccacaggt tagagatatg aaacataaaa gtccgcatat gcacaaaatg
     1261 gcaccctgat gagtctgata gactgcattg cattataacc agtagggagg cgaggttagg
     1321 tatgttttta gcatcattgt tgagacgtat tgcatttagt tactacgatt ataaagctta
     1381 taatttcaat attgaaaaaa cagactttgt tgtcatccat attcccgatc agattggcga
     1441 tgctatggcc atctttcctg ttattcgggc gcttgaattg cataaaatta agcatctttt
     1501 aattgtaacg tcgacaatta atttagaagt ctttaatgcg cttaaacttg aacagactaa
     1561 attaacatta gtcacaatga ctatgcagga tcacgcaaca ttaaaagaaa taaaagattt
     1621 agcaaagaac ataacacagc aatacggtac gccggatctt tgtattgagg ggatgcgtaa
     1681 aaagaacctg aaaacgatgt tatttatcag tcagttgaaa gcaaaaacga attttcaggt
     1741 tgttggtata accatgaatt gctactcccc tttgtgcaag aacgcgtccc gtatggatca
     1801 gaaactccgg gctcccgtac ctatgacatg ggcatttatg atgcgtgagg cgggttttcc
     1861 agcagtcagg ccaatatatg aattgccact aagtgaggat gtactcgatg aggtgcgcga
     1921 ggaaatgcga tcgttaggat cttacattgc gttcaattta gaaggtagct cgcaggaacg
     1981 tacattttca ttatcgattg cagaaaatct aatagcaaaa attcaaagtg aaacagatat
     2041 accaatagtg atcgttcatg gacccaaagg tgaagataaa gccagggcat tagtggattg
     2101 ttataataat gtctaccgtt tatccttacc accctcgatt aaacgttcag cagcaatcat
     2161 aaaagatgct tatatcgcaa taactcctga cacctcaata ttacatatgg caagtgccta
     2221 taatacccct gttgttgcaa tttatgctga ttacaaaacg cgatggcccg caatggccga
     2281 tgtctcggag tcagtcgtcg ttgggcaaaa aattgacaat ataagtctgg atgaattcgc
     2341 aaaggcatta aaaagtgttt tggcgagaat atgagacagt acttatgcat agtaatgccg
     2401 atctgttatg aacggtggtg tcatcaatac tctgaggctg gtcaatcatc acagaaaacc
     2461 agcctttgaa tcaaagcagt actcaccgga tacgatcata gtcacatcga acgcaattct
     2521 ggcggtgccg catttttatt tattcatttt ctgtgagaga agggcaagct ctatagaggg
     2581 aatgtaaaaa agagagctcc gaatggagca ctctttaaga aattataacg gaaaatcaga
     2641 ggtttttata acatgtcgca taagcaaacc caagcttttc aaatacgctt tgatcacctg
     2701 ttgccaggga aactacgtat tgttcgcttg agtttgcgg
//
LOCUS       DAEEKQ010000309         2726 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.315, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000309 DAEEKQ010000000
VERSION     DAEEKQ010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2726
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.315"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(423..680)
                     /locus_tag="KLK57_004277"
     CDS             complement(423..680)
                     /locus_tag="KLK57_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894470.1"
                     /translation="MSDHVIECASRAGRDFSEFMKGEKGMMEALASVDEFGEQLRLNG
                     CVNHHFVSYMMRNSIMQAFMDMAKAERKEERRRKRAEAKAK"
     gene            complement(677..1174)
                     /locus_tag="KLK57_004278"
     CDS             complement(677..1174)
                     /locus_tag="KLK57_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001496757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KilA-N domain-containing protein"
                     /protein_id="HBE0894471.1"
                     /translation="MKSLTLFNQPIRIGEDGMICLTDMWKASGKSESESPYHYLRNKQ
                     TKEFLAELEKNHESVVFTERGVHGGTYGGKFVAYDYAAWLNPGFKYAAYKVLDDYFTG
                     ELQHRNSLSAQLNMKCHEFDQKKDMASFCGQGLAAWRYTKPVLVAEINSLANQLQITI
                     PGLPG"
     gene            complement(1376..1834)
                     /locus_tag="KLK57_004279"
     CDS             complement(1376..1834)
                     /locus_tag="KLK57_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009453323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein"
                     /protein_id="HBE0894472.1"
                     /translation="MSRVTAIISALVICIIVCLSLAVNHYRDNAIAYKEQRDNKASEL
                     EKANATIADMRKRQRDVAELDARYIKELADANATIESLRADVSAGRKRLQVAATCAKS
                     TTGASGMGDGESPGLTADAELNYYRLRSGIDKITAQVNYLQEYIRTQCLK"
     gene            complement(1831..2328)
                     /locus_tag="KLK57_004280"
     CDS             complement(1831..2328)
                     /locus_tag="KLK57_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HBE0894473.1"
                     /translation="MPPSLRKAVAAAIGGGAIAIASVLITGPGGNDGLEGVRHNPYKD
                     IVGVWTVCYGHTGKDIIPGKTYTEAECKALLNKDLATVARQINPYIKVDIPETTRCAL
                     YSFVYNVGAGNFRTSTLLRKINQGDIKGACDQLRRWTYAGGNQWKGLMTRREIEREVC
                     LWGKQ"
     gene            complement(2328..2543)
                     /gene="essD"
                     /locus_tag="KLK57_004281"
     CDS             complement(2328..2543)
                     /gene="essD"
                     /locus_tag="KLK57_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HBE0894474.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGG"
BASE COUNT          648 a          707 c          620 g          751 t
ORIGIN      
        1 atacagcgca tgatgtgtca ggcttgaata cctttatcct ttaaaaggga tatcagttaa
       61 gttatcccgt gtagggtata agccattatc aaagccactc tgtagggaat ggcttttgtg
      121 atggcatcac ttactcttta cgctgctatc ccactcatcc cggaattttg atgggttatt
      181 gaaaccttct gctgacataa caactccttc aatgtttggc tgaaattggg atgtctttcc
      241 atcagtccgc caccacaaag aatctttttt gccataaggc aggaggttca tctttcagtg
      301 gctgccggtg ttatttcccc acttactggc ttgggttgtt tcgtggtact gccgttaact
      361 ggtggcccag aataaattcc ggtttcatta tcaagcccac ccgtaaatgg gctttgtaat
      421 ggctacttcg cttttgcttc cgctcgctta cgccggcgct cttctttcct ctcggctttt
      481 gccatgtcca tgaatgcctg catgatcgag ttccgcatca tgtagctaac aaagtgatga
      541 ttgacacagc cgttgaggcg cagctgctcg ccaaactcat ccaccgaggc caatgcttcc
      601 atcatgccct tctcgccttt catgaactct gagaagtcgc gccccgctct ggaggcgcat
      661 tcaataacat gatcactcat cccggaagcc cggggatcgt aatctgcagc tggttagcca
      721 gggagttaat ctcagcgacc aacactggct tcgtatagcg ccatgctgcc agcccttgtc
      781 cgcagaagct cgccatatct ttcttctggt caaattcatg acatttcatg ttgagctgcg
      841 cacttaagct gttgcgatgc tgaagttctc cggtgaagta gtcatcgagg actttatagg
      901 ccgcgtactt gaacccgggg tttaaccaag ccgcataatc gtaagcaaca aacttcccgc
      961 catatgttcc accgtgtaca ccgcgctcag taaaaaccac agattcgtgg tttttctcca
     1021 gctcggctaa gaactctttg gtctgcttgt ttcgcaggta gtggtaaggc gattcagatt
     1081 cacttttacc actggctttc cacatatcag tgaggcagat catgccatct tcaccgatac
     1141 gaattggttg attgaagagg gttaatgatt tcatagcgtg tacctactct ttgaaatgaa
     1201 cctttgccgc acaggaaacc agcccaccga ggctcgccag cactaactgg tatcctcaaa
     1261 ggcccattcc aaaggggcag gttcggtgta aaaaacatgc gttgcggtac gcatttattg
     1321 caaaaagccc cgcatcgcga ggctcattaa atggactttg tgatttgcaa aaaaattatt
     1381 tcaggcattg cgtcctgatg tattcctgca ggtagttaac ctgcgcggtt atcttgtcga
     1441 ttccacttcg gagacggtaa taattgagtt cagcatctgc tgtaagtcct gggctttctc
     1501 catcgcccat gccgctggct ccggtcgttg actttgcaca ggtggcggcg acttgcaggc
     1561 gcttacgccc agcagaaaca tcagcacgga gactttcgat agtcgcgtta gcatcagcaa
     1621 gctcctttat gtatcttgcg tcgagttctg ctacatcacg ttgacgcttc cgcatgtcag
     1681 cgatggtggc gttcgccttc tccagttcac tggccttgtt atcgcgctgc tctttgtagg
     1741 cgattgcgtt atcacggtaa tgattaacag ccaatgacag gcagacgatg atgcagataa
     1801 ccagagcgga gataatcgcg gttactctgc tcattgtttc ccccacaaac agacttcacg
     1861 ctcaatctca cggcgagtca tcagtccttt ccattgatta ccgccagcgt atgtccagcg
     1921 ccgtagctga tcacatgcgc ctttaatatc accctggttt attttgcgaa gaagcgtcga
     1981 tgttctgaaa ttgccagcac ccacgttgta gacgaacgag taaagagcgc agcgcgttgt
     2041 ttccggtata tcgactttga tgtacgggtt aatttgtctg gcgacagtgg caaggtcttt
     2101 attcaggagg gctttgcact ctgcttcggt atacgtttta ccgggaatga tgtcttttcc
     2161 tgtatgcccg taacatacag tccatacacc aactatgtct ttgtaaggat tatgtctcac
     2221 accttccaga ccatcgttac cacctgggcc agtgattaac acagatgcta tagcaatagc
     2281 cccgccacca atagcagcag caacagcttt tcgtaatgat ggaggcatta tccacctctc
     2341 gcagccttgc gcttatcttc tttaatcttg aaataaaggt ttgtcaggta cgtcagcagg
     2401 ccaaatacca ggctacccag cactccaatt gccgcccact gtgagggcgt gactttatct
     2461 agcagctgta aaaaccagta cccggcacta cctgctgagg tgccataggc gacacccgtt
     2521 gttaacttat ccatggattt cataacccca cctcgcagac aaagcgggtg taaattgagg
     2581 gaatactacg aaacgtaaca gactcggagt cagtgaataa ctcaggtatt gggttatcag
     2641 ctaatatcga gactcaaaaa atggaaaaac ccgctcgacg gcgggtttaa gctgtgtgac
     2701 gaagtaacca ctcttaacag cataac
//
LOCUS       DAEEKQ010000310         2721 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.316, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000310 DAEEKQ010000000
VERSION     DAEEKQ010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.316"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            30..2546
                     /locus_tag="KLK57_004282"
     CDS             30..2546
                     /locus_tag="KLK57_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE0894475.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQTSSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWNYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDKNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLNGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHTHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVIVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            2557..>2721
                     /locus_tag="KLK57_004283"
     CDS             2557..>2721
                     /locus_tag="KLK57_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0894476.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGRE"
BASE COUNT          778 a          657 c          630 g          656 t
ORIGIN      
        1 caaatataaa aaacacaggt catcagggaa tgccacaacg acaccaccag ggacataaac
       61 gcacaccgaa acagttggcg ctcatcatca aacgttgttt gccgatggtg ctcactggca
      121 gcggcatgct ttgcactacc gctaacgccg aagagtatta tttcgacccc attatgctgg
      181 aaactacaaa aagtggtatg caaacaaccg atctgtcacg tttttcaaaa aaatacgcac
      241 aactaccagg aacttatcag gttgatatct ggctgaataa aaagaaggtt tcacagaaaa
      301 aaattacatt taccgccaat gcagagcaac ttctgcagcc acagtttacg gtagaacaac
      361 tacgtgagct gggtattaag gtggatgaaa tcccggcgct ggctgaaaaa gatgacgata
      421 gcgtgatcaa ctcgcttgaa caaatcattc ccggtacagc agctgaattt gatttcaatc
      481 atcagcgact taatttgagc attccccaaa ttgcactgta ccgtgatgca agaggttacg
      541 tctccccttc tcgttgggac gatggtatac caacgctgtt taccaactac tcgtttacag
      601 gttctgataa ccgttaccgc cagggcaatc gtagccaacg acagtacctg aatatgcaaa
      661 atggtgctaa ttttggcccc tggcgattac gcaactattc cacatggaca cgcaacgatc
      721 agacatcaag ctggaatacc atcagtagtt atttacaacg tgatatcaag gcattgaagt
      781 ctcagttgct tctgggagaa agcgccacca gcggcagtat tttttccagc tacaccttta
      841 ctggcgttca actcgcttcc gacgataata tgttgccaaa cagccagcgc ggatttgccc
      901 caacggtacg cggtatcgca aacagtagtg caatcgtgac tatcaggcaa aatggttatg
      961 tgatctatca aagcaacgtg ccagcgggtg cctttgaaat taacgatctc tacccctctt
     1021 ccaacagcgg cgatttagaa gtcacgattg aagaaagtga cggtacgcaa cgtcgcttta
     1081 tccagcctta ttcttcatta cccatgatgc agcgacctgg gcatctaaaa tatagcgcga
     1141 ccgctggacg ctatcgcgct gatgcaaaca gtgatagcaa agaacccgaa tttgctgaag
     1201 ccacggcaat atatggtttg aataatactt ttacgctgta tggtggcctg ctcggttctg
     1261 aagattatta tgcgctgggg atcggtatcg gcggcacact tggcgcactg ggcgcgttgt
     1321 cgatggatat caacagagct gacacccaat tcgataacca gcactctttt catggctatc
     1381 aatggcgtac gcagtacatc aaagatatcc cggaaaccaa caccaatatc gctgtcagct
     1441 actatcgcta taccaacgat ggctatttta gttttgatga agccaatacc cgtaattgga
     1501 actataacag tcgccaaaaa agtgaaattc aattcaacat cagccagaca atatttgatg
     1561 gggtaagtct gtatgcctcc ggttcgcagc aagactattg gggcaataac gataaaaaca
     1621 ggaatatctc agttggggtt tccggtcagc aatggggaat tggttacagc ctgaattatc
     1681 aatacagtcg ctacacggat caaaataatg accgcgcact ctctttgaat ctcagtattc
     1741 cgttagaacg ctggttaccg cgtagccggg tttcctatca gatgaccagc cagaaagatc
     1801 gcccaaccca acatgaaatg cgtcttaatg gctcactgct ggatgatggt cgcctgagct
     1861 atagcctgga acaaagtctg gatgacgata acaaccataa cagtagcctg aacgccagtt
     1921 accgttcacc ttatggcacc ttcagtgccg gatacagcta cggtaatgac agcagccaat
     1981 acaattacgg cgttaccggc ggcgtggtta tccatactca tggcgtgacg ctctcgcaat
     2041 atctgggcaa cgcttttgcg cttatcgatg ctaatggggc ttctggcgtg aggatacaaa
     2101 actatccggg gattgctact gatccctttg gctatgcagt ggttccttat ctcacgactt
     2161 accaggaaaa ccgtctctcg gtagatacta cgcagctgcc cgataacgtc gatcttgagc
     2221 aaacaacaca gtttgtggtg cccaacagag gtgcaatggt agcggcgcgt ttcaacgcca
     2281 atatcggtta tcgcgtactt gttatagtca gcgatcgcaa cggtaaaccg ttgccctttg
     2341 gcgctcttgc cagcaacgat gagacggggc aacaaagtat cgtcgatgag ggcggcatac
     2401 tatatctctc tgggatatcg agtaaatcac aaagctggac tgtacgctgg ggaaatcagg
     2461 cagatcaaca atgtcagttt gcttttagta caccggattc agaaccaaca acctctgtat
     2521 tacaaggcac ggcgcagtgc cattaaggat aaaaaaatga aaagagcgcc tcttataaca
     2581 ggacttttgt tgatatccac atcctgcgct tatgcctcct caggagggtg tggagctgac
     2641 agcactagcg gtgcgacaaa ttacagcagt gtggttgatg atgttacggt gaaccagaca
     2701 gataacgtga caggacggga g
//
LOCUS       DAEEKQ010000311         2711 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.317, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000311 DAEEKQ010000000
VERSION     DAEEKQ010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2711
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.317"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            65..649
                     /locus_tag="KLK57_004284"
     CDS             65..649
                     /locus_tag="KLK57_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0894477.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            754..1494
                     /locus_tag="KLK57_004285"
     CDS             754..1494
                     /locus_tag="KLK57_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HBE0894478.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDNNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1529..2711
                     /gene="htrE"
                     /locus_tag="KLK57_004286"
                     /pseudo
     CDS             1529..2711
                     /gene="htrE"
                     /locus_tag="KLK57_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
BASE COUNT          772 a          572 c          587 g          780 t
ORIGIN      
        1 gtgtcatgaa tagtcattct tcaaaccagt agtctgacta atggaatttt attcaaggat
       61 ttttatgtct aaaaaattag gttttgccct gtctggtctt atgctggcaa tggttgcagg
      121 tactgcttct gctgatatgg atggtggtca gttaaatatc agtggtctgg ttgttgataa
      181 cacctgtgaa acccgcgttg acggcggtaa taaagatggt ctgatcctgc tgcaaactgc
      241 aactgttggt gaaatcgatg ctggcgtgct gaatgacacc gttggcgcta aagccaaacc
      301 tttcagcatc accgttgact gcagcaaagc aaatccgaat ccaggtagta cagctaaaat
      361 gaccttcggt tctgttttct ttggtaatag caaaggaacg ctgaacaacg acatgtctat
      421 taacaacccg tctgacggcg tgaatatcgc tctgcacaat attgatggtt ccactatcaa
      481 acaggttcaa attaacaacc ctggcgatgt gtatactaaa gccctggatg caacgacaaa
      541 atctgctgtt tatgatttta aagcgtctta cgttcgtgcc gtcgcagacc aaacagcaac
      601 tgctggttat gtaaaaacta acactgcata caccattact tatcagtaag tattcaccaa
      661 ttaaattgat tgaatgaata tacagggaat agtgttaata atttctattt ttatattatt
      721 ccctgtttta attaactctg tcagggatgg tttatgtttt ttaacaccaa acatacaaca
      781 gctttatgct tcgtaacctg tatggctttt agttcatcgt ctattgcgga cattgtcatt
      841 tcgggtactc gcgtaatata taaaagcgat caaaaaagtg tcaacgtacg tctggaaaat
      901 aaagggaata acccgttgct tgtccagagt tggttagata ctggcgataa caacgctgag
      961 cctggcagta ttacagtccc ttttactgcc acgccgccag tatcgcgtat tgatgccaaa
     1021 cgtgggcaaa caatcaaatt aatgtacaca gccagcacct cactgcctaa agacagagag
     1081 agcgtgttct ggtttaacgt actggaagtt ccaccaaaac cagatgcaga aaaggtcgcg
     1141 aatcaaagtc tgctgcaact ggcatttcgc acacgtataa aacttttcta tcgcccggat
     1201 ggattgaagg gaaatccctc tgaagccccg ttagccctga agtggttctg gtcaggttca
     1261 gaaggtaagg cgtcattgcg agtgaccaat ccaacccctt actacgtctc ttttagcagt
     1321 ggtgatttag aagctagcgg taaacgctat ccgattgatg tgaaaatgat tgcaccattt
     1381 agtgatgagg tcatgaaagt cactggcctt aatggcaaag caagttctgc aaaagtacat
     1441 ttttacgcca ttaatgattt tggtggcgcc attgaaggta atgccagtct gtaatcaggc
     1501 aaggatataa ttccgcagga agcataacgt gactataaaa ccaactaaaa attatcataa
     1561 tcatctgacc cgtatcgcta cgttttgcgc gctgctgtat tgcaatagtg ctttctgtgc
     1621 tgaacttgtt gaatatgacc ataccttcct gatggggcag aatgcctcaa atattgatct
     1681 cagccggtat agtgaaggta accctgctat accgggtatg tacgacgtca gtgtttatgt
     1741 caacgatcaa cctattatta accaaagcat tacatttatt gaaattgaag gaaaaaaaac
     1801 gcacaagcct gtatcacact taagaattta ttgcagtttc atataaattc tcccgatata
     1861 aataacgaaa aagccgttct gcttgccagg gatgaaacac tcggcaattg ccttaatttg
     1921 acggaaatta tccctcaggc ttctgttcgt tatgacgtta acgagcaacg tctggatata
     1981 gacgttcctc aggcatgggt aatgaaaaat taccaaaact atgttgatcc atcgttatgg
     2041 gaaaatggca ttaatgcggc catgttgtca tacaacctca acggatatca tagtgaaact
     2101 cctggtcgaa gaaatgacag catttatgct gcttttaacg gtgggatgaa tttaggtgca
     2161 tggcgactgc gtgcctcagg caactacaac tggatgaccg attctggcag taattatgat
     2221 tttaagaatc ggtatataca gcgtgatatc gcctcgctgc gttctcaact cattcttggt
     2281 gagtcttata caacgggcga aacctttgat tctgtcagta tccgcggcat tcgtttatac
     2341 agcgacagcc gcatgttgcc tccgacatta gcgagttttg cgcctatcat tcatggcgtt
     2401 gctaatacca acgccaaagt cactattacg caaggtggct ataagattta tgaaacgact
     2461 gtgccgccag gcgctttcgt cattgatgat ctgagtccgt caggttacgg cagcgatctt
     2521 attgttactg tcgaagaatc cgatggctca aagcggacat tctcgcaacc tttttcatcg
     2581 gtcgttcaaa tgttacgccc tggcgttgga cgttgggata ttagcggcgg ccaggtctta
     2641 aaagatgata ttcaggatga gcctaattta tttcaggcca gctactacta cggtctgaat
     2701 aactatctaa c
//
LOCUS       DAEEKQ010000312         2690 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.318, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000312 DAEEKQ010000000
VERSION     DAEEKQ010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2690
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.318"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            230..769
                     /locus_tag="KLK57_004287"
     CDS             230..769
                     /locus_tag="KLK57_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015870862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HBE0894479.1"
                     /translation="MSHGGWKEISHPQTLFTRQKGDGWTVVPKNLRIDFYTKDNDFTK
                     GLSVLSEVNKRHTEAQKGLTPSLNISKDDLKVLANARNIPQSKLEEEMMSQAIYRKEY
                     ATSNEKIKNYALYYHEQAQNIIQRHQDGLGNKNIDIAFITDKNHKKHLSDIFKVINES
                     GVKYDVIHFGACRVNREEK"
     gene            complement(995..1318)
                     /locus_tag="KLK57_004288"
     CDS             complement(995..1318)
                     /locus_tag="KLK57_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBE0894480.1"
                     /translation="MTKRSGSNTRRRAISRPVRLTAEEDQEIRKRAAECGKTVSGFLR
                     AAALGKNVNSLTDDRVLKEVMRLGALQKKLFIDGKRIGDREYAEVLIAITEYHRALLS
                     RLMAD"
     gene            1604..1798
                     /locus_tag="KLK57_004289"
     CDS             1604..1798
                     /locus_tag="KLK57_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HBE0894481.1"
                     /translation="MNKQQQTVLNMAGFIKSQSLTLLEKLDALDADEQAAMCEKLHEI
                     AEELQNSIQTRFEAESGTGV"
BASE COUNT          857 a          665 c          579 g          589 t
ORIGIN      
        1 aaaaaaaaca ataaaaaaca ccgcaaaaat ggattgttaa caaatgaaaa tactcaattc
       61 gaatctcata acaaacatat catcaacaat aagaaatcac cttctaacat catccaaaaa
      121 tataaaaaca cacccatcac caaacatgga cgtaacaagt catcgtcgtt tattccagaa
      181 agacggaata ggattgagcc aaacaggaaa agcaacacaa cttgtcatta tgtcgcatgg
      241 tggatggaaa gagatctccc atccacaaac actattcacg cgtcaaaaag gcgatggatg
      301 gactgtagtc cccaaaaacc taagaattga tttttataca aaagacaacg acttcacaaa
      361 gggactaagc gttctttcag aggtaaacaa acgccatact gaagctcaaa aagggttaac
      421 accatcacta aacataagca aagatgacct aaaagttcta gcgaacgcgc gcaatatacc
      481 tcaatcaaaa ttagaagaag aaatgatgtc acaagccata tatagaaaag aatacgcaac
      541 aagtaatgag aagataaaaa actatgctct atattaccat gaacaagccc aaaatattat
      601 acaaagacat caagatggct taggaaacaa aaatattgat atagcattta taactgataa
      661 aaatcataag aagcaccttt ctgacatatt taaagtaata aatgaatcag gagtaaaata
      721 tgatgtaatt cactttggcg cttgccgagt aaatagagag gagaaataat catcatttac
      781 cataatccct taattgtacg caccactgaa acgcgctgaa cgcgattctg acgcagacac
      841 cacaaaaata gatgttttac cccggaaaaa caggaaggat gcgtcaggat cgttttcagc
      901 tcgctgcata gctatgcatg aaagcgaatg gagatcactt tgggagctta ccgtgttcat
      961 accgtctgtt ttcaacagtt ttctccccgg gaagctaatc tgccataagc ctggataaca
     1021 gggcacggtg atactccgta atagcgatca gcacctccgc atactccctg tccccgatac
     1081 gcttgccgtc gataaagagt tttttctgta acgcccccag tctcataact tctttcagca
     1141 cccgatcatc agtcagtgag ttaacgttct taccgagagc tgccgcccgt aaaaaaccag
     1201 aaacggtctt gccgcattca gcagcccttt ttctgatttc ctggtcttct tctgccgtca
     1261 gacgaacagg gcgactgata gcccgcctgc gcgtattact tccacttcgt tttgtcataa
     1321 aacatggctc cgtatctgac acggggtgtc ggggcgaagc cctgaccagg gtaattgtaa
     1381 tagcgtgcat gtatgcgcgg tataacaatt acacatcctg tcctgtcagc aagttcgaat
     1441 taccgacaaa ggcacggcag tttttttggc agtgacagta ttgtcaggca gaaatacccg
     1501 tcaatgcgtc agacggcaga ataccggcgg taaaacagtc gaaaagtggt acgcgatata
     1561 atcacacaac gcagatgaca gacagaacgg agaaaacaga aagatgaaca agcagcagca
     1621 aaccgtactc aacatggcgg gattcataaa aagccagagc ctgacgctgc tcgaaaagct
     1681 ggacgcactc gatgccgacg aacaggccgc catgtgtgag aagctgcacg aaatcgcgga
     1741 agaactccag aacagcatac agacacgctt tgaagcggaa agtggcaccg gggtataaca
     1801 gtgtatcctg ctccagacag gatgcagaac ggtataccgg tgcgtaagga gaaaatgtca
     1861 cataaggcgc tcttccgctt cctcgctcac tgactcgcta cgctcggtcg ttcgactgcg
     1921 gcgagcggtg tcagctcact caaaggcggt aatgcggtta tccacagaat caggggataa
     1981 cgccggaaag aacatgtgag caaaaagccg aaggcaggga caaaggccgc gcaggtggcg
     2041 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
     2101 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
     2161 cgctctcctg ttccgaccct gccgtttacc ggatacctgt ccgcctttct cccttcggga
     2221 agcgtggcgc tttctcatag ctcacgctgt tggtatctcg gcccggtgta ggtcgttcgc
     2281 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
     2341 aactatcgtc ttgagtccaa cccggtaaga cacgaattat cgccactggc agcagccact
     2401 gataaccgaa ttagaggagt aagacttgaa gtcatgcgcc tgttaaggct aaactgaaag
     2461 aacaagtttt ggtgactgcg ctcctccaag ccagttacct cggctcaaag agttggtagc
     2521 tctgagaacc ttcgaaaaac cgccttgcag ggcggttttt tcgtttacag gcagcagatt
     2581 acgcgcagaa aaaaaggatc tcaagaagaa cctttaattt tttttcacac ttggtatcct
     2641 actttaaccc ataaaataaa cacttacacc gacacaccct taaacaaaag
//
LOCUS       DAEEKQ010000313         2674 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.319, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000313 DAEEKQ010000000
VERSION     DAEEKQ010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.319"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            151..1317
                     /gene="nhaA"
                     /locus_tag="KLK57_004290"
     CDS             151..1317
                     /gene="nhaA"
                     /locus_tag="KLK57_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HBE0894482.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1383..2282
                     /gene="nhaR"
                     /locus_tag="KLK57_004291"
     CDS             1383..2282
                     /gene="nhaR"
                     /locus_tag="KLK57_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HBE0894483.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKSFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2320..>2674)
                     /locus_tag="KLK57_004292"
     CDS             complement(2320..>2674)
                     /locus_tag="KLK57_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HBE0894484.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQVGFQPSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
BASE COUNT          623 a          596 c          682 g          773 t
ORIGIN      
        1 ccgtcaaaaa ctcatctcac cgctgatggc gcaaattctt taattgctcg taaaaaacga
       61 gttattccta cactataatc tgattttaac gatgatttgt gcggggtaaa atagtaaaaa
      121 cgatctattc acctgaaaga gaaataaaaa gtgaaacatc tgcatcgatt ctttagcagt
      181 gatgcctcgg gaggcattat tcttatcatt gccgctatcc tggcgatgat gatggccaac
      241 agcggcgcaa ccagtggatg gtatcacgac tttctggaga cgccggttca gctccgggtt
      301 ggttcactcg aaatcaacaa aaacatgctg ttatggataa atgacgcgct gatggcggta
      361 tttttcctgt tagtcggtct ggaagttaaa cgtgaactga tgcaaggatc gctagccagc
      421 ttacgccagg ccgcatttcc ggttatcgcc gctattggtg ggatgattgt tccggcatta
      481 ctctatctgg cctttaacta tgccgatccg attacccgcg aagggtgggc gatcccggcg
      541 gctactgaca ttgcctttgc acttggtgtg ctggcgctgt tgggaagtcg tgttccgtta
      601 gcactgaaga tctttttgat ggctctggct attatcgacg atcttggggc catcattatc
      661 atcgcattgt tctacactaa tgacttatcg atggcctctc ttggcgtcgc ggctgtagca
      721 attgcggtac tcgcggtatt gaatctgtgt ggtgtacgcc gcacgggcgt ctatattctt
      781 gttggcgtgg tgttgtggac tgcggtgttg aaatcggggg ttcacgcaac cctggcgggg
      841 gtaattgtcg gcttctttat tcctttgaaa gagaagcatg ggcgttctcc agcgaagcga
      901 ctggagcatg tattgcaccc gtgggtggcg tatctgattt taccgctgtt tgcatttgct
      961 aatgctggcg tttcactgca aggcgtcacg ctggatggct tgacctccat tctgccattg
     1021 gggatcatcg ctggcttgct gattggcaaa ccgctgggga ttagtctgtt ctgctggttg
     1081 gcgctgcgtt tgaaactggc gcatctgcct gagggaacga cttatcagca aattatggcg
     1141 gtggggatcc tgtgcggtat cggttttact atgtctatct ttattgccag cctggccttt
     1201 ggtagcgtag atccagaact gattaactgg gcgaaactcg gtatcctggt cggttctatc
     1261 tcttcggcgg taattggata cagctggtta cgcgttcgtt tgcgtccatc agtttgacag
     1321 gacggtttac cggggagcca taaacggctc ccttttcatt gttatcaggg agagaaatga
     1381 gcatgtctca tatcaattac aaccacttgt attacttctg gcatgtctat aaagaaggtt
     1441 ccgtggttgg cgcagcggag gcgctttatt taactccaca aaccattacc ggacagattc
     1501 gagcgctgga agagcgcctg caaggcaaat tatttaaacg caagggacgt ggtctcgaac
     1561 ccagcgagct gggagaactg gtctatcgct atgccgataa aatgttcacc ttaagccagg
     1621 aaatgctgga tattgtgaac tatcgcaaag aatccaattt attgtttgac gttggcgtgg
     1681 ctgatgcact ttccaaacgc ctggtcagta gcgtacttaa cgccgcagtg gtagaaggcg
     1741 agcccattca tcttcgctgc ttcgaatcca cccacgaaat gctgctggag caattaagtc
     1801 agcataaact ggatatgatc atttctgact gtccgataga ctctacgcag caggaaggcc
     1861 tgttctccgt gagaattggc gaatgtggcg tgagtttctg gtgtacaaat ccaccaccag
     1921 aaaaatcgtt cccggcttgt ctggaagaac ggcgactttt gattcctggg cgacgttcaa
     1981 tgttagggcg caaattgctt aactggttta actcccaggg attaaacgta gaaatcctcg
     2041 gcgagtttga tgatgccgcg ttgatgaaag cttttggtgc gatgcacaat gcaatcttcg
     2101 ttgccccaac gctttatgca tatgactttt atgccgataa aactgtcgta gaaattggtc
     2161 gcgtcgagaa tgtgatggaa gagtaccatg ctatttttgc tgagcggatg attcagcacc
     2221 cggcggtaca gcgaatctgc aatacggatt attctgcgct ttttagtcca gcggcgcgtt
     2281 aatcggcacc tcccccaaag ttaaggtggg ggagatagat tagttgtaca taaccacgat
     2341 tttgactcgg ctcattattt gcccgcttga gacattattt ccatatgtac gcgggcgaat
     2401 aaatagagga atctgattac ttccttcatg gggatgctga aaagagtagt aattgctttt
     2461 accatactgg atttcattcg taccatcatt ggagggttga aagcctactt gacccgcacc
     2521 gtcctcactg ttttctaact cattttttaa tatgctggtt cctgactcct gtatcgtcac
     2581 tggttcaaag taatattcaa catcactggg ttcaaaaact aattcacctt gctcgccctg
     2641 gatgcagtca aatcttatat gatgaaactg ataa
//
LOCUS       DAEEKQ010000314         2644 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.320, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000314 DAEEKQ010000000
VERSION     DAEEKQ010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.320"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            12..143
                     /locus_tag="KLK57_004293"
     CDS             12..143
                     /locus_tag="KLK57_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HBE0894485.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVERMGSWPEVVMA"
     gene            403..1398
                     /gene="pgl"
                     /locus_tag="KLK57_004294"
     CDS             403..1398
                     /gene="pgl"
                     /locus_tag="KLK57_004294"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBE0894486.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTCWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIVAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1439..2392)
                     /locus_tag="KLK57_004295"
     CDS             complement(1439..2392)
                     /locus_tag="KLK57_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HBE0894487.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
BASE COUNT          673 a          588 c          648 g          735 t
ORIGIN      
        1 ggggatttac catgcttatt ggctatgtac gcgtatcaac aaatgaccag aataccgatc
       61 tacaacgtaa tgcgtttatt tgtgccggaa gtgagttgat tgttgagcga atgggaagtt
      121 ggccagaagt tgttatggca tagtgtttac ggtcgttgat acaggtaaac aaaagcgggg
      181 agagtcatac tccctgaata gtcgcgaaaa tatctgtggc attgtcaaat tcgtgtcggt
      241 gactaatatt ttagtaatat gccattcgaa atggagtgct aacttattga tttaattgat
      301 gtgcgatatt gtattttatc gtgttttgtt gtctgttttt tatccagtaa gtgttgttat
      361 gtgtatgaaa gataagggat ttatcatttt tgaggtgtgt tcatgaagca aacagtttat
      421 atcgccagcc ctgagagcca gcaaattcac gtctggaacc tgaatcatga aggcgcactg
      481 acgctgacac aggttgtcga tgtgccgggg caggtgcagc cgatggtggt cagcccggac
      541 aaacgttatc tctatgttgg tgttcgccct gagtttcgcg tcctggcgta tcgtattgcc
      601 ccggacgatg gcgcactgac ctttgccgca gagtctgcgc tgccgggtag tccgacgcat
      661 atttccaccg atcaccaggg gcagtttgtc tttgtaggtt cttacaatgc gggtaacgtg
      721 agcgtaacgc gtctggaaga tggcctgcca gtgggcgtcg tcgatgtggt cgaggggctg
      781 gacggttgcc attccgccaa tatctcaccg gacaaccgta cgctgtgggt tccggcatta
      841 aagcaggatc gcatttgcct gtttacggtc agcgatgatg gtcatctggt ggcgcaggac
      901 cctgcggaag tgactaccgt tgaaggggcc ggcccgcgtc atatggtatt ccatccaaac
      961 gaacaatatg cgtattgcgt caatgagtta aacagctcag tggatgtctg ggaactgaaa
     1021 gatccacacg gcaatattga atgtgtccag acgctggata tgatgccgga aaacttctcc
     1081 gacacctgtt gggcggctga tattcatatc accccggatg gtcgtcattt atacgcctgc
     1141 gaccgtaccg ccagtctgat taccgttttc agcgtttcgg aagatggcag cgtgttgagt
     1201 aaagaaggct tccagccaac ggaaacccag ccgcgcggct tcaatgttga tcacagcggc
     1261 aagtatctga ttgtcgccgg gcaaaaatct caccacatct cggtatacga aattgttggc
     1321 gagcaggggc tactgcatga aaaaggccgc tatgcggtcg ggcagggacc aatgtgggtg
     1381 gtggttaacg cacactaacc gctgatttac ccggcgcagt ctctcctgcg ccggtgtatt
     1441 aacctatctc ctgtaacgca tgtctctggc gttcgacgat attggtccac aaattgtctt
     1501 tatcgtcagt ccacaaatta atcagcaagg caaaaaagcg ttctgctgcc ggagaaagca
     1561 cggcatcttt gcggcgaata atccccaatg tccgacgtat aacgggttcc accagtggaa
     1621 taccaataat cgaagaatag ggcgcatggg gcattgccag accaggaagc gccgaaatcc
     1681 ccagtcccgc ctctaccagt cctaatgacg tcgaaagatg acgcacttcg taaaaccagt
     1741 ccagcttcca gggcttgtcg gccagctgtt gttctatcaa cagtcggttg ccgctggagg
     1801 agcgtacgcc aatcattttg taacccacta actcctgcca ttctacaagc tgctttttgg
     1861 ccaatggatg gtcacgccga caggccagca cgaacggctc gttgaccagt ggggtaaaat
     1921 caatggatga atttgtgacg ttgttcatgt ttatgccaaa gtctgattcg ttacacagca
     1981 ccgattccat gcaattattc gtgccttgtt ccagaatccg cactttaata ttgggataca
     2041 gctcattaaa tttaccgatt gccagcggta aaaaataaaa tactgcggtc ggaatacacg
     2101 ctaacgtcac cataccacga tgataagcat tcatatcacg aatattaaaa agcgtttcat
     2161 cgaatttctt tattaattcc cgcgcctccg ggagcaacct ttttcctgct tttgttaacg
     2221 taaccttgcg agttgtacgc tcaaatagtt gaatatgtaa atcttcctcc atctttttta
     2281 ttcttcgcgt taatgcaggt tgcgtaatat tgagtaattt agcggcattg ttaaacgaac
     2341 tggactctgc cagtatgaca aatgccttca tacttgataa ttcatgcttc attattactc
     2401 cggaaaatgg aagcgacgat tttgggtggc tggccgttaa aaattttaac tgcatttagc
     2461 caacttaaat taatgaaaaa atgttattaa tcgttgagct aaagtcatta gagatgcttt
     2521 gcccttaatg taaccatatc gcaataagtt atgtttttaa attgagggca ttattatgaa
     2581 aaaaataccc tgggtaatga ctccaactta ttgatagtgt tttatgttca gataatgccc
     2641 gatg
//
LOCUS       DAEEKQ010000315         2636 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.321, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000315 DAEEKQ010000000
VERSION     DAEEKQ010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.321"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            111..443
                     /locus_tag="KLK57_004296"
     CDS             111..443
                     /locus_tag="KLK57_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HBE0894488.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            590..1951
                     /gene="yegQ"
                     /locus_tag="KLK57_004297"
     CDS             590..1951
                     /gene="yegQ"
                     /locus_tag="KLK57_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HBE0894489.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(2281..2598)
                     /locus_tag="KLK57_004298"
     CDS             complement(2281..2598)
                     /locus_tag="KLK57_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894490.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
BASE COUNT          692 a          688 c          621 g          635 t
ORIGIN      
        1 tgacattatc tctccgccct ggcaggcatc agaaaatcgc tcatacttta atcggtaaac
       61 agcaccttta gatgctgttt tcgatacgca atttcaatca aggagtcatt atggctggtt
      121 ggtttgaact cagtaagagc agtgataatc agttccggtt tgtgctaaaa gcgggcaatg
      181 gtgagactat cctcaccagc gagctgtata catcaaaagc ctctgcggaa aagggcatcg
      241 cgtcggtgcg tagcaacagc ccgcaagaag aacgctatga gaaaaaaacg gcaagtaacg
      301 gcaaattcta tttcaatctg aaagccgcca atcatcaaat tatcggctcc agccagatgt
      361 acgccaccgc gcaatctcgt gaaaccggaa ttgcctccgt taaagccaat ggcacaagcc
      421 agacggtgaa agacaatacg taattacgat gccgggcgtg gggtgtcggc gtccggcgct
      481 ttaccagatc cccccacagc gctacaatgc ccgcccttaa agtgggggca ctcccctaac
      541 cgcttcatca ggtgaagcgg agctgacctg tcatcagaac gagagaatta tgtttaaacc
      601 ggaactcctt tccccggcgg gaacgctgaa aaatatgcgt tacgctttcg cttatggcgc
      661 agatgctgtt tatgcgggcc agccgcgtta ctccctgcgt gtgcgcaaca acgaattcaa
      721 ccacgaaaat cttcagctcg gcatcaatga agcccacgcg ctggggaaaa agttttatgt
      781 cgtggtcaac attgcaccgc acaacgccaa gctgaaaacc tttatccgtg acctgaaacc
      841 ggtggtggaa atggggccgg atgcgctgat tatgtccgat ccagggctga ttatgctggt
      901 gcgtgagcac ttccctgaaa tgccgatcca cctctcggtg caggctaacg ccgtaaactg
      961 ggcgacggtg aaattctggc agcaaatggg cctgacccgc gtgatcctct ctcgcgagct
     1021 gtcgctggaa gagattgaag agatccgcaa tcaggtgccg gatatggaga ttgagatctt
     1081 cgttcacggt gcgctgtgca tggcctactc cggtcgctgc ctgctctctg gctatatcaa
     1141 caagcgcgat ccgaaccagg gtacctgcac caacgcctgc cgctgggagt acaacgtcca
     1201 ggaagggaaa gaagatgacg ttggcaacat cgtacacaag tacgagccga ttccggtgca
     1261 aaatgttgag ccgacgctag gtatcggcgc gccaaccgac aaagtgttta tgatcgaaga
     1321 agcccagcgt ccgggcgagt atatgaccgc gtttgaagat gagcacggca cttacatcat
     1381 gaactcgaaa gatctgcgcg ccatcgccca tgtagaacgc ctgaccaaaa tgggcgtgca
     1441 ttcgctgaaa atcgaaggcc gtactaaatc tttctactat tgcgcacgca ccgcacaggt
     1501 ttatcgtaaa gctatcgatg atgccgctgc gggcaaaccg ttcgatacca gcctgctgga
     1561 aactctggaa ggtctggcgc atcgtggcta taccgaaggt ttcctgcgtc gtcatactca
     1621 cgacgattat cagaactacg aatacggtta ttcagtttct gaccgccagc agtttgttgg
     1681 tgagtttacc ggtgagcgca agggggacct cgcagcggta gcggtgaaaa ataaattctc
     1741 cgttggcgac agccttgagc tgatgacgcc gcaaggcaac attaacttta cccttgagca
     1801 catggaaaac gccaaaggtg aagcgatgcc ggtcgcacca ggcgatggtt atactgtgtg
     1861 gctcccggtc ccgcaggatc ttgagctaaa ttacgcgctg ctgatgcgta atttctccgg
     1921 ggaaaccacg cgtaacccac acggtaagtg attaatttcg attatttttc ccggatggaa
     1981 aattcttaga aaccgatcac atacagctgc atttattaag gttatcatcc gtttcgctga
     2041 aaaacataac ccataaaatg ctagctgtac caggaaccac ctccttagcc tgtgtaatct
     2101 cccttacacg ggcttatttt ttatgcataa gccctatccc tggtcaccgt cttccattga
     2161 ccacatcgat agaatctccc ttcatagcac gatgcctttc acgtaacggc atcgtgctcg
     2221 cacaggttcc ggctaagcac aaccagaacg cgcatgtttg atgcttacca aaaaatattc
     2281 tcactctcca catttgaatg tcagacgagc gacgccatgt aatcctgcac cttctgtctt
     2341 caggtcaact atctgcattt ttttgccctg agtaacacag aaatgggctg catcattttt
     2401 tactatattt tctgcaccag atattcttcc cctagctaaa gaagcttcgg cttcggtgta
     2461 gtattggtta tcgagtttac gctgaatatt acttttatat gcaagaccaa atttaccgat
     2521 acttgtttca tcattatgca cagcacaacc agatataaga aaaatactaa ttaatgatat
     2581 agcagctatc tttttcatct caccttcccc cattaaatac caacgacact ctctag
//
LOCUS       DAEEKQ010000316         2619 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.322, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000316 DAEEKQ010000000
VERSION     DAEEKQ010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2619
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.322"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            40..159
                     /gene="hemP"
                     /locus_tag="KLK57_004299"
     CDS             40..159
                     /gene="hemP"
                     /locus_tag="KLK57_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000512926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HBE0894491.1"
                     /translation="MRRITSQTLLGPDGKLIIDHDGQEYLLRKTQAGKLLLTK"
     gene            complement(163..1599)
                     /locus_tag="KLK57_004300"
     CDS             complement(163..1599)
                     /locus_tag="KLK57_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HBE0894492.1"
                     /translation="MTLSFVTRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNGAGVWGGEALLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TAEPGAMLMRVAPSHLRFGHFEHFYYRRESEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FITEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSTASPLRDEFIDRAAFDGW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHGAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(1662..2375)
                     /gene="ydiV"
                     /locus_tag="KLK57_004301"
     CDS             complement(1662..2375)
                     /gene="ydiV"
                     /locus_tag="KLK57_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HBE0894493.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQVLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRGLLSLSQVYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTAEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
BASE COUNT          714 a          673 c          591 g          641 t
ORIGIN      
        1 gaaacggacg ccaatcacaa ggccgcttcc ccgcagccca tccggcgaat taccagccag
       61 acactgttag gtccggatgg caaactgatt atcgatcatg acgggcaaga atatctgctc
      121 cgtaaaactc aggctggcaa gctgctgtta actaagtagc ctttaactcg agcagctgac
      181 ttccagccgt ttaccccagt caggtggacg actgacatag tcatcatctc tatcgctgaa
      241 aggatttcgc aacgccccat gcaggcggtg caattccgtc atatcaccct tttctgccgc
      301 ttcaatcgcc cgttgcgcca accagttgcg caataccaga gcggggttaa cgctttgcat
      361 caactgctga cgctcactat cgctaacctc gtcttgttgc aaacgccccc gataacgggc
      421 aaaccagcca tcaaatgccg cacgatcaat aaactcatca cgtagcggtg acgccgtgct
      481 gtgctgctcg gtcagactca gcatgcggaa tgtgcgggta taatcgctgc gctctcgcgc
      541 catcagactg aataattcat tcagtagcgc gttatcctct ttttgctccg tgatgaagcc
      601 cagtttctgc cgcatccgtt gtccataatg cgtcaacaaa acctgctgat agctgtccag
      661 ggcctcattc agggcatcta cggcaacaaa tggtgacaat gtctgcgcca gacgctgtaa
      721 attccacaac gcgacagcag gttgattatc aaaactataa cgcccttgat gatccgagtg
      781 attacaaata aaaccgggtt cgtaatcatc aagaaaacca aacggcccgt aatcaagcgt
      841 cagtcccagc agcgacatgt tgtcggtatt catcacccca tgtgcaaagc cgaccgtctg
      901 ccattgggca attaacgacg cggtacgtgc gacaacatcg ctaaaccaga gacggtattt
      961 gtcctcatca tcttcaagat gtgaccagta atgacgaata gcaaagtcag ctaactgacg
     1021 aaccttttcc gactcgcggc ggtagtaaaa atgttcgaaa tgaccaaagc gcagatgact
     1081 tggtgccaca cgcatcagca tcgcgcctgg ctccgccgtt tcccgataca ctggcgaatc
     1141 gctggtgacg atacttaacg cgcgggtcgt cggaatgccc agataatgca tcgcctcact
     1201 ggcgagactt tctcgtatcg tcgaacgtaa aaccgcccgt ccatcaccca ttcgcgaata
     1261 aggcgtcagg ccagcacctt tcagatgcca gtccattgta gtgccatcag caagcagttg
     1321 ttcgccgagt aaaatgccgc gcccatcacc cagttggccc gcccagacgc cgaactgatg
     1381 accactgtaa acctgcgcca gtggtgacat accaggcagt agggcttcgc cgccccagac
     1441 acctgcgcca tttttaaaca gcgacgatgg aatactcagc gtgttagcca gttcggtatt
     1501 atgccaaatc agccgggcat tatttaaagg cgtaggggaa agtgctgtat aggtttctgg
     1561 caattcatcg cgccagcggg taacaaaaga cagggtcata gatcctcctg tttgatagtg
     1621 tagacggtta ctctcgtctt aaacaccagc aaacagaagg gttatcgctg aaccaacgtc
     1681 gttatctgat ttatgggaac ggcaggccac aggcatccct ggagagcatg gaaatcgaag
     1741 ggggtaatct gtgccaatat ttctgccgta tcaatgcctc ccgcaatgat gcaattgcaa
     1801 caaggggaaa tttgcgcctg gatcgcacga ataaacggtt cgaaggagcg gtgcgtgatt
     1861 tgttgctgaa taaaactttt atccagcatg acgcgggtga aaagaccatc aaaaaccgct
     1921 ttcattgtgc tattgcctgc tcccaaatta cctaacacaa ggggatacac ttgcgataag
     1981 gataaaagac cgcgattatc ttttccttca ttgagatgcg ggtaattctc attaatcaac
     2041 agttcaataa acggatattt taatagttcg cctgcgtaat tatccctctc taataataat
     2101 gtagccactt gtggcgttaa attaagccag gcaaagagtt tatgttgaat aaaaaaatgt
     2161 tggcatgatt ttagtaattc caactgctct gaaaataatt gccaatgttg ctcctcggta
     2221 agttgcgcta taacccgact cgtcgggata cgcaccgtgc catcttcact ggagaaatgg
     2281 gtaattaact caacgccgac aaggacttgc tggttatctc ttatcggcag gaaatagcaa
     2341 tcagaatgat aaagattctc caaaaaaatc ttcatggtaa ccgtccctct cgaaggatgt
     2401 ttttcagtat ccggctaaaa gggatgaggc cataagacat aacaattaca gaaggagtaa
     2461 ctttcatttg ttccatgtta accacttttt caggggtcct ttttttagat tatcctgatt
     2521 ataaacgaat aatccattta cggaattttt gtctgcaaaa tactactgta ttcaggagta
     2581 aatggacggc gatgtatcat cgccgtccat ttttcgaca
//
LOCUS       DAEEKQ010000317         2606 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.323, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000317 DAEEKQ010000000
VERSION     DAEEKQ010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.323"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(120..872)
                     /gene="yecC"
                     /locus_tag="KLK57_004302"
     CDS             complement(120..872)
                     /gene="yecC"
                     /locus_tag="KLK57_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HBE0894494.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(869..1537)
                     /gene="tcyL"
                     /locus_tag="KLK57_004303"
     CDS             complement(869..1537)
                     /gene="tcyL"
                     /locus_tag="KLK57_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HBE0894495.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1552..2538)
                     /gene="dcyD"
                     /locus_tag="KLK57_004304"
     CDS             complement(1552..2538)
                     /gene="dcyD"
                     /locus_tag="KLK57_004304"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HBE0894496.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGVIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCEALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEIILWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIGCISQKRFKDEGPILFIHTGGAPALFAYHPHV"
BASE COUNT          623 a          733 c          647 g          603 t
ORIGIN      
        1 gcaagccatc gcattctctt atgttattaa tgagttatgc tgatttgtta agcagtttta
       61 tcacgcttga aatggcgtcc cgccccgaca ggtgaatcgt cggggctgat tttttcttat
      121 tattgcagta gaaacttctc gaggaactgg cgggtgcgag gctgctgggg atcagcaaat
      181 aacgcttttg ctgccccctg ctcgactatc cgcccctggt ccataaaaat cgcccggtcc
      241 gcaacatccc gggcaaagct catttcgtgc gtcacaatca ccatcgtgcg cttttcctgc
      301 gccagctggc ggattgtgtt caggacttcg cccaccagct ctggatccag cgctgacgtt
      361 ggctcgtcaa acaaaatcac ctcaggacgc attgccagcg cacgcgcaat cgctacacgc
      421 tgctgttgac cgccagacaa acgacgtgga tagctggttt ctttaccggc cagcccaact
      481 ttcgccagca gctcgcgagc gcgcgccgtg gcctcttctt tcggttcgcc tttgacgatc
      541 accggccctt caataatgtt ttccagcacc gtacgatgcg gaaacaaatt aaagttctgg
      601 aagacaaacc cgacgtgctg acgcaactgg cggatcagcg atttttgctg acttaatgaa
      661 cgtgcagtat caatagtgat atcgccaacg gtgatcgttc ccgcttccgg ttgttccagc
      721 agatttatgc tgcgtagcaa cgtggttttg ccggaaccac tcggaccgat aattgccacc
      781 acttcgccag gctttacctc gaggtcgata ccgtgcagca ccgtctgacc gtgaaatttt
      841 ttcaccaggt tcttaacttc aatggcactc attttggttc tctctcctgg cgattaagtt
      901 gattctcaaa atggttctgc aacgtcgata atactgtcgc catgatccag tagatgagcg
      961 aagccgccag gtacatggtg aacacctcca gcgtgcgcga ggtaatcaac tgcgcctgac
     1021 ggaacagctc cggcacctgg attgtcgcgg ccagcgacgt atcctttacc aggctgataa
     1081 agctgttcga cagcggcggc aacgccaccc gcgccgcctg tggcaaaatc gcgcgacgca
     1141 tagtctgcca tggcgtcata ccaatactgg cagctgcttc ccactgacct ttatcaatag
     1201 aagaaatggc ggcgcgcagt gtttcggcgg cataggcggc agtgttcagc gacagaccaa
     1261 tcattgctga cggaatggga tctaattcga taccaaactg cggcaggccg taatagatca
     1321 taaacagttg tgcgatgagc ggtgtaccac gaaaaataga gatataaaaa cgcgccagcc
     1381 agcgcacggg ccagattggc gacaggcgca tcagcgcgag aataaacccg agcaataagc
     1441 caaaaaacat gccgccgata ctgagttgca gcgtataccc agcccctttc agcaggaacg
     1501 gcaaggaatc aataaccagt tgtatacttt cttgcatgag cgttcccgga gctaaacgtg
     1561 gggatgatag gcgaacagcg caggcgcacc gccggtatga ataaacagaa tcggcccttc
     1621 atctttgaag cgtttctgac tgatacagcc aatcagcccc gccatcgctt ttccggtata
     1681 cacaggatca agcagaatgc cttccagccg cgccagcaat ttcactgctt ccatgccttc
     1741 gtcgttcggc acgccgtagc caggtgcaaa atagtcatcc cagagtataa tttccgctga
     1801 tgcggtcagc tccagttctt tcgcaatcgc ctgttgtagg ttaaccactt tcggcaactg
     1861 atcggcaacg gaacgcgaca cggtcacgcc aatcagttcg ctttcaggca tcaggtgttc
     1921 cagcccaaca gccagtccgg cgtgagttcc ggcactgccc gatgcgacta ccaccgacga
     1981 aatattaacc gccccttcac actgttgcgc gatttccagc gcactctcca cataacctag
     2041 cgcgccaaga gcattagaac cgccaaccgg aatgacatac gggcgaaagc cttgtgcttc
     2101 gactcgcgtc gccagctctt ccagttgggc attgggatcg gtcagtgctt cgcacatttc
     2161 aatctgggta ttgaacagat ccagcaacaa gcgattgccg ttggttaaat agttttctgc
     2221 ggttgtgcca ataggatttt ccagcagcgc cacacagtgc agaccgagtt tcgccgcaac
     2281 tgcggcagtc tgccgcacat ggttagactg gatcaccccg gcagtaatca gcgtatcggc
     2341 accttcgcgc agagcatctg ccgcgagaaa ttctagctta cgtaatttat tgccgcccat
     2401 tgccatgggg gtgacgtcat cccgtttgat gaaaatttcc cgtcctagat aatcagaaaa
     2461 gcgcggcaga tattcgagcg gcgttggcgc gccgataaac tccaaccgtg gaaaacgggt
     2521 taaattatgc agtggcataa cagcctccga tgtatgttgt tgtgattttc ttattatgca
     2581 cgctgaaaac gcgtaaataa aaaagg
//
LOCUS       DAEEKQ010000318         2573 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.324, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000318 DAEEKQ010000000
VERSION     DAEEKQ010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2573
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.324"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..2412
                     /locus_tag="KLK57_004305"
                     /pseudo
     CDS             1..2412
                     /locus_tag="KLK57_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001475310.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            2414..2573
                     /locus_tag="KLK57_004306"
                     /pseudo
     CDS             2414..2573
                     /locus_tag="KLK57_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368382.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
BASE COUNT          699 a          534 c          729 g          611 t
ORIGIN      
        1 attttaggat atcaagcgaa cagtaatggt aaggttatcg ttgaaaaggg tggcgagtgg
       61 ctaataaaaa ataatgattc ctcaattgaa tttcaaattg gtaatcaagg agctggggag
      121 gcgactattc gcgagggtgg gctgattacg gctgaaaata cgattatcgg tggcaatgcc
      181 accggtatcg gaaccctgaa tgtgcaggat caagactctg tcatcacggt acgcagactc
      241 tataatgggt atttcggtaa tggcacagtc aatatttcca ataatggtct gattaataac
      301 aaagaatatt cattggtggg cgttcaggac ggttcccacg gtgtcgtcaa cgtgaccgat
      361 aaagggcatt ggagtttcct cggaacaggc gaagctttcc gctatatcta tatcggtgat
      421 gctggtgacg gtgaacttaa tgtctcgcgt gaaggtaaag tagattcggg aattatcact
      481 gcggggatga aagaaacagg cacaggcaac attactgtta aggataagaa ctccgttatc
      541 actaatctcg gaactaatct tggttatgac ggccacggcg aaatgaatat cagtaatgag
      601 gggcttgttg tcagcaacgg aggaagctca ctcggttatg gagaaaacgg cgtcgggaat
      661 gtcagcatca ccacgggggg gatgtgggag gtcaataaga atgtctatac caccattggt
      721 gttgcgggcg tcggaaacct caatattagc gatggcggta agtttgtatc gcaaaatatt
      781 acttttttgg gcgataaagc aagcggtatc ggcacactga acctgatgga tgcgacatca
      841 tcgttcgata ctgtgggtat caatgtcggt aattttggta gcggtatcgt aaatgtcagt
      901 aatggtgcca cccttaattc aacgggctat ggatttatcg gaggaaatgc ctccggtaag
      961 gggatagtta atatttcaac ggacagtctc tggaatttaa agacatcatc tactaacgca
     1021 caattgctac aggtcggtgt attaggcacg ggtgaactga atattaccac cggaggtata
     1081 gttaaagcgc gtgatacaca gatagctctc aatgacaaaa gtaagggcga cgtgagggtg
     1141 gatgggcaga actctcttct tgaaacattc aatatgtacg tagggacatc tggtacgggt
     1201 acgttaaccc tgacgaatag cggcacgctg aatgtcgaag gtggagaagt ttacttaggt
     1261 gtttttgaac ctgctgtagg aacgctaaac attggtgcag ctcacggtga ggcggcggca
     1321 gatgccgggt atataaccaa cgcgacgaaa gtagagtttg gttctggcga aggtgttttt
     1381 gtctttaatc atactaataa cagtgatgcc ggctaccagg tcgatatgct gattacaggt
     1441 gacgataaag acggaaaagt gatccatgat gcaggccata cggtgttcaa tgcagggaat
     1501 acttatagcg gtaaaacgct ggtcaatgac ggcctcctga ccattgcgtc tcatacggca
     1561 gatggggtaa cgggcatggg ttcgagtgaa gtcaccattg caagccccgg tacgctcgac
     1621 attctcgcat caacgaacag tgcaggagat tacacgctga ccaatgcgct caaaggcgat
     1681 ggcttgatgc gagtgcagct gtcatcctac gacaagatgt ttggctttac ccatgcaaca
     1741 gggactgaat tcgccggtgt tgcccaactg aaagacagaa ccttcactct ggaacgcgac
     1801 aacaccgctg cgcttactca cgcgatgttg cagtctgaca gtgaaaatac cacatcggta
     1861 aacgtgggag agcaatccat tggtggactg gccatgaatg gcggtacgct cattttcgat
     1921 acggatattc ctgctgcgac gcttgcagag ggatatatca gcgtcgatac gctggttgtc
     1981 ggcgcgggtg actacacctg gaaaggccgt aactatcagg taaacgggac gggcgacgtg
     2041 cttatcgacg tgcctaaacc gtggaatgat cccatagcga ataaccctct gacgacgctc
     2101 aatttgctgg aacacgatga tagccatgtc ggcgttcaac tggtgaaggc gcaaacggtt
     2161 attgggtcgg gtggctcatt aacgttacgt gatttacagg gcgacgaggt ggaagcggac
     2221 aaaacgttac acattgcgca aaacggaacg gtggtcgccg agggtgatta tggattccgc
     2281 ctcacgaccg caccaggtga tggtttgtac gttaactatg ggctgaaagc gctgaacatc
     2341 catggtgggc aaaagctgac gttagccgaa catggcggag cctatggcgc aacggccgat
     2401 atgtcggcaa aatagccatc gcttacacga ccgtctgggt gagccgcagt atacagattc
     2461 actgcattct cagggatcgg caagcagtat gtggatgcgt catgtcgggg ggcacgaacg
     2521 tttcaggacc ggtgacggcc agctaaatac tcaggctaac cgctatgtat tgc
//
LOCUS       DAEEKQ010000319         2563 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.325, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000319 DAEEKQ010000000
VERSION     DAEEKQ010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.325"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..1363
                     /gene="espL1"
                     /locus_tag="KLK57_004307"
                     /pseudo
     CDS             1..1363
                     /gene="espL1"
                     /locus_tag="KLK57_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1535..1642
                     /locus_tag="KLK57_004308"
     CDS             1535..1642
                     /locus_tag="KLK57_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894497.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1695..2453)
                     /locus_tag="KLK57_004309"
     CDS             complement(1695..2453)
                     /locus_tag="KLK57_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HBE0894498.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
BASE COUNT          767 a          575 c          535 g          686 t
ORIGIN      
        1 cttgattcat tgagaaatgg aaaaggcaaa gtcaacctga ttaaacacta ctcctccgtt
       61 gaatccatac agcagcatgt ccccttagtc cgggacgcgg agttcagagc attacttcgc
      121 catcctcctg cagggagtcg cgttatcgcg agtaaggatt ttggcttcgc tttagatatt
      181 ttcttttgtc gaatgatggc aaacaatgtc agtcatatgt ccgcgatttt atatatagac
      241 aatcatactt tgtcagtaag gctacgaata aagcagtcag tgtatggaca attaaattat
      301 gttgtgtctg tttacgaccc gaacgatacc aacgttgccg tcagaggcac ccacagggca
      361 gcacggggct ttctctcgct tgataagttc atcagttcag ctcccgatgc tcagacctgg
      421 gctgataggt atgttcgcaa ctgtgcaatt gctattcttc ctctattacc tgagggagtt
      481 ccagttgcta ttttagcggg tatcacgaca cgaatgccat ttgcccctat acatccatcg
      541 gcaatgttgt taataatggc cacaggccag actcaatagc ttattacact attcaaacag
      601 ttgcccatac tccctgaaaa agaaatcatt gaaataataa ctgcgcagaa tagcgttggt
      661 acacctgctt tatttctggc tatgatgaac ggacaaactg acaacgtaaa aatatttatg
      721 caagaaattc agtcactggt atacaatcat atcattcatg aagataatct ggttaaatta
      781 ctgcaaacta aaagtgctaa cgaaacacct ggactctata tctccatgtt gtatggattc
      841 gatgaaataa tcgatatctt tctgaatgca ttaaccactc caatagcaca agaactttta
      901 aacaaaaaaa atggtgatga gtattttagc catgaaaata catgatggtg agccaggatt
      961 atacgccgaa atggaaaata atcacccttt gtgtgtcaca cggttcctct ctaaaattaa
     1021 tggcatcgcc tttaaataca agttgagcaa agctaacatc atggatttat taaaaggagc
     1081 tacagcacag ggaacccctg cattatacat cgccatgagc aagggtaatg aagacgtcgt
     1141 gttatcttat atatcgacgc tgggtgcttt tgcaaaaaaa cattctttta gtcaacatca
     1201 gttatttaca ctattggccg ctaaaaatca tgacaacatg tcagctgttc atatagccat
     1261 tcatcataag cattataaaa ctgtagaaac atattatgct gctataaatg tcatcagcca
     1321 aagcctgagt tttagtgctg atgaaataaa gacgtattta taaccagcta tttacgatat
     1381 atatcgatac ataattttat ttttttcata aaacgcagaa acagtgacca tgtgcacaac
     1441 gccacccgac ggctgtaata acaaaaaatc ccttgatgcc tatccctttt gttacactcc
     1501 gttatcacgc acaagagata tgcaggacac tggtatgccg actaaacgct ttgataaaaa
     1561 acactggaag atggtggtgg tgctactggc aatctgtggc gctatgttgt tgctacgttg
     1621 ggcagcaatg atttggggct gagcagtgac aaacgaagac agccagaccg cataacacgg
     1681 tccggcagat aattttacat gctgtagccc agcgataagg ttgtccgacg atcggtatgt
     1741 tccggtgcag actctggagg ttcagagttc catgtgacgt tgtaagccac cttcagtcca
     1801 aaatgttcat taatagcaac atttaatgcg ctttcggagt tcagcgttgt atcttccgcg
     1861 ccaaagaccg aaacaccctg cgtaaattta gcgttgtcag tcaactgcca tgcataagcc
     1921 ccggaggcat agcccagcgg ctgggtttca ctggcattgt cggtatattt gtcgtaacgc
     1981 acacctgggc cgaattcaaa gcggaaactg tgtaccgggc cattcaaaaa ctggcgacca
     2041 taacccgcgg tcaacacatc gcgctctcga tagccgttat aacggtctgt cagccagctt
     2101 gcctgaccaa ataaatagtc ataatcagtt aaattaaaac ggctacgccc gcccgccgca
     2161 tatttttctg aagaacgctc atcattagaa gaagtattac tggcgttccc ccacagcgac
     2221 caggcagtgg tttgtccata ccaggtcatg gtggtgtcag ccgtaagtga ggagcttttc
     2281 gtattgcctg attgtgcaag atatcctgcg ttcagattac cttcgaaagg ttttttagcg
     2341 ctggcaggat cgtccatgac agtaaaaacg gaatcatcgg cagctgcatt cagtgacgca
     2401 aacatgcccc ccgccaacat aacgatggca ggaactgtct tcaaaagctt catttatcaa
     2461 gagtccgtac aacaaaaaaa gagaccatcg cggtcccgga aactttctta aggatcaaag
     2521 attagcgtcc ctggaaaggt aacgaattat aaaaaggcgc gaa
//
LOCUS       DAEEKQ010000320         2561 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.326, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000320 DAEEKQ010000000
VERSION     DAEEKQ010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2561)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2561)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2561
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.326"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..330
                     /locus_tag="KLK57_004310"
     CDS             <1..330
                     /locus_tag="KLK57_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0894499.1"
                     /translation="SEKVVIYYEKRGNAENYIKEAKYDMAVGHLLLKSFWANEAVFQM
                     MMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTARYVIMKLSENYPYKG
                     VYEKCLV"
     gene            580..1455
                     /locus_tag="KLK57_004311"
     CDS             580..1455
                     /locus_tag="KLK57_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-27"
                     /protein_id="HBE0894500.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGGYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESR
                     RDVLASAARIIAEGL"
     gene            1490..2188
                     /locus_tag="KLK57_004312"
                     /pseudo
     CDS             1490..2188
                     /locus_tag="KLK57_004312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(2253..>2561)
                     /locus_tag="KLK57_004313"
     CDS             complement(2253..>2561)
                     /locus_tag="KLK57_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HBE0894501.1"
                     /translation="RFINTDKAPAYGRALALLKREGRCPSDVEHRQIKYRNNVIECDH
                     GKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYYGDPLGEMRLVSRVFEM
                     "
BASE COUNT          661 a          613 c          685 g          602 t
ORIGIN      
        1 tctgaaaaag tagttatata ctatgaaaag cgtggtaatg ctgaaaacta tatcaaagaa
       61 gccaaatacg acatggcggt gggtcatctc ttgctaaagt cattttgggc gaatgaagcc
      121 gtgtttcaaa tgatgatgct ttcatataac ctatttttgt tgttcaagtt tgattccttg
      181 gactcttcag aatacagaca gcaaataaag acctttcgtt tgaagtatgt atttcttgca
      241 gcaaaaataa tcaaaaccgc aagatatgta atcatgaagt tgtcggaaaa ctatccgtac
      301 aagggagtgt atgaaaaatg tctggtataa taagaatatc atcaataaaa ttgagtgttg
      361 ctctgtggat aacttgcaga gtttattaag tatcattgca gcaaagatga aatcaatgat
      421 ttatcaaaaa tgattgaaag gtggttgtaa ataatgttac aatgtgtgag aagcagtcta
      481 aattcttcgt gaaatagtga tttttgaagc taataaaaaa cacacgtgga atttagggaa
      541 tactgatgta acacggattg accgtattgg gagtttgaga tggtgacaaa gagagtgcaa
      601 cggatgatgt tcgcggcggc ggcgtgcatt ccgctgctgc tgggcagcgc gccgctttat
      661 gcgcagacga gtgcggtgca gcaaaagctg gcggcgctgg agaaaagcag cggagggcgg
      721 ctgggcgtcg cgctcatcga taccgcagat aatacgcagg tgctttatcg cggtgatgaa
      781 cgctttccaa tgtgcagtac cagtaaagtt atggcggccg cggcggtgct taagcagagt
      841 gaaacgcaaa agcagctgct taatcagcct gtcgagatca agcctgccga tctggttaac
      901 tacaatccga ttgccgaaaa acacgtcaac ggcacaatga cgctggcaga actgagcgcg
      961 gccgcgttgc agtacagcga caataccgcc atgaacaaat tgattgccca gctcggtggc
     1021 ccgggaggcg tgacggcttt tgcccgcgcg atcggcgatg agacgtttcg tctggatcgc
     1081 actgaaccta cgctgaatac cgccattccc ggcgacccga gagacaccac cacgccgcgg
     1141 gcgatggcgc agacgttgcg tcagcttacg ctgggtcatg cgctgggcga aacccagcgg
     1201 gcgcagttgg tgacgtggct caaaggcaat acgaccggcg cagccagcat tcgggccggc
     1261 ttaccgacgt cgtggactgt gggtgataag accggcagcg gcggctacgg caccaccaat
     1321 gatattgcgg tgatctggcc gcagggtcgt gcgccgctgg ttctggtgac ctattttacc
     1381 cagccgcaac agaacgcaga gagccgccgc gatgtgctgg cttcagcggc gagaatcatc
     1441 gccgaagggc tgtaactggt tttgttgaat aaatcgaact tttgctgagt tgaaggatca
     1501 gatcacgtat cttcccgaca acgcagaccg ttccgtggca aagcaaaagt tcaaaatcac
     1561 caactggccc acctacaata aagccctcat caaccgtggc tccataactt tctggctgga
     1621 tgatgaagct attcaggcct ggtatgagtc agcaacacct tcttcacgag gcagacctca
     1681 gcgctattct gaccttgcca tcacgactgt gctggtcatt aaacgcgtat tcaggctgac
     1741 cctgcgcgct gcgcagggct ttattgattc cattttttct ctgatgaacg ttccgctacg
     1801 ctgcccggat tacagctgtg tcagcaggcg ggcaaagtcg gttaatatca gtttcaaaac
     1861 gcccacccgg ggtgaaatcg cacacctggt aattgattcc accgggctga aggtcttcgg
     1921 tgaaggcgag tggaaagtca aaaagcatgg ccaggaacgc cgtcgtatat ggcgtaagct
     1981 gcatctggca gttgacagta aaacacatga aatcatctgc gctgacctgt cgctgaacaa
     2041 cgttacggac tcagaggcct tccccgggtt aatccggcaa acccaccgga aaatcaggtc
     2101 agccgccgcc gatggagctt acgatacccg gctctgtcac gatgaactgc ggcgtaagaa
     2161 aatcagcgcg cttatcccgc cccgaaaagg tgcgggttac ggcactgttg caaagttagc
     2221 gatgaggcag ccttttgtct tattcaaagg ccttacattt caaaaactct gcttaccagg
     2281 cgcatttcgc ccaggggatc accataataa aatgctgagg cctggccttt gcgtagtgca
     2341 cgcatcacct caataccttt gatggtggcg taagccgtct tcatggattt aaatcccagc
     2401 gtggcgttga ttatccgttt cagtttgcca tgatcgcatt caatcacgtt gttccggtac
     2461 ttaatctgtc ggtgttcaac gtcagacggg caccggcctt cgcgtttgag cagagcaagc
     2521 gcgcgaccat aggcgggcgc tttatccgtg ttgatgaatc g
//
LOCUS       DAEEKQ010000321         2557 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.327, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000321 DAEEKQ010000000
VERSION     DAEEKQ010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.327"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(58..261)
                     /gene="tatE"
                     /locus_tag="KLK57_004314"
     CDS             complement(58..261)
                     /gene="tatE"
                     /locus_tag="KLK57_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HBE0894502.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(390..1178)
                     /locus_tag="KLK57_004315"
     CDS             complement(390..1178)
                     /locus_tag="KLK57_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HBE0894503.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            1271..1654
                     /gene="crcB"
                     /locus_tag="KLK57_004316"
     CDS             1271..1654
                     /gene="crcB"
                     /locus_tag="KLK57_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HBE0894504.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="KLK57_004317"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1708..1917)
                     /gene="cspE"
                     /locus_tag="KLK57_004317"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HBE0894505.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(2092..>2557)
                     /gene="pagP"
                     /locus_tag="KLK57_004318"
     CDS             complement(2092..>2557)
                     /gene="pagP"
                     /locus_tag="KLK57_004318"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HBE0894506.1"
                     /translation="TFRENIAQTWQQPEHYDLYIPAITWHARFAYDKEKTDRYNERPW
                     GGGFGQSRWDEKGNWHGLYAMAFKDSWNKWEPIAGYGWESTWRPLADENFHLGLGFTA
                     GVTARDNWNYIPLPVLLPLASVGYGPVTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT          652 a          659 c          580 g          666 t
ORIGIN      
        1 tttgaagcgg tttttttgta tcgaacaaga tattgaggga gcgtcctgct cgccacgtca
       61 ctctttatga gagagctttt cagcctgaag atcaacgtct gcgccttttt tcgccgcagc
      121 atcgtcatca ttcatcgcct tcttgaaccc tttaatggcc gctccaaggt ctccgcccag
      181 cgtacgtaac ttcttagtcc caaacagcag aacgaccagc gccgcaacta ccagcagttt
      241 ggtaatacta atctccccca tagatacctt cttgacatat aaccggcaac agaacgcgcc
      301 ttaaaaacca gagaatatta acggtcattc gacgcgcgca cacaatagca atctgtaaca
      361 aggtgaatca agcgccgagt aaaaaaacat cataataatt gcggcggcgc aaagcgacgg
      421 ttgtttaaga cgggcagttg cgcgcgcacc tgacgcacac gttcgggcgt cacttccgcc
      481 ataatgagtg caggcatttc tgacgctgcc gcaatggtga cgccaaaggg atctataatc
      541 cggctttgcc cgatattttt gttcccgcac tcccccgccg ccaccatata acaggtggta
      601 tccagcgcac gagcggcaag caacgttgac cagtgatgct ctttgagcgg cccgcgaacc
      661 caggcggcag gaagtaccag gatttcagct ccctgtaatg cctgtgccag cgccagctct
      721 ggaaagcgta agtcataaca ggtcatcaga ccgaccttca tcccctccac ctcgagtaac
      781 ggagcgattt cattaccagc atcaacacgg cgtgattcct gaatggcaaa tgcatcatag
      841 agatgcaatt tggcataacg ggcgacgatg tttcctgcct gtagcgccac cagcatattc
      901 catgcgcgcc ccggcgttga aggaacatga atcgtcagaa ttgtcgtcat catgttacgt
      961 ttactttctc gccgtaaacg tccgaggaat tcgccttcca gcagctgtgc tgatttaacc
     1021 gatagatctg catcatgatc atcgcgcgcc agcaatgctt ccggcaggac gaacagcgat
     1081 acgtcgtttt ccgccgcctg cgccatcaac gaggcacaaa tctcagcgtt cttttcccac
     1141 acagatgtaa cagcaaactg tcctgcggca accagcattg ccacctcctg tcagagtcgt
     1201 aaaattcgcc aacgacgatt attcggcgtt acacttatca ctcatacaaa tcaaatagca
     1261 ggattttgca gtgttacaac ttcttttagc agtttttatt ggcggtggta cgggaagcgt
     1321 ggcgagatgg ctgttaagta tgcgatttaa cccgctgcat caggcgattc cgttggggac
     1381 gctggcagca aacctgattg gggcattcat cataggaatg ggattcgcct ggttcagcag
     1441 gatgacgaac attgatccag tgtggaaagt attaatcacc accggatttt gtggcggtct
     1501 aacaaccttc tcaacatttt cggcagaagt ggtgtttttg ttacaagagg gccgctttgg
     1561 ctgggcgtta ctgaacgttt tcgtcaacct tctggggtct tttgccatga ccgcactggc
     1621 tttctggctg ttttcggcct caaccgcaca ctaaagacca aaaaaaaccc gctgattaag
     1681 cgggttttga attcttgctg acgtatctta cagagcgatt acgtttgcag cagaagggcc
     1741 tttggcaccg ttagtgattt cgaactctac gcgctgacct tcagcaagag ttttaaaacc
     1801 attagtctgt attgcagaga agtgtacgaa cacgtctttg ctgccgtctt ccggagtaat
     1861 gaaaccgaat cctttggact cattaaacca cttaacgtta cctttaatct tagacatcaa
     1921 aattaccttt acatgaaaaa tagacacaaa tgctgtgtcg gttaccagta caccaattgt
     1981 ggtacgcttt gtccagtgca actttactaa aaagtgataa aagtcgcgtt atttttgatg
     2041 ttacttattt tagttattga ttttaaagaa gttactaaaa cttcatttgt ctcaaaactg
     2101 aaagcgcatc caggcaaagt acacattgcc attgttgtag gtacccggaa tgtaggtcat
     2161 ctgaaaagtc actgggccat aacccacgga ggccaatggc agtagaaccg ggagagggat
     2221 gtaattccag ttatcgcgtg ccgttacgcc agcggtgaat cccagaccta aatgaaaatt
     2281 ttcatccgcc agcggtcgcc aggtactttc ccatccgtat ccggcaatcg gttcccattt
     2341 gttccacgag tccttaaatg ccatggcata caggccatgc cagtttcctt tttcatccca
     2401 tcgcgactga ccaaaaccgc caccccacgg tcgctcgtta tagcgatcgg ttttttcttt
     2461 gtcgtaagcg aaacgtgcat gccaggtgat ggcaggaata tataaatcat aatgttcagg
     2521 ctgttgccag gtttgtgcaa tattttctct aaacgtt
//
LOCUS       DAEEKQ010000322         2539 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.328, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000322 DAEEKQ010000000
VERSION     DAEEKQ010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.328"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(107..1525)
                     /gene="phrB"
                     /locus_tag="KLK57_004319"
     CDS             complement(107..1525)
                     /gene="phrB"
                     /locus_tag="KLK57_004319"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HBE0894507.1"
                     /translation="MTTHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPSPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGVGSVWLNEL
                     IWREFYRHLITYHPSLCKHRPIIAWTDRVQWQSNPAHLQAWQEGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPTTQGEKFDLEGEFIRQWLPELRNVPGKSVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(1522..2031)
                     /locus_tag="KLK57_004320"
     CDS             complement(1522..2031)
                     /locus_tag="KLK57_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HBE0894508.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDYYNQYRQRVVVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYHDAWLSGQRYFELWPRV
                     INLRHSGVL"
BASE COUNT          642 a          659 c          579 g          659 t
ORIGIN      
        1 caggcctacg tatttcctgc aatttattga atttgcacaa atttgtaggc tggataaggc
       61 gttcacgccg catccggcat ctgggctcga ttgcctgacg cgtctgttat ttccccttcc
      121 gcgccgcctc atacgctgcc aacgtttgta ctctcgcctc tttgtgctcg actatcggtt
      181 gcggataatc cagcttcaca cctgctttct gcgcccactt ccacggctca tgcaccgatt
      241 tccccggtac attgcgtagt tccggtagcc actggcggat aaactcacct tcgagatcaa
      301 atttctcgcc ctgggttgtc gggttgaaaa tacgaaaata cggcgctgca tcggttccgg
      361 ttgaagcggc ccactgccag ccaccgttat tggctgccaa atcaccatca atcagctgcg
      421 acatgaaata tcgctcgcct tcgcgccagt cgatcaataa atctttcacc agaaaactgg
      481 ctgtaatcat ccgtagcctg ttatgcatcc agccagtgct gttaagctga cgcatagcgg
      541 catcaacaat cgggtatccc gttttgcctt cctgccaggc ctgtaaatgt gcgggattgc
      601 tctgccactg tacacgatcc gtccaggcaa taattggacg atgtttacac aacgaggggt
      661 gatacgttat caggtgacgg taaaactcgc gccagatcag ctcattaagc cagacactac
      721 cgaccccacc gtccagcgcc tgcggctgtt cagccaacaa gcgatgcaag cactggcgag
      781 gcgataaccc gcccgttgcc aggctggccg acaaacggct ggtgccttcc actgccggaa
      841 aatctcgttg ttgctcatat tctccggcac cgttctggca aaactggcgt aattgcgcaa
      901 tcgccgcttt ttcttccacc ggaaaatgcg cagtatcgaa agactgacga ggataattca
      961 gcgtaatgga tggcgagggc tctatcgatc cgctactacg aacttttggc gcagcgacgc
     1021 actccggcat cccttcccgc agccgtttca gccaggcatt cttaaaaggc gtaaagactt
     1081 tgtacatctc gtgattaccg gtcatcaccg cgccaggcgg caggatcacg ctgtcatcaa
     1141 atccttcaca caccacgtta cgcagcgctc tttcaacttc cacatcccgc gcccgctcat
     1201 tcacttcata ctgatagtta taaaacaggt gggtaacgct gttttccgcg cacacctgtt
     1261 taactatttc gacactggcg acaaagtcat ccacttcacg gaacaataaa ggaatacctt
     1321 tttccgcaag cgctatttgt agcccattca gttgagcatt gataagttca gcctgacgcg
     1381 gcgacatgtt atgcgtcgcc cactggcgtg gtgtagcgat atacaacgcc agcacgcgtg
     1441 cagacgaatt gcggcaggcg gcagccagtg cgagattatc gtgcagacgt aaatcttggc
     1501 gaaaccagac cagatgggta gtcataaaac tcctgaatgg cgcaagttaa tcacacgcgg
     1561 ccaaagttcg aaataacgct gccctgaaag ccaggcgtca tgataaagcg ccatatagtg
     1621 tttgatacgc atcagcggcg caagaagtgg ttgctcgcca cggcgataac tgtcgataag
     1681 cgcagccagc tgctggcgtt ctgtggaatc aatatgcggg cgaaaataac cctgaacgtg
     1741 catcaaaaca ttggtgtgat cgcgcgggtt ggcggggtga gaaagcaaaa caactacgcg
     1801 ttggcggtac tggttgtaat agtcatccag attttgccac tggtgaatat cagccacgaa
     1861 cgggccaagt ttgcggtact ccggctgaga atgcgccaga aagacgagtt tatagcggct
     1921 gtgataatcg agaagcgccc cgcgcgttaa gccatgttct ttcagtcgat gtaactcatg
     1981 aagggcaaag atacggatta gggtgctgtc atcaaatcgt tgtagattca ttactgctcc
     2041 tgaacggtat aaaggagtaa gtgtagacga tggtaaatga attttatggg gcagagtttt
     2101 tgcggggaag tattttgttg gtaaaaatat tatcaacttc atctgagtga gaattattct
     2161 actatttccc gagctgcatt cattttttgt attttttatt tttagaaaat aacagttctc
     2221 aaagcttaat tacctcacta ttaattgtat ttcaaactaa caatcgcact ccattttctc
     2281 tttttaaaaa acataaacac tgttaaaaca aatacttatt tcgttatctt aaatgctatt
     2341 tctacatcac tcgtccttat aatcaaatta ttcttattca tagctatttt tgcaggttta
     2401 aaatagcggc tttcaccaaa gtcagccatg aaaattagac tacaagtcag ggcaagatta
     2461 cgaaagcccg ctccccgcaa ggactgacgc cagatagttt ctgtccatgg ctgcttttcg
     2521 catcttacgt cttaaccct
//
LOCUS       DAEEKQ010000323         2461 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.329, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000323 DAEEKQ010000000
VERSION     DAEEKQ010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2461
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.329"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..325
                     /gene="casB"
                     /locus_tag="KLK57_004321"
     CDS             <1..325
                     /gene="casB"
                     /locus_tag="KLK57_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cse2/CasB"
                     /protein_id="HBE0894509.1"
                     /translation="VSANVKAIDERQPFAAQLAAVMSKGRFTRLSAVKTPDELLRQLR
                     RAVRLLNGSVNLDSLADGIFRWCQESDDLLNHHRRQQRPTEFIRIRWALEYYQAGDAD
                     NEQNQ"
     gene            337..1392
                     /gene="cas7e"
                     /locus_tag="KLK57_004322"
     CDS             337..1392
                     /gene="cas7e"
                     /locus_tag="KLK57_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HBE0894510.1"
                     /translation="MTTFIQLHVLTAYPAANLNRDDTGAPKTVVLGGATRLRVSSQSL
                     KRAWRTSALFEQALAGHIGIRSGRIAREAATILIEKGIEDKKAIEWAAKIADYLGKAK
                     NDKKPKDPLTNAETEQLVHISPAEFDAVKALAHQLAEEKRAPKEEDLALLRKDRIAVD
                     IAMFGRMLANKPEFNVEAACQVAHAFGVSETIVEDDFFTAVDDLRQASEDAGAGHLGE
                     TGFGSALFYTYICIDKDLLVENLGGDEALANQTIRAFTEAALKVSPTGKQNSFASRAY
                     ASWALAEKGTDQPRSLAAAFYEPINGTRQLDVAVQRITTLRENMNTVYEQKTECASFD
                     VMNKQGSMKDVLDFICA"
     gene            1403..2149
                     /gene="cas5e"
                     /locus_tag="KLK57_004323"
     CDS             1403..2149
                     /gene="cas5e"
                     /locus_tag="KLK57_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HBE0894511.1"
                     /translation="MSQYLIFQLHGPMASWGVDAPGEVRHTHELPSRSALLGLLAAGV
                     GIRRDDTERLNAFNRHYSLVVCASRNPRWARDYHTVQMPKEVRKARYFSRREELSDPE
                     LLSAIISRRDYYTDAWWMVAVATTADAPYSLEQLQDGLRHPVFPLYLGRKSHPLALPL
                     APLLLEGNACDALCNAYQQYQDHFHKLKVSLPKLQDECWWEGKHDGLVASKILRRRDV
                     PLNRQQWLFGERTVNQGPWLSKEEPCTSQE"
     gene            2131..2325
                     /gene="cas6e"
                     /locus_tag="KLK57_004324"
     CDS             2131..2325
                     /gene="cas6e"
                     /locus_tag="KLK57_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HBE0894512.1"
                     /translation="MYLSRITLHTGQLSPAQLLHLVDRGEYVMHQWLWDLFPGGKERQ
                     FLYRREELQGGAPIKLSSML"
     gene            complement(2320..>2461)
                     /locus_tag="KLK57_004325"
     CDS             complement(2320..>2461)
                     /locus_tag="KLK57_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HBE0894513.1"
                     /translation="LSRAYYTRKMSQGKRHNQALIALARRRCDVLFAMMRDGTFYTPQ
                     GS"
BASE COUNT          604 a          615 c          666 g          576 t
ORIGIN      
        1 agttagcgcc aatgttaaag ccattgatga acggcagcct tttgccgcgc aactggcggc
       61 ggtaatgtca aaggggcgtt ttactcgcct gtcagcagta aaaacaccgg atgaattact
      121 gcgccagtta cgccgcgccg tcagattatt aaacggttcc gtcaatcttg actccctggc
      181 agatggcatt ttccgctggt gccaggagag cgacgacctg ctcaatcatc accgccgcca
      241 gcagcggcca accgaattta tccgtatccg ctgggcgctg gaatattacc aggctggcga
      301 cgccgataac gaacaaaatc aataaaggac tatctcatga caacatttat tcagcttcat
      361 gtgttaaccg cttaccctgc tgccaacctt aaccgtgatg acaccggtgc gccaaaaact
      421 gtggtgctgg gtggagcaac gcgtctgcgc gtttcctcgc aaagtctgaa acgtgcgtgg
      481 cgcacttctg cactttttga acaggcactg gcgggccata ttggtattcg cagtgggcgt
      541 attgcgcgtg aggcggcaac tatcctgatt gagaaaggga ttgaagataa aaaagccatc
      601 gaatgggcgg caaaaattgc tgattatctt gggaaagcta aaaatgacaa aaaaccaaaa
      661 gatccgctca ctaacgccga aactgaacaa ttagttcata tcagcccggc agaatttgac
      721 gccgtaaaag cgcttgctca tcaactggca gaagaaaagc gcgcgccaaa agaggaagat
      781 ctcgcgttgt tacgtaaaga ccgtatagcg gtagatatcg ctatgtttgg tcgtatgctg
      841 gcgaataaac ctgagtttaa tgttgaagcc gcctgccagg tcgcgcatgc gtttggtgtc
      901 agtgaaacga ttgtcgaaga tgattttttc accgccgttg atgatttgcg ccaggcctct
      961 gaagatgccg gtgccgggca tctcggagaa accggattcg gttcagcact tttctacacc
     1021 tatatctgca ttgataaaga tctgctggta gaaaacctcg gcggtgacga agcgttagct
     1081 aatcagacca tacgcgcttt tacggaagcc gcgcttaaag tttccccaac cggtaaacaa
     1141 aatagctttg ccagccgtgc ctatgcttcc tgggcgctgg cagaaaaagg caccgatcaa
     1201 ccacgctctc tggcggcggc tttctatgaa cccattaatg gcacacgtca gttagatgtg
     1261 gcggtgcagc gtattacaac gcttcgcgaa aacatgaata cggtctacga acagaagacc
     1321 gaatgcgcaa gttttgacgt gatgaacaaa cagggaagca tgaaggacgt gctggacttt
     1381 atctgcgcgt aaggggcggc ttatgagcca atatctgatt tttcagcttc atgggccgat
     1441 ggcgtcctgg ggtgtcgatg ctccaggtga agttcgtcat acccatgaac ttccttcgcg
     1501 ctcagcattg ctggggctac tggctgccgg ggtagggatt cggcgtgatg ataccgaacg
     1561 cttaaacgcg tttaatcgcc actattcact ggtggtttgc gccagccgta acccgcgctg
     1621 ggcacgggat tatcacacgg tccagatgcc aaaagaggtg cgtaaagcac gttatttcag
     1681 ccgtcgtgaa gagttgagcg accctgagct tttgagcgcg attatctccc ggcgcgacta
     1741 ctacaccgat gcctggtgga tggtggcggt ggcaacaaca gccgatgcgc cttacagcct
     1801 cgaacagttg caggacggtt tacgccatcc agtttttccg ctttatctgg ggcgaaaaag
     1861 tcatccgctg gcattacctc ttgcgccgtt actgctcgaa ggcaacgcgt gtgatgcctt
     1921 atgtaatgca taccaacagt atcaggatca tttccataaa ctgaaagtct cactcccgaa
     1981 acttcaggat gaatgctggt gggaagggaa acacgacggc ctggtcgcga gcaaaatatt
     2041 acgccgccgg gatgttcctt taaatcgtca gcagtggctg tttggggaac gcaccgtcaa
     2101 tcaggggccg tggctcagca aggaggaacc atgtacctct caagaataac cttacatacc
     2161 ggccagcttt cgcctgcgca gttgctgcat ctggtggatc gcggtgaata tgtgatgcat
     2221 cagtggctgt gggatctttt ccctggcggc aaagaaaggc aatttcttta tcgtcgggaa
     2281 gaactccagg ggggtgcccc tattaagttg tcaagcatgt tatgacccct gcggggtata
     2341 aaaagtcccg tcgcgcatca tggcgaacag aacgtcgcag cgtcgtctcg ccagggcgat
     2401 aagcgcctga ttgtgtcgtt ttccctgact cattttgcgg gtgtagtaag ccctggagag
     2461 c
//
LOCUS       DAEEKQ010000324         2446 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.330, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000324 DAEEKQ010000000
VERSION     DAEEKQ010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2446
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.330"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(25..315)
                     /locus_tag="KLK57_004326"
     CDS             complement(25..315)
                     /locus_tag="KLK57_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HBE0894514.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(305..742)
                     /gene="ratA"
                     /locus_tag="KLK57_004327"
     CDS             complement(305..742)
                     /gene="ratA"
                     /locus_tag="KLK57_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HBE0894515.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            913..1395
                     /gene="smpB"
                     /locus_tag="KLK57_004328"
     CDS             913..1395
                     /gene="smpB"
                     /locus_tag="KLK57_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HBE0894516.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            1610..1972
                     /gene="ssrA"
                     /locus_tag="KLK57_004329"
     tmRNA           1610..1972
                     /gene="ssrA"
                     /locus_tag="KLK57_004329"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2171..>2446)
                     /locus_tag="KLK57_004330"
     CDS             complement(2171..>2446)
                     /locus_tag="KLK57_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4376 domain-containing protein"
                     /protein_id="HBE0894517.1"
                     /translation="GRIWNAGPDSLGRLSPVVMLAKSVTAQTHMAWGDADNQQVKLSM
                     PELEELAAAMVQAQVDRNDEIYRRQREMKEELSGLEDLGTIRAFDVT"
BASE COUNT          667 a          655 c          549 g          575 t
ORIGIN      
        1 caatgagcac cttttttctg tctgttattt attcgctgat ttttctgctc gttgcctgcg
       61 aagctctttc ggatcggcaa tgagagggcg ataaatctcc acccgatcgc catcatgcac
      121 actatcgctt agttttgccg gacggctgta aatgccgact ttgtttttag ttaaatcgat
      181 atcggtacgc aattccagca agccactggc gcgaatagct tcttcaaccg tcgcgccctc
      241 ctgcagcgtc acgcgctgca ggtactgctt ctcaggtagc gcataagcca cctcaacggc
      301 aattttacct agcactgtaa acctctttcg cacgaaccgt aaaagcctgg accatattag
      361 acgccagctc tttaaacacg cgaccaaagg cgagttcaat caacttattg gtaaactcaa
      421 agtcgagatg aaactcgata cgacacgcct cctggctcag cggcgtaaac ttccatccac
      481 caatcaattt cttgaacggc ccatccacca gattcataag aatactttgg ttactggtca
      541 actggttgcg ggtagtaaac gttttgctga tcccagcctt agagacatct accgccgcag
      601 tcatctgccc aggagtggac tccagaatcc gacttccggt acaacccggc aaaaactgag
      661 gataagactg aacgtcattc actaactgat acatttgctc cgcgctgtag ggtaccagtg
      721 cggtccggct aatctgaggc ataactattt ccatcaacaa aaatccaaca aataatatca
      781 tttatcctgc tgtaaaaaaa acgctatccc ggcgctgggt aacatcgggt tcatgctaag
      841 atagagcctt gtcccccgca ggattgatat ggggtgtttt cgatttcaga ttaccgatga
      901 ttcacgacgc ttatgacgaa gaaaaaagca cataaacctg gttcagcgac catcgcgctt
      961 aacaagcgcg cccgtcacga atactttatc gaagaagagt tcgaagcggg acttgccctg
     1021 caaggctggg aagttaaatc cctgcgcgca ggaaaagcca atatcagcga cagctacgtc
     1081 cttctgcgtg acggagaggc atttctgttt ggcgctaaca tcacgccaat ggccgtggcc
     1141 tccacgcatg tggtgtgcga tcctacccgt acccgcaagt tacttctcaa ccagcgcgaa
     1201 ctggactcat tgtacggtcg cgtcaatcga gaaggctata ccgtagtggc gctctccctg
     1261 tactggaaaa atgcctggtg caaagtgaaa atcggcgtcg ccaaaggtaa aaaacagcac
     1321 gataaacgtt cagatatcaa agaacgcgaa tggcaggtgg ataaagcacg tatcatgaaa
     1381 aacgcccacc gttaaacctg cactccaatt attgaccagt tcctcaccgc gcctccctct
     1441 ccggcggcgc gaatgaacat cttattggct atcacatccg acacaaatgt tgccatccca
     1501 ttgcttaatc gaataaaaat caggctacat gggtgctaaa tctttaacga taacgccatt
     1561 gaggctggtc atggcgctca taaatctggt atacttacct ttacacattg gggctgattc
     1621 tggattcgac gggatttgcg aaacccaagg tgcatgccga ggggcggttg gcctcgtaaa
     1681 aagccgcaaa aaatagtcgc aaacgacgaa aactacgctt tagcagctta ataacctgct
     1741 tagagccctc tctccctagc ctccgctctt aggacgggga tcaagagagg tcaaacccaa
     1801 aagagatcgc gtggaagccc tgcctggggt tgaagcgtta aaacttaatc aggctagttt
     1861 gttagtggcg tgtccgtccg cagctggcaa gcgaatgtaa agactgacta agcatgtagt
     1921 accgaggatg taggaatttc ggacgcgggt tcaactcccg ccagctccac cacttttgac
     1981 aggactgcaa ccggacagag actataaaaa cagtcactta cggacactga ccagacagta
     2041 agcggaccag aaaaagacaa aaatatacac gcaaaatgca cgtgcatttc aaaaaagcct
     2101 ctgattatga agtcagaggc ttttctagtt gtaaataagg gtaacaattt aacacgatgc
     2161 agcttaccaa ttacgtaaca tcaaacgctc taattgttcc caaatcctcc agaccactca
     2221 gctcctcttt catttcacgc tgacggcgat aaatctcatc gttgcgatcg acctgcgcct
     2281 gcaccattgc tgccgccagt tcttccagtt ccggcatcga cagtttcacc tgctgattat
     2341 cggcatcacc ccacgccata tgcgtttgtg ctgtgacaga ttttgccagc atgactaccg
     2401 gggacaggcg gcccagtgag tcggggccag cattccagat acgacc
//
LOCUS       DAEEKQ010000325         2439 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.331, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000325 DAEEKQ010000000
VERSION     DAEEKQ010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.331"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            59..1159
                     /gene="ompC"
                     /locus_tag="KLK57_004331"
     CDS             59..1159
                     /gene="ompC"
                     /locus_tag="KLK57_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HBE0894518.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNERTDNSHLYGADYTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYAETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYGDQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            1323..1805
                     /locus_tag="KLK57_004332"
                     /pseudo
     CDS             1323..1805
                     /locus_tag="KLK57_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1920..>2439
                     /locus_tag="KLK57_004333"
     CDS             1920..>2439
                     /locus_tag="KLK57_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HBE0894519.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLS"
BASE COUNT          798 a          490 c          452 g          699 t
ORIGIN      
        1 cgccgtttaa aattcgttta gaaaatacgt ttaacgataa ttaaaaggat tctgttaaat
       61 gaaattaaaa atagttgcgg tggttgtaac tggtttgtta gctgcgaacg tagcacacgc
      121 tgccgaagtc tataacaagg atggtaataa actcgacctt tatggcaagg ttaccgctct
      181 acgttatttt actgatgata agcgtgacga tggtgataaa acttatgccc gtctcggctt
      241 taaaggagaa acgcaaatca atgatcaaat gattggtttt ggtcactggg aatatgattt
      301 taaaggctat aacgatgaag ccaacggctc gcgcggcaac aagacccgtc tggcctatgc
      361 tggtttaaaa attagtgaat ttggctctct ggactatggt cgtaactacg gtgtcggcta
      421 cgacattggt tcatggaccg atatgttgcc agaatttggt ggcgatacct ggagtcagaa
      481 agatgtcttc atgacatacc gtaccaccgg tctagcaacc tatcgcaact acgatttctt
      541 tggcttaatt gaagggctga actttgccgc gcaatatcaa ggcaaaaatg aacgtactga
      601 caacagtcat ctttatggtg ctgactacac gcgtgccaac ggtgacggtt tcggtatctc
      661 ctcaacttat gtttatgatg gctttggtat cggtgcggta tataccaaat ccgatcggac
      721 aaatgcgcag gaaagagccg ctgctaatcc tctcaatgcc tccggtaaga atgcagaact
      781 atgggccaca ggtataaagt atgatgccaa caacatctac tttgcagcta attacgctga
      841 aacattaaac atgaccacct atggggatgg ttatatctct aacaaagcac aaagttttga
      901 agtggtggcg caatatcaat tcgacttcgg cttgcgccca tcactcgctt acctgaaatc
      961 gaaaggcaga gatctgggcc gctacggcga ccaggacatg attgagtata tcgacgttgg
     1021 tgcgacgtat ttcttcaaca aaaatatgtc gacctatgtt gattataaaa tcaacctgat
     1081 tgatgaaagc gactttaccc gtgccgtaga tattcggacc gataacatcg tcgctacggg
     1141 cattacctat cagttctaac gtcctctcct gaccagccct tctctgggct ggtttttgat
     1201 ttaagtgctg ctgtatgact ccttaagtaa aaatgagcaa tttttgatat ttctggtcat
     1261 tcacctcaat taatattatt ccatatcctc cggggacatc ttactataat atttattgat
     1321 gagtgattat gaccaatatt aatacagctt gtgtaaaaaa taatgccagt tatcaactta
     1381 ataacgcatt acctaacaaa gaaaccatct ccagcaattt ttgtgaacga ctagaacaat
     1441 ggggtaataa atcggttaat aatggtgaag aaagagcaat tgccgtggag cgaattaaag
     1501 aagcttacaa ttcgaacatg gcatctcttg atttgtcata tcttgatttg agtgaattac
     1561 cgcctatccc ctcgacagtt aatacgttaa atctggaaaa caattgtcta acttgtcttg
     1621 acttagctga caatgccagt ctcgttaata tcaacctcag cgttaataaa attaacacga
     1681 taacctttcc aaaagaatca aaactggaaa acatttatat tgatcataac aatctggaaa
     1741 gtttagattt aaaaaatcag cattcattgg ttaatctgga agcgcaaaat aacaaactga
     1801 caaaaattaa tatttctgat agttataaac tgaaatttct taatcttgat tataacaagc
     1861 tagcatcgct ggatgtctcc cggcaagaat ctctgattga gttaagtgcc catcacaata
     1921 tgatcactga ccttatatta cacaatcatc ccaggatgaa aacaatcact ttaaacgaca
     1981 accatattgc acatttaaac gccaaaaaca ctacaaaact ggaatattta aacttaagca
     2041 ataacaattt actgccaacc aatgacattg atcaactaat atcatcaaaa catctttggc
     2101 atgtattagt taacggcatc aacaatgatc cacttgcaca aatgcagtac tggactgcag
     2161 taagaaatat aattgatgac actaatgaag tgaccattga tttatcagga cttaatttaa
     2221 ccactcaacc accagggctg caaaacttca catctatcaa tcttgataat aaccaactca
     2281 cacattttga tgcaaccaac tacgatagac tcgtaaagct aagtctgaat agtaatgctc
     2341 ttgagtcaat aaattttcct caaggcagaa atgtaagtat tacacatata tctatgaata
     2401 ataatgctct cagaaatatt gatatagata ggctttcat
//
LOCUS       DAEEKQ010000326         2437 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.332, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000326 DAEEKQ010000000
VERSION     DAEEKQ010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2437
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.332"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(442..518)
                     /locus_tag="KLK57_004334"
     tRNA            complement(442..518)
                     /locus_tag="KLK57_004334"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(482..484),aa:Arg,seq:tct)
     gene            complement(532..608)
                     /locus_tag="KLK57_004335"
     tRNA            complement(532..608)
                     /locus_tag="KLK57_004335"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(572..574),aa:Arg,seq:tcg)
     gene            complement(616..691)
                     /locus_tag="KLK57_004336"
     tRNA            complement(616..691)
                     /locus_tag="KLK57_004336"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(656..658),aa:Ile,seq:cat)
     gene            complement(732..1790)
                     /locus_tag="KLK57_004337"
     CDS             complement(732..1790)
                     /locus_tag="KLK57_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HBE0894520.1"
                     /translation="MLNTVKISSCELINADCLEFIRSLPENSVDLIVTDPPYFKVKPE
                     GWDNQWKGDDDYLKWLDQCLAQFWRVLKPAGSLYLFCGHRLASDIEIMMRERFSVLNH
                     IIWAKPSGRWNGCNKESLRAYFPATERILFAEHYQGPYRPKDAGYAAKGSALKQHVMA
                     PLISYFRDARAALGITAKQIADATGKKNMVSHWFSAGQWQLPNESDYLKLQALFARVA
                     EEKHRRGELEKLHHQLVDTYTSLNRQYAELLSEYKHLRRYFGVTVQVPYTDVWTHKPV
                     QFYPGKHPCEKPAEMLQQIISASSRPGDLIADFFMGLGSTVKAALALGRRAIGVELET
                     ERFEQTVREVQDLVSQNG"
     gene            complement(1898..2138)
                     /locus_tag="KLK57_004338"
                     /pseudo
     CDS             complement(1898..2138)
                     /locus_tag="KLK57_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309113.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2363..>2437)
                     /locus_tag="KLK57_004339"
     CDS             complement(2363..>2437)
                     /locus_tag="KLK57_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HBE0894521.1"
                     /translation="YDALVTRFDIEEAWAERQLKKVTR"
BASE COUNT          585 a          698 c          560 g          594 t
ORIGIN      
        1 tagcggtcat gtgatgccgg tttcccggta actcagcacc ggtatctgag tcaacgtttt
       61 ctctactggg tcatttccga tacgccctgc ctgctgacag gctttcatca catctgaaaa
      121 tatagcaccc tgactgatac tgtagtacct aaggttccag aaactgtgat gtatccggca
      181 cagaaaagcc cctccggaga gggctggaga gtggcgctat gtgccattgc atgatgccgg
      241 gtgcctcccg gtgaattcag taccagcacc tgaatccgcg attatcccat atacctactc
      301 gctgattgcc cctccgcaca gggggattca ccatgccagt ttcttttaac aaactccccg
      361 caaaacagac aactgtcaac cgtctgaatt gtgagacatt taaaaaaaag gcccgcaaaa
      421 gcgagccagg gaaaataagt gtggcgcgtt gtactggatt cgaaccagtg accgattgct
      481 tagaaggcaa ttgctctgtc cggctgagct aacaacgcat gatgcagata atggaccgcc
      541 atcggggact tgaaccccgc gcagccagct tcgaaggctg gcgctctttc ctgatgagct
      601 aatggcggta tgtgatggtg gcccttgctg gatttgaacc agcgacctgg cgattatgag
      661 tcgctcgctc tcaccactga gctaaagggc cgggagccgc ataataacga cgcgtaatta
      721 attcttcaat atcatccgtt ctggctgact aaatcctgta cttcccgaac cgtctgctca
      781 aaacgttcag tctccagctc aacgccaatt gcacgacgcc cgagcgccag tgccgctttc
      841 actgtcgaac ccaaccccat gaaaaaatct gcaatcaggt cacccggacg actgctagcg
      901 ctgattatct gctgcagcat ttctgccggt ttttcgcacg gatgtttccc gggatagaac
      961 tgcaccggtt tatgcgtcca cacatcggta tacggcacct gcaccgtcac gccaaaatac
     1021 cgccgcagat gtttatattc actcagcagc tccgcatact gccggttcag tgaggtatac
     1081 gtatccacca gctggtggtg gagcttttcc agttcaccac gccgatgctt ctcttctgcc
     1141 acccgggcaa acagcgcctg taatttcaga taatcgcttt cattcggcaa ctgccactga
     1201 ccggcactga accagtgcga caccatgttt ttctttcctg tggcatctgc aatctgtttt
     1261 gccgttatcc ccagggcagc tcgcgcatca cgaaagtaag aaatcagcgg ggccatcaca
     1321 tgctgtttca gtgcactgcc cttcgccgca tacccggcat ctttcggacg atacggcccc
     1381 tgataatgtt ccgcgaacag aatgcgctct gtggcgggga aatacgcccg caggctttcc
     1441 ttgttgcatc cgttccagcg tccggacggc ttcgcccaga taatatggtt cagcacactg
     1501 aagcgttcac gcatcatgat ttcgatatca gatgccaggc gatgaccaca gaacaggtaa
     1561 agacttccgg caggtttcag cacccgccag aactgcgcca gacactggtc cagccacttc
     1621 aggtaatcat cgtcgccctt ccactggtta tcccagccct caggcttcac tttaaagtac
     1681 ggcgggtccg tgactatcag gtcaacagaa ttttcgggta acgaccggat aaattccagg
     1741 cagtcggcgt tgattaactc acaactggat atttttacag tattaagcat ggatcattaa
     1801 gcctgtctct gataggctca ttctgctttt gcgcaaagca gtgggcctga ggtttgcttg
     1861 tgaacccaac gcatgagcag atggctggtg ggtgccccta acacccacca gccgcccatt
     1921 taccacaaat aaaaaagcct tcactgcgga aggcgtctgt aacaaccgaa ctgatagtct
     1981 gccagacccg ccataaccag ctgggtcagt attaactggc agcgttcgcg tgaaaggtaa
     2041 gtattctgcg ctatctcccc gactgtcgcc ggttcggtaa cgcttaattc attaaacacc
     2101 actctggcgg tttctgtcat atcctgctgt tttagcatgt cttttccttt tccggttaac
     2161 gtgacacacc aataactctt gtcgaaaaag ccagcaagct gaaagacagg tattcaccgc
     2221 caccagcgcg tttactgtac tgacgcgatt tcagtcataa aaaacccgct cggcggcggg
     2281 ttgtagaaac tcttctaacg tcaggcataa aacgcccatc gttatgacga atttaccaca
     2341 gattccggaa aaatcaacct tgttacctag ttaccttttt taactgccgc tcagcccatg
     2401 cttcttcaat atcaaaccgg gtcaccagcg catcata
//
LOCUS       DAEEKQ010000327         2403 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.333, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000327 DAEEKQ010000000
VERSION     DAEEKQ010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.333"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            375..641
                     /gene="hipB"
                     /locus_tag="KLK57_004340"
     CDS             375..641
                     /gene="hipB"
                     /locus_tag="KLK57_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HBE0894522.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENNPDNTTLTTFFKILQSLELSMTLCDAKNASPEATEQQDLEW"
     gene            641..1962
                     /gene="hipA"
                     /locus_tag="KLK57_004341"
                     /pseudo
     CDS             641..1962
                     /gene="hipA"
                     /locus_tag="KLK57_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
     gene            2146..2247
                     /locus_tag="KLK57_004342"
                     /pseudo
     CDS             2146..2247
                     /locus_tag="KLK57_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          667 a          504 c          618 g          614 t
ORIGIN      
        1 agatttggcg cagtggagta gcaacgcgca ggatcgctgg catcttggcg tgatggcagg
       61 ctacgccaat cagcattagc ctgtggggga atgtcggtgt gcaactaggt gataaaggct
      121 atagcgatac tcagggcatg ctgggagtga aatatagctg gtaaaccgta taagccgcat
      181 gtcgagatgg catgcggctt aatattgcca acttcaaaag gcttttaaag cctccctttt
      241 atccgcgatc gcggatatcg cagcgtttat cccgtacagc ggataagatg tgtttccaga
      301 ttgactttat cctcactaaa ggataaaact gataatatcc ccttaagcgg ataaatttgc
      361 tgtggacgta tgacatgatg agctttcaga agatctatag cccaacgcaa ttggcgaatg
      421 caatgaaact ggttcgccag caaaatggct ggacgcagag cgagctggcg aaaaaaattg
      481 gcattaagca ggcgacgatt tccaatttcg aaaacaaccc tgacaatacc acgctgacga
      541 ccttttttaa gattttacag tcgcttgaac tctcaatgac gctatgcgac gcgaaaaatg
      601 cctcgccaga agcaacagaa cagcaagatc tggagtggta atgcctaaac ttgtcacttg
      661 gatgaacaac cagcgggtag gcgagttaac gaagttagcc aacggcgcgc acacctttaa
      721 gtatgcaccg gagtggttag ccagccgtta tgccagaccg ttgtcacttt cgctgccatt
      781 gcagaggggg aatatcacct ctgatgccgt atttaacttc ttcgataacc tgttacccga
      841 tagcccgatt gtacgtgacc ggatcgttaa acgttatcat gccaaatcca gacaaccgtt
      901 tgatttgttg tcagaaatag ggcgagacag cgttggtgcc gtgacgttaa tacccgaaga
      961 cgaaaccgta acgcgtccga taatggcatg ggaaaagctt actgaagcca gacttgaaga
     1021 agtattaacg gcttataaag cagatatccc gctaggcatg attagagaag aaaatgactt
     1081 tcgcatctcg gttgctggcg cacaggagaa gacagcactg ctcagaatag gcaatgactg
     1141 gtgcattccg aaaggaataa cgccgacgac gcacatcatt aaattaccga ttggcgaaat
     1201 caggcagtcc aatgcgacgc tcgatctcag ccaaagcgtt gataatgagt attactgtct
     1261 gctgctggcg aaagaacttg ggttgaatgt tccggacgca gaaatcatta aagcgggaag
     1321 ggtgcgcgcg ttagcggtcg aacgttttga caggcgttgg aatactgagc gaacggtttt
     1381 acttcgcttg ccacaggagg atatgtgtca gacattcggt ttaccttcat cggtgaaata
     1441 tgaatcagat ggaggcccag gcatcgcgcg gatcatggcg tttttgatgg ggtccagcga
     1501 ggcgctgaaa gatcgctatg attttatgaa gtttcaagtg ttccagtggt tgattggcgc
     1561 aacggatggc catgcaaaaa acttctccgt atttattcag gctggcggca gttatcgact
     1621 cacgccattt tacgacatca tttcagcatt tccggtcctt ggcggtacgg gaataacatc
     1681 agcgatctca aactggcaat ggggcttaac gcatccaaag gcaaaaaaac ggcaatcgat
     1741 aaaatttatc cgcgacattt tttggcgaca gcaaaggtgc tgagattccc ggaagtgcag
     1801 atgcatgaaa tcctaagtga ctttgccaga atgattccgg cagcactgga taacgtgaag
     1861 acttcattac cgacagattt tccggagaac gtggtgacgg cagttgaaac caatgtgttg
     1921 aggttgcatg gacggttaag ccgagaatac ggtagtaagt gagatatggg gtttggtcgt
     1981 tgttaatgag caatcatgac cggccaacga ctggattctc ccttggttta tgtttgtgcg
     2041 cataatgtat attatgttaa atcatgtctg agtgatgcga agtgcttttc tggtagtcgt
     2101 tattcgttca gattaggtgt ccttgtgatt taaagatgtt atcgtggcac gaatatactg
     2161 ccattccagg ctcgttatat tgctctcggg cagtccgtag ctggtactgc aaacgctgat
     2221 gcgaccttca aagttcaata tctataattc aatcttaagg tggctatacg tcatctgaaa
     2281 ttgaaacagg gtgtgtgtgg catggctacg cacacccgag caattcacca atgtgaatct
     2341 cattattaat gaggagtggt ttgtatttgg agcgctatga aaaatcacat aatatctaaa
     2401 cta
//
LOCUS       DAEEKQ010000328         2342 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.334, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000328 DAEEKQ010000000
VERSION     DAEEKQ010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2342
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.334"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..956)
                     /locus_tag="KLK57_004343"
     CDS             complement(<1..956)
                     /locus_tag="KLK57_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HBE0894523.1"
                     /translation="MDLLAESITEVAVSGEIANTDRVLNIAYGIDRNFLFGAAVSMQS
                     VVMHNPDLAVKFHLFTDYIDEDYLQRVNAFTSKNANVEVRIYKVSSAFIDIFPSLKQW
                     SYATFFRLVAFQYLSETIENLLYIDADVICKGSLAGLLDINFDGDKFAAVIKDVPFMQ
                     EKPAKRLAIEGLPGNYFNAGVVYLQLEAWAKNDFMNKAIAMLASDPQHTKYKCLDQDI
                     LNILFFGHCIFISGDYDCFYGIDYELKNKSDEDYKKTITDDTKLIHYVGVTKPWNDWT
                     NYPCQKYFNEAYQASCWNDVAFIPATNEKQYQVKYQHAKKNG"
     gene            complement(982..1674)
                     /gene="rfaY"
                     /locus_tag="KLK57_004344"
     CDS             complement(982..1674)
                     /gene="rfaY"
                     /locus_tag="KLK57_004344"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HBE0894524.1"
                     /translation="MITSIRYRGFSFYYKDNDNKYKEIFDEILAYNFKTVKVLRNIND
                     TKVSLIDTKYCRYVFKVFAPKTKRNERFLKSFVKGDYYQNLIVETDRVRSAGLTFPND
                     FYFLAERKIFNYASVFIMLIEYVEGVELNDMPIIPEDVKAEIKASMEKLHALNMLSGD
                     PHRGNFIVSKDGVRIIDLSGKSCTAERKARDRLAMERHLGIANEIKDYGYYSVIYRTK
                     LRKFIKKLKGKA"
     gene            complement(1684..>2342)
                     /locus_tag="KLK57_004345"
     CDS             complement(1684..>2342)
                     /locus_tag="KLK57_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001001722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lipopolysaccharide 1,2-glucosyltransferase"
                     /protein_id="HBE0894525.1"
                     /translation="LSESISTLLYLDADVVCKGSLKPLTEIIFKDEFAAVIPDNVSTQ
                     AACAKRLNIPEMNGRYFNAGVIYVNIKKWHEANLTPYLLTLLRGETKYGSLKYLDQDA
                     LNIAFNMNNIYLAKDFDTIYTLKNELHDRSHRKYQQTITDKTVLIHYTGITKPWHSWA
                     GYPSASYFNIAREQSPWKKYPLKEARTVAEMQKQYKHLFAHGEYIKGITSLIKYKLKK
                     "
BASE COUNT          716 a          471 c          406 g          749 t
ORIGIN      
        1 tcaccatttt tctttgcatg ttgatatttc acttgatact gcttttcatt cgtggctgga
       61 ataaacgcca catcattcca gcaagaagcc tgataagcct cattaaaata cttctggcag
      121 ggataattcg tccagtcgtt ccagggcttc gttacgccaa cataatgaat cagcttagta
      181 tcatcggtaa tggtcttttt ataatcttca tcgcttttat tttttaactc ataatcgatg
      241 ccataaaagc aatcataatc gccgctaata aaaatacaat gaccaaagaa cagaatattt
      301 aaaatatcct gatcaaggca tttatacttc gtgtgctgcg ggtcacttgc cagcatagca
      361 atggctttat tcataaaatc atttttcgcc catgcttcaa gctgcagata tactacaccg
      421 gcgttgaaat aattccctgg aagtccctct atagccagac gcttcgctgg tttttcctgc
      481 ataaaaggca catctttaat aacagctgca aacttatccc catcaaaatt aatatcaagc
      541 aatccagcta atgagccttt acagatgaca tcagcatcga tatataacag attttcaata
      601 gtttcactca gatactggaa cgcaactaaa cggaagaatg ttgcataaga ccactgtttc
      661 aggctgggga agatatcaat aaaggcactg gagactttat aaattcttac ttcaacgtta
      721 gcatttttgc tggtaaaagc attaacacgt tgtagataat cttcatcaat gtagtcggta
      781 aagagatgaa acttaaccgc aaggtccgga ttatgcataa caactgattg catagatact
      841 gccgcaccaa ataaaaagtt acggtcaata ccgtaagcga tatttaacac acgatcggtg
      901 ttagcaattt ccccagagac agcgacttca gtaatactct cagctaataa atccatgttg
      961 gttccgtttt gactgtgtgg tttacgcttt gccttttaat tttttaataa atttgcgtag
     1021 tttggttctg tagattactg aatagtagcc ataatctttt atttcgttgg caatacccag
     1081 gtgacgttcc atggccagac gatcacgagc tttacgctct gccgtacagc ttttaccgga
     1141 taggtcaatt atcctgacac catctttact aacgataaag ttaccccggt gtgggtcgcc
     1201 cgatagcata tttaaggcat gcaatttttc cattgaagcc ttaatttctg ctttaacatc
     1261 ttctggaatg atcggcatat cattgagctc tacgccctcg acatactcga taagcatgat
     1321 aaaaacactg gcatagttga aaattttacg ctcagccaga aaataaaaat cattaggaaa
     1381 agtaagacct gcacttctga cacgatcggt ttcaacgatc aaattctgat agtagtcacc
     1441 ttttacaaaa gattttaaaa aacgttcatt ccttttcgtt ttcggggcaa agactttaaa
     1501 aacatagcga caatatttcg tatcaatcaa tgatactttg gtatcattaa tattacgtaa
     1561 tacttttact gttttaaaat tataggccag tatttcatca aagatttctt tatatttatt
     1621 atcattatct ttgtaataaa atgagaagcc gcgatagcgt atacttgtaa tcataagagt
     1681 aatttatttc ttaagcttgt acttaattaa tgaagttatg cctttaatat actcaccatg
     1741 ggcaaacaga tgcttatatt gtttctgcat ttctgcaaca gtccgcgcct ctttaagagg
     1801 atatttcttc cagggagatt gttcacgcgc gatattaaag tatgatgcag acggataacc
     1861 agcccagcta tgccatggtt tagttatccc tgtatagtga atcaatactg ttttatcggt
     1921 aatggtttgc tgatactttc gatgactacg atcatgaagt tcgtttttca gggtataaat
     1981 agtatcaaaa tccttcgcga ggtagatatt attcatatta aatgcgatat tcaacgcatc
     2041 ctgatctaaa tatttaagag agccatattt agtttcccct cgtaaaagcg tgagtaaata
     2101 cggtgtcaaa tttgcttcat gccatttttt aatattgaca tagataacgc ctgcattgaa
     2161 ataacgtcca ttcatctcgg gaatgttgag gcgttttgca catgccgcct gagtactaac
     2221 attgtcagga atgaccgcag caaactcatc tttaaatata atttctgtga gaggcttcag
     2281 acttccttta caaacgacat cggcatccag atacagcagt gtggaaatac tttcactcag
     2341 at
//
LOCUS       DAEEKQ010000329         2334 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.335, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000329 DAEEKQ010000000
VERSION     DAEEKQ010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.335"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(1420..1971)
                     /locus_tag="KLK57_004346"
     CDS             complement(1420..1971)
                     /locus_tag="KLK57_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0894526.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFTDEAVSGSVK
                     ASLRNGFSRLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQTLISQ
                     GKTRLQIQEELGISRATYYRLAK"
BASE COUNT          662 a          500 c          438 g          734 t
ORIGIN      
        1 tggctatgta tttggtcgca tattttgcat tgcacacaga cctcttcaat cgccctgtaa
       61 cgagttaaac gacctcttca ggtcaactca taaggtcagg tactttttgt gtcagaatgc
      121 gattgagagc gtcttacaag gaaaactaat ggtaaatctc cctcctgagc tggggtcacc
      181 tgatgtatgg actggtggag gtattgggct gattgcaggc ctgctacttc gctacgtcct
      241 cgtcagagtg cttgttgtct ttaaactgtt cggtcgtaca tggacgcttg accttaagga
      301 taacgaccac acagaccccc gttaaactcc ttcaaaaccc ctgcacgaga tgacagatag
      361 aagtctatgg tacaggggtg tctttagcat tttgtgctat ctattaaaaa aatagcttga
      421 cagaaccgga aagccgtatc tatcaagcat tcacctctct gacagtaatt tctaaaagtt
      481 cttatttttc aataagttgt aattagccat atatagagaa ctaaaagatt ttttagctat
      541 tttgtttaaa agcatatcaa acgatacatt ttattgattt aggtcaaaga caaaagcatt
      601 gacaaaatgg taagattgtt cacatgacct tacttaacag acttttaaga gactttgaaa
      661 cagcctgttt aacaggtcac tacctctggt cttatcgttc ccttctttct aggtggttat
      721 ctgttaagag tttttcagtt tttattctta agataattgt taagatgata ctctcccttc
      781 gggttatatc gtatctcttc tcccgttaaa tatcccttca cagtcagact aaataacacc
      841 tgtgtagttc ttggtagaat ctttcaaggt aacttagctc tgagtttatg aagttgacct
      901 atgagataga ctctaccgaa tctgttagtc aattgttgct taggtgctgc taaggcttac
      961 ttagtggaaa gagaggggat atgtgaccat aggggaaccc ctcctgttaa aagactactt
     1021 agaagtctga ggttttagta aaatctgtga atatggcttc tcagacgctc tgtaacgcat
     1081 tctaagcgat tttacccctt tattttaagg tggttagtac atccctatag gttaccccta
     1141 aaaatggggt taaaactcgc tacagagctt tctatgagat tagcttctaa tcgaatcaac
     1201 tatgaggttt ggtaggattg tgcaaaatct gtacagtaac ttttcagaat ctgaaaattt
     1261 ttataaaaaa tgactagccc tctttgaatc tcatcttccc cgaatcttca aaaatatctg
     1321 acagatgtct gtagctgcta agtacccgtc acaccagcta tgtgaccccc ttggcaaaaa
     1381 agccaaatat cagctacatc catgtaacta ttcagtaact tatttagcaa gtctgtaata
     1441 agttgctcta gagattccta actcttcctg aatctgcaac ctcgtcttac cttgtgaaat
     1501 aagcgtctgt acagcctctg aagaagcttt tacaggcctc cccatatgta ccccttcagc
     1561 tttagcacgt tcaataccag cctttctacg ctctgctatc aagcttcttt caagctgtgc
     1621 caacgttgac ataatgccta acattgcttc acccatagcg cttgataagt caaacccttc
     1681 tctaaggctg ataactgtca cacctttagc ctgtagcgct ttaaccgttg ataacacatc
     1741 aattgtgtca cgtccaagac ggtctacagc taccacaact acggtgtcac cttctcgtgc
     1801 ataagctaat agtctgctga aaccgtttct taaagaagct ttgacagaac cactgacagc
     1861 ttcatcagtg aaccatttag caatgttgta ccctgcctct tcaatttgca tttgctggtt
     1921 agtgatggat tggtcatctg tactcaccct gcaatatcca agtatcgcca taatgttccc
     1981 cttagaagtc tcaaaagtta gtctcataag tttagctgtc tcataatcca aaaacaacct
     2041 tttgagacta cttgagggtg cattctaccg ttacgttgaa agtgtctcaa aaggaagtcc
     2101 ttttaagact ggttatgaag gagctatgag gttgattagg ggttggtaca agcccaacta
     2161 accgaatcaa ctatgaagat tttaggaggg taactgtaac catcactttc taactttcac
     2221 gtaggaaatt gttaagtggt ttgatagtta caccatgtga ccaagcatct atatatcgtt
     2281 atatagccct gtatatagca ctatatgtag tgtcgagaga ggaacgacta acac
//
LOCUS       DAEEKQ010000330         2330 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.336, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000330 DAEEKQ010000000
VERSION     DAEEKQ010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2330
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.336"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..771
                     /gene="nirC"
                     /locus_tag="KLK57_004347"
     CDS             <1..771
                     /gene="nirC"
                     /locus_tag="KLK57_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HBE0894527.1"
                     /translation="AARIARLSANNPLGFWVSSAMAGAYVGLGIILIFTLGNLLDPSV
                     RPLVMGATFGIALTLVIIAGSELFTGHAMFLTFGVKAGSISHGQMWAILPQTWLGNLV
                     GSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALCNWLVCLAIWMAL
                     RTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYTLAGIGHNLLWVT
                     LGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            790..2163
                     /gene="cobA"
                     /locus_tag="KLK57_004348"
     CDS             790..2163
                     /gene="cobA"
                     /locus_tag="KLK57_004348"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HBE0894528.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT          510 a          593 c          652 g          575 t
ORIGIN      
        1 gctgcgcgca ttgcacgcct gtcggcaaat aacccgctcg gcttttgggt cagctccgcc
       61 atggcgggcg catatgtggg tcttgggatc atcctgattt tcacgctcgg taatttgctc
      121 gacccgtccg tacgtccgct ggtgatgggc gcgacctttg gtatcgcctt aacgctggtg
      181 attatcgccg gttctgaact gttcaccgga cacgccatgt tcctcacctt tggggtaaaa
      241 gcgggcagca tcagccacgg gcaaatgtgg gcaatcctgc cgcaaacctg gctgggtaac
      301 ctggtcggtt ccgtcttcgt tgccatgctc tatagctggg gcggcggtag cctgctgccg
      361 gtagatacca gcatcgttca ctccgtcgcg ctggctaaaa ccactgcacc ggcaatggta
      421 ctgttcttca aaggtgcatt gtgtaactgg ctggtttgcc tggctatctg gatggcgctg
      481 cgcactgaag gggcggcgaa atttatcgct atctggtggt gtctgctggc atttatcgcg
      541 tccggctacg agcactccat cgctaacatg acgctgttcg ccctctcctg gttcggcaac
      601 cacagcgaag cctacacgct ggcgggtatt ggtcataacc tgctgtgggt gacgctgggt
      661 aatactttat cgggtgccgt attcatggga ttgggttatt ggtatgctac gccgaaagcg
      721 aatcgtccgg ttgcggacaa atttaatcaa actgaaacgg ctgccggtta attactaagg
      781 ggtttttacg tggatcattt gcctatattt tgccaattac gcgatcgcga ctgtctgatt
      841 gtcggcggtg gtgatgtcgc ggaacgcaaa gcaaggttgc tgttagacgc aggcgctcgc
      901 ttaacggtga atgcattagc gtttattcca cagttcaccg catgggcaga tgcaggcatg
      961 ttaaccctcg tcgaagggcc atttgatgaa agccttctcg acacctgctg gctggcgatt
     1021 gcagcgacgg atgatgacac gcttaaccag cgcgtcagcg aagccgctga agctcgtcgc
     1081 atcttctgta acgtagtcga tgcgccgaaa gccgccagct ttattatgcc gtcgattatt
     1141 gaccgctcac cgctaatggt agcggtctcc tctggcggca cctctccggt tctggcgcgc
     1201 ctgttgcgcg aaaaactcga atcactgctg ccgttgcatc tgggccaggt ggcgaaatat
     1261 gccgggcaat tacgcgggcg agtgaaacaa cagttcgcca cgatgagtga gcgtcgccgt
     1321 ttctgggaga aattgttcgt taacgaccgc ctggcgcagt cgctggcaaa caacgatcag
     1381 aaagccatta ctgaaacgac cgaacagtta atcaacgaac cgctcgacca tcgcggtgaa
     1441 gtggtgctgg ttggtgcagg tccgggcgat gccgggctgc tgacgctgaa aggactgcaa
     1501 caaattcagc aggcagatgt ggtggtctac gaccgtctgg tttctgacga tattatgaat
     1561 ctggtacgcc gcgatgctga tcgcgttttc gtcggcaaac gcgcgggata ccactgtgta
     1621 ccgcaggaag agattaacca gatcctgctg cgggaagcgc aaaaaggcaa acgcgtggtg
     1681 cggctgaaag gtggcgatcc gtttattttt ggccgtggtg gcgaagagct ggaaacactg
     1741 tgcaacgcgg gtattccgtt ctcggtggtt ccgggtatta ccgcagcttc tggttgctct
     1801 gcctattcgg gtattccact cacgcatcgc gattatgccc agagcgtacg cttaattacc
     1861 ggacacttaa aaaccggtgg cgagctggac tgggaaaacc tggcggcaga aaaacagacg
     1921 ctggtgttct atatgggatt gaatcaggcc gcgactattc agcaaaagct gattgaacac
     1981 ggtatgcctg gcgaaatgcc ggtggcaatt gtcgaaaacg gaacggcagt cacgcagcgc
     2041 gtgattgacg gtacgctcac gcaactcggt gaacttgctc agcaaatgaa cagtccatcg
     2101 ctaattatta ttggtcgggt tgttggcctg cgcgataaac tgaactggtt ctccaaccat
     2161 taatttaacc cgggccagag aattctggcc ttcttaatga ctctttttat atattcagca
     2221 aataaaacat aaccccttaa taaatattat ctgctaatgg gttatattca ccgttacagc
     2281 gacttacaaa attaagtcat tcgctcactg acgccaatta aatattcctg
//
LOCUS       DAEEKQ010000331         2329 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.338, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000331 DAEEKQ010000000
VERSION     DAEEKQ010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.338"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            20..238
                     /locus_tag="KLK57_004349"
     CDS             20..238
                     /locus_tag="KLK57_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HBE0894529.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPLIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            240..605
                     /locus_tag="KLK57_004350"
     CDS             240..605
                     /locus_tag="KLK57_004350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE0894530.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPACHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEKYQEGATA"
     gene            602..1267
                     /locus_tag="KLK57_004351"
     CDS             602..1267
                     /locus_tag="KLK57_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894531.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFDKAEVECWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1267..1632
                     /locus_tag="KLK57_004352"
     CDS             1267..1632
                     /locus_tag="KLK57_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HBE0894532.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVAPAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
     gene            2087..2242
                     /locus_tag="KLK57_004353"
     CDS             2087..2242
                     /locus_tag="KLK57_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HBE0894533.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRIGQTEVAVF
                     VDYESRE"
BASE COUNT          627 a          496 c          628 g          578 t
ORIGIN      
        1 cccgaaaggg cggtaatgaa tggttacatt atttagaaaa aaatatccgc gaaagagtag
       61 aacaacagaa ttcctgtttc tcattctgtt tatcgtgttg atgacaccga tatccccgct
      121 aatttttgtc tggataatcg gaaaaataat tgagccagtt attgaattgt ataacgacgt
      181 ggtatgggcg tcattcaaca cactgcacaa taaaattaat ccgtataagg aaagctgata
      241 tggcactgac gaaaaaacaa cgtgcagaac tgcgcatgaa gttcggtggt cgctgcgctt
      301 attgcggctg cgaacttggc gaaaagtggc atgcagacca tgtaaaaccg gtcattcgtt
      361 ttgatggaaa tatgcttcac caggaacgtg acgatatatc caacatggta ccagcatgcc
      421 acccatgcaa tctgcacaag cattgcagta gcctggaaga ttatcgacga attatcagtg
      481 atggtcgtcg tgaattcctt gcgtccggga aaggcaaagc gctggttcgt atgggattgg
      541 ttgaaatgaa atctgacccg gttgtgttct ggtttgaaaa atatcaagaa ggggctacgg
      601 catgaccact attaccaaag agcgactgca atggctggct aacatttctg gccgcgatga
      661 tattgacgat atagacggcg gtgaaattcg tgaactggcg cttatcgctc tggcatcact
      721 ggaagcagag cctgtagcgg aatgcattgt tgaagatggg ggcatgtgta ttgacgggtt
      781 cggtgagtat gtgggtcact cgctgcctga tggaatgcat gagctttatg ctgccccgcg
      841 aatgcgacaa cctgatggat ggaaagccgt agctgtagcg tggaaggtga cgtttactca
      901 agttgaccag gaatctaata cgttcactgc tatatatttt gacaaagcgg aagttgagtg
      961 ttgggtacga ctgcataaag catgtgattt tcgggcagat ataacaccgc tttacgcagc
     1021 gccggcagtg ccggttgcag taaacgacga catggcttac gcattccatc acgcactgtc
     1081 agattcatcg cttggctctg atgaaatcga agaaattaaa accggtttgc gtgctgcctt
     1141 tgccaacgtc actatccaac cagagccgat agtgccggat gaaatcgggc caaacgatag
     1201 taatacgttt gattatgttg atggctggaa cgcctgccgc gctgccatgc ttaagggaga
     1261 taaataatga ttaatcgaac caaactggag cacattctcg aatatgccag gcagcagaaa
     1321 tgtattgggc aactttgtaa aattccacca ggagatatgg ttgaaatcgt ggaaatggcc
     1381 atgcgtaagg ctggcaactc tccggtagct ccggctggct ggataagctg tagtgatgca
     1441 gttcctgctg aatattgcga tgtgattctt ctcgatgatc tcgggaatgt attccccggt
     1501 tcctgggata aggttttttg ccccactcgt ggcgggaata agatggcttt tgtggacaaa
     1561 gacggcgttg aagtagagag ctcaactcac tggatgccgc taccggaacc accgcaggag
     1621 gtgaatcaat gacctggcct gaggcattca ccacggtagg aattgcgatg gcggtggcgc
     1681 tggtggtgta ttcgatttgc cgttggggtt aacaaacaaa aaccccggat tgatggtccg
     1741 gggtttttga aggaaacaaa acagaaacag caattgccgt tacctgttgt taccatggca
     1801 agtaaacgta tctcaggcga gcgcattgcg ccgttctgac gcagataaac tagcctggat
     1861 agatggtgct ggcaataaaa aatagcgttt tcttatcggt gtcggtaaga ttgctgcggg
     1921 tgcttgaggc tgtctgcctc gggtatgtca ctgtaaggca gacagagaaa agccccagtt
     1981 aacattacgc gtcctgcaag acgcttaaca ttaatctgag gcccaatcta tgtctcacaa
     2041 atgtaggtta gcctcttacg tgccgaaagg caaggagaag caggctatga agcagcaaaa
     2101 ggcgatgtta atcgccctga tcgtcatctg tttaaccgtc attgtgacgg cactggtaac
     2161 gaggaaagac ctctgcgagg tacgaatccg aatcggccag acggaggtcg ctgttttcgt
     2221 agactacgaa tctagagagt aagagtgacc aggcgggaga gtaatctccc gccacctttg
     2281 atgtgtcagg catcctcaac gcacccgcac ttaacccgtt tcggcggtt
//
LOCUS       DAEEKQ010000332         2274 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.340, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000332 DAEEKQ010000000
VERSION     DAEEKQ010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2274
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.340"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(136..1140)
                     /gene="argF"
                     /locus_tag="KLK57_004354"
     CDS             complement(136..1140)
                     /gene="argF"
                     /locus_tag="KLK57_004354"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HBE0894534.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKTFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
     gene            1302..1718
                     /gene="rraB"
                     /locus_tag="KLK57_004355"
     CDS             1302..1718
                     /gene="rraB"
                     /locus_tag="KLK57_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HBE0894535.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            complement(1764..2267)
                     /locus_tag="KLK57_004356"
     CDS             complement(1764..2267)
                     /locus_tag="KLK57_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HBE0894536.1"
                     /translation="MNNIAPQSPVMRRLTLLDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYEGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
BASE COUNT          582 a          559 c          553 g          580 t
ORIGIN      
        1 aggaaaatta aatcctccgg aagatttacg tatagcaata aaaaaattgg ggtggaataa
       61 ttgaactatc actgcataaa ataatgtgac gccgggatgt ttttgtattt cccggcatct
      121 ttatagcgat atcaattatt tactgagcgt cgcgaccatc accgctttga tggtatgcat
      181 gcggttttcc gcctgatcaa atacaatgct ggcggcagat tcgaagacct catcagtcac
      241 ttccattccg ccatgtaggc caaactcttc cgccattttt ttgccaagcg tcgtttggtc
      301 gtcatgaaac gcgggcaggc agtggaggaa tttgacctcc gggttaccgg tcaactgcat
      361 catcttgctg ttcacttgat aatcacgcag caatgcaatg cgttcagccc atttctcttt
      421 tgcttcaccc atcgacaccc atacatcggt atagataaag tcagcacctt caactccttt
      481 agctacatct tccgtcagcg taatattccc gccattttgt tgtgccaggg cgcggcattc
      541 cgtaaccagc gcagcttccg gccagcatgc ctgtggcgcg accagacgca aatccagacc
      601 ggtaagcgct gcagcttcga gcatcgaatt gcccatgttg ttacgtgcgt cacctgcata
      661 gactagcgtc atttcgttga acgttttgcc gggcaaatgc tcctgcatgg tgaggagatc
      721 cgccagcagc tgcgtggggt gaaactcatt ggtcaggccg ttccataccg gcacgccagc
      781 gtattccgcc agtgtttcga caatctcctg accatagccg cgatactgaa taccgtcata
      841 catgcgacca agcacgcggg cagtgtcttt aatcgactct ttatgaccaa tttggctgcc
      901 gcttgggccg agataagtaa cacgagcccc ctggtcatat gcggcaactt cgaaagagca
      961 tcgggtacgg gtcgagtctt tttcgaagat gagcgcgatg tttttaccag tgagtttggc
     1021 ttcttcttta ccgcttttct tatcggcttt cagcttcgcg gctaactgca gcaggctgtt
     1081 gagttcagct ggcgtgaaat cgagtaattt caggaaatgc ttatgataaa acccggacat
     1141 aggtccctcc tgtggctaac gccttaatga attaaaattc aatttatatg gatgattatt
     1201 catttgcaag tctaaagcat aaatctttat cacaaaggtg gaggcaatgt cagtggtgtg
     1261 tgacaataag agtatcggca ggacattaag aggaatgagc catggcaaac ccggaacaac
     1321 tggaagaaca gcgtgaagaa acacgtttga ttattgaaga attactggaa gatggcagcg
     1381 atccggacgc gttgtacacc atcgaacacc atctttccgc agacgatctg gaaactctgg
     1441 aaaaagcggc agttgaagcg tttaaactcg gttacgaagt gaccgatcca gaagagctgg
     1501 aagttgaaga tggtgatatc gtgatttgct gcgacatcct cagcgagtgc gcgttgaatg
     1561 ccgatctgat cgatgcccag gttgaacaac tgatgacgct ggcggagaaa tttgacgttg
     1621 agtacgacgg ttggggcact tactttgaag atcctaacgg cgaagatggc gacgatgaag
     1681 attttgtcga tgaagacgat gacggggttc gccactaatt aattgattaa aatgcccgat
     1741 agcacatact agcgggcatt tttttagagt tcgcgtagca tccgcacttc acaatcgaca
     1801 tggcccgtgc agccaagcgc atagtcgata tgctcaaagc ccaaatgctc ataaagcgca
     1861 atggcttcct ttaaaaaagc ggtcgtttcc agatagcagc gtttgaagcc catctctcgt
     1921 gcctgctcca tcgccattaa ggccagtttt ttcgctagcc ctttaccgcg aatagccgga
     1981 agaaaataca tcttttgcag ttcgcaaatg tccgactcac tcccggttaa tggcgcaatc
     2041 ccaccgccgc cgaccacttc accctcgtac tcaaccaccc aatatgcatg gccaggctga
     2101 ctatacactt gatacagctc gtcaagattc ggatcggcga cggtgtagcc tttatcagcg
     2161 gtaagaccgt attcggcgga tacctgacga atgacgcggg cgatagcagg attatccagc
     2221 agcgtcaggc gacgcattac aggtgattgc ggcgcaatgt tattcatagt ttaa
//
LOCUS       DAEEKQ010000333         2183 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.341, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000333 DAEEKQ010000000
VERSION     DAEEKQ010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2183
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.341"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            187..2172
                     /gene="yjcS"
                     /locus_tag="KLK57_004357"
     CDS             187..2172
                     /gene="yjcS"
                     /locus_tag="KLK57_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HBE0894537.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTANAKEEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTIGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
BASE COUNT          587 a          573 c          528 g          495 t
ORIGIN      
        1 tatttacaga gataataaat tcgcgtaatt ttccctcttc agcacaaatg cctaataaaa
       61 tggatcgttc agctcacact tccacttatt aaagtcgcaa attcccaatt atgataaccc
      121 cagctttaaa tgacgtattt tgcatggatt acatatttac gacataaatg ataaatggag
      181 atttacatga ataactctcg gttattccgt ttgagcagaa ttgttattgc gttaactgcc
      241 gccagcggca tgatggtaaa taccgctaac gcgaaagagg aagcgaaagc cgccactcaa
      301 tatacccaac aggttaatca gaattacgcc aaatcattac cgtttagcga tcgtcaggat
      361 tttgacgacg ctcagcgtgg atttatcgct ccgctgctgg atgaaggtat tctgcgcgat
      421 gctaatggca aaccatatta tcgcggggaa gattataaat ttgatatcaa tgcgcccgcg
      481 cccgaaaccg ttaaccctag cctgtggcgt cagtcgcagt taaacggtat ttccggcctg
      541 tttaaagtca ccgacagaat gtaccaggtt cgcggtcagg atatctccaa catcaccttt
      601 atcgaaggtg ataccgggat tattgtcatc gacccgctgg tgacgccacc gagcgcaaaa
      661 gccgcccttg acctttactt ccaaaatcgc ccgcaaaaac cgattgttgc ggttatttat
      721 acccacagcc ataccgacca ctatggcggc gtgaaaggca ttatctctga agctgatgtg
      781 aaatccggta aagtgcaggt tatcgcccct gctggcttta tggacgaagc catcagcgaa
      841 aacgtactgg cgggtaatat tatgagccgc cgtgcacttt actcctacgg cctgctgctg
      901 gcgcataacc ctcagggtaa catcggcaat ggtcttggcg taacgctggc atcgggctac
      961 ccgagcatca tcgcaccgaa caaaaccatc accaaaaccg gtgagaagat gattatcgac
     1021 ggcctggagt ttgacttcct gatgacccca ggtagcgaag caccagccga aatgcacttc
     1081 tatattccgg ccctgaaagc gctgtgtacc gccgagaacg ccacgcatac cctgcacaac
     1141 ttctacactc tgcgcggcgc gaaaacccgc gacaccagca agtggaccga gtatctgaac
     1201 gaaacgctgg atatgtgggg taacgacgcg gaagtcctgt ttatgccgca cacctggccg
     1261 gtctggggca ataagcatat caatgattat attggtaaat atcgcgatac tatcaagtac
     1321 attcacgacc agaccctgca cctggcgaac cagggctaca ccatgaatga aatcggcgac
     1381 atgattaaac tgccgcctgc acttgccaat aactgggcca gccgtggcta ttacggttct
     1441 gtcagccaca acgcccgcgc ggtgtataac ttctatcttg gctattacga cggtaacccg
     1501 gctaacctgc atccgtatgg tcaggtggag atgggtaaac gttacgtgca ggcgctgggc
     1561 ggttctgccc gtgtcatcaa cctggcgcaa gaagcgaaca agcaaggtga ttaccgctgg
     1621 tcggcagaac tgctgaaaca ggtgattgcc gccaacccgg gtgaccaggt cgcgaagaat
     1681 ctgcaagcga ataactttga acagctgggc tatcaggccg agtccgccac ctggcgcggt
     1741 ttctacctga ccggcgcgaa agagctgcgc gaaggggtgc ataagttcag ccacggcacc
     1801 atcggttccc cggacaccat tcgcgggatg tcggtcgaaa tgctgttcga ctttatgtcc
     1861 gttcgcctcg atagcgcgaa agccgcgggt aaaaatatca gcctgaactt caatatgagc
     1921 aatggcgata acctcaacct gacgctgaac gatagcgtgc ttaactaccg taaaacactg
     1981 caatcccaag ctgacgcctc tttctacatc agccgtgaag atctgcacgc cgtgctgacc
     2041 gggcaagcca aaatggcgga tctggtaaaa gcgaagaaag ccaaaattat tggcaatggc
     2101 gcgaaactgg aagaaattat cgcctgtctg gataatttcg atttgtgggt gaatatcgta
     2161 accccaaatt aatgtcactc gct
//
LOCUS       DAEEKQ010000334         2147 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.342, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000334 DAEEKQ010000000
VERSION     DAEEKQ010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.342"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            35..367
                     /gene="hdeA"
                     /locus_tag="KLK57_004358"
     CDS             35..367
                     /gene="hdeA"
                     /locus_tag="KLK57_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HBE0894538.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            483..809
                     /gene="hdeB"
                     /locus_tag="KLK57_004359"
     CDS             483..809
                     /gene="hdeB"
                     /locus_tag="KLK57_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HBE0894539.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            873..1520
                     /locus_tag="KLK57_004360"
     CDS             873..1520
                     /locus_tag="KLK57_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HBE0894540.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNMVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1562..2092)
                     /locus_tag="KLK57_004361"
     CDS             complement(1562..2092)
                     /locus_tag="KLK57_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0894541.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
BASE COUNT          637 a          415 c          483 g          612 t
ORIGIN      
        1 acggctcttt cactttatag ttgaggatat tacgatgaaa aaagtattag gcgttattct
       61 tggtggtctg cttcttctgc cagttgtgag caatgcagcg gatgcgcaaa aagcagctga
      121 taacaaaaaa ccggtcaact cctggacctg tgaagatttc ctggctgtgg acgaatcctt
      181 ccagccaact gcagttggtt ttgctgaagc gctgaacaac aaagataaac cagaagatgc
      241 ggttttagat gttcagggta ttgcaaccgt aaccccagct atcgttcagg cttgtactca
      301 ggataaacaa gccaacttta aagataaagt taaaggcgaa tgggacaaaa ttaagaaaga
      361 tatgtaattc cgggaatgcg ttacatcgta cttccttgca tattgaacag gccggaatat
      421 cttctttaaa agcagctatt cctcctgttc atatataatc tctatattga atgggttaca
      481 aaatgaatat ttcatctctc cgtaaagcgt ttatttttat gggcgctgta gcggctttgt
      541 cactggtgaa cgcacaatct gcgctggcag ccaatgaatc cgctaaagat atgacctgcc
      601 aggaatttat tgatctgaat ccaaaagcaa tgaccccggt tgcatggtgg atgctgcatg
      661 aagaaacagt atataaaggt ggcgataccg ttactttaaa tgaaaccgat ctcactcaaa
      721 ttcctaaagt gatcgaatac tgtaagaaaa acccgcagaa aaatttgtat accttcaaaa
      781 atcaagcatc taatgacttg ccgaattaat gaggtacaag taaaaaggag tagcaagttg
      841 agccatcttg ctgctccttt ttgcattttt atatgacagc agaatttatt atacgtctta
      901 tactcgcggc aattgcctgt ggcgctattg gcatggaaag gcaaatgcgc ggcaaaggag
      961 cagggttacg cacacatgta ttaattggca tgggaagcgc cctgtttatg attgtttcga
     1021 aatatggttt tgctgacgtg ctgtctttag atcacgtcgg actcgacccc agccgtattg
     1081 ctgctcaggt ggtgacgggc gtcgggttta tcggtgcagg taacattctc gttcgtaacc
     1141 aaaatattgt aggtctgacg acggcagcgg atatctgggt gaccgccgcc ataggtatgg
     1201 ttattggcag cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc
     1261 tggaagtctt ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga
     1321 cattagtgaa cggtaatatg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa
     1381 ctgatttagt ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc
     1441 ttcatgccac aacgtcgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga
     1501 aaggcgtttc aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa
     1561 ttcacaccag ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga
     1621 ggatcatcat gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct
     1681 ccagaatatc ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc
     1741 gcgaaagtga cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa
     1801 aatcaatgat agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt
     1861 taatattaaa gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga
     1921 gaaaattaga atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat
     1981 gcaacattac gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa
     2041 tgtttttcat cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact
     2101 cctggtgcgg acattaactt tataatcaat ttgccaaagg taattaa
//
LOCUS       DAEEKQ010000335         2111 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.343, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000335 DAEEKQ010000000
VERSION     DAEEKQ010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.343"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            281..736
                     /locus_tag="KLK57_004362"
     CDS             281..736
                     /locus_tag="KLK57_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcN family protein"
                     /protein_id="HBE0894542.1"
                     /translation="MTLPVDGIKLHRGNFAAIGQQIQPLLDAGQCLRLQVKPWREKRS
                     LSQNALSHMWYTEISEYLIARGKTFATPEWVKDAMKHTYLGYESKDRVDVVSGEVTTV
                     QSLRHTSELETGEMYIFLCKVEAWAMNIGCHLTIPQSCEYQQLRDKQEA"
     gene            736..906
                     /locus_tag="KLK57_004363"
     CDS             736..906
                     /locus_tag="KLK57_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894543.1"
                     /translation="MATPLIRVMNGHIYRVSNRRKRKPELKPSEIPTLLGYTASLVDK
                     KWLRLAARRNHG"
     gene            899..1189
                     /locus_tag="KLK57_004364"
     CDS             899..1189
                     /locus_tag="KLK57_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBE0894544.1"
                     /translation="MADLRKAARGRECQVRIPGVCNGNPETSVLAHIRLAGLCGTGIK
                     PPDLIATIACSACHDEIDRRTYFVDAEYAKECALEGMARTQVIWLKEGVIKA"
     gene            1186..1548
                     /gene="rusA"
                     /locus_tag="KLK57_004365"
     CDS             1186..1548
                     /gene="rusA"
                     /locus_tag="KLK57_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RusA"
                     /protein_id="HBE0894545.1"
                     /translation="MNTYSITLPWPPSNNRYYRHNRGRTHISAEGQAYRDNVTRIIKN
                     AMLDIGLAMPVKIRIECHMPDRRRRDLDNLQKAAFDALTKAGFWLDDAQVVDYRVVKM
                     PVTKGGRLELTITEMGNE"
     gene            1545..1685
                     /locus_tag="KLK57_004366"
     CDS             1545..1685
                     /locus_tag="KLK57_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcG family protein"
                     /protein_id="HBE0894546.1"
                     /translation="MMFEFYMAELLRHRWGHLRLYRFPGSVLTDYRILKNYAKTLTGA
                     GV"
     gene            1771..>2111
                     /gene="quuD"
                     /locus_tag="KLK57_004367"
     CDS             1771..>2111
                     /gene="quuD"
                     /locus_tag="KLK57_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HBE0894547.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLITSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMAL"
BASE COUNT          544 a          488 c          559 g          520 t
ORIGIN      
        1 tttctttgtt ggtcattggt tagatccata gttctggctg tgattaggtg aaagtataca
       61 aggtttttaa agcgaagatc actgttttgg cttcaagata gttttgattt atcgtaatca
      121 atccgccata atcctctcat cggagcctga acaactccgg tgacttctgc gcatttaagg
      181 ggacttaaat gcgaccacaa tctgaactca tcaccttgtc acagatgcag aaatgcacct
      241 gcgattttct gcattctgcg gtttccgtta gggaggccgt atgactctgc cagtagacgg
      301 catcaaactc catcgcggta acttcgcggc catcggccag cagattcagc cattgctgga
      361 tgccgggcaa tgcttacgcc tgcaggttaa gccgtggcgc gagaagcgca gcctgtcgca
      421 gaacgcgctc agccacatgt ggtacacgga gatcagcgaa tatctcatcg cccgcggcaa
      481 gaccttcgct acgcctgagt gggtcaaaga cgcgatgaag cacacctatc tcggctacga
      541 aagcaaagac cgtgtagatg tcgtgtccgg cgaggtcacc accgtccaat ccctccgcca
      601 tacgtcagag ctggaaaccg gcgagatgta catcttcctg tgcaaagtcg aagcctgggc
      661 gatgaatatc ggctgccacc tgacaattcc gcagagctgt gaataccagc agctgcgcga
      721 taagcaggag gcgtaatggc tacaccgctt attcgtgtca tgaacgggca catctacaga
      781 gtatcaaatc gtcgtaagcg taagcctgag ctgaagccat ccgaaatacc aacactgctc
      841 ggatataccg ctagcctggt tgataaaaaa tggttgcgac tggcagcaag gaggaatcat
      901 ggctgatttg agaaaagcag cgcgtggtcg ggaatgccag gtaagaatcc ctggcgtatg
      961 taatggcaat cctgaaacgt cagtactggc acatatccgg ctggctggat tgtgcggtac
     1021 cggtatcaaa ccgccagacc tgattgccac cattgcatgt tctgcctgtc acgacgaaat
     1081 cgaccgccgc acatattttg tcgatgctga gtatgcaaaa gaatgcgcgc tggaaggtat
     1141 ggcgagaacg caggttatct ggctgaaaga gggggttatt aaggcgtgaa tacctacagc
     1201 atcacattac cctggcctcc gagcaataac cgctattacc gccataatcg cgggcgcaca
     1261 cacatcagcg cagaagggca ggcataccgc gataacgtca cccgaatcat taaaaacgca
     1321 atgctggata tcggcctggc tatgccagtg aaaatccgta ttgagtgcca catgccggat
     1381 cgccgtcgcc gtgacctgga taatctgcaa aaagccgctt ttgacgcact caccaaagca
     1441 ggtttctggc tggatgatgc tcaggtcgtt gattaccgcg ttgtgaagat gcctgttacc
     1501 aaaggtggga ggctggaact gaccatcacc gaaatgggga atgaatgatg tttgagtttt
     1561 atatggcaga acttcttcgc caccgctggg ggcatctgcg cttatatcgt ttccccggtt
     1621 ctgttttgac cgattaccga atactgaaga attacgccaa aaccctgaca ggagcaggag
     1681 tatgaagtca gagataacaa tcaactaata ctgttttgtt gatttttgct tgtaattggc
     1741 gttctggtct gatttttgtg gagtaagttg atgcgtgata ttcagatggt tcttgagcgt
     1801 tggggagcgt gggcggctaa taatcatgaa gatgtgacct ggtcgtccat tgccgccggt
     1861 tttaagggat taattacttc aaaagtaaaa tctcgcccgc aatgttgtga cgatgacgcg
     1921 atgattattt gcgggtgcat ggcccgtctg aaaaagaaca acagcgattt gcacgattta
     1981 ttagtagatt attatgtagt cggtatgaca ttcatgtcac tggcaggtaa gcattgctgc
     2041 tctgatggtt atatcgggaa aaggttacag aaggctgagg gcataattga agggatgtta
     2101 atggcattag a
//
LOCUS       DAEEKQ010000336         2103 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.344, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000336 DAEEKQ010000000
VERSION     DAEEKQ010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2103
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.344"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            101..1600
                     /gene="pitB"
                     /locus_tag="KLK57_004368"
     CDS             101..1600
                     /gene="pitB"
                     /locus_tag="KLK57_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HBE0894548.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1649..>2103)
                     /locus_tag="KLK57_004369"
     CDS             complement(1649..>2103)
                     /locus_tag="KLK57_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HBE0894549.1"
                     /translation="VPALVITAFVARRLLRFRHMLACRRRGLIVLTDRYPQDQIPGAY
                     DGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDLVIKLNVDLEVACARKPDHKRES
                     LARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEKAITDFMTARGYH"
BASE COUNT          486 a          518 c          557 g          542 t
ORIGIN      
        1 actcttttta tcgttaaaaa atgatatttc actttgccca tgccgttaaa gtcactgata
       61 atgcgtccgt tcgtaaattc aaaatggcgt aatctaatat atgctaaatt tatttgttgg
      121 ccttgatata tacacagggc ttttgttatt gcttgctctg gcatttgtgt tgttctacga
      181 agcaatcaat ggttttcatg acacggcgaa tgcggtggca accgttattt atactcgtgc
      241 catgcaacca caacttgctg tggtgatggc ggcatttttt aacttttttg gcgtgttatt
      301 gggcggactt agcgttgcct atgccattgt ccatatgttg ccaaccgatt tgttgctgaa
      361 tatggggtca acccacggcc tggcgatggt cttttccatg ctgctggcgg cgattatctg
      421 gaacctggga acgtggttct tcggtttacc ggcctccagt tcgcacacct tgattggtgc
      481 gattatcggc atcggtttaa ccaacgcgct gttaaccggc tcatcggtga tggatgcgtt
      541 aaacctgcgt gaagtgacca aaattttctc ctcgctgatt gtttccccta tcgtcggcct
      601 ggtcattgcg ggaggcctga tattcctgct gcgacgctac tggagcggga cgaaaaagcg
      661 tgaccgtatt caccgcattc cggaagatcg caaaaagaaa aaaggcaaac gtaaaccgcc
      721 attctggacg cgtattgcgc tgattgtttc cgctgcgggc gtggcgtttt cgcacggcgc
      781 gaacgacgga caaaaaggga tcggcctggt gatgctggta ctggtgggga ttgcccctgc
      841 tggcttcgtc gtcaatatga acgcgtccgg ctatgaaatt acccgtaccc gcgatgccgt
      901 caccaacttc gaacactacc tgcaacagca tcctgaactg ccgcagaagt tgattgcgat
      961 ggaacctcca ttgcctgcag catcgactga tggcacgcaa gtaacagagt ttcactgtca
     1021 tccggcaaat acctttgatg cgattgcgcg cgttaaaacg atgctgccag gcaatatgga
     1081 aagttacgag ccgttaagcg tgagtcagcg cagccagctg cgccgcatta tgctgtgcat
     1141 ctctgatacc tccgcgaagc tggcgaaact gccaggcgtc agtaaagaag accagaacct
     1201 gctgaaaaaa ctgcgcagcg atatgttaag caccattgag tacgctccgg tgtggatcat
     1261 catggcggta gcactggcgc tcggcattgg caccatgatt ggctggcgtc gtgtagcgat
     1321 gaccatcggt gagaagattg gtaagcgcgg catgacgtat gcgcaaggca tggcggcaca
     1381 aatgacggcg gcagtgtcta tcggccttgc cagttatatt gggatgcctg tctccacaac
     1441 acacgtcctc tcgtctgcgg tagcagggac gatggtggtg gacggcggtg ggttacagcg
     1501 taaaacggta accagcatcc tgatggcgtg ggtgtttact ttaccggcgg caatttttct
     1561 ttctggtggg ctgtactgga tagcattgca gttgatttaa ccttcctgaa aatgcccggt
     1621 cctggcgatc gggcatttcc atttttgact agtgataacc acgcgcggtc ataaaatccg
     1681 taatcgcttt ttctgcatca accaacacct gttccagtgg ctgattggca tcgatatcaa
     1741 ccagttgtgc accaccaaag gttaactgtg gcgttatggc aatcttcctc gccagcgatt
     1801 cccgtttatg gtcgggttta cgtgcacagg caacttcaag gtcaacattg agtttgatga
     1861 ccagatcagg cttatggctc gccatccagt gaaacgcttt acgttcctgg cttgccagcc
     1921 atgagacaaa acgaccacct tcaacgttag gtgggaacac cgtaccatcg taagcgccag
     1981 gaatttggtc ctgaggataa cggtcggtta gaacaattaa cccgcgacga cgacaggcaa
     2041 gcatatgacg aaagcgcagt aagcgacggg cgacaaacgc tgtaattacc agcgccggaa
     2101 ctg
//
LOCUS       DAEEKQ010000337         2101 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.345, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000337 DAEEKQ010000000
VERSION     DAEEKQ010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2101
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.345"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(60..1025)
                     /locus_tag="KLK57_004370"
     CDS             complement(60..1025)
                     /locus_tag="KLK57_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012421200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HBE0894550.1"
                     /translation="MSEDKFLSDYSPRDAVWDTQRTLTDSVGGIYQTAAEFERYALRM
                     ASCSGLLRFGWSTIMETGETRLRLRSAQFCRVRHCPVCQWRRTLMWQARFYQALPKIV
                     VDYPSSRWLFLTLTVRNCEIGELGTVLTAMNAAFKRMEKRKELSPVQGWIRATEVTRG
                     KDGSAHPHFHCLLMVQPSWFKGKNYVKHERWVELWRDCLRVNYEPNIDIRAVKTKTGE
                     VVANVAEQLQSAVAETLKYSVKPEDMANDPEWFLELTRQLHKRRFISTGGALKNVLQL
                     DRETNEDLVIADDVGDGTDDGKRTAFVWDSGKRRYKRAPEKDKSD"
     gene            complement(1891..>2101)
                     /locus_tag="KLK57_004371"
     CDS             complement(1891..>2101)
                     /locus_tag="KLK57_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894551.1"
                     /translation="RSEAVIPDRFKNLILLIDGLSFGTFGFHPHEGFEDELILYIQKT
                     NERVKTLFVKIDLNGIKFGFLRTHS"
BASE COUNT          582 a          551 c          439 g          529 t
ORIGIN      
        1 aaaaaaaata aaccaatagt cttatttcca ttcaggtatt caatattaat attcatacgt
       61 taatccgatt tatccttctc aggggcgcgt ttgtaacgcc gtttacctga atcccagaca
      121 aacgccgtcc gcttcccgtc atcagtgcca tcccctacat cgtcggcaat gacaagatcc
      181 tcattggttt ctcgatccaa ctggaggacg ttttttagcg ccccaccggt cgagataaaa
      241 cggcgcttgt gaagctgccg cgtcagctca agaaaccact caggatcgtt tgccatatct
      301 tccggtttaa cggagtattt cagcgtttca gcaaccgcgc tttgcagttg ctcggcaacg
      361 ttggccacaa cctcacctgt cttagttttt actgcccgaa tatcgatatt cggctcatag
      421 ttcacccgca agcaatcgcg ccagagttct acccaacgtt cgtgcttaac gtagttcttc
      481 cctttaaacc aagaaggttg caccatcagc agacagtgaa aatgcggatg tgcgctgcca
      541 tccttacctc gcgtcacctc cgtagccctg atccacccct gaacaggtga tagctccttt
      601 cgcttttcca ttcgcttaaa cgccgcattc attgctgtaa ggactgttcc aagttcacct
      661 atctcgcagt tcctgacagt taacgtcaga aacaaccatc gggaagacgg gtaatccaca
      721 acgattttcg gtagagcctg ataaaaacgg gcttgccaca tgagggttct tctccactgg
      781 cagacagggc aatgacggac acggcaaaat tgcgcactac gaagccgtag gcgcgtttct
      841 ccggtttcca tgatggtaga ccaaccaaaa cgtaacaaac cgctacagga ggccatacgg
      901 agtgcatagc gctcgaattc agcagcagtc tggtagatac ccccgacaga atcggtaagc
      961 gtgcgctggg tatcccaaac tgcatcacgg gggctgtagt ccgaaaggaa tttatcttct
     1021 gacattaagc ctgctccgat ctgggcaggc tgtgatttga tgtggtatat tcatcacgta
     1081 cctgtgcatt gcgagccagt acatgtcacc agagtttgcc gctgaggtga cacattcgaa
     1141 atgttgggga gtgttgacgc actccccaac gccgattaaa aacgcctcaa ttctgctcca
     1201 ttttctttga cctgtcaaaa cccactcaaa gcctcgtcat tactgggcta aaccactatt
     1261 tcatatccgc catttatccc ttaagtataa atacaaggat cacgcgcgag aattgcgcaa
     1321 aaaataacca caattttaac cccccaaaac aagcaccccc gaacggctta ccccaacctt
     1381 ttccacacac ccgatcacca aaacagggaa taaaaacaaa accctgtctt acaggtggat
     1441 ggcctcactt cgtgaaacca tccaccccgc ataagttttt taatcggtgg tgtcgtcaat
     1501 cactaaatcg gaaccccaaa ggggaccccc acgatttagc atttgacgag attaactctc
     1561 ggagatggca tcatgctttt aacggacttt aaaaaacggt gaacggatgc ggattttaat
     1621 ccgtgtctgt gaggcgttga gcaacgagca aagcgaggcg gtagaaagag agccagcggc
     1681 aggctcagga cgataatccg gggaaaatat accactcgca gagtgttaac atttgacatc
     1741 ggattgtcta tcctgcattg gtgtcgagtt ggcaaggctg aggataatgc tgaggataat
     1801 gctgaggata atgcaggata gacagagcac tagagattgc atgcaatctc gtacctatca
     1861 cctcagcctg ccgctggctc ttttccagaa ctaagaatgc gttcttaaaa atccaaactt
     1921 gataccgttt aaatctattt tcacaaaaag agtttttacc ctctcgtttg ttttctgaat
     1981 atataaaatt aattcatcct caaaaccttc gtgcggatga aaaccaaatg tgccaaaact
     2041 taatccatca ataagcaaaa taaggttttt aaacctgtca ggaatcaccg cttcacttct
     2101 t
//
LOCUS       DAEEKQ010000338         2094 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.346, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000338 DAEEKQ010000000
VERSION     DAEEKQ010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2094)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2094)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2094
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.346"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(36..974)
                     /gene="mdh"
                     /locus_tag="KLK57_004372"
     CDS             complement(36..974)
                     /gene="mdh"
                     /locus_tag="KLK57_004372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HBE0894552.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGEEFVNK"
     gene            1409..1879
                     /gene="argR"
                     /locus_tag="KLK57_004373"
     CDS             1409..1879
                     /gene="argR"
                     /locus_tag="KLK57_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HBE0894553.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT          570 a          528 c          444 g          552 t
ORIGIN      
        1 gtctgtgctc cggtttttta ttattcgcta atcaattact tattaacgaa ctcttcgccc
       61 aaggcgatat ctttcttgag tgtatccagc ataccttcca gcgcgttctg ttcaaatgcg
      121 ctcagggtac cgatagattt acgctcttcc acgccgtttt tacccagcag cagcggttga
      181 gagaagaaac gggcgtactg accatcgcct tcaacgtagg cacattcgac aacgccttgt
      241 tcgccctgca gtgcacgaac cagagacaga ccaaaacgtg cagctgcctg gcccatagac
      301 agggttgcag acccgccacc ggctttcgct tcaaccactt cagtacccgc gttctggatg
      361 cgtttggtca gatcagccac ttcctgctcg gtaaaactaa cgccaggaac ctgtgacagc
      421 agcggcagaa tggtaacacc agagtgaccg ccaataaccg gcacttcaac ttcgcctggc
      481 tgtttgcctt tcagttccgc aacaaaagtg ttggaacgaa tgatgtccag cgtggtaacg
      541 ccgaacagtt tgtttttgtc ataaacaccg gcttttttca gcacttcagc agcaatcgca
      601 actgtggtgt taaccgggtt agtgataata ccaatgcacg ctttcgggca ggttttcgca
      661 acttgctgta ccaggttttt cacgatgccg gcgttaacgt taaacaggtc ggaacgatcc
      721 atacccggtt tacgcgctac acctgcagag ataagaacga catctgcgcc ttccagcgcc
      781 ggagtcgcat cttcaccaga aaaacctttg attttcacag cagtagggat atggctcaga
      841 tcgacagcca caccgggagt cactggagcg atatcataca gagagagttc tgaacctgaa
      901 ggcagttggg tttttaacag tagtgcaagc gcctggccaa taccgccagc agcgccgagg
      961 actgcgactt tcatcctaaa ctccttatta tattgataaa ctaagatatg ttgctccgct
     1021 gccgcgacct taatccacaa aattgccgtt tacaatgacc acatctcaag aatgtgtagt
     1081 cacgcaagtt tagcgtttat gcatttaatt gccgtaatca ggaacctaac ttacgtaatt
     1141 aacagtcgct tatgcattag cgcaacattc caacaggtgg tgacaatata ccctaccgtt
     1201 cagccaaaac aacatcaatt tgataacaat taatttactt ttaaacagaa tttgcatgcc
     1261 gtgacgcagg catgtttctc aataacgaaa tttgataaaa tcccgctctt tcataacatt
     1321 atttcagcct tcttcagggc tgactgtttg cataaaaatt catctgtatg cacaataatg
     1381 ttgtatcaac caccatatcg ggtgacttat gcgaagctcg gctaagcaag aagaactagt
     1441 taaagcattt aaagcattac ttaaagaaga gaaatttagc tcccagggcg aaatcgtcgc
     1501 cgcgttgcag gagcaaggct ttgacaatat taatcagtct aaagtctcgc ggatgttgac
     1561 caagtttggt gctgtacgta cacgcaatgc caaaatggaa atggtttact gcctgccagc
     1621 tgaactgggt gtaccaacca cctccagtcc attgaagaat ctggtgctgg atatcgacta
     1681 caacgatgca gttgtcgtga ttcataccag ccctggcgcg gcgcagttaa ttgctcgcct
     1741 gctggactca ctgggcaaag cagaaggtat tctgggcacc atcgctggcg atgacaccat
     1801 ctttaccacc cctgctaacg gtttcaccgt caaagacctg tacgaagcga ttttagagct
     1861 gttcgaccag gagctttaat ctctgccccg tcgcttctgg cggcggggaa aatgttgctt
     1921 attcctctca accccctgct ttcccctgcg attaatttaa cgaatagtgc gttttaccgc
     1981 gacatatcat tcacacaatg aatacatacg gtaaaaaaag cacattatgt aaaattcatt
     2041 atctaattga aaatactaga attaacaata aataaccgtg tttttaattc tttt
//
LOCUS       DAEEKQ010000339         2059 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.347, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000339 DAEEKQ010000000
VERSION     DAEEKQ010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.347"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            142..1275
                     /gene="ompN"
                     /locus_tag="KLK57_004374"
     CDS             142..1275
                     /gene="ompN"
                     /locus_tag="KLK57_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HBE0894554.1"
                     /translation="MKSKVLALLIPALLAAGAAHAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNSAKDGDQSYARLGFKGETQINDQLTGYGQWEYNIQANNTESSKNQSWTRLAFAGL
                     KFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGDSYTNADNFMTGRANGVATYRNTDFF
                     GLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVRHENGDGWGLSTTYDLGMGFSAGAAY
                     TSSDRTNDQVNHTAAGGDKADAWTAGLKYDANNIYLATMYSETRNMTPFGDSDYAVAN
                     KTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHAADGADNPAGVDDKDLVKYADVGATY
                     YFNKNMSTYVDYKINLLDEDDSFYAANGISTDDIVALGLVYQF"
     gene            1416..1850
                     /gene="uspF"
                     /locus_tag="KLK57_004375"
     CDS             1416..1850
                     /gene="uspF"
                     /locus_tag="KLK57_004375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HBE0894555.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
BASE COUNT          568 a          465 c          462 g          564 t
ORIGIN      
        1 tttcagaaga attatttgat taaggtttta cttaaggcgt aacaaatgat atttatcggc
       61 taactgaact tctcctttag gatgttttca ctccccggaa tggggataaa aggcaaataa
      121 aataactaaa ggatttattc aatgaaaagc aaagtactgg cacttttaat tcctgccctg
      181 ctcgccgcag gtgctgcaca tgcagccgaa gtttataata aagacggcaa caaattagat
      241 ctgtatggca aagttgatgg cctgcattat ttttctgata attcagcgaa agatggcgat
      301 cagagctatg cgcgtctggg ttttaaaggc gaaacccaaa ttaacgatca actcactggc
      361 tacggtcaat gggaatacaa tattcaggca aacaacactg aatcttcaaa aaaccagtca
      421 tggacccgtc tggcatttgc cgggctgaaa tttgcagatt acggttcttt cgattacgga
      481 cgtaattatg gcgtaatgta cgacatcgaa ggctggaccg atatgctgcc tgaatttggc
      541 ggtgactctt ataccaatgc agacaacttt atgactggtc gtgccaacgg cgtcgcgact
      601 tatcgtaata ctgatttctt cggtctggta aatggtctga acttcgcggt gcagtatcaa
      661 ggtaacaacg aaggtgccag taatggtcag gaaggcacca acaacggacg tgatgttcgc
      721 catgaaaatg gtgacggctg gggtctttcc acaacatatg atttaggcat gggctttagc
      781 gctggtgcgg catacacctc atctgaccgc accaatgacc aggttaacca tactgcggcg
      841 ggtggtgata aagcagacgc gtggactgct gggctaaaat acgatgctaa caatatttac
      901 ctggcaacca tgtattcaga aacgcgtaat atgaccccgt ttggcgacag cgattatgct
      961 gttgcaaaca aaacccagaa ctttgaagtc actgcacagt accagtttga ttttggtctg
     1021 cgtccggcag tctctttcct gatgtctaaa ggccgtgacc tgcacgctgc cgatggtgca
     1081 gacaacccgg caggtgttga tgataaagat cttgttaaat acgccgatgt tggcgcgact
     1141 tactatttca ataaaaacat gtccacttat gttgactata aaatcaacct gttggatgaa
     1201 gatgacagtt tctacgctgc caacggcatc tctaccgatg atattgtcgc tttaggtctg
     1261 gtttatcagt tctaaatcct cctgcccgct gttatggcgg gctttttctg cttattcttc
     1321 ctcctttgat ctaaattaaa aatgtgaact ccgtcattac acaaaaagtg tcatctggcg
     1381 ttacacttta tgcggatact aaaacaggag gttttatgaa cagaacgatt cttgtcccta
     1441 tcgatatttc cgattcagaa ttaactcaac gcgtgattag ccacgttgag gcagaggcaa
     1501 agattgacga tgcagaggtt catttcctga cggtaatacc ttcactgcct tactatgcct
     1561 ctctgggttt agcgtattcc gcagaattac cggcaatgga tgacctgaaa gcggaagcca
     1621 aatcgcaact ggaagagatc attaaaaaat ttaaactgcc aaccgacaga gtgcatgtcc
     1681 atgttgagga aggctcgccc aaagaccgca ttctggaatt ggcgaagaag atccccgctc
     1741 atatgatcat cattgcttcc catcgaccgg atatcaccac ttatctgctc ggttccaacg
     1801 ccgcagctgt agtgcgtcac gcagagtgct ccgtgctggt tgtgcgctga cactaacgcc
     1861 cgcacattgc tgcgggcttt ttgattcatt tcgcaaatgt gctgacattt tcccctctaa
     1921 tccgtaccat acacgccaca gttttatatc agatttctta tgctgggcgt tccggcatga
     1981 cgcatactct tctgatgcca tataacgaat tgagtcgctt ttaaatgtcg caaaatcaag
     2041 aaattagtaa gaaagaaca
//
LOCUS       DAEEKQ010000340         2044 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.348, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000340 DAEEKQ010000000
VERSION     DAEEKQ010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2044)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2044)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2044
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.348"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            120..656
                     /locus_tag="KLK57_004376"
     CDS             120..656
                     /locus_tag="KLK57_004376"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HBE0894556.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            771..1097
                     /locus_tag="KLK57_004377"
     CDS             771..1097
                     /locus_tag="KLK57_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HBE0894557.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(1285..1524)
                     /locus_tag="KLK57_004378"
     CDS             complement(1285..1524)
                     /locus_tag="KLK57_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HBE0894558.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT          591 a          443 c          435 g          575 t
ORIGIN      
        1 gcattaaatt aaaactctac cggtaaacaa gcatctctag gcgtaaaaaa caggcacttt
       61 tttaaatccc agctagaata ggctgtatag gcagacagtt gcaagacaag gagcgtttta
      121 tgtctaacag tccattttta aattctatac gcacggatat gcgacaaaaa ggttatgcgc
      181 tgaaaactga aaaaacttac ctgcactgga ttaagcgttt tattctgttt cacaaaaaac
      241 gtcatcctca gaccatgggc agtgaagagg tcaggctgtt tttatccagc ttagcaaaca
      301 gcagacatgt agccataaac acgcagaaaa tcgctttaaa tgccctagct tttttgtaca
      361 acaggttttt acaacagccg ttgggcgata ttgattatat ccctgcaagc aagcctagac
      421 ggctaccctc tgttatctct gcaaatgaag tgcaacgcat tttgcaggtt atggatactc
      481 gcaaccaagt tatttttacg ctgctgtatg gtgcaggttt gcgcattaat gaatgcttgc
      541 gtttgcgggt taaagatttt gattttgata atggctgcat cactgtgcat gacggtaagg
      601 gtgggaaaag cagaaacagc ctactgccca cgcgcctaat cccagcaata aaataactca
      661 ttgagcaagc gcggcttatt cagcaagacg acaacttaca aggcgtaggg ccatcgctgc
      721 cttttgcttt agatcacaaa tacccttctg cttatcgaca agcggcgtgg atgtttgtct
      781 ttccctccag cacgctctgc aaccacccgt ataacggcaa attatgccgc catcatctgc
      841 atgactccgt tgcgcgaaag gcattgaagg cagccgtaca aaaagcaggc atcgttagca
      901 agcgtgtcac ttgtcataca tttcgtcact cgtttgctac gcatctatta caagcggggc
      961 gtgatattcg cactgtgcaa gaactcttag ggcataacga tgttaagacc acgcaaatct
     1021 atacgcatgt gttgggtcag cattttgccg gcaccaccag tcctgcggat ggactgatgc
     1081 tacttatcaa tcagtaaaag tagataaata tctttatgta gccctgagta gcccctgata
     1141 ctttttatgg tgaaaaagat aaacaatcat agaggggata aatatgagcc agttctacca
     1201 ttgttcagat aagtttaagc aagatactgt caatcacagt gaccattcat ggtcattaca
     1261 cttcctccag ttgccagaca atgcctaaca taaaaacgca aaagcccaaa agtcagagct
     1321 taggtgttct aggcgttttc taagcgctga agaaatacgt cttaccgtag attgcgtgcg
     1381 tttctgaagc acattttcct tatacatggc tttatcccag gcagcttcat caagctcttg
     1441 gagtaacaag ccagtaataa tgcgacactc tcgggtttgt agcgagccgc tggtgagatc
     1501 ggagttatag cggaaagggg tcatttgtaa ttatttgtgg gtggaggata gttaacttat
     1561 tattgtagtt ggaagaggct gctttccaaa cattagcacc tcgtaatgct tgttactgta
     1621 tattaattta tgctcttagc gcatgttaat gggctattac aatgcataat gtccacatca
     1681 agataactgt ggcttaacac ctgtacaagc gaaaaaaatc aatcacctgt ccggaaatac
     1741 gggatcactg taaagtgttg gtgaggcagt ctaggagggg gatttaattt gggatactga
     1801 tgaaaggatt atgatcagtt ggatatagga tcgtactaag ctgagcggca ggttagctag
     1861 attagttaac cagataagtg aaatctagtt ccaaactatt ttgtcatttt taattttcgt
     1921 attagcttac gacgctacac ccagttccca tctattttgt cactcttccc taaataatcc
     1981 ttaaaaactc catttccacc cctcccagtt cccaactatt ttgtccgccc acaagggctg
     2041 ccga
//
LOCUS       DAEEKQ010000341         2040 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.349, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000341 DAEEKQ010000000
VERSION     DAEEKQ010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.349"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(77..412)
                     /locus_tag="KLK57_004379"
     CDS             complement(77..412)
                     /locus_tag="KLK57_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin transporter"
                     /protein_id="HBE0894559.1"
                     /translation="MNRKYYFNNMWWGWVTGGYMLYMSWDYDFKYRLLFWCISLCGMV
                     LYPVAKWYIEDTALKFTRPDFWNSVFFADTPGKMGLLAVYTGTVFILSLPLSMIYILS
                     VIIKRLSVR"
     gene            541..888
                     /locus_tag="KLK57_004380"
     CDS             541..888
                     /locus_tag="KLK57_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894560.1"
                     /translation="MTFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVTVLTGGLWGISWGCAMWFIYWGPSGMVAGEAIIISITGGFLSGLLMASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(908..1498)
                     /locus_tag="KLK57_004381"
     CDS             complement(908..1498)
                     /locus_tag="KLK57_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894561.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLEQSGEA
                     PVYGPNLRASCRRLEDAGWLRTLHAPNLQLAVELTDAGRAVAQPLLLAEQDRIRAEQR
                     AAEVVVLPLVPAAGLPAGGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     WVVRREGDPLEVAQWLPACHDAGMEVRVQINESAAP"
     gene            complement(1495..1755)
                     /locus_tag="KLK57_004382"
     CDS             complement(1495..1755)
                     /locus_tag="KLK57_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001523449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894562.1"
                     /translation="MDQEMTFSLSYEQLTRFAEKRIRECNLDSHGVTYLCESAKAGAV
                     LIFWHELAINGYTSMNAIKRQEIIDADHQRLRKLIWPEDDWK"
     gene            1853..>2040
                     /locus_tag="KLK57_004383"
     CDS             1853..>2040
                     /locus_tag="KLK57_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0894563.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWATICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
BASE COUNT          484 a          542 c          511 g          503 t
ORIGIN      
        1 gagtagaacg gcaactttta gataattctt gtggcataag ttctggggta tttaaataaa
       61 atataagaca ggctgtctat cttacagaca gcctttttat aataacagaa agaatatata
      121 tcatacttaa cggaagagat aatatgaaaa cagtacccgt ataaacagca agcaatccca
      181 tttttccagg tgtatcagca aaaaaaacgc tgttccagaa atcaggccgg gtaaatttca
      241 gagctgtatc ttcaatatac cattttgcaa ccggatacaa aaccattccg cagagagaaa
      301 tacaccagaa cagtaatctg tatttaaaat cataatccca tgacatatac agcatatatc
      361 ctcccgtcac ccatccccac cacatattat taaaataata ttttctgttc atctgccagt
      421 tttcctttct tttgcacttt tgtcagtgta ctgatgcatg acaacataac cacagcatga
      481 taataataat caataacaat aagctgtgtc acgtttacat catggaacgg aggtgctcct
      541 gtgacttttg agcagaaaaa agcccgggcc attgccctga tggacagcaa aaagatgtgg
      601 cggagcaatt atgcccctcc tcttctgcgt attctctggc ggctgggtat ccggcttccg
      661 ccattaccct ttatgccgtt ctggcaggtc acggtgctga cggggggctt atggggcatt
      721 tcctggggat gtgcaatgtg gtttatctac tgggggccgt caggaatggt ggcaggtgag
      781 gccattatta taagtatcac ggggggtttt ttgtccggac tgctcatggc ctctttccac
      841 tggtggcgca ggaaagttaa ccgactgcca ccatgggatg atgtgtaaac cgtgcacagg
      901 cgtgttctta cggagcggca ctttcattta tctgtacgcg aacctccatt cctgcatcgt
      961 gacaggccgg cagccactgc gccacttcca gcggatcgcc ctcccggcgt accacccagc
     1021 cttctttatt ccataactgc agacaggtgc tgccgtcgag acgcaccaca aaatccccac
     1081 ggcaggcctg atacgttatg ccgttcagct ccaccgccag atcagtggca gaagtgccgc
     1141 ctgcgggcag acctgctgcc gggaccagcg gcagtacgac gacttctgcc gcacgctgtt
     1201 ctgcccggat gcggtcctgt tctgccagca gcagaggctg tgccacggcc cggccggcgt
     1261 ccgtcagctc cactgccagc tgcaggttcg gagcgtgcag ggtacgcagc cagccggcat
     1321 cctccagacg acggcaggag gcgcgcaggt ttggcccgta gaccggggct tccccgcttt
     1381 gctccagcac ccgttctatg tcccgcgtca gcacgggtcc tggccgtttt tcatcaaggg
     1441 cagccagcac aatcagcact ctccgctgca gtggcgacgg tcggcggctc tgagtcattt
     1501 ccagtcatcc tccggccaga tgagctttct gaggcgctga tggtcggcgt caatgatctc
     1561 ctgtctcttt atggcattca ttgaggtata gccatttatg gccagttcat gccagaagat
     1621 cagcacggct ccggctttgg ctgactcgca gagataagtt acgccgtggc tgtccagatt
     1681 gcattcccgg atgcgttttt ccgcaaaccg ggtcagttgt tcatagctga gtgaaaacgt
     1741 catttcctga tccatcctgc acgttccatc tgataatgtt ctgacaggta aaaatcattg
     1801 aaccgccccg ggaatcctgg agactaaact tcctgagaaa gaggtaaaca ggatgactaa
     1861 aaatactcgt ttttcccccg aagtccgtca acgggcagtc cgtatggttc tggaaagtca
     1921 gggcgaatat gactcacaat gggcgacaat ttgttccatt gctccaaaga ttggctgtac
     1981 gccggagact ctgcgtgtcc gggttcgcca gcatgagcgg gataccgggg gcggtgatgg
//
LOCUS       DAEEKQ010000342         2035 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.350, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000342 DAEEKQ010000000
VERSION     DAEEKQ010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.350"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            247..1116
                     /locus_tag="KLK57_004384"
     CDS             247..1116
                     /locus_tag="KLK57_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HBE0894564.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWGQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSVWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(1276..1869)
                     /gene="rclC"
                     /locus_tag="KLK57_004385"
     CDS             complement(1276..1869)
                     /gene="rclC"
                     /locus_tag="KLK57_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HBE0894565.1"
                     /translation="MEKYLHLLSRGDKIGLSLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQSANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAYTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(1881..>2035)
                     /locus_tag="KLK57_004386"
     CDS             complement(1881..>2035)
                     /locus_tag="KLK57_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HBE0894566.1"
                     /translation="KHISVSSVSASPTVLEDTIADIARKYNASSWKVTSMRIDNNSTA
                     TAVLYK"
BASE COUNT          585 a          412 c          454 g          584 t
ORIGIN      
        1 cattctccat taattcttcg tgatatcgca cattttcgtt atgcccaagg ccctaagtgt
       61 atcaaaaaca gcaaggtcga aggcaaaagc actggtagcg tctgcttcgt tgtccccgaa
      121 aatagagagg tttcagtcct acattattaa tgaatttttt gcataagtga tatcaaaatc
      181 cacatactaa tttgaggtta tgttttaacg tagactcatt gttcatgcct aatggaggga
      241 ctgacagtgg aattttccgt attaagtaac aatctgaaaa tgccgatgat gggatttggt
      301 gtttttcagg ttaccgataa aaacgtgtgc aaacagtcag tgttgaacgc tatccgcacg
      361 ggttatcgac tcatcgacac tgcagcggta tacggtaatg aagatgccgt cggtgaagcc
      421 gttcgtgaag ctatatctga aggtttatgt acccgagatg agttatttat aacgtctaaa
      481 ttgtgggggc aggatatgtt gaatcaggat acggcagcag caggtattga agcatcatta
      541 aaaaaatcgg gactagagta cttcgacctc tatttattgc accaggctat gcgcgattat
      601 ttcagtgtgt ggcgtgcact cgaagatgcc tatgaagagg gcaaattaaa agcaattggg
      661 gtttccaatt tctatcctca tgttctggcg aacttttgtg aaacggtaag agttaaaccg
      721 atggtcaacc aggtcgagtt gcatccctat tttgcccaac cagaggcgct ggcaaccatg
      781 aagtattata acgtgcagcc tgaagcatgg gctccgttag gtggtggacg acataaaccc
      841 tttgaaaata atctgcttca gagtattgca gatgcccatc aaaaatcgat ttctcaagtc
      901 attctgcgtt ggaatattca acggggagtg gtcgttattc cgaaatcgac acatcaacag
      961 cgtatcgaag aaaattttgc tatctgggat ttctcactga cagagaaaga aatggcacaa
     1021 attagttcgc ttgatttggg ttatgttggg gagtcggtaa aacattttaa tcctgaattt
     1081 gttcgtggtt gtcttgctgt aaaaatacat gattgatatt aatcatatat tttacctgag
     1141 acgacaagaa tcttttaaca ggggagtgat attgatcttc actctgtcat atctccggta
     1201 atatggcgtc aggcttcatc atcagaaagg gggcctgacg cctgaaaaag tgaacaacag
     1261 acagtgttcg gattatcaat attcagtttt taacgttgaa ctggattcat tactgcgttg
     1321 tttaagaatt tcccgcgccg aatctgccat tatcatgaca gcacctgcca gcatcagagt
     1381 atctttcaat accaagcgac cagcaccgga taaataaggg aaaccatgat gagcgtcacc
     1441 caatgcgggt acccatgcct ccggggtggt gattaaaaat gagagtgtta ccagcggcgt
     1501 ggtatatgcc atcagtcctc ccaataaacc taaccagcga ttgacaggat tagccaaaac
     1561 cagcagagca ataatcacct ccacgacgcc aagaccgttg gaaaaaccat aggtattatt
     1621 ggccgattgc catgcccttg cttctggttt gtattcgcct tcgtgagtca gatactgttt
     1681 atagtcttcc gggtgttcat aaaagaacga cattagtgga ctgtttgcga cgaatggtgt
     1741 aatgctgtct gcctcgtaag ggacaaactt taataaccca atccacataa aaacaatcgc
     1801 aatactcaga cgaatcaatg acaggccaat tttatctccc cgacttaaca ggtgcaggta
     1861 tttttccatt gtgaacatcc ttatttatac aatactgctg ttgcggttga attattatca
     1921 attcgcatcg atgtgacttt ccaggatgaa gcattatatt ttctggctat atcagcaatg
     1981 gtatcctcca gaactgtcgg tgatgctgat actgacgaga cgctgatatg tttca
//
LOCUS       DAEEKQ010000343         2032 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.351, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000343 DAEEKQ010000000
VERSION     DAEEKQ010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.351"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            9..756
                     /gene="traX"
                     /locus_tag="KLK57_004387"
                     /pseudo
     CDS             9..756
                     /gene="traX"
                     /locus_tag="KLK57_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
     gene            811..1371
                     /locus_tag="KLK57_004388"
     CDS             811..1371
                     /locus_tag="KLK57_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HBE0894567.1"
                     /translation="MTEQKRPVLTLKRKTEGETPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAAARLDKIRRQNRIKAELQAVLDEK"
     gene            1501..1713
                     /locus_tag="KLK57_004389"
     CDS             1501..1713
                     /locus_tag="KLK57_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HBE0894568.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
BASE COUNT          499 a          474 c          613 g          446 t
ORIGIN      
        1 aaacactgat gacaacggat aacacgaaca cgacacgtaa cgattcactg gctgcacgga
       61 ccgatacctg gttgcagtca tttctggtct ggtcacccgg acagcgggat atcatcaaaa
      121 cggtggcact ggtgctgatg gtactggacc acatcaatct gatattccag ctgaagcagg
      181 aatggatgtt tctggccggg cgaggagcct ttccgctttt tgccctggtg tgggggctga
      241 atctgtcccg tcatgcgcat atccggcaac cggccattaa ccggctgtgg ggatggggaa
      301 ttattgccca gttcgcatat tatctggcag gctttccctg gtatgagggg aatatcctgt
      361 ttgcctttgc agtggcggca caggtgctga cgtggtgtga aacgcgcagt gggtggcgta
      421 cagcagcggc cattcttctg atggccctgt tgggggcctt tgtccggcac cagttacggc
      481 attgccgggc tgctgatgct ggcagtcagc caccgactgt accgggcgga agacagagcg
      541 gaacgtctgg cgctggtcgc ctgcctgctg gctgttattc cggcgcttaa ccttgccacc
      601 agtgatgcag cggcggtggc cggtcttgtg atgacggtgc tgaccgttgg tctggtgtcg
      661 tgtgcaggaa aatcattacc ccgtttctgg cccggggatt ttttcccggt attctacgcc
      721 tgtcatctgg ctgtgctggg cgttctggcg ctgtgacggg tgtggtatct ttgaccgtaa
      781 caggatgatt cgtcagaggc agaacacagc atgacagagc agaagcgacc ggtactgaca
      841 ctgaagcgga aaacggaagg ggagacgcct gtccgcagcc ggaaaaccat catcaatgtc
      901 accacgccac caaagtggaa ggtgaaaaag cagaagctgg ccgagaaagc agcccgggaa
      961 gcagagctgg cggcaaaaaa agcgcaggcc agacaggcac tgtccattta tctgaacctg
     1021 cccacgctgg atgaggccgt gaataccctg aaaccctggt ggccgggatt atttgacggt
     1081 gacacccccc ggcttctggc ctgcggtatc cgggacgtgt tactggaaga cgtggcgcag
     1141 cggaatatcc cgctctcgca taaaaaactg cgcagggcgc tgaaggccat cacccgttca
     1201 gaaagctatc tgtgtgccat gaaagccggt gcctgccggt atgacacgga agggtatgtg
     1261 acggagcata tttctcagga ggaagaagcg tatgcggcag cgcgtctgga taaaatccgc
     1321 cgccagaacc ggataaaggc agaacttcag gccgtgcttg atgagaaata aaaaaaagcc
     1381 tccatcagag aggaggcagg gaaataaggc ttaaaaggaa ataacttccg cttccgaaag
     1441 gaagccagtc cactgaatta tttatcacaa taatactgac aactaaaggg ggacaagaaa
     1501 atgaatggat tcagaaacag ttcacggaac ggccaggtct ggcgttacca gcgtgccggc
     1561 gggcgggccg ttattctgga agtcagtgga cgctggatgg aagcggcaga agcatggcga
     1621 cgggctgcct gtgttgcgcc caggacagac tggcagcagt ttgcccgaaa aagggctgaa
     1681 cactgccacc ggcgctgccg gggaagggtg taacgaaaaa agcagccttc attactccag
     1741 gagtcagaag gctgaaagga gtttatgaga tttgcttttt ttaatatccg gtcgggcagt
     1801 atgaccggac agttctgagt agcacagagt tttttccgta ttaagtcgtg attttatatt
     1861 ttgtgattaa tttcacaaaa taaggtgtta ttcagtgtgt gctgcaatat tcaggatgcc
     1921 tgaaatacag atgctattta tctgatatgg agaataacat gaggaaatag taaggcagta
     1981 tgactttccg tgagacagat gctggccaac gtgggatatt taacgcgggt ta
//
LOCUS       DAEEKQ010000344         2016 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.352, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000344 DAEEKQ010000000
VERSION     DAEEKQ010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.352"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..102)
                     /gene="prlF"
                     /locus_tag="KLK57_004390"
     CDS             complement(<1..102)
                     /gene="prlF"
                     /locus_tag="KLK57_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HBE0894569.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALK"
     gene            complement(251..1822)
                     /gene="garD"
                     /locus_tag="KLK57_004391"
     CDS             complement(251..1822)
                     /gene="garD"
                     /locus_tag="KLK57_004391"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HBE0894570.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHTPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGVQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEESIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
BASE COUNT          469 a          538 c          494 g          515 t
ORIGIN      
        1 ttttaaggcc tcacgcactg gcgcggggat agttgtttgt ccgcgtatcg tgacctttga
       61 ttcagtggtc agtacagcgt gagagcgagc attagctggc atgattcatt gtccttttac
      121 agcctgtttt ctgctcaaat tataagcttg aactaaggta atgcaaatgc attattaatg
      181 gacgtggggg ctttaaacaa taaaaacatg gcactaaggc catgttttgc gagggacgtt
      241 ccaaaagaaa tcaggtcacc ggtgccgggt taaacaccgc cagctggtta tgcagccccc
      301 attgatccga gaaggttttc ttcttcccgc tggcgacgtc gagaataaag tggaacaact
      361 tccagcccac ctcttcaatg ctctcttcgc cggtagcgat agtgcccgcg ttaatatcca
      421 ttaaatcaaa ccagcggttc gccagctcgg tgcgggttgc cattttaatt acgggtaccg
      481 ccatcaggcc gtacggcgta ccacggccgg tcgtaaacac ttgtacggtg atacccgaag
      541 ccacctgttg cgtgccacag acaaaatcgc tggctggcgt cgcggcgtaa attaatccgc
      601 gtttagtcgg gcgttgaccg ggcgacagca cttcaacaat tgcactttta cccgatttag
      661 caatggagcc gagtgccttc tccaccacat ttgccagacc gcctttcttg ttgcccggcg
      721 aagggttggc gctgcggtcg gtttttccca tattgagata gttatcgtac cactccatct
      781 cctccagcag ccgtttgccg acctcttcgt tcactgcgcg tggtgtcagc agatggatcg
      841 cgtcacgcac ttccgttact tctgaaaaca tcaccgttgc gccgcagcgc accagtagat
      901 cagacgcata gccaaccgcc gggtttgccg ttacaccaga aaacgcatcg ctgccaccgc
      961 actgcacacc aacgaccagt tctgaagccg ggcaggtttc tcgctgccgt tgattcagtt
     1021 tttgtagatg gcgttcggcg acctgcaaaa tatcctcgac catggactga aagccgacat
     1081 gcttttcatc ctgcaaactg acaatgctgg cgctttctac tggaatagct tgcacatcat
     1141 ccgtgccgac cagcaggcgc tcaggctgca acttttcaca acccagacca atcaccatta
     1201 cttcgccgcc aaagttaggg ttcagcgaaa tattgtggat ggtacgaata ggcacaactg
     1261 ccgccggtgc gttaatcgcc acgccacagc cgtacaaatg attcagcccc accacgccat
     1321 cgacgttcgg gtatttcggt agcagatcgc gttcaatgat ttttactaca tagtccacca
     1381 cgcctgccac acagtggacg ctggtggtga tgccgagcag gtttttggtg cccacgctgc
     1441 catcggcatt gcgatagccc tcaaaggtgt atccttccag cggcggtaag ggggccggga
     1501 ctttggttgc cagtggcagc gtgtgtaacg gcggcgcttc cggtagtaca accattgatt
     1561 cgtcgatcca gcttccgcgt gggattgcgc gcacggcgta accaatcact tcgccataac
     1621 gaataatttc accattagct ggaatgtcca gcaatgcgac tttatgcccc tggggagtat
     1681 gttcaattaa ttccagccca tccggaaaac gcgttcctgc tttcaggcca ttatcattaa
     1741 caataattgc cacattatct gtgtcgtgaa cttttatata aaacgcagtt ggcgtttctt
     1801 gtctgatttc gatgttggcc attgtgcaat attctccagc caggggttat taaaatgagg
     1861 atctggtctc atttaaaaat aaatgacttc attgggtgaa ataagaatgt caggagttta
     1921 aacgcagaaa tgtgatcaat gtcaatcatt tcagggattg cagtgccgcc cggtaaggta
     1981 aagcgcaaat ctgtgcttta gcgcgcaacc ttatgt
//
LOCUS       DAEEKQ010000345         2013 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.353, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000345 DAEEKQ010000000
VERSION     DAEEKQ010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.353"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(37..273)
                     /locus_tag="KLK57_004392"
     CDS             complement(37..273)
                     /locus_tag="KLK57_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HBE0894571.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            414..1202
                     /gene="fhuF"
                     /locus_tag="KLK57_004393"
     CDS             414..1202
                     /gene="fhuF"
                     /locus_tag="KLK57_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HBE0894572.1"
                     /translation="MAYRSAPLYEDIIWRTHLQPQDAGLAQAVRATIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQLYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="KLK57_004394"
     CDS             complement(1240..1917)
                     /gene="bglJ"
                     /locus_tag="KLK57_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HBE0894573.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(1875..>2013)
                     /locus_tag="KLK57_004395"
     CDS             complement(1875..>2013)
                     /locus_tag="KLK57_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HBE0894574.1"
                     /translation="AIHSNAEMYAWINSAQGARELNLPSVYGDAAEWNTAELRREMSH
                     S"
BASE COUNT          527 a          530 c          439 g          517 t
ORIGIN      
        1 tatctttact tcctttcttg tttcttcctt gatttttcat ccctgattca aattcattat
       61 catcagcatg atggcagcaa taagcgccag gcacgatggt agcaaaacgt aatgccgtaa
      121 ctgtttgtga tacagcatca ccggcgtcaa tactaatccc atcagaatga caatccgcca
      181 ctgatccggc gataaatccg catacatcag ccctgcaatc agcaatcccg gcagcaacac
      241 tccccagcca ctgcccagcg tacgttgcaa catggtttca tctctttcat tgataatgat
      301 aaccaatatc atatgataat ttttatcatt tgcaagccag agaaatccct tgctatcggg
      361 taaacctatc gctaggatta gcaatcatta tcatttagat tactatcccg attatggcct
      421 atcgttccgc accgctctat gaagatatca tctggcgaac gcatctccag ccgcaggatg
      481 ctgggcttgc acaagccgta cgtgcgacga tcgccaaaca tcgtgaacat ttgctcgagt
      541 ttatccgcct ggatgaaccc gctccgctta acgccatgac gctggcgcaa tggtcatcac
      601 cgaatgcgct aagttctcta ctcgcggtct attccgatca tatctatcgc aaccaaccaa
      661 cgatgatccg cgagaacaaa ccgctgatct cactatgggc acaattgtat atcggcctga
      721 tggtgccacc attaatgctg gcgctactga cgcaggaaaa ggcattagat gtgtcgccgg
      781 aacatttcca tgctgagttt cacgaaaccg gacgcgtcgc ctgtttctgg gtcgatgtgt
      841 gtgaagataa aaacgcaaca ccacattcgc cgcagcagcg aatggaaacg ttaatcagcc
      901 aggcgctggt tccggttgtg caggcactag aagcgaccgg agagatcaac ggcaaactta
      961 tctggagtaa taccggttat ttgatcaact ggtatctcac tgagatgaag caactgctcg
     1021 gcgaggcgac ggttgaatcg ctgcgccatg ccctcttttt tgagaaaacg ctcacgaacg
     1081 gtgaagataa tccactctgg cgtaccgtcg tgctgcgcga tggcctgctg gtgcgccgaa
     1141 cctgctgcca gcgttatcgc ttaccggatg tgcagcaatg tggcgattgt acgctgaaat
     1201 gaaagcactg ccggggaagt aaacccggca tcatgcggat taatagggat gcaacacatt
     1261 acttgcttcg caatgccgca tacataacag aatatctgcg gcctccaaca accctgcatc
     1321 agaactgacg cccagtttcg acatcacatt aaatttatgt gcacggatcg ttttgatatt
     1381 gcgtttaagc tgctcggcaa tttgtgtcat tgagtagcca cgcgacataa agcgcaatat
     1441 ttctctctcc gtcgggctta gcgtccggct ttggttaatg taccactgat tgttcagtcg
     1501 gtcggtcgcc tgacgtacac catttaatga caaaaagagt tcctgatgaa aaatctccag
     1561 cgttgacgct ttacttaata caccgtccag cggtgatggc gacaatgaac caatcagccg
     1621 agcttcaata tcatcatccg caataactaa acgccgggta cgcggaaact taatcgccag
     1681 ttcagtcagg caagataatc cctctctgcg ctcacttctc atggcagaaa aagaaaaaat
     1741 gaccgccgca aatgagatcc gcgacatggc atcctgaaat gactcctgac tggtataggt
     1801 atggagctta taagggttac ccgcaaactt tctgaataaa ctctcaatac cgatactgct
     1861 catgacgcat ttttctatga gtgcgacatt tctcttctta attcggctgt gttccattct
     1921 gcggcatctc cataaacaga aggcaagtta agttctcttg caccctgcgc gctatttatc
     1981 catgcataca tttcagcgtt gctgtgaatt gcc
//
LOCUS       DAEEKQ010000346         2005 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.354, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000346 DAEEKQ010000000
VERSION     DAEEKQ010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.354"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..389
                     /locus_tag="KLK57_004396"
     CDS             <1..389
                     /locus_tag="KLK57_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894575.1"
                     /translation="FDLSLEIPLPPPGILSKTVVPGESSATVKQRVMAARERQFKRQN
                     KLNAWLDSPEIRQFCKLESEDAMWLEGTLIHLGLSIRAWQRLLKVARTIADIDQSDII
                     TRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(414..752)
                     /gene="maoP"
                     /locus_tag="KLK57_004397"
     CDS             complement(414..752)
                     /gene="maoP"
                     /locus_tag="KLK57_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HBE0894576.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            871..1710
                     /gene="hdfR"
                     /locus_tag="KLK57_004398"
     CDS             871..1710
                     /gene="hdfR"
                     /locus_tag="KLK57_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HBE0894577.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(1806..1881)
                     /locus_tag="KLK57_004399"
     tRNA            complement(1806..1881)
                     /locus_tag="KLK57_004399"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1846..1848),aa:Trp,seq:cca)
     gene            complement(1890..1966)
                     /locus_tag="KLK57_004400"
     tRNA            complement(1890..1966)
                     /locus_tag="KLK57_004400"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Asp,seq:gtc)
BASE COUNT          529 a          484 c          487 g          505 t
ORIGIN      
        1 gcttcgatct ctcactggag atcccattac caccccccgg cattttgagt aaaacggtag
       61 tgccgggaga aagcagcgcc accgttaaac aacgcgtaat ggccgccaga gagcgccaat
      121 ttaagcggca gaataaactg aatgcctggc tggatagtcc ggaaatacgc caattctgca
      181 aacttgagag cgaagatgcg atgtggttgg aaggaacact gatccatctg gggttatcga
      241 ttcgtgcctg gcagcggtta ttgaaagttg cacgaaccat tgctgatatt gatcagtctg
      301 acattatcac acgtcagcat ttgcaggagg cagttagcta tcgtgcgatt gaccgtttgc
      361 tcatccatct gcaaaaacta ctgacataaa aaaagggcat ttcgcccttt ttattaatca
      421 tcagaatcgg tgtagtcttc agcaccttca acctgcggtt taccgccgga aagggtgtga
      481 aaacgttttg gacgcttaat acgcgtcata tacttggacc acacgcgttc tgcttctgtc
      541 actggctcac gttcgccacg gcatactgct acgaagagtt tctcttcctc ggtaaccggc
      601 tcgcgtttgc caagatccaa ctcattgaag gcataaccat gacgctcaag cagttgtgcc
      661 tctttgatgg tgaaatcacc atgacgagag aatccacgtg gataatgttt attgtcgaaa
      721 tatcgattag tcgtcgtaaa gctttccgcc atcctgcacg ctcctaattc tttgaccgag
      781 ctagttatgg cgcggagtat tagttacgct tgacagagtg taaaacaaaa catttaaatc
      841 ataacgacaa ataattttgc ggagagcact gtggatacgg aattgttaaa aactttcctg
      901 gaagttagcc gaacgcgtca ctttggtcga gcggctgaat cgctctatct gacccagtca
      961 gcagtgagct ttcgaatcag gcaactggaa aatcaactgg gtgtgaacct tttcacccgc
     1021 cacagaaaca atatccgttt aaccgctgcc ggtgaaaaac tactgcctta tgcagaaacg
     1081 ctcatgagca cctggcaggc cgcccgcaag gaggtggcgc atacctcacg acataacgag
     1141 ttttctatcg gtgccagcgc ctcgttgtgg gaatgtatgc ttaatcagtg gttgggacgc
     1201 ttgtatcaaa atcaggatgc ccatacaggc ttacagttcg aagcgcgaat tgcccaacgg
     1261 cagtctctgg taaaacagct gcatgaacgc cagcttgatc ttcttattac cactgaagcg
     1321 cccaaaatgg acgaatttag tagtcagttg ctggggtatt tcactttagc gctttatacc
     1381 agtgcccctt ctaaactaaa gggagatctt aactatctgc gactggagtg ggggccagat
     1441 tttcaacagc atgaggcagg tttgatcggt gctgacgaag ttcccattct gacaaccagt
     1501 tctgctgaac tggcacagca acagattgcg atgcttaatg gttgcacctg gctacccgtt
     1561 agctgggcgc gtaaaaaagg cggcctgcat accgttgtcg atagcacaac actttcacgg
     1621 ccgctttatg ccatatggct gcaaaatagc gataaaaatg cgttgattcg cgatcttttg
     1681 aaaattaacg tgctggatga agtgtattaa tctgaatggc tggcaaggat gccggtagaa
     1741 ggatttactt cggagaggat tatttcagat aaaaaaaatc cttagctttc gctaaggatg
     1801 atttctggca ggggcggaga gactcgaact cccaacaccc ggttttggag accggtgctc
     1861 taccaattga actacgcccc taattagggt ggcggaacgg acgggactcg aacccgcgac
     1921 cccctgcgtg acaggcaggt attctaaccg actgaactac cgctccaccg aattctttta
     1981 caaccaccgg ttttatgacc ggctt
//
LOCUS       DAEEKQ010000347         2001 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.355, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000347 DAEEKQ010000000
VERSION     DAEEKQ010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.355"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..312
                     /locus_tag="KLK57_004401"
     CDS             <1..312
                     /locus_tag="KLK57_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894578.1"
                     /translation="ALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYS
                     GAGVGYQVASVVGGGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQR
                     A"
     gene            414..1868
                     /gene="amn"
                     /locus_tag="KLK57_004402"
     CDS             414..1868
                     /gene="amn"
                     /locus_tag="KLK57_004402"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HBE0894579.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWNLITADAQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT          479 a          496 c          476 g          550 t
ORIGIN      
        1 gcgcttgaag cacaatctat tttctggata gttttcttct ccataatgct ggcaaacatt
       61 gcgcatgaca tggtggtgtg tgtgcaacaa ccgatgttta ccgaaatgtt tggtgccagt
      121 tatcgctata gtggcgctgg agtcggttat caggttgcca gtgtggttgg cggtggattt
      181 acacctttta ttgccgctgc actcatcact tactttgccg ggaactggca tagcgtcgcc
      241 atttatttgc tggctggatg cctgatttcc gcaatgaccg ctttgttgat gaaagacagt
      301 caacgcgctt gatagcctgg cgaagatcat ccgatcttcg ccttacactt ttgtttcaca
      361 tttctgtgac atactatcgg atgtgcggta attgtatgga acaggagaca cacatgaata
      421 ataagggctc cggtctgacc ccagctcagg cactggataa actcgacgcg ctgtatgagc
      481 aatctgtagt cgcattacgc aacgccattg gcaactatat tacaagtggc gaattacctg
      541 atgaaaacgc ccgcaaacaa ggtctttttg tctatccatc actgaccgta acctgggacg
      601 gtagcacaac caatcccccc aaaacgcgcg catttggtcg ttttactcac gcaggcagct
      661 acaccaccac gattactcgc cctactctct ttcgttcgta tcttaatgaa caacttacgt
      721 tgctgtatca ggattatggt gcgcatatct ctgtgcaacc ctcgcagcat gaaatccctt
      781 atccttatgt catcgatggc tctgaattga cacttgatcg ctcaatgagc gctgggttaa
      841 ctcgctactt cccgacaaca gaactggcgc aaattggcga tgaaactgca gacggcattt
      901 atcatccaac ggaattctcc ccgctatcgc attttgatgc gcgccgcgtc gatttttccc
      961 tcgcacggtt gcgccattat accggtacgc cagttgaaca ttttcagccg ttcgtcttgt
     1021 ttaccaacta cacacgttat gtggatgaat tcgttcgttg gggatgcagc cagatcctcg
     1081 atcctgatag tccttacatt gccctttctt gtgctggcgg gaactggatc accgccgaaa
     1141 ccgaagcgcc agaagaagcc atttccgacc ttgcatggaa aaaacaccag atgccagcat
     1201 ggaatttaat taccgccgat gctcagggta ttactctggt gaatattggc gtgggaccat
     1261 caaatgctaa aaccatctgc gatcatctgg cagtgctacg cccggatgtc tggttgatga
     1321 ttggtcactg tggcggatta cgtgaaagtc aggccattgg cgattatgta cttgcacacg
     1381 cttatttacg cgatgaccac gttcttgatg cggttctgcc gcccgatatt cctattccga
     1441 gcattgctga agtgcaacgt gcgctttatg acgccaccaa gctggtgagc ggcaggcccg
     1501 gtgaggaagt caaacagcgg ctacgtactg gtactgtggt aaccacagat gacaggaact
     1561 gggaattacg ttactcagct tctgcacttc gttttaactt aagccgggcc gtagcaattg
     1621 atatggaaag tgcaaccatt gccgcgcaag gatatcgttt ccgcgtgcca tacgggacac
     1681 tactgtgtgt ttcagataaa ccattacatg gtgagattaa acttcccggt caggctaacc
     1741 gtttttatga aggcgctatt tccgaacatc tgcaaattgg cattcgggcg atcgatttgc
     1801 tgcgcgcaga aggcgaccga ctgcattcgc gtaaattacg aacctttaat gagccgccgt
     1861 tccgataata agttctgaac cttcttatca gaaatagatg tgaaggagta agtaagacac
     1921 ctggcaaata gcctgcaata atcgtgggct gtttgcttcc ttgggcggat acgagtttta
     1981 ttatcgtctt aatgatttcc a
//
LOCUS       DAEEKQ010000348         1967 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.356, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000348 DAEEKQ010000000
VERSION     DAEEKQ010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1967)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1967)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1967
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.356"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(50..193)
                     /locus_tag="KLK57_004403"
                     /pseudo
     CDS             complement(50..193)
                     /locus_tag="KLK57_004403"
                     /inference="COORDINATES: protein motif:HMM:NF024729.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(197..1018)
                     /locus_tag="KLK57_004404"
     CDS             complement(197..1018)
                     /locus_tag="KLK57_004404"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HBE0894580.1"
                     /translation="MFSHPVYLFLREFSLQDSRGGSLSKKAESLSSGKITIGTKEKVI
                     IINELRQCHPLSQLLVIADLPRSTFYYHVKRLNAPDPYQLVKQVILRIYHQHKGRYGY
                     RRIRLACRNESILLNGKTIRKLMKELGISSLIRRKKYRTYRGEQGRTCNNLLKRQFYA
                     DRPNQKWVTDVTEFKVDGRKLYLSPIMDLYNGEIVSYNLTERPLASMVKSMLLDAVEQ
                     LNKDDKPLLHSDQGWQYQMPRWQRWLSDNGITQSMSRRGNCLDNAAMESFFSTLK"
     gene            1007..1967
                     /locus_tag="KLK57_004405"
                     /pseudo
     CDS             1007..1967
                     /locus_tag="KLK57_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     misc_feature    1287..1403
                     /locus_tag="KLK57_004405"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01497"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="AL1L pseudoknot; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /pseudo
                     /db_xref="RFAM:RF01497"
BASE COUNT          495 a          441 c          475 g          556 t
ORIGIN      
        1 cgtaggtgcc caccttttca aatgatctgc gccccaaatc ttggacatcc tatgaaaaat
       61 gtttaagtcg atattgaacc ggactcatgc catcgagttt ctctttaatg cgatggtgat
      121 tgtagtattc aatgtaatca attatatctt gctttagatg atgtaactta ttgtatttat
      181 ttaggtagta acattcttat tttaaagtac taaaaaagct ttccattgct gcattatcta
      241 agcagttacc acgtcgggac atgctttgtg ttatgccatt atcactcaac caacgttgcc
      301 aacgaggcat ctgatattgc cacccctgat cactatgcaa caagggctta tcgtctttat
      361 tgagttgttc taccgcatct aatagcatgc tctttaccat tgatgctaat gggcgctcag
      421 taaggttata tgagactatt tctccattat acaggtccat gattggtgat aaatagagct
      481 tcctgccatc gaccttaaac tctgtgacat ctgttaccca cttttgattt gggcggtcag
      541 cataaaattg tctcttcagt aaattattac aagttcgccc ctgttctcca cgataggtac
      601 ggtacttctt gcgcctgata agtgagctga tacccagttc cttcattaac tttctgatgg
      661 ttttaccatt aagtaaaatg ctctcgttac ggcaggcaag tctgatgcgt cgataaccat
      721 aacgcccttt atgctgatgg tatattctca atattacttg tttaacaagt tgatatggat
      781 ctggcgcatt aaggcgtttc acgtgatagt aaaatgtact acgtggcaaa tccgcgataa
      841 ccagtaattg tgataatgga tgacattgcc ttaactcatt aataatgatt actttttctt
      901 ttgttccaat cgtaattttt cctgagctaa ggctttcagc ttttttagat aatgaaccgc
      961 cccgggaatc ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac
     1021 tcgttttccc ccgaagtccg tcaacgggca gtccgtatgg ttctggaaag tcagggcgaa
     1081 tatgactcac aatgggcggt aatttgttcc attactccaa agattggctg tacaccggag
     1141 actctgcgtg tctgggttcg ccagcatgag cgggatacca ggggcggtga tggagggctc
     1201 accaccgctg aacgtcagcg tctgaaagag ctggagcgtg agaatcgtga actgcgctgc
     1261 agtaacgata tccttcgcca ggcttccgct tattttgcga aggcggagtt cgaccgcctc
     1321 tggaaaaaat aatgccactg ctggataagc tgcgtgggca gtacggggtc ggaccggtat
     1381 gcagtgaact gcatattgcc ccgtcaacgt attaccactg tcagcaacag gacatcatcc
     1441 tgataaacgc agtgcccgtg cgcagcacga tgactggctg aagagagaga tacagcgcgt
     1501 atacgatgaa aatcatcagg tgtacggtgt gcgtaaagtc tggcgccagt tgttacggga
     1561 aggaatcagg gtggccagat gtacagtggc gcgcctcatg gcggttatgg gacttgtcgg
     1621 tgttctccgg ggtaaaaagg tccgcactac cgtcagccgg aaaaccgttg ccgcaggtga
     1681 ccgcgtaaac cgtcagttcg tggcagaacg tcctgaccag ctgtgggtgg ctgattttac
     1741 ttacgtcagc acatggcagg gcttcgtcta tgtggcgttc atcattgatg tgtttgccgg
     1801 atacatcgtg tggtgacgag tctcatcgtc tatggaaacg acattcgtgc tggatgcact
     1861 ggagcaggcg ttgtgggccc gtcgtccgtc tggcaccatc cattacagcg ataaaggctc
     1921 tcagtatgtg tcactggcct ataaggcgcg actaaaagaa gcaaaac
//
LOCUS       DAEEKQ010000349         1959 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.357, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000349 DAEEKQ010000000
VERSION     DAEEKQ010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.357"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            19..519
                     /locus_tag="KLK57_004406"
     CDS             19..519
                     /locus_tag="KLK57_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001343764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0894581.1"
                     /translation="MKRKSLSDTDLNAARRLKEIWMAKKSQLGLTQERAAEIMGFSTQ
                     GAVSHYLNGQTPLNLEAVLKFAGLLKVPPESIRPDMVDLLQIARMYCREPQQDNIVAL
                     PADTEQTEDELPFAVEPMERDLIHTFRAFPKEDQEHMLQEMKDKKESMDRTVARWLAA
                     QKGRRA"
     gene            539..733
                     /locus_tag="KLK57_004407"
     CDS             539..733
                     /locus_tag="KLK57_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HBE0894582.1"
                     /translation="MSTVLSPMVSEFETVEQENSYNEWLRAKVAASLADPRPAIPHDE
                     VMAEMEQIIAQIAAATHRGE"
     gene            733..1023
                     /locus_tag="KLK57_004408"
     CDS             733..1023
                     /locus_tag="KLK57_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012601411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE0894583.1"
                     /translation="MLPVLWLPSARDDLRQIVAYIAKENPAAARRMKIRIETSVLPLT
                     EHPYLYPPSERVLGLREIVTHPNYIILYRVTASSIEVVNIVHSRRQYPGKNS"
     gene            1303..1455
                     /locus_tag="KLK57_004409"
     CDS             1303..1455
                     /locus_tag="KLK57_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HBE0894584.1"
                     /translation="MDTIDLGNNESLVCGVFPNQDGTFTAMTYTKSKTFKTESGARCW
                     LARNTD"
     gene            1455..1832
                     /locus_tag="KLK57_004410"
     CDS             1455..1832
                     /locus_tag="KLK57_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001241301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894585.1"
                     /translation="MRLTMEFKDLPSDVQKTAAHTLHSVLREIGKDIASEPAKDLARK
                     IKTAFVELYNVGTDSETVETKTVSSPIFSLEPSVLSGEICTEISSELLPVIREAIRRR
                     GLDGSYDHDVLQVLRTMVTSLGI"
BASE COUNT          594 a          439 c          456 g          470 t
ORIGIN      
        1 cttattagaa ttggatgtat gaaaagaaaa tcattgtcag acaccgacct caacgccgcc
       61 agaagactga aagaaatctg gatggcaaag aaatctcagt tgggattgac tcaggaacgt
      121 gcggcggaaa ttatggggtt ttcaacacaa ggggcggtaa gtcactacct aaatggtcaa
      181 accccactaa accttgaggc tgttctaaag tttgctggct tactgaaagt tccccctgag
      241 tcaatcaggc cagacatggt agatttgctg caaattgcaa ggatgtattg tcgagaacca
      301 cagcaagata acatagtcgc attaccagca gacacagaac aaacagagga tgaactaccg
      361 tttgccgtag agccgatgga acgagattta atccatacgt tcagagcttt tccgaaagaa
      421 gatcaagaac acatgcttca ggagatgaaa gataaaaaag aatcaatgga tcgtacggtt
      481 gcgcggtggt tagctgcaca aaaaggccgt cgcgcctaaa tcagaggaga tatgtaaaat
      541 gagtacagtc ctttccccaa tggtttcaga atttgagaca gttgagcaag aaaatagtta
      601 caacgaatgg ctgcgggcca aagtagcagc aagcctcgcg gatccccgcc cggcgattcc
      661 gcatgatgag gtaatggcgg aaatggaaca aattattgcc caaattgctg ctgcaactca
      721 cagaggtgaa taatgttacc cgttttatgg ctaccatctg ctcgtgatga tttgcgccag
      781 atcgtagcct acatagctaa agaaaatccc gcggcagcac gcagaatgaa aattcgaatt
      841 gaaacatccg ttttgccgct cacagagcat ccttacctgt atcctccaag cgagcgagtt
      901 cttggtcttc gtgaaattgt aactcatcct aactacatca tcctttaccg agtaacagca
      961 tcaagcatag aagtagtaaa tattgtgcat tctcgaagac agtatccagg caaaaacagt
     1021 taattattcc cgccgacaga accaccttag ggtggttttt tcttgcccca acaaaaaaca
     1081 atagcgatgc tatttacatc aaaaaaaata atgatgctat tgtttcaggc gtcaacccac
     1141 cccgccccac agaatgcagg gcaatacttc gagttaccag acagtggtca ggggttaagt
     1201 agccagcccg aggcgtaaga acatgacggc agggttcaat ctttaactat gcagcaggtt
     1261 ttttgttccg ccaccccggc gttaagggga aatgaggtca gcatggatac tatcgatctt
     1321 ggcaacaacg aatctctggt gtgcggcgtg ttccctaacc aggatggaac gtttaccgcc
     1381 atgacgtata ccaaaagcaa aacgtttaaa accgaatctg gcgcgcgttg ctggttagcc
     1441 agaaacactg actgatgagg ctgacgatgg aatttaaaga tttaccgtct gatgtacaga
     1501 aaacagcagc tcatacattg cattctgtgc tgcgagaaat cgggaaagat attgcaagcg
     1561 agccagcaaa agatctggcc cggaaaatca agaccgcttt cgttgagctt tataatgttg
     1621 gcactgactc tgaaactgtc gagaccaaga ccgtaagttc accaatattc tcacttgagc
     1681 caagcgtatt atcaggtgaa atatgcaccg agatttccag cgaactgctt ccggtaatac
     1741 gagaagcaat tcgccgtcgc ggtttggatg gaagttatga tcatgacgtt ctgcaagttc
     1801 tcaggacaat ggtgacttca ctggggattt gatccctgcg tcctctaagc ttttgatata
     1861 gcggtcctcc aagaagcggt aaattttgtc gaaatcatct tttccctctg gtttattcag
     1921 aggattacag gacccgtaag tagcggcata catttcaac
//
LOCUS       DAEEKQ010000350         1914 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.358, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000350 DAEEKQ010000000
VERSION     DAEEKQ010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1914)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1914)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1914
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.358"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            37..264
                     /locus_tag="KLK57_004411"
     CDS             37..264
                     /locus_tag="KLK57_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HBE0894586.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDTHHGPYE"
     gene            complement(265..639)
                     /locus_tag="KLK57_004412"
     CDS             complement(265..639)
                     /locus_tag="KLK57_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secY/secA suppressor protein"
                     /protein_id="HBE0894587.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGHAAADEWDER"
     gene            complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="KLK57_004413"
                     /pseudo
     CDS             complement(722..1768)
                     /gene="mdtG"
                     /locus_tag="KLK57_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
BASE COUNT          485 a          470 c          474 g          485 t
ORIGIN      
        1 cgttatgccg gtattttttt acggaagagg tatcaaatgc gtttaattgt ggtgagcatc
       61 atggtgacct tgctgagtgg ctgtggcagc atcattagcc gcactatacc ggggcagggg
      121 cacggcaacc aatattatcc cggtgtgcaa tgggatgtgc gtgactccgc ttggcgttat
      181 gtcacaatcc ttgatctgcc attctccctg gtttttgata ctttactgct gccgatcgac
      241 actcatcatg gcccgtatga gtgattaacg ttcatcccac tcatcagctg cagcgtgtcc
      301 ttcctcggta tccagcggtg gctcaagctg aaattccccc tcgtcccatt catgtaatgt
      361 attctcttcc tgccactcct ggcggatctc tatctcatca tagtcgccat caaaaacact
      421 ttgcgcggct tcaccgctaa gcataggtaa acattcacct tcttcccctt cgtcggcaaa
      481 aaactcaact tgccacatga tgtcgccgtc ctgcaaaacg tatttttggg cattgaactg
      541 ttgcacattc gcatcttcgg cgtcgatgcc ggggttgtct gcaagaaatt cttcgcgtgc
      601 agcgtcaatg gcttcttcaa gcgttgcgta catggtcatc acgattctcc ctttgagttg
      661 atgaggtttc agggaaaagg atagctgatt ctccgctttt gcaagtatga aaggcgaaaa
      721 atcagttcga tacctggggt attcgacgac gacgtagact gttccatgaa tagactgcgt
      781 tgaataacac tacgccagcg gtgacgagaa ataccgctct gaaaccgtag ttcgctgaaa
      841 tcgctgctcc catcaatggt ccggtaacgt tgccaatatc acgaaacgat tggttatagc
      901 tgaagatacg tccggcgatt tggttgctcg agttataaac taacagtgtc tgtacggcgg
      961 ggagtagtgc accatcggcg gcaccgagca aaaaacgtaa aatcccaagt tgcaatggcg
     1021 tctgaacgta agacattggg atcaacagca gtacagaaaa gatcagcgcc gtaatcagga
     1081 tcttttcggg tccgattcga tcgccaagtt tgccgagtcg tggtgcactt agcagagccg
     1141 ccacgcctgg caccgaggcg atcatgccac tgataaaggc gacgttactg acgttacccg
     1201 ccagttcgcg gacatacagc gtcagaatgg gggcaattga gcccgtcgcc acctggatga
     1261 ttaacgtagt gacaaacagg ctgagtacca gtttcgggtt tttaagtgat gtcaccactt
     1321 cccgcatgtg cagcatctct tttttgctga tcggctggaa tttttctctt atgcaaaaca
     1381 gggtgacgaa aaagcagagt atgagcacac tggcggtaat aaagaatacc ggacgtaagc
     1441 cgtagctatc ggcgagcagg ccgccagcca ttgggccgag caacgcacca ctaacgccgc
     1501 ctgtggagag cgtacccagc gcccagccgc ttttattacg cggtacttgt gtggcgataa
     1561 gagcattagc gttggggaca aatccgccaa gtaacccaag aagcgcccgc aggatcaaaa
     1621 actgccagat attttgtgcc agccccatca acaccatcac gatgcccatg ccgagggcag
     1681 agcgtaatag catgagtttt cggcctttac ggtcggcgag tccaccccaa aacggtgagg
     1741 cgatggccga aaataaaaat gtaatgctca tatatgctat cttaatgcac tacctgataa
     1801 gtgagcctat acaggttatt gctgtatgtt tatacagtat tattggtaga agactgtgta
     1861 gatttggtat agagatgaat agatactatt aaggtaactt attgaaatca ataa
//
LOCUS       DAEEKQ010000351         1899 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.359, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000351 DAEEKQ010000000
VERSION     DAEEKQ010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1899
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.359"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(89..643)
                     /locus_tag="KLK57_004414"
     CDS             complement(89..643)
                     /locus_tag="KLK57_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HBE0894588.1"
                     /translation="MIYPTNTGKSGEHLRLTTLESVWIQGKLRMWGRWSYIGGGKTGN
                     MFNLMLTSKKLTKTAINEALRRMKKAGLNKSELEAFLRDMINGKQKSWLAHCTDAEAL
                     CIDRVISEVLAEHPGLISVLRQRYEGRGMTKRKMAELLNDAHPKWSLRTCERRIEHWL
                     KVAEFILYKPMVMAFGIEKKVIAF"
     gene            complement(640..930)
                     /locus_tag="KLK57_004415"
     CDS             complement(640..930)
                     /locus_tag="KLK57_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HBE0894589.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHDPETSVLAHYRLAGTCGTAIK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLMHAEGVFRTQEIWRKEGYL"
     gene            complement(930..1529)
                     /locus_tag="KLK57_004416"
     CDS             complement(930..1529)
                     /locus_tag="KLK57_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HBE0894590.1"
                     /translation="MAHIQLIKQTSSGLLLPATPESCDFLHQIKTGEWIHADFKRVRN
                     YAFHKRFFKLLQLGFDYWTPVGGAITPRERELLSGFVDYLCESVGREHTPALSEAAEQ
                     YLNTVATRRTRDTALLKSFEAFREWVTIQAGFYTEHFYPDGSRGRRAKSIAFANMDET
                     EFQQVYKSVLNVLWNWILFRKFSSPEQVENVAAQLLEFA"
     gene            complement(1601..1813)
                     /locus_tag="KLK57_004417"
     CDS             complement(1601..1813)
                     /locus_tag="KLK57_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894591.1"
                     /translation="MEKIKKLFSSKYAVIRRDDLSVIVEMDYFPETKKSMMYRNGRKA
                     IFLPMRVSDIMGNDKLLDELRVRASC"
BASE COUNT          472 a          501 c          387 g          539 t
ORIGIN      
        1 taaagcgggc tctttcaaat gtccatgtct gctattcgcc tcgcggtaca gctttgcgaa
       61 gcgtacaaga attgaagcag tttttacgtc aaaaagcaat aacttttttc tctataccaa
      121 aagccataac cattggtttg tacaaaataa attctgccac ctttagccaa tgctcaatgc
      181 gtctttcaca ggttcttaaa ctccatttcg ggtgtgcatc attcagcagt tcagccattt
      241 tgcgtttggt catcccccgc ccctcatacc gttgccgaag gacgctaatc aatcctggat
      301 gctctgccag cacctcactt atgacccgat caatacataa cgcctctgca tcagtacaat
      361 gcgccagcca gctcttttgc ttaccgttaa tcatatcccg caaaaaagcc tcaagttcag
      421 acttgttcag acctgctttt ttcatcctcc ggagcgcctc gttaattgcc gtttttgtca
      481 gctttttaga ggtcaacatc aggttaaaca tattccccgt cttaccgcca ccaatatacg
      541 accagcgccc ccacatacgt agttttccct gaatccagac actttccagc gtggtgagac
      601 gaaggtgttc tccgcttttt cctgtattcg ttgggtaaat cataaatatc cctcctttct
      661 ccagatttct tgtgtgcgaa aaacaccttc tgcatgcatc aggcgtaatt cttctttggt
      721 gtaatcgctg gtttttaccc gcccgtcgat taaatcgtgg cacgagctac aggcaatcgc
      781 tgcctgcata tcgtgtggct ttatcgctgt tccgcacgtt cccgccagtc ggtaatgcgc
      841 cagcacagac gtttccggat cgtgattgca gtagccagga attctgacgg tgcacatctg
      901 cccccgcgcc gctttacgta aatccaccat tacgcaaact ccagcagctg cgcggccaca
      961 ttttcgactt gttccggaga ggaaaattta cggaacagaa tccagttcca cagcacattc
     1021 agaacagatt tataaacctg ctgaaactcg gtttcgtcca tattcgcaaa agcgatggat
     1081 tttgcccgac gcccgcggct accgtccgga taaaaatgtt cggtgtaaaa tccagcctga
     1141 atggttaccc actcgcgaaa agcctcaaac gactttagca atgccgtatc ccgggttctg
     1201 cgtgtcgcaa ctgtattcag atattgctct gcggcttcgc tcagagctgg cgtatgttcc
     1261 cgacctactg attcgcacag gtaatcaacg aaaccagaca gcagttctcg ttcgcgaggc
     1321 gtgatcgccc caccgaccgg agtccagtaa tcgaatccca gttgcaggag tttgaaaaaa
     1381 cgcttgtgga atgcgtagtt acgcacacgc ttaaagtctg cgtgtatcca ctcacctgtt
     1441 ttgatttgat gcagaaaatc gcaactctcc ggcgtcgccg ggagaagtaa tccggaagag
     1501 gtttgtttga tcagttgtat atgcgccatc gtagttctcc gctggcgcag tagaatgggt
     1561 gttcagcccg ttatgtagta taccagaatt aatgccaata ctaacaggat gctctgactc
     1621 gcaattcatc cagcagttta tcatttccca taatgtcact taccctcatc ggtaaaaaaa
     1681 ttgcctttcg accattacga tacatcattg attttttggt ttcagggaag taatccattt
     1741 cgactataac tgacaggtca tcacgacgta tgactgcgta tttactacta aatagtttct
     1801 ttattttttc cacgatgcct ccaggtttat aagtacaaac ggttatatca acatagagac
     1861 aaaaggtgat gctgccaact tactgattta gtgtatgat
//
LOCUS       DAEEKQ010000352         1844 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.360, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000352 DAEEKQ010000000
VERSION     DAEEKQ010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.360"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            118..660
                     /locus_tag="KLK57_004418"
     CDS             118..660
                     /locus_tag="KLK57_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0894592.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDK
                     ITGNDKAIASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLT
                     DNSVQSGDVLSSLVMRVAQD"
     gene            740..1414
                     /locus_tag="KLK57_004419"
     CDS             740..1414
                     /locus_tag="KLK57_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBE0894593.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWHLTIIDDHGN
                     YISDKI"
     gene            1430..>1844
                     /locus_tag="KLK57_004420"
     CDS             1430..>1844
                     /locus_tag="KLK57_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE0894594.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELE"
BASE COUNT          588 a          326 c          401 g          529 t
ORIGIN      
        1 tttcaaataa ataaaacaat gtgtttacat tgcggtaact tttttgatgt gagaaatttg
       61 tttccaagag atacagactc ttaacaacca aaaaaaaatt ttattaagga acatattatg
      121 aaacgttcaa ttattgctgc cgctgtcttt tcttcttttt ttatgagcgc tggagtattt
      181 gctgcagacg ttgataccgg aacattaact attaagggga atattgcaga atctccgtgt
      241 aaattcgaag cgggtggtga ttcagtaagt attaatatgc cgactgtacc aaccactgtc
      301 tttgaaggta aagctaaata ttctacctat gatgatgcag tcggtgtaac cagcagcatg
      361 ttaaaaatta gctgcccgaa agaagttgct ggtgtaaaac tctcgttgat taccaacgat
      421 aaaataaccg gtaacgataa ggcgatagcc agtagcaacg ataccgtggg ttactatctc
      481 tatttaggtg ataacagcga tgtcctggat gtttctgcac cttttaacat tgagagttat
      541 aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat
      601 aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa
      661 taaataaaat tgtaagtttc tccaggtaat aaacatggcc gctatcccat ggcggccttt
      721 taatttaaga ggcattaaaa tgaaaggatt attatcttta ctcatttttt ctatggtcct
      781 tcctgcacat gccggaattg ttatctacgg gacgcgcatt atttacccgg cagaaaataa
      841 agaagtgatg gtgcagttga tgaaccaggg aaaccgttct tcgctgctgc aggcgtggat
      901 tgatgatggc gatacgtcat taccaccaga aaaaattcag gttcctttca tgttaacgcc
      961 accagtggca aaaatagggg caaattccgg gcagcaagta aaaatcaaaa ttatgccgaa
     1021 taaactgccc actaataaag aaagcatttt ttatctgaat gttctggata ttccaccaaa
     1081 tagtccagag caagaaggta agaatgcact gaagtttgcg atgcaaaaca gaattaagtt
     1141 gttttaccga ccagcgggta ttgctccggt aaataaagcg acatttaaaa aattgctggt
     1201 aaatcgcagt ggcaatggtt tggtgataaa aaatgactca gctaattggg tgacgatttc
     1261 ggatgtcaaa gctaataatg tcaaagtcaa ttatgaaact attatgattg cccccttaga
     1321 aagtcagagt gttaatgtca aaagtaataa tgcaaataac tggcatctga ccattatcga
     1381 tgaccatggc aactatatta gtgacaaaat ttaattgcag gagctgccta tgttgagaat
     1441 gaccccactt gcatcagcaa tcgtagcgtt attgctcggc attgaagctt atgcagctga
     1501 agaaaccttt gatacccatt ttatgatagg tggaatgaaa gaccagcagg ttgcaaatat
     1561 tcgtcttgat gataatcaac ccttaccggg gcagtatgac atcgatattt atgtcaataa
     1621 gcaatggcgc gggaaatatg agattattgt taaagacaac ccgcaagaaa catgtttatc
     1681 aagagaagtt atcaagcggt taggcattaa tagcgataac ttcgccagcg gtaagcaatg
     1741 tttaacattt gagcaacttg ttcagggtgg gagctatacc tgggatatcg gggtttttcg
     1801 tctcgatttc agtgtcccgc aggcctgggt ggaagaactg gaaa
//
LOCUS       DAEEKQ010000353         1826 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.361, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000353 DAEEKQ010000000
VERSION     DAEEKQ010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.361"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..618)
                     /locus_tag="KLK57_004421"
     CDS             complement(<1..618)
                     /locus_tag="KLK57_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0894595.1"
                     /translation="MKRLQAFKFQLRPGGQQECEMRRFAGACRFVFNRALARQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFRKRAAFPR
                     FKKRGQNDVFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPAHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQK"
     gene            626..1127
                     /gene="tnpA"
                     /locus_tag="KLK57_004422"
                     /pseudo
     CDS             626..1127
                     /gene="tnpA"
                     /locus_tag="KLK57_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(1145..1813)
                     /locus_tag="KLK57_004423"
                     /pseudo
     CDS             complement(1145..1813)
                     /locus_tag="KLK57_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
BASE COUNT          465 a          437 c          419 g          505 t
ORIGIN      
        1 tttctgaaaa ctgtttacag gctcaaagac tgtgccatct gacagcgtgg cgagtttagc
       61 cacgccagca tccagcccga ccattgatgc tgaagggtga gccggagtgg atacttcact
      121 ttctgtctga atactgatgt accacgtacc gcaggactgg ctgacagtga catttttcac
      181 aacacccgtg acctgacggc tattccggta gcgcatccag cccagtttcg gcagaaaaat
      241 acggctgttt tcctgatcga gcttaacgcc ctgcgggtag cggaatacat cattctgtcc
      301 ccgctttttg aatcggggaa aagcagcccg cttccggaag aagtttttgt aagcccgctc
      361 aaggtctttc agtgactgtt gcaatggctg tgagggagaa tctttaagcc attgcgtttc
      421 agtggcattt ttccactcaa ccagccagga agccattttc ccgtaaggga tgtatttatt
      481 accggcctca tgattctcat tctgacgtgc cagagcacga ttgaaaacga aacgacatgc
      541 gcctgcgaag cgcctcattt cacactcctg ttgaccacct ggtcttaact ggaatttaaa
      601 tgcctgtaat cgtttcacag gtcaaatgat actttggtct gtgaaaaaag aaaccgatat
      661 ccggcgtgga aggcattgtg ttttcctgat gcatgttcac ctggtctttg tcactagata
      721 ccgacgccag attttttgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      781 cttattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc catttgttaa
      841 ttaactaacc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      901 gtaggttact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      961 tgtggagtcc tggctatttt gccagtagct gcggaggtgt gccaatatcc gtcatccgcc
     1021 aatacattga acaacagcaa acacctggtc aggtggaaaa ccgcgcctta tatccccgcc
     1081 ctgaaggatg gggttttacg gcgcaccgga taaaaagcgc catctaacga tggcgcgtct
     1141 ggaactactt tttgttaacg tcaaacatgg tggcatcggt cgccatgtca tcaataactt
     1201 gtttaatatt ttcgaaagcc attggcgtac tttcgttatt caggtcttta ccttcgccct
     1261 gacgaacgac tttgataacc ggtttattag tcgctgcatc aatcagctca ccttcaaaat
     1321 agagtcgagt atccatggta cggtggcctg tagccatttg cgtccccgca accactaatg
     1381 caacaggaac cacttcatag aattgcagcc cttctttgct ggtatctaca ccggtaatgg
     1441 ccccacggaa aatcagacta cgcggcccag cggtggtaac cagtggttta cgctgcgcta
     1501 tcgcttcttt catttcggtg ttggtatagt tcaaaatttt atccagaact ttctgcccta
     1561 cctaggtaga aggttttggc accggataat aagtgattgg gttccagacg atgctgtcat
     1621 atttgctttg atcaaaactc gggtctaccc aacgtaaaac aggtttacct gttgctgagg
     1681 ttgtttcttt taaatcagag taattgttta aaaaaccaga atatttatct ggctgggtga
     1741 ttttagaggc acaaccagac agagccaaca agccgcataa agctgcaact ttcgcaaatg
     1801 atgtggtacg catgaatagt ttcctg
//
LOCUS       DAEEKQ010000354         1818 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.362, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000354 DAEEKQ010000000
VERSION     DAEEKQ010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1818
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.362"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(8..859)
                     /gene="hchA"
                     /locus_tag="KLK57_004424"
     CDS             complement(8..859)
                     /gene="hchA"
                     /locus_tag="KLK57_004424"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000218213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HBE0894596.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKVLTGDSPFAANALGKLAAQE
                     MLAAYAD"
     gene            1115..1310
                     /locus_tag="KLK57_004425"
                     /pseudo
     CDS             1115..1310
                     /locus_tag="KLK57_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1450..>1818)
                     /locus_tag="KLK57_004426"
     CDS             complement(1450..>1818)
                     /locus_tag="KLK57_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBE0894597.1"
                     /translation="AMMYAETRNMTPYGNVGIANKTQNFEAVAQYQFDFGLRPSLAYV
                     YSKGKDLGGNDYNNNGHQEYVDQDLVNYVEIGATYYFNKNFSTYVDYKINLLDKDDDF
                     YDNNGIATDDVVGVGLVYQF"
BASE COUNT          478 a          370 c          445 g          525 t
ORIGIN      
        1 acagggatta atccgcataa gctgccagca tttcctgcgc cgccagttta cccaacgcat
       61 tcgctgcgaa aggactgtcg ccggtgagaa ctttacggtc cttatgtact cgcccggtga
      121 tgtcgtcatt aataatgttc atgcccattt tcttcagctc ttcgccgaag taccaggtga
      181 gatgacccgg catatagcct atctctggcg tttgtttgtc tgcggcgtct gggaatgcgc
      241 aaatggaata accattcagt gggttatcgc cgtggcgaag cgccagaaaa gccgccgggc
      301 cgtggcaaag ggagataaca aaacggtcat ttttgattgc ccactgcaaa gcagcagcta
      361 cgtcctggct ttctggtaaa ccaataagtg cgccatgacc accaggaaca aagattgctg
      421 catattcgct atcagcgttg aggctggcga caacatccgc gagtttcttc ggattgcgga
      481 acaacgattt atgctgctca aagaatggca ttactttttc atctttgtgc ggcatagccc
      541 agtattcaaa cttggtcatc agaccggaaa tggtcgccac ttcgaattcg aaaccagcag
      601 catggagatg atacaacggc agcaacgttt caatcggatg gttaccggtc gagaacagtt
      661 ttccgttatc ggttggcaaa taacgttcgt ccgcggcgat caccagaatt ttatgcttac
      721 cgcgatacgg ttttggatag tccacgccat caagatcaga gacgggactg gtatattggc
      781 taagcgaata ttctgaaggg aagaatgcat tatcttcagc aatatcgacc tgcggatttt
      841 tacttgtttg aacagtcata gtgtattcct tattgttgct tagttagggt agtcactata
      901 tttgcgactg agctggtcgc tatttttaac cagtctggaa aattgagtca atatatcgaa
      961 agcggtagtc ggcgggtgta agtatgtaat gatgatgctg cactggtgtt gaatattatt
     1021 cacgccaaat tttccttatt gagcaacgtc gtcgcaagtg gcctcaatga ttttaggcca
     1081 ttatcaaagg tacggggcgg cggggagaga tttagtgctg tgaaatattg tggtactgat
     1141 gaatatttat atcgcggatt ggtagagaaa agattatttc atgcactatt tttgcaaata
     1201 tttctatatg ggaagcgtgt ttgttacgga ttaattcata aagaaatgca tttgggtgtt
     1261 ggtttgaaga gtcgctggtt gatgttattg tgccaggagg ggcagggtaa ggtgctgtat
     1321 cgatatgcat tggcaattgc gtgtgccgca gatggagatg aaatgccaat aaaaaagcct
     1381 gaattcgatg agggacgaac tcaggctagt gtttagtttt acagtaacac gccgcagcac
     1441 gttggctgat tagaactggt agactaagcc cacaccaaca acgtcatctg ttgcgatgcc
     1501 attgttgtca tagaaatcgt catctttatc caacaagttt attttatagt caacatatgt
     1561 tgagaagttc ttattgaaat agtaggtcgc tccaatttca acatagttga ccagatcctg
     1621 atcaacatac tcctgatgac cattgttgtt atagtcgtta ccgccgaggt ctttgccttt
     1681 tgaataaaca tatgcaagag atgggcgcag accaaaatcg aactgatact gtgctacagc
     1741 ttcaaagttc tgggttttat ttgcgatacc cacattacca tatggtgtca tattgcgggt
     1801 ttcagcatac atcattgc
//
LOCUS       DAEEKQ010000355         1802 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.363, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000355 DAEEKQ010000000
VERSION     DAEEKQ010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.363"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            92..643
                     /locus_tag="KLK57_004427"
     CDS             92..643
                     /locus_tag="KLK57_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HBE0894598.1"
                     /translation="MSAFKKSLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIK
                     PGDEDLTVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTM
                     TGQTDLLKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARM
                     ESPGKDATPGNVYAQADYKIAYE"
     gene            703..1710
                     /locus_tag="KLK57_004428"
                     /pseudo
     CDS             703..1710
                     /locus_tag="KLK57_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
BASE COUNT          576 a          365 c          351 g          510 t
ORIGIN      
        1 aaagtaaaat ataatatttg aatgattagc aatccttaaa taacagttac taatcattca
       61 ttttctactt atatttatat aaaggaatat tatgtctgct tttaaaaagt cacttcttgt
      121 tgctggcgta gcaatgattc tttccaataa cgtatttgct gacgaaggtc acggcattgt
      181 taaatttaaa ggtgaagtta tctctgctcc atgttcaatt aaaccaggtg acgaagactt
      241 aactgtaaat ctgggtgaag tagcagatac cgttctgaaa agcgaccaga aatctttggc
      301 tgagcctttc actattcacc tgcaagactg catgttgagc cagggtggaa ccacttattc
      361 aaaagcgaag gttactttca ccacagcgaa tactatgacg ggtcagactg acctgctgaa
      421 aaacaccaaa gaaactgaaa tcggtggtgc cacaggcgtt ggcgtacgta ttctggatag
      481 ccagagcggc gaagtgactt taggtactcc ggtagtaatt acattcaaca atactaacag
      541 ctatcaggaa ctgaacttca aagctcgtat ggaatccccg ggcaaagatg ctaccccggg
      601 caacgtttac gctcaggctg actataagat tgcttacgag taatcacatt aggccgggtt
      661 cgcccggcca catcattaac ggattaatga taagtggaac agatgtataa aaaattaaaa
      721 ttaaccacaa taagcgaatt gattaaaaat atttattgtt cattatccgt tctcatcatt
      781 ggttgtgcgt cagcttatgc cgttgaattc aacaaagatt taatcgaagc cgaagatcgt
      841 gaaaacgtta acctttccca atttgaaact gatggccaat tacccgtagg taaatactca
      901 ttaaacgctc ttattaataa taaaaggacg ccaatccatc tggacctgca atgggtatta
      961 attgataacc aaactgcagt ttgtctgact ccagagcaat taacattatt aggctttact
     1021 gatgagatta ttgaagaggc tcagcaaaac ctgatcgatg gttgttaccc tatcgaaaaa
     1081 gaaaaacaaa ttacaactta tctcgataaa gggaaaatgc aattatccat atctgcacct
     1141 caggcatggt taaaatacaa agatgcaaac tggacgcctc ctgaactttg ggatcatggt
     1201 attgctgggg catttcttga ctacaattta tatgcctctc attatgcacc acatcagggc
     1261 gataattcac aaaatataag ttcctatggg caggctgggg ttaatcttgg ggcctggcgc
     1321 ctgcgtactg attaccagta cgatcagtca tttaacaatg gcaaaagcca ggcgaacaac
     1381 ctggattttc cgcgtattta tttgtttcgc ccaatcccag caattaatgc aaaactaact
     1441 ataggtcaat acgatactga atcctctatt ttcgactctt tccatttttc tggcgtttcg
     1501 ttgaaaagcg atgagaatat gttgccgcct gatctacgtg gttacgcacc gcaaatcacg
     1561 ggtgttgcac aaacgaatgc aaaggtcact gtctcacaga acaaccgtat tatttatcaa
     1621 gaaaatgttc ctcctggccc atttgctatt actaatttat tcaatacatt acaggggcaa
     1681 ctggacgtca aggttgaaga agaggacggg ccagtctata tatcaatgac actaaccatt
     1741 acatgaatta tcagaatgaa aaagagagtt atattgtcac gtttaaccag tatcttgaat
     1801 ca
//
LOCUS       DAEEKQ010000356         1764 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.364, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000356 DAEEKQ010000000
VERSION     DAEEKQ010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1764)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.364"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            17..544
                     /locus_tag="KLK57_004429"
     CDS             17..544
                     /locus_tag="KLK57_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE0894599.1"
                     /translation="MAVRGINKVILVGRLGKDPEVRYIPNGGAVANLQVATSETWRDK
                     QTGEMKEQTEWHRVVLFGKLAEVAGEYLRKGAQVYIEGQLRTRSWEDNGITRYVTEIL
                     VKTTGTMQMLGSAPQQNAQVQPQPQQNGQSQSADATKKGSAKTKGRGRKAAQPEPQPQ
                     PPEGEDYGFSDDIPF"
     gene            602..835
                     /locus_tag="KLK57_004430"
     CDS             602..835
                     /locus_tag="KLK57_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HBE0894600.1"
                     /translation="MSEYFRILQGLPDGPFTRKQAEAVAAQYRNVFIEDDHGEQFRLV
                     VRNNGTMVWRTWNFEDGAGYWMNHVIRDFGILK"
     gene            896..>1764
                     /locus_tag="KLK57_004431"
     CDS             896..>1764
                     /locus_tag="KLK57_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HBE0894601.1"
                     /translation="MSVTKSEPKSTRKASRKPAKTQETVLSALLAQTEEVSVPLASLI
                     KSPLNVRTVPYSAESVSELADSIKGVGLLQNLVVHALPGDRHGVAAGGRRLAALNMLA
                     ERGIIPADWPVRVKIISQELATAASMTENGHRRDMHPAEQIAGFCAMAQEGKTPAQIG
                     DLLGYSPRHVQRMLKLADLAPVILDALAEDLITTEHCQALALENDTARQVQVFEAACQ
                     SGWGGKPEVQTIRRLVTESEVAVAGNSKFRFVGADAFSPDELRTDLFSDDEGGYVDCV
                     ALDAALLEKLQAVA"
BASE COUNT          418 a          466 c          545 g          335 t
ORIGIN      
        1 cagaaggaga cacatcatgg cagttcgtgg cattaacaag gtcattcttg tcggtcgtct
       61 gggaaaagat ccggaagtcc gttacatccc caacgggggc gcagtggcaa acctgcaggt
      121 ggccacgtca gaaacctggc gcgacaaaca gacgggggaa atgaaggagc agacggaatg
      181 gcaccgcgtg gtgctgttcg gcaagctcgc ggaagtggca ggtgaatatc tgcgtaaggg
      241 ggcgcaggtc tacattgaag gccagcttcg cacccgtagc tgggaagata acggtattac
      301 ccgttacgtc accgaaatcc ttgttaaaac cacgggcacc atgcagatgc tggggagtgc
      361 accacagcag aacgctcagg tgcaaccgca gcctcagcag aatggtcagt cacagagtgc
      421 tgacgcgacg aaaaaaggga gcgcgaaaac gaaaggccgt ggacgtaagg ctgcgcagcc
      481 ggagcctcag ccgcaaccac cggaggggga ggattacggg ttttcagacg atatcccgtt
      541 ctgactggcc tgactgtgat aaccgccccg tcctgcgcgg ggcatcaccg gagatatgag
      601 gatgagcgaa tatttcagaa tacttcaggg actgcctgac ggtcctttta cccgcaaaca
      661 ggccgaagcc gttgccgcac agtaccggaa cgtctttatc gaggatgacc acggcgaaca
      721 gtttcgcctg gttgtccgta ataacggaac aatggtctgg cgtacctgga attttgagga
      781 cggtgccggg tactggatga accatgtcat ccgtgatttc gggatcctta agtaagagag
      841 gtgccggacg gggatcacgt ccggcagaca ttaactacac aataagggga tagtcatgtc
      901 agtaacaaag tctgaaccaa aatctacccg taaagcaagc cgtaaacctg caaaaacgca
      961 ggaaactgtc ctgtctgccc tgctggcgca gacggaggaa gtgagcgtgc cgctggcctc
     1021 gctgattaag tcaccactga atgtgcgcac ggtgccgtat tctgcggagt ccgtcagcga
     1081 actggctgat tccattaagg gggtcggcct gctgcagaat ctggttgttc atgccctgcc
     1141 aggtgaccgc cacggtgtcg ccgcaggtgg tcgccgactg gcagcactca acatgctggc
     1201 agagcgtggc atcattccgg ctgactggcc tgtccgcgtg aaaattattt cgcaggaact
     1261 ggcgacggcc gcatcgatga ccgagaacgg tcatcgtcgg gatatgcacc cagccgaaca
     1321 gattgccgga ttctgtgcaa tggcgcagga aggcaaaaca cctgcacaaa tcggtgattt
     1381 gctgggctat tcaccccgcc acgttcagcg aatgctgaaa ctggctgacc ttgcgcctgt
     1441 catcctcgat gcgctggcag aagacctcat caccaccgag cactgtcagg cgctggcgct
     1501 ggagaacgac accgcgcgtc aggtgcaggt gtttgaagcc gcctgtcagt cgggatgggg
     1561 cggtaaaccg gaagtacaga ccattcgtcg tctggtgacc gaaagtgaag tggcggtggc
     1621 agggaacagt aaattccgct tcgtgggggc tgatgccttc tcgccagatg aactgcgcac
     1681 tgacctgttc agcgacgacg agggtggcta tgtggactgc gtggcgctcg atgccgccct
     1741 gctggaaaaa ctccaggctg ttgc
//
LOCUS       DAEEKQ010000357         1704 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.365, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000357 DAEEKQ010000000
VERSION     DAEEKQ010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1704)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.365"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..165
                     /locus_tag="KLK57_004432"
     CDS             <1..165
                     /locus_tag="KLK57_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000885581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE0894602.1"
                     /translation="SEYIAPLQDAVDLEIATEEERSLLEAWNKYRVLLNRVDTSTAPD
                     IEWPANPVRE"
     gene            280..450
                     /locus_tag="KLK57_004433"
     CDS             280..450
                     /locus_tag="KLK57_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001710241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894603.1"
                     /translation="MVRLPPVVMSAKSATARTTIAREDAEDHQVKLSAQKLEELLASM
                     VKDEVDRNDGIY"
     gene            complement(577..1134)
                     /locus_tag="KLK57_004434"
     CDS             complement(577..1134)
                     /locus_tag="KLK57_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001530027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HBE0894604.1"
                     /translation="MNTAITFSNESPKVFFKDGRLVTTSQAVADYFDKQHKHVLAKID
                     TLDCSPEFTSANFSADVQTVEIGNGAERESRCYLITKDGFMFLVMGFTGKKAARLKEA
                     YIEKFNAMEEELHKRPPAAQNSPAPNNGCALLIHFDKHGQVEFTEKVPADAMVCTLER
                     FKFYLEQHGWIVARKEQLVERLMRF"
     gene            complement(1131..1406)
                     /locus_tag="KLK57_004435"
     CDS             complement(1131..1406)
                     /locus_tag="KLK57_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HBE0894605.1"
                     /translation="MTVFVLRGYIPHTTAKSVVGIGVSDNITTHSRVCGFFCVKHCHI
                     RIMVGRAGAEKSAPGFVMTGYANPVRLTTSLIGVRGGDHPINWSHHI"
     gene            1591..1704
                     /locus_tag="KLK57_004436"
                     /pseudo
     CDS             1591..1704
                     /locus_tag="KLK57_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="receptor for colicin S4; incomplete; partial in the
                     middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
BASE COUNT          474 a          354 c          419 g          457 t
ORIGIN      
        1 agtgagtata ttgcgccact gcaggatgct gtagatctgg aaatcgcaac ggaggaagaa
       61 agatcgttgc tggaggcatg gaataaatat cgggtattgt tgaatcgtgt tgatacatca
      121 actgcacctg atattgagtg gcctgcaaat cctgtcaggg agtaatcatt gggattatgc
      181 cgcagacacg tcgtatgcag gaacgtgctg cggttagttt gtgagctttc gatagtggtt
      241 gttatttttg cccttatttg ttccggaggc catggttcaa tggtccgtct gccccctgtg
      301 gtgatgtcag caaaatcagc cactgcgcga accacaatag cccgggaaga tgctgaagat
      361 caccaggtaa agctgtcagc gcagaaactg gaagaactgc tcgcatcaat ggttaaggat
      421 gaggttgatc gcaatgatgg gatttattga cgtcagcgag agcagaagga agaactgaat
      481 aacctgaatg atttacgctc aatcagagcg atggcgatta gcggcaatca cgctcgataa
      541 aattaacctc ggtgatcatc cggggttttg cattcattaa aaccgcatca acctttccac
      601 caattgttct ttacgggcaa cgatccaccc atgttgctcc agataaaatt taaaccgttc
      661 cagagtgcat accatcgcat cggcggggac tttttctgtg aactcgacct gaccgtgttt
      721 atcgaagtgg atcagtaatg cgcatccatt atttggggcg ggggagtttt gtgctgcagg
      781 aggacgtttg tggagttctt cttccattgc gttaaatttt tcaatgtaag cttcttttag
      841 tctggctgct tttttgccag taaatcccat tacgaggaac atgaagccgt cttttgtgat
      901 caggtagcat cgtgattcac gttctgcgcc gttgccaatt tcgacagtct gaacatcggc
      961 cgaaaagttg gctgatgtaa attcgggaga gcagtcgagt gtgtcaatct tagctaaaac
     1021 gtgtttatgt tgtttatcga agtagtcggc aacagcttga gaggtggtaa ccaggcgacc
     1081 atctttgaaa aagacttttg gtgactcgtt agaaaaagtt atagctgtat tcatatgtga
     1141 tgactccaat taattggatg atcaccacct ctgacgccaa tcagggaggt ggtgagacgt
     1201 acagggttgg cgtaaccggt catcacaaaa cccggcgcgg atttctccgc ccctgcacgc
     1261 cccaccataa tgcgaatgtg gcaatgctta acgcaaaaaa aaccgcaaac gcggctatgc
     1321 gttgtgatgt tatccgagac gccaatcccg acaaccgatt ttgcggttgt gtgaggaata
     1381 tagccccgga gaacaaaaac tgtcaacggg caaaaaaatt tccgttatca ggaatgtata
     1441 aaattaaagc gatcgagcaa aaaatccttt ctgcaaaact aaaaaagatt tcaataaaat
     1501 caggcagtta aaaacacgga aaacggtata aaaaagcgcc tcaaaatcct gttttttctt
     1561 tcagatacaa tgtcttattg cattttttaa aacatgtcag tgtggtacat ggatatcgat
     1621 accatcgcca attataaact gggcggtgca cagcaacatg atagcgtacg cctcgatccg
     1681 tgggtgttta tgttctcagc agga
//
LOCUS       DAEEKQ010000358         1703 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.366, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000358 DAEEKQ010000000
VERSION     DAEEKQ010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1703
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.366"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>1703
                     /locus_tag="KLK57_004437"
     CDS             <1..>1703
                     /locus_tag="KLK57_004437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HBE0894606.1"
                     /translation="VYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEI
                     ITPKKPGEAILHAFNATYQQIRENMSEFARCHYGYIQIPPVTTFRADGPETPEEEKGY
                     WFHAYQPEDLCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDY
                     PSADIRAKISKDIEGGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKD
                     NISKDEWENLHALKEKRLNGIPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHS
                     KHEQIERSITPPLKNYNERLHNTNLFNPKYHKKAVMNYMEYLVTCQLDEQQMSSLLYE
                     RDDLSAIDFSLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGN
                     LFNDFLFNHQQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGSTRVGGEAWL
                     NKGCSTYQQLYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVI
                     INKGNEEVEGTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPGKVFTLRGGTLNINIP
                     G"
BASE COUNT          592 a          333 c          317 g          461 t
ORIGIN      
        1 atgtatataa tattaacgtt acgtcacctt ccggacagca agaaacattt aaaatactaa
       61 tcaatccaga acatctacgg cagacacttg agcgtaaatc tcttactgct gttcagaaat
      121 cacaatgtga aatcatcacc cctaaaaaac ccggcgaagc gattcttcat gcttttaatg
      181 ccacctacca gcaaatcagg gaaaatatgt ctgagtttgc acgttgccat tatgggtata
      241 tacaaatccc tccagtgaca actttccgcg ccgacggacc agaaactccc gaagaagaaa
      301 agggttactg gtttcatgct tatcaacccg aagatctttg taccatccat aatccaatgg
      361 gagatttgca ggattttatt gcattggtta aagatgctaa aaaatttggt atcgatatca
      421 ttcctgatta tacctttaac tttatgggaa ttgggggtag tggtaaaaat gacctggatt
      481 atccctctgc tgatatacga gcgaagatca gtaaagatat agaaggtggt atccctggct
      541 attggcaagg tcaggttttg attccattca ttaaagatcc agtaacaaaa gaacgtaaac
      601 aaatccatcc agaagatata catctcactg caaaagactt cgaagcaagt aaagataaca
      661 tctctaagga tgaatgggaa aacctccatg cattaaaaga aaagcgttta aatggaattc
      721 ctaaaacaac acccaaaagt gaccaggtta ttatgttgca aaatcaatac gttcgtgaaa
      781 tgcgaaaata tggcgtacga ggtttacgtt atgatgcggc aaaacactca aaacatgaac
      841 aaatagaaag atcaataacc ccaccgctta aaaattataa tgagcggtta cacaatacta
      901 acttatttaa cccaaaatat cataaaaaag ccgttatgaa ttacatggaa tatctggtaa
      961 cttgtcagtt ggatgaacaa caaatgtcat cactgcttta tgaaagagat gatttaagcg
     1021 ccattgattt ttcattgctc atgaagacga taaaagcctt ttcattcggg ggagatctcc
     1081 taacccttgc atcaaaaccg ggttccacaa tctcaagcat cccatcagaa agacggatat
     1141 tgattaacat taaccacgat tttcctaaca atggcaatct tttcaatgac tttctattta
     1201 accatcaaca agatgaacaa ttagcaatgg catatatggc cgctctcccg ttcagcagac
     1261 ctttagttta ctgggatggc caggtattaa aatcaacgac tgaaattaaa aattatgatg
     1321 ggtccacgcg tgtcggtggt gaggcgtggc ttaataaagg ttgctctacc tatcagcagc
     1381 tctacaatga attccacgca ttatatatag ataaagcagg aatatggagc gcatttgagg
     1441 gtgtatttgc aactaaaaac gttctagcct ttagtcgtgg ggattctgtg aacattaatc
     1501 actctcctca tgatggacta gttataataa ataaaggaaa cgaagaagtt gaaggtacct
     1561 ggcctaacaa attgcaacct ggaatatata aaaacatggg gagtaatagc gttaacatta
     1621 ttattaataa tacccgaaaa attatccccc ctggtaaagt atttacgctt agaggcggaa
     1681 ctctaaatat caatattcct ggg
//
LOCUS       DAEEKQ010000359         1657 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.368, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000359 DAEEKQ010000000
VERSION     DAEEKQ010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1657
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.368"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            84..641
                     /locus_tag="KLK57_004438"
     CDS             84..641
                     /locus_tag="KLK57_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894607.1"
                     /translation="MQYSEFRAMLQASRERNKHNSYAYTNNPTSYEVPTFSKSERKNI
                     EAVIRSITPRDRFMPVRKTTKNTIKNYLANFDSYEQLPSKLDDVFIGFCRSEGHPKYN
                     KKLFYLLKNLDEINASTVTNHLQRQATRLSYELPSDKYCALLAVMCAKLIGIVEHHIA
                     VGNIEPMENEQPDFEFDPYLVTEDF"
     gene            642..920
                     /locus_tag="KLK57_004439"
     CDS             642..920
                     /locus_tag="KLK57_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894608.1"
                     /translation="MNKHTLTRVTKTAFDELNRIKNITGLPHSAVMKLAINRDINEQD
                     LLNSRLEAARHHIRIKQDDLDSLKSLNAFGLDLARLLSIKIHKLSQEV"
     gene            923..1195
                     /locus_tag="KLK57_004440"
     CDS             923..1195
                     /locus_tag="KLK57_004440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HBE0894609.1"
                     /translation="MKQWRDDIKRFFATYFMINYETGMLEWIDGGEVKTRDDAYGNPM
                     IRYGTRDYYLKYVIWFLHHEVQATKKIIFRDGNKRNCHPNNLLLEI"
     gene            1195..1395
                     /locus_tag="KLK57_004441"
     CDS             1195..1395
                     /locus_tag="KLK57_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001597778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894610.1"
                     /translation="MLCIKFEYLTDKMIKHVSDLLIKEGGFGDACNPKDIFIHATSPN
                     ATLKTAVTAEWFERNKAELGYW"
BASE COUNT          571 a          301 c          332 g          453 t
ORIGIN      
        1 aatacttgac attagtcttt gaaaatgcta atattgtata taggttaaca gaataataac
       61 atcttatgag agtcagaaca tgaatgcagt attcagaatt tagagcgatg cttcaggcat
      121 caagagaacg caacaagcac aactcctacg cttacacaaa caaccccacc agctacgaag
      181 tcccaacctt tagcaaatcc gaacgtaaga acattgaagc ggtcattcgt agcattacgc
      241 cacgagacag atttatgcca gtacgcaaaa caacaaagaa tactattaaa aattatctgg
      301 ctaactttga cagttacgag cagttaccaa gcaagcttga tgatgttttt attgggttct
      361 gtagaagtga aggccacccg aaatacaaca aaaaactatt ttatctgcta aagaatcttg
      421 acgaaattaa cgcatccacc gtgacaaatc atcttcagcg acaggcaacc agacttagtt
      481 acgaattacc aagcgataaa tattgcgctt tgcttgctgt gatgtgcgcg aaattaattg
      541 gcattgttga acatcatatt gccgttggga atattgagcc aatggaaaac gagcaaccag
      601 atttcgagtt tgacccctat ttagttacag aggattttta aatgaataaa cacacattaa
      661 ccagagttac aaagacagcc tttgatgaac tgaaccgaat caaaaacatt acaggtctgc
      721 cacactcagc agtgatgaag cttgcaatta accgtgatat taacgaacag gatttgctta
      781 atagtcgctt agaggctgca agacaccata tcaggattaa gcaagatgac cttgatagtc
      841 tgaaatcctt aaatgctttt ggactggatt tagcaaggtt actaagtatc aaaattcaca
      901 aactgtctca ggaggtctga ttatgaagca atggcgagat gatattaaac ggttctttgc
      961 aacttacttc atgattaatt atgagacagg aatgcttgaa tggattgatg gtggagaggt
     1021 taagacaaga gatgatgctt acggtaatcc aatgattcgt tatggcactc gtgattatta
     1081 tctgaagtat gtcatctggt ttttgcatca tgaagttcag gcaactaaaa agattatctt
     1141 cagggatggt aataaaagaa attgccaccc aaacaattta ctcctggaga tttaatgtta
     1201 tgtattaaat tcgaatattt gacagataaa atgattaagc atgtatcaga cctgctcatc
     1261 aaagaaggtg gtttcggtga tgcttgcaat ccaaaagata tcttcattca tgccaccagc
     1321 ccaaacgcaa cactaaagac agcagttaca gcggaatggt ttgaacgaaa taaagcggaa
     1381 ttaggttatt ggtagtccga gactttaaga gaaattagaa aaacagctta tcagacgctc
     1441 tcaatcgcat tctaacgcaa aaaactatct tattttaggg tgggtaatac cactgtacag
     1501 gctacccctg aaaaagactg tttagctcgt tacagagcga ttgaggaggt ctggtaaaaa
     1561 ggagatttta aaaatgaacg aatatgaatt acagattttc attgatagcg acactgctat
     1621 gatggtgcag gctttcgctg attccggtgt atcaatt
//
LOCUS       DAEEKQ010000360         1626 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.369, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000360 DAEEKQ010000000
VERSION     DAEEKQ010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.369"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            87..851
                     /gene="nfeF"
                     /locus_tag="KLK57_004442"
     CDS             87..851
                     /gene="nfeF"
                     /locus_tag="KLK57_004442"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HBE0894611.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            complement(905..980)
                     /locus_tag="KLK57_004443"
     tRNA            complement(905..980)
                     /locus_tag="KLK57_004443"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(945..947),aa:Ile,seq:cat)
     gene            1105..1611
                     /gene="mug"
                     /locus_tag="KLK57_004444"
     CDS             1105..1611
                     /gene="mug"
                     /locus_tag="KLK57_004444"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HBE0894612.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
BASE COUNT          389 a          400 c          446 g          391 t
ORIGIN      
        1 tcgcttgcat ttatcatgat taacaatcat tatcatttac gaaaattatt tagatatatc
       61 tgattaccat cacgaaggcg ataacaatga ataactcccc ccgctacccg cagcgcgttc
      121 gcaatgatct gcgcttccgt gaactgactg tgttacgcgt tgagcgcatc agcgccggtt
      181 ttcagcgcat tgtcctcggc ggcgaggcgc tggacggttt tacatcgcgc ggctttgacg
      241 atcacagcaa actcttcttt cctcaacctg acgctcactt tgtgccgcca acggtaacgg
      301 aagagggcat cgtctggccg gaaggaccac gcccaccgtc gcgtgactat acgccgctgt
      361 atgacgaact acgccatgaa ctggcgatcg atttctttat tcacgacggt ggggtcgcca
      421 gcggctgggc gatgcaggcg caaccgggcg ataaacttac ggttgcaggt ccgcgcggtt
      481 cgctggtggt gccggaagat tacgcgtatc agctgtatgt ctgcgatgaa tccggaatgc
      541 ccgcactgcg ccgccgcttg gaaacgttga gcaaacttgc cgttaaaccg caagttagtg
      601 cgctggttag cgtgcgggat aacgcctgtc aggattatct cgcgcacctt gatggtttta
      661 atatcgaatg gctggcacat gatgagcagg cggtagatgc gcgtctggcg cagatgcaaa
      721 tccctgccga tgattacttc atctggataa ccggcgaagg taaagtcgtt aagaatttaa
      781 gccgccgctt tgaagcggaa cagtatgacc cacagcgggt acgtgcagcg gcttactggc
      841 acgcaaagta actgatatat aaaaagaaaa aggctgacga tttctcgtca gcctttgcta
      901 tatctggtgg cccctgctgg acttgaacca gcgaccaagc gattatgagt cgcctgctct
      961 aaccactgag ctaaggggcc atggtagcgg attataaagt aactccgtgt cgcaatccag
     1021 ccattaccgt gcgcctgctg tttttataaa caatgtattt tcaatccgtt atacttttcc
     1081 tggtgatgtt agaaaggagt aaatatggtt gaggatattt tggctccagg gttacgggtc
     1141 gtattttgcg gtatcaaccc tgggctttca tccgccggga ctggttttcc ctttgctcat
     1201 ccagcaaatc gcttctggaa ggtgatatat caggccgggt ttaccgaccg tcagttgaag
     1261 ccgcaggagg cacagcatct gctggattat cgttgtggcg tcaccaaact ggtagaccgt
     1321 ccaacggtgc aagccaatga agtttcaaag caggagctgc acgcaggtgg gcgtaagctg
     1381 attgaaaaaa ttgaagatta tcagccgcag gcgttggcga ttctgggcaa acaagcatat
     1441 gaacagggat tcagccagcg cggtgcacag tgggggaaac aaacgctcac cattggttcg
     1501 acgcagattt gggtgctgcc aaatcccagc ggtttaagtc gcgtttcact agagaaactg
     1561 gttgaagcgt atcgcgagct ggaccaggcg ctggtagtgc gtgggcgata aaaaacgcca
     1621 ccgaaa
//
LOCUS       DAEEKQ010000361         1617 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.370, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000361 DAEEKQ010000000
VERSION     DAEEKQ010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1617
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.370"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1199)
                     /locus_tag="KLK57_004445"
     CDS             complement(<1..1199)
                     /locus_tag="KLK57_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE0894613.1"
                     /translation="MKPAHNPSFFRSFCGLGCISRLSVEEQNITDYHRIWDNWAKEGA
                     ATEDRTQAVRLLKICLAFQEPALNLSLLRLRSLPYLPPHIQELNISSNELRSLPELPP
                     SLTVLKASDNRLSRLPALPPHLVALDVSLNRVLTCLPSLPSSLQSLSALLNSLETLPD
                     LPPALQKLSVGNNQLTALPELPCELQELSAFDNRLQELPPLPQNLRLLNVGENQLHRL
                     PELPQRLQSLYIPNNQLNTLPDSIMNLHIYADVNIYNNPLSTRTLQALQRLTSSPDYH
                     GPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIWHAFEHEEHANTFSAFLDR
                     LSDTVSARNTSGFREQVAAWLEKLSASAELRQQSFAVAADATESCEDRVALTWNNLRK
                     TLLVHQAS"
     gene            1291..1377
                     /locus_tag="KLK57_004446"
                     /pseudo
     CDS             1291..1377
                     /locus_tag="KLK57_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          428 a          302 c          449 g          438 t
ORIGIN      
        1 gatgcctgat ggaccaggag ggttttccgg agattgttcc atgtgagcgc gacacggtcc
       61 tcacagctct cagtggcatc agcagcaaca gcgaaagact gctgtcgaag ctccgcagag
      121 gcactgagtt tttccagcca tgcagcgacc tgttcacgga atccggaggt attgcgtgca
      181 gagacggtat cggaaaggcg gtcaaggaac gcggaaaagg tgttggcgtg ctcttcatgt
      241 tcaaaagcat gccatatctg tgatacatca gattgtttgt tttccgggaa ccatgctgtc
      301 acggcatcag ccagggggcg atggagtgta ttctgttgtc cgtcactcat ggagaagtaa
      361 atccgtgggc cgtggtagtc cggcgaagag gttaatcttt gcagggcttg cagagtgcga
      421 gtcgacaatg gattgttata aatattaaca tctgcataaa tgtgcagatt catgatactg
      481 tctggcaatg tgttcagctg attgttaggg atatatagtg attgcagacg ttgtggaagt
      541 tcgggcagtc tgtgtagttg gttttcccca acgtttaaaa gcctcagatt ttgaggaagg
      601 ggcggtagct cttgtaatct gttatcaaaa gcacttagtt cctgtagttc acatggtaat
      661 tctggtaagg cagtaagctg gttgttgcca acagaaagtt tttgtagagc cgggggaaga
      721 tcaggtagcg tctccaggct attgagaagg gctgagagtg actgcaagga agatggaaga
      781 gaaggcaaac atgttaaaac tctgttaagt gaaacatcaa gagcgaccag gtgaggcgga
      841 agagccggga gcctgctcag tctgttatcg ctggctttaa gtacagttaa ggacggaggg
      901 agttctggca gagagcgtag ctcattgcta gagatgttaa gttcttgtat gtgcgggggc
      961 aggtatggga gagagcgtaa tctgagtaaa cttaaattga gggctggctc ttgaaaagcc
     1021 agacatattt tcagtaatcg aactgcctgt gttcggtctt ctgttgcagc accttccttg
     1081 gcccagttat cccagatgcg gtgataatcc gtgatatttt gctcttctac ggataaacgg
     1141 gatatacatc ctaaaccaca aaaggagcgg aaaaaagaag gattgtgggc aggtttcatt
     1201 tattattgat cctcattgtt tatatattaa tatgttcgtg caaatggagt gaggattctt
     1261 taaaaaaacg cgcctgtaaa tagttaagaa caggaagaac tgttggggat gtggctggcc
     1321 gaaaatgaag gggtgaagtt ctggctcaat gtgctgactg aactgaaaaa acgcggttgg
     1381 tcgccctacc ttttcatcaa gccagtagtc tgcccaccac tgcatcattt ctctgcgctt
     1441 atcgagatac tgagcatggt tgtaaattca gctaacccag tgcgttgaca aaaaattagc
     1501 gcaagaggac aaaaaatcac cttgcgctaa tgctctgtat caggtcacta ataccatcta
     1561 agtggttgat tcatagtgac tggatatgtt gtgttttgca gtattatgca gtctatt
//
LOCUS       DAEEKQ010000362         1574 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.371, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000362 DAEEKQ010000000
VERSION     DAEEKQ010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.371"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            282..686
                     /gene="stpA"
                     /locus_tag="KLK57_004447"
     CDS             282..686
                     /gene="stpA"
                     /locus_tag="KLK57_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HBE0894614.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            complement(733..1257)
                     /gene="ygaP"
                     /locus_tag="KLK57_004448"
     CDS             complement(733..1257)
                     /gene="ygaP"
                     /locus_tag="KLK57_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HBE0894615.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(1267..1566)
                     /locus_tag="KLK57_004449"
     CDS             complement(1267..1566)
                     /locus_tag="KLK57_004449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HBE0894616.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
BASE COUNT          426 a          360 c          363 g          425 t
ORIGIN      
        1 tcttttattc tgttatattg catttttatt cacttcggtt ttgtcaggct tgcggaatta
       61 gcgagcagag agcgccgttc tgttcaccaa taatctaaat tactcatcaa aatggctaat
      121 atataatctt gaaattattc tgagagtttc agaaaataac agctgaaata atctcgcgca
      181 agactgtaaa tagattaaat tttgtggaaa tataataagt gatcgcttac actacgcgac
      241 gaaatacttt tttgttttgg cgttaaaagg ttttctttat tatgtccgta atgttacaaa
      301 gtttaaataa cattcgcacc ctccgtgcga tggctcgcga attctccatt gacgttcttg
      361 aagaaatgct cgaaaaattc agggttgtca ctaaagaaag acgtgaagaa gaagaacagc
      421 agcagcgtga actggctgag cgtcaggaaa aaattagcac ctggctggag ctgatgaagg
      481 ctgacggaat taacccggaa gagttattgg gtaatagctc tgctgctgca ccacgcgctg
      541 gtaaaaaacg ccagccgcgt ccggcgaaat ataaattcac cgatgttaac ggtgaaacta
      601 aaacctggac cggtcagggc cgtacaccga agccaattgc tcaggcgctg gcagaaggta
      661 aatctctcga cgatttcctg atctaattac tgaggccgga tgtcgctgct agggcgcgtc
      721 cggcgtcgct tctcaagctc gttggttcca cggcatctta tctaacaacc ttgccattcc
      781 acaaaaaccg ctgattcctg caaacagtaa cccggcacca acaaagccac ttaataagaa
      841 gaaaccgcta tttacggtat aacccagtac aacgccgatt aatattaaac cacccgcagc
      901 gatctgcacc tggcgcatta acggcaaggg ttgagattta tttaccgcta ctggcaatcc
      961 agctcttttc cagccatcaa tcccatcttc gagtaaaaat atttctgcgg gggcggcaat
     1021 cgctgctaat ttatcggcgt tattactggt gcgtttacct gcctggcagt ggaatataat
     1081 ttgctcgcga cgtaatttag gtggaagacc tgactgttcc agcacggata atggagccag
     1141 atctgcttca ggaatatgtt cacgaagata ttcatcagcg tcacgaatat cgattaactt
     1201 tgcgccgcgt gcgattaatt cttgtgcatc atgcggcgaa atggttgtca aagccatagt
     1261 gactccttac ggacaataga catttttcag ggtggcgata atggcattta ccgcctcatt
     1321 tttaatggaa tatagaatgc gttgggcatc ccgttggctg tcgataagcc cttcgtcccg
     1381 catacgagcg agatgctgtg atgtcgcaga ggcactcagt ccggtaatgc gcgtcagctc
     1441 tcccgcgctg gtgccggggg aaccgctaag catgcacaga atcagcaatc gtttaggatg
     1501 gctcattgct ttcaataagg ccgctgcctg ttcggcactg gcctgtaatt gcgcgagttc
     1561 agtcatattt attt
//
LOCUS       DAEEKQ010000363         1564 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.372, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000363 DAEEKQ010000000
VERSION     DAEEKQ010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.372"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            502..957
                     /gene="csgB"
                     /locus_tag="KLK57_004450"
     CDS             502..957
                     /gene="csgB"
                     /locus_tag="KLK57_004450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HBE0894617.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSTQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
     gene            998..1450
                     /gene="csgA"
                     /locus_tag="KLK57_004451"
     CDS             998..1450
                     /gene="csgA"
                     /locus_tag="KLK57_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HBE0894618.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGNHGGGGNNSGPNSEL
                     NIYQYGGGNSALVLQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSATL
                     DQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
BASE COUNT          474 a          302 c          357 g          431 t
ORIGIN      
        1 aatgatagaa aagttgtaca tttggttttt attgcacaat tttaaaaaat catacaaatg
       61 gtgataactt actaataatg catataaaaa atatttcggt gtagtccttt cgtcatgtaa
      121 aaagttgttg ttttttctcc acacctccgt ggacaaattt ttactccaaa aagacgaggt
      181 ttgtcacggc ttgtgcgcaa gacatatcgc agcaatcagc gatgggcaag aagaatgact
      241 gtctggtgat ttttgatagc ggaaacggag atttaaaaga aaacaaaata ttttttgcgt
      301 agataacagc gtatttacgt ggcttttaat actttggtat gaacaaaaaa agaaaaatac
      361 aacgcgcggg tgagttatta aaaatatttc cgcagacata ctttccatcg taacgcagcg
      421 ttaacaaaat acaggttgcg ttaacaacca agttgaaatg atttaatttc ttaaatgtac
      481 gaccaggtcc agggtgacaa catgaaaaac aaattgttat ttatgatgtt aacaatactg
      541 ggtgcgcctg ggattgcagc cgcagcaggt tatgatttag ctaattcaga atataacttc
      601 gcggtaaatg aattgagtaa gtcttcattt aatcaggcag ccataattgg tcaagctggg
      661 actaataata gtactcagtt acggcaggga ggctcaaaac ttttggcggt tgttgcgcaa
      721 gaaggtagta gcaaccgggc aaagattgac cagacaggag attataacct tgcatatatt
      781 gatcaggcgg gcagtgccaa cgatgccagt atttcgcaag gtgcttatgg taatactgcg
      841 atgattatcc agaaaggttc tggtaataaa gcaaatatta cacagtatgg tactcaaaaa
      901 acggcaattg tagtgcagag acagtcgcaa atggctattc gcgtgacaca acgttaattt
      961 ccattcgact tttaaatcaa tccgatgggg gttttacatg aaacttttaa aagtagcagc
     1021 aattgcagca atcgtattct ccggtagcgc tctggcaggt gttgttcctc agtacggcgg
     1081 cggtaaccac ggtggtggcg gtaataatag cggcccaaat tctgagctga acatttacca
     1141 gtacggtggc ggtaactctg cacttgttct gcaaactgat gcccgtaact ctgacttgac
     1201 tattacccag catggcggcg gtaatggtgc agatgttggt cagggctcgg atgacagctc
     1261 aatcgatctg acccaacgtg gcttcggtaa cagcgctact cttgatcagt ggaacggcaa
     1321 aaattctgaa atgacggtta aacagttcgg tggtggcaac ggtgctgcag ttgaccagac
     1381 tgcatctaac tcctccgtca acgtgactca ggttggcttt ggtaacaacg cgaccgctca
     1441 tcagtactaa tacatcattt gtattgtcag cgccagtgat ataagacggt aattcgccat
     1501 ttggattgtc cgctccaccc aacatgttgt ttccttaagg ttctcacacc agaaaggaca
     1561 tcaa
//
LOCUS       DAEEKQ010000364         1558 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.373, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000364 DAEEKQ010000000
VERSION     DAEEKQ010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1558
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.373"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            387..1469
                     /locus_tag="KLK57_004452"
     CDS             387..1469
                     /locus_tag="KLK57_004452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HBE0894619.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYARLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNNQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLVEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGMVYQF"
BASE COUNT          487 a          317 c          315 g          439 t
ORIGIN      
        1 ttttttacgt acgtaaacca ctaaatgata tttgcgagaa tgctaccgag tattgaaaac
       61 accactacaa atacataagc aaatcccaac aaataaccaa caaataattt ccagtgttat
      121 ttttagccgg tttaaattga accttcaaat tatagagcac ttataaataa cagccgttaa
      181 tataaattgg ctaatagatt tatttttatt cagtcaagag ccatgaatag gattcgatag
      241 aaaaaagttc agataaaaat agagatctac ttcacaaatc aaacgagaaa ccaaaactta
      301 catcttgaaa taatcacatt gattagatga atatttatcg cgcaatgaca tcatttttta
      361 ataatagttc aaaaaaaggg ctcacgatga aaaaattaac agtggcaatt tctgctgtag
      421 ctgcatcagt actgatggcg atgtctgctc aggcagctga aatttataat aaagacagta
      481 acaagctgga tctgtacggg aaagttaatg ctaagcacta cttctcctct aatgatgcag
      541 atgatggtga tactacttat gcccgtcttg gcttcaaagg tgaaacccaa atcaacgatc
      601 aactgactgg tttcggtcag tgggaatatg aattcaaagg caaccgcgct gaatctcaag
      661 gttcctccaa agataaaacc cgtcttgcct tcgctggcct gaaattcggt gactacggct
      721 ccatcgatta cggccgtaac tacggtgtag catacgacat cggtgcgtgg actgacgtcc
      781 tgccagaatt cggtggtgac acttggactc aaacagatgt gttcatgact ggtcgcacca
      841 ctggtgttgc aacttatcgt aacaacgact tctttggtct ggttgatggt ctgaactttg
      901 ctgctcagta tcagggtaaa aatgaccgca ctgacgtaac tgaagctaat ggtgatggtt
      961 tcggtttctc cactacttat gagtatgaag gattcggtgt aggtgcaacc tatgctaaat
     1021 ctgaccgcac taataatcag gttatctacg gtaacaacag cctgaatgca tctggtcaaa
     1081 atgctgaagt atgggcagct ggtctgaaat atgatgcgaa caacatctat ctggctacca
     1141 cctattctga aacccagaac atgactgttt ttggtaataa ccatattgcc aacaaagcac
     1201 aaaacttcga agtagttgca caatatcagt tcgacttcgg tctgcgtccg tccgttgctt
     1261 acctgcaatc taaaggaaaa gacttgggtg cgtggggtga tcaggacctg gttgaatata
     1321 ttgatgtagg tgcaacctat tacttcaaca aaaatatgtc cacttttgtt gattacaaaa
     1381 tcaacctgat tgataagagc gatttcacga aagcatctgg cgttgctacc gatgatatcg
     1441 ttgctgtagg tatggtttac cagttctaat ttgattacta aaagatatgt tgcgggaggc
     1501 tttgcctccc caacatataa gtggctccct caagccactt cctttaggag cacaacct
//
LOCUS       DAEEKQ010000365         1547 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.374, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000365 DAEEKQ010000000
VERSION     DAEEKQ010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1547)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1547)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1547
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.374"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            241..732
                     /locus_tag="KLK57_004453"
     CDS             241..732
                     /locus_tag="KLK57_004453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBE0894620.1"
                     /translation="MSTETIEIFNNSDEWANQLKHALSKGENLALLHGLTPDILDRIY
                     AYAFDYHEKGNITDAEIYYKFLCIYAFENHEYLKDFASVCQPKKKYQQAYDLYKLSYN
                     YSPYDDYSVIYRMGQCQIGAKNIDNAMQCFYHIINNCEDDSVKSKAQAYIELLNDNSE
                     DNG"
     gene            1067..1525
                     /locus_tag="KLK57_004454"
     CDS             1067..1525
                     /locus_tag="KLK57_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001426852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HBE0894621.1"
                     /translation="MDLENKFSYHFLEGLTLTEDGILTQGNEQVYIPQKELGVLIVLL
                     ESAGHVVLKDMIIESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYRFS
                     GQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDG"
BASE COUNT          582 a          234 c          249 g          482 t
ORIGIN      
        1 aggtcataca cattaaggca tttatgtaat gtcgtaatta atataactaa taaggtgaat
       61 attagtaaaa caactaatga tgttattagt ttgtagtgaa cagtactttt accaataatg
      121 aaaaatatac cataagcaat gcaagcttaa aactaataat ttaaaaaaga taaatgtaaa
      181 aatattaatg agtcaatcaa taataattta gcatcacgag acacatcaca gaggaatatt
      241 atgagcacag aaacaattga aatattcaat aatagtgatg aatgggcaaa tcaactaaaa
      301 cacgcattat cgaaaggaga aaatctggca ttactacatg gtttaactcc tgatatcctt
      361 gatagaatat atgcatatgc attcgactac catgaaaagg gtaatataac ggacgcagaa
      421 atttattata aatttttgtg catttatgcg ttcgaaaatc atgagtatct aaaagatttt
      481 gcatcagtat gtcagccaaa aaagaaatat caacaagcat atgaccttta caaactaagt
      541 tacaattact ccccgtatga tgactattca gttatttatc gtatgggtca atgtcagatt
      601 ggggctaaaa atatcgataa cgcaatgcaa tgtttctatc acattattaa caattgtgag
      661 gatgatagtg ttaagagtaa agcgcaggca tatattgaac tcttaaacga taattcagaa
      721 gataatggct aagcattaca atcatttggt taaatttgct ggttatagtg cagttaacta
      781 ctataaccag cacctcttat gttttcttaa atcagcatct taaaacagtt aagagcatac
      841 tataaatcat tatttttcaa tgcgttagag aacaatctac tttataaaaa ttgaataagg
      901 ctatttcaga ttacatcact gaattcctaa gataaattag tgaacacaac catagatatg
      961 tcgaatgtat tttcctgtag cattaatgac acataaatat gaatagccat aatttctatt
     1021 gcttaagcag gatgcaagaa accaattttt tcatagaggt taactaatgg acttagaaaa
     1081 taaattctca tatcattttc ttgagggatt aacgctcacg gaagatggaa ttcttactca
     1141 aggaaatgag caagtttata ttccacagaa agagttaggt gtattaatag tattacttga
     1201 atccgctggt catgttgtac tgaaagatat gatcatcgaa tcagtatgga aaaatattat
     1261 tgttagtgac gagtccctga caagatgtat ctattctttg cgctgcattt ttgaaaaaat
     1321 tggctatgat cgttgcatag aaacaatcta ccggaaaggt tatcgtttca gcgggcaggt
     1381 tttcaaaact aaaattaatg aagataatac ttcagactat tccatagcta tattcccttt
     1441 cactacttca ttgaatacac tggatccatt aatacttaat caggaattag tgcaaatcat
     1501 ttcaaataaa aaaatcgatg gttagactgg ccccctgaat ctccaga
//
LOCUS       DAEEKQ010000366         1533 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.375, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000366 DAEEKQ010000000
VERSION     DAEEKQ010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1533
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.375"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            97..759
                     /locus_tag="KLK57_004455"
     CDS             97..759
                     /locus_tag="KLK57_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBE0894622.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPFLLFKNLHREFIFSLDGAATEHD
                     TLLPRFTDDISRQSWVSEKVFCNPPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNRLTIRSPFPSAVVVFKKEPRKHEITQMVCCAD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKYIADALRMPLSTSYRIAQR
                     LS"
     gene            1123..1533
                     /locus_tag="KLK57_004456"
                     /pseudo
     CDS             1123..1533
                     /locus_tag="KLK57_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170527.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          478 a          307 c          302 g          446 t
ORIGIN      
        1 gtttagaaaa cctgttaagc atctcttaaa agtaagcatc tcttaaaaga gagtaaacct
       61 tgtcaagagt gattgcaata aaaattttta tttcttatgt caattgagca tattttaatc
      121 gggaagcatc atgggggttc tgattactgg agaactccat ttttgttatt taagaactta
      181 caccgtgaat ttattttcag tctagatggt gctgcaactg agcatgacac gctgttacca
      241 cgattcaccg atgatataag ccgtcaatca tgggttagtg aaaaggtctt ctgtaacccg
      301 ccgtacagcg atattccgtc gttcctctta aaagcgtctg aagcggattt agtagctttc
      361 ctgataccgc atagagccaa caccagctac tggcttaggc acatttacag caataaccac
      421 tgtcatgaaa tccgtattct tcatcgtgca gttaaatacc ttccaccagc agggcataac
      481 aggcttacta ttaggtcacc gtttccaagt gcagtcgttg tttttaaaaa agaaccacgt
      541 aagcatgaaa ttacacagat ggtttgctgt gctgatacat tgctaccgtt aaccatcatt
      601 aaccgtggtg gactaagagg aagacccacc atttacccac ctgagacgct cgacagcttc
      661 ataaaactat acaggcaagg aaaacccatt aagtacattg cagatgctct cagaatgcct
      721 ctgagcacgt catacaggat tgctcaaagg ttgagctaaa attctcataa atatggtcat
      781 ttacaagaca ggtgattcag atgttaagaa gtatccctgt gaacaataaa tgaccaatca
      841 gtaatcattc tgtgaaataa ttgttgcaat ttataatcgc atctgttata gtttctatat
      901 gggctgataa aaaagttcat gtcttgaagt ggtaacactt ggggaggtgt cagatggttg
      961 gcgtatatta acctctcatt cagggtatac ctcagcgttt taatcatcgt actgtttcag
     1021 gcatggactt ctttatcagt ccatcaatcc cccagatatt ttaatcacac aggctaatcg
     1081 gttttatccg gttggccttt tcacgtatga ggtaatttat gaatgtctaa atataaccca
     1141 aatcagtttt ataaatttga tgtgcgaatc tctcaacttt cagaagaaat taacgcagca
     1201 gcaagattga tttatatggt catggtccat agctgtgact tctatgaaga gaaatacaaa
     1261 tcatattcac caagtattga ccatctcgct tatatggctg gtgtatcaaa gagaacagca
     1321 atcagagcca ttgagacact tgagaaacac ggtttaattc aggtgcaaaa gaatcagggg
     1381 cgtcaaagca cttatcgtgt attcacactg gatgataagc cggaattact tgtaagacct
     1441 acatcacagg aagttgcagc ctcagtaaga caaaatgaca ccactaccag tgacaaaatg
     1501 tcatgggggt agtgacaaaa tgtcacccgt gtt
//
LOCUS       DAEEKQ010000367         1525 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.376, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000367 DAEEKQ010000000
VERSION     DAEEKQ010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1525)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.376"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            125..1168
                     /locus_tag="KLK57_004457"
     CDS             125..1168
                     /locus_tag="KLK57_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HBE0894623.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNASLKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
BASE COUNT          426 a          347 c          331 g          421 t
ORIGIN      
        1 ctcaaggaaa gcatgaaggg gcttcgtaag caatattcat tcggtgccaa gacaggactt
       61 gatacacaga atctcaactt ttatgctctc agactatatc accacatgtt ttacaggctc
      121 agaaatgaac gatagattat gctttgaagt gcacgataac aagggttatt tcgtatttcc
      181 ggatacctgg tttggcccat tgctggggga gtttgaagaa gtgcttgatg catacgacgc
      241 tgatgaaatc agcgagacga gttacatcaa caaactccga cgccttgctc aacgggagcc
      301 ggactttatt gatatacacg ctcatctggc atatgcattt ctggaacaga atgccccgcg
      361 taaggcactg aacgcctctc ttaaaggact ggcggcaggt aatcgtatca tcccggaaag
      421 tttctgtggt gaaataatct ggatgcatcc cgaaaaccgg ccttatctgc gcgcgcttta
      481 tgcagcaatt ctcgcaaatg tgcacttaca gcgccatcag gatgcggtta tgctcactga
      541 taaaatactg gcatacaatc ctgaagataa tcagggggct cgctggttgt tgggttcaga
      601 gctgttacgc accggcgatc atgagcgagc cttcagtgta ctgaaagagc atgctgatga
      661 attttcacct tactggtatg aactcggtct cctgcatttt ctgaacgggg aacatgttaa
      721 ggccgccaca gcttttcgcc atggctttgc cacaaacact tacatcgcag aaatgctgtg
      781 cggaaatctg catccatttc cacttgctgt gtggcatgac ttctccggct ctctggatac
      841 ggcagaagat tactatgcaa catactcacc attatgggga caatatccgg aagcattgct
      901 gtttgtaaac tggctctata accattcctc cgtgctgcat gaacgatcag aaatcattaa
      961 atgcgccgaa atgctgattc aggaagatga tttcgaaata tgcgaaagta ttttaagaca
     1021 gcaggagcac ctgtggaaaa gaattgacaa gacactttct gaggaaattg tacagaaatg
     1081 cagaaatatg aacggtgaat atatctggcc ctggatattg cccttttccg cagcagggat
     1141 gaaacattca agcatccagt atcagtagat attgcattag tgtaacttta aaacaagtaa
     1201 taatttatta tccaataata acactgtgtt ccttccccag gtgattaccc tggggttatt
     1261 ttctgaaagt ttcctttgca aaaggatagc gttgtaaatt actttaacga attactccat
     1321 gaactcatta aagttctttc gtaatacttt atcgtcaaac acgttatagc tggcatcaaa
     1381 aaccacagat ttaccctgcc agcgggtaat cccctcctct ggcattttcg gttgaactgg
     1441 tatccccgtg gatttgcccc tatatttcca gacatctgtt atcacttaac ccattacaag
     1501 cccgctgccg cagatattcc cgtgg
//
LOCUS       DAEEKQ010000368         1462 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.377, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000368 DAEEKQ010000000
VERSION     DAEEKQ010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1462)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1462)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1462
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.377"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            118..474
                     /locus_tag="KLK57_004458"
     CDS             118..474
                     /locus_tag="KLK57_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HBE0894624.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            574..>1462
                     /locus_tag="KLK57_004459"
     CDS             574..>1462
                     /locus_tag="KLK57_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0894625.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHP"
BASE COUNT          353 a          346 c          296 g          467 t
ORIGIN      
        1 cctcgcccct cactctttcc aataatgttg cacaagatac tgtgactctc ttcaagccgt
       61 cagcaggctg ataatgttat ttatatttta ttgtttattt catgcagcca ttacacgatg
      121 aacgcttatg aactccaggc actccgccat atttttgcca tgactattga tgaatgcgcg
      181 acctggattg cccagaccgg tgacagtgaa agctggcgtc agtgggaaaa tggcaaatgc
      241 gccattcctg attgtgtagt cgagcaactg ttggctatgc gtcagcaacg aaaaaaacat
      301 cttcatgcca tcatagagaa aataaataac cgcatcggca acaacaccat gcgttttttc
      361 cccgacttaa ccgcgtttca acaagtctac cctgatggca attttattga ctggaagatc
      421 tatcaatcgg tagccgctga attatatgca cacgatctgg agcggctttg ttaatttttc
      481 cacagaaagg aattgtcgtt gttacaacaa taatgaatgg atactgacac aacatcgcta
      541 cactttttaa agcacctttg ctaagtagaa cctatgaaaa atccctatta ccctaccgca
      601 cttgggttgt attttaatta cctggtgcat ggtatgggcg tcattttgat gagcctgaat
      661 atggcctcgc tggagacact ttggcagact aatgccgcgg gtgtctcgat agttatctca
      721 tcgctgggca ttggtcgatt aagtgtcttg ctttttgcag gattattatc cgatcgcttt
      781 ggtcgccgcc cttttatcat gctcgggatg tgctgctata tggccttctt ttttggcatc
      841 ctgcagacca ataacatcat tatcgcttat gtttttggct ttctggcggg aatggcaaac
      901 agttttctcg atgcaggcac ttatcccagt ttgatggaag cttttccacg ctcacctggg
      961 acagccaata ttttaattaa agcatttgtt tccagcggac aatttttatt accgctaatc
     1021 attagcctgt tagtgtgggc tgaactgtgg ttcggttggt cctttatgat tgctgcaggc
     1081 attatgttta ttaacgctct gtttttatac cgttgtacgt tcccacccca tccgggtcgt
     1141 cgcttacctg tcataaagaa aaccaccagc tctacggaac atcgctgttc aattatcgat
     1201 ttagccagtt ataccttata tggctatatc tcaatggcaa cgttttatct ggttagccag
     1261 tggctggcac agtacggaca atttgttgca ggcatgtcat acactatgtc gatcaaacta
     1321 ctcagtatct acaccgtggg ttcgctgctt tgtgtattta ttaccgctcc actcattcgt
     1381 aataccgttc gcccaacaac attactgatg ctgtacacct ttatctcatt tattgctctg
     1441 tttaccgtct gcctgcatcc ca
//
LOCUS       DAEEKQ010000369         1457 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.378, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000369 DAEEKQ010000000
VERSION     DAEEKQ010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1457)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1457)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1457
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.378"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="KLK57_004460"
     CDS             complement(<1..1010)
                     /gene="cadA"
                     /locus_tag="KLK57_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBE0894626.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYT"
     gene            complement(1090..1437)
                     /locus_tag="KLK57_004461"
     CDS             complement(1090..1437)
                     /locus_tag="KLK57_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium-binding protein"
                     /protein_id="HBE0894627.1"
                     /translation="MTALMILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDL
                     IRFEGVNIRNFVSLICSVLGCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQ
                     SHSMDNHTASNAH"
BASE COUNT          406 a          320 c          364 g          367 t
ORIGIN      
        1 gtgtaaggca cccacgcgga gtcaaagtgg atggatttca catccagtgt tttcttgatg
       61 aagtcggtgt tgtacagcag accatcatag gtagagttgg taattacagc atgtaccggc
      121 caggttgcgt ttggtgtttc tttcacgcgc ttagcaatgg tagcgtgctg gaattcactc
      181 tgtgggatac caccaagaat accgtaagcg ttacgggtcg ggcggaaata gattggcgta
      241 acatcgctca tcatcatcag gtgggtcagc gatttgtggc agttacggtc aatcagaatg
      301 gtgctgcctg ccggagcaga gtacatgcca acaattttgt tcgcagtgga agtaccgttg
      361 gtcaccatgt agctgcggtc tgcgttaaag acgcgagcga tatactgttc tgcttctttg
      421 tgtggaccac tatgatccag cagagaaccc agttcagata ctgaaatgga aatatcagat
      481 ttcatggtat tcggaccaaa gaaatcatag aacaggctac ctaccgggct tttctggaat
      541 gcagtaccgc ccatgtgacc aggagtacag aaagtatatt taccttcacg aacatattta
      601 aacagtgctt tagtcagcgg aggcagaata gtgttgatat attcgtcagt ggtctgcttg
      661 atcttattag caatatcttc agcagcaccc agcgcatatt caaagaagct aatctgtaaa
      721 cgcaggtcat tcaggcttac atcgagagtg gaatacgtat tagcgaacgc gtacaacggc
      781 aggttctcgt tcattttgct aatttcttcg cacagctcga gattatattt atcccagtca
      841 aaaataacgc cgcacagacg cgcattgttt tcgatcagtt ttaataagtc gtcacggtcg
      901 ttcgggtaaa caatctggaa gttcagacgt tcaagcgcgc gatgaagttc acggatgggt
      961 tcttctttaa aataaacccc catgtgattc aatattgcaa taacgttcat agtcatatct
     1021 ccaggtaaaa aaggcccctc ccaacacatg ggacaaaatg aaaggaggag cctcggaaaa
     1081 tacttttaat taatgtgcgt tagacgcggt gtggttatcc attgagtggc tctggcgctc
     1141 gtgcattttg cgagcgtaga acatcaggat aatcaggctg acgatgaagg tacctgccag
     1201 ctcgaaggag cttgcgccca tcagcgcgat gaagcagaac acacaaccca gtacagagca
     1261 gatcaggctg acaaagttgc ggatgttaac gccttcaaaa cgaatcaggt caacgcaaga
     1321 gtagaagtac ggcagcatag tcagcagtac tgcgataccg gtcagttcac cgaacaggtc
     1381 agatgcttta ccaccggcag agttcatcag agtgatgagg atcatcaggg cagtcatttt
     1441 cactgcagcc agcagca
//
LOCUS       DAEEKQ010000370         1436 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.379, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000370 DAEEKQ010000000
VERSION     DAEEKQ010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1436
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.379"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..218)
                     /locus_tag="KLK57_004462"
     CDS             complement(<1..218)
                     /locus_tag="KLK57_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001462196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HBE0894628.1"
                     /translation="MKKFFRHFFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQKW
                     RETGPNGDATVIFRYATSTNNLVFYKPT"
     gene            complement(271..867)
                     /locus_tag="KLK57_004463"
     CDS             complement(271..867)
                     /locus_tag="KLK57_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0894629.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            complement(894..>1436)
                     /gene="yadL"
                     /locus_tag="KLK57_004464"
     CDS             complement(894..>1436)
                     /gene="yadL"
                     /locus_tag="KLK57_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HBE0894630.1"
                     /translation="FSVPSYAATDSIGLTVITTVEIGTCTATLVNDSDQDISVVDFGD
                     VYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRATCEGTANDGAGLANGSTAAD
                     KASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNAANAVVYYPMSARLVVEKNKT
                     VSNVTAGKFSAPATFTVTYN"
BASE COUNT          387 a          314 c          297 g          438 t
ORIGIN      
        1 tgcgtcggtt tgtagaaaac taaattattt gtacttgttg catatctgaa tatgacagta
       61 gcatcgccat tcggccccgt ttctctccat ttttgatctg acggaccaac gccgtaacta
      121 ttcccaacaa tatatcctac gttatcatca gtgccagcac tcgcggtata acaactcaag
      181 cacagaatta aaaaaaaatg tctgaagaat ttcttcatta tcactccacc atctactgct
      241 gtaacagtcg cagttatttg ttttgatcta ttattcgtag tacacatcaa ctaatatatg
      301 actcattact tggccagata tgacatccag cgcgggatcg attttttgca ttctggcata
      361 aaatttccat gaagaattat gcagggcaga agtcattact ggatacacgg aacgagaatt
      421 tcctgctgaa ctaataacat tgaatatatt ggtcgggtcc tgaatggaaa agagaacgac
      481 accgacattt ttagccccgg ttgcgacttc attggcaaat atttgccttg aacctgcagc
      541 catgacaccg ctaagggctc taaaatcaat atgtaactga ttaagcacat cgccagttcc
      601 ctgtaatgca cagtctgata ctgtgacagt gaactctttt ccacctggtt gatagctttc
      661 aggagtaaca ttatctgaaa aatatcccac cccaacgacg ccaagatcaa tattaccctg
      721 gttactgatt ccgcttttgc aagtgctgtt ttttacattg gcaacaaggt caatatcctg
      781 cccagcgatg gctggatagg taatgggaaa ctgtagtgac agaaacagaa ttattctctt
      841 catcagctta cgttgagtgg gatgcatata tagttcctgg atctgtcagt ctgttagtta
      901 taggttactg taaatgtggc aggtgcagaa aatttacccg cagtgacatt gcttacggtt
      961 ttattttttt cgacgaccag tcgagcactc atcggataat aaacaaccgc gttggctgca
     1021 ttggagatag tgacctcttg agatgctggt gtcacacaac taaattgtgt tgcgctccct
     1081 gttgccggag tttcggtgct ccagacttca acggcgacgg cacttgcttt atctgcggct
     1141 gtggatccat ttgcgagccc cgcaccgtca ttagcagtac cttcgcatgt ggctcgcttg
     1201 gttaatttta tttgtgcttt tttattgggg atacccgcac agtctttgaa tttgagtttg
     1261 aatgttttaa ctttggtctt ggcattgatt tcagaaatat atacatcgcc aaagtcaaca
     1321 acagaaatgt cctgatcaga gtcatttact aatgtagcgg tacaagtacc tatttctacg
     1381 gtagtaatca cggtcagccc aatgctgtct gttgccgcat aagagggtac gctgaa
//
LOCUS       DAEEKQ010000371         1430 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.380, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000371 DAEEKQ010000000
VERSION     DAEEKQ010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1430
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.380"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            13..438
                     /locus_tag="KLK57_004465"
     CDS             13..438
                     /locus_tag="KLK57_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000056357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBE0894631.1"
                     /translation="MLRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVK
                     TRIKLFWRPANIRLIPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNM
                     IRPFETLTLPLPANSAGGQMTWRFINDYGAVSDPIKMTL"
     gene            463..>1430
                     /locus_tag="KLK57_004466"
     CDS             463..>1430
                     /locus_tag="KLK57_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HBE0894632.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEIDHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIPQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDL"
BASE COUNT          386 a          331 c          341 g          372 t
ORIGIN      
        1 tcgcaagaaa aaatattacg cattattcac accaaaggtg tgtcattgcc tgatgatcgc
       61 gagtcagtct tctggctgaa tattaaaaat attccgccat cagcgagcaa caaagcgacg
      121 aactcgctgg aaatagcagt aaaaacgcgt atcaagctgt tctggcgacc agcaaatatc
      181 cgccttattc ccgaagatgc tgcgccgaaa gtgaaatggc gccgggaagg gcgaaatctg
      241 atagcagaga atcccaaccc aattcatatc agtgtgatgg atgtcatcgt tgatggccat
      301 gatgtgcccc tcaatatgat ccgtcctttt gaaacgctca cgttaccttt acctgctaat
      361 tctgcgggag gccaaatgac ctggcgattt attaatgatt acggcgcagt cagtgacccg
      421 ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac gaccagaata
      481 gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat ggccgaggtc
      541 agtgtattca atccggcgct tctggaaatc gaccatcaat ccggagtcga tattcgccag
      601 tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt tatcaacggt
      661 aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc tgatctgcac
      721 gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt tgatgcgctc
      781 aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg tattgaaggc
      841 tcatcctggc agtttgacag tgataaattg cagctgaata tatccattcc ccaaatctac
      901 atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg gattaacgcg
      961 ctcaccatca actacgattt ttctggttca catacactac gttcagatta tggttcacaa
     1021 gagacagata ccagttatct caatctgcgc aatggactga atattggacc gtggcggcta
     1081 cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc cattagtacc
     1141 tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga tacgtggacg
     1201 gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac tgataacgat
     1261 atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc aaagtccaac
     1321 gccaccgtca tcattcggca gaatggctac gtgatttatc agtctgccgt tccacaaggt
     1381 gcttttgaga tcaccgatct caacaccgcc agtacaggtg gcgatttgga
//
LOCUS       DAEEKQ010000372         1427 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.381, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000372 DAEEKQ010000000
VERSION     DAEEKQ010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1427)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1427)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1427
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.381"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..119)
                     /locus_tag="KLK57_004467"
     CDS             complement(<1..119)
                     /locus_tag="KLK57_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HBE0894633.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQH"
     gene            complement(131..823)
                     /gene="rutB"
                     /locus_tag="KLK57_004468"
     CDS             complement(131..823)
                     /gene="rutB"
                     /locus_tag="KLK57_004468"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HBE0894634.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPDDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            complement(823..>1427)
                     /locus_tag="KLK57_004469"
     CDS             complement(823..>1427)
                     /locus_tag="KLK57_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HBE0894635.1"
                     /translation="GDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCF
                     GKGVNTPTAFAPTAVRMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADE
                     EALSWLTEQSQKDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPG
                     AEGVLLTFDDFLSGIETFGERIQPLMQCRAHLPALTQEVA"
BASE COUNT          323 a          396 c          396 g          312 t
ORIGIN      
        1 ttatgttgat caaaagccag cgtaccggag acatacacca cgccatcagc cagcgtgccg
       61 ggaacgaagg gggccagcgg tgcgctgctg ccagcgggaa taattacgga ttttggcatc
      121 gttaaactcc ttaagcgata cgagcaaagg acgtgggaga aagcgcgtcg cagaacgttt
      181 cgacgtcgct gacccagcca aaaaaggttt cgatattgaa caacgcggct ttctgtgcaa
      241 attcaggccc cgcctggtgt gttgcgtctt ccagcaccac gccgaaatac tccagaaaaa
      301 agccatcgcg tagcgtcgat tcgacgcaga cgttggtagc gataccggtg aaaaccagat
      361 ggcgtattcc acggctgcgc aaaatgctgt ccagcggcgt attgaagaaa ccgctgtagc
      421 gcggcttcgg cagcacaata tcgtcaggct gcggcaccag ttcatccacc agttgataat
      481 cccaggagcc tttcgccagc aatttcccct gcagctgcgg ctgcttacgc atggttttca
      541 gggcgttcga tttatgaaaa ttcggtgagc cgggtccgcc agcttcgaca tactgttcat
      601 cccagccatt ttgaaaccag atgatcagca tccctgccgc tcgcgctgcg gtcacggcgg
      661 tttgaatgtt ggcaatgacc gggcgagtgg ttgagacatc aaacccggcg agatctaagt
      721 agccgcctgg cgtggcataa gcgttttgca tatccaccac gatcagcgca ctttgctgcg
      781 gatcgaaggt aatggcttcc ggtcgagcgg ttaaggtcgt catcatgcca cctcctgagt
      841 cagcgcaggg agatgggcgc ggcactgcat cagtggttga atgcgctcgc cgaaggtttc
      901 gattcccgac agaaaatcat cgaaggtcaa cagcacgcct tcggcaccag gcacgcttgc
      961 gacttcatct aacatgcgcg cgacactggc gtaagaaccg actaatgtcc ccatattgat
     1021 gtttaccgcc gaagtgggat cggccatctg acggacgttg gtgtcagtac cggagcgggt
     1081 atctttctga ctttgttcgg ttagccagct taacgcctct tcatccgcgc ccgctttgta
     1141 gtgttcccat ttggcgcgag cggcatcgtc ggtttcatcg gcaatcacca taaacaacac
     1201 ataagagcca acgtcgcgcc cggtttgctc tgcggcctgt ttcatccgca cagcggtcgg
     1261 ggcgaaagcc gtgggtgtat ttacgccttt gccgaaacag aagttgaaat cggcatactg
     1321 agcggagaac gccatgccag cgtcgctttg cccggcgcag atcactttca tggggacact
     1381 cggttgcgga ctgacgcgac aatcattcat ggtgaaaaaa tcgcctt
//
LOCUS       DAEEKQ010000373         1424 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.382, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000373 DAEEKQ010000000
VERSION     DAEEKQ010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.382"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(321..1403)
                     /locus_tag="KLK57_004470"
     CDS             complement(321..1403)
                     /locus_tag="KLK57_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005099906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HBE0894636.1"
                     /translation="MFRIPVNQWTLATAALGGVFLVSGLILLMNYNHPYTFTAQKAVI
                     AIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKTL
                     RAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDALV
                     KGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLIR
                     PGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGKL
                     TSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYSD
                     HFSHVSVMRKVLLRMTSWMHYLYLDH"
BASE COUNT          373 a          382 c          325 g          344 t
ORIGIN      
        1 tatagattaa atatttttta aatgtgactg tcatccttca gataataaag ccatcatcgc
       61 aatagatgga aaagcactcc tgctcttttt tgattagagc cgtcgcagga ggaacaactc
      121 atatcatcat tgcgatctcg acgatgtata gtctcatcct gcggcagaac aagacggata
      181 aaaaatccaa tgagagcaca ctcatccaga acttattaac ataatgaata ttaaagaaaa
      241 atcatcgcag ccaatgaagt aatatgcaaa aataattagc ccttgcaggg agcaaagttc
      301 cataatctcc cccttccccc tcaatgatcc aaatacaaat aatgcatcca actggtcatt
      361 cttagcagca ccttccgcat caccgaaaca tggctgaaat ggtcggagta aactgctacc
      421 tgggcgtaaa tgccgtcggg taaggcatcg atatcatcgt tagggtccag ttcaatggtt
      481 cccagcacgc cgtccgtgcc gggaacgacc gttaatgatt gcaatacccc ctgcgcctga
      541 taagaaccgc ctggcacgac aggtaaaata ctagtcaatt tgccgtggaa cacctgccca
      601 ggtagcgcgt taaacaccac ttccgcatca tcgccaggtt tcagacgtaa cagcgagttt
      661 tgccgaaatt gggcgacaat ttgccgtttt tgctcgggga tgaagaccat caccggacgc
      721 agcggcaagg cagctgcgta tgtacctggg cggatcagta cctgagtaac gtagccattg
      781 ctcggcgcgc gaatgacagt ctgctcaagg ttatattttg cttcagtaag ttgcgctctt
      841 aagctcacaa tctgcgattg ctcgccgtta accatactgt cgagctggct ctggatctgc
      901 gcctgctccg ccaccgagcc tttcaccagc gcatcctgcg cgaggaaatt ttgccgcgca
      961 tcgtcgatgt cacgttccga gaacggattc accgccgcct ggctgccttt caggtaacgt
     1021 tgataatttt taaacagacg gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct
     1081 tcagtgagct gcgcacgcag cgtctttata ttatgcgtcg ccgtcatcag gtcagcctga
     1141 agtctgtcaa ctcgcgcctg gtaacgaacc gggtcgagct taaaaagcac ctcgcccttt
     1201 tgaataagct gattattctt gtcagtgact tcagtaacaa ttcccgtcac ctgtggcgtg
     1261 atagggatcg ctatcactgc cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc
     1321 aacaaaatca aaccactcac cagaaacacg cctcccagcg ccgccgtcgc cagcgtccac
     1381 tgattcaccg gaatacgaaa gattttaaag accgcccacg ccag
//
LOCUS       DAEEKQ010000374         1421 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.383, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000374 DAEEKQ010000000
VERSION     DAEEKQ010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1421
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.383"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>1421
                     /gene="rhsC"
                     /locus_tag="KLK57_004471"
     CDS             <1..>1421
                     /gene="rhsC"
                     /locus_tag="KLK57_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsC"
                     /protein_id="HBE0894637.1"
                     /translation="LVLTTQAQRAEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIR
                     LSAVWLTHDPEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVA
                     HRHTGRPEIRYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLNRREVLHTQGEAG
                     LKRVVKKEHADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRAS
                     AFYYNHHSQLTSATGPDGLEMRRKYDESGRLIQETAPDGDIIRYRYDNPHSDLPCATE
                     DATGSRKTMTWSRYGQLLSFTDCSGYVTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQ
                     LIAVKDTQGHETRYEYNAAGDLTTVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYD
                     AAGRVIRLTSENGSHTTFRYDVLDLLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTH
                     WHYDEADRLTHRTVNGETAERWQ"
BASE COUNT          338 a          422 c          436 g          225 t
ORIGIN      
        1 ctggtactga ccacgcaggc gcagcgggca gaagaagccc ggcagcaggc catttccggc
       61 gggacggaac cgtccgcttt tcctgatacc ctgccgggtt acaccgaata tggccgggac
      121 aacggcatcc gtctgtctgc cgtgtggctg acgcacgacc cggaataccc ggagaattta
      181 cctgccgcgc cgctggtgcg ctatggctgg acgccgcgcg gcgaactggc ggcagtgtat
      241 gaccgcagca atacacaggt gcgcagcttt acttacgatg ataaataccg gggccggatg
      301 gtggcgcacc gtcacacggg ccggccggaa atccgttacc gttacgacag cgacgggcgg
      361 gtgacagaac agctaaaccc ggcaggctta agctacacgt atcagtatga gaaggaccgc
      421 atcaccatca ccgacagcct gaaccgccgt gaagtgctgc acacgcaggg tgaagccggg
      481 ctgaagcggg tggtgaaaaa ggaacacgcg gacggcagcg tcacgcagag tcagtttgat
      541 gcggtgggca ggctcagggc acagacggat gccgcaggcc ggacgactga gtacagcccg
      601 gatgtggtga cgggactcat cacgcgcatc accacgccgg atggcagggc atcggcgttt
      661 tactataacc accacagcca gttaacgtca gccaccgggc ctgacgggct ggaaatgcgc
      721 cgcaaatatg atgaatccgg ccgtctgatt caggaaactg cccctgacgg cgatatcatc
      781 cgctaccgtt atgataatcc acacagtgac ttaccctgcg caacggaaga tgccaccggc
      841 agccggaaaa ccatgacgtg gagccgttac ggtcagttgc tgagcttcac cgactgttcc
      901 ggttatgtaa cccgttatga ccatgaccgc ttcgggcaga tgacggcggt gcaccgcgag
      961 gaagggctga gtcagtaccg cgcatacgac agccgtggac agttaattgc cgtgaaagac
     1021 acgcagggcc atgaaacgcg gtatgaatac aacgccgccg gtgacctgac caccgtcatt
     1081 gccccggacg gcagcagaaa cgggacacag tacgatgcgt ggggaaaagc tatctgtacc
     1141 acgcagggcg gtctgacgcg cagtatggaa tacgatgctg ccggacgggt catccgcctg
     1201 accagtgaaa acggcagcca caccaccttc cgttacgatg tactcgacct gctgatacag
     1261 gaaaccggct ttgacggccg cacacagcgt tatcaccacg acctgaccgg caaacttatc
     1321 cgcagcgagg atgaggggct ggtcacccac tggcactatg acgaagcaga ccgcctcacg
     1381 caccgcaccg tgaatggcga aaccgcagag cggtggcagt a
//
LOCUS       DAEEKQ010000375         1417 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.384, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000375 DAEEKQ010000000
VERSION     DAEEKQ010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.384"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..649)
                     /locus_tag="KLK57_004472"
     CDS             complement(<1..649)
                     /locus_tag="KLK57_004472"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HBE0894638.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTT"
     gene            complement(813..908)
                     /locus_tag="KLK57_004473"
     CDS             complement(813..908)
                     /locus_tag="KLK57_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894639.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(1022..1189)
                     /locus_tag="KLK57_004474"
     CDS             complement(1022..1189)
                     /locus_tag="KLK57_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894640.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(1192..1356)
                     /locus_tag="KLK57_004475"
     CDS             complement(1192..1356)
                     /locus_tag="KLK57_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HBE0894641.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT          377 a          347 c          330 g          363 t
ORIGIN      
        1 tagtggtgtg cgagtcagta ccaacgagtg tatccggata agcaatccat tcaccgtcct
       61 gcaattcact ccacactgct ttgccgagat attcgaggtt aacctgatgg caaatgcctg
      121 tgcctggcgg cacgacgcta aaccgactga acgcttgctt tccccatttc aggaacacat
      181 aacgttcgtg gttgcgctcc atttccaggc gtacgttttc ttcaaatgcc tcatcatcac
      241 caaaacgatc gacggtcacc gagtggtcaa tgaccaggtc gaccggtgag agcgggttaa
      301 cctttgcagt atcgccgccg aggcgtttaa ccgcttcgcg cattgccgcc agatcaacaa
      361 cggcaggtac gccggtaaag tcctgcatca acacccttgc cgggcggtag gcaatttcac
      421 ggtcagcatg ggcatttttc agccatcctg ccagcgcgtg gatatcctct tcggtaaccg
      481 agttaccatc ctgccagcgc agcaggtttt cgagcaaaac tttgagtgac ttgggtagac
      541 gggtgatatc gcccagtgat ttagcagcaa gcggcaggct gtagtagtgg taagttttat
      601 ctttggcctg caacgtgtcc ttactggctt ctcgtagggt tgacgacata gctcctcctt
      661 aatgacaggg ttgcgaagcc ctgattctct tcagggtgaa tattaaagat aacacaaaca
      721 tcgcgtaacg atttgataac aacccaaatt gataaaagag gagaaacctt gtggcaaagc
      781 aaatgacaac cccgccgcag cggggtcaag gatcagtgag taatgatcca gaggagttcg
      841 gcccagaaga gcatcgacag agaaaacgcg cccatccagg accaatattt catatttgtg
      901 ttgatcatag gctacaccct taacggttat tattttgtgt tgagataact caagctgtta
      961 ttgctgcggt gtattaaaag ccctttaatt cattaaaaat gggtaataag aatgatgata
     1021 attattgttt cggctgatca tcttcagcaa acagttctat aaaggcttgc tgctgcgatg
     1081 ttagttgcca gtcgtctgga ataacgacgg aacattcccg tttcgtttct gtttcattgt
     1141 cactatgctg gcataatggt gacgactcca gttgctcctg accgaccata gttatcccca
     1201 cactttccaa atcagcagtg cgacaaccac ccagaaaagt gcagaaccaa gaaaaaccgc
     1261 cagccaggct ttacgtttta attcaggatc gcggcggggt tcctgatttc cagaaggcat
     1321 tgctaacctc atataatcgc catcacttat cagcaagacg ttgaacgatt ggtgtaatta
     1381 atcatacgat gagacaaatc ctaaagaaac tttagtc
//
LOCUS       DAEEKQ010000376         1394 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.385, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000376 DAEEKQ010000000
VERSION     DAEEKQ010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1394
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.385"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..863)
                     /locus_tag="KLK57_004476"
     CDS             complement(<1..863)
                     /locus_tag="KLK57_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE0894642.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTAQPDYHGPRIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIW
                     HAFEHEEHANT"
     gene            complement(1179..>1394)
                     /locus_tag="KLK57_004477"
     CDS             complement(1179..>1394)
                     /locus_tag="KLK57_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HBE0894643.1"
                     /translation="DGTLYGATILGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLT
                     HILSEHFENVKTKVQGTVVMFSASGKK"
BASE COUNT          389 a          247 c          309 g          449 t
ORIGIN      
        1 aaggtgttgg cgtgctcttc atgttcaaaa gcatgccata tctgtgatac atcagattgt
       61 ttgttttccg ggaaccatgc tgtcacggca tcagccaggg ggcgatggag tgtattctgt
      121 tgtccgtcac tcatggagaa gtaaatccgt gggccgtggt agtccggttg ggcggtttgt
      181 tgtaacaaag tttgcattat tctgggagat agttgatttt ctctaagatt gacaacgcta
      241 tcagaactga ggccaagtat gctttccgga attgccgtga tccggttctg attaagccag
      301 aatattctta taatatgatc atctctttct ggaaaatctg gtattacttc caaagcattt
      361 ctggctgcat caagtaattc caatgacatt ggtaacctcg gaagtacaga caggtgattg
      421 tcacttacat taatatattc tagtgacgca ggtaattcag gaagtgcgaa caaatggtta
      481 tcactcacat ttatatattc cagcgacact ggtaattcag gaagtgcaga taattgatta
      541 ctgcttgcat tcagctcttt caatgccctt ggtagctcgg ggagcattga tagttggtta
      601 ttgcttacat taatttcatc aagattgtca ggcaatcgtg gtagagatct gagacctaaa
      661 caagataagt ccaacgatgt ttcactgttc tccagacata attggagccg ggtaaaagca
      721 gtttccctgt tttctccgga aatgctgttt ttagtccatt caacccattc ggagagataa
      781 ttattgtgaa cattgtcgat tgatgtagtt ctgtaaaaag agacgtttcc agtggatagg
      841 gggggattat ttacaggaag cataataacc tcgcagaagg atattctgat aaaatgtgat
      901 actgggaaat aataagctga aataaaatgt ttcaggattg aaatttattg ttttcatttt
      961 aaggaagaga tgttgattta actgtaaaaa aacgatacta ttttcttaga tggtaaatgt
     1021 ctcgcctgtg ctatattttg tttttcggtt agttaaatat cgtacgttca tatttgaacg
     1081 ttctgccgga atgcattatc aatagaggta aagtcgcaac cccaaatcgt aaaggaaacc
     1141 gtagcacgtc ttaagcaaga acgtgctgcg gttggatgct attttttccc tgaagcggaa
     1201 aacattacta cagtaccttg aaccttggtt ttaacattct cgaaatgctc tgagagtata
     1261 tgtgttaagc cttcttcgga atcttttgtg tttgaaaaga tgcctttctg attgtaaatg
     1321 cgcatcagtt tttgaccgaa gctattgtgc acaactccat cgccaagaat tgtggctccg
     1381 tatagagttc catc
//
LOCUS       DAEEKQ010000377         1393 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.386, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000377 DAEEKQ010000000
VERSION     DAEEKQ010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1393)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1393)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1393
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.386"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..459
                     /locus_tag="KLK57_004478"
     CDS             <1..459
                     /locus_tag="KLK57_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HBE0894644.1"
                     /translation="LNAMASQRTKEARGGELQINELLPASLDAAKAHGLTERDVYEAT
                     DYYKTPRGQQPGGATKMLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRD
                     GLEVYGRAMVSQDRQLVSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            477..1115
                     /locus_tag="KLK57_004479"
     CDS             477..1115
                     /locus_tag="KLK57_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HBE0894645.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPVTDGDKPLFM"
BASE COUNT          444 a          282 c          307 g          360 t
ORIGIN      
        1 ttgaatgcaa tggcgtcgca acgaaccaaa gaggctcgtg gtggagaatt acagatcaac
       61 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      121 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      181 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      241 ttactgactc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      301 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttca
      361 ttacccgatt tctcacatta tgaactgtat gacaaaccgg aagcagttca ggaggcgctg
      421 gaaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      481 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      541 cagataaaca aaccattaaa caaactttat ttgcccgtca gccagcaaaa atccacaaac
      601 cctatccaac taatagcaag attattatgg gagatgtact gaatcatgca gcactgaagc
      661 aggccatgca gggacaggac gttgtgtatg ccaaccttac gggggaagat ttagatattc
      721 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      781 tttccctcgg tatttatgat gaagttccag gaaaatttgg cgaatggaat aatgcagtta
      841 ttggtgagcc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
      901 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
      961 cctcacgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1021 tcacagatat catcgataaa cctgagaaac acattggtga gaatataggt atcaatcaac
     1081 ccgtgactga tggtgacaag ccattattca tgtaaaccaa ttaaatagtt accatcgtaa
     1141 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tccgatgcga
     1201 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacttaca
     1261 tctaagtcag cataattcta atatctgcac atcaataaaa atgcaaacaa cttccggcaa
     1321 aaaaagtgat acaagatcga tgacatagcg ttgaagaaaa ggatttgata atccgcaaaa
     1381 tttgcacaac act
//
LOCUS       DAEEKQ010000378         1376 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.387, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000378 DAEEKQ010000000
VERSION     DAEEKQ010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1376
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.387"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            3..983
                     /locus_tag="KLK57_004480"
     CDS             3..983
                     /locus_tag="KLK57_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001346030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HBE0894646.1"
                     /translation="MVGVPVPDNIHPMIKDEAGVWSWRTPVLKGNLYEYFFNVDGVRS
                     IDIGTAMTKPQRQVNSSMVLVPGSYLDTRSVAHGDLIAITYHSNALQSERQMYVWTPP
                     GYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPDTETDA
                     KGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALAGLSQG
                     GYQALVSGMNHLESFGWLATFSGVTTTTVPDEGVAARLNDPAAINQQLRNFTVVVGDK
                     DVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            998..>1376
                     /locus_tag="KLK57_004481"
     CDS             998..>1376
                     /locus_tag="KLK57_004481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HBE0894647.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGL"
BASE COUNT          359 a          315 c          363 g          339 t
ORIGIN      
        1 tagtggtggg tgttccggtt ccggacaata ttcacccgat gatcaaagac gaagcaggag
       61 tctggtcgtg gcgcacgcct gtcctgaaag gcaacctgta tgagtatttt ttcaatgttg
      121 atggtgtacg cagcattgat ataggcaccg caatgactaa gcctcagcgc caggttaact
      181 ccagtatggt tctggtgcca ggcagttatc tggatacgcg ttctgttgcg catggtgatt
      241 tgatcgccat aacttaccac tccaacgcat tgcaatctga acgtcagatg tatgtctgga
      301 ccccgccagg atacaccggc atgggcgagc ctttgccagt gctctatttc tatcacggtt
      361 ttggtgatac cggacgttcc gctatcgatc aggggcgtat cccgcaaatc atggataacc
      421 tgctggctga agggaaaatt aaaccgatgc tggtggtgat cccggatacc gaaaccgatg
      481 cgaagggcat tattcccgaa gatttcgtgc ctcaggaaag acgtaaagtc ttttatccgc
      541 tgaatgctaa agcggcagat cgcgaactga tgaacgatat tatcccgctg attagcaagc
      601 gttttaatgt ccgtaaagat gccgatggtc gcgcgctggc agggctttca caaggcgggt
      661 accaggcgct ggtttccgga atgaatcatc tggaaagctt tggctggctg gccacattca
      721 gtggtgttac cacgacaacc gtaccggatg aaggtgtcgc ggcccggctg aacgatccgg
      781 cagctattaa ccagcaacta cgtaatttta ctgtggtcgt tggtgataaa gatgtcgtaa
      841 ccggcaagga tatcgccggg ctgaaaactg agcttgagca gaaaaaaatt aagtttgatt
      901 accaggaata tcccggcctg aaccatgaaa tggatgtctg gcggcctgcc tatgcagcct
      961 tcgtacagaa attatttaaa taaaataagg cattaagatg aaattaatca ttaccgaaga
     1021 ttaccaggaa atgagccgtg tcgcggcaca ccatctgctt ggttatatgt cgaagacgcg
     1081 tcgtgttaac ctggcaatta ccgccggtag cacgcccaaa ggcatgtatg aatacctcac
     1141 caccctggtt aaaggtaagc cctggtacga taactgctat ttctataatt tcgatgaaat
     1201 tccctttcgc ggcaaagagg gagaaggcgt aacgattacc aatctgcgta atctgttttt
     1261 cacccctgcg gggatcaaag aagagaatat ccagaagctc actattgata actaccgcga
     1321 gcatgatcag aaactggcgc gtgaaggcgg cctggatttg gtggtgctgg gattag
//
LOCUS       DAEEKQ010000379         1363 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.388, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000379 DAEEKQ010000000
VERSION     DAEEKQ010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.388"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(226..>1363)
                     /locus_tag="KLK57_004482"
     CDS             complement(226..>1363)
                     /locus_tag="KLK57_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBE0894648.1"
                     /translation="TWYGWDGDRLTTIQTGTTRIQTVYQPGSFTPLLRIETENGEQAK
                     ARHRSLAEVLQEDTGVTLPAELSVMLGRLERELRAGAVSAESEAWLAQCGLTAEQMAA
                     QLEAEYIPERKLHLYHCDHRGLPLALISPEGETAWQGEYDEWGNLLGETSAQHLQQSL
                     RLPGQQYDEESGLYYNRNRYYDPLQGRYITQDPIGLRGEWNLYKYPLNPVRFIDSLGL
                     KFHVNGDPSDFNQAVEYLKQDSQMKETIDFLSSSEETINIEYIEGTNVRFNSNNMTIY
                     WNSRASLFCSTELNSKSQSPALGLGHEFTHAQYCLLDKENFMALLSRTDKKYENKEEA
                     RVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
BASE COUNT          328 a          402 c          249 g          384 t
ORIGIN      
        1 cataaagatc tctaatttca ttcaccacca ctttatatct gtatatttca ttaagacctt
       61 ccttgctaag cggaactaac gtggtaacat acgatggaag attaattatc tctatcgttt
      121 tcacgctatc tattccacac aattcattca ctcgccccat ccccaatacc gaaccagaaa
      181 atagagacaa taaaaccatc aaatatttca tcataaaaaa cctcactatt ctggcgtacc
      241 cgttattttc atcgtctgca acggtccatc cacccgataa aaaggcagtc cactatgagc
      301 tcctctggta cactcaccta aagttttcgc cgcacgcgat tcaattattg taataaccct
      361 tgcctcttct ttattctcat atttcttatc tgttcggctt aaaagagcca tgaaattttc
      421 cttatcaaga aggcaatact gcgcatgagt aaactcatgt cccaaaccta atgcaggcga
      481 ttgtgatttc gagtttaatt cagttgaaca aaataaagat gctctactat tccaatatat
      541 cgtcatatta ttcgaattaa atctaacatt ggttccttca atatactcta tatttatagt
      601 ttcttcagag cttgataaaa aatctatagt ttctttcatt tgtgaatctt gttttaaata
      661 ctcaacagcc tgattaaaat cacttggatc accgttaaca tggaatttta aacccaaaga
      721 atctataaac cgaacagggt ttaatggata tttatacaga ttccattcac ctctcagccc
      781 tatcggatcc tgggtgatat accttccctg caacgggtca tagtaccggt tgcggttgta
      841 gtacagcccc gactcctcat catactgctg ccccggcaga cggagtgact gctggaggtg
      901 ctgcgcgctg gtttcgccca gcaggtttcc ccattcgtca tactccccct gccacgccgt
      961 ttccccttcc gggctgatga gcgccagcgg cagtccccgg tggtcgcagt ggtaaagatg
     1021 aagtttcctc tccgggatgt attccgcctc cagctgcgcg gccatctgct ccgccgtcag
     1081 cccgcactgc gcaagccatg cctcactctc cgcactcacc gctcccgcgc gcagttcccg
     1141 ctccagcctc cccagcatca ccgacagctc cgccggtagc gtcacacccg tgtcctcctg
     1201 caacacctcc gccaggctcc ggtgccgcgc cttcgcctgt tcgccattct ctgtttcgat
     1261 tctcaggagc ggcgtgaagc tccccggctg gtataccgtc tggatacgcg tggtgccggt
     1321 ctgtatcgtc gtcagcctgt caccgtccca cccgtaccat gtc
//
LOCUS       DAEEKQ010000380         1352 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.389, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000380 DAEEKQ010000000
VERSION     DAEEKQ010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1352)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1352)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1352
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.389"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..485)
                     /gene="umuC"
                     /locus_tag="KLK57_004483"
     CDS             complement(<1..485)
                     /gene="umuC"
                     /locus_tag="KLK57_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HBE0894649.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGMGIAQTKTLAKLANHAAKKWQRQT
                     GG"
     gene            complement(485..904)
                     /locus_tag="KLK57_004484"
     CDS             complement(485..904)
                     /locus_tag="KLK57_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HBE0894650.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            1277..>1352
                     /locus_tag="KLK57_004485"
     CDS             1277..>1352
                     /locus_tag="KLK57_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin activation protein"
                     /protein_id="HBE0894651.1"
                     /translation="MTEIVADKTVEVVKNAIETADGALD"
BASE COUNT          405 a          327 c          278 g          342 t
ORIGIN      
        1 ccccccgtct gccgctgcca tttttttgcc gcatgattgg caagcttagc cagcgttttg
       61 gtctgggcga tccccatacc aacagtaaga tgggtacgtt gtagcaccgt tgcgcgaatt
      121 tctctgccaa aatcagtcag atcgcgacaa ttacgcacac ctgtcagatc gcagaatgcc
      181 tcatcaatac tgtaaatctc gacgcggggc gatagttctt ccagcgtcga catcacccga
      241 ttgctcatgt ctgcgtaaag ctcataattg ctgctaaagc aaaccacacc acagcgacga
      301 aacagatctt tttgtttgaa ccagggatcg cccattttaa cacccagcgc ctttgcctca
      361 gcgtttcggg cgataacgca accgtcatta ttcgatagca caaccaccgg tttaccccat
      421 aaatcagggc gaaacaccgt ctcacagctg gcataaaacg cgtttacatc acagagagca
      481 aacatcagcg catcgcctta acgacgtgga tcaccacacc aaagacatcc agcgtatctt
      541 cgctactgat ggtaatgggc gagtacgcac tgttcatggg aataagctgt accgtcgggc
      601 gtagttgcaa ttttttcacc gtaaactcgc cgtcaacagc agcgatgaca atatcaccat
      661 ggctggcggt aatagcgcta tcgacaatca gtaaatcacc gtcactaatt ccaccatcaa
      721 tcatagaatc accacttgct ttgacgaagt aagtcgcgct gggatgctgg atcaacagtt
      781 gattcagatc gatgcgctgt tcgacgtaat ctgctgccgg tgaaggaaag ccacactgaa
      841 caagatcgct aaatagcgga aaagtcacaa tttcgcggag atccgcaggc ttgataaaca
      901 acataataat ctgcctgaag ttatactgtt tttatataca gtaatctgtt cttgccagca
      961 gatcaatact gattcaggct atcaatattt gtcgctgcat agactgctga tttttcgttc
     1021 tcttatcctg tgctcacgtg gccttctggc gacgacgctc atccagcaga aatgaaaaat
     1081 atcacccggc aaaaaaatag aatagaagca ttcgccataa tgacattaaa cattgtttga
     1141 tatttatcat attaatagaa ataaagacat tgacgcatcc cgcccggcta actatgaatt
     1201 agatgaagta aaatttatta atagttgtaa aacaggagtt tcattacaat ttatatattt
     1261 aaagaggcga atgattatga ctgaaatcgt tgcagataaa acggtagaag tagttaaaaa
     1321 cgcaatcgaa accgcagatg gagcattaga tc
//
LOCUS       DAEEKQ010000381         1346 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.390, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000381 DAEEKQ010000000
VERSION     DAEEKQ010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.390"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..184)
                     /locus_tag="KLK57_004486"
     CDS             complement(<1..184)
                     /locus_tag="KLK57_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease subunit"
                     /protein_id="HBE0894652.1"
                     /translation="MIFNRLKLAVDRVIAAFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLG"
     gene            complement(229..1215)
                     /locus_tag="KLK57_004487"
                     /pseudo
     CDS             complement(229..1215)
                     /locus_tag="KLK57_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
BASE COUNT          372 a          297 c          272 g          405 t
ORIGIN      
        1 cacctaataa ccctacccag atcatcaaaa atcgtgccag ctcatcggtt aatgtacttg
       61 gctggttaag tacgtagcgg ctaaatactt gccagactac gcatacaacc agagcaagca
      121 tgacggcgac agaaaacgcg gcaatgacgc ggtctaccgc aagtttcagg cggttaaata
      181 tcattgtaag catcttggaa aatcagggcc agagactggc ccggaatatt attgagcagc
      241 gttgtcgaac ttctctaaca aagcagcttg ttttggatct tttttgaagt cgtcaaacag
      301 aggctgaact gcggcacgga atggtgcttt atccactttt acaatttcac cccccatagc
      361 tttcgcctga gcacgtgtgt cagcgtcaat tttttcccac aatttttgtt gataagcttc
      421 ggatttcttc gctgctgttt cgaggatttg tttctgttct ggggttaatt tattccatgt
      481 tttggtggag ataaccaaaa agtcaggaat tgaggcgtgt tcatcttcag aaaaaacttt
      541 ggcaatttca atatgtcgag tctgcaccca ggaaggcacg ttattttctg cgccatctac
      601 tacaccttgc tgcatcgcag tataaacttc gccaaatgaa attggagtgg gtgaaccacc
      661 catcagttca atcattttga tggtggtcgg acttggctga acacgaattt tcagcccttt
      721 aagatcttct ggcttagtaa tcggtttttt cgcatagaaa ctgcgtgtac ccgctacata
      781 agctgataaa gcaaaaaagc ctttgtcttt tgttgagtcc ataatttctt taccaacttc
      841 accaaaaaca actttgttga agtgagcctg atctttaaat aagaacggca aattatagat
      901 agcataaata ttatcaaaag actccagatc actggcggaa cctttggtca tatccagtgc
      961 gccattttgc aacaattcca tagtttatcg cgcattaccc atctggctgc tgggataaat
     1021 acggatcacc atattacctt ctgacagctg ttttacctct ttcgccagct cttcaaaact
     1081 ctgatgtaca acatgacttc tttccaggtt gtgtgctaac tttaacgtca ctttttctgc
     1141 ggcgtttacg ccagtgctaa ccagagccag caaggatgcg cagactaatg agcgggtaaa
     1201 tgacctcatt ttcataaaaa aactccctgt tgaaggatgg aaattggttg atatctaatt
     1261 gtgcttatca gtgctttgtg cgacaaattg gttaacctat ctgacaactt gtctatgttt
     1321 atcacaactt gaaataaagt cagaaa
//
LOCUS       DAEEKQ010000382         1341 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.391, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000382 DAEEKQ010000000
VERSION     DAEEKQ010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1341
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.391"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..184
                     /locus_tag="KLK57_004488"
     CDS             <1..184
                     /locus_tag="KLK57_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894653.1"
                     /translation="TISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQ
                     GKRRCQQQFTSQEVQN"
     gene            194..>1341
                     /gene="yicI"
                     /locus_tag="KLK57_004489"
     CDS             194..>1341
                     /gene="yicI"
                     /locus_tag="KLK57_004489"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HBE0894654.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPG"
BASE COUNT          335 a          318 c          351 g          337 t
ORIGIN      
        1 cacgattagc atcattattg cgctattcac gattgttccg gcgatctgtt atttgctgag
       61 cgcgattatc gctaaacgct actactcact cacgacgcac aatctgaaaa ccgttatgga
      121 acagctggct cagggtaaac gccgttgcca gcaacaattc acctctcaag aagtgcagaa
      181 ctaaggaacg gcaatgaaaa ttagcgatgg aaactggttg attcaacctg gcctcaattt
      241 gattcacccg cttcaggtgt tcgaggttga acagcaggat aatgaaatgg tggtctatgc
      301 tgccccccgt gatgtgcgtg aacgtacctg gcagcttgat acgcctttat ttacgctgcg
      361 ctttttctcc ccacaggaag gtattgtcgg tgtgcggatt gagcattttc agggggcgct
      421 gaataacggt cctcattatc cgctcaatat tttgcaggac gtgaaggtca caatcgaaaa
      481 cacagaacgt tatgctgagt ttaaaagtgg caacttaagc gcgcgtgtca gcaaaggtga
      541 gttctggtca ctggattttc tgcgcaacgg cgaacgtatt accggtagtc aggtgaaaaa
      601 taatggctac gtgcaggaca cgaataatca acgcaattat atgtttgagc ggcttgatct
      661 tggcgttggc gaaacagttt acggtctggg agagcgcttt actgccctgg tgcgcaatgg
      721 ccagacggta gagacctgga accgggacgg cggcacaagt actgaacagg cgtataaaaa
      781 tatcccgttc tacatgacta accgtggtta tggggtactg gtcaatcatc cccagtgtgt
      841 ctcttttgaa gtgggatcgg agaaagtctc caaagtgcag ttcagcgttg agagtgaata
      901 tctcgaatac tttgttatcg acggcccgac gccgaaagcg gtacttgatc gttatacccg
      961 ctttactggt cgtccggcgc tgccgcccgc gtggtccttc ggcctgtggc taaccacttc
     1021 atttaccacc aactacgacg aagcgacggt aaacagcttt atcgatggta tggcggaacg
     1081 caatctgccg ctgcatgttt tccactttga ctgtttctgg atgaaagcct tccagtggtg
     1141 cgattttgag tgggacccgc tgactttccc tgacccggaa gggatgatcc gccgcctgaa
     1201 agcgaaaggg ctgaaaatct gcgtctggat taacccctat atcggtcaaa aatcccccgt
     1261 ctttaaagag ttacaagaga aaggctattt actcaaacgc ccggacggtt cgctatggca
     1321 gtgggataaa tggcagccag g
//
LOCUS       DAEEKQ010000383         1334 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.392, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000383 DAEEKQ010000000
VERSION     DAEEKQ010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1334)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1334)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1334
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.392"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            112..1212
                     /locus_tag="KLK57_004490"
     CDS             112..1212
                     /locus_tag="KLK57_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HBE0894655.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKSFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPGLADRKPLWQYAEKLDDTYCAGCHAPIAADHYTVNAWPSIA
                     KGMGARTSMSENELDILTRYFQYNAKDITEKQ"
     gene            1237..>1334
                     /locus_tag="KLK57_004491"
     CDS             1237..>1334
                     /locus_tag="KLK57_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HBE0894656.1"
                     /translation="MTLTRREFIKHSGIAAGAQVVTSAAPLPAWAE"
BASE COUNT          379 a          285 c          341 g          329 t
ORIGIN      
        1 tctttatcga ttatttcagg cagtgttgtg ccgagttatg caagcggtta attcgttgtg
       61 tatttaatta tacaatgatt ccggtgttct ggaatttatt tagaggaatc tatgcgaggg
      121 aaaaaacgca ttgggttatt gtttttgctg atagcggttg tggttggtgg cggcgggtta
      181 ttgctggcgc aaaaagcctt acataaaacg tcggatacag cattttgcct ttcctgccac
      241 tcgatgagta aatcttttga ggaatatcag ggaactgtcc acttttcgaa ccagaaaggg
      301 atacgtgcgg aatgtgcaga ttgccatatt ccaaagtcag ggatggatta tttatttgct
      361 aaattaaaag catctaaaga tatttatcat gaatttgtta gcggcaaaat agacagtgac
      421 gataagttcg aaactcatcg ccaggaaatg gccgaaacag tatggaaaga attaaaagca
      481 actgactctg caacgtgccg tagttgccat tcttttgatg ccatggatat tgcctcgcaa
      541 agtgaatctg cgcagaaaat gcataacaaa gcacaaaagg gcggcgaaac ctgtatcgat
      601 tgtcataaag gcattgccca ttttccgcca gaaataaaaa tggatgacaa cgcggcgcat
      661 gagctggaaa gtcaggccgc tacttcagtg actaatggcg cacatattta tcctttcaaa
      721 acttctcgca taggcgagct ggctaccgtg aatcctggta ccgatctcac cgtcgttgat
      781 gccagtggca aacagccgat cgttctgttg cagggttatc aaatgcaggg cagtgaaaac
      841 acgctctacc tggcggcagg tcaacggctg gcgctagcca cattaagtga agaaggtatc
      901 aaggcgctca cggtaaacgg ggaatggcag gctgacgaat acggcaatca atggcgtcag
      961 gcgtctttac agggtgcgct taccgatccc ggattagcgg accgtaaacc gctatggcaa
     1021 tacgctgaaa aacttgacga tacctattgc gctggttgtc atgcccctat tgccgccgac
     1081 cattacaccg tcaatgcgtg gccgtccatt gccaaaggaa tgggggcacg aaccagcatg
     1141 agcgaaaacg aactggacat tttaacgcgg tatttccagt acaacgccaa agatattacc
     1201 gagaaacagt gatcctcgtt gatcagggag gaagttatga cattaacaag acgtgaattt
     1261 attaaacaca gcggtattgc cgctggagcg caggtggtga catcggcagc accgctcccc
     1321 gcatgggcag aaga
//
LOCUS       DAEEKQ010000384         1294 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.393, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000384 DAEEKQ010000000
VERSION     DAEEKQ010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.393"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..1112
                     /locus_tag="KLK57_004492"
                     /pseudo
     CDS             1..1112
                     /locus_tag="KLK57_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001763881.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          355 a          262 c          399 g          278 t
ORIGIN      
        1 gacctggtac gggtgggacg gtgacaggct gaccactgta cagacccaac agacaagaat
       61 ccagacggta taccagccgg gaagcttcac gccgctcctg agaatcgaaa cagagaatgg
      121 cgaacaggcg aaggcgcggc accggagcct ggcggaggtg ttgcaggagg acacgggggt
      181 gacgcttccg gcggagctgg cggtgatgct ggggaggctg gagcgggaac tgcgtgcggg
      241 tgcggtgagt gcggagagtg aggcatggct tgcgcagtgc gggctgacgg tggagcagat
      301 ggaaagccag atggaggcgg aatacatccc ggagcggagg ctgcatcttt accactgtga
      361 ccaccgggga ctgccgcagg cgctcatcag cccggaaggg gaaacggcgt ggcgcgggga
      421 gtatgacgag tgggggaacc agcttaatga ggagaacccg catcacctgt accagccgta
      481 ccgtttgccg gggcagcagt atgatgagga gtcgggactg tattacaacc gtcaccggta
      541 ctacgatccg ttgcaggggc ggtatatcac ccaggatccg atagggttga aggggggaat
      601 aaatctatac acgtatccac tggcaccaat acgttacacc actggggtta gaacgagtta
      661 taagtgtata tgggcctccc gctccagacc gggcaggtgc tgaaactcct cttgttctta
      721 ctgatatgac tggaggagtg actatttatt atgatcctga aacaggtgat tcaatgactt
      781 ttgatagtag caatagaatt gatcgtcgtt ctcaacgtgg tgctggtgat ccatatacag
      841 gtgaagtcgt tggttgtgag acaaatggat caggaatatc ggcagcatat ggcacaacga
      901 agatctatac tacagatact agagcaagat ggctacatgg tgggggatca tcgttgcgcg
      961 atccttatgc tcctcgacaa ggctggaaac caactatggg atgtacccgt gcacagaatg
     1021 aagatgtaga tgagttgtgt aagaaagtga caagttggat gtattcgcat cctggagaga
     1081 gaattagata tgaacgtttt aagacacgtt aatgcgatta acatgggagt gtgcattcta
     1141 attttggtta ttggatgtgc aaatgctaaa gttcttaaac gatgtatatt acccgctcct
     1201 gatggtggcg tagaaaaact atcaaaaccc aacttacatg gagtaattaa aaaagttgat
     1261 ttgaacacaa atgaagcgga aattaaaata aaag
//
LOCUS       DAEEKQ010000385         1289 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.394, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000385 DAEEKQ010000000
VERSION     DAEEKQ010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.394"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..277)
                     /locus_tag="KLK57_004493"
     CDS             complement(<1..277)
                     /locus_tag="KLK57_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HBE0894657.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHIYGKSTFREYC"
     gene            complement(498..1229)
                     /gene="bluR"
                     /locus_tag="KLK57_004494"
     CDS             complement(498..1229)
                     /gene="bluR"
                     /locus_tag="KLK57_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HBE0894658.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWNRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAAASLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPHPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          332 a          293 c          286 g          378 t
ORIGIN      
        1 aacagtattc ccgaaatgta gattttccgt aaatatgaag caatgcggcc tcttttattt
       61 tagcagggtt cttggtcgta ctaactttta acaggttcct ggttcctctt aataacaaaa
      121 ccgtgtcatc gtgagtaata attctgatgt tatccgtagc cagataataa atgtaatgtg
      181 caatacggtg atgttttaat tctgaataaa accaggagaa gttttgctct tttctcactt
      241 gctcaaacat cttttgaaaa acaacgacct gatccatcag gataataacc tcttgttagt
      301 tgtgagactg cgtagtgtgc acgatcggtt ttaccacttc aatctggtct gtcctttggc
      361 tgtgatatgt acagagtgtg atagagggat tacctgaatt cttccggtga gcattttgca
      421 acggaccagc tccggtacaa atgctgttgt gggttcagat tataacattc tgtctaaggg
      481 gcgggataaa ggtgaaatta gggggcatga aagatgactt tataaccttg ctcaccccaa
      541 tgttgtaaaa gttcgttttg ccttctcgtt ggtgccatgc ctgtccagac aatcaatgtt
      601 tgcgtcggga acagttccgg gtgcggcgat tcaatgggat cagcaagaac agaaatgtgc
      661 catcctgaca atgataaacg ccatgcttcc agccacaatc gtgctctctc ttcaacattc
      721 cacgccatca gaatggcttc tttaccgggc ttacggcgca tttcgaaaag tgaggctgct
      781 gcatattcaa ttaatgcgcc gtcaaacata ctgctcataa tgcgggaggt gttgtgatca
      841 agcacgagac gctggcgaac aggaaggtaa acatgattaa tcaattgatc tactgggtac
      901 tctcgaccca gtgaaataat tctcgcgcgt agtttggcag gattagccat acgaagaatt
      961 gacatcatct cttcttgcag gcggttccag tcatcttccg tatcctggct ggtggtttcc
     1021 agtaatgctt taactttgcc tacagggacg ccattactta tccaacgctt gatctcttcg
     1081 atgcgttgta tgtcttcttc atcaaagagt cggtgtccgc cttcactgcg ctgtggtttt
     1141 aacaaaccgt agcggcgttg ccaggcccgg agagtgacag gattaatccc gcaacgttca
     1201 gcaacatcac caatgctgta ataagccaca attcctcctt gcggtcataa atctccgccg
     1261 cctgtacacg acccaataat actttgtac
//
LOCUS       DAEEKQ010000386         1282 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.395, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000386 DAEEKQ010000000
VERSION     DAEEKQ010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.395"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            132..1151
                     /locus_tag="KLK57_004495"
     CDS             132..1151
                     /locus_tag="KLK57_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISEc11 family transposase"
                     /protein_id="HBE0894659.1"
                     /translation="MKYTPVGVDIAKHVIQIHFINEHTGEVVDKQLRRQDFLTFFGNR
                     EPCLIGMEACGGSQHWARELTKLGHKVRLLQARFVKAFVMGNKNDVMDARAIWMAVQQ
                     PGKEIAVKTEEQQSVLVLHRTRMQLVKFRTAQINALHGTLLEFGETIHKGRAAMEREF
                     PEALERMKERLPPYLIMVLENQYNRLNELDSLIEDIEKQLTSVARQNETCKRLLDIPG
                     VGPLIATAAVATMGEASAFKSGREFAAYVGLVPKQTGSGGKVRLLGISKRGDTYLRTL
                     FIHGARAVALVAKEPGPWITELKKRRPASVAIVAMANKLARTVWAITAHDRKYDRNHV
                     SIRPY"
BASE COUNT          368 a          285 c          357 g          272 t
ORIGIN      
        1 cacacaatta aacacggagc agtatgatgg ttaaaagggt gaaactggca ggtgtggagc
       61 ctcgcagatt tccggcatca tagtgcccaa tacgggatgc ataaagctcc ctcagactgg
      121 agactccaca aatgaaatat acaccggttg gcgttgatat cgcaaaacat gtcattcaga
      181 ttcacttcat caatgagcac acaggtgaag tggttgataa acagttgcgt agacaggatt
      241 ttctgacgtt cttcggcaac cgtgagccat gcctgattgg tatggaggcc tgtggaggtt
      301 ctcagcactg ggcacgggaa ctgacaaaac ttggtcataa agtccggttg ttgcaggccc
      361 gcttcgttaa ggcattcgtc atgggcaata agaatgatgt gatggatgcc cgggctatct
      421 ggatggcggt tcagcagccg ggtaaagaaa tcgccgtaaa aacagaagaa cagcagtcgg
      481 tactggttct gcaccgtacc cgcatgcaac tggtgaagtt ccggaccgca caaattaatg
      541 ccctgcacgg gacgttactg gagtttggtg aaaccatcca caaaggccgg gcagcgatgg
      601 agcgggagtt ccccgaagca ctggaacgga tgaaagagag actgccaccg tatctcatta
      661 tggttctgga aaaccagtac aaccgactga atgagctgga ctcactgata gaggatattg
      721 aaaaacagct taccagcgtg gcgaggcaga atgaaacctg taagcggttg ctggatattc
      781 ctggcgttgg accacttatt gcgacggcag cggtggccac catgggggaa gcatcagcgt
      841 ttaaatcggg gcgagagttc gccgcatatg ttggtctggt tccaaaacaa actggctccg
      901 gagggaaagt acgtctgctg gggataagca aacgtggtga cacttatctc aggacattat
      961 ttatccacgg tgcaagagcg gtggcattag tagctaaaga gcctggcccg tggataaccg
     1021 aactgaaaaa acgtcgtcca gccagtgtgg caatcgtcgc catggcaaac aagctggcac
     1081 gaacagtatg ggcgataacc gcccatgacc gtaagtatga caggaaccac gtcagtatca
     1141 gaccatatta atcgctgata ccattaaaca atgaactctt aacaaaaggg tgaatgctga
     1201 aaggttgcta tggcggccag agtgatgaca aagacaggta agaccgtgac tcactaaacc
     1261 tgaacagtat tttgggcttg aa
//
LOCUS       DAEEKQ010000387         1281 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.396, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000387 DAEEKQ010000000
VERSION     DAEEKQ010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1281
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.396"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>1281
                     /locus_tag="KLK57_004496"
     rRNA            <1..>1281
                     /locus_tag="KLK57_004496"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          317 a          283 c          404 g          277 t
ORIGIN      
        1 cgatgggaaa caggttaata ttcctgtact tggtgttact gcgaaggggg gacggagaag
       61 gctatgttgg ccgggcgacg gttgtcccgg tttaagcgtg taggctggtt ttccaggcaa
      121 atccggaaaa tcaaggctga ggcgtgatga cgaggcacta cggtgctgaa gcaacaaatg
      181 ccctgcttcc aggaaaagcc tctaagcatc aggtaacatc aaatcgtacc ccaaaccgac
      241 acaggtggtc aggtagagaa taccaaggcg cttgagagaa ctcgggtgaa ggaactaggc
      301 aaaatggtgc cgtaacttcg ggagaaggca cgctgatatg taggtgaagt cccttgcgga
      361 tggagctgaa atcagtcgaa gataccagct ggctgcaact gtttattaaa aacacagcac
      421 tgtgcaaaca cgaaagtgga cgtatacggt gtgacgcctg cccggtgccg gaaggttaat
      481 tgatggggtc agcgcaagcg aagctcttga tcgaagcccc ggtaaacggc ggccgtaact
      541 ataacggtcc taaggtagcg aaattccttg tcgggtaagt tccgacctgc acgaatggcg
      601 taatgatggc caggctgtct ccacccgaga ctcagtgaaa ttgaactcgc tgtgaagatg
      661 cagtgtaccc gcggcaagac ggaaagaccc cgtgaacctt tactatagct tgacactgaa
      721 cattgagcct tgatgtgtag gataggtggg aggctttgaa gtgtggacgc cagtctgcat
      781 ggagccggcc ttgaaatacc accctttaat gtttgatgtt ctaacgtgga cccgtgatcc
      841 gggttgcgga cagtgtctgg tgggtagttt gactggggcg gtctcctcct aaagagtaac
      901 ggaggagcac gaaggttggc taatcctggt cggacatcag gaggttagtg caatggcata
      961 agccagcttg actgcgagcg tgacggcgcg agcaggtgcg aaagcaggtc atagtgatcc
     1021 ggtggttctg aatggaaggg ccatcgctca acggataaaa ggtactccgg ggataacagg
     1081 ctgataccgc ccaagagttc atatcgacgg cggtgtttgg cacctcgatg tcggctcatc
     1141 acatcctggg gctgaagtag gtcccaaggg tatggctgtt cgccatttaa agtggtacgc
     1201 gagctgggtt tagaacgtcg tgagacagtt cggtccctat ctgccgtggg cgctggagaa
     1261 ctgagggggg ctgctcctag t
//
LOCUS       DAEEKQ010000388         1279 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.397, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000388 DAEEKQ010000000
VERSION     DAEEKQ010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1279)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1279)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1279
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.397"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            6..1279
                     /locus_tag="KLK57_004497"
                     /pseudo
     CDS             6..1279
                     /locus_tag="KLK57_004497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          308 a          376 c          402 g          193 t
ORIGIN      
        1 cgtagggtga agccgggctg aagcgggtgg tgaaaaagga acacgcggac ggcagcgtca
       61 cgcagagtca gtttgacgcc gtgggcaggc tcaggacaca gacggatgcc gcaggccgga
      121 caacagaata cagcccggat gtggtgacgg gcctcatcac gcgcatcacc acgccggatg
      181 gcagggcatc ggcgttttac tataaccacc acagccagtt aacgtcagcc accgggcctg
      241 acgggctgga aatgcgccgc aaatatgatg aatccggccg tctgattcag gaaaccgccc
      301 ctgacggcga tatcacccgc taccgttatg ataatccaca cagtgactta ccctgcgcaa
      361 cggaagatgc caccggcagc cggaaaacca tgacatggag ccgttacggt cagttgctga
      421 ccgtcactga ctgttccggt tatgtaaccc gttatgacca tgaccgtttt ggtcaggtga
      481 cggcggtgca ccgcgaggaa gggctgagcc agtaccgcgc atacgacagc cgtggacagt
      541 taattgcggt gaaagacacg cagggccatg aaatgcggta tgaatacaac gccgccggtg
      601 acctgaccgc cgtcattgcc ccggacggca gcagaaacgg gacacagtac gatgcgtggg
      661 gaaaagccat ctgtaccacg cagggcgggc tgacgcgcag tatggaatac gatgctgccg
      721 gacgggtcat ccgtctgacc agtgaaaacg gcagccacac caccttccgt tacgatgtac
      781 tcgaccggct gatacaggaa accggctttg acggccgcac acagcgttat caccacgacc
      841 tgaccggcaa acttatccgc agcgaggatg aggggctggt cacccactgg cactatgacg
      901 aagcagaccg cctcacgcac cgcaccgtga agggtgaaac cgcagagcgg tggcggtacg
      961 acgaacgcgg ctggctgaca gacatcagcc atctcagcga agggcaccgg gtgacggtgc
     1021 attacgggta tgatgagaaa ggccggctga ccggtgagcg tcagacggtg catcacccgc
     1081 agacggaagc actgctctgg cagcatgaga ccagacatgc gtacaacgcg caggggctgg
     1141 cgaaccgctg tataccggac agcctgtccg ccgtggaatg gctgacctac ggcagcggct
     1201 ggctgtcagg catgaaactc ggcgacacac cgctggtgga gtacacccgc gaccgcctgc
     1261 accgggaaac gctgcgcag
//
LOCUS       DAEEKQ010000389         1268 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.398, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000389 DAEEKQ010000000
VERSION     DAEEKQ010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1268)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1268)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1268
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.398"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..381)
                     /locus_tag="KLK57_004498"
     CDS             complement(<1..381)
                     /locus_tag="KLK57_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894660.1"
                     /translation="MADKAILWALISASTKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTCYLLSKGKIIRPYLKNLNPLQLAADCIETVNK
                     IKDKNKKIIDINSVNICSDDKNIKL"
     gene            complement(470..>1268)
                     /locus_tag="KLK57_004499"
     CDS             complement(470..>1268)
                     /locus_tag="KLK57_004499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBE0894661.1"
                     /translation="QMQNQMDPVYTPARKIHLYHCDHRGLPLVLISTEGATEWCAEYD
                     EWGNLLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGW
                     NFYQYPLNPITDIDPLGLATCLYSITLSMLSCVSDTQNDDNSYDVLTIPVASGNNGNN
                     MQCKNNPGCTHLQNRGPIPQGVWSWNVNGPGATNRKPNGIRLVPSANTETYNRDGFLT
                     QSCLNAFGPSLGPRFCSEGCITGSSNDMQKLNELIFSEPDSTLTVTD"
BASE COUNT          340 a          274 c          241 g          413 t
ORIGIN      
        1 caattttata tttttatcat cactacaaat attaactgaa tttatatcaa tgattttttt
       61 attcttatct tttattttat ttactgtttc aatgcaatca gcggcaagtt ggagtggatt
      121 tagatttttc agatatggtc ttatgatctt tcccttactt aataaatagc atgtatagga
      181 ttcggaaagt ccagcatcta ttttgtacct cattatatta gataaggatg tgagaaattc
      241 cttgttatca ttcgcagcca tatatgccag tccgagttca gcctctgcgg ccttacaggc
      301 aaaatagctt agtgaacacg cttttcgtcc ctctttagta ctggctgata ttagtgccca
      361 taatattgct ttgtctgcca aatctaactc tctctgaaat tgtcttgcag taactgcact
      421 cgcagaaata gagaaaaaaa tacaattgtt aataactttg ccatattttt tagtccgtaa
      481 ctgttaatgt gctatctggc tctgaaaata ttaactcatt taatttttgc atgtcattag
      541 atgaaccagt tatacaaccc tcggaacaga atcgaggtcc taaagacgga ccgaaagcgt
      601 ttaaacaact ttgtgttaaa aatccatcac gattatatgt ttctgtattt gctgacggta
      661 ctaaacgtat accattaggt tttctattgg ttgctccagg accattaaca ttccagctcc
      721 ataccccttg aggtattgga cctctatttt gtaagtgtgt acaaccagga ttatttttac
      781 attgcatatt attaccatta ttaccagagg cgacaggaat agtaagcaca tcataagagt
      841 tatcatcatt ttgcgtatct gaaacgcatg aaagcatact taaagttatg ctgtataaac
      901 aggtcgctaa tcccaacggg tcaatatcag ttatcggatt caacggatac tgataaaaat
      961 tccatccccc cttcagtcca atcggatcct gagtgatata tcgcccctgc agcgggtcat
     1021 aatagcggtg gcggttgtaa tacaggccgg actcctcatc atactgctgc cccggcaggc
     1081 ggataagctg ctgcagctga tgcgggttct cttcattcag caggttgccc cactcatcgt
     1141 attctgcgca ccattctgtt gccccttccg tgctgataag caccagcggc aggccgcgat
     1201 ggtcgcagtg gtacaggtgg atttttcgcg ccggcgtgta caccgggtcc atctggtttt
     1261 gcatctgc
//
LOCUS       DAEEKQ010000390         1264 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.399, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000390 DAEEKQ010000000
VERSION     DAEEKQ010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.399"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(23..>1264)
                     /locus_tag="KLK57_004500"
     CDS             complement(23..>1264)
                     /locus_tag="KLK57_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016237897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0894662.1"
                     /translation="IKHIIIAERAHQESLVKQKRIAQVWNHKTQLARELKKPMGKQAP
                     GWLELSEDGSYYIVDEDKASLVNIIYDKRLSGMSMFAICKWLNEQGYLTINQRKVRIS
                     KTKKPDGNWSALSVKHILTSRSVLGYLPAKISTEDRKTVLREEIEGFYPQIVTDSKFY
                     AVQQLLEETGKGKTSSGEHWLYVNILKGLIRCKCGLVMTPTGIRKPVYQGTYRCNGNK
                     ESRCSYGTVSRKLLDTQLCSRLFSKLSQLHDEATDTAKLDELQRRLNTVDGELEKLTE
                     TLIQLPNITQIQEALRVKQEEKDELIVQLSREKARVKSVSSLDLSGLDMESVEGRTEA
                     QIIIKRLVKEIVVSGNEKLVDIYLHNGNMIRGFPLDGKDDHTLTLEEATDEMQSLDDM
                     LIFGEPVTRIYPAGDMEEVDA"
BASE COUNT          334 a          298 c          227 g          405 t
ORIGIN      
        1 cagtgaaccc taacaaaaaa tactaagcgt ccacctcttc catatcacca gcaggataaa
       61 tccttgttac tggttcacca aaaataagca tatcatctaa tgactgcatc tcatcggttg
      121 cttcttcaag cgttaaggtg tggtcatctt taccatcaag aggaaaacca cgaatcatat
      181 ttccattgtg aagatatata tccaccagct tttcattacc agaaacaaca atctctttca
      241 ccagacgctt aataataatc tgggcttcag ttctcccctc tacagactcc atatcaagac
      301 cgcttaaatc caagcttgat acagatttta ccctcgcctt ttctcgtgat aactgaacta
      361 tcagttcatc tttttcttcc tgtttaaccc gtagtgcttc ttgaatctga gtgatgttag
      421 gaagctgtat cagcgtctca gtgagttttt ccagttcacc atcaacggtg tttaatcttc
      481 tttgtagttc atctaattta gctgtatcgg tagcttcatc gtgtagctgt gacagtttgc
      541 taaacagacg actacaaagc tgtgtatcaa gtaactttct ggacactgta ccgtagctac
      601 aacgggactc cttattacca ttgcaacggt aagtaccttg ataaacgggt ttacgtatcc
      661 ctgtaggagt cattacaagc ccacacttac aacggataag ccccttcagt atgttcacat
      721 atagccagtg ttcaccgctg gatgttttcc ccttccctgt ttcctctaac agttgctgta
      781 cagcgtaaaa cttagagtct gtgacaatct gaggatagaa cccttcaatt tcttcacgta
      841 acactgtttt acggtcttct gtagaaatct tagcgggaag gtaacctaat acacttcgtg
      901 aagtcaggat gtgtttaacg cttaatgctg accagttgcc atccggtttc ttagtcttgg
      961 atattctgac cttacgctga ttaatggtca ggtaaccttg ttcgtttagc catttacaga
     1021 tagcaaacat cgacatacca gaaagacgct tgtcatagat gatattcacc agtgacgctt
     1081 tatcttcatc aacgatgtag tatgaaccat cttctgatag ctctaaccat ccgggggctt
     1141 gcttacccat aggcttctta agttctcttg caagctgtgt tttatggttc cagacctgag
     1201 ctatacgctt ttgcttaaca agagattctt ggtgtgctct ctcagctatg atgatgtgct
     1261 taat
//
LOCUS       DAEEKQ010000391         1260 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.400, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000391 DAEEKQ010000000
VERSION     DAEEKQ010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1260
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.400"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..164)
                     /locus_tag="KLK57_004501"
     CDS             complement(<1..164)
                     /locus_tag="KLK57_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="involved in the transport of nitrate and nitrite;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter"
                     /protein_id="HBE0894663.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSA"
     gene            complement(487..1260)
                     /locus_tag="KLK57_004502"
                     /pseudo
     CDS             complement(487..1260)
                     /locus_tag="KLK57_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310100.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
BASE COUNT          408 a          189 c          250 g          413 t
ORIGIN      
        1 gcgctaaata gcatccagac acagaaggca agaagtagac aactgactga tatccagagg
       61 tttcttcgag caatatgttt tcctttattt tcccagaagg ccggattttc tggtttccag
      121 tcgcgtaaaa gataacgact atttttctca ttttgcagtg ccatattgtt cctcacatgc
      181 acacattggt aatgaaaaaa gacaaaacag gaggtaaggc gcaatagcca gttattagaa
      241 ttaaggatga attgggtgaa gtgctgattg aaagaataga taagaaagcg taaccgcggg
      301 ggcagaatgg ggattaagta gccagatatg tgttactaaa tgtaactaac aagctaacct
      361 tcaaacgggg ttaatctttg aacgtatgtc atgtttcaac ccttcagatc gtgaatctaa
      421 agggttacat attaactata ttatcaacaa gaccagacac ttatagaatt agtgatgatt
      481 tattcactaa aaaggccatg aactggagga aaacgatctg tccagaaggt ctatcggttc
      541 ctctcttatt gcctgattgt aataatcgtt attacagtaa gtattgttta tttcatctaa
      601 ggtcatgttt ttaactttta tattcctctg gtttacaact tcctctgtct tgtaagtgtg
      661 tatattatat ggggtatatg ctatttttaa taggatagtg aaacaattcg ttatccacat
      721 ctattccgtc agagtgaact tcaaatgcac caggcgaaag aattgccagg ccagtattaa
      781 gcttaagtgt tggggaaaac aaattaaata acatgagctg catagtaggg caattgttct
      841 tcccagaaac atttaaaaaa tttaagtttt tgctctgttt tattatggga aataaagttc
      901 caatatcgga tgtattatta gtatcaatgt gggttatttt attgttgtcc agtcttaaac
      961 ttttaataga tggaagacaa gtcaggaaat caagatttat atttttcaac tggttgttag
     1021 cagcgtttaa tgtctgaaca ttgcagaagt tgctatagaa tattttaatt tcagaaagca
     1081 ggtttttgtt aataagtaac gtattcaagt tgggaaataa gacattgtgt aaacttgtta
     1141 gtttattatg ggatagatcc agcagtaaga gttaattttt attacctgca acaatatcag
     1201 ataattgatt atggctgaga ttcaggtatt gcagacattc gcaagattct aattgcacaa
//
LOCUS       DAEEKQ010000392         1243 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.401, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000392 DAEEKQ010000000
VERSION     DAEEKQ010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1243
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.401"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..784
                     /locus_tag="KLK57_004503"
     CDS             <1..784
                     /locus_tag="KLK57_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBE0894664.1"
                     /translation="AKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLNYGIYVN
                     QQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGINITNQLS
                     DTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSESETELQG
                     NRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIGVVGQGS
                     RLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            797..1243
                     /locus_tag="KLK57_004504"
                     /pseudo
     CDS             797..1243
                     /locus_tag="KLK57_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          400 a          249 c          236 g          358 t
ORIGIN      
        1 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
       61 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      121 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      181 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      241 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      301 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      361 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      421 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
      481 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
      541 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
      601 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
      661 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
      721 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
      781 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
      841 tgtgcaaagt gctccctgtg cattgaccaa tgtcggggaa caacgtggaa catatatact
      901 caagccactg agtatgaaag gtaatctcac cgccgaaaaa attgaaaaca ccggaaatat
      961 cattgagtgt gcagacttac aagagacgac caacgccatt aaagttcatt ttttaacaca
     1021 gaacgcttta aggtttaaag tcggaagtcg tcattatatt attaatttcc cctttgaatc
     1081 tgacggtaaa aaaaaataga gcttccagga aggtcatctt ataatttaga agaaatgcta
     1141 tctgcgataa atttcaccat tccatactct atcgcatctg tatcagatag ttccaacacc
     1201 gcagatattt ttccaggaac tccttttcct ttgttgttcg aaa
//
LOCUS       DAEEKQ010000393         1233 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.402, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000393 DAEEKQ010000000
VERSION     DAEEKQ010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1233
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.402"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(1..761)
                     /locus_tag="KLK57_004505"
                     /pseudo
     CDS             complement(1..761)
                     /locus_tag="KLK57_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          308 a          299 c          308 g          318 t
ORIGIN      
        1 aaagtctcca tggtacagac ccgtgcgcca tcaggaacgg tttcggtgaa tctgatgttg
       61 ccgcgttcgt cgaagtgaac aaccagggtg cggccatcgc ctggaattat tttgtcagtg
      121 ggtggggtgt tattctggcg cagttcggcc tccatgcgat cgaactcagc aatgtaggct
      181 tctttaaatg cagcggcttt tttgccagtg aatcccatca ccaggaaaac gaagccgttt
      241 ttggtgattt ggtacatcgg gcgtttttcg cctttggcgt cagtgtaggt gacgtcctca
      301 aaattgaggg cgttaaattc aggtgagcat tcagtatttg cgatggcacg taacacattg
      361 tcgtgacgct tgccaaagaa ctctgcgatc gcaatagacg tagtgacagc gcgaccattt
      421 tcgatggtta cgtcagggtg agaaagggca gggatagtag ccatgatggc atcctcgagt
      481 gataagttaa ttaactcacc accgaggttt tccacgacca taagggtggt gagacgtaca
      541 ggggtggaaa taccggtcac tcgagaaccc ggccagtctt gcgactgccc tgcacatccc
      601 accataattt gaatggagct gtgcattacg cataaaaaaa ccgcttcagc gcggctatgc
      661 gctcaagtaa cgttcgggtt tccacgcccg gcacccgttt tataaggtgc aggtgcacta
      721 taattccacc cgttctggtt ttcaatagct acattcaaca ttttctcttt cctttcatca
      781 ccgaagtgaa ctttgttgat gcggtgcctg gtgcctccag gtgacgttaa ccagttaaca
      841 attaacgccg gatacaggga aacccataac cacccgatac gggggaacta atcgcttttt
      901 aactgtgccg cgtgcgctga gccgcattca ccgcatcaca aaattcactt taaaaagggc
      961 ggacatcagc cagcaatgaa actgatgccg ccaaaggtac caatcaacat ggagtgttgt
     1021 ggcgtggttg tcacttaagc gtatggtcaa cctgacaacc cggtgtcctc aacggggaag
     1081 gaataactcc gccatactta ccgccgcgtc atttcgcgtt gtgtgcctgc ttttaaccac
     1141 gtcaggcgag gtggtatcct tcttatcccg aataaccaag aaggaaatct atatgactaa
     1201 agaagaattt gtctcttata tttttgataa aac
//
LOCUS       DAEEKQ010000394         1219 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.403, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000394 DAEEKQ010000000
VERSION     DAEEKQ010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.403"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            205..552
                     /gene="tnpB"
                     /locus_tag="KLK57_004506"
     CDS             205..552
                     /gene="tnpB"
                     /locus_tag="KLK57_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HBE0894665.1"
                     /translation="MIPLPSGIKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTSDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            556..1060
                     /locus_tag="KLK57_004507"
                     /pseudo
     CDS             556..1060
                     /locus_tag="KLK57_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004201219.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1120..>1219
                     /locus_tag="KLK57_004508"
     CDS             1120..>1219
                     /locus_tag="KLK57_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HBE0894666.1"
                     /translation="MHTVADVMTGNDGEYCFHARTGKYGVYLKPGWH"
BASE COUNT          289 a          328 c          343 g          259 t
ORIGIN      
        1 gggaaagctg ctattacctt cttcagagag cccccagcta cttcctgtga ctctcgatgc
       61 agctgccgaa cagccagaat cgctcgcaga ggacccgaaa accctcagta tcagctgtga
      121 ggtaacgttc cggcacggga cgctccgctt caatggcaat gtcagcgaaa agctcctgac
      181 tctgctgata caggaactga agcgatgatc ccgttacctt ccgggatcaa aatttggctg
      241 gttgccggta tcaccgatat gagaaatggc ttcaacggcc tggctgcgaa agtacagacg
      301 gcgctgaaag acgatcccat gtccggccat gttttcattt tccggggccg cagcggcagt
      361 caggttaaac tgctgtggtc caccagtgac ggactgtgcc tcctgaccaa acggctggag
      421 cgtgggcgct tcgcctggcc gtcagcccgt gatggcaaag tgttccttac acaggcgcag
      481 ctggcgatgc tgctggaagg tatcgactgg cgacagccta agcggctgct gacctccctg
      541 accatgctgt aaatctcttt atcctggttg tcacagaata agcccggtaa aatacgggct
      601 tatgaacgac atctcttctg acgacatctt cctgctgaaa cagcgccttg ccgaacgtca
      661 gttgaaaacg aaaccgctgc tgaaatccct ggaaagctgg ctgcgtgaaa agatgaaaac
      721 cctgtcgcga cactcagaac tggcgaaagc gttcgcatat gccctgaacc agtggccggc
      781 gctgacgtac tatgcagatg atggctgggc tgaggcggac aataacatcg ctgaaaatgc
      841 gttgcggatg gtcagtctgg gccgcaaaaa ctacctgttc ttcggttcgg atcatggagg
      901 agagcgggga gcgctgctgt acagcctgat cgggacgtgc aaactgaacg gagtggagcc
      961 agaaagctac ctccgctatg tccttgacgt catagccgac tggccgataa accgggtcgg
     1021 cgaactgctc ccctggcgcg tagcactgcc gactgaataa cacattcccg tcaatacggt
     1081 tcttgctgca cgcttacggt aaacaccccg gaagtggtga tgcacactgt tgctgatgtg
     1141 atgacaggaa acgatggtga atactgtttc catgcgcgga ctggaaaata tggtgtgtat
     1201 ctgaaaccgg gctggcaca
//
LOCUS       DAEEKQ010000395         1181 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.404, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000395 DAEEKQ010000000
VERSION     DAEEKQ010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.404"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..1000
                     /gene="htrE"
                     /locus_tag="KLK57_004509"
     CDS             <1..1000
                     /gene="htrE"
                     /locus_tag="KLK57_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HBE0894667.1"
                     /translation="WASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDDSVYLS
                     FTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARVSYSVNTGYTM
                     NKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLTFSNDS
                     FSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDLENDVE
                     LKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNVIGNVG
                     QGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRCQIQ"
     gene            1017..1121
                     /locus_tag="KLK57_004510"
                     /pseudo
     CDS             1017..1121
                     /locus_tag="KLK57_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
BASE COUNT          358 a          236 c          276 g          311 t
ORIGIN      
        1 ctgggcttcc ggacaaaatc gtagcaatta ctctattggc tacagtaaca gtgcatcctg
       61 gggcagctac agtgtcagtg cccagcgttc atggaatgaa gacggcgaca ctgacgatag
      121 cgtttatctc agtttcacca ttcctattga aaaattactt ggcactgaac aacggacttc
      181 aggtttccag agtattgata ctcaaatgag cagtgacttt aagggtaata accaactcaa
      241 cgtcagtagc agtggctata gtgataacgc tcgcgtcagt tatagcgtaa atactggcta
      301 tacgatgaat aaagccagca aagatttgag ttatgttggg ggttatgcca gctatgaatc
      361 accgtgggga accctggcag gttcagtttc tgcaaatagc gataacagtc gtcaagtgtc
      421 tctcagcacc gacggtggtt ttgtattgca tagcggcggc ctgaccttca gtaatgacag
      481 ttttagcgac tccgatacac tggcggtagt tcaggctcca ggtgctcaag gagcgcgaat
      541 aaattatggc aacagtacta tcgatcgatg gggttatggt gtcaccagcg ctctttctcc
      601 ttatcatgaa aaccgtatcg cgctggatat caacgatctt gagaacgatg ttgaattaaa
      661 aagtaccagt gcagtagctg taccgcgtca gggttcagtc gtctttgctg attttgaaac
      721 cgtgcaaggg caatcagcca ttatgaacat cacacgaagt gatggtaaaa atattccatt
      781 tgctgcagat atttatgatg agcaaggcaa tgtcattggt aatgttggac agggtggaca
      841 agcatttgtt cgtggcattg agcagcaggg aaatatcagc attaaatggc tcgaagaaag
      901 taaacccgta agttgtcttg cgcattatca acaaagttca gaagcagaaa aaatagcgca
      961 atctattatt ctgaatggaa ttcggtgtca gattcagtaa ctacaaggaa cgtcaaatga
     1021 taaaaataac gccacataaa atagcgatac tgatgggaat attattatca ccatcagtat
     1081 ttgcaacgga tattaatgta gagtttacag ccactgtcaa agtagactgg ccccctgaat
     1141 ctccagacaa ccagtatcac ttaaataagt gatagtctta a
//
LOCUS       DAEEKQ010000396         1158 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.405, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000396 DAEEKQ010000000
VERSION     DAEEKQ010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1158)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1158)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1158
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.405"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            2..598
                     /locus_tag="KLK57_004511"
     CDS             2..598
                     /locus_tag="KLK57_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BLUF domain-containing protein"
                     /protein_id="HBE0894668.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPLSRRIIAFEAIVQKNEAHQP"
     gene            595..>1158
                     /gene="bluF"
                     /locus_tag="KLK57_004512"
     CDS             595..>1158
                     /gene="bluF"
                     /locus_tag="KLK57_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005117879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HBE0894669.1"
                     /translation="MAVGQRKDGEIYTADLKSKALAFAMAHALELGDKMISINLLPMT
                     LVNEPDAVSFLLDEIKANALVPEQIIVEFTESEVISRFDEFAEAIKSLKAVGISVAID
                     HFGAGFAGLLLLSRFQPDRIKISQELITNVHKSGPRQAIIQAIIKCCTSLEIQVSAMG
                     VATPEEWMWLESAGIEMFQGDLFAKAKL"
BASE COUNT          327 a          247 c          259 g          325 t
ORIGIN      
        1 aatgcttacc acccttattt atcgtagcca tatacgtgac gacgaacctg tcaaaaaaat
       61 cgaagaaatg gtttcgatag caaatcgcag gaacatgcag tctgacgtaa cagggatctt
      121 actgtttaat ggttctcatt ttttccagct tctggaaggt ccggaagaac aggttaaaat
      181 gatatatcgg gctatatgcc aggatccacg gcactataat attgttgagc tgctgtgcga
      241 ttacgcgcct gctcgccgtt ttggcaaagc gggaatggaa ttatttgatt tgcgcctgca
      301 cgagcgagat gacgttttac aggccgtatt cgacaaaggc acatcaaaat ttcagctaac
      361 ttatgatgac agagcgctac aattttttcg tacttttgtc cttgcaaccg aacaatcaac
      421 ctatttcgag atccctgccg aagactcctg gctttttatc gctgacggat ctgataaaga
      481 acttgattcc tgtacccttt caccaactat aaacgaccac tttgcctttc atcccttatc
      541 gcggcggata atcgcttttg aagccattgt gcaaaaaaat gaagcccatc agccatagcg
      601 gttgggcagc gtaaagacgg ggaaatctac acagcggatc tcaaaagtaa ggcgcttgca
      661 ttcgcgatgg cacacgcact tgagctcggt gataaaatga tttcaatcaa tctattacct
      721 atgaccctgg ttaacgaacc tgacgcagtc tcttttttac ttgatgaaat aaaggccaat
      781 gctctggtgc ctgaacaaat catcgttgaa tttactgaaa gtgaagttat atctcggttt
      841 gatgagtttg cagaagcgat taaatcgctt aaggctgtcg gtatcagtgt agcaattgat
      901 cattttggcg caggttttgc cggtttgtta ctcctgtcac gcttccagcc tgacagaatt
      961 aaaatcagtc aggaattgat taccaatgtt cataaaagcg ggccacggca ggcaattatt
     1021 caagcgatca taaaatgctg tacatcactt gaaattcaag tcagtgctat gggcgtggca
     1081 acaccagaag agtggatgtg gcttgaatcc gcaggaattg agatgtttca gggagatctg
     1141 tttgctaaag ctaaattg
//
LOCUS       DAEEKQ010000397         1157 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.406, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000397 DAEEKQ010000000
VERSION     DAEEKQ010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1157
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.406"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            6..407
                     /locus_tag="KLK57_004513"
                     /pseudo
     CDS             6..407
                     /locus_tag="KLK57_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            572..>1157
                     /locus_tag="KLK57_004514"
     CDS             572..>1157
                     /locus_tag="KLK57_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HBE0894670.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISLAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRV"
BASE COUNT          321 a          260 c          276 g          300 t
ORIGIN      
        1 ggtgacgagt ctcatcgtct atggaaacga cattcgtgct ggatgcactg gagcaggcgt
       61 tgtgggcccg tcgcccgtct ggcaccatcc atcacagcga taaaggctct cagtatgtgt
      121 cactggccta taaggcgcga ctaaaagaag caaaactgct ggcatcaaca gggagtactg
      181 gtgactcgta tgacaacgcg atggcagaga tcatcaaggg cctttacaaa gcggaggtaa
      241 tacaccgtaa gagctggaaa aaccggacag aagtggagct ggccacactc acgtgggtgg
      301 actggtataa caatcgacga ttgctggaaa ggctgggcca tatcccaccg gcagaagcag
      361 aaaaagctta ttatgcttcc atcggaaatg atgatctggc agcctgagtt cacagataaa
      421 acactctcca ggaaacccgg ggcggttcag ttcacaaaaa cacattaatg cagtaactat
      481 attttccttt ctggtgataa cgtcgggtta tcattagctt cttcagctat tttactttta
      541 tatcccttgt aaacatcatg gagaatccat agtgaataaa gtttattctc ttaaatattg
      601 ccccgtcacc ggggggctta ttgctgtctc tgaacttgcc cgcagggtaa taaaaaagac
      661 atgccgaaga ttaacgcata ttcttctggc tggcattcca gcaatctgtc tgtgttactc
      721 tcagatatcc ctggcgggta ttgtccgttc cgatattgcc tatcagattt atcgtgattt
      781 cgccgaaaac aaagggcttt ttgtacctgg tgccaatgat attccggtat atgataagga
      841 cggaaaactt gtgggaagac tgggtaaagc cccaatggcc gatttcagca gtgtgagctc
      901 aaatggcgtt gctacgcttg tatcgcctca gtatatcgtc agcgtaaagc ataacggagg
      961 atatcggagt gtgagctttg gtaatgggaa aaatacatat tcccttgttg accgtaataa
     1021 ccacccttct attgacttcc atgctccacg tctgaataaa ctggttacag aagttattcc
     1081 ctcagcggta acatcagaag gaaccaaagc caatgcttat aaatacactg aacgttacac
     1141 cgctttttat cgggtgg
//
LOCUS       DAEEKQ010000398         1148 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.407, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000398 DAEEKQ010000000
VERSION     DAEEKQ010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.407"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            247..492
                     /locus_tag="KLK57_004515"
     CDS             247..492
                     /locus_tag="KLK57_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894671.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPIVGIRQIRLIT"
     gene            complement(509..1129)
                     /locus_tag="KLK57_004516"
     CDS             complement(509..1129)
                     /locus_tag="KLK57_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HBE0894672.1"
                     /translation="MDPLHAVYLTVGLFVITFFNPGANLFVVVQTSLASGRRAGVLTG
                     LGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAWCSMRRQSTPQMST
                     LQQPISASWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTWARLMAWAGIVLAS
                     VIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGVFALRLIYEGVTQR"
BASE COUNT          346 a          274 c          273 g          255 t
ORIGIN      
        1 tttcgcagcc atgcggtgta taaaaaatga tctcatgcag atgttttgtg aatgtgtggg
       61 ttgacattca tatgaaaaaa atcataattc catcatgttt gtgtggggtc ttttctgtac
      121 cttacgcatc gcactcaagc ctgacagaaa atatgctgta aggctcatat caaaaaccgc
      181 cattagctca tcaggaagag cagacgacaa ccaggattgt tgtatggtac ggggttcgag
      241 gcctcgatgg cggtccaact tttcaaaact cttctgaatc agatcccctt attatcatcc
      301 ctgcaaagtg gaacattgcc attgtttggc tattcaggac gggtcagacc aatgaaaaaa
      361 gcccacacag gggagagtgg gctgaaatgg gaagctaaag actcaagtaa acttatcgga
      421 aataaggacc acgcattacg ggggctatca tcgccgatag tcgggattcg gcaaatcagg
      481 ttgattacgt agagagtaaa ttatctgctc accgctgcgt caccccttcg taaatcaggc
      541 gtagcgcgaa tacaccaata acccgactgg caacgcgttg catacgccca taagcacgac
      601 gcacagcggg caaagaaaac gcctgactaa gaaagactcg ccagataact gatgcgagca
      661 caatccccgc ccaggccatt aaacgtgccc atgttggtgt ttcggcattt aatgttactg
      721 agaaaatact gataaaaaat aaaacggttt gcgggttaga gagatcggta attaatccgc
      781 ggcgaaaaaa gacataccag gaggcgctaa tcggttgttg tagtgtgctc atttgcggtg
      841 ttgactggcg gcgcatgctg caccacgcaa accataagag ataagcgccg ccgacgattc
      901 tgataagcga aaaaatctcc tcacactgcg ttattagcgt tgcaagacca aacaaaccca
      961 acccggaata aaatgcatcg cccagcgcca cgcccagtcc ggtcagcacc cctgcgcgtc
     1021 gaccggaagc caggctggtt tgtactacca caaagagatt ggctcccgga ttaaaaaaag
     1081 taatcacgaa cagtcctacg gtcaggtaaa cggcgtgcaa aggatccata gtgatttcat
     1141 ccataaat
//
LOCUS       DAEEKQ010000399         1147 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.408, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000399 DAEEKQ010000000
VERSION     DAEEKQ010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1147
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.408"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..189
                     /locus_tag="KLK57_004517"
     CDS             <1..189
                     /locus_tag="KLK57_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HBE0894673.1"
                     /translation="VREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTN
                     NYRIALRKYQHYQRQTCT"
     gene            322..1122
                     /gene="tcyJ"
                     /locus_tag="KLK57_004518"
     CDS             322..1122
                     /gene="tcyJ"
                     /locus_tag="KLK57_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HBE0894674.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTADDLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
BASE COUNT          318 a          248 c          307 g          274 t
ORIGIN      
        1 gtgcgtgaat atgtcgttcg tctgcgccgt ttgggtaatc atctacacga gcaaaatatt
       61 tccctcgatc agctgcagga cggtttcctt gatgaaatcc tcgccccgtg gctgcccacc
      121 accagcacca acaattaccg cattgcgtta cggaagtatc aacactatca gcgtcaaacg
      181 tgtacctgac ttgtgcagaa atcctccagt ctgcctgctt ctgatatata ttaaataaga
      241 ataagatgta gcggagttgt ttttgtgttt acaaacaatg gctctacact gcaaacagac
      301 ataacaacat tcggggtgaa tatgaaatta gcacatctgg gacgtcaggc attgatgggt
      361 gtgatggccg tggcgctggt tgcgggtatg agcgttaaaa gttttgcaga tgaaggtctg
      421 cttaataaag tcaaagagcg cggcacgctg ctggtagggc tggaaggaac ttatccgccg
      481 ttcagttttc agggggatga cggcaaatta accggttttg aagtggaatt tgcccaacag
      541 ctggcaaaac atcttggcgt tgaggcgtca ctaaaaccga ccaaatggga cggtatgctg
      601 gcgtcgctgg actctaaacg cattgatgtg gtgattaacc aggtcaccat ttctgacgag
      661 cgcaagaaaa aatacgattt ttctacccct tacaccattt ccggtattca ggcgctggtg
      721 aaaaaaggta acgaaggcac cattaaaaca gccgatgatc tgaaaggcaa aaaagtgggt
      781 gtcggtctgg gcaccaacta tgaagagtgg ctgcggcaga atgttcaggg tgtcgatgtg
      841 cgtacctatg atgatgaccc gaccaaatat caggatctgc gcgtaggccg tatcgatgcg
      901 atcctcgttg accgtctggc ggcgctggat ctggtgaaga aaaccaacga tacgctggca
      961 gtaaccggtg aagcattttc ccgtcaggag tctggcgtgg cgctgcgtaa aggaaatgaa
     1021 gatctgctga aagcagtgaa tgatgcaatt gcggaaatgc aaaaagatgg cactctgcaa
     1081 gccctttccg aaaaatggtt tggtgctgat gtgaccaaat aatcagcata atgacaaaaa
     1141 agggcgc
//
LOCUS       DAEEKQ010000400         1117 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.409, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000400 DAEEKQ010000000
VERSION     DAEEKQ010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.409"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            84..1115
                     /locus_tag="KLK57_004519"
     CDS             84..1115
                     /locus_tag="KLK57_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS630-like element IS630 family transposase"
                     /protein_id="HBE0894675.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLAIKINEITGCQLNAGTVRRWLPSAGIVWRRAAPTLRIRDPHKDEK
                     MAAIHKALDECSAEHPVFYEDEVDIHLNPKIGADWQLRGQQKRVVTPGQNEKYYLAGA
                     LHSGTGKVSCVGGNSKSSALFISLLKRLKATYRRAKTITLIVDNYIIHKSRETQSWLK
                     ENPKFRVIYQPVYSPWVNHVERLWQALHDTITRNHQCSSMWQLLKKVRHFMETVSPFP
                     GGKHGLAKV"
BASE COUNT          303 a          285 c          301 g          228 t
ORIGIN      
        1 tagatcactt tgagggaact tagcccggat tgtgcgatct gatcaatcgc caaatcaaaa
       61 caaatcacca accggactga gcaatgccga tcatagcacc aatttcccgt gacgaacgac
      121 gcctgatgca gaaagccatc cataaaacac acgataaaaa ttatgcccgc agactgactg
      181 ccatgctgat gctgcaccgg ggcgaccgtg tcagcgacgt tgccagaacg ctctgctgcg
      241 cccgttcctc tgttggacgc tggattaact ggttcacgca gtcgggtgtt gagggactga
      301 aatcattacc tgccgggcgt gcccgtcgct ggccgtttga gcatatctgc acactgttac
      361 gtgagctggt aaaacattct cccggcgact ttggctacca gcgttcacgc tggagtacag
      421 aactgctggc aataaaaatc aatgagataa ccggttgcca gttaaatgcc ggaaccgttc
      481 gccgctggtt gccgtctgcg gggattgtgt ggcgaagggc tgcgccaact ctgcgtatcc
      541 gtgacccgca taaagatgaa aagatggcag caatccataa agcactggac gaatgcagcg
      601 cagagcatcc ggtcttttat gaagatgaag tggatatcca tcttaatccc aaaatcggtg
      661 cggactggca actgcgcgga cagcaaaaac gggtggtcac gccgggacag aatgaaaaat
      721 attatctggc cggagcgctg cacagcggga caggtaaagt cagctgtgtg ggcggcaaca
      781 gcaaaagttc ggcgctgttc atcagcctgc tgaagcggct taaagcgaca taccgtcggg
      841 cgaaaaccat cacgctgatc gtggacaact acattatcca caaaagccgg gaaacacaga
      901 gctggctgaa ggagaacccg aagttcaggg tcatttatca gccggtttac tcgccatggg
      961 tgaatcatgt tgaacggcta tggcaggcac ttcacgacac aataacgcgt aatcatcagt
     1021 gcagctcaat gtggcaactg ttgaaaaaag ttcgccattt tatggaaacc gtcagcccat
     1081 tccccggagg caaacatggg ctggcaaaag tgtagcg
//
LOCUS       DAEEKQ010000401         1104 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.410, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000401 DAEEKQ010000000
VERSION     DAEEKQ010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1104
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.410"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(68..1051)
                     /locus_tag="KLK57_004520"
     CDS             complement(68..1051)
                     /locus_tag="KLK57_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HBE0894676.1"
                     /translation="MSNDYPLVSIIIPTYNSSDYITETLTKLEKQTYPNFEIVIVNDG
                     SKDNTSNVLREYGLTHSRLIIINKENGGVSSARNTGIRKAQGQFICFMDDDDEIDPNY
                     LLKMYTRQHETGGDAIYCGLYGHYIKDGVTYSPINTEFNEGSLLFDFFYKKVRFHIGC
                     LFIRKQLLEENNLFFDEDLRLGEDLDFIYRLLITCDMYAVPYYMYKHNYRENSLMNSC
                     RTITHYRHESFAHERIYSSVMQLYKGNRKEEIHTLLSQNRAYHKTRYLWNVLLNGDFE
                     LLNQLVESNEKELNDCNLSGKRDKRRAKILASKNYILWRMVRLVNRKKNKR"
BASE COUNT          349 a          206 c          151 g          398 t
ORIGIN      
        1 tttcattcca acaaaatgac atatagtttc aacctatgct acgaggacgg attatttaat
       61 gcaagatcta acgtttattc tttttcctat ttaccagtct taccatcctc cagagaatat
      121 aattttttga tgctaatatt tttgctcgtc ttttatctct cttgccagaa agattacaat
      181 cattcaattc tttttcattg ctttcaacta attgatttaa tagttcaaaa tcaccattaa
      241 gtagaacatt ccataaataa cgagttttat gataagctct attttgactc aataatgtat
      301 gaatttcttc tttcctgtta cctttgtata actgcatcac agaagagtag attctttcgt
      361 gcgcaaatga ctcatgtcga taatgagtga tggttctaca tgagttcatt aaggaatttt
      421 ctctatagtt atgcttatac atataatatg gaaccgcata catatcgcat gtaattagca
      481 gtcgatagat aaaatccaga tcttctccta gtcgtaaatc ttcatcaaaa aaaagattat
      541 tctcttccag aagttgtttt cttataaaca agcaccctat atggaatcta acctttttat
      601 aaaaaaagtc gaaaagtaaa gatccttcat taaactctgt atttataggt gagtaagtaa
      661 caccatcttt tatataatgg ccataaagcc cacaataaat cgcatcccct cccgtctcat
      721 gttgtctggt atacatcttc agcagatagt taggatctat ctcatcatca tcatccataa
      781 aacatataaa ctgtccttgc gccttgcgga tacctgtatt cctggcagac gaaacaccgc
      841 cattttcttt attgataata attaatcgag agtgggttaa cccatactct ctcaaaacgt
      901 ttgatgtgtt atctttagaa ccatcattaa caataacaat ttcaaaattt gggtaagttt
      961 gtttttctaa tttcgttaga gtttcagtaa tgtaatcaga tgaattatac gtcggtatta
     1021 ttatggatac taaagggtaa tcattgctca tcgtgttctc ttgcctgaac taataaaata
     1081 tgatagtact catccttaat tatt
//
LOCUS       DAEEKQ010000402         1100 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.411, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000402 DAEEKQ010000000
VERSION     DAEEKQ010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.411"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..294)
                     /locus_tag="KLK57_004521"
     CDS             complement(<1..294)
                     /locus_tag="KLK57_004521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBE0894677.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEP"
     gene            complement(342..914)
                     /locus_tag="KLK57_004522"
     CDS             complement(342..914)
                     /locus_tag="KLK57_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HBE0894678.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
BASE COUNT          341 a          237 c          205 g          317 t
ORIGIN      
        1 aggttctaat cgtgatactg gcggaataac ggtaaacggc gctttgcttt tatcctcttt
       61 attaaatgga tctacccaag cctggatgag ataaggtatt ttttcttctt tgctaaatac
      121 agaaatagat ttatcagcat tattacttaa gtaaaccacc cgcgtaccac caatgacaac
      181 gcctgcataa gttaatgctg gttggcctaa aaaaacgctg agtataatcg cggtaaataa
      241 cttacatgaa actgttgttt taaagagcct gattaaacaa ctttttctgg acataacgac
      301 tccctggcca aagccaactg aataaaaagg cccttgataa attatttctt atattcgaca
      361 gtaaattgtg aatcagcgtt cgccgtacca gccgttacgg tcgcattggt tgccacatag
      421 cgtgcaataa acttcatttc agccttgcct tcagcatcga tagcggtata agcagagtcg
      481 ttataaagtt taacctgcga attatcggac tggttaaaaa tacgaatacc aacgccggtt
      541 gccgcaatcg cttgtcctgt accggtaaaa tcaccagggt tgcctgctgt aagcggcgta
      601 cccactttca ggtcgccgtc gccgccaggc gcttctgtac catcaaaacg aacggctgct
      661 tgagtgtacg ttgccgggca attttcaagt ttaatactga acttctgtgg cgacgccaga
      721 ctacctacac ctgaaaatgc atttttactg actttaccca gtgggacaat ttgatcggct
      781 gtatcggagg taacttcaca agtagtgtcg acaatttcac cgacgaagtg aacaacacca
      841 tcttctgcca gtgcatgccc ggccagcatt agagatgcca gagtgaatgc accgcctata
      901 atattacgct tcataaaaac tccattgata taatatatcc tttaccatga atcatcactt
      961 atattaataa gtgtattttt gattcaaagg aatagtacac caaagagacg gctttatcta
     1021 aaaataatat taattacgca aaaataaaat ataaattcat tgtttaaata ttatttaaac
     1081 agtaaaaaat caagtttgtt
//
LOCUS       DAEEKQ010000403         1088 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.412, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000403 DAEEKQ010000000
VERSION     DAEEKQ010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.412"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(270..1043)
                     /locus_tag="KLK57_004523"
     CDS             complement(270..1043)
                     /locus_tag="KLK57_004523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HBE0894679.1"
                     /translation="MHDQPLLRMSLPSIPSFVLSGLLLLCLPFSSFACATTSHISFSY
                     AARQRMQNRARLLKQYQTHLKKQASYIVEGNAQSRRALRQHNREQIKQHPEWFPAPLK
                     ASDRRWQALAENNHFLSSDHLHNITEVAIHRLEQQLGKPYVWGGTRPDQGFDCSGLVF
                     YAYNKILEAKLPRTANEMYHYRRATIVANNDLRRGDLLFFHIHSREIADHMGVYLGDG
                     QFIESPRTGETIRVSRLAEPFWQDHFLGARRILTEETIL"
BASE COUNT          281 a          272 c          269 g          266 t
ORIGIN      
        1 tttgttctcc ttaattatgt aaggagtatt tgatcatgca taagcattaa aaaacagttt
       61 caagtaatga ggaatatctc aaattttaaa cataaaatgc gaattattta gtacaaaaag
      121 tgtacttctg atgatctttc cctgggtgat ggaataacag gaaagcggct cgcaaaaacg
      181 cacagatttg taggcatgat aagacgcgtc agcgtcgcat caggcatctg cgcaccaccg
      241 ccggatgcgg cgtaaacgcc ttatccgtcc tacaaaatcg tctcttccgt caatatccgc
      301 cgcgcgccca aaaaatgatc ctgccagaaa ggttcagcta accgacttac ccgaatggtt
      361 tccccggtgc gcggtgactc aataaactga ccgtcgccca gatacacccc catatggtcg
      421 gcaatctcgc ggctgtggat atggaaaaac agcaaatctc cccggcgcag gtcgttattc
      481 gccacaatgg ttgcccgacg gtagtgatac atctcattgg ccgtacgcgg gagcttcgcc
      541 tcgaggatct tgttgtaggc ataaaaaact aacccactac aatcgaagcc ttgatcgggc
      601 cgcgtgccgc cccagacgta aggcttgcca agctgctgct ccagacggtg aatcgccact
      661 tcggtgatgt tatgcaggtg atcgctgctt aaaaagtggt tgttttccgc cagcgcctgc
      721 cagcgtctgt cactcgcctt gagcggcgca ggaaaccatt ctggatgctg ttttatctgc
      781 tcccggttgt gctggcgcag cgcccttctg ctttgggcat tgccttccac aatgtagctg
      841 gcctgttttt tcagatgggt ttggtactgt tttaataaac gcgcacggtt ttgcatccgc
      901 tggcgggcgg cgtagctgaa agagatatgt gatgtggtgg cgcaggcaaa tgaagaaaac
      961 ggcaagcaaa gcaacagtaa tcccgacaac acaaaagagg gaatcgacgg caaagacatt
     1021 ctcaataatg gctggtcatg cattgatgtt gcagtgtatc ctggcccgta cctgaacaaa
     1081 cttaataa
//
LOCUS       DAEEKQ010000404         1085 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.413, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000404 DAEEKQ010000000
VERSION     DAEEKQ010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.413"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..643)
                     /locus_tag="KLK57_004524"
     CDS             complement(<1..643)
                     /locus_tag="KLK57_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE0894680.1"
                     /translation="MSKISDLNYSQHITLADNFKQKSEVLNTWRVGMNNFARNAEGQD
                     NTRNILDPKTFLEFLVKIFTLGYVDFSKRSNEAGRNMMAHIESSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLSAFTTMGTRQGHTAIIFQQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNIPQELNNSQDLNNSQVSCKDSVDSTIT"
     gene            complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="KLK57_004525"
     CDS             complement(766..>1085)
                     /gene="tabA"
                     /locus_tag="KLK57_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HBE0894681.1"
                     /translation="YLISEDMTEPHEARRTEYHARYLDIQIVLKGQEGMTFSTQPAGT
                     PDTDWLADKDIAFLPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKM
                     LMA"
BASE COUNT          312 a          247 c          191 g          335 t
ORIGIN      
        1 ccgtaatcgt agaatcaact gaatctttac aactcacctg cgagttattc aagtcctgag
       61 aattatttaa ctcctgcgga atatttaata gacgagctat ttccttgtta gcttcgcgtt
      121 ggtaacatgc cagaatcttg ttagtaatca ggtgtaatga actctcatct tttctttcaa
      181 aggactttcc ttcataacga ttagtcgaac catcaggttg ttgaaatata atagccgtat
      241 gtccttgacg agtccccata gtagtgaaag cggaaagtgt gatttctatt tccaccttag
      301 ataaatccgc tcgttcccct tcaggattat tcataacaaa cttcattatc tcactgccat
      361 cattattttt gatataagat gaggactcaa tatgagccat catatttctt cccgcttcgt
      421 tggagcgttt gctaaaatcc acataaccta gagtaaatat ttttaccaaa aattccaaaa
      481 atgtcttagg atcaaggata tttcttgtgt tatcctgccc ctcggcatta cgggcaaaat
      541 tattcattcc aacacgccag gtatttaaaa cttcactttt ttgtttaaaa ttgtcggcta
      601 atgtaatgtg ttgagaataa ttcaaatctg atattttgct catgacgaac tccttgagtt
      661 aataagaacg tctaagcatt acaaatttta ctcacaaaaa aatatcaaaa aacgctcttt
      721 tcacaaactc gccgtaaacc tggagttatt tacggcgaaa agtcatcacg ccatcagcat
      781 cttcactact gctttgcgaa cctgggctgg tgcacccact gcgcacagcg gtttatgcac
      841 ttcccccgga taaaacacaa caaaatcacc ttcattaagg ataactgttt tctcatcaac
      901 gccttccggc aaaaatgcga tgtctttatc agctaaccag tcggtatccg gcgtgcctgc
      961 aggttgcgtg ctgaaggtca tgccttcctg accttttaac acaatctgaa tgtcgagata
     1021 gcgggcatgg tactccgtac ggcgagcttc gtgcggctcg gtcatatctt ccgagataag
     1081 ataaa
//
LOCUS       DAEEKQ010000405         1075 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.414, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000405 DAEEKQ010000000
VERSION     DAEEKQ010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1075
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.414"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            78..>1075
                     /locus_tag="KLK57_004526"
     CDS             78..>1075
                     /locus_tag="KLK57_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HBE0894682.1"
                     /translation="MSAYLRYIVQKADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPE
                     QRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEG
                     NRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRK
                     DIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTH
                     DRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFIT
                     SSWKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMTGLYNRKILTPELEQRL"
BASE COUNT          270 a          252 c          250 g          303 t
ORIGIN      
        1 ggcattttat cgccgcaagt gtcattgtgc tgacatcttc cttccttatt tttgaacttg
       61 tcgccagcga cagagcaatg agtgcctatc tgcgctatat cgtgcagaaa gcagactcct
      121 cctttcttta tgataagtat cagaatcaga gtattgccgc gcatgtgatg cgcgctctcg
      181 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
      241 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      301 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      361 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      421 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      481 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      541 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      601 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      661 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      721 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      781 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      841 acgtccatga cttaccgggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      901 tcacgtcttc gtggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      961 tggtccgaat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
     1021 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgca
//
LOCUS       DAEEKQ010000406         1051 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.415, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000406 DAEEKQ010000000
VERSION     DAEEKQ010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.415"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            32..943
                     /locus_tag="KLK57_004527"
     CDS             32..943
                     /locus_tag="KLK57_004527"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016238933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4329 domain-containing protein"
                     /protein_id="HBE0894683.1"
                     /translation="MKKEHADGSVTQSQFDAVGRLRTQTDAAGRTTEYSPDVVTGLIT
                     RITTPDGRASAFYYNHHSQLTSATGPDGLEIRREYDEWGNLLNEENPHQLQQLIRLPG
                     QQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNLYTYPLSPVNSMDPLGLYEFK
                     SKNIDDIGIFALAMCNGESINENKEYGGLICKKQGEYFPMNPISSNDNDSVDLRNIKC
                     PEGSERVGDYHTHGFYSDDKGNKVTKENDVYDSLNFSSKDLTNSYMNGMGKKEYSSYL
                     GTPNNTYLKYNPKAKGNGVTIIRQGSN"
BASE COUNT          338 a          192 c          270 g          251 t
ORIGIN      
        1 cacgtagggt gaagccgggc tgaagcgggt ggtgaaaaag gaacacgcgg acggcagcgt
       61 cacgcagagt cagtttgacg ccgtgggcag gctcaggaca cagacggatg ccgcaggccg
      121 gacaacagaa tacagcccgg atgtggtgac gggtctcatc acgcgcatca ccacgccgga
      181 tggcagggca tcggcgtttt actataacca ccacagccag ttaacgtcag ccaccgggcc
      241 tgacgggctg gaaatacgcc gggaatatga tgaatggggc aacctgctga atgaagagaa
      301 cccgcatcag ctgcagcagc ttattcgttt gccggggcag cagtatgatg aggagtccgg
      361 cctgtattat aaccgccacc gctattatga cccgctgcag gggcggtata tcactcagga
      421 tccgattggg ctgaaggggg gatggaacct gtatacatat ccgctgagcc cggtgaatag
      481 catggatcca ttaggattat atgaatttaa atcaaaaaat attgatgata ttggaatatt
      541 tgcattggca atgtgtaatg gagaatcaat taacgagaat aaagaatatg gtggactaat
      601 atgtaagaag caaggtgaat atttccccat gaatccgata agttcaaatg ataatgatag
      661 tgtagacttg cgaaatataa aatgccctga aggttcagag agagtaggcg attatcacac
      721 tcacggtttt tactctgacg ataaaggaaa taaagtaaca aaagaaaatg atgtttatga
      781 tagtctaaat ttttcaagca aagatttaac gaattcttat atgaatggaa tgggaaaaaa
      841 agaatacagt agttacttgg gaacaccaaa taacacctat ctaaaatata atcccaaagc
      901 taaagggaat ggagttacaa ttatcaggca agggagtaat taaaatgaag gcaatacaat
      961 gttgttattt ccttgcttta gtctccagtc atttaatggc tggagttatg ggtgatgata
     1021 atatattttt aagtaattcg gattcagctg c
//
LOCUS       DAEEKQ010000407         1049 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.416, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000407 DAEEKQ010000000
VERSION     DAEEKQ010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1049)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1049)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1049
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.416"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..679
                     /locus_tag="KLK57_004528"
     CDS             <1..679
                     /locus_tag="KLK57_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894684.1"
                     /translation="ITTHPSGIKDTLAWLVNASKLMTEFDDNGKIIFNINALKYTKAS
                     YFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLELAIGWRGALSESIKGVKFCIW
                     FSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGGVATAIGSLASFACVSFGFPVI
                     LVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDGLKRQQELDKWKRENMTPFMYV
                     LNTPPVI"
     gene            689..>1049
                     /locus_tag="KLK57_004529"
     CDS             689..>1049
                     /locus_tag="KLK57_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894685.1"
                     /translation="MRLHVKLKEFLSMFFMAILFFPAFNASLFFTGVKPLYSIIKCST
                     EIFYDWRMLILCFGFMSFSFLNIHVILLTIIKLFLIKKTKVVNFATDITIQLTLIFLL
                     IAIVIAPLIAPFVTGYVN"
BASE COUNT          327 a          174 c          191 g          357 t
ORIGIN      
        1 tatcactaca catccttcag gtataaaaga tacactcgcc tggttagtta acgcgagtaa
       61 attaatgact gagtttgatg acaatgggaa aattatattt aacattaatg ccttaaagta
      121 tacaaaagca agttactttg aaatacttgg agagaaatat ataaaaatca caacatccag
      181 cccctggctt ttagaaaagc tcggtaaata tattttctct tcacgagcgc ctcaagttct
      241 ggaactcgct attggctggc gaggagcttt atctgaatca ataaaaggag ttaagttctg
      301 tatctggttt tcagtagcct ggagaaccat agaatttatt atgagttcag agcgagatct
      361 ggttaatttt cttggcgatt tttcaatgga tgtggccaaa gcagttatag ccggtggtgt
      421 tgcaaccgct attggaagcc tggcttcttt tgcctgtgtt agctttggct ttccagtaat
      481 tcttgtcgga ggagcaattt tactgacagg gatagtgtgt acagttgttt taaatgaaat
      541 cgatgctcaa tgccatttat cagaaaaatt aaaatatgca attagagatg gactaaaacg
      601 gcaacaggaa cttgataaat ggaaaaggga aaacatgact ccatttatgt atgttcttaa
      661 cactccaccc gtgatatgag gattaaccat gcgtttacat gttaaattaa aagagtttct
      721 ttcgatgttt tttatggcca tattattttt ccctgcattc aatgcttctt tgttttttac
      781 cggagttaaa cctctatatt caataataaa atgctccacg gaaatatttt atgattggag
      841 aatgttgatt ctgtgctttg gtttcatgtc gttttccttt ttaaatattc atgtaatttt
      901 acttacgata ataaaattat tcttaatcaa aaaaacaaag gtagtcaatt tcgccacaga
      961 cataacaata cagctaactc ttatattttt actaatagct atcgtaatag cacctttaat
     1021 agctcctttt gtcacaggat atgtcaatg
//
LOCUS       DAEEKQ010000408         1048 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.417, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000408 DAEEKQ010000000
VERSION     DAEEKQ010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1048
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.417"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            82..396
                     /locus_tag="KLK57_004530"
     CDS             82..396
                     /locus_tag="KLK57_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001301570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894686.1"
                     /translation="MYNKKNKTGTVALSGTWQQGNRESKSIRRNIEYTWIENYDTAHL
                     TSKKVNKFEIMDQVDDDRLAQLIPDFYVFPEKSVSYNILKQGKHAFILSIGNRAIMHC
                     AR"
     gene            complement(430..510)
                     /locus_tag="KLK57_004531"
     CDS             complement(430..510)
                     /locus_tag="KLK57_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894687.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
     gene            complement(546..970)
                     /locus_tag="KLK57_004532"
                     /pseudo
     CDS             complement(546..970)
                     /locus_tag="KLK57_004532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417326.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          382 a          177 c          163 g          326 t
ORIGIN      
        1 aaaatgatat ataacgaaac atgcactgca aattgggtaa tttttaatga ccagggacga
       61 gcaaatctta ccatcgactt tatgtataac aaaaaaaata aaacaggaac tgttgcgttg
      121 agtggaacct ggcaacaagg taacagagaa agtaaatcaa tacggcgaaa tattgaatac
      181 acatggattg aaaactatga cacagcccat ttaacatcaa agaaagttaa taagtttgag
      241 attatggatc aagttgatga tgatagactt gcacaactta ttcctgattt ttatgtcttt
      301 ccagaaaaaa gtgtaagcta taatatacta aagcaaggta agcatgcttt tattttgagc
      361 attggtaaca gagcaataat gcattgtgca aggtaaacta aaatagataa caaaaaagat
      421 ggaacctcgt taatcataaa acaataaaat taaatattct cgcagtatat ggcagtctaa
      481 agcatcaaag ttttgatcaa catctttcat tttagacatc tccttgcaat gtaaaatatc
      541 atgtatcaac aacatctggt gcaaatccat gagtcgaact cgacattcat cttagctcga
      601 ttattattcc ttcgtacagt cgatgtaaac aatacagaaa gaggattatt aagaaccagt
      661 ttactcagga atcaatagag gaaaggacta acgtttcttt aaaagaattg atttcatcat
      721 ctgttaaact aaactcatca ttgacagatc gtgagatata actgttttta actttactct
      781 ttacgttgac tttattgaca gaattaacat tcacatatct tgaatttaat gtccatgatg
      841 ttgtttgcgt gagaacattc tcagcattaa ggaatttttt taacggaatc cttggtttct
      901 ttttcgagtc cgaatttaca atatcatgca tatagaccat ttcatgaatc tgcgaaaact
      961 caatgtccat tgtatacctc acatttttac cgtgactcga tgttactgtt caataatcac
     1021 cttccatcaa tactaaaatt aatacccc
//
LOCUS       DAEEKQ010000409         1042 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.418, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000409 DAEEKQ010000000
VERSION     DAEEKQ010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1042
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.418"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(89..>1042)
                     /locus_tag="KLK57_004533"
     CDS             complement(89..>1042)
                     /locus_tag="KLK57_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894688.1"
                     /translation="IAQQKAKTEKQNQRAQAAAARAQARQQQQIAARKEKAELKLLDV
                     SSSISAMHRLSVAEQYKAIAQAREIAKQYEQGAISLARMNSQMKRLQQQQRKINGNRK
                     AQFAPVKGGSGNSATMGALLFGGATAAAGVMAVSKTSEFMANSFANAETEGELIQRAR
                     LGGVDYNLLNNLERWAYDNGIDSLQGDPGRRKILDAIGKDVKERATKSYDEAEYVVDK
                     KTGKGIWKGGDSGINTLMNEGFLTKKDLKDFANNSAGLISKAVNGMVKKGYTDAQIGS
                     RLEDLADDLLLTSKYWQRSAREVQASITQQKAAGQWLTETQ"
BASE COUNT          223 a          255 c          209 g          355 t
ORIGIN      
        1 ctgtactcca atgactagtc taaaaactag tattaagact atcacttatt taagtgatac
       61 tggttgtctg gagattcagg gggccagtct actgtgtttc tgttagccat tgtccggcgg
      121 ctttttgctg ggtaatgctt gcctgcacct ctctggctga tctttgccaa tatttagaag
      181 ttaagaggag gtcgtccgct aaatcttcca gacgactacc gatctgtgcg tcggtatagc
      241 cctttttcac cattccgttg acagcttttg aaattaaacc agcagagtta ttggcaaagt
      301 ctttcaaatc ctttttagtt aagaatcctt cattcattaa agtgttaata ccagaatcac
      361 cgcccttcca tatgcctttc cctgttttct tatcaactac atattcagcc tcgtcatagc
      421 ttttggtagc gcgttccttt acatctttac cgatggcatc tagtattttt ctgcgtcccg
      481 gatcaccctg gaggctatcg atcccgttgt catacgccca acgctcaaga ttattaagta
      541 agttgtaatc aactccacca agccttgcac gttggattag ctccccttct gtttcagcat
      601 tagcaaagct gttagccata aattcagagg ttttactaac agccatcacg ccagcagcag
      661 ccgtagcgcc tccaaataac agagcgccca tcgtggcgga attccctgat cctcccttaa
      721 cgggggcaaa ttgagctttt ctgttgccat taatcttacg ttgttgctgt tgtaggcgtt
      781 tcatctgact attcatacgc gccaggctga tagccccttg ctcatattgt tttgctatct
      841 ctctagcctg tgcaatagct ttgtattgtt ccgctacaga gagacggtgc attgcactga
      901 tagagcttga tacatcaagg agttttaatt ctgctttctc tttacgtgct gctatttgtt
      961 gctgttgcct tgcctgtgct cttgctgcgg cagcttgtgc tctttgattc tgcttttctg
     1021 ttttagcttt ctgttgagca at
//
LOCUS       DAEEKQ010000410         1037 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.419, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000410 DAEEKQ010000000
VERSION     DAEEKQ010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1037
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.419"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..906)
                     /locus_tag="KLK57_004534"
     CDS             complement(<1..906)
                     /locus_tag="KLK57_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter YfaL"
                     /protein_id="HBE0894689.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSIADGQWLVFSDMTNNASGGAVFLQQGAEFTLSPENETGMTLFAN
                     NTVTGEYYNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLSVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPPGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDI"
BASE COUNT          293 a          236 c          207 g          301 t
ORIGIN      
        1 aatatcaaag gtaacttcac ttaagccgag atacataaag ccacccgccg cagtggaagg
       61 accatctcca tagcctgctg cgctgttgtt ctcatccact aacacgccgc cgttctggct
      121 gtagctgtca tcaacagaaa tatcaataag atagggagcc gtcgcgctat tggtatatat
      181 cgccccgcca taaccttcgg cagtgttatt tgtaaaggca gtgttattta ttatcgtata
      241 acctggagga tgcttgctgt cgctattatt atcggtaaca tcgattgccc cgccatcgcc
      301 atcactgtaa cttgttgatg tatatgcctg gttgttatca aaaataacat cacttaaata
      361 gacgtcatta ttaatggtat aaattgcgcc acctttgcca tcattagcga tattattgcg
      421 aaacatggcg ttagtgacac ttaaatcgac ggcaccagta tcgttagtac cagaagaata
      481 gattgcgcca ccatagccgc ctgcgacgtt accggaaaaa atgacatcag taagattcag
      541 cgttgagttt tctttagcaa atatggcccc gccgttataa tattctcctg taacggtgtt
      601 attggcaaac agagtcattc cagtttcgtt ttctggtgat aatgtaaatt ccgctccttg
      661 ttgcaaaaat acggccccac cgctggcatt attggtcata tccgaaaaaa ccagccattg
      721 cccatcggcg atactccagt cctgcgtaat gcctgaaagg ctttgcctgc tggcgtgaca
      781 actcgctttg atatcatatc cctggcataa atcaatggcc gccgcgacac cgttagcaga
      841 gaaaagagat gcaatcattg acgggagtag agataaatac tccttgcgta gaaagataat
      901 ccgcatatta atctgaccat cattttatat aagacggcat atttaaacgc attatacaat
      961 atgggttatc tttcttttgt aaattgtcca acccgatgca atggctattt tgttattatt
     1021 tgatgaataa tatcagt
//
LOCUS       DAEEKQ010000411         1023 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.420, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000411 DAEEKQ010000000
VERSION     DAEEKQ010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.420"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(77..865)
                     /locus_tag="KLK57_004535"
     CDS             complement(77..865)
                     /locus_tag="KLK57_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HBE0894690.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(905..>1023)
                     /locus_tag="KLK57_004536"
     CDS             complement(905..>1023)
                     /locus_tag="KLK57_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HBE0894691.1"
                     /translation="KEAIRFASAVAAMKCTQPGGRAGIPNREQTESFLSLYA"
BASE COUNT          255 a          263 c          239 g          266 t
ORIGIN      
        1 atcgctggca ggtttttttt gcttatggaa aacaaaatcc gctgaaattc ccagcggatt
       61 ttccgaaatt tcaatttcag gatacttctt gctcggcaac caccagttca acattctctt
      121 cacttaacaa ctggcagaaa gaattgggag gcggggcatc agtaaaaaaa gccctgacat
      181 tccgtgcatt gccaattgaa acggcagcag acgcggcaaa cttcgtatga tcggccacta
      241 acaatgtatt ccgcgcatgt ttaatcatcg ttctcgcgac taacgcttca tttacatcaa
      301 attccagtag ggtgccgtca tgttcaatgg cacctatact ggtgattaaa taatctgcgc
      361 ggaagccttc aataaaatcc acggctcctg ggccgataat cccgccgtta tgagcgcgta
      421 aagtgcctcc cggcaccatc acttcaatat cctgattctt ataaagaatc tgtgccacac
      481 gtaggctgtt agtgataatg cgtaaatcac gccggttgag taatgccctg gcaacggctt
      541 ctacggttgt accgatggtg ataaagacgg tacagcgttc gggaaggtaa tcggcgactg
      601 cctcagcgat cgcccgtttc tcggcggtga gcgaaagttc ccgttgctca aaagcggtat
      661 tcatgacgct ggagacgcgc ccggcccctc catgatgacg ggtgatcaag ccctgctcac
      721 tgagttttcg gatatcccgg cgcacggttt gcgttgaaac atccagcagt tgtgccagtt
      781 cttcaatatt catataaccg cgttctgcga ttagatgaac gagttggtca tggcgtggat
      841 tacctgtcac ttcgatgata ctcatgaata ctcccttgcc cctcctcggt agatgtttac
      901 cattttacgc ataaagtgac aaaaatgatt cggtttgctc acgattagga atgccggccc
      961 gaccgccagg ctgggtacat ttcatcgcgg caaccgcact ggcaaaacga atagcctctt
     1021 ttg
//
LOCUS       DAEEKQ010000412         1013 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.421, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000412 DAEEKQ010000000
VERSION     DAEEKQ010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1013
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.421"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            44..420
                     /locus_tag="KLK57_004537"
                     /pseudo
     CDS             44..420
                     /locus_tag="KLK57_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000937824.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            427..819
                     /locus_tag="KLK57_004538"
     CDS             427..819
                     /locus_tag="KLK57_004538"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894692.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGNSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSEKARN
                     ASAMLQRGLKAVKLSWQNHLQQVPIIRL"
     gene            777..>1013
                     /locus_tag="KLK57_004539"
     CDS             777..>1013
                     /locus_tag="KLK57_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894693.1"
                     /translation="MAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDD
                     AKFRRDNVKEIISNLFANKVDVDHPENKYKDFKDL"
BASE COUNT          393 a          176 c          175 g          269 t
ORIGIN      
        1 gtaatatata tttgtaacaa atcaatcaaa atggaataaa atcatgctac catctatttc
       61 aatcaacaat accagcgcaa cttacccaga atccattaat gaaaataaca atgatgaagt
      121 taatggatta gtacaagagc tcaaaaacct ttttaatggt aaggaaggaa taagcacctg
      181 tattaaacat ctacttgagc ttataaaaaa cgccatacga gtaaacgacg atccttaaga
      241 tttaatatta ataattcctc agttacttat attgatattg gctccaatga tacagaccat
      301 attactattg gtatcgacaa ccaagaacca atagaattac ctgcgaacta taaagacaaa
      361 gaactcgtcc gtactatcat taatgacaac atagttgaga agactcatga tatcaataac
      421 aaggaaatga tcttcagcgc attaaaagaa atatatgatg gagatcctgg ttttatcttc
      481 gataagatat cacacaaact cagacatacg gtaacagaat ttgatgagag cgggaatagc
      541 gaaccaacgg acttatttac ctggtacggt aaagataaaa aaggcaactc tctcgctatt
      601 gtaattaaaa ataaaaacgg aaatgattac ttatctctcg gttactacga tcaggacgac
      661 taccacattc aaagaggaat tcgtattaat ggtgatagtc tcacccaata ttgtagtgaa
      721 aaagccagga atgcttcagc aatgcttcag cgtggtttga aagcagtaaa gctatcatgg
      781 cagaatcatt tgcaacaggt tccgatcatc aggttgtaaa cgagctcaac ggggaaagac
      841 tgagagaacc taacgaagtt tttaaacgtt taggtcgagc aataagatat aattttcaag
      901 tggacgatgc aaaatttaga cgcgacaatg taaaagaaat aatttctaat ttattcgcta
      961 acaaagttga cgttgaccat cctgaaaata aatacaaaga ctttaaggat ctc
//
LOCUS       DAEEKQ010000413         1003 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.422, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000413 DAEEKQ010000000
VERSION     DAEEKQ010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1003
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.422"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>1003
                     /locus_tag="KLK57_004540"
     CDS             <1..>1003
                     /locus_tag="KLK57_004540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="inverse autotransporter adhesin-like protein
                     YeeJ"
                     /protein_id="HBE0894694.1"
                     /translation="SGNLIEGLTVYFALKSGSATLTSLTAVTDQNGIATTSVKGAMTG
                     SVTVSAVTTAGGMQTVDITLVAGPADASKSVLKNNRSSLKGDFTDSAELHLVLHDISG
                     NPIKVSEGLEFVQSGTNVPYVQVSAIDYSKNFSGEYKATVTGGGEGITTLIPVLNGVH
                     QAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGAYYQLNNDNFAPGKTAA
                     DYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSYVYEIRVKSWWVNAGEA
                     FMIYSLAENFCSSNGYTLPRANYLNHSSSRGIGSLYSEWGDMGHYTTEAGFQSNMYWS
                     "
BASE COUNT          293 a          223 c          254 g          233 t
ORIGIN      
        1 gcagtggtaa cctgatcgaa ggtctcactg tgtactttgc cttaaaaagc ggctctgcca
       61 cattaacgtc attaacagcg gtgaccgatc aaaacggaat cgcgacaaca agcgtgaaag
      121 gagcgatgac aggtagcgtc acggtaagcg cagtcacgac cgctggtgga atgcaaacag
      181 tagatataac gctggtggca ggcccagcag acgcctcaaa gtcagtcctt aagaacaatc
      241 ggtcatcatt gaaaggagac tttaccgaca gtgctgagct acatcttgtt ctgcacgata
      301 tatcaggcaa tccgatcaaa gtttctgaag ggctggaatt tgtacagtca ggtaccaacg
      361 tgccctatgt gcaagttagt gcaattgact acagtaaaaa tttctcaggc gagtacaaag
      421 ccactgttac aggaggcgga gagggtatca caacgctgat ccctgtattg aatggtgttc
      481 atcaagctgg tctgagtacc acaatacaat tcactcgcgc agaagacaaa ataatgagcg
      541 gtacagtatc agtcaatggt actgacctac cgacaactac attcccttcg caggggttca
      601 ccggggcgta ttatcagttg aataatgaca actttgcccc aggaaaaacg gcggctgatt
      661 atgagttttc aagctctgcc tcctgggtcg atgttgatgc taccggtaaa gtgacattta
      721 aaaatgtcgg cagcaattcg gaaaggatta cggcgacacc aaaatcagga ggccctagct
      781 atgtatacga aatccgtgtg aagagttggt gggtgaacgc cggcgaggct ttcatgatat
      841 acagccttgc tgaaaatttt tgcagcagca atggctacac gctccccaga gcaaactatt
      901 taaaccacag tagttcccga ggcatcgggt cactgtacag tgaatgggga gatatggggc
      961 attacacgac tgaagctggt tttcaatcaa atatgtattg gtc
//
LOCUS       DAEEKQ010000414          996 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.423, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000414 DAEEKQ010000000
VERSION     DAEEKQ010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.423"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            14..>996
                     /gene="gspA"
                     /locus_tag="KLK57_004541"
     CDS             14..>996
                     /gene="gspA"
                     /locus_tag="KLK57_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspA"
                     /protein_id="HBE0894695.1"
                     /translation="MSTRREVILSWLREKRQTWRLCYLLGEAGSGKTWLAQQLQKDKH
                     RRVITLSLVVSWQGKAAWIVTDDNAAEQGCRDSAWTRDEMAGQLLHALHRTDSRCPLI
                     IIENAHLNHRRILDDLQRAISLIPDGQFLLIGRPDRKVERDFKKQDIELVSTGRLTEH
                     ELKASILEGQNIDQPDLLLTSRVLKRIALLCRGDRRKLALAGETIRLLQQAEQTSVFT
                     AKQWRMIYRILGDNRPRKMQLAVVMSGTIIAITCGWLLLSSFTAPFPVPAWLVPVTPV
                     VKQDMTKDIAHVVMRDSEALSVLYGVWGYEVPADSAWCDQAVRAGLACKSG"
BASE COUNT          251 a          230 c          269 g          246 t
ORIGIN      
        1 aacaggaagt tctatgtcta cgagaagaga agttattctt tcctggttgc gtgagaaacg
       61 tcagacctgg cgtctgtgct atttgttggg tgaggctgga agtggaaaaa cctggctggc
      121 gcagcaactg caaaaagata aacatcgccg tgtgattact ttaagcctcg ttgtttcctg
      181 gcaaggtaag gccgcatgga tcgttaccga cgataacgcg gctgaacagg gctgccgtga
      241 cagcgcctgg acgcgagatg agatggcggg gcaattactg catgcgcttc atcgaactga
      301 tagtcgctgc cctcttatta ttattgaaaa tgctcacctg aatcaccgcc gcatacttga
      361 tgatctccaa cgcgcgatat ctcttattcc tgacggtcaa tttttgctga tcggcagacc
      421 cgatcgaaaa gtcgaacgtg attttaaaaa acaggacatt gaacttgtct cgacaggacg
      481 tctgacggag cacgaactta aggcaagcat ccttgaaggt cagaatattg accaacccga
      541 tctcctttta acctccagag ttctgaaacg gatagcttta ttatgtcggg gcgatcgcag
      601 aaagctggcg cttgccggcg aaacaattcg cttattacag caagccgagc agaccagcgt
      661 tttcactgca aaacaatggc gaatgattta ccgcatattg ggtgataacc gcccccggaa
      721 aatgcagctt gccgtggtaa tgagtggaac cattatcgct attacctgtg gttggctact
      781 gctgtcctct ttcacagctc cgttccccgt tcctgcgtgg ctggtacccg taacgccagt
      841 agttaaacaa gatatgacta aggatattgc tcatgtggtg atgcgcgata gtgaggcgct
      901 aagcgtgtta tacggcgtat gggggtacga ggtcccagct gattcggcct ggtgcgatca
      961 ggcagtaaga gcggggctgg catgtaaaag tgggaa
//
LOCUS       DAEEKQ010000415          990 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.424, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000415 DAEEKQ010000000
VERSION     DAEEKQ010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..990
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.424"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            89..>990
                     /gene="istA"
                     /locus_tag="KLK57_004542"
     CDS             89..>990
                     /gene="istA"
                     /locus_tag="KLK57_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISSso4 family transposase"
                     /protein_id="HBE0894696.1"
                     /translation="METCFKILQLKFDKKLTNRCIGLTLHISASTVFEVLARFKASSL
                     SWPLPADISHDTLEKLIFPPKDTSASELVMPDMLYFDTEMRKPGVTRQLLWMEYKAQA
                     GDKAMGYSHFCRCYRKWKKTRRLSMRQEHRAGEKLFIDFCGPTVPVINPDTGEIRRVA
                     IFVAVMGASNYTYVEACEGQDMMSWLNAHSRCLTFLGGVPKLLIPDNLRSAVKKADRY
                     EPVINDSYQALAEHYGTVIIPARPRKPKDKPKAENGVLIVERWLLARIRNETFHTLRA
                     LNARLRELLTDMNNRPMKGYGNQTR"
BASE COUNT          258 a          267 c          243 g          222 t
ORIGIN      
        1 ggaattaact cttttcacca cagtctccat tgccgtgttt aaaccagtgg agacgtcaat
       61 gtccaaaaag agaaaatcca ggagcactat ggaaacttgt tttaagatcc ttcagctcaa
      121 gttcgataag aagctgacta accgttgtat cggactgacg ttacacatca gcgcctcaac
      181 cgtttttgag gttcttgccc gcttcaaagc ctcttctctg tcatggccat tacctgcgga
      241 tatatcccat gacacgctgg agaaactcat tttcccgccc aaagacacct ctgcgtcaga
      301 gcttgttatg cctgacatgc tgtacttcga tacggaaatg agaaaacctg gcgtgacgcg
      361 ccaactgttg tggatggaat acaaggcaca ggcgggtgac aaagcgatgg gatactcaca
      421 cttctgccgt tgctaccgga agtggaaaaa aacgcgacgc ctctccatgc gacaggagca
      481 tcgtgctggc gaaaagctct ttatcgactt ctgtggtcct acggtccctg ttattaatcc
      541 ggacaccgga gagatacgcc gcgtggcaat cttcgtggct gtaatggggg cgtccaacta
      601 tacctatgtt gaagcgtgcg aaggtcagga catgatgtca tggttaaatg cccacagccg
      661 ttgcctgacc ttcctgggcg gcgtaccgaa gctgctcatc cccgataacc tcagaagcgc
      721 agtgaaaaaa gccgatcgct atgagcctgt catcaacgac agttatcagg cgctggctga
      781 gcactatggc acggttatta tcccggcccg cccgcgcaaa cctaaagata aaccaaaagc
      841 agaaaacggc gtcctcatcg tcgagcgatg gcttcttgcc cgcatccgta atgaaacctt
      901 ccacacgctc agggcgctga atgcacgcct tcgggagttg ctgacggaca tgaataaccg
      961 accaatgaag ggatacggta atcaaacccg
//
LOCUS       DAEEKQ010000416          976 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.425, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000416 DAEEKQ010000000
VERSION     DAEEKQ010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.425"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>976
                     /locus_tag="KLK57_004543"
     CDS             <1..>976
                     /locus_tag="KLK57_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBE0894697.1"
                     /translation="DLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQWQYDDH
                     GWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTVETPETGEMLWEHETGHAYSEQGLA
                     TRQEPDGLPPVEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETARSFGGEAYELATAW
                     NTSGQLRSRHLNLPQLDRDYDWNDNGQLIRISGPQESREYRYSDTGRLTGVHTTAANL
                     DIDIPYATDPAGNRLPDPELHPDSTLTAWPDNRIAEDAHYVYRYDEYGRLAEKTDRIP
                     EGVIRMHDERTHHYHYDSQHRLVFYTRIQHGEPQVESRYLYDPLGRRTG"
BASE COUNT          222 a          291 c          331 g          132 t
ORIGIN      
        1 atgacctgac cggaaaactc acgcagagtg aagatgaggg gcttatcacc ctctggcatt
       61 acgatgcgtc ggaccgtatc acccggcgca cggtgaacgg tgaaccggcg gagcagtggc
      121 agtatgacga ccacggctgg ctgacagaaa tcagccacct gagtgaaggc caccgtgtgg
      181 cggtccacta cggctatgac gataaaggcc gtctgacggg cgaacggcag acggtggaga
      241 ccccggagac gggggagatg ctgtgggagc atgagacggg gcacgcgtac agcgaacagg
      301 ggctggcgac ccgtcaggag ccggacggcc tgccgccggt ggagtggctg acgtatggca
      361 gcggttatct tgcggggatg aagctgggcg gaacaccgct ggtcgagtac acgcgggacc
      421 ggctgcaccg tgagacggcc cgcagcttcg gcggggaggc atatgaactt gccaccgcct
      481 ggaataccag cggccagctc cggagcaggc acctgaacct tccgcagctt gaccgtgact
      541 acgactggaa cgacaacgga cagctaatcc gcatcagtgg cccgcaggag agccgggagt
      601 accgttacag cgacacgggg aggctgacgg gcgtccacac cacggcagcg aacctggata
      661 tcgatatccc gtatgcaaca gacccggcag gaaatcggct gccggacccg gaactgcacc
      721 cggacagcac cctcacggcg tggccggata accgcatcgc ggaagatgcg cactatgtct
      781 atcgctacga tgaatacggc aggctggcgg agaagacgga ccgtatcccg gaaggggtta
      841 tccggatgca cgacgagcgc acccaccact atcactacga cagccagcac cgcctggtgt
      901 tctacacgcg gatacagcac ggcgaaccac aggtggagag ccggtacctc tatgacccgc
      961 tgggccgccg gacggg
//
LOCUS       DAEEKQ010000417          971 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.426, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000417 DAEEKQ010000000
VERSION     DAEEKQ010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 971)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..971
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.426"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..133)
                     /locus_tag="KLK57_004544"
     CDS             complement(<1..133)
                     /locus_tag="KLK57_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HBE0894698.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALARQNENHE
                     "
     gene            141..572
                     /gene="tnpA"
                     /locus_tag="KLK57_004545"
                     /pseudo
     CDS             141..572
                     /gene="tnpA"
                     /locus_tag="KLK57_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          297 a          222 c          184 g          268 t
ORIGIN      
        1 cctcatgatt ctcattctga cgtgccagag cacgattgaa aacgaaacga catgcgcctg
       61 cgaagcgcct catttcacgc tcctgttgac caccgggtct taactggaat ttaaatgctt
      121 gcagtcgttt cacaggccaa atgatacttt ggtctgtgaa aaaagaaacc gatatccggc
      181 gtggaaggca ttgtgttttc ctgatgcatg ttcacctggt ctttgtcacc aaataccgac
      241 gtcagatttt tgaccatgac gcgacagaaa aactacgcac ttacttttca aatgtatgtg
      301 ctgattttga agctgaactg gttgaaatgg atggcgaacc agatcacgtc catttgttaa
      361 ttaactatcc tcccaaactg gcgatatcca gtctggtaaa cagcctcaaa ggcgtatcgg
      421 gtaggtgact gcgacgagat cgaccagata ttgcagtcag gtattactac aaaggcgttt
      481 tgtggagtgt tggctatttt gccagtagct gcggaggtgc gccaatatcc gtcatccgcc
      541 aatacattga acaacagcaa acacctggtt aggtggaaaa ccacgcctta tatccctgcc
      601 ctgaaggacg gggttttacg gcgcaccgga taagcctgga gattatctac accagcatgg
      661 acatcattgt actatgcccc gtacatgccc catcacatcc tcaacatcaa agtattagag
      721 ccttgcaaac cattatgcgt ctcagtttgt ctcatctatc ttacgcaaaa aaaaatagaa
      781 ctaatatcaa gcggcgctat attgcgtatt aaataaaacc acctcaatta cattaataaa
      841 tctaaacaaa catcatgcaa ataatttcca tggttatttc taggctattt aaattaaata
      901 aataaattat gagtcaatca taaataacaa cctttaatat aatttaacta ccaaatttat
      961 attcgttcac a
//
LOCUS       DAEEKQ010000418          963 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.427, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000418 DAEEKQ010000000
VERSION     DAEEKQ010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.427"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>963
                     /locus_tag="KLK57_004546"
     CDS             <1..>963
                     /locus_tag="KLK57_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="cleaves origin of transfer and unwinds; oriT
                     relaxase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
                     /protein_id="HBE0894699.1"
                     /translation="VALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDRE
                     VMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRN
                     GKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVR
                     IARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQR
                     QAEEAIRRETERRADEIVRKMAENKPDLPDGKTEQAVREIAGQERDRADITEREAALP
                     ESVLRESQREQEAVREVARENLLQERLQQIERDMVRDLQKEKTL"
BASE COUNT          220 a          239 c          350 g          154 t
ORIGIN      
        1 acgtggccct gtcgcgtatg aagcagcatg tgcaggtgta caccgataac cgtcagggct
       61 ggacggatgc cattaacaat gccgtacaga aaggaactgc ccacgatgtg ctggagccga
      121 aaccggaccg ggaggtcatg aatgcgcagc ggctgttcag tacggcacgg gaactgcggg
      181 acgtggcggc aggccgtgcc gttctccgtc aggcagggct ggccggggga gacagccctg
      241 cacggtttat tgcaccggga cgtaaatatc cgcagccgta tgtggcactg ccggcgtttg
      301 accgtaacgg caagtccgcc ggtatctggc tgaacccact gaccacggat gacggaaacg
      361 ggctgcgggg attcagcggt gaaggacggg tgaaaggcag cggggatgcg cagttcgtgg
      421 ccctgcaggg tagccgtaac ggagagagcc tgctggctga taatatgcag gatggtgtcc
      481 ggattgcccg tgataatcct gacagcggtg tggtggtaag aattgccggt gaaggtcgtc
      541 cgtggaatcc cggtgccatc accggtggtc gcgtgtgggg ggatatcccg gacagcagcg
      601 tccagccggg agccggaaat ggcgaaccgg tcacggcaga ggtgctggca cagcggcagg
      661 ctgaagaggc catccgccgt gaaacggaac gccgtgcaga tgaaattgtc cgtaaaatgg
      721 cagagaacaa acctgacctg ccggatggca aaacagagca ggctgtcagg gaaattgccg
      781 ggcaggagcg tgaccgggct gacataactg aacgggaagc cgcgctgccg gagagtgtgc
      841 tgcgtgaatc acaacgggag caggaagcgg tccgggaggt tgcccgggaa aatctgctgc
      901 aggagcgtct gcagcagata gagcgggata tggttcgtga cctgcagaaa gagaaaaccc
      961 tgg
//
LOCUS       DAEEKQ010000419          962 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.428, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000419 DAEEKQ010000000
VERSION     DAEEKQ010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 962)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..962
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.428"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..400
                     /locus_tag="KLK57_004547"
     CDS             <1..400
                     /locus_tag="KLK57_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tail tape measure protein"
                     /protein_id="HBE0894700.1"
                     /translation="DVINAIVKPFYERQMSKSDNPIDKVVRSKVEPTDQALTGDMNSL
                     AAVLWAMVQGKSKDVPQFSPEALNNLKGRVETFTQDLKVIKPSVNIQPQTINITTQTV
                     LDGKVIDERTTSHINRMQEDTLISSAYPEE"
     gene            574..>962
                     /locus_tag="KLK57_004548"
     CDS             574..>962
                     /locus_tag="KLK57_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894701.1"
                     /translation="MFDTKQIRYSGKDGIYFHLRDNVESFLALTATEQMDYDSPMMVT
                     TQAMQSGQTITDNVQRAPKTISISGVVVVDSTGSVLLTRQGQLVETFISTVEQWRDQK
                     QIITIVCRDGIAITDSIITQFKASKEHT"
BASE COUNT          328 a          177 c          190 g          267 t
ORIGIN      
        1 tgatgtgatt aatgccattg tcaaaccgtt ctatgaacgt cagatgagta agtcagataa
       61 tccaattgat aaagttgtaa ggtcaaaggt ggaacccaca gaccaagcat tgactggtga
      121 catgaactct ttagccgctg ttttatgggc tatggttcag ggcaaatcaa aagatgttcc
      181 acagttttca cctgaagcat taaataatct gaaaggtcga gtggaaacat tcacacaaga
      241 tttaaaggtt atcaaaccgt cagtaaatat tcagcctcag acaatcaata tcacaactca
      301 gacggtactt gatggaaagg ttattgatga aagaaccaca tcgcatatta atcgtatgca
      361 ggaagatacc ttgattagct cagcttatcc agaggaataa acaatgttag agctactaat
      421 atttattgcc acacatggca atctattaat ctttatctag tatgacgatt caactatgaa
      481 tagtcgaatg ctacgtgaaa tcttctgaac agataccaac atcaaaaatt agatttcacg
      541 tagctcacgc ttaacagaat aggaataaca acaatgtttg acacaaagca aattagatac
      601 tcaggtaaag atggtatcta ttttcacctt agagataatg tggaatcttt tcttgcctta
      661 acagctactg aacagatgga ctatgactca ccgatgatgg tgacaactca ggctatgcaa
      721 tcggggcaaa ctatcactga taatgttcag agagcaccta agaccatctc tattagcggt
      781 gttgtagtgg ttgattcgac gggttcagtt ctattaacca gacaggggca acttgtagaa
      841 acctttattt ctacggttga acaatggcga gaccagaagc aaatcatcac tattgtttgt
      901 agagatggaa tagctatcac tgatagtatc attactcagt ttaaagcgag taaagagcat
      961 ac
//
LOCUS       DAEEKQ010000420          960 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.429, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000420 DAEEKQ010000000
VERSION     DAEEKQ010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 960)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..960
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.429"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>960
                     /locus_tag="KLK57_004549"
     CDS             <1..>960
                     /locus_tag="KLK57_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBE0894702.1"
                     /translation="AAVQTLREMNADNLRKVPADAPTAFIKPRWKPLVITPEGLDRKF
                     YEICALSELKNALRSGDIWVKGSRQFRDFDDYLLPAEKFAALKREQALPLAINPNSDQ
                     YLEERLQLLDEQLATVTRLAKDNELPDAILTESGLKITPLDAAVPDRAQALIDQTSQL
                     LPRIKITELLMDVDDWTGFSRHFTHLKDGAEAKDRTLLLSAILGDAINLGLTKMAESS
                     PGLTYAKLSWLQAWHIRDETYSAALAELVNHQYRHAFAAHWGDGTTSSSDGQRFRAGG
                     RGESTGHVNPKYGSEPGRLFYTHISDQYAPFSTRVVNVGVRDST"
BASE COUNT          189 a          335 c          291 g          145 t
ORIGIN      
        1 ggcagccgtg cagaccctgc gtgagatgaa cgccgacaac ctgcgcaagg tgccggccga
       61 tgcacccacg gccttcatca agccgcgctg gaagccgctg gtgatcaccc cggaaggcct
      121 cgaccggaaa ttctacgaaa tctgcgccct gtccgagctg aagaacgccc tgcgctccgg
      181 cgacatctgg gtcaagggct cgcggcagtt ccgcgacttc gacgactacc tgctgccggc
      241 cgagaagttc gccgcactca agcgcgagca ggccctgccc ctggcgatca acccgaacag
      301 cgaccagtac ctggaagagc gtttgcagct gctggacgag cagttggcca ccgtcacccg
      361 cctggccaag gacaacgagc tgcccgatgc catcctcacc gagtcagggc tgaaaatcac
      421 cccgctggat gcggcggtgc cggatcgggc gcaggcgctg atcgaccaaa ccagccagtt
      481 actgccgcgc atcaagatca ccgaactgct gatggacgtg gacgactgga cgggcttcag
      541 ccgccacttc acccacttga aggacggggc cgaggccaaa gacaggacgt tgctgctgtc
      601 cgcaatcctc ggtgatgcga tcaacctcgg gctgaccaag atggccgagt cgagccccgg
      661 cctgacctac gccaagctgt cctggctgca agcctggcac atccgcgacg aaacctattc
      721 ggcggccttg gccgagctgg tcaaccacca gtatcgccac gcctttgccg cccactgggg
      781 cgacggcacg acctcatcct ccgatggcca gcgcttccgc gcgggtggcc ggggcgagag
      841 caccgggcac gtcaacccga agtacggtag cgagccggga cggctgttct atacccatat
      901 ctccgaccag tacgcgccgt tcagcacccg cgtggtgaat gtcggcgtcc gcgattccac
//
LOCUS       DAEEKQ010000421          959 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.430, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000421 DAEEKQ010000000
VERSION     DAEEKQ010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.430"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..228)
                     /locus_tag="KLK57_004550"
     CDS             complement(<1..228)
                     /locus_tag="KLK57_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3289 family protein"
                     /protein_id="HBE0894703.1"
                     /translation="MNNILLPPINIPCTLFETISLFDDFSADDMQYGDMVEQDFLSLG
                     LSDISAKVDPYRLIKYHFPGPGSINVAFSASS"
     gene            385..561
                     /locus_tag="KLK57_004551"
     CDS             385..561
                     /locus_tag="KLK57_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001351582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HBE0894704.1"
                     /translation="MLNLPIRKLFPTRIASSYGMNFEFMPELKWSFGYPGAIIFMILA
                     GLTPYLYFKRKNWL"
BASE COUNT          313 a          165 c          186 g          295 t
ORIGIN      
        1 acttgaggca ctaaaagcaa cattaataga acctgggcca gggaaatggt atttaataag
       61 tctgtaagga tcaacttttg ctgagatatc gcttaggccg agtgagagaa agtcttgttc
      121 taccatatca ccatattgca tatcatcagc agaaaaatca tcgaataaac taatagtttc
      181 gaaaagtgta cagggaatat taattggcgg aagtaatatg ttattcattt gcatttgcct
      241 tggttatatt taagctcaag atcaattcga gaacaaatgc tagggtaaaa aaatatttaa
      301 aaaagaagta cacttcacat tttttcatta catctctgaa gagaacctcg ttaatgattg
      361 gtcttgctca ttgttaataa caaaatgttg aatttaccga ttaggaaact ttttcctacg
      421 cggattgcct ccagctatgg catgaacttt gagtttatgc cagaactgaa gtggagcttt
      481 ggctaccctg gcgcgattat ctttatgatc cttgcgggcc tgacaccgta tctgtacttt
      541 aagcgtaaga actggctgta actatacgat gtcgctgtga gtaagttagt gtgctgacag
      601 cgacatttta gccgagggaa tcgcagataa cataattgtt gtgaataata tccatgccac
      661 gttgagttgg acgcttaatg actcacatct caccccttca attgctgcag agataataga
      721 aaaaagtgtc aaacccaatg aaatcaatac aaagtagatt gccttacatc cagtatctct
      781 tattcttttt gctccaatat tgatatttac ataggtatta ttaataacca gaataaaact
      841 caaagccagg ttgatatcat tcaaagttat taatattgac catgatgtta atgtatagat
      901 tatgaactga aagcgtgata aacgctcaag cctggatggg tagaatatat ttctgatgg
//
LOCUS       DAEEKQ010000422          958 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.431, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000422 DAEEKQ010000000
VERSION     DAEEKQ010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..958
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.431"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(171..842)
                     /gene="queE"
                     /locus_tag="KLK57_004552"
     CDS             complement(171..842)
                     /gene="queE"
                     /locus_tag="KLK57_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HBE0894705.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVISRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          258 a          235 c          211 g          254 t
ORIGIN      
        1 accgcaataa ttttcactcc agcgaaaaat taatgccaca gaatttgtag agaatgctaa
       61 tgattagccc tggatgggta aaatggagga gttttcagag gcgacaaaca atataaatga
      121 gtagaagtct ttacaggttc cacccattta taacgcttat aaatgtttaa tcaggcaata
      181 tttagatatt tatgtgtttg catcgacaaa cgccaattac gcgcaatgca ggtttcaatg
      241 cacaaacgtg tggcatcatc cttttggcta atcggctgca gtgcaatgac tcgcggttta
      301 tcatcggtca gcgttgccaa tagttcatcc agtgcttcaa tatcgcgtac gcgccccact
      361 ggatgcttga tttcgttggc tcgctccagt gcctgtgaca acacgtcata gccgccgcgc
      421 atgttcagct ttggcgatac ggtaacccag gtattcggtg tgcagcgtac ctcatgagta
      481 ccactggttt cgatctggca gctaaaaccg ttcttttcga gcagatcagt cagtggtagc
      541 aaatcatgaa tgcaaggctc accacccgta atcaccacat gccgcgcggt gtatccctgg
      601 cgactaatga cagccaacaa gtcttcactg ctcgccgccc cccacttatc actctctttg
      661 gtcttcgcca gaatgctgaa aagggagact tcccgatcct caagcttttc ccaggtgtgt
      721 ttggtgtcgc accaggcaca gccaaccggg catccctgta aacgaataaa aatggcgggg
      781 acgccggtaa agtaaccctc accttgcagg gtctggaaca tctcgttaat cgggtactgc
      841 atagcattct ctgtgaagtg gataattgtt aattattgca gatcctgcca caacaatcat
      901 gtcttattaa cattctgtta caggcagatt taaagaaaaa tgcgaaacat atcgttaa
//
LOCUS       DAEEKQ010000423          948 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.432, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000423 DAEEKQ010000000
VERSION     DAEEKQ010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.432"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(108..683)
                     /locus_tag="KLK57_004553"
     CDS             complement(108..683)
                     /locus_tag="KLK57_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0894706.1"
                     /translation="MSRTFAYCRVSVETSTTENQIQAIKKAGYDIPKSRVIEEVVSGS
                     VSAMDRDGFANLVNHKLEEGDTLVVLKLDRLGRDNIDLQNTVTLLNDKGVKLICLDLP
                     VKDLSSSEGKLVLQLFSTFAEFERNRIRERTKQGLERARVQGKVLGRPIASDTTRAVL
                     EYKERGLSQSQVAKVLNLSVRTVSRHWTKTL"
BASE COUNT          258 a          210 c          200 g          280 t
ORIGIN      
        1 tactggccta tacaacgcta taaagacctc tacatagcgc cttgatagct atgtcacagg
       61 tacaaaaaag ccccctataa aggaggctgt gtagtctatt aagggtctta cagggtcttg
      121 gtccagtgtc tgctaacagt ccttacagag aggttaagaa ccttagctac ctgagactga
      181 gatagtcccc tttccttgta ctctaagacc gctctagttg tgtcactcgc tataggtctt
      241 ccaagtacct taccttgaac tctagcacgc tctagaccct gtttagtgcg ctctctgata
      301 cggtttcttt caaactctgc aaaggtacta aatagctgta gaaccaactt accctctgag
      361 cttgaaaggt ctttaacggg taaatctaaa cagatgagct tcacaccctt atcattaaga
      421 agggtcactg tattctgtag gtcgatgtta tcccttccca gacggtcaag tttaagaaca
      481 acaagggtgt caccttcttc tagcttatgg ttgaccagat tcgcaaatcc gtccctatcc
      541 atagctgaca ctgaaccaga gacaacctcc tctataactc gtgacttagg aatatcatag
      601 ccagccttct taatggcttg aatctggttc tctgtggtgg aagtctctac tgatacacgg
      661 cagtaagcaa aggttcgtga catagatatc cctctgggta gtcattaaac ggttagtttg
      721 tcttgtctat agacattaaa gcaaatggta gatttgttgt ctatagctat agtgactctt
      781 ctatgtagac attaaacgga cgtttcttgt cgtgggtcac atagatactt ttagggcttc
      841 tgtcaagtga tattcgtaga ttttgcaagg gggaggtgta aggctgaaac agggctacta
      901 agaacacata catctaaggc tggaaagtac ctgagcagat attcttca
//
LOCUS       DAEEKQ010000424          940 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.433, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000424 DAEEKQ010000000
VERSION     DAEEKQ010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..940
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.433"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>940
                     /locus_tag="KLK57_004554"
     CDS             <1..>940
                     /locus_tag="KLK57_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element IS4 family transposase"
                     /protein_id="HBE0894707.1"
                     /translation="TLLAIDGVFWRTPDTPENDAAFPRQTHAGNPALYPQVKMVCQME
                     LTSHLLTAAAFGTMKNSENELAEQLIEQTGDNTLTLMDKGYYSLGLLNAWSLAGEHRH
                     WMIPLRKGAQYEELRKLGKGDHLVKLKTSPQARKKWPGLGNEVTARLLTVTRKGKVCH
                     LLTSMTDAMRFPGGEMADLYSHRWEIELGYREIKQTMQLSRLTLRSKKPELVEQELWG
                     VLLAYNLVRYQMIKMAEHLKGYWPNQLSFSESCGMVMRMLMTLQGASPGRIPELMRDL
                     ASMGQLVKLPTRRERAFPRVVKERPWKYPTAPKKSQS"
BASE COUNT          272 a          222 c          277 g          169 t
ORIGIN      
        1 accctgctgg ccatcgatgg tgtgttctgg cgcacaccgg atacaccaga gaacgatgca
       61 gccttccccc gccagacaca tgccgggaac ccggcgctct acccgcaggt caaaatggtc
      121 tgccagatgg aactgaccag ccatctgctg acggctgcag ccttcggcac gatgaagaac
      181 agcgaaaatg agcttgctga gcaacttata gaacaaaccg gcgataacac tctgacgtta
      241 atggataaag gttattactc actgggactg ttaaatgcct ggagcctggc gggagaacac
      301 cgccactgga tgatacctct cagaaaggga gcgcaatatg aagagctcag aaaactgggt
      361 aaaggcgatc atctggtgaa gctgaaaacc agcccgcagg cacgaaaaaa gtggccggga
      421 ctgggaaatg aagtgacagc ccgcctgctg accgtgacgc gcaaaggaaa agtctgccat
      481 ctgctgacgt cgatgacgga cgccatgcgc ttccccggag gagaaatggc ggatctgtac
      541 agtcatcgct gggaaatcga actgggatac agggagataa aacagacgat gcaactgagc
      601 aggctgacgc tgagaagtaa aaagccggag cttgtggagc aagagctgtg gggtgtctta
      661 ctggcttata atctggtgag atatcagatg attaaaatgg cggaacatct gaaaggttac
      721 tggccgaatc aactgagttt ctcagaatca tgcggaatgg tgatgagaat gctgatgaca
      781 ttgcagggcg cttcaccggg acgtataccg gagctgatgc gcgatcttgc aagtatggga
      841 caacttgtga aattaccgac gagaagggaa agggccttcc cgagagtggt aaaggagagg
      901 ccctggaaat accccacagc cccgaaaaag agccagtcag
//
LOCUS       DAEEKQ010000425          933 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.435, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000425 DAEEKQ010000000
VERSION     DAEEKQ010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 933)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..933
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.435"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            13..837
                     /gene="gadX"
                     /locus_tag="KLK57_004555"
     CDS             13..837
                     /gene="gadX"
                     /locus_tag="KLK57_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HBE0894708.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
BASE COUNT          273 a          178 c          207 g          275 t
ORIGIN      
        1 attaagtcaa ctatgcaatc attacatggg aattgtctaa ttgcgtacgc aagacataaa
       61 tatattctca ccatggttaa tggtgaatat cgctatttta atggcggtga cctggttttt
      121 gcggatgcaa gccaaattcg agtagataag tgtgttgaaa attttgtatt agtatcaagg
      181 gatacgcttt cattatttct gccgatgctc aaggaggagg cattaaatct tcatgcacat
      241 aaaaaaattt cttcattact cgttcatcac tgtagcagag atattcctgt ttttcaggaa
      301 gttgcgcaac tatcgcagaa taagaatctt cgctatgcag aaatgctacg taaaagagca
      361 ttaatctttg cgttgttatc tgtttttctt gaggatgagc actttatacc gctgcttttg
      421 aacgttttgc aaccgaacat gcgaacacga gtttgtacgg ttatcaataa taatatcgcc
      481 catgagtgga cactagcccg aatcgccagc gagctgttga tgagtccaag cctgttaaag
      541 aaaaaattgc gcgaagaaga gacatcatat tcacagttgc ttactgagtg tagaatgcaa
      601 cgtgctttgc aacttattgt tatacatggc ttttcaatta agcgagtcgc agtgtcctgt
      661 ggatatcaca gcgtgtcgta tttcatttac gtctttcgaa attattatgg gatgacgccc
      721 acagagtatc aggagcgatc ggcgcaggga ttgccgaacc gtgactcggc ggcaagtatt
      781 gttgcgcaag gaaattttta cggcactgac cgttctgcgg aaggaataag attatagaat
      841 tttactcaga cataaaaaaa acccggcata ggggaccggg aagaggatag tctgccgtct
      901 ccagactaat aaaccgttat aacactccct gtt
//
LOCUS       DAEEKQ010000426          926 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.436, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000426 DAEEKQ010000000
VERSION     DAEEKQ010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..926
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.436"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            178..423
                     /locus_tag="KLK57_004556"
     CDS             178..423
                     /locus_tag="KLK57_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894709.1"
                     /translation="MTAKHIKKSQSHALDLTEHWLRVSIKIIDRNAGEGYAKAHPELI
                     SAFMTTAAANFATLTEREIAEAEQVTTINVKTGEQTA"
     gene            420..842
                     /locus_tag="KLK57_004557"
     CDS             420..842
                     /locus_tag="KLK57_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001758993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HBE0894710.1"
                     /translation="MTAQIAAYGRLVADPQLKTTSKGTQMTMASMAVPLPCSQADDGT
                     ATMWLSVLAFGRQAEALAKHRKGELLSVAGNMQVSQWTGQNGETRQGWQVIADSVISA
                     RTARPGGKKGQQGQATDALSRAKQQAGNDDPYGDNIPF"
BASE COUNT          276 a          208 c          273 g          169 t
ORIGIN      
        1 tcaccttttt gattgtttat tgagcttcaa gggtgaacag tggtgaacag ttggtgaata
       61 gtttttgtga aactgttcac cccttaacat tatgaattaa aagataaaat atcaaaaggt
      121 gaacaggtga agggttaaaa cgcaaaaatt ttaatttact gctgtgagat aaagcctatg
      181 acagcgaagc acataaaaaa atcacaatcg cacgcccttg atttgacgga acactggtta
      241 agggtgtcga taaaaatcat cgaccgcaac gccggggaag gatatgcgaa agcacatccc
      301 gaactgatta gcgcattcat gacaacggca gctgcaaact ttgccacgct gacagaacgg
      361 gagattgccg aagcggaaca ggtgacaacc atcaacgtta aaaccggaga gcagacagca
      421 tgacagcaca gatagcggct tacgggcggc tggtggctga cccgcagtta aagaccacca
      481 gcaagggtac acaaatgacg atggcgagta tggcggtccc acttccgtgc agccaggcag
      541 atgacggaac ggcgacgatg tggttatccg tcctggcgtt tggcagacag gccgaagcac
      601 tggcaaagca ccgcaagggt gaactcctga gcgtggcggg taacatgcag gtgagccagt
      661 ggaccggaca gaacggggag acgcggcagg gctggcaggt tatcgcagac agcgtaatca
      721 gtgcgagaac ggcgcgaccg ggcggcaaaa aaggccaaca ggggcaggct actgatgcac
      781 tgagcagagc aaaacaacag gcgggaaatg atgatccgta cggggataac ataccgtttt
      841 aagcaacgag taacagaagc cggagcaatc cggctttttt atgggtcctc ccggtgtagt
      901 gacctgccac ggggcgggag cgtcgc
//
LOCUS       DAEEKQ010000427          906 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.437, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000427 DAEEKQ010000000
VERSION     DAEEKQ010000427.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 906)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..906
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.437"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="KLK57_004558"
     CDS             complement(<1..752)
                     /gene="ipaH3"
                     /locus_tag="KLK57_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase IpaH3"
                     /protein_id="HBE0894711.1"
                     /translation="MSIMLPINNNFSLSQNSFYNTISGTYADYFSAWDKWEKQALPGE
                     NRNEAVSLLKECLINQFSELQLNRLNLSSLPDNLPPQITVLEITQNALISLPELPASL
                     EYLDACDNHLSTLPELPASLKHLDVDNNQLTMLPELPALLEYINADNNQLTMLPELPT
                     SLEVLSVRNNQLTFLPELPESLEALDVSTNLLESLPAVPVRNHHSEETEIFFRCRENR
                     ITYIPENILSLDPTCTIILEDNPLSSRIRESLS"
BASE COUNT          267 a          144 c          222 g          273 t
ORIGIN      
        1 gacagagact ccctgatccg tgaggacaga ggattgtctt cgaggatgat agtgcaggtc
       61 ggatcaaggc taagtatatt ttccggaatg tatgtgatgc gattctcgcg gcaccggaaa
      121 aatatctcgg tttcctctga gtgatgattt cttacaggta cggctggtag gctttccaga
      181 agattagtac ttacatcgag cgcttccagt gattcaggta actcagggag aaatgtcagc
      241 tggttatttc ttactgagag cacttccagc gatgtaggta attcaggaag catggttagc
      301 tgattgttat ctgcattaat atattccagc aatgcaggca attcaggaag catggttagt
      361 tggttgttat ctacatcaag atgtttcaga gatgcgggta attcaggaag tgttgacagg
      421 tgattgtcac aggcgtcaag gtattccagc gatgctggca attctggtaa tgatattagg
      481 gcattctgag taatttccag aacagtgatt tgaggtggta agttgtcagg tagcgaggac
      541 agatttaaac gattcagttg aagctcactg aactgattga tgagacattc tttaagtagg
      601 gagaccgctt cattccgatt ttcaccgggg agcgcttgtt tttcccattt atcccatgct
      661 gaaaagtaat cagcatatgt accggaaata gtgttataaa aagaattttg ggacaatgaa
      721 aagttattat ttatcggtaa catgatggac attcagttaa agccgatgga caaatggctt
      781 tgttaaaaga aataagtagg agtttttatg aagttcattc catgaaatga acttcagcac
      841 ttttttatta taaaaggaca aacaaaattg gctgtaaaaa aacgatactg tttcagttaa
      901 ctgtga
//
LOCUS       DAEEKQ010000428          880 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.438, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000428 DAEEKQ010000000
VERSION     DAEEKQ010000428.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 880)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 880)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..880
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.438"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>880
                     /gene="cadA"
                     /locus_tag="KLK57_004559"
     CDS             <1..>880
                     /gene="cadA"
                     /locus_tag="KLK57_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HBE0894712.1"
                     /translation="NFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVK
                     GDVNEETFNEAYMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEI
                     KRLRTESDGWFFDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPG
                     MEKDGTMSNFGIPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTD
                     FKRAFDLNLRVKNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVL
                     PTMVMTPYAAFQKELHG"
BASE COUNT          238 a          222 c          214 g          206 t
ORIGIN      
        1 ccaacttctc accgatttac gaaggtaaat gcggtatgag cggtggccgt gtagaaggga
       61 aagtgattta cgaaacccag tccactcaca aactgctggc ggcgttctct caggcttcca
      121 tgatccacgt taaaggtgac gtaaacgaag aaacctttaa cgaagcctac atgatgcaca
      181 ccaccacttc tcctcactac ggtatcgtgg cgtccactga aaccgctgcg gcgatgatga
      241 aaggcaatgc aggtaaacgt ttgatcaacg gttccattga acgtgcgatc aaattccgta
      301 aagagatcaa acgtctgaga acggaatctg atggctggtt ctttgatgta tggcagccgg
      361 atcatatcga tacgactgaa tgctggccgc tgcgttctga cagcacctgg cacggcttca
      421 aaaacatcga taacgagcac atgtatcttg acccgatcaa agtcaccctg ctgactccgg
      481 ggatggaaaa agacggcacc atgagcaact ttggtattcc ggccagcatc gtggcgaaat
      541 acctcgacga acatggcatc gttgttgaga aaaccggtcc gtataacctg ctgttcctgt
      601 tcagcatcgg tatcgataag accaaagcac tgagcctgct gcgtgctctg actgacttca
      661 aacgtgcgtt cgacctgaac ctgcgtgtga aaaacatgct gccgtctctg tatcgtgaag
      721 atcctgaatt ctatgaaaac atgcgtattc aggaactggc tcagaatatc cacaaactga
      781 ttgttcacca caatctgccg gatctgatgt atcgcgcatt tgaagtgctg ccgactatgg
      841 taatgactcc gtatgctgcg ttccagaaag agctgcacgg
//
LOCUS       DAEEKQ010000429          877 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.439, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000429 DAEEKQ010000000
VERSION     DAEEKQ010000429.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..877
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.439"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            49..849
                     /locus_tag="KLK57_004560"
                     /pseudo
     CDS             49..849
                     /locus_tag="KLK57_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020218947.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT          252 a          170 c          211 g          244 t
ORIGIN      
        1 gcacttattg atatattcat gaagattata atcacaaggg aatacataat gaaaagaaaa
       61 gttctggcaa tgctggtccc agcgttatta gttgctggcg cagcaaatgc ggctgaaatt
      121 tataataaaa atggcaataa actggatctg tacggaaaag ttgatgctcg tcatactttt
      181 tccgataacc ctggcgatga cggcgatgaa actatcatcg agttaggctt caaaggtgag
      241 acccaaatta cagatcagtt aactggttat ggtcaagcac tgaccaaaac aaaggctagc
      301 gacactgaag gttctgataa tacttatgtc aaacttgctt ttgcaggtct gaagttcggc
      361 gagatgggct cctttgacta tggtcgtaac tatggtgtga tttacgacgt tgaagcctgg
      421 acagatatgc ttcctgtatt cggcggcgac tcctatacct ggactgataa cttcatggca
      481 ggccgtgcaa atggggtagc tacttaccgc aatagtgatt tcttcggtct ggtggaaggt
      541 ctgaactttg cattgcagta tcaaggtaac aacgaaggca gcaatgcagg agaagatcaa
      601 gaaggtacta aaaatggtca cgaagacgtt cgcttccaga acggtgatgg ttttggtctt
      661 tccacttcat atgactttga cttcggtttg agcttaggtg ccgcttactc aaactctgac
      721 cgtaccaatt ctcaagtagc tcttggcggg tatcactaca atgaatacag caaatttgct
      781 ggtggtgata cagctgaagc atggacattt ggtgcaaaat acgacgccaa caatgtttat
      841 cttgcaatgg gtgatgctgc caacttactg atttagt
//
LOCUS       DAEEKQ010000430          871 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.440, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000430 DAEEKQ010000000
VERSION     DAEEKQ010000430.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.440"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..93
                     /locus_tag="KLK57_004561"
     CDS             <1..93
                     /locus_tag="KLK57_004561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HBE0894713.1"
                     /translation="HPHFVLVYEVDSQWGKVYVLRVLHTAQKWP"
     gene            complement(289..630)
                     /locus_tag="KLK57_004562"
     CDS             complement(289..630)
                     /locus_tag="KLK57_004562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894714.1"
                     /translation="MIFTKFQSLTHKIDTMIIHDIKREMPLKYGLYRVAKWFAWLAHT
                     GIFCTFIIYIGFSIITQHAGQELPETFKHGFALTFCSFATAALVSQWIGGGLHSKLEE
                     RIRMKWQNHAH"
BASE COUNT          216 a          204 c          195 g          256 t
ORIGIN      
        1 cacccccatt ttgttctggt ttatgaggtc gacagccagt ggggaaaagt gtatgtcctg
       61 cgcgtgttgc ataccgcaca gaagtggcca taaaattcca gtcaggaata atacttttca
      121 tttttagttt actgctcccc cagtaagagc gatatattcc gaacagtctt gtctgggagg
      181 tatggtgcag tcagcgaaga accgccgaga atcctcgccc aatgcgggcg gggagcagtc
      241 accggagtct tcgccacccg ccctctgtgc actgaccggg tgatcctgtc agtgagcgtg
      301 gttctgccac ttcatccgaa ttctttcttc cagtttgcta tgcaatccgc ctccgatcca
      361 ctggctgacc agagctgctg ttgcaaatga acagaaagtc aaggcgaatc catgtttaaa
      421 cgtctccggt agttcctggc cggcgtgctg agtgatgatg gaaaacccta tatatataat
      481 aaaggtacaa aaaattcctg tgtgggcgag ccaggcaaac catttggcca ccctgtataa
      541 cccgtatttc aatggcatct cccgtttaat atcatgaata atcattgtat ctatcttgtg
      601 ggtcagagat tgaaattttg taaatatcat aagtccttag tccatctaaa gtgagattta
      661 ccgtatctga atatatcaaa atgctaaggc agaatccccg gaaatttgtg tattacttct
      721 gtagcagaca cggcaaaatc gttgcattac aggtgcatct gctggggcgt ctgtttatcg
      781 atgcatcaga tttaatgtct gacagttact tttctgtatc acccacactc tctgcttttt
      841 cattgctact gtcatattta tgtctgttgc g
//
LOCUS       DAEEKQ010000431          844 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.441, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000431 DAEEKQ010000000
VERSION     DAEEKQ010000431.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.441"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(279..386)
                     /locus_tag="KLK57_004563"
     CDS             complement(279..386)
                     /locus_tag="KLK57_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HBE0894715.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(762..>844)
                     /gene="ldrD"
                     /locus_tag="KLK57_004564"
     CDS             complement(762..>844)
                     /gene="ldrD"
                     /locus_tag="KLK57_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxic polypeptide
                     LdrD"
                     /protein_id="HBE0894716.1"
                     /translation="FWHDLAAPVIAGILASMIVNWLNKRK"
BASE COUNT          178 a          259 c          196 g          211 t
ORIGIN      
        1 acgtgcgggg gttttctctc tccagcaacc aatgccacca gggataaagc ccccgcaaca
       61 ttgcgcctca ccggaaccct tccggcttga cgctgattct acgactgttt ttctgttccg
      121 gaatccgtca cgtcttcact ctgtgctgcg cattccgcag aatattatcc gcttgcaacc
      181 gttgcatttt tcttacgaaa aaggccgcag agtttcaccc ctgcggcctg atccgggcgc
      241 aaattgccat tacggcagcc tgacgcccgc atggcacgtt acttccgctt gttcagccag
      301 ttcacgatca tactggcaag aataccagca atgaccggag ccgctaaatc atgccagaag
      361 gccatgccca gttctgcgag cgtcatatag ccgcctgtat tgtaatgaca acgtttcgcg
      421 gctattcttg tattgtttgg atgcaagaat ggcccccgtg gtgttgtcag gtgcatacct
      481 gcaacgtgcg gggggtttct ctctccagca accaatgcca ccagggatta agcccccgca
      541 acattgcgcc tcaccggaat ccttccggct tgacgctgat tctacgactg tttttctgtt
      601 ccggaatccg tctcatcttc actctgtgct gcgcattccg cagaatatta tccgcttgca
      661 accgttgcat ttttcttacg aaaaaggccg cagagtttca ctcctgcggc ctggtccggg
      721 cgcaaattgc cattacggca gcctgacgcc cacatgacac gctacttccg cttgttcagc
      781 cagttcacga tcatactggc aagaatgcca gcaatgaccg gagccgctaa atcatgccag
      841 aagg
//
LOCUS       DAEEKQ010000432          842 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.442, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000432 DAEEKQ010000000
VERSION     DAEEKQ010000432.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 842)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..842
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.442"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            54..130
                     /locus_tag="KLK57_004565"
     tRNA            54..130
                     /locus_tag="KLK57_004565"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:88..90,aa:Ile,seq:gat)
     gene            173..248
                     /locus_tag="KLK57_004566"
     tRNA            173..248
                     /locus_tag="KLK57_004566"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:206..208,aa:Ala,seq:tgc)
     gene            432..>842
                     /locus_tag="KLK57_004567"
     rRNA            432..>842
                     /locus_tag="KLK57_004567"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          238 a          177 c          232 g          195 t
ORIGIN      
        1 ctttgcagtg ctcacacaga ttgtctgatg aaaatgagca gtaaaacctc tacaggcttg
       61 tagctcaggt ggttagagcg cacccctgat aagggtgagg tcggtggttc aagtccactc
      121 aggcctacca aatttgcatc gcaaatttga agaggtttta actacatgtt atggggctat
      181 agctcagctg ggagagcgcc tgctttgcac gcaggaggtc tgcggttcga tcccgcatag
      241 ctccaccatc tctgtagtga ttaaataaaa aatacttcag agtgtacctg caaaggttca
      301 ctgcgaagtt ttgctcttta aaaatctgga tcaagctgaa aattgaaaca ctgaacaatg
      361 aaagttgttc gtgagtctct caaattttcg caacacgatg atgaatcgca agaaacatct
      421 tcgggttgtg aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga
      481 tgaaggacgt gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc
      541 gatttccgaa tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg
      601 ttaatgaggc gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc
      661 gagattcccc cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg
      721 tgttagtgga agcgtctgga aaggcgcgcg atacagggtg acagccccgt acacaaaaat
      781 gcacatattg tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg
      841 gg
//
LOCUS       DAEEKQ010000433          832 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.443, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000433 DAEEKQ010000000
VERSION     DAEEKQ010000433.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 832)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 832)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..832
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.443"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..832
                     /locus_tag="KLK57_004568"
                     /pseudo
     CDS             1..832
                     /locus_tag="KLK57_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312403.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          322 a          163 c          122 g          225 t
ORIGIN      
        1 gtataataaa tacatatcac ggcgatattt ataactgcac aacaaatacc gataatgcta
       61 aaacgccaga cacaccgaag tggccttgcg ataattggga agaacaacaa cccattaatt
      121 ccacattctc tggagaagga tatatctctg atcaatatga tttagtgcaa caccaacttc
      181 aacaaattaa tgcgtgccac acgaacacta catatacaaa tgcagactac tcaaaagttg
      241 tggctcaact tgtaagtctt attactaaca ttgaaacgat aagttcgaca caacttacgc
      301 aacagacaca atcaatactt aatcagatta ataacattag atatgaaaaa aacaaaagtg
      361 ctgaatgtcg aatcattgtt gtcgctaatc ctaaacccga caaggcaatc ataactaaaa
      421 ttagcgtaga agagggaata cccataacat tcagtgtcca aaccatgttt tcagatacta
      481 attttattgc tgaacaacga gctgacttac caactaatat aaaagatatt caaagcctgt
      541 atcaaaaaat gacgaagtta tatattgagc acagtgaaaa taaaaacagg atgaaagtct
      601 ttgccggagc caactttatt gatttcaata tgacaggaca gaacctgtcc gggttcgttc
      661 taaccttgtc aaggttttat ttcgaagatc tacttaatat aaatttcact gacgcaaatt
      721 taggtgacac tattttttac ataaagaaca ccctaccccc aaattatata aagatggaca
      781 atatcttgac aaacaaatcg aaggtttatt ttcaacatta ttaaccatta at
//
LOCUS       DAEEKQ010000434          813 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.444, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000434 DAEEKQ010000000
VERSION     DAEEKQ010000434.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 813)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 813)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..813
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.444"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>813
                     /locus_tag="KLK57_004569"
     CDS             <1..>813
                     /locus_tag="KLK57_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HBE0894717.1"
                     /translation="SPLPASLTQLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLP
                     ISLKELHVSNNQLCSLPVLPELLETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPP
                     LPHNIHSIWAIDNMLTDIPYLPENLRNGYFDINQISHIPESILNLRNECSIDISDNPL
                     SSHALQSLQRLTSSPDYHGPQIYFSMSDGQQNTLHRPLADAVTAWFPENKQSDVSQIW
                     HAFEHEEHANTFSAFLDRLSDTVSARNTSGFREQVAAWLEKLSASAELRQQSF"
BASE COUNT          225 a          221 c          141 g          226 t
ORIGIN      
        1 tccccattgc ctgcgtccct tacacagctt aatgtcagct ataacagact tattgaactg
       61 cctgctttgc ctcaaggact taaattattg aatgcgtccc acaatcaact aatcacacta
      121 cccacactcc ccatatcttt gaaggagctt catgtctcaa ataatcaatt atgttctctt
      181 cctgttttac cagaactact ggaaacatta gatgtatcat gtaatgggct ggcagtttta
      241 ccacctttac cattttcttt acaagagatt agcgcaatag ggaatcttct tagtgaactc
      301 ccccctctac ctcacaacat tcactccata tgggcaatcg acaatatgtt aaccgatatt
      361 ccatacctgc cggaaaattt aaggaacggt tattttgaca taaatcagat aagtcatatc
      421 ccggaaagca ttcttaatct gaggaatgaa tgttcaatag atattagtga taacccattg
      481 tcatcccatg ctctgcaatc cctgcaaaga ttaacctctt cgccggacta ccacggcccg
      541 cagatttact tctccatgag tgacggacaa cagaatacac tccatcgccc cctggctgat
      601 gccgtgacag catggttccc ggaaaacaaa caatctgatg tatcacagat atggcatgct
      661 tttgaacatg aagagcatgc caacaccttt tccgcgttcc ttgaccgcct ttccgatacc
      721 gtctctgcac gcaatacctc cggattccgt gaacaggtcg ctgcatggct ggaaaaactc
      781 agtgcctctg cggagcttcg acagcagtct ttc
//
LOCUS       DAEEKQ010000435          803 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.445, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000435 DAEEKQ010000000
VERSION     DAEEKQ010000435.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.445"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(68..619)
                     /locus_tag="KLK57_004570"
     CDS             complement(68..619)
                     /locus_tag="KLK57_004570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HBE0894718.1"
                     /translation="MAILGYCRVSTDDQSITNQQMQIEEAGYNIAKWFADEAVSGSVK
                     ASLRNGFSSLLAYAREGDTVVVVAVDRLGRDTIDVLSTVKALQAKGVTVISLREGFDL
                     SSAMGEAMLGIMSTLAQLERSLIAERRKAGIERAKAEGVHMGRPVKASSEAVQMLISQ
                     GKTRLQIQEELGISRATYYRLAK"
BASE COUNT          225 a          202 c          137 g          239 t
ORIGIN      
        1 accagctatg tgaccccctt ggcaaaaaag ccaaatatca gctacatcca tgtaactatt
       61 cagtaactta tttagcaagt ctgtaataag ttgctctaga gattcctagc tcttcctgaa
      121 tctgcaacct cgttttacct tgtgaaataa gcatctgtac agcctctgaa gaagctttta
      181 caggtcttcc catatgtacc ccttcagctt tagcacgttc aataccagcc tttctacgct
      241 ctgctatcaa gcttctttca agctgtgcca acgttgacat aatgcctaac attgcttcac
      301 ccatagcgct tgataagtca aatccttctc taaggctgat aactgtcaca cctttagcct
      361 gtagcgcttt aaccgttgat aacacatcaa ttgtgtcacg tccaagacgg tctacagcta
      421 ccacaactac ggtgtcacct tctcgtgcat aagctaacaa actgctgaaa ccgtttctta
      481 aagaagcttt gacagaacca ctgacagctt catcagcgaa ccatttagca atgttgtacc
      541 ctgcctcttc aatttgcatt tgctggttag tgatggattg gtcatctgta ctcaccctgc
      601 aatatccaag tatcgccata atgttcccct tagaagtctc aaaagttagt ctcataagct
      661 tagctgtctc ataatccaaa aacaaccttt tgagactact tgagggtgca ttctaccgtt
      721 acgttgaaag tgtctcaaaa ggacttcctt atgagactgg ttaatagagc gctatgaaga
      781 ttcaggaggg taactataac cat
//
LOCUS       DAEEKQ010000436          797 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.446, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000436 DAEEKQ010000000
VERSION     DAEEKQ010000436.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.446"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..743)
                     /locus_tag="KLK57_004571"
     CDS             complement(<1..743)
                     /locus_tag="KLK57_004571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HBE0894719.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQLAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKLAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVCISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCV"
BASE COUNT          268 a          138 c          160 g          231 t
ORIGIN      
        1 acacataaac aggtaaagac cataaatatg gtgaaagtaa aaaagaagga aaatacagca
       61 atggaaaaaa ttttatcgga gaacaggaat aaatctaaaa gatagattgc tgaaatacag
      121 actccacttt tacatgcgcg acaaaaatta ctgccttgat gaaaaaacca gaaagggaag
      181 aacatcactg caaataccca aaaaatagca aagtaataat gatcactgga agacgctgat
      241 tcactttctg aaggattagt cggatattgg tgttgtgaag tgagttggtt attcaaagcg
      301 tttatagcta gctctaatcc ctgagctaat ttttgttgtt taaatgcggg tatcatgttg
      361 ctacggatga tatctccagc cagagcatcg gttacctttt cttccagccc ataacctacc
      421 tggatgcgga cagtacgatc tgaccaggca acaatgatca gtatcccatc attacgtttg
      481 gcatctccta aacgccaatt gtcaaaaact cttgtagcat attgttcaat tgtctcatct
      541 ttggttgtgg gaacaattaa tacagcaagt tgcacttggg tattattgaa aatagattct
      601 aacttgtgcg tcagttcttt cttttcttgt gagtttaagc tactcgtaag atccgtaact
      661 tgttgctgta attgtgggac aatagttggt tgggctgcaa caaaggaaca cacgaaagtg
      721 aacattagta tgaaatagcg cattataaat ttctcattaa atctggataa ataaattgac
      781 ggcggcatcg gtaattt
//
LOCUS       DAEEKQ010000437          797 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.447, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000437 DAEEKQ010000000
VERSION     DAEEKQ010000437.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.447"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..637
                     /locus_tag="KLK57_004572"
     CDS             <1..637
                     /locus_tag="KLK57_004572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894720.1"
                     /translation="LVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMDYP
                     DHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFINSVRTDCKC
                     ILLEHHMPLLRLFPKGKECMHVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYPVF
                     PESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
BASE COUNT          231 a          159 c          171 g          236 t
ORIGIN      
        1 tttagtcgat gacgacaaac tcattcatct catggctgcc gtacgagact gtgagtggtc
       61 agacgataac gcactcacca taaatgtgca gtttaatgat ttcccaggat tttatgactg
      121 gatggattac cctgatcacc cggttaagtt tgtttttcgc atacttgaaa atcaaaaagg
      181 gacagtttgg gtatatgacc aggatgatgc gtttcttgat attaaagcaa atgtgcaggc
      241 tggacgcttt accgggctta agaaattagt gcagtttatc aattcagtgc gaacagattg
      301 caaatgtatt cttcttgaac accatatgcc tttgctgaga cttttcccta aagggaaaga
      361 gtgtatgcat gtggaaaaat ggcttcgcga aatgtcgtct attccggaga cagacgcgcc
      421 cattaagcag gcgcttgcgc atggattatt attacactta aaaaatatct accctgtttt
      481 tcctgaatca ctggtgatgt tattgctctc tgttctggac gtaaaaacat acagagatga
      541 tgcacgatta aatgaatgga tatctaatag agtgcaggaa ttaggtgaca gatattatcc
      601 tgtaaataag cacgttaaaa tccgctacac tctttagttc agaaagggtt ccacctgcaa
      661 gttgttattc caccagagag tgaatcgcaa agcaggtaat catttcatct gtatatccgt
      721 atgccaaatg gcattccacc gccttttcgc cgatattttg ttgtgatcgg cgacacttcg
      781 cttaaaaaag caccagc
//
LOCUS       DAEEKQ010000438          792 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.448, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000438 DAEEKQ010000000
VERSION     DAEEKQ010000438.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..792
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.448"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(64..726)
                     /locus_tag="KLK57_004573"
     CDS             complement(64..726)
                     /locus_tag="KLK57_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HBE0894721.1"
                     /translation="MSIEHILIGKHHGGSDYWRTPLLLFKNLHREFGFTLDGAATEHD
                     TLLPRFTNDISRQSWVSEKVFCNLPYSDIPSFLLKASEADLVAFLIPHRANTSYWLRH
                     IYSNNHCHEIRILHRAVKYLPPAGHNGLTIRSPFPSAVVVFKKEPRKHEITQTVCCGD
                     TLLPLTIINRGGLRGRPTIYPPETLDSFIKLYRQGKPIKCIADALRMPLSTSYRIAQR
                     LS"
BASE COUNT          227 a          158 c          170 g          237 t
ORIGIN      
        1 tcacagggat acttcttaat atctgaatca cctatcttgt aaatgaccat atttatgaga
       61 attttagctc aacctttgag caatcctgta tgacgtgctc agaggcattc tgagagcatc
      121 tgcaatgcac ttaatgggtt ttccttgcct gtatagtttt atgaagctgt cgagcgtctc
      181 aggtgggtaa atggtgggtc ttcctctcaa gcctcctctg ttaatgatgg ttaacggtaa
      241 tagcgtgtca ccacagcaaa ccgtctgtgt aatttcgtgc ttacgtggtt cttttttaaa
      301 aacaacgact gcactaggaa acggtgaacg aatagtaagc ccgttatggc ctgctggtgg
      361 aaggtattta actgcacgat gaagaatacg gatttcatga cagtggttat tgctgtaaat
      421 gtgcctaagc cagtagctgg tgttggctct atgcggtatc aggaaagcta ctaaatccgc
      481 ttcagacgct tttaagagga acgacggaat atcgctgtac ggcaggttac agaagacctt
      541 ttcactaacc catgattgac ggcttatatc attggtgaat cgtggtaaca gcgtgtcatg
      601 ctcagttgca gcaccatcta gcgtaaaacc aaattcacgg tgtaagttct taaataacaa
      661 caatggagtt ctccagtaat cagaaccccc atgatgtttc ccgattaaaa tatgctcaat
      721 tgacataaga aataaaaaat tttattgcaa tcactcttga caaggtttac tctcttttaa
      781 gataagagat ac
//
LOCUS       DAEEKQ010000439          790 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.449, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000439 DAEEKQ010000000
VERSION     DAEEKQ010000439.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.449"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            52..790
                     /locus_tag="KLK57_004574"
                     /pseudo
     CDS             52..790
                     /locus_tag="KLK57_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          228 a          194 c          208 g          160 t
ORIGIN      
        1 gccacctgaa cagaggtgat atgctcacct cagaacaaca caggtgctcc aatgaaaaaa
       61 agaaatttta gcgcagagtt taaacgcgaa tccgctcaac tggttgttga ccagaaatac
      121 acggtggcag atgccgccaa agctatggat gttggccttt ccacaatgac aagatgggtc
      181 aaacaactgc gtgatgagcg tcagggcaaa acaccaaaag cctctccgat aacaccagaa
      241 caaatcgaaa tacgtaagct gaggaaaaag ctacaacgca ttgaaatgga gaatgaaata
      301 ttaaaaaagg ctaccgcgct cttgatgtca gactccctga acagttctcg ataatcggga
      361 aactcagagc gcattatcct gtggtcacac tctgccatgt gttcggggtt catcgcagca
      421 gctacagata ctggaaaaac cgtcctgaaa aaccagacgg cagacgggct gtattacgca
      481 gtcaggtact tgagctacat ggcatcagcc acggttcggc cggagcaaga agcatcgcca
      541 caatggcaac ccggagaggc taccagatgg gacgctggct tgctggcagg ctcatgaaag
      601 agctggggct ggtcagctgt cagcagccga ctcaccggta taaacgtggt ggtcatgaac
      661 atgttgctat ccctaactac cttgaaaggc agttcgccgt gaccgagcca aatcaggtgt
      721 ggtgcggtga tgtgacctat atctggacgg gtaagcgctg ggcgtacctc gccgttgttc
      781 tcgacctgtt
//
LOCUS       DAEEKQ010000440          776 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.451, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000440 DAEEKQ010000000
VERSION     DAEEKQ010000440.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..776
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.451"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..344
                     /locus_tag="KLK57_004575"
     CDS             <1..344
                     /locus_tag="KLK57_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HBE0894722.1"
                     /translation="ADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVAHGELGGDFSLV
                     PDSYVTMGPKSIMAAKNLLIIVSGAGKAQALKNVLQGPVTEDVPASVLQLHPSLIVIA
                     DKAAAAELALG"
     gene            complement(406..>776)
                     /locus_tag="KLK57_004576"
     CDS             complement(406..>776)
                     /locus_tag="KLK57_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HBE0894723.1"
                     /translation="QDDASIEGVEFEYDEEDEFAGIKNTYPDEMLKELVERTPGYHGW
                     QQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGIRNSDLAKCLQKGGDC
                     QGYLFRCLHCGKLRLWGDFS"
BASE COUNT          210 a          190 c          176 g          200 t
ORIGIN      
        1 gtgcagatgg tcatttttgt ggcaatttgc cgaatacgac ccatttccat gagcaaaccg
       61 tggagttccc gatccaggga gagatggttg atattgtggc gcatggtgag ttgggcggcg
      121 acttttcgtt agtgcctgac agctacgtga cgatggggcc gaaaagcatc atggcggcga
      181 aaaacttgct gataattgtc agtggcgcag gtaaagcgca ggcactgaaa aatgtgctgc
      241 aaggtccggt taccgaagat gttccagctt ccgtgttgca gctgcatcct tcgctgatag
      301 taatcgctga taaagccgct gccgcagaat tagcgctcgg ttaagctgaa gaaataaggc
      361 cggatgtatg aattacgctc cggcctgttc tcattattta aataactacg aaaaatcacc
      421 ccacagtctc agcttgccgc agtggagaca gcggaagaga taaccctgac aatcaccacc
      481 cttttgcagg catttagcta gatcagaatt tctaataccg aaattctcac aatcttcttc
      541 aaggttggca aattcatcga ggcgatcttt tatatcattc cagcccacat agccgataaa
      601 agcacagaaa tcgccacaat gcgcaagcca gaattcctgt tgccatccat gataacctgg
      661 cgtgcgttca accaactctt tcagcatttc atcaggatat gtgttcttaa taccggcaaa
      721 ttcgtcctct tcatcatact caaattcaac accttctatg ctggcatcat cctgaa
//
LOCUS       DAEEKQ010000441          775 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.452, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000441 DAEEKQ010000000
VERSION     DAEEKQ010000441.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..775
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.452"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(55..351)
                     /locus_tag="KLK57_004577"
     CDS             complement(55..351)
                     /locus_tag="KLK57_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HBE0894724.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
BASE COUNT          230 a          165 c          155 g          225 t
ORIGIN      
        1 ggccaaacaa gaacacggtt gcaaaaaccg tgcccttaaa tattgaatct ctattcagaa
       61 cactttctta aatggtttca ctgaaacgtg ttcatagaca cctgccgcta cgtacgggtc
      121 agcatcggcc caggcctgag ctgcttccag cgattcaaat tcagcaataa cggttgagcc
      181 agtaaatccc gcagcccctg gatcgttact gtctaccgct ggcattggac cagctgtcaa
      241 caaacgacct tcatcatgca gtaactgtaa acgtgctaaa tgtgccggac gaacggaaag
      301 gcgtttttcg aggctatcag ctttgtcttg agcgtaaata acatacaaca cgggcacaac
      361 tccttgttcg ggaaagttgt aaagtacgtt atttgaaagg gcgaagatct gcaacggaaa
      421 gatgatgtct ttgttaagac catgcataaa gtaaggtcaa ttacgccaaa aatgacattt
      481 tcactgatcc tgatcgtctt gccttattga atatgattgc tatttgcatt taaaatcgag
      541 acttggtttt tctactgaaa tgattatgac ttcaatgacc cttgaatgaa gtctattcgg
      601 ttttctagga gacctgttaa aaacaggaca atatcaggtg agtccctatg caatttaata
      661 gtagatttcc aagtatctat gcaattctac acagattcta tacagtctcg gtatagtccc
      721 tttttaccct aacttattga aatatataaa gaatctgtga agtctctttt caggg
//
LOCUS       DAEEKQ010000442          771 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.453, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000442 DAEEKQ010000000
VERSION     DAEEKQ010000442.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.453"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            185..751
                     /gene="slp"
                     /locus_tag="KLK57_004578"
     CDS             185..751
                     /gene="slp"
                     /locus_tag="KLK57_004578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HBE0894725.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          235 a          161 c          172 g          203 t
ORIGIN      
        1 ggattatatt atggggcgat ggatactcat gccacgcccc aaaatcaatg gaattgaact
       61 aaacaaaagg gatataaaca tcagacaggt ttacgttact atcaggcata tcgcctcaga
      121 atcagatgaa aactataaag aaatatctat tatggtttta atatttgttg ataaggatag
      181 taacatgaac atgacaaaag gtgcactcat cctcagcctt tcatttttgc ttgccgcatg
      241 tagttcaatt ccgcaaaata tcaaaggcaa taaccaacct gatattcaaa aaagttttgt
      301 tgctgttcat aaccagccgg ggttatatgt tggtcaacaa gcgcgctttg gtgggaaggt
      361 tatcaacgtt atcaatggca aaacggatac gttgttagaa atcgctgtat taccgttgga
      421 tagctatgcg aaacctgata ttgaagccaa ctatcagggc cgactgctcg ccagacaaag
      481 cggcttcctt gacccagtga actatcgtaa tcactttgtt accatcctcg gtaccattca
      541 gggtgaacaa cctggcttta tcaataaagt cccgtataac ttcctggaag tgaatatgca
      601 gggcatccag gtgtggcatt tgagagaagt ggttaatacc acctataacc tgtgggatta
      661 cggctatggt gcattctggc cggaaccggg ctggggtgcg ccttactaca ccaatgcggt
      721 gagtcaggta acacctgagc tggtcaaata acaccaccga aagatgcaga t
//
LOCUS       DAEEKQ010000443          765 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.454, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000443 DAEEKQ010000000
VERSION     DAEEKQ010000443.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 765)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..765
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.454"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(1..374)
                     /locus_tag="KLK57_004579"
                     /pseudo
     CDS             complement(1..374)
                     /locus_tag="KLK57_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
BASE COUNT          191 a          209 c          172 g          193 t
ORIGIN      
        1 cgcgctgagg cgctcaaatc gagataatca gccagttcat cgagggtata ggcttcctct
       61 cgccactcca tcacctgcca ttccaggtgg aacggatttt tacaaatcag tccgctgtag
      121 ttcacatcag cacaaagttt ctcacacagc gaacgttcaa tcgcagcggc gtatttcagc
      181 gccttaaccg agctgtccgg cgcggttctc acggcgatat tgagcgcgta gagcaggtga
      241 gcatgaccgt tcacaggatt ttttacggtg atgttcggcg cgggagcgtt ccggtcgctc
      301 cagtcaatcg ctgccccctc gcgatcaacg tcaaaaacca gccagaactg cgcatgaggc
      361 tggttctgcc ggatctggac atgatttcag gggcgtttgt ttttcagtcg cttacactac
      421 acccaacggc aaatcctgtc gaaccttccc gaaaacccga tccatttacc ataactcccg
      481 ttgtacgtgc cctctcagac gctctctgac gcaaaaaact caaaaagaca agaaaaaaca
      541 gggtaaacat aaaaaatgcg tcagaatcgt ttttagcgcg ttgcataact atgcacgaaa
      601 gcgacccgcg atcggttttg tgtgttctga ataccgaacg ggtcaaaatg acacgttttc
      661 tctccctcaa tggagcgttc tatacagaga tttttgttcc cctatccatt attctgatta
      721 ccgggtttcc aagctgaaaa caggactttt ctgcgaccag ttttt
//
LOCUS       DAEEKQ010000444          752 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.455, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000444 DAEEKQ010000000
VERSION     DAEEKQ010000444.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..752
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.455"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..431)
                     /locus_tag="KLK57_004580"
     CDS             complement(<1..431)
                     /locus_tag="KLK57_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HBE0894726.1"
                     /translation="MVKSMKTNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIR"
     gene            488..>752
                     /locus_tag="KLK57_004581"
     CDS             488..>752
                     /locus_tag="KLK57_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HBE0894727.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDT"
BASE COUNT          223 a          150 c          172 g          207 t
ORIGIN      
        1 ttgcgtatta cacagctttt tgtagatata tttccaggca ggcagtgaag aaggtaaaac
       61 atggaaatgg aatcaaattg accatgtaac gccgcgggat aaggttcaaa aacatcatgg
      121 ctaattttat gtttaatttt tgattcccca gcccttgtag atgccgcgtt caggctagct
      181 tcgttcaaat ccattaaaga tatcagacta ctctcaggta cgtgagtaag gtaaaaccca
      241 gttccaacac caatatccag atggttgtta cctaaatgtt ccagaaagtg tggaagaagg
      301 tgttcctttg taggacatcc ccatgcaagc cgatttgata ctcccaaaac ccaccagtca
      361 taaagcttta gggtaagtgg tgtgtaaatt ttagccccat catctgtgtt tgttttcatt
      421 gatttcacca tgttatagtt ttatttgtga attaaatcaa ttatggcgat gaattacaag
      481 gggttaaatg ctgccgcagc atagcgatat tgaaatagcc tggtatgctt cgatacagca
      541 ggagccgaat ggctggaaga ccgtcaccac acagttctac atccaggaat tcagtgagca
      601 tattgcgcca cttcaggatg ctgtagatct ggagatcgca acggaggaag aaaactcgtt
      661 gctggaagcc tggaagaagt atcgggtgtt gctgaaccgt gttgatacat caactgcacc
      721 tgatattgag tggccggaag aaccagacac aa
//
LOCUS       DAEEKQ010000445          751 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.456, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000445 DAEEKQ010000000
VERSION     DAEEKQ010000445.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.456"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            584..>751
                     /locus_tag="KLK57_004582"
     CDS             584..>751
                     /locus_tag="KLK57_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBE0894728.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVID"
BASE COUNT          213 a          135 c          164 g          239 t
ORIGIN      
        1 cttgcttgca ccgtattgtt cttgtttttc ggttagttaa acatcgtact tcatatttga
       61 acgttctgcc ggaatgcatt atcaatagag gtaaagtcgc aaccccaaat cgtaaaggaa
      121 accgtagcac gtcgtatgca agaacgtgcc acggctggct gatggatgtt cgatagcgcg
      181 agtttgaatg aaaatcagcc ggagatgatt ttacataatt gctacggaat tattcaatac
      241 aggaattgct tgcgtctgca tggattgacc tgaaatattc ccgaaaattt ctctaaaaaa
      301 ctcgaaaaaa atggtaactg gttgaatgta ttaatatgca atggtacgtg tcagggatta
      361 aaagatgaac gtaaatttat ttaacgcatt aattttaaag ggttttattg tttgttgacg
      421 aaaacaggaa tcgtgttcgg tctcttttta tctgttaaaa gccagaagca tttccttcgc
      481 tgactttata gtcaaccata acacacactc tactgtctga gtccaacgtt ttttaacatt
      541 cttgttaaaa ttatgtgatc tttagcgcgg gaggaaaata ttgatgaaac agcctgcgcc
      601 cgtttatcag agaattgcgg gtcatcaatg gcgacatatc tggctttctg gcgatataca
      661 cggttgtctt gagcagttgc gccgcaaatt atggcattgt cgttttgatc cgtggcgaga
      721 tttacttatc tcagtgggag acgttatcga t
//
LOCUS       DAEEKQ010000446          751 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.457, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000446 DAEEKQ010000000
VERSION     DAEEKQ010000446.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.457"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            320..>751
                     /locus_tag="KLK57_004583"
     CDS             320..>751
                     /locus_tag="KLK57_004583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894729.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQQAILSAMSIYKFSITDDLMSMAVNELIKLT
                     KIENNVDLNKFTTICTDVLSPLVTRHNKEKKQTTFYPSQKSP"
BASE COUNT          299 a          118 c          122 g          212 t
ORIGIN      
        1 gtaacgtctc cataaggtga taaccttgat caaatgaatt tcgaatataa taaaggaaat
       61 cttttgagat gtttatcacg aataaagcta tttatgttac caatatcatt tcgagtgcgc
      121 acactatcta tctgtttttt cgagatataa aaaacaacgt gtaagttatg taaattgccg
      181 atgctactga gtaacattat ggtaaaaaaa gtaaaaatga gcgatttttg atagaataaa
      241 gcatgagaaa aggtgatatt tgacattatc agaagctgcg aattcggatt ttgctctaat
      301 caaatgagag aaatgtaaaa tgaagccacg aaatattaat aatagcctac cactgcaacc
      361 attagttcct gatcaggaga acaaaaataa gaaaaatgaa gagaaatccg ttaatccagt
      421 taaaatcaca atggggtctg gtttaaatta tattgaacaa gaatctcttg gaggaaaata
      481 tctaacacat gatttgtcaa taaagatagc ggatatttct gaagagataa ttcagcaagc
      541 aatattatct gctatgagca tatataaatt ttcgataaca gatgatttaa tgagtatggc
      601 tgtaaatgaa ctcataaaac tgaccaaaat agagaataat gtagacctga ataaattcac
      661 tactatatgc acagacgttc tatcccccct cgtcaccaga cataataaag aaaaaaaaca
      721 aacgacattc tacccttctc aaaaatcccc t
//
LOCUS       DAEEKQ010000447          751 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.458, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000447 DAEEKQ010000000
VERSION     DAEEKQ010000447.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.458"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>751
                     /locus_tag="KLK57_004584"
     CDS             <1..>751
                     /locus_tag="KLK57_004584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HBE0894730.1"
                     /translation="KRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPE
                     APVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQNSLARYADWLKDFKQ
                     GPTGKESTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTI
                     NEMDKYYTQENYKDDAFAKGKTLHQTFLKNLESFEPVAESYHAAIQEINDKRQLAELK
                     NIEEREGKTFHYYSLAVMISAKQINNLISQE"
BASE COUNT          240 a          160 c          176 g          175 t
ORIGIN      
        1 tgaaaaggaa tttattatct tccgctatta tagtcgccat catggccctc ggtctgacgg
       61 gttgtgatga taaaaaagcc gaaacagaaa cgcccccgcc tgccaatagt caacctgccg
      121 caccagctcc tgaagctccc gttgcaaaag cagaagctaa acctgaaaca cctgcgcaac
      181 cggtggtcga tgaacaagcg gttttcgacg aaaaaatgga tgtctatatc aagtgctaca
      241 acaagttaca gatccctgta cagaacagtc tggcgcgtta tgctgactgg ctgaaagatt
      301 ttaaacaggg gcctaccggt aaagagagta ctgtttatgg catctacggc attagtgaat
      361 ccaacctcgc tgagtgtgaa aaaggcgtaa aaagtgctgt ggcgttaacg cctgcgctgc
      421 aacctattga tggcgtggcg gtgagttata ttgatgctgc cgtggcgttg ggtaatacca
      481 ttaacgaaat ggataaatat tacacccagg aaaattataa agacgatgcg tttgcgaaag
      541 ggaaaacgct gcaccagaca ttcttaaaaa atctggaatc ctttgaacct gtagcagaat
      601 cttatcatgc ggcgattcag gaaattaatg ataagcgcca gcttgcggaa ctgaaaaata
      661 ttgaagaaag agaagggaaa acattccact actactctct ggcagtcatg atttcagcga
      721 aacaaattaa taacctgatt tcgcaagaga a
//
LOCUS       DAEEKQ010000448          746 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.459, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000448 DAEEKQ010000000
VERSION     DAEEKQ010000448.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 746)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..746
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.459"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            49..>746
                     /locus_tag="KLK57_004585"
     CDS             49..>746
                     /locus_tag="KLK57_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HBE0894731.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNAGVIVFKGSDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVY"
BASE COUNT          246 a          129 c          164 g          207 t
ORIGIN      
        1 ttaacgattt accagtaatg taaataaatt ttcgaggaga tcattccagt gggacgtaaa
       61 tgggccaata ttgttgctaa aaaaacggct aaagacggtg caacgtctaa aatttatgca
      121 aaattcggtg tagaaatcta tgctgctgct aaacaaggtg aacccgatcc agaattaaac
      181 acatctttaa aattcgttat tgaacgtgca aagcaggcac aagttccaaa gcacgttatt
      241 gataaagcaa ttgataaagc caaaggcggc ggagatgaaa cgttcgtgca gggacgttat
      301 gaaggctttg gtcctaatgg ctcaatgatt atcgccgaga cattgacttc aaatgttaac
      361 cgtacgattg ctaacgttcg cacaattttc aataaaaaag gcggcaatat cggagcggca
      421 ggttctgtca gctatatgtt tgacaatgct ggtgtgattg tatttaaagg atcagaccct
      481 gaccatattt ttgaaatttt acttgaagct gaagttgatg ttcgtgatgt gactgaagaa
      541 gaaggtaaca ttgttattta taccgaacct actgaccttc ataaaggaat cgcggctcta
      601 aaagcagctg gaatcactga gttctcaaca acagaattag aaatgattgc tcaatctgaa
      661 gttgagcttt ccccagaaga tttagaaatc tttgaagggc ttgttgatgc ccttgaagat
      721 gacgacgatg tacaaaaagt ttatca
//
LOCUS       DAEEKQ010000449          741 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.460, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000449 DAEEKQ010000000
VERSION     DAEEKQ010000449.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..741
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.460"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            176..694
                     /locus_tag="KLK57_004586"
     CDS             176..694
                     /locus_tag="KLK57_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HBE0894732.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
BASE COUNT          214 a          167 c          144 g          216 t
ORIGIN      
        1 cattacccac aatacgatta aattaattaa aaccccacaa ataaattaaa cataagattt
       61 tactcattgt gttttatttc tcacattgat gacgatcgca tgaaaaaatg atattttaat
      121 gagcattctg aaaaaatatc gatgcatttc gagcgaagat ggtggggatc cctgaatgcg
      181 tttttctcac cgactgttct tacttctgat tctcctgctg acgggagcac caatactcgc
      241 tcaggagcct tctgacgttg cgaaaaacgt gcgtatgatg gtttccggta ttgtcagtta
      301 cacccgctgg cctgcgctat cagggccacc aaagttatgt atcttttcat cttcacgttt
      361 tagtaccgcc ctacaagaga atgccgcgac atctttgcct tatctgcctg tcatcattca
      421 cactcaacaa gaggcgatga tttcaggctg caatggtttt tattttggca acgagtcacc
      481 gacattccag atggaattaa ccgaacaata tccgtcaaag gcgttattat taattgccga
      541 acaaaatacc gagtgcatta ttggtagcgc cttttgtctg atcatccaca ataatgacgt
      601 cagatttgcc gtaaacctgg atgccttatc gcgtagcggt gtaaaggtca acccggatgt
      661 cttaatgctc gcacggaaga aaaatgatgg ataacgataa ttctcttaat aagcgcccca
      721 cgtttaaaag agcattacgc a
//
LOCUS       DAEEKQ010000450          731 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.461, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000450 DAEEKQ010000000
VERSION     DAEEKQ010000450.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.461"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..241
                     /locus_tag="KLK57_004587"
     CDS             <1..241
                     /locus_tag="KLK57_004587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894733.1"
                     /translation="GVGLLTGNGVNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGA
                     AVGAVTGVLTGNGLEGAIKGAVIGGTGGAILGKMK"
     gene            245..580
                     /locus_tag="KLK57_004588"
     CDS             245..580
                     /locus_tag="KLK57_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HBE0894734.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
BASE COUNT          202 a          154 c          202 g          173 t
ORIGIN      
        1 aggcgttgga ttacttactg gcaatggtgt taatggcgta ctgaaaggtg cagctgttgg
       61 cgctggtgtt ggtgcagtaa cagaaaaagg ccgcgacggt aaaaatgcac gtaaaggtgc
      121 aaaagtgggt gccgccgttg gtgctgtcac tggcgttctg accggcaatg gcctcgaagg
      181 ggcgattaaa ggtgccgtga ttggcggtac gggtggtgca attctgggta aaatgaaata
      241 aggaatgatg atgaaaaaat ttgctttact ggcgggcctg tttgttttcg caccaatgac
      301 ctgggcgcag gactacaata ttaaaaacgg tttgccgtca gaaacctata ttacttgcgc
      361 agaagccaac gaaatggcaa aaactgacag cgcacaggtt gcagaaattg ttgcggtgat
      421 gggtaatgcc agcgttgcca gccgtgattt aaaaattgag caatctccgg aactgagcgc
      481 caaagtggta gaaaaactga atcaggtttg cgctaaagac ccgcaaatgc tgttgatcac
      541 ggcaattgat gacaccatgc gtgctatcgg caaaaaataa tatttaatat gttaccgcgt
      601 cgatgaattc gacgcggtat tttagctaac ggggatcaga acttatattt caggcccacc
      661 atcactgcgg tgtcattgta gccattatca cccagctgca cgcccacatt accccagaca
      721 ctggccgccg g
//
LOCUS       DAEEKQ010000451          731 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.462, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000451 DAEEKQ010000000
VERSION     DAEEKQ010000451.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.462"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..>731)
                     /locus_tag="KLK57_004589"
     CDS             complement(<1..>731)
                     /locus_tag="KLK57_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HBE0894735.1"
                     /translation="KAIMVNGPQFGWYAPAYTYGIGLHGAGYDVTGNTPFAYPGLVFG
                     HNGVISWGSTAGFGDDVDIFAERLLAEKPGYYLHNGKWVKMLSREETITVKNGQAETF
                     TVWRTVHGNILQTDQTTQTAYAKSRAWDGKEVASLLAWTHQMKAKNWQEWTQQAAKQA
                     LTINWYYADVNGNIGYVHTGAYPDRQSGHDPRLPVPGTGKWDWKGLLPFEMNPKVYNP
                     LSGYIANWNNSPQKDYPASDLFAFL"
BASE COUNT          194 a          195 c          155 g          187 t
ORIGIN      
        1 caaaaaggca aacagatccg aagcgggata atctttttgg ggagaattgt tccagttagc
       61 aatatatccc gacagggggt tatacacctt agggttcatt tcaaaaggca atagcccttt
      121 ccagtcccat tttcccgtac caggaacggg taatcgcgga tcatggcctg attgacgatc
      181 tggataagca ccagtatgaa cataaccaat attgccgttt acatcagcat agtaccagtt
      241 gatggtcagt gcttgtttcg ctgcctgctg tgtccactcc tgccaatttt tggccttcat
      301 ctgatgagtc caggccagca aagacgccac ctctttacca tcccatgcgc gggatttagc
      361 gtaagccgtt tgtgtcgtct ggtcagtttg gagaatgttg ccatgcaccg tacgccagac
      421 agtaaaggtc tctgcctgac catttttcac cgtaatggtt tcctcacggc ttaacatttt
      481 cacccactta ccattatgca agtagtagcc tggtttctct gccaacagcc gttcagcaaa
      541 aatatcgaca tcatcgccga aacctgccgt tgatccccag gaaatcacac cattatgacc
      601 aaaaaccagc ccaggatagg caaatggtgt attgccagtg acatcataac cagcaccgtg
      661 cagaccaata ccataagtat acgcaggcgc ataccagcca aactgcggac catttaccat
      721 gattgctttc g
//
LOCUS       DAEEKQ010000452          731 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.463, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000452 DAEEKQ010000000
VERSION     DAEEKQ010000452.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.463"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(162..>731)
                     /locus_tag="KLK57_004590"
     CDS             complement(162..>731)
                     /locus_tag="KLK57_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0894736.1"
                     /translation="FFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIV
                     IAGILLNVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFF
                     IAVVLGMIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQ
                     IHLLDKYRKVPPEPVHADIPVGAVSDVKA"
BASE COUNT          226 a          180 c          158 g          167 t
ORIGIN      
        1 gctttggctt gataaccgga taacaacgtg cgctttgttg atgccggatg cggcgtgaat
       61 tccttatccg gtctacaaaa tttgcaaatc caaagcatta cattaagacg tggcctgata
      121 aacatatcgc atcaggccat tttcgttagc tattgtccac gtcaggcttt cacatcactc
      181 actgcaccga ccggaatatc agcatgaaca ggctcgggcg gtactttgcg atatttatcc
      241 agcagatgaa tctggatcct gcgcagcgtg tcgccattga ggcgatagaa acggaagtag
      301 agaatcagcg ttaccatgaa aaacagagtt ggtagagcaa tcataataaa ctgcatacct
      361 aatagggctt gcgtagactg ttcaacattc ggtacatagc caatcatccc taacacaacc
      421 gcaatgaaaa aagccgcaaa ggctgaaccg cccttcacca ccatagtctg cacggagtaa
      481 gcgatacttt cacagcgtac gtgcagttta tattcaccgt aatcaacggt atctgccacc
      541 atgatgacct gtaataccca gaaaagcgcc gttcccacat tcagcaaaat acccgcaatc
      601 acaatgagga cgacgttgtg atagctcatt aatgccatta acaggagaac accacagctt
      661 aacaccggaa gaatagatgc tccggcccat aaaatgcgtc gggataatga tttaactaag
      721 cgtgggaaga a
//
LOCUS       DAEEKQ010000453          728 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.464, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000453 DAEEKQ010000000
VERSION     DAEEKQ010000453.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.464"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>728
                     /locus_tag="KLK57_004591"
     CDS             <1..>728
                     /locus_tag="KLK57_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HBE0894737.1"
                     /translation="IEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGETKLYVPQGV
                     QAYPTLRPLIGGTLNIKHVRAHWDDILRLASSIKQGTVTASLMLRKLGSYPRQNGLAV
                     ALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNSLARAVFFNRLGEIRDRSFE
                     QQRYRASGLNLVTAAIVLWNTVYLERATQGLVEAGKPVDGELLQFLSPLGWEHINLTG
                     DYVWRQSRRLEDGKFRPLRMPGKP"
BASE COUNT          133 a          242 c          236 g          117 t
ORIGIN      
        1 atcgaggagc actacaccga cacggccggc ttcaccgatc acgtctttgc cctgatgcac
       61 ctgctaggct tccgcttcgc gccgcgcatc cgcgacctcg gcgaaaccaa gctgtacgtg
      121 ccgcagggcg tgcaagccta cccgacgttg cgcccgctga tcggcggcac cctgaacatc
      181 aagcacgtgc gtgcccactg ggacgacatc ctgcgcctgg ccagctcgat caagcagggc
      241 accgtcaccg cctcgctgat gctgcgcaag ctcggcagct acccgcgcca gaacggactg
      301 gccgtggccc tgcgcgagct gggccggatc gagcgcacgc tgttcatcct ggactggctg
      361 caaagtgttg aactgcgccg ccgcgtgcat gccggcctga acaaaggtga ggcgcgcaac
      421 tcgctggcca gggcggtgtt cttcaaccgc cttggggaaa tcagggatcg gagcttcgag
      481 cagcagcgct accgggccag cggcctcaac ctggtgacgg cggctatcgt gctgtggaac
      541 acggtgtacc tggaacgcgc cacccagggg ttggtcgagg ccggcaagcc ggtggacggc
      601 gagctgctgc aattcctgtc gccgctgggc tgggagcaca tcaacctaac cggcgattac
      661 gtctggcggc agagccgcag actggaagac gggaagtttc ggcccttacg gatgcccgga
      721 aaacctta
//
LOCUS       DAEEKQ010000454          728 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.465, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000454 DAEEKQ010000000
VERSION     DAEEKQ010000454.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..728
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.465"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..443
                     /locus_tag="KLK57_004592"
     CDS             <1..443
                     /locus_tag="KLK57_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0894738.1"
                     /translation="VSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSIL
                     DQGWYEMHRQLEYKQLWRGGQVLAVPPAYTSQRCAYCGHTAKENRLSQSKFRCQVCGY
                     TANADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
     gene            complement(483..>728)
                     /locus_tag="KLK57_004593"
     CDS             complement(483..>728)
                     /locus_tag="KLK57_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HBE0894739.1"
                     /translation="AALPVSWIQMLAGLALLSTIGGSLYQALHNERERERDAAVVAFL
                     VTASGLTLVGIGSAFWGLIAGGVCYVVLNLIADRNRY"
BASE COUNT          190 a          204 c          195 g          139 t
ORIGIN      
        1 ccgtcagcaa aaaccacgca atgatagtca ttgaggattt gaaggtcagc aacatgtcaa
       61 agtcagcagc gggtacggtc agccagccgg ggcgcaatgt ccgggcaaaa tcaggtttaa
      121 accgttcgat actggatcag ggctggtatg aaatgcaccg ccagcttgag tacaagcagc
      181 tctggcgtgg cggtcaggtg cttgctgttc cgccagcgta tacaagccag cgttgcgcgt
      241 actgtggtca tacagcgaaa gagaaccgcc tgtcacaaag taaattcaga tgccaggtat
      301 gtggatatac agcgaacgcc gatgtaaatg gtgctcgtaa cattttagcg gcggggcacg
      361 ccgttcttgc ctgtggagag atggtgcagt caggccgccc gttgaagcag gaacccaccg
      421 aaatgattca ggcgacagcc tgaacgtagc agggatccac gtccttcagg gcgtggagga
      481 tgtcaatatc ggtttctgtc agcgattaaa ttcaacacca cgtaacaaac gcctccggca
      541 attaatcccc agaacgcaga accaatcccg accagcgtca atccacttgc cgttaccaga
      601 aatgccacca ccgccgcgtc gcgctcacgc tcacgctcat tatgcagcgc ctgatacaaa
      661 ctgccgccaa tggtactcaa cagcgccaga cctgccagca tctggatcca acttacgggc
      721 agggcagc
//
LOCUS       DAEEKQ010000455          694 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.466, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000455 DAEEKQ010000000
VERSION     DAEEKQ010000455.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 694)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 694)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..694
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.466"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            136..>694
                     /locus_tag="KLK57_004594"
     CDS             136..>694
                     /locus_tag="KLK57_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894740.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFI"
BASE COUNT          204 a          161 c          154 g          175 t
ORIGIN      
        1 atttcggcgg gaacaccctc cccgccgaac ttaaaaaata tatttaaccg tatttaataa
       61 aaatatttcg tgagtctctg tgcgctaatt ctccatctgg cgtagggaaa atcacatctg
      121 aatcaggaat taacaatgaa acctgtaaaa ccacctcgta ttaatggacg agtgccggtc
      181 ctgtcggcac aggaagcggt gaattatatt cccgacgaag caacactttg tgtgttaggc
      241 gctggcggcg gtattctgga agccaccacg ttaattacag ctcttgctga taaatataaa
      301 cagactcaaa cgccacgtaa tttatcgatt attagtccga cagggcttgg cgatcgcgcc
      361 gaccgtggta ttagtcctct ggcgcaagaa ggtctggtga aatgggcatt atgtggtcac
      421 tggggacaat cgccgcgtat ttctgaactc gcagaacaaa ataaaattat tgcttataac
      481 tatccacaag gtgtacttac acaaacctta cgcgccgccg cagcccacca gcctggtatt
      541 attagtgata ttggcatcgg gacatttgtc gatccacgcc agcaaggcgg caaactgaat
      601 gaagtcacga aagaagacct gattaaactg gtcgagtttg ataacaaaga atatctctat
      661 tacaaagcga ttgcgccaga tatcgccttc attc
//
LOCUS       DAEEKQ010000456          692 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.467, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000456 DAEEKQ010000000
VERSION     DAEEKQ010000456.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.467"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>692
                     /gene="tuf"
                     /locus_tag="KLK57_004595"
     CDS             <1..>692
                     /gene="tuf"
                     /locus_tag="KLK57_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HBE0894741.1"
                     /translation="VDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHILLG
                     RQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKALEG
                     DAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGIIKV
                     GEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAKPG
                     TIKPHTKFESEV"
BASE COUNT          161 a          166 c          205 g          160 t
ORIGIN      
        1 gtagactgcc cggggcacgc cgactatgtt aaaaacatga tcaccggtgc tgctcagatg
       61 gacggcgcga tcctggtagt tgctgcgact gacggcccga tgccgcagac tcgtgagcac
      121 atcctgctgg gtcgtcaggt aggcgttccg tacatcatcg tgttcctgaa caaatgcgac
      181 atggttgatg acgaagagct gctggaactg gttgaaatgg aagttcgtga acttctgtct
      241 cagtacgact tcccgggcga cgacactccg atcgttcgtg gttctgctct gaaagcgctg
      301 gaaggcgacg cagagtggga agcgaaaatc ctggaactgg ctggcttcct ggattcttac
      361 attccggaac cagagcgtgc gattgacaag ccgttcctgc tgccgatcga agacgtattc
      421 tccatctccg gtcgtggtac cgttgttacc ggtcgtgtag aacgcggtat catcaaagtt
      481 ggtgaagaag ttgaaatcgt tggtatcaaa gagactcaga agtctacctg tactggcgtt
      541 gaaatgttcc gcaaactgct ggacgaaggc cgtgctggtg agaacgtagg tgttctgctg
      601 cgtggtatca aacgtgaaga aatcgaacgt ggtcaggtac tggctaagcc gggcaccatc
      661 aagccgcaca ccaagttcga atctgaagtg ta
//
LOCUS       DAEEKQ010000457          677 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.468, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000457 DAEEKQ010000000
VERSION     DAEEKQ010000457.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..677
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.468"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..361
                     /locus_tag="KLK57_004596"
                     /pseudo
     CDS             1..361
                     /locus_tag="KLK57_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415229.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          194 a          170 c          171 g          142 t
ORIGIN      
        1 gcagatgcaa agccagatgg acccggtata cacgccggcg cgaaaaattc acctgtacca
       61 ctgcgaccat cgcggcctgc cgctggccct tatcagcacg gaagggacaa cagcgtggtg
      121 cgcagaatat gatgaatggg gcaacctgct gagtgatgag aacccgcatc atctgcagca
      181 gctcattcgc ctgccggggc agcagtatga tgaggagtcc ggcctgtatt acaaccgcca
      241 ccgctattat gacccgctgc aggggcggta tatcactcag gatccgattg gactgaaggg
      301 gggatggaat ttttatcagt atccgttgaa tccggttcag tatatagatt caatggggct
      361 ggcatctaaa tatggacact taaataatgg cggatatgga gcgaaaccca gcaaaccgcc
      421 tacgcccgat ccaagtaagt tgccagacat agcgaaacaa ttaagactgc catatcctat
      481 tgaccaggcc agtagtgcgc ctaatgtttt caaaacattc ttcagagcat taagccctta
      541 cgattacaca ctgtattgca ggaagtgggt aaaacccaat ctgacttgta tgccgcagga
      601 tgatccccag tatccaggga tggagacaaa gacagcaagt gattacctgc cacagacaaa
      661 ttggtcaaca actcaat
//
LOCUS       DAEEKQ010000458          675 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.469, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000458 DAEEKQ010000000
VERSION     DAEEKQ010000458.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..675
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.469"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>675
                     /locus_tag="KLK57_004597"
     CDS             <1..>675
                     /locus_tag="KLK57_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HBE0894742.1"
                     /translation="DVLLRIHHVIGELPTYGYRRVWALLRRQAELDGMPAINAKRVYR
                     IMRQNALLLERKPAVPPSKRAHTGRVAVKESNQRWCSDGFEFCCDNGERLRVTFALDC
                     CDREALHWAVTTGGFNSETVQDVMLGAVERRFGNDLPSSPVEWLTDNGSCYRANETRQ
                     FARMLGLEPKNTAVRSPESNGIAESFVKTIKRDYISIMPKPDGLTAAKNLAEAFEHYN
                     EWHPHSA"
BASE COUNT          166 a          162 c          208 g          139 t
ORIGIN      
        1 ggatgtgctt ctccgtatac accatgttat cggagagctg cccacgtatg gttatcgtcg
       61 ggtatgggcg ctgcttcgca gacaggcaga acttgatggt atgcctgcga tcaatgccaa
      121 acgtgtttac cggatcatgc gccagaatgc gctgttgctt gagcgaaaac ctgctgtacc
      181 gccatcgaaa cgggcacata caggcagagt ggccgtgaaa gaaagcaatc agcgatggtg
      241 ctctgacggg ttcgagttct gctgtgataa cggagagaga ctgcgtgtca cgttcgcgct
      301 ggactgctgt gatcgtgagg cactgcactg ggcggtgact accggcggct tcaacagtga
      361 aacagtacag gacgtcatgc tgggagcggt ggaacgccgc ttcggcaacg atcttccgtc
      421 gtctccagtg gagtggctga cggataatgg ttcatgctac cgggctaatg aaacacgcca
      481 gttcgcccgg atgttgggac ttgaaccgaa gaacacggcg gtgcggagtc cggagagtaa
      541 cggaatagca gagagcttcg tgaaaacgat aaagcgtgac tacatcagta tcatgcccaa
      601 accagacggg ttaacggcag caaagaacct tgcagaggcg ttcgagcatt ataacgaatg
      661 gcatccgcat agtgc
//
LOCUS       DAEEKQ010000459          671 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.470, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000459 DAEEKQ010000000
VERSION     DAEEKQ010000459.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..671
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.470"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..649
                     /locus_tag="KLK57_004598"
     CDS             <1..649
                     /locus_tag="KLK57_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element IS600 family transposase"
                     /protein_id="HBE0894743.1"
                     /translation="GIIVGRDRLARLRKELRLRCKQKRKFRATTNSNHNLPVAPNLLN
                     QTFAPTAPNQVWVADLTYVATQEGWLYLAGIKDVYTCEIVGYAMGERMTKELTGKALF
                     MALRSQRPPAGLIHHSDRGSQYCAYDYRVIQEQSGLKTSMSRKGNCYDNAPMESFWGT
                     LKNESLSHYRFNNRDEAISVIREYIEIFYNRQRRHSRLGNISPAAFREKYHQMAA"
BASE COUNT          185 a          166 c          174 g          146 t
ORIGIN      
        1 tggcatcatc gttggtcgtg accgactggc acgtcttcgt aaggagctaa ggctacgctg
       61 taagcagaaa cgcaagttca gagcgactac gaactcgaac cacaatctgc cagttgcgcc
      121 aaatctgctg aaccagacgt tcgctcctac agcaccaaat caggtctggg tggcggacct
      181 gacgtatgtt gccacacagg agggatggtt gtacctcgct ggcatcaaag atgtttatac
      241 gtgcgaaatt gtcggctacg ccatgggaga gcgcatgaca aaagagctga caggtaaagc
      301 cctgtttatg gcgctcagga gccagcgccc acctgccggg ctaatccacc actctgatcg
      361 aggttcacag tactgcgcat acgattaccg ggtcatacag gagcagtctg gtctgaaaac
      421 atcaatgtcg cgtaaaggta actgttacga caacgctccg atggaaagct tctggggaac
      481 gctgaaaaat gagagcctga gccactatcg ttttaataac cgggatgaag ccatctcagt
      541 aatacgggaa tacattgaga ttttctacaa tcgtcagcgt cgtcactctc gtctggggaa
      601 tatctccccg gcagccttca gggaaaaata tcatcagatg gctgcttaaa aaaagaacaa
      661 atggtagtgt c
//
LOCUS       DAEEKQ010000460          664 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.471, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000460 DAEEKQ010000000
VERSION     DAEEKQ010000460.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..664
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.471"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>664
                     /locus_tag="KLK57_004599"
     CDS             <1..>664
                     /locus_tag="KLK57_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HBE0894744.1"
                     /translation="LPQRSDTLDKQELNLKSDRKEKEFPRIKLNGQCYFPGRPQNRIV
                     CRHIAAQYINDIYQNVDYKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAA
                     CGDFGLVMKAYFDKMESNGISVMAAILLVDNHALTVRLRIKNTTEGCTHYVVSVYDPN
                     VTNDKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLIL
                     KGS"
BASE COUNT          230 a          125 c          123 g          186 t
ORIGIN      
        1 ttttacctca acgctcagac actcttgata aacaagaatt aaacctaaaa tcagatagaa
       61 aagaaaaaga attcccaaga attaaactta atggtcaatg ttattttccg gggcgacccc
      121 aaaaccgtat agtatgccga cacattgctg cacaatatat taatgatatt tatcagaatg
      181 ttgattacaa accccatcaa gatgattatt cttcagctga aaaatttctc actcacttca
      241 acaaaaaatg caaaaaccag actttggcgt tgatttccag ccgtcctgag gggcgttgcg
      301 ttgctgcctg cggtgatttc gggctagtta tgaaagcata ttttgacaag atggaatcaa
      361 atggcatcag tgttatggca gccatattac tggtggataa ccatgctttg acggtccggc
      421 taagaataaa gaacacaact gaaggatgta cccattacgt ggtttcggtt tatgatccta
      481 atgtaactaa cgacaaaata agaatcatga gcgaaagcaa agaggatatt aaacactatt
      541 ctctgatgga ttttatgaat gtagattata gcctcctgaa atggtcaaat gatcatgtta
      601 ttaaccaatc tgttgcaata attccagcac ttccgaaaga acagctattg atattaaaag
      661 gatc
//
LOCUS       DAEEKQ010000461          663 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.472, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000461 DAEEKQ010000000
VERSION     DAEEKQ010000461.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.472"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>663
                     /locus_tag="KLK57_004600"
     CDS             <1..>663
                     /locus_tag="KLK57_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HBE0894745.1"
                     /translation="DENTIGVVPTFGVTYTGNYEFPQPLHDALDKFQADTGIDIDMHI
                     DAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFGLAPLGCGWVIWRDEEALPQELV
                     FNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGREGYTKVQNASYQVAAYLADEIA
                     KLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEAT
                     DIV"
BASE COUNT          132 a          194 c          189 g          148 t
ORIGIN      
        1 gacgaaaaca ccatcggcgt ggtgccgact ttcggcgtga cctacaccgg taactatgag
       61 ttcccacaac cgctgcacga tgcgctggat aaattccagg ccgacaccgg tatcgacatc
      121 gacatgcaca tcgacgctgc cagcggtggc ttcctggcac cgttcgtcgc cccggatatc
      181 gtctgggact tccgcctgcc gcgtgtgaaa tcgatcagtg cttcaggcca taaattcggt
      241 ctggctccgc tgggctgcgg ctgggttatc tggcgtgacg aagaagcgct gccgcaggaa
      301 ctggtgttca acgttgacta cctgggtggt caaattggta cttttgccat caacttctcc
      361 cgcccggcgg gtcaggtaat tgcacagtac tatgaattcc tgcgcctcgg tcgtgaaggc
      421 tataccaaag tacagaacgc ctcttaccag gttgccgctt atctggcgga tgaaatcgcc
      481 aaactggggc cgtatgagtt catctgtacg ggtcgcccgg acgaaggcat cccggcggtt
      541 tgcttcaaac tgaaagatgg tgaagatccg ggatacaccc tgtacgacct ctctgaacgt
      601 ctgcgtctgc gcggctggca ggttccggcc ttcactctcg gcggtgaagc caccgacatc
      661 gtg
//
LOCUS       DAEEKQ010000462          649 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.473, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000462 DAEEKQ010000000
VERSION     DAEEKQ010000462.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..649
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.473"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..196
                     /locus_tag="KLK57_004601"
     CDS             <1..196
                     /locus_tag="KLK57_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine-protein phosphatase"
                     /protein_id="HBE0894746.1"
                     /translation="DVDLHQVLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGN
                     LHYIDTGAVFGGELTLVQLQ"
     gene            complement(197..388)
                     /locus_tag="KLK57_004602"
     CDS             complement(197..388)
                     /locus_tag="KLK57_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HBE0894747.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(493..>649)
                     /locus_tag="KLK57_004603"
     CDS             complement(493..>649)
                     /locus_tag="KLK57_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HBE0894748.1"
                     /translation="TIPCKSDPDLCMQLDAWDAETSIPALLNGEHSVLYRTRYDQQSD
                     AWIMRLA"
BASE COUNT          182 a          147 c          162 g          158 t
ORIGIN      
        1 ggacgttgat ttgcatcagg tcttgtggag ccgctcgcga ttaggtgaac gccaaaaagg
       61 gcagggaatt acaggtgctg atcatttctg gtttggtcat acaccgttgc gacatcgcgt
      121 ggatattggc aacctgcatt atattgatac cggtgctgtc tttgggggcg aactgactct
      181 tgtgcaattg caataattaa aaatcaccgt actcctgtgc aggtcgccag aaaccatcta
      241 taaaatcctc aatcggaaaa caaccgccat ggcggatccg ttgatcgctc atagaataaa
      301 gacactgctg ttccgtgttg tagacatcca caacaatatc ttcacaaccg ccatccaggt
      361 agcaaacaaa aagtaccagc gcgaacattt catccccgaa gtgtggtgcc gtaccgttaa
      421 gtttaggaga gattttacaa cgggggaata accaggacaa ataacccgcc atcccgacgg
      481 gttctttttg gatcaggcaa gacgcataat ccaggcatca gattgttgat cgtaacgggt
      541 acggtaaagg acagagtgtt cgccatttag cagggcaggg atactggttt cagcatccca
      601 ggcatcgagt tgcatacata aatcggggtc agatttacaa ggaatggtt
//
LOCUS       DAEEKQ010000463          643 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.474, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000463 DAEEKQ010000000
VERSION     DAEEKQ010000463.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.474"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>643
                     /gene="fliD"
                     /locus_tag="KLK57_004604"
     CDS             <1..>643
                     /gene="fliD"
                     /locus_tag="KLK57_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HBE0894749.1"
                     /translation="KVSTEAGAAPGIYTISVTQLAQAQSLRTDSPTIIASTKDALGDE
                     SSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISKAINDADSGISASIVKVKDGNYQLV
                     LTASEGLANKMTISVEGDSKLNDLLAYDSKTNTGNMKELVNAQNAQLNVNGIDIERSS
                     NKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATEAIKGWVDSYNSLIDTFNTLT"
BASE COUNT          219 a          146 c          139 g          139 t
ORIGIN      
        1 aaagtttcca ccgaagcagg tgctgccccc ggtatctata ccatcagcgt aacccaactg
       61 gcgcaggcgc aatctttacg aactgattcc ccgacaatca ttgcatcaac aaaagatgca
      121 ctgggtgatg agtcatcaga tacacgcaca ataaaaatta cccaagacgg tcgtaaagag
      181 ccgctggaaa tcaagctcaa taaagatcag acgtcattgg atgagatcag caaagccatt
      241 aacgatgccg atagtggaat ttccgctagt atcgttaaag tcaaagatgg taactatcaa
      301 ttggtattga ccgccagtga aggtctggcc aataaaatga ccatatctgt tgagggtgac
      361 agtaaattaa atgatctgct ggcttatgac agcaagacca acaccgggaa tatgaaggaa
      421 ctggttaacg cgcaaaatgc acagctaaac gttaacggta tcgatattga acgttcaagt
      481 aacaaaatta ccgatgctcc tcagggagtg actctcgatt tgaccaaaaa agtcactgac
      541 gtccgggtca cggtgacaaa aagcaacgat aaagcaaccg aagcaattaa aggctgggtg
      601 gattcatata actccctcat tgacaccttt aacaccctga caa
//
LOCUS       DAEEKQ010000464          639 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.475, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000464 DAEEKQ010000000
VERSION     DAEEKQ010000464.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.475"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..330
                     /locus_tag="KLK57_004605"
     CDS             <1..330
                     /locus_tag="KLK57_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HBE0894750.1"
                     /translation="KPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDTDIQN
                     NPQKTRPFNIQHSTFNIQHSTFNIQHSTFNIQHSTFNKERNLSLAWTSTLMMRLATTN
                     NGFSTKG"
     gene            308..>639
                     /gene="yhaV"
                     /locus_tag="KLK57_004606"
     CDS             308..>639
                     /gene="yhaV"
                     /locus_tag="KLK57_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HBE0894751.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRLFFRYSEK
                     EKVIILGW"
BASE COUNT          207 a          131 c          136 g          165 t
ORIGIN      
        1 aagccaggcc aggacagcat tcattacgaa attctgcctg gtgggcaagt atttatgtgc
       61 cgactgggag atgaacagga ggatcatact atgaatgcat ttttgcgttt tctggataca
      121 gatatccaga acaacccgca aaaaactcgt ccattcaaca ttcaacattc aacattcaac
      181 attcaacatt caacattcaa cattcaacat tcaacattca acattcaaca ttcaacattc
      241 aacaaggaaa gaaacttgtc actggcatgg acgtcaacat tgatgatgag attggcgacg
      301 acgaataatg gattttccac aaagggttaa tggttgggcg ctatatgctc atccctgttt
      361 tcaggaaacc tacgacgctt tagttgccga agtcgagaca ttaaagggaa aagatcctga
      421 aaattatcag agaaaagccg ccacaaagtt attggcggta gtccataaag tgattgagga
      481 gcatatcacg gtcaatccat catcaccggc attccgtcat ggcaagtcgt taggctctgg
      541 gaaaaataaa gactggtcac gggtaaaatt tggtgcaggt cgttatcgtc tcttctttcg
      601 ttatagtgaa aaagagaaag tcatcattct gggatggat
//
LOCUS       DAEEKQ010000465          639 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.476, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000465 DAEEKQ010000000
VERSION     DAEEKQ010000465.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.476"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(19..369)
                     /gene="traA"
                     /locus_tag="KLK57_004607"
                     /pseudo
     CDS             complement(19..369)
                     /gene="traA"
                     /locus_tag="KLK57_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673167.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system pilin TraA"
     gene            complement(415..>639)
                     /locus_tag="KLK57_004608"
     CDS             complement(415..>639)
                     /locus_tag="KLK57_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0894752.1"
                     /translation="DSYDNAMAEIIKGLYKAEVIHRKSWKNRTEVELATLTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
BASE COUNT          164 a          173 c          137 g          165 t
ORIGIN      
        1 acatcgtttt atttcctgtc agaatccggc aacagacata cctactgcaa taaatacaga
       61 gagtatggca aagccgacca ggaacttgac gtttttggtc atcatgtaca tgactgcacc
      121 gaccagaact tcagccagaa caacccattt aacgatactg gagtccttac cgaaggtcgc
      181 cttaaccgtt tcctgacctt tagccatcag gtccgtgcct ttaccagcag ccatagctaa
      241 ctcagggaac aacatcatca gaacagcaac cgggatcact gcacgaacca ggcgaagcat
      301 attcagacga gtgaatttag aaaaaaacga tttctttttg acgggcgcag aagtaccctg
      361 aacacttaac gctgaaccgc cccgggtttc ctggagagtg ttttatctgt gaactcaggc
      421 tgccagatca tcgtttccga tggaagcata ataagctttt tctgcttctg ccggaggagt
      481 atggcccagc ctttccagca atcgtcgatt gttataccag tccacccacg tgagtgtggc
      541 cagctccact tctgtccggt ttttccagct cttacggtgt attacctccg ctttgtaaag
      601 gcccttgatg atctctgcca tcgcgttgtc atacgagtc
//
LOCUS       DAEEKQ010000466          633 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.477, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000466 DAEEKQ010000000
VERSION     DAEEKQ010000466.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..633
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.477"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..100)
                     /locus_tag="KLK57_004609"
     CDS             complement(<1..100)
                     /locus_tag="KLK57_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HBE0894753.1"
                     /translation="MASVSISYPSCSATDGVVRNCKSTAGHQRYLCS"
     gene            156..633
                     /locus_tag="KLK57_004610"
                     /pseudo
     CDS             156..633
                     /locus_tag="KLK57_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
BASE COUNT          155 a          140 c          148 g          190 t
ORIGIN      
        1 gagagcagag atagcgctga tgtccggcag tgcttttgca gttacgcacc accccgtcag
       61 tagctgaaca ggagggatag ctgatagaaa cagaagccac tggagcgcct caaaaacacc
      121 atcatacact aaatcagtaa gttggcagca tcaccgccgt tgtctacaca tttattctct
      181 cggtgctgat ttaccgggaa gtaaaatggc gccatttacc caaactgatt ctggagtcgg
      241 ttgttaccac ttcgattgta ttactgttga tcggtttttc cgtgggcatg tcctgggcaa
      301 tgactaatgc tgatattcct tatatgatca gcgacgcatt gatggggatt tctgataacc
      361 cactgatcat attgctgctg attaatatcg tcttattaat tgtcggcatc tttatggaca
      421 tgacaccggc tgtactaatc tttacaccga tcttcttgcc gattgcacaa gagctgggaa
      481 tggacccagt acatttcggt attatgatgg tggccaacct ttgtattggg ctgttaacac
      541 cgccggtggg tagtgcgcta tttgttggtt gttctatctc gggtgtgaag attcaacatt
      601 taatcaaacc attgctgcca ttttatgcta gca
//
LOCUS       DAEEKQ010000467          626 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.478, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000467 DAEEKQ010000000
VERSION     DAEEKQ010000467.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..626
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.478"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
BASE COUNT          213 a           99 c          131 g          183 t
ORIGIN      
        1 tctgtaacga gctaaactgt ataagtggca gtaagatgcc ttttagactg acactcgctt
       61 agagagcttc ctagagcttc tgggaggatt cattagatat cgttgtatac agagacacag
      121 tatctataaa gggtattaag agattcttag aagttactgt aaagaaccac taagataaga
      181 agagagggac aaagaaccga aggaaagtcc cttattaagt aggagtctta atagactgtc
      241 ttaatagact gtcttaatag ataacttaaa agccttaaag gatatcttcc aagaagagag
      301 gaacgaagaa ccagaggaag tgaccgttga ccgtttgaac agactgttta agtaaagcct
      361 ataaagtatc tattaattaa gggctatgtc tcaatactag cagtttgtca atacctttgt
      421 ctttagcaat tcgtgctatt tattaaatat agcacttttt gttaaaacag agtatgcttt
      481 ttaacctaaa accttgtcgc ttcgctctgg gtcacatgtt cttgggttct tagtgagagt
      541 tacactatct tctaaggtac tcaataaaaa gacgagtaac aaccgaagaa cgaggataga
      601 ggtattgtaa taacttagaa ggtgtc
//
LOCUS       DAEEKQ010000468          611 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.479, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000468 DAEEKQ010000000
VERSION     DAEEKQ010000468.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..611
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.479"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            284..388
                     /locus_tag="KLK57_004611"
                     /pseudo
     CDS             284..388
                     /locus_tag="KLK57_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          206 a          118 c          121 g          166 t
ORIGIN      
        1 atcatacaga taattactgg ttaaccctat atagggcatt ttaaagtgtt gtgatcttac
       61 ccagcaatag tggacacgcg gctaagtgag taaactctca gtcagaggtg actcacaaga
      121 caaaacagtt tcaaccagta aaaaaacgta aacaccattc gcatgaattt cgtagttaag
      181 atctgaagct tgcagaatgc atcggtgtta ctgccggagt ccgtgaactt agtctgcatg
      241 aatcaccaca gctttacatc tgtcgcagta aacagcaaaa tcagcaaact tacttaatta
      301 tattgagtgc gattacaatc gctggcgatg gtacagttgg tgtggcggtc tcagtccgga
      361 acaatttgaa aatcagaatc tcacttaaag cggcatccat tttacaaaag atggccgtct
      421 gtgtatttgt aatgaatacc ttctacatgt ttagagtgca taatccacga caaggcgata
      481 tcctaaaaat tagctagcag gcaactttga tggcatagat aatgttttta gtaaaaaaga
      541 gttgactgaa tataatacag cctcttccta cataagaagg aatgtgctca caaaaaaaaa
      601 ccattagtct g
//
LOCUS       DAEEKQ010000469          596 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.480, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000469 DAEEKQ010000000
VERSION     DAEEKQ010000469.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.480"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            289..>596
                     /gene="mcbR"
                     /locus_tag="KLK57_004612"
     CDS             289..>596
                     /gene="mcbR"
                     /locus_tag="KLK57_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HBE0894754.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     "
BASE COUNT          175 a          121 c          149 g          151 t
ORIGIN      
        1 cggtttagag aatgcgccac agacgtttat cggcctgctg aagggtaaaa acttcggcaa
       61 agtggtgatc cgcgtcgcgg gtgatgatta aagtttcaac ggcggcgtaa gccgccataa
      121 aaatccaggc aaaaagctta ttttcagctt taattaacta accacgtcat tgttctgctg
      181 ctaataacaa caaaggtggg tggtttatga ttattcactt taatacacca ggtgaactcc
      241 ttctgccgtg caggcagggt tggacagaaa acacgcgaag gaattaaaat gcctggaacg
      301 ggaaaaatga aacatgtcag tttgactctg caggttgaga acgacctgaa acatcagctt
      361 agtattggcg cattaaaacc tggcgcacgc ctgattacta aaaatctggc ggagcaatta
      421 ggtatgagta ttacacctgt gcgtgaagca ttattacgtc tggtttcggt gaatgcgctt
      481 tctgtcgcac ctgcacaagc atttacagtt ccggaagtgg ggaaacgtca attagacgaa
      541 atcaatcgga tccgctacga actggaatta atggcagttg ctctggctgt tgaaaa
//
LOCUS       DAEEKQ010000470          582 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.481, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000470 DAEEKQ010000000
VERSION     DAEEKQ010000470.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..582
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.481"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            59..>582
                     /locus_tag="KLK57_004613"
     CDS             59..>582
                     /locus_tag="KLK57_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HBE0894755.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSTPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQR"
BASE COUNT          183 a          110 c          114 g          175 t
ORIGIN      
        1 aaatctacat ctctaataca ccaacttttt ggaaacaata ttgtaagatc aaatttatat
       61 ggtggcgaaa attaatctct tatcacggct tacgccgctt ttattcgtgt tcgctccttt
      121 tttggcacag tcaaatatga ctgtttatcc tatggctgtt tccattaata gtcagggtga
      181 aggcaacgtt cgcgtaatat ctaaaagtaa tgaagttcag tacatcaagg cgacagtatt
      241 ccgtatcgat aatccatcga cgcctcagga aaatgaagtt gagattaaat ccggtgatgc
      301 aaatcatctg gttgttatgc cacctaaatt cgctttacca gccggtagta gcaaaactgt
      361 gcgctttgtt gcaatggaac cagagcaaaa agaaaaaaat tatcgcgtta aatttgaagc
      421 ggttcccagt attgatgacg ttgccacaga taaaaaagat ctctctatgc agttaacagt
      481 taacttaatt tgggggattg ttgttagtgt tccacctcag caacctattg ctaagttaga
      541 agtaaatgct gcccaaaaat tagttaatgc agggaatcaa cg
//
LOCUS       DAEEKQ010000471          566 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.483, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000471 DAEEKQ010000000
VERSION     DAEEKQ010000471.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.483"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..225
                     /locus_tag="KLK57_004614"
     CDS             <1..225
                     /locus_tag="KLK57_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0894756.1"
                     /translation="DSYDNAMAESINGLYKAEVIHRKSWKNRAEVELAILTWVDWYNN
                     RRLLERLGHTPPAEAEKAYYASIGNDDLAA"
     gene            267..467
                     /locus_tag="KLK57_004615"
                     /pseudo
     CDS             267..467
                     /locus_tag="KLK57_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611683.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
BASE COUNT          174 a          128 c          142 g          122 t
ORIGIN      
        1 gactcgtatg acaacgcgat ggcggagagc atcaatggtc tttacaaagc ggaggtaata
       61 caccgtaaga gctggaaaaa ccgtgcagaa gtggaactgg ccatactcac gtgggtggac
      121 tggtataaca atcgacgatt gctggaaagg ctgggccata ctcctccggc agaagcagaa
      181 aaagcttatt atgcttccat cggaaacgat gatctggcag cctgagttca cagataaaac
      241 actctccagg aaacccgggg cggttcaacc ttcacgataa aaaacttttc attaaaggta
      301 aagcggatca atatccggca cttcattctc tgatatctcc gacccgcatt aatctgaaag
      361 aaatagaaat tcactggcgt gaagtgctac gtctggcgac ctcaataaaa cagggaacag
      421 tgacagcatc cctgatgctg agaaaacttg ccagttaccc caaacaaaag taaggcagta
      481 tgactttccg tgagacagat gctggccaac gtgggatatt taacgcgggt taacatcgtt
      541 gtcagagccg catgggtgag ctgttg
//
LOCUS       DAEEKQ010000472          558 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.484, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000472 DAEEKQ010000000
VERSION     DAEEKQ010000472.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.484"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>558
                     /gene="espX1"
                     /locus_tag="KLK57_004616"
     CDS             <1..>558
                     /gene="espX1"
                     /locus_tag="KLK57_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type III secretion system effector EspX1"
                     /protein_id="HBE0894757.1"
                     /translation="KHKGITLLRLDVFDDRTSECLSEEIKIYQECHEKFVNFLKANFN
                     QEIYPQLYTPEIFYEACRNLQSFYDHQETSQNAKYSAIDKKKSHLNQEIKKLIKKNIF
                     PELYNEQCNKIPSSSIDDNQGIMWQNFKTSNSAYSKLCGKLSLFKSVPSRLIEKSAYC
                     STENMATNKFNVVFSYCGDNIKEFIL"
BASE COUNT          221 a           98 c           85 g          154 t
ORIGIN      
        1 taaacacaaa ggtatcacat tacttagatt ggatgttttt gatgacagaa caagcgaatg
       61 tctttctgaa gagattaaga tataccaaga atgccatgaa aaattcgtta attttttaaa
      121 ggcaaacttc aaccaagaaa tatacccaca gctatatact cctgaaatat tttatgaggc
      181 ctgcagaaat ctccagtcat tttatgatca tcaggaaacg tcacaaaacg caaaatacag
      241 tgcaatagac aagaaaaaaa gtcatcttaa ccaggaaatc aaaaagctta ttaaaaaaaa
      301 tattttccct gagctataca atgagcaatg caataaaatc ccctcctcat cgatagatga
      361 taaccaggga ataatgtggc agaactttaa aactagcaat tcggcatatt caaaactatg
      421 tggaaaatta tctttattta aaagcgtgcc cagtcggtta atagaaaaat cagcttactg
      481 ctcaactgaa aacatggcca ctaataaatt taatgtcgtc ttttcttact gcggagataa
      541 tataaaggaa tttatact
//
LOCUS       DAEEKQ010000473          550 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.485, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000473 DAEEKQ010000000
VERSION     DAEEKQ010000473.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..550
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.485"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>550
                     /gene="feaR"
                     /locus_tag="KLK57_004617"
     CDS             <1..>550
                     /gene="feaR"
                     /locus_tag="KLK57_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HBE0894758.1"
                     /translation="QEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDASR
                     PCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMNNP
                     ALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEWIA
                     GETGMSVRSLYRMFADKGLVVA"
BASE COUNT          139 a          125 c          146 g          140 t
ORIGIN      
        1 ggcaggaagt aaaaggcagc gacgatgcct ggttttacac cgtttttcag cttagtggtc
       61 aggcaataat ggagcaggat gagcgtcagg tgcagattgg cgctggcgat attacgttac
      121 tcgatgcctc acgcccctgt tcgctttact ggcaggagtc ttctaaacag atttcattac
      181 ttttgccacg cactctgctg gaacaatatt ttccccatca aaaacctgtc tgcgcagaaa
      241 gactggacgc tgacttaccc atggtgcaac tcagtcatcg cctgttacag gagagcatga
      301 ataatccggc actttctgaa acagaaagtg aagctgcgct acaggcgatg gtgtgtctgc
      361 tgcgcccggt acttcatcag cgggaatctg ttcaacctcg ccgtgaacgt cagtttcaaa
      421 aagtggttac gttgatagac gataatattc gcgaagagat attacgcccg gagtggatag
      481 ccggagagac aggtatgtca gtacgtagtt tgtaccgaat gtttgccgat aaaggtttgg
      541 tagtcgcgca
//
LOCUS       DAEEKQ010000474          546 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.486, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000474 DAEEKQ010000000
VERSION     DAEEKQ010000474.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 546)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 546)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..546
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.486"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            1..546
                     /locus_tag="KLK57_004618"
                     /pseudo
     CDS             1..546
                     /locus_tag="KLK57_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT          194 a          120 c           83 g          149 t
ORIGIN      
        1 ccgacttatt aaaaatgcct gtttaaaatt atataattta gcacataccg caaccaactc
       61 ccaccttaaa ccaactggtc cggataattc agatgttctc ctgttcaaaa agctattctc
      121 cccttcgaaa ttaatgacaa ttatcggtga tgaaattcct cttatatctg aaaaacagtc
      181 gctttcaaag gtgcttttaa atgatgagaa taatgaactg agtgatggta caaacttctg
      241 ggataaaaat cgtcaattaa ccacagatga aatagcttgc tatcttcaga agatcgccgc
      301 taatgcaaaa aatactcaag tcaattatcc tactggtctc tacgtcccct actccaccag
      361 aactcacctg gaagacgctc tcaatgaaaa tattaagagc gatccatcat ggccgaatga
      421 agtccagtta ttccccataa atactggcgg acactggata ttagtttcgc tacagaaaat
      481 agtaaataaa aaaataataa actacaaata aaatgcgtca tattcaactc attgcgtgca
      541 ctaggc
//
LOCUS       DAEEKQ010000475          538 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.487, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000475 DAEEKQ010000000
VERSION     DAEEKQ010000475.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 538)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 538)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..538
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.487"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..508)
                     /locus_tag="KLK57_004619"
     CDS             complement(<1..508)
                     /locus_tag="KLK57_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001410998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HBE0894759.1"
                     /translation="MINHIDFSCDQSKDFSCDQSKIKIAETLFFNLDKEPYKNSRKLQ
                     ELIWDKLVAYVNDFSLSNQEKSRLILRLFDDVKLLFNEVPVSILVNDIFLKDFFMKQP
                     DFAKWYFYQLLKCYEGEQLYLNELGYVYGDEEKTKEIVNKLPGYVVKIFEEKMGNELK
                     IRTRMMKIL"
BASE COUNT          180 a          100 c           63 g          195 t
ORIGIN      
        1 ggaggatttt catcattctt gttctaattt ttaattcatt acccatcttt tcttcaaaaa
       61 tcttaacgac ataacccgga agtttattta caatttcttt tgttttttct tcatcaccat
      121 aaacatatcc taactcatta agatacagct gttcgccctc ataacactta agtaattgat
      181 agaagtacca cttagcaaaa tcaggttgct tcatgaaaaa atccttcaaa aaaatatcat
      241 ttactaaaat gctgacaggc acttcattaa atagtaactt aacatcatca aataacctta
      301 gtattaagcg tgatttttct tgattgctta agctaaaatc attgacatat gcaaccaact
      361 tatcccaaat caattcctgt aatttacggc tatttttata gggttcttta tcgagattga
      421 aaaataaggt ctcagctatt ttaattttag attgatcgca gctaaaatct ttagattgat
      481 cgcagctaaa atcaatatga tttatcacgg agcatgcata ctttatcttt tcagaatc
//
LOCUS       DAEEKQ010000476          520 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.489, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000476 DAEEKQ010000000
VERSION     DAEEKQ010000476.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..520
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.489"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>520
                     /locus_tag="KLK57_004620"
     CDS             <1..>520
                     /locus_tag="KLK57_004620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HBE0894760.1"
                     /translation="VMRTHFPEYEIISSASAEDLTLLQLRRSGLVIADLAGESEDPRS
                     VCEHYYSLISQYREIHWVFMVSRSWYSQAVELLMCPTATLLSDVEPIENLVKTVRSGN
                     THAERISAMLTSPAMNETHDFSYRSVILTLSERKVLRLLGKGWGINQIASLLKKSNKT
                     ISAQKNSAMRRLA"
BASE COUNT          138 a          121 c          117 g          144 t
ORIGIN      
        1 aggtcatgag gactcacttc cctgaatatg aaataatatc cagcgcctct gcggaggacc
       61 ttacattatt acaattacgt cgttccggat tagtcattgc tgatttagcc ggtgaaagtg
      121 aagatccacg ttctgtttgt gaacattatt attctttaat ctcacaatat cgggaaattc
      181 actgggtttt catggtgtca cgctcctggt attcccaggc agtagaattg ctcatgtgcc
      241 ctacggcgac gttattgtct gatgttgaac ccattgagaa tctggtcaag accgtacgtt
      301 ccggcaatac gcacgcagag cgtatcagcg ccatgctgac ctctccggca atgaatgaaa
      361 ctcatgattt tagctatcgc tccgtcattc tcactctttc agagcgcaag gtactgcggc
      421 tattaggtaa aggatggggc atcaaccaga tagcttcgtt gcttaagaaa agtaataaaa
      481 ctatcagcgc ccaaaaaaac agtgcgatgc gtcgactggc
//
LOCUS       DAEEKQ010000477          510 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.490, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000477 DAEEKQ010000000
VERSION     DAEEKQ010000477.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 510)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 510)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..510
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.490"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>510
                     /locus_tag="KLK57_004621"
     CDS             <1..>510
                     /locus_tag="KLK57_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HBE0894761.1"
                     /translation="TLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTSYQVAYNKYLV
                     QTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLSANIMQPLS
                     NNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENNKEEERFNL
                     FISIPFYWG"
BASE COUNT          157 a          100 c          105 g          148 t
ORIGIN      
        1 aactctggga aatggttgga atacaccgct gggtgccatc tctttttacg ctgcccgatc
       61 gagtagtaag ttaaataatg ataccagaca cgaaggtacg agctatcagg tcgcctataa
      121 taaatatcta gtacaaacag ccactcgatt tagcgtggcc gcctggcgtt atgcttcgca
      181 ggattacagg acattcagcg accatcttta cgaaaatgat aaacactatc atcagagtga
      241 ttatgatgat ttttatgata ttggcagaaa aaatagcctt tctgccaata ttatgcaacc
      301 tttatccaat aatctgggaa atgtatcatt aagtgcgctt tggcggaatt actgggggcg
      361 aagtggaaat gctaaagatt accaattcag ttactccaat aactggcaac acattagtta
      421 tactttctct gccagccaat cttatgatga aaataataaa gaagaggagc gttttaatct
      481 gtttatctcc attcctttct actgggggga
//
LOCUS       DAEEKQ010000478          507 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.491, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000478 DAEEKQ010000000
VERSION     DAEEKQ010000478.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.491"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..188)
                     /locus_tag="KLK57_004622"
     CDS             complement(<1..188)
                     /locus_tag="KLK57_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0894762.1"
                     /translation="MTKNTRFSPEVRQRAIRMVLESQDEYDSQWAAICSIAPKIGCTP
                     ETLRVRVRQHERDTGGGDG"
     gene            242..445
                     /locus_tag="KLK57_004623"
                     /pseudo
     CDS             242..445
                     /locus_tag="KLK57_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414790.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT          126 a          135 c          117 g          129 t
ORIGIN      
        1 ccatcaccgc ccccggtatc ccgctcatgc tggcgaaccc ggacacgcag agtctccggc
       61 gtacagccaa tctttggggc aatggaacaa attgccgccc actgtgagtc atattcatcc
      121 tgactttcca gaaccatacg aatcgcccgc tgacggactt cgggggaaaa acgagtattt
      181 ttagtcatcc tgtttacctc tttctcaggg agtttagtct ccaggatttc cggggcggtt
      241 cagacatcta gaatttactg tcagcaccgg cgttaaagtt tacttctgcg atcctcagag
      301 tccttggcag cggggaacaa atgagaatac aaatgggcta attcggcagt actttcctaa
      361 aaagacatgt cttgcccaat atactcaaca tcgagtaggc agcctgacgg ctgcggcttg
      421 tcatggtctg caattaccgt tataaaaaca ggcaatgtca ttgtctttca ggtggttata
      481 tggcccgttc agctaaaccc cgtaaac
//
LOCUS       DAEEKQ010000479          506 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.492, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000479 DAEEKQ010000000
VERSION     DAEEKQ010000479.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..506
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.492"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            224..>506
                     /locus_tag="KLK57_004624"
     CDS             224..>506
                     /locus_tag="KLK57_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0894763.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSL"
BASE COUNT          172 a           96 c          109 g          129 t
ORIGIN      
        1 gcttaaagat tttaacgatc gcttattgta aaaataatag caaagaaaat tgtaagatac
       61 agaccgtaaa taaaaacatc ttccttggtc aggaaagaaa tcttgaaagc atatctggct
      121 acgacaaaat cgttgtcaaa tataataact ggatcaccaa agataatggc gagtttgaac
      181 tggcggtcca ttaattatca ttgtaataaa cgaaggaata gtaatgaaaa aggtatttgc
      241 aaaatctctt ctggtcgcag cgatgttttc tgttgcaggc tccgcattgg cagtacaaaa
      301 ggatattact gtaacggcca acgttgatgc cgctctggat atgacacaga ccgataacac
      361 cgcgttgccg aaagcagtag aaatgcaata tctgccgggg cagggtctgc aatcttacca
      421 gttgatgacc aaaatctggt ctaacgacgt taccaaagat gtaaaaatgc agttagtttc
      481 cccggcgcaa ctggttcaaa gcctgg
//
LOCUS       DAEEKQ010000480          493 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.493, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000480 DAEEKQ010000000
VERSION     DAEEKQ010000480.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..493
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.493"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
BASE COUNT          173 a           96 c           81 g          143 t
ORIGIN      
        1 tttttatgcg gtttcaccgc accccgtgat gtcgccggga agccccggta aaaaataatt
       61 agcattagaa tagttgcgat aagctgcaat aagcagaacc acctttttgg tttaatatgt
      121 ccttacaaat agaaatgggt ctttacactt atctaagatt tttcctaaat cgacgcaact
      181 gtactcgtca ctacacgcac atacaacgga ggggggctgc gattttcaat aatgtgtgat
      241 gcagatcaca caaaacactc aattacttaa cataaatgta taagtcatac ttttgttttg
      301 ggtgtatttc aatatgttaa aaagtttttc gctacgctag caagcaaaaa tgaaacagga
      361 ataatcgaaa tgggatgttg cgcacagtta aaataaatca ccgtaaataa tcatctgcta
      421 taaataatca ctttcatgca ataccagata agctattttt aaacagacac ttaccacaca
      481 acaaactaat taa
//
LOCUS       DAEEKQ010000481          491 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.494, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000481 DAEEKQ010000000
VERSION     DAEEKQ010000481.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..491
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.494"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            30..206
                     /locus_tag="KLK57_004625"
     CDS             30..206
                     /locus_tag="KLK57_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894764.1"
                     /translation="MSFIFNEETLYFTVFGGDFIMDDLKIVKNTEAWVWYKNCDSKNK
                     KVAVELLQIYELHQ"
BASE COUNT          176 a           56 c          111 g          148 t
ORIGIN      
        1 aaggggtacg aataaagtaa cggggggcag tgagttttat ttttaatgag gaaacattgt
       61 attttactgt ttttggtggg gattttatta tggatgattt aaaaatagta aaaaatacgg
      121 aagcatgggt atggtataaa aattgcgata gtaaaaataa aaaagtggca gttgaacttc
      181 ttcaaatcta cgaattgcat caatgaaaaa tatagttaaa tagaggaggt tattaatagc
      241 aggaaaagca tcacgtgatg ctgatttttt gcactaaata attcgcgcta atgttcaata
      301 gttaatatat ccctcatcag ggaaagatca cggtgtgccg taggcacaaa gatgcaaaac
      361 gggacgtttt gcatgtaaca tgtaataagc ataagcttgt gaagagatgg tggcaggcca
      421 gggaaagatc atcagaagtt cactttttgt actaaataat tcgcatttta tgtttaaaaa
      481 ttgagatatt c
//
LOCUS       DAEEKQ010000482          489 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.495, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000482 DAEEKQ010000000
VERSION     DAEEKQ010000482.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.495"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..467
                     /locus_tag="KLK57_004626"
     CDS             <1..467
                     /locus_tag="KLK57_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HBE0894765.1"
                     /translation="ATTCNITLTGNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADF
                     KLVASGCSSGISWIDTTLTGNASSSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATF
                     LKPNSAEKIRWSTDEMQPDKGLEMTVALRETDAGQGVPGNFRALATFNFIYQ"
BASE COUNT          157 a          100 c          113 g          119 t
ORIGIN      
        1 aagcgacaac ctgtaacatc acacttactg gtaataacgt cacgaatgat ggcaataata
       61 actacacatt gagaatccct aagatgggtc tggataagat cgcgaataaa acgacagaat
      121 ctcaggctga ttttaaactg gttgctagtg ggtgcagcag tggcatcagt tggattgata
      181 ccactctgac cggaaatgca tcatcaagct cacctaagct tattataccg cagtctggtg
      241 attcatcttc gacgacaagt aatatcggta tgggtttcaa aaaacggact actgatgatg
      301 ccactttcct taaacctaac agtgcggaaa agatacgctg gagcacagac gagatgcagc
      361 ccgataaggg tcttgaaatg accgttgcgc tacgtgaaac agatgcaggg caaggcgtac
      421 cggggaattt tcgtgcactg gcaacgttta atttcatcta tcaataagaa ggataattaa
      481 gatgacatt
//
LOCUS       DAEEKQ010000483          489 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.496, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000483 DAEEKQ010000000
VERSION     DAEEKQ010000483.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 489)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 489)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..489
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.496"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="KLK57_004627"
     CDS             complement(<1..>489)
                     /gene="ipaH9.8"
                     /locus_tag="KLK57_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HBE0894766.1"
                     /translation="TGALLSLGREMFRLEILEDIARDKVRTLHFVDEIEVYLAFQTML
                     AEKLQLSTAVKEMRFYGVSGVTANDLRTAEAMVRSREENEFTDWFSLWGPWHAVLKRT
                     EADRWALAEEQKYEMLENEYPQRVADRLKASGLSGDADAEREAGAQVMRETEQQIYRQ
                     LTD"
BASE COUNT           99 a          153 c          117 g          120 t
ORIGIN      
        1 gtcagtcagc tgacggtaaa tctgctgttc agtctcacgc atcacctgtg caccggcttc
       61 cctctccgca tccgcatcac cgctcagacc tgatgctttc agccggtcag ccaccctctg
      121 agggtactca ttctccagca tctcatattt ctgctcttct gccagcgccc agcggtcagc
      181 ttccgtacgc ttcagtacag catgccatgg tccccagagg gagaaccagt ccgtaaattc
      241 attctcttca cggcttctga ccatggcttc ggcagtgcgg aggtcatttg ctgtcactcc
      301 cgacacgcca tagaaacgca tttccttcac ggcagtggag agctgaagtt tctctgcgag
      361 catggtctgg aaggccaggt agacttctat ctcatccaca aaatggagag ttctgacttt
      421 atcccgggca atgtcctcca gaatttcgag gcggaacatt tccctgccca gggagagcag
      481 agcgccggt
//
LOCUS       DAEEKQ010000484          483 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.497, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000484 DAEEKQ010000000
VERSION     DAEEKQ010000484.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..483
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.497"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..>483)
                     /locus_tag="KLK57_004628"
     CDS             complement(<1..>483)
                     /locus_tag="KLK57_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HBE0894767.1"
                     /translation="SRNGNITRWFYDSSRSGLPCAVEDGTGVRRRITRNRYGQLQAFT
                     DCSGYTTRYEYDRYGQQIAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGD
                     LTAIVAPDGSRSEIQYDAWGKAVSTTQGGLTRSMGYDAAGRITVLTNENGSQSTFRYD
                     "
BASE COUNT           82 a          158 c          134 g          109 t
ORIGIN      
        1 ggtcataccg gaacgtggac tggctgccgt tctcgttggt cagcacagtg atgcgcccgg
       61 cggcgtcata ccccatgctt cgcgtcagac cgccctgcgt ggtgctgacg gcctttcccc
      121 acgcatcata ctgtatctca ctgcggctgc cgtccggggc aacgatagcg gtgaggtctc
      181 ctgcggcgct gtactcataa cgggtttcac gcccctgcgc atccctctga ctgaccagtt
      241 gaccgcgcgg gttataactg ctgtaagtgc tgatgccttc ttcccggtga acggcgattt
      301 gttgaccgta ccggtcatac tcataccgcg tcgtgtaccc cgagcagtcc gtaaacgcct
      361 gaagctgacc gtaccggttt cgcgttattc tccgcctcac cccggttccg tcttccaccg
      421 cacagggcag gccactgcgg gaagaatcat aaaaccagcg cgtaatgtta ccgttgcgcg
      481 agg
//
LOCUS       DAEEKQ010000485          476 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.498, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000485 DAEEKQ010000000
VERSION     DAEEKQ010000485.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..476
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.498"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>476
                     /locus_tag="KLK57_004629"
     rRNA            <1..>476
                     /locus_tag="KLK57_004629"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          119 a          115 c          142 g          100 t
ORIGIN      
        1 tgtcgtcagc tcgtgttgtg aaatgttggg ttaagtcccg caacgagcgc aacccttatc
       61 ctttgttgcc agcggtccgg ccgggaactc aaaggagact gccagtgata aactggagga
      121 aggtggggat gacgtcaagt catcatggcc cttacgacca gggctacaca cgtgctacaa
      181 tggcgcatac aaagagaagc gacctcgcga gagcaagcgg acctcataaa gtgcgtcgta
      241 gtccggattg gagtctgcaa ctcgactcca tgaagtcgga atcgctagta atcgtggatc
      301 agaatgccac ggtgaatacg ttcccgggcc ttgtacacac cgcccgtcac accatgggag
      361 tgggttgcaa aagaagtagg tagcttaacc ttcgggaggg cgcttaccac tttgtgattc
      421 atgactgggg tgaagtcgta acaaggtaac cgtaggggaa cctgcggttg gatcac
//
LOCUS       DAEEKQ010000486          472 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.499, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000486 DAEEKQ010000000
VERSION     DAEEKQ010000486.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.499"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            89..451
                     /locus_tag="KLK57_004630"
     CDS             89..451
                     /locus_tag="KLK57_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001525284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HBE0894768.1"
                     /translation="MQTKQRLDVPLSLKSVSDSGEFEGYGSVFGVKDSHDDVVMSGAF
                     AASLRAWSDRKALPALLWQHRMDEPIGVYTEMKEDDVGLYVRGRLLIDDDPLAKRAHA
                     HMKAGSLTGLSIGYVLKD"
BASE COUNT          114 a          112 c          138 g          108 t
ORIGIN      
        1 cccggtggtg atgtctatct cacaccgatg aacatgacca cgaaaccctc cgatggcagt
       61 aaggccggta agcagaagga taacgccaat gcagacgaaa caacgtcttg atgtaccgct
      121 gagtctgaaa tctgtcagtg actccggtga gtttgaaggg tatggctccg tctttggtgt
      181 aaaggacagc cacgatgatg tggtgatgtc cggggcattt gctgcttccc tgcgggcgtg
      241 gagtgacaga aaagcgttac ctgcgctgct ctggcagcac cgcatggatg aacccatcgg
      301 tgtttacacc gaaatgaagg aagacgatgt cgggctttac gtcaggggac ggttgcttat
      361 tgatgatgat cccctcgcaa aacgtgcaca tgcacacatg aaggccggtt cgttaaccgg
      421 cctttctatt gggtacgtcc tgaaagactg aggtagcctg agtttaacgg ac
//
LOCUS       DAEEKQ010000487          472 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.500, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000487 DAEEKQ010000000
VERSION     DAEEKQ010000487.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.500"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
BASE COUNT           84 a          153 c          136 g           99 t
ORIGIN      
        1 ttgttgttct cctgcttatg ccttttccgg gcgtccccct ggcggggcgg gctgtcgtga
       61 acggagccag cgaagctgtc tccgccctgg cgggcttcca tccctgacgc aaaaacccgg
      121 gtttgcagcg accggaacgg tcgcgccacc cttgtcgcgc cgtggcctgc gctcccggca
      181 tcccggcaaa agacgccggg atgtaaggag caacgccgtt tcgcaaaccg cgcttcacgt
      241 cgttgcggcg ggtccctgcc cgttgttctg tccggtgcca ttcacctgtt taagcccctg
      301 tcgccggggg catagacagc cacaacctgc cgcactgact gtccagggta aatgaacagc
      361 ccacaaaatt ttttacccaa aacccggtaa aaaattttgc gttctgccca ggacaggtgc
      421 gtcaggccgt ggcagtgatg ccccttgcgg cgaaggttct caaacaggtg at
//
LOCUS       DAEEKQ010000488          471 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.denovo.501, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000488 DAEEKQ010000000
VERSION     DAEEKQ010000488.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.denovo.501"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..>471
                     /locus_tag="KLK57_004631"
     CDS             <1..>471
                     /locus_tag="KLK57_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001400151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HBE0894769.1"
                     /translation="YQLVSERAVKRADSRFELIQKNVGYFFKDIERSALTLKDSLYLL
                     KNTEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVYHQEQVNGPLVDE
                     SGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTLRINGKDHDL"
BASE COUNT          138 a           90 c          108 g          135 t
ORIGIN      
        1 tatcaactgg tatcggaacg cgcggtaaaa cgtgccgata gccgctttga acttattcag
       61 aaaaacgttg gctatttctt taaagatatt gaacgttcgg ccctgacatt aaaggactca
      121 ctgtatttat taaaaaatac agaggagatt caacgcgccg tgattctgaa aatggaaatg
      181 atgccatttt tagactcggt gggactggta cttgatgata ataaatatta tctcttttcg
      241 cggagggcga atgataaaat cgttgtttat catcaggaac aagtaaatgg accgcttgtc
      301 gacgagtcag ggcgggttat ttttgccgat tttaacccat cgaaacgacc gtggtcggtg
      361 gcttcagatg actctaacaa cagctggaat ccggcataca attgctttga tcgtccgggt
      421 aaaaaatgta tctcttttac gctacgtatc aacggcaaag atcacgattt g
//
LOCUS       DAEEKQ010000489        12010 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.guided.116, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000489 DAEEKQ010000000
VERSION     DAEEKQ010000489.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12010
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.guided.116"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..302
                     /locus_tag="KLK57_004632"
     CDS             <1..302
                     /locus_tag="KLK57_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HBE0894770.1"
                     /translation="YAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            397..921
                     /gene="sat2"
                     /locus_tag="KLK57_004633"
     CDS             397..921
                     /gene="sat2"
                     /locus_tag="KLK57_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HBE0894771.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            979..1767
                     /gene="aadA1"
                     /locus_tag="KLK57_004634"
     CDS             979..1767
                     /gene="aadA1"
                     /locus_tag="KLK57_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HBE0894772.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            1843..2340
                     /locus_tag="KLK57_004635"
     CDS             1843..2340
                     /locus_tag="KLK57_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894773.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            2401..2772
                     /locus_tag="KLK57_004636"
     CDS             2401..2772
                     /locus_tag="KLK57_004636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894774.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            3182..3559
                     /locus_tag="KLK57_004637"
     CDS             3182..3559
                     /locus_tag="KLK57_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894775.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            3582..3734
                     /locus_tag="KLK57_004638"
                     /pseudo
     CDS             3582..3734
                     /locus_tag="KLK57_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3790..5406)
                     /locus_tag="KLK57_004639"
     CDS             complement(3790..5406)
                     /locus_tag="KLK57_004639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HBE0894776.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALEQSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(5407..6933)
                     /locus_tag="KLK57_004640"
     CDS             complement(5407..6933)
                     /locus_tag="KLK57_004640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HBE0894777.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            complement(6936..8603)
                     /locus_tag="KLK57_004641"
     CDS             complement(6936..8603)
                     /locus_tag="KLK57_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HBE0894778.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            complement(8600..10708)
                     /locus_tag="KLK57_004642"
     CDS             complement(8600..10708)
                     /locus_tag="KLK57_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HBE0894779.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            complement(10695..11516)
                     /locus_tag="KLK57_004643"
     CDS             complement(10695..11516)
                     /locus_tag="KLK57_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HBE0894780.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            complement(11675..>12010)
                     /locus_tag="KLK57_004644"
     CDS             complement(11675..>12010)
                     /locus_tag="KLK57_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HBE0894781.1"
                     /translation="AAGELKHGPLALIDADMPVIVVAPNNELLEKLKSNIEEVRARGG
                     QLYVFADQDAGFVSSDNMHIIEMPHVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPR
                     NLAKSVTVE"
BASE COUNT         3268 a         2935 c         2508 g         3299 t
ORIGIN      
        1 agtatgcggt cgtaacacgt tcaagtttta catctgacaa tgagaacgta ttgatctttc
       61 catcaattaa agatgcttta accaacctaa agaaaataac ggatcatgtc attgtttcag
      121 gtggtgggga gatatacaaa agcctgatcg atcaagtaga tacactacat atatctacaa
      181 tagacatcga gccggaaggt gatgtttact ttcctgaaat ccccagcaat tttaggccag
      241 tttttaccca agacttcgcc tctaacataa attatagtta ccaaatctgg caaaagggtt
      301 aacaagtggc agcaacggat tcgcaaacct gtcacgcctt ttgtaccaaa agccgcgcca
      361 ggtttgcgat ccgctgtgcc aggcgttagg cgtcatatga agatttcggt gatccctgag
      421 caggtggcgg aaacattgga tgctgagaac catttcattg ttcgtgaagt gttcgatgtg
      481 cacctatccg accaaggctt tgaactatct accagaagtg tgagccccta ccggaaggat
      541 tacatctcgg atgatgactc tgatgaagac tctgcttgct atggcgcatt catcgaccaa
      601 gagcttgtcg ggaagattga actcaactca acatggaacg atctagcctc tatcgaacac
      661 attgttgtgt cgcacacgca ccgaggcaaa ggagtcgcgc acagtctcat cgaatttgcg
      721 aaaaagtggg cactaagcag acagctcctt ggcatacgat tagagacaca aacgaacaat
      781 gtacctgcct gcaatttgta cgcaaaatgt ggctttactc tcggcggcat tgacctgttc
      841 acgtataaaa ctagacctca agtctcgaac gaaacagcga tgtactggta ctggttctcg
      901 ggagcacagg atgacgccta acaattcatt caagccgaca ccgcttcgcg gcgcggctta
      961 attcaggagt taaacatcat gagggaagcg gtgatcgccg aagtatcgac tcaactatca
     1021 gaggtagttg gcgtcatcga gcgccatctc gaaccgacgt tgctggccgt acatttgtac
     1081 ggctccgcag tggatggcgg cctgaagcca cacagtgata ttgatttgct ggttacggtg
     1141 accgtaaggc ttgatgaaac aacgcggcga gctttgatca acgacctttt ggaaacttcg
     1201 gcttcccctg gagagagcga gattctccgc gctgtagaag tcaccattgt tgtgcacgac
     1261 gacatcattc cgtggcgtta tccagctaag cgcgaactgc aatttggaga atggcagcgc
     1321 aatgacattc ttgcaggtat cttcgagcca gccacgatcg acattgatct ggctatcttg
     1381 ctgacaaaag caagagaaca tagcgttgcc ttggtaggtc cagcggcgga ggaactcttt
     1441 gatccggttc ctgaacagga tctatttgag gcgctaaatg aaaccttaac gctatggaac
     1501 tcgccgcccg actgggctgg cgatgagcga aatgtagtgc ttacgttgtc ccgcatttgg
     1561 tacagcgcag taaccggcaa aatcgcgccg aaggatgtcg ctgccgactg ggcaatggag
     1621 cgcctgccgg cccagtatca gcccgtcata cttgaagcta ggcaggctta tcttggacaa
     1681 gaagatcgct tggcctcgcg cgcagatcag ttggaagaat ttgttcacta cgtgaaaggc
     1741 gagatcacca aggtagtcgg caaataatgt ctaacaattc gttcaagccg acgccgcttc
     1801 gcggcgcggc ttaactcaag cgttagagag ctggggaaga ctatgcgcga tctgttgaag
     1861 gtggttctaa gcctcgtact tgcgatggca tcggggcagg cacttgctga cctgccaatt
     1921 gttttagtgg atgaagctcg tcttccctat gactactccc catccaacta cgacatttct
     1981 ccaagcaact acgacaactc cataagcaat tacgacaata gtccatcaaa ttacgacaac
     2041 tctgagagca actacgataa tagttcatcc aattacgaca atagtcgcaa cggaaatcgt
     2101 aggcttatat atagcgcaaa tgggtctcgc actttcgccg gctactacgt cattgccaac
     2161 aatgggacaa cgaacttctt ttccacatct ggcaaaagga tgttctacac cccaaaaggg
     2221 gggcgcggcg tctatggcgg caaagatggg agcttctgcg gggcattggt cgtcataaat
     2281 ggccaatttt cgcttgccct gacagataac ggcctgaaga tcatgtatct aagcaactag
     2341 cctgctctct aataaaatgt taggcctcaa catctagtcg caagctgagg ggaaccacta
     2401 gtgtcatacg aacctccaag agacggttac acaaacgggt acattgttga tgtcatgtat
     2461 gacaatcgcc caagtaagta tccagctgtg ttcagaacgt acgtccgaat tccagactat
     2521 gtcgaactag ataacgcgga ggagctggcc gacgcaatca ttcgagaggt tgagttaaag
     2581 gaggcatggc tcacaagggt cttggggaac tcagccgatc ctgacgcttc tgtgggcaag
     2641 tgggaccggc gcctagcgca gtgcggtggt attgatgaga tgtcaagtgg ggtgcaggca
     2701 acatacttca gtccaaacgg tcagctggtc ggcttagtgg catacattga tgccgaatgg
     2761 atggatgaat agctctgacg ttacgattca ggtgagacaa ccagatgggc attgcgtaca
     2821 agctcagttg ccaccgatgt ggctacgagt ctgatcttct ttacctaggc caaggtatgg
     2881 cgatgcttcc tgagcaaata gtcggaacgt gcacatgctg cgacaatcta acgacgattt
     2941 ctataaatga aagctacgga gtatgctcac tttgtggggc taaggatcgt gtgatattta
     3001 caagctatca gcatgaggca aggccaagaa atccctgcta tctagagaga agtttcaaat
     3061 atcaatgccc aagatgccat gccttcagta tggagcctcc ggatctaccg gagatgcttt
     3121 gggactaatg ctccgctttg cctcgggtat ccgttcgacc tatgtttaag aggaaacgca
     3181 cgtgtctgcc gccaaagctc ttgcctatgc actttgtgac aagttggaaa gcaaaaaagt
     3241 tgatgaagtc gtacgaaatc ttgtttctag cggctggaat attagggaag tggcctggga
     3301 agcttcgtcc tttgatcatg aaatgcccta ctttgacacg gggcgggact tcgatattgg
     3361 atttcctgac acgctgtcat ccaagattga gtttttgacc gcgaatggat caatcgtcca
     3421 agcacgtgct cgcctgcact tcaagcaggc tctaattttt gccaaggcgt ccaaacatgt
     3481 cgccgcgctc aagaactcat tggattacta ctatggatcg tctgtaatcc cccctcaaaa
     3541 ccacggaggc tatttgtaga aaattccatt aatatgaact caaggtgttc cctacggacg
     3601 aatcagcgat gaaggtggtg tacctggcta tccaggcggc gaccaagaaa tggacgatgc
     3661 cgatccgcaa ctggaagccg gccatgaacc gttttatgat cgaatttggt gaccgtttaa
     3721 atggccacct ttaactgacc ggcaaaagca cttacacaaa agggtttaca ggctctttca
     3781 gtcattcctt taatgcgtaa attgctctct aaccactctc tcagcccacc cttccatatc
     3841 ctctctcgat acttgcagct cccccaaccc ctttggatga ttcacccctc tatgcccgtc
     3901 ttgtccatat aattgatcaa acaaactatt tggccaattc aatgagctct tcaccactcc
     3961 acgccgtatc ttttcaaaat cattccgcca tgtttcgcta tcaacgccac tcaacacttt
     4021 ggtagaaagc attttcaccc catccgatgc atccacctct agcaatacaa attcactgcc
     4081 attacgttgc accccaacgg ctttaatcac tcttggtgag ccatctttac acagatgtaa
     4141 tcggctacgc ccaacttttg gcaaaaccaa ggtttcttca aaaagcaccc gacatgcaaa
     4201 tttagttttt agaattgaaa gtagctcatc aaaagcagca aatcgattag caaaaataga
     4261 gttgtaattg gttgcatcct gcttcccacc cacatctgcc gctgctagga caccacctaa
     4321 gtgtggctca tcagtactaa ccaaattact attttcactt gttgttgctt cttctttttc
     4381 taaaatagtt tggctacttt ttcttgccct gcgagatttt tcaattcgac taggtctact
     4441 aaaacttatc catgtcggct cagcctctat gactcgtgtg tctgtgtcta tattggctgt
     4501 ttcctcatcg tccaactgta gatgctcatc atcaacaact gactctgttg aaaccgctat
     4561 gtgctgcgta ctaccatcac ctgcctcctt ttcctgaaaa gaggcatggc taatagctgt
     4621 tgtgctagga agcatagcgt tgatttctaa acctactatt tcctcaacta aataatcctt
     4681 atcctcgtta gaagaacgtc cttttacatg taatttccaa ccctgcatgg gcggagggtc
     4741 aaagctaaag caccagcttt caacgccgtt cttaatttct ctattttgtt gatagtgcct
     4801 aaaaatactt tcatacgaat ccataacatc ttgatccgaa aacaaccaaa ccaaaagctg
     4861 cactacggcc gactgctcta acgccccttt aggaaacgat gaactgggta agatccttat
     4921 ctctaaatga tctcgctcaa cctcatactg aacgtcgaac tcttgctgta aagcggtact
     4981 gctcaaacag cttcgacaga aataggagtt aattaaaaat aatgaccgag ctagctctaa
     5041 ctgtgggatg tgataacgag tcttgctata tggtgtttca gcttcgtaaa taaatgaact
     5101 ctgcgctcca tccttgttgc gtacggaagc aaaggacgag agattaggaa aatcgattat
     5161 tttggctcgt tgaagatccg atgcttcaaa ctcaatcaca cgatccgcct tatttatttg
     5221 ctttgttgta ctgttaacca ccttagctcg acttaataaa ggcaaatgag taaatcgagt
     5281 ccatttccgt tcttgcattg gattaaacca aacaaaaata cgccactcct tatgacccga
     5341 gttacggaat aaatgaccaa tatgtacaac ctgcacattg tcattaaatg tagctagcct
     5401 aaccactcac tcttccccat aaaccatttc caagaatctt tgtgcttcct cagttatccg
     5461 ctctttagat aacgttgcac ttcttaataa tcgccagcgc ctaagctcaa catcttcctg
     5521 tttaagctta ataaaagctt ggctaatccg tctaatttga taatcttcca cactctctga
     5581 gtaacgcttt aagcaaagcg ctaccaaagg cagtttctgt agattttttg caagagaggt
     5641 tccgttagga gtttgcttta acagccagct cgatgtcgct cttgggtgat caaggctact
     5701 atctagacgc tttatgattc ttaatagttg tcgtacagca attcgatctc tttggttcca
     5761 atcaactcta ggggcgggtg ccagacgctc ttgttgatgc tgttgattcc agtgaactag
     5821 ccaatccctg tcatgtcggt aaagccaagc gtatagcacc ccaccctcta aagactgtct
     5881 tgccgcctta attccttggt atttatgcac tagttgctgc cagtctttat gcttaacaga
     5941 taaatcttca gagttaggtt gcacagactg gctaacaggt cttgtcgtta tagagtgctc
     6001 agtaagagca cttgcctgct gtagcgcttc tataaccgtt agttttggca ataaggcttg
     6061 ccacacaata ctatgctgta aataactaaa ggcttttcta tgcttacgga atatactttt
     6121 cagccaacac gtgtccttgt tctctgccaa ctttaaatcc agtttctcta gtgcctcatc
     6181 actaaaagtc tgcctcactc tctccgccac caagtcatga cgaatgtgct tgcttttggt
     6241 tagccctaga tcctgcgcta agcgctgata aaacagcgtc cactgctcaa ggcttgggga
     6301 aagctcttgc gctcgtggag catctaacag aggggctata taagcagcta gtgctgttaa
     6361 ttgagatagg gagtctttgg ggtagtctga aagcagctca gtatgaccca aagcccaaaa
     6421 ttgatgtcgg tgatcatcta cagctctatc aaagaagact aaagcaccgt gttttggaca
     6481 atatggcaaa gcgggcaaat accaatctcg ttgccaaaag gcttccccat acctatttag
     6541 ctgaagagca acgcaatcag ggcagtatct aaagcggtta tcgctcttaa ctctagaagc
     6601 agcgactcct agcattaaat gcaccgcacc ttgcgcttgg tactccatta accgaatagc
     6661 ttcgtctcgg cgctccttgc ctacaaacgg agcatataaa gggaataagg tatgctcata
     6721 aataagctgc tgaacagcgt aacgtcctgt ttgatgtaga tgtcttgcta tcacacctaa
     6781 atgcgagggc agacctaagg tagcgaccac cttgcggttg ccatacacct catccaacag
     6841 ctgcttagga ctaacaatcc cttgataaac gcctgcccgt gcaatagtgc tataaatcag
     6901 ctcattcgag tacggaacag gaaagtttct catggctaac ctgcttgttt aaataagctc
     6961 gccatatcca ctattacccc tttccctttt aaccgttcat gcatggtttt ttcaggttgg
     7021 cgttgtgaat agatataacg taaatccgta tcaggcaagc tatcccagtc gctgggcttg
     7081 actaccttta tagccgaaac ctttttactc ttggagggct tttctagttc tgacactacc
     7141 gtttcgcctt ccatcaacca ctgcaaaaca agaggcagta acttttgtct tgtcatcgtt
     7201 ggattctggc taaacgcttt tttaatagtg ggaattaaca ggcttgaatc gtaatcctct
     7261 ttcagcatca gatataaatg acgctgatct tcggtatcta actcttgaag ggctttttct
     7321 tctggtgttt gttcttgtat cgctgcgata tctagctgaa gttggattaa ccgtttatca
     7381 atctcgggaa cgactagatc agaataacga gcaatgcgtt ctgggatacc cgagcgtaat
     7441 gcctctagca tggggtgcac aggctttaac tcatcttgat acacttgccg caataaacca
     7501 gcggtaatac gctcattgcc taaagctagc gcacggagct gagcgagtac aaaaagtttt
     7561 actacaatgt ccatcactcc ttggcttagc tcataccaca catcacggac ctcatccgat
     7621 aacagcgcat ctttgcgttg taaaagctgt aattgccaaa gattatccgt aaaagcgatc
     7681 cactcttgat tgggctttcc acgttgcgtt tgttgtatag gatcccagaa tatagctcca
     7741 aaccctgccc ctctacgtgc agaccgcaaa tcagcctcaa aaatctctcg tgctttaggg
     7801 gtaccaatca acatcactgg tacgccaata atattcacca tcgtcacaaa aaagttcagc
     7861 atctcttgag atccacccga acgagagcgg cttaaatgct gaatttcatc aataaccaac
     7921 aaccctaaag catgtgcatt ggctatttgc gacatcaaag ccaacatggt ttctatacca
     7981 tgacgtttta agccataacg acgctcatag ttcgagccca aggctcgatc caacgctctg
     8041 aaaaaattca agcagatttc ttttagcgaa ccattatgcg agcagtctat tttcaaatac
     8101 accacctgct ctacattgag ttcacgatgg taaatcacct gaggatacgt ggctagaata
     8161 cgatgaagag aggtcgtctt cccactacca gaacaaccaa ttaacaataa gctttgtgcc
     8221 gtagatcgtg cctcctcaaa gcgaaatgtc tccaactctc ccgtttgaac acgctcataa
     8281 ccattttgta aatgcttttg taaatctcct gttttaggat ttctgcctac gtagccacct
     8341 cgaatcatga ccgaaatacg ctcacttagt agcaaatgcg tacctaatgg ctgaaaatag
     8401 tcatctggaa tacgacaaat ggtatgagct ctgataacac gggacttttg caagtcagag
     8461 gaagtaagct gtaaagagga tttcagtgat gcagcactat tcactgactc ttgtaatggt
     8521 ggtaaggcct cgataaaagg attatcacga taagcctcta cccccgtatc acgataaact
     8581 gcttgaatcc gggtagcact catgactcat ccttttctgg tggatcctga aataattcag
     8641 gcacgtatgt gggtaggctg taatcttctt gatcatccgc ttccactgcg ttgaaaggaa
     8701 taactttagc ctcatcacct gaagagcttg gcttcaaatg ctccgcacgt tttttacgct
     8761 ccgaggtcac ggcttctttt ttattagttt taatctgctt aatgcgtgtt gatttgggct
     8821 cagtagtact gggcgttaac ttattcgctt tctgaatggt ttgctgaata aacgcctcaa
     8881 gctccctgcg tttagttaac tcatcctgct tcgcattggc tttattgtgt ttttcttgtg
     8941 cttgtatatc ccaaacctcc caaaatgaga gacctttaaa ctgccgacta cgttccgtca
     9001 gattacagcg ccaaaataca cggctgccaa cttgcggaaa caaataaatc gtatcaacca
     9061 gcactgggtc ataagccgct tctaaatgtt gaggtctagc tatatcagtg ctccgctgca
     9121 accaaccctc acgcagaatc tctgaccccg agtaatacaa accccacaaa ttaacgccaa
     9181 atgaagaaat agagaccttt cggcgaggca gtaacgctac tcgcaactgc tcttgctcca
     9241 cagcccttaa actacctgta cgatgctgca taccccattg ccatagctgg acaggaatag
     9301 acggtaaatc tgtaggaaaa tcagcatctc gatcgtattt atccatcacc agatggttat
     9361 ttctgaataa gatcgtacgc aaaataattt gtgtgaactc aaataccgac agagatgcat
     9421 ctaacctata gtctgtttca ccatggcttt tgatccgact gccctctaca atgccaggtg
     9481 caaaggactt aaactcggct tgtagtgttc taaaagtgct ttccactatg cctttagcat
     9541 cgccacgtct aggtggagca ctttccactc gcacattaaa actagaaact aaggcttcga
     9601 cctgatgact cattaattcg ccacggtccg ctagcaacac atctggcaaa cctacacacg
     9661 gccagtctga gctactaatc tcaatatcat gctgggcaca aatggccgtt ttgtcagagc
     9721 aagcatttac aaaagcctgc atcgccacca cataagacgg attttcaaag ccgatataaa
     9781 agcccgtgat catccgacta aacacatcaa tcacaatgta aagcgttggt cttcctatga
     9841 ttttttggcg atcatgatga tccactaaat aaatatcagc aatcgtggca tcaatctcat
     9901 aacgactccc agggcctaac gcctgagaag tggctgtact acttaagggt cgtacgtctt
     9961 ttttatatac ccctgcttta actctagact ttaagcgctg agctttaggg tattctcgat
    10021 cataaaaata acgaaactga cgtagtgttg ggtaatcctc ttggggaatg cgaggaaaat
    10081 actgagcaaa caagtccaca aatcgtctat aggcaacggt ggtctttgta cctttttgat
    10141 ttaacaggtg cttttctatg gtcaacctaa aaaggcgttc aatctcgggc gttaccttgg
    10201 ttccttcacc cttaccatat tctctggctc ggccaatctt tgctgttcct gtcgctgaac
    10261 gtctttcccc tggtgcaccg ctgtttttgt agtcaggaat taatgcatta ggcgtttgac
    10321 cacgctgcca gtaacggcgt aacaacttat aaactgtagc cttagtaacc ttatgttctt
    10381 ggaccacatg ctcaacgagt tcgcttctga ctttagggtc gaaacgatcc ttactattaa
    10441 taataggaag aatttttcga taatcctcct cgcgcttctg gaagctgaca gaatctacag
    10501 acggctcttc taaatcaaga tgtacataag ggtcatcagt tctaactaag cggccttcat
    10561 ctaaatactg cattagcaac aactcagccc tagcttgtgg aactccttta tcagcgctta
    10621 tttgcatcca gacaacttgg ccatcctcta tagccaaaat gcgatacgga tcattatcaa
    10681 atagcacaac ctcattaatt tgccacatag cgcaactcct ccatattcac tacttggcta
    10741 atacagagat ctgcacactt atttgccctg aaagacttat aaatattgaa cttaataaaa
    10801 ccatttgcgg ttaaggctcg tatctcactc aatgttttgc ctaactccaa atcataagca
    10861 atatcaacct gcttacagac attgataatg ttttcatctc ctttttcttg caggatatgg
    10921 gccaatgggg atagttgtgc taaaagctcc gcagaaactt cttctgtttt cactgaataa
    10981 agccattcaa tattttcttt tactacggga tttatttctt tatcagtaaa aatgaaccaa
    11041 ggaatttgct tttgctgcca atagcgacgc tctagttcta gtttttctaa ggtacgctcg
    11101 tcttgtaagg ctgctgcagg tttgacttga atagcaaact gctcaaaagg accatctttg
    11161 cagtccacta aaaaatcagt agacataacc tgatctacac cacgaataac aggatgctta
    11221 ataccactat ctattgcaat ctgcctggta tcactaggta ataaggggaa ctgctcgcgt
    11281 atatctagca cgctgctctc ccactcaaga ctgagaaaaa cagcaagctc taagtcagat
    11341 agcaaatgat ggactcgtcc cgtcttatga gaataaatac ggtgggaacg acctgaagaa
    11401 ggaacttctt gtactgttag ccatggaata tagtctttac catgcccttg gccacgcccc
    11461 tctttaatac gacgggcaat ttgcacttca gaaaatgaag agtttgcttt agccataaca
    11521 aaagtccagt atgctttttc acagcataac tggactgatt tcagtttaca actattctgt
    11581 ctagtttaag actttattgt catagtttag atctattttg ttcagtttaa gactttattg
    11641 tccgcccaca gccctttttt atctatgata gtgattactc aaccgtaacc gattttgcca
    11701 ggttacgcgg ctggtcaacg tcggtgcctt tgatcagcgc gacatggtaa gccagcagct
    11761 gcagcggaac ggtgtagaag atcggtgcaa tcacctcttc cacatgcggc atctcgatga
    11821 tgtgcatgtt atcgctactt acaaaacccg catcctgatc ggcgaagaca tacaattggc
    11881 cgccacgcgc gcgaacttct tcaatgttgg atttcagttt ttccagcaat tcgttgttcg
    11941 gtgcaacgac gataaccggc atatcggcat caataagcgc cagcggaccg tgtttcagtt
    12001 caccagcagc
//
LOCUS       DAEEKQ010000490        10228 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.guided.140, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000490 DAEEKQ010000000
VERSION     DAEEKQ010000490.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10228
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.guided.140"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            836..1915
                     /locus_tag="KLK57_004645"
     CDS             836..1915
                     /locus_tag="KLK57_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HBE0894782.1"
                     /translation="MLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYTDR
                     IAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTART
                     VKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFTVF
                     QGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHV
                     SSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANELW
                     KKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPDLT
                     ASPPHKKNVAQTPSGGKQESRKKRF"
     gene            2779..3594
                     /gene="sul2"
                     /locus_tag="KLK57_004646"
     CDS             2779..3594
                     /gene="sul2"
                     /locus_tag="KLK57_004646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBE0894783.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            3655..4458
                     /locus_tag="KLK57_004647"
                     /note="aph(3'')-Ib"
     CDS             3655..4458
                     /locus_tag="KLK57_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBE0894784.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            4458..5294
                     /locus_tag="KLK57_004648"
     CDS             4458..5294
                     /locus_tag="KLK57_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HBE0894785.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5266..5406)
                     /locus_tag="KLK57_004649"
                     /pseudo
     CDS             complement(5266..5406)
                     /locus_tag="KLK57_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000845049.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(5401..5670)
                     /locus_tag="KLK57_004650"
                     /pseudo
     CDS             complement(5401..5670)
                     /locus_tag="KLK57_004650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
     gene            complement(5900..6331)
                     /locus_tag="KLK57_004651"
     CDS             complement(5900..6331)
                     /locus_tag="KLK57_004651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HBE0894786.1"
                     /translation="MVLRSIWIEEQYMGVKKIVMLAILVSFVAGCAPLHPSNCHKTTA
                     LGSCSSGRWDDQDEWGEQARGIRDAINAKLDDPQKWKRKKCRLHMEFSRDGTALKIST
                     SNGDKAYCEAIKSAAHKAKFPAFNNPEVYRDFQKSGFDMRG"
     gene            complement(6399..9110)
                     /locus_tag="KLK57_004652"
     CDS             complement(6399..9110)
                     /locus_tag="KLK57_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HBE0894787.1"
                     /translation="MNAIIPHKRRDGRSSFEDLIAYTSVRDDVQENELTEDSEIKSDV
                     PHRNRFRRLVDYATRLRNEKFVSLIDVMKDGSQWVNFYGVTCFHNCNSLETAAEEMQY
                     KADKAVFSRNDTDPVFHYILSWPAHESPRPEQLFDCVRHTLKSLELSKHQYVAAVHTD
                     TDNLHVHVAVNRVHPETGYINWLSWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRI
                     VRKTALVRERRNAWRRGKKQTFREYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITM
                     QEGELVVKDGWDRAREGVALSSFGPSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDP
                     EAINVDIRPEKVAETESLKQYACRHFAERLPEMARNGELESCLDVHRTLAKAGLWMGI
                     QHGHLVLHDGFDKQQTPVRADSVWPLMTLDYMQDLDGGWQPVPKDIFTQVIPGERFRG
                     RNLGTQAVSDYEWYRIRMGTGPQGAIKRELFSDKESLWGYTTVQCESLIEDMIAGGNF
                     SWQACHEMFARKGLMLQKQHHGLVIVDAFNHELTPVKASSIHPDLTLSRAEPQAGPFE
                     IAGADIFERVKPECRYNPELAASDEVELGFRRDPVLRRERREARAAAREDLRARYLAW
                     KEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDPQLRKLHYHIAEVQRMQALIRLK
                     ESVKEERLSLIAEGKWYPLSYRQWVEQQAVQGDRAALSQLRGWDYRDRRKDKRRTTNA
                     DRCVILCEPGGTPLYEDTGVLEARLQKDGSVRFRDRRNGELVCVDYGDRVVFYHHQDR
                     NELVDKLNLIAPVLFDREPRMGFEPEGSYQQFNDVFAEMVAWHNAAGITGSGHFVISR
                     PDVDLHRQRSEQYYHEYIRQQKSISGGHGASYAPVQDNEWTPPSPGM"
     gene            complement(9122..9454)
                     /locus_tag="KLK57_004653"
     CDS             complement(9122..9454)
                     /locus_tag="KLK57_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nikA protein"
                     /protein_id="HBE0894788.1"
                     /translation="MSEKKTRTGSENRKRIVKFTARFTEDEAEIVREKAESSGQTVST
                     FIRSSSLDKVVNCRTDDRMIDEIMRLGRLQKHLFVEGKRTGDKEYAEVLVAITEYVNA
                     LRRDLMGR"
     gene            9687..10022
                     /locus_tag="KLK57_004654"
     CDS             9687..10022
                     /locus_tag="KLK57_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein MobC"
                     /protein_id="HBE0894789.1"
                     /translation="MASKKFYSDDDIQLAKAALSELPDLTAQRKTLRDFLDAIRDDII
                     ILVRTKGYTLTDVRDTLKNAGYEAGEKALRDIIREAESKKPSRRSSSKTAPKKTDSGR
                     KDGIDMNNN"
     gene            complement(10108..>10228)
                     /locus_tag="KLK57_004655"
     CDS             complement(10108..>10228)
                     /locus_tag="KLK57_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001401764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HBE0894790.1"
                     /translation="TVRGYQKGTVHIVFKRSDLVEKLNDIIARHYPGALPPRV"
BASE COUNT         2506 a         2804 c         2444 g         2474 t
ORIGIN      
        1 gagcatggat gacctcaccc ggcatgtcat gttttttgcc accaccggtg gcggtaaaac
       61 agaaaccacc tttgcctggc tgctcaaccc gctctgctgg ggaagaggct ttacctttgt
      121 tgacggaaag gcacagaacg ataccacgag gacaatctgg tatctctccc gacgttttgg
      181 cagggaggat gatatcgaag tcatcaactt catgaacggc ggaaaatccc gcagtgaaat
      241 cattcagagc ggcgaaaaat cccgcccaca gtcaaacacc tggaacccgt tcgcattcag
      301 caccgaagcc ttcaccgccg aaaccatgca gtccatgctg ccccagaacg ttcagggcgg
      361 tgaatggcag tcacgggcga tagccatgaa caaggccctg gtctttggca caaaattctg
      421 gtgcgtccgg gaaggtaaaa ccatgtccct gcagatgtta cgggaacata tgacgctgga
      481 agggatggca aaactctatt gccgtggcct tgatgaccag tggccggaag aagctatcgc
      541 accactgcga aactacctgc aggatgtccc gggattcgat atgtcactgg tgagaacgcc
      601 gtcagcctgg actgaggagc cccgtaaaca gcatgcttat ctctccggtc agttctcgga
      661 aacgttcaca acctttgccg aaaccttcgg ggacgtcttt gcggcagatg ccggcgacat
      721 cgatattcgc gacagtattc acagcgaccg cattctgatt gtgatgatcc ctgcactgga
      781 cacatcggct cataccacgt cggcactcgg tcgtatgttc gtcacccagc agagcatgct
      841 cctcgcccgt gacctgggct accggctgga aggcacagat gcacagaccc tggaagtcaa
      901 aaaatacaaa ggcagcttcc cctatatctg tatccttgac gaagtcggtg cctactacac
      961 agaccgcatt gctgtggagg ccacccaggt acgttcactg gagttttccc tgataatgac
     1021 ggcgcaggat caggagcgaa ttgaagggca gacctccgcc accaacaccg ccacactgat
     1081 gcagaacgcc ggcaccaaat tcgccggacg cattgtcagt gacgacaaaa cggcaagaac
     1141 ggtgaaaaat gccgccggtg aagaggcacg ggccagaatg ggcagcctgc agcgccacga
     1201 tggtgtgatg ggtgagtcct gggtggacgg taaccagatc accatccaga tggaaagcaa
     1261 aatcgacgtg caggatttga tcagactgaa tgctggtgag ttctttaccg ttttccaggg
     1321 ggatgtggtg ccgtcagcct ctgtgtatat tccggacagc gagaaaagtt gcgacagtga
     1381 tccggtggtc atcaaccgct atatttccgt tgaagccccc cggctggagc gtctccgccg
     1441 tctcgtcccc aggacagtgc agcgcaggct ccccacaccg gagcatgtca gcagcatcat
     1501 cggtgtactg acagcaaaac catcccgtaa acgtcgtaaa aaccgtacag agccttaccg
     1561 tatcattgat acattccagc gccagttggc aacagcgcag acatcactgg atctgcttcc
     1621 gcaatatgat acagatatcg aatcccgggc aaacgaactg tggaaaaagg ctgtgcacac
     1681 tatcaataac acaaccaggg aagaacgccg ggtatgttat atcacactga atcgtccgga
     1741 agaggaacac tccggtcctg aagatatacc ttcagtgaag gcgattctga atactctgct
     1801 ccccctggag atgctgttac ctgtaccgga tctaactgca tccccgcccc ataaaaaaaa
     1861 tgtcgctcag acaccatcag gtggaaaaca agagagtcgt aaaaaaagat tctgataaca
     1921 cctgtatggc tgattttaat aacaaccttt acgcgattat taatctcctc cgctcaccat
     1981 agtaagcgga ggattcagga tttcaatcca tcaccagagg tacaataaat gtgtattagt
     2041 agcccggaaa caaactcatg gtctgttatt tatcgtaaaa attccggaga agatatcaat
     2101 atcacttccc tgacgtttaa aaatagtctt ctggcagccc ggacactgat ggtgcccgaa
     2161 aactatatga tatgcatact cagaaacggg gagagggtca gacgatggga cagagaaata
     2221 cttgcaggct caaacagatg gtacaagtgt tctcccgata atttcgaaat actgggtaag
     2281 ctccccataa taaataaagt cacaaaactg ataaaatcat aatctacgga ttttaatcag
     2341 ttaagctaaa tcaggatgaa cgctttgatt atgaattata catgaaaaca aatatgggca
     2401 gcacaaaaaa tggtgccagg gttccccacg ttcgtaatag attcacttct atggatattg
     2461 gtatgaattc ccagatccca ctctattgta aacaagacat ttttatcttt tatattcaat
     2521 ggcttatttt cctgctaatt ggtaatacca tgaaaaatac catgctcaga aaaggcttaa
     2581 caatattttg aaaaattgcc tactgagcgc tgccgcacag ctccataggc cgctttcctg
     2641 gctttgcttc cagatgtatg ctcttctgct cctgcagcta atggatcacc gcaaacaggt
     2701 tactcgcctg gggattccct ttcgacccga gcatccgtat gatactcatg ctcgattatt
     2761 attattatag aagcccccat gaataaatcg ctcatcattt tcggcatcgt caacataacc
     2821 tcggacagtt tctccgatgg aggccggtat ctggcgccag acgcagccat tgcgcaggcg
     2881 cgtaagctga tggccgaggg ggcagatgtg atcgacctcg gtccggcatc cagcaatccc
     2941 gacgccgcgc ctgtttcgtc cgacacagaa atcgcgcgta tcgcgccggt gctggacgcg
     3001 ctcaaggcag atggcattcc cgtctcgctc gacagttatc aacccgcgac gcaagcctat
     3061 gccttgtcgc gtggtgtggc ctatctcaat gatattcgcg gttttccaga cgctgcgttc
     3121 tatccgcaat tggcgaaatc atctgccaaa ctcgtcgtta tgcattcggt gcaagacggg
     3181 caggcagatc ggcgcgaggc acccgctggc gacatcatgg atcacattgc ggcgttcttt
     3241 gacgcgcgca tcgcggcgct gacgggtgcc ggtatcaaac gcaaccgcct tgtccttgat
     3301 cccggcatgg ggttttttct gggggctgct cccgaaacct cgctctcggt gctggcgcgg
     3361 ttcgatgaat tgcggctgcg cttcgatttg ccggtgcttc tgtctgtttc gcgcaaatcc
     3421 tttctgcgcg cgctcacagg ccgtggtccg ggggatgtcg gggccgcgac actcgctgca
     3481 gagcttgccg ccgccgcagg tggagctgac ttcatccgca cacacgagcc gcgccccttg
     3541 cgcgacgggc tggcggtatt ggcggcgctg aaagaaaccg caagaattcg ttaactgcac
     3601 attcgggata tttctctata ttcgcgcttc atcagaaaac tgaaggaacc tccattgaat
     3661 cgaactaata ttttttttgg tgaatcgcat tctgactggt tgcctgtcag aggcggagaa
     3721 tctggtgatt ttgtttttcg acgtggtgac gggcatgcct tcgcgaaaat cgcacctgct
     3781 tcccgccgcg gtgagctcgc tggagagcgt gaccgcctca tttggctcaa aggtcgaggt
     3841 gtggcttgcc ccgaggtcat caactggcag gaggaacagg agggtgcatg cttggtgata
     3901 acggcaattc cgggagtacc ggcggctgat ctgtctggag cggatttgct caaagcgtgg
     3961 ccgtcaatgg ggcagcaact tggcgctgtt cacagcctat cggttgatca atgtccgttt
     4021 gagcgcaggc tgtcgcgaat gttcggacgc gccgttgatg tggtgtcccg caatgccgtc
     4081 aatcccgact tcttaccgga cgaggacaag agtacgccgc agctcgatct tttggctcgt
     4141 gtcgaacgag agctaccggt gcggctcgac caagagcgca ccgatatggt tgtttgccat
     4201 ggtgatccct gcatgccgaa cttcatggtg gaccctaaaa ctcttcaatg cacgggtctg
     4261 atcgaccttg ggcggctcgg aacagcagat cgctatgccg atttggcact catgattgct
     4321 aacgccgaag agaactgggc agcgccagat gaagcagagc gcgccttcgc tgtcctattc
     4381 aatgtattgg ggatcgaagc ccccgaccgc gaacgccttg ccttctatct gcgattggac
     4441 cctctgactt ggggttgatg ttcatgccgc ctgtttttcc tgctcattgg cacgtttcgc
     4501 aacctgttct cattgcggac accttttcca gcctcgtttg gaaagtttca ttgccagacg
     4561 ggactcctgc aatcgtcaag ggattgaaac ctatagaaga cattgctgat gaactgcgcg
     4621 gggccgacta tctggtatgg cgcaatggga ggggagcagt ccggttgctc ggtcgtgaga
     4681 acaatctgat gttgctcgaa tatgccgggg agcgaatgct ctctcacatc gttgccgagc
     4741 acggcgacta ccaggcgacc gaaattgcag cggaactaat ggcgaagctg tatgccgcat
     4801 ctgaggaacc cctgccttct gcccttctcc cgatccggga tcgctttgca gctttgtttc
     4861 agcgggcgcg cgatgatcaa aacgcaggtt gtcaaactga ctacgtccac gcggcgatta
     4921 tagccgatca aatgatgagc aatgcctcgg aactgcgtgg gctacatggc gatctgcatc
     4981 atgaaaacat catgttctcc agtcgcggct ggctggtgat agatcccgtc ggtctggtcg
     5041 gtgaagtggg ctttggcgcc gccaatatgt tctacgatcc ggctgacaga gacgaccttt
     5101 gtctcgatcc tagacgcatt gcacagatgg cggacgcatt ctctcgtgcg ctggacgtcg
     5161 atccgcgtcg cctgctcgac caggcgtacg cttatgggtg cctttccgca gcttggaacg
     5221 cggatggaga agaggagcaa cgcgatctag ctatcgcggc cgcgatcaag caggtgcgac
     5281 agacgtcata ctagatatca agcgacttct cctatcccct gggaacacat caatctcacc
     5341 ggagaatatc gctggccaaa gccttagcgt aggattccgc cccttcccgc aaacgacccc
     5401 aaacaggaaa cgcagctgaa acgggaagct caacacccac tgacgcatgg gttgttcagg
     5461 cagtacttca tcaaccagca aggcggcact ttcggccatc cgccgcgccc cacagctcgg
     5521 gcagaaaccg cgacgcttac agctgaaagc gaccaggtgc tcggcgtggc aagactcgca
     5581 gcgaacccgt agaaagccat gctccagccg cccgcattgg agaaattctt caaattcccg
     5641 ttgcacatag cccggcaatt ccttttcctg ccagacatgc tgattaattt tgcccttatt
     5701 aacatcacag acagaaagaa cgagggtacc aataccattg atggtaactg gcaggccgat
     5761 gaaggacgcc gttaccggga taatgtaaga atctattttt gagtatgtca acataacctg
     5821 aggcaaagta cctcaggttc cagtgtagta atcagccaac ctgacagtct tctgttagcc
     5881 ctacagatat gtcgtggagc taaccgcgca tgtcaaagcc ggatttttga aaatctctat
     5941 aaacttccgg attgttgaag gccgggaatt tagctttatg ggctgctgac tttatcgctt
     6001 cgcaataggc tttatcgcca ttactggttg atatttttaa cgccgtgcca tcccgggaga
     6061 attccatatg caacctgcat tttttccttt tccatttttg cggatcatca agtttagcat
     6121 taattgcatc cctgattccc cgtgcttgct ccccccattc atcctgatca tcccaacgtc
     6181 ctgaactgca actacccagg gctgtagttt tatggcaatt tgaaggatgt aatggcgcac
     6241 atcccgccac aaagctgact aaaatagcca gcataacgat tttctttacc cccatatatt
     6301 gctcctcaat ccatatagat ctcagtacca tagctctgtc agagcaaaga aaatcttaac
     6361 taatacaagt taagattttt aatatccact cctcatccct acattccagg tgatggtggc
     6421 gtccactcat tatcctgaac tggagcataa gatgcgccat gccccccgga tatgcttttc
     6481 tgttgcctga tatattcgtg gtagtactgt tcactacgct gacgatgtaa atccacatcc
     6541 gggcggctga ttacaaagtg tccacttccg gttattccag ctgcattatg ccaggccacc
     6601 atttccgcaa atacatcatt aaactgttga tatgagcctt ccggttcaaa acccattctt
     6661 ggctcacgat caaacaatac aggagcaatc agattcagct tatccaccag ttcatttctg
     6721 tcctgatgat ggtagaacac cacacggtcc ccataatcca cacagactaa ctcgccattc
     6781 ctccgatcac gaaaacgcac actgccatct ttctgcagac gggcttcgag aacgccagtg
     6841 tcttcataaa gtggagttcc tcccggttca cagagaatca cgcagcggtc ggcattcgtt
     6901 gttcgtcgct tgtctttgcg acgatcacga taatcccagc cacgtaactg tgataatgca
     6961 gctctgtctc cctgtaccgc ctgttgctcc acccactgcc ggtaggacaa aggataccac
     7021 ttaccctccg caatcagtga caaccgctct tccttcacac tctccttcag cctgatcagc
     7081 gcctgcatac gctgcacttc tgcaatatgg taatgcagct tacgcaattg tggatcccgg
     7141 aactgcacac ggatatacgc cttgcgccga cggcaggcag catgaatttc ccgtaaccgt
     7201 tctccgtaac gtaagtcggg tttacgccag tgctccttcc atgcaagata acgcgcgcgc
     7261 aaatcttccc gcgcagctgc acgggcttca cggcgttcac gacgcagtac aggatcgcgc
     7321 cggaagccga gctccacttc atcgctggca gcaagttccg gattgtagcg acattcaggc
     7381 ttcacccgtt caaatatatc tgcacctgca atctcaaatg gccctgcctg cggctcagcc
     7441 ctggataacg tcaaatccgg gtggatactg ctggccttca ctggtgtgag ctcatgatta
     7501 aacgcatcca caatcacaag accatgatgc tgtttctgca acataagccc tttacgcgca
     7561 aacatctcat ggcacgcctg ccagctgaaa ttcccgcccg cgatcatatc ttcaatcaac
     7621 gactcacact gaacagtcgt gtacccccac agactttcct tatcggaaaa cagttcgcgc
     7681 tttattgctc cctgtggacc tgtccccatc cgaatacgat accattcgta atcactgaca
     7741 gcctgagtac caagattacg tccccggaat cgttctccgg gtataacctg agtaaaaata
     7801 tccttcggta caggctgcca tccaccatcc agatcctgca tataatccag cgtcattaac
     7861 ggccacacac tgtctgcacg tactggtgtc tgttgtttat caaatccatc atgcagtacc
     7921 agatgcccgt gctggatccc catccataat ccagcctttg ccagagttct gtgcacatcc
     7981 aggcaacttt ccagctcacc atttctcgcc atttccggta agcgttcggc aaaatgacgg
     8041 caggcgtact gtttcagact ctccgtttct gccacttttt ccggccggat atcaacgttt
     8101 atggcttccg gatcataacg accgggtgtc cccacctgac tgaaaatatc tgtcggtaca
     8161 ggctgatatt cccccagttt ccgccccagt ttttcagctg tccatgacgg tccgaacgaa
     8221 ctgagtgcaa caccttcccg cgctctgtcc cagccatcct tcaccaccag ttccccttcc
     8281 tgcatcgtga tatacagacc atcactggcc agccgtttat gcaaagacaa ccagtcttgt
     8341 gcgggctctt cacgcagccc ggcaatcgac atctgcgcaa tatattccct gaacgtctgc
     8401 tttttcccgc gtcgccaggc attacggcgc tcacgtacca gcgctgtttt gcgcacaata
     8461 cggttgccgg gtgcatgcac aaagcagcca ttatcaggcg cgaatccgtg cttaagttca
     8521 agttcacgac atgcacggct gagtttttcc tggctccagg aaagccagtt aatataacca
     8581 gtttcaggat gtacacgatt cacggcaaca tgcacatgca gattatccgt gtccgtatga
     8641 acggcagcca cgtactgatg ttttgagagt tcaagagatt tcagtgtgtg acgcacacag
     8701 tcaaaaagct gctcaggacg cgggctttca tgcgcaggcc aggaaagaat gtagtggaaa
     8761 acggggtctg tatcattacg ggaaaaaaca gctttgtccg ccttgtactg catctcttca
     8821 gcagctgttt ccagtgaatt gcagttatgg aaacaggtta cgccatagaa atttacccac
     8881 tgactgccgt ccttcatcac atcaatcagg gagacaaatt tttcgttacg caggcgtgtt
     8941 gcataatcaa caagccgtct gaaacggttg cggtgaggga catcactctt gatttcgctg
     9001 tcttctgtga gctcattttc ctgcacatca tcccgcacag acgtgtacgc aatcaggtct
     9061 tcaaaggaag acctgccgtc acgtcttttg tgcggaataa tggcattcat cagcagttat
     9121 ctcagcgtcc cattaagtct cttcgcagag cattgacata ttcagtaatg gcaacaagaa
     9181 cctctgcata ctccttatca ccagtgcgtt ttccctccac aaacagatgc ttctgaaggc
     9241 ggccaagacg cataatctca tcaatcatac ggtcgtcagt acggcagtta accaccttat
     9301 ccagtgaaga acttcgtata aatgttgaaa ccgtctgtcc acttgactcc gctttttctc
     9361 ttacgatttc agcctcatcc tccgtgaatc tcgccgtgaa ttttacaatt ctcttcctgt
     9421 tctcactgcc cgttcgggtt ttcttctcac tcatggtctg ctccttaccg gggtgtcggg
     9481 gcaaagccct gaccaggtgg ggaatgtctg agtgcgcgtg cgcggtccga cattcccaca
     9541 tcctgtcccg tttttcagtc cattatagcc tgttattttt gtgcatcata gtcttatgca
     9601 gacggcagag gcagggcgca gccctgccag gagtcagttt ttgatgataa aatcaggctt
     9661 caacattaaa cggagagaaa gtgccaatgg cgtctaaaaa attttactca gatgatgaca
     9721 tccagctggc aaaagctgct ttatcggaat tgccagacct caccgctcaa cggaaaacat
     9781 tgcgtgattt tcttgatgcc attcgtgatg acatcattat cctggtaagg acaaagggtt
     9841 atacactgac agatgttcgt gacacgctca aaaatgcagg atatgaagca ggagagaaag
     9901 cacttcgtga cattattcgg gaagcagaat cgaaaaaacc atcccgccgt tcttcctcta
     9961 aaacagcacc taaaaagaca gactcaggca ggaaagatgg catcgatatg aacaacaatt
    10021 gatgtgacaa tgtcacattg tgttctgaga ttatgatgat tacagaaaaa aaagccccgg
    10081 cataaatacc tgggcttttc tgtttaatca gactcgtggc ggcaatgcac caggataatg
    10141 ccgtgcaata atatcattca gtttttcaac aaggtcagaa cgcttaaaga caatgtgtac
    10201 ggtccctttc tggtagccac gcaccgta
//
LOCUS       DAEEKQ010000491         5219 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.guided.230, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000491 DAEEKQ010000000
VERSION     DAEEKQ010000491.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5219
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.guided.230"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            836..1915
                     /locus_tag="KLK57_004656"
     CDS             836..1915
                     /locus_tag="KLK57_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HBE0894791.1"
                     /translation="MLLARDLGYRLEGTDAQTLEVKKYKGSFPYICILDEVGAYYTDR
                     IAVEATQVRSLEFSLIMTAQDQERIEGQTSATNTATLMQNAGTKFAGRIVSDDKTART
                     VKNAAGEEARARMGSLQRHDGVMGESWVDGNQITIQMESKIDVQDLIRLNAGEFFTVF
                     QGDVVPSASVYIPDSEKSCDSDPVVINRYISVEAPRLERLRRLVPRTVQRRLPTPEHV
                     SSIIGVLTAKPSRKRRKNRTEPYRIIDTFQRQLATAQTSLDLLPQYDTDIESRANELW
                     KKAVHTINNTTREERRVCYITLNRPEEEHSGPEDIPSVKAILNTLLPLEMLLPVPDLT
                     ASPPHKKNVAQTPSGGKQESRKKRF"
     gene            2779..3594
                     /gene="sul2"
                     /locus_tag="KLK57_004657"
     CDS             2779..3594
                     /gene="sul2"
                     /locus_tag="KLK57_004657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HBE0894792.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            3655..4458
                     /locus_tag="KLK57_004658"
                     /note="aph(3'')-Ib"
     CDS             3655..4458
                     /locus_tag="KLK57_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HBE0894793.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            4458..>5219
                     /locus_tag="KLK57_004659"
     CDS             4458..>5219
                     /locus_tag="KLK57_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(6)-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HBE0894794.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRSLDVDPRRLLAQAYAYGCLSAAWN"
BASE COUNT         1239 a         1401 c         1375 g         1204 t
ORIGIN      
        1 gagcatggat gacctcaccc ggcatgtcat gttttttgcc accaccggtg gcggtaaaac
       61 agaaaccacc tttgcctggc tgctcaaccc gctctgctgg ggaagaggct ttacctttgt
      121 tgacggaaag gcacagaacg ataccacgag gacaatctgg tatctctccc gacgttttgg
      181 cagggaggat gatatcgaag tcatcaactt catgaacggc ggaaaatccc gcagtgaaat
      241 cattcagagc ggcgaaaaat cccgcccaca gtcaaacacc tggaacccgt tcgcattcag
      301 caccgaagcc ttcaccgccg aaaccatgca gtccatgctg ccccagaacg ttcagggcgg
      361 tgaatggcag tcacgggcga tagccatgaa caaggccctg gtctttggca caaaattctg
      421 gtgcgtccgg gaaggtaaaa ccatgtccct gcagatgtta cgggaacata tgacgctgga
      481 agggatggca aaactctatt gccgtggcct tgatgaccag tggccggaag aagctatcgc
      541 accactgcga aactacctgc aggatgtccc gggattcgat atgtcactgg tgagaacgcc
      601 gtcagcctgg actgaggagc cccgtaaaca gcatgcttat ctctccggtc agttctcgga
      661 aacgttcaca acctttgccg aaaccttcgg ggacgtcttt gcggcagatg ccggcgacat
      721 cgatattcgc gacagtattc acagcgaccg cattctgatt gtgatgatcc ctgcactgga
      781 cacatcggct cataccacgt cggcactcgg tcgtatgttc gtcacccagc agagcatgct
      841 cctcgcccgt gacctgggct accggctgga aggcacagat gcacagaccc tggaagtcaa
      901 aaaatacaaa ggcagcttcc cctatatctg tatccttgac gaagtcggtg cctactacac
      961 agaccgcatt gctgtggagg ccacccaggt acgttcactg gagttttccc tgataatgac
     1021 ggcgcaggat caggagcgaa ttgaagggca gacctccgcc accaacaccg ccacactgat
     1081 gcagaacgcc ggcaccaaat tcgccggacg cattgtcagt gacgacaaaa cggcaagaac
     1141 ggtgaaaaat gccgccggtg aagaggcacg ggccagaatg ggcagcctgc agcgccacga
     1201 tggtgtgatg ggtgagtcct gggtggacgg taaccagatc accatccaga tggaaagcaa
     1261 aatcgacgtg caggatttga tcagactgaa tgctggtgag ttctttaccg ttttccaggg
     1321 ggatgtggtg ccgtcagcct ctgtgtatat tccggacagc gagaaaagtt gcgacagtga
     1381 tccggtggtc atcaaccgct atatttccgt tgaagccccc cggctggagc gtctccgccg
     1441 tctcgtcccc aggacagtgc agcgcaggct ccccacaccg gagcatgtca gcagcatcat
     1501 cggtgtactg acagcaaaac catcccgtaa acgtcgtaaa aaccgtacag agccttaccg
     1561 tatcattgat acattccagc gccagttggc aacagcgcag acatcactgg atctgcttcc
     1621 gcaatatgat acagatatcg aatcccgggc aaacgaactg tggaaaaagg ctgtgcacac
     1681 tatcaataac acaaccaggg aagaacgccg ggtatgttat atcacactga atcgtccgga
     1741 agaggaacac tccggtcctg aagatatacc ttcagtgaag gcgattctga atactctgct
     1801 ccccctggag atgctgttac ctgtaccgga tctaactgca tccccgcccc ataaaaaaaa
     1861 tgtcgctcag acaccatcag gtggaaaaca agagagtcgt aaaaaaagat tctgataaca
     1921 cctgtatggc tgattttaat aacaaccttt acgcgattat taatctcctc cgctcaccat
     1981 agtaagcgga ggattcagga tttcaatcca tcaccagagg tacaataaat gtgtattagt
     2041 agcccggaaa caaactcatg gtctgttatt tatcgtaaaa attccggaga agatatcaat
     2101 atcacttccc tgacgtttaa aaatagtctt ctggcagccc ggacactgat ggtgcccgaa
     2161 aactatatga tatgcatact cagaaacggg gagagggtca gacgatggga cagagaaata
     2221 cttgcaggct caaacagatg gtacaagtgt tctcccgata atttcgaaat actgggtaag
     2281 ctccccataa taaataaagt cacaaaactg ataaaatcat aatctacgga ttttaatcag
     2341 ttaagctaaa tcaggatgaa cgctttgatt atgaattata catgaaaaca aatatgggca
     2401 gcacaaaaaa tggtgccagg gttccccacg ttcgtaatag attcacttct atggatattg
     2461 gtatgaattc ccagatccca ctctattgta aacaagacat ttttatcttt tatattcaat
     2521 ggcttatttt cctgctaatt ggtaatacca tgaaaaatac catgctcaga aaaggcttaa
     2581 caatattttg aaaaattgcc tactgagcgc tgccgcacag ctccataggc cgctttcctg
     2641 gctttgcttc cagatgtatg ctcttctgct cctgcagcta atggatcacc gcaaacaggt
     2701 tactcgcctg gggattccct ttcgacccga gcatccgtat gatactcatg ctcgattatt
     2761 attattatag aagcccccat gaataaatcg ctcatcattt tcggcatcgt caacataacc
     2821 tcggacagtt tctccgatgg aggccggtat ctggcgccag acgcagccat tgcgcaggcg
     2881 cgtaagctga tggccgaggg ggcagatgtg atcgacctcg gtccggcatc cagcaatccc
     2941 gacgccgcgc ctgtttcgtc cgacacagaa atcgcgcgta tcgcgccggt gctggacgcg
     3001 ctcaaggcag atggcattcc cgtctcgctc gacagttatc aacccgcgac gcaagcctat
     3061 gccttgtcgc gtggtgtggc ctatctcaat gatattcgcg gttttccaga cgctgcgttc
     3121 tatccgcaat tggcgaaatc atctgccaaa ctcgtcgtta tgcattcggt gcaagacggg
     3181 caggcagatc ggcgcgaggc acccgctggc gacatcatgg atcacattgc ggcgttcttt
     3241 gacgcgcgca tcgcggcgct gacgggtgcc ggtatcaaac gcaaccgcct tgtccttgat
     3301 cccggcatgg ggttttttct gggggctgct cccgaaacct cgctctcggt gctggcgcgg
     3361 ttcgatgaat tgcggctgcg cttcgatttg ccggtgcttc tgtctgtttc gcgcaaatcc
     3421 tttctgcgcg cgctcacagg ccgtggtccg ggggatgtcg gggccgcgac actcgctgca
     3481 gagcttgccg ccgccgcagg tggagctgac ttcatccgca cacacgagcc gcgccccttg
     3541 cgcgacgggc tggcggtatt ggcggcgctg aaagaaaccg caagaattcg ttaactgcac
     3601 attcgggata tttctctata ttcgcgcttc atcagaaaac tgaaggaacc tccattgaat
     3661 cgaactaata ttttttttgg tgaatcgcat tctgactggt tgcctgtcag aggcggagaa
     3721 tctggtgatt ttgtttttcg acgtggtgac gggcatgcct tcgcgaaaat cgcacctgct
     3781 tcccgccgcg gtgagctcgc tggagagcgt gaccgcctca tttggctcaa aggtcgaggt
     3841 gtggcttgcc ccgaggtcat caactggcag gaggaacagg agggtgcatg cttggtgata
     3901 acggcaattc cgggagtacc ggcggctgat ctgtctggag cggatttgct caaagcgtgg
     3961 ccgtcaatgg ggcagcaact tggcgctgtt cacagcctat cggttgatca atgtccgttt
     4021 gagcgcaggc tgtcgcgaat gttcggacgc gccgttgatg tggtgtcccg caatgccgtc
     4081 aatcccgact tcttaccgga cgaggacaag agtacgccgc agctcgatct tttggctcgt
     4141 gtcgaacgag agctaccggt gcggctcgac caagagcgca ccgatatggt tgtttgccat
     4201 ggtgatccct gcatgccgaa cttcatggtg gaccctaaaa ctcttcaatg cacgggtctg
     4261 atcgaccttg ggcggctcgg aacagcagat cgctatgccg atttggcact catgattgct
     4321 aacgccgaag agaactgggc agcgccagat gaagcagagc gcgccttcgc tgtcctattc
     4381 aatgtattgg ggatcgaagc ccccgaccgc gaacgccttg ccttctatct gcgattggac
     4441 cctctgactt ggggttgatg ttcatgccgc ctgtttttcc tgctcattgg cacgtttcgc
     4501 aacctgttct cattgcggac accttttcca gcctcgtttg gaaagtttca ttgccagacg
     4561 ggactcctgc aatcgtcaag ggattgaaac ctatagaaga cattgctgat gaactgcgcg
     4621 gggccgacta tctggtatgg cgcaatggga ggggagcagt ccggttgctc ggtcgtgaga
     4681 acaatctgat gttgctcgaa tatgccgggg agcgaatgct ctctcacatc gttgccgagc
     4741 acggcgacta ccaggcgacc gaaattgcag cggaactaat ggcgaagctg tatgccgcat
     4801 ctgaggaacc cctgccttct gcccttctcc cgatccggga tcgctttgca gctttgtttc
     4861 agcgggcgcg cgatgatcaa aacgcaggtt gtcaaactga ctacgtccac gcggcgatta
     4921 tagccgatca aatgatgagc aatgcctcgg aactgcgtgg gctacatggc gatctgcatc
     4981 atgaaaacat catgttctcc agtcgcggct ggctggtgat agatcccgtc ggtctggtcg
     5041 gtgaagtggg ctttggcgcc gccaatatgt tctacgatcc ggctgacaga gacgaccttt
     5101 gtctcgatcc tagacgcatt gcacagatgg cggacgcatt ctctcgttcg ctggacgtcg
     5161 atccgcgtcg cctgctcgcc caggcgtacg cttatgggtg cctttccgca gcttggaac
//
LOCUS       DAEEKQ010000492         4292 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.guided.257, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000492 DAEEKQ010000000
VERSION     DAEEKQ010000492.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4292
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.guided.257"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            46..1179
                     /locus_tag="KLK57_004660"
     CDS             46..1179
                     /locus_tag="KLK57_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HBE0894795.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLTPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            1268..1801
                     /gene="blc"
                     /locus_tag="KLK57_004661"
     CDS             1268..1801
                     /gene="blc"
                     /locus_tag="KLK57_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HBE0894796.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDREMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(1798..2115)
                     /gene="sugE"
                     /locus_tag="KLK57_004662"
     CDS             complement(1798..2115)
                     /gene="sugE"
                     /locus_tag="KLK57_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HBE0894797.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(2290..2436)
                     /gene="ecnB"
                     /locus_tag="KLK57_004663"
     CDS             complement(2290..2436)
                     /gene="ecnB"
                     /locus_tag="KLK57_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HBE0894798.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(2547..2672)
                     /gene="ecnA"
                     /locus_tag="KLK57_004664"
     CDS             complement(2547..2672)
                     /gene="ecnA"
                     /locus_tag="KLK57_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HBE0894799.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(2724..3290)
                     /gene="efp"
                     /locus_tag="KLK57_004665"
     CDS             complement(2724..3290)
                     /gene="efp"
                     /locus_tag="KLK57_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HBE0894800.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            3332..>4292
                     /gene="epmB"
                     /locus_tag="KLK57_004666"
     CDS             3332..>4292
                     /gene="epmB"
                     /locus_tag="KLK57_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HBE0894801.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPGELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPILRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVP"
BASE COUNT         1086 a         1099 c         1082 g         1025 t
ORIGIN      
        1 tccggcccgc ctatggcggg ccgttttgta tggaaaccag accttatgtt caaaacgacg
       61 ctctgcgcct tattaattac cgcctcttgc tccacatttg ctgcccctca acaaatcaac
      121 gatattgtgc atcgcacaat taccccgctt atagagcaac aaaagatccc cggtatggcg
      181 gtggcggtaa tttatcaggg taaaccttat tactttacct ggggctatgc ggacatcgcc
      241 aaaaagcagc ccgtcacaca gcaaacgttg tttgagttag gttcggtcag caaaacattt
      301 acgggcgtgc ttggtggcga cgctattgct cgaggggaaa tcaagttaag cgatcccgca
      361 acaaaatact ggcctgaact tactgctaaa cagtggaatg ggatcacact attacatctt
      421 gcgacctaca ccgctggcgg cctgccattg caggtgccgg atgaagtgaa atcctcaagc
      481 gacttgctgc gcttctatca aaactggcag cctgcatggg ctccaggaac acaacgtctg
      541 tatgccaact ccagtatcgg tttgttcggc gcactggctg tgaagccgtc tggtttgagt
      601 tttgagcagg cgatgcaaac tcgtgtcttc cagccactca aactcaacca tacgtggatt
      661 aatgtacctc ccccagaaga aaagaattac gcctggggat atcgcgaagg taaggcagtg
      721 catgtttcgc caggggcgtt agatgctgaa gcttatggtg tgaagtcgac cattgaagat
      781 atggcccgct gggtgcaaag caatttaacc ccccttgata tcaatgagaa aacgcttcaa
      841 caagggatac aactggcaca atctcgctac tggcaaaccg gcgatatgta tcagggcctg
      901 ggctgggaaa tgctggactg gccggtcaat cctgacagca tcattaacgg cagtgacaat
      961 aaaattgcac tggcagcacg cccggtaaaa gcgattaagc ccccaactcc tgcagtacgc
     1021 gcatcatggg tacataaaac gggggcgacc ggcggatttg gtagctatgt cgcgtttatt
     1081 ccagaaaaag agctgggtat cgtgatgctg gctaacaaaa actatcccaa tccagcgaga
     1141 gtcgccgccg cctgtcagat tctcaacgct ctacagtaaa atttcatcgg gtccgaattt
     1201 tcggaccttt tctgcgcttt tccttgctgt catctacact tagaaaaaaa ccagtaagga
     1261 aacatttatg cgcctgctcc ctctcgttgc cgcagcgaca gctgcatttc tggtcgttgc
     1321 ctgcagttct cctacgccgc cgcgtggcgt gacagtagtg aataatttcg acgctaaacg
     1381 ctatctcggt acctggtatg agattgcccg ttttgatcac cgttttgaac gtggactgga
     1441 aaaagtcacc gcaacataca gtctgcgtga tgacggcggc ctgaatgtca tcaataaagg
     1501 ctataaccct gacagagaaa tgtggcagca gagtgaaggg aaagcgtact ttaccggcgc
     1561 accaacacgc gctgcgctga aagtgtcatt ctttggtcct ttctatggcg gttataacgt
     1621 cattgcactt gatcgggaat accgccatgc gctggtttgc ggcccggacc gcgactacct
     1681 gtggatactc tcccgcacgc caaccatttc tgacgaagtg aaacaggaga tgctggcagt
     1741 cgcgacccgg gaagggtttg atgtcagtaa gtttatttgg gtacagcagc ccggtagtta
     1801 gtgagtgctg agtttcagac caataatccc caatacgatt aacgccagac tcgccaggcg
     1861 catcgggtta gcggactcac cgagcagcac aatgccggtg atggccgcgc cgactgcgcc
     1921 aatacccgtc cacacggcat aagccgtccc taccggtaac gatttcatcg cccaggcaag
     1981 tagcgccata ctgactatca tcgccgtcac agtaataaca cttggcgtca aacgactaaa
     2041 gccgtgggta tatttcaggc caacggccca taccacttcc agcagaccag caataactaa
     2101 gataatccag gacatatcag gctccggaga attggggcca tccccggtga aaagaggcgt
     2161 ttgcaggtcg tcctgcaaag ctgatggttg aacggatatt ttgccaggtg gcaggggaat
     2221 ttcaactatt acctttgtga ctatcgcaaa aaaacaaacg gcacgacata actgtcgtac
     2281 cgttattgct tattgctgcg ctttcgttgc tgcaccagaa atcgcgttac cgccatcaga
     2341 aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt aatactgttg aaagcaccag
     2401 aacagaaaag atcgctgcaa ttgtcttctt caccataacg tcttcctttt aggtaatgtt
     2461 atttatgttt gcctatagca aattaataat agacaacatc gccaaaaatg atggaaatct
     2521 gatgattttt cggaagagaa gaaaaattag ctggcagcgc gggagatgga gttgccgaga
     2581 tgtttgatgt cttcgccgaa accgcgagcg gtgttacagc ccgtgagcag cgtgctggca
     2641 agcaaaacaa gaacgataag gcgtttcatc atctctgacc tgaataagtg atggtgcagc
     2701 ctgcaggccg caccacaacc gcattacttc acgcgagaga cgtattcacc agagcgggta
     2761 tccactttga tgacttcgcc gatttgtacg aacagcggaa ctttaaccac agcgccagta
     2821 gacagggtag ccggtttgcc gccagtacct gcggtatcac ctttcaggcc cggatcggta
     2881 tcaacgattt ccagttcaac gaagttcggc ggagtaacgg agatcggctg accattccac
     2941 agagttacga tacactctgc ctgatccagc agccatttag cgttgtcgcc aattgctttt
     3001 gcatcagcag acagctgctc gaaagtttcg ttgttcatga agtgccagaa ctcaccgtcg
     3061 ttgtacaggt aagtcaggtt catatcgaca acatcagcgc cttcagcgga atcagtagat
     3121 ttgaaggttt tttctacgcg agtaccggtc agcagacgac gcagtttaac gcgagcaaat
     3181 gcctggcctt tacccggttt tacgaattca ctcgcttcaa ccgcgtaagg ttcgccgtct
     3241 aacatgattt taagaccagc acgaaaatcg ttgctatagt acgttgccat aaggccctct
     3301 gaaatttgtt aattggtagc taagccacaa aatggcgcat attgtaaccc taaatacccc
     3361 atccagagaa gattggttaa cgcaacttgc cgatgttgtg accgatcctg gtgaacttct
     3421 gcgtcttttg aatatagacg cggacgaaaa actgttggcc ggacgcagcg ccaaaaagct
     3481 gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg gagaaaggca atccggacga
     3541 tcctattttg cgtcaggtac ttacctcgca agatgagttt gtcgtcgcgt ccggattctc
     3601 caccgaccca ctggaagaac agcacagcgt agtgcctggt ttgttgcata aataccacaa
     3661 ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat tgccgctatt gcttccgtcg
     3721 ccacttcccc tatgccgaaa atcagggcaa caagcgtaac tggcagactg cacttgagta
     3781 tgttgctgcg catccggaac tggacgagat gattttctcc ggcggcgatc cgctgatggc
     3841 gaaagatcac gagctggact ggttgctcac acaactggaa gccatcccgc atatcaaacg
     3901 tctgcggatt cacagccgtc tgccgattgt gatcccggca cgtatcaccg aggcgctggt
     3961 tgaacgcttt gcccgttcta cgctgcaaat cttgctggtg aatcacatca atcatgccaa
     4021 tgaggtagat gaaacgttcc gtcaggcgat ggctaaattg cgccgtgtcg gtgtcaccct
     4081 gcttaaccag agcgttctgt tacgtggtgt gaacgataac gcacaaacgt tggcaaacct
     4141 gagtaatgcg ttgttcgatg ccggcgtgat gccgtattac ctgcatgtgc tagataaagt
     4201 acagggcgcg gcgcatttta tggtgagtga tgacgaagca cggcagatta tgcgtgagtt
     4261 gctgacattg gtgtcgggat atctggtgcc ga
//
LOCUS       DAEEKQ010000493         4106 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.guided.264, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000493 DAEEKQ010000000
VERSION     DAEEKQ010000493.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4106
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.guided.264"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..892)
                     /gene="pepD"
                     /locus_tag="KLK57_004667"
     CDS             complement(<1..892)
                     /gene="pepD"
                     /locus_tag="KLK57_004667"
                     /EC_number="3.4.13.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-Ala-His dipeptidase"
                     /protein_id="HBE0894802.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKSVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKN"
     gene            1153..1611
                     /gene="gpt"
                     /locus_tag="KLK57_004668"
     CDS             1153..1611
                     /gene="gpt"
                     /locus_tag="KLK57_004668"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HBE0894803.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            1703..2947
                     /gene="frsA"
                     /locus_tag="KLK57_004669"
     CDS             1703..2947
                     /gene="frsA"
                     /locus_tag="KLK57_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HBE0894804.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     IQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            3005..3406
                     /gene="crl"
                     /locus_tag="KLK57_004670"
     CDS             3005..3406
                     /gene="crl"
                     /locus_tag="KLK57_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HBE0894805.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(3445..>4106)
                     /locus_tag="KLK57_004671"
     CDS             complement(3445..>4106)
                     /locus_tag="KLK57_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin"
                     /protein_id="HBE0894806.1"
                     /translation="GGDSSAQTDNFMTKRASGLATYRNTDFFGVIDGLNLTLQYQGKN
                     ENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQNLQSRGTGKRAEAWATG
                     LKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQFDFGLRPSLGYVLSKGK
                     DIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNKLNINNDDIVAVGMTYQ
                     F"
BASE COUNT          988 a         1009 c         1046 g         1063 t
ORIGIN      
        1 gatttttctc tttctctgcc agctcgtttt tcaggatctc ctgataggta ttcaccagag
       61 atttcaggac gtcgacttta tcagctgcga cagcaatggt cgcaaaggct tcacgcggga
      121 tggcgttacg cagtgtgccg ccgttgaaat cgataaggcg cagatccagt tcttctgcat
      181 gacccgctag gaagcgcacc agcagtttgt tggcattacc caggccaacg tggatttccc
      241 cgccggagtg accccctttc agacctttta aggttaactt gaaggtttca aaaccagctg
      301 gaaccgcttc acgatctaaa tgcaggttgg aggtgaagtc gatacccccc gcacaaccca
      361 tgtagatttc accttcttct tcggagtcgg tgttaatcag aatatcagcc tgcaaccagt
      421 tgctctgtaa gccgaacgca ccgtccatac cggcttcttc ggtcatggtc agcagcactt
      481 ccagcgggcc gtgaaccacg ttttcgtcag ccagaaccgc cagcgcagac gccataccaa
      541 tgccgttatc tgcacccagc gtggtgccgc gcgctttaac ccattcgcca tcaatataag
      601 gctggatagg atctttcgtg aagtcatgca cggtgtcgtt atttttctgc ggcaccatat
      661 cgaggtgggc ctgtaagacg accgatttac gattttccat acctgcggta gcaggtttac
      721 gaatcaggat attacctacc tgatcgcgtt cgacatggaa acctttctct tttgcccaac
      781 caacaatgta ttcagcgagt tgctcttcat gataggacgg gtgaggaata gaacagattt
      841 tggcaaaaat atcccacagc ggctgtggag ataattgaga cagttcagac acgttaagtc
      901 tccttgtcga tcacccgcaa aacagtattg caggtcacag ggttagcaga aaatgttgtc
      961 aacacaagac aggcttgcga gatatgtttg agaataccac tttatcccgc gtcagggaga
     1021 ggcagtgcgt aaaaagacgc ggactcatgt gaaatactgg tttttagtgc gccagatctc
     1081 tataatctcg cgcaacctat tttcccctcg aacacttttt aagccgtaga taaacaggct
     1141 gggacacttc acatgagcga aaaatacatc gtcacctggg acatgttgca gatccatgca
     1201 cgtaaactcg caagccgact gatgccttct gaacaatgga aaggcattat tgccgtaagc
     1261 cgtggcggtc tggtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat
     1321 accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctaaaacgc
     1381 gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact
     1441 gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg
     1501 gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa
     1561 cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa
     1621 cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt
     1681 aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc
     1741 ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc
     1801 gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg
     1861 tctgatgtgg atctggcgcg gcattgaccc acgtgaaatc ctcgacgtcc aggcgcgtat
     1921 tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg
     1981 tggtggcaac tggatttatg agtgggccat ccaggcgatg gtgtggcaac aaaaagcctg
     2041 tgcggaagaa gatccgcaac tcagtggtcg tcactggctg catgcggcta cgttgtacaa
     2101 cattgctgcc tatcctcatc tgaaaggaga tgacctggcc gagcaagcgc aggctttgtc
     2161 aaaccgtgcc tatgaagaag ccgcccagcg tctgccgggc acgatgcggc agatggagtt
     2221 taccgtaccc ggcggtgcgc ccatcaccgg ctttttgcat atgccgaaag gcgatggccc
     2281 gttcccgaca gtattaatgt gtggtggtct ggatgcgatg cagacggact attacagcct
     2341 gtatgaacgt tattttgcgc cgcgcggcat tgcgatgctg actattgata tgccgtcggt
     2401 gggcttttca tcaaaatgga agctcaccca ggactccagc ctgttgcatc agcacgtttt
     2461 aaaggctctg cctaacgtac cgtgggtgga tcacactcgc gtcgcggcct ttggtttccg
     2521 tttcggcgct aacgttgccg tgcgtctggc atatcttgaa tcgccgcgtc tgaaagcggt
     2581 tgcctgtctt ggtccggtag ttcataccct gttgagtgat tttaagtgcc agcaacaggt
     2641 gccggaaatg tatcttgacg ttctggcgag tcgtttgggg atgcatgatg cttccgacga
     2701 agcgttacgc gtggagctga atcgctattc attaaaagtg caaggattgc tggggcgtcg
     2761 ctgcccaacg ccaatgttat caggctactg gaagaacgat ccgttcagcc cggaagagga
     2821 ctcacgctta atcacctcat catctgctga cggtaaatta ttagagatcc catttaaccc
     2881 ggtgtatcgg aattttgaca aaggtcttca ggaaatcacc ggctggatcg aaaaacgctt
     2941 gtgttaaaaa tttgctaaaa tttgccaatt tggtaaaaca gttgcatcac aacaggagat
     3001 tgcaatgacg ttaccgagtg gacacccgaa gagcagattg atcaaaaaat ttaccgcact
     3061 aggcccgtat attcgtgaag gtaagtgcga agataatcga ttctttttcg attgtctggc
     3121 tgtatgcgtc aacgtgaaac cggcaccgga agtgcgtgag ttctggggct ggtggatgga
     3181 gcttgaagcg caggaatccc gttttaccta cagttaccag tttggtctgt tcgataaagc
     3241 aggcgactgg aagagtgttc cggtaaaaga cactgaagtg gttgaacgac tggagcacac
     3301 cctacgtgag tttcacgaga agctgcgtga actgctgacg acgctgaatc tgaagctgga
     3361 accggcggat gattttcgtg atgaaccggt gaagttaacg gcgtgagtga aatgtgccgg
     3421 atgtatcaca tccggcaata tccattaaaa ctgataggtc atgccaaccg cgacaatatc
     3481 atcattatta atattcaatt tgttatcgct atccagttgg ttgattttat aatcaacaaa
     3541 cgctgacata tttttgttga aataatacgt agcaccgacg tcgatataat tgaccagatc
     3601 ttcatcaccg ataccttcaa tatctttccc tttcgataag acataaccca gcgatggacg
     3661 cagaccaaag tcaaactggt attgagcgac cgcttcaaag ttctgtgtct tattggcaaa
     3721 gccgccagtt attggcgtca ttttgcgtgt ttcagaatag aaagttgcca gataaatatt
     3781 attggcatcg tatttcagac ctgttgccca tgcttctgca cgcttgcctg tgccacggct
     3841 ttgcaggttc tgctcgttgg tgcgatctga gttggtatag gccccactaa tggcgaaatc
     3901 gctgccgcca aagtcatatg tcaatgacgt gccgaagcca tcgccgtttt gctttttaac
     3961 gtcgcggttt tcgtttttcc cttgatattg cagggttaag ttcaggccat cgataacgcc
     4021 gaagaagtcg gtgttccgat acgtcgccag accgctggcg cgtttggtca taaagttgtc
     4081 ggtctgcgcc gaggaatcgc caccaa
//
LOCUS       DAEEKQ010000494         3406 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.guided.289, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000494 DAEEKQ010000000
VERSION     DAEEKQ010000494.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3406
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.guided.289"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            complement(<1..613)
                     /gene="tetR(A)"
                     /locus_tag="KLK57_004672"
     CDS             complement(<1..613)
                     /gene="tetR(A)"
                     /locus_tag="KLK57_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(A)"
                     /protein_id="HBE0894807.1"
                     /translation="MTKLQPNTVIRAALDLLNEVGVDGLTTRKLAERLGVQQPALYWH
                     FRNKRALLDALAEAMLAENHTHSVPRADDDWRSFLIGNARSFRQALLAYRDGARIHAG
                     TRPGAPQMETADAQLRFLCEAGFSAGDAVNALMTISYFTVGAVLEEQAGDSDAGERGG
                     TVEQAPLSPLLRAAIDAFDEAGPDAAFEQGLAVIVDGLAKRRLV"
     gene            719..1918
                     /gene="tet(A)"
                     /locus_tag="KLK57_004673"
     CDS             719..1918
                     /gene="tet(A)"
                     /locus_tag="KLK57_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(A)"
                     /protein_id="HBE0894808.1"
                     /translation="MKPNRPLIVILSTVALDAVGIGLIMPVLPGLLRDLVHSNDVTAH
                     YGILLALYALMQFACAPVLGALSDRFGRRPVLLVSLAGAAVDYAIMATAPFLWVLYIG
                     RIVAGITGATGAVAGAYIADITDGDERARHFGFMSACFGFGMVAGPVLGGLMGGFSPH
                     APFFAAAALNGLNFLTGCFLLPESHKGERRPLRREALNPLASFRWARGMTVVAALMAV
                     FFIMQLVGQVPAALWVIFGEDRFHWDATTIGISLAAFGILHSLAQAMITGPVAARLGE
                     RRALMLGMIADGTGYILLAFATRGWMAFPIMVLLASGGIGMPALQAMLSRQVDEERQG
                     QLQGSLAALTSLTSIVGPLLFTAIYAASITTWNGWAWIAGAALYLLCLPALRRGLWSG
                     AGQRADR"
     gene            complement(1950..2834)
                     /locus_tag="KLK57_004674"
     CDS             complement(1950..2834)
                     /locus_tag="KLK57_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HBE0894809.1"
                     /translation="MSLRTPDLLFTAIAPAIWGSTYIVTTQYLPNFSPMTVAMLRALP
                     AGLLLVMIVRQIPTGIWWMRIFILGALNISLFWSLLFISVYRLPGGVAATVGAVQPLM
                     VVFISAALLGSPIRLMAVLGAICGTAGVALLVLTPNAALDPVGVAAGLAGAVSMAFGT
                     VLTRKWQPPVPLLTFTAWQLAAGGLLLVPVALVFDPPIPMPTGTNVLGLAWLGLIGAG
                     LTYFLWFRGISRLEPTVVSLLGFLSPGTAVLLGWLFLDQTLSALQIIGVLLVIGSIWL
                     GQRSNRTPRARIACRKSP"
     gene            complement(2972..3379)
                     /locus_tag="KLK57_004675"
     CDS             complement(2972..3379)
                     /locus_tag="KLK57_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HBE0894810.1"
                     /translation="MRGRPVLFPNFSLGEEYEHAPPATNRQISPYLPSGRFRTGLPVE
                     GLAIERGDLFYACPRASVFYGTALDADLRTRGVSTLVMAGISTTGVVLSSVAWASDAD
                     YDVRLVQDCCYDPDRDAHEALLRSGFGGRVQVV"
BASE COUNT          653 a         1073 c         1038 g          642 t
ORIGIN      
        1 caacgagcct ccttttcgcc aatccatcga caatcaccgc gagtccctgc tcgaacgctg
       61 cgtccggacc ggcttcgtcg aaggcgtcta tcgcggcccg caacagcggc gagagcggag
      121 cctgttcaac ggtgccgccg cgctcgccgg catcgctgtc gccggcctgc tcctcaagca
      181 cggccccaac agtgaagtag ctgattgtca tcagcgcatt gacggcgtcc ccggccgaaa
      241 aacccgcctc gcagaggaag cgaagctgcg cgtcggccgt ttccatctgc ggtgcgcccg
      301 gtcgcgtgcc ggcatggatg cgcgcgccat cgcggtaggc gagcagcgcc tgcctgaagc
      361 tgcgggcatt cccgatcaga aatgagcgcc agtcgtcgtc ggctctcggc accgaatgcg
      421 tatgattctc cgccagcatg gcttcggcca gtgcgtcgag cagcgcccgc ttgttcctga
      481 agtgccagta aagcgccggc tgctgaaccc ccaaccgttc cgccagtttg cgtgtcgtca
      541 gaccgtctac gccgacctcg ttcaacaggt ccagggcggc acggatcact gtattcggct
      601 gcaactttgt catgcttgac actttatcac tgataaacat aatatgtcca ccaacttatc
      661 agtgataaag aatccgcgcg ttcaatcgga ccagcggagg ctggtccgga ggccagacgt
      721 gaaacccaac agacccctga tcgtaattct gagcactgtc gcgctcgacg ctgtcggcat
      781 cggcctgatt atgccggtgc tgccgggcct cctgcgcgat ctggttcact cgaacgacgt
      841 caccgcccac tatggcattc tgctggcgct gtatgcgttg atgcaatttg cctgcgcacc
      901 tgtgctgggc gcgctgtcgg atcgtttcgg gcggcggccg gtcttgctcg tctcgctggc
      961 cggcgctgct gtcgactacg ccatcatggc gacggcgcct ttcctttggg ttctctatat
     1021 cgggcggatc gtggccggca tcaccggggc gactggggcg gtagccggcg cttatattgc
     1081 cgatatcact gatggcgatg agcgcgcgcg gcacttcggc ttcatgagcg cctgtttcgg
     1141 gttcgggatg gtcgcgggac ctgtgctcgg tgggctgatg ggcggtttct ccccccacgc
     1201 tccgttcttc gccgcggcag ccttgaacgg cctcaatttc ctgacgggct gtttcctttt
     1261 gccggagtcg cacaaaggcg aacgccggcc gttacgccgg gaggctctca acccgctcgc
     1321 ttcgttccgg tgggcccggg gcatgaccgt cgtcgccgcc ctgatggcgg tcttcttcat
     1381 catgcaactt gtcggacagg tgccggccgc gctttgggtc attttcggcg aggatcgctt
     1441 tcactgggac gcgaccacga tcggcatttc gcttgccgca tttggcattc tgcattcact
     1501 cgcccaggca atgatcaccg gccctgtagc cgcccggctc ggcgaaaggc gggcactcat
     1561 gctcggaatg attgccgacg gcacaggcta catcctgctt gccttcgcga cacggggatg
     1621 gatggcgttc ccgatcatgg tcctgcttgc ttcgggtggc atcggaatgc cggcgctgca
     1681 agcaatgttg tccaggcagg tggatgagga acgtcagggg cagctgcaag gctcactggc
     1741 ggcgctcacc agcctgacct cgatcgtcgg acccctcctc ttcacggcga tctatgcggc
     1801 ttctataaca acgtggaacg ggtgggcatg gattgcaggc gctgccctct acttgctctg
     1861 cctgccggcg ctgcgtcgcg ggctttggag cggcgcaggg caacgagccg atcgctgatc
     1921 gtggaaacga taggcctatg ccatgcgggt caaggcgact tccggcaagc tatacgcgcc
     1981 ctaggagtgc ggttggaacg ttggcccagc cagatactcc cgatcacgag caggacgccg
     2041 atgatttgaa gcgcactcag cgtctgatcc aagaacaacc atcctagcaa cacggcggtc
     2101 cccgggctga gaaagcccag taaggaaaca actgtaggtt cgagtcgcga gatcccccgg
     2161 aaccaaagga agtaggttaa acccgctccg atcaggccga gccacgccag gccgagaaca
     2221 ttggttcctg taggcatcgg gattggcgga tcaaacacta aagctactgg aacgagcaga
     2281 agtcctccgg ccgccagttg ccaggcggta aaggtgagca gaggcacggg aggttgccac
     2341 ttgcgggtca gcacggttcc gaacgccatg gaaaccgccc ccgccaggcc cgctgcgacg
     2401 ccgacaggat ctagcgctgc gtttggtgtc aacaccaaca gcgccacgcc cgcagttccg
     2461 caaatagccc ccaggaccgc catcaatcgt atcgggctac ctagcagagc ggcagagatg
     2521 aacacgacca tcagcggctg cacagcgcct accgtcgccg cgaccccgcc cggcaggcgg
     2581 tagaccgaaa taaacaacaa gctccagaat agcgaaatat taagtgcgcc gaggatgaag
     2641 atgcgcatcc accagattcc cgttggaatc tgtcggacga tcatcacgag caataaaccc
     2701 gccggcaacg cccgcagcat cgcgaccgtc atcggtgaga agttcggcag gtattgggtg
     2761 gtgacaatgt aggtgctgcc ccaaatggca ggtgctatcg ctgtgaacaa taaatcgggc
     2821 gtgcgtaatg acatgcgatt acaagacctc cgctacggcg atgggttttt gagagttgca
     2881 ggcgccgcga tgaccgcacc ctgacttgga ctggccctgc ctcgcaccaa tccacctgcg
     2941 gctatggccg cgatcatgcg aggcccgaaa attacacgac ctgtacacgt ccgccgaacc
     3001 cggaacgcaa cagagcttcg tgggcatccc gatccgggtc gtagcagcag tcctggacca
     3061 aacgcacgtc gtagtccgca tcactagccc aggcgacgct tgaaagaaca acgccggtgg
     3121 tgcttatccc ggccatgaca agcgtgctta cgccgcgcgt ccgaaggtcg gcgtcgagcg
     3181 ccgtgccata aaagacgctg gctcgcggac atgcatagaa aaggtcgccc cgttcgatcg
     3241 caagcccttc gacgggcaga ccggtgcgaa atcgtccgct cgggagatac ggtgagattt
     3301 ggcgattcgt cgccggcggc gcatgttcat actcttcgcc gagcgaaaag ttggggaaca
     3361 gcacgggccg gccgcgcaag gcgtgctggc agccgtgcag accctg
//
LOCUS       DAEEKQ010000495         2329 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.guided.337, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000495 DAEEKQ010000000
VERSION     DAEEKQ010000495.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2329
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.guided.337"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..699
                     /gene="erm(B)"
                     /locus_tag="KLK57_004676"
     CDS             <1..699
                     /gene="erm(B)"
                     /locus_tag="KLK57_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HBE0894811.1"
                     /translation="SEKVLNQIIKQLNLKETDTVYEIGTGKGHLTTKLAKISKQVTSI
                     ELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIPYHLSTQIIKKVVF
                     ESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAECFHPKPRVNSVLI
                     KLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHAKVNNLSTVTYEQV
                     LSIFNSYLLFNGRK"
     gene            644..814
                     /locus_tag="KLK57_004677"
     CDS             644..814
                     /locus_tag="KLK57_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HBE0894812.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDKLLGIL
                     LTASKELKRSLD"
     gene            1329..1778
                     /locus_tag="KLK57_004678"
     CDS             1329..1778
                     /locus_tag="KLK57_004678"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894813.1"
                     /translation="MTTILSDYNKSILIELLNTNRQIIVVHGDDIDDYYTDSCYDIIF
                     IDYYDDDDFTYNGQEIWRGDSIYLVKSYQDEIQNRIEKGQELIFVNKENDISFSESIL
                     TYYHLFDYAGKTFEIDGQIIDEQTKVFAAPERRCACRTCWKVPARKT"
     gene            1739..2080
                     /locus_tag="KLK57_004679"
     CDS             1739..2080
                     /locus_tag="KLK57_004679"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HBE0894814.1"
                     /translation="MSHVLEGASQEDLNLYRAEVERDQAYGNWRDFFLNKKGSVTSVS
                     GDANLDQIADVSYLLDTFFAKVTRTALQNAASIAGLMITTEAMVAEAPKKDEPAMPPG
                     GGMGGMGGMDF"
BASE COUNT          739 a          495 c          486 g          609 t
ORIGIN      
        1 agtgaaaaag tactcaacca aataataaaa caattgaatt taaaagaaac cgataccgtt
       61 tacgaaattg gaacaggtaa agggcattta acgacgaaac tggctaaaat aagtaaacag
      121 gtaacgtcta ttgaattaga cagtcatcta ttcaacttat cgtcagaaaa attaaaactg
      181 aatactcgtg tcactttaat tcaccaagat attctacagt ttcaattccc taacaaacag
      241 aggtataaaa ttgttgggaa tattccttac catttaagca cacaaattat taaaaaagtg
      301 gtttttgaaa gccatgcgtc tgacatctat ctgattgttg aagaaggatt ctacaagcgt
      361 accttggata ttcaccgaac actagggttg ctcttgcaca ctcaagtctc gattcagcaa
      421 ttgcttaagc tgccagcgga atgctttcat cctaaaccaa gagtaaacag tgtcttaata
      481 aaacttaccc gccataccac agatgttcca gataaatatt ggaagctata tacgtacttt
      541 gtttcaaaat gggtcaatcg agaatatcgt caactgttta ctaaaaatca gtttcatcaa
      601 gcaatgaaac acgccaaagt aaacaattta agtaccgtta cttatgagca agtattgtct
      661 atttttaata gttatctatt atttaacggg aggaaataat tctatgagtc gcttttgtaa
      721 atttggaaag ttacacgtta ctaaagggaa tgtagataaa ttattaggta tactactgac
      781 agcttccaag gagctaaaga ggtccctaga ctagcaagaa gtacacaaga agccttaaag
      841 attatagaaa agcttttcca tgaaatgctt aacattattt aatgttaaca tgtggctttg
      901 tggtaactac cacgagtggt aacttgtaac atgaaaaaag ttacaagtta caagtggttt
      961 atttacgaac ataaaggcta ggaaagcact tgataagtat atcaagtgct ttttttccgt
     1021 tcgattttac tatccaagat accgcttgac gtacctaata ttttagtgtg ctaagggctt
     1081 tgctttttct ggttatagtt cgttcaaaaa agaccgcaag ggtaaatgca cgatcataga
     1141 tcgcccttgc agctttttaa tctctccact tttttataac caagcaaacc accaagcaca
     1201 ctgaaaaaat aattaggaac taaagcgaag tgcgtatctt ggataaatct tcactcgatt
     1261 ttaaaagatt aagcacccag ccaaagcgcc taaaaaaata atttttttaa agaaaggaag
     1321 taatttaaat gactacaatt ttaagcgact acaataaatc tatcctaatt gaattactaa
     1381 acaccaatcg ccaaattatc gtcgttcatg gcgacgatat tgatgattat tacacagatt
     1441 cttgctatga cattatcttt attgattact atgatgatga tgatttcact tataacggac
     1501 aggaaatatg gcgtggcgat agcatttatc ttgtaaaaag ttatcaagat gaaattcaaa
     1561 accgtataga aaaaggacaa gagctgatat ttgttaataa agaaaatgat atatcatttt
     1621 cagaatctat tttaacttat taccatctat ttgattatgc tggtaaaacg tttgaaatag
     1681 acggtcaaat cattgatgaa caaacaaaag tttttgccgc acccgagcgc cgctgcgcat
     1741 gtcgcacgtg ctggaaggtg ccagccagga agacctgaac ctttaccgcg cggaagtgga
     1801 gcgcgaccag gcctatggca actggcgcga tttcttcctg aacaagaagg gcagcgtcac
     1861 atccgtgagc ggtgatgcca acctcgacca gatcgcggac gtgtcgtacc tgctggatac
     1921 cttctttgcc aaggtcaccc gcaccgcgtt gcagaacgcc gcctccatcg cgggcctgat
     1981 gatcaccacc gaagccatgg tggccgaggc cccgaagaag gacgagccgg cgatgccgcc
     2041 gggcggcggc atgggtggca tgggcggcat ggatttctga tgcggttggc ccggtcgtca
     2101 gggaaccgga ccgcgccagc gcggtccgat cccggcaacg acccgacatc aaggccccaa
     2161 ggacggggcc ggagcccggc agcgatgccg ggctttttgt tgtgcccgcg ccgcggcaat
     2221 gtctgacgcg aagatcagaa cgcaccgata cgaacgtgcg aacacaggtg caaccctgag
     2281 cagccgtccc cgcaccggag cgctgcgtgc cgcgcctcgc cacatcccg
//
LOCUS       DAEEKQ010000496         2280 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.guided.339, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000496 DAEEKQ010000000
VERSION     DAEEKQ010000496.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2280
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.guided.339"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            <1..371
                     /locus_tag="KLK57_004680"
     CDS             <1..371
                     /locus_tag="KLK57_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HBE0894815.1"
                     /translation="ISLSPPTICNSALSEKVVIYYEKRGNAENYIKEAKYDMAVGHLL
                     LKSFWANEAVFQMMMLSYNLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTARY
                     VIMKLSENYPYKGVYEKCLV"
     gene            621..1496
                     /locus_tag="KLK57_004681"
     CDS             621..1496
                     /locus_tag="KLK57_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A extended-spectrum beta-lactamase
                     CTX-M-27"
                     /protein_id="HBE0894816.1"
                     /translation="MVTKRVQRMMFAAAACIPLLLGSAPLYAQTSAVQQKLAALEKSS
                     GGRLGVALIDTADNTQVLYRGDERFPMCSTSKVMAAAAVLKQSETQKQLLNQPVEIKP
                     ADLVNYNPIAEKHVNGTMTLAELSAAALQYSDNTAMNKLIAQLGGPGGVTAFARAIGD
                     ETFRLDRTEPTLNTAIPGDPRDTTTPRAMAQTLRQLTLGHALGETQRAQLVTWLKGNT
                     TGAASIRAGLPTSWTVGDKTGSGGYGTTNDIAVIWPQGRAPLVLVTYFTQPQQNAESR
                     RDVLASAARIIAEGL"
     gene            1531..>2280
                     /locus_tag="KLK57_004682"
     CDS             1531..>2280
                     /locus_tag="KLK57_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HBE0894817.1"
                     /translation="MKDQITYLPDNADRSVAKQKFKITNWPTYNKALINRGSITFWLD
                     DEAIQAWYESATPSSRGRPQRYSDLAITTVLVIKRVFRLTLRAAQGFIDSIFSLMNVP
                     LRCPDYSCVSRRAKSVNISFKTPTRGEIAHLVIDSTGLKVFGEGEWKVKKHGQERRRI
                     WRKLHLAVDSKTHEIICADLSLNNVTDSEAFPGLIRQTHRKIRSAAADGAYDTRLCHD
                     ELRRKKISALIPPRKGAGYGTVAKLAMRQPFV"
BASE COUNT          602 a          541 c          612 g          525 t
ORIGIN      
        1 tgataagttt atcaccaccg actatttgca acagtgccct ttctgaaaaa gtagttatat
       61 actatgaaaa gcgtggtaat gctgaaaact atatcaaaga agccaaatac gacatggcgg
      121 tgggtcatct cttgctaaag tcattttggg cgaatgaagc cgtgtttcaa atgatgatgc
      181 tttcatataa cctatttttg ttgttcaagt ttgattcctt ggactcttca gaatacagac
      241 agcaaataaa gacctttcgt ttgaagtatg tatttcttgc agcaaaaata atcaaaaccg
      301 caagatatgt aatcatgaag ttgtcggaaa actatccgta caagggagtg tatgaaaaat
      361 gtctggtata ataagaatat catcaataaa attgagtgtt gctctgtgga taacttgcag
      421 agtttattaa gtatcattgc agcaaagatg aaatcaatga tttatcaaaa atgattgaaa
      481 ggtggttgta aataatgtta caatgtgtga gaagcagtct aaattcttcg tgaaatagtg
      541 atttttgaag ctaataaaaa acacacgtgg aatttaggga atactgatgt aacacggatt
      601 gaccgtattg ggagtttgag atggtgacaa agagagtgca acggatgatg ttcgcggcgg
      661 cggcgtgcat tccgctgctg ctgggcagcg cgccgcttta tgcgcagacg agtgcggtgc
      721 agcaaaagct ggcggcgctg gagaaaagca gcggagggcg gctgggcgtc gcgctcatcg
      781 ataccgcaga taatacgcag gtgctttatc gcggtgatga acgctttcca atgtgcagta
      841 ccagtaaagt tatggcggcc gcggcggtgc ttaagcagag tgaaacgcaa aagcagctgc
      901 ttaatcagcc tgtcgagatc aagcctgccg atctggttaa ctacaatccg attgccgaaa
      961 aacacgtcaa cggcacaatg acgctggcag aactgagcgc ggccgcgttg cagtacagcg
     1021 acaataccgc catgaacaaa ttgattgccc agctcggtgg cccgggaggc gtgacggctt
     1081 ttgcccgcgc gatcggcgat gagacgtttc gtctggatcg cactgaacct acgctgaata
     1141 ccgccattcc cggcgacccg agagacacca ccacgccgcg ggcgatggcg cagacgttgc
     1201 gtcagcttac gctgggtcat gcgctgggcg aaacccagcg ggcgcagttg gtgacgtggc
     1261 tcaaaggcaa tacgaccggc gcagccagca ttcgggccgg cttaccgacg tcgtggactg
     1321 tgggtgataa gaccggcagc ggcggctacg gcaccaccaa tgatattgcg gtgatctggc
     1381 cgcagggtcg tgcgccgctg gttctggtga cctattttac ccagccgcaa cagaacgcag
     1441 agagccgccg cgatgtgctg gcttcagcgg cgagaatcat cgccgaaggg ctgtaactgg
     1501 ttttgttgaa taaatcgaac ttttgctgag ttgaaggatc agatcacgta tcttcccgac
     1561 aacgcagacc gttccgtggc aaagcaaaag ttcaaaatca ccaactggcc cacctacaat
     1621 aaagccctca tcaaccgtgg ctccataact ttctggctgg atgatgaagc tattcaggcc
     1681 tggtatgagt cagcaacacc ttcttcacga ggcagacctc agcgctattc tgaccttgcc
     1741 atcacgactg tgctggtcat taaacgcgta ttcaggctga ccctgcgcgc tgcgcagggc
     1801 tttattgatt ccattttttc tctgatgaac gttccgctac gctgcccgga ttacagctgt
     1861 gtcagcaggc gggcaaagtc ggttaatatc agtttcaaaa cgcccacccg gggtgaaatc
     1921 gcacacctgg taattgattc caccgggctg aaggtcttcg gtgaaggcga gtggaaagtc
     1981 aaaaagcatg gccaggaacg ccgtcgtata tggcgtaagc tgcatctggc agttgacagt
     2041 aaaacacatg aaatcatctg cgctgacctg tcgctgaaca acgttacgga ctcagaggcc
     2101 ttccccgggt taatccggca aacccaccgg aaaatcaggt cagccgccgc cgatggagct
     2161 tacgataccc ggctctgtca cgatgaactg cggcgtaaga aaatcagcgc gcttatcccg
     2221 ccccgaaaag gtgcgggtta cggcactgtt gcaaagttag cgatgaggca gccttttgtc
//
LOCUS       DAEEKQ010000497         1680 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.guided.367, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000497 DAEEKQ010000000
VERSION     DAEEKQ010000497.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.guided.367"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            109..1023
                     /locus_tag="KLK57_004683"
     CDS             109..1023
                     /locus_tag="KLK57_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mph(A) family macrolide 2'-phosphotransferase"
                     /protein_id="HBE0894818.1"
                     /translation="MTVVTTADTSQLYALAARHGLKLHGPLTVNELGLDYRIVIATVD
                     DGRRWVLRIPRRAEVSAKVEPEARVLAMLKNRLPFAVPDWRVANAELVAYPMLEDSTA
                     MVIQPGSSTPDWVVPQDSEVFAESFATALAALHAVPISAAVDAGMLIRTPTQARQKVA
                     DDVDRVRREFVVNDKRLHRWQRWLDDDSSWPDFSVVVHGDLYVGHVLIDNTERVSGMI
                     DWSEARVDDPAIDMAAHLMVFGEEGLAKLLLTYEAAGGRVWPRLAHHIAERLAFGAVT
                     YALFALDSGNEEYLAAAKAQLAAAEAAE"
     gene            1020..>1680
                     /locus_tag="KLK57_004684"
     CDS             1020..>1680
                     /locus_tag="KLK57_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HBE0894819.1"
                     /translation="MSERRYSPLATLFAATFLFRIGNAVAALALPWFVLSHTKSAAWA
                     GATAASSVIATIIGAWVGGGLVDRFGRAPVALISGVVGGVAMASIPLLDAVGALSNTG
                     LIACVVLGAAFDAPGMAAQDSELPKLGHVAGLSVERVSSLKAVIGNVAILGGPALGGA
                     AIGLLGAAPTLGLTAFCSVLAGLLGAWVLPARAARTMTTTATLSMRAGVAFLWSEPLL
                     RP"
BASE COUNT          257 a          551 c          553 g          319 t
ORIGIN      
        1 gacaaaaggc tgcctcatcg ctaactttgc aacagtgccg gattgaatat aaccgacgtg
       61 actgttacat ttaggtggct aaacccgtca agccctcagg agtgaatcat gaccgtagtc
      121 acgaccgccg atacctccca actgtacgca cttgcagccc gacatgggct caagctccat
      181 ggcccgctga ctgtcaatga gcttgggctc gactatagga tcgtgatcgc caccgtcgac
      241 gatggacgtc ggtgggtgct gcgcatcccg cgccgagccg aggtaagcgc gaaggtcgaa
      301 ccagaggcgc gggtgctggc aatgctcaag aatcgcctgc cgttcgcggt gccggactgg
      361 cgcgtggcca acgccgagct cgttgcctat cccatgctcg aagactcgac tgcgatggtc
      421 atccagcctg gttcgtccac gcccgactgg gtcgtgccgc aggactcgga ggtcttcgcg
      481 gagagcttcg cgaccgcgct cgccgccctg catgccgtcc ccatttccgc cgccgtggat
      541 gcggggatgc tcatccgtac accgacgcag gcccgtcaga aggtggccga cgacgttgac
      601 cgcgtccgac gcgagttcgt ggtgaacgac aagcgcctcc accggtggca gcgctggctc
      661 gacgacgatt cgtcgtggcc agatttctcc gtggtggtgc atggcgatct ctacgtgggc
      721 catgtgctca tcgacaacac ggagcgcgtc agcgggatga tcgactggag cgaggcccgc
      781 gttgatgacc ctgccatcga catggccgcg caccttatgg tctttggtga agaggggctc
      841 gcgaagctcc tcctcacgta tgaagcggcc ggtggccggg tgtggccgcg gctcgcccac
      901 cacatcgcgg agcgccttgc gttcggggcg gtcacctacg cactcttcgc cctcgactcg
      961 ggtaacgaag agtacctcgc tgcggcgaag gcgcagctcg ccgcagcgga agcagcggaa
     1021 tgagcgaacg tcgatatagc ccgctcgcga cgctgttcgc ggcgaccttt ctcttccgga
     1081 tcggcaacgc ggtggcggcc ctcgcgcttc catggttcgt cctgtctcat acaaagagcg
     1141 cggcctgggc gggcgccacg gccgctagca gcgtcatcgc gaccatcatc ggcgcgtggg
     1201 ttggtggtgg cctcgtcgat cggttcgggc gcgcgcccgt cgcattgatc tcgggtgtgg
     1261 tgggcggcgt ggccatggcg agcatcccac tgctcgatgc cgttggcgcc ctctcgaaca
     1321 ctgggctgat cgcttgcgtg gtgctcggtg ccgcgttcga cgcacccggt atggccgcgc
     1381 aggacagtga gctgcccaaa ctcggccacg tcgccgggct ctccgttgag cgcgtctcgt
     1441 cactgaaagc ggtgatcggg aacgtcgcga ttctaggtgg cccggccctt gggggggccg
     1501 caatcggcct gcttggcgct gcgccaacgc tcgggctgac ggcgttctgc tccgtccttg
     1561 caggtctgct cggcgcgtgg gtgcttcccg cgcgtgccgc tcggacgatg accacgacgg
     1621 cgactctctc catgcgcgcc ggcgtcgctt ttctctggag cgaacccctg ctgcgccctc
//
LOCUS       DAEEKQ010000498          786 bp    DNA     linear   BCT 26-MAY-2021
DEFINITION  TPA_asm: Shigella sonnei strain SS047291
            SAMN15300358-rid12250483.guided.450, whole genome shotgun sequence.
ACCESSION   DAEEKQ010000498 DAEEKQ010000000
VERSION     DAEEKQ010000498.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN15300358
            Sequence Read Archive: SRR12072370
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella sonnei
  ORGANISM  Shigella sonnei
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 786)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (19-MAY-2021) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 19-MAY-2021
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001042324.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001042324.1
            Genome Coverage       :: 75x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/19/2021 21:48:09
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,686
            CDSs (total)                      :: 4,593
            Genes (coding)                    :: 4,325
            CDSs (with protein)               :: 4,325
            Genes (RNA)                       :: 93
            rRNAs                             :: 2, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 78
            ncRNAs                            :: 9
            Pseudo Genes (total)              :: 268
            CDSs (without protein)            :: 268
            Pseudo Genes (ambiguous residues) :: 0 of 268
            Pseudo Genes (frameshifted)       :: 123 of 268
            Pseudo Genes (incomplete)         :: 83 of 268
            Pseudo Genes (internal stop)      :: 96 of 268
            Pseudo Genes (multiple problems)  :: 33 of 268
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..786
                     /organism="Shigella sonnei"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN15300358-rid12250483.guided.450"
                     /strain="SS047291"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:624"
                     /country="Canada: Montreal"
                     /lat_lon="45.50 N 73.57 W"
                     /collection_date="2018-01"
                     /collected_by="LSPQ"
     gene            134..607
                     /locus_tag="KLK57_004685"
     CDS             134..607
                     /locus_tag="KLK57_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HBE0894820.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            702..>786
                     /locus_tag="KLK57_004686"
     CDS             702..>786
                     /locus_tag="KLK57_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat1"
                     /protein_id="HBE0894821.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDV"
BASE COUNT          246 a          150 c          173 g          217 t
ORIGIN      
        1 gtaataacag agtgtcttgt atttttaaag aaagtctatt taatacaagt gattatatta
       61 attaacggta agcatcagcg ggtgacaaaa cgagcatgct tactaataaa atgttaacct
      121 ctgaggaaga attgtgaaac tatcactaat ggtagctata tcgaagaatg gagttatcgg
      181 gaatggccct gatattccat ggagtgccaa aggtgaacag ctcctgttta aagctattac
      241 ctataaccaa tggctgttgg ttggacgcaa gacttttgaa tcaatgggag cattacccaa
      301 ccgaaagtat gcggtcgtaa cacgttcaag ttttacatct gacaatgaga acgtattgat
      361 ctttccatca attaaagatg ctttaaccaa cctaaagaaa ataacggatc atgtcattgt
      421 ttcaggtggt ggggagatat acaaaagcct gatcgatcaa gtagatacac tacatatatc
      481 tacaatagac atcgagccgg aaggtgatgt ttactttcct gaaatcccca gcaattttag
      541 gccagttttt acccaagact tcgcctctaa cataaattat agttaccaaa tctggcaaaa
      601 gggttaacaa gtggcagcaa cggattcgca aacctgtcac gccttttgta ccaaaagccg
      661 cgccaggttt gcgatccgct gtgccaggcg ttaggcgtca tatgaagatt tcggtgatcc
      721 ctgagcaggt ggcggaaaca ttggatgctg agaaccattt cattgttcgt gaagtgttcg
      781 atgtgc
//
